LOCUS       DATMRM010000001        17382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4339065, whole genome shotgun sequence.
ACCESSION   DATMRM010000001 DATMRM010000000
VERSION     DATMRM010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17382
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4339065"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VNZ06_00005"
     CDS             <1..369
                     /locus_tag="VNZ06_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017217706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWW19159.1"
                     /translation="DYDEALRFYVGTLGFSLIEDTYVSAQDKRWVVVAPSRTSGARVL
                     LARASNPTQLSTVGNQSGGRVFLFLHTDDLLRDYDRYRARGVEFVRPPKDESYGKVAV
                     FKDLYGNLWDLIQPSAQHAG"
     gene            400..834
                     /locus_tag="VNZ06_00010"
     CDS             400..834
                     /locus_tag="VNZ06_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3995 domain-containing protein"
                     /protein_id="HWW19160.1"
                     /translation="MNPLLPLSVSLIFVVLALWHFYMASRPSQTVSGAVPSAGGKPLF
                     VPGRVATVAVGVALLVCAALVAATGGVIPRGLPVRMLSLLSYALAAGLLLRAVGEFKY
                     LGFFKRVRGTRFARLDTLLYSPLCLALAVGVTWVAYAGGAMS"
     gene            974..1291
                     /locus_tag="VNZ06_00015"
     CDS             974..1291
                     /locus_tag="VNZ06_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19161.1"
                     /translation="MRQAPRTLTRCNCSICRRYGAIWAYYKPTSVRIEAPAHGLMRYS
                     WNRKVRDYHRCRHCGCVMHYTYRKKNAWNTVGVNASNFDPTIVASARIRNLDGAKSWR
                     FLE"
     gene            complement(1483..2949)
                     /gene="zwf"
                     /locus_tag="VNZ06_00020"
     CDS             complement(1483..2949)
                     /gene="zwf"
                     /locus_tag="VNZ06_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWW19162.1"
                     /translation="MAAKNNAGVGPQYPQVLVLFGATGDLSQRKLLPGLFHLSDAGFI
                     PGCRIIGVSLDDIDTEGFRKVARKALDDFSTRKFAEEAWSAFAQSLDYVPLSAGASTL
                     KAAVERAEKTFSGESRRLHYLSVPPNAALSAVRMLGEAGLVERSRIIMEKPFGTDLAS
                     AVALNANLHAVFDEEQIFRIDHFLGKEPAQNILAFRFANGLFEPIWNRNFINHVQIDV
                     PETLSLGKRVGFYEQTGAYRDMVVTHLFQILAFMAMEPPTALDPKPISEEKNKVFRSM
                     EPIEPHQVVRGQYSGYRSEQGVDPRSDTETFIALRCSIDNWRWAGVPFYLRTGKRLAE
                     GQRIISIAFREPPKSMFPPGSGIGEQGPDHLTFDLADASKVSLSFYGKRPGPGMRLDK
                     LSLQFAMHETGLIGDVLEAYERLILDAMRGDHTLFTTAEGIERLWEVSAPLLNAPPPV
                     RSYAPGSWGPNAIHQLIAPHAWRLPFERVWRAPNPAGA"
     gene            complement(3112..5292)
                     /locus_tag="VNZ06_00025"
     CDS             complement(3112..5292)
                     /locus_tag="VNZ06_00025"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HWW19163.1"
                     /translation="MSDRVTQGELQVARVLYDFVNQEALPGTGINPESFWKGFDQLMR
                     DLAPRNAQLLQRRDQLQAQIDQWHQAHPGPKFDHQAYRGFLEQIGYLEPAGESFTIET
                     QLVDDEIATIAGPQLVVPVDNARYALNAANARWGSLYDALYGTDVIPEDQGATRAGAY
                     NPKRGAKVIAYVRAFLDEHFPLNAGSHAQAIAYAVSAGGLSVKFTDGKTATLRNGGAL
                     VGFQAEPGAPSAVLLKHHGLHVEIQIDRSHAIGRTDAAGVADVVLEAAITTIEDCEDS
                     VAAVDADDKVRVYRNWLGLMKGTLSAEFDKGGKSVRRSLNPDRSYRTPSGGALTLNGR
                     SLMLVRNVGHFMMSDAITMQGKEVPETLIDAVVTSLIAMHDLKSGSPLRNSRRGSVYI
                     VKPKMHGAPEVAFADELFARVETLLGLPRDTLKIGIMDEERRTSVNLQQCIRTARRRV
                     VFINTGFLDRTGDEIHTSMEAGAVMRKNDMKKAQWLDAYERQNVDIGLRCGLPGKAQI
                     GKGMWAMPDMMAAMLSAKIAHPRAGANTAWVPSPTAATLHAIHYHQADVFGIQAQLRS
                     RPQASLDEILQPPLADKPSWSAAEIEEELHNNTQSILGYVVRWIDSGVGCSKVPDYYD
                     TPLMEDRATLRISSQHIANWLHHRICTRTQVEQALRRMAIVVDKQNAAIPGYRAMSAD
                     YDHSIAFAAARDLIFKGREQANGYTEAILHARRRERKAAAKAAR"
     gene            5525..6589
                     /gene="hppD"
                     /locus_tag="VNZ06_00030"
     CDS             5525..6589
                     /gene="hppD"
                     /locus_tag="VNZ06_00030"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HWW19164.1"
                     /translation="MSPDIHDNPLGTDGFEFVEFTSPDPIALGTIFEAMGFTAVARHR
                     SKNVLRYKQGDIDFILNMEPSGQAAAFRGAHGPSINAMAFRVRDAAKALQLALERGAT
                     EVKGAVGPMELNIPAIQGIGGSYLYLVDRYGAHEIYDVDFVPIAGADAMEAVGSTGLR
                     YIDHLTHNVHRGNMKVWAEFYERVFNFREIRYFDIEGKVTGLISRAMTSPDGKIRIPL
                     NESQDDRSQIEEFLHVYRGEGIQHVALGSDDIFATVDRMRARGVQFQDTPESYYEAIE
                     TRVPGHGENLDRLRERSILIDGAPTRGQGLLLQIFTQNVVGPCFFEIIQRKGNEGFGE
                     GNFKALFESLELDQMRRGVL"
     gene            6586..7884
                     /gene="hmgA"
                     /locus_tag="VNZ06_00035"
     CDS             6586..7884
                     /gene="hmgA"
                     /locus_tag="VNZ06_00035"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HWW19165.1"
                     /translation="MSDTLAYLTGFASHLESESLPGALPAQNSPQRCPYGLYAEQISG
                     TAFTAPRAVNRRSWLYRIRPAVTHRPFQTLDGERMHNQFCDSPVSPNQLRWDPLPIPG
                     APTEFVDGIVTYAGTGEPGTHQGCGIHLYSANRSMTRRAFYDADGELLLLPQQGRVLL
                     YTEFGRLLVEPLEFAVIPRGVRFRVELLDGAARGYVCENFGAPLRLPELGPIGSNGLA
                     CERDFHVPTAWYEDSDGDYELLAKFDGKLWSVTLDHSPFDVVAWRGNHAPYKYDLRRF
                     NTIGSVSFDHPDPSIFTVLQSVSASAGIDDLAFVIFPPRWLAMEHTFRPPWFHRNVAS
                     EFMGLIRGAYDAKAQGFVPGGASLHNCMSGHGPDAQTFERASRADTSKPEHVMDTMAF
                     MFETRSVIRPTRAALESRLLQQNYYQCWQGLQKHFTPSQR"
     gene            7895..9223
                     /gene="fahA"
                     /locus_tag="VNZ06_00040"
     CDS             7895..9223
                     /gene="fahA"
                     /locus_tag="VNZ06_00040"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HWW19166.1"
                     /translation="MTLNETHDPQLRSWVASANLDDSDFPIQNLPFGVFRAQADIAPC
                     GGVAIGDQILSFGAAHRAGAFSPSDVGPLAARAAAAAAGSSLNEFMALGRESWSALRL
                     ALSRALREGATQRAMLQTCLVGQSRAQLLLPAQIGDYTDFYTSIYHATAVGKLMRPDN
                     PLFPNYRWIPIGYHGRGSSIGVSGQRFHRPCGQTLPRGAAAPVIGASQRLDYELELGV
                     FIGQGNESGTAIGIEDAEAHVFGLCLLNDWSARDLQGWEAQPLGPFLAKNFATTISPW
                     IVTLEALEPFRSAWQRPALDPQPLPYLEGAHLRERGAIDIELEVLLQSERMRERSQPP
                     TRVSGSNYRHAYWTLSQMVAHHSVNGCNLQPGDLLGTGTQSGPDPEQAGCLLELSQGG
                     KRAVRLPNGEERRFLEDGDEIVLRAWCERAGARRIGFGAAVGRVLPAHER"
     gene            9223..10020
                     /locus_tag="VNZ06_00045"
     CDS             9223..10020
                     /locus_tag="VNZ06_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014596135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HWW19167.1"
                     /translation="MYPQRIVCLTEEPTEVLYAIGEQDRIVGVSGFTVRPARVRREKP
                     RVSAFTSARIDRILELKPDLVVGFSDIQADIARDLIRHGVEVWISNHRSVQGILSYIR
                     RLGAMVGAAQKSEAYACSLEARLSQLAQAGAQLSQHPRVYFEEWDKPMISGIRWVSEL
                     VGIAGGVDVFPELAGCSLAKDRILCDSQEVVRRRPDIILGSWCGKRFRAEHVRARSGW
                     SEVPAVRDNELHEIKSPLILQPGPAALTDGVEAMHRIFARWAQQPRP"
     gene            10296..11279
                     /locus_tag="VNZ06_00050"
     CDS             10296..11279
                     /locus_tag="VNZ06_00050"
                     /inference="COORDINATES: protein motif:HMM:NF017051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19168.1"
                     /translation="MELCDFNVLHWRGARAPRAAVPQTRSGVDRFLIDTCQRRVAVVS
                     GREALDQARLEFPSDGAIELFAGADAYAFLLRFACGLESKLVAETEIFGQIKQAWREF
                     SGARSPLARQLSPWIQLLFQDAKAVRAQCLAKLGSASYGSQVRRLLAEDAKGGPTLLI
                     GAGQLAQSVAPWLTGSELWLWNRTSERAQELSRELAKRDPARPVRVIDSSADAELAAW
                     RAARNVILCVPADDPHDSARVQAWAQRSDRGGRVVHLGLGAQGATPWNDLPELVSLGA
                     LFEMLQAQTELRRRQIERARRACLEKALLRSLGANSSHPHSWEDLAAFHAI"
     gene            11283..12848
                     /gene="hemC"
                     /locus_tag="VNZ06_00055"
     CDS             11283..12848
                     /gene="hemC"
                     /locus_tag="VNZ06_00055"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWW19169.1"
                     /translation="MNSRVLKLGTRRSALARAQSAAVARLLQQHHPALAVELVGIETQ
                     GDRILDKPLSTVDGKEFFTAEIDAALLSGAVDLTVHSYKDLSLERSTRLLLAAVPKRE
                     PPGDIALFAPDVRQSLAAGEEIRVGSSSPRRASFVPDFLQRMLPADPMPARVRLVELR
                     GNVDSRLRRLHEPRGSPRHLDGIVLAFAGLARLWADSAGQQLLRELLTPLPRMLLPLS
                     ACPAAPAQGALAIECRSDDPDTARLLASIDDSATRRAVDAERALLAQRGGGCHQRFGA
                     TQIELSGLGALLYLRDADESGRPLPIPMVRWTPETPLPAPHGPLRAWDGSRADSAPAA
                     EPIPEAVARCAERLSSTRALFVAHRRALPEELTASVDPAAHVWVPGIETWQALAERGI
                     WVEGCGESLGFAALAPMLTQPSLQLPALDQWTVLTHAEAAGGWPATAVVATYRHASSS
                     PVGADPAGPAPDVTHVYWHSSAQFERWRASVAPGVHHACGSGRTSEHLRHAAVQNLRV
                     FPQVALWRQWLGL"
     gene            12833..13804
                     /locus_tag="VNZ06_00060"
     CDS             12833..13804
                     /locus_tag="VNZ06_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWW19170.1"
                     /translation="MVGIVTRSAGEGGLSVTPMRRTSWVAAAVAVWVTLQLVLVIWWA
                     ILIQRQAQRIASLETLSGAGHEQIAAQASRTHVMLVGESSTFLALLVVVTLLLAWLYW
                     REHQRARSMQAFFASVTHELRTPLTSIRLQAEAIAEGEHRAQLARRLLEDSSRLESQI
                     DKTLELARIEGGGPLSEQLIPLHTWIARSLEGIGAAFGDRLDLRAHVDGSVPAVFADA
                     GALQMILRNLVENSVRHSGATPVSVRLTAAQRGDYVVLEYQDNGNGLPGPSPRLGKLF
                     ARGVNSSGAGVGLYLVRRLMQRMGGRARFNTAPGEGYRCELSFRVGA"
     gene            13801..14514
                     /locus_tag="VNZ06_00065"
     CDS             13801..14514
                     /locus_tag="VNZ06_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWW19171.1"
                     /translation="MSGATRLLLVEDERNVAETLMERLRAEGFQVQRADSAASARRAI
                     AESEVEIALLDVGLPDGNGFELAKLLRERAPGTAIVFLTAHATPEDRVRGLELGADDY
                     IGKPFHFRELLLRINNTLKRAQELQRVPGDIRGQVRIGRAQVDFERFSANVGGESVAL
                     THKECAVLRLLAERVGKAVSRDEILDRAWSADEFPTSRTVDNFIVRLRKLIELNAADP
                     RVIRSIRGVGYLLTEINRE"
     gene            14518..15561
                     /locus_tag="VNZ06_00070"
     CDS             14518..15561
                     /locus_tag="VNZ06_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HWW19172.1"
                     /translation="MSETLLQQALARRNHHRPPVWFMRQAGRYHRHYQRLRARHEFID
                     LCKRPELAVEVTLGPMEEFGFDAAILFSDLLFPLEAMGMGLSYEPGPRLGWHLRSAAD
                     LTRLQGGADRAAHMSFQAEALSQLRARLDPYRALIGFVGGPFTLFAYAVAGSHEGFAR
                     DTLDDHDGTLYRGFIQKLQPLLAANMVLQVRGGADCIAIFDTAAGTLGAEGFARQVAP
                     VLRGLVSEFRAQCPQTPLIYYSRDTGPAHWDALRDIDFQCLGIDWRHDLVDVLNTYAD
                     RWALQGNIDPQWLLLSREQLEARLRNLFARVLLLPAAQRAAWVCGLGHGVLQHTPEDN
                     VRLALSIQREMFT"
     gene            15558..16919
                     /gene="hemN"
                     /locus_tag="VNZ06_00075"
     CDS             15558..16919
                     /gene="hemN"
                     /locus_tag="VNZ06_00075"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HWW19173.1"
                     /translation="MSVALGDPLIAKYDIASPRYTSYPTVPYWDSAPTADQWIEHIGR
                     ALQAAEASLGLYVHIPFCRTLCTFCGCNTRITRSHSIVGPYIQALLAELDLYRQRLGC
                     SELEFGELQLGGGTPTFLTVEELEALLSALYQRARPRRSAMIASVEVDPRVTDPTQLA
                     LLARYGFRSLSLGVQDFDPRVQDIVNRVQSEAQVQQLTEAARVLGFESVSYDLIYGLP
                     LQTRTSIDATMNALERLRPDRVALYAYAHVPWIKPGQRRFTDSDLPESEEKRELYELG
                     RERLTAQGYREIGLDHFALASDGLWQAQQAGRLHRNFMGYTSAYSRPIIGLGVSSIGD
                     AGDAFAQNEKDLQRYHERIANGELPLQRGHVLDTEDRILRRHILRLMTQRTTRWDQAA
                     EYTPFLATIGARLAEPAADELVLLEEKGCRITERGRPFLRNICMAFDARLARRMPEKL
                     PFR"
     gene            complement(16944..17357)
                     /locus_tag="VNZ06_00080"
     CDS             complement(16944..17357)
                     /locus_tag="VNZ06_00080"
                     /inference="COORDINATES: protein motif:HMM:NF015608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWW19174.1"
                     /translation="MLWVKSLHVIAVMAWLAGLFYLPRILVHYAEGQAAGEDVRRLAI
                     MARRLFGFMTMMGLLAILFGLWLWLGFHDTGRWLMVKLVFVLALLAYHLSCRALLMRI
                     TRGAPMPQPWVLRVLNESALLIVVPIILLAVMKPF"
BASE COUNT         2972 a         5436 c         5682 g         3292 t
ORIGIN      
        1 gactacgacg aggcgttgcg gttttacgtc ggaaccctgg ggttctcttt gatcgaggac
       61 acctacgtct cggcacagga caagcgctgg gtcgttgtgg ctccctcgcg aacgagcggc
      121 gcgcgcgtgc tgctggcacg ggcttcgaat ccgactcaac tgtccaccgt gggcaatcag
      181 agcggcggtc gcgtgtttct gttcctgcat accgacgatc tgcttcgcga ctacgaccgc
      241 taccgcgccc ggggggtcga gtttgttcgc cctcccaagg atgaatcgta cggaaaagtg
      301 gcggtgttca aggatctcta cggcaatctg tgggatctca tccagcccag tgcccagcat
      361 gctggctgag gcctccagcc gtgcgtcacc gcaagtgcca tgaaccctct gctgccgctg
      421 agtgtgtccc tgatcttcgt ggtgctcgca ctgtggcact tctatatggc gtcgcggccg
      481 agtcagacag tgagcggggc ggtcccgagt gctgggggca agccgttgtt cgtcccggga
      541 cgggtagcga ccgttgcggt cggcgtcgcg ttgctggtgt gtgcggcgct ggtggcggcg
      601 accgggggtg tgattccacg cgggctgccc gtacggatgt tgtcgctgtt gtcctatgca
      661 ctggcggccg gactgctgct gcgcgcagtc ggcgaattca aatatctggg tttcttcaag
      721 cgcgttcgcg gcacccgctt cgcacgtctg gacacgctgc tctattcgcc gctgtgcctg
      781 gcgcttgccg tcggcgtgac gtgggtagcg tatgcaggcg gagccatgag ctgagcgtca
      841 gcgcgcgcgg cgagggttgc ggccgccgat gcaagtaata agatgagggc ccgtgccaat
      901 accgcatacc caacgacgag ctacctctgc cagtgtcacc gccgtttgcc attgtggagc
      961 ggtgcgcgtt catgtgcgtc aggcgccgcg tacgctcacc cgatgcaatt gttcgatttg
     1021 tcgccgctat ggcgcgatct gggcctatta caagccaacg tccgtcagga tcgaggcgcc
     1081 ggctcacggc ctgatgcgct actcatggaa tcgcaaggtt cgtgactatc atcgctgtag
     1141 acactgcggc tgcgtcatgc actataccta tcgcaagaaa aatgcctgga ataccgtggg
     1201 agtcaatgcg tcgaacttcg atccgaccat cgttgccagc gcgcggatcc gaaacctgga
     1261 tggtgcgaag agctggaggt ttctggagtg acgccgacga tgagagatga aggcaatgac
     1321 acctgaaacc ttcagaaaac tcgcgctgtc acccccggag tcctccgaat ctgcgcatat
     1381 gaatcatcct gacttccggg ttggcaagag aatcttcgcg acttgatctt gccggcgctg
     1441 atggatgcgt ggcgaaacac cgctccggca gcgcgcgatt tcctaggcac ccgccggatt
     1501 gggggcgcgc cagacccgtt cgaagggcag gcgccaggcg tgcggcgcga tcaactggtg
     1561 aatcgcattc gggccccacg agcccggcgc atacgatcgc accggtggcg gcgcattcag
     1621 caagggcgcc gacacttccc atagacgctc gatgccttcg gcagtcgtga acagcgtgtg
     1681 atcgccgcgc atcgcatcga gaatcagccg ctcataggct tccagcacgt cgccgatcag
     1741 acccgtctca tgcatcgcaa actgcaggct gagtttgtcg aggcgcatgc ccggaccggg
     1801 gcgcttgccg tagaacgaca gcgatacctt ggacgcatcc gccagatcga aggtcagatg
     1861 atccgggccc tgttcgccga ttccggatcc aggcgggaac atgctcttgg gcggctcgcg
     1921 aaaagcgatc gagataatgc gctggccttc ggccaggcgc ttgccggtgc gcagataaaa
     1981 cgggacgccg gcccagcgcc aattgtcgat cgaacacctc agggcgatga aggtctcggt
     2041 gtccgagcgc ggatcgacgc cttgctccga tcgatagccg ctgtattgcc cgcggaccac
     2101 ctgatgtggc tcgatcggct ccatgcttcg aaagaccttg ttcttctcct cgctgattgg
     2161 tttgggatcg agcgccgtcg gaggttccat cgccatgaat gccagaatct ggaacagatg
     2221 cgtgaccacc atgtcgcgat aggcgccggt ctgctcatag aacccgacgc gcttgccgag
     2281 gcttaaggtt tccggcacgt cgatctgcac gtgattgatg aaatttctgt tccagatcgg
     2341 ctcgaacagg ccgtttgcaa agcggaacgc cagaatattc tgcgcgggct ccttgccgag
     2401 gaaatgatcg atgcggaaga tctgctcctc atcgaacact gcgtgcagat tggcattcag
     2461 tgccaccgcg ctggccaggt cggtgccgaa cggtttttcc atgatgatgc gcgagcgttc
     2521 aaccagtccg gcttcgccga gcatgcgcac cgccgacagt gccgcgttcg gcggcacgct
     2581 cagatagtgc agccgacggc tctcaccgct gaaggtcttc tcggcccgct ctaccgcggc
     2641 tttgagtgtc gatgcgcccg ccgatagcgg cacatagtcg agcgattgcg cgaacgccga
     2701 ccacgcctct tcggcaaatt tgcgggtcga gaagtcatcg agcgccttgc gggccacttt
     2761 ccggaagccc tcggtgtcga tgtcgtccag cgacacaccg atgatccggc acccggggat
     2821 gaaaccggcg tctgacagat ggaacaggcc cggcagcagt tttctctggg acaggtcgcc
     2881 ggtcgcgcca aacaacacca gcacctgggg atattgcgga ccgacgcctg cattgttctt
     2941 tgccgccatg cttcattcct ttgtgtgagc tgcgccagcg cggcgcagaa gttcagcatg
     3001 tatcacaaag ccgtgactgg atcacgatca gagcacacaa ccatcgcaaa aaccgtcaga
     3061 tccgtcacgc cccggggcgc gaccggccca gggtttcctc agaatatctg gctaccgcgc
     3121 ggccttggcc gcggcctttc gttcccggcg ccgcgcgtgc aggatggctt cggtgtagcc
     3181 attggcttgc tcgcgaccct tgaagatcag gtcacgggcc gcggcgaagg cgatgctgtg
     3241 atcatagtca gcactcatcg cccggtagcc cgggatcgcg gcattctgtt tgtcgaccac
     3301 gatggccatg cggcgcagcg cctgctcgac ctgagtacgg gtgcatatcc gatggtgcaa
     3361 ccagttggcg atgtgctgag acgagatacg cagtgttgcc cgatcctcca tcagcggcgt
     3421 gtcgtagtag tcgggaacct tggagcagcc cacgcccgaa tcgatccagc gcaccacata
     3481 gcctaagatg ctctgggtgt tgttgtggag ttcctcttcg atctcggctg cgctccagga
     3541 gggcttgtcc gccagcggtg gctgcaggat ttcatcgagc gaggcctgtg ggcgcgagcg
     3601 cagctgcgcc tggatcccga acacatcggc ctggtgatag tgaatggcat gcagcgtggc
     3661 agcggtcggt gagggcaccc acgcggtgtt ggctcccgcg cgcggatgcg caatcttggc
     3721 actgagcatc gccgccatca tgtccggcat cgcccacatc cctttgccga tctgcgcctt
     3781 gccgggcagg ccgcagcgca atccgatgtc cacgttctgc cgctcgtagg cgtccagcca
     3841 ttgggccttc ttcatgtcgt tcttgcgcat gaccgcgccg gcttccatcg aggtatgaat
     3901 ttcatcgccg gtgcggtcca gaaagccggt attgatgaac accacccggc gtcgcgccgt
     3961 gcgaatgcac tgctgcagat tgaccgacgt gcgtcgttcc tcgtccatga tgccgatctt
     4021 gagcgtgtcg cgcggcaacc cgagaagcgt ctccacacga gcgaacagtt catcggcgaa
     4081 ggcgacttcc ggtgcgccgt gcattttcgg tttgacgatg tagaccgaac cgcgccgcga
     4141 gttgcgcagc gggctgccgg acttcagatc atgcatcgcg atgagagagg tcacgaccgc
     4201 gtcgatcagc gtttccggaa cctccttgcc ctgcatcgtg atcgcatcac tcatcatgaa
     4261 gtgaccgaca ttgcgcacca gcatcaggct gcgaccgttc agtgtcagcg cgccaccgct
     4321 tggggtccgg tagctgcgat ccgggttcag gctgcggcgc accgacttgc cgcccttgtc
     4381 gaactcggcg ctaagggtac ctttcatcag tccgagccag ttgcgataga cgcgaacctt
     4441 gtcgtccgca tccaccgcag ccaccgagtc ctcgcaatcc tcgatcgtcg tgatcgccgc
     4501 ctcgagcacc acgtccgcca caccggcggc atcggtgcga ccgatcgcat gggagcgatc
     4561 gatctgaatc tcgacgtgca gcccgtgatg tttgagcagc accgcgctcg gcgcgcccgg
     4621 ctctgcctga aagccgacga gcgctccgcc gttgcgcagc gtcgcggtct tgccgtctgt
     4681 gaacttcaca ctcagaccgc ccgcagagac agcatacgcg atcgcctgtg cgtgcgagcc
     4741 ggcgttcagc ggaaaatgct cgtcgaggaa tgcgcgcacg taggcgatga ctttcgcgcc
     4801 gcgcttcggg ttatatgcgc cagcacgcgt cgccccctgg tcttcgggaa tgacgtcggt
     4861 gccgtagagc gcatcgtaca ggctgcccca acgcgcgttc gcggcgttga gcgcgtagcg
     4921 cgcgttgtcc accggcacga ccagctgcgg accggcgatg gtcgcgatct cgtcgtcgac
     4981 gagctgcgtc tcgatggtaa aggattcacc tgccggctcc agataaccga tctgctcgag
     5041 aaatccgcga taggcctgat ggtcgaactt cggtcccggg tgagcctggt gccactgatc
     5101 gatctgtgcc tgcagttgat cgcgacgctg cagtagctgc gcgttgcgcg gtgccagatc
     5161 gcgcatcagc tggtcaaacc ccttccagaa tgattccggg ttgatgccgg tgccgggcag
     5221 ggcttcctgg ttcacgaagt cgtacagcac ccgggcaacc tgcaattcac cctgggtcac
     5281 gcgatcgctc atgatttgtc cttgtggcgg cgaggaggcc tgcaattgcc ggccgcgaat
     5341 catagcaatt cgacgcttgc cagctcaaac cgccgcgatt catgcgccca cgcgctggcg
     5401 ccgggtctca tggccacggt tccaatggtg ccgtcacgac ggtcaaaagt cgtaacaaat
     5461 gaaactatac gatatactgc cggcctcatc ttcggcgctc gagccagatc cgcaaaggca
     5521 tcccatgagc cctgatatcc acgacaatcc tctcggcacc gacggattcg agttcgttga
     5581 gttcaccagt cccgatccga tcgcgctcgg cacgattttc gaggccatgg gtttcaccgc
     5641 ggtcgcgcga caccgctcga agaacgtcct gcgctacaag cagggcgata tcgatttcat
     5701 tctgaatatg gagccgagtg gccaggcggc ggcgtttcgc ggcgcgcacg gaccatcgat
     5761 caacgcgatg gcatttcgcg tgcgcgatgc cgccaaggca ctgcagctcg cgctggagcg
     5821 cggcgcgacc gaagtgaagg gtgcggtcgg gccgatggag ctgaacattc ccgccattca
     5881 gggcatcggc ggctcctatc tctatctggt cgatcgttac ggcgcgcacg agatctacga
     5941 cgtggacttc gtgccgattg cgggagccga cgcaatggag gccgtcggca gcaccggtct
     6001 tcgttacatc gatcatctga cgcacaatgt gcatcgcggc aacatgaaag tgtgggcgga
     6061 gttctacgag cgggtgttca atttccgtga gattcgctac ttcgacatcg aaggcaaggt
     6121 caccggcctg atttcgcggg cgatgaccag ccccgacggc aagatccgta tcccgctcaa
     6181 cgaaagccag gacgacaggt cgcagatcga ggaattccta cacgtctacc ggggcgaggg
     6241 cattcagcat gtcgcgctcg gcagcgacga cattttcgcg accgttgatc gcatgcgcgc
     6301 gcgcggtgtg caattccagg acacaccaga gagctactat gaggcgattg agacgcgcgt
     6361 gcccgggcat ggcgagaacc tcgaccgtct gcgcgaacgc tccatcctga tcgacggcgc
     6421 accaacacgc gggcaagggc tgttgctgca gatattcacc cagaacgtgg tcggaccgtg
     6481 cttctttgaa atcatccagc gcaagggcaa tgagggcttc ggcgagggca atttcaaagc
     6541 gctgttcgag tcgctcgagc tcgatcagat gcgccgtggc gtgttgtgag cgacacgctc
     6601 gcctacctga cgggcttcgc cagtcatttg gagagcgagt ccctgccggg ggcgctgccg
     6661 gcgcagaact cgccgcagcg ctgcccctac gggctgtatg ccgagcagat ctcaggtacc
     6721 gcgttcacgg ctccgcgtgc ggtcaatcgc cgttcctggc tgtatcgcat tcgtccggcc
     6781 gtgacgcatc gcccgttcca gaccctggac ggcgagcgca tgcacaacca gttctgcgat
     6841 tcgccggtgt cgcccaatca gctgcgctgg gatcccttgc cgatccccgg ggcgccgacc
     6901 gaattcgtcg atgggatcgt gacctacgcc ggtaccggtg agccggggac acaccagggc
     6961 tgcggcatcc atctctacag cgcaaaccgc tcgatgacac gccgtgcctt ctacgatgcg
     7021 gatggcgagc tgctgctatt gccacagcag gggcgagtgc tgctgtatac cgagttcggg
     7081 cggctgctgg tcgagccgct cgagttcgcc gtgatcccgc gtggcgtgcg cttccgggtc
     7141 gagctgctcg acggcgcggc gcgcggctat gtgtgcgaga actttggcgc gccgcttcgt
     7201 ctgccggaac tcggtccgat cggctccaac ggcctggcgt gcgaacggga ttttcacgtg
     7261 ccgacggcct ggtatgagga cagcgacggc gactatgagc tgctggccaa attcgacggc
     7321 aagctctgga gcgttacgct cgatcactcg ccgttcgatg tcgtggcctg gcgcggcaac
     7381 cacgcgccgt acaagtacga tctgcgccga ttcaacacca ttgggtcggt gagtttcgat
     7441 catcccgatc cgtcgatctt cacggtgctg cagtcggtgt ccgcttccgc cggtatcgac
     7501 gatctggcct tcgtaatctt tccgccgcgc tggctcgcga tggaacatac gttccggccg
     7561 ccgtggtttc atcgtaatgt cgccagcgag ttcatgggac tgatccgcgg cgcctacgat
     7621 gccaaagcgc aagggtttgt gccgggcgga gcatcgctgc acaattgcat gagcggtcac
     7681 ggcccggatg cgcagacctt cgagcgggct tcgcgcgccg acaccagcaa gccggagcac
     7741 gtaatggata ccatggcgtt catgttcgag acgcgcagcg tgatccggcc aacgcgcgcg
     7801 gcgctcgagt cccggttatt gcagcaaaac tactaccaat gctggcaggg cctgcaaaaa
     7861 cacttcactc catcgcagcg ttgaagcgtt gccgatgaca ttgaacgaga cacacgatcc
     7921 gcagcttcga agctgggtgg cgagcgccaa ccttgatgac agcgatttcc cgatccagaa
     7981 cctgccgttc ggagtcttcc gcgcgcaggc agacatcgcg ccgtgcggtg gcgtcgcgat
     8041 cggggatcag atcctgagtt tcggcgccgc tcaccgcgcc ggtgcattca gccccagcga
     8101 cgtcggcccg ctcgccgcgc gggccgctgc cgcggcggcc ggatcttcac tcaacgaatt
     8161 catggctctg gggcgcgaga gctggtcggc gctgcgcctg gcactgtcgc gcgcgctgcg
     8221 cgagggcgct acgcagcgcg cgatgctgca gacgtgtctg gttggtcagt cacgcgcgca
     8281 gctgctgttg cccgcgcaga tcggggacta cacggatttc tatacctcga tttatcatgc
     8341 gacggcagtc ggcaaactga tgcggccgga caatccgctg ttcccgaact accgatggat
     8401 cccgatcggc tatcacgggc gcggctcctc gatcggcgtt tccggtcagc ggtttcaccg
     8461 gccttgcggg caaactctcc cgcgcggcgc tgccgccccg gtgatcggtg ccagccagcg
     8521 gctcgactac gagctcgagc tcggagtctt cattggccag ggcaatgagt ccggcacggc
     8581 cattggcatc gaggatgcgg aggcacacgt ctttgggctg tgtttgctga atgactggtc
     8641 cgcgcgcgat ctgcagggct gggaagctca gccgctcggt ccgttcctgg cgaagaactt
     8701 tgccacgacg atttctccgt ggatcgtgac gctcgaagcc ctcgagccgt ttcgctcagc
     8761 gtggcagcgg ccagcgctcg atccgcagcc gctcccgtat ctggagggcg cgcacctgcg
     8821 tgagcgcggc gcgatcgaca ttgagctgga agtgctgctg cagtccgagc ggatgcgtga
     8881 gcgctcgcaa ccgcccacac gcgtgtccgg cagcaactac cgccacgcgt actggacgct
     8941 gtcgcagatg gtcgctcatc acagcgtcaa cggctgcaat ctgcagcctg gggatctgct
     9001 cggcaccggt actcagtcgg gacccgaccc ggagcaggcc ggctgtttgc tggagttgtc
     9061 gcagggtggc aagcgggcgg tgcggctgcc caatggtgaa gagcgacgct ttctggagga
     9121 cggtgatgag atcgttctgc gcgcctggtg cgagcgcgcg ggcgcgcgac ggattggttt
     9181 cggcgcggcg gtgggtcgag tcctgccggc gcacgagcgc tgatgtatcc gcagcgcatc
     9241 gtctgcttga ccgaggagcc gaccgaagtg ctgtatgcga tcggtgagca ggatcgcatc
     9301 gtgggtgtgt cgggattcac ggtgcgtccg gcgcgcgtgc ggcgcgaaaa gccgcgggtt
     9361 tcggccttca cgagcgcgcg catcgatcgc attctcgaac tcaaacccga tctggtcgtc
     9421 ggcttctccg atattcaggc cgacattgcg cgcgacctga ttcgtcacgg tgtggaagtg
     9481 tggatcagca atcaccgcag cgtgcaggga atcctcagct acatccgccg cctcggcgca
     9541 atggtgggcg cggcgcagaa aagcgaagcc tatgcgtgct cgctcgaggc gcgcttgtcg
     9601 cagctggcgc aggcgggcgc gcagctctcg cagcatccgc gggtctactt tgaagagtgg
     9661 gacaagccga tgatcagcgg catccgctgg gtgtcggagc tggtgggcat cgccggcggc
     9721 gtggacgtat ttccggagct cgcaggctgc tcgctggcga aggaccggat cctgtgcgat
     9781 tcgcaggagg tcgtgcgtcg ccgtccggac atcattctcg gctcctggtg cggcaaacgc
     9841 ttccgggccg aacatgtgcg ggcccgcagc ggctggagcg aagtgcccgc cgtgcgggac
     9901 aacgagctgc acgagatcaa gtcacctctg atcctgcagc cgggaccggc cgccctgacc
     9961 gatggtgtcg aggccatgca tcggatcttt gcgcgctggg cgcagcagcc tcgcccctga
    10021 tcccgccggc gctcgcttga agggtccgcg tgagggcctt aggcgagatt ttacgattca
    10081 ctaggaagta tcttacgata tatcttaaga tatattttag tgaatcgcgt caagcgctca
    10141 accacgtccc acggggctga aatcgcgcgc cacctccaca gctgcactaa ttgagaatca
    10201 ttctcagtga tagataggta ttggctgaag ttttcgtcac gcgcagtgac acggccgtga
    10261 caactcggcc tacgccaccg cctagcctgc gcagcgtgga actgtgtgat ttcaacgtgc
    10321 tgcactggcg tggcgcccgg gccccgaggg ctgctgtgcc gcagacccgt tcgggggttg
    10381 atcggtttct gatcgacacc tgccagcgtc gcgtggcggt ggtcagcggc cgtgaagcgc
    10441 tggatcaggc tcggctcgag tttccgtcgg acggcgcgat cgaattgttt gccggtgcgg
    10501 atgcctacgc cttcctgctg cgctttgcgt gcggcctgga gagcaagctg gtcgccgaga
    10561 ccgagatctt cggccagatc aaacaggcct ggcgcgaatt ctcgggagcg cgctcgccgc
    10621 tggcgcggca gctttccccg tggattcagc tgttgtttca ggatgcgaag gccgtgcgcg
    10681 cgcagtgtct ggccaagctc ggcagcgcgt cctacggttc tcaggtccgg cgcttgctcg
    10741 ctgaggatgc gaaaggcggc ccaacgctgc tgatcggtgc cggacagctg gcacagtcgg
    10801 tcgctccatg gctgaccggt tccgagctgt ggctgtggaa tcggacctcg gagcgagcgc
    10861 aggaactctc gcgcgaactg gccaagcgcg atccggcccg tccggttcgc gtgatcgaca
    10921 gcagcgccga cgccgagctg gccgcctggc gcgcagcgcg taatgtcatt ctgtgcgttc
    10981 ccgccgatga tcctcatgac agcgctcgtg tgcaggcgtg ggcgcagcga tccgaccggg
    11041 gcgggcgcgt ggttcatctg gggctgggtg cacagggcgc gacgccgtgg aatgacttgc
    11101 ccgaattggt cagtctcggg gccctatttg aaatgctgca ggcgcagacc gagctgcgcc
    11161 ggcggcagat tgagcgtgcg cgtcgcgcat gtctggagaa ggcgctgctg cgctcgctcg
    11221 gtgcgaattc ctcgcacccg cacagctggg aagacctggc cgcttttcac gcgatctgag
    11281 gcatgaactc gcgggtactg aagctcggca cccgccgctc cgcactcgca cgcgctcaaa
    11341 gcgccgcagt ggcgcgactg ctgcaacagc atcatccggc tctggcggtc gaactggtcg
    11401 gcatcgagac ccagggcgat cgcattctcg acaaaccgtt gtcgacggtg gatggaaagg
    11461 aattcttcac cgccgagatc gacgctgcgc tgctcagcgg cgcggttgat ctcaccgttc
    11521 attcctataa ggatctgagc ctcgagcgct cgacccgcct gctgctcgca gcggtaccca
    11581 agcgcgaacc gccgggcgat atcgcattgt tcgcacccga cgtccggcag agcttggcgg
    11641 caggcgaaga gattcgcgtc ggcagttcat cgccgcgtcg cgccagcttc gtgccggatt
    11701 ttctgcagcg tatgctgccg gccgacccga tgccggcgcg ggtacgcctg gtagagctgc
    11761 gcggcaatgt cgattcgcgc ttgcgccgac tgcacgagcc gcgcggctcg ccgcgtcacc
    11821 tggacgggat cgtgctcgca ttcgcgggcc tggcaagatt gtgggccgac agcgccggcc
    11881 agcagctgct gcgcgaactg ttgacgccgc tgccgcgcat gctcttgccg ctgtcggcct
    11941 gtccggcagc accggcgcaa ggggcactgg cgatcgagtg tcgcagcgac gatccggaca
    12001 ctgcgcgact gctggccagc atcgacgatt ccgcaacgcg tcgggccgtc gacgccgaac
    12061 gagcgctgct ggcacagcgc ggcggcggtt gccaccagcg cttcggtgca acacagattg
    12121 agctgtccgg ccttggagcg ctgctgtacc tgcgcgatgc cgatgaatcc gggcggccac
    12181 tgcccattcc gatggtgcgc tggacccccg agacgccgct gcccgctccg cacggtccgc
    12241 tgcgcgcctg ggacggcagt cgtgcggaca gtgcaccagc ggcggaaccg attccggagg
    12301 ccgtggcgcg ctgcgccgaa cgcctcagca gcacccgggc gctgttcgtc gcgcatcgac
    12361 gcgcgttgcc ggaagaattg accgcgagcg ttgatccggc cgcgcatgtg tgggtacccg
    12421 gcatcgagac ctggcaggcg ctcgccgagc gcggcatttg ggtggaagga tgcggcgaga
    12481 gcttaggatt tgcggcgctg gcgccaatgt tgacgcagcc gtcgttgcag ctgccagcgc
    12541 tcgatcaatg gaccgtgctg acgcacgccg aggccgccgg cggctggccg gcgaccgcgg
    12601 tggtcgcgac ctatcgccat gcaagttctt cgccagtcgg tgcagacccc gcgggtcctg
    12661 cgccggacgt gacccatgtc tactggcaca gcagtgcaca gttcgagcgc tggcgcgcga
    12721 gtgtcgcgcc gggcgtccac catgcgtgcg gaagtggacg cacgtccgaa catttgcggc
    12781 acgcagcggt gcaaaatctg cgtgtgtttc cgcaagtggc gctctggcgc caatggttgg
    12841 gattgtgact cgaagcgcgg gtgagggtgg actgagtgtc acgccgatgc ggcgcacctc
    12901 gtgggtcgcc gcggcggtgg cggtatgggt cacactgcag ctggtactgg tcatctggtg
    12961 ggccattctg atccagcgtc aggcgcagcg catcgcttcg ctcgaaacgc tcagcggcgc
    13021 cggtcacgag cagatcgccg cgcaggcctc gcgcacccac gtcatgctgg tgggcgaatc
    13081 gagcacgttc ctggcgctgc tggtggtcgt gacgctgcta ctggcgtggc tgtattggcg
    13141 tgagcatcag cgagcccgca gcatgcaggc attttttgcg tccgtgacgc atgagctgcg
    13201 cacgccgctc accagcattc gactgcaggc agaggccatt gccgaaggcg agcaccgcgc
    13261 gcagctcgcg cgtcggttgc tggaagactc cagtcgcctg gaatcccaga tcgacaagac
    13321 acttgaactg gcgcgcatcg aaggcggcgg gccgttgtcc gagcagttga ttccgctgca
    13381 tacctggatc gcacgctcgc tcgagggtat cggtgcggcg tttggcgatc gcctggatct
    13441 gcgcgcgcac gtggatggct cggtgccggc agtgtttgcc gatgcgggcg cgctgcagat
    13501 gattctgcgc aacctggtgg aaaactcggt gcgccattcc ggtgccacac cggtcagcgt
    13561 gcgcctgacc gcggcgcagc gcggcgacta cgtggtactc gagtatcagg acaacggcaa
    13621 cggcctgccg gggccttcac cgcgtttggg caagctgttt gcgcgcggcg tgaactcgag
    13681 cggcgccggc gtcgggctct atctggtacg ccgcctgatg cagcgcatgg gcggtcgcgc
    13741 caggttcaac actgcgcccg gagagggcta tcgctgcgag ctgtctttcc gggttggtgc
    13801 atgagcggcg ccacccgatt gctgctggtc gaggatgagc gcaacgtggc cgagaccctg
    13861 atggagcgac tgcgagccga gggctttcag gtgcagcgcg ccgattcggc agccagtgcg
    13921 cggcgcgcca ttgccgagtc ggaagtcgag atcgcgctgc ttgatgtcgg cctgcccgac
    13981 ggcaacgggt tcgaactcgc caagctgctg cgtgagcgcg ctccgggcac cgcgatcgtc
    14041 tttctgaccg ctcacgccac gcccgaggac cgcgtgcgcg ggctcgaact cggagcggat
    14101 gactatattg gcaagccgtt ccattttcgc gagctgttgc tgcggatcaa caacactctg
    14161 aagcgtgcgc aggagctgca gcgtgtgccc ggcgacattc gcggccaggt gcgcatcggt
    14221 cgcgcacagg tggacttcga gcgcttctcg gccaacgtcg gtggcgaaag cgtcgcgctc
    14281 acgcacaagg agtgtgcggt gctgcggctg ctggctgagc gcgtcggcaa agcggtcagc
    14341 cgcgatgaaa ttctggatcg cgcctggtcg gccgatgagt ttccgacttc gcgcacggta
    14401 gacaatttca tcgttcggct gcgaaagttg atcgagctca acgccgctga tccgcgcgtc
    14461 atccgcagca tccgcggcgt gggctatctg ttgaccgaaa tcaatcgtga gtgagcgatg
    14521 agcgaaacgc tgctgcaaca ggcgctcgcg cgccgtaacc atcaccggcc accggtgtgg
    14581 ttcatgcgcc aggccggtcg atatcatcga cactaccagc ggttgcgcgc gcggcacgag
    14641 ttcatcgacc tgtgcaagcg gccggagctg gcggtcgaag taacgctcgg cccgatggag
    14701 gagttcggct tcgatgccgc aatcctgttc tccgatctgc tgttcccgct cgaagcgatg
    14761 gggatgggcc tgagctacga gccgggtccg cgacttggct ggcacttgcg cagcgccgcc
    14821 gacctgacgc gcctgcaggg cggggccgat cgagccgctc acatgagctt tcaggccgag
    14881 gccctctcgc agctgcgcgc gcggctggat ccgtaccgtg ctctgatcgg tttcgtcggc
    14941 ggtccgttca cgctgtttgc ctatgcggtc gccggctcac acgaagggtt tgcgcgcgat
    15001 acgctcgacg accatgacgg cacgctgtat cgcggcttca tccagaaact gcagccgctg
    15061 ctggcggcga acatggtctt gcaggttcgc ggcggcgccg attgcattgc gatcttcgat
    15121 accgcggccg gcacgctcgg cgccgaaggc ttcgcccgac aggtggcgcc cgtattgcgc
    15181 ggcctggtga gcgagtttcg cgcccagtgt ccgcagacac cgctgatcta ttactcgcgc
    15241 gacaccggac cggcgcattg ggacgcgctg cgcgatatcg attttcagtg cctgggcatc
    15301 gactggcgac atgatctggt ggatgtgctc aatacctacg cggatcgctg ggcgctgcag
    15361 ggaaacatcg atccgcagtg gttgttgttg tcgcgcgaac agctcgaggc gcgcctgcgt
    15421 aacctgtttg cgcgcgtact gctgttgccg gcggcacaac gtgcggcctg ggtatgcgga
    15481 ctgggtcacg gggtcctgca gcacacaccc gaagacaatg tccggctggc gctgagcatt
    15541 cagcgcgaga tgttcacatg agcgtcgcct tgggcgatcc gctgatcgcc aaatacgaca
    15601 tcgcaagtcc gcgctatacc agctatccga cggtccccta ctgggacagt gcgccgacgg
    15661 ccgatcaatg gatcgagcat atcggccgcg cgctgcaggc cgccgaggcc tcgctggggc
    15721 tgtacgtgca cattccgttc tgccgcacct tgtgtacctt ctgcggttgc aacacgcgca
    15781 tcacgcgcag ccacagcatc gtcggaccgt atattcaggc gctgctggcg gaactggact
    15841 tgtatcggca gcgtttgggg tgctccgagc tcgaatttgg cgagctgcag ctggggggcg
    15901 ggacgccgac gtttctgacg gtcgaggagc tggaggcgtt gctatcggct ctgtaccagc
    15961 gcgctcggcc gcgccgctcg gcgatgatcg catccgtcga ggttgacccc cgagtcaccg
    16021 atcccacgca gctggcgctg ctggcacgct acggcttcag atccttgagc ctgggggtgc
    16081 aggacttcga tccgcgcgtg caggacatcg tcaaccgcgt gcaaagcgag gcccaggtgc
    16141 agcagttaac cgaggccgcg cgcgtgcttg ggttcgagag tgtcagttac gatctgatct
    16201 acggtttgcc gctgcagacc cgcaccagta tcgatgcgac catgaatgcg ctggagcgcc
    16261 tcaggcccga tcgggttgca ctctatgcct atgcgcatgt tccttggatc aagcccgggc
    16321 agcgtcgctt taccgatagc gatctgccgg agtcggaaga gaaacgtgaa ctctatgaac
    16381 tggggcgcga gcgcctgaca gcgcagggct atcgcgaaat cgggctggat cactttgcgc
    16441 tggcaagcga cggcctgtgg caggcgcagc aggcgggccg cttgcatcgc aatttcatgg
    16501 ggtacaccag cgcctactcg cggccgatca tcgggctggg tgtctcctcg atcggcgatg
    16561 ccggcgatgc cttcgcacag aatgaaaaag acttgcagcg ctatcacgag cgcatcgcca
    16621 acggcgaact gccactgcaa cgcggacacg tgctcgatac cgaggatcgg atcttgcgcc
    16681 gacacattct gaggctgatg acacagcgca ccacgcgctg ggatcaggcc gccgagtaca
    16741 cgccgttcct tgcgacgatc ggcgcccggc tggcggagcc ggcggccgat gaactggtgc
    16801 tgctggagga gaagggatgt cggatcaccg agcgcggccg gccattcctg cgcaacatct
    16861 gtatggcgtt cgatgccagg ctcgcgcgcc gtatgcccga gaaactgccg ttcaggtaag
    16921 gcggtgtgac gccggagttg gcgctagaaa ggtttcatca ccgcgagcag gatgatcggc
    16981 accacgatca gcagggcgga ctcattcagc acacgcagca cccagggctg cggcatcggc
    17041 gccccgcgcg tgatgcgcat gagcagtgcg cggcaggaga ggtgatacgc cagcagtgcg
    17101 agtacgaaca ccagcttgac catcagccag cgcccggtgt catgaaagcc cagccacagc
    17161 cacaacccga acaggattgc gagcagcccc atcatcgtca tgaacccaaa cagtcgccgt
    17221 gccatgatgg ccagtcggcg cacgtcttcg cccgcggcct ggccttccgc gtagtgcacg
    17281 aggatacgcg gcagatagaa caagcccgcg agccaggcca tgacggcaat gacgtgcaac
    17341 gatttgaccc agagcatgac aacactatag atgaatcagg cg
//
LOCUS       DATMRM010000002         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2790381, whole genome shotgun sequence.
ACCESSION   DATMRM010000002 DATMRM010000000
VERSION     DATMRM010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2790381"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1494)
                     /locus_tag="VNZ06_00085"
     CDS             complement(97..1494)
                     /locus_tag="VNZ06_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW19175.1"
                     /translation="MSARALVVDDSRSARAFLTRELERHELSVDGVESAEQAIDYLTR
                     QRPDVIFMDHLMPGMDGFQAVQAIKNNPRTATIPIMMYTSQEGELYLSQARALGAIGV
                     LPKQIKHADVSKALEQLHLIRPPGTATAGANAAAAPAADSFAPSEKPIPAVQLSGDVH
                     EIGRERRGPRRPDLPALPPNLRSILDAMFAHHNNEMRRFVVDHMESQADRIVGDMRLL
                     LQDQPTSAAQFPRRPQPVRWGLWLGLAGVAVASLFAVEWFHERTASEQLAAQLAQSQQ
                     QLQTVQQNLSSLQAADAAAAASSGAPLGDSAGAGGIDQTSSVEPVPFGEMPLSGARLD
                     QVASLLSRLNSQGFQGVLQVRTIPGRFCMANGLSGQPVLAPDTTPYSKCEQIGNPRED
                     NGTAAQRESVAFANMISTAQRNSAGRIEVQISAGNSDEIQTPYPPVSDALLAGEWNRV
                     AAANNRVELRWQARH"
     gene            complement(1596..2777)
                     /locus_tag="VNZ06_00090"
     CDS             complement(1596..2777)
                     /locus_tag="VNZ06_00090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW19176.1"
                     /translation="MRVLFISDVYFPRVNGVSTSIRTFRADLMTLGVESTLVAPEYDH
                     SAEEPDVNVIRVASGRVPGDPEDRRMKSAAVGRALAPLASQNFDLVHIHTPFIAHYAG
                     VRFARRRGIPVIATYHTFFEEYLHHYVPLLPRPIGRVLARSFTRSQCAQVAAIIAPSE
                     PMRALLLEYGVKSRVEVIPTGLPADRYVQGDGNRFRETYRIPKDRPLLLYVGRVAHEK
                     NIDFLLLAFTELRRTRPETMFAIAGEGPALSHLQAQVARQGLSAHVFFIGYLAREGEL
                     ANCYAAADVFVFASRTETQGLVLLEALAQGRPVVSTAHLGTRSILQAGCGARVAPERP
                     EAFARAIADVLEDPVRAERLSEQAHSYAQTWASSRMAARLSTLYHDLIKSATAPAIAA
                     A"
     gene            complement(2795..>3338)
                     /locus_tag="VNZ06_00095"
     CDS             complement(2795..>3338)
                     /locus_tag="VNZ06_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWW19177.1"
                     /translation="MNRAQIIMARFDVAEYRLCRHLNRGAASRSLRFVFRLASRLGDG
                     VVWYGLVGVLPLLYGRAAMRPAISMAITGITGLLIYKWLKHALVRERPFIRHPGITLA
                     MPPLDRYSFPSGHTLHAVAFTWQAVAFFPELAWVLIPIASLIAASRVVLGLHYPSDVL
                     AGGAIGAGLATTAIFAQNFV"
BASE COUNT          638 a         1078 c         1057 g          565 t
ORIGIN      
        1 cgccggtgca ttaccgaagc gccgcgccgt tgccagcacc gacgaactgc ccctgcgctg
       61 acggcatcct caccggcacg tctgtcccgg accgtgttag tgacgcgcct gccagcgcag
      121 ttcgacgcga ttgttcgcag cggcgacgcg attccactcg ccagccagca acgcatcgct
      181 gacgggtgga tagggcgtct ggatttcatc ggaattgcca gcgctgatct gtacttcgat
      241 cctgccggca gaatttctct gcgccgtcga aatcatgttg gcgaacgcga ccgactcgcg
      301 ctgggcggcg gttccattgt cttctcgagg atttccaatc tgctcgcact ttgaataggg
      361 cgttgtatcc ggtgccaaca cgggctggcc agacaaaccg ttcgccatgc agaagcgtcc
      421 gggaatggtg cgtacctgaa gcacaccctg aaagccctga ctgttcaggc gggagaggag
      481 tgatgcaacc tgatccaatc gagcgccgga gagtggcatc tcgccgaacg ggacgggctc
      541 gaccgaggaa gtctgatcga tcccgcccgc gccggcacta tcgcccagcg gtgcgccgga
      601 gctggccgcc gccgcagcat ccgccgcctg cagcgaagac agattctgct gaacggtctg
      661 cagctgctgc tgcgattgtg cgagctgggc ggcaagctgc tcggaggcgg tgcgctcgtg
      721 aaaccactcc acggcaaaca gcgacgccac cgctacgccg gccaatccga gccacagacc
      781 ccatcgaacc ggttgtgggc gtcgcggaaa ctgcgcggca ctggtcggct gatcctgcag
      841 caacagccgc atatcaccga cgatgcggtc cgcctgcgat tccatgtgat cgaccacgaa
      901 tcggcgcatc tcgttgttgt gatgcgcgaa catcgcgtcg aggatggaac gcagattcgg
      961 cggcagcgct ggcaggtcgg gccgccgcgg tccgcgtcgc tcgcgcccga tttcgtgcac
     1021 gtcgccagac agttgcacag caggtatcgg cttctcgctc ggcgcgaagc tgtcagcggc
     1081 cggcgcggcg gcggcgttcg caccggcggt cgcggttccg ggcggacgaa taaggtgcaa
     1141 ttgctcgagc gccttcgaca catcggcatg cttgatctgc ttcggcagca cgccgatcgc
     1201 gccgagcgcg cgcgcctggc tcaggtacag ctccccttcc tgagaggtgt acatcatgat
     1261 cgggatcgtg gcagtgcgcg ggttgttctt gatcgcctgc acggcctgaa acccgtccat
     1321 gcccggcatc aggtgatcca tgaagatgac atcgggacgc tgccgcgtca ggtagtcgat
     1381 tgcctgctcg gccgactcca cgccatcgac cgacagttcg tgccgttcca attcgcgcgt
     1441 tagaaatgcc cgggccgatc tcgagtcatc gaccaccagc gctcgtgccg acattgagaa
     1501 ttcgctcgta gcggtgctgg cccgatctta acgcatgcgc ccgccggtcg cgctcttgca
     1561 caggtcgcaa caatgaacat gtctcaactc tgcgactagg cagcggcgat cgcgggtgca
     1621 gtcgcggact tgatcaggtc gtggtagaga gttgacagcc ttgcggccat gcggctggaa
     1681 gcccacgtct gggcatagct atgtgcctgt tccgacagcc gctcggcccg caccggatct
     1741 tccagtacat cggcaatcgc acgcgcgaac gcttcgggac gctcgggggc aacgcgcgcg
     1801 ccacagcctg cctgcaggat cgacctcgta ccaagatgcg ctgtggaaac cactggccta
     1861 ccctgcgcca gcgcctccag caggaccagg ccctgggttt ccgttcgaga ggcgaacaca
     1921 aacacgtcag cggccgcgta acagttggcg agttccccct cacgtgccag gtacccgatg
     1981 aagaacacgt gcgcactgag tccctgccgc gcgacctgcg cttgcagatg gctcagggcc
     2041 ggcccttcac cagcgattgc gaacatggtt tccggccgtg tgcgtcgcaa ctcggtgaac
     2101 gccagcagca ggaagtcgat attcttctcg tgtgcgaccc gaccgacata cagcaacaac
     2161 ggccgatcct tcggaattcg gtaagtctcg cgaaaccggt tgccgtcgcc ctgcacatag
     2221 cgatccgctg gcagccccgt ggggatgacc tcgacgcggc tcttcactcc gtactcgagc
     2281 agcagcgcgc gcatcggctc cgacggcgca atgatcgccg cgacctgcgc acactgcgat
     2341 cgcgtgaaac tgcgtgccag cacccgtccg atcggccgcg gcaacagcgg cacgtaatga
     2401 tgcaaatatt cctcgaagaa cgtgtgatag gtcgcgatca ccgggatgcc gcgccggcgt
     2461 gcgaaacgca ctcccgcata gtgggcgatg aacggcgtgt ggatgtgcac caggtcgaaa
     2521 ttctgcgatg ccagtggcgc gagcgcgcgg cccacggccg ccgacttcat gcggcggtct
     2581 tcggggtccc cgggaacgcg cccggacgcc acccgtatca cattcacgtc gggctcttcg
     2641 gcgctgtgat cgtactcggg agccaccagc gtcgactcca cgccgagggt catcagatca
     2701 gcacgaaacg tgcggatcga cgttgaaacc ccattgacgc gtgggaagta gacgtcggag
     2761 atgaaaagga cccgcatagt ggcgggtgcg aagcttaaac gaaattctgc gcgaagatag
     2821 cggtggtagc cagtcccgct ccaatcgcac cgccggccag cacgtcactc ggatagtgca
     2881 atcccagcac gacgcgcgag gcggcaatca ggctcgcgat cggaatcagc acccacgcaa
     2941 gttccgggaa gaaagcgacc gcctgccagg tgaacgcgac cgcgtgcaag gtgtgaccgg
     3001 acggaaagct gtagcggtcg agcggaggca tcgccagggt gatgcccggg tgacggatga
     3061 acggccgctc gcgcaccagt gcgtgcttga gccacttata gatgagcagt ccggtgatgc
     3121 cggtgatcgc catgctgatc gctgggcgca tggcggcgcg cccatacagc aacggcagca
     3181 caccgaccag cccgtaccag acgacgccgt cgcccagacg actggcaagg cggaacacga
     3241 atcgaagaga gcgagaagct gcgccgcggt tgagatgccg gcacagccgg tattcagcga
     3301 catcgaatcg agccatgatg atctgggcgc ggttcatg
//
LOCUS       DATMRM010000003         4552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1551123, whole genome shotgun sequence.
ACCESSION   DATMRM010000003 DATMRM010000000
VERSION     DATMRM010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551123"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VNZ06_00100"
     CDS             <1..422
                     /locus_tag="VNZ06_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19178.1"
                     /translation="GRVKLRGAEVQATGSPNADWQWRATYAYLDNYDASTSLEQTEYS
                     RHSGSLSISRTYGEGWRWSVADYGATGNGIGQRGYERADATLGKAWSVYGKPISLDVT
                     GSYYFTLTSSYYRDVDSIGLSSYNNRFRILATVRVQL"
     gene            779..1435
                     /locus_tag="VNZ06_00105"
     CDS             779..1435
                     /locus_tag="VNZ06_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19179.1"
                     /translation="MTREEYRNIVGTHTDISAVYAEVSPNVQLQLVKQLYQRPVRVAV
                     MLSEETDDLRGLFASAAAQAGLTLQIIRATRSSDPGRAVATAGNFDALVIIPDPSVLS
                     AMSFRTILEATYRRGEGVIGFSAGQVGAGTLAAPFASLSDILAQLDEMLGAYRTTGDL
                     PAPTYPRYWSVAINESVAHSLDLVVTDEARQMASRPNRADEAPPGLPSHASGDAPRSP
                     "
     gene            1432..2952
                     /locus_tag="VNZ06_00110"
     CDS             1432..2952
                     /locus_tag="VNZ06_00110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW19180.1"
                     /translation="MKVRSPGLDNIWIRFAIPVVIASSVMLVFSIVMLSHTFQRDAND
                     DVTQRARLLMMAVEASTQFDVMSGNREQLQRTIEHAMREEPIVARIQIFDERHQIMAS
                     ASAENAEQAVAQFSTPIRTELSTVDLFNSDTEPHIGDAPPSHASAAAGRVIGFVEVRV
                     RSKPIIAARMRGSLYGIVLTLLAGFVSVAVGLAVGVRFRQQLRTLIHALRQIGSGDLS
                     VELPGKSPGEIGAMERTINQMARELDHTRGELEARVRERTDELQKAIERLAASDQQRQ
                     RLIAGQTAMLEEERRRISVEMHDHINSNLALIQLLSNDLQEQSQRLVDAHVAPVEGAR
                     MLEAATRIGDMTQKLYSTARGIVRTLRPESLDVLGLPRTVEELIHQFNAAGGHCLVQF
                     TESPNIPPLRGPVAVTAYRVIQEALSNIVKHANAVQADVSLLMNSEVRELQLVVTDNG
                     CGFDTRNIGAAGIGLASMRERVEGAGGQMLIRSGRTGTTIEVTLPLSDAEPTTNPR"
     gene            complement(3531..4259)
                     /locus_tag="VNZ06_00115"
     CDS             complement(3531..4259)
                     /locus_tag="VNZ06_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HWW19181.1"
                     /translation="MCGRYLTKDQRALERELFLNIIRWPQWAGSYNIAPTQLAPVVLD
                     TPDGHVCELMRFGLVPHFAHGEPPKYSTINARIENLTQAPSWRGPWKRSQRCLVLASG
                     FYEWHVLSDGKTKQPHLVRPQDQQSFAFAGLWDASKTADGTVTHSFAIVTLPASPLMA
                     QIHNAKKREPAMLTREDCVIWLGSSAEQAFSVLKQYPDELLLAYPVSHRVNSPKNNDA
                     RLMEKERAMTARPEDTAQNSLFVI"
BASE COUNT          930 a         1340 c         1331 g          951 t
ORIGIN      
        1 atggtcgcgt aaaactgcgc ggtgctgaag tgcaagcgac aggaagtccc aatgccgatt
       61 ggcagtggcg ggccacatac gcctacctgg acaattacga cgccagcaca tctcttgaac
      121 agaccgagta ttcgcgccac agcggctcat tgtcgatttc gcgcacatat ggagagggct
      181 ggcgctggtc ggtcgccgac tacggggcca ctgggaacgg gatcggacaa cgcggctacg
      241 aacgtgccga cgcgactcta gggaaggctt ggtccgtcta tgggaagcca atatccttgg
      301 atgtgaccgg gagctactac ttcacgctga ccagttcgta ctaccgggac gtggactcga
      361 tcggtctgag ttcatataac aatcgcttta gaatccttgc cacagttagg gtgcaactct
      421 aggcatctgc tgatgcaacg agtcgcgcgt gcctacaata atcatctggt cttgctccaa
      481 gctccataaa tacgcgtcca tcgtcctggg cgctgtcctc acgatctgta ttctggggtc
      541 ggccgctggc gctgccgagc acgctcgtgc cgcccggccg ctgtgtatcg ttctgggaag
      601 cccgagcacc caaactgagc agttgcagcc ggcattaacc tcacggtatc ccgccgcggc
      661 ggtcgtcaca accgcctcaa tgtgcccgga tggagcccaa ctgacgctca gcgtggggcc
      721 tgcggcacta cgcgacgtga cgctaagcgc tgcgcattac ccgacgattt ctttgttcgt
      781 gacgcgtgag gagtatcgga acatcgtcgg aacgcacacc gacatcagcg ctgtttatgc
      841 cgaggttagc cctaacgtcc agctccagtt agtcaaacag ctctatcagc ggcctgtgcg
      901 tgtcgccgtg atgttgagcg aagagacgga cgacctgcgc gggctctttg cttctgcggc
      961 cgcgcaggct gggttgacac tgcaaatcat tcgcgccacg cggtcctcgg acccgggacg
     1021 cgcggtcgcc acggccggga attttgatgc cctggttatc attccagatc cgagcgtgct
     1081 ctctgccatg agcttccgca ccattctcga ggcgacgtac cgccgcggcg agggcgtcat
     1141 cgggttttcc gccggtcaag ttggcgccgg caccttggcg gcgccgttcg cctccctgtc
     1201 cgacatcctg gcgcagttgg acgagatgct gggcgcttat cgcacaacgg gcgatttgcc
     1261 agcgccaaca tatccgcgct actggagcgt ggcgatcaac gaatctgtcg cccactccct
     1321 tgatctggtc gtcacggacg aagcgcggca aatggcctct cggcctaata gggccgatga
     1381 ggccccgccc ggcctacctt cccatgcgag cggcgatgcg ccgaggtcgc catgaaagtg
     1441 cgctctcccg gtctcgacaa catctggatc cggtttgcga taccggtcgt gattgcttcc
     1501 tcagtcatgc tggtattcag catcgtcatg ctatcgcaca ccttccaacg tgatgcgaac
     1561 gacgatgtga cacaacgcgc gcgtctcttg atgatggccg tggaagcatc gacgcagttc
     1621 gatgtcatga gtggcaatcg cgagcagcta cagcgcacga tcgagcatgc catgcgcgag
     1681 gagccaatcg ttgctcggat acagatattc gacgaacgcc accagatcat ggcgtccgca
     1741 tcagcggaaa atgcggagca agcggtggcc cagttcagca cccccatccg tactgaatta
     1801 tccaccgtag atctcttcaa ttccgacacc gagcctcata taggcgacgc gccgccgtcg
     1861 cacgcttccg ctgccgccgg tcgagtgatc gggtttgtcg aggtacgcgt gcgctcgaag
     1921 ccgatcattg cggcccgtat gcgcggctcg ttgtacggca tcgtgttgac gcttctggct
     1981 ggcttcgtca gcgtggccgt cggtctggca gtgggagtgc gattccgcca gcaactgcgc
     2041 acgctcattc acgcacttcg ccaaattggt agcggagact tgtcggtgga gttgccaggt
     2101 aaatcgccgg gagagatcgg ggcgatggag cgcacgatta accagatggc gcgcgaactg
     2161 gatcacacac gcggggaact ggaagctcgc gtccgagaac gaaccgacga acttcagaaa
     2221 gccatcgagc gacttgccgc gagcgaccag cagcgccaga ggctgatcgc ggggcagacc
     2281 gcgatgctcg aggaggagcg gcgacgtatc tccgtggaga tgcacgacca tatcaacagc
     2341 aacctggcac tcattcagct gctgtccaat gacttacagg agcagtccca gcgtttagtc
     2401 gatgcgcacg tcgcgcccgt tgagggtgcg cgcatgcttg aagcagctac gcgaatcggc
     2461 gatatgacac aaaagctcta ctccaccgct cgaggaatcg tacgcacgct acggccagaa
     2521 agcctggatg tcctggggtt gccgcgcact gttgaggagc tgattcacca attcaacgcc
     2581 gctgggggcc attgcctcgt gcagttcacg gaaagtccga atattccgcc gctaagggga
     2641 ccagtggctg ttactgcgta ccgcgtgatt caggaggctc tatccaacat agtcaagcac
     2701 gcaaacgccg tgcaagcgga cgtctcgttg ctcatgaaca gcgaagtgag ggagctacaa
     2761 ttagtggtca cggataatgg ctgcggattt gatacccgaa atatcggcgc tgccggcatt
     2821 gggttggcga gcatgcgtga gcgcgtggag ggtgccggcg gacaaatgct gattcgttcg
     2881 ggccgaacgg gcacgacgat cgaagtgacc ctgcctctga gcgatgccga gccaactact
     2941 aaccctcggt gagtgattgg tgccattttt cccacggatc tggtagctac agactccagc
     3001 gcaggaaagc tacccgccgg agttccaaaa cactgagtaa tgcattggat agctcaatcc
     3061 gagaaatcgt gctggcctca ctggacccgc atcgtatcag tgccatttcg gttcccgctg
     3121 atgcgacatc ggtcctacgt cgatccgagc gcccctagct ccgcacttca cgcagcgtag
     3181 gcgcttgctc agagcctcca gcgtgcattc ccagccgacc ctggacgcga gattggcggg
     3241 atcgacgatg cgtttattcc cacagctcag gcagccgatg tggagcaggt agttgccgaa
     3301 ctcatcgcaa agcttcacca cgtggagttg acgacgcata ctgattattg cccgggtcgt
     3361 ttatcggatc gaccgggtgg catccggaag cgcctttcct ctttgatcac ggcgtcggct
     3421 taggagatac gaagctagaa aggcccgact atcgccgtgg attactcagt gcgtggaagt
     3481 aaacatccac aatctttggc caggactcgc ggaagcctcg tgcacacatc ttaaatcacg
     3541 aacagcgaat tttgagccgt atcttcaggc cgcgcggtca tcgcccgctc cttttccatc
     3601 aatcgcgcgt cgttgttttt ggggctgttg acgcgatggc tcaccggata cgcaagcaga
     3661 agctcatcag gatattgctt caggaccgag aacgcttgct cggcactgct gcccagccat
     3721 attacgcaat cttcgcgcgt cagcattgcg ggttcgcgtt tcttagcgtt gtggatctga
     3781 gccatcagtg gggaggcggg caatgtgacg attgcgaagc tgtgcgtgac cgtaccatcg
     3841 gcggtcttgg atgcgtccca cagtccggcg aaggcaaagg attgttggtc ctgtgggcgc
     3901 acgaggtggg gctgcttcgt tttaccatca ctcagaacgt gccactcgta gaagcccgag
     3961 gccagaacga ggcaacgctg actccgcttc cagggaccac gccagctcgg agcctgcgtc
     4021 aggttttcaa ttcgtgcgtt gatggtgctg tacttcggtg gctcgccatg tgcgaagtgc
     4081 ggcacgagac caaatcgcat gagctcacac acatgaccgt cgggcgtgtc cagcacgaca
     4141 ggtgcaagct gcgtcggtgc aatattgtag ctaccggccc attggggcca gcggatgata
     4201 ttaagaaaca gttcgcgctc taacgcgcgt tgatcctttg tgagataccg gccgcacata
     4261 caacctcagc ttatctacta ttagcaggtt tacccgagcc gtcacgcctt ccgctcgggc
     4321 tattccgcac gctgaactgc ctacacgaaa cgaacatcac accgcgctca cagcaggtga
     4381 agcatgcggt tttgtaatag ccgcttcccc tcgggaccac gacttcctcg aattgactcc
     4441 cgcgcagatt ctgcgttgga agcgccctgc tccagatact gtatatctga ccagtatgtg
     4501 tcaagtttga gtatgaagta ggcttaggct tctttgagac atatccttaa tt
//
LOCUS       DATMRM010000004         5379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1670, whole genome shotgun sequence.
ACCESSION   DATMRM010000004 DATMRM010000000
VERSION     DATMRM010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1670"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1660)
                     /locus_tag="VNZ06_00120"
     CDS             complement(<1..1660)
                     /locus_tag="VNZ06_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19182.1"
                     /translation="MSQCPGRSTAAAIAASTGISSAATAAVLLGGLLSYALPSQAQQS
                     APTQAAQPQSPPQTCYRDDAGRIVTRRRPGYVEVPCPPPGTRTVQPGVGAPTSQPAPG
                     SNGANAGETVAAEVTPSVVSPVPRPAMQDFPQSVAVPDRWRIVDALGYPKNYFDPYNQ
                     NTLKADKPVFGGNWFFDLGLISDTVYQIQNVVTPVGGVSTANAGVNGVFGGTRQQVFS
                     QSAEADLTLFRGDTVFKPPDYQFKVTGVFNYNYLKTEDLTAVNIDPRQGTNRSDHFTG
                     LQAAFAEMRLREVSEKFDFDSIRVGIQPFSTDFRGFLFQDDQLGVRLFGTRNNNLYQY
                     NIAYFRLIEKDTNSGLNDIGAPLRKDDVFLFNLYSQDNPVQGFTSQVTVVYNRDREGD
                     EVHFNTDGFIVRPAALGLERARDYDVIYLGYNGDGHFGSWTVTTSFYWALGTDRQGSF
                     IAQNERINAQFFALELSHDFDWIRPRLSFLYGSGDRNPYSKNETGFDAIRDNPQFAGA
                     DSSYWIRQAVPLVGGGGVALAGGNNILNNLRSSQDEGQSNFANPG"
     gene            complement(1657..3390)
                     /locus_tag="VNZ06_00125"
     CDS             complement(1657..3390)
                     /locus_tag="VNZ06_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWW19183.1"
                     /translation="MDALLRQLKDGPDGLVEYHDTELSADALTIGSSANCSIQLLGEA
                     VGAQHALIRSSGGTVTLTCRAGRRVQLNGKPVSTSTLKLGDKLEIAGHQLQLVEPPMG
                     FELALEIRPDPNVSASSFERAFRTDLSETWLTKRRAAWGLLLLALLGAFLMPLASIYL
                     HRGGGGTPAGIPDDTFWTAGPLSPAHQLATGQRCDGCHETLFVHVRDSACRECHKSVG
                     DHVIPAHLAQTKLGPQQPCAQCHEEHNPSSANVIIRSNALCTDCHASSERSFGPIKVT
                     AVTGFSRSEHPAFTVDLLKPAFSAPGTVAELKWSTVRAPIAQAAEQSNLKFSHAQHLE
                     PSRVRRPGDSAPLTCADCHTLGADGEHFIPVTMLRSCITCHELTFDPSAPKRSLPHGK
                     PRDAMMLIEDFYARQVVDPHPPSAPPFQRRRLPDQASVDYEDTSQICRAATFDCAMQK
                     ARAQIDNQFTRRGCVSCHVVLDTKAADILERYQVTPIRLTFDYFPDAHFSHRSHEVQK
                     GKAGDEACLACHSAVTTDSSTVVMIPDLPKCLECHTPDSERDKVQLQCISCHTYHPKL
                     IIAEDREARRE"
     gene            complement(3394..4281)
                     /locus_tag="VNZ06_00130"
     CDS             complement(3394..4281)
                     /locus_tag="VNZ06_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19184.1"
                     /translation="MIHEGILRFRASRYLWWALGLTIGCFLLYYTQGGNHPPSGGTWQ
                     GYTLGTIGALLILWLTMLGIRKRSYASSLGSVQGWTSAHVYLGLALIFVATLHSALRF
                     GWNVHMLAYVLMCAVIGSGIFGVYTYLANPRQLAQNSGGRPRSELFAELFELDKKGRD
                     LAQACDPAVSVAVKSSIERTTIGGGVYAQLFSIDRSWFLRTEREGTGSRLASNVDQQA
                     VVEFVANRVSRTSKRGEPATLQALVAVLSRRQAVLRRIRRDIRVQGWLRLWLYVHVPL
                     TFALLAALIAHILTTFMYW"
     gene            complement(4278..>5379)
                     /locus_tag="VNZ06_00135"
     CDS             complement(4278..>5379)
                     /locus_tag="VNZ06_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWW19185.1"
                     /translation="GAGCVLVETPRRTMLKLMNSNAEVRNGIDWIFVVRELKSQFAPR
                     APLRDLREIGTKVSVRRFKAGEIIYKEGSTADSLFIVRSGAVTQTRLREGRELLVAQI
                     AAGQLLGEMALMGDPVRRETARATLALEMIEVRRAEFLQLTQRRDALIDPLQRAASER
                     VTENARMEVRPESGSLMNFLMDEGLGEATDVLVIDESLCVGCDNCEKACAETHHGISR
                     LDRKRGASYAQVHIPISCRHCEQPHCMKDCPPNAIKRSPTGEVYIDDTCIGCGNCQVN
                     CPYGVIRMEYEAPKKPSLLQWLLLGRGPGVGEEVHYHPTDAAKAKGKKAVKCDACVSV
                     PTGPACVKACPTGAAMRISPSQYADLVEERRR"
BASE COUNT          891 a         1720 c         1796 g          972 t
ORIGIN      
        1 tgccggggtt ggcgaagttg gactggccct catcctgcga ggagcgcagg ttgttcagga
       61 tgttgttgcc accggccaat gccacaccgc ccccgcccac cagcggcacg gcctgacgga
      121 tccagtaact ggagtccgcg cccgcgaact gcggattgtc gcgaatcgca tcgaatccgg
      181 tttcgttctt gctgtacggg ttgcggtcgc cgctgccgta gagaaatgac agccgcggcc
      241 ggatccagtc gaagtcatgt gacagctcca gggcgaagaa ctgcgcgttg atgcgctcgt
      301 tctgagcaat gaagctgccc tgccggtcgg tgccgagcgc ccagtagaag gaggtcgtga
      361 cggtccacga gccgaagtga ccgtcgccgt tgtagccgag gtagatcacg tcgtagtcgc
      421 gcgcgcgctc cagtcccagc gccgcgggcc gcacgatgaa accatcggta ttgaagtgaa
      481 cctcatcgcc ttcgcggtcg cggttgtaga ccaccgtcac ctgcgaggtg aagccctgga
      541 ccgggttgtc ctgcgagtac aggttgaaca ggaacacatc gtctttgcgc agcggtgcgc
      601 cgatatcgtt cagtccgctg ttggtgtcct tctcgatcaa ccggaagtag gcgatgttgt
      661 actgatagag gttgttgttg cgggtgccga acagccgcac accgagctga tcgtcctgaa
      721 acaggaagcc gcgaaagtcg gtcgaaaacg gttgaatgcc gacgcggatg ctgtcgaagt
      781 cgaacttctc ggatacctcg cgcaggcgca tctccgcgaa cgccgcctgc agcccggtga
      841 agtgatcact gcggttggtg ccctgacgcg ggtcgatgtt gacggcggtc agatcctcgg
      901 tcttcaggta gttgtaattg aagaccccgg tgaccttgaa ttgatagtcc ggtggcttga
      961 acacggtgtc gccgcgaaag agcgtgagat ccgcctcggc gctctgcgag aacacctgct
     1021 ggcgcgtgcc gccgaacacg ccgttgacac cggcgttggc cgtggagacc ccgcccacgg
     1081 gggtcaccac attctggatc tggtacaccg tatcggagat cagccccaga tcgaagaacc
     1141 agttgccgcc gaacaccggc ttgtcggcct tgagggtgtt ctggttgtac gggtcgaagt
     1201 agttctttgg atacccgagt gcatcgacga tgcgccagcg atccggcacc gcgacactct
     1261 gcgggaagtc ctgcatcgcc ggccggggca ccggggagac cacgctcggg gtgacttccg
     1321 cggcgactgt ctcgccggca ttcgcgccgt tgctgccggg ggccggctgg ctggtcggcg
     1381 ctccgacgcc cggctgcacg gtccgggttc cggggggcgg gcagggtact tcgacgtagc
     1441 ccgggcggcg gcgcgtcacg atgcgaccgg cgtcatcgcg gtagcaggtt tgcggcgggc
     1501 tctggggctg ggctgcctgc gtcggggcac tctgctgcgc ctgtgaaggc aaggcatacg
     1561 acagcaggcc gccgagcagc accgcggcgg ttgccgcgct gctgatcccc gttgaggccg
     1621 cgatggccgc tgcagttgac cgtcctggac actggctcat tcgcgacgag cctccctatc
     1681 ttcggcgatt attaattttg ggtggtacgt atggcaacta atacattgca gctggacctt
     1741 atcgcgttcc gaatccggcg tgtggcattc gaggcacttc ggcagatccg gaatcatcac
     1801 gaccgtgctt gagtctgtcg tgaccgcact atggcatgca agacaggcct cgtcgccggc
     1861 cttgcctttc tgcacttcat ggctgcgatg gctgaagtgc gcatccggga agtaatcgaa
     1921 cgtcagtcgt atcggggtga cctgatagcg ctcgaggatg tcggcggcct tggtgtccag
     1981 cacgacatga cagctcacgc agccgcggcg ggtgaactgg ttgtcgatct gcgcgcgcgc
     2041 cttctgcatc gcgcagtcaa aggtggccgc ccggcagatc tggctggtgt cctcatagtc
     2101 cacgctggcc tgatccggca gacgccggcg ctggaagggc ggcgcgctgg gcgggtgcgg
     2161 gtccaccacc tggcgtgcgt agaagtcctc gatgagcatc atggcatccc ggggcttgcc
     2221 atgcggcagg gagcgcttgg gagccgaggg gtcgaacgtc agttcatggc aggtgatgca
     2281 ggagcgcagc atcgtgaccg gaatgaagtg ctcgccatcg gcgccgagcg tatggcagtc
     2341 ggcgcaggtc agcggtgcgc tgtcgcccgg tcggcggacc cggctcggct ccaggtgctg
     2401 ggcatgggag aacttcaggt tggactgctc ggccgcctgg gcgatcggcg ctcgtaccgt
     2461 ggaccatttc agttcggcca cggttcccgg cgcgctgaag gcaggcttca gcagatccac
     2521 cgtgaaggcc gggtgctcgc tgcgggagaa cccggtgacc gccgtgacct tgattgggcc
     2581 aaagctgcgt tccgaggagg cgtgacaatc ggtacacagc gcgttactgc ggatgatcac
     2641 attggcggaa ctcgggttgt gctcttcgtg acactgggcg cagggctgct gcgggccgag
     2701 cttggtctgt gccaggtgcg ccggaatcac gtgatcgccg acgctcttgt ggcactcgcg
     2761 gcaggcactg tcgcgcacat gcacgaacag ggtttcgtgg cagccgtcgc agcgctgccc
     2821 ggtcgccagc tggtgcgcgg gcgatagcgg tccggcggtc cagaaggtgt cgtccgggat
     2881 gccggccggc gtgccgccgc cgccgcgatg cagatagatg gaggcgagcg gcatcaggaa
     2941 tgcgccgagc agtgccagca gcagcaggcc ccatgccgca cggcgcttgg tcagccaggt
     3001 ctcggacaga tcggtgcgga aggcgcgctc gaaactgctg gcactgacgt tcggatcggg
     3061 ccggatctcc agtgccagct cgaaccccat cggcggctcc accagctgca gttgatggcc
     3121 cgcgatctcc agcttgtcgc ccagcttcag cgtcgaggtg ctgactggct taccgttgag
     3181 ctgtacccgg cgtcccgcgc ggcaggtcag ggtcacggtg ccgccgctac tgcggatgag
     3241 ggcatgctgc gcgccgacgg cctcgcccag cagctgaatg ctgcagtttg cggaactgcc
     3301 gatcgtcagc gcatcggccg acagctcggt gtcgtgatac tcaaccagac cgtccggccc
     3361 gtccttgagt tgccggagta gggcgtccat cgcttaccag tacatgaaag tggtgagaat
     3421 gtgcgcgatc agcgcggcca gcagcgcgaa ggtgagcggc acgtgcacgt acagccacag
     3481 ccgcagccat ccctgcaccc ggatgtcgcg ccggatgcgt cgcagcaccg cctgacggcg
     3541 gctcaggacc gcgaccagag cctgaagcgt tgccggctcg ccgcgcttgg aggtgcgtga
     3601 cacccggttg gcgacgaatt cgaccaccgc ctgctgatcc acgttgctcg ccagtctgga
     3661 gccggtgccc tcacgctcgg tgcgcagaaa ccaggagcgg tcgatactga agagctgcgc
     3721 atagacgcca ccgccgatgg tggtgcgctc gatgctgctc ttgaccgcaa cgctgaccgc
     3781 cggatcgcag gcctgtgcca gatcgcgacc cttcttgtcc agttcgaaca gctcggcgaa
     3841 cagttccgaa cgcggccggc cgccgctgtt ctgcgcgagc tggcgcgggt tggccagata
     3901 ggtatagacc ccgaagatgc cgctgccgat caccgcgcac atcagcacat aggccagcat
     3961 gtgcacgttc cagccaaacc gcagcgcgct gtggagcgtg gcgacgaaga tcagggccag
     4021 tcccagatag acatgtgccg aggtccagcc ctgcaccgat cccaggctcg aggcataact
     4081 gcgtttgcgg atccccagca tcgtcagcca caggatcagc agcgcgccga tcgtccccag
     4141 cgtgtagccc tgccaggtgc cgccactggg cgggtgattg ccgccctggg tgtaatagag
     4201 gaggaagcag ccgatggtca gtcccagtgc ccaccacaga tagcgcgacg cgcgaaagcg
     4261 caggatgccc tcgtggatca ccggcggcgc tcctccacca gatccgcgta ctggctgggg
     4321 ctgatgcgca tggccgctcc ggtcgggcag gccttcacgc aggcaggtcc tgtgggcacg
     4381 ctcacgcagg catcgcactt gaccgctttc ttgcctttgg ccttggccgc atcggtcggg
     4441 tggtagtgca cctcctcacc gacccccggt ccgcgtccta gcagcagcca ctgcagcagg
     4501 ctcggcttct tcggcgcctc atattccatg cggatgaccc cgtagggaca gttcacctgg
     4561 caattgccgc agccgatgca ggtgtcatcg atatagacct cgccggtcgg cgagcgcttg
     4621 atggcgttcg gcgggcaatc cttcatgcag tgcggctgct cacagtgccg gcaggagatc
     4681 ggaatgtgca cctgtgcgta gctggcgccg cgcttgcgat ccagccgcga gataccgtgg
     4741 tgcgtttccg cgcaggcctt ctcgcaattg tcgcagccga cgcacagcga ctcatcgatc
     4801 accagcacgt cggtggcctc ccccaggccc tcatccatca ggaagttcat cagcgaaccg
     4861 gactccgggc gcacctccat gcgcgcgttc tcggtgacac gctcactggc ggcccgctgc
     4921 aacggatcga tcagcgcgtc gcggcgctgt gtgagctgca ggaattccgc ccggcgtacc
     4981 tcgatcattt ccagcgccag ggtcgcgcgc gcggtctcgc ggcgcaccgg gtcacccatc
     5041 agcgccatct caccgagcag ctgcccggcc gcgatctgcg ccaccagcag ctccctgccc
     5101 tcgcgcaggc gagtctgcgt cacggccccc gagcgcacga tgaacaggct gtcggccgta
     5161 ctgccttcct tgtagatgat ttcgcccgcc ttgaagcggc gcacgctcac cttggtgccg
     5221 atctcgcgca ggtcgcgcag cggggcgcgc ggggcaaact gactcttgag ctcgcgcacc
     5281 acgaagatcc agtcgatgcc gttgcgcact tcggcgttgg aattcatcag tttcagcatc
     5341 gtgcggcggg gcgtctccac cagcacacag ccggccccg
//
LOCUS       DATMRM010000005         8069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4339997, whole genome shotgun sequence.
ACCESSION   DATMRM010000005 DATMRM010000000
VERSION     DATMRM010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4339997"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="VNZ06_00140"
     CDS             complement(<1..1111)
                     /locus_tag="VNZ06_00140"
                     /inference="COORDINATES: protein motif:HMM:NF017019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HWW19186.1"
                     /translation="MFRTYRQRWSLFFITAAWLAAQAAIAADAPAYTKPKVRAITSFV
                     RVDPATLDQRVSEALAVLRMAKAEFEKQGYETETLRIVTQPLAELVKGQSESQALAFL
                     KRIDDLSVKESFLPSVGPAMMRDSDDARTIRLAEKALSTLPNLQINTVIADEDGIHWK
                     VIQESAALVHYVSEHSVHSQGDFNFTATAMLKAYGPFYPGTWHDGPGKQLSIGLEGAN
                     VVQEVFARTHGDFPGSVAELTRQLTVHAKVAETIGERVAAAKGWSFMGVDPTPAPLGD
                     VSIGAAIETYTGAKFGSSGTLTAALAITSAVKAVAVKQIGYSGLMVPVMEDKRLAQRW
                     AEGSYHADDLLAYSAVCGTGLDTLPLPGDVSVEQLS"
     gene            complement(1113..1673)
                     /locus_tag="VNZ06_00145"
     CDS             complement(1113..1673)
                     /locus_tag="VNZ06_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWW19187.1"
                     /translation="MHQPLPPPRRIVTGHNADGRSCIIADGPSPAILTMPQRPGYRNA
                     NIWRTSASPAPVNAPDGIVEHRGVLPPRTGTVIRVIDIPPEDPDPHERERQSRAVFAS
                     MFPDAALNAMHPRHPGMHVTATVDYAILLQGELVAILEEGETTMRAGDILIQRGTLHA
                     WANRSAAIARIAFVLIDGMAVAPPGG"
     gene            1822..2211
                     /locus_tag="VNZ06_00150"
     CDS             1822..2211
                     /locus_tag="VNZ06_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWW19188.1"
                     /translation="MKRIWTIIGVRDVPASFKWYQSLFGQPDTAPGHDHWGQLCDPDG
                     TVLLCLHQWGAHEHPSLMSPDRAQPGNGLLLFFRVDDFDRAQERARALVSRLDEEPHL
                     NPSTETLEFSLRDPDGYYVTISALCAP"
     gene            complement(2215..2463)
                     /locus_tag="VNZ06_00155"
     CDS             complement(2215..2463)
                     /locus_tag="VNZ06_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19189.1"
                     /translation="MENPEFDLTQPRRFRNRLDLGYSAVSEREAQHQAQLSVQCHHHS
                     RITVQQNWHSGLGAPFEGRRYVSTRLIAGSLMPWDRAA"
     gene            complement(2502..3077)
                     /locus_tag="VNZ06_00160"
     CDS             complement(2502..3077)
                     /locus_tag="VNZ06_00160"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWW19190.1"
                     /translation="MWPIYSGFWSRFAAFVIDTLLITIGGFFFSGLVLTIVTRLLHKA
                     TTESGENALIFLTIPVAAWLYFAAMESSPGQSTVGKLAMKIKVTDLSGEKIGFGCATG
                     RFFAKFLSALSLGVGYVMAVFTSRRQSLHDKVAGTLVVQRSLLPPEIAKAEPAPPIST
                     AARTLIVIAGLLFNPTTIGLVAAIAIGFHRH"
     gene            complement(3169..3242)
                     /locus_tag="VNZ06_00165"
     tRNA            complement(3169..3242)
                     /locus_tag="VNZ06_00165"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3208..3210),aa:Cys,seq:gca)
     gene            complement(3307..3382)
                     /locus_tag="VNZ06_00170"
     tRNA            complement(3307..3382)
                     /locus_tag="VNZ06_00170"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3347..3349),aa:Gly,seq:gcc)
     gene            3519..4106
                     /locus_tag="VNZ06_00175"
     CDS             3519..4106
                     /locus_tag="VNZ06_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWW19191.1"
                     /translation="MSGKKSLHSIVLAAGASRRFGSPKQLVRFDGQLLVQRVIAAATE
                     LTGPAVTVVLGAHAAEIAGNLPPGSASILINRGWQEGIASSIRLAVSRLPGACDGALI
                     MLADQPLVAAAGLTRLAAAWRRQTRSIIASRYGVVTGVPAIFPRWSFNDLAALRGDQG
                     ARLLLSRYHDHVVRLAHPEAAIDIDYPEDLLELGG"
     gene            complement(4120..5136)
                     /locus_tag="VNZ06_00180"
     CDS             complement(4120..5136)
                     /locus_tag="VNZ06_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWW19192.1"
                     /translation="MTAVHHGHDDHGHDHDHAHDHAHDHDHDHGHDEHDPDHHHGKGA
                     SESALLWALWITAGFMLAEAIGGWIAGSLALVADAAHMLADAGALAMSYGAVRAANRP
                     ATDVMSYGHHRWQVLAAFVNGLALLVLSVWIIIEALHRLLSPTAVQASVMMGVAGVGV
                     LANLLSYWVLSRGDRSLNVRSALLHVASDILGNVGAVLAGLVILLAHWTPIDPILSIV
                     MAALIVRGGYRVTRESAHILLEGSPAQFDVDAVEREIKAAVPSVTAVHHVHAWCLTDE
                     RPMLTLHVVIAEGADHTQSLKGVLHALRENLNIEHATVQIETTACGDDCSHLPRDVQQ
                     VRVG"
     gene            5212..5661
                     /gene="cadR"
                     /locus_tag="VNZ06_00185"
     CDS             5212..5661
                     /gene="cadR"
                     /locus_tag="VNZ06_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Pb(II)-responsive transcriptional
                     regulator"
                     /protein_id="HWW19193.1"
                     /translation="MRIGALAERAQCQAETIRYYEREGLLPIPPRSQGNYRLYGPAHI
                     ERLSFIRHCRSLDMTLDEIRTLLNFCDAPMSNCAQVSSVLDQHIHHVADRISQLRHLE
                     RQLKELRNQCASPGHAAGCGILKEMSRAAKRGQRALAPGHVHGSHTR"
     gene            5716..6411
                     /locus_tag="VNZ06_00190"
     CDS             5716..6411
                     /locus_tag="VNZ06_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWW19194.1"
                     /translation="MALAANTILERNRLFRGLSPQTIQQIASLSLRRPYSMGTIIFSQ
                     GDPGDALYGVVTGKVRISASAPDGKEMFLNIMEPGDTFGEIALLDGNPRTATASAMAP
                     TELLVIMRDPFLTLLQREQSLSTHLLRLLCQRIRWTSGLAEDSALLALPARLARRVLS
                     LAKLHGHQVLNGVHLAISQEEIARFLGISRQVVNQYLQTWKAQGWVELGRAKLTILDE
                     QALQRTAESSDQR"
     gene            6507..7889
                     /locus_tag="VNZ06_00195"
     CDS             6507..7889
                     /locus_tag="VNZ06_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HWW19195.1"
                     /translation="MQTECNAEQLQFSCVERRRVVAAFDGGTVSSDAGALLLGRADEA
                     IGLIDRLAGCFIDARRPDLIEHTVRTMIGQRVFGIALGYEDLNDHEQLRHDPVFGALL
                     GKLEARRSDCAALAGKSTLNRLELYAREGVDPYHKIRPRGEAIERLFVELFLEAHRQR
                     PDEIVLDLDATDDPLHGHQEGRFFHGYYDGYCYLPLYIFCGEHLLSSKLRPANIDGAA
                     GAREEIERIVAQIRERWPQVRIILRADSGFCREPLMSWCEQNAVDYVFGLARNTRLVR
                     SIGAELHEAAAESAQTYRAARRFKELSYRTRRSWSRARRVVAKAEQTGDKANPRFIVT
                     SLSMHDWPMRELYEELYCARGEMENRIKEAQLNLFADRLSTESFRANQLRLWLSSAAY
                     VLMHALRRIGLAGTSLARACANTIRLRVLKIGAVVTLSVRRVKLAMSGACPNQREFIA
                     AFHALSAAAR"
BASE COUNT         1528 a         2533 c         2496 g         1512 t
ORIGIN      
        1 ggcttagctg ttcgacactc acatcgccgg gcaacggcag cgtgtcgaga cccgtcccgc
       61 ataccgcgga ataggcgagc agatcgtcgg cgtgatagct gccctcggcc cagcgctgcg
      121 cgagccgctt atcctccatc acaggtacca tcaggcccga atagccgatc tgcttcaccg
      181 cgaccgcctt caccgcgctg gtaatcgcca gtgcggccgt cagcgtgccg ctcgaaccaa
      241 acttcgcgcc ggtataggtc tcaatcgccg cgccgatcga tacgtcaccg agcggcgcgg
      301 gcgtcgggtc cactcccatg aagctccagc ccttggcggc ggccacgcgc tcgccgatcg
      361 tctcggccac cttggcatgc acggtgagtt gtcgggtcag ttccgcaacc gagccgggaa
      421 aatcgccgtg agtgcgcgcg aacacttcct gcacgacatt cgcgccctca aggccgatcg
      481 acagttgctt gcccggaccg tcgtgccacg tgcccgggta gaagggtcca tacgccttca
      541 gcattgccgt ggcggtgaaa ttgaagtcgc cctggctgtg aacgctgtgc tcgctgacgt
      601 aatgaaccag cgcggcgctc tcctggatga ccttccagtg aatgccatcc tcatcggcga
      661 tcaccgtgtt gatctgcaga ttcggcagcg tggaaagcgc cttctcggcc agccgtatgg
      721 tgcgcgcatc atcgctgtca cgcatcatcg ctggccccac actcggcagg aagctctcct
      781 tgacggacag atcatcgatg cgtttgagaa aggccagcgc ctgcgattcc gactgaccct
      841 tgaccagttc ggccaacggc tgcgtcacga tgcgcagcgt ctcggtctca tagccttgct
      901 tttcgaactc ggccttggcc atgcgcagca ccgcaagcgc ctcgctcacc cgttgatcga
      961 gcgtggcggg atcgacccgc acaaacgatg tgatcgccct gaccttgggc ttggtgtagg
     1021 ccggcgcatc ggcagcgatc gccgcctgag cggcgagcca tgccgctgtt ataaagaaga
     1081 gtgaccaacg ttggcgatag gttcgaaaca tctcagcccc ctggtggcgc tacggccatc
     1141 ccgtcgatca gtacgaacgc gatacgggca atggccgctg agcggttggc ccacgcgtgc
     1201 agggtgccgc gctgaatgag aatgtccccc gcacgcatcg tggtctcgcc ttcctcgagg
     1261 atcgcgacca gctcgccctg cagcaggatc gcataatcca ccgtggctgt gacatgcatg
     1321 ccaggatgac gagggtgcat ggcgttgagc gccgcgtccg ggaacatcga ggcgaacact
     1381 gcgcgcgact gtcgctcgcg ctcgtgcggg tccggatcct ccggcggaat atcgatcacg
     1441 cgaatcaccg tacccgtccg gggtggcagt acgccgcggt gctcgacgat gccgtccggc
     1501 gcattcaccg gggcgggact tgcgctggtg cgccagatat tggcgttccg atatcccggc
     1561 cgctgcggca tcgtcaggat cgccggcgac ggcccatcgg cgatgatgca ggatcgcccg
     1621 tcagcgttgt gaccggtcac gatgcgtcgt ggcggaggca gtggctgatg catagactgt
     1681 tcgctcccgc gcgatccgga aattgataaa ggttgctgtg cacaagggcc gccgcagttt
     1741 gcgcgcaatt gagcctgtca atccggatct tgactatgat tcgcggatgc attggcgcat
     1801 cgcccggagg ctgtccacac catgaaacgc atatggacga tcatcggcgt cagagacgtt
     1861 ccggccagct tcaagtggta ccagtcgctc tttggtcaac ctgatacggc tccgggccat
     1921 gaccactggg gtcagctgtg cgatccggat ggaaccgtcc tgctgtgcct gcatcagtgg
     1981 ggcgcgcatg agcatccctc gttgatgagt ccggaccggg cgcaacctgg aaacgggctg
     2041 ctcctgtttt tccgtgtcga tgatttcgac cgggcacagg agcgagcccg ggcgctggtg
     2101 tcgcgactcg acgaggagcc tcacctcaat ccgagcactg agacgctcga gttctctttg
     2161 cgcgacccgg atggatacta tgtcaccatc agcgcgctct gtgcaccgta gccttcaggc
     2221 cgcccgatcc cacggcatga ggctgccggc gatcagccgc gtgctgacgt agcgccgacc
     2281 ttcaaagggc gcgcccaggc cgctgtgcca attctgctgg acggtaatcc gggaatggtg
     2341 gtggcactgc acgctaagct gcgcctggtg ctgcgcctct cgttccgaga cggcagaata
     2401 tccgagatcg agacgattgc ggaaccggcg cggctgagtc aaatcgaact cggggttctc
     2461 caagccatgc tcattgacat cgcgccggca ctgacccgta ttcagtggcg atgaaacccg
     2521 atggcaatcg ctgcgaccag accgatcgtc gtgggattga acagcaggcc ggcaataaca
     2581 atcagggtcc tggcagcggt gctgatcggc ggtgctggct ccgctttcgc tatctccgga
     2641 ggcagcagcg atcgttgaac gaccagcgtt cctgctacct tgtcgtgcaa tgattgacgg
     2701 cggctggtga ataccgccat gacatagccg acgccgagcg acagtgccga caggaatttc
     2761 gcaaagaatc gtccggtcgc acacccgaat ccgatcttct caccactcag atcggtcacc
     2821 ttgatcttca tcgcgagctt gccgaccgtg ctctgtcccg gtgaggactc catcgctgcg
     2881 aagtacagcc aggcagcgac cgggatcgtc agaaaaatga gagcgttttc gcccgactca
     2941 gtggtggctt tgtgaagcag ccgagtaacg atggtaagga ccaagcctga aaaaaagaaa
     3001 ccgccaatgg tgatcagcag ggtgtcgatg acaaaagctg cgaatcgact ccagaagccg
     3061 gagtaaatcg gccacatctc agcgccctcc cttccgccgt atggtagctc ggaggcgagc
     3121 agtgagattc gcctatcgtt taccgatggg cgttatggaa gcttcgtctg gaggctaggg
     3181 tcggaatcga accggcgtcc acggctttgc aggccgctgc atgaccactc tgccacctag
     3241 ccaatcaaga tctgaaaaaa acccttccgg ccgcgggcgc caaaaagggc ttatgtcgcg
     3301 catcattgga gcgggaaacg aggctcgaac tcgcgacctc aaccttggca aggttgcgct
     3361 ctaccaactg agctattccc gcgccttggg aagaccggaa tcttaagtcc gccgacgggc
     3421 aagtcaagat attatcccaa aaccatggat ctacagtgtg gaacctgtgc cgccgaactc
     3481 actgaagcct cgcgctgcgt gatctgtcgc tgcatgtgat gagcggcaag aaatccctgc
     3541 actcgattgt tctggccgcg ggcgcatcgc gacgtttcgg ttctccgaaa cagctggtgc
     3601 gctttgacgg acagctattg gtgcagcgtg tgatcgctgc cgcgacggag ctcaccgggc
     3661 ccgcggtgac cgtggtattg ggggcgcatg ccgccgagat cgctggcaat ctgcctccgg
     3721 gcagcgcgag cattctcatc aatcgcggct ggcaggaagg catcgccagt tcgatccgcc
     3781 tggcggtgtc gcggctgccc ggcgcgtgcg acggcgcgct gatcatgctg gccgatcagc
     3841 cgttggtcgc tgcagcgggc ctgacccggc tggcagcggc gtggcgacgg caaacccgca
     3901 gcatcatcgc ctcgcgttat ggcgtggtca ccggcgtgcc tgctattttc ccgcgctgga
     3961 gtttcaacga tcttgccgcg ctgcgtggag atcagggtgc caggcttctg ctcagccgct
     4021 accacgatca tgtggtgcga ctcgcgcatc ccgaagcggc catcgatatc gactatcccg
     4081 aagatttgct cgaactgggt ggctgagccg ccggagcttt caaccgaccc gcacttgctg
     4141 cacatcgcgc ggcaggtgtg agcagtcatc cccgcaggcc gtggtttcga tctgcacggt
     4201 cgcatgttcg atgttgaggt tctcgcgcag tgcatgcagc acacccttca ggctctgcgt
     4261 atggtctgcg ccctcggcaa tcaccacatg cagcgtcagc atcggccgct cgtcggtgag
     4321 gcaccaggcg tgcacgtgat ggaccgcggt gacggacggc accgccgcct tgatctcgcg
     4381 ttccaccgca tccacgtcga actgcgccgg cgagccctcc agcaggatat gcgccgactc
     4441 gcgcgtcacc cgatagccgc cgcggacaat cagggccgcc atgacgattg acaggatcgg
     4501 atcgatcggc gtccagtgcg ccagcaatat caccaggccg gccagcacgg cacccacatt
     4561 gcccaggatg tcggaggcca cgtgtagcag cgcgctgcgc acgttgagac tgcggtcacc
     4621 gcgcgacagc acccaatagc tgagcaggtt ggcaaggacg cccacacccg ccacccccat
     4681 catcacggaa gcctgcaccg cggtcgggga tagcagacga tgcagcgcct cgatgatgat
     4741 ccagacggac agcaccagca gcgccaggcc gttgacgaac gcggccagaa cctgccaacg
     4801 atgatggccg tagctcatga cgtcggtggc cggccggttg gcggcacgca ccgccccgta
     4861 gctcatggcc agggctccgg catcggccag catgtgcgcc gcgtcggcta ccagcgccag
     4921 cgaaccggca atccagcccc caatggcctc ggcgagcatg aatccggccg tgatccacag
     4981 ggcccacagc agcgccgatt cactcgcgcc cttgccgtga tgatgatcgg ggtcgtgctc
     5041 gtcatgcccg tggtcatggt catggtcatg agcatggtca tgagcatgat cgtggtcatg
     5101 tccgtgatcg tcatgtccgt gatgaacggc cgtcatttgc gcattagaga ccctgtagct
     5161 actctagagt caagtctgga gcccgctctt aggatcaata tgagagatgg attgagaatc
     5221 ggagcattgg ccgaacgtgc ccagtgccag gcagaaacca ttcgctacta cgagcgggag
     5281 ggactactac ccattccacc acgctcgcaa ggcaactatc gcctgtacgg ccccgctcac
     5341 atcgagcgcc tgtcgttcat ccggcactgt cgctcactgg acatgacgct ggatgagatc
     5401 cgtaccctgc tgaatttctg cgatgcgccg atgagcaatt gcgcgcaggt cagctcagtg
     5461 ctggatcagc atattcatca tgtcgcggac cgcatttcgc agctgcgtca tctggaacgc
     5521 cagctcaagg agctgcgcaa ccagtgcgcc agtcccggac acgcggccgg ctgtggcatc
     5581 ctcaaggaga tgtcacgcgc cgcgaagcgg ggtcagcgcg cgctggcccc gggccacgtg
     5641 catggctcgc atactcggtg ataatcccgt tatattttag atatttataa cattatacta
     5701 atgtaggata tagccatggc tctggctgcc aataccatcc tcgagcgcaa ccggctgttt
     5761 cgcgggttgt cgccgcaaac gattcagcag atcgcttcgc tgtcactgcg ccgcccctat
     5821 tcgatgggca ccatcatttt ctcgcagggc gatccgggcg acgcactcta tggggtcgtg
     5881 accgggaaag tgcgcatcag cgccagcgcc ccggatggga aggaaatgtt cctcaacatc
     5941 atggaacccg gagacacgtt cggcgagatt gcactgctgg atggcaatcc acgcacggcg
     6001 acggcgagcg ccatggcgcc gacggagttg ctggtgatca tgcgcgatcc attcctcacg
     6061 ctcttgcagc gcgagcagag cctgtccacg catctgctgc gactgttgtg ccagcgcatt
     6121 cgctggacca gcgggttggc cgaggattcg gcgctgctgg cactgccggc gcggctggcg
     6181 cgccgtgtgc tgagtctggc gaaactgcac ggccatcagg tactcaacgg cgtgcacctc
     6241 gcgatctcgc aggaagagat tgcgcgtttc ctgggaatct ctcgccaggt ggtcaatcag
     6301 tacctgcaga cctggaaggc tcaaggctgg gttgagcttg gccgcgccaa gctcacgatc
     6361 ctggatgagc aggcgctgca gcgaaccgcc gaaagcagcg atcagcgcta gccgggatat
     6421 ctcatacgcg gagggtgcat cgggcctgtg agtaggtaaa aatccgttta ccacaacgag
     6481 ttaaacctac ccacaggaga gtcccgatgc agacagagtg taacgccgaa cagctgcagt
     6541 tttcatgtgt tgagcgccgc cgggtggtgg ctgcgttcga cggcgggacg gtcagttccg
     6601 atgccggggc actgctgctg gggcgcgcgg atgaggcaat tggtctgatc gatcggctag
     6661 ccggctgctt catcgatgcg cgacggcccg atctgatcga acacacggtc aggacgatga
     6721 tcgggcagcg cgtgttcggg atcgcgctgg gctatgagga cctgaacgat cacgaacaac
     6781 tgcgccacga tccggtgttt ggcgcactgc taggcaagct tgaggcgcgt cgatccgact
     6841 gcgcggcgct ggccggcaag agcacgctca accgtctgga attgtacgca cgtgaagggg
     6901 tggatcccta tcacaagatc cgcccccggg gcgaggcgat cgagcgcttg ttcgtggagc
     6961 tgttcctgga ggctcaccgc cagcggcccg atgagattgt gctggacctg gacgccacgg
     7021 atgatccgct gcacgggcat caagagggac gcttctttca cggctactac gatggctact
     7081 gctatctgcc gctgtacatc ttctgcggcg agcacctact gagttccaaa ctgcggcccg
     7141 cgaacatcga cggggcagca ggcgcacgcg aggaaattga gcgcatcgtg gcacagatcc
     7201 gcgagcgctg gccgcaggta aggatcatct tgcgtgccga ctcgggcttc tgtcgcgagc
     7261 cgctgatgag ctggtgcgaa caaaacgctg tggattacgt gttcgggctg gcccgcaaca
     7321 ctcgcctggt gcgctcgatc ggcgctgagc tgcatgaggc ggcggctgag tcggcgcaaa
     7381 cctaccgagc ggcgcggcgc ttcaaggagc tcagctaccg cacgcgccgc agctggagcc
     7441 gtgcacgacg cgtggtggcc aaggccgagc agaccgggga caaggccaac ccgcgcttca
     7501 tcgtcacttc gctgtcgatg catgactggc cgatgcgcga gctgtacgaa gagctctact
     7561 gcgcgcgcgg tgagatggaa aatcgcatca aggaagcgca gctcaacctc tttgccgatc
     7621 gattgtccac cgagagcttt cgcgccaatc agttgcgtct atggctgtcc tcggcagcct
     7681 atgtgctcat gcatgcgctg cgccgcatcg gattggctgg caccagcctg gcgcgcgctt
     7741 gcgccaacac gatccgactg cgtgtgctca aaattggcgc ggtagtgacg ctgagcgtac
     7801 gacgggtgaa actggcgatg agtggggcct gtccgaatca gcgcgagttc atcgccgcct
     7861 tccatgccct gagcgctgcg gcacgctaag ccgcacccac gaaccagact cccacgctct
     7921 caatcgtcca accgccctct cgaagagcgg cgcatcggcg tctctgaaaa ccgtccaaat
     7981 ccgccctcgc gcactcccgt gcccactccg ccgtctccgc ccggatcagg ctgcaatatc
     8041 agcgaggggt atgagatatc ccggctagc
//
LOCUS       DATMRM010000006         4852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1552657, whole genome shotgun sequence.
ACCESSION   DATMRM010000006 DATMRM010000000
VERSION     DATMRM010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552657"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /gene="flhF"
                     /locus_tag="VNZ06_00200"
     CDS             complement(<1..1206)
                     /gene="flhF"
                     /locus_tag="VNZ06_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HWW19196.1"
                     /translation="MKMKRYVAADMRSALRAIREEQGPDAVILSSRPTSNGVEVCAAV
                     DLELAAGQGTLAEEAALKQLERVALSELQREAQQVAANALRPANQAADEHEELSRAAR
                     ATDAAELSSSVGEELKSLRGLLEQQLAALAWNDFTRREPLKARALTDLANLGLERALA
                     MEIVEEMPASVRADQAQRLPYALLSRRLKVCPPPHERSGAIALIGPTGSGKTTTLSKL
                     AARYVLEQDAANLIVISADDERLGAHEQLRSLGRLLGVRVESVSSLDEVAARIAALPD
                     RMILIDTPGIVSRDSEAVARYRRWRDKCRALQIMLTLPASAQGGVVDEAVIGFNGVAT
                     CCVLTRVDEAVSLGGVLSSLARCKLPVAYCCEGPRIPDDIRPVRAHQLIARAMDLARQ
                     AQVCADDDLL"
     gene            complement(1231..3282)
                     /gene="flhA"
                     /locus_tag="VNZ06_00205"
     CDS             complement(1231..3282)
                     /gene="flhA"
                     /locus_tag="VNZ06_00205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HWW19197.1"
                     /translation="MLQRGAGAPLLLLAVLAMVVLPLPPLALDVLFTFNIALSVLVVM
                     AVVNVARPLDFGIFPTVLLLATLLRLALNVASTRVVLLHGNEGTAAAGHVIEAFGQFV
                     VGGNYAVGIVVFAILTIINFVVVTKGAGRISEVSARFTLDAMPGKQMAIDADLNAGLI
                     NQDEARVRRAEVRSEADFYGAMDGASKFVRGDATAGILILVINIVGGLAIGTLSHGMG
                     FTDAARIYTLLTIGDGLVAQIPALLLSTGVAIIVTRMSRAQDLGGELSKQLFRQPKSL
                     AVAASLLGVMGLIPGMPNLAFLLFGAACGTGAWLLNRRAKRAAQAPAAAAPPPPVVNP
                     EHKELSWEDVQPVDQIGLEVGFRLVRLVDKAQGGELLARIRGVRRKLSQELGFLVQSV
                     HIRDNLELQPNAYRISLAGVAIADGIVHPDRELAINPGRVFGKPPGIETRDPAFGMEA
                     IWIEPSKREHAQTLGYTVVDASTVVATHLSHVIQTHAHELLGHEEVQQLLNGLAKTAP
                     RLVEDLVPKALPLSVVVRVLQGLLAERVPIRNLRAIVETLAEHAPRSQDAGVLSAQVR
                     IALGRQIVQDIVGGAAELPVITLEPDVEQLLQGSLQGQGAGGSAAIEPGLAERLQARL
                     AESARRLEAAGDPSVLMVAPQLRNTLARFTRATVPNLHVLAWNEIPDNRRVRLVAAVG
                     R"
     gene            complement(3443..4567)
                     /gene="flhB"
                     /locus_tag="VNZ06_00210"
     CDS             complement(3443..4567)
                     /gene="flhB"
                     /locus_tag="VNZ06_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HWW19198.1"
                     /translation="MAEDVNDSERTESPTQKRLDDARAKGQVPRSRDLSAAAVVLAGG
                     LGLRTLGSQFGGNMVSLMRESLTFTRDAALDNGWMLHQFERSALQGALAMAPLLCLLL
                     AAAVLAPLAIGGWTFSGEALVPDFGRLNPAAGLGRVFSVRGLIELSKALARFIVVAIV
                     AVALLWKQFHSFNQLELEPTGAAVAHAVLLAGTALIALGGALAAIAMVDVPLALWQFN
                     KQMRMTRQEIREEHKETEGSPEIKSRVRRTQQAMARKRMMQQVPKASVVITNPTHFAV
                     ALQYDESRMRAPIVVAKGRDLVAARIREIAAEHNVALVEAPPLARALHASCELGDEIP
                     ARLYAAVAKILVYVYQLRTARGRGEPSPVPPSIELPEQGD"
     gene            complement(4571..>4852)
                     /locus_tag="VNZ06_00215"
     CDS             complement(4571..>4852)
                     /locus_tag="VNZ06_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HWW19199.1"
                     /translation="MRSMAEWGDQLFVGALKVALPAMTALLVVNLAFGAVSRAAPALN
                     MFAIGLPVTLIFGLVVLFFGLPSMQSGFVELMKSSFQFMQALTGAGLGA"
BASE COUNT          894 a         1681 c         1474 g          803 t
ORIGIN      
        1 cagcaggtca tcgtccgcac acacctgtgc ctgccgtgcc agatccatcg cccgtgcgat
       61 cagctgatgc gcgcgtaccg ggcgaatgtc gtcaggaata cgcggccctt cgcagcagta
      121 ggccactggc agcttgcagc gcgccagcga cgacagcacc cctccgaggc tcaccgcctc
      181 atcgacgcgc gtcagcacac agcaggtcgc gacgccattg aaaccgatta ccgcctcatc
      241 gaccacgccg ccctgcgcgc tggccggcag tgtcagcatg atctgtaagg cacggcattt
      301 gtcgcgccag cgtcggtagc gcgcaacggc ctcgctgtcg cgactcacga tcccgggggt
      361 gtcgatcaga atcatccgat ccggcaacgc tgcgatgcgc gccgcgactt catccagact
      421 gctcactgac tcgacgcgca caccgagcag gcgcccgaga ctgcgcaact gctcgtgcgc
      481 acccagtcgc tcgtcgtcag ccgagatcac gatcagattg gcggcgtcct gttcgagcac
      541 gtagcgagcc gcaagtttcg acagtgtcgt ggtcttgccg gagccggtcg gtccgatcag
      601 cgcgatcgcg ccactgcgct cgtgcggcgg cggacacacc ttcagccgcc tcgacagcag
      661 cgcgtagggc agacgttgag cctggtcggc gcgcacggat gctggcattt cctcgacaat
      721 ctccatcgcc aacgctcgtt ccagccccag attggccaga tcggtcaagg cacgcgcctt
      781 caagggctcg cgccgcgtaa aatcattcca cgccagtgcc gcgagctgct gttccagcag
      841 cccgcgcagg ctcttgagtt cttcacccac cgacgaactg agctccgcag catccgtagc
      901 gcgtgccgcg cggctcaact cctcgtgctc gtccgcagcc tgatttgccg ggcgcagcgc
      961 attggcggcg acctgctgag cctcgcgctg cagttcgctg agtgcgacgc gttcgagttg
     1021 cttgagcgcg gcctcctcgg ccagcgttcc ctggccggcg gccagctcga ggtccaccgc
     1081 cgcgcagact tcgaccccgt tgctggtagg ccggctgctc aggatgaccg catcgggccc
     1141 ctgctcttcg cgaatggcgc ggagtgcact gcgcatatcc gccgccacat atcgcttcat
     1201 tttcatgcgc gctgtctctc catctcgacg ttaacgaccg accgccgcga ccaggcgtac
     1261 ccgccggttg tcgggaattt cattccatgc cagtacatgc agattcggca cggtggcacg
     1321 cgtgaagcgc gccagcgtgt tacgcaactg cggcgcgacc atcagcaccg acggatcgcc
     1381 tgccgcttcc aggcgtcgtg ccgactcggc cagtcgcgcc tgcaggcgtt ccgccagccc
     1441 gggttcgatc gccgcactgc cgccagcgcc ctgaccctgc agcgagccct gcagcagttg
     1501 ttcgacatcc ggttcgagcg tgatcacggg cagctccgct gctccgccca cgatgtcctg
     1561 aacaatctga cggcccagcg cgatgcgcac ctgtgcgctc agaacgccgg cgtcctgcga
     1621 gcgcggagcg tgctcagcga gtgtttcaac gatcgctcgc agattgcgaa tcggtacccg
     1681 ctcggccagc agaccctgca gcacccgcac cacgacgctc agcggcaacg ccttcggcac
     1741 cagatcctcc accaggcgcg gcgccgtctt cgccagccca ttgagcagct gctgcacttc
     1801 ctcatgaccg agcagctcgt gggcgtgagt ctgaatgaca tgactgagat gcgtcgcgac
     1861 gaccgtgctg gcgtcaacga cggtgtagcc gagggtctgc gcgtgttcgc gcttggaagg
     1921 ctcgatccag atggcctcca tgccgaacgc tggatcgcgc gtctcgattc ccggcggctt
     1981 accgaacacc ctgccaggat tgatcgccag ctcgcggtcc ggatgcacaa tgccatcggc
     2041 aatggcgacg cccgccagac tgatgcgata agcgttcggc tgcagttcca ggttgtcgcg
     2101 aatgtgcacc gactgcacca ggaacccgag ttcctgcgac agcttgcggc gcacgccacg
     2161 gatgcgtgcg agcagttcgc cgccctgcgc tttgtccacc aggcgcacca gacggaagcc
     2221 gacctcaagg ccaatctgat cgaccggctg cacgtcctcc cacgacaact ccttgtgctc
     2281 cggattgacc accggcggcg gcggtgcggc ggcggctgga gcctgcgcgg cccgcttcgc
     2341 ccggcgattc aggagccagg ctccagtgcc gcaggcggcg ccgaataaca gaaatgcgag
     2401 attcggcata ccagggatca gacccatcac gccgagcaga ctggctgcga ccgccagact
     2461 cttgggctgg cggaacaact gcttgctcag ctcgccgccc aggtcctgcg cacgagacat
     2521 gcgcgtcacg atgatggcga caccggtgga cagcagcagc gccggaatct gcgccaccag
     2581 gccgtcgccg atcgtcagca gcgtgtagat acgcgccgcg tcggtgaacc ccatgccgtg
     2641 actgagcgtg ccgatcgcca ggccgccgac gatgttgatc accagaatga ggatgccggc
     2701 ggtggcgtcg ccgcgcacga acttgctggc accgtccatc gcaccgtaga agtccgcttc
     2761 cgagcgcacc tcggcgcgac gcacccgcgc ctcatcctgg ttgatcagtc cggcattcag
     2821 gtcggcatcg atcgccatct gcttgccggg catcgcatcc aacgtgaacc gcgcgctgac
     2881 ttccgagatg cgcccggcac ctttggtgac cacgacgaaa ttgatgatcg tgagaatggc
     2941 gaacacgacg atgccgacgg cgtaattgcc gccgacgacg aactgtccga aggcctcgat
     3001 cacgtgaccg gcggcagcgg tgccttcgtt gccgtgcaga agcaccacgc gggtcgaggc
     3061 gacgttgagt gccaggcgca gcaacgtcgc cagcaacaac actgtcggga agatgccgaa
     3121 gtccagcggg cgcgcaacat tgaccacggc catgaccacc agcaccgaca gcgcgatatt
     3181 gaacgtgaag agcacatcca gcgccaacgg tggcagcggc aagacgacca tcgccagtac
     3241 ggccagcagc aatagcgggg cgccagcacc ccgctgtagc attctgacta gtccaggcga
     3301 gcctgccgcg ttggcagcag aagcgttcat cgcgagttca gccatagctt gaagaggagc
     3361 aaatcttgtg ccgctcggca gcgacaattt aacgacgcat caggggctta agtaacgcga
     3421 cgtgcgtcac acaattggga cactaatcgc cctgttcggg cagctcgatg ctcggtggaa
     3481 cagggctcgg ctcgcctcga ccgcgcgccg tacgtagctg gtacacgtag accaaaattt
     3541 tggcgaccgc ggcgtacaga cgtgccggaa tttcgtcgcc aagctcgcag ctcgcgtgca
     3601 gtgcgcgcgc cagcggcggg gcctcgacca gcgctacgtt gtgctcggcc gcgatttcgc
     3661 ggatgcgagc ggcgaccagg tcgcggccct tggccaccac gatcggcgcg cgcatgcgcg
     3721 attcgtcata ttgcagcgct accgcaaagt gcgtcgggtt ggtgatcacc acgcttgctt
     3781 tgggcacctg ctgcatcatg cgtttgcgcg ccatggcctg ctgcgtgcgc cgaacgcggc
     3841 tcttgatctc aggcgagcct tccgtctctt tgtgctcttc gcggatctcc tgccgcgtca
     3901 tgcgcatctg cttgttgaat tgccagaggg cgagcggcac atcgaccatc gcgatcgcag
     3961 cgagtgctcc gccgagcgca atcaatgcag taccggcaag caacaccgcg tgagccacgg
     4021 cagcgccggt cggctcaagc tcgagttgat tgaaactgtg gaactgcttc cacaacagcg
     4081 ccaccgcgac gatcgccacc acgatgaagc gcgccagcgc cttgctaagt tcgatcagcc
     4141 cgcgaaccga gaacacgcga cccagtccgg cagcgggatt gaggcgcccg aaatcaggca
     4201 ccagggcctc gccactgaag gtccagccgc cgatcgcgag cggcgccagt accgcggcgg
     4261 ccagcagcag gcacagcagc ggcgccatcg ccagtgcgcc ctgcagcgca ctgcgctcga
     4321 actgatgcaa catccagcca ttgtccagcg cagcatcacg cgtgaaggta aggctctcgc
     4381 gcatgagcga gaccatgttg ccgccgaact gcgagccgag cgtgcgcagc ccgaggccgc
     4441 cggccagcac caccgccgct gcactcagat cgcgcgagcg tggcacctgc cctttggcgc
     4501 gagcgtcgtc taaccgcttt tgcgtcggac tttctgtccg ttcactgtca ttgacgtcct
     4561 ctgccatgac ctacgctccc aatcccgcgc cggtcagcgc ctgcatgaac tgaaacgagg
     4621 acttcatgag ttcgacgaag cctgactgca tgctgggtaa gccaaagaac agcacgacca
     4681 atccaaagat caaggtaacc ggtagaccga tcgcgaacat attgagcgca ggcgcggcgc
     4741 ggctcaccgc gccgaaggcc agattcacca ccagcagcgc ggtcatcgcg ggcagtgcaa
     4801 ccttcagggc acccacgaac agttgatcgc cccactcggc catcgagcgc at
//
LOCUS       DATMRM010000007         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1552782, whole genome shotgun sequence.
ACCESSION   DATMRM010000007 DATMRM010000000
VERSION     DATMRM010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552782"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VNZ06_00220"
     CDS             <1..337
                     /locus_tag="VNZ06_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19200.1"
                     /translation="AAPRYTIAGGVLYKTAHWKFSLIDKTVGPQFETADNSRHYELGS
                     YSNVDANAGVTIGPVELSATVSNLLNSRKVLSITQNDTTYQTNQLQSLDQYFFQPTRS
                     VMVTLKARF"
     gene            395..1378
                     /locus_tag="VNZ06_00225"
     CDS             395..1378
                     /locus_tag="VNZ06_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWW19201.1"
                     /translation="MSAALAGLLLDGCSSPRPTSTDASPATSTGLNVVSDAELHRPTR
                     FIVYGDMRFTARSETQASRPAPREALVARIAVERPDALFLTGDVPWHGGNEDDYAVYR
                     QETTQWRSEQLRVYPVLGNHEFQRCAEEDCLANWWKAFPQFRARRWYAVALGSQLRFL
                     ALDSNGSLLPGSEQAQWLQDQIQALPASVRFVFILLHHPLMTDGHEGVRANEAALAAQ
                     LAAVAPHAPARFVVCTAHVHNYERFERDGVVFLVSGGGGAKPTPITRSSADRYRKDDF
                     PNFHYIRFELTSRGLRGEMVRLSDAESTNLVTWAVNDRFEIDQFRTTRTID"
     gene            complement(1569..>2475)
                     /locus_tag="VNZ06_00230"
     CDS             complement(1569..>2475)
                     /locus_tag="VNZ06_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWW19202.1"
                     /translation="SCIQTAVTRKCSMTHSPPVAIVTGASRGAGKGIAIALGSHGCVV
                     YVTGRSQREGDASMPGTIYATAEAVTKAGGKGIAVRCDHADDAQVKAMIGQVTREQGR
                     IDILVNNACAIHDRLPGPGNFWEKPLEIGNMINVGVRSGFAASWLVAPTMVKQGSGLI
                     VFTSAPGSVYYCLGPAYGAHKAGVDKMAFDMAVDFKDAGVNVAAVSIWMGSLMSERLV
                     QLMQAAPNKFRHLEGTLESTEFTGHVIWALFKDTKLVEVSGKTLIGAELGRHYGITDL
                     GGKYPPSVREMHRCAPHEYSPFKVK"
BASE COUNT          493 a          762 c          705 g          515 t
ORIGIN      
        1 tgcggcaccc cgctacacga tcgctggcgg cgtcctgtac aagacggcac attggaaatt
       61 ctcgctaatc gacaagacgg tgggccctca gtttgaaacc gccgacaatt cacggcacta
      121 cgagctgggc tcctacagca acgtcgatgc caatgccggc gtcaccatcg gtccggtgga
      181 actgtctgcg accgtcagca acctgctcaa tagtcgcaag gtattgtcaa tcacccaaaa
      241 cgatacgacc tatcagacca accagttgca gagcctggac cagtatttct tccaacccac
      301 gcgcagtgtc atggtgacac tgaaagcgag gttctgatcg ccgcaggggg cagactgcat
      361 gcgaatcgtg cgccgccgta taccggcaca gctcatgagc gcagctctgg ccggcttgtt
      421 gctggacggc tgttcttcgc ccagaccgac gtccacagac gcctcacctg cgacgtcgac
      481 cggcctcaat gtggtttctg atgctgaact tcatcggcca acgcgcttta ttgtgtacgg
      541 cgacatgcgc ttcacggcta ggagcgagac gcaagccagc cggcctgcgc cgcgcgaagc
      601 gctggtggcg cggattgccg tcgagcgccc ggatgcgttg ttcttgaccg gcgatgtccc
      661 ctggcacggc ggcaatgagg acgactatgc cgtctaccga caggagacca cgcagtggcg
      721 atcggaacag ctgcgcgtat atccggtgct cggaaatcat gagtttcagc gctgcgcgga
      781 agaggactgt ctcgcgaact ggtggaaagc gttcccgcaa tttcgcgcgc gccgctggta
      841 cgcggtcgcg ctgggcagtc aactgcgatt tctcgccctc gacagcaacg gttcgctcct
      901 gcccgggagc gagcaggcgc agtggctgca ggaccagatc caggcactac ctgcgagcgt
      961 acgctttgtg ttcatcctgt tgcaccatcc gctgatgacc gacggtcacg agggggtacg
     1021 cgcaaacgag gcggccctgg ccgctcaatt ggcggccgtg gcaccgcacg cgccagcgcg
     1081 ttttgtcgtc tgtaccgcac atgtacacaa ttacgagcgt tttgagcgcg atggcgtggt
     1141 attcctggtg tccggtggcg gtggtgcgaa gcccacaccc atcacgcgct caagcgccga
     1201 tcgttatcgg aaggatgact ttccaaactt tcattacatt cgcttcgagc taacctcgcg
     1261 cggacttcgg ggcgagatgg tgcggctatc ggatgccgaa tcgacgaatc tggtcacgtg
     1321 ggcggtcaat gatcgattcg aaatcgacca gtttcgtacc accaggacaa tcgattagac
     1381 tgatgtcgat gattttttat tgctgcgacc tctgctcttt agctagcgac cagttgccga
     1441 tcacatacaa gagcccattg ctgtctcggc ggctgccttc gaactgagga taagtttggt
     1501 gcgtcgggtt gcaagaacgc cgacaagctt cttgatgcgc gcggtgcgct ccgactccgc
     1561 actctgtctt acttcacctt aaacggagag tattcgtgcg gcgcgcagcg gtgcatttcg
     1621 cgtaccgacg gcgggtattt cccgccgaga tcggtgatac cgtaatgccg acccagctct
     1681 gcaccaatga gcgtcttgcc gctgacctcc acaagcttgg tatctttgaa cagagcccag
     1741 atcacatggc cggtgaattc ggtcgattcg agcgtgcctt ccaggtgcct gaacttattg
     1801 ggtgccgcct gcatcagctg caccagccgt tccgacatca acgatcccat ccagatcgaa
     1861 accgcagcca cattcacgcc tgcgtccttg aagtccaccg ccatatcgaa agccatcttg
     1921 tctacgccgg ccttatgtgc gccataagca ggaccaagac agtagtaaac cgagccaggt
     1981 gctgaggtaa atacaatcag tccgctgcct tgctttacca tcgtcggtgc gacaagccag
     2041 ctggcggcga acccactacg cacgccgaca ttaatcatat tgccgatctc gagcggtttc
     2101 tcccaaaagt tgcccgggcc gggcaagcga tcgtgaatcg cgcaggcgtt gttcacgaga
     2161 atgtcgatcc gtccctgctc gcgcgtgacc tgcccgatca tggccttgac ctgcgcatcg
     2221 tcggcgtgat cgcagcgcac ggcgatgcct ttgccacccg ccttcgtcac cgcctcggcc
     2281 gtcgcataga tcgtgcccgg catcgaggca tctccttctc gttgcgaacg accggtgaca
     2341 tagacaacgc agccatgaga accgagcgct atcgcaatgc cttttccggc gccgcgactg
     2401 gcgcctgtga caattgccac gggtggcgaa tgagtcattg agcatttcct ggttacggca
     2461 gtctgaatgc aggac
//
LOCUS       DATMRM010000008         9617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1242011, whole genome shotgun sequence.
ACCESSION   DATMRM010000008 DATMRM010000000
VERSION     DATMRM010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9617
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1242011"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VNZ06_00235"
     CDS             complement(<1..667)
                     /locus_tag="VNZ06_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWW19203.1"
                     /translation="MRLVPLAGLLLTATLGGCVHVPTVPDSLPTEAPAQIAVHDARGR
                     LSTRETDALLKRIAAQSPDADAMQRHLAIEQTLSGRPLYTGNRVTPLRDGPDTFAATF
                     EAIHQAQHYLYLEYYILEDVECNGERLSDLLIQRQQQGVQIDVLYDTLGSLSTPKEFF
                     EQLSGAGIHVQPFNPFSPMSPFSSLNSRDHRKILVADSELAIIGGVNLSTDYQSLGSS
                     HAAA"
     gene            781..1230
                     /locus_tag="VNZ06_00240"
     CDS             781..1230
                     /locus_tag="VNZ06_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWW19204.1"
                     /translation="MEETHLAEGVQRREDPALRILIGENNSDLAMTLSLLLDAEPDMH
                     CVSTVASGRAVLHAVDEYAPNAFILDLSLDDGSSLPLIGILRQRLPHAVIVVFTGHRN
                     ELLSEQCVRAGANSVVVKTGEFDELTTALRSAAQRGWSPPGAKRADG"
     gene            1376..1837
                     /gene="rimP"
                     /locus_tag="VNZ06_00245"
     CDS             1376..1837
                     /gene="rimP"
                     /locus_tag="VNZ06_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HWW19205.1"
                     /translation="MTVPLRDRLIALTEPLLAQLGYELVDLEYVPGRAQAVLRVFIDR
                     PEGVGLDDCERASHEISALLDVQDPVPVAYTLEVSSPGLDRVLRTPAHFQRFVGERIW
                     LELAAPREGRRRYTGRLEGLSGEGIELNVDGAMVRVPFAEIGRARLAPLWT"
     gene            1854..3350
                     /gene="nusA"
                     /locus_tag="VNZ06_00250"
     CDS             1854..3350
                     /gene="nusA"
                     /locus_tag="VNZ06_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003235027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HWW19206.1"
                     /translation="MNKEILMVVDAVSNEKGVDKENIFEALEAALASATRKKHGEDWD
                     VRVSINRKTGDYETFRRWKVFADDSKELENPSAELRMDDAKDLSVDAEPGGFVEQPME
                     SVAFGRIAAQQAKQVIVQKVREAERAQVVDAYKDRVSTLVNGVVKRIDRNGIYVDLGG
                     NAEGFVPRSDMIPREQLKPQDRVKAYLREVRAEPRGPQLFLTRTAPEFLMELFKLEVP
                     EVGQGLIQILGAARDPGIRAKIAVRSQDPRIDPVGACVGMRGSRVQAVSNEIAGERVD
                     IIPHDENAAQFVINAMSPAEVQSIVVDEDSHSMDIAVAEEKLSQAIGRGGQNIRLASQ
                     LTGWELNVMTESDAEAKSESEARALVESFMKQLDVDENVANILVQEGFSTVEEVAYVP
                     AAELAAIEEFDEDIVKELRNRARDVLLTQAIVSEESLDQSMPSDDLLTLEGMRPDLAL
                     ALARRGVRTRDELAEQAVDDISDIEGLSHDEAGSLIMKARAHWFEAQH"
     gene            3372..5966
                     /gene="infB"
                     /locus_tag="VNZ06_00255"
     CDS             3372..5966
                     /gene="infB"
                     /locus_tag="VNZ06_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWW19207.1"
                     /translation="MADVTIAQFADVLKVPVDRLIAQLDQAGIAVSGPQDMISEDAKL
                     ELLTHLRRSHGHGAGAAEGSAPSRITLKRKTQSELKLAGNQGRARTVNVEVRQKRTYI
                     KRDVLEGQARAQQEQLDAQKREAEEAQRAQTERAEAERRERERLEAENRRRVEEEQAR
                     KKQAEESRRLAEQRAAEDAERERQKKAAAAAAAAEPAREPREARESHAPAAPHAPHAA
                     HAPHAPQPAARDDAESRTRYGREELHIAGDVSARHKKKKKLKSRTMPVSGSEAKHGFE
                     MPTAPVKREVALGETITVAELAQKMAVKATEVIKILMNLGVMATINQPIERDTAMLVV
                     EEMGHTAKVLRENQIEEGLQGDDSAEESETRAPVVTVMGHVDHGKTSLLDRIRTTKVA
                     AGEAGGITQHIGAYHVDTPRGSITFLDTPGHAAFTAMRARGAKATDVVVLVVAADDGV
                     MPQTIEAIQHAKAAGVPIVVAVNKIDKPDADPERVRTELTKHEVISEQWGGENIFVNV
                     SARTGEGIDALLEAILLQSEVLELKAPRSGLASGVVIESSMEKGRGAVVTVLIKRGTL
                     RAGDAILAGAEFGRVRALFDESGKAIDEAPPSTPVVVLGLSGAPNAGEELLVVENERK
                     AREVALYRQGKFRDVKLAKASTRAEDVFSQMGETKAGVIAVLIKTDVQGSAEALRDSL
                     VKLSTDEVKVSVIASGVGGITASDIQLAAASKAFVIGFNVRADSAARDAVKETGVEVR
                     YYSVIYEAIDDVKQKMSGLLAPEIKEQIVGTAQVREVFRSSKFGVVAGCLVVEGVVKR
                     NNPIRVLRDNVVIFEGALESLRRFKDDVGEVRAGTECGIGVKNYQDVRVNDQIECYSR
                     VEVSRTIQ"
     gene            5988..6365
                     /gene="rbfA"
                     /locus_tag="VNZ06_00260"
     CDS             5988..6365
                     /gene="rbfA"
                     /locus_tag="VNZ06_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWW19208.1"
                     /translation="MQKASARTSRIASQVQRTLAELLRRGVKDPRVGNLTITAVEVAN
                     DLSFARVHVLPFAASQPAPDLLAGLRSAAGYLRGELARELKLRHMPRLDFQLDQVIEE
                     AHRLNGLIDSAVKSDRARHDEDT"
     gene            6362..7252
                     /gene="truB"
                     /locus_tag="VNZ06_00265"
     CDS             6362..7252
                     /gene="truB"
                     /locus_tag="VNZ06_00265"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HWW19209.1"
                     /translation="MSAAVAGVLLLDKPVGLSSNAALQTVRRLAGGIKAGHTGSLDPL
                     ASGMLPICLGEATKLAGEMLCHSKCYRFGVQLGERSETGDAQGAIIERRAVPAFRSEQ
                     LSAVLARFCGVQQQIPPMYSALKRQGEPLYRLARRGLQVEREPRSVTIEALELLGREP
                     ERLQLRVLCSKGTYVRVLGEDIAQALGTCGRLYELRREFVQPFGDQPMATLQDLQAAG
                     SPSEWPILPADRAVSHLPALHLDLAGVKALAHGKVLAAAAQQCGTCRLYDPDGRFVGL
                     GQADEALSLRVLRLFAAAQQ"
     gene            7350..7619
                     /gene="rpsO"
                     /locus_tag="VNZ06_00270"
     CDS             7350..7619
                     /gene="rpsO"
                     /locus_tag="VNZ06_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWW19210.1"
                     /translation="MALSTEQKSGVVAQYRRGPSDTGSPEVQIALLSERINGLGEHFN
                     QHKRDHSSRRGLVKLVNQRRKLLDYLKATTPSKYQEVVERLGLRR"
     gene            7697..>9617
                     /gene="pnp"
                     /locus_tag="VNZ06_00275"
     CDS             7697..>9617
                     /gene="pnp"
                     /locus_tag="VNZ06_00275"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017391359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWW19211.1"
                     /translation="MTAFKKTFAYGPHSVTLETGELARQADGAVLASMGETVVLVTAV
                     AQKTSRADRDFFPLTVNYIEKTYAAGRIPGGFFKREGRPSERETLTSRLIDRPIRPLF
                     PDGFFNEVQVVATVLSLNPDIDADIPALLGASAALLLAGVPFNGPIGAARVGYKDGNY
                     LLNPTRTDQATSQLDLVVAGTEPAVLMVESEAQGLSEETMLGAVVFGHQQMQVAIAAI
                     RELAKEAGKPRWDWKPAATLAALEQAVAGKAESALAQAYRITEKQQRVARVAEIKKEV
                     STALIADEASGFSAEGVGDALFRLESRIVRERILAGEPRIDGRDMKTVRPITVKVGVL
                     PRTHGSALFTRGETQAIVTTTLGTTRDAQIIDALEGERREPFMMHYNFPPFSVGETGM
                     MGSPKRREIGHGNLARRGVSAVMPDMETFPYVIRVVSEILESNGSSSMASVCGASLSM
                     MDAGVPIKAPVAGVAMGLVLEGSRFQVLTDILGDEDHLGDMDFKVAGTRDGVTALQMD
                     IKVEGITPEIMRVALNQARAGRLHILDEMAKVIREPRSKMSEWAPSIITLKIDPEKIR
                     DVIGKGGSVIRQITEETGTTIDIENDGTVKIASVNGTAGREAQRRIELITADVEVGRV
                     YEGRVAKLMDFGAFVT"
BASE COUNT         1814 a         2907 c         3320 g         1576 t
ORIGIN      
        1 gcgcggcggc gtggctgctg ccgaggctct gatagtcggt gctcagattc accccgccga
       61 tgatcgccag ttcgctgtcg gccaccagga tcttgcggtg atcgcggctg ttgagcgacg
      121 aaaacggcga catcggactg aaaggattga acggctgaac gtgaattccg gcgccgctga
      181 gctgctcgaa gaactccttc ggtgtggaca gcgagccgag cgtgtcgtac agcacgtcga
      241 tctgcactcc ctgctgctga cgctggatca gcagatcact cagccgctcg ccgttgcatt
      301 ccacgtcctc gaggatgtag tactcgagat acaggtagtg ctgcgcctgg tgaatggcct
      361 cgaaggtggc ggcgaaggta tccggtccgt cgcgcagcgg agtcacgcgg ttcccggtgt
      421 agagcgggcg tcccgagagg gtctgctcga tcgcgagatg gcgctgcatc gcgtccgcat
      481 caggggactg cgccgcgatg cgcttgagca gcgcatcggt ctcgcgcgtg gacagtcggc
      541 cgcgcgcgtc gtgcaccgcg atttgcgccg gcgcctcggt gggcagcgaa tccgggacgg
      601 tcggaacatg cacgcagccg ccgagcgtcg cggtgagcaa caggcccgcg aggggaacaa
      661 ggcgcaaacc catccgctac tgtgcggtga tcgcgccgcg ggctcaagcc tgtcagacgt
      721 cgctgacagg acgccacgcg ggcgtttgca gcgacaaggc gaaggcgcga tgattccgac
      781 atggaagaaa cgcatctcgc cgaaggcgtg cagcgacggg aggatccggc gctgcgtatc
      841 ctgatcggcg agaacaactc cgacctggcg atgacactga gcctgctgct cgacgcggag
      901 ccggacatgc attgcgtgtc caccgtcgcc tcgggcaggg cggtgctgca cgccgtagat
      961 gagtatgctc cgaatgcttt cattctggat ttgtcgctgg atgacggctc gagcctgccg
     1021 ctgatcggca tcctgcggca gcgcctgccg catgccgtga tcgtggtgtt caccgggcac
     1081 cgcaacgagt tgctgagcga gcagtgcgtg cgggccggcg cgaacagcgt ggtcgtcaag
     1141 accggggaat tcgatgagct gaccacggct ctgcgcagcg ccgcacagcg cggttggtcg
     1201 ccgccgggag ccaagcgcgc agatggttga atttctttga cctaaagcac tagaatcgcg
     1261 ccgtccggtg gacggtgtca ggctctgact gacttgccgt aaagccgcag atggtgcggc
     1321 caagtgcttg atctagaagc cccacatggg gcttttcttt ttttgggcac tcatcatgac
     1381 agtaccgctg cgcgacaggc tgattgcgct gaccgagccg ctgctcgctc agctggggta
     1441 cgagctggta gatctggagt acgtgccagg acgagcacag gcggtgctgc gggtatttat
     1501 cgaccggccc gaaggggtgg gcctcgatga ctgcgagcgc gcaagtcatg agatttcggc
     1561 cttgctggat gtccaggatc cggtgccggt ggcatacacg ctggaggtgt catcgccggg
     1621 gctggatcgg gtactgcgca cgccggcgca ttttcagcgc tttgtcgggg aacgcatctg
     1681 gctggaattg gccgcccccc gcgagggtcg acgccgctat accgggcggc tcgaggggct
     1741 cagcggcgag ggcattgaat tgaatgtgga tggcgccatg gtgagggtgc cgtttgcgga
     1801 aatcggccgc gcgcggctgg caccgctgtg gacttaaggg gtctaagaag caaatgaaca
     1861 aggaaattct gatggtcgtg gacgccgtct cgaacgagaa gggcgtcgac aaggagaaca
     1921 tcttcgaggc gctggaggcg gcgttggcct cggctacccg caagaaacac ggcgaggact
     1981 gggacgtgcg cgtctcgatc aatcgcaaga ccggcgacta cgagacgttc cggcgctgga
     2041 aggtatttgc cgatgattcc aaggagctcg agaacccctc ggccgagctg cgcatggatg
     2101 atgcgaagga cctgagcgtg gatgcggagc cgggtggctt cgtggagcag ccgatggagt
     2161 cggtggcgtt cgggcgcatc gccgcacaac aggccaaaca ggtcatcgtg cagaaagtgc
     2221 gcgaggccga acgcgctcag gtggtcgatg cctataagga ccgcgtcagc acgctggtca
     2281 atggcgtggt gaagcgcatc gatcgcaacg gcatctatgt ggatctgggc ggcaacgccg
     2341 agggcttcgt gccccgcagc gacatgattc cgcgcgagca actcaagccg caggatcgcg
     2401 tcaaggctta cctgcgcgaa gtgcgtgccg agccgcgcgg cccgcagctg ttcctgacgc
     2461 gcaccgcgcc cgaattcctg atggaactgt tcaagctcga ggtgccggaa gtcggccagg
     2521 gcctgatcca gattctgggc gcggcccgcg acccgggcat ccgcgccaag atcgcggtgc
     2581 gcagtcagga tccgcgcatc gacccggtcg gtgcctgcgt cggtatgcgc ggttcgcgcg
     2641 tgcaggcggt ctccaacgag atcgcgggcg agcgcgtgga catcatcccg cacgacgaga
     2701 acgcggcaca gttcgtcatc aatgccatgt caccggccga ggtgcagtcg atcgtcgtgg
     2761 acgaggactc gcacagcatg gatatcgcgg tggccgaaga aaaactgtca caggcgatcg
     2821 gccgcggcgg tcagaacatc cgcctcgcaa gccagctcac cggttgggag ctgaatgtca
     2881 tgaccgagtc ggacgcggag gccaagagcg agtcggaggc ccgtgcgctg gtcgagagtt
     2941 tcatgaagca gctcgacgtg gacgagaacg tggccaacat cctggtgcag gaaggcttct
     3001 cgacggtgga agaagtggcc tatgttccgg cggccgagct ggcggcgatc gaggaattcg
     3061 acgaggacat cgtcaaagag ctgcgcaacc gcgcccgcga cgtgctgctg acccaggcga
     3121 tcgtcagcga agaatcgctc gatcagtcga tgccgagcga cgatctgctc acgctcgagg
     3181 gaatgcggcc ggatctggca ttggcgctgg cacggcgcgg cgtacgcacg cgcgatgaac
     3241 tcgccgagca ggcggtggat gacatttccg acatcgaggg cctgtcgcac gacgaggccg
     3301 gcagtctgat catgaaggcg cgcgctcact ggttcgaggc acagcactga ggctgtcgca
     3361 ggtgaagaca tatggctgat gtgaccattg ctcaatttgc cgatgtgctg aaggtcccgg
     3421 tggatcggct gatcgcacag ctcgatcagg ccggcatcgc ggtttccgga cctcaggaca
     3481 tgatcagcga ggatgcgaaa ctcgagctgc tgacgcatct gcgccgcagc catggtcacg
     3541 gtgccggtgc ggccgagggc tcggccccca gtcgcatcac gctcaagcgc aagacccaga
     3601 gcgaactcaa gctcgctggc aatcaggggc gtgcgcgtac cgtcaacgtc gaagtgcggc
     3661 agaagcgcac ctacatcaag cgggacgtgc tggagggcca ggcgcgcgcg cagcaggaac
     3721 agctggatgc gcagaagcgc gaggccgaag aggcgcagcg cgcgcagacc gagcgggccg
     3781 aagccgagcg ccgcgagcgc gagcgcctcg aggccgagaa ccggcgccgc gtcgaggaag
     3841 agcaggcccg caagaagcag gctgaagaga gccgccgcct cgccgaacag cgcgccgccg
     3901 aagacgccga gcgggaacgg cagaagaagg cggccgccgc ggccgccgcg gccgagccgg
     3961 cgcgcgagcc acgcgaggcc cgcgaatcgc atgcgcctgc ggcaccgcac gcgcctcatg
     4021 cagcgcatgc gccccatgcc ccgcagccgg ccgcgcgcga tgatgccgag tcacgcaccc
     4081 gctacggacg cgaagagctg catattgccg gtgacgtcag cgcacgtcac aagaagaaga
     4141 aaaaactcaa gtcgcgcacc atgccggtca gcggcagcga ggccaaacac ggctttgaaa
     4201 tgccgaccgc gccggtcaag cgcgaagtcg cgctgggcga gacgatcacg gtggccgagc
     4261 tggcgcagaa gatggcggtc aaggccaccg aggtcatcaa gattctgatg aacctcggtg
     4321 tgatggcgac catcaatcag ccgatcgaac gcgataccgc gatgctggtg gtcgaggaga
     4381 tgggccacac cgcgaaagtg ctgcgcgaga accagattga agaaggattg cagggtgacg
     4441 acagcgccga ggaaagtgaa acgcgcgcac cggttgtgac agtgatgggc catgttgatc
     4501 acggtaaaac ctcgctgctc gatcgcattc gtaccaccaa agtggctgcg ggcgaggccg
     4561 gcggaattac tcagcacatt ggcgcctatc acgtcgacac accgcgcggc tcgatcactt
     4621 tcctcgatac gcccggtcac gcagcgttca ccgcgatgcg cgcgcgcggc gcgaaggcca
     4681 ccgacgtcgt ggtgctggtg gtggcggcgg acgatggcgt gatgccgcag accatcgagg
     4741 cgattcagca tgcgaaagcc gctggcgtgc cgatcgtggt cgcggtcaac aagatcgaca
     4801 aacccgatgc cgatccggag cgcgtgcgca ccgaactgac caagcacgag gtcatttccg
     4861 agcagtgggg cggtgaaaat attttcgtga acgtctcggc tcgcaccggc gagggcatcg
     4921 atgcgctgct ggaggcgatc ctgctgcagt cggaagtact ggagctgaag gcgccgcgca
     4981 gcggactggc ctccggcgtc gtcatcgagt cgagcatgga gaaaggtcgc ggcgcggtgg
     5041 taaccgtgct gatcaagcgc ggcacgttgc gtgccggcga tgcgatcctg gccggtgccg
     5101 agttcggtcg tgtgcgagcg ttgttcgatg agagcggcaa agcgatcgac gaggcgccgc
     5161 cctccacacc ggtcgtcgtg ctgggattgt cgggcgcacc gaacgccggc gaggaactgc
     5221 tggtcgtgga gaacgagcgc aaggcgcgcg aagtcgcgct ctatcggcaa ggcaagttcc
     5281 gcgacgtcaa gctggccaag gccagcaccc gtgccgagga cgtgttctcg cagatgggcg
     5341 agaccaaggc cggcgtgatc gcggtgctca tcaagacgga cgtacagggc agcgccgagg
     5401 cgctgcgcga ctcattggtc aagctgtcca ccgacgaggt caaggtcagc gtgatcgcca
     5461 gcggtgtcgg cggcatcacc gcctcggaca tccagcttgc agccgcctcc aaggcgttcg
     5521 tcatcggctt caacgtgcgc gcggattccg cggcacgcga cgcggtgaag gagaccggag
     5581 tcgaagtgcg ctactacagc gtcatctatg aggcgatcga cgacgtcaaa cagaagatga
     5641 gcggcctgct cgcaccggag atcaaggaac agatcgtcgg taccgcgcag gtgcgcgagg
     5701 tgttccgctc cagcaaattc ggtgtcgtgg ccggctgcct ggtggtcgaa ggcgtcgtca
     5761 agcgcaacaa cccgatccgt gtgttgcgcg acaacgtcgt gatcttcgaa ggcgcgctcg
     5821 aatcgctgcg gcgcttcaag gacgacgtcg gcgaagtgcg tgccggcacc gagtgcggta
     5881 tcggcgtcaa gaactatcag gacgtgcgcg tcaacgatca gatcgagtgt tattcgcgcg
     5941 tcgaggtgtc gcgcacgatc caataggcgg atcgtgtcga ggattgcatg cagaaggctt
     6001 cggcccgcac ctcacgcatt gcttcgcagg ttcagcgcac gctggccgaa ctgctgcgcc
     6061 ggggcgtgaa ggatccgcgc gtcggcaacc tcaccatcac tgcggtggaa gtggccaacg
     6121 acctgagttt cgcgcgagtg cacgtgctgc cgtttgccgc ctcgcagccg gcgccggatc
     6181 tgctcgccgg gctgcgcagc gccgccgggt atctgcgcgg tgagctggcg cgcgaactga
     6241 aactgcgtca catgcctcgc ctggattttc agctcgacca ggtgatcgag gaggcgcacc
     6301 gtctcaacgg cctgatcgac agcgccgtca aatccgaccg cgcccggcac gacgaggaca
     6361 cttgagcgcc gccgtagccg gcgtgctgct gctcgacaaa cccgttgggc tgtcctcgaa
     6421 cgcggcactg cagacggtgc ggcgcctcgc cggcggcatc aaagccggtc acaccggcag
     6481 ccttgatccg ctcgccagcg gcatgctgcc gatctgcctg ggggaagcca ccaaactggc
     6541 cggcgagatg ctctgccaca gcaagtgcta tcgcttcggc gtgcagctcg gtgagcgttc
     6601 cgaaaccggc gatgcgcaag gcgcgatcat cgagcgccgg gcggtgccgg cgtttcgcag
     6661 cgagcagctc agcgcggtgc tggcacgctt ttgcggtgta cagcagcaga ttcctccgat
     6721 gtattccgcg ctcaagcgcc agggcgagcc gctgtaccgg ctggcgcgcc ggggcctgca
     6781 ggtggaacgc gaaccgcgca gcgtgacgat cgaggcactg gagttgttgg ggcgcgaacc
     6841 cgagcggctg caactgcggg tgctgtgctc gaaaggtact tatgtccggg tgctgggcga
     6901 agacatcgcg caggcgctcg ggacctgcgg ccggctgtat gagctgcgcc gcgagttcgt
     6961 gcagcccttc ggcgatcagc cgatggcgac cctgcaggat ctgcaggccg ccggcagccc
     7021 gagcgagtgg ccgatcctgc cggccgaccg cgcggtctcg catctgccgg cgctgcatct
     7081 ggacctcgcg ggcgtcaaag cgctggcgca cggcaaggtg cttgcggcag cggcgcagca
     7141 gtgcggaacc tgccggctgt acgatccgga tggccggttc gtcgggctgg ggcaggcgga
     7201 tgaggcgctt agcctgcggg tactgcgact attcgctgcc gcacagcagt aactccctgt
     7261 tcggcttgag acaagcaggg tctgcggtta gaatgcgcga cctttaaaac gacccatgca
     7321 gtcaagtagt cagaaggatt ttcacggcaa tggctctgtc gacggaacaa aagagcggtg
     7381 tggtggcgca gtaccgccgc ggtccgagcg ataccggatc gcccgaagtg cagattgcac
     7441 tgctgtccga gcgcatcaac ggactgggcg aacacttcaa tcagcacaag cgcgatcact
     7501 cttcgcgtcg cgggctggtc aaactggtga atcagcgccg caagctgttg gactatctca
     7561 aggcgaccac accctccaag tatcaggaag tggtcgagcg cctggggctc cgccgctgac
     7621 cacgaaaagg ccgcgcaact cacccgaacg cgcggccttc tactatctgc accactattt
     7681 ttccgaggat tgaagcgtga ctgccttcaa gaagactttt gcctatggcc cccattccgt
     7741 caccttagag accggcgaac tggcgcgcca ggccgatggt gccgtccttg cctcgatggg
     7801 cgagaccgtc gttctggtga ccgcggtggc ccagaaaacc tcacgggccg atcgtgattt
     7861 ctttccgctg accgtcaact acatcgagaa gacctacgca gccgggcgca ttcccggcgg
     7921 cttcttcaaa cgcgagggac gtccttccga gcgcgagacg ctgacctcgc gtctgatcga
     7981 tcgcccgatt cgcccgctgt tcccggacgg cttcttcaac gaagtgcagg tcgtggccac
     8041 ggtcctgtcg ctcaatcccg atatcgatgc cgacattcca gcgctgctgg gagcctcggc
     8101 cgcgctgctg ctggccgggg tgccgttcaa tggtccgatc ggcgcggcgc gcgtcggcta
     8161 caaggacggc aactatctgc tcaatccgac acgtaccgat caggccacct cgcagctcga
     8221 tctggtggtc gctggcaccg agccagcggt gctgatggtg gagtccgaag cgcaggggct
     8281 gtccgaggag acgatgctcg gcgcggtggt cttcggccac cagcagatgc aggtcgcgat
     8341 cgcggcgatc cgcgagctgg ccaaagaggc tggcaagcct cgctgggact ggaagcccgc
     8401 ggcaaccctc gcggcgctcg aacaggcagt ggcgggcaaa gccgaaagcg ctctggccca
     8461 ggcctatcgc atcactgaga agcagcagcg cgtggcgcgt gttgccgaga tcaagaagga
     8521 agtcagcacc gcgctgatcg cggatgaggc cagtggcttc agcgccgaag gcgtcggcga
     8581 cgccttgttc cggctcgaga gccgcatcgt gcgtgagcgc attctggctg gcgaaccgcg
     8641 tatcgatggt cgcgatatga agacggtgcg accgattacg gtcaaggtcg gcgtgttgcc
     8701 gcgtacccat ggttcggcac tgtttacccg cggagagacc caggcgatcg tcacgaccac
     8761 gctcggcacc acccgcgatg cgcagatcat cgatgccctg gaaggcgagc ggcgcgagcc
     8821 cttcatgatg cattacaact tcccgccgtt ctcggtgggt gagaccggaa tgatgggctc
     8881 gcccaagcgc cgcgaaatcg gccacggcaa tctcgcccgg cgcggcgtga gtgcggtgat
     8941 gcccgacatg gagaccttcc cgtatgtcat ccgtgtggta tcggagattc tggagtccaa
     9001 cggttccagt tcgatggcga gcgtgtgcgg tgccagcctg tcgatgatgg atgccggtgt
     9061 gccgatcaag gccccggtcg ccggtgttgc gatgggactg gtgctcgaag gcagccgctt
     9121 ccaggtgctg accgacattc tgggcgacga ggatcacctg ggcgacatgg atttcaaggt
     9181 agccggtacg cgcgatggcg tgacggcgct gcagatggac atcaaggtcg agggcatcac
     9241 gcccgagatc atgcgggtcg cgctcaatca ggcgcgtgcc ggccgactgc atatcctcga
     9301 tgaaatggcg aaagtgatcc gcgagccgcg ctcgaagatg tccgagtggg ccccctcgat
     9361 catcacgctg aagatcgacc cggagaagat tcgcgacgtc atcggcaagg gcggctcggt
     9421 gatccgccag atcaccgaag agaccggcac cacgatcgac atcgaaaacg atggcacggt
     9481 gaagatcgcg tcggtcaatg gtactgccgg ccgcgaggcg cagcggcgga tcgaattgat
     9541 cacggcggac gtcgaggtag gccgggtcta cgagggccgg gtcgcgaagc tgatggattt
     9601 cggcgcgttc gtcacga
//
LOCUS       DATMRM010000009         3365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3411553, whole genome shotgun sequence.
ACCESSION   DATMRM010000009 DATMRM010000000
VERSION     DATMRM010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3411553"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VNZ06_00280"
     CDS             complement(<1..184)
                     /locus_tag="VNZ06_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19212.1"
                     /translation="MASISRPPMMSKKMIIGRSHSFHRERRNAKSSIRKSSIALVPVT
                     SRRRMRKEFCHAAQVPP"
     gene            129..320
                     /locus_tag="VNZ06_00285"
     CDS             129..320
                     /locus_tag="VNZ06_00285"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19213.1"
                     /translation="MRPIIIFLLIIGGLLMLANGFLGGGGSPTVEDPVVPFSRSAYRH
                     DAAFFNRYSAMSLCPSRFG"
     gene            complement(251..1432)
                     /locus_tag="VNZ06_00290"
     CDS             complement(251..1432)
                     /locus_tag="VNZ06_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19214.1"
                     /translation="MLVRAHELNGKAAAPTWPKAVEERAMIPGHDRTLRRASPGREHA
                     RRFIENHQRSIVLGWMLLLLCLLLVGAELLLRYTLSYRIDYYTGDKISNRLIRFPFGD
                     MPFNSNGYPDREWDEEDPRIRVGFWGDSITSGVGAGFGYRYTDIISDTRRDRDYRNFG
                     GPGEDGIADDRAIEKILEIAQRFQLSKIVYAMDLNDILPDKGAPAARHSRLYKTKLWV
                     KQYLDVLRSRSYVYNYLRLKLSEAAVRMGYGYHGDEAFELHPLRNAVIVNQTVERINK
                     LSMRLKQHAVDLCVVVFPYEMQISADAAARYQQDGVRWSTELLRGEPQQMILGRLARD
                     IVAVDLAPAFHQSRDGPASIAVGEYFVFNKGDALDWFHPNRDGHRLIAEYLLKNAASC
                     L"
     gene            1415..2248
                     /locus_tag="VNZ06_00295"
     CDS             1415..2248
                     /locus_tag="VNZ06_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HWW19215.1"
                     /translation="MRTDEHNSNEALNGGRGQALIEALVIALAVAATALLCVYHLRKV
                     RLAEFARRATLGSRSQRDAAFALATAIFGEVKRGRDPAFLLPLLAPLGGSPTTILKKG
                     GCCSGVHRLFITSLDALGIRAAQVVVYRRADPAAAHCLVQVIAEGTNLLIDADYGVWY
                     CHPDGGPIDLAGLRSGLRPAIVPFVLDRQAGYVDSSRTRSAGYPDREYYRFDYDLTRT
                     ANWAESRLKRACYAVLHPLTRGRVDYLLLPAILEWPEVLLAAALCGATILLLAARALT
                     H"
     gene            2458..3180
                     /locus_tag="VNZ06_00300"
     CDS             2458..3180
                     /locus_tag="VNZ06_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19216.1"
                     /translation="MRTKISKAVRPRAPLALAKLLDELERFYGPQQPSWPLDPYAFIV
                     WWQCGYPPSDAACERGWDKLRSDAGSELHALLQTSTAKLASLLKSGGLVPEVRALRLK
                     QIAKRVQNELGGDLLGALRAPLCEARKVLKKFPGIAEPGADRILLFARIAPIAAVPSN
                     CPHVLSRILHGREQDTYRANYRDAQERIAAEVAESFDARIRAYLLLKRHGQELCKRTQ
                     PLCQECPLRRACLFAAAQRAAP"
BASE COUNT          599 a         1101 c         1002 g          663 t
ORIGIN      
        1 gtggtggcac ttgtgcagca tggcaaaact ccttgcgcat ccgccgacga gatgtaacgg
       61 gaacaagagc gatgctggat tttcttattg aactcttcgc attcctgcgc tcccgatgga
      121 agctgtggct gcgtccgatt atcatttttt tgctcatcat cggcggcctg ctgatgctgg
      181 ccaacgggtt tctgggaggg ggaggttctc ccacagtcga ggacccggtt gttcccttct
      241 cgcgcagtgc ctacagacac gacgccgcgt ttttcaacag gtactcagcg atgagcctat
      301 gcccgtcgcg gttcggatga aaccagtcga gggcatctcc cttgttgaac acgaagtact
      361 cgcccactgc gatcgaggca ggtccgtcac ggctttgatg gaacgccggc gcaaggtcga
      421 cggcgacaat atcgcgcgcc agtcgtccca gaatcatctg ttgcggctcg ccgcgcagca
      481 gctcagttga ccagcgcacg ccgtcctgtt ggtaacgtgc ggctgcgtcg gcagatatct
      541 gcatttcata cggaaacacc acgacacaca aatccaccgc atgctgtttc aacctcatcg
      601 ataacttatt gatccgctcg acggtctgat tcacgatcac ggcgttgcgt agcgggtgca
      661 gttcaaacgc ctcgtcaccg tgatatccat agcccattcg cactgcagcc tcgctcaact
      721 tgagccgtag gtagttgtat acgtaagagc ggctcctgag gacgtccagg tattgtttaa
      781 cccagagctt tgtcttgtat agtcgcgagt gtcgagccgc cggtgccccc ttgtccggca
      841 ggatgtcatt gaggtccatc gcgtagacga tcttgctcag ctggaaacgt tgagcgattt
      901 ccaggatctt ctctatcgcg cgatcatcgg cgataccgtc ctcacctggg ccaccgaaat
      961 tcctgtagtc gcggtcccgg cgagtgtcgc tgatgatgtc ggtgtagcga taaccaaagc
     1021 cggcgccgac accgctcgtt atggaatcac cccagaaccc gacgcgaatc cgagggtcct
     1081 cctcgtccca ttcccgatcc ggataaccat tcgagttaaa tggcatgtct ccgaacggga
     1141 acctgatcag ccggttggag atcttgtcac ccgtgtagta gtcgatccga tacgagagcg
     1201 tgtatcgcag gagcagttct gcgcccacca gcagcaagca aagcaagagc agcatccaac
     1261 cgagcacgat gctgcgttga tggttctcga tgaagcgccg ggcatgctcg cggccggggc
     1321 ttgcccgtct gagggtacga tcgtggcccg ggatcatcgc gcgctcctca accgctttcg
     1381 gccaagtcgg ggccgctgcc tttccgttca attcatgcgc acggacgagc ataatagcaa
     1441 cgaggcactg aacgggggcc ggggtcaggc tttgatagag gcgctcgtca tcgcgctcgc
     1501 tgttgccgct acggcgctgc tttgcgtgta ccacctgcgc aaggtgcgcc tcgccgaatt
     1561 cgcgcggcgc gcaacgctcg gcagccgctc gcaacgcgat gctgcctttg cgctggccac
     1621 cgcgatattc ggcgaagtga agcgcggacg tgatccggcg ttcctgctac cgctgctcgc
     1681 gcccctcggc ggctcgccca ccacgatcct gaaaaagggc ggctgctgtt ccggtgtgca
     1741 tcgacttttc attacctctc tcgatgccct cggaatccgc gccgcgcagg tcgtcgttta
     1801 ccgccgtgcc gatccggccg ccgcacactg cctggtacag gtcatcgcgg agggcaccaa
     1861 cctcctcatc gatgccgact atggcgtgtg gtattgccac cccgacggcg gcccgattga
     1921 tcttgccggg ctgcgctcgg gcctgaggcc cgccatcgta cctttcgtgc tcgaccggca
     1981 agccggctat gtcgacagct cccggacgcg atcggcgggc taccccgatc gggagtacta
     2041 ccggttcgac tacgatctga cccgcacggc caactgggcc gagagccgtc ttaagcgcgc
     2101 atgctatgca gtgctacacc ccctgacacg cgggcgcgtg gattatctgc tgctgccggc
     2161 aatactcgag tggccggagg tgctgctcgc cgcggcgctg tgcggcgcga caatcctgct
     2221 gctcgcggca cgggcgctga cgcactagat cagtgtcgcc tgcaccgacc tcaccctgcg
     2281 tcgaggacgg catcggcaac gcactcccat tctgcctgcc gctcacccgc cacaccccgt
     2341 cagcccgaac gcgcaatccc ctgccggcac cgggagtagg caaggtattc cgtatccggg
     2401 cccggagctg acgagcgcgt cgcctgcatc tgtttgaggc gcacactctc gcttgccatg
     2461 cggaccaaaa tctccaaggc tgtccggccg cgcgcaccgc tcgcgctcgc caagttgctc
     2521 gatgagctcg agcgcttcta cggcccgcag cagccctcgt ggccgctcga tccttacgcc
     2581 ttcatcgtgt ggtggcagtg cggctatcca cccagcgatg ccgcgtgcga gcggggctgg
     2641 gacaagctca ggagcgacgc tggcagcgaa ctgcacgcct tgctgcagac gagcacggca
     2701 aagctggcgt cgcttctgaa gtctggcggt ctggtccccg aggtgcgcgc cctgcggctg
     2761 aagcagattg caaaacgcgt ccagaacgag ttaggcggtg atctgctcgg cgcgctgcgg
     2821 gcgccactgt gcgaggcgcg caaggtgctg aaaaagtttc ccgggattgc cgaacccgga
     2881 gccgaccgca tcctgctgtt tgcccggatt gcccccatcg cggcggttcc gtccaattgc
     2941 ccccacgtat tgagtcgcat tcttcacggt cgcgagcagg acacctatcg ggccaattac
     3001 cgtgatgcgc aggagaggat tgccgcagag gttgcggaga gcttcgatgc ccgcatccgc
     3061 gcctacctgc tgctgaagcg ccacggccaa gagctctgca aacgcaccca gccactttgc
     3121 caggagtgtc ccctgcgccg cgcgtgccta ttcgcggccg cgcaacgcgc agcaccgtga
     3181 cttcgaacaa ttcggccgcg tgggcaccta ctgcgcaggt tcgtgcatca gcgtgcgccc
     3241 gtagtcgctc agcaggatct gcaggccaag ataacccaaa aactgcgcat gcccgtcggc
     3301 gtcgcccctc acgctgcgcc cggccatgaa cagcaggatg aaggccgggt gcagcttgta
     3361 gcgca
//
LOCUS       DATMRM010000010         8514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_5290, whole genome shotgun sequence.
ACCESSION   DATMRM010000010 DATMRM010000000
VERSION     DATMRM010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5290"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VNZ06_00305"
     CDS             complement(<1..648)
                     /locus_tag="VNZ06_00305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWW19217.1"
                     /translation="MTNPVGRRELLAAAGAATLLGVLDACQGPMQRPRVSERLELPPL
                     HADISHINAITVCTRPFRPEGPRLDVEHRADKTIVHNYGHGGSGWSLSWGSSAIATEN
                     ALATGERDIAVIGCGALGLTSALSLQRAGAHVTIYARDLPPNVRSSLATGVWTPDSRI
                     CLAQHATPEFKRLWERMARRSWQTYQTLLGLPGEPVEFFDEYRLVDAAVGSRREPV"
     gene            741..1010
                     /locus_tag="VNZ06_00310"
     CDS             741..1010
                     /locus_tag="VNZ06_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19218.1"
                     /translation="MSEICQPHNLRKAAGQGPKPYGTRISLKPGDPFRKIMGADWHKV
                     HWYASAAERDAALLDMSRKHEYSRSGDKPALVFEKVENLAASRGL"
     gene            complement(1018..3249)
                     /locus_tag="VNZ06_00315"
     CDS             complement(1018..3249)
                     /locus_tag="VNZ06_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWW19219.1"
                     /translation="MHLAKTVALAALLLLSVSAGAQEPASESAVPPAAANPGRPRIGL
                     VLSGGGARGAAHIGVLKMLDRLHVPVDVIAGTSMGAVVGGLYASGMTGVEIEHAMAAL
                     NWQAAFRDRPPRTQLDYRRKEEDRQFLVNLPLGLQGRRLVIPKGLLQGQLLTETLRKM
                     TLPVARITDFDQLPTPFRAVATNLETGAKVVLGSGDLTTAMRASMSVPGVFAPVEDHN
                     QILVDGGLADNLPIDVARAMGVDVLIVVDAGFPLQPAKNLNSLPSITNQMLAILLRRN
                     IERDLAMLSPKDIVVTLQLGDFSSYDFPDTMKIVNAGETAVWSAEPRLSALEVSAEEY
                     QRYLLARRSVRDGLPRIDYVKVQPDSKTYQRQIDDLFGQFVGQTLDPAALEQQVKALY
                     GRGNLEMLDYQLDHDPFGHYGLDFSARRNSWGPNYLRFGVQLEDDFRGLTTFNAAGRL
                     DITELNSLGAESVWDAQVGSAPLLATELYLPLSNVYRYFAAPHLQVEAHDIAQVEDGR
                     QVGLFRTSSFDYGIDFGREFSSWGEVRLGVLQSNGNTHVSLGDFSVKATHFDVTEGFV
                     RLGYDTLDSANFPHSGQALVAQVSIEGGAQGTDLFTVDWRAAHSWARNTVVAWLTAGS
                     TVGGSQTNVRTFFPLGGFLNLSGVQTGTLAGPQYAIGRLIYLRKVGNGGEGILDVPAY
                     VGTSFEIGNVWPDRRQISLSDTRQDFSIFFGADTYVGPAYFAIGYDASGSTSFFLFLG
                     RSF"
     gene            3362..4114
                     /locus_tag="VNZ06_00320"
     CDS             3362..4114
                     /locus_tag="VNZ06_00320"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWW19220.1"
                     /translation="MGSYLGLSIASIIIQIGLIVHCIRTGRSFLWVWVLLALPGVGSI
                     AYVLVEILPGLLGSRGARRAARGVSRALNPEQDLRRYEAEARHTGDVASRQRYADELL
                     RHDRAGDAVEVYKQTLKGLYEHDPNLMLGLARAQFANGAFAEARSTLDALIEHNPDFK
                     SPDGHLLYARALEGEGNRDKALEEYAAVAGYYAGAEAKLRYARLLSSLGKKEDSRRIL
                     KELLEHARFAPRHYRKMQQQWLADAERELASL"
     gene            complement(4111..5217)
                     /gene="aguA"
                     /locus_tag="VNZ06_00325"
     CDS             complement(4111..5217)
                     /gene="aguA"
                     /locus_tag="VNZ06_00325"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF010070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HWW19221.1"
                     /translation="MSRTLRSNPLRDGYWMPAEFEPHEGTWMLWPERPDNWRRNAQPA
                     QQAFAAVATAISQFEPVTIGASARQFARARAAMPADSVRLVELSSDDAWMRDTGPSFV
                     VNAAGAMRGVHWRFNAWGGIYNDSEHDNQVAQKVLELTGLERYHAPFVNEGGAIHVDG
                     QGTVLVTEQCLLNRNRNPGLKRAQIETLLRRYLGVTCVIWLGEGVINDETSGHIDNLA
                     CFARPGEVCLTWCDDPRDPQHRVSMDAFERLMAARDARGRRLRIHKLVSPGPLYMTRR
                     EAAGIVSDDSIRPLRAGTRLAASYANFYLANNAVILPLLDARTDAQVRQQLRRIFPRR
                     RIVGVAAREVLLGGGDLHCITQQQPRRQKRRAPF"
     gene            5317..6627
                     /gene="icd"
                     /locus_tag="VNZ06_00330"
     CDS             5317..6627
                     /gene="icd"
                     /locus_tag="VNZ06_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HWW19222.1"
                     /translation="MTNHPSSAKPIVPSAGQKITLKDGKLSVPDRPVVPFIEGDGTGR
                     DIWRASVRVLDAAVKKAYGARRQIEWMEVYAGEKAFNAFKSWLPDETVQACRDYLVSI
                     KGPLTTPVGGGIRSLNVALRQLLDLYVCLRPVRWFKGVPSPVKAPEKVDMVIFRENTE
                     DLYSGIEFEEGTEDNRKFLELLKTNFPKHYGKIRFPNTSGIGIKNISIEGTERIFRAA
                     IEYALRNRRKSLTIVHKGNILKFTEGSFRSWSYALAEREFGDRVYTWPQWERTKAASG
                     EKAANDEQAAALKAGKLLIKDAIADITLQQVLTRADEFDVVATMNLNGDYLSDALAAQ
                     VGGIGIAPGANINYLTGHAVFEATHGTAPKYADLDQVNPGSVILSGEMMLRYMGWTEA
                     ADAVISAMDKTIGQKIVTYDFARLMEGATKVKCSEFGDALIRNL"
     gene            complement(6662..8254)
                     /locus_tag="VNZ06_00335"
     CDS             complement(6662..8254)
                     /locus_tag="VNZ06_00335"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein D"
                     /protein_id="HWW19223.1"
                     /translation="MSRRRFLQAGAAVITGGLSLSETPAAEPAHPSPLKRLGQSEAFD
                     YARLKGLARAMAAAAYKPNNAPLPGPISKLSWDQWQSIRYRDDHSLWFGEGLRFQVRF
                     FHLGFTVTKPVRMYTVDKGQAQELAYDSAMFDYSRSGVDGRKLPANLGFAGFRLLFHT
                     DWVRDVAAFQGASYFRAVDSDKQYGMSQRGLAIDCGSTKPEEFPDFIAYYLERPANGS
                     SRLTVYALLDSPSVTGAYRFGIDVSDSIVTDIDAALYPRKTIERLGIAPGTSMFFYGS
                     NDRRASADWRPQVHDSDGLQINNGANEWLWRPLVNPAVVRVNSFLDDSPHGFGLMQRD
                     RDFDHYQDDGVFYDRRPSCWVEPRGNWGKGAVMLVEIPTVDETFDNVVAFWTPADKPQ
                     RGQEWLFAYRLYWCKYNPVSYPLAQVAATRDGIGGIVGQKHTYFSWRFVVDFAGGDIP
                     LLGEDARVEPVITASRGQVEITSARPLHSIHGWRAMFDLKPADDSTEPINLRLYLAVN
                     GQPLTETWIYQYTPPPPDQRKF"
BASE COUNT         1661 a         2710 c         2647 g         1496 t
ORIGIN      
        1 caccggctcg cgtctggagc cgacggctgc gtccaccagc ctgtattcat cgaagaactc
       61 caccggctcc ccgggcaggc cgagaagcgt ctgataggtc tgccatgaac gccgggccat
      121 ccgttcccac agccgcttga actccggggt cgcatgttgc gccaggcaga tacgcgaatc
      181 cggcgtccat acaccggtgg ccagcgagga gcgcacgttg ggaggcaaat cccgggcgta
      241 aatcgtcacg tgagcgccgg cgcgctgcaa cgacaaggcg gaagtgagcc ccagcgcgcc
      301 gcagccgatc accgcgatgt cgcgttctcc cgtggccagg gcgttctcgg tggcgatggc
      361 gcttgagccc cacgacaagg accagccgct gccgccgtga ccgtagttgt gaacgatcgt
      421 tttgtcagcg cgatgctcca cgtcaagccg cggaccttcg ggccgaaatg ggcgggtgca
      481 gaccgtgatc gcgttgatgt gactgatgtc agcatgcaat ggcggcagct cgagccgctc
      541 ggagacccgg gggcgctgca tcggaccctg acaggcatcc agtactccca gcagtgtggc
      601 ggcccctgcc gcggccagga gttctcgacg tccaaccgga tttgtcacgt tcctcccctc
      661 gcgtcccgct attgtttgca attgtatggc gatcgttccg gtttcggcga ggcaagcgcg
      721 cccgtagtaa aatcctttcc atgtccgaaa tctgtcagcc ccataatctg cgcaaggctg
      781 cgggtcaggg tcccaagccg tacggcaccc gcatcagcct caagcccggc gacccgttcc
      841 gtaagatcat gggagccgac tggcacaagg tgcattggta tgcgagcgcc gccgagcgcg
      901 atgccgcact gttggatatg agccgcaagc acgaatattc ccgctccggg gataaaccgg
      961 ccctggtgtt cgagaaggtg gagaacctgg cggccagccg cggcctgtga ggccgtttca
     1021 gaagctccgg cccaggaaca gaaagaacga ggtcgagccg ctggcatcgt agccgatcgc
     1081 aaaatacgcc ggcccgacat aggtatcggc gccaaagaaa atcgagaaat cctgccgggt
     1141 gtcgctcagg ctgatctgcc ggcgatccgg ccagacgttg ccgatctcaa acgaggtgcc
     1201 cacataggcc gggacgtcca gaatgccctc gccgccgttt ccgaccttgc gcagatagat
     1261 cagccgtccg atcgcgtatt gcgggccggc cagggttccg gtctgcaccc ctgaaagatt
     1321 cagaaagccg cccagcggga agaatgtgcg cacattggtc tggctgccac ccaccgtgct
     1381 gcctgccgtc agccaggcga ccaccgtatt gcgagcccag gagtgtgcgg cacgccagtc
     1441 gaccgtaaac aggtcggtgc cctgggctcc gccctcgatg ctgacctgtg cgaccagcgc
     1501 ctgaccggaa tgcgggaagt tggcactatc gagtgtgtcg tagcctaagc gcacgaagcc
     1561 ctcggtgacg tcgaagtggg tcgccttcac cgaaaaatcc ccgaggctca catgcgtgtt
     1621 gccgttgctc tgaagtacgc ccagtcgcac ctcgccccag ctgctgaatt cgcggccgaa
     1681 atcgatcccg taatcgaagc tggaggtacg gaacaggcca acctggcggc cgtcttcgac
     1741 ctgcgcaatg tcgtgtgctt cgacctgcag atgcggcgct gcgaagtagc ggtagacgtt
     1801 ggacagcggc aggtagagtt ccgtcgccag caacggcgcg ctgcccacct gagcgtccca
     1861 caccgactcg gcaccgagcg aattgagctc ggtgatgtcc agacggcccg ctgcgttgaa
     1921 ggtcgtcagt cccctgaagt catcctcgag ctgcaccccg aagcgcagat agttcggtcc
     1981 ccaggaattg cgacgtgccg aaaaatccag tccgtagtgg ccgaacgggt cgtgatccag
     2041 ctgatagtcc agcatttcga gattgccgcg accgtagagt gctttgacct gctgctccag
     2101 cgcggccgga tcgagcgtct gaccgacgaa ttgtccgaac aagtcatcaa tctggcgctg
     2161 ataggttttg gagtcgggct gcaccttcac gtagtcgatt ctcggcaaac catcgcgtac
     2221 cgagcggcgc gccagcagat agcgttgata ctcctcagcg gaaacctcga gcgccgacaa
     2281 ccgcggctct gccgaccaga ccgcggtctc tccggcattg acgatcttca tcgtgtccgg
     2341 aaagtcgtag gaggagaaat ccccgagctg cagcgtgacg acgatgtcct tcggcgagag
     2401 catggccaga tcgcgctcga tgttccggcg aagcaaaatt gccagcatct gattggtgat
     2461 gctcggcagc gaattgagat tcttagcagg ctgcagtgga aagccggcgt caacgacgat
     2521 cagcacatcg acacccatcg ctcgcgcaac gtcgatcggc aggttgtcgg ccagcccgcc
     2581 atccaccagg atttggttgt gatcttcgac cggcgcgaac acacccggga ccgacatact
     2641 ggcgcgcatc gcagtggtca gatcgccact gcccagcacg actttggcac cggtctcaag
     2701 attggtggcc accgcgcgaa acggcgtcgg cagctggtcg aagtccgtga ttcgggccac
     2761 gggcagcgtc atcttgcgca gcgtttccgt caggagctga ccctgaagca atcccttcgg
     2821 gatgaccagg cgtctgccct gcagtcccag cggtagattc accaggaact ggcgatcctc
     2881 ctccttgcgc cggtagtcca gctgtgtgcg cggcgggcgg tcgcggaacg cggcctgcca
     2941 gttgagggcg gccatcgcgt gctcgatctc cacgccggtc atcccactgg cgtacaaacc
     3001 accgaccacg gcccccatgc tggtgcccgc gatcacatca accggcacat gcagacgatc
     3061 cagcatcttg aggacgccaa tgtgcgcggc accgcgcgcg ccaccgccag acagcaccag
     3121 accaatgcgg ggtctgccag ggttggccgc tgcgggaggg actgccgact cgctggcagg
     3181 ttcctgtgcg cccgcgctca cggatagcaa cagcaacgcc gcgagggcaa cggtcttggc
     3241 taaatgcatg cccagtggtg gcctgtgatg gctgcgcgcg caaatcttag catgcacccc
     3301 atacatcgct ggcgccgcat acctgctacc accccaaccg tcctgccatt aagctggcgc
     3361 aatgggctca tacctaggtc tttccatcgc gtcgatcatc atccagatcg ggctcatcgt
     3421 gcattgcatc cgcaccggtc ggagtttcct gtgggtctgg gtactgctgg ccctgcccgg
     3481 ggtcggctcg atcgcctatg tcctggtgga gatcctgccg gggctgctcg gcagccgcgg
     3541 cgcacgtcgg gccgcgcgcg gcgtcagtcg cgcactcaac cccgagcagg atctgcgccg
     3601 atacgaagcc gaggcgcgtc acaccggcga tgtcgccagc cgacagcgct acgcggatga
     3661 gctgctgcgg cacgatcggg cgggcgatgc ggtcgaggtc tacaagcaga cgctcaaggg
     3721 gctgtacgag cacgatccga acctgatgct cggtctggcg cgcgcgcaat ttgccaacgg
     3781 cgcattcgcc gaagcgcgct ccacgcttga tgcgttgatt gagcacaacc ccgatttcaa
     3841 atcccccgac gggcacctgc tgtacgcccg cgcgctcgag ggcgaaggca atcgcgacaa
     3901 ggcgcttgag gaatacgctg cggttgccgg ctactacgcc ggcgccgaag ccaaactgcg
     3961 ctatgcacgc ctgctcagtt cgctgggaaa aaaagaagac tcgcgccgga tactgaaaga
     4021 gctgctggag cacgcgcgct tcgccccgcg ccactaccgc aagatgcagc agcagtggct
     4081 cgccgatgcg gaacgcgagc tggcatcact ctagaatggt gcccgacgtt tctgtcggcg
     4141 cggctgctgc tgcgtaatgc agtgcaaatc gccaccgccc agcagcacct cgcgcgcggc
     4201 cactccgacg atccgacggc gcggaaagat ccggcgcagc tgctggcgca cctgcgcatc
     4261 ggtgcgcgca tccagcaacg gcaggatgac ggcgttgttg gcgaggtaga aattggcata
     4321 gctcgcggcc aggcgcgtgc cggcgcgcag cggccggata ctatcgtccg aaacgatgcc
     4381 ggcggcctcg cgccgtgtca tgtacagcgg ccccggtgag accagcttgt ggatccgcaa
     4441 ccgccggcca cgcgcgtcgc gtgccgccat cagacgctcg aacgcatcca tcgacacgcg
     4501 gtgctgtggg tcacgcggat catcgcacca ggtcaggcac acctcaccgg gtcgcgcgaa
     4561 acaggcgaga ttgtcgatat ggcccgaggt ttcgtcattg atcacgccct cgcccagcca
     4621 gatcacgcac gtcacaccca ggtagcgtct cagcagcgtc tcgatctgcg cgcgcttgag
     4681 gccgggattg cggttgcgat tgagcagaca ctgctccgtc acgagcacgg tgccctggcc
     4741 gtcgacatga atcgccccgc cttcgttgac gaacggcgcg tgatagcgtt cgaggccggt
     4801 cagctccagc actttctgcg cgacctggtt gtcgtgttca gaatcgttgt agatccctcc
     4861 ccacgcattg aagcgccagt gcacgccgcg catcgctccg gcggcattga ccacgaagct
     4921 ggggccggtg tcacgcatcc aggcatcatc gctggagagc tctaccaggc gcacggagtc
     4981 tgccggcatc gccgcacggg cgcgggcgaa ctgccgtgcc gaggcgccaa tggtgaccgg
     5041 ttcaaactgg gagatggcag tggcgaccgc ggcgaacgcc tgctgcgccg gctgggcgtt
     5101 gcgccgccag ttatcgggtc gctcaggcca cagcatccag gtgccctcgt gcggctcgaa
     5161 ctccgccggc atccagtagc catcgcgcag cggattggac ctcagggtgc gtgacatgat
     5221 gcgcgccatt ctatcaacga tgcaccggcg tgaattcccg caacagctcg ctgtgataac
     5281 atttcagatt ctcggttact ccgtgagaat ccagtcatga ccaatcatcc gagctctgcc
     5341 aaaccgatcg tgccctccgc gggtcagaag atcaccctga aagacggcaa gctgtcggtt
     5401 ccggatcgtc cggtcgtgcc gttcatcgaa ggcgatggca cgggccgcga tatctggcgc
     5461 gccagcgttc gggtactcga cgctgcggtc aagaaggctt acggtgctcg ccggcaaatc
     5521 gagtggatgg aagtctacgc cggtgagaag gcgttcaatg cgttcaagag ctggctgccc
     5581 gacgaaaccg tgcaagcctg ccgcgactac ctggtgtcga tcaagggtcc gctgaccaca
     5641 ccggtcggcg gtggaatccg ctcgctgaac gtggcgctgc gccagttgct ggatctctat
     5701 gtctgcctgc gcccggtgcg ctggttcaag ggcgtaccct cgccggtcaa ggcaccggaa
     5761 aaggtggaca tggtgatctt tcgcgagaac accgaagatc tgtattccgg catcgagttc
     5821 gaagagggca ccgaggacaa ccgcaaattc ctcgagctgc tgaagaccaa cttccccaaa
     5881 cactacggca agatccgatt tcccaatacc agcggcatcg gcatcaagaa catttcgatc
     5941 gaaggcaccg agcgtatctt ccgggcggcg atcgagtatg cgctgcgcaa ccggcgcaag
     6001 agcctcacga tcgtacacaa gggcaacatc ctgaagttca ccgaaggctc gtttcgcagc
     6061 tggtcgtacg cgctggccga gcgcgagttc ggcgatcgcg tctatacctg gccgcagtgg
     6121 gagcgcacca aggcagcttc cggtgagaag gccgcgaacg acgaacaggc ggcagcgctc
     6181 aaggccggca agctgctcat caaggacgcg atcgctgaca tcacgctgca acaggtactg
     6241 acgcgggccg acgaattcga cgtggtcgcc accatgaatc tcaacggcga ctatctgtcg
     6301 gatgcgctgg cagctcaggt gggcggcatc ggcatcgctc cgggtgccaa catcaactat
     6361 ctgaccgggc acgcagtatt cgaggccacg cacggcacgg cgcccaagta cgccgatctc
     6421 gaccaggtca atcccggctc agtgatcctg tccggcgaga tgatgctgcg atacatggga
     6481 tggaccgaag ctgccgacgc ggtgatcagc gcgatggaca agaccatcgg tcagaagatt
     6541 gtcacatacg acttcgcgcg tctgatggaa ggcgcgacga aagtgaagtg tagcgaattc
     6601 ggcgatgcgc tgattcgaaa tctctagcgc agcgcgcgca ctcagacggg aaaacccccg
     6661 cctagaactt cctctgatcc ggcggcggcg gcgtgtattg atagatccac gtctctgtga
     6721 gcggctggcc attgactgcc agatacagcc gcagattgat cggctcggtg ctgtcatccg
     6781 cgggtttgag atcgaacatc gcgcgccagc cgtgaatcga atgcagcgga cgcgccgagg
     6841 tgatttcgac ctgaccgcgc gaagccgtga tcaccggctc gacgcgcgca tcctcgccga
     6901 gcagcgggat atcaccgccg gcgaagtcga cgacgaaacg ccacgaaaag taggtgtgct
     6961 tctgcccgac gatgccgccg atgccatccc gcgtcgccgc cacctgtgcc aggggatagg
     7021 acaccggatt gtatttgcac cagtacaagc gataggcaaa gagccactcc tggccgcgct
     7081 ggggcttgtc ggccggcgtc cagaaggcca ccacattgtc gaaggtctca tcgaccgtcg
     7141 gaatctcgac cagcatgacg gcgcctttgc cccagttgcc gcgcggctcc acccagcaac
     7201 tgggtcgtcg gtcgtagaac acaccgtcat cctgatagtg atcgaaatcg cgatcacgct
     7261 gcatcagtcc gaaaccgtgc ggggagtcgt caaggaacga attgacgcgc accaccgcag
     7321 ggttcaccag cggacgccac aaccattcgt tcgcgccgtt gttgatctgc agtccgtcgg
     7381 aatcgtgtac ctgcggccgc cagtcagcgc ttgcccgacg atcgttcgag ccatagaaaa
     7441 acatgctggt acccggcgcg attcccagcc tctcgatcgt cttgcgcgga tagagcgccg
     7501 catcgatatc ggtcacgatc gaatcggaca catcgatgcc aaaacggtaa gcgccggtga
     7561 cgctcggtga atccagcaga gcgtagacgg tcagacggct cgagccgttg gcgggtcgct
     7621 ccagatagta ggcgatgaaa tcagggaact cctcgggttt cgtcgagccg cagtcgatgg
     7681 ccagtcctcg ctgcgacatg ccgtactgtt tgtcgctgtc cactgcgcgg aaataggagg
     7741 cgccctggaa ggcggcgaca tcacgcaccc agtcggtgtg aaacagcagg cggaagccgg
     7801 cgaaaccgag attcgccggc agtttgcgtc cgtcgacgcc gctgcgactg tagtcgaaca
     7861 tggcgctgtc gtacgccagt tcctgcgcct ggcctttgtc gaccgtgtac atgcgcaccg
     7921 gcttggtaac ggtgaatcca agatgaaaga agcgcacctg aaaccgcagg ccctcgccga
     7981 accacaatga atgatcatcg cggtagcgaa tggactgcca ttgatcccag ctcagcttgg
     8041 aaatcggtcc gggcaggggt gcgttgttgg gtttgtatgc cgcggcggcc atcgcgcgcg
     8101 ccagcccctt caggcgcgcg tagtcaaagg cttccgactg tcccaaacgc ttgagcggcg
     8161 agggatgcgc tggctccgcg gcgggtgtct cgctcaacga caatccaccc gtgatcaccg
     8221 cggcaccggc ctgcaagaaa cgacgtctgg acatatctga ctggctccag cggactgagt
     8281 gtcgcgacgc ggcgacaaga tcattgtact tcgcattggc gcagaattgc cgccggcgac
     8341 agatcgcgca gcctggctga atatttttca aggtgctggc gctcgcccgc ctgtgggaca
     8401 ttcggccgac aggtacgtca tcgcgctgac ccacggcgag gagtgcttca ggaatactgt
     8461 ggcgcgccgt tgggtccgga agtctgtcaa agctttaccc gctgggattg ctat
//
LOCUS       DATMRM010000011         9109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4343185, whole genome shotgun sequence.
ACCESSION   DATMRM010000011 DATMRM010000000
VERSION     DATMRM010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9109
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4343185"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2115)
                     /locus_tag="VNZ06_00340"
     CDS             complement(<1..2115)
                     /locus_tag="VNZ06_00340"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWW19224.1"
                     /translation="MNELRAALNQYVAGTLPRGVTEQRVADAAAKDPQMVPAMLALIE
                     TYRSAGGLNSEFARALQNVLRSSSAAPPPSVPPGPASSATTPPGATAAKLSLGSVLKE
                     RFALEAIVSGGDQGGMGVVYRARDLVKQEAMDRHPYVAIKVLNESFKQHPDSMKALQR
                     EARRAQTLSHPNIINVHDFDRDGGNVFLVMELLDGNALDVLIRRYRPKGGMPIAEALY
                     IIEGLGRALAHAHARGIVHSDFKPGNAFLTSDNIVKVLDFGIARATKLGGGDKTLFDA
                     GKLGALSPSYACPEQFEHLGPDPRDDVYALAVVTYELLTGRHPFEKTDPETPHEIIKV
                     DSVSARSSGLKPAPIPGLSRRQWRTMKSALAFSRLQRPRDAGAFLEGMLPRKVPVRNV
                     LFASAAGLLVLVLSAQLLSNYIERVRLQRLTQKLESSDPQVLSVALQKLQSFPAEERA
                     AALVNPLVERNVIGYFTRRARDEFDPSAGKYNYRDAIAGLKGVQDLSPMYADSRQLND
                     TIDKVETARKSEILHQAEQFDSQLRHGLLIANQGPNNAQAALAALAQLDPNHPYLSSK
                     RLPIALAEQTRASLLAGNLAQAAAFLDVGLAIDSGNIQLRDLQDQVHRRQEQLQQLAL
                     IASQERVLIPLSKAGSTLEDFRAQRAALNQMQRSSPDNRVLAEAQNRLTSLVGVSVSD
                     DLKKGRAADAQSLLGEFGYLLSE"
     gene            complement(2260..4215)
                     /locus_tag="VNZ06_00345"
     CDS             complement(2260..4215)
                     /locus_tag="VNZ06_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWW19225.1"
                     /translation="MTTKDSQWPSIALSLVLLLMTAPSRSAPLEAYGRLPKIEDIILS
                     PDGSRIAYVQTLANDHRVIAVRSLADQKVVGGLDAGNVKLRSLGWADNSHLMVVTSAT
                     DLPERNFLFKREWYRLLIYDLDSKREEVLPDPARLGGQWNSRSNVLRIIQGRIWVRHP
                     NGGTAVTLVTDIYENGEWRPMLVRVDLATYAETILDRGSNDSRDWLIDADGQVVGAAL
                     YRESQKRWSLLFRRDGRMTEIASQQTDTDPPIVLGFGPEPDTLLMSRLEDGDPVWRTL
                     SLKDGSFSAARVGRETLDAPIEDYKTYRMVGGVHVDDDARYIFFDPTMQARWDAVVRS
                     FPGEHVRLTSYTADFSKLVVLVNGSKDGLTYQLIDLATMKRQLLAHQYDDVGAPNEVR
                     RITYSASDGLLVPAYVTLPSDKPPKNLPLIVMAHGGPAARDSADFDWWSQALASQGYA
                     VLRANYRGSTLGWLHYSAGFGEWGGRMQTDLSDGVRYLAAQGMIDPARVCIVGASYGG
                     YAALAGVSLQSGIYRCAVSISGQSDLKRFLHWIDDARQGGNQLEQRTLERFTGATSES
                     DPKLDAISPARHADAIQVPVLLIHGQDDTVVPFEQSQEMYDAMRRAHKDVQLVKLKHE
                     DHWLSRGDTRWQMLQVSTAFLLERNPP"
     gene            complement(4445..5233)
                     /locus_tag="VNZ06_00350"
     CDS             complement(4445..5233)
                     /locus_tag="VNZ06_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWW19226.1"
                     /translation="MKIVAVTNIKGGVGKTTTAVNLAYLCAAGGRHTLLWDLDPQGAA
                     TYTLRCEPTTNASAKKLVSGKLDLPEVLIPSGYRNLDVLPSDFSYRNFDVHLSARRHP
                     TERLLKMSRSLREVYAALCLDCPPGISLLSENVLRAADALIVPLLPTPLSVRMLAQLR
                     DFIAANEWNDLVLLPFFSMVDRRKALHHEVMAGTREQFPQILKTVVPYWSEIERMSLR
                     RAPLPSYSPHSEAGQIYAALWKEVQERMHWKVTAAQQATIDQKA"
     gene            5382..7142
                     /locus_tag="VNZ06_00355"
     CDS             5382..7142
                     /locus_tag="VNZ06_00355"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWW19227.1"
                     /translation="MSSSFDLLIRNGRIVDGTGAEPFHGSIAVRDGAISAVGQVEGTA
                     RREIDAEGLLVTPGFVDIHTHYDGQVTWGHALTPSSVHGVTTAVMGNCGVGFAPCRPA
                     DRERLVRLMEGVEDIPHPVLAQGLPWNWETFPDYLDRLSERQYDIDFAAQLPHGALRV
                     YVMGERGANREPASPDDVSRMAQLAQQAVSAGALGFTTSRTMNHRTSDGEPTPTLTAE
                     QDELVGIAMALGEIGEGVLQVVSDFRDRINELRIFRTMVQQSGRPLSISLTQHNRSPQ
                     GWRAILEFISRANADGLPVRAQVAGRPVGVLFGLELTHNPFSAHPSYRAIADRPFAQR
                     LQRLRDPAFQARLLAEQPELECDPFMKRMYGNFDNLYLLDERPDYEPSPSASLGARAR
                     ERGSSVLRLALDIMTAGDGRAMLYYPVLNYAEHSLEPSLEMMRHPHTILGLGDGGAHC
                     GTICDASFTTHMLTHWARDRSRGERLPLPWVVKAHAQSTAAAVGLMDRGVLATGYKAD
                     INLIDFQRLSLRRPEVRYDLPAGGRRLMQTADGYVATIVSGEVVYQNGEATGELPGRL
                     VRGRQARPAQSAQIGVSGHD"
     gene            7135..8160
                     /locus_tag="VNZ06_00360"
     CDS             7135..8160
                     /locus_tag="VNZ06_00360"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HWW19228.1"
                     /translation="MTDQPRVLEPHAAWLRDDLADPSLWTEQFTDEELRELERAMRHA
                     QTVSNDLLQLSKQDFPLPAVAPRLQRIENELINGRGFALLRGLSRAQYSNDELCLLYW
                     GIGLHLGNPWPQNQHGHMLGDVTDQGKKRDDYGVRGNELPGPLPYHSDGSDLVGLLCL
                     QMAAEGGLSAICNALAIHNDMVRQHPQLAAELYQPQPNDFRGEQAPGKTSWYLMPIFT
                     DFENRLFVRYIRTYIKASQLLPEAPRVSVRAEQAMQLLDRMTEDPKYHITMDLRPGDM
                     QFINNYHVLHARTDYRDDRASGRVRHLKRLWLETTVLKQRPRYFARKDRDHWLTNKSI
                     SRLDRTG"
     gene            8157..8723
                     /locus_tag="VNZ06_00365"
     CDS             8157..8723
                     /locus_tag="VNZ06_00365"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004704714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HWW19229.1"
                     /translation="MKAKRRCAWAEGNALHEAYHDAEWGVPVRDSRELWELLMLEGFQ
                     AGLAWIVVLKKREALRTAFRQFDPEKVARFTDRDVQRLLKDEGIIRSRAKINATIDGA
                     RIWLKMRDAGEDFATWMWQFVGGKPLQRNGPVAAQTPLSVEISKALKKRGFRFVGPVI
                     VYAFMQAAGIVNDHAPHCFRRSAVAKLR"
     gene            complement(8732..>9109)
                     /locus_tag="VNZ06_00370"
     CDS             complement(8732..>9109)
                     /locus_tag="VNZ06_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19230.1"
                     /translation="KFAVRGFSESVRHELAMSHSPVRLSVVHPGGVKTNIARNSQAGA
                     YLRDRADRAPLLQRFEQMARTTPAAAASRILRGIARNEPRILVGRDARVMDWIQRLRP
                     ARYWSIMARTVLRRAGGQPPASS"
BASE COUNT         1729 a         2884 c         2841 g         1655 t
ORIGIN      
        1 ctcggaaagc agatagccga attcaccgag taacgattgc gcgtcggccg ctcggccctt
       61 cttcaaatcg tcgctgacgc tgactccgac caaagaggtc agccggttct gcgcctcggc
      121 gagtacgcgg ttgtcaggcg agctgcgctg catctgattg agcgctgcgc gctgcgcgcg
      181 aaaatcctcc agcgtcgatc cagccttgct cagcggaatc agtacgcgtt cctgtgatgc
      241 gatcagcgct agctgttgca gctgctcctg gcggcgatgc acctggtcct gcagatcgcg
      301 tagctgaatg ttgcccgaat cgatcgccag gcccacatcg agaaacgccg cagcctgcgc
      361 caggttaccc gccaacaggc tggctcgtgt ctgctcagcc agtgcgatcg gcaggcgctt
      421 gctgctcaaa tagggatggt tcggatcgag ctgcgccagc gcggcgaggg cggcctgggc
      481 attgttcggt ccctggttgg cgatcagcag cccgtgtcgt agctgcgaat cgaactgttc
      541 ggcctgatgc aggatctcgg atttgcgcgc agtttccacc ttgtcgatcg tgtcgttcag
      601 ttgacgcgaa tcggcgtaca tcggactgag gtcctgtaca cctttcagac cggcgatcgc
      661 atcccggtaa ttgtacttgc ccgcagacgg gtcgaactca tcgcgcgcgc gccgcgtgaa
      721 gtagccgatc acattgcgtt ccaccagcgg attcaccagt gcggcggcgc gctcttcggc
      781 cggaaaactc tgcagcttct gcagcgccac ggacagtacc tgcgggtcgg aggattccag
      841 tttctgcgtg agtcgctgca gcctgacacg ctcgatgtag tttgatagca gctgcgccga
      901 tagcaccagc accagcaacc cggcggcgct ggcaaacagc acattcctga ccggaacctt
      961 gcgcggcagc atcccctcga gaaaggcgcc ggcatcacgg ggtcgctgca gacgcgagaa
     1021 ggcaagagca ctcttcatcg tgcgccactg tcggcgcgac agacccggaa tcggcgctgg
     1081 cttcagcccg gaactgcgcg ccgatacaga atccaccttg atgatctcat ggggtgtctc
     1141 cggatcggtt ttctcgaatg gatgacggcc ggtcagcagc tcataggtga ccacggccag
     1201 cgcgtaaacg tcatcacgcg gatcggggcc aagatgctcg aactgctcgg ggcatgcgta
     1261 ggagggagac agcgcgccga gcttgccggc gtcgaacaac gtcttgtcac cgccgccaag
     1321 cttggtggcc cgcgcgatgc caaagtccag aactttgacg atgttgtccg aggtgaggaa
     1381 cgcgttaccg ggcttgaagt ccgaatgcac gatgccgcgc gcatgagcat gcgccagagc
     1441 tctgcctaaa ccttcgatga tgtacagggc ttctgcgatc ggcatgccgc ccttggggcg
     1501 atagcggcgg atcagcacgt ccagggcgtt gccgtcgagc agctccatga ccaggaacac
     1561 gttgccacca tcgcgatcga agtcatgaac gttgatgatg ttcggatgtg acagcgtctg
     1621 cgcgcggcgc gcttcacgct gcagcgcctt catcgaatcc ggatgctgct tgaagctctc
     1681 gttcagcacc ttgatcgcaa cgtagggatg tcgatccatc gcctcctgct tgaccagatc
     1741 ccgcgcgcgg tagaccacgc ccatgccgcc ctgatcaccg ccgctgacga tggcttcgag
     1801 agcaaagcgt tccttgagta cgctgccgag cgagagcttg gccgcggtcg cgccgggtgg
     1861 cgtggtggcc gaactcgccg gcccgggtgg aaccgacggt ggcggcgcag ccgaggaact
     1921 gcgcagcaca ttttgcagtg cacgcgcgaa ctcggaattc aggccgccgg cactgcgata
     1981 ggtttcgatc agcgccagca tcgccggcac catctgtgga tccttcgcgg cggcatcagc
     2041 gacgcgctgc tccgtcacgc cacggggcaa ggttccagca acgtactgat tgagggcagc
     2101 gcgtagttcg ttcatgttat ggcgcagcgc agcagcaagc ggactcctcc gtccctgcgc
     2161 ggagtcgaaa ccacagccaa aagtctacac ggtgcagccc gagttgggat gcgttcacgc
     2221 gcggaaattt gcgcgttatg tcgcgagcgc cgcccctcat caagggggat tgcgttccaa
     2281 tagaaaggcg gtcgacacct gcagcatctg ccatcgggta tcgcctcgcg agagccaatg
     2341 atcctcgtgt ttcagcttga ccagctgcac gtccttgtgc gcccgtcgca tcgcatcgta
     2401 catctcctgg ctctgctcga aggggacgac cgtgtcgtcc tgaccatgaa tgagcagcac
     2461 cggcacctgg atcgcatcgg cgtgccgagc cggtgaaatc gcatcgagtt tgggatccga
     2521 ctcactggta gcgccggtga accgctcgag cgtgcgctgc tccaactgat tgccgccttg
     2581 ccgcgcatcg tcgatccagt gcagaaagcg cttcagatca gactgtccgg agattgagac
     2641 cgcacagcga tagatgcccg attgcaggct gacccctgcc agcgccgcgt agccaccata
     2701 gctggcgcca acgatgcata cccgcgccgg atcgatcatt ccctgggccg cgaggtagcg
     2761 tacgccatcc gacagatcgg tctgcattct cccgccccat tcaccgaaac cggcggaata
     2821 gtgcaaccag ccaagggtcg agccgcgata gttcgcgcgc agcaccgcat acccctgtga
     2881 ggccagcgcc tgggaccacc agtcgaaatc cgcgctgtcg cgagccgcgg gtccgccgtg
     2941 cgccatgacg atcagcggaa gattcttggg cggcttgtcg gaaggcagcg tcacataggc
     3001 ggggaccaac aagccatcac tcgcgctata ggtgatgcgc cgcacttcgt tgggagctcc
     3061 cacatcatcg tattggtgcg caagcagctg gcgcttcatc gtcgccagat cgatcagctg
     3121 gtaggtcaac ccatctttgg atccgttgac cagcacgacc agtttggaga agtcggccgt
     3181 gtacgaggtc aggcgcacgt gttcacctgg aaatgaacgc acaacggcgt cccagcgcgc
     3241 ctgcatggtc gggtcaaaga aaatatagcg cgcgtcatca tccacatgga cgccgcctac
     3301 catgcgatag gtcttgtagt cctcgatcgg agcgtccagc gtctcgcgac cgaccctcgc
     3361 ggcactgaaa gagccatcct tcagcgacag cgtgcgccag accggatcgc catcctccag
     3421 gcgggacatg agcagcgtgt ccggctcggg cccgaatcca agaacgatcg gaggatcggt
     3481 atcagtctgc tgcgaagcga tctccgtcat ccgaccgtcg cggcgaaaca gcaacgacca
     3541 acgcttctga ctttccctgt agagcgctgc tccaacgacc tggccatcgg cgtcgatcag
     3601 ccagtcgcgc gaatcgttcg aacctctatc cagaatcgtc tcggcgtacg tcgccagatc
     3661 aacgcgcacc agcatcgggc gccactcgcc gttctcatag atatccgtca cgagcgtcac
     3721 cgccgtaccg ccgttgggat gtcgcaccca gatgcggccc tggatgatcc tgagcacatt
     3781 ggagcggcta ttccattgcc cgcccaaccg tgccggatcc ggcagcacct cttcgcgctt
     3841 gctgtccagg tcgtagatga gtagccgata ccattcgcgc ttgaacagga agttccgctc
     3901 cggcaaatcg gtcgcggagg tgacgaccat cagatgtgaa ttgtccgccc agcccaggga
     3961 ccgcagcttg acattgccgg cgtccagccc gccgacaact ttctggtctg ccagagatcg
     4021 caccgcgatc acgcgatggt cattggccag tgtctgaacg taagcgatgc gcgagccgtc
     4081 gggtgacagg atgatgtctt caatcttggg aaggcgcccg tacgcctcca atggcgcgct
     4141 tcgactcggc gctgtcatca acagcagcac gaggctcagc gcgatgcttg gccattgaga
     4201 gtcttttgtt gtcatcgggt ccttccctgc gaggccgcca tgaaccgcat tattcctgcg
     4261 ttttggccac cgtccgtcat ggtgccgaca gaaggggtgc cccgcctgcc ccccgaaggg
     4321 acggggtcgt ctggagcgta tcagtttttg agctggcgtc gcaccttcaa cagcagatcg
     4381 tgcggcgtag gctcctcccc gcagcctgcg tcgcgaggac tacgcgccgt ggcgggtgcg
     4441 caaatcaggc tttctggtct atcgtcgcct gctgtgcggc agtcactttc cagtgcatgc
     4501 gctcctggac ttccttccac agtgccgcat agatctggcc agcttcgctg tgcggtgaat
     4561 aggacggtaa cggagcgcgc cgcagactca tgcgctcgat ttcgctccag tacggcacca
     4621 cggtcttcag aatctgcgga aactgttcgc gcgtgccggc catcacttca tggtgcaggg
     4681 ctttgcgccg gtccaccatc gagaagaacg gtaacagcac cagatcgttc cattcattcg
     4741 ccgcgatgaa atcacgcagt tgagccagca tgcggaccga caacggcgtt ggcagtagcg
     4801 gcacgatcag ggcgtccgcc gcgcgcagca cattctccga caacagcgaa ataccgggcg
     4861 ggcagtccag gcacagcgca gcgtagacct cgcgcagcga ccgactcatc ttcagcagac
     4921 gctcggtcgg gtgcctgcgt gcactcagat gcacgtcgaa attgcgatag gaaaaatctg
     4981 acggcagcac gtcgagattg cgatatccgc tcggaatcag cacttccggc agatcgagct
     5041 tgccgctgac caactttttg gccgaagcgt tggtcgtcgg ctcacagcgc aaggtatagg
     5101 ttgcggcgcc ctgcggatcc agatcccaca gcaaggtgtg acgcccaccg gctgcgcaca
     5161 ggtaagcgag gttgaccgcg gtcgtcgtct tgccaacgcc gcccttgatg ttcgtgacgg
     5221 caacaatctt catattctcg caggcatact ggcacaagtt tccgagcggc accaaatggc
     5281 tcacgttatg caatggtcgg caaaccatct tgacccccgg gtgcggcagg gctgaaaatt
     5341 aagtgaaagc aaagatatcg agcgcttacc aggggtaaag catgagcagc tctttcgatc
     5401 tcctcatccg caacggtcgc atcgttgacg gcaccggcgc ggaacccttt catggcagca
     5461 tcgcggtgcg tgatggcgcg atcagtgccg tcggccaggt cgaaggcacc gcacggcgcg
     5521 agatcgacgc cgagggactg ctggtcactc cgggctttgt cgacatccac acgcactatg
     5581 atggacaggt cacgtggggc cacgcgctga cgccctcctc ggtgcacggt gtcacgacgg
     5641 cggtgatggg taactgtggt gtgggctttg cgccctgccg cccggccgat cgcgagcgcc
     5701 tcgttcgctt gatggagggc gtggaagaca ttccgcatcc ggtgctggcg caaggcctgc
     5761 cgtggaactg ggaaaccttt cccgactatc tcgatcgatt gtccgagcgg caatacgaca
     5821 tcgacttcgc tgcgcaactg ccgcacggcg cgctgcgcgt ctatgtgatg ggggagcgcg
     5881 gtgccaaccg tgagcccgcc agccctgacg atgtctcacg catggcacag ctggcgcagc
     5941 aggccgtgtc ggccggtgcc ctcggtttca ccacgtcgcg cactatgaat caccgcacca
     6001 gcgacggcga accgacaccg acgctgaccg cggagcagga tgaactggtt ggaatcgcga
     6061 tggccctcgg agagattggc gaaggcgtac tgcaggtcgt gtcggacttt cgcgaccgga
     6121 tcaacgagct gcgcatcttt cgaacgatgg tgcagcagtc tggtcgcccg ctgtcgatct
     6181 cattgacgca gcacaatcgc tcgccgcagg gctggcgagc aattttggag ttcatcagca
     6241 gggccaatgc ggacggattg ccggtgcgcg cacaagtggc cgggcggccc gtgggcgtat
     6301 tgttcggact cgagctcact cacaacccgt tctccgccca tccgagttac cgcgctatcg
     6361 ccgaccgtcc cttcgcgcag cgactgcagc ggctgcgcga tccggcgttt caggcgcggc
     6421 tgctggcgga gcagcccgaa ctcgaatgcg atccgttcat gaagcgcatg tacggcaact
     6481 tcgacaacct gtacctgctc gatgagcgcc cggactatga accgagcccg agcgccagtc
     6541 tgggcgcgcg tgcacgcgag cgcggcagca gcgtgctcag gctggctctg gatatcatga
     6601 cggccgggga tggccgggcc atgctgtact acccggttct caactacgcc gagcactcgc
     6661 tcgagccgtc actggagatg atgcgtcatc cccacaccat tctgggattg ggcgacggtg
     6721 gggcgcattg cggcacgatc tgcgatgcca gcttcaccac gcacatgctc acgcactggg
     6781 cacgcgatcg tagccgcggc gaacgactgc cgctgccctg ggtcgtcaag gcccatgccc
     6841 agagcacggc cgcggcggtg ggtctcatgg accgcggcgt gttggcgacc ggatacaagg
     6901 ccgacatcaa cctgatcgac ttccaacgcc tgagtctgcg gcgccccgaa gtgcgctatg
     6961 acctgccggc gggcggccgg cgcctgatgc agacagcaga cggctatgtc gccacaatcg
     7021 tcagcggcga ggtggtctat cagaacggag aggccacagg ggagttgccg ggtcggctgg
     7081 tgcgcggccg tcaggcacga ccggcccaga gcgcgcaaat aggggtgagc ggccatgact
     7141 gatcagccac gggttctgga accgcatgcc gcgtggctgc gtgatgacct cgccgatcct
     7201 tcactgtgga ccgagcaatt cacggatgag gagctgcgcg aactggaacg agcgatgcgg
     7261 cacgcgcaga cggtcagtaa tgatcttctg cagctctcga agcaggactt tccgctaccc
     7321 gcggtggcgc cgcgcctgca gagaatcgag aacgagttga tcaacggccg cggcttcgcg
     7381 ctgctgcgcg gactgtcacg cgcgcaatac agcaacgatg agctgtgtct gctctactgg
     7441 ggcatcggcc tgcatctggg caacccttgg ccacagaacc agcacggtca catgctgggc
     7501 gacgtcaccg atcaaggcaa gaagcgcgat gactacggcg tgcgcggcaa cgaactgccc
     7561 gggcccctgc cctatcatag cgatggctcg gatctggtgg gcctgctgtg tttgcagatg
     7621 gcggctgagg gcggcctgtc ggcgatctgc aatgccctgg cgattcacaa cgacatggtg
     7681 cgtcagcatc cgcagctcgc cgccgaactg tatcagccgc agccgaacga ttttcgcggc
     7741 gaacaggcac ctggcaagac cagctggtat ctgatgccga tcttcaccga cttcgagaac
     7801 cggctgttcg tgcgctacat ccgcacttac atcaaggcct cgcaactgct gcccgaggcg
     7861 ccgcgcgtca gcgtccgcgc cgagcaagcc atgcagctgc tggatcgcat gaccgaggac
     7921 ccgaagtacc acatcacgat ggatctgcga cccggcgaca tgcaattcat caacaactac
     7981 cacgtgctgc atgcgcggac cgactatcgc gacgatcgcg ccagcggccg cgtccgtcac
     8041 ctcaagcgtc tgtggctcga gaccacggtc ctgaagcaac gccccaggta cttcgcgcgc
     8101 aaggaccgcg accactggct caccaacaag agcatcagcc ggttggatcg caccggatga
     8161 aggccaagcg gcgctgcgcg tgggccgagg gcaacgcgct gcatgaggcc tatcatgacg
     8221 ctgaatgggg cgtacccgtg cgcgatagcc gtgagctttg ggaactgctg atgctggaag
     8281 gctttcaagc gggcctggcg tggatcgtgg tgctaaagaa gcgcgaggcg ctgcgcacgg
     8341 ctttcaggca gttcgatccc gagaaggtcg cccggttcac ggaccgcgac gtgcagcgac
     8401 tactgaagga cgaaggcatc atccgctcgc gcgccaagat caatgcgacc attgacgggg
     8461 cgcgtatctg gctgaagatg cgcgatgccg gtgaggactt cgcgacctgg atgtggcaat
     8521 tcgtgggcgg caagcccctt cagcgcaacg ggccggtggc agcacagacg cctctgtcag
     8581 tcgagatctc gaaggctctc aagaaaaggg gcttcaggtt cgtcggcccg gtgatcgtct
     8641 acgcattcat gcaggcagcg ggcatcgtga acgatcacgc gccgcattgc ttccggcgca
     8701 gtgcggtcgc aaagctgcgc tgaagggacg actagctcga agccggcggc tggccaccag
     8761 ccctgcgcag gacggttcgg gccatgatcg accagtaacg agccggccgc aggcgctgga
     8821 tccagtccat gactcgcgcg tccctgccga ccaaaattct gggctcgttg cgcgcgatgc
     8881 cgcgaaggat gcgcgacgcc gcagccgccg gtgtggtgcg tgccatctgc tcgaaacgct
     8941 gcagcagcgg tgcgcgatcg gcccggtcgc gcagatacgc gcccgcctga gagttgcggg
     9001 cgatgttcgt cttgacgcct cccggatgca ccaccgacag acgtaccggg ctgtggctca
     9061 tcgccagttc atgacgtacg ctctcggaga agccacggac cgcgaactt
//
LOCUS       DATMRM010000012        11938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4033370, whole genome shotgun sequence.
ACCESSION   DATMRM010000012 DATMRM010000000
VERSION     DATMRM010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11938
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4033370"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1918)
                     /gene="mdoH"
                     /locus_tag="VNZ06_00375"
     CDS             complement(<1..1918)
                     /gene="mdoH"
                     /locus_tag="VNZ06_00375"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003962.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HWW19231.1"
                     /translation="MDTMKQSTDNDRWLQHQALWRRSLFFGLTFASALAGGFLMLDIL
                     KANGVSALELLSLLMFFVLFTWISGAFWTAIAGFFVRLIGRDPAQLQLNEVASHVLQS
                     RTAIVIPIYNEDAARVFAGVEAIWGSLQQQPHAQAQCFDFFILSDTRKPEIAREEERC
                     WHALSARAAAAERIYYRRRADNIGRKSGNIAEFVRSWGAAYDHMIVLDADSIMSGSAL
                     VSLTQLMETHPDVGVIQALPLLTGRDTLFARILQFAVRVSGPMFASGIAFWQLGESNY
                     WGHNAILRMKPFADYCALPKLPGKPPFGGEIMSHDIVEAAFIRRAGYKVWLVANIPGS
                     WEEIPSNLIDFAARDRRWAQGNLQHVGVMPMHGLHWLSRIHLLTGILSYVSSPLWLLV
                     LIVTSIVTCAEAVTGHHYFQAGAHTLFPSWPQYRDGEIAALLIMTVVVLFVPKMLGAY
                     LTLRDKSARAAFGGSRKFLLSCMIEQALSMLMAPIMMLFHSEFVLRALFGRSVGWDAQ
                     PRGDRGVTWHEAFLRHRWHVAIGLVWGTVIVMLAPDFIWWMLPVVVGMLIAVPFTTFT
                     SRSDLGLALRRHGLLLTPEETSPPPELVAAERARERAHAPLALLEPQPINAPDTVAIN
                     LVMLPPRAPLPMIAS"
     gene            complement(1906..3450)
                     /locus_tag="VNZ06_00380"
     CDS             complement(1906..3450)
                     /locus_tag="VNZ06_00380"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HWW19232.1"
                     /translation="MRTRFWLLLTVTAIAVVIEHSSAVSPQHKAALPAPAPRFGFESV
                     QHLAQQRSEEAYRDRSSKLPDSIAKLSYEDYRTIQFRPDNALWRNQAMFEVQFFHRGS
                     SFDRRVNVTEVGEDGELLPVNYSPAQFDYGKVSVPKDLPADLGFAGLRVHYPLQRPDY
                     KDELIAFLGASYFRMLGRDQSYGASARGLAINVASTNGGEEYPYFTDFWLVRPSPEQR
                     TLTVYALLDSPSLTGAYHFEIVPGNTTTLEVTATLYPRKAVDKLGLAPMTSMFFYGED
                     RPRPYDDYRPEVHDSDGLMMQTGAGEWLWRVLSNPKALRISSFSDEHPRGFGLSQRDR
                     DFAHYQDEDARYQQRPSYWVAPLGDWGKGTVELVEIPTDESIHDNIVAFWVPKSPLQP
                     HKPFSFGYLLSAYSQASNWPPGGRAVATRTAPAHGRDNSRRLLIDFSGGDLDALVGNQ
                     PVKAALGAHNGDIDNVTVQRLAENGMWRVSLRLQPKNTQGVDLHCYLTLYGEALTETW
                     MYLWTP"
     gene            3717..5327
                     /locus_tag="VNZ06_00385"
     CDS             3717..5327
                     /locus_tag="VNZ06_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWW19233.1"
                     /translation="MGNLLQTKVRPGDPQFEANASMMQGLVVQLRERTQALAQGGSLS
                     ARERHRARGKLLVRDRIELLLDPGAPFLELSPLAAYDMYGGEVPAAGMVTGIGRVSGT
                     QCVIVANDATVKGGTYYPLTVKKHLRAQEIAAENQLPCIYLVDSGGAFLPMQDEVFPD
                     REHFGRIFFNQATLSARGVPQLACVMGSCTAGGAYVPAMSDESVIVKDNGTIFLGGPP
                     LVRAATGEVVSAEELGGADVHTRISGVADHYAQSDEHALSILRRIVANLNRRSPSTPA
                     LQAVEEPRYDPAELYGIIPADSRKPYDVREVIARVVDGSRLDEFRQRYGNTLVTGFAH
                     LYGCPVAILANNGILFSESALKGAHFIELASQRGIPLLFLQNISGFIVGKKYENAGIA
                     KDGAKLVTAVATSAVPKLTLLIGGSFGAGNYGMCGRAYGPRFLWSWPNARISVMGGEQ
                     AASVLATVRRESLEAQGKSWSAQDEEAFKAPTRAQFEAQGTAYYATARLWDDGIIDPV
                     QTRRVLGLSLFAALNAPIGQTRFGVFRM"
     gene            5334..6125
                     /locus_tag="VNZ06_00390"
     CDS             5334..6125
                     /locus_tag="VNZ06_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWW19234.1"
                     /translation="MADFETLQLESDRGCTTVWLNRPDRHNAFHEGVIADLTRCFERL
                     STDPAVRMIVLAGRGRSFCAGADLEWMQRQASAHYDTNIADARRLAIMLRTIAQTPKP
                     ILARVHGAALGGGLGLICVCDLAIAAPEATFGTTEVRLGLTPSTIAPYVLNAIGERAA
                     GRYFLTGERFDAHEALRIGLVHELVEADALDAQLAKMLDALRQSGPKAQAHCKELVAL
                     LRDRDPTEPLILEQTARSIADVRSGEEAREGIAAFLAKRKPGWIG"
     gene            6131..8128
                     /locus_tag="VNZ06_00395"
     CDS             6131..8128
                     /locus_tag="VNZ06_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HWW19235.1"
                     /translation="MFHRILIANRGEIACRIIATCRRLGVETVAVYSEADANARHVRL
                     ADQAIGVGPAAARDSYLRMDRIIEAAQRSGAQAIHPGYGFLSENPEFALRVAEAGLTF
                     IGPTVEAMRAMSSKATAKQLMERSGVPLLPGYHGDDQDASVLRREAERIGYPVLIKAT
                     AGGGGKGMRVVENAGQFDERLASCQREAKGSFGDARVLLEKFLLRPRHIEVQIFGDTH
                     GQVVHLFERDCSSQRRHQKVLEEALAPHLGPEQRESVTRAACSAARAVGYVGAGTVEF
                     LFDPGVGFYFMEMNTRIQVEHPVTEMITGLDLVEWQLRVAVGEPLPKSQADISSSGHA
                     IEVRLYAEVPESGFLPSSGTIKLFDLPVRAPGVRIESGIEAGDAVGIDYDPMLAKLIV
                     HADSRPEAVALLLRALSEVRIGGVGNNVAFLRRLAASPEFARGEVDIGFIERTPSIVH
                     GAQLAPEPRALAAAALWVLTHETWMARGDADGVWARNDGWRLNAHLIRTLQFETGTAQ
                     PALATVFIHYAPGAVRVAVGDPAAAVPASLQAEGEGLFHLLFGAEGARVRVAPDGEYL
                     RLGVGDAEYRLRWRDPRQPVVEGYLADSGLAAPMPGRIIAHVVAAGNTVSRGAPLLIM
                     EAMKMEHTICAPANGVVRAYLAAVGQQVREGTELIDFEAQQ"
     gene            8154..9218
                     /locus_tag="VNZ06_00400"
     CDS             8154..9218
                     /locus_tag="VNZ06_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWW19236.1"
                     /translation="MEPSAAIWARRLMLSALLLGLGLLCLLILWPFVTPIVWAAILAF
                     ASWPLYRKLRHRMHHWHTIPALVMTLLLGCAVIVPVLWIMVLVQRELLLAYQSLSKYL
                     AEGPHTLPAFLTNIPWLGEQLQEYTNRYQADPTEITQDVSAYTLSWARNIGGILSSVG
                     RNVARLSLTLITLFFFYLDGAAIAHQTHRVIQRFFGDRIDRYIEIAGRMTRAVVYGLL
                     ITSLLQGVIAAIGYWIAGLKAPVLLGFLTGILSVVPMVGTAIVWVPAGIWLLSSGHVG
                     WGVFLLIWSAALVYPADNVLRPILISSSVSVPFLLVMFGALGGLQAFGLMGLFLGPVL
                     LATGLALWREWSEQPAAPVP"
     gene            9255..10151
                     /gene="prpB"
                     /locus_tag="VNZ06_00405"
     CDS             9255..10151
                     /gene="prpB"
                     /locus_tag="VNZ06_00405"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016142557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HWW19237.1"
                     /translation="MPSETTSSAGSRLRAAVAEERPLQVVGAINAYVARMAEATGFRA
                     IYLSGGGLAANSLGVPDLGISALEDVLIDIRRISDASGLPLLVDADTGWGGAFNIART
                     VRSMIKAGAAGMHIEDQVSAKRCGHRPGKEIVPLEEMVDRVKAAVDARTDHGFVIMAR
                     TDALASEGLERAIERARACVDAGADMIFPEAVRDLTQYRKFKDALAVPILANITEFGH
                     TPLYTRDELGSVGVDIVLYCCSAYRAMNAAALATYQAIRRDGTQKHVLEQMQTRADLY
                     KYLDYDAFEQRLDRLFAAGKGK"
     gene            10152..11279
                     /gene="prpC"
                     /locus_tag="VNZ06_00410"
     CDS             10152..11279
                     /gene="prpC"
                     /locus_tag="VNZ06_00410"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HWW19238.1"
                     /translation="MAKKSVALSGVVAGNTALCTVGLTGNDLHYRGYDILDIADICEF
                     EEIAYLLIHEKFPNTRELAAYKARLRKARDLPPALRQALELLPPDSHPMDVLRSGVSV
                     LGCLEPEGLKHESDGARDIADRLIACLSPMLCYWYHYSHSGKRIEINTDDDSIAGHFL
                     HLLHGKPPSALWTRAMHTSLNLYAEHEFNASTFTARVIAGTGSDLYSCITGAIGALRG
                     PKHGGANEIAFQIQERYDSPDRAEADITQRVSNKEVIIGFGHPVYTTGDPRNEVIKRV
                     ARRLAEAAHDLRHYAIAERIESVMWSAKKMFPNLDWFSAVSYHLMSVPTAMFTPLFVV
                     ARTTGWAAHVIEQRIDGKIIRPSANYTGPENLKFVALKDRP"
     gene            11276..>11938
                     /locus_tag="VNZ06_00415"
     CDS             11276..>11938
                     /locus_tag="VNZ06_00415"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWW19239.1"
                     /translation="MSSHDYKSAQRPAPDAVLTAIADYAIHYRQPSDLARETAYYCLM
                     DTLGCGFQALRYPACTRMMGPVVPGAQMRGGARVPGTGYELDPVAAAFNIGTMIRWLD
                     FNDTWLAAEWGHPSDNLGGILAVADYLSRAASVQGRAVIPVSTLLRAMIQAHEIQGVL
                     ALENSFNRVGLDHVLLVRVASTAVVSALLGGTREQVINAVSNAWIDGGALRTYRHAPN
                     TGS"
BASE COUNT         2170 a         3650 c         3920 g         2198 t
ORIGIN      
        1 cactggcgat catcggcagc ggcgcccgcg gcggcaacat caccaggttg atcgccaccg
       61 tgtccggcgc attgatcggt tgcggctcga gcagcgcaag gggggcatgg gcccgctcgc
      121 gtgcccgctc cgcggctacc agttctggcg gcggcgaggt ctcttcgggg gtcagcaaca
      181 gaccgtggcg gcgcagcgca agccctaagt ctgatcggct ggtaaaggtg gtgaagggga
      241 ccgcgatcag catgccaacc acgaccggca gcatccacca gatgaaatcc ggcgccagca
      301 tcacaatcac ggtgccccaa accaggccga tggccacgtg ccaacgatgg cgcagaaagg
      361 cttcgtgcca agtgacgcca cgatcgcccc gaggctgagc gtcccagccg acgctgcggc
      421 caaacagcgc gcgcaagacg aactcggagt gaaacagcat catgatggga gccatcaaca
      481 tgctcagtgc ctgctcgatc atgcagctca gcaggaactt gcgtgagcca ccaaacgccg
      541 cgcgcgcgct cttgtcgcgc agcgtcagat aagccccgag catcttcggc acgaacagca
      601 cgaccaccgt catgatcagc agtgcggcga tctcgccatc gcgatactgc ggccagctcg
      661 ggaatagcgt gtgcgcgccg gcctggaaat agtggtgacc ggtgacggcc tccgcgcagg
      721 tgacgatcga ggtcacgatc agcaccagca gccacagcgg tgagctgaca taggacagga
      781 tgccggtcag cagatggatg cggctcagcc agtgcaggcc atgcatcggc atgacgccga
      841 catgctgtag gttgccctgc gcccagcgcc ggtcgcgcgc tgcgaagtcg atcaggttgg
      901 aggggatttc ctcccagctt cccgggatgt tcgccaccag ccacaccttg taaccggcgc
      961 gacggatgaa cgcagcctcg acgatgtcgt ggctcatgat ctcgccgccg aacggcggtt
     1021 tgccgggcag ttttggcagt gcgcagtagt cagcgaacgg cttcatgcgc aggatcgcgt
     1081 tgtgacccca gtagttgctc tcgccgagct gccagaaggc gataccgctg gcgaacatcg
     1141 gtccgctcac gcgtaccgcg aattgcagga tgcgcgcgaa caacgtgtcg cggccggtca
     1201 gcagcggcag ggcctgaatc acgccgacgt ccggatgcgt ttccatgagc tgcgtcagtg
     1261 agaccagcgc tgacccggac atgatgctgt cggcgtccag aacgatcatg tgatcgtagg
     1321 cggcgcccca gctgcgcacg aattcagcaa tattgccgga cttgcgaccg atgttatcgg
     1381 cgcgccgccg gtagtagata cgctccgcgg ccgctgcacg cgcgctgagc gcgtgccagc
     1441 agcgctcctc ttcccgggcg atctcgggct tgcgggtatc ggacaggatg aagaaatcaa
     1501 aacactgagc ctgcgcatgc ggctgctgtt gcagcgatcc ccagatggct tcgacaccgg
     1561 caaacacgcg cgcagcatct tcgttgtaga tcggaatcac gatcgcggtc cggctctgca
     1621 ggacgtggga tgcgacctcg ttgagttgta gctgcgcagg gtcgcggcca atcagccgta
     1681 cgaaaaagcc ggcgatcgcg gtccagaaag cgcctgagat ccaggtgaac agcacgaaga
     1741 acatcaatag cgacagcagc tcgagcgcgg acacgccatt ggccttcaga atgtcgagca
     1801 tcaggaagcc accggccagc gccgaggcga aagtgagtcc gaagaacaat gagcgacgcc
     1861 agagcgcctg atgctgcagc cagcggtcgt tgtcagtgct ctgcttcatg gtgtccatag
     1921 atacatccag gtctcggtca gggcttcccc gtacagagtc agatagcaat gcagatcgac
     1981 accctgggta ttcttcggct gcagtcgaag acttacccgc cacataccat tttccgccag
     2041 tcgctgcacg gtcacgttgt ctatgtcgcc attgtgcgcc ccgagggcgg ctttcaccgg
     2101 ttggttgccg accaaagcat ccaggtctcc acccgagaag tcgatcagca ggcgccgact
     2161 attgtcgcga ccgtgcgccg gtgccgtgcg tgtcgcgacc gcccgtccgc ccggcggcca
     2221 attggaggcc tgactatagg cggacaacaa gtaaccgaag ctgaacggct tatgcggctg
     2281 cagtgggctc ttgggtaccc agaaggcgac gatgttgtcg tgaatggact cgtcggttgg
     2341 gatttcaacc aattccacgg tgcccttgcc ccaatccccg agtggcgcga cccagtagct
     2401 gggacgttgc tggtagcgcg catcctcatc ctggtagtgc gcaaagtcgc ggtcgcgctg
     2461 gctcaggccg aacccccgcg ggtgctcatc cgaaaagctg gagattcgca gcgccttggg
     2521 attgctcagc acgcgccaaa gccactcccc cgcgccggtc tgcatcatca gcccgtcgct
     2581 gtcatgcact tcaggacggt agtcgtcgta agggcggggc cggtcctctc cgtaaaagaa
     2641 catcgaggtc atcggcgcaa gtcccagttt gtccaccgcc ttgcgcggat acagggtggc
     2701 ggtgacttcg agcgtcgtgg tgttacctgg aacgatttca aagtgatacg cgccggtgag
     2761 gctcggtgag tccagcagcg catagaccgt cagggtgcgc tgctcgggtg acggccggac
     2821 cagccagaaa tcggtaaagt aggggtactc ctcgccaccg ttggtgctcg cgacgttgat
     2881 cgcaagtccg cgggcggagg caccgtagct ctggtctcga cccagcatcc tgaaatacga
     2941 ggcccccaga aaggcgatca gctcatcctt gtaatcgggc cgctgcaacg gatagtgaac
     3001 ccgcagtccg gcaaacccca ggtccgccgg caggtccttg ggtaccgaga ccttgccgta
     3061 gtcgaactgc gccgggctgt agttcacagg gagaagctcg ccgtcttcac ctacctccgt
     3121 gacgttgacc cggcgatcga agctcgagcc ccggtgaaag aactggactt cgaacatcgc
     3181 ctgattgcgc cagagcgcgt tgtccggccg gaactgaatg gtgcgatagt cctcgtagct
     3241 gagcttggcg atgctgtccg gaagcttgct gctacgatct cggtaggctt cctcggagcg
     3301 ctgctgcgcc agatgctgca cgctctcaaa cccgaaacga ggggccggcg ccggcagggc
     3361 ggccttgtgc tggggtgata cggcgctgga atgttcgatc acgactgcga tcgccgtcac
     3421 tgtcagcagc aaccagaatc gcgtccgcac tgcgtactcc tagcgtcatc aacgacaaag
     3481 gcctccaacg ctgcaccgga tcgccctcgc tcacggcgct tactgttaaa caggggccgt
     3541 cgagatattg gcattgccag aggcggcgct gagcgttgaa gtctctgaaa gccttgtcgg
     3601 acttgtgagg acagagcata catggcggca atgaatccga agctgaatac taagaagtga
     3661 acggcggttc actttttgga ttggctaggg tacagtccgc tcgcgacctt cagagcatgg
     3721 gcaatctcct ccagacaaaa gtccgacctg gcgatccgca gttcgaggcc aatgccagca
     3781 tgatgcaggg gctggtggtg caactgcgtg agcgcacgca agccttggcg cagggcggca
     3841 gcctgagtgc gcgcgagcgt catcgcgccc gcggcaaact gctggtgcgc gatcggatcg
     3901 agctgctgct cgatccgggt gcgccgtttc tcgagctcag cccgctggcg gcgtatgaca
     3961 tgtacggcgg ggaggtgccg gcggccggga tggtgaccgg tatcggccgc gtcagtggca
     4021 cccaatgtgt gatcgtcgcc aacgatgcca ccgtcaaagg cggtacttac tacccgctga
     4081 ccgtcaagaa gcatctgcgc gcgcaggaga ttgcagccga gaatcagctg ccctgcatct
     4141 atctggtgga ctctggtgga gctttcctgc cgatgcagga tgaggtgttc cctgacaggg
     4201 agcatttcgg acgcattttc ttcaaccagg cgacgctgtc ggcgcgaggc gtgccgcagt
     4261 tggcctgtgt gatgggctcc tgcaccgccg gtggcgcgta tgtgccggcg atgtccgatg
     4321 agagcgtcat cgtcaaagac aacggcacga tcttcctggg cgggccgccg ctggtcagag
     4381 cggccaccgg cgaggtcgtg agcgcggagg agctgggtgg tgccgacgtg cacacgcgca
     4441 tctccggcgt ggccgatcac tatgcgcaaa gcgatgaaca cgcactgtcg atcctgcgcc
     4501 gcatcgtcgc caatctgaac cggcgttcac cgtcaacgcc ggcactgcaa gcggtggagg
     4561 aaccgcgcta cgacccggct gagctctatg gaatcatccc ggccgacagc cgcaagccct
     4621 atgatgtacg cgaagtgatc gcgcgcgtgg tggacggttc gcgtctcgat gaattccgac
     4681 agcgctatgg caacacattg gtcactggat tcgcgcacct ctatggctgt ccggtcgcca
     4741 ttctggccaa caacggcatt ctcttctcag agtcggcgct caagggtgct cacttcatcg
     4801 agctggcctc gcaacgcggt atcccgctgc tgttcctgca gaacatctcc ggattcatcg
     4861 tcggcaagaa gtatgaaaac gccggcatcg ccaaagacgg cgccaagctg gtcacggcgg
     4921 tcgcgaccag tgcggtaccc aagctgacac tgctgatcgg tggctcgttc ggagcaggga
     4981 actacggcat gtgcgggcgg gcgtacggcc cgcgctttct atggagctgg ccgaacgcgc
     5041 gaatttcggt gatgggcggc gagcaggccg ccagtgtact ggcgaccgtg cgccgagagt
     5101 cgctggaggc ccagggcaag agctggagcg cgcaggatga ggaggccttc aaggcgccca
     5161 cccgggcgca gttcgaggcc cagggcaccg cgtactatgc gactgcgcgc ctgtgggacg
     5221 atggcatcat cgatccggtg cagacacggc gcgtgctggg attgagcctg tttgccgcac
     5281 tcaatgcacc catcggtcag acgcgcttcg gcgtgttccg gatgtgaggc ctcatggccg
     5341 actttgaaac gttgcaactc gagtccgacc gcggatgcac gacggtgtgg ctcaatcgtc
     5401 cggatcggca caacgccttt cacgaaggcg tgatcgcgga cctgacgcgc tgtttcgagc
     5461 gcctgtcaac ggatccggcg gtgcgcatga tcgtgcttgc cggccgcggg cgcagtttct
     5521 gcgccggcgc ggatctggaa tggatgcagc gccaggcatc ggctcactac gatacgaaca
     5581 tcgccgatgc gcggcgcctg gcaatcatgc tgcggacgat cgcgcagaca ccgaaaccca
     5641 tcctggcacg cgtgcatggg gccgcgctcg gtggcgggct cggattgatc tgcgtgtgcg
     5701 acctggcgat tgcggcaccc gaggccacgt ttgggacgac tgaagtgcga ctcgggctga
     5761 caccctccac catcgctccc tatgtcctca atgccatcgg cgagcgcgcg gccgggcgct
     5821 acttcctgac gggtgagcgc ttcgatgcgc atgaggcgct gcgcatcggg ctggttcacg
     5881 aactggttga agccgatgcg ctcgatgcgc aactggcgaa gatgctggat gcgctgcgtc
     5941 agtcgggccc gaaggcacag gcgcactgca aggagctggt cgctctgctg cgtgatcgcg
     6001 atccgaccga gccactgatt ctggaacaga ccgcgcgcag catcgccgac gtgcgttcgg
     6061 gtgaggaagc gcgcgaaggc atcgcggcgt tcctcgccaa gcgaaaaccc ggctggatcg
     6121 gttaacgggt gtgtttcatc gcattctcat cgccaatcgc ggcgaaatcg cctgccgcat
     6181 catcgcaacc tgccgtcggc tcggcgtcga gacggtggcg gtctattccg aggcggatgc
     6241 gaatgcccgc cacgtgcggc tggccgatca ggcgatcggt gtcggcccgg cagccgcgcg
     6301 cgacagttat ctgcggatgg atcggatcat cgaggccgcg cagcgcagcg gcgcacaggc
     6361 gatccatccg ggctatgggt ttctatccga gaatccggag ttcgcactgc gcgtcgccga
     6421 ggcgggcctg accttcatcg gtccgacggt ggaagcgatg cgcgccatga gctcgaaggc
     6481 cacggccaag cagctcatgg agcgctcagg cgtacccctg ctgcccggct accacggtga
     6541 cgatcaggat gcatcggtgc tgcgtcgtga agccgagcgc atcggctatc cggtgctcat
     6601 caaggccacc gccggcggcg gcggcaaggg catgcgcgtg gtcgagaacg cggggcagtt
     6661 cgacgaacgg ctcgccagct gccagcgcga agccaagggc agtttcggcg acgcgcgggt
     6721 actgctggaa aaatttctgc tgcgcccgcg acacatcgaa gtgcagatct tcggcgatac
     6781 acatgggcag gtcgtgcacc tgttcgagcg cgactgctct tcgcagcgac ggcatcagaa
     6841 ggtgctggaa gaagcgctgg cgccgcacct ggggcccgag cagcgcgaga gcgtgacacg
     6901 cgcggcctgc agtgcggctc gcgcggtggg ctatgtcggt gctggcacag tcgagtttct
     6961 gttcgacccg ggtgtgggat tctatttcat ggaaatgaat acccgcattc aggtcgaaca
     7021 tccggtgacc gagatgatca cgggtctgga cctggtggaa tggcagttgc gcgtcgccgt
     7081 gggcgagccc ttgccgaagt cgcaggcgga catctcgtcg agcgggcacg cgattgaagt
     7141 gcgactgtac gccgaagtac cggagtcggg cttcctgccc tcgagcggta cgatcaagct
     7201 gtttgatctg ccggtacgag cgccgggcgt tcggattgag agtggtatcg aagcggggga
     7261 cgctgtcggt atcgactatg acccgatgct ggcgaaactc attgtgcatg ccgactcgcg
     7321 gcctgaggcc gtggcgctgc tgctgcgcgc gctgtcggaa gtgcgcatcg gcggagtcgg
     7381 caacaatgtt gcgttcctgc gtcggctggc cgcgagcccc gagttcgcac gcggtgaggt
     7441 ggatatcggt ttcatcgagc gcaccccgtc gatcgtgcac ggcgcgcaac tcgcgcctga
     7501 accgcgtgcg ctggcggccg ccgccctgtg ggtgctgacg catgagacct ggatggcgcg
     7561 aggtgacgcg gacggcgtct gggctcgcaa cgatggctgg cgactcaatg cgcatctgat
     7621 ccgtacgctg cagttcgaga ctggcacagc gcagccagcg ctggcaacgg tgttcattca
     7681 ctacgcaccg ggcgcggtgc gggtggcggt gggcgatccg gccgcagccg tcccggcatc
     7741 cctccaggct gaaggcgagg gcctgtttca tctgctgttc ggcgccgagg gcgcgcgcgt
     7801 acgggtggcg ccggacggcg agtatttgcg cctcggagta ggcgatgcgg agtaccgact
     7861 gcggtggcgc gatccgcgcc agccggtggt ggaggggtac ctggccgata gcggcctcgc
     7921 ggcaccgatg cccgggcgta tcattgcgca tgtggtcgcc gccggaaaca ccgtgagcag
     7981 aggcgcgccg ctgctgatca tggaagcgat gaagatggag cacacgatct gtgcgcccgc
     8041 caacggcgtc gtgcgcgcct atctcgcggc cgtcggtcag caggtgaggg aaggtactga
     8101 actgatcgat ttcgaggcgc agcaatgaaa ttgccgaccc ggctgacata gcgatggaac
     8161 cgagtgctgc catctgggcc cggcgcctga tgctctcggc gctgttgctg ggattgggtc
     8221 tgctgtgtct gctgattctc tggcccttcg tcacgccgat cgtctgggcg gcgatcctgg
     8281 cgttcgccag ttggccgctg tatcgcaagc tgcgtcatcg gatgcaccac tggcacacga
     8341 tccctgcctt agtgatgacg ctgttgctcg gctgcgccgt cattgtgccg gtgctgtgga
     8401 tcatggtgct ggtccagcgc gagctgctgc tggcctatca aagcttgagc aagtacctgg
     8461 ccgaggggcc gcatacattg cccgcattcc tgaccaacat tccctggctg ggtgagcaac
     8521 tgcaggaata caccaaccgc tatcaggccg atcccaccga aattacgcag gatgtgagcg
     8581 cctatacctt gagctgggcc agaaatatcg gcggcatcct cagttcggtc ggccgcaatg
     8641 tcgcgcggct ttccctgacg ctgatcacgc tgttcttttt ctatctcgat ggcgccgcca
     8701 tcgcgcatca gacccatcgg gtgatacagc ggttctttgg cgatcgcatc gatcgctaca
     8761 tcgagatcgc gggtcgcatg acgcgcgcgg tcgtgtacgg gctattgatc accagtctgt
     8821 tgcagggtgt gatcgcggcg atcggctact ggatagcggg tttgaaggca ccggttctgc
     8881 tcggctttct caccggtatc ctgtcggtgg tgccaatggt gggaaccgcc atcgtatggg
     8941 tacccgccgg tatatggtta ttgtcaagcg gacatgtagg gtggggcgtg ttccttctga
     9001 tctggagcgc ggcgctcgtg tacccggcag acaatgtgct gcgtcctatc ctcatcagca
     9061 gctcggtcag cgtccccttc ctactcgtca tgtttggcgc gctgggcggt ctgcaggcct
     9121 ttggactcat gggtctgttt ctcggaccgg tgctactggc aaccggtctg gctctgtggc
     9181 gtgaatggag cgagcaaccg gcggcgccag ttccataaat cctgtccact agtgtagggt
     9241 tcagccccgt gaaaatacct tccgaaacca cttcctccgc tggttcccga ttgcgcgccg
     9301 cggtggccga agagcgacca ctacaagtcg tcggggcgat caacgcctac gttgcgcgca
     9361 tggcggaagc gaccggattt cgcgcgatct atctgtccgg tggcggcctg gctgccaact
     9421 cgctgggcgt acccgatctg ggtatcagcg cgctggagga tgtgctgatc gacattcgtc
     9481 gcatcagcga tgcctcgggt ctgccattgc tggtcgatgc ggataccggc tggggcggcg
     9541 cgttcaacat cgcgcgcacg gtacgctcga tgatcaaagc cggcgcggcg gggatgcata
     9601 tcgaggatca ggtctcggcc aagcgctgcg gccatcgacc cggcaaggaa atcgtgccgc
     9661 tggaagaaat ggtcgatcgc gtcaaggcgg cggttgatgc gcgtacggat cacggcttcg
     9721 tgatcatggc gcgcaccgat gcgctggcga gcgaagggct ggagcgcgca atcgagcgcg
     9781 cacgcgcatg cgtggatgcc ggggccgaca tgatttttcc ggaagcggtt cgcgatctga
     9841 cgcagtaccg caaattcaag gatgcgctcg cagtgccgat cctggccaac atcaccgagt
     9901 tcggtcacac cccgctgtac acgcgcgatg agctaggctc ggtcggtgtg gatatcgtgc
     9961 tgtattgttg ttctgcgtat cgtgcgatga acgctgcagc gctggcgacc taccaggcga
    10021 tccgccgcga cggcacgcaa aagcacgtgc tcgagcagat gcagacccgt gcggatctgt
    10081 acaagtatct ggactacgat gccttcgaac aaaggctgga tcgactgttc gcggctggaa
    10141 aggggaagta ggtggcgaag aaatccgtcg ctctgtcggg agtggtcgcc ggcaataccg
    10201 cgctgtgcac ggtgggactg acgggcaacg acctgcacta ccgcggctat gacatcctgg
    10261 atatcgccga catctgcgag ttcgaggaaa tcgcatacct tctgattcat gaaaagtttc
    10321 cgaacacgcg cgagctggct gcctacaagg cacggctcag gaaggcgcgc gatctgccgc
    10381 cggcgctgcg ccaggcgctg gagctgctgc cgcctgacag ccatccgatg gatgtgctgc
    10441 ggagcggcgt ttcggtgctc ggatgtctgg aaccggaggg actcaagcac gagagcgacg
    10501 gtgcgcggga catcgccgac cggctgattg cctgcctgtc cccgatgctg tgctactggt
    10561 accactacag ccacagtggc aagcgcatcg agatcaatac cgacgacgat tcgatcgccg
    10621 gccactttct gcatctgctg cacggcaagc cgcccagtgc gttgtggacc cgtgcgatgc
    10681 atacctcgct caatctttat gcggaacacg agttcaacgc atcgactttc accgcgcgtg
    10741 tgatcgcggg caccggctcg gatctgtatt cctgcatcac tggcgcgatc ggcgcgttgc
    10801 gcggacccaa gcacggcgga gccaacgaga tcgcgttcca gattcaggag cgctacgact
    10861 cgcccgatag ggcagaagcg gacatcaccc agcgcgtctc gaacaaggaa gtcatcatcg
    10921 gcttcggtca tccggtctac accaccggcg atccgcgcaa tgaagtcatc aagcgcgttg
    10981 ccaggcgcct ggccgaagcc gcgcacgacc tgcgccatta tgcgattgcc gaacgcatcg
    11041 aatcggtgat gtggtcggcc aagaagatgt ttcccaatct cgactggttc tccgcggtgt
    11101 cctatcacct gatgagtgtg ccgacggcga tgttcacgcc gctgttcgtc gtggcacgca
    11161 ccaccggctg ggcggcgcac gtcatcgagc agcgcatcga cggcaagatc atccgcccga
    11221 gtgcgaatta caccgggccc gagaatctca agttcgtcgc gctcaaggac cgaccatgag
    11281 ctcacacgac tacaaatcgg cgcagcgtcc tgcgccggac gcggtactca ccgccatcgc
    11341 ggactacgcg atccactatc gccagcccag tgatctggct cgggagaccg cctactactg
    11401 cctgatggac acgctcggtt gcggctttca ggcactgcgc tacccggcct gcacgcgcat
    11461 gatgggcccg gtggtcccgg gtgcgcagat gcgcggcgga gcgcgcgtgc caggcaccgg
    11521 ttacgagctc gacccggtcg ccgcggcgtt caacatcggg acaatgattc gctggctgga
    11581 tttcaacgac acctggctgg cggccgaatg gggccatccg tcggacaatc tcggcggcat
    11641 cctcgcggtc gcggactatc tgtcgcgtgc cgcctcggtg cagggccgcg ccgtcattcc
    11701 ggtcagcact ctgcttcggg cgatgatcca ggcgcatgag attcaaggcg tgctcgcact
    11761 cgagaatagt ttcaaccggg ttggtctgga tcatgtgctg ctggtgcggg tggcctcgac
    11821 cgcagtggtg agcgcgctgc tgggaggcac gcgcgagcag gtcatcaatg cggtgtcgaa
    11881 tgcctggatc gacggcggcg cgctgcgcac ctaccggcac gcccccaaca ccggctcg
//
LOCUS       DATMRM010000013         6647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2174717, whole genome shotgun sequence.
ACCESSION   DATMRM010000013 DATMRM010000000
VERSION     DATMRM010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174717"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VNZ06_00420"
     CDS             <1..472
                     /locus_tag="VNZ06_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWW19240.1"
                     /translation="EFFSSAYGLRGARQRERIEWAIAQFELGDQLRGTSAQLPGGYKQ
                     RLAMAAALLHEPEILFLDEPTSGADPLARRAFWARITSLAQQGVTVIVTTHFMEEAEY
                     CDRIAIMDAGRILAQGSPAQVRALGHAASEQPTMDDAFVSIVTRARRDRAAQPA"
     gene            469..1611
                     /locus_tag="VNZ06_00425"
     CDS             469..1611
                     /locus_tag="VNZ06_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWW19241.1"
                     /translation="MSPSVRPYWRIKLQRVRALIIKESRQIVRDPSSIAIGVILPLIL
                     ILLFGYGLSLDVRNVPIAVVMDQPSTQGQELLAALQLSPYFNPRQVATMPAAENLMAH
                     GEVDGIVHLAQEFDRRMSAGDAQLQLIVQGGDANRARIIEAYVQGAVGVWAAHRAAQG
                     RGGSVGPVLVQSRLWFNDANESHYFLVPGLIVLVMTLIGGLLTAMVVAREWERGTYEA
                     LLVTPVRSEEILLGKTLPYFVLALIGFSLCALSAQFLFHVPLRGSFWILLFDSMLYVL
                     VALGIGLLISSWVKSQLVASQLTMLVTFMPAYILSGFLFDLRSMPLAVRFVTYLLPAR
                     YYVALLQSVFLAGDVWSVIVPNTLVLALMAGLLALASRAVMRKRLE"
     gene            1617..2735
                     /locus_tag="VNZ06_00430"
     CDS             1617..2735
                     /locus_tag="VNZ06_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWW19242.1"
                     /translation="MLASIYRILALTRKELLAVLKDRRGRMSLLIPPVVQALIFGYAA
                     TYDLNHVPYAVFDQSHSGASQQLLAHIDGSGVFQRVATLSQAQDIAREVDSRKALLAL
                     VIDSDFERRMFSGQGAQLQVIADGRNSNTAGTAMGYLGAVVKRFNTQWRRDHGLGPPS
                     LTITARAWYNPNLETRWYMIPGMIGTLTLLQTLMLTAMSVAREREQGTFDQLLVTPFR
                     PYELMAGKALPSMLVGIIQATGVLLVAQLWFRIPFAGSYLTLYIGLLVFLMAAVGMGL
                     LVSAASATMQQAMLYSMLLIMPFALLSGLTTPVSNMPGWLQYLTAINPLRYAIDITRR
                     VYLEGAGLALLGGDLLPLAIVAAATLSISSWVFAHRMQ"
     gene            2735..4234
                     /locus_tag="VNZ06_00435"
     CDS             2735..4234
                     /locus_tag="VNZ06_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWW19243.1"
                     /translation="MKRGVRRPIGWLRWLCCGLIASGCSVGPNFVRPQARTPEHWSAA
                     SAPAAAPRASHLSEDGQLAERWWLQFNDTGMQSLIERALKANLDLRIAMQRVEEARAQ
                     RDVTGAQAWPRLSVGASYTRARLSQTTPTGSLFSSVGQISLPGGAHVNVPLSYNQFQT
                     DADASWEIDLFGRVRRALEAANAQIQVSIEDEHAVQLTLMSDVAQSYIELRGAQLRKA
                     TAVENIATLSELLELTQQRRDAGLTSDEDVSRSAAQLDATRSDLPALELQIVEGINAL
                     SALLAREPDALRAELETAAPIPAVPESVAIGVPADLARRRPDVREAEASLHVATAQIG
                     VATADLFPRLVLSAQGGLQSQALSQLGEWASRFGSLGPSFQLPIFDRGQWRTVHLDKL
                     RAQESALAYQRTVIQALREVANALAAFNADQDRRVQLDRAVSESRDALALARERYQSG
                     VTNFIDVLDAQRTLQQNESALIDAISATSVDLVALYRALGGGWSDAMDH"
     gene            4218..5330
                     /locus_tag="VNZ06_00440"
     CDS             4218..5330
                     /locus_tag="VNZ06_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HWW19244.1"
                     /translation="MRWITRIALALLLVVGVALLSLALVVSHNGACVSAPGPSADAER
                     MKAIEYRCYGTADVLKVETVVRPVPADNQVRVQVRAASINPLDWHYLHGTPYLMRLSA
                     GIGTPKDPGLGADFAGVVESVGRSVTHFKAGDAVFGQADGTLAEFVTLPEDGSLASKP
                     DNISFEQAAAIPVAGVTALQALRDQAHVLPGQKVLINGASGGVGTFAVQIAKALGAEV
                     TGVCSTHNVEMVRSLGADHVVDYTKEDFTKSDQRYDVVLDNVVNHSLWDYRHVLTPKG
                     ILVIVGGGSSNENPWIGAFQAPLKASVQSLFVSPRWVMFFADVSAKNLTAMHDLVASG
                     KVTPVIDRHYALDETATAMRYLEQGHARGKIVIDIP"
     gene            complement(5414..5785)
                     /locus_tag="VNZ06_00445"
     CDS             complement(5414..5785)
                     /locus_tag="VNZ06_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWW19245.1"
                     /translation="MLLLDTSFLIEFEREIAANQPGVATAVLAMHRSMPVAISVVALG
                     EFAEGFTDPQALNEFVSHFRVLQLSRAIAWRTAPLQTSLSRRLGENDAWIAATALCYG
                     ATLIGRERAFERVPRLDYLEF"
     gene            complement(5789..5989)
                     /locus_tag="VNZ06_00450"
     CDS             complement(5789..5989)
                     /locus_tag="VNZ06_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VapB family protein"
                     /protein_id="HWW19246.1"
                     /translation="MPTKTISIETDAYELLAREKRDRTESFSRVIRRLFAERPALTAG
                     ELLDAMEPYKGKGAGRRRNKQS"
     gene            complement(6161..6577)
                     /locus_tag="VNZ06_00455"
     CDS             complement(6161..6577)
                     /locus_tag="VNZ06_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWW19247.1"
                     /translation="MPNASSSRTPHYLLSALLLGLIVVPALKSGAADTASPQAGDPDA
                     GAEVYRNICKECHGGSLAPPLRGVVGRHIASAPDFDYTDALLAKKDMTWTRENLDAFL
                     IDPQDWAPGTEMKKKELTAQNRADVIAYLASLTVAN"
BASE COUNT         1150 a         1962 c         2246 g         1289 t
ORIGIN      
        1 ggagtttttc tccagtgcct atggcttgcg tggtgccaga cagcgcgagc gtatcgaatg
       61 ggcgattgcg caatttgaac tgggggacca gttgcgcggt accagtgcgc aactgcccgg
      121 cggctacaag cagcggctgg cgatggcggc agcgttgctg cacgaaccgg agatcctgtt
      181 cctggatgag cccacgagcg gcgccgatcc gctggcgcgc cgcgccttct gggcgcgtat
      241 cacctcgctg gcgcaacagg gtgtcacggt catcgtcacg acgcacttca tggaggaggc
      301 cgagtattgc gatcgcattg cgatcatgga cgccggccgc atcctggcgc agggctcacc
      361 ggcgcaggtg cgggcgttgg gccacgcggc gagtgaacag ccgaccatgg atgacgcctt
      421 cgtctcgatc gtgacgcgag cgcgtcgcga tcgggctgcg cagcccgcat gagcccgagc
      481 gtccggccgt actggcgcat caagctacag cgcgtgcggg cactgatcat caaggaatcg
      541 cgtcagattg tgcgcgaccc ttccagtatc gcgatcggtg tcattctgcc gctgattctg
      601 atcctgctgt ttggctacgg cctgtcgctg gatgtgcgca atgtaccgat tgccgtcgtg
      661 atggatcagc cgtccaccca ggggcaggag ttgctcgcgg cgctgcagct gtcgccttat
      721 ttcaatccgc ggcaggtcgc gacgatgccg gcggccgaga atctcatggc gcacggtgag
      781 gtggatggca tcgtgcatct ggcgcaggag tttgatcgtc gcatgagcgc gggtgatgcg
      841 cagctacagc tgatcgtgca gggcggggat gccaaccggg cccgcatcat cgaggcctat
      901 gtgcaaggcg cggtgggtgt ctgggcggct catcgggcag cacaggggcg cggcggatcc
      961 gtggggccgg tcctcgtaca atcgcgcctg tggttcaatg atgcgaacga gagtcactac
     1021 tttctggttc cgggcctgat cgtgctggtc atgaccctga tcggcggact gctcaccgcc
     1081 atggtcgtcg cacgcgagtg ggagcgcggc acttatgagg cgctactggt gaccccggtc
     1141 cgcagcgaag aaatcctgct cggcaagact ctgccgtact ttgtactggc gctgatcggc
     1201 ttttcactgt gcgcgctgtc ggcacagttc ctgttccatg ttccgttgcg cggctcattc
     1261 tggatattgc tgttcgattc gatgctatac gtgctggttg ccttgggcat cggtctgttg
     1321 atctcctcgt gggtcaagag tcagctggtg gcgagccagc tcaccatgct ggtgacgttc
     1381 atgccggcct atattctctc cgggtttctg tttgatttgc gcagcatgcc gctggcggta
     1441 cgcttcgtga cctatctgct gcccgcgcgc tactacgtgg cgctgctgca gagcgtgttt
     1501 ctggcgggcg atgtctggtc agtcatcgtt cccaacaccc tggtgctggc gttgatggcg
     1561 gggttgctgg cactggccag ccgcgccgtc atgcgcaaac ggctggagtg attcgagtgc
     1621 tcgcgtcgat ctaccggata ctggccctga cgcgcaagga gctgctggcg gtgctcaagg
     1681 atcggcgcgg gcgcatgagc ctgctgatac cgccggtcgt tcaagcgctg atctttggct
     1741 atgctgccac ctacgacctg aatcacgtgc cgtatgccgt gttcgaccag agtcatagcg
     1801 gcgcctcgca gcagttgctg gcgcatatcg acgggtccgg cgtgtttcaa cgggtagcca
     1861 ctctgagcca ggcgcaggac atcgcacgcg aggtggattc gcgcaaggcg ctgctggcac
     1921 tggtcatcga ctcggatttc gagcgtcgga tgttcagtgg ccagggcgcg cagctgcagg
     1981 tcatcgcgga cggccgcaac tccaataccg ccggaaccgc catgggatat ctgggcgccg
     2041 tcgtcaagcg tttcaacacg caatggcgac gcgatcatgg gctggggccg ccgtcgctga
     2101 cgatcactgc acgcgcctgg tacaacccga atctcgagac gcgctggtac atgattcccg
     2161 gcatgatcgg aacgctgacg ctgctacaga cgctgatgct caccgcgatg tcggtggcgc
     2221 gcgaacgcga gcagggtacc ttcgatcagc tgctggtgac acccttccgg ccgtatgagc
     2281 tgatggcggg caaggctttg ccatccatgc tcgtcggaat catccaggcc accggcgtat
     2341 tgctggtcgc gcaattgtgg tttcgcattc cgttcgccgg atcctacctg acgctgtata
     2401 tcggactgct ggtgttcctg atggcagcgg tcgggatggg attgctggtg tccgcagcat
     2461 ccgccacgat gcagcaggcc atgctctatt cgatgctgct gatcatgccg ttcgcgctgc
     2521 tgtcagggtt aacaacacct gtgagcaaca tgcctgggtg gctgcagtat ctgaccgcga
     2581 tcaatccgtt gcgctacgcg atcgatatca cgcgccgcgt ctatcttgaa ggcgcgggac
     2641 ttgcgctgct gggaggggat ctgctgccgc tggcgatcgt cgcagccgcg accctgtcga
     2701 tttcttcctg ggtgttcgcg caccggatgc agtaatgaaa cgaggcgttc gtcggccgat
     2761 cggatggctg cgttggctgt gctgcggcct gatcgcaagt ggctgcagcg ttggccccaa
     2821 cttcgtgcgg ccgcaggcgc ggacgccgga acactggagc gcggcttcag cgccggcagc
     2881 ggccccgcgc gccagtcacc tcagtgaaga cggccagctg gccgagcgct ggtggctgca
     2941 attcaacgat accggcatgc aatccctgat cgagcgggcc ctcaaagcca atcttgatct
     3001 gagaatagcc atgcagcgcg tcgaggaggc gcgtgcacag cgcgatgtga ccggggcgca
     3061 ggcctggccg agactgagcg tgggcgccag ctacacccgg gcgcgattga gtcaaacgac
     3121 cccaaccgga tcgctgttca gttcggtcgg tcagatttcg ctgccgggcg gggcccatgt
     3181 gaatgtaccg ctcagctaca accagtttca gaccgacgcg gatgcttcct gggaaatcga
     3241 tctgtttgga cgggtgcggc gcgcgctcga agcggccaac gcgcagattc aggtatcgat
     3301 cgaagacgaa cacgccgtgc agctgacgct gatgtcggat gtggcgcaga gctacatcga
     3361 gttgcggggc gcacaattgc gcaaagcgac cgcggtcgag aacatcgcca cgttgagcga
     3421 gctgctggaa ctcacgcagc aacggcgcga tgccggtctc acgagcgatg aggacgtcag
     3481 ccgaagcgca gcgcagctgg atgcgacgcg gtccgacctt ccggccctgg agctgcagat
     3541 cgtcgaaggc atcaacgctc tgagcgcatt gctggctcgg gagccggatg cgctgcgcgc
     3601 ggaactcgag acggccgcgc cgattccggc cgtgccggaa tcggtcgcaa tcggtgtgcc
     3661 cgcggatctg gcgcgacggc ggcccgacgt tcgcgaagcc gaggccagtc tgcacgtcgc
     3721 caccgcgcag atcggggttg ccaccgcaga cctgtttccg cgcctggtgt tgagcgccca
     3781 gggcggattg cagtcgcagg cgctgagcca gcttggggag tgggcgagcc gttttggatc
     3841 gcttggtccg agctttcagt tgccgatctt cgatcgtggg caatggagga cggtgcatct
     3901 tgacaagctg cgggcgcagg agagcgcgct cgcctaccag cgcacggtca tccaggcgct
     3961 gcgggaagtg gccaatgcgc tggccgcctt caatgccgat caggatcgcc gtgtacagct
     4021 tgatcgagcg gtgagtgaaa gccgcgacgc actggcgctt gcgcgggagc ggtatcaaag
     4081 tggagtgacg aacttcatcg acgtgctcga cgcgcagcgc acgctgcagc agaatgagtc
     4141 ggcgctgatc gacgctatca gcgcgacgag cgtggatctg gtggcgcttt atcgcgcgct
     4201 gggaggaggg tggagcgatg cgatggatca ctagaattgc tctggcgctg ttgctcgtgg
     4261 tgggagtggc actgctgtcg ctggcgctgg tcgtcagtca caacggcgca tgcgtgagcg
     4321 ctccaggacc atccgcggat gccgagcgga tgaaagcgat cgaatatcgc tgctacggta
     4381 cggccgacgt gctcaaggtg gagacggtcg tacggcccgt cccagcggac aaccaggtgc
     4441 gcgtgcaggt gcgggcggcg tcgatcaatc cgctggattg gcactacctg cacggcacgc
     4501 cctacctgat gcgcctcagt gcgggaatcg gcacaccgaa ggatcccggg ttaggcgccg
     4561 acttcgcggg cgtggtcgag tcggttggca ggagcgtcac gcatttcaaa gccggtgacg
     4621 ccgttttcgg gcaggctgat ggtacgctcg ccgagttcgt gacgctgccc gaggacggga
     4681 gtctggcgag caagccggac aacatctcgt ttgaacaggc cgctgcgatc ccggtggccg
     4741 gcgtcaccgc gctgcaggcg ctgcgcgatc aggcgcacgt gctaccggga cagaaggtgc
     4801 tgatcaacgg cgcttcggga ggtgtgggaa cctttgccgt acagatcgcc aaggcgctcg
     4861 gcgccgaagt gactggcgta tgcagcacgc ataacgtcga gatggtgcgc tcgctcggtg
     4921 ctgatcatgt ggtggactac acgaaagagg atttcaccaa aagcgatcaa cgctatgacg
     4981 tggtcctgga caacgtggtc aatcattcac tgtgggacta tcggcatgtg ctcacgccca
     5041 agggcatcct ggtcatcgtc ggtggcggat cgtcgaacga gaatccatgg atcggagcgt
     5101 tccaggcgcc gctgaaggcg agcgtccagt cgctgttcgt cagcccacgt tgggtgatgt
     5161 tcttcgctga tgtgagcgcc aagaacctga cggcgatgca tgatctggtc gcgagcggga
     5221 aggtcacccc tgtcatcgac cgacattacg cgctggatga gaccgccacg gcgatgcgat
     5281 acctggaaca gggacacgcg cggggcaaga tcgtcatcga catcccgtag cagccagccg
     5341 aacctgggtt tcgcacgacc tgggtttcgc acgacgatgg tcgagggtgg cggctggctc
     5401 gcgttgcagc gccctagaat tccagatagt ccagtctcgg cacccgctcg aaggcgcgct
     5461 cgcgcccgat gagtgtagcg ccatagcaca gcgcggtcgc ggcgatccag gcgtcatttt
     5521 ccccgagtcg gcgcgaaagc gacgtctgca gcggcgctgt tctccaggca atggcgcgcg
     5581 acagctgaag cacgcggaaa tggctgacaa actcgttcag cgcctgcgga tcagtgaaac
     5641 cctcggcgaa ctcgccaagt gctaccacac tgatcgcaac tggcatcgat cggtgcattg
     5701 cgagaaccgc cgttgccacg ccgggttgat tcgctgcaat ctctcgttcg aactcgatga
     5761 ggaagctcgt gtcaagcagc agcatgcgtc aggattgctt gttgcgcctg cgacccgcgc
     5821 ctttgccctt gtaaggctcc attgcatcga gtagctcgcc ggcagtcagt gccgggcgct
     5881 ccgcgaacag ccgccggatg acacgagaga acgattcggt ccgatcgcgc ttctcgcgag
     5941 cgaggagttc gtaggcatcc gtttcgatcg aaatggtctt cgtgggcata cacagaccat
     6001 acacgggccg tgtacgtcag gtcaacctcg tgcggcagtt gcctcgatac gtcgatcggt
     6061 tggcgggtcg tgtggggtct gcgaccaatg gcaatagggc ggggcgaaat gctctgaggg
     6121 cggacgggcg ggagctcggc ggcggctcag ccgccacgaa tcaattcgcg acggtcagac
     6181 tggccagata ggcgatgacg tcggcgcggt tctgcgcagt cagctccttc ttcttcattt
     6241 ccgtaccggg cgcccaatcc tgcggatcga tcaggaacgc gtccagattc tcgcgagtcc
     6301 aggtcatgtc cttctttgcc agcagcgcgt cggtgtagtc gaagtccgga gcgctggcga
     6361 tatgacggcc gacgacgcca cgcaggggag gtgccagcga tccaccgtga cactccttac
     6421 agatgttgcg atacacctcc gcacccgcgt ccggatcgcc ggcctgcggc gaggcggtat
     6481 cagcggcacc gctcttcagg gcaggaacga cgatcaggcc tagcaacagg gcgcttaaca
     6541 ggtagtgcgg tgtcctgctg gagctggcgt tgggcatttc tgatgctgtg cgaggttgga
     6601 tcggcgggtg aattcaagtc tacgggaacg tcggcggcgg cgccaat
//
LOCUS       DATMRM010000014         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_627831, whole genome shotgun sequence.
ACCESSION   DATMRM010000014 DATMRM010000000
VERSION     DATMRM010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627831"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VNZ06_00460"
     CDS             complement(<1..462)
                     /locus_tag="VNZ06_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HWW19248.1"
                     /translation="MATHRSPDPVPQALLPHAAIERFAEHAVLMSGYALRQARELFDA
                     VLRVAEAAPWRHMVTPGGLTMSVAMTNCGQVGWVTDRHGYRYTDTDPEGGRYWPAIPA
                     RMKALASKAADAAGFADFEPDACLVNRYEPGNRLSLHQDRNERDFSAPVVSV"
     gene            533..913
                     /locus_tag="VNZ06_00465"
     CDS             533..913
                     /locus_tag="VNZ06_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HWW19249.1"
                     /translation="MARRIKVREFLGSSARRHARSPGSEPGENPALQAYWDVVAMIPQ
                     GRVSTYGEVARMAGFPGRARQAGAALRRVPPGMNLPWHRVVGAGGRIVFPKSSSHFRE
                     QARRLRSEGVKVTAGRVASEALLR"
     gene            complement(917..>2731)
                     /locus_tag="VNZ06_00470"
     CDS             complement(917..>2731)
                     /locus_tag="VNZ06_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HWW19250.1"
                     /translation="LTVESTGKIERLERKVYRILRPQGSAKGVVHLDFDAQSRITHMQ
                     GWSLATDGKVREVSDRDAADTALPGIMNGELESDLRARLLHIPAAEPGSLIAYETQQE
                     LRPYVLTDDWSFQETVPVREAHYSLQLPPGWQYKATWIHHDEESPTAAGNSRRQWIVR
                     DVKGIRTERRMPSFDAVAARLTIALLPPSGKGQGFQNWNEFGSWYQTLTQGRRDASPD
                     IKQKVLELTTSATTTLGKEQALAGFVQSDIRYVAIELGIGGLQPHPAKDVFSHRYGDC
                     KDKATLLSSMLKEIGVDSYYVMVNTQRGAIDESTPANPAFDHAILAIQLPPELNDPSL
                     LAVINDPKLARLLLFDPTDSYTPLGQLSGTLQNGFGLLVAPDGGQLIKLPQMPPSTNS
                     IQRTAHLQLDEAGNLQGEVHELRTGDEAAFERSTVVGATEATDQIKSVESVMADSFPT
                     FQLTHASIGNLHASNRPLEWNYTLEVPHYAKLSGGLLTVRPRVIGSKSTGLLETREPR
                     RYDIDFDGPQRDTDTFEINLPEGYGVDELPPPVNEEHAFGSYHSKTELSGHILKYTRT
                     YEIKNVTVPAAQADELKKFFREIASDERMVAVLKKNGQ"
BASE COUNT          465 a          818 c          889 g          559 t
ORIGIN      
        1 taccgacacc accggcgcac tgaagtcgcg ttcattgcgg tcctgatgca ggctcaggcg
       61 gttgcccggc tcatagcgat tgaccagaca ggcatcgggc tcaaagtccg caaacccggc
      121 cgcgtccgcc gccttgctcg ccagcgcttt catgcgagcg gggatcgcgg gccagtaccg
      181 accgccctcc ggatccgtgt cggtgtaacg atagccatgt cggtcggtga cccagccgac
      241 ctggccgcaa ttcgtcattg cgacggacat ggtcaggccg cccggggtca ccatatgacg
      301 ccatggggca gcctctgcca cacgcagtac tgcgtcgaac agctccctgg cctgcctcag
      361 cgcgtagcct gacatcagca ccgcatgctc cgcgaaacgc tcgatcgccg cgtggggcag
      421 caatgcctga ggcacgggat cgggcgagcg gtgggtagcc atacgagcag gataagctcg
      481 cgcgatgccg cgtcaattcg agagcgaccg cggtatggca gaatgatggc ggatggcgcg
      541 caggatcaaa gtccgcgagt ttctgggatc cagcgcccgc aggcacgcgc gctcgcctgg
      601 ctccgagccc ggcgaaaatc cggcgctgca ggcatattgg gatgtggtcg cgatgatccc
      661 gcaggggaga gtgtccacct acggtgaggt cgcgcgcatg gccggatttc cggggcgtgc
      721 gcggcaggcc ggcgcggcgc tgcgaagggt gccgcccggc atgaatctgc cctggcatcg
      781 cgtggtcggt gccggcggac gcattgtgtt tccgaaatcg tccagccact tccgtgaaca
      841 ggcgcggcgg cttcgcagtg agggcgtgaa ggtcaccgct ggccgggtcg ctagcgaagc
      901 actgcttcgc tgatcgctac tgcccgttct ttttcagcac cgcgaccatg cgctcgtcgc
      961 tggcgatctc gcgaaagaat ttcttgagtt catccgcctg tgcggccggt acggtgacat
     1021 tcttgatttc gtaggtgcgg gtgtacttca agatgtgacc gctcagctcc gttttactgt
     1081 ggtaggaacc gaacgcgtgc tcctcgttga ccggcggcgg caattcgtcc acgccgtagc
     1141 cctcgggcag gttgatctcg aatgtgtcgg tgtctcgctg cggaccgtcg aagtcgatgt
     1201 cgtagcgacg cggttcgcgg gtttcgagca agccggtcga tttgctgcca atgacacgcg
     1261 ggcgtaccgt caacaggccg ccactcaact tggcgtagtg cggtacctcg agagtgtaat
     1321 tccattccag cggtcggttg gaggcatgca gattgccaat gctcgcgtgc gtcagctgaa
     1381 aggttgggaa tgaatcggcc atgaccgact cgacggattt gatttgatcg gtcgcctcgg
     1441 tcgccccgac caccgtactg cgttcaaatg cggcctcatc cccggtgcgc agttcgtgaa
     1501 cctcaccctg taggttgccg gcctcatcca gttgcaggtg cgctgtgcgc tgaatggaat
     1561 tcgtgctggg gggcatctgc ggcagtttga tgagttggcc cccgtcaggg gcgaccagca
     1621 atccgaagcc gttctgcagt gtgcccgaga gttgtcccag cggggtgtag ctatcggtcg
     1681 gatcaaacag tagcaggcga gcgagctttg gatcgttgat taccgccaga agactcggat
     1741 cgttcagctc cggcggcaat tgaatggcga ggatcgcgtg gtcgaaggca gggtttgcag
     1801 gggttgattc gtcgatggcg ccgcgctggg tattgaccat cacgtagtac gaatcgacgc
     1861 caatctcctt cagcatcgag ctcagcagtg tcgccttgtc cttgcagtca ccgtaacgat
     1921 gggagaacac gtccttggct ggatgcggtt gcaacccgcc gatgcccagt tcgatcgcga
     1981 catagcggat atcgctctgc acgaaaccgg ccaatgcctg ctctttgccg agcgtcgtcg
     2041 tggctgaagt cgtgagttcg aggacttttt gtttgatgtc cgggcttgca tcgcgtcgtc
     2101 cctgcgtcaa ggtctggtac cagctgccga actcattcca gttctgaaag ccctgaccct
     2161 tgccgctggg aggtagcagc gcgatggtca aacgggccgc aaccgcatcg aatgacggca
     2221 tccgacgctc ggtgcggatc cctttgacgt ccctgacgat ccattgccgg cgggagttgc
     2281 ccgcggcggt cggtgattcc tcgtcgtgat gaatccaggt ggccttgtac tgccagcctg
     2341 gcgggagttg cagactgtag tgcgcttcgc gcacgggcac tgtttcctga aaggaccagt
     2401 cgtcggtgag cacatagggc cgcagttcct gctgggtctc ataggcgatc aggctgccgg
     2461 gctcagccgc tggaatgtgc agcaaacgag cgcgcaggtc ggactcgagc tcaccgttca
     2521 tgatgcccgg cagagcggtg tcggcagcgt cgcgatcgga gacctcgcgc actttgccgt
     2581 cggtcgccag cgaccagccc tgcatatgag tgattctgct ctgtgcgtcg aaatccaggt
     2641 gcaccacgcc cttcgcgctg ccctggggcc gcagaatccg gtagaccttg cgctcgagac
     2701 gctcgatctt gccggtggat tcgaccgtta g
//
LOCUS       DATMRM010000015         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3416680, whole genome shotgun sequence.
ACCESSION   DATMRM010000015 DATMRM010000000
VERSION     DATMRM010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3416680"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VNZ06_00475"
     CDS             complement(<1..1044)
                     /locus_tag="VNZ06_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HWW19251.1"
                     /translation="MSSGTEGVVAPSVARTGPAASTRTFLGHPLGLTTLFFVEMWERM
                     SFYGMRALLVLYFVEQVARGGLGLDDRTAVSIYGLYVGGTYIASLPGGWFGDRLLGGQ
                     RAVLIGGIIIAFGHVTLGVAPTKEVFFLGLFVIVLGTGLLKTNSSAIVAQLYPPGDAR
                     RDAGFTIFYVGVNLGGWLGPLICGWLALKYGWAAGFFSAAVGMTAGVIQMLWGRSLLG
                     DAGKAPSGPASRGAVRGALIAVIAAVLLVALCFSGLIHLDPVYLASVSTEAVVAIAVL
                     YFAYLLIGAGLTAIERQRVGVMMLLFIASALFWAGFEQSGSSLTLFAQRYTERHAFGA
                     EVPAAWFQSLEALF"
     gene            complement(1041..1811)
                     /gene="tatC"
                     /locus_tag="VNZ06_00480"
     CDS             complement(1041..1811)
                     /gene="tatC"
                     /locus_tag="VNZ06_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HWW19252.1"
                     /translation="MSEDNEEALPEGTLISHLLELRSRLLKAFIAVLIAFCPAWYYSN
                     QVFDFISQPLVAKLPPGQKLIATTVTATFTAPLKLSFVVGLLVAMPYVLYQIWAFVAP
                     GLYKREKRFAVPLLASAILLFYVGVAFSYFFVFPLAFKFFVATTPQNVVLMADITNYL
                     KFVLTLFVAFGVAFETPVAVVLLVLTNLLTLEKLRSIRGYVLIAVFIIAAILTPPDVL
                     SQFSMAVPMYLLYEGGILFAAILRRSNRAVEVREEESA"
     gene            complement(1795..2112)
                     /gene="tatB"
                     /locus_tag="VNZ06_00485"
     CDS             complement(1795..2112)
                     /gene="tatB"
                     /locus_tag="VNZ06_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HWW19253.1"
                     /translation="MFGVDFSEIVVIFVIALIVLGPQKLPKLAQTIGRWLGRARSMAR
                     QFREQLEQEAEQMQRATDLKTHMKPPSRPVPTPGPDPDIASAHMTPQTDTPAPAESHE
                     RGQ"
BASE COUNT          487 a          697 c          696 g          352 t
ORIGIN      
        1 gaacaacgct tcgagcgact ggaaccaggc cgcgggaact tcggcgccga acgcgtggcg
       61 ctcggtgtaa cgctgggcaa agagggtcag cgacgagccg ctctgctcga agccggccca
      121 aaagagtgcc gaggcgatga acagaagcat catcactccg acgcgctggc gctcgatggc
      181 ggtcaaaccg gcgccaatga gcagataggc aaaatacagg accgcgatcg ccaccaccgc
      241 ctcggtgctg acactggcca gatagaccgg atcgaggtga atgaggccgc tgaaacacag
      301 cgccaccagc agcactgcag cgatgaccgc gatgagtgcg cccctcaccg caccgcgact
      361 cgccggtcca gagggcgcct tgccggcatc gcccagcagc gaacgtcccc acagcatctg
      421 gatcactccc gcggtcatgc ccacggcggc actgaagaaa ccggccgccc agccgtactt
      481 gagtgccagc cagccgcaga tcagcggtcc cagccatccg cccaaattga cgccgacata
      541 gaagatggtg aatccggcgt ctcgccgtgc gtctcccggc ggatagagct gggcgacgat
      601 ggcgctggaa ttggttttca gcaggcctgt cccgagcacg atgacgaaca atccgagaaa
      661 gaacacctct ttggtgggcg cgacccccag ggtgacgtga ccgaacgcga tgatgatgcc
      721 gccgatcagg acggcgcgct gcccgccgag cagtcggtca ccgaaccatc cgccgggcag
      781 actggcgatg taggttccac ccacgtacaa accatagatc gacaccgccg tgcgatcgtc
      841 cagtcccagt ccgccgcgcg ccacttgttc gacgaaatac agcacgagca gggcgcgcat
      901 gccgtagaaa ctcatgcgct cccacatttc gacgaagaac agcgtggtta ggccgagcgg
      961 atgacccaga aaggtacgcg ttgacgcagc tgggccggta cgcgccaccg acggggctac
     1021 caccccttca gtgcccgaac tcacgccgat tcttcttctc ttacttcgac cgcccggttg
     1081 ctacgacgca ggatcgcggc aaacagaatg ccaccctcat acagcaggta catcggtact
     1141 gccatcgaga actgcgacaa cacatcgggc ggcgtcagga tcgccgcgat gataaagacg
     1201 gcgatcagca catagccgcg gatcgagcgc agtttttcca gcgtcagcag gttggtcaat
     1261 accagcagca ccactgcgac cggtgtctcg aaggccacac caaacgccac gaacagcgtg
     1321 agcacgaatt tcagatagtt ggtgatgtcg gccatcagaa ccacgttctg cggtgtggtg
     1381 gcaacgaaga atttgaacgc cagggggaat acgaagaaat acgagaatgc cacgcctacg
     1441 tagaacaaaa ggattgcgga tgccagcagc ggcaccgcga agcgcttctc gcgcttgtac
     1501 aaacccggcg cgacgaaggc ccagatctga tacagcacat agggcatcgc gaccagcagg
     1561 ccgacgacga atgagagttt cagcggcgcg gtgaaggtcg cggtgacggt ggtcgcgatc
     1621 agcttctgcc cgggtggcag cttggccacc aacggctgcg agatgaaatc gaaaacctgg
     1681 ttgctgtaat accaggcggg acagaacgcg atgaggaccg cgatgaaggc cttcagcagc
     1741 ctcgaccgca gctcgagcaa atgcgaaatc agcgtgcctt ccggcagcgc ctcctcattg
     1801 tcctcgctca tgcgattccg ccggcgcagg cgtgtcggtc tgcggggtca tgtgggccga
     1861 agcgatgtcg ggatccggtc ctggggtcgg caccggccgc gagggcggct tcatgtgcgt
     1921 cttcaggtcc gtcgcacgct gcatctgctc ggcttcctgc tcgagctgtt cgcgaaactg
     1981 tctggccatg gagcgcgccc gcccgagcca gcggccgatc gtctgtgcaa gcttgggtag
     2041 cttctgcgga cctaagacga tcaaagcgat gacgaagatc acgacgatct cggaaaagtc
     2101 gacgccgaac atggcgcgcg agggatcagt gcgcttaggc gtgcgaggcg ggtttgctct
     2161 caggggtcgc ctgaggcttg gctgcggcca cgtccgggaa ctcggcgtcc ttggatgcgg
     2221 gtccctgaat ct
//
LOCUS       DATMRM010000016         7102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1249245, whole genome shotgun sequence.
ACCESSION   DATMRM010000016 DATMRM010000000
VERSION     DATMRM010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249245"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /gene="glmS"
                     /locus_tag="VNZ06_00490"
     CDS             complement(<1..1419)
                     /gene="glmS"
                     /locus_tag="VNZ06_00490"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HWW19254.1"
                     /translation="MCGIVGYIGKRDAVPILTEGLRRLEYRGYDSAGVAVAAGGALRI
                     SKCKGKVRELEAQLPKRLKGAPGIAHTRWATHGEPSDINAHPHPDAAERFAIVHNGII
                     ENADALRAQLQATGVVFRSATDSEVLAHLIAANPGVTLAEGVRTALKFIAGTYGIAVI
                     DSQRPDTLVVARNGSPVVIGIGEHEMFVASDAGALVRHTQSVVHLDDGEVAQIRADGF
                     ETMTLDGGHTSKTPLTLTWADEAYTKGSFAHYMRKEISEQAEAVRRTLSGRLEPRFQT
                     SHLGGIELAARDLLEVRRLKVLGCGSAYIAGAMGAHMIEQLARLPCHAESAAEFRYRN
                     PVIEPDTLYIAVSQSGETFDTLAAVQEIKRKGGRVLGVINVVGSTIARECGRGIYIHA
                     GPEIAVVSTKTFTCTAVAFALLALYLGRLRDLSPAQGERLLAALTALPEQIERILARE
                     AEIARLAQAFSGCEHAYFVGRGS"
     gene            complement(1455..1727)
                     /locus_tag="VNZ06_00495"
     CDS             complement(1455..1727)
                     /locus_tag="VNZ06_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19255.1"
                     /translation="MSDLAGFEDLIARLARSSRLSERETTHVVEEVLAFLNDSIEQFV
                     RRRHRELQREGLRNPQIYLRVAQEALQRRFRTEALSTRQIRRLVYG"
     gene            complement(1881..2573)
                     /locus_tag="VNZ06_00500"
     CDS             complement(1881..2573)
                     /locus_tag="VNZ06_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19256.1"
                     /translation="MHSGLLALLAWVGAADCACAAPPPWPDTPVSRLEALALLQTLNA
                     ALLSHDSATLTLEQWCGTHHIADPAQVIARRVAAVDKPAGDEQRALLLVSATEPVRYR
                     RVQLTCGGVVLSQADNWYVPARLSTQMNAELEHSDTPFGKVVRSMHFSRHTLSSKLLW
                     QPMPEGWDSGRAVVPGKGRLNVPAAVLEHRAVLTREDGTPFSEVVETYTGNVLAFPMP
                     PVAGARCDGAKK"
     gene            complement(2584..3426)
                     /locus_tag="VNZ06_00505"
     CDS             complement(2584..3426)
                     /locus_tag="VNZ06_00505"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWW19257.1"
                     /translation="MERDINNDIKTIDHFKTEAIDIAIKFGPKILVAILILVVGSMVG
                     GWLARASERGLTRLEMEPPVRLLLVRLLRIVVFMLFLILALQNLGVELLPLIAGLSIA
                     GAGIALATQGVLSNVVAGLTIIFTKPYRVGEYIEIVGVQGLVQQISVFSTTLLHSDRS
                     RVVVPNRKIVGEILHNYGQIRQAEIMVGVGYESDLPVALAGMRELVTANQRVLRDPAP
                     LVQVVTLGENAVQIAVRPWVAVDDFGGVVDELSRAIIEDCRKRGVSIPFPQHEVRLIG
                     ADSK"
     gene            3480..4460
                     /locus_tag="VNZ06_00510"
     CDS             3480..4460
                     /locus_tag="VNZ06_00510"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HWW19258.1"
                     /translation="MQSAIPEFAILGAGAMGSIVAAHLARAGHSVVVLARAQRARDIE
                     QHGLRIRGLIEFTQRVPVISDPAMFRGAAVLILGTKTHGTDAALAPIRGADIGVAFSM
                     QNGMMKNDQLINALGRQRVLGSLADASGEVLPSGEVLFTRNGMLYLGELDGRDSARAH
                     KIASSIAAAGVNTSAVANILTLEWSKFASWVGMMALSVTTRAYSWKFLIDPNTALVVV
                     RLVREIGALAAAHDVQLSDRSTLPVASLCQSSEAQAVARLDELGRHMQHSAPQHRMST
                     LQDLNAGRTLEVEETLGFALREAQRLKVPTPLLDAMYHLIAGIDQIQRMS"
     gene            complement(4481..5437)
                     /gene="egtD"
                     /locus_tag="VNZ06_00515"
     CDS             complement(4481..5437)
                     /gene="egtD"
                     /locus_tag="VNZ06_00515"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HWW19259.1"
                     /translation="MLDVLHNRVGQADAFRADVLSGLLQQQKTLPARWLYDDRGSELF
                     ELITGLPEYYLTRTETAILNTRAQEIARFCGERAIMLEYGAGAGIKTEILIHALASPR
                     LYVPIDIAGDFLDQTVTRFRRRFPNLLTKSIVADFTADFDLPSWMPTHHNGLRVAFFP
                     GSTIGNLNAAEVAGFLQRVRRHVGGDGRMVVGVDMRKDLRILIPAYADAAGITAQFNL
                     NLLRRINRELAGNFVLDAFRHEARWNETESAIEMHLVSGVRQKVMIGGNRFAFEAGES
                     IHTESSRKYAPAAFEAIALEKGWRVGHRWTDRAGLFSVFGLE"
     gene            complement(5437..6714)
                     /gene="egtB"
                     /locus_tag="VNZ06_00520"
     CDS             complement(5437..6714)
                     /gene="egtB"
                     /locus_tag="VNZ06_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HWW19260.1"
                     /translation="MSAAPLFSSSLGLSELTLLYRGVRQVTSALSAPLSDADATVQSM
                     PDASPAKWHLAHTTWFFETMVLKPHAAGYRVFDEHFNYLFNSYYEAAGARQPRSRRGM
                     LTRPTLQAVHAYRAHVDAALDRYLASAPPQDVAALIELGCHHEQQHQELLLTDILHLF
                     AQNPLRPAYKAPSPLPVESSELAPCAFPYFEGGLIEIGHEGPGFAFDCEGPRHRAWVE
                     PYRLGERLVTNREWIEFIADGGYRSPLLWLSTGWAKVCEEGWEMPLYWEARDGEYWNM
                     TLRGAQPLLLTEPVTHVSYFEADAFAAWAKKRLPSETEWEHAANSASLDSNFLESGRL
                     RPKPVIGQAKGLTQMFGDVWEWTRSAYLPYPRFRANDGAVGEYNGKFMSGQYVLRGGS
                     CLTPESHIRASYRNFFPPDARWQFTGVRLAEDA"
     gene            complement(6728..>7102)
                     /locus_tag="VNZ06_00525"
     CDS             complement(6728..>7102)
                     /locus_tag="VNZ06_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19261.1"
                     /translation="NSVLIGTIAGVLGILANAFAPKQVFAFLINAAGALIVFVYVLIC
                     LAQIRLRRQRQRAGQPEPALQMWWFPWASYAAIAGMIAVLVAMAVTPDTQSQFYVSVL
                     ALAIAAGACFLVQRRRRESAPH"
BASE COUNT         1339 a         2403 c         2106 g         1254 t
ORIGIN      
        1 tgatccgcgg ccgacgaaat aggcgtgttc acaaccggag aacgcctgcg ctaaccgcgc
       61 aatctccgcc tcgcgcgcga ggatgcgctc gatctgttcc ggcagggccg tgagcgcggc
      121 caacagccgc tcgccctgcg ctggcgacag atcccgcaga cgacccagat acagcgccag
      181 cagtgcgaac gccaccgcgg tacaggtgaa cgtcttggtt gacaccaccg caatctcagg
      241 tcccgcgtga atatagattc cgcggccaca ctcgcgcgcg atcgtgctgc cgaccacgtt
      301 gatcacgccc agtacgcgtc cacccttgcg cttgatctcc tggaccgccg cgagcgtgtc
      361 gaaggtctcg cctgactggc tgaccgcgat atagagggtg tccggttcga tcaccggatt
      421 gcgataacga aactccgccg ccgactccgc atgacacggc agccgcgcca gctgctcgat
      481 catgtgggcg cccatcgcgc cggcgatata ggccgagccg catcccagga ccttgaggcg
      541 cctgacttcc agcagatcgc gcgccgcgag ttcgatgccg cccagatggc tggtctggaa
      601 tcgtggttcg agcctgccgc tgagcgtgcg ccgcacggct tcggcctgct ccgaaatctc
      661 ctttcgcatg tagtgcgcga acgagccctt ggtgtaagcc tcatccgccc aggtcagcgt
      721 gagtggggtt ttcgaggtat ggccgccatc gagcgtcatg gtctcaaagc catccgctct
      781 gatctgcgcg acttcaccat catccaggtg caccacgctc tgcgtatgcc gcaccagcgc
      841 accggcgtcc gacgcgacaa acatctcgtg ctcaccgata ccaatgacca ccgggctgcc
      901 attgcgcgcc acgaccaggg tatccggtcg ctgcgagtcg atgacggcga tgccataggt
      961 gccggcgatg aacttcagtg ctgtgcgcac gccttcggcg agcgtcacgc cgggattggc
     1021 ggcgatcaga tgcgccagca cttccgaatc ggtggccgag cgaaacacca cgccggtggc
     1081 ctgcaactgc gcgcgcagcg catccgcgtt ctcgatgatt ccgttgtgga cgatcgcgaa
     1141 gcgctcggcc gcatccggat gcggatgcgc attgatgtcg ctcggctcac catgcgtcgc
     1201 ccatcgcgtg tgggcgatgc cgggtgcgcc tttgagacgc tttggcagct gcgcctcgag
     1261 ctctcgcacc ttgcccttgc acttgctgat gcgcagcgct ccgccagcgg ccaccgccac
     1321 acccgccgag tcatagccgc ggtactcaag ccggcgcagg ccctccgtca ggataggaac
     1381 cgcatcgcgc ttaccgatat agccaacaat cccgcacata ttcgagcact cccgatgaat
     1441 cgtttgactt cgaatcagcc gtaaaccaaa cgtcggatct gtcgcgtgct caaggcctcg
     1501 gtgcgaaagc gtcgttgcag cgcctcctgc gccacccgca gatagatctg cggattgcgc
     1561 aacccctcgc gctgcagctc acggtgtcgc cgccgcacga attgctcgat cgagtcattc
     1621 agaaaggcga ggacttcctc gaccacatgg gtggtctcgc gttcgctcaa gcgactggaa
     1681 cgggccagtc gtgcaatcag gtcttcgaat cccgctaggt ccgacatgcc ggcgattctt
     1741 gagtttggcg cgcgcgatgt cgattattat gcccttattc gggcaaaata tgtgagtcac
     1801 tgctgtcggg cgctcgtcca tgcgagggag agggacactg cgaaaactaa gatatatctt
     1861 agatatattt taattctgga tcattttttc gccccatcac atcgcgcgcc cgcgacgggc
     1921 ggcatcggaa aggccagcac attgccggta taggtctcaa cgacctcgct gaagggggtg
     1981 ccatcttccc gtgtcagtac ggcgcggtgc tccagcaccg ctgccggaac gttcagccgc
     2041 cccttgcctg gcaccaccgc ccgcccgctg tcccaccctt cgggcatcgg ctgccacagc
     2101 aacttcgatg acaacgtatg ccgagagaaa tgcatcgagc ggacgacttt gccgaacggc
     2161 gtgtcgctat gctccagctc ggcgttcatc tgtgtgctga ggcgggccgg cacgtaccag
     2221 ttgtcggcct gtgacagcac cacgccgccg cacgtcagtt gcacgcgccg gtagcgcacc
     2281 ggctcggtgg cgcttaccag cagaagcgcc cgctgctcat cgcccgcggg cttgtccact
     2341 gccgcgaccc gtcgcgcgat cacctgcgcc ggatccgcta tatgatgcgt accgcaccac
     2401 tgctccaggg tcagcgtcgc gctgtcgtga ctgagcaacg cggcattcag cgtctgcagc
     2461 agcgccagcg cctccagacg cgagacgggc gtgtccggcc atggcggcgg cgcggcgcag
     2521 gcacagtccg ctgcgcccac ccacgccagc agcgcgagta gccccgaatg cacgacaccg
     2581 acgctacttg ctgtcggcac cgatcagccg gacctcgtgt tgcgggaacg gtatcgagac
     2641 accgcgcttg cggcagtcct cgatgatcgc gcggctgagt tcatccacca cgccaccaaa
     2701 gtcatccacc gctacccatg gccgcacagc gatctgtacg gcgttctctc ccagggtcac
     2761 gacctgcacc agtggcgccg gatcgcgcag cacacgctga ttggccgtca ccagctcgcg
     2821 catgcccgcc agcgcgaccg gcagatccga ttcgtagccg acaccgacca tgatctccgc
     2881 ctgccggatc tgtccgtagt tgtgcagaat ctcaccgacg atcttgcggt tgggcaccac
     2941 cacgcgagag cgatcactat gcagcagcgt ggtgctgaac accgagatct gttgcaccag
     3001 gccctgcacg ccgacgatct cgatgtactc acccacccga tagggtttcg tgaagatgat
     3061 cgtcagcccg gccaccacgt tgctcaacac cccctgcgtg gccagcgcga tgcccgcacc
     3121 ggctatgctc aaaccggcaa tcagcggcag cagttcgact cccagattct gcagcgcgag
     3181 gatcaggaac agcatgaaca ccacgatccg caacaaccgc accagcagca gccgcaccgg
     3241 tggctccatc tccagtcgcg tgaggccgcg ctcactggcg cgcgccagcc agccgcccac
     3301 catggagcct acgaccagaa tcagaatcgc caccagaatc ttcggaccaa acttgatcgc
     3361 aatatcgatc gcttcggtct tgaagtgatc gatggtcttg atgtcgttat tgatatcgcg
     3421 ttccatggcg cctcctgctc tggccgaagc tattgtaacg gctcggctat actgagccga
     3481 tgcagtctgc cattcccgag ttcgccattc tgggtgcggg cgcgatgggc tcaatcgtcg
     3541 ccgcgcatct ggcgcgcgcg ggacattcgg tggtggtgct ggcgcgcgcg cagcgcgccc
     3601 gcgacatcga acagcacggc ctgcgcatcc ggggactcat cgagttcacg cagcgcgttc
     3661 ccgtcatcag cgatcctgcc atgtttcgcg gcgccgctgt cctgatcctc ggcaccaaga
     3721 cgcatggcac cgacgcggcc ctggcaccga tccgcggcgc tgacatcggc gtcgccttct
     3781 cgatgcagaa tggcatgatg aagaacgatc agctcatcaa tgccctcggc cggcagcgcg
     3841 tgctcggctc gctcgctgat gccagcggtg aggtgttgcc gtccggcgag gtgctgttca
     3901 ctcgcaatgg catgctgtac ctgggtgagc tggatgggcg cgacagcgcg cgcgcacaca
     3961 agatcgcctc gagcatcgct gccgcgggcg tcaacaccag cgccgtggcc aatattctca
     4021 cccttgaatg gtccaagttc gcctcgtggg ttggaatgat ggcgctgtcg gtcacgacac
     4081 gcgcctacag ttggaaattt ctaatcgacc cgaacacggc gctggtggtg gtgcggctgg
     4141 tgcgcgagat cggcgcgctc gcggccgccc atgacgtgca actctccgac cgctcgacgc
     4201 taccggtcgc ctcgctgtgc cagagcagcg aagcgcaggc ggtcgcgcgc ctcgacgaac
     4261 tcggacgcca catgcagcac tcggcgcccc agcaccgcat gtcaacgctg caggacctca
     4321 atgccggtcg cacgctcgaa gtcgaggaaa ccttggggtt cgcgctgcgc gaagcgcaac
     4381 gcctcaaagt gccgacgcca ctgctcgatg cgatgtatca cctgatcgcc ggcatcgacc
     4441 agatccagcg catgagctga accgacttgc ggcgctcgct ctactccaga ccgaataccg
     4501 agaacaggcc cgcgcgatcg gtccagcggt gtccgactcg ccagcctttc tcgagcgcga
     4561 tcgcctcaaa agccgccggc gcatacttgc gcgaactctc ggtatgaatg ctctcaccgg
     4621 cctcgaacgc aaaccggttg ccgccaatca tcaccttctg gcgcacgccg ctgaccagat
     4681 gcatctcgat cgccgactcg gtctcgttcc agcgcgcctc gtgccggaac gcgtcgagca
     4741 cgaagttgcc ggcaagctcg cggttgatcc gccgcagcag gttcagattg aattgcgccg
     4801 tgataccggc ggcgtcggcg taggccggga tcaggattcg caaatccttg cgcatatcga
     4861 caccgaccac catgcgaccg tcgccgccca cgtgccgacg tacccgttgc aggaatccgg
     4921 ccacctcggc cgcgttcaaa ttgccaatgg tcgaacccgg aaaaaacgcc acgcgcaaac
     4981 cgttgtgatg cgtcggcatc cacgacggca gatcaaagtc agcggtgaaa tccgccacga
     5041 tcgacttggt cagcagattc ggaaatcggc gccggaaacg cgtcaccgtc tggtcgagga
     5101 aatccccggc aatgtcgatc ggtacataca gccgaggcga ggccagcgca tggatcagga
     5161 tctcggtctt gatgccagcg cccgcgccat actccagcat gatcgctcgc tcgccacaaa
     5221 agcgcgcaat ttcctgcgcg cgcgtgttca ggatcgcggt ttcggtacgc gtcaggtagt
     5281 actcgggaag gcccgtgatc agctcgaaca gctcactgcc ccggtcatca tacagccagc
     5341 gtgcgggtag ggtcttttgt tgctgtagca gtccggacaa cacatcggcg cgaaacgcgt
     5401 cggcctgtcc aacccggtta tgcaggacat caagcatcag gcatcctccg ccaatcgaac
     5461 cccggtgaac tgccagcgcg cgtcgggcgg gaaaaagttt cgatagctcg cccggatgtg
     5521 actctcgggc gtcagacacg aaccgcctcg cagcacgtac tggccggaca tgaatttccc
     5581 gttgtattcg ccgaccgccc catcattggc acgaaagcgc gggtacggca gataggcgct
     5641 gcgggtccac tcccacacgt cgccgaacat ctgcgtcagg cccttcgcct gccctattac
     5701 cggtttgggg cgcaagcggc cggattcaag gaaattgcta tccagcgatg cgctattcgc
     5761 cgcatgctcc cattcggttt cactgggaag gcgctttttc gcccaggcag caaacgcatc
     5821 ggcctcaaaa taactcacgt gtgtgacagg ttctgtcagc aataacggct gcgccccgcg
     5881 cagcgtcata ttccagtact cgccgtcccg cgcctcccaa tagagcggca tctcccagcc
     5941 ttcctcacag accttggccc aacccgtgga tagccacagc agcggtgagc gatagccacc
     6001 gtcggcgatg aactcgatcc attcccggtt ggtgaccagg cgctcgccca gtcgatacgg
     6061 ctccacccat gcccgatggc gcggaccttc gcagtcgaat gcaaatcccg gaccctcgtg
     6121 cccgatctcg atcagcccgc cctcaaagta cgggaacgcg caaggcgcca gctcgctgct
     6181 ctcgaccggc aacggactgg gcgccttgta ggccggacgc agcgggttct gcgcaaacag
     6241 atgcaggata tcggtcagca gtagttcctg atgctgctgc tcgtgatggc agcccagttc
     6301 gatcagggcg gccacgtcct gagggggagc gctggccaga tagcgatcga gcgcggcatc
     6361 gacatgcgcg cgataggcgt gcacggcctg cagtgtcgga cgcgtgagca tgccgcgcct
     6421 ggagcgcggc tggcgcgccc cggccgcctc atagtaggaa ttgaacagat agttgaagtg
     6481 ctcgtcgaac acgcggtagc cggcagcatg aggcttgaga accatggtct cgaaaaacca
     6541 ggtcgtgtgt gctaggtgcc atttcgccgg gctcgcgtcc ggcatcgact gcacggtggc
     6601 atccgcatcc gacagcggcg ccgacaatgc agaggtcacc tgtcgcacac cgcgatagag
     6661 cagcgtcagt tcgcttaagc ccaacgaaga gctgaacagg ggcgcggcac tcatcgcaaa
     6721 gtaaccctca gtggggtgcc gactctcgcc gccgtcgctg caccagaaag caggcgcccg
     6781 ccgcaatcgc cagtgcgagg acgctgacgt agaactggct ctgggtatcc ggcgtcacgg
     6841 ccatcgccac cagcaccgcg atcatcccgg cgatggccgc gtaactggcc cagggaaacc
     6901 accacatctg cagcgcgggt tccggctggc ccgcgcgctg ccgctgccga cgcagtcgga
     6961 tctgcgccag gcagatcaga acgtagacga acacgattag cgcgccggcc gcgttgatca
     7021 ggaacgcgaa tacttgtttg ggcgcgaacg cgttggcgag aatgcccagc acgcccgcga
     7081 tggttccaat cagcaccgaa tt
//
LOCUS       DATMRM010000017         2024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2176581, whole genome shotgun sequence.
ACCESSION   DATMRM010000017 DATMRM010000000
VERSION     DATMRM010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2176581"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            550..1254
                     /locus_tag="VNZ06_00530"
     CDS             550..1254
                     /locus_tag="VNZ06_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWW19262.1"
                     /translation="MRRCLIIEDDADNARYIADGLRELGWFVSLARNGTDGVERATSA
                     TWDLIVLDRMLPNEVDGLAILKTLRGLGQSTPVLILSALSATDERVRGLQAGCDDYLT
                     KPFAFSELAARVEALVRRSQFGKDVREMRVADMRVDLIARRVERSGRTIPLQPREFRL
                     LAYLLFHQGRVVTRTMLLEAVWDYRFDPQTNVIDVHISRLRNKIDRDAAVPLIHTVRG
                     VGYMLSADGLAPQTSA"
     gene            1251..>2024
                     /locus_tag="VNZ06_00535"
     CDS             1251..>2024
                     /locus_tag="VNZ06_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWW19263.1"
                     /translation="MTIVVPRWWTSIAFRQALIYAALVVLTMTVLLAVFYQQTVGVWQ
                     QRIDHQIDATTHRLVDYSEHKGAAALATHITALLGDGIDSDTEVYTLVDPAGHKVVGN
                     LALEPDILAPTRDIVEHRVVRAGRSSQSRLRITHLGDGGVLVVGRDMQDQRELDRLVR
                     RASLLSALVTLLMAVAGALFLRRQLEQRISAIRQTAVRIERGDLAQRVPVSQQQDEFA
                     RLSRDINAMLDRIHALMEGVRHVSNTIAHNIRTPLSRILA"
BASE COUNT          353 a          634 c          617 g          420 t
ORIGIN      
        1 aggctattcc aatcgccgtt ccatcgtgtg tcgcttcctt gcgcccgtca ttacgtgaca
       61 cggctcaaaa caagagagcc agcatgtttg ccgccaaagt gtggtgtgcc aagtggtgcg
      121 ctttcaatac gtatcactgc gtatttgcca cgcgttcggt accgcatcct ccagggaact
      181 gggagtacca ccttttttga gttggggggt ttcggccagc cggcgcggca agtaatgggc
      241 gacgacgcat cccatgaggg gccgagggcg cggggtaatg gcgacggaac ccatcgcgcc
      301 gccggccacg ctaccgcgcc ctgcaagctc tacgaccctc gcttcgactt caatgatgca
      361 gtgatcccgc tcgatagcac gatgtggatg cggctcgccg agggctggct gaacgtcgcc
      421 tcgcccgcaa cccgcaatcg ggctagtggc tgacttccgc gcagggccgc cactgctttc
      481 caggcgtctc ttcgggcgat acattgtcac cgtgccgctt cgggccggtg agcttccctt
      541 taaaaacgta tgcgccgctg tctgattatt gaggatgacg ctgataacgc gcgctacatc
      601 gccgatggtt tgcgcgaact tggctggttc gtgtccttgg cgcgcaacgg caccgatggc
      661 gtcgagcggg ctacgagcgc aacttgggac ctgatcgtcc tcgatagaat gctgcccaat
      721 gaggtcgatg gcctggccat cttgaagaca ctgcgtggcc tagggcaaag cacgccggtg
      781 cttatactca gcgcactctc ggctaccgac gaacgggttc gcggcttgca ggccgggtgc
      841 gatgactacc tcaccaagcc cttcgcgttc tccgagctcg ccgcccgcgt cgaagcgctg
      901 gtgcgacgct cccagtttgg caaggatgtg cgcgaaatgc gcgtcgccga tatgagggtc
      961 gacctcattg cgcgcagagt ggagcgatcg ggcaggacta ttccgctcca gcctcgcgag
     1021 ttccgtctgc tcgcctacct cctcttccat caaggccgtg tcgtgacacg tactatgctg
     1081 ctggaagccg tttgggatta tcgcttcgat ccgcagacca acgtgatcga tgtgcacatc
     1141 agccgcctgc gcaacaagat cgaccgcgat gccgcagtgc ccctcatcca taccgtgcgg
     1201 ggagtcggct atatgctgtc ggccgacggg cttgcacctc agacgtccgc gtgacgatcg
     1261 tggtgccgcg ctggtggact tcaattgcct tccgccaggc gttgatctat gccgcgttgg
     1321 tagttctgac catgaccgtg ctgctcgcgg ttttctatca gcagaccgtc ggcgtgtggc
     1381 aacaacgcat tgatcaccaa atcgacgcca ctacgcaccg gttggttgac tactccgagc
     1441 acaaaggtgc cgcggcgctc gcgacccaca tcaccgcact gctcggcgac ggcatcgatt
     1501 cagacaccga ggtttatacc ctcgtcgatc ccgccggtca caaagtggtg ggcaacctcg
     1561 cgctggagcc ggacatcctg gcgcccaccc gcgacatcgt cgagcatcgg gtggttcgcg
     1621 ccggacgcag ttcccagagt cgactgcgca ttacgcatct gggtgatggt ggggtacttg
     1681 tggtcgggcg cgacatgcag gatcagcgcg agctcgatcg cctggtgcgt cgtgcgagcc
     1741 tgctgagcgc gctggtcacg ttgctgatgg cggtggctgg agcgctgttc ctgcgtcgcc
     1801 agctcgagca gcgcatcagt gctattcggc aaacggctgt gcgcatcgag cgtggcgatc
     1861 ttgcgcagcg cgtgccggtt tcgcagcagc aagacgaatt cgcgcgcttg agtcgcgata
     1921 tcaatgccat gctagaccgc atccatgctc tcatggaggg cgtacgccac gtctccaaca
     1981 cgattgccca taacattcgc acaccgctgt cgcggattct ggca
//
LOCUS       DATMRM010000018         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3106440, whole genome shotgun sequence.
ACCESSION   DATMRM010000018 DATMRM010000000
VERSION     DATMRM010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3106440"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..747)
                     /gene="gph"
                     /locus_tag="VNZ06_00540"
     CDS             complement(13..747)
                     /gene="gph"
                     /locus_tag="VNZ06_00540"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01449.2"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HWW19264.1"
                     /translation="MSPAPADHLSVRSLRAVLFDLDGTLLDTVADISLALNRALAEQK
                     LASLPAAEVRVLIGRGVPTLIERALARLGAAGESSAAAQLVERFHFHYERIHELGEIQ
                     TRVYPGVTAGLAQLHALGLGLAVVTNKPNKTAVDLLTRLGLSQWIDVVVGGDSGAHRK
                     PHPQPLLTACEELAVRPAQALMVGDSLTDVLAARAAGLAVVCVPYGYNEGVDPRALPC
                     DAFVESVGALPALLTAADSRRGATAW"
     gene            complement(843..1718)
                     /locus_tag="VNZ06_00545"
     CDS             complement(843..1718)
                     /locus_tag="VNZ06_00545"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HWW19265.1"
                     /translation="MTIQHPIIAVTGSSGAGTTTVMKSFAHIFRREGIKAQVIEGDSF
                     HRYNRVEMRERVQAADRGSGPQVSHFGPEANLLRELAETFAEYGAAGRGKVRRYVHDA
                     PESKELGCDPGTFTDWQPMTSDSDLLFYEGLHGAYVGDGIDVARHVDLLVGVVPIVNL
                     EWIQKLHRDRHHRGYSQDAVVDTILRRMPDYVNHICPQFSRTHVNFQRVPTVDTSNPF
                     IARDIPSADESMVVIRFANPKGIDFPYLVSMLHNSWMSRPNNIVVPGGQMGLAMQFIF
                     TPMILRLIDLKRRAQ"
     gene            complement(1715..>2230)
                     /locus_tag="VNZ06_00550"
     CDS             complement(1715..>2230)
                     /locus_tag="VNZ06_00550"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF040139.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HWW19266.1"
                     /translation="FILTHPDLQIAADTSEFAINTSNARFWEPPVHRYVTECQAGKSG
                     IRGRDFNMRWIASLVAEVHRILMRGGVFMYPKDSKDKSKPGRLRLLYEANPISLLVEQ
                     AGGRATTGAQRLMEVKPESLHQRVPLILGSRNEVERIERYHAEYESGADRPFSSPLFN
                     ERSLFRPEALV"
BASE COUNT          394 a          729 c          720 g          387 t
ORIGIN      
        1 aggcgagcct gatcaccacg cggttgcgcc gcggcgggag tctgccgcag tcagaagtgc
       61 gggcagagcg cccacgctct ccacaaacgc gtcgcacggc agcgcacgtg ggtcgactcc
      121 ctcgttgtag ccatagggca cgcacactac ggccagcccc gcggcgcgcg cggcgagcac
      181 atcggtcaac gagtcaccca ccatcagcgc ctgagccggc cgaaccgcca gttcctcaca
      241 ggcggtcagc aggggttgcg gatgcggctt gcgatgcgcg ccgctgtccc cacccaccac
      301 cacgtcaatc cattgactca accctaagcg agtcagcagg tcaacggcag tcttgttcgg
      361 cttattggtc accactgcaa ggcccaggcc gagcgcatgc agctgagcga gccccgcggt
      421 cactcccggg tacacgcgcg tctggatttc accgagttcg tgaatccgct cgtaatgaaa
      481 gtggaatcgc tccaccagct gcgccgcagc ggatgattct ccggcagctc cgagccgcgc
      541 aagggcgcgc tctataagag tgggcacacc ccgtccgatg agcacgcgga cctcagccgc
      601 aggcaggctc gcgagtttct gctcggcgag tgcgcggttc aatgcgaggc tgatgtcggc
      661 aacggtatcc agcagcgtgc cgtccaggtc gaacagcacc gcccgcaggg accgcaccga
      721 cagatgatcc gccggggccg ggctcatgac cgcctcgatc gccagaaccg gcagcgcacg
      781 cggcagcagc gtcgcgcgat agccggcaca ggtctctctt gagcattcat gcggcagcct
      841 cgctactgtg cgcgtctctt gagatcgatc aggcgcagga tcatgggcgt gaagatgaac
      901 tgcatcgcga gccccatctg accccccggc accacgatgt tgttcggtcg gctcatccac
      961 gagttgtgca gcatggaaac cagatacgga aagtcgatgc ccttggggtt ggcgaagcgg
     1021 atcaccacca tggactcatc cgcgctgggt atgtccctgg cgatgaacgg gttactggtg
     1081 tccaccgtcg gcacccgctg gaagttgacg tgggtgcggc tgaactgcgg acagatatgg
     1141 ttcacgtagt cgggcatgcg ccgcaggatg gtgtcgacta ccgcgtcctg gctgtagccg
     1201 cgatgatgcc ggtcgcgatg cagcttctgg atccactcga gattgacgat cggcacgacg
     1261 ccgacgagca gatcgacgtg gcgtgcgaca tcgatgccat cgccgacgta agcgccgtgc
     1321 aggccttcat agaagagcag gtcgctgtcg gacgtcatcg gctgccagtc ggtgaaagtg
     1381 cccgggtcgc agccgagttc cttggactcg ggcgcgtcat gaacgtagcg acggaccttg
     1441 ccgcgaccgg ccgcgccata ctcggcgaag gtctcggcca gctcgcgcag gagattggct
     1501 tccggcccga agtggctcac ttgcggaccg ctgccgcggt cggcagcctg cacgcgctcg
     1561 cgcatctcga cgcggttgta gcggtgaaag gaatcgccct cgatgacctg cgccttgatg
     1621 ccttcgcgcc ggaagatgtg cgcgaagctc ttcatgacgg tggtggttcc cgcgccggag
     1681 gatccggtca cggcgattat cggatgctgg atggtcaaac cagcgcctcc ggccggaaca
     1741 gcgagcgttc gttgaagagc ggtgagctga acggccggtc tgcgccgctt tcgtattcgg
     1801 cgtggtagcg ctcgatccgt tcgacctcgt tgcgcgaacc caggatcagc ggaacgcgct
     1861 gatgcagcga ctccggcttc acctccatga ggcgctgggc accggtcgtc gcgcggcccc
     1921 ccgcctgctc caccagcaga ctgatcggat tcgcttcgta cagcaggcgc agccggcccg
     1981 gcttgctctt gtccttgctg tccttcggat acatgaacac cccgccgcgc atgaggatgc
     2041 ggtgcacctc cgccaccagc gaggcgatcc agcgcatgtt gaaatcgcga ccgcgaatgc
     2101 ccgactttcc cgcctggcac tccgtcacgt agcggtgcac cggcggctcc cagaagcggg
     2161 cgttgctggt gttgatggca aactcgctgg tgtcagccgc aatctgcagg tccggatgtg
     2221 tcaggatgaa
//
LOCUS       DATMRM010000019         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3420384, whole genome shotgun sequence.
ACCESSION   DATMRM010000019 DATMRM010000000
VERSION     DATMRM010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3420384"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VNZ06_00555"
     CDS             complement(<1..524)
                     /locus_tag="VNZ06_00555"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HWW19267.1"
                     /translation="MTLLLLVGLATVPAARAAGPLAGELEIRSAFVVVDHGVLQLSAH
                     IQYPINDRIRAALQEGVTLAFDLDVTISRHRRLWFNAGELEMTLRRELTYHAVTDRYV
                     VRDEKSVEQESFSTLEAALDKLGHVEDLPIIVQSQLHDNGPWEVAVRAGMRRGRMPDA
                     LRALLFWTDDWHRT"
     gene            complement(588..1898)
                     /gene="rsmB"
                     /locus_tag="VNZ06_00560"
     CDS             complement(588..1898)
                     /gene="rsmB"
                     /locus_tag="VNZ06_00560"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HWW19268.1"
                     /translation="MNEIAAAVLAEAARALGKVAFAGRSADQALDSATRAPAAVRAIT
                     LGTLRWYWRLDAVATVLLGATRVTPPLRALLLVSLHQLEYSRNPPQATVSSAVDAVRM
                     LGQPRAAGLANALLRRFLRERESLTSRALLDEAAASAHPRWLYDELRLRWPEHWRQIV
                     EANNSHPPMTLRVNLSRESRDGYQARLKQLGMEARALGWSPTALILDEPVAVNALPGF
                     GQGSVSVQDAGAQLAGALLGARPGERVLDACAAPGGKTGAILESVAGAVELTAIDVDA
                     TRVQHIAENLRRIGVRARVITADLIESATGWWDGEPFDRILLDAPCTATGVIRRHPDI
                     KLLRRAQDVEAYAGIQQRMLIGCLSLLKPGGRLIYSTCSLMPMENASVVRHALAQVPR
                     AQARPLPPAVALPPHSLSGATSVQLLPGNAALTDGFYYACLTVT"
     gene            complement(1895..>2625)
                     /gene="fmt"
                     /locus_tag="VNZ06_00565"
     CDS             complement(1895..>2625)
                     /gene="fmt"
                     /locus_tag="VNZ06_00565"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWW19269.1"
                     /translation="EPDRAAFVSWRPDVLVVVAFGLILPASVLAVPRLGCINVHASLL
                     PRWRGAAPIQRAILAGDSRTGVTIMQMAVGLDTGPILMQESIDLFGRETSLQLHQRLA
                     DLGARLLIETLDSLEACTVSLKDQSTVGVSYASKIDKHEASIDWQHSAQQIDRQVRAF
                     NPWPVAQTLWDGQQLRLWEARSVEDAAPAASPGQILGLEQDRLAVQCGQGKLEIVRLQ
                     LAGRRILSGREFSSGRILTGMRLG"
BASE COUNT          491 a          884 c          814 g          436 t
ORIGIN      
        1 ctggtgcggt gccagtcgtc ggtccagaag agcaacgcgc gcaaagcgtc cggcatgcga
       61 ccacgacgca tacccgcccg aacggcaacc tcccacgggc cattgtcgtg cagttgcgat
      121 tgcacgatga tcggcaagtc ctccacgtgt cccaacttgt cgagcgctgc ttccagcgtc
      181 gaaaaactct cctgctccac actcttttcg tcgcgcacga cgtagcgatc tgtcaccgcg
      241 tgataggtta gttcccgccg taacgtcatt tcgagctcgc cggcattgaa ccacaagcgc
      301 cggtgtcgac tgatggtaac gtccaaatcg aaagcaagcg tcaccccttc ctgcagcgcg
      361 gcgcgaatgc gatcattgat cggatactgg atgtgcgcac tgagctgaag cacgccgtga
      421 tcgaccacga caaatgcgga tcgaatctcc agttcaccgg ccaggggacc ggccgcccgc
      481 gcagccggaa ctgttgccag gccgacaagc agcagtaagg tcatacccaa tctgaggctt
      541 gtacgcacgg gttcctaaac ggcgcagcgg ccgcggttcg cgccgcccta agtcactgtc
      601 agacaagcat aatagaagcc gtcagtcagc gccgcattcc ccggcagcag ctgcactgag
      661 gtcgcgccgg agagcgagtg tggcggcaag gcgaccgctg ggggcagggg tcgcgcctgg
      721 gcccgtggca cctgcgccag cgcgtgccgg accacggacg cattttccat cggcatcaac
      781 gagcaggtcg aatagatcaa ccggcccccg ggcttgagca gtgacagaca gccaatgagc
      841 atgcgttgct ggatgcctgc gtacgcctca acgtcctgcg cccgtcgcaa caacttgatg
      901 tccggatgcc gacggatcac cccggtcgcg gtacacggtg cgtcgagcag gatgcgatcg
      961 aacggctctc catcccacca gcccgtggcc gactcgatca agtcggcggt gatcacgcgc
     1021 gcgcgtacac cgatgcgcct gaggttctcc gcgatgtgct gtacgcgtgt tgcgtcgaca
     1081 tcgatcgccg tcaactctac agcgccggcg acactctcca gaatcgctcc ggtcttgccg
     1141 ccgggcgcag cgcaggcatc aagtacgcgc tctccgggcc gcgcccccag cagcgcaccc
     1201 gcgagctgcg cgccagcgtc ctgcacggag accgaccctt gtccgaatcc cggcaacgca
     1261 ttcaccgcaa cgggctcatc gagtatcagt gcggtcggcg accagcccag cgctctggcc
     1321 tccattccga gctgcttgag ccgcgcctga tagccatcgc gcgattcacg ggacaagttc
     1381 acccgcagcg tcataggcgg atgggagttg ttggcctcca cgatctgccg ccagtgttcg
     1441 ggccagcgca ggcgcagctc gtcgtagagc catcgcggat gcgcgctggc ggccgcctca
     1501 tccaacagtg cacgggaagt caatgattcg cgttcgcgca agaatcggcg cagcagcgca
     1561 ttggcgagcc cggccgcgcg cggttgtccg agcatgcgca cggcgtccac ggccgacgag
     1621 accgtggctt ggggtggatt gcgcgaatac tccagttgat gcagcgacac cagaagcagc
     1681 gcccgtagcg gcggcgtcac ccgcgttgcc ccgagcagca ccgtcgcgac ggcatccagc
     1741 cgccaatacc agcgcagagt gccgagggtg atcgcacgca ccgccgcagg ggcccgggtt
     1801 gcgctgtcca gggcttgatc ggcagagcgt ccggcgaatg ctaccttgcc gagcgcgcga
     1861 gcggcttcgg cgagaacggc tgccgcaatc tcgttcaccc gaggcgcatc cccgtcagga
     1921 ttcgtccact gctgaattcg cgccccgaca ggatgcgtcg gccggccaat tgcaggcgca
     1981 caatctcaag tttgccctga ccgcattgga cggcgagacg atcctgctcg aggcccagga
     2041 tttgaccggg cgatgcagcc ggagcagcgt cctcgaccga gcgtgcctcc cacaggcgca
     2101 gctgctgtcc gtcccacagc gtctgagcca ccggccaggg attgaaggcc cggacctggc
     2161 gatcgatctg ctgcgcactg tgctgccaat cgatgctggc ttcgtgtttg tcgatcttgg
     2221 acgcatagct cactccgacg gtcgattggt cctttagaga cacagtacag gcctcaaggc
     2281 tatctagtgt ttcaataaga agcctcgctc cgagatcggc caggcgttga tgcagttgca
     2341 gcgaagtctc ccgcccgaac agatcgatgc tctcctgcat caggatgggt ccggtatcca
     2401 gccccaccgc catttgcatg atggtgaccc ccgtccggga gtctccggcc agaatcgcac
     2461 gttggatcgg ggcggcgccg cgccatcgcg gcagcaggga ggcgtggaca ttgatgcagc
     2521 ccagtcgcgg aaccgccagc actgaagctg ggaggatcaa cccgaaggcg accaccacca
     2581 gcacatccgg gcgccatgag acgaacgcgg ctcggtccgg ctctg
//
LOCUS       DATMRM010000020         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_320104, whole genome shotgun sequence.
ACCESSION   DATMRM010000020 DATMRM010000000
VERSION     DATMRM010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320104"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            472..846
                     /locus_tag="VNZ06_00570"
     CDS             472..846
                     /locus_tag="VNZ06_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWW19270.1"
                     /translation="MNTKQGLSPGRQRRRYSLEFKAKLVEECRRAGVSLASIALANGL
                     NANLLRRWVVEHEALAAPAPQPPTAAPNADFVALPLIAGPPAPPTADIRIEVRRGSAT
                     VTVSWPAQAAGDCAAWLREWLR"
     gene            843..1178
                     /gene="tnpB"
                     /locus_tag="VNZ06_00575"
     CDS             843..1178
                     /gene="tnpB"
                     /locus_tag="VNZ06_00575"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HWW19271.1"
                     /translation="MIRVDSVWLAVAPIDMRTGTDTALARVVAVFGSARPHNAYAFTN
                     KRANRIKVLVHDGVGIWLCARRLHQGRFVWPRDETSIRMTLTRSQFDALVIGLPWQRI
                     GAESVIALV"
     gene            1258..>2137
                     /locus_tag="VNZ06_00580"
     CDS             1258..>2137
                     /locus_tag="VNZ06_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006484960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HWW19272.1"
                     /translation="MNAAELENLDAAQLRNLTRELIEQVTEQRDELRFKTARIEQLTF
                     ELARYKRIRFEASSEKLDAAQVSLLQETLDADLAAIEEQLEELTTEPGKPRKQTPRRE
                     ALPANLPRVSIHHEPETKTCQCGCELKRIGEDVSEKLFYKPGDFTVERHVRGKWVCAK
                     CQTLIQAPVPPQVIDKGIATASLLAQVMVAKFHDHLPLYRQEGIFGRAGYAIPRSTLG
                     AWVGACGVQLQPLVDALKDAILRHDVLHADETPVAMLKPGNGKTHRAYLWTYCPGAFE
                     SMKAVVYDFTETRSGKN"
BASE COUNT          429 a          627 c          682 g          399 t
ORIGIN      
        1 gctcccctta ccttcgctct gcttgctttc ttctaatcac gttcgccatg agccaattcg
       61 cgagaatcga gttcttcacg tctcaagaat gtgttactaa gcaggggcaa ttcattgata
      121 ttcaacggca ggaacgtgag attcatgttg ctcagggcgc cgaatgcggc taatagcgac
      181 ggaagctgtc ggccagaagc gaactcaatt tcaacgcagg gcaggcctac gagacttgtc
      241 gggcgcatga ggtctgtcgc tcatttcgtt tcgatctgcg tgctgctagc gtcaaatgga
      301 tgtaccaccc tcagcgggcc tggcacgcag ccggcttcaa acgcgaccta tccccgtagc
      361 tcccgatcgt ttacatccgt ggagtatcaa ggtaagcagc cagctaaggc atcttgctcg
      421 ggccgcacct gaaggctgac gatcatgtcc gcttaggaaa taggtggaca cgtgaacact
      481 aagcaaggac tgtcgccggg ccggcagcgc aggcgctaca gcctcgagtt caaggcgaaa
      541 ctcgtcgaag agtgccgacg ggccggcgtg tcgctggcat caatcgcgct ggccaacggg
      601 ctgaacgcga acctgctacg gcgttgggtg gtggagcacg aggctctggc agcaccggct
      661 ccacagcctc cgacggcagc tcccaatgca gatttcgtgg cactgccatt gatcgctgga
      721 ccgccggcac cgcccaccgc tgatatccgc atcgaagtgc gtcgtggatc ggcgacggtg
      781 acggtcagct ggccggcaca ggccgccggc gactgtgccg cctggctgcg cgagtggcta
      841 cggtgatccg tgttgattcg gtgtggttag ccgtggcgcc gatcgacatg cgcaccggca
      901 cagatacggc gctggccaga gtggtggcgg tgttcggatc tgcgcgtccg cacaatgcct
      961 acgcgttcac gaacaagcgc gccaatcgga tcaaagtcct ggtgcacgat ggcgttggca
     1021 tctggttgtg cgcgcgccga ctgcatcaag gccgcttcgt atggccaagg gacgaaacgt
     1081 cgattcgcat gacgctgacc cgttcacagt tcgacgcact ggtgattgga ctgccgtggc
     1141 agcgcatcgg ggctgagagc gtgatcgctc tcgtgtgagt cgactgcatc gacgattcgc
     1201 ttgccgacaa gcggcgatag cgatcttgcg gcagttctgt caccatgcgt gctcatggtg
     1261 aatgccgcag aactcgagaa cctggatgcc gcgcaactgc gcaatctgac tcgggagctg
     1321 atcgagcaag tcactgagca gcgtgacgag ctgcggttca agacggcgcg catcgagcag
     1381 ctcaccttcg agctggcgcg ctacaagcgg atccggttcg aggccagcag cgagaagctg
     1441 gatgctgcgc aggtcagctt gctgcaagag acgctcgatg cggacctggc ggcgatcgaa
     1501 gagcagctcg aggaactgac caccgaaccg ggcaagccgc gcaagcagac gccgcgtcgg
     1561 gaagccctgc cagcgaacct tccgcgcgta tcgatccatc acgagccgga aaccaagacc
     1621 tgccagtgcg gttgcgaact gaaacgtatt ggcgaggacg tctctgagaa gctgttctac
     1681 aaaccgggcg acttcactgt agagcggcac gtccggggca agtgggtgtg tgccaaatgc
     1741 cagactctga tccaggcgcc ggtgccgccg caggtgattg ataaaggcat cgccactgcg
     1801 agtttgttgg cgcaggtgat ggtggccaaa ttccatgatc atttaccgct gtaccgccag
     1861 gagggcatct tcggccgagc tggctacgca attccacgct ccacgctcgg cgcgtgggtc
     1921 ggcgcctgcg gagtgcagct gcagccgctg gtggatgccc tgaaggacgc gattctgagg
     1981 cacgatgtgc tgcacgctga cgagacgccc gtggcaatgc tgaagcccgg caatggcaag
     2041 acgcaccggg cgtacctctg gacctactgc ccgggcgcct tcgaatcgat gaaggcggtc
     2101 gtgtacgact tcacggagac cagatcgggc aagaacg
//
LOCUS       DATMRM010000021         4038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3108330, whole genome shotgun sequence.
ACCESSION   DATMRM010000021 DATMRM010000000
VERSION     DATMRM010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3108330"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /locus_tag="VNZ06_00585"
     CDS             complement(<1..1663)
                     /locus_tag="VNZ06_00585"
                     /inference="COORDINATES: protein motif:HMM:NF045328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19273.1"
                     /translation="MPDVLSSPSGMRAEFNASGSLCRLNCGAVSLSLFIGNEIEGGPA
                     NLYLRRLGKSIEWTPLLGPHSRTCFAKVGSGDALIGDGEWLGMRYRVALHFAQQSPAW
                     FWHVRLENTSTDAQEVDLTYAQDLALAPYGAVRMNEFYVSQYIDHTPLTDPKRGVMIA
                     SRQNAAADGRNPWSLIGSLRSGSSYATDALQFYRLSARIGAAPVALLRDLPGSRLQHE
                     HSMVVVRDVPIHLEPRATAEAGFFGCFVADHPAASSLSDLQHVSATLTLPEAAPPMRN
                     IGAVSAASSISAASLFDSAPLMNGEELDVPALQEFFGSQWRHEELTVRAELGSFFCGT
                     NRHVVLQAKELEVLRPHGHLLRSGNHLTPDEAALTSTVWMNGVFNSMLTQGHVSINRM
                     LSTTHSYLGLFRSHGQRVFVEVGGRWHLLHVPSVFEISPTACRWVYRHAKGVVEVRTA
                     ALGSPHELVLSIDVRSGGATRFLISHHCALNGDDGNLPGAVSWRQDPEGISLLPGSGS
                     EVAHRFPDGSFQILPQPGTRLSQVGGDELLYADGKPRQQPFVVIVT"
     gene            complement(1922..2845)
                     /locus_tag="VNZ06_00590"
     CDS             complement(1922..2845)
                     /locus_tag="VNZ06_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWW19274.1"
                     /translation="MRATAVFYVLVALFALAVPLLGIYPVFMMKLLCFAMFACAYNLL
                     LGFSGMLSFGHAAFFGASAYATAWLATAKGWGPAASILAGVAVSVVLGAVIGAIAIRR
                     RGIYFAMITLALGQLVYFVCLEAPFTGGENGLQSVPRGNFLGVLPLQSDTGMYYLVLG
                     AFLLVFLFIRRVVHSPFGQVLKAIRENEPRALSLGYDIDRYKLLAFILSAALAGLAGS
                     LKALILSFVTLSDVLQANSGEVILMTLVGGTGTFFGPVVGAGVIVTLQEYLSDFFGGW
                     VSVIIGAVFIACVLTFRRGIVGELVRLRANR"
     gene            complement(2849..3733)
                     /locus_tag="VNZ06_00595"
     CDS             complement(2849..3733)
                     /locus_tag="VNZ06_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWW19275.1"
                     /translation="MTEVFGIPVAVILGQLMLGLVNGAYYAMLSLGLAVIFGLMGIVN
                     FAHGAFYALGAFAALLMLQYWQVNYWFALLLAPLLIGLIGIVIERLFLRHLYKLDPLY
                     GLLLTFGLALVVQGLLHQLIGASGQTYPVPSSLAGALNLGFMILPIYRAWVILVSIVV
                     CFSTWFVIERTRLGATLRAARENAALLQAFGVNVPVLVVATFGAGVALAALAGVLSAP
                     IILVNSEMGSSLVVVVFAVVVIGGMGSILGSIVSGLILGMVEGLTKVLYPEASNVIVF
                     VIMALVLILRPAGLFGKE"
     gene            complement(3768..>4038)
                     /locus_tag="VNZ06_00600"
     CDS             complement(3768..>4038)
                     /locus_tag="VNZ06_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWW19276.1"
                     /translation="YLKAVKAVGGSDPDKVMAELKKTRINDMFAKNGYIRADGVMVHD
                     MYVMQVKTPKESKYPWDYYKVVKTMPGEEAFGPITGACSFAPAKK"
BASE COUNT          926 a         1296 c         1148 g          668 t
ORIGIN      
        1 ccgtcacgat cacgacgaac ggttgctgcc gcggctttcc gtccgcatac agcagttcat
       61 cgcctcccac ctgcgagagc cgggtgcccg gttgcggcag gatttggaaa ctgccgtcag
      121 ggaagcgatg cgcgacctcg ctacccgacc cgggcaagag cgagatgccc tccggatcct
      181 gacgccagct tactgccccg ggcagattgc cgtcgtcacc gttcagcgcg caatggtgtg
      241 agatcagaaa tcgggtcgcg ccgcctgaac gcacgtcaat tgacagcacc agttcgtgtg
      301 gactgccgag cgcggcagtg cgtacctcga caacaccttt ggcgtgccga tacacccagc
      361 gacaggctgt gggtgatatc tcaaataccg acggcacgtg caacagatgc catcggccgc
      421 cgacctcgac gaacacgcgc tgaccgtgtg agcgaaacaa ccccagataa ctatgagtcg
      481 tggacagcat ccggttgatg ctcacgtgtc cctgcgtcag catcgaattg aacacgccat
      541 tcatccagac ggtcgacgtc aacgccgctt catccggagt caggtggttg ccgctgcgca
      601 gtagatggcc gtgcgggcgc agtacttcga gttccttcgc ctgcagcacc acgtgccggt
      661 tggtgccaca gaagaacgac ccgagctcgg ctcgcactgt cagttcctcg tgtcgccact
      721 gcgagccgaa aaactcctgc agggctggca catccaactc ttcgccgttc atcagtggcg
      781 cactgtcaaa cagcgatgcg gcgctgattg acgacgccgc tgagaccgcg ccgatgttac
      841 gcatcggcgg tgcggcttcc ggcagggtaa gcgtcgcgct gacgtgctgc agatcgctga
      901 gcgagctggc cgctgggtga tcggccacaa aacaaccgaa gaagccagcc tcggctgtcg
      961 cgcggggttc cagatgtatc ggcacatcgc gcacgacaac catggaatgt tcatgctgca
     1021 gacgcgatcc tggcagatca cgcagcaacg ccaccggtgc cgcaccgatt cgcgctgaca
     1081 gccgatagaa ctgcaacgcg tcagtcgcgt acgaactacc acttcgcagc gagccaatca
     1141 ggctccaagg attgcgacca tcggccgcgg cattctgtcg cgaggcgatc atcactccgc
     1201 gcttcggatc ggtaagcggt gtgtggtcaa tgtattggct gacatagaac tcgttcatgc
     1261 gcacggcacc ataaggcgcc aatgccagat cctgcgcata cgtgagatcg acttcctgag
     1321 cgtcggtgct ggtattttcc agtcgcacat gccagaacca cgccggcgac tgctgcgcaa
     1381 aatgaagcgc tacccggtag cgcatgccga gccattcccc gtccccgatc aaagcatcgc
     1441 cagaccccac cttggcaaag caagtccggc tgtgcggtcc gagcaagggg gtccattcga
     1501 tgctcttgcc gaggcgacgc agatacagat tcgcgggtcc cccctcgatc tcgttgccga
     1561 tgaacaatga cagcgagact gcaccgcaat tgaggcgaca caatgaaccg ctggcattga
     1621 actcagcacg cattccggag ggactactga gaacatctgg catgatgtgg gctcgggcgc
     1681 ttgccggatc cacgcaactt acctcgcccg aacggctcgc acaacctcga aacccaacga
     1741 aagggaacgg gtcatgatcg gatacgtcac gctcggaacc gacgatctgc aacgcgccgt
     1801 ggctttctac aacgccctgc tcgaggagat cggcgctaaa cgaatgatgg cggatgagcg
     1861 ctttgtggcc taacttcttt cacatgggct gaaccgcatc gcgaggatca acgccgcagg
     1921 cttagcggtt cgcgcgcagc cgcaccagtt cgccgacgat gcctcgtcgg aaggtcagta
     1981 cgcaggcaat gaacaccgcg ccgatgatca cgctgaccca gccaccgaaa aagtcggaga
     2041 gatattcctg cagcgtcaca atcaccccgg cgccgaccac cgggccaaag aatgttccgg
     2101 tacctcctac cagggtcatc aggatgactt caccggagtt ggcctggagc acatccgaga
     2161 gggtcacgaa actcaaaatc agcgctttca atgaaccagc caggccggcc agtgcagccg
     2221 acaggatgaa cgccagcagt ttgtaacgat cgatgtcgta gcccagtgac agcgctcgcg
     2281 gttcattctc gcgaatcgcc ttgagcacct gaccgaacgg cgaatgcacg acgcgccgga
     2341 tgaacaggaa cacgagcaga aatgcgccca acaccaggta atacatcccg gtatcggact
     2401 gcaggggcag tacacccaga aaattgccgc gcggcacact ttgcagcccg ttctcgccgc
     2461 cggtgaaggg cgcctcgagg cacacgaaat acaccagttg tcccaacgcc agcgtgatca
     2521 tcgcgaagta gatgccgcga cgtctaattg caatcgcgcc gatgaccgcc cccagcacca
     2581 ccgagacggc aacgcccgca agaatcgagg ccgccggacc ccatcccttc gcggtcgcga
     2641 gccacgcggt ggcataggcc gacgcgccaa agaaggctgc atggccaaac gacagcatgc
     2701 cgctgaatcc gagcagcagg ttataagcgc acgcgaacat cgcaaagcac agcagcttca
     2761 tcatgaacac cggatagatg cccaggagcg gaacggccag agcaaacagt gcgaccagga
     2821 cgtagaaaac cgctgtcgcc cgcacgctct attccttacc gaacagaccg gcgggtcgca
     2881 gaatcagcac cagcgccatg atgacgaaca cgatgacgtt ggacgcctcc ggatacagca
     2941 ccttggtcag gccctcgacc atccctaaga tcagtccgct gacgatcgag cctagaatcg
     3001 agcccatgcc accaatgacc accacggcga agaccacaac gaccagtgaa ctgcccatct
     3061 cggaattcac caggatgatc ggcgccgaca gcacgccggc cagcgcggcg agcgccacgc
     3121 cggcgccgaa tgtcgcgacc accagcaccg gtacgttcac gccgaacgcc tgcagcaacg
     3181 ctgcgttctc gcgcgccgcc cgcagggtcg cgccaagccg ggtgcgctcg atcacgaacc
     3241 aggtgctgaa gcagacgacg atggagacca gaatcaccca ggcacggtag atcggcagga
     3301 tcatgaatcc gaggttcagt gctccggcca gggatgacgg tactggatag gtctggcccg
     3361 atgcaccgat gagctgatgc agcaggccct gcacgaccag ggcgagaccg aaggtcaaca
     3421 gcaatccgta caaggggtcg agcttgtaca gatgtcttag aaaaaggcgc tcgatgacga
     3481 tgccgatcaa cccgatcaac agcggggcca gcagcagtgc aaaccaatag ttcacctgcc
     3541 agtactgcag catcagcagt gccgcgaacg cgcccagcgc atagaacgcg ccgtgcgcaa
     3601 aattgacgat gcccatcaat ccgaagatca ccgcaagacc caaactcaac atcgcgtagt
     3661 acgcgccgtt gaccagcccg agcatcagct ggcccaggat gacggcgacg ggaatgccga
     3721 aaacctcagt catgcggctt cccgcttatg ggagcaggac agatcttcta ttttttcgca
     3781 ggcgcgaagg aacaggctcc ggtaatcgga ccaaaggcct cttcgcctgg catcgtcttg
     3841 acgactttgt agtagtccca cggatacttg gactccttcg gggtcttgac ctgcatcacg
     3901 tacatgtcgt ggaccatcac gccgtccgcg cggatgtagc cgttcttggc aaacatatcg
     3961 ttgatccggg tcttcttcag ctcggccatg accttgtctg gatcgctgcc gcccacggcc
     4021 ttcacggctt tcagataa
//
LOCUS       DATMRM010000022         3869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_939778, whole genome shotgun sequence.
ACCESSION   DATMRM010000022 DATMRM010000000
VERSION     DATMRM010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939778"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VNZ06_00605"
     CDS             complement(<1..287)
                     /locus_tag="VNZ06_00605"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438191.1"
                     /note="catalyzes the formation of
                     1-(5-phosphoribosyl)-5-aminoimidazole from
                     2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in
                     purine biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HWW19277.1"
                     /translation="MSEPHSYTYRDSGVDIDAGDELVERIKPMVRRTTRPEVLAGIGG
                     FGALVEVPLGFRRPVLVSGTDGVGTKLRLAIQTGQHSTIGIDLVAMCVNDVV"
     gene            349..894
                     /locus_tag="VNZ06_00610"
     CDS             349..894
                     /locus_tag="VNZ06_00610"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWW19278.1"
                     /translation="MRHLPNLICLFRVALVWPILASITRGQYALTLLLFFIAAASDGL
                     DGYLAKRFNWTSDLGKILDPAADKLLLVSVFLVATWYGLIPRWLTIAAVARDVSIPLG
                     ALAYRVVSGPIRGQPMISSKINTLFQVLYILAVIAHAAFGFPPDTILDALAVVTLATV
                     LISGSAYVIEFTRRALQVADA"
     gene            891..1601
                     /gene="hda"
                     /locus_tag="VNZ06_00615"
     CDS             891..1601
                     /gene="hda"
                     /locus_tag="VNZ06_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HWW19279.1"
                     /translation="MSQLTLALRLRERAVFDSFVVGPNAAAVEQLQTIAAGSIAGMFW
                     LSGPSGVGKSHLLQAVCALARERETEAAYLSLAQLQQFGPEALDGWHSARIVAIDELA
                     RVAGERTWEQKLFGLYRELEERAATLIAAAADPPRLLNFALPDLASRYSAATLITLRA
                     LDETAQREALRVRAQARGLELPEETALYLQRRFPRDLPTLYELFDRIDDAAFRAQRRL
                     TVPFIREVLSRYLPGEST"
     gene            complement(1555..2175)
                     /gene="wrbA"
                     /locus_tag="VNZ06_00620"
     CDS             complement(1555..2175)
                     /gene="wrbA"
                     /locus_tag="VNZ06_00620"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HWW19280.1"
                     /translation="MAEILLVYYSRNGATAELARQACRGIESVAGASATLRTVPALTP
                     SSEAPPPQAVPENGPPYATLEDLRRADALMLGSPTRFGNMAAPLKYFIDATSALWLDG
                     SLAGKPAAVFTSTQTMHGGQETTLLSMMLPLLHHGMVLVGLPYTERALSSTRSGGTPY
                     GASHVAGVQQALTEEEKLLAQALGRRLAELAVRLTRPAGSGTAPRG"
     gene            2206..2484
                     /locus_tag="VNZ06_00625"
     CDS             2206..2484
                     /locus_tag="VNZ06_00625"
                     /inference="COORDINATES: protein motif:HMM:NF011022.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HWW19281.1"
                     /translation="MRVGRRCVVSGRVQGVFYRASARQRAQGSGVTGYAKNLSDGSVE
                     VLACGEEAAVLEFIRWLWTGPSAAKVADVKIETVEFAPDDWPPGFRTS"
     gene            complement(2471..3118)
                     /locus_tag="VNZ06_00630"
     CDS             complement(2471..3118)
                     /locus_tag="VNZ06_00630"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWW19282.1"
                     /translation="MMHWRSFELACRASCWALLRALLLTFPALLAAAAPPQQDPELRA
                     VVQQAISEAQCFADKYDSAVWFTLMEPKLRRYVRDKDERLQILQTVFCEAHRSGATLL
                     PPGLVMAVLDVESGFDRWAVSSAGAVGLMQVMPFWPERLGMHRYELTHIEPNLHMGCA
                     ILRFYLDAERRDVRKALERYNGSVGHRDYPDRVIVRWTTYWHGADDLGRGPVKTS"
     gene            complement(3078..>3869)
                     /locus_tag="VNZ06_00635"
     CDS             complement(3078..>3869)
                     /locus_tag="VNZ06_00635"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HWW19283.1"
                     /translation="KLSGVASPLRMAGLAEMRAAAGTEPGFLGPVNLKGVRIYADHAT
                     LAMADFICGANEKDAHFTGVNWGRDLPEATAADLRNVVNGDPSPSGKGVLRIMRGIEV
                     GHIFQLGCVYSQSMKATVLDEAGNPVEMLMGCYGIGVTRVVAAAIEQNHDERGIIWPD
                     AIAPFHVVLVPLNLQKSARVRSTADALYAQLTAGGIEVLYDDRDARPGVKFADAELIG
                     IPHRIVVGERGVEAGTLEYRHRRATESEDFPAHDALAFIRARLQS"
BASE COUNT          663 a         1243 c         1242 g          721 t
ORIGIN      
        1 accacatcgt tgacgcacat ggccaccaga tcgataccga tggtcgaatg ctgtcccgtc
       61 tggatcgcca aacgcagctt ggtccccacg ccgtcggtgc cggaaaccag tactggccgg
      121 cggaagccca gcggtacctc caccaaggcc ccgaatccgc cgataccggc gagcacctcc
      181 gggcgtgtgg tgcgtcggac catcggcttg atgcgctcga ccagctcatc gcccgcgtcg
      241 atgtccacgc ccgaatctcg ataggtatag ctatgtggtt cgctcatgac agggcgttta
      301 tagtagccga ggatgctaag gaaagcggga cattaagaac acgccgccgt gcgtcacctg
      361 cccaatctca tatgcctgtt tcgcgttgcg ctcgtgtggc cgattctggc ctcgatcacg
      421 cgcgggcagt atgcattgac gctgctgctg ttcttcatcg cagcggccag cgatgggtta
      481 gacggctatc tggctaagcg gttcaactgg acctctgacc ttgggaaaat actggatccg
      541 gccgccgaca agctgctgct cgtcagcgtg tttctggtcg ccacctggta cggtctgatc
      601 ccgcgctggc tgaccatcgc ggcggtcgcc agggatgtct cgattcccct cggagcactg
      661 gcgtatcgtg tcgtcagcgg gccgatccgt ggccaaccga tgattagcag taagatcaat
      721 acgctgtttc aggtgctgta tatcctggcg gtgattgctc acgccgcatt cggatttccg
      781 cccgacacca tcctcgacgc gcttgcggtc gtgacgttag ccaccgtact gatcagcggc
      841 agcgcctacg tgatcgagtt tactcgacgc gcgctgcagg tcgcggacgc ttgagtcaac
      901 tgacgctggc attgcggctg cgcgagcgcg cggtgttcga tagttttgtc gtcggcccca
      961 acgcggccgc ggtcgagcag ttacagacca tcgccgccgg ctcgatcgcc ggcatgttct
     1021 ggctgtccgg cccttcaggg gtcggtaaga gtcatctgtt gcaggcggtc tgtgcgctgg
     1081 cgcgggagcg ggaaactgaa gccgcgtacc tgtcgctggc gcagctgcaa caattcggtc
     1141 cggaggcact ggatggctgg cacagcgcgc gcatcgtagc gatcgacgag ctggcacggg
     1201 tcgctggtga gcgcacctgg gagcagaagt tgttcgggtt gtatcgcgag ctcgaggaac
     1261 gggccgcgac cctgatcgcc gccgccgccg atcctccacg gctgctgaat ttcgcgctac
     1321 ccgatctggc gtcgcgctac agcgcagcga cgctgattac attgcgggcg ctggatgaga
     1381 cagcgcagcg cgaggccctg cgcgttcggg cccaggcgcg cggcctggag ctgcccgagg
     1441 agacggcgct gtatctgcaa cgtcgctttc cacgcgatct gccgacgctg tacgagctgt
     1501 tcgatcggat cgacgatgcg gcatttcgcg cacagcgtcg tctgacggtg ccgttcatcc
     1561 gcgaggtgct gtcccgctac ctgccgggcg agtcaacctg acggcaagct cggcgagtcg
     1621 gcggcccaac gcctgtgcca gcagtttctc ctcttcggtc agcgcctgct gcacgcctgc
     1681 gacatgcgat gcgccgtacg gcgtgccacc gctgcgcgtg ctgctcaacg cgcgttcggt
     1741 atagggcagt ccgaccagaa ccatgccgtg atggagcagc ggcagcatca tcgacagcag
     1801 ggtggtttcc tgtccgccgt gcatcgtctg cgtcgaggtg aataccgcag cgggtttgcc
     1861 ggccagtgac ccgtcaagcc agagcgccga ggtggcgtca ataaagtatt tgagcggtgc
     1921 cgccatgttg ccgaagcgag tcggactgcc gagcatcagt gcatcggcac gcctcagatc
     1981 ctcgagcgtc gcgtacggcg ggccattctc gggcactgcc tgcggcggcg gggcttcgct
     2041 gctcggcgtc agcgccggta cggtgcgcag cgtcgcactg gctccggcca ccgactcgat
     2101 gccgcgacag gcctgccgtg ccagctcggc ggtggcaccg ttgcgcgagt agtagaccag
     2161 cagaatctcg gccatgcggc cagtctaatc cgtttatgat caatcatgcg tgtcggtcgg
     2221 cgctgcgtgg tctcaggcag agtgcagggc gttttctatc gcgccagtgc gcggcagcga
     2281 gcccagggca gtggtgtgac cggatacgca aagaacctat cggatggctc ggtcgaggta
     2341 ctcgcgtgtg gtgaggaagc ggcggtgctc gaattcatcc gctggctgtg gacaggtccg
     2401 tccgccgcca aagtcgccga cgtgaagatc gaaaccgtcg aattcgcacc ggacgactgg
     2461 ccgcccggat tcaggacgtc ttgaccggac cgcggcccag gtcgtcggcg ccgtgccaat
     2521 aggtggtcca gcgcacgatc acccgatccg gatagtcgcg gtgtccgacg ctgccgttgt
     2581 agcgctccag ggctttgcgg acgtcgcgcc gctcggcgtc gaggtagaag cgcaggatgg
     2641 cgcaacccat gtgcaggttc ggctcgatgt gagtcagttc gtagcggtgc atccccaggc
     2701 gttcgggcca gaacggcatg acctgcatca gaccgacggc gccggcactg gaaaccgccc
     2761 agcggtcgaa tccgctttca acgtccagca ctgccatgac cagtcccggc ggcaacagcg
     2821 tggcaccgga gcggtgtgcc tcgcaaaaca cggtctgcag tatctgcagg cgctcgtcct
     2881 tgtcgcggac gtagcgccgc agtttcggct ccatcaacgt gaaccagacc gccgagtcat
     2941 atttgtcggc gaagcattgt gcttcgctga tcgcctgctg cacgaccgcg cgtagctccg
     3001 gatcctgctg cggcggcgcg gccgcagcca gcagagctgg aaaagtgagc agcagggcgc
     3061 gcagcagcgc ccagcagcta gctctgcagg cgagctcgaa tgaacgccag tgcatcatgc
     3121 gcggggaaat cctcgctctc ggtggcgcgt cgatgacggt actccaaagt tccggcctca
     3181 accccgcgtt cgccgaccac aatgcggtgc ggtatcccga tcagctcggc atccgcaaac
     3241 ttgacgccag gtcgcgcgtc ccgatcgtcg tacagcacct caatgccccc cgcggtcaac
     3301 tgcgcataga gcgcatcggc ggtgctgcga acacgggcgg acttctgcag attgagcggc
     3361 accaggacca catggaacgg tgcgatcgcg tcgggccaga taattccgcg ctcatcgtga
     3421 ttctgctcga tcgcagccgc cacgacgcgc gtcacaccta tgccgtagca gcccatcagc
     3481 atctccaccg gattgccggc ttcatccagc acagtggcct tcatcgattg gctatagacg
     3541 cacccgagct ggaagatgtg cccgacctcg atgccgcgca tgatcctgag cacgcccttg
     3601 ccggacgggc tgggatcgcc attgacgaca ttgcgcaggt ccgccgccgt cgcttcgggc
     3661 agatcccgac cccagttaac gccggtgaaa tgggcatcct tttcattggc gccgcaaatg
     3721 aaatcggcca tcgccagcgt ggcgtggtcg gcgtagatac gcacaccctt cagatttacc
     3781 ggtccgagaa aaccaggctc ggtgcctgcg gcggcccgca tttccgccag gccggccatg
     3841 cgcagcgggc tggcgacccc ggatagttt
//
LOCUS       DATMRM010000023         4465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_939987, whole genome shotgun sequence.
ACCESSION   DATMRM010000023 DATMRM010000000
VERSION     DATMRM010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939987"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VNZ06_00640"
     CDS             complement(<1..385)
                     /locus_tag="VNZ06_00640"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HWW19284.1"
                     /translation="MPAPIALSAGEPAGIGPDLCLAAACMPRRWPLVCIADRELLHAR
                     AHQLGLGVQLSDYRPGAEVPLGGGTLCVLHQPLAARCVAGQLEVRNARAVLGWIDRGV
                     DGCLSGEFRALVTAPVQKSLIHQSGT"
     gene            complement(396..1706)
                     /locus_tag="VNZ06_00645"
     CDS             complement(396..1706)
                     /locus_tag="VNZ06_00645"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWW19285.1"
                     /translation="MRRSLLTSLACTLALSFAGGAQASKELSNKGVLLDKVVAVVNDG
                     VVLESELDEQIKEIENRLHDQRVVLPSEDVLRSQVLDRLIVEEIQAQHADRAGIKVSD
                     EQVNAAMADIAQRQNITLAQLPERLASEGIDYPQYRNELRREIARQILRQRDVVSRIV
                     VTPHELDQYLDHQKKTASAANEYNVSHILIALAQDAKPGQLASALKLAHDVDERARNG
                     EDFNKLAVTYSQSETALEGGTLGWRKGTELPTFLADAIARMQAGEVSDVIQTPSGFHI
                     IKLNETRSAGGPQIIQQLHLRHILLKPTEIEDDATVQQRLARWREQILSGKEEFAVFA
                     KTYSQDPSSAINGGDLGWEEPNTFVPEFAAVCSKLKEGEISQPFRTQYGWHIVQMLGK
                     RDFDNTNEAARERAFAAMRDSRVEEATELWLQQIRDESFVDRRL"
     gene            complement(1738..4203)
                     /gene="lptD"
                     /locus_tag="VNZ06_00650"
     CDS             complement(1738..4203)
                     /gene="lptD"
                     /locus_tag="VNZ06_00650"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HWW19286.1"
                     /translation="MIASLRLVPLVVLCCAPWMRAVAADASSEAGTESCSGGYGYLTA
                     LMPLLAAADAAPPPGLKRTFQIGDDISFTAQGATGDLLNGYSVLTGDVDVHIGDRELQ
                     ADQITRDNTNNTMNAVGQVRFRNPIVMVLGDAGRYTDDGAMFTHAQFQFQKQPGYGSA
                     DRFVWTPDNVIAVQNVVYTSCPPPKAVWRIRARQLKLDTARGEGVGRDARLEIEGIPV
                     LYTPWISFPLSDARKSGFLFPEFSTSSPNGITVATPWYWNIAPNQDATITPTIYALRG
                     VDLGVQYRHLTEIDSGTLNVNFLPDDQRQKEQDRSLVRLVDRVSLPFNSRVDVNYENV
                     SDVQYFEDFSQGTEATSTPFLPRSVAIAHRDDVWNLRAQLSGFQTLDTVNLIEYQRPF
                     LELPRVTIGGLWSAPVLPSLTLSFNSEVVNFTRSCDSILPPQQQPLPGIPCSAIVNPE
                     FPVSAAVSGWRLDAKPQIGLDLSGPGYFFRPSAAWDLTQYELRNATNTDTPQPGSPID
                     TSPQRSLPILDLDTGLQFERMTGSSEGRSVTFEPRLMYLYIPYRKQSDLPVFDTSTPD
                     LNTIELFRPNRFVGIDRIGDSNSLTVGATTQWFNNTSGARYLSATLAQAFYLQPPKVT
                     APDETLAPHTTSSLIAEVSLNNYRHWYVQVDLASSPTRNRIEQAQIMTRYLANDKQVI
                     NIGYLYRYGVLQQVDGSAAWPISRHWDIYARTVYSLFGAPPMTSIVATNFPVTTPAAH
                     PGSIEDFAGFQYRGSCWSIRAIAQHSISTRTGQSDTGVSLQVELTGLSNVGNGVGTGS
                     GVNTFLEQSIRGYSASSPYKPLN"
     gene            4266..>4465
                     /locus_tag="VNZ06_00655"
     CDS             4266..>4465
                     /locus_tag="VNZ06_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWW19287.1"
                     /translation="MTNSDERVALIRAWLTSELQLRVSRLEPASADASFRRYFRIWED
                     NGATRIVMDAPPDKEDIGPFLQV"
BASE COUNT          802 a         1341 c         1423 g          899 t
ORIGIN      
        1 cggtgccgga ctgatggatc aggctcttct gcaccggcgc ggtgaccaga gccctgaatt
       61 cgcccgacag acacccgtcc acgccgcgat cgatccagcc cagcacggcg cgcgcattcc
      121 tcacttccag ctgaccggcc acacagcgcg cggccagcgg ctgatgcagt acgcacaagg
      181 taccaccacc cagcggcact tctgcgccag gccgatagtc ggacagttgc acgccgagtc
      241 cgagctgatg cgcccgcgcg tgcagcagct cgcggtcggc gatgcatacc agtggccatc
      301 gccgtggcat gcaggccgct gccagacaca aatcgggacc gatccccgct ggctcaccgg
      361 cgctcagcgc gatcggggcg ggcatcgcgc tgcctctata aacgtctgtc tacgaaggac
      421 tcgtcgcgta tctgctgcaa ccaaagttca gtggcctctt ccacacggct gtcgcgcatc
      481 gccgcgaagg cgcgctcgcg tgcggcctca ttggtgttgt cgaagtcgcg cttgccgagc
      541 atctgtacga tatgccagcc atactgggtg cggaacggct gactgatttc cccttccttc
      601 agcttgctgc agaccgccgc gaactcgggt acgaaggtgt tgggctcttc ccaacccagg
      661 tcaccgccgt tgatggccga actcggatcc tgcgagtagg tcttcgcaaa gaccgcgaat
      721 tcctctttac cgctcagaat ctgttcacgc cagcgggcga ggcgctgctg tacggtcgcg
      781 tcgtcttcga tctcggtagg cttcagcagg atgtgtcgca ggtgcagctg ctggatgatt
      841 tgcgggccgc ccgcggagcg cgtttcgttg agtttgatga tatgaaatcc gctgggcgtc
      901 tggatgacgt cgctgacctc gcccgcctgc atgcgcgcga tcgcatcggc caggaaggtc
      961 ggcagctccg tgcctttgcg ccagccgagt gtgccgcctt ccagtgccgt ttcgctttgg
     1021 gagtaggtca ccgcgagctt gttgaagtcc tcgccgttgc gtgcgcgctc atccacgtcg
     1081 tgcgccagtt tcagcgcgct cgcgagctgt ccgggtttcg cgtcctgcgc cagcgcgatc
     1141 aggatgtgcg atacgttgta ctcgttcgct gccgaggcgg tcttcttctg atgatcgaga
     1201 tactggtcga gttcgtgcgg cgtgaccacg atgcgcgaca ccacgtcccg ctgccgcagg
     1261 atctggcgtg cgatctcgcg ccgcagttcg ttgcgatact gagggtagtc gataccttcg
     1321 gaggccagac gttccggtag ctgcgccagc gtgatgttct ggcgctgcgc gatatcggcc
     1381 atcgccgcat tcacctgctc gtcggatacc ttgatgccgg cgcggtcggc gtgttgcgcc
     1441 tggatttcct cgacaatcag ccggtccagc acctgagagc gcagcacatc ttcggacggc
     1501 agcacgacgc gctgatcatg cagccggttc tcgatctctt tgatctgctc gtcgagctcg
     1561 ctttccagta ccacgccgtc attgacgacc gcgacgacct tatcgagcag cactcctttg
     1621 ttggagagtt ccttggaggc ttgtgcgcca cccgcgaagc tcagggcgag tgtgcaggca
     1681 agcgatgtca gtaggctgcg acgcatgaat atctctttgg ccctcaaagg gttaagttca
     1741 atttaagggt ttgtagggcg aggaggccga gtatccccga atcgactgct caaggaaagt
     1801 gttgacgcca ctgccagtgc cgacaccgtt tccgacattg gacagtcccg tgagttccac
     1861 ctgcagcgac acgccggtat cgctttgacc ggtgcgagtg ctgatggagt gctgtgcgat
     1921 ggcgcgaatg ctccagcacg agccgcggta ctggaaaccg gcgaagtcct cgatcgagcc
     1981 tggatgggct gcaggcgtgg tcacggggaa attcgtcgcc acgatgctcg tcatgggggg
     2041 agcgccgaac agggaataca ccgtgcgcgc atagatatcc cagtgcctgc tgatcggcca
     2101 cgcggccgat ccgtccacct gctgtaatac accgtagcgg tagagatagc cgatattgat
     2161 cacctgtttg tcgttagcca ggtatcgcgt catgatctgg gcctgttcga tgcggttgcg
     2221 cgtcgggctg gaggccagat ccacctgtac gtaccagtgc cggtagttgt tcagactcac
     2281 ttcggcgatc aggctggagg tggtgtgcgg cgccagcgtt tcatccggcg ccgtgacctt
     2341 gggcggctgc aggtagaagg cttgcgccag ggtcgcgctc aggtagcgtg caccactggt
     2401 gttgttgaac cactgagtgg tagccccgac cgtcagcgaa ttcgagtcgc cgatgcgatc
     2461 gatgccgacg aaccggttcg gtcggaacag ctcgatcgtg ttcagatccg gtgtggaggt
     2521 atcaaagacc ggcaggtcgc tctgcttgcg gtaaggaatg tagagataca tgagccgggg
     2581 ctcgaacgtg acgcttcgac cctcgctcga gccggtcatg cgctcgaatt gcaaccccgt
     2641 atccagatcg aggatcggca ggctgcgctg gggcgaggtg tcgatcgggc tgccgggctg
     2701 tggcgtatcg gtattggtcg cgttacgcag ctcgtattgc gtcagatccc aggctgcgct
     2761 ggggcgaaag aaatacccgg gtccggacag gtccaagccg atctgcggct tggcatccag
     2821 gcgccagcca ctgaccgccg cgctgaccgg aaactccgga ttgacgatcg cagagcaggg
     2881 aatgcccggc agtggctgct gctgcggcgg caggatcgag tcgcaggagc gggtgaagtt
     2941 gaccacctcg ctgttgaacg acagggtcag cgacggcagc actggcgctg accacagccc
     3001 accgatcgtc acccggggca gttcgaggaa aggacgctgg tactcgatga gattgaccgt
     3061 atcaagtgtc tggaaccctg acaactgtgc ccgcagattc cagacgtcat cgcgatgcgc
     3121 gatcgcgacg ctgcgaggca gaaagggcgt gctggtggct tccgttccct ggctgaagtc
     3181 ctcgaagtat tgtacgtcgc tgacgttttc gtagttgacg tcgacgcggc tgttgaacgg
     3241 caggcttacc cgatcaacca ggcgaaccag gctgcggtcc tgctccttct gccgctggtc
     3301 atcgggcaga aaattgacat tgagtgtgcc ggagtcgatt tcggtgaggt gccggtactg
     3361 cacgcccaga tcgacgccac gcagtgcata gatcgtcggc gtgatcgtgg cgtcctggtt
     3421 gggcgcgata ttccaatacc agggtgtcgc gaccgtgatg ccgttggggc tggaggtact
     3481 gaactccgga aacaggaagc cgctcttgcg cgcatcgctc agtgggaagg aaatccacgg
     3541 tgtatagagc accggtatgc cttcgatctc gagccgggcg tcgcggccca caccttcgcc
     3601 ccgagcggtg tcgagcttca gctgacgggc acggatacgc cacactgctt tcggcggcgg
     3661 acagctcgtg tagaccacgt tctgcaccgc gatcacgttg tccggtgtcc agacgaatct
     3721 gtcagcgctg ccgtaaccgg gctgcttctg aaactggaac tgagcatggg tgaacatcgc
     3781 accatcgtcg gtgtagcgtc cggcatctcc cagcaccatc acgatcggat ttcggaagcg
     3841 cacctggccg accgcgttca tcgtgttgtt ggtgttgtca cgggtgatct gatcggcctg
     3901 aagctcgcga tcaccaatgt gaacatcgac atcgccagtc aggaccgaat agccgttcag
     3961 cagatcgccg gtcgcaccct gggcggtgaa gctgatgtca tcgccgatct gaaatgttct
     4021 cttcagcccg ggcggcgggg ccgcgtcggc ggctgcgagc aacggcatca gtgcagtcag
     4081 atagccgtag ccacccgaac agctttcagt gccagcctcg ctgcttgcat cggccgccac
     4141 ggccctcatc catggagcac agcagagcac aacgagcggc accaagcgca gggaagcaat
     4201 catgtgggcc gtcattataa tgatccgtca caggactgcg gtccggatag agaaagcacg
     4261 ttgcaatgac aaacagcgat gagcgcgtcg cgctgatccg agcctggctg acttcagagc
     4321 tgcagctgcg cgtcagccgg ttggagccgg cgtcggccga tgccagtttt cgccgctatt
     4381 tccgtatctg ggaagacaac ggtgcgacgc ggatcgtgat ggatgcgccg cccgacaagg
     4441 aagacatcgg cccgtttctg caggt
//
LOCUS       DATMRM010000024         7191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_940441, whole genome shotgun sequence.
ACCESSION   DATMRM010000024 DATMRM010000000
VERSION     DATMRM010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940441"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..629
                     /locus_tag="VNZ06_00660"
     CDS             270..629
                     /locus_tag="VNZ06_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWW19288.1"
                     /translation="MSIIGIDHVQLAIPEGGEDRARSFYGYVLGLPETPKPAELAKRG
                     GCWFESAHVKIHLGVDPGFRPGSKAHPALLVTDIQRIVERCLELSVQVVDEPMDGYRR
                     VYIHDPFGNRLELMEKK"
     gene            701..1147
                     /locus_tag="VNZ06_00665"
     CDS             701..1147
                     /locus_tag="VNZ06_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19289.1"
                     /translation="MTSASKHTVTGGCHCHNVAVTMQLSRSPEFYHPRACDCDFCTRH
                     GAAYISDAEGVLAIRIHDKRAVGKYLQGDQLAEFVLCRTCGVLIGVVYQSGERLYAAV
                     NANIVGTGSGFGERQASSPKQLSASEKTKRWKSVWFAEVAVSGFGA"
     gene            1152..1763
                     /locus_tag="VNZ06_00670"
     CDS             1152..1763
                     /locus_tag="VNZ06_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWW19290.1"
                     /translation="MKLYNEENPAPNPRRVRIFMAEKAITIPLVHVAMRERAHKSPEF
                     VAKNSLGQVPLLELDDGRLLSESVAICRYLEELHPAPALFGRDAWERARVEMWTRRIE
                     FALMNPISAVWVHTHRYTARLGTQYKDYGEAMRQRTMQRLGWLNDELEGQEFVAIDSY
                     SIADIVALTTIDFGKWIGIDIPVELTHLQAWHARVCARPSAIA"
     gene            1930..3198
                     /locus_tag="VNZ06_00675"
     CDS             1930..3198
                     /locus_tag="VNZ06_00675"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWW19291.1"
                     /translation="MPRLEFDGKQYPDGASSDPVALGWMLGTPPLAGKRILFETDRFF
                     DFPQIRWTLSHMRELVPTTSVWRGTTSARSLSSPEPARMSSIDALEFEDLNGRRLKWA
                     DSLSSTYTDGIIVLHRGVNVYERYFGALVPHRQHACFSIAKSYAATLAATLVYERALD
                     EKRTVVHYLPEMAHTAYADATLRDVLDMQVAVDFSEVYADPSADIWQYGRAGGMRARS
                     RDYAGAGNFYDYLVTLRKQGEHGAAFAYKTTNTEVLCWIMRRATGLALADMLSDRLWS
                     RLGCEEDGYITVDSIGVASGGAGLYGCLRDLARFGEMMRCEGSCRGQQLIPAAVIDDI
                     ARGSDRAKFEKAGYTLLQGYSYRNMWWISHDALGVYEGRGIHGQRLYIAPRAEVVIAR
                     FASHPVAASAANDPITLPSFRMLARHLMGA"
     gene            complement(3218..3544)
                     /locus_tag="VNZ06_00680"
     CDS             complement(3218..3544)
                     /locus_tag="VNZ06_00680"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWW19292.1"
                     /translation="MSTAFKASRDVIIRTHDLEKATRFYADVMHLPVIDRSASMNCVD
                     AGAFRLYIEQGPKHGPVFDFLVPNFNDAKRLLVEAGCVVVEDNPSVPRCYLADPHGLI
                     FNIAQA"
     gene            complement(3563..4972)
                     /locus_tag="VNZ06_00685"
     CDS             complement(3563..4972)
                     /locus_tag="VNZ06_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWW19293.1"
                     /translation="MKDDNSTQVVTQLLRRLATEGTPGARLPSVRELQQQHRVSPLTV
                     KHAMTPLIAEGLIEALPGHGTFVRARPGFRTGGEDFAWQSVILGVGRPSGETLGSLIA
                     LPTPGAITLTLGYLPADLQACAQLTASLTRAARRPAVWDRLPIEGLESLRGWFAAQLQ
                     PGLSPGDVQICPGSQAAIATTFRALTSPGDAVLLESPTYVGALAAAQAARLRVIPVPT
                     DAQGVRPDLLREALHTTGAKVFYCQPSHSNPSGSILSAPRRTEVLEAINAHNAFLIED
                     DWARDLGFEGTTPLPLATQDPGGHIIYIRSLTKASAPGLRIGALIARGPARARLKAAR
                     TIDDFYVAGPLQEAALQLLTSPVWARHLRAMRAALLQRRDALLAALRTHLKASSIDHV
                     PRGGFHLWLRLPPDVADLDVTHAAARAGVIISPGRYWFPAESTSSFVRLTFAAAPAEQ
                     LRRAAKLLATVLATGEVSA"
     gene            5144..5308
                     /locus_tag="VNZ06_00690"
     CDS             5144..5308
                     /locus_tag="VNZ06_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19294.1"
                     /translation="MAWGGRAAALGGGRDFEHALLASQNHIVLNPDALAKCRERHEKE
                     ISSSCRMFGS"
     gene            complement(5427..6674)
                     /locus_tag="VNZ06_00695"
     CDS             complement(5427..6674)
                     /locus_tag="VNZ06_00695"
                     /inference="COORDINATES: protein motif:HMM:NF004834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWW19295.1"
                     /translation="MGPTHEVQCCIVGGGPAGMLLGYLLGRAGVKTLLLEKHADFLRD
                     FRGDTVHPSTLRIMEELGLLDDFLSLPHSKIRSLSAEVDGHVLHLADFGKIAGRCNFV
                     ALMPQWDFLNFLASKAQRYPTLHVAMSCQATSLIEDPATGDVIGVNATGSAGPLQIRA
                     DLVVGCDGRSSTVRACAGLPVEILGSPIDVLWFRLSKKQTDRVDILGHLGHSRMLVTI
                     DRGDYWQCATVIAKGGIDRVRAAGIEAFRGSIAQSAHFLSDRVHELKSFDDVKLLSVM
                     VDRLTDWSQPGVLCIGDAAHAMSPVGGVGINLAIQDAVATANLLAAKLRAGTVRSVDL
                     ERVRRRRLWPVRVIQRLQVTIHNQVLNPVISSAPRVLSVPLPLRLVDRFPGLRGWPAQ
                     LLGVGVRPEHVHSPRERAGATHG"
     gene            6807..>7191
                     /locus_tag="VNZ06_00700"
     CDS             6807..>7191
                     /locus_tag="VNZ06_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19296.1"
                     /translation="MNVKRSLSLFGVGVVAVTASLSAPTQSVNDASPGIPHVRVFGGR
                     NAASPAYASSAKFDATLADLSAHLTQVRSGHEIADLHAMNPAIRVRQDPGSSQPLVLI
                     DAVTRGDPQGLRASLENLGLRDPSVY"
BASE COUNT         1452 a         2070 c         2279 g         1390 t
ORIGIN      
        1 gtgcttcatt gcgggaagca gtgattgggg tacctatcag gtacccggtg cgcttgagtc
       61 gatgacgtcg accagttgtg ccaggtttca gggcgcacac ttggtcgacg gtgcggggca
      121 ctgggtccaa caggaacagt cagagcgagt aaacgcgcta ctgttgcgct tcatggctaa
      181 ttatgcatca cttgcataaa gggttcgttt cggtcgatat ttcctatcat gtgaggcgat
      241 tcgatacact catctcaagg agatgagtca tgtcgatcat cggcatcgat cacgttcagc
      301 tggcgatacc cgagggcggg gaagatcggg cgcgttcgtt ctacgggtat gtgctaggac
      361 tgccggagac tccgaagccg gctgagttag ccaagcgggg cggctgctgg ttcgagagtg
      421 ctcacgtcaa gatccactta ggggtcgatc cggggtttag gcccggaagc aaggcgcatc
      481 cggccctgtt ggtgacggat atccaaagaa tagtggagcg atgtctggaa ctgagcgttc
      541 aagtggtcga tgagccgatg gatggttatc gccgggttta tatccacgat ccgttcggca
      601 accgactcga gctgatggaa aaaaagtagt ccacccgtaa tcggcacagc ttgcaacccc
      661 ggcatcggcc agcagtgttc ggacaatgga ggattcgcca ttgaccagtg ccagcaaaca
      721 taccgtgact ggcggatgcc actgtcacaa tgtcgcggtg acgatgcagc tctctcgttc
      781 gcctgagttc tatcatccgc gagcctgcga ctgcgacttc tgcacgcggc acggcgccgc
      841 atacatatcc gatgccgaag gggtgctggc gattcgcatt catgacaagc gcgccgttgg
      901 caaataccta cagggtgatc aactggctga gttcgtgctt tgtcgcacct gcggcgtgct
      961 tatcggggtg gtgtaccaat ccggggagcg gttgtatgcg gcggtcaacg ccaatatcgt
     1021 cggcaccgga tcgggatttg gagagcgaca ggcttcttcg ccgaagcagc tatcagcctc
     1081 ggaaaagacg aaacgctgga agagcgtgtg gtttgcagaa gtggctgtca gcggtttcgg
     1141 agcgtagttg agtgaagtta tacaacgaag aaaatcccgc tccgaatccg cgccgcgtcc
     1201 gtatattcat ggccgaaaag gccataacga ttccgctggt gcatgtagcg atgcgcgaac
     1261 gcgctcataa gagtcctgag ttcgttgcca agaattcgct cggacaggtg ccgctactgg
     1321 agctggatga tggcaggctg ctgtctgaga gtgtggcgat ttgccgctat ctggaggagt
     1381 tgcatccggc gcctgctctg ttcggtcgcg atgcatggga gcgcgcgcgc gtggagatgt
     1441 ggactcgccg aatcgagttc gcactgatga atccaatctc ggcggtttgg gtccatactc
     1501 accggtacac tgctcgcctg gggacccaat ataaagacta tggtgaggcg atgcgccagc
     1561 gcaccatgca gcggctgggc tggttaaacg acgagctgga gggccaagaa ttcgtggcca
     1621 ttgacagcta ttcgatcgcc gacatcgtcg ctttgacgac gattgacttc gggaagtgga
     1681 tcggcatcga cataccggtg gaactgacgc atctgcaagc atggcacgcg cgagtctgcg
     1741 cgcggccgag cgccatcgca tagcttgcag cgagtcgtgc accgccgtgt gtgcgcagtg
     1801 acttgatctc gaagttgcgg aacagcatcg acgtcaccag gctaatctca agcaacgcca
     1861 gatttgccaa aatacgttcc gggggctaat agatccagtg ccttcagtga accctggagt
     1921 aagtgccaga tgcctcgact cgaatttgac ggtaagcaat atccggacgg cgcgtcgtcg
     1981 gatccggtag ccctgggttg gatgctgggg actccgccgc tggcgggtaa gagaatactg
     2041 tttgagaccg accgcttttt cgattttccg cagatccgat ggacgctgtc gcacatgcgc
     2101 gaactggtgc cgacgacgag tgtatggcgc ggaaccacgt ccgcacgctc actgagttcg
     2161 cccgaacccg cgaggatgtc gtcgatcgac gcgctggagt tcgaagatct gaacggtcgc
     2221 cggctgaagt gggcagactc gctctcgtcc acctataccg acgggatcat tgttctgcac
     2281 cgcggcgtca atgtgtatga acgctatttc ggtgcgctcg tgccgcatcg acaacacgcc
     2341 tgtttctcca ttgccaagtc ttacgcagcg acgctggcgg ctacgctcgt ctacgagcgc
     2401 gcgctggacg agaagaggac tgtggtccat tacttgccgg agatggctca taccgcttac
     2461 gcagacgcga ctctgcgcga tgtgctcgat atgcaggtag ccgtggattt cagcgaggtc
     2521 tacgccgatc cgtcagccga catctggcag tacggtcggg cagggggaat gcgagcgcgt
     2581 tctcgcgact acgctggagc gggaaacttc tacgactacc tggtgacctt gcgaaaacaa
     2641 ggggaacatg gcgcagcgtt tgcttacaag acgaccaata ccgaggtgct gtgttggatc
     2701 atgcgacgcg ccaccgggct ggcgctggcg gacatgctga gcgaccgact ctggtcccgt
     2761 ctgggatgcg aggaagatgg ctatatcacc gtcgattcga tcggcgtggc ctcaggcggc
     2821 gccggactgt acggttgctt gcgggatctc gctcgattcg gagagatgat gcgctgtgag
     2881 ggcagctgtc gtggacagca gctcataccc gccgcggtga tcgacgacat cgcacgagga
     2941 tctgatcgcg cgaagttcga gaaggctggc tacacgctgc tgcaggggta ttcctatcgc
     3001 aacatgtggt ggatctcgca cgacgcattg ggcgtgtacg agggccgtgg cattcacgga
     3061 caacgcctgt acatcgcacc ccgagccgaa gtagtgatcg cacgcttcgc gtcacatccc
     3121 gtggcggcga gtgctgccaa cgacccgatc accctgccga gcttcagaat gctcgccagg
     3181 catctgatgg gcgcctgaac cgggctgtcg gctctgatca ggcctgggcg atgttgaaga
     3241 tcaggccgtg cggatccgcc agataacagc gcggtaccga cgggttgtct tccaccacga
     3301 cgcaacccgc ttccacgagc aagcgttttg cgtcgttgaa gttcggcacc agaaaatcga
     3361 ataccggacc gtgcttcggg ccctgttcga tatacaagcg aaacgcaccc gcgtcgacac
     3421 agttcatcga cgcgctgcga tcaatgaccg gtaagtgcat cacgtcggca tagaagcgag
     3481 tcgccttttc cagatcatgg gtacgaatga tgacgtcacg agaagcttta aaggctgtgg
     3541 acacgtcgaa atcctcctcg ggtcaagcag agacttcccc ggtcgcgagc accgtggcca
     3601 gtagtttagc cgcgcgacgt aactgttcag caggcgcggc tgcgaatgtg aggcggacga
     3661 aggaagaagt ggactccgcc gggaaccagt atctgccggg gctgatgatg acgccggcac
     3721 gtgcggcggc gtgagtgaca tccagatcgg cgacgtcggg cggaagtctg agccatagat
     3781 gaaagccgcc gcgcggcacg tggtcgattg acgaggcttt gagatgagtc cgtagcgccg
     3841 ccagcagcgc gtcgcgtcgt tgcaggagcg ctgcgcgcat ggcacgcaga tgtcgggccc
     3901 agacgggtga agtcaatagc tgcagtgcgg cctcctgcaa cgggcctgca acgtagaagt
     3961 catcgatcgt gcgagcggcc ttcaggcgag cccgcgcagg tccgcgcgcg atgagtgcgc
     4021 cgatgcgcag ccccggggca gaggccttcg tgagcgatcg gatatagatg atgtggccac
     4081 ccggatcctg ggtggccagt ggtagggggg tggtgccctc gaatcccagg tcgcgcgccc
     4141 agtcgtcctc aatcaggaac gcattgtgag cgttgatcgc ctccaatacc tccgttcgcc
     4201 gcggcgcact gagaatcgaa cccgatggat tggaatgcga cggctggcaa tagaatacct
     4261 tggcaccggt agtgtgcagc gcctctcgca acaggtcggg acgcacccct tgcgcatcgg
     4321 tcggcaccgg gatcacgcgc agccgcgcag cttgcgctgc agcgagtgcg ccgacatagg
     4381 taggactttc cagcagcacc gcatctccgg gtgaggtcaa cgcccggaaa gtcgtcgcaa
     4441 tggctgcctg gctccctggg cagatctgca cgtcgccagg gctaagtccc ggctgcaact
     4501 gcgcggcgaa ccatccgcgc aatgactcca gaccttcgat cgggagtcga tcccataccg
     4561 cgggccgtcg cgccgcacgt gtcagcgatg cggtgagctg tgcacaggcc tgaagatcgg
     4621 ccggcagata tcccaaggtg agtgtgatgg ctccgggtgt tggtagcgca atgagcgaac
     4681 ccagcgtctc gccgctgggt cgtccgacac ccaggatcac actctgccac gcgaagtcct
     4741 cgccgccggt gcgaaatccg gggcgagcgc gcacgaacgt gccgtgtccg ggcaacgctt
     4801 cgatcaagcc ttcggcaatc aacggcgtca tcgcgtgttt gacggtcaat gggctgacgc
     4861 ggtgttgctg ctgcagctca cgcaccgatg gcagccgcgc cccgggtgta ccctccgttg
     4921 ccaatcgccg cagaagctga gtaacgactt gcgtactgtt atcgtctttc atgccactcg
     4981 atgataacga tatcgcagca cgggtagaag cgttaccgct ccggccacgc gcaaattcgg
     5041 gtggtttact gtgggtgccg tcgggttcgt cgcgttcgtc gcgttttctc tgtcacactg
     5101 cttgccacca agatcgcggt tcagacgctc gacggcagag tcgttggcct ggggcgggcg
     5161 agcggccgcg ctgggaggcg gccgcgactt cgagcatgca ctgctggcgt cgcagaatca
     5221 catcgtgctc aatcccgatg cgctggcgaa atgccgcgag cgccatgaga aggaaatctc
     5281 gtcaagctgc aggatgttcg gaagctgatg gcgaaaggac atatccgcga agccgcatcg
     5341 ttgctgaaaa agatcgacgc tcactacgcg ggacttgcgg cccctgagtc tgtcgagctg
     5401 gccagacaga tcgcagcgtc cctgcactag ccgtgtgtcg caccggcgcg ctcgcgcggg
     5461 gaatgcacat gttccggacg cacacccact cccagcagct gggccggcca gccccgcagc
     5521 cccgggaatc gatcgacgag cctcaatgga agggggacgc tcagcacgcg cggtgcggaa
     5581 gaaatcacgg gattgagtac ttgattgtga atcgtgacct ggaggcgctg aatgactcgc
     5641 actggccaca agcggcgtct tcgcacccgc tcgagatcaa cgctgcgaac ggttccggcc
     5701 ctgagtttgg ctgccagcaa attggcagtc gcgacagcat cctggatggc cagattgatc
     5761 ccgacaccgc cgaccggaga catcgcatga gccgcatcac cgatgcacag cacgcccggc
     5821 tgggaccagt ccgtcagccg atcgaccatg acactgagca atttgacgtc atcgaagctt
     5881 tttaattcgt gcacacggtc ggacagaaag tgagcgctct gcgcaatcga gcctcgaaac
     5941 gcctcgatgc ccgctgctcg cacgcgatcg atgccgcctt tggcgatcac ggtcgcgcat
     6001 tgccagtagt cgccgcgatc gatcgtcacg agcatccggc tgtgacccag gtgtcccaga
     6061 atatccaccc gatccgtctg tttcttggac aagcgaaacc acaacacatc gatcggagaa
     6121 ccgaggattt ccaccggcag ccccgcacaa gcccgcacgg tcgaactgcg gccatcgcag
     6181 ccgaccacca gatcggcgcg aatctgcaac gggcctgcgg atccagtcgc gttgacgccg
     6241 atgacgtctc cggtggcggg gtcttcaatc aggctggttg cctgacaaga catagcgaca
     6301 tgcagggtcg gatagcgctg tgctttcgag gcaaggaaat tcagaaagtc ccattgaggc
     6361 atgagggcga cgaagttgca gcgtcctgca attttcccga agtcagccag gtgtaggacg
     6421 tggccatcca cttccgccga tagtgatcgg atctttgagt gaggaagcga caagaagtca
     6481 tcgagcagcc ccagttcctc catgatccgc agcgtcgacg gatgtacggt gtccccgcgg
     6541 aagtcgcgca ggaagtccgc gtgcttttcg agcaggagag tcttgacgcc tgcgcgtccg
     6601 agcaaatagc ccagcagcat gccggcgggg ccacccccga cgatgcagca ctgaacctca
     6661 tgcgtaggcc ccatctgtat gcctcgccta tgtccttgag cttcagagta tgaaaggaat
     6721 gagacaggta gggtagggcg ggccgctggt acactttgtc cgacgattca accactaaaa
     6781 cgggacaggg cacctcaaga gtaaccatga atgtaaaacg gtctctgagt ctcttcgggg
     6841 tcggggtggt ggcggtcact gccagcctgt cagcgccgac acagtcggtc aacgatgcgt
     6901 cgccgggcat cccgcatgtg cgcgtcttcg gcggtcgcaa cgcggcatcg cctgcgtacg
     6961 cgagcagtgc caaattcgac gcgacgctgg cggatctgtc ggcgcatctg acgcaagtgc
     7021 gatccgggca cgaaattgca gacctccatg ccatgaatcc ggcgattcgg gtgcggcaag
     7081 atcctggcag ttcacagccc ttggtgctga tcgacgcggt gacccgtggc gatccgcagg
     7141 gacttagggc ctcgttggag aatttgggtc tgcgtgaccc ttccgtgtac g
//
LOCUS       DATMRM010000025         4010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_322705, whole genome shotgun sequence.
ACCESSION   DATMRM010000025 DATMRM010000000
VERSION     DATMRM010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322705"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..462
                     /locus_tag="VNZ06_00705"
     CDS             52..462
                     /locus_tag="VNZ06_00705"
                     /inference="COORDINATES: protein motif:HMM:NF025705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HWW19297.1"
                     /translation="MRVLGLLLALGCTAALADQLPSAPGTEVYIVSPHAGAKVKSPVT
                     VVFGLKGMGVAPAGVKFDNTGHHHLLLDCAVPTDLTAPLPATDAIRHFGKGQTETSIE
                     LKPGKHTLQLLFADYTHTPFMPNVVSKPITITVE"
     gene            495..2216
                     /locus_tag="VNZ06_00710"
     CDS             495..2216
                     /locus_tag="VNZ06_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWW19298.1"
                     /translation="MYLAFFGLAEKPFSITPDPRYLYLSARHADALAHLVYGINEAGG
                     FVQLTGEVGTGKTTIIRSLLSRTPQNAEIALILNPRLSPTEFLLSLCEELGIGAADSP
                     DGTAKDIVDLLNRYLLRAHAQGRRVVLIVDEAQNLAPEVLEQIRLLTNLETETQKLLQ
                     IILIGQPELRRLLAREDLRQIAQRVTGRYHLDPLTRTEAAAYVRHRLRVAGATADIFS
                     SGALREIYHISHGIPRVINIICDRALLGAYTQDLHQVPASLVRRASAEVFDHDIAPSW
                     MPVLVASMAILIIGGSGALLWRYVPSWHRSASANAAPVASSVPSATLPAAPAANLPAL
                     LSTTPSVADHPPPPVSLPQSAPMPELPHVLADNRTSTDADTAFHQLLQLWKANYTPGK
                     LDGCSQAIAQGLRCLNQRGSLAQLRQLNRPAILLLTDDSGSAYQVVLRQMGDSTAQLQ
                     LGDQTVTINLSELTRYWYGDFVLLWHPVSRDVRDLSAGMRGSQVRVLRSLLNDWSGAN
                     TAGASSDEYDESLVKMVEQFQRANRLQVDGIAGIETQIALDSALATPDSPLLQSRPMQ
                     QASTRGT"
     gene            2216..2899
                     /locus_tag="VNZ06_00715"
     CDS             2216..2899
                     /locus_tag="VNZ06_00715"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HWW19299.1"
                     /translation="MSLILDALKKSEAERQRQSGPTLLEVRVARPQRRYPLWALAVAI
                     LLAVNMVLLLVFVLRPSDSGRASSLTASTTTLQPPPAVTAPASPASALSAPAATMPAQ
                     PAAAQPGTSSSASPTLADNSTDPGANPADNEPAISPIAAGLRAQRDMTRADYSTLPGI
                     NEMAANVPDLRLDLHVYAERPRDRYALINMQTVHEGDTLSEGPRVLAITRDGVALEWH
                     SQQFMLRPQ"
     gene            complement(2880..>4010)
                     /locus_tag="VNZ06_00720"
     CDS             complement(2880..>4010)
                     /locus_tag="VNZ06_00720"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWW19300.1"
                     /translation="PLSKKWLIEPGNRSSLALRGAEALARRRITLRLKATVRALDRAG
                     SQLEFADGERVRYSKLALATGSIPRALPLDGASLEHVLSLRSIEDCVAISEALRRCAS
                     EASQVVVIGGGFIGLEVAAGARKLGAQVTVLEGLPRLMSRVTAPVISQAFERLHRAHD
                     VNLVFDARVSELVGHEGRVCAVRTADGREYPAGCVVVGIGVLPDDRLAKTAGLDCDHG
                     IIVDDCARTSDPAIAAAGDCTARRLADGRLLRLESVQNAVEQGKSAAAALLGKERPFV
                     AAPWFWSDQFEVKLQMVGLSTGYDQVVTRGDLQKLAFSAFYFRDGRLIAVDSLSRVQD
                     HMQAKRLLDAGLSCTPEQAADPQFELQSLVNKAKQAATAGGA"
BASE COUNT          723 a         1333 c         1245 g          709 t
ORIGIN      
        1 catacttggc gagcgaccga ctctttccca aacaccccaa ggagaagacg aatgcgagtt
       61 cttggactac tgctggcgct aggttgcacc gctgcgctgg ccgatcagct gccgagcgcg
      121 cccggcaccg aggtctacat cgtatcgccc catgccgggg cgaaagtgaa gagtccggtc
      181 acggtggtgt tcggtctgaa gggcatgggc gttgcgcccg ccggagtgaa gttcgacaac
      241 accggtcacc atcatctgct gctggactgc gccgtgccga cggatctgac cgcgccgctg
      301 cccgccaccg atgcgatccg gcacttcggc aagggtcaga ccgagacctc gatcgaactc
      361 aagcccggca agcacacact gcagttgctg tttgcggact acacgcacac gcccttcatg
      421 cccaacgtcg tgtcgaagcc gatcacgatc actgtcgaat gacgaatgca gcgcgtacca
      481 ggtgatgctc gatcatgtat ctggcatttt tcggactagc agaaaagcct ttctcgatca
      541 cgcctgatcc gcgctatctc tacctgagcg cgcgccacgc ggatgcgctg gctcacctgg
      601 tgtatggcat caacgaggcc ggcggcttcg tacagctcac gggagaagtc ggcactggca
      661 agacgacgat catccgctca ctgctgtcgc gcaccccgca gaacgccgag atcgcgctga
      721 ttctcaatcc ccggctgtcg ccgacggaat ttctgctgtc gctctgcgaa gaactgggca
      781 tcggcgccgc agacagtccg gatggcaccg ccaaggacat tgtggacctg ctcaatcgct
      841 atctgttgcg tgcgcacgcg caaggacggc gtgtggtgct gatcgttgac gaggcacaga
      901 acctcgcgcc cgaggtactg gagcagattc ggctgctgac caatcttgaa accgaaaccc
      961 agaaactgct gcagatcatt ctgatcgggc agcccgaact tcgccgtttg ctggcgcgcg
     1021 aagacttgcg tcagatcgct cagcgcgtca ccgggcgcta ccatctcgat ccgctgacac
     1081 gcaccgaagc ggccgcgtat gtccgacacc ggctgcgggt tgcgggagcc acggcggata
     1141 ttttcagcag cggcgcgctg cgggagatct atcacatcag ccacggcatt ccgcgcgtca
     1201 tcaacatcat ctgcgatcgc gcgttgctgg gcgcgtacac gcaagatctg caccaggtgc
     1261 cggcttcgct cgtcagacgc gccagtgcag aagtgttcga ccatgacatt gcgccaagct
     1321 ggatgccagt gctggtggcg agcatggcga tattgatcat cggcggcagc ggtgcactgc
     1381 tgtggcgcta tgtgccgagc tggcatcgca gcgcgagcgc gaatgcagcg cctgtggctt
     1441 cgagcgtacc ctccgctacg ttgccggccg ccccggccgc gaacctgcca gccctgctgt
     1501 ctaccacgcc gtccgtggcg gaccatccac cgcccccggt gtcattgccc cagtccgcgc
     1561 caatgccgga gttgccgcat gtactggccg acaaccgcac gagcaccgac gcggataccg
     1621 cattccacca gctgctgcag ctgtggaagg ccaactacac gccgggcaag ctcgacggct
     1681 gcagtcaggc gatcgctcag ggactgcgtt gcctgaatca gcgcggatcg ctggcgcagt
     1741 tgcggcaact gaatcgaccc gccatcctgc tgctgaccga cgattccggc agcgcgtatc
     1801 aggttgtgct gcgtcagatg ggtgattcga ccgcgcagct gcagctgggt gatcagaccg
     1861 tgacgatcaa cctttcggaa ctgacgcgct attggtacgg tgattttgtt ctgttatggc
     1921 acccggtttc gcgcgatgtg cgcgacctga gtgctggcat gcgcggctcg caggtgcggg
     1981 tgctgcgctc attgctcaat gactggagcg gggccaacac cgcgggcgcg agcagcgatg
     2041 aatacgatga aagcctggtg aagatggtcg agcagtttca gcgagccaat cggctgcagg
     2101 tggacggcat cgccggcatc gagacgcaga ttgcgctcga ctccgcgctg gccactccgg
     2161 attcgccgct gctgcagagc cgaccgatgc agcaggcatc cacccgaggc acctgatgtc
     2221 gctgatcctc gatgcgctca agaagtccga agccgagcgg caacgccaga gcgggccgac
     2281 cctgttggag gtgcgggtcg ccaggcctca gcggcgctac ccgctgtggg cacttgcggt
     2341 ggcgattctg ctggccgtca acatggtcct gttgctggtg ttcgtgctgc gaccgtctga
     2401 tagtggacgc gcctcgagcc tcacggcgtc gaccacgacg ctgcagccac cgcccgcagt
     2461 gactgctccg gcctcgccgg ccagcgcatt gtccgcccca gcggcgacga tgcccgcgca
     2521 accggccgcg gcccagcccg ggacgtcgtc ttccgcgtcg ccaacgctcg cggacaactc
     2581 gaccgatccg ggcgccaatc cggccgacaa tgagcccgct atctccccga tcgccgccgg
     2641 cttgcgggct cagcgcgata tgacgcgcgc ggactattca acgcttccgg gcattaacga
     2701 gatggcagcc aatgttccgg atctgcggct cgacctgcac gtctacgcgg aacgcccgcg
     2761 cgatcggtac gcgctcatca atatgcaaac cgttcacgaa ggggacaccc tgtcggaggg
     2821 accgcgggta ctggccatca cgcgcgatgg tgtcgcgctc gagtggcaca gtcagcaatt
     2881 catgctccgc ccgcagtagc ggcctgtttc gctttgttga ccaggctctg cagttcgaac
     2941 tgcgggtcgg ccgcctgttc aggcgtgcag gataggccgg cgtccagcag ccgcttggct
     3001 tgcatgtgat cctgtacgcg gctgagcgaa tcgaccgcaa tcaaacgccc gtcgcggaaa
     3061 taaaacgccg agaacgcgag tttctgcaga tcaccacggg tgaccacctg atcgtaaccg
     3121 gtactcaatc caaccatctg cagcttgacc tcgaactgat cggaccagaa ccagggagca
     3181 gcgacgaacg gtcgctcctt tcccaacagt gcagccgccg cagattttcc ctgttccacc
     3241 gcgttctgaa cggactcgag acgcagcagt cgtccgtccg ccaggcgccg ggcggtgcag
     3301 tcgccggccg cggcgatcgc cggatcgctg gtgcgggcac agtcatcgac gatgatgccg
     3361 tgatcgcagt cgaggcctgc cgttttggcc agccgatcgt ccggcagcac gccgattccg
     3421 accacaacac agccggcggg gtattcgcgg ccgtcggcag tacgcaccgc gcacacgcgc
     3481 ccttcgtggc cgaccagctc gctgacccgc gcgtcgaata cgaggttcac atcgtgtgcg
     3541 cgatgcaggc gttcgaatgc ctgcgagatg accggagcgg tcacgcgcga catcagtcgc
     3601 ggcaggcctt cgagcaccgt gacctgcgca ccgagtttgc gcgcgccggc cgccacttcc
     3661 aatccaatga agccgccacc gatcacgacg acctgcgatg cttcgctggc gcagcggcgc
     3721 agcgcctcgc tgatggcaac gcagtcttcg atcgagcgca gactcaggac atgctcgagt
     3781 gaggccccgt cgagtggcaa cgcacgtggt atggaccccg tggccaatgc cagcttgctg
     3841 tagcgcacgc gctctccatc tgcgaactcc agctgtgagc cggcgcgatc gagcgcgcgc
     3901 accgtagcct tgagcctgag ggtgatcctt cgccttgcca gcgcttccgc gccgcgcagg
     3961 gccagcgagc tgcggttacc cggctcgatc agccatttct tcgacagcgg
//
LOCUS       DATMRM010000026         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4041328, whole genome shotgun sequence.
ACCESSION   DATMRM010000026 DATMRM010000000
VERSION     DATMRM010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4041328"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /gene="hisB"
                     /locus_tag="VNZ06_00725"
     CDS             <1..986
                     /gene="hisB"
                     /locus_tag="VNZ06_00725"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HWW19301.1"
                     /translation="LRKVRLLPDVIAPLLELKAAGYRFVMVTNQDGLGGERYPQQRFD
                     QVQQFVLQLFGSQGIEFERIFICPHSEHEGCACRKPLTGLVTEYLQSIQLDRARSAMV
                     GDRDTDLQFARNLGIRGFRVHPPQESWPSIAHALLTRRASVHRKTRETDIAAEIELDS
                     TAGCEIHTGIGFFDHMLEQLAKHGGFGLKLRCSGDLHIDEHHTVEDCALTLGEALRQA
                     LGDKRGIERYGFLLAMDESSAQVAVDLSGRPYFVFEGAFARDQVGALPTELVPHFFRS
                     LAQSLGAALQITVRGENTHHMIEACFKGVGRALRPALRREGRDLPSTKGAL"
     gene            983..1576
                     /gene="hisH"
                     /locus_tag="VNZ06_00730"
     CDS             983..1576
                     /gene="hisH"
                     /locus_tag="VNZ06_00730"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HWW19302.1"
                     /translation="MSEVAIIDSGGANLASLQYALERLGAASIVSDDPERIASARRVL
                     LPGVGAAGDAMQRLRARGIDEVIPCLKVPLLGICLGMQLLFEHSSEDDTPCLGVLPGH
                     IERLQPSVGRPVPHMGWNTLESIGEDVLLRDIGRDDHVYFVHSYAAPVSHTTLAAARY
                     GEAFAAVVRRDNFRGVQFHPERSGRVGARLLQNFLTL"
     gene            1586..2338
                     /locus_tag="VNZ06_00735"
     CDS             1586..2338
                     /locus_tag="VNZ06_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HWW19303.1"
                     /translation="MLLIPSIDLRGGKCVRLLKGDFTAETCYEFGALELMWRYRQLGA
                     AWLHLVDLDAARDGTLTRDNPNRAMIQQLAAPRALALQVGGGLRSRAALEALFAIGVA
                     RAVIGSAAVEQRAEVAQWLSAYGPERICLAFDVRMDAQQIPRLRIRGWQQGTDLSLWD
                     AVESFRDSGLKHVLCTDIECDGALAGPNLALYEEALRRFPDIRWQASGGVSTGADLIA
                     LAAHGVPAAISGRALLEARISSEELRPFLPNA"
BASE COUNT          382 a          777 c          777 g          415 t
ORIGIN      
        1 cactgcgcaa ggtgcgtctg ctgcccgatg tgatcgcgcc gctgctcgaa ctgaaagcgg
       61 ccggctatcg cttcgtcatg gtcaccaacc aggatgggct cggcggcgag cgctacccgc
      121 agcagcgttt cgatcaggta cagcagttcg tgctgcagct gttcggctcg caaggcatcg
      181 aattcgagcg catcttcatc tgtccgcact ccgagcatga gggctgcgcc tgtcgcaagc
      241 cgctcaccgg cctcgtgacc gaatacctgc agagtattca gctcgatcgc gcgcgcagcg
      301 cgatggtggg tgatcgcgac accgacctgc agttcgcgcg caatctgggc atccgtggat
      361 ttcgcgtgca cccgccacag gagagctggc cctcgatcgc acacgcgctg ctcacccggc
      421 gcgccagcgt gcaccgcaag acgcgtgaaa ccgatatcgc ggccgagatc gaactcgaca
      481 gtaccgcggg ctgtgagatt cacaccggca tcggcttttt cgatcacatg ctcgagcagc
      541 tggccaagca cggcggcttt gggctcaaac tgcgctgcag cggcgatctg cacatcgatg
      601 agcaccacac ggtagaggac tgcgcgctga cgctcggcga agccctgcga caggcactgg
      661 gcgacaagcg tggcatcgag cgctatgggt tcctgctggc gatggatgaa agcagcgccc
      721 aggttgccgt ggacctgtcc gggcgcccct acttcgtctt cgagggcgca ttcgcgcgcg
      781 atcaggtcgg cgcactcccc accgagctgg tgccgcactt ttttcgttcg ctggcccaat
      841 ccctgggggc agcgctgcag atcacggtgc gcggcgagaa cacccatcac atgatcgaag
      901 cctgcttcaa gggcgtcggc cgcgcgttgc gcccagcgct gcgacgcgaa ggtcgcgatc
      961 ttccgagcac caagggcgca ctgtgagcga agtcgcgatc atcgacagtg gcggtgccaa
     1021 tctggcatcg ctgcaatatg cgctcgaacg tctgggtgcc gcatcgatcg tgagcgacga
     1081 tcccgagcgc atcgcttcgg cccggcgcgt gctgctgccc ggcgtgggcg cggctggcga
     1141 cgccatgcag cgtttgcgcg cgcgcggcat cgatgaggtc attccgtgcc tgaaagtgcc
     1201 gttgctcggt atctgtctcg gcatgcaatt gttgttcgag cattccagcg aggacgacac
     1261 cccctgtctg ggcgtactgc ccgggcacat cgagcgcttg cagccaagcg tcggccgccc
     1321 ggtgccgcat atgggctgga acacgctcga gtccatcggc gaggacgtcc tgctgcgcga
     1381 catcggccgc gacgatcacg tgtacttcgt tcacagctac gcggcaccgg tgtcgcacac
     1441 caccctcgcg gcggctcgct acggcgaagc cttcgccgcg gtggtcaggc gcgataactt
     1501 tcgcggcgtg cagtttcatc cggaacgctc gggcagagtc ggcgcccggc tgctgcagaa
     1561 ctttctcacg ctctagccag cgcgcatgct gctcatacct tccatcgacc tgcgcggcgg
     1621 caaatgcgtg cgcctgctca agggcgattt caccgccgag acgtgctacg agttcggagc
     1681 gctggagctg atgtggcgtt atcggcagct cggcgcggcc tggttgcacc tggtggatct
     1741 cgatgccgcg cgcgacggca ccctcacgcg cgacaacccg aaccgagcca tgatccagca
     1801 gctggcggca ccgcgtgcgc tcgcgctgca ggtcggcggc ggcctgcgca gccgcgcggc
     1861 gctcgaggcg ctgttcgcga tcggtgtcgc gcgcgccgtg attggcagcg ccgccgtgga
     1921 gcagcgcgct gaggtcgctc aatggctgag cgcgtatggc ccggaacgca tctgtctggc
     1981 cttcgatgtc aggatggacg cccaacagat accgcgactg cgcatccgcg gctggcagca
     2041 ggggacggac ttgagcctgt gggatgccgt ggagtcgttc cgcgacagcg gactgaagca
     2101 cgtgctgtgc accgacatcg agtgcgatgg cgcgctggca ggaccgaatc ttgcgctgta
     2161 cgaggaggca ctgcgccgct ttccggacat ccgatggcag gcctcgggcg gcgtcagtac
     2221 cggcgcggat ctgatcgccc tcgccgccca cggcgtgcct gcggccatca gtggcagggc
     2281 gctgctggaa gcgcgcatct ccagtgagga actgcgccca ttcttgccaa acgcataatt
     2341 ccctgtctcg a
//
LOCUS       DATMRM010000027        12586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3423421, whole genome shotgun sequence.
ACCESSION   DATMRM010000027 DATMRM010000000
VERSION     DATMRM010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12586
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3423421"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VNZ06_00740"
     rRNA            complement(<1..867)
                     /locus_tag="VNZ06_00740"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1415..2704
                     /locus_tag="VNZ06_00745"
     CDS             1415..2704
                     /locus_tag="VNZ06_00745"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWW19304.1"
                     /translation="MSTGTDLPADTLGSPWLLRSLQLLACFAVISYTLQVARHTTPPT
                     LQLGTMLFGASDHAPGNPVDVNDISAVLAPGLDLAEVIVQRNDTLDHIFRRLQLSLSD
                     LADVRALSGVQTMLDRLSPGEHLKFLHRDAALIGLERHLSLTEKLEVRRTDAGFRATV
                     VPKAIQIDDSVAHGEINSSLFESANAAGLSDLSVLKLAKIFASDIDFVIGLREGDRFV
                     VSYERILQNDHYVKDGDILAARFINQGRVYEAVRYVGPDGAARYYSPDGHSTEKAFLR
                     APLEFKRISSGFSLARMHPILNLIRAHKGIDYAAPIGTPVYAAGSGHIKFRGDLGGYG
                     NVIQIEHGNGIVTVYGHMSRFAGARAGAHVERGETIGYVGMTGLATGPHLHFEFRVNG
                     QYVDPQKIKLPDAKPIDPDLYDDFKQKSAPLLAALNP"
     gene            2701..3810
                     /locus_tag="VNZ06_00750"
     CDS             2701..3810
                     /locus_tag="VNZ06_00750"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HWW19305.1"
                     /translation="MSLYVGLMSGTSMDGIDAVLLQIDEGGMRVKAAVARHWPADLQR
                     RLRQAAEDYLSLGLLEFGRLDTIVGREFASAAEELLNRSGTALSAVRAIGSHGQTVLH
                     QPGDAAPFTLQIGDPNIIAERLNIDVVADFRRRDLAAGGEGAPLMPAFHVAAFGTPGQ
                     YNAVANIGGIANVSRLQADGSVTGFDTGPGNCLLDAWSRQQRAKPFDEHGQWAASGVI
                     QPTLLNRMLQDPYFARRPPKSTGRETFSDGWLEQALAGYRASPADVQATLTELTAHTI
                     AMSLAGGGTQPVRVLVCGGGAQNTYLMSRLSLALPRSQVQTTGACGIDPQHVEAAGFA
                     WLAYRYVNALPGNLPSVTGAKYAVPLGALYRGSAK"
     gene            3918..5999
                     /gene="ppk1"
                     /locus_tag="VNZ06_00755"
     CDS             3918..5999
                     /gene="ppk1"
                     /locus_tag="VNZ06_00755"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HWW19306.1"
                     /translation="MDQPVFRAPQNFINRELSSLEFNQRVLAQSLDLRVPLLERLRFL
                     CISCANLDEFFEIRVAGLKQLLELGSAQLGPDGLGIAEQLSMIHERTTRLVHDQYACL
                     NDVLLPALSAAKIAVLDEADWDDTTRVWVEKHFLAEVLPVLSPLGLDPARPFPRIQNK
                     SLNFIVRLSGKDAYGRDSELAIVQAPRSLPRIVPLPDEGPTRRFVALSTIVEAFVAHL
                     FEGVQVLGCYQFRLTRNSDLFVDDEEVDDLRRALEGELAQRRYGAAVRLETAANCPAD
                     TVEFLLREFALSSRDCYQVPGPVNLNRFSAIYDLVQRTDLKYPTFTPGLPARLVGATD
                     LFAAIRQRDLLLHHPYRSFGPVMDLLRQAASDPAVLAIKQTLYRTGDHSPIVDALVAA
                     AMADKDVTVIIELRARFDEEANIELATRLQEAGAHVMYGVVGYKTHAKMTLIVRRETD
                     GMRRYCHLGTGNYHPKTARGYTDYGLFTCDEAIGSDVHEIFLQLTSLTRTPKLRRLLQ
                     SPFHLHEATLALIERESAHALAGKKARIIVKLNALVDPQTIETLYRASRSGVKIDLIV
                     RGVCAVRPGIPGVSENIRVISVVGRFLEHSRVYWLANDGSPELYLSSADWMERNFFRR
                     VEVAFPVLQPQHRERIVKDLETYLKDNTNAWELQADGTYARVNVSSAPTVDSQALLLE
                     RYAGTPAPPAP"
     gene            complement(5996..7522)
                     /gene="ppx"
                     /locus_tag="VNZ06_00760"
     CDS             complement(5996..7522)
                     /gene="ppx"
                     /locus_tag="VNZ06_00760"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR03706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HWW19307.1"
                     /translation="MNRSAAAMFEVIAAVDLGSNSFHMVVARYSHGQLVIIDRLREMV
                     RLASRISDDGRLDKDAAARALACLERFGQRLRDMNARNVRVVGTNALRVARRKQAFLE
                     RAREALGFPIEIVSGMEEARLIYSGVSHNLPNEPGRRLVTDIGGGSTEIIIGEQFEPL
                     EMESLQIGCVSLSQRYFSEGRLSARRIERARLAARLELEPIQAAFLRRGWDRAFGSSG
                     TVRAISESIRQLDPTASSISPAGLELLLQRLADTGHTRELDLDAVTDERRPVFPGGVV
                     IMAEIIDALGIQQLRFVEGALRDGLLHDMVGRLTRSDARERTVGSMQRRYHVDLGQAE
                     RVESAALQFLAQVRQSWGLTDPLAEQSLRWAGRLHEIGLDVAHNGYHRHGAYLLDNAD
                     MPGFSREEQQLLARLVGSHRRKLTLERLEDLIPPWDGLALFLILLLRLAVLLQRGRSA
                     VALPHIELNVRGRTLELRFPAKWLKEHPLTVADLQQEIAYLRAQSIRLRVFSGTEPVN
                     "
     gene            complement(7564..7932)
                     /gene="erpA"
                     /locus_tag="VNZ06_00765"
     CDS             complement(7564..7932)
                     /gene="erpA"
                     /locus_tag="VNZ06_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009408529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HWW19308.1"
                     /translation="MTEIAELTAPESVESPLLFTDAAARKVSELIAGEGNPKLMLRVF
                     VQGGGCSGLQYGFEFDEEMQDGDTCVENQGVKLLIDPMSFQYLGGAEIDFRDGLDGAQ
                     FVIRNPNAQTTCGCGSSFSA"
     gene            8020..8856
                     /gene="panB"
                     /locus_tag="VNZ06_00770"
     CDS             8020..8856
                     /gene="panB"
                     /locus_tag="VNZ06_00770"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWW19309.1"
                     /translation="MSTERSSGEGGPKIRRVTTARLTEMKARGEKIAMLTGYDYTFAR
                     ILDGAGIDVVLVGDSASNVVHGHETTLPITLDQMIDHANAVVRGVKRALVVVDMPFGS
                     YQGDSQLALQGAVRIMKESGAHAVKMEGGEKIVEGISRVLSAGIPVMGHLGLTPQSIN
                     RFGTYVVRAREKQEAEQLVRDAVLLEKTGCFAIVLEKVPAELAQQVAGKLTIPIIGIG
                     AGPNVDGQVLVTHDMLGLNNEFSPRFIRRYASLFDEVTDAFSRYIADVKAERFPTREE
                     SY"
     gene            complement(8853..9716)
                     /locus_tag="VNZ06_00775"
     CDS             complement(8853..9716)
                     /locus_tag="VNZ06_00775"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWW19310.1"
                     /translation="MSPDLLSVVVRTLGLVGLFQAAGAAFFLVLFGEWVPNSHAAIRR
                     LGWWAAIAGIVLLACHQGLEAARMSDAFSGLFDSRLEALAWGGSSGNAALLQILGLGV
                     VALGFALAPLTGSQMAGVGGCIAACAAVLTGHTSVHSERPLLGLLLAVHLMLVAFWFG
                     ALLPLMFVCGRESKSAAVALLNGFSKIAGLLVPCIAVAGLTMALVLMPDRMSWISAYG
                     WLILAKIAAFAVLLLLAAWNRWRAVPALAADSSETSGSVLRRVMGVEYLLIVAVLAMT
                     AVLTSFYSPSS"
     gene            complement(9726..10079)
                     /locus_tag="VNZ06_00780"
     CDS             complement(9726..10079)
                     /locus_tag="VNZ06_00780"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HWW19311.1"
                     /translation="MKRLAGVFGVWALLAAGAVYAHAHLLQATPADGSVLTQAPTAFA
                     LKFNEAATLTALTLQKGAQAAQKIEGLPTKPSALFSIPAPKLDAGDYTLNFRVLSDDN
                     HVVSGSIKFKVNADK"
     gene            complement(10364..10681)
                     /locus_tag="VNZ06_00785"
     CDS             complement(10364..10681)
                     /locus_tag="VNZ06_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19312.1"
                     /translation="MRSLCKPLCVIALLLCKLAQAALVPIPTELNTCPRHSMQQRMVG
                     SESMPHASCCKFSGCDCLQAPALAGPGLSAAVFAGTADCASVLHSDQPRRLAGWFFRP
                     PIA"
     gene            complement(10678..11058)
                     /locus_tag="VNZ06_00790"
     CDS             complement(10678..11058)
                     /locus_tag="VNZ06_00790"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWW19313.1"
                     /translation="MFKKREKDGIGPIDTLIGKNASVQGHIEFFGGLHIDGRVIGNVT
                     AASGADASLSVSEEGVIEGSVEARNVVLNGRVNGDILASERVMLGAKARVNGDVHYGV
                     IEMAHGAEISGKLVPRNGVAVLPK"
     gene            complement(11070..11798)
                     /locus_tag="VNZ06_00795"
     CDS             complement(11070..11798)
                     /locus_tag="VNZ06_00795"
                     /inference="COORDINATES: protein motif:HMM:NF042896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HWW19314.1"
                     /translation="MEIPGKLVVRTYAPARRWIMLAIVLLLGSLALYVVFELGRFTAG
                     YDAMQAAAERDALQLQINQQQQSMREMRVQLAAAEEARVSEVRERSEVARTIGELQAQ
                     VERQQQDVEFYRGLVAQPNQAKDAVLVGVQQFHIASLQGNQRFALRFSLNRQQRPGEP
                     LSGVLGITVDGTKDGVPVSASLADLTGAKNELLFSVRYTTSIDQEVILPGDFKPERVT
                     IELRPDKKGISPYRQTFVWRVDPS"
     gene            complement(11889..12356)
                     /locus_tag="VNZ06_00800"
                     /pseudo
     CDS             complement(11889..12356)
                     /locus_tag="VNZ06_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016144233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
BASE COUNT         2452 a         4093 c         3709 g         2332 t
ORIGIN      
        1 agcgcgttag ctacgacact gggaggctta ccctctcaac gtctagttct catcgtttat
       61 ggcgtggact accagggtat ctaatcctgt ttgctcccca cgctttcgtg cctgaacgtc
      121 ggtgctggac caggaagccg ccttcgccac tgatgttcct tccgatatct acgcatttca
      181 ccgctacacc ggaaattccg cttccctctt ccgcacccaa gccttgcagt ttcgaatgca
      241 attcccaggt tgagcccggg gatttcacat ccgacttaca aaaccgtcta cgcacgcttt
      301 acgcccagta attccgatta acgctcgcac cctctgtatt accgcggctg ctggcacaga
      361 gttagccggt gctttttctt tgggttaggt caagacccag aggtgttagc tctgagcttt
      421 tcatccccac taaaagtgct ttacaacccg caggccttct tcacacacgc ggcgtcgctg
      481 gatcagggtt gcccccattg tccaatattc cctactgctg cctcccgtag gagtctgggc
      541 cgtgtctcag tcccagtgtg gctggtcgtc ctctcagacc agctacggat tgtcgccttg
      601 gtaggccgtt accctaccaa ctagctaatc cgacttcggc tcatctaata gcgcgaggtc
      661 ttgcgatccc ccgctttcaa ccgtagttcg tatgcggcat tagctaacct ttcggttagt
      721 tattccccac tactaggcag attccgaagc gttactcacc cgttcgccgc tcgtcagcat
      781 gtattgctac acctgttacc gctcgacttg catgtattag gcacgccgcc agcgttcaat
      841 ctgagccagg atcaaactct tcacttaaat tttttgagct tcaagtgatg agtgaatcca
      901 aatgcccatc gctggacttt tggtcactcg gtcaatgaca tccaaaggac gtatcgacgc
      961 gaggtcccac acaagttacc taccgaattt ttaaacagca gctgagccgc ttgggcacag
     1021 caatccacgg ccatacggcc gcagaaaggg tcgggaagta tagccctcgg gctcttcccg
     1081 tcaacataac agtcaacgtg ttttccaatt ggcccgcgct agcgcgagag cctcaggtag
     1141 gaccttggct gagggcgcgc attatagggg tagaaacccc tatgtcaacg agttgtcatc
     1201 actttattta gcggccgaca agcctatgaa ggggcttgct ttttttcgat caaaatataa
     1261 gcaaagcggt cgcggaccgc gctgcaggat acgaccggtc cgcaagcgac gatcgcgttg
     1321 cagcatccct tcccctctcc cctcgatcgc atggtcgagc atgcgtgacc tgctgcgttg
     1381 acccgtggct taaggccacg gtagggtcgg tcacatgagt accggaacgg acttaccagc
     1441 cgacacgctc ggaagtccct ggctgctgcg atcactgcag ctgctggcct gttttgcagt
     1501 catttcctac accctgcagg tcgcccgcca cactacgccg ccaacgctgc aactgggcac
     1561 catgctgttt ggcgccagcg atcatgcgcc ggggaacccg gtcgatgtga acgacatctc
     1621 cgctgtgctg gcacccggcc tggatctggc cgaagtcatc gttcagcgca atgacaccct
     1681 ggatcacatc ttccgtcgcc tgcagttgag cctttccgat ctcgcggacg tgcgcgcact
     1741 cagtggcgtt cagaccatgc tcgaccgact cagtcccggg gagcatctga aattcctcca
     1801 tcgcgacgcg gcgctgatcg gtctcgagcg ccacctgtcg ctgaccgaaa aactggaagt
     1861 caggcgcacg gacgccggct ttcgggcaac cgtcgtgccc aaggcgatcc agatcgacga
     1921 cagcgtggcg cacggcgaga tcaactcctc actgttcgaa tccgccaatg ccgccggcct
     1981 gtcagacctc agcgtgctca agctggcaaa gatcttcgca tcggatatcg atttcgtgat
     2041 tggtctgcgc gagggcgatc gcttcgtcgt cagctacgag cgaatccttc agaacgacca
     2101 ctacgtaaag gacggcgaca tcctggccgc ccgcttcatc aatcagggcc gggtatatga
     2161 ggcggtgcgt tacgtcggac ctgacggcgc ggcacgctac tactcgcccg atggccacag
     2221 taccgagaag gctttcctgc gcgcaccgct ggaattcaag cgcatcagct ccggattctc
     2281 gctcgcgcgc atgcatccga tcctcaacct gatccgcgcc cacaagggca tcgactacgc
     2341 cgcgccgatt ggcaccccgg tgtatgccgc aggctccggc cacatcaagt tccgtggcga
     2401 tctgggcggc tacggcaacg tcatccagat cgagcacggc aacggcatcg tgacggtgta
     2461 cggccacatg tcgagattcg ctggcgcgcg cgccggcgcg cacgtggagc gcggcgagac
     2521 catcggctac gtcggcatga cgggactggc gaccggtccg catcttcatt ttgaatttcg
     2581 cgtgaacggc caatatgtgg atccgcagaa gatcaagctt ccggacgcga agccgatcga
     2641 tccggatctc tacgatgact tcaaacaaaa aagcgcaccc ctgctggcag cactgaatcc
     2701 atgagtcttt atgtcgggct gatgtccggt accagcatgg acggtatcga tgctgtgctg
     2761 ctgcagatcg atgaaggcgg catgcgcgtc aaagccgccg tcgcccggca ctggccggcc
     2821 gatctgcagc ggcgcctgcg tcaggccgcc gaggactatc tgagtctggg attgctcgaa
     2881 tttggacggc tggacacgat cgtcggccgc gaatttgcca gcgcggccga ggaactgctc
     2941 aacaggagcg gcaccgcact gtccgcggtg cgcgcgatcg gcagccacgg tcagaccgta
     3001 ttgcatcagc caggggatgc cgcaccgttc acgctgcaga tcggcgatcc caacatcatc
     3061 gccgaacgcc tcaatatcga tgtcgtcgcg gactttcgcc ggcgcgacct cgccgccggt
     3121 ggcgaaggtg cgcccctgat gccggcgttt cacgtcgcgg cgttcggcac accgggccag
     3181 tacaacgccg tagcgaatat cggcggcatt gcgaacgtct cgcggctaca ggccgacggc
     3241 agcgtcaccg ggtttgatac cgggcctggg aactgtctgc tggatgcctg gtcacgccag
     3301 cagcgagcca agcccttcga cgagcacggc cagtgggcag ccagtggtgt cattcagccg
     3361 acgctgctca accgcatgct ccaggatccc tactttgcgc gccgcccgcc caagagcacg
     3421 ggccgcgaga ccttcagtga cggctggctc gagcaggcgc tggccggcta ccgggcatcg
     3481 ccggccgatg tacaggcaac cctcacggaa ctcaccgcgc acacgattgc catgagcctg
     3541 gccggaggcg gcacccagcc ggtgcgcgta ctggtctgtg gcggcggcgc tcagaacacc
     3601 tatttgatga gccggctaag tctcgcgctg ccgcgctcgc aagtgcagac cacgggcgcg
     3661 tgcggcatcg accctcagca cgtcgaagcc gccggattcg cctggctcgc ctaccgctat
     3721 gtcaacgcac tgccgggcaa tctgccgagc gtcaccggcg caaagtacgc tgtgccgctc
     3781 ggtgcgcttt atcgcggttc agcgaagtaa cagactcgtg caacaatgcc cctatggatg
     3841 caccggccaa agcagcacgc agggtatcaa agccagctcc ggcacccgca gcagcgataa
     3901 ccgagcagaa gactgccatg gatcaaccgg tatttcgtgc accgcagaat ttcatcaatc
     3961 gcgaattgtc gtcgctcgag ttcaatcagc gggtactggc ccagagcctg gacttgcgcg
     4021 tgccactgct ggagcggctg cgatttctgt gcatctcgtg cgccaatctg gatgagttct
     4081 tcgaaatccg tgtcgcggga ctgaagcaac tgctcgagtt gggttccgcc cagctcggcc
     4141 ccgacggttt gggtattgca gagcaactgt ccatgattca cgagcgcacc acccgcctgg
     4201 tgcacgatca atacgcctgc ctcaacgatg tgctgttgcc ggcgctgtcg gcggccaaga
     4261 tcgcggtgct cgatgaggcc gactgggatg acaccacgcg tgtgtgggtc gagaagcact
     4321 ttctggccga agtactgccg gtgttaagcc cgctgggtct ggatcccgcg cgcccgttcc
     4381 cgcgcattca gaacaagagc ctcaatttca tcgtgcgact gtccggcaag gacgcttacg
     4441 gacgcgacag cgaactggcg atcgtgcagg cgccccgatc cctgccgcgc atcgtgccgc
     4501 tgccggacga gggcccgacg cgccgtttcg ttgcgctgtc caccatcgtc gaggcattcg
     4561 tcgcgcacct gttcgaggga gttcaggtcc tcggctgcta tcagttccgg ctcacccgaa
     4621 acagcgacct gtttgtcgac gacgaagaag tggatgacct gcgccgcgcg ctcgaaggcg
     4681 aactcgcgca acgccgttac ggcgccgcgg tgcggctgga aactgccgcc aactgtccag
     4741 ccgacaccgt ggaatttctg ctgcgcgaat tcgcactcag ttcgcgggat tgttatcagg
     4801 taccggggcc ggtgaacctg aatcgcttca gcgccatcta tgacctggtg cagcgtaccg
     4861 acctgaaata tccgacgttc acgcccggct tgccggcgcg cctggtcggc gccacggacc
     4921 tgttcgcagc catccgccag cgcgacctgc tgctgcatca tccgtatcgc agcttcgggc
     4981 cggtgatgga tctgctgcgg caagccgcga gcgatcccgc cgtgctcgcc attaagcaga
     5041 ctttgtatcg cacgggtgat cactcgccga ttgtcgacgc actggtagcc gccgcgatgg
     5101 ccgacaagga cgtcaccgtc atcatcgaac tgcgggcgcg cttcgacgag gaagccaaca
     5161 tcgagctggc gacgcgtctg caggaagccg gcgcgcacgt gatgtacggc gtggtcggct
     5221 acaagaccca cgccaagatg acactgatcg tgcggcgcga gaccgacggc atgcgccgct
     5281 actgccacct cggaacgggc aactatcatc ccaagaccgc gcgtggctat accgactacg
     5341 gcctgttcac atgcgatgag gccatcggct ccgacgtgca cgagattttc ctccagctga
     5401 ccagcctcac acgcactccg aagctgcgcc ggctgctgca atcgccgttc catctgcatg
     5461 aggcgacgct ggcgctgatc gaacgtgaga gcgcgcatgc gctggcgggc aagaaagcgc
     5521 ggatcatcgt caagctcaat gccctggtcg atccgcaaac gatcgagacg ctgtaccgcg
     5581 cctcgcgcag cggggtgaag atcgatctga tcgtgcgcgg tgtttgtgcc gtgcgtcccg
     5641 ggatcccggg cgtgtccgag aacatccgcg tgatttcagt ggtcggcagg ttcctcgagc
     5701 actcacgtgt ctactggctc gcgaatgacg gctcgccgga gctgtacctg tcgagcgcgg
     5761 actggatgga gcgcaacttc ttccggcgcg ttgaagtcgc attcccggtg ctgcagccgc
     5821 agcaccgcga acgcatcgtg aaggacctgg agacttatct gaaggacaac accaacgcgt
     5881 gggagctgca ggccgatggc acttacgcac gcgtcaatgt cagcagcgca ccgaccgtgg
     5941 actcacaggc attgctgctc gagcgatatg ccgggacgcc tgcgccgccg gcgccctagt
     6001 tgaccggctc agttcccgag aagacgcgca ggcggatgct ctgtgcgcgc aagtaggcga
     6061 tctcctgctg cagatcggcc accgtgagcg gatgttcctt gagccacttg gcggggaagc
     6121 gcagttccag cgtgcgccca cgaacattga gttcaatgtg aggcagcgcg accgcgctcc
     6181 gaccacgctg cagcagcacc gccagtcgca gcagcagaat cagaaacagt gccagtccgt
     6241 cccagggtgg gatcaggtcc tccagtctct cgagtgtcag tttgcgacga tggctgccga
     6301 ccagacgtgc gagcagctgc tgctcttcgc gcgagaatcc tggcatgtcg gcgttgtcga
     6361 gcaggtaggc accgtggcga tgatacccat tgtgcgcaac gtccagtccg atttcgtgca
     6421 ggcggccggc ccatcgtaaa ctctgctccg cgagtggatc ggtcaatccc caggactggc
     6481 ggacctgggc gagaaactgc agtgcggcac tctccacgcg ctcggcctgt ccaagatcga
     6541 cgtgatagcg ccgctgcatc gagccgacgg tccgctcacg cgcatccgag cgcgtcagtc
     6601 ggccgaccat gtcgtgcagt agcccgtcgc gcaacgcacc ctcgacgaag cgcagctgct
     6661 ggatgcccag cgcgtcgatg atctccgcca tgatgacgac gccgcccgga aacaccggcc
     6721 gacgctcatc cgtgacggca tccagatcga gctcacgcgt gtgcccggtg tccgccagtc
     6781 gctgtagcaa cagctcgagc cccgcggggg aaatgctgct cgccgtggga tcgagctggc
     6841 ggatgctctc ggaaatcgcg cgtaccgttc cggagctgcc gaaggcccga tcccagccac
     6901 ggcgcaggaa ggccgcctga atgggctcga gttccagtcg cgccgcgagc cgcgcccgtt
     6961 cgatgcgtcg ggccgacagc cgtccctcgc tgaagtagcg ctgactgagc gatacgcagc
     7021 cgatctgcag gctttccatc tccagcggct cgaactgctc gccgatgatg atctcggtac
     7081 tgccgccacc gatgtcggtg accagacgcc ggccaggctc gttcggcaga ttgtgcgaaa
     7141 cgcccgaata aatgaggcgg gcttcctcca taccggacac gatttcgatc gggaagccca
     7201 gcgcttcgcg cgcccgctcc agaaacgcct gcttgcgacg cgccacacgc agtgcgttgg
     7261 ttcctaccac acgcacgttg cgcgcgttca tgtcgcgcag gcgttgcccg aatcgctcca
     7321 gacatgccag ggcgcgtgcc gcggcatcct tgtcgagccg gccatcgtcg ctgatgcgcg
     7381 aggcgagtcg caccatctcg cgcaagcgat cgatgatgac gagctggccg tgcgaatagc
     7441 gcgccaccac catgtggaag ctgttggaac ccaggtcaac cgccgcaatg acctcgaaca
     7501 tcgcggcggc actgcgattc atggcgtcaa cggacccagg ctaggcccgg ggcagagcac
     7561 agactaggcg ctgaaggagg aaccacatcc gcaggtggtc tgagcgtttg gattgcggat
     7621 cacaaactgt gcgccatcga gtccatcgcg gaaatcgatc tcagcgccgc ccaggtattg
     7681 aaaactcatc ggatcgatca gcagcttcac accctggttt tcgacgcagg tgtcgccgtc
     7741 ttgcatttcc tcatcgaact cgaaaccgta ttgcaggccc gaacagccgc cgccctgaac
     7801 gaatacgcgc agcatcagct tgggattacc ctcgccggcg atcagttcgc tcaccttgcg
     7861 tgccgccgcg tcggtgaaca acagcggtga ctcgacggat tcaggtgcag tcagttcagc
     7921 tatttctgtc atatacaaga gtttaggcgt gtgcgccagg tacgtcaaag cgggctatag
     7981 tgacactcat gacggttata tgaagtttgc gggggtgata tgtcaaccga gcgatcgtcc
     8041 ggcgaaggcg ggccgaaaat tcgtcgagtc acgacagcgc gcctgacgga gatgaaagcg
     8101 cgcggcgaga agatcgcgat gctgacaggc tatgattaca cgttcgcccg catcctcgac
     8161 ggagccggca tcgacgtggt gctggtcggt gacagtgcat cgaacgtggt gcatggccac
     8221 gagaccacgt tgcctatcac gctcgatcag atgatcgatc atgccaacgc ggtggtccgg
     8281 ggcgtgaagc gcgcgctggt ggtcgtggac atgccctttg gcagctatca gggagacagc
     8341 cagctcgcgc tgcagggcgc cgtgcgcatc atgaaggagt cgggcgcgca cgcggtcaaa
     8401 atggaaggcg gcgagaagat cgttgaaggc atcagccgcg tgctgtccgc cggcattccg
     8461 gtgatgggcc atctgggcct gacgccgcag tcaatcaatc gattcggcac ctacgtcgtt
     8521 cgcgcacgcg agaagcagga agccgaacag ctggtacgcg atgcggtgct gctggagaag
     8581 accggctgct tcgcgatcgt cctggaaaag gtaccggcag agctggcgca gcaggtggct
     8641 ggcaagctga ccattccgat catcggcatc ggcgcgggcc cgaacgtgga cgggcaggtg
     8701 ctggtgacgc acgacatgct gggattgaac aacgaattct caccgcgttt catccgacgg
     8761 tacgcctcgt tgttcgacga agtcaccgac gccttctcgc gttatatcgc cgacgtcaag
     8821 gccgagcgct tcccgacgcg cgaagagagc tactagctac tgggggagta aaagctcgtc
     8881 agtaccgcgg tcatcgccag caccgcgacg atcagcaggt attcaacgcc catcacccga
     8941 cgcaggacac tacctgaggt ctcggaactg tcggcggcga gtgccggcac ggcgcgccac
     9001 cgattccacg cggccagcag cagcagcact gcaaatgcgg cgattttcgc caatatcagc
     9061 caaccatacg cgcttatcca cgacattcgg tccggcatca ggaccagcgc catggtcaga
     9121 ccggccacag caatgcacgg gaccagtagt ccggcgatct tcgagaaccc gttcagcagc
     9181 gccacagccg ctgatttcga ttcacggcca cacacgaaca tcagcggtag cagtgctccg
     9241 aaccagaaag ccaccagcat gaggtgaacc gccagcagca gtccgagcaa tggacgttcc
     9301 gaatggacac ttgtgtgacc ggtcagcacc gcggcacagg ccgcgatgca accgccgacg
     9361 ccggccatct gcgatccggt caagggcgcc agagcgaacc ccagagcaac gacaccgaga
     9421 cccagaatct gcagcaacgc cgcattgccg ctgctgccgc cccaggccag agcctccagg
     9481 cgcgaatcga acagacccga gaaagcgtca ctcatgcgtg cggcctcgag cccctgatga
     9541 caggccaaca gcacgatacc ggcgatagcc gcccaccaac ccagtcgtcg gatcgccgcg
     9601 tgagaattcg gcacccactc accgaacaag accagaaaga acgcggcgcc cgcggcctga
     9661 aacaggccga ccaagcccaa tgtccgcacc accaccgata gcaggtccgg actcacggcc
     9721 gcggcttact tgtccgcgtt gaccttgaac ttgatgctgc cggagaccac gtggttgtcg
     9781 tcactgagca cgcggaagtt caacgtgtag tcgcccgcat cgagcttggg tgccggaatg
     9841 ctgaacagcg cggacggctt ggttggcaga ccttcgatct tctgcgcggc ctgagctccc
     9901 ttctgaagcg tcagcgcggt cagcgttgcc gcctcgttga acttcagcgc gaacgccgtg
     9961 ggagcctgtg tcagcacgct gccatcggcc ggcgttgcct gcaacaggtg tgcatgggcg
    10021 tacaccgcgc cagcggccag cagcgcccag accccgaata ctcctgccag tcgtttcata
    10081 cctagctcct cctgtattgg tcaaagtagc atcctgattc cggcgctcca ttgcaatgcg
    10141 cccggaacct ggccggcgtc gctcaaccac tgggcggtgg caccgaatcg acgggtccag
    10201 accagcgcac tgctgctcag ccctcgtacg aacgtacgcc gggtgctctt catcttgaga
    10261 atctccaatc ctcaacatcg cgcagacgcg cgacccgcgt acaggcagga gcgcagtctg
    10321 cgacgtgcac ttgcgcctga cgtgctgtgc taagcgagga gagttaagcg atgggaggtc
    10381 ggaagaacca gcccgccagg cgccgcggct gatccgaatg caacaccgag gcgcagtccg
    10441 cggtgccggc gaaaaccgct gccgacaacc cagggcccgc taacgcaggg gcctgcaggc
    10501 agtcgcagcc ggagaacttg caacagctcg cgtgcggcat ggattcgctg ccgaccatcc
    10561 gctgctgcat ggagtgacgt ggacaggtat tgagttctgt cggtattggc accagcgccg
    10621 cctgcgccag cttgcatagc agcagcgcga tcacgcacag cggcttgcac agcgatctca
    10681 cttggggagt accgccacgc cattgcgcgg caccagcttg ccgctgatct ccgcgccatg
    10741 cgccatttcg atcacaccgt agtgcacgtc gccattgaca cgcgccttcg cacccagcat
    10801 gacgcgctcg cttgccagga tgtctccgtt gacccgccca ttgagcacca cgttgcgcgc
    10861 ttccaccgac ccttcgatga caccctcttc gctaacggac agcgatgcat cggcgccgga
    10921 ggctgccgtg acgttgccga tcacgcggcc atcgatgtgc agcccgccga aaaactcgat
    10981 atggccctgc acgctggcgt tcttcccgat cagcgtatcg atggggccga tgccgtcctt
    11041 ttcacgtttc ttgaacatga actcctgcgt cagctcggat cgaccctcca gacgaaagtt
    11101 tgccggtaag gcgagatccc tttcttgtct ggacgcagct caatggtgac tctttcaggc
    11161 ttgaagtcgc ctgggagtat aacttcctga tcgatactgg tggtgtaacg aacactgaac
    11221 agcagctcat tcttggcccc ggtaagatcc gccaggctcg cgctgacggg aacgccgtcc
    11281 ttggtgccat cgaccgtaat gccgagcaca ccgctcagtg gctcgcccgg acgctgctgg
    11341 cgattcagac tgaagcgcag tgcaaatctc tgattgccct gcagcgaagc gatatgaaac
    11401 tgctgcaccc cgaccaagac cgcgtcttta gcctgattgg gctgcgcgac cagcccgcga
    11461 tagaactcga cgtcctgttg ttggcgctca acctgcgcct gaagttcccc aatcgtgcgc
    11521 gccacttccg aacgctcgcg aacctcggag acccgcgctt cctccgccgc ggcgagttgc
    11581 acgcgcattt cgcgcataga ctgctgctgc tgattgatct gcagctgcag cgcgtcgcgc
    11641 tcagccgcgg cctgcatcgc atcgtaaccc gcggtgaagc gccccaattc gaacaccacg
    11701 tacaacgcca ggctaccgag cagcaacaca atcgccagca tgatccagcg gcgcgccggc
    11761 gcataggttc ttactaccag cttgccggga atttccatcg cgttcgggcg cagctattgc
    11821 gccacttgtt ccgcggtcat ggccttggcg cgccggcgcc accacgccgg atagggcggc
    11881 agaccgccgg gaatcatcgt tcccagctca tgcagcgccc gcgaatacac tgaacgcttg
    11941 aaatagacca cctcgcggat cggctgccag taatccaccc agcgccaacg gtcgaattcg
    12001 ggttgtgagg tggtatcgaa gcgaaacggt acgtgctcgc gcttcagctg cagcagaaac
    12061 caatactgct tctgccccac gcacagcggc tgtacatttc gccgcacgta gcgcgcgggc
    12121 aatcgataac gcagccaata gcgcgtgcgg ccaatgacag ccacgtcatt gcggctcagg
    12181 ccaatttcct cccgcaactc gcgataaagg gattcctcat cgctctcccc acggcacatg
    12241 ccgccctggg gaaactgcca gccacgcccg ccggcgcgac ggccgatgaa aagctgtccc
    12301 tccccggaca tcagcacgat gccgacattg gcgcggaaac cgtcgcgatc gatgatgtca
    12361 cccatggcac acttttaccc ccgcagcgcg ggacgctcaa gctaccctag agaatgatgc
    12421 gaacccagta ctggtggggc ctggccacct taagccttct ggccgtcatc gccttctgcg
    12481 ccgagctatt gatcggttcg gccggtttgt cctcgggccg ggcactgcag gcgctgttcg
    12541 ggcacggcga caatttcagt ctaatgatcg tgcagaccgt gcggct
//
LOCUS       DATMRM010000028         2444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4352640, whole genome shotgun sequence.
ACCESSION   DATMRM010000028 DATMRM010000000
VERSION     DATMRM010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4352640"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="VNZ06_00805"
     CDS             complement(<1..1315)
                     /locus_tag="VNZ06_00805"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HWW19315.1"
                     /translation="MSNQVVGSRLLGAIAFVTLFAACTPQPSTAAPPASDAPRKAPLV
                     PAAAVTPSPSATAAAARGLPDFADLVAQVGPAVVNVAVVEKSPSAQNSDEDEDSDDPF
                     SDFFRRFGLPRPNSPSPRNNAPVRGIGSGFIISPDGYILTNTHVVNNASKITVKLTDR
                     REFEGKVVGSDEKTDVAVIKINAKGELPVVRLGDSSRLRPGQWVLAIGSPFGFENSVT
                     AGVVSALARGNVGEAGNGYVPFIQTDVAVNPGNSGGPLFNLSGEVVGINSQIYSRSGG
                     YEGISFAIPIDVANNVAEQLIKSGHVSRGRIGVTILEVSAATAENLGLDRPRGAAVAS
                     VESGGPADKAGIEPLDIILSVNGRGVETSDQLPLMISEIKAGQSAQLEVWRDKAIKKI
                     SVTVEELKEKTTEARARRFNNSGGSEESDSTTVSRIGLTVRTLTSQ"
     gene            complement(1384..>2444)
                     /gene="lnt"
                     /locus_tag="VNZ06_00810"
     CDS             complement(1384..>2444)
                     /gene="lnt"
                     /locus_tag="VNZ06_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HWW19316.1"
                     /translation="WLALGYTQTDTWLSGFAGVFGIYGISALILLGSGALVALVRGKG
                     RVRWAACLVLLLPWPLGLALKQIQWTHPSGPAITVAVLQGAVPENIKWESENVEPTRA
                     LYTQLNEQALGARLIVWPEAALPQIANELVPYLKQLYQRSTQQGSDVVMGILRADEND
                     DYHNSIMVLGSDVSFYDKHHLVPFAEFFPVPSFVRSWLRLKNLPYSDFTPGRAAQASV
                     AAAGTRLALGICYEDAYGTNDLPAIRDSGVLVNVTNDAWFGHSWARFQHFQIARMRTL
                     EAQRPLVRAANDGISALVGPQGEVLAQAEQFKPAVLRGSVQPRAGLSPFLRFGNWPIV
                     VLGLLGAGLAAGLKRRYR"
BASE COUNT          475 a          791 c          742 g          436 t
ORIGIN      
        1 cctgactggt gagcgtgcgc accgtcagac cgatccggct gaccgtggtt gaatcgctct
       61 cctcgctgcc gccgctattg ttaaagcggc gggcccgggc ttcggtcgtc ttctctttga
      121 gttcctcgac ggtcaccgag atcttcttga ttgccttgtc gcgccacact tccagttgcg
      181 cgctctgccc ggccttgatc tcggagatca tcagcggtaa ctgatcggaa gtctctacgc
      241 cgcggccgtt gacgctcagg ataatgtcca gcggctcgat accggccttg tccgccgggc
      301 cgcccgactc aaccgaggcg actgcggcac cgcgcggacg gtcaagcccc aggttctcgg
      361 cggtcgccgc actgacttcc agaatcgtga cgccgatacg gccgcggctc acatggccgg
      421 atttgatgag ctgttcggca acgttgttcg caacgtcgat cgggatcgcg aacgagatac
      481 cctcatagcc accgctgcgg ctgtagatct gtgagttgat gccgaccact tcgccactga
      541 gattgaacaa cggtccgccg gaattgccgg gattgactgc cacgtcggtc tggatgaagg
      601 gcacatagcc gttgccagcc tccccgacat tgccgcgcgc cagcgcactg accacgccgg
      661 ccgtgacgct attctcgaag ccaaatggcg agccgatcgc cagtacccac tggcccggtc
      721 gcagcctgct ggaatcgccg agtcgcacca ccggcagttc acctttggcg ttgatcttga
      781 tgacggcaac gtcagtcttc tcgtcgctgc cgacaacctt gccttcgaac tcacgccgat
      841 cggtcagctt cacagtgatc ttggaagcgt tgttcaccac gtgcgtgttg gtgaggatgt
      901 agccgtccgg gctgatgatg aatccggagc cgatcccgcg cacgggtgca ttgtttctag
      961 gcgacgggct gttcggccgc ggcaagccga accggcggaa gaagtcgctg aacggatcat
     1021 ccgaatcctc atcttcatcg gagttctggg cggaagggga cttctctacc accgccacgt
     1081 tgaccaccgc gggaccgacc tgcgccacca gatcggcgaa atccggcagc ccgcgcgcag
     1141 cggcggcggt tgcagagggc gacggcgtca ccgcggcggc cggaaccagc ggcgccttgc
     1201 gtggcgcatc gcttgccggg ggtgccgcgg tcgagggctg cggcgtgcag gccgcaaaca
     1261 gcgtgacgaa agcgatggcg cccaacagac gcgacccaac cacctgattc gacatatgag
     1321 gtctactcca agaaacaacc agaaacggat gaataataga ccgggattcc cgatcgcggg
     1381 ttcctagcgg taccgacgtt tgaggccggc agccaggccg gcccccagta gtcccaatac
     1441 tacgataggc caattgccaa aacgcagaaa aggggacaga cccgcgcgcg gttgcacgct
     1501 gccgcgcagt accgcgggtt tgaactgctc agcctgcgcc aggacttcac cctgcggtcc
     1561 aaccagcgcc gagataccgt cgttcgccgc ccgaaccaac ggcctctgag cctcgagcgt
     1621 gcgcatacga gcgatctgaa agtgctgaaa acgggcccat gagtgaccga accaggcatc
     1681 gttggtcaca ttcaccagta ctccggaatc gcggatcgcc ggtagatcgt tagtgccata
     1741 ggcatcctcg taacagattc ccagtgccag gcgcgtgccg gcggcggcca ctgatgcttg
     1801 agcggcgcgc ccgggcgtaa aatccgaata cggcaggttc ttcaatcgaa gccacgaacg
     1861 cacgaacgag ggtaccggga agaactcggc gaaaggcacc aggtgatgct tgtcgtagaa
     1921 gctgacatcg ctgccgagca ccatgatcga gttatggtag tcgtcgttct cgtccgcccg
     1981 caggattccc atcaccacat ctgaaccctg ctgcgtcgag cgctgataga gctgtttgag
     2041 atagggcacc agctcgttgg cgatttgtgg cagcgcggcc tcgggccaga cgatcagtcg
     2101 tgcgccaagg gcctgctcgt tgagctgcgt atacagcgca cgcgtgggct cgacgttctc
     2161 gctctcccat ttgatgttct ccggcaccgc tccctgcagc accgcgaccg tgatggcagg
     2221 cccggacgga tgagtccact gaatctgctt caaagccaga cccagcggcc agggcaacag
     2281 caacaccagg caggcagccc agcgaacacg tcccttgccg cgcaccagtg ctaccagcgc
     2341 accgcttccc agcagaatga gcgcggagat gccgtagatc ccgaacacgc cggcgaaccc
     2401 ggacagccag gtatcggttt gcgtgtagcc gagcgccagc cagg
//
LOCUS       DATMRM010000029         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4352723, whole genome shotgun sequence.
ACCESSION   DATMRM010000029 DATMRM010000000
VERSION     DATMRM010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4352723"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VNZ06_00815"
     CDS             complement(<1..259)
                     /locus_tag="VNZ06_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19317.1"
                     /translation="MPQAEQSGTRSRSRWPRIAARALLSCVALMWVLHTTPLRAIGSA
                     LTAAHLPLLLAGLLLNFCARLAAAERTLAISRGLGLWISRWQ"
     gene            426..1646
                     /locus_tag="VNZ06_00820"
     CDS             426..1646
                     /locus_tag="VNZ06_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWW19318.1"
                     /translation="MDVQAGRDRPRVLIVTPQPFYEDRGTPIAVQYVARALAEIGVDV
                     DLLAFPIGREVKIKNVTLKRCANLLHIRRVPIGFSWKKLVLDASLWTSFSKLLALRRY
                     DMVHAVEEAAYIAAAICPRFGQPFIYDMASAIPVELQRQPLLKSRRAQRFLRSMERWV
                     FHRATRVVCSPGLGRYVHNQAPDAPVTEWRFPAQLPHVPRQETESLRERLQIAPDRRI
                     VLYSGSFAGYQGIDLLFEAFVQARRSNPELLLICVGATEPEMAAWSKRTPKELVGHVR
                     ILPRQPRERIAAYIELADFLALPRGRTDNVPLKLFDYMASGKPIIAMRQAAYAPLLDQ
                     TRAFICDPTPEALAQAIGRACRSPEEAHLMGQASLRYAHRQFGWGRFVDFVRDTYSKS
                     IFTDREGSRLSRAS"
     gene            1739..>2576
                     /locus_tag="VNZ06_00825"
     CDS             1739..>2576
                     /locus_tag="VNZ06_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWW19319.1"
                     /translation="MNTRVRSAISRREAIRNALMLCVAGGGVERTWSAELPASNAPLS
                     RPIPSSGERIPIVGLGTDSFREDARDAIRAEIQRMHELGGTVIDTAAAYGPSEALIGE
                     ALVATGLRDRMFIATKLSATAAYDRINGEASFLRSLERLHAHIDLLQIHNLDGMDSLM
                     PLLQKWKQSGMIRYLGVTTSLNSQHAGIVEAMRRYPLDFIQVDYSIGNRDAATTVLPV
                     ALERKVAVLVNLPLERAALIGQASQRQLPDWVSSIDVKSWSQFFLKYVISHPAVTCAI
                     PGS"
BASE COUNT          469 a          786 c          801 g          520 t
ORIGIN      
        1 tctgccagcg cgagatccaa agtcccagcc cacgactgat cgccaacgtg cgctcggcag
       61 ctgccagtcg agcacagaaa ttgagcagca agccggccag cagcagcggc agatgcgctg
      121 ccgtcaacgc ggatccgatc gcgcgcagcg gtgtggtgtg cagcacccac atgagcgcaa
      181 cacagctcaa gagggcacgc gccgcgatcc tcggccagcg cgagcgcgag cgcgtgcctg
      241 actgttccgc ttggggcata gggctaaagt aacgaaatac cgccggccgc gcgattggcg
      301 cgcattccgt cccgcgttcg cgagtgccta caggctgaag ttacctcgtc ctactgcgtc
      361 gttgcggcgc gctcggcagg ctcagatgac ctgccgatct cttccctcgg tcaggagagg
      421 aatccatgga tgttcaagcc ggacgagatc ggccgcgtgt attgatcgtc acgccgcagc
      481 cattttacga ggatcgcggt acgccgatcg ccgttcagta cgtggcacgc gcgttggccg
      541 agatcggcgt ggacgtggat ttgttggcgt tcccgatcgg ccgcgaagtc aagatcaaga
      601 acgtgactct gaagcgctgc gccaacttgc tgcatatccg tcgcgtaccg atcggcttct
      661 cgtggaagaa actggtgctc gacgcgagtc tttggacttc attttcgaaa ctgctggccc
      721 tgcgccgcta cgacatggtg cacgcggtcg aagaggccgc gtatatcgct gccgcgattt
      781 gtccacggtt tggtcagccc ttcatctacg acatggcgtc ggctattccg gtcgagctgc
      841 aacgccagcc cctgctgaag tcgcggcgcg cacaacgctt tctgcgctcc atggagcgct
      901 gggtatttca ccgtgcgacg cgcgtggtgt gtagtccagg tctgggccga tacgtgcaca
      961 accaggcccc agacgcgcct gtaaccgagt ggcgctttcc tgcgcaattg ccgcacgtgc
     1021 ccaggcagga gactgaatcg ttgcgtgagc ggctgcagat tgctcccgat cgtcggatcg
     1081 tcctttattc tggcagcttt gccggctatc agggcatcga tctgctgttc gaggcattcg
     1141 tgcaggcgcg ccggagcaac cccgaactgc tgctcatctg tgtcggtgcg accgaaccgg
     1201 agatggcggc gtggtcaaaa cgcacaccca aagaattggt gggacacgta cggatattgc
     1261 cgcgtcagcc gcgcgagcga atcgctgcct acattgaact cgctgacttt cttgcgctgc
     1321 cccgagggcg gaccgacaac gtgccattga agctctttga ctatatggcg tcgggaaagc
     1381 cgatcattgc catgcgacag gccgcgtatg cgcccctgct tgatcagaca cgtgccttca
     1441 tttgcgatcc tacgcccgag gcgctcgcgc aggccattgg acgcgcctgc cgctcccccg
     1501 aggaagcaca cttgatgggc caggcgtcgc tgcggtatgc gcaccgccag ttcggctggg
     1561 gccgtttcgt tgatttcgtg cgtgatacct attcgaagtc catattcacc gatcgcgaag
     1621 gatcacggct gtcgcgggcg agctagcagg ggcggccgcg gctcttggct ggcgttttcg
     1681 gtcgcaaagt cgtataagcg cgcggctcgc ggctaagaat tgagtcatgg gggcttcgat
     1741 gaatacccga gttcgatcgg cgatctcgcg tcgcgaggcc attcgcaatg cactgatgct
     1801 gtgcgtagcc ggcggcgggg tcgagcgcac atggagcgcc gagcttcccg cctcgaacgc
     1861 gccgctgagc aggccgattc cgtccagtgg cgagcgcatc ccgatcgtgg gtctgggcac
     1921 cgactcgttt cgggaggatg cccgcgatgc tattcgagcc gagattcagc gcatgcacga
     1981 gctgggcgga actgttatcg atacggctgc cgcgtatggc cccagtgagg cattgatcgg
     2041 cgaagcgttg gtggctaccg gattgcgcga tcgtatgttc attgcgacca agctttccgc
     2101 cacagctgcc tatgacagga tcaatgggga ggccagtttt ctacgctcgc ttgaacgact
     2161 gcacgcccac atcgatctgc tccagatcca caacctcgat ggcatggaca gcctgatgcc
     2221 gctgctgcag aagtggaagc aatcgggaat gatccgttac ctcggcgtca cgacgtcgct
     2281 caattctcag cacgctggga tcgtcgaagc gatgcgcagg tatccattgg atttcatcca
     2341 ggtggactac tccatcggca atcgcgacgc cgcaaccacc gttcttccgg tagcgctgga
     2401 acgcaaagtg gccgtgttgg tgaatctgcc actggaacgc gccgcgctca ttggccaggc
     2461 ctcgcagcgt caactgccgg actgggtctc gagcatcgac gtgaagagct ggagtcagtt
     2521 tttcctaaag tacgtcatct cgcatccggc ggtcacctgt gcgattccgg gctcca
//
LOCUS       DATMRM010000030         7009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2490268, whole genome shotgun sequence.
ACCESSION   DATMRM010000030 DATMRM010000000
VERSION     DATMRM010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7009
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2490268"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VNZ06_00830"
     CDS             complement(<1..890)
                     /locus_tag="VNZ06_00830"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWW19320.1"
                     /translation="MRALLPFLVPHRAILFGALLALLVAAGAMLAAPAALRQLIDHGL
                     TTANTTILNRYLFGFLGASVAFGVFAALRFYLVTWAGERVVADLRQAVYRRVIRMDPL
                     FFESTRTGEVLSRLTADTTLVQALAGVNLSITLRSILSLVGGLVMLTLTSASLTGVIV
                     IMIPVVIVPMIIVGRRVRRLSRSAQDRIADTSSLVDETLNAIQTVQAFTLEELQSRRY
                     DAAVENSFKAAIRRSKVRAVLTAFGTALVFIGIALVLWMGARQVLTGQVSWGQLSQFF
                     LYALYVGTAAASLTEMWGEV"
     gene            1075..1587
                     /locus_tag="VNZ06_00835"
     CDS             1075..1587
                     /locus_tag="VNZ06_00835"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWW19321.1"
                     /translation="MALWKETPAPAPAPSGTPAAAGSTVTELHKGPSTAQDNDATRRL
                     ASAPKPAGGESVIGPELTIEGKIQGGGDVRIAGRFKGDVSVDGNFRIDSGARLEGQVK
                     ASVVVVGGELQGNIDAAKHVDVLSTGVIVGDVKAASITVAAGSRMRGHVEFGWDDKHS
                     SAIKGATRQV"
     gene            1584..2177
                     /locus_tag="VNZ06_00840"
     CDS             1584..2177
                     /locus_tag="VNZ06_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19322.1"
                     /translation="MSTARPGNLGATRVCPHCKATVLENSAVCPGCRHHLRFNTGSSP
                     QTVAEEGYCALDVDGTIRHKVTSEPCEYCVVLDVRNERGEQVVRQVVGVGVLQAGESR
                     RLHVAVDMLPVRAQVPAQQPAAKLPASVPKPPANPTPNSAKSFLKPPVPGQGLNAGTP
                     SKPAAAPAAPASAAAGSAPRTGSTGSAGSLFKSFRKP"
     gene            complement(2245..2994)
                     /locus_tag="VNZ06_00845"
     CDS             complement(2245..2994)
                     /locus_tag="VNZ06_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19323.1"
                     /translation="MKTMLAGAALALCAVACVTGGNSGSPGKVPPYVTAAVAEPTRPD
                     ADRKRDDDRKPDQVLTFSGIKPGDKVLELQPGGGYYTRLLCLVAGAKGQVTTLRVTPA
                     TPLNRPTPPDQPTPCKNISALTQKITELSLPSGQDAVWTSENYHDMHLPVFNADIKAF
                     NAAVYNALKPGGVFIIEDHAAQSGSGARDSGTLHRIDPDLVKQEVTSAGFEFVGASEV
                     LHRADDPMTGRVFELQGKTNKFLFKFRKPKK"
     gene            complement(3065..4456)
                     /locus_tag="VNZ06_00850"
     CDS             complement(3065..4456)
                     /locus_tag="VNZ06_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWW19324.1"
                     /translation="MLSVLAGCVSGPNFTAPPVPTTQAFTAAPLDTLSHSGMQVDTAA
                     IEPAQWWSLLNSPQLDATIRQGLKANHTLEAARSTLAQARALDVVSEAGRYPELTLDA
                     SSGRDKYGAAFLGPQKLPAFTFYSIGPAVSYSFDFAGGTRRKIEQQHAQTLYQQQELN
                     AAELTVSGNIARQALAAAAARAQIDNLLALLVDDRKDVQLVKDAFDAGSGTRVDVLTA
                     QSQLANDQTLLPPLRRDLSNATHAIAQLVGEAPADWSPPEFKLDEFALPAHLPVSLPS
                     ELAHRRPDILAAEAQLHEATAAIGVATANLYPQIGLTANASLQSNTLRTLFESSSSAW
                     GVVGSLTQPLYNHGELRAKQRAATEAMHAAAGNYQQVLLSAFTQVADALEGLDHDQEL
                     IGSEQTAVATAADNLELTRESYSAGNSGVLQVLEAQRQNQQARLGLVRAQSQRLQDMV
                     QLLLALGGQIPSD"
     gene            complement(4495..5610)
                     /locus_tag="VNZ06_00855"
     CDS             complement(4495..5610)
                     /locus_tag="VNZ06_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HWW19325.1"
                     /translation="MDAVADNLTEQRRLQQADDRRARLRWILMGGGVLVAVIFGLWYY
                     LTSGRYATTDDASVMAAQATISSNVPGRVVDVDVRDNQLVRKGDVLFRLDERPFQIAV
                     EEAQAKLAAARMQIIAGKATYRRELTSLTANKDTLSYQQHEFERQQRLLQSGISSRAQ
                     FEQVQHAVEMAQSNLASAEQQVSSVLAMLGGSADTPVDAHPVVLQAQAALDKAKLDES
                     YTVIVAPDDGVVAKVEQLQAGDYINAAAPLFTLVSARDVWIEANFKEDDLTYMRPGQT
                     AKVSIDAYPSHDCKAHVTSLSPGTGSQFSMLPAENATGNWVKVVQRVPVRLQLDDNCG
                     LGGAMPLHSGLSAEVTVDTEHHRTLLGGSTKSVASRG"
     gene            complement(5618..6097)
                     /locus_tag="VNZ06_00860"
     CDS             complement(5618..6097)
                     /locus_tag="VNZ06_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWW19326.1"
                     /translation="MKTNEATTSAPAPAPAERLRNFGFLMRDVSRLYAAYFQRHAREL
                     ELTLPQCKVLGHLSRNEGISQARLAELSDTDPMTLVRTLDRMQQDHWIERRADPADRR
                     AHRLYLREAAKPILNRMWKIADQCRQEAMASLSPEQREQLIELLERVHGTLSALEAK"
     gene            complement(6162..6686)
                     /gene="ppa"
                     /locus_tag="VNZ06_00865"
     CDS             complement(6162..6686)
                     /gene="ppa"
                     /locus_tag="VNZ06_00865"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF002317.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HWW19327.1"
                     /translation="MGLDLVTPGNQAPDIFNVIIEIPMAADPIKYEVDKETGAIFVDR
                     FMGTSMHYPCNYGYVPRTIADDGDPVDVLVITPFPLIPGVVVRCRPLGMLKMTDEKGG
                     DAKVLAVPDDRVLSIYSHWKRHQDIHPERLHQIQHFFEHYKDLEAGKWVRVEGWGSVE
                     QAKQEVAQGMSRYR"
BASE COUNT         1271 a         2254 c         2159 g         1325 t
ORIGIN      
        1 tgcacttcgc cccacatctc ggtcagactg gccgcagcgg tcccaacata cagcgcatac
       61 agaaaaaact ggctcagctg tccccagctc acctgcccgg tcagcacctg gcgcgcaccc
      121 atccatagca ccagcgcgat gccgatgaac accagcgcag tcccaaacgc ggtaagcacc
      181 gcgcgtacct tgctgcggcg gatggcggct ttgaagctgt tctccaccgc ggcgtcgtag
      241 cggcgacttt gcagctcctc gagtgtgaac gcctgcacag tctgtatggc gttcagcgtc
      301 tcatcgacca ggctgctggt atcggcgatg cgatcctgcg cgctgcgtga caggcgtcgc
      361 acccgccgac ccacgataat catcggcacg atcaccaccg gaatcatgat cacgatcacc
      421 ccggtgaggc tggcactggt cagcgtcagc atcaccagtc cgccgaccag actgagtatg
      481 gatcgcagcg tgattgatag gttgaccccg gcgagcgcct gcaccagggt ggtgtcagcg
      541 gtcaatcgcg acaggacctc acccgtgcgc gtggattcga agaacaacgg gtccatgcga
      601 atgacccggc gatagaccgc ctgtcgcagg tccgctacca cccgctcgcc tgcccaggtc
      661 accagataaa agcgcagcgc cgcaaatacg ccgaaggcca ccgaggcgcc caggaacccg
      721 aacaaatacc tattgaggat ggtggtattg gcggtcgtca gaccgtgatc aatcagctgc
      781 ctcagagccg ccggtgccgc cagcatcgcg ccggccgcca ccagcagcgc gagcagggcg
      841 ccaaacaaaa ttgcccggtg gggcaccagg aatggtaaaa gggccctcaa cgggcgtagt
      901 gacttggtac gcggtcggtc tgtaacatca ggcatgcaaa ctgatcctac cgcggcgcgg
      961 tctcggtcgt gtgaaccagt actggtctcc gttgaccgac gctggtgttc tggaccgatt
     1021 tggcggtacc gtgtggcgta gtcaaacctg cagatctaat tggaggatcc gctcatggcg
     1081 ctttggaaag aaaccccagc cccggctccg gctccgagcg gcactcccgc cgcggcgggc
     1141 agcacggtca ccgagctgca taaaggaccc agtaccgccc aggataacga tgcgacccgg
     1201 cgtctcgcca gcgcaccgaa gcccgccggt ggtgaatccg tgattggacc ggaactgacc
     1261 atcgaaggca agattcaggg cggcggcgat gtgcgcatcg ctggtcgctt caaaggcgat
     1321 gtcagcgtcg atggcaattt ccgcatcgac agcggcgcgc gactcgaagg acaggtaaaa
     1381 gccagcgtgg tcgtcgtcgg cggcgaactg caaggcaata tcgacgctgc caagcacgtg
     1441 gatgtcctgt ccacgggtgt catcgtcggc gatgtcaagg cagcctcgat cacggtggcg
     1501 gctggatcac gcatgcgcgg acacgtggaa ttcggctggg acgacaaaca cagctccgcc
     1561 attaagggtg caacccgcca ggtatgagca ctgcgcggcc tggcaatctg ggcgcgacac
     1621 gagtctgccc ccactgcaag gcaaccgtcc tggaaaactc agcggtgtgc ccgggatgcc
     1681 ggcatcacct gcgcttcaat acgggtagca gcccgcagac cgtcgccgaa gagggctact
     1741 gtgctctcga cgtcgacggc acgattaggc ataaggtaac cagcgagccg tgcgaatact
     1801 gcgtcgttct ggatgtgcgc aatgagcgcg gcgaacaggt ggtgcgccag gtcgtaggcg
     1861 tcggcgttct gcaggccggt gaatcgcgtc gtttgcacgt cgcggttgac atgcttccgg
     1921 tcagggcgca ggtgccggcg cagcaaccgg cggccaaatt gcccgccagt gtgccgaaac
     1981 cacccgccaa tccgaccccg aactcggcca agagctttct gaagcctccg gtacctggtc
     2041 aggggctgaa cgcgggtacg ccgagcaagc ccgctgcggc accggccgct ccggcttcgg
     2101 ctgccgcggg ttcggctccg cggaccggct cgaccggctc ggctggctcg ctgttcaaga
     2161 gttttcgcaa gccttgaaac ccgaaggcgg cgcggcgtca tttcagtgac gccgcgccgc
     2221 tgcgcgtgtg tgttctctta aggcctattt cttcggcttg cggaatttga acaggaactt
     2281 attggtcttt ccctgcagtt cgaacactcg accggtcatc gggtcatccg cccgatgcaa
     2341 cacctcgctg gcaccgacga attcgaagcc agcactggtg acttcctgct tcaccaggtc
     2401 cgggtcgatc cgatgcagcg tgcccgagtc gcgggcgccg gaaccgctct gcgcggcgtg
     2461 atcctcgatg atgaacacac cgccgggctt gagcgcgttg tacaccgcgg cgttgaatgc
     2521 tttgatgtca gcgttaaaaa ccggcagatg catgtcgtga tagttctcag aggtccacac
     2581 cgcatcctgg cctgacggca ggctcagctc ggtgatcttc tgcgtcagcg cgctgatgtt
     2641 cttgcagggc gtcggctgat cgggcggcgt cggccgattg agcggcgtcg ccggcgtcac
     2701 acgcaaggta gtgacctgtc cttttgcgcc tgcgaccaga cacagcagtc gcgtgtagta
     2761 accgccgccc ggctgcagct ccagcacttt gtcgccaggt ttgatgccgg aaaaggtcag
     2821 gacctgatcg ggcttgcggt catcgtcgcg cttgcgatcc gcatccggcc gggtcggttc
     2881 ggccactgcg gcggtaacgt atggcggtac cttgcccgga ctaccggagt tgccgcccgt
     2941 aacgcatgcg acggcacaca gcgccagtgc ggcgcctgcg agcatagttt tcatcggaat
     3001 atcccccttc gtagagcttg gaattggaaa ccaggtgcgc gagtttcgcc gagccatctt
     3061 accgtcagtc ggacggtatc tgtcctccca atgccaacag cagctgcacc atgtcctgca
     3121 ggcgctgcga ttgtgcgcgc accagcccta agcgcgcctg ctgattctgg cgctgcgctt
     3181 ccagcacctg cagcactcca ctgttgcccg cgctatagct ctcgcgcgtc agctcgaggt
     3241 tgtctgccgc ggtggcaacg gcggtctgct cgctgccgat cagctcctga tcgtgatcga
     3301 ggccttcgag cgcatcggca acctgcgtaa aggcgctcag cagcacctgc tgatagttgc
     3361 cggccgcggc gtgcatcgcc tcggtcgcgg cgcgctgttt ggcgcgcagt tccccgtggt
     3421 tgtacagcgg ctgggtcagg ctgccgacga ctccccaggc gctgctgctg gactcgaaca
     3481 gggtgcgcag agtgtttgac tgaaggctcg cattggccgt cagacctatc tgcggataga
     3541 ggttggcggt ggcgacgccg atggcagccg tggcctcgtg cagctgcgcc tcggcggcca
     3601 gaatatcggg ccggcggtgc gccagttccg agggcaagga caccggcaag tgcgccggta
     3661 gcgcgaactc atcgagtttg aattccggcg gtgaccagtc cgccggcgcc tcacccacca
     3721 gctgcgcgat ggcgtgcgtg gcgttgctca gatcccggcg cagcgggggc agcagcgtct
     3781 gatcattcgc gagctgactc tgcgcagtca gcacatccac acgcgtacca ctgccggcat
     3841 cgaacgcatc cttgaccagc tgcacgtcct tgcgatcatc caccagcagt gccaacagat
     3901 tgtcgatctg cgcgcgcgcg gccgcggcgg ccagcgcctg ccgtgcgatg ttgccgctga
     3961 ccgtcaattc cgcggcgttc agctcctgct gctggtagag cgtctgcgca tgctgctgtt
     4021 cgattttgcg acgcgtgccg ccagcaaagt cgaagctgta gctcacagcc gggccaatcg
     4081 aatagaacgt gaatgcgggc agtttctgcg gcccgaggaa tgcggcgccg tatttgtcgc
     4141 gacccgaact cgcatcgagc gtcagttccg gataccgccc ggcttcactc accacgtcca
     4201 gcgcccgagc ttgcgccagc gtcgagcggg ccgcctcgag cgtgtgattg gctttgagcc
     4261 cctgtcggat cgtcgcgtcg agctgcggcg agttcagcag cgaccaccat tgcgcaggct
     4321 cgatcgccgc ggtgtccacc tgcatcccac tgtgactcag ggtatcgagc ggcgccgcgg
     4381 taaaggcctg agtggtcggt accggcgggg ctgtgaagtt gggcccggac acgcagcctg
     4441 ccagaaccga cagcacgcac caagctagca gtcggcgtga tacgggcgcc gcattcatcc
     4501 gcggctggcc accgacttgg ttgatccacc caatagcgtc cggtgatgct cagtgtccac
     4561 agtcacctcg gcgctcaggc ctgagtgcag tggcatcgcg cccccaagtc cgcagttgtc
     4621 atccagctgc agtcgcactg gcacgcgctg cacgaccttc acccagttgc cggtggcatt
     4681 ctccgccggc agcatcgaga actgcgagcc ggtgcccggg ctgaggctcg tcacatgggc
     4741 tttgcaatcg tgtgacggat acgcatcgat gctcaccttg gcggtctgtc caggccgcat
     4801 gtaggtgaga tcgtcttcct tgaagttggc ttcgatccaa acatcgcgcg ccgacaccag
     4861 cgtgaacagc ggcgcggcgg cattgatgta gtcgccagcc tgcagctgtt caactttcgc
     4921 cacgacgccg tcatcgggcg cgacgatcac ggtataggac tcatccagct tggccttatc
     4981 gagcgcggcc tgcgcctgca gcaccaccgg gtgcgcatcc accggcgtat cggcactgcc
     5041 tcccagcatc gccaatacac tgctgacctg ctgctccgcc gaggccagat tggactgcgc
     5101 catctccacc gcgtgttgca cctgctcgaa ttgcgcgcgc gagctgatgc ccgactgcag
     5161 caggcgctgc tggcgctcga actcatgttg ctggtacgag agcgtgtcct tgttggcggt
     5221 gagactcgtg agctcacggc gataggtggc cttgccggcg atgatctgca tgcgcgccgc
     5281 cgccagtttg gcctgcgctt cctcgaccgc gatctggaac ggacgctcgt ccagacggaa
     5341 cagcacgtcg cccttgcgaa ccagctgatt gtcgcgcacg tccacgtcga ccacgcggcc
     5401 cggcacgttc gagctgatgg tggcctgggc cgccatcacc gacgcatcgt ccgtcgtcgc
     5461 ataacgaccc gatgtcaggt aataccaaag accgaaaatg accgccacca gcacaccgcc
     5521 gcccatgaga atccagcgca ggcgcgcacg tcgatcgtcg gcctgctgaa gacgtcgctg
     5581 ctctgtcagg ttgtcagcta cggcatccat agagatctca ctttgcttcc agagcagaca
     5641 gggttccgtg aacgcgctcg agcaactcga tgagctgctc tcgctgctcg ggactgaggg
     5701 acgccatcgc ttcctgacgg cactggtcag cgatcttcca catgcgattg agaatcggct
     5761 tggcggcctc gcgcagatac agccggtgcg cgcgccgatc cgccgggtcg gcgcggcgct
     5821 caatccagtg gtcctgctgc atgcgatcca gggtccgcac cagcgtcatc ggatcggtat
     5881 cgctcaactc ggccagccgc gcctggctga tgccctcgtt gcgcgagaga tgtccgagca
     5941 ccttacattg cggtagcgtc agctccaatt cgcgcgcgtg acgctggaaa tacgccgcat
     6001 acagacgcga cacgtcgcgc atgaggaatc caaagttccg cagccgctcg gccggagccg
     6061 gggccggggc gctggtcgtg gcttcatttg ttttcacagg agttataata acatttgcta
     6121 ttatatacgc aagtggccat tcacctccgg ccccccgggg actagcggta ccggctcatc
     6181 ccctgagcga cttcctgctt ggcctgctcg acgcttcccc agccctccac gcgcacccat
     6241 ttgccggctt ccagatcctt gtagtgctcg aagaagtgct ggatttgatg caggcgctcg
     6301 ggatggatgt cctggtggcg cttccagtgg ctatagatag agagcacgcg atcgtccgga
     6361 acggccagca ctttcgcgtc gccgcccttc tcgtcggtca tcttcagcat acccagcgga
     6421 cgacatcgca ccaccacgcc cgggatcagc gggaaaggcg tgatcaccaa tacatcgacc
     6481 ggatcgccgt catccgcgat cgttcgcggc acataaccat agttgcatgg atagtgcatc
     6541 gaggttccca tgaagcggtc gacgaagatc gcgccggtct ccttgtccac ttcgtatttg
     6601 attgggtcgg ccgccatcgg aatttcgatg atcacattga aaatatcagg cgcctgattg
     6661 cccggtgtca ccaggtcgag tcccattggt tgcctccgca gaactatgcg cgagcatcct
     6721 acaggcgaat atgtcgaccg gacatcaact attcgcgaca atgcgaagtg gcgttggagt
     6781 gtgaaagcag tcacgtcatc cgttactgct cctatgcgtt catagtcgta tcgaattcaa
     6841 ttgctacgga gaaggcatga tgcattttag aagcaccaaa gcgggcattg cggcgctagt
     6901 cgttgcggcc gtaatgctgg gaggctgtgt cgtgaccgca cgaccggcct atggagttgt
     6961 gacggttgca ccacccgccc ctcaggtgga ggtggtaggc gttgctcct
//
LOCUS       DATMRM010000031         4517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1567785, whole genome shotgun sequence.
ACCESSION   DATMRM010000031 DATMRM010000000
VERSION     DATMRM010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567785"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /gene="hisG"
                     /locus_tag="VNZ06_00870"
     CDS             complement(<1..450)
                     /gene="hisG"
                     /locus_tag="VNZ06_00870"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HWW19328.1"
                     /translation="MSKETRLRLAVQKSGRLTDPSLDLLARCGLKLSRGKDQLMGFGE
                     NMPLDVLFVRDDDIPDLVQEDVCDLGLVGLNVVQEKRLAFAGRAIAPLFETVQLLDFG
                     RCKLCIAVPDGFDYRGAQSLQGKRIATTYPHILERFLADRGISAEVVV"
     gene            complement(447..758)
                     /locus_tag="VNZ06_00875"
     CDS             complement(447..758)
                     /locus_tag="VNZ06_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HWW19329.1"
                     /translation="MKSHRQVSARQEALIERNLINAVLTLRSVEECRAFLRDLCTPAE
                     LQAMADRWAVVECLRRGLPYREVHRHTGVSVTTIGRVARYLAQGNGGYALAAQRVGAQ
                     P"
     gene            complement(842..1384)
                     /locus_tag="VNZ06_00880"
     CDS             complement(842..1384)
                     /locus_tag="VNZ06_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWW19330.1"
                     /translation="MLSALHLSDRLPRRAQGDHSLCSVSCRLHGHPAHACCRRHRAEH
                     RTQPELTGPQTSVMRAVYESKGITLKDLCKRVRLAHSTVSGIVDRLQARGLLERKPSD
                     EDRRYTIIAISPMVSQWMATHAPQLAAAPLQSALNQATSDEKEAILRGVATLLRLLEA
                     AQAGAGDHPTRDPQPSQPSV"
     gene            complement(1610..1951)
                     /locus_tag="VNZ06_00885"
     CDS             complement(1610..1951)
                     /locus_tag="VNZ06_00885"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWW19331.1"
                     /translation="MKRSHSTLYLCIAAAALTSAALPAMSNDTDRSPAVAVATDNLLL
                     FRVSSALQSDPYLYVRHIEVSLKDGAVLLTGFVTNDWELDKALRLATEAAKPHKVIDA
                     IEIEIKEGGRR"
     gene            complement(2058..2414)
                     /locus_tag="VNZ06_00890"
     CDS             complement(2058..2414)
                     /locus_tag="VNZ06_00890"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWW19332.1"
                     /translation="MQHARRSPAPTSLAGAAPAFAALGHATRLRIVMRLSQDGPLSIS
                     RLTDESQLTRQAITKHLRALEAAQLVSCERRGRQCIWQLRQSRLRDVRAYLEQISGHW
                     DAALERLRLLVERGKP"
     gene            complement(2424..2912)
                     /locus_tag="VNZ06_00895"
     CDS             complement(2424..2912)
                     /locus_tag="VNZ06_00895"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWW19333.1"
                     /translation="MSTDKIEKQILLRAPLERVWQAVSDSTQFGFWFGVSFDAPFRAG
                     ARLSGRITPTRVDPEVARLQAPHEGKKFEFWVERIEPLRSICFRWHPYAVSPDTDYSH
                     EPTTLIVFALRAVGSDTQLTITESGFDRIALERRAAALEANDGGWAHQIRNIEKYLAM
                     QP"
     regulatory      3078..3191
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3275..4285
                     /locus_tag="VNZ06_00900"
     CDS             3275..4285
                     /locus_tag="VNZ06_00900"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF006449.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HWW19334.1"
                     /translation="MSLAPQHVFSPDVLEDVLHLSARFSLHHGGSLEQPALGWRLAGR
                     ADAPIVVALGGISAHRCVFDRSQPREGWWHEVVGPGLALDTERVRVLGIDYLGGSAQS
                     SGPSAGGMFPSLSSYDQAQALAQLLDHLKIARLRAIVGASYGGMVALAFAERFAARVE
                     QLIVISGADRAHPMATAWRSVQRRIVRFGLSCGRSPEGLELARALAMATYRSADEFGM
                     RFDAPPRQSEGRFVFPVEEYLFARGRDYATRYRPESFLCLSESIDLHAVDAAHIHTPT
                     DLVAVREDQLVPITDMRALAMKLPRARLHELSSPHGHDAFLKEAAGLQPVFSSLYGVS
                     QR"
     gene            4282..>4517
                     /locus_tag="VNZ06_00905"
     CDS             4282..>4517
                     /locus_tag="VNZ06_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HWW19335.1"
                     /translation="MSDSADAVTRAVRAGLESDDATGAVVPPIHLSATYAFRGYGEKR
                     AYDYSRSGNPTRDLLAAAIADLEGGVSAVITSSG"
BASE COUNT          749 a         1450 c         1485 g          833 t
ORIGIN      
        1 aaccacgacc tccgcggata tgccgcggtc cgccagaaag cgctccagga tatggggata
       61 ggtcgttgca atgcgtttgc cctgaagcga ttgcgcgcct cgatagtcaa atccgtccgg
      121 taccgcgata cataatttac aacgcccgaa atcgagcagc tgcaccgtct cgaacagcgg
      181 cgcgatggcg cgccccgcga acgccaggcg cttttcctgg acgacattca atcccaccaa
      241 tcccaggtcg cacacgtcct cctgcaccag atccgggatg tcgtcgtcgc gcacgaacag
      301 cacgtcgagc ggcatgttct cgccgaagcc catcagctgg tccttgccgc gcgacagttt
      361 tagtccacag cgcgccagca gatcgagcga gggatcggtc agccggccgg acttctgcac
      421 cgccaggcgc aggcgtgtct ccttgctcat ggctgcgccc cgacgcgctg cgcggccagc
      481 gcgtagccgc cgttgccctg cgccaggtag cgcgcgaccc ggccgatggt cgtcacgctg
      541 acaccggtgt gccgatggac ctcccgatag ggcagtcccc gtcgcaggca ttcgaccacc
      601 gcccagcggt cggccatggc ctgcagttcc gccggcgtgc acagatcgcg caggaatgcg
      661 cggcattcct cgacgctgcg cagcgtcagc accgcgttga tgagattgcg ctcaatgagg
      721 gcttcctggc gggcgctcac ttgccggtgg gacttcatgc tgatccagtg tattaacgcg
      781 ctagtacact aacacaaggg cggatgagcg gcaagactga aagcggatcg gccggcaagc
      841 ctcacacgct cggctggctc ggctgcggat cgcgggtcgg gtgatcgccc gcgccggcct
      901 gagcggcctc gagcagccgc aacagcgtcg caacgccgcg caggatggct tccttttcgt
      961 ccgacgtggc ctggttcagg gcgctctgca gcggtgccgc cgccagctgt ggcgcgtgag
     1021 tggccatcca ttggctcacc atcggggata tcgcgatgat cgtgtagcgc cggtcctcat
     1081 cgctgggctt gcgctccagc aggccgcgcg cctgcagccg gtcgacgatg ccggagacgg
     1141 tgctgtgcgc cagacgtacg cgcttgcaca gatccttgag cgtgatgcct ttcgattcat
     1201 acaccgcccg catcacgctg gtctgcgggc cggtcagctc aggctgcgtt cgatgctctg
     1261 cgcgatgtcg ccggcagcaa gcgtgcgctg ggtggccatg cagcctgcag cttacagaac
     1321 aaagggaatg atcgccctgc gcacggcgcg gcagtcggtc agatagatgc agcgcgctca
     1381 gcagtaatcg gcactgcgat cagatggcgc tggccgaatg ccggcgcggt ttccgaacac
     1441 ggcgcagtat catgccggtg tagatcggat ggcgtaccag cgcgcaaccc ccgccgcccg
     1501 ggtggcggat gggctttcac gggcttgagc gcgtcgtgag cgacaggtcg tgtacgacgg
     1561 atatgagcgc caagcgcccc agggcgtcag caggccgtcg gaacgctcgt cagcggcgac
     1621 cgccttcctt gatctcgatc tcgatcgcat caatgacttt gtgcggcttg gcggcctcgg
     1681 tggcaagcct caaggccttg tccagttccc agtcgttggt gacgaagccg gtcagcagca
     1741 cggcgccatc cttgagggag acttcgatat gtcgtacgta caggtacgga tcggactgca
     1801 gggcgctgct gacccggaac agcagcaggt tgtccgtggc gacggcgacc gccggtgatc
     1861 tatccgtatc attcgacatc gccggcagcg cagcggacgt gagcgctgcg gccgcgatgc
     1921 aaaggtacag agtgctgtgt gatcgtttca cgataggttc ccctgctgta gcttctggta
     1981 cgtcattact gttaagtctg gtggcagtct ggcgcaaata ctgggcgtct aatgctcgat
     2041 ccagtgtggg aacattctca cggtttgccg cgctccacca gcaggcgcag tcgctcgagc
     2101 gccgcatccc agtgtccgga gatctgctcg aggtaagcgc gcacatcgcg caggcggctc
     2161 tgccgcagct gccagatgca ttggcgaccg cgacgctcac agctgaccag ctgcgccgct
     2221 tccagcgcgc gcagatgctt ggtgatggcc tgacgggtga gctgtgactc gtcagtgagc
     2281 cgcgagatcg acagcggccc gtcctggctc agtcgcatca cgatgcgcag ccgcgtcgcg
     2341 tgcccgagcg cggcaaaggc gggcgcggcg ccggcgaggg atgtcggcgc ggggctgcga
     2401 cgggcgtgct gcatcgttag cgctcagggc tgcatcgcca ggtacttctc gatgttgcga
     2461 atctggtgcg cccagccgcc atcattcgcc tcgagcgccg cggcgcgccg ttccaacgca
     2521 atgcggtcga acccggactc ggtgatcgtg agctgcgtat cgctgccgac ggcgcgcaac
     2581 gcgaacacga tcagcgtggt cggctcgtgc gagtagtcgg tgtcgggtga gaccgcatac
     2641 ggatgccagc gaaagcagat gctgcgcagc ggctcgatgc gctccaccca gaattcgaat
     2701 ttcttgccct catgaggcgc ctgcagcctg gccacttcgg ggtccacgcg agtcggcgtg
     2761 atgcggccgg acaggcgcgc accggccctg aagggagcat cgaagctcac gccgaaccag
     2821 aagccaaatt gcgtcgaatc gctgaccgcc tgccagacgc gctccaaggg cgcacgcagc
     2881 aggatctgtt tctcgatctt atctgtgctc ataggcaacc tcctggttgc atgagagcat
     2941 agttcaggct gccgcccgcg ggcaaccttg cgggtgccta ttggcggcgg gccagctgcc
     3001 aggccttatg tcgcggaatg cttggtcgcc gacggctcag cttgaccccc ggcgccggcc
     3061 ccttttagac tcctcttcac tcatcgagac cggctgaggg aaaggccctt agacgccggg
     3121 gcaaccgtcg gatatccgaa atggtgccaa ctcctgcggc ccggaaatca attccggccg
     3181 atagatgagg gccgattgat cagcagcagg ctgagcaggc gagcggtccg caggagtgcc
     3241 acggtgaaac gcaacaggca cagcggagcg cgaattgagt ttggccccac agcatgtttt
     3301 ctctcccgac gtcctcgaag acgtcctgca tttatcggcc cgattttcac tgcaccatgg
     3361 cggcagcctc gagcagccgg cgcttggctg gcgtctggcc ggacgtgcgg atgccccgat
     3421 cgtcgttgcc ttggggggca tttcggcgca tcgctgcgtg ttcgatcgat cacagccgcg
     3481 cgaaggctgg tggcatgaag tggtcggccc gggtctggcg ctcgataccg aacgggtgcg
     3541 cgtactgggc atcgactacc tgggcggcag cgctcagtcc tcaggtccga gtgccggtgg
     3601 catgtttccg agcctctcga gctacgatca ggctcaggcg ctcgcgcagc tgctggatca
     3661 tctgaagatc gcgcgtctgc gcgcgatcgt tggcgcttcc tatggcggca tggtcgcact
     3721 cgcgtttgcc gagcggttcg cagcgcgtgt cgaacagctg atcgtgatca gcggtgctga
     3781 ccgcgctcac ccgatggcca ccgcctggcg cagtgttcag cggcgcatcg tgcgtttcgg
     3841 gctcagctgc ggacgctcgc cggagggact ggagctggcg cgcgcgctgg cgatggccac
     3901 ctaccgatcc gccgatgagt tcggcatgcg cttcgatgcc ccaccgcgcc agagcgaggg
     3961 tcgtttcgtg ttcccggtgg aggagtacct gttcgcgcgt ggacgcgact acgccacgcg
     4021 ttatcgtccg gaatcgtttc tgtgcctgtc cgagtcgatc gatctgcacg cggtcgatgc
     4081 cgcgcacatc cacacaccca ccgatctggt ggcggtgcgc gaggatcagc tggtaccgat
     4141 cactgatatg cgcgcgctcg caatgaagct gccgcgcgcg cgactgcacg aattgtcctc
     4201 gccgcatggc cacgatgctt tcctgaagga ggccgccggc ctgcagcctg tgttttcgtc
     4261 cctatacgga gtgagccaac gttgagcgat tcagccgatg ccgtcacgcg cgccgtgcgt
     4321 gcgggtcttg aaagcgatga tgccaccggc gcggtcgtgc cgccgattca tctgagcgcc
     4381 acctatgcgt ttcgaggcta tggtgagaaa cgcgcttacg actactcgcg ttccggcaat
     4441 ccgacgcgcg atctgctggc cgcggccatc gccgatctgg agggcggcgt cagtgcggtg
     4501 atcacgagct ccggcat
//
LOCUS       DATMRM010000032         9499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2491388, whole genome shotgun sequence.
ACCESSION   DATMRM010000032 DATMRM010000000
VERSION     DATMRM010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2491388"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VNZ06_00910"
     CDS             complement(<1..313)
                     /locus_tag="VNZ06_00910"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="TrpG; with TrpE catalyzes the formation of
                     anthranilate and glutamate from chorismate and glutamine;
                     TrpG provides the glutamine amidotransferase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate/aminodeoxychorismate synthase
                     component II"
                     /protein_id="HWW19336.1"
                     /translation="MNARLLLIDNYDSFTYNLVQAFLVFGAAVEVYRNDAISLDQARA
                     LEPTHLCISPGPGTPAQAGISMAMIREFAGRIPVFGVCLGHQSIVEVFGGKVVRAKRL
                     MH"
     gene            complement(310..1695)
                     /locus_tag="VNZ06_00915"
     CDS             complement(310..1695)
                     /locus_tag="VNZ06_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HWW19337.1"
                     /translation="MKPPFDIAGDLDTPVSAFAKLAPLQPRFLLESVEGGERIGRYSF
                     IGFGDTLQVRLDEHGFQLGEEHRAVPRTRDELLNGLREALRRVPLPLPALGQVPLAGG
                     LVGYTSYDVVRFFERLPQHARALPGVPLLHYQAPRSLLVFDHVSRGIALLHAGDEAER
                     ASLRREVIQALQGSVPHIARSGSFAPPTPSLTRDQHMSGVRRAQEYIAAGDAYQLVLA
                     SRFVGQHALDPFQVYRALRLINPSPYMFFCRLGDLTVVGSSPEALVKLNRGHAELRPI
                     AGTKPRGEDPAEDLRLEQELRADIKENAEHVMLVDLARNDLGRVATAGSVHVDPYRII
                     ERYSHVMHIVSGVQGRLAPNRDAFDLFAACFPAGTLVGAPKVRAMEIIEELEPVRRGL
                     YGGTVGYFGAQGDMDHAITIRTLVFHGDEYSYQAGGGLVADSDPATEHDEVLAKSAAV
                     RRALELAEAGL"
     gene            complement(1910..2782)
                     /locus_tag="VNZ06_00920"
     CDS             complement(1910..2782)
                     /locus_tag="VNZ06_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HWW19338.1"
                     /translation="MTVRLLCAALLFMPALCAAYRVEDDDRATIELAGPAQRIVSLAP
                     GATAMLFAAGAGGRIVGTSAYSDEPAAAQKIERIGDAQSLDLERILALHPDVVVVWTG
                     GTSLTEIARLQRVGLRIYHHHVTRLEQMPDSLKRLGVLTGTQSEAAAAANELAGQIAS
                     LRSQYSMLSRASVFIQVWDKPLYTVGKDEIITDVIHMCGYRSTYDDLSDVSPAVTIES
                     VLARDPDVILALDSDPKSPQHWLLQWHAFGSMRAVRTGHLLAWSDPRLIRMGPSEVEA
                     AATLCKALHTVNAK"
     gene            2873..3778
                     /locus_tag="VNZ06_00925"
     CDS             2873..3778
                     /locus_tag="VNZ06_00925"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWW19339.1"
                     /translation="MTDLKLKDLRYLVAVADTRHFGRAAAKCFVSQPTLSAQLRKLED
                     YLGVQLIERRPRKVALTEAGENIAQRARSMLESGDAIVSLALTRRDPLAGALRLALLP
                     TIGPYLLPHVALRIRKALPRLELMLYEYRTNPLLERLQQGEIDVGILALPVQGDGLIT
                     RELYQEPFVAALPASHRLAGRARVRVEDLQGESVLLLEDGHCLRDQALAVCSRSGVQE
                     RHDFRATSIETLRQMVAAGVGVTLLPELASVGTYGATRGLTVLPFAKPVPTRTIGAVW
                     RKSTARAVAIDAVCDQIQRHSGLPS"
     gene            3775..5061
                     /locus_tag="VNZ06_00930"
     CDS             3775..5061
                     /locus_tag="VNZ06_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWW19340.1"
                     /translation="MSAPSVYDRMCWPEGQLEQALAGGEHRRELTAWLGESEYTLLST
                     LARQAATAKRRPDAPTIYLLPGILGSQLGVPRAAGEPPDVLWLDPDDVVNGRLTELHP
                     QHPTGVCSLGPVIYNYLALKLRLAAAGFHVVFHDYDWRHDIVASGRRLADRLKADSAT
                     QIALVGHSMGGLLARAALRHIDHECAPQRIRHVIGIGAPHGGAIAAVQALRAAYPVVC
                     RLAAIDQHHDAQNLTLEVFRYFLSLYQMLPTESPALNLFDVSSWPRTGAVPVPELLAS
                     ARGFASQLACADARFFSIVGIGQRTVTGIERQGEEFRYEITSSGDGTVAISRATLAGA
                     PLYCVRCEHSELPRSPTVADAVIDLLRAGRTRRLHSGVTQQPGHKVHLTDAMISRELG
                     GKLDWHKLSILQRRRYLDRLSEAPASYRFGQRRPAS"
     gene            5154..6872
                     /locus_tag="VNZ06_00935"
     CDS             5154..6872
                     /locus_tag="VNZ06_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWW19341.1"
                     /translation="MDRRGHFAFVRDLATRWDLIAAIVVFALLIFAIEASHGLLEPLS
                     KLNAEPLSLDPANLPFYAARTTLRMFAGLLFSLVFTLTYATWAAKSPRAEKLLVPILD
                     ILQSVPILTFLSVTVVFFMSLAPGRVLGAEFAAIFAIFTSQAWNMAFSFYQSLRTVPY
                     ELTEAADTFHLTPWMRFWQLEVPFALPALIWNVMMSMAGGWFSVVLSEFVTVGQTTFA
                     LPGIGSYIALATQQGNVKAIGYAIAAMILVILACDQLIFRPLVVWVDRFRVEQEPGTW
                     LPDSWALTMMRRSRIVAAVTTTFYATVRWTSWALPRPKRVISRLPRAVNRRLDMLWIG
                     LVVLLLVWGAWKIGVELIAQTTWSETLHVIGLASLTMLRVIVLIAVASLIWVPIGIRV
                     GLNPRAAEIVQPIAQFLASFPPQLLFPLAVYGIVTWKLNPDIWLSPLMILGTQWYILF
                     NVIAGATAIPNELKYAAENFQVRGWLWWRRVALPAVLPYFVTGAITASGGSWNAAVLA
                     ELASWKTSTVEAHGIGSYIAQATAAGDFQKCILGAVVMCVFVVVINRVLWRPLYYYAE
                     RKYRLT"
     gene            6884..8206
                     /locus_tag="VNZ06_00940"
     CDS             6884..8206
                     /locus_tag="VNZ06_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HWW19342.1"
                     /translation="MNAVPQINTAPLLEVEGVRKSFPKPDGAELLVLEGVNLRLVPGQ
                     IVGLLGRSGSGKSTLLRLIAGLAQPSAGTLQYMGKPIDGPADGIAMVFQSFALYPWLT
                     VFENVALGLEALRMEKAQIRKRSLQAIDLIGLDGFESAYPRELSGGMRQRVGFARALV
                     VHPNILLMDEPFSALDVLTAETLRTDFLDLWGEGRMPIKGVILVTHNIEEAVLMCDRI
                     LVFGSNPGRILTEIIVTLPQPRKRLDPVFREMVDRIYVEMTARPKGPSAVASKERFPG
                     QGIGTVLPRVSSNLLSGLMETVAAPPYNGTADLPKIASELQMEIDELFPVAETLQMMR
                     FAEVEGGDIKLLPEAIAFANSALDERKRIFARHLQNYVPLAAHVRRVLDERASHSARK
                     SRFIDELEDFMGEEAAEQTLRAIISLARYAEAFAYDDEHEMFSLENPT"
     gene            8251..>9499
                     /locus_tag="VNZ06_00945"
     CDS             8251..>9499
                     /locus_tag="VNZ06_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWW19343.1"
                     /translation="MARTTALLAKLSQPRLYRILDRERLFEVLDGARGRGATWISGPP
                     GAGKTSLAASFLQARKLSGIWYDVDAGDCDPASFFHYLALAAPKPRSARAQPLLALSP
                     ECLDLEGFTRRFLRVFYARLPAGAVVVFDNCHTVRTDAAFTKILKQVLDELPEGLGLI
                     LLSRADPPTTLARAEANGLLRRIGWESLRLTEEELRAIVLATHTASESTLAKLQQRSA
                     GWPVGLVLLLQRLQATDSIPSAAAEGAAIGSLNYFADEVFEQVEPATRDLLLRTSFLP
                     RVTAAHARELASSPNAAHVLEELFRQRLFTDRHAADQVSYQYHVLFREFLRSRASSVL
                     TAEELHALRASSARLLEQDRDFPAAFALRIENHDWDDAARLILKLARALVADGRWLTL
                     QTMIASLPAAQLERTPWLTLWLGA"
BASE COUNT         1663 a         3001 c         3025 g         1810 t
ORIGIN      
        1 catgcatcag ccgcttggcg cgtaccacct tgccaccgaa gacttcaacg atcgactgat
       61 ggccgagaca cacgccaaac acgggaatgc gccctgcaaa ttcgcggatc atcgccatcg
      121 agatccctgc ctgcgctggc gtaccggggc cgggggagat acacagatgg gtcggctcga
      181 gcgcgcgtgc ctgatcgagg ctgatcgcat cgttgcggta cacctcgacc gccgcgccga
      241 ataccaggaa cgcctgcacc aggttgtagg tgaaggaatc gtagttatcg atcagcagca
      301 gccgtgcgtt cacagtcctg cctccgcaag ctccagtgca cgccgcaccg cggcgctctt
      361 ggccagcacc tcatcatgct cggtggccgg atcgctatcg gccaccagac cgccgcccgc
      421 ctggtagctg tactcatcac cgtgaaatac cagtgtgcga atggtgatcg catggtccat
      481 gtcaccttga gcgccgaagt atccgacggt gccgccgtac aggccgcgcc gcaccggttc
      541 gagttcctcg atgatttcca tcgcgcgcac cttcggcgct ccgaccagcg tgcccgccgg
      601 aaagcaggcg gcgaacaggt cgaacgcatc ccgattggga gccagccgac cctgcacacc
      661 gctgacgatg tgcatcacgt ggctgtagcg ctcgatgatg cggtacggat cgacgtggac
      721 actgccagcc gtggcgaccc ggcctagatc gttgcgcgca aggtccacca gcatgacgtg
      781 ctcggcattc tccttgatgt cagcgcgcaa ctcctgttcc agccgaagat cctcggccgg
      841 atcctctcca cgcggtttgg tacccgcgat cggacgcagc tccgcgtggc cacgattgag
      901 tttcaccagc gcttcgggcg aggagccgac caccgtgaga tcgccgagcc ggcagaagaa
      961 catgtacggc gagggattga tgagccgtag cgcacgatag acctggaatg gatcgagcgc
     1021 atgctgaccg acgaagcgcg aggccagcac gagctgatag gcgtcaccgg cggcgatgta
     1081 ttcctgcgcg cgacgcacgc cgctcatgtg ctgatcgcgt gtcagcgacg gcgtcggcgg
     1141 ggcgaaggat ccgctacgcg cgatgtgtgg caccgagccc tgtagtgcct gaatgacttc
     1201 acgtcgcaac gaggcgcgct cggcctcatc gccagcgtgc agcagcgcga taccccggct
     1261 cacatgatcg aacaccagca gcgagcgcgg tgcctgatag tgcaacagcg gcacgccggg
     1321 cagcgctcgc gcgtgctgcg gcagccgctc gaagaaccgc accacatcgt aggacgtgta
     1381 gcccacgagc ccgccggcaa gcgggacctg acccagtgcc ggtagcggca gcggtacgcg
     1441 cctcaacgcc tcgcgcaagc cattcagcag ctcatcgcga gtgcggggaa cggcgcgatg
     1501 ctcttctccg agctgaaagc cgtgttcgtc gagacgcacc tgcagcgtat cgccaaatcc
     1561 tatgaaggag tagcgaccaa tccgctcacc gccttccaca ctctcgagca ggaagcgtgg
     1621 ctgtagcggc gccagcttgg caaaggccga gactggggta tccagatcgc cggcgatgtc
     1681 gaatggaggc ttcatatagt aaaaaaccca tcccggagcg tttccggaga tgggtctggt
     1741 atgcgtttgc cttgagtagt gtcgtcgatt acgggatgca gcaacgtcca gcgcccactc
     1801 ctattcgggg gcgccaccac catgaacggg tgaagacgtg gctgtacata ggtttcagag
     1861 tatggccagc ggcgtgacag ctcgtcaagc cacggcgtat taccgtcgtt tacttcgcat
     1921 ttaccgtatg tagcgccttg cacagtgtgg cagcggcctc cacctcgctc ggtcccattc
     1981 ggatcaggcg tgggtcggac catgccagca gatgcccggt tcgcaccgct ctcatcgagc
     2041 cgaaggcatg ccattgcagc agccagtgct ggggggactt gggatccgaa tccagcgcca
     2101 gaatgacgtc ggggtcgcgc gccagtaccg actcgatcgt gacggcgggc gagacgtcgc
     2161 tcaggtcgtc atacgtgctg cggtagccgc acatgtgaat cacatcggtg atgatctcgt
     2221 ccttgcccac ggtgtaaagc ggcttgtccc agacctgtat gaacacggaa gcgcggctga
     2281 gcatgctgta ctggctgcgc agcgatgcga tctgacctgc cagttcattg gccgcggcgg
     2341 ccgcctcgga ttgcgtaccc gtcagtacac ccagtcgctt caggctatcg ggcatctgct
     2401 cgagccgagt cacgtgatga tggtaaatgc gaagccccac gcgttgcagc cgtgcgatct
     2461 cagtcagcga cgtacccccg gtccacacca ccaccacgtc cgggtgcagc gccagaatgc
     2521 gctccagatc caggctctgg gcatcgccga tgcgctcgat cttctgcgcg gcggccggct
     2581 catccgagta ggcgctggta ccgacgatgc gtccgccggc acctgcggcg aacagcatcg
     2641 cagtggcccc cggtgcgagg ctcacgatcc gctgggccgg ccccgccaat tcgatcgtgg
     2701 cgcgatcgtc atcttccaca cggtaggccg cgcacagcgc aggcatgaac aacaaggcag
     2761 cacacagcag gcgcacagtc atctggttag cctacccgct tcgggctcga cagtcctgga
     2821 accggtcgat tgactttata ctggcgatag acagcattta tcgccatttc cgatgaccga
     2881 cctgaaactc aaggatctgc gctacctggt cgcggtagcg gacactcgcc acttcggccg
     2941 cgccgcggcc aagtgcttcg tcagccagcc gaccctttcg gcgcagctgc gcaagctgga
     3001 agactatctg ggcgtgcaac tgatcgagcg acggccgcgc aaagtcgccc tgaccgaggc
     3061 gggtgagaac atcgcacagc gcgctcgctc gatgctcgag agcggcgatg cgatcgtcag
     3121 tctggcattg acccgccgcg acccgctcgc cggagcgttg cgactggcgt tgctgccgac
     3181 gatcgggccg tatctgttgc cgcacgtcgc gctgagaatc cgcaaggcgc tgccccggct
     3241 ggaactgatg ctgtacgaat atcgaactaa tccgttattg gaacgcctgc agcagggcga
     3301 gattgacgtt ggtattctcg cgctcccggt gcagggggat ggtctgatca cccgcgagct
     3361 ctatcaggaa ccgttcgtgg cggcactccc ggccagtcat cgtctggccg ggcgcgcgcg
     3421 cgtgcgcgtc gaggacttgc agggcgaaag cgtgctgctg ctggaggacg gccactgttt
     3481 gcgcgatcag gcgctcgcgg tctgcagccg cagcggtgtg caggagaggc atgattttcg
     3541 ggcgaccagc atcgagacgc tcagacagat ggttgccgcg ggcgtgggtg tcacactgtt
     3601 gccggaactg gcgagtgtcg gcacgtatgg ggcgacccgc ggcctgaccg tgctgccatt
     3661 cgccaagccg gttccaaccc gaaccatcgg tgctgtgtgg cgcaagagca ccgccagggc
     3721 tgtcgccatc gatgcggtgt gcgatcagat acagcgtcac agcggattgc cttcgtgagc
     3781 gctccctccg tctacgatcg gatgtgctgg ccggaagggc agctcgagca ggcgttggca
     3841 ggcggcgagc accgccgcga actgaccgcc tggctgggcg agagtgagta cacgttgttg
     3901 agcacgttgg cgcggcaggc cgccaccgcc aaacgccggc cggatgcgcc cacgatctat
     3961 ctgttgcctg gcatcctggg atcgcaactc ggcgtgccgc gtgcggcggg cgaacctccg
     4021 gacgtgctgt ggctcgatcc agacgacgtg gtcaatggac gcctgaccga actgcacccg
     4081 cagcacccga cgggcgtgtg ctctctcggt ccggtgatct acaactacct ggccctcaaa
     4141 ctgaggctgg ccgcggccgg atttcacgtc gtctttcacg actacgactg gcgccacgac
     4201 attgtggctt ccggccggcg actggctgat cgcctgaagg cggattcggc gacacagatt
     4261 gcattggtcg gacacagcat gggcggtctg ctcgcacgtg cggcgctccg gcacatcgat
     4321 cacgagtgcg ctccgcagcg catccgccac gtcatcggca tcggtgcgcc gcatggcggc
     4381 gcgattgccg cggtacaggc actgcgggcc gcctacccgg tcgtgtgccg actggcggcg
     4441 atcgatcagc atcacgatgc ccagaacctg acgctcgagg tgttccgtta ctttctgagt
     4501 ctctatcaga tgctgcccac ggagagcccc gcgctgaatc tgttcgacgt gagcagctgg
     4561 cctcgcaccg gcgcggtccc ggtaccggag ctgctggcga gcgcgcgtgg attcgcatcg
     4621 cagctcgcct gcgcggacgc gcgattcttc tcgatcgtcg gtatcggaca gcgcaccgtg
     4681 actggcatcg agcgtcaggg cgaggagttc cgctacgaga tcacttcgtc cggtgatggc
     4741 accgtggcga tcagccgcgc gacactcgct ggggcaccgc tgtattgcgt gcggtgcgaa
     4801 cacagcgagt tgcccagaag tccgacggtc gctgacgcgg tgatcgacct gctgcgtgcc
     4861 ggacgcacgc gacgcctgca cagcggcgtg acgcagcagc cgggtcacaa ggtccatttg
     4921 acagatgcca tgatcagccg tgagctcggc ggcaaactgg actggcacaa actgtcgatt
     4981 ctgcagcgcc gccgctatct ggatcgcctc agtgaggccc ctgcatccta tcgattcggc
     5041 caacgtcgcc ctgcaagctg aagggcggtg ggggaaattg taagaaagct gtgcaacact
     5101 aggcggcccg agtcgtaggc tcgcgcttcc ttagaacaaa catctggccg ggcatggatc
     5161 gtagaggtca ttttgctttc gtacgcgatc tggcgacgcg atgggatttg atcgcggcga
     5221 tcgtcgtgtt tgcgctgctg atcttcgcga tcgaagccag ccacggactg ctcgagccgc
     5281 tctccaagct caatgccgaa ccgctgtcgc tcgatccggc caatttgcca ttttacgcgg
     5341 ctcggacgac gctgcgaatg ttcgcgggtc tgctcttctc gctggtgttc acactcacct
     5401 acgccacgtg ggctgccaag agtcctcgtg ccgagaaatt actggtcccg attctcgaca
     5461 tcctgcagtc ggttccgatt ctcactttcc tgtcggttac ggttgtgttc ttcatgtcgt
     5521 tggcccctgg ccgggtgctc ggcgccgagt tcgcagcgat cttcgcgatc ttcactagtc
     5581 aggcctggaa catggccttc agtttttatc agtcgctgcg cacggtgccc tacgaactca
     5641 ccgaagcggc cgacaccttc catctgaccc cctggatgcg cttctggcag ctcgaagttc
     5701 ctttcgcact gccggcgctg atttggaacg tcatgatgtc gatggccggt ggctggtttt
     5761 cggtggtgct ctcggagttc gtcacggtag ggcaaaccac gttcgcgttg ccggggattg
     5821 gttcctatat cgcactcgcc acccagcagg gcaacgtcaa ggcgattggc tacgcgattg
     5881 cggcgatgat cctggtgatc ctcgcgtgcg atcagctgat ctttcgtccg ctggtggtat
     5941 gggtcgatcg cttccgcgtc gagcaggagc ctggcacctg gctgcctgat tcctgggcgc
     6001 tgacgatgat gcgccgctcg cgcatcgtcg ccgcggtgac gaccacgttc tatgcaacgg
     6061 tacgttggac cagctgggcg ttaccacgtc cgaaacgcgt gatctcgcgc ctgccccggg
     6121 ccgtgaatcg gcgactggac atgctgtgga tcggtctcgt cgtgctgctg ctggtgtggg
     6181 gggcctggaa aattggcgtc gagctgatcg cccagaccac ctggagcgag acactccacg
     6241 tgatcggtct ggcctcgctg accatgctgc gcgtcattgt gctgattgcc gttgccagcc
     6301 tgatctgggt accgatcggc atacgcgttg gcctcaatcc gcgtgccgcg gaaatcgtgc
     6361 agccgattgc ccagttcctg gcatcctttc cgccgcagct gctgtttcca ctcgcggtct
     6421 atggcatcgt gacctggaag ctcaatcccg acatctggct cagcccgctg atgatcctgg
     6481 gcacgcagtg gtacatcctg ttcaacgtca tcgccggggc gacggcgatc cccaacgagc
     6541 tgaaatatgc cgccgagaat tttcaagttc gaggctggtt gtggtggcgc cgggtggcgc
     6601 tgccggcggt gctgccgtat tttgtgaccg gcgccattac cgcgtcgggg ggttcctgga
     6661 atgctgcggt cctggccgaa ctggccagct ggaagacctc cacggtcgag gcacacggca
     6721 tcggctcgta catcgcccag gcaaccgccg cgggcgactt ccaaaaatgt attctgggtg
     6781 ctgtcgtgat gtgtgtattc gtggtcgtca tcaaccgcgt gctgtggcgg ccgctgtatt
     6841 actacgccga gcgtaagtac cgcttgacct gaaggatgag tcaatgaacg ccgtaccaca
     6901 gatcaacacc gcgcctctgc tcgaggtcga aggcgtacgc aagtcgtttc cgaaaccgga
     6961 tggcgcggag ctgctggtgc tggaaggggt caacctgcga ttggttccgg gccagatcgt
     7021 cggtctgctc ggccgctccg gttcaggcaa gtcaacgctg ctgcgtctga tcgccggact
     7081 cgcgcagccc agtgctggca cgttgcaata catgggcaag ccgatcgatg gcccggccga
     7141 tggcatcgcc atggtgttcc aaagtttcgc actctatcct tggctgacgg tgtttgagaa
     7201 cgtcgcactc ggcctcgagg cgctgcgtat ggagaaggcc cagatccgca agcgttcctt
     7261 gcaggcgatt gacctgatcg gtctggatgg gttcgaatcg gcttatccgc gcgagctgtc
     7321 cggcggcatg cgtcaacgcg tgggcttcgc ccgtgcgctc gtggtgcacc cgaatatcct
     7381 gttgatggat gagccattct cggctctaga cgtgctgacc gcggaaacac tgcgcaccga
     7441 cttcctggat ctgtggggcg aaggccgcat gccgatcaag ggcgtgatcc tggtgacgca
     7501 caacatcgaa gaggcggtgc tgatgtgcga ccgcattctg gtgttcggca gcaacccagg
     7561 gcggatcctc accgagatca ttgtcaccct gccgcagccg cgcaaacgtc tggatccggt
     7621 gtttcgcgaa atggtggatc ggatctacgt cgagatgacc gcgcgtccca agggcccatc
     7681 ggccgttgcg agcaaggaac gcttcccggg ccagggtatc ggtaccgtac tgccgcgtgt
     7741 gtcatcgaac ctgttgtccg gcctgatgga aaccgtcgcc gcaccgccct acaacggcac
     7801 tgccgacctg cccaagatcg cgtccgaact gcagatggaa attgacgagc tgtttccggt
     7861 ggcggagacg ctgcagatga tgcgcttcgc ggaggttgaa ggcggcgaca tcaagctgct
     7921 accggaagcc atcgcgttcg cgaattcggc gctcgacgag cgcaagagga tttttgcgcg
     7981 ccacctgcaa aactatgtcc cgctggcggc gcacgttcgt cgggtactgg atgaacgcgc
     8041 atcgcactca gcccgcaaga gtcgtttcat cgacgagctc gaggacttca tgggagaaga
     8101 ggcggcggaa caaacgttgc gcgccatcat cagtctcgcc cggtacgccg aagcattcgc
     8161 gtacgacgac gagcacgaga tgttctcact ggagaatccg acctaacgca acgccagccg
     8221 atcggcgctt tgccgatcca ggagcgcacc gtggccagga cgaccgcgct gctcgcaaaa
     8281 ctcagccaac cccggctgta tcggatactg gatcgggaac ggctgttcga agtgctggat
     8341 ggagcgcgcg ggcgtggcgc gacctggatc tcaggaccgc ccggcgcggg caagacctcg
     8401 ctcgccgcca gctttctgca agcgcgcaaa ctcagcggga tctggtacga cgtggacgcc
     8461 ggggattgcg accccgcgtc cttctttcac tacctggccc tggccgctcc gaaaccgcgc
     8521 agcgcacgcg cgcaaccctt gctggccctg tcgccggaat gcctggatct ggaaggtttc
     8581 acgcgtcgct tcctgcgggt gttctatgcg cgcctgccgg ccggtgcggt ggtcgtgttc
     8641 gacaactgcc acacggttcg taccgacgct gccttcacga agatcctgaa acaggtcctg
     8701 gacgaactgc ccgagggact gggattgatc ctgctcagcc gcgctgaccc gccaacgacg
     8761 ctggcgcggg cagaggccaa cgggttgctg cggcgtatcg gctgggagtc gcttcgcctc
     8821 acggaggagg agttgcgcgc gatcgtgctc gccacacaca ccgccagtga gtccacgctg
     8881 gccaagttgc agcagcgctc ggcgggctgg ccggtgggtc tggtactgct gctgcagcgc
     8941 ttgcaagcga cggattcgat cccgtcggcg gctgccgagg gagcggcgat cggatcgctc
     9001 aactacttcg ctgatgaggt gttcgaacag gtcgaaccgg cgacacgaga tctgctgttg
     9061 cgcacttcat tcctcccgcg cgtcactgcc gcacacgccc gtgagctcgc ctccagtccg
     9121 aatgccgcgc acgtgttgga agagctattc cggcagcggc tgtttaccga tcggcacgcg
     9181 gcggatcagg tcagctacca gtaccacgtt ctgttccgtg agtttctgcg ctctcgtgcg
     9241 agcagcgttc tgaccgccga ggaactgcac gcactgcgag cgagcagcgc caggttgctg
     9301 gagcaggatc gtgacttccc cgccgcgttt gccctgcgca tcgagaatca cgattgggac
     9361 gacgcggctc gtttgatcct caaactggcg cgcgcactgg ttgcagacgg gcgctggctg
     9421 acgttgcaga cgatgatcgc atcgctgccg gccgcgcagc tggagcgcac accctggctg
     9481 acgctgtggc tgggtgccg
//
LOCUS       DATMRM010000033         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3731692, whole genome shotgun sequence.
ACCESSION   DATMRM010000033 DATMRM010000000
VERSION     DATMRM010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3731692"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VNZ06_00950"
     CDS             complement(<1..659)
                     /locus_tag="VNZ06_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19344.1"
                     /translation="MRLSILTAALQELTPRERRDADPDLAIEEWLEFARQIGCPNIEL
                     SAALHPTESDVPAAALLDPVANTLDLRRPFNAERAARVRRAMQSTSIGLSDLAYFDNM
                     LAADESVRRKKHAMMLRVFDAAVLLGTHAVAGFVGRNPLLGMDANLVMFAEVFVPLLK
                     EAKARALTFRVEQCPMPGWNITDSWHNNIAYAPGPWIALHRICERYGVGDQFRIHYDP
                     S"
     gene            complement(691..1923)
                     /locus_tag="VNZ06_00955"
     CDS             complement(691..1923)
                     /locus_tag="VNZ06_00955"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW19345.1"
                     /translation="MTRLTRFRLCSMMFLQFFIWGGWFVTLGSYLASSLHATGAQSAL
                     AYSTQSWGAIIAPFIVGLIADRYFNAERLLGAFHIAGGILLYALSGTRDLKAFYPCLF
                     AYMILYMPTLALVNTISFRQMEEPARHFSGVRVWGTIGWIVAGLGVSYLFAWDSPAGM
                     AQRSLGNTFLMCSLASFALGIYSFTLPRTPPLPNPEEQARFGRLLGLDALVLLRQRNF
                     VVFFVASILICIPLAFYYQNANQFLTEIHVANATGKQTIGQMSEVLFMFLIPVFLRTF
                     GMKTTLLLGMLAWVCRYLLFALGNAHELGFMLLVGIALHGACYDFFFVSGQIYSDSKA
                     GARHKGAAQGLITLATYGVGMLVGFWAAGLIDDRYSVGGTHDWRSIWLYPAAFAAVVS
                     VLFALTFRNERVAYANPH"
BASE COUNT          466 a          604 c          647 g          340 t
ORIGIN      
        1 tgcgaggggt catagtggat gcggaattga tcgccgacac cgtaccgctc acagatccgg
       61 tgcaatgcga tccacggacc cggggcatag gcaatgttgt tatgccacga atcggtaata
      121 ttccaccctg gcatgggaca ctgctcgacc cgaaacgtca gtgcgcgggc tttggcctcc
      181 ttgagcagcg gcacgaacac ctctgcaaac atcaccagat tggcgtccat tcccaacagg
      241 ggatttcggc ctacaaagcc ggcaacggca tgcgtgccga gcagtactgc ggcgtcgaac
      301 acgcgcagca tcattgcatg ctttttcctg cgtactgatt catctgcggc gagcatgttg
      361 tcgaagtagg ccaggtccga caggccgata ctcgtcgatt gcatggctct gcggacgcgt
      421 gccgcgcgtt ccgcattgaa gggccgccgc aaatccaggg tgttggccac gggatcaaga
      481 agtgccgcgg ccggcacatc gctttccgta gggtgcaggg ccgcggaaag ctcaatattc
      541 gggcaaccga tctgcctggc aaactccagc cactcctcga tggccaggtc cgggtcggcg
      601 tcgcgccgtt cacggggagt caattcctgt aacgctgcgg taagtatgct gaggcgcatg
      661 ggcctcggtc tgaatgcgcc cgccgaggga ctagtgcgga ttggcatacg cgaccctctc
      721 attgcgaaac gtcaacgcaa acagcacaga caccacggcc gcaaaggcgg ccggatacag
      781 ccagatcgac cgccagtcgt gtgtgccgcc gacggagtac cggtcgtcaa tgaggccggc
      841 agcccagaac ccgaccagca tgccaacacc gtaggtggcc agtgttatca ggccttgggc
      901 ggcgcctttg tgccgggcac cggccttgga gtcgctgtaa atctggccgg agacaaagaa
      961 gaagtcgtag caggcaccgt ggagcgcgat gccaacgagc agcatgaatc caagctcatg
     1021 cgcattgccc aaggcaaaca acagatagcg gcacacccag gcgagcatgc cgagcagcag
     1081 agtggttttc atgccgaagg tcctgaggaa taccgggatc aggaacatga acagcacttc
     1141 cgacatctga ccgatggtct gcttgcccgt ggcgttggca acatgaatct ccgttagaaa
     1201 ctgattggcg ttctggtagt agaacgccag tgggatacag atcaggatcg acgctacgaa
     1261 aaacacgaca aagttccttt gtcgcaacaa gacgagtgcg tcgaggccca ggagacgccc
     1321 gaagcgggcc tgctcctcgg ggtttggcag gggcggggtg cgcggcaggg tgaagctgta
     1381 gatgccgagt gcgaatgacg caagtgagca catcagaaag gtattgccaa gcgagcgttg
     1441 tgccatgccc gcgggcgaat cccaggcgaa caggtaactg acacccaagc cggcgacgat
     1501 ccaaccgatc gtgccccaca cacgaacgcc ggagaaatgt ctggcgggct cctccatctg
     1561 ccgaaacgag atggtgttaa ccagcgccag cgtcggcata tacaaaatca tgtacgcgaa
     1621 caggcagggg tagaaggcct ttaagtcccg tgtgccggat agcgcgtaca gcagtatgcc
     1681 gccggcgatg tgaaaggcgc cgagcaggcg ttcggcattg aagtaacggt ccgcgatcag
     1741 cccgacgatg aacggggcga tgatggcgcc ccaggattgc gtggagtagg ccagcgcgct
     1801 ctgcgctccg gtggcgtgca gactcgacgc caggtagctg cccagggtga cgaaccaacc
     1861 accccagatg aagaactgca gaaacatcat cgagcaaagg cgaaatcggg tgagtcgggt
     1921 cactgggatc ctacaagttc agccagaccg accccgcccg cgagctggcg accgcttgcg
     1981 cgatgaagcg caagccgcgc aggccctccg gtacgccggg ataatcccgc accggtggct
     2041 gacccagttc gacccgg
//
LOCUS       DATMRM010000034         6580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_943463, whole genome shotgun sequence.
ACCESSION   DATMRM010000034 DATMRM010000000
VERSION     DATMRM010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943463"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VNZ06_00960"
     CDS             <1..784
                     /locus_tag="VNZ06_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19346.1"
                     /translation="EQQRQAYFVKLVSPNILLSDQVRSFDDLMRLSDGPLGQMSQAEV
                     TALASYLETLGVYLRYVSKDLRRAVIFGDVPSLVSVPTAPVNDGNFTTIQDESAGAMK
                     VGSGTIAFGTVLISVVVAPATIGAAGETTLLVGAGVVAVGAGFLVTWGLLEIFGGDAP
                     PPSPAPKQPSSDNIDMPNEVDDQPTGEVEIPSAVAMGSPDNGIDVLSLVDELATGTLD
                     DVLSSFPIGWDSGSGTPLPGIGDLGGGDTGSGGSLGGDLGFG"
     gene            1544..2044
                     /locus_tag="VNZ06_00965"
     CDS             1544..2044
                     /locus_tag="VNZ06_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate pyruvate-lyase family protein"
                     /protein_id="HWW19347.1"
                     /translation="MTNTHNFSLFQKILLATDGTVTDLIALYTGEVIRVKKLEQEIRV
                     DQAPAELQCDGPTRLLRRKILLSGATRNYLCADSIFVFERFSKSIQDQLLNTDRPIGL
                     LWKEERLETYREIVDQHVEVNHDIARHFGLPDTEQLVSRTYLIHHHAKPVGSITEKWP
                     LSYFRH"
     gene            2136..2468
                     /locus_tag="VNZ06_00970"
     CDS             2136..2468
                     /locus_tag="VNZ06_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19348.1"
                     /translation="MRDLPASSATLVGATGALSLAMKPTVIDRSPTLAWVYGAWEPAR
                     GAGKTAANAGGKPTQATDLAGSGRYAERWVLLDSIYKESVPEPQAQYPALGWQVLLDD
                     GAGSSGAA"
     gene            2536..2712
                     /locus_tag="VNZ06_00975"
     CDS             2536..2712
                     /locus_tag="VNZ06_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWW19349.1"
                     /translation="MSWLSRESLYTANWMKYGFTPGMGAIGVLRKKLGIGLAQEMLLS
                     ARTYRGADLEPPRC"
     gene            3014..4018
                     /locus_tag="VNZ06_00980"
     CDS             3014..4018
                     /locus_tag="VNZ06_00980"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWW19350.1"
                     /translation="MRSEDDRDHQIRQCWRFVVKLLVTVAVAWYLLRKVPLANVRNAL
                     GGASPIWIALGFTVQIVIRLVNAWRIRLIAGAQGAPLSFVAILSVLFTSALYGLLLPG
                     SIGGGAATLVKYVGRGATAAAALASMVVNRLLETLTVMSFAAFFWGLAQLQAGAARSA
                     HLATILVIAAPLLLMGAYALLFGRIRLLARVEQWPVFGERRRLTRGLGALLQQCARAG
                     TLRATAAVGVCSLSMLKELLATLAAYCFARSLGIDLPFVAVAWMQSAVGLLVLLPITI
                     SGLGVREGTLVLATRTYGVEPATALSWGLVQFASLLGIVLIGGLIEARAWWLAPARST
                     "
     gene            4027..5091
                     /locus_tag="VNZ06_00985"
     CDS             4027..5091
                     /locus_tag="VNZ06_00985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW19351.1"
                     /translation="MTLCIVNFNGSHYLPPALEAAQPHAQFDEILVIDNASRDGSVEL
                     LRNRFPHCRVLQLESNRGPGGARNAGLAAAQCDWILFQDNDVRLTPGCVSTLVQALQD
                     SPDALLVAPRVLYADDRARVQYDSADCHWLGLMMPRNANRLAALTEVRAAATSSLVTA
                     CFLIDRTRCQTPLQFDERFGFNLEDHDFGVRACLEGHALLIEPHAQVLHSSGTPGLSF
                     RPGAVVPAARVYYLIRNRWYVLTKVFALRTLAVLAPVLLLYEFAQLLGALYKGWGRSW
                     GAALRDYFREWPRLLQERRDVQSSRRIGDARLLKGGPLPMTAAVNSALLERLLLGALQ
                     GIVSTYWLAARPLLKSAGNR"
     gene            5088..6281
                     /locus_tag="VNZ06_00990"
     CDS             5088..6281
                     /locus_tag="VNZ06_00990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWW19352.1"
                     /translation="MRIAMIAACPFPWPRGTPIRIHRLAEALGRRGHEVHVLTYHFGE
                     GALSADLTLHRIAGPSIYRHGAPGPTLTKLALLDPLLTYKLRRLMRALQFDVIHAHHY
                     EGMLCAWLARCGGRTPPLVFDVHTLLGSELPAYSAFGQGLLRSLGRALDRALPRRADH
                     LIAVSEEMRRSLGALTGLDESRITLISNGVETEHFGSGVASVERHTVVFAGNLAPYQG
                     LESLLQAFALVRQLEPSARLRLLTDGDFSPWRASARMLGLLDNIEVRQVGYAQLPVEL
                     CRAGVLVNPRSVCSGIPQKLLNYMASGRPVVSFAGSAAIMEHERTGLIVRDEEVAAFA
                     AAILRLMSDEALAMSIGARARAFVTEQYSWEQVAARVEQVYACLPGLRLLAPEIAADV
                     PPSLS"
     gene            6281..>6580
                     /locus_tag="VNZ06_00995"
     CDS             6281..>6580
                     /locus_tag="VNZ06_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWW19353.1"
                     /translation="MRLVMLGIDAAEHTLIDSLIARGRMRALARLRRTGTSGLLQSPA
                     DLYSGAVWPTFHTGQRPAWHGIYHNKLWQPHRMCCIVPDESSYRVRPFWERFGPGV"
BASE COUNT         1174 a         1846 c         2163 g         1397 t
ORIGIN      
        1 tgagcagcag cggcaagcgt atttcgtcaa gctggtatcg ccgaacattc ttctatccga
       61 tcaggtgcgc tcgttcgatg acttgatgcg cctctctgac ggaccgctag gtcagatgag
      121 ccaagcagaa gtcacagcac tggcatccta tctggaaacc ctcggtgtgt atttgagata
      181 tgtgagcaag gacctgaggc gcgcggtcat ctttggagac gtgccatccc tggtcagtgt
      241 cccgacggcg ccggtgaacg atggaaactt cacaaccata caggacgaat ctgcaggtgc
      301 catgaaagtc ggcagtggaa cgatagcctt cggtacggtg ttgatttccg ttgtggttgc
      361 tcccgccact atcggcgccg caggagagac gacattactt gtgggtgcgg gtgtggtagc
      421 cgtgggagct ggcttcctgg tcacctgggg gctgctggag atatttggtg gcgatgcgcc
      481 ccctccgtct cctgcaccta aacaaccgag ttcggacaac atcgacatgc cgaatgaagt
      541 ggatgaccaa cctacgggag aggtcgaaat ccctagtgcc gtggccatgg gaagccctga
      601 caatggcatc gatgtgctga gtctggtcga tgaattggcc actggaacac tggatgacgt
      661 gctctcaagt tttccaattg gatgggactc ggggtcgggc acgcctctgc ctggaattgg
      721 ggacctaggt ggtggggata caggaagtgg tgggagtctt ggaggcgact tgggatttgg
      781 ttgaacggtg gcagtacctt gaaaccgtcg aggtacgaca gcaatgttgc ctgctcgacg
      841 gcgccctggt acaggctcgc cagcgtggtg ttcgagtcgg ccgagctgcc gagacgccgg
      901 gcatagtcgg gataggaggc attgaactgc tggagattct cgaccaggcg gctctggtag
      961 aagtgctcgc ggcgagtcag cagcgtcgag acgaacgaga tgccgaaacg gcctccaagg
     1021 ttgcgcgcga gattgatcaa agccgatgcg ttgttggtct tatggggggc aacaccgcga
     1081 ctagcaggcg aatatcgact tatgttcata taggcatttg ccgatgttct agcagcgatg
     1141 tcctttggtg tgcccagaat tcccgtcatt accaatgtga cggcacagcc ttatccgacg
     1201 tacgacgtat ccgagtcggt taagtctctg ttgctgcgac agatagccag ttcggtgcaa
     1261 tggacaggag agcatccgtt tccttttgag ccaaggtatt agggaattca aaggcggagt
     1321 aattgcgcgt aaagcccttg gctggcgctc gatagcgggc tttgaagtgt ccccggtgcg
     1381 ttgatagcct tcgatcagcc gtgctagcta tgcgagtgaa caatcaccgc tgacacgcag
     1441 caggtaggct cgcagcggcc gttatcggcg cgacgtgcat ctcaaactat attggaaagg
     1501 gctcggacga gcacgtgctg tttcctgtcg gtatcactct caaatgacca acactcacaa
     1561 tttcagtctc ttccaaaaaa tcctgctggc cacagatggc acggtcaccg atctcatcgc
     1621 cctgtacacg ggcgaagtca ttcgagtgaa aaagcttgag caggagattc gtgtcgatca
     1681 agcgccggct gaactgcaat gcgatgggcc gacgcgattg ttaaggcgca agattctact
     1741 tagcggcgcg accagaaact atctgtgtgc cgactccatc tttgtgttcg agcgtttttc
     1801 caaatccatc caagatcaat tgctcaacac cgaccggccc attggattgc tgtggaagga
     1861 ggaacggttg gaaacctatc gggaaattgt cgatcaacat gtcgaagtta atcacgacat
     1921 tgcgcggcac tttggcttgc ccgacaccga gcaattggtc tcacgcactt atctcattca
     1981 tcaccatgcc aagcctgtag ggtcgattac ggagaagtgg ccgctgagtt attttcggca
     2041 ttgatcatcg ttgtgtatgc gaacctctaa cgtcgcggtt cggcgccgga gaaggcgcaa
     2101 cgaacacgac tggctgctct cgagcgatca agtagatgcg cgacttgccg gcatcgagcg
     2161 ccacgctggt cggggcgacc ggcgcgttgt cattggcgat gaagccgact gttatcgatc
     2221 gcagtccgac gctggcgtgg gtgtatggtg cgtgggagcc ggcgcgcggt gctggcaaga
     2281 cggcggcgaa tgcgggaggc aagccgacac aggccactga tttggcgggt tctgggcggt
     2341 acgcggagcg atgggttctg ttggactcta tatataagga gagtgtgccg gagccgcaag
     2401 cccagtatcc ggccctgggg tggcaggtgt tgctggacga cggcgcgggt tcgtcagggg
     2461 cggcatgatg agtcatttcc gccatgcagg gccatggcat cggcggtggg ttcatattcg
     2521 gatagtttgc agattgtgtc gtggctgagt cgcgaaagcc tctatacggc caactggatg
     2581 aagtatggct ttacgcccgg aatgggcgcg atcggtgtat tgcggaaaaa actagggatc
     2641 ggtctggcgc aggaaatgct attgagtgcg cggacctatc gcggtgccga tcttgagcca
     2701 cctcgttgct gaaaaccgcg cgcagttgcc cgcaatcgta cagcgagaag ttgcgatgca
     2761 cgatgaaact ttcatcagcc tgaagtcaaa gagcgtattc ggaggatgtt cggcaagtag
     2821 gtgacagcag acgcgtaata gcaattgaat taaacggtta gatcgctctg ttaaggtgcc
     2881 cgaccggtgg gttgccatcg gcagcgactc gcgcatcaat atgtcgcatc aaagtacgct
     2941 ttcgtccttc tgagcgctgg cgcagaatca gcgaaaggca aaggatgtca gcggggtagg
     3001 ccccggcagc aggatgcgca gcgaggacga cagggatcat cagatccggc agtgctggcg
     3061 ttttgtcgtc aaattgctgg taaccgtcgc ggtcgcgtgg tacctgctca gaaaagtccc
     3121 attggcgaac gttcgcaatg cactgggcgg tgcctcgccg atctggatcg cgctcggatt
     3181 tacggttcag atcgtgatcc gcctcgtcaa tgcctggcgc atccgcctca tcgcaggagc
     3241 gcaaggcgcg ccgttgtcct ttgtcgcgat cctttcggtg ctgttcacca gcgcgctgta
     3301 tgggctgctg cttcccggga gtatcggtgg cggcgccgcg acgctggtca agtacgtcgg
     3361 caggggagcc acagcagccg cggcgctcgc gagcatggtg gtcaataggc tgctcgagac
     3421 cctaacggtc atgtcgtttg cggcgttctt ctggggactg gcgcaacttc aggccggcgc
     3481 ggcgcggtcc gcgcacctgg cgaccattct tgtgatcgcc gcgcctttgc tgctgatggg
     3541 cgcgtatgcg ctgctgtttg gccgcattcg cttgctggcc cgtgtcgagc aatggcctgt
     3601 gttcggggag cgccggcgtc tgacccgggg gctgggcgca ctgctgcagc aatgcgctcg
     3661 ggcaggaacg ctgcgcgcca cggcggccgt gggcgtgtgc tcgctgtcca tgttgaaaga
     3721 gctgctggcc accctggcag cctactgttt cgctcgctcc ctcgggatcg atttgccgtt
     3781 cgtcgcggtg gcctggatgc aaagcgcggt cgggttgctg gtcctgttgc cgattacaat
     3841 ctccggactg ggtgtgcgcg agggcacgct ggtgcttgca acgcgcacct acggtgtgga
     3901 gccggccacg gccttaagct gggggctggt ccagtttgca tcccttctcg gcattgtgct
     3961 gatcggtggc ctcatcgaag cgcgggcctg gtggctcgcg cccgcgcgat cgacatgaag
     4021 ccatcagtga ctctgtgcat cgtgaacttc aatggcagtc actacctgcc gccagcactc
     4081 gaggcggcgc agccgcatgc acagttcgac gagattctgg tgattgacaa cgcgtcgcgt
     4141 gacggcagtg tcgagctgtt gcgcaatcgc tttccacact gccgcgtgct gcagctcgag
     4201 agcaatcggg gaccgggcgg ggcgcgcaat gccggtctcg ccgcggcaca atgcgactgg
     4261 atcctgttcc aggacaatga tgtgcggctg acaccaggat gcgtctcgac gctggtgcag
     4321 gcgttgcagg actcgcccga tgcgttgttg gtcgctccgc gcgtgctgta cgcggatgat
     4381 cgagcgcgcg tgcagtatga cagcgccgac tgccactggc tcgggctgat gatgccgagg
     4441 aatgcaaacc gcctggcggc cctgaccgag gtgcgagcgg cggcgaccag ctcgctggtg
     4501 acggcctgct ttctgatcga tcgtacgcgc tgccaaacac cgctgcagtt cgatgagcgc
     4561 ttcggcttca atctcgagga tcacgacttc ggcgtgcgtg cgtgtcttga ggggcatgcc
     4621 ctgctgatcg agccgcacgc gcaggttctg cattcaagcg gtacgcctgg attgtcattt
     4681 cgtccagggg cggtggtgcc tgccgcacgc gtctactatt tgatccgcaa ccgctggtat
     4741 gtgctgacca aggtcttcgc gctgcgcacg ctggcggtgc tggcgccggt tctgctgctg
     4801 tatgagttcg cccagctcct cggtgccttg tacaagggat ggggccgcag ttggggcgcg
     4861 gcgcttcgtg actactttcg cgaatggccg cgcctgctgc aggagcggcg cgacgtgcag
     4921 agcagccgcc gcatcggtga tgcgcgattg ctcaagggcg gacccttgcc tatgaccgct
     4981 gcggtcaatt ccgctctcct ggaacgcctg ctgctcggtg ctctgcaggg gatcgtcagc
     5041 acctactggc tggcagcgcg gccgctgctc aagagcgctg gtaaccgatg agaatcgcga
     5101 tgatcgccgc ctgtccgttt ccgtggccgc gcggcacgcc gatccggatc catcgtctgg
     5161 cggaagctct tggtcgccgc ggccatgagg tgcacgtgct gacttatcac ttcggtgagg
     5221 gcgcgctgtc agctgatctg accttgcatc ggatcgccgg tccgtcaatc tacaggcacg
     5281 gcgcgccggg accgacgctc accaagctcg cgctgctcga cccgctgctg acctacaagc
     5341 tgcgacgcct gatgcgcgcg ctgcagttcg atgtgatcca cgcgcatcac tacgaaggga
     5401 tgctgtgcgc ctggttggct cgctgcggcg gccgtacccc gccactggtg ttcgacgtgc
     5461 atacgctgct cggcagtgaa ttgccggctt atagcgcgtt cgggcaaggg ctgctgcgaa
     5521 gtcttgggcg cgcgctcgac cgcgctctgc cgcgtcgggc cgatcacctg atcgcggtgt
     5581 ccgaggagat gcgccgctcg ctcggtgctc tgacgggcct ggatgaatcg cgaataacgc
     5641 tgatcagcaa tggtgtcgaa accgagcact ttggatccgg cgtggcaagt gtggagcgcc
     5701 atacggtggt gttcgctggc aatctggctc cctatcaggg actcgagtcg ctgctgcagg
     5761 cctttgcgtt ggtgcgtcag ctcgagccct cggcacgtct gcgcttgctg acggatgggg
     5821 acttcagccc ctggcgcgcg agcgcccgca tgcttgggct gctcgacaac atcgaggtca
     5881 ggcaggtcgg atacgcgcag cttccggtgg aactttgccg ggccggggtc ctggtcaatc
     5941 cacgctccgt ctgcagcggc ataccccaga agctgctgaa ctacatggcg agtgggcgcc
     6001 cggtcgtatc attcgcgggc tccgctgcga tcatggaaca cgagcgcacc gggctcatcg
     6061 tcagggacga ggaggtggcc gcattcgcgg cggcgatcct gcggttgatg tccgatgagg
     6121 cgctggcgat gagcatcggc gcgcgcgcac gggcgttcgt aaccgagcaa tacagttggg
     6181 agcaggtcgc agcccgcgtc gaacaggtgt atgcctgtct tcccggactg cggctgctgg
     6241 cgccggagat tgcggcagac gtgccgccga gccttagttg atgcgactgg tgatgctggg
     6301 catcgatgcc gccgagcata cgctcatcga ctcgctgatc gctcgcgggc gaatgcgggc
     6361 actggcgcgc ctgcgtcgga caggtacctc cggtctgctg caatcgcccg cggatctgta
     6421 ttcaggtgcc gtgtggccga cctttcatac gggacagcgt ccggcctggc acgggatcta
     6481 tcacaataag ctctggcagc cccatcgaat gtgctgcatc gtgcccgatg agtccagtta
     6541 tcgcgtgcgt ccgttctggg aacgattcgg gcccggggtt
//
LOCUS       DATMRM010000035         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2492465, whole genome shotgun sequence.
ACCESSION   DATMRM010000035 DATMRM010000000
VERSION     DATMRM010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2492465"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..483)
                     /locus_tag="VNZ06_01000"
     CDS             complement(82..483)
                     /locus_tag="VNZ06_01000"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HWW19354.1"
                     /translation="MRTYPHNSPQAAARIVALAMLADGNPSKSEFDAIERMDVYRQFR
                     LAPGELDSIIETCCADLLALNRLSWDQSVRFDQRMLAQILSEVDDPALRRQVVRVCSE
                     VVEADHYVSENEWTLLSAMANQWNTHPKSCT"
     gene            complement(480..842)
                     /locus_tag="VNZ06_01005"
     CDS             complement(480..842)
                     /locus_tag="VNZ06_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19355.1"
                     /translation="MQLAEVNGPRQEVYKRCQVELRTDAAAPVVITSLARDWRSALDN
                     ALSRAARSVLWLWRRQRKTAMLQGRPLEDHRLSEHAVAKAHAMEAEQASANGHERTKS
                     ATTASEQVKHQTCLLATL"
     gene            1056..1946
                     /locus_tag="VNZ06_01010"
     CDS             1056..1946
                     /locus_tag="VNZ06_01010"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWW19356.1"
                     /translation="MDATFSYRHLYYFWVVAKEGGMSRAALKLDVAVQTVSTQVRELE
                     RSLGHVLLKPAGRGLVLTDAGLAAMRQADQIFQLGEQLPGAVRDAVSSPSIRLNIGVL
                     DILPKLVVWRLLKPVTREPKLRLQCQVDEFQDLLGDLALHRFDLVLADRQAPASPNLK
                     LYSHLVDESDLAWYATGTLQAKARRDFPKSLAHVPVLLPTHHAAVRGALDRWFERIGV
                     RPHVVGEFEDSALLETFGAAGMGVFPAADMTHDDLVAQYRVHRVGRCEGVREEFFAVG
                     TEKKIAHPLVQRLVRKSAKN"
     gene            complement(2040..>2274)
                     /locus_tag="VNZ06_01015"
     CDS             complement(2040..>2274)
                     /locus_tag="VNZ06_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19357.1"
                     /translation="PVNSANWQVSSVALPSPAEAGSSVTFSFRVSAPTAPGTYDFHWQ
                     LAHGSKFFGATSTNVAIKVVAPPSVSRSSDPKL"
BASE COUNT          450 a          678 c          731 g          415 t
ORIGIN      
        1 atacgccgag atcgaacgcg agcaggagtg cgacaaccgc gacaaatccc agccacatcc
       61 aggcagcgtg gccgagaagt gtcacgtgca ggatttggga tgcgtattcc attgattggc
      121 catcgcgctg agcaaggtcc actcgttttc ggacacatag tggtctgcct cgacaacctc
      181 gctgcagacg cgcacgacct ggcggcgaag cgccggatcg tccacctcgg agaggatctg
      241 cgcgagcatg cgttggtcaa agcggaccga ttgatcccag ctcaggcggt taagggcgag
      301 cagatccgcg caacaggtct caatgatcga gtccaactcc ccgggcgcca gtcggaactg
      361 gcggtagacg tccatccgct cgatcgcatc gaattcggat ttggacggat ttccatcggc
      421 cagcatcgcc agcgcgacaa tgcgcgcagc ggcctggggg ctgttgtgcg gataagttct
      481 cataacgtcg ccaacaggca ggtctggtgt ttcacctgtt cggaggccgt ggttgcgctc
      541 ttcgtccttt catggccgtt cgcggacgcc tgctccgctt ccatggcatg cgccttggct
      601 accgcgtgct cgcttaggcg gtgatcctct agaggacggc cctgcagcat cgcggtcttg
      661 cgctggcggc gccacagcca cagcacggag cgcgcggcgc gcgacagggc gttgtccagg
      721 gcgctgcgcc aatcgcgcgc cagcgacgtg atcaccaccg gggccgcggc gtcggtgcgc
      781 aactcgacct ggcagcgctt gtagacctcc tgtcgcgggc cattgacttc ggcaagctgc
      841 acacgtgccc gcggaaccag ccaacccagc cgccgcatta cgaagcgcgt acgccggtca
      901 gctcgtctgc gcaattggga actgtgtgaa atgagggatt cgaccagaac gcgcatggca
      961 caccccgcgg attcggaagg aagctcaacg ataggtcgcg cgcggttgcg agaaaagaag
     1021 ataatcgtga aagaacattc agggaaaaac tgataatgga cgcgacgttc agctaccgcc
     1081 acctctacta cttctgggtc gtcgccaaag aggggggcat gtcacgagca gcgctgaaac
     1141 tcgacgtggc cgtacagacg gtgagcacgc aggtccggga actcgagcgc tcgctcgggc
     1201 atgtgctgct gaagccggcg ggtcgcggcc tggtgctcac cgacgcgggt ctggcggcga
     1261 tgcgacaggc agaccagata tttcagctgg gcgagcaact gccgggtgct gtacgcgatg
     1321 ctgtcagctc gccatcgata cgcctgaaca tcggcgtgtt ggacatcctc cccaagttgg
     1381 tggtgtggcg cctcctgaag cctgtgacgc gggagccgaa attgcgcctg cagtgccagg
     1441 ttgacgaatt ccaggacctt ctcggggatc tcgcactgca ccgcttcgac ctggtgctcg
     1501 cggatcgcca ggcgccggcc agcccgaacc tgaagctata cagccacctc gtcgatgaat
     1561 cggatctggc gtggtatgcg acagggactc ttcaggcgaa agcgcgccga gactttccga
     1621 agtctctggc acacgtgccg gttctcctgc ccacgcatca cgccgctgtc cggggagccc
     1681 tggaccgatg gtttgaacgg atcggcgtgc ggccgcacgt ggtcggcgag ttcgaggaca
     1741 gtgccttgct cgagactttc ggtgccgccg gaatgggcgt gtttccagcg gcagacatga
     1801 cccacgatga tctggtggcg cagtacagag ttcaccgtgt cgggaggtgc gaaggcgtga
     1861 gggaggagtt ctttgccgtc ggaacagaaa agaaaatcgc gcacccgctc gtgcagcgtc
     1921 tggtgcgaaa aagtgcgaag aattgagggg ctgggcgatg cagtgtaagc atcgagctga
     1981 cgctgtttcg cgctcaagaa atccagtacg cgcctgacta atttagctcg tccggtcgtt
     2041 caaagcttcg gatcgctcga acgcgacaca ctcggcggcg cgacaacctt gatcgcaaca
     2101 ttggtcgaag tggctccgaa aaacttcgag ccatgcgcca actgccaatg gaagtcgtag
     2161 gttcctggcg ctgtcggagc acttactctg aaactgaacg ttacgcttga accggcctcc
     2221 gccggcgacg gcaaggcgac agaggacacc tgccagttgg cgctgttgac cgga
//
LOCUS       DATMRM010000036         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4045773, whole genome shotgun sequence.
ACCESSION   DATMRM010000036 DATMRM010000000
VERSION     DATMRM010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4045773"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..917)
                     /locus_tag="VNZ06_01020"
     CDS             complement(114..917)
                     /locus_tag="VNZ06_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017693479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWW19358.1"
                     /translation="MSEPLVLSSDSESVRTLRLNRPASLNSFNAALHEQLLKALDEAA
                     EEPGVRCVVLTGAGRAFCAGQDLTEASVAPATSPGAKRPDLGELTDRLYKPLVQRLRS
                     MPVPTVAAVNGVAAGAGTNIALNCDLVIAAHSASFIQGFGKIGLIPDSGGTWLLPRLV
                     GRARALGLAMLGDKLPAVDAERIGLIWRCVADDELPAQTLALAQKLASLPIKALVSTR
                     RCIDAAQLVDVDEALDSETSTQRILGQGHDYAEGVAAFKDRRTPHFTDR"
     gene            complement(2209..>3109)
                     /locus_tag="VNZ06_01025"
     CDS             complement(2209..>3109)
                     /locus_tag="VNZ06_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWW19359.1"
                     /translation="YAVGWSWAAAGAFGVLISATDPVSVIATFKEAGVHGRLRLLVEA
                     ESLLNDGTAAVAFVVVLGLATGSDSGWLATTMHFLLVFGGGVVAGLIVGFALKLFAAR
                     TDDQVVEMTLTALAAYGSFFLAKHFHVSGVLASLTAGIMTGSYRGPDSPWAAGRARLE
                     SLWEYIAFVVNSLIFLLIGAQVARQHFSGMWWAVRGAIALVTLGRALAIYPLCALFAR
                     TELKVSMRYQHVLFWGGLRGALALALALGLPPELPQHDDVVTVSFAVVVFSVFVQGLT
                     MQPLLRRLGELKGERMASSDPRT"
BASE COUNT          610 a          983 c          937 g          579 t
ORIGIN      
        1 gcttcgaagc gttccgagag tgccggatcg gccgcgacct gttctgtgcc atcgctacgc
       61 tccttgcctg ccgtgtccat tgcctgggta tacatacgcg cggtatcggt gctctagcgg
      121 tcggtgaaat gcggcgtgcg ccggtccttg aatgccgcga cgccttcggc gtagtcgtgt
      181 ccctgcccca ggatgcgctg cgtgctggtc tcgctatcca gcgcctcatc cacatcgacc
      241 agctgcgccg catcgatgca gcgtcgagtg gacaccagcg ccttgatcgg cagcgaggcc
      301 agtttctgcg ccagcgctaa agtctgcgcc ggcagttcat cgtcagcgac gcagcgccag
      361 atcagtccga tgcgttccgc atcgaccgcg ggcagcttgt cgcccagcat cgccagcccc
      421 aacgcgcgcg cgcgtcccac cagtcgcggc agcaaccacg taccgccgct gtcgggaatc
      481 aacccgattt tgccgaaacc ctgaatgaag ctggccgagt gcgcggcgat caccagatcg
      541 cagttgagcg cgatattggt gcccgcaccg gccgccacac cgttgaccgc cgccaccgtc
      601 ggcaccggca tcgagcgcag ccgctgcacc agcggcttgt agaggcgatc ggtcagctcg
      661 ccgagatccg gccgcttcgc gcccgggctt gtggccggag ccacgctcgc ctccgtcagg
      721 tcctgccctg cgcagaacgc gcgtcccgct ccggtcagca ccacgcagcg cacgccgggc
      781 tcttcggccg cctcatcgag cgcctttagc agctgctcat gcagcgccgc gttaaaactg
      841 ttgagcgacg ccggacgatt gagcctcagc gtgcgcaccg attccgaatc agacgacaac
      901 accaggggtt ctgacattgt gtagtcccgt acctattgat tgaccgaccg gtcggtcgga
      961 ttatagagcc gctgacaagg acccgcaact cttgcacgac ttgcgatcat gctggcatag
     1021 tcgaaccgtg agattttccg aactattgct ggtcgtccct ctgcgccgcg agcacgtcac
     1081 cgctgtgctt gcgaaatcgg ttccctcccg tatcccgaac ctcccgatcg cgattagcta
     1141 gggtcgtgcg ctgcggcgtg agccggtgcg catcgacccc cgagatccgg atcgatcaac
     1201 ttagttgtcg aacctcgtct accgtcggta cagaccgcgt ctggggtctt gcgttccgtt
     1261 cgcgtacccg aggtcggagg aatttggcgt ggaatttcga gtggctgggg acgatgcgga
     1321 gcgcattgcg gagcttcatg cgcagagttg gcgccggaca taccgcggca tgatgcgcga
     1381 tgagtttctg gatggacccg tggtcgcgaa tcggctcgac gcatggcaga agcgcttgag
     1441 cgaaaacttg gtagaccggt gggtctatct cgccaccgat ggttctcaac tcgttggatt
     1501 catttgtgtc ttcggaaatg aagatccgat ttggggttcg tacatcgaca atctccatgt
     1561 gtccgcggac taccaccggc gaggaatcgg ggccgtctta atgggtcatg cggcagcatg
     1621 gcttggcgct aggtatcctg gttcgggtgt gtatctatgg gtaatgatgg caaacgagtc
     1681 ggccaggcga ttctacgagc ggcttggcgc gactgacgcc gggacaatca ttaagtgcga
     1741 tcccggcggc ggacatgcac ctaactgccg ctacgtgtgg cgggaccccg cagctcttcg
     1801 acagcatgcg actgagtgga tgcccgggac gactccccag atagactttc gacgctcgtc
     1861 ggcctgatag aggtactcaa ttgtggccaa gggcccggtg atagagaccc gtctggcaag
     1921 tgcggccgat gccgacggca tcgcagccgc gcatcttgat tcgattcgca cgatcggtgc
     1981 ccgattttag tcgccggaag tcgtggacga ttgggctgcc cggattagca gcgatcgcta
     2041 cgtaaaagcc atgggaaacg gagagacctt ctatatcgcg gtcactaaca acaaccggtg
     2101 taaccgcggc cattgcggat gcgaggctga aagattggca agcttgggag cagcggtaag
     2161 ggcaggtgct cgctgctgca gagggtctat gatgtcgccc ctttggggtt atgtgcgcgg
     2221 gtcgctcgat gccatgcgct cgcctttcag ttcgcccaat ctgcgcagta gcggctgcat
     2281 cgtcagaccc tggacaaaca cagagaacac cacgaccgca aaggagacgg tgacaacatc
     2341 gtcgtgctga ggcagctctg gcggtagtcc gagcgcgagc gccagagcta gcgccccgcg
     2401 caggcctccc cagaagagca catgctggta tcgcatcgat actttgagtt cagtgcgggc
     2461 aaacaacgcg cacagcgggt agatcgccag cgctcgtccc agcgtcacca gggcaattgc
     2521 accgcgcacg gcccaccaca taccgctgaa atgctgccgc gccacctgcg cgccgatcag
     2581 caaaaaaatc agggaattca cgacgaacgc aatgtactcc caaagcgatt ccaatcgcgc
     2641 gcgccccgca gcccaaggtg aatcagggcc acggtagctt ccagtcatga ttccggcagt
     2701 gagtgatgcc agcacaccgg aaacgtgaaa atgtttcgcg aggaaaaacg aaccataggc
     2761 ggccagcgcc gttagggtca tctccacaac ttgatcgtct gtgcgcgcgg cgaacaattt
     2821 caacgcgaat ccaacgatga gtcctgccac gacgccgcca ccgaagacca gcagaaaatg
     2881 catcgtcgtg gccagccagc cgctgtcgct acccgtggcc aggcccagca ccacaacaaa
     2941 tgccaccgca gccgtgccat cattgagaag gctttccgcc tctaccagca ggcgcagccg
     3001 accatgcact ccggcctctt tgaaggttgc aatgaccgag actgggtcgg tcgcggaaat
     3061 gagcaccccg aatgcaccgg cggccgccca actccagccc accgcgtag
//
LOCUS       DATMRM010000037         9235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_16500, whole genome shotgun sequence.
ACCESSION   DATMRM010000037 DATMRM010000000
VERSION     DATMRM010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9235
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16500"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VNZ06_01030"
     CDS             complement(<1..640)
                     /locus_tag="VNZ06_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWW19360.1"
                     /translation="MCESAPAAETLSIESKALLDAAVDAVVLITHEGIVQAFNPSAVR
                     MFGYQAEEIIGGNVGRLMTDRDAAQHDAHLKRYLRTGETRIIGIGRAVSARRKDGSVF
                     PAFLSVGRLPGQGAPRFVGFLQDLTLRQEALAAIEAERERTARARDRMLHVARLATMG
                     EMVSGIAHELNQPLAAIANFAQACTRILASPDADLVDVKEALRQISAQALRAG"
     gene            complement(805..1233)
                     /locus_tag="VNZ06_01035"
     CDS             complement(805..1233)
                     /locus_tag="VNZ06_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19361.1"
                     /translation="MNSPLAHALPHHTVIWMDHRQAHIISFDSEREVGAQVHAHHTPE
                     HLHHKANTRGSGHAPVDQAFLRDIVEAVRHSGHILVTGPSGAKNDWCRFVELHHPQTF
                     QKIVGVQSLDHPSDGQLLALARKFMRAADRMYSQTDPHSL"
     gene            complement(1230..1526)
                     /locus_tag="VNZ06_01040"
     CDS             complement(1230..1526)
                     /locus_tag="VNZ06_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HWW19362.1"
                     /translation="MLSTYWSPAELQGVQRALTAQQRALLQQIDEIDANLERLGGELI
                     EVALALNRLRRGVFGVCTRCGQDIAASRLRAHPVAARCVSCQRSYEPMPTDGSL"
     gene            1806..3359
                     /locus_tag="VNZ06_01045"
     CDS             1806..3359
                     /locus_tag="VNZ06_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWW19363.1"
                     /translation="MEQWPTALLHPEAFPHPVKTLRLIETGISWVILTGEFAYKIKKP
                     LRTDFIDALTVERRKFLCEEEVRLNRRFCQDLYLGVVSIARDARGVGIDRMGSVVEHA
                     VMMRQFDQSQEMAHALAHGGVTADEMARLGTELARLHRHASASPLAAEYGSPSLIELQ
                     ILANFAPLQEALRGDTAMLKHLAVLEKWTQQAMTQARFWMIQRRSCLVRDCHGDLHAG
                     NVVFWQGRWQPIDCLEFNPALRWIDVISDLAFLFMDLRARARPELAAALLNAYLEESG
                     DYEGLRLLPLYAVYRALVRARVAALSQQSAVLRNYLRTAQVLTRTQHPFLILMHGVSA
                     SGKSWASARLARQLGALRIRSDVERHRIGGPAADAAGTMSVGERYTPDGLRTSYRRLQ
                     FCADSALSAGYPVIVDASFLDRERRGEFQALADRRGCSLLIVSCEAPLAVLEQRLRER
                     QARGADPSEATWEVLRGQWDALRAFDVCERGHVITIDTSESAAAEDTLRELCEQARLR
                     LRLSAEAAS"
     gene            3455..4261
                     /locus_tag="VNZ06_01050"
     CDS             3455..4261
                     /locus_tag="VNZ06_01050"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWW19364.1"
                     /translation="MRASNIMTRNVISVTPETTLKEAVTRMLEYGLSGLPVVSAGALV
                     GMITEGDLLRRSELGTEPHLSQWQQLVGGTQRQAINYVRTHGLRVGEVMSTQPIFVDP
                     DASLRKIVALMEARHVKRLPVLSAGHLVGMVSRADLLRVLMAESARGFCANCDDEQTV
                     RSIRAQIDREPWAPRAWVHVMVHDGVVELTGTVRHESERTALRVLAENTAGVKRVVDE
                     LIWIEPMSGAVVDLPKIVVDSPSMSVPVSTHGSVATEAKVPSWPNWNRSA"
     gene            4473..6002
                     /locus_tag="VNZ06_01055"
     CDS             4473..6002
                     /locus_tag="VNZ06_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWW19365.1"
                     /translation="MSTQFARAVLDAAPDAMVVVDTQGIVRYANKQVAALFGFEHDEI
                     IGFAVERLIPERFWGRHETHRKGYVEGMRARPMGQGLALFARRQDGSEFPVEVSLSPI
                     RDEGTSMVAAAIRDVTGRKKAEAELVVAREAAEAAREVADRANQAKSRFLATASHDLR
                     QPLQVLALLNGTLRRVVAPNVDAIEAVEQQELAIGAMSRLLNALLDIGKLESGAIKPD
                     PIDFAVAEIFRELRQEFAQIALNKGLSLEIEDSADIAHSDPSLVEQILRNLLSNAIKY
                     TRSGRVRVRSSRINSGIRIDVIDTGIGIPAEQIPYIYDEFYQVGVATNTTRDGYGLGL
                     SIVLRIVALLRADLDVKSEVNKGSTFSLVLPSSLRATAAPAAAPAASPAREVRRADAR
                     LLLVEDDPAVQMATKMLLKAGGYRVMSASTRADALQIAQTQPVDLLITDYHLGAGETG
                     TQVIAELRQILGPSLKAILVTGDTSSAVRELGSDPHLRVASKPIQAEQLLVLIATVLA
                     G"
     gene            6121..6564
                     /locus_tag="VNZ06_01060"
     CDS             6121..6564
                     /locus_tag="VNZ06_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWW19366.1"
                     /translation="MLVRDVLNADVICCGPDTTAAEAANLMREHHVGDVVVLAIGPPR
                     TPIGVVTDRDLAVEVMAKGIDPGGIQVRRFMRTPVVMAHDSEELEAVIERMRVNAVRR
                     MPVVDHEKLVGIVTMTDLLSALHVGISNVLSISDKAVRAERRLRR"
     gene            complement(6614..7243)
                     /locus_tag="VNZ06_01065"
     CDS             complement(6614..7243)
                     /locus_tag="VNZ06_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWW19367.1"
                     /translation="MHVRKLACVMGLAGVLNLGARHAASEEPPDGLPSLAARVAVATC
                     ATCHGPRGQSVQPKFPVLAGQHANYLLAQLQAFRAQTRGDPDAIGYMWGMAGPLSDEL
                     MGALADYYSAQTSRRGEQGDAAQIARGRQIYQNGDSNAGIPPCAACHGSGAAGTDTYP
                     RLAGQHVQYLLKQLRSFQNNMRNVAIMHGVAKGLNDADMRAVATYLQSL"
     gene            complement(7262..8689)
                     /locus_tag="VNZ06_01070"
     CDS             complement(7262..8689)
                     /locus_tag="VNZ06_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19368.1"
                     /translation="MSTHTIMTRYTRLLWILAAFGSLSFTRSVQAVPSFARQTGMACE
                     ACHTVFPELTHFGRMFKANGYTLDNLKPVRDVDSSRQELLSLSGLPPLSVMIEISETW
                     LDSPLPDGTGGPGHSQTSTTGFPQQLSLFYAGKVAPHVGAMVQLTYANDSGTLGIDNT
                     DIRYANLLLLPQDSSLVYGISLNNNPTVQDLWNSTPAWGFPFTSTNAAVSPLAAAQLD
                     GTLAQDVAGISAYIMWQEAVYAEFGVYRSAKQGVTNPITGAAGPLDGTISNVISGVAP
                     YGRLAYERQWQRHDLEVGVYGATFKLLPGGGTASAPASLSGPVNEFRDLAEDFQYQFA
                     GESHLFTLSGTHIHESMRLNASVLNASAANASDSLNTTRLTATYYYRRKIGASFQYFS
                     TTGTTDASLYAPGAAPGVVTSANGRPDTGGWISEINYLPWLNTKLTLQHTHYDRFNGG
                     TSNYDGFGRNASANDSTYLLFWIAF"
     gene            complement(8747..>9235)
                     /locus_tag="VNZ06_01075"
     CDS             complement(8747..>9235)
                     /locus_tag="VNZ06_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWW19369.1"
                     /translation="LAYDRKIIQIAGALQRVCSADLDLIYAETADALPRAPEGESPAQ
                     EQLRWLAHECQVLPQHTHVLDGTADHVLARFAASMRYDLLVMGHPAPRHRRFWHLDAP
                     PLINSLLERPMDVLVVNQECGELADALAGGTSIRVHERWVQRQDRSGKDVRSLKKTRY
                     LY"
BASE COUNT         1763 a         2706 c         2949 g         1817 t
ORIGIN      
        1 cacccgctcg caaagcctgg gctgaaatct gccgtaacgc ttccttgacg tccaccagat
       61 ccgcatcggg actcgccagt atccgtgtgc acgcctgcgc gaagttggcg atcgccgcca
      121 gtggctgatt cagttcgtga gcaataccgc tgaccatctc gcccatggtg gccaagcgtg
      181 ccacgtgcag catgcggtcg cgcgcgcgag cagtgcgctc gcgttccgct tcgatcgccg
      241 ccagcgcttc ctggcgcagg gtcagatcct gcaggaaacc cacaaagcgc ggtgcgcctt
      301 gtccgggaag acgcccgacc gagaggaacg ccgggaatac gctcccgtcc ttgcgacgcg
      361 cgctcactgc gcgaccgatg ccgatgatcc gggtctcgcc agtgcgcaga tatcgcttca
      421 ggtgagcatc gtgctgcgcg gcatcgcggt cagtcatcag ccggccgaca ttgccgccga
      481 tgatctcctc cgcctgataa ccgaacatcc gcaccgccga gggattgaac gcctgcacga
      541 tcccctcgtg cgtgatcagc acgaccgcat cgaccgcggc atccagcaag gccttggatt
      601 caatggagag ggtctcggca gccggcgcag attcacacac cctacaacta cctggtcttt
      661 acaaggccat cactataaac gagccgcgga tttgcgccgt aggtagtttc cgaaggcgcc
      721 aaatgtacgc tcgagcgcac ccagaccatt cgctcaccac ggcaagccac cgtgagtcac
      781 cggctcgagg ccccggggct accgctacag agaatgcgga tcggtctgcg agtacatgcg
      841 atcggccgcg cgcatgaatt tgcgggccag cgccagcagc tggccgtcgc tcggatggtc
      901 cagcgattgc acgcctacga tcttctggaa ggtctgcggg tgatgaagct cgacaaatct
      961 gcaccagtca ttcttggcgc cgctgggacc cgtcaccaga atgtggccgc tatgcctcac
     1021 ggcttcgacg atgtcgcgca gaaacgcctg atcgaccggt gcatgaccgc tgcccctcgt
     1081 gttggctttg tgatgcaggt gttcgggcgt gtgatgtgcg tggacttggg ccccgacctc
     1141 tcgttcggag tcgaaagaaa tgatgtgcgc ttggcgatga tccatccaga tgaccgtgtg
     1201 gtgcggcaaa gcgtgggcaa gcggagaatt catagagatc cgtccgttgg catgggctcg
     1261 tatgagcgct ggcaggagac acagcgcgcc gcaaccgggt gcgcgcgcag gcgcgaggca
     1321 gcaatatcct gcccgcagcg ggtgcagacg ccgaacacac cgcgacgcag gcggttcaat
     1381 gcgagcgcga cctcgatcag ttcgccgccc agacgctcca ggttggcatc gatctcatcg
     1441 atctgttgca acagcgctct ttgctgagca gtcagcgctc gctgaacccc ctgcagctcg
     1501 gcgggcgacc agtaggtgga aagcatggtt agaggcccct ggcggggtcc acgccatcgg
     1561 gccggcggcg gatcttcatg acgggttgct ccggagaggc gaaatcatct gaccggtcac
     1621 gttagagaaa gaaccgcctg acggcagccg tattgtccgc gcgaccctag gtacaactac
     1681 ctagtctcgt gggcggcgct cgatagataa gtagatgtgc gaatggcgcc ggtccgggtc
     1741 ccccccccat gctatggggc atgcgatcat cgacaaacac aacggctgcc cagagcaggg
     1801 cgcccgtgga acagtggcca acggcgctgc tgcatcccga ggcgtttcca caccctgtca
     1861 aaacgctgcg actcattgag actggcattt cctgggtgat tctcacgggc gagtttgcct
     1921 acaagatcaa aaagccgctt cgcaccgatt tcattgacgc tttgacggtt gaacgccgaa
     1981 agttcctttg tgaagaggag gtacgactca atcgacgctt ctgccaggat ctgtatctgg
     2041 gggtggtgag catcgcgcgt gacgcacgtg gcgtcggcat cgatcgaatg ggcagcgttg
     2101 tcgagcatgc ggtcatgatg cgccagttcg atcaatcgca ggaaatggcg catgcgctcg
     2161 cccacggtgg cgtgaccgcc gatgaaatgg cacgcttggg tacggagctc gcgcgtctgc
     2221 accgccatgc cagcgcctcg ccgctcgccg ccgagtacgg ttcaccttcg ctgatcgaat
     2281 tgcagatcct ggcgaacttt gctccgctgc aggaggcatt gcgcggcgac acggccatgt
     2341 tgaagcatct cgccgtactg gagaagtgga cgcagcaagc catgacgcag gcgcgattct
     2401 ggatgattca gcgtcgcagc tgcctcgtgc gcgactgcca cggcgatctg catgcgggca
     2461 acgtggtgtt ctggcaaggc cgctggcagc cgatcgattg cctggaattc aatcctgcat
     2521 tgcgctggat cgatgtgatc agtgacctcg cctttctgtt tatggacttg cgcgctcgcg
     2581 ctcgacccga gctcgccgca gcattgctga atgcctatct ggaggaaagt ggcgactacg
     2641 aaggcttgcg gctgctgcca ctctacgcgg tgtatcgcgc gctggtgcgg gccagagtgg
     2701 ccgcactctc acaacaatcg gcggtgctgc gcaattacct gcgcactgcg caggtgctga
     2761 ctcggacgca gcatccgttc ctgatactaa tgcacggggt gagcgcttca ggcaagtctt
     2821 gggcgagcgc ccggctggca cggcagctgg gcgcgcttcg cattcgctcg gacgtggagc
     2881 ggcatcgcat cggcgggccg gctgcggatg ccgcgggcac gatgtcggtg ggcgagcgtt
     2941 atactccgga cggcttgcgc acgagctacc ggcggctgca attctgtgct gatagtgcat
     3001 tgagcgccgg atacccggtg atcgtggatg cgagcttcct ggatcgtgag cggcgcggcg
     3061 aattccaagc gctggcggac agacgtggct gctcgctgct gattgtttcg tgcgaggcgc
     3121 ccctggccgt gctcgaacaa aggctgcgcg agaggcaggc ccgcggcgcc gatccttccg
     3181 aggcgacatg ggaggtgctg cgagggcaat gggacgcgct cagagccttc gacgtctgcg
     3241 agcgtggtca cgtcatcacg atcgatacgt ctgagtcggc cgcagccgag gacactcttc
     3301 gagagctgtg cgagcaggca cgccttcgtc tgcgtctgag cgcagaggcg gcgtcgtgac
     3361 atgcgctttc ggcaaagcgt atgggcagcg ggagactcct gcctatacat aggtaaggga
     3421 ccgtctaata cgattcaatc ggtgaggtat ccacatgcgc gcttccaata tcatgacccg
     3481 caatgtcatc agcgtgacgc cggaaacaac gctcaaagaa gccgtgacca ggatgctcga
     3541 gtacggactc agtggcctgc cggtcgtgag cgcgggtgcc ttggtcggca tgatcaccga
     3601 aggagacctg ttgcggcgta gcgagctcgg taccgaaccg catctgtcgc agtggcagca
     3661 gctcgtgggc ggtacgcagc gccaggcgat caactacgtg cggacccacg gtctgcgtgt
     3721 cggcgaggtc atgagtacgc agcccatctt cgtggatccg gatgcctcat tgaggaagat
     3781 tgtagcgctg atggaggcac gccacgtcaa gcgcctgccg gtactgtcgg caggccacct
     3841 ggtcgggatg gtcagtcgtg cggatttgct gcgtgtattg atggccgaat cggcacgtgg
     3901 attctgtgcc aactgtgatg atgagcagac cgtgcgcagc attcgcgcgc agattgatcg
     3961 agagccatgg gcgccgcgcg cgtgggtgca tgtgatggtg catgatggcg tggtcgagct
     4021 gacaggcacc gtgcggcatg agtccgagcg caccgcgctg cgcgtgctcg ccgagaatac
     4081 ggccggtgtc aagcgagtcg tggatgagtt gatatggatc gaaccgatgt cgggcgctgt
     4141 agtggacttg ccgaaaattg tcgtagattc cccgagtatg tcggtccctg tctccacgca
     4201 tggatccgtc gcgactgagg ccaaggtgcc ctcctggccg aactggaatc gatcggcctg
     4261 aaaccgcctg gatccgtcag gaccgcgagc tgtcttgcgg actagttgtt gaggactgcg
     4321 agggccacgg ggggcgtggc ctgatccact tttgccttcg gcatatgctg acaagtgtgc
     4381 tgatacttgt aagggaggca gactggcccc attttggact tctggtgcag aatgcgtcga
     4441 agtcgataaa ggcgatagag atcatgttgt cgatgtccac gcagtttgca cgggcggtgc
     4501 tggacgcagc acctgatgcc atggtggtgg tggacactca gggcattgtg cggtacgcga
     4561 acaaacaggt ggccgcgctt ttcggcttcg agcacgacga gatcatcggt ttcgcagttg
     4621 aacgattgat tcccgagcgc ttctggggcc gtcacgagac tcaccgcaaa ggctatgtcg
     4681 aaggaatgcg ggcgcgcccg atgggacagg gtctggcact gttcgcacgg cgtcaggatg
     4741 gctcggaatt tccggtcgag gtgagcctaa gtccgattcg ggacgaaggt acatcgatgg
     4801 tggcggcggc gattcgtgac gtcaccggcc gcaagaaagc cgaggcggaa ctcgtcgtcg
     4861 cacgcgaagc ggccgaggcg gcgcgtgaag tcgccgatcg cgccaatcag gccaagagtc
     4921 gatttcttgc cacggcaagc catgatctgc gccaaccgct gcaggtgctg gccttgctca
     4981 acggcacgct tcgccgagtc gttgccccca acgtcgatgc catcgaggcg gtggagcagc
     5041 aggagctcgc cattggcgcg atgtcgcggc tactgaatgc cttactggac atcggcaaat
     5101 tggaatccgg tgcgatcaag ccggacccca tcgacttcgc ggtcgcagaa atattccgcg
     5161 aactgcgcca ggaattcgcg cagattgcgc tgaacaaggg tctgagcctg gagattgagg
     5221 actcggcgga cattgcgcac agcgacccct cgctggtcga gcagatcctg cgcaatctgc
     5281 tgtccaacgc gatcaaatac actcgcagcg gcagggtacg ggtgcgatcg tcacgcatca
     5341 atagcggcat ccgtattgat gtgatcgata cggggatcgg aattccggcg gagcagatac
     5401 cctatatcta tgatgagttt tatcaggtag gtgtggccac caatacaact cgggacggct
     5461 acggcctggg cctgagtatt gtattgcgca tcgtggcgct gttgcgtgcc gaccttgatg
     5521 tcaagtccga ggtcaacaag ggcagcacgt tcagcctggt gctgccctcg agtctgcgag
     5581 ccaccgcggc accggctgcc gcgcccgctg cgtcacccgc gcgcgaggtt cggcgggccg
     5641 atgccagact gttgctggtc gaagacgatc ctgccgtgca gatggccacg aaaatgctgc
     5701 ttaaggctgg aggctatcgc gtcatgtccg catccactcg cgccgacgca ctgcagattg
     5761 cacaaactca gccggtcgat ctgctcatca ccgactacca tctgggtgcc ggtgagaccg
     5821 gcactcaggt cattgccgag ctgcgtcaga tcctggggcc atcactgaaa gcaatcttgg
     5881 tgactgggga tacgtcctct gcagtgcgtg aactgggcag cgacccgcac ctgcgggtcg
     5941 ccagcaagcc catccaggcg gagcagctat tggttttgat tgccaccgtg cttgccggct
     6001 aggttgtcag aatccctcgg aaagtacgga tgcgatgccg gcgggccgcc gcaatactga
     6061 cattcaacac ataggagtcg cctgagtgag cggcccttcg gacaagcagg agaaaggatc
     6121 atgttggtaa gggacgtgtt gaatgcggat gtcatttgct gtggacctga cacgaccgcg
     6181 gcggaggcgg ccaatctgat gcgcgaacat catgtgggag atgtcgtggt attggcgata
     6241 gggccgccgc gtacgcccat tggcgtggtt accgatcgcg atttggcggt ggaggtgatg
     6301 gcaaagggca tcgatccggg tggtatccag gtgcgccgct tcatgcgaac accggtcgtc
     6361 atggcgcatg attccgagga actcgaagca gtgatcgaac gcatgcgtgt caacgcagtg
     6421 cgtcgtatgc cggtcgtgga ccacgaaaag ctggtgggaa tcgtgaccat gacagacttg
     6481 ctgagtgcgc ttcacgtggg aatcagcaac gtgctgagca tttccgacaa ggccgtccgc
     6541 gccgagcgac gcttgcgccg ctaacgttga cgcggtcgcc gcgatggcgc ggcggcagca
     6601 ttgctgcccc gctctacagg gattgcaaat aggtcgcgac cgcccgcatg tcagcgtcat
     6661 tgagcccctt tgccacgcca tgcatgatgg caacattgcg catattgttc tgaaacgaac
     6721 gcagctgttt caataggtat tggacatgtt ggccagcaag ccgcggatag gtatctgttc
     6781 ctgcggcgcc ggagccgtga caggccgcgc agggcggtat ccctgcattc gaatctccgt
     6841 tctgatagat ctgcctgcct cgcgcaatct gggcggcatc gccttgctcg ccccgacgtg
     6901 aagtctgagc gctgtagtag tctgcaagcg ctcccatgag ctcgtcagac agtgggccgg
     6961 ccatgcccca catatatccg atggcgtcag ggtcgccgcg agtctgtgcc ctgaaggctt
     7021 gcagctgtgc aagcagatag ttggcatgct gacccgccag caccggaaat ttcggctgga
     7081 cgctttgacc gcgcgggccg tggcacgttg cgcaggtcgc caccgcaaca cgagctgcga
     7141 ggcttggcaa tccatccggt ggctcctcgc tggcggcatg tcgtgccccc agattcagca
     7201 cgccagccag tcccatgacg caagcaagct ttcgcacgtg catgtcgtaa ctctcctcgt
     7261 ctcagaacgc aatccagaac agcagatacg tcgagtcgtt cgccgatgcg ttgcgtccga
     7321 aaccatcgta gttgctggtg ccgccgttga atcgatcgta gtgggtgtgc tgcagcgtca
     7381 acttcgtgtt aagccagggc aagtagttga tctcggagat ccagccgccc gtgtcgggcc
     7441 ggccattggc cgacgtcacg acaccgggcg ctgctccggg cgcgtagaga ctcgcatcgg
     7501 tcgtccccgt ggtcgagaaa tattggaagg acgcgccgat cttgcggcgg tagtagtacg
     7561 tggctgtcaa gcgcgtcgtg ttcagcgaat cgctggcatt ggccgcgctc gcgttcagta
     7621 cgctggcgtt cagacgcatc gattcgtgaa tgtgcgttcc gctcagcgtg aagaggtgag
     7681 attcacctgc gaattggtac tgaaagtctt ccgctagatc cctgaattca ttgaccggtc
     7741 cgctcaatga ggccggagca ctcgccgtgc cgcctcctgg cagcagcttg aaggtggctc
     7801 cgtagacgcc tacctccagg tcatgacgtt gccactggcg ctcgtatgcg agtcgtccgt
     7861 agggggcgac cccgctgatg acattgctta tcgtaccgtc cagcggtccg gccgcaccgg
     7921 tgatcgggtt cgtgacaccc tgcttggcgg agcgatagac gccaaactct gcgtacaccg
     7981 cttcctgcca catgatgtag gcgctgatcc ccgccacatc ctgcgcaagt gtgccgtcga
     8041 gctgcgccgc cgccaggggc gatacggccg cattggtgct ggtaaacgga aacccccagg
     8101 cgggggtgct attccacaga tcctgcaccg tcggattgtt gttgaggcta atgccgtaga
     8161 cgagactact atcctgcggc aggagaagga gattggcata gcggatatcg gtgttgtcga
     8221 ttccaagggt tcctgagtcg ttggcatagg tcagttgaac catggctccg acatgagggg
     8281 ccaccttgcc cgcatagaac aggctgagtt gctgaggaaa acccgtggtg ctggtctgcg
     8341 agtgacccgg gccacctgtg ccgtccggca gaggagagtc cagccaggtc tccgagatct
     8401 caatcatcac cgataaggga ggcagtcccg acaacgacaa tagctcctgc cgcgaactgt
     8461 caacgtcgcg caccggcttc agattgtcca gcgtgtagcc gtttgccttg aacattcggc
     8521 cgaagtgagt gagttccgga aacaccgtat ggcatgcctc gcatgccatg cccgtctgcc
     8581 gtgcaaagct gggtaccgcc tgaacggacc tggtgaatga aaggctaccg aaggccgcga
     8641 gtatccatag cagtcttgta tatcgcgtca tgatcgtatg ggtactcacc ggcctctccc
     8701 ctggttgatg cgggggagct tgacgtttac cgatcgacgg cagcaatcag tacaaatacc
     8761 tagtcttctt gagggagcgg acgtctttgc cgctacggtc ctgacgttgc acccatcgct
     8821 catgcacacg tatggaggtg ccaccggcca atgcgtcggc caactcaccg cattcctgat
     8881 tgacgaccag tacatccatg ggtcgttcca atagcgaatt gatcaatggc ggcgcgtcaa
     8941 ggtgccaaaa gcgccgatgc ctaggcgccg gatgccccat cactagcagg tcgtagcgca
     9001 tcgaggctgc gaatctggcg agcacgtgat ccgcggtgcc atcaagcaca tgagtatgct
     9061 ggggcagcac ctggcactcg tgtgccaacc agcgaagttg ttcctgtgcg ggagactcac
     9121 cctccggtgc cctgggtaag gcatcggcgg tttcagcata gatgagatcc aggtctgcgc
     9181 tgcagacacg ctgcaaggcg cccgcgatct ggatgatctt gcgatcatag gccag
//
LOCUS       DATMRM010000038         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4359025, whole genome shotgun sequence.
ACCESSION   DATMRM010000038 DATMRM010000000
VERSION     DATMRM010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4359025"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VNZ06_01080"
     CDS             complement(<1..1194)
                     /locus_tag="VNZ06_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWW19370.1"
                     /translation="MPLENFHPAVARWFSSQFPSPTAPQLAAWPAIAAGGHVLIAAPT
                     GSGKTLAAFLAAIDSLVRQAVDGHLADITQVVYVSPLKALSNDVQRNLEQPLTGIAAQ
                     LRAMGLACPAIRAQVRTGDTAQADRAAMRRSPPHILVTTPESLYLLLTSESGRKMLSN
                     TRTVIIDEIHAVAPSKRGAHLALSLERLEGLCERRLQRIGLSATQKPIEEVARFLVGS
                     AAQERDTTIVDNGHVRGRDLALVLPDAPLEAVMSNEVWENLYDQLAHLSADHRTTLIF
                     ANTRRMVERVTRHLSERIGEDNIAAHHGSLAKEQRLDAEQRLKSGALRVLVATASLEL
                     GIDIGDVELVCQLGTPRSISTFVQRVGRANHAVNGVPKGRLLPLSRDDLVECTALLDA
                     VRRGEL"
     gene            1235..1762
                     /locus_tag="VNZ06_01085"
     CDS             1235..1762
                     /locus_tag="VNZ06_01085"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWW19371.1"
                     /translation="MSTYMLFPTGVGPCGLAWGPNGIVGVQLPESNASKTLARLLHRL
                     GGDASPGQAPPDVQQVIDDIVALLDGERRNLQYARLDMDRVPELFRRIYAVVRRIPAG
                     QTLSYGEVATRCGDTDARTVGQAMARNPFPIVVPCHRVAAANGKGGGFSAYGGVTTKL
                     RLLALEGAGTLSLPW"
     gene            1817..>2108
                     /locus_tag="VNZ06_01090"
     CDS             1817..>2108
                     /locus_tag="VNZ06_01090"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWW19372.1"
                     /translation="MNAMKLAVTAAAVSVAFATTAFAAPTTYKTDANHTFVRFSYNHM
                     GFSTQESRFNKVSGTITFDPAARSGSVDVSIDTKSVDTGSDLFNQHIQGADFL"
BASE COUNT          385 a          652 c          676 g          395 t
ORIGIN      
        1 gagttcaccg cggcgcaccg catcgagcag cgcggtgcat tccaccagat cgtcacggct
       61 cagcggcagc aagcgccctt tgggaacgcc gttgacggcg tgattggcgc ggccaacgcg
      121 ctgtacgaag gtcgatatcg agcgcggcgt tccaagctga cacaccagct cgacgtcgcc
      181 gatatcgatg cccagttcca acgaggccgt ggcaaccagc acacgcagcg caccgctctt
      241 caaacgttgc tcggcatcca gccgctgctc cttggcaaga ctgccgtggt gcgcagcgat
      301 gttgtcctca ccgatgcgct cgctcaagtg gcgagtgacg cgttccacca tgcgacgcgt
      361 gttggcgaag atcagtgtgg tgcgatgatc cgcgctcaag tgcgccagct gatcgtagag
      421 gttctcccag acctcgttcg acatcaccgc ttcgagcgga gcgtccggta gtaccagcgc
      481 cagatcccgg ccgcgcacgt gcccgttgtc aacgatggtt gtgtcgcgct cttgtgcggc
      541 gctgccgacc agaaatcgtg caacctcttc gatgggcttt tgcgtggccg acaggccaat
      601 gcgctgcagg cgacgctcgc acagtccttc gagtcgctcg agcgacagcg ccagatgtgc
      661 gccgcgcttg ctcggtgcga ctgcgtggat ctcatcgatg atgaccgtgc gcgtgttcga
      721 gagcattttg cggcccgact cactggtcag cagcaggtaa agggactcgg gcgtcgtcac
      781 cagaatatgg ggcggactcc tgcgcatcgc ggcgcggtcg gcctgggcgg tatcgccggt
      841 gcggacttgg gcgcgaatgg ccggacaagc caagcccatg gccctcagtt gggctgcgat
      901 gcctgtcagc ggttgctcca gattgcgttg aacgtcgttc gacagcgcct tgaggggcga
      961 tacgtagaca acctgtgtga tatcggccag atggccgtcg acggcctgcc gcaccagtga
     1021 atcgatggcc gcgagaaagg cggccagcgt ctttcccgaa ccggtcggcg ccgcgatcaa
     1081 gacatgtcca ccggccgcaa tcgccggcca ggccgcgagc tgcggtgcgg tcggcgaggg
     1141 aaattggctg gaaaaccagc gcgcaacggc tggatgaaag ttctccaggg gcatgatctt
     1201 gattctactc ccggacgcta gaatcatggg tgacatgagc acctatatgc tgttcccgac
     1261 aggggttggt ccgtgcggac ttgcgtgggg tccgaacggc atcgttgggg tgcaactgcc
     1321 ggaatccaat gcgtcaaaaa cgctggcgcg actgctacac aggctggggg gcgatgcgag
     1381 cccgggacaa gcgccgccgg acgttcagca ggtcatcgat gacatcgtgg ctctgcttga
     1441 cggtgagcgg cgcaatcttc agtacgcccg gctcgatatg gaccgggtgc cggagctgtt
     1501 tcggcgcatt tacgcggtcg tccgccggat tcctgccggt cagaccctct cctacggcga
     1561 ggtggccacc cgctgcggag ataccgacgc aagaaccgtc ggccaggcca tggcgcgtaa
     1621 tccgtttccg atcgtcgtgc cgtgccaccg ggtggcggcg gcaaacggca agggcggcgg
     1681 tttttccgct tacggcggcg tgaccacgaa gctgcgcctg ctggcgctgg agggtgccgg
     1741 cacgctgtcc ctgccgtggt agcccaggat aagatgcgca tcccctagaa cctcaaaaat
     1801 cccggaagga gtcccgatga atgcgatgaa acttgcggtc acggcggcag ctgtgtcggt
     1861 tgcgtttgca accactgcat ttgccgcgcc caccacttac aagaccgatg ccaatcacac
     1921 gtttgtgcgc ttttcgtaca accacatggg cttctccacg caggagtcgc gcttcaacaa
     1981 agtaagcggc acgatcacat tcgatccggc agccagaagc ggttccgtgg acgtttccat
     2041 cgacaccaag tcggtcgaca ccggttcgga tctgttcaat cagcacattc agggcgccga
     2101 ctttctcg
//
LOCUS       DATMRM010000039         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_17118, whole genome shotgun sequence.
ACCESSION   DATMRM010000039 DATMRM010000000
VERSION     DATMRM010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17118"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1475)
                     /locus_tag="VNZ06_01095"
     CDS             complement(93..1475)
                     /locus_tag="VNZ06_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HWW19373.1"
                     /translation="MRQVIPWIAVVVCLTAEVPALAADHDLSVAIYNDDLALVQDHRT
                     VNLTGGRQRIEFQNVSAKIRPETVSLAASELTILEQNFDFDLLTPAKLMEKAVGQEVT
                     LVRVNPATGAETREKAQVLATNGGVVLKIGDHIEVLRDDGLPIRVIFDKVPDNLRAKP
                     TLSITVSGARGGSLPVTLSYLTPGLGWRADYVALFNEADSKIDVQGWVTLTNSSGTTY
                     DDAKTLLVAGSPARADTNGNFPYRPTRRPTLQSAGTESGTRERLADYYLYPLAERTTI
                     ANLQTKQVSFLDVHAVPAEHAYEYRNEWLGTTDVPQSAKSVYRFSTSAHAGLGDQLPA
                     GILRFYMRDKRGDPQFIGESRIDHTPMGSTLSLATGDAFDVKVKATVEKRTLLAPFKW
                     QSDMRYELTNALSQPVTVTLLQAGLWGDTRIRSESQKSTRRSAEVAEWAVAVPANAKS
                     ELTASFESRY"
     gene            2011..>2561
                     /locus_tag="VNZ06_01100"
     CDS             2011..>2561
                     /locus_tag="VNZ06_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19374.1"
                     /translation="MAEIRDAFEAYSSGELAEYELRSALRAEIQTQPGAVARYAAMAA
                     ALRRRNLISAELEAAVVSDMNAVVEAQIMASQPVRGADSEDPTRAGPHEPSIGEPPSP
                     SALSEPPSSTRRAPTLDVSRPLTPLPSTASGSSHSANRGGAWDSNERLAEPETPVTVG
                     MVLRERFELVEELGRGGMGVVYKA"
BASE COUNT          487 a          765 c          803 g          506 t
ORIGIN      
        1 ggtccccgcg gtgatgtcac ccgcactcgc ccccagtgga atcaacgccg ccgttgcgag
       61 cagccacgga cgaatcgagc cgcgcatgct ggtcagtaac gcgactcgaa ggacgcagtc
      121 agctccgact ttgcattcgc gggtaccgcg acggcccact cagccacctc ggcgctacga
      181 cgcgtgctct tctgactttc ggacctgatc cgggtgtcgc cccacagtcc agcctgaagc
      241 agtgtcaccg tcaccggttg cgacagcgca ttcgtgagtt catagcgcat gtcggattgc
      301 cacttgaatg gtgcgagcaa cgtgcgcttc tccacagtcg ccttgacttt gacgtcaaag
      361 gcatcgcccg ttgcgaggga cagcgttgaa cccatcgggg tgtggtcgat tcgactctca
      421 ccgatgaact gcgggtcgcc acgcttgtcg cgcatgtaga aacgcaggat cccggccggc
      481 aactggtcgc caaggcccgc gtgcgcgctg gtcgagaatc ggtagaccga cttggcgctt
      541 tgcggcacgt cggtcgtccc gagccactcg ttgcgatatt cataggcatg ctctgcgggg
      601 acggcgtgta catccaggaa actaacctgc ttggtctgca ggtttgcgat cgtggttcgc
      661 tcagcgagcg gatagagata gtaatcagcg agccgctcgc gcgtgccgct ctccgtgcct
      721 gcgctctgca acgttggacg tctcgtcggg cgatagggaa agttgccatt tgtatcggca
      781 cgggcgggtg agcccgcaac caacagggtt ttcgcatcgt cataggtcgt cccgctcgag
      841 ttcgttagcg tgacccagcc ctgcacatcg atttttgagt ccgcttcgtt gaacagcgcg
      901 acgtagtcgg cgcgccagcc cagtccaggc gtcagatagc ttaaggtgac gggtagcgag
      961 ccgccgcgtg caccgctgac ggtaatcgag agcgtcggtt ttgcgcgcag attatccggg
     1021 actttgtcga agatgacgcg aataggcaga ccatcgtcgc gcagtacttc gatgtgatcg
     1081 ccgatcttga gcaccacacc gccgttggtc gccagcactt gcgctttctc gcgggtttcc
     1141 gctccggtcg ccggattgac tcgaaccagg gtgacttcct ggccaacggc cttttccatg
     1201 agtttcgccg gcgtcagcag gtcgaaatcg aaattctgtt cgaggatcgt cagctcactg
     1261 gctgccagcg agactgtctc aggtcggatc ttggccgata cgttctgaaa ttcgatccgc
     1321 tggcgcccgc ctgtcaggtt gacggtgcga tgatcctgaa ccagagcgag gtcatcgttg
     1381 tagatcgcta cggaaaggtc gtgatctgcc gcgagcgcag ggacctctgc agtgaggcag
     1441 accacgaccg cgatccaggg tatcacttgg cgcataacgc acctccccgc tgtgcgcgct
     1501 gttatcactg agcgcctcat gataatcaca tacgtacggg tgcgcccgga cggctcggaa
     1561 cggcgacggc gaccacctgt gtcgcagtgc cggaaagtga aatctgctgg cggccgatgt
     1621 cccgagaggc gcaccagcaa cccgtcggga tttcaagacg gtacgctgtt gtcttcaggt
     1681 cctctggtgg agacggccgc cgtttcgtgc ttgaatctat gtctttcagt agtttgctgc
     1741 gatgtctgaa tgcaaagctg gaacttgccg gtgacataac gagacccatc gtgcctcgac
     1801 ccactggcgg aggggcgcga aaacttgtgc ggaattcatg ccgccagcta aagagaccgc
     1861 tggcatcccc taggcactaa gataagatca atcatccgct ttggtgaggt gacacgcgac
     1921 gcatagcgcg cgtgttgcag cggatttgat gagaccaaca acaacattcg acggcactgg
     1981 attgatcgac agagggatgt gcggatgggc atggctgaga tcagggatgc gtttgaagcc
     2041 tactcctcgg gcgagttagc cgaatacgaa cttcgaagcg cactgcgcgc cgagatacag
     2101 acgcagccgg gggcggtggc ccgatatgcg gccatggcgg ccgcccttcg tcgtcgcaac
     2161 ctgatcagcg cggaacttga ggctgcggtg gtctccgaca tgaatgcggt cgtggaagcg
     2221 cagataatgg cttcgcagcc cgtgcgcggt gcagacagcg aagatccgac cagagccggt
     2281 ccgcacgaac cctcgatcgg cgagccgcca tcgccgtcgg cgctcagcga accaccctcg
     2341 agcacgcgtc gagctccaac cctggatgtt tcgcgtccgt tgacgccgct gccgagcacc
     2401 gcatcgggat cgtctcacag cgcgaaccgc ggcggtgcct gggatagcaa cgaacgactg
     2461 gcagaaccgg aaaccccggt gacggtcggt atggtgctgc gcgagcgctt cgagctggtc
     2521 gaagaactcg gtcgcggcgg catgggcgtt gtgtacaagg c
//
LOCUS       DATMRM010000040         5554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_643861, whole genome shotgun sequence.
ACCESSION   DATMRM010000040 DATMRM010000000
VERSION     DATMRM010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643861"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="VNZ06_01105"
     CDS             <1..1451
                     /locus_tag="VNZ06_01105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWW19375.1"
                     /translation="LAGARLHGDPNPIGEAAGRDLLYHSPASYDEILTLARLGMLPFL
                     LVLLISTWSWTRRWYGEAASLTATLFLVATPVILGHASVVALDVPVTAMSMLTLYVLL
                     RWFEAPTWRAALCLGLSAGLAMASKISAIPFLGVSLAGLLLLRAVLLARAPLSWQLPR
                     RIGTAAVALLLSVAVLLGMYGPRRVYLTDAQHTPSRSLDFLAGSHGVLHELAYRLAAQ
                     VPLPLGFKMVPLSILGVEFHNTHGHNSFLLGQTALNGWWYFYPVALAVKTPLPLLLLG
                     LAGLALMAVRGWRTANLYRLAPAACFASILLFACLYSHINIGVRHVLIAYPLLAMGAG
                     NLIVTLWQRWRSRAGHAVLAGLVLWEAATLAFTYPDYLAYFNLIGEPHPERILVDSDL
                     DWGGQDLRRLEKVLAVRGVQQFWLGYRGTADLSREPLPHFQSLLPGQPVTGWIAITLL
                     TLQENQAGYGWLDRYQPEHVGKSFYLYHIPGN"
     gene            complement(1476..3323)
                     /locus_tag="VNZ06_01110"
     CDS             complement(1476..3323)
                     /locus_tag="VNZ06_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HWW19376.1"
                     /translation="MIRPLAALAALAAFSLSGVLFAADQSVRGVAHPQLWPRAHSVGL
                     VSADSERFISELMGRMSLEDKVGQIIMANIDSITPEELRSYPLGAVLAGGAVPPLGGD
                     DRIPAVWLDTTRAFHDVALEVRPGHTSIPLLFAVDAVHGNNAVEGATLFPHNIGLGAT
                     RDARLVQRIGEATAQETLAVGMDWAFAPTLAVPQDLRWGRAYESYSQDPAVVATLGAA
                     LVLGLQGSHVAATAKHFIGDGGTTGGIDQGDTDVSEQQLIQRHTPGYLSAINAGVMTV
                     MASYSSWQGSKLHGNRSLLTDVLKQRLGFQGAVVGDWNGHAQLPGCSSTSCPQALNAG
                     IDMLMAPEGWKQMYDNVLAQVRRRQIPAARLEDAVRRVLRVKVKLGLFEASRPLEGQV
                     QLIGSPEHRALAREAVRKSLVLLKNRARVLPLRAGAHVLVAGDAADDIGRQCGGWTLT
                     WQGVENDNDAFPYAQSIYQGLREALLAQGGGAELSADGRYERKPDVAIVVFGEHPYAE
                     SLGDLKSIDYQPGSRQDLALLRRLHAARIPVVAVLLSGRPLVMNQEIDTADAFVAAWL
                     PGTQGGAVADVLIGDARGEPRYAFSGELSFAWPGSATGVRYPIGFGLRY"
     gene            complement(3336..4238)
                     /locus_tag="VNZ06_01115"
     CDS             complement(3336..4238)
                     /locus_tag="VNZ06_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HWW19377.1"
                     /translation="MRIFHPSRALQLLIACVTMGIGQDAQAWGDEGHAVVALIAEHYL
                     QPTVRERVRLLLEADSTGLVAERSMAGESVWADRYRDSDRDSTRARYEATRRWHYVDI
                     ELDDPSVPHACFGRPLLPAGTPASAGAAGDCVVDKIDQFQAELSDTKTSTGERRIALQ
                     FLLHLVGDLHQPLHAADQHDQGGNLEQVDAPGLSPQNLHRYWDVEFVQALGVDPRNVA
                     SMLIDQISPQELASWQRGSASDWAMESFAMARRTSFGLLPAAYDTHRYHLSAAYMQSA
                     AEAVRLQLKRAGVRLALILNRSLG"
     gene            4307..>5554
                     /locus_tag="VNZ06_01120"
     CDS             4307..>5554
                     /locus_tag="VNZ06_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWW19378.1"
                     /translation="MRRGKTALLRNWPAALFALVFIVSLCPLTSHARDGILSRPQGVD
                     QWRTHRIIVKWRSEGVAAVQMNRVEDRAARLSRTSGIHFSPARNLYGRTDVMLLDHIP
                     AHSEMQDILARLNADPGIEYAEPDEVRFLQDFPITTQMPNDSHFYASTDPMNAGSPDL
                     AIGPWVGQWYLLPSSSTTPSAISATTAWATATTLGKYVVVAIIDSGIAQDHEDLAQNM
                     LTPGYDFVSCDEGNFASTTTTALGATQGINQCTASGSAETYFFSNENSNWYADGTDPG
                     DFIDTADLALPEFANLGCKQTVASSWHGTKVAGVLGAVANNGKGIAGVAPAVTMISVR
                     VIGSCRGALLSDIASAILWAAGQSVPISTGNISSSPKANIINLSLGANTPCAATEQDA
                     INAAVNAGVLVVAAAGNEGGAIDA"
BASE COUNT          930 a         1906 c         1732 g          986 t
ORIGIN      
        1 acctggcggg cgctcgcctt cacggtgatc ccaacccgat aggggaagcg gcgggccgcg
       61 acctgctcta ccactccccg gccagctatg acgaaatcct gacgctggcc cggctgggga
      121 tgctgccgtt tctgctggta ctgctgatct ccacgtggag ctggacacgg cgctggtacg
      181 gcgaggcagc aagcctcacc gcgacgctgt ttctggtggc aacccccgtg atcctcgggc
      241 atgcctcggt cgtggcgctg gacgtgccgg tcacggcaat gagcatgctg acgctctatg
      301 tgctgttgcg ctggttcgag gcgccgacct ggcgcgcagc gctgtgtctg ggactgtcgg
      361 caggacttgc gatggcgagc aagatctcgg cgatcccgtt cctgggtgtc tcgctggcag
      421 gcctgctgct gctgcgagcc gtgctgctcg cgcgggcgcc attgtcctgg cagctaccgc
      481 ggcgcatcgg caccgcggcg gtggcgctgc tgctgtcggt cgcggtgctg cttggcatgt
      541 acggcccacg ccgcgtgtac ctcaccgacg cgcagcacac gcccagccgc tcgctcgact
      601 tcctggccgg ttcgcacggc gtgctgcatg agcttgccta ccggttggcg gcgcaggtcc
      661 ccctgccgct gggattcaag atggtgccgc tgagcattct cggcgtcgag tttcacaaca
      721 cccacggtca caactcgttt ctgctcggtc agaccgccct caatggctgg tggtacttct
      781 atccggtggc actggcagtc aagacgccgc tgccgctgct gctgctgggg cttgcggggt
      841 tggcgctgat ggcggtgcgc ggctggcgca ctgcgaacct gtaccggctg gcacccgcgg
      901 cgtgcttcgc atcgatcctg ctgttcgcct gtctctacag tcacatcaac atcggggtca
      961 ggcatgtcct gatcgcctat ccgctgctcg caatgggtgc cggcaatctg atcgtgacgc
     1021 tgtggcagcg ctggcgctcg cgcgcaggcc acgcggtgct cgccgggctg gtgctatggg
     1081 aagccgcgac actggctttc acctatccgg actacctggc ctacttcaac ctcatcggcg
     1141 agcctcaccc cgagcgcatt ctggtggact cggatctgga ctggggcggc caggacctgc
     1201 gacgtctgga gaaggtgctt gcagtgcgcg gcgtgcagca gttctggctc ggctacaggg
     1261 ggaccgcgga tctgagccgc gaaccgctac cgcacttcca gtccctgctt cccggtcagc
     1321 cggtcaccgg ctggatcgcc atcacgctgc taacgctgca ggaaaatcag gccggctatg
     1381 gctggctcga tcgctatcag cccgagcacg tgggcaagtc cttctatctc tatcacattc
     1441 cgggaaactg aattttctta gatatatctt atatcttagt accgcaagcc aaatcctatc
     1501 ggatagcgca cgcccgtcgc cgaacccggc cacgcgaacg acagctcacc cgagaaggca
     1561 tagcgaggct caccgcgcgc gtcgccgatc agtacgtccg ccactgcgcc gccctgcgta
     1621 cccggcaacc atgccgccac gaacgcatcg gcggtatcga tttcctgatt catgaccagc
     1681 ggtcggcccg acagcaacac cgccaccacc ggtatgcgcg cagcgtgcag ccgtcgcagc
     1741 agcgccaggt cctggcggct gccgggctga tagtcgatgc tcttcagatc gcccagcgac
     1801 tcggcgtacg ggtgctcgcc gaacaccacg atcgcgacat ccggcttgcg ctcgtagcgg
     1861 ccgtccgcac tgagctcggc gccgccgccc tgcgcgagca atgcctcgcg caggccctga
     1921 tagatggact gcgcgtacgg gaaggcatca ttatcgttct ccactccctg ccaggtcagg
     1981 gtccagcctc cgcattggcg tccaatgtca tcggccgcat cgcccgccac cagaacgtgc
     2041 gcgcccgcgc gcaagggcag cactcgcgcc cgattcttca gcagcaccag cgactttctc
     2101 accgcctcac gcgccagcgc acggtgctcg ggggaaccga tgagctgtac ctgtccctcc
     2161 agtggtcgtg aggcttcgaa cagacccagc ttcactttca cgcgcagcac ccgcctcacg
     2221 gcatcctcga gccgcgcagc cggaatctgc cggcggcgca cctgtgccag gacgttgtcg
     2281 tacatctgct tccagccctc gggtgccatg agcatgtcga tgccggcgtt gagcgcctgc
     2341 gggcaactcg tgctgctgca accgggtagc tgtgcatggc cgttccagtc accgaccacg
     2401 gcgccctgaa agcccaggcg ctgtttgagc acatcggtca gcaggctgcg attgccgtgc
     2461 agcttgctgc cctgccagct cgaataggac gccatcacgg tcatcacacc cgcgttgatc
     2521 gcggataggt agccgggcgt gtgccgctgg atcagctgct gctcactgac atcggtgtcg
     2581 ccctgatcga taccgcctgt ggtaccgccg tcgccaatga aatgctttgc ggtcgccgcc
     2641 acgtgcgagc cctgcagacc gagcaccagc gctgcgccga gcgttgccac cactgccggg
     2701 tcctgtgagt agctttcata ggcgcggccc cagcgcagat cctgcggcac cgcgagtgtc
     2761 ggtgcgaacg cccagtccat gccgaccgcc agcgtctcct gcgccgtggc ctcaccgata
     2821 cgctgcacca gccgcgcatc gcgtgtggca cccagtccta tgttgtgcgg aaagagggtt
     2881 gcgccctcga cagcgttgtt gccgtggacc gcgtcgaccg caaacagcag cggaatcgag
     2941 gtgtgcccgg gccgcacttc cagcgcgacg tcgtgaaacg cgcgcgtggt atcgagccac
     3001 accgcaggaa tgcgatcatc gccgccgagc ggcggcaccg caccgcccgc cagcaccgct
     3061 cccaacggat agctgcgcag ctcttcgggc gtaatactgt cgatgttggc catgatgatc
     3121 tggccgacct tgtcctccag actcatccgt cccatcagct cgctgatgaa acgctcgctg
     3181 tcggcgctga ccagtccgac actgtgagcg cgaggccaca gctgcggatg ggcgacaccg
     3241 cgcacactct gatcggcggc gaacagcacg ccgctgagac tgaacgcggc gagcgcggcg
     3301 agcgcggcga gcgggcggat catcggcaag gcgcctcagc cgaggctgcg attgaggatc
     3361 agcgccaggc gcacgccggc gcgcttgagc tgcagtcgca ccgcttcggc agcactctgc
     3421 atgtaggccg ctgacaggtg atagcggtgc gtgtcgtagg ccgcgggcag cagcccgaag
     3481 ctggtacgcc gggccatcgc aaacgactcc atggcccagt cggaggccga gccgcgttgc
     3541 cagctcgcaa gttcctgcgg gctgatctga tcgatcagca tcgatgcgac gttgcgcgga
     3601 tcgacgccta gcgcctgcac gaactccaca tcccaatagc gatgcagatt ctgcggggac
     3661 agcccggggg catccacctg ttcgagattg ccaccctggt cgtgctgatc cgcagcgtgc
     3721 agcggctgat gtagatcgcc taccaggtgc aacaggaact gcagcgcgat acgtcgctcc
     3781 ccggtggatg ttttggtatc ggatagctcc gcctggaact ggtcgatctt atccaccaca
     3841 caatcccctg ccgcccccgc cgaggccggc gtgcctgccg gcagcagcgg tcggccaaag
     3901 caggcgtgcg gcacgctcgg gtcgtccagc tcaatgtcca cgtagtgcca gcgacgcgtg
     3961 gcctcgtagc gcgcgcgcgt gctgtcacga tccgagtcgc ggtagcgatc cgcccatacc
     4021 gactcgcccg ccatcgatcg ctcagccacc aggcccgtgc tatccgcttc cagcaggagc
     4081 cgcacacgct cgcgcacggt gggctgcagg tagtgctcgg cgatcagggc gaccacggca
     4141 tgaccctcat cgccccaggc ctgcgcatcc tgcccgatac ccatcgttac gcacgcaatc
     4201 agcagttgca gcgcacgcga tggatggaaa atccgcatgg cgtcaagtat ttcatgcgag
     4261 ccggggtttg agcgaactcc cgcacgtcct atggtctaat gttccaatgc gtcggggaaa
     4321 gacagcgctt ctgcggaact ggccagcggc cctgttcgcg ctcgttttca tcgtcagcct
     4381 gtgcccgctc acttcccatg cccgcgacgg catcctgtcc cgaccacagg gcgtggatca
     4441 atggcggacc caccgcatca ttgtcaaatg gcgcagcgag ggcgtcgcgg cggtgcagat
     4501 gaatcgggtc gaagaccgcg cggcgcgcct gtcgcgcacg tccggcattc actttagccc
     4561 ggcacgcaat ctctacggcc gcacggatgt gatgctgctc gatcacatcc cggcgcatag
     4621 cgagatgcag gacatcctcg cgcggctgaa tgccgatccg gggatcgaat acgcggaacc
     4681 cgatgaggtg cgcttcctgc aggatttccc gatcaccacc cagatgccga acgactcgca
     4741 cttctacgcc agcaccgacc cgatgaacgc cggctccccg gacctggcga tcggcccctg
     4801 ggtcgggcag tggtacttgt tgccgagttc cagcaccact ccgtccgcga tctcggccac
     4861 caccgcctgg gccacggcca cgacacttgg caagtatgtg gtcgtcgcca tcatcgacag
     4921 cggcatcgcg caggaccacg aggacctcgc gcagaacatg ctgacgccgg gatacgattt
     4981 cgtgagttgc gatgagggca atttcgccag tacgacgacc accgcgctgg gtgcgacgca
     5041 gggcatcaat caatgcaccg ccagtggcag cgctgagacc tatttcttct cgaatgagaa
     5101 ttccaactgg tatgccgacg gcaccgatcc cggtgatttc atcgacaccg ccgatctggc
     5161 cttgcccgag ttcgcgaacc tcggctgcaa gcagacggtc gccagttcct ggcacggcac
     5221 caaggtggcc ggcgtgctgg gcgcggtggc gaacaatggc aagggcatcg ccggcgtcgc
     5281 cccggccgtc accatgatct ccgtgcgtgt gatcggcagc tgccgtggcg cactgctatc
     5341 ggatatcgcc agcgccatcc tgtgggcggc gggccagtcc gttcccatca gcactggaaa
     5401 tattagctcg agtcccaagg ccaacatcat caacctcagt ctgggtgcca acaccccctg
     5461 cgccgcgacc gagcaggatg ccatcaacgc ggcggtgaac gccggggtgc tggtggtcgc
     5521 ggcggcaggc aatgaaggcg gcgcgatcga tgcc
//
LOCUS       DATMRM010000041         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_331114, whole genome shotgun sequence.
ACCESSION   DATMRM010000041 DATMRM010000000
VERSION     DATMRM010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331114"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VNZ06_01125"
     CDS             <1..960
                     /locus_tag="VNZ06_01125"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HWW19379.1"
                     /translation="SVASLLSAADIACYAAKVSGRNRVHLYDSNEVSGRHREMYWVSR
                     VTRAVDEGRLELFYQPIAPTGCQAPALPDAYELLVRLRDDNGELVLPGEFVPAAERYN
                     VVSAIDRWVVQRAVQVLQKHSQGSDEPAFLFALNLSGMSLSDRSFLDFVLTLVEDPKI
                     ARGLCFEVTETAVITSMAQAVYFMHELRRRGCRFALDDFGSGLSSFHYLKTLPVDFIK
                     IDGQFIGNVTSDVVDRSMVEAISQVGRALGIATIAEKVESEAVFTELKRLGVQFAQGY
                     FIAHPAPIGNLIGASPSVAESHVTELPQESSAHCASRAVAYSP"
     gene            complement(984..>2167)
                     /gene="chrA"
                     /locus_tag="VNZ06_01130"
     CDS             complement(984..>2167)
                     /gene="chrA"
                     /locus_tag="VNZ06_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HWW19380.1"
                     /translation="FFRLGLRSFGGPIAHLGYFREEFVVRRRWIEESAFAELVGLCQF
                     LPGPTSSQVGFSLGLMRAGYRGALAAWLGFTLPSAALLVLFALGAPRLHSHWGVPLLH
                     GLRLVALPIVAQAVATMARALTPDLRRIGIAVLAAAVVLVAPGTLAQLSALLLGAALG
                     LILCRQILQPGALSSPFHGSRVVATFCLASFWLLLLACMLFPLLDARGSGSLFGAFYR
                     SGALVFGGGHVVLPLLRDALVKPGWVSDEAFLAGYGAAQAMPGPLFSLGAYLGFVATS
                     GPHGLAGAALGLTALFLPGILLLLAALPYWGQLQAAPALQSAFHGVQAAVVGVLAAAL
                     YDPIWKNSVHSFWDFVLALIALALLVLWRLPALLVVLVTALGAVAAAADPWALLQPLV
                     H"
BASE COUNT          428 a          683 c          721 g          335 t
ORIGIN      
        1 agcgttgcca gcctgttgag cgcggccgac atcgcctgct atgccgccaa ggtttccggc
       61 cgcaatcgtg tacacctgta cgactcgaat gaagtctcag gccgtcatcg cgagatgtat
      121 tgggtcagcc gcgtcacgcg cgcggtagac gaggggcggc tggagttgtt ttatcagccg
      181 atcgcgccga ccggttgcca ggcgccggcg ctgccggacg catacgaatt gctggtacgc
      241 ctgcgggacg ataatggcga gctggtgctg ccgggcgagt tcgtgccggc tgccgagcgc
      301 tacaacgtgg tctcggcgat cgatcgctgg gtggtgcagc gtgcggtaca ggtgctgcag
      361 aaacactcgc agggcagcga tgaaccggcg ttcctgttcg ctctgaacct gtcgggcatg
      421 tcgctcagtg accgcagttt tctcgatttc gtcctcacgc tggtcgagga tccaaagatc
      481 gcgcgcggtc tgtgtttcga ggtgacggag acggcggtca tcacgagcat ggcgcaggcc
      541 gtgtacttca tgcatgagtt gcgtcggcgt ggctgtcggt tcgcgctgga tgatttcggc
      601 agcggcttgt cttcgttcca ctatctgaag acgctgccgg tggatttcat caagattgac
      661 ggtcagttca tcggcaacgt caccagcgac gtcgtggacc gcagcatggt cgaggcgatc
      721 agccaggtcg gtcgcgcgct cggcattgcc acgattgcgg agaaggtcga gtcggaggcg
      781 gtatttaccg aactcaagcg cctgggcgtg cagtttgcgc agggctattt catcgcccac
      841 ccagcgccga tcggcaatct catcggcgcg agccccagcg tggcggagtc acacgtgacc
      901 gagttaccac aagagtcatc ggcgcattgc gcatcacggg ctgtggcata cagcccttag
      961 cttaaggcgc aggacgtgcg ccttcagtgc acaagaggct gcagcagcgc ccagggatcc
     1021 gcggccgccg cgacggcgcc cagcgcggtc acgaggacca ccagcagcgc cggcaagcgc
     1081 cagaggacca gcagcgcgag ggcgatcaat gccaggacga aatcccagaa gctgtgcacc
     1141 gagttcttcc agatcgggtc gtacagtgcg gcggccagca cgcccacgac ggcggcctgg
     1201 acgccgtgga aagccgattg cagggcaggg gcggcctgca gctgccccca gtaaggcagc
     1261 gcggcgagca gtagcaggat gcctggcagg aatagtgcgg tcaggcccag tgccgcgcct
     1321 gccagcccgt gtggtccact cgtggcgacg aaacccaggt acgctccgag gctgaacaac
     1381 ggacccggca ttgcctgggc ggctccgtac cctgcgagga acgcctcatc gctcacccag
     1441 ccgggcttca caagcgcatc gcgcagcagc ggcagtacaa catgcccgcc gccgaatacc
     1501 agcgcgccgg atcgatagaa cgcgccaaat agcgacccac tgccgcgtgc atccagcagc
     1561 ggaaacagca tgcacgccag cagtaacagc cagaagctcg ccaggcagaa ggtcgcgacg
     1621 acacgcgatc cgtgaaatgg gctcgacaat gcgccgggtt gcaatatctg tcggcacagg
     1681 atcaggccca gtgccgctcc gagcagcagc gcgctgagtt gagcgagcgt gccgggtgcg
     1741 acgagcacga ctgccgcggc gagcacggca atcccgatgc gcctcaagtc cggcgtgagc
     1801 gcacgtgcca tcgtggcgac ggcctgtgcg acgatcggca gcgcgaccag ccgcagtccg
     1861 tgcagcaacg ggactcccca gtggctgtgc agccgaggtg cgcccagggc gaacagcacc
     1921 agcagcgccg ccgagggcag cgtaaatccc aaccacgccg cgagggcgcc gcgatatccg
     1981 gcgcgcatca atccgagtga aaagccgacc tgactgctgg tcggaccggg cagaaactgg
     2041 cacagaccca ccagttcggc gaatgcggat tcttcgatcc agcgacggcg aacgacaaat
     2101 tcctcacgaa aatagccgag gtgcgcgatc ggtccgccaa agctgcgaag accgagccgg
     2161 aagaaca
//
LOCUS       DATMRM010000042         6104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1875240, whole genome shotgun sequence.
ACCESSION   DATMRM010000042 DATMRM010000000
VERSION     DATMRM010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875240"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="VNZ06_01135"
     CDS             complement(<1..1545)
                     /locus_tag="VNZ06_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWW19381.1"
                     /translation="MSGREAAVLSSELEFCLNQAFHQARTARHEFLTVEHLLLAILDA
                     PKVREVLEGCGADLDRLSADLKQHVENNTPRLSPSEEREVQPTLGFQRVLQRAVFHVQ
                     SSGKKEVGVVNVLVAIFSEKQSHAVFLLARGQVTRLDVVNFISHGLPRSGEAKSEKER
                     DDAGVEGERDSDSGSALEKYAVNLNAQAQEGRIDPLIGRKLEIERTIEILCRRRKNNP
                     LYVGEAGVGKTAIAEGLARLIVEGRVPEVLADCTIFALDMGALIAGTKYRGDFEKRLK
                     SVVTELKKQPGAILFIDEIHTVIGAGAASGGVMDASNLIKPVLANGEIRCIGSTTYQE
                     YRGIFEKDHALARRFQKIDVAEPSVAETVEILMGLKPKFEEHHGVEYSDDALRVAAEL
                     ADRHINERHLPDKAIDVVDEAGARLRLRPAGEREGRVEVSHIEDVVARMARIPAKNVS
                     ASDRDLLKNLERNLKLVIFGQNQAIETLAAAIKMARSGLGDQRKPVGSLLFAGPTGVG
                     KTEVTRQ"
     gene            complement(1553..1876)
                     /gene="clpS"
                     /locus_tag="VNZ06_01140"
     CDS             complement(1553..1876)
                     /gene="clpS"
                     /locus_tag="VNZ06_01140"
                     /inference="COORDINATES: protein motif:HMM:NF000672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HWW19382.1"
                     /translation="MTEHRNSGRPDHGVVVEEAPPETAQPPLYRVILLNDDYTPMEFV
                     VDVLEKFFAMDRPTATRVMLEVHTRGKGVCGVFTYEIAETKVSQVTIYSREHQHPLLC
                     TLEEA"
     gene            2117..3163
                     /gene="gspC"
                     /locus_tag="VNZ06_01145"
     CDS             2117..3163
                     /gene="gspC"
                     /locus_tag="VNZ06_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HWW19383.1"
                     /translation="MDELKSGQALSRLLAAHGAQIAAIVLLLLLGLDSALIVTRAFDK
                     PLAAVPPPSGLPRTRPTINPTLQLALIVNAHLFGSAAIASNGDAPQTTMPLILAGVIA
                     DKDPNKGQAIIGENATAAKLYAVGGSIPGGARLHAVYADRVLLERNGALEALSLPRNE
                     SPAGRGINTAPPLSLGTRLGAAASANPTLLAGLVRVQPVFTQGRLSGYRIFPGGGRGN
                     AAFTQLGLKPGDLIEAVNGTALDDASRAMEVLQTLGSSASATVTVSRNGQSQEVNLNL
                     ANLNIEPDLGGAATPPISEAGQSIPPVGPPASPNPRPRRGDAANMGIVPGNPTNTDAT
                     IPPPGTIPPGERER"
     gene            3210..5546
                     /gene="gspD"
                     /locus_tag="VNZ06_01150"
     CDS             3210..5546
                     /gene="gspD"
                     /locus_tag="VNZ06_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HWW19384.1"
                     /translation="MSDRVLTVRPHARRNRIALAVGLLTVSACALSQQTASDAPGTRA
                     TGCSAPCVTPNFKDADITQIIEAVRNVTGKTFIIDPRVRAQVTMLSSTPMTPSQFYEA
                     FLSILQVHAFMAVPGPGNVVKILPDTNMRQYPSLDLPDRVSGTSDEVVTQVLAVKNVS
                     AAQLVPVLRPLAAQNAQLSAVTGANILIISDHSSNVNRIMRIIERIDQVGNPDYDVIT
                     LHSATAADTARVLNSLLAQSGEAAGMIKIVADDRSNSILLSGDETTRLRVKTLIAHLD
                     TPIDAGSETQVRYLRFSDAEDLATRLKEQLSGTSSGGSSSSSLGSNVRNVPYQPTPNS
                     QGNTGPLPNPNASSASSGPATLTLKGETATIWADKATNALVITAGARGMRALNAVIDK
                     LDIRRPQVYVEAIIAEVTVDKTSDLGVNWALDGTNSSVGVGGFVSPIGGTSIVDLANA
                     ALGATATSSSINGTTTTSPGVSAAGLSNQTLNGTTFGVGRLRASGVNFAAVVRALQSD
                     SRTNILGTPSVVTRDNQEAKMEVAQEVPFLTGQYSTTNGTGSAFQTIQREDIGTILTV
                     TPTINEGNTVLLKLQVESSSLAASASGAVDLITNKNSITTSVLVKDGGTLVLGGLIQD
                     SVTNSESQVPILGSIPILGELFRTRNTSKTKTNFLIFLQPHILRSDEQASEETDAKFN
                     YIRDQQTILNRDKKIIPFQPFAPADPLPSISEGRTKGGILSPDANTAPPPSSTASPAP
                     SAPGTPLAPDSSQPPAAPPSQTPAVTSPDNSQSAGPAK"
     gene            5543..>6104
                     /locus_tag="VNZ06_01155"
     CDS             5543..>6104
                     /locus_tag="VNZ06_01155"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWW19385.1"
                     /translation="MSEPAERVDSGDFRLPFAFARRHGVVVLGIESGVADCAYRAPAS
                     PLALAETRRYLQLPVKLTAVDSDQFDLLLRKTYEGGNGEAMQQIGLEDTTDLAHLAQE
                     LPEQADLLESDDDAPIIRLINAVLTQAIRENASDIHIEPFENRLVVRFRVDGILREVL
                     QSKRGVAPLVVSRIKVMSKLDIAEKRL"
BASE COUNT         1170 a         2011 c         1824 g         1099 t
ORIGIN      
        1 ctgcctggtc acttcggtct tgccgacgcc ggtcggaccg gcaaacagca ggctccccac
       61 gggtttgcgc tgatcgccca aacctgagcg cgccatcttg atggcggccg ccagggtttc
      121 gatcgcctgg ttctgaccga agatcacgag cttcagattg cgctcgagat tcttgagcag
      181 atcacggtcc gaagccgaga cgttcttggc cgggatgcgg gccatgcggg ccaccacgtc
      241 ctcgatatgc gagacctcaa cccggccctc gcgctcaccg gcgggcctga ggcgcagtct
      301 cgcaccggct tcatccacca catcgatcgc cttgtcgggc aggtggcgct cattgatatg
      361 acggtcggcg agttccgctg cgacgcgcag cgcatcatcc gagtactcca cgccgtggtg
      421 ctcttcgaac ttcggcttca gacccatcag aatctcgacc gtctcggcca cggaaggctc
      481 ggccacgtcg atcttctgga agcgacgcgc cagcgcgtga tccttctcga aaatgccacg
      541 gtattcctgg taggtggtgc tgccgatgca gcggatttcg ccgttggcca gcaccggctt
      601 gatgagattg gaggcatcca tgacgccgcc ggaggccgcg cccgcgccga tgaccgtgtg
      661 aatctcatcg ataaagagga tcgcgccagg ctgcttcttg agctcggtta cgaccgactt
      721 caggcgcttc tcgaagtcgc cgcgatactt cgtgcccgcg atcagtgcac ccatgtcgag
      781 cgcgaaaatc gtgcagtcgg ccagcacctc ggggacccgc ccttcgacga tcaggcgagc
      841 cagcccttcg gcgatcgcgg ttttgccgac gccggcctca ccgacataca gcggattgtt
      901 cttgcggcgg cggcacagaa tctcgatcgt gcgctcgatc tcgagcttgc ggccgatcag
      961 tggatcgatc cgcccttcct gcgcctgcgc attcaggttc accgcatact tttccagcgc
     1021 gctgccggaa tcgctatcgc gttcgccctc gactccggcg tcatcccgtt ccttctcgga
     1081 tttggcttca cccgagcgcg gcagaccgtg ggaaatgaaa ttcaccacat ccagtcgtgt
     1141 cacctgcccg cgggccagca ggaatacagc gtgactttgc ttctcgctga agatcgccac
     1201 cagcacattg acgacgccga cttccttctt gccgctggat tgcacatgga acacggcccg
     1261 ttgcagcacc cgctggaatc cgagggtcgg ctgcacttcg cgctcctcac tgggtgagag
     1321 gcgcggggta ttgttctcga cgtgctgttt gaggtcggcg ctcaggcggt caaggtcggc
     1381 gccgcagccc tcgagtactt cccggacctt gggggcgtcg agaatggcca gtagcaggtg
     1441 ctcgacggtg aggaattcgt ggcgcgcggt tcgcgcctgg tgaaaggcct ggttgaggca
     1501 aaactcaagc tcgctcgaca acactgctgc ttctctcccg ctcacgcgtc cgtcaggctt
     1561 cctcgagcgt acacagcaat ggatgctggt gctcacgcga ataaatggtt acctgcgaca
     1621 ctttggtctc tgcgatttcg tatgtaaaga ctccacaaac ccctttgcca cgagtatgca
     1681 cctccagcat cactcgtgtg gccgtcggcc ggtccatagc gaaaaatttt tccagcacat
     1741 cgactacgaa ttccatggga gtgtagtcat cattgagcag tatgacgcga taaagagggg
     1801 gctgcgcggt ttccggcggt gcctcctcga cgacgacgcc atgatcggga cgaccggaat
     1861 tccggtgttc cgtcacttga attctgccct gtcacacaat cgcagtgcca gcaaagcgca
     1921 tcggtcactg aagtctaatc cactgcgtct catcggtgaa cgcctaggaa ctccgtcaca
     1981 ttgtcggcaa caagcgacgc acggaactaa aagaacttag ccgaaacaaa cacttgagag
     2041 cgtcaacggc gaaagcctat catggcatat cttagtgctc ctctggagct aaaagttggc
     2101 tagcgcagcg gcttggatgg acgagctcaa atcggggcaa gcgttgtcgc ggctgctggc
     2161 cgcacatggc gcgcagattg ccgcgatcgt gctgctgctg ctgctgggac ttgacagtgc
     2221 gctgatcgtg acgcgcgcgt tcgacaaacc gctcgctgca gtgccgccgc cctcgggcct
     2281 gccaaggacg cgcccgacca tcaatcctac gctgcagctc gcactgatcg tgaacgccca
     2341 cctgttcggt tccgcggcca tcgccagcaa tggcgatgca ccgcaaacca ccatgccgct
     2401 gattctggcg ggggtgatcg cggacaagga cccgaacaag gggcaagcca tcatcggtga
     2461 gaatgccacc gcggcaaagc tttacgcggt cgggggctcc atcccgggtg gcgcgcgcct
     2521 gcacgccgtc tatgcggatc gcgtcctgct cgagcgcaac ggtgcacttg aagccttgag
     2581 tctgccgcgc aatgagtcac ccgctggtcg tggcatcaac accgcacccc cgctctctct
     2641 gggcacacgc cttggagcgg cagccagtgc caatccgacc ctgctcgcgg gcttggtacg
     2701 cgttcagccg gtctttacgc agggcaggct ctcgggctac cgaatctttc cgggaggcgg
     2761 ccgcggcaat gccgcgttca cgcagctcgg actgaaaccg ggggatctga tcgaggcggt
     2821 caacggcaca gcgctcgatg atgccagccg ggcgatggaa gttctgcaga ccctgggttc
     2881 ttccgcctca gcgaccgtca ccgtctcgcg caacgggcag tcgcaggagg tcaacctcaa
     2941 tctggcgaac ctcaacatcg agccggatct aggcggtgcc gccacgccgc cgatctccga
     3001 ggcgggacaa agcataccgc ctgtgggtcc gccggcatcc cccaacccgc gcccgcggcg
     3061 tggggatgcg gcgaatatgg gaattgtgcc cggtaatcca acaaataccg atgccacaat
     3121 tcctcctccg ggcacaattc ctccgggcga gcgggaacga tagtcgccca gagcagtcag
     3181 cgatagacga cagacaggaa cgtttattaa tgtccgatag agtattgacc gttcggccgc
     3241 atgcgcggcg gaatcgcatt gcgctcgccg tcggcttgct caccgtcagc gcctgcgcac
     3301 tctctcagca gaccgccagc gacgcgcccg ggacgcgcgc caccggctgc tccgcgcctt
     3361 gcgtgacgcc caacttcaag gacgccgata tcacgcagat catcgaggcg gtccgcaacg
     3421 tcaccggcaa gaccttcatc attgacccgc gcgtgcgagc gcaggtgacg atgctgtcgt
     3481 ccacgccgat gaccccgtcg caattctacg aagcgttcct atcgatcctg caggtgcacg
     3541 ccttcatggc cgtgccaggt ccgggcaacg tcgtcaagat cctgcccgac accaacatgc
     3601 gccagtatcc gagcctggat ctgcccgatc gcgtcagcgg cacgtcggat gaggtcgtga
     3661 cgcaggtgct ggcggtcaag aatgtcagcg ccgcacagct ggtgccggtg ctgcgaccgc
     3721 tggctgcgca aaatgcgcag ctgtctgccg tcaccggagc gaacatcctc atcatttcgg
     3781 atcactccag caacgtgaac cgcatcatgc gcatcatcga gcgcatcgat caggtcggca
     3841 atcccgacta cgacgtgatc acgctgcaca gtgccacggc tgccgacacc gcgcgcgtgc
     3901 tcaactcgct gctcgcgcaa agcggtgagg cagccggcat gatcaagatc gtcgccgacg
     3961 atcgctccaa cagcattttg ctcagcggcg atgagaccac gcgcctgcgc gtgaagaccc
     4021 tgatcgctca tctggacacg ccgatcgatg ccggcagcga gactcaggtg cgttacctgc
     4081 gcttctccga tgcggaagat ctggccaccc gtctgaagga gcagctctca ggcaccagca
     4141 gcggtggcag ttcaagctcg agcctgggca gcaatgtacg caatgtccct taccagccga
     4201 cgcccaattc ccagggcaac accggcccct tgcccaatcc gaacgccagc agtgcctcgt
     4261 cgggacctgc gacgctcacg ctgaagggtg agaccgctac catctgggcc gacaaggcca
     4321 ccaatgcgct ggtcatcacc gcgggcgcgc gcggcatgcg cgccctgaat gcggtgatcg
     4381 acaaactcga tatccgtcgt ccacaggtgt acgtcgaggc catcatcgcg gaagtcacgg
     4441 tggacaagac ctccgatctg ggcgtgaact gggcattgga tgggaccaac tccagcgtcg
     4501 gcgtcggcgg cttcgtctcc ccgattggcg gcacgtcgat cgtggacctg gccaatgcgg
     4561 cactgggcgc gaccgcgacc agctcgtcga tcaacggcac gaccaccacc agcccgggcg
     4621 tgagcgcggc gggattgtcc aatcagacat tgaacggcac gaccttcggc gtcggtcgcc
     4681 tgagagccag cggcgtgaac tttgcggccg tggtgcgcgc gctgcagtcc gactcgcgta
     4741 ccaacattct gggcacaccc tcggtcgtga cgcgtgacaa ccaggaagcg aagatggaag
     4801 tggcacagga agtaccgttc ctgaccggtc agtattccac gaccaacggg accggcagcg
     4861 cctttcagac cattcagcgc gaggacatcg gtacgattct gaccgtgacg ccgacgatca
     4921 acgaaggcaa cacggtactg ttgaagctgc aggttgagag ttccagcctg gcggcgagcg
     4981 cttcgggcgc ggtggacctc atcaccaaca agaattcgat cacgaccagc gtgctggtca
     5041 aggacggcgg caccctggtg ctcggcggcc tgatccagga cagcgtcacc aacagcgaat
     5101 cgcaggtgcc tatcctgggc agcatcccga ttctcggcga gctgttccgt acccggaaca
     5161 cctccaagac caagaccaac ttcctgatct tcctgcagcc gcacatcctg cgcagcgatg
     5221 agcaggcttc ggaagagacc gacgcgaagt tcaactacat tcgcgatcag cagaccatcc
     5281 tcaatcgcga caagaagatc attccgttcc agccgttcgc gccagccgat ccgttgccgt
     5341 cgatatcgga aggccgtacc aagggcggca tcctcagtcc cgacgccaac accgcgccgc
     5401 cgccgagttc cactgcgtca ccggcgccga gtgcgcctgg cacaccgctt gcgcccgact
     5461 catcgcagcc gccggccgcc ccacccagcc agaccccggc ggtgacctct ccggacaact
     5521 cgcaatcggc ggggccggca aaatgagtga accggctgaa cgcgttgaca gcggcgactt
     5581 tcgcctgccg ttcgcgttcg cacggcgtca cggtgtggta gtgctgggca ttgaatccgg
     5641 tgtggccgat tgcgcctatc gcgcgcccgc ctctccgctg gcactcgccg aaacgcgccg
     5701 ctatctgcag ctgccggtca aactcacggc ggtcgacagc gaccagttcg acctgctgct
     5761 gcgcaagacc tatgaaggtg gcaacggcga ggcgatgcag cagatcggtc tggaggacac
     5821 gaccgacctg gcgcatctgg cgcaggaact gccggaacag gccgacctgc tggaaagcga
     5881 tgacgatgcg ccgatcatcc gactcatcaa tgcggtgctg acgcaggcga tccgcgagaa
     5941 cgcctcggat attcacatcg agccgttcga gaatcgactg gtggtgcgtt ttcgcgtcga
     6001 cggcatcctg cgcgaagtgc tgcagtccaa gcgtggggtc gcgccactgg tggtctcgcg
     6061 catcaaggtc atgtcaaagc tcgacatcgc cgagaagcgc ttgc
//
LOCUS       DATMRM010000043         8882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4360648, whole genome shotgun sequence.
ACCESSION   DATMRM010000043 DATMRM010000000
VERSION     DATMRM010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8882
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4360648"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VNZ06_01160"
     CDS             complement(<1..583)
                     /locus_tag="VNZ06_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW19386.1"
                     /translation="MISVLFVDDETHVLDGLRRSMHSMRAEWRMRFCTSGTDALQQLS
                     AEPADVVIADMRMPTMDGSQLLAEVKRHYPHAVRFILSGQAERESVIRATRNAHRYLS
                     KPCDAASLRTAIARALSLKALLANDALDEIVGRVDALPTPPTMYVRLRECLRGADTSI
                     EDVVDLIQQDVALTAKVLKLANSGFFGRREPVES"
     gene            complement(715..2214)
                     /locus_tag="VNZ06_01165"
     CDS             complement(715..2214)
                     /locus_tag="VNZ06_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19387.1"
                     /translation="MGARRFDSRYYLVSSLALIGTCVSHGALAADDSVQAFIDPSGIT
                     ATVDINGRVNTNSAFFQSLGTNGRSCSTCHVASQAMSISPSDVRERFARSAGSDPLFA
                     PVDGANCPTGRSGVAADHSLLLQHGLIRIAIAMPANAQFTISVVHDPYGCAILPNPAG
                     GPFVSVYRRPLPSSNLGFLSTVMFDGRETAQPLNSKPTFINNLTQDLTNQALHATQGH
                     AQASTNPSSAQLADIVNFELGLFSAQAFDARAGWLHAQGADGGPAFLAGLQPEYYPGQ
                     NDVLGADPDGLPFNNSAMTLFNAWSNIQNNDGDYFEWIRDEARRAIAAGEVVFNTATA
                     HISTVRGLNDNAALGKPAVFNGNCTTCHDTPDVGNHSLPLPLDIGTSHSTRSGMENDP
                     NIIAGLSQMSMPDLPVYLISGCPNPFNPGEPESFYTSDPAKALITGQCSDFNRGKGPV
                     LRGLAARAPYFHNGSAANLTELVNFYDQRFQMNLTAQQKSDLIAFLNSL"
     gene            2350..2871
                     /locus_tag="VNZ06_01170"
     CDS             2350..2871
                     /locus_tag="VNZ06_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HWW19388.1"
                     /translation="MDRECSAASAQNQQLFAALYSDLRKLAESQLRRSQVGQISPTTL
                     LHEAYLGMSGRENRFPDRERFMGYAARVMRGLIIDFVRERRALKRGAGYQLTQLPTEL
                     PVAVEPDLTRLSEALDVLATRDARLAEIVDLRYFCGFSLEDVAALRGTSVRTVQRDWE
                     KARVILFEGLQSD"
     gene            2938..5619
                     /locus_tag="VNZ06_01175"
     CDS             2938..5619
                     /locus_tag="VNZ06_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWW19389.1"
                     /translation="MANLSDEQWRSFSEHLDQALQLPEADLPGWLAALASSQPQVAAE
                     VQRKLQARHRDGFASFLSEQLIVNPQQAGEPLVGRQVGHYTIESEIGRGGMGSVWRAH
                     RTDGRFEGTVAIKFVHTYWLGRRAAEERFLAEGRLLGRLSHPHIARLIDAGVFDDSMP
                     YLVLEYVEGKPIDEYCQRRHLDTGSSVALFRDVLAAVAHAHSHLIIHRDLKPSNILVT
                     HDGVVKLLDFGVAKLLGEEASATRTAAPALTPQYAAPEQLQGEAVTTATDVYALGLVL
                     YRLLTGQHCVQTDGRSTADIYHAVIEVPPSRASRMADIPPATRRALEGDLDNILGKSL
                     RKDPDERYGSIGEFAEDLRRYLAHEPVRARPDTVPYRLRKFVRRNRGSVFGGMLVALG
                     LIGTSVFALMQMREARAQRDLALDEARRASAEADLTQYILDDRLSKLTPQAEHERLDR
                     AREFIGSRFQAQPTLTAQLLIDLSGRYIDLGDFTTAAEVIVEADGIGHRSDDATVLGQ
                     IACLRTEDLAIARDYVSARGQLTAGIAQMRRLRPVPWQVEAECATAEAFVLQADGNFD
                     VPATHLRQTLAHLQADGLHGSGRYFSTANDLARAYLLAGRYRQALDISVANVELAQQL
                     GRADTYSAAIYATYVCQALRNGGQPRRAVEYLDAHTARMRASGGDESLSAATIGCRGV
                     ALLDQGQVQTAQPAILMAMQKAERGAATYVASQYHAAAVAAAIARGDLAGAEGLWEPL
                     AKDESERLAAKRRGVEVVRLLLVHAQLDLARGHPDDALASLARGESILQARHQPSNPE
                     AWDIESLRSRALLSKADYAQAMRHAQNALDLARASAIDPQSSHYIGEALLWRARAEAA
                     QGDKHSAASAHEALPHLINNLDLGNPLIAEAQKLASP"
     gene            complement(5713..7659)
                     /locus_tag="VNZ06_01180"
     CDS             complement(5713..7659)
                     /locus_tag="VNZ06_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19390.1"
                     /translation="MQRVRKSSWPAGAAVLCLAVAPFAHAQHASDDPLAIADDAFGLT
                     LGLESIGLYGPGGVRGFNPQVAGNVRINGLYFDQQGGLSGRVIEGSTIRIGVSEVGYP
                     FPAPTGIVDYDLRHVGDGTATATVVASLGPFQNRGLSLDGDIPVLGRNLQLPIGASSQ
                     IVTNSPYAGDKGYTAKYLDFGASPQWTANDRITLRAFIDWQQYTSARTMPLVFPAGAF
                     IPPTVDGYIGQNWALGASWSENYGALITAQLTPHWYLSAGVFRSIFDQPESYSDLYLN
                     SNPVGLADHRVVGYPEQSIASTSGEVRLTGHYLTGPLRHELILDARGRDTLATYGGSD
                     AVDVGPALVAADAQVRRPAFIYGARTNDRAQLWSMGTAYRLQWQGRADLAVGVQRESY
                     DKIVVTPGLPIAHLEDRPLRAYATAAVTLAKPVTLYGSYTQGLEESGTAPGNAENRGM
                     ILPAARTQQQDAGFRFELTPKLKLITGAFEIEKPYFNLDTRNTDRQLGTQRARGVEVS
                     LSGEVSEQLKLSFGVLAGKVAIIGSDLGAEGVGPVAVGQPRTTYLFNADYHLKGWPAL
                     SFDGIYYRFGAAPASVDNTLYNPEQTILSLGSRYRFKVLGHPATLRLFWQNFNKEDFW
                     NLGLSPGFLQFPGQSILGYVTADF"
     gene            complement(7669..>8882)
                     /locus_tag="VNZ06_01185"
     CDS             complement(7669..>8882)
                     /locus_tag="VNZ06_01185"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HWW19391.1"
                     /translation="EGPATMKPLLGEIGSGTVAGIANHLWQSTLVALAAALTAWLLLR
                     RNEAGVRHGLWFAASIKFLLPFSLLISAGRSISSLLEVGHREAWWQNAVIRVSEPFSL
                     SSDPHASKPFALMATLLLAVWATGLVVVLTVWVLRWRSIARIARAGIVVSDAREVRVL
                     RQVERQMGIRRPITLLLCSASLEPGIFGIWRPVLLWPQGISAHLTRSHLQAVIAHEVC
                     HVRRRDNLAAAIHMVVEAVFWFHPLVWWLGTRLMRERERACDQKVLELGSERAVYAES
                     ILKTCQYCVGSPLPVLSGVTGAELKQRVVDIMTGEAARRLGFSAKLALSSCAVLALVA
                     PTLFGVWRVYVCDGEAYAPVSRPAMCDAIDGRKKPFAGDMRTCRVLADELLSVPQLPD
                     AMRQIQENGGT"
BASE COUNT         1603 a         2774 c         2921 g         1584 t
ORIGIN      
        1 tgctttccac cggttcacgc cgtccaaaga agcctgagtt agccaactta agaaccttgg
       61 ctgtcagtgc gacatcctgc tggatcagat ccacgacgtc ctcgatggag gtgtccgccc
      121 cgcgcaggca ctcacgcaat cgcacgtaca tcgtcggcgg agtaggcaac gcatccacgc
      181 ggccaacgat ctcatcgagc gcatcatttg ccaggagcgc cttgaggctc agcgcgcgcg
      241 cgatcgccgt cctcagcgag gcagcatcac aaggtttgga cagatagcgg tgcgcgttgc
      301 gcgtggcgcg gatgacggac tcgcgttcgg cctgccctga aaggatgaag cgaaccgcgt
      361 gcggatagtg gcgcttgacc tctgcgagaa gctgtgagcc atccatggtc ggcatgcgca
      421 tgtcggcgat cacaacatcc gctggctccg cggatagctg ctgcagcgcg tcggtcccgc
      481 tcgtgcaaaa tcgcatccgc cactcggcgc gcatgctatg catgctgcgc cgaagtccat
      541 cgagcacatg ggtctcgtcg tcgacaaaca gaacgctgat cacttcggcc ctgtccgagt
      601 atgacttaat tgtaaagcgc tgttctacgc ctgcaccttc gccatgagat gcgatggtcg
      661 tcacaatcag ctacaccggc ccattggatt gtgaccggat gctcggtccg aaacctagag
      721 cgagttcagg aatgcgatca ggtcggattt ctgctgcgcc gtcagattca tctggaagcg
      781 ctggtcgtag aagttcacca gctcggtcag gttggcggcc gagccgttgt ggaagtacgg
      841 tgcgcgcgcg gcgagacccc gaaggacggg tcccttgccg cgattgaagt cgctgcactg
      901 cccggtgatc agcgccttcg cggggtcgga ggtatagaag gattcaggtt cgcccggatt
      961 gaaaggattg gggcagccgc tgatcagata caccggcagg tccggcatcg acatctgcga
     1021 caggcccgca atgatgttgg gatcattctc cataccggac cgggtgctgt gggacgtgcc
     1081 gatgtccagt ggcagcggca gggagtgatt gccgacatcc ggggtgtcat gacaggtggt
     1141 gcagttgccg ttgaacaccg ccggtttgcc cagggccgca ttgtcgttga ggccgcgcac
     1201 ggtggagatg tgtgcggtcg cggtgttgaa cacgacctct ccggccgcga tggcgcggcg
     1261 cgcttcatcg cggatccact cgaagtagtc cccgtcgttg ttctggatgt tggaccaggc
     1321 gttgaacagg gtcatcgccg agttgttgaa cggcagcccg tcgggatctg cgccgagcac
     1381 gtcgttctgg cccggatagt attcgggttg caagcctgcg agaaatgccg gtccgccgtc
     1441 cgcgccttga gcgtgcaacc atcccgcgcg ggcatcgaat gcctgagcgg agaacagacc
     1501 cagttcgaag ttgacgatat cggcgagctg cgcactgctg ggattggtgc tcgcctgagc
     1561 atgaccctgg gtggcgtgca gcgcctgatt ggtgaggtcc tgcgtcaggt tgttgatgaa
     1621 cgtcggtttg ctgttgagcg gctgcgccgt ctcgcgcccg tcaaacatga cagtgctgag
     1681 aaacccgagg ttcgaagagg gcagcggccg ccgatacacc gacacgaaag ggccgcccgc
     1741 ggggttggga aggatcgcgc agccgtacgg gtcatgaacc acggagatcg tgaactgcgc
     1801 gttcgcgggc atcgcaatcg cgatgcggat caggccatgc tgtagcagca ggctgtgatc
     1861 ggccgcaaca ccgctccggc cggtcgggca gttggcgccg tccaccggtg caaacagggg
     1921 atcgctaccc gcactgcgcg cgaatcgctc ccgaacatcg ctggggctga tactcattgc
     1981 ctgactggcc acatggcagg tcgagcagct gcggccgttg gtgccgagac tttgaaagaa
     2041 tgcgctgttg gtgttcacgc gcccgttgat atcgaccgtg gcggtgatgc cgctcggatc
     2101 gatgaaggcc tgtacactat cgtcggcggc gagggcgccg tgtgaaacac atgtcccgat
     2161 cagagcaagc gatgagacca ggtaatacct actgtcaaat cgacgtgcgc ccatcttgga
     2221 tcccctcccg cccacattgt gcgggctacc gtccaggtac agcgcaatcg gcacggcaaa
     2281 cgtgacattg cttgctagca gctgagcgtt tgggcatcct caaagctcgc gcagcccggg
     2341 attctcagga tggacaggga gtgttcagcg gcgtccgccc agaaccagca gctgtttgca
     2401 gcgctgtaca gcgatctacg caagcttgct gagagtcagc tgcgacgcag tcaggtggga
     2461 cagatcagcc cgaccacact gctgcacgag gcctatctcg gcatgtccgg gcgcgagaac
     2521 cggttccctg atcgcgagcg cttcatgggc tacgccgcgc gcgtgatgcg cggactgatc
     2581 attgatttcg tccgggagcg acgcgcactc aaacgcggtg ccggctatca actcacgcaa
     2641 ctgccaacgg agctgccggt cgcggtggag ccggatctga cgcgcctgtc ggaagcgctg
     2701 gatgtgctgg caacccgtga cgcgcgtctg gctgagatcg tggatctgcg ctacttctgc
     2761 ggcttctcgc tcgaggatgt tgcggcgttg cgtggcacct cggtccgcac ggtgcagcgg
     2821 gactgggaga aggcgcgcgt gattctgttc gagggcctgc aatcggattg atttcagtcg
     2881 tgccgagtcc gatggcgcga gcgatacgag aattacgatt tatgcaccgg gggaactatg
     2941 gcaaacctct cggacgagca atggcgctcc ttcagcgaac atctggatca ggcactacag
     3001 ctgcccgaag ccgatcttcc cggctggctc gccgctctgg catcctcgca gcctcaagtc
     3061 gcggccgaag ttcagcgcaa actgcaagca cgccatcgcg atggatttgc ctcgtttctg
     3121 tccgagcagc tcattgtcaa tccgcaacag gcaggcgagc cgctcgtcgg acggcaggtg
     3181 ggtcactaca cgatcgaatc ggagattggt cgcggcggaa tgggcagtgt ctggcgcgcg
     3241 caccgtactg acgggcgatt tgaaggcacg gtcgcgatca agttcgtgca tacctattgg
     3301 ctcggtcgtc gcgcggctga ggagcgcttc ctggccgagg gccggctact cggccggctt
     3361 agccatcctc acattgcgcg cctcatcgat gcgggcgttt tcgacgactc catgccgtac
     3421 cttgtgctcg agtacgtcga gggcaagccg atcgatgagt actgtcaacg ccgacacctc
     3481 gacaccggtt catccgtagc gctcttccgt gacgtgctcg ccgcggtcgc acatgcgcac
     3541 agtcatctga tcattcatcg cgatctgaag ccctccaata ttctggtcac acacgatggc
     3601 gtggtgaagc tgcttgattt cggggtcgcc aaattgctgg gcgaagaggc cagcgcgacc
     3661 aggacggcgg ctcccgcact cacgcctcag tacgccgcgc ccgagcagtt gcagggtgag
     3721 gcggtgacga ccgcgaccga tgtgtatgcg ctgggtctgg tgctctatcg gttattgacc
     3781 ggacagcatt gcgttcagac cgacggtcgt tcgacggctg atatctatca cgcggtgatc
     3841 gaagtgccgc catcgcgcgc ctcgcggatg gcggacatcc cgcccgccac gcgccgcgcg
     3901 ctcgagggcg atctggataa catcctcggc aaatcactca ggaaggatcc ggatgagcgc
     3961 tatggctcga tcggggagtt tgccgaggat ctgcggcgct acctggcaca cgagccggtg
     4021 cgcgcccgac cggacaccgt tccctatcgc ctgaggaagt tcgtgcgtcg caatcgcggc
     4081 agcgtattcg gcggcatgtt ggtcgcattg ggcctgatcg gcaccagcgt tttcgcactg
     4141 atgcagatgc gcgaggcacg cgctcagcgc gatctggccc tggatgaagc gcggcgtgcg
     4201 agcgccgagg cggatctgac gcaatacatc ctggatgatc gcctgagtaa actgacgccg
     4261 caggccgaac acgagcggct ggatcgcgcg cgtgaattca tcgggtcgcg ctttcaggcg
     4321 cagccgacct tgactgcaca gctgctgatc gatctgtccg gtcgctacat cgacctcggg
     4381 gacttcacca ccgccgcgga ggtgattgtc gaggcggatg gcatcgggca tcggtccgat
     4441 gatgccaccg tgctcggtca gattgcctgc ctgcgcaccg aggatctggc gatcgcgcgt
     4501 gactatgttt cggcacgcgg ccagctcacc gccggtatcg cgcagatgcg gcgcctgcgc
     4561 ccggtgccgt ggcaggtgga ggcggagtgt gcgaccgccg aggctttcgt gttgcaggcc
     4621 gatggcaact tcgacgtgcc ggcgacgcac ctgcgccaga cactggctca cctgcaggcg
     4681 gacggcctgc atgggtcagg gcgctatttc tcgaccgcga acgatctggc gcgcgcgtat
     4741 ctgctcgcgg gacgctatcg ccaggccctt gatatcagtg tcgccaacgt tgagctggcg
     4801 cagcaactgg ggcgagccga cacctacagc gccgcgatct acgcgaccta cgtctgccag
     4861 gcgttgcgaa acggcggaca gccgcgccgt gccgtcgaat acctggatgc gcacacggcg
     4921 cgcatgcgag cctcgggagg cgatgagagc ttgtcggcgg cgacgatcgg ttgccgcgga
     4981 gtggccctgc tggatcaggg gcaggtgcag acggcgcagc cagccatcct catggcaatg
     5041 cagaaggccg agcgaggcgc ggcgacttac gtcgcctcgc agtatcacgc ggcagcggtg
     5101 gctgcggcga tcgcgcgcgg cgatcttgcc ggtgcggaag ggctgtggga accgctggcg
     5161 aaggacgagt cagaacgact ggcggcaaag cggcgcggcg tcgaggtcgt gcggctgctg
     5221 ctcgtgcatg cgcaactgga tctggcgcga ggccatccgg acgacgcgct ggcttcactg
     5281 gcacgcggcg agagcattct tcaggcgcgt caccagccga gcaatcccga agcgtgggac
     5341 atcgagtcgc tgcgaagccg cgcgctgctg tccaaggccg attatgcgca agcaatgcga
     5401 cacgcgcaga acgccttgga tctggcacgg gcgagtgcga tcgacccgca gtcctcacac
     5461 tacatcggcg aggcactgct atggcgcgcg cgggccgaag ctgcgcaggg ggacaagcac
     5521 tcggctgcca gcgctcacga ggcattgccg cacctcatca acaacctaga cctaggcaac
     5581 ccactgattg ccgaagcgca gaagctggcc tcgccctaga gaatgggctt catgcgcggc
     5641 cggagcgtct cgagcgccag gcgcagtccc cgcgagacgc gtcgcgcccg gctgcatggg
     5701 caagcgcgca cgtcagaaat ccgcggtcac gtatccaagg atcgattgtc cgggaaactg
     5761 caagaagccc gggctcagac ccagattcca gaaatcctct ttgttgaagt tctgccagaa
     5821 cagccgcagc gttgccgggt ggccaagtac cttgaagcga tagcggctgc cgagactgag
     5881 gatcgtctgc tcgggattgt agagcgtgtt gtccacgctc gccggcgcgg caccgaagcg
     5941 gtagtagatg ccgtcgaacg acagcgcggg ccagcccttg aggtgataat ccgcgttgaa
     6001 caggtacgtg gtgcgcggct ggccgacggc caccgggccc acgccctcgg cgcccagatc
     6061 cgagccgatg atggccactt tgccggccag cacgccgaag ctgagcttga gttgctcgct
     6121 cacctcaccg gacagtgata cttcaacgcc gcgcgcgcgc tgcgttccga gctgacggtc
     6181 ggtgttgcgg gtatcgaggt tgaagtaggg cttttcgatt tcgaacgccc cggtgatcag
     6241 tttcagtttc ggcgtcagct cgaatcgaaa gccggcgtct tgctgctgag tgcgcgcggc
     6301 cggcagaatc atgccgcgat tctctgcgtt tccgggagcg gtgcctgatt cttccaggcc
     6361 ctgcgtgtag ctgccgtaca gcgtgaccgg cttcgccagc gttaccgccg cggtggcgta
     6421 ggcgcgcagc ggtcgatcct ccaggtgggc gatgggcagg cccggtgtga caacgatttt
     6481 gtcgtaactc tcccgctgca cacccactgc cagatcggca cgtccctgcc actgcaggcg
     6541 ataggcggta cccatgctcc acagctgcgc gcgatcgttg gtgcgcgcgc cgtagatgaa
     6601 ggcaggcctt cgtacctgag cgtcggcggc gaccagcgca ggcccgacat ccaccgcatc
     6661 cgagccaccg taggtggcca gcgtgtcgcg accgcgcgca tcgaggatca gctcatgacg
     6721 caggggaccg gtgagatagt gtccggtcag tcgcacttcc ccggaagtgg atgcgatgct
     6781 ctgctcggga tatcccacca cccggtgatc cgcgaggccg accggattgc tgttgagata
     6841 gagatccgaa tagctctccg gctgatcgaa gatcgatcgg aagacaccgg cgctcaggta
     6901 ccagtgtggt gtcagctgcg cggtgatcag cgcgccgtag ttctcactcc agcttgcgcc
     6961 caacgcccag ttctgaccga tgtaaccgtc gaccgtcggc ggtatgaagg cccccgccgg
     7021 gaacacaagg ggcatggttc tcgcgctggt gtactgctgc cagtcgatga atgcccgcag
     7081 cgtgatgcga tcgttcgcag tccactgcgg acttgccccg aaatccagat acttcgcggt
     7141 atagcccttg tcgcccgcgt acggcgagtt ggtcacgatc tgtgagctgg caccgatcgg
     7201 caactgaagg ttacggccga gcaccgggat gtcgccatcg aggctcaggc cgcggttctg
     7261 gaacgggccg aggctggcca ccaccgtggc cgtggcggtg ccgtcgccga cgtgtctgag
     7321 atcgtagtcg acgatgccgg tcggagccgg gaaggggtag ccgacttcgc tgacgccgat
     7381 gcggatcgtg gagccttcga tcacgcggcc ggacaggccg ccctgctgat caaagtacag
     7441 tccgttgatg cgtacgttgc cagcgacctg cggattgaat ccgcgtacgc cgcccggtcc
     7501 atacaggccg atgctctcca gtccgagggt caggccgaac gcatcatcgg caatcgccag
     7561 cggatcgtcg ctggcgtgct gggcgtgcgc gaacggcgcg acggcaagac agagcactgc
     7621 ggcgccggcc ggccaggagg acttgcgtac gcgctgcaag cgaggctctc aggtgccgcc
     7681 attctcctgt atctgcctca tggcatcggg tagctgcggc acgctcagca gttcatcggc
     7741 cagcacgcga cacgtgcgca tgtcgcccgc gaagggtttt ttccgaccgt cgatggcgtc
     7801 gcacatcgcc ggccgcgaga ccggtgcgta ggcctcgccg tcgcagacgt acacgcgcca
     7861 gacaccgaac agcgtgggcg ccaccaatgc cagcaccgcg cagctgctga gcgcgagctt
     7921 tgcgctgaag ccaagcctgc gcgccgcctc gccggtcatg atgtccacga cccgctgctt
     7981 gagttccgcg ccggtcacgc ccgaaagcac gggcagtggc gatccgacgc agtactggca
     8041 ggttttcagg atgctctcgg catacaccgc gcgttcgctg cccagctcca gcaccttctg
     8101 atcgcaggct cgctcgcgct cgcgcatcag tcgcgtgccg agccaccaca ccagcggatg
     8161 aaaccagaac accgcttcga ccaccatatg aatcgcggcc gccagattgt cacggcgacg
     8221 tacgtgacag acctcgtgtg cgatcaccgc ctgcaggtgc gagcgggtga ggtgcgcgga
     8281 aatcccctgg ggccatagca gcaccggccg ccagatgccg aagatgccgg gctcgagcga
     8341 tgcggaacac agcagcaacg tgatcggccg gcggatgccc atttgccgct cgacctgccg
     8401 cagcacgcgc acctcgcgtg cgtcgctgac cacaatgccc gcgcgcgcaa tgcgcgcgat
     8461 cgaacgccag cgcaggaccc acaccgtgag taccacgacg agaccggtgg cccacaccgc
     8521 cagcagcagc gtcgccatga gcgcaaaggg cttcgaggcg tgcggatcgg aagacagcga
     8581 gaacggttca ctgacgcgga tgaccgcgtt ctgccaccag gcttcccggt gccccacttc
     8641 gagcagcgag gagatgctac ggccggcgct gatcagcagc gagaagggaa gcagaaattt
     8701 gatggaggcc gcgaaccata gcccgtggcg cacaccggcc tcgttccggc gcagcaacag
     8761 ccaggcggtc agcgctgccg ccagcgcgac cagggttgac tgccacagat ggttggcaat
     8821 tccggccaca gtgccgctac cgatttcccc cagcagtggt ttcattgtag cgggtccttc
     8881 gc
//
LOCUS       DATMRM010000044        12259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3429172, whole genome shotgun sequence.
ACCESSION   DATMRM010000044 DATMRM010000000
VERSION     DATMRM010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12259
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3429172"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /locus_tag="VNZ06_01190"
     CDS             <1..1580
                     /locus_tag="VNZ06_01190"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HWW19392.1"
                     /translation="FVIVGAGPAGLACALRLKQLKPEASIGVLEKGSTVGSHSISGAV
                     LEPGPLEALIPDWRSTYTGMKVPAVYDEICFLTSKKMYRSPITPPQLNNHGNFIISLG
                     QLTPWMAQKVEAAGVDVFPGFAAAAPLFGADGAVTGVKIGDMGILKDGSQGPNYTPGV
                     EILAGTTVLAEGCRGSVSKVLIDTFKLRAGKSPPTYGLGFKELWQLPPGRVKPGHIRH
                     TAGWPLDSATYGGSFLYQLDNDRLYIGFIVGLDYKDPNLFLFEAFQQFKAHPSIKPLL
                     EGGEILAAGARTVVSSGWQALPKLEMPGALLIGDAAGTLNFPKIKGIHQAIRSGVCAA
                     EHLAEVGSPQGFDVRYRASEGGKELHRVRNFKPGFKRGLLWGTLNFGLEMFAGGHLPW
                     TLTWKSDDASGLKHLDGYTSPDRHWVTRTLPPRDRVSSVFFAGNTHDEHQPVHLKVAD
                     TSICATRCAQEFGNPCQRFCPAAVYEMVDDGKGGKMLRINAANCVHCKACDIKDPYGI
                     ITWMTPEGGSGPNYQNL"
     gene            complement(1642..3144)
                     /locus_tag="VNZ06_01195"
     CDS             complement(1642..3144)
                     /locus_tag="VNZ06_01195"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HWW19393.1"
                     /translation="MSQARVMLDAPLPNTIAELRPEPQLEEDLSALSSSSVGQKAVTL
                     GTIEPPLHTLLRHWSKPAVVAVALLICVLVGSPAVTLSELALAVVAVLISHEIFSPLQ
                     LLSASAPRPAKRRLPRLLIEWGAVVALLLFLGITLKLNNMFPSQVLVGWFALTPVLLL
                     VGDAVSARVAASSSAARQRYVIIGANEVGVELKRRASQNLSMGKFMGFFDFRSLERLP
                     QDTRGQFAGLCKEVAEFVRTHGVNAIYITLPMSKAPRINDMLREFRDTTASIYFVPDI
                     FAYDLVQARCVEINGMPMLSICDTPFHGMQAVRKRAIDVLLSAIGLLVGWPLMIMAAI
                     AIKLSSPGPVLFKQRRYGLNGEEILVYKFRSMRVCEDGSVVVQARKQDDRITPVGRFL
                     RRTSLDELPQLFNVLEGKMSFVGPRPHAVAHNETYRKLISGYMIRHKIRPGMTGWAQV
                     HGLRGETSTVEQMRLRVQYDLDYLRHWSLWLDIKILFKTAVIVIRGRNAY"
     gene            3588..6077
                     /locus_tag="VNZ06_01200"
     CDS             3588..6077
                     /locus_tag="VNZ06_01200"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWW19394.1"
                     /translation="MERAEQLHAEGNYAEAAQICRALAEHDDVAAQLRLAQAYERGEG
                     VLQSFVEAVRWYRAAGERGSTPSMARLGEIYLTGLTAPDTASQAVLERETALLRKLYP
                     EGLSVEQDAAQAALWNARAAEAGNAPAQARLGHQFATGLGIAKDLAVAERWFASSAQL
                     QCAAGELGLGLLYAGAYGERREHTRALEWLERCAARQNATAQLCLAMLLLYGEGVRHD
                     PVRAAQLLQQSADAGQPAAMYHLGDLYRRGHGVEPNASQAESWLRRAASRGYLRARLS
                     LVQLFRAGPEPDLNSAAVLCREAADLGDGEAQYLLGQLYINGEGVPKDPAEAARWLKK
                     AADQNVIPAFERLGALYAQGLGLPQDLQAAADWFHRAEARGDVHAMYQLGTLELGGFG
                     TPRDPRAAREWYRRAAARGSAAACLQLGVLYATDENGHQSYSAAARWYAQAAVHGLAA
                     GRYNLAFLHLRGLGVPQDAARGLKLLHEAVDAGSTQAALALHQLYADGEHVSANAQTA
                     LVWLKRAAGMGNATAACQLAQRLQRAEAPAPPAAEVVALLEGCAASGSVDAQASLGLL
                     YYEGAQVTRDERLAMRWLSRAAQGGNAFAQAWLGDVLIQGRGIPMDQEGAAKWYERAA
                     LQGHLGATAALTSIRMSSGAPSQQMEELFKLWLRAAQQGDATAQRMVGDFYSRGVGVE
                     RSAEHARRWLATAAEQGNASAMASLGVLLLQGATRMSEQVEAVDLLRRAAALGNADAQ
                     YNFGVCLRRGLGVPPDDTQAERQYRAAALKGNVSAQLALGALKAQKAVADSDWNEAVS
                     WYRLAADAGNAVAKRQLDELTARRRGLTAER"
     gene            complement(6079..7332)
                     /locus_tag="VNZ06_01205"
     CDS             complement(6079..7332)
                     /locus_tag="VNZ06_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWW19395.1"
                     /translation="MKYLFIHQNFPGQYRHIVRHLANRPQNDVRFITQPNDNAMPGVT
                     KIIYPADRRSSINCHKYAIEVDRAIHTGATVAEVCARLRDSGFQPDLIVGHSGWGETL
                     FVKDVFPDVPLLSNFEFYYHAQGVDVGFDPEFVSIFHEPSHLRARNAVNLLAFEATDW
                     GHSATHWQRSLYPPEQRSRISVLHEGVDADLACPRRSAHYRLPSGQVLSADDEVVTYV
                     ARNLEPYRGFHVFMRSLPQLLRRRRRAQVVIVGGDGVSYGTPAPPRSTFRELLLQELA
                     GKLDLTRVHFAGTIEHRDYLSLLQISSAHVYLTYPFVLSWSFIEAMACGCLIVASATP
                     PVLEVMRDGQNGLTVDFFSHRQLANRIEEALERQRELRPVRESARATAVRDFDLKRVQ
                     LSRWTRLFDDLIAGRRPDATVLRAV"
     gene            complement(7329..7940)
                     /locus_tag="VNZ06_01210"
     CDS             complement(7329..7940)
                     /locus_tag="VNZ06_01210"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWW19396.1"
                     /translation="MNEPVRARVVLNVGCGVRRASSLPACFDSWKEIRVDSEASVLPD
                     VIADLTDLSSFADASAHAVWAAHCMEHLYEYQIPIALSEFRRVLRNDGFVCVIVPDLQ
                     TVAGYIAQDRLHEPLYQSAAGPVTPHDVLFGFGPAIASGQTTMAHHCGFTPSVLQRCF
                     NGLKYGEIIVRRRSGSLELAAVARVTAASSAAERATLMASLQL"
     gene            complement(7937..11959)
                     /locus_tag="VNZ06_01215"
     CDS             complement(7937..11959)
                     /locus_tag="VNZ06_01215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW19397.1"
                     /translation="MARNDPASRIRPAYVLDPQSPHLQESLRRALRYAGLNPVLGDPA
                     RRAIRQVQQRVGAEDAPGALRLIDQAWRTLPSDGGYLASIYGHLLVWGRRDTVAAHRL
                     LVRAYEMRADADVAALIIWTALETDRLEDVGARLHPALVSHCIEPQSLMQRGANAALL
                     HPHSPFTGWIGLGPGLEFVGALRRDDTAHSVHIQIARGGEFIQPIRTSPNEEVRTFSF
                     RLPDSLRHAGLRFTVHGKPLLGGERHFPPDFALDGRSAASDGRVTGWASSSWRPSQPL
                     AIRVTDDSGRSREGPTHSRYDSVGRWPFSVHLRSAGLTGARFHVSAQLPDGCWQRLPD
                     SPVLLPSSMRPAGRSSTRLGRWTAASAPAGPVQTQRARRCAVVIPVYHGLDETRACLR
                     SVIATVPAHTRIIVVNDASDDADLLAELTTLASTSRIELLHNPVNLGFAASVNRALAL
                     HPTQDVVVLNSDAQVFEGWLERMRRAAYSAPSVGSVTPFSNSGSIATYPHAPNQAIRP
                     EQAAMFHAMAGELHAGQSVEIPVGVGFCLYLRRDCLRDVGDFDAAAFDQGYGEESDFC
                     LRARARGWSHRLAADVFVYHAGGRSFGPRRTALLERGQRLLNLRHPGYDRFISDFIAH
                     DPVHRLRRQLDEKRLNAIAGPGALIVTLALAGGVDRFVAERCRAVRAQGVRPLVLRPA
                     RAGDRGRCELWTDSMVLPDLHYRIPTEIDGITALLRSLSLQSVEIHHFLHLDSKVIEA
                     VRALGLPYDVFVHDYAWVCPQITLIDPGKRYCGEPDVSACNVCLRRLGSQLGEKISVT
                     ALRLRSDSWLRNARRVIAPSSDTATRLQRYFPSLAIAVQPASSPASPPAHAVDVKGGR
                     ALRVALIGAIGQHKGSELLLRCARDARLRGLPIEFVVIGYTDRDRALQRTGKVFVSGR
                     YAEAEVTHLFERERPDAVWMASVWPETWCYALDYALQAGLPVVAFDLGAIAERLRSVR
                     RGTLLPLELSASQINDRLLQLCEETPAMKTPLRPDGKSMQDEGLSASVQLLPLPPGLY
                     LFSVRAAKPSSTPAAGALSLPAVHVGLGPGVRAEQVEFMDGAGNHGAWLFQQGDLLIG
                     KIHGSGATLVLTSVRAPGGEVLSIKVERLEDRASAPESALAANSEPARPEVPVPAPAA
                     MAAAPPAPAVSAHVPMHIAAHIRSRGDVNFTDAPWAGRVAPGLWIESFSVLPMHQFQA
                     QDIEYKALTGSGFETPWLSDNRMCGTRGLSVPLVGFAVRLKPSAEAALYDCEYSGYFQ
                     SGQIVGPLRNGAPCRSTAANDPLEGIQVHLIKRAELTRAKEASPRRRTTSSGPSFGRF
                     RDVPEQTKSARRKSSRHPRP"
     gene            complement(11991..>12259)
                     /locus_tag="VNZ06_01220"
     CDS             complement(11991..>12259)
                     /locus_tag="VNZ06_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19398.1"
                     /translation="QESAFGGAGANVFEFLADQHGGQHVITNFVSGQDKLYLEGYSLS
                     YLQQHGEVSSHGGNTYISLDSGKTTIELKGVSSLHSSDITTHKP"
BASE COUNT         2342 a         3778 c         4024 g         2115 t
ORIGIN      
        1 acttcgtgat cgtcggcgcg gggcctgcgg ggctggcttg cgcgctgcgg ctgaagcagc
       61 tcaaaccgga agcgagcatc ggcgtgttgg agaagggctc gacggtcggg tcgcattcga
      121 tctcgggggc agtgctggag ccggggccgc tcgaggcgct gattcccgat tggcgctcga
      181 cctacaccgg gatgaaggtt ccggcggtat acgacgaaat atgtttcctg acgtccaaga
      241 agatgtaccg ctctccgatt acgccaccgc aattgaacaa ccacggtaac ttcatcatct
      301 cgctggggca gctcacgccg tggatggcgc aaaaagtcga agccgccggc gtggatgtgt
      361 ttccgggctt cgcggccgcg gcgccgctgt tcggtgccga tggtgcggtg accggcgtga
      421 agatcggtga catgggcatt ttgaaggacg gcagccaggg accgaattac accccgggcg
      481 tcgagatcct ggccggtacg accgttctcg cggaaggctg ccgcggctcg gtcagcaagg
      541 tgctgatcga cacattcaag ctgcgggccg ggaagtcgcc gccgacctat ggtctgggct
      601 tcaaggagct gtggcaattg ccgcccggcc gcgtcaagcc aggccacatc cgacacaccg
      661 cgggctggcc gctggacagc gccacctatg gcggcagctt cctgtatcaa ctcgacaacg
      721 atcgactgta catcgggttc atcgtcggtc tcgattacaa ggacccgaac ctgttcctgt
      781 tcgaggcatt tcagcagttc aaggcgcatc cgtcgatcaa gccgctgctg gagggaggcg
      841 aaatcctggc agcgggtgcg cgcacggtcg tttccagcgg ctggcaggcg ctgccgaaac
      901 tcgagatgcc gggcgcgctg ctgatcggcg atgccgccgg cacactgaac ttcccaaaga
      961 tcaaggggat ccatcaggcg atccgttccg gagtgtgtgc cgcggagcat ctggcggaag
     1021 tcggcagccc gcagggcttc gatgtgcgct accgcgcctc ggaaggcggc aaagagctac
     1081 accgggtgcg caatttcaaa ccgggcttca agcgcgggct gctctggggc accttgaatt
     1141 tcggcctgga gatgtttgca ggcggacatc tgccgtggac cctgacctgg aagagcgacg
     1201 atgcctcggg gttgaagcat ctggatggat acacctcgcc cgaccggcac tgggtgacgc
     1261 gcacgctacc gccgcgtgat cgagtctcct cggtgttctt cgccggcaat acgcacgacg
     1321 aacatcagcc ggtgcatctg aaggtcgctg acacgtcgat ctgcgcgacg cgttgtgccc
     1381 aggaattcgg caatccgtgc cagcgcttct gcccggcagc cgtctacgaa atggtggacg
     1441 acggtaaggg tggaaagatg ctgcgcatca acgcggcaaa ttgcgtgcac tgcaaggcct
     1501 gcgacatcaa ggatccctac gggatcatca cctggatgac acctgaaggt ggcagcggcc
     1561 cgaactacca gaacctatag tcgctatgca gatgagctgt ctccaaacgc agacagcgaa
     1621 gaattaccgg gttcgttgca attaatacgc gttacgaccg cgaatgacga tcacggctgt
     1681 cttgaacagg atcttgatgt cgagccacag cgaccagtga cgcaggtagt ccaggtcgta
     1741 ctgaactcgc agacgcatct gttcgaccgt gctggtttcg ccgcgcagtc catgaacctg
     1801 cgcccagccg gtcattccgg gacggatctt gtggcggatc atgtaaccgc tgatcagctt
     1861 gcgataggtc tcgttgtgcg cgaccgcgtg cggtcgcggg ccaacgaagc tcatcttgcc
     1921 ttccaagaca ttgaatagct gcggcaattc gtcgagtgac gtgcggcgca ggaaacggcc
     1981 aaccggcgtg atccgatcgt cctgcttgcg ggcctgcacg accaccgaac cgtcttcgca
     2041 cacccgcatg ctgcggaact tgtagaccag gatttcctcg ccgttcagtc cgtagcggcg
     2101 ctgtttgaac aacaccgggc cgggcgagga gagcttgatg gcgatcgcag ccatgatcat
     2161 cagcggccag cccaccagca ggcctatggc ggatagcagg acatcgatcg cccgtttgcg
     2221 cacggcctgc atgccgtgaa acggcgtatc gcagatcgac agcatcggca ttccattgat
     2281 ttcgacgcat cgcgcttgca ccaggtcata cgcgaagatg tccggtacga aatagatcga
     2341 cgcggtggta tcacgaaatt cgcgcagcat gtcgttgatg cgcggggctt tgctcatcgg
     2401 tagcgtgatg tagatagcgt tcacgccgtg ggtgcgcacg aactcagcca cttccttgca
     2461 caagccggca aactggccac gggtatcctg gggcagccgc tcgaggctgc gaaagtcgaa
     2521 gaagcccatg aatttgccca tgctgaggtt ttgcgacgcc cgacgcttga gctcgacgcc
     2581 aacctcattg gcaccgatga tgacgtagcg ctgacgcgcc gcgctgctgc tcgccgccac
     2641 acgggcgctc acggcatcgc ccaccaacag caaaaccggc gtcagcgcga accaacccac
     2701 gagcacctgg ctggggaaca tgttgttcag cttcagcgta atgccgagga acagcagcag
     2761 cgcgacgacc gcgccccact cgatcagcag ccgcgggagc cgacgcttgg ctggccgcgg
     2821 cgcagacgcg gacagcagct gcaacgggct gaagatctca tgcgaaatca ataccgcgac
     2881 aacggcgagc gcgagctctg acagcgtgac ggccggtgag ccgaccagca cgcagatcag
     2941 cagtgcgacg gcgaccactg cgggcttgga ccaatgcctg agcagtgtat gcagcggcgg
     3001 ctcgatcgtg ccgagcgtca ccgctttctg gcctacggaa gaactcgaga gtgccgacag
     3061 atcttcttcg agttgaggct ccggcctcag ttctgcgatg gtgttcggta gaggagcgtc
     3121 gagcatgact cgggcttgac tcaccagctt acctcccctg ataaagacac aaccatgtta
     3181 gcggagcaaa acccgtgccg acgcgttatg taacagaatg taggcacaaa ggttgacgcg
     3241 gtttgcattg atctcgcggt cgactgcgat gcgtttgcgc aatgtgagca gcgcacacga
     3301 agaactcttg gaccagtttg tcacagataa gttgcagcgc gttgaatcca ttgtttgcct
     3361 tcaacggtgc cgcttggtgc cgagtcatgt gccaaggggt tcgaagccgt gcgcgacgca
     3421 agcttgttga aaattttact cggcaccaaa aaagcgccct cagctctcaa aatgagccac
     3481 ttcgagtccg cagacaggca acggtggcgt ttagcaggca gcgtgcggta ggttgagctg
     3541 tgggaatccg agccattttt tcgcggcgta acgagcgtgc tccgagcttg gagcgtgccg
     3601 agcaactgca cgctgaaggt aactacgcgg aagctgcgca aatctgtcgc gcactcgccg
     3661 aacacgacga cgtggccgca caacttcgac tggcgcaggc gtatgaacgc ggcgagggag
     3721 tgcttcagag tttcgtcgaa gcggtacgct ggtatcgggc tgccggagag cgcggctcca
     3781 caccgtcgat ggcacggctg ggcgaaatct atctcaccgg gttgaccgcg cccgacaccg
     3841 ccagccaagc ggtgctcgag cgcgaaaccg cgttgctcag gaaactctac ccggaagggc
     3901 tgtccgtcga gcaggatgcc gctcaggctg cgttgtggaa tgcacgcgcc gcggaggcgg
     3961 ggaatgcgcc cgcacaggcg cgcctcggac atcagttcgc cacgggactg ggaatcgcca
     4021 aggatctcgc ggtggccgag cgctggttcg cctcgtcggc gcaactgcag tgcgcggcgg
     4081 gcgaactcgg gctcggactg ctgtacgccg gggcttacgg cgaacgccgt gagcatacgc
     4141 gcgcgctgga atggctcgag cgctgtgctg cgcggcagaa cgcgaccgcg caactatgcc
     4201 tggcgatgct gctgttgtac ggcgagggtg tccggcacga tccggtgcgc gcggcgcagt
     4261 tgctgcaaca gtcggcggat gctggccagc ccgccgcgat gtatcacctc ggggatctct
     4321 accgccgcgg ccacggtgtc gagcccaatg catcgcaggc agaaagctgg ctgcgccggg
     4381 ctgcctcgcg cggttacctg cgggcgcgcc tgtcactggt acagctgttc cgcgcagggc
     4441 ctgagccgga tttgaactca gccgcagtgc tgtgcaggga ggcggctgat ctgggcgacg
     4501 gcgaggccca gtatctgctg ggccaactct acattaatgg tgagggcgta ccgaaggacc
     4561 cggcggaggc cgcccgttgg ctcaagaagg ccgccgatca gaacgtcatc cccgcattcg
     4621 agcgtctcgg ggcgctgtac gcgcagggac tgggtctgcc gcaggatctg caagcggcgg
     4681 ccgactggtt tcaccgcgcc gaagcgcgcg gcgacgtcca tgcgatgtat cagctcggga
     4741 ctctggaact ggggggattt ggcacgcccc gcgatccacg ggcggcgcgc gagtggtacc
     4801 ggcgcgcagc agcacgcggt agcgcagcgg cgtgtctgca attgggtgtg ctgtacgcga
     4861 cggatgaaaa cgggcatcag agttattctg ccgcggcccg ctggtacgcg caggccgcgg
     4921 tgcatgggct ggcggccgga cgttataacc tcgcgttcct gcatctgcgc ggtttgggcg
     4981 tgccacagga tgcagcgcgc ggcctgaagc tgctgcacga ggccgttgat gccggtagca
     5041 cccaggcagc gctggcgctg catcagctct atgcggacgg cgagcatgtg tccgcgaacg
     5101 cgcagactgc actggtatgg ctgaagcgcg cggccggcat gggcaacgcg accgctgcgt
     5161 gtcagttggc gcagcgcctg cagcgcgctg aagcaccggc gccgccggcc gcggaggtcg
     5221 tggcactgct ggaaggctgt gcggcgagtg gcagcgtcga cgctcaggcc tcgctcggat
     5281 tgctctacta cgaaggcgct caggtcacgc gcgatgagcg cttggcgatg cgctggttgt
     5341 cgcgggccgc gcaaggtggc aatgcctttg ctcaggcgtg gctgggagac gtactgatac
     5401 agggacgcgg gattccgatg gatcaggagg gcgcggcgaa gtggtacgag cgggcggcgc
     5461 tgcaaggaca tttgggtgcg accgcggctc tgacctcgat ccgaatgtct tcgggcgctc
     5521 cgtcgcagca gatggaggag ctcttcaagc tctggctgcg cgccgcacag caaggggatg
     5581 cgaccgcaca acgcatggtg ggtgatttct attcgcgcgg agtcggggtc gagcgttccg
     5641 ccgagcacgc ccgccgctgg ctcgcgacgg cggcggagca gggtaacgcg tcggcaatgg
     5701 cctcactggg cgtgctgctg ttgcagggcg cgacccggat gtccgagcag gtcgaagcgg
     5761 tggacctgct gcggcgggcg gcggccttgg gcaatgccga cgcgcaatac aacttcggtg
     5821 tctgtctgcg acgcggactc ggggtgcccc cagacgacac gcaggctgaa cggcaatacc
     5881 gcgcggccgc gctgaaaggg aatgtatcgg cccaattggc gcttggagca ctcaaggcgc
     5941 agaaagcggt cgctgactcg gattggaatg aggcagtgag ttggtatcgt cttgcggcgg
     6001 atgccggcaa tgccgtagcc aagcgtcaac tggacgaact gaccgcacgg cggcgtggcc
     6061 tgactgccga gcggtgagct aaacagcccg tagtacggtc gcgtccgggc gccgacccgc
     6121 gatcaggtca tcgaatagcc gggtccagcg cgacagctgc acacgtttga ggtcgaagtc
     6181 gcgaaccgca gtcgcgcgcg cggattcgcg aacgggccgt agctcacgtt gtcgctcgag
     6241 cgcttcttcg atgcgattgg caagctgccg atgagaaaag aaatccacgg tgagcccgtt
     6301 ttgcccgtcg cgcatcacct ccagtaccgg cggtgtggcg gaggcgacga tcagacagcc
     6361 acacgccatc gcttcaatga atgaccagga cagcacgaac gggtaggtca ggtagacgtg
     6421 agcggacgag atctgcaaca ggctcaagta gtcgcgatgt tcgatcgtcc ctgcgaaatg
     6481 tacgcgcgtc agatcgagtt tgcctgccag ctcctgcagc agcagctcgc gaaaggtcga
     6541 tcgcggcggt gccggtgtgc cgtagctgac cccatcgccg ccgacgatga ccacctgcgc
     6601 gcgacgacga cgacgcagta gttgtggcag cgagcgcatg aagacatgaa agccgcggta
     6661 cggctcgagg ttgcgtgcca cataggtgac gacttcatcg tccgcactca acacttgacc
     6721 ggagggcagc cggtagtgcg cgctgcgtcg cggacacgcc agatcggcat cgacgccttc
     6781 gtgaaggaca ctgattctcg agcgctgctc aggcggatag agactgcgct gccaatgcgt
     6841 ggccgaatgc ccccagtcgg tggcttcgaa cgccagcagg ttgaccgcgt tgcgggcgcg
     6901 cagatgcgat ggctcatgga agatcgagac gaattcgggg tcgaacccga catccacgcc
     6961 ctgcgcgtga tagtagaact caaagtttga caggagcggc acgtctggaa acacatcttt
     7021 gacgaacagc gtctcacccc atccgctgtg gccgacgatc agatcaggtt gaaagccgga
     7081 atcgcgcagt cgtgcacaga cctccgctac cgtcgcgccc gtgtgaatcg cgcgatcgac
     7141 ttcgatcgca tatttgtgac aattgatcga gctacgccga tctgccgggt agatgatctt
     7201 ggtcaccccg ggcatggcgt tgtcattggg ctgggtgatg aatcgaacgt cattctgcgg
     7261 acgattggcc agatggcgca cgatgtggcg gtactgcccc ggaaaattct gatggatgaa
     7321 aaggtacttc acagctgcag cgaagccatc agtgtggcgc gctctgccgc gctgctcgcc
     7381 gccgtgaccc tggccactgc cgccagttcg agcgatccgc tgcgccttcg cacgatgatt
     7441 tcgccatact tcaggccatt gaagcagcgc tgcagcaccg agggcgtgaa gccgcaatga
     7501 tgcgccatcg tggtctggcc agatgcgatg gccggaccga acccaaacag cacatcgtgc
     7561 ggcgtgacag gtccggcggc agactgatac agcggctcat gcagccggtc ctgagcgata
     7621 tagcctgcca cggtttgcag atcgggaacg atcacgcaca cgaagccatc gttacgcagc
     7681 acccgcctga attccgacaa cgcaatcggg atctggtact catagagatg ttccatgcaa
     7741 tgcgcggccc acaccgcgtg agcgctcgca tcggcgaacg acgacagatc ggtgagatcc
     7801 gcgatcacat ccggcagcac cgaagcctcg ctgtcgacgc gaatctcttt ccagctgtca
     7861 aagcacgcag gcagactgct cgcgcgtcga acgccgcatc ctacattgag cactacccgt
     7921 gcgcgaacgg gctcattcac ggccgaggat gacgactcga cttgcgtcgg gcggacttgg
     7981 tctgctccgg aacatcacgg aagcggccaa acgagggacc gctactggtt gtccgacgtc
     8041 gcggcgaggc ctctttggca cgggtgagct cggcgcgttt gatcaggtgc acctgaatgc
     8101 cctccagcgg gtcgttcgcg gccgtcgagc gacacggcgc accattgcgc agtgggccga
     8161 caatctgccc ggactgaaag tagccgctgt actcgcagtc atacagcgcg gcttcggcgc
     8221 tgggtttgag tcgcaccgca aagcccacca gcggaaccga caggccgcgt gtgccacaca
     8281 tcctgttgtc gctgagccat ggggtttcga aaccgctgcc ggtcagcgcc ttgtattcga
     8341 tgtcctgcgc ctgaaattga tgcatcggca ggaccgagaa tgattcgatc cacagccccg
     8401 gcgccacgcg tccggcccag ggcgcgtcgg tgaaattcac atcaccgcgt gagcggatgt
     8461 gggctgcgat gtgcatcggt acgtgtgcgc tgacggccgg tgcgggcggc gctgcggcca
     8521 tcgcagcagg agccgggacg gggacttcgg gccgcgccgg ctccgagttc gcggccagcg
     8581 cagactcggg tgcactggcg cgatcctcca gccgctcgac cttgatcgac aacacctctc
     8641 cgcccggggc gcgcacggag gtcagcacca gcgtcgcacc gctgccatgg atcttgccaa
     8701 tcagcagatc gccctgctga aacagccatg cgccgtgatt gcccgcgcca tccatgaatt
     8761 cgacctgctc agcgcgtacg ccagggccaa gtccaacgtg aactgccggc agcgacaacg
     8821 ctcccgccgc cggcgtggac gacggtttgg ccgctctgac agagaacaga tacagtccgg
     8881 gcggaagcgg tagcagctgc accgatgcgc tcaagccctc atcctgcatc gatttaccgt
     8941 cgggcctgag cggcgtcttc atcgctggcg tttcctcgca gagttggagc agtcgatcgt
     9001 taatctgact ggcactgagt tcaaggggca aaagtgtgcc gcggcgcaca gaacgtagtc
     9061 gctcggcgat cgcgccaagg tcgaatgcga cgaccggcag gcccgcctgc aacgcgtagt
     9121 ccagcgcata gcaccaggtc tctggccaga ctgaggccat ccacaccgca tccggacgtt
     9181 cccgttcgaa cagatgcgtc acctcggctt cagcgtagcg cccgcttacg aagactttgc
     9241 cggttctctg caatgcgcgg tcgcgatcgg tgtaaccgat gacgacgaat tcgatgggca
     9301 gcccgcgaag ccgtgcgtcg cgggcgcacc tgagcaacag ctcactgccc ttgtgctgac
     9361 cgatcgcgcc gatcagcgcg actcgcaaag cccgtccgcc cttcacatcg accgcgtgcg
     9421 cgggcggcga ggccggagaa ctcgcgggct gcacggcaat cgcgagagag gggaaatagc
     9481 gctgcaggcg ggtggcggtg tcggaggaag gagcgatcac acggcgtgcg ttgcgcaacc
     9541 aggagtcact gcgcagtctt agggccgtca cggaaatctt ctcgccgagc tgagatccca
     9601 gacggcgcag gcacacgttg catgctgaca cgtccggctc gccgcagtac cgtttgccgg
     9661 ggtcgatgag cgtgatctgc ggacagaccc aggcatagtc gtgcacgaag acgtcgtaag
     9721 gcagtccgag cgccctgacg gcctcgatca ccttcgagtc gagatgcagg aagtgatgga
     9781 tctcgacgct ctgcagagac agacttcgca acagcgcggt aatgccgtcg atctcagtcg
     9841 gaatgcgata gtgcagatcc ggcagcacca tcgagtcggt ccacagttcg cagcgtccgc
     9901 gatcgcccgc acgggccggt cgtagtacca gcggacgcac gccctgcgcg cgcacagcgc
     9961 ggcaacgctc ggcaacgaag cgatccaccc cgccggccaa tgccagagtc actatcaatg
    10021 cgccgggccc tgcgatggcg ttcagtcgtt tttcatccag ctgacgcctc aagcgatgca
    10081 ccggatcgtg cgcgatgaaa tcgctgatga aacggtcgta tcctgggtga cgcagattga
    10141 gcaggcgttg accgcgctcg agcagcgccg tgcgtcgcgg gccaaatgaa cgtccgccgg
    10201 cgtggtagac gaacacatcg gccgccagtc gatgcgacca gccgcgcgcc cgcgcccgca
    10261 ggcagaaatc actttcctcc ccgtacccct ggtcgaatgc cgcggcatcg aagtcaccga
    10321 catccctcag gcagtctcga cgcaagtaca gacagaagcc gacccctacc ggaatctcga
    10381 cggactggcc ggcgtgcaat tcgcccgcca tggcatgaaa catcgccgcc tgttcgggac
    10441 gaatcgcctg attcggggca tgtgggtagg tcgcgatcga gcccgaattg ctgaacggag
    10501 tcactgagcc gacgctgggc gcgctgtagg cggcgcggcg catccgttcc agccagccct
    10561 cgaacacctg tgcgtcggag ttcaacacca cgacatcctg ggtcggatga agggccagcg
    10621 cgcggttgac cgatgcggca aagccgagat tgacggggtt gtgcagcagt tcgatgcgag
    10681 acgtgctggc gagtgtggtc agttccgcca acaagtccgc gtcgtcgctg gcgtcattga
    10741 cgacgatgat gcgcgtgtgc gctggaacgg tggcgatgac cgagcgcagg caggcgcgcg
    10801 tctcgtcgag accgtggtaa accggaatga ccaccgcgca acgacgcgct cgctgggtct
    10861 gaactgggcc ggccggcgcc gacgccgcag tccagcgccc gagtcgcgtg ctcgagcggc
    10921 cagccgggcg catcgaagaa ggcaacagca cagggctatc gggcaggcgc tgccaacacc
    10981 cgtccggaag ctgggccgag acatgaaagc gcgcgccggt cagtcccgcg gatcgcagat
    11041 gaacactaaa cggccagcgg ccaacgctgt cgtaccgcga atgtgttggc ccctcccgcg
    11101 agcggccgct atcgtcggtg acccgtatcg caagcggctg cgagggacgc cacgagctgc
    11161 tggcccatcc ggtaacgcga ccgtcgctcg cggcgctgcg cccgtcgagc gcgaaatcgg
    11221 gcggaaagtg tcgctcgcct cccagcagcg gctttccatg cactgtgaag cgtaggccgg
    11281 cgtgtcgcag gctgtccggt agtctgaagc tgaaggtgcg cacctcttcg tttggcgaag
    11341 tacgaatcgg ctggatgaat tcaccgccgc gcgcaatctg tatatgaact gaatgcgctg
    11401 tgtcgtcgcg acgcagtgca ccgacgaact cgagccctgg acccaagcca atccagccgg
    11461 tgaatggact gtgggggtga agcagggccg cgttggcacc tcgctgcatc agactctgag
    11521 gttcgatgca gtgactgacc aatgccgggt gcaatcgcgc cccgacgtcc tccagccgat
    11581 ccgtctccaa tgcggtccat atgatcagtg cggcgacgtc cgcatcggct cgcatctcgt
    11641 aggcacgcac gagcaaccga tgtgcggcga cggtatcccg tcgaccccac accagcaggt
    11701 gaccataaat tgacgccagg tagccgccgt cagacggcag cgttcgccat gcctgatcga
    11761 tgagtcgaag ggcgccgggc gcatcctccg caccgactcg ctgctgaacc tggcggattg
    11821 cgcggcgtgc cggatcgccg agtacggggt tcaggccggc gtaccgcagc gcccgccgca
    11881 gggattcctg cagatgcggc gactgcgggt ccagaacgta cgccgggcgt atgcgcgacg
    11941 ccggatcatt tcgcgccatt aaacgccgtc cacacggctt aactaccgcg tcagggtttg
    12001 tgggtcgtga tatcggagct gtgcagactg ctgacacctt tgagctcgat cgttgtcttg
    12061 ccgctatcga ggctgatgta ggtgttgccg ccgtgtgagc tgacctcgcc gtgctgctgc
    12121 aggtacgaca gcgaataacc ttccaggtac agcttgtcct gcccggagac gaaattggtg
    12181 atcacgtgct gaccgccatg ctgatccgcc aggaactcaa acacgttcgc gccggcaccg
    12241 ccgaacgcgg actcctgac
//
LOCUS       DATMRM010000045         3235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1264882, whole genome shotgun sequence.
ACCESSION   DATMRM010000045 DATMRM010000000
VERSION     DATMRM010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264882"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VNZ06_01225"
     CDS             <1..879
                     /locus_tag="VNZ06_01225"
                     /inference="COORDINATES: protein motif:HMM:NF045129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWW19399.1"
                     /translation="IEYDFFDPRDLKASLETKALCGLFFAGQINGTTGYEEAAAQGIL
                     AGINAALFTQERPAWSPKRSEAYIGVLVDDLVTRGTSEPYRMFTSRAEHRLLLREDNA
                     DARLTPTGRELGLLDDERWDLFEAKQRAIASQLRRLQGRRFKAGQIPQEWAQRVLNGP
                     PPGRDQSAFDLLRRPEVSHPALIELDASLALDPRLDERLPLQVSLDLEVQASYAGYIE
                     RAADEIERARRYEDLALPSDLDYSALHALSNEVRQKLAAIRPATLGQAARVPGVTPAA
                     VSILLVHLRRHAQRVA"
     gene            968..1396
                     /locus_tag="VNZ06_01230"
     CDS             968..1396
                     /locus_tag="VNZ06_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008050583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWW19400.1"
                     /translation="MATASALLKHKGRVIYSVPPDAPVLEAIKEMAEHSVGALLVMQN
                     ERLMGVVSERDYARKVILKGRSSNETRVSQIMSTPVLTVGPNQSVNDCMRIMTENRVR
                     HLPVVDGERVIGVLSIGDLVRAVVEEQQQTIEQLEHYIRS"
     gene            complement(1402..2862)
                     /locus_tag="VNZ06_01235"
     CDS             complement(1402..2862)
                     /locus_tag="VNZ06_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HWW19401.1"
                     /translation="MRSLLGVLNIFGGLLAWFAALFLLPIMIALVYGELAPLRGFLIG
                     AVITLAAGLLLRVATHRFRYDLKSRDAYLLVSISWLTIAAVATIPLIIDLPGLSFTKA
                     YFETMSGLSTTGATVLTGLDQLPHCINLWRHALSWFGGMGIIVLAIAILPLLGVGGMQ
                     LYRAGAPGTVKDAKLAPRITETARTLGYVYVGMTAACMLALWAAGMPVFDALCHAFST
                     MSLGGFSTHDANIGYFHSPMIELVLMIFMIIAAVNFATHFVALRKGNFAVYKRDPEAQ
                     YMLMVIIASCVGIAIFINFNGVYGTFLDSLRYASFNVISLATTGGFVTADYGSWPVFA
                     PMWMLFLSCLCANTGSTGGGIKMFRSLILVKQSLREMFTLVHPQAVAPLKISGQVVPN
                     GVVYSVLAFLFLYFMTIAALTFLLLISGLDFISSISGIVACINNVGPGLNLVGPGNTY
                     GALTGFQMWVCTAAMFLGRIEIITFAVLFTPTFWRK"
     gene            complement(2870..>3235)
                     /locus_tag="VNZ06_01240"
     CDS             complement(2870..>3235)
                     /locus_tag="VNZ06_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HWW19402.1"
                     /translation="YGELMENHSIDIVISPQTITIGSLLAHVRRGDVVRVHSLRRGLA
                     EAIEAVVHGTQERSQVVGRRLEQIKLPSGASIVAVVRGDSVIMAHHDTTIETEDHVIV
                     FLSDRRHVDAVERLFQTEA"
BASE COUNT          668 a          976 c         1039 g          552 t
ORIGIN      
        1 atcgaatacg atttcttcga tccgcgcgat ctcaaggcct cgcttgagac gaaggcgctt
       61 tgtggactgt ttttcgcggg ccagatcaat ggcaccacgg gctatgagga agccgcggcg
      121 cagggcattc tggccggcat caatgcggcg cttttcaccc aagagcgccc ggcctggagt
      181 ccgaagcgca gcgaagccta catcggcgtc ctggtggacg acctggtgac ccgtggcacc
      241 tcggaaccct accgcatgtt caccagtcgg gccgaacacc ggctgctgtt gcgggaggac
      301 aatgcggatg cgcgcctgac accgaccggg cgcgagctcg gtctgctcga cgatgagcgc
      361 tgggacctgt ttgaagccaa gcagcgggca atcgcaagcc agttgcggcg gctgcagggg
      421 cggcgcttca aggcggggca aatcccgcag gagtgggcgc agcgcgtgct gaacgggccg
      481 cctccgggtc gcgatcagag tgcgttcgac ctgctgcgcc gcccggaagt cagccaccca
      541 gcgctcatcg agctggacgc ctcgctggcc ctggatccac ggttggatga gcgcctgccg
      601 ttgcaggtga gtctggatct ggaagtgcag gcgagctatg ccggctacat cgagcgggct
      661 gccgatgaga ttgagcgcgc gcggcgctat gaggatctgg cgcttccctc ggatctcgac
      721 tattcggccc tccatgcctt gtcgaacgaa gtgcgacaga aactggctgc gatccgtccg
      781 gcgacgcttg gtcaggccgc tcgggtcccc ggtgtcacgc cagcggcggt gtcgattctg
      841 ctggtccacc tgcgccgtca cgctcagcgc gtcgcctaaa ggccaacgga ggactgacgc
      901 tccggccgga tcgcgttaag atcagccggg tgcgctcctt aggctctggt accaggaagg
      961 aggagtcatg gcgactgcgt ctgcgttgct caagcacaag ggccgtgtga tctacagcgt
     1021 gccgccggat gcaccggtgc tcgaggcgat caaggagatg gccgagcaca gtgttggcgc
     1081 gttgctggtc atgcagaacg agcgtctgat gggcgtcgtt tccgagcgcg actacgcgcg
     1141 caaggtcatt ctcaaagggc gctcttccaa tgaaacgcgg gtctcacaga tcatgagcac
     1201 accggtgctc acagtgggtc cgaatcaatc cgtgaacgac tgcatgcgca tcatgaccga
     1261 gaaccgggtt cgccacctgc cggtggtgga cggcgagcgg gtcatcggtg tgctgtccat
     1321 tggcgatctg gtacgcgccg tggtggaaga gcagcaacag accatcgagc agctcgagca
     1381 ctacattcgc agctagcgcg ttcatttgcg ccagaaagtt ggcgtgaaca gcactgcgaa
     1441 cgtgatgatc tcgatgcggc ccaggaacat cgcggcggtg cacacccaca tctgaaaccc
     1501 ggtgagtgcg ccgtaggtat tgccgggacc cacgaggttg aggcccggtc ccacattgtt
     1561 gatgcacgcc acgatcccgg agatcgacga gataaagtcc aggccggaga tcagcagcag
     1621 gaaagtcagt gccgcgatcg tcatgaaata aagaaacaaa aacgccagta ctgagtacac
     1681 cacgccattc ggtaccacct gaccggagat cttcagtggc gccaccgcct gcgggtgtac
     1741 cagcgtgaac atctcgcgca gtgactgctt caccagaatc agcgagcgga acattttgat
     1801 gccgccgccg gtggagccgg tattggcgca caggcagctc aggaacagca tccacatcgg
     1861 cgcgaatacc ggccagctgc cgtagtcggc ggtgacgaag ccgccggtgg ttgccagcga
     1921 gatcacgttg aacgacgcat agcgcagcga atcgaggaac gtaccataga cgccattgaa
     1981 gttgatgaag atcgcgatgc cgacgcaact ggcaatgatg accatcagca tgtactgcgc
     2041 ctccggatcg cgcttgtaca ctgcgaaatt gcccttgcgc agcgcgacga agtgcgtggc
     2101 gaagttcacc gcggcgatga tcatgaagat catcagcacc agctcgatca tcggcgagtg
     2161 gaagtagcca atgttggcat cgtgtgtaga gaaaccgccc agcgacatgg tggagaaggc
     2221 gtggcacagc gcatcgaaca ccggcatgcc ggcggcccac agcgccagca tgcaggctgc
     2281 ggtcatgccg acgtagacgt agccgagggt gcgcgccgtt tcggtaatcc tgggcgccag
     2341 tttggcgtcc ttgacggtac cgggcgcgcc ggctcggtag agctgcatac cgccgacacc
     2401 tagcagcggc aggatcgcga tcgccagcac gatgatgccc atgccgccga accagctcag
     2461 cgcgtgccgc cacaggttga tgcaatgggg tagctgatcg agtccggtca acaccgtggc
     2521 gccggtggtc gacagacccg acatggtctc gaagtacgcc ttggtgaagg acagtcccgg
     2581 caagtcgatg atcagcggga tggtcgcaac cgctgcgatc gtcagccagc ttatgctgac
     2641 tagcaggtag gcgtcgcgcg acttgaggtc gtaccggaaa cgatgagtgg ccacgcgtag
     2701 cagcaatccc gcagcgagcg tgatcaccgc accgatcagg aaaccgcgca gcggcgccaa
     2761 ttcaccatac accagcgcaa tcatgatcgg cagcagaaac agggcggcga accaggcgag
     2821 cagtccgcca aagatattga gaacgcccag cagcgagcgc atcgcgatcc taggcctcgg
     2881 tctggaacaa tcgctcgacc gcgtccacgt gacgtcgatc ggacaggaac acgatcacgt
     2941 gatcttcggt ttcgatcgtg gtgtcatggt gcgccatgat cacgctgtca ccgcgcacga
     3001 ccgcgacgat gctggcgccg gaaggcagct tgatctgctc taggcggcga ccgacgacct
     3061 gcgatcgttc ctgagtgccg tgcacgacag cctcgatcgc ttcagccaat ccacggcgca
     3121 acgaatgcac gcgcaccacg tcgccgcgtc gcacatgcgc cagcaacgaa ccgatggtga
     3181 tggtctgcgg cgagatcacg atgtcgatac tgtgattttc catcagctcg ccgta
//
LOCUS       DATMRM010000046         3079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_333873, whole genome shotgun sequence.
ACCESSION   DATMRM010000046 DATMRM010000000
VERSION     DATMRM010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333873"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VNZ06_01245"
     CDS             <1..186
                     /locus_tag="VNZ06_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HWW19403.1"
                     /translation="FLSDEFSLVDASIAPILWRMRHYDIEMPPQAQALIKYASTVFAR
                     PSFRNSLSDAELQMGAN"
     gene            186..611
                     /locus_tag="VNZ06_01250"
     CDS             186..611
                     /locus_tag="VNZ06_01250"
                     /inference="COORDINATES: protein motif:HMM:NF016286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HWW19404.1"
                     /translation="MLGKGGGGVSRRPYLLRAMHEWITDSGHTPHVIVDATRAGVEVP
                     GAYIKDGKIVLNLSTSATQRLQLKNDWIEFDARFAGVVHHVRVPMHAVLGVYARETGE
                     GMVFSETEKEPTPPEPPNQPDKAPLTPTEGRRAKLTVVK"
     gene            complement(604..960)
                     /locus_tag="VNZ06_01255"
     CDS             complement(604..960)
                     /locus_tag="VNZ06_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HWW19405.1"
                     /translation="MRSARKQLGQLAEERAAQHLQQHGFVILVRNFRARTGELDIVAR
                     RDHLLVIAEVRLRSSHEFGGADASITRDKQLRILRAARYLLICRPSLKTLTVRFDALL
                     LSSQDGPIQWIENAFT"
     gene            complement(1092..2975)
                     /locus_tag="VNZ06_01260"
     CDS             complement(1092..2975)
                     /locus_tag="VNZ06_01260"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HWW19406.1"
                     /translation="MNQPKLYAAIQRASALGLALLLASCALVGPKVSERAGTEQSAET
                     AARKGDHEQAANTYERIAENRSASEQTDLRLSAAREWLAANRPAEAMRVLNALGGTLS
                     PAQSLERSLIDAESALLSNRAAEAWQKINAIAEATAANSGSRYYPLKMRIALATDRPI
                     DGVRAEMAAERYSVNASETTQWRSQLLAGLRAARDRGVKLDPASSSDPIVRGWLELGA
                     IATDTRGVSLNAAAESAAWRAKYPNHPALEILPQAIPAPLVSAAPGGRIALLLPLTGA
                     AASQAAIVRDGFQSAYDQLPQATRPDLRLYDTGALPPAEAIAEAHSAGSNFIVGPLTR
                     EDVAAVAALGSQSVPVLALNYLPADRQAPGGMYQFALSPENEARLVARRLIADGHHRG
                     IALVPRGDWGNRVIEAFARELAAGGGSLMAQVEYDSSEKDYGYQIQSVLRVSDSEARL
                     QRLQTVLGTKLNFEPRHRADIEFIFMVTERATNARLMLPQLKFFYAGDIATYALSTAY
                     EPDSIDANRDIDGLIYPDMPWMVSDDGSIEAIRTQISQTWDGKAAWHSRLFAFGYDAC
                     QLAIAMSGARRNPAEVQVAGLSGQLRLDPNGRVLRDLIWVQIRDGDPRRLTSSIVNGA
                     NID"
BASE COUNT          548 a          974 c         1007 g          550 t
ORIGIN      
        1 ttcctgagcg atgagttttc gctggtggac gccagcatcg ctccgatcct gtggcgcatg
       61 cgtcactatg acatcgagat gccaccgcag gcgcaggcgc tgatcaaata cgccagcacg
      121 gtctttgcgc gaccatcgtt ccgtaacagt ctgtcggacg ccgagctgca aatgggggcc
      181 aactaatgct cgggaagggc ggcggtggcg tatcgcgccg gccctattta ctgcgggcga
      241 tgcacgagtg gatcacggat agcggccaca ccccacacgt gatcgtcgat gcgacgcgcg
      301 caggggtcga agtccccggt gcgtacatca aggacggcaa aatcgtcctg aatctgtcta
      361 ccagcgccac gcagagactg cagctcaaga acgactggat agaattcgat gcccgctttg
      421 ccggcgtcgt gcatcacgta cgcgttccga tgcacgcggt gctgggggtt tatgcgcgtg
      481 agaccggcga agggatggtg ttttcggaga ccgagaagga gcccactccg ccggagccac
      541 cgaatcagcc ggataaagcc ccgctgaccc ccaccgaagg gcggcgcgcg aagctgaccg
      601 tcgtcaagta aacgcgttct cgatccactg gatcggtccg tcctggctgc tcagcagcaa
      661 ggcatcgaac cggaccgtca gcgtcttcaa ggaagggcgg caaatcagaa gatagcgcgc
      721 agcgcgcagg atgcgaagtt gtttgtcgcg cgtgatgctg gcatcggcgc cgccgaactc
      781 atggctggaa cgcagtcgaa cttcggcaat caccaggagg tgatcgcgac gcgctacgat
      841 gtcaagctcg ccggtgcgag cgcggaagtt gcgcacgaga atgacgaagc catgctgttg
      901 aaggtgctgt gcggcacgtt cctcggccag ctgccccagt tgtttgcgcg cgctcctcac
      961 acgagcaggg tgcgactgcg cacggccatt tcacaaggcg gtaacgctac gcgttggtgc
     1021 gaatactcgc acccggcaaa gcaacttcgt ggcgacccga cgcgcgaccg cgctgacgct
     1081 ccgaagcgaa cctagtcgat gttggccccg ttgacgatgc tgctcgtgag gcgcctggga
     1141 tcgccgtcgc ggatctgcac ccagatcaga tcacgcagga cacgcccatt ggggtccaaa
     1201 cgcagttgtc cgctcagccc ggccacttgc acctccgcag gattgcggcg cgcgccggac
     1261 atggcaattg cgagctgaca ggcgtcatag ccaaaggcga acagtcggct gtgccaggcg
     1321 gccttgccgt cccaggtctg actgatctgc gtgcggatcg cctcgatgct gccatcatcg
     1381 ctcaccatcc acggcatgtc cggatagatg agcccgtcga tatcgcggtt ggcgtcgatc
     1441 gaatccggtt cgtacgcggt ggacagcgca taggtggcaa tgtcgccggc gtaaaagaac
     1501 ttcagctgcg gcagcatcag tcgagcgttg gtggcgcgtt ccgtgaccat gaaaatgaac
     1561 tcgatgtccg cccggtgccg gggttcgaag ttcagctttg ttccgagtac ggtctgcagg
     1621 cgctggagtc gcgcttcgct gtccgagacg cgcaggacgc tctgtatctg atagccgtag
     1681 tccttctcgc tcgaatcgta ttcgacctgc gccatcaggc tgccgccgcc cgccgccagc
     1741 tcgcgcgcga acgcctcaat cacgcgattg ccccaatcac cgcggggtac cagcgcgatg
     1801 ccgcggtgat gaccgtcagc aatcagacgc cgcgctacca gccgggcttc gttctcgggc
     1861 gagagcgcga attgatacat gccgcctggg gcctgacggt cggcgggcag gtagttgagc
     1921 gccagcaccg ggactgactg cgagccgagc gcggcgaccg ccgcgacatc ctcgcgcgtc
     1981 aacggtccga caatgaaatt gctgccagcg ctgtgagctt cagcgatcgc ttccgccggt
     2041 ggcaacgcac cggtgtcgta caggcgcaga tccggtcgtg tcgcctgcgg caattgatcg
     2101 taggcgctct ggaagccatc gcgcacgatg gctgcctggc tggccgcggc accggtcagc
     2161 ggcagcagca gcgcgatgcg accgccgggg gcggcgctga ccagtggtgc cggaatggcc
     2221 tgcggcagga tttcgagcgc cggatgattc gggtatttgg cgcgccaggc cgccgattcc
     2281 gccgctgcgt tcagcgacac gccgcgagtg tcggtcgcga ttgcgcccag ttccagccat
     2341 ccgcgcacga tcggatcgga gctgctggcg gggtcgagtt tcacgccgcg atcccgcgcc
     2401 gcccgcagtc ccgccaatag ctggctgcgc cactgcgtgg tttcgctggc attgacgctg
     2461 tagcgctccg cggccatctc ggcgcgcacg ccatcaatcg gccggtcggt cgccagcgcg
     2521 atgcgcatct tcaacgggta gtagcgcgag ccggaattgg cggcagtcgc ttcggcaatg
     2581 gcgttgatct tctgccacgc ctcggcggcg cggttggata gcagcgcgct ttcggcgtcg
     2641 atcagcgaac gctccaggct ctgagcagga ctcaaggtcc cacccagtgc gttcagcaca
     2701 cgcatcgcct cggcggggcg gttggctgca agccactcac gggcagcgct caggcgcaga
     2761 tccgtctgct ccgaggcgga acgattttca gcgattcgct cataggtgtt ggcggcttgc
     2821 tcgtgatcgc ccttgcgcgc ggccgtctcg gccgactgct cggtcccggc acgctcggag
     2881 actttaggtc cgaccagcgc gcatgaggcg agcaatagag ccaagccgag cgccgatgcg
     2941 cgttggatgg cagcgtagag tttcggttga ttcatcgaca acacgctccg caagcaggag
     3001 actgcgctcg gcacttcagg gagaattata tcgtggctgg ccgtctcgac gtggtcgcaa
     3061 cacccatcgg caatctggc
//
LOCUS       DATMRM010000047         4772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4052727, whole genome shotgun sequence.
ACCESSION   DATMRM010000047 DATMRM010000000
VERSION     DATMRM010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4052727"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VNZ06_01265"
     CDS             <1..497
                     /locus_tag="VNZ06_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWW19407.1"
                     /translation="DKADIFVTATGNYHVITRAHMEHMKNQAIVCNIGHFDNEIDVAS
                     LKSLKWENIKPQVDHIIFPDGKRIILLAEGRLVNLGCGTGHPSYVMSSSFANQTMAQI
                     ELWSNHSKYKIGVHILPKLLDEKVAKLQLAKLNAQLTQLSDQQAQYIGVSRQGPYKSD
                     HYRY"
     gene            753..1538
                     /locus_tag="VNZ06_01270"
     CDS             753..1538
                     /locus_tag="VNZ06_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWW19408.1"
                     /translation="MTPDVRILHISDPHLFTDPKGSLRGVETLTSLRQVLAHAMRRHR
                     ALDAVLCTGDIVNDDAGAYLHFTRELGALGQPVYCVPGNHDNPGVMRRALSYAPFQVG
                     GYVDLGTAWRIVLLDSCIPGKAAGRISGSELRALETALAGTDRYTMVCMHHHPVGMAS
                     HWLDAVGIENADELFKILDAHSRVRVVSWGHVHQCFDGRRQGVRLLATPSTGGQFLPL
                     SDEFAIDQRPPAYRRLTLQSDGTIETEVVWVDEAASALVSASA"
     gene            complement(1545..2117)
                     /locus_tag="VNZ06_01275"
     CDS             complement(1545..2117)
                     /locus_tag="VNZ06_01275"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWW19409.1"
                     /translation="MGAEIALFPLGTVLVPGGLLPLRIFEARYVDMVSRCMRQNETFG
                     VVLIKEGTETGRVAQTAAVGTTARIIDFQTLEDGLLGLLCRGETRFRIERRSQQGDGL
                     NLASVEWNPDPNGVPIDPKYQSLVTVLRDALSRLSNLSQFIEPRYEDATWVSYRLAEL
                     LPLPVNLQQKLLEMNEPNERLELLAPLIEP"
     gene            complement(2107..3498)
                     /gene="mpl"
                     /locus_tag="VNZ06_01280"
     CDS             complement(2107..3498)
                     /gene="mpl"
                     /locus_tag="VNZ06_01280"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008843230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HWW19410.1"
                     /translation="MHVHILGICGTFMGGIAAIARAAGHRVTGSDRNVYPPMSTQLQA
                     LGIELMEGYEAGQLGLNPDVVVVGNVMTRGMPVIEAMLDRGVSYTSGPEWLASAVLRE
                     RWVLAVAGTHGKTTTSSMLAWILQDAGLSPGFLIGGIPGNFGESARLGEAPFFVIEAD
                     EYDTAFFDKRAKFVHYRPRTAVLNNLEYDHADIYPDLAAIERQFHQLVRTVPRIGRIV
                     CNGADPALARVLAQGCWTAVEKFSQSGGEGVQWSAGVPADADFSHFEVLGLGHSLGEL
                     QWQLLGAHNVDNALAAIAAARHAGVPVERSIDALKRFAGVARRMQLRGESDGVKIYDD
                     FAHHPTAIATTIDGLRRRVGRERIVAVLEPRSNTMELGVHRQTLGASLAAADEVWLYA
                     PANVGWDVRESVATLGARAHVSDDMDGLLDSLTRTLRRGDHALIMSNGGFGGLHTRLL
                     QSLKRRVDGDRGR"
     gene            3543..4622
                     /gene="rnd"
                     /locus_tag="VNZ06_01285"
     CDS             3543..4622
                     /gene="rnd"
                     /locus_tag="VNZ06_01285"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HWW19411.1"
                     /translation="MAIIGDPNTLVQFSHELSQQPAIGLDTEFMRERTYFARLCLLQL
                     SFGARCVCVDTLALPDLASLRATMGDRNIIKVLHAARQDLEVLAPATGQVVGLFDTQV
                     AAALIGLPAQVGYGDLVHQLLGQTLHKAQTRTDWSHRPLTQAQIDYALDDVRHLLPLR
                     ERLTQRLRTLGRWAWFEEEMAQLDGAGPFVTDPEEAWHRIKGVGELDAARQTLARALA
                     AWRERRAISADRPRSWILPDQVLRDLVIQAPRTMQQLGRIEELPEGVRTHSGPELLAV
                     IADLQLPAQLPPLPRRTRREPAENDRVRKLAQVTQQAGRELGIAPEILATRRELERLV
                     AGARDGAPMSGWRQAVIGNRLLQAL"
BASE COUNT          888 a         1563 c         1451 g          870 t
ORIGIN      
        1 ccgacaaggc cgacatcttc gtcactgcga ccggcaacta tcatgtgatc acgcgggcgc
       61 acatggaaca catgaagaac caggcgatcg tgtgcaacat cggccacttc gacaatgaga
      121 tcgacgtggc gtcgctcaag tccttgaagt gggaaaacat caagcctcag gtcgatcaca
      181 tcatcttccc ggacggcaag cgcatcatcc tgctggcgga aggccggctg gtgaatctgg
      241 gctgtggaac cgggcacccg agctatgtga tgtcctcctc attcgccaac cagacgatgg
      301 cgcagatcga actgtggagc aatcattcca agtacaagat cggcgtgcac atcctaccga
      361 agctgttgga cgaaaaagtc gccaagctgc agctggcgaa gctcaatgcg cagctgacgc
      421 agctgagcga tcaacaggcc cagtacatcg gtgtcagtcg ccagggtccc tacaagagcg
      481 atcactaccg ttactgacta agttcaaacg ctgtaggaat tcgctgattc tgtcggcgaa
      541 ttccgacagt aggcgcttca caaattcggc gcgcatcctc cgtgcgcgcc gaactttttt
      601 cggccgggga acatccaact gccagtgttt ttaacatctg acgcaagtcg gagtgcgatt
      661 tgagcgctgt gtcactcgat cgttccgtgg agattttctg cgcatgatcc acatcactcg
      721 aacacttcga cggcgcgcgt aagatccgcc caatgacgcc cgacgttcgc atcctgcaca
      781 tctcagatcc gcatttgttc actgacccga aaggcagtct gcgcggcgtt gagaccttga
      841 cctcgctgcg gcaggtgctc gcgcatgcga tgcgccgcca cagggcgctg gatgcggtgc
      901 tatgcaccgg cgacatcgtc aacgatgatg ccggcgccta cctgcacttc acacgtgagc
      961 tcggcgctct cggccagccg gtgtactgcg ttcccggcaa tcacgacaac ccgggtgtga
     1021 tgcgtcgcgc actttcgtac gcaccgtttc aggtcggagg ttatgttgac ttgggcaccg
     1081 cctggcgcat cgtgctgctg gatagctgca tcccgggcaa ggccgccggc cgcatcagtg
     1141 gctcggaatt gcgggcgctg gaaaccgccc tcgcgggcac agaccgctat acgatggtgt
     1201 gcatgcatca ccatccggta ggcatggcga gccactggct cgatgcggtc ggcatcgaga
     1261 atgccgatga gctcttcaag atcctcgacg cccattcacg cgtgcgggtg gtgagctggg
     1321 gacacgtgca tcaatgtttc gatggccgcc gtcagggtgt acggctgctg gccactccat
     1381 ccaccggtgg tcagttcctg ccgttgtcgg atgagttcgc catcgaccag cgtccgccgg
     1441 cctatcgtcg cctgacgctg cagagcgatg gcaccatcga gacggaagtt gtctgggtcg
     1501 atgaggccgc cagcgcgctg gtgagcgcct cagcctgaca ttgtctacgg ctcgatcagc
     1561 ggcgccagca attccaggcg ctcgttcggc tcgttcatct cgagcagttt ttgctgcagg
     1621 ttgactggca gcggtagtag ttccgccagt ctgtagctca cccaggttgc gtcctcgtag
     1681 cgcggctcaa tgaactgact caagttcgac agtcgcgaca gcgcgtcgcg cagcacggtc
     1741 accagagatt ggtatttcgg atcaataggg acaccattcg gatcgggatt ccattcgacg
     1801 ctggcgaggt tcaacccgtc gccttgctgg ctgcgccgct cgatccgaaa gcgcgtctca
     1861 ccgcggcaca gcagacccag caggccatcc tccagggtct ggaagtcgat gattcgcgcg
     1921 gtggtgccta ccgccgcggt ctgcgccacg cggccggtct cggtgccttc tttgatgaga
     1981 acgaccccaa aggtctcgtt ctgccgcatg cagcggctga ccatatcgac atagcgtgct
     2041 tcgaagattc gaagtggcaa cagtccgccc ggaactaaca ccgttccaag cggaaacagg
     2101 gcgatctcag cgcccacgat caccgtccac gcgacgcttc aaagactgca gcaatcgcgt
     2161 gtgcaatccg ccgaagccgc cgttgctcat gatcagcgcg tggtccccgc gtctcagggt
     2221 gcgcgtcagc gaatcgagca gaccgtccat gtcatcgctc acatgcgccc gcgctccgag
     2281 cgtggccact gactcgcgca cgtcccagcc cacattggcc ggtgcgtaca accatacctc
     2341 gtcggctgcg gccaacgacg cccctagcgt ctgccgatgc acaccgagtt ccattgtatt
     2401 ggagcgcggc tcgagcaccg cgacgatgcg ttcgcggccg acccgccgtc gcaagccgtc
     2461 gatcgtcgtg gcgatcgccg tcgggtgatg tgcgaagtca tcgtagatct tcacgccgtc
     2521 ggactcgccg cgcagctgca tgcgtcgcgc cacccccgcg aagcgcttga gggcatcgat
     2581 gctgcgctcc accggcacgc cagcgtgacg cgcggcggcg atcgccgcga gcgcgttgtc
     2641 aacgttgtgt gctcccagta gctgccattg caactcgccg agtgagtgcc cgagcccgag
     2701 cacctcgaag tgcgagaaat ccgcatcggc cggtacccct gcactccact gcaccccttc
     2761 gccacccgac tgcgagaatt tctccaccgc agtccagcag ccctgtgcca gcacccgcgc
     2821 aagcgccgga tcagcgccgt tgcagacgat gcggccaatg cgcggcacgg tacgcaccag
     2881 ctggtgaaac tggcgctcga tcgcagccag gtccggatag atgtccgcgt ggtcgtattc
     2941 gaggttgttc aagaccgcgg tgcgcggccg gtagtgaacg aacttggcgc gcttgtcgaa
     3001 gaacgcggtg tcgtattcat ccgcctcgat cacgaagaac ggtgcttctc ccaatcgcgc
     3061 gctttcgccg aaattgccag gtatgccgcc gatcagaaat cctggtgaaa gtccggcatc
     3121 ctgcagaatc cacgccagca tgctcgaggt agtggtcttg ccgtgtgtgc cggccaccgc
     3181 cagcacccaa cgctcgcgca acaccgcgct cgcgagccat tccggccccg aggtatagct
     3241 tacgccgcgg tcgagcatcg cctcgatcac cggcatacca cgggtcatga cattgcccac
     3301 caccaccaca tccgggttca gaccgagctg tccggcctcg tagccctcca tcagttcgat
     3361 acccagtgcc tgcagctgcg tgctcattgg cgggtagacg ttgcgatccg agccggtgac
     3421 acgatgaccg gcggcccgcg caatcgcggc aatcccaccc atgaaagtgc cgcagattcc
     3481 gaggatgtgt acgtgcatgc tggcggcagg ctcctggttc aattggcagt aagatccgcg
     3541 ctatggcgat tatcggcgac ccgaacactc tggtgcagtt ctctcacgag ctgtcccaac
     3601 agccggcgat tggattggac accgagttta tgcgtgaacg gacttacttc gcgcgacttt
     3661 gcctgctgca gctcagcttt ggcgctcgct gcgtctgcgt cgatacgctg gcgctgccgg
     3721 atctggccag tctgcgcgcg acgatgggcg accggaacat catcaaagta ctgcacgccg
     3781 cacgccagga tctggaggta ctcgcacccg caaccggaca ggtcgtcggc ttgtttgata
     3841 cgcaggtcgc cgcggcgctg atcggtctgc ctgcccaggt cggatacggc gatctggtcc
     3901 atcagctgct ggggcaaacc ctgcacaagg cgcagacccg caccgactgg tcgcaccggc
     3961 cactgaccca ggcccagatc gactacgcgc tggacgacgt tcggcacctg ctgccgctac
     4021 gcgagcgcct gacgcaacgg ctgcgcacgc ttggacggtg ggcctggttc gaagaggaga
     4081 tggcgcaact cgacggcgcc ggtccttttg tcaccgatcc ggaagaggcc tggcaccgga
     4141 tcaagggggt cggcgagctc gacgcggcgc gccagaccct ggcacgcgcc ctggccgcct
     4201 ggcgcgaacg acgtgccatc agcgccgacc ggccgcgcag ctggatcctg cccgatcaag
     4261 tattgcgcga cctggtgatc caagcgccac gcaccatgca gcagctcggc cgcatcgaag
     4321 agctgccgga aggcgtacgc acccacagcg gcccggagct gctggcggtc atcgccgact
     4381 tgcaactgcc ggcgcagctg ccaccgctgc cgcgtcggac gcgccgtgag cccgccgaaa
     4441 acgaccgggt ccgaaagctg gcgcaggtca ctcaacaggc gggacgggaa ctgggcatcg
     4501 cgccggagat tctggcgacc cggcgtgagt tggaacggct ggtggctggc gctcgtgatg
     4561 gtgcgccgat gtcaggttgg cgccaggcgg tgatcggcaa ccgcctgctc caggccctgt
     4621 aggcctcagg aaaccgctag ctgccagcgc cgcgcagcgc tgcggccgac ggcacggcat
     4681 cgagcaaggc aaacaacgcc gcggtgaccc gctccacgtc ttgttgagca ttgatggtac
     4741 gcaacagtcc ctggtcgccg tagaacttga tc
//
LOCUS       DATMRM010000048         5658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2810850, whole genome shotgun sequence.
ACCESSION   DATMRM010000048 DATMRM010000000
VERSION     DATMRM010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2810850"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="VNZ06_01290"
     CDS             complement(<1..157)
                     /locus_tag="VNZ06_01290"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004644206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HWW19412.1"
                     /translation="MIPGEVIVGDGELTLNDQRETRRLTVSNTGDRPIQVGSHYHFYE
                     TNAALKFD"
     gene            complement(165..467)
                     /gene="ureA"
                     /locus_tag="VNZ06_01295"
     CDS             complement(165..467)
                     /gene="ureA"
                     /locus_tag="VNZ06_01295"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008054002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HWW19413.1"
                     /translation="MELSPREKDKLLLFTAALLAERRKARGLRLNYPEAVAFISAAIL
                     EGARDGRSVAELMGYGTTLLGRSDVMEGVPEMIAEVQVEATFPDGTKLVTVHHPIP"
     gene            complement(480..1322)
                     /locus_tag="VNZ06_01300"
     CDS             complement(480..1322)
                     /locus_tag="VNZ06_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HWW19414.1"
                     /translation="MTALKEEAATGWQGLLELRFAADESRTRLAHRLHRGPLMVQRAF
                     YPDGPVCHVYVIHPPGGVVSGDELSLQVEVGPGALALLTTPAAGKFYRRYGACQARLT
                     QVLRVEGGTLEWLPQENICYPNADATLATVVRLQGDCHFFGWEIACFGLPASQLDLGK
                     GRIRQCLELWHDDAALVLERVTIDATATRARWGLAGRVSAGTLLAFPACAAHLEQARD
                     IARSWNQDADLLLACTLIDKTLCCRAYAARADRLKQAFVDLWSGLRMTLLGRAAVAPR
                     IWKT"
     gene            complement(1319..2014)
                     /gene="urtE"
                     /locus_tag="VNZ06_01305"
     CDS             complement(1319..2014)
                     /gene="urtE"
                     /locus_tag="VNZ06_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HWW19415.1"
                     /translation="MLEITGLNQFYGGSHTLWDVTLDVPTGSCTCLMGRNGMGKTTLL
                     RCIMGLLPASSGQILYAGQDLRALPAEARAPMGIGYVPQGREIFSQLTVEENLQTGLA
                     ARRTRLREIPEQLFTMFPVLKQMLRRRGGDLSGGQQQQLAIARALVLDPKLLILDEPT
                     EGIQPNIVHEIGNVIQRLNREAHLTVLLVEQKLPFARRVASDLRILDKGRLVVSGKIE
                     TLTDELVRTHLTV"
     gene            complement(2018..2866)
                     /gene="urtD"
                     /locus_tag="VNZ06_01310"
     CDS             complement(2018..2866)
                     /gene="urtD"
                     /locus_tag="VNZ06_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005261534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HWW19416.1"
                     /translation="MIRQVLNHFSGRSRSAMPARVGELLEANRIEAGPDASHGIILYL
                     DDITVSFDGFRALNALTLYIEVGELRCIIGPNGAGKTTMMDVITGKTRPDTGSAWFGQ
                     TIDLLSLTEPEIAQAGIGRKFQKPTVFEHHTVFENLELAMAGDKSVWKWLYARISVSE
                     REQIERVLETIQLTERRHELAGALSHGQKQWLEIGMLLMQEPRLLLVDEPVAGMTPQE
                     TERTAELLTSLAGKHSVIVVEHDMEFVRSIARKVTVLHEGGVLAEGSIDEVQNNPKVV
                     EVYLGV"
     gene            complement(2863..3993)
                     /gene="urtC"
                     /locus_tag="VNZ06_01315"
     CDS             complement(2863..3993)
                     /gene="urtC"
                     /locus_tag="VNZ06_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018928447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HWW19417.1"
                     /translation="MRTHNRLLAALQNDQGGRILLGLLLIVAVLAPVLNLLVPASSAL
                     HLSGYSVTLIGKYMCYAMLAIAVDLVWGYCGILSLGHGAFFALGGYAIGMYLMRQIGT
                     RGVYGDPILPDFMVFLNWKELPWFWHGFDMFWYAALMIVLVPGLLALVFGYLAFRSRV
                     SGVYLSIITQALTYALMLAFFRNDMGFGGNNGFTDFKQLLGFNLQSPGTRMALFLCTV
                     LALGGSYLMCRYIVASRAGRVIAAVRDAESRARFIGYRVESYKLWLFVFSAVLAGIAG
                     ALYVPQIGIINPGEFSPAQSIEIVIWVAIGGRGTLYGAVVGAVLVNYAKTYFTAALPS
                     FWLYALGALFVLVPLFLPRGVVGAIAGLRQRWSVRRPAVAGP"
     gene            complement(4002..>5658)
                     /gene="urtB"
                     /locus_tag="VNZ06_01320"
     CDS             complement(4002..>5658)
                     /gene="urtB"
                     /locus_tag="VNZ06_01320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HWW19418.1"
                     /translation="VSCRVERSGMRRLLRVCLLSLFCGLATIASAQDATQELAQLLKQ
                     LPTASFSEKEHLIQQLTGMHDARVRPVLTALLEGNLYNRTSDNRVFITQADGEQIRLT
                     DPLKGGSAGSASQDDLSRITVNNALRKALRVALAQFDLTSPNPGVRLQAVREMLRSVD
                     EETIGLLRQHLARESDGRVKREIRYALALSELDSGDTSVRLAAVEVLAGSLSNDVYNR
                     LNAMASPAADGNYSESDPRVRAAAQASIRSINRWRALFNAVQVLMFGLSQGSVLVLAA
                     IGLAITFGVMGVINMAHGELIMLGAYTTYGVQLLMPQHIGASILVAIPAAFLVSGLAG
                     VAIERSVVRYLYGRPLETLLATFGLSLILQQLVRDLFSANNRPVQTPDWLSGQLQVNE
                     ALSLTYNRLYIILFTFALFLLLLAVLKRTRLGLEVRAVSQNRAMAKAMGIRTEWVDAL
                     TFGLGSGIAGVAGVALSQLTNVGPNLGQSYIVDSFMVVVFGGVGNLWGTELGGNALGI
                     VNKLLEPFSGAVLAKIFVLVFLILFIQRRPRGLFPQKGRAAES"
BASE COUNT         1132 a         1882 c         1664 g          980 t
ORIGIN      
        1 ggtcgaattt cagggctgcg ttggtctcgt agaaatggta gtgcgaaccc acctggatcg
       61 gacgatcacc ggtgttcgac acggtcagcc gtcgcgtctc gcgctggtcg ttcagcgtga
      121 gctctccgtc gccaacaatc acttcaccgg ggatcatgct cacctcatgg gattggatga
      181 tgcaccgtca ccagcttggt gccatccggg aacgtcgcct cgacctgtac ctcggcaatc
      241 atctccggta caccttccat cacgtcactg cgcccgagca acgtggtgcc ataccccatc
      301 agctcggcga cggatcgtcc atcgcgcgcg ccttcaagga tcgcagcgga aatgaacgcc
      361 accgcttccg gatagttgag tcgcaggccg cgcgctttgc gacgttccgc cagcagagcc
      421 gcggtgaaca gcagcagctt gtccttctca cgaggcgaaa gttccatgac gtgctctctt
      481 caggtcttcc agatgcgcgg cgcgaccgct gcgcgtccca gcagtgtcat gcgcagcccg
      541 ctccacagat cgacgaaggc ctgtttcaat cgatctgctc gcgccgcata ggctcgacag
      601 catagcgttt tgtctatcag ggtacaggcc agcagcaggt ccgcatcctg gttccacgag
      661 cgcgcgatat cgcgggcctg ttcgaggtgc gccgcgcagg caggaaacgc caacagcgtt
      721 ccagcgctca ctcgccccgc caacccccaa cgtgcccgcg tcgcggtggc gtcgatcgtc
      781 acgcgctcga gcacgagcgc agcgtcgtca tgccacaact cgaggcattg tcggatacga
      841 ccttttccga gatcgagctg gctggcgggc aggccgaagc atgcgatctc ccagccgaaa
      901 aagtggcagt cgccctggag ccgtaccacg gtcgccagcg tcgcatccgc gttggggtag
      961 cagatattct cctgcggtag ccactccagt gtgccgcctt cgacgcgtaa cacttgggta
     1021 agcctcgcct ggcacgcgcc atagcgccga tagaacttgc cagccgcggg ggtggtcagc
     1081 agtgccagcg ccccggggcc gacctccacc tgcaacgaca gctcgtcgcc gctgaccact
     1141 ccgcccggtg gatgaatcac atagacgtga cacaccggac catcgggata gaacgctcgc
     1201 tgcaccatca ggggtccgcg gtgcaggcgg tgcgccagcc gggtgcgcga ctcgtccgcc
     1261 gcgaaccgca gctcgagcag gccctgccag ccggtcgcgg cctcttcctt cagcgcagtc
     1321 atacggtcag gtgggtgcgt accagctcat cggtcagagt ctctatcttg ccagagacca
     1381 cgagccgacc cttatcgagg atccgaagat cgctggccac ccggcgcgcg aatggcagct
     1441 tctgctccac caacagcacg gtgaggtgcg cctcacggtt gagacgctga atcacattgc
     1501 caatctcatg cacgatattt ggctggatgc cttcggtcgg ctcgtccagg atcagcagct
     1561 taggatcgag taccagcgcc cgcgcaatcg ccagctgctg ttgctgacct ccggacaggt
     1621 ccccgccccg ccggcgcagc atctgcttca aaaccgggaa catcgtgaag agctgctcgg
     1681 gaatctcgcg caaccgggtg cggcgcgctg ccagcccggt ttgtaggttc tcctcgacgg
     1741 tcagctgcga gaatatctcc cgaccctgcg gcacatagcc gatgcccatc ggcgcccgtg
     1801 cctccgccgg cagcgcgcgc agatcctgtc ccgcgtacag aatctgaccc gaggaggcag
     1861 gcagcaatcc catgatgcac cggagcagcg tggtcttgcc catgccgtta cggcccatca
     1921 ggcaggtgca ggaacccgtg ggcacatcaa gcgtcacatc ccacagcgta tgactgccgc
     1981 cgtaaaactg attgaggcct gtgatctcca gcatcttcta taccccgagg tagacctcga
     2041 ccaccttggg attgttctgc acctcgtcga tgctgccttc ggccagcacg ccgccctcgt
     2101 gtaacacagt caccttgcgt gcgatcgagc gcacgaactc catgtcgtgt tcgaccacaa
     2161 tgacggagtg cttgcccgcc agcgaggtca acagctcggc ggtacgctcg gtctcctgtg
     2221 gcgtcattcc cgccaccggc tcatcgacca gcagcagacg tggctcctgc atcagcagca
     2281 tgccgatctc cagccactgc ttctgcccgt gcgacagcgc tccggccagt tcgtggcgcc
     2341 gctcggtcag ctggatggtt tccaggaccc gttcaatctg ctcccgctct gataccgaga
     2401 tgcgtgcata cagccacttc cataccgact tgtcgcccgc catcgccagt tcgagattct
     2461 cgaatacggt gtgatgttcg aacacggtcg gcttctgaaa ctttcgcccg atgccggcct
     2521 gagcgatttc cggctcggtg agcgagagca ggtcgatcgt ctgaccaaac cacgccgatc
     2581 cggtatcggg gcgggtctta ccggtgatca catccatcat cgtagtcttg cccgcaccat
     2641 tcggccctat gatgcaacga agctcgccga cctcgatgta caacgtcagc gcgttgaggg
     2701 cgcggaatcc gtcaaagctc acagtgatgt cgtccaggta caggatgatg ccgtgtgagg
     2761 catccggtcc ggcttcgatg cggttggcct cgagcagctc accgacacgc gcaggcatgg
     2821 cgctgcgcga acgacccgag aaatggttca ggacctgccg gatcatggac cggccaccgc
     2881 ggggcgccgc accgaccaac gctggcgcag gcccgcgatc gctccgacca cgccgcgcgg
     2941 caggaacagg ggtaccagta cgaacagcgc gcccagggca tagagccaga agctcggcag
     3001 tgcggccgta aagtacgtct tggcgtagtt caccagcacc gcccccacca ccgcaccgta
     3061 gagtgtaccg cgccctccga tcgcgaccca gatcacgatc tcgatcgact gcgccggcga
     3121 gaactcgccc ggattgatga tgccgatctg cggcacgtaa agcgcgccgg caatacctgc
     3181 aagcaccgcc gagaagacga acaaccacag cttgtacgat tccacgcgat agccgatgaa
     3241 acgcgcacgg ctttcggcgt cgcgcaccgc cgcgatcaca cgtccggcac gtgaggcgac
     3301 gatgtagcgg cacatcaggt agctgccgcc cagtgccagc accgtgcaca ggaacagcgc
     3361 catgcgagta cccggtgatt gcaggttgaa gcccagcagt tgcttgaaat cggtgaagcc
     3421 gttgttgcct ccgaacccca tgtcattgcg gaagaacgcc agcatcagcg cataggtaag
     3481 cgcctgagtg atgatcgaca gatatacgcc cgacacgcgc gagcggaacg ccaggtagcc
     3541 gaataccaac gccaataacc cgggcaccag tacgatcatc agcgcggcgt accagaacat
     3601 gtcgaagcca tgccagaacc agggaagctc cttccagttc aggaacacca tgaagtcggg
     3661 caggatcgga tcgccatata cgccgcgcgt tccgatctgt cgcatcagat acatgccgat
     3721 tgcataaccg ccgagcgcga agaacgcgcc atgcccgaga ctgagaatcc cgcaatagcc
     3781 ccagaccaga tccaccgcga tcgcgagcat tgcatagcac atgtatttgc cgatcagcgt
     3841 cacgctgtaa ccggacaggt gcagcgccga agaggccggc accagcagat tgaggaccgg
     3901 cgccagtacg gcgacgatca gcagcaggcc gagaaggatt cggccgccct gatcgttctg
     3961 gagcgccgcc aggaggcgat tgtgggtccg cacgctacac gctagctctc ggccgcgcgg
     4021 cccttctgag ggaacagtcc gcggggccga cgctggatga acagaatcag gaacaccagc
     4081 acgaagatct tggccagcac cgcacccgaa aagggttcga gcaacttgtt gacgatcccg
     4141 agcgcattgc caccaagttc ggtgccccac agattgccca cacccccgaa taccaccacc
     4201 atgaatgagt caacgatgta gctctgcccg aggttgggac caacgttggt gagctggctg
     4261 agtgcaacgc cggccacacc ggcgatacct gaacccagac cgaatgtgag cgcatcgacc
     4321 cattcggtgc ggatgcccat cgccttggcc atcgccctgt tctgcgacac ggcccgcacc
     4381 tcgagtccca ggcgtgttcg cttcaatacc gcgagcagca gcaggaacaa tgcgaacgtg
     4441 aacagaatga tgtagaggcg gttataggtg agcgagagcg cctcattgac ctgcagctga
     4501 cccgacagcc agtcgggcgt ctgtaccggg cgattgttgg ctgagaatag atcccgcact
     4561 agttgttgca ggattagact tagaccgaac gtagccagca acgtctccag cggccgcccg
     4621 tacagataac gcaccacgct gcgttcgatc gcaacaccgg ctaatccgga aaccaggaat
     4681 gcggcgggaa tcgccaccag gatcgacgcc ccgatgtgct gcggcatcaa cagctgcacc
     4741 ccgtacgtcg tataggcgcc cagcatgatc agctcaccgt gtgccatgtt gatgacgccc
     4801 atcacgccga acgtgatggc cagcccaatg gcggccagca ccagcaccga cccctggctc
     4861 agaccaaaca tcagcacctg taccgcattg aatagtgccc gccagcgatt gatcgagcgg
     4921 atcgatgcct gggcggcggc gcgcacgcgc ggatccgact cactgtagtt gccgtcggca
     4981 gcggggctcg ccatggcgtt cagccggttg tagacgtcgt tgctgaggct gcccgcgagc
     5041 acctcgacgg cggcgagccg cacgctcgtg tcgccgctgt ccagttccga caatgccagg
     5101 gcatatcgga tttcacgctt cacgcgacca tcgctctccc gcgccagatg ctgtcgcaac
     5161 agcccgatgg tctcctcatc caccgagcgt agcatctcgc gcacggcttg cagccgcacg
     5221 ccgggattgg ggctggtgag atcgaactgc gccagggcca cacgcagcgc cttgcgcaga
     5281 gcgttgttga cggtaatgcg gctcagatca tcctgcgagg cgctgccagc ggatccgcct
     5341 ttcagcggat ccgtcagcct gatctgctcg ccatcggctt gcgtgatgaa tacgcggttg
     5401 tcactggttc gattgtagag attgccctcg agcagggccg tgagaaccgg gcgcacgcgc
     5461 gcgtcgtgca tgccggtcaa ctgctggatg agatgctcct tctcgctgaa gctggcggtg
     5521 gggagctgct tgagcagctg cgcgagttcc tgcgtggcat cctgagcgga cgcgatcgtg
     5581 gccagtccgc agaacagact caacagacac actcgcagca atcgcctcat accactcctc
     5641 tcgacgcgac acgagaca
//
LOCUS       DATMRM010000049        24623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_649501, whole genome shotgun sequence.
ACCESSION   DATMRM010000049 DATMRM010000000
VERSION     DATMRM010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 24623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24623
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649501"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="VNZ06_01325"
     CDS             complement(<1..1591)
                     /locus_tag="VNZ06_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19419.1"
                     /translation="MRRISFGVLLIALSTLVLEIMLTRTFDVTLAPNISYFVVTLAVF
                     SFGLAGIYATLRPIPVDQDIRHILTSRSISYAAMMLILIPLINALPLDYTHLGHHPFE
                     IVGSYLMLYLALLVPFFLAGYVLIAVFSKYAASIQRLYFWDLIGAGIGTLLVIPLVAK
                     IGPGGLIVCAAALALLAAALFSANRALTGIAVVAALVILSIPFIRAPKYIDFEEKMDK
                     RGVLADLRAGLGEFERWDPIAKIDVIDQKWTPEKAQPWWPFGDRKIIQYDGGNQSSYF
                     YPFDGDLKAFRVSLDHDKSHIKAHFWQIGVLASHYLKRDTDHSVLVIGSAGGQETKAA
                     LVYGATHVDSIELVPTVVELAKGRYSHYIGDIFLNPAVQAQPGEGRSFLRHSNRTYDI
                     IQIYSNYTNSSVAQGTGALSPEYLQTAEAYEEYFSHLTANGILHINHHSYPHLITTAA
                     LAWKQLGRSDDFQRHVVVYISDAEPFLPTILIKMQPWTQAQIDELSAFLAPAELNQYN
                     RQHMVENPVDFSKSFLSADFYS"
     gene            complement(1756..3387)
                     /locus_tag="VNZ06_01330"
     CDS             complement(1756..3387)
                     /locus_tag="VNZ06_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19420.1"
                     /translation="MELRRDQHFYLGEARAALAPAIRWFSRPASPTSAFLVGVVIAGA
                     YVLALCACGALMFQDYPNHLARAVILADLWFHHGEHFGSQFQFQSMLYPYIAADWLLT
                     PLVELLGQQGATFVWTALALLSLPLAMLHYLHATRVRVEARPFVFLVSLYLSTNTFFF
                     LGFVNFSFALALTILVTGLAQQLREHRSPTRYALYTVMIALGFLMHLAFVVFAAVVVG
                     TTGLVRLYRRQSTLIREIWLLLPIVVTLAWYVLSVQIYPATHDTVSARFIWPGIFGKI
                     RQLDWPFQRFDAAIDLGFAVAFAVLCAAITLRRVDRRKLSRRASIEALALIAGCGLLY
                     VLLPSSYGDPTYIDMRALPLVALAGLLWCVYLSLGAPGVSPLTSRAVLTGAGLLLAAN
                     LYYLGAELAPQAAWLRQYRSLLTSVPQGSKVLPIYTGTHQLTRWQYLHVASYVVTDRK
                     GIEPYLFSGDQGQPMKYFRYRNHPYAPHNTWYSYTPQSGVPAVVDWPRIACSYDYLLV
                     MKPFNLARIELSNTTIAENSSAALLTADTRGCPTR"
     gene            complement(3333..4466)
                     /locus_tag="VNZ06_01335"
     CDS             complement(3333..4466)
                     /locus_tag="VNZ06_01335"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWW19421.1"
                     /translation="MDDVARDVYAQLVAGPELAVIVPTFKEVKNVPELVERLRQSLVG
                     ISWEVIFVDDDSPDGTSDCVREIARLDWRVRCLQRIDRRGLSSATIEGMHASSSPFLA
                     VMDGDMQHDEQLLPDMLHALKSGNLDVVVGSRYVEGGTLGDFAARRVGMSKFATRLSR
                     LVVPPALTDPMSGFFLIRREVFTAANRKMSAIGFKVLVDLFASSPRPLRFLELPYEFR
                     TRRAGESKLDSAVVWDFGLLLIDKTVGHIVPARFFAFTVVGLFGVVVHLSVLAALLNL
                     LHWQFSIGQALATGVAMIVNFSINNAVTYSDKKLRGWRWFGGLLSFAAVCGIGALANV
                     GVASYLFRWRAAWIPAALAGILVSAVWNYVATSIFTWAKPGRR"
     gene            complement(4695..6260)
                     /locus_tag="VNZ06_01340"
     CDS             complement(4695..6260)
                     /locus_tag="VNZ06_01340"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWW19422.1"
                     /translation="MAADRYELCIIGAGIAGALLAVTAARQGRKVLLVEAGNRFPLEG
                     RRQLLRRYDTLGGLYVPWQHPGRDRYVDSSGQSIGYPYELERYRAKGVGGSTLHWGGR
                     AQRLMPSDFRTASTYGLGTDWPLSYDELEPYYCAAEWEIGVSGSPHTLQAPRSRDFPM
                     PGFPFSPDERMWLPIAQKMDIELYSAPFAINSMPYAGRTSCKAYSACNICPSGARYCA
                     DIHVAEAEKTGNCVLLTETVARRIDLNASGTVTRIHATTLDGRDLEVQADRYVIAAHG
                     VESARLLLLSKCGNHSDQVGRNFMEHPFIKAAGYVPTRQFYPDVVGFERLESMSFYEG
                     KDRQKRGAFKLEFEFSYDPLRDMEFDHLWGDALARHDQKLFGHWLGINVETEMQGNAD
                     SRVTLDSKAKDMFGDPVPHLRIAFCDTDIRTQRRALQVATELLQAAGAQDIRPIGPNG
                     LSFPAHHMGTCRMSKDPDAGVVDRDCRVHGTSNLYVVGSSVYPTGGGCNPTLTIAALS
                     LRLADHLLQSNAS"
     gene            complement(6260..6904)
                     /locus_tag="VNZ06_01345"
     CDS             complement(6260..6904)
                     /locus_tag="VNZ06_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19423.1"
                     /translation="MHASSRFPSVICWHDPLHMASHGHPSRRKFLIVTAIGGVIAALA
                     LAPKGRLRLLVPARLPDVSSQSALTTATLFIGALFAHELTSEDSADLLQRLRETIELE
                     SARARDYQVLAQYLDQAAHSSGADSFAAATAALRNHILGELMRVNPGTHFAHLLSRLS
                     AGRREYYRMRASTVPALAWLYRRSSIPWRARGYRRPPGVPDDWRDYLTAGSRQS"
     gene            complement(6950..8059)
                     /locus_tag="VNZ06_01350"
     CDS             complement(6950..8059)
                     /locus_tag="VNZ06_01350"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWW19424.1"
                     /translation="MLRHFDQKDGGVKVYTHSILPRLLSLPSRHHFVLIYQDPKRLGT
                     YPQYPNCEEIAVRIPGTVLWDQVAVPWVVRTRHLDMVFNPKITIPFLSGAKKAYVVHG
                     AEKFAIPEHFQTFDRWYFERMMPLYGQRADAVVAVTNCVKADVVRHLHVDPKKVFVIY
                     NGFDSALFHRKEDPAYLRKVAQRYQLPDRFILWAGQIESRKNLPRLLQAFAKLRLVIP
                     HHLVLAGAQRANYTQELRAIRELGLEDRILMPGWISHEDLAALYSLAELFVFPSLHEG
                     FGIPLVEAMACGCPIVAANTGSVPEVVDQAACLVDPLQPDEIAGGIQRVLADATLRAE
                     LSAKGLERAKIFSWDKTARQVLDMLDSLDELRHAA"
     gene            8296..9102
                     /locus_tag="VNZ06_01355"
     CDS             8296..9102
                     /locus_tag="VNZ06_01355"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWW19425.1"
                     /translation="MANDSNRDWQLYGEVDPYFGVISLDRFHKNNLTNAALSEFFSSG
                     EQHIQYVLDIVRTHLDPTFRPICALDFGCGVGRCTLPLARLCEQVTGVDVSQAMLEEG
                     QANARKHMLSNIKWLNSSDLSNLNGSFEFVHSFIVFQHIPPARGMPLLARLIDLLAPE
                     GIAAIQVLYHRDVSRLKQLVGRLRVSVPLMHNLANLYYGKPFNYPLMQKNVYDLSAVF
                     DLLQKKQCGSSWVTFAQSDELQSAMMFFRKRPDAMPYYEFYNAAKNAQSA"
     gene            complement(9080..9904)
                     /locus_tag="VNZ06_01360"
     CDS             complement(9080..9904)
                     /locus_tag="VNZ06_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWW19426.1"
                     /translation="MRLADVVLPPAQFGAMQRAKQIARGFTWRLTHPGRTHACPFCQR
                     EFGQFIPYVNRFPIAVTADIISGQSISFFKCPYCQSRDRERGLYFYIRKQLSLRGKRI
                     LHVAPERALHDLIAASGCLDYVCGDINPKAYYSTSRAIHRIDLLSIDFPDGSFDFAIC
                     NHILEHIADDRTAMSELFRVLKPQGSAIVQVPIGAKLETTYEDPRMRTADERSRAFGQ
                     DTHVRIYAEADYLERLRSVGFETRADSIPISPADFARYGIDPREKVYLALKPTERS"
     gene            10308..11708
                     /locus_tag="VNZ06_01365"
     CDS             10308..11708
                     /locus_tag="VNZ06_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19427.1"
                     /translation="MNWRKPLIRAALAFQNPHVLRELRLIRSIERATPDTVAAVQRAR
                     LTALLHFAWSETDYYRDLLDACGAVRDGRVNLDRFQDIPFLTKDLIRAHQSRMRPRSL
                     PKGRSAHINRSGGSTGEPLQFWQDNVYWAATIATRTYHFSLTGKDIGEREMKIWGSEQ
                     DLFKGTIGARAKFENWIYNRQFEQCWHLPEERILKIIHDINTWRPKMLWCYRDGIDAV
                     ARYVNQNRISMHSPAAIVLGGATVYPFMVQAIEKAFEAPAISAYGSREIGAAACQCLK
                     GQGHHIASHTHVIESIGSDDQPHYEREGDLAITPLLNYAMPFIRYRIGDRGVLTRDLC
                     SCGRSFPLLSALTGRTVEVFTNSKGEQVDPIYFIQLLSVIFNQGFVRKFQVVQEQDGG
                     ITINFVLASGVDLDATGSNFDDIRSKILLVMGRNCLVRFNAVDDIPLSASGKYPYIVS
                     RRSLKSAERTEELQPT"
     gene            11805..13274
                     /locus_tag="VNZ06_01370"
     CDS             11805..13274
                     /locus_tag="VNZ06_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19428.1"
                     /translation="MDNDLSNLDGSSRAKKRIGKMLDSMVLRQLGGRTATRTLNWRKP
                     LIRAAFALKNPGTLAELDLIQSIERSAPESIQAVQQARLEQLLHHAWSQTDYYREVLE
                     QCGVVQDGRINLDRFENIPFLTKDIIRTQGDRLCARKLPRGRKAYSNLSGGSTGTPIR
                     FMQDSGYWDVTIATRSYHFSFAGKELGEREMKIWGSERDILQGTLGLRASLENWLYNR
                     KFEQCFKLPEQQIRKIIRDINTWQPKLLWCYRDGIDAVARYATEHDIELHSPAAILLG
                     GATVYPFLAKQIERAFHCPVLSAYGSRELGAVACQCIRGQGHHIASQAHVLETINVAE
                     EPMMEQDGELVVTPLLNYAMPMIRYRVGDRGRLTRRLCSCGRNFPLLESLTGRMIEVL
                     INSKGEQVDPIYFMMLLSEVFANSPVRKSQVIQEKDRSITMNLALDKGATLNPSNPLL
                     VAARKKVAAVMGTDCRVRFEFVADIALTASGKFPYVLRR"
     gene            complement(13295..14797)
                     /locus_tag="VNZ06_01375"
     CDS             complement(13295..14797)
                     /locus_tag="VNZ06_01375"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWW19429.1"
                     /translation="MAQLASLDALNASEQYDVCIIGSGPAGTTLATTLARRGIRTLLL
                     ESGRGLRSWLTDAQLKGLARYEFTGDTDYPLTQTASRLLGGNSNFWTGRCERLHPSDF
                     EPHPYTPADNPWPIRYSDLAPYYEAAEQMLRVRGGPRTRFAPPRTGPLPLPGSPDITL
                     LTQLCAQFGVEVEDSATATPTKTWRFFNVQKEILPNFLSSGHGTLLTGATVTNLVAAQ
                     QRIVAADVKTLDGHGATARARIFVVCCGGFETPRLLQLSAAEAFPNGIGNAHDMVGRG
                     FNEHPNVGLSAHIPHNRLTLTPTNKIARTHQFYQTFRSEGLGAIVPVFRQSWLMPNHL
                     LRFQLSAIPTTLLSLFKRVVNAPIHFGSSIEMKISPANRVALSKTRTDAFGRPVAQLI
                     FHYSDEDRALIERARALLRGWLQQLRATDIEDIGITWSRHLQGACRMGDNPRSSVVDR
                     DLKVHEAPNLYLCGSEVFVTGGGMQPTLTIAALALRLADTLTQRLSRSGQ"
     gene            complement(14812..16071)
                     /locus_tag="VNZ06_01380"
     CDS             complement(14812..16071)
                     /locus_tag="VNZ06_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19430.1"
                     /translation="MPTGVLRSLFKSKSLRAASAFGASGAALAIGNLLLARVLSPEHL
                     ASFSLVFALVMIGVNVGPMGADVILTRNHYDTSSKLHLQVFLTSCAVGLLLILTAIWL
                     YPLSFALLAAVFITTVAGSVKVVATSYYRSQERFLAALALTASTNASILVAGVASMFV
                     ARSSALLPTTVMTVTMCSAALIGWYSVVSDRERVQQNTAQSYPWNESWHAANFIAASM
                     IMTSLERITVPKLLDLPTLATFAVLATLAGSPFTILYQGVSFTLVPALRNAPDARARR
                     RVFGRETAIIAASCVLAAFAAWWLTPLFLTYVLAGRYHIAPSLLSVAIISGILKVVGT
                     LPASAVNALGSASHLVKLSMAGWLTIAVSLVGAAIGSRWGLAGLVSGVAAGWLVRALV
                     IGWLAFPLFSKSAAPAMGSTRQSELAR"
     gene            complement(16071..18536)
                     /locus_tag="VNZ06_01385"
     CDS             complement(16071..18536)
                     /locus_tag="VNZ06_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19431.1"
                     /translation="MRRLSFGVFLTALSTLILELMLTRAFDVTLAPNISYFVVTLAVF
                     SFGLAGVYATLRPIAVDSEIRPMLSNRSFWYAAAILVLIPIINALPLDYTRLGRAPFS
                     TLSAFIVLYFVLLIPFALAGYILIAVFSKYAQSIQRLYFWDLIGAGVGSILVVPLISK
                     LGPGGLIICAAGLALIAAALFATSRAASISACVLAAVILAAPILRAPQYIDFKQHMDK
                     RGLLGALQSGMGEFVRWDPISKINVVREDWTPEKAQPWWPMGNRKAIQYDGGNQTSYF
                     FPFDGDLKALRARLDIDKSRVKEHFWQLGVLAAHYLKRDSNQSVLIIGSAGGQETKAA
                     LIYGAAHVDAVELVPTVVELATGSYSDYIGNIFHNSAVNVQAGEGRSFLRHSNRSYDI
                     IQIYSNFTSSSVAQGTGALAPDYLDTAEAYQEYFSHLSANGVLQVNHNTFPRLISTAA
                     LAWKRMGRTDLASHVTVFFSESEPMLPTLLIKMQPWTAAEIADLSAFLAPSELSDYYR
                     LHIVENPLDRTKSFLSADFYSGDFPASLAERMAANVTPRTDNRPYFGLTRKSIRELKP
                     DPSNYLDEGTAYALNSSLIKGIIPMDLIHLIVTGVASIIFVIVFVFVPLRFSKVGREE
                     RAAAVPLLVYFSCLGAGFIIIELVSIQKFIHLIGSPLYTYSTVIFTMLFSAGVGSVAS
                     ERLGLTTRQRWALPFTAILVIGLAFTAFYPSLARIALALPVTGRVLASAAMIFPLGFF
                     LGMPFPLGILAISNQPRGAIAWAWGMNGLFTVVGGFMSIVLSLLYGFNFAILLALGLY
                     LLAFIMFPRVRDMQPRTSSSGSN"
     gene            18816..19550
                     /locus_tag="VNZ06_01390"
     CDS             18816..19550
                     /locus_tag="VNZ06_01390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWW19432.1"
                     /translation="MPRRKELTATIEPFDSFWEAPDDIEKGYHSFWLFYKDNYQKYFP
                     SDKQAKILCVSCGPGYGVKLLADLGYQDVLGIDSFEDKIAFATAKKLNCRMGYAFEFL
                     QDAPDESYDLIWLEQEVNHLTRSEILEFLALCLRKLRRGGRLIAFALNGANPITGAEA
                     LAQNFDHFNTFTEYTLRQVLNHTGFEKIEVFDLNLYVFYKNPFNYVGMAASAFLSMLF
                     RACFLLYGKKNRLFGKKIAASCLRPA"
     gene            19805..21541
                     /locus_tag="VNZ06_01395"
     CDS             19805..21541
                     /locus_tag="VNZ06_01395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HWW19433.1"
                     /translation="MPLEPSLGDTPRTSSLNRLANETFDVLVIGGGIGAACTAWDAAL
                     RGLRVALVERLDFGSGASAHSFKVLHGGIRYLQHLDIVRLRESCRERGAFLRIAPHLT
                     RPLPFAVPTFGYAMQSRWVFGAALATLETLTCDRNRATRDPLRRVPKSYLLTRDELLR
                     RFENVDGDGLTGAGVFFDGQILNPPRAVLAVIRSAASHGAAVANYCEAQEMVVRDGRV
                     VAMRARDLIRGEVFDIRARVIINATGPFAPSFVRRLGAGVQLNVPLSRDMAFVVRRVL
                     DPEMAVGVQTRYRDPDAMLSRGNRHLFMAPWRGRYTLIGVNSRVYTEDAYDLHVTEGE
                     IEGFLQEINEASPPLAVRRDEVTVVNAGLLPFGENSPDQKDLSFGKRSQIIDHASTGG
                     PEGLVSAMSIRWTMGRLLGERVVDLAQQKLGIKAVPCSTTTTRVWGGDIESVEGYARE
                     MRRLLPAAVSDAQATRMFQHYGTKWSEVLAGDDREVIQGDMIPATDYFGAEVRYAVRN
                     EMASTLSDVVMRRLGLGSAERPADETLAACASIAGDELGWSLARREREIQSVRDSFPF
                     ASPRSQYPRFDS"
     gene            21538..22770
                     /locus_tag="VNZ06_01400"
     CDS             21538..22770
                     /locus_tag="VNZ06_01400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW19434.1"
                     /translation="MSAQFRIICPTYWYPGSATDMRAIYVHDITRHLVQRGHRVCVVT
                     PAGTGAPLRDTLDGVEIHRFPLAVPEDLAYGKVAQSKVGLVSRMVRMQVMVQYLWRQY
                     RAMLDCARDFKPDVVHGHWAIPTGPALVYGSQKLRIPSVITMHGGDVYVNKAEGYDFP
                     TRPYVKPVLHWTLTRASALTAISDDCVHHALRAGASMEKIHLIMNGADLRRFSPSGET
                     PDRPYGNKMVFACRQLFPRKGIRYLIQAVARLRPQHPDIRLVLAGDGFERAELEALAD
                     KLGVGDITTFLGWVVNSDLPKYFRACAVSVIPSLEEGFGIPAAEAMGCEVPVVATDAG
                     GLPEVVANGETGFVVPKADANALAEAIGKLLTDSALAHRMGKAGRQRALERFDWMRSA
                     ERFEELYTSLLSPAGVAS"
     gene            22767..23615
                     /locus_tag="VNZ06_01405"
     CDS             22767..23615
                     /locus_tag="VNZ06_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWW19435.1"
                     /translation="MNERVFSFRWDIDHRACITDGLPKILDVCDELGVKNTFFVNMGR
                     STNLREWLGKGLRGSRAKLNDMEAVHLIKKIGWARFGLETLLSRPVGRSLIKPLEELQ
                     NRGHELGLHGGSDHVIWSRRFAQLSAEEIEADVARTLETYTRYFGKPAGFTSPGFKHD
                     ERTSRLVEKLGFKYDGDALGGEPHHPTFDGKPGKHWTIPVTLCGPRTIPLIEWHGARG
                     VCEDEIVADVMRRMADERWVVLYGHPCYEGVRAGLLRRILRAVQEAGFKFLTHLQMAE
                     RLYAGT"
     gene            23602..>24623
                     /gene="wecB"
                     /locus_tag="VNZ06_01410"
     CDS             23602..>24623
                     /gene="wecB"
                     /locus_tag="VNZ06_01410"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HWW19436.1"
                     /translation="MPVPNLPLRVLSVFGTRPEAIKMAPVVRALESQSQHFKSLICVT
                     GQHREMLDQIMSVFKLSADFDLDVMRAGQTPAEVMSRILAAMPGILNQAKPDLILVQG
                     DTTTTAAVALAAFLQRIPVGHVEAGLRTDDISQPFPEEMNRRVASLVTTLHFAPTMRA
                     QNALLQERVPSERVIVTGNTVIDALLQARRPDYRFVNSSLATLDVTRRVVLVTLHRRE
                     SFGAPMLSVCRALNRVAQGHPDLIFVLPVHRNPSVREVVVPALQGCPNFMLIDPLDYL
                     DFIHVMARSRVIVTDSGGVQEEAPTLNVPVLVAREVTERPEGVEVGAARLVGTDENRI
                     VQMLEEL"
BASE COUNT         5096 a         7156 c         7628 g         4743 t
ORIGIN      
        1 ccgagtagaa gtcggccgac aggaagctct tgctgaaatc gaccgggttt tcgaccatgt
       61 gctgacggtt gtactgattg agctcggctg gggcaagaaa cgcactcaac tcatcgatct
      121 gcgcctgcgt ccacggctgc atcttgatga ggatcgtcgg cagaaacggc tcggcgtccg
      181 atatgtagac caccacatgg cgctgaaagt cgtccgacct gcccagttgc ttccacgcca
      241 aggccgcagt cgtgatcaga tgcgggtagc tgtggtggtt gatgtggagg atgccgttag
      301 cggtcagatg cgagaagtat tcttcatagg cttccgccgt ctgcagatac tcgggcgata
      361 gcgctccggt gccctgcgcg acgctggagt tggtgtagtt gctgtagatt tgaatgatgt
      421 cgtaggtgcg gttgctgtgg cgcaaaaaac tgcggccctc accaggctgc gcctgtaccg
      481 ccggattcaa aaagatgtca ccgatatagt gtgagtagcg tccctttgcc aactcaacga
      541 ccgtcggcac caactcgatc gaatcgacgt gggtcgcgcc gtagaccagt gccgccttgg
      601 tttcctgtcc accggcgctg ccgatgacca gaacactgtg gtcggtgtca cgcttcaggt
      661 agtgcgaggc tagtacgccg atctgccaga agtgcgcctt gatgtgcgat ttgtcgtgat
      721 ccagggagac tcgaaatgct tttagatcgc cgtcgaatgg atagaagtag ctcgactgat
      781 tcccgccgtc gtactggatg atcttgcggt caccgaacgg ccaccagggt tgcgccttct
      841 ccggtgtcca cttctgatcg atcacgtcga tcttggcgat ggggtcccag cgctcaaatt
      901 cacccaagcc cgcccgcaga tccgccagca cgccgcgctt gtccatcttc tcttcaaagt
      961 cgatgtactt cggcgcgcgt atgaacggta tcgacaggat cacgagggcc gcgaccaccg
     1021 cgatgccggt cagtgcgcgg ttggccgaga acaaggctgc ggccagcaaa gccagcgcgg
     1081 ccgcgcacac gatcagcccg cccggtccga tcttcgcgac cagcggaatg accagcagtg
     1141 tgccgatgcc ggcgccgatc agatcccaga aatacagccg ttgaatgctg gcagcgtact
     1201 tcgagaatac agcgatcagt acgtagccag ccaggaagaa cggcaccagc agtgccaggt
     1261 acagcatcag atagctgccg acgatctcga acggatgatg gccgaggtgg gtatagtcca
     1321 gcggcagtgc attgatgagc gggatcagga tgagcatcat cgcggcgtag ctgatgctgc
     1381 gcgacgtgag gatatggcgt atgtcctgat cgaccggtat cggccgcagc gtggcgtaga
     1441 tgccggccag accgaaagag aataccgcca gggtcacgac aaaatacgag atgtttggcg
     1501 ccagcgtgac atcgaatgtc cgagtcagca tgatttccag cactagcgtg gacagggcaa
     1561 tcaacagaac gccaaacgat attcgtcgca tctttaatcc tctaggcagc tggcgcccgg
     1621 cccctacgag tccgagcgca atcaaaagct tacttcatga agaatacatt aagtcgtgcc
     1681 cggaacccgg atacccggtt cacgaaacca agcttgacat cgaatgcgga attagcgctg
     1741 cgggcgagcg gcgcgctagc gagtcgggca gccgcgggtg tccgcagtca gcagtgcagc
     1801 gctcgaattc tcagctatgg tcgtgttgga cagttcgatg cgcgctaggt tgaacggctt
     1861 cattaccagt aggtagtcat agctgcaggc gatgcgaggc cagtcgacaa ccgcaggcac
     1921 tccggactgg ggcgtgtagg agtaccaggt gttgtgcggc gcgtacgggt ggttgcgata
     1981 gcgaaaatac ttcatcggct gcccctgatc gcccgagaac aggtaaggct caataccctt
     2041 gcggtccgtg acgacgtacg aagcgacgtg cagatactgc cagcgagtca actggtgcgt
     2101 gccggtgtag atcggcagga ccttcgagcc ctgtggcacc gaggtcagca atgagcggta
     2161 ttgacgaagc cacgcggcct gcggggcgag ttccgcgccc agatagtaca ggttagctgc
     2221 cagcaggagc cctgctccgg tgagcaccgc gcgcgaggtc aaagggctga ccccaggtgc
     2281 gcccagggac aggtacacac accacagtaa gccggctagg gccaccagcg gcagtgcccg
     2341 catgtcgatg taggtagggt ccccatacga tgacggcagc agcacgtaca gcagaccgca
     2401 tcctgcaatc agcgccagcg cctcaatcga ggcgcggcga gatagttttc tgcgatcgac
     2461 ccgccggagc gtgatcgccg cgcacagtac ggcgaaggcc acggcgaacc ccagatcgat
     2521 cgcggcgtcg aagcgctgga acggccagtc cagctgtcgg attttgccga agatcccggg
     2581 ccagatgaag cgcgcggaga cggtatcgtg ggtggccgga tagatctgaa cgctcaacac
     2641 gtaccacgcc aacgtcacaa cgatcggcag cagcagccag atttcgcgga tcagcgtgct
     2701 ctggcgacgg tataggcgca ccagtccggt agtacccacc accacggcgg cgaacacgac
     2761 gaacgccaga tgcatcagga agcccaatgc gatcattacg gtatacagcg cgtagcgtgt
     2821 cggggatcga tgttcccgca gttgctgggc cagcccggtg accagtatcg tcaacgcgag
     2881 agcgaaactg aagttgacga atccaaggaa gaagaaggtg ttggtggaca gatagagtga
     2941 cacgaggaac acaaagggtc gcgcttcgac acgcacccgt gtcgcatgaa ggtagtgcag
     3001 cattgcgagc ggcagcgaca gcaaggccag tgccgtccag acaaaggtcg cgccctgttg
     3061 tccgagtaac tcgaccagcg gtgtgagcag ccagtccgcc gcgatgtatg gatagagcat
     3121 gctctgaaac tggaactggc tgccgaagtg ctcgccatga tggaaccaca gatcagccag
     3181 aatcacggcg cgggccagat gattcggata gtcttggaac atcagagcgc cgcaggcgca
     3241 caacgccagg acgtaggcgc cagcgatcac gacccccaca agaaaggcag aggtcgggga
     3301 ggccggtcgc gagaaccagc ggatcgctgg agctagcgcc gcccgggctt cgcccaggta
     3361 aaaatgctgg tcgcgacgta gttccatact gcactcacga gaatgccggc gagcgcggcg
     3421 gggatccacg ccgcgcgcca gcgaaataaa taggaggcga cgccgacatt cgcgagcgcc
     3481 ccgatgccgc acactgcggc gaatgacagc aggccgccga accatcgcca gccgcgcaat
     3541 ttcttatcgc tgtaggtcac ggcgttgttg atcgagaagt tcacgatcat cgcgacaccc
     3601 gtggcgagcg cctggccgat tgagaattgc caatgcagca agttcagcag cgccgcgagc
     3661 accgacaggt gcaccacgac gccgaacaga ccaacgaccg taaaggcgaa aaatcttgcc
     3721 ggaacgatgt ggccgacggt cttatctatc agcagcaggc caaagtccca gaccacggcg
     3781 gagtcgagtt tgctctcgcc cgctcggcgc gtacgaaatt cgtagggcag ctccagaaac
     3841 cgcagcggac gcggtgagga cgcaaacaga tccaccagga ctttgaaccc gatggcagac
     3901 atcttgcgat tggccgcggt gaacacctcg cgtcgtatca ggaagaaccc gctcatggga
     3961 tccgtcagcg ccggtggtac caccaggcgg ctgagacgtg tcgcgaactt gctcatgccg
     4021 acgcggcgag ctgcgaagtc gccgagcgtg ccgccttcaa catagcggct gccaacgacc
     4081 acatccaggt tgccgctctt cagcgcgtgc agcatgtcgg gcagcagttg ttcgtcgtgc
     4141 tgcatgtcgc cgtccattac cgccaggaaa ggcgaggagc ttgcatgcat gccttcgatt
     4201 gtggctgagg acaggccgcg acgatcgatg cgctgcagac agcgcacgcg ccagtcgagg
     4261 cgcgcgattt cgcgcacgca gtcgctcgtg ccatcgggcg aatcatcatc gacgaagatg
     4321 acctcccagg agattcccac caggctctgt cgcagtcgct cgaccagctc ggggacgttc
     4381 ttgacctctt tgaaggtcgg cacgatgact gcgagctcgg gtccggccac cagctgggcg
     4441 tagacgtcgc gcgccacatc atccatagat tcgctgacct gtcgtcgagc gtcgtgttga
     4501 tcacaactgc ttcgtgcttt cattctatca gtgtgacaga gcgctgatgt gagtctctgt
     4561 cagcttggcg gcgcccgcag gtgcgtcggt gcgacataaa gagtgcgcgg aaagcttgag
     4621 gcaagctacg ggcactgagc gatccagcga tgagatcgac ttctcggatc aagaatcgtt
     4681 cacagattca agagttagct tgcgttgctc tgcagcagat gatcggccaa tcgcagactc
     4741 aaggctgcga tggtcagagt cggattgcag ccgccgccgg tgggatagac gctgctgcct
     4801 acgacataaa gattcgatgt gccatgcacg cggcaatcgc gatccacaac gcctgcgtcc
     4861 ggatccttgc tcatgcggca ggttcccatg tggtgggcgg ggaacgatag gccattcgga
     4921 ccaatcggcc gaatgtcttg cgcgccggcg gcctgcagca attcggtagc gacctggagc
     4981 gcacgtcgct gcgtgcggat gtcggtgtcg caaaatgcga tacgcaaatg cggcaccggg
     5041 tcgccgaaca tgtcctttgc cttggagtcc aatgtgacgc gactgtcggc attgccctgc
     5101 atctcggttt cgacattgat ccccaaccag tgtccgaaca gcttctggtc gtggcgcgcg
     5161 agcgcgtcgc cccacaggtg atcgaattcc atgtcgcgta acgggtcata ggagaactcg
     5221 aattcgagtt tgaacgcgcc tcttttctgc cggtccttgc cctcgtagaa agacatgctc
     5281 tccaggcgct cgaaaccgac cacgtccgga tagaactgtc gtgtcggcac atagccggcc
     5341 gctttgatga aggggtgctc catgaagttg cgacccacct ggtcggagtg attgccacac
     5401 ttggacagca gcagcaggcg cgcggattcc actccatggg cggcgatgac gtaccggtcg
     5461 gcctgcactt ccagatcgcg gccgtcgagc gtcgtggcgt gaatcctggt caccgtgccg
     5521 gacgcgttga ggtcgatgcg tctggcaacc gtctcggtca gcagcacgca gttgccggtc
     5581 ttctcggctt cagccacatg gatgtccgcg cagtagcgcg cgccgctggg gcagatgttg
     5641 caggccgaat aggctttgca ggaggtgcgg cctgcgtacg gcatcgaatt gatcgcaaat
     5701 ggcgctgagt aaagctcgat atccatcttc tgcgcgatcg gcagccacat acgctcgtcc
     5761 ggggagaatg gaaagccggg catcggaaag tcccgactgc gcggtgcctg caatgtgtgc
     5821 ggactgccgg aaactccgat ctcccattcc gcagcgcagt agtacggctc gagttcgtca
     5881 tagctgagtg gccaatctgt tcccaaacca taagtggatg ccgtgcggaa gtcggagggc
     5941 atcaggcgct gcgcgcgccc gccccaatgc aaggtggatc cgccgacgcc tttggcgcgg
     6001 tagcgttcca gttcataggg gtatccgatc gactggccgg agctgtcgac gtagcggtcg
     6061 cgtcccgggt gttgccaagg gacgtacagt ccgccgagcg tgtcgtatcg ccgcagcagc
     6121 tgcctgcgcc cttcgagcgg aaagcgattg cctgcttcca ccaggagcac cttgcggccc
     6181 tgtcgggcgg ccgtgaccgc cagcagcgct ccagcaattc cggcaccgat gatacagagc
     6241 tcgtagcggt ccgcagccat caggactgcc ggctgccggc cgtaaggtag tcgcgccagt
     6301 catcgggaac tccgggcggc cgccgatagc ctcgagcgcg ccacggaata ctcgagcgac
     6361 gatagagcca ggcgagcgcg ggcacggtgc tcgcgcgcat acgatagtac tcacggcgcc
     6421 cggcggatag ccgtgagagc aggtgggcga agtgcgtgcc aggattgacc cgcatcagct
     6481 cgccaagaat gtgattgcgc agcgcggcgg tcgctgcggc aaaactatcc gcacccgagc
     6541 tgtgcgcggc ctggtcgaga tactgcgcta acacctgata gtcgcgagct cgcgcggatt
     6601 cgagctcaat ggtctcacgc agtcgctgca gaaggtcggc gctatcctcg ctggtaagct
     6661 cgtgagcgaa caacgcaccg atgaatagcg tcgcggtcgt gagggcgctc tggctcgaga
     6721 cgtccggcaa tctggccggc accagcagcc gcaatctgcc ttttggagcg agcgcaagcg
     6781 ctgcgataac gcctccaatc gccgttacga tcaggaactt gcgtctgctc gggtggccgt
     6841 gcgatgccat atgaagagga tcgtgccagc agatcacgct cggaaagcgt gaactggcat
     6901 gcatttcgct ctttcgaatg ttgctgtgcg aacaaagtgg tagaccgggt cacgccgcgt
     6961 ggcgcagctc gtccagtgaa tcgagcatgt ccagcacctg acgcgcggtc ttgtcccaac
     7021 tgaagatctt cgcacgttcc agccccttgg cgctgagctc ggcccgcagg gtggcgtccg
     7081 ccagtacccg ctggatgccg cccgcgatct catcgggctg cagcgggtct accagacagg
     7141 cggcctgatc cacgacttca ggcaccgatc cggtgttggc tgcaacgatc gggcagccgc
     7201 acgccatggc ctccaccagc gggatgccga agccttcatg caaggagggg aagacgaaca
     7261 actcggccag actgtagagc gccgccaggt cctcgtgcga aatccatcca ggcatcagga
     7321 tacgatcttc caggcccagc tcacggatcg cgcgcagttc ctgtgtgtag ttggcgcgtt
     7381 gcgcgccggc cagcaccaga tgatgcggga tcacgagccg aagctttgca aacgcctgta
     7441 gcagccgcgg cagattcttg cgcgactcga tctgacccgc ccacagtatg aatcggtccg
     7501 gcagttggta gcgctgcgcc accttgcgca gatatgcggg gtcttctttg cgatggaaca
     7561 gggcagagtc gaaaccgtta tagatcacga atactttttt cggatcgacg tgcaaatgcc
     7621 tgacgacatc cgctttcacg cagttcgtga ccgcaacgac ggcgtcggcg cgctggccgt
     7681 acagcggcat catgcgctcg aaataccagc gatcgaaggt ctgaaaatgc tccggtatcg
     7741 cgaacttctc tgccccatgc accacgtagg ccttctttgc gccgctaagg aatgggatcg
     7801 tgatcttggg gttgaacacc atatccagat gccgggtgcg caccacccac ggcacggcca
     7861 cctgatccca aagcacggta cctgggatcc ggaccgcgat ctcttcgcaa ttgggatact
     7921 gggggtaggt gccgagccgc tttggatcct ggtagatgag aacgaagtgg tgcctgctag
     7981 gcagcgaaag cagccgcggc aggatgctat gtgtgtacac cttcacgccg ccatccttct
     8041 gatcgaagtg gcgcagcata ataccgatcc gcaaagtgcc tcccgacacg cgatgcgtga
     8101 cgcaaagccc ggccgaaccg acgctcgcga tgacgttagc acttcaacgt ctgtgtttaa
     8161 atcaggcctg gcagtttttg agcgcgtgca gcattgaaca ccgctcacga aaggttcatg
     8221 aaatctatcg aaagattgag aaagcttcat gaaggcgctg tcctacttca ctagcatgga
     8281 cgggggataa ctggcatggc gaacgactca aacagggact ggcaactgta cggtgaggtc
     8341 gatccgtatt tcggcgtcat cagcctggat cgattccaca agaacaatct gaccaacgct
     8401 gcgctgagcg aattcttcag ctccggagag cagcacattc aatacgtgct ggacatcgtg
     8461 cgcacgcacc tcgacccgac gttccgtccg atctgtgcac tggatttcgg ctgcggcgtc
     8521 ggccgatgca ctcttccgct agcacgatta tgcgagcagg tcacaggcgt ggacgtttcg
     8581 caggcgatgc tcgaagaggg ccaggccaac gcccgcaagc acatgttgtc caacatcaag
     8641 tggctcaatt cgagcgatct gtccaacctc aacggtagct tcgagttcgt gcattcgttc
     8701 atcgtgttcc agcacattcc gccggcccgg ggtatgccgc tgttggcgcg gctgatcgac
     8761 ctgctcgccc cagaaggcat cgcggcgatt caggtgctct accatcggga tgtatccagg
     8821 ctcaagcaat tggtcggtcg gctgcgtgtc agcgtgccgc tgatgcacaa tttagcgaac
     8881 ctgtactacg ggaaaccgtt caactatccg ctgatgcaga agaacgtcta cgacttgagc
     8941 gccgtgttcg atctgctgca aaagaagcag tgtggcagca gctgggtgac gttcgcccag
     9001 agcgatgaat tgcagtcggc aatgatgttt ttccgcaagc gcccggacgc gatgccctac
     9061 tatgagttct ataacgccgc taagaacgct cagtcggctt gagggcaagg tagacctttt
     9121 cgcgcggatc aatgccgtag cgcgcaaagt ccgcaggact gatcggaatg ctatccgcgc
     9181 gcgtctcgaa gcccaccgag cgtagccgct cgaggtagtc ggcctcggcg taaatcctga
     9241 catgcgtatc ctggccgaaa gcgcgcgagc gctcgtccgc ggtgcgcatg cggggatctt
     9301 cataggtcgt ctcgagcttc gcgccaatgg gcacctgcac gatggcgctg ccttggggct
     9361 tgagcacccg gaacagctcg ctcatcgcag tgcggtcatc cgcgatgtgc tccaggatgt
     9421 ggttgcagat cgcgaagtca aagcttccat ccgggaagtc aatgctgagc agatcgatgc
     9481 gatgtatcgc gcgcgaagtc gaatagtagg ccttgggatt gatgtcgccg cacacatagt
     9541 ccagacaccc ggatgccgcg atcaggtcat gcagtgcccg ctccggcgcg acatgcagaa
     9601 ttcgcttgcc gcgcagcgag agctgcttgc gaatgtagaa gtacagtccg cgctcccgat
     9661 cgcgtgattg gcaataagga cacttgaaga agctgatgga ctgtccggat atgatgtcgg
     9721 cggtcaccgc gatcggaaaa cggttgacgt aagggatgaa ttggccgaac tcgcgctgac
     9781 agaaagggca ggcgtgcgtg cgccccggat gggtcagccg ccaggtgaaa ccgcgggcaa
     9841 tctgtttggc gcgctgcatc gcgccgaact gagcaggcgg cagcaccaca tcggccagac
     9901 gcacaatgga cttaaggacg gtgatgcctg cctcctagcg cctaagcgcg tcccgggttg
     9961 atcgcacaat gatgggccgg ccggcgcaga gggtcaaaat cttttcgcgg cggccgtatt
    10021 caacggaccg cacgcgcggt cactcatctt ggggggcatt gtgtgtgaga tgtcccagat
    10081 gccgatcatc gcgatcgcaa acaccagcgc gcagcgcgcg aactgctcag taggcggtaa
    10141 acgggcgagg actagattcc cgcaacagaa tttgcgcgtg acttaactca cggcatgtga
    10201 cttatcacct gaatccgtcg gtttcgcgat gtgaatattg gatcccatca gttacactta
    10261 tttacaaaat cgcccgtgac atccgcaaga tactgagggt aatgaatttg aattggcgaa
    10321 aaccgctgat acgcgctgca ctcgcttttc agaaccccca tgtgttgcgc gaattgcggt
    10381 tgattcgctc aatcgagcga gccactcctg acacggttgc tgcggttcaa cgcgctcgac
    10441 tcacggcgtt gctgcatttt gcctggagcg agaccgacta ctaccgcgac ctattggacg
    10501 catgcggagc ggtccgagac ggccgcgtca atcttgatcg cttccaggat attccctttc
    10561 tgaccaagga cctcatccgc gcccaccagt cgcggatgag gcctcgatca cttccaaagg
    10621 gtcgatcggc tcacatcaat cgttccggtg gctcgacagg ggagccgctg cagttctggc
    10681 aggacaatgt ctactgggct gcgaccattg ccacgcgaac ctaccatttt tcgctgacgg
    10741 gtaaggatat cggtgagcgg gaaatgaaga tctggggtag tgagcaagac ctcttcaagg
    10801 gaaccatcgg agcgcgagcc aagttcgaga actggatcta caaccggcag ttcgagcaat
    10861 gctggcatct ccccgaggag aggatcctaa agatcattca cgacatcaac acctggcgtc
    10921 cgaagatgtt gtggtgctat cgggatggta tcgacgcggt ggcgcgatac gtcaatcaga
    10981 accgtatcag catgcactcc ccggcggcga ttgtgctcgg tggtgccacg gtctacccct
    11041 tcatggtgca agccatcgaa aaagccttcg aggcaccggc tatcagtgcc tatgggtcac
    11101 gtgagatcgg cgctgccgcc tgtcagtgtc tgaaggggca ggggcaccat atcgcgtcgc
    11161 atacccatgt catcgagagc attggcagcg acgatcagcc tcactacgag cgcgaagggg
    11221 acctggccat cacgccgttg ctcaactacg cgatgccgtt catccgctac cgcatcggtg
    11281 accgtggtgt gctcacgcgc gatctgtgct cgtgcggccg cagctttccg ttgctttcgg
    11341 cgttaaccgg tcggacggtc gaggtcttta ccaacagcaa gggtgaacag gtcgatccca
    11401 tctacttcat ccagctgctg tcggtcattt tcaatcaggg attcgtgcgc aagtttcagg
    11461 tggtgcagga acaggacggc ggcatcacga tcaattttgt actggcgagc ggtgtcgacc
    11521 tggatgccac cggatcgaac tttgacgata tcagaagtaa gatccttctc gtcatgggcc
    11581 gcaactgcct cgtgcgcttc aacgccgtgg atgacattcc gctcagtgcg tccggcaaat
    11641 atccctacat tgtgagccgg cgcagcctca agtctgccga gcgcacggag gaactgcagc
    11701 cgacctagct gtaggaacga gctgcgctcg cgccattgcc attaattgtc aagttcccgc
    11761 ccagcgggca tgtgaccgcg ttccgtgcgg tcacagggca agccgtggac aatgatcttt
    11821 ccaatctgga tggcagttcc agggccaaaa agcggatagg aaaaatgctc gactcgatgg
    11881 tcctgcggca acttggcgga cgaaccgcca ctcgcactct caactggcgc aagccgctca
    11941 tccgggccgc gtttgcactc aagaatccag gcacactggc cgaactggac ctgattcaat
    12001 ccatcgagag gagtgcgccg gagagtatcc aggctgtaca acaggcgcgg ctggagcagc
    12061 tgctgcatca tgcgtggagt cagaccgact actatcgcga ggttctggaa cagtgcggtg
    12121 tcgtgcagga cggtcgcatc aatctcgatc gctttgagaa cattccgttt ctcaccaagg
    12181 acatcatccg tacccagggc gatcgactgt gtgcccggaa attgccgcgc gggcgcaagg
    12241 cgtattccaa tctgtccggc ggctccaccg gcacaccgat ccgattcatg caggacagtg
    12301 gctattggga cgtcaccatt gcgacgcgaa gctaccactt ctcgtttgcc ggcaaggaac
    12361 tcggtgaacg agagatgaag atctggggca gcgagcgcga cattctgcag ggcacgctcg
    12421 gcttgcgcgc cagcctggag aactggctct ataatcggaa atttgaacag tgctttaaac
    12481 tgcccgagca gcagattcgc aagatcatcc gcgatatcaa cacctggcag cccaagctat
    12541 tgtggtgcta tcgcgatggc atcgacgcgg tcgcgcgcta cgccacggag cacgatatcg
    12601 aactgcattc cccggcggcc attctgttgg gtggagccac ggtttatcca ttcctcgcca
    12661 agcaaattga acgggctttt cactgtccgg tgctgagcgc ctatggctcg cgcgaactcg
    12721 gcgccgtggc ttgtcagtgt atacggggcc agggccatca tatcgcctcg caggcacatg
    12781 tactggagac catcaacgtc gccgaagagc cgatgatgga gcaggacggc gaactggtcg
    12841 tcaccccgct attgaactac gcgatgccca tgatccgcta tcgggttggc gaccggggcc
    12901 gcctgacgag gcgcctatgt tcctgcgggc ggaacttccc gctgctcgag tccctgacgg
    12961 gccgcatgat tgaagtgctg atcaacagta agggcgagca ggtcgatcca atttacttca
    13021 tgatgttgct cagcgaggtg ttcgccaatt cgccagtacg caagagccag gttattcagg
    13081 aaaaagaccg ctcaatcacc atgaatctgg cgctggacaa gggcgctacg ctcaatccga
    13141 gtaacccctt gctggtagct gcccggaaga aggtcgccgc ggtcatgggc accgactgcc
    13201 gcgtgcgttt cgaattcgtc gcagacattg cgttgaccgc gtcgggcaag tttccctacg
    13261 tactgcgccg ctagcggcag gttctccgcg accctcattg tccgctacgg ctcaatcgtt
    13321 gcgtcagggt gtctgccagt cgtagcgcca gcgccgcgat ggtgagcgtc ggctgcatgc
    13381 cgccgcctgt cacaaacact tccgatccgc acagatacag attcggcgcc tcatgcacct
    13441 tcagatcgcg atcgaccacg ctggagcgtg gattgtcgcc catgcggcaa gcaccctgca
    13501 gatgacgtga ccaggtgatt ccgatgtcct cgatgtcggt tgcccgcagt tgctgcagcc
    13561 agccgcgcag caacgcgcgc gcgcgctcga tcaatgcgcg gtcttcatcg gaatagtgga
    13621 agatcagctg cgccacaggc cggccaaacg cgtcagtgcg tgtcttggac aacgccacgc
    13681 gattggccgg tgagattttc atttcgatgc tggagccgaa atggattggc gcattcacca
    13741 cccgtttgaa cagcgacagc agcgtcgtcg ggatggccga cagctggaag cgcagcaggt
    13801 gattcggcat cagccaggac tgccggaaca ccgggacaat cgcacccagt ccctcgcttc
    13861 gaaaggtctg atagaactga tgcgttcggg cgatcttgtt cgtcggtgtc aatgtcaagc
    13921 gattatgagg tatgtgcgcc gagaggccga catttggatg ttcgttgaat ccgcgtccga
    13981 ccatgtcgtg ggcgttaccg atgccgttcg gaaacgcctc ggcggccgat agctgcaaca
    14041 gtcgtggtgt ctcgaagcct ccacagcaga caacaaagat tcgggcacga gcggtagcgc
    14101 cgtgaccgtc cagcgttttg acgtccgcgg ccacgatgcg ctgctgcgcg gcgacgaggt
    14161 tggtcacggt ggcgccggtg agcagggttc cgtgtccgct actcaggaag ttcggcagaa
    14221 tttccttctg tacgttgaag aaacgccagg tcttggttgg cgttgcggtt gcagagtctt
    14281 caacctcaac cccgaattgt gcacagagct gcgtgagcaa cgtgatatcc ggcgagcccg
    14341 gcaagggcag aggaccggtt cgcggcggcg cgaatcgggt gcgcgggccg ccgcgtacac
    14401 gcagcatctg ttctgcggcc tcgtagtagg gggcaagatc cgaatagcgg atgggccagg
    14461 gattatccgc gggagtgtag ggatgtggct cgaagtccga cggatgcagg cgctcacagc
    14521 ggccggtcca gaagttggaa ttgccgccca gcagtctgga ggccgtctga gtcagaggat
    14581 agtccgtgtc ccccgtgaac tcgtatctcg ccaggccctt cagctgtgca tcggtgagcc
    14641 agcttctcag tccccggccg gattcgagca atagcgtgcg aataccgcgc cgtgccagcg
    14701 tagtggcaag cgtagttccg gcaggaccag aaccgatgat gcagacatcg tactgctcag
    14761 atgcgttgag cgcatcgagc gatgcaagct gtgccatctt ttgccgtaac cctatcttgc
    14821 caattcactc tggcgcgtag accccatcgc aggggccgcg ctcttcgaaa agagcgggaa
    14881 cgccagccag ccgataacga gcgcgcgtac cagccagccg gcggccactc ccgacaccag
    14941 gccggcgagg ccccagcgcg atcctatcgc agcgccgact agcgataccg caattgtaag
    15001 ccaacccgcc atactgagtt tcaccaggtg cgatgcagag ccgagcgcat tgacggcgga
    15061 ggcgggcaag gtaccgacca ccttgaggat tccggagatg atggcaactg acagcaacga
    15121 gggcgcaatg tgatagcggc ctgcgagcac ataagtcagg aacaacggcg tgagccacca
    15181 tgctgcgaac gccgcgagta cgcagctggc agcgatgatc gccgtttctc gtccgaatac
    15241 tcgacgccgg gcgcgcgcgt caggcgcatt gcgcaacgca ggtaccagag taaagctgac
    15301 accctggtaa aggatcgtga agggcgagcc cgccagagtg gccagtactg cgaaggtcgc
    15361 gagagtcggc aggtcgagca attttgggac ggtgatacgt tccagcgatg tcatgatcat
    15421 gctggcggcg atgaagtttg ccgcgtgcca gctctcgttc cagggatagg actgagccgt
    15481 attttgctgg actcgctcgc gatcagatac aacggaatac cacccgatca gtgccgcgct
    15541 acacatcgtg accgtcatga cggtcgtcgg tagcagtgca ctcgaccggg ctacgaacat
    15601 cgaggcgaca ccggcaacta gaattgatgc gttggtactt gcggtcagcg cgagcgcggc
    15661 caggaatctc tcctgacttc tgtaatacga ggtggcgacg accttgacgc tgcctgcgac
    15721 ggtcgtgatg aacacggccg ccagcaacgc aaagctgagc ggatagagcc atatcgcggt
    15781 cagaatcagc agcaggccaa cagcgcagga ggtcaggaac acttgcaggt ggagctttga
    15841 gctcgtgtca tagtgattgc gagtcagtat gacgtcggca cccatcggcc cgacgttgac
    15901 gccgatcatc accagtgcaa acaccagtga aaagctcgcc agatgttcag gcgataggac
    15961 gcgcgcaagg agcagatttc caatcgccag cgccgcgccg ctggccccaa acgcagacgc
    16021 tgcccgtagc gatttggatt taaacagcga tcgcagtacg cccgtcggca tcagttcgag
    16081 ccggaagacg aagtccgtgg ctgcatgtcg cgcacgcggg gaaacatgat gaatgccagc
    16141 aggtagagcc cgagagcgag caggatggca aagttaaaac catacagcag gcttaacacg
    16201 atgctcatga acccaccgac gacggtaaac agcccgttca tgccccaggc ccaggcaatc
    16261 gcgccgcgcg gctgattgct gatggcaagg atgcccagcg ggaacggcat acccaggaag
    16321 aaacccagcg ggaaaatcat ggctgccgac gccaggactc gcccggtgac aggcaacgcc
    16381 aaggcgatgc gtgccagcga cgggtagaac gcagtaaacg cgaggccaat caccagaatc
    16441 gccgtgaatg gcagtgccca gcgctggcgc gtcgtaaggc ctaaacgctc ggacgcgacg
    16501 ctaccaacgc ccgccgagaa cagcatggta aatatgacgg tggagtaggt gtagagcggg
    16561 ctgccgatca ggtgtatgaa tttctgaata gataccagct cgatgatgat gaagccggcg
    16621 cccaagcacg agaaatatac cagcaggggc actgccgccg cgcgctcttc tcgaccgacc
    16681 ttcgagaagc gcaaaggtac gaaaacgaac acgatcacga atatgatcga agccacgccg
    16741 gtcacgatca gatggatcag atccatcgga atgatcccct tgatcaggga gctgtttaga
    16801 gcatacgccg tgccttcatc cagatagttc gacggatcgg gcttgagttc gcggatcgac
    16861 ttgcgtgtca gaccgaaata cggccgattg tcggtgcgtg gcgtgacgtt cgccgccatt
    16921 cgctccgcga ggctggccgg aaaatcgccc gagtagaaat cagctgacag aaagctcttg
    16981 gtgcggtcca gcgggttctc cacaatgtgc aaccggtagt agtcgctgag ctctgatggc
    17041 gcgagaaatg cgctcaggtc ggcaatctcc gcagcagtcc agggctgcat cttgatgagc
    17101 agcgtgggca acataggttc cgactcggag aagaagaccg tcacgtgtga ggcaagatcg
    17161 gttctgccca tccgtttcca tgcgagcgct gcggtcgaga tcagtctcgg gaaggtattg
    17221 tggttgacct gcagtacgcc gttggcggac aggtgagaga agtattcttg gtacgcttcc
    17281 gccgtgtcca gatagtccgg cgcgagcgct ccggtaccct gcgccacgct cgagctggtg
    17341 aagttgctgt aaatctgaat gatgtcgtag ctgcggttgc tgtgtcgaag gaagctgcgg
    17401 ccttcgcccg cctggacgtt caccgccgaa ttgtgaaaga tgttgccgat gtagtcggag
    17461 taggatccgg tcgcgagttc gaccacggtc ggcaccagtt ctaccgcatc cacgtgtgct
    17521 gcgccgtaga tcagtgcggc cttggtttcc tgtccaccgg cgctgccgat gatcagtacg
    17581 ctctgattcg agtcgcgctt caggtagtgc gcggccaaca ctccgagctg ccagaaatgc
    17641 tccttaaccc gtgatttatc aatatcgagc cgggctcgca gcgccttgag atcgccatcg
    17701 aacggaaaga agtaactggt ttggttgcca ccgtcatatt gaatggcttt gcggtttccc
    17761 atcggccacc agggttgcgc tttctcaggt gtccaatcct cgcgtaccac gttgatcttg
    17821 gagatcgggt cccagcgcac gaactcgccc attcccgact gcagggcgcc aagcagtccg
    17881 cgcttgtcca tgtgctgctt gaaatcgatg tactgcggcg cgcgcaggat aggcgcggcc
    17941 aggatcactg ccgccagcac gcatgcgctg atcgacgccg cgcgcgaggt ggcaaagagc
    18001 gccgcggcaa tcagcgccag gcctgcagcg cagatgatca gcccgccggg gccgagtttt
    18061 gaaatgagcg gtaccaccag gatcgagcca acaccggcgc cgatcaggtc ccagaagtac
    18121 agacgctgaa tgctttgcgc gtacttggag aacactgcga tcaggatgta gccggccaga
    18181 gcaaacggga tcaacaggac gaagtacagg acaatgaaag cactgagagt ggagaacggc
    18241 gctcggccca atcgcgtgta gtccaatggc agggcgttga tgatcggtat cagcaccaag
    18301 atcgcagcgg cgtaccagaa actgcgattg ctgagcatgg ggcggatttc actatcgacc
    18361 gctatcggtc gcagggtggc atagacgcca gccagtccaa acgagaatac cgccagggtt
    18421 accacgaaat aagagatgtt cggtgccagt gtcacgtcaa atgcgcgggt cagcatcagt
    18481 tccagaatca gggtcgaaag agccgtcaaa aagacgccga acgacagtct tcgcatctca
    18541 acactcccag cgctcaatag gcggaacagg taacgcgggc gcagcataca ggagcgcgct
    18601 tcgacagcgc ccgcatgtcg ccggtgaatc gagactcgcc gcacacaggc acggcgcacc
    18661 gcgcgagccg gtcgtgggga tgatcagtgg catatgatgc gatgcaccat cactttcgag
    18721 tcagccttat gcagcgtcgc aattgtccgc aatcataaaa ccgcgataat cgctgtcccg
    18781 actttgacca cccacaagac cctcggagga attaagtgcc gcgacgcaag gaattgaccg
    18841 cgacgataga accgttcgac tcattctggg aagcgccgga tgatatcgag aaggggtatc
    18901 acagcttctg gctcttctac aaggataact accagaagta ttttccgagc gataagcagg
    18961 ccaagattct atgcgtgtcg tgcgggccgg gctacggcgt caaactgctc gcggaccttg
    19021 gctatcaaga cgtcctgggc atcgattcct ttgaagacaa gattgccttt gccacagcca
    19081 agaagctcaa ttgccggatg ggctacgcgt ttgagttcct gcaagacgcg cccgatgagt
    19141 cctacgatct gatttggctc gagcaggagg tcaatcacct cacgcggtcg gagatcctcg
    19201 agtttctggc tctgtgtcta cgcaagctgc ggcggggcgg gcgtctgatc gcgttcgcgc
    19261 tcaacggtgc gaatccaatc accggagctg aggcgctggc gcagaatttc gatcacttca
    19321 acacatttac cgagtacacg ctgcgacagg tactcaatca cacaggattc gagaagattg
    19381 aggtgttcga tctcaatctc tacgtgttct acaagaatcc attcaactac gtcggcatgg
    19441 cggcatcggc ttttctgtca atgctgtttc gcgcttgctt tctgctctat ggcaagaaga
    19501 accgtctgtt tggaaagaag atcgctgcga gctgcctgcg ccctgcttga atgaatcctg
    19561 caccggggtc cctggtattc gatcgatggt gcttttttgt acataaccga ttgaatcgat
    19621 tatgtttgta ggatggcgcg gcaagctagg tttagggtat cgtccgaact gtcgccgttg
    19681 tgcgtcaaaa gttggcacgg atgtcgcagt tctcacgcgg gaatcatgtg agttcaacgc
    19741 ttagtcgata cactttccag ctccggatta ggggatggtt tccttcgata acggtgcctg
    19801 aataatgccg ctagagccct cgctcggcga tacgccgcgt acatcgtccc tgaaccgact
    19861 tgccaatgag acctttgatg tgctcgtcat tggtggcggg attggtgccg cgtgcacagc
    19921 gtgggacgcc gcgttacgtg gactacgcgt ggcgctggtc gagcgcctgg actttggctc
    19981 gggcgcgtcc gcgcattcat tcaaggttct gcacggtggc atccgctatc tgcagcatct
    20041 ggatatcgtg cgtttgcgcg agtcatgtcg cgagcgcggc gcgtttctgc ggatcgcacc
    20101 gcatctgact cggccgttgc cgtttgcagt gccgacattt ggctacgcaa tgcaaagccg
    20161 ttgggtgttc ggggccgcgc tggccaccct cgagacgctg acctgtgatc gcaaccgggc
    20221 gacccgcgac ccgttgcggc gcgtcccaaa gtcgtatttg ctgacgcggg acgagttgct
    20281 gcgccgtttc gaaaacgtcg acggcgatgg gctgaccggg gccggagtgt tctttgacgg
    20341 acagatcctc aatccgccgc gtgctgtact cgccgtgatt cgatccgcag cgagtcacgg
    20401 agcggcagtg gccaactatt gcgaagccca ggagatggtg gtccgggatg gccgcgtcgt
    20461 tgccatgcgt gcacgcgacc tgatccgggg cgaggtgttc gacatccgcg cacgcgtgat
    20521 catcaatgcg accggtccgt tcgcaccctc cttcgtcaga cggttggggg caggcgtcca
    20581 actcaacgtg ccgttgtcgc gcgacatggc attcgtcgtg cgtcgtgtgc tcgatccgga
    20641 gatggcggtc ggggtgcaga cgcggtatcg cgatccggac gccatgctca gccgcggcaa
    20701 ccgacacctg tttatggcgc catggcgcgg tcgctatacg ctgatcggag tcaacagccg
    20761 tgtgtacacg gaggatgcct acgacttgca tgtgaccgag ggcgagatcg aaggattcct
    20821 acaggagatc aacgaggcct ctccaccgct tgccgtgcgg cgcgacgagg taacggtggt
    20881 caacgccgga ttgctgccgt ttggcgagaa cagcccggac cagaaggatt tgagctttgg
    20941 caagcgctca cagatcatcg atcatgcttc gaccggtggc ccggaaggat tggtgagtgc
    21001 catgagcatt cgctggacca tgggacgtct gctgggagag cgggtagtcg atctggcgca
    21061 gcagaaactt ggcatcaagg ccgtgccctg cagtaccacc acgacgcgcg tctggggcgg
    21121 tgacatcgag tcggtcgaag gctatgcgcg cgaaatgcgc cgcttgctgc ccgcagcggt
    21181 gagcgatgct caggcgacgc gcatgtttca gcactatggc accaagtggt cggaagtact
    21241 ggcaggcgac gaccgcgaag tgatccaggg cgatatgatc ccggcaactg actatttcgg
    21301 cgccgaggtg cgttacgctg tgcgcaatga aatggcctcg acgctctcgg acgtcgtgat
    21361 gcgtcgactg ggcctgggtt cggcggaacg cccggcggat gagaccctgg ctgcctgcgc
    21421 atcgattgcc ggcgatgagt taggttggag cctggcacga cgtgagcgag aaatacagag
    21481 cgtgcgcgac agctttccgt tcgccagccc gcgttcgcag tatccgagat tcgattcatg
    21541 agcgcgcagt tccgcatcat ctgtccgacc tactggtatc cgggcagcgc gaccgatatg
    21601 cgcgcgatct acgtgcacga catcacgcgt catctggtgc agcggggcca tcgtgtctgc
    21661 gtggtcactc cggccggcac cggcgcgccg ctcagagata cgctcgacgg cgtcgagatt
    21721 catcgctttc cgctggcggt acctgaagat ctcgcctacg gcaaagtcgc acagagcaaa
    21781 gttggactgg tcagccgcat ggtgcgcatg caggtcatgg tccaatacct ctggcgtcag
    21841 tatcgtgcga tgctcgattg tgcgcgggat ttcaagcccg acgtggttca cgggcactgg
    21901 gcaattccga ccggtccggc attggtgtat ggcagtcaga agttgcgaat cccgtcggtg
    21961 atcacgatgc acggcggtga tgtgtacgtg aacaaggcgg agggctacga ctttccgact
    22021 cggccctatg tgaagccggt tttgcactgg acgctgacgc gcgcgtcagc gctgaccgcc
    22081 atcagcgatg actgcgtgca ccacgctctg cgcgcgggcg cctcgatgga aaagatccac
    22141 ctcatcatga acggcgccga tctgcgacgc ttcagcccca gtggcgaaac gccggatcgc
    22201 ccctatggca acaagatggt ctttgcctgc aggcaattgt ttccgcgcaa ggggatccgt
    22261 tatctgatac aggctgtggc gcggctcagg ccgcagcatc ccgacattcg gctggtgctc
    22321 gcgggcgatg gctttgagcg cgccgaactg gaggccctgg ccgacaaact gggggtcggc
    22381 gacatcacga cgttcctcgg ctgggtagtc aatagcgatc tgcccaagta ctttcgcgca
    22441 tgcgcggtct cggtcatccc gtcgctggaa gagggatttg gcatcccggc agctgaagcg
    22501 atgggatgcg aagtaccggt cgtggcgacc gatgccggtg gcttgcctga ggtcgtggcg
    22561 aacggcgaga caggcttcgt tgtccccaag gccgacgcga acgcactggc agaggcgatt
    22621 ggcaagctgt tgaccgattc ggccctggca catcgcatgg gcaaggcagg cagacagcgt
    22681 gcgctggagc ggtttgactg gatgcgttct gctgaacgct tcgaggagct ctacacgagt
    22741 ctgttgtcgc ctgccggggt cgcctcatga atgagcgcgt attttccttc cgctgggaca
    22801 tcgaccatcg cgcctgcatc accgatggcc tgccgaagat tctggatgtc tgcgatgagc
    22861 tgggcgtcaa gaacaccttc ttcgtgaata tgggccgttc caccaatctg cgcgagtggc
    22921 tcggcaaggg tttgcgcggc agccgcgcga aactcaacga tatggaggcc gtccacctca
    22981 tcaagaagat cgggtgggcg cgctttggcc ttgaaacgct gctgtcgcga cccgttggcc
    23041 gctctctcat caagcccctg gaagaattac aaaatcgtgg ccatgaactc ggtctacatg
    23101 gcggcagcga tcatgtcatt tggagtcgtc gtttcgcgca actctccgcc gaggagatcg
    23161 aagcagacgt cgcgcgcaca ctcgaaacct atacgcgcta tttcggcaaa cccgcaggct
    23221 tcacatctcc cgggttcaag catgacgagc gaaccagccg gctggtagag aagcttggct
    23281 tcaaatacga cggcgatgcg ctcggcggtg agccgcacca cccaaccttt gacggaaaac
    23341 ctggaaagca ttggacgatt ccagtgacgt tgtgcggacc gcggaccatt ccactcatcg
    23401 aatggcacgg cgcacgcggc gtctgcgagg atgagatcgt cgccgatgtg atgcgtcgca
    23461 tggccgacga acgctgggtc gtgctgtacg gacacccttg ttacgaagga gtgcgcgcgg
    23521 gattgctgcg acggatcttg cgcgcagtgc aggaagccgg tttcaagttt ctgactcatc
    23581 tgcagatggc ggagcgcctg tatgccggta cctaatttgc cattgagggt tctgtcggtc
    23641 ttcggcactc gccccgaggc gatcaaaatg gcgccggtcg tgcgcgccct cgagtcgcag
    23701 tcgcagcact tcaagagtct gatctgcgtg accggtcagc atcgcgagat gcttgatcag
    23761 atcatgagcg ttttcaaatt gtcggccgat ttcgatctgg acgtgatgcg cgcgggtcag
    23821 acgcccgccg aagtcatgag ccgcatcctg gcggcgatgc cgggcatcct gaatcaggca
    23881 aaaccggatc tgatactggt ccagggcgac acgacgacga ctgcggcggt ggcgcttgcg
    23941 gccttcttgc aacgcattcc ggtcggccat gtcgaggccg ggctgcgcac cgacgacatc
    24001 agccagccat tcccggaaga gatgaatcgc cgtgtcgcat cgctggtgac cacactgcat
    24061 ttcgcgccga cgatgcgtgc ccagaacgca ctgctgcagg agcgcgtacc cagcgagcgg
    24121 gtgatcgtca ccggcaacac cgtgatcgat gcgctgctgc aggcgcgccg gcccgactac
    24181 cgcttcgtca attcctcgct ggcgacgctc gatgtcacgc gccgggtcgt gttggtcacg
    24241 ctgcaccggc gtgaaagctt tggtgcgccg atgctgtcag tgtgccgcgc actcaatcgg
    24301 gtcgctcagg gacatccgga tctcattttc gtgctgccgg tccatcgcaa tccgagcgtg
    24361 cgggaagtgg tcgttccggc gctacagggg tgtccgaatt tcatgctgat cgatccgctc
    24421 gactatctgg attttattca cgtcatggcg cgctcgcgtg tcatcgtgac cgacagcggc
    24481 ggcgttcagg aagaggcgcc gactctgaat gtgccggtgc tggtggcgcg cgaagtcact
    24541 gagcgccccg agggcgtgga ggttggcgcc gcgcgcctgg tcggcaccga cgagaaccgg
    24601 atcgtgcaga tgctggagga act
//
LOCUS       DATMRM010000050         7872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4365686, whole genome shotgun sequence.
ACCESSION   DATMRM010000050 DATMRM010000000
VERSION     DATMRM010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7872
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4365686"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VNZ06_01415"
     CDS             complement(<1..965)
                     /locus_tag="VNZ06_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19437.1"
                     /translation="MGVEVKLTDRELPASPAFIKFLAQTLRDRSWHSEVWADQRSEAL
                     SYSNDDLERQQCFEAAELVMQAASGRGWVARAYELLYCLMVGNLEPLWELQRRFRFVA
                     IIGIARSGGSYLTAELYRAIGIAPDRVPGALAHDGFPDIGPFVLAPGGNSWTTSLKTM
                     AEYLVMVEIFFDSRAVHEGKIVVPKKLTKGTYAGDFIQHSLGEDAEYLLTVRHPVAAC
                     VSTYEKSSGLPANGRFAVRSNIEGWCQRDLLHTGCGAQELVRMDYFDAYLRYWEHYHL
                     CLVTSGFASAAKLRVVAFGDAALRSVAQSYHDRYDSARRAGEFHTS"
     gene            1131..1910
                     /locus_tag="VNZ06_01420"
     CDS             1131..1910
                     /locus_tag="VNZ06_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19438.1"
                     /translation="MITARRLLPAVAVSGSALVVGCGGYSGSCGGSYGSSYGSCVAPS
                     VDATGIYQGTLLDNQTQQQTSAVALIAENGEALVSTQAGAYYHLNVGSVGSELSGSYQ
                     AYSSSANFPNGSHADAGAIDATVSSAGLTGTLKDNSGDVESLNLSFDNIYSQPSSLPT
                     LAGTWTYASGGFTLTVTIQANGVLSASDSNGCTYAGGFGIIDSRTDVYSVSYTRLCSG
                     IGVTLNGVAAYFPAGGAAAPADIQLLADDGAGDYLSADLQQ"
     gene            1867..2826
                     /locus_tag="VNZ06_01425"
     CDS             1867..2826
                     /locus_tag="VNZ06_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWW19439.1"
                     /translation="MTVPATIYRPIFSSNSDTHRGPGGGSSEQGVSVALRDILVHLDQ
                     SERGSVRLRLAVDLAARHGSRLTALYTPEMTPGQMASLRAAELGLASAEAMAKFNQTV
                     HTDTSERRAQLQAALTKLCGGLHLDFDWQEIDGLASIVLPQYARLADLCVVGQDGRVS
                     SESIGYEFSEQLLFVTGRPVLLVPAAGMFSTLGRHIAVAWNASRAASRALHDALPLIE
                     RAEKVTVLAADGAHLPRRPGALPVRAIIEHLHRHGVAAELVDINVPPDHSIAAALLAE
                     AQRLSADLLVSGAFGHPRLWEKLLGGVTQDLLRHMNLPVMMSH"
     gene            complement(2990..4516)
                     /locus_tag="VNZ06_01430"
     CDS             complement(2990..4516)
                     /locus_tag="VNZ06_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWW19440.1"
                     /translation="MQYRNILGLGLLAAGLTACVGPNYHTPKSELPKQYIAATDLPAS
                     TGTTAPGVDLAQWWHALNDPELDSLIERAVRANPDIEIALTRLQQARTRQVIYAGDGL
                     PLVAADTAAGHGTGSDFARAGALPPLRAGDNKGNLPQIAQVAGFAATWEIDLFGGVRR
                     EIEAGRYDVQAAAAARNAVLISVVADVARNYVELRGLQMRLAILRDNIATAGQSRDLQ
                     QARFSRGLTNELDLQLAIREYSTLESELPMMQAAIDTSEANIAVLLGLYPEDLSGELS
                     KPGVVPDVPAGLKAGMPVDLLQRRPDIGEAERELASATALIGVATSNLFPHVALNAGL
                     GTQSDTITTRHGSHIWDFGPSVYWPLLDFGTLDAQVSVAKLLAHQRLVAYRKTVIDAV
                     QDADNAISNYSAQQQRLTDLTDALTASQRAVNLAQQRYDRGLTDFLNVVDAERQAYAL
                     ADQYTASQESAAEAFIYLYRALGGGWEHYQDLPPIHHPLPAVLAGLRSLVTGYNPEKQ
                     "
     gene            complement(4520..5758)
                     /locus_tag="VNZ06_01435"
     CDS             complement(4520..5758)
                     /locus_tag="VNZ06_01435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWW19441.1"
                     /translation="MPSNDTGRRLQRAAIAVAVILAIAFAAEFLIRLHARHLLAKESQ
                     ASARAVPAVDVVVVRDNTGTGTLTLPGETAAWYESTIYARVDGYVAKWYVDIGDHVKA
                     GQLMATIDTPELDAELMAAKAKLQAAQAQVKVSEATAEFASTTYARWRDSPKGVVSEQ
                     EREDKKAGNDAAAAQLQAARAQVSLDEADVNRLSAFEQFKRVTAPFDGTVIERRIDIG
                     NLVTAGSSGNTTSLYRVSQDDPIRVFVDVPQSAAPNLMKVGVAAQISISDSGAPPIAG
                     KITRTSEAVNPQARTFRAELDIPNPKRALVPGLYVQVAFQLNNSGMYQVPAASLTFTP
                     SGPKVAVVNPDNQVQFHPVTIGRDDGDHVELSSGVASGDRLVLNISNQIADGQRVRIT
                     DTPGSPTDTEEGSSVAMAAH"
     gene            complement(5748..>7872)
                     /locus_tag="VNZ06_01440"
     CDS             complement(5748..>7872)
                     /locus_tag="VNZ06_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW19442.1"
                     /translation="LAAIPLSIVTAILVMYVGGQTLNTMTLGGFALAVGILVDNGTVV
                     IENIERHLSLREPLVEAIVNGASEVGIPTFLATLCICIVFVPVFLLQGTAKYLFSPLS
                     LSVCISLLASLVLSFTMVPVLFFHLMRGHTATGPLPDPEPVHGATGARRNPFMAIHTG
                     FIDRFERFRTVYRDALNWALKEPLRTALFFGVLIVCSLMLFPALGRDFFPQVDAGQFR
                     LHVRAPPGTRLEETQRDFAEVEAAIRNIAGNAQIDTILDNIGLPYSGMNIALSDSATV
                     GPMDGEILVSLKEKHTPTAQLIAKLRRELPQRFPDMSFFFQPADIVDQVLNFGQPAPI
                     DIRISGPDSNAAYAMANKMVRDLHHVPGVVDSHVFQVPDAPTLDVNVDRSMASQLGMA
                     QRSTANDLLITTNSSAQSAPNFWVDPKNQVSYPLVVQLPTYHIDSSHDLWTMPVSGGD
                     GRNSRELLMNVASFNRGVAPLVLSQLNIRPVFDVDADIQGTDLYSAAAAIQQVIAADR
                     PPASSAMTVTLSGQIETMLDSYTGLFSGMALAVMLVYLFLVINFQSWLDPLIVLLAVP
                     FALGGVMWMLYLTGTHLSVPALMGTLMCIGLTTANSILVVTFANQRMDAGDDSDVAAA
                     AAGYTRLRPVLMTAGAMMLGMIPMALGVGEGGEQNAPLARAVIGGLLFATFATLVFVP
                     TMYRVLRRRPVAHSGLSTTGDPLAI"
BASE COUNT         1544 a         2411 c         2425 g         1492 t
ORIGIN      
        1 gaggtatgaa actcgccggc gcgtcgtgcg ctgtcatagc gatcgtgata actctgcgcg
       61 acgctgcgca gagcggcatc gccgaatgca accaccctca gctttgccgc cgaggcgaag
      121 cccgaggtaa cgaggcacaa atgatagtgc tcccaatagc gcaggtaagc atcgaaataa
      181 tccatacgca caagctcctg cgcgccacag ccggtgtgca gcagatcacg ctggcaccac
      241 ccttcgatgt tggagcgcac ggcgaaccgt ccattggctg gtaatccgct cgacttttcg
      301 tatgtggaga cgcaagcagc cactgggtgc cggaccgtca gcaggtactc ggcatcctct
      361 ccgagtgagt gctgaatgaa gtctcccgcg taggtgccct tggttagctt cttcggcacc
      421 acgatcttgc cttcgtggac cgctcgactg tcaaagaaaa tctccaccat caccagatat
      481 tcggccatcg ttttgagact cgtagtccag ctattgcctc ccggtgcgag cacgaacggt
      541 cctatatcag gaaagccgtc atgcgccaac gcgccgggta cccggtccgg tgctattcct
      601 atggcgcggt agagttcggc ggtcagatac gagccgccac tacgggcgat gccaatgatc
      661 gctacgaagc ggaagcgtcg ctgcagttcc cagagcggtt caagattgcc gaccatcagg
      721 caatacagca attcgtaagc gcgggccacc caccctcgac ctgacgccgc ctgcatgacc
      781 agctccgcag cttcgaagca ctgttgccgc tccaagtcat cgttggagta cgaaagcgct
      841 tcggagcgct gatccgccca cacttcgctg tgccagcttc tgtcgcgcaa ggtctgtgcc
      901 aggaacttga tgaacgccgg ggaggcaggc agctcgcggt cggtcagctt gacttcaaca
      961 cccatgtttg aaaggtgaga ccaagataaa gaaacattaa aaggttaatt tatattaaaa
     1021 aggtgacacg tatcggaaat attcatcgac gatgtcaaag actactacaa tccactcagg
     1081 ctttccggcc aaggaaccga tggagtgagg cagttgcatt ggtgggtccc gtgatcaccg
     1141 cacgtcgact gttacccgcc gtggccgtct ccggcagcgc attggtggtg ggctgcggcg
     1201 gctatagtgg cagctgcggc ggctcctacg gatcatcgta tggaagctgc gtcgcgccca
     1261 gtgtcgatgc cacaggaatc tatcaaggga cgctgctcga caaccagacg cagcaacaaa
     1321 cctccgcggt agcgctcatc gccgaaaacg gcgaagctct ggtgagcaca caggccggcg
     1381 cctactatca tctgaatgtc ggctcggtgg gcagcgagtt gtcgggcagc tatcaggcct
     1441 actcgagttc ggcgaacttt ccgaacggaa gccacgcgga tgcgggtgcc atcgatgcaa
     1501 ccgtcagctc cgctggtttg actggcacac tcaaggacaa ctccggcgac gttgagtctt
     1561 tgaatttgag cttcgataat atctatagtc agccgtcttc gctcccgacg ctcgcgggca
     1621 cctggactta cgcgtcgggg ggattcacgc tcacggtcac tatccaagcg aacggcgtgc
     1681 tgtccgccag cgactcgaac ggctgtacgt acgccggagg cttcggcatc atcgattcgc
     1741 gtaccgacgt ctacagcgtc agctataccc gcctctgcag cggaatcggc gtaacgctca
     1801 acggtgtcgc cgcttacttc cctgcgggcg gcgccgcggc accggccgac attcaacttc
     1861 tggctgatga cggtgccggc gactatctat cggccgatct tcagcagtaa tagcgatacc
     1921 catcgcggac ccggcggtgg ttcgagcgaa cagggagtat ccgtggcact cagagatata
     1981 cttgtccatc tggaccaatc agagcgtggc tctgtgcggc ttcgtctggc ggtcgatctt
     2041 gcagcccgac acggaagccg gctgacggct ctctacacgc ccgaaatgac ccccgggcag
     2101 atggcatcgc ttcgagctgc tgaactcgga ctggcctccg ccgaggccat ggccaagttc
     2161 aaccagacgg ttcacacaga taccagcgaa cgccgggctc aactgcaagc cgcgctcacc
     2221 aaactgtgtg gtggccttca tctcgacttc gattggcagg agatcgacgg actggccagt
     2281 atcgtcctac cccagtacgc gagactcgct gatttgtgcg ttgtcggcca ggacgggcgc
     2341 gtcagttccg agtcgatcgg ctacgaattc tccgagcagt tactctttgt gaccggccga
     2401 ccagtattgc ttgttccagc cgcaggaatg ttcagcacac tcggccgaca catagccgtt
     2461 gcctggaatg ccagtcgtgc cgcatcgcgc gcgctccatg acgcattgcc actgatcgag
     2521 cgtgccgaga aagtgaccgt acttgcagcg gacggcgctc acttaccgcg ccgccctggt
     2581 gcgctgcctg taagagcgat catcgagcac ctgcatcggc acggtgtcgc cgcggaactg
     2641 gtggatatca atgttccgcc ggatcactca attgccgccg cactgttggc cgaggcacag
     2701 cgactcagtg cagacttgct ggtctcaggc gcgttcggcc acccccggct gtgggaaaag
     2761 ctgctgggag gcgtgactca ggatctgctc aggcatatga acctgccagt gatgatgtcg
     2821 cattgacgat cggtataccg atagagggcg tcaggcgctg ggccggccgc ccgcggtcgg
     2881 ttgcgagggc aggacgcgaa catccagcag tgcgaacgta ctgagaccac cccaggatca
     2941 gcgcgccgag aatgctgggc aacgagacga ccggatccat gctcaatcct tattgctttt
     3001 caggattgta tcccgtcacg aggctccgta accccgccag caccgccggc agcggatgat
     3061 ggatcggcgg cagatcctga tagtgttccc agccacctcc cagcgcgcga tagagataga
     3121 tgaaagcttc ggccgcgctt tcctgtgacg cggtgtactg atcagcgagg gcgtaggcct
     3181 gccgttcggc gtcaacgaca ttgaggaagt cggtgaggcc acggtcgtag cgttgctgcg
     3241 cgagattcac cgcgcgctgg ctcgcagtca gtgcatcggt cagatctgtg aggcgctgtt
     3301 gctgagccga gtagttggaa atcgcgttgt cggcatcctg gacggcgtcg atcaccgttt
     3361 tccggtaggc gaccagacgc tgatgtgcta gcagcttggc gacgctgacc tgtgcatcca
     3421 gcgtgccgaa atcgagcagc ggccagtaga cgctcgggcc aaagtcccaa atgtgactgc
     3481 catggcgggt cgtgatcgtg tccgactgag tcccgaggcc tgcattcaat gccacgtgcg
     3541 gaaacagatt cgaggtcgca acaccgatca gtgcggtcgc gctcgccagc tcgcgctcgg
     3601 cctcaccaat atccggtcgg cgctgcagca ggtccaccgg catgcccgcc ttgagacccg
     3661 ccggtacgtc ggggaccacg cccggttttg acagctcgcc gctcaagtcc tccggataaa
     3721 gacccagcaa caccgcgata ttggcctcac tggtatcgat tgccgcctgc atcatcggca
     3781 attcagactc aagcgtggag tactcacgaa tggcgagctg caggtcgagt tcgttggtca
     3841 ggccgcggct aaagcgcgcc tgctgcagat cgcgcgattg tccggcggtc gcgatgttat
     3901 cgcgcaggat ggcgaggcgc atctgcagtc ctcgcagttc aacgtagttg cgtgcgacgt
     3961 cagccaccac gctgataagc acggcattgc gtgccgctgc agcagcctgc acatcgtatc
     4021 gtccggcctc gatttcacgg cgcactccgc cgaacagatc gatttcccag gtggcggcga
     4081 aacccgcgac ctgggcgatc tggggcaggt tgcccttgtt gtcgcccgcg cgcagtggcg
     4141 gcagcgcgcc cgcgcgggcg aaatcgctgc cggttccatg tccggcagcg gtatcggctg
     4201 ccaccagcgg cagaccgtca ccagcgtaga taacctggcg cgttcgtgcc tgctgcaacc
     4261 gcgtcaacgc gatttcgatg tcggggttgg cgcgcacggc gcgctcaatc aatgaatcaa
     4321 gttccggatc gttcagtgca tgccaccact gcgcgaggtc gacgccaggg gcagtcgtac
     4381 cggtggatgc cggaagatcg gtcgcggcaa tgtattgctt cggcagctcg ctcttcggag
     4441 tgtgatagtt gggacctacg caggcagtga gccctgccgc tagcagtccg aggccgagaa
     4501 tattccggta ctgcatgcgt cagtgcgccg ccatcgcgac actcgagcct tcctcggtat
     4561 cggtcggtga cccgggggtg tcggtgatgc ggacgcgctg tccgtccgca atctgattgc
     4621 tgatattcag caccaggcgg tcgccggacg caaccccgga gctcagctcg acgtgatcgc
     4681 cgtcgtcgcg gccgatcgtg actggatgaa attgcacctg attgtccgga ttcaccaccg
     4741 ccactttcgg tccgctcggc gtgaaggtga ggctggcagc cggcacctga tacatacccg
     4801 aattgttgag ctgaaaggcg acctgcacgt acaagcccgg caccagcgcc cgcttggggt
     4861 tggggatatc gagttccgcg cggaacgtgc gcgcctgcgg attgaccgcc tccgatgtgc
     4921 gcgtgatctt gcccgcgatc ggcggcgccc ccgaatcact aatcgaaatc tgcgccgcga
     4981 cccctacttt catcagattg ggggccgcac tctgcggtac atcgacaaac acgcggatcg
     5041 gatcatcctg cgagacgcgg tagagtgagg tggtgttgcc ggagctcccg gccgtcacga
     5101 gattgccgat gtcgatgcgg cgctcgataa ccgtgccgtc gaacggggcg gtcacgcgct
     5161 tgaactgctc aaaggccgac aggcggttga cgtccgcctc atccagagac acctgagccc
     5221 gcgcggcctg cagttgcgcg gcggctgcat cattgccggc cttcttgtct tcccgctcct
     5281 gctcagatac cacccctttg ggcgaatcgc gccagcgcgc gtaggtggtg ctggcgaatt
     5341 cggcagtggc ctcgctgact ttgacctgcg cctgcgcggc ttgcagtttc gctttggccg
     5401 ccatcagctc tgcgtccagc tcgggagtat cgattgtggc catcaattgg ccagctttca
     5461 catgatcgcc gatgtccaca taccacttgg ccacgtagcc gtcgacccgc gcatagatcg
     5521 tgctctcgta ccacgccgcg gtctcgccgg gcagagtcaa agtgccggtg ccggtgttgt
     5581 cgcgcaccac gaccacatcg acggcgggta ccgccctcgc ggaagcctgc gactcctttg
     5641 ccagcaaatg ccgcgcatgc agtctgatga ggaactcagc tgcgaaggca atcgccagga
     5701 tcacggcgac ggcgatggcg gcgcgctgca ggcgccgccc ggtatcatta gatggcaaga
     5761 ggatctcctg tggtgcttag gccggagtga gcgaccggtc ggcgcctcaa cacccgatac
     5821 atggtcggaa caaataccag tgttgcaaag gtggcgaaca gcagtccgcc gatcaccgcg
     5881 cgcgcgagcg gggcgttctg ttcgccgccc tcgccgacgc ccagcgccat cggaatcatg
     5941 cctagcatca ttgcgcctgc agtcatcagc acggggcgca gtcgtgtgta gccggcagcg
     6001 gcggcggcca catcggaatc atccccggca tccatgcgct gattggcgaa ggtgaccacg
     6061 agaatgctgt tggcggtagt cagtccgatg cacatcagcg tgcccatcag cgccggcacg
     6121 ctcagatgcg ttcccgtcag gtacagcatc cacatcacac cgccgagcgc aaaggggacg
     6181 gccagcaata cgatcaatgg atcgagccag ctctggaaat tgatgaccag gaacagatac
     6241 acgagcatga cggcaagtgc cataccggag aacaggcccg tgtagctatc gagcatcgtc
     6301 tcgatctgtc cgctcagtgt gaccgtcatg gcactcgacg cgggtggcct gtcggccgca
     6361 atcacctgct ggatggcggc ggcggcggaa tagagatcag tgccctggat atccgcgtcg
     6421 acgtcgaata cgggccgaat attgagctgc gagagcacca gcggcgccac cccgcggttg
     6481 aagcttgcga cattcatcag cagttcacgt gagttgcggc cgtcgccgcc ggacacaggc
     6541 atggtccaca ggtcgtgcga ggaatcgatg tgataggtgg gcagctgcac gaccaatggg
     6601 tagctcacct ggttcttcgg gtcgacccag aaattcggcg cgctttgcgc gctgctgttg
     6661 gtcgtaatca gcaagtcgtt cgccgtcgag cgctgagcca tgcccagttg cgatgccatc
     6721 gagcgatcca cattgacgtc aagggtgggc gcatccggca cctgaaacac gtgtgagtcc
     6781 accacgccag gcacgtgatg cagatcgcgt accatcttgt tggccatggc gtaggccgcg
     6841 ttgctgtcgg gtccggaaat gcggatatcg atcggcgccg gctgaccgaa gttcagcacc
     6901 tgatcgacga tatctgcggg ctggaagaaa aagctcatgt ccggaaaacg ctgcggaagc
     6961 tcgcggcgaa gcttcgcgat cagctgcgcg gtcggggtgt gcttttcctt gagcgatacc
     7021 aggatttcgc catccatcgg ccccacggtt gcggaatcgc tcagcgcgat attcatgccg
     7081 ctgtagggaa gaccgatgtt gtccagaatc gtgtcgatct gcgcgttgcc tgcgatattg
     7141 cgaatcgccg cctccacctc ggcaaaatcc cgctgtgtct cctcgaggcg cgtgccagga
     7201 ggcgcgcgaa cgtgcagacg gaattgtccg gcgtccacct gtggaaagaa gtcacgaccg
     7261 agcgcaggga agagcatcag cgagcaaacg atcagtacgc cgaagaatag cgcagtgcgc
     7321 agcggttctt taagcgccca gttcagcgca tcgcgataga cggtgcggaa acgctcgaac
     7381 cggtcgatga aaccggtatg tatcgccatg aatggattgc ggcgtgcgcc ggtggcgccg
     7441 tggacgggct cggggtccgg caatgggccg gtcgccgtat gtccgcgcat cagatggaag
     7501 aacaggaccg gcaccatcgt gaacgatagg accaggctcg ccagcagcga tatgcacact
     7561 gacagcgaca gcggcgaaaa tagatatttc gcggtcccct gcagcaggaa tactgggacg
     7621 aacacgatgc agatgcacag cgtcgcgagg aaggtgggaa ttcctacctc gctggcgcca
     7681 ttgacgatcg cctccacaag aggctcccgc aggctcaggt ggcgctcgat gttctcgatg
     7741 acgaccgtgc cgttgtccac caggatgccg acggctagcg cgaagccgcc gagcgtcatc
     7801 gtgttgagcg tctggccgcc gacgtacatc accaggatag cggtcacgat tgacagtgga
     7861 atcgccgcca ga
//
LOCUS       DATMRM010000051         7190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_649739, whole genome shotgun sequence.
ACCESSION   DATMRM010000051 DATMRM010000000
VERSION     DATMRM010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649739"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VNZ06_01445"
     CDS             <1..449
                     /locus_tag="VNZ06_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWW19443.1"
                     /translation="GEWVGVALRMAGEMYLVAREEAREVMGVPTPLTRVPGAKPWILG
                     LANIRGQLYPIIDLRSYLGSGQTPTSRNSRVIIVNHRDIPAGLVVDEVLGFRRFADAE
                     FTGDVPTTIVRCERYVAGSFRRASEQWPVLGLRTLVEDPAFSEASA"
     gene            446..1867
                     /locus_tag="VNZ06_01450"
     CDS             446..1867
                     /locus_tag="VNZ06_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWW19444.1"
                     /translation="MSADSSGSAGRPQLLIISAASLIVLAAIASVALPALHVPSVLAS
                     IIALLALIPIVLLLSYLRRIGQVASQAQRAAEVQRLESDRNQQAIMRLLDEMSNLAEG
                     DLTVQATVTEDITGAIADSVNYAVEALRKLVATISQSGIQLDGAARQTQALASHLARA
                     SSAQSKQIGSATESIGAMASSIEEVSGNAERGADVARHSVDVAHKGGDAVRRTIDGMN
                     TIRETIQETSKRIKRLGESSQEIGNIVELISDIAEQTNILALNASIQASMAGEAGRGF
                     AVVADEVQRLAERAANATKQIEALVRTIQTDTNEAVVSMERSTTDVVGGALLAENAGA
                     ALQEIEQVSNQIASLVQNISASARGQSTVAQNIARNMQVLREISAQSADSTSATSQAI
                     AKLADLSASLRKSIAGFRLPGSTDATSMHRAISSSVAKQQAAIVPGASGKPSGATVAA
                     PTPAATSAPTGYSNPKVKMLGGS"
     gene            1873..>7190
                     /locus_tag="VNZ06_01455"
     CDS             1873..>7190
                     /locus_tag="VNZ06_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HWW19445.1"
                     /translation="MTELATQTLHTVAREIGVSLNEGRVALEAHVEKPGSPELLERCR
                     FELHQVQGVLRVLEIYGAALLAEEMEQVARYLIALGAERKNQDETLDALMRAMVQLPG
                     YLERVLAGGRDMALVLLPLLNDLRAVRGSALLSEGTLLLLNLKSDRQAQPAAPAPGEA
                     PLSVAQWGRRLRARFQTGLIGWIRGERTEQNLEIMGAVSTRLEQVATTQPVFQLWWVV
                     GALIESLRETGNDGSISVKRLLGLADREMKRLYDQGEARYAQHPPVELLNNLLYYIAR
                     STSVGPRVAAVRASFRLNELLPVDESIEQERENLSAPSIKLMHTVAGAIREDLSKVKD
                     VLDIYVRRGGGQAEELQPQVVLLKKIGDTLGVLGLGELRGKVQDATNRLQGMIGGTES
                     AGETALVQVAASLINVEDHLDDELVGLILPRGSKAADSADGEAPDNDFQQVQGAVLRE
                     CVINLSRIKEYVAQNVSGTLDPAGFDNWPDLMRGINAGLLMLGKSRAVEIIEDITKHL
                     KRVMQPGGTGLAPDYLDRLADAIVSLEYYIETLQAGRSDPWYMLDNARACLDALARAP
                     ERVLPTIPPVNAGTFAKTMRIPPPGSATGETTDVRKLDQPALVEPPVLTAAQTASPTA
                     ATLPDPDLIRLFIEEAREELARIEHQYPIWDQNPLESEALLVVRRSFHTLKGSGRMVN
                     ARHISEFAWAVENLLNRIIEGTLQRSPPVLETLRSAMSVMPKLVAELEPSHGGRTDVS
                     PLAARAHALASGRDTPPTPPGASAPVPPAVAAATAAPAPVPAPISAPPAVAAPTAQLS
                     VEVESRRHDDTTHLTADDRAAMAVQSSSIEIESVDLDRLLDPVKQPAEDANEPDAALR
                     EIYARETASHVGTVRRWLARERSATAPHLLAEEVYRACHTLVGSSTMAEARHGIRLAE
                     PMNHWLRKSFDSGIGLDDSDLALIADCMAAMETVASHLDEGTGFFLVHDTLRARIARA
                     EIELDRRISEATALAGRTSTHLSAPPIETRAPLPAVPLAAAPATPPVAVTAPPPPAAA
                     VEAASPDFDPEIASIFSEEAVELLEACESAMSAWNAAPQDREQVAALRRPLHTLKGGA
                     RMAGITAMGELAHELETLITRIESGLTGTDDNARGVAQEALDELSRMRESIASGRAVS
                     RQSALVERIHAVSSGTALPGSTVPAAAAPPPPAVPIQAPPQALRPSPVAAAATPTSPY
                     ALAAMPVASPVAAPEPIQIPELSMLPKEEELAPRAEVPQARAALAMPPQVSAPARMEA
                     TPEPPRLSLVQSEPALRPPPPRAEVSHDEFVAAESAEAVASAELPAAPALLPPGREPV
                     VAAERVEMARVSAELLDLLLNNAGEVSIARARLEQQLGAIEFNLGELSRTVTRLKDQL
                     RKLEIETEAQILHRHDNETAHRQDFDPLELDRYSSIQQFSRALAESSNDVGSIQGLLE
                     NLIAEAQNLLQQQSRTVTELQNGLMRTRMVSLQRHVQRLARIVRQAGADSGKRAELIV
                     DGASGELDRQVLERMLPPFEHMLRNAVVHGIETPDVRRAAGKDETGRIHLNLKREGAE
                     VIVEVSDDGDGMNLTAIRVKGLEMGLIRADQELSDEDIMQLVLEPGFSTASSLTQQAG
                     RGVGMDVVAVEIKKLGGALHMESSRGRGTRFTIRLPFTLAISHALVVRTGEEFYALPL
                     PTVEGVVRLARAEVLQHLRSETASFTHSGIRYRLQPLALYVGMEASALPEQDVTIPVV
                     LVRAGEHSTGLIADELVGSREIVVKPVGPQISSIRGISGATILGDGR"
BASE COUNT         1288 a         2346 c         2387 g         1169 t
ORIGIN      
        1 cgggcgaatg ggtaggggtg gcgcttcgga tggcagggga gatgtacttg gtggcgcgcg
       61 aagaggcgcg cgaggtcatg ggcgttccga cgccgctgac gcgcgttccg ggcgccaagc
      121 cgtggattct gggtctggcc aatatccgcg gccagctcta tcccatcatc gatctgcgct
      181 cctacctcgg cagtggccag acgccgactt cacgcaactc gagggtcatc atcgtcaatc
      241 atcgtgatat tcctgccggg ctggtcgtcg atgaggtgct cggcttccgg cgctttgccg
      301 acgccgaatt caccggcgac gtgccgacga cgatcgtgcg ctgcgagcgc tatgtggccg
      361 gctcgttccg tcgcgccagc gagcaatggc cggtgctggg cctgcgcacc ctggtggaag
      421 atcctgcctt ttccgaggcc agcgcatgag cgcggacagt tccggtagcg caggccgtcc
      481 gcaactgctg atcatcagcg ccgccagtct gatcgtgctg gcggccatcg ccagtgtggc
      541 gctgccagcg ctgcatgtac cgtcggtgct ggcatcgatc atcgccctgt tggccctgat
      601 ccccatcgtg ctgctgctgt cctacctgcg ccgcatcggc caggtcgcat cccaggcgca
      661 gcgcgccgct gaagtccagc gcctggagag cgaccgcaat cagcaggcca tcatgcgact
      721 gctggacgag atgtcgaatc tggccgaggg cgatctgacg gtccaggcga ccgtcacgga
      781 agacatcacc ggcgcgatcg ccgactcggt gaattacgcg gtcgaggccc tgcgcaagct
      841 ggtggcgacg atcagccagt ccggcatcca gctggacggc gccgcccggc agacccaggc
      901 gctcgcctcg cacctggctc gcgccagcag cgcccagtcc aagcagatcg gttccgcgac
      961 cgaatccatt ggcgcgatgg cctcatcgat cgaggaagtg tccggcaacg ctgagcgtgg
     1021 tgcggacgtc gcccgccatt ccgtggacgt cgcccacaag ggcggcgatg ccgtacgccg
     1081 cacgatcgat ggcatgaata ccatccgcga gaccatccag gagaccagca agcgcatcaa
     1141 gcggctggga gagagctcgc aggaaatcgg caatatcgtc gagctgatca gcgacattgc
     1201 tgagcaaacc aacatcctcg cgctcaatgc ctcgattcag gcttcgatgg cgggagaggc
     1261 cgggcgcggt tttgcggtcg tggcggacga ggtccagcgt ctggccgaac gcgccgccaa
     1321 cgcgaccaag caaatcgaag cgctggtgcg tacgatccag accgatacca atgaagcggt
     1381 ggtgtcgatg gagcgcagta ccaccgacgt cgtcggtggc gccctgctgg cggaaaacgc
     1441 cggcgctgca ctgcaggaaa tcgagcaggt gtcgaatcag attgccagcc tggtacagaa
     1501 tatctccgcg agcgcgcgcg ggcagtccac cgttgcccag aacatcgcgc gcaacatgca
     1561 ggtgctacgc gaaatcagtg cccagtccgc cgattccacc agtgccacgt cgcaggcgat
     1621 cgccaagctc gccgatctgt ccgcgtcgct gcgcaaatcg atcgccggat tccgtttgcc
     1681 tggcagtacc gatgccacca gcatgcaccg cgccatctcg agcagcgtcg ccaaacagca
     1741 ggcggccatc gtgccaggcg cgagcggtaa gccgagcggt gcgaccgtgg ccgcgccgac
     1801 gccggccgcg acatcggcgc ctaccggcta ttccaatccg aaggttaaga tgcttggcgg
     1861 ctcctagaac gcatgactga gctcgcaact caaaccttac atacggtcgc acgcgaaatc
     1921 ggcgtgtccc tgaatgaggg gcgtgtcgcg ctggaagcgc acgtcgaaaa accggggagc
     1981 cccgagctgc tggagcgctg tcgtttcgaa ctgcatcagg tccagggtgt gctgcgcgtg
     2041 ctcgagatct acggcgccgc gctgctggcc gaggaaatgg agcaggttgc ccgctacctc
     2101 atcgctctgg gcgccgaacg caagaatcag gatgaaaccc tcgacgcgct gatgcgcgcg
     2161 atggtgcagc tgcccgggta tctggagcgc gtactggccg gcggccgcga catggcgctg
     2221 gtgctgctgc cgctgctcaa cgacctgcgc gcggttcgtg gtagcgcctt gctgtccgaa
     2281 ggcacgctgc tgttgctgaa tctcaagtca gatcgtcagg cgcaacccgc ggctcccgcg
     2341 ccgggcgaag cgccgctgag cgtggcgcag tggggccggc gcctgcgcgc ccgcttccag
     2401 accggcctca tcggctggat ccgcggcgag cgcaccgaac agaatcttga aatcatgggt
     2461 gcagtgtcta cccgcctcga acaggttgcc acgacgcaac cggtatttca gttgtggtgg
     2521 gtggtcggtg cgctgatcga atcgctgcgc gagaccggca atgacggcag catttccgtc
     2581 aagcgcctgc tgggattggc cgaccgcgag atgaagcgcc tctacgatca gggcgaggcg
     2641 cgctacgcgc agcacccgcc cgtggagcta ctcaacaatc tgctgtacta cattgcgcgc
     2701 tcgaccagcg tcgggccccg tgtggcggcg gtgcgtgcgt cgttccgtct gaacgagctg
     2761 ctgccggtcg acgagtcgat cgaacaggaa cgcgaaaacc tctcggcgcc ctcgatcaag
     2821 ctgatgcaca ccgtggctgg tgcgatccgc gaggatctgt ccaaggtcaa ggatgtgctg
     2881 gacatctacg tgcggcgcgg cggcggccag gccgaagagc tgcagcccca ggtcgtgctg
     2941 ctgaagaaga tcggcgacac gctcggggta ctgggtctgg gcgagctgcg cggcaaggtg
     3001 caggacgcga ccaacaggct gcaggggatg attggcggca ctgagagcgc tggtgagacc
     3061 gcgctggtgc aggtcgccgc cagtctgatc aatgtggaag atcatctcga cgatgaactg
     3121 gtgggcctga tcctgccgcg cggtagcaag gccgcggaca gcgccgacgg cgaagcgccg
     3181 gacaatgact ttcagcaggt gcagggcgcg gttttgcgcg aatgcgtgat caacctgtca
     3241 cgcatcaagg aatacgtcgc gcagaacgtc agcggcacgc tggatccggc cggcttcgac
     3301 aactggcctg atctgatgcg cggcatcaac gctggcctgc tgatgctcgg caagtcgcgt
     3361 gcggtcgaga tcatcgagga catcaccaaa catctgaagc gggtcatgca gcccggcggc
     3421 accggcctgg cccctgatta cctggaccgc ctggccgatg cgatcgtcag cctcgagtac
     3481 tacatcgaga cactacaggc gggccgcagc gatccctggt acatgctcga taacgcccgc
     3541 gcgtgcctgg acgcgctggc gcgtgcgccg gagcgggtac tgccaaccat tcctccggtg
     3601 aacgcgggta cgttcgccaa gacgatgcgc attccgccgc ccggatcggc caccggagag
     3661 acgaccgatg tgcgcaagct cgatcagcct gcgctggttg agccgccggt actgaccgcc
     3721 gcgcagacgg cgtctccgac ggccgccacg ttgcccgatc ctgacctgat acgcctgttc
     3781 atcgaggaag cgcgcgaaga gctggccagg atcgagcatc agtatccgat ctgggaccag
     3841 aatccgctcg aaagcgaagc gttgctggtt gtacgacgct cgttccacac cctcaagggc
     3901 agcggccgga tggtcaatgc ccgccatatc agcgaattcg cctgggcggt cgaaaacctg
     3961 ctcaatcgca tcattgaagg taccttgcaa cgctcgccac cagtgctgga gacattgcgt
     4021 tcggcgatga gtgtgatgcc gaagctcgtg gccgagctcg agcccagcca tggcgggcgt
     4081 accgacgtct cgccactcgc ggcccgtgcg catgcgctgg ccagtggtcg ggacactccg
     4141 ccgacgcccc ccggcgcgtc cgcacccgtg cctcccgcgg ttgctgcggc aacagcggcg
     4201 ccggcaccgg tacccgcgcc gatcagcgcg ccgcccgcag tcgctgcgcc gaccgcccag
     4261 ttgagcgtcg aggtcgaatc gcgtcgtcac gacgacacca ctcatttaac cgctgacgac
     4321 cgtgcagcga tggcggtcca gagctcatcg atcgagatcg agagcgtgga tctggatcgt
     4381 ctgctcgatc cggtcaaaca gcctgcggaa gatgccaacg aacccgacgc cgcgctgcga
     4441 gagatctacg cccgcgaaac ggcgagtcat gtcgggacgg tgcgccgttg gctggcgcgc
     4501 gagcgatcgg ccactgcgcc acacctgctg gccgaagagg tgtatcgcgc gtgccatacg
     4561 ctggtcggca gttccacgat ggcggaggcg cgccatggca tacgcctggc cgagcccatg
     4621 aatcattggt tgcgcaagtc attcgacagc ggcatcggtc tggatgattc ggatctggcg
     4681 ctcatcgctg actgcatggc cgcgatggaa accgtggcca gccatctgga cgagggtacg
     4741 ggattctttc tcgtgcatga cacgctgcgc gcccggattg cgcgcgcgga gatcgaactg
     4801 gatcggcgca tctcggaggc caccgcgctg gccggtcgta ccagcaccca tctgagcgcc
     4861 ccgccgatcg agacgcgcgc accgctaccc gccgtaccgc tcgcagcggc gcccgcaacg
     4921 ccgccggtcg cagtcacggc gccaccaccg cctgcggcag ccgttgaagc ggcatccccg
     4981 gatttcgatc ccgagatcgc atcgatcttc agcgaggagg ccgtcgaact gctcgaggcc
     5041 tgtgaatcgg cgatgtcggc gtggaatgcc gctccccagg atcgcgaaca ggttgcagcg
     5101 cttcgacggc cgctgcacac cctgaagggt ggagcgcgca tggccggcat cacggcgatg
     5161 ggcgagctgg cgcatgaact ggaaaccctg atcacgcgca tcgagagtgg tctgacaggc
     5221 acggatgaca acgcgcgcgg tgttgcgcag gaagcgctcg atgagctgtc ccgcatgcgc
     5281 gagtcgatcg cgtcgggtcg cgcagtgtcg cgccaatcgg cattggtcga acgaattcat
     5341 gccgtctcga gcggtaccgc gctcccaggg tccaccgtgc ccgctgcggc ggcaccacca
     5401 ccaccggcgg tcccgataca ggccccgcca caggccctgc ggccttcacc cgtggcggcc
     5461 gccgcgaccc cgacatcacc ctatgcttta gcggcgatgc ccgttgcgtc gccggtggcg
     5521 gcgcccgagc cgattcagat ccccgaactg tcgatgttgc cgaaggaaga ggagctggcg
     5581 ccgcgtgccg aggtgccgca ggcccgggcc gcgctcgcca tgccgccgca ggtcagcgcc
     5641 ccggcgcgaa tggaggccac ccccgagcca ccgcggctct cgctggttca gtccgaaccc
     5701 gcactccggc cgccgccacc gcgtgcagaa gtcagtcacg atgaatttgt cgccgcggaa
     5761 tccgccgaag cggtcgccag cgccgagttg ccggccgctc ccgcgctgtt acctccgggt
     5821 cgggaaccgg tggtggccgc cgaacgcgtc gagatggctc gagtcagcgc ggagctgctc
     5881 gatctgttgc tgaacaacgc cggtgaggtg agcattgcgc gcgcgcgcct cgagcagcag
     5941 ctcggcgcga tcgaattcaa cctgggcgag ctgtcgcgca cggtgacgcg actgaaagat
     6001 cagctgcgca aactggaaat cgagaccgaa gcgcagattc tgcaccgaca cgacaacgag
     6061 accgcgcatc gtcaggactt cgatccgctg gaactggacc gctattcatc gatccagcag
     6121 ttttcgcgcg cgctggcgga atcctccaac gacgtcggca gcattcaggg cctgctcgag
     6181 aacctgatcg ctgaagcgca gaacctgcta cagcagcagt cgcgcaccgt cacggaactg
     6241 cagaatggcc tgatgcgcac tcgcatggtg tcgctgcaac ggcacgtgca gcgtctggca
     6301 cgcatcgtgc gacaggcggg ggccgatagc ggtaagcgcg ccgagttgat cgtcgatggc
     6361 gcttccggcg aactggaccg acaggtgctc gaacgcatgt tgccgccgtt cgagcacatg
     6421 ctgcgcaacg cggtggtcca cggcatcgag accccggacg tgcgccgcgc cgcgggtaag
     6481 gatgaaaccg gacgcatcca cctgaacctc aagcgcgagg gcgcagaggt gatcgtggag
     6541 gtcagcgacg atggcgacgg gatgaatctg acggcgatcc gggtcaaagg actcgagatg
     6601 ggattgatcc gcgccgatca ggagctgtcg gacgaggaca tcatgcagct cgtgctcgag
     6661 ccgggcttct cgaccgccag ttcgctgacc cagcaggccg gccgcggcgt cggcatggat
     6721 gtggtcgccg ttgaaatcaa gaaactcggc ggcgccctgc acatggagag ctccaggggc
     6781 cggggcacgc gtttcacgat tcgcctgccc ttcacgcttg ccatcagcca tgcgctggtg
     6841 gtgcgcacgg gcgaggagtt ctacgcgctg ccgctgccaa cggtcgaagg cgtggtacgt
     6901 ctggcacgcg cagaagtcct gcagcatctg cgcagtgaaa cggcgagctt cacccactcc
     6961 ggtatccgct atcgcctgca gccgctggcg ctgtatgtcg gaatggaagc ctcagccttg
     7021 ccggagcagg acgtgactat tccggtggtg ctggtgcgcg ccggtgaaca ttcgaccggg
     7081 ttgattgccg atgaactggt cggcagtcgc gagatcgtcg tcaagccggt aggacctcag
     7141 atttccagca ttcgaggcat ctccggcgcc accatcctgg gcgacggccg
//
LOCUS       DATMRM010000052         6905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_336674, whole genome shotgun sequence.
ACCESSION   DATMRM010000052 DATMRM010000000
VERSION     DATMRM010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336674"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VNZ06_01460"
     CDS             <1..546
                     /locus_tag="VNZ06_01460"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HWW19446.1"
                     /translation="LARYAAICQEQNIVPIVEPEVLMDGGHKIERCEEVTDEVLAEVF
                     SQLEDHRIHLEGMILKPNMVISGKKCSTQASPELVAAATVRCLKRHVPSAVPGIMFLS
                     GGQSAAEATLHLSLMNKLGPLPWQLSFSYGRALQDAALSAWGGKPSNVGAGQKEFHKW
                     SKLNGLARTGAFKAGMEQQAA"
     gene            543..1355
                     /locus_tag="VNZ06_01465"
     CDS             543..1355
                     /locus_tag="VNZ06_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004809856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWW19447.1"
                     /translation="MSDSDSVVEVSDVHYHIGARAIFNGVNMRMRRGAITAVMGPSGT
                     GKTTLLRLIMGQILADSGKVTVFGQDVAALNRSHLFALRQRIGMLFQNGALLTDMSVF
                     ENVAFPVREHSNLPEPVLRELVLMKLQAVGLRGAADLMPAELSGGMARRVALARAIVM
                     DPEVLIYDEPFVGLDPISMGVIVRLIQSLNQTLGITSILVSHDVAEISAIADYTYLLS
                     EGQVVAEGSPKELKAEQKEVVKQFMSGAADGPVPFHYPAPDYFGQLLERTSA"
     gene            1352..2134
                     /gene="mlaE"
                     /locus_tag="VNZ06_01470"
     CDS             1352..2134
                     /gene="mlaE"
                     /locus_tag="VNZ06_01470"
                     /inference="COORDINATES: protein motif:HMM:NF033619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HWW19448.1"
                     /translation="MKIHSAAVTINRRSLALGALAKVGSVALFFFRIVGQSVPALGRP
                     ALVVKQIYNAGARSLVVIMLSGLFVGGVLGILGDDLLQRFGSESALGVVAALGLTKEL
                     GPVLTALLFAGRAGTALCSEIGLMKATDQLAAMEMMAVDPIRHVVAPRFLGGLIAMPL
                     LAAIFNCIGIYGAQLVGISMMGIQPGTFWSQTQDAVGIRDVNEGLIKSVVFGAACSLL
                     AVYEGYHAEPTAEGVGLATTRTVVTSAVLVLLLDYILTAFFL"
     gene            2162..2743
                     /gene="mlaD"
                     /locus_tag="VNZ06_01475"
     CDS             2162..2743
                     /gene="mlaD"
                     /locus_tag="VNZ06_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HWW19449.1"
                     /translation="MAQSNRSIEVGTGLFVLLGFASLGFLTTQLPGSGLQLTRTADSY
                     AVVAKFDNIGSLKVGAPVEMSGVRVGSVTAINYDASVYKAAVNMAIDRKYDKIPDDSD
                     AAIQTSGLLGANYIAVGPGGSDTYLKAGSEMTFTQSAIVLENLINKLFSSVASGGNKD
                     SDSGSKDSGSKDSGSKDEGSKDSGKPSGGKSSK"
     gene            2751..3464
                     /locus_tag="VNZ06_01480"
     CDS             2751..3464
                     /locus_tag="VNZ06_01480"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWW19450.1"
                     /translation="MRNAKFALVAALSLGALGALAVLPANAAQSSPAPATGTGGNLGA
                     GVDSSAPAKLVQTAASAMLKELDAHRADYRANPGKVHGLVDQVLLPHFDTTYSARLVL
                     GRHWRDANDDQRKRFVDAFYRSLLNNYGDALVDFTADKLQVLPYNGDPKADNATVKTK
                     VRKSDGSSVEVDYALHKTDDGWKAWDVIIEGISYVRSFREDFDSEIEQKGIDEVITRL
                     QSGETPQAIKHTTTTLKKS"
     gene            3470..3775
                     /locus_tag="VNZ06_01485"
     CDS             3470..3775
                     /locus_tag="VNZ06_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWW19451.1"
                     /translation="MAAASAFVAVSSGLYRLEAPLTFASVAGLRDQGLKMIDSAPEGL
                     TLDLQAVPTVDSAGLALLIDWLGEARSRSRRLKYVQPPNALLSLARLSEVERLIAEG"
     gene            3787..4413
                     /locus_tag="VNZ06_01490"
     CDS             3787..4413
                     /locus_tag="VNZ06_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19452.1"
                     /translation="MNQQTSETEPGLRKALDHLQSVLIPGENLEAWAVQRRIYAIKHR
                     RLLVAATSGRLICIQRNLLGGFQLHDIRWQDLEEVRLRVGVLAADLTVRVGSASDLAS
                     QGAGGARTMEFHGLRTPQAEAVYRICQAQDQAWREKRRIRELEELRARAGGIQLGAGS
                     AASTAAAPAADGVPSEAMRRLQEAKQMLDAKLITDSEYEAIKARIVSS"
     gene            complement(4417..5181)
                     /locus_tag="VNZ06_01495"
     CDS             complement(4417..5181)
                     /locus_tag="VNZ06_01495"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HWW19453.1"
                     /translation="MNRAALLALLLLNLGITGCAALPNGKHDPRDRFERFNRSVYKFN
                     TALDHAVLRPVARAYVRYVPGPIRTSLSNFTSNLAYPTTVANDVFQGKITDGVSDAAR
                     IIVNTTIGIGGLFDPATRMGLDRHTQDFGLTLGRWGVPSGPYLMLPLLGPSTVRDTAG
                     LGPDYLLTYEIVSRNLITNNYVSGGLFVVTQIDRRAGLLDTDKALDAAYDPYGIVRSA
                     YLQRRNYLLRGDETPDDLLKEDESDNGGKDKPDPPK"
     gene            complement(5252..6775)
                     /locus_tag="VNZ06_01500"
     CDS             complement(5252..6775)
                     /locus_tag="VNZ06_01500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HWW19454.1"
                     /translation="MRRICALFGCFLTLSASYSAVAGDPPVVVPTTATPASFSTRSNP
                     YLGYDLPDLGGPANALISKADEYSIGRMVLREVRDSGLLMEDPEVTDYIQQLGMRLAS
                     QAHDEGQSFTYNVMKEPVVNAGAIGRLVLINSGLILMTDNESELASVVAHETGHIVQR
                     HMARAIEAQSHMSLINMAAMLAAIALGAMAGGGQGAEGAIALTQAASYQQQVNLIRGN
                     EIEADAVGIELLAGAGFDPNEMANEFELFSRMEGLSLAGIPPLLIDHPVTSERVAAAR
                     NRAATFPPSKPLPESLSYELFKERVRVLALPPEEHVDQYYSRLRDRRPLSAAERYGEA
                     LVQMQNHNAAAAVAILRELVAQNPQVTMLYATLGQALVANNQMPEALEFFRQTNILFP
                     RNVPLTVRYAETLMQANRAPEAHTLLLDLFNNADPTPAQIRLTALAANAAGDAGDAYE
                     YMAEYDLVGGQLALANQQLELALATPGLTGQQKAKFRARLEQVRGWLREQQASRRTG"
BASE COUNT         1263 a         1990 c         2291 g         1361 t
ORIGIN      
        1 ctggcacgtt acgcggccat ttgccaggaa cagaacatcg taccgatcgt cgaacccgaa
       61 gtcctgatgg atggcggaca caagattgag cgctgtgaag aagtgaccga tgaggttctg
      121 gcggaggtct tctcgcagct cgaagatcat cgcatccacc tcgagggcat gatcctgaag
      181 cccaatatgg tgatctccgg caagaagtgc agcacgcagg catcgcctga actggtcgct
      241 gccgccacgg tgcgttgcct taagcgacac gtgccctcgg cggtgccagg catcatgttc
      301 ctgtcgggcg ggcagtcggc ggctgaggcc acgctgcatc tgtcgctgat gaataagttg
      361 gggccgctac cgtggcagct gtccttcagc tacggccgtg cgttgcagga tgccgcgctc
      421 tcggcctggg gcggcaagcc ctccaacgtc ggcgcggggc agaaggaatt tcataagtgg
      481 tcgaagctca acgggcttgc gcgcaccggc gctttcaagg ccggaatgga gcagcaggca
      541 gcgtgagtga ttctgattcg gtcgtcgagg tcagcgacgt tcactaccac atcggcgcac
      601 gcgcaatatt caacggcgtg aatatgcgca tgcgccgcgg agccatcacg gcggtgatgg
      661 gacccagcgg caccggcaag accacgctgt tgcgtctgat catggggcag atcctggccg
      721 acagcgggaa agtgacggtg ttcggacagg acgtcgcggc tctgaatcgc agtcacctgt
      781 tcgctcttcg ccagcgcatc gggatgctgt ttcagaacgg cgcgctgctg accgacatgt
      841 cggttttcga gaacgtagcc tttccggtgc gtgaacattc caatctgccc gagccagtgc
      901 tgcgcgagct ggtgctgatg aaactgcagg cggtcggttt gcgcggagcc gccgatctga
      961 tgccggctga gctctccggc ggcatggcac ggcgtgtggc gctggcacgc gcgatcgtga
     1021 tggacccgga ggtgttgatc tacgatgagc ctttcgtggg tctggatccg atttcgatgg
     1081 gtgtgattgt gcgcctgatc cagtcgctca atcagacgct ggggatcacc agtatcctgg
     1141 tgtcgcacga tgtggccgaa atcagtgcga ttgctgacta cacctacctg ctgtcggaag
     1201 gccaggtggt ggccgagggg agtccgaagg aactgaaggc ggagcagaag gaggtcgtca
     1261 aacagttcat gagcggggcc gccgacgggc cggtgccgtt ccactacccg gcgccagact
     1321 attttggtca gttgctggag cgcacctcgg catgaagatt cacagcgcgg cagtgacgat
     1381 caaccggcgt tcattggcac tcggcgcgct ggcgaaggtc gggtcggtgg cactgttttt
     1441 cttccgaatc gtcgggcaga gcgtgccggc gctggggcgc ccggctctgg tggtcaaaca
     1501 gatctacaac gccggtgccc gctccctggt cgtcatcatg ttgtcgggtt tgttcgttgg
     1561 cggcgtgctc ggcatactgg gcgacgacct gttgcagcga ttcggttctg aatcggcgct
     1621 gggcgtggtg gccgccttgg ggttgaccaa ggaactcggt ccggtgctga cggcgctgct
     1681 ttttgccgga agggcaggta ccgcgctgtg ttccgagatc ggcctgatga aggccaccga
     1741 tcagcttgcg gcgatggaga tgatggccgt cgatccgata cgtcacgtgg tcgcgccacg
     1801 ctttctggga ggtctgatcg cgatgccgct gttggcggcc atcttcaact gcattggcat
     1861 ctatggtgcg cagttggtcg gcatctcgat gatgggaatt cagccgggta ccttctggtc
     1921 gcagacgcag gacgccgtgg gaattcgcga cgtcaacgag gggctgatca agagcgtcgt
     1981 tttcggtgca gcctgcagtc tgttggcggt gtacgagggc tatcacgcgg agccgacggc
     2041 ggaaggggtg ggtctggcga cgacgcgaac ggtggttact tcggccgtat tggtgctgtt
     2101 gcttgactat atcttgaccg ctttcttcct gtagggcctc ttttgaaagg ggattgcttt
     2161 catggcacag tcgaatcgtt caattgaagt cggaaccggg ttgtttgtgc ttctggggtt
     2221 tgcttccctc ggatttctga ctacacaatt gcccggcagc ggcttacagc tgacgcggac
     2281 ggctgatagc tacgctgtgg tcgcgaagtt cgacaacatt ggctcactga aagtcggtgc
     2341 gccggtggag atgtcgggtg tgcgtgtcgg ctcggtcact gccatcaact acgacgcctc
     2401 ggtctacaag gccgcggtga acatggcaat tgaccgcaag tacgacaaga tccccgatga
     2461 cagcgatgcc gccattcaga cctccggact gctcggcgcc aattacattg cagtggggcc
     2521 agggggttcg gacacctatt tgaaagctgg cagcgagatg actttcacac agtcagcgat
     2581 cgtgctcgag aatctgatca acaagctgtt ctccagcgtc gccagcggtg gcaacaagga
     2641 ctccgacagt ggcagcaagg atagcggcag taaggatagc ggcagtaagg acgagggcag
     2701 caaggacagc ggcaagccat ccggcggcaa atcatccaag tgagggcatc atgcgaaacg
     2761 cgaaatttgc actcgttgca gcgctgagct taggcgcgct cggtgcgctg gccgtgttgc
     2821 cggcgaatgc cgcgcagtcc agtccggcgc cagccaccgg caccggtggc aatcttggtg
     2881 cgggggtgga ttccagcgcg cctgcgaaac tcgtgcagac agcggcctcg gcgatgctga
     2941 aggagctgga tgcgcatcgg gctgactatc gcgccaatcc aggcaaggtg cacggactgg
     3001 tggaccaggt gctgctgccg cacttcgata ccacttattc ggcccggctg gttctgggaa
     3061 gacactggcg cgacgccaac gacgatcagc gcaagcgctt cgtggatgcg ttctatcgct
     3121 cgctgctcaa caactatggc gatgcgctag tggacttcac ggccgataaa ctgcaggtgc
     3181 tgccgtacaa cggcgatccg aaggccgaca acgccacggt caagaccaag gttcgcaaga
     3241 gcgacggttc atcggttgag gtcgactacg cgcttcacaa gacggacgac ggctggaagg
     3301 cctgggacgt gatcatcgaa ggcatcagct atgtgcgcag cttccgcgaa gacttcgatt
     3361 cggagattga acagaagggc atcgacgaag tcatcacgcg tctgcagtca ggcgagacgc
     3421 cgcaggcgat caagcacacg acgaccacgt tgaagaagag ctgaaggccg tggcggcagc
     3481 gagcgcattt gttgcggtct cctcgggcct ctaccggctc gaggcgccgc tgacctttgc
     3541 cagtgttgcc ggcctgcgtg atcagggtct gaagatgatc gattcggctc cggaagggtt
     3601 gacgctggat ctgcaggccg tgcccacggt ggacagcgcc ggtctggcgc tactgatcga
     3661 ctggctaggt gaggcgcgct ccagatcgcg ccgcctcaag tacgtgcaac cgccgaacgc
     3721 gctgctatcg ctggcgcgcc tgagtgaggt tgagaggctg atcgccgaag gctgattggg
     3781 gctgccatga accagcaaac ctccgagacc gagccaggac tgcgcaaggc cctggatcat
     3841 ctgcagtcgg tgctcatccc gggcgagaat ctcgaggcct gggcggttca gcgccgcatc
     3901 tatgcgatca aacatcgaag actgctggtc gccgcaacca gcggccgatt gatctgcatc
     3961 cagcgcaatc tgctcggtgg cttccagttg cacgatatcc gttggcagga tctggaggag
     4021 gtccgactgc gcgtcggcgt gctcgccgcc gatctgacgg tgcgcgtcgg cagtgcgagc
     4081 gatctggcct cgcagggagc gggtggcgcc cgtaccatgg agtttcatgg cctgcgcacc
     4141 ccccaggcag aggcggtcta tcgcatctgc caggcgcagg atcaggcctg gcgtgagaag
     4201 cggcgtattc gcgagctgga agagctgcgc gcgcgcgccg gcggtattca gctcggcgcg
     4261 gggagtgcgg cgagtacggc cgcggcgcca gcggctgacg gggtcccgtc cgaggcgatg
     4321 cgtcgtctgc aggaagccaa gcagatgctg gacgcgaaac tgattaccga ttccgaatac
     4381 gaggccatca aggcgcgcat cgtctccagc tgagcgctat ttcggcgggt ccggcttgtc
     4441 cttgccgccg ttgtcgcttt catcttcctt gagcagatcg tctggagttt cgtcaccgcg
     4501 cagcaggtag ttgcggcgct gcagataggc gctgcgcacg atcccgtacg gatcgtaagc
     4561 ggcgtccagg gccttgtcgg tatcgagcag gcccgctcga cgatcgatct gagtgaccac
     4621 gaacaatcca cccgacacgt agttgttggt gatcaggttg cgactgacga tctcgtaggt
     4681 cagcaaatag tccggcccca gacctgcggt atcgcgcacc gtggacggtc ccagcagcgg
     4741 caacatcaga tacggtccgc tcggtacgcc ccagcggccg agggtcagtc cgaaatcctg
     4801 ggtatgtcga tccagtccca tgcgagtcgc cggatcgaac agtccgccaa taccgatggt
     4861 ggtattgacg atgatgcgcg cggcgtcgct gaccccgtcg gtgatcttgc cctggaacac
     4921 atcgttggcg accgtggtcg ggtaggcgag gttgctggtg aaattgctga ggctggtgcg
     4981 tatcgggccc ggcacatatc tgacgtaggc gcgcgcgacc gggcgcagta ctgcgtggtc
     5041 tagcgcagtg ttgaacttat agaccgaccg attgaaacgc tcgaagcgat cgcgcggatc
     5101 gtgtttgccg ttgggtagtg ctgcgcagcc ggtgattccg agattgagca gcagcaatgc
     5161 aagcagggca gctcggttca tggagccgca gtgtatcagg tcatccgcgg aatctgatgc
     5221 ggcgcagaag ttactgccga tttgctgctg atcagcctgt gcgccgcgag gcttgctgct
     5281 cgcgcagcca gccgcgcacc tgctccagcc gtgcacggaa cttcgctttc tgctgaccgg
     5341 tgagtcccgg tgtcgccagc gccagttcca gttgctggtt ggcgagcgcc agttggccgc
     5401 ctacgaggtc gtattcggcc atgtactcgt acgcatcccc ggcatcgccc gcagcgttgg
     5461 ccgcgagcgc ggtcaggcga atctgtgccg gcgtcggatc agcattgttg aacaggtcga
     5521 gcagcagggt gtgtgcttcc ggcgcgcgat tggcctgcat gagcgtttcc gcatagcgca
     5581 cggtcagcgg cacgttgcgc gggaacagga tgttggtttg ccggaagaat tccagtgcct
     5641 ccggcatctg gttattggca accagcgcct gaccgagtgt cgcatacagc atcgtcacct
     5701 gtggattttg ggcgaccagc tcgcgcaaga tcgcgaccgc tgcggcggca ttgtggttct
     5761 gcatctgcac cagtgcttcg ccgtaacgct ctgccgctga gagtgggcgc cggtcgcgga
     5821 gacgcgagta gtactgatcg acatgctcct ccgggggcaa cgccaacacc cgcacccgct
     5881 ccttgaacag ttcgtagctc agcgactccg gcaggggttt gctcgggggg aacgtggcgg
     5941 cgcgattgcg cgcggcggcg acgcgctcac tggtcaccgg gtgatcgatc agcagtggcg
     6001 gaatgccggc aagggacagg ccttccatgc gagagaacag ttcgaattcg ttcgccattt
     6061 cgtttggatc gaagccagcg ccagcgagca attcaattcc gacggcgtca gcttcgatct
     6121 cgttgccacg tatgaggttc acttgctgct gataggaggc ggcttgggtc agcgcgatgg
     6181 caccttccgc accctgaccg cccccggcca tcgccccgag cgcgattgcc gccagcatgg
     6241 ccgccatgtt gattagggac atgtgcgact gcgcctcgat cgcgcgcgcc atatgccgct
     6301 gcacgatgtg accggtctcg tgggcgacga cggaggccaa ctcggactca ttgtcggtca
     6361 tcaggatcag accactattg atgagtacga gacggccgat ggcgccggcg ttgacgaccg
     6421 gctccttcat cacgttgtag gtaaagctct ggccctcatc gtgcgcctgc gaagcgagcc
     6481 gcatgccaag ctgctggatg taatcggtga cttccggatc ctccatcagc agcccgctgt
     6541 cgcgcacctc gcgcagtacc atccggccaa tggagtattc gtcggccttg ctgatcagcg
     6601 cgttcgccgg tccgcccaga tcaggcaggt cgtaccccag gtagggattg ctgcgagtgc
     6661 taaagctggc cggggtggca gtggtgggca ccacaaccgg tgggtcgcca gccaccgcgc
     6721 tataggaggc tgacagcgtc aggaagcagc caaacaacgc gcaaatacgt cgcataacac
     6781 cttgagtaga caagcaatcc tacttcgacc ttggggcatg agtttcggtt ccgcccacgg
     6841 gtgggtcgcg tgaccgctat cttaatccct gggccccagg agcctataac gcttcagagg
     6901 cgaaa
//
LOCUS       DATMRM010000053         3805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3432946, whole genome shotgun sequence.
ACCESSION   DATMRM010000053 DATMRM010000000
VERSION     DATMRM010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3432946"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VNZ06_01505"
     CDS             complement(<1..628)
                     /locus_tag="VNZ06_01505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW19455.1"
                     /translation="MGSAALPDSKVGFKEFVALVGMLMATQAIAIDAMLPALPQIGAT
                     LHVANPNHTQLIVTAYVTGMGIGQLFWGLLSDRYGRRPILLTGLTVYVIAAVLSGLAT
                     SFVALLLLRLVHGMAAASVVVARSAVRDQFSGRQLARVMSLTMILFLMIPVIAPTLGQ
                     LVLAVAPWRSIFLLFGAYALIVFLWVLLRMPETLHPEYRLTLNRAQITR"
     gene            complement(721..1599)
                     /locus_tag="VNZ06_01510"
     CDS             complement(721..1599)
                     /locus_tag="VNZ06_01510"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HWW19456.1"
                     /translation="MSAPVQREASTDAFEFVRELATELTTGDIELPSFPEVAAQVQKV
                     LSQDDSDSARVVRVLGAEPMLATRVLSMANAAALNPGGKSVSDLRTAVTRLGFDSLRS
                     AAIGFAMAQIQRAKQFKGIERHLSALWQQSVLVAALAFVVARRTDKASPDTAMLTGLV
                     HGVGKLYILTHSTKHPALFGEQEMYQRIVHDWHGNIAKALLEGWYMAEEIVIAVHSFE
                     DQSRELRGVAGALADVLDVADMLSACKDDPDSVPALLVGRKAVIRLGLDAEICQSLLV
                     ESGEELAALRDALGHN"
     gene            complement(1603..>3805)
                     /locus_tag="VNZ06_01515"
     CDS             complement(1603..>3805)
                     /locus_tag="VNZ06_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWW19457.1"
                     /translation="AMVPLLFLTVLALSDKQKNINVSARDLAGLHRYQNLEAMLLPVG
                     MHEIWSAASVAGEGAVEKLQAATADVGKAVAAQDSVTEDYGAPAGEDARRWSEIKVAW
                     NAITAGKPSSVAEVVRLHSELRREILDYRDYIATTSGLVLDGDPVTFFMIDATVRQIP
                     NYESYVTEMRSHAAAVGAAGKATLADIEQITRSEVLAQGALDDIAADIRHAAEGGQAG
                     EVMRSRAEESIGQVKSAFEAFRKYVKQNVISGSISDPLDDVIFNAAQLTSAISSFHSS
                     MQATSQARLSDTLSKLRGSRTRMILLVGLALTVGIGFMGTVAWTTVGGMLAAVTVVSR
                     LAEGNYAREIYVYGNDEVARIMLALKQMQSKLSGVLSGVKESAITVATAARQIKAGTS
                     DLSSRTEQQAANLEETASSIEEMTTTVKQNADNAKLANKLAQAARDQAEHGGQVVEQA
                     VSAMGAIDIASKRISDIISVIDEIAFQTNLLALNAAVEAARAGDQGRGFAVVASEVRS
                     LAQRSASAAKEIKDLIHDSVGKVGEGSRLVSESGRHLHEIVASVKKVSDVVGEISNAS
                     QEQAASADEISRAVMQMDESTQQNAAMVEEANAAAASMNDQATRLSELTSFFKLREGN
                     ALPARTAEADAYKVSVKPGRPAPQIPAAARPSAVFASAAAAAAHSTAPAADKPAAAGG
                     GERPERRSSARPWVGVTPLVPPPAPAPAASTSASAKLRTASTASSNDWSEF"
BASE COUNT          628 a         1326 c         1183 g          668 t
ORIGIN      
        1 cgcgcgtgat ctgcgcacga ttgagcgtca ggcgatattc cgggtgcagc gtctcgggca
       61 ttcgcagcag cacccacagg aagacgatca gggcgtaggc gccgaacagc agaaagatcg
      121 agcgccacgg cgcaaccgcc agcaccagct gccccagcgt aggcgcgatc accgggatca
      181 tcaggaacag gatcatcgtc agcgacatga cgcgcgccag ctgccggcct gagaactgat
      241 cgcggaccgc ggagcgcgcc acgaccacgc tggcggcagc catgccgtgc accagccgca
      301 acagcagcag cgcgacgaaa ctggtggcca gtcccgacag caccgccgcg atcacgtaca
      361 ccgtcaggcc ggtgagcaga atcgggcggc ggccgtagcg atcggacagc agtccccaga
      421 acagctggcc gatccccatt ccggtcacat aggccgtgac gatcagctgg gtatggttcg
      481 gattcgcgac atgcagtgtg gcgccgatct ggggcagggc cggcagcatc gcatcgatgg
      541 cgatggcctg ggtggccatc agcatcccca ccagtgcgac gaactctttg aaacctactt
      601 tggaatcagg gagcgcggcg ctacccatga gcgcactcct gtggatgcac gtcagcgggg
      661 gatcagcgct cgatcctacc cgaaacggca ctcgggttgt gacgctcgct gcggatgact
      721 ctagttgtga ccgagggcgt cgcgcagcgc cgccagctcc tcacccgact cgaccagcag
      781 cgactggcag atctcggcat cgagcccgag gcgtatcacg gccttgcgac ccaccagcag
      841 tgccggcacg ctgtccggat cgtccttgca ggctgagagc atatcggcga cgtccagcac
      901 gtcggccagc gcgcccgcga cgccgcgcag ctcgcgcgac tgatcctcga agctatgcac
      961 cgcgatgacg atctcctcgg ccatgtacca gccctccagc agagccttgg cgatattgcc
     1021 gtgccagtcg tgcacgatgc gctgatacat ttcctgctcg ccgaacagcg ccggatgctt
     1081 cgtcgagtgc gtcaggatgt agagcttgcc gacaccgtgt accaggccgg tgagcatcgc
     1141 ggtatcgggg ctcgccttgt ccgtgcgtcg ggccaccacg aaggccagcg ctgcgaccag
     1201 cacgctctgc tgccacagcg cgctcagatg ccgctcgatg cccttgaact gtttggcacg
     1261 ctgaatctgt gccatcgcga agccgatcgc cgccgagcgc agcgaatcga agcccaagcg
     1321 cgtcaccgcc gtgcgcagat ccgagaccga cttgccgccc ggattgagtg ccgcggcgtt
     1381 ggccatcgac agcacgcggg tcgcgagcat cggctcggcg cccagcacgc ggaccacacg
     1441 cgcagaatcc gaatcatcct gcgacaggac cttctgcacc tgtgccgcga cctcgggaaa
     1501 gctcggcagc tcgatgtcac cggtcgtcag ctcggttgcc agctcgcgca cgaattcaaa
     1561 cgcgtcggtg ctggcctcac gctgcacagg cgcgctcatt cgttagaact cactccagtc
     1621 gttgctcgag gcggtgctcg ccgtgcgcag cttcgcgctc gccgatgtcg acgccgccgg
     1681 cgccggtgcc ggtgggggta caagcggtgt gacccccacc cacggacgcg ccgaactgcg
     1741 gcgttccggc cgctcgccgc caccagccgc cgccggcttg tcggccgctg gcgcagtgct
     1801 gtgcgcggcc gcggccgccg cactggcaaa taccgcactg ggccgtgccg ctgccggaat
     1861 ctgcggggca ggcctgcccg gcttgacgct caccttgtag gcatcggcct ccgcggtgcg
     1921 cgccggcagc gcattgcctt cgcgcagctt gaagaacgac gtcagctcgg acagccgtgt
     1981 ggcctgatca ttcatcgagg ccgccgccgc gttggcctct tcgaccattg ccgcattctg
     2041 ctgtgtgctc tcatccatct gcatcacggc gcgactgatc tcatccgcgc tcgccgcctg
     2101 ctcctgactg gcgttggaaa tctcgccgac cacgtcgctg actttcttga ccgatgcgac
     2161 gatctcatgc aaatggcggc cggactcgct caccaggcgg ctgccctctc cgaccttgcc
     2221 cacgctgtcg tggatgagat ccttgatctc cttggccgcc gaggcgctgc gctgtgcgag
     2281 gctgcgcacc tcgctggcga ccacggcgaa gccgcggccc tgatcgccgg cgcgcgctgc
     2341 ttcgaccgct gcgttcagcg ccagcaggtt ggtctggaat gcgatctcat cgatcaccga
     2401 aatgatgtcg gatatgcgtt tgctggcgat gtcgatcgca cccatcgcgg agaccgcctg
     2461 ttcgaccacc tgaccgccgt gctcggcctg gtcacgcgcg gcctgcgcca gcttgttggc
     2521 gagcttggcg ttgtcggcgt tctgcttgac ggtcgtggtc atttcctcga tcgaggaggc
     2581 ggtctcctcc aggttcgccg cctgctgctc ggtgcgcgag gacaagtcgg aggttcctgc
     2641 cttgatctgc cgggccgcgg ttgccacggt gatggcactt tccttcacac cgctcaatac
     2701 cccgctgagc ttgctctgca tctgcttcag cgcaagcatg atgcgggcaa cctcatcgtt
     2761 gccgtagacg tagatctcgc gcgcgtagtt gccttccgcc aggcgcgaca ccaccgtgac
     2821 cgcagcgagc atgcccccga ccgtggtcca ggccaccgtg cccatgaagc cgatgccgac
     2881 cgtcagcgcc agccctacca gcaggatcat acgggtgcgc gaacccctga gcttggagag
     2941 cgtatcgctc aggcgtgcct gtgaagtcgc ctgcatcgag ctgtgaaagc tgctgatcgc
     3001 ggaggtgagc tgcgcggcat tgaagatcac atcatccagc ggatcgctga tcgagccgct
     3061 gatgacattc tgcttgacgt atttgcggaa cgcctcgaag gcactcttga cctgcccgat
     3121 cgattcctcg gcgcgcgagc gcatcacttc accggcctgg ccgccttcgg ccgcatggcg
     3181 aatgtcggcc gcgatgtcat ccagggcacc ctgcgcgagc acttccgaac gcgtgatctg
     3241 ctcgatatcc gcgagcgtgg ccttgccggc ggccccgacc gcggcggcgt gcgagcgcat
     3301 ctcggtgaca tagctttcgt agttcggaat ctgacgcacc gtggcatcaa tcatgaagaa
     3361 cgtcaccgga tcaccatcca gcaccaggcc cgaggtggtc gcgatgtagt cgcgatagtc
     3421 caggatctcg cggcgcagtt ccgaatgcag cctgaccacc tcggccaccg aggacggctt
     3481 gcccgccgtg atcgcgttcc aggcgacctt gatctcgctc cagcggcgtg cgtcctcgcc
     3541 tgccggcgcg ccgtagtcct cggtgacgga atcctgcgcc gccacggcct tgccgacgtc
     3601 ggcggtcgcg gcctgcagct tctcgacggc gccctcgccc gccacgctcg ccgcgctcca
     3661 gatctcgtgc atgccgaccg gcagcagcat cgcttccaga ttctgatagc ggtgcagacc
     3721 tgccaaatcc cgcgccgaaa cgttgatgtt cttctgcttg tcgctcagtg ccagcaccgt
     3781 gagaaacagc aacggcacca tcgcc
//
LOCUS       DATMRM010000054         2854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1268122, whole genome shotgun sequence.
ACCESSION   DATMRM010000054 DATMRM010000000
VERSION     DATMRM010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268122"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VNZ06_01520"
     CDS             complement(<1..571)
                     /locus_tag="VNZ06_01520"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWW19458.1"
                     /translation="MKATRRWMWLGRSRHNAGLALLTVVLFVAVAARYQQFASAAGLR
                     DSFDDTSILILLALGQMLVMLTRAIDLSVAASVALTGMCVALINQAMPGVALPWLVLI
                     AALLGAALGAVNGLLVWQLQIPPIVVTLGTLAIYRGATYLISHGRWVNSNDMSPLFLS
                     LVRERWGGVTLLSWFALAGVLLTACLLRYS"
     gene            complement(568..2073)
                     /locus_tag="VNZ06_01525"
     CDS             complement(568..2073)
                     /locus_tag="VNZ06_01525"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HWW19459.1"
                     /translation="MTSNEASAKAPLVQLSSISKSFGGTRALESVDIALYAGSITALV
                     GENGAGKSTLVKILTGIHQPDGGRITVCGKSVRIRSPAHAQRLGISVIHQEAVVFDEL
                     SVAENIFVTARPRRWGLINWACMRRDAARLMERLDCPLDPGLRLRELSVAQKYLVQIA
                     RALSHDARLVIMDEPTAALSHHEAQLLLRIAAGLRDEGRALLFISHRLEDLLAIADRY
                     VVFRDGRRVAEGAMPGTSIDTLIQCMVGRVIERLYPKRRVALGEEVLRVCDLSRAGEF
                     ADVSFGVRRGEILGVYGLVGAGRSELMQSVFGLTRAESGRIELDRTQIRPRHPADAIA
                     QRIAYVPEDRQHQGAIPSLSISDNITLASLAQFARHGWVDGERLTRTAGEWAQRLQVK
                     YASLQQPLSELSGGNQQKVVLAKWLLTAPRVLILDEPTKGIDVGSKAAVHALMGEWVQ
                     QGMAIILVSSELPEVLGLSDRVLVMRRGRMAGMLDRSEADAAAVLQMASAA"
     gene            complement(2070..>2854)
                     /gene="rhaS"
                     /locus_tag="VNZ06_01530"
     CDS             complement(2070..>2854)
                     /gene="rhaS"
                     /locus_tag="VNZ06_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0033296 - rhamnose binding [Evidence IEA];
                     GO_process: GO:0015762 - rhamnose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HWW19460.1"
                     /translation="EGQIDVLNALIAQHVDAIAVSANDPDALVPTLKKALNRGIKVIS
                     FDSAVASGGRLVHLAPSSDVLIGQTCVQLAAAAAPSGHAQIAILSATPTSTNQNAWID
                     AMKAALPQYPQLALVATVYGDDLADKSYREAVALLKRYPDLKVIIAPTSVGIVAAAKA
                     LEDEGVAGKVLVTGLGLPSELAGHVTAGSVKSFAIWNPIDLGYAAVQLAVHIVRGTPA
                     GPGATIAIGRLGVVHFDSNGIGPMGKPFVYDQSNVMDFAKVF"
BASE COUNT          545 a          977 c          868 g          464 t
ORIGIN      
        1 cgctatagcg caataaacac gccgtgagca gcacaccggc gagcgcgaac caggacagca
       61 aggttacgcc gccccagcgc tcgcgcacca gactcaggaa caagggcgac atgtcgttgc
      121 tgttgaccca acggccgtgc gagatcagat aagtcgcgcc gcgatagatc gcgagcgtcc
      181 ccaacgtaac cacgatcggc ggaatctgca actgccagac cagcagcccg ttgacggcac
      241 cgagggccgc gccgagcagc gccgcgatca gcacgagcca cggcagcgcc accccaggca
      301 ttgcctgatt gattagcgcc acacacatgc cggtcagtgc cacactggcc gcgaccgaca
      361 gatcaatcgc gcgcgtcagc atcaccagca tctggccgag cgccagcagg atcagaatcg
      421 atgtatcgtc gaagctgtcg cgcagccccg ccgcgctcgc aaactgctgg tagcgcgccg
      481 cgacggccac gaacagtacc acggtcagaa gcgcgagccc cgcgttgtgc cgcgagcggc
      541 caagccacat ccaacggcgc gtcgctttca tgccgcgctc gccatttgca gtaccgcggc
      601 cgcatcggcc tcgctgcgat caagcatgcc ggccatgcgt cctcgccgca tcaccagcac
      661 tcggtccgac agaccgagca cctcgggcaa ttccgacgac accaagatga tcgccatgcc
      721 ctgctgcacc cattcgccca tcagggcgtg cacggcagcc tttgagccca cgtcgatgcc
      781 cttggttggt tcatccagga tcagcacccg tggcgcggtc agcaaccatt tcgccagcac
      841 caccttctgt tgattgccgc ccgagagctc actcaagggc tgctgaagac tcgcgtactt
      901 cacctgcagt cgctgcgccc actcaccggc tgtgcgtgtc aggcgctcgc catcgaccca
      961 cccatggcgg gcaaactggg cgagcgaggc cagcgtgatg ttgtcgctga tcgacagtga
     1021 cgggatggcg ccctggtgct gccggtcctc gggtacatag gcgatgcgct gtgcgatcgc
     1081 gtcggccgga tgccggggcc ggatctgggt gcgatcgagc tcgatgcggc ccgattcggc
     1141 gcgcgtcagc ccaaacacgc tctgcatcag ttcgctacgt ccggcgccaa caagcccata
     1201 gacacccagg atctcgccgc ggcgcacgcc gaaactgaca tcggcaaatt ccccggcgcg
     1261 tgacagatcg cacacgcgca atacctcctc gccgagcgcg acgcgtcgct ttgggtagag
     1321 ccgctcgatc acgcggccga ccatgcactg aatcagtgtg tcgatgctcg tgcctggcat
     1381 cgccccctcg gctacacgtc tgccatcgcg aaacacgacg tagcgatccg cgatcgccaa
     1441 cagatcctcc aacctgtggc tgatgaatag caacgctcgc ccctcatcgc gcagcccggc
     1501 cgcgattcgc agtagcagct gcgcctcatg atgcgacagc gctgcggtgg gctcatccat
     1561 gatcacgagg cgcgcatcgt ggctgagcgc gcgggcgatc tgcaccagat acttctgcgc
     1621 cacggacagc tcgcgcaatc tcaagcccgg gtcaagcggg cagtccaatc gctccatcag
     1681 ccgcgcggca tcgcgccgca tgcacgccca gttgatcaga ccccagcgcc gcgggcgcgc
     1741 agtcacgaag atgttctcgg caaccgacag ctcatcgaac accaccgctt cctggtgaat
     1801 cacactgata ccaagccgct gcgcgtgcgc tggggagcgg atgcgcacgc tcttgccgca
     1861 gaccgtgatg cgcccgccat cgggttggtg gatgccggtg aggattttga ccagcgtgga
     1921 tttgccggcg ccgttttcac cgaccaaggc ggtgatcgaa cccgcgtaaa gcgcgatatc
     1981 gaccgactcg agcgcgcgcg taccgccgaa ggacttgctg atgctggaca gttgcaccag
     2041 gggggccttc gccgatgcct cgttgctggt caaaagacct tcgcaaagtc catgacattg
     2101 gattggtcat agacaaacgg cttgcccatc gggccaatgc cgttgctgtc gaagtgcact
     2161 accccgaggc ggccgatggc gatcgtggcg cctggccccg cgggcgtgcc gcgcacgatg
     2221 tggacggcga gctgcacagc agcgtagccg agatcgatcg gattccagat cgcaaagctc
     2281 ttgacagagc cggccgtcac gtggcccgcc agctcggaag gcaggccgag ccccgtgacc
     2341 agcaccttgc ctgccacgcc ctcgtcctct agcgccttcg ccgccgcgac gatcccgacc
     2401 gaggtcggcg cgatgatgac tttgagatcc ggataacgct taagcagtgc gaccgcctcg
     2461 cgataggact tatcggccaa atcatcgcca tagacggtgg cgacgagtgc aagctgcggg
     2521 tattgcggca gcgccgcctt catcgcgtcg atccaggcgt tctgattggt cgaggtcggt
     2581 gttgccgaca gaatcgcgat ctgggcgtgg cccgagggcg ctgcagcagc ggccagctgc
     2641 acgcaggtct gcccgatcag cacatccgat gaaggcgcga gatgcaccag gcgaccgccg
     2701 cttgcgaccg ccgaatcgaa ggaaatcacc ttgatgccgc ggttcagtgc cttcttgagc
     2761 gttggcacca gcgcgtccgg atcattggca gaaacggcga tcgcatccac gtgctgtgca
     2821 atgagtgcgt tgagcacgtc gatctggcct tcgg
//
LOCUS       DATMRM010000055         7246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3433309, whole genome shotgun sequence.
ACCESSION   DATMRM010000055 DATMRM010000000
VERSION     DATMRM010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3433309"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VNZ06_01535"
     CDS             complement(<1..469)
                     /locus_tag="VNZ06_01535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWW19461.1"
                     /translation="MSAFATLASRTDDLVPHGKVHLRETTHDGARALDAFLLGIERRA
                     FRIAQIALSHEADALDVVQDAMLQLSTRYASRPAEEWKPLFYRILQNRIRDVQRRRSV
                     RNRVMALMPWRSDDDEEEIDPIAQAVDHAPLPPSQLEGDEMMQALERALNELPP"
     gene            745..3015
                     /locus_tag="VNZ06_01540"
     CDS             745..3015
                     /locus_tag="VNZ06_01540"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HWW19462.1"
                     /translation="MLSTELSTGIDDPGTTVEGVLRVAVNAPMRSPFEYLAPPGVAVA
                     DVLPGVRVRIPLGRRSTIGVVVDHSPRPSIGAVALKAVQELLDPSPLLDGALLQLLRW
                     TAQYYHHPLGEVIAAALPKSLKLGAPAVGQVTLWRLTGPGHDALAAGSAARARSQLSL
                     MTLLARYPGGLPAADLAAQLPKWRSAALALRKRGWIDSREVAASATDLPLEGLPRDVL
                     PKSPELSAEQSHAVAQIDAGSGRYETFVVQGATGSGKTEVYLQCASRVLARGGSVLVL
                     VPEIGLTPQLVQRFSERLSTRIAVMHSALGDAERLAAWRAARDGSARIVLGTRSAVFA
                     PLPGLQLIVVDEEHDSSYKQYDGGCRYSARDLAVVRAHQSGIPIVLGSATPAFETLHN
                     STRGRYRTLLLPRRADQAPPPHLALVDLRAHAMRQGLATPVTDAIRRHLAQRGQVLVF
                     INRRGYAPTLLCTSCGWIAPCPDCDARLTVHNTQQQLRCHHCGHAETLPERCTRCGFT
                     VKPVGQGTERVEQTLRELFPAEGLMRLDRDSASSARELEAIMQSVHSGQTRILVGTQM
                     ITKGHHFPDVSLVVVLNADQGLFSTDYRAAERLAQTIVQVAGRAGREKLRGEVLLQTE
                     YPDHPLLQSLLSGGYEAFAATALAEREAARWPPFGRLALLRASSDTSQGALEFLSAAR
                     ALAPDSPQALVRVLGPVPATMARRAGRYYAQLLIESAERAPLHHFIEEWLPQIERLGR
                     SRRVRYVLDIDPIDIQ"
     gene            3077..4834
                     /gene="argS"
                     /locus_tag="VNZ06_01545"
     CDS             3077..4834
                     /gene="argS"
                     /locus_tag="VNZ06_01545"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HWW19463.1"
                     /translation="MKRELERLIAEALKRLSGTLLPEGVDPAAVNVDRSRDAGHGDFA
                     SNVALRLAKQARQSPRQLAEAIVAAMPASALLARAEVAGAGFINFYLAPSAQNAALRQ
                     VFEQADRFGQSELAGGESLIIEFVSANPTGPLHVGHGRQAAYGATLANLLSAIGHRVH
                     REFYINDGGRQMDILTVSTWLRYLEACGESVPFPSNGYRGDYVRDLASELYQRCGREL
                     QRSAAEVMAALPPDAPAGDQEQHIDALIARMTLLIGDTRRGDVYDMALSLMLAGMRQD
                     LADFGVEFDSWASERALARNGAVEHAIETLQAKGALYSKDGAVWFRATQYGDDEDRVL
                     IRSNGVKTYFAPDIAYHLQKCERGFKRLVDVLGADHHGYVARVRGALWAMGQDGDALE
                     ACLIQFVSLFRGGEKIAMGKREGQFVTLRQLRAEVGSDACRLFYLMRSHDQHLDFDLE
                     LAKSRTNENPVYYIQYAHARVASVLRQLAARELQYDPSMAFENLQRLDSAPEQATLRE
                     LARFPEIILQAARARAPHTLVHYLRDLATAFHTWYNAATFIVEEAPLRNARLALALGV
                     RQVIRNGLTLLGVSAPDSM"
     gene            4831..5436
                     /locus_tag="VNZ06_01550"
     CDS             4831..5436
                     /locus_tag="VNZ06_01550"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HWW19464.1"
                     /translation="MSGRRLTGRDYKRTRHSGLALNLSRLRDHAIGFGAGLCVAGLIY
                     VATHRAGEPPAQSPRPTPRNTARMEGAGGDPNPADASLNTLPPNAEGKYDFYQMLPKF
                     EVVVPEKERGTRVAPTAQIDRPGIYFLQVGSYRDADVAERVHTQLTRLGIDSTVQRVA
                     VDSDVWHRVRVGPIKDLPRLNRIREQLRASDLDSLVIRVDE"
     gene            5429..6379
                     /locus_tag="VNZ06_01555"
     CDS             5429..6379
                     /locus_tag="VNZ06_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19465.1"
                     /translation="MSDASVLAGRLCGRATGLCLYGFAPPRLATAEHELREIAAHQAE
                     RIRLLGADGVIVYDIQDESDRTAVPRPFPFLPTVDPTDYALEQLKTLEVAKIVYRSVS
                     RDSTESFARWLTRISHRQQAMLAVLVGAPSRRSQPALTLSEAYALRAQHAPSLIVGGV
                     AISERHARSVNEHQRMLDKTQNGCRFFITQAVYDVTSAKSLLSDYAIAAQRLGQPPLP
                     VIFTFSPCGSVKTLAFMQWLGISFPRWLENELRFSSDPLGKSVTLCERIFAELWEYAN
                     DKALPIGVNVESVSVRKVEIEASLELFRRLRSRIGASAPL"
     gene            6534..6857
                     /locus_tag="VNZ06_01560"
     CDS             6534..6857
                     /locus_tag="VNZ06_01560"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWW19466.1"
                     /translation="MNDEQKFSSIRKGLLEFLVLTIISARTVYVADILKRLAATDFAT
                     QEGTLYPLLSKLRREGLVNYEWQESESGPPRKYYGLTPTGKKHLAELDEYWAGITRTV
                     TELRS"
     gene            6862..>7246
                     /locus_tag="VNZ06_01565"
     CDS             6862..>7246
                     /locus_tag="VNZ06_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19467.1"
                     /translation="MRKVITISLNGNAFQLEDDAYAALAAYLEEAARALAANPDRAEI
                     ISDLEQAIADKCATCLSPHKSVVTRDEIQRVIAEMGTVDADPAPRAGGSANAGAGQTH
                     NPGADFGAHGAGEPKGAPRRLYQISE"
BASE COUNT         1322 a         2296 c         2334 g         1294 t
ORIGIN      
        1 gcggtggcaa ctcgttcagt gcccgctcca acgcctgcat catttcgtcg ccttccagct
       61 ggctgggggg caacggcgca tggtcgaccg cctgagcgat cggatcgatc tcttcctcgt
      121 cgtcatcgga gcgccagggc atcagcgcca tgacgcggtt gcgtacgcta cggcgacgct
      181 gaacgtcgcg gatgcgattt tgcagaatcc ggtagaaaag cggcttccat tcctccgcgg
      241 gacggctagc gtaccgagtg ctcagttgca gcatggcgtc ctgcacgacg tcgagtgcat
      301 cggcctcgtg gctcaacgct atttgtgcaa tcctgaaggc cctgcgctcg atacccagca
      361 gaaacgcgtc gagcgcccgt gcgccatcat gggtcgtttc acgcaaatgg accttcccat
      421 gtgggacgag atcatccgtt cgggaagcaa gggtagcaaa cgcggacata ttgcaaaaat
      481 ctatcctggc tgaatcctct ctacgcgtgc tcaacggagc agaaacatct ttggttgaca
      541 accgttaata aatgcattgg ttcggaaaat catccactta actgctcgga cgcgctgctt
      601 ggccaatgct gtgagacagg tccggtgcgc cgggccgcgg ggggcgcaca tgtggctgat
      661 aatgatgcga atttcgagtt aatcgacttt taaccaatct gtcatgaact tattgaatcg
      721 ccagcaattg tagtgtcggg ccggatgcta tccacagagt tatccacagg aatcgatgac
      781 cccgggacga cggtggaagg cgtccttcgg gtcgctgtca atgccccgat gcgcagcccg
      841 ttcgagtacc tggcgcctcc cggtgtggcg gtagccgatg tgctcccggg agtccgggtg
      901 cgcataccct tagggcgccg cagcacgatc ggtgtggtgg tggatcactc tcccaggccc
      961 tcgatcggtg cagtcgccct caaggcagtg caggagttgc tcgatcccag tcccctgctc
     1021 gacggcgctc tgctgcagct gcttcgctgg acggcacagt actaccatca cccgttaggc
     1081 gaggtcattg ctgccgccct gccgaaatcc ctcaagctcg gagccccggc ggtcggccag
     1141 gtcacgctct ggcgtttgac cggccccggg catgatgccc tggcggccgg cagcgccgcg
     1201 cgagcgcgca gtcagctgag cctgatgacc ctgctcgccc gctaccccgg cggcctgccc
     1261 gccgctgacc tggcagcgca actgccgaag tggcgaagcg ccgcgcttgc tttgcgcaaa
     1321 cgtggctgga tcgattcgcg cgaggtcgca gccagtgcga cagacctccc cttggagggg
     1381 ctaccccgtg acgtgttgcc caagagcccg gagctctcgg ccgagcagag ccacgccgtg
     1441 gcacagatcg acgcgggtag cggccgctac gagacgtttg tcgtgcaggg agccacgggc
     1501 agcggcaaga ccgaggtcta cctgcagtgc gcgtcgcgtg tgctcgcgcg cgggggctcg
     1561 gtgctggtgc tggtaccgga aatcggattg acaccgcagc tcgtgcagcg atttagcgag
     1621 cggctcagta cccggatcgc cgtcatgcat tcggccttgg gcgacgctga gcgtctggct
     1681 gcctggcgcg ctgcccgtga cggcagtgca cgcatcgtat taggaacccg atcggccgtg
     1741 ttcgccccgc ttcccggcct gcagctgatt gtggtcgacg aagaacacga cagctcgtac
     1801 aagcagtacg acggcggctg ccgctattca gcgcgcgatc ttgcggtggt gcgcgcgcac
     1861 cagagcggta ttcccatcgt gctcggctct gccacaccgg cattcgagac cctgcacaac
     1921 tcaacacgcg gacgctaccg cacgctgttg ctgccgcgac gggccgacca ggcaccacca
     1981 ccgcatcttg ctctggtgga cctgcgcgca cacgcgatgc gccaggggtt ggccactccg
     2041 gtaactgacg ccattcgacg gcatctggcg cagcgcggac aggtactggt gttcatcaat
     2101 cgccgcggct acgcccccac cctgctgtgc acctcctgcg gctggatcgc gccctgtccg
     2161 gactgcgatg cgcgtctgac cgttcacaac acgcaacaac aactgcgctg tcatcactgc
     2221 ggacacgctg aaaccctgcc cgagcgctgc acgcgttgcg gcttcactgt caagccggtg
     2281 ggtcagggaa ccgagcgcgt cgagcagacg ctgcgtgagc tgttcccggc ggaaggcctg
     2341 atgcgcctcg accgcgacag tgccagcagc gcgcgcgagc tggaagcgat catgcaaagc
     2401 gttcatagcg ggcagacgcg catcctggtc ggcacgcaga tgatcacgaa aggccatcac
     2461 tttccggacg tctcgctggt ggtcgtattg aatgccgatc agggcctgtt cagcactgac
     2521 taccgggcgg ccgaacgact tgcgcagacg atcgtgcagg tcgcaggccg cgcggggcgc
     2581 gagaaactgc gtggtgaagt actgctgcag accgaatatc ccgatcatcc attgctgcag
     2641 agcctgctca gcggaggcta tgaagcattc gccgcgacgg cgctggccga gcgcgaggct
     2701 gcgcgctggc cgccgttcgg cagacttgcg ttgttgcgcg cctcgagcga caccagccag
     2761 ggcgcgctcg agttcctgag tgcggcgcgt gccctggcgc cggactcacc gcaagcgctg
     2821 gtacgggtgc tgggaccggt accggcaacc atggcgcgac gcgccggacg ctactatgcg
     2881 caactgctga tcgagtcggc cgagcgagcg ccgttgcatc acttcatcga ggagtggttg
     2941 ccgcagatcg agcgtctggg ccggagccgg cgcgtgcgct acgtcctcga catcgatccg
     3001 atcgacattc aatgaccgcg ggttgccgct gcaaggtaga ctcgcggccc gcgggcaacg
     3061 ggaaggaagc aaggaattga agcgtgaact ggagcgcctg atcgccgaag cgctgaagcg
     3121 cctgagcggc acgctgttac cggaaggggt cgatccagcc gccgtcaatg tcgaccgatc
     3181 acgcgatgcc ggacatggcg acttcgccag caatgtcgcg cttcggctgg cgaagcaggc
     3241 gcgccaatca ccgcgacaac tggccgaagc catcgttgca gcgatgcccg ccagtgcttt
     3301 gctggcgcgc gccgaagttg ccggagccgg cttcatcaac ttctaccttg cgccgagcgc
     3361 acagaacgcg gcgctgaggc aggtgttcga gcaggccgat cgcttcgggc aaagcgagct
     3421 cgccggcggc gaatccctga tcatcgaatt cgtctccgcc aatccgaccg gaccattgca
     3481 cgtcggccac ggtcgccagg ccgcttatgg cgccacgctc gccaatctgc tcagtgcgat
     3541 cgggcatcgc gtgcaccgtg agttctacat caacgacggc ggccggcaga tggacatcct
     3601 gacggtgagc acctggctgc gctatctgga agcttgcggc gagagcgtgc cgtttccctc
     3661 caacggttat cgcggcgact atgtgcgcga ccttgccagc gagctctacc aacgatgcgg
     3721 ccgtgagctg caacgctccg cggccgaggt gatggccgct ctgccgccgg acgctccggc
     3781 aggcgaccag gaacagcaca tcgatgcgct gatcgcacgc atgaccctcc tgatcggaga
     3841 tacacggcgc ggcgacgtgt atgacatggc gctgtcactg atgctggcgg gcatgcgcca
     3901 ggatcttgcg gacttcgggg ttgaattcga tagctgggct tccgaacgcg ccctggcgcg
     3961 caacggtgcg gtcgagcatg cgatcgagac gctgcaggca aaaggggcgc tgtattcgaa
     4021 ggatggcgcc gtgtggttcc gggcgacgca gtacggcgat gatgaagacc gcgtgctgat
     4081 tcgatccaat ggggtcaaga cctattttgc ccctgacatc gcgtatcacc tgcagaaatg
     4141 cgagcgcgga ttcaagcgcc tggtcgatgt gctcggagca gaccatcacg gctacgtggc
     4201 acgggtgcgc ggtgcactgt gggcaatggg gcaggacggc gacgcgctcg aagcctgcct
     4261 gattcagttc gtcagcctgt ttcgcggtgg cgagaagatc gcgatgggca agcgcgaagg
     4321 gcagttcgtc accttgcgcc agctgcgcgc ggaagtcggc agcgacgcct gtcggctgtt
     4381 ctatctgatg cgcagccatg atcagcacct ggatttcgac ctggagctgg ccaagtcgcg
     4441 caccaacgag aatccggtct actacattca gtatgcgcac gcgcgcgttg caagcgtgct
     4501 gcggcagctc gctgcgcgtg agctgcagta cgatccgtcc atggcgttcg agaaccttca
     4561 gcgcctggac agcgcgcctg aacaggcgac gctgcgcgaa ctggcgcgct ttccggaaat
     4621 catcctgcag gcggcgcgcg cacgcgcgcc gcacacattg gtgcactatc tgcgagatct
     4681 cgcgaccgcg tttcacacct ggtacaacgc ggcgactttc atcgtcgagg aagcgccgct
     4741 gcgcaatgcg cgactggcac tggcgctcgg ggtgcgccag gtgattcgca acggactgac
     4801 gctgctcggc gtatctgcgc cggatagcat gtgagcggcc gacgtcttac cgggcgcgac
     4861 tacaagcgca cgcgacactc cggcttggcg ctgaatctgt ctcgcttgcg cgaccacgcg
     4921 attggatttg gcgcgggtct gtgcgtggcc ggcttgatct atgttgcgac ccatcgggcc
     4981 ggcgagccgc ccgcacagag cccccggccc actccacgca acaccgcccg tatggagggt
     5041 gccggcggcg atcccaaccc ggcggatgcc tcgctgaaca cgctgccgcc gaacgcggaa
     5101 gggaaatacg atttttacca gatgctgccg aagttcgagg tggtcgtgcc ggagaaggaa
     5161 cgcggcacgc gcgttgcgcc gacggcgcaa atcgatcggc ccggtatcta cttcctgcaa
     5221 gtcggatcct atcgcgacgc ggacgtcgcc gagcgcgtcc acacacagct cacccgactt
     5281 ggcatcgact ccacggtgca gcgcgtggcc gtagacagcg atgtgtggca tcgagtgcgc
     5341 gttggcccca tcaaggacct gccccggctc aatcgcattc gcgagcagct gcgggcaagc
     5401 gatctggatt cgctggtcat tcgcgtcgat gagtgatgcc tcggtgcttg ccggcaggct
     5461 ctgcggccgc gcaaccggcc tgtgtctata cggctttgcg ccgcccagac tggcgacggc
     5521 cgagcacgag ctgcgcgaga tcgccgctca ccaggcggag cgaatccggc tgcttggtgc
     5581 ggacggtgtc atcgtgtacg acattcagga tgaatcggac cgcacagccg tgccccgacc
     5641 ttttccattt ctgccgaccg ttgatccgac ggactacgcg ctcgagcagc tgaagacact
     5701 ggaggtcgcc aagatcgtct atcgcagtgt cagtcgcgat agcaccgaat cgttcgcgcg
     5761 ctggcttacc cgtatcagcc accggcagca agcgatgctc gccgtactcg tgggagcacc
     5821 cagtcgccgc tcgcaacccg cactgacgct ttcagaggcc tatgcgctca gggcgcaaca
     5881 cgcgccgagt ctcatcgtgg gcggcgtcgc catctcagag cggcacgcgc gtagtgtgaa
     5941 tgagcatcag cgcatgctcg acaagactca aaacggctgc cgctttttca tcacgcaagc
     6001 ggtctatgac gtcacctcgg cgaagtcact gctgtccgat tacgccatcg ctgcgcagcg
     6061 cctcggacag ccgccgctac cggtcatctt taccttctcg ccgtgcggat cggtgaagac
     6121 gctggccttc atgcagtggc tgggcatttc gtttccgcgc tggctcgaga acgaattgcg
     6181 tttcagcagc gacccgctcg gcaaatccgt cacgctgtgt gaacgaatct tcgcggagct
     6241 gtgggagtac gcgaatgaca aggcgttgcc gatcggcgtg aacgtggaga gcgtgtcggt
     6301 ccgcaaagtc gagatcgaag cctcgctgga actgtttcgg cgattgcgga gccggatcgg
     6361 cgcctcagcg ccgctttgac gctctcaccc gcgatcgcgg cctggtactc ccgggcgggc
     6421 tgaaatgccc cactgatcaa tatgttgtgc gctctgcggc gcagcaggtg tcgttttgtt
     6481 gacgaccggg cctgagactg gtaccttgtc tgataaaggt agcttacaaa tacatgaacg
     6541 acgagcagaa attcagttcc atccgcaaag gcctgctgga gtttctggtg ctcacgatca
     6601 tcagtgcaag gactgtctac gtcgccgaca ttctcaagcg tctcgcggcg acggatttcg
     6661 cgacccagga ggggacgctc tatccgctgc tcagcaagct gcgccgggag ggactggtca
     6721 actacgagtg gcaggagtcg gagagcggac ccccgcgcaa gtactacgga ctgacaccaa
     6781 ccggcaagaa gcacctggcc gagctggatg aatactgggc tggcatcaca cggaccgtta
     6841 cagaattgag gagctaaggt tgtgcgcaaa gtcatcacga taagcctcaa cggcaacgcc
     6901 ttccagctgg aagacgacgc gtacgcggct ctggccgcct atctggaaga agctgcacgg
     6961 gcgctggccg ccaatcctga ccgggcggaa atcatctccg acctggaaca ggcgatagcc
     7021 gacaagtgcg cgacctgcct cagcccacac aagagcgtcg tgacacgcga cgagatccag
     7081 cgggtgattg cggaaatggg aacggtcgac gccgatccgg cgcccagggc cggtgggtcc
     7141 gcgaatgccg gcgccgggca gacgcacaac ccgggagcgg atttcggcgc gcacggcgcg
     7201 ggcgagccca agggggctcc gcgtcgtctc tatcagatca gcgaag
//
LOCUS       DATMRM010000056         4556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3120364, whole genome shotgun sequence.
ACCESSION   DATMRM010000056 DATMRM010000000
VERSION     DATMRM010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3120364"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..619)
                     /locus_tag="VNZ06_01570"
     CDS             complement(59..619)
                     /locus_tag="VNZ06_01570"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HWW19468.1"
                     /translation="MKNTFKKLPSRQRGMVMFIALILLLVLTLLGVALARMQTVEERL
                     AQNDANHAMALQTAEAALQAAFDNDAAGVYTDWSGATPGVYSVSAELGGGTSTAGYQT
                     TWTPGVNSIAYAGSGPALASAPAAATPAYVLEALAPAAPQGCSIGGGGTGYPPVVIFV
                     HRITAHSQGGDGSASATVQTVHIGGC"
     gene            complement(627..1763)
                     /locus_tag="VNZ06_01575"
     CDS             complement(627..1763)
                     /locus_tag="VNZ06_01575"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HWW19469.1"
                     /translation="MSARKHRGFSLVELMVAMVISVIIVDAAVSLFLANRQTGNTTAA
                     TSAIADNGRIAMSFISEAVRSGGYMACNATNDVRAIVTGQTRQISIISPGPTPVQRNY
                     LQAFGGYEAAGTLPNNAVAFAIAKPPVLADGTAGDWINAGGLDAALVGKVTKGSDVLV
                     VRESLPQSTPIYTTSYYATGSGIPTLNVDTVGTLTPGQYAVISDCSFSTAFQVGAVNA
                     GALTIGTAGAQDLFGGDLKLSYTNPASITPVNMYIYFIGPGRDTDSSLWVYDESQGPP
                     PAGFFELVPDVENMQVLYGIAPTNPNQATQYVTADAVVDFNQVVAVKVALLVASPLNA
                     SSVQTATALTVQNFSLLYTTVTPPLDARLRKVFDTTIAVRSAAL"
     gene            complement(1760..2281)
                     /gene="pilV"
                     /locus_tag="VNZ06_01580"
     CDS             complement(1760..2281)
                     /gene="pilV"
                     /locus_tag="VNZ06_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HWW19470.1"
                     /translation="MKRQQTLAAGFTLVELMVAMAVLAIGLLGIAKLSLGTVQANGSA
                     FMRTQATQLIQQIVDDMRANHDQATLNLGYNIALGANPGAAPNCITAACSAAGIASFD
                     LARWFTQLATLLPGGKGGVTVATVTAPLSGSTFEYQATITVTWDDTVATQAFANGSIP
                     ATNTQTVVMETML"
     gene            complement(2302..2904)
                     /locus_tag="VNZ06_01585"
     CDS             complement(2302..2904)
                     /locus_tag="VNZ06_01585"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HWW19471.1"
                     /translation="MAIRLNGTGAVTRTSGFSLLELLVTIGIAGILAAIAVPAMRTYV
                     QTYQASNAASSLVVALNYARSEAIKEDVPTAGGAGITICASTLATAPPTCDTGSWASG
                     WIVLTPNVALGNAGVLQQSGALPATFTLTQAPANATIVFQPNGTAPALVGDINGRSSF
                     KLCDARGAKFARELEVSRTGIIQASQTLGQDVSGAALTCP"
     gene            complement(2982..4343)
                     /locus_tag="VNZ06_01590"
     CDS             complement(2982..4343)
                     /locus_tag="VNZ06_01590"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HWW19472.1"
                     /translation="MTVTRRLLLLMLPALAIVMFGGGVIDYLIAVDATRNSYDQALDN
                     SALTLASLIRLEDGKLDVLTPEREGAQRSRFDRQSYAIVSPEGTLLAGNAALVSKARS
                     TAADPLYYDASAAGRALRVVRTRSQTPAGPVTIYLGETVQRRERAERAMLVGKLSVDF
                     VELDLALILVWISVYVGLRPLRRLHDVVEASAARELRAFSETEVVGEVRPLVRAFNQL
                     LELLQDAAASQRRFVADAAHQLRTPIAGLSAQIELLLHNPGAAALAPELSTINQGIAR
                     VARSANQLLSLARTEQSSLQSEKFEPVALRPMLEELIGRNVDRAESLGLDLGAELQDA
                     TLQGDIWMLQDLAGNLLDNALKYTPRGGHVTVRCGLGPQRAPYVEVEDDGPGIPESER
                     RRVRERFYRPSGSAAPGCGLGLAIVEEIARVHRATLSIEGGAGNTGARMRVRFTPQGG
                     NVT"
     gene            complement(4340..>4556)
                     /locus_tag="VNZ06_01595"
     CDS             complement(4340..>4556)
                     /locus_tag="VNZ06_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWW19473.1"
                     /translation="IVLESLAHSIGRVVTKERLQQDLASGEQELSNNAIETQVSRLRA
                     KLGDAAVIRTIRGLGYRLDEGAALKPS"
BASE COUNT          836 a         1478 c         1407 g          835 t
ORIGIN      
        1 gcaacgatta atgtgccgcg ggtcggcttg gaaatagaca tcttgctctc gaacatgatc
       61 agcaaccccc aatgtggacg gtttgcaccg tggcgctggc actgccgtcg ccaccttgcg
      121 aatgagcggt aatacggtga acaaaaatga cgacaggagg gtatccggtt cccccgccgc
      181 ctatcgaaca gccctgcgga gcggccgggg ccagtgcctc gagaacgtag gcaggagtcg
      241 cggctgccgg cgccgacgcc agcgccggac cactgccggc gtaggcaatc gagttcacgc
      301 ccggagtcca ggtcgtctgg tagcccgccg tgctggtgcc accgccaagt tccgccgaga
      361 cggagtaaac ccctggggtc gcaccgctcc agtcggtgta gacaccggcg gcgtcattgt
      421 cgaacgcggc ttgcagggct gcttcggctg tctgcaatgc catcgcatga ttggcgtcgt
      481 tctgcgccag tcgctcttca accgtctgca tgcgggccag tgccacgccg agcagcgtca
      541 gcaccagcag caggatcagc gcgatgaaca tcaccatgcc gcgctggcgg gagggaagtt
      601 ttttgaaggt gtttttcata taccctctag agtgccgcgc ttcgcacggc aatggtcgta
      661 tcgaaaacct tgcgcagtct ggcatccagc ggcggtgtca cggtcgtgta gagcagcgag
      721 aagttctgca ctgtcaaggc agtcgcggtc tgaacggaac tggcattcag aggactggct
      781 accagcagtg ccactttcac cgcgaccacc tgattgaagt ccacgaccgc gtcggccgtc
      841 acgtactgcg tcgcctgatt gggattcgtc ggagcgatgc cgtacaacac ctgcatattc
      901 tcgacatccg ggaccagttc gaaaaaacca gccggagggg gaccctgcga ttcgtcgtag
      961 acccacagcg aagagtcagt gtcacgaccc ggtccgatga agtagatgta catgttgacc
     1021 ggcgtgatcg aggccgggtt tgtataggac aacttcaaat ccccgccgaa tagatcctga
     1081 gcaccggccg taccgatggt cagcgcgccg gcattgacgg cgccaacctg gaaggcagtg
     1141 gagaacgagc aatcggagat caccgcgtac tgacccgggg tcaaggtgcc caccgtgtcg
     1201 acgttaagcg tcgggatgcc ggaaccggtt gcgtagtagc tcgtggtgta gatcggcgtg
     1261 gattgcggca gcgattcacg cacgaccagc acatcgcttc ccttggtgac tttgccgacc
     1321 agcgcggcat ccaggccgcc ggcattgatc cagtctcccg ccgtgccgtc agccagcacc
     1381 ggcggtttcg caatcgcgaa cgctactgcg ttgttaggca gtgtgcccgc agcctcgtag
     1441 cctccgaagg cctgcaggta gttgcgctgc acgggcgtcg ggccgggaga gatgatgctg
     1501 atctggcggg tctgtccggt gacaatcgcg cggacatcgt tggtcgcgtt gcacgccatg
     1561 tagccgccgc tgcgaaccgc ctcgctgatg aaactcatgg cgatgcggcc gttatccgca
     1621 atcgctgagg tcgccgcggt ggtgttgccg gtctgacgat tcgcgagaaa caacgacacc
     1681 gccgcatcca cgatgatcac cgagatcacc atcgcgacca tcagctccac cagcgagaag
     1741 ccacgatgtt ttctggcgct cacagcatcg tctccatcac aaccgtctgc gtgttggtcg
     1801 ccggtatcga tccattggca aatgcctgag tcgccaccgt atcgtcccag gtcacggtga
     1861 tcgtggcctg gtattcgaac gtgctaccag agaggggagc ggttaccgtc gcgaccgtaa
     1921 ccccaccttt tccgccgggt aacagggtgg ccagttgcgt gaaccagcgc gccaggtcga
     1981 agcttgcgat gcctgcggcg ctgcacgccg ccgtgatgca gttgggtgcg gcacccgggt
     2041 tggcaccgag cgcgatgtta tagcccagat tgagcgttgc ctgatcgtga ttggcgcgca
     2101 tgtcatcgac gatctgctgg atgagctgtg ttgcctgggt gcgcatgaac gcactgccgt
     2161 tggcctgcac agtgccgagg gataacttgg cgattccgag tagcccgatc gccagcaccg
     2221 ccatcgccac catcagttca accagtgtga atccagccgc gagtgtttgt tgccgcttca
     2281 tgagtttttc cccagcttca ttcatgggca ggtcagcgcc gcgccggaca cgtcctggcc
     2341 cagcgtctgc gaggcctgga tgatgccggt cctgctgact tccagctcgc gggcaaactt
     2401 cgcgccacgg gcgtcgcaca gcttgaacga gctgcgaccg ttgatatctc ccaccaatgc
     2461 cggtgcggtg ccattgggct gaaacacgat cgtcgcattg gcaggtgctt gcgtcagcgt
     2521 gaacgttgcc ggcagcgcac cggactgctg aagcacgccg gcattgccga gggcgacgtt
     2581 gggggtcagc acgatccagc cggacgccca gctgcccgta tcgcaggtcg ggggagcggt
     2641 cgccagagtg gaggcgcaga tcgtgatgcc ggcgccgccc gcagtcggca cgtcttcctt
     2701 gatggcctcg ctgcgcgcgt agttcagagc gaccaccaga ctgctcgcgg cgttggaggc
     2761 ctggtaggtc tgcacgtagg tacgcatcgc aggcactgcg atcgccgcca gaatgcccgc
     2821 gataccaatg gtcacgagca gctccagcag gctgaaccct gaagtccttg tgacggcacc
     2881 cgtgccattc agtcgaattg ccatgacaac accttacctc cggtggcttg cggcaatctg
     2941 tcgatcacag caccgctttc catcgcttgt agaattgaga ctcaagtcac atttccgccc
     3001 tgcggcgtaa agcgtacgcg catcctcgcc cccgtattgc ccgctccgcc ctcgatgctc
     3061 agggtagccc gatgtacccg cgcaatctcc tccacgatcg ccaggcccag gccgcatccg
     3121 ggcgctgccg atcccgaagg ccggtagaag cgctcgcgca cgcgccggcg ctctgattcc
     3181 ggaatgcccg gcccgtcgtc ttcgacttcg acataagggg cccgttgcgg acccaggccg
     3241 caacgcaccg tgacatgccc gccgcgaggc gtgtatttca aggcattgtc cagcaggttg
     3301 cccgcgaggt cctgcagcat ccagatgtcg ccctggaggg tggcgtcctg cagttcagcg
     3361 cccagatcca gacccaggct ctcggcacga tccacgttgc gaccgatgag ctcttccagc
     3421 atcggacgca gcgcgaccgg ttcgaatttc tccgactgca gcgaggactg ctcggtcctt
     3481 gccagcgaca gcagctgatt ggcggagcgc gccacccgcg cgatgccctg attgatcgtg
     3541 gacagctccg gtgccagcgc ggcggccccg ggattgtgca gcagcagctc gatctgcgcc
     3601 gacaggcccg cgatcggcgt gcgcaactgg tgcgccgcat cggccacgaa tcggcgctgc
     3661 gaggcggcgg catcctgcag cagttccagc agctgattga acgcgcgcac cagcggacgc
     3721 acttctccca caacctcggt ttcgctgaac gcacgcaatt cgcgcgccgc actcgcctcc
     3781 accacgtcat gcaaccgacg cagtggacgc agtcccacgt acaccgatat ccataccaga
     3841 atcagcgcca gatccagctc cacgaagtcc accgagagct tacccaccaa catggcccgc
     3901 tccgcgcgct cacggcgctg cacggtctcg cccagataaa tcgtgaccgg gcctgcggga
     3961 gtctgactgc gggtgcgcac cacacgtagc gctcggcctg ctgcggacgc gtcgtaatag
     4021 agcggatccg cagccgtgga ccgcgctttc gagaccagcg cggcattgcc cgcgagcaag
     4081 gtaccctcgg gcgagacgat tgcgtacgac tggcggtcga agcggctgcg ttgggctcct
     4141 tcgcgctccg gcgtgagcac gtccagcttg ccgtcctcga ggcggatcag gctggccaag
     4201 gtcagcgccg aattgtccag cgcctgatcg taggagttgc gcgtggcgtc gaccgcaatc
     4261 aggtagtcga tgacaccgcc gccaaacatg acgatcgcca acgccggcaa catcagcagc
     4321 agcagtcggc gcgtcacggt catgatggtt tgagtgccgc accttcatcg agtcgatagc
     4381 ccaagccgcg gatggtgcgg atcacggcgg catcacccag cttggcacgc agacgcgaca
     4441 cctgcgtttc gatggcgttg ttcgacaatt cctgctcacc gctcgccaga tcctgttgca
     4501 ggcgttcctt ggtcaccacg cgtccgatgc tatgcgccag gctctcaagc acgatc
//
LOCUS       DATMRM010000057         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3120851, whole genome shotgun sequence.
ACCESSION   DATMRM010000057 DATMRM010000000
VERSION     DATMRM010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3120851"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..516)
                     /locus_tag="VNZ06_01600"
     CDS             complement(232..516)
                     /locus_tag="VNZ06_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19474.1"
                     /translation="MLALAAWAAAFAAACLSGLGLIAGIAWALLIAAALAWLRWTWLL
                     RLAVCVGAVAVWHWPLVLALLLAVPRAPLLLPGLVSSTLASWRHPRERWS"
     gene            complement(544..1362)
                     /locus_tag="VNZ06_01605"
     CDS             complement(544..1362)
                     /locus_tag="VNZ06_01605"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HWW19475.1"
                     /translation="MLSSHSLSDTTVHDARKEIRRSRAAVRLLRVALGRARFRRENAR
                     LRDAGRALNEARDAKVLVSTLDSLRKCHPQLERDHAFAALSERLREQQRTSRRHLNSA
                     KTPSIAAACRTLEQTQFSASRWPVGNGGWRALGPAFRRVYAAGRVAARKSRTRPDEHQ
                     MHEWRKQVKYLRHALQVFAPLRPSKLKKHARLARRLAESLGDGHDLALLRDSAVSGAN
                     RPALKLKPLLAAIDARRRALRKQSLELAERVYAHSPHQMDKRLQRYWHRWRDQD"
     gene            1597..2115
                     /locus_tag="VNZ06_01610"
     CDS             1597..2115
                     /locus_tag="VNZ06_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19476.1"
                     /translation="MPERKRFPGSAQIIDLARARAVRAQLRRPRRTSARSPRSVLVVG
                     LAIGALAGACLTRLLTGDELTQYRHGTLLAQGALASALNEQLVGHVPQSTSIRVTATY
                     RGRGGNYCRTFSVTGAASLTGLACRNGAQWQLQTLLSGANSSSGLELNKYFSAAPLSS
                     VAEAQLRSHDWQ"
     gene            complement(2131..>2704)
                     /locus_tag="VNZ06_01615"
     CDS             complement(2131..>2704)
                     /locus_tag="VNZ06_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19477.1"
                     /translation="QAAFLLMMTWVNTVAYFMQTDLIARRFSALDARTQAIADIDLMV
                     NVSSAVILALGLGPILRRFGVKAGLILNPIFMVPAFVAVLVWPSVLTIQLVQVVRRVG
                     QYAVARPSREICFTVVEQNSRYRAKNVIDTVVYRFGDLSSAWVQTALRVAGFGVVGAV
                     ALGVAASAFWGWTATRLSTQYEMLRAAPAA"
BASE COUNT          490 a          928 c          822 g          464 t
ORIGIN      
        1 atcattctca cgcttcgcat atccaaactc ccctatgttt cgtaccgagg tagtccctgc
       61 gctcacagaa caccggtgac gcgccggaag tttcagctag attggcacac tcctggatcc
      121 gtccactaat tatctttaga agttaatagc ttaggaagct cccgtaggat ttcacatcag
      181 atgaacgcac cattgcgggc agtcggtcgg tggcatcgcg acctcgcctg gttacgacca
      241 gcgctcgcgc ggatggcgcc agcttgccag cgtgctggac accaggccgg gcagtagcag
      301 cggtgcgcgc ggcactgcta gcaacagcgc cagaacaagc ggccaatgcc acaccgcgac
      361 ggcaccgacg cacaccgcca ggcgcagcaa ccaggtccat cgcagccacg ccaacgccgc
      421 ggcgatcagc agcgcccagg cgataccggc gatcaggccc aacccgctca ggcaggccgc
      481 agcaaaggct gcggcccacg ctgccagggc cagcatgagg ctgactcgta tcgcgagctt
      541 cacttaatct tgatcgcgcc agcgatgcca gtagcgttgc aggcgtttat ccatttggtg
      601 tggggagtgc gcatagacgc gttcggcaag ttccagcgac tgcttgcgca gtgcgcgccg
      661 gcgcgcatcg atcgctgcca gcaacggttt cagttttagc gcgggccggt tggcgcccga
      721 caccgcggaa tcgcgcagca gcgccagatc gtggccatcg cccaggctct cggcaagccg
      781 gcgcgccagc cgcgcgtgct tcttgagttt ggatggtcgc agcggcgcaa atacctgcag
      841 cgcatgccgc aggtacttca cctgcttgcg ccactcgtgc atctggtgct cgtcgggccg
      901 ggtacgtgat ttgcgcgctg cgacgcgtcc ggcagcgtag acgcgcctga acgccgggcc
      961 gagtgcacgc cagcccccgt ttccgaccgg ccagcggctc gcactgaact gcgtctgctc
     1021 aagcgtacgg caagccgctg cgatcgacgg cgtcttcgcc gagttcagat gcctgcggct
     1081 ggtgcgttgc tgttcgcgca aacgctccga cagcgccgca aacgcgtggt cacgctccag
     1141 ctgcggatga cacttacgca atgaatccag tgtactcacc agtactttgg cgtcgcgcgc
     1201 ctcgttcaga gcgcggcccg cgtcgcgcaa gcgcgcgttt tcgcgacgaa atcgtgctct
     1261 tcccagagcg acgcgcagca atcgaaccgc agcccgggat cttctgatct ctttgcgtgc
     1321 atcgtgcaca gtcgtatcac tcagcgaatg ggagctgagc atacggcgtg ctctgagaat
     1381 gccctgtcgc gccacgcgcc gcatttccgc ggttgcgtcg cggccggcgc gaatcccctg
     1441 acttgccata gtgcgatgct acggcaggca cccctgctcg tacaccctcc ctctgccgcc
     1501 gggcgcctac ctacaaaaag gcccaaggca cttgccctga cgcgactcgg cgcggatatt
     1561 actccgatca tgaagagtca ctagccagct cgccacgtgc ccgaacgcaa acgctttccg
     1621 ggttcagccc agatcatcga cctggctcgc gcgcgcgccg tgcgtgcgca gcttcgccgg
     1681 cctcgtcgca cgagcgcccg ctcgccgcgt tccgtactgg tggtagggct tgcaatcggc
     1741 gcgctcgccg gggcctgcct gacccgcctg ttgacgggcg acgagctgac gcagtatcgg
     1801 cacggcactt tgctggccca gggtgccctg gccagtgctt tgaacgagca actggtcggc
     1861 catgtgcccc aaagcacctc catccgcgtg acagcaacgt accgcggccg gggtggtaac
     1921 tactgccgca cgttcagcgt gaccggggct gcctctttga cggggctggc gtgtcgcaat
     1981 ggcgcccagt ggcagcttca gacgctgctg agcggcgcca actcctcctc agggctggaa
     2041 ctcaacaagt acttcagcgc tgcgccgctg agctcggttg ccgaagcgca gctgcgctcg
     2101 cacgactggc aatagtcgtc cgatcgcggt tcaggcggcc ggcgccgcgc gcagcatctc
     2161 gtattgcgtg gacaggcggg tagcggtcca gccccagaat gcggaggccg cgactcccag
     2221 agccaccgcg cccacgacac caaaaccggc gacacgcagg gcggtctgca cccacgccga
     2281 actcagatca ccgaaccgat agaccaccgt gtcgatgaca ttcttggcgc ggtagcgact
     2341 gttctgctcg accacggtga agcagatttc ccggctcggg cgtgcgaccg cgtattggcc
     2401 gactcgccgc accacctgca ccagctgaat cgtgagcacg gatggccaca ccagcaccgc
     2461 aacaaacgct ggcaccatga agatcggatt gaggatcagc ccggccttga ctccgaatcg
     2521 gcgcaggatc ggtcccagcc cgagcgccag tatcaccgcg ctcgaaacgt tcaccatcag
     2581 atcgatgtcg gcgatcgcct gcgtgcgcgc atcgagcgcc gagaacctgc gcgcgatcag
     2641 gtcggtctgc atgaagtacg ccaccgtgtt gacccaggtc atcatcagca gaaaggcggc
     2701 ctgc
//
LOCUS       DATMRM010000058        17681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2192362, whole genome shotgun sequence.
ACCESSION   DATMRM010000058 DATMRM010000000
VERSION     DATMRM010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17681
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2192362"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VNZ06_01620"
     CDS             <1..891
                     /locus_tag="VNZ06_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWW19478.1"
                     /translation="RAGDVIPEIVRVVAPQQRSKDARRPRLPEKCPVCGSPVVRLEGE
                     AAARCTGGFTCSAQRKEALRHFASRRALDIEGLGDKLIDQMVEQDILHGPSDIYALTQ
                     HQLMDLERMGEKSAANLVAAIERSTRTTLPRLLNGLGIPGVGESTAKALADHFGSLDA
                     LQAATPEQIQQTPDVGPVVGASIAGFFADSRHQNELKRLRGFGLRWQEGAPKRTSQTS
                     RLPLAGLTVVLTGTLDGISRDEASDQLTALGAKVSASVSKKTSYVIAGAEAGSKLTRA
                     QELGIPVLDERGLQELLSRK"
     gene            complement(901..1794)
                     /locus_tag="VNZ06_01625"
     CDS             complement(901..1794)
                     /locus_tag="VNZ06_01625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWW19479.1"
                     /translation="MKILNGLPFAALLLLTACNQATTPADTASAKPVDKPVATVNGEV
                     IGHNMYEFYVKNTVGKPSSELTAEQRGQALDNLVRGEVIAQEAVKQGLDKSGDASSEL
                     ALSRLQILQQAGALHYLDDKKPSEAEEKAEYDSQVAQMPKTEYHARHILVKTPEEAQK
                     IIDQLKKGAKFEELAKKFSTDSSKDQGGDLGFFSPQTMVPQFSAAVEGLKKGEYTQTP
                     VQTQFGYHVIQLIETREKPVPPFDQVKDRVAQILQQKKFRDYEDDLIKQAKIEKYLDG
                     GSGSSSGAGAASPSPASKPGQ"
     gene            complement(1829..2113)
                     /locus_tag="VNZ06_01630"
     CDS             complement(1829..2113)
                     /locus_tag="VNZ06_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HWW19480.1"
                     /translation="MSPASNPIAERIEQLLQQRLKPAHLEVRDDSAAHAGHASAGGRG
                     HFHLKIVSTCFEGLKPLQRHRLVNQTLAALFETDVHALGLETLTPAEFKK"
     gene            complement(2115..2681)
                     /locus_tag="VNZ06_01635"
     CDS             complement(2115..2681)
                     /locus_tag="VNZ06_01635"
                     /inference="COORDINATES: protein motif:HMM:NF016189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane-spanning protein YciB"
                     /protein_id="HWW19481.1"
                     /translation="MLSVQRMQPLLDLAPLLAFLLAYYLGGIYVATMVLMASMVALVL
                     LDLVRQRGVSGMHLISTVLVLLLGSATLILRDPRFLKWKPTIFLWLIALTSLGSTWIG
                     RSPLSQRLLAQVVPGSEALPRALWLRLNWIWTLFYAALGALNLWVAYSMSERFWVNFK
                     VIGLSAAFVVFAMAQALWLSSRIEAQTA"
     gene            2720..3364
                     /locus_tag="VNZ06_01640"
     CDS             2720..3364
                     /locus_tag="VNZ06_01640"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HWW19482.1"
                     /translation="MAQYFELHRQNPQLRLIRRAAEIVRGGGVIAYPTDSCYALGCHI
                     GDAAALERLRRIRMADRNHHFTLVCRDLSEIARYARVDTWQFRMLKHATPGPFTFLLQ
                     ATRETPRRLQHPRRRTIGIRVPDHIVPQMLLTELGEPLMSSTLLLPGDELPLTDGDQI
                     RARLEHQLDAILDGGHCGIEPTTVVDLAVSPPLVVRAGRGKLAGTEWEVVGNTA"
     gene            3407..4621
                     /locus_tag="VNZ06_01645"
     CDS             3407..4621
                     /locus_tag="VNZ06_01645"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HWW19483.1"
                     /translation="MSAVLPGKRVLSGMRPTGALHLGNYHGALRNWVELQYQYECFFF
                     VADWHALTTGYEDTAGLPQAIREVVIDWLAAGLNPGVATMFVQSYVPEHAELHLLLSM
                     ITPLGWLERVPTYKDQQEQLQDKDLATYGFLGYPLLQSADILLYRASSVPVGEDQAAH
                     VEVTREIARRFNHLYGREPDFEQKAERAIKELGGRNITLYRQLRRRFQETGDQVAVER
                     ARALVESNTRITVADRERLLGYLEGSSVSILAEPQALLTAAPRVPGLDGRKMSKSYGN
                     TIGMREDPDSVTAKLKAMQTDPARVRRTDPGDPEKCPVWDLHKLYSDEATRKWVNEGC
                     RSAGIGCLDCKKPLIERIVEEVSGMRKRAQEFQDSPDMLNNILVEGSEKAREAARQTL
                     DDVRRAMHLRSG"
     gene            4638..5573
                     /locus_tag="VNZ06_01650"
     CDS             4638..5573
                     /locus_tag="VNZ06_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HWW19484.1"
                     /translation="MKPDLKIVVSNPSPAAPVAASPEQVEMPFAVVNGEAITQLPRDL
                     YIPPQALEIFLEAFEGPLDLLLYLIRRQNVDILNIPIAEITLQYTQYIELMQELQLEL
                     AGEYLLMAATLAEIKSRMLLPRPAGQGDGSEADPRAELVRRLQEYERYKRAALDIDTM
                     PRLERDVWQASAELRDRPVVRALPQVTLQEMLLAFKEVAVRATMFAHHHVTREPLSVR
                     ERMSNILAALEQGSFLEFQALFSIEEGRAGVTVAFIAILELMREGLIDIMQVEPYAPL
                     HVRAAAANRTLHLVADNAQPANDAPGEPVEALRND"
     gene            5566..6294
                     /gene="scpB"
                     /locus_tag="VNZ06_01655"
     CDS             5566..6294
                     /gene="scpB"
                     /locus_tag="VNZ06_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HWW19485.1"
                     /translation="MIDTEVRNVVEAALFAAGKSLTINELSQLFDESDRPTPNGIREA
                     LEQLTQEFASRPVEIKETGAGFRVQVRRQYAPAVAKLWPERPQRYSRALLETLALIAY
                     RQPITRAEIESVRGVTVNPNITKTLLERQWIRVVGHRDLPGRPELLGTTREFLEHFSL
                     RSLDELPPLSELRSLEQLDPQLDLADAADAGADQANVASASLPTPDEQIEEEAAEADS
                     DPDEFSAAPASSSGLVAAPPDDTH"
     gene            6304..7065
                     /locus_tag="VNZ06_01660"
     CDS             6304..7065
                     /locus_tag="VNZ06_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HWW19486.1"
                     /translation="MTSVTGERLQKVLAQLGLGSRREAEAWIRAGRLTVNGRPAVLGM
                     RVGSQDQVKLDGRTVRRTLAAAAAPVFLCHRSPGEPLLPARGAAATAEAVAVSIAERL
                     PRRAGRRFISISPMPNVDGGLELLTADGAIAERLQRAVHAMEAEFSLRVRGELSETQQ
                     QAIRDGALDSGARVQVLDLEANGGEGSNRWYRLLAQGASGNQIRQLLERQAVTLARVL
                     RTRLGTLELPRTLARSHWQQLTTEQLDSLTHAPRA"
     gene            complement(7123..7881)
                     /locus_tag="VNZ06_01665"
     CDS             complement(7123..7881)
                     /locus_tag="VNZ06_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009387635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HWW19487.1"
                     /translation="MPDISQVAPDELEGRVIAVTGAGDGIGRAVALAAAGHGAEVILI
                     GRTVAKLEAVHASIAALGKAAASIAPLNLEKAIARDYDQLAEALRQRYGRLDGLAHLA
                     GILGSLTPIEQYDVPLWCRVLHVNLTAAFALTQVLMPALRASHDASVIFTSSAVAQAP
                     RAYWGAYAVSKSGVETLARVLADELEHYGNLRVNTLDPGPVATRMRRQAFPAEDPSTL
                     KIPEQIVGAYLWLLGRAARGVNGQSLACQPDGGK"
     gene            complement(7890..8567)
                     /locus_tag="VNZ06_01670"
     CDS             complement(7890..8567)
                     /locus_tag="VNZ06_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19488.1"
                     /translation="MSEAQHPAAPAPGSRRYFTLLYTPEARREQLHTLLALADEIGAA
                     PLASADHSVAHVRLEWWRQEAERFARGEPQHPWLRALLREHPAAASLDLQSLVQAAAL
                     DLATHTLASHSAHALRRALFELAAQALCAPPLARELARSVGELGANLQRLESDPSDAQ
                     ARGALQQQLQQIGDAPQRSLTPLLVWLALAAQRPQRRAALLESLTNNLIAWSAARRAA
                     RGRFRLQ"
     gene            complement(8564..9652)
                     /locus_tag="VNZ06_01675"
     CDS             complement(8564..9652)
                     /locus_tag="VNZ06_01675"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HWW19489.1"
                     /translation="MNWPLAVSALAGALIGVIVTLLIGRRLLRELLGREHASIDGAWR
                     ERERALQAVVEPLRSALHRTEKQVEALERDRRDAFTTLRTQIESLSRGQSLLQQETRN
                     LVTALRRPEVRGRWGELTLRRVVELAGLSEHCDFIEQTSISAAQGVLRPDLVVQMPEG
                     RQLVVDAKTPLDAYLDAIDASSEQARAQALNKHASQLEARVRDLAGKAYWAQFERCPE
                     FAVLFLPGDQFLSSALSSRPDLLENAIRSNVIIATPSTLIALLKTIAYGWRQAAVAEN
                     AAQIRDLGQELHRRLASFVEHLDRSGRALSRAVESYNAAFGSLERQVLPSARRFTELG
                     ATCADPITAPEPIEELVRALSGERERHP"
     gene            complement(9659..10354)
                     /locus_tag="VNZ06_01680"
     CDS             complement(9659..10354)
                     /locus_tag="VNZ06_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWW19490.1"
                     /translation="MNPGTPLGAALFDLDGTLLDTAPDLAYALNALRVECALPQLPFA
                     PIRAQVSNGAAAVLRLGFPNAGEERFATLRARFLQLYRSHLARETRLFPGFDEVLERL
                     EAHGIPWGIITNKPASFTEPLLQELGLFERAGCVLSGDSLPVTKPDPLPLITAARKIG
                     LPPNRCLYLGDALRDAQAARAAGMTSLGARFGYIAAGEDVSGWPVDAWLDEPRELLAW
                     VGLAAPHLPRAGA"
     gene            complement(10351..11076)
                     /gene="ubiG"
                     /locus_tag="VNZ06_01685"
     CDS             complement(10351..11076)
                     /gene="ubiG"
                     /locus_tag="VNZ06_01685"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: protein motif:HMM:TIGR01983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HWW19491.1"
                     /translation="MNQPSAQAVAIDAEVRKFDALAHEFWDARGAFQALHTLNPVRLD
                     YVAARAKLAQQRVLDVGCGGGLLAEALSRSGAIVTGIDLAPAMIEVARLHAHESQLRI
                     DYRLLSAEQLAQRCEQPFDVITCMEMIEHVPDPAGLLLAIARLLRPGGQLFLSTINRN
                     LRSFLLAIVGAEYVLKLLPRGTHEYARLLRPSELARHARAANLSLRDVRGIEFNPFLR
                     SARLTGDTGVNYLAHFESEAELK"
     gene            11201..13792
                     /gene="gyrA"
                     /locus_tag="VNZ06_01690"
     CDS             11201..13792
                     /gene="gyrA"
                     /locus_tag="VNZ06_01690"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWW19492.1"
                     /translation="MPVSLEDEMKQSYLDYAMSVIVGRALPDVRDGLKPVHRRVLHAM
                     RELGNDYNRAYKKSARVVGDVIGKYHPHGESAVYDTIVRMAQSFSMRYMLVDGQGNFG
                     SVDGDAPAAMRYTEVRMSKLAHELLADIDKDTVDVVPNYDESETEPSVLPTRVPNLLV
                     NGSSGIAVGMATNIPPHNLTEISNALLALIDDPSISLAGLMQHVPGPDFPTAGIINGA
                     QEIAAAYKTGRGRLSIRARAHIEDMDSASRQAIIVTELPYQVNKARLLERMAELVRNK
                     QIDGISELRDESDKDGMRVVIELKRGEVAEIVLNNLYAQTPMETVFGINMVALLDGQP
                     RLMSLREMLDAFVRHRREVITRRTIFELRKARERAHILEGLAVALANIDEVIALIKSS
                     PTPAEARTAMLARVFSPGAVAAMLGTAGAAQTRPDGLSVALGLTATGYRLSEEQAQAI
                     LDMRLHRLTGLEQDKIVEEYRTLLAQIADLADILARPERLMQVVREEIVQIRDSYGDA
                     RRTEISRDHLNLSTEDLIEPQDMVVTLSHAGYAKAQPVSDYQAQRRGGRGRAATAVKD
                     EDFIEKFFVAHTHDTLLCFSNRGKIYWLRVFELPQGGFGSRGKPVVNLLPLEEGEKIS
                     AVLPVKQFDEQHFVFMATSSGTVKKTPLAAFSRPRPSGIIAVDLRGDDHLVGVSLTDG
                     NREIMLVTSGGKSIRFHEDEVRPMGREAAGVIGVRLGTGQRVISLLVLGEGLILTASE
                     SGYGKLTPLDDFPSHGRGAQGVIALQTTERNGATVAALQVQAGDELMLISSTGTLVRT
                     PVADISIVGRNTQGVRLIRLGDGERLTGIERVAAGLENGDDVVGPGSDDSSTLGETGS
                     DESSPVQ"
     gene            13837..14913
                     /gene="serC"
                     /locus_tag="VNZ06_01695"
     CDS             13837..14913
                     /gene="serC"
                     /locus_tag="VNZ06_01695"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HWW19493.1"
                     /translation="MRVFNFAAGPAALPLEVLERARDEITDWNGSGMSVMEVSHRGKA
                     FIAVAAQAEADLRELLSIPKHYKVLFVQGGATGMFSAVPMNLTQADSVIDLVNTGSWS
                     KKALGEARRYCKVSVAADAADCNYTSVPPQSAWKLTPNAAYLHYCANETIGGVEFAFI
                     PQVPVPLVSDMSSTILSRPLDVSRFGLIYAGAQKNIGPAGICVVIVREDLIGKARPGT
                     PSVWDFKALADEGSMLNTPPTFAWYLAGLVFQWLKAQGGLAAMAERNRSKAQLLYHTI
                     DSNPFYRNPVEPSSRSWMNVPFLLAKPQLEASFLSEAKQAGLMNLEGHRSVGGMRASL
                     YNATTLQAVQALTDFMQQFARRHG"
     gene            14930..16111
                     /locus_tag="VNZ06_01700"
     CDS             14930..16111
                     /locus_tag="VNZ06_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HWW19494.1"
                     /translation="MGFRILTLNNISMRGLERMPRETYEVSAQIGEPHAVMVRSADMH
                     KLTIASSVLAVARAGAGVNNIPVDALSKRGIPVFNAPGANANAVKELVLSGLFLAARN
                     ICQAWVYTRGLSGDDRTLDTTVEANKKNYVGFELPGRTLGVIGLGAVGVLVANAAEAL
                     GMKVLGYDPQITVARAWQLNASVQQALSLDDLFARSDMISVHVPLLDATRGLVNAARL
                     GLMRRGGTVLNFARAPIVNEQDMVAALNAGQLHSYICDFPTRALKDHPRAITLPHLGA
                     STVEAEDNCAIMAADTLREFLEQGHIRNCVNFPEAVLPREVGSIRLAIANANVPNMVG
                     QISTCLADAGLNIAELLNRSLREYAYTLIDAEGSADEALLQRIRAIDGVLSARLVRVA
                     D"
     gene            16117..17292
                     /gene="pheA"
                     /locus_tag="VNZ06_01705"
     CDS             16117..17292
                     /gene="pheA"
                     /locus_tag="VNZ06_01705"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017677363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HWW19495.1"
                     /translation="MARARDRAGSSAKRRVSSAKSRAATPSAAPPAADLQTLRARIDS
                     VDQRLHALINERARLAQQAGISKHKDGHTVDFYRPEREAEVLRMALERNHGPLRNEEI
                     VRLFREIMSACLAQEEPLKVAFLGPEGTFTQQAVLKHFGHSVRALALPTVDEVFQEVE
                     GAAADFGVVAIENSSEGTITNTLDRFLASSLHICGEVELRVHHCLMGKMDSFARVLRV
                     CAHGQALAQSRGWLNDHLPDAERVPVASNAEGARRARDEQGTAAIAGEIAAEVYGLNV
                     LAAEIEDRPDNSTRFLVLGRKILRTSGADRTTLLLSAGDTQKPGALQRLLEPLARHRI
                     SMSRIESRPSRRRKWDYVFFIDIDGHAEDPNVRKALSALKKRASLFRILGSYPRAVL"
     gene            17289..>17681
                     /locus_tag="VNZ06_01710"
     CDS             17289..>17681
                     /locus_tag="VNZ06_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWW19496.1"
                     /translation="MSAAGPGRLAVPAVRGLSPYIPGKPIAELERELGIHDIIKLASN
                     ENPYGPSPKVRAAIQRAIEDVWLYPDGSCYELKQALAQHLNVDPVCITVGNGSNDLLM
                     LLAEAFLNADTHAVHSQYGFAIYPLVIRE"
BASE COUNT         3117 a         5708 c         5891 g         2965 t
ORIGIN      
        1 cgtgccggcg atgtcatccc ggagatcgtg cgggtggtgg ccccgcagca gcgctcgaag
       61 gatgccaggc gcccgaggct gcccgaaaaa tgcccggtgt gcggctcgcc ggtggtacgg
      121 ctggaaggcg aggcggccgc gcgctgcacc ggaggcttca cctgctccgc gcagcgcaag
      181 gaggcactgc gacacttcgc gagccgccgc gcgctcgaca tcgagggcct gggggataag
      241 ctgatcgatc agatggtcga gcaggacatt ctgcacggcc cgagcgatat ctatgcgctc
      301 acgcagcacc agctgatgga tctggagcgc atgggcgaga aatcggctgc gaacctggtg
      361 gccgcgatcg agcgcagcac gcgcacgacg ttgccgcgac tgctgaacgg actggggatt
      421 ccgggtgtgg gcgagagcac ggccaaggcc ctggccgatc acttcggttc gctcgatgcg
      481 ctacaggccg caacgcccga gcagatccag cagacaccgg atgtggggcc cgtggtcggt
      541 gcaagcatcg ccggtttctt cgcggattca cgtcatcaga acgaactgaa acgactgcgc
      601 gggttcggcc tgcgctggca agaaggcgcg ccgaagcgca ccagccagac gagccgactg
      661 ccgctggcgg gcctgaccgt ggtgctgacc gggacactgg atggcatcag ccgcgatgag
      721 gcgagcgatc agctgaccgc gctcggtgca aaggtcagcg cttcggtctc gaagaagacc
      781 agctacgtga tcgcgggggc cgaggccggc tccaagctca cgcgcgccca ggaactggga
      841 atcccagtcc tggacgagcg cgggctgcag gaactgctga gccggaagta gcctgcggcg
      901 ttattgtccg ggcttgctgg cgggggacgg gctggccgcg cccgcaccgc tggaggatcc
      961 cgagccgccg tcgaggtact tctcgatctt cgcctgcttg atcaggtcgt cctcgtagtc
     1021 gcggaatttc ttctgctgca ggatctgcgc aacacgatcc ttgacctgat cgaacggcgg
     1081 taccggcttc tcgcgcgtct cgatcagctg aatcacgtga tagccgaact gcgtctgcac
     1141 cggagtctgc gtgtattcgc ctttcttcag accctcaacc gcggcagaga actgcggcac
     1201 catggtctgc ggtgagaaga aaccgaggtc accgccctga tctttggagg agtcggtcga
     1261 aaatttcttg gcgagctctt cgaacttggc gcccttcttg agctggtcga tgatcttctg
     1321 cgcctcctcc ggagtcttta ccaggatatg gcgtgcgtgg tattcggtct tcggcatctg
     1381 cgcgacctgc gagtcgtact ccgctttctc ctccgcctcg ctcggcttct tgtcatccag
     1441 gtagtgcagg gcgccggcct gctgcaggat ctgcagccgc gacagcgcga gttccgatga
     1501 ggcatcgccg ctcttgtcca gaccctgctt cacggcctcc tgcgcaatca cttcgccgcg
     1561 caccagatta tcgagcgcct gtccgcgctg ttcggcggtc agttcgctgg aaggcttgcc
     1621 caccgtgttc ttgacgtaga actcgtacat gttgtgtccg atcacctcgc cgttcaccgt
     1681 tgccaccggt ttgtccaccg gctttgcgga cgcagtgtcg gcaggcgtcg tcgcctggtt
     1741 gcaggcagtc agcagcagta gcgccgcgaa cggcagtcca tttaggattt tcatcagata
     1801 tctcattgtg ttacgttaat gacttaaact atttcttgaa ttcagcgggg gtcagggttt
     1861 ccagccccag cgcatggacg tcggtctcga acagcgcggc cagtgtctgg ttgaccagcc
     1921 ggtgtcgctg caacggcttc aatccctcaa agcaggtgga cacgatcttc aggtgaaagt
     1981 gccccctgcc tccagcgctg gcgtgaccgg catgcgcggc gctgtcgtcg cgcacttcca
     2041 gatgggcggg cttgagccgc tgctgcaaca gctgctcgat gcgctcagcg atcgggttgg
     2101 aagccggtga catatcaggc cgtctgcgcc tcgatgcgtg aggacagcca cagcgcttgc
     2161 gccatcgcga acacgacgaa cgcggcgctc aggccgatga ccttgaagtt gacccagaag
     2221 cgctcgctca tcgagtaggc cacccacaga ttgagcgctc ccagtgccgc atagaacagc
     2281 gtccagatcc agttcagccg cagccacagg gcacgcggca gcgcctcgct cccgggcacg
     2341 acctgagcca gcagacgctg tgacagcggg ctgcggccga tccaggtgct gcccaggctg
     2401 gtcaacgcaa tgagccataa aaatatcgtc ggcttccact tcagaaagcg cggatcgcgc
     2461 aggatcagcg tagcgctgcc gagcagcagc accagcacgg tgctgatcag atgcatcccg
     2521 gagacgcctc gctggcgcac cagatccagc agcaccagtg ccaccatcga tgccatcagc
     2581 accatggtcg ccacgtagat accgcccagg tagtacgcca gcagaaacgc cagcaggggc
     2641 gccagatcga gcaggggctg catccgttga acgctgagca cggctgggtt ggcgattgag
     2701 ggccgctatg atagcggcca tggctcaata cttcgagttg caccgtcaga acccgcaatt
     2761 gcggctgatc cgccgggcgg ccgaaatcgt gcgcggcggt ggtgtcatcg cctatcccac
     2821 cgattcctgc tatgcgctgg gctgccacat cggggatgcg gcggcgctgg agcgcctgcg
     2881 ccgcatccgc atggccgatc gcaaccatca cttcaccctg gtgtgccggg acttgagtga
     2941 gatcgcgcgc tacgcccgcg tggacacctg gcagttccgc atgctcaaac acgccactcc
     3001 cggaccgttc acgtttctgc tgcaggcgac acgcgagacg ccgcggcggc tgcagcatcc
     3061 gcgccgccgt acgatcggca tccgcgtgcc cgatcacatc gtcccgcaga tgctgctgac
     3121 cgaactcggt gagccgctga tgagctcgac cctgctgctg ccgggagacg agctgccgtt
     3181 gaccgatggc gatcagatac gagcgcggct cgagcaccag ctcgatgcca tcctggatgg
     3241 cggacattgc ggaatcgagc cgactaccgt ggtcgatctg gcggtttccc cgccgctggt
     3301 ggtgcgcgcc ggtcggggca agctcgccgg cactgagtgg gaagtggtcg gcaatacggc
     3361 ctgagaaggc cgtataatca atgtcagaca tgaatctgct cacaggatga gtgcggtcct
     3421 cccaggcaaa cgcgtgctgt caggcatgcg tccgaccggt gccctccatc tcgggaatta
     3481 tcacggcgcg ctgcgtaact gggtcgagct gcaatatcag tacgagtgct tcttcttcgt
     3541 cgctgactgg cacgcactga ccacgggcta tgaggatacg gcgggcctgc cgcaggcgat
     3601 ccgcgaagtg gtcatcgact ggctggccgc gggactcaat ccgggcgttg ccaccatgtt
     3661 cgtgcagtcc tatgtgcccg aacacgccga actgcatctg ctgttgtcga tgatcacccc
     3721 gctgggctgg ctcgagcgcg tgccgaccta taaggaccag caggagcagc tgcaggacaa
     3781 ggacctcgcc acctacggct ttctcggcta cccgctgctg cagagcgcgg acatcctgct
     3841 gtaccgggcg agctcggtgc cggtcggcga ggatcaggcc gcgcacgtgg aggtcacacg
     3901 cgagatcgcc cggcgtttca atcatctcta cggtcgcgag ccggatttcg agcagaaggc
     3961 cgaacgcgca atcaaggagc ttggcggccg caacatcacg ctctaccgcc agctgcggcg
     4021 ccggttccag gagaccggtg atcaggtcgc ggtcgagcgc gcccgcgcgc tggtggagag
     4081 caatacccgc atcacggtgg cggatcgcga gcgcctgctc ggctatctgg aaggctcctc
     4141 ggtgagcatc ctggccgagc cgcaggcgct gctgaccgcg gcgccaagag tgcccgggct
     4201 ggacggccgc aagatgtcca agtcctatgg caataccatc ggcatgcgcg aggatccgga
     4261 ttcggtcacc gccaaactga aggccatgca gaccgatccg gcgcgcgtgc gccgcaccga
     4321 tcccggtgat ccggagaagt gtccggtgtg ggatctgcac aagctctatt cggatgaggc
     4381 cacacgcaag tgggtcaacg agggctgtcg cagcgccggc atcggttgcc tggactgcaa
     4441 gaagccgctg atcgagcgca tcgtcgaaga ggtgagcggc atgcgcaaac gggcgcagga
     4501 gtttcaggac agtcctgaca tgctcaacaa cattctggtc gaaggcagcg agaaggcgcg
     4561 tgaagcggct cgccagaccc tggatgacgt gcggcgcgcg atgcatttgc ggtcgggatg
     4621 atttacaagg aggtatggtg aaaccggatc tgaagatcgt ggtgtccaac ccttcgcctg
     4681 ctgcgcccgt ggcggcatcc cctgaacagg tggaaatgcc gtttgccgtg gtcaacggcg
     4741 aggcgatcac ccagctgccg cgcgacctgt acatcccgcc gcaggcgctg gagatctttc
     4801 tcgaggcgtt cgagggcccg ctcgatctgc tgctgtatct gatccggcgt cagaacgtgg
     4861 acatcctcaa tatcccgatc gccgaaatca cgctgcaata cacgcagtac atcgaactga
     4921 tgcaggagct gcagctcgaa ctcgccggcg agtacctgct gatggcggcg acactggcgg
     4981 agatcaaatc ccgcatgctg ctgccgcgac ccgcgggcca gggcgatggc agcgaagcgg
     5041 acccgcgtgc cgagctggtg cggcgcctgc aggaatacga gcgctacaag cgcgccgcgc
     5101 tggatatcga caccatgcca cggctggagc gcgacgtgtg gcaggccagc gccgagctgc
     5161 gtgaccggcc ggtcgtgcgc gcgctgccgc aggtgacgct gcaggagatg ctgctggcgt
     5221 tcaaggaagt cgcggtacgc gccaccatgt ttgcgcatca tcacgtgacg cgtgagccgc
     5281 tgtcggtgcg cgagcgcatg agcaacattc tcgccgcgct cgagcagggc agctttctgg
     5341 aattccaggc gctgttctcg atcgaggagg gacgcgccgg cgtcacggtg gcgttcatcg
     5401 cgatcctgga gctgatgcgc gaaggcttga tcgacatcat gcaggtcgag ccgtacgcgc
     5461 cgctgcacgt gcgtgccgcg gccgcaaatc gtacactgca cctggtcgcc gacaatgcgc
     5521 agcccgcgaa tgacgcaccg ggcgagcctg tggaggcttt gagaaatgat tgacaccgaa
     5581 gtccgtaatg tcgtcgaggc cgcacttttt gcggctggaa agtcgttgac aatcaatgaa
     5641 ttgtcgcaac tatttgatga atcagataga ccgacgccga atggcatccg cgaagcgctc
     5701 gagcagttga cgcaggagtt tgcttcgcgt ccggtggaga tcaaggagac cggcgccggc
     5761 tttcgcgtgc aggtgcgccg tcagtacgcc ccggcggttg cgaagctgtg gccagagcgg
     5821 ccgcagcgct actcgcgcgc gctgctggag acgctggcgc tgattgccta tcgccagccg
     5881 atcacgcgcg cggaaatcga atcggtgcgc ggcgtcaccg tcaatccgaa catcaccaag
     5941 acgctgctgg agcgacagtg gatccgcgtg gtgggtcatc gcgatctgcc gggtaggccc
     6001 gaactgctcg gcaccacgcg tgaattcctg gagcacttta gtctgcgcag cctcgatgag
     6061 ctgccgccac tgagcgagct gcgctcgctc gagcagctcg acccgcagct ggatctggcg
     6121 gacgccgccg atgccggcgc tgatcaggcc aatgtcgcca gcgcgtcact gccgaccccg
     6181 gatgagcaga tcgaggaaga agccgccgag gcggattcgg atccggacga gttttccgca
     6241 gccccggcca gcagctcagg cctggtggcg gcccctcccg acgacacaca ctagaacgct
     6301 tcaatgactt cggtaacggg cgagcgcctg cagaaggtgc tggcacagct tggactcggc
     6361 tcgcgccgcg aagccgaagc ctggattcgc gccggacgcc tcacggtcaa cggccggccg
     6421 gcggtgctcg gcatgcgtgt cggctcgcag gatcaggtga agctcgatgg ccgcacggtg
     6481 cgccgcacgc tggcggccgc cgccgccccg gtattcctgt gccatcgctc cccgggcgag
     6541 ccactgctgc ccgcgcgggg agcggccgca accgccgagg cggtggctgt cagcatcgcc
     6601 gagcggctgc cacgacgggc ggggcggcgc ttcatcagca tcagtccgat gccgaacgtg
     6661 gatgggggac tggagctgct gaccgctgac ggtgcgatcg ctgagcggct gcagcgcgcg
     6721 gtgcacgcaa tggaagccga attcagtctg cgcgtgcgcg gtgagctgag cgagacgcag
     6781 cagcaggcga tccgtgacgg cgcgctcgac agtggcgcgc gggtgcaggt gctggatctg
     6841 gaggccaacg gcggggaagg cagcaaccgc tggtaccggc tgctcgcgca gggtgccagc
     6901 ggcaaccaga ttcgtcagtt gctcgagcgt caggcggtca cgctggcacg agtcctgcgc
     6961 acgcgtttgg gtacgctcga gctgccgcgc acgctggcgc gcagccactg gcagcagctc
     7021 acgaccgagc agctcgactc gctcacacac gcgccacgcg cttagtcagg gccgccttca
     7081 tgccagtccc tgcccgcagt gggcagcgat gacaaggccg tatcattttc cgccatccgg
     7141 ctggcaggcc agactctggc cgttcacgcc gcgagcggcg cggccaagca gccacaggta
     7201 cgctccgacg atctgctccg gaattttcag ggtcgaagga tcttcggccg ggaaggcctg
     7261 tcgtcgcatg cgcgtggcca ccggtcctgg atcgagtgta ttgacgcgca ggttgccgta
     7321 gtgctcgagc tcatcggcca gtacgcgcgc gagcgtttcc acgcccgatt tcgagaccgc
     7381 gtaagcgccc caataggcgc gtggagcctg tgcaactgcg ctgctggtga agatcaccga
     7441 ggcatcgtgc gaggcgcgca gcgccggcat cagcacctgg gtgagcgcga atgccgcggt
     7501 gagattgacg tgcagcacgc ggcaccacaa cggcacgtcg tactgctcga tcggtgtgag
     7561 gctgccgaga atgccggcga ggtgcgccaa gccgtcgagc cggccatagc gctggcgcag
     7621 cgcctcggca agctgatcgt aatcgcgcgc gatcgccttc tccaggttca gcggcgcgat
     7681 gctggccgcg gctttaccca gcgccgcgat gctcgcgtgc accgcttcga gtttggccac
     7741 cgtgcggccg atcagaatca cttcggcccc gtgcccggcg gccgccagcg ccaccgcgcg
     7801 gccgatcccg tcgccggcac cggtgacggc aatcacgcgt ccttcgagtt cgtcgggcgc
     7861 gacctgagag atatcgggca tgcgggtgtt cattgcaggc gaaaccggcc gcgcgcggca
     7921 cggcgcgcgg cactccaggc gatgaggttg ttggtcagcg attccagcag cgcggcgcgc
     7981 cgctgcgggc gctgcgctgc cagcgcgagc cacaccagca gcggcgtgag cgagcgctga
     8041 ggcgcatccc cgatctgttg cagctgctgc tgcagggcgc cgcgtgcctg cgcgtcggag
     8101 ggatcgctct ccagccgttg cagattcgcg cccagttcac cgacgctgcg cgcaagctca
     8161 cgcgccagcg gtggtgcaca cagcgcctgg gctgccagct cgaacagcgc gcggcgcagt
     8221 gcgtgagcgc tgtgtgaggc gagcgtgtgc gtggccagat ccagcgcggc ggcttgcacc
     8281 agcgactgca gatcgagtga agcggcggcg ggatgttcgc gcagcagcgc gcgcagccac
     8341 gggtgctgcg gctcaccgcg cgcgaaacgc tcagcttcct ggcgccacca ctccagtcgt
     8401 acatgcgcca ccgagtgatc cgcgctcgcg aggggcgcgg cgccgatttc atcggccagt
     8461 gccagcagcg tgtgcagctg ctcgcggcga gcctcgggcg tatagagcag cgtgaagtac
     8521 cgccgtgacc cgggcgccgg cgccgccggg tgctgggcct cgctcatgga tgccgctcgc
     8581 gctcgccgct cagcgctcgc accagctcct cgatcggctc gggcgccgtg atcggatcgg
     8641 cgcaggttgc gccgagttcg gtgaagcggc gcgcgctggg caacacctgg cgctccagtg
     8701 agccgaatgc ggcgttatag gattccaccg cgcgcgacag cgcgcgcccg gagcggtcga
     8761 ggtgttcgac gaaactcgcc agccgacgat gcagctcctg tcccagatcg cggatctggg
     8821 cggcgttttc cgccaccgcc gcctgacgcc agccataggc gatggtcttg agcagcgcga
     8881 tcaacgtcga gggtgtggcg atgatcacgt tcgagcggat cgcgttctcc agcagatccg
     8941 gccgtgaact cagcgccgaa ctcagaaact gatcgcccgg caggaacaat accgcgaatt
     9001 ccgggcagcg ctcaaactgt gcccaatagg ctttgccggc cagatcgcgc acccgtgcct
     9061 cgagctggga ggcgtgtttg ttcagggcct gagcgcgcgc ctgctcgctg ctcgcatcga
     9121 tggcatccag ataggcatcc agcggtgtct tggcatccac caccagctga cgtccttccg
     9181 gcatctgtac caccagatcc ggccgcagca caccctgggc cgcgctgatg ctcgtctgct
     9241 cgatgaaatc gcagtgctcg gacaggcccg ccagctcgac cacgcgacgc agcgtcagct
     9301 ctccccagcg accgcgcact tccgggcggc gcagcgccgt gaccagattg cgcgtctcct
     9361 gctgcagcag cgactgcccg cggctcaagc tctctatctg cgtgcgcagc gtggtgaagg
     9421 catcgcgccg atcgcgctcg agggcttcca cctgtttctc ggttctgtgc agcgcgctgc
     9481 gcaacggctc gaccaccgcc tgcaaggcgc gttcacgctc gcgccacgcg ccatcgatgc
     9541 tcgcgtgctc gcgacccagc agctcacgca gcagccgccg tccgatcagc agtgtcacga
     9601 tcacaccgat cagcgccccg gcgagcgcgc tgacggcgag tggccagttc atgagggctt
     9661 acgctcccgc gcgcggcagg tgcggggccg caaggccgac ccacgccagc agctcacgcg
     9721 gctcgtcgag ccaggcatcg accggccagc cgctgacatc ctcaccggca gcgatgtagc
     9781 cgaagcgtgc acccagtgag gtcatgccgg ccgcgcgtgc cgcctgagcg tcgcgcagcg
     9841 catcccccag atacagacag cgattgggcg gcagaccgat ttttcgcgcc gctgtgatca
     9901 gcggcagtgg atcgggcttg gtgaccggca gactgtcacc cgacagcacg cagcccgcgc
     9961 gttcgaacaa cccaagctcc tgcaggagcg gttcggtgaa cgaggcaggc ttgttggtga
    10021 tgatgcccca cggaatgcca tgcgcttcga gccgttcgag cacctcatcg aagccgggga
    10081 agagtcgggt ctcgcgcgcc aggtgcgagc ggtagagctg caggaagcgc gcgcgcagtg
    10141 tcgcgaaccg ctcctcgccc gcattcggaa agcccagccg cagcaccgcc gccgcgccgt
    10201 tggagacctg cgcgcgaatc ggcgcaaagg gcagctgcgg caaagcgcac tccacccgca
    10261 gcgcattcag cgcgtaagcg aggtcagggg ctgtatcgag cagagtaccg tccagatcaa
    10321 acaaagccgc gcccagcggt gtgccggggt tcacttcagc tcagcctcgc tctcaaagtg
    10381 cgccaggtag ttgacgccgg tgtcgcccgt caggcgagcg ctgcgcagga atgggttgaa
    10441 ctcgataccc ctcacatcgc gcagtgacag gttcgccgcc cgcgcatggc gcgcgagctc
    10501 cgacggtcgc agcagccgtg cgtactcgtg cgtgccgcgc ggcaggagtt tcagcacata
    10561 ttcggcgccg acgatcgcca acagaaacga gcgcagattg cggttgatgg tcgagaggaa
    10621 caactggccg ccgggccgca gcagccttgc gatcgccagc agcaggccgg ccggatcggg
    10681 cacgtgctcg atcatctcca tgcaggtgat cacgtcgaac ggctgctcgc agcgctgcgc
    10741 cagttgttcc gcggacagca gccgatagtc gatcctcagc tggctctcgt gcgcgtgcag
    10801 gcgcgcgacc tcgatcatgg ccggagccag gtcgatgccg gtgacgatgg cgccgcttcg
    10861 gctcaaggct tccgccagca atccgccacc gcagcccaca tccagcacac gctgctgcgc
    10921 gagtttcgcg cgagccgcga catagtccag gcgcaccgga ttgagcgtgt gaagcgcttg
    10981 gaacgcgccg cgcgcgtccc agaattcatg cgccagcgcg tcgaatttac gcacctccgc
    11041 atcgatcgcg accgcttgcg ctgaaggttg attcatggct gcaagtatag ctttgttctg
    11101 ttggatccgg cgactggtgc gagcccattt atgctatgat tttcagctta atttcactca
    11161 cagcagatag gattcagtga ccgatatagc gcgcgaaatc atgcctgtca gtctcgaaga
    11221 cgagatgaaa cagtcctatc tcgactacgc catgagcgtc atcgtcggac gcgcactgcc
    11281 cgatgtccgc gacggtctga agccggtgca ccggcgcgtg ctgcatgcga tgcgcgagct
    11341 cggcaacgac tataaccgcg cctacaagaa gtcggcgcgc gtggtcggcg atgtcatcgg
    11401 caagtaccac ccgcacggcg aatccgcggt ctacgacacc atcgtgcgca tggcgcagtc
    11461 gttttcgatg cgctacatgc tggtggacgg ccagggcaat ttcggctcgg tggacggcga
    11521 tgcgccggcc gcgatgcgct acaccgaagt gcgcatgtcg aagctggcgc acgagctgct
    11581 ggccgacatc gacaaggaca cggtggatgt cgtccccaac tatgacgagt cggagaccga
    11641 gccgtcggtg ctgcccaccc gtgtgccgaa cctgctggtc aacggctcct cgggcatcgc
    11701 ggtcggaatg gccaccaaca ttccgccgca caacctgacc gaaatctcca atgcgctgct
    11761 ggcactgatc gatgatcctt cgatctcgct ggccgggctg atgcagcacg tgccgggccc
    11821 ggattttccg accgccggca tcatcaacgg tgcgcaggaa atcgcggccg cgtacaaaac
    11881 cgggcgcggg cgcctctcga tccgcgcccg tgcgcacatc gaggacatgg acagtgccag
    11941 ccggcaggcg atcatcgtca ccgagcttcc ctatcaggtg aacaaggccc ggctgctcga
    12001 gcgcatggcc gaactggtgc gcaacaagca gatcgatggc atctccgagc tgcgcgatga
    12061 gtccgacaag gacggcatgc gggtcgtgat cgagctcaag cgcggcgaag tcgccgagat
    12121 cgtgctcaac aatctgtacg cacagacgcc gatggagacc gtgttcggca tcaacatggt
    12181 ggcgttgctc gacggtcagc ccaggctgat gtcgctgcgc gagatgctgg acgcgttcgt
    12241 gcggcatcgg cgcgaggtca tcacccgacg cacgatcttc gaactgcgca aggcgcgcga
    12301 acgcgcccac atactggaag gtctcgcggt tgcgctcgcc aacatcgacg aggtgatcgc
    12361 gctcatcaaa tcctccccga caccggccga ggcgcgcacc gcgatgctgg cgcgcgtgtt
    12421 ctcgccgggt gcggtggcgg ccatgctggg gacggccggc gccgcgcaga cccgtccgga
    12481 tggcctgtcg gtcgcgctcg gactcaccgc caccggctac cgcttgagcg aagagcaggc
    12541 gcaggcgatc ctggacatgc gcctgcaccg gctcaccggt ctggagcagg acaagatcgt
    12601 cgaggaatac cgcactctgc tggcgcagat cgcagacctg gcggacatcc tggcgcgccc
    12661 cgagcgcctg atgcaggtgg tgcgcgagga aatcgtccag atccgtgaca gctacggcga
    12721 tgcgcgccgc accgagatca gccgcgatca tctgaacctc agcaccgagg atctgatcga
    12781 gccgcaggac atggtggtca cgctgtcgca tgccgggtac gcaaaggcgc agccggtctc
    12841 cgactaccag gcgcagcgcc gcggcggtcg cggccgcgcc gccaccgcgg tcaaggacga
    12901 ggacttcatc gaaaagttct tcgtcgcgca cacgcatgac acgctgctgt gtttctccaa
    12961 tcgcggcaag atctactggc tgcgcgtgtt cgagctgcca cagggcggct tcggttcgcg
    13021 cggcaagccg gtggtgaatc tgctgccgct ggaagagggt gagaagatca gcgcggtgct
    13081 gccggtcaaa cagttcgacg agcagcactt cgtgttcatg gccaccagca gcggcacggt
    13141 caagaagacc ccgctggcgg cgttctcgcg gccgcggccg agcggcatca tcgcggtgga
    13201 tctgcgtggg gatgatcacc tggtgggtgt ctccctcacc gacggcaatc gcgagatcat
    13261 gctggtcacg agcggcggca agtcgattcg cttccacgag gacgaggtgc gtccgatggg
    13321 ccgtgaggcc gcgggcgtca tcggtgtgcg gctcggcacc ggtcagcggg tcatttcgct
    13381 gctggtgctg ggcgaagggc tgatcctgac cgcctcggag agcggctacg gcaaactcac
    13441 cccgctcgat gacttcccgt ctcacggtcg cggtgcgcag ggcgtcatcg cgctgcagac
    13501 caccgagcgc aacggtgcga ccgtggcggc actccaggtg caggcagggg atgagctgat
    13561 gctcatcagt tcgaccggca cgctggtgcg aaccccggtt gccgacattt cgatcgtcgg
    13621 ccgcaatacc cagggcgtac ggctgatccg gctgggtgac ggcgagcgcc ttaccggcat
    13681 cgagcgtgtg gccgccgggc tcgagaacgg cgatgacgtc gtcggcccgg gcagcgacga
    13741 cagctctacg ctgggcgaaa ccggaagcga cgaatccagt ccagtccaat agaatgaccg
    13801 gaccgcttcg attcacctga cagaaagggc tctcgcgtgc gcgtattcaa ttttgcagct
    13861 ggtcctgcgg ccttgcctct ggaggtcctc gaacgggccc gtgacgagat caccgactgg
    13921 aacggcagtg gcatgtcggt catggaagtc agccatcgcg gcaaggcctt catcgcagtg
    13981 gcggcgcagg cggaggcgga cctgcgcgag ctgctctcga tcccgaagca ctacaaagtg
    14041 ctgttcgtgc agggcggcgc caccggcatg ttctcggctg tgccgatgaa tctgacgcaa
    14101 gccgacagcg tcatcgatct ggtcaacacc ggctcgtggt ccaagaaggc cctgggtgaa
    14161 gccaggcgct attgcaaggt gtcggtggca gcggatgccg cggactgcaa ctacacgagc
    14221 gtgccgccgc agagtgcgtg gaagctgacg ccgaacgcgg cctatctgca ctactgcgcc
    14281 aatgagacca tcggcggcgt cgagttcgcg ttcattccgc aggtgccggt gccgctggtc
    14341 agcgacatgt cctccacgat cctgtcgcgt ccgctcgatg tgagccgctt tggcctcatc
    14401 tacgccggcg cgcagaagaa tatcgggccg gccggaatct gcgtcgtgat cgtgcgcgag
    14461 gatctgattg gcaaggcgcg ccccggcacg ccgtcggtgt gggacttcaa ggcgctggcc
    14521 gacgaaggct cgatgctcaa tacgcctcct acctttgcct ggtatctggc ggggctggtt
    14581 ttccagtggc tcaaggcaca gggcgggctg gcggcgatgg ccgagcgcaa tcgctccaaa
    14641 gcgcagctgc tctatcacac gatcgacagc aacccgttct atcgcaatcc ggtcgaaccc
    14701 tcgtcccgtt cgtggatgaa cgtgccgttc ctgctggcaa agccgcagct ggaagccagc
    14761 ttcctcagtg aagccaagca ggccggcctc atgaatctcg aaggacaccg ctcggtgggt
    14821 ggcatgcgcg ccagcctcta caacgcgacc acgctgcagg cggtgcaggc actgactgac
    14881 ttcatgcagc agttcgcccg ccgacacggt tgactgggga ggtggcgcag tgggattccg
    14941 gatactgacc ctgaacaaca tcagcatgcg cggactggaa cgcatgccgc gcgaaaccta
    15001 tgaagtctcg gcgcagatcg gcgagccgca cgcggtgatg gtgcgctcgg ccgacatgca
    15061 caagctcacg atcgcctcct cggtgctggc cgtggcgcgt gcgggcgccg gcgtcaacaa
    15121 cattccggtg gacgccctga gcaagcgcgg catcccggtg ttcaatgcgc ccggtgcgaa
    15181 tgccaacgcg gtgaaggaac tggtgctctc ggggctgttc ctcgcggcgc gtaacatctg
    15241 ccaggcatgg gtctatacgc gcgggctgag cggtgatgac cgcacgctcg atacgacggt
    15301 cgaggcgaac aaaaaaaact acgtcggctt cgagctgccg ggccgcacgc tcggggtgat
    15361 cggactgggc gcggtgggcg tgctggtggc caatgcggcc gaagccctcg gcatgaaagt
    15421 cttgggctac gatccgcaga tcaccgtcgc gagagcctgg cagctgaacg cctcggtgca
    15481 gcaggcgctg tcgctggatg atctgttcgc ccgttccgac atgatctcgg tgcatgtgcc
    15541 gctgctggat gcgacccgcg gtctggtgaa tgcagcgcgc ctggggctga tgcggcgggg
    15601 cggcaccgtg ctcaacttcg cgcgcgcccc gatcgtcaac gaacaggaca tggtggcggc
    15661 gctcaacgcc ggacagctgc actcctacat ctgtgacttc ccgacgcggg cgctcaagga
    15721 tcatccgcgc gcgatcacgt tgccgcatct gggcgcctcg acggtggaag ccgaagacaa
    15781 ctgcgcgatc atggcggccg atacgttgcg agaatttctc gagcaggggc acatccgcaa
    15841 ctgcgtgaat ttcccggaag cggtgctgcc gcgagaggtc ggctcgatcc gcctcgcgat
    15901 cgccaatgcc aatgtgccca acatggtcgg ccagatttcc acctgtctgg ccgacgccgg
    15961 cctcaacatc gccgaactgc tgaaccgctc gctgcgcgaa tatgcctata cgctcatcga
    16021 tgcggaaggg tcggccgatg aggcgctgtt gcagcggatc cgcgcgatcg atggagtgct
    16081 gtccgcgcgc ctggtgcgcg tcgcggactg aggcgcatgg cacgcgccag ggacagggcc
    16141 ggcagcagcg cgaagaggcg cgtgagcagc gcgaagagcc gcgcggcgac gcccagtgcc
    16201 gccccgccgg cggcggatct gcagacgctg cgcgcgcgca tcgattcggt cgaccagcgt
    16261 ctgcatgcgc tcatcaacga gcgcgcacgc ctggcgcagc aggccggcat ttccaagcac
    16321 aaggacggcc acacggtgga tttctaccgg cccgagcgcg aagccgaagt gctgcgcatg
    16381 gcgctggagc gcaatcacgg accgctgcgc aacgaagaga tcgtgcgcct gttccgcgag
    16441 atcatgtccg cctgtctggc acaggaggag ccgctgaaag tggcgttcct cgggcccgaa
    16501 ggcacgttca cgcagcaggc ggtgctcaag cacttcggcc actcggtgcg cgcactggcg
    16561 ctgccgacgg tggatgaggt gtttcaggag gtcgaaggcg ctgccgccga cttcggagtg
    16621 gtcgcgatcg agaattccag cgaggggacg atcaccaaca cgctcgaccg cttcctcgcc
    16681 agctcgctgc acatctgcgg tgaagtggaa ctgcgggtgc atcactgtct gatggggaag
    16741 atggattcgt tcgcgcgcgt gctgcgcgtg tgcgcccacg gtcaggcact ggcacagtcg
    16801 cgcggctggc tgaacgatca tctgcccgat gccgagcggg tgcccgtggc cagcaatgcg
    16861 gaaggcgcgc ggcgcgcccg cgatgagcag ggcaccgcgg cgatcgctgg tgaaatcgcg
    16921 gccgaggtct acggactgaa cgtcctggcc gccgaaattg aggatcgtcc cgacaactcg
    16981 acgcgctttc tggtgctcgg aagaaagatc ctgcgcacga gcggtgcgga tcgcacgacg
    17041 ctgctgctgt cggccggtga tacgcaaaaa cccggcgcgc tgcagcggct gctcgaaccg
    17101 ctggcgcgcc atcgcatcag catgagccgc atcgaatcgc gtccgtcccg ccgccgcaaa
    17161 tgggactatg tgttcttcat cgacatcgac ggacacgccg aggaccccaa cgtcaggaag
    17221 gccctcagcg cgctgaaaaa gcgggcctcg ctgttccgga ttctgggttc ctatccgcgg
    17281 gcggtgctgt gagcgcagcc ggtccgggcc ggctggcggt gccggcggtg cgcgggctgt
    17341 cgccttacat acccggcaag ccgatcgcgg agctggaacg cgaactcggc attcacgaca
    17401 tcatcaagct cgcgtccaat gagaacccgt atggtcccag tccgaaagtg cgcgcggcga
    17461 ttcagcgcgc catcgaggac gtgtggctct atcccgatgg cagctgctat gaactcaagc
    17521 aagcgctggc gcagcatctg aacgtcgatc cggtgtgcat cacggtcggc aatggctcga
    17581 atgatctgct gatgctgctg gcggaggcct ttctgaacgc cgatacgcat gcggtgcatt
    17641 cgcagtacgg ctttgcgatc tatccgctgg tgatccgcga g
//
LOCUS       DATMRM010000059         3470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1584981, whole genome shotgun sequence.
ACCESSION   DATMRM010000059 DATMRM010000000
VERSION     DATMRM010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584981"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VNZ06_01715"
     CDS             <1..361
                     /locus_tag="VNZ06_01715"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HWW19497.1"
                     /translation="AWVAAYIDGSFAPPAKSGGTRPDMTRGYFANADMADPIISPALH
                     PEVIAKFPPTLIITGTRAMDMSPAIYTNSQLLKAGVHSTLIVGEGMGHCYIYQSNLPE
                     ARDAYQVIVSFFRENLK"
     gene            complement(375..448)
                     /locus_tag="VNZ06_01720"
     tRNA            complement(375..448)
                     /locus_tag="VNZ06_01720"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(414..416),aa:Gly,seq:ccc)
     gene            complement(636..2864)
                     /locus_tag="VNZ06_01725"
     CDS             complement(636..2864)
                     /locus_tag="VNZ06_01725"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWW19498.1"
                     /translation="MYRSIIFRTVLPLLLAAGVVAYLGLPYIDRLLTSWFRSDVELRA
                     RLVASSVEETLPVLVAKHDQAGIRKYASKITADQRLVGLLICGADGWTEFKTERVPSV
                     VSCDSTARLADGQSDVMRAPWGSVLVSAYDEKPEGQAPYRVEIVNDLSFVDRRQDKAR
                     DFLITLACITLVIVALFLVLAAWLVIRRWANVLLRDISGRRFMDNAQSGPMSLPILRR
                     VHQVLREMEESQRLEIDYRENWTPQALQQVVREQLHSPQMIVVSNREPYSHEYGEHDS
                     IEIQVPASGMVTALEPVMRACSGTWVAHGSGTADREVVDRHDRLGVPPEEPSYTLRRV
                     WLSTSEEEGFYYGLSNEGLWPLCHLAYVRPAFRSEDWVAYQAVNRKFADAVLREAQSD
                     DPVILIQDYHFVLLPGMLRARRKSATIAMFWHIPWPNAETFGVCPWKQEMLSQLLMAD
                     ILGFHTLYHCQNFLGTVDRFIECQIDHAHMTVTLQGHVCHVAAYPISIEWPPRWLSAV
                     PDVPESRRRVREFFGIGEHVTLGLGVERWDFTKGVLERFLALELLLEQNPRMHGSLML
                     LQIAAPSRSKLPVYQSLQQATLSEVERINERFATPDWKPIVLIGKHQPPEQVFELYRA
                     ADFCIVNSLHDGMNLVAKEFVAARDDEDGVLILSTFAGASRELVEALLVNPFDVAETA
                     AAIRRAVEMPREQRRQRMQLMRRTVKENNVYRWAGRMLMDAARIRQRQALLTSEEELR
                     LT"
     gene            3006..>3470
                     /locus_tag="VNZ06_01730"
     CDS             3006..>3470
                     /locus_tag="VNZ06_01730"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HWW19499.1"
                     /translation="MAVLALTLALLFAVFRRKPASPRAVTTPSPPSRPSSAPAVPATQ
                     DEPAEELNARFEAMHRLHLLALSSDLLPTADDVLHEEVASKVRWTLSSITDKPNFAPR
                     RPLLLPKLVQAMNNDDVSRRELARIISSDPGLAGSLFRLANSPFYRISATPIE"
BASE COUNT          692 a         1092 c          992 g          694 t
ORIGIN      
        1 ggcatgggtc gcagcctata tcgacggctc cttcgcgcca ccggcgaaat ccggcggaac
       61 gcggcctgac atgacccgcg gctacttcgc caatgccgac atggctgacc cgatcatctc
      121 gcccgcgcta catccggagg tgatagcgaa gttcccgcct acgctcatca tcaccggcac
      181 gcgtgcgatg gacatgagcc cggcgatcta caccaattcg cagctgctga aggcaggcgt
      241 gcattccact ctcatcgtcg gcgaaggcat gggccattgc tatatctacc agtcgaacct
      301 gcctgaggcc cgggacgcat accaggtgat cgtcagtttc ttccgggaaa acctgaagta
      361 gcagaacggc ggtgtggagc gggtgatggg aatcgaaccc acgttagcag cttgggaagc
      421 tgcagttcta ccattgaact acacccgcgc ggatgcggga ttctattctg cgtgttatca
      481 ctcggacaac aggcgtgagg gcgatcagag gtcctcgtcg ccctcatcct cgcggacaac
      541 gggcaaagcg ggggactgaa cggcactccc gacgcccgcg caccgaatgc tgtcggccat
      601 cttggctgtg gggttgctgt gcggcgatcc tatccctacg tgagccgcaa ctcctcctcg
      661 ctcgtcagga gcgcctgtcg ctgtcggatt cgagctgcat ccatgagcat ccggccggcc
      721 cagcgataga cgttattttc ctttaccgtg cggcgcatca gctgcatacg ttgccgccgc
      781 tgttcgcgcg gcatctcgac cgcacgccgg atcgctgcgg cggtttcagc gacatcgaat
      841 ggattgacca acaaggcttc gaccagctcg cgcgatgcgc ctgcgaaggt gctcaatatc
      901 agcacgccgt cctcatcgtc gcgcgccgcg acgaattcct tcgcgaccag gttcatgcca
      961 tcgtgcaggc tattgacgat gcaaaagtca gcggcgcgat acaactcaaa tacctgctcg
     1021 ggtggctgat gcttgccgat cagtacaatc ggtttccaat caggcgtggc aaagcgctcg
     1081 ttgatgcgct cgacttccga tagcgtcgcc tgctgcagtg actgatagac cggcaatttg
     1141 ctgcgtgagg gcgccgcgat ctgcagcaac atcaaactgc cgtgcatgcg cgggttctgt
     1201 tcgagcagca gctccaaggc gagaaaacgt tccaatacgc ccttggtgaa gtcccagcgc
     1261 tcgactccca gccccagagt gacgtgctca ccgatgccga aaaactcgcg cacgcgacgc
     1321 cgcgattcag gaacgtcagg cacggcgctc agccagcgcg gcggccattc gatggagata
     1381 ggataggcgg cgacgtggca cacatgtcct tggagcgtga ccgtcatatg cgcgtgatca
     1441 atctggcact cgatgaaccg atccacggtc ccgaggaaat tctgacagtg atatagcgta
     1501 tggaaaccca ggatgtcggc catcagcagc tgcgagagca tctcctgctt ccacggacag
     1561 acaccaaagg tttcggcgtt gggccacggt atatgccaga acatcgcgat cgtcgcgctt
     1621 ttgcgccggg cccgcagcat tccgggtaac aatacgaagt gatagtcctg aataagaatc
     1681 accgggtcgt cagactgcgc ctcgcgcaac acggcatcgg caaacttgcg gttgaccgcc
     1741 tgataggcga cccagtcctc actgcggaat gcgggtctca cgtaggccag atggcatagg
     1801 ggccacagtc cctcattcga cagtccatag tagaagccct cttcttctga cgtggacagc
     1861 cacacgcgcc gcagcgtgta tgagggttcc tcgggaggca ccccgaggcg atcatgtcga
     1921 tcgaccacct cgcgatcggc ggttccgctc ccgtgcgcca cccacgttcc cgaacaggct
     1981 cgcatcaccg gttcgagcgc ggtcaccatc ccgctggccg gaacctgtat ttcgattgaa
     2041 tcgtgttcgc cgtattcgtg agaatagggc tcccggttgg ataccactat catctgcggc
     2101 gagtgcagct gttcgcgcac cacctgctgc agggcctgag gcgtccagtt ctcgcggtag
     2161 tcgatctcga ggcgctgact ttcttccatt tcgcgcagca cctgatgtac ccgacgcagt
     2221 atcggcagtg acatcggacc cgactgggcg ttgtccatga accggcgacc gctgatgtcg
     2281 cgtagcaaca cattggccca acgcctgatc accagccagg ccgccaatac tagaaataga
     2341 gccacgatta ccagcgtgat acaggccagc gtgatcaaaa aatcgcgcgc cttatcctgg
     2401 cgtcgatcga cgaaactcag atcgttgacg atttccacgc ggtagggggc ctggccttcg
     2461 ggcttctcgt cgtacgccga gaccagcact gaaccccaag gcgcgcgcat cacgtcgctt
     2521 tgcccgtcgg cgagccttgc cgtgctgtcg cagctgacca ccgagggtac gcgttcggtc
     2581 ttgaactcag tccaaccatc cgccccgcag atcagcagtc ccaccaaacg ctggtcggcg
     2641 gtgattttgc tggcgtactt cctgatgccg gcctggtcgt gtttggccac cagaaccggc
     2701 agcgtctcct cgaccgaact ggctaccagg cgggctcgaa gctctacgtc cgagcgaaac
     2761 cagctggtca gcagtcgatc gatatagggc aatcccaggt aggcaaccac tcccgcggcc
     2821 aacagcaggg gcaatacggt gcggaagatg atcgatcgat acatgtggtt tcaatatttg
     2881 tgccaacgac gctagccgcg cgcgatcgcg tccgtcaatg cacgaaaccc gcaatcgcgg
     2941 ctgaaatttt ttcaaattgc attaatctat gcgccgtgac cctattgcag attgtgtgtt
     3001 tgctgatggc tgttctggcg ctgacgttgg cgctgttgtt tgcggtcttc cgaagaaagc
     3061 ctgcaagccc gcgggctgtt accacgccgt caccgccgag ccgcccgtcc tccgctcccg
     3121 cagtacccgc cacgcaggat gagccggccg aagaattgaa cgctcgtttt gaggcgatgc
     3181 atcgactgca tctgctcgcg ctgagttccg acttgctgcc taccgccgac gatgtgctcc
     3241 atgaggaagt cgcttcgaag gttcgttgga cactatcgag catcacggac aaacccaact
     3301 tcgcgccgcg ccgcccgctg ctgcttccca aactggtgca ggcgatgaac aacgatgacg
     3361 tgtcacggcg cgaattggcg cgcatcattt cctcggatcc gggtcttgcc ggcagcttgt
     3421 tccgtttggc caatagcccc ttttaccgca taagtgctac gcccattgaa
//
LOCUS       DATMRM010000060         3797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2504237, whole genome shotgun sequence.
ACCESSION   DATMRM010000060 DATMRM010000000
VERSION     DATMRM010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2504237"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VNZ06_01735"
     CDS             <1..309
                     /locus_tag="VNZ06_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19500.1"
                     /translation="KNEGKSPEIVTKMIEGRLRKSLNEITLLGQPFVKDQDQTVEKLL
                     KAAKARVVRFERFEVGSGIEKKKEDFVAAVMAEVKRQTDPKESDPDPKKSDPKGPKSH
                     "
     gene            385..1113
                     /gene="pyrH"
                     /locus_tag="VNZ06_01740"
     CDS             385..1113
                     /gene="pyrH"
                     /locus_tag="VNZ06_01740"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWW19501.1"
                     /translation="MTDGKTPAWNRILVKLSGEALLGSADYGIDPAILKRIAAEIHDV
                     QALGTQVATVIGGGNLFRGAGLARSGMDRVTADQMGMLATVMNALALQDAIEQLGGQA
                     RVMSAIRINEVCEDFIRRRAVRHLEKGRTVIFAAGTGNPFFTTDTAASLRAIEIEAQL
                     LLKATKVNGIFSDDPIKNPQAKHYPRLTFDRVLDERLNVMDATAIVMCRDYRMPLRVF
                     NLGTPGDLLRVARGEDIGTLVTNE"
     gene            1127..1684
                     /gene="frr"
                     /locus_tag="VNZ06_01745"
     CDS             1127..1684
                     /gene="frr"
                     /locus_tag="VNZ06_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018679060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWW19502.1"
                     /translation="MLDDIKKEATQRMQKCVLVFQADLKKLRTGRAHPSLIEHLKVDY
                     YGSEVPLQQVSNISVEDARTLVVSPWEKSMVGPIEKAIHKSELGLTPNTAGTVIRIPL
                     PPLTEERRRDITKIVRQDAESARVGVRNVRRDSLADLKEMLKEKLLSQDDERRGQEEI
                     QKLTDRFVAEIDQQLAAKEKEILQV"
     gene            1681..2442
                     /gene="uppS"
                     /locus_tag="VNZ06_01750"
     CDS             1681..2442
                     /gene="uppS"
                     /locus_tag="VNZ06_01750"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HWW19503.1"
                     /translation="MSRAAANLASVPIPRHIAIIMDGNGRWARARGGIRSDGHRAGVE
                     PVRMAITECVRRHIGALTVFAFSSENWGRPRDEVLSIMSLFVESLEGEIAELHRNGVR
                     VRFIGDRSQLDQRMLASMATSERTTAQNQVLQLQIAVSYGGRADIVAAAQALVSQAER
                     GQLKAAEVDEVRFAQGLSLSDVPDPDLFIRTGGEHRISNFLLWNLAYTELYFTDRLWP
                     EFDVSEFEAALQFYAARQRRFGLTSEQLPAAEGER"
     gene            2452..3252
                     /locus_tag="VNZ06_01755"
     CDS             2452..3252
                     /locus_tag="VNZ06_01755"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HWW19504.1"
                     /translation="MLRLRILTAVALAVLLIAAILYFPASVTCVIFVGIVLIGAWEWS
                     AFLSLGAAGRALYVAVLAMLAVLGERLTLDPGYFLRSMELALCWWAFALIWIVCAPQR
                     GGRLAAGLAGMLTLLPTLIALVRIDSTWSNGAQWTLFILALAFAADTGAFFAGRQFGR
                     LPLAPRVSPNKTWEGVIGGMLLAAAVGGFGVIWFRLPALSFLSLCLAAAGCSVIGDLN
                     ESLLKRHVHLKDSGRLFPGHGGMLDRIDSVTAATPVMALGLIWLGVGK"
     gene            3317..>3797
                     /locus_tag="VNZ06_01760"
     CDS             3317..>3797
                     /locus_tag="VNZ06_01760"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF014700.4"
                     /note="catalyzes the formation of 2-C-methyl-D-erythritol
                     4-phosphate from 1-deoxy-D-xylulose-5-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HWW19505.1"
                     /translation="MTIGVAVLGSTGSVGVSTLEVLALHPERYRVTGLAARSNAAGLL
                     AQCQRFRPDWASLESPEAARELERGVRAAGLATRVAAGADALVELAADAKVGYVMAAI
                     SGAAGLRSTLSAAAAGKRLLLANKESAVMAGSLLLRAVESGGAELVPIDSEHNAIFQC
                     "
BASE COUNT          696 a         1088 c         1295 g          718 t
ORIGIN      
        1 aagaacgagg gaaagagtcc cgagatcgtg acgaagatga tcgagggccg gctgcgcaaa
       61 tcgctcaacg agatcacgct gctgggccag ccgttcgtga aggatcagga tcagacggtg
      121 gagaagctat tgaaggcggc caaggcgcgg gtcgtgcgct ttgagcgctt cgaagtcggc
      181 tcgggcatcg aaaagaagaa ggaggacttc gtcgccgccg tgatggcgga agtgaagcgg
      241 cagacggatc cgaaagaatc ggatccggat ccgaaaaaat cggacccgaa aggtccgaag
      301 tcgcattgac tgcaaacaga ccccgctaca gcggggtttt ctttatggca gactcaagat
      361 gcttccaaaa aggaacgtgc attcatgact gacggcaaga cgcccgcctg gaaccgcatt
      421 ctggtcaagc tgtccgggga agcgttgctg ggctcagccg actacggcat cgatcctgcc
      481 atactgaagc gaatcgccgc tgagattcac gatgtccagg ccttgggcac tcaggtcgcg
      541 accgtgatcg gcggcggcaa cctgtttcgt ggtgccggtc tggcgcgctc cggcatggac
      601 cgggtgactg ccgatcaaat gggaatgctc gcgaccgtca tgaacgcgct ggcgctgcag
      661 gacgccatcg agcagctcgg tgggcaggca cgggtcatgt ccgccatccg catcaacgag
      721 gtctgcgagg acttcattcg tcggcgcgcg gtgcgccatc tggagaaggg tcgcaccgtg
      781 atctttgctg ccggaaccgg caatccattc tttaccacgg atacggctgc cagcttgcgc
      841 gcgatcgaga tcgaggcaca gctgttgctc aaggcaacta aggtcaacgg tatattctcc
      901 gatgacccga tcaagaatcc gcaggcgaag cactacccgc gcctgacgtt tgaccgggtg
      961 ctggacgagc gcctcaatgt catggatgcg accgcgatcg tgatgtgccg cgactaccgc
     1021 atgccgctgc gagttttcaa tctggggacg ccgggggacc tgttgcgcgt cgcgcgcggc
     1081 gaggacatcg gcacactcgt taccaacgaa tgaaggcgtg actgccatgc tcgacgacat
     1141 caagaaggaa gcgactcaga gaatgcagaa gtgcgttctg gtgttccagg ccgacctgaa
     1201 gaaactgcgc accggccggg cgcacccgag tctgatcgaa cacctgaaag tggactacta
     1261 cggcagcgag gtgccgctgc agcaggtctc gaacatcagc gttgaggacg cccgcacgct
     1321 ggtggtctcg ccgtgggaaa agagcatggt tggcccgatc gagaaagcga tccacaagtc
     1381 ggaactcggt ctgacaccca ataccgccgg taccgtgatc cgcattccgc tgccgccact
     1441 gaccgaggaa cgccggcgtg atatcaccaa gatcgtgcgt caggacgcgg agagcgcccg
     1501 ggtaggcgtg cgcaacgtcc ggcgcgattc gctcgccgac ctgaaggaga tgctcaagga
     1561 aaaactcctg tcgcaggatg acgagcgcag gggacaggaa gagatacaga aactcactga
     1621 tcgtttcgtt gccgagatcg atcaacagct cgcggcgaag gaaaaggaaa ttcttcaggt
     1681 ttgagcaggg ctgccgcaaa cctcgcgtcc gtaccgatac cgcgtcacat cgccatcatc
     1741 atggatggca acgggcgctg ggctcgggcg cgcggaggaa tccgctcgga cgggcatcgc
     1801 gccggcgtcg agccggtgcg catggcaatc accgaatgcg tgcgtcgcca catcggggcg
     1861 ctgaccgtgt ttgccttcag cagcgagaac tggggacggc cgcgggacga ggtgttgagc
     1921 atcatgtcgc tgttcgttga gtcgctggaa ggcgaaattg cagagttgca tcgcaacggc
     1981 gtgcgcgtgc gcttcatcgg cgatcgcagt cagctcgacc agcgaatgct ggcgagtatg
     2041 gccacttccg agcgcaccac ggcacagaac caggttttgc agttgcagat cgcggtgagt
     2101 tacggtggtc gagcggacat cgtggccgcc gcgcaggctc tggtttcgca ggccgagcgc
     2161 gggcaactca aagccgcaga agtggacgag gtgcgctttg cccaggggct gtccttgtca
     2221 gacgtgcctg atccggatct gttcattcgt accggcggtg agcaccgcat cagcaacttt
     2281 ctgctgtgga acctggccta caccgagctg tatttcactg accggctgtg gccggaattc
     2341 gacgtttcgg aatttgaggc ggcgctgcag ttctacgcgg cccgtcagcg ccgtttcgga
     2401 ttgacctccg agcagctccc ggccgccgaa ggcgagcgtt agccgcgccg catgctgcgc
     2461 ttacgcatcc tgaccgccgt ggccctggcc gtactgctga tcgcagcgat tctgtatttc
     2521 ccggcttcgg tcacgtgcgt gatctttgtt ggcatcgttt tgatcggcgc ctgggagtgg
     2581 tcggccttcc tgagcctggg agccgcaggg cgtgcgctgt acgttgcggt actggcgatg
     2641 ctggctgtct tgggcgagcg gctgacgctc gatcccggct acttcctgcg cagcatggag
     2701 ttggcgctgt gctggtgggc attcgccctg atatggatcg tgtgcgcgcc acagcgcggt
     2761 ggccgtctgg cagcgggact ggccggcatg ctcacgctgc tgccgacatt gattgcgctg
     2821 gtgcgtatcg acagcacctg gagcaacggt gcgcagtgga cgctgttcat ccttgccctg
     2881 gcatttgccg cggataccgg ggcgttcttc gcaggacggc aatttggccg tctgccgctc
     2941 gctccgcggg tgtctcccaa caaaacttgg gagggcgtga ttggcggtat gctgttggcg
     3001 gcggccgttg gcgggttcgg tgtcatctgg tttcgtctgc cggcgttgag cttcctgagt
     3061 ctgtgtctgg ctgcggcggg ctgttcggtg atcggcgacc tgaacgagag cctgctgaag
     3121 cggcacgtgc atctgaaaga cagcggtcgg ctgtttccag gtcatggcgg catgctggat
     3181 cggatcgaca gcgtgaccgc ggccacgccg gtgatggccc tgggattgat ctggttggga
     3241 gttggcaaat gagaccgctc ggagccatgg ctttggggtt gatagggact gacagtcagg
     3301 cagcagggtg cggcggatga cgattggcgt tgcggtcctg ggatccaccg gcagcgtggg
     3361 cgtctccact ctggaggtgc tggcgctgca tcccgagcgc tatcgggtca cgggcctggc
     3421 ggcgcgcagc aacgcggccg gcctgctggc gcagtgtcag cgctttcggc ccgactgggc
     3481 ttcgctcgag tcgcccgaag cggcacgcga actggaacgt ggagtgcgtg cagccggtct
     3541 ggcgacccgg gtggctgccg gtgccgacgc actcgtcgag ctggctgccg acgccaaggt
     3601 cggctatgtg atggcggcga tcagtggcgc ggcggggctg cgttcgacct tgtcagcggc
     3661 ggctgccggc aagcggctat tacttgccaa caaggaatcg gcggtcatgg cgggctcatt
     3721 gctcctgcgc gctgtcgagt ccggaggagc agaattggtc ccgatcgaca gcgagcacaa
     3781 cgccatcttc cagtgca
//
LOCUS       DATMRM010000061        14423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2504553, whole genome shotgun sequence.
ACCESSION   DATMRM010000061 DATMRM010000000
VERSION     DATMRM010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14423
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2504553"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..351
                     /locus_tag="VNZ06_01765"
     CDS             127..351
                     /locus_tag="VNZ06_01765"
                     /inference="COORDINATES: protein motif:HMM:NF022832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HWW19506.1"
                     /translation="MSAEHLAQMANDIAHFFASETRHEDAIAGIANHIDKFWDPRMRR
                     QIEKHLAEGGEGLEPLAREAVARLTAAVTT"
     gene            348..3230
                     /locus_tag="VNZ06_01770"
     CDS             348..3230
                     /locus_tag="VNZ06_01770"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HWW19507.1"
                     /translation="MRRLPARAAATAALGLLLLGSVGHAADPVPPARMTPPAAATATK
                     PSLPTWAQTRSDIPADPAVRFGTLPNGMRYALMHNATPPNQVALRLRIGSGSLEEQAS
                     QQGLAHFLEHMAFRGSKSEADGEVKKSLERLGLQMGADTNAFTAQTETVYKFDLARND
                     DESIDHGLLLMRETGDALTLDPKLFDVERGVVESELRLTDVPARHMNDELLGFILPNQ
                     LASERLPIGKKPVLDAATVSLLRDYYRKWYRPERATLIVTGDIDVDSIEGKIRARFAD
                     WKASTAAARDPDLGKPTVRKPDARVFTEDGAPAQAQLLWVTPYDASVESVARDRHATL
                     RDLGFAVINRRLQAAAAAADRKFLSAGVGHRSPAYSAEIGALVVAYEADQWRPALLAA
                     EALRRQAVEGGIQQAELDREIDAYRSRLKAEVAGAATRTTPRLADQLVLCVDEDQVFS
                     SPQQDLAQFEAVVKDLSADTVNEVLRAEFMSGGPLAFVSNPNEIPDGDERALAALNDA
                     ERASVVAAAAVKKEAWPYTNFGTSGSVAERKRLEDLDLTEVRFANGVRLNIKPTKFRA
                     DQVLVSVHLSGGRASLPTDRPTLDWAAAALVLGGTGKLDFSAMTRVLAGKNYRVSFVV
                     NDSSDAFTGEATTADLTTQLQVLAAYVTDPAFRIEAFEQVRGLVQRQLTQVESSPQAV
                     FSLYLPSLLHSSDARWATPTAARVNAAQADELKGVLLPALAHGGIEITVVGDVTVDQV
                     IDSVAATFGAIAARPEGAAPAMEGVRFPAATATPVSVVHHGPAEQGVSAIAWPTADVF
                     ANTRQVAVRALLADVISARLFDSVRAADGAAYSPRAVASSSTVFKDYGYLLALADVPP
                     AKSQTFFDAMRKIEHDLTTDPVSDDELNRVRTPALAKLAQSQQSNGYWIGSLDRAQSD
                     PRFLDLERGRAASLKAVTAADIQEAAKSYLVDDKAFRLIVQSAGAP"
     gene            complement(3249..4274)
                     /locus_tag="VNZ06_01775"
     CDS             complement(3249..4274)
                     /locus_tag="VNZ06_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19508.1"
                     /translation="MSRIAVIGSTVDAAAPTAAQGGNPYGLTIAPATSGPIAQGDLIV
                     CNFNDSAGTEGAGTTLVGLHPSAGATPYNIAQSTSLQGCNAVAMLADDSIAAAAYSAN
                     TLPLVSSTGTLGSAFAAEPFAQPWGAAYVPATSAAPAALYVTNVGSGTGTASIDRIEL
                     NGDAAANFTEIAKGFCGSGKPGAIYAPAGLTYDPSIDTLYVVDTSSYSVLALSNVSKI
                     GSDGVLVNGGCGGATPTPALQFSGPSAAFARVIASGGQFNAPISAALLMDGDLIVGNG
                     DLDSPSTPNLAFEISASLGFVGKPLQLDGGMAGALFGIAATVDSAGNQIIYFNDDNTS
                     KVNVLTQ"
     gene            4360..4503
                     /locus_tag="VNZ06_01780"
     CDS             4360..4503
                     /locus_tag="VNZ06_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19509.1"
                     /translation="MKLPVAAGATVNEPFVGCEPLKFPPIPLDVLAVQDVASTERQDR
                     VSL"
     gene            4917..5495
                     /locus_tag="VNZ06_01785"
     CDS             4917..5495
                     /locus_tag="VNZ06_01785"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWW19510.1"
                     /translation="MKRLVVAGLLSTVIPLAALAALKQNDAAPAFTAQASLAGKTFQY
                     SLKDALKKGTVVVYFYPSAYTGGCNIQAHTFASNMDKFTAAGASVVGVSLDSIERLND
                     FSKDPDYCAGKLPVASDADGKIAKSYDLQVRDAVAGAKDTRGVEIGHGFAERTTFVVT
                     SDGKIAATIGGMRPDDNVDHALQVVQQLAAKK"
     gene            5571..5993
                     /locus_tag="VNZ06_01790"
     CDS             5571..5993
                     /locus_tag="VNZ06_01790"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HWW19511.1"
                     /translation="MNSKPSRLYAILASATLAIASASAMAADVKVKLVGAEETPPVTT
                     TATGSGTITVGADKSISGSVKTTGVEGVAAHIHSGAVGQAGPPIIPLTKGADGAWSVP
                     AGAKLSDEQYQAFKAGNLYVNVHSAEHKGGEIRGQLKP"
     gene            6023..6922
                     /locus_tag="VNZ06_01795"
     CDS             6023..6922
                     /locus_tag="VNZ06_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWW19512.1"
                     /translation="MTWRTWMAFLALCVIWGVPYFFIKLAVQDVSPFVVAWSRITLGA
                     LILLPYAWYRGALRAASGHWGAIAAFAFIEYVVPFSAISYGEQFIASSVTGILMAAVP
                     LSIAVLSRFFGVHEKLGTWRLLGLIMGFIGVAALVGFGTIQGTQGWIGVGCMLLATIG
                     YAIGPLIIMRHLSKLDSAGPAAGSLSIASLVLLVPALLSWPTHPPSGTAVASIAILGV
                     LCTALAMLFMFYLVSHAGASRASVITYVNPVVATLLGTLQLHERLGVAGVVAFALILL
                     GSWLATRGTPAQGESPPVTELAA"
     gene            6979..7827
                     /locus_tag="VNZ06_01800"
     CDS             6979..7827
                     /locus_tag="VNZ06_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWW19513.1"
                     /translation="MQQRFTGTERYIATDDLMMAVNAAIVLARPLLIKGEPGTGKTQL
                     AQEIARSLSRPLYEWHIKSTSKAQQGLYEYDAVSRLRDSQLGDERVQDIHNYIVKGRL
                     WEAFESEVQPVLLIDEIDKADIEFPNDLLRELDRMEFYVYETRELIKATHRPIIIITS
                     NNEKELPDAFLRRCFFHYIRFPDADTMRSIVDVHFPGLKQELLSEALRAFFEVRETPG
                     LKKKPSTSELLDWIKLLVAEDIDPAALRSTDQKKVIPILHGALLKNEQDVHLFEQLVF
                     LHRRAR"
     gene            7850..9094
                     /locus_tag="VNZ06_01805"
     CDS             7850..9094
                     /locus_tag="VNZ06_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWW19514.1"
                     /translation="MLVKFFFELRQGGVPVTLTEFLTLLEALRARVADVSAQDFYYLS
                     RLCLVKDERHFDRFDRVFAQVFQGAEKLFALLLPSIPSEWLRAATERNLTDEEKAKIE
                     ALGGWDKLLETLRQRLKEQKERHEGGKKWIGTAGTSPFGAQGFNPEGIRIGQSGGRNG
                     SAVKVWERREYRNFDDTIELGTRNIKLALRKLRKFAREGAPDEFDLDGTIRSTARNAG
                     LLDIKMIPERRNTVKVLLFLDVGGSMDYHVRVCEEMFSAARTEFKHLEHFYFHNFIYE
                     NVWKDNRRRFSVVTSTAQILRTYGQDYRVIFVGDATMSPYEILQPGGSIEHWNKEAGA
                     VWMQRICRTFPRLVWLNPENERAWEHSPSLQLTRDLVSARMFPLTLHGLDEAMRELRR
                     PLLRPQAPEIVAEGPPVEPPRI"
     gene            complement(9098..9508)
                     /locus_tag="VNZ06_01810"
     CDS             complement(9098..9508)
                     /locus_tag="VNZ06_01810"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HWW19515.1"
                     /translation="MPKITLVAITITILTLIQLMYFGALVGNARGKYQIKAPAVTGNE
                     MFERYYRVQLNSIEQIILFLPALWIANALAPVAYYWIALIGAVYLIGRMVYQISYVAE
                     PSKRSFGFALTSIPVLALLVIDLIGLVRGWIAPG"
     gene            complement(9624..10871)
                     /gene="frc"
                     /locus_tag="VNZ06_01815"
     CDS             complement(9624..10871)
                     /gene="frc"
                     /locus_tag="VNZ06_01815"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HWW19516.1"
                     /translation="MSKALEGVRILDFTHVQSGPTCTQLLAWFGADVIKVERAGAGDI
                     TREQLRDIPDVDSLYFTMLNHNKRSITLDTKNPRGKAVLERLIKHCDVLVENFAPGAL
                     DRMGFTWERIQQLNPKMIVASVKGFGPGPYEDCKVYENVAQCAGGAASTTGFDDGPPL
                     VTGAQIGDSGTGLHLALGIVCALYQRRSSGRGQKVLAAMQDGVLNLCRVKLRDQQRLA
                     RTGVMQEYPQYPHGQFLSSVPRSGNASGGGQPGWIVKCKGWESDPNAYIYVITQAPVW
                     KEICRVIGKDSWITDPNYATPPARLPRLTEVFTAIEDWTKTKTKFQAMEELNGHDIPC
                     GPILSMEELAAEPSLRASGTIVEVDHPKRGKYLSVGNPIKLSDSPTEVRRSPLLGEHT
                     EEVLSELGLSGAEIQSLRTENVI"
     gene            complement(10891..12282)
                     /gene="oxlT"
                     /locus_tag="VNZ06_01820"
     CDS             complement(10891..12282)
                     /gene="oxlT"
                     /locus_tag="VNZ06_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HWW19517.1"
                     /translation="MGTTSGGEFGADDDVDVDEPLARSSSTQPEAVVMGTRENSYGGS
                     VFANPWIQLILGIICMASVANLQYGWTWFVDPMSSKFHWTRAEIQVAFQCFVLIETWL
                     VPVEGYLVDRFGPRPVVIAGAVLVAIAWVINSMASSLFVLYVAAVIGGVGTGSVYGTC
                     IGNALKWFPRNRGLAAGITAAGFGAGAALTVIPIKNMIDTSGYQHAFLFFGLLQGTVV
                     LVMAMGLLVAAPSLIASAPKSHQAGRSFQPAQVLRSPCFWVMYLMFVLVAAGGLTLIP
                     NVASIAKDFGIAKTPVDLAGWVLPAAVFAVTLQRIFDGFGRPFFGWVSDHIGRENTMA
                     IAFVIGAIALYTLAQSGHNPILFVLVTAVYFGAFGEIYSLFPATQGDTFGSQFAAANS
                     GMLYTAKGAGSLLVASATALAVKGGWHQVFMIGVVFNLIAAALGMLVLKPMRARHLQR
                     TRTALDTVGAHTG"
     gene            12342..13043
                     /locus_tag="VNZ06_01825"
     CDS             12342..13043
                     /locus_tag="VNZ06_01825"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWW19518.1"
                     /translation="METNALLAGTLGLTPLNSASLRDQAYVLLKDAIAATDIYSQRQD
                     LRLDERQLTQALGVSRTPVREALTLLEQEGFIRTVPRRGIFIVRKCRREIIEMIQMWA
                     ALESMSARLATLHASDQEIGRLRHLFDEFKNSSPAEHIGEYSDANIAFHQMIIRLGGS
                     QLIADATRNLFIHVRAIRKATISQNDRATRSIVDHLRIIEALEQRNTELAERLTRQHT
                     LDLADHVERHCDFLD"
     gene            13074..14066
                     /locus_tag="VNZ06_01830"
     CDS             13074..14066
                     /locus_tag="VNZ06_01830"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HWW19519.1"
                     /translation="MKIAIVGAGAIGGFVGAKLALAGEQVTFMVRGANLAAIRANGIR
                     LVMSDGTEQVAGHVTASDDYHATGPQDMVILAVKAHQVEALVYDVPKLFGPDTVVVTM
                     QNGIPYWYFHRHGGELAGTVLRSVDPHGLIGQHITPGRVIGCVAYPASELTAPGVIHH
                     IEGDRFPVGELDGSTSARVTQVSESFIRAGFKSPILENIRSEIWLKLWGNLTFNPISA
                     LSRATLVDICQFPPTRLLAEMMMTEAQNIAHKLGITFRVPIERRIAGAEKVGKHKTSM
                     LQDVEAGREPELDALVGSVVELGRLTHTPTPTIDTVYGLSGLLAKVIAQSKADK"
     gene            14063..>14423
                     /locus_tag="VNZ06_01835"
     CDS             14063..>14423
                     /locus_tag="VNZ06_01835"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2993c-like domain-containing protein"
                     /protein_id="HWW19520.1"
                     /translation="MKHWARFRRHSNQQFGFGLLQGERITEYSGDLFGESRANGASVA
                     LSECALLSPCLPSKFIGLWNNFHALAAKIEKAEPAHPLYFIKASSALAGEGDIIRRPR
                     SYQGKIYYEGELGIVIGT"
BASE COUNT         2771 a         4466 c         4546 g         2640 t
ORIGIN      
        1 ctgtccggaa tacaaggtca ccgcagtgca ggtcacacgc gtgacgcagc cctcggcctg
       61 gcagcgccgg cacaaggagt tcgatgagca gcagcgccgg ctggctcacg tcgaggcgtc
      121 ctctgaatga gcgccgagca cctggcacaa atggcgaacg acatcgcgca tttcttcgcc
      181 tctgagacgc gtcacgaaga tgccatcgct ggcatcgcca accacatcga caaattctgg
      241 gatccacgta tgcgtcgaca gatcgaaaaa catctggctg aaggcgggga aggtctggag
      301 ccactggccc gggaagccgt ggcacgtctg acggctgcgg tcacgacgtg aggaggctgc
      361 cagcgcgcgc cgccgcgacc gcggcgctgg gcctgctgct gctcggcagt gtgggccatg
      421 ccgccgatcc ggtaccgcca gcccgaatga cgccgccggc ggccgccacc gcgaccaagc
      481 cttcgctacc cacgtgggcc cagacgcgca gcgacattcc ggctgatccg gcggtgcgat
      541 tcggcacgct gccgaatggg atgcgatacg ccctgatgca taacgccaca ccgcccaacc
      601 aggtggcgct gagactgcgt atcggctcgg gctcgctcga ggagcaggcc tcacagcagg
      661 gactggcgca tttcctggag cacatggcct tccgcggttc caaatccgaa gctgacggtg
      721 aggtcaagaa atccctcgag cgcctggggt tgcagatggg agccgatacc aacgccttta
      781 ccgctcagac cgagacggtc tacaaatttg acctcgcccg caacgacgat gaatcgatcg
      841 atcacggcct gctgctgatg cgcgagaccg gggatgcgct gacgctggat ccgaagctgt
      901 ttgacgtcga gcgcggcgtg gtcgaatccg aactgcgcct gacggatgtg ccggcgcgac
      961 acatgaacga tgaactgctg gggttcatcc tgcccaacca gctggcgagc gagcgtctgc
     1021 cgatcggcaa gaaaccggta ctggatgccg cgaccgtgag cttgctgcgc gactactacc
     1081 gcaagtggta ccgcccggag cgcgcgacac tcatcgtcac cggtgacatc gacgtcgaca
     1141 gcatcgaagg caagattcgt gcgcgcttcg ccgactggaa ggccagcact gccgctgcgc
     1201 gcgatccgga cttgggcaag ccgacggtgc gcaagcccga cgccagggta tttaccgaag
     1261 acggcgcgcc tgcacaggca cagttgctgt gggtcacacc ctacgacgca tccgtcgaat
     1321 cggttgccag ggatcgtcat gcgacgctgc gtgatctggg ctttgcggtc atcaatcgac
     1381 gtctgcaggc ggcggcagcg gcggccgatc gcaagttcct gtcggccggc gtcggtcatc
     1441 gcagtcccgc ctattcggcg gagatcggcg cgctggtggt ggcctatgag gcggaccagt
     1501 ggcggccggc gctactggcc gcagaagcgc tgcgtcgcca ggcggtggaa ggtggtattc
     1561 aacaggcgga gctcgatcgc gagatcgatg cctaccgctc gcggctgaaa gccgaagtcg
     1621 cgggtgcggc aacccgtacc accccgcggc tcgccgacca gctggttctg tgcgtggatg
     1681 aggatcaggt cttttcaagt ccacagcagg atctggctca gttcgaagcc gtcgtgaagg
     1741 atctgagcgc ggacaccgtc aatgaagtat tgcgcgcgga attcatgagt ggcggcccac
     1801 tggcatttgt gtccaatccc aatgagattc ccgacggcga cgaacgcgcg ctggcggcgc
     1861 tgaatgacgc cgagcgagcg agcgtcgttg ccgcagcggc cgtcaagaag gaagcctggc
     1921 cctacacaaa cttcggcacc agcggcagcg ttgccgaaag aaagaggctc gaggatctgg
     1981 atctgacgga agtgcgcttc gccaacggcg tacgcctcaa catcaagccc acgaagttcc
     2041 gggccgatca ggtcctggtg tcggtgcatc tgagcggcgg gcgcgcatcc ttgccgaccg
     2101 atcggccaac tctggactgg gctgctgcgg cgctggtact ggggggcacc ggtaaacttg
     2161 atttctcggc gatgacgcgg gttttggcgg gtaagaacta tcgggtcagc tttgtggtca
     2221 acgacagcag cgatgcgttt accggcgaag ccaccaccgc ggatctcacg acccaactgc
     2281 aggtgctggc agcctacgtc accgatcccg cgttccgcat cgaggccttc gagcaggtgc
     2341 gtggtctggt gcagcgccag ctcacccagg tcgagagcag cccgcaggct gtgttctcgc
     2401 tgtacctgcc gagcctgttg cattcctccg acgctcgttg ggcgaccccg accgcggcgc
     2461 gggtcaatgc ggcgcaggcg gatgagctca agggcgtact gttgccagcg ctggctcacg
     2521 gtggcattga gatcacggtc gtcggcgatg tgacggtcga tcaggtgatc gactctgtcg
     2581 cggccacttt cggcgcgatt gccgcgcgcc cggaaggtgc agcccccgcg atggagggtg
     2641 tgcgtttccc tgccgcgacg gccacgccag tgagtgtcgt gcaccacgga ccggccgagc
     2701 agggcgtcag cgcgatcgcc tggccaaccg ctgatgtctt tgcgaacacc cggcaggtgg
     2761 cggtgcgagc gctgctggcc gacgtcatct ctgcgaggct gtttgacagt gtgcgcgctg
     2821 ccgatggggc cgcgtattcg ccgcgcgcgg tcgcatcgag ttcgacggtc ttcaaggact
     2881 atggctatct gctggcactc gcagacgtgc cgcctgcgaa gtcgcagacg ttcttcgacg
     2941 cgatgcgcaa gatcgaacac gacctcacga ctgatcctgt gtcggacgac gagctgaatc
     3001 gcgtgcgcac gccggcgttg gccaagctcg cgcaatcgca gcagagcaac ggctactgga
     3061 tcggatcgct cgaccgcgcg cagtccgatc cgcgcttcct ggacctcgaa cgagggcgtg
     3121 ctgcgagcct gaaggccgtc accgcggccg acattcagga agcagccaag tcctatctgg
     3181 tggacgataa agcctttcgc ctgatcgtgc agtcggccgg cgctccatag cgccggccga
     3241 cgcgtcacct actgcgtgag cacattcacc ttgctggtat tgtcgtcatt gaagtagatg
     3301 atctgattgc cagcgctatc gaccgttgcg gcgatcccga acagcgcgcc ggccatcccg
     3361 ccatcgagct gcagcggctt gccgacgaag ccgagcgatg cggaaatctc aaacgcgaga
     3421 ttcggcgtgg acggactgtc aaggtcaccg ttgccgacga tcagatcgcc atccatgagc
     3481 agcgccgcgc tgatcggcgc attgaactgc ccacctgaag caatcacgcg tgcgaaggcc
     3541 gcagacgggc cgctgaactg cagcgccggc gtgggcgtcg cgccaccgca gccgccgttc
     3601 accagaacac cgtcgctgcc gatcttcgac acgttcgaca gggcgagcac gctgtaggag
     3661 ctggtatcca ccacatagag cgtgtcgatc gacggatcgt aggtcaggcc cgccggagca
     3721 tagatcgctc cgggcttacc ggacccgcag aagcccttcg cgatctcggt gaagttcgcc
     3781 gcagcatcgc cgttgagctc gatgcgatcg atgctggcag tgccagtccc gctgccgacg
     3841 ttggtcacat acagcgccgc cggcgctgcg ctggtcgcgg gcacgtacgc tgctccccag
     3901 ggctgcgcga acggttccgc ggcaaacgca ctgccaagcg tcccggtgct actgaccagc
     3961 ggcagcgtat tggcggaata ggccgccgcc gcaatactgt catccgccag catcgccacc
     4021 gcattgcagc cctgcaggct ggtggattga gcgatgttgt agggtgtggc cccggcactc
     4081 ggatgcagcc cgacgagtgt cgtgccggcg ccctcggtgc ccgcgctgtc gttgaagttg
     4141 cacacgatca gatcgccttg cgcgatcggc cccgacgttg ccggtgcaat cgtcagtcca
     4201 tacgggttgc cgccctgcgc ggcggttggc gctgcggcat cgaccgtcga gccgatgacc
     4261 gcgattctgc tcaattgact gaggatggag ggacctaccg tgacagtcac ggtgctcgcc
     4321 gttgccgcgc ccccggagcc ggcgcacgac aaggtgtaag tgaagctgcc ggtggcggcc
     4381 ggagctacgg tcaacgaacc gttcgtcggt tgtgaaccgc tgaaatttcc gcccattccg
     4441 ttggacgtgc tggcagtgca ggacgttgcg tcaaccgagc gccaggacag ggtcagcctc
     4501 tgacccaggc gaatgcccga tgcttgtgcc ggtgacgaga acgaaaccga tgcggccggt
     4561 ccgttgtaac cactgccgcc gcaactgctg agtgcggagg cgccgacgca agacagcaac
     4621 gtgctaacag tcgcacgtgt gtatctcatg gaactcctca taggtagcaa agaaacgcgt
     4681 ccttgagttc cgcaatcatt agaaaaagtt ttcgtgttcg atgaacacct cgttacattt
     4741 cattgcaaat ggtttgcgct cacaacggtt gcaccagtag cgcgcgacgt tgcgctggcc
     4801 gcaccaaacg cttgaccgcg gccgggcaga atgaggcaat acccgcacca aacaggtatc
     4861 attcatgagt agactagaat cgtttgctgt ctattccaca cacgaaggat ccacacatga
     4921 aacgcttagt cgtagccggt ctcttgtcga ccgtgattcc gctggccgcg ctcgcggctt
     4981 tgaagcagaa cgatgctgcg ccagctttta ccgcgcaggc ttcgctggca ggcaagacct
     5041 tccagtactc gctgaaggat gccctgaaga aaggaacggt ggtcgtgtac ttctatccgt
     5101 cggcgtacac cggtggatgc aatattcagg cgcacacgtt cgcaagcaac atggacaagt
     5161 tcaccgccgc cggcgcgagc gtcgtcggtg tgtcgctcga cagcattgag cggctcaatg
     5221 atttttccaa ggacccggac tattgcgccg gcaaacttcc ggtcgcctcc gatgcggatg
     5281 gcaagatcgc gaagtcctac gacctgcagg tcagggatgc ggtggcgggc gccaaggaca
     5341 cgcgcggggt ggagatcggt cacggcttcg cggagcgcac caccttcgtg gtgacctcgg
     5401 acggcaagat cgctgctact atcggcggca tgaggcctga cgacaatgtc gatcatgcac
     5461 tccaggttgt gcagcagctc gctgccaaga aatagtcgaa tcatttttcg acgagtcatt
     5521 ttcaacaatc aatcattttc aacaatcaac aaggggtagc aaccatgggt atgaacagca
     5581 agccatctcg gctctacgca atcctggctt cggcgacgct cgccatcgcc agcgcctcgg
     5641 caatggccgc ggacgtcaag gtcaagctgg tcggagcgga ggaaaccccg ccggtgacca
     5701 ccactgcgac cggcagcggc accatcaccg tcggcgccga caagtccatc agcggctcgg
     5761 tcaagacgac cggcgtggaa ggggtcgcgg cccacattca ctccggcgcg gtgggccagg
     5821 ccggcccgcc aatcattccg ctcactaaag gtgcggacgg agcctggagc gtgccggccg
     5881 gcgcgaaact ctcggatgag cagtatcagg ccttcaaggc cggtaatctg tatgtcaacg
     5941 tgcacagcgc ggaacacaaa ggtggcgaga tccgcggaca gctcaagccg tagagcgtga
     6001 cgtaccgaag gaatggacag cggtgacgtg gcgaacatgg atggcatttc tggcactctg
     6061 cgtcatctgg ggagtgcctt atttcttcat caagctggcc gtacaggatg tatcgccatt
     6121 cgtcgtggca tggagccgca tcaccctggg tgcgctgata ttgcttccct atgcctggta
     6181 ccgcggcgcc ttgcgcgccg cgtccgggca ctggggcgcg atcgcggcct ttgcattcat
     6241 tgagtacgtt gtaccgttct cggccatctc ctacggtgag cagttcatcg cctcatcggt
     6301 gaccggcatc ctgatggcgg cggtgccgct gtcgattgcc gtgctgtcga ggttcttcgg
     6361 agtgcacgaa aagctcggca cgtggcgtct gctcgggctg atcatgggtt tcatcggcgt
     6421 cgcggcgctg gtgggtttcg gcacgattca gggcacgcag ggctggatcg gggttggctg
     6481 catgctgctg gcaacgatcg gctatgccat cggcccgctg atcatcatgc gtcacctgag
     6541 caaactcgat tcggccggac cggcagccgg ctctctctcg atcgcaagcc tggtactgct
     6601 ggtaccggca ttgctgagct ggcccacgca cccgccatca ggcactgccg tggcctcgat
     6661 tgcgatcctc ggcgtgctgt gcaccgcgct ggccatgctg ttcatgttct atctggtgag
     6721 tcacgctggc gcctcgcgcg cctcggtgat cacctacgtc aatcccgtcg tggcgacgct
     6781 gctcggcacg ctccagctgc atgagcggct cggcgtggcc ggggtggtgg cctttgcact
     6841 gattctgctc ggctcatggc tggctacacg cggcacccct gcccagggcg agagcccgcc
     6901 ggtcactgaa ctcgcggcct gacataacgg cgcttccagg cgggtgtttt ccggtcaatc
     6961 agctacactg cttctactat gcaacaacgc ttcaccggta ccgaacgata cattgcgacc
     7021 gacgatctga tgatggcagt caatgctgcc atcgtcctgg cccgtccgct gctcatcaag
     7081 ggcgagcccg gaacgggcaa gacgcagctg gcacaggaaa ttgcacgctc gttgtcgcgg
     7141 ccgctgtacg agtggcacat caagtccacc agcaaggcgc aacagggtct gtacgaatac
     7201 gatgccgtgt cacgcttgcg cgactcgcag ctgggcgatg agcgcgtgca ggacatccac
     7261 aactacatcg tcaaaggccg tctgtgggaa gcctttgagt cggaagtgca gccggtgctg
     7321 ctgatcgatg agatcgataa ggccgacatc gagttcccga acgatctgct gcgcgaattg
     7381 gatcgcatgg agttctacgt ctacgagacg cgcgaactca tcaaggcgac ccaccggccg
     7441 atcatcatca ttaccagcaa caatgagaag gagctgccgg acgcgttcct gcgtcgctgc
     7501 ttcttccact acattcgctt tcccgacgcc gacaccatgc gctcgatcgt ggatgttcat
     7561 ttcccgggcc tgaaacagga gctgctgtcg gaggcgttgc gcgccttctt tgaagtgcgc
     7621 gagacgcccg gactgaagaa aaagccctcg acctcggagc tgcttgactg gatcaaactg
     7681 ctggtggcag aggatattga cccggcggcc ttgcgcagca ccgaccagaa gaaagtgatc
     7741 ccgatcctgc acggcgcact gctcaagaac gaacaggatg tgcatctgtt cgagcagctg
     7801 gtgttcctgc accggcgggc gcgttagcgc accttgaggg ctcagcgcga tgctggtgaa
     7861 gttcttcttc gagttgcgcc agggtggggt gcccgtcacg ctcaccgagt tcctgacact
     7921 gctcgaagcg ttgcgtgcgc gtgtcgcgga cgtctcggcg caggacttct actacctctc
     7981 gcggctgtgc ctggtgaagg acgagcgcca cttcgaccgc ttcgatcgcg tgttcgccca
     8041 agtgttccag ggtgccgaga agctgttcgc gctgctactg ccgtcgattc catccgagtg
     8101 gctcagggcc gcgaccgaac gcaacctcac cgatgaggaa aaggcgaaga tcgaggcact
     8161 gggcggctgg gacaagctgc tcgaaaccct caggcaacgc ctgaaggagc agaaggaacg
     8221 tcacgagggc ggcaagaagt ggatcggtac cgctggcacg tcgcccttcg gcgcgcaggg
     8281 tttcaatccg gaaggcatcc gcatcggtca gtccggcggt cgtaacggtt cggcggtcaa
     8341 ggtctgggag cggcgcgaat accgcaactt cgatgacacg atcgagctgg ggactcgcaa
     8401 catcaagctg gcgctgcgca agctgcgcaa gttcgcgcgc gagggcgccc cggatgaatt
     8461 cgatctggac ggcaccatcc gttccaccgc gcgcaacgcc ggactgctcg acatcaaaat
     8521 gattccggaa cggcgcaata ccgtcaaggt gctgctgttt ctggatgttg gcggctcgat
     8581 ggactatcac gtgcgcgtgt gcgaggagat gttttccgcg gcgcgtaccg agttcaagca
     8641 cctggagcac ttctacttcc acaatttcat ttacgagaac gtgtggaagg acaaccggcg
     8701 gcgcttctcg gtcgtgacct cgaccgccca gattctgcgc acttacgggc aggactatcg
     8761 tgtgattttc gtcggcgatg ccacgatgag tccgtacgag attctgcagc ccgggggcag
     8821 catcgagcac tggaacaagg aagccggcgc ggtctggatg caacgcatct gccgcacgtt
     8881 tccgcgcctg gtgtggctca acccggaaaa cgagcgcgcc tgggagcact caccgtcctt
     8941 gcagctcacg cgcgatctgg tcagcgcgcg aatgtttccg ctgacgctgc atggcctgga
     9001 tgaggccatg cgggagctgc gccgcccgtt gctgcgcccg caggcgcccg aaatcgtggc
     9061 ggaaggcccg ccggtcgagc cgccacggat ctgacggtca gcccggggcg atccagccgc
     9121 ggacgaggcc gatcaggtct atgaccaata gcgccagcac gggtatcgag gtgagggcga
     9181 atccgaagct gcgcttggac ggttcggcca cgtagctgat ctgataaacc atccgaccga
     9241 tcagatacac ggccccgatg agcgcgatcc aatagtaggc gaccggggcc agagcgttcg
     9301 cgatccacag cgccggcaga aacaggatga tctgctcgat gctgttgagc tgcacgcgat
     9361 agtagcgttc gaacatctca ttcccggtta cggccggtgc cttgatctgg tacttgccgc
     9421 gggcattgcc cacgagcgca ccgaaataca tcagttggat gagcgtcagt atcgtgatgg
     9481 tgatggccac gagcgtgatt ttgggcatgc gacaccgctt ggagttgttg gagactagtg
     9541 tagcgcttag ccaccaggtg tggcataggg ccggtgctgc agccgtctgc cgggcgaccg
     9601 gcggcaacgt agttgtgttg tcatcagatg acgttctctg tgcgcagtga ttgaatctcc
     9661 gcgccgctca ggccgagctc gctcagcacc tcttcggtgt gctcacccag cagcggtgag
     9721 cgccgcactt ccgtggggct gtcggatagt ttgatcggat tgcccaccga caaatacttg
     9781 ccgcgcttcg gatgatcgac ttcgacgatc gtaccgctgg cgcgcagtga cggttccgct
     9841 gccagctcct ccatcgacag gatgggtccg cagggaatgt catgcccgtt cagttcttcc
     9901 atcgcctgaa atttggtttt tgtcttggtc cagtcttcaa tcgcggtgaa gacttccgtc
     9961 agtcgcggca gtcgcgccgg cggcgtggcg tagttcggat ccgtgatcca gctatccttg
    10021 ccgatcacgc ggcagatctc cttccacacc ggtgcttgcg tgatcacgta gatgtaggca
    10081 ttgggatcgc tctcccagcc cttgcacttg acgatccagc cgggctgccc gccgccggaa
    10141 gcgttgcctg agcggggcac cgaggagagg aactgaccgt gcggatactg cgggtattcc
    10201 tgcatcacgc ccgtacgtgc gaggcgctgc tggtcgcgca gtttgacgcg gcacagattg
    10261 agcacaccat cctgcatcgc tgccaggact ttctgtccgc gaccgctgct cctgcgctga
    10321 tagagcgcgc acacgatgcc gagcgccaga tgcagacccg tgccgctgtc gccgatctgc
    10381 gcgccggtca ctagcggcgg accatcatcg aaccccgtgg tggaggcggc cccgccggcg
    10441 cactgcgcga cgttctcgta caccttgcag tcctcgtacg ggccgggacc gaaccctttc
    10501 acagaggcca cgatcatctt cggattgagc tgctgaatgc gctcccaggt aaagcccatg
    10561 cgatcgagcg cgccgggggc gaagttctcc accagcacat cgcagtgctt gatcagacgc
    10621 tcgagcaccg ccttgccgcg cggattcttg gtgtcgagcg tgatcgagcg cttgttgtgg
    10681 ttgagcatcg tgaagtacag gctgtccacg tccgggatgt cacgcagttg ctcgcgcgtg
    10741 atatctccgg cacccgcgcg ctccaccttg atgacgtccg cgccaaacca ggcaagcagc
    10801 tgtgtgcagg tcggtcctga ctgcacgtgc gtgaagtcca gtatgcgaac gccttccaag
    10861 gcctttgaca ttcaaactct cccggcgaac ttaaccagtg tgcgcaccca cggtatccaa
    10921 ggcggtgcgc gtgcgctgca gatgcctggc tcgcattggc ttgagcacga gcattcccag
    10981 ggctgcggcg atgaggttga agacgacgcc gatcatgaac acctggtgcc agccaccctt
    11041 gacagccagt gccgtcgccg aggccaccag caaggagccg gcgcctttcg cggtgtacag
    11101 catgccggaa ttggccgcgg caaactgcga gccgaaggta tcgccctgag tggcggggaa
    11161 caggctgtag atctcgccga acgcgccgaa gtaaaccgct gtgaccagca cgaacaggat
    11221 cgggttgtgg ccggactgcg ccagcgtata cagggcgatg gcaccaatga cgaacgcgat
    11281 ggccatcgtg ttctcacgac cgatgtggtc ggacacccag ccaaagaacg ggcgaccaaa
    11341 cccatcgaag atgcgttgca gcgtgaccgc gaagacggca gccggcagca cccagcctgc
    11401 gaggtctacc ggtgtcttgg caatcccgaa gtccttcgcg atcgaggcga catttggaat
    11461 caacgtcagc ccgcccgctg cgaccagcac gaacatgagg tacatcaccc agaaacaggg
    11521 gctgcgcagc acctgcgctg gctgaaaact gcgtcccgcc tgatgggact tgggtgccga
    11581 agcgatcagg ctgggagcag ccaccagcag tcccattgcc atgaccagca ccacggtgcc
    11641 ctgcaacagg ccgaagaaca ggaaggcgtg ctgatagcct gaagtgtcga tcatgttctt
    11701 gatcggaatg accgtcagcg ctgcgccggc cccgaacccg gcagccgtga tcccggcggc
    11761 cagaccgcgg ttgcgcggga accatttcag agcgttgccg atgcaggtac catacacgga
    11821 gccagtaccg acgccgccga tgacggccgc cacgtacagc acaaacagcg aactggccat
    11881 cgaattgatg acccacgcga tcgcgaccaa gacagcgccg gcgatcacca ccggcctcgg
    11941 gccaaaacgg tcgaccagat agccttcgac cgggaccagc caggtctcaa tgagtacgaa
    12001 acactggaac gccacctgaa tttcggctcg agtccagtga aacttggaac tcatcggatc
    12061 aacgaaccac gtccagccgt actgcagatt ggccacggag gccatgcaga tgattccgag
    12121 gatcagttga atccagggat tcgcaaacac cgagccgccg taactatttt ccctggtgcc
    12181 catgacgact gcttctggtt gagttgaaga ggaacgggcg agcggctcgt ccacgtcaac
    12241 gtcgtcatcg gcaccgaatt cgcccccgct cgtggtaccc atggtgcgtt cttgtgatat
    12301 acagtgtctg atatatcgta gcagaaccgc atggcctgat agtggaaacg aacgccctac
    12361 tcgccggcac actcgggttg acgccgctca attcagccag cctgcgcgat caggcctatg
    12421 tcctgctcaa ggacgcgatt gcggcgacgg acatttatag tcagcgtcag gacttacggc
    12481 tggatgagcg gcaattgacc caggctctgg gcgtcagtcg cacgccggtg cgcgaagcgc
    12541 tgaccctgct cgagcaggaa gggttcatcc gaacggtgcc gcgccgcggt atcttcatcg
    12601 tgcgcaagtg ccggcgcgag atcatcgaaa tgattcagat gtgggccgcg ctcgagagca
    12661 tgtcggcccg gctggccacg ctgcatgctt ccgatcagga gatcggtaga ctcaggcatc
    12721 tgttcgatga attcaagaac tcctcgccgg cggaacacat cggcgagtat tcggatgcga
    12781 acattgcctt ccaccagatg atcatccgtc tgggtggctc gcagctcatt gccgacgcga
    12841 ccaggaacct gttcattcac gtgcgcgcga ttcgcaaagc caccatctcg cagaatgatc
    12901 gtgccacccg ttcgatcgtc gatcatctca ggatcattga ggcgcttgaa cagcgcaaca
    12961 ccgagctggc ggagcggctc acgcgccaac acacactgga tctggcggat catgtcgagc
    13021 gtcactgcga tttcctcgac tgatctgcgc ccacatctgt tgggagaggc aggatgaaaa
    13081 tagcaatcgt cggcgcaggt gccatcggcg gcttcgtcgg tgccaagcta gccctggcgg
    13141 gcgagcaggt cactttcatg gtgcgcggtg cgaaccttgc ggccatccgc gccaacggta
    13201 tccgtctggt catgagcgac ggcacggaac aggtggccgg ccacgtcacc gcgagcgacg
    13261 actaccacgc gaccggaccg caggacatgg tgattctcgc ggtcaaggcg catcaggtcg
    13321 aagcgctggt gtacgacgtg cccaaactgt tcgggcctga caccgtggtc gtgacgatgc
    13381 agaacggcat cccctactgg tacttccatc gtcacggcgg cgagctggcc ggtaccgtgc
    13441 tacgcagcgt cgacccgcat ggcctgatcg gacaacacat caccccgggg cgcgtgatcg
    13501 gctgcgtggc ctacccggca tcggaactga ccgcaccggg ggtgattcat cacatcgaag
    13561 gggaccgctt tccggttgga gaactggatg gatcgaccag cgcccgggtc acgcaggtgt
    13621 ccgagtcgtt catccgcgcg ggcttcaaat cgccgatcct ggagaacatt cgctccgaga
    13681 tctggctgaa gctgtggggc aatctcacat tcaatccgat cagcgcactg tcgcgagcca
    13741 cgctggtgga tatctgccag ttcccgccca cgcgcctgct cgccgagatg atgatgaccg
    13801 aagcgcagaa tatcgctcac aagctcggca tcacgttccg cgtgccgatc gagcggcgca
    13861 tcgccggtgc cgagaaggtt ggcaagcaca agacctcgat gctgcaggac gtggaggccg
    13921 ggcgcgagcc ggaactggat gcgctggtcg gatcggtggt ggagctgggg cgactgaccc
    13981 atacacccac accgacgatt gacaccgttt acggattgtc aggcctgctg gcgaaggtca
    14041 tcgcccagag caaggccgac aagtgaagca ctgggcacga ttccggagac actcgaatca
    14101 acagttcgga ttcggcctgc tgcagggcga gcgcatcacc gaatatagcg gcgatctgtt
    14161 cggcgaaagc cgggcgaacg gtgcgagcgt ggcgctgtcg gaatgcgcgc tgctcagtcc
    14221 gtgcctgcct tcgaagttca ttgggctgtg gaacaacttt cacgcactcg cggccaagat
    14281 cgaaaaggcc gagccggcgc acccgctgta tttcatcaag gcgagcagcg ctctggccgg
    14341 tgagggcgac atcatccgtc gcccgcgcag ctatcagggc aagatctact acgaaggcga
    14401 gctcggcatc gtcatcggca cgc
//
LOCUS       DATMRM010000062         5972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1586239, whole genome shotgun sequence.
ACCESSION   DATMRM010000062 DATMRM010000000
VERSION     DATMRM010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586239"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VNZ06_01840"
     CDS             complement(<1..543)
                     /locus_tag="VNZ06_01840"
                     /inference="COORDINATES: protein motif:HMM:NF015748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HWW19521.1"
                     /translation="MNDVPIKCVEVFGFRVFSDDLDKIPLRQRCATVSTISPNSYGIA
                     TRDAEFRQALLEADFLVLDGVYFALGSILLDGRPIKPNQGPDVFFFFMRKLNAQSGRV
                     FFLGSSEDTLEKIRRRAALDYPQLSVGCYSPPFKPQFDDADNRLMLERINAFAPDVVF
                     VGMTAPKQEKWAHRHRDRINA"
     gene            complement(554..1294)
                     /locus_tag="VNZ06_01845"
     CDS             complement(554..1294)
                     /locus_tag="VNZ06_01845"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HWW19522.1"
                     /translation="MKRRLLRIANAAFKPFGVQLYKSGMDMDSVMRSMSARAVQVRTV
                     IDIGASDGRWSRRAMRFFPQARFIAVEPLREREARLKALRDSDPRFDYALCVAGEKDE
                     QIVELAVGADLDGSTVGGSEGAVRQVPSRSIDAIAALKQCVGPFLLKFDTHGFEVPIL
                     KGAARTLRDTHFIVMEVYNYRHTKGTLLFHEMCALLESSGFRCFNAADPMQRPLDGSL
                     WQMDLFFARHDNELFRENSYQRPRSPTA"
     gene            complement(1295..2296)
                     /locus_tag="VNZ06_01850"
     CDS             complement(1295..2296)
                     /locus_tag="VNZ06_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19523.1"
                     /translation="MEQLLRGKTVLLVGRDYFFYTREIALELTDSFGAVVHFLPIEPA
                     NLAYRILKKTGRLAPGWLQRYHRAAIARLRHASPDVVLFIQVHQIGDLVQEYRAAFAS
                     ARFVLYYWDSLRTHDYLPYVRYFDRTCTFDRADSRVHGLDYLPLFYAERFRSLRSSLA
                     ADCDVAFVGTAVSLGRYEQLEKFRAVARANGVSLIDYLVVSPFLYLRMLLHARRLRGV
                     HFRSLSEQRLLGFYGRARAILDLPNNLQSGLSMRTFEALGAHRKLITTNRDLMGEDLY
                     TADSVFVLGVDGEFPTRAFLVRPAQFADAVERYALRTWIARLLAPIAAGDGRAPGVA"
     gene            complement(2298..3506)
                     /locus_tag="VNZ06_01855"
     CDS             complement(2298..3506)
                     /locus_tag="VNZ06_01855"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWW19524.1"
                     /translation="MSLRKSDLNRRIILLLAVLMSGGSLLMPRLPVLVLIVVLCLAAR
                     GWLIDLKREMLPAVALLAIIFSVALFRTEAGEPESTAIRFANFVAGLLLLDVYLHADR
                     GALQRDLYAILKFMAWQSVLTVLLGETLSWLFIPIDVQDTYYYTLLGVFNFHYTLEES
                     TTLVRPDGFFYEPGVFQIYLNIFVYLALFVFRSTRWSILAVAALFATQSTTGVIICLI
                     LVGTYVTTRYINRGSLPARIAKFMFAVILIVPLVFVASGNIKQKLTGESQGSFLVRQY
                     DLLTGANVVLENPWLGIGFDYEQYHRAANRLGYADTSLPGQITLDRSNSNGVVFLLYS
                     IGIPLSIPFLIGMFRQVLFPQRILVGILLLLGLMSESIIFTPFFLMIIFSGLLLRRRF
                     APAARAVERA"
     gene            complement(3552..4151)
                     /locus_tag="VNZ06_01860"
     CDS             complement(3552..4151)
                     /locus_tag="VNZ06_01860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWW19525.1"
                     /translation="MMHFRIRDHLSVVLYRALFAPWFAGFGRRVRVVWPLRIVGCRYI
                     RLQDGVTLQYGAYLAAVGSNGVVPAIDVGAGTQIGNHAHIICTRRIDIGARVLIADRV
                     YISDNQHEYQDVRRAVLEQPVRQLADVVIGAGSWVGENACIIGCRIGRNCVIGANSVV
                     TRDLPDYCVAVGAPAIPVKRYCADRGTWRKTDSQGNFLD"
     gene            complement(4148..5434)
                     /locus_tag="VNZ06_01865"
     CDS             complement(4148..5434)
                     /locus_tag="VNZ06_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19526.1"
                     /translation="MFRSVRSLINERTSFVLVNVVVNMAFLLRSYVSMRVLGYQDLGL
                     AALMQTIILLISALQFGVINGGYRLLCSETGAAATQINNLVYTFVGALTLALFAVGGI
                     SAALFPGDGGVALVTLLGVCAGILTIVRNWMTNQLLATVMLRRLNLVNMLSALASLAP
                     LAVVRRAPLPICLASIVLQPLMFVVCLLVMEPGLRPSGWAVSAPLFRRILAAGFVVFL
                     TGIFLTINSQIERWSIVSCIGVAGLGHYYLALLFLNLYNLVPTSLGAVFLPRLVQTHM
                     QQDRAGMQQELRAFFRVTLYYSAAVMLCVWPLAAPVVALLLPKYLPDLRYVYLLTPGV
                     LIFGLSNPFAIVFNVLIQYRYYFYAYGLGTVATAGLVGLYILVTGTIDLDTLSIIKCA
                     VYTLMALIITAGYLVIARTHPEFRFELFGRKGTAAA"
     gene            complement(5451..>5972)
                     /locus_tag="VNZ06_01870"
     CDS             complement(5451..>5972)
                     /locus_tag="VNZ06_01870"
                     /inference="COORDINATES: protein motif:HMM:NF017011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19527.1"
                     /translation="NYMSEFYANQAATIENILRCLNGNQVLVVKEHPVDKGSLLRRKF
                     WDIRRRCSGLYYLPAEVHGREVLAVAQRVVTLTSTLGWEAAVLGKKVYVLGQIFYDRF
                     AAVTAISDFAALKRELGAPLTGTTLELERVSDFVASMAEQSHAGNPFPHPDLYDAHNR
                     LRVVEAITRALGL"
BASE COUNT         1233 a         1870 c         1909 g          960 t
ORIGIN      
        1 cgcgttgatc cggtcgcgat ggcggtgggc ccatttctcc tgcttgggcg ccgtcatgcc
       61 cacgaacacg acgtccggcg caaaggcgtt gatgcgctcc agcatgaggc ggttgtcggc
      121 gtcatcgaac tgcggcttga acggcggtga gtaacagccc acggacagct gcggatagtc
      181 cagcgccgca cggcggcgaa tcttctccag cgtgtcctcg gatgagccga gaaagaacac
      241 ccgtccgctc tgcgcattga gcttacgcat aaagaagaag aacacatccg gaccctggtt
      301 gggcttgatg gggcggccgt cgagcagaat gctgccgagc gcaaagtaca ccccgtccag
      361 cacgaggaaa tccgcctcca gcagcgcctg gcgaaactcc gcatcgcggg tggcgatgcc
      421 gtaggaattg gggctgatcg tgctcacggt ggcgcaccgc tggcgcaatg gaatcttgtc
      481 gagatcatcg gaaaacacgc gaaatccgaa gacttcgacg cacttgatgg gtacgtcgtt
      541 catcgtcacg ggctcaggcg gtcggcgaac gggggcgctg gtatgagttc tcacgaaaca
      601 gctcgttgtc gtgccgcgca aagaacaaat ccatctgcca caggctgcca tcgagcggcc
      661 gctgcatcgg gtccgccgca ttgaagcaac ggaagccgga tgactcgagc agcgcgcaca
      721 tctcgtgaaa cagcagcgtt cctttggtgt ggcggtagtt gtagacctcc atgacgatga
      781 agtgcgtgtc gcgcagggtc cttgcggcac ccttgaggat cggcacctcg aaaccgtggg
      841 tatcgaattt caggaggaac ggccccacgc actgcttcag cgcggcgatc gcatcgatgg
      901 aacgcgaggg cacttgtcgc accgctccct cgctgccgcc gaccgtgctg ccgtcgaggt
      961 cggcgcccac cgcaagctcg acgatctgtt cgtccttctc ccctgccacg cacagcgcgt
     1021 agtcgaagcg cggatcactg tcccgcagcg ccttgaggcg cgcctcgcgc tcgcgcagcg
     1081 gctctacagc gatgaacctc gcctgcggga agaacctcat ggcacgccgg ctccagcgtc
     1141 cgtcggaagc gccgatgtcg atgacggtgc gcacctgcac cgcgcgcgcc gacatgctgc
     1201 gcatgaccga gtccatgtcc atgccgcttt tgtatagctg cactccaaag ggcttgaagg
     1261 cggcgttagc gatccgcagc agtcgccgct tcattcaggc caccccgggc gcgcggccat
     1321 cgccggcagc aatcggagcc aggagcctgg cgatccaggt tcgcagcgca tagcgctcca
     1381 ccgcgtccgc gaactgcgcc ggacgcacga ggaacgcgcg cgtcgggaac tcaccgtcca
     1441 caccgaggac aaacaccgag tcggcggtat acagatcctc gcccatgagg tcgcgattcg
     1501 ttgtgatcag cttgcgatgc gccccgagtg cctcaaaggt ccgcattgag aggcccgact
     1561 gcaggttgtt tggaagatca aggatcgcgc gcgcacggcc gtagaacccc agcaggcgct
     1621 gttcagacag tgatctgaag tgcactcctc gcagccggcg cgcgtgcagc agcatccgca
     1681 ggtagagaaa cggcgaaacc acgagatagt ctatcaggga cacaccgttg gctcgcgcca
     1741 cggcacggaa cttctcgagt tgttcgtagc gcccgaggct taccgccgtc ccaacaaacg
     1801 ctacatcgca atctgccgcc agcgaggacc gcagggagcg gaagcgctcg gcgtagaaca
     1861 gcggcaggta gtcaagaccg tgaacgcggc tgtcggcccg gtcgaaggta caggtgcggt
     1921 cgaagtagcg aacgtaaggc agatagtcat gggttcgcag cgagtcccag tagtacagca
     1981 cgaagcgcgc cgacgcgaat gcggcgcgat actcctgcac caggtccccg atctggtgca
     2041 cctggatgaa cagcaccacg tccggggacg cgtgccgcag gcgcgcgatc gcggcgcggt
     2101 ggtagcgctg gagccagcct ggcgcgaggc gacccgtttt cttcaggatc cggtaggcga
     2161 gattcgccgg ctcgatgggc aggaaatgca ccacggcgcc gaagctgtcc gtcagttcca
     2221 gcgctatttc gcgggtatag aaaaagtaat cacgccccac cagcaggacc gtcttgccgc
     2281 gcagcaactg ctccatctca ggcgcgctcc acggcccgcg ctgccggggc gaagcgccgg
     2341 cgtagcagca ggccgctgaa gatgatcatc agaaaaaatg gcgtgaagat tatggattcg
     2401 ctcatcaggc ccagcagcag cagaatcccc accaggatgc gctgcggaaa caacacctgg
     2461 cgaaacatgc cgatcagaaa cggaatgctc agaggaatgc cgatgctgta gagcaggaag
     2521 acgacgccgt tgctgttgct gcggtccagg gtaatctggc ccggcagcga ggtgtccgcg
     2581 taccccaggc ggttggccgc gcgatggtac tgctcgtagt cgaagccaat gcccagccag
     2641 ggattctcga ggacgacatt cgcccccgtg agcagatcgt actgacgtac gaggaatgaa
     2701 ccctgagatt ctccggtgag cttttgcttg atgttgccgg atgccacgaa caccagcggc
     2761 acgatcagaa taactgcaaa catgaacttg gcgatccgcg ccggcaggct gccccgattg
     2821 atgtaacgcg tggtcacgta cgtgcctacc aggatcaggc agatgatgac tccggtggtc
     2881 gactgggtcg cgaacaaggc ggccaccgcc aggatcgacc agcgggtgct tctgaacacg
     2941 aacagcgcca gatacacgaa tatgttcagg tagatctgga acactcccgg ttcgtaaaag
     3001 aagccgtcgg gtcggaccag tgtcgtgctc tcctcgaggg tgtagtggaa attgaacacc
     3061 ccgagaagtg tgtagtagta ggtgtcctgc acgtcgattg ggataaacag ccagctcagg
     3121 gtctccccca gcagcaccgt gagtacggac tgccacgcca tgaacttcag gatcgcgtac
     3181 aggtcgcgct gcagcgcgcc gcggtcggcg tgcaggtaga catcgagcag cagcagcccg
     3241 gcgacgaagt tggcgaagcg gatcgcggtt gattccggct cgccggcttc ggtgcgaaac
     3301 aacgcgacgc tgaagatgat cgccagcagc gctactgcgg gcagcatctc gcgcttcagg
     3361 tcgatcagcc agccgcgtgc ggcaagacac agcaccacga tcagcaccag gaccggtagc
     3421 cgcggcatca gcagcgagcc accgctcatc agcaccgcga gcagcaggat gatgcggcgg
     3481 ttgaggtcgg acttgcgcag gctcacagcg attcccctgg ccgcagcagg cgcgctggcg
     3541 ccgaccgccg ctcagtccag aaagttgcct tgggaatcgg ttttcctcca cgtgccacgg
     3601 tccgcacagt atcgcttcac cggtattgcg ggcgcgccca ccgccacgca atagtcgggc
     3661 aggtcgcggg tcaccacgct gttggccccg atcacgcaat tccggccgat ccggcagccg
     3721 atgatgcagg cgttttcgcc cacccaggag ccggcgccga ttaccacatc cgcaagttgc
     3781 cgcaccggct gctcgaggac cgcgcgccgc acgtcctggt actcgtgctg attgtccgaa
     3841 atgtagacgc ggtcggcaat cagcacgcgc gcgccgatgt cgatgcgccg ggtgcagatt
     3901 atgtgggcgt ggttgccgat ctgggtgccc gcgcccacgt cgatcgcggg cacgacgccg
     3961 ttgctgccga cggccgcaag atatgcgccg tactgcaagg tgacaccgtc ctgcagacgg
     4021 atgtagcggc agccgacgat gcgcagcggc cagaccacgc gcacgcggcg gccgaagcct
     4081 gcgaaccacg gcgcgaacag cgcccggtag agcactaccg acagatgatc gcggattctg
     4141 aaatgcatca tgccgctgcg gttcccttgc ggccgaacag ttcgaagcga aactccgggt
     4201 gcgtgcgcgc gatgaccagg tacccggcgg tgatgatcag ggccatcagg gtgtagacgg
     4261 cgcatttgat gatcgacagc gtgtcaagat cgatcgtgcc ggtgactaga atataaagcc
     4321 ccacgagtcc cgcggtggcc accgtgccca gaccgtaggc atagaagtag tagcggtact
     4381 ggataaggac gttgaagacg atcgcgaatg gattgctcag tccgaaaatc agcaccccgg
     4441 gtgtcagcag gtacacgtag cgcaggtcag ggagatattt cggcagcagc agcgcgacta
     4501 ccggcgctgc cagcggccac acgcacagca tgacggcggc ggaataatag agtgtgacgc
     4561 ggaagaaggc tcgcaactcc tgctgcatgc cggcccggtc ctgctgcatg tgcgtctgga
     4621 ccagcctcgg cagaaacacc gcccccaggg atgttggcac gagattgtag agattcagga
     4681 acagcagcgc gaggtagtag tgtcccagcc ccgccacgcc gatgcaggag acgattgacc
     4741 agcgctcgat ctggctgttg atggtcagga atatccccgt cagaaacacc acgaaccccg
     4801 cggccaggat cctgcggaac agcggcgccg agaccgccca gccgctcggc cgcaggccgg
     4861 gctccatcac gagcagacag acgacgaaca tcaggggctg cagcacgatc gatgcaagac
     4921 agatcggcag cggcgcgcgc ctcacgaccg ccagcggcgc gagcgaggcc agggcggata
     4981 gcatgttcac caggttcagc cgccgcagca tgacggtggc aagcaactgg ttggtcatcc
     5041 agtttctgac gatggtgagg atcccggcgc agacgccgag aagcgtcacc agcgccacgc
     5101 cgccatcacc cggaaacagc gccgcgctga tgccgccgac cgcaaacaag gcgagcgtga
     5161 gcgcgcccac gaacgtgtag accaggttgt tgatctgggt ggccgccgca cccgtttcag
     5221 agcacagcag tcgatagccg ccgttgatga caccgaactg cagcgcgctg atcagcagga
     5281 ttatcgtctg catgagcgcc gccaggccca gatcctggta gccgagtacg cgcatcgaca
     5341 cgtagctgcg cagcaggaac gccatgttga cgacgacgtt gaccagcacg aatgacgtgc
     5401 gctcattgat cagggaacgg accgagcgga acattggcgc agtgaccgct ctacaatccg
     5461 agcgcgcgcg tgatcgcctc gactacccgc agccgattgt gcgcgtcata caggtcggga
     5521 tggggaaagg gattccccgc gtgtgactgt tcggccatgc tggcaacaaa gtcgctcacg
     5581 cgctccagct cgagcgtcgt gccggtcagc ggcgcaccca gctcgcgttt gagcgccgcg
     5641 aaatcgctga tcgcggtaac tgcggcgaaa cggtcgtaaa agatctgccc gagcacatac
     5701 accttcttgc cgagcactgc ggcctcccat ccgagcgtgc tggtcagggt gacgacgcgc
     5761 tgcgcgacgg ccaggacctc gcgcccgtgg acctcggcgg gcagatagta caggcccgag
     5821 cagcggcgcc tgatgtccca gaacttgcgc cgcagcagcg agcccttgtc gaccggatgc
     5881 tccttgacca ccaggacctg gttgccgttc aggcatctga ggatgttctc gatggtggcg
     5941 gcctggttag catagaactc cgacatgtag tt
//
LOCUS       DATMRM010000063        15468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_26971, whole genome shotgun sequence.
ACCESSION   DATMRM010000063 DATMRM010000000
VERSION     DATMRM010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15468
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26971"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VNZ06_01875"
     CDS             complement(<1..521)
                     /locus_tag="VNZ06_01875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008080626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HWW19528.1"
                     /translation="MSEGRSGNTDFGFQQVPWKEKATRVRAVFDSVSGRYDLMNDLMS
                     GGAHRLWKHFTLRLTGLSDGQQALDLAGGTGDLAAGMARQVGERGRVVLSDINRSMLA
                     RGRDRLTDRGLIANIQYVQADAERLPFASSQFDCVTIGFGLRNVTDKTAALASIHRVL
                     KPGGQLLVLEFSQP"
     gene            complement(862..1215)
                     /locus_tag="VNZ06_01880"
     CDS             complement(862..1215)
                     /locus_tag="VNZ06_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019581848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HWW19529.1"
                     /translation="MNTVQATEIKLRQRSRVLEVSFDDGSRFELPFEFLRVHSPSAEV
                     QGHSPDQRVLVLDKQQVGIRAVEPIGQYAVKLSFDDGHDTGLYTWKYLYQLGCQREQL
                     WSEYLARVEHSGHRR"
     gene            1281..3089
                     /locus_tag="VNZ06_01885"
     CDS             1281..3089
                     /locus_tag="VNZ06_01885"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWW19530.1"
                     /translation="MKSTDTSNPDYFHRVVDCQWACPAHTDVPEYIRLIAQGRYTDAY
                     MLNRESNVFPGILGRVCDRPCEPACRRSRVEDKPVAICRLKRVAADLKGELGGLLPKA
                     PAIKNGKRIACIGAGPASLTIGNDLAPLGYEVTIFEQNAVPGGLMRSNIPSFRLPQSV
                     LDEEIGMILDMGVHLKLSSPIESLKQLLDEGGFDAVFVGSGAPKGKELKLPGRDQTDR
                     IHIGITWLESVAFGHIGSIGERVLIIGVGNTAMDCCRTSLRLGAHSVKVMARKPRGFF
                     KASEWELEDAEEENVEIIVNHSPKSFVIEHGKLTGMLFDKMEYDLENAVIKAERLVGE
                     VMIPCDDVILAIGQENAFTWVERDIGVKFNKWDQPVVDETTFQSTHSKVFFGGDAAQG
                     PKNIIWAVEQAHQAAISIHNYCCGVAVTERPASRMQLSSRKMGMHEWAYKNDYAALER
                     RLMPHVSLKERFKKLNIEVELGFSVEQAAQEAQRCLNCDVQTVFDAKLCIECDACIDI
                     CPVDCLTITRGGEEADLKTRLRAPVLEPTQLLYVSDALPQTGRVMVKDENLCVHCGLC
                     AERCPTAAWDMQKSTIHWPHADEGGPKCQPQKLKTA"
     gene            3059..4894
                     /locus_tag="VNZ06_01890"
     CDS             3059..4894
                     /locus_tag="VNZ06_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HWW19531.1"
                     /translation="MPTAKAQDRVNDFAVKIATVNGTGSASANGLLMKAIFRSGVPVV
                     GKNYFPSNIQGLPTWYEIRITRDAHRARSGAVDIMVALNAETYARDLAEVASGGYFIY
                     DSTWPRPALLKRDDITVIGVPLAQLCNDAFQGVRTRILMKNICYAGVLAALCQLDLDV
                     IRDLLGETYAKKPQLVDANMKAIQLGYDYARANFACPLGLRVERMNATAGHIIIDGNT
                     AAALGCVYAGATVGAWYPITPSTSLMDAYKSFCDRLRVDPVTKRKNYIMIQAEDELAS
                     IGMVIGGCWNGARAFTATSGPGISLMQEFLGLAYYAEVPAVVFDIQRVGPSTGMPTRT
                     QQCDIHSAAYASHGDTRQVCLYPASPEECFEFAPKAFDLAERLQTPVLVLSDLDIGMN
                     DWMCPEFKWDESYVPDRGKVLDVEQVKQLEKFWRYYDHDNDGIAQRTLPGTHPNGAYF
                     ARGSGHNQYGGYTEDSKEYQIVVDRLRKKWITASHLVPKAIIERNEKIDIGIVSLGSS
                     DGAVREARARLEAQGIHTNYMRIRGFPFGEEVEAFLQAQRLSFVVEQNRDAQLRSLLT
                     LETGIEKTRLRSILHYNGMPIPASCVYDGVAAVITPNRPAAQLVS"
     gene            4915..5970
                     /locus_tag="VNZ06_01895"
     CDS             4915..5970
                     /locus_tag="VNZ06_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HWW19532.1"
                     /translation="MTFMAKPKIVQLELPKNALGLTRRDYEGANSTLCAGCGHDSITA
                     AIVEACWESALRPENIVKLSGIGCSSKTTAYFVRGGHGFNSVHGRMPSIATGANAANR
                     ELTYIGVSGDGDTLSIGLGQFCHAIRRNLNMVYVIENNGVYGLTKGQFSASADVGSTA
                     KKGEVNNQPPIDPVQLAITLGVSFVARSFSGDKAQLVPLLQAAMHHQGFSLLDVISPC
                     VTFNDHEGSTRSYAYTREHYDEVVHADFVPPAQEIAADYAPGETHAVTMHDGSRVLLR
                     KVEQDYDPTDRGVALDMIQRRLKQGEYLTGLLYIGRDQAELHQLNATPDLPLNRLPYA
                     QLNPGPQALAKTLARYR"
     gene            5984..7339
                     /locus_tag="VNZ06_01900"
     CDS             5984..7339
                     /locus_tag="VNZ06_01900"
                     /inference="COORDINATES: protein motif:HMM:NF017835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HWW19533.1"
                     /translation="MKWAIIAAASAVMVALLGVAAYLVHTRRPSILEVAAFGHLRTYP
                     AWWRPKGFVYLFSGERGWSAVDEQTARDLARSGNLVFGVDAPEFLQVQNRLDGCIYLP
                     GILEDYSRPWQHSADMAQYLAPAVLGSDIGATLAYEAQVQAPPTAFSAVVAVDPVSAV
                     SMRRPFCEHPAAAMTSSEQTIKSEPPGRNVPLRIVLDSSASKAARQFASSIPGQTPTT
                     VKTAQPRYRSYRQALEDIHTQMQKSGIADLPLAIVPASQSGCANGFAVLYSGDGGWRD
                     LDRSLADILASKGMSVIGVDVLRYYWKDKSPDVAARDLTRMIRYYGEQWHCHKVALIG
                     FSFGADILPFVASRTPPDVRSELVLMTLLSPERSTAFEIEMNGWLGGKPKAGVPIGPE
                     VRKLDGLKVQCVYGRDESADSLCTDSSAGPLTVLAKSGGHHFDQDYNELADDILAALP
                     K"
     gene            7389..8135
                     /locus_tag="VNZ06_01905"
     CDS             7389..8135
                     /locus_tag="VNZ06_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWW19534.1"
                     /translation="MTHAATQSTAWHFAQVHGHRIYYALRGDGPTLVLLHGGGDSGEH
                     SFEHQLDVFAEHHHIVAPDQVGQGRTPAAPGPLSYTGMMEDTAALLTEMKLRHVDLVG
                     FSDGGILALMLALRHPELVRRVVISGVNVSPEGLNSESREGLRAVQADDPKTIDEKLA
                     RLWLTSPAESELSLQLLKNVGQPVLVVSGDRDAITLEHTLQIFHALPRAELCILPGTD
                     HGTFSGRPEWLNPIITHFLNREADPGPTPK"
     gene            8266..8781
                     /locus_tag="VNZ06_01910"
     CDS             8266..8781
                     /locus_tag="VNZ06_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWW19535.1"
                     /translation="MNPPYLLAQGSGTTRLPLRFHSTLPRLKLADSATRAMIDFQRDP
                     PLTVDESYSLEEAIDRMFRLGVRAFLVVRDLSVVGLMTASEATRSTPQHSRVADAMTP
                     TDDVPAIDWHTLAESRVRDLVEIFDATGVNYLVVLESHSASLSSVRGLIQHKRLQRQL
                     SSPWSLRAAGI"
     gene            8798..9871
                     /locus_tag="VNZ06_01915"
     CDS             8798..9871
                     /locus_tag="VNZ06_01915"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWW19536.1"
                     /translation="MSRCSKGAREVLQFRRVRRHLLMAAGGLWVHGALAAAVPLALGS
                     ARGALVDVHHHWSNTALLQWWNRSAATAGWAADKALAAMDAAGVRTAMLSVTQPGVWN
                     SSDGDGSVRIARTCNEAMARITQDHLGRFGYFAAVALPMVPASVAEIGYALDALKADG
                     IGLLSSYDGSYLGDPSFTPVFEELNARRAVVYVHPAVPLCCANLVPEIPASALEAPSD
                     TSRTIESLLVSGALARFSAIRFIFAHGGGTLPFLAERLLDSLVPDPPADKSYLSRYNA
                     KVALAQLYFDSASVANAPAWAALTSFTTPDRILFGSDYPAHEVRAQLGSLRAMEQRFG
                     VALADARSIESGNAQHLFAARLS"
     gene            9888..10787
                     /locus_tag="VNZ06_01920"
     CDS             9888..10787
                     /locus_tag="VNZ06_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HWW19537.1"
                     /translation="MPELPDVTVYLEALSERIIAQRLDRIALINPFLLRTATPPLKSA
                     ESRRVERLRRVGKRIAIGLTDELWLVLHLMIAGRLHWFGRGSKVSGRAALARFEFESG
                     TLTLTEAGSKRRASLHLISGESNLARMDPGGLEVLEADLDAFTARLRLENHTLKRALT
                     DPHLFSGIGNAYSDEILHRARLSPLALTSRLSADEIARLYEATRGSLTDWTELLRQEA
                     GGKFPEGVTALRPQMAVHGRYGQACPICGTTVQRIRYAENETNYCPHCQTQDRVLADR
                     ALSRLLKDDWPRTVDELERMRGT"
     gene            10833..11054
                     /locus_tag="VNZ06_01925"
     CDS             10833..11054
                     /locus_tag="VNZ06_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19538.1"
                     /translation="MSVGRMLGVLLIAAGVLGLVYHGFSYTKESHDAKIGPLELSVRN
                     KETVDIPTWAGVAAIVVGAACLLVGNRKT"
     gene            11079..12446
                     /locus_tag="VNZ06_01930"
     CDS             11079..12446
                     /locus_tag="VNZ06_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19539.1"
                     /translation="MVPAVDRRTFGLAATAVLLTRLPWIGRDYGSDPDSYRVVISARH
                     LIRSGQYEASRLPGYPLYEYLTALSVNAAPWLSNLITALFSVAAFILFALILRSLQIP
                     RALWLAMAFAMVPVIYLSSCCTMDYIPALSFMLAAGYATLRARPVLAGVLLGLAVGCR
                     ITSGALLLPLCLWLLMSQGVRSGCKRSLELGATTLLTAAVCYAPVYQAYGTGFFTFFD
                     NDSYPSADVIFARALPQVWGVLGSLALLLWACALPVYHRYARQALSRPPVRHMLIVCA
                     TSILLYLVAFLRLPDEAGYLVPLVPWVLIAVGLLTPPRLVAALAFALLCSSWIGFQGL
                     TPSLQGPIVEDARVRESQREATVSVIDAVARLPGRAAVVSGWVLPRLMLALDGDRLGL
                     HRFLYLIEDDEDYRGYLADGWQIYYLPGVDLYESQAHHLDLSELGARELQVPKERQRP
                     ASSGE"
     gene            12514..12651
                     /locus_tag="VNZ06_01935"
     CDS             12514..12651
                     /locus_tag="VNZ06_01935"
                     /inference="COORDINATES: protein motif:HMM:NF028380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HWW19540.1"
                     /translation="MGKGDRKTRRGKIYRGSFGKSRPKDPTKAKKPSKTAKTSSSAPR
                     K"
     gene            complement(12671..13999)
                     /gene="hslU"
                     /locus_tag="VNZ06_01940"
     CDS             complement(12671..13999)
                     /gene="hslU"
                     /locus_tag="VNZ06_01940"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HWW19541.1"
                     /translation="MSPRAIVQELDKHIVGQGAAKRAVAIALRNRWRRMQVDEPMRQE
                     ITPKNILMIGPTGVGKTEIARRLARLAHAPFVKVEATKFTEVGYVGREVDSIIKELVD
                     VAIKMTREQQLSRVREGAREAANERVLDALLPKPRMLGFSTDETHPPRDADTRNKFRD
                     MLKRGELDEREIEIEVRAVPMGVEIMAPPGMEEMQQQLQSVLSGLGGNRTKTRRVKVR
                     EALKLMLEEEAAKLINEEELKTQALANAEQNGIVFIDEIDKIARRQETMGADVSREGV
                     QRDLLPLVEGCTVNTKYGQLKTDHVLFIASGAFHMSKPSDLIPELQGRFPIRVELDSL
                     KVEDFVRILTEPDASLTAQYRELLNTENVKLSFTSDGVRRIAEIACHVNDRTENIGAR
                     RLHTVMERLLETVSYDASEREDKTLSIDAQYVDQHLGSLAKDEDLSRYIL"
     gene            complement(14008..14553)
                     /gene="hslV"
                     /locus_tag="VNZ06_01945"
     CDS             complement(14008..14553)
                     /gene="hslV"
                     /locus_tag="VNZ06_01945"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HWW19542.1"
                     /translation="MNQAFDPHSTTILAVRRNDTVVLGGDGQVTLGNTVMKGNARKVR
                     RLSNSKVLAGFAGGTADAFTLFERFEGKLEKYGNLTRAAIELAKDWRADRYLRRLEAL
                     LLVGDPQNLFVISGNGDVIEPEYQAAAIGSGGPYAQAAARALIESTDLDARMIVERSL
                     GIAADICIYTNRNLVIEELRA"
     gene            complement(14577..>15468)
                     /gene="xerC"
                     /locus_tag="VNZ06_01950"
     CDS             complement(14577..>15468)
                     /gene="xerC"
                     /locus_tag="VNZ06_01950"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HWW19543.1"
                     /translation="SLQRLADFERYLSTERQLSPHTLQAYRRDLRAMQQWCTRQGVSE
                     WGVLDHQHIRQFAAHCHAGGLDGRSIQRRLAALRSFFTFLLREGALRHNPAIDVRAPK
                     AAKRLPNTLDVDQMARLLSFKPKNVLEIRDLALLELFYSSGLRLAELTSLTLPQVMFD
                     EATVRVLGKGRKERIVPVGSAALKALKRWLKERAALTGPDELHLFVGRDGAPLSERAV
                     QLRVARMARAAGVPQHLHPHMLRHSFATHLLESSRDLRGVQELLGHANIRTTQIYTHL
                     DFQHLARTYERAHPRAKRRR"
BASE COUNT         2872 a         4731 c         4864 g         3001 t
ORIGIN      
        1 ggctgcgaga actcgagtac cagcaattga ccgccgggct tgagcacgcg gtgaatcgag
       61 gccagagcgg cggttttgtc ggtcacattg cgcaatccga atccgatcgt tacgcaatcg
      121 aactgcgatg aggcgaatgg cagacgttcg gcatcggcct gcacgtactg gatattggcg
      181 atcagtcccc ggtcggtcag ccggtcgcga ccgcgcgcga gcatgctgcg gttgatgtca
      241 gacagcacga cccgtccgcg ctctccgacc tggcgagcca ttccggcagc caaatctccg
      301 gttcctccag ccagatccag tgcctgctga ccgtcgctca agccggtcag acgcagcgtg
      361 aagtgtttcc acagccggtg cgcgccgccc gacatcaggt cgttcatgag atcgtagcgc
      421 ccggacaccg agtcgaaaac tgcccgcacg cgcgttgctt tctccttcca gggcacctgc
      481 tggaagccga aatctgtatt gcccgacctg ccttcgctca tggggacccc ggtgaaaaac
      541 agtccgacat tctagggtcg gcgattggat gagtcataat gacggctgac aattgagcga
      601 gtaaaggact aatttagtct tatatgcccg agccgatatc gccgcatccg acagctagac
      661 tggttcaaaa atgctggcat ttgcgcccca gttcgtcgag aatccggctc cggttgctgg
      721 cccaaaaggt aagcagccga gtgggggtct tgtgaagatt tggccgggtg aagtcgcccg
      781 gcctttcttt tttgcgccga cagacggtac tcatccttaa gacgcgcgct cgacactgtc
      841 cgccctcagc accggcagcg gtcagcgccg atgtcccgag tgctcgacgc gcgccagata
      901 ctccgaccag agttgttcgc gctggcaccc aagctggtag agatacttcc acgtgtacag
      961 gccggtatcg tggccatcgt caaatgacag tttcaccgcg tattgcccga tcggctcgac
     1021 cgcgcgtatc ccgacctgct gcttgtcgag gaccagcacg cgctgatcgg gactgtgccc
     1081 ctgaacctca gcggaaggtg aatgcacgcg caggaattcg aacggcaact cgaaacggct
     1141 gccgtcgtcg aagctcacct cgagcactcg cgagcgctgg cgcagcttga tttctgtggc
     1201 ttgaacagtg ttcatggctg attcgcatcc tgacggttga tttgtaatac tgggaaatcg
     1261 gcggcgggct atgggtgagt atgaaatcga ccgacacctc taatcctgac tatttccatc
     1321 gcgtcgtgga ttgtcagtgg gcctgcccgg cccacaccga tgtacctgag tacatcagac
     1381 tcatcgccca aggtcgctat accgacgctt atatgttgaa tcgggaatcc aatgtgttcc
     1441 cgggaattct aggccgagtc tgcgatcggc cgtgcgagcc ggcctgtcgc cgcagccggg
     1501 tcgaagacaa accggtcgcc atctgccggc tgaagcgcgt cgccgcagac ctgaaagggg
     1561 agctcggcgg gttgctgccc aaagcgccgg cgatcaagaa cggcaagcgc attgcctgca
     1621 tcggtgccgg accggcctcc ctgacgatcg gcaacgatct ggcgccactg ggttatgagg
     1681 tcacgatctt tgaacagaac gccgtcccgg gcggactgat gcgcagcaac atcccttcgt
     1741 ttcggctgcc gcagtcagtg ctcgacgaag agattggaat gattctcgac atgggagttc
     1801 atctgaaact ctcctcgccg atcgaaagcc tcaagcagct gctggatgag ggcggcttcg
     1861 acgcggtttt cgtcggttcc ggcgcaccaa agggcaagga attgaagctg ccgggccgcg
     1921 accagaccga tcgcatacac atcggcatca cctggctcga gtcggtggca ttcggtcaca
     1981 tcggctcgat cggtgagcgc gtgctgatca tcggcgtggg taacaccgcg atggactgct
     2041 gccgcacgtc gctgcgcctc ggtgcgcaca gcgtcaaagt gatggcacgc aagccgcgcg
     2101 gcttcttcaa ggcatcggag tgggagctcg aggatgctga ggaagaaaac gtcgagatca
     2161 tcgtcaatca ctcgccgaag tcattcgtga tcgagcacgg caagctcacc ggcatgctgt
     2221 tcgacaagat ggaatacgat ctggaaaacg cagtcatcaa agccgagcgc ctggtgggcg
     2281 aagtgatgat cccgtgcgat gacgtgatcc tcgcgatcgg ccaggagaat gcgttcacct
     2341 gggtcgaacg cgacatcggc gtcaagttca acaagtggga tcagccggtg gtcgatgaga
     2401 ccacctttca gtccacccac tccaaagtgt tcttcggagg cgatgccgcc caggggccga
     2461 agaacatcat ttgggccgtc gagcaggcac accaggctgc aatctcgatc cacaactatt
     2521 gctgcggcgt tgcggtcacc gagcggccgg cgagccgcat gcagctctcc agccgcaaga
     2581 tgggcatgca cgagtgggcc tacaagaacg actatgcggc gctggagcgc cggctgatgc
     2641 cgcacgtgtc tttgaaggag cgcttcaaga agctgaacat cgaagtcgaa ctggggttca
     2701 gtgtcgagca ggccgctcag gaggcgcagc gctgtctcaa ctgcgacgtg cagacggtgt
     2761 tcgacgccaa gctgtgcatc gaatgcgacg cctgcatcga catctgcccg gtcgattgcc
     2821 tcacgatcac ccggggtggt gaggaagcgg acctgaagac gcggctgcgg gcaccggtgc
     2881 tggagccgac ccagctgctg tacgtctcgg atgcgctgcc gcagaccggg cgcgtgatgg
     2941 tcaaggatga gaatctgtgc gtgcactgcg gcctgtgcgc cgagcgctgt cctaccgcag
     3001 cctgggatat gcagaaatcg accatccact ggccgcacgc cgacgaaggg gggcccaagt
     3061 gccaaccgca aaagctcaag accgcgtaaa cgacttcgcc gtcaagatcg cgaccgtcaa
     3121 tggcaccggc tcggccagtg ccaacggctt gttgatgaaa gcgatctttc gtagcggcgt
     3181 gccggtggtc ggcaagaatt acttcccgtc gaacattcag ggtctgccga cctggtatga
     3241 aatccggatt acgcgcgacg cccatcgcgc gcgctccggg gcggtcgata tcatggtcgc
     3301 gctcaacgcc gagacctatg cgcgcgacct ggccgaggtc gcctcgggtg ggtacttcat
     3361 ttacgactcg acctggccca ggccggcgct gctgaagcgc gatgacatca cggtgatcgg
     3421 cgtgccattg gcgcagctgt gcaacgatgc cttccagggt gtccgcacgc gcatcctgat
     3481 gaagaacatc tgctacgccg gcgtactggc tgcgttgtgc cagctcgatc tggacgtgat
     3541 ccgcgatctg ctcggtgaga cctacgccaa gaagccgcaa ctggtggatg ccaacatgaa
     3601 ggcgatccag ctcggctacg actacgcgcg cgcgaacttc gcctgtccgc tcgggctgcg
     3661 tgtcgaaagg atgaatgcga cggccggcca catcatcatc gatggcaaca ccgccgcggc
     3721 gctgggctgc gtctacgccg gggcgacggt tggcgcgtgg tacccgatca cgccatccac
     3781 ctcgctgatg gatgcctaca agagcttctg cgatcgcctg cgtgttgatc cggtcaccaa
     3841 gcgcaagaac tacatcatga tccaggccga agacgaactg gcctcgatcg gcatggtgat
     3901 cggtggctgc tggaatggcg cgcgcgcctt taccgccacc tccggccccg gcatttcact
     3961 gatgcaggag ttcctagggt tggcctacta cgcggaagtg cccgcggtgg tgttcgatat
     4021 ccaacgcgtc ggcccgtcca ccggaatgcc gacccgaacg cagcagtgcg acatccacag
     4081 cgccgcttac gcttcacacg gcgatacccg gcaggtgtgt ctgtatccgg cgagccccga
     4141 ggagtgcttc gagtttgcgc cgaaggcatt cgatctggcc gagcggctgc agacgcccgt
     4201 gctggtgttg tcggatctgg acatcggcat gaacgattgg atgtgtccgg agttcaagtg
     4261 ggatgaaagc tatgtgccgg accgaggcaa agtgctggat gtcgagcagg tgaagcagct
     4321 cgagaagttc tggcgctact acgatcacga caacgatggc attgcgcagc gcacgctgcc
     4381 tggcacccac ccgaacgggg cgtatttcgc tcgcggctcg ggtcacaacc agtacggtgg
     4441 ctataccgag gactcaaagg aataccagat cgtcgtggac cggctgcgca agaagtggat
     4501 cacggcgtcg catctggtgc cgaaagcgat catcgagcgc aacgagaaga tcgatatcgg
     4561 cattgtctcg ctcggcagca gtgacggagc cgtgcgcgaa gcgcgcgcac ggctggaggc
     4621 gcagggaatt cataccaact acatgcgcat ccgcggcttt ccgttcggcg aagaggtcga
     4681 agcatttctg caggcccagc gcttgagttt tgtggtcgaa cagaaccgcg acgcgcaact
     4741 gcggtcgttg ctgaccctgg agacgggtat cgagaagaca cgactgcgat cgatcctgca
     4801 ctacaacggc atgccgattc cggcgagctg cgtgtatgac ggagttgcgg ccgtgatcac
     4861 gccgaatcgc ccggcggcgc agctcgtgag ctgacgagcg agggagggac tgatatgact
     4921 ttcatggcca agccgaagat cgtgcagctc gaactgccaa agaacgcact cggcctcacg
     4981 cggcgcgact atgaaggcgc caactcgacc ctgtgcgccg gctgcgggca cgattcgatc
     5041 accgccgcca tcgtcgaggc gtgctgggag agtgcgctgc gtccggagaa catcgtcaag
     5101 ctctccggca tcggctgctc ctccaagacc actgcatatt tcgtcagggg tggccacggt
     5161 ttcaactccg tgcacggtcg catgccctcg attgccacag gcgcgaacgc tgccaaccgt
     5221 gagctgacct acatcggagt ttccggtgac ggcgatacgc tctcgatcgg cctgggacag
     5281 ttctgtcatg cgatccgtcg caatctgaac atggtgtacg tcatcgagaa caatggcgtc
     5341 tacggactga ccaaggggca gttctcggcc tctgcggatg tcggcagcac cgcgaagaag
     5401 ggcgaggtga ataaccagcc gccgatcgat ccggtgcagc tggcgatcac tctcggtgtg
     5461 tcgttcgtgg ctcgtagctt ctccggcgac aaggcgcagc tcgtgccgct gctgcaggca
     5521 gcgatgcatc atcagggatt ctccctgctc gatgtcatct caccgtgtgt gaccttcaac
     5581 gatcacgaag gctcgactcg cagctacgct tacacccgcg agcactacga cgaggtcgtg
     5641 cacgctgact tcgtgccgcc ggcgcaggaa atcgcggccg attacgcgcc gggcgagacg
     5701 catgccgtca cgatgcacga cggcagccga gtgttgctgc gaaaggtgga acaggactac
     5761 gaccccactg accgaggcgt cgcgctcgac atgatccagc ggcgtttgaa gcagggtgag
     5821 tatctgaccg ggttactgta tatcggcagg gaccaggcgg agctgcatca gctcaatgca
     5881 acaccggacc tgccgctgaa cagactgccc tacgcccagc ttaaccccgg tccgcaggcg
     5941 ctggcgaaga cgctggcgag gtatcgctga tcaagtgggc cgtatgaagt gggcaatcat
     6001 cgcggcggcg agcgccgtga tggtggctct gctgggtgtc gcggcctacc tggtacacac
     6061 tcgccgtcca agcatcctag aagtggccgc cttcggccat ctgcgtacct atcccgcgtg
     6121 gtggcgaccg aagggattcg tctatctgtt ctccggcgag cgaggctgga gcgcggtgga
     6181 cgagcagacc gcgcgtgatc tggcgcgctc cggcaacctg gtgttcgggg tcgatgcacc
     6241 cgaattcctg caagtgcaga accgtctgga cggctgcatt tacctgccgg gaatactcga
     6301 agactacagc cgcccctggc agcacagcgc ggatatggct cagtatctgg cgcccgcggt
     6361 tcttggttcg gacatcggcg caacgctggc ctatgaagca caggtgcagg ccccaccgac
     6421 ggccttcagt gccgtggtgg ccgtggatcc tgtgtccgcc gtctcgatgc ggcggccatt
     6481 ctgcgagcac ccggcagccg cgatgacctc gagcgaacag accatcaaga gcgagccgcc
     6541 gggcaggaac gtgccgctgc ggatcgtgct ggacagctcg gcgagcaagg ccgcacgcca
     6601 gttcgccagc agcattccag ggcagacacc gacgacggtc aaaaccgcac agccccgcta
     6661 tcgcagctac cggcaagcac tcgaagacat tcacacgcaa atgcagaaaa gtggcatcgc
     6721 cgatctgcca ttggcgatcg tgcctgcctc acaatcgggc tgcgccaacg gtttcgcggt
     6781 tctgtactcc ggggacggcg gctggcggga tctggaccgc agtctggccg acattctggc
     6841 ctccaaggga atgagcgtca tcggtgtgga tgtcttgcgt tactactgga aagacaagtc
     6901 tcccgatgtc gctgcccgtg acctgacacg gatgatccgg tattacggcg agcaatggca
     6961 ttgccacaaa gtggccctga tcgggttttc attcggggcc gacatcctgc cgttcgtcgc
     7021 cagccggacg ccgcctgatg tgcgatcgga gctggtgctg atgacactgc tgtcgcctga
     7081 gcgcagcacc gcatttgaga ttgagatgaa cggatggctc ggtggcaagc ccaaggccgg
     7141 cgtgcccatc ggaccggagg tccgaaagct cgacggtctg aaagtgcaat gcgtctacgg
     7201 ccgggacgaa tccgcggaca gcctgtgcac cgacagcagc gctggtccgt taaccgtcct
     7261 ggcaaagagc ggcggacatc attttgatca ggactacaac gaactggccg acgacattct
     7321 ggcagcgctg ccgaaatagc attgcgctcg gttgggtggt gttggccgca aatctggcag
     7381 gagttggggt gacgcacgct gcgacgcaaa gcacggcctg gcattttgcg caggtccacg
     7441 ggcatcgcat ctactacgca ctgcgcggcg atgggccgac gctggtgctg ttgcatggcg
     7501 gcggcgattc cggcgagcac tcgttcgagc atcagctcga tgttttcgcc gaacaccacc
     7561 acatcgttgc gccggatcag gttgggcagg gccgcacccc tgcggcgccc ggtcccttga
     7621 gttacaccgg gatgatggaa gataccgcag cattgctgac ggaaatgaaa ctcagacacg
     7681 tggatctggt gggtttcagc gatggcggca ttctggcgct gatgctggcg ctgcgtcatc
     7741 ccgaactggt gcgacgcgtc gtcatcagcg gcgtcaatgt gtcgcctgag gggttgaact
     7801 ccgaatcgcg cgaggggctg cgcgcggtgc aggcggacga tccgaagacc atcgatgaaa
     7861 agctggcgcg actgtggctc acgtcgccgg ccgaatccga gcttagcctt caattgttga
     7921 aaaatgtggg tcagccggtg ctggtggttt ccggcgatcg cgatgccatc acgctggaac
     7981 ataccttgca gatttttcac gctctgcccc gggctgagct gtgcattctg cccggtaccg
     8041 accatggtac tttctccgga cggccggagt ggcttaaccc aatcatcacc cattttctca
     8101 accgcgaagc cgatcccggt ccgacgccga aatagcaatc agtctgtagc gcttcatgat
     8161 cactcgttca cgcgcgcaaa ttacgtgaac aaatgatgaa ttcgcggaac ttagtgtcgc
     8221 gcctctccgt cagtcgacaa ggtcggtcga tccacggagg tctttgtgaa cccaccctat
     8281 ctgctggcgc agggatccgg tacgacgcgt ttaccgttac ggttccactc cactctgccg
     8341 cggctcaaac tggccgattc ggccacccga gcgatgatcg actttcaacg cgacccaccg
     8401 ctgaccgttg acgagagcta ctccctggag gaggcgatcg accgcatgtt ccggctggga
     8461 gtgcgtgctt tcctggtggt tcgcgacctc agcgtggtcg gtttgatgac agccagcgag
     8521 gcgacgcgct caacaccgca gcactcacgt gtcgcagacg cgatgacgcc gaccgacgac
     8581 gttcctgcga tcgactggca cacgctggcc gagtccaggg tgcgcgatct ggtggagatc
     8641 tttgatgcta ccggcgtcaa ctacctggtg gtgctggaga gtcacagcgc gtcgctcagt
     8701 tcggtccgcg gcttgatcca acacaaacgc ctgcaacgcc agctcagctc gccctggtca
     8761 ttgcgggcag cgggaatcta atgagaccat tgccgcgatg tcacgatgca gcaagggtgc
     8821 acgcgaagtg cttcaattca ggcgtgtcag gcgtcatctg ctcatggctg cgggggggtt
     8881 gtgggtgcac ggtgcgctgg cagcggccgt accactggct ttaggttctg cgcgaggagc
     8941 actcgtcgac gtccatcatc actggtccaa taccgcgttg ctgcagtggt ggaaccgcag
     9001 tgctgccacc gctggctggg cggctgacaa ggcgctggcg gcgatggacg ccgccggcgt
     9061 tcgcaccgcc atgctttccg tcacgcagcc gggcgtctgg aacagcagcg atggcgacgg
     9121 gtccgtccgg atcgcgcgca cgtgcaacga agccatggcg cgaatcacgc aagatcatct
     9181 cgggcgtttc ggatattttg ctgcggtggc gctgccaatg gtgccggcga gcgtcgcaga
     9241 gatcggctat gcgctggatg ccctcaaggc tgacggcatt ggtctgctgt ccagctatga
     9301 cggcagctat ctcggtgacc cgagcttcac gccggtgttc gaggagctca atgctcgtcg
     9361 cgccgtggtc tacgtgcatc ctgcagtgcc gctctgctgt gcaaatctgg tgccggagat
     9421 ccccgccagc gcgctggagg cgccgtcgga tacctcgcgc acgatcgaga gcctgctggt
     9481 cagcggtgcg ctcgcgcgct tcagtgccat ccggttcatc tttgcgcacg gcggcggcac
     9541 gctgccgttc ctggcggagc ggttactcga ctcactcgtg cccgaccccc cggccgacaa
     9601 gagctatctt tcgcgctaca acgcaaaggt cgcgctggcg cagctttatt tcgatagtgc
     9661 cagcgtggcc aatgcgccgg cgtgggcggc gctgacgagc tttaccacgc cggatcgaat
     9721 cctgttcggc agcgactacc cagcgcacga ggtgcgtgcc cagctcggca gtctgcgggc
     9781 gatggagcag cgctttggtg tggcactcgc ggatgcgcgg agcatcgaat ccggcaacgc
     9841 gcaacatctg ttcgcggcgc gcctgagctg aatctgccgc gatcgccatg ccggagctgc
     9901 ccgacgtcac ggtctacctg gaagccttga gcgagcggat catcgctcag cgcctcgatc
     9961 gcatcgcgct gatcaatccg tttctgttgc gcaccgcgac acccccattg aagagcgctg
    10021 aaagccggcg cgtcgaacgc ctgaggcggg tgggcaagcg catcgcgatc ggactgaccg
    10081 atgaactgtg gctggtgctg cacctgatga ttgccggtcg cctgcattgg tttgggcgcg
    10141 ggagcaaagt gtccggccgc gccgctctgg ccagatttga gtttgagagc ggtacgctga
    10201 cgttgaccga agcgggcagc aaacggcgtg cgtcgctgca cctgatcagc ggtgaatcga
    10261 acctcgcgcg gatggatccc ggaggtctgg aggtgctgga ggctgatctg gacgcgttca
    10321 ctgcgcgcct gcggctcgag aatcacacgc tcaagcgcgc gctcaccgat ccgcatctct
    10381 tcagcgggat cggcaacgcc tattccgacg agatcttgca tcgggcacgg ctgtcgcctc
    10441 tggcgctgac atcgcgcctc agtgcggatg aaatcgcccg gctctatgag gcgacgaggg
    10501 gttcattgac cgattggacc gaactgctgc gacaggaagc cggcggcaaa ttcccagaag
    10561 gagtcacggc attgcgtcca cagatggcgg tgcacggccg gtacggtcaa gcctgtccga
    10621 tatgcggcac caccgtgcag cgcattcgtt acgccgagaa tgaaacgaac tactgccctc
    10681 actgccagac gcaggaccgc gtgctggcgg atcgggcact ttcccggctg ctgaaggatg
    10741 actggccgag gaccgtggat gaactggaac ggatgcgcgg aacttagcac tgctacactg
    10801 cctgcatcac ctgacagcaa agggattagg gcatgagcgt aggaagaatg ctgggcgtct
    10861 tgctgatcgc cgcgggtgtg ctggggctgg tctatcacgg cttcagttac accaaagaat
    10921 cgcacgacgc gaagattggt ccgctggaac tgtcggtcag gaacaaggaa acggtggata
    10981 tcccgacctg ggcaggtgtt gcggccatcg ttgtcggagc ggcgtgtttg ctggtcggca
    11041 atcgaaagac ctgacggtcg ccgcactcga cgccgacgat ggtgccagcc gttgatcgca
    11101 ggacattcgg acttgcggcc acggcggtgc tgctcacacg gctgccatgg atcgggcgtg
    11161 actacggatc cgatccagat tcctatcggg tcgtcatcag cgcccgacac ctgattcgca
    11221 gtggtcaata cgaggcttca cgcctacccg gctatccgct gtatgaatat ctgaccgcct
    11281 tgagcgttaa cgctgcgcct tggctctcca atctcatcac cgctttattc agtgtggcgg
    11341 cgttcatact ttttgcactg atcctgcggt cactgcagat ccctcgagcg ctgtggctgg
    11401 ccatggcatt tgccatggta ccggtgatct acctcagcag ctgctgcacc atggactaca
    11461 ttcccgcact ctcgttcatg cttgcggccg gctacgccac gttgcgtgca cgacccgttc
    11521 ttgccggcgt gctgctgggc ctggcagtcg gctgccgcat cacctccggc gcgctgctgt
    11581 tgccgctttg cctgtggtta ctgatgtcgc agggcgtgcg ctctggctgc aaacgatcgt
    11641 tggaattggg cgctacgacg ctgctgaccg cggccgtgtg ctacgcgccg gtgtatcagg
    11701 cgtatggcac cggcttcttc acgttcttcg acaatgacag ctacccgtcg gccgatgtca
    11761 ttttcgcccg cgcgctgcct caggtgtggg gcgtgctcgg ctcgctcgca ttgctgctat
    11821 gggcgtgcgc gctgcctgtc tatcatcgat atgcacgaca ggcgctgagc agaccgccgg
    11881 tgcgccacat gctcatcgtg tgtgcgacct cgatcctgct gtacctggtc gcgtttctgc
    11941 gcttgccgga tgaagcggga tatctggtgc ctctggtgcc gtgggtgttg atcgcagtcg
    12001 gattgctgac gccaccacgt ctcgtcgcgg cgctggcgtt cgcgttgctg tgttcttcgt
    12061 ggataggctt ccagggactg acgccctcgc tgcaagggcc gatcgtcgag gacgcccggg
    12121 ttcgcgaatc gcagcgtgag gcgaccgtgt cagtgattga tgccgtggca cgcctgccgg
    12181 ggcgggcggc ggtggtctca ggttgggtac tgccgcggtt gatgctggca ctggacgggg
    12241 atcgccttgg attgcaccgc ttcctctatc tgattgagga cgatgaggac tatcgaggct
    12301 acctggcgga cggctggcaa atctactatc tgcccggcgt cgatctctat gagtcgcagg
    12361 cgcaccacct ggacctgtcg gagctgggcg cgcgcgaact gcaggtgccg aaggaacgac
    12421 agcgtccggc ttccagcggc gaataggctg gcaagcctgc ccgggaatca atagactgcg
    12481 cgcggaagtc tttcgcagca ggagtttccg acagtgggta aaggcgatcg caagacacgt
    12541 cgcggcaaga tctatcgcgg ctcgttcggc aagtcgcggc cgaaggatcc aacaaaggcg
    12601 aagaagccga gcaagaccgc caagacctcg tcctccgcac cgcgcaaata ggccgcggac
    12661 cccgagccgc tcaaagaatg taacgactca gatcctcgtc cttggccaga gatcccaggt
    12721 gctgatcgac gtactgcgcg tcgatgctca gagtcttgtc ctcacgctcg ctggcgtcgt
    12781 aggaaacggt ttccagtagc cgttccatga ccgtatgcaa gcggcgagcc ccgatgttct
    12841 cggtgcggtc gttgacgtga caggcgatct ccgcgatccg gcgcacaccg tccgacgtga
    12901 agctgagctt aacgttctcg gtattcagca gctctcgata ttgcgcggtc agcgacgcgt
    12961 ccggctcggt cagaatgcgg acgaagtctt ccaccttcag cgaatccagt tcgacgcgga
    13021 tcggaaaacg tccctgcagc tccgggatca gatcggaggg cttggacatg tgaaacgccc
    13081 ctgaggcgat gaacaacaca tgatcggtct tgagctggcc gtacttggtg ttcacggtgc
    13141 agccttcgac caacggcagc aaatcgcgct gtacaccttc acgcgagaca tctgcgccca
    13201 tggtttcctg gcggcgcgcg atcttgtcga tctcgtcaat gaacacgatg ccattctgct
    13261 cggcgttggc gagagcctgc gtcttcagct cctcttcgtt gatgagcttc gcggcctctt
    13321 cctcgagcat cagtttgagc gcttcacgca ccttgacgcg gcgcgtcttg gtgcgattgc
    13381 cgcccaggcc tgacagtacc gattgcagct gctgctgcat ctcctccatg cccggtggcg
    13441 ccatgatttc cactcccatc ggcaccgcgc gcacttcgat ctcgatttcg cgctcgtcga
    13501 gttcaccgcg cttgagcatg tcccggaact tgttgcgtgt gtccgcatcg cgcggcggat
    13561 gagtctcgtc ggtcgagaaa ccgagcattc tcggcttggg taacagtgcg tcgagcacgc
    13621 gctcgttggc cgcttcacgc gcaccctctc gaacgcgtga caattgctgc tcgcgggtca
    13681 tcttgatcgc aacgtcgacc agctccttga tgatcgagtc cacttcccga ccgacgtagc
    13741 cgacttcggt gaacttggtg gcctcgacct tcacgaacgg tgcgtgggcc agtcgcgcca
    13801 ggcgccgggc gatttcggtc ttgccgacac ccgtcggtcc gatcatcagg atgttcttcg
    13861 gagtgatttc ctgacgcatc ggctcgtcga cctgcatacg cctccagcga ttgcgcaacg
    13921 caatcgcgac cgcacgcttg gcggccccct gaccgacgat gtgtttgtcc agttcctgca
    13981 cgatagctcg cggactcatg tcagcactca tgcccgcagt tcctcaatca cgaggttgcg
    14041 attggtatag atgcagatat cagcggcgat accgagcgag cgctcgacga tcatgcgtgc
    14101 atcgaggtcc gtgctctcga tcagcgctcg cgcggccgcc tgtgcatacg ggccgcctga
    14161 gccgatcgcg gccgcctggt attcaggttc gatcacatcg ccgttgccgg agatcacgaa
    14221 cagattctgc ggatcaccga ccagcagcag cgcttcgaga cgccgcagat agcggtcggc
    14281 acgccagtcc ttggcaagct cgatcgcggc gcgcgtcagg ttgccgtatt tctcgagctt
    14341 gccctcgaag cgctcgaaca gcgtgaaagc atccgccgtg ccgcccgcaa agcccgcgag
    14401 caccttgcta ttggacagcc ggcgcacctt gcgcgcattg cccttcataa cagtattgcc
    14461 gagcgtgacc tggccatcac cgccgaggac cacggtgtca ttgcgccgga ctgccagtat
    14521 cgttgtggaa tgggggtcga atgcctggtt catcgagaat tccttggggc aaaacctcac
    14581 cggcgccgct tggcgcgcgg atgggcccgt tcatatgtgc gggccagatg ttggaaatca
    14641 aggtgcgtgt agatctgcgt ggtgcggatg ttcgcgtggc ccagaagttc ctgcacaccc
    14701 cgtaaatcac gactggattc aagcagatgc gttgcgaagg aatgccgcag catgtgcggg
    14761 tgcaggtgct ggggaacccc cgcagcgcgc gccatgcggg ccacgcgcag ctgtaccgcc
    14821 cgttcgctca atggcgcgcc gtcacgtccg acgaacaggt ggagctcatc cggcccggtg
    14881 agcgcggcac gctctttgag ccagcgtttg agcgccttga gggccgcgga gcccaccggc
    14941 acgatgcgct ccttgcggcc tttgccgagc acccgtaccg tcgcttcatc gaacatcact
    15001 tgcggcaacg tcagcgaggt caattccgcc aggcgcaggc cgctcgaata gaacagctcc
    15061 aacaacgcga ggtcgcggat ttccaggacg ttcttcggct tgaaggacag cagccgtgcc
    15121 atctggtcca cgtccagggt gttgggcaat cgcttggctg ccttcggagc ccgcacatcg
    15181 atcgcagggt tgtgcctgag cgcaccttcg cgcagcagga acgtaaagaa gctgcgaagt
    15241 gcggccagcc gtcgctggat gctgcgccca tcgaggccac cggcgtgaca gtgtgcggcg
    15301 aactggcgga tgtgctggtg atccagcacg ccccattcgc tcactccctg ccgagtgcac
    15361 cactgctgca tcgcccgcag atcgcgccga taggcctgca acgtatgcgg cgagagctgc
    15421 cgctcggtgc tcagatagcg ttcaaagtct gccagtcgct gcagcgac
//
LOCUS       DATMRM010000064         5977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3124869, whole genome shotgun sequence.
ACCESSION   DATMRM010000064 DATMRM010000000
VERSION     DATMRM010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3124869"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VNZ06_01955"
     CDS             <1..654
                     /locus_tag="VNZ06_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19544.1"
                     /translation="NRAQITRAVSLVLGGRQSLCYTLGMAIMFGSILSYVGMVQQIFQ
                     DVFRRPGLMPGVFALCASSMAVMSFLNSRIVERFGMRAISQTGLFVFIGMTALHVLVI
                     GLGLERLWTFVLLQAVTMSCLGLTGSNFGAMALEPVGSVAGVGASLQGFISTTGGAVV
                     GAVTGKWFSGSTMPLALGALLCGLSSLALVVYAERGRLFNPHHSDPAPPRVAAETPG"
     gene            complement(596..1495)
                     /locus_tag="VNZ06_01960"
     CDS             complement(596..1495)
                     /locus_tag="VNZ06_01960"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HWW19545.1"
                     /translation="MSGSFLVHRLRDQTYWLYLPTTPEPSAPLPLIVMLHGCGQTAAE
                     FAQGSRMNQYAWCAILYPEQNRAANSMRCWNWFEPEGLDGRADCALIVQLIRDAQARY
                     PINPAHCYVVGFSAGAAMAAVLCSTHPDLFAGCALHSGLMAQAATSLSQAVACMRHGV
                     DPEAFNRSVQRMRARLSAGAHTAPTLVIHGSADLIVNPVNAQQIAEQTRVLNTRLNGS
                     VEPLFSSERWTDSPGRPYRQQEMTLGDKLLLRTLIIEGLGHAWSGGDARHEYFDPVGP
                     DASRLILEFLLPPAAAPGPNDAG"
     gene            complement(1504..3840)
                     /locus_tag="VNZ06_01965"
     CDS             complement(1504..3840)
                     /locus_tag="VNZ06_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWW19546.1"
                     /translation="MEQLRDDAARPKPALGLMDTATLRIFVNSVRDYAILMLDPTGHI
                     MSWSPGAEAITGYAPHEIIGAHVLRFYPPEAIAAGLPERELSIASQTGRFEDEGWRVR
                     KDGSKFWADVVVTALRDEQRELIGFAKVTRDLTERRRTEEALRHNEERFRSLIEGVKD
                     YAIFMLDTKGFVSTWNAGAKQINGYEASEIIGSHFSRFYPPEALKRGLPEHELRTATV
                     DGRFEDEGWRLRKDGSRFWANVIITAMRDASGTLTGFSKITRDLTLRKAQEERLRMSE
                     ERFRLLVDGVTEYALIMLDENGIVTSWNAGAQRIKGYRSDEILGKHYSHFYTPEDVAA
                     NKPWQQLARARVEGRVTDEGWRVRQDRSLYWAGTTITALHDADGRLHGFAKMTQDLTE
                     RRRSETLVDSTQRMHEFIAMLAHELRNPVAPIRNAVALMGRKGIHDPTLEAMRQTIDR
                     QSSHLARLLDELLDVNRVARGEFSIQKEMLDLQDVLTRSIESSQPVIDAHGHRLHVER
                     ADAPLYVSGDPTRLTQAFVNILNNAAKYTPDGGDITLRVSADETEVEIRISDTGDGIA
                     PEYLERVFELYVQIDPNKNGTLGGLGVGLALVRRVIELHAGHVYARSAGLGKGSEFVV
                     RLPRSTQGLRAVELAAGPEPGARGSLQVLVVDDNRDAADTLTALVQSMEHSCQTVYDG
                     ESALRLAGTLKPDVIFMDIGLPHVSGYQIAQELRRRALTPRPLLVAVTGWGQEADRRL
                     AIQSGFDLHFAKPVSERTIRAVLSEACGKDEAAEIHLH"
     gene            complement(4043..>5977)
                     /locus_tag="VNZ06_01970"
     CDS             complement(4043..>5977)
                     /locus_tag="VNZ06_01970"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HWW19547.1"
                     /translation="EVVQYVYRKYGRERAALAATVICYRTRSAVRDVGRALGISDEDL
                     NRLSRMHVWWDPPGEIQRKFASTQLRIDAERLQRMMPLVRQLIGFPRHLSQHVGGFVI
                     SGEPLSALVPVENAAMPDRTVIQWDKDDLESLGLLKVDVLALGMLTALHRCFDLIEGY
                     SGRRLRVQDIPPKDPATFDMICRAETIGVFQIESRAQMSMLPRLKPRNFYDLVIEVAI
                     VRPGPIQGDMVHPYLQRREHPESVSYPSEALREVLKRTLGVPIFQEQVMQIAVVAADF
                     TPGEADGLRRAMAAWKRRGGLEPYRERLMAGMAKNGYAPEFAEQIYRQISGFGEYGFP
                     ESHAASFALLTYISSWLKCHEPAAFAASLINSQPMGFYAPAQITQEVQRNGVKILPAD
                     VTCSHWDCTLIPAQNPASQPTLRLGLRLVRGLKRSDADAVLAKRALRPFASIDELAAR
                     ALLSRPAIQALAAAGALSALSAHRHDANWQALGVERLPGMIAGTSARERQVALPVPTE
                     SQDILADYRHLGLTTGRHPLALLRASLIRRGFRSSADLQQLADGTRVRVSGLVTHMQQ
                     PATASGVVFASLEDETGIVNIILWPKVFAAQRQCALESSLLVVEGTLQRRDLVAHVVA
                     KRLHNASDWLGLTRHSRDFH"
BASE COUNT          982 a         1913 c         1950 g         1132 t
ORIGIN      
        1 aatcgtgcgc agatcacgcg cgccgtatct ctggtgctcg gcggccggca gtcactgtgt
       61 tacacgctgg gaatggcgat catgttcggc tcgatcctgt cgtacgtcgg catggtgcag
      121 cagatcttcc aggacgtgtt ccgccggccg ggtttgatgc ccggcgtgtt tgcactgtgc
      181 gcctcctcga tggcggtcat gtcgtttctg aactccagaa tcgtcgagcg cttcggcatg
      241 cgggcgatct ctcagaccgg gctgtttgtt ttcatcggca tgacggcgct gcatgtgctg
      301 gtgatcgggc tcggcctgga gcggttgtgg acgttcgtgc tgttgcaggc tgtcacgatg
      361 tcatgccttg ggttgaccgg ctcgaacttc ggtgcgatgg cgctggagcc ggtgggttcg
      421 gtcgccggcg tcggtgcgtc gctgcaggga ttcatcagca ccacgggcgg tgcggtggtg
      481 ggtgctgtga ccggcaaatg gttcagtggc tcgaccatgc cgctggcgct gggcgcgttg
      541 ctgtgtggac tgagcagtct ggcactggtg gtttacgcgg aacgcggccg attgttcaac
      601 ccgcatcatt cggacccggc gccgccgcgg gtggcagcag aaactccagg ataagacggc
      661 tcgcatccgg cccgaccgga tcgaagtatt cgtgccgtgc atcgccaccg ctccaggcat
      721 ggcccaggcc ttcgatgatc agggtacgca gcagcagctt atcgcccaag gtcatctcct
      781 gctggcgata gggtctgccg gggctgtcgg tccagcgctc gctgctgaac agcggctcga
      841 cgctaccgtt cagtcgggtg ttcagcactc gcgtctgctc ggcgatctgc tgcgcattga
      901 ccggattgac gatcaggtcc gcggatccgt ggatcaccag cgtcggcgcg gtatgcgcgc
      961 cggctgacag tcgcgcccgc atccgctgca cgctgcggtt gaaggcctcg gggtcaacac
     1021 cgtgccgcat gcacgccacg gcttgcgaca ggctggttgc cgcctgtgcc atcagtcccg
     1081 aatgcagtgc gcagccggca aataaatccg gatgggtcga acacagcacc gccgccatcg
     1141 cggcgcccgc ggaaaagccg acgacgtagc agtgcgccgg attgatcgga tagcgcgcct
     1201 gcgcgtcccg tatcagctgc acgatcagcg cgcagtcggc gcggccgtcg agaccttcgg
     1261 gctcgaacca gttccagcag cgcatcgaat tggcggccct gttctgctcg ggatacagaa
     1321 tcgcgcacca ggcgtactgg ttcatgcggc tgccctgggc gaactcggcc gcggtctggc
     1381 cgcagccgtg cagcatcacg atgagtggca ggggagcgga cggctcaggc gtcgtgggta
     1441 ggtagagcca gtaagtctga tcgcgcagtc tgtgaacgag aaagctaccg gacatgaagc
     1501 ccgctagtgt aggtgaatct cggctgcctc gtctttgccg caagcctcgc tcagcacggc
     1561 acggatggtt ctttcgctga ccggctttgc aaagtgcaga tcgaatccgg attggatcgc
     1621 aaggcgccgg tccgcctcct ggccccagcc tgtcaccgcg accagcagcg gtcgcggagt
     1681 gagtgcgcgg cggcgcagct cctgcgcgat ctgatagccg ctgacgtgcg gcagcccgat
     1741 gtccatgaag atcacgtcgg gtttgagcgt cccggccaac ctcaatgccg actcgccgtc
     1801 atagacggtc tgacagctgt gttccatcga ttgcaccagc gcggtgaggg tatcggccgc
     1861 atcacggttg tcgtccacca ccagcacctg cagactgcca cgcgcgccag gctcgggccc
     1921 ggccgcgagc tccacggcgc gcagcccctg cgtcgagcgt ggcaggcgca ccacgaattc
     1981 gctgccctta ccgagtccgg cgctgcgcgc gtagacatgg ccggcgtgca gttcgatcac
     2041 ccgtcgcacc agcgccaggc caacgcccag accgccgagc gtgccgttct tgttgggatc
     2101 gatctgcacg tacagctcga acacgcgctc caggtactcg ggcgcgatgc catcgccggt
     2161 atcgctgatg cggatctcca cttcggtctc atccgcggac acgcgcagcg tgatgtcgcc
     2221 gccgtccggg gtgtacttgg ccgcattgtt caggatgttg acgaacgcct gggtcaggcg
     2281 cgtcggatcg ccgctgacgt acagcggcgc gtccgcgcgt tcgacgtgca ggcggtggcc
     2341 gtgcgcatcg atgacgggct gactcgattc gatcgagcgc gtcagcacgt cctgcagatc
     2401 cagcatctcc ttctgaatgc tgaattcgcc gcgcgccacg cggttgacgt ccagcagctc
     2461 atccagcagc cgtgccagat gtgaggattg ccggtcgatg gtctgtcgca tcgcctccag
     2521 cgtcggatcg tggatgccct tgcgtcccat cagggccacg gcattgcgga tcggggcgac
     2581 cggattgcgc agctcgtgcg ccagcatcgc gatgaactcg tgcatgcgct gcgtactgtc
     2641 caccagagtc tcgctgcgcc ggcgctcggt cagatcctgc gtcatcttcg cgaagccgtg
     2701 cagacgcccg tcggcatcgt gcagggcggt gatcgtggtg ccggcccagt agagactgcg
     2761 atcctggcgg acgcgccagc cctcatcggt cacccggccc tcgacccggg cgcgcgccag
     2821 ctgctgccac ggcttgttcg ctgccacgtc ctcgggcgta tagaagtgcg agtagtgctt
     2881 gcccagaatc tcgtcggagc ggtagccctt gatgcgctgc gcgccggcgt tccagctggt
     2941 cacgatgccg ttttcatcca gcatgatcag ggcgtattcg gtgacgccgt ccaccagcag
     3001 gcggaagcgc tcctcgctca tgcgcagccg ctcctcctgc gctttgcgca gcgtcagatc
     3061 gcgtgtgatc ttggaaaaac cggtcagcgt gccggacgca tcgcgcatgg cggtgatgat
     3121 gacgttggcc cagaatcgcg agccgtcctt gcgcagccgc cagccctcat cctcgaagcg
     3181 cccatcgacg gtcgcggtgc gcagctcatg ctccggcagc ccgcgcttga gtgcctccgg
     3241 cgggtagaag cgcgagaaat gcgaaccgat gatctcgctg gcttcgtagc cattgatctg
     3301 cttggcgccc gcattccagg tgctgacgaa gcccttggtg tcgagcatga agatcgcgta
     3361 gtccttgacg ccttcgatca gggagcggaa gcgctcttcg ttgtgacgca gggcctcttc
     3421 ggtccggcgg cgctcagtca ggtcgcgcgt gaccttggca aatccaatca gctcgcgctg
     3481 ctcatcgcgt agcgccgtga cgaccacgtc ggcccagaac ttcgagccgt cctttcgcac
     3541 ccgccagcct tcgtcttcga agcgtccggt ctggctggca atcgacagtt cgcgctccgg
     3601 caatcctgct gcgatggcct ccggcgggta gaagcgcagg acgtgcgcgc cgatgatctc
     3661 gtgcggggcg tagccggtga tggcctcggc gccagggctc cagctcatga tgtggccagt
     3721 ggggtcgagc atcagaatgg catagtcacg gacgctgttg acgaagatcc gcagcgttgc
     3781 cgtatccatc agcccgagcg ccggcttggg gcgtgcggcg tcgtccctca attgctccac
     3841 tgatgcttgc tccgagaggg cccgagaggg cccgaaggcc tcgctgatgc acccattctg
     3901 aacaaccgcg gggacggaaa cgactactgt cggcacccga agtgtgtcag cgcagtcgta
     3961 cagctgttgc tcgtcaggcg tcactgggcc tgcgggcctt ggcctactac tggaaagtac
     4021 tgtcctcaag cgctcgtggt cgctaatgaa agtcgcgtga gtgacgcgtc aggccgagcc
     4081 aatcggaagc gttgtgcaga cgcttggcta ccacatgcgc gaccaggtcg cgccgctgca
     4141 gggttccttc gaccaccagc agactggatt cgagcgcgca ctggcgctgg gccgcaaata
     4201 ccttcggcca cagaatgata ttgacgatcc cggtttcatc ttccagcgag gcgaacacca
     4261 cgcccgaagc ggtggccggc tgttgcatgt gagtgaccag gccgctcacc cggacacggg
     4321 taccgtccgc gagttgttgc aggtcggcgc tgctgcgaaa gcccctgcgg atcagcgatg
     4381 cgcgcagcaa cgccagcgga tgacggccgg tggtgagccc cagatggcga tagtcggcga
     4441 ggatgtcctg actttcggtg ggcaccggca gagcgacctg ccgctcgcgc gctgacgttc
     4501 cggcgatcat gcccggcagg cgttcaacgc ccagcgcctg ccagttggca tcgtgccgat
     4561 gtgcgctcag cgcactcagg gcgccggctg cagccagcgc ctgtatggcc ggtcgcgaaa
     4621 gcagggcgcg cgctgccagt tcatcgatac tcgcaaacgg ccgcagtgcg cgtttggcca
     4681 gcacggcgtc ggcatcggag cgcttcagtc cgcgtaccag ccgcaggcct aagcgcaatg
     4741 tcggttgcga ggccggattc tgcgccggga tgagcgtgca gtcccagtgg ctgcaggtga
     4801 cgtccgccgg cagaattttc acgccgttgc gttgtacttc ctgcgtgatc tgggccgggg
     4861 cataaaaacc catcggctga ctgttgatga gcgacgcggc gaacgcggcc ggctcgtggc
     4921 atttgagcca ggaggaaata taggtgagca gggcgaacga ggccgcgtgc gattcgggaa
     4981 agccgtattc gccgaagccg gagatctgcc ggtagatctg ttcggcgaat tcgggcgcat
     5041 agccgttctt cgccatgccg gccatcagcc gctcgcgata gggctcgagc ccgccgcgtc
     5101 gtttccatgc ggccatcgcc cgtcgcaggc cgtcggcctc gccgggtgtg aaatccgccg
     5161 cgaccaccgc gatctgcatc acctgttcct gaaaaatcgg tactccgagg gttcgtttca
     5221 gtacttcccg caaggcctcg ctcggatagc tcaccgactc ggggtgttcg cggcgctgta
     5281 agtagggatg caccatgtcg ccctggatcg ggcccggccg cacgatcgcg acttcaatca
     5341 ccagatcgta gaagttgcgg ggtttcagac gcggcaacat gctcatctgg gcgcgcgatt
     5401 cgatctgaaa cacaccgatc gtttcggcac ggcagatcat gtcgaaggtg gccggatcct
     5461 tgggcgggat atcctgcacg cgcagtctgc ggccgctata accctcaatc agatcgaagc
     5521 agcgatgcag cgcggtcagc atgcctagag ccaatacatc gaccttcagc aatcctaagg
     5581 attccagatc gtccttgtcc cattgaatga ccgtgcgatc cggcatggcc gcgttttcga
     5641 ccggcaccag cgcgctcaag ggttcccccg agatcacgaa accgcccacg tgctgcgaca
     5701 gatgccgggg gaaaccgatc agctgacgca ccagcggcat catcctctgc agtcgctcgg
     5761 cgtcgatgcg caactgcgtg ctggcgaact tgcgctggat ctcgcccggc ggatcccacc
     5821 acacgtgcat cctgctcagg cgattgagat cctcgtcact gatgccgagt gcgcgtccga
     5881 catcgcgtac cgcgctcctg gtgcgatagc agatgacggt ggcggccagc gcggcacgtt
     5941 cgcggccata tttgcgatac acgtactgca cgacttc
//
LOCUS       DATMRM010000065         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_956435, whole genome shotgun sequence.
ACCESSION   DATMRM010000065 DATMRM010000000
VERSION     DATMRM010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956435"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VNZ06_01975"
     CDS             complement(<1..487)
                     /locus_tag="VNZ06_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19548.1"
                     /translation="MRRAITGRQSFTGRGALSNPPGRFDLQTLEAVDDGWYLEESPDS
                     IATTLEPERARTVISHNDSPDIPFDRSINPYRGCEHACSYCTAGDTAVLMADGSTRPL
                     YQLKVGHEIYGTRRVGHYRRFVKSRVLAHWSTIKPAFRTTLEDGTTLVTSGDHRFLTE
                     RG"
     gene            complement(599..979)
                     /locus_tag="VNZ06_01980"
     CDS             complement(599..979)
                     /locus_tag="VNZ06_01980"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HWW19549.1"
                     /translation="MSDPNSSPASGAAPTENERTWGMLAHLSALVGLVIPLIGNVLGP
                     LGVSIARADQSAFVAAHAKEALNFNISVTLAAVVCALLMLVFIGFLLGSALFIAWLVM
                     TLIAAIKASEGVAYRYPFSLRLVK"
     gene            complement(1052..>2142)
                     /locus_tag="VNZ06_01985"
     CDS             complement(1052..>2142)
                     /locus_tag="VNZ06_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWW19550.1"
                     /translation="PAAQQSASDTDEYYGRATAHLLAPDGGLNQTLGVAFTRNRTATL
                     QPQTPESLNTGERVKLDWQGVIRVSSAHSVLLGLEDARDEISAPISASMRIASAYVEL
                     QSQLGEHWFSALNARYDDNDRFGSKVTYRIAPTWVSSASGTKLKASVGSGFKAPTLSE
                     LFQSFPPFFFANPDLKPESSVGYDLGIEQGLGGGVVRIGATWFYNRIRDLITTDVTGT
                     TYANVGRASTDGVEGFVAYQPVKPLTLRLDYTYTEATDEVLHQELLRRPKHKGNLNAT
                     WQATDALLLNASVLTVSSWVDGNRDFSIPRLDAPGYTTVNLAASFDLSRQLAVFGRID
                     NLFDRHYENPVGFLQPRMGVFAGIRAKL"
BASE COUNT          384 a          674 c          695 g          389 t
ORIGIN      
        1 aaccccgctc cgtgagaaaa cggtgatcgc cactcgtcac gagtgtcgta ccgtcctcca
       61 gcgtcgttcg gaaagccggc ttgatggtgc tccagtgcgc gagcacgcgg gattttacga
      121 atcgccgata gtgaccgacg cgccgggtgc cgtagatctc gtgcccgacc ttcaactggt
      181 acagaggccg cgtgcttcca tcagccatga gaacagctgt gtctccagct gtgcagtaag
      241 agcacgcatg ctcgcatcct cgatacggat tgatagaacg atcgaaagga atgtcgggcg
      301 aatcgttgtg gctgatcacc gtgcgcgcac gctcgggctc gagcgtcgtg gcgatgctgt
      361 cgggtgactc ctcgagatac cagccgtcgt ctaccgcctc gagtgtttga agatcgaagc
      421 ggcctggagg attggacagg gcgcccctgc ctgtgaacga ctggcggccg gtgattgcgc
      481 ggcgcatggt ggtactgtat atatatacag atcgcccgta aagccgccga cggtaatccc
      541 aaaaaacgcc gcagcggcgg gcacttagcg cccgcgcagc gcgctcagct tgcccgcgtt
      601 atttcaccag ccgcagcgag aacggatagc gatacgcgac gccctcgctc gccttgatgg
      661 cggcgatgag cgtcatgacc agccaggcga tgaacagcgc gctgcccagc aggaagccga
      721 tgaacaccag catcaggagc gcgcatacga ccgcggcgag cgtgaccgag atgttgaagt
      781 tgagggcttc cttggcgtgc gcggcgacaa aggccgactg atcggcccgg gcgatcgata
      841 ctcccagcgg ccccaacacg ttgccgatca gcggaatgac cagtcccacc agcgccgaca
      901 ggtgcgcgag catgccccag gtgcgctcgt tctccgtggg tgcggcgccg gaggcaggac
      961 tgctgttcgg gtcgctcatg gcggacccaa gattatcact tcgctgcggg cggggccgtc
     1021 ctgacaccga gcctgccgct gaccgttgga actacagctt cgccctgatg cccgcaaata
     1081 ctcccatgcg cggctggaga aacccgactg gattctcgta gtgccggtca aaaagattgt
     1141 cgatgcgccc gaacacggcc agctggcggc tgagatcgaa actcgccgcg agattcacgg
     1201 tggtataccc gggcgcgtcg aggcgcggga tcgaaaagtc gcggttgccg tccacccagc
     1261 tactgaccgt gaggacgctg gcgttcagca gcagcgcgtc cgtcgcctgc caggtcgcgt
     1321 tcaggttgcc tttgtgcttc gggcgacgca gcagctcctg atgcagcacc tcgtcggtcg
     1381 cctccgtata ggtgtagtcc agccgcagag tcagcggctt taccggttgg tacgccacga
     1441 aaccctcgac gccgtccgtg ctcgcgcgcc cgacgttggc gtaagtggtt cccgtgacgt
     1501 cggtggtgat caggtcgcga atgcgattgt agaaccaggt ggccccgatc cggaccacgc
     1561 ccccgcccag cccctgttcg attccgagat cgtagcccac gctgctttcg ggcttcagat
     1621 cggggttcgc gaagaagaac ggtggaaagc tctggaacag ctcactcagc gtcggcgcct
     1681 tgaacccgct gccgacgctg gccttgagtt tcgtgcccga tgcagagctc acccaggtcg
     1741 gcgcgatgcg gtaggtcacc ttgctgccaa agcgatcgtt gtcgtcgtag cgcgcgttga
     1801 gggccgagaa ccagtgctcg ccgagttgcg actgcagctc cacgtaagcc gatgcgatgc
     1861 gcatgctggc ggagatcggc gcgctgatct catcccgcgc atcctccaga ccgagcagca
     1921 ccgagtgcgc actcgagact cggatgacgc cctgccagtc gagtttgacg cgctcgccgg
     1981 tgttgagact ttccggagtc tgcggttgca gcgttgccgt gcggttgcgc gtgaaggcga
     2041 cgccgagggt ctgattgagg ccgccgtccg gcgccagcag gtgcgcggtg gcacgcccgt
     2101 agtactcatc ggtgtcactg gcactctgct gcgcggccgg ca
//
LOCUS       DATMRM010000066         3312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1590395, whole genome shotgun sequence.
ACCESSION   DATMRM010000066 DATMRM010000000
VERSION     DATMRM010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590395"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VNZ06_01990"
     CDS             <1..491
                     /locus_tag="VNZ06_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19551.1"
                     /translation="AMSWTLAAVCVSGISLRRQSAVMIGGPLIMGIGLAGIAWRMPSG
                     TVTQLLLPIFLSGFGIGACWSFIAQRVMSCARADDAEVAASSLATVQLYGFAFGGAIA
                     GLIANLIGYSQGLTLETTRLAAFWVPASFVSVTLLAALAAARLLVLGRVGHANPSSVS
                     HS"
     gene            complement(474..1643)
                     /locus_tag="VNZ06_01995"
     CDS             complement(474..1643)
                     /locus_tag="VNZ06_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HWW19552.1"
                     /translation="MPANPAEFVLSDETLQDLAARLHSTRLPPDDAHDWHAGVNPAYL
                     RELLGYWRTEFDWRRQESALNAFRHCRAEIGGSIVHYIHERSRHPNALPVILTHGYPD
                     SFVRFLKLIPWLTDPVAHGGRKEDAFDVVVPSLPGYGYSRGSMKANEIYHVGDLWHEL
                     MSGVLGYKRFGAHGGDWGSLITEQLGRNHSNAVVGIHLTDVPLSHVFHRPRRISSTEA
                     HYLRSISEFEAREGAYAMIQGTRPLTLADSLNDSPAGLAAWLVDKFQRWSDCDGEVEN
                     RFTKDELLTNVTIYWATQSIGTSFLPYFELMHAGASRSLMELAKARLRPPATPAGFAL
                     FPKDISIPPLSWAKRFFHVTRWSQMPRGGHFGAMEEPQLLAEDMRAFFAPLRVTH"
     gene            complement(1624..2844)
                     /locus_tag="VNZ06_02000"
     CDS             complement(1624..2844)
                     /locus_tag="VNZ06_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HWW19553.1"
                     /translation="MEPEPFKLHVERDVLQDLRVRLERTRRVRGIAAPDWKHGVDPSY
                     LDELIAYWLNGFDWQLQEEKLNAQPHYRASVDGARLHFVHARGRGSENLPLLLLHGWP
                     DSFLRYVKLIPILADPRRMGFSLDDAFDVVVPSLPGFPLTGPVAHPAGRSANRHSAQL
                     LWRLMTEVLGYERFAVAGGDGGSMLAQILAIDYPDSVIAIHLTDIGWHATDVNMDWLS
                     RAEAKYVRAAHRRFLADGAYAAVQMSQPRSLAVGLNDSPVGLASWIVDRFHSWSDPRA
                     DFRRAFRKDVLLTNIMLYWTTQTIGSSMLSYYSEAHRPSLTPSDYVDCPVGLALFPFD
                     GAGVPPRSFAERTLNVRRYARMSSGGHFAALEVPGLLVREIFDFLGPLRRWNRIAASA
                     VRRAEVAQYAREPG"
     gene            complement(2915..>3312)
                     /locus_tag="VNZ06_02005"
     CDS             complement(2915..>3312)
                     /locus_tag="VNZ06_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWW19554.1"
                     /translation="IFSGHVVDEAHRPSFLFNPSNDAWFGAWGPAQHLAQAQMRAIEE
                     AIPIIRATPNGISALIGPGGNLIQSVPRHQTGLINAAVPRALPPTIFSRYGLLTSLMF
                     GSALLLIGLILRRARVGNEGLELREAGRT"
BASE COUNT          633 a         1037 c         1045 g          597 t
ORIGIN      
        1 aggcgatgtc gtggacgttg gccgccgtct gcgtctcggg catctcgctg cggcgccagt
       61 ctgcggtgat gattggcggg ccgctcatca tgggaatcgg cctggcaggg atcgcctggc
      121 gcatgccctc gggcaccgtc acacagctac tgctgccgat cttcctttcc gggtttggta
      181 tcggcgcctg ctggtcgttc atcgcgcaac gtgtcatgag ctgtgcgcga gccgatgacg
      241 ctgaagtcgc tgcctcctcg ctcgccacgg tgcagctcta cgggttcgcg ttcggtggcg
      301 cgatcgcagg gctcattgcc aatctgatcg gctactcgca agggttgacg ctcgagacga
      361 cacgactggc ggccttctgg gtgccggcgt cattcgtcag tgtcacgctg ctggcggcgc
      421 tggccgccgc gcggctgctg gtgctggggc gcgtggggca cgcgaatcca agctcagtga
      481 gtcactcgta gcggggcgaa gaaggcacgc atatcctcgg ccagcagttg cggctcttcc
      541 atggcgccga aatgaccgcc gcgcggcatc tgcgaccagc gtgtcacgtg aaagaagcgc
      601 ttcgcccagg aaagcggcgg aatggaaatg tccttcggga agagagcgaa ccccgccggt
      661 gtagcgggtg gtctgagccg tgccttggcc agctccatca gcgagcgcga ggcacccgcg
      721 tgcatcagtt cgaaatacgg caggaacgac gtgccgatcg actgcgtcgc ccagtagatg
      781 gtcacattgg tcaacagctc gtccttggtg aacctatttt cgacttcgcc gtcgcagtcg
      841 ctccagcgct gaaatttatc taccagccag gccgccagtc cggccggtga gtcgttcagg
      901 ctgtctgcca atgtgagcgg acgcgttccc tggatcattg cgtaggcacc ttcgcgcgcc
      961 tcaaactcgc tgatcgagcg cagatagtga gcctcagtgg aactgatgcg acggggtcgg
     1021 tgaaacacgt gtgagagcgg cacatccgtc aggtgaatgc cgaccacggc attcgaatga
     1081 ttgcgcccga gctgctcggt gatcaggctg ccccaatcgc cgccgtgtgc gccgaagcgc
     1141 ttgtaaccaa gcactccgct catgagttcg tgccacagat ctccgacgtg gtagatctca
     1201 ttggccttca tcgagcctct ggaatagccg taacccggca gactcggaac aaccacatcg
     1261 aacgcatcct ccttgcgccc gccgtgcgcg accggatcgg tcaaccatgg aatgagtttc
     1321 aggaagcgta cgaacgaatc agggtagccg tgcgtgagaa tgacgggcag ggcattggga
     1381 tggcgactgc gctcatgaat gtagtgcacg atactgccgc cgatttccgc gcggcagtgt
     1441 cgaaacgcgt tcagcgccga ttcctgcctg cgccaatcaa actcggtgcg ccaataaccc
     1501 agcagttcgc gcaagtaggc cggattcacg ccggcgtgcc aatcgtgcgc gtcgtcgggc
     1561 ggcagacgtg tgctgtgcag acgcgccgcc aggtcctgca gcgtttcatc cgacagcacg
     1621 aactcagccg ggttcgcggg catactgcgc cacctctgct cgccgtaccg cactcgccgc
     1681 gattcggttc cagcgccgaa gcgggcccag gaaatcgaag atctcccgta ccagcaggcc
     1741 cggcacctcg agcgctgcga aatggccgcc gctggacatt cgggcatagc gccgcacgtt
     1801 cagcgtgcgc tccgcgaacg agcgcggcgg aacgccggcg ccgtcaaacg ggaacaacgc
     1861 cagtccgacc gggcagtcga cgtagtcgct cggcgtgagc gaggggcgat gagcttcgga
     1921 gtagtaggac agcatcgacg agccgatcgt ctgtgtcgtc cagtacagca tgatattggt
     1981 cagcagtacg tccttgcgga aagctcgtcg gaaatccgcc cgtggatcag accacgagtg
     2041 gaatcggtcc acgatccagg aagccagacc gaccggagag tcgttcagtc cgactgccag
     2101 cgaacgcggc tgtgacatct gcacggccgc atacgcgcca tcggcgagaa atcgcctgtg
     2161 cgcggcccgt acgtatttcg cttcggctcg ggagagccag tccatattga cgtcggtcgc
     2221 gtgccatccg atgtcggtca gatgaatggc gatcaccgag tccggatagt cgatcgccag
     2281 gatctgcgcc agcatgctgc cgccatcgcc gccggcgacg gcaaagcgct cgtagcccag
     2341 cacctccgtc atcaggcgcc acagcagctg cgcactgtgc cggttggcgg aacgacccgc
     2401 aggatgcgcc acaggcccag tcagcggaaa gccgggcaac gaaggcacca ccacatcgaa
     2461 cgcatcatcg aggctaaagc ccatgcgccg cggatcagcg agtatcggaa tgagtttgac
     2521 gtaacgaagg aaactgtcag gccagccatg aagcagcaac agcggcagat tctcgctgcc
     2581 gcggcccctg gcgtgcacga agtgcagtct ggccccgtcc acgctcgccc ggtagtgagg
     2641 ctgggcgttc agcttttctt cctgcagctg ccagtcgaat ccgttcagcc agtaagcgat
     2701 cagctcatcg aggtatgaag gatcaacgcc gtgcttccag tccggcgctg caataccgcg
     2761 tacacgccgc gttcgctcaa gccggacccg cagatcctgc agcacatcgc gttcgacgtg
     2821 cagcttaaat ggctcgggtt ccaccgagtg attgtctgga acgcgttact gcgcggccaa
     2881 gctggcaatc cgccgattgt gcgtcagtat cgtcctatgt cctgccagct tcccgtagct
     2941 cgagtccctc attgccaact cgcgcgcgac gcagaatcag cccgatcagc agcaatgcgc
     3001 tgccgaacat aagactggtg agcagtccgt atcgggaaaa gatcgtggga ggcagcgcac
     3061 gtggcaccgc cgcgttgatg aggcctgtct ggtgacgcgg caccgactga atcaggttgc
     3121 ccccagggcc gatcagcgcg gaaatgccgt ttggcgtcgc gcgtatgatc gggatcgctt
     3181 cctcgatggc acgcatctgc gcttgtgcca gatgttgcgc aggtccccag gcaccgaacc
     3241 aggcatcgtt ggacggattg aagaggaagg acgggcgatg cgcctcatcg accacatgcc
     3301 ccgagaaaat ga
//
LOCUS       DATMRM010000067         7468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_659836, whole genome shotgun sequence.
ACCESSION   DATMRM010000067 DATMRM010000000
VERSION     DATMRM010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7468
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659836"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2369)
                     /gene="mutS"
                     /locus_tag="VNZ06_02010"
     CDS             complement(<1..2369)
                     /gene="mutS"
                     /locus_tag="VNZ06_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HWW19555.1"
                     /translation="MNDRPSEYAEHTPMMQQYLRIKAQYPQALLLYRMGDFYETFYED
                     ARVAASLLDIALTRRGQSAGAPIPMAGVPVQSLDAYLARLVRRGQSVAICEQTGDPGK
                     TRGPMERQVVRVVTPGTVTDEALLDQRREVLLAALVMDHTHAGLAWLELSSGRFSVLE
                     SREAGAIEAELERLRPAELLLPEDQSRELLTGLRQAPVVRTRPVWHFELASASRVLTD
                     QMGTLGLTGFGVDELPFAIRAAGALLQYVRDTQKSALPHIRAMRVEERGEALILDATT
                     RRNLELDESLSGSEGASLFAVLDHTITAMGSRALRRWLNRPIRAHGVLRERFQAIASL
                     ADSRRYEALREQLRAVGDLERILARVALRTARPRDLVQLRSSLGSIPAVRSTLTQFDS
                     PLLAQLQSRLSEHIVERAVLERAVDPEPPHFLRDGGVIAIGYDSELDELRRIATNTDA
                     FLLELEQRERERSGIASLKLGYNRVSGFYIEVSRRDAERVPPDYLRRQTVKSAERFVT
                     PELKGFEDRVLGARERALTRERELYEDLLGTLIEALPALQASAGALAETDALAALAQR
                     AVTLDWTEPTLLDEPRLVLEAVRHPVVESCINTPFVPNDVALHEGRRMLIITGPNMGG
                     KSTYMRSVALAVVLAHVGSFVPAARAQIGPIDRIFTRIGAADDLAGGRSTFMVEMTET
                     ANILHNATDRSLVLMDEIGRGTSTFDGLSLAYATARHIAEHLRPFTLFATHYFELICL
                     AQELQACANVHLDATEHGDSLVFLHAVKEGPANRSFGLQVAQLAGVPREV"
     gene            complement(2413..3846)
                     /locus_tag="VNZ06_02015"
     CDS             complement(2413..3846)
                     /locus_tag="VNZ06_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWW19556.1"
                     /translation="MAPIFDKLFKGKAAAANPAATPTPEQYEAFLAQATSAAAARAFQ
                     QAIALCDRAIAADPSRAEGYYKRANALKDLGQLDAAITGYNQAIERKPDYAYAYCNRG
                     FVEHRKGCVDAALKSFDRAIELDPDDAFPHYNRAMALQDCQRWNEAIASYDRAIAANP
                     EFADAQFNRAMALLYTGDFASGWPAWEWRWKNAQRLGIGEERHFRQPLWLGDEPLGGK
                     RLLIHSEQGLGDTLQFCRYAALCAQRGATVILEVQPALSELLADLDGVTELIPKGELL
                     PAFDYHCPIMTLPLAFRTTLDTIPAPASYLRSDPTRVAHWQSTLGERNGARVGLVWSG
                     NARNYIDQRRSIRLADWIRHLPAQYQYYQLQTQVRPEDEAALDATECLFSFEDELLDF
                     ANTAALCDCMDLVISVDTSLAHLSGALGRRTWMPLAHTPDWRWMRERPDTPWYPQTRL
                     YRQTQAGDWRPVFERIASDLRKEFPAR"
     gene            3912..4397
                     /locus_tag="VNZ06_02020"
     CDS             3912..4397
                     /locus_tag="VNZ06_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HWW19557.1"
                     /translation="MSFRLYSDDLKEGDFLPSAQVFNGFGHNGANISPHLAWSDPPAG
                     TQSFVVQLYDPDAPTGSGWWHWIVANLPASCRALPRGAGSGQASMPPGVVQARNDYGK
                     SNFGGAAPPPGPPHRYIWTVNALKVDLIDVTPDSSGALVGFMVTMNKIASAQLLTRYG
                     V"
     gene            4401..4901
                     /gene="pncC"
                     /locus_tag="VNZ06_02025"
     CDS             4401..4901
                     /gene="pncC"
                     /locus_tag="VNZ06_02025"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF002975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HWW19558.1"
                     /translation="MDDFNDSVLRSLAERAGELLLARGRRAVTAESCTGGWVAKCLTD
                     IPGSSKWFERGYVTYSNAAKEQEIGVSAQALATFGAVSRPVVEQMALGALKGSGADLC
                     VAITGIAGPDGGTVEKPVGLVWFGLADARAARPIAQRQLFSGDRDRIRRASVATALQM
                     LIEAVH"
     gene            4907..5428
                     /gene="thpR"
                     /locus_tag="VNZ06_02030"
     CDS             4907..5428
                     /gene="thpR"
                     /locus_tag="VNZ06_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HWW19559.1"
                     /translation="MFFAFWPPAGWVQPLMQAAAPVVAQAGGRTMQSSDMHVTLCFLG
                     SVDESRIEALRARVAAVRAGALTLRFDRIEFWREPRVLVATAQPTAAGLALAQSLQQA
                     ASDLGLAPDRKPWRPHMTLARSVLPKRLPPAFQSERPMPSPLRLTVSSFLLAESLIGA
                     QRRYGTLASWPLA"
     gene            5583..6602
                     /gene="recA"
                     /locus_tag="VNZ06_02035"
     CDS             5583..6602
                     /gene="recA"
                     /locus_tag="VNZ06_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004812670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HWW19560.1"
                     /translation="MEDSNRKKALVAALGQIEKQFGKGSVMRLGDASAVYDVEAVSTG
                     SLGLDIALGIGGLPRGRVVEIYGPESSGKTTLTLQVVAEVQRQGGTAAFVDAEHALDP
                     SYAGKLGVNVQDLLVSQPDTGEQALEITDMLVRSGAVDVVVIDSVAALTPKAEIEGEM
                     GEMQMGLHARLMSQALRKLTGNIKRSNTIVIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAIKNGEEVVGNQTRVKVVKNKVAPPFREAEFEIMYGSGISREGEII
                     DLGSTHGIIDKSGAWYSYNGERIGQGKDNTRLYLQQHKDIASEIESKVRLKLLPPKSV
                     EEKTA"
     gene            6646..7080
                     /locus_tag="VNZ06_02040"
     CDS             6646..7080
                     /locus_tag="VNZ06_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HWW19561.1"
                     /translation="MAAAGSLLARRDYCSSELAARLVSRGFAPDTVQATLADLHARHY
                     LDDARYARQFVDTHARRGHGPVLIRHELGERGLSPQLAEQSLADFGDWSALAREVRAR
                     RFGAQPPRSRSDKTRQARFLQYRGFSDDDIRGALGFDVTSDC"
     gene            7108..>7468
                     /locus_tag="VNZ06_02045"
     CDS             7108..>7468
                     /locus_tag="VNZ06_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HWW19562.1"
                     /translation="MRAAQVRRMFLEYFRQNAHTVVPSSSLVPGNDPTLLFTNAGMVQ
                     FKDLFLGKERRDYVRATSSQRCVRAGGKHNDLENVGYTGRHLTFFEMLGNFSFGDYFK
                     REAIRFHWELVTSEQWFG"
BASE COUNT         1296 a         2335 c         2467 g         1370 t
ORIGIN      
        1 acctcacgcg gcacgccggc taattgcgcc acctgcagcc caaagctgcg attagccggt
       61 ccctccttca ccgcgtgcag gaacacgagc gagtcgccgt gctcggtggc atccaggtgc
      121 acgttggcgc aggcttgcag ctcctgcgcc agacaaatga gctcgaagta gtgagtcgcg
      181 aacaacgtga acggacgcag gtgctcggcg atgtgtcggg ctgtggcgta ggcgagcgac
      241 agcccgtcga aagtgctggt gccgcggccg atttcatcca tcagtaccag actgcgatcc
      301 gtggcgttgt gcagaatgtt ggcggtttcg gtcatctcca ccatgaaggt tgatcggcca
      361 ccggccagat catccgcggc cccgatgcgc gtgaagatgc gatcgatcgg accgatctgg
      421 gcccgagcgg cgggcacgaa gctgccgaca tgggctagca cgaccgcgag tgcgaccgag
      481 cgcatgtaag tggacttgcc gcccatgttc ggcccggtga tgatcagcat ccgtcgtcct
      541 tcatgcagcg ccacatcgtt cggaacaaac ggggtattga tgcagctttc caccactggg
      601 tggcgcaccg cctcaagcac cagacgtggc tcgtcgagca gcgtcggttc ggtccagtcg
      661 agcgttaccg cgcgctgtgc cagtgccgcc agcgcatccg tctcggccag cgcgcccgcg
      721 ctggcctgaa gcgcaggtaa cgcctcgatc agtgtgccga gcaagtcttc atacagctcg
      781 cgttcgcggg tcagcgcacg ttcgcgcgcg ccgagcactc tgtcttcgaa gcccttgagt
      841 tcgggagtca cgaaacgctc ggcgctcttg acggtctgtc ggcgcaagta gtccggaggt
      901 acccgctccg catcgcgtcg tgagacttcg atatagaacc cggagactcg gttatagccg
      961 agcttcagtg aggcgatgcc actgcgctcg cgctcacgct gctcgagttc gagcaggaac
     1021 gcgtccgtgt tcgtggcgat gcgccgcagt tcatcaagct cagagtcgta gccgatggcg
     1081 atgacgccgc cgtcgcgcag gaagtgaggc ggctccggat cgaccgcgcg ctcgagcaca
     1141 gcgcgctcga cgatgtgctc ggaaagccgc gactgcagtt gcgcgagcaa gggcgagtcg
     1201 aactgcgtca gcgtgctgcg aacggcagga atgctgccaa gggagctgcg cagctgcacc
     1261 agatcacgcg ggcgcgcggt acgcagtgcg acgcgcgcca ggatgcgctc gagatctccg
     1321 accgcccgca gctgctcgcg caacgcctcg tagcggcgcg aatcagcgag cgaggcgatg
     1381 gcctggaagc gttcgcgcag tacgccgtgt gcgcggatcg gacgattgag ccagcgtcgc
     1441 agcgctctcg agcccatcgc cgtgatggtg tggtcgagca ccgcgaacaa ggatgcgcct
     1501 tcgctgcccg acagactctc gtccagttcc aggttgcgcc gcgtggtggc atccaggatc
     1561 agtgcttcac cgcgctcctc gacgcgcatc gcgcggatgt gcggcaacgc cgatttctgg
     1621 gtgtcgcgca cgtactgcag cagcgcgccg gcggcgcgga tcgcgaacgg cagctcatcg
     1681 accccaaacc cggtgagccc gagtgtgccc atctggtcgg tgagcacacg cgacgcactc
     1741 gccagctcga aatgccacac aggccgtgta cgaaccacgg gcgcctgtcg cagacccgtc
     1801 agcagttcgc gcgactggtc ctccggcagc agcaattccg ctgggcgcaa gcgctccagc
     1861 tcggcctcga tcgcacccgc ctcgcgcgat tcgagcacgc tgaagcgtcc ggacgagagt
     1921 tccagccacg caaggcccgc gtgcgtgtga tccatcacca gcgctgccag caggacttcg
     1981 cggcgttgat cgagcagcgc ctcgtcggtc acagtgcccg gcgtgaccac ccgcaccacc
     2041 tggcgctcca tcggaccgcg ggtcttgccc ggatcgcccg tttgctcgca gatcgccacc
     2101 gactgacccc gccgcaccag gcgtgcaaga taggcatcca ggctttgcac cggcactccg
     2161 gccattggaa tcggcgcgcc ggcagactgc ccgcgacgcg tcagtgcgat gtcgagcaga
     2221 ctcgctgcga ctcgggcatc ctcgtagaag gtctcgtaga aatcgcccat ccgatacagc
     2281 agcagcgctt gcgggtactg cgccttgatg cgcaggtact gctgcatcat cggggtatgt
     2341 tcggcgtatt cggaagggcg gtcgttcacg acgacaccgt ctgcggcgca ggcatgcgtt
     2401 gtccgcgccc gttcagcggg cgggaaactc tttgcgcaga tcggacgcga tgcgctcgaa
     2461 tacaggcctc cagtcgccgg cctgagtctg acgatagagt cgggtctgcg gataccaggg
     2521 cgtgtccggc cgctcgcgca tccatcgcca gtcgggcgtg tgcgccagcg gcatccaggt
     2581 tcgacggccg agggctccgc tcagatgcgc cagactggta tccacgctga tcaccaggtc
     2641 catgcaatcg catagcgccg ccgtgttggc aaaatccagc agctcatctt cgaacgagaa
     2701 taggcactcg gtcgcgtcga gcgcggcttc atcctctggc cgcacctggg tctgcagctg
     2761 atagtactga tattgcgcag gcagatggcg gatccagtca gccagccgaa tactgcgccg
     2821 ctgatcgatg tagttcctgg cattgccgct ccacaccagg ccgacacgcg cgccgttgcg
     2881 ctcgcccagt gtgctttgcc agtgcgcgac gcgggtagga tcactgcgca gatagcttgc
     2941 cggcgcagga atcgtgtcca atgtcgtccg gaacgccagg ggcagggtca tgatcggaca
     3001 gtgatagtcg aacgcaggca gcagctcacc cttcggtatc agttcagtga ccccgtccag
     3061 atcagccagc agctcagaca gcgcgggctg gacctcgaga atcaccgtcg cgccgcgttg
     3121 ggcgcacagt gccgcgtagc ggcaaaactg cagtgtgtct cccagcccct gttcgctgtg
     3181 aatcagcaac cgcttgccgc ctaagggttc atcgccgagc cagagcggct gcctgaagtg
     3241 ccgctcttca ccaatgccca ggcgttgcgc gttcttccaa cgccactccc acgccggcca
     3301 gccgctggcg aaatctccgg tgtacagcag cgccatcgca cgattgaact gtgcgtcggc
     3361 gaattccgga ttggcggcga tggcgcggtc atagctggcg atggcctcat tccatcgctg
     3421 gcagtcctgc agcgccatgg cccggttgta gtgcgggaag gcatcgtccg gatcgagctc
     3481 gatcgcccgg tcgaaactct tgagggcggc gtccacgcaa cccttgcggt gttccacgaa
     3541 gccccggttg caataggcgt aggcgtagtc gggcttacgt tcgatcgcct ggttgtagcc
     3601 ggtgatcgcc gcatccagct gccccagatc cttcagcgca ttggcgcgct tgtaatagcc
     3661 ttctgcacgc gaggggtcgg ccgcgatcgc ccggtcgcac agcgcaatgg cctgctggaa
     3721 ggcgcgcgct gctgcggcgc tcgtggcctg cgccagaaac gcttcgtact gctcgggcgt
     3781 cggtgtggcg gccggattgg ccgcggctgc ctttcccttg aacagcttgt cgaatatcgg
     3841 ggccatggat gatggcgcag tctacagtct caggcgctaa gatcgcacct tcacgcctgg
     3901 aggtgccgcg catgagtttt cgactgtaca gcgacgatct gaaggagggt gatttcctgc
     3961 cctcggcgca agtcttcaac ggttttggcc acaatggggc caatatttcc ccgcatctcg
     4021 cctggagcga cccgcccgcc ggcacgcaga gtttcgtcgt tcagctctat gatccggacg
     4081 ctccgaccgg ttccgggtgg tggcactgga ttgtcgccaa cctcccggct tcctgtcgcg
     4141 cgttgccgcg cggcgccggc agcggccagg cctcgatgcc accgggcgtc gtacaggcca
     4201 gaaatgacta cggcaaatca aactttggcg gcgcggcgcc tccgcccggg ccaccgcatc
     4261 gctacatctg gacggtcaat gcgctgaagg tggacctgat cgatgtgaca cccgacagca
     4321 gtggcgcgct ggtgggattc atggtgacga tgaacaagat tgccagtgcg cagcttctga
     4381 ctcgctacgg cgtgtagcgg ttggacgatt tcaacgacag cgtgttgcgg tcgttggccg
     4441 agcgcgccgg cgagctgctg ctggcgcggg gacgtcgtgc ggtgacggcc gaatcgtgca
     4501 ccggaggctg ggtcgccaag tgtctgacgg acattccggg cagctcgaag tggttcgagc
     4561 gcggctacgt cacgtattcc aatgcggcca aggagcagga gatcggagtc agtgcccaag
     4621 cgctcgcgac gttcggggcg gtcagccgcc cggtggtcga gcagatggcg ctgggcgctc
     4681 tcaagggcag cggcgcggac ctgtgtgtgg cgatcaccgg aattgccggg ccggacgggg
     4741 ggacggtgga gaaacccgtg ggtctggttt ggtttgggct ggcggatgcc cgggccgcgc
     4801 ggccgatcgc gcaacgacag ctgttcagcg gcgatcgtga caggattcgt cgggcgtcgg
     4861 ttgccacggc tctgcagatg ttgatcgagg cggtgcactg aaacggctgt tttttgcgtt
     4921 ctggccaccg gcagggtggg ttcagccgct gatgcaggcc gctgcgcccg tcgtggcaca
     4981 ggccggcggt cgcacgatgc agtcctcgga catgcacgtg accctgtgtt tcctcggttc
     5041 ggtggatgag tcgcggatcg aagcgctgcg cgcgcgcgtc gccgccgtgc gcgctggcgc
     5101 cctgacgctc cggttcgacc gtatcgagtt ctggcgtgag ccgcgggtgc tggtcgccac
     5161 cgcgcagccc actgccgcgg gtcttgctct ggcacagtcg ctgcagcagg cagcgtcgga
     5221 tctggggctg gcgcccgatc gtaagccttg gcggccgcac atgaccctgg cgcgatccgt
     5281 actgcccaag cggttgcccc cggcatttca aagcgagcgc ccgatgccca gtcccctgag
     5341 attgaccgtc agcagtttct tgctcgccga aagcctgatc ggcgcgcagc gtcgctacgg
     5401 gacgcttgcc agctggcctc tggcttgagc gcatttttct ggtggtgggg tgcaggcgag
     5461 cgccggtctg aggctggagc gtttttttct aatgttccca ccgatttgcg cgctgcgcgg
     5521 cgcgcccgtt gtgggataat tgcgaatcac tcgtctgagg accgtcgcgc atggaacaac
     5581 gcatggaaga cagcaatcgc aagaaagcac tcgtcgccgc gctcgggcag atcgagaaac
     5641 aattcggcaa gggatcggtc atgcgtctgg gtgatgcctc cgcagtctat gacgtggaag
     5701 ccgtgtcaac cggctcgctg ggtctggata tcgcactcgg gatcggtggc ctgccgcgcg
     5761 gtcgcgtggt cgaaatctat ggtccggagt cctcaggcaa gaccaccctg acattgcagg
     5821 ttgttgccga ggtgcagcgg caaggcggca cagccgcatt tgtggacgct gagcacgcgc
     5881 tcgatccgag ctacgccggc aagctcggtg tcaatgtgca ggatctgctg gtctcgcagc
     5941 cggacaccgg cgagcaggca ctggagatca cggacatgct ggtgcgctcg ggcgcggtgg
     6001 acgtggtcgt gatcgactcg gttgcagcgc tgacgcccaa agcggagatc gaaggcgaga
     6061 tgggcgaaat gcagatgggc ctgcacgccc gattgatgtc gcaggcgctg cgcaagctga
     6121 ccggcaacat caagcgctcc aacaccatcg tgatcttcat caaccagatc cgcatgaaga
     6181 tcggtgtgat gttcggcaac cccgagacca ccaccggtgg caacgcgctg aaattctatg
     6241 cctcggtgcg actggatatt cgccgcattg gcgccatcaa gaatggcgaa gaagtggtcg
     6301 gcaatcagac gcgcgtgaaa gtggtcaaga acaaggtcgc tccgccgttt cgtgaggctg
     6361 agttcgaaat catgtacggc tcaggcatct cgcgcgaggg cgagatcatc gatctcggtt
     6421 ccacgcatgg catcatcgac aagtccggcg cctggtacag ctacaacggt gagcgcatcg
     6481 gccagggtaa ggacaacacg cgactgtatt tgcagcagca caaagacatt gcctcggaga
     6541 tcgagagtaa ggtccgtctg aagttgctgc cgccgaagag tgttgaggaa aagacagcct
     6601 agcaggcgag tctcgagcga cgagtccggc gacccggcgg ccgcgttggc ggctgccggg
     6661 tcgcttctgg ccaggcgcga ctactgctca tccgaactgg cggcgcggct ggtgtcgcgc
     6721 ggctttgcgc ctgataccgt acaggcgacc ctcgcagact tacacgctcg gcactacctc
     6781 gacgacgcgc gctacgcgcg gcagttcgtt gacacgcacg cgcggcgcgg ccacggtccg
     6841 gtgctcatcc ggcatgagtt gggtgaacgg ggcctgagcc cgcagctcgc cgaacaatca
     6901 ctggctgatt tcggcgattg gtcagcgctg gcgcgcgagg tacgcgcccg gcggttcggc
     6961 gcccaaccac cccgcagccg gtcggataag acgcgccagg cgcgcttttt gcagtatcgt
     7021 ggtttttccg acgatgatat tcgtggcgcc cttgggtttg atgttacgag tgattgttaa
     7081 gttatgacag ataaatcgac ctcaacaatg cgcgctgcgc aagtgcggcg catgtttctg
     7141 gagtacttcc ggcagaacgc tcacaccgtg gtgccgtcct cctcgctcgt gccaggcaac
     7201 gatccgacgc tgttgttcac caacgccggc atggtgcagt tcaaggatct gtttctgggc
     7261 aaggagcggc gcgactacgt gcgcgccacg tcgagccagc gctgcgtgcg agccggcggc
     7321 aagcacaacg atctggagaa tgtcgggtac acggggcgcc atctgacctt cttcgagatg
     7381 ctcgggaatt tcagctttgg tgactacttc aagcgtgaag caatccgctt tcactgggag
     7441 ctggtcacga gcgaacaatg gttcggtg
//
LOCUS       DATMRM010000068         6698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2513029, whole genome shotgun sequence.
ACCESSION   DATMRM010000068 DATMRM010000000
VERSION     DATMRM010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2513029"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VNZ06_02050"
     CDS             complement(<1..608)
                     /locus_tag="VNZ06_02050"
                     /inference="COORDINATES: protein motif:HMM:NF013011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HWW19563.1"
                     /translation="MSRSRTLAQRWLASLSLGLLPLLLPLAVHAAASVAPAAGIPAFA
                     VQSTAGGGQTYTLTIQLLALMTAITLLPAVLLMMTSFTRIIIVLSILRQALGAGQSPP
                     NQVLVGLALFLTLFVMAPVVEKIRTDAVTPYMSGSIETTEALSRGVEPLKTFMLEQTR
                     ESDIAAFVRMSDGKGYATPKDVPLTILAPAFVTSELKTAFQIG"
     gene            complement(605..1090)
                     /gene="fliO"
                     /locus_tag="VNZ06_02055"
     CDS             complement(605..1090)
                     /gene="fliO"
                     /locus_tag="VNZ06_02055"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HWW19564.1"
                     /translation="MNATVRRPIARPGRIACAWAALACGVMQLSTSAWAAAFAAPEQV
                     APSSSGGGLLRVVIALLVVLGAVVAAGRFARRMRGLGGGTHSALEVVGQLPVGPRERA
                     VLIRVGERQLLLGVAPGNVRTLHVFDDMPVARASAADSAGPAAAARPTFKSLLLKSLG
                     K"
     gene            1412..3367
                     /locus_tag="VNZ06_02060"
     CDS             1412..3367
                     /locus_tag="VNZ06_02060"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HWW19565.1"
                     /translation="MKTTTVRIAACTAVAAVLAACGGGSSDSGMTSNPTGSVQLGTMP
                     VVISDASSDDWALIGVRVLSIALVPSGGGSNVTVYTAGSAAPYLNLEQLDNLGEILGN
                     VSVPVGSYTGAVLTIGANPGDVLLTVAPNPESGFAGNPGEVISSDNIQIQHTRGSAGS
                     LTATINVNFSNSLVIDSAGTPNPALDLEFDLSHPAFIVGHEPPGAGTTLWAVNFQAPV
                     HRHPIADLTRLVLRHAYGNVMSVASDGSSITISKQLPAFPIATPETAVATSQQLMILA
                     DSTNGTLFYDVDAKTSTSITNFSSETSLAGKFVRVAARYQQDGTLVATRIWASGSFAN
                     VWLSPEGHVVNVNRNTDVITVTNEQGVGIPLQVNANTQFFFRQPWSGIADATPIGTGT
                     GFLSSQNLVRGFKVHASVVDPLARPLVAQSIDIEAAAYSGAISQSNSTGFTYTHDYLR
                     AIDDYTFTLPYLSSSSTNPYDPSGSAITGFEYWNFAFPTLVTSGSAVTNDFVQATDGT
                     VSFGGGAAPITAYGTSSALWNDPASPNAWSAPWTILLPVPLPLASVATGVASDSFTVS
                     VPEGTQSVTVNVNSNPGSATLVYQVDRTGGILTISPLDIGTSSGLNTFTTALSAGTPV
                     KVYGIPQADGTLNAYVVTYFTGMMPML"
     gene            complement(3473..4345)
                     /locus_tag="VNZ06_02065"
     CDS             complement(3473..4345)
                     /locus_tag="VNZ06_02065"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HWW19566.1"
                     /translation="MTVPVKQAAGSGDAKETPFQFLASLAKELSGGRVELPSFPDAAA
                     RTQQVLSDENVTSERIARVVGSDAGLAGRVLTMANSTLLHRGGAPVTDLKVAVARIGH
                     DNIRAAALAYANAQLRRAPDLAHIRSQLETCWQDGVRVAALTHAMAKETRGIRSDEAL
                     LAGLLHNIGKIYIIARSPKQGAMQVDDPILRDWYPGIGTALIENWKLPDDIAKAVGGQ
                     LEIDRTHEEPADLQDLLIVAVAVAEQMKANNADDSALARLPSATALGLSDSALVRIML
                     ESQAEMEMLQAALG"
     gene            complement(4361..4708)
                     /gene="fliN"
                     /locus_tag="VNZ06_02070"
     CDS             complement(4361..4708)
                     /gene="fliN"
                     /locus_tag="VNZ06_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HWW19567.1"
                     /translation="MSTTSNAAEVQPAELGTLKPEAPEPGTNRELNLDVVLDIPVTLS
                     IEVGRSRLSIRNLLQLNQGSVVELDRATGEALDVYVNGTLIAHGEVVVVNEKFGIRLT
                     DVISPAERIRKLK"
     gene            complement(4705..5721)
                     /gene="fliM"
                     /locus_tag="VNZ06_02075"
     CDS             complement(4705..5721)
                     /gene="fliM"
                     /locus_tag="VNZ06_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003184019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HWW19568.1"
                     /translation="MTAEVLNQDEIDALLKGVDTGAVPTATDEGPPNAGEVRPFDLAT
                     QTRLVRGRMPTLEMINDRFARLFRIGMFSMLRRTPGIGVAPVAIQKFSEYTQNLQVPS
                     SLNLIRIAPLRGTALMVLDAKLVFAIVDTFFGGTGRYAKIEGREFTATEGRVIQMVLK
                     QAFEDMVQAWNIIANINIEYTSSEINPHFATIVSPSELVVVSSFKIDLEAGGGDLHLT
                     LPYSMLEPIRDVLDSGMQSDRMERDERWTQTLREEIEEAEIEITPVLGRASLTVGRLV
                     DLKPGDVIPCDFDGNITLLAEGVPVARGSYGASRGQQAVKVTQRVTRRKTPVLSNVSV
                     GGKI"
     gene            complement(5725..6312)
                     /locus_tag="VNZ06_02080"
     CDS             complement(5725..6312)
                     /locus_tag="VNZ06_02080"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HWW19569.1"
                     /translation="MSAKAPAAEAKDKDKGKEKGKEAAAPSGKKKSQLPLIIIAAVVV
                     LGGGGAGAWFAFKPKPAAAAAAGADTAAADKAKAEAPEKPSSKKGPPSFYKFDPAFVV
                     NFGGEGSARYLQVTVEAMSRDAAILDELKTDEPAVRNDLVMLFSSQDNATLMSSEGKE
                     KLRAATLDAIRKVLNSEGAKGSKIEAVYFTSFVIQ"
     gene            complement(6427..>6698)
                     /locus_tag="VNZ06_02085"
     CDS             complement(6427..>6698)
                     /locus_tag="VNZ06_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HWW19570.1"
                     /translation="IDMQSSQVNVSFSAPHPDTRSALEQSVPQLRAILANGGLTLGQA
                     TVQQEARPGSHYTPGASRGSSDSAQSVDSVSISPTHGLGLIDEYV"
BASE COUNT         1290 a         2170 c         1982 g         1256 t
ORIGIN      
        1 ccgatctgga acgcggtctt cagctcgctg gtcacgaatg ctggcgcaag aatcgtcagc
       61 ggcacgtctt tcggggtggc gtagcccttg ccgtccgaca tgcgcacgaa tgcggcaata
      121 tcgctttcgc gcgtttgttc aagcatgaat gtcttcagag gttcgacgcc acgcgacagt
      181 gcttccgtgg tctcgatgct gccggacata tagggcgtca cggcgtcggt tcggatcttc
      241 tccaccaccg gggccatcac gaacagcgtt agaaacagcg ccaatccgac cagcacctgg
      301 tttggcggcg actgtccggc acccaatgcc tgccgcagaa tcgagagcac gatgatgatt
      361 cgcgtgaatg aagtcatcat cagcaacact gccggcagca gcgtgatcgc ggtcatcagt
      421 gccagcagct ggatggtcag cgtataggtc tgtccgccac ccgctgtgct ctgcacggcg
      481 aaagcgggaa tccccgccgc aggcgccacc gaggctgctg catgcacggc caatggcagc
      541 agcagcggca acaagcctag cgacaggctc gcgagccagc gctgcgcaag tgttcgagaa
      601 cgactcattt gccgagactc ttcagcagta aggatttgaa ggtcgggcgc gccgcagcgg
      661 cgggcccggc cgaatccgcg gcagacgcgc gcgccaccgg catgtcgtcg aacacatgca
      721 gggtacgcac attccctggc gcgacgccca gcaatagctg acgctcgccg acgcgaatca
      781 agaccgcgcg ctcgcgcggg cccaccggca gttgccccac cacctcgagc gcggaatggg
      841 tgccaccgcc caaaccacgc atgcgtcggg cgaaccgacc ggcggcgacg accgcaccga
      901 gcaccaccag cagtgcgatc actacccgca gcagaccacc gcctgaactg gagggagcga
      961 cctgctcggg cgcagcaaac gcggctgccc aagcgctggt ggacagctgc atcaccccac
     1021 aggccagcgc agcccaggcg caagcgatgc gcccgggacg cgcaatcgga cgtcgaacgg
     1081 tggcgttcaa gatctaccag ctcgctttag gttcacgacc agcctaaagg agcaacccgc
     1141 atgccacgca cgtagggatg cgtctaagtg gttctgtgtg tttgatttgc tgcaggtttc
     1201 ccgctgtgaa gcagttcaca tcgcgcctgt ctgccggata tttggcgctg agctgcggcc
     1261 gtcgagtcgc cctgtctgcg atgcccgacg gccaacggtc actgtcaacc gatgcagatt
     1321 tgattcgctc tgcatccaac gaccaaggac agcaggacgt ccgcagtcga aggcgaaccg
     1381 cgttatggaa agtatcaaac aaggtgaggt tatgaagacg acaactgtgc gaatcgcagc
     1441 ctgcacagcc gtagcggccg tgctcgccgc gtgcggcggt ggaagctccg actccggcat
     1501 gacatcgaat ccgacgggct cggtgcagct cggcacgatg ccggtggtga tcagtgacgc
     1561 ctcctcggat gactgggcgc tgatcggtgt gagggtactg agcatcgcgc tggtaccgtc
     1621 cggtggcggc agcaacgtga cggtctatac cgcaggctcg gccgcgccgt acctcaatct
     1681 cgaacagctc gacaatctgg gagaaatact cggcaacgtg tcagtgccag tgggtagcta
     1741 cacgggcgcc gtgctgacca tcggcgcgaa tccgggcgac gtgttgctga ccgtggcgcc
     1801 gaatccggaa agcggattcg ccggcaatcc cggagaggtc atcagctcgg acaacatcca
     1861 gattcagcac acgcggggca gcgccggcag cctgaccgcg acgatcaacg tcaacttcag
     1921 caactcgctg gtcatcgact ccgcgggcac acccaatccg gcactcgatc tggaattcga
     1981 tctgtcgcat ccggcattca tcgtaggaca tgagcctccg ggcgcaggca ccacgctgtg
     2041 ggcggtgaac ttccaggctc cggtgcaccg gcatccgatc gccgatctga cgcgcctggt
     2101 actgcgacac gcctatggca atgtgatgag cgtcgcgagc gacggctcgt ccatcacgat
     2161 cagcaagcag ctgccggcat tcccgatcgc gaccccggaa accgcggtgg cgacctcaca
     2221 gcagctgatg attctggccg atagcacgaa cggcacactg ttctatgacg tggatgccaa
     2281 gaccagcacc tcgatcacca acttttcgag tgagaccagt ctcgccggca agttcgtgcg
     2341 cgttgccgca cgttaccagc aggacggcac gctggtggcc acgcgcatct gggcgagcgg
     2401 cagcttcgcg aacgtatggc tgagtcccga aggccacgtg gtcaacgtca acaggaacac
     2461 cgacgtcatc accgtgacga acgagcaagg cgtaggtata ccgctgcagg tcaatgccaa
     2521 cacgcagttc tttttccgcc agccctggtc gggcattgcg gatgcgaccc cgatcggaac
     2581 cggcaccggc tttctgagca gtcagaacct ggtgcgcggc ttcaaggtac atgcgagtgt
     2641 ggtggatccg ctggcgaggc cgctggtggc gcagagcatc gacatcgaag cggccgctta
     2701 ctcgggcgcc atttcgcagt ccaattccac cgggttcacc tatacccacg actacctgcg
     2761 cgcgatcgat gactacacat tcacccttcc gtatctgtcc agcagcagca ccaatcccta
     2821 cgatccgagc ggtagcgcga tcacgggctt cgagtactgg aacttcgcct tcccgaccct
     2881 ggttacctca ggcagcgcgg tgaccaacga cttcgtgcag gccaccgatg gcaccgtcag
     2941 cttcggcggc ggggcagcac cgatcactgc ctatggcacc agctcggcgc tgtggaatga
     3001 cccggcaagc cccaatgcct ggagcgcgcc gtggaccatt ctgctcccgg taccgctgcc
     3061 gctcgcgagc gtcgccaccg gcgtcgcgag tgacagcttc acggtcagcg taccggaggg
     3121 cacgcaatcg gtcaccgtca acgtcaattc gaatccaggc tcggcgacgc tggtctatca
     3181 ggtcgatcgc accggcggca tcctcacgat cagcccgctc gacatcggga ccagcagcgg
     3241 cctgaacacg tttaccaccg cactgagcgc tggcacgccg gtcaaggtgt atggcatccc
     3301 gcaggcggac ggcacgttga acgcgtatgt cgtcacctac ttcaccggca tgatgccgat
     3361 gctgtaagcc acagtcagaa cggcacgcca gaagaaacgg caccagaaga tagggcccgc
     3421 gagggcccca atcttttcag caacgatccg gcagcggtct aagcgagcgc tatcaaccca
     3481 atgccgcctg cagcatctcc atttcggctt gcgactcgag catgatgcga accagcgccg
     3541 agtcggacaa tcccagcgcc gtggccgacg gcaaccgcgc cagggcggaa tcatccgcat
     3601 tgttagcctt catctgctcc gcgactgcca ccgcgacgat cagcagatcc tggagatcgg
     3661 ccggctcctc gtgcgtgcga tcgatttcaa gctggccgcc cactgctttg gcaatatcgt
     3721 ccggcagctt ccagttctcg atcaacgccg tgccaatgcc tggataccag tcgcgcagaa
     3781 tcggatcgtc tacctgcatc gctccctgct tgggcgagcg cgcgatgatg tagatcttgc
     3841 cgatgttatg cagcaggccg gccaacagcg cctcgtccga gcgaatgccg cgggtctctt
     3901 tggccatcgc atgggtgagg gccgcgaccc gcacaccgtc ctgccagcag gtctccagct
     3961 gcgaacggat atgcgccaga tcgggtgccc ggcgcaactg cgcgttggcg taggccagcg
     4021 cggccgcgcg gatattgtca tgaccaatcc gggccaccgc gaccttcaga tcagtcaccg
     4081 gtgcgccgcc acgatgcagc aacgttgaat tcgccatcgt gagcactcgc ccggccagac
     4141 cggcatccga gcccacgacc cgtgcaatgc gttcgctggt gacattctca tcactgagca
     4201 cctgctgcgt gcgggccgcg gcgtccggaa aggacggtag ctccacccgg ccgcctgaca
     4261 actccttcgc caatgaggcc aggaattgga atggcgtctc tttggcgtcg cctgacccag
     4321 cagcttgctt gaccggcact gtcacgattg cactctccag ctatttcaac ttgcgaatcc
     4381 gttcggccgg gctgataacg tcggtcaaac ggattccgaa tttctcgttc accactacca
     4441 cttcaccatg cgcgatcagc gtcccgttga cgtagacgtc aagcgcttca ccggtggccc
     4501 gatcaagctc cacaaccgag ccctgattga gctgcaacag gtttcgaata ctgaggcggc
     4561 tgcgtccaac ctcgatcgat agtgtgactg gaatgtccag caccacatcg agattcagct
     4621 cacggttcgt gcccggctca ggcgcctcgg gcttcaaagt tcccagttcc gcgggttgaa
     4681 cctcagccgc gttcgatgtc gtactcatat tttcccacct accgagacat tacttaacac
     4741 cggcgtcttg cgccgggtta ctcgttgcgt gactttgact gcctgctggc cgcgcgaggc
     4801 gccgtagctg ccacgggcca ctggcacgcc ctctgcaagc agcgtgatgt tgccgtcaaa
     4861 atcacacgga atgacatcgc cgggtttcag atccaccagc cgtccaacgg tcagcgacgc
     4921 gcgaccgagt accggcgtga tctcgatttc cgcttcctcg atctcttcgc gcagcgtctg
     4981 cgtccagcgc tcgtcgcgtt ccatgcgatc gctctgcatg cccgaatcga gcacatcccg
     5041 gatcggctcg agcatcgagt aaggcagggt cagatgcagg tcgccaccac cggcctcgag
     5101 gtcgatcttg aaggagctga ccacgacgag ttccgaagga ctgacgatgg tcgcaaagtg
     5161 cgggttgatc tcggaactgg tgtactcgat gttgatgttg gcaatgatgt tccaggcctg
     5221 gaccatgtcc tcaaaggcct gcttcaacac catctgaatg actcggcctt cggtggcagt
     5281 gaattcgcgt ccctcgatct tggcgtaacg accggtgccg ccaaagaacg tatccacgat
     5341 cgcgaatacc agcttcgcgt cgagcaccat cagcgcggtc ccgcgcagcg gcgcaatacg
     5401 gatcagattc agactcgagg gtacctgcag attctgcgtg tactcgctga acttctggat
     5461 cgccaccggc gcaacgccga tccctggcgt acgtcgcagc atgctgaaca tgccgatgcg
     5521 aaacaggcgc gcgaagcggt cgttgatcat ctccagggtc ggcatacgcc cgcgcaccag
     5581 gcgcgtctgc gtcgccagat cgaacggccg cacttcacct gcattgggcg ggccttcatc
     5641 ggtcgcggtc ggtaccgcgc ccgtgtctac gcccttgagc agcgcgtcga tctcatcctg
     5701 attgaggact tcagcggtca tgcgttactg gatcacgaag ctggtgaagt acaccgcctc
     5761 gatcttgctg cccttggcac cttcagaatt cagcaccttg cggatcgcat cgagtgtcgc
     5821 ggcgcgcagt ttttccttgc cttcggagct catcagcgtc gcgttgtcct ggctggagaa
     5881 caacatcacc agatcgttgc gcaccgccgg ttcatcggtc ttcaactcgt cgagtatcgc
     5941 ggcatcgcgc gacatggctt caacggtgac ctgcagataa cgcgcactgc cctcgccgcc
     6001 aaagttgacg acgaacgccg gatcaaattt atagaagctc ggaggccctt tcttggagct
     6061 gggtttttcg ggcgcttcgg ccttggcctt gtcggccgct gcagtgtcgg ctcctgcggc
     6121 ggccgcggcc gccggcttgg gcttgaaagc gaaccacgcg cctgcgccac cgccgccaag
     6181 cacaacgact gcggcaatga tgatcagagg tagctggctc ttcttcttgc cagaaggagc
     6241 ggctgcctct ttacccttct ctttcccctt atccttgtct ttcgcctcag cggcgggtgc
     6301 cttcgctgac atgatgatgt gccggttacc tgggttgagt tgagtgcgcc cgatctatag
     6361 agcaagcgtt atgccagcat cagtttgcgc aaaacgacgg aaaactgtcg cactgccagc
     6421 gcacattcac acgtattcgt cgatcaatcc gagaccgtgt gtcggcgaga ttgagacgga
     6481 gtcgacactt tgcgcgctgt ctgaggagcc gcgcgacgca cccggcgtgt agtgtgagcc
     6541 cggtctcgcc tcctgttgca cggttgcctg gccgagtgtc aatccaccgt tggccagtat
     6601 cgcgcgcagt tgcggcacgc tctgttccaa ggcgctgcgc gtatcggggt gtggtgcact
     6661 gaagctgacg ttgacctgcg aagattgcat gtcgatgc
//
LOCUS       DATMRM010000069         4733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1277169, whole genome shotgun sequence.
ACCESSION   DATMRM010000069 DATMRM010000000
VERSION     DATMRM010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277169"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VNZ06_02090"
     CDS             complement(<1..980)
                     /locus_tag="VNZ06_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HWW19571.1"
                     /translation="MRLASTLFVLCTAPSCAVLTACGHGTAQAVPHPAMSQVGTDLAS
                     EQTLTLSIDSAPTTLDPLLTTETQTQHILDDLFEGLVAIGIDGKPVPGVASDWAVSQD
                     GMTWTFHLRADARWSNGAPVTAQDFIYAWRRELDPKTGSAYGQSLAPLVNAEQISAGK
                     MPASALGVDSPDPRTLVIHLSAPVPYFLSILDQQYFYPLYEPAISQWGDQWTRPEHMV
                     SNGAFRLQEDAGNSRITLAKNSSYWDARNVRLEQVTYIVLTDQNIHSLRFQSGEVQFA
                     YFFPSSQYQWLKSHFGDQAVVAPFLGTLFIGFNLNLPPFRNNPALRRALS"
     gene            complement(991..3009)
                     /locus_tag="VNZ06_02095"
     CDS             complement(991..3009)
                     /locus_tag="VNZ06_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWW19572.1"
                     /translation="MEFIRGGPWMVDGILGKEADRLLAHRVIGSVAVSYVTSGVLLLG
                     FALAGTIGFVLPFAYAGCGLIECAVFYYLTDQASKIVEGYDYLIPQRLILSTAMQLVF
                     MALAPQIAFYFLTMLFVVYALGSIGITARQAAFTWLCVAVCTLVLFLRNKTGGVPHAT
                     LAERELVWLSFVVTLGRCVMLGVFGRMLRLRLQRRTLQLGETVAALKQRDQSLERVNA
                     ELLHQASHDSLTGLLNRVLFAERLKEAVMQSRPFAVCVLDLDRFKLINDSLGHGAGDA
                     LLKLIAQRLLSVMRSDDSVARAGGDEFLLLLRDVSSLEEIERLMIRSNEVLAETCKLH
                     GSDLHISSSIGVARFPADGTSGEELLARADEAMYHAKQNGRNMYRFFDAGIMGFSRER
                     LAIEAELRHAVSHGEFRLHYQPKIDIDSGEIRSVEALLRWQHAVRGRMMPGEFIAIAE
                     DTGLILSIGEWVIREACRQTRQWQLQGLPFLRVAVNVSPMQFRQANFLHVVSEALSSN
                     SLDPSSLEIELTEATLMSNAEKSVTMLERLSQLGVVVSIDDFGTGYSSMSYLQRFPID
                     KLKIDRSFIRDLNSNPDDASIVRAIISLAHGLRLKVVAEGVESAAQLELLKRMGCDQY
                     QGFLRSPAVPAAEVEAILADNQAQIRAMSLPGAERTYGKLARMLRGNA"
     gene            3241..4332
                     /locus_tag="VNZ06_02100"
     CDS             3241..4332
                     /locus_tag="VNZ06_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWW19573.1"
                     /translation="MRTDSAAERVTGPDRAAHAGSTTLVTWAKAICRALDAAGCDVPE
                     ILCRAGLDPTILNEPTARCPFEQGVELWRCAIEATGDDAFGLKVASQVRHTSFHALSY
                     GIIASSTLKEAFERICRYCHVVSDAVEYQLLRCGPEYHFVIHPMQNVPFASVDAAVAL
                     QLRMCRSFVGHDYSPLRIELRRPRPARVEEFQTLLRAPLQFGCEQNRLVFDCESMERL
                     LDSANPELARHNDAIALQYLARIERGNIQARVREVLMRRLECGEPSQEEIAALLSVSA
                     RTLQRRLGESGTTYKEILDETRHALALTYLSAPENSLSDVTYLLGFSAGSSFTRAFRR
                     WTGLSPSAWRARAARTPPPNSPSPGYLAA"
     gene            complement(4338..>4733)
                     /locus_tag="VNZ06_02105"
     CDS             complement(4338..>4733)
                     /locus_tag="VNZ06_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWW19574.1"
                     /translation="LKDGLRRLTGGKGADIIFDPVGGSYAEAALRSIAWNGRFLVVGF
                     PAGIPKIPLNLPLLKNCQIVGVFWGDSVARRPQQHAQNMAELLALYSEGRIRPMVTER
                     FPLARGGEAIARLGARATRGKLAVVMGES"
BASE COUNT          899 a         1499 c         1502 g          833 t
ORIGIN      
        1 atgctcaatg cgcgtcgcag cgcagggttg tttcgaaatg gcggcaaatt gaggttgaag
       61 ccgatgaaca gcgtgccgag gaagggcgca acaacggcct gatcaccgaa gtgtgacttc
      121 agccactgat actgtgacga aggaaagaag taggcgaact gcacttcgcc ggactgaaat
      181 ctcaacgagt gtatgttctg atcagtcagc acgatgtacg tgacctgctc gagccggaca
      241 ttgcgcgcat cccagtagct cgaattcttt gccagcgtga ttcggctgtt tccggcgtcc
      301 tcctgcagac gaaaggcgcc attgctgacc atgtgttccg gccgagtcca ctgatcaccc
      361 cactgggaaa tggcgggttc gtacagcgga tagaaatact gctggtcgag gatgctcagg
      421 aagtacggca cgggcgccga gagatggatc accagagtcc gcgggtcagg actgtcaacg
      481 ccgagagccg aagccggcat tttcccggcg ctgatctgct ccgcattcac cagcggtgcc
      541 agcgattgcc cgtaggcgga gcccgtcttc ggatccagtt cacgccgcca ggcatagatg
      601 aagtcctgtg ccgtgaccgg cgcaccattg gaccagcgcg cgtcggcgcg cagatggaac
      661 gtccaggtca tgccgtcctg gctgacggcc caatcgctgg caacgccggg taccggtttg
      721 ccatcgatgc ctatcgcgac cagaccttcg aacagatcat ccagaatgtg ctgggtctgc
      781 gtttcggtcg tcagaagcgg atcgagcgtg gtcggcgcac tgtcgatgct cagcgtgagg
      841 gtctgctcgc tcgccagatc agtcccgacc tgggacatag cgggatgggg aaccgcttgc
      901 gcagtgccgt ggccacaggc ggtcaggacg gcgcaggacg gcgccgtgca aagtacgaac
      961 agggtcgatg ccaggcgcat cggaatgctc ttacgcgttg cccctcaaca tacgcgccag
     1021 cttaccgtag gtgcgctcgg cgccaggcag agacatcgca cgaatctgag cctgattgtc
     1081 cgccagaatc gcttccacct cggctgccgg cactgccgga ctgcgcaaga agccctggta
     1141 ctgatcgcag cccatgcgct tgagcaactc gagctgagcg gccgattcca cgccttcggc
     1201 gacgactttc agccgcaggc cgtgcgcgag ggaaatgatt gcccgcacga tcgaggcgtc
     1261 gtccgggttg gaattcaggt cgcgaatgaa gctgcgatcg atcttcagct tgtcgatcgg
     1321 gaagcgctgc aggtagctca tgctcgagta gccggtgccg aaatcgtcga tcgacaccac
     1381 cacgccgagc tggctcaggc gctcgagcat ggtgacggac ttctcggcgt tgctcatcag
     1441 cgtcgcctcg gtcagctcaa tttcaaggct ggagggatcg agcgagttgc tgctgagcgc
     1501 ctcgctgacc acgtgcagga agttcgcctg acgaaactgc atcggcgaga cattcactgc
     1561 cacgcgcagg aagggcaggc cctgcagctg ccactggcgg gtctggcggc aggcttcgcg
     1621 gatcacccac tcgccgatcg acagaatcag tccggtatcc tccgcgatgg caatgaactc
     1681 gcccggcatc atgcggccgc gcaccgcgtg ctgccagcgc agcagcgcct cgacgctgcg
     1741 aatctcaccg ctgtcgatgt cgattttcgg ctgatagtgc agccggaatt caccgtggct
     1801 gacggcatgc cgcagttcgg cctcgatcgc cagccgctcg cgcgagaatc ccatgatgcc
     1861 ggcatcgaag aagcggtaca tgttccggcc gttctgcttg gcgtgataca tcgcctcgtc
     1921 ggcgcgggcc agcagttcct caccgctcgt gccgtccgcc gggaagcgcg cgacgccgat
     1981 gctggatgag atatgcaggt cgctgccatg cagcttgcat gtctcagcca gcacttcgtt
     2041 actgcggatc atcagacgct cgatttcttc cagggaactg acatcgcgca acagcagcag
     2101 gaattcgtca ccgccggcgc gcgcgaccga atcgtccgag cgcatgaccg acagcagtcg
     2161 ctgcgcgatc agtttcagca acgcgtcgcc ggcgccatgt ccgagtgaat cgttgatgag
     2221 cttgaagcga tccagatcca gcacgcacac cgcgaacgga cgcgactgca tcaccgcttc
     2281 cttcagccgc tcggcgaaca gtacgcgatt gagcagaccg gtcagtgaat cgtggctggc
     2341 ctgatgcagc agttcggcgt tgacgcgttc gagcgactgg tcgcgctgtt tcagggctgc
     2401 gacggtttca ccgagctgga gcgtgcggcg ctgcagccgc aggcgcaaca tccggccgaa
     2461 gaccccgagc atcacgcagc gtccgagcgt gacgacgaaa ctcagccaca ccagttctcg
     2521 ttcggccagg gtcgcgtgcg gcacgccgcc ggtcttgttt cgcaggaaca gcaccagggt
     2581 gcagaccgcg acgcacagcc aggtgaacgc cgcctggcgc gcagtgatgc cgatcgagcc
     2641 gagtgcatac acgacgaaca gcatcgtcag gaagtagaag gcgatctgcg gggccagtgc
     2701 catgaacacc agctgcatcg ccgtggacag gatcagtcgc tgcgggatca gatagtcgta
     2761 accctcgacg atcttcgagg cctgatcggt gaggtagtag aagaccgcgc attcgatcag
     2821 accgcaaccg gcataggcga acggtaatac gaatccgatc gtgccggcga gcgcgaaccc
     2881 taaaagcaac accccgctgg taacataact gacggcaacc gagccgataa cccggtgcgc
     2941 gagcaagcgg tcggcttctt ttcctagaat tccgtctacc atccaaggtc cgccgcggat
     3001 gaattccatg caccttacgc ggccggccca gcccgaaacg tgacgcattc gacaccgcgt
     3061 cgggagcctc gaatagtagt gacttcagtc atgccgacgc gtgacttaac gcggtcgtcg
     3121 caggcgcgca acgcgccgcg gcacacgatt cgacgatgcg cttctttcca gctaagctgc
     3181 ggcagtaggc ggggctggac cgcggtgggt gccagtctcg aatggcggtt tggggggatt
     3241 atgcggaccg acagtgccgc cgagcgcgtg accggacccg atcgggccgc gcacgccggc
     3301 agtacgacgc tcgtgacctg ggccaaagca atctgccgtg cactggatgc ggctggctgc
     3361 gacgttccgg aaatactctg ccgggcgggc ctcgacccga cgatcctgaa tgaaccgacc
     3421 gcccggtgtc cgttcgagca gggcgtggag ctgtggcgtt gcgccatcga agcgactggc
     3481 gatgatgcct tcggcctgaa agtggccagc caagtccggc acacctcttt ccacgcgctc
     3541 agttacggca tcattgccag ctccaccctg aaggaggcgt tcgagcgcat ctgccgctac
     3601 tgccatgtgg tcagcgatgc ggtcgagtac cagctgctgc gctgcgggcc cgaataccac
     3661 ttcgtgattc acccgatgca gaacgtaccg ttcgcgtcgg tcgacgcggc cgtggccctg
     3721 cagctgcgca tgtgccgctc gtttgtcggc catgactatt cgccgctgcg catcgaattg
     3781 cgccggccgc gaccggcacg ggtcgaggag ttccagacgt tgctgcgcgc gccgctgcag
     3841 tttggttgtg agcaaaatcg cctggtgttc gattgcgaga gcatggagcg tctgctggat
     3901 agcgcgaacc cggagctcgc gcggcacaac gatgcgatcg ctctgcagta cctggcccgc
     3961 atcgagcgcg gcaacatcca ggctcgggtg cgcgaagtgc tgatgcgccg actggagtgc
     4021 ggggagccct cgcaggaaga gatcgctgcc ctgctgagcg tgagcgcgcg cacgctacag
     4081 cgccggttgg gcgagagtgg caccacgtac aaggaaattc tggatgagac ccgccacgcg
     4141 ctggcgctga cttatctgag tgccccggaa aacagcctga gcgacgtcac ctatctgttg
     4201 ggcttctccg ccggcagcag cttcacccgc gcgttccgac gctggaccgg tctttcgccc
     4261 tcggcatggc gggcgcgcgc agcgcgcacg ccaccaccaa attccccctc accgggatat
     4321 ctggcggcgt agagctttca ggactcgccc atcacgaccg cgagcttgcc tcgggtagcg
     4381 cgcgccccca gccgcgcgat cgcctcaccg ccccgcgcca gcggaaagcg ttcggtcacc
     4441 atcggacgga tgcgtccctc gctatagagg gcgagcagtt ctgccatgtt ttgagcgtgc
     4501 tgctgggggc gacgggccac cgagtccccc cagaacacac cgacgatctg gcagttcttg
     4561 agcaatggca gattgagcgg gatcttcggg atgccggccg gaaatccgac caccaggaag
     4621 cggccgttcc atgcgatcga tcgcaacgcc gcctcggcgt agctgccgcc gacggggtcg
     4681 aaaataatgt cggcgccctt gccgccggtc agccgccgca agccgtcctt gag
//
LOCUS       DATMRM010000070         4675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3751873, whole genome shotgun sequence.
ACCESSION   DATMRM010000070 DATMRM010000000
VERSION     DATMRM010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3751873"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VNZ06_02110"
     CDS             <1..422
                     /locus_tag="VNZ06_02110"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HWW19575.1"
                     /translation="LDKQIEEINDLEAQKQKFISRMQIIEKLQRSRPEVVHLFDEMVK
                     IMPDGTYLTSVKESGNKLKFEGVAQSSTRVSSLMRNIADSQFLRNPDLEVVQTKNEAV
                     GNSFVLDAEQVSVINSDDTSQPGPRRPAHGAPAGAAK"
     gene            428..1075
                     /locus_tag="VNZ06_02115"
     CDS             428..1075
                     /locus_tag="VNZ06_02115"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HWW19576.1"
                     /translation="MDELRTLNVREPGRWPFVIHAISVLLCFLVVSGILVYVFVWEDR
                     MPALQQSQDQEMKLRAEFKDKHSKAANLSLYKQQLADIEKSFGALLRQLPSKTEVPNL
                     LVDISQTGLAAALEQKLFQPGQEVKKDFYAELPIHIQLTGSYHQFGSFVSGIAALPRI
                     VTLHDISITPLKQNGSFDQLQLDLTAKTYRYLDDDEVAAAEAEKRKSDAAKRHGA"
     gene            1078..1617
                     /locus_tag="VNZ06_02120"
     CDS             1078..1617
                     /locus_tag="VNZ06_02120"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HWW19577.1"
                     /translation="MSARYNRSHSRPVRGLGTLLGLALVVGALSGCSARDSDLQRFID
                     TTKHEPGGRVEPLPEVKPYEAYGYADQELRSPFVPGGSGGANANLRPDSHRNREYLEQ
                     FSLDTLKMVGTLNLGGGHYGLVQTKDGRVHRVVIGEHMGTNDGKITDISASKIALVEI
                     IPDGLGGYIERPAALALNE"
     gene            1638..3836
                     /locus_tag="VNZ06_02125"
     CDS             1638..3836
                     /locus_tag="VNZ06_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HWW19578.1"
                     /translation="MKRSILHTLRGRIALLALACAAGTLAAPGARAADAPAGANQLES
                     ISVQSLNGTQTQLTLHLSAAAPEPLVFTIDKPARLSLDLPNTSLALPSRRIDVGTGGI
                     DTVLAAEANGRSRVVLNLDQQLPYTTSVNGNDIILVVGTGAKAAKSGAAAAASSAPMA
                     ASSAARAIRNVDFRRSETGAGRLIVRLSDPHTPIDLKQQGNQLLLTFAGTDLPKKYSR
                     RFDTLDFATPVTGFDVGRDSSGDTRIVINATGDFEHLAYQSDDQYVVDISPVRKAPGE
                     VANEKKEYKGERLTLNFQDIEVRAVLQLLADSSGQNIVVSDSVGGNLTLRLQNVPWDQ
                     ALDIVLRTKGLDKRQEGNVIIVAPAEELATREKADLASKKDIQELEPLRSEYLQVNYA
                     KASDISNLLKGVTPGSTGGGQASGSVLSQRGKVSVDDRTNTLLLQDTAEKLTEIRRLV
                     ATLDIPVKQVLIETRIVVVTNDFERDLGARFGVTAARANGTNGLFETTGTAAGVDTGV
                     SSALNNLASSGSVFPINIPVGTAATNRYNVNLPVASPAGSIAFGILGSDFLVDLELSA
                     AETENRGTVVASPRVVTANQKEATIQQGVEIPYQQSASSGATTIAFKDAVLLLKVKPL
                     ITPDNRIILDLTVSDDTVGQVVVASGGVNVPSINTRKIETQVVLSDGQTVVLGGILTT
                     NQTLQATKVPWLGDIPVLGNLFKSTTNTNDKDELLIFVTPKIIREGVTVN"
     gene            3913..>4675
                     /locus_tag="VNZ06_02130"
     CDS             3913..>4675
                     /locus_tag="VNZ06_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWW19579.1"
                     /translation="MRKLGCILAALSVLALSACGGGSLSTGTNTGTHTGSSSGSSSSS
                     GGINATVATVKVSSSAASVPADGSAGATITAQTLDANNNAVSGATVTFSTSTGSVLTN
                     ETTTTDVAGHATATVTDSGGAAGSTITVSATAGTAKGSANVMVVSNQQSVTLLVSSPS
                     LPSNGSKPVTIQAIVRNASNQLVTGAVVSFSATSGGITPVTTTAGAAATPAVPAGTTD
                     ANGAAAAQLTPAGDPTNRTITVTATVGSTQATAAVA"
BASE COUNT          966 a         1430 c         1458 g          821 t
ORIGIN      
        1 tgctggacaa gcagatcgag gagatcaacg atctcgaggc gcagaagcag aagttcattt
       61 ctcgcatgca gatcatcgag aagttgcagc gttcgcgacc tgaagtcgtg cacctgttcg
      121 atgaaatggt caagatcatg ccggacggga cctatctgac ctcggtcaaa gagagcggca
      181 acaagctcaa attcgagggc gtcgcgcagt cctcgactcg cgtctcctca ctgatgcgca
      241 acatcgccga ctcgcagttt ctgcgtaatc ccgatctcga ggtcgtgcag accaagaacg
      301 aagccgtcgg caacagcttc gtgctcgacg cggagcaggt cagcgtgatc aatagcgacg
      361 acacatcgca gccgggaccc agacgtccgg cacatggtgc cccggcggga gcagctaaat
      421 gaacctgatg gatgaactgc gcacgctgaa cgtgcgcgaa ccgggtcgtt ggccgttcgt
      481 gattcatgcg atttcggtgc tgctgtgttt cctggtggtg agcggcattc tggtttatgt
      541 gttcgtgtgg gaagaccgga tgccggccct gcagcagtcg caggatcagg aaatgaagct
      601 gcgcgctgag ttcaaggaca agcactccaa ggccgccaac ctcagtctgt acaagcaaca
      661 gctggcggat atcgagaagt cattcggtgc gctgctgcgc caattgccca gcaagaccga
      721 ggtccccaat ctgctggtgg atatctcaca gacgggtctg gcggcggcac tggagcagaa
      781 attgttccag cctggtcagg aagtgaagaa ggacttctat gccgagctgc cgatccacat
      841 ccagctcacc ggcagctacc accagttcgg ctcgtttgtc agcggcattg ccgcgctgcc
      901 acgcatcgtg acgctgcacg acatttcgat cacgccgctc aagcagaatg gcagtttcga
      961 tcagctgcag ctcgatctga ctgccaagac ctatcgctat ctggatgatg acgaagtcgc
     1021 cgccgcggaa gcagaaaagc gtaagtccga tgcggccaaa cggcatggag cctgatgatg
     1081 tcggcacgat ataacaggtc gcattcgagg ccggtgcgtg gactgggaac gctgctcgga
     1141 ctggcgctag tggtcggtgc gctgagcggc tgcagtgccc gcgactccga tctgcagagg
     1201 ttcatcgata ccaccaagca tgagccgggt ggtcgcgtcg aaccgctgcc tgaggtgaaa
     1261 ccctatgagg cgtacggata cgctgatcag gagctgcgtt cgccgttcgt ccccggcggt
     1321 tcgggcggcg cgaacgccaa tctgcgcccc gactcgcacc gcaatcgtga gtatctggaa
     1381 cagttttcgc tcgatacgct gaagatggtg ggcacattga atctgggcgg cggccactac
     1441 ggcctcgtcc agaccaagga cggccgcgtg caccgtgtgg tgatcggtga gcacatggga
     1501 accaacgacg ggaagatcac cgacatttca gcctcgaaga ttgctttggt tgaaatcatt
     1561 cccgacggac tgggcgggta cattgaacgt cctgcggccc tggcgttgaa cgaatgattg
     1621 gcatggagac acatcgaatg aaacggtcca tcctccacac gctgcgtgga cgcattgctc
     1681 tgctagccct ggcttgcgcg gcaggaacgc tcgccgcacc gggcgcacgc gcggcggatg
     1741 ctccagccgg cgccaatcag ctggagtcca tcagtgtgca gtcgctcaat ggcactcaga
     1801 cacagctgac gctacacctg agtgcggcgg cgcccgagcc gctggtattt accatcgaca
     1861 agccggcccg gctgtcgctt gacctgccga acacttcgct ggcattgccg tcgcgtcgca
     1921 tcgacgtcgg caccggcggc atcgacaccg tactggcggc cgaagcgaac ggccgctcgc
     1981 gtgtggtgct gaatctcgat cagcagttgc cctacacgac ctcggtcaat ggcaatgaca
     2041 tcattctggt ggtcggcacg ggagccaagg ccgcgaagag cggcgctgcg gccgccgcta
     2101 gcagcgcgcc gatggctgcc agcagtgctg ctcgcgcgat caggaacgtc gactttcgtc
     2161 gcagtgagac cggtgccgga cgattgatcg tgcgcctgtc cgatccacac acgccgatcg
     2221 atctgaagca gcagggcaac cagctgctgc tcacctttgc gggcaccgat ctgcccaaga
     2281 agtacagccg gcgctttgac accctggact tcgccacgcc ggtgacaggc ttcgatgtcg
     2341 gccgcgactc cagcggcgat acgcgcatcg tgatcaacgc caccggtgac tttgagcatc
     2401 tggcctatca gtccgacgat caatacgtgg tggacatctc gccggttcgc aaagcaccgg
     2461 gcgaggtcgc gaacgaaaag aaggaataca agggcgagcg tctgacgctg aacttccagg
     2521 acatcgaagt tcgcgcagtg ttgcagctgc tggccgattc gagtggccag aacatcgtgg
     2581 tcagcgacag tgtcggtggc aacctgacgt tgcgacttca gaatgtgccc tgggatcagg
     2641 cgctggacat cgtgctgcgt accaagggcc tggataagcg ccaggaaggc aacgtcatca
     2701 tcgtcgcgcc ggccgaggag ctggccacgc gtgaaaaagc ggatctggcc agcaagaagg
     2761 acatccagga actcgagccg ctgcgctccg agtacttgca ggtcaactat gccaaggctt
     2821 cggacatctc gaacctgctg aagggcgtga cgcccggcag taccggcggc gggcaggcat
     2881 caggctcggt gctttcgcaa cgcggtaagg tgtcggtgga tgaccgcacc aacacgctgc
     2941 tgctgcagga cacggcagag aaattgacag agattcgccg cctggtggcc acgctcgaca
     3001 ttcccgtcaa gcaggtgctg atcgagacgc gcatcgtggt cgtgaccaac gacttcgagc
     3061 gtgacctcgg tgctcgcttc ggcgtgaccg cggcgcgcgc caacggtacc aacggtctgt
     3121 tcgagaccac cggcacggcc gcgggcgtgg ataccggcgt gagttccgcg ctcaacaatc
     3181 tggcgagcag cggctcggtg ttcccgatca acattccggt cggcaccgcc gcgaccaacc
     3241 gatacaacgt caaccttccg gtggcaagtc cggcgggcag cattgcgttc ggcatcctcg
     3301 gctccgattt cctggtggac ctcgaactgt ccgccgctga gaccgaaaac cgcggaacgg
     3361 tcgtcgcctc gccgcgtgtg gtcacggcga accagaagga agccaccatc cagcagggtg
     3421 tggaaattcc ctaccagcag tcggcatcca gtggcgcgac caccatcgca ttcaaagatg
     3481 cggtgctgtt gttgaaggtg aagccgctga tcactcccga caatcgcatc attctggatc
     3541 tgaccgtctc ggatgacacg gtcggacaag tcgtcgtggc ttccggcggc gtgaatgtgc
     3601 cgtcgatcaa cacacgcaag attgaaacac aggtcgtcct gagcgacggc cagaccgttg
     3661 tgctgggcgg catcctgacg accaatcaga cgctgcaggc gaccaaagtg ccgtggctgg
     3721 gcgacatccc ggtgctcggc aatttgttca agagcacgac caataccaat gacaaggatg
     3781 agctgttgat cttcgtgacg ccgaagatca ttcgagaagg cgttactgtg aactgattat
     3841 ctgccgggaa aatgggctga cactagtcta cgtccttatg tcttgaggct cagacgattt
     3901 atggtgacta cgatgcgcaa attgggatgc atattggcgg cattgagcgt gctggcgttg
     3961 agcgcctgcg gcggtggcag tctcagcact gggaccaaca ccggaacgca cacgggcagc
     4021 tcgtccggga gcagcagcag cagcggcggc atcaacgcga ccgtcgccac tgtcaaagtg
     4081 agctccagcg ccgccagcgt gccggccgac ggctctgccg gggccaccat taccgcgcag
     4141 accctggacg ccaacaacaa cgcggtctcg ggcgcgacgg tgaccttctc gaccagcacc
     4201 ggctcggtac tcaccaacga gaccacgacc accgatgtcg ccggtcacgc gactgcgacg
     4261 gtcacggaca gcggcggtgc cgcgggcagc accatcaccg tctcggcgac cgcaggcacc
     4321 gccaagggct ccgccaacgt catggtggtc agcaaccagc agtcagttac tctgttggtt
     4381 agctcgccga gcctaccgtc gaacggatcc aagccggtca cgattcaggc catcgtgcgc
     4441 aacgcgagca accagctcgt gacgggcgca gttgtgagct tctccgcgac ctcgggtggc
     4501 atcacaccag tgacgacgac tgctggcgcg gcggcaactc ccgcggtacc ggctggaacc
     4561 accgatgcga acggcgccgc cgccgcacag ctcaccccgg cgggtgaccc caccaatcgc
     4621 acgatcacgg tgacggcgac ggtgggaagc acgcaggcga ccgccgctgt ggccg
//
LOCUS       DATMRM010000071         2496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3442883, whole genome shotgun sequence.
ACCESSION   DATMRM010000071 DATMRM010000000
VERSION     DATMRM010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3442883"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VNZ06_02135"
     CDS             <1..241
                     /locus_tag="VNZ06_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19580.1"
                     /translation="YWYGGNYHAPWFAAFDGLVTLAIIIGLGWWSLHHMNDIREFFQN
                     LPHLWNKDTWSNTAARIVVKLCTTMKDWLLISPQA"
     gene            complement(393..1754)
                     /locus_tag="VNZ06_02140"
     CDS             complement(393..1754)
                     /locus_tag="VNZ06_02140"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWW19581.1"
                     /translation="MNANRILVVDDESDIRRLLQEILTEEGYDVEVAADAGQARAARA
                     RQIPDLVLLDIWMPDTDGITLLREWSNEASDSCPVVMMSGHGTVETAVEATRLGAYDF
                     VEKPLSLAKLLRTVERALDAKARRPTGKFASASTNGIVAPLGRSRTMTNLRSELTRMA
                     AFNTPLLLLGESGTDREMLARFVHQSGPTASGPFVVLDSRTLRDDNAAVTLLGMSASQ
                     PGLFDQARKGTLYLGDIEDLPDETQRLLSGVLEAGKYVRTGGGDPVTQETRIISSARP
                     GIVNHVLADDFRRDLYAQLSVLVVRVPALRDYAEDVPELLRQYVDELTETEGLRFRRF
                     SVAAQNRLRNYPWPDNVRELRNLTRRFLHNSGPEEIGLEEVERELSSQTPADEPLVKH
                     DLLALPLREAREQFERAYLQQQLMLCNGKVGLLAKRVDMERTHLYRKLRSLGIEIGHG
                     AED"
     gene            complement(1751..>2496)
                     /locus_tag="VNZ06_02145"
     CDS             complement(1751..>2496)
                     /locus_tag="VNZ06_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW19582.1"
                     /translation="DAAWGEVARRLAHEIKNPLTPIQLSADRLRRKLLGSMNAEDATL
                     LERATHTIVQQVDAMKQMVNAFSEYARSPDMKLQHFNLNQLVNEVAELYRLQDPSIEI
                     RLDLDPGLPELEADRGRVRQILANLITNGLEALTGLQAGCLEISTRQQRAEGAASAVI
                     CVRDNGPGFRKEILGRLFDPYVTGKPRGTGLGLAIVKRIVEEHGGRIEAENRPEGGAK
                     ILVFLPVSDQDRSLLAGGERRMELRRERA"
BASE COUNT          436 a          836 c          739 g          485 t
ORIGIN      
        1 ttactggtat ggtggcaact atcacgcccc gtggttcgcg gcattcgatg gcctcgtcac
       61 gctcgcgatc atcattggac tgggctggtg gagcctgcac cacatgaacg acatccgaga
      121 gtttttccag aacctgccgc acctgtggaa caaagacaca tggtcgaaca cggcggctag
      181 gatcgtcgtg aagctctgca cgacgatgaa ggactggctg ctgatcagcc cgcaggcctg
      241 aagtagggcc ggtgcgcccc gacgctgcag caaggcccgc tccggtccgg cgctctgttc
      301 ccatgctcgt aactcatccc gagcagctgc atgctcacgg ccacccgccg caggagtggc
      361 gagtccagca ttcctatcaa caacgctggg cgctagtcct ccgcaccatg gccgatttca
      421 atgccgagcg aacgcagctt acggtacagg tgggtgcgct ccatatcgac ccgctttgca
      481 agcagtccga ccttgccatt gcagagcatg agttgctgtt gaaggtaggc gcgctcgaac
      541 tgctcacgcg cctcacgcaa cggcagcgcc agcagatcgt gcttcaccag cggctcatcg
      601 gccggcgtct gcgatgacag ctcgcgctcg acttcctcca gtccgatctc ctcaggaccc
      661 gagttgtgca gaaagcgccg cgtgagattg cgcagctcgc gcacgttgtc gggccacgga
      721 taatttcgta gccggttctg cgccgccacg ctgaagcgcc ggaaacgcag tccctcggtc
      781 tcggtcagtt catcgacata ctgacgcagc agttccggca catcctcggc gtagtcgcgg
      841 agcgccggca cacgcaccac cagcacggac agttgcgcgt acaggtcgcg gcggaaatca
      901 tcggccagca catggttgac gatgcctgga cgcgcggagg aaatgatgcg cgtctcctgc
      961 gtcaccggat cgccaccacc cgtgcgcaca tacttgcccg cctccagtac cccgctcagc
     1021 aggcgctggg tttcgtcggg cagatcctcg atgtctccca ggtacagcgt gcccttgcgg
     1081 gcctgatcga acaggccggg ctgtgaggcg ctcataccca acagtgtcac cgcggcgttg
     1141 tcatcacgca gggtgcggct gtcgagcacc acgaacggcc cggatgccgt gggtccggac
     1201 tgatgaacga agcgcgcgag catttcgcga tcggttcctg attccccaag cagcagcagc
     1261 ggggtattga acgccgccat acgagtcagc tcgctgcgca gattggtcat cgtgcggctg
     1321 cgacccagcg gcgcgacgat gccattggtg ctcgcgctgg caaatttgcc ggtgggacgc
     1381 cgcgccttcg cgtccagggc gcgctccacg gttcgcagca gcttcgcgag cgacagtggc
     1441 ttctcgacga aatcgtaggc gcccagacgg gtcgcctcga ccgccgtctc gaccgtgcca
     1501 tgacccgaca tcatcacgac cggacaggaa tcgctcgcct cgttggacca ttcacgcagc
     1561 agcgtgatgc cgtcggtatc cggcatccag atgtcgagca gcaccagatc gggaatctgc
     1621 cgcgcacgcg cggcacgcgc ctgacccgca tccgcggcca cttcgacgtc gtagccttcc
     1681 tctgtgagta tttcctgcaa caggcgacga atgtcggact cgtcgtccac aaccaggatt
     1741 ctgttggcgt tcatgcacgc tccctacgca attccatgcg tctttctccc cccgctagca
     1801 gcgaccgatc ctgatcgctg accggtagaa ataccagtat tttcgccccg ccttccggac
     1861 ggttttctgc ttcgattcgc ccgccgtgct cttcgacgat tcgcttgacg atcgccagtc
     1921 ccaggccggt gcccctgggt ttgccggtca cgtaggggtc gaagagcctg cccagtattt
     1981 ccttcctgaa cccggggcca ttgtcgcgca cacagattac agcgctggcg gccccttctg
     2041 cacgctgttg tcgcgtactg atctcgaggc accctgcctg gagcccggtc aaggcttcca
     2101 ggccattggt aatcagattt gccaatatct gccgcacccg gccacggtcg gcttccagtt
     2161 cgggcagccc cgggtccaga tccagccgaa tctcgatcga tgggtcctga agccggtaca
     2221 gctcagcgac ctcgttcacc agctggttca gattgaagtg ctgcagcttc atgtccggcg
     2281 aacgcgcgta ctcggagaac gcgttcacca tctgcttcat cgcatcgacc tgttgcacga
     2341 tggtgtgggt cgcgcgctcg agcagcgtgg catcctcagc gttcatgctg cccagcagtt
     2401 tgcggcgcag ccggtcggcg gatagctgaa tcggtgtcag cgggtttttg atctcgtgcg
     2461 ccagccgccg ggcgacctcg ccccaggccg catcgc
//
LOCUS       DATMRM010000072         5787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3129507, whole genome shotgun sequence.
ACCESSION   DATMRM010000072 DATMRM010000000
VERSION     DATMRM010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3129507"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            413..706
                     /locus_tag="VNZ06_02150"
     CDS             413..706
                     /locus_tag="VNZ06_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19583.1"
                     /translation="MQKKPYRIRVTPWSAVRLVTHGDACARAAALHDKRFLAREAPAL
                     PLKGCTLASACNCMYKHYVDRRTGPRRDEETTGRRIVKAPVERRSARGRRGSD"
     gene            718..2094
                     /locus_tag="VNZ06_02155"
     CDS             718..2094
                     /locus_tag="VNZ06_02155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW19584.1"
                     /translation="MLDGLPTPRRYLAILAVSFGTGLAVLDGSIVTVALPTMARDLHV
                     ESSTSVLVVTIYQLTLVMTLLPLSALGSHLGLRRVYQCGQLIFLLSTALCFFARSMPF
                     LLVVRTIQALGAGAALSVSSALIRSIYPSRQLGLGLGISGVVVSVAGVIAPTLGGLVL
                     SVARWPWIFVAACPFALVSLLVGRHSLPDALPSDEPFDVLGAVLCAATFGLTISGLES
                     LVHGDSPVIAGFICVLGLGLAVVFVRRELHSKRPILPVDLLTRPIIGLSAIGGLVAFM
                     GSTTMIFSIPFRLQQQFGWLPLEVGAVIAPWPAAMAFAGFVAGILSDRFPAGILGAIG
                     MLIAVCGMLLLAFLPQHPTHFDMVWRMALCGCGYGLFLSPNSRLIIHAASRERAASAG
                     GLVGTTRLTGQTLGATLLAALLAAGVGRGPAPALAAASLALTAGVCSIARLRAAAYAS
                     GQASVQEI"
     gene            complement(2082..2849)
                     /locus_tag="VNZ06_02160"
     CDS             complement(2082..2849)
                     /locus_tag="VNZ06_02160"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HWW19585.1"
                     /translation="MCLILVAWRAHPDYPLVVAANRDEYHARAAAPASWWTHPPILAG
                     RDLTAGGTWLAVARDARFAALTNYRDPSRPPRDAPSRGALVPDTLQRMLPVAERLQRL
                     ERDASVYNGFNLIFSDGEQLAVFESVVGRGRVLTPGVYGLSNHLLDTPWPKVVAAKAA
                     LGAALTRLPEQQPLLALLRDEQQADDARLPRTGVSLEWERLLSSAFIRASGYGTRCST
                     LVLQDRRGSVQFAEWSWDPSGAPAGQVSFAFERAQIS"
     gene            complement(2862..3734)
                     /locus_tag="VNZ06_02165"
     CDS             complement(2862..3734)
                     /locus_tag="VNZ06_02165"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HWW19586.1"
                     /translation="MRWQGNRESDNVEDRRGESGGFGGIHLGIGGTVLVLLGAWLFGY
                     NPLTILSLLTGEGSSSPQVADVPPPGQPSPQGADPLRHFVGTILASTEDVWSVIFAQS
                     GRRYQPPHLVLFSNAIQTGCGGGQSAMGPFYCPQDQRVYIDLAFYRTLRTRLGSPGDS
                     AQAYVIAHEVGHHVQYLTGITNKVDALRARENRVQTNATSVRVELQADCYAGVWAFHS
                     QDQKHWFEDNEIGMVLNAASHIGDDALQKMEQGRVMPETFTHGTSQQRVNWFKRGYAS
                     GRVEDCDTFNAATL"
     gene            complement(3747..4382)
                     /locus_tag="VNZ06_02170"
     CDS             complement(3747..4382)
                     /locus_tag="VNZ06_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWW19587.1"
                     /translation="MADEIVFYTSPMSRGRMIQWMLEETGAPSRVELVDLQKNEQKNP
                     KFLAINPMGKVPAIVHRGVVVTECAAICTYLADAFPAAQLAPKSDDPARGTYLRWMFF
                     GAGCVDPGLMDRMLQRPTPERYQALGYGRFEDMIAVLEKAITPGPYILGERFSAADLY
                     ISAQIGFGLMTKTLEASPAFQAYAARCNDRPAYQRALAKTEELTVKMKAAS"
     gene            complement(4432..5277)
                     /locus_tag="VNZ06_02175"
     CDS             complement(4432..5277)
                     /locus_tag="VNZ06_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWW19588.1"
                     /translation="MDNRNTPKVSDATMAVFLDLENIALGARDANYPSFDIQKVLGRL
                     LLKGHIVVKKAYCDFERYKEFKRGLHEAAFELIEIPHVRQSGKNSADIRMVVDALDLC
                     YTKGHVDSFVIISGDSDFSPLVSKLRENDKTVIGVGVRNSTSDLFINNCDEFIYYDDL
                     VRTKEPARSRRRAPSATTTNAATSPANGEAKPTDPSEGLDLVIETLEAIADERGGDAI
                     WGSMIKQAIKRRNPGFNERAHGFKSFNDLLIEAQKRGFIDLQPDERSGGYTVTLADRG
                     AARPV"
     gene            complement(5321..>5787)
                     /locus_tag="VNZ06_02180"
     CDS             complement(5321..>5787)
                     /locus_tag="VNZ06_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HWW19589.1"
                     /translation="VNGDGYDDLIITDPGPYGGAAPFVAVLLQNPASPGTFLAPVNYP
                     TAANSLAQSIQVVDINGDGRPDIIIGGSNAVTVLLNSASSPGTFGAAANYAAVDANQI
                     AVADVNGDGLLDIVTATGATHPLVNNVYTNGPGVLLQSAAAPGTFQPLQDLN"
BASE COUNT          955 a         1815 c         1850 g         1167 t
ORIGIN      
        1 ctcacccgtg gaatacgacc agtgcagtgt caaccacgta aagccagcga ctccaaggcc
       61 agcgagcaac accagcccga ggaagatcct gatcataggt tcatggagtc cgtgtattgt
      121 ttgaccagac gctcttatga attatagcga ccgactgagc gatgcgagaa taatcgagtc
      181 cgagcacaag aatgccacgc cctggatcgg cgggagggcg gctggcaggg atttaacagc
      241 cgccttcagc cacgctccgc cctggtactt tgcgcacact gggcgggccg gataaggctg
      301 ttgcgtgagc cgttacagcc cgtcagtggt cgtccggcgt cggtgatatt tgttgccggc
      361 gtgatgcgct agtcatatcg tgcacgttcg gacgcttaga atgcctcggc ctatgcagaa
      421 gaagccctac cgcatacgag tcaccccctg gagcgccgtg cgtctggtga cccatggcga
      481 tgcctgtgcc cgggcggcgg cgttgcatga caagcgcttt ctcgcacgcg aggctcctgc
      541 actgccgttg aaggggtgca cgctcgccag cgcctgcaac tgcatgtaca agcactatgt
      601 ggaccgacga acaggtccgc gtcgcgatga ggaaaccact gggcgacgca tcgtgaaggc
      661 cccggtggag cgacgcagcg cgcgcggacg acgcggttca gattgacggg gtcgggcgtg
      721 cttgacggtc tgccgactcc ccgtcggtac ctcgcaattc tcgcggtgtc gtttggcacc
      781 ggactggcgg tactcgatgg atcgatcgtc accgtggcgc tgcccaccat ggcgcgcgat
      841 ctgcacgtcg aatcctcgac ctcggtgctg gtcgttacga tttatcagct gacgctggtg
      901 atgacgctgc tgccgctgtc ggcgctcggc agccacctgg gactgcggcg cgtctatcag
      961 tgcgggcaac tgatcttcct gctgtccact gcgctctgtt tcttcgcgcg cagcatgccg
     1021 ttcctgctgg tggtgcgcac cattcaggcg ctgggtgcgg gcgccgcgtt gagcgtctcg
     1081 agcgcattga tccgctcgat ctatccgtcg cgtcagctcg gcctgggtct gggcatcagc
     1141 ggtgtcgtgg tatcggtcgc gggagtgatc gcaccgacgc tgggtggcct ggtgttgtcg
     1201 gtcgcccgct ggccgtggat cttcgttgcc gcctgcccgt ttgcactggt gtcgctgctg
     1261 gtcggccgcc attcattgcc ggatgcgctg cccagcgacg aacccttcga tgtgctcgga
     1321 gcagtgctct gcgccgcgac attcgggttg acgatctcgg ggctcgaaag cctggtgcac
     1381 ggcgactcgc ccgtgatagc ggggttcatt tgtgtgctcg gtctgggcct ggccgttgtg
     1441 tttgttcgcc gtgaactgca ctcgaagcgg ccgattctgc cggtagatct gttgacgcgt
     1501 ccgatcatcg gcctgtcggc gatcggaggt ctggtcgcct tcatgggctc gaccacgatg
     1561 atcttttcca tcccattccg cttgcagcag cagttcggct ggctgccgct ggaggtcggc
     1621 gcggtgatcg cgccctggcc cgcagcgatg gccttcgcgg gtttcgtcgc cggaatcctg
     1681 tccgatcgtt ttccagcggg gatcctcggc gctatcggca tgctgatcgc tgtttgcggg
     1741 atgctgctgc tcgcctttct gccgcagcat ccgacgcatt tcgacatggt gtggcgaatg
     1801 gcactgtgcg gttgcggcta cgggttgttt ctgtcgccga acagccgtct gatcattcac
     1861 gcagcatcgc gcgaacgagc ggcctcagcg ggcggattgg tgggtacgac acgcctgacc
     1921 ggacagaccc tcggtgccac gctgctggcc gcgctgttgg ccgcgggtgt gggtcgtgga
     1981 ccggcgccgg cgctggctgc cgcctcactg gcgctgaccg caggggtttg cagcatcgcc
     2041 cgactgcgcg ctgccgcgta cgcgtccggt caggcgagcg ttcaggaaat ctgagctcgc
     2101 tcgaaggcga agctgacctg cccggcgggg gctccgctcg gatcccagct ccactcggca
     2161 aactgcacgc taccgcgccg gtcctgcaac accagagtcg agcagcgtgt accgtagccg
     2221 gacgcgcgaa tgaacgcact tgacagcagt cgctcccatt cgaggctcac gccagtgcgc
     2281 ggaagccgtg catcgtcggc ctgctgctca tcgcgcagca gcgcgagcag cggttgctgc
     2341 tcgggcaggc gcgtcagggc ggcgccgagg gcggctttcg cggccaccac ctttggccac
     2401 ggcgtgtcga gcagatgatt cgatagtccg tacacgcccg gcgtgagcac ccgcccgcgt
     2461 ccgaccacac tctcgaacac ggccagttgt tcgccgtcgc tgaagatcag attgaatccg
     2521 ttgtacacgc tggcgtcgcg ctccagccgt tgcagccgct cggcaaccgg aagcatccgc
     2581 tgcagcgtgt caggtaccag tgcgccgcga ctgggcgcat cacgcggcgg gcgcgaaggg
     2641 tcgcgatagt tggtgagtgc ggcgaaacgt gcatcgcgcg ctaccgcgag ccaggtgcca
     2701 ccggcagtca ggtcacgacc ggccagtatc ggcggatgcg tccaccagct cgccggcgct
     2761 gcagcacgcg catggtattc gtcgcgattc gccgcgacga ccagtggata gtccggatga
     2821 gcgcgccagg cgacgaggat cagacacatc gaggagcgat gtcaaagcgt ggcagcgttg
     2881 aaggtgtcgc agtcctcgac gcggccgctg gcgtaaccgc gtttgaacca gttcacgcgc
     2941 tgctggctgg ttccatgcgt gaaggtttcg ggcatgaccc ggccctgctc catcttctgc
     3001 agagcgtcgt cgccgatgtg cgaggccgcg ttgagaacca tgccgatctc gttgtcctcg
     3061 aaccaatgct tctgatcctg cgagtgaaag gcccatacgc ccgcatagca gtccgcctgc
     3121 agctctacgc gtaccgaggt agcgttggtc tgtacgcgat tttcacgcgc gcgcaacgca
     3181 tcgactttgt tggtgattcc ggtcagatac tggacgtggt gacccacctc gtgcgcgatc
     3241 acgtatgcct gcgccgagtc tcccggtgaa ccgagccgcg tgcgtagcgt ccggtagaac
     3301 gcgagatcga tgtagacgcg ttggtcctga gggcaataaa acggtcccat cgcgctctga
     3361 ccgcccccac atccggtttg gatggcgttg ctgaacagca ccagatgggg cggctgataa
     3421 cggcgtcccg actgcgcaaa gatcacgctc cagacatctt cggtgctggc gaggatggtc
     3481 ccgacgaaat gccgcagcgg atccgcgccc tgcggcgagg gctggcccgg tggcgggacg
     3541 tcggccacct gtgggctgct gctgccctcc ccggtcagca ggctcaagat cgtcagcggg
     3601 ttgtagccga acagccaggc tcccagcagc accagcacgg tgccgccaat ccccaggtga
     3661 atgccgccga aaccgccact ctcgccgcgt cgatcctcga cgttgtcgct ctcgcggttc
     3721 ccttgccagc gcatgatggt cggctgtcag ctggcggcct tcatcttgac ggtcaattct
     3781 tccgtctttg ccagcgcgcg ctggtacgcg ggccgatcgt tgcagcgagc ggcgtaggcc
     3841 tggaaggcag ggcttgcctc cagggtcttc gtcatcaggc cgaaaccgat ctgcgcgctg
     3901 atgtagaggt cagccgcgct gaatcgctcg ccgaggatgt acggtcctgg cgtgatcgcc
     3961 ttttcaagca ctgcaatcat gtcctcgaag cgaccatagc ccagcgcctg gtatcgttcc
     4021 ggcgtcggac gctgcagcat gcggtccatc agcccaggat cgacgcagcc cgcgccgaag
     4081 aacatccaac gtagataggt accgcgcgcc ggatcgtcgc tcttcggcgc cagttgtgcc
     4141 gccggaaagg cgtccgccag ataagtgcag attgcggcac actctgtgac gacgactccg
     4201 cggtgcacga tggcgggcac cttgcccatg ggattgattg ccaggaattt cggattcttc
     4261 tgttcattct tctgcagatc caccagttcg acgcgagaag gagcacccgt ttcctcgagc
     4321 atccactgaa tcatgcgtcc ccgcgacatc ggactggtat agaaaacgat ttcgtcggcc
     4381 atgatttatt tctccgcgcc acccggtgcc ggcgacctta gcacggaatc tctacaccgg
     4441 tcgcgctgcc ccccggtccg cgagcgtgac cgtataaccc cccgatcgct cgtccggctg
     4501 caggtcgatg aaaccccgtt tctgggcttc tatcagcaga tcattgaagg acttgaaccc
     4561 gtgtgcgcgc tcgttaaatc ccgggttccg gcgcttgatg gcttgcttga tcatcgatcc
     4621 ccagatggcg tcgccgccgc gttcgtcggc aatggcctcg agcgtctcga tgaccagatc
     4681 gagtccctca ctggggtccg tcggcttggc ctcaccgttg gcagggctgg tcgcggcgtt
     4741 ggtagtcgtg gcggacgggg cgcggcgacg tgacctcgca ggctccttgg tgcgtaccag
     4801 atcatcgtag tagatgaact cgtcgcaatt gttgatgaaa agatcggaag tggaattcct
     4861 gacgccgaca ccgatgaccg tcttgtcatt ctcgcgcagc ttgctgacca gcggagagaa
     4921 gtcggaatcg ccgctgatga tcacgaaact gtctacatgt cccttggtgt agcacaggtc
     4981 gagcgcatcc acgaccatgc ggatgtcggc cgagttcttg cccgactgac gcacatgcgg
     5041 aatctcgatc agctcgaagg ccgcctcatg cagcccacgt ttgaattcct tgtagcgttc
     5101 gaagtcgcag tacgccttct tgacgacgat gtggcctttg agcagtaagc gcccgagtac
     5161 cttctggatg tcgaaactcg ggtagttggc atcgcgcgcg ccgagagcga tgttctccaa
     5221 atcgagaaac accgccatcg tcgcgtcgct gaccttgggc gtattgcgat tgtccatccg
     5281 gtcatcatga ccgctcggca gggctatggc gagtgcgccg tcagttcagg tcctgcagcg
     5341 gctggaaggt gcctggcgct gccgcgcttt gcagcagcac gcccggtccg ttggtgtaca
     5401 cgttattgac caacggatgc gtcgcgccgg tcgcggtcac gatatccagc aagccgtcgc
     5461 cattgacatc ggcaacggcg atctggttgg cgtccactgc ggcgtagttg gccgctgcac
     5521 cgaatgtgcc ggggctgctc gcactgttga gcaataccgt gaccgcattg gatccgccga
     5581 tgatgatgtc gggacgacca tcgccattga tgtcaacgac ctggatcgac tgcgccagac
     5641 tgttcgcagc ggtcggatag ttgaccggtg ccagaaaggt ccccgggctc gccggattct
     5701 gcagcagcac cgcgacaaat ggcgcggcgc cgccgtacgg gcccgggtcg gtaatgatca
     5761 gatcgtcata accatcgccg ttgacgt
//
LOCUS       DATMRM010000073         6869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3129958, whole genome shotgun sequence.
ACCESSION   DATMRM010000073 DATMRM010000000
VERSION     DATMRM010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3129958"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VNZ06_02185"
     CDS             <1..448
                     /locus_tag="VNZ06_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19590.1"
                     /translation="TPDGVRLRPLVQMLSDALEAPTIVERERYLQRLGDVSLFISGFL
                     AHGFAAKLIDIDYHIAMGGQAYSTLANGLARGRRQVLSRVFAELAAKFQPIVDVLNEI
                     SDAARRYSQADILRLYEIWLKTGSNRARGLLRQLGVEASPVALLTQ"
     gene            453..1103
                     /locus_tag="VNZ06_02190"
     CDS             453..1103
                     /locus_tag="VNZ06_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19591.1"
                     /translation="MVLQPLQHLLSDIYDLPVQYRVDDFLFTERSALPAAVRESQTDE
                     QVLVLDEGDEAAVGLFLDPQLLARLAAANPVEALNSGNVADYWTALEGVSHFICLAWN
                     AAHDRAVSVLELELQAEIDKYVGSWWLLREQDPERYPAELHRLLFERTHIDSRLAGDR
                     AELYRQAHQYAARFCRRLERLLLAETVRRRHEALCELRRFYRLSRERKMTHIQRHG"
     gene            complement(1110..1376)
                     /locus_tag="VNZ06_02195"
     CDS             complement(1110..1376)
                     /locus_tag="VNZ06_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19592.1"
                     /translation="MSNRNSDPPDSSSDNEKSLYELSLEDTLAQKMRGVPVKKRVSAS
                     GYNPYDAMSAGKSQEPPRKPTDLRKLSEWIRLQRQVEELKKRDP"
     gene            complement(1382..1909)
                     /locus_tag="VNZ06_02200"
     CDS             complement(1382..1909)
                     /locus_tag="VNZ06_02200"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWW19593.1"
                     /translation="MSIEKSKTVFVGRIVTVNVETVRLPNGHVVDLDIIHHPGGAAIA
                     AVDSQRRVCLIHQYRHAAGGWIWELPAGKLEPQEPPEQTARRELVEEAGASAHSWTSL
                     GPYVSSPGVFTEIVHLYLARDLESVNMSHEVGELIEVHWIDLEEACTRALDGRISDGK
                     TALGLLRARTVLERS"
     gene            1929..2483
                     /locus_tag="VNZ06_02205"
     CDS             1929..2483
                     /locus_tag="VNZ06_02205"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWW19594.1"
                     /translation="MPHLLIVTGAPGSGKSRLAGALLDRVEARCCSKDEIKETLFDVL
                     GSADEAWSRRLSDASFALMFQFAQAWLTAQGLLILEGNFRAGEHEARLQALLDRTGAH
                     GAQVLCIAAPATRAERLAKRAADHTRHPAHQDNRISVQTGVGAAFLELPGPRFKFDSE
                     APWEREFTDLLLGLRNWYGLFKEL"
     gene            2532..4619
                     /locus_tag="VNZ06_02210"
     CDS             2532..4619
                     /locus_tag="VNZ06_02210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HWW19595.1"
                     /translation="MADASQFESLNAQQKRAATYGQVSGEKGWRSGPLLVVAGAGTGK
                     TSTIAHRVAQMVINGADPGRILLLTFTRRAAAEIRRRVHDIVRETLGDTLGNKAQAVL
                     QRLIWAGTFHSIGNRLLRHYARHLKLEPTFNVIDRSDSADLIDDLRTQLGLAVKEQRF
                     PRKDTCLMIYSMRVNTQRSLKETLEQQFPWCKSWEEDLTKLFRSYVEYKQRYGLLDYD
                     DLLLYWNLMMSDPKLAQHVSSNFDHVLVDEYQDTNKLQAEILHALKPDGAGVTVVGDD
                     AQAIYSFRGAAVENILKFPERFTPKAEIVTLAQNYRSTQSLLDVSNALMADAPRQHRK
                     HLLATRGGAGKPMYVTVDDLAGQAEYVCNQVLKRRETGVSLRSQAVLFRNASASDVLE
                     VELTRRKIPFVKYGGLKFMEAAHIKDLVALLRWADNPRNTLAAFRALQLLPGMGPVNA
                     RKIVDLIEQQGGSLDALGGFTPPQQMGPTYGKLQALMKALAEPQRPWPGQIRQVRDWY
                     QPHLERIYEHVHTRIGDLDQLEQLGTQFPSRERFLTELTLDPPNASSDLSSGASHDED
                     YLVLSTVHSAKGMEWDTVYLLNVVDGSFPNEFNTSRAEQLDEERRLLYVAMTRARNEL
                     QLCVPLKFPLTQQSRTGDAHVYGGRSRFVSEKVLKCVQQLAFQGAQLGDASPLASKNE
                     ATVDVTARLKDMW"
     gene            complement(4654..6657)
                     /gene="speA"
                     /locus_tag="VNZ06_02215"
     CDS             complement(4654..6657)
                     /gene="speA"
                     /locus_tag="VNZ06_02215"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HWW19596.1"
                     /translation="MVRPIEKAPPEGLEDIVGQAQAVRNASSSQAWGVEDALELYNVP
                     AWGLGYFSINSAGHLVVRPDTTPANEIDLLEVVQGLQERDLSAPVVIRFSDILAHRLR
                     HLHTAFAQAIAENDYRNRYTAVYPIKVNQQRLVVEEVFRYGKEFGFGLEAGSKPELLA
                     VMAMTGGADDRPIICNGFKDSSYIEAVILATKLGRTIIPVIENFAELALVLRHADTYK
                     VRPKLGVRVKLATEGSGRWRDSAGEKSKFGLFISEILELVSTLRARDMLDCLKLVHCH
                     PGSQLQDIRRVKDAINELAHVYGELKTMGAGLEYIDIGGGLGIDYDGSGTNYPSSMNY
                     TLNEYAGDVVYRIGSVCNARSIPHPMIISESGRAIAAHHSVLIFNVLGNSALDRFRID
                     AKDVTEFGGVKALPAPVRDLLDAYRNIDERRLVECYHDATTARDQALQMFNLGLLSLE
                     IRGFTERLYWASCARVRDVCRRLERAPEELDDLENVLSDTYFCNFSLFQSLPDSWAID
                     QLFPVMPIHRLADLPTRKAVLADITCDSDGKIDRFVGPRDVKRTLELHELVAGQDYYL
                     GAFLVGAYQETLGDLHNLFGDTHVVHIKLHPEGGWSIEEIVKGDTANKVLEYMEYDVA
                     ELTPALTRDCERAVREGRLTVAESQTLKRFYENELNGYAYLEL"
BASE COUNT         1322 a         2094 c         2137 g         1316 t
ORIGIN      
        1 gacgccagat ggagtgcgac tgcgtcctct ggtgcagatg ctgtccgacg cgctcgaagc
       61 accgacgatc gtggaacgcg agcggtacct gcagcgcctg ggcgatgtgt ccctgttcat
      121 ctccggattt ctggcgcacg gcttcgcggc caagctgatc gacatcgact atcacattgc
      181 gatgggcggc caggcttaca gcacactggc caacggcctg gcgcgtggac ggcgacaggt
      241 cttaagccgc gtattcgccg agctggcggc caagtttcag ccgatcgtgg acgtactcaa
      301 cgaaatcagc gacgccgcgc ggcgctactc gcaggccgac atcctgcggc tgtacgaaat
      361 ctggctcaag accggtagca accgcgcccg cgggctgctg cgtcagcttg gtgtggaagc
      421 ctcgccggtc gcgctgctga cgcaatagcc aaatggtatt gcagccgctg cagcacctgc
      481 tgtcggacat ctacgatctg ccagtgcagt atcgggtcga tgatttcctg ttcaccgaac
      541 gctctgcgct gcctgccgcc gtgcgagagt cgcagaccga cgaacaggtg ttggtcttgg
      601 acgagggcga tgaagcggcg gtgggcctgt ttctggatcc gcagctactg gcacgattgg
      661 ccgccgccaa tccggtcgaa gcgctcaact ccggcaacgt cgcggactat tggaccgcgc
      721 tcgagggtgt cagccatttc atctgtctgg cctggaatgc cgcccacgac cgggcggtgt
      781 ccgtgctcga actggagctg caggccgaga tcgacaagta cgtgggcagc tggtggctgc
      841 tgcgagagca ggatccagag cgctacccgg cggaactgca tcgcctgctg ttcgagcgta
      901 cgcacatcga tagccgtctg gcaggtgatc gagccgagct ttaccgccag gcgcaccagt
      961 acgctgcgag gttctgtcgg cggctggaac ggctgctatt ggccgaaacg gtgcggcggc
     1021 gacatgaggc gctgtgcgag ctgcgacgct tttatcgtct cagccgcgag cgcaaaatga
     1081 cgcacatcca gcgtcacggc tagcgcattc tacggatcgc gcttcttcag ctcttcgacc
     1141 tgacgctgca agcggatcca ttccgacagt ttgcgcagat cagtcggttt gcgcgggggc
     1201 tcctgcgact tgccagcgct catcgcgtcg tacgggttgt agccactggc tgagacgcgc
     1261 ttcttgaccg gtacgccgcg catcttctgg gcgagggtat cctcgagaga aagctcgtag
     1321 aggcttttct cgttatccga agacgagtcc ggcggatctg aattgcgatt actcacggcc
     1381 ctcacgaacg ctccagcact gtacgcgctc gcagcagtcc aagtgcggtc ttgccgtcgc
     1441 tgattctccc gtcaagcgcg cgtgtgcatg cctcttcaag atcaatccag tgaacctcaa
     1501 tgagttcacc gacctcgtgc gacatattga ctgattccaa atcgcgggct aggtacaagt
     1561 gcacgatttc ggtgaaaaca cctggtgagc tgacataagg gccgagactg gtccaggaat
     1621 gtgcgctagc tcccgcctct tcgaccagtt cgcggcgcgc cgtctgctcg ggcggctcct
     1681 gcggttcgag tttgccggca ggtaattccc agatccagcc gccggcagca tgacgatatt
     1741 gatggatcag acacacgcgc cgctgcgaat ccacggccgc gatcgccgcg cctccgggat
     1801 gatggataat gtccagatcc accacgtggc cgttgggaag ccggactgtc tcgacgttga
     1861 cggtgacgat gcgcccgacg aacacagttt tcgacttttc gatgctcatg tgccagagcg
     1921 taccgtaaat gccgcacctg ttgattgtga cgggcgcgcc gggcagcggt aagagccggc
     1981 tcgccggagc gcttctcgat cgggtcgaag cccgctgctg cagcaaggat gaaatcaagg
     2041 agaccctgtt cgatgtgctg ggcagcgcgg atgaggcctg gtcccggcgc ttgagcgatg
     2101 ccagtttcgc gttgatgttc cagtttgctc aggcgtggct gacagcccag gggctgctga
     2161 tattggaagg gaacttccgt gccggcgaac atgaggcccg gctgcaggca ctgcttgatc
     2221 gcaccggcgc ccatggcgcg caggtactgt gcattgcggc gcccgcaacc cgggccgagc
     2281 gtctggcgaa acgtgcggca gatcacactc gccatccggc tcaccaggac aaccgaatca
     2341 gcgtgcagac gggtgtcggt gcggcgttcc tcgaactacc gggcccgcgt ttcaagttcg
     2401 acagcgaggc gccctgggag cgcgagttca cagacttgct gcttggactg aggaattggt
     2461 acgggctgtt caaggaactt tgagacaccc gtctacacaa cgtcccgccg tgcattatat
     2521 ttcgagccga tatggccgac gcttcacagt tcgaaagtct caacgcccag caaaagcggg
     2581 ccgcaaccta tgggcaggta tccggtgaga agggctggcg ctccggtccg ctgttggtgg
     2641 tggccggcgc cggcaccggc aagaccagca ccatcgccca tcgcgtcgcc cagatggtta
     2701 tcaacggggc cgatccgggg cgcattctgc tgctgacgtt cactcgtcgt gccgcggccg
     2761 agatccggcg ccgggtacac gatatcgtgc gcgaaacctt aggggatacg ctcggcaaca
     2821 aagcccaggc ggtgctgcaa cggctcatct gggccggcac attccattcg atcggcaacc
     2881 ggctgctgcg gcattacgcc cggcacctga agcttgaacc cactttcaac gtcatcgatc
     2941 gctccgattc ggccgatctg atcgatgacc tgcgtaccca gctgggactg gcggtcaagg
     3001 agcagcgttt tccccgcaag gacacctgtc tgatgatcta ttcgatgcgg gtcaacacgc
     3061 agcgcagcct caaggagacc ctggagcagc agtttccgtg gtgcaagagc tgggaagagg
     3121 acctgaccaa gctgtttcgc agttacgtcg agtacaagca gcgctatggg ctgctcgatt
     3181 acgatgattt gctgctgtat tggaacctga tgatgagcga ccccaagctg gcgcagcatg
     3241 tcagcagcaa ttttgatcac gtgctggtcg atgagtatca agacaccaac aagctgcagg
     3301 cggagattct gcacgccttg aaacccgatg gcgcgggcgt caccgtggtc ggcgacgatg
     3361 cgcaggcgat ctattcgttc cgcggggcgg cggtcgaaaa catcctgaag tttcccgagc
     3421 gcttcacgcc caaggccgag atcgtgacgc tggctcagaa ctaccgctcg acccagagtc
     3481 tgctggacgt ttcgaatgcg ttgatggcgg acgctccacg ccagcatcgc aagcacctgc
     3541 tggcgacccg gggtggtgcc ggcaagccca tgtatgtgac ggtggacgat ctggcagggc
     3601 aggccgaata cgtttgcaac caggtactca agcggcgcga gactggtgtg tcgctgcgca
     3661 gccaggcggt cctgttccgc aacgccagcg ccagcgacgt gctcgaggtg gaactgactc
     3721 gccgcaagat tccgtttgtc aaatacggcg ggctcaagtt catggaggcg gcgcacatca
     3781 aggatctggt ggcgctgctg cgctgggccg acaatccgcg caacacgctg gcagcgttcc
     3841 gtgcgctgca gttgctgccc gggatggggc cggtgaatgc acgcaagatc gtcgatctga
     3901 tcgagcagca gggcggctcc ctcgatgcgc tgggcggatt cacgccaccg cagcagatgg
     3961 ggccgactta tggcaagttg caagcgctga tgaaggcgct ggcggagccc cagcgcccct
     4021 ggcccggcca gatccggcaa gtgcgtgact ggtatcagcc gcacctggag cgcatctacg
     4081 agcacgtgca cacgcgtatc ggcgatctgg atcagctcga acagctcggt acgcagttcc
     4141 cgtctcgcga acgcttcctg accgagctga cgctcgatcc acccaacgcg agcagcgatc
     4201 tgtcgagcgg cgcctcacat gacgaagact acctggtgct gtccacggtg cattcagcca
     4261 agggtatgga gtgggatacg gtctacctgc tgaatgtcgt ggacggcagt ttcccgaacg
     4321 aattcaacac cagccgcgcc gaacagctcg atgaagagcg ccggttgctc tatgtcgcga
     4381 tgacccgcgc tcgcaacgaa ctgcagttgt gtgtgccgct gaagttcccg ttgacccagc
     4441 agtccaggac cggcgatgcg cacgtctacg ggggtcgcag ccgcttcgtc agcgagaagg
     4501 tcttgaagtg cgtgcagcag ctggcgtttc agggggcgca gctgggtgat gcctcgccgc
     4561 tggcgtccaa gaacgaagcg accgtggacg tcaccgcacg cctcaaggac atgtggtagg
     4621 ccccgcgacc gcgtggtcgg gcgcagttcg cttctaaagc tccagatagg cgtagccgtt
     4681 cagctcgttc tcgtagaagc gcttcaacgt ctggctctca gcgaccgtta gccggccttc
     4741 gcgcaccgcg cgctcgcagt cgcgggtgag ggcaggggtt agctcggcga cgtcatactc
     4801 catatattcc agtactttgt tggccgtatc gcccttgacg atttcctcga tcgaccagcc
     4861 gccttccggg tgcagcttga tatgcaccac atgcgtatca ccgaacaggt tgtgcaggtc
     4921 acccaaagtc tcctggtagg caccgaccag aaaggcgccc aggtaataat cctggccggc
     4981 gaccagctcg tgcagctcca aggtgcgctt gacgtcgcgg gggccgacaa aacgatcgat
     5041 cttgccgtcc gagtcgcagg tgatgtctgc caggacggct ttgcgggtcg gcaggtccgc
     5101 gaggcgatgg atcggcatga ccggaaacag ctgatcgatc gcccaactgt cgggtagcga
     5161 ctggaacagt gaaaagttac agaaataagt atccgacagc acgttttcca agtcatcgag
     5221 ctcttccggg gcacgttcca gccgacggca gacgtcgcga acgcgtgcgc aactggccca
     5281 gtagagccgc tcggtgaatc cgcgaatctc gagcgacagc aatcctaggt tgaacatctg
     5341 cagcgcctga tcgcgcgcgg tagtggcatc gtggtagcac tccaccagcc gccgctcatc
     5401 gatgttgcga taggcgtcca acaggtcgcg caccggggcg ggcagagcct tgacgccacc
     5461 gaattcggtc acgtccttgg catcgatccg gaatcgatcc agcgccgagt tgccgagcac
     5521 attgaagatc aggacgctgt ggtgcgcggc aatcgcccgt ccggattcac tgatgatcat
     5581 cggatgcggg atgctgcgcg cgttacagac gctgccgatg cgatagacca catcaccggc
     5641 atactcattg agcgtgtagt tcatcgacga cgggtagttg gtgccgctgc cgtcgtagtc
     5701 gatcccgaga ccgccgccga tgtcgatata ttcaagaccg gcgcccatgg tcttcagctc
     5761 gccatacaca tgggcaagct cgttgatcgc gtccttgacc cgtcggatgt cctgcagctg
     5821 actgccaggg tgacagtgca ccagcttcag gcagtccagc atgtcacgcg cccgcagggt
     5881 cgagaccagc tcgagaatct ccgaaataaa aagtccaaat ttggactttt cgcccgctga
     5941 atcgcgccag cggcccgaac cttcggtggc gagctttacc cgcacgccca gcttcgggcg
     6001 caccttgtac gtgtcggcgt gtctcaggac caatgccagc tcagcgaaat tctcaatcac
     6061 cggaatgatc gtgcgaccga gcttggtggc cagaatcact gcctcgatat agctggagtc
     6121 cttgaagccg ttgcagatga tcggccggtc atcggccccg ccggtcatcg ccatgactgc
     6181 cagcagttca ggcttggagc cggcttccag gccaaagccg aattccttgc cgtaacggaa
     6241 cacttcttcg accacgaggc gctgctggtt gaccttgatc ggatagaccg cggtatagcg
     6301 attgcggtag tcgttttcgg caatcgcctg ggcaaaagcg gtgtgcaggt gccgcagccg
     6361 atgcgccagg atgtccgaga agcgaatcac gaccggcgcc gacaggtccc gctcctgcaa
     6421 accctgtacg acttcgagca gatcgatctc attggccggg gtcgtgtccg gtcgcacgac
     6481 cagatggccg gccgaattga tcgagaagta ccccagtccc caggccggaa cgttgtacag
     6541 ctcgagcgcg tcctccacac cccaagcctg gctactcgac gcgttccgca ccgcttgcgc
     6601 ttgacccacg atgtcctcga gcccttcagg gggcgccttt tcgatggggc gcaccataac
     6661 aaaacgccgc gacaagtaaa tgatcggcgt tctcaatgcg gtatctcgga agcagtgcca
     6721 atggctacaa ttgagctgtc atggccatcg atttctattg gggaagcggc agccctccgt
     6781 gctggagggt ggcgttggcg cttgaacaca agggcctgcc ctacaacagc catctgctgc
     6841 agttcgatcg ccaggagcac aagtcgccg
//
LOCUS       DATMRM010000074         7541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2201090, whole genome shotgun sequence.
ACCESSION   DATMRM010000074 DATMRM010000000
VERSION     DATMRM010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2201090"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VNZ06_02220"
     CDS             complement(<1..576)
                     /locus_tag="VNZ06_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19597.1"
                     /translation="MKTSSACISLGLFSLLGIGSALAASADTQTIATLSEIRDAAMSS
                     DWAWKRLEELTDRIGPRLSGSPQNAAAVAQVAAAMRALGADVRLQPVKVPHWVRGEEH
                     AELVDYPGRPSGVTQTLHLTALGGSSATPSSGLSARVIVVHDFDELKAHATEVRGSIV
                     LYEVKFDQRLADNGEAGEAYGQAGLYRFSGPA"
     gene            677..1891
                     /locus_tag="VNZ06_02225"
     CDS             677..1891
                     /locus_tag="VNZ06_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWW19598.1"
                     /translation="MSPVEPSSPSQRAKTWAAQHRLAAALACGVLAALLIVFGRAWHL
                     FGASVTPSPPAPVPVGAFRLSDEQLKSMTIEPILDQPFHSEEVTDGKIAYNADTITPV
                     YSPYSGRVTRLLAPLGSAVRQGQGLFELQASEFAQAESDLLTAAAQLKLTGATEERRH
                     AQYDAHAGSLQDWQQSQNELAGAQAALAAVRNRLRILGKSDSDIEAILSGGRPQAQVA
                     AVAPITGVVVDRQIGPGQFLTAGGATPVYTIADLSTVWLVANVREAQASRVHVGQSVA
                     VHVLAIPDREFDARLSYVAASVDPASRRLTVHAVLDNSEHLLKPEMFANFTIATSTDS
                     IAPAVPQEAVIFEGSQARVWVMQGARDASVRQVTLGRARRGKYEVLQGLKAGDRVVTR
                     GALFIDRAASGG"
     gene            1905..5069
                     /locus_tag="VNZ06_02230"
     CDS             1905..5069
                     /locus_tag="VNZ06_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HWW19599.1"
                     /translation="MNAIVAFALRQRFFMMVLLALVLLAGIVSFTRLNIEAYPDPVPP
                     LVDIVTQSSGQSAEEIERYITIPIEIQMAGIPNVTTVRTISLFGLSDVKVQFTYAYTY
                     SQAEQWVINRLSQLPPLPNNTQPQLSPESPIGEIYRYRVVGPPGYSVMDAKTLQDWVL
                     ERRFKAVPGVVDVTGWGGMTKAYEITIDQRRLAANALSLSQVLQVLGNSNLNVGGQTI
                     NIGPQSAVVRGVGLIHTTDDIRNTMLTSTHGTPVLVSDVASVNVSHLPRLGIAGQDAD
                     DDIVQGIVLMRRGAQSIPTIRAVEREVDRINASGILPPGVRIERIYDRSELIAVTTRT
                     VLHNMVLGIVLIFFIQWAFLGDLRSALIVATTIPFALSFAIGLLVLSGESANLLSVGA
                     IDFGLIVDASVIMVENIFRHLAESHEQRRAGSPMLQRARLRYEFSGKFAVIANAATEV
                     NQAILFAAAVIVAGFVPLFTLSGIEGHIFGPMARTYGYAIVGGLIATFTISPALSALM
                     LPNAIQERETFIVRWQRRLYDASIGYALKHHTVVLVGLGALLLLSWLTMRGLGLEFLP
                     KLEEGNLWIRAEMPSSISLEEGNGYVNRMRERIASYPEVETVISQHGRPDDGTDATGF
                     FNAEFFVPLKRATLWPRAVTKEKLTAQMNSDLSEQFPGVDFNFSQYIQDNVEEAASGV
                     KGENSVKVYGNELATLERTAERIKAVLASVPGVTDLAVFNSLGQPTVNITIDRARAAR
                     YNLAPGDVDTAIQAAIGGSSPGDLYEQGSDRHFPILVRYAPQFRDSIETLRRITIPAT
                     DASTSSIMQVPISDLAQVQLVSGASFIYRESQQRYIPIKFSVRGRDLGSAVLEAQRKV
                     AEQVTLPGGYRLEWVGEFGNLQDALQRLEIVVPLSLLLIVVLLYFKFGSLIDVALAAS
                     VIPMALIGGIFALFLSGTAFSVSAAIGFVALFGIATMDGIIVLSFYNQHVDAGMDRMN
                     AMLHTCRTQMRPVVMTCIVACVGLVPAAFSTGIGSQVQRPLALVVVGGMLLAPALILI
                     VLPVLILMFSRRRALQALPNGEAG"
     gene            5066..6532
                     /locus_tag="VNZ06_02235"
     CDS             5066..6532
                     /locus_tag="VNZ06_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWW19600.1"
                     /translation="MNPQPHRRDGLALIVVLLLGGCAVGPNFKPPPAPDVTQYASEPV
                     TDISAAPTPGGEAQHFNTGLEISQQWWRAFASADVDRLVGQALAANPDLQAAQAALRA
                     ALENVAAQRGTFFPTLAGSYGYSRQQDPVATLAPTLNSGIPIFSLNTASLNVAYSVDV
                     FGLYRRQVESLQASADAQRYQMQATYLTLVSNTITAAIMQAAAHTQLQATRELIRSGR
                     ESLEVLQQQYRLGAISMSQVRAQEAALAQQEALLPALAQLQSAQHDLLAQLTGKFPSQ
                     LPLDELDLSMLSLPQELPLTLPAKLVGQRPDIREAQAQLHAATADVGVAIANMLPDIS
                     LSAGLGSTAVALSQLNQPGTRFWNYGASLSQTLFAGGALLHKKRAAVALMDQAGAQYR
                     STVLQAFRQVADTLAALQADALTLQAQARAERAAADSLGLVKQNLQLGSASYLDLLTA
                     QGLYSQAVVALAQARAARLTDTVALFQALGGAWWKPMP"
     gene            6551..>7541
                     /locus_tag="VNZ06_02240"
     CDS             6551..>7541
                     /locus_tag="VNZ06_02240"
                     /inference="COORDINATES: protein motif:HMM:NF028267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HWW19601.1"
                     /translation="MSWQKRRIARDQRLSSARAIAKGKIVSASDATSLLEAVVRPGDR
                     VCLEGDNQKQADTLAKALAACDPAKLHDLHMLQSGIMLPSHLDVFERRIARRLDYSYS
                     GPQSARIARMLYAGQIELGAVHTYLELFARYFIDLTPDVALIAAVSADREGNLYTGAN
                     TEDTPTIVEATAFHDGIVIAQVNEILERVPRVDIPGDRVHFITRSPNPFFVEPLFTRD
                     PAAITESQILMAMMAIKGIYAEYGVERLNHGIGFGTAAIELILPTYGEALGLKGKIAR
                     RWALNPHPTLIPAIESGWVQQIHCFGSEVGMDAYIAARPDVFFTGADGSLRSNR"
BASE COUNT         1304 a         2447 c         2448 g         1342 t
ORIGIN      
        1 ggccggtccg gaaaaacgat acaggccggc ctgaccatag gcctcgcccg cctcaccgtt
       61 gtccgccagg cgctgatcaa acttcacctc atacagaacg atgctgccgc gaacctcggt
      121 ggcgtgcgcc ttgagctcat cgaagtcatg cacgacgatc actcgcgcgc tcaggcctga
      181 ggagggcgtg gccgaagagc cgccgagcgc cgtcagatgc agtgtctgtg tcaccccgga
      241 aggccggccg ggatagtcca ccagctccgc atgctcctcg ccgcgcaccc agtgcggcac
      301 tttcaccggt tgcagacgca cgtcagcgcc cagggctcgc atcgccgctg cgacctgtgc
      361 aaccgcggcg gcattctgcg gtgaacccga cagacgcggt ccgatgcgat cggtcagctc
      421 ttccagccgc ttccaggccc agtcactgct catcgctgca tcgcgaatct cagacaacgt
      481 cgcgatcgtc tgggtatcgg cagacgctgc gagtgcggaa ccgatgccga gcagtgaaaa
      541 caggcccagt gagatgcaag cagacgaagt tttcataatt ggaatggtac ctcatcaaga
      601 tgtcgtacag gcttgtaatg ggaaaggtta gcgaaagccg tggtgcgtaa gctcgcgacg
      661 acttcagtag ccgaccatgt caccagttga accctcatca ccgagtcagc gcgcgaagac
      721 gtgggctgcc caacatcgac tcgccgcagc tctggcgtgc ggcgtgcttg cggcactgct
      781 gatcgtgttc ggccgggcgt ggcacctgtt cggtgccagc gtcactccct caccgccagc
      841 accggtgccc gtcggtgcat tccgcctgag cgacgagcaa ctcaagtcga tgacgatcga
      901 gccgatcctc gatcagccgt tccactccga agaggtgacc gacggcaaga tcgcttacaa
      961 cgccgatacc atcacgcctg tgtattcgcc gtattccgga cgggtgacgc gactgctcgc
     1021 gccgctgggc tccgcggtca gacaggggca gggactgttt gagctgcagg cctctgagtt
     1081 tgcacaggcc gaaagcgatc tgctgaccgc ggccgctcag ctcaagctga ccggcgcgac
     1141 cgaagagcgt cgtcacgcgc aatacgatgc gcatgccgga tcgctgcagg actggcaaca
     1201 atcacagaac gagctggccg gtgcacaggc cgcactcgcc gcggtgcgta accgcttgcg
     1261 cattcttgga aagagcgaca gcgacatcga ggcgatactc tcaggcggcc gcccgcaggc
     1321 gcaggtcgcc gctgtcgctc cgatcaccgg cgtcgtggtg gatcggcaga tcggtccggg
     1381 tcagtttctc accgccggcg gcgccacccc cgtgtacacc atcgccgatc tttccacggt
     1441 gtggctggtg gcgaacgtgc gcgaggccca ggccagtcgc gtgcacgtgg ggcagagcgt
     1501 ggcggtgcac gtgctggcga tacccgatcg cgagttcgat gccaggctca gctacgtcgc
     1561 ggccagcgtg gatccggcca gccgccgcct caccgtgcat gcggtgctcg acaactccga
     1621 gcatctgctc aaacccgaga tgttcgccaa tttcacgatt gccaccagca ccgacagcat
     1681 cgcgccggcg gttccgcagg aggcggtgat cttcgagggc tcgcaggcgc gtgtgtgggt
     1741 catgcagggc gcgcgcgacg cgtcggtgcg ccaggtgacg ctcgggcgcg ctcggcgcgg
     1801 taagtatgaa gtgctgcagg gactgaaagc aggtgatcgc gtggtaaccc gcggcgcgct
     1861 gttcatcgat cgcgccgcca gcggcgggtg atctcacagc ctccatgaac gcgatcgtcg
     1921 ccttcgcgct cagacagcgc tttttcatga tggtgctgct ggcgctggtg ctgctggccg
     1981 gtatcgtcag cttcactcgg ctcaatatcg aggcctatcc ggatccggtg ccgccgctgg
     2041 tggacatcgt cacgcagtcc tccgggcaat cggccgagga gatcgagcgc tacatcacga
     2101 ttccgatcga gatccagatg gccgggatcc cgaacgtcac cacggtgcgc acgatctcgc
     2161 tgttcggact gtcggatgtg aaggtgcagt tcacctacgc ctatacctac agccaggccg
     2221 agcagtgggt gatcaatcgc ctgtcgcagc tgccgccgct gcccaacaat acccagccgc
     2281 agctgtcacc ggaaagcccg atcggggaga tctatcgcta ccgcgtggtc gggccgccgg
     2341 gctattcggt gatggatgcg aagacgctgc aggactgggt gctcgagcgg cggttcaagg
     2401 ccgtgcccgg cgtggtcgac gtgaccggct ggggcggcat gacgaaagcc tacgaaatca
     2461 cgatcgacca gcgccgcctc gccgccaacg cgctatcgct gtcacaggtg ctgcaggtgc
     2521 tcggcaacag caacctgaac gtgggcgggc agacgatcaa tatcggcccg cagtcagcgg
     2581 tggtgcgcgg cgtcggactg atccacacga cggatgacat ccgcaacacc atgctgacct
     2641 cgacgcacgg tacgccggtg ctggtcagcg atgtggccag cgtcaacgtc agtcatctgc
     2701 cgcggctggg catcgccggc caggacgctg acgacgatat cgtgcagggc atcgtgctga
     2761 tgcgccgcgg ggcgcagagc attcccacga tccgcgcagt cgagcgcgag gtcgatcgca
     2821 tcaatgcgtc cggcatactg ccgccgggcg tgcgcatcga gcgcatctac gaccgcagcg
     2881 agctgatcgc tgtcaccacg cgcacggtgc tgcacaacat ggtgctcggc atcgtactga
     2941 tcttcttcat ccagtgggcg ttccttggcg atctgcgcag cgcgctgatc gtcgccacca
     3001 cgattccctt tgcgctctcg tttgcgatcg ggctgctggt cttgagtggt gagtccgcca
     3061 acctgctgtc cgtgggtgcg atcgacttcg ggttgatcgt cgatgcctcg gtgatcatgg
     3121 tcgagaacat cttccggcac ctcgcggaga gtcacgagca gcgccgcgcc ggatcgccga
     3181 tgctgcagcg ggcacgactg cgctatgaat tcagcggcaa gttcgctgtc atcgccaatg
     3241 cggcgaccga agtcaatcag gcgatcctgt tcgccgccgc ggtcatcgtc gccggtttcg
     3301 taccgctgtt cacactcagc ggcatcgagg gacacatctt cggtccaatg gcccggacct
     3361 acggctacgc catcgtcggc ggactgattg ccaccttcac gatctccccg gccctgagcg
     3421 cgctgatgct gccaaatgcc atccaggagc gtgagacctt catcgtgcgc tggcagcggc
     3481 ggctgtacga cgcgtcgatc ggctatgcgc tcaagcacca cacggtggtg ctggtggggc
     3541 tgggcgccct gctgctgctg tcgtggttga cgatgcgcgg gctcggcctg gagttcctgc
     3601 cgaagctcga ggaggggaac ctgtggatcc gcgccgagat gccgtcatcg atttcactcg
     3661 aggaaggcaa cggttacgtc aaccgcatgc gcgagcgcat cgcctcctac ccggaagtcg
     3721 agacggtcat ctcacagcat ggccggccgg atgacggcac cgatgcgacc gggttcttca
     3781 acgccgagtt ctttgtaccg ctcaagcgcg ccacgctgtg gccacgcgcg gtcaccaagg
     3841 aaaagctgac cgcgcagatg aactctgatc tctcggagca gtttccgggc gtggacttca
     3901 atttctcgca gtacattcag gacaacgtcg aggaggccgc gtcgggcgtg aagggggaga
     3961 attccgtcaa ggtctatggc aacgagctcg cgacgctcga gcgtacggcc gagcgcatca
     4021 aggcggtgct cgcgagcgtg ccgggcgtca ccgatctggc ggtattcaat tcattgggcc
     4081 agccgaccgt caatatcacg atcgatcgcg cccgcgccgc gcgctacaac ctcgcacccg
     4141 gcgatgtgga caccgcgatc caggcggcga tcggtggctc cagtcccggc gacctgtatg
     4201 agcagggcag tgaccggcac tttccgatcc tggtgcgcta tgcgccgcag tttcgcgaca
     4261 gcatcgagac gctgcgacgg atcacgatac cggccacgga tgcgtccacc agttcgatca
     4321 tgcaggtgcc gatcagcgat ctggcgcagg tgcaactggt ctccggcgcc tcatttatct
     4381 atcgcgagag ccagcagcgc tacatcccga tcaagttcag cgtccgcggg cgcgatctgg
     4441 gcagcgctgt gctcgaggcg cagcgcaagg tggccgagca ggtgacattg cccggcggct
     4501 accggctcga gtgggtcggg gagttcggca acctgcagga tgcgctgcaa cggctcgaga
     4561 tcgtggtgcc actgagtctg ctgctgatcg tggtgctgtt gtacttcaaa ttcggctcgc
     4621 tgatcgacgt ggcactcgcg gcgagcgtca tcccgatggc cctcatcggc ggcatcttcg
     4681 cgctgtttct gagcggcact gccttcagtg tgtcagccgc gatcggcttc gttgcgctgt
     4741 tcggcattgc cacgatggac ggcatcatcg tgctgtcgtt ctataaccag catgtcgatg
     4801 cgggtatgga ccggatgaac gccatgctgc acacctgccg cacgcagatg cgcccggttg
     4861 tgatgacctg catcgtcgcc tgcgtgggac tggtgccggc ggcattctcc accggcatcg
     4921 gatcgcaggt gcagcgcccg ctggcgctcg tggtggtcgg cggcatgctg ctcgcgccgg
     4981 cgctgatcct gatcgtgttg ccggtcctca tcctgatgtt ttcccgacga cgggcgctgc
     5041 aggcgctgcc gaacggtgag gccggatgaa cccacagcct caccggcgtg acgggctcgc
     5101 actgatcgtg gtgctgctgc tgggtggctg cgccgtggga ccgaacttca agccgccgcc
     5161 cgcacccgat gtcacgcagt atgccagtga gcccgtcacc gatatcagcg ccgcgcccac
     5221 gcccggcggc gaggcgcagc acttcaacac cggactggaa atatcgcagc agtggtggcg
     5281 agcattcgcc tcagcggatg tggaccgcct ggtcgggcag gcgctggcgg ccaaccccga
     5341 tctgcaggcc gcacaggccg cactgcgcgc cgcgctggag aacgtcgcag ctcagcgcgg
     5401 cacgtttttt ccgacgctcg ccggctccta cggctatagc aggcagcagg atccggtcgc
     5461 aacgctggcg ccgacgctca actcgggcat cccgatcttc agtctgaaca ccgcctcgct
     5521 caatgtcgcc tacagcgtcg atgtgttcgg gctgtatcgc cggcaggtgg aatcgctcca
     5581 ggccagcgcg gatgcacaac gctaccagat gcaggccacc tatctgacgc tggtctccaa
     5641 cacgatcact gccgcgatca tgcaggccgc ggcgcacacc cagctgcagg cgacccgcga
     5701 gctgatccgc tcagggcgcg aatcgctcga ggtgctgcag cagcaatacc gtctgggcgc
     5761 gatctcgatg tcgcaggtgc gcgcacagga ggccgcgttg gcgcagcagg aggcgctgtt
     5821 gccggcgctg gcgcagctgc agagcgccca gcacgatctg ctggcgcagt tgaccggaaa
     5881 gttcccgagt cagttgccgc tggacgaact ggatctgtcg atgctcagcc tgccgcagga
     5941 gctgccactg acgctgccgg ctaagctggt cgggcagcgg cccgacatcc gcgaagccca
     6001 ggcgcagctg cacgcggcca cggccgatgt gggtgtggcc atcgccaaca tgctgccgga
     6061 tatttcgctc agtgccggtc tcggcagtac cgcggtggcg ctgtcgcagc tcaatcagcc
     6121 gggcacgcgt ttctggaact acggggcatc gctcagtcag accctgtttg ccggcggcgc
     6181 gctgctgcac aagaagcgcg cggcggtggc gctgatggat caggccggcg cgcagtatcg
     6241 cagcaccgtg ctgcaggcat tccggcaggt ggccgatacg ctggcggcgc tgcaggccga
     6301 tgcgctgacg ctgcaagcgc aggcgcgcgc cgagcgcgcg gcggccgatt ccctggggct
     6361 cgtcaaacaa aacctgcagc tcggatcggc cagctatctg gatctgctga ccgcgcaggg
     6421 tctgtacagc caagcggtcg tggcgctggc acaggcgcgc gcggcacgcc tgacggacac
     6481 cgtggctctg ttccaggcgc tcggcggcgc atggtggaag cccatgccgt agccctgtat
     6541 ggttgcgccg atgagttggc agaaacggcg tatcgcgcgc gatcaacgat tgagcagcgc
     6601 acgcgcaatc gcaaaaggaa aaatcgtcag cgccagcgat gccacctctc tgctcgaggc
     6661 cgtcgtgcgc ccgggcgatc gggtgtgcct ggagggcgac aaccagaagc aggccgacac
     6721 gctggcgaag gcgctcgcgg cctgcgatcc ggccaagctg catgatctgc acatgctgca
     6781 gtcgggcatc atgctgccct cgcatctgga tgtgttcgag cgtcgcatcg cgcgccggct
     6841 ggactactcc tattccgggc cgcagagcgc gcgcatcgcg cggatgctgt atgccggcca
     6901 gatcgaactc ggggccgtgc atacctatct ggaactgttc gcccgctact tcatcgatct
     6961 gacgccggat gtcgcgctga ttgctgcggt cagtgccgac cgcgaaggca atctgtatac
     7021 cggcgcgaac accgaggata ccccgacgat cgtcgaggcc acggcctttc acgatggcat
     7081 cgtgattgcg caggtcaatg aaattctcga acgcgtccca cgggtggaca tccccggcga
     7141 ccgcgtgcat ttcatcacgc gctcgcccaa tccgttcttc gtcgagccgc tgttcacccg
     7201 cgatccggct gcgatcaccg agagccagat cctgatggcg atgatggcga tcaagggcat
     7261 ctacgccgaa tacggcgtcg agcgattgaa ccacggcatc ggcttcggca ccgccgcgat
     7321 cgagctgatt ttaccgacct atggtgaagc gctgggtctg aagggaaaga tcgcgcgtcg
     7381 ctgggcgctc aatccgcatc cgaccctgat tccggcgatc gagtccggct gggtgcagca
     7441 gatccactgc ttcggttccg aagtcggcat ggacgcgtat atcgcggcgc gtccggatgt
     7501 gttcttcacc ggcgccgacg gttcgctgcg ctccaaccgc g
//
LOCUS       DATMRM010000075         3094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_664279, whole genome shotgun sequence.
ACCESSION   DATMRM010000075 DATMRM010000000
VERSION     DATMRM010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664279"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..944)
                     /gene="xerD"
                     /locus_tag="VNZ06_02245"
     CDS             complement(36..944)
                     /gene="xerD"
                     /locus_tag="VNZ06_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HWW19602.1"
                     /translation="MEKATTDTTDPLIARFLDAVWMERGLSSNTLAAYRADLVALHRW
                     LSERPLTLAHCGRAELMDFIAARVKGGSRPRSTARQLSSFRRFFRYLVREGIVREDPT
                     AQIAMPKIGRSLPRSLSEAEVESLLGAPAVADPLGYRDRTMLEVLYATGLRVSELVSL
                     KLGQINLNQGVVRVLGKGNRERLIPLGEEAVRWMKAFIGGARNDILLDRSTDFLFPTR
                     RGDRMTRQAFWHIIKRYARKAGVDRELSPHTLRHAFATHLLNHGADLRVVQMLLGHSD
                     VSTTQIYTHVARERLKDVHSKHHPRG"
     gene            1093..1881
                     /locus_tag="VNZ06_02250"
     CDS             1093..1881
                     /locus_tag="VNZ06_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWW19603.1"
                     /translation="MTNANSAAHPDPADTQKLTLKRLAYGVYAWIEFTLMALLGVSIL
                     LILPGLMRRRRFVRRLARFALRLAGMPLTLTGAVDLPTPCILVANHCSYIDGVVLTAT
                     LPPSFSFVIKREMSHFPLAGTLLRRIGSEFVERRDRRVGARDTRRLLRSAASGNALAF
                     FPEGTFSEEVGLLKFHIGAFAAAARARLPVVPVVIRGTRRSLPAGSAWPNPGRIHVEI
                     LGVVPAMQSGESAEDGDLAIRLRDMARGRILSALNEPDLATGQA"
     gene            1878..2984
                     /gene="zapE"
                     /locus_tag="VNZ06_02255"
     CDS             1878..2984
                     /gene="zapE"
                     /locus_tag="VNZ06_02255"
                     /inference="COORDINATES: protein motif:HMM:NF015902.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HWW19604.1"
                     /translation="MSQGLRERYERELAARHYAPDPAQCAVVDRLDALRTRLLAENQP
                     GLIGRWWQRIRPRHNGARDTRGIYLWGGVGRGKTWLMDLFYASVPIRTRRRSHFHHFM
                     RDIHAQLRTLRGRADPLELAARRMARGTRLLCLDELFVSDIADAMLLGGLFEALLRYG
                     VMLVITSNSPPGELYRDGLQRARFLPAIDLLERELEVIGLEGAVDYRLRQLRRQPIYL
                     DSHAPTTATQLQNLFEQLAPEHGDAGTNLKVSGRSLHALRRRGDVVWFNFVTLCEGPR
                     SQEDYVEIAQEYRTVLLSDVPMFSRPQQDDAARRFIALLDEFYDQGVKLILSAEAAPE
                     GLYRAERLQFEFRRAASRLVEMQTEDYLARPHRT"
BASE COUNT          539 a          983 c          991 g          581 t
ORIGIN      
        1 cgggcctttc ttcatgcgtc attccgggct ggcgatcagc cgcggggatg atgcttggag
       61 tgcacgtcct tgagacgttc gcgcgccaca tgggtgtaga tctgcgtggt ggacacgtcg
      121 ctgtggccca gcagcatctg caccacgcgc agatcggcgc catggttcag cagatgcgtc
      181 gcaaacgcat gccgcagcgt atgtggcgag agctcacggt ccactccggc cttacgggca
      241 taacgcttga tgatgtgcca gaaggcctgg cgagtcatgc gatcgccgcg acgggtcggg
      301 aacaggaaat cggtacttcg gtccaacaga atgtcgttgc gtgcaccgcc gatgaaggcc
      361 ttcatccagc gcacggcttc ttcgcccagc gggatgagcc gctcgcggtt gcctttgccc
      421 agcacgcgca ccacgccctg attcaggttg atctggccga gcttgaggct gaccagttcc
      481 gagacccgca agccggtggc gtacaacact tccagcatgg tgcggtcccg gtagccgagc
      541 gggtcggcaa cggccggcgc acccagcaac gattcgactt ctgcctcgct cagcgagcgg
      601 ggcagcgagc gcccgatctt gggcatcgca atctgtgcgg tcggatcttc gcgtacgatg
      661 ccttcacgca ccaggtaacg gaagaatcgg cggaatgagg atagctgacg cgcggtcgaa
      721 cgcggacgcg aaccgccctt gacgcgcgcg gcaatgaagt ccatgagctc ggcgcgaccg
      781 cagtgcgcga gtgtcagtgg ccgttcgctg agccatcggt gtagcgcgac caagtccgcg
      841 cgatatgccg ccaacgtgtt cgacgacagg ccccgctcca tccataccgc gtcgagaaaa
      901 cgcgcaatga gcggatccgt cgtgtcagtt gtggccttct ccactgtcgc tgtagcctga
      961 ttagtcaatg cactgcccta atggttcatc tggctgcagc agtatgctga gcataggctg
     1021 ctgttcggtg tgatgatcct cacagccgga catgaccgtt aacataaaga gaactgcatc
     1081 acacacaagg ccatgacgaa tgcgaactcg gccgcccatc cggacccggc ggacacccaa
     1141 aaactaactc tcaagcgcct ggcgtacgga gtttatgcct ggattgaatt caccctgatg
     1201 gctctgctgg gggtgtcgat cctgcttatt ctgcccggac tcatgcggcg gcggcgattt
     1261 gtgcgccggc tcgctcgctt cgcgctgcgc ctggcaggca tgcccctgac gctgacggga
     1321 gccgtcgatc tgccaacccc ctgcatcctg gtggccaacc attgcagcta catcgacggt
     1381 gtcgtcctga ccgccacctt accgcccagt ttcagcttcg tgatcaagcg ggaaatgtcg
     1441 cactttccgc tggccggaac gctgctgcgc cggatcggct cggagttcgt cgagcgccgc
     1501 gatcgcaggg tcggtgcgcg cgatacgcgc cgtctgctgc gcagcgccgc gagcggcaac
     1561 gctctggcct tcttcccgga gggcaccttc agcgaggagg tcggcctgct gaaatttcac
     1621 atcggggcgt tcgccgccgc cgcgcgcgcg cgcttgccgg tcgtgcccgt ggtcatccgc
     1681 ggtacccggc gcagtctccc ggccggcagc gcctggccca atccgggccg cattcacgtc
     1741 gaaatcctgg gggtggtgcc ggccatgcaa tccggtgaat cggccgagga cggcgatctc
     1801 gccatccggc tgcgcgatat ggccaggggc cggatcctga gcgcgctgaa cgagcccgat
     1861 ctcgcgactg gccaggcatg agccaggggc ttcgcgagcg ctatgagcgc gagctcgccg
     1921 cgcgacacta cgcgcccgac ccggcccagt gcgcggtggt agaccggctg gatgcgctgc
     1981 gcacccggct gctggccgag aatcagccgg gtctgatcgg ccgctggtgg cagcgaatcc
     2041 ggccgcgaca caacggcgcg cgcgacaccc gcggaatcta tctgtggggc ggcgtcggcc
     2101 gtggaaagac ctggctgatg gacctgttct acgccagtgt gccgatccgc acccgccgcc
     2161 gcagtcactt tcatcatttc atgcgcgaca tccacgccca gcttcggacg ctgcgcgggc
     2221 gtgccgatcc gctggaattg gccgcgcgcc gcatggcgcg cggcacccgc ttgttgtgcc
     2281 tggatgagct gtttgtcagc gatatcgccg atgccatgct gctgggaggc ctgttcgagg
     2341 cgctcttgcg ctatggggtg atgctggtga tcacatccaa ctcgccgccg ggcgagctgt
     2401 accgcgacgg gctgcagcgc gcccggttcc tgccggcgat cgatctgctg gagcgggagc
     2461 tggaggtgat cggcctggaa ggtgcggtgg actaccgcct gcgccagctt cggcggcaac
     2521 cgatctacct ggacagccac gcccccacga ccgccacgca gctacaaaac ttatttgagc
     2581 agctcgcacc cgaacatggg gatgccggca ctaacctgaa ggtcagcggg cgttctttgc
     2641 atgcgctgcg ccggcggggc gatgtggtct ggttcaattt cgtcacgctg tgtgaggggc
     2701 ctcgtagtca ggaagactat gtcgagatcg cgcaagagta tcgcaccgta ttactgtcgg
     2761 atgtgccgat gttttccaga ccgcagcagg atgatgcggc ccgtcgattc atcgcactgc
     2821 tggacgaatt ttatgaccag ggggtcaagt tgattctgtc ggccgaagct gcaccggaag
     2881 ggctctaccg cgcggaacgc ctgcagttcg agttcaggcg tgccgcaagt cggctggtgg
     2941 aaatgcaaac tgaagactat ctggcacgac ctcatcgtac ctaacccaac atcctaattg
     3001 acatggctac aaatccatca ttcatgcaag gtccattgga actgcgggtg gccgagatcg
     3061 aaggcgccat cgcacagatt gccggtcgct ttcc
//
LOCUS       DATMRM010000076        11502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3755589, whole genome shotgun sequence.
ACCESSION   DATMRM010000076 DATMRM010000000
VERSION     DATMRM010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11502
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3755589"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /gene="eno"
                     /locus_tag="VNZ06_02260"
     CDS             <1..350
                     /gene="eno"
                     /locus_tag="VNZ06_02260"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HWW19605.1"
                     /translation="LFVTNTKILAEGISKGIANAILIKLNQIGTLTETLAAIDMAEKA
                     RYASIVSHRSGETEDCTIADVAVATAATQIKTGSMSRSDRVAKYNQLLRIEEQLGGKP
                     RYAGREAFPVVLT"
     gene            358..702
                     /gene="ftsB"
                     /locus_tag="VNZ06_02265"
     CDS             358..702
                     /gene="ftsB"
                     /locus_tag="VNZ06_02265"
                     /inference="COORDINATES: protein motif:HMM:NF002058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HWW19606.1"
                     /translation="MKWLLVALAVALLLLQYRMFLSNDGIQEVLRLQDAVEAQKAQNA
                     RLAERNRQLAAEVHDLKQGYAALEERARTELGMIAGNESFYQVVPQKAGTGPAPAPHP
                     PPRDVQRTALAR"
     gene            992..1684
                     /gene="ispD"
                     /locus_tag="VNZ06_02270"
     CDS             992..1684
                     /gene="ispD"
                     /locus_tag="VNZ06_02270"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HWW19607.1"
                     /translation="MPAAGAGRRFGSAKQYAPLRERTVLEVSLQPFLDDAQCAGGSLV
                     LAADDPQRERLQRVLPGKMQLIDGGEERVHSVRNGLQALSQRAAAQDWVLVHDAARPM
                     LSAGDLAALLQQGGADAVGALLAVPVADTVKRARSGQTAVPSGAVRCETTMAREGLWL
                     AQTPQMFRFAPLCEALERALSVGRRPTDEAQAMEWQGHAALLVASRDPNLKVTTRADL
                     MLAEAILQQRAT"
     gene            1685..2167
                     /gene="ispF"
                     /locus_tag="VNZ06_02275"
     CDS             1685..2167
                     /gene="ispF"
                     /locus_tag="VNZ06_02275"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HWW19608.1"
                     /translation="MRVGSGIDVHAFGPGDSVVLGGVRIAHTRGVLAHSDGDVLLHAL
                     VDALLGAAALGDIGQHFPDSDPQWKGAHSQRFVLAAVDMLSRRSLRVVNADLTLVAQA
                     PKISLWRDEIRRSIALMLALPEACVNLKATTTEHLGFIGRNEGLMALATVLIDDVKAD
                     "
     gene            complement(2154..2702)
                     /locus_tag="VNZ06_02280"
     CDS             complement(2154..2702)
                     /locus_tag="VNZ06_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HWW19609.1"
                     /translation="MIAEDDEPEEDDRTLFRRAMRDVVPLKRSQRALPTHRKGRGRAT
                     FARAERAAVLRESLGIGADLYEVQPGDELIFRRAGVPQTVLRRLRRGQYRIEAEIDLH
                     GMTVSEASAQLGDFLRAARVNGLRCVRIIHGKGLRSGNRGPVLKNTVNELLRRSDPVL
                     AFASARPVAGGTGATLVLISRL"
     gene            2756..3508
                     /gene="surE"
                     /locus_tag="VNZ06_02285"
     CDS             2756..3508
                     /gene="surE"
                     /locus_tag="VNZ06_02285"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HWW19610.1"
                     /translation="MKILVTNDDGYRAEGITQLRAAMSTLGEVTVVAPDRNRSGAGNS
                     LTLEVPLRLSESEPGVHYVAGSPTDCVHLAISGVFDFEFDMVASGINDGPNLGDDVLY
                     SGTVAGAIEGRFLGLPTIAVSLCTRAGGPRHFDTAARVARRLVEALLARPLDAALILN
                     VNVPDRAYAELAGFASTRLGYRHRSERVVPAHDPRGRPVYWIGAAGASADAGAGTDFM
                     AVAQGFVSVTPLSIDLTRHAALPVVSQWLAAL"
     gene            3516..4181
                     /locus_tag="VNZ06_02290"
     CDS             3516..4181
                     /locus_tag="VNZ06_02290"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003222172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HWW19611.1"
                     /translation="MISDVRLAGIGMTSARTRDRLVQRLRDQGISNLAVLDRVRNVPR
                     HIFVDEAIASRAYEDTALPIGFGQTISQPYIVARMTEALLEGGPLGKVLEIGTGCGYQ
                     TAVLAPLVGRIFSVERIEALFKRARARLKELDIRNARLKHGDGIKGWKTQAPFDGVLI
                     AAAPLTIPEGLVEQLAIGGRLVVPVGPEGEQKLMRLTRREQGVERRVLGSVSFVPMLG
                     GVT"
     gene            complement(4504..4872)
                     /locus_tag="VNZ06_02295"
     CDS             complement(4504..4872)
                     /locus_tag="VNZ06_02295"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HWW19612.1"
                     /translation="MRLALESDPSIHLVRSYGEGVITVGAQELRGPCVIAPHGLLTEW
                     SATSVDELTEPQFAMLLTMPANIVLLGTDAAAQWPPAAIRAWCHSKGIALETMNLGAA
                     CRTYNILASEQRAVVAGLFP"
     gene            4893..5882
                     /locus_tag="VNZ06_02300"
     CDS             4893..5882
                     /locus_tag="VNZ06_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19613.1"
                     /translation="MIELVLVVTDYAPPEERSSGAQRPRLPLLERLLSRARSSALGAD
                     WRGWLAASAAPAGLAGFSPAAIAGASFRGSGVPKPESTGYWLATPVHFFAGLDSVHLH
                     PEGLLSLTAEEQAHLVRDFGRVFADSPWRLEAIGHRELLLSGPPLQADGADPARSLGD
                     DPSAGLARGDGAPALRRLGSEIEMWLYEHPVNAARLSRHELPVTALWLWGGQPPALPA
                     GVGRLVNPQLFGVDIYAQSLWQLQGLRALALSAASRAIGPEPMSLSCDSVVLLTNGLQ
                     ELEQHWLPGAQRALRQRRLSALHLILGEHLFRLSSLHWLRFWRSPIPWWEALV"
     gene            5879..7609
                     /gene="recJ"
                     /locus_tag="VNZ06_02305"
     CDS             5879..7609
                     /gene="recJ"
                     /locus_tag="VNZ06_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HWW19614.1"
                     /translation="MSLTIARRALSASPCAFDAPMHPVLQRVYAARGVHATAELSASI
                     RGLLPIGTLASVEAAAQLLWDHRQQGGLVLIIGDFDADGATSTALLVRALRSWGFGAV
                     DFLVPNRFEFGYGLTAEIVALAQQRQPSLIVTVDNGIASNAGVAAARAAGIDVLITDH
                     HLAGAELPQANVIVNPNLPGSSFASRALAGVGVAFYVAAALRRLLEQHAALPAGALAT
                     VELLDLVALGTVADVVSFDANNRILVSQGLARIRAGRCVPGISALLKLAERSQGTLIA
                     SDLAFAVAPRLNAAGRLSDMSIGIRCLLAQEEKQALMLAQELDSLNAERRQIEAKMQT
                     EALQAIRVLRDPGSGVTRAGVCLYDADWHQGVVGLVASRVKERLRRPVIAFARVDALT
                     LRGSARSIPGIHVRDVLDTIAVRDARLIQKFGGHAMAAGVTLEAQYLDRFAQAFDAEC
                     GRALARLGSPDVIETDGELTLEEIALTTALALRAGGPWGPGFPEPRFDGVFNICSSRM
                     VGERHLKLGLNAPEGRGQFDAIAFNYAEANSAPVLPLNGPARLVYRLETNEYQGEQRL
                     QFVIEHLLPV"
     gene            7721..8734
                     /gene="prfB"
                     /locus_tag="VNZ06_02310"
                     /pseudo
     CDS             7721..8734
                     /gene="prfB"
                     /locus_tag="VNZ06_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            8731..10236
                     /gene="lysS"
                     /locus_tag="VNZ06_02315"
     CDS             8731..10236
                     /gene="lysS"
                     /locus_tag="VNZ06_02315"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HWW19615.1"
                     /translation="MKDDHHGGEAPDEHRLIAERRAKLAAWRERGHAYPNDFRRDALA
                     AQLLGAYHERDAAWLEANPVRVHVGGRMMVKRTMGKASFAKIADRSGQIQLFLQQATL
                     GPVYDEFKGFDVGDFVGAEGVLFRTRTDELSVRVEKLVLLAKALQPLPDKWHGLTDTE
                     LRYRRRYVDLVMNDASRQVFRTRTRILRYLRDYLDALDFMEVETPMMQPIPGGAAARP
                     FRTHHNALDQDMYLRIAPELYLKRLIVGGFERVYEINRNFRNEGLSTQHNPEFTMLEL
                     YWAYTDYHELMELIERLLHGLADSLFASRLITYQGKQYDLGSPFRRVTVEEMILEHNP
                     VLERSHLRDVPYLRAACDRLGIAYKESDGAGKLQIEIFEKTGEHTLSDPTFVYAYPAE
                     VSPLSRRNDADAFLTDRFEFFLAGRELANGFSELNDPEDQAERFREQTERKDSGDDEA
                     MHYDADYVRALEYGMPPTAGLGVGVDRLVMFFTDSPSIRDVLLFPHLRAEG"
     gene            10250..11083
                     /gene="murI"
                     /locus_tag="VNZ06_02320"
     CDS             10250..11083
                     /gene="murI"
                     /locus_tag="VNZ06_02320"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HWW19616.1"
                     /translation="MDSASRPIGVFDSGIGGLTVLSALCAELPHEQFIYLGDTARLPY
                     GTKSAATIERYALQVADLLVARGVKALIVACNTASATALPAIRERYAQMPLIGVVEPG
                     AAAAIATSRNGRIAVIATEATVRGGAYQRAIAQRAPAASVSAVACQLLVALAEEGWTE
                     GVVARATAAQYLAPLFSAQRTSRPDTLVLGCTHFPMLRTVIGETVGAQVEIVDSAATT
                     ARAARELLQRERLLRPKPDAPAGNPLLLATDGVQRFASVGSRFLGKPIAAAEVELVDI
                     S"
     gene            complement(11122..>11502)
                     /locus_tag="VNZ06_02325"
     CDS             complement(11122..>11502)
                     /locus_tag="VNZ06_02325"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012677.4"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWW19617.1"
                     /translation="ASALARALLGLGATLITGGTENHLMVVDVMKSYGLTGAEAQTRL
                     EAAGIVCNKQVIPDDTLPPMRTSGIRLGTPAATTRGMKESQMQQAALLIDRVLRASAT
                     AADARAVLDSVRELIRAYPVPTLI"
BASE COUNT         2023 a         3480 c         3855 g         2144 t
ORIGIN      
        1 atctgttcgt gaccaacacc aagatcctgg ccgaaggcat ttcgaaaggc atcgccaatg
       61 cgattctgat caaactgaat cagatcggta cgctcactga aacactggcg gcgatcgaca
      121 tggccgagaa ggcgcgctac gcctcgatcg tctcgcaccg ttcaggcgag accgaagact
      181 gcaccattgc cgacgtcgca gtggcaaccg ccgccaccca gatcaagacc ggctcgatgt
      241 cgcgctcgga tcgcgtcgcc aagtacaacc aattgctgcg catcgaggag cagctcggcg
      301 ggaaaccgcg ctatgcggga cgcgaggcat ttcctgtcgt gctaacctaa cgccgccatg
      361 aaatggctgc tcgttgctct agcggtcgcg ttactgttgt tgcagtacag gatgtttctg
      421 tcgaacgacg gcatccagga agtgttgcgc ctgcaggacg cggtcgaagc acagaaggcg
      481 cagaacgcgc ggctcgccga gcgcaaccgt cagctcgccg cggaggtgca tgacctgaag
      541 cagggctacg cggcgctcga ggagcgagcg cgcaccgaac tcggcatgat tgccggcaat
      601 gaatcattct atcaggtggt gccgcagaag gcgggcacgg ggcccgcgcc tgcaccgcac
      661 ccgccgccgc gcgacgtgca gcgaaccgcc ctggctcgct aggaggctgt ctgagtaatc
      721 cacgtggccg cgaccgcggc agattttgct tcaggctagg cggagccgac gcagttgggt
      781 tcagacccaa taaggaggcg acaacgccgc ctgaagcaaa atccgcccga tcccgcaggg
      841 ttggctctgg caagggcgtc tgcggcgttg cttggcttgc ccatcgtgtc gactatgggc
      901 tgcgccgcgc gcctagcatc cgcctcttgc cagagccaac gcggctcacg cggattactc
      961 agacagcctg ctaatgcgct actggcttgt gatgcctgcc gcgggtgccg gtcgccgctt
     1021 tggcagcgcc aagcaatatg ctccgctgcg cgagcgaacg gtgctcgagg tttcactgca
     1081 gcctttcctc gatgacgcgc aatgtgccgg cggtagcctg gtgctcgctg ccgacgatcc
     1141 gcagcgtgag cgcttgcagc gggtgctgcc cggaaaaatg cagctcattg acgggggcga
     1201 ggaacgagtg cattcggtgc gcaatggctt gcaggcgctt agccagcgag cggcagcgca
     1261 ggattgggtg ttggtgcatg atgccgcccg accgatgctc agcgccggcg atctggcggc
     1321 actgctgcag caggggggtg ccgacgcggt cggagcgctg ctcgccgtgc cggtggccga
     1381 taccgtcaag cgcgcgcgca gcgggcagac ggcggtgccc tccggcgctg tgcgctgtga
     1441 aacgacgatg gcgcgtgagg ggctgtggct ggcgcaaacg ccacagatgt ttcgattcgc
     1501 gccgttgtgc gaggcactgg aacgggcgct gagcgttgga cgccggccca cggatgaagc
     1561 gcaggcgatg gaatggcagg gacatgccgc gctgctggtg gcttcccgtg atcccaacct
     1621 caaggtcacg accagggcgg acttgatgct cgccgaggcg attctgcaac agagggctac
     1681 ctaaatgaga gtcggatcag ggatagatgt gcatgccttc gggcccggcg actcggtggt
     1741 actgggtggg gtacgcatcg cgcacacgcg cggtgtgctt gcgcattcgg atggtgacgt
     1801 gctgctgcac gcactggtcg atgcgctgct cggtgcggcc gcactgggcg acatcggaca
     1861 gcatttcccg gacagcgatc cgcagtggaa gggcgctcac agccagcgct tcgtgctcgc
     1921 tgccgtcgat atgctcagtc ggcgaagtct gcgggtggtg aatgcggacc tcacactggt
     1981 ggcgcaggcg ccgaagatca gtctgtggcg agacgaaatc cgtcgcagta tcgcgttgat
     2041 gctcgccctg ccagaggcct gcgtgaatct gaaagccact accaccgaac atctcggctt
     2101 catcggccgc aatgagggac tcatggcgct ggctaccgtg ctgatcgacg atgtcaaagc
     2161 cgactgatca gtaccagtgt cgctcccgtg ccgcccgcta ccggtcgggc cgatgcgaat
     2221 gcaagcaccg gatcagaccg gcgcaacagc tcattgacgg tgttcttcag taccgggccg
     2281 cggttacccg agcgcaatcc cttgccatga atgatgcgca cacagcgcaa accgttgacg
     2341 cgcgcggccc gcagaaaatc tccaagctgc gccgaggctt ccgacacggt catgccatga
     2401 aggtcgatct cagcttcgat gcggtattgg ccgcgccgca atcggcgcag cacggtctgc
     2461 ggaacgccgg cacgccggaa aatcagctca tcccccggct gcacctcata cagatcggcg
     2521 ccgatgccga ggctttcgcg caggaccgcc gcccgttcgg cgcgcgcgaa ggttgcccgg
     2581 ccgcggcctt tgcggtgcgt cggcagtgcc cgctgtgagc gtttcagcgg taccacatcc
     2641 cgcatggcgc gccggaacag cgtgcgatcg tcttcctcgg gttcgtcgtc ttccgcaatc
     2701 actattgcag tatattggcg agcctcatgg gcattggccg tttcgataaa tgtccgtgaa
     2761 aatactcgtc accaacgatg acggctatcg agccgaaggg atcacgcagc tacgcgccgc
     2821 gatgtccacg ctgggtgagg tgacggtggt cgccccggac cgcaatcgca gcggtgccgg
     2881 caattcgctg acgctggaag tgcctctgcg gctgtcggag tccgagcccg gcgtgcatta
     2941 cgtggccggc agtccgaccg attgcgtgca cctggcgatc tccggcgtgt tcgactttga
     3001 attcgacatg gtggcctcgg ggatcaacga cggcccgaat ctgggggacg acgtcctgta
     3061 ttccggcacg gtggccggcg ccatcgaggg acgtttcctg ggactgccga ccatcgcggt
     3121 ctccctgtgc acccgcgctg gcggtccacg acacttcgac accgcggcgc gggtggcgcg
     3181 caggctggtg gaggcgctgc tggcgcggcc gctcgatgcc gcgctgatcc tcaacgtcaa
     3241 tgtgccggac agggcgtacg ccgagctggc cggatttgcc agcacgcgcc tgggctatcg
     3301 ccatcgctcc gagcgcgtgg tgccggcgca cgatccgcga ggtcgcccgg tctactggat
     3361 cggcgcagcc ggggcgagtg ccgatgccgg tgccggcacc gatttcatgg ccgtggcgca
     3421 gggcttcgtg tccgtcacgc cgctgtcgat cgatctcacc cggcatgccg cgctgccggt
     3481 cgtgtcgcaa tggctggcgg cgttgtgacc ccgacatgat cagcgacgtg cgccttgccg
     3541 gcattgggat gacctcggcg cgcacccgcg accgactcgt gcagcgcctg cgcgatcagg
     3601 ggattagcaa cctggcggtg ctggatcgcg tgcgcaatgt tccgcgtcac atcttcgtcg
     3661 atgaggccat cgccagccgg gcctacgagg acacggcatt gccgataggg tttggtcaga
     3721 cgatctcgca gccctacatc gtggcgcgta tgaccgaggc gctgctggaa ggtgggcctc
     3781 tgggcaaggt gctggagatc ggaaccggat gcggctatca gaccgcggtg ctcgcaccgc
     3841 tggtaggccg tatattttcc gtcgagcgca tcgaagccct gttcaagcgc gcgcgtgcgc
     3901 gactgaagga actcgatatt cgcaatgcgc gcctgaagca tggcgatggc atcaagggct
     3961 ggaagaccca ggcgccgttc gacggcgttc tgatcgcagc cgcgccgctg acgatccccg
     4021 aagggttggt cgagcaactc gccatcggcg ggcgactggt ggtgccggtg gggccggaag
     4081 gggagcagaa gctcatgcgg ctcacacgac gagagcaggg cgttgaacgg cgggtacttg
     4141 ggtctgtctc cttcgtgccc atgttaggag gtgtcacgta atggtgctct agagtacctt
     4201 aagtgttagt cgtaaaatat tgatattaat cattgaatat tggcgcactt gtaaaatcaa
     4261 tgcgctgcag attaagatga attcactgtc tgcggtaagc gacataagtc aaatgcctgg
     4321 gaggcggacg cgaagaaggc acagggaaaa aaaggggaag accggataaa acaaaaataa
     4381 aaaccaagtc taattacgag aaaaataagg tttagcgcgg atacagttgc tcggatcctg
     4441 tcagcttcga gcgattgggg tagttgatcg atcttctctc tctgtgcctt cttcgggtgc
     4501 gctttaaggg aacaatcccg caaccaccgc acgctgctca ctggcgagga tgttgtaagt
     4561 tcgacatgcc gcgcctaagt tcatagtttc caaagcaatt cccttcgaat gacaccatgc
     4621 gcggatcgct gccggcggcc actgtgcggc cgcatccgtt cccagcagta cgatgttcgc
     4681 gggcatcgtc agcagcattg caaactgcgg ttccgtcagt tcgtcgactg aggtcgcact
     4741 ccactcggta agcagaccgt gcggagcgat cacacacggc cctcgcagct cctgcgcccc
     4801 gaccgtgatg acaccctcgc cgtagctgcg gaccaggtgg atgctcgggt cggattcgag
     4861 tgcgagacgc atgatcagta cgatacgcca tcgtgattga attggtgctc gtagtgaccg
     4921 actacgcgcc gcccgaggag cgctcctcgg gcgcgcagcg tccacgtctg cccctgctcg
     4981 agaggctgct gtcgcgagcg agatcctcgg cgctgggcgc cgactggcgt ggctggctgg
     5041 ctgccagcgc ggcaccggct ggactggccg gattcagtcc ggcagcgatc gctggcgcct
     5101 cgtttcgggg ttcgggcgta ccgaagccgg agagcacggg ctattggctg gcaaccccgg
     5161 tgcacttctt tgccggactg gacagtgtgc atcttcaccc cgagggactg ttgtcgctca
     5221 ccgccgagga gcaggcacac ctggtgaggg atttcggacg ggtgtttgcc gactcgccgt
     5281 ggcgactgga agcgattggc catcgtgagc tgctgttgtc ggggccaccg cttcaggccg
     5341 acggcgccga tcccgcccga tcactgggcg acgatccgag tgcgggacta gcgcgcggcg
     5401 atggcgcgcc cgctctgcgg cgtctgggat cggaaatcga aatgtggttg tacgagcatc
     5461 cggtcaatgc ggcacgtttg tcgcggcacg aattgcccgt gaccgcgctg tggctgtggg
     5521 gcggtcagcc gccggcgctg cccgctggcg tggggcggct cgtcaatccg caactcttcg
     5581 gcgtggacat ctacgcgcaa agtctctggc aattgcaggg gttgcgcgct ctggcactat
     5641 cagcggcttc gcgggcgatt gggcccgagc cgatgtcgct gtcctgcgat agcgtggtgc
     5701 tcttgacgaa cggacttcag gaattggaac agcactggtt accgggtgca cagagggcgc
     5761 tgcgtcagcg acgactgtcg gcgttgcatc tgatcctggg agaacatctg ttccgtctgt
     5821 cgtcgctgca ctggctgcgg ttctggcgtt ccccgattcc ttggtgggag gcgctggttt
     5881 gagcctcacg attgcacggc gcgcgctgtc ggcatcgcct tgcgcattcg atgctcccat
     5941 gcacccggtt ctgcaacgcg tgtacgcggc gcggggcgtg cacgcgacgg cggaactgag
     6001 tgcgtcgatc cgcgggctgc tgccgatcgg aacactcgcc tcggtcgagg cggcagcgca
     6061 actcctgtgg gatcaccggc agcagggtgg gctggtgctg atcatcggcg acttcgatgc
     6121 cgacggggct accagcacgg cgctgctggt gcgcgcgttg cgcagctggg ggtttggcgc
     6181 cgtggatttt ctggtgccca atcgcttcga gttcggctat ggattgactg cggagatcgt
     6241 ggcgctcgcc cagcagcgac agccgtcgct gatcgtcacg gtagacaacg ggatcgccag
     6301 taatgccggg gttgccgctg cgcgcgcggc cgggatcgac gtgctgatca ccgatcacca
     6361 tctggcaggc gcggagcttc cgcaggcaaa cgtgatcgtc aatcccaact tgcccggcag
     6421 ttccttcgcc agccgtgcgc tggcgggcgt aggggtggcc ttttatgtcg ccgcggcgct
     6481 gcgtcgtctg ctggagcagc acgccgcatt gccggccggc gcgctggcca cggtcgagct
     6541 gctcgacctg gtggcactgg gcaccgtcgc cgacgtcgta tccttcgatg ccaacaatcg
     6601 catcctggtc tctcaagggc tggcgcgcat tcgcgctggc cgctgcgtgc cgggaatcag
     6661 cgccttgctg aagctggccg agcgcagcca gggcacattg atcgcctcgg atctggcttt
     6721 tgccgtggcg ccgcggctga atgcggccgg tcgtctctcg gacatgtcga tcggtattcg
     6781 ctgtctgctg gcccaggaag agaagcaggc gctgatgctg gcgcaggagc tggactcgct
     6841 caacgccgag cgacgtcaga tcgaagccaa gatgcagacc gaggcgttgc aggcgatccg
     6901 tgtattgcgc gatcccggca gcggggttac gcgcgccggt gtatgcctgt acgacgctga
     6961 ttggcaccag ggagtggtcg gtctggtagc cagccgtgtg aaggaacggc tgcgccggcc
     7021 ggtcatcgct ttcgcaagag tggacgcgct gacgctgcgc ggctcagcgc gctcgattcc
     7081 aggcatccac gtccgagacg tgctcgacac gatcgcggta cgcgatgcgc gtcttattca
     7141 gaagttcggc ggacatgcga tggccgctgg tgtgacgctg gaggcgcagt acctggatcg
     7201 cttcgcgcag gccttcgacg ctgaatgtgg tcgcgccctc gcgcggctgg gctcgcccga
     7261 tgtgatcgag accgacggtg agctgaccct ggaagagatc gcgctgacga ccgcgctcgc
     7321 gctgcgtgcc ggaggtccgt gggggccggg cttccccgag ccgcgtttcg acggggtatt
     7381 caacatttgc tcatcgcgca tggttggcga gcggcatctg aaacttggat tgaatgcacc
     7441 cgaagggcgc ggtcagtttg atgccattgc cttcaactac gccgaggcga acagtgcgcc
     7501 ggtgcttccg ctcaacggcc cagcccggct ggtctatcgg ctggagacca acgaatacca
     7561 gggcgagcaa cgcctgcaat tcgtgatcga gcaccttctg cccgtctaaa gcgggctgcg
     7621 gcctgttaga attgatggat ggaagtcagt cagctcaaac gcttgctcaa agatctcgag
     7681 gaacgccttg tctccttgag ggggtttctt tgagtacgac cgcaagaaag aacgtctaga
     7741 agaagtcagc cgcgaattgg aggatcccgc cgtctggagt tcgcaggagc gcgctcagga
     7801 gctgggtcgt gagcgcgcgc gactggtcgc ggacctcgaa gcgatggacg gtatcagcac
     7861 cgcggtcagc gatgcggccg aactgctgga actggccgaa agcgagaacg atgagtcggc
     7921 ggcacgcgag atcgagcgcg aggccagtgc cctcgaagcc ggggtgcgca agctggaatt
     7981 caagcgcatg ttccgcggcg agatggattc gcatagcgcc ttcctggata ttcaggccgg
     8041 cgcgggcggc acggaagcgc aggactgggc gcagatgctg ctgcggatgt atttgcgctg
     8101 gggcgcggca cacggattcg gctgcgaagt gattgattcc agtccgggcg aagtcgcagg
     8161 catgaagagc gcgacgatcg aggttcaggg cgaatatgct tacggttggc tgcgcaccga
     8221 aaccggcgtg caccgtctgg tgcgcaagtc gccgtttgat tccggcaatc ggcggcatac
     8281 ttcgtttgcg tcggtgtttg tgtcgccgga ggtggacgat gacatcgaca tcgacatcaa
     8341 tccggccgat gtcaagatgg atgtctaccg ctccagtggc gctggcggcc agcacgtcaa
     8401 caagaccgag tccgcagtgc gcctgacgca tgagccgagc ggtattgtgg ttgcctgcca
     8461 gaacgagcgc agccagcaca agaaccgctc cacggctatg aaaatgctga aggccaagct
     8521 ctacgaactg gaagtcaaca agcgcaacgc cgcggcgcgc gtgctggaag actccaaatc
     8581 ggatgtcagc tggggacacc agatccgctc ctatgtgctc gatcaatcgc gcatcaagga
     8641 tctgcgtact gcggttgagg tcggcaacac cgcggcggta ctggatggcg atcttgatca
     8701 atttctcgaa gcgagcctga aggcgggact gtgaaggacg accaccacgg cggcgaagcc
     8761 ccggacgagc atcggctgat tgccgaacga cgcgccaagc tggctgcctg gcgcgaacgc
     8821 ggccatgcct atccgaacga tttccggcgt gatgcgctgg ccgctcagtt gctgggtgcg
     8881 taccacgagc gcgacgcggc ctggctggaa gccaatccgg tgcgtgtgca cgtcggtggg
     8941 cgcatgatgg tcaagcgtac gatgggcaag gccagtttcg ccaagattgc cgatcgaagt
     9001 ggccagatcc agttattcct gcagcaggcc acgctcggtc ctgtttatga cgagttcaag
     9061 ggcttcgacg tgggcgactt cgtgggcgct gagggggtgc tatttcgcac gcgcaccgac
     9121 gagctgtcgg tgcgggtgga gaagctggtg ctcctggcca aggcgttgca accgctgccc
     9181 gacaagtggc acgggttgac cgataccgag ctgcgctacc gccgtcgcta cgtggatctg
     9241 gtgatgaacg acgccagccg ccaagtgttc cggacgcgca cccgcatcct gcgctacctg
     9301 cgcgactatc tggatgcgct cgacttcatg gaagtcgaga cgccgatgat gcaaccgata
     9361 ccgggcgggg cggcggcgcg cccattccgc acgcaccaca atgccctcga tcaggatatg
     9421 tatctgcgca ttgcgccgga gctgtatctg aagcggctga tcgtcggtgg cttcgagcgc
     9481 gtctacgaga tcaaccgcaa ttttcgtaac gagggactgt ccacacagca caatcccgag
     9541 ttcacgatgc tggagctcta ttgggcttac accgactatc atgagctgat ggagttgatc
     9601 gaacggctgc tgcacggtct ggccgactcg ctgttcgctt cacggctgat cacctaccag
     9661 ggcaagcaat acgatctcgg ctcgcccttc cgccgcgtga cggtcgagga aatgatcctg
     9721 gaacacaatc cggtgctgga gcgctcgcac ctgcgtgacg tgccgtatct gcgcgcggcc
     9781 tgcgatcgcc ttggtattgc ctataaagag agtgacgggg ctggaaagct gcagatcgaa
     9841 atctttgaaa agaccggtga acacacgctc tcggatccga ccttcgtcta tgcctatccg
     9901 gccgaggtct cgccgttgtc tcggcgcaat gacgcggatg catttctcac cgatcgcttc
     9961 gagttttttc tggccgggcg ggaactggcg aacggcttct ccgaactcaa tgatccggag
    10021 gatcaggcag agcgctttcg cgagcagacc gagcgcaagg actcgggtga cgatgaggcc
    10081 atgcactacg acgcagacta tgtccgcgcg ctcgaatacg gcatgccgcc gacggcgggt
    10141 ctgggggttg gggtggatcg gctggtgatg ttcttcaccg actcaccttc gattcgcgac
    10201 gtgctgctgt ttccgcacct gcgtgcggaa ggctaaggct gtcagcccag tggattcggc
    10261 ttcgcgtccg atcggagtct tcgattctgg gatcggcggg ctgacggtgc tgtcggcgct
    10321 gtgcgccgaa ctgccgcacg agcagttcat ctatctgggt gacaccgcgc gcctgcctta
    10381 tggaaccaag agcgcggcga cgatcgagcg ctacgcattg caggtcgccg acctgctggt
    10441 ggcgcgggga gtgaaggccc tgatcgtcgc ctgtaacacc gcctcagcga ccgcgctgcc
    10501 cgcaatccgc gaacgctacg cgcaaatgcc gctgatcggt gtggtcgaac ccggcgcagc
    10561 ggccgccatt gccacgtcac gcaacggtcg tatcgccgtg atcgcaaccg aggcgaccgt
    10621 gcgcggtggc gcctatcagc gtgccatcgc gcaacgggct ccggcggcca gcgtgagtgc
    10681 cgtcgcatgc caactgttgg tggcgctggc cgaggagggc tggaccgagg gcgtggtcgc
    10741 acgtgcgacc gccgcgcaat atctggcgcc gttgttcagt gcgcagcgca cgtcccgtcc
    10801 cgataccctg gtgctcggct gcactcattt cccgatgctg cggaccgtga tcggtgagac
    10861 ggtcggtgct caggtcgaga tcgtggattc agcggcgacc accgcgcgcg cggcccgaga
    10921 gctgcttcag cgcgagcgac tgctgcggcc aaagccggac gcccctgctg gcaatccgct
    10981 gctgctcgcg accgatggcg tgcaacgctt tgcgagcgtt ggcagccgct tcctcggcaa
    11041 gccgatcgcg gcggccgagg tcgagctggt ggacatttcc taagaatgct cccgagcgct
    11101 acggcaactc agccgagagg gtcagattag ggtcggaacc gggtaggcgc ggatcagctc
    11161 ccgcacacta tccagcacag ctcgcgcatc cgccgcagtc gccgacgcgc gcagcacgcg
    11221 atcgatgaga agcgctgcct gttgcatctg cgactccttc atcccgcgcg tggtggcggc
    11281 gggcgtaccg agtcggatgc cgctggtacg catgggcgga agggtatcgt ccgggatgac
    11341 ctgtttgttg caaacgatgc ccgcggcctc aagccgcgtt tgcgcttcgg ctccggtcaa
    11401 accataggat ttcatcacat cgacaaccat caggtgattc tcggtgccgc cggtaatgag
    11461 tgtcgcgccc agacccagca gtgcgcgcgc cagggcactc gc
//
LOCUS       DATMRM010000077        10205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_664931, whole genome shotgun sequence.
ACCESSION   DATMRM010000077 DATMRM010000000
VERSION     DATMRM010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664931"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /gene="lexA"
                     /locus_tag="VNZ06_02330"
     CDS             complement(<1..618)
                     /gene="lexA"
                     /locus_tag="VNZ06_02330"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HWW19618.1"
                     /translation="MSDLTPRQARVLRFIQRSLGETGMPPTRAEIAAELGFRSPNAAE
                     EHLRVLARKGVIQLLPGASRGIRLRDTMREQLALPLIGRVAAGRPILAEENVTEHLQV
                     DPAVFHPRPHYLLKVVGMSMRDAGILDGDLVAVHRTSEVRNRQIIVARLENEVTVKRY
                     RQEGSVVWLLPENPEFEPLRIDLKEESMLIEGIVVGVLRRNVAGAS"
     gene            994..1329
                     /locus_tag="VNZ06_02335"
     CDS             994..1329
                     /locus_tag="VNZ06_02335"
                     /inference="COORDINATES: protein motif:HMM:NF023267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-zipper protein"
                     /protein_id="HWW19619.1"
                     /translation="MNKTIVLKLAAAAAVVALAGCTDIKPLQADIASLKDQVSKLQTD
                     VSAAKASADAASRSASAAQSSASQAAQAASGAQSTANQALSAAQAAQSSVDATNEKID
                     RMFKKSVSK"
     gene            complement(1403..2371)
                     /locus_tag="VNZ06_02340"
     CDS             complement(1403..2371)
                     /locus_tag="VNZ06_02340"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HWW19620.1"
                     /translation="MYARIVGTGSYLPSQVVTNFDLEKRMDTSDAWIRERTGIERRHY
                     LPEGQCNIDMAEQAALKALSAAGLKPTDVDLISFGTTTPDMVFPNSGALLQARLGCRG
                     VPAFSLEAACSGFIYALAVADKFVRAGESKCALVVGSEVLSRILDHNDRSTAILFGDG
                     AGAVVLAPCEQPGIISTHLHSDGNYQHLLYCTGGPAKGPPPKGMPGGDVVHMLGSEVF
                     KLAVTLLGRVVDETLTANKLDKSAIDWLVPHQANIRIIQAVARKLDLPIERVIVTVQE
                     HGNTSTASVPLALDTGVRDGRIKTGDMLLLEALGGGVSWGSALLRY"
     gene            complement(2408..2602)
                     /gene="rpmF"
                     /locus_tag="VNZ06_02345"
     CDS             complement(2408..2602)
                     /gene="rpmF"
                     /locus_tag="VNZ06_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HWW19621.1"
                     /translation="MPVQKSRKTPSARGMHRSHDGLPAPALSVDPQSGEQHLRHRITP
                     DGFYRGRRVIEKQEEPEVPE"
     gene            complement(2634..3167)
                     /locus_tag="VNZ06_02350"
     CDS             complement(2634..3167)
                     /locus_tag="VNZ06_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HWW19622.1"
                     /translation="MPGGPSKLRDVRKLSDARAILDLDIPLAELPGLPVELIVGGGPL
                     RVQVQFGREQGFMVAEVALRGELALTCQRCMGPMPWQVDVSSPVLLIESEAQADGAPA
                     ERESYLAAEGRVSLAGLAAEELLLALPIVPLHADGAECDETGVAATAEPVAAEQGTAR
                     PFADLRALLEQGAKRKE"
     gene            3258..3836
                     /locus_tag="VNZ06_02355"
     CDS             3258..3836
                     /locus_tag="VNZ06_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HWW19623.1"
                     /translation="MASLILASSSAYRRALLARLGLPFECIAPAVDEAALPAESPHDR
                     AVRLALAKATAVASRRPGGVVIGSDQVGVCKGEPLEKPGNAERARVQLQWMSAAAATF
                     HTAVAVVQTEPAHTVQFVDTTTVYFRALAEDEIERYIAADQPFDCAGAFRCEGLGVSL
                     FTRVVSEDQTGLVGLPLISVARSLRQLGFAVP"
     gene            complement(3849..4832)
                     /locus_tag="VNZ06_02360"
     CDS             complement(3849..4832)
                     /locus_tag="VNZ06_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWW19624.1"
                     /translation="MSEPSETAPDPPPRSPVRHVEIDSEGQGQRLDKVLGRLLAGVPR
                     TRIFRLIRRGEVRVNGKRAGPEQRLNAGDSVRLPPVRAAEPHAPKRVPAALMASMERA
                     VIHEDDRVLVLDKPSGVAVHGGSGMAFGVIEALRASRPKQTLELAHRLDRDTSGVLLL
                     AKRSSALRELHALLRDGHVRKSYLALVTGRWQLGHKRIDAPLRTDLRVSGERTVKVDA
                     SGKAALTEFKLIEHYGSRASLLEAILHTGRTHQIRVHAAYCGHPVAGDEKYGDPDFNE
                     RVKAMGLKRMFLHAHSCSFAWSDNGSEQSFSAPLPAELKAVLDALNAKRRG"
     gene            5241..7718
                     /locus_tag="VNZ06_02365"
     CDS             5241..7718
                     /locus_tag="VNZ06_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HWW19625.1"
                     /translation="MKRMLVNATQQEELRVALVDGQKLYDLSIEIPSREQKKANIYKA
                     RISRVEPSLEAAFVDYGAQRHGFLPLKEISKEFFRSQPQGRMNIRELVSEGQELLVQV
                     EKEERGNKGAALTTFISLAGRFLVLMPNNPRAGGVSRRIEGEDRDQMREVMNQLQIPE
                     GMGAIVRTAGVGRSVEELQWDLDNLRTQWDAIAEAANSRGAPFLVYQESDAVTRSLRD
                     YLGDDIGEILVDDDSAYTKAQEYMQRFMPNDAARKLKRYADDIPLFTRYQIESQIESA
                     YAHKVQLPSGGSIVIDYTEALVSIDINSARATRGSDIETTACNTNLEAAEEIARQLRI
                     RDIGGLIVIDFIDMESQKNQRDVEDRLRDAMKMDRARIQIGRLSRFGLLEMSRQRLRP
                     SLGESSHLVCPRCNGIGSIRSIESLALSILRLIGEDARKDRTARLVVQLPVDVATYLM
                     NEKRDALHEIEEKSHVQMVIVPNPHIQTPEFSIRRVRDDETGLAENSQVSYQIPVAPQ
                     MSDLVDRGDKKPPAEPPAVAVMLPKTAAPVTAAPPPAAPTQPAPVPSINSSGFWGRIK
                     GWFGADELPPNEQDMPRAAPEAGSAASPTGSSPGGSHSSDRESRRDHHRRDRPERGDR
                     PERGERGDRMRGRGRRSEGRGDEPRSRDRHRDDSRRDEPRREEVRRDEPRRTEPAREA
                     AAAEGQPLADASAVRAEGDRPPRSGRSRRGGRRRRRGGSGREQGMNGQTGNGQGAEGE
                     PGHQPAHDDHDHGAAHEGPGEARHAPVETRSEPPPTPAEKPYVVWSSSPQSFSSSSGH
                     SSGPSSPPSAPAASGDSGGSEGSSHHE"
     gene            complement(7727..8464)
                     /gene="kdsB"
                     /locus_tag="VNZ06_02370"
     CDS             complement(7727..8464)
                     /gene="kdsB"
                     /locus_tag="VNZ06_02370"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HWW19626.1"
                     /translation="MFRIVVPARYASSRLPGKALLLLAGKPMIQWVVERAQRSQAQQV
                     VVATDDERIAKVVRAFAEVQMTSAAHPSATDRIAEVAVVRRWRDDEVIVNLQGDEPLM
                     PPALIDQVAHLLSAHPSTDIATLAVPLHSASELQDPNVVKVVTDLNQRALYFSRAPIP
                     FEREPSGSFAHARRHLGLYAYRVAALKRLAAAAPSALEELEKLEQLRALEHGMQIRVA
                     QVPFAPGGDVNTAADVPRVEQLLAAEK"
     gene            complement(8480..8668)
                     /locus_tag="VNZ06_02375"
     CDS             complement(8480..8668)
                     /locus_tag="VNZ06_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HWW19627.1"
                     /translation="MEARLLDILACPLCKGSLQYHRNESVLVCRGDRLAFPIRDGIPV
                     MLEDEARALTPDDPLLER"
     gene            complement(8691..9719)
                     /gene="lpxK"
                     /locus_tag="VNZ06_02380"
     CDS             complement(8691..9719)
                     /gene="lpxK"
                     /locus_tag="VNZ06_02380"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HWW19628.1"
                     /translation="MPSSNASERLQRIWYRASSPPWSLRLLSWPFALVVALRRSAYAA
                     GLLRTHRLTTPVIVVGNISVGGTGKTPLVIWLVEQLRATGMNPGVVLRGYRGSARVAD
                     AVQRVQADSDPAQVGDEALLIQQRTGAPVAVGRDRVGAARHLMSARVDLIIADDGLQH
                     LRLGRDYEIAVIDGARGMGNGYLLPAGPLREPNARLSSVGAVVVNGEGIPADLPANVG
                     PVFSMRLIGEQLHPLSGTGPSLPLASFRGRRVHALAAIGNPERFFAQLRTAGLEVIAH
                     AFPDHHAYRADELEFGDGLALLMTEKDAVKCRGFAAPDRWYLPVAASFTPEQTAALLA
                     RLQPVLEL"
     gene            complement(9712..>10205)
                     /locus_tag="VNZ06_02385"
     CDS             complement(9712..>10205)
                     /locus_tag="VNZ06_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWW19629.1"
                     /translation="ISLVSQEVVLFNDTIRNNIIFGAEDITEKKLQAAVRAAYVHEFV
                     EQLPQGLETMVGDRGVLLSGGQRQRIAIARALLRDTPILILDEATSALDTASERHIQA
                     ALDQLVKNRTTLVIAHRLSTIEHADCILVMSEGNVVECGAHAELMERGGVYAELHRLQ
                     FSA"
BASE COUNT         1905 a         3350 c         3187 g         1763 t
ORIGIN      
        1 cgacgcgccg gcgacgttcc gtcgcaatac gccgacaaca atgccctcga tgagcatcga
       61 ttcctctttg agatcgatcc gtagcggctc gaactccgga ttctctggca gcaaccaaac
      121 cacgctgccc tcttgccggt agcgcttgac ggtcacttcg ttctcgagcc gcgcgacgat
      181 gatctgccga ttgcgcactt cgctcgtgcg atgcacggcc accagatcgc catcgagaat
      241 gccggcatcc cgcatactca taccgactac tttcagaagg tagtgcggac gaggatggaa
      301 cacggcggga tccacctgca ggtgctccgt gacgttctcc tccgccagga tcggccgtcc
      361 agcggccacg cggcctatca gtggcagcgc cagctgctcg cgcatcgtgt cgcgcagccg
      421 tatcccgcgt gaggctccgg gcaacagctg aatcacgccc ttgcgcgcca gaacccgcag
      481 gtgctcctcc gcggcgttgg gtgagcgaaa cccgagttcc gcagcgatct ccgcacgggt
      541 cgggggcatt ccggtttcac ccaggctgcg ctggatgaag cgcaggacac gggcctgacg
      601 tggagtgaga tctgacatac tgtgattata tacagcttgt cccgtcggcg gcaacccgtt
      661 gcagatttcg ggcaatacgt cagtgctcac catcaatggg tcgcaggtcg acccattgcg
      721 gctacgaagt aagttcgatg gcgcacactc gatgcgtatc tatcttctta cctatgagcg
      781 cgagatgcat cggcgatcct cttgcattca tgtcgcgttc atagataccc cggtatcctt
      841 ctgacctgtc ggtaggctct gacagacgga tttcgaagta gctattgctt tcagaggtta
      901 aatttggtca agttgtggcc cgcaaaatcg ttccgccgct taaacgttcg gtgcgatgcg
      961 gttttatcca gtgcaagtgc aaaggggact agaatgaaca agacaatcgt gttgaagctg
     1021 gcggctgccg ctgcggtcgt ggcattggcc ggctgcaccg acatcaagcc gctgcaggct
     1081 gacattgcga gcctgaaaga tcaggtcagc aagctgcaga ccgacgtgtc agccgcgaag
     1141 gcttcagctg acgcagcttc tcgttcggcg agtgccgctc agagctccgc aagccaggcg
     1201 gcacaggctg ccagcggtgc gcagagcacg gccaaccagg cgctgtcggc agcacaggct
     1261 gcgcagtcgt cggtggacgc gaccaacgag aagattgatc gcatgttcaa gaagtcggtg
     1321 tcgaagtaat tcgatttctc cggtcttgag aaaaacgacg ccgcggtgac gcggcgtttt
     1381 tttgcctgcg cgattagtca ggtcagtagc gcagcagcgc cgaaccccag ctcacgccgc
     1441 ccccgagcgc ctcgagcaac aacatatcgc ctgtcttgat ccgcccgtcg cgcacgccgg
     1501 tgtcgagcgc cagaggcacc gaagcggtgg aggtattacc gtgctcctgc acggtcacga
     1561 tgacacgctc gatcggcaga tccagtttcc gggcaaccgc ctggatgatc cggatgttcg
     1621 cctgatgcgg taccagccag tcgatcgcag acttatcaag cttgttggcg gtcagggtct
     1681 catcgaccac acgtcccagc agtgtgacag cgagcttgaa cacttcgctg cctagcatat
     1741 gaaccacatc cccgcccggc atgcctttgg ggggcgggcc cttcgccgga ccgccggtgc
     1801 aatacagcag gtgctggtaa ttgccatcgg agtgcagatg cgtcgatatg atccctggct
     1861 gctcgcaagg tgccaggacc accgcccccg caccatcgcc gaacaggatc gcggtgctgc
     1921 gatcattgtg atccagaatg cgcgacagca cttccgagcc gaccaccaat gcgcacttgg
     1981 attcacccgc gcgcacaaat ttgtcggcca cggccaacgc atagatgaag ccgctgcagg
     2041 cggcctccag gctgaaggcc ggcactccgc ggcaacctag ccgcgcctgc agcagagctc
     2101 cggaatttgg gaacaccata tccggcgtcg tggtgccaaa ggagatcaga tcgacatccg
     2161 tgggcttgag gccggcggcg ctcagtgctt tgagcgcggc ctgctcggcc atgtcgatat
     2221 tgcattggcc ctcgggcaga tagtgacggc gctcgatacc ggtgcgctca cggatccagg
     2281 catcgctggt atccatgcgc ttttccagat cgaaattggt gacaacctgg cttggcaggt
     2341 agctgccggt accgacaatg cgcgcgtaca cgccctgacc cctgtactag gaccgcgggg
     2401 gccgcgacta ttccggcact tccggctctt cctgtttctc gatgacacgg cggccgcgat
     2461 agaaaccatc cggcgtgatg cgatggcgca agtgctgctc gccggactgc ggatcaaccg
     2521 acagcgccgg agcgggcaat ccatcgtgcg aacggtgcat accgcgggcc gatggcgtct
     2581 tgcggctttt ttgtacgggc atgatccaaa ctcctgcaaa ccgatccgac tgactactct
     2641 ttgcgcttgg cgccttgctc cagcaacgcg cgcaaatctg cgaacggccg tgccgttccc
     2701 tgctccgcgg cgaccggctc cgccgtcgct gccacccctg tctcgtcgca ctcggcgcca
     2761 tcggcgtgca atggcacgat gggcaatgcc agcagcaact cctccgccgc caggcctgcg
     2821 aggctcacac gtccttcagc ggcgagatag ctctcgcgct cggccggtgc accgtcggcc
     2881 tgcgcctcgg actcgatcaa tagcacaggc gagctgacgt cgacttgcca tggcatcggc
     2941 cccatgcagc gctggcaggt aagcgccagc tcgccgcgca gggccacttc ggcgaccatg
     3001 aagccctgtt cgcgcccaaa ctgcacctgt acgcgcaacg ggccgccccc cacgatcaac
     3061 tcaaccggaa gccctggcag ttcggccaac gggatatcca ggtccaggat cgcacgagcg
     3121 tccgaaagtt ttcggacgtc acgcagcttc gaggggccac ccggcatggg ccgcgtataa
     3181 taggttcagg gccccggcaa gtcaaaatta ccggtgtatc ccaacccctt gattccaaca
     3241 ccgttttcgc actttacatg gcatcgttga tccttgcttc gagttcggcg taccggcgag
     3301 cactattggc ccgactcggg ctccccttcg aatgcatcgc tccggcggtg gacgaggccg
     3361 ccctgccggc tgagagcccg cacgatcgcg cggtgcgact ggccctggcg aaggccaccg
     3421 cagtggccag ccggcgccca ggcggcgttg tcatcggcag cgatcaggtc ggtgtctgca
     3481 agggcgagcc gttggaaaaa ccgggcaatg ccgagcgcgc cagggtgcag ctgcagtgga
     3541 tgtcggccgc ggcggcgacc ttccataccg ccgtcgcagt ggtacagacc gagcctgcgc
     3601 ataccgtgca gttcgtcgat accaccacgg tgtattttcg cgcgctggcg gaggatgaga
     3661 tcgagcgcta catcgctgcc gatcaaccct tcgactgcgc cggagcgttc cgctgcgagg
     3721 gcctgggcgt cagcctgttc acgcgtgtgg tctcggagga tcagaccgga ttggtcggct
     3781 taccgctgat ctcggtggca cgctcgctgc ggcagctcgg gttcgcggtg ccctgagcaa
     3841 tcgctcgctc agcctcgccg cttggcattg agtgcatcca gcacggcctt caactccgca
     3901 gggagcggtg cgctgaagct ctgctcgctg ccgttgtccg accacgcaaa tgagcagctg
     3961 tgggcgtgca gaaacatgcg cttgagaccc atggccttca cgcgctcatt gaaatccgga
     4021 tcaccatatt tctcatcccc ggctaccgga tgaccgcaat acgcggcgtg cacgcggatc
     4081 tggtgtgtgc gcccggtatg cagaatcgcc tccagcagcg atgcacgcga tccatagtgt
     4141 tcgatcagct tgaattcagt gagggcggct ttgccactgg catcgacctt gacggtacgc
     4201 tcgccgctca cgcgcagatc ggtacgcagt ggcgcgtcga ttcttttgtg cccgagctgc
     4261 cagcggcccg tcaccagcgc caggtagctc ttccgtacgt gcccgtcgcg cagcagtgcg
     4321 tgcagctcgc gcagcgccga ggagcgcttg gccagcagca gcacgccgct ggtgtcgcga
     4381 tccagccgat gggccagttc cagagtctgc ttcggacgcg aagcgcgcag cgcttcgatg
     4441 acgccaaagg ccattccgct gccaccgtgt accgcgacgc cggagggttt gtcgagcacc
     4501 agcacccggt cgtcctcgtg gatgaccgca cgctccatcg aggccattag cgcagccggt
     4561 acgcgcttgg gcgcgtgcgg ctcggccgcg cgcaccggcg gcaggcgcac cgaatccccg
     4621 gcgttcaggc gctgctcggg accggctcgc ttaccgttga ctctcacttc gccgcgacgg
     4681 atcaggcgaa aaatgcgcgt gcgcggtacg cccgccagca gccggcctaa gaccttgtcg
     4741 aggcgctgcc cctggccctc ggaatcgatc tcgacatgtc gcaccggtga gcgcggcggc
     4801 gggtcgggtg cggtttctga tggttcgctc acgctttaag tgtttgtttc cagtaacgtc
     4861 ggtagctata atcgggccgt tgccgaagcc atagcttagg caatgagaat gcggccaagc
     4921 gccgcatggt agttggtcct cggtatcgag gccatcgcgc atgagtcggc cgatccggtc
     4981 cgcgcttcgc aagcgttagc tcctttcgaa agttagagcc cacgagcgca ccgccggtca
     5041 gtggaccttt aaaggatttc gggcgcgccg cccaagcatt ggcggccacg agtcgaaacg
     5101 aatcgaatca acatcgatga catctcgcga gcgccaacca atcttcggga tttccgctgc
     5161 cccgcgcggg tccgtcgcga tgcccccaca cgtgcatcga acggtgaccc cggcctctca
     5221 tccatttttg gcagaggcaa atgaaaagaa tgttagtcaa cgcgactcag caggaagagc
     5281 tgcgggtcgc cctggtcgat ggacagaaac tctatgacct gagcatcgag ataccttcca
     5341 gagagcagaa gaaagccaat atctacaaag cccggatctc ccgggtcgaa ccttccctcg
     5401 aagcggcatt cgtcgattac ggtgcgcagc gccacggttt cctgccgctg aaagagatct
     5461 ccaaggaatt ttttcgcagc cagccgcaag gccggatgaa tatccgcgag ctggtgagcg
     5521 aaggacagga gctattggtt caggtcgaga aggaagagcg cggcaacaag ggtgcggcgc
     5581 tgaccacctt catcagcctg gcgggccggt tcctggtcct gatgcccaac aatccgcgcg
     5641 ccggcggggt ctcgcgccgc attgaaggcg aggaccgcga ccagatgcgc gaggtcatga
     5701 accagctgca gatccccgag ggcatggggg cgatcgtccg caccgccggt gtgggtcgct
     5761 ctgtcgagga gctgcaatgg gacctggaca acctgcgcac ccagtgggac gcgatcgccg
     5821 aggccgctaa cagccgcggc gcgccgttcc tggtgtacca ggaaagcgac gccgtgaccc
     5881 gctcactgcg cgactacctg ggcgacgaca tcggcgagat cctggtggac gacgacagcg
     5941 cctacaccaa ggcgcaggaa tacatgcagc gcttcatgcc gaacgatgcg gcacgcaaac
     6001 tcaagcgcta cgctgacgat atcccgctgt tcacgcgcta tcagatcgaa agtcagatcg
     6061 aatcggccta cgcacacaaa gtccagctgc cctccggtgg cagcatcgtg atcgactaca
     6121 ccgaagcact ggtgtcgatc gacatcaact ccgctcgcgc cacgcgcggc agcgacatcg
     6181 agactaccgc ctgcaatacc aacctggaag cggccgagga gatcgcgcgg cagctgcgta
     6241 tccgcgatat cggcggtctg atcgtcatcg acttcatcga catggagtcg cagaagaacc
     6301 agcgtgacgt cgaggaccgg ctgcgcgatg ccatgaagat ggaccgcgcg cgcatccaga
     6361 tcggacgtct gtcgcgcttc ggattgctgg agatgtctcg acagcgtctg cggccgagcc
     6421 tgggcgagtc gagccacctg gtgtgcccgc gctgcaacgg catcggttcg atccgcagta
     6481 tcgaatccct ggcgctgtcg atcctgcgcc tgatcggtga ggacgcacgc aaggaccgca
     6541 ccgctcgcct cgtggtgcaa ttaccggtgg atgtggccac ctacctgatg aacgagaagc
     6601 gcgatgcgct gcacgagatc gaggagaagt ctcacgtgca gatggtgatt gtgcccaatc
     6661 cgcacattca aacgcccgag ttctcgatcc ggcgcgtgcg tgacgatgag accgggctgg
     6721 ctgagaattc tcaggtcagc tatcagatac ccgtcgcacc gcagatgagc gatctggtcg
     6781 atcgaggcga caagaaaccg ccggcggagc cgcccgcggt cgccgtgatg ctgccgaaaa
     6841 cagcggcacc ggtcaccgca gcacccccgc cggcagcgcc cacccagccg gcgccagtgc
     6901 cgagcatcaa cagttccggc ttctggggtc gcattaaggg ctggttcggt gccgatgaac
     6961 tgccgcccaa tgagcaggac atgcccaggg ccgcgcctga ggcgggctct gccgcatcac
     7021 cgaccggcag cagtcccggc ggcagccaca gctcggaccg ggagtcacgt cgagatcatc
     7081 atcgacgcga tcgcccggag cgcggcgatc gcccggagcg tggcgaacgc ggtgatcgca
     7141 tgcgcggacg cggacgacgt agcgagggcc gcggcgacga gccacgttca cgagaccgcc
     7201 atcgcgatga ctcgcggcgc gatgaacctc gccgcgagga ggtgcggcgc gacgaaccac
     7261 gccgcacgga gccagcgcgt gaggcggccg cggccgaagg ccagccgctc gccgatgcct
     7321 cagcggtacg cgccgagggt gaccggccgc cgcgcagcgg ccgttcgcgt cgcggcggtc
     7381 gacgccgacg gcgtggcggc agcggccgcg agcagggcat gaacggccag acaggcaacg
     7441 gccaaggcgc tgagggcgag cctgggcacc agcctgcgca cgatgaccac gatcacggtg
     7501 ctgctcatga aggcccgggg gaagcgcgtc acgcgccggt cgagacacgt tcggaaccgc
     7561 cgccaacgcc ggccgaaaaa ccgtacgtgg tgtggtcttc gtccccgcag tcgttctctt
     7621 cctccagcgg ccattcatcg gggccgtcga gcccgccgtc tgcgccggct gccagcggcg
     7681 attcaggcgg ttcggaaggc tcctcgcacc acgagtaagc cggacatcac ttctccgcgg
     7741 cgagcagctg ttcgacgcgg ggaacgtccg cggcggtgtt gacgtcaccg ccgggcgcga
     7801 acgggacctg cgctacgcga atctgcatcc cgtgctcgag cgcgcgcaac tgctcgagct
     7861 tctccagttc ctcgagcgca ctcggcgcag cggccgccag gcgcttgagt gcggcgaccc
     7921 ggtaagcgta cagacccagg tgcctgcggg catgcgcgaa actgccgcta ggctcgcgtt
     7981 cgaacggaat cggcgcacgg ctgaaataaa gcgctcgctg attcagatcg gttacgacct
     8041 tcacgacatt cggatcctgc agctcggagg ccgagtgcaa cggcaccgcc aacgttgcga
     8101 tatcggtcga cggatgcgca ctgagcagat gcgccacctg atcgatcagg gcgggtggca
     8161 tcagcggttc atcgccttgc agattgacga tcacttcgtc atcacgccag cgccgtacca
     8221 cggccacttc ggcgatgcgg tcggtggccg acgggtgagc ggcggaagtc atctgcacct
     8281 cggcgaatgc gcgcacgacc ttcgcgatgc gctcgtcgtc cgtggcgacc accacctgct
     8341 gcgcctgcga gcgctgcgcg cgctccacca cccactggat catgggtttg ccggcgagca
     8401 acagcagggc tttgcccggc agacggctcg aggcgtagcg tgccggaacg acgatgcgaa
     8461 acatcagcgt agatcttggc tagcgctcga gcagcggatc gtccggagtc agggcccggg
     8521 cctcatcttc cagcatcacc gggatgccgt cgcggatcgg aaaagccaag cggtcgccgc
     8581 gacagaccag caccgattcg ttgcgatggt actgcagcga tcccttgcac aacgggcagg
     8641 caagaatgtc gagtaagcga gcttccatct tgaggtctcc tgccgcgtac ctacagctcc
     8701 agcaccggct gtaaccgcgc gagcagagcg gccgtctgct cgggcgtgaa gctcgcggcg
     8761 accggtagat accagcggtc gggcgcggca aatcccctgc attttaccgc atccttctcc
     8821 gtcatcagca gcgccagtcc atcgccgaat tccagctcat cggcacggta ggcatggtga
     8881 tccgggaacg catgtgcgat gacctcgagc ccggcagtgc gaagctgggc aaaaaagcgc
     8941 tcgggattgc cgattgcggc cagtgcgtgc acgcgcctgc ccctgaagct cgccagcggc
     9001 aggctcgggc cggtcccgga cagcggatgc aattgctcgc cgatgagacg catcgaaaac
     9061 accggaccca cattggctgg caagtccgcg ggaataccct cgccgttcac caccaccgca
     9121 cccacgctcg acagccgcgc gttcggctcg cgtagcggtc cggccggtag caggtatcca
     9181 tttcccatgc cgcgcgcgcc atcgatgacc gcgatttcgt aatcgcgccc cagacgcagg
     9241 tgctgcagcc cgtcgtcagc gatgatcaga tcaacgcgcg cgctcatcag atgtcgtgca
     9301 gccccgacgc ggtcgcgtcc caccgcaacc ggagcacccg tgcgctgctg gatcagtagc
     9361 gcctcatcac ccacctgtgc cggatcactg tccgcctgga cccgttgcac agcgtccgcg
     9421 acgcgcgcgc taccgcggta accgcgcaac accacgcccg ggttcatgcc cgtggcacgc
     9481 agttgctcga ccagccagat gacgagcggg gttttgccgg tcccacccac cgagatattg
     9541 ccgaccacga tgactggggt cgtcaggcga tgtgtgcgca gcagcccggc cgcatacgcg
     9601 ctgcgtctaa gggccaccac cagggcgaat ggccacgaca acaggcgcaa cgaccacgga
     9661 ggcgaagacg ctcgatacca gatgcgctgc aggcgctcgg aggcgtttga actaggcact
     9721 gaactgcagc cggtgcagct ccgcgtagac accaccgcgc tccatcagct cggcgtgcgc
     9781 gccacactcg accacgttgc cttcgctcat caccaggatg caatccgcgt gctcgatggt
     9841 cgacagacga tgcgcgatga ccagcgtggt gcggtttttc accaattgat cgagcgcggc
     9901 ctggatgtgt cgttcggatg cggtatcgag cgctgaggtg gcctcatcca gaatcaggat
     9961 cggcgtatcg cgcagcaacg cccgtgcgat cgcaatgcgc tggcgctggc cgcccgagag
    10021 cagcacgccg cgatcgccca ccatcgtctc caggccctgc ggcagctgct cgacaaactc
    10081 gtgcacgtag gccgcgcgca ccgctgcctg caactttttc tccgtgatgt cctcggcgcc
    10141 aaaaatgatg ttgttgcgga tggtatcgtt gaacagcacc acttcctgac tgaccaggct
    10201 gatct
//
LOCUS       DATMRM010000078         7279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3755784, whole genome shotgun sequence.
ACCESSION   DATMRM010000078 DATMRM010000000
VERSION     DATMRM010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7279
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3755784"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VNZ06_02390"
     CDS             complement(<1..354)
                     /locus_tag="VNZ06_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HWW19630.1"
                     /translation="MRKLVGVALATVLSSALLIGARPSAAAIDVAISVDIAPPELPVY
                     EQPDIPGPGYIWTPGYWAWADDGSGYYWVPGTWVLAPEAGYLWTPPWWGFENGVYLWH
                     PGYWGTEIGFYGGVNY"
     gene            complement(464..1225)
                     /locus_tag="VNZ06_02395"
     CDS             complement(464..1225)
                     /locus_tag="VNZ06_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HWW19631.1"
                     /translation="MADRNLPIRSTNPTLNDRTFDGLPRALAGEAMTLQGAINKSFVL
                     LLVLMGTALYPWSQYMASGNAGDVALPLVLGLFGGLILALIISFKSTLAPYLSIPYAA
                     CEGLAIGGISAMFERRFPGIAIQAVGLTFGVMAALLFAYTSRLIRVTQTFRSVVVGAT
                     FGIMLFYLASWILSLFHVNIGLLTGVGTLSILVSLAVVVVAALNLVLSFDLIENGVAQ
                     GAPRYMEWYCAFGLLVTLVWLYMEILNLLAKVRQR"
     gene            complement(1233..2066)
                     /gene="folE2"
                     /locus_tag="VNZ06_02400"
     CDS             complement(1233..2066)
                     /gene="folE2"
                     /locus_tag="VNZ06_02400"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HWW19632.1"
                     /translation="MSTVIEPRPAKAAAVEDVQARADHRRIPIDRVGIKDIRHPVKVK
                     DRSNGEQHTIATFNMYVNLPHDFKGTHMSRFVEILHHEREISVDSFRSMLAEMRERLD
                     ADAGHIEMSFPFFVMKRAPISGVESLMDYQATLSGEICKSDTRMWIKVVVPVTSLCPC
                     SKKISEYGAHNQRSHVTIKAQLRDHIWIEELIDIAESEASCELYGILKRPDEKYVTER
                     AYDNPKFVEDMVRDVAVRLNNEPRISAYVVESENFESIHNHSAYALIEHDKEASSPSR
                     G"
     gene            complement(2093..4027)
                     /gene="dxs"
                     /locus_tag="VNZ06_02405"
     CDS             complement(2093..4027)
                     /gene="dxs"
                     /locus_tag="VNZ06_02405"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HWW19633.1"
                     /translation="MSEPAPPYPLLGAIESPADLRALPERDLPALANELRQFLIQTVS
                     TRGGHFAAGLGTVELTIALHYVFNTPDDRLVWDVGHQAYPHKVLTGRRERLFSIKQLH
                     GLAPFPSRLESPYDCFGVGHSSTSVSAALGMAIAAERQGDPRRAVAVIGDGALSAGMA
                     FEALNHAGSLPINLLVVLNDNDMSISENVGALSNYLGRVISGRLYSQLREGGKKALQQ
                     MPALRKLARRSEEHMKGMVLPGTLFEEMGFNYVGPIDGHDLKALLRALRNIRTLSGPQ
                     FLHVVTQKGKGYAPAEADPITWHGPGPFDPASGKIHKEKSATPTYSQVFGQWLCDMAE
                     RDRRIVGITPAMREGSGLVAYSKRFPDRYFDVAIAEQHAVTLGAGLACDGMKPVVAIY
                     STFLQRAYDQLVHDVALQKLPVVFALDRAGLVGSDGATHHGSYDLTFMRCLPNMVIMA
                     PSDENECRQMLFTATTINGPAAVRYPRGTGPGVAIEQDMTALPIGRAQLRRSGDSGLA
                     LLAFGVMLGPALTLAERLDATVVNMRFIKPLDSQMLCEIAASHAGLVTIEDNAVSGGA
                     GSACSEVLDANGFAIPQLHIGIPDRFIEHGSREDNLVDAGLDTPGIAATIEKWRATVP
                     ALKLTRAATKLTAAGGIGPL"
     gene            complement(4121..4366)
                     /locus_tag="VNZ06_02410"
     CDS             complement(4121..4366)
                     /locus_tag="VNZ06_02410"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HWW19634.1"
                     /translation="MTSDTPVPAEAAPFEATLAQLEELVARLESGELPLDEALRTFEQ
                     GVRMTRECQTALTAAQQKVQQLVARGDTVVVEDFQPE"
     gene            4453..4866
                     /locus_tag="VNZ06_02415"
     CDS             4453..4866
                     /locus_tag="VNZ06_02415"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HWW19635.1"
                     /translation="MKLAGTKTEKNLKDAFAGESQANRRYLYFAAKADVEGFNDVSAV
                     FRSTAEGETGHAHGHLEYLEQVGDPATGLPIGETKLNLKAAIAGETHEYSDMYPGMAK
                     QARDEGFAEIADWFETLAKAERSHANRFQKALESL"
     gene            4886..6235
                     /locus_tag="VNZ06_02420"
     CDS             4886..6235
                     /locus_tag="VNZ06_02420"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HWW19636.1"
                     /translation="MSSATGGGGREGSLEAPIRHALNWREASFYDQAALDQELHRVFD
                     ICHGCRRCFSLCQSFPTLFDAIDESPTLELDGVDKKVYWKVVEHCYLCDLCYMTKCPY
                     VPPHPWNVDFPHLMLRAKAVRAKKGELTWRQKALSATDTVGSIVGIPVIAEIANAANR
                     NRFARKLLDQALGVAPDAPVPDYHSNHARKRLKRTLRRDLSAQPTSETRGQVVLFTTC
                     YGNRNEPELAQDLAAVFEHNAIPVTLAAKERCCGMPKLELGDLQSVARYKEENVPQLL
                     KRVDAGYDIVAPIPSCVLMFKQELPLMFPEDAAVRRIGEHIYDPFEYLMLRHAAGLLK
                     TEFKTSLGKISYHVPCHLRVQNIGLKTRDVLRLVPDTTVELIERCSGHNGTYGVKSEY
                     RAASMKIGRAVISKVQSAQANYYSSDCPMAGHQIESGLQPAREPTHPLKLLRIAYGI"
     gene            6235..6819
                     /locus_tag="VNZ06_02425"
     CDS             6235..6819
                     /locus_tag="VNZ06_02425"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HWW19637.1"
                     /translation="MQKLTASDLLSLEQYARERLALRARMIDHRRLRQLRIGEHCSWS
                     FEDRDTVRYQVQEMLRTERIFEQAGIEAELGAYNPLIPDGRNLKATLLIEYEDAAQRA
                     RRLSELRGFERHCWMRVAGFDPIYAIADEDLERENDTKTSAVHFLRFEFTAQMLESLR
                     DGGGFAAGVDHPSYRHSVDPIPQRLREALMRDFS"
     gene            6883..>7279
                     /locus_tag="VNZ06_02430"
     CDS             6883..>7279
                     /locus_tag="VNZ06_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008305831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW19638.1"
                     /translation="MAESYTASDIEVLSGLDPVRRRPGMYTETTRPNHLAHEVIDNSV
                     DEAIAGYCKSIAVTLYKDQSFEVADDGRGMPVDIHPKEKVSGVELILTRLHAGGKFTE
                     GSYKFSGGLHGVGVSVVNALSKHLECWVRR"
BASE COUNT         1409 a         2252 c         2311 g         1307 t
ORIGIN      
        1 gtagttgacg ccgccgtaga agccgatctc ggtaccccag tatcccgggt gccacagata
       61 gacaccgttc tcgaagcccc accagggcgg cgtccacagg tagcctgctt cgggcgcgag
      121 cacccaggtg cccggcaccc agtaatagcc gctgccgtca tcggcccagg cccagtagcc
      181 cggcgtccag atgtacccgg ggccggggat atccggctgt tcgtacaccg gtaactcggg
      241 aggtgcgata tcgaccgaga tcgcgacatc aatcgccgca gccgaaggcc gcgcaccgat
      301 cagcagcgcg ctggatagaa cggttgcgag ggcaaccccg acaagcttgc gcataaaacg
      361 cacctctcct ggtgagcagt ctctgtcacc ttacagtgcc tcttatacgg cctagtagct
      421 gaacgacgcc tgactgagcc tgcagcctgg cgctagtcgg gcgtcagcgc tgtctgacct
      481 tggccagcag attcaggatc tccatataga gccagaccag cgtcaccagt aacccgaacg
      541 cgcagtacca ctccatatag cgaggcgcgc cctgggccac gccgttctcg atcagatcga
      601 agctgagcac cagattcagc gccgcgacca cgaccaccgc cagcgatacc agaatgctga
      661 gcgtgccgac gccggtgagc aggccgatgt tgacgtggaa caggctcagg atccacgaag
      721 ccagatagaa cagcatgatg ccgaaggtcg cacccaccac caccgagcgg aaagtctggg
      781 tgacacgaat gaggcgcgag gtgtaggcga acagcagcgc cgccatcacg ccgaacgtca
      841 ggccgactgc ctgaatggcg atgcctggaa aacgccgctc gaacatcgcc gagatgccgc
      901 cgatcgccag cccttcgcag gccgcgtatg gaatcgacag atagggcgcg agcgtcgact
      961 tgaagctgat gatcagcgcc agaatcaggc cgccgaacag acccagcacc aacggtagcg
     1021 ccacatcgcc ggcattgccg ctggccatgt actgcgacca ggggtagagc gcagtgccca
     1081 tcagcaccag cagcaacacg aacgatttgt tgatggcgcc ctgcagcgtc atcgcctcgc
     1141 cggcgagggc gcgcggcaga ccatcgaacg tacggtcgtt cagggtgggg ttggtggagc
     1201 gaatcggtag atttctgtcg gccatatatc cctcaaccgc gcgacgggga cgatgcttcc
     1261 ttgtcgtgct cgatcagcgc gtaggcagag tggttgtgaa tggattcgaa attctcgctc
     1321 tccaccacat aggcggagat gcgcggctcg ttgttgagcc gcaccgcaac gtcgcgcacc
     1381 atgtcctcga caaatttcgg attgtcgtag gcgcgctcgg tcacatactt ttcgtcgggg
     1441 cgcttcagga tgccgtagag ctcacaagag gcctcacttt cggcgatgtc aatcagttct
     1501 tcgatccaga tgtgatcgcg cagctgagcc ttgatggtca cgtgtgaacg ctggttgtgc
     1561 gcaccgtact cggagatctt cttcgagcac gggcacaggc tggtcaccgg caccacgact
     1621 ttgatccaca tgcgggtgtc gctcttgcag atctcgccac tgagcgtggc ctgatagtcc
     1681 atgaggctct ccacgccgga gatcggcgcg cgcttcatga cgaaaaacgg gaagctcatc
     1741 tcaatatgac cggcgtcggc atcgagtcgt tcccgcatct cggccagcat cgagcggaac
     1801 gagtccaccg agatttcccg ctcgtgatgc aggatctcga cgaaacgcga catgtgcgtg
     1861 cctttgaagt cgtgcggcag gttgacgtac atgttgaagg tggcgatggt gtgctgctcg
     1921 ccgttggagc ggtccttgac cttgaccgga tggcggatgt ccttgattcc gacgcgatcg
     1981 atcggaatgc gccgatgatc ggcgcgcgcc tggacgtcct ccacagcggc ggctttggcg
     2041 ggccgtggtt cgatgacggt actcataaaa aaacttctac ctgcgggtgc tcttacagcg
     2101 gaccgattcc gcccgctgcc gttaacttgg tggcggctct tgtcagcttc aaggccggga
     2161 ccgtcgcgcg ccacttttcg atcgtggccg cgatacccgg tgtgtccagc cccgcgtcca
     2221 ccaggttgtc ttcgcgactg ccatgctcga taaaacggtc cggaatgccg atgtgcagct
     2281 gcggtatcgc aaagccattg gcatccagca cttccgagca ggcgctgccc gccccgccgg
     2341 agaccgcatt gtcctcgatg gtcacgaggc cggcgtgtga ggccgcgatc tcgcacagca
     2401 tctggctgtc gagcggcttg atgaagcgca tattgacgac cgtggcatcc aggcgttcgg
     2461 ccagagtcag ggcgggcccg agcatgacgc caaacgccag cagtgccagt ccgctgtcac
     2521 cgctacggcg cagctgggcg cggccgatcg gcagcgccgt catatcctgt tcgatcgcga
     2581 ccccagggcc ggtgccgcgc ggataacgca ccgccgccgg accgttgatc gtggtggcgg
     2641 taaagagcat ctgacggcat tcgttctcgt ctgagggtgc catgatcacc atattcggca
     2701 ggcagcgcat gaacgtcaga tcgtagctgc catgatgggt ggcgccgtca ctgccaacca
     2761 gcccggcgcg atccagtgcg aacactaccg gcagcttctg cagggcgacg tcgtgaacca
     2821 gctgatcata ggcgcgctgc aggaaggtcg agtagatcgc cacgaccggt ttcatgccgt
     2881 cgcaggcgag gccggcgccc aacgtcacgg catgctgttc ggcgatggcg acgtcgaaat
     2941 agcggtccgg aaagcgcttt gagtaggcca cgagtcccga gccttcgcgc atcgcgggtg
     3001 tgatgccgac gatacgccgg tcgcgctcgg ccatgtcgca cagccattgg ccaaacacct
     3061 gcgagtacgt cggcgtggcg gacttttcct tgtggatctt gccgctggcc ggatcgaacg
     3121 gtcccggccc gtgccaggta atcggatcag cttccgccgg tgcgtaccct ttgcccttct
     3181 gggtgactac gtgcagaaac tgcggaccgg acaaggtgcg gatattgcgt agcgcgcgca
     3241 gcagcgcctt caggtcatgc ccgtcgatcg ggccgacata gttgaagccc atttcctcga
     3301 acaaggtgcc cggcagcacc atgcccttca tgtgctcttc ggagcggcgc gcgagtttgc
     3361 gcagcgccgg catctgctgc agggccttct tgcccccttc gcgcagctgt gagtacagtc
     3421 gcccggagat cacacgcccg agatagttcg acaacgcgcc gacgttctct gagatcgaca
     3481 tgtcgttgtc gttgagcacg accagcagat tgatcggcag actgccggcg tgattgaggg
     3541 cctcgaacgc catgccggcg ctgagcgcac cgtcgccgat caccgcgacg gcgcgtcgtg
     3601 gatccccctg ccgttcagcc gcgatcgcca tccccagggc ggcgctgacc gacgtactcg
     3661 agtgaccgac accaaaacag tcgtagggac tttcgaggcg tgacgggaac ggcgccaggc
     3721 cgtgcagctg cttgatcgag aacagccgct cgcgccggcc cgtcaggacc ttgtgcgggt
     3781 aggcctggtg gccgacgtcc cacaccagtc gatcgtccgg tgtattgaac acataatgca
     3841 gcgcgatcgt cagctccacc gtccccaagc cggccgcaaa gtgcccgccg cgcgtactga
     3901 ccgtttgaat caggaattgg cgcagttcat tggcaagggc cggcaggtcg cgctcgggta
     3961 gagcacgcag gtccgccggc gattcgatgg cacccagcag ggggtacggg ggggcgggtt
     4021 ctgacatgtt ggggcttaag tctacacgct ctggtcagtc ggcaccgcgc tgcagcgcag
     4081 gagcgatcgc gacgcaatgc tgcgacgcaa tagccagcgg ctactccggc tggaaatctt
     4141 ccaccacgac cgtgtcgccg cgcgcgacga gctgctgcac tttctgctgc gcggctgtga
     4201 gtgccgtctg gcactcgcga gtcatgcgta cgccctgctc gaaagttcgt aatgcctcgt
     4261 cgagcggcag ttcaccggat tccaggcggg cgacgagttc ctcgagctgt gctagcgtcg
     4321 cctcaaacgg cgccgcctcc gcaggcacag gggtatcgga tgtcatcaac tgaccctagc
     4381 atcttgcgcg aaaaaggcgc tgcattagaa taagtctaaa tttccatttg tcactgcttt
     4441 cggaggttcc agatgaaact agcgggcacc aaaaccgaga agaacctaaa ggacgcgttc
     4501 gccggcgagt cgcaggccaa ccgtcgttac ctgtacttcg cggctaaggc cgacgtcgaa
     4561 ggcttcaatg acgtctcggc agtgtttcgc tcgacggccg aaggtgagac cggacacgcg
     4621 catggacacc ttgaatacct ggagcaggtg ggtgaccccg ccacggggct gccgattggc
     4681 gaaaccaagt tgaacctgaa ggcggccatt gccggggaga cgcacgagta ctcggacatg
     4741 tacccgggca tggcgaagca ggcgcgcgat gagggctttg ctgagatcgc cgactggttc
     4801 gagacgctgg ccaaggccga gcgttcccat gcgaaccgat ttcagaaggc gctggagagt
     4861 ctgtaggggt taaggcgccg atccggtgag ttcggcgacg ggtggcgggg ggcgcgaggg
     4921 cagcctcgag gccccaatcc gccatgcgct caattggcgc gaggcgagct tctacgatca
     4981 ggccgcgctc gaccaggagc tgcatcgtgt gttcgacatc tgccacggat gtcggcgctg
     5041 cttcagcctg tgccagtcct ttcccacgct gttcgatgcg atcgatgagt cgccaacgct
     5101 ggaactcgac ggtgtagaca agaaggtcta ctggaaagtc gtcgagcact gctatctgtg
     5161 cgacctgtgc tacatgacca aatgccccta cgtgccaccg cacccgtgga acgtggactt
     5221 tccgcatctg atgttgcgtg ccaaggcggt gcgcgcaaaa aagggtgaat tgacctggcg
     5281 gcagaaagcc ctcagtgcca ccgatacggt cggcagcatc gtcggaatcc cggtcattgc
     5341 ggaaatcgcc aacgcggcca atcgcaaccg gttcgcacgc aagctgctcg accaggcact
     5401 cggcgtggca ccggatgcgc cggtgccgga ctatcattcc aatcacgcgc gcaagcgcct
     5461 gaagcgtact ttgcgccgcg acctgagcgc gcagccgacg tccgaaacgc gcggccaggt
     5521 ggtgctgttc acgacctgct acggcaatcg caacgaaccg gagctggcgc aggatctggc
     5581 ggcggtgttc gagcacaacg cgattccagt gacgctcgcg gcgaaagagc gctgctgcgg
     5641 catgccgaag ctggagctcg gggatctgca gagcgttgcc agatacaagg aagagaatgt
     5701 cccgcagctg ctgaagcggg tggatgccgg ttacgacatc gtcgcgccga ttccctcgtg
     5761 cgtgttgatg ttcaagcagg aactgcccct gatgttcccg gaggatgcag cggtccggcg
     5821 catcggtgag cacatctatg atccgttcga atacctgatg ctgcggcacg ccgccgggct
     5881 gctgaagacc gagttcaaga cctcgctcgg taagatcagc taccacgtac cgtgtcatct
     5941 gcgggtacag aacatcggtt tgaagacgcg cgacgtgctg cgcctggtgc cggataccac
     6001 ggtggagctg atcgaacgct gctccggtca caatggcacc tacggcgtga agagcgagta
     6061 tcgcgccgcg tcgatgaaga tcggccgggc agtgatctcc aaagtgcaga gcgcgcaggc
     6121 gaactattac tcgagcgatt gtccaatggc gggacatcag atcgagagcg gactgcagcc
     6181 ggcgcgtgag ccgacgcatc cgctcaagct gttacgcatc gcttacggta tctaatgcaa
     6241 aagctcacag cctccgacct gttatcgctc gagcaatacg cgcgcgaacg gctagcgctg
     6301 cgggcgcgca tgatcgatca ccgccgcctg cgacagctgc gcatcggcga gcattgctcg
     6361 tggagtttcg aggaccgcga caccgtgcgc tatcaggtgc aggagatgct gcgcaccgag
     6421 cgcatcttcg agcaggccgg gatcgaagcg gagctcgggg cctacaatcc gctcattccc
     6481 gacggccgca atctcaaggc cacgctgctg atcgaatacg aggatgcggc gcagcgcgcc
     6541 aggcggttgt cggagttgcg cgggttcgag cgccactgct ggatgcgtgt cgccgggttc
     6601 gatccgatct atgcgatcgc cgatgaggat ctggagcgcg agaacgacac caagacctcg
     6661 gcggtacatt tcctgcgttt cgaatttacc gcgcaaatgc tcgagagcct gcgcgacggc
     6721 ggcggttttg ccgccggagt cgatcatccc tcctaccggc atagcgtcga cccgattccg
     6781 cagcggctgc gcgaggccct gatgcgcgat ttcagctagc ggcgcgctcg tcggcgtgca
     6841 gaagtcagcg agctgaactg catctggtag gatgacccgc ccatggcaga gtcatacacc
     6901 gcatccgaca tcgaggtctt aagtggtctg gacccggtgc gccgtcggcc gggcatgtac
     6961 acggagacca cgcgaccgaa tcacctcgcg cacgaggtca tcgacaacag cgtcgatgag
     7021 gcgatcgccg gctactgcaa gtcgatcgcc gtcacgctct acaaggatca gtcgttcgag
     7081 gtggctgacg atggccgcgg catgccggtg gacattcatc cgaaggagaa ggtcagcggc
     7141 gtcgaactga tcctcacgcg gctgcacgcc ggaggcaaat tcaccgaagg ctcgtacaag
     7201 ttctccggcg gcctgcatgg cgtcggcgtc tcggtggtca acgcgctgtc caagcacctg
     7261 gagtgctggg tccggcgcg
//
LOCUS       DATMRM010000079         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3132133, whole genome shotgun sequence.
ACCESSION   DATMRM010000079 DATMRM010000000
VERSION     DATMRM010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3132133"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            900..1763
                     /locus_tag="VNZ06_02435"
     CDS             900..1763
                     /locus_tag="VNZ06_02435"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWW19639.1"
                     /translation="MFVAVAEHLQFTRAAEALGVTVSAASMQIQALEQYLGEPLFRRH
                     GRLVELTEQGARLLPRIREGLGALQDAIDEARSIRGRGPLRISMLSSFLVQWLMPRLP
                     AFEALHPGIELRIETSVGLVDFRASEVQAAIRLGGGSWPGLYSEKLMDEWLVAVCHPA
                     LLAKLGPVNDPPDLKRYRLLHSSTEPWSSWLLGMAPEGVIPPVISIDDSAAIVRAAEA
                     GDGLALARWSLVADDVQRGRLAIASKNVTPNGRDYYFVCPPKHCGLPKVAAFHNWLRA
                     EAARQPAPGAC"
     gene            complement(2159..>2473)
                     /locus_tag="VNZ06_02440"
     CDS             complement(2159..>2473)
                     /locus_tag="VNZ06_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19640.1"
                     /translation="HAFIVKTGQGSGESVLLKGAAARNWMEVPMPGYGGSTGNQLGAV
                     VGGRTRLPQDFAKLLYPLLVPGTTLLVTDAPMLEENTGRSMTVMGAGNPNLTMTKAAG
                     QS"
BASE COUNT          461 a          751 c          764 g          497 t
ORIGIN      
        1 cagatggaga ggcgtttcat cgatcagcgc cttcgcgcct gtgacggcgg cggggagctg
       61 cggactggca gcgagcgatg aacctagcgg agtgctgtga gcctccgtcg aggcgctgat
      121 cgacccgcgg ttagtaccag agctcactca ggaatgttag acactcgccg cgatcgactc
      181 gccgcggggt acgaggctgc ggatgatccg cagcttccag ccccgggcta cttacgtaag
      241 ttcccgccgc agcacggaat cagttcgact taagagaaac tgaattgagc tcttgcactt
      301 cgaaatccaa gtgctgcggc ggctggcact ctttgcctat ccacaacggt ggatatgaag
      361 ctcaaggagt aggctatgtt taatccgaat cgattcatgc tgggattggc tgtgctggcc
      421 ctgccgtcgg cgctgtgggc cgcagacgca gccgccgctg tgtccgccca agatcccccc
      481 tccgtgacgg tgcgatacta cgatctcaag ctgaacagtt cgcagggggt cactgatcta
      541 tatcagcgga tccgtggcgc tgccagcgag gtttgccggt cgtttgaggg ccgagagccg
      601 ctcgagcggc aggtggcacg aaatgactgc tttactcacg ccgttgcgaa cgctgtgcgc
      661 gcggtgcaca gcgacgcttt aagcgcctac cattggcagc agattcgtca gcaccgaact
      721 catgagagcg acatgccgac tagcgtggcg aggcgttgat cgatgatggc tcactcgttg
      781 gatctacgga gcgatagccg ctgtcttcga gaataaagcc gccgctgcgc cgacgactcg
      841 cgcgtcgtcg cagcggtgcg cgcgatgctt ttcgaagact gccgctgggc agccttcgtg
      901 tgtttgtcgc ggttgccgaa cacctgcagt tcacgcgcgc tgccgaagcg ctgggtgtca
      961 cggtgagcgc cgcaagcatg cagattcagg cgctcgagca gtacctcggc gagcctttat
     1021 ttcggcggca cggacgactc gttgagttga ccgagcaggg tgcgcgcttg ttacccagaa
     1081 tcagagaagg gttgggcgcg ctgcaagatg caatcgatga ggcgcggagc attcgaggcc
     1141 gcggcccttt gcgcattagc atgctcagtt cgttcctggt gcagtggctg atgccgcggc
     1201 tgccagcctt cgaggcgctg catcccggca tcgagcttcg catcgagaca tcggtcggcc
     1261 tggtcgattt tcgcgcgtcc gaagtgcagg cagcgatccg tctgggtggc gggagctggc
     1321 cgggtctcta cagcgaaaaa ctcatggacg aatggttggt ggcggtctgt catccggcac
     1381 tgctggccaa gctcggaccg gtcaacgacc cccccgatct gaagcgttat cgcttgctgc
     1441 acagctctac tgagccgtgg agcagctggc tgctgggaat ggcaccggag ggggtaatcc
     1501 cgccggtcat cagcatcgat gactctgccg ccatagtacg ggcggccgaa gccggcgacg
     1561 gcctcgcgct ggcgcgctgg agcctcgtcg ccgacgatgt gcaacgtggc aggctggcca
     1621 tcgcgagtaa gaacgtcacg ccgaatgggc gcgactacta cttcgtctgc ccacccaaac
     1681 actgtggtct gcccaaggtc gcggcatttc ataattggct gcgtgctgaa gccgcgcggc
     1741 agcccgcacc gggcgcgtgc tgaggctttg agttgtcggc accacgcacc acgccatcaa
     1801 ttgattcgat ggtggccagg ggtggaatcg acaaccgaca cgcggatttt caatccgcgt
     1861 aattctgcgc ccagcatcac ccaccgtcac ctaaaaccgc cgtttagctg atctctgcct
     1921 tacgctcggt gctcttcagt gctcataggt gacgctggat tggaaaccaa agtgggaacc
     1981 gaaaacggtg cgcccggttc cgggaacgcc aaatggtcat tcaattagca tcggcccaga
     2041 tccccgctcc gacacagtat ccgcaggatc aatattttct agatacgggc catttaaggt
     2101 cctgagtccc atgatcctga cactttgagg gcggctctca cgtgcagctc gaccgcattc
     2161 aggactgtcc agcagcctta gtcattgtta ggttcgggtt gccggcaccc attactgtca
     2221 tcgaacgacc ggtgttttcc tccagcatcg gggcatcggt aaccaacaac gtcgtccccg
     2281 gcaccagcag cgggtacagc agctttgcaa agtcctgtgg caggcgcgta cggccgccga
     2341 ccaccgctcc cagttggttg cccgtggaac caccgtaacc cggcatcggt acctccatcc
     2401 agttgcgcgc ggcggcgccc ttgagcagca cgctctcgcc gctcccctgc ccagttttaa
     2461 cgatgaatgc gtg
//
LOCUS       DATMRM010000080         4546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3756643, whole genome shotgun sequence.
ACCESSION   DATMRM010000080 DATMRM010000000
VERSION     DATMRM010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3756643"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="VNZ06_02445"
     CDS             complement(<1..1266)
                     /locus_tag="VNZ06_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19641.1"
                     /translation="MNAGAPLERSVTIYRAQQGARDSRQPQESLDLSNLLGFALVSET
                     RNVNLPAGESRIRFEGVATGIEPETALISGLPGGVIEKNQDARVLSPAQLIAETLGRD
                     IVLVRSYPKTGQSTRIPGVIRSDADGGVVFQSAEGVEALRCSGLPETFRFDARTDLSA
                     SPTLSVLVRNPKATSATLTLSYLSRGFDWTATYVAELAADSRSMDLGAWVSLANGNDV
                     SFPSTHVQVVAGQLNREKDLSDHAPLEPEVILAQCWPMGTTTSDLFSDRGALAQASAA
                     PRPQAPSGSDELFETVVTAKSARTLAALVEEEQLADLKLYRVPESTSIQSRQMKQVRL
                     LDRSDVPVEIYYAADLVADIDAAFFAARKKLRTRNDAQHHLGLPLPSGQIDTFASSHG
                     ASLLIGESAMRDTALDEEVEVELGDSSDVQ"
     gene            complement(1346..2728)
                     /locus_tag="VNZ06_02450"
     CDS             complement(1346..2728)
                     /locus_tag="VNZ06_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HWW19642.1"
                     /translation="MRRMAMGLAIAAGLVLDLASAADHDVSVTIYNDDLALVQDHRTI
                     NLTGGRQRVEFQNVSARIRPETVSLAANDLTIIEQNFDFDLLTPGKLMEKAIGQEITL
                     VRVNPATGVETREKAQVLATNGGVVLKIGERIEVLRDDGLPVRVIFDKVPDNLRAKPT
                     LSISVAGARSGSEPVTLSYLTPGLGWRADYVALFNEADSKIDVQGWVTLTNSSGTTFD
                     DAKTLLVAGSPSSSTAAGSTVYLKRPGRATLQQPGTESGTRERLGDYYLYPLSERTTL
                     ANLQTKQVSFLDVHGIPAEHGYEYRNHWLGTSAMPESAQSTYRFSTSAHAGLGDQLPA
                     GTLRFYLRDRRGEPQFIGENKIEHTPMGSTLSLATGEAFDVKVKATVDKRRQLGAYKW
                     ESDMHYELTNALPQPVTVTLLQDGLFGDTRITSESQKSTRLSDRVAQWNVSVPANGKS
                     EVTASFESRF"
     gene            complement(2783..3373)
                     /locus_tag="VNZ06_02455"
     CDS             complement(2783..3373)
                     /locus_tag="VNZ06_02455"
                     /inference="COORDINATES: protein motif:HMM:NF017747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HWW19643.1"
                     /translation="MNPDFQHLRYDDYVSMPWKNGAGTTREIAREPPLSDEFQWRLSL
                     ATIAASSPFSAYPGYRRSVTLVDGAGFRLTVSGQSPVQLSECGASALFAGSATTGCQL
                     INGTSMDLSLMVREPGVIRSVTIERCDSFRSFAAADSALQAFFCLDQGLAFAQGEAIV
                     PLALHDTILPDADAGPCDFTTTRPGSRLLRLLWQTG"
     gene            complement(3370..4182)
                     /gene="hutG"
                     /locus_tag="VNZ06_02460"
     CDS             complement(3370..4182)
                     /gene="hutG"
                     /locus_tag="VNZ06_02460"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR02017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HWW19644.1"
                     /translation="MSPEAAAIAELSLGRVPLIVSLPHVGTLLPPDVADQLTPIAASL
                     LDTDWHVDQLYDFARQSGTSWLQARVSRYAIDVNRPPDDHSLYPGQTTSGLCPTSTFA
                     GEALYPAAPPGAEEISRRRERYWVPYHAMLRELIDATRAQFGYALLLDAHSIRSQLPR
                     LFSGRLPDINLGTHDGRSCAPALSEHLMAVLQGQDRFTHVLNGRFKGGYITRTYGNPA
                     NRVHALQIELAQCAYMDESGNQYDPWTAQPLKSMLRLLVAELLKFDPSRLQT"
     gene            complement(4172..>4546)
                     /locus_tag="VNZ06_02465"
     CDS             complement(4172..>4546)
                     /locus_tag="VNZ06_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HWW19645.1"
                     /translation="QVTAAALVAENKALAHPCSVDSIPTSANQEDHVSMATHAARRVG
                     RMVDNAAAIIAVELLAAVQGLEFHRPLKSSVSLENVVTLLRTQVRRYRADRFFAPDIA
                     KAKALVTSGAFRAFVPEIGLES"
BASE COUNT          842 a         1391 c         1434 g          879 t
ORIGIN      
        1 ctgaacatca gaactgtcgc cgagttcgac ttccacctct tcgtccagcg ctgtgtcgcg
       61 catggcactt tcgccgatga gcagggaggc gccatggctg ctggcaaagg tatcgatctg
      121 cccggaaggc agcggcagcc cgagatgatg ttgcgcatcg ttcctggttc gcagcttctt
      181 gcgtgccgcg aagaaggcag cgtcaatgtc tgccaccaga tctgcggcgt agtagatctc
      241 gaccggtacg tcgcttcgat caagcagccg cacctgtttc atctgccgac tctgaatgct
      301 ggtggattca ggcacccgat agagtttgag atccgcaagt tgctcctctt ccaccagggc
      361 tgccagcgta cgggcactct ttgccgtgac gactgtttca aacaattcat ccgagcccga
      421 cggggcctga ggacgcgggg ctgccgatgc ctgcgccagc gccccccgat cagagaacaa
      481 atcgctggtg gtcgtaccca tcggccagca ttgagccagg atcacttcgg gttctaaggg
      541 cgcatgatcg ctcaagtctt tttcgcgatt gagctggccg gcgacaacct gcacgtgcgt
      601 cgacggaaag ctgacgtcgt taccgttcgc gagcgagacc caggcaccaa ggtccatgct
      661 cctggaatct gcggcgagct cggccacgta cgtggcagtc cagtcaaatc cgcgtgatag
      721 ataggacagg gtgagggttg cgctggttgc cttgggattt cgcaccagca ccgacagtgt
      781 gggtgaggca ctgagatcgg ttcttgcatc gaaacggaag gtctcgggca atccggaaca
      841 tcgcagtgcc tcgacgcctt cggccgattg aaacacgacg ccgccgtcgg catccgaacg
      901 tatcacgccc gggatgcgag tggattgacc ggtcttggga tacgaccgca ccagtacgat
      961 gtcgcgaccg agtgtttctg caatcagttg agcgggactc agcacgcgcg cgtcctggtt
     1021 cttctcgatg acacctccgg gcaaaccgga gatcagggcg gtctcgggtt ctatgccggt
     1081 tgccacgcct tcgaatcgaa ttctgctttc ccccgcaggc agattgacgt tgcgggtctc
     1141 gctcaccagc gcgaagccga gcagattgct cagatcgagc gattcctgag gttgccgcga
     1201 atcgcgtgcg ccctgctgcg cccgataaat cgtcacggag cgctcgagcg gcgctccagc
     1261 gttcacatca gcagcgtgac tgcatatcgg gaggagcccg ggaacgcata gcagcgacac
     1321 aaagcggatc cggcgcatgg ctcgatcaga agcgcgactc gaacgatgcg gtcacttccg
     1381 acttgccgtt cgccgggacg ctaacgttcc actgcgcgac ccggtcactc agtcgcgtac
     1441 tcttctggct ctccgaggtg atccgggtgt cgccgaacag gccatcctgc agcagtgtca
     1501 ctgtgaccgg ctggggcagc gcattggtaa gctcgtagtg catgtcggat tcccacttgt
     1561 aggcgccgag ctgccggcgt ttgtccacag tcgccttcac cttcacatca aaagcctctc
     1621 cggtcgcgag cgacagggtc gagcccatcg gggtgtgttc gatcttgttc tcgccgatga
     1681 actgtggctc gccgcgccga tcgcgcagat agaagcgcag tgtgccggct ggcagctgat
     1741 cgcccagacc ggcgtgcgcg ctggtcgaaa atcggtaggt ggactgcgca ctttcgggca
     1801 tcgcgctggt gcccagccag tggttgcgat attcgtagcc gtgctctgcc gggatcccgt
     1861 gcacgtcgag gaagctcacc tgtttggtct gcagattggc gagcgtagtg cgctcactaa
     1921 gcggatatag gtagtagtcg ccgagacgtt cgcgagtacc gctttcggta ccgggctgtt
     1981 gcagggtcgc gcgccccggt cgcttcaaat agacagtgct gcccgctgcg gtgctgctcg
     2041 acggtgagcc ggccaccagc aaagttttcg catcatcgaa agtggtgccg ctggaattgg
     2101 tcagggtcac ccaaccctga acatcaatct tcgagtccgc ctcattgaac agcgcgacgt
     2161 agtcggcgcg ccagcccaag ccgggtgtca ggtagctgag cgtgacgggc tccgacccgc
     2221 tgcgggcgcc ggcgacgctg atcgagagcg tcggcttggc acgcaggtta tccggcacct
     2281 tgtcgaagat gacgcgaacc ggcagaccgt cgtcgcgcag cacctcaatg cgctcaccga
     2341 tcttcagtac caccccacca ttggtcgcca gcacctgcgc tttctcgcgg gtctcgaccc
     2401 cggtggcagg gttgactcgc accagagtga tctcctggcc gatggccttc tccatcagct
     2461 ttcctggcgt cagcagatcg aaatcgaagt tctgttcgat gatcgtgagg tcattggcgg
     2521 cgagcgagac cgtctccggg cggatccttg ccgaaacatt ctggaattcc acccgctggc
     2581 gtccgccggt gagattgatg gtgcggtggt cctgcaccag cgccagatcg tcgttgtaga
     2641 tcgtcaccga tacgtcatga tcggcggccg aggcgaggtc caagacaaga cccgccgcga
     2701 tcgcaagtcc catggccatt cgacgcatga cacacctctc tatgcgcggc acgcttcaac
     2761 ctgccgcttg atttgtagtc agtcagcccg tctgccacag cagtcgcagc agccgcgatc
     2821 cgggccgtgt ggtcgtgaaa tcgcacgggc ccgcatccgc atcgggcagg atcgtatcgt
     2881 gaagcgcgag cgggacaatc gcttcgccct gcgcaaaggc cagcccctgg tccaggcaga
     2941 agaacgcctg caatgcgctg tccgctgctg caaacgaacg aaaactatcg cagcgctcga
     3001 tcgtgacgga gcgaatgacc cccggctcgc gcaccatcag gctcaagtcc atgctcgtac
     3061 cgttgatgag ctgacagccg gtcgtcgcac tgccggcaaa caatgcgctg gcaccgcatt
     3121 ccgagagttg caccggagat tgcccggaga cagtcaggcg aaagcctgcg ccatcgacca
     3181 gggtgaccga tcggcggtag ccggggtagg ccgagaacgg gctgctcgct gcaatggtgg
     3241 cgaggctcag gcgccattga aattcgtcgg acaacggcgg ttcgcgcgca atctcacgcg
     3301 tcgtgccggc gccgttcttc cacggcatcg acacatagtc gtcgtagcgc aaatgctgaa
     3361 agtcaggatt cacgtctgca gtctggaagg atcgaatttc agcagctccg cgaccagtag
     3421 tcgtagcatc gacttgagcg gttgcgcggt ccatgggtcg tactggttgc ccgattcatc
     3481 catataagcg cattgcgcca gttctatctg taacgcatgg acgcgattcg cggggttgcc
     3541 gtaagtgcgg gtgatatagc cgcccttgaa gcgaccgttg agcacatgcg taaagcgatc
     3601 ctgcccctgc agcaccgcca tcagatgctc tgagagcgcc ggtgcgcacg accggccgtc
     3661 atgggtaccg aggttgatgt ccggcaatcg tccgctgaac aggcgaggta gttggctgcg
     3721 aatggagtgg gcatccagaa gcagcgcata gccaaactgc gcgcgcgtgg catcgatgag
     3781 ttcgcgcagc atcgcgtggt acggcaccca gtagcgctcg cggcgccggc tgatctcctc
     3841 tgcgccggga ggcgccgcgg gatagagcgc ttcgcctgca aaggtggacg ttggacacag
     3901 gcccgaggtc gtctgcccgg gatagagcga gtgatcatcc ggtggccgat tgacgtcgat
     3961 cgcatagcgc gagactcgcg cctgtagcca ggacgtgccc gactgtcggg cgaagtcgta
     4021 gagctgatcc acgtgccagt ccgtgtccag gaggcttgcg gcgattggcg tcagttggtc
     4081 cgcgacgtcc ggcggcagca gcgtaccgac gtgtggcagg ctcacgatca gtggcacgcg
     4141 gcccagacta agctctgcga tggctgccgc ttcaggactc aaggccgatc tccggaacga
     4201 acgcacgaaa tgctccgctg gtcacgagcg ctttggcctt ggcaatgtcg ggtgcaaaga
     4261 atcgatcggc acggtatcgc ctgacctgcg tgcgaagcag cgtgaccacg ttctccagac
     4321 tgacggaact tttcaatgga cggtggaatt cgaggccctg cacggccgcc agcagttcga
     4381 ctgcgatgat ggccgcggca ttgtccacca tccgacccac gcgccgcgcg gcgtgcgtgg
     4441 ccatcgatac gtggtcttcc tgattcgcgg acgtcggaat gctgtccacg ctgcagggat
     4501 gggcgagggc cttgttctca gcgaccagcg ctgcggcggt gacctg
//
LOCUS       DATMRM010000081         5250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4390482, whole genome shotgun sequence.
ACCESSION   DATMRM010000081 DATMRM010000000
VERSION     DATMRM010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4390482"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VNZ06_02470"
     CDS             <1..1446
                     /locus_tag="VNZ06_02470"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HWW19646.1"
                     /translation="NANWQLSDRPDGDGLPLIRGLSMPQARATLHDARRHLSFEGSVS
                     VQNLTDRSGAPWLQILGKGQLNEAAVEFQTISDPLASASRRQPYHFSFAERSPSSQLS
                     GRGALAHAFDFDHLETRFEASGDNLHDLYALAGVSLINTGTYRLSGTMQRRDALTDFR
                     DLNATSGGSDLRGSVSVDASDERPKLSADLHSNVLRLVDLGEGAARHESVSQHPSALL
                     LSDAALKPDVVRHGEAIVRFQLQRLEATRLSLQQIAGTLSIDHGILTLSPLNAQLLDG
                     HLSGRWQLNATGDTALDTLDLHFSRLQIGQLDHPARGAPRYEALLNAQLNLAAHGSSL
                     HQIGASANGHIATQLLSGTLRESLAELSGVDLRGLGLALTRSQQQTTIRCGAAHFEAH
                     DGTLTTDDLLIDTDEVRISGQGTVQLDSEALHLELRGHPKQPRLLRLKAPVQISGTLL
                     HPHVGLEQPKSLQLIDRGSDRVVDCQALLAP"
     gene            complement(1447..2445)
                     /locus_tag="VNZ06_02475"
     CDS             complement(1447..2445)
                     /locus_tag="VNZ06_02475"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HWW19647.1"
                     /translation="MPLSNRQILIEQLPQGKLGPEHFRLHEAPRPVPADGEVLLRTRY
                     VSLDAANRAWMQGATYRSALTAGQVMAGGALAEVTESRAGNLSPGDLVFADTGWQEFV
                     TLPARQLTKIARIEPLTHLLSVYGVAGLTAYFGLLECGQPQSGETVAVSAAAGAVGEI
                     VGQIARIKGCRTVGIAGGSAKSQWLTEELRFDAAVDYKAGDTQRALRAACPDGIDVYF
                     DNVGGDVLGACLSNMKLHGRIACCGAVSQYDSASPHGPRGVPGLIVTKRLTMRGFLVS
                     DFYPKREQALRELKGWVDSGQLKVHEDVMEGLEKLPAALIGLLAGDNRGKRMVKVV"
     gene            complement(2384..3481)
                     /locus_tag="VNZ06_02480"
     CDS             complement(2384..3481)
                     /locus_tag="VNZ06_02480"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HWW19648.1"
                     /translation="MSTVLVTGGSGFIGGYCLLQLLASGHPVRTTVRNLSREGEVRAT
                     LRQAGADAGERLQFYAADLSGDAGWAEATLGCEYVLHVASPLPASAIKDENDLIVPAR
                     DGTLRVLRAARDAGVKRVVMTSSFAAIGYGHPARSAPFDETVWSNLDGAGLAPYTKSK
                     ALAERAAWDFIAREGGSLELSVVNPVMVFGPVLGSHYSTSVLILKRMLEGGLPAAPKL
                     YFGLVDVRDVADLHLRAMTHPAARGERFLAVAGNFMSVLQVAQVLRTQLGAAAARVPR
                     RQMPSWLLRLAALAVPLVRQIAPELGKIKNATNEKARRVLGWTPRSNEECIIAAAQSL
                     MRYGLLKDGIQHAAIQPPDPDRTIASGQTRT"
     gene            3642..3869
                     /locus_tag="VNZ06_02485"
     CDS             3642..3869
                     /locus_tag="VNZ06_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19649.1"
                     /translation="MNSISGSAIAGINSGLANLATDAQVVAQSVSPQQDALSLVNAIV
                     DSLDARLAVEANANVLSTANQTIGSLIDTFA"
     gene            4068..4835
                     /locus_tag="VNZ06_02490"
     CDS             4068..4835
                     /locus_tag="VNZ06_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19650.1"
                     /translation="MYRPCGLWVVLLAGLSGPALSNPTAQPAQWGDFESVRLHASQPG
                     SAAVDSYLQSSRANGDFEVDIDIADIAKPQHGTIMMVAGRVMLSKGLNLTPGSELSAL
                     DQPLLMYAVLSSTLSRVLPEGPDATVSRQKISHVDTSIGIAYASANAHGYIPPPWSVE
                     GSLRPAGNRSFEFDLLLKWSAAASRPPVVLNLKGTLQRQSDFRLDDGLEVDGFRVFSL
                     DYHSGYSAKAMTDPPRTIGDIRRQLTTPPKSPTPDKK"
     gene            complement(4842..>5250)
                     /locus_tag="VNZ06_02495"
     CDS             complement(4842..>5250)
                     /locus_tag="VNZ06_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HWW19651.1"
                     /translation="EASVRLTVRYNVIFLGIVGLIFIVLSHSLIGLFAPEPDVGRYGT
                     QALWVVSLAFPLYAAGMCLEAAFNGAGDTWTPTRLNFFCLWLGQVPIAWLLAQPLGLG
                     ALGVFIAVPVSFTILTVWSAILFRGGRWKERKV"
BASE COUNT          977 a         1787 c         1538 g          948 t
ORIGIN      
        1 aacgccaact ggcagctcag cgaccgtccg gacggcgatg gcttgccgct cattcgcggt
       61 ctatcgatgc cgcaggcgcg cgccaccctg cacgacgcgc gtcgacacct gagcttcgag
      121 ggcagcgtct cggtgcagaa tctcaccgat cgctccggcg cgccctggct gcaaatcctc
      181 gggaagggtc agctcaatga ggccgcggtc gaatttcaaa ccatcagcga cccgctcgcg
      241 agcgcgagcc ggcggcagcc ctaccatttc tcgttcgccg agcgttcgcc cagctcgcaa
      301 ctgagcggac gcggcgcgtt ggcacatgcg tttgatttcg atcatctgga gacccgcttc
      361 gaagcgagcg gtgacaacct gcacgatctt tacgcgctcg cgggtgtcag cctcatcaat
      421 accggtacct atcgcctgtc aggcacaatg cagcgacgcg acgcgctgac ggactttcga
      481 gatctgaatg cgacctccgg cggcagtgac ctgcgcggca gtgtctcggt ggacgcctcg
      541 gacgagcgtc ccaagctcag cgccgacctg cactccaatg tgctgcgcct ggtcgatttg
      601 ggcgaaggcg cggcgcgtca tgaatccgtc tcgcagcacc cctcggctct gttgctgtcc
      661 gatgccgcgc tgaagcccga tgtcgtgcgt cacggcgagg ccatcgttcg atttcagttg
      721 cagcgcctgg aggcaactcg tctgtcactg cagcagatcg ccggcacact gagcattgat
      781 cacggcattc tgacgctctc cccgttgaac gcgcagctgc tggatggcca tttgagcgga
      841 cgctggcagc tcaatgccac cggcgacact gcgctcgaca cgctggatct tcacttcagc
      901 cggctgcaga tcggacagct cgatcacccg gcccggggag ctccgcgcta cgaggcgctg
      961 ctgaacgccc agctcaacct cgccgcccac ggcagttcac tgcatcagat cggcgccagc
     1021 gccaacggcc acattgccac tcagctgctg agcgggacgc tgcgcgagtc gctggctgag
     1081 ttgagtggcg tagacctcag gggattgggg ctggcgctga cccgaagcca gcagcagacg
     1141 acgatccggt gcggcgcagc gcacttcgaa gcgcatgatg gaacgttgac gaccgatgat
     1201 ctgttgattg ataccgacga ggtacgcatc agcgggcaag gcacggtgca actggactcc
     1261 gaagcgctgc accttgaact tcgcggccat ccgaagcagc cgcggctgtt gcgcctcaag
     1321 gcaccggtgc agatcagtgg cacgctgttg cacccgcacg tcggcttgga gcaaccgaag
     1381 tcgctgcagc tgatcgatcg cggctcagac cgagtcgtag actgccaagc gctgctggcg
     1441 ccctgatcag acgactttca ccatccgctt gccgcgattg tctcctgcca gcaacccaat
     1501 cagcgccgca ggcagcttct ccagtccctc catcacatct tcgtgcactt tgagctggcc
     1561 gctatccacc cagcccttga gctctctcaa cgcctgctcg cgcttcggat agaagtccga
     1621 gaccagaaat ccgcgcatcg tcagccgttt ggtcacgatc agtcccggca cgccgcgcgg
     1681 accgtggggt gacgcactgt cgtactgcga caccgcgcca caacaagcga tgcgcccgtg
     1741 cagtttcatg ttggacaggc acgctcccag cacatcgccg ccgacgttgt cgaaatacac
     1801 atcgatgcca tccggacagg ccgctcgcag cgctcgctgc gtatcgccgg ctttgtagtc
     1861 cacggcagca tcgaatcgca gctcctcggt cagccattgg ctcttggcac taccgccggc
     1921 gatacccacg gtccgacagc ctttgatcct ggcaatctga ccgacgatct ccccgaccgc
     1981 ccctgcggcc gccgagacgg cgaccgtttc gcccgactgc ggttggccgc actcgagcag
     2041 accaaaatac gccgtcaggc cggcgacgcc gtagacactg agcaggtgtg tcagcggctc
     2101 gattcgcgcg atcttcgtga gctgtcgtgc cggcaacgtg acaaactcct gccagccggt
     2161 gtcggcgaac accagatcgc ccggcgagag atttcccgcg cggctctcgg tgacttccgc
     2221 tagcgcacca ccggccatca cctgaccggc ggtcaaggcc gaacgatagg tcgctccctg
     2281 catccaggcg cgattcgcgg catcgagcga gacatagcgc gtgcgaagca acacctcacc
     2341 atcggctggg accggccgcg gtgcttcgtg cagccgaaag tgttcaggtc cgagtttgcc
     2401 ctgaggcaat tgttcgatca ggatctggcg gttggatagc ggcatgttga attccgtcct
     2461 tcagtagacc atagcgcatt aggctctgcg cggcggcaat gatgcattcc tcgttcgatc
     2521 gcggcgtcca gcccagcacg cggcgcgctt tctcgttggt ggcattcttt attttgccca
     2581 gttccggcgc gatctgcctg accaggggca ccgcgagtgc cgccagacgc agcagccagc
     2641 tcggcatttg tcgcctcggc acgcgcgctg ccgccgctcc cagctgcgtg cgcaacacct
     2701 gtgcgacttg cagcactgac atgaaattgc cagccaccgc gaggaagcgc tcacctcgag
     2761 cggccgggtg cgtcatcgcc cgcaggtgca gatccgcaac gtcgcgcacg tccaccagcc
     2821 cgaagtagag cttcggggcc gccggcaggc caccctccag catccgtttc aggatgagca
     2881 cggaggtcga gtagtgcgaa cccagcaccg gtccgaacac catcaccgga ttgacgaccg
     2941 acagttcgag acttccgcct tcacgcgcga tgaaatccca cgccgcgcgt tccgccagcg
     3001 ccttggactt ggtatagggt gccaggcctg cgccgtcgag attgctccag accgtttcgt
     3061 cgaacggtgc cgagcgcgca gggtgtccgt aaccaatggc ggcgaacgag gaggtcatca
     3121 ccacgcgctt gaccccggca tcgcgcgctg cgcgcagcac ccgcagggtc ccgtcgcgcg
     3181 caggaacgat cagatcattc tcatctttga tcgcgcttgc tggcagcggt gacgcaacgt
     3241 gcaacacgta ctcacagccc agggtcgcct ctgcccagcc cgcgtctccg ctcagatcgg
     3301 ctgcgtagaa ctgcaggcgc tcgcccgcat ccgcaccggc ctgacgcaag gtggcccgca
     3361 cctcgccttc gcggcttaga ttgcgcaccg tggtgcgcac cgggtggcct gacgcgagca
     3421 actgcagaag gcagtagcca ccaatgaagc ccgagccgcc agtcaccaga acggtgctca
     3481 taggtcccct gtgctttcta cgggcgtcat tttgacagca cctgaatggg atgagtccac
     3541 tccggattat gtcggattcg tgacagcgcg gctgtgactg cctgcgcttc gttggacaag
     3601 ccttccccgc gccggccgac actcgtgtaa ggtctgaacc aatgaactct atctcgggta
     3661 gcgcaatcgc cggcatcaac agcggcctcg ccaatctggc gacggacgcg caggttgttg
     3721 cccaatcggt ttcaccacag caggacgccc tgtctctggt caacgctatc gtcgattccc
     3781 tcgatgcgcg tctggcggtt gaagccaacg ccaacgtgct ctcgaccgcc aatcagacga
     3841 tcggcagtct catcgacacg ttcgcttgag cgcctctgac gcacctgctg acgttgggat
     3901 ttgaccgcaa tactcaggct cttctgaggc cataacggcg ccccgaagcg ctgtggaaat
     3961 gccagcccgt agtgcctacc gccgcggtcg gtggcaacgc caactcccga tagcgttgta
     4021 ccctgtcgcg tgtcatcgtg ccgtcaacgc aatccggagc tcatctcgtg tatcggccgt
     4081 gcggtctgtg ggtggtactg ctggcaggat tgagcggccc tgccctgtct aatccgacgg
     4141 ctcagcccgc gcagtggggt gactttgagt ccgtccgttt gcatgcctca cagccaggat
     4201 ctgccgcggt ggacagctac ctgcagtcct cacgcgccaa cggcgacttt gaggtggata
     4261 tcgacatcgc cgatatcgcc aagccccagc acggaaccat catgatggtg gccggacgcg
     4321 tcatgctcag caaagggctg aacctgacgc cgggatcgga gctcagcgct ttggaccagc
     4381 cacttctcat gtacgcggtc ctgagttcga ccctgagtcg tgtgttaccc gaaggtcccg
     4441 atgccaccgt ctcgcgacag aagatctcgc acgtcgacac atcgatcggc attgcctatg
     4501 cgagtgccaa cgcgcacggc tacatcccac ccccctggtc ggtcgaaggg tcattgcgtc
     4561 ccgcaggcaa tcgctccttc gagtttgatc tgttgctcaa atggtctgcc gccgccagcc
     4621 gaccgcccgt ggtgctgaat ctcaagggaa ctcttcagcg tcagagcgac ttccgtctcg
     4681 atgacggcct ggaggtggac ggcttccgtg tcttcagtct ggactaccat tctggctata
     4741 gcgccaaggc gatgaccgac cctccgagga cgatcggcga cattcggcgg cagctcacaa
     4801 ccccaccgaa gtcgccgacg cccgacaaga agtagcgcac gctacacctt gcgctccttc
     4861 cagcgcccgc cacgaaatag gatcgcgctc cagaccgtca gtatcgtgaa ggacaccggc
     4921 accgcgataa atacaccgag tgcccccaac cctaggggtt gcgccagcag ccacgcgatc
     4981 ggaacctgcc ccagccacag acagaaaaag ttcagccgcg tcggtgtcca ggtatccccc
     5041 gctccattga atgccgcctc gagacacatc ccggccgcgt acagtggaaa ggctagactg
     5101 acgacccaca aggcctgcgt gccgtaacga ccaacgtccg gttcgggcgc aaacaggccg
     5161 atcagtgaat gcgacagcac aatgaagatc agtcccacga ttcccaggaa gatcacgttg
     5221 tagcgcacag tcagccgcac ggacgcctcc
//
LOCUS       DATMRM010000082         6368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_360139, whole genome shotgun sequence.
ACCESSION   DATMRM010000082 DATMRM010000000
VERSION     DATMRM010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6368
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360139"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VNZ06_02500"
     CDS             <1..386
                     /locus_tag="VNZ06_02500"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HWW19652.1"
                     /translation="GYGPECWGLTASDDPTGYMAHAPDNDNGTLSPTAALASFPYVPQ
                     EAMQTLRHFLSVYGDRLWRRYGFIDAFCESRDWYADTFLAIDQGPIVIMIENYRSGLL
                     WKLFMNVPEVQLGLRRLGFVSPHLT"
     gene            397..1713
                     /locus_tag="VNZ06_02505"
     CDS             397..1713
                     /locus_tag="VNZ06_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWW19653.1"
                     /translation="MSAFEQWIDQQYTHCAQAMLRSVSAVHLLKRRPGFGQSIRAAKG
                     SVIASPVLGAYDPDPDYFFHWFRDSAIVIDALRLLYQDRSIGPEALTHFADFVQFSLD
                     LQQLDGRALVSSGAWRTGVHPDFEKFVRPDAELAQVQADTVVGETRVNPDGSLDISRW
                     GRPQNDGPPLRALSVLRWLRTAPLSAPTRERAATLLRADLAYTARHWTDACYDIWEEE
                     QGLHYYTLCVSRRALDEGASWLQDSGDPAGAQALRARLPAMRTRLDQFWSPALGFYRS
                     RDLAAGAASTKELDIAVILAAIHAPSSEPAHSVHDPKMHLTLRALEQLFSADYPINHA
                     LPLGRGAAMGRYAGDVYYSGGAYYFSTLGAAEFCFRAAASGQPASSWMARGDAYLATV
                     QAYTPADGELSEQFDKRTGAQSSARQLAWSYAAFISCVGARRAVIG"
     gene            1785..3572
                     /locus_tag="VNZ06_02510"
     CDS             1785..3572
                     /locus_tag="VNZ06_02510"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HWW19654.1"
                     /translation="MTDPLEQPSTSASPELEEAAHSGLLSQLGLMRRALMSSRVGKGI
                     VLLWLLIFIVVAGTTYGQIRLNAWNKPFYDALSRRDFMDFISQLGVFLIIAGSLLIFN
                     VIQRWLAETLKYKMREGIVGDLIRDWMQPRRAFWLANAGPIGANPDQRMHEDARHLCE
                     LSSDLGVGLLQALILLAAFSGILWNLSRDFRIHIGERFYAIPGFMLWAAILYALIGSL
                     LSYWVGHTLISRNEERYAREADLRFSLVRVNEHVDGISLAAGEADETRRVELHLKAVL
                     AATARLVAGLTNLTWVTSGFGWVTLVAPTLVAAPLYFTGKLSFGGLMMAAAAFTQAQS
                     QLRWFVDNFSTIADWRAALLRVATFRRALNATEMVRHVASTITYAQGPAGVLTIDNVE
                     VVSETGCDRIRERQIEIRAGERLLIAGELGTNKTLLFRALAGLWPWGTGRVLRPTGET
                     IYYFPRGTPYLPRGTLREILAYPTKVEAFAADGFQRALERMGLQRLEPSLDETRRWDR
                     ELSPDEQLALAFARVMLQAPAWLLIDGALSALDGDTLERVFDVFTRELRSTSIINIVS
                     GSGLTRGPLFTRVVHLIKAPPGYVAGATP"
     gene            3569..5860
                     /locus_tag="VNZ06_02515"
     CDS             3569..5860
                     /locus_tag="VNZ06_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWW19655.1"
                     /translation="MIRGKFSLAVGLWVLLLMASRAWAAPQNLELHLPASASDPKMPE
                     IMKDLADRMLPVYEEPDHQRYLATLSALQLVDGNYQAAMATRQELRGLRKGADPTYPL
                     DRSVAYDIYARARNIQAHDKRAFDQAFIQSYRDLVPPLNDKDAYAIISQLEIPPAVYQ
                     NALQKLMMRYRDKKAVDLSEAVELIWAYISYEAYRGIGPLIGKLAAEDDARRYIVEPN
                     LHIKTPDGAMLSATLVRPRSVSKPLPTLLEFTIYVYPVNDAKECAAHGYVGIVAYARG
                     KRASHDPVEPFAHDGDDARSVIQWITKQSWSDGRVGMYGTAYSGYAAWAAAKEPPEAL
                     KSIVASTPTAPGIDAPKEGGIYHNAAYRWVRYVTDTKGLDTAIYNDDGHWRALDEQWY
                     RQGESYWDLMHVYHRNQADFRRWIGHPSYDGFWQKMIPYRDDFGRINFPVLTTSGYYD
                     GAEAGALYYFNEHYRYNPKADQTLLIGPYDHGVVQRGPSFELYGYQRDATALINLHEL
                     RYDWFNYVLFGAPRPAVLQDRVNFEVMGANEWQHAASLDAMGNGSLKLFLNPGQGAGT
                     RSTLASAALAAQAAGDDSFTAQSVDLADRSDAGYQPPFAIAGSTDLPAHDAIVFKSEP
                     MSKPTQISGALSGQFDLVPNKQDLDIKLALYEQLASGEYIALFDPPYAFRASYAADRV
                     HRHLLKAGERQTLGFHAERVTSRQLAAGSRLVLVLGINKRPDEQVNYGTDADVSDGTL
                     DDAKIPVKIRWYSSSFIEIPTRQ"
     gene            5872..>6368
                     /locus_tag="VNZ06_02520"
     CDS             5872..>6368
                     /locus_tag="VNZ06_02520"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HWW19656.1"
                     /translation="MPLPARLFRLTLALCLCLGPMTLAGAAADSHERVEGQEVLRATL
                     DNGLKVILVRNTLAPVAATSVNYLVGGDETPPGFPGTAHAQEHMMFRGSPDLSADQLA
                     NIGAEMGGNFNANTRESLTQYLYTVPAEDVDVALHIEALRMRDALDSEHDWSQERGAI
                     EQEVA"
BASE COUNT         1137 a         2144 c         1948 g         1139 t
ORIGIN      
        1 cgggctatgg cccggagtgc tggggactca cggccagtga cgatcccacc ggctacatgg
       61 cgcacgcgcc ggacaatgac aacggtacgc tctcgcccac cgccgcgctg gcaagctttc
      121 cctatgtgcc tcaggaagcg atgcagacgc tgcgacactt tctgagtgtc tacggcgaca
      181 ggctctggcg acgctacggc ttcatcgatg ccttctgtga atcgcgcgac tggtacgccg
      241 atacgtttct ggccatcgac cagggaccga tcgtgatcat gatcgaaaat taccgcagcg
      301 gtctgctgtg gaagctcttc atgaacgtgc cggaagtgca gctcggactg cgtcgcctcg
      361 gcttcgtcag cccccacctg acttagcgcc tgacgcatga gcgcgttcga acaatggatc
      421 gatcaacagt acactcactg cgcgcaggcc atgttgcgca gcgtctccgc ggtccatctg
      481 ctcaagcgcc ggcccggctt cggccagagc atccgcgcgg cgaaaggttc ggtcattgcc
      541 tcccctgtgc tcggcgcgta cgacccggat ccggattatt tctttcactg gttccgtgac
      601 tccgcgatcg tgatcgatgc cctgcggctg ttgtaccagg accgcagcat cgggccggaa
      661 gccctcacgc atttcgccga tttcgtgcag ttcagtctgg atctgcagca gctcgacggc
      721 cgcgcgctgg tctcctccgg cgcctggcgg accggcgtgc accccgactt cgaaaaattc
      781 gtccgccccg atgccgagct ggcgcaggtg caagctgaca ccgtggttgg cgagacccgt
      841 gtcaaccccg atggttcgct cgacatatcc aggtggggcc ggccccagaa cgacggacca
      901 ccgctgcggg cgctgagcgt gttgcgctgg ctgcgcacgg cacccctgag cgcaccgacc
      961 cgagagcgtg ccgcaacctt gctgcgtgcc gacctggcgt acaccgcccg tcactggacc
     1021 gatgcctgct acgacatctg ggaagaggag caaggtctgc actactacac gctgtgtgtg
     1081 tcgcgacgcg cgctcgatga aggcgctagc tggctgcagg acagcggcga ccccgccggc
     1141 gcgcaggcgc tgcgcgcgcg ccttcctgcg atgcgcaccc ggctcgatca gttctggtcg
     1201 cccgcgctgg gcttctatcg ttcgcgcgat ctggccgcag gggcagcctc caccaaggaa
     1261 ctcgacatcg cggtcattct ggccgcgatt catgctccca gcagcgagcc cgcacattcc
     1321 gtgcatgacc cgaaaatgca cctgaccctg cgcgcgctcg agcaactctt cagcgccgac
     1381 tatccgatca atcatgcgct gccgctcggg cggggagctg cgatgggacg ctatgccggt
     1441 gatgtctatt actccggcgg cgcctattat ttttcgacac tgggagcggc cgagttttgc
     1501 tttcgtgcgg ccgccagtgg gcagccggcg agcagctgga tggcgcgcgg ggatgcatac
     1561 ctggcgaccg tgcaagccta tacacctgcc gatggtgagc tgtcggagca gttcgacaag
     1621 agaacggggg cgcaaagctc ggcccggcaa ctcgcgtgga gctatgctgc gttcatctct
     1681 tgtgtcgggg cgcgccgtgc tgtcataggt taacttacat taactatgac ttgtaataca
     1741 ttgtaataac agtagatatg ctcgcctcct tagactaacg cacgatgacg gatcccttag
     1801 aacaacccag caccagcgcc tcgcctgagc ttgaggaggc ggcgcattcg gggctgctct
     1861 cacagcttgg gctgatgcgc cgggcactga tgtcctcgcg cgtcggcaag ggcatcgtgc
     1921 tgctctggtt gctgatcttc attgtcgttg ccggcaccac ctacggacag atccggctca
     1981 acgcctggaa taaaccgttc tacgatgccc tctcgcgacg cgacttcatg gacttcattt
     2041 cccagctcgg cgtgttcctc atcatcgcgg gcagcctgct gatcttcaac gtgatccagc
     2101 gctggctcgc cgaaaccctc aaatacaaga tgcgcgaggg tatcgtcggc gatctcattc
     2161 gcgactggat gcagccgcga cgcgcgttct ggctcgccaa cgccggcccg atcggtgcca
     2221 atcccgatca gcgcatgcat gaggatgcgc gccacctgtg cgaactgtcg tccgacctgg
     2281 gcgtgggatt gctgcaggcg ctgatcctgc tggccgcctt ttccggcatc ctgtggaatc
     2341 tgtcacgcga cttcaggatc cacatcggcg agcgcttcta cgcgatcccg ggattcatgc
     2401 tgtgggccgc gatcctgtac gcgctgatcg gctcgctctt gagctactgg gtcggacaca
     2461 cgctcatctc gcgcaatgaa gagcgctatg cgcgcgaagc ggacctgcgc ttctcgctgg
     2521 tacgcgtcaa cgaacatgtc gacggcatct ccctggcagc gggcgaagcg gatgaaaccc
     2581 gccgcgtcga actgcatctg aaggccgtgc tggcagccac cgcccgcctg gtggcgggac
     2641 tgaccaatct cacctgggtc acctccggat tcggctgggt cacactggtg gccccgacgc
     2701 tggtcgccgc cccgctctac ttcaccggca agctctcctt cggcggcctg atgatggctg
     2761 cggccgcttt cacgcaggcc cagtcgcagt tgcgctggtt cgtagataat ttcagcacca
     2821 tcgccgactg gcgcgcggcg ctgctgcgcg tcgccacgtt ccgccgtgca ctcaacgcca
     2881 ccgagatggt gcgccacgtc gccagcacca tcacctacgc gcaagggcca gcgggcgtac
     2941 tgacgatcga caacgtcgag gtggtctccg aaaccggctg cgaccgcatc cgcgagcgcc
     3001 agatcgaaat ccgcgctggc gagcggctgc tgattgccgg tgagctcggc accaacaaga
     3061 cgctgctgtt ccgcgcgctg gcggggctgt ggccgtgggg tacgggacgg gtgctgcgtc
     3121 ccaccggaga gacgatttac tacttcccgc gcgggacgcc ttatctgccg cgcggcacgc
     3181 tgcgcgagat cctggcatac ccgacgaaag tggaggcgtt tgcggccgat ggattccagc
     3241 gggcgctcga acgcatggga ttgcagcggc tggagccgtc gctggacgag acgcgccgct
     3301 gggaccggga actcagtccc gatgagcagc tggccctcgc ctttgcgcga gtgatgctgc
     3361 aggcccctgc ctggctgctg atcgatggcg cgctcagcgc gctcgacggc gacacgctgg
     3421 agcgtgtatt cgacgtgttc acccgcgagc ttcggtccac cagcatcatc aatatcgtca
     3481 gcggtagcgg cctgacacgc gggccgctgt tcacacgggt ggttcatctg atcaaggcgc
     3541 cgccgggcta cgtcgccgga gcgaccccat gattcgcggg aaattcagcc tcgcggtagg
     3601 gctgtgggtg ctgctgctca tggcgtcgcg cgcctgggcc gcgccacaga acctcgagct
     3661 gcacctgccg gcctctgcct ccgatccgaa gatgcccgag atcatgaagg atctcgctga
     3721 tcgcatgctg ccggtgtacg aagagcccga tcatcagcgc tatctggcga cgctctcggc
     3781 gctgcagctg gtggacggca actatcaggc ggcgatggcc acacgccagg agctgcgcgg
     3841 gctgcgcaaa ggcgccgatc ccacctaccc gctcgatcgc tcggtcgcct atgacatcta
     3901 cgcgcgcgcc cgcaacatcc aggcgcacga caagcgagca ttcgatcagg ccttcattca
     3961 gtcgtatcgc gatctggtgc cgccgctgaa cgacaaggac gcctacgcga tcatctcgca
     4021 gctcgagatc ccgcccgccg tctatcagaa cgcgctgcag aaactgatga tgcgctaccg
     4081 ggacaaaaaa gccgtggatc tgtccgaggc cgtggagctg atctgggcct acatctccta
     4141 cgaggcctat cgcggcatcg gcccgctgat cggcaagctt gccgccgagg acgacgcgcg
     4201 acgctacatc gtcgagccca acctgcacat caagacgccg gacggcgcca tgctctcggc
     4261 taccctggtg cgcccgcgct cggtgagcaa accgctgccg acgctgctgg aattcacgat
     4321 ctacgtctac ccggtgaatg atgccaagga atgcgccgcg cacggctacg tcggcatcgt
     4381 cgcctacgcg cgcggcaagc gtgccagcca cgacccggtc gagccgttcg cgcacgatgg
     4441 cgatgacgcc cgatcggtca ttcagtggat cacgaagcag tcctggagtg acggtcgcgt
     4501 cggcatgtac ggcacggcgt acagcggcta tgccgcctgg gccgccgcga aggagccacc
     4561 cgaggcgctg aagagcatcg tcgcctccac gccgaccgca ccgggtatcg acgcaccgaa
     4621 ggaaggcggc atctaccaca acgccgccta tcgctgggta cggtacgtca ccgacaccaa
     4681 aggcctggac accgccatct acaatgacga tgggcattgg cgcgcgctcg atgagcagtg
     4741 gtaccgtcaa ggcgaatcct actgggacct gatgcacgtc tatcaccgca atcaggcgga
     4801 ttttcgtcgc tggatcggcc acccgagcta cgatggcttc tggcagaaaa tgatccccta
     4861 tcgggatgat ttcggccgca tcaacttccc cgtcctgacc acttccggtt actacgatgg
     4921 cgctgaggcc ggcgcgctct actatttcaa cgagcactac cgctacaacc ccaaggccga
     4981 ccagaccctg ctgatcggtc cctacgatca cggcgtggtt cagcgcggtc cgtcgttcga
     5041 gctctatggc tatcagcgcg atgccacggc gctcatcaac ctgcatgagc tgcgctacga
     5101 ctggttcaac tacgttctgt tcggtgcccc cagacctgca gtgctgcagg atcgcgtgaa
     5161 ctttgaggtc atgggcgcca atgaatggca gcatgccgcg tcgctcgacg cgatgggcaa
     5221 tggctcgctc aaactgttcc tcaacccggg ccagggcgcg ggcacgcgca gcacactcgc
     5281 cagcgcagcg ctggccgcac aggcggccgg tgatgacagc ttcaccgccc agagcgtcga
     5341 tctggccgat cgcagcgatg ccggctacca gccgccgttt gcgatcgccg gcagcaccga
     5401 cctgcccgcg cacgacgcca tcgtgttcaa gagcgagccg atgtcgaagc cgacgcagat
     5461 cagcggcgcg ttgtccgggc agttcgactt ggtgcccaac aaacaggacc tcgacatcaa
     5521 actggccctc tacgagcagc tcgcgagcgg tgaatacatc gcgctgtttg atccgcccta
     5581 cgcgtttcgc gccagttacg ccgccgaccg cgtacaccgc cacctgctga aggccggcga
     5641 gcggcagacg cttggctttc acgccgagcg cgtcaccagc cgccaactgg ccgctggcag
     5701 ccgcctggtg ctggtgctcg gcatcaacaa acgccccgat gagcaagtca actacggcac
     5761 ggacgcagat gtcagcgatg gaacgctcga tgacgctaag attccagtga agattcgatg
     5821 gtacagctca agcttcatcg aaatccccac ccgacaataa ggtccctcat catgccattg
     5881 cccgcgcgcc tcttccgcct caccctagcg ctgtgcctgt gtctcggacc gatgacgctc
     5941 gccggcgccg cggccgactc gcatgagcgc gtcgaaggac aggaggtgtt gcgagccacg
     6001 ctcgacaacg gcctgaaggt catcctggtg cgcaatacgc tcgcccccgt ggccgccacc
     6061 tcggtcaact atctggtcgg cggcgacgag acccctcccg gcttcccggg caccgcccac
     6121 gcccaggagc acatgatgtt ccgcggcagc cccgacctct ccgccgatca gctcgccaac
     6181 atcggagcgg agatgggagg caacttcaat gccaatacgc gcgagagcct cacgcagtac
     6241 ctgtataccg tgccggcgga agacgtggac gtcgcactgc atatcgaagc actgcgcatg
     6301 cgggacgcgc tcgacagcga gcacgactgg tcgcaggagc gcggcgcgat cgaacaggag
     6361 gtcgcgca
//
LOCUS       DATMRM010000083         3203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2203624, whole genome shotgun sequence.
ACCESSION   DATMRM010000083 DATMRM010000000
VERSION     DATMRM010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2203624"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..3171)
                     /locus_tag="VNZ06_02525"
     CDS             complement(10..3171)
                     /locus_tag="VNZ06_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW19657.1"
                     /translation="MLAKFFIERPVLANVIAILTMVLGAVAIFALPVAQYPPITPPTV
                     QVTTRYPGANATTLINTVALPIEQQVNGVEDMLYMQSTSTNDGNYTLTVTFKVGTDLD
                     FAQVLVQNRVAAAIASLPAAVQTNGVVTRKKSTAILEIVTLTSADARYDSLFLNNYAS
                     IRMRDVLARLPGVGDVTVFGIGQYSMRVWLDPQKLLARSLTPNDVINVIRQQNQDVAA
                     GQIGTPPAASGQDFQLTVNVPGALNDVAEFENIIVKTDAAAQGSRAITRLKDVARVEL
                     GAQTYSQFFTMDGKQAAGIAIFQLPEANALDVATGVRAKLATLAKDFPQGITYSIPFD
                     TTKFVSASIHEVYQTLIEAGILVLIVILVFLQNWRATLVPATTVPVTIIGAFAAMAAM
                     GFSINILTLLSVVLAIGIVVDDAIVVVEGVSHNIERGMAPKQASIEAMRELFAPIIGI
                     TLVLMAVFLPAAFMPGITGQMYRQFALVIAATALISAINAATLKPTQCALWLRPIPPG
                     QTKNWFYRGFDAVYNPVERAYVRLIARMVERSGRTFAIAAVLIVASALALTRVPTGFI
                     PLEDQGYLLIAAQLPDAASLERTQRVLEQISAIALKTPGVEHAITIGGISALDGNASL
                     ANAGVIYLTLADWDVRYKHKGQDLKSLYQKLARDLAQLEAARVQVVPPPPIQGIGMSG
                     GFQMQVELTDGTFDYRKLQRISDLLVSESNTQSGLERVMTTFRADVPQISAEINRSTA
                     QSLNVPIGSIFDTLQSYLGSTYANQIQKFGRVFPVFVQADSEFRLTPQDLRRYYVRSS
                     SGDMVPIGTLADVEYTQGPALISMYNLYPSSSIIGMTATGFSSGQSLQTMEQLASRTL
                     PPGTSSEWTAMSYQEKLAGRSAYLIFGLALLLVYLVLAGQYGSWVTPAAVILGVPLAL
                     LGTAGALLAIGIANNLYTQIGLVLLIALSAKNAILIVEVARELRDAGQSIHDAAVEAA
                     RKRFRPIVMTSFAFILGVVPLVLAKGAGAAARASIGIAVFSGMLASTCLAVLFVPSFF
                     VILQRWQERHRKPTVAEARADAR"
BASE COUNT          550 a          968 c         1142 g          543 t
ORIGIN      
        1 gcgccggcct tagcgggcgt ccgcgcgggc ttcagcaacc gtgggctttc gatgacgctc
       61 ctgccagcgt tgcaggatga cgaagaaaga gggtacgaac aggaccgcca ggcaggtcga
      121 ggcgagcatg ccgctgaaca ccgcgatacc gatggaggca cgtgcggcgg ccccggctcc
      181 cttggccagt accagcggca ctacgccgag gatgaaggca aaggaggtca tgacgatcgg
      241 ccggaagcgc ttgcgcgccg cctcgacggc ggcgtcgtgg atgctctgac cggcgtcgcg
      301 cagctcacgc gccacctcca cgatcagaat cgcgttcttc gccgacaggg cgattaggag
      361 cacgagcccg atctgcgtgt agagattgtt ggcgatgccg atcgccagca gtgcaccggc
      421 ggtgccgagc agcgccagcg gcacgccgag gatcacggcc gccggggtca cccagctgcc
      481 gtactggccg gcgagcacca ggtataccag cagcagcgcg agaccgaaga tgaggtacgc
      541 ggaacggccg gccagctttt cctgatacga catggcggtc cactcgctgc tggtgcccgg
      601 cggcagcgtg cgcgacgcca gctgctccat ggtctggagc gactggccgg aactgaaccc
      661 ggttgcggtc atgccaatga tgctgctgga ggggtagagg ttgtacatgc tgatgagcgc
      721 cggtccctgg gtgtactcca cgtccgcgag tgtgccgatc ggcaccatgt cgccgctgga
      781 gctgcgcacg tagtagcggc gcaggtcctg gggcgtgaga cggaactcgc tgtccgcctg
      841 cacgaacacc gggaacacac gcccgaactt ctggatctgg ttggcgtagg tcgaacccag
      901 ataactctgc agcgtatcga agatgctgcc gatggggacg ttgagcgact gcgccgtgga
      961 gcggttgatc tcggcgctga tctgcggcac gtcggcgcga aaggtcgtca tgacgcgctc
     1021 gagtccgctt tgcgtattgc tctcgctcac cagcagatcc gagatgcgct gcagcttgcg
     1081 gtagtcgaac gtgccgtcgg tgagctcgac ctgcatctga aagccccccg acatgccaat
     1141 gccctggatg ggcgggggcg gcaccacctg cacgcgcgcg gcttccagct gggcgaggtc
     1201 gcgcgcgagc ttctgataca gggacttgag gtcctgcccc ttgtgcttgt agcgcacgtc
     1261 ccagtcggcg agggtgagat agatgacgcc ggcgttggcg agcgaggcgt tgccatcgag
     1321 cgcggagatg ccgccgatgg tgatggcgtg ctccacgccc ggggtcttga gcgcgatggc
     1381 gctgatctgc tcgagcaccc gctgggtgcg ctcgagcgag gcggcgtccg gtaactgggc
     1441 ggcgatcagc aggtatccct ggtcctcgag cggaatgaag ccggtcggca cgcgcgtgag
     1501 ggccagggcg gaggccacga tcagcacggc cgcaatggcg aaggttctac cgctgcgctc
     1561 gaccatgcgc gcgatgaggc gcacataggc gcgctcgacc gggttgtaca cggcatcaaa
     1621 gccgcggtag aaccagttct tggtctgccc cggcgggatc ggtcgcagcc acaacgcaca
     1681 ctgcgtcggc ttgagggtgg cggcgttgat cgcgctgatg agcgcggtgg cggcaatgac
     1741 gagggcgaac tgacggtaca tctggccggt gatgccgggc atgaaggcgg ccggcaagaa
     1801 caccgccata agcaccagag tgatgccgat gatgggggcg aacagctcgc gcatcgcctc
     1861 gatggaggct tgcttcggag ccatgccgcg ttcgatgtta tggctcaccc cctcgaccac
     1921 cacgatggcg tcgtcgacca cgatgccgat cgccagcacc accgacagca gcgttaggat
     1981 gttgatcgag aatcccattg ccgccatggc cgcgaacgcg ccgatgatcg tgaccggcac
     2041 cgtggtcgcc ggcaccaggg tcgcgcgcca gttctgcagg aacacgagga tgacgatcag
     2101 caccaggatg cccgcctcga tcagggtctg gtagacctcg tggatcgagg cggagacgaa
     2161 cttggtggta tcgaagggga tgctgtaggt gatgccctgg gggaaatcct tcgcgagggt
     2221 ggcgagcttg gcacgcacgc cggtggcgac gtcgagggcg ttggcctcgg ggagctggaa
     2281 gatcgcgatg ccggcggcct gcttgccgtc catggtgaag aactggctgt aggtctgggc
     2341 gccgagctcg acccgcgcga catccttcag gcgcgtgatg gcgcggctgc cctgggcggc
     2401 tgcatcggtc ttgacgatga tgttctcaaa ctccgccacg tcgttgagcg cccccgggac
     2461 attcacggtc agctggaagt cctggccact ggccgccggc ggcgtgccga tctggccggc
     2521 ggcgacatcc tggttctgct ggcggatcac gttgatgacg tcgttgggcg tgagcgagcg
     2581 tgccaggagc ttctgcggat caagccacac ccgcatgctg tactggccga tgccgaacac
     2641 cgtgacgtcg ccaactcccg gcagacgcgc gagcacgtcg cgcatgcgga tcgaggcgta
     2701 gttgttgagg aacaggctgt cgtagcgcgc atcggcactg gtgagcgtca cgatctcgag
     2761 gatggccgtg gacttcttac gggtgaccac gccattggtc tgtaccgccg cggggagcga
     2821 ggcgatcgcc gcggccacgc ggttttgcac cagcacctgg gcaaaatcca gatccgtacc
     2881 gaccttgaag gtcacagtga gcgtgtagtt gccgtcgttg gtgctcgtcg actgcatgta
     2941 gagcatgtct tcgacgccgt tgacctgttg ctcgatcggc agcgccacgg tgttgatcag
     3001 ggtcgtggcg ttcgcgccgg ggtagcgggt ggtgacctgc accgtggggg gggtgatcgg
     3061 cgggtactgc gccaccggca gcgcgaagat cgccaccgcg ccgagcacca tcgtcaggat
     3121 cgcgatgacg ttggcaagga ccggccgctc gatgaagaac ttagccagca tgctagctct
     3181 ggctacgggc gtgcgctggg ggc
//
LOCUS       DATMRM010000084         6060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1601144, whole genome shotgun sequence.
ACCESSION   DATMRM010000084 DATMRM010000000
VERSION     DATMRM010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1601144"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VNZ06_02530"
     CDS             <1..446
                     /locus_tag="VNZ06_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19658.1"
                     /translation="ELQRVMDQAKATDPGIQYANVLDANLYAPSFHADGAQDWTGIPE
                     KDMLGNRMKRIYDGRWSSPDSVRVGMGPNAKKVPDRASRQQFIDAGCELAEHPAIVEQ
                     FMVRVYVRDSSDVVLTLSVPIFVRGQRYGAVSLGWTANPNQSLSA"
     gene            complement(965..1948)
                     /locus_tag="VNZ06_02535"
     CDS             complement(965..1948)
                     /locus_tag="VNZ06_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HWW19659.1"
                     /translation="MHAMLLTRTGHALDWCELEDAIPGPNEILVRVSACGVCRTDLHV
                     VDGDLPDSKLPIIPGHEIVGRIEVLGAGVDHLDTGQRVGVSWLGHTCGRCSYCHSDRE
                     NLCEHPLFTGHTRDGGFADKVIADARYVFPLGEAGSDVSLAPLLCAGLIGWRALKMAG
                     SGARLGLYGFGAAAHIIAQVARHQERQVFAFTRANDLRSQAFARQLGAVWAGGSEQAP
                     PEPLDAAILFAPVGELVPLALRAVRKGGRVVCAGIHMSDLPSFAYRLLWEERQLLSVA
                     NLTRQDGFEFLRHASDFHIETQTTTYPLLQANQALADLRAGAFDGAAVLVP"
     gene            complement(1963..2961)
                     /locus_tag="VNZ06_02540"
     CDS             complement(1963..2961)
                     /locus_tag="VNZ06_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWW19660.1"
                     /translation="MNCIRRILVSVKDPAQGVTPAASKAAQLARATGATLELFHAITT
                     PVYLDNYTYSDWTLTDAKRDVMKRRTEQMQRIAHDLEVRPGQKCVRTLITAAWDWPAY
                     AAIVRQAAASRADLVLVDRHAGRHLARTLMRYADWELLRLCPAPLLLVGKDSLYTRPL
                     TLAAVDPAHYWDKSARLDERILDAATTVSTALRGTLHALHTFESTLPVSYPTDVMYPK
                     LSRDLAPRLRTAASKRFSRLLRGRRIGQARQHLIDAPPALGIAQAARKLHADLLVMGA
                     VSRSGLKGLLIGNTAEILLDRVTCDLLIIKPPRFKTRVARIQTGARIVPALPPVMA"
     gene            complement(3200..3859)
                     /locus_tag="VNZ06_02545"
     CDS             complement(3200..3859)
                     /locus_tag="VNZ06_02545"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWW19661.1"
                     /translation="MSTDLRLRQDVETQLDWDPRFDSQKIGVSVKDGVVALTGQVSSY
                     ADWHAAQDAAQSVAGVHVLANELRIMDHPDQSRSDADIAEAAMHVLRNNIHVPLQHLK
                     LIVSGAWITLTGHVPNWFQRDAIETALRPVRGVIGISNKVDIQPIVKVDDIRDRIQAA
                     FHRHASIDASNIRISVTDGEVVLEGTVRSLPERGDAEFAAWNIPGVRHVTDQLTVKPS
                     L"
     gene            complement(3955..4734)
                     /locus_tag="VNZ06_02550"
     CDS             complement(3955..4734)
                     /locus_tag="VNZ06_02550"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HWW19662.1"
                     /translation="MHEQYRLLRAQLAPLRRRSSPARVHDSRIAARQLRSTLSALAPA
                     LPGRARRALRHELKTLSDHLGAARDARVRTDLLLPLLQRHPVTDRRQQTLVLLRAASR
                     QASARAHRWLNAAAFDQCLRRMDRAAKTLTRAAQPSPNAKALLREALLDRWSRLRHAP
                     KNTPLAPDDIHRLRLMTKKCRYLLAIYPPTHHASTREWLKQLQGCLGDLHDLQQARGW
                     LQQQHGLRPPDRVLRIRLGARTARLTTQLRALLRTQPSLLL"
     gene            complement(4921..5544)
                     /locus_tag="VNZ06_02555"
     CDS             complement(4921..5544)
                     /locus_tag="VNZ06_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW19663.1"
                     /translation="MNPTLASAQPTVFVVDDDDAVRSSLRMLLKSVGRTVVDYASATA
                     FLAAYKFDRPGCLVLDIRMPGMSGLELQDQLNERGAIVPVIFISGHADVPMAVEAVQH
                     GAFDFLQKPFRDQDLLDRVQAAIERDSANRRQLRERESLQKRLNSLTDREREVLDLVT
                     QGKANKVMAGDLGVSQRTVEIHRARVMEKMEASSLAQLVRMMIVLEG"
     gene            complement(5563..>6060)
                     /locus_tag="VNZ06_02560"
     CDS             complement(5563..>6060)
                     /locus_tag="VNZ06_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW19664.1"
                     /translation="TISHLRKLVGTPAGRREPTDINELIREIGNLTRADARDHNVQVQ
                     WQLLSDLPPLNVDRIQIQQVLLNLFRNAVDALATLPAGPRELYIRSALDPQGDVTVSV
                     ADNGPGVAADMVPKLFTPFSTHKIHGTGLGLAISRSIVEAHRGRLEYQPNAPRGAVFA
                     MTLPH"
BASE COUNT         1155 a         1827 c         1935 g         1143 t
ORIGIN      
        1 tggaactgca acgggtgatg gaccaggcca aggccaccga cccggggatt cagtacgcca
       61 acgtgctgga cgctaatctg tatgcaccgt cctttcacgc cgacggtgca caggactgga
      121 ccggcatccc cgagaaggac atgctcggta atcgaatgaa gcgcatctac gacggcagat
      181 ggtccagtcc ggattccgtg cgcgttggca tggggccgaa cgccaagaag gtccccgacc
      241 gtgcctcacg tcagcagttc attgacgccg ggtgcgagct tgccgaacat ccagctatcg
      301 tcgaacaatt catggtcaga gtctacgtca gagactcgag cgatgttgtc ctgaccctgt
      361 cggtgccgat ctttgtcagg ggacagcgtt acggcgcggt atcgctcggt tggaccgcga
      421 atccaaacca atccctgtct gcctgattgc ccgatccgac gggatagatt ctaagccttc
      481 gcgctagtca gaatgggggg acgtcggacc aggcggcatc ctggaataag tactatcccg
      541 cattgtcgcg aggccctcgg tccgcatagc ctgcttggca ctcttccaat tcggagcaaa
      601 agcaattttg gccgaaaaga caaagagcga tgaggagtcg tttcgatcgt acccgagtga
      661 gttcggactg gagcagatat gttcactatg tttcatcacg tgaccgtgtg gtcagcggtg
      721 gccttggtcg ccgctctcag tctcgccaac tatctgtggc agtggattgt ccaccgtagc
      781 gagcctgatt ggaacttcgc cgctgagcgc agctactttg aggccctcgc catattcttc
      841 tgctggataa tgtggcgatg agaatcagcg aacggccgtc gccgcgcaca gtctgcgtgt
      901 gagcgtggat ttgccgtagg cttgagggta agaccgggac aatcaccatg gaacagggta
      961 gtgatcaggg aaccaggacc gccgcgccat cgaaagcccc ggcacgcaaa tcggccaggg
     1021 cctgattggc ctgcagcagt ggataggttg tggtctgcgt ctcaatgtga aaatcagagg
     1081 cgtggcgcag gaattcaaat ccatcctgcc gcgtgagatt ggcaaccgag agcagttgcc
     1141 gttcttccca cagcaatcga taggcaaagg acggcagatc actcatatgg atgcccgcgc
     1201 atacgacccg tccgcctttg cgcacggcgc gcagcgcgag cggtaccagc tctcccaccg
     1261 gggcaaagag aatggcggcg tcaagtggtt cgggaggtgc ctgctcggat ccgccggccc
     1321 agaccgctcc gagttggcgc gcgaaggcct ggctgcgaag atcattcgcc cgggtgaagg
     1381 cgaacacctg tcgttcctga tgtcgcgcga cctgcgcaat aatgtgtgct gccgcgccga
     1441 agccatacaa accgagccgg gccccactgc cggccatttt cagggcgcgc caaccgatca
     1501 gtccggcgca cagcagcggt gcgagcgaca catcgctccc ggcctcccca agtggaaaga
     1561 cgtagcgcgc atcggcgatc accttgtccg caaagccacc atcgcgcgtg tggcccgtga
     1621 agaggggatg ctcacacaga ttctccctat ccgagtgaca gtagctgcag cgcccacagg
     1681 tgtggccaag ccaggacacg ccgacacgtt gcccggtatc cagatggtcc acgccggcgc
     1741 ccagcacctc gatgcgaccc acaatctcgt gaccgggaat gataggcagt tttgagtccg
     1801 gcaaatcccc gtcgaccaca tgcagatccg tgcggcaaac cccgcaggcg ctcacacgga
     1861 cgaggatctc attcggaccg gggatcgcat cttcaagctc gcaccagtca agcgcatgcc
     1921 cagtgcgtgt cagcagcatg gcgtgcatgc aagcactccc cgttaggcca tgaccggagg
     1981 cagtgctgga acaatgcggg ccccggtctg gatccgagcg acccgggtct tgaagcgcgg
     2041 gggtttgatg atgagcagat cgcaggtcac gcgatcgagc aggatttcag cggtgttgcc
     2101 aatcagcagg cccttcagac cggatctgga taccgctccc atcaccagca gatccgcatg
     2161 cagtttgcgc gccgcttgcg cgatgccgag ggcaggcggc gcgtcgatca gatgctgccg
     2221 cgcttgcccg attcgccggc cgcgcagcag tcgcgagaag cgcttgctgg ccgccgtacg
     2281 caaacgaggt gccagatcgc ggctcagctt tgggtacatg acgtcggtgg gatagctgac
     2341 gggcagcgtg gactcaaacg tgtgcagcgc gtgcagcgtt ccgcgcagcg cggtgctcac
     2401 ggtcgtggcc gcatccagaa tgcgttcatc gaggcgcgcg gacttgtccc agtagtgcgc
     2461 cggatccacg gccgccagcg tcaggggccg ggtatagaga ctgtctttgc ccaccagcag
     2521 tagcggcgcc gggcacagcc gcaacagttc ccaatcggcg tagcgcatca gggtgcgcgc
     2581 cagatgccgt ccggcgtgtc gatcgaccag taccagatcg gcgcgcgagg cggccgcctg
     2641 tcgaacgatg gcagcgtacg cgggccaatc ccaggccgcg gtgatcagcg tgcgcacgca
     2701 cttttgacct ggccgaacct ccagatcatg ggcgatgcgt tgcatctgct cggtgcgtcg
     2761 cttcatcaca tcgcgcttgg cgtccgtcag cgtccagtcc gagtaagtat agttgtccag
     2821 atagacaggc gtcgtgatcg catggaagag ttcaagcgtg gcgcccgtgg cgcgcgcgag
     2881 ctgcgcggcc ttgctggcgg cgggcgtcac accctgagcg gggtctttga cgctcacgag
     2941 aatgcggcga atgcagttca tacggttttc cttgatgggc gtggggtcga ctctcgatgg
     3001 cgcgatgcca cgtgtgagcc gagcgctagt cctgatggaa cgtacaggaa ccagcccacg
     3061 gcgccgtagg catcgtctat tcgaggggtc gctcgctcag agatttcgct ttggagagaa
     3121 gcgaaatcgg cgtcgctgcg cgggctgcgt gtactgcgct caggttggat gggtgtgtgc
     3181 atgagaactc gaaggcgggt cagagcgatg gttttacggt cagttggtcg gtgacgtgac
     3241 gcacccccgg aatgttccag gcggcgaatt cggcgtcgcc ccgctccggg agcgatcgta
     3301 ccgtgccctc caggactacc tcgccgtccg tgacgctgat gcgaatgttg ctggcgtcga
     3361 tgctcgcatg acggtgaaaa gccgcctgta tgcgatccct gatatcgtcc actttcacga
     3421 tcggctggat gtcaacctta tttgagatac caatgacgcc ccgcaccggg cgtagcgcgg
     3481 tttcgatcgc atcgcgctga aaccagttgg gaacgtgtcc cgtgagggtg atccaggcgc
     3541 cgctgacgat gagcttgaga tgttgcagcg gaacgtggat gttgttgcgc agaacgtgca
     3601 tcgcggcttc ggcaatatcc gcatccgagc ggctctggtc ggggtgatcc atgatgcgca
     3661 gctcattggc cagcacatgc acacccgcca cggactgggc tgcgtcctgc gccgcgtgcc
     3721 agtcggcgta ggagctgact tggccggtca gcgctaccac gccgtccttg acagagacgc
     3781 caatcttctg tgaatcaaag cgcggatccc aatcgagctg ggtctccaca tcctggcgta
     3841 ggcgcaaatc ggtactcatg cgaggctcct aattaatcca tttccggcgt tcactgtagc
     3901 gctcgcgtgg cgcgttgaca tacgcataaa ccacatcaca ggcgccggat ctggctatag
     3961 aagcaaagaa ggttgggtgc gcagcagtgc acgaagctga gtagtcagcc gcgccgtgcg
     4021 cgctcccagg cgtatgcgca agacccggtc cggtggccga agcccgtgct gttgctgcag
     4081 ccagccacgg gcttgttgga ggtcgtgaag atcgcccagg cagccctgca gttgcttgag
     4141 ccactctctg gtggaggcat ggtgggttgg cgggtagatt gccagtagat agcggcactt
     4201 ctttgtcatc aaccgaagtc gatgaatatc atcaggtgcc agaggagtgt tctttggtgc
     4261 gtgcctcagt cggctccagc gatcgagtaa cgcctcacgc agcagcgctt tggcattggg
     4321 cgagggctga gcggcccgcg tcagggtctt ggcggcccga tccatccgcc gcagacattg
     4381 atcaaatgcg gcggcattca gccagcggtg cgcccgagcg ctcgcctgtc ggctggcggc
     4441 tcgcagcagg acgagggtct gctggcgtcg atccgtgaca gggtgccgtt gtagcagcgg
     4501 gagcaggaga tccgtgcgca cccgcgcgtc gcgagcagcc ccgagatggt cgctgagggt
     4561 tttcaactca tggcgcaacg cacggcgtgc gcgtcctggc aatgccgggg ccagcgcgga
     4621 cagcgtgctg cgtagctgtc gcgccgcgat gcggctgtcg tgcacccgcg cagggcttga
     4681 tcggcgtcgc agtggcgcga gctgcgctcg cagcagccgg tattgctcat gcacaagacg
     4741 cctggtcgaa cgatgcgggt gccgatgcga gctcacagct tcaatgctcg ggcaacgccg
     4801 ggcgctgcgc atcaggagaa gtgcctattc gtcatttcgc gccagtcttg gagcatgaat
     4861 aagcccgtag cggccatcga ccgtgcgctt cgcaagcgtc tgcccagcga accgcgcgcg
     4921 ctacccttcg agcacgatca tcatgcgaac cagctgtgcc agggagctgg cttccatttt
     4981 ttccatcacc cgggcgcgat ggatttccac ggttcgctgg ctcacgccca gatctccggc
     5041 catgaccttg ttggccttgc cctgcgtgac cagatccagc acttcgcgtt cgcgatcggt
     5101 gagtgaattc agtcgcttct gcagcgactc ccgttcgcgc aactgacggc gattggcgct
     5161 gtcacgctcg atcgcagcct gcacacggtc cagcaggtcc tggtcgcgaa agggcttctg
     5221 tagaaagtcg aacgctccgt gttgtacagc ttccaccgcc atcggcacat cagcgtgccc
     5281 ggagatgaag atcaccggaa cgatcgcgcc gcgctcgttg agttggtcct gtagctccag
     5341 accactcatg cccggcatgc gaatgtcgag caccaggcag cctggccggt cgaacttgta
     5401 cgcggccagg aacgcggtgg cagacgcgta atccacgacc gtgcggccga cggacttgag
     5461 cagcatgcgc agcgagctgc gtaccgcgtc atcatcatcg accacgaata cggtgggttg
     5521 agcggaagct aaagtggggt tcataggatt cagaatccga catcagtgag gcagcgtcat
     5581 ggcgaagacg gcgccccggg gcgcattggg ctgatactcc agtcggccgc gatgagcttc
     5641 cacaatcgag cggctgatgg caagtccgag gccggtgcca tggatcttat gcgtgctgaa
     5701 tggcgtgaag agcttgggga ccatatccgc cgcaacaccc ggaccattgt cagctacgct
     5761 gaccgtgaca tcaccctgcg ggtccagcgc cgaacggatg tacaactcac gcggacccgc
     5821 gggtagcgtg gcgagcgcat cgacggcatt gcgaaacagg ttcagcaaga cctgctggat
     5881 ctggatccga tcaacgttca aaggaggcag atcactcaat agctgccact gcacctgtac
     5941 attgtgatcg cgcgcgtccg cccgagtgag atttccgatc tcccggatca actcgttgat
     6001 gtcggtgggc tcgcgccgcc cggcaggcgt cccgaccagc tttcgcaaat ggctgatagt
//
LOCUS       DATMRM010000085        10053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3447459, whole genome shotgun sequence.
ACCESSION   DATMRM010000085 DATMRM010000000
VERSION     DATMRM010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10053
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3447459"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..2271
                     /locus_tag="VNZ06_02565"
     CDS             268..2271
                     /locus_tag="VNZ06_02565"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HWW19665.1"
                     /translation="MGATQQERLLAPRIASILEQNHYRHITIDERLSPQVYDRFLTAL
                     DGQHSYFLASDIAGLDRWKNRFGDMLHTGDVDPAYAIFNVFQQRNRERMNYAIKLLET
                     EPDFTTDESFTFDREHAPWPATQADLDELWRLRVKNDAISLMLTGKSWQDTADLLRKR
                     YQRVLSRLDKITPEDVFETLMNSYCAVYDPHSNYFSPRSSEEYRIQMSLSYEGIGASL
                     ALTDDYVTVTNILAGGPAQADGSLKPNDRITAVGQGHDGPMTDVIGWRLDDVVQLIRG
                     KIDTVVRLQVLPAGATPGTPERTLELKRNRITLDNQAAKKEIRTIQRNGHAYKIGIIT
                     VPSFYNDMEAASNANNRSTTHDVHKLLQEISTAGGVDALVLDLRGDGGGYLPEAIGLT
                     HLFTNHGPVVQLRDTSGQLEVLKDPDSGPDYTGPLAVLVDRTSASASEIFAAAIQDFH
                     RGLIIGQTSFGKGTVQSVIPLDRWSSKPTEGQVTVTIGKFYRVTGESTQLRGVTPDIV
                     LPSPISTDDVGESVLEHALPWDRIASVPFQARVPEATLIQTLSTAETDRANHNADYQW
                     LLASLANLDSARKEKTLSLNLKKRQQERVTQDQARLTQENARRAADALPALKSIEDIV
                     ASEEPDVILAQASDIMTDEVAALDQNRNNQAVAQQSAPATSGH"
     gene            complement(2282..3304)
                     /locus_tag="VNZ06_02570"
     CDS             complement(2282..3304)
                     /locus_tag="VNZ06_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19666.1"
                     /translation="MKLIKQLGFLRLGGAPKQKLEVDDDRVLHLFRNRAELKKAHSEL
                     QDEVQRLKDRIKQQEGATARVQEMLQGLEARLTEPEAGYAALVFYQLRELWNLGRTLL
                     TQFVAELASQLEERERRAFFAEHNRKQFTIRQGVEANLREAELQGAAARDAVAQLEQQ
                     IARLQRFWHYFKRRSARQRLQAATLQSMLRMQDLDAARVARDALDAETPPEFGGLSID
                     ARRAINMAAIAYGQVLYERLARTPLMEMARDAAGRREPPREDYGDRARCEATMVEIVR
                     GRGLLQQKTSVTAEVKQRAERLRELAKYRNGEDTVPTADSLSSAVDGTKVLADDLWEI
                     FRVLLH"
     gene            complement(3359..3760)
                     /locus_tag="VNZ06_02575"
     CDS             complement(3359..3760)
                     /locus_tag="VNZ06_02575"
                     /inference="COORDINATES: protein motif:HMM:NF018552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HWW19667.1"
                     /translation="MSLYESNYLRFLHLAGEPLHLSGSLISQLGDGCELRLEVLERMP
                     YTTMLTLTYDVLPNGETARECLLNLRLRAYRDARLLEADEIRRPPIKENNLARRGPHP
                     GERELHQRWLRNMMLNKWLEYCAERGHRFTV"
     gene            complement(3916..4461)
                     /gene="orn"
                     /locus_tag="VNZ06_02580"
     CDS             complement(3916..4461)
                     /gene="orn"
                     /locus_tag="VNZ06_02580"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002053300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HWW19668.1"
                     /translation="MANHDYLIWIDLEMTGLMPDTDQIIEIATVVTDKDLNVIAEGPD
                     LAIHQPEEVLARMDSWNQRQHGGSGLLARVRASHMTLAEAERQTLLFLSTHMLPNASP
                     MCGNSICQDRRFLARLMPELEKFFHYRNLDVSTLKELARRWYPAIAKGYSKESAHLAR
                     ADIYESISELRYYREHLLVQP"
     gene            4503..5420
                     /gene="rsgA"
                     /locus_tag="VNZ06_02585"
     CDS             4503..5420
                     /gene="rsgA"
                     /locus_tag="VNZ06_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HWW19669.1"
                     /translation="MSSARVTATHRRALYVRDEAGRELPARFGRRELSIACGDLVQCE
                     FDAQHAELRVAAVEPRRNGLYRSNSRGGGELIAANLTQLLVVIAALPRPDYFVADRYL
                     CAAHCSGIAGIIVLNKRELPPPDDLQRELDAWRATGVAILEVSANTGHGLDPLKAQLS
                     GHISMLVGQSGVGKSSLLRALLPECDTAIGELIRDDEGRHTTTATRLYALPGGGDLLD
                     SAGVRDFAPAIDYLDPSALGFAEIESLAAHCRFGDCRHLREPGCAVRASVDVGGVNPR
                     RYESYRRLRRLYDRLRTQRSAAGERRFRP"
     gene            complement(5442..6041)
                     /gene="recR"
                     /locus_tag="VNZ06_02590"
     CDS             complement(5442..6041)
                     /gene="recR"
                     /locus_tag="VNZ06_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003254304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HWW19670.1"
                     /translation="MKHSPALAALIDALRGLPGVGPKSAQRMAFHLLQNDRQGAKSLA
                     NSLTASLESVGRCARCRMLTEGALCSICSNPQRDASLLCVVESAADVVAIEQSAGFRG
                     YYFVLMGHLSPLDGVGPEQIGVREFESLLDAGAVTEVILATNPTVEGEATAHFFSELV
                     RARGLTASRIAHGVPVGGELEYVDGGTLAHAMAGRHSLG"
     gene            complement(6046..6366)
                     /locus_tag="VNZ06_02595"
     CDS             complement(6046..6366)
                     /locus_tag="VNZ06_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019826710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HWW19671.1"
                     /translation="MANMNQFAKQAQALQANMQKAQAEIAALEVTGESGGGMVKVTMT
                     GRHEVRRVQIDPSVSSEDREMLEDLITAAVNDAVHRVESATQAKMQAAMGGLSLPPGM
                     KLPF"
     gene            complement(6376..7968)
                     /gene="dnaX"
                     /locus_tag="VNZ06_02600"
     CDS             complement(6376..7968)
                     /gene="dnaX"
                     /locus_tag="VNZ06_02600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWW19672.1"
                     /translation="MSAYTALARKWRPHSFAEMTGQEHVLRALANALESGRVHHAFLF
                     AGTRGVGKTTVARILAKCLNCECGVSANPCGQCASCREIDAGRSVDVIEVDAASRTRV
                     DDTRELLDNVQYAPTRSRYKIYLIDEVHMLSTHSFNALLKTFEEPPPHVKFLLATTDS
                     QKLPVTVLSRCLQFNLKRLSVALISSRLTEILKAEQLPFEPAAVQLLAQAGDGSLRDA
                     LSLLDQLLAFGNGKALEVDARAMLGTVARDHVLRAAEMLAAGDSAALLQFTRSLDEFS
                     PDYAQLLEQLAALLERVALKQLVPGYEGDELHPVELLSRLGQQIPAEDLQLYYQTAIL
                     GRRDLVLAPDARTGFRMTLIRMLAFRPVQSGLPAGAGTAPVTAPVPSPGGTMAHARGP
                     SGGSQPEATAASDWTAIVASLDVSAGAKQLANNCALLGREGAIVRLALDPRSAMMRTT
                     NLEEKLAQALSRHYGSPVRLEIELREAAPETPARAAERTEAQLRSQAQVAFEADPVVE
                     SFKQRFGASVLSDSVRPLKPNE"
     gene            complement(7985..8082)
                     /gene="ffs"
                     /locus_tag="VNZ06_02605"
     ncRNA           complement(7985..8082)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VNZ06_02605"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8101..8192)
                     /locus_tag="VNZ06_02610"
     tRNA            complement(8101..8192)
                     /locus_tag="VNZ06_02610"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8156..8158),aa:Ser,seq:gga)
     gene            complement(8277..8777)
                     /locus_tag="VNZ06_02615"
     CDS             complement(8277..8777)
                     /locus_tag="VNZ06_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19673.1"
                     /translation="MTICQTYPRARIGALLVCCALLPALSGCSVWNRVFHRHGTSGCS
                     AKPFALNSARRPVLKVPEGMSAPDTRNAIRIPDLSDHPERVRAKTEPCLAQPPDYFST
                     PLKLNLPARPPKPKHWWTFWRKNPAPPPASPTLMTPAPPAAPVPPAAPAPPTPPSEAP
                     ASSAPK"
     gene            complement(8774..9673)
                     /gene="dapA"
                     /locus_tag="VNZ06_02620"
     CDS             complement(8774..9673)
                     /gene="dapA"
                     /locus_tag="VNZ06_02620"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HWW19674.1"
                     /translation="MFSGSIVAIVTPMHADGSLDLAAWDRLLTMHLDAGTDGIVVCGS
                     TGEGPTVSESELMQLIERSRARLKDRAALIAGVGNNNTAVSVEAARRVSAAGVDALLV
                     NTPAYNKPSQEGLYRHCEAVAAASSVPVVLYNVPGRTAVDMLPATVARLMKLPRVVAI
                     KEAVPTMERVRELAALGGGRLAVLSGDDATAGQAMLNGARGVISVTANLVPKAMAEMS
                     AAALRGDRSLSEKLDAPLRALHEALFVEGNPIPLKWAMAQLGLIEGSLRLPLTALCES
                     LRPRVRAALDMALGAQLPQAKLA"
     gene            9857..>10053
                     /locus_tag="VNZ06_02625"
     CDS             9857..>10053
                     /locus_tag="VNZ06_02625"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWW19675.1"
                     /translation="MTTETMKQYLAVSAIGGDRPGMVHELTRVISDCGGNIAESRMAA
                     LGSEFAMLLLVNGNWHSLARI"
BASE COUNT         1840 a         3318 c         3083 g         1812 t
ORIGIN      
        1 gctgagccca tgatgatgcc gatcaaaacc gccatcggac gcaagtcagg cccctttttt
       61 tgcgacctgc ttggacagca tgtgcgctgc gactgccatc tggtacgcaa aaatgtcagt
      121 atggaggcag tgtagcggtt gtaaggtcac gaacgcagca tgaaacccag tgcacccctg
      181 gctctgactt gccttgcagt cctcgctctg gcggccaccg gctcctccgg ggcgcccgcg
      241 gcgactggtc tgcttccgga gggagtgatg ggtgccaccc agcaggagcg gctgctggca
      301 ccacgcatcg ccagcatcct ggaacagaat cactaccgtc acatcacgat cgacgagcga
      361 ctctcgccgc aggtctacga ccggttcctg accgcgctcg acggtcagca cagctacttc
      421 ctcgccagcg acatcgcagg ccttgaccgc tggaagaaca gattcggcga catgctccac
      481 accggtgacg tcgatccggc gtatgccatc ttcaacgtgt tccagcagcg caatcgcgag
      541 cgcatgaact acgcgatcaa attgctggag accgaaccgg atttcacgac cgatgagagt
      601 ttcacgttcg atcgcgagca cgcgccctgg cctgccacgc aggccgacct cgatgagctg
      661 tggcgcctgc gggtcaagaa cgacgcgatc tcgctgatgc tgaccggcaa gagctggcag
      721 gatacggcgg atctgctgcg caaacgctac cagcgggtgt tgtcgcgcct ggacaagatc
      781 acaccggaag atgtgttcga gacgctgatg aactcgtact gcgcggtgta tgacccgcac
      841 agcaactact tctcacctcg cagctccgag gaataccgca ttcagatgag cctgagctat
      901 gaaggcatcg gtgcctcgct cgcgctcacc gacgactacg tgacggtgac caacattctg
      961 gccggcggac ccgcgcaagc cgatggctct ctcaagccga atgaccgtat tacggcggtc
     1021 gggcagggtc acgacggacc gatgaccgac gtgatcggct ggcgactgga tgatgtggtg
     1081 cagctcatcc gcggcaagat cgacacggtc gtgcgtctgc aggtactacc ggccggcgcc
     1141 accccgggta cccctgagcg cacgctggag ctcaagcgca atcgcatcac gctcgacaat
     1201 caggcggcga agaaagagat ccgcacgatt caacgcaatg gccatgctta caagatcggc
     1261 atcatcaccg tgccgagctt ctacaacgac atggaagcgg ccagcaacgc caacaaccgc
     1321 agtaccacgc acgacgtgca caagctgctg caggaaatca gcaccgccgg cggcgtcgat
     1381 gcgctggtgc tcgacctgcg cggcgacggt ggcggctacc tgcccgaggc gatcggcctg
     1441 acccacctgt tcaccaacca tggaccggtc gtacagctgc gtgacaccag cggtcagctc
     1501 gaggtgctca aggatccgga ttccggtcca gactacaccg gcccgctggc ggtactggtc
     1561 gatcgcacca gcgcctcagc ctcggaaatc tttgcagccg ccattcagga tttccaccgt
     1621 ggactcatca tcggtcagac cagcttcggc aaggggaccg ttcagagcgt gatcccgctg
     1681 gatcgctggt cctcgaagcc gaccgaaggc caggtcacgg tcacgattgg taagttctat
     1741 cgtgtgacag gcgagagcac gcagctacgc ggcgtgacgc ccgacatcgt cctgccctcg
     1801 cccatcagca ccgacgatgt cggcgagagc gtgctggaac atgcgctgcc ctgggatcgc
     1861 atcgcctcgg tgccctttca ggcgcgcgtg ccggaagcca cgctgattca gacgctgtcc
     1921 accgctgaaa ccgaccgcgc caatcacaac gccgactatc agtggttgct cgccagtctt
     1981 gcgaacctcg acagcgcccg caaggaaaag accctatcgc tgaacctcaa gaagcgccag
     2041 caggaacggg taacccagga ccaggcgcgc ttgacgcagg agaatgcgcg ccgcgccgct
     2101 gacgcactgc cggcactgaa gtcgatcgag gacatcgtgg ccagtgagga accggacgtg
     2161 attctcgcgc aggcgagcga catcatgacc gatgaggtgg ccgcgctcga tcagaatcgc
     2221 aacaatcagg cggtcgcgca gcagtccgca ccggcaacgt ccggccacta gcgcgcgatt
     2281 gctagtgcag tagcacccgg aagatctccc acaagtcgtc tgccagcact ttggtgccgt
     2341 ccacggcact ggagaggctg tcggcggtgg ggaccgtatc ctcaccgttg cggtatttcg
     2401 ccagttcgcg caagcgctcg gcgcgctgct tcacttcggc ggtgactgaa gtcttctgtt
     2461 gcagcaagcc gcgtccgcgc acgatctcaa ccatggtcgc ttcgcagcgc gcacgatcac
     2521 cgtagtcttc acgcggcggc tcgcgccggc cggccgcatc gcgcgccatc tccatcagcg
     2581 gcgtgcgcgc caggcgctcg tacagcacct gtccgtaggc gatcgccgcc atgttgatcg
     2641 cgcggcgcgc atcgatcgac aggccgccaa attccggcgg cgtttccgca tccagcgcat
     2701 cacgcgcgac acgcgccgca tccagatcct gcatccgtag catcgattgc agcgtcgccg
     2761 cctgcaggcg ttgacgtgcg ctacggcgtt tgaagtagtg ccagaagcgc tgcagtcttg
     2821 cgatctgctg ttccagttgc gcgaccgcgt cgcgcgcggc agcgccctgc agctcggcct
     2881 cgcgcagatt ggcttccacg ccctgacgaa tcgtaaactg cttgcgattg tgttcggcga
     2941 aaaatgcccg ccgttcgcgc tcctcgagct gcgaggcgag ttccgctacg aactgcgtca
     3001 gcagtgtgcg accaaggttc cagagctcac gcagctgata gaacaccagc gcggcgtagc
     3061 cggcctccgg ctcagtgagt cgggcttcca gaccctgcag catttcctgc acgcgcgcgg
     3121 tcgcgccttc ctgctgcttg atgcgatcct tgagccgctg cacttcatcc tgcagctcgc
     3181 tatgggcttt cttgagctcg gcgcgattcc tgaaaagatg aagaacgcgg tcgtcgtcga
     3241 cctcgagctt ttgttttggc gcaccgccaa gtctcaggaa acccagttgt ttgatcagct
     3301 tcatcagtgg cgccatgaca ctcacgggcc accggtcgcc gatgcgtcct ggaagctcct
     3361 aaaccgtgaa ccggtggccc cgctccgcgc agtactccag ccacttattt agcatcatgt
     3421 tgcgcagcca gcgctgatgc agttcacgtt cccccggatg gggtcctcgg cgcgccaggt
     3481 tattttcctt aatcggcggc cttcgaatct catcggcctc gagcagccgt gcatcacgat
     3541 aagcgcgtag cctgagattc agcagacact cgcgcgccgt ctcgccgttg ggcagcacat
     3601 cgtaggtcag ggtcagcatg gtcgtatacg gcatgcgctc gagcacctcg aggcgcagtt
     3661 cacacccgtc gccgagctgt gaaatcaagc ttccggaaag gtgcagcggt tcacccgcca
     3721 ggtgcaggaa acgcaggtag ttgctctcgt acaggctcat caatgcgacg aagccgcgag
     3781 ggcgtgcccg ccaggagact ttaatgtccg tttcgcagag catggtccga ctatagcatg
     3841 ggccgatttc gggacaccgc cgtcaagcgg tgatttagtg ctgtaattcc cacttccggt
     3901 cgggtcaaaa ccgtcttacg gctggaccag caggtgctcg cggtagtagc gcaattcgct
     3961 gatcgattcg tagatatccg cgcgggccag atgcgccgat tccttggagt agcccttggc
     4021 gatcgctgga taccagcgtc gtgccaattc tttcagggta ctgacatcca ggtttcgata
     4081 atgaaaaaac ttctccagct ccggcatcag ccgtgccaga aaccgtcgat cctggcagat
     4141 gctgttgccg cacatgggag aggcgttcgg cagcatgtgg gtcgagagaa acagcaacgt
     4201 ctgccgctcc gcctctgcga gggtcatatg gctggcgcgc acgcgtgcca gcaagcctga
     4261 gccgccatgc tgacgctgat tccaggaatc catgcgtgcc agtacttctt cgggctgatg
     4321 aatcgcgaga tccggacctt cggcaatgac attgagatcc ttgtcggtga ccacggtggc
     4381 gatctcaatg atctgatccg tgtccggcat caggccagtc atctccaggt cgatccaaat
     4441 caggtaatca tgattggcca tgacgccacg tttccacacc agttcgggca tcatagcaag
     4501 gaatgtcctc agcccgagtt accgccaccc atcgtcgtgc gctgtacgtg cgcgatgagg
     4561 ccggccgcga gctgccggcc cgcttcgggc ggcgggagct gtcaattgcc tgcggcgatc
     4621 tggtgcaatg cgaattcgac gctcagcacg ccgaactgcg cgtcgcagcc gtggaaccgc
     4681 gtcgcaatgg cctgtatcgc agcaattcgc gcggtggcgg cgagctgatc gcagccaatc
     4741 tgacccagct gctggtggtg atcgcagcac tgccgcgccc ggattatttc gtcgcggacc
     4801 gctatctgtg tgcggcccat tgcagcggca tcgcgggaat catcgttctg aacaagcgcg
     4861 agctgccgcc gcccgatgac ctgcagcgcg aactcgacgc atggcgtgcc accggcgtcg
     4921 ctattctgga ggtttcggcg aataccggac acggactcga tccgctcaag gcacaactat
     4981 ccggtcatat ctcgatgttg gtgggacagt ctggtgtcgg caagtcgtcg ctgctgcggg
     5041 cgctgctgcc ggagtgcgat accgcgatcg gagaactgat ccgcgacgat gagggccgcc
     5101 acaccaccac ggccacccgg ctgtacgcac tgccaggcgg tggcgacctg ctggactctg
     5161 cgggcgtgcg cgactttgcg ccggccatcg actatctgga cccgtcggcg ctcgggtttg
     5221 cggagattga gtcgctggca gcgcactgtc ggtttggcga ctgccggcat ctgcgcgaac
     5281 cgggctgcgc ggttcgcgcc agtgtggatg tcggaggcgt aaacccccgg cgctatgaga
     5341 gttaccgtcg cctgcgccgt ctgtacgatc gcctgcgcac gcagcgcagc gccgcgggcg
     5401 aacgccgctt cagaccctag agtcgtcgac acgctcggcc attaacccag cgagtggcgg
     5461 cccgccatcg cgtgtgccag cgtgccgccg tccacgtact ccagctcccc gcccaccggc
     5521 acgccatgcg cgatgcggct ggcggtcaaa ccgcgggcac gcaccaactc ggaaaagaaa
     5581 tgtgccgtgg cttcgccctc gacggtcgga ttggtggcca ggatcacctc ggtgacggcg
     5641 ccggcatcca gcagcgattc gaattcgcgc acaccgatct gctcgggccc cacgccatcc
     5701 agcggtgaca gatgacccat caggacgaag tagtatcccc gaaatcccgc cgactgttcg
     5761 atcgccacca cgtcagctgc cgactccacc acacacagca atgaggcatc gcgctgtgga
     5821 ttggagcaga tcgaacacaa cgcgccttcg gtcagcatgc ggcaacgggc gcagcgcccc
     5881 accgactcca gagacgcggt cagcgaattc gccagactct tggcgccctg gcggtcgttc
     5941 tgcagaagat gaaacgccat gcgctgcgca ctcttcggcc ccacgcccgg caatccgcgc
     6001 agcgcgtcga tcagggcggc gagcgcgggc gaatgcttca tcgctctaga atggcagctt
     6061 catgccgggc ggcagcgaca gaccacccat cgccgcctgc attttcgcct gcgtcgcgct
     6121 ctcgacccgg tgcaccgcgt cgttgacggc ggcggtgatc aggtcctcga gcatctcgcg
     6181 atcctcgctg ctcaccgagg ggtcgatctg cacgcgccgc acttcatggc gacccgtcat
     6241 ggtcactttg accatgccgc cgccggattc tccggtcact tcgagcgcgg cgatctcggc
     6301 ctgcgccttc tgcatgttgg cttgcagcgc ctgcgcctgt ttggcgaatt gattcatgtt
     6361 ggccatggac ctacctcact cgttcggttt taacgggcgc acggaatccg agagcacgct
     6421 cgctccgaag cgctgtttga aactctctac cactggatcg gcttcgaaag cgacctgcgc
     6481 ctgcgaacgc agctgagcct cggtgcgctc ggccgcgcgc gccggagtct ccggtgccgc
     6541 ttcgcgcaat tcgatctcga gcctcacagg cgagccgtag tgacgcgaca gcgcctgggc
     6601 cagtttctcc tcgagattgg tggtgcgcat catcgcgctg cgcgggtcga gcgccagacg
     6661 cacgatcgcg ccctcccggc caagcaacgc gcaattgttg gccagctgct tcgcgcccgc
     6721 gctgacatca agggaggcga cgattgcggt ccaatccgaa gctgcggtgg cctctggctg
     6781 actgcccccg gaagggcctc gcgcatgcgc catcgtgccg cccggtgacg gcaccggtgc
     6841 cgtcaccggt gcggtgcctg cgcctgccgg caggccgctc tgcaccggac gaaaggccag
     6901 catgcgaatc agcgtcatac gaaaaccggt gcgcgcatcc ggtgccagca ccaggtcgcg
     6961 gcgccccagg atcgccgtct gatagtagag ctgcagatct tccgcgggaa tctgctgtcc
     7021 caaacggcta agcagctcca ccgggtgcag ctcgtcgcct tcatagcccg gcacgagctg
     7081 tttgagcgcg acgcgctcga gcagcgctgc gagctgctcc agcagctgtg cgtagtcggg
     7141 ggagaactca tccagtgatc gcgtgaactg cagcagcgcg gccgaatcac ccgccgccag
     7201 catctctgcc gcgcgcagca catgatcgcg cgccaccgtg cccagcatcg cgcgcgcatc
     7261 gacctccagc gccttgccat tgccgaatgc cagcaactga tcgagcaatg agagtgcatc
     7321 gcgcaggctg ccgtcgccgg cctgggccag cagttgcacc gccgccggtt cgaatggcag
     7381 ctgctctgct ttgaggatct cggtcaggcg cgaactgatc aaggcgaccg acaggcgttt
     7441 cagattgaac tgcaggcagc gcgacagcac cgtcaccggc agcttctgcg aatccgtggt
     7501 cgcaagcagg aacttgacgt ggggcggtgg ctcctcgaac gttttcagca acgcgttgaa
     7561 ggagtgcgtg gacagcatgt gcacttcgtc gatcaggtag atcttgtagc gactgcgcgt
     7621 cggcgcgtat tgcacgttgt cgagcaactc gcgcgtgtcg tctacccgcg tgcgcgaagc
     7681 cgcatcgacc tcgatcacat cgacgctgcg gccagcatcg atctcacggc aactcgcgca
     7741 ttggccacac ggattggcgc tgacgccgca ttcacagttc aggcatttgg ccaggatgcg
     7801 cgcgaccgtg gtcttgccaa cgccgcgggt accggcaaac aagaacgcgt gatgcacgcg
     7861 cccgctctcc agcgcattgg cgagcgcgcg cagtacgtgc tcctggccgg tcatttcggc
     7921 aaaactgtgc ggccgccatt tgcgggccag tgcggtgtaa gcgctcatac ctttgggagg
     7981 tgatccgtac cagcctgaac ttcggcaccc gactgcgccg ttaccgttgc tcccttccgg
     8041 gcctggcggg attcacggtt agtcgtcgcg aaggaaccgg cacggatcac cgtggaccgt
     8101 tggcggagag ggtgggattc gaacccacga acacccgtga agatgttact ggaattccag
     8161 tccagcgcct tcgaccgctc ggccacctct ccattcaatg cgttacgatg aatatttgaa
     8221 gctgatgatg acacctggtc ggcaaattct agcaaccgtg cggcacgcgc gcgcggctac
     8281 ttcggcgcac tggacgcagg agcctcagac ggtggagtcg gcggtgcagg cgccgcagga
     8341 gggactggcg cggccggtgg cgcgggcgtc atcagcgtcg gtgacgccgg cggcggcgca
     8401 gggttcttgc gccagaacgt ccaccagtgc ttcggcttcg gcgggcgcgc aggcaggttc
     8461 agcttcagcg gcgtcgagaa ataatccggg ggctgcgcca ggcaaggctc ggtcttggcg
     8521 cggacacgct ccggatgatc ggacagatcg gggatccgga tcgcattgcg ggtatcgggc
     8581 gcactcatgc cttcggggac cttgagcacc gggcgcctgg cgctgttcaa cgcgaagggt
     8641 ttcgcgctgc agccgctcgt cccgtgtcgg tggaacacgc ggttccacac cgagcagccg
     8701 gacagtgccg gcagcagcgc gcagcacacc aacagcgcgc cgatccgggc gcgcgggtac
     8761 gtctgacaga tagtcacgcc aatttcgcct gcggcagctg cgcgcccaat gccatatcga
     8821 gcgcggcgcg gacgcgcggc cgcaagcttt cgcacagcgc agtcagcggc aggcgcaggc
     8881 tcccttcgat cagtcccagc tgcgccatcg cccacttcag cggaatggga ttgccttcga
     8941 cgaacagtgc ctcatgcagc gcacgcagcg gtgcatccag tttctcagac agcgaacgat
     9001 cgccgcgcaa cgccgcagcg ctcatttccg ccattgcctt gggcaccaga ttcgccgtca
     9061 ccgaaatcac tcctcgtgca ccgttgagca tcgcctggcc tgccgtggcg tcgtcgcccg
     9121 acagaactgc cagtcgcccg cccccgagtg ctgccagttc gcgcacccgc tccatcgtcg
     9181 gcacggcttc tttgatcgca accactcgcg ggagtttcat caggcgcgcg actgtcgccg
     9241 gcaacatatc caccgcggtg cgtccgggga cgttgtagag caccaccggg acactggagg
     9301 ccgccgcgac cgcttcgcag tgccggtaca aaccctcttg agagggcttg ttgtacgccg
     9361 gtgtgttgac cagcagggcg tccacccccg ccgccgatac gcgtcgggcg gcctccacgg
     9421 acacggccgt gttgttgttg ccgaccccgg cgatcagcgc cgcccgatcc ttcaggcgcg
     9481 ctcggctgcg ctcgatcagc tgcatgagtt cggattcact gacggtcgga ccctcacctg
     9541 tcgagccgca gacaacaatg ccgtccgtcc ccgcatccag gtgcatcgtc agcagtcggt
     9601 cccacgcggc aaggtccagg ctgccatccg catgcattgg cgttacgatc gcaacgatac
     9661 tgccggaaaa catctgacct gctctatttt attgaatcga tatggcatgt tacgggccgg
     9721 cgccaaccat tagcaagcaa ggaggccgtc tgagcaatcc gcgtgagtca acccttcggg
     9781 agcgggtggg tttgctcgga cagcctcccc gcatacgcaa gcagcgccag taccggtaca
     9841 ctgccaatcc actcctatga ccaccgagac catgaagcag tatcttgccg ttagcgccat
     9901 cggcggcgac cgacccggca tggtccacga attgacgcga gtcatcagtg actgcggcgg
     9961 caacattgcc gagagccgca tggcggcgct gggctcggaa ttcgcaatgt tgctattggt
    10021 gaacggcaac tggcactcgc tggcgcgcat cga
//
LOCUS       DATMRM010000086         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_965711, whole genome shotgun sequence.
ACCESSION   DATMRM010000086 DATMRM010000000
VERSION     DATMRM010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965711"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..137
                     /locus_tag="VNZ06_02630"
     CDS             9..137
                     /locus_tag="VNZ06_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19676.1"
                     /translation="MIYGFVASYLILKIIDWTIGLRVPEEQEREGLDITLHGESLE"
     gene            498..>2172
                     /locus_tag="VNZ06_02635"
     CDS             498..>2172
                     /locus_tag="VNZ06_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HWW19677.1"
                     /translation="MASHWLIETAISSLNRLTHRGAIAADGKTGDGCGLLLKIPTLFL
                     RAAAVEAGLQLASMFAAGLVFLNRDFVKAAHSRDVLTRELLRENLSVAGFRDVPTDPA
                     ACGAEALKTLPAIVQVFVNAPGDIDAATFNRKLFLARRRSEKKLEALDPVFYVPSLSS
                     NTIVYKGMVMPQFLTQFYPDLKDVRMQASVAVFHQRFSTNTLPQWRLAHPYRYLAHNG
                     EINTIQGNRSWALARGPLLRSPVLPDLSDILPIVSLKGSDSQSLDNMLELLLAGGLDP
                     MHALRMLIPPAWQSVDMIDPDLKAFYEYYATHMEPWDGPAGIVVTDGRYACCTLDRNG
                     LRPARWVITKNRHITIASEAGVWDYLPEDVVRKGKLGPGEILALDLQTAELMDTKQVD
                     DLLKSRHPYKVWLKKGVRYLQTDLIDPRLAAEPFDRDTLALFQKMYNVSAEERDEIIR
                     VLAEDESEAVGSMGDDTPMPVLSKKVRSLYDYFRQQFAQVTNPPIDSLRESIVMSLQT
                     QIGPEANIFVPAQQHAEQIVLSSPILSQRKLRQIMGLEDSGVPHEFIDLQ"
BASE COUNT          428 a          648 c          677 g          419 t
ORIGIN      
        1 cgacgacggt gatctatgga tttgtcgcga gctacctgat tctgaaaatc atcgactgga
       61 cgatcggcct gcgcgtaccc gaggaacagg agcgtgaggg cctcgacatc actttgcacg
      121 gggaaagtct ggagtgaaac cgcccgccgt tggtcatccg gcggctgcac ggcaggcccg
      181 gactttcgga tcggtgcgcg cgtgccgccg attgacgctg tgcgcggccc tagtatcatg
      241 gccttcatcc ggcgcgtgtc agtgttcgcc ccaagaactt gcagatgaac gttttataag
      301 gctctgcgga gaaaatccgt gaagtatggg gcggcacggg caacggaatg gtagacggta
      361 gcaagaacgc tattgcgggt aggagcgcgc gccttttgga aggcgatgca gatccgcgcg
      421 cgcaggattg gggcctttac cggcaatcct acgaaaaaga ttcctgtggc tttggcctga
      481 tcgcgaatct cgacgatgtg gccagccact ggctgatcga aacagcgatc agctctttaa
      541 atcgcctgac gcatcgtggc gcaattgcgg ctgatggcaa gacaggggat ggctgcgggc
      601 tgctgctcaa gatcccaacg ctattcctgc gtgcagctgc agtcgaggca ggcttgcagc
      661 ttgcctcgat gttcgcggcc ggactcgtct tcctgaaccg ggactttgtc aaggccgccc
      721 acagcaggga tgttctgacc cgcgagcttc tgcgggagaa tctgtcggtc gccggttttc
      781 gtgacgtacc gacagatcct gccgcctgcg gtgcagaggc gctgaagacc ctgccggcga
      841 tagtgcaggt gtttgtcaat gcaccgggcg acatcgacgc agccaccttc aatcgaaagc
      901 tgttccttgc gcgccggcgc agcgaaaaaa agctggaagc actggacccg gtgttctacg
      961 tgccgtcgct gtcctcgaat acgatcgtct acaagggcat ggtgatgccg caattcctga
     1021 cgcagttcta tccggacctg aaggatgtgc ggatgcaggc ctcggtggcc gtatttcacc
     1081 agcgattctc aacaaacacg ctgccgcagt ggcgactcgc gcacccgtac cgctacctcg
     1141 cccacaacgg cgagatcaac accatccagg gcaatcgaag ctgggccctg gcgcgcggcc
     1201 cgttgctgcg ctcgccggtg ttgccggact tgagcgacat cctgccgatc gtctcgctta
     1261 aaggatccga ttcgcagtcc ctcgacaaca tgctcgaact gctgctggcg ggcggactcg
     1321 atcccatgca tgcgctgcgc atgctgatcc cgcctgcgtg gcagtcggtg gacatgatcg
     1381 atcccgactt gaaggcattc tacgagtact acgccaccca catggagccg tgggacggac
     1441 ccgccggcat cgtggtgacc gatgggcgct atgcctgctg tactctggac cgcaacgggt
     1501 tgcgtcccgc gcgctgggtc atcaccaaga accgccacat caccatcgca tccgaggcgg
     1561 gggtctggga ctatctcccc gaggacgtgg tgcgcaaggg caagttgggg cccggcgaga
     1621 tcctggccct cgacctgcag accgccgagt tgatggacac caagcaggtc gatgatctgc
     1681 tcaagagccg tcatccctac aaggtgtggt tgaagaaggg ggtgcggtat ctccagacgg
     1741 acctcatcga tccgcgcctg gcggccgagc cctttgaccg ggacaccttg gcgctgtttc
     1801 aaaagatgta caacgtgtcg gccgaggagc gcgatgagat catccgcgtg ctggccgaag
     1861 atgagagcga agcggtggga tcgatgggcg acgacacgcc gatgccggtg ctgtcgaaga
     1921 aggtgcgttc tctttacgac tacttccgcc agcagttcgc ccaggtgacg aacccgccga
     1981 tcgactcgct gcgcgagagc atcgtcatgt cgctgcagac gcaaatcggc ccggaagcca
     2041 acatcttcgt gccggcacag cagcacgccg aacagatcgt gttgagttcg cccatcctgt
     2101 cgcagcgaaa attgcgccag atcatgggcc tcgaagattc gggcgtgccc cacgaattca
     2161 ttgatctgca at
//
LOCUS       DATMRM010000087         6095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1894125, whole genome shotgun sequence.
ACCESSION   DATMRM010000087 DATMRM010000000
VERSION     DATMRM010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894125"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VNZ06_02640"
     CDS             <1..981
                     /locus_tag="VNZ06_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWW19678.1"
                     /translation="GSAQKYLSGTYLGRRTAGVQAALGCRFRCTFCGVAAMFRGKTAM
                     PAPERLELQLRHLTSRFGVDSLIFYDHNFFDREEDTIPILEVLAKFGLPWWCFARSDA
                     MVNLSARSWELVRKSRLRMAYIGAESPSDWMLHEVRKGTKTDQTLEAVEVCRSHGVTP
                     ELSFMLAPPQDPEGETERTFEFIRHVKRMHPKTEIMLYIYAPMPPAPGSKNPQVERSI
                     ANLKDANGEPLRFPTTADGWAQPDWVTYWCHTDVPWLTPRLRSRIQDFTTVLGCRFPT
                     MTDIRSPGWGKMTLRTLAAWRYRLRRYERPWELNFSKRFIQLRDPKLTGL"
     gene            1315..2343
                     /locus_tag="VNZ06_02645"
     CDS             1315..2343
                     /locus_tag="VNZ06_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19679.1"
                     /translation="MNHRSRTVKTDQPPDPFHERTHPLLQVVRPVMGAKITFESDSRR
                     LLELVRVTYDGLPQHRLPGPQARLRIRLIERDADRDSGTRRGEPPELAMFSGAGWLGG
                     VSKGSDMVIISPRERSALVAVSPQTLRSPYHTRYELIEFAVFTLAARSQRLSPLHSAC
                     VGKSGRGVLLMGPSGSGKSTVTLMAVMSGLEFLSEDAVFVAPGALLATGVSNYLHVRT
                     DSLHWLSPAQRRLLRASPVIRRRSGVSKFELDLRRGGYRLASSALKITAVVFLSARSA
                     AGRDLLRPLSRTALRSTFAANQAYAVNQPNWPAFSTRLARFPAYELRRGRHPDESVEA
                     LRSLLPHR"
     gene            complement(2340..3137)
                     /locus_tag="VNZ06_02650"
     CDS             complement(2340..3137)
                     /locus_tag="VNZ06_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19680.1"
                     /translation="MRSARRAGERTLRCSLDLAKSETTVSLEDEGWCWRDQVYPYPQG
                     LKERTIYLWDGHFEPVARFSTSLIKLIPTHWGPPTFEIDGIKMLPSEHVSPMADARRK
                     VGLVEPRGKRILDTCGGLGYFAACALDAQASRVLSFEVNPDVLWLRSLNPWSPDHRWA
                     KPHADLAMVSADVSAGLESLPSASFDAILHDPPRFGIAGELYSQSFYGQLARVIKPRG
                     RLFHYTGSPNKLTTHRDLAREVAARLRRAGFCVDVIGDGLLAQAPAR"
     gene            3194..3772
                     /locus_tag="VNZ06_02655"
     CDS             3194..3772
                     /locus_tag="VNZ06_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19681.1"
                     /translation="MPETIAYMKGWENFYVITGSAAAGLTGLTFVVIALASDANMVSL
                     TGLRAFITPIVIHFGAVLWIASVLSIPGHTALSLEITLILTALALTVYSVVTVRRMVA
                     NMKQYVPAMQDWIWNATLPWLCCLALLSAGPMVTRHPVEALYLVGIVTLAMLFIGVHN
                     AWDLAVWITAERPGRQREQSPPRAAPPPDKPT"
     gene            complement(3796..5157)
                     /gene="rimO"
                     /locus_tag="VNZ06_02660"
     CDS             complement(3796..5157)
                     /gene="rimO"
                     /locus_tag="VNZ06_02660"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HWW19682.1"
                     /translation="MSSQRKASVRRRAPAVGFVSLGCPKALVDSERLITQLRSRGYVI
                     APGYDNADLVVVNTCGFIDAAIDESLDAIGEALRKNGRVLVTGCLGANPQRILERHPK
                     VLGVTGPHAYERVLEAVNQYLPQPHDPLVDLVPPQGIRLTPRHYAYLKISEGCNNRCS
                     FCIIPSLRGRLASRALDDVLREAEALVRAGVKELLVISQDTSAYGSDIRYRAVNWREQ
                     AYESRFIDLARSLNTLGVWVRLHYVYPYPHVDEVVPLMNERGVLPYLDIPFQHASPSV
                     LKRMRRPAHAQDTLQRIRSWRAARPELTLRSSFIVGFPGETDAEFEALLQWLDAAELD
                     RVGCFKYSPVEGAAANGIAQAVPEAVKQERYQRFMQKAGEISAARLARRIGTRCRVLI
                     DAIDAGIATGRTMGDAPEIDGVVRIRHPEGLRAGEFAQVELTGADSYDLTARLLSSQE
                     SDA"
     gene            5180..5761
                     /locus_tag="VNZ06_02665"
     CDS             5180..5761
                     /locus_tag="VNZ06_02665"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWW19683.1"
                     /translation="MPFDRDYYRRFYFNPQTAVTSRAEMRARARLIVGCVNYVGLPVG
                     SILDAGCGVGLLRAPLLRAFRRADYTGVEVSQYLCQRYGWHCSPVEKFRSRRRFDLVV
                     CYDVLQYLAEDGARCAIDNLARLCRGALYFGALTTEDWRENCDQSRTHRVAGLRLGSW
                     YRRELSRSFRPLGCGMWLKKELPLTVWNLDAVA"
     gene            5860..>6095
                     /locus_tag="VNZ06_02670"
     CDS             5860..>6095
                     /locus_tag="VNZ06_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19684.1"
                     /translation="MEVLFSGATVSLPQSLHDVRVLQLQTGSQTPRKYRIESREMSVE
                     LQARGMQMHRAAAAQLFDAVPPARVPWGIRAGWV"
BASE COUNT         1063 a         1894 c         1972 g         1166 t
ORIGIN      
        1 ggcagtgcgc agaagtatct ttccggcacc tatctgggac gtcggacggc aggggtccag
       61 gcggccctgg gatgccgctt ccgttgcacc ttctgtggcg tcgccgccat gtttcgcggc
      121 aagaccgcga tgccggcgcc cgagcggctg gagcttcagc tgcgacatct gacctcgcga
      181 ttcggtgtcg attcgctgat tttctacgac cacaatttct tcgatcgtga ggaagacacc
      241 attccgatcc tggaagtgct ggccaagttt ggtcttccgt ggtggtgctt cgcgcgctcg
      301 gatgcgatgg tgaacctgtc cgcgcgctcc tgggaactgg tgcgcaagag ccggctgcgc
      361 atggcgtaca tcggcgcgga atcgccgagc gattggatgc tgcacgaagt gcgcaagggc
      421 accaagaccg accagacatt ggaagcggtc gaggtctgcc gcagtcacgg cgtcacaccc
      481 gaattgtcct tcatgctggc gccgcctcag gatccggaag gcgagaccga gcgcaccttc
      541 gagttcattc gccacgtcaa gcgtatgcat ccgaagaccg agatcatgct gtacatctac
      601 gcgccgatgc cgcccgcacc gggtagcaag aatcctcagg tcgagcgctc gattgccaat
      661 ctgaaggacg ccaacggcga gccgctacga tttccgacca ccgcggacgg gtgggcccag
      721 cccgattggg tgacctactg gtgccacacg gacgtgcctt ggctgacccc acgactgcgc
      781 agccgtatcc aggacttcac gacggttctc ggttgccgct tccccaccat gacggatatc
      841 cgctcaccgg gctggggcaa gatgacgttg cgaacgctgg cggcctggcg ctatcgcctg
      901 cgtcgctacg aacgaccttg ggagctcaat ttttcgaaac gcttcatcca gctgcgggat
      961 ccgaagctca caggtctcta gctgatcagt caatcgtcag gcgggcgtcc cgagcccggc
     1021 ggtcccgccg ctgagcttgt atcaggtgtg ctgcggacca ggtgcttgcg cagcacgcgt
     1081 gcgaagcgcg cgcgcgcgag atccgatggc ccatagaagg cccggtcccc gacggcgttg
     1141 cgtgagggtt gccatcggtt ggatgccggc ttgacgaagc gtcgcgacga agttaacaag
     1201 gcgcgtctcg gaaaaaacgt aactcgcgcg tatgtgacac caacgcggcg cacggttgta
     1261 agcaatgatg cgaattggtg gcgtccgaag tcatttgcac cctgtagcga agtcatgaat
     1321 caccgatccc gtaccgtaaa gaccgatcag ccacccgatc cattccatga gcgcacgcac
     1381 ccgttgctgc aggtcgtgcg gccggtcatg ggagcaaaaa taacctttga gagcgacagc
     1441 aggcgcctgc tggagctggt gcgtgtgacg tatgacggct tgccgcagca tcggcttccc
     1501 ggaccacaag cgcgccttcg catccgcctg atagagcgcg atgccgatcg ggatagtggc
     1561 acgcggcgcg gcgagccgcc tgagctcgcg atgttttcgg gcgcaggctg gttgggagga
     1621 gtctcgaagg gctcagatat ggtcatcatc tcgccacgcg agcgctcggc gctggtggcg
     1681 gtatcgcctc agacccttcg atcgccctac cacacgcgct acgaactgat cgagttcgcg
     1741 gtcttcacac tggccgcgcg ttcgcagcga ttgagtccgc tgcattccgc ctgtgtcgga
     1801 aaatcggggc gcggcgtgct cctaatgggc ccgagcgggt ctggaaagtc gaccgtaacc
     1861 ctgatggcag tcatgagcgg cctcgagttc ctgtcggagg acgcggtatt tgtagcgccg
     1921 ggtgcgctgc tcgctacggg ggtttccaac tatctgcatg ttcggaccga cagcctgcac
     1981 tggctctccc ctgcccagcg ccgtttgctg cgggcatcgc cggtcatccg ccgccgcagc
     2041 ggtgtcagca agttcgagct ggatctgcgg cggggcggct accgcctcgc gtcctcggcc
     2101 ctgaagatca ctgcggtcgt tttcctttcg gcgcgtagcg ccgcgggtcg ggacttgctc
     2161 aggccgctgt cgaggacggc gctgcggtcc acattcgccg ccaatcaggc ttatgccgtc
     2221 aaccaaccca actggccagc attcagtacc cgcctcgcga gatttccggc gtatgaactg
     2281 cgccgcggac gtcacccgga cgagtcagtg gaggcactgc gctcgctgtt gccgcaccgc
     2341 tagcgagccg gtgcctgcgc cagcaatccg tcgccgatca cgtcgacgca gaatccggcg
     2401 cgcctgaggc gggcagcgac ttcgcgtgct aaatcgcgat gggtggtgag cttgttcggg
     2461 ctcccggtgt agtgaaacag gcgtccgcgc ggcttgatga cgcgcgcgag ctgaccatag
     2521 aacgactgcg agtagagctc accggcgatg ccgaatcgcg gtgggtcgtg cagaatcgca
     2581 tcgaaagagg cgctcggcag actctcgagc cctgcgctga catcggcgct caccatcgcc
     2641 aggtctgcat gcggctttgc ccagcggtga tccggtgacc aggggttcag gctgcgcagc
     2701 cacaggacgt cagggttgac ctcgaacgag agcactcggg atgcctgcgc atccagtgca
     2761 caggccgcga agtagccaag tccgccgcag gtatcgagga tgcgcttgcc gcgtggttcg
     2821 accagaccca ccttgcgtcg tgcatcggcc atcggcgaaa catgctcgct cggcagcatc
     2881 ttgatgccgt cgatttcgaa cgtgggcggt ccccagtggg tggggatcag cttgatcagc
     2941 gaggtgctaa aacgcgccac cggctcgaaa tgcccgtccc agaggtagat cgtgcgttcc
     3001 ttcaatccct gggggtaggg atacacctgg tcgcgccagc accaaccctc atcttccagt
     3061 gaaaccgtgg tctcggactt tgccagatcc agcgagcagc gcagcgtgcg ctctccggcc
     3121 ctcctggccg accgcatggc agtttgaacg gacaatgtta gtagcggacc catggtgtcg
     3181 ttagaattga tgtatgccag agaccattgc atacatgaag ggctgggaga acttctacgt
     3241 catcaccgga tcggctgcgg ccgggctgac gggtctcacc ttcgtcgtga tcgcgctcgc
     3301 ctccgatgcc aacatggtga gcctgaccgg cttgcgggct ttcatcaccc cgatcgtgat
     3361 tcatttcgga gccgtgctgt ggatcgcttc cgtcctgagc attcccggac acaccgcgct
     3421 cagtctcgag atcacgctga tcctgacggc actcgcgctg accgtctata gcgtggtgac
     3481 ggtgcgtcgc atggtcgcca acatgaagca gtacgtgccc gccatgcagg actggatctg
     3541 gaatgccact ctgccctggt tgtgctgcct ggcgttgttg tcagcaggtc cgatggtcac
     3601 gcgtcatccg gtggaggcac tctacctggt cggcattgtg accctcgcga tgctgttcat
     3661 cggggtacac aacgcctggg acctggctgt gtggatcacc gccgaacgcc cgggtaggca
     3721 gcgcgagcaa tcgccgccgc gtgcggctcc tccgccggat aagccaacct agagcctcag
     3781 ggcggcgagc cgcagtcagg cgtcggattc ttgggaactg agtagccggg ccgtcagatc
     3841 gtagctgtcg gcaccggtga gttcgacctg cgcaaattcc cccgcgcgca gtccctccgg
     3901 atggcgtatt cgcaccacgc cgtcgatttc tggcgcgtcc cccatggtgc ggcccgtggc
     3961 aatgccagcg tcaatcgcgt cgatcagcac ccggcaacga gtgccgatgc gccgcgcgag
     4021 tcgggcggca ctgatttcgc cggccttctg catgaagcgc tgatatcgct cttgtttgac
     4081 cgcttccggg acggcttggg cgatgccgtt agcggcagcg ccttcgaccg gcgaatactt
     4141 gaaacacccg acgcggtcca gctcggcggc gtcgagccac tgtagcagcg cttcgaattc
     4201 tgcgtcggtc tcgcctggaa agccgacgat gaagctactg cgtagcgtca attccgggcg
     4261 cgccgcgcgc cacgaccgga tgcgctgcag cgtgtcttgc gcgtgagccg gacgccgcat
     4321 gcgcttcaga acggagggac tggcatgctg gaatggaatg tccagatagg gcaacacgcc
     4381 acgctcgttc atcagcggga cgacctcatc cacgtggggg tacgggtaga catagtgaag
     4441 tcgcacccac acaccgagtg tgttgagcga tcgcgcgaga tcaatgaaac gcgattcgta
     4501 ggcctgctcg cgccagttca ccgcgcgata gcggatatcg ctcccatagg cgctcgtgtc
     4561 ctgcgaaatg accagcaact ctttgacccc ggcgcgtacc agcgcttcgg cttcgcgcag
     4621 tacgtcgtcg agtgcgcgac tggccaatcg tccgcgcagc gacggaatga tgcagaagct
     4681 gcaacggttg ttacagccct cggagatctt cagataggca tagtggcgcg gcgtgagccg
     4741 gattccctgc ggtggaacca gatcgaccaa tggatcgtgc ggctgcggca agtactggtt
     4801 gaccgcctcg agcacgcgct cgtacgcatg tgggccggtg acgcccagca ccttcggatg
     4861 gcgttccagg attcgctgcg gattggcgcc caggcagccg gtcaccagca cgcgaccgtt
     4921 cttccgcaat gcttcgccga tggcgtcgag ggattcatcg atggccgcgt cgatgaatcc
     4981 gcaggtgttg accaccacca ggtcggcatt gtcataaccc ggtgcgatca cgtagccgcg
     5041 cgagcgcagc tgggtgatta gccgttcgga atccaccagg gccttgggac agccgaggct
     5101 cacgaagccg acggcggggg cgcgacggcg caccgaagct ttgcgttggg aagacatggg
     5161 aattgatata ttacgacgca tgcccttcga ccgcgactac taccggcgtt tctacttcaa
     5221 tccgcaaacc gccgtgacgt cgcgggccga aatgcgcgca cgcgcgcgtc tgatcgtcgg
     5281 ctgcgtcaac tatgtgggcc tgcccgtggg cagcattctg gatgccggct gcggcgtggg
     5341 gttgttgcgc gcgccgctgc tgcgggcgtt tcgccgcgcc gactacaccg gcgttgaggt
     5401 cagtcaatat ctgtgtcaac gctacggctg gcactgcagc ccggtggaga agtttcgcag
     5461 ccgtcgccgg tttgatctgg tcgtctgcta cgacgtgctg caataccttg ctgaggacgg
     5521 cgcccgctgc gcgatcgaca atctggcgcg gctgtgtcgc ggtgcgttgt acttcggcgc
     5581 gctgaccacc gaggactggc gcgagaattg cgaccagagc cgcacacacc gcgtggcggg
     5641 attgcgtctc ggcagttggt atcggcgcga gctatcgcgc agcttccgcc cccttggctg
     5701 cggcatgtgg ctcaagaagg aactaccgct caccgtctgg aacctggatg ccgttgcctg
     5761 atgatctcat ttgaaagtga agtcaggtgc gaaagcggaa acgcggcggt caacctgagt
     5821 ttgcgcggac gcgcgtgcgt ggctggccgg agcagcgata tggaggtgtt gttcagtggc
     5881 gcgacggtct cactgccgca gtcactgcac gatgtgcgcg tcctgcaact gcagaccggg
     5941 tcgcagacgc cccgcaagta ccgcatcgag agtcgggaga tgagcgtcga gttgcaggca
     6001 cggggcatgc aaatgcaccg cgccgcggct gcgcaattgt ttgacgctgt tccgcccgcc
     6061 agagttccgt ggggcattcg tgccggctgg gtgtt
//
LOCUS       DATMRM010000088         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_361441, whole genome shotgun sequence.
ACCESSION   DATMRM010000088 DATMRM010000000
VERSION     DATMRM010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361441"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VNZ06_02675"
     CDS             <1..498
                     /locus_tag="VNZ06_02675"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWW19685.1"
                     /translation="EAMGSVDELNCAIGVLVSGGLPVPAAANCLTEIQHDLFDLGGEL
                     AIPGSHLVGEQRLAWLEEQLEQFNVALPPLKEFVLPGGGTAAAACHVARAVCRRAERR
                     CWGLARAETVDSASLRYLNRLSDLLFVLARVFARAQTGQEPLWQRKRNSSADQDIGSG
                     GTSDQ"
     gene            complement(487..1047)
                     /gene="ampD"
                     /locus_tag="VNZ06_02680"
     CDS             complement(487..1047)
                     /gene="ampD"
                     /locus_tag="VNZ06_02680"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF008758.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HWW19686.1"
                     /translation="MHSALRIDPVTGLLVGVRQVLSPHFDERPAGVTPTLIVVHGISL
                     PEGEFGGPWIDRLFTGNLLPDAHPSFASVAPLRVSAHAVIRRDGAVTQYVAFAARAWH
                     AGKSVYQGREACNDFSIGIELEGADHIPYAEPQYLSLSRLIGALCEAYPSLSQSRVVG
                     HSDIAPGRKTDPGPAFDWPRLKNLLV"
     gene            1099..1836
                     /locus_tag="VNZ06_02685"
     CDS             1099..1836
                     /locus_tag="VNZ06_02685"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HWW19687.1"
                     /translation="MRQTRCYVAGPLHAGDTLSLPEGASTHLGRVLRARVNDAVTLFN
                     GQGGEYEAQILTIDRHSVRVRIGAHVAVERESPLRLTLLQSIARGEKMDLIVQKATEL
                     GVSAIVALQAERSVVRLGDEAHRKRAEHWRAIAVSACEQCGRNRIPTIEVVADLPAAL
                     ARAPSGDTRVLLEPGAQQALAEQAPRSSAMTLLIGPEGGFAPDELALAREQQFIPCRL
                     GPRVLRAETAPLAALAVIQAVAGDLQH"
     gene            1914..>2254
                     /locus_tag="VNZ06_02690"
     CDS             1914..>2254
                     /locus_tag="VNZ06_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19688.1"
                     /translation="MLPTDLSPGTIVVFRASHRKQCEERAFMLAAVGISSELAFDGLD
                     YVVRVAQDDAPRTVGHLTQYEAERRPKPPPPPPPPTLPNAWVGCVIYVMTLVAIGLLI
                     SNGFWRLDAFDR"
BASE COUNT          395 a          706 c          747 g          406 t
ORIGIN      
        1 gaggcaatgg gtagcgtcga tgagctcaac tgcgcaatcg gagtcctcgt gtcgggcgga
       61 ctgccggtac ccgcagcggc caactgtctg accgagatcc agcacgacct gtttgatctg
      121 ggcggagaac ttgcgatccc cggctcacat ctggtagggg agcaacgtct ggcatggctc
      181 gaggaacagc tcgagcagtt caatgttgcg ctgccgccgc tgaaggaatt cgtactgccg
      241 ggcggaggca ctgccgccgc cgcatgccac gttgcgcgcg ccgtgtgccg ccgcgctgaa
      301 cggcgctgtt gggggctggc ccgcgccgag accgttgact cggcatcgct gcgctatctc
      361 aatcgcctgt ccgatctgct gttcgtgctg gcgcgggtct ttgcccgcgc gcagaccgga
      421 caggaaccgc tgtggcagcg caagcgcaac tcgtccgctg atcaggatat cggttccggc
      481 gggacttcag accagtaggt ttttcagccg gggccagtcg aacgccgggc cggggtcggt
      541 cttgcgtccc ggcgcaatgt cgctgtgtcc gaccacgcgc gattgactca gactcggata
      601 agcctcgcac aacgcgccga tcaatcggct caatgatagg tactgcggct cggcgtaggg
      661 aatgtgatcg gcaccctcga gttcgatacc gattgagaaa tcgttgcacg cttcgcgtcc
      721 ctgatagacc gacttaccgg catgccaggc acgcgccgcg aacgctacgt actgtgtcac
      781 ggcaccgtcg cgccggatga ctgcgtgcgc ggagacgcgc agtggtgcga ccgaggcgaa
      841 actcgggtgc gcgtcgggga gcagattccc ggtaaacagc cgatcgatcc agggtccgcc
      901 gaactccccc tccggcaggc tgatgccatg cacgacgatc aatgtcggcg tgacaccggc
      961 aggtcgctca tcaaaatgag gcgacagcac ctggcgcacg ccaaccaaca gtccggttac
     1021 cggatcgatg cgaagagcac tgtgcatgtt gaactcgcat gatgccaaaa tgcagcgatg
     1081 ggtacagcga gcactagttt gcgccagacg cgttgttacg tcgcggggcc gttacacgcc
     1141 ggggacacgc tgtcgctgcc tgagggcgca tccacgcatc tgggcagggt actgcgagca
     1201 cgggtcaacg acgccgtcac gctcttcaac ggtcagggtg gtgagtatga agctcagatt
     1261 ctgacgatcg atcggcacag cgtgcgggta cggatcggcg cgcacgtggc ggtcgagcgc
     1321 gaatcccctc ttcggctgac cttgctgcag tcgatcgccc ggggcgaaaa aatggatctc
     1381 atcgtgcaga aggcgaccga actcggcgtg agtgcgatcg tcgcgcttca ggccgagcgc
     1441 agtgtggtgc gactgggcga cgaggcgcat cgaaagcgtg ctgagcattg gcgcgccatc
     1501 gcggtcagcg cgtgcgagca atgcggccgc aatcgtattc cgaccatcga agtggtcgca
     1561 gacctccccg ccgcacttgc gcgtgcaccc tcgggcgata cgcgtgtgct gctggagccg
     1621 ggcgcgcagc aggcgttggc agagcaggct ccgcgatcat ccgcaatgac gctgctgatc
     1681 ggaccggaag gaggcttcgc gccggatgag ctggcgctgg cgcgcgagca gcagtttatc
     1741 ccctgtcgcc tgggtccgcg ggtattgcgt gccgaaacgg caccccttgc ggcgctggcg
     1801 gtcattcagg ctgtggctgg agatctgcag cactaggacc tgccgccttg gaaatcgctc
     1861 tctgcggccg catgatcgat ggcaggtgcc catttgcgcg tgaatcagct gccatgctac
     1921 caacggacct gtcgcccggc accatcgtcg tcttccgcgc cagccatcgc aagcaatgcg
     1981 aggagcgcgc tttcatgctc gccgcggtgg gaatctcctc tgagctggca tttgacgggc
     2041 tggactacgt cgtcagggtt gcgcaggacg atgcgccgcg caccgtcggg catctgactc
     2101 agtacgaggc cgagcgacgt ccgaaaccgc cgcccccacc gccaccgccg actctgccga
     2161 atgcctgggt cggctgcgtc atctatgtca tgacgctggt ggcaatcggg ctgctgatct
     2221 ccaacggctt ctggcgcctg gatgcattcg atcg
//
LOCUS       DATMRM010000089         3105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_38141, whole genome shotgun sequence.
ACCESSION   DATMRM010000089 DATMRM010000000
VERSION     DATMRM010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38141"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VNZ06_02695"
     CDS             <1..505
                     /locus_tag="VNZ06_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW19689.1"
                     /translation="LLNVKLDAHSEVGKGSRFALTLPAGHGTVRRTPRAAAEPAPRAA
                     HAPTRVLLVEDDAAVREATRVLLKSDGYHVTAVASLAEALAHAAKDPRLDLLVTDYHL
                     RDGQTGMQVIAALREALRIPLRAILITGDTSAAVHGLRMDPHVRLASKPIKAEELLGM
                     LTALLNA"
     gene            complement(536..1186)
                     /locus_tag="VNZ06_02700"
     CDS             complement(536..1186)
                     /locus_tag="VNZ06_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWW19690.1"
                     /translation="MNQGLPIVFIVDDDEAVRNSLRLLVKSVGLSATTLVSAQEFLAS
                     YDPLQPGCLVLDVRMPGMSGLELQQQLNLRGAVIPVIFITGHGDVPMAVAAMQQGAFD
                     FLQKPFRDQDLMDRIQRALEKDGANRAELGERSRIREQYETLTPREREVLALVTSGKA
                     NKVMAADLGLSQRTIEIHRARVMEKMGASSLAHLVRMVLDLEDSDGQGATRADSAP"
     gene            complement(1183..2310)
                     /locus_tag="VNZ06_02705"
     CDS             complement(1183..2310)
                     /locus_tag="VNZ06_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWW19691.1"
                     /translation="MSAYRPLEQELRSLLDAAVEGIVVIDHLGCIQAFNRSAERLFGY
                     QAQEVLDRNVNVLMNEPDRIAHDGHLARYAATRVARIIGNGREVSARRKDGSVFPAFL
                     LVRAVADSEPPRFVGFVHDMTPRRQGEEHARRLQERLWHVSRLATVGEMASGIAHELN
                     QPLAAIANYAHACDRLLGRPDADLEEVRGALKEIAAQAVRAGDTIRRLRSLAHPLVDR
                     REPTEINVLITELTALVRSDAQAHDVQYRLELSDGLPRLEVHRTQIQHVILNLVRNAV
                     ESLAETPAKPREIVVRTSRTRDGEVEVSVCDSGPGVSPSVAPHLFDPFWTSKPAGAGL
                     GLAISRTIIAEHGGTLDYRPNVPVGACFALRLPVVNRGEQT"
     gene            complement(2431..>3105)
                     /locus_tag="VNZ06_02710"
     CDS             complement(2431..>3105)
                     /locus_tag="VNZ06_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWW19692.1"
                     /translation="GLIAPGIFLPLLESAGLMGATGAWVLRQAASDCRAWRCKGLPPI
                     RVAINISPAELRQRNIAGEVLEAIGDLAGDPAWGIDLEITEGVLSGDSSSCVHALRLL
                     RAAGVRIAIDDFGTGFSSLSRLSELPIDTLKIDRVFTSRLPTDRRSCTLVSTIIGLAH
                     AFDMTTVAEGVETQAQLDYLVREGCDESQGYLHSRPLPRSDLESWLIGVGSAATDGSD
                     LRSARA"
BASE COUNT          552 a         1020 c         1000 g          533 t
ORIGIN      
        1 gctgctcaat gtcaagctgg atgcgcattc cgaggtcggc aaaggctcgc gcttcgcgct
       61 caccctgccg gcgggccacg gtaccgtccg ccggacaccc cgggcagcag cggagccagc
      121 gccgcgcgcc gcgcacgcac cgacacgcgt tttactggtg gaggacgatg ccgccgtgcg
      181 agaggcgacc cgtgtgctgt tgaagagtga cggctatcac gtcacggccg ttgcctccct
      241 ggccgaagcg ctggcgcacg cggccaaaga cccgcgtttg gatctgctgg tgaccgacta
      301 tcatctgcgc gacggacaga ccggcatgca ggtcattgcg gcgctgcgcg aggccctgcg
      361 gattcccttg agagcgatcc tgatcaccgg cgacacctct gcggccgtac acggcctgcg
      421 catggacccc catgtgcggc tcgcgagcaa gccgatcaag gccgaggagt tgctggggat
      481 gctcactgcc ctgctcaatg cctgagcggc agcccagcca gaccggttga gccgctcagg
      541 gggcagagtc ggccctggta gcgccttgtc cgtcgctgtc ctcgaggtcc agtaccatcc
      601 gcaccaggtg agcgagcgag gaggcgccca tcttctccat tacgcgggca cggtgaatct
      661 cgatggtgcg ctggctcagg ccgagatcgg cggccatgac cttgttggcc tttccgctcg
      721 tcaccagcgc cagcacctca cgctcgcgtg gcgtcagcgt ctcgtactgc tcgcgaatgc
      781 ggctgcgctc gccgagctcg gcgcgatttg ccccatcctt ctccaacgcc cgctgaatgc
      841 ggtccatcag gtcctgatcc cgaaagggct tttgcaggaa atcgaaggcc ccctgctgca
      901 tggccgctac ggccatggga acatcaccat gcccggtgat gaaaattacc ggaatgacgg
      961 caccgcgcag gttcaattgc tgttgcagct cgaggccgct cataccgggc atgcgcacgt
     1021 caaggaccag gcacccgggc tgcagcgggt cgtagctcgc caggaactcc tgagcggaga
     1081 ccaatgtcgt ggcactcagg ccaaccgact tgaccagcag ccgcagcgag ttgcgcacgg
     1141 cttcgtcgtc atcgactatg aaaacaatcg gtaatccctg attcatgtct gctccccgcg
     1201 attcaccacc ggcaggcgca aggcgaagca ggcaccgacc ggaacgttcg gccgataatc
     1261 cagtgtaccg ccgtgctctg cgatgatggt acggctgatc gcaaggccga ggcccgcgcc
     1321 ggcgggtttg gacgtccaga atggatcgaa caggtgcggg gcgacgctcg gggagacccc
     1381 ggggccgctg tcacacaccg atacctccac ctctccgtcg cgggtccggg aagtgcgcac
     1441 gacgatctca cgcggttttg caggagtctc ggcaagtgat tcgacggcgt tgcgcaccag
     1501 attgagtatg acgtgctgga tctgggttcg gtgcacctcc aggcgcggca gcccgtcgct
     1561 gagctcgagg cggtattgca catcgtgggc ctgcgcatcc gagcgcacca gggcggtgag
     1621 ttcagtgatc agcacgttga tctcggtcgg ttcgcgccga tcgacgagcg ggtgcgccag
     1681 gctgcgcaac cgacggatgg tgtccccggc acgcaccgcc tgtgccgcga tttctttcag
     1741 ggcgccccgt acctcttcga gatccgcatc cggccggccc aacagccggt cgcaggcatg
     1801 agcatagttc gcaatcgccg ccagcggctg gttgagttcg tgggcgatgc cggacgccat
     1861 ctcaccgacg gtggcaaggc gagatacgtg ccacaaacgc tcctgcagcc gacgcgcgtg
     1921 ttcctcgccc tggcgtcttg gcgtcatgtc atggacgaaa ccgacaaagc gtggcggctc
     1981 gctgtccgcg accgcgcgga ccagcaggaa tgccggaaag acgctgccgt cttttcgccg
     2041 tgcgctcact tcacgaccgt ttccgataat gcgcgcaacg cgagtcgccg cgtatcgtgc
     2101 caggtggccg tcgtgcgcaa tacgatccgg ctcgttcatc agcacgttga cattgcgatc
     2161 cagcacttcc tgtgcctggt agccgaacag ccgctcggct gagcgattga aggcctgaat
     2221 gcagccgaga tggtcgatca ccacgatccc ttcgacggcg gcatccagca gcgaacgcag
     2281 ctcttgttca agcggacgat aggcggacat gcgggaaatt atgcatgccg ggctcaggcc
     2341 gcgtctctag gtatgttccg caaccacaga cgcccctgga ggcgggagat cggccggggg
     2401 gcgggggatg agtcgcgcgc gcatccaggc tcaggcacgc gcgcttctga gatcgctccc
     2461 gtccgttgcg gcgctgccga caccgatgag ccagctctcc aaatccgatc ttggcagcgg
     2521 tcggctgtgg aggtagccct gtgattcatc gcagccttcg cgcaccaggt aatccaactg
     2581 cgcctgagtc tcgacgcctt ccgcgacggt cgtcatgtcg aacgcatgcg ccaaaccgat
     2641 gatcgtggag accagcgtgc agctcctgcg gtccgtcggc agacggctgg tgaacacccg
     2701 gtcgatcttg agagtgtcaa tcggcagctc cgacaggcgg ctgagcgagg agaaacccgt
     2761 accgaagtca tcaattgcta tacgaacgcc cgcggcgcgc aacagcctga gcgcgtggac
     2821 gcacgatgag gagtcgccgg aaagcacacc ctcggtgatc tcgaggtcga tcccccaggc
     2881 cggatcgcca gccagatcgc cgatggcctc gagaacctcg ccggcaatgt tgcgttgtcg
     2941 cagctccgcg ggagaaatat tgatggccac tctgatgggc ggcaggccct tgcagcgcca
     3001 ggcgcgacag tcggacgccg cttgcctgag cacccaggcg ccggtcgccc ccatcaggcc
     3061 ggcggattcg agcagcggca ggaagattcc tggcgcgatc agtcc
//
LOCUS       DATMRM010000090         6224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1894567, whole genome shotgun sequence.
ACCESSION   DATMRM010000090 DATMRM010000000
VERSION     DATMRM010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1894567"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /gene="trkA"
                     /locus_tag="VNZ06_02715"
     CDS             complement(<1..1041)
                     /gene="trkA"
                     /locus_tag="VNZ06_02715"
                     /inference="COORDINATES: protein motif:HMM:NF007032.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HWW19693.1"
                     /translation="MKILILGAGQVGRTAAYHLSREPANEVTVVDTNDDVLRDLQDRL
                     DIRAVAGNGSYPAVLEAAGIADTDILVALTSSDEVNIVACGIAQALYRTPTKIARVRA
                     PEYTTREQLFAEGALAVDVWISPEQLVTEYIAHLLSYPGALQVVDFADGRVQLVGVRT
                     RGDGLLVGRALKTLREHLPDTEMRVAAIYRNDRLIEVTGETVIQENDEIFFVAASDDL
                     RRVMAELRRSEDPVRRLVIAGGGNIGLRLARTLEKSNQVKLIEREPARARRISEILEN
                     TIVLNGDAADEELLIEENIDSADVFAALTNSEEANVLSAMLAKRLGAKRVMALINRPS
                     YGELMENHSIDIV"
     gene            complement(1063..4320)
                     /locus_tag="VNZ06_02720"
     CDS             complement(1063..4320)
                     /locus_tag="VNZ06_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWW19694.1"
                     /translation="MDLSPILNPLNDAQREAVAAPLGPVLVLAGAGSGKTRVLIHRIA
                     WLIQTEGVSPHGILAVTFTNKAAAEMRGRVEKLLGSPGGALWLGTFHGLAHRLLRLHW
                     REARLPQGFQILDAEDQARLIKKLLKAQGLDETRWVPRDVQYFINKHKDEGRRPQNLH
                     AGHDPTEQQMIKLYALYEETCARSGVIDFAELLLRAFELWRDDSELLQHYRRRFRHVL
                     VDEFQDTNSIQYAWIRLLVGSEGAPFVVGDDDQCLAAGTLVSMGDGTKRPIESIAPGE
                     LVMSSYGGGDLRPARVTERFVHRRRGRLIRLHLRSGALIKSTPEHTHFAGYVLGETPQ
                     TCFLYLMHKEGIGYRLGTSQLYPKGQAKPMLGFKQRAIQEHADAVWIIRTHQNENEAR
                     FDEMTTSLRFGLPTLPSTPQEGKALNPLVHDPCSIARVFKSIDTTLAAERLLEEVALD
                     IDRPHHLPRSRNSSRRNIVITLCADRRGSSPMHTICMVGHSTGDRATLESLGLRVRAA
                     RRHRDSWRYETARDDFGELMAIARSIRDRLDGRYVLQGRMLETSLPFITAASIRPGMV
                     MATDQGKFEVIEHISHEDFDAEVFDINVERTHNFVAQGVVTHNSIYRWRGAQVENLHK
                     FRQDFPHAQLCRLEQNYRSTSSILDAANALIANNTGRLGKTLWTSGAKGEPVKLYAAY
                     NERDEADFVVTRILEWTQRGGVRRECAILYRSNAQSRAFEEALIQARVPYRVYGGLRF
                     FERAEIKDALAYLRLITNRDDDASFERVVNLPPRGIGAKSVDAIREAARGAGSSLWRA
                     AAASVSSGEVGRAATALLGFMQLIEKLAHGIEALPLYEQVDQAITGSALIEHYQKDKA
                     DRGEARVENLLELVSAARGYEPEGDMPPLQSFLAHAVLESGEAQGDAWEDCVQMMTLH
                     TAKGLEFPLVFLCGLEDGLFPHQRSVNDLDGLEEERRLCYVGMTRAMKQLYFTYAEQR
                     RMHGVDSYNAPSRFIQEIPPAMIAEVRPRIRVSQSHLPSSIAVPKRRRGWDTRSLTES
                     IGGGMRLGTRVRHGKFGEGVVLTVEGQGPHARVQVNFEQQGSKWLMLQYANLEVM"
     gene            complement(4341..5138)
                     /locus_tag="VNZ06_02725"
     CDS             complement(4341..5138)
                     /locus_tag="VNZ06_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HWW19695.1"
                     /translation="MLIERVWAANAGRNFHYLIACGATGEALAVDPLDAARCLRRARE
                     LGWTIRQIFNTHEHVDHTGGNADMVAATGAAVLAHRDAAARIEGVTHGLNGGDSVRIG
                     RSVELRCLDTPGHTRAHLCLYGSPQDNDVPALFSGDTLFNAGVGNCIHGGDPRLLYDS
                     FSSTLSRLPDATRVFPGHEYLVRNLGFTLDREPSNAAAREMAQRCSGLAAENMPVTNL
                     GEERQFNAFLRLESPEIIEGLRSRTEWKNTPGPREVFLALRELRNRW"
     gene            5184..6032
                     /locus_tag="VNZ06_02730"
     CDS             5184..6032
                     /locus_tag="VNZ06_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWW19696.1"
                     /translation="MQLFVVGVVLFAALIHASWNALLKTGSDTVLTTALMTTAAACIA
                     AVALPFLPVPAAPSWPYMAGSVALQFIYYTLLVRAYATGDMSHTYPIMRGGAPFIVAA
                     LSASLIGEPLTAARWLGVGLICGGVLGLAIHRTARPTPHHAATAYALANAGAIAAYTL
                     VDGMGVRKSQAPASYTLWLFLLIGAVRLGWTFLLRRRELLGYLRRYWPQGVAGGVGTL
                     SAYGLALWAMTVAPVALVAALRETSILFATVISALVLKERVTPMRVVSAVLIAAGAAT
                     LAAQRR"
     gene            complement(6012..>6224)
                     /locus_tag="VNZ06_02735"
     CDS             complement(6012..>6224)
                     /locus_tag="VNZ06_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19697.1"
                     /translation="YMTGMSGLLYLGFAVVLNARFLWQAIALKVYGRDELPMRMFRFS
                     ISYLMWLFLGLLADHYLQGLLPALRG"
BASE COUNT         1096 a         2011 c         1927 g         1190 t
ORIGIN      
        1 cacgatgtcg atactgtgat tttccatcag ctcgccgtag gacgggcggt tgatgagcgc
       61 catcacccgc ttggcgccca ggcgcttggc cagcatggcc gagagcacat tggcttcctc
      121 cgagttcgtc aacgccgcga acacgtcggc gctgtcgatg ttctcctcga tcagcagttc
      181 ctcgtcagcg gcatcgccgt tgagcacgat ggtgttctcc agtatctcgg agatgcgccg
      241 tgcgcgtgcc ggttcgcgct cgatcaattt gacctgattg ctcttttcca gcgtccgcgc
      301 caggcgcagt ccaatgttgc caccgccggc gatcaccagg cgtcgcaccg gatcctcact
      361 gcggcgcagt tccgccatca cgcggcgcag atcgtccgag gcggctacga agaagatctc
      421 atcgttttcc tggatcacgg tctcgccggt gacctcgatc agacggtcgt tgcgatagat
      481 ggcggcaacg cgcatctcgg tgtcgggcag atgctcgcgc agcgttttca acgcgcgtcc
      541 gaccagcaaa ccatcgccgc gggtacgtac ccccacaagc tggacgcgtc cgtcggcgaa
      601 gtccactacc tgtagggcgc ccggatacga cagcaggtga gcaatgtact cggtgacgag
      661 ctgctcgggg ctgatccaca cgtcgaccgc gagcgcacct tcggcgaaca actgctcgcg
      721 cgtggtgtac tccggtgccc tcacacgggc gattttggtg ggcgtgcgat agagagcctg
      781 cgcaatgccg caggcaacga tgttgacctc atcgctgctg gtgagcgcta ccaggatgtc
      841 ggtgtcggcg atccctgcgg cttccagcac cgcgggatag gaaccgttgc cggccacggc
      901 acgaatgtcg agccggtcct gcagatcgcg cagcacgtcg tcattggtgt cgacaaccgt
      961 gacttcgttg gccggctcgc gcgagaggtg gtaggccgcg gtgcgtccga cttggccggc
     1021 gcccaggatc aggatcttca tcgcgcttgc gctccgtcgc tgctacatca cttcgagatt
     1081 cgcgtattgc agcatcagcc acttggagcc ctgctgctcg aaattgacct gtacgcgtgc
     1141 gtgcggcccc tgaccttcca cggtcagaac cacgccttcc ccaaacttgc cgtgtcgcac
     1201 ccgtgtcccc aaccgcatgc cgccgccgat cgactctgtg agcgatctgg tatcccagcc
     1261 gcgccgacgc ttcggcacag cgatggaaga gggcaggtgc gactgtgaaa cccggatgcg
     1321 aggcctgact tccgcaatca ttgcgggcgg aatttcctgg ataaatctcg aaggtgcatt
     1381 gtagctgtcc acgccatgca tccggcgttg ttcagcataa gtgaaataga gctgcttcat
     1441 cgcgcgcgtc atgccgacgt agcacagccg gcgctcctcc tcgaggccgt ccagatcgtt
     1501 gaccgagcgc tggtgtggga acagcccatc ttccaggcca cacaggaaaa ccagcggaaa
     1561 ctccaggccc ttcgcggtgt gcaacgtcat catctgcaca caatcttccc acgcatcgcc
     1621 ctgggcctcc cccgactcca ggaccgcatg cgcgaggaag ctctgtaggg gaggcatgtc
     1681 gccttcgggt tcgtaaccac gcgcggcgct gaccagttcc agcagattct cgacacgggc
     1741 ctccccgcgg tcggccttgt ccttctgata gtgctcgatc agagcgctgc cggtgatggc
     1801 ctgatcgacc tgctcgtaca acggcaaggc ctcaatgccg tgcgccagct tctcgatcag
     1861 ttgcatgaac ccgagcaacg ccgtcgccgc gcgcccgacc tcgccggagg agaccgaggc
     1921 ggccgcagca cgccagagcg agctaccggc gccgcgggcc gcctctcgaa tcgcatccac
     1981 gcttttggca ccgattccgc gtggcggcag attcaccacc cgttcgaagg acgcatcatc
     2041 gtcacgattg gtgatcaagc gcaggtacgc gagcgcgtct ttgatctcgg cgcgctcgaa
     2101 gaaccgcagt ccgccgtaga cgcgataggg cacgcgcgcc tggatgagcg cttcttcgaa
     2161 ggctcgtgac tgcgcgttcg accgatagag aatggcgcat tcgcgccgca cgccgccgcg
     2221 ctgggtccac tcgaggatgc gcgtcacgac gaagtccgcc tcatcgcgct cgttgtacgc
     2281 cgcgtacagc ttgaccggct cgcctttggc gccactggtc cacagggttt tacccagtcg
     2341 tccagtgttg ttcgcgatca gcgcgttggc cgcatcgaga atcgaggaag tcgagcgata
     2401 gttctgctcc agccggcaca actgggcgtg cgggaaatcc tggcggaact tgtgcagatt
     2461 ctcgacttgg gctccgcgcc agcggtagat cgaattgtga gtcaccacac cttgcgcgac
     2521 gaagttgtgc gtgcgctcga cattgatgtc aaagacctcg gcgtcgaagt cctcgtggct
     2581 gatgtgttcg atcacctcga atttcccctg atccgtcgcc atcaccattc cggggcggat
     2641 gctcgcggca gtgatgaatg gcagcgacgt ctccagcatg cggccttgca ggacgtatcg
     2701 gccatccagt cggtcgcgga tgctgcgtgc gattgccatc agctcgccga agtcgtcccg
     2761 agctgtctca taacgccagc tgtcacggtg ccgtcttgca gcccgaacac gcaagcccag
     2821 gctctccagg gttgcccgat ccccggtgct atggccgacc atacagatag tgtgcatcgg
     2881 tgaactgcca cgtcggtcgg cgcacagcgt gatgacgatg ttccgcctgc tcgaattgcg
     2941 acttcgtggc aaatggtgag gtcggtcgat atcgagagcc acctcttcga gcagtcgctc
     3001 ggcggcgagt gtggtgtcga tcgatttgaa gacgcgcgcg atagagcacg gatcgtgtac
     3061 cagggggttg agcgccttac cttcctgggg tgtggacggc agcgtcggca agccaaagcg
     3121 cagcgacgtc gtcatctcat cgaagcgcgc ctcgttctca ttctggtgcg tccgaatgat
     3181 ccagaccgca tcggcgtgct cctgaatcgc gcgttgcttg aatccaagca tcggcttcgc
     3241 ctgacccttc gggtagagct gcgaggtgcc gaggcggtaa ccgatacctt ccttatgcat
     3301 cagatacaga aagcaggtct gaggcgtctc acctaatacg tagcccgcga aatgcgtatg
     3361 ctccggcgtg ctcttgatca gcgcgccgga gcgcaagtgc aagcggataa gcctcccccg
     3421 ccgccgatgg acaaagcgct ccgtgacgcg ggccggccgc agatcgccgc cgccatagct
     3481 ggacatcacc agctcgcccg gcgcaatcga ctcgatgggc cgtttcgttc cgtctcccat
     3541 gctcaccagc gtgccggcgg cgagacattg atcgtcatca cccacgacaa acggcgcccc
     3601 ttccgatccc accagcagcc ttatccatgc atactggatc gaattggtgt cctggaactc
     3661 atcgaccagg acgtgacgaa accgtcggcg atagtgctgc agcaactcgc tgtcgtcgcg
     3721 ccacagttcg aatgcgcgca gcagcagctc ggcgaaatcg atgacacctg agcgtgcaca
     3781 agtttcctcg tacagcgcat acagtttgat catctgctgc tcggtcggat cgtggccggc
     3841 gtgcaggttc tgcgggcggc ggccctcgtc cttgtgcttg ttgatgaagt actgtacgtc
     3901 gcgcggtacc cagcgcgttt cgtccagacc ctgggctttc agcagtttct tgatgagccg
     3961 cgcctgatcc tcagcgtcca ggatctggaa gccctgcggc agacgcgcct cgcgccaatg
     4021 caagcgcagc agacgatggg cgaggccatg aaaggtcccc agccacagcg cgccgccagg
     4081 ggagcccagc aacttctcaa cgcgaccgcg catctcggcg gcggccttgt tagtgaatgt
     4141 gacggccaga attccgtggg gtgaaacacc ctcggtctga atgagccagg cgatgcggtg
     4201 aatcaagacg cgcgttttgc cgctacccgc gccggccagt accagtaccg gtccgagcgg
     4261 ggcggcgacg gcctcgcgct gggcgtcgtt caacggattc agaatgggcg ataggtccat
     4321 gagggccgcg aattctaccc ttaccagcga ttgcgcagtt ccctcagggc gagaaatacc
     4381 tcgcgcgggc cgggcgtgtt tttccactcg gtccgggaac gcagcccctc gatgatctcg
     4441 ggactctcga gccgcagaaa agcgttgaat tgacgctctt cgcccagatt ggtgaccggc
     4501 atgttctcgg cggcgagtcc actgcagcgc tgcgccatct cgcgggctgc ggcattggat
     4561 ggttcccgat cgagcgtgaa gccaagattg cgcaccaggt actcatgacc cggaaatacc
     4621 cgcgtggcgt cgggcagccg cgacagcgtc gagctgaaac tgtcgtatag cagccgcgga
     4681 tcgccaccgt gaatacaatt gccgaccccc gcattgaaca gcgtatcgcc ggaaaacagc
     4741 gccggcacgt cgttgtcctg cggcgagcca tacaggcaca gatgcgcacg cgtatgaccg
     4801 ggtgtgtcca ggcagcgcag ttcgaccgag cgcccgatgc gaacgctgtc accaccgttg
     4861 agaccgtggg tcaccccctc gatgcgagcc gccgcgtccc ggtgcgccag caccgcggca
     4921 ccggtggcgg ccaccatgtc ggcattgccg ccggtgtggt ccacgtgctc atgggtgttg
     4981 aagatctggc ggatcgtcca accgagctcg cgtgcgcgac gcaggcagcg cgccgcatcg
     5041 agcggatcga ccgccagcgc ctcaccggtt gcgccacagg cgatcagata atggaagttg
     5101 cggccggcat tggccgccca gacgcgctct atcagcatga tgcctaccta cgtgtctcgt
     5161 ggcgatcagg caatattaag cgcatgcagc tctttgtcgt aggcgtcgtg ctgtttgcag
     5221 cgctgattca tgcgtcctgg aacgcgctgc tcaagaccgg ctcggacacg gtcctgacga
     5281 ccgcactgat gaccacggct gcagcctgca tcgcggctgt ggcgctgccc tttctgccgg
     5341 tacccgccgc cccgagctgg ccctacatgg caggctcagt ggcgctgcaa ttcatctact
     5401 acacgctgct ggtgcgcgcc tatgcgaccg gcgacatgag tcatacctac ccgatcatgc
     5461 gcggcggcgc accgttcatc gtcgcagcgc tcagtgcatc gctgatcggc gagccgctga
     5521 cggcagcacg ctggctgggt gtgggcctga tctgcggcgg cgtgttgggt cttgcgattc
     5581 accgcactgc acgcccgaca ccgcaccacg cggcgaccgc ctatgctttg gccaacgccg
     5641 gcgcgatcgc cgcctacacg ctggtcgatg gcatgggggt gcgaaagtcg caggcgccgg
     5701 cgtcgtacac gctgtggctg tttcttctga tcggcgccgt gcgcctgggc tggacattcc
     5761 tgctgcggcg gcgtgagctg ctcgggtatc tgcgtcgtta ctggccgcaa ggcgtggccg
     5821 gcggtgtcgg taccctcagt gcgtatggtc tggcgctgtg ggccatgaca gtggcccccg
     5881 tcgcactggt cgccgcgttg cgcgagacgt cgatcctgtt tgcgacagtg atttctgcgc
     5941 tggtactcaa ggaacgcgtg acgccgatgc gtgtggtttc cgcggttctg atcgcagccg
     6001 gggctgcgac gctagccgcg cagcgccggt agcagcccct gcagatagtg atcggccagc
     6061 aggccgagga acaaccacat cagatagctg atcgaaaagc ggaacatgcg catcggcaat
     6121 tcgtcgcgcc catatacctt cagagcgatc gcttgccaca ggaaccgagc gttgagtacc
     6181 acagcgaagc ccaggtagag cagtccgctc atgccggtca tgta
//
LOCUS       DATMRM010000091         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3136133, whole genome shotgun sequence.
ACCESSION   DATMRM010000091 DATMRM010000000
VERSION     DATMRM010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3136133"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /gene="dusA"
                     /locus_tag="VNZ06_02740"
     CDS             <1..954
                     /gene="dusA"
                     /locus_tag="VNZ06_02740"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: protein motif:HMM:NF008774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HWW19698.1"
                     /translation="VLLYTEMITASAIIYGNRQRLLQFDPEEQPLALQLGGSDPAQLS
                     IAARAGQDLGFCEINLNCGCPSDRVHTGAFGACLMMRPELVAECVEVMSASVSLPVTV
                     KMRIGVVSGPQARLQVGRFDENDYQQLHSFTEKVAAAGCRRFIVHARKAVLGGLSPRE
                     NREVPPLRFDVVQRLRVDFPALSIGVNGGLRTVCQCRQALAWADGVMLGREAYHRPYL
                     LAQLHRELFDGDWRCPEPAQLLERMAIYAQRQLCAGERLSSITRHMLGLCTGQPGARR
                     FRQRLSEGARQIQPGPQASADAARLLRDVAGLCVAESMARV"
     gene            1027..1932
                     /locus_tag="VNZ06_02745"
     CDS             1027..1932
                     /locus_tag="VNZ06_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWW19699.1"
                     /translation="MGKDIELVILFADVVGSTRLFEVMGDLKARDMVTTCVDLMRSAT
                     EQHNGTVIKTIGDEVMATFPTADDALNAAGQMQKEIATHSDLKVEGQPVAIRIGCHFG
                     PVVLESKDVFGASVHTANRMTSQAKAGQIITTAATVERLSPEWRASVRQIDVATIRGQ
                     GGEVALYEVLWQTEDVTSMLPAIALTGRDNRKPQRLRLSVQDRELILDENRPQIAIGR
                     ADDNDVVIRGNLISRLHARIEFSRNRFTLTDQSTNGTFVQISGEEEAFVRRDSMPIKG
                     QGLIGLGRVPDRDSPLTIHFVCEDS"
     gene            complement(1929..>2426)
                     /locus_tag="VNZ06_02750"
     CDS             complement(1929..>2426)
                     /locus_tag="VNZ06_02750"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF013019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HWW19700.1"
                     /translation="SVASETTAAQSGAVGVVRRDPMAMKPFAGYNYGDYWTHWLNLGA
                     RLKNPPRIFHVNWFRQNAAGKFLWPGYSDNLRVLEWVLDRCAGRGGAVDTPIGFIPRP
                     GDIDTQGMTIDQATLTELTAIPNEAWVKEMSDFRKYLLQYGSRLPAKLVAEVDEVERR
                     LKARS"
BASE COUNT          441 a          728 c          796 g          461 t
ORIGIN      
        1 gttctgctgt ataccgaaat gatcaccgcg tcggcgatca tttatggcaa tcgccagcgg
       61 ctgctacagt tcgatccgga agagcagccc ctggcgctgc agctcggagg cagtgatccg
      121 gcacaactgt cgatcgcggc ccgggcaggg caggaccttg ggttttgcga gatcaatctg
      181 aactgcggct gtcccagcga ccgcgtgcat acgggcgctt tcggggcctg tctgatgatg
      241 cgaccggagc tggttgccga gtgcgtggag gtcatgtctg cgtccgtctc gctaccggtc
      301 accgtcaaga tgcgcattgg cgttgtcagc ggaccgcagg cgcgcttgca agtgggacgg
      361 ttcgatgaaa acgactatca acagctgcac agcttcacgg aaaaagtcgc cgcggccggc
      421 tgtcgccgtt tcatcgttca tgcccgcaaa gccgtgctcg gtgggctctc gccgcgcgag
      481 aatcgcgaag tgccgccgct gcggttcgat gtcgtccagc gactgagggt ggactttccc
      541 gcgctttcga tcggtgtgaa cgggggacta cgcactgtct gtcaatgccg acaggcgtta
      601 gcgtgggcgg atggggttat gctgggacga gaggcctatc atcgacctta cctgctggcg
      661 cagttgcatc gagagctctt cgacggggat tggcgctgtc cggagccggc gcaactgctt
      721 gagcggatgg cgatctatgc acagcggcaa ctgtgtgccg gggagcggtt gtcctcgatt
      781 acgcggcata tgctgggcct gtgcaccggc cagccggggg cgcggcgttt cagacagcgt
      841 ctgtccgagg gcgcgaggca gattcagccg ggcccgcagg cgtccgccga tgcggccagg
      901 ctactgcgtg acgtggcggg cctctgcgtg gcggaaagca tggctcgcgt ataatctcgc
      961 gctggcgccc gcttaatcaa ctattctccg ttgcaagaca actcattgca gtaacagtcg
     1021 gaatacatgg gcaaagacat tgagctcgtg atcctgtttg ccgacgtcgt cggctctacc
     1081 cgcttgttcg aagtgatggg ggatctgaaa gcgcgcgaca tggtaacgac ttgcgtggac
     1141 ctgatgcgca gcgcgaccga gcaacacaat ggcacggtca tcaagacgat cggcgatgag
     1201 gtgatggcca cgtttccgac cgccgatgac gccctcaacg cggcgggtca gatgcaaaag
     1261 gagatcgcga cccatagcga cctgaaggtg gagggccagc ccgtggcgat acggatcggc
     1321 tgccacttcg gtcccgtggt actcgaatcc aaggatgtgt ttggcgccag cgtacacacc
     1381 gccaaccgca tgaccagcca ggccaaggca ggccagatca tcaccaccgc cgccacggtg
     1441 gagcgactct cacccgaatg gcgcgcttcg gtgcgacaga tcgatgtcgc gaccatccgc
     1501 gggcagggcg gggaggtggc gctgtatgag gtgctgtggc agaccgagga tgtcaccagc
     1561 atgctgccgg ccattgcgct caccggtcgc gacaaccgca agccgcaacg gctgcgtctg
     1621 tcagtgcagg atcgcgagct gatcctggat gagaaccggc cgcagatcgc gattggtcgt
     1681 gccgatgaca acgacgtcgt gatacgtggc aacctgatct cgcgcctgca cgcgcgcatc
     1741 gaattcagcc gtaaccgttt cacgctgacg gatcagagca cgaacggcac gttcgtgcag
     1801 atctcgggtg aagaggaagc cttcgtccgc cgtgacagca tgccgatcaa gggtcagggg
     1861 ttgatcgggc tgggtcgcgt gcccgaccgc gactcgcccc tgacgattca cttcgtctgc
     1921 gaggacagct agctcctggc tttcagtcga cgctcgacct catcgacttc cgccaccagc
     1981 ttcgctggca ggcgtgagcc gtactgcaga agatacttgc ggaagtcgct catctctttg
     2041 acccaggctt cgttcgggat agcggtcagt tcagtgagcg tcgcctgatc gatcgtcatc
     2101 ccctgggtgt cgatgtcgcc cggccgggga atgaatccga tcggcgtatc gacggcgccg
     2161 ccgcggcccg cgcagcgatc cagcacccat tccagcaccc gcaggttgtc gctgtagccc
     2221 ggccacagga atttgccggc cgcgttttgg cggaaccagt tgacgtggaa gatgcgcggc
     2281 ggattcttca gcctggcacc gaggttcagc cagtgcgtcc agtagtcgcc gtagttgtag
     2341 ccggcaaatg gcttcattgc catggggtca cggcgcacca cacctaccgc accgctctgg
     2401 gcagcggtcg tttcggaggc gaccga
//
LOCUS       DATMRM010000092        20572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3760142, whole genome shotgun sequence.
ACCESSION   DATMRM010000092 DATMRM010000000
VERSION     DATMRM010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 20572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20572
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3760142"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VNZ06_02755"
     CDS             complement(<1..1150)
                     /locus_tag="VNZ06_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HWW19701.1"
                     /translation="MSPRFAAAPFLPTSRAQMQALGWDQCDVVVVSGDAYVDHPSFGM
                     AIIGRVLEAQGLKVGVIAQPDWHSAEGLRVLGKPRLFFGITAGNMDSMVNRYTADRRV
                     RSDDAYTPGGEGGRRPDRSVIVYAQRAREAWRDVPIVIGGIEASLRRIAHFDYWSEKV
                     RRSILLDAKADLLVYGNGERQIIEIARRLDAGERIEALQDIRGTAFIQRSMCADWIEI
                     DSTHLDAPGALMPPVDPYAAEPRAASAPQACGDGAQVVRFVRRVRNADRERSVIRLPA
                     FEQVSDDPVMYAHASRILHLESNPGNARALVQAHGSQQVWLNPPPIPLTSAEMDWIYD
                     LPFQRRPHPAYGEALIPAYKMIRFSVAIQRGCFGGCTFCSITEHEGRII"
     gene            1265..6745
                     /gene="uvrA"
                     /locus_tag="VNZ06_02760"
     CDS             1265..6745
                     /gene="uvrA"
                     /locus_tag="VNZ06_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWW19702.1"
                     /translation="MSPRAHDAIVVRGARQNNLRDLDLDLPLNELIVVTGVSGSGKSS
                     LVFDTLYAEGQRRYVETFSPYARQFLDRMDKPQVDRIERIPPAIAIDQVNPVRTSRST
                     VGTMTELNDHLKLLFARAARLDCRQCHKPVRRDNAQSIADALPGLLAEFERPLLLICF
                     PVPLPAQLKMAELKAELEKLGYVRIHQEQPKLLTMIQDRLRLQGEDRSRLVEALEAAL
                     RSGHGRVSIHALDTKGAERASLRFSNDLHCADCDLHYQDATQGLFSFNSPLGACDTCR
                     GFGRMIGIDYGLVVPDTGKSLAGGAIRPWQTPSYKECQDDLLRFGKKRGIALDTPWRE
                     LSDEQRRWVIEGEGAWEKKVWYGARRYFAWLETKAYKMHIRVLLSKYRSYTLCHTCQG
                     ARLKPDALLWRLGDSHGLAIHEVMQLPIERCHEFFLHLRLPKPLDQAADLLLGELRTR
                     LRYLCDVGLGYLTLDRQSRTLSGGEVQRINLTTALGTSLVNTLFVLDEPSIGLHPRDM
                     QRVIGVMKRLRDAGNTLLVVEHDPQIMIEADRILDLGPGAGEHGGSIVCYGTPREVMV
                     HPSSATGAWLAGRRRLPPLHATADSPRRTGNAATLRLTGAAEHNLKQLDVDIPLHRLV
                     CLTGVSGSGKSTLVQDVLFPALQKAHGKPSESPGAFRTLSGAELIDAAVLIDQTPIGR
                     TTRSNPASYVGAFDEIRARFAQEPESRLRRYTAGTFSFNSGNGRCPACGGNGFEHVEM
                     QFLSDVYLRCGECNGSRYRSETLQVRIAGANNRRAHIAQVLDMTATEALTFFSDVTAI
                     CARLQPLVDVGLDYVRLGQPVPTLSGGEAQRLKLAGHLAQAALGNGRNSREKDKPSLG
                     KLLIFDEPTTGLHFEDIARLVSAFGLLLQAGHSLLVIEHNLDVIRAADWVIDLGPEGG
                     DRGGEIIAAATPGDLMRIARSHTGRALSQYAQAQYTVAEPSGSYEAATVTPAHGSVTV
                     RGAREHNLKNINVEIPHERFTVITGVSGSGKSTLAFDLLFNEGQRRYLESLNAYARQF
                     VQPAARPDMDAIHGIPPTVAIEQRASRGGLKSTVATLTEIYHFLRLIYVRLGTQYCPD
                     CRIAIAPQSPESIVAQMLRDYRGQRIQLLAPLVAHRKGHYRELAQWAAARGSKQLRVD
                     GKFVSTDPFPSLKRFVEHVIELPIGEVECKPRQETALRETVDAAIELGKGVLVALTTA
                     GASQTLSTRRACPGCGRGFPEPDPRLLSFNSSQGWCATCFGTGVQVENFEAEHTGEES
                     HWMAESTDEGQRSTEQVCDSCQGARLNPLALQLRFRERSIAQLTALPVRELSVFFDKL
                     RLGAREAAIARDAMAEICSRLGFLQRVGLGYLALDRAAPTLSGGEAQRIRLAAQLGSN
                     LQGVCYVLDEPTIGLHPRDNQVLLSALDQLSGAGNTLVVVEHDEDTIRRADHIIDLGP
                     GAGVRGGEVVACGSPQALERNPQSVTGRFLARPLQHPATPRRSTDARSAALEIESASL
                     HNLRDIDVRIPLGRLVVVTGVSGSGKSSLARDVLYANLRAIVGAKSAPRLIGVKAMRG
                     AERIARVLEVDQTPIGRTPRSCPATYVGFWDDIRRVFAGTSEARVRGYDAGRFSFNTS
                     GGRCPDCEGAGTRTIEMSFLPDVKIPCERCGGRRFDRETLEIQWRARSVGDVLAMNVE
                     EAVQFFAAHPRIARPLALLADVGLGYLTLGQQSPTLSGGEAQRIKLVAELARLTDSPR
                     ARPGHTLYVLDEPTVGLHMADVEKLIAVLHRLADAGHSVVVIEHNLDLMAEADWIIDL
                     GPEAGAGGGRVVAAGPPAAIAARKSHSHTGAALARFLLERAA"
     gene            6753..7058
                     /locus_tag="VNZ06_02765"
     CDS             6753..7058
                     /locus_tag="VNZ06_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19703.1"
                     /translation="MGHNLVCWKCGADLSALSLPLSRRDECKRCGADLHVCRLCSDYD
                     EHVAKKCREPIAEEVNDKTRANFCDYFAPRPGAYQPPDTSTAAKARSELERLFGPKT"
     gene            complement(7055..8716)
                     /locus_tag="VNZ06_02770"
     CDS             complement(7055..8716)
                     /locus_tag="VNZ06_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19704.1"
                     /translation="MNAAGSSFVINLCSSTSPVALAPPDHAGLKRFTFFVSRRREEGR
                     ERFRLHMGYFETQEEAEKLLDIVREIYPGAWAGLAPGQRLRAAAAAAAAQAPAAALGQ
                     EVAPARPRDSIATAALTAPPAAVAAKPEPSPAQLLAPSEPAAPVVTGPAAAKVPQLPE
                     QEVSFQLLPDDGARRRPDSIAAQPAHTLTASAKTPENPASTAVNAEAQAATRSLNNVR
                     AAIASLEDSTVHAPVLKPPADLKIPELKAAVIPVSRKPLEKPAAKPVEKPADKPVPAE
                     VEVLNDEAAMLVLESADSVAAKSTDGKAGVGPAAEKPCYAVQLIWSVKPIDVSQVPQL
                     AIFSAYTLYGAEGNRDGRRWYGLRLGFFTDAVSAKQVAHYVRSEFSTVSVVPVTTRER
                     ERAKNAARPGELALQTSPPAKPATAVIKPAPGTLVSTTQFEFIEDKAPAPSEASAAPQ
                     RNLPPRLGPAARTVRGAPGKRAKLRTPAQMAAKAAAAKPKKLTLEETLEILGAGDLKV
                     DDGRGELLNTQSAANAAAPKSSPANKGKSSRLGRLFERLSERIGN"
     regulatory      complement(8885..8969)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(9096..9782)
                     /locus_tag="VNZ06_02775"
     CDS             complement(9096..9782)
                     /locus_tag="VNZ06_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HWW19705.1"
                     /translation="MAEDAGRVALVAGSNGLVGSKLLPILLAAPEFGRIHALTRRALL
                     VDSPRLANRVVRFDAPLEAQLKGLQCSDAFCCLGTTMRTAGSQAAFRAVDHDLVIQFA
                     RLAHGAGAERFILVSSVGANAAAKSFYLRVKGETEKSLEALRFRSLNILQPSMLLGSR
                     RELRLLEVVAQPAMLIVNPLLIGKWSRYRAIGAETVAAAMYGTVRSQRKGVYRYTYDE
                     LRKLAANVQT"
     gene            complement(9832..11841)
                     /locus_tag="VNZ06_02780"
     CDS             complement(9832..11841)
                     /locus_tag="VNZ06_02780"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HWW19706.1"
                     /translation="MTGYKGRGALGNPPPRFLRTGIESVDDGWYREELPDSIATHVQP
                     EPARSIISRNDSPDIPFEQSINPYRGCEHGCIYCLAGDTRILMADGATQLLEQIQAGD
                     MIVGTAGGSTQRYYVRTQVLAHWSVIKPAYQITLEDGTQLVAGADHRFLTEQGWQFVV
                     PGDHGATRRAHLTAHSRLMGTGAFAAKPDHNADYRAGYLCGVTRGRGLALCATQAVER
                     AQGYLQQANVETECFSVRASAGSGHPFQAAWTQARLNVALVRELIAPRASPTSGWRAG
                     FLAGIYDADGSYAHGSLRFDPAADESQRLLRESLDSFGFRYIADGHALRVRGGLSEHL
                     RLLHSVDPAISEKRDISGRPVTSHPRLRVVSIESLGRAMRLYDMTTGTEDFIANGVVS
                     HNCYARPSHAYMDLSPGIDFETRIFYKEDAARLLEAELGAPGYVPKAITLGANTDPYQ
                     PLEKELRVTRSIVEVLERTRHPVSFITKGTLILRDLDLLRSLAADQLVHAFVSITTLD
                     AELKRIMEPRTASPQARLRLVRELTAAGIPTGVMVAPMIPMINDSEMEHIIEAAAQAG
                     AIQAGYVLLRLPHELKELFREWLDEHFPDRAEHVMSLIRAARGGRENDARFGARMMGT
                     GAWAQLLRDRFALACKRHGLVRGRLRSLATEHFRPPSRGGQMSLL"
     gene            complement(11929..12984)
                     /locus_tag="VNZ06_02785"
     CDS             complement(11929..12984)
                     /locus_tag="VNZ06_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19707.1"
                     /translation="MERKRARTLGLLCAFAATLAALSGCSDLAVALGLRVRLDKIPVT
                     GISARLVSNRDARVPVSALGPGQSARLIIVATDSAGKTYVTVGAGGGKVALNNYSIAT
                     EVAQIRSSKVSLSTDPTISEGKIGKLYIAPVAHPEVTTELDVPVRYDLAYRANFSAGP
                     GADGFDGASGISGSPGFDALQTTDPTTGLTSYQGAGGSGGDGTRGSDGGDGQDGSPGG
                     QVQVWMRLSDLDPSLLQVKAVSGSKQSLFLIDPNGGSLQITADGGSGGRGGRGGPGGQ
                     GGRGGAGSPSGLSGNNGLPGSDGRGGRVGAAGSIAISVDPAAESYLKALTWSNKSGDG
                     IPGPAPSITFAPVPPLW"
     gene            complement(12992..14221)
                     /locus_tag="VNZ06_02790"
     CDS             complement(12992..14221)
                     /locus_tag="VNZ06_02790"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW19708.1"
                     /translation="MRLPAALSVFRHRPYARYALCRFSASLAWQMFAVAVGWQVYALT
                     RDPLALGFVGLAEFIPFVCLVLVGGYVADHVDRRKILLAMWTTDMLGLTSVCLLTLAH
                     VRVVWPIYLAIAVFGATRSFWQPSLQALVPNLVPIDEFPRALSVNSMLFQTAAILGPA
                     LGGLLFLLGPEVVFGSCAGLFALIIVVGSGLPPQRAGQRCAGELPQQNADGRFLDGLR
                     YVLHHRAVLGVISLDLFAVLFGGATALLPIYASDVLHIGPPGLGLLRTAPSVGAAITA
                     VVLTLRPINRHAGAVMFAGVGLFGVCTLIFGESRSFVLSLAALCVLGAGDMLSVYIRG
                     MLVQLRTPDAIRGRVSAISSMFIGASNELGEFESGVTARWFGAVRAVLLGGVLTLGVV
                     ASWIGLFPELRKLDKLR"
     gene            complement(14226..15152)
                     /locus_tag="VNZ06_02795"
     CDS             complement(14226..15152)
                     /locus_tag="VNZ06_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWW19709.1"
                     /translation="MSDGSLPPAPASAPWAPYVMRRQPRIEMVPMRSGRIAVTHWGPG
                     KRPPIVMLHGMLDCAASYQFVVDCLPDDWDLAAVDWRGYGHSDDRPDLYWLPDNFADL
                     EVLLDRLTPNEPARVIGHSLGGTVATSHAGIRPGRYRWLVNIEGFGMPSRSHTPAPVR
                     ITRWLDELRRLASPKRYASLEELAVRVARRHPRLPQDRAHYLAWAWSREVEGGFEIAA
                     DPKHRLMQPLRLRREDLDECWSQIRCPMLLLLGSESEFLTRVGGDVELARWRQLVPHM
                     RVEFIAGAGHMVPHEQPERLASEIVRFVNAHP"
     gene            complement(15149..16675)
                     /gene="ilvA"
                     /locus_tag="VNZ06_02800"
     CDS             complement(15149..16675)
                     /gene="ilvA"
                     /locus_tag="VNZ06_02800"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HWW19710.1"
                     /translation="MTDDYLQRVLKARVYDVAIESPLELAPRLSRRLNNRILLKREDL
                     QPVFSFKLRGAYNKISHLSQVIAVRGVICASAGNHAQGVALAARKRGIPAVIVMPQTT
                     AAIKVQAVIDLGAEVVLHGDIYDSAYDHALTLARERHMVFVHPFDDPDVIAGQGTIGV
                     EILRQTAGELDAIFVPVGGGGLISGIAAYVKQIYPRIRIIGVQPEDAAAMYEAKQRGT
                     RVTLERVGLFADGVAVRRPGEETLELVRKYVDEILLVNTDEICAAIQDIFEDTRSISE
                     PAGALAVAGIKRYVERGKIHDQRLIAINSGANMNFDRLRHVAERADLGALREALLAVE
                     IPERPGSFLQFCEVLGQRNITEFNYRLQGSERARVFVGLALEHGHQEAENIIAQLRTA
                     GYTVQDMIGNEMAKLHIRFMIGGRAPPSESNLEELLYRFEFPERPGALLRFLKAIGNS
                     WNISLFHYRNHGSDYGRVLAGIQVSKGEREEFLRHLHDLHYPYVDETANPAYKMFLAP
                     "
     gene            complement(16729..18270)
                     /locus_tag="VNZ06_02805"
     CDS             complement(16729..18270)
                     /locus_tag="VNZ06_02805"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWW19711.1"
                     /translation="MAKQKETPTAADLDETAELPIIPGGAGVSVSEDPLVATDAYNAP
                     TVFRAPPAIDDIREARIAQREAEIGALRSDLASVTESRGQLENNLGNLTTNLRDLEQM
                     LNTKSEQLSMFEREVGQRDRRISELAARNATLDGELAAKITERDSLRVERDSLRVELQ
                     AVRADIESSHQRISELEARTATLDGALAATTAERDTLRLDLRVAREDADSSRQLIKAR
                     DASEGALLKEREQQNRRVRQLETDIAQARANAERYRERLQSMEGRRQIFASMLDEREA
                     EVIERGTTLVKLESEMTQRTRFSAARESDLKGLLSAEQERVRQLEQAAIISRTEQESL
                     RLSLTEAARAADERVASVRAELESQQAAMAELRAQLAAVNSSLDERNALIERLETEMQ
                     SSSQVLGSIQHNLQHLGDAEPTRMLVRTQGETGIVHMLGRRTTLGRTPDNDIRIDEDF
                     ISRHHAVLLMSGLNTIVEDLNSTNGTYVNGERINRRTLKEGDLVTLGKTEFRFVVKKA
                     SDKPA"
     gene            complement(18280..18804)
                     /locus_tag="VNZ06_02810"
     CDS             complement(18280..18804)
                     /locus_tag="VNZ06_02810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWW19712.1"
                     /translation="MRRAYFDCRFGQLHVHYAIPAGGGFDEATTLLCIPGAPGLGRFF
                     RPLLLPLGRDRSVYAPDLPGSGESDPAPGMLDATSAAAALGDFLDWMHLRQVDLLAHE
                     GGVNIALALASQRSAQVGRVVLSPNTDAVRADARSLRQPQLVIDLRPADAGGHNAAEL
                     ERQAAQLRDFLGLA"
     gene            19134..>20572
                     /locus_tag="VNZ06_02815"
     CDS             19134..>20572
                     /locus_tag="VNZ06_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19713.1"
                     /translation="MAAMPLLRPLVLASALLGAALTVSAGNAESTDAGLPPDAELEAA
                     HAHIGKIEVRTLQIFDLGDPADNYWLFRTADHLHQRTRNSVILAQLLFRSGDPYSRRI
                     LDESARNIRLNSSFLREPVIHPIRYHDGLVDIEVITHDVWTFEPGVNFSRSGGANTTG
                     VDIADANFFGFGKYIEIGHGQNVDRSSNFLRWQDPNVWGSHWTDGAVYSNNSDGTVWQ
                     LVGGLPFYSLESHAAAGVDLLDNHSIVTRYRLGRTYDGYDVNDHAGDLFVGRAIVVTD
                     AWTERMLLGWRVDNDNFSAPPGQTPLAALPQDRDLSYPFVRMQWVENRFDTMRNLDLI
                     ARTEDVHFGLDASIGLGLATPALGADRHSVLADTEINYGWRFSETEEMFLTSRLASRF
                     EDGGLRDAMITWGAAYYLQTSEMTRMLVRFTGDAGHDLDGDHTVQLGGDTGLRGYPLR
                     YQNGNQRALFTLEERLYTNWYLFRLFNVGA"
BASE COUNT         3716 a         6690 c         6448 g         3718 t
ORIGIN      
        1 ggatgatgcg gccctcatgc tcggtgatcg agcagaaagt acagccgccg aaacagccac
       61 gctggatcgc caccgagaag cgaatcatct tgtaggctgg aatgagtgcc tcgccatagg
      121 cgggatgcgg tctgcgctga aaaggcaggt catagatcca gtccatctcc gcgctggtga
      181 gcggaatggg cggcggattc agccacacct gctgtgagcc atgcgcctgc accaaagcgc
      241 gggcgtttcc gggattggac tccagatgca ggatgcgcga ggcatgggcg tacatcaccg
      301 gatcgtcgga cacctgctcg aacgccggca gccgaatgac gctgcgttcg cgatcggcgt
      361 tgcgcacccg ccgcacgaaa cgcacgacct gcgcaccgtc gccgcaggcc tgtggtgcac
      421 tcgcggcccg aggttccgcg gcgtatggat ccacgggcgg catcagcgct ccgggtgcgt
      481 ccaggtgagt cgaatcgatc tcgatccagt cagcgcacat cgagcgctgg atgaacgcgg
      541 taccgcggat gtcctgcagc gcctcaatgc gctcgccggc gtcgagtcgg cgcgcgatct
      601 cgatgatctg tcgctcgccg ttgccataca ccagcagatc cgctttcgcg tccagcagga
      661 tcgagcggcg cactttctcg gaccagtagt cgaagtgcgc gatgcgtcgc agcgaggcct
      721 cgatgccccc aatcacgatc ggcacgtcgc gccaggcttc gcgcgcgcgc tgcgcataga
      781 cgatgaccga gcgatccggt cgtctgccgc cctccccacc gggagtgtac gcatcatcgc
      841 tgcgcacccg tcggtccgcg gtgtagcggt tgaccatcga gtccatgttg ccggcggtga
      901 taccgaagaa caggcgcggc ttgcccagga cgcgtaggcc ttctgcgctg tgccagtccg
      961 gctgcgcgat cacaccgacc ttcagcccct gcgcttcgag cacccggccg atgatcgcca
     1021 tgccaaaact gggatgatca acgtaggcat cgccggagac gaccacgaca tcgcactgat
     1081 cccagccgag cgcctgcatc tgcgcgcgcg aagtaggcag gaatggtgcg gcggcgaagc
     1141 gcggcgacac ccgggcggaa cgaaggtcag gggcggctaa catggcaccc cattatagcc
     1201 ggcggtgtca atagcccgac gcacttgtcc attgccgttc ggattcacat aaaatccgct
     1261 catcatgagc ccgcgtgcgc atgacgcgat cgtcgtgcgc ggcgcgcgtc agaataacct
     1321 tcgcgatctc gatctggacc tgccgctcaa cgagctgatc gtcgtgaccg gtgtgtccgg
     1381 ctcgggcaaa tcctcattgg ttttcgacac cctgtacgcg gaaggccagc gccgttatgt
     1441 cgagaccttc tcgccctacg cgcgccagtt tctcgaccgc atggacaagc cgcaggtgga
     1501 ccgcatcgag cgtatcccgc ccgcgattgc catcgatcag gtcaacccgg tgcgcacgtc
     1561 gcgttccacc gtcggcacga tgaccgagct gaacgatcat ctgaagttgc tgtttgcgcg
     1621 cgcggcgcgt cttgactgcc gccagtgcca caagccagtg cggcgcgaca acgcgcagag
     1681 catcgctgac gcgttgcctg ggctgctggc cgaattcgag cgtccgctgc tgctgatctg
     1741 tttcccggta ccgctgccag cgcagctgaa gatggcggag ttgaaagcgg agctggaaaa
     1801 gctcggctac gttcgcatcc atcaggagca gcccaagctc ctgacgatga ttcaggatcg
     1861 cctgcgcctg cagggcgagg atcgcagccg actggtcgag gcgctcgaag ccgcgctgcg
     1921 atccggtcac ggccgcgtca gcattcatgc cctggatacc aagggcgcgg agcgcgcgag
     1981 ccttcgattc tcgaacgacc tgcattgcgc tgactgtgac ctgcactacc aggacgcgac
     2041 ccagggattg ttctcgttca attcaccctt gggcgcctgc gatacctgtc gtggcttcgg
     2101 tcgcatgatc gggatcgact atggcctagt cgtgcccgat accggcaagt cgctcgctgg
     2161 cggagccata cgaccgtggc agaccccctc ctacaaggaa tgtcaggacg atctgctgcg
     2221 ctttgggaaa aagcgtggca ttgcgctcga caccccgtgg cgcgagctga gcgatgaaca
     2281 gcgccgctgg gtcatcgagg gcgagggagc gtgggagaag aaagtctggt acggcgcgcg
     2341 ccgctacttt gcctggctcg agaccaaagc ctacaagatg cacatccgcg tgctgctgtc
     2401 gaagtatcga agctacacgc tgtgccatac ctgccagggc gcccgcctca aaccggacgc
     2461 cttgctgtgg cgtctgggcg attctcacgg gctggcaatc cacgaggtca tgcagctacc
     2521 gatcgagcgc tgccacgaat tcttcctgca cttgagactg ccgaaaccgc tcgatcaggc
     2581 ggccgatctg ctgctgggcg aactgcgtac gcgattgcgg tacctgtgcg atgtcggcct
     2641 gggttatctg acgctggatc gtcaatcgcg cacgctgtcc ggcggcgagg tccagcgcat
     2701 caatctgacc acggcgctcg gtacctcgct ggtgaacacg ctgtttgtcc tggacgagcc
     2761 ctcgatcggg ctgcacccgc gcgacatgca gcgcgtgatc ggagtcatga agcgcctgcg
     2821 cgacgccggc aacacgctgc tggtcgtgga acacgatccg cagatcatga tcgaggccga
     2881 ccgcattctc gatctggggc ccggtgccgg cgaacacggc ggatcgatcg tctgctacgg
     2941 cacgccgcgc gaagtgatgg tccatccgag ctcggccacc ggagcgtggc tggcgggcag
     3001 acgccgtttg ccgccgctgc acgcaacggc cgattcaccc cggcggacgg gtaatgcggc
     3061 gacgcttcgc ctgaccggcg ccgccgaaca caatctcaag cagctcgatg tcgacatacc
     3121 gctgcatcgg ctggtatgcc tgacgggggt ctccggctcg ggcaagtcaa ccctggttca
     3181 ggatgtgtta tttccagcgt tgcagaaggc gcacggcaag ccgagcgaat cgcccggtgc
     3241 cttccggact ctctcgggtg cggagctgat tgatgcggcc gtgctcatcg accagacacc
     3301 gatcggccgc accacgcgtt ccaatccggc cagctacgtc ggcgcattcg atgagattcg
     3361 cgcccgcttc gcgcaggaac cggaatcccg gctgcgccgc tacaccgcag gaaccttcag
     3421 cttcaatagc ggcaatggcc gctgcccggc atgcggcggc aacggtttcg agcatgtgga
     3481 gatgcagttc ctgtcggatg tgtatctgcg ctgtggcgaa tgcaacggtt cgcgctatcg
     3541 cagcgagacg ctgcaggtgc gcatcgcggg tgccaataac aggcgcgccc acatcgcgca
     3601 agtgctcgat atgactgcga ccgaggcgct gacgttcttc agcgacgtga ctgcgatctg
     3661 tgcgcggtta cagccactgg tagacgtcgg tctggactac gtgcggttgg ggcagccggt
     3721 accgacgctg tcgggcggtg aggcgcagcg ccttaaactg gctggacacc tggcgcaggc
     3781 cgcgctcggc aacggcagga acagccggga aaaggacaaa ccctcgctcg gcaagctgct
     3841 gatcttcgat gagcccacca ccggattgca cttcgaggac atcgcgcggc tggtgagcgc
     3901 attcggcctg ctattgcagg ccggtcactc gctgctggtg atcgagcaca atctggacgt
     3961 cattcgagcg gcggactggg tcatcgatct cggtcccgaa gggggtgatc gcggcgggga
     4021 aatcatcgcc gctgcgaccc cgggcgatct gatgcggatt gcgcgatcgc acaccggccg
     4081 ggcgctgtct cagtatgcgc aggcgcagta caccgtcgcc gagcccagcg gatcctatga
     4141 ggccgcgacc gtgacacccg ctcacggcag cgtgacggtg cgcggggctc gcgagcacaa
     4201 cctcaagaac atcaacgtcg agattccgca cgagcgcttc accgtgatta ccggggtatc
     4261 cggctccggc aaatcaaccc tggctttcga cctgctcttc aacgagggcc agcgccgcta
     4321 cctggaatcc ctgaacgcct acgcgcgcca gttcgtacag ccggcagcgc gtcccgacat
     4381 ggatgcgatt cacggcattc cacccacggt cgcgatcgag cagcgcgcca gtcgtggcgg
     4441 gctgaagagc acggtcgcga cactcaccga gatctaccat tttctgcgac tcatctatgt
     4501 gcgtctggga acgcagtact gcccggactg tcgtatcgcc atcgcgccgc aaagtcccga
     4561 gtcgatcgtg gcgcagatgc tgcgcgacta tcgcggccag aggattcagc tgctggcccc
     4621 attggtggca catcgcaaag gccactatcg cgagctggca cagtgggccg cggcgcgcgg
     4681 cagcaaacag ctgcgcgtgg acgggaaatt cgtcagcacc gacccgttcc cgagcctgaa
     4741 gcgttttgtg gaacatgtga ttgagttgcc gatcggtgag gtcgagtgca agccgcggca
     4801 ggaaaccgcg ttgcgtgaaa ccgtggatgc ggcgatcgaa ctgggcaaag gcgtgctcgt
     4861 cgcgctcacc accgcgggcg ccagccagac cctctccacc cgcagagcct gccccggctg
     4921 cggccgcgga ttcccggaac ctgacccgcg cctgctgtca ttcaactcca gtcagggctg
     4981 gtgcgctacc tgtttcggca cgggcgtgca ggttgagaac ttcgaagccg agcacaccgg
     5041 tgaggaaagc cactggatgg cggagtcgac cgacgagggt cagcgcagca ccgaacaggt
     5101 ctgcgacagc tgccagggcg cccgcctgaa tccgctggcg ctgcagctgc gttttcgcga
     5161 gcggtcgatc gcgcagctga cagcgctacc ggtcagggag ctgagtgtgt tcttcgacaa
     5221 gctgcgctta ggcgcccgcg aagcggcgat cgcacgcgat gcgatggcgg aaatatgcag
     5281 ccgactcggg tttctgcagc gtgtgggcct gggctacctg gcgctcgatc gcgccgcgcc
     5341 gacgctctcc ggcggcgaag cgcagcgcat acgtctggcc gcgcagctcg gctccaacct
     5401 gcagggggtt tgctatgtgc tcgatgagcc gacgatcggg ctgcatccac gcgacaatca
     5461 ggtgctgctg agcgcgctcg atcaactctc cggcgccggc aacacgctgg tggtcgtgga
     5521 gcacgacgaa gacacgatcc gtcgcgccga tcacatcatc gacttaggtc ctggcgccgg
     5581 cgtgcgcggc ggcgaagtcg tagcctgcgg ctcgccgcaa gcactcgagc gcaatccgca
     5641 atcggtcacc gggcgtttcc tggcccggcc gctgcagcat ccggccactc cacgccgcag
     5701 caccgatgca cgcagtgcgg cgctggaaat cgaatcggcc agtttgcaca acctgcgcga
     5761 catcgatgta cgcattccgc tgggccgact tgtcgtggtc accggtgtgt cgggctctgg
     5821 caagtcttcg ctcgcgcgcg acgtgctcta tgccaacctg cgcgccatcg tgggcgcaaa
     5881 gtcggcaccg cggttgatcg gtgtcaaggc gatgcgtggc gcggagcgga tcgcccgtgt
     5941 gcttgaggtc gatcaaacac ccatcgggcg cacgccccgc tcctgcccgg ccacctatgt
     6001 cggtttctgg gatgacatcc ggcgggtgtt cgccggcacc agtgaagcgc gcgttcgcgg
     6061 ctatgacgcc gggcgcttct cctttaacac cagcggtggc cgctgtccgg attgtgaagg
     6121 cgccggcacc cgcaccatcg agatgagctt cctgccggac gtcaagattc cgtgcgagcg
     6181 ctgtggtggc agacgcttcg atcgcgagac cctggaaatc cagtggcgcg ctcgctcggt
     6241 cggcgacgtg ctggcgatga acgtcgagga agccgtgcag ttcttcgcag cgcatccgcg
     6301 cattgcgcgc ccgctggcgc tgctggccga tgtggggctc ggctacctga cgctgggtca
     6361 gcaaagccca accctgtcag gcggtgaagc gcagcgtatc aagctggtgg ccgaactggc
     6421 gcgtctgacc gactcaccgc gcgcgcgccc gggccatacg ctctatgtgc tcgatgagcc
     6481 gactgtcggt ctgcacatgg cggacgtgga aaaactgatc gccgtcctgc atcggctggc
     6541 cgatgcaggc cattccgttg tggtgatcga gcacaatctg gatctgatgg ccgaagcgga
     6601 ctggatcatt gatctcggtc cggaagcggg ggccggaggc ggtcgcgtcg tggcggcggg
     6661 tcctccggcg gccattgcgg cccgtaagtc gcactcgcat actggtgcgg cgctggcgcg
     6721 tttcctgctc gaacgcgccg cgtagtcgcc atatgggtca caacctggtt tgctggaagt
     6781 gcggcgcgga cctgtcagcc ctgtcgctgc cgctgtctcg ccgcgatgag tgcaagcggt
     6841 gcggcgcgga cctgcacgtg tgtcgcctgt gcagcgacta cgatgagcac gttgccaaga
     6901 aatgccgcga accgatcgcg gaagaagtca acgacaagac acgggccaat ttttgcgatt
     6961 acttcgcacc gcggccgggt gcctatcaac cccctgacac cagcactgca gccaaggcgc
     7021 gttcggaact cgaacggctg tttggaccga agacctagtt cccgatccgc tctgacagcc
     7081 gctcgaacag ccggccgagt cgtgaggact tgcccttgtt cgccggggag gacttaggag
     7141 cggcggcatt cgcagcggac tgggtattga gcaattcacc gcgaccatcg tcgactttca
     7201 gatcgccggc accgaggatt tcgagcgtct cttcgagcgt cagcttcttg ggtttggcgg
     7261 ccgcggcctt ggccgccatc tgcgccggtg tgcgcagctt cgctcgcttg ccgggagcac
     7321 cccggacggt acgcgcggct ggacccaggc gcggcggtag attcctctgc ggcgcggcgg
     7381 aggcctcact cggtgcaggc gccttgtctt cgatgaattc gaactgagtc gtggacacca
     7441 gcgtgcccgg cgccggcttg atcacggcgg tcgcaggctt ggctggcggc gatgtttgca
     7501 gcgccagctc cccgggccgc gcggcgttct tggcgcgctc gcgttcgcgc gtcgttaccg
     7561 gaacgaccga cacggtcgag aattccgagc gcacgtagtg cgccacctgc ttggcggaca
     7621 ccgcatcggt gaagaagccc agtcgcagac cataccagcg acgcccatcg cgattgcctt
     7681 cggccccata gagcgtatag gcactgaaaa tcgcgagctg cgggacttgc gacacatcga
     7741 tcggtttcac cgaccagatc agctgtaccg catagcaagg cttttctgcc gccggtccaa
     7801 cccccgcttt gccatcggtg gactttgcgg cgactgagtc ggccgactcg agcaccagca
     7861 tcgcggcctc gtcgttgagc acttcgacct cggcaggtac cggcttgtcg gcgggctttt
     7921 caactggctt cgcggccggc ttctcgagcg gctttcgtga gaccggtatc accgccgctt
     7981 tgagttcggg aatcttcagg tccgcgggcg gcttcaatac cggcgcgtgc accgtgctgt
     8041 cctcgagcga ggcaatcgcc gcacgcacat tattcagtga gcgcgtagcg gcctgcgcct
     8101 cagcattgac tgccgtgctg gcagggtttt ccggcgtctt ggcagacgcg gtgagggtat
     8161 gagcgggttg ggctgcgatc gaatcgggac gccgccgcgc accgtcatcc ggcagcagct
     8221 ggaacgacac ctcctgttcg ggcagctgcg gaaccttggc cgccgccggc cccgtgacca
     8281 caggcgcagc gggctccgaa ggagcaagca gctgagcggg tgacggctcg ggcttcgccg
     8341 ccacggcagc cggtggggct gtaagcgccg cagtcgcgat gctatcgcgt ggccgtgccg
     8401 gtgcaacttc ctgtccgagc gccgctgccg gggcctgagc cgcggcggcc gccgccgcgg
     8461 cgcgcaagcg ttgcccgggt gccaggcccg cccatgcgcc agggtagatt tcacgcacga
     8521 tgtcgagcag cttttccgcc tcttcctggg tttcgaaata acccatgtga aggcggaagc
     8581 gctcgcgtcc ttcttcacga cgacgcgaga cgaagaaagt gaatcgtttg agaccggcgt
     8641 gatccggtgg tgcgagtgcg accggcgagg tggaggaaca tagattgatg acgaatgagc
     8701 tgcctgccgc gttcatcggc atcgcgcctg tcggagtcgc tgctttcagt tgctctttaa
     8761 tctgcgtcgc ctgctgcatt cgcgttgatc cccgattgcc catacggccc aaatcaacac
     8821 ggcactcgga aacatcgcgg ctgacttcag cgacagatgt gcggcttgac gcgcgcatcg
     8881 tagcttcggc aaccccgcta tcccttccct gtttctaagg accggtggct ttgcgtcccc
     8941 gccttgcgac gggtttgctc ttaaaagtac agaatcagtg tgacagttca aagcgacaaa
     9001 tcaagactga agttctcgaa attcgtcacg ctgtcgcacg attgcagcgc gtaatataaa
     9061 ttctgttaaa tacggaacgt gcgccgcaac aggatttatg tctggacgtt cgccgcgagt
     9121 ttgcgcagct cgtcataggt gtaacgatag acccctttgc gctgactgcg caccgtgccg
     9181 tacatggctg cggccactgt ttctgccccg attgcccgat aacgcgacca tttcccaatc
     9241 agcagcggat tgacgatgag catcgccggc tgcgccacca cttcgagcag ccgcagttca
     9301 cggcgcgagc cgagcagcat cgacggctgc aggatgttga gcgagcgaaa tcgaagtgct
     9361 tccagcgatt tctcggtttc gcccttgacg cgcagataaa aactcttcgc cgccgcattg
     9421 gctccgacgg acgacaccaa tatgaatcgc tcggcgcccg cgccatgcgc aagtcttgcg
     9481 aactggatca caagatcgtg atccaccgca cgaaacgcgg cctggcttcc cgcggtacgc
     9541 atcgtggtgc ccaggcagca gaacgcatcg ctgcattgca ggcccttgag ctgcgcctcg
     9601 agcggtgcgt cgaatcgcac cacgcgattt gccaaacgcg ggctatcgac cagcagcgct
     9661 cgccgcgtca acgcatggat gcgtccgaac tccggagccg ccagcaaaat cggcagcagt
     9721 ttgctgccga caagcccatt gctgcccgcg accagagcga ctctgcctgc atcttctgcc
     9781 atgcgcgtga agcttacggt cgtcgatcgc tccgatcaac ggtacgcgca cttacagcag
     9841 cgacatctgc ccgccgcgtg acggcggcct gaagtgctcg gtggcgagac tgcgcagacg
     9901 tccccggacc aacccgtggc gtttgcacgc aagtgcaaat cgatcacgca acagctgtgc
     9961 ccaggctccg gtacccatca tgcgagcgcc gaatcgcgca tcgttctcgc gaccgccgcg
    10021 cgccgcgcga atcagcgaca tcacatgctc cgcacgatcc gggaaatgct cgtcgagcca
    10081 ctctcggaac aactccttca gctcatgcgg cagccgcagc aacacatagc ccgcctggat
    10141 cgcgccggct tgcgcggccg cttcgatgat gtgctccatc tcggaatcgt tgatcatcgg
    10201 gatcatcggc gcgaccatca cgccggtggg tatgcctgcc gcggtcagct cgcgtaccag
    10261 gcgcagccgt gcctgcgggg aggcggtccg cggttccatg atgcgcttga gctcggcgtc
    10321 cagcgtcgtg atgctcacga acgcgtgcac cagctgatcg gctgccagcg agcgcagcag
    10381 atcgagatcc cgcaggatca gcgttccctt ggtaataaag ctcaccggat ggcgggtgcg
    10441 ctccagcacc tccacgatcg agcgcgtgac gcgcagctct ttttcgagcg gctggtaggg
    10501 atcggtattg gcaccgagcg taatggcttt gggcacgtaa ccgggtgcac ccagttccgc
    10561 ctccagcaag cgcgcagcgt cttccttgta gaagattcgg gtctcgaaat cgatccctgg
    10621 cgacagatcc atgtaggcat gactcggccg tgcatagcag ttgtgactga cgacaccgtt
    10681 ggcgatgaaa tcctcggtgc ccgtcgtcat gtcgtacagg cgcatcgcgc ggccgagcga
    10741 ttcgatcgac acgacccgca atcggggatg actcgtgacc ggccgcccgg aaatgtcgcg
    10801 tttctcggaa atcgccgggt ccacgctatg caacagcctc aggtgctcac tcaatccgcc
    10861 acgcacgcga agcgcatggc cgtccgcgat gtagcggaaa ccaaaactgt ccagtgactc
    10921 acgcaacaga cgttgcgatt cgtccgcggc gggatcaaag cgcagcgaac cgtgcgcata
    10981 gctgccgtcc gcgtcataga ttccagccag aaagccggcc cgccagccac tcgtcggcga
    11041 tgcgcgcgga gcgatgagtt cgcgcaccag cgccacattc aaccgtgcct gggtccaggc
    11101 ggcctggaag ggatgcccac tgcctgcgga tgcgcgtacg ctgaagcatt cggtctccac
    11161 atttgcctgc tgcagatacc cctgcgcgcg ctcgaccgcc tgcgttgcgc acagcgccag
    11221 accacgacct cgcgtgacac cgcacaggta gcccgcgcga tagtccgcgt tgtgatccgg
    11281 cttcgcggcg aacgcgccgg tgcccatgag acggctgtgg gcagtcaggt gcgcgcgtcg
    11341 cgtcgcaccg tgatccccgg ggacgacgaa ttgccagccc tgttcggtca gaaaccgatg
    11401 atccgctccg gcgaccagct gcgtgccgtc ttccagcgtg atttgatagg ccggcttgat
    11461 gacgctccag tgcgccagga cttgcgtcct cacgtagtac cgctgcgtcg agccgcctgc
    11521 ggtgccgacg atcatgtcgc ccgcctgaat ctgctcgagc aactgggttg cgccatccgc
    11581 catcaggatg cgcgtgtccc cggccaggca ataaatgcat ccgtgctcgc acccgcggta
    11641 agggttgatc gactgctcga aggggatgtc gggtgagtcg ttgcgggaaa tgatcgagcg
    11701 ggccggctcg ggctgcacgt gagtggcgat cgagtctggc agctcctccc gataccagcc
    11761 gtcatcaacc gactcgatgc cggtgcgaag aaaccgcggc ggcgggttcc cgagcgctcc
    11821 acgaccctta tagccagtca tcgtgcagat actgtacatc catacagttc tccgggtcca
    11881 tagaggtgcc accaaaaatg gaacgcctca gcgatcgcac catccccctc accacaacgg
    11941 cgggaccggc gcgaacgtga tgctgggagc tggaccgggt attccgtcgc ctgacttgtt
    12001 cgaccaggtc agggccttga ggtacgactc agccgcggga tccaccgaaa tcgcgatcga
    12061 gccggccgcc cctacccgac ccccgcgccc atcggagcct ggcaagccat tgtttccgct
    12121 cagaccgctt gggctaccgg cacctccccg gccaccttgc cctccgggac ctcccctgcc
    12181 gccgcggccg ccggatccgc catcagccgt gatctgcagc gatcctccgt tgggatcgat
    12241 gagaaacagc gattgcttgc tgccgctgac cgccttgacc tgcagcaagg acgggtccaa
    12301 gtccgacaga cgcatccaca cctgcacttg cccgcccggc gagccgtcct gtccatcgcc
    12361 gccgtcgctg ccgcgggtac cgtccccgcc gctacctccg gcgccctgat aggaagtcag
    12421 gccggtcgtt ggatccgtgg tctgaagcgc atcgaatccg gggctaccgc tgattccgga
    12481 cgcgccatca aatccatcgg cacctggccc tgccgagaaa ttcgcgcgat aggcgaggtc
    12541 atagcgcacc ggaacatcaa gctcggtcgt gacctcggga tgcgcgactg gcgcgatgta
    12601 aagttttccg atcttgcctt cactgatggt ggggtcggtc gacagcgata ccttggaact
    12661 tcggatctgc gcgacttcgg tggcgatgct gtagttgttg agtgcgacct tgccgccgcc
    12721 ggccccgacc gtgacatacg ttttgccggc cgagtcggtc gcaacgatga tcaggcgggc
    12781 cgattgtccg ggacccaagg cactgaccgg caccctggca tcgcgattgg acaccagacg
    12841 cgccgagatg cctgtgacag gaatcttgtc caaccggacc cgaagtccca acgccaccgc
    12901 cagatccgag catcccgaca acgccgcgag cgtcgccgcg aacgcacaca gcagaccgag
    12961 ggtccttgcg cgcttacgct ccattgcagt gtcatctcaa cttgtcgagt ttcctgagtt
    13021 ccgggaacaa tcctatccag cttgccacga caccgagcgt caacacgcca cccagcagca
    13081 ctgctcgcac cgcgccgaac cagcgcgccg tgactcccga ctcgaactcg ccgagctcat
    13141 tcgaagcgcc gatgaacata gagctgatcg cgctgacccg accgcggatc gcatccggcg
    13201 tgcgtagctg taccagcatg ccgcgaatgt agacgctgag catgtctcct gctcccagaa
    13261 cgcacaacgc cgccagcgag agtacaaagc tgcgcgactc accgaagatc aaggtacaga
    13321 cgccaaacag tccgacgccg gcaaacatga cggccccggc gtgccggttg atcggtcgaa
    13381 gggtcaagac caccgcagtg atggccgccc ctacactggg tgcagtgcgc agcaacccca
    13441 gtccgggcgg accgatgtgc agcacgtcgc tggcgtatat cggcagcaag gcggtcgcac
    13501 caccaaacaa cacggcaaac agatccagcg agatcacgcc gagcaccgca cggtggtgca
    13561 gcacatagcg caggccgtcg agaaagcggc catccgcatt ctgctgcggc aattcgccgg
    13621 cacagcgttg tcccgcccgc tggggcggaa gacccgaccc gacaacaatg atcagcgcaa
    13681 acagtcccgc acagctgccg aatacgactt ccggtccgag cagaaacagc agtccgccca
    13741 gtgccggacc gaggatcgcc gccgtctgga acagcatcga gttcaccgac aacgcgcgag
    13801 gaaactcgtc gatagggacc agattgggga ccagcgcttg cagtgagggc tgccagaacg
    13861 atcgtgtcgc accgaaaacg gcgatcgcaa gatagatcgg ccacacgaca cgcacgtgcg
    13921 ccagtgtcag cagacacacc gaggtcagcc ccagcatgtc ggtcgtccac atcgcgagca
    13981 gtattttgcg tcggtcgaca tgatcggcca cgtagccgcc gaccagtacc aggcatacaa
    14041 acgggataaa ttcggcgagc ccgacgaagc cgagcgccag cgggtcccgg gtcagcgcat
    14101 acacttgcca tccgacggcg accgcaaaca tctgccatgc cagggatgcc gagaaccggc
    14161 acagcgcata gcgcgcatag ggtcgatgac gaaagacgct cagtgccgcg ggaagccgca
    14221 ctgccttagg ggtgggcgtt gacgaagcgc acgatttcgc tggccagtcg ttcgggctgt
    14281 tcgtgcggca ccatgtgtcc ggcgcccgca atgaactcga cccgcatatg tggaacgagc
    14341 tggcgccagc gcgcgagttc cacatcgccg ccgacacgcg tcagaaattc cgactcggaa
    14401 ccgagtagca gcagcatcgg gcatcggatc tgtgaccagc attcatccag atcctcacgc
    14461 ctcaggcgca gcggctgcat caggcgatgc ttgggatcgg ctgcgatttc aaagccaccc
    14521 tccacttcac gcgaccaggc ccacgccagg tagtgagcgc gatcctgcgg cagacgcgga
    14581 tgacgtcggg ccacgcgcac cgccagttcc tcgagcgatg cgtagcgctt gggcgaggca
    14641 agtcttcgca gttcatccag ccagcgcgtg atacgcaccg gagcgggcgt gtgcgaacga
    14701 gacggcatgc cgaagccttc gatgttgacg agccagcgat agcgtccagg acgaatgccg
    14761 gcgtgactgg tggcgaccgt tccgccgagg ctgtgtccga tcacccgtgc cggctcgttc
    14821 ggcgtcaatc gatcaagcag cacttcaaga tcagcgaagt tgtcgggcag ccagtagagg
    14881 tcgggacgat catcgctatg gccgtagccg cgccagtcca ccgccgcgag atcccaatca
    14941 tccggcagac aatccaccac gaactgatag cttgccgcgc aatcgagcat gccgtgcagc
    15001 atgacgatcg gcggacgttt gcccggtccc caatgcgtga ccgcgatgcg gccgctgcgc
    15061 atcggcacca tttcgatcct gggctgccgg cgcataacgt acggcgccca cggcgctgat
    15121 gcgggcgcag gcggcagtga accatcactc atggcgcaag aaacatcttg taggcggggt
    15181 tggcggtctc atccacatag ggatagtgaa ggtcgtgcag gtggcgcagg aattcttcgc
    15241 gttcgccctt cgacacctga atgccggcca gcacacgccc gtagtcggaa ccgtgattgc
    15301 gatagtggaa caggctgatg ttccaggaat tgccgatcgc cttcaggaat cgcagcaggg
    15361 ccccaggccg ctccggaaac tcgaaacgat acagcagctc ttcgagattg gattcgctcg
    15421 gcggtgcccg cccaccgatc atgaagcgta tgtgcagctt cgccatctca ttgccgatca
    15481 tgtcctgcac ggtgtagccg gcggtgcgca gctgcgcaat gatgttctca gcctcctgat
    15541 ggccatgctc gagcgccagt ccgacgaaca ctcgtgcgcg ctcgctgccc tgcaggcggt
    15601 agttgaactc ggtgatattg cgttggccaa gtacctcgca gaattgcagg aagctgcccg
    15661 gtcgttccgg gatctccacc gccagcagtg cttcacgcag cgcacccaga tcggcgcgtt
    15721 cggccacgtg ccgcagacgg tcgaagttca tgttggcgcc gctgttgatc gcgatcaggc
    15781 gctggtcgtg aattttgccc cgttcgacgt agcgtttgat gccggcgacg gccagcgcac
    15841 cggccggttc ggagatcgag cgcgtgtctt cgaagatgtc ctgaatggca gcgcagatct
    15901 catcggtatt caccagcagg atctcatcca cgtacttgcg gaccaattcc agcgtctctt
    15961 cgcccggtcg ccgaaccgcg acgccgtcgg caaacaggcc cacccgctcg agcgtgaccc
    16021 gcgttccgcg ctgcttggcc tcgtacatcg cggcagcatc ctcgggctgt acgccgatga
    16081 tgcggatgcg cggatagatc tgcttgacgt acgcggcaat gccgctgatc aatccgccgc
    16141 cgcccaccgg cacgaagatt gcgtccagtt cgcctgcggt ctggcgcagg atttcgaccc
    16201 cgatggtgcc ctgaccggcg atgacgtccg gatcgtcgaa cggatgcacg aacaccatgt
    16261 ggcgctcgcg cgccagagtg agggcatgat cgtaagcgct gtcgtagata tccccgtgta
    16321 gcaccacttc cgcgcccaga tcgatcactg cctgcacctt gatcgcggcc gtggtctggg
    16381 gcatgacgat gacggccgga atgccgcgct tgcgcgccgc cagcgcgacc ccctgggcat
    16441 gattgccggc ggacgcgcag atcacgccgc gcacggcaat gacctgcgac aggtgcgaga
    16501 tcttgttgta agcgccccgc agcttgaagg agaacaccgg ctgcaggtct tcgcgtttga
    16561 gcaagatgcg attgttcagt cgccgggaca ggcgcggcgc gagttccaat ggcgactcga
    16621 tggcaacgtc gtagacgcgc gccttcaata cgcgctgcag atagtcatcg gtcatcgcgt
    16681 caggaacact ggaagcttgg atgcgacagc ttaagccatt cctgggcgct acgcaggctt
    16741 gtcggaggcc ttcttgacta cgaagcggaa ttcggttttg ccgagcgtca ccaggtcgcc
    16801 ctccttcagg gtccggcgat tgatgcgctc accgttgaca taggtgccgt tggtgctatt
    16861 gaggtcctcg acgatcgtat tgagcccgga catcagcagc acggcatggt gccggctgat
    16921 gaagtcctcg tcgatgcgga tgtcgttgtc gggcgtgcga ccgagcgtgg tgcgtcggcc
    16981 gagcatatgg acgatgccgg tttcgccctg ggtccggacc agcatgcgag tcggttcggc
    17041 atctcccagg tgctgcaggt tgtgctggat gctgcccaga acctgcgagc tgctctgcat
    17101 ttcggtctcg aggcgctcga tcagcgcgtt gcgctcatcc agcgagctgt tcacggcggc
    17161 gagctgcgcc cgcagttccg ccatcgcggc ctgctgcgat tccagctcgg cgcgcacgct
    17221 ggcgacgcgt tcgtcggcgg ctcgagccgc ctcggtcagc gagaggcgca gtgattcctg
    17281 ctccgtgcgg ctgatgatcg cagcctgctc gagctggcgt acccgctcct gctctgcgct
    17341 gagcagcccc ttcaggtcgc tctcgcgggc agcgctgaac cgggtgcgct gggtcatctc
    17401 gctttcgagc ttgaccaggg tagtgccgcg ttcgatgact tctgcctcgc gctcatccag
    17461 catgctcgcg aagatctgac gccggccttc catgctctgc aggcgttcgc ggtagcgctc
    17521 tgcgttggcg cgcgcctgcg cgatgtcggt ctcgagctga cggactcgac gattttgttg
    17581 ctcgcgctcc ttcaatagcg cgccttcgga ggcgtcgcgc gctttgatca gctggcgaga
    17641 agaatccgca tcttcgcgcg ccaccctcag gtccaagcgc agcgtgtcgc gctccgcggt
    17701 ggtggccgcc agcgcgccgt caagcgtcgc cgtacgggct tcgagctcgg agattcgctg
    17761 gtgtgaggat tcgatgtcag cgcgtaccgc ctgcagttcg acgcgcagtg aatcccgctc
    17821 aacgcgcagg gaatcgcgct ccgtgatttt ggcagccagt tcgccatcga gggtggcgtt
    17881 gcgagctgcc agttccgaga tgcgtcgatc acgctgtcca acctcgcgct cgaacatcga
    17941 gagctgctcg ctcttggtgt tgagcatctg ctcaaggtcg cgcaggttgg tcgtcaggtt
    18001 gccgagattg ttttcgagct gtccgcgact ttcggttacc gaggccagat cggaacgcag
    18061 cgccccgatc tcggcctcac gctgggcaat acgcgcctcg cggatgtcat cgatcgccgg
    18121 gggcgcacga aataccgtgg gtgcgttgta ggcatcggtc gccaccaacg gatcctcgga
    18181 caccgaaacg ccagcgcctc cggggatgat cggcagctca gctgtctcgt cgaggtcggc
    18241 cgcggtcggg gtctcttttt gcttcgccat caactcgtcc tacgccagtc ccagaaaatc
    18301 ccgcaactgc gccgcttggc gctccagctc cgcggcattg tggccgcccg cgtccgccgg
    18361 acgcaggtca atcaccagct gcggctgccg tagagagcgc gcatcggcgc gaaccgcatc
    18421 cgtgttcggt gaaagtacca cgcggccgac ctgagccgaa cgttgcgagg ccagtgccag
    18481 agcgatattc acaccgccct cgtgcgctag caaatcaacc tgcctcaaat gcatccaatc
    18541 cagaaaatcg ccgagcgctg cggccgcgct ggtggcatcc agcatacctg gggccggatc
    18601 ggactccccg ctgcccggca agtcaggcgc gtaaaccgat cgatccctcc ccaggggcag
    18661 cagcaagggt cgaaagaatc gtccaagccc cggtgcaccc ggaatgcaca gcaaggtggt
    18721 cgcttcgtcg aagccgccac cggctggaat cgcatagtgc acgtgcagct ggccgaaacg
    18781 acaatcaaag tacgcgcgcc gcatgcgcgg ttccgtcgta gcggcgacac gaacatctcg
    18841 tcgggctcta atcatgcaac caagggtcct ttgagtcttt ttgcgccgct cccgccacga
    18901 cgcttcctcc gccagaaagg tcattattcc tcaaggttcg gtgccgccct gcgacacact
    18961 acccaacttc ggctaactct tcctgacagg atgctcaccg gaaagggttg cgttggacat
    19021 aaccgtattg actttctaga gcgggccatc gaccaaaatg gccataaaca ccgcgccgcg
    19081 ggcactccat tggggcccgg gccggttgac gccccgcagt acgcggtgtt cggatggccg
    19141 cgatgccttt gctgcgcccg ttagtgctcg caagcgctct cctgggagcg gcgctgacag
    19201 tgagcgcggg aaacgcggaa agcaccgatg cggggctgcc cccggatgcc gagctggaag
    19261 ccgctcatgc gcacatcggc aagatcgagg tgcggaccct gcagatcttt gacctcggtg
    19321 acccggcaga caattactgg ctgttccgca cggccgatca cctgcatcag cgcacccgca
    19381 attccgtgat cctggcgcag ctactgttcc gcagcggcga tccttactcc cgccggatcc
    19441 tggatgagag cgcgcgcaat attcgtttga attcctcgtt cttgcgggag ccggtgattc
    19501 atccgatccg ctaccacgat gggctggtgg atatcgaagt gatcacccac gacgtgtgga
    19561 cgtttgagcc cggcgtgaac ttcagtcgca gcggcggcgc caatacgacc ggcgtcgaca
    19621 tcgccgatgc gaacttcttt ggcttcggca agtacatcga aatcggtcac ggccagaacg
    19681 ttgaccgttc ttccaatttc ctgcgctggc aggacccgaa tgtctggggc agccattgga
    19741 ccgatggcgc ggtttattcg aacaactcgg atggaacggt ctggcagctg gtcggtggct
    19801 taccgtttta ctcactggaa tcgcacgccg ccgcgggcgt ggatctcctc gacaaccaca
    19861 gcatcgtcac gcgctatcgt ctgggccgga cctacgacgg ctatgacgtc aacgaccatg
    19921 ccggtgacct gttcgtcggc cgtgcaatcg tggtcaccga tgcctggacc gaacggatgc
    19981 tgctcggctg gcgtgtggat aacgacaatt tcagcgcacc gccgggacag accccactgg
    20041 ctgccttacc gcaagaccgc gacctgtctt accccttcgt tcggatgcag tgggtcgaga
    20101 atcgcttcga cacgatgcgc aacctcgacc tcatcgcgcg caccgaggat gtgcacttcg
    20161 ggctcgatgc gagcatcgga ctgggtttgg ccacgcccgc cctcggcgcg gatcgacatt
    20221 cagtgcttgc agatactgag atcaactatg gctggcgatt cagtgaaacc gaggaaatgt
    20281 tcctgaccag tcgcttggcg tcccgcttcg aggacggtgg tctgcgcgac gccatgatca
    20341 cttggggtgc cgcctactac ctgcagacca gcgagatgac ccgcatgctg gtgcgtttca
    20401 cgggtgatgc cggccacgat ctggatggcg atcacaccgt gcagctcggc ggcgataccg
    20461 gactgcgcgg ctaccccctg cgttatcaga acggcaatca gcgcgcactg ttcacgctcg
    20521 aggagcgcct gtacacgaac tggtacctgt tccggctgtt caacgtcggt gc
//
LOCUS       DATMRM010000093         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4395835, whole genome shotgun sequence.
ACCESSION   DATMRM010000093 DATMRM010000000
VERSION     DATMRM010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4395835"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VNZ06_02820"
     CDS             complement(<1..323)
                     /locus_tag="VNZ06_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19714.1"
                     /translation="MHGRLMSGSVQLAVRDVELAVGVDSNRDGRVTWRELRAAAPAVR
                     SYLGDHVTVEAAGARCPVQFGELQVNPRVDGNYAWIAVSVQCPTEPVQITIRYQVLNG
                     IDPSHR"
     gene            complement(416..1588)
                     /locus_tag="VNZ06_02825"
     CDS             complement(416..1588)
                     /locus_tag="VNZ06_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWW19715.1"
                     /translation="MTFGLMRSGQPRVTRGWLAAALLGIATFSAVASPYTPAHEDEVL
                     ASLPAGGVHVTLPARQQAAARLDVALPLAQFYIEQGRKNGDLRFLGYAEATLAPWRDR
                     NPEIPAVLVLQATILQSRHEFDAALNNLARALTMRPDDPQGWLLRATVLRVLGRYPEA
                     VASCARITPADPAVASICLQSTRSLHGQLQLAYAALRALTPDALSTEARAWRASELGE
                     MAVRLGEDAAAERWFAQALRLAPGDAYTRAAYADLLLSERRAAETLALLKDYESMEPL
                     LLRIAIAQQQLGDPDAARSRAQLAEAFSVEQQRGESVHRREQARFLLDVVHDPAAAVE
                     AASANWQVQREPADILILLRTAEAAHNPAAAAPALQFAHRESTEDARFAPYLRGAP"
     gene            complement(1589..1846)
                     /locus_tag="VNZ06_02830"
     CDS             complement(1589..1846)
                     /locus_tag="VNZ06_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19716.1"
                     /translation="MKLRIAMIATTAAGVAALSGCGGSEESAAMSPPLTTPPGQGLVT
                     SQVLAQARQSSETAEPYAVNDGVVSLLDSSETTDPINIDVT"
     gene            complement(1858..>2057)
                     /locus_tag="VNZ06_02835"
     CDS             complement(1858..>2057)
                     /locus_tag="VNZ06_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19717.1"
                     /translation="DIELRVAMGVLLSPFDGSASDPDPASDVSRQLHYTDGAEPNPAD
                     YLPVFPYLNTPLAGSPTSAND"
BASE COUNT          327 a          685 c          710 g          335 t
ORIGIN      
        1 cggtggctgg ggtcgatgcc gttcaagacc tggtagcgga tcgtgatctg caccggctca
       61 gtcggacact gcacgctcac ggcgatccag gcgtagtttc cgtccacgcg cggattcacc
      121 tgaagctcgc cgaactgtac cgggcagcgc gctccggccg cctcaaccgt tacgtggtcg
      181 cccaggtagc tgcgtaccgc cggcgcggcc gcgcgcagct cgcgccaggt cacgcgaccg
      241 tcccggttgc tgtcgacgcc gacggcaagc tcgacgtcgc gcactgcgag ctgcaccgag
      301 cctgacatga gacggccgtg catggtgagc tcaaggaagc tgttactggc cacatgcgct
      361 cgcgcacctg cgctcaaaag caggagcaga gcggccgcga cgagtgcgcg tgcagtcaag
      421 gtgcgccccg caggtagggg gcgaagcgcg catcctccgt ggactcgcgg tgtgcgaact
      481 gtagcgccgg tgctgcggca gcaggattgt gcgcggcctc tgcggtacgc agcaggatca
      541 gaatgtcagc cggctcgcgc tgcacctgcc agttggcact cgcggcctcg acagccgccg
      601 ctggatcgtg cacgacgtcc agaagaaacc gcgcctgctc gcgccggtgt acggactccc
      661 cacgctgttg ctcgacgctg aaggcctcgg caagctgcgc acggctgcgg gcggcgtcgg
      721 ggtccccgag ctgctgctga gcgatcgcga tccgcaacag cagaggttcc atcgattcgt
      781 agtctttcag gagcgcaagc gtctctgcgg cacgccgttc ggacagcagc agatccgcat
      841 atgccgcgcg cgtgtaggcg tcccccggcg ccagtcgcag cgcctgcgcg aaccaacgct
      901 ccgccgctgc gtcctcgccc aggcgaactg ccatctcgcc aagttcggaa gcacgccagg
      961 cgcgtgcctc ggtggatagc gcgtcggggg ttagtgcacg caatgccgcg taggccagct
     1021 gcagttgtcc atgtagcgag cgcgtactct gcaggcagat actggccacc gccgggtcgg
     1081 ccggggttat ccgggcgcag gaagccaccg cttccgggta tcggcccagg acgcgcagga
     1141 ccgtggcccg caggagccac ccctgggggt catcggggcg catcgtgagg gcacgcgcca
     1201 gattgttgag cgccgcgtcg aactcgtgcc ggctctgcag gatcgtagcc tgcagcacca
     1261 gcacggccgg aatttcgggg ttgcggtctc gccacggggc gagcgtggcc tctgcgtagc
     1321 cgaggaaccg caagtcgccg ttctttcttc cctgctcgat gtagaactgc gcgagcggca
     1381 gggcgacgtc caggcgtgcg gccgcctgct gccgcgccgg cagtgtgacg tgcacgccgc
     1441 cggccggcag actcgccagc acctcatcct cgtgggccgg agtgtaggga ctcgccacgg
     1501 cgctgaatgt cgcaatccca agaagcgccg ctgccagcca gccccgggtg acccggggtt
     1561 ggccggagcg catgaggccg aacgtcatct aggtgacgtc tatgttgatc gggtctgtgg
     1621 tctcactgct gtccagcagc gagaccaccc cgtcgttgac tgcgtagggt tcggcggtct
     1681 ccgaagactg ccgcgcctgc gccagcacct gcgaggtcac cagaccttgc ccgggtggag
     1741 tggtgagcgg cggcgacatg gccgcagact cctcgctccc gccgcagccg gatagagccg
     1801 ccactcccgc tgctgtcgtt gcgatcatcg caatcctgag tttcatggat gctccgttca
     1861 gtcgttggcg ctggtgggtg aaccggcgag cggcgtgttc agatacggga aaacgggcag
     1921 atagtccgcc gggttgggct cagcaccgtc ggtgtagtgc agctggcgag atacgtcgga
     1981 agcagggtcc ggatcactcg cactgccgtc gaagggactg aggagcaccc ccatggccac
     2041 gcgcaactcg atgtcca
//
LOCUS       DATMRM010000094         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1606422, whole genome shotgun sequence.
ACCESSION   DATMRM010000094 DATMRM010000000
VERSION     DATMRM010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606422"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /locus_tag="VNZ06_02840"
     CDS             <1..1548
                     /locus_tag="VNZ06_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWW19718.1"
                     /translation="PIFRVTVVERTTNAVSYRHRSGWTKVDLHGTPLAPDATGHADVN
                     SRPGYIEAKAEMHRLLPASQYGPEYMTYVLWAITPEGRAKNLGEVVLDNGGSHLDVTT
                     DLQAFALIVTAEPYFGVTQPSDVVVMENIIREDTIGKIEQMSVKYDLLKRGTYTMHAQ
                     PGRYHQVKTDKRIPLQLLEAENAVQIAQLVNADQYAKDTYDNAQQLVKQAEAYQARNA
                     GSKPVIMTSREAVQAAETSRLLALKRQEDERIAQEKKEAADREAAARAQAEEQTRQRQ
                     LADAQAAESARQKALADAQAQAASQRAAADRATADAARAQAEAERLRAERERATAEDQ
                     QRQAELARLQAQQAQQQAQLADQARLQAEQAQQQLRQQLLQQFNVILETRDTARGLIV
                     NMSDVLFDFNKYTLRPAAREKLAKISGIILSHPGLRLEVDGYTDSIGSEAYNQKLSEE
                     RADGVRTYLVSEGLSSDIVISKGFGKDNPVASNDNAAGRQKNRRVEMVVSGDIIGQPI
                     GGIRSSR"
     gene            complement(1612..>2010)
                     /locus_tag="VNZ06_02845"
     CDS             complement(1612..>2010)
                     /locus_tag="VNZ06_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19719.1"
                     /translation="GIFTAGPPTFYAIAQNVDGTLAQAAGSIPGLTTHPAKAGDTIVI
                     YATGLGPVDNTPADGGIPSTLARTLTMPIVLVGGMQADVAFSGLQPQFVGVNQVNIVI
                     PNVPPGNAVPLQIQMGGVTSPNNVTIAVSQ"
BASE COUNT          457 a          633 c          594 g          326 t
ORIGIN      
        1 cctatatttc gcgtgaccgt ggtggagcgt accaccaatg ccgtgagcta tcggcatcgc
       61 agcggttgga ccaaggtaga cctccatgga accccactgg cgcccgacgc gaccggccac
      121 gccgacgtca atagccgccc aggctacatc gaagcgaagg ccgagatgca ccgcctgctc
      181 ccggcctcgc agtatggtcc cgaatacatg acctacgtgc tgtgggccat tacccctgaa
      241 ggacgcgcca agaatctggg cgaagtggtg ctcgacaacg gcggttccca tctcgacgtc
      301 actactgatc tgcaagcctt cgcgctcatc gttaccgcgg agccctactt cggcgtcacc
      361 cagcccagcg atgtggtggt catggagaac atcatccgcg aggacaccat cggcaagatc
      421 gagcagatga gcgtcaagta tgacctgttg aaacgcggca cctacaccat gcacgcccag
      481 ccgggccggt accatcaggt gaagaccgac aagagaattc cgctgcagct cctcgaagcc
      541 gagaacgcag tccagatcgc ccagttggtg aatgccgatc aatacgcaaa agatacctac
      601 gacaacgccc agcaattggt gaaacaggcc gaagcctatc aggcgcgcaa tgccggctct
      661 aagccggtca tcatgacttc gcgtgaggcg gtgcaggcag ccgagacgtc ccggttgctg
      721 gcgctgaagc gacaggaaga cgagagaatc gcacaggaga aaaaagaagc cgcggatcgt
      781 gaagccgccg cgcgcgcgca agccgaggaa cagactcgcc agagacagtt ggccgacgct
      841 caggccgccg agtcagcccg ccagaaggcg ctagccgatg cgcaagcgca ggctgcctcc
      901 cagcgtgccg ccgccgacag agccaccgcc gacgcagctc gtgcccaagc agaagccgag
      961 cgtctgcgcg ctgaacgtga gcgcgccacc gccgaagatc aacagcgcca ggccgaactc
     1021 gctcgtctgc aggctcaaca agcgcaacag caggcccaac tggccgacca ggctcgttta
     1081 caggccgagc aggcacagca gcagctgcgg cagcaattgc tgcagcaatt caacgtaatc
     1141 ctggaaactc gcgataccgc gcgcgggctg attgtcaaca tgtccgacgt tctgttcgat
     1201 ttcaacaaat acaccctgcg gccggccgcg cgcgagaaac tggccaagat ttcggggatc
     1261 attctttcgc atccgggctt gcggttagaa gtcgatggct acaccgacag catcggcagc
     1321 gaggcttaca atcaaaaact gtccgaagag cgcgccgacg gcgtgcgaac ctacctggtc
     1381 agtgagggcc tatcctctga catcgtcatc tccaagggtt tcgggaaaga taacccggta
     1441 gcctcgaacg acaatgccgc cggccggcag aagaatcgcc gcgtggagat ggtcgtatcg
     1501 ggcgacataa tcggccagcc catcggaggc attcgctcca gccggtaggt aactgtctgg
     1561 gggtgcgcag gagtgcgcac ccccagcacg catgagtgcg tgcgctatac gctactggct
     1621 cacagcaatc gtaacgttgt tcggactggt gactccgccc atctggatct ggagtggcac
     1681 cgcgtttcca ggtggaacat tgggaatcac gatattcacc tgattcacac ccacgaactg
     1741 cggctgcaaa ccggagaatg caacgtccgc ctgcatgccg cccaccagca cgatcggcat
     1801 agttaatgtg cgggcaagtg tggacggaat ccctccgtcg gccggagtat tatccaccgg
     1861 ccccagcccg gtggcataaa tcacgatcgt gtcgccagct ttcgcagggt gtgtcgtcag
     1921 gcccggaatg ctgcccgctg cttgagcgag cgttccatcg acgttctgtg cgatggcgta
     1981 aaaagtcggc gggccagcag tgaaaatccc
//
LOCUS       DATMRM010000095        13006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4396352, whole genome shotgun sequence.
ACCESSION   DATMRM010000095 DATMRM010000000
VERSION     DATMRM010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13006
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4396352"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..800
                     /locus_tag="VNZ06_02850"
     CDS             207..800
                     /locus_tag="VNZ06_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19720.1"
                     /translation="MHITCEPAILYLGTPVVLISAVNEDGSYNLAPMSSAFWLGWRCM
                     LGLSVFSQTTHNLMRARVLECPIQLEAIIEATHPLAQTDEKLRGRIIVFEARVQRVHI
                     EESLLKTGEPNRIDPDRWRPLIVSFQRFYGLTARQLHESTLAKIPESAYRSPDVDQSR
                     LATLGQLATAAPGSETFSQELIAGARHESQSFVECTS"
     gene            complement(726..1178)
                     /locus_tag="VNZ06_02855"
     CDS             complement(726..1178)
                     /locus_tag="VNZ06_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWW19721.1"
                     /translation="MSPTIRAARPSDCEELVSLVERYWNFERIEGFDREAIAKLLRGF
                     IAQPERSRCWLAGDPGEVRGYLLAVYVVSLEFGGTVAEIDELYVLEAYRSMGLGERLV
                     QQAMADMKQAGVVHLQLQLGTANASGRAFYERLGFVARTGYELLGKSL"
     gene            1250..1870
                     /locus_tag="VNZ06_02860"
     CDS             1250..1870
                     /locus_tag="VNZ06_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19722.1"
                     /translation="MFARIMGAMVATRSAALGVAIHSGWAAAVALGNSASDPHILLRS
                     RIEMSDAGVPDSKQPYHAVESLELEHARQRLEAFAASAQTKARAAIEQLRQSAAQRGY
                     QLGSLGILDSSGRSGDTLAAILASHTLIHTADGNHFRRAIASAAHDCGLQVRRLAAKG
                     LDAHAQARLSLSQEAIEGTLTSMGRQIGAPWGADQKKAALLAWTLL"
     gene            1960..2451
                     /locus_tag="VNZ06_02865"
     CDS             1960..2451
                     /locus_tag="VNZ06_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWW19723.1"
                     /translation="MIEDIVRQLGYLTLGSRLKRLGERLQSQAQALLEEAGPAISASY
                     FPLLAALDRLGALTVGELSQALGITQPGVTRLLDKLESEDLVNSQATTGDRRVRSVVL
                     SPAGRQMVERSKKLAWSRIEAAVADACAGPKRPLLAQLAALEDALTAVPLRERVTRVS
                     TGE"
     gene            2455..3144
                     /locus_tag="VNZ06_02870"
     CDS             2455..3144
                     /locus_tag="VNZ06_02870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWW19724.1"
                     /translation="MPALDRPVWASLSTHHSSLSVGNALAKRFLSDVNLFASSRDNSP
                     QSLAALSALVSRGERVYVLQAPDIIVPAGLSAVRRAKGVQMVAGRSLSGEPRRTDIVT
                     LTDADAPDMLALATLTEPGPFLTRTHRMGTFRGIRVAGRLAAMAGERFRFPGYTEVSG
                     VCTHPDFRGRGLARCLSAVVATHIESRGERPFLHAWKSNQAAISLYQSLGFELRAEVD
                     VAVLERMDTND"
     gene            complement(3134..6151)
                     /locus_tag="VNZ06_02875"
     CDS             complement(3134..6151)
                     /locus_tag="VNZ06_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWW19725.1"
                     /translation="MKLSVDEWQAASELLDEALELPAAARLAWVETLSGDRSALKPLL
                     RELLARQGRLETEDFLGAAPPMVPLLPARARGPIDSLLAGTTVGPYRLVRELGRGGMG
                     TVWLAERADALIRRPVALKLPHATLYDDHFAQRFARERDILAALNHRSIARLYDAGIS
                     AVGQPFLALEYVEGTALTEYCDQRAMPLDARIGLFCEVLHAVDYAHGLRVIHRDLKPS
                     NILITTSGEVRLLDFGIAKLIPESGGQETELTRIGGRAFTIDYAAPEQLARQPVSAES
                     DVYSLGVILSEILTGSRPYRPQRQSLGALEEAILTEEPLRPSAAELSEDTAQRRAVSP
                     ARLRKQLRGDLDNIVLKALRKRPSERYGSAESFALDLQRYLRGQPVEARADTALYRLG
                     KFAARNKLLVGAASVAMGALIVGAALSLWQARVARAQAAAALQEARRAQVVQNFLLDI
                     FRANSHLQSDPQKARQTTARELLDTGARRVGEDLKDVPAAEEQVLGTLSDMYEQLGLQ
                     QEAAALQRERVSAVKRAYGTQDLRVASALSSYAKTLADTPDRSRIASVLGEAKKILDD
                     GHDDSSIVRASVLVDYASLYRYSSPQESRRFADEDVSLLEKYHPDDGMLLGALQFAAR
                     ARSALGQYQAAVVLYQRELGAIAHQVGSGTAWDVSPLAQLAGAQAELLQFTDAERSFR
                     SSLAVSERLNGETHNETLQTQIRLAAFLNATSRRVEGERLMQRAWSVFSADPSKKDNA
                     VASVMYGLYGQGLMDAGELQAAQPLIDAELEEVRSLYPESTLLSRALLRQATLRIELG
                     QYDAAEAEVKQALVGWRHLGGTEADPALENPYLLALARIDLARVRPADAQRWLQQIHH
                     PAFAAALPFEPDEVRRQVLLAQVRLLEGRAGEAMTSAGAALAIVQRSAMRAYFQRLEA
                     ESAWTLAVAERRSGLAEDARSHLQGALDLLRINDDARSLSIARAQLTLAACLNDVNEP
                     QAARKLLDEADSTVASHRELSAEASRVLRNLSR"
     gene            complement(6245..6805)
                     /locus_tag="VNZ06_02880"
     CDS             complement(6245..6805)
                     /locus_tag="VNZ06_02880"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HWW19726.1"
                     /translation="MADEVWAPLLQEAESGVPGGKERLFTALYAELHRLAERQLRASS
                     GVPISPTTLLHETYLGISRGQAVFTNRGHFMSYAARVMRSLIIDFARARRAQKRGAEF
                     HITQFETGAAEQQSASVDDRMLEQLSLALDELAVHDPRLAEVVDLRYFCGLSVAEVAA
                     MRSVSERTVQRDWDKARLFLFHELED"
     gene            complement(6933..7544)
                     /locus_tag="VNZ06_02885"
     CDS             complement(6933..7544)
                     /locus_tag="VNZ06_02885"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWW19727.1"
                     /translation="MGCHAVASAAIGVVRPCGGTQATLANRSLSVHSARPQGARVGCA
                     ISVGTTMALEPSWRAVQVHQGSCLCGSIRYRIESDLKAVVNCHCRFCRKAHGSPFTTL
                     LFLPFSSLKILQGAEVLVRYRVERLNAERCFCSKCGTRLYNHMPSLGTASVVVATLDI
                     DQTLRPVAHLNTESKCSWHQITDQLPQFLSAPGPKQFTQLLTN"
     gene            7533..8939
                     /locus_tag="VNZ06_02890"
     CDS             7533..8939
                     /locus_tag="VNZ06_02890"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HWW19728.1"
                     /translation="MTTHRQSNPGESTERQRLSLRNLSHRAMRISWWDLTQTLGPILL
                     LGVLGVLLALHFGGPAPPHSISIASGPEGSNFATMAARYQKVLKRNGIELKVIDTEGS
                     LDNLKRMADPKSRTDIALVQSGLAEGGDNSDLDSLGSVFYQALTIFYRSPKPLMRLSE
                     LRGGRIAIGPEGSGTRALALALLKANEIEPGGSTLLTSDEGEGAREALLKREVDAIFL
                     TGDSASGTTIREMLHTEGVRLFDFEQADAYTRRFPYLNKLVVPAGAFDLGEDLPASDV
                     NLLAPTVELVAHPNLHPALVDLLIEASEEVNGRASLMQSAGQFPTPALHTYPISDEAM
                     RYYKSGNKSFAYRYLPFWLASLFNRLIVVLVPLFVVVVPGLRFLPALYRWRIATRIHR
                     RYGELMAVEREALGPLSPERRLTLLERLEEIERSVISHRMPGSHAEQVYVLREHIRFV
                     RDNLARPIVTPLEPVAGG"
     gene            8970..10118
                     /locus_tag="VNZ06_02895"
     CDS             8970..10118
                     /locus_tag="VNZ06_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19729.1"
                     /translation="MCLARAEKRPRSTFFAVAFTGWLTALACLGGCSLITIKSPEKPL
                     STRDLNARILTHEYAAHFISAIEQTADEIADGTQDTRIRLNTLRWKIAVAASSQRAAS
                     QIVPMMSLLDTWALSVQMAQYLDTGNGGALFGAQQSQAVTLARELAHDAETSARGLLS
                     SQEFTRDQRFVEHYARDYPLQSLNFTRPSIVERWVRENGSESKLIESLGTVPESLAEA
                     RDLVRMYGDNTPEQILWKAQLLAQQSGVNGRDVQSALKQLDERMARLSALADQTPDLL
                     NGIVRDVQRRADTSWSQMLGELRTQGMTLSGTLSSEREAATQSINEQREALTADAVRI
                     STQLVQQAGIEARRLVREALILIIVLALVVLGLPFTAGYLVGRARRSA"
     gene            10255..10965
                     /locus_tag="VNZ06_02900"
     CDS             10255..10965
                     /locus_tag="VNZ06_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HWW19730.1"
                     /translation="MKTIGLIGGMSWESTIPYYRIINETVRERLGGLHCARVILFSVD
                     FAEIERLQHQGDWEQAGRSLALAARSLERAGADFMVLCTNTMHKVAAAIESAVSIPLL
                     HIADAVATTVTAAGHRSVGLLGTRFTMEQPFYKERLHSRFGLSVLVPPANDQSRIHRI
                     IYDELCLGKVLPQSRAEFQRVMDDLVSRGADAMILGCTEISLLVGSEDASVPLFDTTR
                     IHAQMAAAMALQTSHQTT"
     gene            complement(11621..12394)
                     /locus_tag="VNZ06_02905"
     CDS             complement(11621..12394)
                     /locus_tag="VNZ06_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19731.1"
                     /translation="MWRGLRISVLLLVLLVVAGQAWLDRFSTTRWQRTVYVGAFPVGA
                     DASTATARYLAQLDQRKIDEVSDFLKAEARRYGVSADEPIELKLYPTLTVPPPELDSG
                     SGVFTRILWSLKLRYYRSRALAAVSRSRPKIALFLLYHDPELTQSLPHSAGLQRGLTA
                     VVHVFASRSQEAQNRIVITHELLHTFGATDKYDLATGLPQYPEGFAEPQLAPRYPQQL
                     AEIMAGRMPLSASEARLPDSLEDERVGPMTAREIGWVSH"
     gene            complement(12617..>13006)
                     /locus_tag="VNZ06_02910"
     CDS             complement(12617..>13006)
                     /locus_tag="VNZ06_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19732.1"
                     /translation="GWLWMDPMMGIVGALVISLWAYALVRDTGRILLDMTPDSALLGR
                     IRRRLEADGDRIADLHLWRLGPGHLGAILAIVTPRARDTSFYHSLLKSFPSLSHVTVE
                     VISPSLVAALPATQPSGASADPEPAHR"
BASE COUNT         2498 a         4105 c         3968 g         2435 t
ORIGIN      
        1 gtaatcgtgc gtttcaccgg acagggggtc gcccgcaccg gaacccaata ggggtaccgc
       61 aatgcaggcc tcgtggtcgc catagcgggt atcgagctga ccatggggcg tgaatcttac
      121 cgaacaattc ggtcgcaaga atcggatcgt cgtgcagcat cggccttgcg aaactgagtt
      181 caggtcagcg caaggagccc actgtcatgc acatcacctg cgaaccggcg atcctgtacc
      241 tcggcactcc ggtggttctg atcagcgccg tcaacgagga tggttcatac aatctcgcgc
      301 cgatgtcatc ggcgttctgg ctcggctggc gctgcatgct cgggctgtcc gtattctctc
      361 aaaccacgca caacctgatg cgagcgcgcg tgcttgagtg tccgattcag ctcgaggcga
      421 tcattgaggc gactcatccc ctggcgcaga ccgatgaaaa gctccgcggc cggatcatcg
      481 tcttcgaggc acgcgtgcag cgcgtccata tagaagagtc gctgctgaaa acgggggaac
      541 caaatcgcat cgatccggat aggtggcgtc ctctcatcgt gagctttcag cggttctatg
      601 gcctgacagc gcgccagctg cacgagtcca ccctggcgaa gattccagaa tcggcctatc
      661 gcagccccga cgtggaccag agtcgcctcg caaccctggg acaactggcg acggctgcgc
      721 cagggtcaga gactttttcc caggagctca tagccggtgc gcgccacgaa tcccaatcgt
      781 tcgtagaatg cacgtcctga ggcatttgcg gtacctagct gcaactgaag gtgaacgacg
      841 cccgcttgct tcatgtcggc catcgcctgc tgcaccaagc gctctcccaa gcccattgaa
      901 cgatacgcct cgaggacata cagctcgtcg atttccgcga ccgtcccacc gaattccagg
      961 ctgaccacat agacggcgag gagatagccg cgcacttccc caggatcacc ggcgagccaa
     1021 caacgactgc gttcgggctg cgcgatgaag ccgcgcagca atttggcgat ggcctcccga
     1081 tcgaacccct caatccgctc gaagttccag taccgctcga ccaatgagac gagttcttcg
     1141 cagtcggatg gcctggcagc gcgaatggtg gggctcatcg cggtaaccta gcgcggcgac
     1201 gctgcggcaa caacccacca aggcctatgt ttcgaagagg gcgggcgcgg tgttcgcgcg
     1261 tatcatgggc gccatggtgg ccacacgttc ggcagccctc ggcgtcgcga ttcactccgg
     1321 ctgggcggcg gctgtggcgc tcggaaacag tgcttccgat ccgcacatcc tgctgcgctc
     1381 gcggatcgaa atgagcgacg ctggcgtccc ggattcgaag caaccctatc acgcggtgga
     1441 gtcgctggag ctcgagcatg cccgtcagcg cctcgaagcg tttgccgcca gcgcgcaaac
     1501 caaggcgcgt gccgcaatcg agcagctgcg gcagtcggcc gctcaacggg gctatcagct
     1561 cggctcactg ggtattttgg attcttcggg ccgcagcggt gacacgttgg cggcaatcct
     1621 ggcctcgcac acgctcatac ataccgccga tggcaatcac tttcgtcgtg cgatcgcctc
     1681 tgccgctcat gactgtggac tgcaggtccg ccggctcgcg gcgaaagggc tcgatgcgca
     1741 cgcgcaagcg cggttgtcgc tttcccagga ggcgatcgag ggaacgctga catcgatggg
     1801 ccgtcagatc ggtgcaccct ggggtgccga ccagaaaaaa gcggcactgc tggcgtggac
     1861 cttgttgtag ccatttagca gcagatcacc gagggcgagc atgacgcttg cagttgtcta
     1921 taacatggta tataacatgt tatatattgt gaggctcttg tgatcgaaga catcgtccga
     1981 cagctgggat atctgacgct cggtagtcgc ctgaagcgtc tgggcgagcg tcttcaatcc
     2041 caggctcagg cactgctcga agaggctggc ccggctatct cggcgtccta cttccccttg
     2101 ctcgcagcac tggatcggct gggcgcactg accgtcggcg agctatctca ggcgctggga
     2161 atcactcaac ctggtgtgac gcgcctgctg gacaaactcg agtcggaaga cctggtgaat
     2221 tctcaagcca ctacgggcga tcgccgcgtt cggtcagtcg tgctgagtcc ggccggtcgg
     2281 cagatggtgg agcgatcaaa gaaactcgcc tggtcgcgaa ttgaggccgc ggtcgcggat
     2341 gcctgtgcag gtcccaagcg cccgctgctg gcgcagctcg cggcactcga agacgctctg
     2401 acggccgtgc cgctgcgcga acgggtgacc cgcgtgtcga ccggagaatg agcgatgcca
     2461 gcactggatc ggcccgtctg ggcgagcctg agtactcacc attcctcgct gtccgttggc
     2521 aatgcgttag cgaagcgatt cttgagcgac gtcaatctgt tcgcatcctc gcgcgacaac
     2581 agcccgcaga gcctggccgc cttgagcgca ttggtgtccc gcggcgagcg cgtgtacgtg
     2641 cttcaggctc cggacatcat cgtgccggcc ggcctgagcg cggttcggcg tgccaagggc
     2701 gttcagatgg ttgccgggcg tagcctgtca ggcgagccgc gacgcacgga catcgtgacc
     2761 ctcactgatg ccgatgcccc ggatatgctg gcgctggcta ccctcaccga acccggtcca
     2821 ttcctcacgc gtactcatcg gatgggcaca ttccgtggta tccgggtcgc cggacgactg
     2881 gcagccatgg cgggcgagcg ttttcgcttt ccgggttaca ccgaggtcag cggcgtctgc
     2941 acccatcctg attttcgtgg ccgcgggctg gcccgctgcc tttcggccgt cgtcgccact
     3001 cacatcgaat cgcgtggtga acggcccttc ctgcacgctt ggaaatccaa tcaggccgcg
     3061 atatcactct accagtcgct gggcttcgag ttgcgagctg aggtcgatgt cgcagtgctc
     3121 gaacggatgg atactaacga ctgagattac gcagcacccg cgaagcctcg gcgctcagtt
     3181 cgcggtgcga agccactgtg ctatcagctt catcgagcaa cttgcgcgcg gcctgtggct
     3241 cattgacgtc gttcaagcac gccgccagtg tcaactgcgc tctcgcaatg ctgagactgc
     3301 gcgcatcatc gttgatgcgc agcaaatcaa gcgctccctg cagatgcgag cgtgcgtctt
     3361 cggctagtcc gctgcgccgc tccgcgaccg cgagcgtcca ggctgactcc gcctcgaggc
     3421 gctgaaaata cgcccgcatc gcagaccgct gcacgattgc caatgccgcc ccggctgacg
     3481 tcatggcttc cccagcgcgc ccttctaaca agcggacctg tgcaagcaag acttgtcttc
     3541 gcacctcatc cggctcgaac gggagcgccg ccgcaaacgc gggatgatga atctgttgca
     3601 accaacgctg cgcatcggcc ggccgtacgc gcgccaggtc aattctggcc aatgccagca
     3661 gatacggatt ctcgagcgcg gggtctgcct ccgttccacc caggtgccgc cagccgacga
     3721 gtgcctgttt cacttcggcc tcagccgcat cgtattgtcc cagctcgatt cgtaaggttg
     3781 cctgtcgcag caatgcccgc gacaggagtg tcgactcagg atacaggctg cgcacttcct
     3841 caagctctgc gtcgatcaac ggctgtgctg cctgcaactc gccggcatcc atcaggccct
     3901 gtccatagag cccatacatg acactggcga ccgcgttgtc cttcttgctg ggatcggcac
     3961 tgaacacgct ccaggcgcgc tgcatcaacc tctcgccttc cacacgccgg gacgtcgcat
     4021 tcagaaatgc ggccagacga atctgggtct gcaacgtctc attgtgggtt tcaccattca
     4081 gccgctcgga caccgcgagc gaagaacgga agctgcgctc ggcatcggtg aattgcagca
     4141 gttccgcctg agcgcccgca agctgcgcca gtggagagac atcccacgcc gtgccagagc
     4201 cgacctgatg cgcgatggca ccgagctctc gctgatacag cactaccgcc gcctgatact
     4261 gccccagcgc cgagcgggcg cgggccgcga actgcaacgc ccccagcagc atgccatcgt
     4321 ccggatgata tttctccagc aaagagacat cctcgtcagc gaaacgccgc gactcctgcg
     4381 gcgagctgta gcggtacaaa ctggcgtagt ccacgagcac gctggcgcgc acgatcgaag
     4441 agtcatcatg cccatcatcc agaatcttct tcgcctcacc gagcacggag gcaatgcggc
     4501 tgcgatccgg cgtgtccgcc agtgtcttgg cgtagctgga gagtgcgctg gcgacacgca
     4561 ggtcctgcgt tccgtacgcg cgtttcacag cgctcacccg ttcccgttgc agggccgccg
     4621 cctcttgctg cagaccgagt tgctcataca tgtcggaaag agttcccaag acctgttctt
     4681 cggcggcggg cacatccttg aggtcctcgc ccacgcggcg tgcgccggta tccagcagct
     4741 cacgtgcggt cgtttgccgc gctttctgcg gatccgattg cagatgcgag ttggctcgaa
     4801 aaatgtccag taaaaaattc tgtacgacct gcgcgcgcct tgcttcctgc agtgccgctg
     4861 cggcctgcgc acgtgccacc cgcgcctgcc agagcgacag cgctgccccg acaatcagtg
     4921 ctcccatcgc aaccgaggca gctcccacga gcagcttgtt gcgagccgca aatttaccga
     4981 gccgatagag ggcggtgtca gcgcgcgcct cgaccggctg accgcgaaga tagcgctgca
     5041 gatcaagcgc aaagctttca gcagatccat agcgctcgga cggacgcttt ctgagagctt
     5101 tgaggacaat gttatccaga tcgccacgca gctgtttccg cagtcgagct gggctcacgg
     5161 cccggcgctg cgcagtgtct tcggaaagct ccgcggcgct gggcctcaac ggctcctcag
     5221 tgaggatcgc ctcttcgagc gcccccagcg attggcgctg tggccgatag ggacgcgagc
     5281 cggtgagaat ttcagacagg atcacgccca gagaatagac gtcggactcg gcgctcaccg
     5341 gttgcctcgc gagctgttcg ggagcggcgt agtcgatcgt gaacgcgcgc ccgccgatgc
     5401 gcgttagctc ggtctcctgg ccaccgcttt cgggaatcaa cttcgcgata ccgaagtcca
     5461 gtagccgcac ctcgccggag gtggtaatca gaatgttgga aggcttcaga tcgcgatgga
     5521 tgacgcgcag cccatgggcg tagtccaccg catgcagcac ctcgcagaac agcccaatcc
     5581 gcgcgtccag cggcatggct cgctggtcgc aatattcggt gagtgcggta ccttcgacat
     5641 actcgagtgc aaggaacggc tgaccgactg ctgaaatgcc ggcgtcgtac aggcgcgcaa
     5701 tactgcgatg attcagagcg gcgagaatgt cgcgttcgcg ggcgaaccgc tgcgcgaagt
     5761 gatcatcgta aagcgtcgcg tgtggcagct taagggcgac cggacgccga atcagggcat
     5821 cggctcgctc cgccagccac actgtcccca tcccaccccg gcccagttcc cgcaccagcc
     5881 gatacgggcc gacggtcgtg ccggcgagga gtgagtcgat tggaccccgc gcgcgcgcgg
     5941 gcagcagagg caccataggg ggtgcggcgc ccagaaagtc ctcggtctca agccgtccct
     6001 gccgcgcgag cagttcacgc agcagcggct tgagcgcact gcggtcgccc gatagggtct
     6061 caacccacgc cagacgcgca gcggcgggca gctccagcgc ttcatcgagc agctctgacg
     6121 cggcctgcca ttcgtcaacc gaaagcttca ccccaccctt ccttgtcagg cgttcaacgc
     6181 ctccatccga cagtccacac accggggaag cggattcggg gcgcccgccc tgacagcgct
     6241 cgctctagtc ctcgagttcg tgaaacagaa acagccgtgc cttgtcccag tcacgctgta
     6301 ccgtccgctc ggagacgctg cgcatcgcgg ctacttcggc gaccgacagt ccacagaagt
     6361 agcgcagatc cacgacttcg gccagacgcg ggtcgtgcac cgcgagctca tcgagcgcca
     6421 acgacagctg ttcgagcatg cggtcatcaa cgctggcgct ttgttgctca gcggctccgg
     6481 tctcgaattg cgtgatgtgg aactcggctc cccgcttctg cgcacgtcgt gcccgcgcga
     6541 aatcgatgat cagactgcgc atgacacgcg ccgcataact cataaagtgg ccacggttgg
     6601 taaacacggc ctgaccgcgc gaaatgccca ggtaggtctc atgcagcagc gttgtaggac
     6661 tgatcggcac tcctgaactg gcccgcagct gccgttcggc cagccggtgc aattcagcat
     6721 aaagcgcagt gaacagacgc tcctttcccc cggggacgcc cgattctgcc tcctgcagca
     6781 gcggtgccca gacctcgtcc gccatgtcgc tgtggtagct cgccgccgaa acctgagctg
     6841 cagggtatcg gttgcgtcca gagcggacaa gcggcaggcc ctatgtcaag tcatgtggct
     6901 gcctgagcgc cttggctggt accgctgcgc ggtcagttcg tcagcaactg ggtgaactgc
     6961 ttcggaccgg gcgccgacag gaattgcggc agctggtcgg tgatctgatg ccaggaacac
     7021 tttgactcgg tgttgagatg ggccacgggg cgcaatgtct gatcgatgtc gagcgttgcg
     7081 acgacgacgc tagccgtacc cagcgaaggc atatggttgt acaagcgcgt cccgcatttg
     7141 ctgcaaaagc acctctccgc attcaggcgc tccacgcgat acctgacaag aacttccgca
     7201 ccctgcagga ttttcaaact ggagaacggc agaaacagga gcgtcgtaaa cggtgaaccg
     7261 tgcgctttgc gacagaatct gcaatgacag ttcacgaccg ctttgagatc gctttcgatg
     7321 cggtacctga tcgagccgca aagacagctg ccttggtgta cctgaactgc ccgccaggaa
     7381 ggctcaagtg ccatcgtagt ccccaccgaa atggcgcaac ctactctagc accctgtggt
     7441 cgcgccgaat gcacagatag gctacggttt gctaaggtcg cttgcgtccc gccacacggt
     7501 cgcacgacac cgattgctgc agaggcaacc gcatgacaac ccatcgacag tccaatcccg
     7561 gcgaatcgac cgagcggcaa cgcctatcgt tgcgcaacct gtcacacaga gcgatgcgca
     7621 tctcctggtg ggacctgacg cagaccctcg gaccgatact gttactcggg gtgctcgggg
     7681 tactcctggc gttacatttc ggaggtcccg cgccaccaca ctcgatcagc attgcgagtg
     7741 gaccggaggg cagcaacttc gcgacgatgg cggcccgtta tcagaaagtg ctcaagcgca
     7801 acggcatcga actcaaggtc atcgataccg agggctcact cgataacctg aaacgcatgg
     7861 ccgatccgaa atcgcgcacc gacatcgcac tggtgcagtc tggccttgcc gaaggcggcg
     7921 acaacagcga cctggactcg ctgggaagcg tgttctatca agcgctcacg atcttctatc
     7981 gcagcccaaa gccgttgatg cggttgtcgg agctgcgcgg cggccgcatc gcgattggtc
     8041 cggaaggcag cggaacgcgt gcgctagccc tggcgttgct caaggcgaac gaaatagagc
     8101 cgggcgggtc cacgttgctg acgagtgacg agggcgaggg agcacgcgag gccctgctca
     8161 agcgagaggt ggatgcgatc tttctgaccg gagactccgc ctcgggtacg accattcgcg
     8221 aaatgctgca caccgaaggt gtgcggctgt tcgacttcga gcaagccgac gcctacaccc
     8281 gacgctttcc ctatctcaac aagctcgtag tgcccgccgg cgctttcgat ctgggtgagg
     8341 accttccggc aagcgacgtc aatctgctgg cgccaacggt cgaactcgtc gcgcacccga
     8401 atctgcaccc ggcactcgtt gatctgctga tcgaagcttc cgaagaggtg aacgggcgcg
     8461 cgtcgctgat gcagtcggcc ggacagttcc cgactcccgc gttgcacact tacccgatca
     8521 gcgatgaggc gatgcgctac tacaaatcgg gaaacaagag cttcgcctat cgctatctgc
     8581 ctttctggct cgccagcctc ttcaatcggc ttatcgtggt gctggtgccg ctattcgtgg
     8641 tggtggtacc cggcctgcga ttcttgcctg cgctctaccg atggcgcatc gcgacccgaa
     8701 tacatcgccg ctatggtgaa ctcatggcgg tcgagcgtga agccctaggt ccactcagcc
     8761 cagaacgacg gctaaccctg ctggagcggc tcgaggaaat cgagcgatcc gtcatttcgc
     8821 accggatgcc aggctcgcat gcggagcagg tctatgttct ccgggagcac atccgcttcg
     8881 taagagacaa cttggcgcgg ccgatcgtca caccactcga accagtcgct ggcggttgat
     8941 cagcgcagga acgaccttag aatacttgga tgtgcctcgc ccgcgccgaa aaacgaccgc
     9001 gctcaacgtt ctttgctgtg gccttcacag gttggctgac agcgctggcg tgcctgggcg
     9061 gctgctcgct gatcactatc aagagtccgg agaagccgct gtccacgcgt gacctgaacg
     9121 cgcggatcct cactcacgag tacgcagcgc acttcatcag cgcgatcgag cagaccgcgg
     9181 acgagatcgc ggacggcact caggataccc ggatacgact gaacaccctg cgatggaaga
     9241 tcgccgtggc ggcctccagt cagcgggctg ccagccagat cgtgccgatg atgagtctgc
     9301 tcgacacttg ggcgctgtcg gtacaaatgg cccagtatct ggacactggg aacggcgggg
     9361 ccctgttcgg cgcgcagcag tcccaggcgg tcacgctcgc cagggagctg gcgcatgacg
     9421 ccgagacctc cgcgcgaggg ctcctatcct cccaagagtt cacccgcgac cagcgcttcg
     9481 tcgaacacta tgcgcgagac tatccgcttc agagcctgaa ctttacgcga ccctcgattg
     9541 tggagcgatg ggtccgtgaa aacggctccg aatcgaaact catcgaatca ctgggtaccg
     9601 tgccggagtc gctggcggaa gcgcgcgacc tggtgcgcat gtacggtgat aacacgcccg
     9661 agcagatatt gtggaaggcg cagttgctcg cgcagcaatc aggcgtgaat ggtcgggatg
     9721 tgcagagcgc gctcaagcaa ttggacgagc gtatggcgcg gctgtcggct ctggcagatc
     9781 aaactccgga tttactcaat ggcatcgtgc gcgatgttca aagacgcgcc gatacttcct
     9841 ggtcgcagat gctgggcgag ttacgaactc agggtatgac gctgtcgggc acgctgagta
     9901 gcgaacgcga ggccgcgacg caatccatca acgaacagcg cgaagcgctg actgctgatg
     9961 ccgtgcggat atcgacccag ctggtccagc aggcaggcat cgaagcacga cggctggtgc
    10021 gagaagcgct gattctcatc attgtgctcg cgctcgttgt gctcggactg ccgttcactg
    10081 ccggctatct cgtcggacgc gcgcgccgct ctgcgtgaag aaatctcgac gcgcaatcac
    10141 ccggtcttcc ccgagagcgc gatcgatccg ggggttctcg tcagacattg aaacacccat
    10201 cgaaatggtg atacagacca gattgcttga caggagtgta ctcaaggctg gcgcatgaag
    10261 acgattggat tgatcggcgg gatgagctgg gaatccacca tcccctacta tcggatcatc
    10321 aatgagactg tcagggagcg tcttggtggg ctccactgtg ctcgagtgat cctgttcagc
    10381 gtcgatttcg ccgagattga gcgcttgcag caccagggtg actgggagca agccggtcga
    10441 tcgctggcgt tggccgctcg ctcacttgag cgtgcgggtg ccgacttcat ggtgttgtgc
    10501 acgaatacca tgcacaaggt cgcagccgcc atagagtcgg cggtctcgat tcctctactg
    10561 catatcgcag atgctgtcgc taccaccgtg actgcagcgg gccatcggtc cgtggggctg
    10621 ctcggcaccc gcttcacgat ggaacagccg ttttacaagg agcgcctgca ttcgcgcttc
    10681 ggactgtctg tactggtccc gcccgcgaac gatcagtcgc gcattcaccg catcatctac
    10741 gacgagctgt gcctcggcaa agtgctgcct cagtcccgcg ctgagtttca acgagtgatg
    10801 gacgatctgg tttcacgggg tgccgacgcc atgatcctcg gctgcacgga gatctcactg
    10861 ctcgtagggt ctgaggacgc gagtgttccg ctgttcgaca cgacccgcat acatgcgcag
    10921 atggcagcgg cgatggcgct tcagacgtca catcagacaa cctagggctt tatgggtgac
    10981 cggcgcgtca gctatcgcag gcggaagggc gtacaatgcg gtgatggcgt cttcgtccag
    11041 taggtttttc gtccgcctga agccagacct gtcatcacag gtggcgagcg agttccgtgc
    11101 gttcgcgaaa aagccgccgt ccggcaatcc gccaagtgac acccgactca ttctcgcgtg
    11161 cacccggatc gatttcagtc acagcgtctt cgccaaattg actatcaacc tcgacgacgc
    11221 agccgcaccg atcgatctct gggtacctca ttccctaatc ctaatgatcg gggatatcag
    11281 cgaaatggga cgcgctcccg ttgtatttac tccgacagag taaggcattg cgcgcgggcc
    11341 cagccgggcg cacttgtgcg cggtcctaat gacccctcag gttgcgcccc acttcttctt
    11401 ctgatcgcgc ttctcggcct tcaccgcccg cttctccatg agagtcttcg ccggcttctt
    11461 cctggtctcc ttcttgcgct cgttaccctt actcattgac tcctcctgct atgccggtga
    11521 cacgccttgc gaggcgcgca tagtctagaa ccaatctgtt cctgatgtct gggaatcgac
    11581 cgagctcaag actaccaaga aggtcttggc acttgtggat tcagtggctg acccagccga
    11641 tctcccgggc agtcataggc ccgacgcgct catcctcaag actgtcgggc aggcgtgctt
    11701 ccgatgcgct gagcggcatc cgtccagcca tgatctccgc gagctgctgc gggtagcgcg
    11761 gcgcaagctg cggctcggcg aaaccctctg ggtactgtgg caagcccgtg gcaagatcgt
    11821 atttatccgt ggctccgaag gtatgcagta gctcgtgggt gatcacgatg cggttctgcg
    11881 cctcctgcga gcggctggca aacacgtgca ccacggcggt cagcccgcgt tgcagcccgg
    11941 ccgagtgagg aaggctttgg gtcagctccg gatcatggta gagcagaaat agcgcaatct
    12001 tcgggcggga gcgggagacc gcggcgagcg cgcgggagcg gtaatagcgc agcttcaggc
    12061 tccagaggat gcgcgtgaac acgcccgatc ccgaatcgag ctccgggggc ggcactgtca
    12121 gagtcgggta gagcttcagc tcaatcggct catcggcact gactccataa cgccgggcct
    12181 cagccttcag gaagtcgctg acctcatcga ttttccgctg atcgagctgc gcgaggtagc
    12241 gcgcggtggc ggtgctcgca tcggcaccaa ccgggaacgc gccgacgtag accgtgcgct
    12301 gccagcgggt cgtgctaaac cggtcgagcc acgcctggcc ggccacgacc agcagcacca
    12361 gcagcaggac ggagatacgc aggcctcgcc acatggaaag ggttgtgtcc gcgccgtgag
    12421 gggttgtcga taatacggca agtgacggtc gcgtcgcgcg aaagatcctg caacgggacg
    12481 ggtcagcagt taccaagaaa cgcggcaccg acgcggcaaa tcaagggtat gttgtagctg
    12541 gactcactgg gcacgcgtat gtagaagcgc tcggcgctcc ggaaatgctc ccgtcgaagt
    12601 aagctggtga actccattat cgatgagcgg gctcggggtc ggcggatgca ccagaaggct
    12661 gagtggccgg caaggcggcg acgagcgagg ggctgatgac ttccaccgtg acgtgggaga
    12721 gagatggaaa actcttcagc agtgagtgat agaaggacgt atcgcgcgca cgcggcgtca
    12781 cgattgcaag gatggcgccc agatgtccgg gtcccaaccg ccacaggtgc aagtcggcga
    12841 tgcggtcacc gtcggcttcg aggcggcggc gaattcgacc cagcaacgcc gagtcaggcg
    12901 tcatgtcgag caggattcga cccgtgtcgc gcaccagagc gtaggcccag agcgagatga
    12961 ccagcgcgcc gacgatgccc atcatgggat ccatccagag ccaccc
//
LOCUS       DATMRM010000096         4258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_363854, whole genome shotgun sequence.
ACCESSION   DATMRM010000096 DATMRM010000000
VERSION     DATMRM010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363854"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VNZ06_02915"
     CDS             complement(<1..461)
                     /locus_tag="VNZ06_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19733.1"
                     /translation="MTTDLIAALNGASRNEMTRTNESKELLTTTQKARTINFDAPMYG
                     TFAEIGAGQEVAREFFHAGGASGTIAKSISAYDMVFSDAIYGKAPRYVSRERLNLMLD
                     HEYGLLVERLSAIRGERTNFFVFADTVATSNRPGANEAHGWLGIRFQSQPSG"
     gene            complement(458..2086)
                     /locus_tag="VNZ06_02920"
     CDS             complement(458..2086)
                     /locus_tag="VNZ06_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011788495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWW19734.1"
                     /translation="MSGAPSLLSRRRAPPAAAVGTGVSLLMGIAGCTLEPTYHRPEAR
                     VSQQWPGSPAVAVTPSAAAGKSTATPAATADIGWREFFVDARLQRLIELALQHNPDAQ
                     IAALNIAAARAQYQIQRADLFPSVAASATEQVDKYPPAVAGIAAAGGSSTGALRGSGS
                     GTFRYFEVGVGFTSYELDLFGRIRSLNHQRLQQYFGYVETRRSVRISLVAEVANAYLT
                     LLADQELLRITVDTLSSQEGSYKLTKMSYDGGIATALDLRQAETSVDTARANLAQYTR
                     QVAQDQNALVQLVGTALPADLPPGTGLDEQQLLADLPAGLPSDLLVRRPDILAAEHNL
                     IAANASIGAARAAFFPSLTLTGNYGTASTQLSGLFDHGSTAWTFSPQISLPIFAAGAN
                     VASLNLAKVEKDINIVQYEQAIQTAFREVADALAGRGTLDSQIAADQALVEATSDSFK
                     LSDKRFRDGVDDYLSVLDSQRSLYTAEQTLIGVKLARLQNLVTLYKALGGGWSERGAQ
                     NDKVTDSTHAVSGNNTTNVTPVISGLPTNPEIFQ"
     gene            complement(2083..>4258)
                     /locus_tag="VNZ06_02925"
     CDS             complement(2083..>4258)
                     /locus_tag="VNZ06_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW19735.1"
                     /translation="TTPFVRISIKEVVKTLFEAIGLVFLVMLLFLQNIRATFIPTIAV
                     PVVLLGTFGVLQAFGYSINVLTMFAMVLAIGLLVDDAIVVVENVERVMHEDGLSARDA
                     TRKSMDQITGALVGIALVLSAVFLPMAFFSGSTGVIYRQFSITMVSAMTLSVLVALIL
                     SPALCATLLKPPEKGLRKQRGLFGWFNRNFERGSQKYERAVVGVVGRTGRYLIVFVVI
                     VGLMALLFTRIPRSFLPDEDQGVLFAQVTTPPGATSERTGLALAEVRDYFLNDEKQAV
                     EGVFTVSGFNFAGHGQTSGLVFVRMKPWDDRPGAHNRVQAVAARANQRLQKIADAQAF
                     AFAPPAALELGNATGFDLELLDRANQGHDKLMQVRNDLLGVARKDPQLQQVRANGLDD
                     EPQYKFDVDWEKASALGLTISDINNTLSAAWGSQYVDDFIDRGRVKRVFIQGDVPSRM
                     LPQDLTDWYVRNSASQMVPFSAFGAAAWAYGSPKLERYNGVPAVEILGSPIAHVSTGT
                     ALDAMATYAKKLPPGFDLEWTGLSYEERLSGSQAPALYAISLIVVFLCLAALYESWSI
                     PVAVMLVVPLGVIGAILATLLRGLANDVFFQVGLLTTVGLSAKNAILIVEFAKENHDH
                     GMELIEATVQAARQRLRPILMTSLAFILGVLPLAISRGAGSGGQNAIGTGVIGGMLSA
                     TVLAIFLVPVFFVATRKLFVGNPTQHDSPVPLHPGEEAEQPT"
BASE COUNT          842 a         1320 c         1293 g          803 t
ORIGIN      
        1 ccgctgggct gagattggaa ccgtataccc agccagccat gtgcttcgtt tgcacccggg
       61 cggttactgg tcgcaaccgt gtccgcgaag acgaagaaat tcgtgcgctc gccgcggatt
      121 gccgacaggc gctcgacgag aagcccgtat tcgtgatcca gcatcaggtt caagcgctcg
      181 cgcgacacat accgcggcgc tttcccatag atggcatcgc tgaacaccat gtcgtaggct
      241 gagatggact ttgcaatcgt ccccgacgcc ccgccggcgt gaaaaaactc acgcgcgacc
      301 tcctggcccg ccccgatctc cgcaaacgta ccgtacatcg gtgcatcgaa gtttatcgtg
      361 cgtgctttct gtgtcgtagt gagcagctcc ttactctcgt tcgtgcgcgt catctcattc
      421 cggcttgcac cgttcagggc agcgatcaaa tcagtagtca ttggaatatc tcagggttag
      481 ttggaagtcc gctaatcacg ggggtgacat tggttgtgtt gttgccggag acagcgtgag
      541 tactgtccgt cactttgtca ttctgcgcgc cgcgctcact ccagcccccg cccagcgcct
      601 tatacagagt cacgagattc tgcagtcgtg ccaacttcac tccaatcagg gtctgctcgg
      661 cggtgtacag cgaccgctgt gagtcaagca cgctcagata gtcgtcaacg ccgtcgcgga
      721 atcgcttgtc tgacagcttg aagctgtcac tggttgcctc gaccagcgcc tggtccgccg
      781 cgatctgact atcgagcgta cctcgaccgg caagggcatc ggcgacttcg cgaaatgccg
      841 tttgaatcgc ctgctcatac tgcacaatgt tgatgtcttt ttcgactttc gccagattga
      901 gtgaagccac gttggcgccg gcggcgaaga tcggcaggct gatctgcggt gaaaacgtcc
      961 aggcagtgga gccatgatca aagagccccg acagctgggt gctggcggtg ccgtaattgc
     1021 cggtgagcgt gaggctgggg aagaatgccg cacgtgccgc accgatgctg gcattcgccg
     1081 cgatcagatt gtgctcggcg gcgagtatgt cgggccggcg caccagcaga tcggagggta
     1141 ggcccgccgg cagatccgcc agcagctgct gttcgtcgag tccggtgccc ggcggcaggt
     1201 ccgccggcag cgccgtacca accagctgca ctagcgcgtt ttgatcctgc gcgacttggc
     1261 gcgtgtattg cgcgagattg gctcgcgcgg tgtcgacgga cgtctccgcc tgacgcagat
     1321 caagcgcagt tgcgataccg ccgtcgtaac tcatcttggt gagtttgtag gatccttcct
     1381 ggctgctcag cgtgtccacc gtgatgcgca gcagctcctg atccgcgagc agcgtcagat
     1441 aggcgtttgc cacctccgcc accaggctga tccgcacact gcggcgggtt tcaacatagc
     1501 cgaaatactg ctgcagtcgc tgatgattga ggctgcggat tcgcccaaat agatcgagct
     1561 cgtaggaggt gaagccgacg ccgacctcga agtagcgaaa agtgccggag ccgctgcctc
     1621 tgagcgcacc agtggagctg ccgccggcgg cagcgatacc ggccacagcc ggcggatatt
     1681 tatcgacttg ctctgtagcg cttgccgcta ctgatggaaa caaatcagcg cgctgaattt
     1741 gatattgagc gcgcgccgcc gcgatattga gcgcagcgat ctgcgcatcc gggttgtgct
     1801 gcaatgccag ttcaatcagc ctctggagcc gcgcgtcgac gaagaactca cgccagccga
     1861 tgtcggccgt cgctgccggc gtcgcggtac tctttccggc cgccgcgctc ggtgtcacgg
     1921 cgactgccgg gctgccaggc cattgctggc tcacccgcgc ctcaggacga tgatatgtcg
     1981 gctcgagcgt gcaaccggcg attcccatca gcaggctgac gccggtgccg acggcggccg
     2041 ccggcggtgc gcgccgcctg cttaacaggg acggagcgcc gctcacgtcg gctgctccgc
     2101 ttcctcaccg gggtgcaacg gcaccggcga atcgtgctgc gtggggttgc cgacgaacag
     2161 cttgcgcgta gcgacaaaaa ataccggcac aaggaaaatg gccaaaaccg tcgctgacag
     2221 catgccgcca atgacgccgg tgccgatcgc gttctggcct ccggatccgg cacctctgga
     2281 aatcgcgagc ggcagcacac cgagtatgaa ggcgagtgag gtcatcagaa tcgggcgcag
     2341 acgctgccgc gcggcttgca cggtcgcctc gatcagctcc atgccgtgat cgtgattttc
     2401 tttggcgaac tcgacgatta gaatcgcatt cttcgccgac agacccacag tcgtcagcag
     2461 accgacttgg aagaacacgt cgttcgccag tccgcgcagc agcgttgcaa ggatggcgcc
     2521 gatcacgcca agcggcacaa cgagcatcac cgccacaggg atcgaccagc tctcgtagag
     2581 cgcggctaga cacaggaaca ccacgatcag cgaaatggca tacaacgcag gggcttgcga
     2641 ccccgacagg cgttcttcat acgacagccc ggtccactcc aaatcgaagc ccggcggcag
     2701 cttctttgcg tacgtcgcca tggcatcgag ggcggtgccg gtactgacgt gggcgatcgg
     2761 cgagccgaga atttccaccg ccggcacgcc gttgtagcgt tccaacttgg gagatccata
     2821 agcccatgca gcagcgccaa aagccgagaa cggcaccatc tgcgaggccg agttacgcac
     2881 gtaccaatcg gtcagatcct gcggcagcat gcgcgagggc acgtcgccct ggatgaacac
     2941 gcgtttgacg cggccgcggt cgatgaagtc gtcgacatat tgcgaacccc aagcggccga
     3001 cagcgtgtta ttgatatcgc tgattgtgag ccccagcgcg ctggcttttt cccagtcgac
     3061 gtcgaatttg tactgaggct cgtcatcgag cccgttggcg cgtacctgct ggagctgcgg
     3121 atccttgcgc gccacgccga gcaggtcgtt acgcacttgc atcagcttgt cgtgcccttg
     3181 attggcgcga tcgagcagtt caaggtcgaa gccggtggcg ttcccaagtt ccagcgcggc
     3241 agggggtgcg aacgcaaacg cctgagcatc ggcaattttc tgcagccgtt ggtttgcacg
     3301 agccgcgacc gcttgcacgc gattgtgcgc gccgggccga tcgtcccagg gtttcatgcg
     3361 tacgaacaca aggcctgagg tttggccgtg accggcgaag ttgaagccgg agacggtgaa
     3421 tacaccctcg actgcctgct tctcgtcatt gaggaagtag tcgcggactt cggccagcgc
     3481 taacccggtg cgctcgctgg tcgctcccgg cggcgtagtg acctgcgcaa atagaacgcc
     3541 ctggtcctcg tcgggcagaa acgacctcgg gatgcgagtg aatagcaggg ccattaatcc
     3601 gacgatgacg acaaacacaa tcaggtagcg gccggtgcgg ccgaccacgc ctaccaccgc
     3661 gcgttcgtac ttctggctgc cgcgctcgaa gttgcgattg aaccagccga ataaacctcg
     3721 ctgcttgcga agtcccttct caggcggctt gagcagagtg gcgcacaggg ccgggctcaa
     3781 gatcaatgcc accagcaccg acagcgtcat agccgacacc atggtgatgg agaactgtcg
     3841 atagatcacg ccggtggacc cgctgaagaa cgccatcggc aagaacacgg ccgatagtac
     3901 gagcgcgatt ccgaccagcg cgccggtgat ttggtccatc gacttgcgcg tcgcgtcgcg
     3961 cgccgacaga ccatcctcgt gcatcactcg ctcgacattc tcgaccacga caatggcatc
     4021 gtcgaccagc aggccaatcg cgagcaccat ggcgaacatg gtgagcacat tgatcgaata
     4081 cccaaaggcc tgcagcaccc caaatgttcc gagcagcacc accggtaccg cgatggtcgg
     4141 gatgaaggtt gcgcggatgt tctgcaggaa cagcagcatc acgaggaaca ccaaaccgat
     4201 cgcttcgaac aatgtcttga ccacctcttt gatcgagatg cgcacgaatg gcgtagtg
//
LOCUS       DATMRM010000097        12406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1289211, whole genome shotgun sequence.
ACCESSION   DATMRM010000097 DATMRM010000000
VERSION     DATMRM010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12406
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289211"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VNZ06_02930"
     CDS             <1..295
                     /locus_tag="VNZ06_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19736.1"
                     /translation="QWPKFTAYIPDLISAVQARIDVLAKNKKEAAKVDLPTAQADMAD
                     VNTLWARAQQAFTGGDPETAGETTREVEKKINAAAAAIKFTLPRPGAATAPAH"
     gene            306..992
                     /locus_tag="VNZ06_02935"
     CDS             306..992
                     /locus_tag="VNZ06_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWW19737.1"
                     /translation="MAQALLETVEVETGRNPTASVIWMHGLGADGHDFEPAVPHLADS
                     IGQPVRFVLPHAPMRPVTINAGMRMRAWYDVLGFDRQSGQDETGIRASAAAVGALIQR
                     ENERGIVNGRIVLAGFSQGGAMSLYIGARYPAKIAGIVALSCYLPLASNFAAERLAAN
                     QSIPIFMAHGSLDAVIEEQRGLEARAGLETAGFEVEWHSYRMAHAVCVEELADISAFL
                     ERVLSVGLNH"
     gene            1112..3937
                     /locus_tag="VNZ06_02940"
                     /pseudo
     CDS             1112..3937
                     /locus_tag="VNZ06_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262356.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
     gene            4009..5223
                     /gene="odhB"
                     /locus_tag="VNZ06_02945"
     CDS             4009..5223
                     /gene="odhB"
                     /locus_tag="VNZ06_02945"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020299225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HWW19738.1"
                     /translation="MSVQIRVPQLPESVADATLVAWRKQPGQAVTRDENLADLETDKV
                     VLEVPAPADGVLKEIKVASGTTVKSGDLLAVIEEGAAASAPAASAPAAAGNGKAPATA
                     VASPAVATSRPAADSSKAGPAARRVMEQNQLSAGAIVGSAPGGRITKTDAVAAAAAAS
                     TKPAVPAAAGTGRTDQRVAMTRLRARIAERMVQAQATQALLTTFNEVDLTAVQELRAR
                     HKDSFEKKHGVKLGFMSFFVRASVDALKRFPVVNASVENADIVYHEYFDIGVAVSTDR
                     GLVVPILRDADAMSFADIEKGIADYAARARKGSLGLEDLTGGTFSITNGGVFGSLMST
                     PIVNAPQSAILGMHKIQDRPMVVDGQVKVRPMMYLAVTYDHRIIDGRDAVQFLVAIKD
                     CLEDPGRLLLGV"
     gene            5229..6653
                     /gene="lpdA"
                     /locus_tag="VNZ06_02950"
     CDS             5229..6653
                     /gene="lpdA"
                     /locus_tag="VNZ06_02950"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWW19739.1"
                     /translation="MADAYDVVVIGGGPAGYPCALRAAQNGLKVACVDEWKNRDDKPA
                     FGGTCLNAGCIPSKALLESSELVYRAQHEFAAHGIKTDVSIDLATMQKRRATIVRNMT
                     GGIASLFKSGNVTGIFGHGRLLSGRRIEVTAHDGSKSEVTARHVVLASGSIPTELKSL
                     TFDHQSILDSWDGLELTAVPKTLCVIGAGVIGLELGSVWRRLGAQVVVLEALENFLAI
                     ADQQLANEAARHFKKQGLDIRLGARVSGASVASNGVTVSYSDKTGAQSVVAEKVIVAV
                     GRRPFTANLFAADTGVALDERGFIKVDDHCHTDAEDIWAIGDCVRGPMLAHKGKEEGI
                     AVADAIAGNYAHVNYDVIPSVIYTAPEIAWVGQTEEQLKAASIPYKSGVFPFAASGRA
                     RAMEAAVGFAKILAARDTDTILGVHIVGPMAGELIAEATLAMEFQASAEDLQRTIHAH
                     PTLAEALHEAALAADKRAIDIMNR"
     gene            6653..7372
                     /locus_tag="VNZ06_02955"
     CDS             6653..7372
                     /locus_tag="VNZ06_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HWW19740.1"
                     /translation="MSPLLFREDAYLTTATARVVAVHERGIELDRTIFYPMGGGQAGD
                     SGTLLRSNGERIRIADSRKGESVDSVIHIPAVGAPVPQSGEELQLEIDWQRRYALMRL
                     HTALHVMSCVVVAPVTGGNISPDKGRLDFDIDMSLLDAKAIEQGTNALIARAVPTETV
                     WITDAELDARPELVKTMSVQPPRGSGRVRLLRIPDVDLQPCGGTHVRNIGEIGAIRVL
                     RIRSEGKHNKRVEIALASSSP"
     gene            complement(7605..9725)
                     /locus_tag="VNZ06_02960"
     CDS             complement(7605..9725)
                     /locus_tag="VNZ06_02960"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HWW19741.1"
                     /translation="METATQEAPEIAAHLDAARAVLREYGADPAALDLGAEAAHTVAA
                     LTRDTDLAIGTLLHGARTAGLPRDIRQLETRLGANPSRLAAELERLGELHLPTGWSAS
                     QGLTAQQAEILRKMLLAVAADPRLVVARLAEQLVQLRHARGRPPQEQRRLASETREVL
                     APLANRLGVWNLKWELEDLAFRYLEPEDYHRIARALAERRVDRERYIVEVCALLRREL
                     EQAGVTALAVYGRPKHIYSIARKMRRKHIAFEQLYDVRAVRIVVESVSDCYAALGVVH
                     GLWHYIPGEFDDYIATPKDNEYRSIHTAVTGPEGRSLEVQIRSQDMHEYAELGVAAHW
                     RYKEGGARDLDYERKIEWVRRVLDPTQQTHSEGDLIERMKGELFADRIYAMTPRGEVV
                     DLPRGATALDFAYHVHTDLGHRCRGAKVDGRIVPLNQPLTNGGVVEIITGKQPAPSRD
                     WLSPEQGFLASSRSRAKVRAWFRRIDQSEHLTSGRTILERELTRAGTGQEVIAAVVRE
                     LHAGSTEDLQRMLGAGEITPATLSATLSRLRAPETPPPAPRKRPKRTSARSPIEIEGV
                     GDLPTTLARCCSPVPPQPIAGYVTLGRGVTIHRADCPNLLRMRAHNTTRMLNVEWSLD
                     SDSLLPVQIRVEALDRRGLLRDVTDVMALERLSIDGVNSDTDPDDRVATIVMRTAVRD
                     VDQLARVLQRLTGVPNVLRAQRLT"
     gene            9802..10392
                     /gene="ruvC"
                     /locus_tag="VNZ06_02965"
     CDS             9802..10392
                     /gene="ruvC"
                     /locus_tag="VNZ06_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HWW19742.1"
                     /translation="MARYAGKTWRALRPTDIPTRASCLPVRLHSPDCRVLGLDPGSQR
                     TGYGVIDCQAGAERHLTSGCIDVTGQDMVVRLQCIYRSVVALVHEHRPDMIAIERVFV
                     HRNPDSALKLGQARGVALCAAASLGASVHEYAPRAIKMAITGYGAAAKPQVAQMVRNL
                     LMLEIKLTPDAADALAVALCHSQSRRMGALAAADVA"
     gene            10408..11034
                     /gene="ruvA"
                     /locus_tag="VNZ06_02970"
     CDS             10408..11034
                     /gene="ruvA"
                     /locus_tag="VNZ06_02970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWW19743.1"
                     /translation="MIGSLSGVLVVKSPPHLLLEVGGVGYELEAPMSTFFVLPEVGER
                     ARLLTHLQVREDAHVLYGFSTAAERTLFRNLLKVSGVGPRIALAILSGVSAASFAEAV
                     RNEDSAMLTRIPGVGRKIAERLIVEMRDRIESESAFSVAGMATPSTGIPLPIMGGGAQ
                     REAFSALVAFFGYKPAEASRLLKAVEPDSGSLSTEEWIRRALQSAVRA"
     gene            11031..12080
                     /gene="ruvB"
                     /locus_tag="VNZ06_02975"
     CDS             11031..12080
                     /gene="ruvB"
                     /locus_tag="VNZ06_02975"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HWW19744.1"
                     /translation="MNAHEDDRLVSTAAGGGEELIDRAIRPRLLNDYIGQAAVKEQLS
                     IFIDAARMRSEALDHVLIFGPPGLGKTTLAHIIAAELKVNLRQTSGPVLEKPGDLAAM
                     LTNLQPRDLLFVDEIHRLSPVVEEILYPAMEDYQLDIMIGEGPAARSIKLSLPPFTLV
                     GATTRAGLLTSPLRDRFGIVQRLEFYSVSDIERIVHRSARILEVAIEPEGAKRIAQRS
                     RGTPRIANRLLRRVRDYAQVKGSGHIDEACAHAALDLLQIDRQGLDALDHKLLRTIME
                     KFDGGPVGIDSLSAAIGEERGTLEDVIEPFLIQQGFIMRTARGRQATRLAWQYLGLTV
                     PERAVANLSLFGEES"
     gene            12083..>12406
                     /locus_tag="VNZ06_02980"
     CDS             12083..>12406
                     /locus_tag="VNZ06_02980"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HWW19745.1"
                     /translation="MFAWQARVYWEDTDAGGIVYYGNYLRFLERARTEWLRAKGVWQR
                     KLEQEAGVIFTVIDLQVKYRLPARLDDLLWISCEPAREGGATIAFAQRVWREHVGGEL
                     LLEGVA"
BASE COUNT         2215 a         3779 c         4123 g         2289 t
ORIGIN      
        1 gcaatggccg aagttcaccg cctacatccc ggatctgatt tctgccgtgc aggctcggat
       61 cgatgtgctg gccaagaaca agaaggaggc cgccaaagtg gatctgccga cggcgcaggc
      121 ggacatggcc gacgtcaaca ccctgtgggc cagggcgcag caggccttta ctggcgggga
      181 tccggagacg gccggcgaga ccacgagaga agtcgagaaa aaaatcaacg ccgcggccgc
      241 tgcgatcaaa ttcaccctgc cacgcccggg ggcggcaacg gcaccggcac actaagacag
      301 aacgcatggc gcaggcactg ctcgagacgg ttgaagtcga aacgggtcgc aatcccaccg
      361 cgtcggtcat ctggatgcac ggcctggggg ccgatggtca cgatttcgag ccggcggtgc
      421 cacatctggc cgattcgatc gggcagccgg tgcgatttgt gttgccgcac gcgccgatgc
      481 gcccggtcac catcaacgcc ggcatgcgca tgcgcgcctg gtacgacgtc ctcggattcg
      541 atcgccagag cggccaggat gaaaccggca tacgggcctc agccgctgcg gtcggtgcac
      601 tgattcagcg cgagaacgaa cgcggcattg ttaacggccg gatcgtcttg gcaggttttt
      661 cacaaggcgg agcgatgtcg ctttacatcg gtgcgcgcta cccggcgaag atcgccggta
      721 tcgttgccct gtcatgctac ctgccattgg cctcgaactt cgctgccgaa cgccttgcgg
      781 cgaatcaatc catccccatc ttcatggctc acgggtcgct cgatgcggtc atcgaggaac
      841 agcgtgggct tgaagctcga gccgggctcg agacggcggg ctttgaggtg gagtggcaca
      901 gctatcggat ggcgcacgcg gtctgcgtgg aagaattggc cgacatttcg gcgttcctgg
      961 agcgggtgct gtctgtcggt ctgaaccact gagcgtgaac gttgtccgtt tctttagtca
     1021 acccgttcct atagaatacg aggtccacta ttagcatgta gctcattgaa ctaacaggta
     1081 tttttcaagt tttcctgtca cggtaccccc catgccgaaa actcttcccg agctgctcgc
     1141 gagcacaccg ctatcgggcg gcaacgcttc ctatgtggaa gggttgtatg agcagtatct
     1201 aagtcagccc gctgacgtca gcgacgaatg gcgcgactac ttcgatgcgc tgggggcctc
     1261 cgcacgaccc gagcgtgcgc atagcccgct cattgcggcg atcgccgagc gaacccggca
     1321 gcctgccatc cctcgtaccg cgccggctgc ggccagcggt gcggccagcc agaagcaggc
     1381 cgcggtctcc cgcctggtcc agctctacat caatcgcggc cacctggtgg cgcggatcga
     1441 tccgctcgat ctgctgcagc gtccgcggcc gcgcgtgctg gatctggagt atgcgggact
     1501 gagcgaggct gatctcgata cggagttcta caccgccgcg cgcaatgaat ggataccgaa
     1561 gcaggccacg ctgcgcgaga tcctctcgcg actgcagcag gtgtactgcg gacacatcgg
     1621 cgccgaattc gcccacattt cggacagcga cgagcgactg tggctgcagg acgaatttca
     1681 gctcggtcgc atgcaacatc gcttcagcac cgacgagcgc cgcaatctgc tgtcgcaact
     1741 gaccgcggcg gagggtctgg agcgttatct gcacaccaag tacgtcggcc agaagcgctt
     1801 ttcactcgaa ggtggcgatg cgctcatcgc ctgcctcaat gacctgattc agcagggcgg
     1861 ccgggtgggc atcgaggaat tcgtcctggg gatggcgcat cgcggtcgtc tcaacgtgct
     1921 ggtgaatgta ctgggcaaat ccccggcgga gctgttttcc gaattcgagg gcaagtacga
     1981 cgtcaatcac atgcagggct cgggcgacgt taagtaccac aagggcttct cggccgacgt
     2041 acgcactgcg gcaggcaacg tgcacgtcgc gctggcgttc aatccctcgc atctcgagat
     2101 cgtcaatccg gtggtcgaag gctctgtgcg cgcccgccag gaacggcgca ctgacgccca
     2161 cggcgacaat gtcgtgccgg tgctgatgca cggcgatgca tcgttcgccg gacagggcgt
     2221 ggtgatggag acgctgcagc tttcgcaagc gcgcggctac cgcaccggcg gcaccattca
     2281 catcatcgtc aacaatcaga tcggctttac gacctccgat ccacgcgacg cgcgctcaac
     2341 cacctatgcg agcgacgtcg ccaagatcat tgaagccccg gtgctgcacg tcaacgccga
     2401 cgatccggaa gccgtggtgt tcgtcacccg actggcgctg cactatcggc agaaattcca
     2461 caaggatgtc gtgatcgatc tggtgtgcta ccgaagactg gggcacaacg aagcggacga
     2521 gccggcggcg acccaaccgg tgatgtatcg ggtgattcgt tcgcatccga ccgtgcgcag
     2581 actgtacgcc gatcaactgg tggccgatgc ggtgatctcg caggcagagg ccgattctat
     2641 ggccgcgcag tatcgccagg cgctcgattc cgggcgtctg gaactgcatc cgacgctggg
     2701 catggtgggc aacaagtaca cgatcgactg gtcgcgctac tcacacttcg atctgtcgga
     2761 gcgcatgcgc acgggcgtag accctgcgcg acttgcggca ctgggcaagc gcatcacgca
     2821 gcatccggcc gacctgtcgc tgcaaccgcg cgtgactcag atcatggcca atcgagccaa
     2881 aatggttgct ggcgaaatgc cgctggactg gggagcagcc gaaaccctgg cctatgcctc
     2941 gctgctggat cagggcacgt ccgtgcgtat ctccggccag gatagtggtc gcggcacatt
     3001 tttccaccgg cacgcggtgt tgcacgatca gaacaccggc cgacgcttca ttccgctgca
     3061 gcatgtgaaa gatgatcagc ctcgtttcac cgtgatcgac tcggtgctgt cggaagaggc
     3121 ggtgctcgga ttcgaatacg ggtacgccac caccagtccg gattgcctcg tgatctggga
     3181 ggcgcaattc ggcgacttcg tgaatggtgc gcaggtcgtg atcgatcagt tcatttcctc
     3241 gggtgaggcg aaatggggtc gtctgtgcgg actggcgatg ttcctgccgc atggctttga
     3301 gggtcagggc ccggagcatt cctccgcgcg gctggaacgc ttcctgcagc tgtgcgccga
     3361 gaacaacatg caggtcgtgg tcccgtccac gccggcacag atatttcacc tgatccgccg
     3421 ccagatcatg cagccgttcc ggcggcccct gatcgtgttc atgcccaaga gtctgctgcg
     3481 tcacgagttg tcggtctcct cgctcggcga tctgagtaac aatagtttcg cgcacatcat
     3541 cgatgaggtg gacgatgtgc cagcggccgc ggtgcaacgt gtggtgttct gcagtggcaa
     3601 ggtgtatttc gatctgctga agcagcgccg tgcggaagct ctgagtcaca ttgcgctggt
     3661 gcgtatcgag cagctgtatc cgtttccggt agaagaatat caggcggtgc tgacgcgcta
     3721 cgccaacgcg accgacatcg tctggtgtca ggaggagccg cagaatcagg gtgcctggta
     3781 tcagatccgg caccgcctgc aggatctcgc gggcgctcgc tcggtctact acgcgggcag
     3841 ggccgcatcg gcggcaccgg ccacaggttt gagcaagatt cacgaggcgg agcagaagcg
     3901 cctagttgac acggcgctca atgcgatctc gatcgagcat acgatacgca acactccgcg
     3961 gctcaccatg acgccgagcg gtgcgattgc gcccataagg aagagttcat gagtgttcag
     4021 atccgtgtgc cgcagttgcc tgagtcggtc gccgacgcca cgctggtagc gtggcgcaag
     4081 cagcccggcc aggcagtcac ccgcgatgaa aacctggctg atctggagac cgataaggtc
     4141 gtgctggaag taccggcccc ggcggacggc gtcctgaagg agatcaaggt cgcgtccggt
     4201 accaccgtca agagtggtga cttgctggcg gtgatcgaag agggagcggc tgcctcggcg
     4261 cccgcggcat cagcgccggc cgctgcgggt aatggcaaag cccccgccac agcggtggct
     4321 tcgccagcgg tggccacatc acggcctgcc gccgattcct ccaaggccgg tcccgcagcg
     4381 cggcgggtga tggaacagaa ccagctttcg gcgggcgcga tcgtcggatc ggcaccgggt
     4441 ggccgtatca ccaagaccga tgcggtcgcc gcggcggcag cggcctcgac caagcctgcg
     4501 gttcccgctg cagccggtac cgggcgcacc gatcagcgcg tggcgatgac gcgactgcgg
     4561 gcgcgcattg ccgagcgcat ggtacaggcg caggccacgc aggcgctgct gacgaccttc
     4621 aacgaagtgg acttgaccgc ggtgcaggag ctgcgtgcgc ggcacaagga ctcctttgaa
     4681 aagaagcacg gcgtgaagct cggtttcatg tcgttcttcg tcagggcgtc ggtcgacgcg
     4741 ctcaagcgct tcccggtggt caatgcatcg gtggagaacg ctgacattgt ctatcacgag
     4801 tactttgata tcggagtcgc ggtctcgacc gatcgcggcc tggtcgtgcc gatcctgcgc
     4861 gatgccgacg cgatgagttt tgccgacatc gaaaagggga ttgccgacta cgcggctcga
     4921 gctcgcaagg gctcgctggg gctcgaagac ctcaccggcg gcacattcag catcaccaac
     4981 ggcggcgtgt tcggttcgct gatgtccact ccaatcgtga atgctccgca aagcgcgatc
     5041 ctgggcatgc ataagatcca ggatcgcccc atggtcgtgg atggccaggt gaaagtgcgc
     5101 ccgatgatgt atctggcggt gacctacgat catcgcatca tcgacggccg cgacgcggtg
     5161 caattcctgg tcgctatcaa ggactgccta gaagatccag gccgtctgtt gctcggagtc
     5221 tgatcctcat ggctgatgca tacgatgtcg tcgtgatcgg cggcggcccg gcgggttacc
     5281 cgtgcgcgct gcgtgccgca cagaatggtc tcaaggttgc ctgcgtggat gagtggaaga
     5341 accgcgatga caagccggcc tttggaggca cctgtctgaa tgcggggtgc attccttcca
     5401 aggcgttgct ggagtcgtcg gaactggttt accgcgcgca gcatgagttc gccgcgcacg
     5461 gcatcaagac cgacgtcagc attgatctgg cgacgatgca gaagcgccgc gctacgatcg
     5521 tgcgcaacat gaccggcgga attgcctcat tgttcaaatc gggcaacgtg accggcatct
     5581 tcgggcatgg cagactgctg tccgggcgcc gaattgaagt caccgcacac gatggcagca
     5641 agagtgaagt cacggccagg catgtggtgc tggccagtgg ctcgatcccg accgagctga
     5701 agtcgctgac tttcgaccac cagtcgatcc tcgattcgtg ggacgggctg gagctgacag
     5761 cggtaccgaa gactctgtgc gtcatcggcg cgggcgtgat cggcctggaa ctgggcagcg
     5821 tctggcggcg gctcggagca caggtcgtgg tgctcgaagc gttggagaat ttcctggcga
     5881 ttgccgatca gcagctggcc aatgaggccg ctcggcactt caagaaacag ggtctggata
     5941 tacgtctggg tgccagagtc agtggtgcca gcgtcgctag caacggcgtc acggtcagct
     6001 acagtgacaa gacgggcgcg cagagcgtgg tggcggaaaa agtgatcgtc gctgtgggcc
     6061 gccggccgtt taccgcgaac ctgtttgcgg ccgataccgg cgttgcgctg gatgagcgcg
     6121 gattcatcaa ggtggacgat cattgtcaca ccgatgccga agacatctgg gcgatcggcg
     6181 attgtgtgcg cggcccgatg ctggcacaca agggcaagga agaaggcatc gccgtggctg
     6241 atgccattgc tggcaactac gcgcacgtca actacgatgt gattccgtcg gtgatctata
     6301 ccgcaccgga aattgcctgg gtcgggcaga ccgaagagca gctcaaagcg gccagcatcc
     6361 cctacaagag cggcgtgttt ccgttcgctg ccagcggtcg cgcgcgtgcc atggaggcgg
     6421 ccgtcgggtt cgccaaaatc ctcgcggcgc gcgacaccga tacgattctg ggcgtgcaca
     6481 tcgtgggacc gatggcgggc gagctgatcg cggaggccac actggcgatg gagtttcagg
     6541 ccagcgccga ggatctgcag cgcacgatcc acgcgcatcc gacactggct gaagcactgc
     6601 atgaggcggc actcgcggcc gacaagcgtg ccatcgacat catgaaccgc tgatgtcgcc
     6661 actgctgttc cgcgaggatg cgtatctgac gaccgccacg gcgcgggtgg ttgcggtcca
     6721 cgagcgtggc attgaactgg atcgcacgat cttctatccg atgggcggcg ggcaggccgg
     6781 tgacagcggc acgctgctgc gatccaacgg cgagcgcatc cggatcgctg acagccgcaa
     6841 gggtgagagc gtggacagcg tgattcacat tcccgcggtg ggtgcccccg tcccacagtc
     6901 aggcgaagag ctacagctcg agattgattg gcagcgtcgt tatgcgttga tgcgattgca
     6961 taccgcgctg cacgtcatgt cctgcgtggt ggtggcaccg gtgaccggcg ggaacatctc
     7021 gcccgacaag ggacgcctgg acttcgatat cgacatgagc ctgctcgatg cgaaggcgat
     7081 cgaacaggga accaatgcgc tgattgcccg ggcggtgccg accgaaacgg tctggatcac
     7141 ggacgcggag cttgacgccc ggcccgaact ggtgaaaacc atgagcgtgc agccgccgcg
     7201 tggcagcggt cgggtgcgtc tgctgcgcat accggacgtc gatctgcagc cctgtggcgg
     7261 cacgcatgtg cgcaacattg gcgagatcgg cgcgatccgc gtgctgcgca ttcgcagcga
     7321 aggcaaacac aacaaacgcg tcgagatcgc gcttgccagc tcaagtccct agcggttcac
     7381 gctggtcatc atcgccgggt tgtagcgcgc tccggcggcc gcgccatgcg ggatcacatc
     7441 actgagctgt tcgacttgct tcgccgtgag cgtcagggcc gcggcctttg cgttttcatc
     7501 cagtcgtgtc gtgctgctgg taccgggaat gggaaccaga gcggatggcc gaggactcta
     7561 cacgtccccg caaggccggt cacgtcggac gatgtcggcg aatctcaggt caggcgttgc
     7621 gcccgcagta cgttcggcac gccggtcaga cgctgcagca cgcgcgccag ttgatcgacg
     7681 tcccgcaccg cggtgcgcat gacgatggtc gcaacgcgat catccgggtc ggtgtcggag
     7741 ttgacgccgt cgatgctcag gcgctcgagt gccatcacat ccgtgacatc gcgcaacaaa
     7801 ccacggcggt cgagtgcttc gacacgaatc tgcaccggca acaggctatc ggagtccaga
     7861 ctccactcga cgttcagcat ccgcgtcgtg ttgtgcgccc gcatgcgcag cagattcggg
     7921 caatccgcgc gatggatcgt cacgccacgc cccagagtca catagccggc gatcggctgc
     7981 gggggtaccg gcgagcagca gcgtgcgagg gtggtgggca gatcgccgac accttcgatc
     8041 tcaatcggag agcgcgccga agtgcgcttg ggccgtttgc gcggcgcggg aggaggagtc
     8101 tcgggtgcgc gcagccgcga cagagtcgcc gacagcgtgg ctggggtgat ctcaccggct
     8161 ccgagcatgc gctgcagatc ctcggtgctg ccggcatgca gctcacgcac cacggcggcg
     8221 atgacttcct gtccggtgcc ggcgcgggtc agctcacgtt cgaggatcgt acggccgctc
     8281 gtgaggtgct cggattgatc gatgcgccga aaccaggccc gtactttggc gcgactgcgc
     8341 gatgaggcca gaaacccctg ctcgggtgag agccagtcgc ggcttggtgc aggctgttta
     8401 ccggtgatga tttcgaccac cccaccgttg gtcagcggct ggttgagcgg cacgatgcgc
     8461 ccgtccactt tcgcgcctcg gcagcggtgc ccgagatcgg tatgcacgtg ataagcgaaa
     8521 tccagcgctg tcgcgccgcg tggcagatcc acgacctcgc cgcgcggcgt catggcgtaa
     8581 atgcgatcgg cgaacagttc gcccttcatg cgctcgatca ggtcgccttc gctgtgcgtc
     8641 tgctgcgtcg gatcgagaac ccggcgtacc cactcgatct tgcgttcgta gtccagatcg
     8701 cgggcgccgc cctccttgta acgccagtgc gcagccacac cgagttccgc gtactcgtgc
     8761 atatcctggg agcggatctg cacttcgagc gaccgcccct ccggaccggt caccgcggtg
     8821 tggatcgagc ggtactcgtt gtccttgggg gtggcgatgt agtcatcgaa ttcacccggt
     8881 atgtagtgcc acagcccgtg caccacgccg agcgcggcgt agcagtctga aaccgattcc
     8941 acgacgatgc gcaccgcgcg cacgtcatag agttgttcga atgcgatgtg cttgcgccgc
     9001 attttccgcg caatgctgta gatatgtttg ggtcgaccgt agaccgcgag cgcggtaacg
     9061 cccgcctgct cgagctcccg ccgcagcagc gcgcaaactt ctacgatata gcgctcgcga
     9121 tcgacgcggc gctccgccag ggcacgtgca atgcggtgat agtcctcggg ttccaggtag
     9181 cgaaaggcca gatcttccag ctcccatttg aggttccata cacccagacg attggccagc
     9241 ggcgccagca cttcccgcgt ttcgctggcg agacgccgct gttcttgcgg gggccggcca
     9301 cgagcgtgac gcagctggac caattgctcg gccagccggg cgaccaccag gcgcgggtcg
     9361 gcggccacgg ccagcagcat ttttcgcagg atctcggcct gttgcgcggt cagaccctgc
     9421 gacgccgacc acccggtagg caggtgaagc tcgcccaggc gctccagctc ggctgccagc
     9481 cgtgacggat tggcgcccaa acgggtctcg agctggcgga tatcgcgtgg cagtccggcc
     9541 gtgcgcgccc cgtgcaacag ggtgccgatt gccagatcgg tgtcgcgcgt cagggcggcc
     9601 accgtgtgag cagcctcggc gcccagatcc agcgccgccg gatcggcgcc atactcgcgc
     9661 agcaccgcgc gcgctgcatc gaggtgcgcg gcgatctcag gggcttcctg ggtcgcggtt
     9721 tccatgctgt gtggtcgatg ccttccggct atcattcagg gtatggcttg tgacagcaag
     9781 tgcagttgag gtagtacggt cgtggcgcga tatgcgggca agacttggcg ggcgttgcgg
     9841 ccgaccgata tacccactcg ggcgagctgc ctgccggtgc gcctgcacag tccggattgc
     9901 cgagtgctgg gactggatcc tggttcgcag cggaccggct atggtgtcat cgattgccag
     9961 gccggcgctg agcggcacct gaccagcggc tgtatcgacg tcactggaca ggacatggtg
    10021 gtgcggctgc aatgcatcta tcgctcggtg gtggcactgg tacacgagca tcgccccgac
    10081 atgatcgcga tcgagcgcgt attcgtacac cgcaatcccg atagcgcttt gaagctgggt
    10141 caggcgcggg gtgtggcgct gtgcgccgcg gcctctctgg gcgcgagcgt gcacgagtac
    10201 gccccgcgtg cgatcaagat ggcgatcacc ggctacggtg ccgccgccaa gccgcaggtc
    10261 gcgcagatgg tacgcaacct gctgatgctg gagatcaaac tcacgcccga cgcggccgac
    10321 gcgctggcgg tcgccttgtg tcactctcag tcgcgccgca tgggggcgct tgcggctgcg
    10381 gatgtcgcct gacagggccg gtaatcgatg atcggttcgc tctccggcgt gctcgtcgtc
    10441 aagtcgccgc cgcatttgct gctcgaggtc ggcggtgtcg gctatgaact cgaagcgccg
    10501 atgtctacct tttttgtgct gccggaagtc ggggagcgcg cacgtctgct gacacacctg
    10561 caggtgcgtg aggacgcgca cgtgctttac ggtttcagta ctgctgccga acgcacgctg
    10621 ttccgcaacc tgttgaaggt ttccggcgtg ggcccgcgga tcgcactggc tatcctgtcc
    10681 ggtgtctcgg cagcctcgtt tgcggaagca gtgcgtaacg aggacagcgc gatgctcacc
    10741 cgcattccgg gtgtcgggcg caagatcgcc gagcgtctga tcgtggagat gcgcgatcgc
    10801 atcgagagcg agtccgcctt ctcggtggcg ggcatggcga cgccgagcac gggcattccc
    10861 ttgccgatca tgggcggtgg tgcgcagcgt gaggccttca gtgcgctggt cgcgttcttc
    10921 ggttacaagc ccgcggaggc cagtcgtttg ctgaaagcag tggagcctga cagcggctcg
    10981 ctgagcaccg aagagtggat ccggcgtgcg ctgcaaagtg cggtgcgcgc gtgaatgccc
    11041 acgaggatga ccggctcgtc agcaccgcgg ccggcggcgg cgaggaactg attgatcgcg
    11101 cgatccgacc gcggctgctc aatgactaca tcggtcaggc cgccgtaaag gaacagctgt
    11161 cgattttcat cgacgcggcc cgcatgcgct ccgaggcgct cgatcacgtg ttgatcttcg
    11221 ggccaccggg cctcggcaag accacgctgg cgcatatcat cgcggccgaa ctgaaggtca
    11281 atctgcgaca gacctcgggt ccggtactgg agaaacccgg tgacctggcc gctatgttga
    11341 ccaaccttca gccgcgcgac ctgctgttcg tcgatgagat ccatcgactc agcccggtgg
    11401 tcgaggaaat cctctatcct gcgatggagg actatcagct cgacatcatg atcggggagg
    11461 gtccggccgc acgctcgatc aaattgtccc tgccgccctt caccctggtc ggtgcgacca
    11521 cgcgtgcggg gctgctgacc tcgccgctgc gcgatcgctt tgggattgtt cagcgcctgg
    11581 agttctacag tgtgtcggac attgagcgca tcgtgcatcg ctcggcgcgc atcctggaag
    11641 tggcgatcga gccggaagga gcgaagcgca tcgcgcagcg ctcacgcggt acgccgcgca
    11701 tcgccaaccg actgctgcgg cgtgtccgcg actacgcgca ggtcaagggt agcggacaca
    11761 tcgatgaggc gtgtgcgcat gccgcgctcg atctgctgca gatcgaccgc caaggcctgg
    11821 atgcgctcga tcacaagctg ttgcgcacga tcatggagaa gttcgacggc ggtccggtcg
    11881 gtatcgacag cctgtcagcg gcgatcggtg aggaacgcgg cacactcgag gatgtgatcg
    11941 aaccgttcct gatccagcag gggttcatca tgcgcaccgc acgggggcgg caggcgacgc
    12001 gtctggcctg gcagtatctg ggattgacgg tgcccgagcg cgcggtggcg aatctgtcgc
    12061 tgtttggtga agagtcttga gtgtgttcgc gtggcaggcg cgcgtctatt gggaagatac
    12121 ggacgctggc ggcatcgtct actatgggaa ttatctgcgt ttcctggaac gcgcacgcac
    12181 tgagtggctg cgcgccaagg gtgtctggca gcgcaagctc gaacaggagg ctggggtcat
    12241 cttcacggtg atcgacctgc aagtgaaata tcgtctgccc gcgcggctcg acgatctgtt
    12301 gtggatcagc tgcgagccgg cgcgcgaggg tggagcgacg attgcattcg cgcagcgcgt
    12361 ctggcgcgag cacgtcgggg gtgagctgct gctggaaggc gtggcg
//
LOCUS       DATMRM010000098        10913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3454263, whole genome shotgun sequence.
ACCESSION   DATMRM010000098 DATMRM010000000
VERSION     DATMRM010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10913
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3454263"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1605)
                     /locus_tag="VNZ06_02985"
     CDS             complement(205..1605)
                     /locus_tag="VNZ06_02985"
                     /inference="COORDINATES: protein motif:HMM:NF004767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWW19746.1"
                     /translation="MTDKPTNSLARADIATELHPYTDARLHEQTGPIVIERGNGIYVY
                     DNAGKEYIEAMAGLWSVGVGFSEPRLAQAAAAQMAKLPFYHLFGHKSHEPAIRLGEKL
                     VAMTPPQLTRVFFTNSGSEANDTVIKLIWFYNNALGRPKKKKFLARNKAYHGINVASG
                     SLTGLPNNHRDFDLPAIPVRHLTCPHFYRFGAVGESEAQFTARLLQETESVILEEGAD
                     SIAAFFGEPLMGAGGVMPPPQGYWPGIEALCRKYDILLVSDEVITGFGRLGSPFGAVH
                     YGFKPDVMVVSKQLTSSYVPLAAVLFTDGIYSAVADNTAKIGTFGHGFTTSGHPLATA
                     VALENLKILEERDLMGNAARMGVILQRELQQFASHPLVGEVRGAGLIAGIELVADKAT
                     RKPFAAQLKVGPRAFALGHQHGIIMRAIGDTLALCPPLIINEAQLHEVIVRVRKTLDA
                     TAAALAADGQAEKRYA"
     gene            complement(1645..3612)
                     /locus_tag="VNZ06_02990"
     CDS             complement(1645..3612)
                     /locus_tag="VNZ06_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWW19747.1"
                     /translation="MACATRAAPAQHAPSGVSAESPSVRQQFLAALQHVRVQAAEKPD
                     SEALIAYPIYDYLQVARLRRDLDAHPSEELDQTIDTFLQDRPGQPVTRVLRREWLANL
                     ATRGRWDWFLPRSTEVNDPVLVCDRLAGQLATGDTSVLGTDVLAVWSQATRQPHECDR
                     VFSWLRQQNLITPEAAEARARAALEADNSRLAREFAADVPPARAAALLLWVRLLESPK
                     GALEELGAHPEAPVEPEALIAGFTHLSRTDATTALNLLPLLLKRADLTAEVAGRLQRL
                     AALGAAYDHLPSAIDAFRALPKEAVDADVHEWRIRAALWATDYQTALTWITELPPTLA
                     SQPRWRYWNARATELTLGPEVAAPLFNEIAGLRDYYAYLAADHLHSTYSLNVHPTPDD
                     TEAQKTLSSAPGLIRAHELFECDLIEDASVEWSAVLADAEPATKIQAAHLASHWGWYT
                     QAITTLTQANEWDDVALRYPRPYPDIVAQASALTALPAEWILSVMRQESLFRQDATSR
                     ANARGLMQLQPATAVQVARRWHLAPPVHDALFDPAAAIPLGAAYLKEFVDKYHGEMAL
                     TLAAYNAGPWAVTRWLPSKATDADIWIENIPYNETRSYVQRVLEHIVAYSYQLDSGPP
                     RLVSLLAPVPSAADEPSLTRAALQGDPPARR"
     gene            complement(3635..6007)
                     /locus_tag="VNZ06_02995"
     CDS             complement(3635..6007)
                     /locus_tag="VNZ06_02995"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HWW19748.1"
                     /translation="MKAEPALSAELLRKMHAYWRAANYLSVGQIYLYDNPLLKRPLAT
                     GDVKHMLLGHWGTTPGQNFIYVHLNRVIRKHDLNMIYVSGPGHGGPAVVSNTYLEGTY
                     SEIYPDIGQDEAGLRKLFMQFSFPGGIPSHASPECPGSIHEGGELGYSLSHSFGAVFD
                     NPDLIVACVVGDGEAETGPLATSWHSNKFLNPVTDGAVLPILHLNGYKIANPTLLARI
                     DHAELEQLLRGCGWNPVFVEGHDPQPMHQAMAAALDHSIEEIRRIQQQARSRDDQSRP
                     RWPMIVLKSPKGWTGPANVDGEKVEGTFRSHQVPLLVSAAHPEHLGQLEGWLKSYHPE
                     ELFDERGRLRAELAELAPTGERRMGANPHANGGQLLRDLRMPDYREYGVEVPEPGVAG
                     IGDTHVLGRFLRDVVARNEEQRNFRIFGPDETLSNGLEAVFETTNRQWEASTDANDQY
                     LAPSGRVIEVLSEHQCEGWLEGYLLTGRHGLFNCYEAFIHIVDSMFNQHAKWLKVTAR
                     LPWRRKIASLNYLLSSHVWRQDHNGFTHQDPGFIDNVVNKKAEIVRVYLPPDANCLLS
                     VMDHCLRSRHYVNVIVAGKHPAPQWLGMPAAVRHCAEGIGIWQWASNDQDCEPDVVMA
                     CCGDVPTLETLAAVSILRQHLPDLRIRALNVVDLMKLQPKSEHPHGLSDTDFDALFTR
                     DRPVIFAFHGYPWLIHRLTYRRTNHHNLHVRGYKEEGTITTPFDMTVLNDLDRFHLVI
                     DTIDRVPKAGAAGNALRQRLQDKLVEHRQYINQNGQDMPEVRNWKWRTPA"
     gene            complement(6202..8256)
                     /locus_tag="VNZ06_03000"
     CDS             complement(6202..8256)
                     /locus_tag="VNZ06_03000"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HWW19749.1"
                     /translation="MMYGKSALVASAVLILLALQPGNAAAAPASSALASGIDTQYEDS
                     SIRPQDDIYKYLNGKWLASTQIPADKPGYGTFDKLFDESQDQLKQLVDVVSKNASAPA
                     GSDEAKIRDLYNSFMDEAHAESLGIKPLAPELARIDAIKSKQDIPELMGHLQRIGVTT
                     PFTPGVHLDNKDATKYVFDLGQDGLGLPDRDYYLKDDDAKLKEIRGKYQLHVAHALEM
                     LGDAQSRQEAAQILALETRLAKVQWTKVELRDPVKAYNKVELSALPTLAKGFDWHRYL
                     VTAGVAGKVSYVIVSQPSYQTGFAKILQDTPLPVWKVYFRWHLLSDASPYLSKAFVDD
                     RFAFYGTVLSGVPENRPRWKRGISLVDGSIGEGLGKLYVAKYFPPDRKARADELVRNL
                     LAAYKQDIGTLDWMTADTKQQAQAKLAKIMVKIGYPSKWRDYSTLTIRADDLLGNVQR
                     ANEFEWQRNINKLGQPVDRGEWGMNPQTVNAYYNPEMNEIVFPAAILQPPFFNAAADD
                     AVNYGAIGSVIGHEISHGFDDQGAQYDGDGNLRNWWTEEDHKRFDAKTKALVAEYGAF
                     EPVAGYHLNGELTLGENIADNSGLAIAYKAYKISLAGKPAPKIDGLSGDERFYLGFAQ
                     EWREKLRDNYAVQLIKTDPHSMGVDRVLGTLVNQPGFYETFNVKAGDKMYVPPQKRVV
                     IW"
     gene            8354..9013
                     /locus_tag="VNZ06_03005"
     CDS             8354..9013
                     /locus_tag="VNZ06_03005"
                     /inference="COORDINATES: protein motif:HMM:NF021141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HWW19750.1"
                     /translation="MRRLHSRAWMATFFLALALLGCRVPAPQRLSAEANERVYIVRRS
                     WHIDVGFAVAELQPPLASLRAAYPSARYLEFGFGDRHYLMTRHAGAGTLLSALWPGPG
                     LILMTALNASPQQAFGADNVLELRVSARQSEELQQFIWHTMTSDQDRVTAVAPGPYDG
                     SAFYPAIPKYSALHTCNTWAAESLHSAQLPIRSTGVEFAGQLWSQARRLQRMQASGTA
                     H"
     gene            complement(9043..>10913)
                     /locus_tag="VNZ06_03010"
     CDS             complement(9043..>10913)
                     /locus_tag="VNZ06_03010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWW19751.1"
                     /translation="SIAAAPAAQLDIYGHLPALEEVALSPDGSRVAFVKTQGDNRLIE
                     AWSLADRKLLGAARVGDQKLRAIRWADGDHLMVVTSAAGSMLGVTDLSEWSQLQIWDV
                     KTHRMRPVPQSDAYSGANMLNVIAGEPMVRQLHGHTSIFVPVITLSSARGPALERIDV
                     DGSEKQVTQGGEITRDWLVDSEGKITADMNYDEDRRRWSVRLNRNGSMQEVIAGKEAV
                     DVPQMLGFSPNGDSIVLQQLENNEWVWRPLSLKDGILGAPMPETQGLEAPIEQSQRMI
                     GGVSGSADGGYVFFDPGMQFRWDNVTKVFAGERVSLVSLDQDLLRFVVRVDGPTHGYV
                     YELVDMGAHKIEPLGDVYAGGIKPYEVRRITYAAADGMQIPAYLTLPRGRTEHDLPLI
                     VLPHGGPQVRDSADFDWWSQALADQGYAVLQPNYRGSDLSPKFVEAGYGEWGRKMQTD
                     LSDGVRYLAKQGFIDAKRVCIVGGSYGGYAALAGVTLDPGVYRCAVSVAGLSDLSLML
                     TWVEQRGGFRPQTVERYWDRFMGAKGPHDPALDAISPIRHIDAITAPVLLIHGKDDTV
                     VPYKQSQVMFDAMRQAKKNVQLVTLNHEDHWLSRGETRLQMLQASVAFLREHNPPD"
BASE COUNT         2026 a         3469 c         3260 g         2158 t
ORIGIN      
        1 cactgagacc gtaagcctca gggaacacca gcacgcccgg ccttttgccg ctcgcaggtt
       61 ccaggaacag ctgcgagtcc atcgacaaac catcggcctg atatttgaga tcgcgagtag
      121 gcataggcac tcccgagtag gagtgcaaga ttgtcacaag actacagccg cgtcagcccc
      181 ggccggccaa acatcgtggc gccctcaggc gtagcgcttt tccgcctgtc cgtccgccgc
      241 cagcgctgcc gccgtcgcat ccagcgtctt gcgcacgcga acaataacct catgcagctg
      301 cgcttcgttg atgataagcg gcgggcacag cgccagcgtg tcaccgatcg cgcgcatgat
      361 gatgccgtgc tgatgtccaa gcgcgaacgc tcggggcccg actttgagct gcgcagcaaa
      421 cggcttgcgg gttgctttgt ccgcaaccag ctcgataccg gctatcagtc ccgcgccacg
      481 cacttcgccg accagcggat ggctagcaaa ttgctgcaat tcacgctgca agatcacacc
      541 catgcgcgcc gcgttgccca tcagatcgcg ttcctcgaga atcttcagat tctccagcgc
      601 taccgcggtg gccagcggat gaccggaggt cgtgaatcca tggccgaacg tgccgatctt
      661 tgccgtgttg tcggcgaccg cggaataaat gccgtcggtg aacagcaccg cggccagtgg
      721 cacataggag gaggtcagct gcttggagac caccatgaca tccggcttga aaccgtagtg
      781 aaccgcaccg aatgggctgc cgagccgacc gaacccggtg atcacttcat ccgacaccaa
      841 caggatgtca tatttgcggc acagggcttc gatccccggc cagtagccct gcggcggcgg
      901 cattacgccg cccgctccca tcagcggttc gccgaagaac gcggcgatgc tgtccgcccc
      961 ttcctcgaga atgaccgact ccgtttcctg caacagtcgc gcagtgaact gcgcctcact
     1021 ctcaccgaca gcgccgaaac gatagaaatg cggacaggtc aggtggcgca ccggaattgc
     1081 cggcagatcg aaatcgcgat gattgttggg cagtccggtc agactgccgg atgcgacatt
     1141 gatgccgtga taggccttgt tgcgcgcgag gaattttttc ttcttcgggc gccccaatgc
     1201 gttgttgtag aaccagatca gcttgatgac ggtgtcgttc gcttcagagc cggagttggt
     1261 gaagaacacg cgtgtcaatt gcggcggtgt catcgcgacc agcttctcgc ccaggcgtat
     1321 cgccggctcg tgtgatttgt ggccgaacag gtgatagaag ggcagcttcg ccatctgcgc
     1381 ggcagcggcc tgagccagtc gaggctcgct gaagcctacg ccgaccgacc acaatcccgc
     1441 catcgcctcg atgtattcct tgccggcatt gtcatagacg tagatgccgt tgccgcgctc
     1501 gatcacgatc ggtccggtct gctcgtgcag gcgagcgtcc gtgtaaggat gcagctcggt
     1561 ggcgatatcc gcacgggcca atgaattggt cggtttgtcg gtcatggcgc gcgtccgttt
     1621 gatctgggtg cggccgggat tctatcagcg ccgcgcgggc gggtccccct ggagcgccgc
     1681 ccgggtcagg gacggctcat cggcggcgga aggcaccggg gcgagcagcg aaaccaggcg
     1741 tggcggcccg gaatcgagct ggtatgaata ggccacgatg tgctcgagca ctctctgcac
     1801 gtagcttcgg gtttcgttgt aaggaatatt ctcgatccag atatcggcgt cggtcgcttt
     1861 gctcggcaac cagcgcgtca cggcccaggg cccggcgttg taggcggcca gcgtcagcgc
     1921 catctcaccg tggtatttgt cgacgaattc cttcaggtag gcggcgccca gcggaatggc
     1981 cgcggccgga tcgaacagcg catcgtgtac cggcggtgct agatgccagc gacgcgcgac
     2041 ctgaaccgcg gttgcgggtt gcaactgcat caatccacgg gcgttggcgc gggaggtcgc
     2101 gtcctggcga aacagacttt cctgacgcat gaccgaaagt atccattctg cgggcaacgc
     2161 ggtcagcgcg ctcgcctgcg ccacgatgtc gggatacggg cgcggatacc gcagtgccac
     2221 gtcatcccac tcgttcgcct gtgtcaatgt cgtgatcgcc tgcgtgtacc aaccccagtg
     2281 cgacgccaga tgtgccgcct gaatcttggt cgccggttca gcatcggcca gcaccgccga
     2341 ccactcgacc gacgcgtctt cgatcagatc acactcgaat agttcatggg cgcgaatcag
     2401 acccggagcg ctgctgagcg tcttctgggc ctcggtatca tccggtgtcg gatgcacgtt
     2461 gagactgtag gtgctgtgca gatgatccgc cgcaaggtac gcgtaatagt cgcgcaggcc
     2521 cgcgatctcg ttgaacaacg gtgcggccac ctcgggtcct aaggtgagct cggtggcccg
     2581 cgcattccag tagcgccagc gcggctgcga agcgagcgtt ggcggcagct cggtgatcca
     2641 ggtgagcgct gtctgatagt cagtggccca cagcgcagcg cgaatccgcc attcatgcac
     2701 gtctgcatcg acggcctctt tggggagcgc gcgaaaggca tcgatcgccg agggcaaatg
     2761 atcgtaggcg gcaccgagcg cggccagacg ttgcagtcga cccgccacct ctgccgtcag
     2821 gtcggcgcgc ttgagcaata aaggcagtag gttcagtgcc gtggtcgcat cggtgcgcga
     2881 cagatgggtg aagccggcaa tgagagcctc gggctcgacc ggcgcctccg gatgcgcgcc
     2941 gagctcttcc agcgctcctt tcggtgactc cagaagcctc acccacagca gcaacgccgc
     3001 ggcgcgcgcg ggcggaacat cggccgcgaa ttcacgcgcc aggcgggaat tgtcggcctc
     3061 cagcgcagcg cgcgcgcggg cctcagcagc ctccggcgta atcaggttct gctgccgcag
     3121 ccacgaaaac acacggtcgc actcgtgcgg ctgacgcgtg gcctgagacc acacggccag
     3181 gacatccgtg ccgagcacgg atgtgtctcc ggtcgctaat tggccggcca gacggtcgca
     3241 gaccagtacc ggatcattga cctcggtgct acgcggcaga aaccagtccc agcggccgcg
     3301 ggttgcaagg ttcgcaagcc attctcgtcg cagcacgcgc gtaaccggct ggccgggtcg
     3361 gtcctgcagg aacgtatcga tcgtctggtc aagttcctcg gacgggtggg catccagatc
     3421 gcggcgcagc cgcgcgacct gcaggtagtc gtagatcgga tacgcgatca acgcttctga
     3481 atcgggcttc tccgcagcct gcacccgcac atgctgcagc gctgccagga actgctggcg
     3541 caccgaaggg ctctccgcac tgactccgga gggtgcgtgt tgcgccggcg cagcgcgcgt
     3601 cgcgcaggcc acgatcagta gtgcacaggc gcgcctaagc aggtgtcctc cacttccaat
     3661 ttcgcacttc gggcatgtcc tggccgttct gattgatgta ttgccgatgt tccaccagtt
     3721 tgtcctgtag ccgttgacgc aaagcgttgc ctgcggcccc cgctttgggc acgcgatcga
     3781 tggtgtcgat caccagatga aacctgtcca gatcattcag caccgtcatg tcgaacggcg
     3841 tggtaatggt gccctcctcc ttgtaaccgc gcacgtgcag attgtggtga ttggtgcgtc
     3901 ggtatgtgag ccgatgtatc agccaggggt agccatggaa cgcaaaaatg accggccggt
     3961 cccgggtgaa cagtgcgtca aagtccgtgt cgctcagacc gtgtggatgt tcgctcttgg
     4021 gttgcagttt catcagatca accacgttga gcgcgcgaat tcgcaggtca ggcagatgtt
     4081 gtcgcaggat cgagacggcc gccagcgtct caagcgttgg cacatcgccg cagcacgcca
     4141 tcacgacgtc cggctcgcag tcctggtcgt tgctggccca ctgccagatg ccgatgcctt
     4201 cggcgcaatg cctgaccgcc gccggcatgc cgagccactg aggcgccggg tgtttgcccg
     4261 ctacgatcac attgacgtag tggcgactgc gcaagcagtg atccattact gacagcagac
     4321 agttcgcatc gggcggcaga tagacccgca cgatctcggc tttcttgttg accacattgt
     4381 cgatgaaccc cggatcctga tgcgtaaagc cgttgtgatc ctggcgccag acatgtgatg
     4441 acagcagata gttgagcgaa gcgatcttgc ggcgccatgg caggcgcgcg gtcaccttga
     4501 gccacttcgc gtgctgattg aacatcgagt cgacgatgtg gatgaaggct tcatagcagt
     4561 tgaacagtcc gtgccggccg gtcagcagat acccctcgag ccagccctcg cactggtgtt
     4621 cactcaggac ctcgatcacg cgtccgctcg gcgcgagata ctgatcgttg gcatcggtgg
     4681 acgcttccca ctggcggttg gtggtctcga atacggcctc cagaccgttc gagagcgtct
     4741 cgtcgggacc gaagatgcgg aagttgcgct gctcctcgtt gcgcgcgacc acatcacgca
     4801 gaaaacgacc cagcacatgc gtatcgccaa tgccagcgac tcctggctca ggtacctcca
     4861 cgccatattc gcggtagtca ggcattcgca gatcgcgcaa caactggcca ccgttggcgt
     4921 gcggattggc gcccatgcgc cgttcgcccg tgggcgccag ttccgcaagc tccgcgcgca
     4981 agcgaccgcg ttcgtcaaac aattcctcgg ggtgatagct cttcagccac ccttccaact
     5041 ggcccaggtg ttccggatgc gcggcactga ccaatagcgg cacctggtgc gaacggaacg
     5101 taccctccac tttctcgccg tcaacgttcg caggtccggt ccagcccttc ggcgacttca
     5161 atacgatcat cggccatcgc ggccgtgact ggtcatcacg gctgcgcgct tgctgctgaa
     5221 tgcgtcgaat ctcctcgatc gagtgatcca gcgcggccgc catcgcctga tgcatcggtt
     5281 gcggatcgtg accttcgacg aacaccggat tccagccgca gccgcgcaac aactgttcga
     5341 gctccgcgtg atcgattcgc gccagcaagg tcggattggc gattttgtag ccgttcagat
     5401 gcaggatcgg cagcactgcg ccgtccgtga ccggattcag aaatttgttc gagtgccacg
     5461 aggtcgcgag cggcccggtc tccgcctctc catcgcccac tacgcaggca acgatcaggt
     5521 ccggattgtc gaataccgcg ccgaatgaat ggctgagcga atagcccagt tcaccgcctt
     5581 cgtgaatcga gccgggacat tcgggtgacg catgactcgg aattcctccc ggaaaggaga
     5641 actgcatgaa cagcttgcgc aacccggcct cgtcctggcc gatgtcagga tagatttcgc
     5701 tgtaggtgcc ttccagataa gtgttgctga ccaccgccgg accaccatga cctggtccgg
     5761 aaacatagat catgttgagg tcgtgcttgc ggatgacacg gttcagatgc acatagatga
     5821 agttctggcc aggggtcgtc ccccagtgcc cgagcagcat gtgcttcacg tcaccggtcg
     5881 cgagcggacg cttcagcagc ggattgtcgt ataaatagat ctgcccgacc gacagatagt
     5941 tggccgcgcg ccagtaggca tgcattttgc gcagcagctc tgcgctcaaa gcgggttctg
     6001 ccttcatgat cactccggca ggcgagggga gcgatcagtg tattagaaat tgcatgccga
     6061 cgtagctgat gaattcgatc tgtccgtcag cggcgccgtt gcgagcgtgg cccgcccggg
     6121 gtggaacggt gacccggcct gcgctcgtgc gagcgcaggc cacagtccgc cgcccatcga
     6181 acaaaaatgc aacagccagc ctcaccaaat gaccacgcgc ttctgcggtg gcacgtacat
     6241 cttgtcgccc gcctttacat tgaaggtctc ataaaagccc ggctgattga ccagcgtgcc
     6301 cagcacccgg tccacgccca tcgaatgcgg atcggtcttg atgagctgca cggcgtaatt
     6361 atcccgcagc ttctcgcgcc actcctgggc aaagcctaga tagaaccgct catcaccgct
     6421 cagaccgtcg atcttgggcg cgggcttacc cgccagtgag atcttatagg ccttgtacgc
     6481 gatcgcaagc cccgagttat cggcgatgtt ctcacccagt gtgagctcgc cgttgaggtg
     6541 atagccggct accggctcga atgcgccgta ctcggcgacc agcgccttgg tcttggcgtc
     6601 aaatcgcttg tggtcttcct cggtccacca attgcgaaga tttccatcgc cgtcgtactg
     6661 cgcgccctga tcgtcaaagc cgtggctgat ctcgtgaccg atgaccgagc cgatcgcacc
     6721 atagttgacc gcgtcatccg ctgccgcgtt gaagaacggc ggctgcagaa tcgcagccgg
     6781 gaacacgatc tcattcattt ccgggttgta atacgcgttc accgtctgcg ggttcatgcc
     6841 ccactcgccg cgatccaccg gttgccccag cttgttgatg ttgcgctgcc attcgaactc
     6901 gttcgcccgc tggacgttgc cgagcaggtc gtccgcacga atcgtgagtg tcgagtagtc
     6961 gcgccacttc gacggatatc cgatcttcac catgatcttg gcgagcttgg cttgagcctg
     7021 ctgcttggta tccgcggtca tccagtccag agtgccgata tcctgcttgt aggcagcgag
     7081 cagatttcgt accagctcat cggcgcgcgc ttttcgatcg ggcggaaaat acttggctac
     7141 gtagagcttg cccagacctt cgccgatcga gccgtccacc agcgagatgc cgcgcttcca
     7201 gcgcggccga ttctcgggca cgcccgatag cacggtgccg taaaacgcga agcgatcatc
     7261 aacgaaggct ttggacagat acggcgacgc gtcggacagc agatgccatc ggaaatagac
     7321 cttccagacc ggcagcggcg tgtcctgcag aatcttcgcg aagccagtct ggtagctcgg
     7381 ctggctgacg atgacgtagc tgaccttgcc cgctacaccg gctgtcacca ggtagcggtg
     7441 ccagtcgaaa cccttggcca gtgtgggcag cgccgacagc tcaactttgt tgtaggcctt
     7501 gaccggatcg cgcagctcca ccttggtcca ctgtaccttc gccaggcggg tctccagtgc
     7561 caggatctgt gcggcttcct gcctggactg cgcatcgccc agcatttcca gggcgtgcgc
     7621 cacgtgcagc tggtacttgc cgcgaatctc tttcagcttc gcatcgtcat ccttcaggta
     7681 gtagtcgcga tcaggcaatc cgaggccgtc ctgccccaga tcgaacacat acttggtcgc
     7741 atccttgtta tccagatgca cgcctggcgt aaacggcgtg gtcacgccga tgcgctgcag
     7801 atggcccatc agttccggaa tgtcctgctt gctcttgatg gcatcgatgc gagccagctc
     7861 cggcgccaac ggcttgatgc cgagtgattc ggcatgcgcc tcgtccatga agctgttgta
     7921 gaggtcgcgg atcttcgcct cgtcgctacc ggccggcgcg ctggcattct tggataccac
     7981 gtcaacgagt tgcttcaact gatcctgcga ttcatcgaac agcttgtcga aagtaccgta
     8041 ccccggctta tcggccggaa tctgggtgct cgccagccac ttaccattga gatatttgta
     8101 gatgtcatcc tgcggacgga tgctcgaatc ctcgtactgc gtatcgatcc cagatgcgag
     8161 cgcgctggat gcgggcgcag cagcagcgtt tcctggctgt agcgctaaaa gaatcagcac
     8221 ggcgctggcg accagcgcgg acttcccgta catcatctgt ttctcctgct gaagcgttgt
     8281 gagggcgggt ggcaattcta ttgcggcgcg aacggcatcg ctatacttca ggcccattcg
     8341 ctctgctgcc gccatgcgac gactccactc cagagcctgg atggccacgt tctttttggc
     8401 gctcgcgctc ctgggatgtc gcgttccagc accacagcga ctgagtgccg aagcgaatga
     8461 gagggtctac atcgtcagac gttcctggca catcgacgtc ggtttcgcgg tagcggagtt
     8521 gcagccgccg ctggcgagcc tgcgggcggc gtatccttca gcgcggtacc tcgaattcgg
     8581 gttcggcgac cgccactacc tgatgacgcg acatgctggc gccggaacct tgctctcggc
     8641 cctgtggccc ggccccggcc tcattctgat gaccgcattg aacgcgagtc cgcagcaggc
     8701 attcggtgcc gacaacgtgc tcgagctgcg agtctccgcc agacaatccg aggagcttca
     8761 gcagttcatt tggcacacga tgacgagcga tcaggatcgc gtcacagcgg ttgcccccgg
     8821 gccgtatgac ggtagtgcct tctacccggc gatccccaag tattcagcgc tgcatacgtg
     8881 caacacctgg gctgcggagt ctttgcattc cgcgcagttg ccgatccgca gcacaggcgt
     8941 cgaatttgcc ggtcagctgt ggtctcaggc ccggcgtctg cagaggatgc aggctagcgg
     9001 tacggcccac tagagccgtg ggaacagacc acggggggcg ctctagtcgg gcgggttgtg
     9061 ctcgcgcaga aaggccaccg aggcctgcag catctgtagt cgcgtctcac cgcgcgacag
     9121 ccagtgatct tcgtgattga gcgtcaccag ttgaacattc tttttggcct ggcgcatggc
     9181 atcgaacatg acttggctct gcttgtaggg tacgaccgta tcgtccttgc cgtgaatcaa
     9241 caacaccggg gcagtgatgg catcgatatg cctgatcggt gagatggcat ccagtgcggg
     9301 atcgtgcggt cctttcgcgc ccatgaaccg gtcccagtag cgctctaccg tctgtggacg
     9361 aaatccgccg cgctgctcca cccaggtgag catcagcgac agatccgaca gtcccgccac
     9421 ggacaccgcg cagcgataga cccccggatc gagcgtgacg cccgcgagcg cggcgtagcc
     9481 accatagctg ccgccaacga tgcagacgcg ctttgcatcg atgaatccct gtttggccag
     9541 atagcgcacg ccatccgaca ggtcggtctg catcttgcgg ccccactcac cgtatcccgc
     9601 ttcaacgaat ttcggcgaca gatcggatcc gcggtagttg ggctgcagca ctgcgtaccc
     9661 ctggtcggcc agtgcctgcg accaccaatc gaaatccgcg ctgtcgcgta cctgcggacc
     9721 gccatgcggc agtacgatta acggcagatc atgttccgtg cgtccgcgcg gcagggtcag
     9781 atacgccgga atctgcatgc cgtccgcagc ggcataggtg atgcgccgaa cctcgtaagg
     9841 cttgatgcca ccggcataga catcccccag cggctcaatc ttgtgcgccc ccatgtccac
     9901 cagttcgtaa acatacccat gggtcggtcc atccacgcgc acgacgaacc tcaaaagatc
     9961 ctgatcgagt gacaccaggc tcacgcgctc gccagcgaaa accttcgtga cgttgtccca
    10021 gcgaaactgc atgccgggat cgaagaagac gtatcccccg tcggccgagc cgctcactcc
    10081 gccgatcatt cgttgcgatt gctcgatcgg cgcttcgagc ccttgcgttt ccggcatcgg
    10141 tgcgcccagg atgccgtcct tcagcgacaa cgggcgccag acccactcgt tgttctccaa
    10201 ctgctgcagg acgatcgagt cgccgttcgg actgaaaccc aacatctgcg gcacatcgac
    10261 cgcctccttg ccggcgatca cttcctgcat gctaccgtta cgattgagtc gaaccgacca
    10321 gcgacgacga tcttcgtcgt agttcatgtc cgcggtgatc ttgccttcgg agtcgaccag
    10381 ccagtcccgg gtaatctcgc ctccctgagt cacctgtttc tcgctgccat cgacgtcgat
    10441 gcgctccagc gcaggtccgc gcgcgctaga cagcgtgatc actggcacaa agatcgatgt
    10501 atgcccgtgc aactgccgca ccatcggttc gccggcaatc acattcagca tgttggcacc
    10561 cgagtacgcg tccgattgcg ggactggtcg catccgatgc gttttaacat cccagatctg
    10621 aagctggctc cactcactca gatccgtcac ccccagcatc gaccctgcag cagaggtcac
    10681 aaccatcaga tgatcgccgt ccgcccatcg aatggcccgc agcttctgat caccgacgcg
    10741 cgcggcgccg agcagtttcc ggtccgccag ggaccaggct tcgatcaggc gattgtcgcc
    10801 ttgagtcttg acgaatgcga cacgcgagcc atcggggctg agggctactt cctctagcgc
    10861 cggcaaatgg ccatagatgt caagctgagc tgccggggca gcagcgatcg acg
//
LOCUS       DATMRM010000099         3845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2533586, whole genome shotgun sequence.
ACCESSION   DATMRM010000099 DATMRM010000000
VERSION     DATMRM010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2533586"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VNZ06_03015"
     CDS             complement(<1..777)
                     /locus_tag="VNZ06_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19752.1"
                     /translation="MPTIRERVDRKGKKHFHAQVRMAGFPPRTASFPTRRQAERWATT
                     VEAEMIDGKHFRSAEARRRTLGQAIDRYLEEEVPKKRDGSMSRVNLPWWKKKLGYLKL
                     ADVTPELIVEYRGVLAKEPYRRADPTAAHTSLKKGTTAAEFKRRNATVNRYLTAASHV
                     FTVARKEWRWTSVNPFDSVSKLPEGRGRIRYLSDDERARLLKATAGNATLHLFTVVAL
                     STAARAGELLKLCWPDVELRPEDKEAKPPVPAEGRLLFRVT"
     gene            complement(906..980)
                     /locus_tag="VNZ06_03020"
     tRNA            complement(906..980)
                     /locus_tag="VNZ06_03020"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(946..948),aa:Thr,seq:tgt)
     gene            complement(1028..1690)
                     /locus_tag="VNZ06_03025"
     CDS             complement(1028..1690)
                     /locus_tag="VNZ06_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19753.1"
                     /translation="MRNVFLLLLFINVVFFGWSRWVDRPRDGAAVAATRSAAAVPALE
                     LTQAPVKPAAPPEPTTHCRSIGPFVDAAAAAPTADALRARGLLPRPRSVDTTTPDGYW
                     VYVEDLKDAAARRKVISTLNANGIKDAAAMADEAERVSVGVFADQRHAVHRAEQVQEL
                     GFKPVLSLHQKTISVDWLDVELKPNDADPTAIATGPTMSPKAAGPEPVRVVDCPAKPA
                     TG"
     gene            complement(1711..2538)
                     /locus_tag="VNZ06_03030"
     CDS             complement(1711..2538)
                     /locus_tag="VNZ06_03030"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HWW19754.1"
                     /translation="MSLLLLDSGNSRLKWALSREPYRRRQAFAARGAFDLNALRGSRS
                     PLDRLFKALRASGLTPRVQGCNVAGAAVERQLRAATRRAGFGPIRLAESHVAECGVRN
                     GYREVWRLGADRWVALVGARHEHPNMDLCIVGIGTAMTIDLLDSSGRHVGGNIIPGPK
                     LMIESLLTRTAGIRRRAGGSSAMSNIDLASSPDRPLSLFAHDTRSGLRAGARHACAAA
                     IEHARSAAGLELGRRPRLLLAGGAMDVIAPLLDSPYWREDDLILRGLAVIASTAVGA"
     gene            complement(2535..3533)
                     /locus_tag="VNZ06_03035"
     CDS             complement(2535..3533)
                     /locus_tag="VNZ06_03035"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HWW19755.1"
                     /translation="MSERESLPQRVCRLLSDGQFHSGTALASSCGVSRNAIWKAVAGL
                     RALGLNVHAVANRGYRIPNASDLLDPALILSSLPKEVAGRLRQGQCVWRTASTNLDLL
                     ARAPGPVGSFDYLTAECQVQGRGRRSRSWFAPPCGAICLSMSWNFAALPADASALSLA
                     VGVCVLRALKHVGLSGLALKWPNDLVVNGEKLAGLLIELRAEAGGPAYVVVGIGLNVV
                     LGDAVHRQIEAAGNRAADLLALGIQRADRNEIVAALIASTAAGLEQYERDGFSSFAPE
                     WREADALAGKVVVVSSEAGSVTGHARGIDLDGALCLQTRDGLQRFVTGDVSVRAVT"
     gene            complement(3530..>3845)
                     /locus_tag="VNZ06_03040"
     CDS             complement(3530..>3845)
                     /locus_tag="VNZ06_03040"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HWW19756.1"
                     /translation="EGDRARVLQCLRATREVIEISMPLMAQFAANIMELRAPRPKAQA
                     ARILVLSQRARAAFERSDADAWQRLRGCVDQVLAVPVPTIENVGGGGVRCMLAEVPEV
                     TT"
BASE COUNT          681 a         1336 c         1163 g          665 t
ORIGIN      
        1 cgtgacgcgg aagagcaacc gcccttctgc cggcaccggc ggttttgcct ccttgtcttc
       61 aggcctcagc tcgacatccg gccagcacag cttcaggagc tcgcccgcgc gcgctgcggt
      121 cgatagagcc actaccgtga acaagtgcag tgtcgcattg cctgccgtcg ccttgagcag
      181 cctggcgcgc tcatcgtcgc tcaggtatcg gattcggccg cggccttccg gcagtttcga
      241 caccgagtcg aaggggttca cgctggtcca gcgccactcc ttgcgggcca ccgtgaacac
      301 atggctggcg gccgtcaggt accggttgac cgtggcgttg cggcgtttga attcggcagc
      361 cgtggtgccc ttcttcaggg aggtgtgcgc cgcggtaggg tctgcgcgcc ggtaaggctc
      421 cttggccagg acgccgcggt attcgacaat cagctcgggg gtgacatcgg ccagcttcag
      481 gtaaccgagt ttcttcttcc accagggcag gttcacacgg ctcatggatc cgtcgcgctt
      541 cttcggcacc tcctcctcga ggtacctgtc gatcgcctgc cctagggtcc ggcggcgcgc
      601 ctcggccgat cggaagtgct tcccgtcaat catctcggct tccacggtcg tcgcccagcg
      661 ctcagcctgg cgcctggtag gaaagctggc ggtgcgaggc gggaaaccgg ccatccgcac
      721 ctgcgcgtgg aagtgcttct ttcccttcct gtcgacgcgt tcgcgaatcg tgggcatgcc
      781 tggcaacacc gtttcacacc agattgcgcg actgtacgcc acaggcgcat actgtgccat
      841 agctgtgcca cggcacatgg gtgcagccga cgctggtctg taagttgttg ttattagtag
      901 attgttggtg ccggcagaga gatttgaact cccgaccaac ggtttacaaa accgctgctc
      961 taccactgag ctatgccggc atattcgcgc ggggcgcgta tgttaccgca gcctcgagcg
     1021 ccctccctca gcccgtggca ggttttgccg gacagtccac gacacgcact ggctcggggc
     1081 ctgcggcctt gggactcatg gtcggccccg tggcgatcgc cgtcggatca gcatcgttcg
     1141 gcttcagctc cacatccagc cagtcgacgc tgattgtctt ctgatgcagg ctcaacaccg
     1201 gcttgaagcc tagttcctgt acctgttcgg cccggtgcac ggcatggcgc tgatcggcga
     1261 acactccgac cgagacgcgc tcggcttcat cggccatcgc cgcagcatcc ttaatgccgt
     1321 tggcgttgag cgttgaaatc actttgcggc gagccgccgc atccttgagg tcctcgacat
     1381 acacccagta tccatccggt gtggtggtat ccacgctgcg gggccgcggc agtaacccgc
     1441 gcgcacgcaa cgcatcggcg gtcggcgctg ccgccgcggc atcgacgaac ggaccaatgc
     1501 tgcggcagtg tgtggtcggc tccggcggcg ccgcgggttt gacgggcgcc tgtgtcagct
     1561 cgagtgcagg tactgccgca gcgctgcggg tcgctgctac ggccgcgccg tcgcgtggcc
     1621 gatcgaccca gcgcgaccag ccgaagaaca cgacattgat gaacagtaac agcaggaaga
     1681 cgtttcgcac gcgagcgagt gtatgccatg tcacgcgccc acagcagtcg aggcaatcac
     1741 cgccagaccg cgcagaatca gatcgtcctc gcgccaatac gggctgtcga gcagcggcgc
     1801 aatcacgtcc attgcaccgc ccgccagcag cagacgcggc cgacgaccca gttcaagccc
     1861 agcagcgctg cgcgcatgct caatcgcggc ggcgcaggca tggcgcgccc cggcccgcag
     1921 gccgctgcgc gtgtcatgtg cgaacagtga cagtggcctg tcgggagatg aagccagatc
     1981 aatattgctc atcgccgagc tgccgcccgc gcgccgccgg atgccagccg tacgcgtcag
     2041 cagcgattca atcatcaatt tcggaccagg aatgatattg ccgccgacat gccggccgct
     2101 ggagtcgagc aaatcgatcg tcatcgcagt accgataccg acgatgcaaa gatccatatt
     2161 gggatgttcg tggcgggcac cgacgagcgc cacccaacga tccgccccca aacgccacac
     2221 ctcccgatag ccgttgcgca caccgcattc ggcaacgtgg ctttcggcaa ggcggatcgg
     2281 gccgaatccg gcgcgtcgtg tggccgcgcg caactgccgc tcgaccgcgg ccccggcgac
     2341 attgcagccc tgcacccgcg gggtgagccc cgaggcacgc agtgccttga acagcctgtc
     2401 caacggtgat ctcgatccgc gcagtgcgtt cagatcgaac gcaccgcgag ctgcgaaggc
     2461 ctgtcgacgt cgatacggct cgcgcgacag cgcccatttg agacgcgaat tgccactgtc
     2521 cagcagcaac aggctcatgt cacggctctg actgacacgt ccccggtcac gaaacgttgc
     2581 aggccatcgc gcgtctgcag gcatagcgcg ccatccagat cgatgccgcg cgcatgcccg
     2641 gtaacgctac ccgcttcgga actgaccacc accaccttgc cggccagagc atccgcctcg
     2701 cgccactccg gcgcaaagct gctgaacccg tcgcgctcgt actgctcgag ccccgcagcc
     2761 gtgctggcga tcaaggcggc cacgatttcg ttgcggtcgg cgcgctgaat ccccagcgcc
     2821 agcaggtccg ctgcacgatt gcccgcagct tcaatctgac gatgcaccgc atccccgagc
     2881 acgacattca ggccgatgcc gacgacgaca tacgccggcc cccccgcctc cgcacgcaac
     2941 tcgatcagaa gaccggccag tttctcgccg ttcacgacca ggtcgttcgg ccacttgagt
     3001 gccagacccg atagaccgac gtgcttcaac gcacgcaaca cgcagacgcc gaccgccaga
     3061 ctcagcgcgc tcgcatccgc gggcagcgct gcgaagttcc agctcatgga caggcagatc
     3121 gcaccgcagg gtggggcgaa ccagctgcgc gagcggcgcc cgcgtccctg gacctgacat
     3181 tcggccgtca gataatcgaa acttccgacc ggtccgggcg cgcgtgcgag cagatccaga
     3241 ttggtggaag cggtacgcca gacgcactgg ccctgtcgca gcctgcctgc gacctctttc
     3301 ggcaaagagc tcaggatcag tgcggggtcg agcagatcac tggcatttgg gatccgatag
     3361 ccgcggttgg cgacggcatg cacgttcagc ccgagtgccc gcaggcccgc caccgctttc
     3421 cagatcgcat tgcgactgac cccgcaactg ctcgccagcg cggttccgga atggaattga
     3481 ccatcagaca gcagccggca gacccgctgc ggcagagatt cgcgttcgct cacgtcgtga
     3541 cttccggcac ttccgccaac atacaccgaa ctcccccgcc accgacgttt tcaatagtgg
     3601 gcaccggcac cgccagcacc tggtcgacgc agccgcgcag acgttgccac gcatcggcgt
     3661 cggaccgttc gaacgcggct cgtgcccgct gtgacaacac cagtattcgc gcagcctgtg
     3721 ccttgggccg gggcgcacgc agctccataa tgttggcggc gaactgcgcc atcaacggca
     3781 tcgatatttc gatgacctcg cgcgtcgcgc gcagacactg caacacccgc gcacgatcgc
     3841 cctct
//
LOCUS       DATMRM010000100         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3455441, whole genome shotgun sequence.
ACCESSION   DATMRM010000100 DATMRM010000000
VERSION     DATMRM010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3455441"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..>2022
                     /locus_tag="VNZ06_03045"
     CDS             230..>2022
                     /locus_tag="VNZ06_03045"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWW19757.1"
                     /translation="MRIARGWMGVFLALCAGGSASAGESSPLTPAQALSYVWVGDLHF
                     SPDGSKLAFVVGSYKWDALPHIRISDIASGATREITPSGRSERTPQWSADGKTLAFLS
                     NRGGGRAQVFLLPMSGGEAKALTASRFGVRRFRWSPDGRGIAYLAQEDSARDESDGPQ
                     LADDESNLARVWFVDVSSRKTRRISSSSAYRIDDFQWQGPSSLLVSATDRPRVEEYTD
                     RVYRLALADGLFQSVGSPPQPFNTLLVSPDATQYAVRSTNSQGPRARDLFVATLPEGE
                     LRDISAPLGLTIAEVRWHEQDVVWVRAIDGFYNRIFRIRRGESPQRIDLPLSAAAFDV
                     SNQGVLAYVGQDYQHLPEIYLRGADGSLRQMTHLQQGWEGISLASSTIFRTVTADNLS
                     IEAALLQSPSSVDGHKRPLVLLVHGGPPSNFSAGYDWEEAWAQLLVSRGYQVLMVNPR
                     GSDGYSEAFLKANRADWGGGDYRDLMSVLDAVVARGSTDAARIGIGGWSYGGEMAAWA
                     ITQTDRFKAAVAGACVFDQAGEFETEEDDPAADEWYFGTPWEHPDVFARNSPVTFIGQ
                     AHTPTLILDGEDDANNPVGQSKGLYRALKHLG"
BASE COUNT          363 a          614 c          667 g          378 t
ORIGIN      
        1 tcggtgcggc cgctggctgt gctgtcggcc atcatttggc gaagaagaag cagcaggaac
       61 agcaacagcc gctgcagcag ccttcgcagc aacccccgcc cgctccgccc tcatcgcaag
      121 gaggcgctcc cggcagtagc agccaggcga tctaaccaag tcgcgcccgc gcgccttgac
      181 gattcgcgct gcaggtatcc tgcacgcctc gaaccaggga ggcaggtggg tgcgaatcgc
      241 gcgaggttgg atgggtgtct ttctcgccct gtgtgctggc ggctctgcct cggcgggcga
      301 atcgtcgccg ctgactccgg cacaagcatt gtcttacgtc tgggtcggcg acctgcattt
      361 cagccccgac ggctcgaagc tcgccttcgt ggtgggcagt tacaagtggg acgcgctgcc
      421 gcacattcgt atcagcgaca ttgcaagcgg cgcgacgcgt gagatcacgc cttccggcag
      481 atcagagcgc actccgcaat ggtctgcgga cggcaagacg ctggcgttcc tgtccaatcg
      541 cggtggcggt cgggcgcagg tctttctctt gccgatgtcg gggggcgaag ccaaggcgct
      601 gacggccagc cggttcggcg tgcgccggtt tcgctggtca ccggatggac gcggcattgc
      661 ctatcttgcg caggaggaca gcgcgcgtga tgaaagcgat ggcccgcagc tcgccgatga
      721 cgaaagcaat ctggcaagag tgtggttcgt cgatgtgagc tcaagaaaga ctcgcagaat
      781 cagcagcagc tcggcctatc gcattgatga tttccagtgg caggggccga gcagcctgct
      841 cgtcagcgcc acggatcggc cacgtgtcga ggaatatacc gaccgtgtct accggcttgc
      901 gctggcggac gggctgtttc aaagcgtggg cagcccgcct caacccttca atacgctgct
      961 ggtctcgccc gatgcaaccc aatatgccgt gcgctcgacg aactcgcagg ggccgcgtgc
     1021 ccgcgacctg ttcgtggcga cgctgcccga aggtgagctg cgcgacattt ccgcgccgct
     1081 gggtttgacg atcgcggagg tcaggtggca tgaacaggac gtcgtctggg tgcgtgctat
     1141 cgatgggttc tacaaccgca tctttcgcat tcgccgcggc gagagcccgc agcgtatcga
     1201 cttgccactc tcggcggccg cattcgatgt atccaaccag ggcgtgctcg cctatgtggg
     1261 acaggactat cagcatttgc ccgagatcta tctcagaggc gcggacggca gcctgcgtca
     1321 gatgacgcat ctacagcagg gctgggaagg catctcgctg gcgtcaagca cgatctttcg
     1381 gaccgtgact gccgacaatc tgtcaatcga ggcggcattg ttgcaaagcc ctagcagcgt
     1441 ggacggtcac aaacgaccgt tagtgctgct ggtgcacggt ggaccgccct cgaatttcag
     1501 cgccggatat gactgggaag aagcctgggc tcagctgctg gtgtcccgcg gctatcaggt
     1561 tctgatggtc aacccccgcg gttccgatgg ctacagcgag gcctttctca aagccaatcg
     1621 cgctgactgg ggcgggggcg actaccggga tctcatgagc gttctcgacg cagtcgtggc
     1681 caggggtagc acggatgcgg cgcggatcgg aatcggtggt tggtcttacg gtggcgagat
     1741 ggccgcctgg gcgatcaccc agaccgaccg gttcaaggct gcggttgcgg gtgcatgcgt
     1801 tttcgatcag gccggcgagt tcgagaccga agaggacgat ccggccgcgg acgaatggta
     1861 cttcggcacg ccctgggagc atcctgacgt gtttgcgcgc aattcgcccg taacgttcat
     1921 cggacaggcg cacacgccga cgctgatact cgatggcgaa gacgacgcga acaaccctgt
     1981 cggtcagtcc aaaggactct atcgcgcgct caagcacctc gg
//
LOCUS       DATMRM010000101         2733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2212208, whole genome shotgun sequence.
ACCESSION   DATMRM010000101 DATMRM010000000
VERSION     DATMRM010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2212208"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..344)
                     /locus_tag="VNZ06_03050"
     CDS             complement(69..344)
                     /locus_tag="VNZ06_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19758.1"
                     /translation="MGANPDSDEYEEDDAEEVAGDDDLDLDRAIKDMEIAKRRAVKVG
                     EPAWRRLEQRLEQKRTAELISDFEDYDINGEGDDLDEPHSGHSKPHM"
     gene            complement(432..1652)
                     /gene="gspF"
                     /locus_tag="VNZ06_03055"
     CDS             complement(432..1652)
                     /gene="gspF"
                     /locus_tag="VNZ06_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HWW19759.1"
                     /translation="MGAFEYTALDSAGRSRKGVIEGDTPRHVRSLLRERQLLPLTVEQ
                     VAEQESKRQRGSRFGFARGVSAADLSLMTRQLATLAKAGLPLEEALLAVSQQTEKPRV
                     QSIVLGVRAKVVEGHTLATGLADFPRVFPEIYRATVSAGEQSGHLDAVLERLAEYTES
                     REAIRQKVVGALLYPIVLTVMCFGIVSILLTYVVPKVTDVFEAQHAQLPVATRILVGI
                     SDFMRNDGLYLLIGLALAGFVFWRAMKSPAKLRRFHIFILTIPVLGRIMRGFNTARFT
                     RTLSILVESAVPVLDSLRISSEVVTNLPMRDAVTDAAARVREGAPIGRSLAASRLFPP
                     MTIHLISSGESSGELQAMLERAATNQERELEGLLAALVGLLGPLLIVGMGLVVMGIVF
                     AMLLPIFELNQLIH"
     gene            complement(1670..>2733)
                     /locus_tag="VNZ06_03060"
     CDS             complement(1670..>2733)
                     /locus_tag="VNZ06_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWW19760.1"
                     /translation="NRLVIRFRVDGVLREVLQTKRAVAPLVVSRIKVMSKLDIAEKRL
                     PQDGRISLRIAGRAVDVRVSTIPSGHGERVVLRLLDKQAGRLDLDSLGMAPDTQKLID
                     ELIHKPHGILLVTGPTGSGKTTTLYAALEQLNDNTQNIMTVEDPIEYYIDGIGQTQVN
                     TKVEMTFARGLRAILRQDPDVVLVGEIRDLETAQIAVQASLTGHLVMSTLHTNTAAGA
                     LTRLRDMGIEPFLLSSSLIGVLAQRLVRVLNPDTRQAFTAGEYERRLLGLGPNDPSPT
                     LYRPGRDAAGGYRGRTGIYELILVDDTLRTMIHDGVSEHEIEKYARQFTPSIRDDGLA
                     RCLKGETTIEEVLRVTRED"
BASE COUNT          506 a          865 c          845 g          517 t
ORIGIN      
        1 gtcgtcgtcc gcgacgggta gcacgacggc gcgcaccact tgctgaccga ccttaggggc
       61 tcaagcgact acatgtgcgg cttggaatgc ccgctgtggg gctcgtccag atcgtcgcct
      121 tcaccgttga tgtcgtagtc ctcgaaatcg ctgatcaact cggcggtgcg tttctgctca
      181 agtcgctgct cgagccgccg ccatgccggt tcgccgacct tcacggcgcg gcgtttggca
      241 atttccatgt ccttgatggc gcgatccaag tcgaggtcgt cgtcgccggc gacctcttcc
      301 gcgtcatctt cctcgtattc gtccgagtcc ggatttgcgc ccatggtgct gacctaaatc
      361 tgaatcctta ggtgtagcaa actctccaga gccgaactta atgcgattca aaaaacgatg
      421 caacggtgtg gtcaatgtat tagttgattc agttcaaaaa tcggtaacaa catcgcaaat
      481 acaataccca tgaccacaag ccccatgccg acgatcagca gcggtccgag cagaccgacc
      541 agcgcggcca gtaggccttc aagctcgcgt tcctgattgg tcgcggcgcg ctccagcatt
      601 gcctgcagtt cgccgctgga ttcaccgctg gaaatcagat ggatcgtcat cggcgggaac
      661 agccggctgg ccgcgagcga gcggccgatc ggtgcaccct cgcgtacgcg cgcggccgcg
      721 tcagtcaccg cgtcgcgcat cggcagattg gtcaccactt cgctggagat gcgcagtgaa
      781 tcgagcactg gcacggcgct ttccaccaga atgctgagcg tgcgggtgaa acgcgcggta
      841 ttgaaaccgc gcatgatccg accaagcacc ggaattgtca ggataaagat atgaaagcgg
      901 cgtagcttgg cgggcgattt cattgcacgc cagaacacaa agcccgccag cgccagcccg
      961 atcagcagat acagtccgtc gttgcgcatg aagtcgctga tgccgaccag gatccgcgtg
     1021 gccactggca gctgcgcgtg ctgcgcctca aatacatcgg tgaccttcgg taccacgtag
     1081 gtcagcagaa tcgagacgat gccgaaacac atgacggtca gcacgatcgg gtagagcagc
     1141 gcgccgacca ccttttgccg gatcgcctcg cgcgactcgg tgtattccgc cagacgctcc
     1201 agtaccgcat ccaggtgacc ggactgctca cccgcagaga cggttgcgcg atagatctcg
     1261 gggaataccc gtgggaagtc ggccagtccg gtggcgagcg tgtgaccttc gaccactttg
     1321 gcgcgcacac cgagcacgat gctctgcacc cgcggtttct cggtctgttg cgataccgcc
     1381 agcagcgctt cttcgagtgg caggccggcc ttcgccagcg tggcgagctg gcgcgtcatc
     1441 agcgacagat cggctgccga aacgccgcgt gcgaaaccga agcggctgcc ccgttgacgt
     1501 ttggattcct gttcggcaac ctgctcgaca gtcagcggca acagttgccg ctcacgcagc
     1561 aacgagcgta cgtgacgcgg cgtgtcgcct tcgatcacgc ctttgcgtga gcgaccggcg
     1621 ctgtctaacg cggtgtattc aaaagctccc attgagagcg aagtctagtc tagtcctcgc
     1681 gggtgacgcg caggacttct tcgatcgtgg tctcgccctt caggcagcgt gccaggccgt
     1741 catcgcggat cgacggcgtg aactgacggg cgtacttctc gatttcgtgt tcgctgacgc
     1801 catcgtggat catcgtgcgc agcgtgtcgt ccaccaggat cagctcatag atgccggtgc
     1861 ggccgcggta gccgcccgcg gcgtcgcggc ctggtcgata cagcgtcggc gagggatcat
     1921 tcggacccaa gcccagcagg cggcgctcgt actcgccggc ggtgaaggcc tgacgcgtat
     1981 ccggattgag tacccgcacc agacgctgcg ccagcacacc gatcagactg gaagacagga
     2041 gaaaaggctc gatgcccatg tcgcgcaggc gcgtgagcgc gccggcagcc gtattggtgt
     2101 gcagggtgga catcaccagg tgaccggtga gactggcctg cacggcgatc tgcgcggtct
     2161 ccaggtcgcg gatttcgccg accagcacca cgtcagggtc ctggcgcagg atcgcgcgca
     2221 ggccccgagc aaaggtcatc tccaccttgg tgttgacctg cgtctggccg atgccgtcga
     2281 tgtagtactc gatcgggtcc tcgaccgtca tgatgttctg ggtgttgtcg ttgagctgct
     2341 cgagcgcggc atagagcgtc gtggtcttgc ccgaaccggt cgggccggtg accagcaaaa
     2401 tgccgtgcgg tttgtggatc agctcatcga tcagcttctg cgtgtccggc gccatgccca
     2461 gcgagtccag gtccaaccgc ccggcctgct tgtcgagcaa gcgcagcacc acgcgctcgc
     2521 cgtgaccgga agggatggtg gagacacgca cgtccacggc tcggccggca atgcgcagac
     2581 tgatgcgccc gtcctgcggc aagcgcttct cggcgatgtc gagcttggac atcaccttga
     2641 tgcgcgacac caccaatggc gccaccgcgc gcttggtttg cagcacctca cgcagcacgc
     2701 cgtccacgcg gaagcggatt accagccgat tct
//
LOCUS       DATMRM010000102         3511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_676390, whole genome shotgun sequence.
ACCESSION   DATMRM010000102 DATMRM010000000
VERSION     DATMRM010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676390"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2286
                     /gene="glgP"
                     /locus_tag="VNZ06_03065"
     CDS             <1..2286
                     /gene="glgP"
                     /locus_tag="VNZ06_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HWW19761.1"
                     /translation="EDEPLVAYFCAEYGFHESFPIYSGGLGILAGDHCKAASDERLNF
                     VAVGLLYTQGYFTQSVNSDGVQQASYNDTDPRDLPVEAVRDAAGNWLQVKVLIAAREV
                     IARIWKAQVGRVSVYLLDTNCKDNAAEDREITHRLYGGDASVRIRQEMILGLGGLRAL
                     RLLGLTPKVWHMNEGHAAFLILELVREGIAQGMSFAASLEAAAAQCVFTTHTPVAAGH
                     DAFDMGLVTHCFGDFFAQMGIEQDRVFDLGRSPQAGHVFNMTHLALNGARRVNGVSRI
                     HGRVSSELCASHWPEIPPHENPVGYVTNGVHVPTFLAQTWGRFFDATLGPGWRDRLSD
                     REFWIELANVPDFDFWETAQQVKARMLEGVRGRLRREYLAKGLLPAQLRRITRLLDPE
                     RPDVLTLGFARRFATYKRASLLLRDRARLLRLIKAEDKPVLFLFAGKAHPADQPGQQV
                     LREIKQLMQVPEIAGHVVFLEDYDLQLARWLVTGVDVWLNNPVAPLEASGTSGIKAAV
                     NGRLNVSILDGWWAEAFDHNNGWGIPGSDAQDVERRDALDAAAMLDSIEEEVVPLYYA
                     RNDEGYSPEWVRRSKRAMMTVIPDFNMGRVVQDYARGLYFPAAQYHERLTRDGIGAAT
                     QLASWKQQVRERWSGVGLRRLSDPPREMPRAGALNVRVSAGLNGLTTADVRVEFVAQR
                     VLPRSRSSMPALSSFRSDRRDDAWRALLRPTGETDADGSAVYELNAPPPNCGQFAYEI
                     RIYPWHDMLSHPLELGLLKRL"
     gene            2300..>3511
                     /gene="glgC"
                     /locus_tag="VNZ06_03070"
     CDS             2300..>3511
                     /gene="glgC"
                     /locus_tag="VNZ06_03070"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HWW19762.1"
                     /translation="MAKQQSRASSGRYVSRLTSGALAVVMAGGRGERLKDLTLHRCKP
                     ATPFGGKFRIIDFVLSNCVNSGIRQIFLMTQYKGQSLIQHVQRGWSYLRGEFGEFIDV
                     VPAQQQVGKHWYRGTADCVYQNLELIRALRPKHVLVLAGDHIYKMDYGPMIAYHVEKG
                     ADITVGVVEVPRDRAIEFGVLSVTDWNRVTKFTEKPRDPESIPSRPDVALASMGIYVF
                     NARLLEKLLLADAADDHSQHDFGKDVIPAAIDILHVVAYPFTDVKTRAQNYWRDVGTV
                     DAYYDANLELVHVAPELNIYDEEWPIWTYQAHQPPAKFVLDEDGRRGAAVNSMVSGGC
                     IVSGAFVNQSLLFSNVRVEERSSIHRAVVLPNVKVGAGCSISNAIIDENCEIPDGMKI
                     GQDRAADRKRFY"
BASE COUNT          645 a         1043 c         1142 g          681 t
ORIGIN      
        1 gaggacgagc cgctggtggc gtatttctgc gccgaatatg gctttcacga aagtttcccg
       61 atctactccg gcggcctggg cattcttgcc ggagatcact gcaaggccgc cagcgacgag
      121 cgactcaatt tcgtcgcggt gggcctgctc tacacccagg gctacttcac ccagtccgtc
      181 aacagcgatg gtgtgcagca ggccagttac aacgacaccg atccgcgcga tctaccggtg
      241 gaagcggtgc gcgatgctgc cggcaactgg ctgcaggtga aggtgctgat cgccgcacga
      301 gaggtgatcg cccgcatctg gaaagcgcag gtgggccgcg tctccgtcta tcttctcgat
      361 accaactgca aggacaacgc cgcggaagac cgcgaaatca cgcatcggct gtacggtggt
      421 gatgccagcg tgcggattcg ccaggaaatg attcttggtc tgggcggcct gcgcgcgctg
      481 cgcctgctcg ggctgacgcc caaggtctgg cacatgaacg agggccatgc cgcgttcctg
      541 atactggaat tggtgcgtga aggtatcgcg caaggcatga gctttgcggc ctcactggag
      601 gccgccgccg cccagtgcgt gttcacgacg catacaccgg tcgccgccgg tcatgatgcg
      661 tttgatatgg gactggtcac gcattgcttt ggagatttct ttgcgcagat gggcatcgaa
      721 caggatcggg tgttcgatct gggacgctcg ccccaggccg gtcacgtgtt caacatgacg
      781 catctggcgc tcaacggcgc gcgacgcgtg aacggggtga gccgcataca cgggcgagtc
      841 tcctcggagc tgtgcgcctc gcactggccg gaaataccac cccacgaaaa tcctgttggc
      901 tatgtgacca acggtgtgca cgtgccgacc tttctggcgc agacctgggg ccggtttttc
      961 gacgccacac tggggccggg ctggcgcgac cggctgtcgg accgggaatt ctggattgag
     1021 ctggcgaatg taccggattt tgatttctgg gaaaccgcgc agcaggtgaa ggctcgtatg
     1081 ctcgaaggcg tgcgcggccg gctgcgacgc gagtatctgg cgaagggtct gttacccgcg
     1141 cagctgcgcc gtatcacgcg actgctcgat ccggagcgtc cggacgtgct gacgctcggt
     1201 ttcgcacgcc gcttcgcgac ctacaagcgc gcctcgttgc tgctgcgtga ccgcgcgcgg
     1261 ctgctgcggc tgatcaaggc cgaggacaag cctgtcctgt tcctgttcgc gggcaaagcc
     1321 catcccgcgg atcagcccgg gcaacaggtg ctgcgcgaaa tcaaacagct gatgcaggtg
     1381 ccggagatcg ccggtcacgt ggtgttcctc gaagactacg atctgcaact ggcgcgctgg
     1441 cttgtgaccg gcgtggatgt ctggctcaac aatccggtcg cgccgctgga agccagcggc
     1501 acttcgggca tcaaggccgc cgtcaacggg cgtctgaacg tcagcatcct cgatggctgg
     1561 tgggcggaag ccttcgatca caataatggc tggggcattc cgggttcgga tgcgcaggac
     1621 gtggaacgtc gcgatgcgct cgatgccgcg gcgatgctcg attcgatcga agaggaagtg
     1681 gtgccgctct actacgcccg caacgacgag ggctattcgc cggagtgggt gcgtcgcagt
     1741 aagcgcgcga tgatgactgt gatcccggac ttcaacatgg gccgggtcgt ccaggactat
     1801 gcgaggggcc tgtactttcc ggccgcgcaa tatcatgaga ggctcacgcg tgacggcatc
     1861 ggtgccgcca cccagctcgc ctcatggaaa cagcaggtgc gcgagcgctg gtcgggtgtc
     1921 ggtctgcgcc gactgagcga tccgccgcgc gaaatgccgc gcgcgggtgc tttgaacgtg
     1981 cgggtaagcg ccgggctgaa tggtttgacg accgccgacg tgcgcgtgga gttcgtcgcg
     2041 cagcgagtgc tcccaaggtc acgaagttcg atgcctgcac tgtcctcgtt ccgcagtgat
     2101 cgccgcgatg atgcctggcg ggcgttgctg cgtccgaccg gggagactga cgcggacggt
     2161 tcggcggtct atgagctcaa tgcgccgccg ccgaactgcg gtcagtttgc ctatgagatc
     2221 cgcatttacc cctggcatga catgctgtct catccgctcg aactgggatt gctcaaacga
     2281 ctctagaatc aggagcacta tggcaaagca gcagtcgcgg gcttcctcgg ggcgctatgt
     2341 cagccgcctg acgagcggcg cgctggctgt cgtgatggcc gggggtcgcg gggagcggct
     2401 caaggacctg accctgcatc gatgcaagcc agcgacgccg ttcggcggta aattccgcat
     2461 catcgatttc gtactctcga actgcgtgaa ctcaggcatc cggcagattt tcctgatgac
     2521 gcagtacaaa ggtcagtcgc tcattcagca tgtgcagcgc ggctggagct atctgcgcgg
     2581 cgaattcggc gaattcattg acgtcgtgcc ggcgcagcaa caggtgggta agcactggta
     2641 tcgcggcacg gccgactgcg tttatcagaa tcttgagctg atccgtgcgc tgcgccccaa
     2701 gcacgtactg gtgctggccg gggatcacat ctacaagatg gactatggtc cgatgattgc
     2761 gtatcacgtc gagaagggcg cggacatcac cgtgggcgtg gtcgaagtcc cgcgcgatcg
     2821 cgcgatcgaa ttcggtgtgc tatcggtaac ggattggaat cgcgtcacca agttcacgga
     2881 aaaaccgcgc gatcctgaat cgattccttc gcgtccggac gtcgccctgg catcgatggg
     2941 aatctacgtc ttcaacgccc ggctgctgga aaaactgctg ctcgccgatg cggccgatga
     3001 ccattcccag cacgacttcg ggaaagacgt gataccggcc gccatcgata tcctgcatgt
     3061 ggtcgcttat ccgttcaccg acgtcaagac ccgcgcacaa aactactggc gtgacgtcgg
     3121 cacggtcgac gcgtactacg acgccaatct cgagctggtg catgtcgcgc ccgaactgaa
     3181 catctacgac gaggaatggc cgatctggac ctaccaggcg catcagccac cggcgaagtt
     3241 cgttctcgac gaggatggac gacgtggtgc agcggtcaac tcgatggtat ccggcggttg
     3301 catcgtctcc ggcgcgttcg tcaaccagtc gctgctgttt tcgaacgtgc gggtggaaga
     3361 acgctcgagt attcaccgcg cggtggtgct cccgaatgtg aaggtcggcg ccggctgcag
     3421 catctcgaac gctatcatcg acgagaactg cgaaattccg gatggcatga agatcggcca
     3481 ggatcgcgcc gccgaccgca agcgcttcta t
//
LOCUS       DATMRM010000103         3781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2537910, whole genome shotgun sequence.
ACCESSION   DATMRM010000103 DATMRM010000000
VERSION     DATMRM010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2537910"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VNZ06_03075"
     CDS             complement(<1..1087)
                     /locus_tag="VNZ06_03075"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HWW19763.1"
                     /translation="MTTATTNEANEAGPIPLVACHECDLLQRESAVPPDGTLRCCRCH
                     AELYRAHPDSTDRALAFTLTCVVLFFISNLYPIVGLAVNGDLVETTLLGAVRVLYKDG
                     MWPIAGLVLATTFLMPLMQMTAMAYLLVPLQLNRRPYHADFVFRLMHLAQPWGMTEVL
                     ILGMLVALVKLAHIASVVPGVALWSFGALMLLLAAAGSAFDPHALWRRMGSFDDAASK
                     TEAAAAASAHTALRCGMLSCHSCGLLVKSTAHVHGAACPRCGAHLHARKPDSIARTWA
                     FLIAAIVMYIPANALPVMNTSSLFGSEKDTIMSGVVYLWTSGSWPLAVVVFIASVAVP
                     MLKILALMYLVISTQRRSVMLLQQRTRI"
     gene            complement(1084..2646)
                     /locus_tag="VNZ06_03080"
     CDS             complement(1084..2646)
                     /locus_tag="VNZ06_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWW19764.1"
                     /translation="MRGFAFPLRLLPALLLTIGLAIGLGGCLLGPDYVRPNVPTPANF
                     RFEASEAVDAANTRWWEQFQDPVLNDLILAALADNKDVKIAAARVDQFLGQFVTTRSA
                     LFPQVNAGFDASRQRASQRGPTPLPAGVSPVFNEFQAPLTVGWELDFFGRVRRETESA
                     RASLLASEEGRRATILSLVASVATAYINLRDLDQQLAIARATTDSRAGSVKVFQARFS
                     GGDVSQMELAQSQSEYEASLATIPQLETQIAQQEDALSVLLGRNPGPILRGRELTELV
                     LPAVPAGLPSDLIERRPDLLQAEQNLVAANALIGAARALYFPQISLTGLFGSTSIEFS
                     KLFTGPARVWSYAGSLTVPIFTAGSISGQVSQAEAQQQQALVQYQQAIQTAFQEVDDA
                     LIALQKSREQLVVQARQVEALRTYARLARLRFEGGYTSYIEVLDAERSLFNAQLGYTQ
                     TNGLMFNSLIGLYKAMGGGWVVQAEAMTGRVMGQKAVAQVASQAGDQSGTQSTNPSTT
                     PTTTAPGAEQPK"
     gene            complement(2648..>3781)
                     /locus_tag="VNZ06_03085"
     CDS             complement(2648..>3781)
                     /locus_tag="VNZ06_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW19765.1"
                     /translation="VAKAKSRPELARVTSTFNASSQQLLVDVDRDKSETLGVPIEEVY
                     SAMQTMFGSLYVSQFNRSSRLWQVILQAEPQYRLTPQDLNQLYVRSKTNNMVPLKAVV
                     NSKYVTGPDLITRFNNFPAVKITANGAPGYSSGQVINALEEVADQVMPAGYGVGWSGE
                     AYEARQSGGTSGLVFVFGLVMVFLILAAQYEKWSLPFGVLLAVPFALFGALLAILLRG
                     LENDVYFQIGLTMLVALAAKNAILIFEFAVLNREAGESVYDAAVTAATERLRPIVMTS
                     LAFILGCVPLAIALGASANSRHSIGTGVIGGMLGATVIAVFFIPMFFYVLETMSEKSG
                     GKKTPGASGGQAGDGGSGPDPLPVKPAGSGGPAVTPSAQREGD"
BASE COUNT          729 a         1241 c         1185 g          626 t
ORIGIN      
        1 agatgcgggt tcgttgctgc aggagcatga ccgagcgccg ctgcgtggag atcacgaggt
       61 acatcagcgc caggatcttc agcatcggca ccgccacgct ggcgatgaac accaccacgg
      121 cgagcggcca tgagcctgag gtccagagat aaaccacgcc gctcatgatg gtgtccttct
      181 ccgagccgaa cagcgaactg gtattcatca cgggcagcgc attcgccggg atatacatca
      241 cgatggcggc aatcaggaag gcccaggtac gcgcgatgct gtcgggtttg cgtgcatgca
      301 aatgagcgcc gcagcgcggg catgctgcac cgtgcacatg agccgtcgac ttcaccagca
      361 ggccgcagct atggcaactc aacatgccgc agcgcagcgc ggtgtgggcg gacgcggcgg
      421 cggccgcctc cgtcttcgaa gccgcatcgt cgaagctgcc catgcgccgc cagagtgcgt
      481 gtggatcgaa ggcggagccg gccgccgcca gcagcagcat gagcgcgccg aacgaccaga
      541 gcgcgacacc aggcaccacg ctcgcaatgt gcgcgagttt cacgagagcc accagcatgc
      601 cgaggatcag cacctcggtc atgccccacg gctgcgcgag atgcatcagg cgaaacacaa
      661 agtcggcgtg atacgggcgg cggttcagtt gcaacggcac cagcagatac gccatcgcag
      721 tcatctgcat caacggcatc aggaaagtgg tcgctagcac gagacccgcg atcggccaca
      781 tgccgtcttt gtagagcacg cgaaccgcac cgagcaacgt cgtctcgacg agatcgccgt
      841 tcaccgcgag tccgacaatc ggatagagat tgctgatgaa gaacagcacg acgcaggtca
      901 gcgtgaaggc cagtgcccgg tcggtgctgt cgggatgcgc gcgatagagc tccgcgtggc
      961 agcggcagca gcgcagtgtg ccgtcgggcg gcaccgcgga ttcgcgctgc agcagatcgc
     1021 attcgtggca ggcgacgaga gggatcggac cggcctcgtt tgcctcgttt gtcgttgcgg
     1081 tggtcattta ggctgctccg cgccgggcgc cgtggttgtc ggagtcgtcg acggattcgt
     1141 tgattgagtc cccgactgat cgccagcctg actggccacc tgtgccaccg ccttctgccc
     1201 catcactcgc ccggtcatcg cctcggcctg cacaacccag ccgccgccca tcgccttgta
     1261 gagaccgatc agcgagttga acatcaggcc attggtctgc gtgtagccaa gctgcgcgtt
     1321 gaacaaactg cgctcggcat ccagcacctc gatatagctc gtatagccgc cttcgaaacg
     1381 cagcctcgcg agccgcgcat acgtacgcaa cgcctccacc tggcgcgcct gcacgaccag
     1441 ttgctcgcgc gacttctgca gcgcgatgag cgcatcgtcg acttcctgga acgcggtctg
     1501 gatagcctgc tgatactgga ccagcgcctg ttgctgttgc gcctctgcct ggctgacctg
     1561 cccgctgatg ctgcccgccg taaagatcgg caccgtcagc gagccggcgt acgaccagac
     1621 gcgcgcgggt ccggtgaaca gtttcgagaa ctcgatactc gtggagccga acaggcccgt
     1681 cagcgaaatc tgcggaaaat acagcgcacg tgcggcaccg atcagggcat tggcggcgac
     1741 caggttctgc tcggcctgca gcagatcggg acggcgctcg atcagatcgg acggcagacc
     1801 tgccggtacg gcgggcagca ccagttcggt cagttcgcgt ccgcgcagga tcgggccagg
     1861 gttgcgaccg agcaggaccg ataaggcgtc ctcctgctgc gcgatctgcg tctcgagctg
     1921 cggaatcgtg gcgagcgatg cttcatattc ggactgactc tgcgcgagct ccatctgcga
     1981 gacgtcaccg ccggaaaaac gcgcctggaa tactttcacc gatccggcgc ggctgtccgt
     2041 ggtcgctctc gcgatcgcca gttgctggtc aaggtcgcgc agattgatgt aagcggtggc
     2101 caccgaggcg accagcgaaa ggatcgttgc gcggcggccc tcctcgctcg ccagcaggct
     2161 cgcgcgagcc gattcggtct cccgccgcac ccgcccgaag aagtcgagct cccagcccac
     2221 cgtcagcggc gcctggaact cgttgaagac gggactcacg cccgccggca gcggcgtcgg
     2281 tccgcgctgc gaggcacgct gacgggaggc gtcgaaaccg gcgttgacct gcggaaacag
     2341 cgccgagcgc gtcgtcacga actggccgag aaactggtcg acgcgagcgg cggcgatctt
     2401 gacgtcctta ttgtcagcga gcgcggccag aatcagatcg ttcagaaccg ggtcctgaaa
     2461 ctgctcccac caccgcgtat tggccgcatc gacggcttca ctcgcttcga accggaagtt
     2521 cgcgggcgtc ggcacgttcg ggcggacgta gtccgggccg agcaggcaac cgcccagccc
     2581 gattgccagc ccgattgtca gcagcaaggc ggggagcaaa cgcagcggga acgcaaagcc
     2641 tcgcatgtca gtcgccctca cgttgcgcgg aaggcgtgac cgcagggccg ccggagcccg
     2701 caggctttac cggaagggga tcgggaccgg aaccgccatc acccgcctgc ccgccgctcg
     2761 cacccggcgt cttcttgccg ccgctctttt ccgacatggt ttcgagcacg tagaagaaca
     2821 tcggaatgaa gaacaccgcg atcaccgttg cgcccagcat gccgccgatt acgcccgtgc
     2881 cgatcgaatg ccggctgttg gccgacgcac cgagcgcgat ggccagcggc acgcaaccga
     2941 gaatgaacgc gagcgaggtc atcacgatcg gccgcagccg ctcggttgcc gccgtcactg
     3001 cggcgtcgta caccgactcg cctgcttcgc gattgagtac cgcgaactcg aagatcagaa
     3061 tcgcgttctt ggcggcgagc gcgaccagca tggtcaggcc gatctggaaa tacacgtcgt
     3121 tttcgaggcc acgcaacaga atcgcgagca gcgccccgaa caaggcaaac ggcaccgcca
     3181 gcagcacgcc gaacggcagg ctccacttct cgtattgcgc ggcgagaatc aggaacacca
     3241 tcacgagacc gaacacgaac acgagtcccg aggtgccgcc cgattgccgc gcttcgtacg
     3301 cctcaccgct ccagcccacg ccatagcctg cgggcatcac ctggtcagcc acttcctcga
     3361 gcgcattgat gacttgcccg gagctgtagc ctggtgcgcc attcgccgta atcttgaccg
     3421 ccggaaaatt gttgaaacgc gtgatcagat cggggccggt gacgtacttc gagttcacca
     3481 ccgccttcag cggcaccatg ttgttggttt tgctccgtac gtaaagctgg ttcagatcct
     3541 gcggtgtgag acggtactgc ggttcggcct gcaggatgac ctgccacaac cggctcgaac
     3601 ggttgaactg cgagacgtag agggagccga acatcgtctg catcgcgcta tacacttctt
     3661 cgatcggcac gccgagcgtt tccgacttgt cgcgatccac gtcgaccagc aattgttgcg
     3721 acgacgcatt gaaagtggac gtgacacgcg ccagttccgg ccggcttttc gccttcgcaa
     3781 c
//
LOCUS       DATMRM010000104        12248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_47041, whole genome shotgun sequence.
ACCESSION   DATMRM010000104 DATMRM010000000
VERSION     DATMRM010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12248
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47041"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /gene="rsmB"
                     /locus_tag="VNZ06_03090"
     CDS             complement(<1..1032)
                     /gene="rsmB"
                     /locus_tag="VNZ06_03090"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HWW19766.1"
                     /translation="MSETAAAVLAEAARALSKVAFSGSSADDVLRKDSGTAAAVRAIS
                     LGTLRWYWRLQALAEALLGQTRATPPVCSLLLVSLHQLEYSRNPPEATVCSAVDAVRA
                     LGQPRASGLVNALLRRFLREREALLARALTDPAAAAAHPRWLFDAIQEHWPDQSEAIL
                     GSDNSHPPMTLRVNLARGSREQYQARLRQQGLEARALPWSHSALVLEHPVAVMELPGF
                     AQGDASVQDAGAQLASALLDTNSGHRVLDACAAPGGKTGAILEQATDIRLTAIDIDPL
                     RAARIRDNLRRINAQASVITADLGQDLSWWDGHPFDRILLDAPCSATGVIRRHPDIKL
                     LRRPGDISGL"
     gene            complement(1029..1961)
                     /gene="fmt"
                     /locus_tag="VNZ06_03095"
     CDS             complement(1029..1961)
                     /gene="fmt"
                     /locus_tag="VNZ06_03095"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWW19767.1"
                     /translation="MLRVAFAGTPEFAVPTLQALADSHHTLVGVLTQPDRPAGRGREL
                     KRSPVKDLGLRLGLSVSQPAALRDDAQRAELASWQADVLVVAAYGLILPVAALTLPRL
                     GCVNVHASLLPRWRGAAPVQRAILAGDVATGVTIMQMAAGLDTGPILAQSAFDLTGRE
                     TSQALLERLSQTGARLLIETLDRLEAGTVTPKEQSSDGVTYAGKLAKQEGQVDWQRSA
                     VEISRQVRAFNPWPIAQTLFQGQQLRLWQAVPASRGGQAGEPGQVLGMDAEQLLVRCG
                     EGSLGVERLQLAGKRAMSAREFASGRALSGMHFG"
     gene            complement(1965..2513)
                     /gene="def"
                     /locus_tag="VNZ06_03100"
     CDS             complement(1965..2513)
                     /gene="def"
                     /locus_tag="VNZ06_03100"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009682343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWW19768.1"
                     /translation="MALLTVLEFPDPRLRTRAKPVAEFDAALKQLVADMLETMYAAPG
                     IGLAATQVNVHKQLIVMDVGGETRQPQVYINPEILARDGVEVSEEGCLSVPNVYEEIE
                     RSAQVRVRACDADGASFERDLDGLAAVCLQHEMDHLAGKLFVDYLSVLKRERIRRKLE
                     KDRKERANGSTKPAARATARVI"
     gene            2733..3959
                     /locus_tag="VNZ06_03105"
     CDS             2733..3959
                     /locus_tag="VNZ06_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWW19769.1"
                     /translation="MTARQAVIACLSLSGALALGGCSLFHHRDAGQAAASAAPAAAMP
                     AAQSDTTAAVAEQQTVTDALGDADEPGTTTVIRDAGPQLKAGAPKDYVVKRGDTLWGI
                     SNMFLKDPWLWPEIWYVNPDIHNPHRIYPGDTVHLALGRDGRTALQVVSSGGGGTRLD
                     PLLRSTPLNGPIATIPYSAISAFLSRPGVMTRDEVKAAPYVVALRDMHQVAGTGSDLY
                     IKKLSGDTGTRYSVMHIDEELKDPQSGRHLGYMAIYAGTAQLTRSGPVARVTLVNAAR
                     ETLKGDVLIAETGNQTSDFLPHQPQQPIDGRIIAVVNNVLLAGQFEVAAINRGSDDGL
                     DRGTVLAIDEAQQTVDDHCAYVDGSSTCFVGHGTNLPIENEGSMLVFKTYQKMSYALI
                     LGSTVPIHISDHVRNP"
     gene            4087..5175
                     /gene="dprA"
                     /locus_tag="VNZ06_03110"
     CDS             4087..5175
                     /gene="dprA"
                     /locus_tag="VNZ06_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HWW19770.1"
                     /translation="MDLIEAQLRLGRARGLTARSVQALLGSFGEQRPHDLLALCERSS
                     LARAALLRVDRARIDADRRWLQRQAITLLDIASPDYPPQLLLIGDPPRLLYCSGRIDA
                     LRRTQLAMVGSRRPTMPGALTAREFAGELSLAGLTITSGLALGIDAASHEGALTAGGL
                     TIAVLGSGLDDIYPAEHRALAARIASSGAVISEFPPGTAPRHCHFPRRNRIISGLSRA
                     LLVIEAALGSGSLTSARSALTQGRQVFAVPGSIRNPLAHGCHELIRGGAKLIERPAQI
                     LEELHFIAPKQMLMTFQQGARERVPSRAKLDKEYEILLDAVGFEAASLDMLVDRTGLP
                     SQSVASMLLILELEGAVGRQADGQYVCL"
     gene            5226..5696
                     /locus_tag="VNZ06_03115"
     CDS             5226..5696
                     /locus_tag="VNZ06_03115"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HWW19771.1"
                     /translation="MEGSVFDILIYFFDRYLLEELPSAPERETLARDLESAGFAEVNV
                     ERALDWLADLAAQRECPNVRAEGSFRVFAPQEEARLDPQCRGLLTTLETAGILSARQR
                     EIVIDRLLALEADELDVDQVKWVVLMVLSSQPGQELAYARMEDLVFDAAQHPSH"
     gene            5774..8446
                     /locus_tag="VNZ06_03120"
     CDS             5774..8446
                     /locus_tag="VNZ06_03120"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HWW19772.1"
                     /translation="MADNLIIVESPAKARTIKKYLGRDFEVLASYGHVRDLVPKEGAV
                     DPDTGFTMQYQLIERNERHVEAIAKALKKATALHLATDPDREGEAIAWHLYEILKGRG
                     DLKHKNVHRVVFHEITRNAIREAVASPRKLSLDLVNAQQARRALDYLVGFNLSPLLWK
                     KVRRGLSAGRVQSPALRMICEREEEIARFVPQEYWTIDAQGAHSAQTFPLRLTEFRGQ
                     KVEQFSFVNEGSAREAEQTLQGATGASGMLKVLQVDRKQRRRNPSPPFTTSTLQQEAA
                     RKLGFSAQRTMRLAQQLYEGVDIGDGNVGLITYMRTDSVTLAAEAVQEIREVIARLYG
                     KEAVSEEIRVYKTKSKNAQEAHEAIRPTSAGVIPADIESKLDADQFKLYSLIWKRAVA
                     CQMAHALFDTVAVDMLAGADGPGRHVLRANGSTLIKPGYIAVYQEGQDDTADDDSDQV
                     LPAMEAGDLVKLVSVQGQQHFTEPPPRFTEASLVKALEEHGIGRPSTYASIISTLRDR
                     EYVEIESRRFTATDIGKIVCRFLTDHFHQYVEYGFTADMEDELDAVARGEEPWTVPLT
                     KFWKPFIRQVDRIERTVTREQVAQARELGRDPVSGKPMTVRMGQYGPFVQIGTKDDEE
                     KPRFAGLRPGQKMDQITLEQALELFKLPRALGNTAEGEPILANIGRFGPYVKYGAKYV
                     SLKDDPYTVTLEHALECIRIKKEADANRIIQDFGVDNIQVLNGRYGPYISNKEKNARI
                     PKDREPKSITLEEARALLQAAPLRAMRGRGRFGRHKAAASTSAKTRAAQSATATNGAG
                     APKTASATRGKSAARRAAAPAVPAAPAVSVARRAAPRPAALRRAAPKTKPAAKAKSKR
                     PARLATGAAHKAVAKSVIKRSTKPTKQTKARGTA"
     gene            8467..9339
                     /gene="rlmJ"
                     /locus_tag="VNZ06_03125"
     CDS             8467..9339
                     /gene="rlmJ"
                     /locus_tag="VNZ06_03125"
                     /inference="COORDINATES: protein motif:HMM:NF016278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HWW19773.1"
                     /translation="MKYRHSFHAGNFADVHKHVALLALLSAMQRKDKGLLYLETHAGG
                     GGYDLTSVETHHGAEAKHGVSALLAAPEPEAVELKHYRDAIDDMRRRFATPALYPGSP
                     WLAAQRLRQQDRGLCYERVPSECRSLERTLGGFRRMRVECSDGYRQLAGALPPRERRC
                     LLLIDPPYENPAADQQAALDAVATALQRLANTVIALWYPVKDARAVTPWLARVERTVK
                     VPTLGLEFWLYPRDSRAGLNGSGLLIFNPPYQFEQRSAVWQAELLRTLDRSHHGGQAT
                     RWLIAEAGVDHASA"
     gene            9326..10150
                     /gene="aroE"
                     /locus_tag="VNZ06_03130"
     CDS             9326..10150
                     /gene="aroE"
                     /locus_tag="VNZ06_03130"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWW19774.1"
                     /translation="MPAPDRYAVIGHPIAHSRSPQIHRLFAQQCQQYMIYSAIDVEPE
                     QLTTWVAKFFANAGRGLNVTIPHKQSLLTMPSRLSERARRAGALNTLARDAHGQLVGD
                     NTDGVGLIRDLTEHLGLTITAHRILLLGAGGAARGVIAPLLEMLPTQLTIANRTFERA
                     EALAKAFAALGPVRATALADLQGSFDLIINATATSLRGEAPALPASALDRRTVCYDMG
                     YSDQDTAFVRWAREHGAARACMGLGMLIEQAAESFYLWRGVRPNTAGIRAALGVSP"
     gene            10175..11977
                     /locus_tag="VNZ06_03135"
     CDS             10175..11977
                     /locus_tag="VNZ06_03135"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HWW19775.1"
                     /translation="MKPPQPSRLIALCVGVLLTAGAAGHTAAPPATTAGRAASMSPAD
                     QSMLALADDFLDHYYFPSGPTGATLAGVHLYDDRLEDFSRHEVDRQISALHDYEKRLS
                     AVDGNTLSEPVRGDRELLLSGVRSNLLTLEVIRPWEKNPDLYSSGITGSAFSIMERAF
                     APPNERLRLLIAREKQMPAALQAAHANLRNAPKIYTQIAIEQLDGDVGYFEHDLPAAF
                     AAADDPKLRQEFGSANAQVIAALKAYKSWLQKHLLPLSHGEFKLGAKTFSSKLLYDEM
                     VDTPLDRLLQIGEQNLRQNQAEFARVAKQLEPDKSARQVLAELANNYPPPAKLLEAFR
                     ADFDGLIGFLNTHQIITVPEGSPPTLQETPPFMRATTFASMDTPGPFEKVATEAYFNV
                     TLPDSSWDPKRIAGYMAQFNYPVISNVVVHEAYPGHYVQFLWMHHVDDRVRKLIGANS
                     NAEGWAHYCEQMMLDEGYGQPGVGANSERDSLMLRLGQLQDALLRNARYMVAIKMHTG
                     SMTTEQAIDYFVKEGYQSRETGEVETKRGTSDPTYLYYTLGKLQILKLRADLQLKQGG
                     SFRLKDFHDAFMRQGFPPIKLVRRALMGDDSPTL"
     gene            12004..>12248
                     /locus_tag="VNZ06_03140"
     CDS             12004..>12248
                     /locus_tag="VNZ06_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19776.1"
                     /translation="MAAHDRPLLAALSSLALTMPVLSSAQDASVGAAPSQASQSAARL
                     SYLDGSVSVLPPGAQQWDKAFLNRPLGTGDQLWSDAES"
BASE COUNT         2364 a         3880 c         3836 g         2168 t
ORIGIN      
        1 caggcctgag atgtcccctg gccgacgcag caacttgata tccgggtgtc gtcgaatcac
       61 cccggtcgct gaacagggcg catccagcag gatgcgatcg aacgggtgtc cgtcccacca
      121 gctcaggtcc tgtcccaggt ccgcggtgat cacgctcgcc tgagcgttga ttcgacgtag
      181 gttgtcacgg atccgggccg cgcggagagg atcgatgtcg atggctgtca ggcggatatc
      241 ggttgcctgc tccaggatcg ccccggtctt gccaccgggg gctgcacagg catccaacac
      301 ccggtggccg ctgttcgtat cgagcagcgc gctggccagc tgtgctcccg catcctgtac
      361 ggatgcgtcg ccctgcgcga agcccggcag ttccatgacc gctaccgggt gctcgagtac
      421 cagcgcggag tgcgaccatg gcagcgcacg ggcttcaagc ccctgctgcc gaagccgcgc
      481 ctgatattgc tcgcgggagc cccgtgccag attcacacgc agggtcatcg gtggatgcga
      541 attgtcagac cccaggatcg cctcggactg atcgggccaa tgctcctgaa tggcatcgaa
      601 cagccatcga ggatgggcgg cggcggccgc agggtcagtg agcgcccgcg ccagcagcgc
      661 ctcgcgctcg cgcagaaacc gcctcaacag tgcgttaacc agccccgacg cacgtggctg
      721 tcccaacgcc cggaccgcat cgaccgccga gcagacggtc gcctccggcg gattgcgtga
      781 gtactcgagc tgatgcagtg acaccagcag cagtgagcac accggcggcg tagcgcgcgt
      841 ctgacccagc aacgcctcgg cgagcgcctg cagccgccag taccagcgca gcgtcccgag
      901 cgaaatggcg cgaaccgccg ccgccgtgcc cgaatccttg cgcagcacat cgtcggcgga
      961 cgatccggaa aaagccactt tgctcaatgc gcgggcggcc tcggccaata ccgcagcggc
     1021 ggtctcactc acccgaagtg catcccgctc agcgctctgc ccgaagcaaa ctcgcgtgcg
     1081 gacatcgccc gcttgccggc aagttgcagc cgctcgacac ccaaggatcc ctcgccacac
     1141 cgaaccagca gctgttctgc gtccatgccc agcacttgac cgggctctcc cgcctgcccg
     1201 cctcttgagg caggcacggc ctgccacaga cgaagctgct gtccctgaaa cagcgtctgc
     1261 gcaatcggcc aggggttgaa tgcgcggacc tgtcggctga tctcgaccgc gctacgctgc
     1321 cagtccacct gcccctcttg cttcgccagc ttgcccgcat acgtgacgcc gtccgaggat
     1381 tgctccttcg gcgtaacagt accggcctca agtctgtcca atgtctcaat cagtagcctc
     1441 gcgccggttt gagacagccg ttcaagcaag gcctgcgaag tctcccgtcc cgtcagatcg
     1501 aaggccgact gggccagaat ggggccggta tccagacctg cggccatctg catgatcgtg
     1561 acgcccgtgg cgacatcgcc ggccagaatc gcgcgctgaa caggcgctgc accgcgccat
     1621 cgaggcagca gggaggcatg aacgttgacg cagccaaggc gcggcagagt caatgccgcg
     1681 accggaagga tcaatccgta ggcggcgacg accagaacat cagcttgcca cgaagccagc
     1741 tcggcgcgct gcgcgtcatc tctgagcgcg gcgggctgcg acacgctcag cccaagtctc
     1801 aggcctaaat ccttgacggg gctgcgcttg agctcgcgtc cgcgtccggc cgggcgatca
     1861 ggctgagtca ggacgccgac cagagtgtgg tgcgagtcgg caagcgcctg caaggtgggc
     1921 actgcaaact cgggcgtgcc ggcaaaagcc actctcagca tgcgtcatat gacgcgcgcg
     1981 gtagcgcgtg cagcgggctt ggtggagccg ttggcccgct ccttgcggtc cttttcaagt
     2041 tttcgacgga tccgctcgcg cttcagaacc gacagatagt ccacgaacag cttgccggcc
     2101 aggtggtcca tctcatgttg cagacaaacc gcggccaggc catccagatc gcgctcaaag
     2161 ctcgctccgt ccgcatcgca ggccctgacg cgcacctgcg ccgaccgctc gatctcttcg
     2221 tacacattgg gtaccgacag gcacccttcc tcggagactt caactccgtc ccgcgccagg
     2281 atctcgggat tgatgtagac ctgtggctgg cgcgtttcgc cgccgacgtc catgacgatc
     2341 agctgcttgt gaacattgac ctgcgtggcg gccagtccga tccccggggc ggcatacatg
     2401 gtctccagca tatcggcgac cagctgcttc agcgccgcat cgaactccgc gaccggtttg
     2461 gcgcgggtgc gcaaccgggg gtcggggaat tccaggacgg tcagcagtgc catttgcgcg
     2521 attctccggt tcctaaactt gaggaaacct tcttaaatgt tgagaaatat gcgaatattt
     2581 caaggatcag ggatctcatc gtcggtcgcg tgatccagtg cacaaagttc gcaactaacg
     2641 gtgtgcgtat gcgccctctt aagtttataa cagctaaagc agaaggccct acccgttccc
     2701 aagaggtagc agggatgtat tcgaatcaag ttatgactgc ccgacaggcc gttatcgcct
     2761 gcctgagcct ctccggggcc ttggcgctag gtggctgcag cctattccat caccgcgatg
     2821 ctggacaggc ggccgcctct gctgcgccgg cagcggccat gccggccgcg cagagcgaca
     2881 ccacggcggc agtcgcggaa caacagaccg tcacggatgc gctgggtgac gcggatgagc
     2941 cgggtaccac gaccgtcatc cgggatgccg gcccccagct caaggccgga gcgcccaagg
     3001 attatgtcgt gaagcgcgga gataccctct ggggcatttc caacatgttt ctgaaagatc
     3061 cctggctgtg gccggagatc tggtacgtca atccggacat tcacaacccg catcgcatct
     3121 acccgggcga caccgttcac ctggcgctcg gtcgcgatgg gcgcaccgcg ttgcaggtgg
     3181 tcagcagcgg tggcggtgga acgcgcctcg acccgctcct gcgcagcacg ccgctcaacg
     3241 gtccgatcgc gacgattccc tattcagcaa tctcggcgtt tctgtcacgg cccggcgtca
     3301 tgacgcgcga tgaagtgaag gcagcgccgt atgtggtcgc actgcgcgac atgcaccagg
     3361 tggccggaac gggcagcgat ctgtacatca agaagctctc gggtgatacc ggcactcgct
     3421 acagcgtgat gcacatcgat gaggaactga aggatcctca gagtggacgg catctgggat
     3481 atatggcgat ttatgccggt acggcgcagc tcacgcgcag cggtccggtt gcccgcgtga
     3541 ccctggtcaa cgcggcacgc gagactctga agggtgacgt actgattgct gaaacgggta
     3601 accagaccag cgatttcctg ccacatcagc cgcagcagcc gatcgatggc cgcatcatcg
     3661 ccgtggtcaa caatgtgctg ctggccggac aattcgaagt cgccgctatc aaccgcggct
     3721 cggacgacgg tctggatcgt ggcacggtgc tggcgatcga tgaggctcag cagaccgttg
     3781 atgatcactg cgcctacgtc gatggcagca gcacgtgctt cgtcggtcat ggcaccaacc
     3841 tgccgattga gaacgaaggc tcgatgctgg tattcaagac ctatcagaag atgagctatg
     3901 ccctgatcct gggcagcacg gttccgatcc acatcagcga tcacgtgcgc aacccctaag
     3961 cgcgcgcttc gcgttcagct cgagtcgggc ccgcgtggtg tgcgccgcgc gggcccgact
     4021 tttttgggcg tttgccgcag atattccgct gcgtgtgaac cgcgccggca cgctaggctt
     4081 taggtgatgg atctgatcga ggcgcagctg cggctgggac gtgcgcgtgg cctgacggcc
     4141 cgatcagtgc aggcactgct tggctctttc ggcgagcagc gcccgcacga tctgttggca
     4201 ctgtgtgagc gctcatcgct cgcccgtgcc gcgttgctgc gggttgatcg agcgcgcatc
     4261 gacgcggatc ggcgctggct gcagcgccaa gccatcacac tactggacat cgcgagtccc
     4321 gactatccgc cgcagctgtt gctgatcggc gatccgccgc gattgctgta ttgctcgggc
     4381 cgcatcgacg cactgcgccg gacccaattg gccatggtcg gtagccgccg tcccacgatg
     4441 ccgggcgcac tcacggctcg agagttcgcc ggtgagctat ccctggcggg actgacgatc
     4501 accagtgggc ttgcgctagg aatcgacgcg gccagtcacg aaggggcgct gacggcgggg
     4561 ggcctgacca ttgccgtgct cgggagcggg ttggatgaca tctatccagc cgaacatcga
     4621 gccctcgcag cgcgcatcgc gtcctcgggt gcagtgatca gtgaatttcc accgggtacg
     4681 gcgccccgac actgccactt cccgcgtcgg aaccgcatca tcagtggctt gagccgcgcg
     4741 ctgctcgtga tcgaagcggc cctgggcagc ggctcattga ccagcgcgcg cagcgcactc
     4801 actcagggac gccaggtgtt cgccgtacct ggatcgattc gcaaccccct cgcgcacggt
     4861 tgccacgagc tgattcgcgg gggcgcgaaa ttgatcgagc gccccgcaca gatactcgaa
     4921 gagctccatt ttattgcccc gaaacaaatg cttatgacat tccagcaggg tgctcgggag
     4981 cgcgttccga gccgtgcgaa gttggacaag gagtacgaaa tcctgttaga tgccgtcggg
     5041 ttcgaagctg ccagcctcga tatgctggtg gatcggaccg gacttccaag ccaatccgtg
     5101 gcctccatgc tgttgattct tgagctcgag ggcgccgtcg gaagacaggc ggacggccag
     5161 tatgtgtgtt tgtagtcccc ggctgcttaa gtcgcagcgc tcgcgtgtgg tatgagttga
     5221 gtgatatgga aggcagcgtc tttgacattc tgatctactt cttcgaccgc tatctgctcg
     5281 aagaactgcc ttcggctccg gaacgcgaaa cgctggcgcg cgatctggag agcgccggct
     5341 tcgctgaagt caacgtcgaa cgtgcgctgg attggctggc ggatctggcg gctcagcgcg
     5401 agtgcccgaa tgtgcgtgct gagggctcgt ttcgcgtgtt tgcgcctcag gaagaggccc
     5461 ggctcgatcc gcaatgccgg ggattgctga ccacgcttga aactgccggc attctgtcgg
     5521 cccgtcagcg cgaaatcgtg atcgatcgat tgctggcgct ggaagccgat gaattggacg
     5581 tggatcaggt gaagtgggtg gtactgatgg tactgtcgag ccagcccgga caggaactgg
     5641 cctatgcaag aatggaggat ctggtattcg atgccgctca acatccatcg cattgagctt
     5701 aagcctcgac gcctgacagc aacccgcggc tcgtccgcgg tttttcgttt aaggatgact
     5761 ccccggtagt ggcatggctg acaatctgat catcgtcgaa tctcctgcca aagcgcgcac
     5821 gatcaagaaa tacctgggca gggatttcga ggtactggcc tcctatggtc acgtgcgcga
     5881 cctggtgccc aaggaaggcg ccgtggatcc ggataccgga ttcacgatgc agtatcagct
     5941 cattgagcgc aatgagcgcc acgtcgaagc catcgccaag gcgctgaaga aagcgaccgc
     6001 actgcatctg gcaaccgacc ccgatcgcga aggcgaagcc atcgcctggc atttgtatga
     6061 gattctcaag ggccgcggcg atctcaagca caagaacgta catcgcgtcg tgttccacga
     6121 aatcacgcgc aacgcgatcc gcgaggcggt cgcctcgccg cgcaagctgt cgctcgatct
     6181 ggtgaacgcg caacaggcgc gccgcgcgct cgactatctg gtgggattca atctctcgcc
     6241 gttgctatgg aagaaagtgc gccgcggcct gtcggcgggc cgcgtgcaaa gtcccgcgct
     6301 gcgcatgatc tgcgagcgcg aggaagagat cgcacgcttc gtgccccagg aatactggac
     6361 catcgatgct cagggtgcgc attccgcgca gaccttcccc cttcggctca cggagtttcg
     6421 cgggcagaag gtcgagcagt tcagctttgt caatgaaggc agcgcacgcg aggccgagca
     6481 gacgctacag ggcgccaccg gcgcatccgg catgctcaag gtcttacagg tagaccgtaa
     6541 gcagcgccgc cgcaatccgt cgccgccatt caccacctca acgctgcagc aggaagctgc
     6601 ccgcaaactg gggttctcgg cgcaacgcac gatgcgactg gcgcagcaac tgtacgaagg
     6661 tgtggacatc ggcgatggca acgtcggcct gatcacctat atgcgtaccg attcggtaac
     6721 gctagcggca gaggcggtgc aggaaattcg cgaggtcatc gccaggctct acggcaagga
     6781 agccgtgtcc gaggaaatcc gggtctataa gaccaagtcc aagaatgcgc aggaagcgca
     6841 cgaagccatc cgcccgactt ccgccggcgt cattccggcc gatatcgaaa gcaaactcga
     6901 tgcggatcag ttcaagctct actcgctgat ctggaaacgc gcagtagcct gtcagatggc
     6961 ccatgcgctg ttcgataccg tggccgttga catgctcgca ggcgctgacg gtccaggccg
     7021 acatgtgctg cgtgccaacg gttccacgct catcaagccg ggctacatcg cggtctatca
     7081 ggaaggccag gacgacaccg ccgacgatga cagcgatcag gtactgccgg cgatggaagc
     7141 cggcgatctg gtgaaactgg tgagcgtgca gggccaacag cacttcaccg aaccaccacc
     7201 acgtttcact gaagcatcgc tggtcaaggc gttggaggag cacggtatcg gtcggccctc
     7261 gacctacgcg tcgatcattt ccacgctgcg cgatcgtgag tacgtcgaga tcgagagccg
     7321 gcgcttcacc gcgaccgaca tcggcaagat cgtctgccgt tttctgaccg atcactttca
     7381 ccagtacgtc gaatacggtt ttaccgcgga catggaagat gagctcgatg cggtcgctcg
     7441 cggcgaagag ccgtggaccg ttccgctgac gaagttctgg aagcccttca tccgtcaggt
     7501 ggaccggatc gagaggacgg tgacccgcga gcaggtcgcg caggcacgtg agctcggtcg
     7561 cgacccggtc agcggcaaac cgatgacggt gcggatggga caatacggac ccttcgtcca
     7621 gatcggcacc aaggacgacg aggaaaagcc gcgtttcgcc ggattgaggc ctggtcagaa
     7681 gatggaccag attacactcg agcaggcgct cgagctgttc aagctgccac gcgcgctggg
     7741 caacacggcc gaaggggagc cgatcctcgc caacatcggt cgcttcggcc cctacgtcaa
     7801 gtacggcgcc aaatatgtgt cgctcaagga cgatccgtac accgtgacgc tggagcacgc
     7861 actcgagtgc atacggatca agaaggaggc cgatgccaat cggatcattc aggatttcgg
     7921 tgtcgacaat attcaggtgc tgaacggacg ctatgggccg tacatcagca acaaagagaa
     7981 gaacgctcgc atccccaagg accgtgagcc gaagagcatc acgctcgaag aggcacgcgc
     8041 gctgctgcaa gcagctccgc taagagcgat gcgcggtcgc gggcgcttcg gccgtcacaa
     8101 ggccgcggcc agcaccagcg cgaagacgag ggccgcgcaa agcgccactg cgaccaacgg
     8161 cgctggcgcg cccaagacgg catcggcgac ccgcggcaaa tccgcagccc ggcgtgctgc
     8221 agcccctgcg gttcccgcgg ccccggccgt ttccgtggca cgacgtgcgg ccccaagacc
     8281 tgcggccctg aggagagctg ccccgaaaac caagcctgcg gctaaggcca agtcgaagcg
     8341 gccggcgcgt ctggcgactg gcgctgccca taaggcggtt gcaaagtcag tcatcaagcg
     8401 gtcaactaag cccaccaaac aaactaaagc gcggggcacc gcgtaagcga tttcggccac
     8461 gcgccgatga agtaccgcca cagttttcac gctggcaact ttgcggacgt gcacaaacac
     8521 gtcgcgctac tggcgttgct gagcgcgatg cagcgcaagg acaagggctt gctctacctg
     8581 gagacccacg ccggaggtgg cggctacgat ctgacgagcg tcgagacgca tcatggcgcg
     8641 gaggcaaaac acggcgtcag cgcactgctt gcggccccgg agcccgaggc agtcgagctg
     8701 aagcactatc gcgacgcgat cgacgacatg cgtcgtcggt tcgccacgcc tgcactctac
     8761 cccggttcac cctggctcgc ggcgcagcgc ctgcgccagc aggaccgggg cctgtgctac
     8821 gaacgggtgc ccagtgaatg ccgctcgctc gaacgcaccc tgggcggttt ccggcgcatg
     8881 cgcgtcgaat gcagcgacgg ctaccggcaa ctcgctggcg cattgccgcc acgcgagcga
     8941 cgctgcctgc tgctgatcga tccgccttac gagaatcccg cggccgacca acaggccgcg
     9001 ctcgatgcgg tggccaccgc gctgcaacgg ctcgccaaca ccgtgatcgc cctgtggtat
     9061 ccggtcaagg atgcgcgcgc ggtaacgccg tggctggcac gtgttgagcg caccgtcaag
     9121 gtgcccacat tgggcctgga gttctggctc tacccgcgcg attcgcgcgc cggtctgaat
     9181 ggttcgggcc tgctgatctt caatcctccc tatcagttcg agcagcgttc ggcggtgtgg
     9241 caggcggagc tcctgcgcac tctggataga tctcaccacg gcggccaggc gacccgctgg
     9301 ttgatcgccg aagcgggcgt cgatcatgcc agcgcctgat cgttacgcgg tcatcgggca
     9361 tccgatcgcg cacagccgat cgccccagat tcatcgtctg ttcgcgcagc agtgccagca
     9421 atacatgatc tattcggcga tcgatgtcga gccggaacag ctgaccacct gggtcgccaa
     9481 gttctttgcc aacgctggcc gcggtctgaa cgtcacgata ccgcacaaac agtcgctgct
     9541 gacgatgccc agtcgcttga gcgagcgggc ccgccgggca ggtgcgctga atacgctggc
     9601 gcgcgacgct cacggccagc tcgtgggcga caacaccgac ggcgtgggct tgattcgcga
     9661 tttgaccgag catctggggc tgaccatcac ggcgcatcgc attctgctgc tgggcgccgg
     9721 cggcgccgcg cgtggcgtga tcgcgccgct gctggaaatg ctgccgacgc aactcacgat
     9781 cgccaatcgc acgttcgagc gtgccgaggc gcttgccaag gcgttcgcag cgctcggtcc
     9841 ggtgcgcgcc acggcgctgg cggacctgca aggcagcttc gacctgatca tcaatgccac
     9901 cgccacgagc ctgcggggtg aagcgcccgc gctaccagcg agcgcactcg accggcgcac
     9961 cgtctgttat gacatggggt actccgacca ggacaccgcg ttcgtgcgct gggctcgcga
    10021 gcacggcgct gcgcgcgcgt gcatgggact cgggatgctg attgagcagg cagcagagtc
    10081 attctattta tggcgtgggg tgcgcccgaa cacggccggc atccgtgccg cgctcggcgt
    10141 gagcccataa cgaattcagg cgagcgaggt cacgatgaag ccaccgcagc cgagccgttt
    10201 gatcgcactg tgtgtcggcg tattgctgac ggccggagcc gccggacaca cagcggcccc
    10261 gcctgcgacc accgccggac gggcagcttc gatgagcccg gcggatcagt cgatgctcgc
    10321 gctggccgat gacttcctcg atcactacta tttccccagc ggtcccaccg gtgcgacgct
    10381 ggcgggcgtg catttgtacg atgatcgtct cgaggatttc agccgtcacg aagtcgatcg
    10441 gcagatcagt gcgctacatg actatgagaa gcgcctgtcg gcggtcgatg gcaatacgct
    10501 gtccgagcca gtccgaggcg accgcgagtt gctgctgtcg ggcgtccgca gcaacctgct
    10561 gaccctggag gtcatccggc catgggaaaa gaatcctgac ctctactcct cgggaatcac
    10621 gggcagcgct ttctcgatca tggagcgggc gttcgcgccg ccgaacgagc ggctgcgatt
    10681 gttgatcgcg cgcgagaaac agatgcctgc agcactgcag gcggcgcatg ccaatctgcg
    10741 taatgcgccg aagatctaca cacagatcgc gatcgagcag ttggacggtg acgtcggtta
    10801 cttcgagcac gatctgcccg cagcatttgc tgcagccgat gatccgaagc tgcgccagga
    10861 attcggcagc gcgaatgcgc aggtgatcgc ggcgctgaag gcctacaaat cgtggctgca
    10921 aaagcacctg ctgccgctgt cacacggcga attcaagctc ggcgccaaga ctttcagttc
    10981 caaactgctg tacgacgaaa tggtcgacac accgctcgat cgtctgctgc agatcggtga
    11041 acagaatctg cgtcagaacc aggcggagtt cgcacgcgtt gccaaacagc tcgaaccgga
    11101 caagagcgct cggcaggtat tggcggagct ggcgaacaac taccccccgc ccgccaaact
    11161 gcttgaggcc ttccgcgccg actttgacgg tctgatcggt ttcctcaaca cgcatcagat
    11221 catcacagta cctgagggtt cgccgccgac tctgcaggag acgccaccgt tcatgcgcgc
    11281 gaccaccttc gcctcgatgg acaccccggg gccattcgag aaagtcgcga ccgaagccta
    11341 tttcaatgtc acattgcctg actcttcctg ggaccccaag cgcattgcag gctacatggc
    11401 gcagttcaac tatcccgtga tcagcaacgt ggtcgtgcat gaagcgtatc cgggacacta
    11461 cgtgcaattt ctgtggatgc accatgtgga cgatcgcgtg cgcaagctga tcggagcgaa
    11521 cagcaacgcc gaaggctggg cacattactg cgaacagatg atgctcgatg agggctacgg
    11581 gcaacccgga gtgggtgcaa acagcgagcg cgattcattg atgctgcgct tgggacaact
    11641 gcaggatgcc ttgttgcgca atgcgcgcta catggtagcg atcaagatgc ataccggcag
    11701 tatgaccacg gaacaagcga tcgattactt cgtcaaagag ggatatcagt cgcgcgaaac
    11761 cggtgaagtg gaaaccaagc gaggcacgtc ggatccaacc tatctctact acacgctcgg
    11821 caagctgcag attctgaagc tgcgcgcgga cttgcagctg aaacaaggcg gtagtttccg
    11881 gctaaaggat tttcatgatg ctttcatgcg gcagggattc ccgccgatca agctggtacg
    11941 tcgcgcgttg atgggagatg attctccaac gttgtagcgt ttcgcggaca ttgaggtgca
    12001 agcttggccg cgcatgaccg cccgcttctt gctgctctca gcagcctcgc gctcacgatg
    12061 ccggtgctct cgtcagcgca ggacgcgagc gtgggcgcag cgccaagcca ggcgtcgcag
    12121 agtgcggcgc gccttagcta tctggacggc agcgtctcgg tgctgccgcc cggcgcgcag
    12181 cagtgggaca aggccttcct caatcgtccg ctgggcaccg gcgatcagtt gtggagtgac
    12241 gccgaatc
//
LOCUS       DATMRM010000105         4097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_370185, whole genome shotgun sequence.
ACCESSION   DATMRM010000105 DATMRM010000000
VERSION     DATMRM010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370185"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /gene="dnaN"
                     /locus_tag="VNZ06_03145"
     CDS             complement(<1..653)
                     /gene="dnaN"
                     /locus_tag="VNZ06_03145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HWW19777.1"
                     /translation="MKVSAAREHLLEALQSVIGVVERRQTMPILSNVLLSARDNRLRV
                     TGTDLEVELVAAAEVTVQQAGDVTVPGRKLLDIIRNLPDKANITLAREGEKFLVRGGR
                     SRFALSSLPATEFPIIEDINAQHTLQIRAHDCRRLIDKTHFAMAQQDVRYYLNGTLLE
                     TDGKSLRAVATDGHRLSWCEVPLAGKTRELHQIILPRKGVLELQRLLDGEGDIEIAIG
                     "
     gene            complement(874..2268)
                     /gene="dnaA"
                     /locus_tag="VNZ06_03150"
     CDS             complement(874..2268)
                     /gene="dnaA"
                     /locus_tag="VNZ06_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HWW19778.1"
                     /translation="MGIDFAPLFSGTRNLEASLWQRCMSALETELPEQQFNTWVRPLQ
                     AQEGHGGLRLFAPNRFVIDWIRSNLLGRLTTLMHEFGLPEAAGITLEVGSRPPVPGTT
                     TAVNGTNGAGVVARAAKNGAQSVGVPLNPEFTFDGFVEGKSNHFAKAAALQVAGDPGR
                     AYNPLFIYGGVGLGKTHLMHAIGHMITERDREARVAYVHSERFVSDMVRALQHNRINE
                     FKTAYRSLQALLIDDIQFFANKEHSQEEFFHTFNALLEGQHQVILTCDRYPREVEGLE
                     ERLKSRFGWGLTVAIEPPELETSVAILLGKAQAVGVELLEEVAFFIAKRIRSNVRELE
                     GALRRVIANARFTGSPITLDFTKEALKDLLSMQARMITVDNIQKTVADYYKVRVADLL
                     SKRRSRSVARPRQVAMALAKELTTHSLPEIGDAFGGRDHTTVMHACKRIKDLRDLEQR
                     MQEDYSNLLRTLAG"
     gene            2696..2830
                     /gene="rpmH"
                     /locus_tag="VNZ06_03155"
     CDS             2696..2830
                     /gene="rpmH"
                     /locus_tag="VNZ06_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HWW19779.1"
                     /translation="MKRTYQPSKVRRARTHGFRARMATKNGRKVLSRRRAKGRKRLTP
                     "
     gene            2868..3248
                     /gene="rnpA"
                     /locus_tag="VNZ06_03160"
     CDS             2868..3248
                     /gene="rnpA"
                     /locus_tag="VNZ06_03160"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HWW19780.1"
                     /translation="MNAASRLHATRFTLPAIRRMRRPAEFKRIYASGRRFSNEFFTAN
                     AQVNDLTWARLGMSIAARILRRAVDRNRIRRLIRESFRMHQQQLPSLDIVIGARAAAG
                     TADRVRLRAALESLWQKIANSCAV"
     gene            3215..3460
                     /gene="yidD"
                     /locus_tag="VNZ06_03165"
     CDS             3215..3460
                     /gene="yidD"
                     /locus_tag="VNZ06_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HWW19781.1"
                     /translation="MAKDSELMRRVMCLLIRAYQLCVSPWLGAHCRFHPSCSQYALQA
                     VSEHGSLRGGWLSLRRLLRCHPFHPGGYDPVPHAHDH"
     gene            3444..>4097
                     /gene="yidC"
                     /locus_tag="VNZ06_03170"
     CDS             3444..>4097
                     /gene="yidC"
                     /locus_tag="VNZ06_03170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HWW19782.1"
                     /translation="MPTTINLRVTLWLLFGMALFLNYETWLHDYPADSAPAASTTAAV
                     PAPLGSVAPTAGQIPARNDAAPVAAAAATPAAAGNALADATQSDAKAPTVHVHTDVLD
                     AEVSLAGGELKRVDLLAYPQEKNRPNVPVRLLNNDSPTSMFVLESGLAGPEGETTPTH
                     LARYQCAVQELTLARGQNELQLPLTWSDGNGVTVTQTLTFRRGDYRIDLDYKVQNASA
                     "
BASE COUNT          786 a         1289 c         1200 g          822 t
ORIGIN      
        1 ccgatcgcga tctcgatgtc gccttcgcca tcgagcaatc gttgcagctc cagcactccc
       61 tttcgcggca ggatgatctg atgcaactcc cttgtcttgc cagccagggg cacttcgcac
      121 caggacagcc gatgtccatc ggtcgctacg gcgcgcagcg atttgccatc ggtctcgagc
      181 agggtcccgt tcaggtaata acgcacgtcc tgctgggcca tcgcaaagtg agtcttgtcg
      241 atcagccttc ggcaatcatg cgcgcggatc tgcagcgtgt gctgtgcgtt gatgtcctcg
      301 atgatcggga actcggtcgc cggcagactc gaaagcgcaa agcggctacg cccaccgcgc
      361 acgaggaatt tttcgccctc acgcgccagc gtgatattcg ccttgtcggg aagattgcga
      421 atgatgtcga gcagcttgcg gccaggcacc gtgacatcgc cagcctgctg caccgtcacc
      481 tccgctgccg ctaccagttc cacctccaga tccgttccgg tcactcgcaa ccggttgtcg
      541 cgtgcgctca gcagcacgtt cgagagtatc ggcatggttt gccgtctctc cacaacaccg
      601 atcacgctct gcaaagcttc gaggagatgt tcgcgtgccg ccgagacttt catgggctgg
      661 tgttccctct aacttcttag atcttctttc ttagaaagag aatagtgatg actataaggc
      721 ctgtgagtat gtcgagaaac caccggcaac ctcatttttc tcgcattccg taagcctttc
      781 aacccggctc gcgagtgcga acaagctcac gggctgtcgg tgtgcggact gtgggtaacc
      841 ttatccacaa tctcctgcac aggaaacaca cccttatccg gccagggttc tcaacaggtt
      901 tgagtaatct tcctgcattc gttgctcgag atcgcgcaga tccttgatcc gcttgcaggc
      961 gtgcatcacg gtcgtgtgat cgcgcccgcc aaaggcatcc ccgatctccg gcaggctatg
     1021 agtcgtgagc tccttggcaa gcgccatcgc gacctggcgc ggcctggcca cggagcgact
     1081 acgccgtttc gacagcagat cagcgacgcg caccttgtag tagtcggcca ccgtcttctg
     1141 aatgttgtcc acggtgatca tgcgcgcttg catcgacaac aggtctttga gcgcttcctt
     1201 ggtgaagtcg agcgtgatcg gactgccggt gaagcgtgcg ttggcgatca cacgccgcag
     1261 cgccccttcc aactcacgga cgttggaacg aatgcgcttg gcgatgaaga acgcgacttc
     1321 ctcgagcagt tcaacgccca cagcctgtgc tttgccgagc aggatcgcaa cgctggtctc
     1381 cagttccggt ggctcgatcg ccaccgtgag tccccagccg aagcgcgact tcaggcgctc
     1441 ttcgagtccc tcgacttcgc gcggatagcg atcgcaggtc aggatgacct ggtgctgtcc
     1501 ttccagcaga gcgttgaacg tatgaaagaa ctcttcctgc gaatgctctt tgttggcgaa
     1561 gaactggatg tcatcgatca gcagcgcctg cagcgagcga taagcagtct tgaactcgtt
     1621 gatgcggttg tgctgcagag cccgcaccat atcgctgacg aagcgctccg agtgcacata
     1681 cgcgacgcgc gcctcccggt cacgttcggt aatcatatgg ccgatcgcat gcatcaagtg
     1741 cgtcttaccc agcccgacgc ccccgtagat gaacaatggg ttgtatgcgc gacccggatc
     1801 acctgccacc tgtagtgctg cggccttggc gaagtgattg ctcttgcctt caacaaagcc
     1861 atcgaacgtg aactcgggat tgagcggaac acccacggac tgagcgccat tcttcgcggc
     1921 gcgcgcgacg acacccgcac cattcgtgcc gttgacggcg gtggtcgtgc ccggcaccgg
     1981 tggacgcgag ccgacctcca gcgtgatccc agcggcctca ggaagaccga actcatgcat
     2041 cagtgtcgtc aggcgcccta gcagattcga gcggatccag tcgatcacga aacgattggg
     2101 tgcgaacaag cgcagaccgc catgaccttc ctgcgcctgc aacggtctca cccaggtgtt
     2161 gaactgctgc tccggcagtt ctgtttcgag cgcgctcatg cagcgctgcc acaacgacgc
     2221 ttccaaattg cgcgttccgg aaaaaagggg ggcgaagtct atacccacgc ctgcagcttc
     2281 gaaagcattt ctcgtccgag gaaaattccc gcttcacgcg gtcactgtta actagcggca
     2341 gtaagtgctt gccagcagag taataatcac tgccaagatt gacgcgcgca ccggacggga
     2401 gcgatctcga atcgagttat cgcgaatttg cgaccggcct gcgcgctgct gctcggcttg
     2461 gcgctgattc gctggcaggt gcctcaccag gtcagtcagc atctcaacgt ccgatcgcga
     2521 tgtcgagagt ggacggcgcc agatacctca gcgaggactg cgtgatccgc accagggcgc
     2581 gtgcggaagc ggtgttggcc tcgatcaatg aatcggacac tcgtattgac aggcggtacg
     2641 ccgcccctta ccctgccggc ccgttttttc gcaaagtttt cagggccttg aagtcatgaa
     2701 acgcacatat cagccgagca aggtgcgccg ggcacgcacg catggattcc gggcccgaat
     2761 ggccaccaag aacggccgca aggtgctcag ccggcggcgc gccaagggcc gcaagcggct
     2821 aaccccctga tatagcaggc ccggaggtgt gctgagacct cgtggcgatg aacgccgcct
     2881 ctcgactcca tgcaacccga ttcacgctcc cggccattcg ccggatgcgc cggccggccg
     2941 aattcaagcg catctatgcg agcggcaggc gattcagcaa tgagttcttt acggccaacg
     3001 cgcaggtcaa tgacttgacg tgggcgcggc tcggtatgtc catcgcggca cgaattctgc
     3061 gccgcgcagt cgaccgcaat cgtatacggc gactgattcg agagagcttt cggatgcatc
     3121 agcagcaact gccatccctg gatatcgtga tcggtgcccg cgcggctgcc ggaacggccg
     3181 atcgagtccg gctgcgtgcg gcgctcgaga gtctatggca aaagatagcg aactcatgcg
     3241 ccgtgtgatg tgtcttctga ttcgtgcgta tcagctgtgt gtcagcccat ggctcggtgc
     3301 gcattgcaga ttccatcctt cctgctcgca gtacgcactg caggctgtca gcgagcatgg
     3361 gagtttgcgc ggtggctggc tcagcctgcg tcgcctgttg cgttgtcatc cgttccatcc
     3421 tggcggctac gatccggtgc ctcatgccca cgaccattaa cctgagggtg actctgtggc
     3481 tgctgttcgg gatggcattg ttcctgaact atgagacctg gctgcacgac tacccggctg
     3541 actctgcgcc agccgcctca accaccgctg cggtgccagc accgctcggt agcgtcgcac
     3601 cgacggccgg acagattccc gcgcgcaatg acgccgcacc ggtggccgcc gccgctgcca
     3661 ctccagcagc cgcgggcaat gcgctggcgg atgcgaccca gtccgacgcc aaggcgccaa
     3721 ccgtccacgt gcacaccgac gtgctggatg ccgaggtgag cctggctggc ggtgaattga
     3781 agcgcgtcga cctgcttgcc tatccgcagg agaaaaaccg gcccaatgtg ccggtacgtc
     3841 tgctgaacaa cgattcgccg acgtcaatgt tcgtgctcga aagcgggctg gcgggccccg
     3901 agggcgagac gaccccaacg catctggcgc gctatcagtg tgccgtgcag gaactgacgc
     3961 tggcgcgggg tcagaatgag ctacagctgc cactgacctg gagtgacggc aatggcgtga
     4021 cggtcacgca gacactgacc tttcgtcgcg gcgactatcg catcgacctg gactacaaag
     4081 tccagaacgc ttcagct
//
LOCUS       DATMRM010000106         2711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_49605, whole genome shotgun sequence.
ACCESSION   DATMRM010000106 DATMRM010000000
VERSION     DATMRM010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49605"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VNZ06_03175"
     CDS             complement(<1..854)
                     /locus_tag="VNZ06_03175"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19783.1"
                     /translation="MSMAEQIVEPQAVAGMSAAERRDLALYRVMHSLPIPVASRLGAA
                     LAQRMGRRAYPNADARVAAGIGHLRPDLAVNPATLAATQVRFWAHVGRIYAEYCLLHK
                     MVPRGRVDIDDRSTFQAVLADGRPVIVPFVHLGNWETCGSVLSLNAPGRVCALSNPMP
                     PNRVQAQIAVMQHARLPGKVVTFDANVWRRALEHLEQPGGILFIAIDEVMEGRVSIPC
                     FGRPLDAESNVHKIVRIAARTGAIILPLYSERLEGARFCTRILQPLEFRKQGRLEPEE
                     QRSHVEQL"
     gene            946..1647
                     /locus_tag="VNZ06_03180"
     CDS             946..1647
                     /locus_tag="VNZ06_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWW19784.1"
                     /translation="MAVTRVNENLLGGLERPVLAFLADALPGWVVPDHLTALGVVGAV
                     LTAAGFILSRWSLSWLWLASVGLILNWLGDSLDGTVARRRGIERPRYGFFIDHTTDML
                     SQALIFLSLGLSPCAHFGVACVGLIAFLMMFVYTLIGAHVRDVMQITYLGFGATEIRA
                     LLLIGNLLILAFGILHLQSGYAPLSLLGSFSGHDVAICVISLTAAVVIILSTVREART
                     LAALDPTPGAMLGPK"
     gene            1694..>2711
                     /locus_tag="VNZ06_03185"
     CDS             1694..>2711
                     /locus_tag="VNZ06_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19785.1"
                     /translation="MEMRPALEGHAGIPQETRLLFRGLSMLDNIEVEGLLQSNQWVLA
                     RGLPPRQGGDSHRLPAHRRFNRTGRVVISLEQTFWHVRLRAALCTMAMAVRHMLGGKE
                     TLARFESLHFEDFLWRRFFARTLPASDYDIVTRNGFRVARIPWNAMHICALVTSYLGL
                     PLYPTLDTSDFDLMISETPYPATVSRRTKLVVRYHDAIPLLMPHTISDRHYHHAFHYG
                     ALRKNAANGAWFVCVSEATRNDLLSVFPQVEKRSLTIHNMVSHHYYYDQASTSARLPE
                     IIKTRSTTLPTPAAQAKLTPQLRYLLIVSTIEPRKNHLTLLSAWETLRAQRYADLKLV
                     VVGRR"
BASE COUNT          505 a          842 c          809 g          555 t
ORIGIN      
        1 tccagttgct cgacatgcga gcgttgttcc tcgggctcga gccggccttg ctttcgaaac
       61 tcgagcggct gaaggatgcg cgtacaaaaa cgggcgccct cgagccgctc actgtagagc
      121 ggaaggatga ttgcccccgt gcgcgcggcg atgcgcacga tcttgtgcac attggactcg
      181 gcgtccagag gccgtccgaa gcagggtatg gagacgcgtc cttccatgac ttcgtcgatg
      241 gcgatgaaca ggattccgcc cggttgctcg agatgctcca gtgcccgtcg ccagacatta
      301 gcgtcaaacg tcaccacctt tccgggaagg cgcgcatgtt gcatgaccgc tatctgcgcc
      361 tggacgcgat tggggggcat tggattggac agcgcgcaaa cgcggcccgg agcattgagc
      421 gacagcacgc tgccacaggt ttcccagttt cccaggtgca caaacgggac gatgacgggc
      481 cgcccatcag caagcaccgc ttgaaaggta ctgcggtcat cgatgtcgac gcgaccgcgc
      541 ggcaccatct tatgtagcag gcagtactcg gcgtaaatgc gcccaacatg cgcccagaat
      601 cgcacctgag ttgctgcaag cgtcgccgga ttgacggcca ggtccggccg cagatgaccg
      661 atgccggcgg ctacgcgtgc atcggcgttc ggataggcac gtcggcccat ccgctgcgcg
      721 agcgctgcac ccaagcgcga tgcgaccgga atcggcaacg agtgcatgac ccgatagagg
      781 gccaaatcgc gacgctcggc cgcactcatt ccggcgaccg cctggggttc tacgatctgc
      841 tctgccatac tcattcggta agcagattac agcactaact tgaggggggt agatcctttc
      901 tgtacgctgg gcctccaaac agcgagccgt tcaggtagcg caccaatggc cgtcacaaga
      961 gtgaatgaaa acttgctcgg gggacttgaa aggccggttc tggcctttct agcagatgca
     1021 cttcctggct gggtggtacc cgatcacctg actgcgttgg gcgtcgtcgg agcggtgctg
     1081 actgccgcgg gattcatcct gagtcgctgg tccctgtcat ggctctggct tgcaagcgta
     1141 gggcttatac tcaactggct gggcgattca ctcgacggca cggttgccag gcgccgcggc
     1201 atagagcgtc cgcgctatgg atttttcatt gatcacacca ccgacatgct gtcgcaagcg
     1261 ctcatcttcc tgtcgctcgg cctgtcgccc tgcgcgcact tcggtgtcgc ctgcgtcggc
     1321 ctgatcgcct tcctgatgat gttcgtctac accctgatag gcgcccacgt gcgcgacgtc
     1381 atgcagatca cctatctggg tttcggtgcg accgagattc gcgcgctgct gctcattggc
     1441 aatctgctga tactggcgtt tgggatcctg caccttcaat ccggatatgc gccgctgtcg
     1501 ctgctcggtt ccttctcggg ccatgatgtc gcgatctgcg tcatctcctt gacggcggcg
     1561 gtggtgatca ttctgtcaac ggttcgggaa gcacgtaccc tcgccgcact tgatccgact
     1621 ccgggcgcga tgctcggtcc caaatagata acaacacacg gtgaacacaa ccaaggcccc
     1681 catcagggtt ctcatggaaa tgagacccgc gctcgaaggt cacgccggta tcccgcagga
     1741 aacgcggttg ctgtttcgcg gtctgtcgat gctcgacaac atcgaggtcg agggactgtt
     1801 gcagagcaat cagtgggtac tggctcgcgg gttgccgccg cgacaaggcg gcgactcaca
     1861 ccgcctgccg gcgcatcggc gcttcaatcg cacggggcgt gtggtgattt cgctcgaaca
     1921 gacgttctgg cacgttcgcc tgcgggcagc gctgtgcacg atggcgatgg ccgtgcgcca
     1981 catgctcggt ggcaaggaga ctctggcgcg cttcgagtcg ctgcacttcg aggactttct
     2041 gtggcgcagg ttcttcgcgc gtacgctgcc agcgtcggac tacgacatcg tgacgcgcaa
     2101 cggctttcgg gtggcacgca ttccctggaa tgcaatgcat atctgcgctc tggtaaccag
     2161 ctatttgggg ctaccgctgt acccgacgct cgatacatcc gacttcgatc tgatgatcag
     2221 cgagacaccc tatcctgcca ccgtctccag gcgcaccaag ctggtggtca gatatcacga
     2281 tgcgattccg ctgctgatgc cgcacacgat ttcggatcgt cactatcacc acgctttcca
     2341 ctacggcgcg ctgcgcaaaa acgctgccaa cggcgcctgg tttgtatgcg tgtcggaagc
     2401 aacccgcaac gatctgctgt ccgtgtttcc gcaggtggag aagcgatcgc tgacaatcca
     2461 caacatggtc tcgcatcact actactacga ccaggcaagc acgtccgctc gcttaccgga
     2521 gatcatcaag actcgttcga cgacgctgcc gacgcccgcg gctcaggcga agcttacacc
     2581 gcagctgcgc tatctgctga tcgtgtcaac gatcgaaccg cgaaaaaatc atctcacgct
     2641 cctgtccgcg tgggagacgc tgcgagcaca gcgctatgcc gatctcaagt tggtggtcgt
     2701 cggccggcga g
//
LOCUS       DATMRM010000107         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4093474, whole genome shotgun sequence.
ACCESSION   DATMRM010000107 DATMRM010000000
VERSION     DATMRM010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4093474"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="VNZ06_03190"
     CDS             complement(<1..1103)
                     /locus_tag="VNZ06_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWW19786.1"
                     /translation="MRSELVKPTHLDRKAVVYIRQSTPHQVVNNQESLRLQYALRQRA
                     RELGWHEADIDVIDADLGQSAASAAQRSGFKELVGRVGLSEVGLILSIDVTRLARNCS
                     DWYPLLDICGLRGCLIADRDGVYDPGSPNGRLLLGLKGSISELELHTIRSRLTAGLLA
                     KAERGELALTLPIGLIRDPSGVVVKDPDMAIQERLGLVFETFLKFRTVAKVMRVLNDR
                     GLDIPRRDRHGELRWRRATVTAVATILKNPAYAGAFVYGRTRIRTLTREGAVPSKVAR
                     PTAEWRIVVKDRYPAYIDWPTYEKIRAIVTDNRAEYMRNKTRGAPRNGELLLHGIAWC
                     ARCGHKMLVRYKGGGEYVCNHLHVHHGLPTCQH"
     gene            1601..>2383
                     /locus_tag="VNZ06_03195"
     CDS             1601..>2383
                     /locus_tag="VNZ06_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010875332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HWW19787.1"
                     /translation="MSHQHGVVQIDLAALPDDPDVLQQMLREVVPELQAENEKLWLLI
                     QRLLRHRYGPRSEQLDLEQLQLVLEDEQQSAAESEAAKDAVEPSERRRRIEVANRNRG
                     ALPAHLPRYEVVIDIENRECPCCGGVLHLIGEDRSEMLDLVPAQLRVKVVCRPRYGCR
                     TCEGAVVQAPAPERPIDGGMATEALIAHVVVSKFCDSLPLYRQAQMLKRQGITLDRST
                     LSAWVGRACWWLAPLYQLVLGTVLSSDKVFADETTLPVLEPGR"
BASE COUNT          453 a          721 c          761 g          448 t
ORIGIN      
        1 atatgctggc aggtcggcaa cccatggtgg acatgaagat gattgcacac atattcccca
       61 ccacccttgt agcggacaag catcttgtga ccgcatcgcg cacaccaagc gatgccgtgc
      121 agcaaaagct caccgttgcg aggcgctcct cgtgtcttgt ttcgcatgta ttctgcccgg
      181 ttgtccgtta cgatggcgcg gattttttca taggtcggcc agtcgatata ggcaggatag
      241 cgatccttca cgacgatccg ccattccgcc gtcggccgcg cgacctttga cggtactgcg
      301 ccttcgcgcg tgagtgtgcg tatgcgggtc cggccataca caaaggcgcc agcgtaggcc
      361 ggattcttca ggatcgtcgc aacggccgtg actgtcgctc gtctccaacg cagttcgcca
      421 tgccggtcgc ggcgcggaat gtcgagaccg cgatcgttca agacgcgcat gaccttggct
      481 acagtgcgga acttcagaaa cgtctcgaag acaagcccca gccgttcctg aatcgccatg
      541 tcgggatcct tgacgaccac accgctcgga tcgcggatga ggccgatcgg cagagtgagc
      601 gccagctcgc cgcgctcggc cttggcaagg agaccggcgg tcagccgact acggatcgtg
      661 tgcagttcga gctccgatat cgatcccttc aggccgagga ggaggcgccc attggggctg
      721 cccggatcat agacgccgtc tcgatcggcg atgaggcagc cgcgcaggcc gcaaatgtcc
      781 aagagcggat accagtcgga gcagttacgc gccagacgcg tcacatcgat tgacaggatc
      841 agccccacct cgctgagtcc gacccgacca acgagctcct taaagccgct ccgctgcgca
      901 gcggacgcgg cgctctggcc gagatccgca tcgattacat cgatgtcggc ctcatgccat
      961 ccgagttccc gagcacgctg gcgaagcgcg tattggagcc gcaggctttc ctggttgttc
     1021 accacctggt ggggcgtcga ctgccgaatg tagacaaccg ccttgcggtc caagtgtgtc
     1081 ggcttgacca gttcggacct cacgagagac ctccgccagg accgcggcga cgtcgtcgac
     1141 gatcttgcgc cgcagagccg gcggcagtac cgcccaaaga tctctcggat ctacgctcat
     1201 ggcattcgag acattcgacc gctgatataa gatcgcgaag tgcctctata cacaatttga
     1261 tctgattcgc attcacctcg taagcctcag ttacagatat tgggaccagt agtcttgtcc
     1321 cgccttgata ttctacttca tatgaaggtg gaaagttgcc acctcgatgg gtcgagcggc
     1381 ggatcacgcg gaaggatcgc ccatagagag gatgtcgcgg atccagaacc tggattacct
     1441 cgcctaatga ttcgtcagac tcaccagagg ggatattcct gtaggccctc gctggaaggg
     1501 tgcgagtggc gggcgacggc gcgcaacctg cgacccgagc tggcaggctg atgcgaatcc
     1561 tgggtgatca cgcgggattc attggctgcg acgcggcggc gtgagtcatc agcatggcgt
     1621 tgtgcagatc gatctcgcgg ccctgcctga cgatccggac gtcctgcagc aaatgctgcg
     1681 agaggtggtg cctgagttgc aggccgagaa cgagaagctg tggctgctga tccaacgtct
     1741 gctgcgccat cggtatgggc cacgctcgga gcagctcgat ctcgagcaac tgcagcttgt
     1801 cctggaggac gaacagcaga gtgccgccga aagcgaggcc gccaaggatg cggtggaacc
     1861 atcggagcga cggcggcgca tcgaggtggc caatcgcaat cgcggtgcgc tgccggcgca
     1921 cctgccgcgc tacgaggttg tcatcgatat cgagaacagg gaatgcccgt gctgcggcgg
     1981 cgtgctacac ctgatcggcg aagatcgcag cgagatgctc gatctggtgc ccgcccagct
     2041 gcgcgtgaag gtggtgtgcc ggccgcgcta tggatgccgc acctgcgagg gcgcggtggt
     2101 gcaggcaccc gcgccggagc ggccgatcga cggcggcatg gcgacggagg cgttgatcgc
     2161 gcacgtggtg gtcagcaaat tctgcgattc gctgccactc tatcgccagg cgcagatgct
     2221 caagcgccag ggtatcacac tggaccgttc cacgttgagc gcctgggtgg gacgcgcgtg
     2281 ctggtggctg gcgccgctct atcagctggt gctcggcacg gtgctgtcgt cggacaaggt
     2341 gttcgccgat gagacgacat tgccggtgct cgaacccggc cga
//
LOCUS       DATMRM010000108         3229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1298315, whole genome shotgun sequence.
ACCESSION   DATMRM010000108 DATMRM010000000
VERSION     DATMRM010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298315"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VNZ06_03200"
     CDS             <1..426
                     /locus_tag="VNZ06_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWW19788.1"
                     /translation="PRLWWWAETMGLRKFTEMLFTGRPFTAKEMHECGFVNSVVPRER
                     LEEETHKYATACSRSRPTDTVTVQKTFLEIYKQYRGEQMGSTLTAMVESLWPYMRHEP
                     DDLQFTADLMDAGLNNAVKQIEMGYPPEWRMSYSGRKKK"
     gene            complement(439..1740)
                     /gene="rhaI"
                     /locus_tag="VNZ06_03205"
     CDS             complement(439..1740)
                     /gene="rhaI"
                     /locus_tag="VNZ06_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose catabolism isomerase"
                     /protein_id="HWW19789.1"
                     /translation="MNDTSQINLSEPGVIAEFNATLLESQRAAYATLGAQLAHRQIDV
                     ERITRAVMGFQVALPSWGVGTGGTRFARFPIPGEPRDVFDKLEDCAVVHALTAATPSV
                     SLHLPWDRAEPNELKARARALGLTFDAVNSNTFQDRKGSLSYKFGSLTHTDAAVREQA
                     IEHNLECIRIGEILGSKALTLWIGDGANFPGQQHLARAFERYLESAAKIYSALPADWR
                     LLLEHKMYEPAFYATVIQDWGSSLMAAQTLGPKARCLVDLGHHAPHVNIEMIVARLIH
                     AGRLGGFHFNDSKYGDDDLDAGSIEPFRLFLIFNELVDAQWRAVPNFSPAHMLDQSHN
                     VTDPIESLISSAIELQRAYAQALLVDREQLAAYQHSNDVMMANQTLKRAFTTDVQPIL
                     AQARLNKAAAIAPLAAYRLSGYRAQCARRRPAAAGSSSGIV"
     gene            complement(1765..>3229)
                     /locus_tag="VNZ06_03210"
     CDS             complement(1765..>3229)
                     /locus_tag="VNZ06_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HWW19790.1"
                     /translation="CYANTLDVINRAIGWIGEHNTRDAGLGRLKYAPLPQASRRRIAA
                     SIMPHIRGLISKGVPKVGHFDDTPAVLAFVGSEPFERLAALGTSCPDHFLRTKIRPLV
                     LEFNPAQEDERALIERLPAAIEAYRRDYAAYYQRCKHPDSPPMRDPNAVVYLVAGVGM
                     ITFAADKATARIAAEFYINAINVMRESSNISGYRALPEQEAFDIEYWLLEEAKLKRLP
                     KPKALAGRVALITGGAGGIGLATARRLLDEGACVVLTDIDQQALDEAMSQLHSQYGRD
                     SGIGLPGDVTDETAIAAIFADTAWNYGGLDICVSNAGIASAATIEETTLKLWQRNMDI
                     LSTGYFLVSRQCVQLLKAQGLGGAIVFIGSKNALVASPGAAAYCTAKASELHLARCLA
                     LELAPAGIRVNVVNPDAVLRGSRIWQGEWAEQRARQNHTTTDELEELYRGRSLLKRSV
                     YPEDIAEAVAFLASERASKSTGNIINVDAGNAAAFAR"
BASE COUNT          622 a          966 c         1025 g          616 t
ORIGIN      
        1 ccacgtttgt ggtggtgggc ggagaccatg ggcctgcgca agttcacgga gatgctgttc
       61 actgggcgcc catttaccgc caaggagatg catgaatgcg ggttcgtgaa cagtgtggtg
      121 ccgcgcgaga ggctggagga ggaaacgcac aagtatgcaa ccgcctgctc gcgaagccgt
      181 ccgactgaca ccgtgacggt gcagaagacc tttctcgaga tctacaagca gtatcgcggc
      241 gagcagatgg gcagcacgct gactgcgatg gtcgagtcac tgtggcctta tatgcgccat
      301 gaacccgacg atctccagtt caccgctgac ctgatggatg ccgggctcaa caacgccgtc
      361 aagcaaatcg agatgggcta tccgccggaa tggcgcatga gctattcggg gcggaagaag
      421 aaataagtac gtgctgcgtt acacgatgcc gctgctcgaa ccggcggcgg ccggtcggcg
      481 gcgtgcacat tgggcgcgat agccgctcaa gcgataggcc gccagtggcg caatggccgc
      541 ggccttgttc aagcgcgcct gcgccaggat cggctgcacg tcggtcgtga aggcacgttt
      601 caaggtttgg ttggccatca tcacatcgtt gctgtgttga tacgccgcga gctgctcgcg
      661 gtcgaccagc agcgcctggg cataggcgcg ctgcagctcg atcgcactgc tgatgagact
      721 ctcaatcgga tccgtcacgt tgtgcgattg atcgagcatg tgggccgggc taaagttcgg
      781 gacggcccgc cactgcgcat ccaccagttc gttgaagatc agaaacagac gaaacggttc
      841 gatcgagccg gcgtccagat catcatcgcc gtacttggaa tcattgaaat gaaaaccgcc
      901 gagccgtccg gcatgaatca gtcgcgcgac gatcatctcg atattcacgt gcggggcatg
      961 atggcccaag tccaccagac aacgtgcctt gggtcccagc gtctgcgcgg ccatgagcga
     1021 gctgccccag tcctggatca cggtcgcgta gaaagcgggc tcgtacatct tgtgctcgag
     1081 cagcaatcgc cagtccgcag gcagcgcgga atagattttt gcggcggatt ccagataacg
     1141 ttcaaacgcg cgcgcgagat gctgctggcc cggaaagttc gcgccatcgc cgatccacag
     1201 cgtgagtgcc ttggaaccca gaatctcccc gatgcgaatg cactccaggt tgtgctcgat
     1261 cgcctgctcg cgcaccgcgg catccgtgtg tgtgaggctg ccaaatttat aggagagtga
     1321 cccctttcgg tcttggaagg tgttggaatt caccgcatcg aaggtgagtc ccaacgcgcg
     1381 cgcgcgcgct ttgagctcat tcggctcggc acggtcccag ggcaggtgca gagacacgct
     1441 aggtgtggca gcggtgaggg cgtgaaccac cgcgcagtcc tcgagcttgt cgaacacgtc
     1501 tcgaggctcg ccgggaatag gaaagcgcgc gaagcgcgtc ccgccggtgc cgacacccca
     1561 ggagggcagg gcgacttgaa aacccattac cgcgcgcgtg atccgctcga cgtcgatttg
     1621 gcgatgtgcg agctgcgcgc cgagtgtcgc atacgcggct cgctgacttt cgagcagcgt
     1681 ggcattgaat tctgcgatga cgcccggttc gctcaagttg atctggctgg tgtcgttcat
     1741 ggcgtccccc caaaacgaat ctcgtcagcg cgcgaaggcc gcggcgttgc cggcatcgac
     1801 attgatgata ttgccggtgg acttcgatgc gcgctcggaa gccaagaacg ccacggcctc
     1861 ggcgatatcc tccggataca cactgcgctt gaggagactg cggccgcgat agagctcttc
     1921 gagctcatcg gtcgtggtgt ggttctgccg cgcccgctgc tcggcccact caccttgcca
     1981 gatgcgcgag ccgcgcagca ccgcatcggg attgacgaca ttgacgcgaa taccggccgg
     2041 cgcgagctcc agggccaaac accgcgcaag atgtagctcc gaggccttgg cagtgcaata
     2101 agctgccgct cccggggagg ccaccagcgc attcttggag ccaatgaaga cgattgcgcc
     2161 gcccaggccc tgggctttga gcagttgtac acattggcga ctgacgagga aatagccggt
     2221 ggagagaatg tccatgtttc gctgccacag cttgagtgtt gtttcctcga tcgtggccgc
     2281 cgaggcgatg ccagcgttgg agacgcagat atcgagcccg ccgtagttcc acgcggtatc
     2341 tgcgaagatc gcggcgatcg ccgtctcatc cgtgacgtcg ccgggcaggc cgatgccgct
     2401 gtcgcgaccg tactggctgt gcagctggct catcgcctca tccagtgcct gctgatcaat
     2461 atcggtgagc accacacagg caccttcatc caggagccgg cgcgcggtgg ccaatccaat
     2521 gccgcccgca ccaccggtga tcagcgcgac gcgtcccgcg agcgctttcg gttttggcag
     2581 gcgtttgagc ttggcttcct cgagcagcca atattcgatg tcgaaggctt cctgttcggg
     2641 cagtgcgcga tagcctgaga tgttcgaaga ctcgcgcatg acgttgatcg cgttgatata
     2701 gaactccgcc gcgatacgcg ccgtggcttt gtcggctgcg aatgtgatca tccccacgcc
     2761 ggcgaccagg tacacgacgg cattgggatc gcgcatcggc ggtgaatcgg ggtgcttaca
     2821 gcgctggtag tacgcggcgt agtcgcgacg atacgcctcg atcgccgccg gcaagcgttc
     2881 aatcagcgcg cgctcgtctt cttgggccgg attaaactcg agcaccagcg gtcggatctt
     2941 ggtgcgcagg aaatgatcgg gacaagaggt gcccagtgct gccagccgct caaacggctc
     3001 actaccgaca aacgccagca ccgcaggggt gtcatcaaaa tggcctacct tcggtacgcc
     3061 tttggagatg agtccacgga tatgcggcat gatgctcgca gcaatccgtc gacgactggc
     3121 ctgcggcagc ggcgcatatt tgaggcggcc cagtcccgca tcacgcgtgt tgtgctcacc
     3181 gatccatccg atggcccggt tgatcacatc gagggtgttg gcgtagcac
//
LOCUS       DATMRM010000109         2913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1298512, whole genome shotgun sequence.
ACCESSION   DATMRM010000109 DATMRM010000000
VERSION     DATMRM010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298512"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VNZ06_03215"
     CDS             complement(<1..893)
                     /locus_tag="VNZ06_03215"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWW19791.1"
                     /translation="MAIDEDKLNAFMGKFVGDLGAVMHAATIVVGDSLGIYKALRNGP
                     ATVEQLAGRTQTDERYLREWLSAQVASGYVEYDPKSAAFSLTEEQAFALADEGSPAFI
                     PGAFQIAVAQFKAIPKMTERLKSGAGFGWHEHDASLFHGTERFFRPGYAANLVSQWLP
                     SLEGIEGKLKSGGRVADVGCGHGASTIIMAQAFPKSQFVGFDYHEPSVLNARSSAKKA
                     GLEGRVSFELASAKSFQGSDYDLVTMFDCLHDMGDPVGASAHVRQSLKPDGTWMIVEP
                     FANDRLEDNINPVGRIFYSAST"
     gene            complement(967..>2913)
                     /locus_tag="VNZ06_03220"
     CDS             complement(967..>2913)
                     /locus_tag="VNZ06_03220"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HWW19792.1"
                     /translation="VVLVDPTRARLILTRAFEVFQLAGDHRGCLIAATGIVETHNIEQ
                     IGIADLDRWIPVLERGIGTGVSYSTPAERVRVLTAFLVAAMLRQPSHPALPNCLREVE
                     GALELDIPLTARADMVTQLLAYFCYTNELQKARSLVARVGSMFELPELTAFRRAGWLV
                     YFSFYAALTGAHTAGIQALDALRRIVNDFGMKWFRFYDRFFRALLHLLGPSPLQASAL
                     VHELGDRVDANRFAETAQFHLARMLLFQARGDPSLAVYHGELCLTAASKTDSPYFNIL
                     FPTLIASALVEAGQLSRADELLRQARALTASTVFNHHKPLIGMVEAYRLQAQGNAEAA
                     SACLCEALRQARADHSASSLRWLVLGFRRMLALALESNIESESARGFIAELGILPESP
                     DCDSWPWPVRLRLLGGFELRSDAPPQPATGKAQKKPLEMLKVLAVQPAGELSAQALAE
                     LLWPDADGDAARESLEVTLRRLRKLIGSDDAIRWHEGRVALNESVCWVDTLAFERAHQ
                     RAERALNAPEISDVDLERTAQRLLALYPAHVLPGEEDKSWLIAGRQRLASRMFRDLTA
                     IGAHWEQRQQWEKAELTYRRAVELEAVSEVLYRRLMSVQIHRGDRAGALATYARCRQM
                     LSFTLGVEPSAETQSVRRSAMAPG"
BASE COUNT          556 a          950 c          886 g          521 t
ORIGIN      
        1 gtcgaagccg aatagaagat gcgccctacc gggttgatgt tgtcttccaa tcggtcgttg
       61 gcgaacggct cgacgatcat ccaggtcccg tccggcttga gcgactgtct cacatgggcc
      121 gaagcgccga ccgggtcgcc catgtcgtgc aggcaatcaa acatggtgac caggtcgtag
      181 tcactgccct gaaacgattt ggcgctggcc agttcgaaac tgacgcgtcc ttccagcccg
      241 gccttcttcg ctgaggatcg tgcgttcagc actgacggct cgtgatagtc gaagccgacg
      301 aactgcgact tcggaaaggc ctgcgccatg atgatcgtgg acgcgccatg accacagccc
      361 acgtcggcca cgcgcccgcc ggacttgagt ttcccctcga tcccttcgag cgagggcagc
      421 cattggctca ccaggttggc cgcgtatccg ggacgaaaga agcgctccgt tccgtgaaac
      481 agtgaggcat cgtgctcgtg ccagccgaaa ccagcaccgc tcttgagccg ttcagtcatt
      541 ttcggaatgg ctttgaattg cgcgaccgcg atctgaaacg cgcccgggat gaacgccggg
      601 ctgccctcat ccgccagcgc aaaggcctgt tcctcggtga gactgaacgc cgcggacttc
      661 gggtcgtact ccacgtatcc gctcgcgacc tgggctgaca accactcgcg cagatagcgc
      721 tcatcggtct gcgtgcgccc cgccagttgt tcgaccgtgg cagggccgtt gcgcagtgcc
      781 ttgtagatcc ccagggaatc gccaaccacg atcgtagccg cgtgcattac cgcgcccaga
      841 tcgccgacga acttccccat gaacgcgttg agcttgtctt cgtcaatggc catggtccga
      901 actccagtgt gaggtgagtg agcgaagtct gaggccgaac atccgacaaa tgaccgacag
      961 atgctcctag ccgggggcca ttgcactgcg gcggacactc tgcgtttcgg cggagggttc
     1021 gacccccagg gtgaaggaca gcatctgccg gcagcgtgca taggtcgcca gtgcacccgc
     1081 gcgatcaccg cgatggatct gtaccgacat cagccggcgg tacaacactt ccgagaccgc
     1141 ctccagttcc acggcacgcc gatacgtgag ctctgccttt tcccattgct gccgctgttc
     1201 ccagtgcgcg ccgatggcgg tcaggtcgcg aaacatccgg cttgccagtc gctgacgccc
     1261 ggcgatcagc catgacttgt cctcttctcc tggcaggaca tgagccggat acagcgctag
     1321 cagtcgctgg gccgtgcgct cgagatcgac gtccgaaatc tccggcgcat tcaacgcacg
     1381 ctcggccctc tgatgtgcgc gctcgaacgc cagggtgtcc acccagcaca cggattcatt
     1441 cagcgcgacc cgcccttcgt gccagcgtat ggcatcatcg cttccgatca gcttgcgcag
     1501 ccgccgcagc gtcacttcca gggactcgcg cgcagcgtca ccatcggcat cgggccacag
     1561 cagctccgcg agagcctgcg cgctcagttc gcccgcaggc tgcactgcca gcaccttcag
     1621 catctcgagc ggtttcttct gcgccttgcc ggtggcaggc tgtgggggcg cgtccgagcg
     1681 cagttcgaat cctcccagca gtcgcaggcg cacgggccag ggccagctat cgcagtccgg
     1741 tgattcgggc aggataccca gttcggcgat gaagccgcga gcgctctcgc tttcgatgtt
     1801 cgactccagc gcgagcgcga gcattcgccg aaaacccaac accagccagc gaagcgagga
     1861 ggcgctgtga tccgcccggg cctgtcgcag cgcctcgcaa agacaggcgc tggccgcttc
     1921 ggcattcccc tgcgcctgta gccgataggc ttcgaccatc cctatcaacg gcttatggtg
     1981 attaaagacg gtggaggcag tgagtgctcg cgcctggcgc agcaattcat ctgcgcgtga
     2041 cagctgaccg gcttccacca aggcgctggc gatgagcgtc ggaaacagaa tgttgaagta
     2101 gggagaatcc gtcttgctgg ccgctgtcag gcaaagctcg ccgtgatata cggccagcga
     2161 cggatcgccc cgagcctgaa acagcagcat gcgggccaga tgaaattggg cggtctcggc
     2221 gaatcgattg gcatcaaccc gatcgccaag ctcatgcacc aacgcgctgg cctgcagtgg
     2281 cgacggtccc agcaggtgca gcagcgcccg gaagaagcga tcgtagaagc gaaaccactt
     2341 catgccgaaa tcattgacga tccggcgcag cgcatcgagc gcctgtatgc ctgcggtgtg
     2401 cgctccggtc agtgcggcat agaagctgaa gtaaaccagc cagccggcgc gacgaaaggc
     2461 ggtgagttct ggaagctcaa acatcgatcc gacccgcgcc accagactcc gggccttttg
     2521 cagctcattg gtgtagcaga aatacgccag cagctgggtc accatgtcgg ctctggctgt
     2581 cagcggaatg tccagctcca gcgctccctc gacctcgcgc aggcagtttg gcagggccgg
     2641 gtgcgacggc tggcgcagca tcgccgcgac cagaaatgcg gtcagtacgc gcacacgctc
     2701 ggcgggggtc gagtaggaaa ccccggttcc gatgccacgt tccagcaccg gaatccaccg
     2761 atccagatct gcgatgccaa tctgctcgat gttgtgtgtc tccacgattc cggtcgcggc
     2821 aatgaggcag cccctgtgat cacccgctag ctggaagacc tcgaaagcgc gggtcaatat
     2881 caggcgggcg cgggtcggat caaccagcac gac
//
LOCUS       DATMRM010000110         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2220579, whole genome shotgun sequence.
ACCESSION   DATMRM010000110 DATMRM010000000
VERSION     DATMRM010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2220579"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VNZ06_03225"
     CDS             <1..824
                     /locus_tag="VNZ06_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWW19793.1"
                     /translation="VASSDATNIKSAIVRNGDDYIINGRKWWTSGAGDPRCKILIFMG
                     KTNPQAALHSQQSMILVPMDTPGVKMLRTLNVFGYDHAPHGHAQIDFVDVRVPAENIL
                     LGEGRGFEIAQGRLGPGRIHHCMRVIGLAERALEMLCKRATSRVTFGKRVSDHGVTLE
                     RIAEARIMIDQARWLVLHAAYMMDTVGNRAAKQQIAMIKVAAPKMACQVIDWAIQVHG
                     AGGVCDDFILAAAYAGARTLRLADGPDEVHREQIGKLELGRYRPRDVSSPSAAAK"
     gene            854..2530
                     /locus_tag="VNZ06_03230"
     CDS             854..2530
                     /locus_tag="VNZ06_03230"
                     /inference="COORDINATES: protein motif:HMM:NF006181.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWW19794.1"
                     /translation="MLNDRHFKFWPANSAWHLTAPETNLFYNAEVSAKRYPNKPYMIF
                     YGTTVTFGQFADEATRVAGLLEKSWGVRPGDRVLLMMQNCPQFAIAYYGILRANAVVV
                     PINPMAVTHDVKRYADDSGATRIIVSQELFAKVEPLLGSELKHALVATYSDYLKVPTT
                     LCVPEFISAPRQGMSHSAATRWIDMLELSVQPGPLTAGPDDLCVMPYTSGTTGVPKGC
                     MHTHRSVMHTAVAGMRWFATQPELTLMAVAPFFHVTGMQGSMSGPLYVGNTVVLLPRW
                     DRDAAAELVQRYRVSSFTAIPTMLQDFFGNPKLAQYDLSSLTRLSGGGAAMPAAVAQR
                     LEKMGVPFLEGYGLSETIAATHINPTERAKDQCLGIPIHDTDSRIIDPSTLQELPQGE
                     TGEIVTHGPQVMQGYWNKPRESAEAFIELDGKKFLRTGDLGRIDEDGYFFMIDRLKRM
                     INASGFKVWPAEVEAMMYLHPAIQEACIIAAADAHRGETVKAVIVLRPDWKGKVNEAQ
                     IIAWSREQMATYKTPRIVEFADSLPKSASGKILWRELQERENAARKSQAS"
     gene            2557..>3338
                     /locus_tag="VNZ06_03235"
     CDS             2557..>3338
                     /locus_tag="VNZ06_03235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HWW19795.1"
                     /translation="MKALISREPGGPQTLVVQDMPDPVAGPGQVRVAVRACGVNYPDL
                     LIIQDLYQIKPPRPFAPGSECAGIVDAVGAGVENVAVGDRVLLAPVRGGMAELAVGDA
                     RNCWKIPQSMPFDEAACLVMTYGTSYHALKDRAALKAGETLLVLGAAGGVGLAAVELG
                     KSMGARVIAAASSEEKLALAEEVGAESGVLYPVGPLDKADAKALTEHFKAACGNEGAH
                     VIYDAIGGDYAEAALRSIAWNGRFLVVGFPAGIPKIPLNLPLL"
BASE COUNT          631 a         1026 c         1111 g          570 t
ORIGIN      
        1 acgtcgcctc cagcgatgcc acgaatatca agtcggcgat cgtgcgcaat ggggatgact
       61 acatcatcaa cggacgcaag tggtggacct ccggtgccgg ggatccgcgc tgcaagatcc
      121 tcatcttcat gggcaagacc aatccgcagg ccgcgttgca cagccagcag tcgatgatcc
      181 tggtgccgat ggacacaccc ggagtcaaga tgctgcgcac gctgaacgtg ttcggctacg
      241 accacgcgcc gcatggccat gcacagattg atttcgtcga tgtgcgtgtg ccggcggaaa
      301 acatactgct cggcgaaggc cggggcttcg agatcgcgca gggtcggctg gggccggggc
      361 gcatccacca ctgcatgcgc gtcatcggtc tggccgagcg cgcgctggaa atgctgtgca
      421 agcgtgcgac ctcccgcgtg accttcggca agcgcgtgtc ggatcacggt gtgacgctgg
      481 agcgcatcgc cgaggcgcgc atcatgatcg atcaggcacg ctggctggtg ctgcacgcgg
      541 cctacatgat ggatacggtc ggcaaccgcg cggcgaagca gcagatcgcg atgatcaaag
      601 tggcggcccc gaagatggcc tgccaggtga tcgactgggc cattcaggtg cacggcgccg
      661 ggggtgtctg cgacgacttc attctggccg cggcctacgc cggggcgcgc accctgcgac
      721 tggcggacgg cccggatgag gtacatcgtg agcagatcgg caaactggag ctcggccgct
      781 atcgtccgcg cgatgtgagc agtccgagcg ccgccgcaaa atagatcgtc gacctctacg
      841 ggggcttcag agcatgctga acgatcgcca cttcaaattc tggccggcca actcggcgtg
      901 gcacctgacc gccccggaga ccaatctctt ctacaacgcg gaagtctcgg ccaagcgcta
      961 cccgaacaag ccgtacatga tcttctacgg cacgacggtc acattcggcc agttcgccga
     1021 tgaagcgacc cgagttgcgg gattgctcga gaagagctgg ggcgtgcgtc caggcgatcg
     1081 ggtgctgctg atgatgcaga actgcccgca gtttgcgatc gcctactacg ggatcctgcg
     1141 cgccaacgcg gtcgtcgtgc cgatcaaccc gatggcggtg acgcacgacg tcaagcgcta
     1201 tgccgatgac agtggtgcga cccggatcat tgtatcgcaa gagctgtttg cgaaggtcga
     1261 accgctgctg ggcagcgaac tcaagcatgc actggtcgcg acgtactcgg actacctcaa
     1321 ggtgccgacc acgctgtgcg tgccggaatt catctccgcg ccgcggcagg gcatgagcca
     1381 cagcgccgcc acacgctgga tcgacatgct ggagctcagt gtgcagccag gccccttgac
     1441 cgcggggccg gatgatctgt gcgtgatgcc ctacacctcc gggaccaccg gcgttcccaa
     1501 aggctgcatg catacgcatc gcagcgtgat gcataccgcc gtcgccggca tgcgctggtt
     1561 cgcgacgcaa cccgaactca cactgatggc cgtggcgccc ttctttcatg tgaccggtat
     1621 gcagggcagc atgagcgggc cgctgtatgt cggcaatacc gtggtgctgc tgccacgctg
     1681 ggatcgcgat gcggccgcgg agctcgtgca gcgctaccgg gtcagctcct tcacggcgat
     1741 tccgaccatg ctgcaggact tcttcggcaa tccgaagctc gcgcagtacg atctgtcctc
     1801 gctcacgcgt ctgagcggcg gcggcgcggc aatgccggcg gcggtcgcgc agcgactgga
     1861 aaaaatgggg gtgccgtttc tggaaggcta cggcctgtcg gagacgatcg ccgccacgca
     1921 catcaatccc actgagcgcg ccaaggatca gtgcctcgga attccaatcc acgacaccga
     1981 ttcacgcatc atcgatccga gcacgctgca ggagctgccg cagggggaga ccggcgagat
     2041 cgtgacgcac ggaccgcagg tcatgcaggg ctactggaac aagccgcggg aaagcgctga
     2101 ggccttcatc gagctggacg gcaagaagtt tctgcgaacc ggcgatctgg ggcgcatcga
     2161 tgaggacggc tatttcttca tgatcgaccg gctcaagcgc atgatcaatg cctcgggctt
     2221 caaggtatgg ccggctgaag tcgaagcgat gatgtacttg catccggcga ttcaggaagc
     2281 ctgcatcatt gccgcggccg atgcgcatcg cggcgagacg gtcaaagcgg tcatcgtgtt
     2341 acggccggac tggaagggca aggtcaacga ggcgcagatc attgcctgga gtcgcgaaca
     2401 gatggcgacc tacaagaccc cgcggatcgt ggagttcgct gactcactgc ccaaatccgc
     2461 ttccggcaaa atcctctggc gcgaactgca ggaacgcgag aacgcagcgc gcaagagtca
     2521 ggcaagctag cccactcaca ataaggcaga cccttcatga aagccctcat cagccgcgag
     2581 cccggtggcc cgcaaaccct tgtggtgcag gacatgcctg atccggtcgc aggacccggt
     2641 caggtgcgcg tcgcggtgcg cgcctgcggc gtgaactatc cggatctgct gatcattcag
     2701 gatctgtatc aaatcaagcc cccgcgccca ttcgctcccg gttcggagtg cgccggcatc
     2761 gtcgatgcgg tgggtgcggg cgtggagaac gtagcggtgg gcgatcgggt gctgctggca
     2821 ccggtgcgcg ggggtatggc agaactggcg gtgggcgatg cccgcaactg ctggaagatt
     2881 cctcagtcga tgccgtttga cgaggccgcg tgcctggtca tgacctatgg cacctcgtat
     2941 cacgcgctca aggaccgcgc cgcgctcaaa gcgggcgaaa cgctgctggt gctcggcgcg
     3001 gccggcggtg tcgggttggc cgcggtggaa ctgggcaagt cgatgggtgc acgggtgatc
     3061 gcggcggcct cgagcgagga gaagctggcg cttgccgaag aggtcggcgc cgagtcgggc
     3121 gtgctctacc cggtgggtcc gctcgacaag gcggacgcga aggcactcac cgagcacttc
     3181 aaggcagcgt gcggcaatga gggcgcgcac gtcatttacg atgcgatcgg cggggactac
     3241 gcggaggccg ccctgcgctc gatcgcatgg aacgggcgct tcctggtcgt aggatttccg
     3301 gccggtattc cgaagattcc actcaacctg ccgctgct
//
LOCUS       DATMRM010000111         4537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2221448, whole genome shotgun sequence.
ACCESSION   DATMRM010000111 DATMRM010000000
VERSION     DATMRM010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2221448"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VNZ06_03240"
     CDS             <1..751
                     /locus_tag="VNZ06_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWW19796.1"
                     /translation="RRQATEEVRRMFSMAMWMAALVAPVQIFAGDQQGLNTLKYQPAK
                     IYALEGDFQAHPDGAPLILFGIPDQRSGVVRDAIEIPKLGSLLLKHDLHAPLPGLDSF
                     PRRDWPVVGVIFWSFRIMVGLGMAILTLGLVSLFTRVRGTLHQNVWLHRAALCMGPAG
                     LIAVIAGWFTTEVGRQPFTIYHVLRTAESASPIAAPAVAASLAAFAVVYFTVFGAGVF
                     FLLRLMSKPPQSGESAMPEHGAPAHPLPAQP"
     gene            748..1755
                     /gene="cydB"
                     /locus_tag="VNZ06_03245"
     CDS             748..1755
                     /gene="cydB"
                     /locus_tag="VNZ06_03245"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HWW19797.1"
                     /translation="MIPDAALTDVWAAIVAFAVFAYVVMDGFDLGIGILFPLFDTEQE
                     RGTLMNSVAPVWDGNETWLVMGGGALMAAFPLAYAVLIPALYTPLIAMLLGLIFRGVA
                     FEFRWRTQRGRKHWDRAFFFGSALAAFMQGVALGAVLQGIHVQGRHYAGGWWDWFSPF
                     SVLTGLALLAGYASLGATWIVMKTEGPLQQHAFRLSLRAGIALITGIVAVSAATPWLQ
                     HRYYERWFAFPNILVTSQVPLLVLITVTLFLIALRRRWERLPFALTLVLFALGYVGLG
                     VSVYPYLVPDSISITDAAAPAISQRFMLVGTAILIPIILVYTAHAYWVFRGKVRTAGY
                     H"
     gene            1752..1874
                     /locus_tag="VNZ06_03250"
     CDS             1752..1874
                     /locus_tag="VNZ06_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19798.1"
                     /translation="MKTRRWSHRLAWFVGLWAASVLLTVCVAQLLRWLFARILA"
     gene            complement(2160..2642)
                     /locus_tag="VNZ06_03255"
     CDS             complement(2160..2642)
                     /locus_tag="VNZ06_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19799.1"
                     /translation="MRAVVMLGMMILELGFAGQRARATDTPKDPCSLLSPAQMRGTLG
                     DQYGAPEKSVAPRPFANTVQGTDCHYQSSRGQDQVLFRVYFDASVAQATDLHTRLKMF
                     FGKNSTPENIGDEAYLDSNDAIHVRKGNVRYFLSISHGDTPSGRKQVLALAALVAGKL
                     "
     gene            complement(2664..3596)
                     /locus_tag="VNZ06_03260"
     CDS             complement(2664..3596)
                     /locus_tag="VNZ06_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HWW19800.1"
                     /translation="MSRTVDGIMGTRIYVELWADDEKKGEAAIDAVMDEMRHIDDTMS
                     TYKPDSEVSKVNQLAAQQALPISAELFKLLQTSLDYSRITEGAFDITYASVGYMYDFR
                     ERKRPTEEQIRAALPAISYQHVILDEKAQTVHFSQPGVRIDLGGIAKGYSVDCGIDVL
                     RRLGYTHALVNAGGDSRVIGDRFGRPWIVGIRHPDHPDEIITRIPLVDAAFSTSGDYE
                     RFFDEDGVRYHHIIDPHTGRSASKVRSATVIAPTATRTDGLSKTAFILGPQEALRIYN
                     QLDDVDAVLVTPEGKVLYTKGLQPPEGTPNVRTH"
     gene            complement(3677..>4537)
                     /locus_tag="VNZ06_03265"
     CDS             complement(3677..>4537)
                     /locus_tag="VNZ06_03265"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF007989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HWW19801.1"
                     /translation="AARAIVLDAERRGVLHPGGTVVEGTAGNTGIGLAHVCNARGYRC
                     VIVMPDNQSPEKYALIEALGAEVRRVKTVPYSDPNHYQKTAGRLAQELPDAIWANQFD
                     NTANRRAHVETTGPEIWEQTGGRIDAFVSASGTGGTVAGVSEYLKSRSAKVRTVLADP
                     PGSSLYEFIKHGVVKATGSGSITEGIGIGRVTANMQDAPIDDAVHIEDPETVQLVYRL
                     LREEGLFVGSTSGVNVAAAVRVARDLGPGHTIVTVLCDTGSKYLSRLYNREWLQQKNL
                     LEAAGFTRAA"
BASE COUNT          743 a         1432 c         1392 g          970 t
ORIGIN      
        1 gcgccggcag gcgaccgaag aggtgcgccg tatgttttcg atggcgatgt ggatggccgc
       61 gttggtggca ccggtccaga tattcgccgg agatcagcag ggtctgaata cgttgaaata
      121 ccagcccgcc aagatctatg cgctcgaagg cgactttcag gcgcacccgg acggagcgcc
      181 actgatcctg tttgggattc cggatcagcg ctcgggcgtg gtgcgcgatg cgatcgagat
      241 tccgaagctt ggctcactgc tgctcaaaca cgacctgcac gcgccacttc ccggactgga
      301 cagctttcca cgccgcgact ggccggtggt gggtgtgatc ttctggagct ttcgcatcat
      361 ggtcggattg ggcatggcca tcctgacctt ggggttggtc agcctgttca cgcgcgtgcg
      421 cgggacgctt catcaaaatg tctggctgca tcgggcggcg ctgtgcatgg gaccggcggg
      481 gctcatcgcc gtgatcgccg gctggttcac gaccgaagtc gggcgccagc cgttcacgat
      541 ctatcatgtg ttgcgcaccg ctgaatccgc ctcgcccatt gccgcacccg cggtcgccgc
      601 gtcgctggcg gcgtttgccg tggtttattt caccgtgttc ggcgccggcg tgttctttct
      661 tctgcgcctg atgtcgaagc cgccacagtc cggcgagtcg gcgatgccgg agcacggcgc
      721 gccggcgcat ccgctaccgg cgcaaccatg attcccgacg ccgcactcac tgatgtgtgg
      781 gcggccatcg tcgccttcgc tgtgttcgcg tatgtcgtga tggacgggtt cgatctgggc
      841 atcggcatcc tgtttccgct gttcgacacg gagcaggagc gcggtacgct gatgaactcg
      901 gtggcaccgg tgtgggatgg caatgaaacc tggctggtga tgggtggcgg tgcgttgatg
      961 gccgcgttcc cgctcgcgta cgcggtgctg atcccggcgc tgtacacacc gctgatcgcg
     1021 atgctgctgg gcctgatctt ccgcggcgtc gccttcgagt tccgctggcg cacgcagcgc
     1081 ggccgcaagc attgggatcg cgccttcttt ttcggctccg cactcgccgc gttcatgcag
     1141 ggcgtggccc tgggcgcagt acttcagggc atccatgttc aggggcggca ctacgcgggc
     1201 ggctggtggg actggttctc accattcagc gtgctcaccg gactggcgct gctggccggg
     1261 tacgccagct tgggcgccac ctggatcgtc atgaagaccg aaggtccgct gcaacagcat
     1321 gcatttcgcc tgtccctgcg tgcaggcatc gcactgatca ccggcatcgt cgcggtcagc
     1381 gcagccacac cgtggctgca gcatcgctac tatgagcgct ggtttgcgtt tcccaacatt
     1441 ctggtgacct cgcaggtacc gctgctggta ctgatcacgg tcacgctgtt cctgatcgca
     1501 ctgcgccgac gctgggagcg tctgcccttt gcgctgacac tggtgctgtt cgcgctggga
     1561 tatgtgggcc tgggtgtgag cgtctatcca tacctggtgc ccgacagcat ctccatcacc
     1621 gatgccgcag ccccagcgat cagtcagcgc ttcatgctgg tgggcacggc gatattgatt
     1681 cccatcatcc tcgtctacac cgcgcacgcg tactgggtgt ttcgcggcaa ggtcaggaca
     1741 gcggggtatc attgaagaca cgccgctggt cccatcgtct ggcctggttt gtcggactgt
     1801 gggccgccag cgtactgctg accgtgtgcg tggcacagct gttgcgctgg ctgtttgcgc
     1861 gaatcctggc ctgaacactg cggtacgccc aggcggttac aaacgccttg ggtttgccct
     1921 atcagcgcat cacgatcgcg gcgccttgat ttactcaatg cacctggtgg agctgatggt
     1981 taacaagaat agtgtttatt aatggaccaa tttccattcc taccttggcc atgcccaatt
     2041 gcatttgttg ctgcgcgcct ggactggaca tcgccgcctg aaacttcttg tatgccggat
     2101 agccgagaag ctcgccaagc gatcgcaact cgcataaacc atcctgttgg ccatgatcac
     2161 tacagtttcc ccgcaaccag ggccgccagc gccagaacct gcttccgtcc actgggcgtg
     2221 tcgccatggc tgatcgagag aaagtagcgc acgttccctt tgcgcacatg gattgcgtca
     2281 ttgctatcga ggtaggcctc gtcaccgatg ttttcgggtg tactgttctt gccaaagaac
     2341 atctttagcc gcgtatgcag atccgtggcc tgcgccaccg aggcatcgaa gtacacgcgg
     2401 aaaagcactt gatcttgccc gcgcgagctc tggtagtgac agtctgttcc ctgcaccgtg
     2461 ttcgcgaagg gccgtggtgc cacgctcttt tccggcgcgc cgtattgatc gcccagtgtg
     2521 cctctcattt gcgctggcga cagcagcgaa caagggtcct tcggcgtatc ggtcgcccgc
     2581 gctcgttgcc ccgcaaagcc aagctccaga atcatcattc cgagcatcac caccgctcgc
     2641 atctcgcacc tcctcacgtc gaatcaatgc gttcgtacat tgggcgttcc ttccggcggc
     2701 tgcagtccct tggtatagag caccttgcct tcgggcgtca ccagcaccgc gtcaacgtcg
     2761 tcgagctgat tgtagatgcg cagcgcctct tgcggtccca gaatgaacgc agtcttggac
     2821 aggccatccg tgcgggtagc ggtcggcgcg atcacggtgg cactgcgcac tttgctggcg
     2881 gagcgcccgg tgtgcggatc aatgatgtgg tgatagcgca caccgtcctc gtcgaagaag
     2941 cgttcgtagt caccggaggt tgagaaggcc gcatcgacca gcggaatgcg tgtgatgatt
     3001 tcatccgggt gatccggatg tcggatgccg acgatccacg ggcgcccgaa gcgatcgcca
     3061 atgacccggc tgtcgccgcc ggcattgacc agggcatggg tatagccaag cctgcgcagc
     3121 acatcgatgc cgcaatccac cgagtaaccc ttggcgatcc cgcccagatc aatccgcact
     3181 cccggctgcg agaagtgtac ggtctgcgcc ttctcatcga gtatcacgtg ctggtagctg
     3241 atcgccggca gcgcggctct tatctgctcc tcggtcggcc gcttgcgttc gcgaaagtca
     3301 tacatgtagc cgacgcttgc ataggtaatg tcgaacgcgc cctccgtgat gcgtgagtag
     3361 tcgagagagg tctgcagcag cttgaacagc tcggccgaaa tcggcagcgc ttgctgcgcg
     3421 gccagctgat tgactttgga cacttcgctg tcgggcttat acgtgctcat cgtgtcgtcg
     3481 atgtgccgca tttcatccat caccgcatcg atcgcggcct cgcccttctt ctcatcatcg
     3541 gcccagagct cgacatagat ccgagtgccc atgatgccgt ctacggtgcg gctcacccat
     3601 ttcgcctggg ccgcagagct caggagtgtg gctgacagtg ccgagcaggc cagcgcggcg
     3661 agcagccgcg cacgcctcag gcggcgcggg taaatcccgc cgcttcgagc agattctttt
     3721 gctgtaacca ttcccgattg tagagacgcg acaggtactt cgagccggtg tcacacaaca
     3781 ccgtcacgat agtatgaccc gggccgagat cgcgcgcgac ccgcactgcg gccgccacgt
     3841 tcacgccact ggtgctgccg acgaacagtc cttcctcgcg cagcaggcga tagaccagct
     3901 ggaccgtctc cgggtcctcg atgtgaacgg cgtcatcgat cggcgcgtcc tgcatgttgg
     3961 cggtgacacg cccgatgcca attccctcgg tgatcgagcc gctgccggtt gccttgacga
     4021 ccccatgctt gatgaactca tacagcgaac tgcccggcgg gtcggccagc acggtgcgca
     4081 ccttggcact gcgcgatttc agatattcgg agacgccggc cacggtgccg ccggtgccgc
     4141 tggccgagac gaacgcatcg atccgcccgc cggtctgctc ccagatctcc ggtccggtgg
     4201 tttccacatg ggcgcgccga ttcgccgtat tgtcgaactg attggcccag atcgcgtcgg
     4261 gcaactcctg cgccaggcgt cccgcggttt tctgatagtg gttcgggtca ctatacggta
     4321 ccgtctttac tcggcgcact tcagcgccga gggcttcgat cagcgcatat ttctccggcg
     4381 actggttatc gggcatcacg atgacacagc gatagccgcg cgcgttgcac acatgggcaa
     4441 gtccgatacc cgtgttgccg gcagtgcctt cgactaccgt accgccgggg tgcagtacgc
     4501 cgcggcgttc ggcgtcgagc acgatcgcgc gcgcagc
//
LOCUS       DATMRM010000112         5476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1299844, whole genome shotgun sequence.
ACCESSION   DATMRM010000112 DATMRM010000000
VERSION     DATMRM010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299844"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..2241
                     /locus_tag="VNZ06_03270"
     CDS             76..2241
                     /locus_tag="VNZ06_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19802.1"
                     /translation="MNSGTIDANQFAGLNITTSGGTTNTGKLEATGGSTLTLGGTFTN
                     TGGTISATGSELQLTGATVSGGAVTLTGASLLQMTNSTIHGGSTLTNSTTGTIEVVGG
                     SNTIGGTLSNPAGGLLKVDNGAVLNLENGSYLTSGAMQLNAAGSTTELVVAGANVTLG
                     GGTLTLSNNTNNYIFGATSADTLTNQETIQGAGHIGNNQMTLVNSGTIDANQFAGMII
                     QPSGGFTNNGTLLVGSGIFMHVSGGPFSNFSGTTLTGGTYNTGGLLEIDQLGTAGGEI
                     VTNAANIVLNGANSSIVDAFGLDALSKLAVTSAGGSFSLLGGRSFTTAGNYTNNGTLS
                     VGTGSIFSVHSVFTNFSGTTLTGGTYNVTGAFQFAGANIVTNSANITLTGTTSKILNS
                     TTSADGLANFATNSSTGSFSLAGARNFTTAGNFSNAGNFLIGAGSAFTVGGPGAFTQT
                     AGTTTDNGTLSATGGVSLGGSLIGTGSVTGGLTSNGNITPGTSTTKTGILTDTGLYTQ
                     NSAGVLNISIGGTTAGSTFDVLNSSTAKLGGTLNVREVNGFVPTIGSAFKILNFTSET
                     GQFATVNGLSINGTEHFTITYEGTDVLLTVVSGAAAPANVTGNFRNTDRIARAIDTPV
                     DFADRTRSSARSGFHLEQASHPTGAPTPIGTSLHDALAGRWTSANPSHLISTKQSVSA
                     LPHSYVDGRGRLGAKVARGNLQFALPGFNSRPQLYFSVN"
     gene            2493..3266
                     /gene="modA"
                     /locus_tag="VNZ06_03275"
     CDS             2493..3266
                     /gene="modA"
                     /locus_tag="VNZ06_03275"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF007958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWW19803.1"
                     /translation="MRTAMRPLLALWLLVFGVATFAAEATRPAVIVFGAASLTNALQE
                     LGDSYTKETSVPVKFSFAASSVLARQIESGSPADIFFAADLDWMDYLQSRNLINKSSR
                     RDVLGNKLVLIAPVDSKIELKIAPHFALAAALGGGRLATGDPDSVPVGRYARSALTTL
                     GVWNDVQDHLVRAENVRGALMFVDRKEVPLGIVYETDALIDKQVRIVDVFPANSHSPI
                     TYPIALTTTAGPEAAKFVDYLLSAAGEAVFRKYGFDTLH"
     gene            3344..4669
                     /locus_tag="VNZ06_03280"
     CDS             3344..4669
                     /locus_tag="VNZ06_03280"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW19804.1"
                     /translation="MATSASLSAFRRRFSLDPTDLLRDPTYRRLWISILISQFGGQIT
                     LLALPLTAAVLLHASATQMGYLTAAEIFPFAFFSLPTGVWLDRVHKLPVYVMGELVIA
                     MAVTTVPIAWWLHWLSMSWLYVVGFSIGCVNTAAGSAAQVVLTQIVPRSRLVEAHAKN
                     ALAQSGAEVAGPGAAGALIKLTGAPFALLADALLLMSSASILRGVPLKETPSTDHPPF
                     WLAMRAGLSFVRHHQLLVAMAICVGSWQLFSQTAAVVQILFATRELGLGERSIGFCYV
                     ALGFGTVAASAIARDFGRRIGAGRTMVLGFGICASGWLLLSLAPPNAFGVAAYASMLL
                     FYGFGAVLIFVNFLALRQSVTPASLLGRMTTTMRWLILLPAGPGALLGGWIGEHVGLR
                     AALAVAGTGAMVLALFAWRVRIISSVMTLPGVKAEGVTPYSSDTAPSSE"
     gene            4732..5361
                     /gene="ccmA"
                     /locus_tag="VNZ06_03285"
     CDS             4732..5361
                     /gene="ccmA"
                     /locus_tag="VNZ06_03285"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HWW19805.1"
                     /translation="MNSAAKGLRADQVHIWRGDRHVLKGVSLSVSPGEMLHVSGVNGA
                     GKTTLLRVVCGLMRPEQGAVAWRGTSIHEDPMAYQADMAYASHEPALKADLTVLENLR
                     YAVGLKRSVSAPEIRSHLDRLGVASCADLPARVLSAGQRRRVSMARVVAMRASLWLLD
                     EPFANLDAPGCDLLLQLLDEHLAQGGLALVAAHQDIRLARAASHLELAA"
BASE COUNT         1004 a         1684 c         1719 g         1069 t
ORIGIN      
        1 acctcggccg acacgctgac gaaccaggag acgatccagg gtgcggggca catcggcaac
       61 aatcaaatga cgctcgtgaa ctcgggcacg atcgatgcga atcaattcgc cggcttgaac
      121 atcaccacca gcggcggcac aaccaacacc ggcaagctgg aggcgacggg cggatcgacg
      181 ctgaccctgg gcggcacgtt cacgaacacc ggcgggacga tttcggcgac cggcagtgag
      241 ttgcagctga cgggtgcgac ggtcagtggc ggtgcggtga cgctcaccgg cgcttcgctg
      301 ctgcagatga cgaattcgac gatccacggc ggcagcacgc tgacgaacag cacgacgggc
      361 acgatcgagg tcgtaggcgg atcgaacacg atcggcggca cgctgagcaa cccggcgggg
      421 ggcctgctga aggtcgataa cggcgcggtt ttgaacctgg agaacggctc ttacttgact
      481 tccggtgcga tgcagctgaa cgcggccggc agcacgacgg agctcgtggt tgccggggcg
      541 aacgtcacgc tgggcggcgg caccctgacg ctgtcgaaca acaccaacaa ctatatcttc
      601 ggagcgacct cggccgacac gctgacgaac caggagacga tccagggtgc ggggcacatc
      661 ggcaacaatc aaatgacgct cgtgaactcg ggcacgatcg atgcgaatca attcgccggc
      721 atgatcattc aaccgagcgg cggtttcacc aataatggaa ccctgttggt gggcagcggc
      781 atttttatgc atgtatccgg gggaccgttt agcaacttca gtggaaccac cctgacgggc
      841 ggcacctaca ataccggtgg acttctcgag atcgatcagt tgggcacagc cggcggcgag
      901 atcgtgacca acgccgccaa cattgtgctc aacggagcca actccagcat agttgatgcc
      961 ttcggcctcg acgctctgtc gaagctggcg gtcaccagcg cgggcggctc gttcagtctg
     1021 ctgggcggcc gaagctttac gaccgcgggg aactacacca acaacggcac gttgtcggtg
     1081 ggcaccggaa gcatattcag cgtccatagt gtgtttacga acttctcagg taccacgctg
     1141 acgggtggaa cgtataacgt gaccggcgcg tttcagtttg ccggcgccaa catcgtgacc
     1201 aattcggcca atatcaccct taccggtacg acgtcgaaaa tcctgaactc gacgacgagc
     1261 gccgacgggc ttgcgaactt cgccacgaat tcctcaaccg gcagcttcag tctcgcgggc
     1321 gcacgaaatt tcacgacggc tggcaacttc tcgaatgccg gcaatttctt gattggggca
     1381 gggagcgcgt tcaccgtggg tggaccgggt gctttcacgc agaccgcagg cacgacgact
     1441 gacaacggta cactgtcggc caccggtggt gtcagcctgg gcgggtcgct catcggcacc
     1501 ggatcggtta ccggaggtct cacatccaac ggtaacatca cccctgggac gtccacgacg
     1561 aaaacgggga tcctgaccga tactgggctg tacacgcaga attcggccgg tgttctgaat
     1621 atttcgattg gcggcacgac cgcgggatct acgttcgatg tgctaaattc cagcaccgca
     1681 aagctgggtg gcacgctgaa cgtccgggag gtcaacggtt tcgtaccgac catcggctcg
     1741 gcattcaaga ttctcaattt cacttcggaa accggccagt tcgcgaccgt caatgggtta
     1801 tccatcaacg gtaccgaaca tttcacgatc acttatgaag ggaccgatgt gctgctgaca
     1861 gtcgtgagcg gcgcagcagc cccggccaac gtgaccggca attttcgcaa tacggaccgg
     1921 atcgcgcgag cgatcgacac accggtcgat tttgccgacc gcacccggtc ctccgcccgg
     1981 tccggctttc atttagagca ggcttcacac ccaacgggcg ccccgacgcc gatcggcacc
     2041 agcctgcacg acgcgttggc gggacgatgg acgtccgcaa atccatctca tctcatttcc
     2101 accaagcagt ccgtcagcgc gctaccgcat tcatacgttg acggacgtgg acgactcgga
     2161 gcgaaagtgg ccaggggcaa cctgcaattt gcattaccgg gtttcaattc caggccgcag
     2221 ttatacttct cggtcaatta accagaatga tgctgaaggc aacgcgcgat gcgacctgcg
     2281 gcgtcaggag agccgtcgcg ggtcgctagc attagcacag catttggcct gcggtctttc
     2341 gtcttaaaat ccgttgatcg aaggatcatc ccggtccgac tccgggcggg ggcaccaatg
     2401 agtatttccc cgagccggcg cggttgcgtg atttgccccc tggtgcgtgc gctatgtaca
     2461 cttgaacatt acgtgcaggt aggagagatt cagtgaggac ggcaatgcgt ccgctgctgg
     2521 cgctgtggct cctggttttc ggtgtcgcca catttgcggc tgaagccacc aggcctgccg
     2581 tcatcgtgtt tggtgccgca tcgctgacca atgcgttgca ggagctgggg gacagctata
     2641 cgaaggagac ttccgttcct gtgaagtttt ccttcgcggc gagttccgtg ctggcgcgac
     2701 agatcgagag tggttcgccg gccgatatct tcttcgcggc cgatcttgac tggatggact
     2761 acctgcagtc ccgcaatctg atcaacaaga gttcgcggcg cgacgtgctg ggcaacaagc
     2821 tggtgttgat cgccccggtc gacagcaaga ttgagctcaa gatcgcgcct cattttgcac
     2881 tcgctgcagc gcttggcggc ggacggctgg cgacgggcga ccccgattcg gtacccgtgg
     2941 gccgctacgc gcgcagcgcg ctgacgaccc tcggggtgtg gaatgacgtg caggatcatc
     3001 tggtgcgcgc ggagaatgtt cgcggcgcgc tgatgttcgt tgaccgcaag gaggtgccgc
     3061 tcgggatcgt gtatgaaacc gacgcgctga tcgacaagca agtgcggatc gtcgacgtgt
     3121 tccccgcaaa ctctcattcg ccgatcacct acccgatcgc gttgacgact acggccgggc
     3181 cggaggcggc aaaatttgtt gactacctgc tgagtgccgc cggcgaagca gtgttcagga
     3241 aatacggctt cgacactctg cattgacgac cagcgccagc ccgttcgcgc gccgatcggg
     3301 gcaagagtta taatgcgcca atttgcaggt accggcgcaa accttggcaa cttcagcttc
     3361 cttgtccgca ttccgccggc gtttctccct cgacccgact gatctgctac gcgatccgac
     3421 ctaccgtcgc ctgtggatct cgatcctgat ctcgcagttc ggcggccaga tcacgctgct
     3481 cgcgctaccg ctgacggcgg ctgtgctgct acacgcgtca gcgacgcaga tgggctacct
     3541 gacggccgcc gagattttcc cgttcgcgtt cttttccctg cccaccggcg tgtggctcga
     3601 tcgggtccac aagcttccgg tctacgtcat gggagagctc gtgatcgcca tggcggtgac
     3661 gacggtgccg atcgcctggt ggctgcactg gctgtcgatg tcctggctgt atgtcgtcgg
     3721 cttctcgatc ggttgtgtca acacggccgc aggcagtgcc gcgcaggtcg tgctgacgca
     3781 gatcgtgccg cgttcacgac tcgtggaagc ccacgccaag aatgcgctgg cccagtccgg
     3841 cgctgaggtt gcaggtcccg gcgcggccgg cgcactgatc aaactgaccg gggcgccgtt
     3901 cgccttgctc gcagatgcgt tgctcctgat gagttcggcg tcgatcctgc gcggcgtacc
     3961 attaaaggaa actccgagca ccgaccatcc gccattctgg ctcgcgatgc gagcgggcct
     4021 cagtttcgtg cgccaccacc agctgctcgt cgcgatggca atctgtgtgg gcagctggca
     4081 gctgttcagc cagaccgccg cggtggtaca gatcctgttt gctacgcgcg agcttggact
     4141 cggcgaacgc tcgatcggct tttgctacgt cgcactcgga ttcggcaccg tggcggcgag
     4201 cgcgatcgcg cgcgacttcg gccgcaggat cggcgcgggg cgcacaatgg tgctcggatt
     4261 tggcatttgc gcgagcggct ggctcctgct gtcgctcgcg ccgccaaacg cgtttggagt
     4321 ggccgcgtac gcatcgatgc tgcttttcta tgggttcggg gcagtcctga tcttcgtcaa
     4381 cttcctggcg ctgcgccagt cggtcacgcc agcctcactg ctcgggcgca tgacgacgac
     4441 gatgcgctgg ctgatactgc tcccggccgg acccggcgca ttgcttggcg gctggatcgg
     4501 cgagcacgtg ggacttcgag cggcgctggc cgttgccggg accggcgcga tggtgctggc
     4561 actgttcgcc tggcgagtgc gcatcatcag cagtgtcatg acgctgccgg gcgtcaaggc
     4621 cgagggggtc accccctact cgtccgatac cgcgccatcg tcggaatgac atgcgaataa
     4681 tcacgcgaaa aggtagcgtt aactgattga tttcgctaaa ctctctggcc catgaacagc
     4741 gccgctaagg gtcttcgcgc cgatcaggtg cacatctggc gcggcgaccg gcatgtcctg
     4801 aagggcgtga gccttagcgt ctcaccgggc gaaatgctcc atgtcagtgg cgtgaacggc
     4861 gccggcaaga ccaccctgct gcgagtcgtg tgcggactga tgcgcccgga gcagggggcg
     4921 gtggcctggc gcggcacctc aatccacgag gatccgatgg cctaccaggc cgacatggcg
     4981 tacgcctcgc atgagccggc gctcaaggca gatctgacgg tgctcgagaa tctgcgctac
     5041 gccgtcggcc tgaaacgcag cgtcagcgcg cccgagatac gctcacacct cgatcggttg
     5101 ggtgtcgcct cctgtgccga cctgccagcg cgcgtgctgt cggcgggtca gcggcgtcgg
     5161 gtatcgatgg cgcgcgtcgt cgcgatgcgc gcctcgctgt ggctgctcga tgaacccttc
     5221 gcgaacctcg atgcgccggg atgcgacctt ctgttgcaac tgctcgatga gcatctcgcg
     5281 caaggaggcc ttgcgctggt cgctgcgcat caggacattc gcctggcacg cgccgcctcg
     5341 catcttgagc tcgccgcatg agtgtgaaca taagtcgtgc ggccggattg atcgtgcgcc
     5401 gtgaactgct gctcggtttt cgacgccccg atcaattgct gcagcccctg gtgtttttcc
     5461 tgatcgtcac gacaat
//
LOCUS       DATMRM010000113         5946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1624691, whole genome shotgun sequence.
ACCESSION   DATMRM010000113 DATMRM010000000
VERSION     DATMRM010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624691"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VNZ06_03290"
     CDS             <1..432
                     /locus_tag="VNZ06_03290"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HWW19806.1"
                     /translation="SMPEALSDRLRLVFDCLHRGDTPALIHCAAGKDRTGFAIAMVLD
                     ALGVSRTIILEDYLYTNQAVDLERFVLAHHPGLRRPQAGHPLGQLPTTIRQALLGAHP
                     EYLQAALAAVDARYGSTQGYLGQRLGIDADELKRMRDHLLE"
     gene            complement(456..935)
                     /locus_tag="VNZ06_03295"
     CDS             complement(456..935)
                     /locus_tag="VNZ06_03295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWW19807.1"
                     /translation="MTRVPTIRLALLTDAPAIARMSRLYVEHGLGWSWNVPRIEAAIR
                     CDSINVAVIRERHALSGFGIMQYGDQTAHLALLAVHPEWRKQGLATRLLAWLEKCADT
                     AGIGRIAVEARCDNPAALAFYQKQGYSQLARMPGYYRGRLDAIRFEKNLWVTSENNS"
     gene            complement(939..1292)
                     /locus_tag="VNZ06_03300"
     CDS             complement(939..1292)
                     /locus_tag="VNZ06_03300"
                     /inference="COORDINATES: protein motif:HMM:NF018008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWW19808.1"
                     /translation="MIPRVVRFDSSRVAAQEYFLPPEKLLSGNPQQRVWNHYTDASQK
                     FSAGMWHSEVGKWKISYTEEEYCQILEGTSVITDRDGHAVTVSAGESLVVPSGFVGTW
                     EVLTPTRKIYVIYEA"
     gene            1359..1574
                     /locus_tag="VNZ06_03305"
     CDS             1359..1574
                     /locus_tag="VNZ06_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19809.1"
                     /translation="MSFSSGRRGRTANSPPQLGHLPPSTCCAQRAQNVHSNEQMRASS
                     ESAGKSASQHSQFGLSCSMDGSGYGAV"
     gene            complement(1559..2089)
                     /locus_tag="VNZ06_03310"
     CDS             complement(1559..2089)
                     /locus_tag="VNZ06_03310"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HWW19810.1"
                     /translation="MRPHIAERLRVLACAALFASAAATSGNEDATVAATLKPPAGEVL
                     KLKLVGNGVQIYQCQQGAWKFQAPDAKLSDETGHVVGKHYAGPTWESADGSKIVGEVK
                     AHEDSPDSRAIPWLLLGVKSASGNGVFGNIRSIQRLQTSGGQAPHRSCDASHANQSAR
                     VPYSAHYYFYYFYTAP"
     gene            complement(2202..2627)
                     /locus_tag="VNZ06_03315"
     CDS             complement(2202..2627)
                     /locus_tag="VNZ06_03315"
                     /inference="COORDINATES: protein motif:HMM:NF018404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HWW19811.1"
                     /translation="MISLEIIVNGQRRAIAGVAEAELLTATVSLYPAVKDGWLDVSGA
                     VLPADQPSADANWLSAALTMGDRVEVRLVESEQPQPPKLSRTDPTAQASDGIPTVCAF
                     CEKTYLQVEGMMSSRKAMICRGCVEYLHEMMNPEAHTAD"
     gene            complement(2624..3301)
                     /gene="lipB"
                     /locus_tag="VNZ06_03320"
     CDS             complement(2624..3301)
                     /gene="lipB"
                     /locus_tag="VNZ06_03320"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HWW19812.1"
                     /translation="MAGAPEAAEVVTTPVLKWLGRVHYEPTWRAMQRFTEQRDPWTRD
                     EIWLLEHEPVFTLGMNADRSHVLSPGDIPVVQIDRGGEVTYHGPGQLVVYALIDVRRA
                     GLGVRDLVTALERSVISYLARYDVAAQTQPSAPGVYVCGRKIASVGLRIRRNGSYHGL
                     AFNVRMNLEPFQRINPCGRAGLEMTQFSDFHRDADVAHLAPELGAVFVSTLSQLRQSG
                     CETKGQN"
     gene            complement(3313..4347)
                     /gene="lipA"
                     /locus_tag="VNZ06_03325"
     CDS             complement(3313..4347)
                     /gene="lipA"
                     /locus_tag="VNZ06_03325"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HWW19813.1"
                     /translation="MDSFKGIPVIGDTPVRSGQKYVTPQGFTAIKDGVKQGQSVPAGR
                     PLTGKPRWLKAPLAAGVGYDFVRRTVREHRLSTVCEEAKCPNIGECWNAGTATVMLMG
                     AVCTRACRFCAVDTGNPRGWLDAEEPQNVADSVALMQLKYVVLTSVNRDDLGDGGAAH
                     YADCIRAIKRRNPQTAVEALTPDFQGVLPHVELVLDCEPEVFAQNVETVRRLTHPVRD
                     PRAGYEQTLSVLAHAKRYRPQTLTKTSLMLGLGETDGEIIECMDHLRRIGVDILTLGQ
                     YLRPTINHLAVERFVTPAQFEQYREAALTRGFRECVAGPLVRSSYRAEQALVGNNVGL
                     NLRPLAASES"
     gene            complement(4408..5067)
                     /locus_tag="VNZ06_03330"
     CDS             complement(4408..5067)
                     /locus_tag="VNZ06_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWW19814.1"
                     /translation="MTVRRAMILAAGRGERMRPLTDRTPKPLLEVRGKPLIVYHLERL
                     CALGLSEVVINLAWLGNVIRERLGDGARWNLHIRYSDEGAEPLETGGGVFKALPWLGP
                     DPFLIVNGDVFTDYDFAALRIAPEAWAQLVLVPNPPQGLHGDFSLRDGFVVDEPGAPR
                     WTYSGIGLYRPELFTGCVAGRFKMLPVFQRAIIDRRLQGEIYTGLWNDVGTADRLSAL
                     Q"
     gene            complement(5064..>5946)
                     /locus_tag="VNZ06_03335"
     CDS             complement(5064..>5946)
                     /locus_tag="VNZ06_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWW19815.1"
                     /translation="KEDIGPFLQVAALLERCGVHVPHVEAADGPRGMVLLEDLGSVPY
                     LTRLRAGADPQSLYGDALQALYRIQVGGAEASRLLPPYDAAALEREMELMPVWFCERH
                     LALRLDDSDRALLARSFEFLTAEALAQPVVFVHRDFHSRNLMVLPERNPGVIDFQDAL
                     VGPVGYDLVSLLKDCYIDWPRSRIEYWVGDYRTQLRQTPAIGRVLGGSSEREFLRWFD
                     LIGVQRHIKVLGIFARLWYRDGKNGYLGDLPRTLNYVREATALHPELQGLGQWIERRL
                     MPEFANANARALASSAA"
BASE COUNT         1097 a         1989 c         1808 g         1052 t
ORIGIN      
        1 agcatgcccg aggcactatc ggatcgcctg cgcctcgtgt tcgactgcct gcaccggggt
       61 gacactccgg cgctgattca ttgcgccgcc ggcaaggacc ggaccggctt tgcgatcgcg
      121 atggtcctgg atgcgcttgg cgtgtcgcgc acgatcatcc tcgaggacta tctctatacg
      181 aaccaggccg tagatctgga gcgctttgta ctggcgcatc accccggcct gcgacgcccg
      241 caagccgggc atcctctcgg gcagttgccg accacgatac gccaggcatt gctcggcgcg
      301 caccccgagt acttgcaagc agccctggcc gccgtagacg cacgctacgg ctcgacgcag
      361 ggctatctgg ggcaacggct cggtatcgat gccgacgagc taaagcggat gcgcgaccac
      421 ctgctggagt agacggcctc acgcagcgcc agccgttagc tgttgttctc gctcgtcacc
      481 cacagattct tctcgaaacg gattgcatcg agccggcccc ggtagtatcc gggcattcgc
      541 gctagctgcg agtaaccctg cttctgatag aacgcgaggg ccgcgggatt gtcgcacctc
      601 gcctcaaccg cgatgcgtcc aataccggca gtgtcggcac acttttccag ccatgccagc
      661 agccgcgtgg caagcccctg cttgcgccac tcgggatgca ctgccagcag cgcgagatgc
      721 gcggtctgat cgccgtactg catgatgcca aaaccgctca gagcatgccg ctcgcgaatg
      781 accgccacat tgatcgagtc gcaacggata gcggcctcga tgcgtggcac gttccagctc
      841 cagcccaggc catgctcgac gtatagacgc gacatgcgag cgatcgccgg cgcatcggtc
      901 aacagggcca gccgtatggt cggcacccgt gtcatcgact aggcctcata gatgacgtag
      961 atcttgcgtg tcggtgtgag cacttcccag gtaccgacga atccgctcgg cacgaccagg
     1021 ctctcgcccg ccgacaccgt cacggcgtgt ccgtcacgat cggtgatgac actcgtaccc
     1081 tcgagaatct ggcaatactc ctcctcggta tacgaaatct tccacttgcc gacctcgctg
     1141 tgccacatcc cggcggagaa cttctgcgaa gcgtcggtgt agtggttcca gacgcgttgc
     1201 tgcggattgc cggacagcag tttctccggc ggcagaaagt attcctgcgc ggcgactcgc
     1261 gacgaatcga acctgacgac cctgggaatc atggctgtcg ggcagcgcca gcgcagcctt
     1321 tcggtttata gatccgctcg gccgatgccg gatactgcat gagtttttcc tccggccgcc
     1381 gcggacgcac cgccaactca ccgccgcagt tggggcattt gccgccgagc acttgctgcg
     1441 cgcagcgcgc gcagaacgtg cactcgaacg agcaaatgcg cgcgtcgagc gaatccgccg
     1501 gcaaatcggc gtcgcagcat tcgcagttcg gtctgagttg cagcatggat ggcagcggct
     1561 acggggccgt gtagaaatag tagaaataat agtgagcgct atagggaacc cgcgcgctct
     1621 ggttggcgtg gctcgcatcg cagctgcgat gtggcgcctg cccgccactg gtctgcagcc
     1681 gctggatact tcggatattg ccgaacacgc cgttgccgct ggccgacttg acaccgagca
     1741 acagccaagg aatagcgcgc gagtccggac tgtcctcgtg cgccttgacc tcaccgacga
     1801 tcttgctgcc atcagccgac tcccaggtcg ggcctgcata gtgtttgccg accacatgcc
     1861 cggtctcatc gctcaacttg gcatccgggg cctgaaactt ccacgcgccc tgctgacact
     1921 gatagatctg cacgccattg ccgacgagct tgagcttcaa cacctcgccg gcgggcggct
     1981 tcaacgtcgc cgctaccgtg gcatcctcat tgcctgaggt cgccgccgcg ctggcaaaca
     2041 gcgcagcgca ggccaacacc cttaagcgct cagcgatgtg aggcctcatg ttatcgcgta
     2101 ccttgctgtg gcaattcgcc gatcgacagc catgtcagtt cttcgtccga gaagtcgcgc
     2161 tgcgccatcg cccaacgcgc acgatgtaga tgcgccgcat cttaatcggc tgtatgcgct
     2221 tcggggttca tcatttcgtg caggtactcc acacagccac ggcagatcat cgccttgcgc
     2281 gatgacatca tgccttcgac ctgcagatag gtcttttcgc aaaacgcaca caccgtggga
     2341 atgccatcgc tggcctgcgc ggtcggatca gttcggctca gtttcggcgg ctgtggctgc
     2401 tcagattcca ccagacgcac ctcgacacga tcgcccatcg tcagcgccgc cgacagccag
     2461 ttggcatcgg ccgatggctg atccgcgggc agcacggcgc cggacacgtc cagccagcca
     2521 tctttcacgg ccgggtatag cgagacggtt gcggtgagca gctcggcctc agcaacgccc
     2581 gcgattgcgc ggcgctgacc attcacaatg atttccaggc tgatcaattc tgccccttcg
     2641 tttcgcagcc tgattgtcgt agctgcgaca acgtgctgac gaatacggcg cccagttcag
     2701 gggccagatg agcgacgtcg gcgtcgcggt ggaagtcgga gaactgcgtc atctccaggc
     2761 ctgcgcggcc acaggggttg atgcgctgga acggctcgag gttcatgcgg acattgaatg
     2821 ccagtccatg atagctgcca ttgcgacgaa ttctcagccc gacgctggca atcttgcgcc
     2881 cacagacata cacgcccgga gcgctgggtt gggtctgtgc cgcgacatcg tagcgcgcca
     2941 gataggaaat cacgctgcgc tcgagcgccg tcaccaggtc gcgcacccca agccctgcgc
     3001 gccggacatc gatcagggca tacaccacga gctggcccgg gccgtgatac gtcacctctc
     3061 cgccccgatc gatctgaacg accggtatgt cgcccgggga cagcacgtgc gagcggtcag
     3121 cgttcatccc cagagtgaac actggctcgt gctcgagcag ccagatctca tccctggtcc
     3181 agggatcgcg ctgttcggtg aagcgctgca tggcgcgcca ggtgggctcg tagtgtacgc
     3241 ggcccagcca tttcaggacc ggtgtcgtca cgacctcggc agcctccggc gcacccgcca
     3301 tgactgccgc gttcacgatt cggaagccgc cagcggcctc aggttgagac cgacattgtt
     3361 gccgaccagg gcctgttcgg cgcgatagct ggagcggacc agcggacctg cgacgcactc
     3421 gcgaaagccg cgcgtcaggg cggcctcgcg atactgttcg aattgcgccg gtgtcacgaa
     3481 tcgctccacc gcaagatggt tgatcgtcgg gcgcaaatac tgtccgagcg tcaggatgtc
     3541 gacgccgatg cggcgaagat ggtccatgca ctcgatgatc tcgccgtcgg tttcacccaa
     3601 ccccagcatc aggctggtct tggtcagcgt ctgcggccga tagcgtttag cgtgcgccag
     3661 cacggacaag gtctgctcgt agccggcgcg cggatcacgc acggggtggg tgagacggcg
     3721 cacggtctcg acgttctgcg cgaagacctc cggctcgcaa tcaagcacca gctccacgtg
     3781 cggcaacaca ccctggaaat ccggtgtcag agcctccacc gcggtctggg gattgcgccg
     3841 cttgatcgcg cggatgcagt cggcgtagtg cgcggcgccg ccatcgccaa gatcgtcgcg
     3901 attgaccgac gtcagcacca cgtacttgag ctgcatcagc gcaacactgt ccgcgacatt
     3961 ctgcggctct tcggcatcga gccagccgcg cggattgccg gtatccacgg cgcagaagcg
     4021 gcaggcgcgc gtgcacacgg cgcccatcag catcacggtt gcggtgccgg cattccagca
     4081 ttcaccgatg ttcggacact tggcctcctc gcagaccgtg gacagccggt gctcacgcac
     4141 ggtacgccgc acgaagtcgt agccgacgcc ggctgcgagc ggggccttca gccatcgggg
     4201 tttgccggtc agcgggcggc cggcgggcac cgactggcct tgcttgacgc cgtccttgat
     4261 cgctgtaaat ccctgtggcg tcacgtattt ctggccgcta cggaccgggg tgtcgccgat
     4321 caccggaatg cctttgaagc tgtccatcag gcgtttaatt ttaccggaag ccactcggcg
     4381 gcgcctcaag agacggcggg tgggcggcta ttgcagggcg ctcagccgat ccgcggtgcc
     4441 gacatcgttc cagaggccgg tatagatctc gccctgcaga cgccggtcga tgatcgcgcg
     4501 ctgaaacact ggcagcatct tgaacctgcc cgcgacgcag cccgtgaaca gctctgggcg
     4561 gtacaaacca attcccgaat aggtccagcg cggcgcaccc ggttcatcca cgacgaagcc
     4621 gtcgcgcagc gaaaagtccc cgtgcagccc ctgcggggga ttgggcacca gcaccagctg
     4681 agcccaggcc tcgggcgcga tccgcaacgc cgcgaagtcg tagtccgtga acacatcgcc
     4741 attgacgatc agaaacggat cgggtcccag ccagggcagc gccttgaaga caccgccgcc
     4801 ggtctcgagc ggctcggccc cctcatcgct gtagcggatg tgaagattcc atcgcgcccc
     4861 gtccccgagg cgctcgcgaa tcacattccc cagccacgcc agattgatga ccacttcgct
     4921 cagaccgagc gcgcagagtc gctccaggtg atagacgatc aggggtttgc cgcgtacctc
     4981 cagtaacggt ttgggcgtgc ggtcggtcag cggccgcatg cgctccccgc gtccggctgc
     5041 caggatcatt gcgcgacgca ctgtcatgcc gcgctcgatg ccagcgcacg cgcgttggcg
     5101 ttcgcgaact ccggcatgag gcgccgctcg atccactgcc cgagcccctg cagctcgggg
     5161 tgaagcgccg tcgcctcgcg aacgtagttc aatgtgcgcg gcagatctcc caggtagccg
     5221 ttcttgccat cgcgatacca cagacgcgca aaaatgccga gcaccttgat gtgccgctgg
     5281 acgccgatca gatcgaacca gcgaagaaac tcccgttcac tcgagccgcc cagcacacgg
     5341 ccgatcgccg gcgtctgacg cagttgcgtg cgatagtcgc cgacccagta ctcgatgcgc
     5401 gaacgaggcc agtcgatgta gcagtccttc aacagcgaca ccaggtcgta accgacggga
     5461 cccaccaacg cgtcctgaaa atcgataacg cccggattgc gctcgggcaa caccatcaga
     5521 ttgcgcgaat gaaagtcccg gtgcacgaac accaccggct gtgccaatgc ttcggcggtc
     5581 agaaattcaa agctgcgtgc cagcagcgcg cgatcggagt catccaggcg cagtgccaga
     5641 tggcgctcgc agaaccagac cggcatcagt tccatctcac gctcgagtgc cgcggcatca
     5701 tagggcggca gcagtcgcga ggcctcggcg ccccctactt gaatgcgata gagcgcctgc
     5761 agcgcatccc cataaaggga ctgcggatcg gcgccggcgc gcagccgggt cagatagggc
     5821 actgagccga ggtcctcgag cagcaccata ccccgtggcc cgtcggcggc ctcgacatgt
     5881 ggcacgtgca cgccacagcg ttcgagcaga gccgccacct gcagaaacgg gccgatgtct
     5941 tccttg
//
LOCUS       DATMRM010000114         7248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2842655, whole genome shotgun sequence.
ACCESSION   DATMRM010000114 DATMRM010000000
VERSION     DATMRM010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2842655"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1216)
                     /locus_tag="VNZ06_03340"
     CDS             complement(212..1216)
                     /locus_tag="VNZ06_03340"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWW19816.1"
                     /translation="MKSFPAFKLVRMALAIGALVALWLKVQRTHPLERLAGARLEFIA
                     LAVVVVILDGMARAWNWRQLIRSMHVAPTVGYGMVLRLFWSGAFLGQVVPSTAGTDAL
                     RAILASRAFGGRMSEHAAAVVVLNIVSLAAGCAVGLGVAAWLFATDPQHGVRPILVLL
                     FAAALGAALILYYLVHSQRGVLLWVLRRMRGPAMKVRRPLRRFTQRLIVLERHHIGLL
                     PILAVAVLTLVTRATMFGLIGYATGIVLPAGVWASLVPGNMLSGLIPYSVAGYGGDQA
                     AMVYFVTAFGISAGAAVAFALILPLMAMTFNMLGGLFVMSDHGASRSAIQVPERPAAD
                     "
     gene            complement(1216..2193)
                     /locus_tag="VNZ06_03345"
     CDS             complement(1216..2193)
                     /locus_tag="VNZ06_03345"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWW19817.1"
                     /translation="MKVLVTGATGFTGGHLARELQRRGRQVVALVRDPSKAQVLTQDG
                     IELSAGDLTDAQSLRRAAQGCEVVYHIAAVYREARHGDDFYRLVNVHGTDNVVQAVRD
                     AGVGRMVHCSTVGVHGDIANCPADESAPFGPGDIYQQTKLEGEQLVAKYMASGFPGVI
                     FRPVGIYGPGDRRFLKLFKTVYRGQFRMIGSGGVLYHMTYIDDLVDGIIRCGEQSGAV
                     GHTYILSGPRYTTIDVLVETIARVSGRRPPKGRIPLAPVMAAATLCEWLCKPLRIEPP
                     LHRRRLDVFTKDRGFTSAKAHREIGYEARVDLEEGLTRTYQWYLANGLL"
     gene            complement(2190..3674)
                     /locus_tag="VNZ06_03350"
     CDS             complement(2190..3674)
                     /locus_tag="VNZ06_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWW19818.1"
                     /translation="MARSDHDLLVVGGGVYGVALLLEAARRGLKAALVERNDFGSGTS
                     SNSLRILHGGFRYLQSVDLVRMAQSVNERRRWARCFPELVRPLTCVMPLYADGLKRAS
                     VVRVALVMNDLLSASRNAGVLPALHIPRGRIADVVEVGRICPLIRTAGLEGAALWTDY
                     RALSSERLLIEMLRWAVQMGAQAANYTAAVELMTDGGRVIGVRARDAEANREHEIRAS
                     VVVNCTGPESRLLSARWDREVVDLFRPSLAFNVLIDRELPSRHALGVAAPQAGAPVLF
                     LVPCGGGVLAGTWHLPRPDQTTAAAPSEQELQQFIDSVSAALPTWGVSIKHVRRVFAG
                     LLPVTNSDTVNLSGREELVDHGSRGGPVGLVSLSGVKFTTARATAQRAMKLILRLLRR
                     TAPQPEEDVPRTVAPGTTLLTDASRLKGEGAGSSAEWLRRCVREESVRCLDDLLLRRT
                     NWVTTTADMAELAGQVSAMIGDLPGHAHTATGAADSRRYGEIGS"
     gene            complement(3695..5578)
                     /gene="asnB"
                     /locus_tag="VNZ06_03355"
     CDS             complement(3695..5578)
                     /gene="asnB"
                     /locus_tag="VNZ06_03355"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HWW19819.1"
                     /translation="MCGIAGIVALAGAAPDSATIQAMCDAIRHRGPDDAGYFVEGPVA
                     LGMRRLAIIDLVTGHQPIFNEDRTACIVFNGEIYNYRELRDQLIAAGHRFATHSDTEV
                     IIHLWEQYGTEFAERLNGMFAVAIWDLRARKLLLARDHVGIKPLYYARGSDSFVFGSE
                     VKALLACGRVGSTLEIDALGQFLAWEYVPGTRTMMKEIRRLGAGQMLELDLATGKLSF
                     ARFWRVPQPVQASARARSPKEWADELDATIAAAVRRQMIADVPLGAFLSGGVDSSLVV
                     HAMGSAHSFSIGFEDPSYDETQWSKRVAEHLGVNHEIEIIQPQVRELFELLMQGMDDP
                     IGDFSIFPTYLVSRLARRQVTVALSGDGGDELFGGYDTYDAQQRSRLWRKLPLFLRSG
                     LIEPLTQALPPSAAKKGLVNKAKRFVEGAALDERLGHARWRLFAADSLIKGLLTPMAR
                     AEMHTPIGDHILALHDEAGMLDERDKALYVDLRSYLVDNCLIKVDRMSMLCSLEVRVP
                     LLDREVVDLAFRMPSNLKYRAGETKILLKQVAARHVPRECVYRSKQGFSIPIKNWLKE
                     QFRPLLNEYLDRRRVAREGIFNPETIERLRAEHDANIANHSHLLWGLLMFSKWRELWK
                     VTA"
     gene            complement(5579..6772)
                     /locus_tag="VNZ06_03360"
     CDS             complement(5579..6772)
                     /locus_tag="VNZ06_03360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW19820.1"
                     /translation="MKALVIAPQPFFTPRGTPFSVYYRTLVLAENGVDIDLLTYGQGA
                     DVDLPRTRVVRIPSMRWLGKVSVGPSIKKAFLDIFMIAWTLGLLIRHRYQVVHAHEEA
                     VFWCRFLKPIFRFRLIYDMHSSLPQQLTNFDYTRSRALIGLFNTLENSCLHAADAVIT
                     ICPDLSDYALKVGVDPSKHLLIENCIFDDVKLRSNGDEGLVTNHDLELQLIARLSQDK
                     RPRVVYAGTFEPYQGIEVLVKAFAGVVEVVPTAQLVLAGGTPEQIQAMRKLAVDHKVD
                     RSIEFVGTVSKRAAVQLLKLATVLVSPRMHGTNTPLKIYELLASGIPLVATRIWSHTQ
                     VLNDEVCILVDANAEAIAQGMITALQDKVRVESIARGAQALYESRYTRSIYVQKIRQL
                     ISLVA"
     gene            complement(6769..>7248)
                     /locus_tag="VNZ06_03365"
     CDS             complement(6769..>7248)
                     /locus_tag="VNZ06_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19821.1"
                     /translation="EIVPDGLAMRPTLERVAASGAFPVIPWGFGKWWLRRGELVRRQL
                     TLFAPQSLALGDNGGRLANAPRPTLFQRAEQQGFAVLPGTDPFPFPGQEARVGSYGFV
                     LENWHRSDQPVEELLQRLRALSGTPRCFGRLTNPLRFAISQVRMQVRMRTPAKQIAP"
BASE COUNT         1522 a         2376 c         2084 g         1266 t
ORIGIN      
        1 gttgaggaag taggggtgaa tccgcacacg cgcgggatcc acacgcggga acacattcat
       61 gtacagacgg ccaccagcat gcaagctgcc gtcaaagcag acgtgatcga tgagccacag
      121 cacattgaga ggtggagttg acatagttct tgggtccttg cgggaatcac atgatacgta
      181 gcgcccggaa tagaatcgag acggccttca cttaatcggc ggcagggcgt tcaggcacct
      241 gaatcgccga tcgtgaggct ccgtgatccg acatcacgaa caatccgccc aacatgttaa
      301 aggtcatggc catcagcggc aggatcagcg caaaggcgac ggccgccccg gcgctgatgc
      361 caaacgccgt gacaaagtac accatcgcgg cctgatcgcc cccatagcct gcgaccgaat
      421 agggaatgag cccggagagc atgtttccag gcaccaggga ggcccatacg ccggcaggca
      481 gcacgattcc ggtcgcgtag ccgatcaggc cgaacatcgt ggcgcgcgtc accagcgtta
      541 gcacggcgac cgccaggatt ggaagcagcc cgatgtgatg ccgctccagg acgatcagcc
      601 gttgggtgaa gcgccgcagc ggccggcgaa ccttcatggc cgggccgcgc atccggcgga
      661 gcacccatag caatacccca cgctggctgt ggaccaggta ataaagaatc agtgccgcgc
      721 caagcgccgc agcaaatagc aggacgagaa tcggtcgcac gccgtgttgc ggatcggtcg
      781 caaacagcca cgcggcgacc cctaggccga cagcgcaacc cgcggccagg ctgacaatgt
      841 tgagtaccac caccgcggct gcgtgctcgg acatgcgacc gccgaacgcg cgcgaagcca
      901 ggatcgcgcg cagtgcgtca gttcccgcgg tggacggcac cacctgtccc aggaaagcgc
      961 ccgaccagaa caggcgcagc accataccgt acccaaccgt cggagccacg tgcatcgaac
     1021 gtatcagctg acgccagttc caggctctgg ccatgccatc caggatgacc acaaccacgg
     1081 ccagcgcgat gaattcgagc cgcgctccgg cgagtcgttc aagcggatgg gtccgctgca
     1141 ctttgagcca aagcgcaacc aacgctccga tcgccaacgc catgcgcacc agtttgaaag
     1201 ccggaaacga tttcatcaga gcaagccgtt cgccagatac cactgatagg tccgtgtgag
     1261 accctcttcg agatccacac gtgcttcata tccgatttcg cgatgggctt tggccgaagt
     1321 aaacccacgg tccttggtga agacatccag ccgccgccga tgcagtggcg gctcgatgcg
     1381 cagcggcttg cacaaccact cacacagggt cgcggcggcc atcaccggcg cgagcggaat
     1441 cctacctttg ggcgggcgac gacccgacac gcgcgcgatc gtttccacca ggacatcgat
     1501 cgtggtatag cgtggaccgg agagaatgta agtatgtccg accgcacctg actgttcgcc
     1561 acaacgaatg attccgtcca ccagatcgtc aatgtaggtc atgtgataca ggacaccgcc
     1621 cgagccgatc atgcggaatt gaccacgata aacggtcttg aagagcttta gaaaccgacg
     1681 gtcacccggc ccgtagatgc caacgggacg aaagatgacg cccggaaatc ccgacgccat
     1741 gtacttggcg accagttgtt caccttccag tttggtttgc tggtagatat ccccggggcc
     1801 aaacggcgcg ctttcatccg ccgggcagtt ggcgatgtca ccatgcacac cgactgtgct
     1861 gcaatggacc atgcggccga cgccggcatc gcgcaccgcc tgcacgacgt tatcggtccc
     1921 atgcacattc accagccgat agaagtcatc tccgtggcga gcctcgcgat aaaccgcagc
     1981 gatgtgatac accacctcac agccctgagc ggcacgccgc agcgactggg catcggtcag
     2041 atcacctgct gacagctcga tgccgtcctg cgtcaacacc tgtgctttgg agggatcgcg
     2101 cacgagcgcc accacctgtc ggccgcgacg ctgcaactcg cgcgccaaat ggccgccggt
     2161 aaagcccgtc gcgcctgtca ccagtacctt catgagccga tttctccata gcgccgcgaa
     2221 tccgcagcgc cagtcgccgt atgcgcatgc ccgggcagat cgccaatcat tgccgacacc
     2281 tggccggcga gttcagccat atcggcggtg gtcgtcaccc agttcgttcg ccgcagcagc
     2341 agatcatcca ggcagcgaac ggactcttcg cgcacacagc gtcgcagcca ctccgccgat
     2401 gatcccgcac cttcaccttt gagtcggctc gcgtcagtca gcagcgtggt gccgggcgcc
     2461 acggttcgcg gcacatcctc ctccggctgc ggcgctgtcc ggcgcaatag tcgcaggatc
     2521 agcttcattg cccgctgtgc agtcgcacgg gcggtggtga atttgacccc cgacaagctg
     2581 accagcccca ccgggccgcc gcgactgcca tggtccacca actcctcacg tccgctcaga
     2641 ttcaccgtgt cgctgttcgt cactggcagc agcccggcga atacgcgacg cacatgcttg
     2701 atggagacac cccacgttgg aagcgccgcg ctcacgctat cgatgaactg ttgcaactcc
     2761 tgctcgctcg gggcggctgc agtggtttga tcaggacgag gcaggtgcca ggtgcccgca
     2821 aggactccac ccccgcatgg caccagaaac agcaccggtg cgccagcctg cggggctgcg
     2881 acaccgagcg catgtcggct cggtagttcg cgatcgatca gcacgttgaa cgccagcgac
     2941 ggacgaaaca ggtccaccac ctcccgatcc cacctggccg agagcagccg cgactccgga
     3001 ccggtgcagt tcactaccac ggaagcacgg atttcgtgct cgcgattggc ctccgcatcg
     3061 cgcgcccgta cgccaatgac acgcccgcca tccgtcatca gctcgaccgc ggccgtatag
     3121 ttggcagcct gtgcgcccat ctgtaccgcc cagcgaagca tctcgatcag cagtcgctcg
     3181 gagctcaggg cgcgatagtc agtccaaaga gcggctccct cgagcccagc ggttcggatc
     3241 agcggacaga tccgtccgac ctcgacgacg tcagcgatgc ggccgcgggg tatgtgaagc
     3301 gcaggcaaga ccccggcgtt ccgggaagcc gacagcaggt cattcatcac caacgcaacg
     3361 cgcacaaccg aggcgcgctt gagcccgtcc gcatacagag gcatgacgca ggtcagcggc
     3421 cgcaccagtt ccgggaagca acgggcccaa cggcgccgtt cgttgactga ctgcgccata
     3481 cgcaccagat cgactgattg cagatagcga aagccaccgt gcaggatgcg cagcgaattc
     3541 gagctggtac cgctgccaaa gtcattgcgc tccaccagtg cggccttcaa cccgcggcgc
     3601 gcggcctcca gcaatagcgc cactccatag acaccgccac cgacgacgag caggtcgtga
     3661 tcggatctgg ccatgccgga tggatcgcgg acgatcaagc ggtaaccttc cagagctcgc
     3721 gccactttga aaacatcagc aatccccaca acaggtggct gtggttggcg atgtttgcgt
     3781 catgttcggc acgaagacgc tcgatggtct ccggattgaa gataccctcg cgcgccacac
     3841 gccttcgatc gaggtattca ttcagcagag gcctgaactg ctccttgagc cagttcttga
     3901 tcgggatgct gaacccttgc ttgctacggt atacgcattc gcgcggcaca tgtcgggctg
     3961 cgacctgctt gagcaggatc ttggtctcgc cggctcgata cttcagattc gatggcatcc
     4021 gaaatgccaa gtcgaccact tcgcggtcga gcaacggtac gcgtacttcg agcgagcaca
     4081 gcatcgacat gcggtctacc ttgatcaggc agttgtcgac caggtagctg cgaagatcca
     4141 cgtaaagcgc cttgtcgcgc tcatccaaca tgcccgcctc gtcgtgaagc gccaggatgt
     4201 gatcgccgat cggcgtatgc atttccgccc gcgccatcgg tgtcagcaat cccttgatca
     4261 atgagtcggc agcaaacagt cgccaccggg cgtggccgag acgctcgtcc agagccgctc
     4321 cttcaacgaa gcgctttgcc ttgttgacga gccccttctt cgcggcgctg ggtggcaagg
     4381 cctgcgtcag cggctcgatc agcccagaac gcaggaacag cggcagcttg cgccatagcc
     4441 gagagcgctg ttgcgcatcg taggtgtcat agccaccgaa caactcatcg cctccatcac
     4501 ccgagagcgc aacggttacc tgccggcgcg caagcctgga gaccagatag gtcggaaata
     4561 tcgagaaatc tccgatcgga tcgtccatcc cctgcatcag cagttcaaat agttcgcgca
     4621 cttgcggctg gatgatctcg atctcatgat tcactcccag gtgttcggca acgcgtttcg
     4681 accattgggt ctcatcgtag ctaggatctt caaatccgat actgaatgaa tgcgcgctac
     4741 ccatggcgtg cacgaccagc gaactatcca ctcctccaga cagaaatgca cccagcggca
     4801 catccgcaat catctgtcgg cggaccgccg ctgcgatcgt tgcatcgagc tcatccgccc
     4861 attcctttgg cgaccgagca cgcgcgcttg cctgcaccgg ctgcggcacc cgccagaatc
     4921 tcgcaaaaga cagtttgccg gtggcaagat ccagttccag catttgcccg gctcccaagc
     4981 ggcgaatctc cttcatcatc gtgcgcgtgc caggcacgta ctcccaggcc aggaactggc
     5041 caagcgcatc gatttccagc gtgctgccca ctcgaccgca agccagcagc gctttgactt
     5101 ccgatccgaa cacgaaacta tcgctgccgc gggcatagta cagcggcttg atacccacgt
     5161 gatctcgtgc gagcagcagt ttgcgcgctc tcagatccca gatcgcgacc gcaaacatgc
     5221 cattcaggcg ctcggcgaac tccgtcccat attgctccca caggtgaatg atcacctcgg
     5281 tatcggagtg cgttgcgaaa cggtgccctg ccgcaatcag ctgatcgcgc agttcgcgat
     5341 agttgtagat ctcaccattg aaaacaatgc aagccgtccg atcttcattg aagatcggct
     5401 gatggcccgt taccaggtcg atgatcgcca ggcggcgcat gcccagcgcc accgggcctt
     5461 cgacgaagta accggcatca tccggtccac gatgcctgat cgcatcgcac atagcctgga
     5521 tcgtggcgct atccggtgcg gcgcctgcaa gtgccacgat tccggcgata ccacacatct
     5581 aagcgaccaa cgaaatcagc tgccggattt tctggacgta gatggaccgc gtgtagcggg
     5641 actcgtacaa ggcctgtgcg ccgcgcgcga tcgactcgac acgcacctta tcctgcaggg
     5701 ccgtgatcat accctgagca atcgcctcgg cattcgcatc gaccagaatg caaacctcgt
     5761 cgttgagcac ctgggtatgc gaccagatgc gcgtcgcaac cagcgggatg ccgctggcga
     5821 gcaactcgta aatcttcagc ggagtattgg tgccatgcat gcgcggtgat accaacacgg
     5881 tggcgagttt cagcagctgc acggccgccc gcttgctgac ggtacccaca aactcgatcg
     5941 accgatcgac cttgtggtcc accgccagct tgcgcatggc ctggatctgc tccggcgtgc
     6001 cgccggcaag caccaactgc gccgtcggca cgacttcgac cacgccggca aaggctttga
     6061 ccagcacctc gatgccctga tagggctcaa aggtgccggc gtacacgacc cggggtcgct
     6121 tgtcctgcga cagacgcgca atcagttgga gttccaaatc gtgattggtg accagtcctt
     6181 cgtcgccgtt gcttcgcagc ttcacgtcat caaaaataca attctcgatc aacagatgct
     6241 ttgacggatc gaccccaacc ttcagtgcat agtcgctgag atcggggcaa atcgtgatga
     6301 ccgcatcggc cgcgtgcaga cagctgttct cgagcgtatt gaacagaccg atgagtgcac
     6361 gcgagcgtgt gtagtcgaag tttgtgagct gctgcggcag gctcgaatgc atgtcgtaga
     6421 tgaggcggaa cctgaagatc ggcttcagga agcggcacca gaacaccgcc tcttcgtgcg
     6481 catgcactac ttggtagcgg tgccggatca gcagccccag ggtccaggca atcatgaaga
     6541 tatcgaggaa cgccttcttg atcgatgggc ccaccgacac tttgccgagc catcgcatcg
     6601 atggtatccg taccacgcgg gtacgcggca gatcgacgtc tgcaccctgg ccataggtca
     6661 gcagatcgat gtcaacgccg ttctccgcca gcaccagcgt ccggtaatag acactgaacg
     6721 gcgtgccgcg cggtgtgaag aacggctgcg gagcgatcac cagcgctttc atggcgcgat
     6781 ctgctttgcc ggagtgcgca tccgcacctg catccgcacc tggctgatgg cgaagcgcaa
     6841 cgggttggtc aaacgaccga agcatctggg ggttccgctc agtgcgcgca gccgctgcag
     6901 cagctcctcg acaggctgat cgcttcgatg ccagttctcc agcacgaagc catagctgcc
     6961 cacgcgggcc tcctgaccgg gaaatggaaa tggatcggta cccggcagca ctgcgaaccc
     7021 ctgttgctct gcgcgctgga acaacgttgg acgcggagca ttcgcaagcc gtccgccgtt
     7081 gtcgccgagt gccagcgatt gcggcgcgaa caatgtcagt tgccgccgca ccagttcacc
     7141 gcggcgcagc caccatttgc caaatcccca cggaatgacg ggaaacgcgc cagaagctgc
     7201 cacgcgctcg agcgtcggac gcatggcaag gccgtcgggc acgatctc
//
LOCUS       DATMRM010000115         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_987788, whole genome shotgun sequence.
ACCESSION   DATMRM010000115 DATMRM010000000
VERSION     DATMRM010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987788"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1360)
                     /gene="glgX"
                     /locus_tag="VNZ06_03370"
     CDS             complement(<1..1360)
                     /gene="glgX"
                     /locus_tag="VNZ06_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HWW19822.1"
                     /translation="MSTTTTLPRRPEIGLAHPLGATCDGEGTNFAVYAGNAEEIELCL
                     FDATGRHELHRYLLSECTDGVWHGYLPGVLEGQLYGYRAHGPYEPGQGHRYNVNKLLL
                     DPYARKLAGSLRWSDALFGYRLHSARADLSFDRRDSAFAMPKAVVSVDRFDWGDDRPP
                     RTPWRYTSIYELHVKGFTKRLSLVRERDRGSFAALGSPAAIDYLKQLNITAVELLPIH
                     AFVDDRMLLDRGLCNYWGYNTLAFFAPEPRFLSDGGLNQVKTAIRNLHAAGIEVILDV
                     VFNHTAEGSELGPTLSLRGLDNAAYYRLLGDNPRHHINDTGCGNTVNFSHPRVIQLAM
                     DSLRYWVQHYHVDGFRFDLGVTLGREPHGFDRSSGFFDALMQDPILASTKLISEPWDT
                     GPGGYQLGNHPPGMGEWNGAFRDDLRKFWRGDPGMRSTLAARLQGSADLFDHDTRRPW
                     ESI"
     gene            complement(1382..>2946)
                     /gene="glgB"
                     /locus_tag="VNZ06_03375"
     CDS             complement(1382..>2946)
                     /gene="glgB"
                     /locus_tag="VNZ06_03375"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HWW19823.1"
                     /translation="PPATASVVSHPQPYVWHDLAWLERRSGKDRYAHAPMSIYEMHAA
                     SWRYAQGEARSLSWDELSERLIPYVAELGFTHIEFLPLMSHPFGGSWGYQPLGLFAPQ
                     ATLGEPAALARFIDRCHSANIGVILDWVPAHFPTDPHGLAHFDGTPLYEHTDPREGFH
                     QDWNTLIYNLGRREVHSFLIASALHWLERYHVDGLRVDAVASMLYRDYSRRQGEWIPN
                     KYGGRENLEAIEFLRDLNDVVAHRCPGALTIAEESTAWPGVTRATQAGGLGFPYKWNM
                     GWMHDTLRYLSLDPIHRSYAHDALTFGLVYAFSERFVLPLSHDEVVHGKRSLIGRMPG
                     DQWQRFANLRAYYGFMWAHPGKKLLFMGCEFAQEHEWNHDAQLDWNALADPYHRGVQR
                     LIGDLNRAYVSEPALHALDCDSAGFHWIIGDDRANSVYVFYRSGFAGTAPVIALCNFT
                     PVPRTRYRIGAPRKGRWRELINTDAAVYGGSNLGNGGSVIATDGRSHGQPFSIELTLP
                     PLATLLLRFDES"
BASE COUNT          568 a          924 c          936 g          518 t
ORIGIN      
        1 tgatcgattc ccagggtcgg cgggtgtcat gatcgaacag gtcggcggag ccctgcagac
       61 gcgccgccag cgtgctgcgc atgcccggat cgccgcgcca gaatttgcgc aggtcatccc
      121 ggaaggcgcc attccactcc cccataccgg gcggatgatt gcccagttga tagcctcccg
      181 gcccggtgtc ccacggctcg gagatcaatt tggtgctcgc cagaatggga tcctgcatca
      241 gtgcatcgaa gaatccggag ctgcggtcga atccatgcgg ttcgcgtccc agcgtgaccc
      301 ccagatcgaa gcgaaatcca tccacgtgat agtgctgtac ccagtaacgc agcgaatcca
      361 tcgcgagctg gatcacgcgc gggtgagaaa aattaaccgt attgccgcag ccggtatcgt
      421 tgatgtggtg gcgcgggttg tcgccgagca gacggtagta ggccgcgttg tccaggccac
      481 gcaggctgag cgtcggccca agctcgctgc cttccgcggt gtgattgaac accacatcga
      541 ggatcacctc gatgccggcg gcgtgcaggt tcctgatcgc ggtcttcacc tgattcaggc
      601 cgccgtccga cagaaagcgc ggctccgggg cgaagaatgc cagcgtgttg tagccccaat
      661 agttgcacag gccgcgatcg agcagcattc gatcgtccac gaaggcatgg atcggcagca
      721 gctccaccgc ggtgatgttg agctgcttca ggtagtcgat cgccgccggg ctgcccagtg
      781 ccgcaaagct gccgcggtcg cgctcccgca cgaggctcag ccgtttggtg aatcccttga
      841 cgtgcagctc ataaatactg gtatagcgcc acggtgtgcg gggaggacga tcatcgcccc
      901 agtcgaaacg atccacgctc acgaccgcct tcggcatggc gaatgcgctg tcgcgacgat
      961 cgaatgacag atcagcgcgc gccgagtgca ggcggtaacc aaacagcgca tcgctccagc
     1021 gcagcgagcc ggcgagcttg cgagcgtagg gatccagcag cagcttgttg acgttgtagc
     1081 gatgcccctg ccccggctcg tacgggccgt gtgcgcggta gccgtagagt tgtccctcga
     1141 gcacacccgg cagatagccg tgccagacac cgtcggtgca ctcgctcagc aaatagcggt
     1201 gcagctcatg tcggccggtc gcatcgaaca gacacagctc gatctcctcg gcgttgccgg
     1261 catagaccgc aaagttggtc ccttcgccgt cgcaggtggc ccccagcgga tgagccaacc
     1321 cgatttcagg gcgtcgtggc agcgttgtgg tcgtagacac atggattcct ttctggccca
     1381 tctatgattc atcgaaacgc agcagcagtg tcgccagagg cggcagcgtc agttcaatcg
     1441 agaatggctg accgtgactg cggccgtccg tggcgatcac cgatccgccg ttgcccaggt
     1501 tggagccgcc gtagacggcc gcatcggtat tgatcagttc ccgccagcgt cccttgcgcg
     1561 gtgcgccgat gcggtatcgg gtgcgcggca ccggcgtgaa attgcagagc gcgatgaccg
     1621 gagcggtgcc ggcaaacccc gagcggtaga acacatagac gctgttggcg cgatcgtcgc
     1681 cgatgatcca gtgaaatccg gcgctgtcgc agtccagtgc atgcagcgca ggctcgctca
     1741 cataggcgcg gttcagatcg ccgatcaggc gctgcacgcc gcgatgatag ggatccgcga
     1801 gcgcattcca gtcgagctgc gcgtcatgat tccattcatg ctcctgggca aattcgcagc
     1861 ccatgaagag cagcttcttg cctgggtgcg cccacatgaa gccgtagtag gcacgaagat
     1921 tggcgaagcg ctgccattga tcgcccggca tgcgcccgat cagcgaacgc ttgccgtgta
     1981 ccacctcatc gtgcgacagc ggcagcacga aacgctcgga gaaggcatag accaggccga
     2041 aggtgagcgc atcgtgcgcg taggaacgat ggatcggatc cagtgagaga tagcgcagcg
     2101 tgtcgtgcat ccagcccatg ttccacttgt aaggaaagcc caggcccccg gcctgcgtgg
     2161 ctcgggtcac gcccggccag gcggtggact cctcggcgat cgtcaatgcc cccggacagc
     2221 ggtgcgcgac cacatcgttc agatcgcgca ggaactcgat ggcttccaga ttttcgcgac
     2281 cgccgtattt gtttgggatc cactcgccct gccggcgtga gtagtcgcga tagagcatcg
     2341 aggccacagc gtcgacccgc aggccgtcca cgtggtagcg ctcgagccag tgcaaagcac
     2401 tcgctatcag gaacgaatgc acttcgcgcc gacccaggtt gtagatcagc gtattccagt
     2461 cctgatgaaa tccctcgcgt ggatcggtgt gctcatacaa cggcgtgccg tcaaagtgcg
     2521 ccaggccgtg cggatcggtc ggaaagtgcg ccggcaccca atccagaatg acgccgatgt
     2581 tggcgctgtg gcagcggtcg atgaagcgcg ccaaggccgc gggctcgccc agtgtcgcct
     2641 gaggtgcgaa cagacccagc ggctggtagc cccaggaacc gccgaacgga tgactcatca
     2701 gcggcaggaa ctcgatgtgg gtaaagccca gctccgccac atacggaatc agtcgctcgg
     2761 acagctcgtc ccaggaaagg cttcgcgcct cgccttgcgc atatcgccag gaggccgcgt
     2821 gcatctcata gatcgacatc ggcgcgtgtg cataccgatc cttgccggat cgccgctcca
     2881 gccacgccag gtcgtgccac acgtaaggtt gcggatgact gaccacggaa gcggtcgccg
     2941 ggggtg
//
LOCUS       DATMRM010000116         9298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_376883, whole genome shotgun sequence.
ACCESSION   DATMRM010000116 DATMRM010000000
VERSION     DATMRM010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376883"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VNZ06_03380"
     CDS             complement(<1..295)
                     /locus_tag="VNZ06_03380"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HWW19824.1"
                     /translation="MSSPSTEVVYGLHTVRMLLTRQPQRVSRVFLQAGRNDPRAAQIE
                     ALARSAQRPVERLDGRRFESRLGNVVHQGVMAQVTPLRPWQEEDLFEALVKASE"
     gene            complement(276..2165)
                     /gene="rnr"
                     /locus_tag="VNZ06_03385"
     CDS             complement(276..2165)
                     /gene="rnr"
                     /locus_tag="VNZ06_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HWW19825.1"
                     /translation="MSANRAGYGFVRTDSLSESVFLPPTEMRGLVHGDRVRVEVHSDT
                     QGRYVGRLLDVVERGVQAFLGILEAAGRALIVRSADQRLGLSCRVTDNATSARAGDWV
                     IGRVLKYPNDGVSGEARVEQRLDPDKPVQMATEAAIARYGLPIEFSAEALREAAQCGR
                     HVDPAEAAQRTDLRALALVTIDGDDAKDFDDAVYAERLTGGAFRLMVAIADVSHYVRP
                     GSALDREALERGTSVYFPTRVLPMLPTALSNHLCSLEPQVDRLCMVADMRVSKRGALE
                     ESRFYPAVMRSAARLTYTQAHAALFQGESSARERLGALLDAMMPLVDVYRALTAARRR
                     RGALDFDAPEAEFVIENGERISAIEFRSRNDAHRLIEECMVLANVAVAREMRERHQPA
                     LLRVHAAPDAKKLEQLASTLRVLGIELQLPDEVQPRDLAAIAHQVRDPQSRPFVESLV
                     VRSMAQALYSDEHIGHFGLALKDYAHFTSPIRRYPDLMIHRALRARLGLLDASELPAG
                     AALGARGIELSRLERRADEADRYVGTFLKCVYLRDRVGQTFDGLITTVMEFGCFVQLL
                     GLGVDGLLPLSALRDDDYQMARDGAQWIGAHNKRRLAPGVRLRVVVAGVKPVEGQIDL
                     ELALD"
     gene            2358..2442
                     /locus_tag="VNZ06_03390"
     tRNA            2358..2442
                     /locus_tag="VNZ06_03390"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2392..2394,aa:Leu,seq:cag)
     gene            complement(2587..3078)
                     /locus_tag="VNZ06_03395"
     CDS             complement(2587..3078)
                     /locus_tag="VNZ06_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWW19826.1"
                     /translation="MKKTTVIALDRIDTRMLALIQHNARIPIIELAKSVSLSPSACAR
                     RLRLLEDGGVIRGYGARIDAAAVGLPMVAYVLVSMSRISDDALASFEAAIAKAPEVME
                     ACLMTGKEDYLLKVVVPDLAAYEQFVKQRLTRIPGVSHVETSFMLRQVTARAALPLPL
                     PAS"
     gene            3196..4422
                     /locus_tag="VNZ06_03400"
     CDS             3196..4422
                     /locus_tag="VNZ06_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWW19827.1"
                     /translation="MPTRQSRLHIPATPARPGTPPDFSYLRVSPAGAMPMPAINASLD
                     STLPLAQGLVRVLDDAHDAVGPWRPKVDAQALKTGLRNMLLTRVYDKRMQRTHRQGRI
                     SFYMQSTGEEAVSVAAAMALQPQDMLFPSYRNQGLFITRELPLTDMMCQLLSNRHDMC
                     KGRQLPVMYHSGPKRLFSISGNLCTQFPQAVGWAMASAIKGESHLASVWIGEGSSAEA
                     DFHHGMVFASVYRAPCILNIVNNQWAISTFQGFAGGEQSSFAARGPGYGIPGIRVDGN
                     DFLAVYAVTDWAARRARANHGPTLVELVTYRAEAHSTSDDPSRYRPRDDYQHWPLGDP
                     IERLKQYLMKLGQWSEADHANLIQQLETQVDASWVEAQAQSEPPLDRNLMFEDVFKEL
                     PEHLRKQRDALNAERG"
     gene            4425..5441
                     /locus_tag="VNZ06_03405"
     CDS             4425..5441
                     /locus_tag="VNZ06_03405"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWW19828.1"
                     /translation="MAQMNMIQALHSAMEVMLERDPSVVLMGEDVGYFGGVFRATEGL
                     QRKFGEHRVLDTPIAEGGILGTAIGMGVNGLRPVAEIQFSDYIYPAFDQIVSELARLR
                     YRSGGSFSSPVTVRTPCGGGIRGGQTHSQSPEGIFTHVCGIKVVMPSNPYDAKGLLIA
                     SIEDDDPVIFFEPKRLYNGPFDGTPDKAAVAWKGHARAEVPDEHYVVPIGQAEVVRQG
                     SAMTIVTYGTMVHVCEAAVKDTGVDAEIIDVRSMIPLDEDRICSSVCRTGRCLVVHEA
                     TGFSGFGAELSSTIQEHCFWNLEAPIERVTGWDTPYPSAFEWDYFPGPKRVGAAIQRV
                     MNPS"
     gene            5438..6643
                     /locus_tag="VNZ06_03410"
     CDS             5438..6643
                     /locus_tag="VNZ06_03410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWW19829.1"
                     /translation="MSRYAFQLPDLGEGTVCAEIVSWLVKPGDQVSEGQPIAEMSTEK
                     AVVELPSPVTGKVISLGGAPGDEIAVGAELITFEQGAAAPASAPPSAASARPAVSNGH
                     DRAQGETLRVRTSPATRRRAREAGIDLTQVTGTGPRGRIQPEDLTQALAAKLPARAPI
                     APADIHEGPATEEVKVIGVRRVIAQRMSAAKRSIPHFSYVEEIDVTELEALRVHLNRD
                     PRFEQSRLTLLPFIVKAVARALERFPQCNALYDESRDTLVRHRAMHAGIATQTRDGLK
                     VPVVRNAQALSLQQLSAEIRRVSEAARSNRAKREELSGSTITLTSLGKLGGIASTPVI
                     NMPEMAIIGVNRMSERAVVRHGGITVRQMMNLSSSFDHRFVDGFDAAAMIQAVKELLE
                     HPATIFIET"
     gene            complement(6648..8114)
                     /locus_tag="VNZ06_03415"
     CDS             complement(6648..8114)
                     /locus_tag="VNZ06_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HWW19830.1"
                     /translation="MEPVASRLPSEPVASARLPAQRIDAHGREHIDQRAVWALALPLM
                     ANSAVQIVLNLTDVWFVGRLSTQALAAVGSVQWLVLVVVLVVGSVSMGVQTWVAQAQG
                     ARHYRRASQAVWTALWGVLISSPIFIAIGAARHLILAPAGIDPDISVLASEFWFPRVG
                     GAFLGAAVWCTFGFFNGVGRPRTTLWVTCAIALTNAILNQIFIFRLGLGVAGSGWAST
                     VAQAVGLLLALAVFVGPQYRTRYRSNLTWRPSLRRIAEQTRLGFPMGLPSAGDVLGFA
                     IFQMMQVRLGVIGGAATQVVMLMTSIAYLPGWGIASAGTTLVGQSIGAGHPGWAAHVG
                     SRVILLAGLFMGGIGIVLAACGPWILPLFAQSHDAQAAAMVNLGTRLLWIAAVYQFFD
                     GINMGSSMALRGAGDVMVPAAMVLPLSMLLLVPLAHVLTFAPGEGWFHFLPQLGWGAT
                     GGWIAVVVYLVILASALFLRWRSGAWRKIKLRARKLRA"
     gene            8178..>9298
                     /locus_tag="VNZ06_03420"
     CDS             8178..>9298
                     /locus_tag="VNZ06_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HWW19831.1"
                     /translation="MTSDLQTLAEASGFQRTGRSDEVARLCAAYGSRWPDAVRVLEFG
                     RSSDGRPMLALLVTRCGHLDAPGLRAASIPLLFVQAGIHPGESDGKDAGFIALRELLQ
                     GEVLAAALREIAILFVPAFNVDGHERMGRWNRPNQNGPEETGWRVTARNLNLNRDYCK
                     ADTPEMQGLLRLISEWDPLVCADLHVTDGADFQPDISLQAEPINQGDPRLYAAALQLR
                     DQVIARLAAQGAKPLSFYPDFHVTDDPSSGFVLTVYSPRFSTGYFPQRNRFTLLVETH
                     SWKDYRTRVRVTRNTIINTVEALCEHGTAWLQQVRAADLAAAQIAGTEQVLDFRSSWR
                     EPGQAGSNGKSPTDSDTASDVRMIDFPGYAYTRTRSQISG"
BASE COUNT         1830 a         3058 c         2808 g         1602 t
ORIGIN      
        1 gctcgctcgc cttgaccaac gcctcgaaca ggtcctcttc ctgccacgga cgcaacggcg
       61 tgacttgcgc catcacgccc tgatgcacga cattgcccag gcgggactcg aatcgtctgc
      121 catccaggcg ctcgaccggg cgttgcgcac tccgcgccag cgcctcgatc tgggcggcgc
      181 gcgggtcatt gcgacccgcc tgcagaaaca cgcgcgagac gcgctgcggt tgtcgggtca
      241 gcagcatccg cacggtgtgc agaccgtaga caacttcagt cgagggcgag ctcaagatcg
      301 atctgccctt ctactggttt gacgccggcg acgaccacgc gcagccgcac tcccggggcg
      361 agtcgccgct tgttgtgggc gccgatccac tgtgccccat cgcgtgccat ctgatagtca
      421 tcatcgcgca gtgccgacaa cggcagcagg ccatcgacgc cgagccccaa caactgcacg
      481 aagcagccga attccatcac ggtcgtaatc agcccgtcaa aggtctgacc gacccgatcg
      541 cgcagataca cgcacttgag aaacgtgccg acatagcgat ccgcttcatc cgcgcgacgc
      601 tccagccggg ataactcgat gcccctggcg ccgagcgcag caccagcagg caattcgctc
      661 gcatccagca agccgagtcg cgcacgcaat gcacgatgaa tcatcagatc cgggtagcga
      721 cggatgggag aggtgaagtg agcgtagtcc ttcaacgcca gaccgaagtg cccgatgtgc
      781 tcgtcgctgt agagtgcctg cgccatggaa cgcaccacca gcgattcgac gaacggccgg
      841 ctctgtggat ccctgacctg atgcgcaatc gcagccagat cccgcggctg gacctcatcg
      901 ggcaactgca gttcgatccc cagaacccgc aaggtcgagg cgagctgttc gagtttcttg
      961 gcgtccggtg ccgcgtgcac gcgcaacagc gccggctggt gccgttcgcg catttcgcgc
     1021 gctaccgcca cattggcaag caccatgcat tcctcgatca gccgatgcgc atcattgcgt
     1081 gaacggaact cgatcgcgga aatgcgctcg ccgttctcga tcacgaattc agcctcgggc
     1141 gcatcgaaat cgagtgcccc gcgccggcga cgggccgccg tcagcgcccg atagacatcc
     1201 accagtggca tcatcgcatc cagcaacgcg ccgaggcgct cgcgcgccga agactcgccc
     1261 tgaaacagcg ccgcatgcgc ctgcgtatag gtcagacgcg cggccgagcg catcaccgcc
     1321 ggatagaacc tgctctcttc aagcgcgccg cgtttcgaaa cgcgcatgtc cgccaccatg
     1381 caaagccggt ccacctgcgg ctcgagtgag cacagatgat tcgacagcgc cgtcggcaac
     1441 atcggcagca cccgcgtcgg aaagtaaacc gaggtgccgc gctcgagcgc ttcgcggtcg
     1501 agcgcactgc ccggccgcac gtagtgactg acgtcagcga tcgcgaccat cagcctgaat
     1561 gcaccgccgg tcaaccgctc ggcgtacacc gcatcgtcga aatccttggc gtcgtcgcca
     1621 tcgatggtga ccagcgccag cgcgcgcaga tccgttcgct gcgcagcttc ggccgggtcg
     1681 acatgcctac cgcattgcgc cgcctcgcgc agcgcctcgg cagagaactc gatcggcaag
     1741 ccgtagcgcg cgatcgccgc ttcggtcgcc atctgcaccg gtttatccgg atcgagtcgc
     1801 tgttcgaccc gcgcctcgcc gctgacgcca tcgttcgggt acttgagtac tcgcccgatc
     1861 acccagtcgc cggctcgcgc gctcgtcgcg ttgtcggtca cacgacagga caaaccgagc
     1921 cgctgatcgg ccgaacgcac gatcaaggcc cgacccgcag cctccaggat tcccaggaag
     1981 gcctgcacgc cgcgctcgac cacgtccagc agtcgcccga cgtaacggcc ttgcgtgtcg
     2041 ctgtgcacct caacgcgcac ccgatcgcca tgcaccagcc cgcgcatttc cgtgggcggc
     2101 agaaacacgg actcgctcag cgagtcggta cgaacgaagc catatccggc acgattcgcg
     2161 ctcaacacac cttcggcctg aatcacggcg ccgatcgacg gcgcgcggcg agcccttgct
     2221 gtgctcggat gcccaccgcg cggcctgatc tctttgtgac tgcgatgacg cgccaccgat
     2281 ttggcacgcg tctgttttcc tctacgagtc aattgacagt ccccgacccc ttccgtacat
     2341 tgcgccgccc acccgatgcc caggtggcgg aattggtaga cgcactagtt tcaggtacta
     2401 gcggggcgac ccgtggaggt tcgagtcctc tcctgggcac catgcgaaga ataatatcct
     2461 ttaaaatcat aaaattaata agtatttctg tagttatttc tagggtaagt cgagtgaatt
     2521 gccagcaggc ttgggtggca ggacatgcga ccgctgtgcg cccctggtag gcgcagtgta
     2581 tcagcgtcag ctcgcgggaa gcggtaacgg tagcgcggcg cgcgccgtca cctgccgcag
     2641 catgaaactg gtctccacgt gcgatacgcc tggaatgcgg gtcaggcgct gcttgacgaa
     2701 ctgctcgtag gctgccaggt ccggcaccac gaccttcagc agatagtctt ccttgccggt
     2761 catcagacag gcctccatca cctccggggc cttggcgatc gccgcttcga aactcgccag
     2821 tgcgtcgtca ctgatgcggc tcatgctcac cagtacatag gccaccatcg gcaagcccac
     2881 cgcggcggcg tcgatgcgtg cgccgtagcc acggatcacc ccaccgtcct ccagcagtcg
     2941 caaacgacgg gcgcaagccg acggcgagag cgagacactc ttggcgagct cgatgatcgg
     3001 aatgcgcgcg ttgtgctgaa ttagcgcaag catgcgcgta tcgatccgat cgagcgctat
     3061 gacagtggtt tttttcatga aatgctttga ttttcgaaaa taagttgaaa gttctaagct
     3121 tttgtggcgc tcctagcaag cctacgtcag tttagccggc taaaccttaa cgtggcaccg
     3181 ctatacatta tggatatgcc cacccgtcag tcgcgtctgc acatcccggc cacgcccgcc
     3241 cgtccaggga ccccacctga cttcagttac ttgcgagtat ccccagctgg cgcgatgccg
     3301 atgcccgcga tcaacgcctc gctcgacagc acgctgccac tggcacaggg gctggttcgg
     3361 gtactcgacg acgcgcacga tgcggtgggt ccgtggcgcc cgaaggtcga cgcacaggcg
     3421 ctcaagaccg gactgcgcaa catgctgctg acccgcgtat acgacaagcg catgcagcgc
     3481 actcaccgcc agggtcgcat ctcgttctat atgcagtcca ccggcgagga agccgtatcg
     3541 gtcgcggccg cgatggcgct gcagccgcag gacatgctgt ttccctccta tcgcaatcag
     3601 ggattgttca tcacacgcga actgccactg accgacatga tgtgccagct gctgtccaat
     3661 cgtcatgaca tgtgtaaggg acgacagcta cccgtcatgt atcactcggg cccgaagcgc
     3721 ctgttttcaa tctccggcaa tctgtgcacg cagtttccgc aggccgttgg ctgggcgatg
     3781 gcctccgcga tcaagggtga gtcccacctc gcctcggtgt ggatcggcga aggcagcagc
     3841 gcggaagcag acttccacca cggcatggtg ttcgcttccg tctatcgcgc gccctgcatc
     3901 ctcaacatcg tcaacaacca gtgggctatc tccactttcc agggttttgc cggcggcgaa
     3961 cagtcctcgt tcgcggctcg cggacccggg tacggcatcc cgggaatccg cgtcgatggc
     4021 aatgactttc tcgcggttta cgcggtcacc gattgggcgg cacgaagggc gcgcgccaat
     4081 cacggaccga cgctggttga actggtcacc tatcgcgctg aagcgcactc gaccagtgac
     4141 gacccgtcgc gctatcgtcc gcgcgatgac tatcagcact ggccgctcgg cgatccgatt
     4201 gaacgcctga agcagtacct gatgaaactg ggccaatggt cggaagccga tcacgccaat
     4261 ctgatccagc aactcgagac tcaggtcgat gcgagctggg tcgaagccca ggcgcagagt
     4321 gaaccgccgc tcgatcgaaa tctgatgttc gaggatgtgt tcaaggaact gcccgaacat
     4381 ctgcgcaagc agcgcgatgc gctcaatgcg gagcgaggct gagcatggca cagatgaaca
     4441 tgattcaggc gctgcactca gcgatggagg tcatgctcga acgtgacccc agcgtcgtat
     4501 tgatgggcga agacgtcggc tatttcggcg gtgtgtttcg cgccaccgaa ggactgcaac
     4561 gcaagttcgg tgagcatcgc gtactggata cccccattgc cgaaggcggc attctgggca
     4621 ccgcgatcgg aatgggcgtc aatggattgc gaccggtcgc cgagattcag ttctcggact
     4681 acatttaccc ggcgttcgat cagatcgtga gcgagctggc gcgcttgcgc taccgcagcg
     4741 gtggcagctt ttcgtcccca gtgaccgtga gaactccctg cggtggcggt atccgcggcg
     4801 ggcagacgca ctcgcagagc ccggagggta tcttcacgca cgtgtgtggc atcaaggtcg
     4861 tgatgccctc caatccgtac gatgccaagg gcctgttgat cgcctcgatc gaggatgatg
     4921 atccggtgat ctttttcgag ccgaagcgcc tgtacaacgg cccgttcgac ggcacgccgg
     4981 acaaagcggc cgttgcctgg aaaggacacg cgcgggccga agtcccggac gagcactatg
     5041 tcgtacccat cggccaagcc gaggtggtgc gccagggaag cgccatgacc atcgtcacct
     5101 acggcaccat ggtgcacgtg tgcgaagctg cggtgaaaga caccggcgtg gatgccgaga
     5161 tcatcgacgt gcgctcgatg attccgctgg atgaggacag gatctgcagt tcggtatgcc
     5221 ggaccggtcg ctgtctggtg gtgcacgaag cgaccggctt ctcgggattt ggcgccgagc
     5281 tgtcatcaac cattcaggag cactgcttct ggaatctcga agccccgatt gagcgtgtga
     5341 ccggttggga tacaccctac ccgagtgcct tcgaatggga ttattttccg ggtcccaagc
     5401 gcgtgggcgc tgccatccag cgagtaatga atccctcatg agccgctatg catttcaact
     5461 gcccgatctg ggtgaaggca cggtctgcgc ggaaatcgtg agctggctgg tgaagcccgg
     5521 cgatcaggtc agcgaaggcc agccgattgc cgagatgtcg accgagaagg ccgtggtcga
     5581 actgccctca ccggtcaccg gcaaggtgat ctcgctcggc ggtgcgcctg gcgatgagat
     5641 cgcggtcggc gctgaactga tcaccttcga acagggcgcg gcagcgcccg ccagcgcgcc
     5701 tcccagcgca gcatcggcac ggcccgcggt ctccaacggg catgacaggg cacagggcga
     5761 gaccctgcga gttcgcacct cgccggcaac gcggcgtcgg gcccgcgaag cgggaattga
     5821 tctcacccag gtcaccggca caggtcctcg cggacgcatt cagcccgagg atctcaccca
     5881 ggcgcttgca gccaagttgc cagctcgcgc accgatcgcg ccggccgaca tacacgaggg
     5941 cccggccacc gaggaagtca aggtcatcgg cgtgcgacgt gtgatcgcgc agcgcatgag
     6001 cgcggccaaa cgcagcatcc cgcacttcag ttacgtcgaa gagattgatg tcactgaact
     6061 ggaagcgctg cgggtacacc tcaatcgtga cccgcgtttc gagcagtcac gactgaccct
     6121 gctgcccttc attgtcaaag ctgtggcgcg cgcactcgag cgtttcccac agtgcaatgc
     6181 tctctacgac gagtcgcgcg acacgctcgt gcgtcatcgg gcgatgcacg ctggcattgc
     6241 cacgcaaacc agagatgggc tcaaggttcc tgtcgttcgc aatgcgcagg ccctgtcgct
     6301 gcagcagctg tcggcggaga ttcgacgcgt ctcggaggca gcgcgcagca atcgcgccaa
     6361 gcgcgaggaa ctgagcggct cgaccattac cctcaccagt ctcggcaaac tcggcggcat
     6421 cgcctcgacc ccggtcatca acatgccgga aatggcgatc attggcgtca atcgaatgag
     6481 tgagcgtgcg gtggttcgcc acggtgggat cacggtgcgc cagatgatga acctctcatc
     6541 ctcgttcgat caccgcttcg tggatggttt cgatgccgca gcgatgattc aggccgtgaa
     6601 ggaactgctg gaacacccgg cgaccatctt cattgaaact tgattcatca cgccctgagt
     6661 tttctcgccc tgagtttgat tttccgccat gccccggacc gccagcgcaa aaacagcgcg
     6721 ctggccagta tcaccagata gaccacgacg gcgatccagc cgccggtcgc accccagccc
     6781 agttgcggca ggaagtgaaa ccaaccttct cccggcgcaa aggtcagcac gtgcgccaac
     6841 ggtaccaaca gcagcatgga cagtggcaat accattgccg cagggaccat gacgtccccg
     6901 gcaccgcgca gcgccatcga gctacccatg ttgataccgt cgaaaaactg gtacaccgcc
     6961 gcgatccaca gtagccgcgt tccgagattc accatggcgg cggcctgcgc atcgtgcgat
     7021 tgcgcgaaca gcggcaaaat ccacggtccg caggcggcca gcacgatgcc gatgccgccc
     7081 atgaaaagac cggccagaag tatcacgcgc gaacccacat gcgcagccca gccgggatgt
     7141 cccgcaccga tcgattgacc cacgagcgta gtgcccgcgg aggcgatgcc ccaccccggc
     7201 aagtaggcaa tggatgtcat cagcatcacg acctgcgtgg ccgcgccacc gatcacaccc
     7261 agccgcacct gcatcatctg gaagatggca aatccaagca catcaccggc cgagggcagg
     7321 cccatcggaa agccgagccg cgtctgctca gcgatgcgtc gcaagctcgg ccgccaggtc
     7381 aggttcgacc gatagcgcgt gcggtactgt gggcccacaa agactgcgag tgccagcagc
     7441 aatcctaccg cctgtgcgac agtcgaggcc caaccagacc cagccacgcc caggcccagc
     7501 cggaagatga atatctgatt cagaatcgcg ttggtaagcg cgatagcgca cgtgacccaa
     7561 agcgtggtgc gtgggcgacc gacaccgttg aagaaaccga acgtgcacca tacggcggcg
     7621 cccaggaagg cgccaccgac gcgcggaaac caaaactccg aagctaacac cgagatgtcg
     7681 ggatcgatgc cggcgggcgc caaaatcaga tggcgcgccg ccccgatggc gataaagatc
     7741 ggagagctga tcagcacgcc ccacagcgcg gtccagaccg cctgagaggc ccttcgatag
     7801 tgacgcgcgc cctgggcctg cgccacccag gtctggactc ccatggacac ggaaccgacg
     7861 accagtacca ccaccagcac cagccattgc acggaaccca ctgcggccag cgcctgcgtc
     7921 gacagacgtc cgacgaacca tacgtccgtc agattcagca cgatctgaac cgcactgttg
     7981 gccatgagcg gcagcgccag cgcccagacg gcccgctgat cgatgtgctc gcgcccatgc
     8041 gcatcgatcc gctgcgccgg caagcgcgcg gatgcaacgg gttcgctcgg cagccgtgag
     8101 gctactggtt ccatgagagt gattactcgc cggggtctga tggcgcgcgg cgccgcgtgc
     8161 atggcgtacg atacagcatg acgagcgatt tgcagacatt ggccgaggcc agcggtttcc
     8221 agcgtaccgg ccgcagcgac gaagtcgcgc gactgtgtgc ggcctacggc agccgttggc
     8281 ccgacgcggt tcgcgtactg gagttcggac gctcgagcga cggccggccg atgctcgcgt
     8341 tgctggtgac gcgttgtggg catttggatg caccgggact gcgtgctgcc tcgatcccgc
     8401 tgctcttcgt acaggcgggc atccatccgg gtgagagcga tggcaaggat gccgggttca
     8461 tcgcactgcg cgagttgctt cagggtgagg tgctcgccgc agcgctgcgc gagattgcga
     8521 ttctgtttgt gccggcgttc aacgtggacg gtcacgagcg catgggacgc tggaaccgac
     8581 cgaatcagaa cggaccagag gagaccggtt ggcgcgtgac ggcgcgcaac ctgaatctca
     8641 atcgcgacta ctgcaaagcc gatacacccg agatgcaggg gctgctgcgc ctgatcagcg
     8701 aatgggatcc gctggtgtgc gccgatctgc acgtcaccga cggcgcggac ttccagccgg
     8761 acatttcgct gcaggcagaa cctatcaatc agggggatcc gaggttgtac gccgcggcgc
     8821 tacagctgcg cgaccaggtc atcgcacgac tggccgcgca gggcgccaaa cccttgagtt
     8881 tctatcctga cttccatgtc acggacgatc cttcctctgg gttcgtgctg accgtgtatt
     8941 caccgcgctt ttccacaggc tatttcccac agcgcaatcg cttcacgctg ctggtggaaa
     9001 cgcattcctg gaaggactac cgcacgcggg tgcgcgtcac acgcaatacg atcatcaaca
     9061 cggtcgaggc gctgtgcgag cacggcaccg cctggctgca gcaggtacgc gctgccgatc
     9121 tggcagcggc gcagatcgcc ggcaccgagc aggtgctcga cttccgctcg agctggcgcg
     9181 aacccggcca ggcaggctcg aacggcaaga gcccgaccga ttcagacacg gcctcagacg
     9241 tgcgcatgat cgacttcccc ggatacgcct acacccgcac ccggtcgcag atctccgg
//
LOCUS       DATMRM010000117         6439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1916287, whole genome shotgun sequence.
ACCESSION   DATMRM010000117 DATMRM010000000
VERSION     DATMRM010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1916287"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1618
                     /locus_tag="VNZ06_03425"
     CDS             <1..1618
                     /locus_tag="VNZ06_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWW19832.1"
                     /translation="INPANLPAGNFTNPPALLQYYNEPFPAGVDRLSSPEYRPDTTLD
                     TVDLAALTLSYDFGFATLTSATSWAHHDNNTAADETAEYLNFPSFPQDLYGQNPRMFI
                     LGLERYNDKPFSQEVRLASQSGGFFEWVGGLFFKHQETIIEENDYYPGYNDYFNACSA
                     QFGASPADFVTPSVCGVGDTAYVPGQNTVIGGVPIIKDQVFTSTFDTKANDYAAFGEL
                     TAHLTSAWSVTAGLRLFRQTVEQSQQSALSLLAAPQFGTPPTSNTTSNSYGRLLGKFN
                     TSYQINSTNLVYATWSQGFRRGGVNALPAVAGGLATPPSIFKVQPDTADNFEIGFKGT
                     FDNRLRYSLALFDVQWHNIQELVQLTPIVLPGVINIGDGYSRGIEFESDAAVTSHLTT
                     HIGYTYDQTKLTSISPLYVFPNVDGTPPSSGSVLPGTPKTSLIGGFEYGHVPLGIGDL
                     RFAINAHYRSSTRSALSSTIPDVPGFTMLDTRLAYALPHLVITAYVDNLTNNLGITSF
                     QDPAVFGNRYLAIVSQPRTYGLTLNYSFKQ"
     gene            complement(1608..3047)
                     /gene="hydA"
                     /locus_tag="VNZ06_03430"
     CDS             complement(1608..3047)
                     /gene="hydA"
                     /locus_tag="VNZ06_03430"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009941.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HWW19833.1"
                     /translation="MRATAEFDLVIRGGTLATAADVFNADIGVRDGKIVAIGARLAGG
                     SDEIDARDKYVLPGGVDAHCHLDQPSSDGSVCADDFRSGTIAAACGGTTTVIPFAAQA
                     KGQSVRAAVEDYHRRADNKAVIDYAFHLIVSDPGEQVLREELPALIRAGYSSFKIYMT
                     YEDLKLSDRQALEVLAVARRERALVMVHAENSDVIGWLTDQLEAAGQTEPRYHALSRP
                     VVAEREATHRAISLSQIVGVPILIVHVSARDAAEQIQWAQRRGLPIYAETCPQYLFLT
                     EGDLARPGFEGAKCLCSPPPRDRHHQEALWLGLRTGVFQVFSSDHAPTRFNDPKGKML
                     HGSQASFRYVPNGVPGIETRLPLLFSAGVVGGRLDLTRFVALTASNPARIYGLYPRKG
                     TLAIGADADIVVWDPTQTRVIRNEHLHHAVDFTPYEGMRATGWPVITISRGDVVYRDN
                     EVLGRPGRGVFLPCGTPEPASRFADQITA"
     gene            complement(3058..3786)
                     /locus_tag="VNZ06_03435"
     CDS             complement(3058..3786)
                     /locus_tag="VNZ06_03435"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19834.1"
                     /translation="MSQRKLLGMLTPSSNTVLEPTCAAMLAGVPNVTAHFARFRVTQI
                     ALSATALRQFDEQPMLQAAELLADARVNAICWNGTSAGWLGLHADRTLCKAIGQRTGI
                     GATTSLLALEEIFRSSGVKRYALVTPYLDEVQRAIIATFRGEDFECIAERHLELRDNF
                     SFSEVAGPTVADMVRSVAVSRPDAIAIVCTNLRAAALVEELERQTGVPIYDTAATAVW
                     GALRVAQIDPKQVLGWGRLFRELA"
     gene            3807..5147
                     /locus_tag="VNZ06_03440"
     CDS             3807..5147
                     /locus_tag="VNZ06_03440"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW19835.1"
                     /translation="MSTVRWRIFLLILLVVSINYVDRASVSVAMPIIASQYALTPRMQ
                     GVLFSAFFWSYTLMQVPGGWLADRLRPRIVIAAATILWGICQVATAAVTGWSGLLLAR
                     IGLGLAEGPIYPASGKLNAIWLPASERGRGAVIIDGGAPLGTALGSLIIATLIGAFNS
                     WRLAFLIAGLGTVAAGVLAHWYIRNTPREHPAVSADELEYIEGAQSREDAQSATTAGA
                     SVPMSKFFRSRSFWGMVLGWLGFDLVFYGLVSWGPSYLAEARHLGLGSVGGSLFIIFG
                     AGFLGEICGGFLADRWKARGASPNLVMRTLLGTSGALTTLSTFALAFVSTSGAAVALL
                     ASILFFLRWAGLYWSLPATLGNRSQAGVLGGIMNAAGNLGGVLVPIVVGAIVQSTGSY
                     FAALLFFAACGLLYTISSLLIDYRVVPPPSSKYSPCIRSHRTAGHRGEDHVRQV"
     gene            5131..>6439
                     /locus_tag="VNZ06_03445"
     CDS             5131..>6439
                     /locus_tag="VNZ06_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HWW19836.1"
                     /translation="MSAKSDYSRRQVIGAGIAIGAGLAGGLLLPRTAVSDTQPVADTS
                     AGKVRGISQEGVNVFKGIAYGASTAGANRFMPPQPPAPWTGVRNAFDYGLSTPQSDPT
                     VTRGPSPTAPFIGELSDRPEGEDCLVLNIWTRGLKDHGKRPVMFWIHGGGFQSGSGSS
                     QGYDGTHLCKRGDVVVVTINHRLNIMGFAFLAEIGGAQFANTGNVGMLDIVQALKWVR
                     ANIENFGGDPHRVMIFGESGGGRKVGTLLAMPDARGLFHRAVIESGPSIKVVERTDAT
                     FAAQAVLDELQISRDHLRELQNVPLNRLMPAFFAASRKHRFNHTTTGFAPVVDGKVLP
                     SHLFHPTASAVMPEVPVIIGTNRTEMALQLVGDAGAYALDEAGLQARVREAFADKAPS
                     LIDTYRRSAPAASPSELFFLMVSDRNYCAPLMKIAERRSALGKA"
BASE COUNT         1173 a         2119 c         1897 g         1250 t
ORIGIN      
        1 aatcaatcca gcgaatctgc cggcgggcaa tttcaccaac ccgcccgccc tgctgcagta
       61 ctacaacgag ccgttcccgg ccggtgtcga tcggctctcc agccctgaat accgacccga
      121 cacgaccctc gacacggtgg atctggcggc cctgacgctc agttacgact tcggcttcgc
      181 cacgctgacc tccgccacgt cgtgggcaca ccatgacaac aacacggccg ctgatgaaac
      241 cgctgaatac ctgaatttcc catcattccc gcaggatctg tacggtcaga atccgcgcat
      301 gttcatcctc ggactcgagc gctacaacga caaaccgttc tcgcaggagg ttcgcctggc
      361 ctcccaatca ggcggcttct tcgagtgggt cggcggtctg ttcttcaagc accaggagac
      421 catcatcgag gagaacgact actacccggg ctacaacgac tacttcaatg cctgctctgc
      481 acagttcggg gcaagcccgg ctgactttgt gacaccgtcc gtgtgcggtg tcggcgacac
      541 ggcctacgta cccggtcaga acaccgtcat cggcggtgtt ccgatcatca aggatcaggt
      601 gttcacgtcg accttcgata ccaaggccaa cgactacgcg gcttttggcg aactgacggc
      661 ccatctgacc tccgcgtgga gtgtgaccgc aggcctgcgg ctcttccgtc aaaccgtcga
      721 gcagtctcag cagagcgccc tgtcactgct cgccgcgccg cagttcggga cgcccccgac
      781 cagcaacacg accagcaact cgtatggccg cctgctcgga aaattcaata cctcctatca
      841 gatcaattcg accaatctgg tctacgctac ttggtcacaa ggctttcgcc gcggcggcgt
      901 caacgcattg ccagccgtgg ccggcggcct ggcgactccg ccgtcgatct tcaaggtcca
      961 acctgacacg gccgacaact ttgaaatcgg cttcaaagga acgttcgaca atcggctgcg
     1021 ctattcgttg gcgctgttcg acgtgcagtg gcataacatc caggagctgg tacagctcac
     1081 tccgatcgtg ctgccgggcg tcatcaacat cggcgatggc tatagccgtg gcattgaatt
     1141 cgaatccgat gccgccgtca cctcgcacct gacgacgcat atcggttaca cctatgacca
     1201 gacgaagctc acctcgatca gtccgctgta cgtgttcccg aatgttgacg gtacaccgcc
     1261 ctcctcgggc agcgtgttgc ccggcacccc gaagaccagt ctcattggtg ggttcgagta
     1321 cggacacgtc ccgctgggca tcggcgacct gcgctttgca atcaatgcgc attaccgcag
     1381 ctccacccgc tcagcgctgt cctccaccat tcccgacgta ccgggtttca cgatgctcga
     1441 tacgcgcctg gcctacgcgc tgccgcacct cgtgatcacg gcctacgtcg acaacctgac
     1501 caacaactta gggattacct cgtttcagga tccggccgtg ttcggcaatc gctatctggc
     1561 gatcgtctcg cagccgcgca cctacggtct cacgctcaac tattcgttca agcagtgatc
     1621 tggtcggcga agcgacttgc aggctcgggc gttccgcagg gcagaaagac gccccgtccg
     1681 ggtcttccga gcacctcgtt gtcgcgatag accacatcac cacgggagat cgtgatgact
     1741 ggccagccgg tagccctcat cccctcatac ggcgtgaagt ccacggcatg atgcaaatgc
     1801 tcgttgcgga tgacgcgcgt ctgagtcggg tcccagacga cgatatccgc gtcggcaccg
     1861 atcgccagtg tgcccttgcg cgggtacaga ccatagattc gcgccggatt gctcgcggtc
     1921 aacgcaacga aacgcgtcaa gtccagacgc ccaccgacga ccccggccga aaacaacaag
     1981 ggcaagcgcg tctcgatgcc cggcactcca ttgggaacgt agcgaaacga tgcctgactg
     2041 ccgtgcagca tcttgccctt cggatcgttg aaacgtgtcg gcgcatgatc ggacgagaat
     2101 acctgaaaca caccggtgcg cagtcccagc cacagcgcct cctgatggtg cctgtcccgc
     2161 ggcggcggac tgcacaggca cttcgctccc tcaaaccctg gtcgggccag atcgccctcg
     2221 gtcaaaaaca ggtactgcgg acaggtctcg gcatagatcg gcagcccgcg ccgctgcgcc
     2281 cattggatct gctcggcggc atcgcgcgcg gagacgtgca cgatcaggat cggtacgcct
     2341 acgatctgac tcagactgat ggcccgatgg gtcgcctcgc gctccgcaac caccggccgc
     2401 gagagcgcgt gatagcgagg ctcggtctgg cctgccgcct ccagctggtc ggtgagccag
     2461 ccgatgacgt cgctgttctc ggcatgcacc atcacgagcg cccgctcacg tcgggcaact
     2521 gccagtactt ccagagcctg acggtcactg agcttgagat cctcataggt catgtagatc
     2581 ttgaacgagc tgtaaccggc acggatcaag gcgggcaact cctcgcgcag cacctgctcg
     2641 cccggatcgg aaacaatcag gtgaaacgcg tagtcgatga cggccttgtt gtcagcgcgc
     2701 cggtgatagt cctcgacggc tgcgcgtacg ctctgcccct tggcctgtgc cgcaaacgga
     2761 atgaccgtcg tggtgccccc gcatgccgcg gcaatcgtgc cgctgcggaa atcatccgcg
     2821 cacaccgacc cgtcgctgct cggctgatcc agatggcaat gcgcatcgac tccccccggc
     2881 aacacgtact tgtctcgggc atcgatctca tcgctaccgc cggcgagccg cgcgccaatg
     2941 gccacaatct ttccgtcgcg aacgccgatg tcagcgttga agacatcggc ggcggtggcc
     3001 agtgttcctc cgcggatcac cagatcaaac tcggccgtag cgcgcatcac gttctcctca
     3061 ggccagctca cgaaacagcc ggccccaacc cagcacctgc ttgggatcga tctgcgcgac
     3121 gcgcaacgca ccccacaccg cggtggctgc ggtgtcgtag atcggcactc cggtctgtcg
     3181 ctcgagttcc tcgaccagcg ccgcggctcg cagattggta cacacaatgg caatcgcatc
     3241 cggacgcgag accgcgacgc tgcgaaccat atcggccaca gtcggtccgg cgacctcgga
     3301 gaacgaaaag ttgtcgcgca gctctaggtg acgttcggcg atgcactcga agtcctcacc
     3361 gcgaaacgtt gcgatgatgg ctcgctgcac ctcatccagg tacggtgtga ccagcgcgta
     3421 tcgcttcact ccactgctgc ggaatatttc ctccagcgca agcagcgacg tggtagcccc
     3481 gataccggtt cgctgtccaa tcgctttaca tagcgttcga tcggcgtgca ggccgagcca
     3541 tccagccgac gttccgttcc agcaaatcgc attcaccctg gcgtcggcca gcaattccgc
     3601 tgcctgcagc atcggttgtt catcaaactg gcgcagcgcc gtcgcgctca gtgcgatctg
     3661 cgtgacgcga aatcgggcaa agtgggcggt cacattcggc acgccagcca gcatggccgc
     3721 acacgtcggc tccagcaccg tattggatga cggagtgagc atgcccaaca gttttctctg
     3781 gctcatggcg tgacaaagcg tacgccatga gcacggtgcg ttggcgaatc tttctgctca
     3841 tcttacttgt cgtttcgatc aactatgtgg atcgcgcatc agtgtcggtg gcaatgccga
     3901 tcatcgccag ccaatacgca ctcactccgc gcatgcaggg tgtgctgttc agtgcgttct
     3961 tctggagcta cacgctgatg caggtcccgg gcgggtggct cgcggatcgt ctgcgcccga
     4021 gaatcgtcat tgctgccgcg acgatcctct ggggcatttg ccaggtggct accgccgccg
     4081 tgaccggctg gagcggactg ctgctggcgc gcatcggtct gggtctggcc gaaggtccca
     4141 tctatcctgc cagcggtaaa ctgaacgcca tctggctgcc tgcgagcgaa cgcggtcgtg
     4201 gagccgtgat cattgacggc ggcgcgccgc tcggaacggc cttgggaagc ctgatcatcg
     4261 cgaccctgat tggcgcgttc aattcctggc ggctggcgtt tctgattgcc ggactgggca
     4321 cggttgccgc gggagtcctt gcgcactggt atatccgcaa tacgccacgc gagcatccgg
     4381 ccgtcagcgc agacgagctg gaatatatcg agggcgcgca gtcccgcgaa gacgctcagt
     4441 cagcgacgac cgcaggcgca tccgtgccga tgagcaagtt cttccggtca cgatccttct
     4501 ggggcatggt gcttggctgg ctgggctttg atctggtgtt ctatggcctg gtctcctggg
     4561 gcccgagcta tctggccgaa gcgcgccatc tcggcctcgg ctccgtcggt ggctcgctgt
     4621 tcattatctt cggcgcaggg ttcctgggcg agatctgcgg cggctttctc gccgatcgct
     4681 ggaaagcgcg cggagcatcg ccgaatctcg tgatgcgaac actgctgggt acttcgggtg
     4741 cattgaccac gctgtctact ttcgcgctgg cgtttgtcag cacctccggc gcagcggtcg
     4801 ccctgctggc cagcattctg ttcttccttc gatgggcggg tctctactgg agtctaccgg
     4861 cgacgctcgg gaatcgcagt caggcaggcg tgctcggcgg catcatgaat gccgccggta
     4921 acctgggtgg cgttctcgtt ccgatcgtgg tcggcgcgat cgtgcagagc accggtagtt
     4981 acttcgcggc actcctcttc ttcgcggcct gcggcctgtt gtacacgatc agctctctgc
     5041 tgatcgacta tagggtggtg cctcccccat catcaaagta ttctccctgc atacggtccc
     5101 accgaacggc ggggcacagg ggggaagacc atgtccgcca agtctgacta ctcccgtcgc
     5161 caggtgattg gcgcgggcat tgcgatcggc gccgggttag ctggcgggtt gctcctcccg
     5221 cgtaccgctg tttccgacac gcaaccggta gcagacacct ccgctggcaa agtcagggga
     5281 atcagccagg agggagtcaa cgtcttcaaa ggcatcgcct acggcgcctc caccgccgga
     5341 gccaatcgat tcatgccgcc ccagccaccg gcgccatgga caggcgtgcg caatgccttc
     5401 gactacgggc tcagcacccc gcagagcgat cccacggtga cgcgcgggcc ctcacccact
     5461 gcgcctttca tcggtgaatt gagcgatcgg cccgagggcg aggattgtct ggtgctcaat
     5521 atctggacgc gtggcttgaa ggatcacggc aaacggccgg tgatgttctg gatccacggg
     5581 ggcggatttc aatcgggttc cgggtcgtcc cagggctatg acggcaccca cttgtgcaag
     5641 cgcggcgatg tggtcgttgt cacgatcaac catcggctca acatcatggg cttcgccttt
     5701 ctggccgaga ttggcggtgc gcagttcgcg aacaccggca acgtcggcat gctcgatatc
     5761 gtgcaggccc tcaagtgggt gcgggcgaac atcgaaaact tcggcggtga tccgcaccgc
     5821 gtgatgattt ttggcgagtc aggcggcgga cgaaaggtcg gcacgctgct ggcgatgccc
     5881 gatgccaggg ggctgtttca ccgcgccgtc atcgagagcg gtccatcgat caaggtggtc
     5941 gagcgcaccg atgccacgtt tgccgcgcag gccgtactgg acgagttgca gatttcgagg
     6001 gaccacctac gcgagctgca gaacgtgccg ctcaaccgac tcatgccggc tttcttcgcc
     6061 gccagtcgca agcatcgctt caatcacacg accaccggct tcgccccggt ggtcgacggt
     6121 aaagtgctcc cttcacatct gttccacccc accgcctcgg cggtgatgcc ggaggttccg
     6181 gtcatcatcg gcaccaaccg caccgagatg gcgctgcagt tggtcggaga cgcgggcgct
     6241 tatgcactgg atgaggccgg attgcaggcg cgggtacggg aggcgtttgc agacaaagcg
     6301 ccaagcctca tcgataccta ccgcaggtcc gcacccgccg cctctccctc ggaactgttc
     6361 ttcctgatgg tgagcgatcg caactactgc gctccgctca tgaagatcgc ggagcgacgc
     6421 tcagcactgg gcaaggcgc
//
LOCUS       DATMRM010000118         8598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_988633, whole genome shotgun sequence.
ACCESSION   DATMRM010000118 DATMRM010000000
VERSION     DATMRM010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988633"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VNZ06_03450"
     CDS             complement(<1..485)
                     /locus_tag="VNZ06_03450"
                     /inference="COORDINATES: protein motif:HMM:NF036808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmI/RlmK family 23S rRNA methyltransferase"
                     /protein_id="HWW19837.1"
                     /translation="MSVLAEQAATPGASAEIPILRLKRGEDRRLRAGHLWVFSNEVDN
                     DATPLTDIATGSIVRVVSDRDLFLGYAYVNPHALICARIVSRSPVHPPDRSLIEHRLK
                     VALALRERIYSAPYYRLVFGESDGLPGLVLDRYGDTIVGQIATAGMEAMRALVEAAVV
                     NV"
     gene            547..2637
                     /locus_tag="VNZ06_03455"
     CDS             547..2637
                     /locus_tag="VNZ06_03455"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWW19838.1"
                     /translation="MDNRSAQPVNRLKRETSPYLRQHAHNPVDWYPWGAEALERARRE
                     GKPILLSIGYAACHWCHVMAHESFEDAATAALMNELYVNIKVDREERPDLDRLYQLAQ
                     QMLNGRGGGWPLTMFLLHEDQRPFFGGTYFPPEPRHGLPSFSELLRQVATYYHQHLDE
                     LRAPAAQVVGALNDLNPAPMTSHSLSDEPLKTGRARLERSFDGQYGGFGGAPKFPHAP
                     GLARLLRDWHASAQAPTPDLQALYMATLTLTRMAEGGLFDQLGGGFCRYSVDERWEIP
                     HFEKMLYDNAQLLTVYCEAAAATGEVLFADTAVRTAEWMLRELRCAQDGALYSSLDAD
                     SEGHEGKFYVWQQAEVQAALAPAEWEPYAARFGLDGPPNFEGHWHLTVHASREALAER
                     FRLSADELDAALESARQALLAIRATRVRPGLDDKVLVSWNALAVGALAAAARYLNRSD
                     YEQAAGAALTYLRQRHWRDGRLLATSAHGQARLAGYLDDYAFLLDAILELQSVRFRAD
                     EFQWGVQLADVMLLHFDDPRDGGFFFTADDHEALISRPKSFGDEAIPAGNAIAARALL
                     RLGYLLGDTRYLTAAERTLRAAWPALLRYPEAHAAMLQALDDYLHPPQIVVLRGPRAI
                     IEPWSQRLNRAFQPRRWTLAVPAELTGLPEALASKPGSPTGAAYVCRGMTCSAALLSL
                     EALLAELDRETSGA"
     gene            2634..3677
                     /locus_tag="VNZ06_03460"
     CDS             2634..3677
                     /locus_tag="VNZ06_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWW19839.1"
                     /translation="MNARAAIAAALCAALLFGASTPFAKLLAGRVPPIMLAGLLYLGS
                     GLGLSVVRLIRDRGLKPPGLPARQWPWLLGAIACGGILAPILLIVGLARIDASSAALL
                     LNLEAVFTALLAWVVFRENAGRRVLLGMVLIVAGGVTLTGLPGAARQGLAGAMAIVAA
                     CLCWGLDNNLTRKVSGADALFVAAIKGLVAGVTNLLLALRLGDRLPEAGVIGAAMAVG
                     LMGYGISLALYVLALRGLGSARTAAYFSTAPFLGAAIAIALFESGVPMLLWLSAALMG
                     AGVWLHLTEHHEHEHQHDSLTHAHPHVHDAHHRHSHAFPWQGDEPHAHEHEHEPVTHR
                     HAHFPDLHHRHDH"
     gene            complement(3696..4262)
                     /locus_tag="VNZ06_03465"
     CDS             complement(3696..4262)
                     /locus_tag="VNZ06_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HWW19840.1"
                     /translation="MEAFLISLSSVATAEMGDRTQLLALVLAAKFRRPWPIVAGAFCA
                     TLASHVLAGLVGVWAGKLLTPTRLDLMVGVSMIAMALWTLKPDKLDEKQGEVTGASAF
                     VTTLIAFFIAEIGDKTQIATVALAAAYSNLLAVVSGTTCGMMAANIPVIFLGNAFAHR
                     LPLKAIHYAAAALFLGVGAVFLVRSKVF"
     gene            4484..4816
                     /locus_tag="VNZ06_03470"
     CDS             4484..4816
                     /locus_tag="VNZ06_03470"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HWW19841.1"
                     /translation="MGLFDGIIGGLVGGEMASVVNQLIERHGGVQGIIMQMEQQGLGP
                     TVKSWVGTGLNQPISPTQVHQAFGPQILSELAAKTGLNPQELAQKLSQVLPQVVDKLT
                     PNGQVPSH"
     gene            complement(4836..5993)
                     /locus_tag="VNZ06_03475"
     CDS             complement(4836..5993)
                     /locus_tag="VNZ06_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HWW19842.1"
                     /translation="MPRPIDPFEPEQIRRLVREFGSPLLIIDCARVRSQYRKLRGALP
                     GVDLHYALKPLPHAAVVRAIVEAGGWLDLATTGEVQLAQALGAQANRCIHTHPIKRER
                     DINNALEYGVRVFVADNPDEVRKFKPYGERTALLLRVSFRAPGAVCDLSRKFGCDPEG
                     AVELARLAASLGVDVQGFSFHVGSQAPDPLKHVEAIEVCSSLLARARRERLGTLDTLD
                     IGGGFPIEYLKRTAEIRRFCAPIRTALAKLPKRVRVIAEPGRYIAGPSAIGVSTVMGR
                     AQREGHWWYYLDDGLYGSYSGQLYDHSRYPVESLREGVPLLPSVLAGPTCDSIDVIAE
                     NLMLPELKAGDLIVGRAMGAYTWASASEFNFFPKATVVAVNRRPGDAGGVE"
     gene            complement(6010..7038)
                     /locus_tag="VNZ06_03480"
     CDS             complement(6010..7038)
                     /locus_tag="VNZ06_03480"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HWW19843.1"
                     /translation="MVPVVRATHFELDAAVQALRDGELVAFPTETVYGLGAHAGHPAA
                     LRRVFELKQRPTSHPLIVHIDSPRFLTRWAREVPVAAAMLAERFWPGPLTLVLPRAEN
                     VHTLITGGQDTVAVRVPAHPMAQQLLTAFGGGIAAPSANRYGHVSPTRAEHVRDEFGS
                     TVRVVLDGGECKLGLESTIVACLEGTVRLLRPGAVTLSQLQRVVGEVLVDASPYAPRV
                     PGSQRVHYAPTTPLSIVPGDDLERVVANMAESGQRTGVLAMRPPLGTYRLLTWINAGQ
                     RAEAYAHVLYAHLRTLDKVGCARILVQEVPGDERWDAARDRLQRAAAASSEELTDNSD
                     ALALGVLP"
     gene            complement(7069..>8598)
                     /locus_tag="VNZ06_03485"
     CDS             complement(7069..>8598)
                     /locus_tag="VNZ06_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19844.1"
                     /translation="ARVTVRYVTLDDATSSQLRSIRIDRRWYPARACQRDDKVGYWNC
                     TDLPTPANVETPAPASAQPLRVDDKYAAEIAPSLVFVTFDMPYSVSGVTERNYHGVGV
                     IVDAERGLIVTDRNTVPVSIGDVRLTFAGTLEIPGQIVYVHPLHNLTVIHYDPKLIGT
                     TPIRSAKLDSAPLKPGEAVNVIGMDGNGDIKTRSTMIADVDPLQLPLSRSVQFRESNL
                     QVASLVNAPDDYVGVLADNSGKVRGLWASFASDNGRELIQENRGLSSDLLAETLDIVR
                     DNRPLHSLEAEFSEQPLAVARRLGLSDAWVRRIAQTNPSARQVLSVSRLVAGSDAARV
                     LQTGDILLAIDDKPLTQFREVERAVANKDQVTATVWRSDGEKNLQVKTASLSGNDIDR
                     VVEWAGATLQAPHRAISAQRGIPPVGVYVAFFQFGSPASRFGLVPGRRIVEVDGELTP
                     DLDAFLRQVSGRPDRSSLRIKTLSWNGAPEVITLKLDRRYWPAYELNRTAVGWERQSL
                     E"
BASE COUNT         1533 a         2844 c         2735 g         1486 t
ORIGIN      
        1 acgttgacca cggcagcctc taccagagcg cgcatcgcct ccatgcctgc ggtcgcaatc
       61 tgcccaacga tcgtgtcgcc gtagcgatcc agcaccagac ctggcaggcc gtcggactcg
      121 ccgaacacca gtcggtagta cggtgccgaa tagatccgct cgcgcagcgc cagcgccact
      181 ttgaggcggt gctcgatcag cgagcggtcc ggtggatgca ccgggctgcg gctgacgatg
      241 cgggcgcaga tcagcgcgtg cggattgaca taggcgtaac ccaggaacag gtcccggtcg
      301 gacaccacgc gcacgatact gccggtggca atgtccgtca gcggcgtcgc atcgttgtcc
      361 acctcattgg aaaataccca caggtgaccg gcgcgcagcc ggcggtcctc gccccgcttg
      421 aggcgcagta tcggtatttc cgcgctcgcg cccggcgtcg cggcttgttc ggccagaact
      481 gacattgagg tgtgtggcga tccgttatag tggcgcgcca ttttactgaa aattggcttc
      541 cggcccatgg acaaccgctc ggcgcaaccc gtcaatcggc tcaagcgtga gaccagtccg
      601 tatctgcgcc agcatgcgca caacccggtt gattggtacc cgtggggggc cgaggcgctc
      661 gagcgcgccc ggcgcgaagg caaaccgatc ctgctgtcga tcggctacgc cgcctgtcac
      721 tggtgccacg tcatggcgca tgagtcgttc gaggatgcgg cgaccgccgc gttgatgaat
      781 gagctgtacg tcaacatcaa ggtggatcgc gaggagcgac ccgatctgga ccgactctat
      841 cagctcgcgc agcagatgct gaacgggcgc ggtggcggtt ggccgctgac gatgttcctg
      901 ttgcacgagg atcaacgccc attcttcggc gggacctact ttccgcctga accgcgtcac
      961 ggcctaccct ccttcagcga gctgctgcgg caggtagcca cttactacca tcagcacctt
     1021 gatgagctgc gcgcgccggc ggcgcaggtt gtgggcgcgc tcaacgatct gaatccggcg
     1081 ccgatgacat cgcactcgct gagcgacgaa ccactgaaaa ccggccgggc gcggctcgaa
     1141 aggagctttg acggccagta cggaggattc ggcggcgccc cgaagtttcc acacgccccc
     1201 gggctcgccc ggttgttgcg cgactggcat gccagtgcgc aggcgccgac gccggatctg
     1261 caagcgctgt acatggcgac cctgacgctg acccgcatgg ccgagggtgg tctgttcgat
     1321 cagctcgggg gcggcttctg ccgctacagc gtcgatgagc gctgggagat tcctcacttc
     1381 gagaagatgc tgtacgacaa tgcgcagctg ctcaccgtgt attgcgaagc ggcggccgcg
     1441 accggtgaag tcctgttcgc tgatacggcg gtgcgcacgg ccgagtggat gctgcgcgaa
     1501 ctgcgctgcg cgcaggacgg cgccctttat tcgagtctgg atgccgactc cgaaggtcat
     1561 gagggcaagt tctacgtctg gcagcaggcc gaggttcagg cggcactcgc acccgccgag
     1621 tgggagccgt acgcggcgcg cttcggcctg gacggtccgc ccaatttcga gggccactgg
     1681 catttaacag tacacgcgag ccgcgaagca ctggccgagc gctttcggct gagcgccgac
     1741 gaactcgacg cggcgcttga gagcgcacgc caggcgttgc tcgcgatccg tgccacgcgg
     1801 gtgcgccccg ggctggacga caaggtattg gtgagctgga atgcgttggc cgttggcgct
     1861 ctggccgcag cggcccgata tctcaatcgc agcgactacg agcaggcggc cggggctgcc
     1921 ctgacctatc ttcggcagcg gcattggcgc gatggccgcc tgctggcgac gagtgcacac
     1981 gggcaggcgc ggctggccgg ctacctggac gactacgcgt tcctgctcga tgccattctg
     2041 gaactgcaaa gcgtgcgctt tcgggccgat gaatttcagt ggggggtgca gctcgcggac
     2101 gtgatgctgc tgcacttcga tgatccgcgc gacggcggct tcttcttcac cgcggacgac
     2161 cacgaggcgc tgatcagtcg gcctaaatcc ttcggcgacg aggccattcc ggctggcaac
     2221 gccatcgcag cgcgcgcctt gctgcgactg ggatacttgc tcggggacac tcgatatctg
     2281 acggctgcgg aacgcacgct gcgcgccgca tggccggccc tgctcaggta tccggaggct
     2341 cacgccgcga tgctgcaggc gctcgacgac tatctgcacc cgccgcagat cgtcgtcctg
     2401 cgcggcccca gggcgatcat tgaaccctgg agccaacgat tgaaccgcgc attccagccg
     2461 cgacgctgga ctctggcggt tcccgccgaa ctgacgggcc tgccggaggc gctggcgagc
     2521 aagcccgggt cccctactgg agccgcttac gtgtgccgtg gcatgacctg ctccgcggcg
     2581 ctcctgtcgc tcgaggcgtt gctggcggaa ctggatcggg agacatcggg ggcatgaacg
     2641 cgcgcgcggc cattgccgct gcgctctgcg ccgcgctgct gttcggcgcc agcacaccct
     2701 ttgccaaact gctggcggga agggtccccc cgatcatgct ggccggcctg ctctatctgg
     2761 gcagcgggct gggactcagt gtcgtgcggt tgattcggga ccgcggcctc aagccccccg
     2821 gattgcctgc gcgacagtgg ccctggctac tgggtgcgat cgcctgcggc ggaatactcg
     2881 caccgatcct gctgatcgtc ggactggcgc gcatcgacgc gtcgagcgcc gcactgctgc
     2941 tgaatctgga ggcggtcttt accgcgctgt tggcctgggt cgtctttcgc gagaacgctg
     3001 gccggcgtgt gctcctcggc atggtcctga tcgtggccgg cggcgtgacg ctgaccggtt
     3061 tgccgggagc cgcgcgacag gggcttgcgg gagcaatggc cattgtcgcc gcatgcctgt
     3121 gctggggtct ggacaacaat ctgacccgca aggtctcggg cgccgatgcg ctgttcgtcg
     3181 ccgcgatcaa ggggctggtg gcaggtgtca cgaacctgct gctggcgctg cggctcgggg
     3241 accggctgcc cgaagccgga gtcattggcg cggcgatggc tgtcgggctg atgggatatg
     3301 gcatcagcct ggcgctgtac gtgctggcgc tgcgtggact gggctcggca cgcaccgcgg
     3361 cgtacttctc gacggcgcca tttctcggcg ccgcgatcgc gatcgcgctg ttcgaatcag
     3421 gcgtaccgat gctgttgtgg ctgtcggccg ccctgatggg ggccggtgtg tggctgcatc
     3481 tcaccgagca tcatgaacac gagcaccagc acgattcact cacgcatgct cacccgcatg
     3541 tgcacgatgc acatcaccgg cattctcacg cgttcccctg gcaaggcgat gaacctcatg
     3601 cgcatgagca cgaacatgaa ccggtgaccc accggcacgc tcactttccc gatctgcatc
     3661 accgccacga tcactaggcg ggtgggcctg tgtggctaga acactttcga gcgcaccagg
     3721 aacacggcgc cgacacctaa gaacagtgcg gcggctgcat agtggatcgc cttcagcggc
     3781 aggcgatgcg cgaaggcgtt accgagaaag atgaccggga tgttcgcggc catcatgccg
     3841 caggtcgttc cggacaccac cgccagcaga ttggagtacg ccgctgccag ggccacggtc
     3901 gcgatctggg tcttgtctcc aatttccgcg atgaagaacg cgatcagcgt cgtcacgaag
     3961 gcactggcgc cggtgacctc gccctgcttc tcatccagct tgtcgggttt cagggtccat
     4021 agcgccatgg caatcatgct gacgccgacc atcaggtcca ggcgcgtcgg cgtcaacagt
     4081 ttgcccgccc agacaccgac caggcctgcc agcacgtgac tggccagggt tgcgcaaaag
     4141 gcgccggcca cgatcggcca gggtctgcgg aatttcgcgg ccaggaccag cgccagcagt
     4201 tgggtgcgat cgcccatctc cgccgtggca acgctggaaa gggagatcag aaaggcctcc
     4261 ataccctaag acgcccgccg gtcgcgcact tttaatggca ttggactacc atcgccttct
     4321 ggccaaaacc ggcatggatg atacggggga gtcagccacc atgaatgaca aactgcgacg
     4381 ggcgactccg attgcagtat cggccctggc gctcggtatc agctcggtga gcgtgcaggc
     4441 accggctacg aaataattcc tctgcaggat tcgaggagca accatgggac tttttgacgg
     4501 aataatcggc ggcctggttg gcggcgagat ggcctcggtc gtcaaccagc tgatcgaaag
     4561 acacggcggc gtccagggca tcatcatgca gatggagcaa cagggactcg gcccgacggt
     4621 gaagtcctgg gtgggaaccg gcctgaatca gccgatctca ccgacgcagg ttcaccaggc
     4681 tttcggcccg cagatcctca gcgagctcgc ggcgaagacc ggactgaatc cgcaggagct
     4741 ggcgcagaag ctgtcacagg tattgccgca ggtggtggac aagctcacgc ccaacggcca
     4801 ggtgcccagc cactagaccg atcggatccg gaccgtcact cgacgccgcc ggcatccccc
     4861 ggccgtcgat tgacggcaac gacggtcgct ttcggaaaga agttgaattc cgaagcgctg
     4921 gcccaagtgt aggcgcccat ggcgcgaccc acgatcagat cgccggcctt gagttccggc
     4981 agcatcagat tctcagcgat gacatcaatg ctgtcgcagg tcggacctgc cagcaccgaa
     5041 ggcagcagcg gtacgccctc gcgcagcgac tccaccgggt agcgcgagtg atcgtagagc
     5101 tgaccgctgt acgagccgta caaaccgtcg tcgaggtagt accaccagtg cccttcgcgc
     5161 tgcgcgcgac ccatgaccgt cgacacaccg atcgcgctcg ggccggcaat gtagcgcccc
     5221 ggttcggcaa tcacgcgcac ccgcttgggc agcttggcca gcgcggttcg aatcggcgca
     5281 cagaatcggc gaatctccgc agtgcgtttc aaatactcga tcgggaaacc gccgccgatg
     5341 tccagcgtat cgagcgtgcc gagccgctcg cgccgtgccc tcgccagcag gctcgagcac
     5401 acctcgatgg cctcgacatg cttgagcgga tcgggcgcct gcgagccgac gtgaaacgag
     5461 aatccctgta catccacgcc cagtgacgcc gccagtcgcg ccagttcaac ggcaccctcc
     5521 ggatcgcagc caaacttgcg cgacagatcg cacaccgcgc ccggcgcccg gaacgacacg
     5581 cgcagcagca gcgcggtgcg ctcaccgtag ggcttgaact tgcgcacctc atccggattg
     5641 tccgccacga acacccgtac cccgtattcg agggcgttgt tgatgtcgcg ttcgcgcttg
     5701 atcgggtgcg tatgaatgca gcggttggcc tgcgccccca gggcctgcgc gagctgcacc
     5761 tcgcccgtgg tcgcaagatc gagccagccg ccggcctcca cgatcgcccg caccaccgca
     5821 gcatgcggca agggtttcag tgcatagtgc agatccacgc ctggcagggc gccgcgcagc
     5881 ttgcggtact gcgaacgcac gcgcgcgcag tcgatgatca gcagcggtga accaaattcg
     5941 cgcaccagcc gccggatctg ctctggctcg aacgggtcga tgggacgcgg catcagcttg
     6001 agaagaccct cacggcagca cgcccagtgc gagagcgtcg ctgttgtccg tcagctcctc
     6061 actactggct gccgcggccc gttgcagccg gtcacgcgca gcgtcccagc gttcatcgcc
     6121 cgggacttcc tgcaccagaa ttcgggcaca gccgactttg tcgagcgtgc gcaaatgcgc
     6181 gtacagcacg tgcgcatagg cctcggcgcg ttgtccggca ttgatccagg tcaacagccg
     6241 ataggttccc agcggcggcc gcatcgccaa taccccagtg cgctggccgc tctcagccat
     6301 attcgccaca acccgctcca gatcatcgcc cggcacgatc gacaacggtg tcgtcggggc
     6361 atagtgcacg cgctggctgc caggtacccg cggtgcatac ggcgacgcgt ccaccagaac
     6421 ctctcccacg acgcgctgca gttgcgacag cgttaccgcc ccgggacgca acagccgcac
     6481 agtcccctca aggcacgcca cgatcgtgga ctcgagtcca agtttgcact cacccccgtc
     6541 caagaccacc cgtacggtgc taccgaattc atcgcgcacg tgttcagccc gcgtgggact
     6601 gacatgcccg tagcgattgg ctgagggtgc cgcaattcca ccaccgaacg cagtcagcag
     6661 ttgctgcgcc atggggtgcg cgggcacccg aacggcgacc gtgtcctgcc cgcccgtgat
     6721 cagcgtatgg acgttctcgg ctcgcggcag taccagcgtc agcggacccg gccagaagcg
     6781 ctcggccagc attgcagcag cgacgggaac ctcgcgcgcc caacgcgtca ggaaccgcgg
     6841 gctgtcgatg tgcacaatca gcggatgcga ggtaggtctt tgtttgagtt cgaatacccg
     6901 gcgtaaggcg gccggatgcc cggcgtgcgc acccagcccg taaaccgtct cagtcgggaa
     6961 cgccaccagc tcgccgtcgc gcaatgcctg cacggcggcg tccagctcga aatgcgtggc
     7021 gcgaacgacg ggtaccatac ccagaattgt accggccgcg cgttgatgct attcgagaga
     7081 ttgtcgctcc caccccaccg cagtgcggtt aagttcataa gcgggccagt agcgccgatc
     7141 gagcttcaaa gtgatgactt ccggcgctcc gttccaggac aaggtcttga ttcgcaaaga
     7201 cgagcggtcc gggcgaccac tcacttgtcg taggaaagca tcaagatcgg gggtcagttc
     7261 accatccact tcgacaatgc ggcggcccgg aaccaggcca aagcgcgacg cgggcgaacc
     7321 aaactggaag aatgcgacat aaacccccac cggcgggatg ccacgctgcg cactgatcgc
     7381 tcgatgtggg gcttgcagcg tcgcccccgc ccactccacc acgcgatcga tgtcgttgcc
     7441 cgagaggctt gcggtcttga cctgcagatt cttctcgcca tccgagcgcc agacagtcgc
     7501 cgtgacctga tccttgttcg ccaccgcgcg ctcgacttcg cgaaattggg tcagcggctt
     7561 gtcatcgatc gccagcagga tgtcaccggt ctgcagcaca cgcgcggcat cggatccggc
     7621 caccagccgc gagacactca gcacttggcg cgctgacgga ttggtctgcg cgattcggcg
     7681 cacccaggca tcgctcaacc ccagccgccg cgccacagcc aacggctgtt cgctgaattc
     7741 cgcctccagt gaatgcaacg gccggttgtc tcgcacgata tccagtgtct cggcgagcag
     7801 atcgctcgac agcccgcgat tctcctggat gagttcgcga ccgttatcgg aggcgaagct
     7861 ggcccacaga ccgcggacct tgccgctatt gtccgcgagc actccgacat agtcgtccgg
     7921 cgcattgacc aacgaggcca cctgcaggtt gctctcgcgg aactgcactg agcgcgatag
     7981 cggcaattgc agcggatcga catccgcaat catcgtgctg cgcgtcttga tatcgccgtt
     8041 tccgtccatg ccgatcacat tcacggcttc acccggcttc agcggcgcac tgtcgagctt
     8101 cgcgctcctg atcggggtcg taccgatcag cttcgggtcg tagtgaatca ccgtcaggtt
     8161 gtgcagtgga tgcacataaa cgatctgacc tggaatctcg agcgtgccgg cgaaggtcag
     8221 tcgcacgtcg ccgatcgaca ccggtaccgt gttgcgatcg gtaacgatca ggccgcgctc
     8281 ggcatcgacg atcaccccaa cgccgtgata attgcgctcg gtgacacctg agaccgagta
     8341 cggcatgtcg aaggtgacga ataccagtga cggcgcaatc tccgccgcgt acttgtcgtc
     8401 cacgcgcagc ggctgcgcgg aggccggtgc cggagtctcc acgttggccg gcgtcggcag
     8461 gtcggtgcag ttccagtaac cgactttgtc gtcacgctgg cacgctcgcg ccggatacca
     8521 gcgccgatca atgcggatcg aacgcagctg actcgaggtc gcatcgtcga gcgtgacata
     8581 gcgcacggtg acacgcgc
//
LOCUS       DATMRM010000119         3589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_54591, whole genome shotgun sequence.
ACCESSION   DATMRM010000119 DATMRM010000000
VERSION     DATMRM010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54591"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VNZ06_03490"
     CDS             complement(<1..250)
                     /locus_tag="VNZ06_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HWW19845.1"
                     /translation="MTAVRRRRTGGTADATRISLGGLDLNALRRIARAPVRVSLSRAD
                     LRKLTASAAIVKRIVDREQVAYGINTGFGLLAQTRIAHE"
     gene            complement(247..1923)
                     /gene="hutU"
                     /locus_tag="VNZ06_03495"
     CDS             complement(247..1923)
                     /gene="hutU"
                     /locus_tag="VNZ06_03495"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HWW19846.1"
                     /translation="MDVLTRTDENRTVRAQRGSQLQTRSWLTEAALRMLRNNLDPEVA
                     ERPHDLVVYGGIGRAARSWECYDQIVAALKTLGDDETLLIQSGKPVGVFRTHTDAPRV
                     LLANSNLVPHWATWEHFNELDRKGLMMFGQMTAGSWIYIGSQGIVQGTYETFVEMGRQ
                     HYSGDLHGRWILTAGLGGMGGAQPLAATMAGASMLAIECQPGRLQKRIETGYLDVSVR
                     KLDEALALIQRSVADRKPLSVGLLGNAAEVLPELLRRGVRPDAVTDQTSAHDPVNGYL
                     PAHWTVEDWESAKLTNPARVAAAARQSMAAHVRAMLEFHKQGVPTFDYGNNIRQMALL
                     EGVKNAFAFPGFVPAYVRPLFCRGIGPFRWVALSGDPEDIRRTDAKVKELIPGDAHLH
                     RWLDMAQQRIHFQGLPARICWVGLGQRHRLGLAFNQMVARGELKAPIVIGRDHLDAGS
                     VASPNRETEAMRDGSDAVSDWPLLNALLNTASGATWVSIHHGGGVGMGYSQHAGLVIV
                     CDGTPAAGRRIERVLWNDPASGVMRHADAGYPEAIECAHENQLNLPMVNQ"
     gene            complement(1936..3291)
                     /locus_tag="VNZ06_03500"
     CDS             complement(1936..3291)
                     /locus_tag="VNZ06_03500"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HWW19847.1"
                     /translation="MELLFERALLPDGIARRVAVRIADDGTLRTIATDVQSSAALVVR
                     GLAVSGMPNLHSHAFQRGMAGAAEVSAAADNSFWGWRETMYRFVERLRPEDVAAIAAM
                     AYVEMLEAGYTAVAEFHYLHHQPDGSPYPDRTAMAVAVRAAAQQAGIRQLLLPCLYQV
                     SGFGPRPLLERQRRFALATDQFLRLFAQLRAQSTPLHTIGVALHSLRAVPMASLRDTV
                     ASLPANLPVHIHICEQQREVDECLKFVGRRPIEYLLDSVALSPRWCLVHATHAQPSEL
                     NAVVQAGAVIGLCPTTEANLGDGRFALDDFVDLSGRFGIGSDSQVSIDPCEELRTAEY
                     ALRLHKQRRGLVSVGSQGHCGTALYTLASAGGAQALGIQAGELSVGKLADIVVLDTGR
                     PQFAGLPDEALMDAHVFAPRPGAVRDVMVAGQWVVRDGRHVQREPVSRAYQRSVQALL
                     R"
BASE COUNT          638 a         1182 c         1130 g          639 t
ORIGIN      
        1 gctcgtgcgc gatgcgtgtt tgtgcgagca ggccaaagcc cgtattgatg ccgtaggcga
       61 cctgttcgcg atcgacgatg cgtttgacga tggcggcgct cgccgtgagc ttgcgcaggt
      121 cggcgcggct gaggctcacg cgcaccggtg cgcgcgcgat gcgacgcaag gcgttcagat
      181 cgagtccgcc gagcgagatg cgtgttgcgt cggcagtgcc gccggtgcgg cgtctgcgga
      241 ctgcagtcac tgattcacca tcggcagatt gagctggttc tcgtgagcgc actcgatcgc
      301 ttcggggtag cctgcgtccg cgtgccgcat cacgccgctg gccggatcgt tccaaagtac
      361 tcgttcgatc ctgcgcccgg ccgcgggggt accatcacag acgatcacga ggcccgcgtg
      421 ctgtgagtag cccatgccca ctccgccgcc atgatgaatc gagacccagg tcgcaccgct
      481 ggcggtattc agcagcgcgt tcaacagcgg ccagtccgac accgcgtccg atccatcccg
      541 catcgcctcg gtttcgcgat tcgggctggc gactgagccg gcgtccagat gatcgcgtcc
      601 gatgacgatc ggtgccttca actcgccacg ggccaccatc tgattgaatg ccagacccag
      661 ccggtgccgc tggccgagcc caacccagca gatgcgcgcc ggcaatccct ggaagtgaat
      721 gcgctgttgc gccatgtcga gccagcgatg caggtgagca tctcccggga tcaattcctt
      781 cactttggca tcggtgcggc ggatatcttc cggatcgcct gacagggcaa cccagcgaaa
      841 cggcccgatg ccgcgacaga acagcggtct gacataggcg ggaacgaagc ccggaaacgc
      901 gaacgcgttc ttgacgcctt caagcagagc catctggcgg atattgttgc cgtagtcgaa
      961 ggtcggcacc ccctgcttgt ggaattccag catcgcgcgc acatgcgcag ccatcgattg
     1021 ccgcgctgct gcggcgacgc gcgcggggtt cgtgagcttc gctgactccc aatcctccac
     1081 tgtccagtgt gcgggcaagt atccattcac ggggtcatgc gcggaggtct gatccgtgac
     1141 cgcatccggc cgcacgcccc ggcgcagcag ctccggcagc acttcggcgg cgttacccag
     1201 cagaccgacc gacaggggct tgcgatctgc gaccgagcgc tgaatcaatg ccagcgcttc
     1261 atcgagcttg cgtacgctga catcgagata tccggtctca atgcgtttct gcaagcggcc
     1321 cggctggcac tcgatggcca gcatcgaggc gccggccatg gtggcagcca gcggctgagc
     1381 cccgcccatg ccgcccaatc cggcggtcag tatccagcgc ccgtgcaggt caccactgta
     1441 gtgctgccga cccatctcga caaaggtctc gtaggtgcct tgaacgatgc cctgactgcc
     1501 gatgtagatc cacgatccgg ccgtcatctg gccaaacatc atcaggcctt tgcgatcgag
     1561 ctcgttgaag tgttcccatg tggcccagtg gggtaccaga ttggaatttg cgagcagcac
     1621 gcgtggcgca tccgtgtgcg tgcgaaagac accgacgggt ttgcccgact ggatcagcag
     1681 tgtttcatcg tccccgagtg tcttcagcgc ggcgacgatc tggtcatagc attcccagct
     1741 gcgggcggcc cgaccgatgc caccgtagac caccagatca tgcggtcgct cggcgacctc
     1801 gggatcgagg ttgtttcgca acatcctcaa cgctgcttcg gtcagccacg atctggtctg
     1861 cagctggcta ccgcgttgtg ccctgacggt acggttctca tcggtccggg tcagtacgtc
     1921 catgagtatc ccccttcatc gcaacagagc ctgcacgctg cgctgatatg cccgactgac
     1981 cggttcgcgc tgaacatgtc gaccgtcgcg aaccacccac tgacccgcca ccatcacgtc
     2041 gcgcacggct cccgggcgcg gtgcaaacac gtgcgcgtcc atcagcgcct catcgggcag
     2101 accggcaaac tgcggccgcc cggtgtcgag aacgacgata tcggcaagct ttcccactga
     2161 cagctcaccg gcctgaatgc ccagcgcctg tgcgccgccg gcgcttgcga gcgtgtagag
     2221 tgcggttccg caatgtcctt gcgagccgac gctgaccagt ccgcgacgct gcttgtgcag
     2281 tcgcagggcg tactcggccg tgcgcagctc ttcacacgga tcgatcgaca cctgactgtc
     2341 cgaaccaatt ccgaaccgtc cactcaggtc cacgaagtca tcgagcgcaa agcggccatc
     2401 gcccagattg gcctcggtcg tcggacacag tccaatgacc gcgccggcct gaacgactgc
     2461 gttcagctcg gacggctggg cgtgggtggc gtgcaccagg caccagcgcg gcgacagggc
     2521 gaccgagtcg agcaggtact cgatcggtcg ccgaccgaca aacttcaggc attcatccac
     2581 ctcgcgctgc tgttcgcaga tatgaatgtg caccggtagg ttcgccggca agctcgcgac
     2641 ggtgtctcgc agcgatgcca tcggcaccgc gcgcaggctg tgcagcgcga cacctatcgt
     2701 gtgcagtggc gttgactggg cacgcagctg cgcgaacagc cgcaggaact gatcggtggc
     2761 aagcgcgaaa cggcgctgcc gttcgagcag cggtcgcggt ccgaatcctg acacctgata
     2821 cagacacggc agcagcagct gtcggatgcc ggcctgctgc gcggccgcgc gcaccgccac
     2881 ggccatcgcc gtgcggtcgg ggtaggggct gccatcgggc tgatggtgca ggtagtgaaa
     2941 ctcggcaacc gcggtatagc ccgcttccag catttccacg tacgccattg cggcgatggc
     3001 cgcgacatcc tccggccgca agcgctcgac gaagcggtac atggtctcgc gccagcccca
     3061 gaacgagttg tccgccgcgg ccgatacctc ggcggccccg gccatgcctc gctgaaaggc
     3121 atggctgtgg agattgggca taccgctgac ggccaggccc cggaccacaa gcgcggcact
     3181 ggattgcaca tcggttgcga tcgtacgcag cgtcccgtcg tcggcgatcc ggacggcgac
     3241 cctgcgggca atcccgtcag gaagcagcgc acgttcaaac agcagctcca cagtgcctcc
     3301 agtgtgcgcg gtcagggatc gggatgaaag cgcaagctaa acataacgat acttgtatag
     3361 gcaagttcac ggatgcggcg cactcgtccg gtttttcggc ctaaaatggc gcttgcgcat
     3421 gcgggattga cttgtataga cgggtgagga catttgagcg cagatacgca agccccgtgg
     3481 gatcggctgt gggtcaactg ccacctcgcc accatggccg gcgccgatcc attctacggc
     3541 gtgatcgatg acggcgcggt tgccacgcgc gctggccgca ttgtctggg
//
LOCUS       DATMRM010000120         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1627069, whole genome shotgun sequence.
ACCESSION   DATMRM010000120 DATMRM010000000
VERSION     DATMRM010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627069"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VNZ06_03505"
     CDS             complement(<1..222)
                     /locus_tag="VNZ06_03505"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HWW19848.1"
                     /translation="MSSAGEALDALLALMCALRAPDGCPWDREQTFASIAPFTIEEAY
                     EVADAIGRGDLDRLRDELGDLLFQVVFHAR"
     gene            complement(219..881)
                     /locus_tag="VNZ06_03510"
     CDS             complement(219..881)
                     /locus_tag="VNZ06_03510"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HWW19849.1"
                     /translation="MPAIAPQLPADWRERLHSGLLARPDVELENVRFGGLASGSASVR
                     AHLKEGWPPTMRAAAVLVGIVDRSETPSLLMTVRAGHLRHHAGQISFPGGRLEPFDAD
                     IAAAAIRETHEEIGIDVDLIRPIGFLSDHVVQTGFRITPVVALIRPGFTLRADSTEVA
                     DVFELPLKLALAIESYRARQRTVRDVQVEVWELPFGEYQIWGATAGILLNLREVISGA
                     HA"
     gene            complement(884..>2207)
                     /locus_tag="VNZ06_03515"
     CDS             complement(884..>2207)
                     /locus_tag="VNZ06_03515"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HWW19850.1"
                     /translation="QRAVNNFPISGLTMPRAFIRALGLIKQAAAAANVQLGLLDRKLG
                     EAIQHCAAEVAGGRHDRQFPIDVFQTGSGTSSNMNANEVIAHLTVRGGTAVHPNDHVN
                     MCQSSNDVIPTAIHVSAALMLREELLPALTHLAEVIRARESELGDVVTTGRTHLMDAM
                     PVTLGQELSGWRAQIENGLARLRAVEPRLLALAQGGTAVGTGINAHPDFAATVCAGLR
                     ELTGIAFQPNSNFFESLSAQDTAVELSGQLRVVAVSLMKIANDLRWMNSGPLAGLGEI
                     ALPALQPGSSIMPGKVNPVAAEATAMVAAQVMGNDVTITIAGQSGNFQLNVMLPVIAH
                     NLLESIHLLSNVSRVLADSAIKGFTVNRNRLDEAIDRNPILVTALNSVIGYEKGTAIA
                     KKAYAEGKPIRDVAEAMTHLSRPELERLLDPTELTRGGIKAGGGSGGG"
BASE COUNT          389 a          737 c          669 g          412 t
ORIGIN      
        1 gcgcgcatgg aacactacct ggaatagcag gtctccgagc tcatcgcgca gtcggtccag
       61 atcgccgcgt ccgatcgcat cggccacttc atatgcctct tcgatcgtga acggcgcgat
      121 gctcgcgaat gtctgctcac ggtcccatgg acaaccatca ggcgcgcgta gtgcgcacat
      181 caaggcgagc agggcgtcca gcgcttcgcc tgcactgctc acgcgtgcgc tccgctgatg
      241 acttcgcgca ggttcagcag aatgccggcg gtggcccccc agatctgata ctccccgaag
      301 ggcagctccc acacctccac ctgaacatcg cgcacggtgc gctgccgggc acgatagctt
      361 tcgatcgcca gtgcgagctt cagcggcagt tcgaacacgt cggccacttc ggtgctgtcg
      421 gctctaagcg taaaacccgg gcgaatcaaa gccacgaccg gtgtgatgcg aaatccggtt
      481 tgcacgacgt ggtcggagag aaacccaatc ggccgaatga gatcgacgtc gatgccgatt
      541 tcctcatgag tctcgcgaat cgccgcggcg gcgatgtcgg cgtcaaaggg ctcgagccgt
      601 ccgcctggaa agctgatctg gccggcgtgg tgccgcaaat gccccgcgcg cacggtcatc
      661 agcagcgaag gcgtttcgct tcgatcgacg attcctacca gcactgcggc cgcacgcatc
      721 gtcggcggcc agccttcctt gaggtgcgcc cgcacgctcg ccgaaccaga agccaggccg
      781 ccgaagcgca cgttctccag ctcgacatcg ggtctggcca gcagtccgct gtgcaggcgc
      841 tctcgccagt ctgcgggcaa ctgcggtgca atcgctggca acgttaaccc ccgccgctac
      901 cgccgccggc cttgatgccg ccacgggtca gctcggtggg atccagcaac cgctcgagct
      961 ccgggcgcga cagatgggtc atcgcctcgg cgacgtcgcg gatcggcttg ccctcggcgt
     1021 aggccttctt cgcgatcgcc gtgcccttct cgtaaccgat cacggaattc agcgcggtga
     1081 ccagaatcgg gttgcggtcg atcgcctcgt ccaggcggtt gcgattgacc gtaaaaccct
     1141 tgattgcgct atccgccagc acgcgtgaga cgttgctcaa cagatgaatg ctctcgagca
     1201 ggttatgggc gatcaccggc agcatgacgt tgagctggaa gttaccggac tggccggcga
     1261 tcgtaatcgt cacgtcgttg cccatcacct gagccgccac cattgcggtc gcctctgccg
     1321 cgaccggatt gaccttgccc ggcatgatgc tgctgcccgg ctgcaacgcc ggaagtgcga
     1381 tctcccccag gcctgcgagc gggccgctgt tcatccaacg caggtcattg gcgattttca
     1441 tgaggctgac cgccacgacc cggagctgac cggagagctc gacggccgtg tcctgcgccg
     1501 aaagtgattc gaaaaagttg gagttcggct gaaacgcgat gccggtgagc tcccgcagcc
     1561 cggcgcacac cgtggcggca aagtccgggt gcgcgttgat acccgtaccc accgcggtac
     1621 ctccctgcgc gagtgccagc aggcgcggct ccactgcgcg cagccgtgcc aggccgtttt
     1681 cgatctgtgc gcgccacccc gacagttcct gtcccaaggt caccggcatc gcgtccatca
     1741 ggtgagtgcg accggtcgtt accacatcgc ccagttcgga ttctcttgca cgaatgactt
     1801 ccgccagatg cgtcagagcc ggcaatagct cttcgcgcag catcaacgcc gcactcacgt
     1861 gaatcgcggt tggaatgaca tcgttgctgc tctggcacat gttgacgtga tcgttggggt
     1921 gcactgccgt acccccccgt acggtcagat gcgcgatcac ctcgttggcg ttcatgttgc
     1981 tgctggtacc cgagccggtc tgaaaaacat caatcgggaa ttgccgatca tgtctgccgc
     2041 ccgctacctc ggccgcgcag tgctgaatcg cctcgccgag ctttcgatcg agcagtccga
     2101 gctgcacgtt ggccgccgcc gccgcctgtt tgatgagacc gagcgcgcga atgaatgcgc
     2161 gcggcatagt cagcccgctg ataggaaagt tattgacggc gcgttgc
//
LOCUS       DATMRM010000121         4462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_54857, whole genome shotgun sequence.
ACCESSION   DATMRM010000121 DATMRM010000000
VERSION     DATMRM010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54857"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="VNZ06_03520"
     CDS             <1..169
                     /locus_tag="VNZ06_03520"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004644206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HWW19851.1"
                     /translation="ALKFDREQARGFCLNIAAGTAVRFEPGQQRDVELVALAGDRKVY
                     GFQGKIMGALS"
     gene            166..1869
                     /gene="ureC"
                     /locus_tag="VNZ06_03525"
     CDS             166..1869
                     /gene="ureC"
                     /locus_tag="VNZ06_03525"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HWW19852.1"
                     /translation="MTTIDRRAYAEMFGPTTGDRVRLGDTDLWIEVERDHTVYGEEVK
                     FGGGKVIRDGMGQSQRTASQVVDTIITNALILDHWGIVKADVGLKDGRIASIGKAGNP
                     DVQPGVTIAIGAGTEVIAGEGLILTAGAIDAHIHLICPQQVEDALMSGVTTMIGGGTG
                     PATGTAATTCTPGPWYIGRMYQALEELPMNFGLLGKGNASLPEALEEQIRAGVMGLKL
                     HEDWGTTPQAIDTCLSVAERFDVQVAIHTDTLNESGFVEDTIAAFKGRAIHTYHTEGA
                     GGGHAPDIMTVCGVPHVLPSSTNPTRPYTVNTIDEHLDMLMVCHHLDPGIPEDVAFAE
                     SRIRRETIAAEDILHDLGAFSMISSDSQAMGRVGEVITRSWQTAHKMKQQRGALPQDS
                     ARCDNFRARRYIAKYTINPAITHGIAHEVGSIEAGKLADLVLWRPAFFGAKPAMIIKG
                     GMIVAAPMGDPNASIPTPQPVHYRPMFGALGGARSATSVSFVSQAAIDGGIGARLQLR
                     KRLVPVLGTRTLRKTDMVLNDYLPIMEVDPQTYEVRADGQRLACEPASVLPLAQRYFL
                     F"
     gene            1893..2360
                     /gene="ureE"
                     /locus_tag="VNZ06_03530"
     CDS             1893..2360
                     /gene="ureE"
                     /locus_tag="VNZ06_03530"
                     /inference="COORDINATES: protein motif:HMM:NF009751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HWW19853.1"
                     /translation="MLTISRRLQIPASPNARLVLPYDLRSRSRFRAQLLGGEEVGVIL
                     GRGQVLRGGDLLLADDGRVVEIAAAAESVSSVYSADMQSLARAAYHLGNRHVTLQIGQ
                     GWLRYRHDHVLDDMLQGLGLKVQVEQAPFEPEGGAYHTGGHSHGHDENHGHSP"
     gene            2344..3018
                     /locus_tag="VNZ06_03535"
     CDS             2344..3018
                     /locus_tag="VNZ06_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HWW19854.1"
                     /translation="MDTVPESLLRLCHLVSPALPVGAYAYSQGLEFAVQAGWVHDEDS
                     TLEWLRGLSCHAIGTLDLPILARLYRAFMTADAAAVRGWNGQLLASRETLELREEDAH
                     LGRSLARVLVELRLPQASPWLQVEVGFATLFALAATHWRIALTDTLCGYLWAWTENQV
                     LAAVKLVPLGQSAGQRLLYRLIESMPGIAERALRLTDHEIGVSAFSQVLASSLHESQY
                     TRLFRS"
     gene            3071..3865
                     /locus_tag="VNZ06_03540"
     CDS             3071..3865
                     /locus_tag="VNZ06_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19855.1"
                     /translation="MGAVLDNAIELYRHAFRSCWVISLVGTVLTTAVRLRADMDMPNV
                     SFGGKNLQQFGADLVQALQHMNGGVGSLLNNLLVLLLELVLYGSLFAQMHHVAAHARP
                     LATLDALILGVRRLPGMVLSSVIFMVAVFVGFVLFLIPGFYLWGKLEFWVAAAFADDA
                     GAIGGLGRSWEITTGNWWRSATAVTVALIIVVVLNSGADVVGLILLAFTHDLTTLLLV
                     TQTFQGIAAVFVLPMLPAAALAIYYDLKLRREGDDLLIRANSLQTA"
     gene            3870..>4462
                     /locus_tag="VNZ06_03545"
     CDS             3870..>4462
                     /locus_tag="VNZ06_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19856.1"
                     /translation="MRAWLALSLTATAWLWVAPCLAVQKADIDYLSGCLSGLPAREKP
                     GLEHLQPHCPGLKRSLEDAGLIGQLPQGWEQRLRPSGLGEISQSLQRYQGEPQSKAPD
                     TSSVGAIAQALRTEHKQPPKTWWQRLGEWLQKLLTRRGNSGAGLLSRVLDRLADVMTD
                     RVRTVLFYSCVALVVLFVGLIVWREARAAGMGRRAAPP"
BASE COUNT          779 a         1322 c         1469 g          892 t
ORIGIN      
        1 agccctgaaa ttcgaccgcg aacaggcccg tgggttttgt ctcaatatcg ctgccggtac
       61 ggcggtgcgc ttcgagcccg gccagcagcg cgatgtggag ctggtcgcgc tcgcgggcga
      121 tcgcaaggtc tatggctttc agggaaagat catgggcgcg ctgtcatgac gacgatcgac
      181 cgtcgcgcct atgccgaaat gtttggcccg accaccgggg atcgggtgcg tctgggggac
      241 accgatttgt ggatcgaggt cgaacgtgat cacaccgtct acggagaaga ggtcaagttc
      301 ggcggcggca aggttattcg cgacgggatg ggccagagcc agcgaaccgc ctcgcaggtg
      361 gttgacacga tcatcacgaa tgcgctgatt ctggatcatt ggggtatcgt caaagcggac
      421 gtcgggctca aggacggccg catcgcctcg atcggtaagg ccggcaatcc ggatgtgcag
      481 cccggcgtga ccatcgcgat cggtgccggc accgaagtca tcgccggtga aggactgatc
      541 ctcactgctg gcgccatcga cgcgcacatt catctgatct gtccgcagca ggttgaggat
      601 gcgctcatga gcggcgtgac cacaatgatt ggcggcggca ccggtccggc gaccggaacc
      661 gcggccacga cctgcacgcc cgggccgtgg tatatcggcc gaatgtatca ggcgctggag
      721 gagttgccga tgaacttcgg tttgctcggc aagggcaacg ccagcctgcc ggaagcgctc
      781 gaggagcaga tccgcgcggg cgtcatgggc ctgaagctgc acgaggattg gggcacgacc
      841 ccgcaggcga tcgacacctg cctgagtgtc gccgagcgtt tcgacgtgca ggtcgcgatt
      901 cacaccgaca cgctcaacga gagcggcttc gttgaagaca ccatcgccgc gttcaagggt
      961 cgggccatcc atacctatca caccgagggt gccggcggtg gacacgcacc cgacatcatg
     1021 acggtctgcg gtgtccctca cgtgttgccg tcatcgacca atccgacccg gccctacacg
     1081 gtcaacacga tcgatgaaca tctggacatg ctgatggtgt gtcaccatct ggatccgggg
     1141 attcccgagg acgttgcctt cgcggagtcg cgcatccgcc gcgagaccat tgcggcggaa
     1201 gacatcctgc acgatctggg cgcgttctcg atgatctcct ccgactcaca ggcgatgggc
     1261 cgggtcggcg aggtcatcac acgcagctgg cagacggctc acaaaatgaa gcagcagcgc
     1321 ggcgcgctac ctcaggacag cgcgcgctgc gacaattttc gcgcgcggcg ctatatcgcc
     1381 aagtacacca tcaatccggc gatcacgcat ggcatcgcgc atgaggttgg ctcgatcgag
     1441 gctggcaaac tcgcggacct ggtgctctgg cgaccggcgt tcttcggtgc gaagccggcg
     1501 atgatcatca agggcggcat gatcgtcgcg gcgccgatgg gtgatcccaa cgcgtcgatt
     1561 ccaacgcctc agcccgtgca ctatcgaccg atgttcggtg ccctgggagg ggcgcgcagt
     1621 gcgacgtcgg tgagtttcgt ttcgcaggcg gccatcgacg gcggtatcgg tgcgcggctt
     1681 cagctgcgca agcgtctggt acccgtactg ggcacgcgca ccctacgcaa gaccgacatg
     1741 gtgctgaatg actacctgcc gatcatggag gtggatcctc agacctatga agtacgggcc
     1801 gacgggcaac ggctggcctg cgaaccggcc agcgtgctgc cactggcgca gcgctacttc
     1861 ctgttttgaa gaccggacgg aatcggtaac tcatgttgac gatttcgcgt cgtttgcaga
     1921 ttccggcatc gccgaatgcg cgtctggtat tgccctatga tcttcgaagt cgtagccgtt
     1981 tccgcgctca gctgctcggc ggcgaagagg tcggcgtgat actggggcga ggtcaggtct
     2041 tgcgcggcgg tgacttgctg ctggcggacg atggtcgcgt ggtcgaaata gcggctgctg
     2101 cggagagcgt atccagtgtc tacagcgccg acatgcagag cctggcgcgc gcggcatacc
     2161 acctcggcaa tcgtcacgtg acgctgcaga ttggccaggg ctggctgcgt taccggcacg
     2221 atcatgtgtt ggatgacatg ctgcagggct taggattgaa ggtacaggtg gagcaggcgc
     2281 cgttcgagcc ggaaggcggc gcctatcaca ccggcggcca ttcccatggt catgacgaga
     2341 atcatggaca cagtccctga atcgctgctg cgcttgtgtc atctggtcag cccggcgttg
     2401 ccggtaggcg cctatgccta ttcgcaaggg ctggagtttg ccgtacaggc cggctgggtc
     2461 cacgacgagg acagtacgct cgaatggctg cgcggattgt cgtgccacgc gatcgggacc
     2521 ctggatctgc cgatactggc tcggctctac cgggccttca tgacggccga tgctgccgcg
     2581 gttaggggct ggaacgggca gcttctggcc tcgcgtgaga ccctggaact gcgcgaggag
     2641 gatgctcatc tgggtcgctc tctggcgcgc gtgctggtcg aactgagact accgcaggcc
     2701 tcgccgtggc tgcaggtcga ggtcggcttt gcgacactgt ttgcattggc ggccacccac
     2761 tggaggatcg cgctgaccga taccctgtgc ggctatcttt gggcatggac cgagaatcag
     2821 gtcctggcgg cggtcaaact ggtgccgttg gggcagtcag ccggtcagcg cctgctgtat
     2881 cgactcatcg aatcgatgcc ggggattgct gagcgcgcat tgcgacttac cgatcacgaa
     2941 attggcgtca gcgctttctc gcaagtgctg gccagttcgc tgcacgagtc gcagtacacc
     3001 cggctgtttc gttcctagcc tgactcttat actacctgcg atgtatacgc tgcccacgaa
     3061 acctgagccc atgggggctg tcctcgacaa cgccatcgaa ctctaccggc atgccttccg
     3121 ttcgtgctgg gtgatttcgc tcgtgggtac cgttctcacc accgcagtgc gcctgcgcgc
     3181 cgacatggac atgccgaatg tgagctttgg cggcaagaat ctgcagcagt tcggcgccga
     3241 cctcgtacag gctttgcagc atatgaatgg cggtgtcggc tcactgctga acaatttgct
     3301 ggtactgcta ctggagctgg tgttgtacgg ttctttgttc gctcaaatgc atcatgtggc
     3361 cgcgcacgct cgcccactgg ctacccttga cgcgctgatc ctcggagtgc ggcgcctgcc
     3421 gggcatggtg ttgtcatccg tgatcttcat ggtggccgtc tttgtcggct tcgtgctgtt
     3481 cctgataccg ggtttctatc tctgggggaa actcgagttc tgggtggcag ccgcgttcgc
     3541 cgatgacgct ggtgccattg gcgggcttgg acgcagctgg gagatcacga ccggaaactg
     3601 gtggcgatcg gcaacggccg tgacggtcgc actcatcatc gtagtggtac tcaacagcgg
     3661 cgccgatgtt gtggggctca tcctgcttgc ctttacgcac gatctgacca cactgctgct
     3721 ggtcacacag acttttcagg gtatcgcagc ggtctttgta ctaccgatgc tgccggcagc
     3781 ggctttggcc atttactacg acttgaagct gcgccgagag ggtgacgatc tcttgatccg
     3841 cgccaattcg ctgcagacgg cctgaggtcg tgcgggcgtg gctcgcactg agtctgactg
     3901 ccaccgcgtg gctgtgggtc gcgccctgtc tggcggttca gaaggcggac atcgactacc
     3961 tgagcggctg cctctcgggt ctgcctgcgc gcgagaagcc gggactggaa catctccagc
     4021 cacactgtcc tggactcaag cgctcgctcg aggatgctgg attgatcggt cagttgcccc
     4081 aaggctggga gcaaaggctg cgaccgagcg gcctgggcga gatcagccag tcgttgcagc
     4141 ggtatcaggg cgagccgcag agcaaggccc ccgacacgag cagcgtcggc gcgatcgctc
     4201 aggcactgcg caccgagcac aaacaaccac cgaagacctg gtggcagagg ctcggcgagt
     4261 ggctgcaaaa gctgctgacg cgtcgtggca actcgggcgc tggactgctc agtcgggtgc
     4321 tcgatcgtct cgccgacgtg atgaccgatc gggtcaggac agttctcttc tatagttgcg
     4381 tggccctggt cgtactattc gttggattga tcgtgtggcg tgaagcgagg gccgctggca
     4441 tgggtcggcg cgcagcacca cc
//
LOCUS       DATMRM010000122         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1918654, whole genome shotgun sequence.
ACCESSION   DATMRM010000122 DATMRM010000000
VERSION     DATMRM010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918654"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1825)
                     /locus_tag="VNZ06_03550"
     CDS             complement(173..1825)
                     /locus_tag="VNZ06_03550"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HWW19857.1"
                     /translation="MYFRRKQSQGRIYLQIVESHRTGDQVRQRVIATLGRLDELEASG
                     QLDRLLRSGARFVQQAMVLDAARTGDAPAVAVRRIGPPLLFERLWQETGCQSVIAALA
                     RGRGHRFALERAVFLTVLHRLMRGGSDLAADRWRDDYRITGTDDLDLHHLYRAMAWLG
                     EELRSDQQDGATPFSPRCAKDLIEEQLFAHRRDLFSRLDLVFMDTTSLYFEGLGGQSI
                     GQHGYSKDHRPDLRQMILAVLIDGDGRPVCSEMWPGNTADVTSLLPVIDRLRQRFAMG
                     RVCIVADRGMISAETIAALEARGLLYILGVRERTDKLVREVVLGDAAPFVPLVIEKRG
                     RDTDYGAKAVTLGGARYIVCINHQEAAKDAAERVAILAALERQLRRGDKALVGNTGYR
                     RFLKTAGKDHFAIDRAKAAEDARFDGIFALRTNTDLNPLAAMLRYKQLWTVERTFRTA
                     KHLLATRPIFHKLDETIRGHVFCSFLALVLKAELDARIAALGHTGSWPAIIADLDALT
                     ETEIEQDGRRFLLRSTPRHAASLALRAAGVALPPTVQAVAAH"
BASE COUNT          385 a          659 c          697 g          386 t
ORIGIN      
        1 ttgcctcggg cgtaccctgt ggcaagtttg ggccgaacca aaacggtggc aaacagaccc
       61 aggctctccg catccaaacc catcttcaac agtgacttcc agcaagtgat tgaacaggcg
      121 cggcaattcg cgctggcgcg acggcgtggc actacatgtt ggtggagcgt aattagtgcg
      181 cggcgaccgc ctgcacggtt ggcggcaatg ccacgccagc ggcgcgcagt gcgagactgg
      241 cggcgtgtcg gggagtggag cgcaacagga agcgcctgcc atcctgttcg atctcggtct
      301 cagtcagcgc atcaagatcg gcgatgatgg ccggccatga tccggtgtgg ccgagggctg
      361 cgatgcgggc gtccagctcg gccttgagca caagggcgag gaagctgcag aacacatggc
      421 cgcgaatggt ctcgtcgagc ttgtggaaga ttggtcgcgt ggcgagcaga tgcttggcgg
      481 tgcggaaggt ccgctcgacg gtccagagct gcttgtagcg cagcatcgcc gccagcggat
      541 tgagatcggt gttggtgcgc agcgcgaaga tgccatcgaa ccgggcatcc tctgcggcct
      601 tggcgcgatc gatggcgaag tggtccttgc ccgcggtttt gaggaagcgg cgatagccgg
      661 tgttgcccac cagcgctttg tcgccgcgac gcagttggcg ttccagtgcg gccagtatgg
      721 cgacacgctc ggcggcatcc ttcgccgcct cttggtgatt gatgcagacg atgtagcgcg
      781 ccccgcccag cgtgaccgcc ttggcaccgt agtcagtgtc ccggccgcgc ttctcgatca
      841 ccaacggcac gaagggtgcg gcatcgccga gtacgacctc gcgcaccagc ttgtcggtgc
      901 gctcgcgcac gccgaggatg tagagcaggc cgcgtgcttc caaggcggcg atggtctcgg
      961 cgctgatcat gccgcggtcg gcgacaatgc agacgcggcc catggcgaag cgttggcgca
     1021 gccgatcgat caccggcagc aggctggtga catcggcggt gttgcctggc cacatctccg
     1081 agcacaccgg ccggccgtcg ccatcgatca gcacggcgag gatcatctgg cgcagatcgg
     1141 gccggtggtc cttgctgtag ccatgctgtc cgatcgactg gccgcccagg ccttcgaagt
     1201 acaggctggt ggtatccatg aacaccaggt cgagccggct gaacagatcg cgccgatgcg
     1261 cgaacagctg ctcctcgatc agatccttcg cgcagcgcgg tgagaagggt gtggcgccat
     1321 cctgctggtc gctccgcagt tcctcaccca gccaggccat cgcacgatag aggtgatgca
     1381 gatcgagatc gtcggtgccg gtgatgcgat agtcatcacg ccagcgatcg gcggccagat
     1441 ccgagccgcc gcgcatcagt cggtgcagca cggtgaggaa caccgcgcgt tccagcgcga
     1501 aacgatggcc gcgtccgcgc gccagtgcgg cgatgacgct ctggcagccg gtctcctgcc
     1561 acagccgctc gaacagcagt ggcgggccga tgcgtcgcac cgcgacggcg ggagcgtcgc
     1621 cggtcctggc ggcatccagc accatcgctt gctggacgaa ccgcgcacct gagcgcagca
     1681 atcgatcgag ttgtccgctg gcttccagtt catccagccg gcccagtgtc gcgatcaccc
     1741 gctggcgcac ctgatcaccg gtgcggtggc tctccacgat ctgcaggtag atgcgtccct
     1801 gcgactgttt gcggcggaag tacatgcgcg actcgatagg gtgcgcacag attgccgcat
     1861 atcatcgtgc agtcaacaga caatatcatc cgcacgtggc actacatatt cagcgaaatc
     1921 accatggccg ctcaatgaaa tcagagactt ataccatccc tggagaaccc aactgtcgat
     1981 gatgggtata actcgcgtgt agcacatttc gatgccattg tgttcggccg gttaccgagc
     2041 ggcactctgc tgcgcggaag ccacaacttc ttattgcagt ttggggatcg ggctgcggtt
     2101 gaatcgctac cgcacgccat gattctg
//
LOCUS       DATMRM010000123         2739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_991157, whole genome shotgun sequence.
ACCESSION   DATMRM010000123 DATMRM010000000
VERSION     DATMRM010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991157"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VNZ06_03555"
     CDS             <1..594
                     /locus_tag="VNZ06_03555"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWW19858.1"
                     /translation="MSAEDLAAIVRFYAYSPLTAALLPLMLHEADRGNYEPLLSQKKM
                     LADSLGTEISGGMELSVICTEDADLLSSNPQDAATLMGSSTVERIKSACSLWPKGERP
                     ADFHHPFTSALPVLMLEGQYDPVTPPGYGQQMLKNLTHARLLIAAGQGHGVSAVGCMP
                     RLVGKFVDDIDPSKIDAGCLNALGDTPPFADFNGAPP"
     gene            591..1328
                     /locus_tag="VNZ06_03560"
     CDS             591..1328
                     /locus_tag="VNZ06_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWW19859.1"
                     /translation="MIDVQQLHKAFGSKVAVDDVSFSARDGEITGLLGPNGAGKTTTL
                     RMLYTLLKPDRGRISVDGLDSVTRALEVRRRLGVLPDARGLYKRLTARENVEYYAQLH
                     GMEPAMTRRRSESLIQALGMQDIIERRVEGFSQGERVKTAIARALVHDPRNVVLDEPT
                     NGLDVMSTRSLRDFLRQLKAEGRCVLFSTHIMQEVAALCDRIVIIADGRVVADGTPEG
                     LRERAHEQSLEEAFVKIIGTATVGALA"
     gene            1325..2500
                     /locus_tag="VNZ06_03565"
     CDS             1325..2500
                     /locus_tag="VNZ06_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWW19860.1"
                     /translation="MSAAVTVYLKECRESLRDRRVLLNVLLLGPLLGPLLFTIILRVT
                     IGMELDRAERELPVVVIGAERAPNLIEALRQQGLVVRPPVDDVEGAVRDQSVSLALRI
                     GDSFAEDWVAGRAAQVEIIFDSSRRDNGGQVGRLRGMLSYYARRTSAMRLVARGLSPA
                     VITPLVIADRDQATPQARGALLFAMLPYFLILNCFIGGMWLAIDSTAGERERQSLEPL
                     LTNPVPRSRILLGKLGAAASFSFVSLTLGLAAFAVAARFLPVQRLAMSLDIGPRVVAT
                     ILPLMIPLVLLIVVTQIVVSAFAKTFREAQTYLGLLQILPIIPSVLLSVMPINPQPWM
                     YAVPLIGQQLTVMQLLRGEVIAAGSLLWCTLTTLAAMILVFIAARRVYESERLAVNA"
     gene            2493..>2739
                     /locus_tag="VNZ06_03570"
     CDS             2493..>2739
                     /locus_tag="VNZ06_03570"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HWW19861.1"
                     /translation="MPSHLEAFIERYPRLFVLTGAGCSTESGIPDYRGADGEWKRPPP
                     VMFDTFMTNRLTRARYWARSLVGWPRFQAAKPNVAHHA"
BASE COUNT          501 a          823 c          875 g          540 t
ORIGIN      
        1 atgagcgccg aggatctggc ggcgatcgtg cgcttttatg cctatagccc cctgaccgcc
       61 gcgctgttgc cgctgatgct gcacgaggcc gatcggggca actacgagcc gctgctgagt
      121 cagaagaaaa tgctagccga cagcctggga acggagatca gcggcggcat ggaactgtcg
      181 gtgatctgta ccgaggatgc ggacctgctg tcctccaatc cgcaagacgc cgccacgctg
      241 atgggttcga gcacggtcga acgaatcaag agcgcctgtt ccctctggcc caaaggcgag
      301 cgtccggcag atttccatca tccgttcacg tcggcgctgc cggttctgat gttggaaggt
      361 caatacgatc cggtcacgcc tccggggtac ggccaacaga tgctcaagaa cctgacgcat
      421 gcaaggctgc tgatcgcggc cggccagggt catggcgtga gcgccgtcgg ctgcatgccg
      481 agactggtcg gcaagttcgt cgacgatatt gacccgtcga agatcgatgc cggttgtctg
      541 aacgcactcg gcgacacgcc gccattcgcg gacttcaacg gggccccgcc gtgatcgacg
      601 tccaacagct tcacaaggcc ttcggcagca aggtagccgt ggatgacgtg agcttcagtg
      661 cgcgcgatgg ggaaatcact ggtctgctcg gtccaaacgg ggcaggcaag acgaccacgc
      721 tgcgtatgct gtacacactg ctcaagcccg atcggggccg aatcagcgtg gacggcctgg
      781 acagcgtcac gcgagcgctg gaagtacggc gccgattggg cgtgctcccc gatgcacgcg
      841 gactctacaa gcggctgact gcgcgcgaga acgttgaata ctacgcgcag ctgcatggaa
      901 tggaacccgc gatgacccgc cggcgctcgg aaagcctgat tcaggcgctg ggcatgcagg
      961 acatcatcga gcggcgagtc gagggattct cgcagggcga gcgcgtgaaa accgcgattg
     1021 cgcgcgctct ggtgcatgac ccgcgtaacg tcgtgctgga cgaaccaacc aacggtctgg
     1081 acgtcatgtc tacgcgtagc ctgcgagact tcctgcggca actgaaggcc gaggggcgct
     1141 gtgtactgtt ctcgacccac atcatgcagg aggtcgcggc cttgtgtgac cggatcgtga
     1201 tcatcgccga cggacgcgtc gtcgccgacg gtactccgga aggcttgcgc gaacgcgcac
     1261 atgaacagag tctggaagaa gcctttgtca agatcattgg tactgccaca gtcggagcgc
     1321 tcgcatgagt gccgccgtga cggtgtacct caaggagtgt cgcgagagcc tgcgcgaccg
     1381 gcgcgtgctg ctgaatgtgc tgctactcgg cccgttgctc ggacctttgc tgttcacgat
     1441 tattctgcgt gtcacgatcg gaatggagct ggatcgagcc gagcgagagc tgccggtagt
     1501 cgtcatcggc gccgagcggg ccccgaacct gatcgaagca ctcaggcagc agggtctggt
     1561 cgtacgacca ccggttgatg acgtggaagg cgccgtacgc gatcaaagcg tttcactggc
     1621 gctgcgcatc ggtgattcct ttgccgagga ctgggtggcc ggcagggcag cgcaggtaga
     1681 gatcatcttc gattcatcgc gacgcgacaa tggcggtcaa gtcgggcggc tgcgcggcat
     1741 gctctcgtac tatgcaagac gcacctcggc gatgcgattg gtggcgcgcg gactgtcgcc
     1801 agcggtcata acacccttgg tgatcgcgga ccgcgatcag gccacgcccc aggcgcgcgg
     1861 cgccttacta ttcgcgatgc tgccctattt cctgatcctg aactgcttta ttggcggtat
     1921 gtggcttgcg atcgactcga ccgccggcga gcgtgaacgt cagtcgctcg agccactgct
     1981 gaccaatccg gtaccacgta gccgcatcct gctgggtaaa ctgggcgcgg cagctagctt
     2041 cagcttcgtc agcctgacgc ttggactcgc tgcctttgcg gtggccgcgc gctttctgcc
     2101 tgtccagaga cttgcaatga gtctggacat cggaccgcgg gtggttgcaa cgatactgcc
     2161 gctcatgatt ccgctggtgc tgctgatcgt cgtcacacag atcgtcgtct ccgcgttcgc
     2221 caagactttt cgtgaagccc agacctatct cggtctgttg cagatattgc cgatcattcc
     2281 gtcagtccta ttgagtgtca tgccgatcaa tccacaacca tggatgtatg cggtgccgct
     2341 gatcggccaa cagctcacgg tgatgcaatt gcttcgcggc gaggtgatcg ccgcagggtc
     2401 attactgtgg tgcacgttga ctactctggc ggcgatgatc ctcgtgttca ttgccgcccg
     2461 cagggtctac gaatcggagc gtcttgcggt caatgcctag ccatctggaa gcattcatcg
     2521 agcgttatcc tcgcctgttc gtgctgaccg gggccggctg cagtaccgag tcgggcatcc
     2581 cggactatcg cggtgcggat ggcgaatgga agcggccacc gccggtgatg tttgacacct
     2641 tcatgaccaa tcggctcacc cgcgcgcgct actgggcgcg cagtctcgtc ggttggccgc
     2701 gctttcaggc ggcgaaaccg aatgtcgcgc accacgcgc
//
LOCUS       DATMRM010000124         4231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_991289, whole genome shotgun sequence.
ACCESSION   DATMRM010000124 DATMRM010000000
VERSION     DATMRM010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991289"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VNZ06_03575"
     CDS             complement(<1..879)
                     /locus_tag="VNZ06_03575"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HWW19862.1"
                     /translation="MPTSDPQSLGALADEALLEQVQRACFDYFWDGADAITGLAADRR
                     SLSDPNREVPISIGGSGFGIMALLVAAERGWVARAEALERLQPMLQFLGGARSYHGVY
                     SHFMNTRTGETVPLFPKDDGGDLVETSFLMMGLLCARAYFDRESPDERSARLRIDDLW
                     DQVEWSWYTQGRNDVLYWHWSPNYDWQIGHAVRGWNECLITYVLAASSRRFPVDPLVY
                     QRGYAGGDHFRNGRSYYGIELPLGVPGGGPLFFAHYSFCGLDPWQLRDRYADYWQQNV
                     SHVRINRAHCIANPHQH"
     gene            complement(866..2695)
                     /locus_tag="VNZ06_03580"
     CDS             complement(866..2695)
                     /locus_tag="VNZ06_03580"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HWW19863.1"
                     /translation="MARKVTRQTRRSEQAQAHLLPQLRQMTAALWASPVRNALLLLGA
                     CILLDVIATAYGQIRLNRWNQPFYDTLSRRDLSGFLYQLVMFGIIAGSLLALNVGIKW
                     LGEMLKLKLRQGLVLDLIAEWLVPRRAFRLANAGPMGVNPDQRMHEDARHLTELSSDL
                     GIGLLQASILLATFISVLWGISSGFGFQFRGRLLVIPGYMVWAAFIYAGSASLLSYWV
                     GRNLIDANADRYAREADLRFSLVRVSEHIDSVSLAGGETDEARRLRLDLNAVLKAMRR
                     LVWGLTNLTWVTSGYGWFTIVAPILVAAPIYFDSKISFGGLMMAAGAFTQVQSSLRWF
                     VDNFSTLADWRATLLRVASFRRALLDSHEMHAATGQIELAEGPAGKFVIDRLEIASPS
                     GATMLKEPHNELKAGEHVLIIGETGAGKTLLFRALAGLWPWGGGRITRPEQETIRYMP
                     RTPYLPPGSLREVLAYPYEVHEFNQDSYAKALERMQLQRLTPMLDQTKRWEQELNEDE
                     QQQLAFARLLLKAPPWVLIDEVFDSLDEHTLAQVKDVFARELKHSALIHIGRKQQAGP
                     PYARVLHLVNDPDAPRLSRPAAPAEPKSRGKRTAPREPVRADV"
     gene            2758..>4231
                     /locus_tag="VNZ06_03585"
     CDS             2758..>4231
                     /locus_tag="VNZ06_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWW19864.1"
                     /translation="MTRIESLISEMTLTEKLGQLTMTACGYAVTGPVIAGDSTEAIKD
                     GAIGNLLNLVGADHIREMQDLAVKHSRLKIPLLIALDVIHGLRTLFPIPLAEAATFDR
                     EIWERTARAAAQEAAADGIAMTFSPMLDVARDPRWGRIAEGPGEDPWLASQIALAKVR
                     GYQGTDLAAASALAACAKHYCGYGAVSAGRDYASVDISERTLHEVHMPAFRAAVGAGV
                     ASVMPAFTDLAGIPMTVHRALLRDWLRGSYGFNGVIVSDYNSIGELLKHGVAADLTEA
                     ATLALRAGVDIDMMSNAYRTGLPLALERGWVNINEIDAAVRRVLTLKERLGLFDDPYR
                     RGSVPENLSVLSERRQLARRASARSIVMLQNVRASLPLPSSLRRLALLGPLADAAVEM
                     GGCWGAASKPDTHVSVRQGLRAALPETDITYAPGVAINGDDQSQIAEALDLCDRTDAI
                     VLCLGESANMSGEAASRTDLDLPGLQRGFAQAVFESASTHG"
BASE COUNT          784 a         1337 c         1392 g          718 t
ORIGIN      
        1 atgctggtgg ggattggcga tgcagtgcgc gcggttgatg cgtacgtgcg agacattctg
       61 ctgccagtag tccgcatagc ggtcgcgcag ctgccacgga tccagcccgc agaacgagta
      121 gtgcgcaaag aacaacggtc cgccgccggg cactccgagc ggcagctcga tgccgtagta
      181 gctgcggccg ttccggaagt gatcgccccc agcgtagcca cgctgataca ccagcggatc
      241 gacgggaaag cgccgtgagg atgccgcgag cacataggtg atcaggcatt cgttccagcc
      301 gcgcaccgca tggccgatct gccagtcgta gttgggactc caatgccagt acagtacgtc
      361 gttgcgtccc tgggtgtacc agctccactc gacctgatcc cacaggtcat cgatccgcag
      421 acgcgccgag cgctcgtcgg gcgactcgcg atcgaagtac gctcgtgcgc acaacaggcc
      481 catcatcagg aacgaggtct ccaccaggtc gccaccgtca tccttcggaa acagcggtac
      541 ggtctccccg gtgcgggtgt tcatgaagtg cgagtagacg ccgtgatagc tgcgcgcgcc
      601 gcccaggaat tgcagcatgg gctgcaggcg ttccagcgcc tcggcacgcg caacccagcc
      661 gcgttcggcg gcgacgagca gcgccatgat tccgaaaccc gatccgccga tcgagatcgg
      721 cacttcccgg ttcggatcac tcagcgagcg tcggtccgcc gcaaggcccg tgatagcatc
      781 cgcgccgtcc cagaaatagt cgaagcacgc gcgctgaacc tgttccagca gcgcctcgtc
      841 ggccagcgcg cccagcgact gcggatcaga cgtcggcacg gacaggctcc ctgggtgcgg
      901 tgcgcttgcc gcgcgacttg ggttccgcgg gtgccgccgg acgtgacagt cgcggcgcgt
      961 ccggatcgtt caccagatgc agcacgcgcg cgtagggtgg accggcttgc tgtttgcgac
     1021 cgatatgaat cagtgcgctg tgcttgagct cgcgggcaaa cacatccttc acctgcgcca
     1081 gcgtgtgctc atcgagtgaa tcgaacacct catcgatcaa cacccacggc ggggctttca
     1141 gcagcagccg tgcaaacgcc agttgctgct gctcgtcctc gttgagctcc tgctcccagc
     1201 gcttggtctg atcgagcatc ggtgtcagcc gctgcagctg catgcgctcg agcgccttgg
     1261 cgtagctgtc ctggttgaac tcgtgcacct catagggata agcgagcacc tcgcgcaagc
     1321 tgccgggagg cagatacggc gtgcgtggca tatagcggat cgtctcctgc tcgggtcggg
     1381 taatacgacc gccaccccac ggccacagtc cggccaaggc gcggaacagc aacgtcttac
     1441 cggctccggt ctcgccgatg atcagcacgt gttcacctgc tttgagctcg ttatgaggct
     1501 ctttgagcat agtggcgccg ctgggggagg cgatttccag tcgatcgatc acaaatttcc
     1561 ccgctggtcc ttcagccagt tcaatctggc cggtcgcggc gtgcatctcg tggctgtcca
     1621 gcagcgcgcg gcgaaagctt gccacccgca gcagggtcgc gcgccagtca gcgagggtgc
     1681 tgaaattgtc gacaaaccag cgcaacgagg attgcacctg cgtgaatgcg ccggccgcca
     1741 tcatcagccc gccgaacgaa atcttcgagt cgaaataaat cggcgccgcc accaggatcg
     1801 gagccacgat cgtgaaccag ccgtagccgg aggtcaccca ggtcagattg gtcagtcccc
     1861 agaccagccg ccgcatcgct ttgagcaccg cattcagatc caggcgcaag cggcgcgctt
     1921 cgtcggtctc gccgccggcg agcgacaccg aatcgatatg ttcgctgact cgcaccagcg
     1981 agaagcgcag gtccgcttcg cgcgcgtagc gatccgcgtt ggcatcgatg aggttgcgac
     2041 ccacccagta actcaggagc gaggcggagc cggcgtagat gaacgctgcc cagaccatgt
     2101 agccggggat cacgagcagc cggccgcgga actgaaaccc gaagccgctg gagatgcccc
     2161 acagcacgct gatgaatgtg gccagcagga tcgaggcctg cagcaggccg atgcccaaat
     2221 ccgaggagag ctcggtcagg tgccgggcat cctcatgcat acgctgatcg ggattgaccc
     2281 ccatgggacc tgcgttcgcc agccgaaacg cgcgccgcgg caccagccac tcggcgatca
     2341 gatcgagcac caggccctga cgcagtttca gcttcagcat ttccccgagc catttgatgc
     2401 cgacattcaa tgccaatagc gagccggcga tgatcccaaa catgaccagc tggtacagga
     2461 atccggatag gtcgcggcgc gacagggtgt cgtagaaggg ctgattccag cgattgagcc
     2521 ggatctggcc ataggcagtc gcaatgacgt ccagcaggat gcacgcgccg agcaacaaca
     2581 gcgcattgcg taccggcgaa gcccacagcg cagcggtcat ctgacgcagc tgtggcagca
     2641 ggtgcgcctg cgcctgctcg ctgcgacggg tctggcgtgt gacttttctg gccatgtgac
     2701 ggtccttgcg aaacgatgcg ctaagggtat agaagatcgg atgcggagtg ggacggtatg
     2761 acccgaattg aatctctgat cagcgagatg acgctgacgg aaaagctcgg acagctgacg
     2821 atgaccgcct gcgggtacgc ggtcaccggg cctgtgatcg ccggggattc caccgaggcc
     2881 atcaaggacg gtgcgatcgg caatctgctc aacctggtgg gcgccgatca catccgggag
     2941 atgcaggatc tggccgtcaa acactcgcgg ctgaagattc cgctgctgat cgcgctggat
     3001 gtgatccacg ggttgcgcac gctgtttccg atcccgctgg cggaagcggc gacgttcgat
     3061 cgcgaaatct gggagcgcac ggcgcgcgcg gccgcgcagg aggccgccgc cgacggtatc
     3121 gcgatgacct tctcgccgat gctggacgtg gcacgcgatc cgcgctgggg acgcatcgcc
     3181 gaaggaccgg gtgaggatcc gtggctcgcg agccagattg cgctcgccaa ggtacgcggc
     3241 tatcagggca ccgatctggc agcggccagc gcgctagcgg cgtgcgccaa gcactattgc
     3301 gggtacgggg cggtcagcgc cggtcgcgac tatgcctcgg tcgatatctc cgagcgcacg
     3361 ctgcacgagg tgcacatgcc cgcctttcgc gcagcggtcg gagcgggtgt ggccagcgtc
     3421 atgcccgcgt ttacggatct ggccggtata ccgatgacgg tgcaccgtgc gctgctgcgg
     3481 gactggctgc gcggcagcta tggattcaat ggcgtgatcg tcagcgacta caactcgatt
     3541 ggcgagctgc tcaagcatgg agtcgcggcc gatctgaccg aagcggcgac actcgcactg
     3601 cgggcgggcg tggatatcga catgatgtcc aatgcctatc gcaccggctt gccactggcg
     3661 ctcgagcgcg gctgggtgaa catcaacgag atcgacgcgg cggtgcgccg cgtgctgacg
     3721 ctgaaggagc ggctcggtct attcgatgat ccgtaccgcc ggggttcggt gccggagaat
     3781 ctctcggtgc tcagtgaacg acgccaactg gcgcgccgtg cgagcgcgcg ctcgatcgtg
     3841 atgctgcaga atgtacgcgc gagcctgccg ctgccctcat cgctccgccg gctcgcgctg
     3901 ctcggtccgc tggcggatgc ggcggtggag atgggcggct gctggggtgc ggcctcgaaa
     3961 ccggacacgc atgtcagcgt gcggcaggga ctgcgcgcgg cactgcctga aaccgatatc
     4021 acctacgcgc ccggcgtcgc catcaatggc gatgaccaga gtcagatcgc agaggcgctc
     4081 gatctgtgcg accggaccga tgccattgtg ctgtgtctgg gcgagtcggc gaacatgagc
     4141 ggtgaggcgg ccagtcgtac cgatctggat ctgcccgggc tgcagcgcgg attcgcacag
     4201 gcagtattcg agagtgccag cacacacggc a
//
LOCUS       DATMRM010000125         3621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_57206, whole genome shotgun sequence.
ACCESSION   DATMRM010000125 DATMRM010000000
VERSION     DATMRM010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57206"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..702)
                     /locus_tag="VNZ06_03590"
     CDS             complement(64..702)
                     /locus_tag="VNZ06_03590"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HWW19865.1"
                     /translation="MYVPEHFRESRAQVLHRFIAQHPLATLVAPTRQGLIANHIPLLW
                     MAATEGPGTLRGHIARANGLWRAVEGSASVLAIFTGAQHYVSPTWYPSRRADGKAVPT
                     WNYAAVHVRGNIRFIEETGWLRGLVEALTEVHEGASAQRWHVDDAPASYIDTMLRSIV
                     GFEIDITGIEGKFKGSQNRSMEDRSSVAVRLREHGFSASQLAELVPGVEAKP"
     gene            complement(709..909)
                     /locus_tag="VNZ06_03595"
     CDS             complement(709..909)
                     /locus_tag="VNZ06_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19866.1"
                     /translation="MTDERLCASCRHFNRAAHRLEQELPGLRSLSSAHAAVRSDDGLC
                     SVHDRYVASYYTCPQYEAVRGE"
     gene            complement(906..2303)
                     /locus_tag="VNZ06_03600"
     CDS             complement(906..2303)
                     /locus_tag="VNZ06_03600"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HWW19867.1"
                     /translation="MSEIAHPTHAAAVARSSGWSDLIRQDDWWAIWIGLGLVAVAVAL
                     FANGDSIKWIAIAPQKWSHWQDLTAQLRAHGPQYGALFVLLEVLFGIGARALGYRLSQ
                     FLPSFLLVYAVSLAIYFVGQWDQASHYNLEPPLVALALGLLVSNTTGLPRWAQAGLRV
                     EFYIKTGIVLLGAGLPLTLIAWAGPVAVIQAGIISLVTFGTIFFAGRALGLDRRLAAV
                     LGTGGAVCGVSGSIAVAGAVGARKEQVSIGITLVVLWAIVMIFALPLVARGLHLATGV
                     AGAWIGTSEFADAAGLAAAQTYGGYAGNVPGIDGSANAAVNAFTLMKVIGRDIWIGVW
                     AFALSIIATTRWESTGVRGRANASEIWRRFPKFVLGFLIASAIVTLVVRGYDYASYKQ
                     TVLPGLVAPLQSFRTWAFTFAFLAIGLTTRVREFVAVGARPFLAFTLGVVVNVTLGFI
                     LSTQVFGDFWSHLGQ"
     gene            complement(2445..>3621)
                     /locus_tag="VNZ06_03605"
     CDS             complement(2445..>3621)
                     /locus_tag="VNZ06_03605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HWW19868.1"
                     /translation="TYWNALGLNTNPNRISIVNVDGGSGPLNGNSIETTVDVQQSGGI
                     APGANIIVYEAPNRSQAFLDAFVKAVSDNQADSVSTSFGSPEFLRDPNVTGITVTDPL
                     NGEQVSVLQAMHEQFVIAALQGQALSAAAGDSGAFDTVRQFGVGEQFTDPLSVDYPGS
                     DPALTSAGGTTLPGSLAFVGGGGTTVSINNPVERVWGWDYLEPVCNSFGLTDSQIDLF
                     NCGIFSVGTGGGVSVFFPIPFQQLAIFGTQLSQPAQSFTEIDVSPAVDIFDFPANFPG
                     RNVPDVSFNADPFTGYAVPFTGPTGLVVASLGGTSFVAPQLNGVSALLAQKAGHRIGL
                     LNVRLYNLQRLGGSFGPKPAIRTISTGDNWFFKGRNGYSPAVGVGTLDVTNLARLTF"
BASE COUNT          713 a         1227 c         1102 g          579 t
ORIGIN      
        1 gggggcactg ctctactggc gcggtgcgcg acgccggtcc gcaccggcca cccggcaagc
       61 ggcttagggt ttcgcctcca cccccggcac cagctccgcg agctgcgacg cggagaatcc
      121 atgctcgcgc agccgcaccg ccaccgagct gcgatcctcc atcgagcgat tctgactgcc
      181 cttgaacttg ccctcgatcc ccgtgatgtc aatctcgaac ccgacaatcg agcgcagcat
      241 cgtatcgata tagctcgccg gcgcgtcatc gacatgccag cgctgcgcgc tggcgccttc
      301 gtgcacctcg gtcagtgctt cgaccagacc gcgcagccag ccggtctcct cgatgaagcg
      361 gatattgccg cgcacatgca ccgccgcgta gttccaggtt ggcacggcct tgccgtctgc
      421 acgcctgctc ggataccacg tcggcgatac gtagtgctgc gcgccggtga aaatcgccag
      481 cacactggca ctgccctcta ccgctcgcca cagcccgttg gcgcgcgcga tatgtccacg
      541 cagagtgccg gggccctctg tggctgccat ccacagcagc gggatgtggt tggcgatcag
      601 gccctgtcga gtcggcgcga ccagtgtagc gagcgggtgc tgggcgatga atcgatgcag
      661 cacctgtgcg cgcgactcac gaaaatgctc cggaacgtac atcgcctctc actcgccgcg
      721 gacggcctca tactgcgggc acgtataata ggacgcgacg tagcgatcgt gcacgctgca
      781 cagaccgtca tccgaacgca cggcggcgtg tgccgaactc aagctacgca gtcccggcag
      841 ctcctgctcc agacggtgcg ctgcgcgatt gaagtgccga cagctcgcgc acagccgttc
      901 gtcagtcact gacccagatg gctccagaag tcaccgaaca cctgcgtcga gagaatgaaa
      961 cccagcgtca cgttgaccac cacgccgagc gtgaatgcga gaaacggacg tgcgcccacg
     1021 gcgacgaact cacgtacccg cgtggtcagt ccgatcgcga ggaacgcaaa cgtgaacgcc
     1081 caggtgcgaa acgattgcag cggcgccacc agtcccggca gcacggtctg cttgtagctg
     1141 gcgtagtcgt agccgcgcac caccagcgtg acaatggcgg acgcgatcag aaacccgagc
     1201 acgaacttcg gaaaacgccg ccagatctcg ctcgcattgg cacgtccgcg cacgccggtt
     1261 gattcccagc gcgtggtggc aatgatcgac agcgcgaatg cccagacacc gatccagatg
     1321 tcgcggccga tgactttcat cagcgtgaag gcgttcaccg cggcgttggc gctgccatcg
     1381 atgccgggta cgttgccggc gtaaccgcca taggtctgcg cggccgccag cccggcggca
     1441 tccgcgaact cggacgtgcc gatccaggcc cccgcgacac cagtggccag atgcagtccg
     1501 cgcgccacca gcggcagcgc gaagatcatc acgatagccc acagcaccac cagggtgatc
     1561 ccgatcgaga cctgctcctt cctggccccg acggcgcccg cgaccgcgat cgatccggag
     1621 acaccacaca ccgcaccgcc ggtgcccaga accgccgcca gcctgcgatc cagtcccagc
     1681 gcgcgcccgg caaagaagat cgttccgaag gtcaccagcg aaataatccc agcctgtatc
     1741 acggccacgg ggcctgccca ggcaatcagg gtgagcggca gtccggcacc gagcagcacg
     1801 atgccggtct tgatgtagaa ctcgacccgc agtccggcct gcgcccagcg cggcagtccc
     1861 gtggtgttgg acaccagcag tcccagcgcc agcgccacca gcggcggctc gaggttgtaa
     1921 tgcgaggcct ggtcccactg accgacgaaa taaatcgcca gcgagaccgc atacaccagc
     1981 aggaacgacg gcagaaactg actcaggcgg tagccgagcg ccctggcgcc gattccgaac
     2041 agcacctcca gcagcacgaa cagtgcgccg tactgtggcc cgtgagcgcg cagctgcgcg
     2101 gtcagatcct gccaatggct ccacttctgc ggggcaatcg cgatccattt gatgctgtcg
     2161 ccgttggcaa acagtgccac ggcgaccgcg actaacccca ggccaatcca gatggcccac
     2221 cagtcatcct gccggatcag atcagaccag ccggagctgc gtgccacggc agcggcgtgt
     2281 gtcgggtgcg cgatctcgga catggaactc ctccggcctg aagcctccct gagcctagag
     2341 gagtccctat ccggaatcga ttagcgatct gtcatgtcga attgcgcgtc ttgtcatgtt
     2401 accgcgccgt gacctgaccg tcgccgcggc acctattgca gcgctcagaa tgtcagacgc
     2461 gccagattgg tgacgtcgag cgttccgact cccacggcag ggctgtagcc gttgcggcct
     2521 ttgaagaacc agttgtcgcc ggtggagatc gtgcgaatgg ctggcttggg tccaaacgat
     2581 ccgcccagac gttgcaggtt atacagccgc acgttcagca ggccgatgcg gtgcccggcc
     2641 ttctgtgcca acagcgcgct cacgccgttc aattgcgggg cgacgaagct ggtaccgccc
     2701 agtgaggcga ccacgagccc tgttggaccg gtgaacggca ccgcataacc ggtgaacgga
     2761 tccgcattga aggagacgtc cggcacgttg cgccccggga agttcgccgg gaaatcgaag
     2821 atgtccaccg ccgggctgac atcgatctca gtgaatgact gcgccggctg actcagctgc
     2881 gtaccaaaga tcgccagctg ctggaacgga atcggaaaga acacgctgac gccgccgccg
     2941 gtaccgaccg aaaaaatccc gcagttgaat agatcgatct ggctgtcggt caggccgaat
     3001 gaattgcaaa ccggctccag atagtcccag ccccagacac gctcgaccgg attgttgatg
     3061 gagacggtgg tgccgcctcc gcccacaaac gccagcgagc ccggcagcgt ggtaccaccg
     3121 gccgaagtga gcgccgggtc gctacccggg tagtccacac tcagcggatc ggtgaattgc
     3181 tcgccaacgc cgaattgccg aaccgtgtcg aacgcgccgc tgtcaccggc ggcggccgac
     3241 agggcctgtc cctgcaacgc cgcgatcacg aactgctcgt gcatcgcctg cagcacgctg
     3301 acctgttcgc cgttgagagg gtcagtgacc gtgataccgg tgacgttcgg atcgcgcaga
     3361 aactccggcg aaccgaagct ggtggacacg gaatcggcct ggttgtccga caccgctttg
     3421 acaaacgcat cgaggaacgc ctggcttcgg ttcggtgcct catagacgat gatattggcc
     3481 ccgggcgcga tgccaccgga ttgctgcaca tctaccgtcg tctcgatcga attgccattc
     3541 agcggccccg agccgccatc cacattgacg atcgagatgc gattgggatt ggtgttgagg
     3601 ccgagcgcgt tccaataggt g
//
LOCUS       DATMRM010000126         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2549350, whole genome shotgun sequence.
ACCESSION   DATMRM010000126 DATMRM010000000
VERSION     DATMRM010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2549350"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2036)
                     /locus_tag="VNZ06_03610"
     CDS             complement(<1..2036)
                     /locus_tag="VNZ06_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HWW19869.1"
                     /translation="MRYSLTVSRGLREGGAIVLAALALVVVVALLTFDPGDHSLWSWS
                     SVSNAPVHNRVGFIGASLASGLYALFGRPAYLLPLMLGISAWRMFRARAEGHKQYSRA
                     NVTIRVAGFVIVLIASCALAALHWSPGALPQSAGGVVGTATGHELESGLNILGATLIL
                     LAVWMAGVAIAFGISWLTLMDHVGAGTWATVHWVRNRWFNRQAEEAAQAGRSARLERQ
                     EALQAERQRAPTRMAPRIIEPPAPTPRKSERAEKERQVPLFDPPQAKELPPLGLLDDP
                     GEREPSYSSEALEAMSRLVELKLKDFGVDVEVVAVHPGPVVTRFEMRPAPGVKASQIT
                     ALSKDLARALSTISVRVVEVIPGKSVMGLEIPNEKRELVTLGEIIKSKAYDDIVSPLT
                     LALGKDIGGVPMVADLARMPHLLIAGTTGSGKSVGINAMVLSLLYKATAEHVRLIMID
                     PKMLELSVYEGIPHLLSPVVTDMKQAANALRWCVAEMERRYKLMAALGVRNIAGYNRR
                     VKDSIEDGQPILDPVLMQAASNDPSIDQSQIPNLEPLPFVVVIIDELADLMMLVGKKV
                     EELIARLAQKARASGIHLILATQRPSVDVITGLIKANIPTRIAFQVSAKVDSRTILDQ
                     MGAESLLGHGDMLYLPPGTATPTRIHGAFVSDQEVHRVVDALKSAAAPNYIEDVL"
     gene            2151..>2365
                     /locus_tag="VNZ06_03615"
     CDS             2151..>2365
                     /locus_tag="VNZ06_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020796668.1"
                     /note="catalyzes the transfer of electrons from NADPH to
                     thioredoxin; FAD/NAD(P) binding; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HWW19870.1"
                     /translation="MTAPRHRRLIILGSGPAGYAAAVYAARANRSPLLITGLEQGGQL
                     MTTTDVDNWPGDVEGLQGPALMERMKR"
BASE COUNT          445 a          789 c          711 g          420 t
ORIGIN      
        1 agcacgtctt cgatgtaatt cggggccgcc gcgctcttga gcgcatcgac cacacggtgc
       61 acttcctgat cggagacgaa cgcgccgtgg atgcgcgtcg gtgtcgccgt gcccggcggc
      121 aggtacagca tgtctccatg accgagcagc gattcggcgc ccatctgatc cagaatcgtg
      181 cgcgaatcca ccttagccga gacctgaaag gcgatgcggg tcggaatatt ggccttgatg
      241 agaccggtga tgacatccac cgacggccgt tgcgtggcca gaatcaaatg aatgccggag
      301 gcgcgcgcct tctgagccag tcgcgcgatc agctcctcca ccttcttgcc gaccagcatc
      361 atcaggtcag cgagttcgtc gatgatcacc accacgaacg gcagcggctc gagattcggg
      421 atctgactct gatcgatgct cgggtcgttc gaagccgcct gcatcagcac cggatcgagg
      481 atgggctggc catcttcgat cgagtccttc acgcggcggt tgtagccggc gatgttgcgc
      541 acacccagcg cggccatcag tttgtagcgc cgttccatct cggcaacaca ccagcgcaac
      601 gcgttcgccg cctgcttcat gtcagtcacg accggtgaga gcagatgcgg aatgccctca
      661 tacaccgaca gttccagcat cttcggatcg atcatgatca gacgcacgtg ctcggcggtg
      721 gccttgtaga gcaggcttaa gaccatggca ttgatgccga cggatttacc cgaaccggtg
      781 gtgccagcga tcagcaggtg cggcatacgc gccagatccg caaccatcgg cacaccgccg
      841 atgtccttgc ccaacgccag cgtcagcggc gagacgatgt cgtcgtaggc cttggacttg
      901 atgatctctc cgagcgtcac cagctcgcgt ttctcgttcg ggatctccag gcccatcacg
      961 gattttcccg ggatcacttc caccacgcgc acgctgatcg tggacagcgc gcgcgccaga
     1021 tccttcgaca gcgcagtgat ctgactggcc tttactcccg gcgccggccg catctcaaag
     1081 cgcgtcacga ccggaccggg gtgcaccgct accacttcga catccacgcc gaagtccttg
     1141 agcttgagct ccaccagtcg cgacatcgct tccagcgctt ccgacgaata cgacggctcg
     1201 cgctcgcccg gatcatccag cagacccagc ggtggcagct ctttggcctg cggtggatca
     1261 aacagcggta cctgtcgctc tttctccgcg cgctcgctct tgcgcggcgt tggcgctggc
     1321 ggctcaatga tgcgcggtgc cattcgggtc ggcgcacgct gccgttcggc ctgcagggcc
     1381 tcctgacgct cgagtctggc cgagcgtcca gcctgcgcgg cctcttcggc ttgccggttg
     1441 aaccagcgat tgcgcaccca gtgcacggtc gcccaggtac ctgcgccaac gtggtccatg
     1501 agcgttagcc atgagatgcc aaatgcgatc gccacgccgg ccatccacac ggcaagcagt
     1561 atcagggtgg ccccgaggat gttgaggccg gactcgagtt catgcccagt ggccgtgccc
     1621 accacgcctc cggcactttg aggcaatgcg cccgggctcc agtgcagcgc cgccagcgcg
     1681 caactggcaa tcaatacgat cacgaacccg gcgacgcgga tcgtcacgtt ggcgcgcgag
     1741 tactgtttgt ggccctcggc gcgcgcgcga aacatccgcc aggcggaaat ccccaacatg
     1801 agcggcagca ggtaggcagg gcgcccgaac agcgcgtaca gcccactggc cagcgaggcg
     1861 ccgatgaagc cgacacggtt gtgcaccggt gcattgctga ccgaagacca tgaccacagg
     1921 ctgtgatcgc ctggatcgaa cgtcagcagg gccaccacca ccaccagcgc gagcgctgcc
     1981 agcacgatcg ccccgccctc gcgtaacccg cgcgagaccg tcagcgaata acgcatggga
     2041 gcacgttgtg cagtggactc ggtcacgaaa ttgcaaacct cattgaagga tatgacttgg
     2101 cctgcggacc gcgcttactt accatccatg attccaacag gaaattgtac atgactgcac
     2161 ccaggcaccg ccgattgatc attttgggct ccggtcccgc cggttacgct gccgcggtct
     2221 acgcagcgcg ggccaaccgc tcccccttgc tgatcaccgg tttggagcag ggcggacagt
     2281 tgatgaccac gaccgacgtg gacaactggc ccggggatgt cgaagggctg caggggccgg
     2341 cgctcatgga gcgcatgaag cgcca
//
LOCUS       DATMRM010000127         9207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3466317, whole genome shotgun sequence.
ACCESSION   DATMRM010000127 DATMRM010000000
VERSION     DATMRM010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9207
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3466317"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VNZ06_03620"
     CDS             <1..684
                     /locus_tag="VNZ06_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl reductase"
                     /protein_id="HWW19871.1"
                     /translation="SAAELLDPVGMANYVAANSFLEGLARRRRALGLRATCVAWGGWS
                     DLGMAGKMSQSWERRWERRGIQGLDTYRSFDLLLRLIDAGPSVALVMQLDWHRFAATA
                     SADQRALFAPMLPKEESLSSKKVHSISVASLPAYPPRPLDGESDIASIVHRRALNILQ
                     LPEQSRLDPDMPLADLGFDSLMSLELRDVLATDLNTALPATLLFDHPTLSSLIPFVSA
                     QVRDRYATS"
     gene            720..1142
                     /locus_tag="VNZ06_03625"
     CDS             720..1142
                     /locus_tag="VNZ06_03625"
                     /inference="COORDINATES: protein motif:HMM:NF023123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HWW19872.1"
                     /translation="MPDVPTGDRMPDDGLFVAQLNVAQALYELDDPRMASYLSLIEEV
                     NAAADRAAGFVWRLKDGSGKLNVGRSSREIVNLSVWRSLDDLRDFVHGEIHGHALRKR
                     SKWFIRLPSPYSVVWSIPLGRLPTLQEGLKRLAAPQHE"
     gene            complement(1111..2319)
                     /locus_tag="VNZ06_03630"
     CDS             complement(1111..2319)
                     /locus_tag="VNZ06_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWW19873.1"
                     /translation="MNGVDSLRQHRSSYPALSERTYLNFGARGVMPRVAREAILNSLD
                     TLQSVGPASRAGIAWIEYELFATRRSLAALIGAPPERIALLDSVSNACSLVIFGIRWA
                     ASDHAVVCEFEPPGTWLAIEQASRRFGFEVSGFPAPTGPNDTQWREALLRALRPSTRL
                     VIASHINWITGAHLAPSQLVSVVRASSAPRALILVDGAQAIGVLPFELRSSEVDLYAF
                     GGQKWLGGPDGIAALFVSESAQNAVEPSIVGWRGLNPAPEIDRLSLSSTAVRYEGGTA
                     AYCLLAGLRAAVEAQQSFATLAERFASIEALTARFRRQIAQMNADATRPIQILGEDAR
                     SGITTFQIEGTCPSAVYRRLEGAGMVIKQHARPPSVRVCAHYLTLTEEIDQLCGQLRH
                     FTHAGARPGA"
     gene            complement(2337..8609)
                     /locus_tag="VNZ06_03635"
     CDS             complement(2337..8609)
                     /locus_tag="VNZ06_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWW19874.1"
                     /translation="MKTQPESGRDLIHAAARASERDIAVVGMACRFPQSVNPQSFWNA
                     LISKADAISEIPRERWDADAFYDPTPGTPGKMYSRRGAFLRDIDLFDEKFFRVTPREA
                     KSMDPQQRLLLEVSWEALESAGQVHRNLERSATGVFIGISSSEYAMLAGNSGKLDEID
                     KYFGTGTALSVAAGRISYFLNLRGPSMAIETACSSSLVAVHQAVRSLRSGETDLCIAG
                     GVNVILSPAVGVYFSQLGAVSASSRCKAFDADADGYVRGEGCGIVILKRHKDAERDDD
                     NIIALIRGSAVNHDGRSAGLAAPNGAAQEEVLKAALRDGDVRPEDVAYIEAHGTGTRL
                     GDPIEAKAIGRAYAAADARRNPLLLGSVKTNMGHLEAAAGVAGLIKVCLALRERCIPP
                     QINFQTRNPLIPWDELNLAVASECVSWPADAEPLAAVSSFGFSGTNAHIVLSAAPAGA
                     SSAPTPTGSTYLLPVSARSETALSALLRRCDERLAQTPQDSSDSVLAMCRGWALRREH
                     YAHRRCVVGRDAAELRRSMVNSPAVDGGALSPDAAVPARVAFIFSGQGSQWQGMAKEW
                     LRQDTTTAVLLEIDAIVRRKTGWSIVEELSAETPRAQVDVTQVCLFALQVALARRWQQ
                     VGLTPNVVVGHSMGEIAAAHIAGALNLEQATELSIVRSQLLASVGGQGQMAVADLPRE
                     QAVELARHWQGRVFVAGQNSFSSTIFAGIAEDIEDLIAHLSAESVFCRKLRTEGVASH
                     CPLVASLGKRINERFADLRPQPSHLALVSSVTGLPLSGEQLNAGYWRRNLTEPVRFAD
                     AVGHIASTGNEIFVEISPKPILLSSVEQVCQALGKRAIVVPSMRQSEGAVETFSRSLA
                     TLYESGVEIDWKAIYPGRGRWEPATTYPWQRQRHWITEIVASETKSPLGTSSTQLPES
                     IWRWDRFGMDSVNMGLLAPWILLSPDRSSAFYLGLSGDIVIAWAYLGDPLDFEHQVSQ
                     LASTCERHSLQLALMADESAAQSMQRLSLRSIPVGSMHRLTDLRHFTLSGQGMRRLRN
                     QVQRFERETKGTLAEYTPGSDRSMDAAIVALIGHWQQRVGEPVPAATWIQESLLSGRG
                     LGKRRLFVTCAGDRVVSAVLLTPFGNRAGYLLDMEFYGQKVPPGCNEFAIVRAAQLLA
                     AEGFQELSLGGSYGIEASARSQSAPDRSPRGYAGQRQFKRKFTANEEGIYLYTTQDRA
                     TDAAAAFFALLADRDVSGPLQQPRVETSLEAVLGARPSHPFLKHTPRVSADSVSFEST
                     IRSGDPYIGQHCIDQTPVMPGALYIDLMLTAISRARPGTSWALTDLVLLEPLLFGDDI
                     AHRVRIDVLASTATAELVIWSKSSVDPTTPWRRHVSSRATHSSTPPCASPESASGNER
                     WRVIDPEVLYAGLRAAGIEHGPMYRAVRELRLAGTTLEAQLQLPSAAAGDKDGFTLNP
                     VVVDAALQVLAAFAEPGALQPVVLQAVQRICVYRKPTRQCRVRVLARNDDAPGLYSSD
                     VLITTLAGERIATLEGVIARVIRPARSKARSSFHHALYTYELAERALPLARGFDGTCI
                     LIGPRSELFDRSAESIAALGGRIKAFTASTRVEAFAELKSLPDSEWSDVSQLLMLPER
                     TPTNGSLPASAYGAFLPDLVRLLGTRSTRAGFRVSLVTNGPDGTQDEQASTADAALGA
                     ACGVLQAEHPEYAVTRIELNTRVAAERAARAIAAICSNSADREWWSIGSYTCGVFRIA
                     ATVVPPKASWTPRADTTHIVTGWTGGIGTRVVHWLLERGVRALALISRSRGPISVNQE
                     IERATAAAGARIHRFEADIADWQAMQGIFAHLAQHAPPIETIFHVAGQTADTLLFKQM
                     PDEFMTAQHAKIAGTWNLHRASASLSVRAFVMFSSAASLFCPPSQAAHTAASAAMDQI
                     ARLRRCAGLPAMSIGWGPWSQIGKAAQMGIGERLSSMGIDPISTDDALEGLDQLMVCD
                     LPQVFLVPVDWNRWRASGGPVAQLPLFSGLATTPEPAAEKGAKVALRDTPATHTVDSV
                     QARIVRCAAQTLMMQPQEIDLGRSLEGIGMDSIMVVELRERLSRELGQSLPLIEFFRA
                     ASIASLAPRVFGHLQV"
     gene            8743..>9207
                     /locus_tag="VNZ06_03640"
     CDS             8743..>9207
                     /locus_tag="VNZ06_03640"
                     /inference="COORDINATES: protein motif:HMM:NF019624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1702 family protein"
                     /protein_id="HWW19875.1"
                     /translation="MLQRVVSAMRAQLFGISGDQALFRRRGFKSARAKSQHVLEDIGK
                     TFIDGYNFAVRSADPTLQSARLDSIPVDMHGFFVEGASMGFALFDIVSPWPTRFFQEF
                     IRQAASPHIYMAYVGAGWALARTSLRLSWRLGKTDPLLRWLMFDGLGFHHGYF"
BASE COUNT         1725 a         2821 c         2839 g         1822 t
ORIGIN      
        1 tcagcggcgg agctactgga tcccgtagga atggcgaact atgtggctgc caactcattc
       61 ctggaaggtc tcgcgaggcg acgcagagct cttgggctcc gcgcgacgtg tgtcgcctgg
      121 ggcgggtggt cggatttggg catggcaggg aagatgtctc aatcatggga gcggcgctgg
      181 gagaggcggg gtatacaggg ccttgatact taccgatcct tcgaccttct gttgcggcta
      241 attgacgccg gaccctccgt cgctctggtg atgcagctgg actggcatcg atttgcagct
      301 acggccagcg ccgaccaacg cgcgctgttc gccccgatgc tgccaaaaga ggagagctta
      361 tcttccaaga aggtgcactc tatttcggtg gcttcgctcc cggcgtaccc gccgagacct
      421 cttgatggtg aatctgacat cgcctccatc gtccatcggc gcgcgcttaa cattctgcag
      481 ctgcccgagc agtccaggct cgatccagat atgccgctgg cggatcttgg atttgactca
      541 ttgatgtcgc ttgagcttcg cgatgtgctt gccacggatc tcaataccgc tcttccagcg
      601 acactgctgt tcgatcaccc aacgttgagt tctctgatac cgttcgtttc ggcgcaggtg
      661 cgagaccggt atgcgacctc ttgagccaat gccaatggcc gccacctgta gcagtagcgg
      721 tgcccgatgt gccaacgggt gatcgcatgc ccgacgatgg attgttcgtc gcccagttga
      781 acgtcgctca ggctttgtac gagctggatg atccgcgtat ggccagctat ctgtcgctga
      841 tagaggaggt caacgcagcc gccgatcgcg cggcggggtt tgtctggcgc ctgaaggacg
      901 gctcgggaaa gttgaacgtc gggaggagct cgagagagat cgtcaatctg tcggtgtggc
      961 gctccctgga tgacctgaga gattttgtcc acggggagat ccatggtcat gccctacgca
     1021 agcgttccaa gtggttcata cggctgccct cgccgtattc ggtagtgtgg tcaataccgc
     1081 tggggcgtct tccgaccctt caggagggac tcaagcgcct ggccgcgccc cagcatgagt
     1141 gaaatgtcgc agctgcccgc agagttggtc gatctcctca gtcagagtca gatagtgggc
     1201 acatactctg acgctggggg gcctcgcgtg ctgtttgatc accatgccgg cgccctcgag
     1261 acgccggtaa acggctgacg gacacgtccc ctcaatctga aacgtggtga ttccggatcg
     1321 tgcgtcctca cccagtatct gaatcggtcg cgtggcgtcg gcgttcatct gcgcgatctg
     1381 ccggcgaaac cgggctgtca gtgcctcgat gctcgcgaat ctttcggcca gggtggcgaa
     1441 ggactgttgg gcctccacgg ctgctcgcaa ccccgcgagc aaacagtaag cagctgttcc
     1501 tccttcgtag cgcacggcgg tagatgagag gctgagtcgg tcgatctcgg gggcggggtt
     1561 caggccgcgc catcctacga tgctgggctc gaccgcgttc tgtgcgctct cgctgacgaa
     1621 cagtgcagca atgccgtcag gaccgcccag ccatttctga cctccgaatg catagagatc
     1681 gacctcagag gagcgcagct cgaaggggag gaccccgatg gcctgagcgc catccaccag
     1741 aattaacgca cggggagcag agctggctcg taccacactg accagctgag atggcgcaag
     1801 gtgcgcgcct gtgatccagt tgatgtgact tgcgatcacc agacgtgtgc tcggacgcag
     1861 agcgcgcagt agcgcctccc gccactgagt gtcgttcgga cccgtgggag ctggaaagcc
     1921 ggacacctca aatccaaatc ttctggaggc ctgctcgatc gcaagccatg ttccgggcgg
     1981 ctcgaattcg cacaccaccg cgtgatccga cgcggcccat cgaattccga aaatcaccag
     2041 cgagcaggca ttggagacac tatcgagcaa cgcgatccgc tcgggcggtg cgccgatcaa
     2101 cgctgcgagt gagcgtcgcg tcgcaaacag ctcgtactcg atccaggcga tgccggcgcg
     2161 cgaggcgggc ccgaccgact gcagtgtatc caggctgttg agtatcgcct cgcgggcgac
     2221 acgcggcatg acgccgcgcg ctccgaagtt caggtatgta cgctcgctga gtgccggata
     2281 gctgctgcgg tgttgtcgca gagagtcaac gccattcatg cgtcgagagc caggcctcaa
     2341 acctggagat gtccaaacac ccttggagca agcgacgcga tgctggcggc gcgaaagaat
     2401 tcgatcagcg gtaacgattg tccaagctca cgagacagac gctcccgcag ctcgaccacc
     2461 atgattgagt ccatgccgat tccctccagc gagcggccca ggtcgatctc ctgcggctgc
     2521 atcatcagcg tctgagcagc acatcgcacg atccgcgcct gtaccgagtc taccgtatgc
     2581 gtcgcgggtg tatcgcgcaa tgcgaccttc gctccttttt ccgcagcggg ctcgggagtc
     2641 gtcgctagcc ctgaaaacag tggcagctgt gccacgggcc ctccgctcgc gcgccagcgg
     2701 ttccaatcga ccggcaccag aaatacttgt ggcagatcgc agaccatgag ctggtcaagt
     2761 ccttccagcg catcgtcggt ggagatcgga tcgatcccca tagaactgag acgctcgccg
     2821 attcccatct gtgcggcctt accaatctgc gaccaggggc cccagccaat actcatcgcc
     2881 ggcaatccag cgcagcgacg cagtcgcgca atctgatcca tcgcggcact tgcggccgtg
     2941 tgcgccgctt gggatggggg acaaaagaga ctggcagcag aggaaaacat cacgaaggct
     3001 cgcaccgaca gagaagcact ggcgcgatgc agattccacg tgccggcgat cttggcgtgc
     3061 tgcgctgtca tgaactcgtc gggcatttgc ttgaacagca gggtatccgc agtctgaccg
     3121 gccacatgaa atattgtctc gataggaggg gcatgttgag ccagatgcgc gaagatgcct
     3181 tgcatggcct gccagtcggc tatgtcagcc tcaaatcgat ggatgcgcgc accggctgcg
     3241 gctgtcgcgc gctcgatctc ctgattgacg ctgatagggc ctcgacttct ggaaatgaga
     3301 gcgagcgccc ggactccgcg ctccaacagc cagtggacca cgcgggtgcc gatgcctccg
     3361 gtccatccgg tgacgatgtg tgtcgtgtct gctcgagggg tccaggaggc cttaggcggt
     3421 acgacggtcg cagctatcct gaacacaccg caggtgtacg aaccaattga ccaccattcg
     3481 cggtccgcgg aattggagca gatggcagca attgccctcg cggcacgctc ggcagccacg
     3541 cgggtgttga gttcgatgcg agttaccgcg tactcggggt gttctgcctg aagtacgccg
     3601 caggccgcgc ccagcgctgc gtctgcagtc gatgcttgct catcctgtgt tccgtcaggg
     3661 ccattggtca cgagtgacac cctgaaccct gcgcgcgttg accgcgtgcc gagcagacgc
     3721 accagatcgg gaaggaaggc accgtaggca gacgcgggca gtgagccgtt ggtgggcgtg
     3781 cgttcgggta gcatcagcag ctgtgatacg tctgaccact ctgaatcggg caagctcttc
     3841 agttccgcga acgcctcgac tcgagtggag gccgtaaacg ctttgattct gccgcccagt
     3901 gccgcaatgc tttctgcgga ccggtcgaac aactccgatc gggggccgat caggatgcag
     3961 gtaccgtcga atccacgagc caggggaagg gctcgttcgg caagttcata cgtgtacagc
     4021 gcgtgatgaa agctcgaccg ggctttactg cgcgccggcc tgatgacacg agcgatcacc
     4081 ccttcgagag tggcgatacg ctctcccgcc aaggtcgtga tcagcacgtc gctcgagtac
     4141 agcccgggag catcgtcgtt tctggcgagc acgcgtaccc gacactgacg tgtgggcttg
     4201 cggtaaacgc agatgcgctg cacggcctgt agcactacgg gttgcagcgc gcctggttcg
     4261 gcgaaggccg caagcacctg cagggcagca tcgacgacca ctgggttcaa cgtaaagcca
     4321 tctttgtctc cagccgcagc actcggcagc tgcagctgtg cctccagtgt cgtgccggca
     4381 agtcgaagtt cccggactgc tcggtacatg ggtccatgtt cgatgcccgc tgcgcgcagg
     4441 cccgcgtaca acacctcggg atcgatcaca cgccatcgct cgttccccga tgcgctctcc
     4501 ggcgaggcgc aaggaggtgt gctgctgtgc gtcgcccgac tgctgacgtg acgacgccag
     4561 ggtgttgtcg gatcgacact actctttgac cagatcacca gctctgctgt tgccgtcgag
     4621 gcgagcacat cgatacggac gcgatgggca atgtcgtcgc caaacagcag cggctcgagc
     4681 agcactaggt ccgtcaaagc ccaggatgtg ccggggcgcg cacgagaaat cgcggtcagc
     4741 atcaggtcaa tatagagagc acctggcatg accggtgttt gatcgatgca atgctgaccg
     4801 atataggggt cgccggatcg aatggtggat tcgaatgaca ctgagtctgc actgactcgc
     4861 ggtgtgtgct tcaggaatgg atgcgagggc cgcgcaccga gcaccgcctc cagtgaggtt
     4921 tcaacgcgcg gttgctgcag cggcccgctc acgtcgcggt cagccaacaa tgcaaagaac
     4981 gctgcggccg cgtcagtggc gcgatcctgc gtggtgtaca aatatatgcc ctcttcgttg
     5041 gcggtgaact ttcgcttgaa ttggcgctgc cctgcatagc ctctaggtga ccgatctggc
     5101 gcgctttgcg acctggcgct ggcttcgatc ccatagctgc cgccgaggct cagttcctgg
     5161 aagccttccg cagccagcag ttgagcggcg cgtacgatgg cgaattcatt gcaaccaggc
     5221 ggtaccttct gtccatagaa ctccatgtcc agcagatatc cggctcgatt gccaaatggt
     5281 gtaagcagca cggcgctcac tacccgatcg ccggcacacg tgacgaataa acgacgcttg
     5341 ccgagtccac gtccagaaag cagcgactct tggatccagg tcgccgccgg cactggctcg
     5401 ccaacgcgct gctgccagtg gcctatcagc gctacgattg cggcgtccat cgagcgatcg
     5461 gagccaggcg tgtactcggc gagggtccct ttggtctctc gctcgaagcg ttgtacctga
     5521 tttctgaggc ggcgcattcc ttgcccgctg agggtgaagt gcctcagatc ggtcaggcgg
     5581 tgcatcgagc ctacgggaat gcttctgagg ctcagccgtt gcatggactg cgccgcagat
     5641 tcatcggcca tcaatgccag ctgcaacgag tggcgctcgc atgtgctcgc gagctgggat
     5701 acctgatgct cgaagtccaa ggggtcacct agataggccc atgcgatgac gatgtcaccg
     5761 ctcaatccca ggtagaacgc ggaacttcga tccgggctca gaagaatcca tggggcaagt
     5821 aggcccatgt tcacggaatc catgccaaac cggtcccagc gccatatgga ctcggggagc
     5881 tgcgttgacg acgtcccgag aggggacttc gtctcggacg ccacgatctc agtgatccag
     5941 tggcgctgtc gctgccaggg ataggtggtc gctggctccc aacggccacg tcccggatag
     6001 atcgccttcc agtcgatctc gacgccggac tcgtacaatg tggcgaggct gcggctgaaa
     6061 gtctcaaccg cgccttcgct ctgccgcata gagggcacaa cgatggcgcg cttaccaagc
     6121 gcctggcaca cctgttcgac cgatgacagc agtatcggct ttgggctgat ttcaacaaaa
     6181 atctcgttgc cggtgctcgc aatgtggccg acggcgtccg cgaagcggac aggctctgtg
     6241 agattgcgcc gccaatagcc cgcgttgagt tgttcgcccg atagaggcag acctgtgacg
     6301 ctcgagacga gcgcgaggtg ggagggctgg gggcgcagat cggcgaagcg ctcgttgatg
     6361 cgcttcccca acgaagcaac gagcgggcaa tgactggcta ccccttcggt gcgcagcttg
     6421 cggcaaaaca ccgattccgc actcaagtga gcgatcagat cttcgatgtc ttccgcaatg
     6481 ccagcgaaaa tggtggaact gaagctgttc tggcccgcga caaatacgcg cccctgccag
     6541 tgccgcgcaa gttcgacagc ctgctcacgc ggcaggtcgg cgacggccat ttgtccctgt
     6601 ccgccgacgc tggcaagcag ctgactgcgc acgatgctca gctccgttgc ctgttcgaga
     6661 ttcaacgcgc ctgcgatgtg cgcggctgca atttctccca tgctgtgccc gacgactacg
     6721 ttgggtgtga gcccgacttg ctgccatcgt cgcgccagtg ctacctgcag agcgaacagg
     6781 cagacctgtg tgacatcaac ctgggcccgc ggtgtctctg cgctcaactc ctcgactatc
     6841 gaccagccgg tcttccgacg cacgatcgcg tcgatctcta gtagcaccgc ggtagttgtg
     6901 tcctggcgaa gccattcttt ggccatgcct tgccactgcg atccttgccc ggagaaaatg
     6961 aaggctaccc gcgccggcac cgccgcatcg ggcgacaacg ctccgccgtc aacggccggt
     7021 gagttgacca ttgagcgccg caattctgcg gcgtctcggc caaccacaca tcttctatgc
     7081 gcatagtgct cccgtcttag cgcccatccg cggcacatag ccagcacgga atcggaacta
     7141 tcctgaggag tctgggcgag acgctcgtcg caccggcgta gcagcgcact gagtgctgtt
     7201 tcagaccgcg ccgacaccgg cagcaggtag gtgctgcccg tcggtgtcgg tgctgatgac
     7261 gcgcccgcag gcgcagcgga aaggacgatg tgcgcgttag tcccactgaa tccaaatgag
     7321 ctcacggccg ccagcggctc tgcgtcggca ggccagctga cgcattcaga cgccactgcg
     7381 aggttgagct catcccaggg gatgagtgga ttgcgcgtct gaaaattgat ctgcggcggt
     7441 atgcagcgct ccctcagtgc cagacacacc ttgatgagac cggcgactcc cgcggccgct
     7501 tccaggtgcc ccatattcgt cttgaccgaa ccaaggagca gcggattccg acgagcgtct
     7561 gcggctgcat aggcgcgacc gatggctttg gcttcgatcg gatcgccgag gcgcgtaccg
     7621 gtcccgtgtg cctcgatata ggcaacgtct tctggtcgaa cgtcgccgtc gcgcagcgcc
     7681 gccttcagta cctcttcctg tgctgcgcca ttcggtgccg cgagtcctgc gcttctgccg
     7741 tcgtgattga ccgctgaccc gcgaatgagg gcaatgatgt tgtcatcatc gcgctccgca
     7801 tccttatgtc gcttcaggat tactattccg cagccttcgc cccgcacgta gccatcggcg
     7861 tcggcgtcaa aggccttgca gcgtgagctg gcggagaccg ctccaagctg tgaaaaatag
     7921 accccgacgg ccgggctcaa aatgacattt accccgccgg cgatgcacag gtcagtctcg
     7981 ccgctacgca acgagcggac ggcctggtgg actgcaacca gtgacgatga gcaggccgtc
     8041 tcgatcgcca tggacgggcc gcgcaaattg aggaagtagg agattcggcc ggccgccacg
     8101 ctcagcgcgg tcccggtgcc gaagtacttg tctatctcgt cgagcttgcc gctgttgccc
     8161 gcgagcatgg cgtactcact ggaactgatc ccaatgaaca ctcccgtcgc gctgcgctcg
     8221 aggttcctgt gcacttgccc ggcgctctcc agcgcttccc agctcacctc gagcagcagg
     8281 cgttgctgcg gatccatgct cttcgcctcg cgtggagtca cgcgaaagaa cttctcgtcg
     8341 aacagatcta tgtccctgag gaaagctccg cgcctcgagt acatcttgcc gggggttcct
     8401 ggagttggat cgtagaaggc atcagcatcc cagcgctcac gcggaatttc agaaatcgcg
     8461 tctgctttgg agatcagcgc attccagaag gattgtggat taaccgactg cgggaagcgg
     8521 cacgccatgc caacgacagc gatgtcgcgc tcgctggcgc gcgccgctgc gtgtatgaga
     8581 tctcggccgg actccggttg agttttcatc ttgacgacgc ctatgcgatg cgtagcggta
     8641 tggtctaacg tttgcacgga ctccgtgcct tgacaacaca cgctggaggc aatttagata
     8701 gcactgttcg tccgccccgt cgcttcacga gattccgacc gaatgctgca acgtgtggtt
     8761 tctgcaatgc gcgcgcagct gttcggcatc agcggcgatc aggcgttgtt tcgccgacgc
     8821 ggattcaaga gcgcgagagc gaagagtcag cacgtgctgg aggacatcgg gaagactttc
     8881 atcgatggct acaattttgc ggtcagatcg gccgatccga cgctgcaatc cgcccggcta
     8941 gacagcattc cggttgacat gcacggattc ttcgttgagg gcgcgtcgat gggcttcgcg
     9001 ctgtttgaca ttgtatctcc gtggcccacg cgattcttcc aggagttcat ccgtcaggca
     9061 gcgtcgccac acatttatat ggcctacgtc ggagcgggct gggcgctcgc gcgtacgtcg
     9121 ctgaggctgt cgtggcgact gggcaagacc gacccgcttc tgcggtggct gatgtttgat
     9181 ggcctcggct tccatcatgg ctacttt
//
LOCUS       DATMRM010000128         3299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_58553, whole genome shotgun sequence.
ACCESSION   DATMRM010000128 DATMRM010000000
VERSION     DATMRM010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58553"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..622
                     /locus_tag="VNZ06_03645"
     CDS             176..622
                     /locus_tag="VNZ06_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HWW19876.1"
                     /translation="MAKIFRPDVTVADLQKVSERTLVDSLGITLTELGDDFLRGTIRV
                     DHRNCQPMRILHGGASVALAETLASLAANSVSSIDREKAYCVGQEINANHLRRAPEGT
                     LVIGTARPFHIGSQSQVWGIELVNEAGQRTCVSRITLAVVKRSSRA"
     gene            complement(626..2902)
                     /locus_tag="VNZ06_03650"
     CDS             complement(626..2902)
                     /locus_tag="VNZ06_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWW19877.1"
                     /translation="MRSISMRVAARGPHDLRRPVVVWSVILLSVFSLLAARSIRAADI
                     GAVRGIVHDSEHRPIAHAGVVLQSANSEWRQATASSADGVFVFANVPFGDYRLEVHQS
                     GYTVSAQSVTVTSGVLPNLHIQLSATPSSELAAITVTATPEPGPVPSATPTTLVDRND
                     VQLTPGADRSNSLALITDFVPGAYFVHDQLHVRGGHQTTWAVDGVEIPNTNIASNLGP
                     QIDPKDIDYLEIQRGSYQAELGDRTYGVFNVVPRTGFEGHNRGEVIVSGGSDGQTNDY
                     VSFGSHTDKLAYYVSLNGNRSDLGIETPTAQILHDGEDGYGGFATLIDNLSADDQIRL
                     VVQARHDDYQIPNSAGDLLDDVQRESDAFAILSWVHSFSADATLTSSLFYHYNRADFD
                     GAPTDSPIATTDGRASSYLGGQETLRWHLWRNDLQIGVLGFGQQDNELFQILFNNDAN
                     APVFALAHPTGTLLAGFVQDTLRATEWLTLMAGVRETYFSGHVTENATSPRIGITIQL
                     PAMAWVVHGFWGKFYQAPPLDTLSGPLLQFASNNDTAFLPLHGERDTEYQYGLSVPCR
                     GWRIDLDRFRTEARNFFDHNPVGNSDIFLPLTITGALIEATELTVRSPRFWNAARFHL
                     AYSNQTADGFGAITGGLTEFSPPAGPFALDHDQRTTISSGFELKLPWKTFVSMNLSYG
                     SGFANGGQGPSHLPSHAELDLSLGREFLPNLTASLTALNLANKHLLIDNSLTFGGVHY
                     NSPLQVYAEIRYGFAGAH"
     gene            complement(3022..>3299)
                     /locus_tag="VNZ06_03655"
     CDS             complement(3022..>3299)
                     /locus_tag="VNZ06_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19878.1"
                     /translation="LLRASIPVGFMPGAGGQLMLCHDGMHVPNAPHAHYEHCPFGGGP
                     ASCPYELRSVVAHVTLIEQQASARGAPVLISIRLVHLPQPRGPPALV"
BASE COUNT          614 a          982 c         1107 g          596 t
ORIGIN      
        1 cgctgaggcg gtactgcacg gaaccgaata cacactgggc gatgtgaccg gcgcaagcct
       61 cggcggcggg tggatcacgc ggctcgtgga ctggggcttc acccgcggcg caggtcgcga
      121 gccgcccgtt gcggccgtcc agccgaccgc ctgaaacgcc gcgacgaggc acacgatggc
      181 aaagatcttc cggcccgacg tgaccgttgc ggacctgcag aaggtcagcg agcgcacgct
      241 ggttgacagt ctgggtatca cgcttaccga gctgggcgat gacttcctgc ggggcacgat
      301 acgggtggat catcgcaatt gtcagccaat gcgcatcttg cacggcgggg cttcggtcgc
      361 gttggctgag acactcgcca gcctggcggc caattcagtc agctcgattg atcgcgaaaa
      421 ggcgtactgc gtggggcagg agatcaacgc caatcatctg cgccgcgcgc ccgaaggcac
      481 actggtgatt ggcaccgctc gtccgtttca catcggatcg caaagtcagg tgtggggcat
      541 cgaactcgtc aacgaggcag ggcagcgtac ctgcgtctcg cgtatcacgc tggcggtggt
      601 caagcgctcg agccgcgctt gacgctcagt gcgctccggc gaagccgtag cggatctcgg
      661 cgtacacctg cagtggtgag ttgtagtgca caccgccgaa ggtcaggctg ttgtcgatca
      721 gcagatgttt gttcgcgagg ttcaaagcgg tcagcgaggc ggtcaggttg ggcagaaact
      781 cgcgcccgag gctgaggtcc agctcggcgt ggctcggcag atggctcggt ccctgaccgc
      841 cgttggcgaa cccggagccg taggataggt tcatcgacac gaacgtcttc cagggcagtt
      901 tcagctcgaa gccgctgctg atcgtggtgc gctgatcgtg atcgagtgcg aacgggcctg
      961 cgggcggact gaactcggtc aatccgccag tgatcgcacc gaagccgtcg gcggtctgat
     1021 tggaatacgc cagatgaaac cgcgcggcgt tccagaagcg tggcgagcgc accgtcagtt
     1081 cagtggcctc aatcaacgct ccggtaatcg tgagcggcag gaagatgtcc gagttgccga
     1141 ccggattgtg gtcgaagaag ttgcgcgcct cggtgcggaa gcgatcgagg tcgatcctcc
     1201 agccgcggca cgggaccgac aggccgtact ggtattccgt gtcacgctca ccgtgtagcg
     1261 gcaggaaggc cgtgtcgttg ttgctcgcga actgcagcag cggtccggac agcgtgtcga
     1321 gcggcggggc ctggtagaac ttgccccaga atccgtgtac gacccatgcc atggccggta
     1381 gttgtatcgt tatgccgatc ctgggggagg tggcgttctc ggtcacgtgg ccggagaagt
     1441 aggtttcccg aactcccgcc atcagggtca gccactcggt ggccctgagc gtgtcctgaa
     1501 cgaagccggc cagcagcgta ccggtcgggt gcgcgagcgc gaacaccggc gcgttggcgt
     1561 cattgttgaa gaggatctga aagagctcgt tgtcctgctg gccgaagccg agcacgccga
     1621 tttgaagatc gttcctccac agatgccatc gcagcgtttc ctgtccaccc agataactcg
     1681 aggcacgccc gtcggtggtg gcgatgggag agtccgtggg ggcgccgtca aaatcagccc
     1741 ggttgtagtg atagaacagt gaactcgtca gtgtcgcgtc ggcgctgaac gaatgtaccc
     1801 acgacaggat ggcgaaggcg tcgctctcgc gctgtacatc atccagcaga tctcccgcgg
     1861 aattgggaat ctgatagtca tcgtgccgtg cctgcaccac cagacggatc tgatcgtcgg
     1921 cgctgaggtt gtcgatcagg gtggcaaagc caccgtaacc gtcctcgccg tcgtgcagaa
     1981 tctgcgcagt cggcgtctcg atgccgaggt cgctgcgatt gccgttgaga ctcacatagt
     2041 aggccagctt gtcggtgtgg ctcccgaagc tgacgtagtc attggtctga ccatcgctgc
     2101 caccgctgac aatcacttca ccgcgattgt gtccttcgaa tccggttctg ggcacgacat
     2161 tgaacacgcc ataggtgcga tcgcccagct ccgcctggta gctgccacgc tggatttcca
     2221 gataatcgat gtccttcggg tcgatctgtg gacccaggtt cgaggcgata ttggtgttcg
     2281 gaatctccac gccgtccaca gcccaggtgg tctgatgtcc gccgcgcacg tgtagctgat
     2341 cgtgtacgaa ataggctccc ggcacgaaat ccgtgatgag ggcgaggctg ttgctgcgat
     2401 ccgcgccggg agtcaactgc acgtcgttgc gatccacgag cgtggtcggc gtggcggatg
     2461 ggactggccc aggctcaggc gtcgccgtga cggtgatggc cgcaagctcg gaactcggcg
     2521 ttgccgagag ctggatgtgc agattgggca agactcccga ggtgacggtg accgactgcg
     2581 cggacacggt gtagccgctc tgatgaacct cgagtcgata gtctccgaac ggcacgttcg
     2641 caaacacaaa aacgccatcg gcagagctgg cggtggcttg tcgccactcc gagttcgccg
     2701 attgcagcac cacacctgca tgcgcgatgg ggcgatgctc ggagtcatgc acgatcccgc
     2761 gcacggcacc gatgtcggcc gcgcggatgc tgcgagcggc cagcaaactg aaaactgaca
     2821 acaggatcac cgaccaaacg accacgggcc ggcgcaggtc gtgcggcccg cgcgcggcca
     2881 cgcgcatgga tatcgaacgc atctggttct ccgtcctcgc cgtgcaccgt cactgcgccc
     2941 ggcgattgaa tgcaatccgt cggaaaatga ggcttgcgcg ccgtgcgaat gcgcacgacc
     3001 tgcctcaacg cggcatggat gtcagacgag tgcgggaggg ccgcgcggtt gcggcagatg
     3061 gaccaggcgt atggaaatca gaaccggtgc gccgcgcgcg ctggcttgct gctcgatcaa
     3121 ggtcacgtgt gcgacgaccg agcgcagctc gtacgggcag gacgcggggc caccgccgaa
     3181 tgggcagtgc tcgtagtgcg cgtgcggggc attgggaacg tgcatgccgt cgtgacacag
     3241 catgagctga ccgcccgcac ccggcatgaa gcccaccgga atggatgcgc gcagcaaca
//
LOCUS       DATMRM010000129         3575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_995778, whole genome shotgun sequence.
ACCESSION   DATMRM010000129 DATMRM010000000
VERSION     DATMRM010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995778"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VNZ06_03660"
     CDS             <1..509
                     /locus_tag="VNZ06_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW19879.1"
                     /translation="ITILSALPLSIGGAVLGLAAFGSSFSLFAVLGMLMLMGIVAKNS
                     ILLVDFAIEEMRAGKDRMTALLEAGHKRARPIVMTSAAMIAGMLPAALGIGGADRFQS
                     DMADAVIGGLITSTGLTLVMVPAVFTWVDDLERWLGGFFARGFLNPENRTAVVVSHSE
                     PVTDSQHA"
     gene            502..1437
                     /locus_tag="VNZ06_03665"
     CDS             502..1437
                     /locus_tag="VNZ06_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWW19880.1"
                     /translation="MPDRAAGPLYGAIEAGGTKFCCALAGGVSDLRASERFATTTPDE
                     TFAKVIDFFSSCERTHGPISAFGIGCFGPLDLDVRSASYGRILTTPKPGWSHCDLRAR
                     LRERFAIPIGLDTDVNAAALAEWDHAASGGLRSLVYVTVGTGIGGGAVIAGRSLQGPW
                     HPEMGHIRVVRHPEDTAFAGVCPFHGDCLEGLASGPAITARWGAEMKALLQDTRACRI
                     IGSYLGQFAATIALMLAPDRIVFGGGVMNAGALLSQIRSSCRQQLAGYLSHPLLQQTL
                     GQFIVGPALAERSGICGAILLATASHRASHAGAAE"
     gene            1418..1876
                     /gene="tadA"
                     /locus_tag="VNZ06_03670"
     CDS             1418..1876
                     /gene="tadA"
                     /locus_tag="VNZ06_03670"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HWW19881.1"
                     /translation="MPAPPNDIDFMRAALERARAALSAGEVPVGAVLVRHDAIIASGT
                     NRPIAACDPTAHAEIEALRAGARAQGSYRLADTTLYVTLEPCPMCAAALVHARVRRVV
                     FGAFDPRVGAAGSAIDIFKLEGLNHRVDVFGGVLQQQCSALLQGFFAERR"
     gene            complement(1893..3278)
                     /gene="mltF"
                     /locus_tag="VNZ06_03675"
     CDS             complement(1893..3278)
                     /gene="mltF"
                     /locus_tag="VNZ06_03675"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HWW19882.1"
                     /translation="MTAAGAIALLLASCTASQPALQEIRARGELRVVMLNAPTSYYLG
                     AHGPQGFEYRLVSAFAQQLGVRLSIQAVADNAAVRAALRHGRADMAAAQISADQRWLS
                     IGEATESYENLAQLVVQGRGKTRPRDVTGLRGAHVVVLAGSAQLALLRSIQSNGVPGL
                     NWTEVPPDQVDLLKLVSDGDADYAVMDANEFEFAQHLYPDSSIAFKLPDTRPLQWIVR
                     SGGADLVQAANHFFSEAKASGELARIAQNASAEAVGFDYTDAHRFQADMSERLPELRT
                     QFEQASQQTGLDWRLIAAVGYQESRWQMQAASGDGARGIMMLTNDAASTVGVTDRADQ
                     WQNILGGAKYLAQAFASIPKRIPEPDHTWFALAAYNAGYGHLEDARVLAQMRGKNPDS
                     WDDVREQLPLLAEEAWYTKLKRGYARGWEPARFVDQVRQYLAVLEWSDAATNSVAQLH
                     TAYAEPLQTLN"
     gene            3353..3445
                     /locus_tag="VNZ06_03680"
     tRNA            3353..3445
                     /locus_tag="VNZ06_03680"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3388..3390,aa:Ser,seq:cga)
BASE COUNT          573 a         1152 c         1179 g          671 t
ORIGIN      
        1 cgatcacgat cctgtcggcg ctgccgctgt cgataggtgg cgcggtgctc ggactggctg
       61 cgttcggcag ttcgttctca ttattcgcag tactcggtat gctgatgctg atgggtatcg
      121 tggcgaaaaa ttcgatcctg ctggtcgatt ttgcgatcga agaaatgcgc gccggcaagg
      181 accggatgac cgcgctgctg gaggccgggc acaagcgcgc gcgaccgatt gtcatgacct
      241 ccgcggcgat gatcgccggt atgctgcctg cggcactggg aattggcggc gcagaccgct
      301 ttcagagtga tatggctgat gcggtgatcg ggggactcat cacatcgacc ggcctcacgc
      361 tcgtcatggt gccggccgtc ttcacgtggg tcgatgatct ggagcgctgg ctcggaggct
      421 tcttcgcccg tggattcctc aacccggaga accggaccgc ggttgtcgtg tcgcactctg
      481 agccggtcac cgattcacaa catgcctgat cgggcggctg gcccgctgta cggggcgatc
      541 gaggcgggcg gtacgaagtt ctgctgtgcg ctggcaggcg gcgtcagtga cctgcgcgcg
      601 tccgagcgct ttgccaccac tacgccggat gagaccttcg ccaaggtcat cgacttcttc
      661 agctcttgcg agcgaaccca cggaccgatc agcgccttcg gcatcggctg cttcggcccg
      721 ctggacctgg atgtgcgctc ggccagctat ggccggatcc tgaccacgcc caaaccgggc
      781 tggagccact gcgatctgcg cgctcgactg cgcgagcgct tcgccattcc gatcgggctg
      841 gacacagacg tcaacgcggc ggcgctggcg gagtgggacc acgctgcctc gggtgggctg
      901 cgctcactgg tctatgtgac tgtcgggacc ggcatcggtg gcggggcggt gatcgccggg
      961 cgcagcctgc aggggccgtg gcatccggag atgggtcata tccgggtcgt acgacacccc
     1021 gaggacacgg ctttcgccgg ggtctgtccg tttcatggtg attgtctgga aggtctggcc
     1081 agcggacctg cgatcacggc gcgctggggc gctgaaatga aggcgctgct tcaggatacg
     1141 cgcgcctgcc gcatcatcgg cagctatctc ggacaattcg cagcgacgat cgcgctgatg
     1201 ctggctcccg atcgcatcgt gttcggcggc ggcgtcatga acgccggcgc gctgttgagc
     1261 cagatccgca gcagctgccg gcagcagctg gcaggctacc tctcacaccc gctgctgcag
     1321 cagacactcg ggcagttcat cgtcggcccg gcgcttgctg aacgctcggg tatctgcggc
     1381 gcgattcttc tggcgaccgc cagtcacagg gcatcgcatg ccggcgccgc cgaatgacat
     1441 tgatttcatg cgggcagcac tcgagcgcgc acgcgcggcg ctcagcgccg gcgaggtgcc
     1501 ggtcggcgcg gtgctggtgc gccacgatgc catcatcgcg agcggcacca atcgtcccat
     1561 tgccgcctgc gatccgaccg cgcacgccga gatcgaggcg ctgcgcgcgg gcgcacgggc
     1621 gcagggcagc tatcgtttgg cggacacgac cctgtatgta acgctcgaac cgtgtccgat
     1681 gtgcgccgca gccctggtgc acgcccgggt gcgccgcgtg gtgttcggcg cattcgatcc
     1741 gcgcgtcggt gccgccggca gcgcgatcga catcttcaag ctcgaaggcc tgaaccatcg
     1801 cgttgatgtg ttcggtgggg tactgcagca gcagtgcagc gcgctgctgc aaggcttctt
     1861 cgccgagcga cgctagcccg tcaaggggcc ggctaattga gcgtctgcag cggctcggcg
     1921 tacgcggtgt gcagctgcgc caccgagttc gtggcggcat cgctccattc gagcactgcg
     1981 aggtactggc gcacctgatc gacaaagcga gctggctccc agccgcgggc atagccgcgc
     2041 ttgagtttgg tgtaccacgc ctcttccgcc agaagcggca gctgctcgcg cacgtcgtcc
     2101 caggaatccg ggttcttgcc gcgcatctgc gccagcacgc gcgcatcttc cagatgtccg
     2161 tagccggcgt tgtacgcggc cagcgcgaac caggtgtgat cgggctccgg aatgcgtttc
     2221 gggatgctgg caaaggcctg cgccaggtat ttcgcgccac cgagaatatt ctgccactgg
     2281 tcggcgcggt cagtgacgcc gacggtgctg gccgcatcat tggtcagcat catgatgccg
     2341 cgtgcaccat cgcctgacgc cgcctgcatc tgccagcgcg attcctgata gccgaccgcc
     2401 gcgatcaggc gccagtcgag cccggtctgc tgtgaggctt gctcgaactg cgtgcgcagt
     2461 tcgggcagac gctcgctcat atcggcctga aagcgatgcg catcggtgta atcgaagcca
     2521 accgcctcgg cgctggcgtt ctgcgcgatg cgcgccagtt cgcccgaggc cttggcctcg
     2581 gagaaaaaat ggttggccgc ctgtaccaga tcggcgccac ccgagcgcac gatccattgc
     2641 agcggccgcg tgtccggcag tttgaaagcg atgctcgaat ccgggtacag gtgctgtgcg
     2701 aattcaaatt cattcgcatc catgaccgca tagtcggcgt cgccgtccga taccagtttg
     2761 agcagatcca cctgatcggg cggcacttcc gtccagttca gccccggcac gccgttgctc
     2821 tgtatcgagc gcagcagagc cagttgcgca ctgccggcca gcaccaccac atgggcgccg
     2881 cgcaggccag tcacgtcgcg cgggcgggtc ttgccccggc cctgcacgac cagctgcgct
     2941 agattctcat agctttcggt ggcctcaccg atgctgagcc agcgctgatc ggcgctgatc
     3001 tgcgcggccg ccatatcggc gcgcccgtgc cgcagggcgg cccgtacggc ggcgttgtcc
     3061 gcgaccgcct ggattgacaa tctgacaccc agctgctgcg caaatgcgct gaccaggcgg
     3121 tattcaaaac cctgcggtcc gtgcgcgccg aggtagtagc tggtcggcgc attgagcatc
     3181 accacgcgca attcgccgcg cgcgcggatc tcctgcagtg ccggctgcga agccgtgcag
     3241 ctcgccagca acagggcgat cgcgcctgcc gcagtcactc ggcgcgcgaa tcgtcggcta
     3301 agttggggtc tcattgcacg catctgtggg ctagaatagc tcccctcccc acggagaggt
     3361 accgaagcgg tcataacggc gccgactcga aatcggatgg tcgggttaaa ccggcacgtg
     3421 ggttcgaatc ccaccctctc cgccaatttt ccgcgacaat tctcagtacc cgttgaacgt
     3481 tttgagtgcc agtgcgcagg tatctcggaa attgctggcg ctgcgacaaa ggcaaattgt
     3541 cgttacactt gttggtggag aggacataac tggcg
//
LOCUS       DATMRM010000130         3022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2848751, whole genome shotgun sequence.
ACCESSION   DATMRM010000130 DATMRM010000000
VERSION     DATMRM010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2848751"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..800
                     /gene="can"
                     /locus_tag="VNZ06_03685"
     CDS             156..800
                     /gene="can"
                     /locus_tag="VNZ06_03685"
                     /inference="COORDINATES: protein motif:HMM:NF007756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HWW19883.1"
                     /translation="MNRTADLETLLARNRQWASDLTQSDPQFFSRLALQHAPRYLWIG
                     CSDSRVPANQILGLVPGEIFVHRNVANVVVHSDLNCLSVVQYAVEVLRVRDIIVCGHY
                     GCGGVAAALSDQKLGLIDNWLRHVQDVGRRHAAVLDAAGPAQMQLNRLCELNVLTQTV
                     NVAETTVVQDAWARGQELAVHGLIYGLDDGILRQLGLSLTSDAALRAYKASALP"
     gene            822..2645
                     /gene="uvrC"
                     /locus_tag="VNZ06_03690"
     CDS             822..2645
                     /gene="uvrC"
                     /locus_tag="VNZ06_03690"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HWW19884.1"
                     /translation="MTETTFDPKAYVATLPHRPGVYRMYAADGELIYVGKAARLRDRV
                     SSYFVKGLPSARIAAMVKRIARIEVTVASTEPEALLLESNLIKAHRPRYNIVLRDDKS
                     FPYILCQPGHDFPRLVFYRGPKQQGSRQFGPFPSAYIVRDVLQHLQKVFKIRNCRDSF
                     FANRSRPCLQHQIGRCSAPCVGLIDQQSYARDLQGAIGVLEGRNAEVVAEWQRRMETA
                     AASLQFEEAARLRDQLRELQEIRAQQLANAARRTDTDVIAMVGEPGQYAICVLPVREG
                     QNLGTASHFPGSALSEPAETMADFLLQYYSHEGAPPEILINVELADHDAVAEALERAA
                     GRSLQLRVPQRGLGERWMEQALENANNALRMRRLKADVAQEGLQLLAQVLDLPEAPAR
                     VECFDVSHTAGEGTVASCVVFTPEGARKADYRRYNIEGVAAGDDYAAMHQALKRHGAH
                     IVKGDRPAPDLLLIDGGPGQLDAAVAALAEVGAQGLAVVGVAKGADRRPGQERLFLPG
                     QPIPVTLAADSAALHFIQRVRDDAHRFAITGHRRRRARRYKESVLETVPGLGPARRRA
                     LLTHFGGLQGVMRAGIADLEKVAGVGAAMARSLYDHLHPGA"
     gene            2645..>3022
                     /gene="pgsA"
                     /locus_tag="VNZ06_03695"
     CDS             2645..>3022
                     /gene="pgsA"
                     /locus_tag="VNZ06_03695"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HWW19885.1"
                     /translation="MIWNLPNVLTWLRILAIPLVVLLFLIGARHPLSLAYPISGLTFA
                     AAAITDSLDGYLARRLGQTTPLGQFLDPVADKLIVATALVLIVSRDTSEVIVLTAAVI
                     IGREIAISALREWMAGIGQRTAVA"
BASE COUNT          481 a          927 c         1061 g          553 t
ORIGIN      
        1 accggcgagg acgtgaccga gtgcaccggc ggtgcacgcg gcctgaccga cgccgatctg
       61 cagcgggcgt atcgttcgca ggtcgatcca cgcctgaact acgagcaggc gctggagctg
      121 gcgatgctga tcgccgagcg cagcaagcgt cagccgtgaa ccggaccgcg gatctggaaa
      181 cactgctggc gcgcaaccgg cagtgggcca gcgatttgac gcagtccgat ccgcagttct
      241 tttcgcgtct ggcgcttcag cacgcaccgc gctatctgtg gatcggatgc tccgacagcc
      301 gcgtaccggc gaatcagatc ctcgggctgg tgccggggga gatcttcgtt caccgcaacg
      361 tcgccaatgt ggtggttcac agcgatctga actgtctgtc ggtcgtgcag tacgcggtcg
      421 aagtactgcg ggtgcgcgac atcatcgtgt gtgggcacta cggctgcggc ggcgtggccg
      481 cagcgctgtc ggatcagaag ctcgggctca tcgacaactg gctgcgccat gtccaggacg
      541 tggggcggcg gcatgcggct gtcctcgacg cggccggtcc ggcgcagatg cagttgaacc
      601 gcctgtgcga actgaatgtt ctgacacaga cggtcaatgt ggcggaaacc accgtggtgc
      661 aggatgcctg ggcgcgcggc caggaactgg cggtgcacgg tttgatctat ggactggatg
      721 acgggattct gcgccagctg gggttgagcc tgacttccga tgcggcgctg cgggcttaca
      781 aggcgagcgc gttgccctag cgggcttggc gcgggccgcc aatgaccgaa accactttcg
      841 atcccaaagc ctacgttgcg accctgccgc accggcccgg ggtctatcgc atgtatgcag
      901 cggatggcga gctgatctat gtcggcaagg ccgcgcggct gcgcgatcgc gtcagcagct
      961 acttcgtcaa ggggctgccc tcggcgcgca tcgctgcgat ggtcaagcgc atcgcccgca
     1021 tcgaggtgac ggtcgccagc accgagcccg aggcgctgct gctcgagagc aatctcatca
     1081 aggcgcatcg tccgcgctac aacatcgttc tgcgcgatga caaatccttt ccgtacatcc
     1141 tgtgtcagcc cgggcacgat ttcccgcggc tggtgttcta tcgcggtccc aagcagcagg
     1201 gcagccgcca gttcggtccg ttccccagcg cttacatcgt gcgcgacgtg ctgcagcacc
     1261 tgcagaaggt gttcaagatc cgcaactgcc gtgattcgtt tttcgccaat cgcagccgtc
     1321 cgtgcctgca gcatcagatc ggtcgctgtt cggcgccgtg tgtgggtctg atcgaccagc
     1381 agtcctacgc gcgcgacctg cagggcgcga tcggtgtgct cgaaggacgc aacgccgaag
     1441 tggtggccga atggcagcgc cgcatggaaa cggcggccgc gagtctgcag ttcgaagagg
     1501 ccgcgcgtct gcgcgatcag ctgcgcgagc tgcaggagat tcgcgctcag cagctggcga
     1561 atgcggcgcg gcgcaccgat acggatgtga tcgccatggt cggtgagccc ggccagtatg
     1621 cgatctgcgt gctgccggtg cgcgagggtc agaatctggg cactgccagt cactttcccg
     1681 gcagcgcgct cagcgaaccg gctgagacga tggccgactt cctgctgcag tactacagcc
     1741 atgagggcgc gccaccggag atcctgatca acgtggagct ggccgatcac gacgcggtgg
     1801 ctgaggcgct cgagcgggcg gcgggtcgat cgctgcagct gcgggtaccg cagcgtggtc
     1861 tgggcgagcg ctggatggag caggcgctgg agaatgccaa caacgcgctg cgcatgcgtc
     1921 gtctcaaggc cgatgtggcg caggaggggt tgcagcttct ggcgcaggtg ctcgatctgc
     1981 ctgaggcgcc ggcacgggtg gagtgcttcg atgtgagtca cacggccgga gagggcacgg
     2041 tggcctcctg cgtcgtgttc acgcccgaag gggcgcgcaa ggccgactat cgccgctaca
     2101 acattgaggg agtcgctgcg ggtgatgact atgcagccat gcaccaggcg ctcaaacgcc
     2161 acggcgcgca tatcgtgaag ggcgatcgcc cggcgcccga tctgctgttg atcgacggcg
     2221 ggccaggtca gctcgatgct gcggtggcgg cactggccga ggtcggtgcc cagggcctcg
     2281 cggtggtcgg cgtcgccaag ggcgcggacc ggcgtcccgg gcaggagcgg ctgtttctgc
     2341 cggggcagcc gattccggtt accctggcgg ccgacagcgc ggcactgcac ttcattcagc
     2401 gggtgcgcga cgatgcgcac cgctttgcga ttaccggtca ccggcgacgg cgtgcgcgca
     2461 gatataaaga gtccgttctg gagaccgtcc ccggtctggg gccggcacgc cgtcgtgcgt
     2521 tgctgacgca cttcggcggc ttgcaggggg tcatgcgggc gggcatcgcc gatctggaaa
     2581 aagtcgccgg cgttggggcg gcgatggcgc gttcgcttta tgatcatctt catccggggg
     2641 cgtgatgatc tggaacctgc cgaacgtact gacctggctg cggattcttg cgattccgct
     2701 ggttgtgttg ctgttcctga tcggcgcgcg tcatccgctg agcctcgcct atccgatctc
     2761 cggactgacg tttgcggccg cggcgattac cgattcgctg gatggctatc tggcgcgacg
     2821 cctgggtcag accactccgc tcggacagtt cctcgatccg gtcgccgaca agctgatcgt
     2881 ggccaccgcg ctggtgctga tcgtcagtcg cgatacgagt gaagtgatcg tgctgaccgc
     2941 cgcggtcatc attggtcgtg agattgcaat ctcggcgctg cgtgaatgga tggccggcat
     3001 cggccagcgc accgcggtcg cg
//
LOCUS       DATMRM010000131         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4426153, whole genome shotgun sequence.
ACCESSION   DATMRM010000131 DATMRM010000000
VERSION     DATMRM010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4426153"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VNZ06_03700"
     CDS             <1..1060
                     /locus_tag="VNZ06_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19886.1"
                     /translation="NAGDTQGMLKGSRLDEVAVLTLTGVPFLPGKLQSAQGVDQLQLT
                     AQDPPTLPALRAGDAVTARVSLKDGRVFELVTVVQPARPKVTLIAKSVQLSSVTRESN
                     IHLGNQDELPQDARLTFSIRAQSPAAFAYDEKIEVANADQTVSALLSSSNGALTLENA
                     SVAVATLDAGKAFGPSAFGPLQFRVIGDGAAGDWQPLVTLVRLPALQELKCPATPELA
                     CKLSGTNLFLVDAVSTDAQFGHPVQVPDGFPGYSLPVPRPTDGQLYVKLRDDPSVVNR
                     AILVAQVLPPTPEEAARAAARHAAAAQTEASTTLLPAPPASTPAPVPPSSPETLPRPP
                     SDSGSVNGEQPRPSGAGL"
     gene            complement(1066..>2853)
                     /locus_tag="VNZ06_03705"
     CDS             complement(1066..>2853)
                     /locus_tag="VNZ06_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWW19887.1"
                     /translation="QAVLDPQVSPRGRYVSFVRDQNLWVIDLTSGQERQLTTDGGGTI
                     HNAEAEFVAQEEMSQTSGYWWAPDDSTIAYKRFDESAVPIARRFEIYAERVDVIEQRY
                     PAAGDPNVTVKLGLLAPTGGTTRWVDLGPNPDLYLVRADWTPDGKQFAYQLLSRDQKR
                     LDLVLVTAATLQRRTLVTESSQTWVNLSKDLHFLRHKPEFLWSSERSGSKHLYLIGLD
                     GKLRHALTQGNWQIDELLAVDERKGLVFLASNKDAIIDSQIYTVRLDGSDAAHPHRLS
                     TGDGWHDAQFARDADRASLYVDTFSNSNTPPQVSINAPDGHRLAWIEENPLDSNHPYW
                     PYHEQHVLPEYGHIQADDGQELEYMLMKPPGFDPSRRYPVFLNVYGGPTVQNVKRTWP
                     ALFDEYMAQRGFIVFTLDNRGSSRRSRAFQDPIYRRLGEIEVRDQLSGARWLQTQPWV
                     DAKHIGVFGWSYGGYLTLMLLGKGSDVLAAGFAVAPVTDWSLYDTCYTERYLSTPKDN
                     AQGYSQSSVFSALDGLKSPLFLAHGMADDNVLFVNSTRLMSELQRREVQFQLMTYPGA
                     KHGLSTPQMKIHIYTAIHRYMEAKLKPAH"
BASE COUNT          512 a          899 c          912 g          530 t
ORIGIN      
        1 caacgcgggc gacacgcagg gcatgctcaa gggtagccgc ctggacgagg tggccgtcct
       61 caccctgacg ggtgtgccct tcctgcccgg caagttgcag tcggcccagg gcgtcgatca
      121 attgcagctg acggcgcagg atccgccgac cctgcctgcg ctgagggcgg gtgatgccgt
      181 aacggccaga gtgtctctga aagatggccg ggtgttcgaa ctggtcaccg tggtgcagcc
      241 cgcgcgccca aaggtgacgc tgattgccaa gagcgtgcag ctctcttcgg tcacccgcga
      301 gagcaacatt catctcggca atcaggatga actgccgcag gatgcgaggc tgacgttctc
      361 gatacgggcg cagtcgcctg ccgcgttcgc ctacgatgaa aagatcgagg ttgcgaatgc
      421 cgatcagacc gtctctgcac tgctgagctc gagcaacggc gctctgacgc tcgagaacgc
      481 ctcggtggcg gtcgcgacgc tcgatgctgg caaagcgttt ggtccttcgg ccttcggacc
      541 gctgcagttt cgcgtcatcg gcgatggcgc tgcgggggat tggcagccac tggtgacact
      601 ggtgcgcttg ccggcgttgc aggaactgaa atgcccggcg actccggagc tggcctgcaa
      661 actctccggt accaaccttt tcctggtgga cgcggtgtcc actgacgcac agttcggaca
      721 tccggttcag gtaccggacg ggttccccgg ctacagctta ccggtgccgc gtccgaccga
      781 cgggcagctg tatgtgaaac tgcgcgacga cccgtccgtg gtcaaccgcg ccattctggt
      841 ggcgcaagtg ctaccgccga cgccagagga agccgcccgg gcggcggcgc gccacgccgc
      901 ggcggcacag accgaggcat cgacaacatt gctgcccgcc ccgccggcat cgacacctgc
      961 gccggtgccg ccgagctcgc ccgagacctt gccccgcccg ccgtcggact ccggaagcgt
     1021 gaatggtgag cagccccggc cgagcggtgc gggtctctag tggcttcaat gcgccggctt
     1081 cagcttggct tccatgtagc ggtggatcgc cgtgtagata tgaatcttca tctgcggcgt
     1141 cgacaacccg tgtttggccc ccggataggt catcaactga aactgcactt cgcgacgctg
     1201 cagctctgac atcagtcggg tcgaattgac gaacagcaca ttgtcatcgg ccatgccatg
     1261 ggcgaggaac agcggcgact tcagcccgtc caacgctgag aatacggagg attgcgaata
     1321 gccctgcgcg ttgtccttcg gtgtcgacag atagcgctcg gtgtagcagg tgtcatacag
     1381 cgaccagtcc gtcaccggcg ctaccgcgaa accggcggcc agcacgtccg atcccttgcc
     1441 gagcagcatc agcgtcaggt agccgccata gctccagccg aagacgccga tgtgcttggc
     1501 atcgacccac ggctgcgtct gcagccagcg ggctccgctc agctgatcgc gcacctcgat
     1561 ttcaccgaga cgacggtaga ttggatcctg aaacgcacgg ctgcggcgcg atgagccgcg
     1621 attgtcgagc gtgaacacga tgaagccgcg ctgcgccatg tactcatcga acaaagccgg
     1681 ccaggtgcgt ttcacgttct gcaccgtggg tccgccataa acgttcagga acaccgggta
     1741 gcgcctggaa ggatcgaagc ccggaggctt catgagcatg tactcgagtt cctgcccgtc
     1801 gtcggcctga atgtggccgt actcgggcag cacatgctgc tcgtgatagg gccagtacgg
     1861 atgattggaa tcgagcggat tctcttcgat ccacgccagg cgatgaccgt cgggtgcatt
     1921 gatgctcacc tgcggcggcg tgttcgaatt ggagaaggta tcgacgtaga gggaagcgcg
     1981 atcggcatcg cgcgcgaact gagcgtcatg ccagccatcg cctgtgctga ggcgatgcgg
     2041 atgggcggca tcgctgccat ccagacgcac cgtgtagatc tggctatcga tgatggcgtc
     2101 cttgttcgaa gcgaggaaga ccagcccctt gcgctcatcg accgccagca gttcatcgat
     2161 ctgccagttt ccctgcgtga gggcgtggcg cagcttgcca tccaacccga tcaggtacag
     2221 gtgcttgctg ccgctgcgct ccgatgacca gaggaactcc ggcttatgtc tcaggaaatg
     2281 caggtccttg ctcagattga cccaggtttg ggagctctcg gtgaccagcg tcctgcgctg
     2341 cagcgttgcg gccgtcacca gcaccagatc caggcgtttc tgatcgcgcg acagcagctg
     2401 ataggcgaac tgcttgccat ccggtgtcca gtcggcgcgt accaggtaaa ggtcaggatt
     2461 gggtccgagg tccacccagc gcgtcgttcc accggtcggc gcgaggagcc cgagtttaac
     2521 ggtgacattc ggatcgccgg cggcggggta gcgctgctcg ataacatcga cgcgctcggc
     2581 gtagatctcg aagcggcgcg cgatcggtac cgcgctctcg tcaaatcgct tataggcgat
     2641 cgtggagtcg tccggtgccc accagtagcc gctggtctgc gacatttctt cctgcgcgac
     2701 aaactcggcc tcggcgttgt gaatcgtgcc gccgccatcg gtggtgagtt gccgctcctg
     2761 gccggacgtc aggtcgatca cccacagatt ctgatcacgc acgaatgaga cataccggcc
     2821 cctgggcgac acctgcgggt ccagcaccgc ctg
//
LOCUS       DATMRM010000132         4818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_689922, whole genome shotgun sequence.
ACCESSION   DATMRM010000132 DATMRM010000000
VERSION     DATMRM010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689922"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4704
                     /locus_tag="VNZ06_03710"
     CDS             <1..4704
                     /locus_tag="VNZ06_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HWW19888.1"
                     /translation="TWQQHCSEDPDPRSIGLLPTHLAYVIYTSGSTGQPKGVMIEHEN
                     IPRLVLSANYVDLCPNDAVLQISSLSFDISTFEIFGALLNGARLVIHPDGPVDLERLR
                     ETIQSNRVTVAMLATPLFHQAASEDPHIFDGMKQLLAGGDVIAAPDVNRVFAAIDGIT
                     FANAYGPSEATTLGAANRISKEGAPHEAVPIGRPVSNGSVYLLDRELQPVPRGAIGEI
                     FLGGHGIGRGYLGRPELTKERFLDSPFVKGDRLYRTGDLGRHLSDGKVEYLGRDDFQV
                     KILGFRVEPGEVESALLQHPLIREAVVVAREHGMDDKQLVAYYTTTGPEEPGVEALRA
                     YLLPKFPKHMVPAAYVQLSAMPLTPSAKLDRKALPAPDERSYGRATSALAPTGEMELQ
                     LAQLWSEVLLREQIGRHDNFFDLGGHSLLAVKLLARIHQDLHLRVTLQDLFTQPTLSE
                     FGLILSRLAAEGAHPLLIQDRSAPLKLSFSQLRLWFMAQMEGGDTAYHISLGVRLGGE
                     LIESALQHALDRLLVRHEVLRTSFEVVDGEVFQKIASEQKFLLVRHDVSALEQPQERV
                     DELVGVYSSKAFDLRRGPLIRGCLIRRAPQDHVLLLSMHHIVVDGWSVGILSRELSTL
                     YQAYAQGLEDPLRPLAVQYADYAAWQRRWLMGAVLTEQVEFWRETLSGAPALLELRTD
                     HKRPAQQSFEGALVDLEIDEALTVALRALSRRQGTTLFMTVLTAWALVLSRLSGQQDL
                     VIGVPSANRARKEIEGLIGFFVNTLALRIDLSGSPTLEQLLQRVKLVALAAQDHRELP
                     FEQVVDLIKPARSLAHTPIFQTMFAWQSEQQAKFDWPGMSAEPVRIARKKAKFDLTLS
                     LAESGGRIVGGLEYATALFDRQSIERFGGYLRRVLSEMVSEIGRVASSIPMLSDQERH
                     QLLVQWNETAVEYPRDQCVHQLFEAQVRRDAKAIAIVYENQTLSYAELNAQANQLAHR
                     LRELGVIPDSRVGICIQRSPEMVIGLLAILKAGAAYVPLDPGYPSERLAFMLSDSAPL
                     AVLTHEPAGAALQAAMAALSPQPALLNLSAPTWQQHCSEDPDPRSIGLLPTHLAYVIY
                     TSGSTGQPKGVMIEHENLVNYLGWALTAYRVGQGAGAVVNTSVSFDSTITSLYLPLLC
                     GRTVVLVREVDELEELELQLQRRPNLSLVKISPTHLNTLGQRFRVARPNLSLAAIVVG
                     GETLLPSTVELWQSIAPTIRLINEYGPTETVVGCSYFDIPVDWDAAAAVPIGRPIANT
                     KIYVLDLHQQPVPIGVVGEIYIGGSGLARGYWNRPELSRERFVQDPFNSDPAARLYRT
                     GDLGRWSASANLEFLGRNDRQVKIRGYRIELEEIEAQLLCHPKIAQAAVLTREDSLGE
                     KQLLAYYICNGAGHADASDLREHMRSALPKYMLPVAYVPLESFPMTPNGKLDRAALAP
                     PGTMAYGVREYEAPEGEVEQVLAEIWLDLLAIGRVGRLDHFFDLGGHSLLALQMISRV
                     EHRFQIEVPIRLVFMSPELAQLAARIDGLRHARLRDQLELGGDTIEDLLESVSSMSDA
                     AAREMLRRINLGTPQ"
BASE COUNT          964 a         1376 c         1494 g          984 t
ORIGIN      
        1 acctggcagc agcactgcag tgaggacccg gacccccgca gcatcggcct gctgccgact
       61 catctcgcct atgtcatcta tacctccggc tccacaggtc agcccaaggg cgtgatgatc
      121 gagcatgaga acattccgcg cctcgtgctg tcagcgaact acgtcgacct atgcccgaac
      181 gatgcggttt tgcagatctc atcgctctca ttcgatatct caacctttga gattttcggc
      241 gcgctgctca acggagcccg actggtgatt catccggacg gtccggtcga ccttgagcgg
      301 ctgcgcgaga cgattcagtc gaatcgggtg accgtcgcga tgctcgcaac gccactgttc
      361 catcaggctg cgagtgaaga cccacacatc ttcgacggca tgaagcaact gctggcaggc
      421 ggagatgtga tcgcggcgcc cgacgtcaat agggtgttcg ctgcgattga cggcattaca
      481 ttcgccaatg cttacgggcc ttccgaagcg acaacgcttg gggccgccaa tagaatctcg
      541 aaggagggcg ctccccatga ggcagtaccc atcgggcgac cggtctccaa cggcagtgtc
      601 tatctgctcg atcgggaact gcagccggtg ccccgagggg cgattggcga gatcttcctc
      661 ggcggacatg gcatcgggcg cggctatctc ggccgaccgg agctgacgaa ggagcgcttt
      721 ctcgatagcc cgttcgtcaa aggggacaga ctctaccgga ccggcgatct tggccgccat
      781 ctgtctgacg gaaaagtgga gtacctgggt cgtgacgact tccaggtcaa gattctgggg
      841 tttcgagttg agcccggcga ggtggagtcc gcgctcctac agcatcctct gattcgcgag
      901 gcagtggttg ttgcgcgcga gcatggcatg gacgacaaac agttagtcgc ctactacacg
      961 acgacggggc ccgaagagcc gggggttgaa gcgctgcgcg catacctcct ccccaagttt
     1021 ccgaagcata tggtgccggc tgcgtatgtg cagttgagcg ccatgccact gactccgagc
     1081 gccaaactcg accgcaaggc gctacctgca ccggacgagc ggtcatacgg ccgagcaact
     1141 tctgctctag cacccacggg tgagatggag ttgcaacttg ctcaactgtg gtcggaagtg
     1201 ctcctgcggg agcagatcgg gcgacatgac aatttctttg acctgggagg acattccctc
     1261 ctggcggtca agctgctggc gagaattcac caggacctgc acctgcgggt gacgttacag
     1321 gacctcttca cacaacccac gctgtctgag tttggcctca ttctgagtcg ccttgcagcc
     1381 gaaggggcac acccgttgct gatacaggat cggtcggcgc ctctgaagct ctctttttcg
     1441 cagttacggc tatggtttat ggcgcagatg gaaggcggag acacggcgta tcacatatcg
     1501 ctgggcgtaa ggctcggtgg ggagctgata gagtcggcgc tgcagcatgc actggatcgg
     1561 ctgttggtac gtcatgaggt tttgcgcacc agcttcgaag tggtggatgg cgaggtattt
     1621 cagaagatcg ccagcgagca gaagttcttg ttggtccggc acgatgtcag tgcgctcgag
     1681 caaccgcagg agcgagtcga tgagttggtg ggggtgtaca gttccaaggc ctttgacttg
     1741 cggcgtggac cgctgatccg tggctgcctg atcagacgcg cgccgcagga tcatgtattg
     1801 ctcctgtcga tgcaccatat cgtggtggat ggctggtcgg tggggatact ctcgcgcgag
     1861 ctcagcacac tctatcaagc ttacgcgcag ggtctggagg atcctctgag gccgctggcg
     1921 gtccagtatg cggactacgc agcctggcag cgacgttggc taatgggagc tgtgctgacc
     1981 gagcaggtcg agttctggcg cgagaccctg agcggtgcgc cagctctgtt ggagttgcgc
     2041 accgatcata agcgtccggc gcagcaaagt tttgagggag ccttggttga tctggaaatt
     2101 gacgaagctc tgaccgttgc actcagagcg ttgagccgac gacagggtac gacgttgttc
     2161 atgacggtat tgacggcctg ggctttggtt ctatctcgct tgtcgggcca acaggatctg
     2221 gtgatcggag tacccagtgc caatcgcgcg cgcaaggaga tcgaagggct gattggcttc
     2281 ttcgtcaata cgctggcgct gcgcatcgac ttgtcgggca gtcccacgct cgagcagcta
     2341 ctgcagcgtg tcaagctcgt tgcgcttgcc gcgcaggatc atcgagagct gccgtttgaa
     2401 caggtggtcg atctgatcaa gccggcgcgc agtctggcgc acacacccat ctttcagacg
     2461 atgtttgcct ggcagagcga gcagcaggcg aagtttgatt ggcctggtat gtcagcagag
     2521 cctgtgcgta tcgcccgaaa gaaagcgaaa tttgatctca ccttgagtct ggctgagagc
     2581 ggcgggcgga ttgtgggcgg cctggagtat gcgaccgcgc tcttcgatcg gcaaagcatc
     2641 gagcgatttg gcggctactt gcgccgtgtc ttgagcgaga tggtgtctga gatcggcagg
     2701 gtcgcatcga gcatcccgat gctatcggac caggagcggc accagttgct agtacagtgg
     2761 aatgaaacgg ccgtggagta tccacgcgat cagtgcgtgc accaactgtt cgaggcacag
     2821 gtcaggcgcg atgcgaaggc gatcgctatt gtctatgaga accaaaccct cagctacgca
     2881 gaattgaacg cacaagccaa tcaactggca catcggctca gagagctggg cgtgatcccg
     2941 gacagtcgtg tgggcatctg catccaacga tcccccgaga tggtgatagg gctactggcg
     3001 atcctcaaag cgggcgcagc ctatgtgccg ctcgatccag gctatcccag tgagcgcctg
     3061 gccttcatgc taagtgacag cgcgccgctc gcggtgctca cgcacgagcc tgcaggtgct
     3121 gctctgcagg cggcgatggc tgcgctgagc cctcagccgg cactattgaa tctgagtgct
     3181 cccacctggc agcagcactg cagtgaggac ccggaccccc gcagcatcgg cctgctgccg
     3241 actcatctcg cctatgtcat ctatacctcc ggctccacag gtcagcccaa gggcgtgatg
     3301 atcgagcatg agaatctagt gaattatttg gggtgggcat tgacggcata tcgcgtcggc
     3361 caaggcgctg gcgccgtggt gaatacatcc gtttcgttcg attcgacgat aaccagtcta
     3421 tacctaccgc tgctttgcgg ccgaacagta gtactcgtaa gggaggtcga tgaactcgag
     3481 gagctggagc tgcagttgca gcgccgcccg aatttaagtc tggtcaagat ttctcccacc
     3541 catctcaaca cattggggca gcgcttccga gtcgccagac ccaacctatc gcttgcggca
     3601 atcgttgtgg gtggagaaac actattgccc tcgacagtgg aactttggca gagtattgcg
     3661 ccaactattc gcctcatcaa cgagtacggc cctaccgaaa ccgttgtcgg ttgcagctat
     3721 ttcgacatac cggtggattg ggatgcagct gccgcggtgc cgattggtcg gccgattgcc
     3781 aacacgaaga tctatgtgct ggatctgcat cagcaaccgg tgccgatagg tgtcgtcggc
     3841 gaaatctaca ttggcggcag cgggctcgcg cgcggctatt ggaatcgtcc cgagctctcc
     3901 cgcgagcggt ttgtccagga tccgttcaat agcgatccag cggctcggct atacaggacc
     3961 ggagatctgg gccgctggtc agccagcgca aatctggaat tcctgggccg aaacgatcga
     4021 caggtaaaga tacggggcta tcgcatagag ctggaagaga tcgaggcgca attgctgtgc
     4081 cacccgaaga tcgcacaggc ggcagtcctg acgcgtgaag attcactcgg tgaaaagcag
     4141 ctgcttgcct actacatctg caacggcgcc gggcacgctg acgccagcga tctgcgcgag
     4201 cacatgaggt ccgcgctgcc caagtatatg ctacccgtag cgtacgtccc cctggagtca
     4261 ttccccatga ctcccaacgg caagcttgat cgagcggcgc tggctccgcc gggaacaatg
     4321 gcatatggcg taagagaata tgaagccccg gagggcgaag tagagcaagt tctcgccgaa
     4381 atctggctgg acctgctcgc catcgggcgc gtcggccgac tcgatcattt cttcgacctg
     4441 ggtggacatt ctttgctggc cctgcaaatg atttcccgcg ttgaacacag gtttcagata
     4501 gaagtgccca tcagactggt gttcatgtcg cccgagctcg cgcaactggc cgcccgcata
     4561 gacgggttgc gacacgcacg tttgcgcgat cagctcgagc ttggaggcga caccattgag
     4621 gacctactcg agagcgtcag ttcgatgtca gacgccgcgg ctcgggaaat gcttcggcgg
     4681 atcaacctgg gcacaccgca gtgagcggaa tggccaatgc cggtaaccac gcactgtctg
     4741 ccgacaaggc ggcactcctc aactcattgg ttgagcgaag cctcaggcac gagcgcagga
     4801 tcgcgccgct tccgcgcg
//
LOCUS       DATMRM010000133        21386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2849734, whole genome shotgun sequence.
ACCESSION   DATMRM010000133 DATMRM010000000
VERSION     DATMRM010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 21386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21386
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2849734"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VNZ06_03715"
     CDS             <1..244
                     /locus_tag="VNZ06_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW19889.1"
                     /translation="IGRPYLQVADRGRGIESTDAERIFEPFFTKAKRGTGLGLFLARE
                     LAQANGATLLYEAREGGGSIFRIVFADPSRWTGLYS"
     gene            259..1680
                     /locus_tag="VNZ06_03720"
     CDS             259..1680
                     /locus_tag="VNZ06_03720"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWW19890.1"
                     /translation="MSNKPTVLVVDDEPDLIELVSLTLGRMSLSTDSAGDLSGARGKL
                     AERHFDLCLTDMRLPDGDGLDLVAHIQERYPNLPVAVITAHGNIESAVRALKLGAFDF
                     VSKPLDLGVLRKLVDKAIKLADRTSTVSIAQLAVAAAPVQGSRLMGQSRAVASLRELV
                     ARVSRSQAPVHITGESGTGKELVARLIHETGGRHDAPFVPVNCGAIPSELMESELFGH
                     KRGSFTGAITDKRGLVQSAEGGTLFLDEVADLPLHMQVKLLRVIQEKCVRPVGEQREV
                     AVDVRILSATHKNLAELVGHGRFREDLYYRINVIEIRVPALREHAEDIAELADAMLQR
                     MAHRGGVQMPRITAEARSALQSYPFPGNVRELENILERAIALSASGVIDVADLQLRPI
                     TAAPVPASTEAAAAPAGVAETALAAASASAAAPLGDRLETMERDAIVRALEQTRYNKT
                     AAAKLLGMSFRALRYRVKKLKIE"
     gene            1677..2117
                     /locus_tag="VNZ06_03725"
     CDS             1677..2117
                     /locus_tag="VNZ06_03725"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HWW19891.1"
                     /translation="MSSWHLSLLELPQYFLVACCALAVDTGILLLLSGHFHLPYLLAA
                     SCSFLLGGVVGYQLCTRFVWKSDPTASKSYQATLFTLLGVVGLGINAVVMFGMVSGLH
                     TPVLIGKGISAGCTFLCNYWLRRRWVFGRATRRIVSWLPQRAGE"
     gene            2114..3700
                     /locus_tag="VNZ06_03730"
     CDS             2114..3700
                     /locus_tag="VNZ06_03730"
                     /inference="COORDINATES: protein motif:HMM:NF005546.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWW19892.1"
                     /translation="MSRCAVIAGAGPAGLTAALELLGRTDVRPIVIEADSQVGGIAKT
                     IEYQGNRMDLGGHRFFSKSEWVMRWWESILPIAHSADEPTLHLQYQGRSTHYSPGTQA
                     RAAIDRVMLVRKRLSRIFYRRRFFDYPLKLNLQTVRNLGPVYMAQISASYARARLRPR
                     SPETSLEDFLVNRFGDRLYRTFFKDYTEKVWGVPCRDIDAEWGAQRIKGLSISKALKH
                     AMMKPLRQFDTRQKQLETSLIERFLYPKFGPGQMWEEVAARVAARGGEIHLRHRVVNV
                     TREAQTVTAVTVQDVVSGELRPLACDYFISSMPLAELMRYLAPADPRVANTAEHLPYR
                     DFMTAGLLVRRMSPVARAGARQSSNGMPPDNWIYVQESDVKLGRLQVFNNWSPALVAD
                     PSTIWLGLEYFCTQGDELWSMSDGSFLAFAAAELEKIGLISQSDVLDGTVVRVPKAYP
                     AYFGNYRDIGCVREYLDGIGNLLPIGRNGMHRYNNQDHSMLAAKAAVDCIATGSTARA
                     QLWAVNAEDDYLETASSEAA"
     gene            3697..5565
                     /locus_tag="VNZ06_03735"
     CDS             3697..5565
                     /locus_tag="VNZ06_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19893.1"
                     /translation="MSATPQATQAISRSAVAAFALLAAAAWLVLHLYWGIAHDGVLYT
                     MQGLAHLHPDFYRDDVFLRYGSQDRYTVFGELYGRTIGVFGVDHAAALLTLISQLALL
                     WGGWSLARRFNSPSLAWLAVALFAVLDLPYGSGSVFTLMEDFVTPRELSEALVLGAMA
                     AMLADRRWLAIGLLAGSLVLHPLMSLGGILWFVGSSIAANRRGFRLVGTIAVLALPLV
                     ALGAWLLRTPPESRDWAALAIDRAPYLVLTHWTAVAWSNLSRPFVTLATLALLTSDAT
                     TRRLTLAALAVSIAGLLCTLIGGDWLRLMPVLQAQPWRWIWIAVAFSVLMLPAVIAQL
                     WPLGRQGRAALALLLTVYFFQDEIYAPVIAVMAVIAVWVAVRRPALIPPNQQKLMLGG
                     ALLLLAIGVAWDLANRFVYAKIPYVAFADLPRLTLLRRIARGSFFPSLLLMTLWWLTF
                     RLRWIPLTRLLMGVGAVALLVAITLAGVRYSGVAYPSAIYSAFHSWRQLIAPGSEVLW
                     TGNPLKTWIYLERPTFYADQQLSTILFSHTAAEVMQQRFAQLEPFLISEGVVMTGDKA
                     VAQAAEAVTLADVCSHAQVRYVVTRHRFAAQPLSVLPASLPFPYGGLKLFDCKREG"
     gene            5784..6263
                     /locus_tag="VNZ06_03740"
     CDS             5784..6263
                     /locus_tag="VNZ06_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HWW19894.1"
                     /translation="MKTVQKGFTLIELMIVVAIIGILAAIAIPAYQNYTIRSQITEGL
                     SLADGLKTAISEYYANNGSFPATAQLNLPGAITGKYVSGITVTGGGMTITYSGSQANK
                     AIVGQTLGLTPYTDANNDIVWVCGTQVAPTGAVIGVATAGATSAGMSAQYLPTVCHS"
     gene            6460..8202
                     /gene="pilB"
                     /locus_tag="VNZ06_03745"
     CDS             6460..8202
                     /gene="pilB"
                     /locus_tag="VNZ06_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HWW19895.1"
                     /translation="MNDNVSLALQPSRAGLGGLPQRLVQDGVVDESTMHTAMQQSLEK
                     KTSFVTQLVASGGIKARDIAIAASSEYGVPLFDLDALTVDMDAIRAVSDKLLAKHRVL
                     PLFKRGKRLFIGVADPTNTHAIDEIKFQSGMAIEAIVVEDDKLQKRVDKAIEQFEAQM
                     PSLGDDDPVDLEALEVSGGDEEAAGDVARDDVEDAPIVRFVNKVLLDAIRRGASDIHF
                     EPFEKMFRVRFRMDGVLKEIAQPPVQLTGKIAARLKVMSRLDIAERRVPQDGRIKMRL
                     SKTRAIDFRVSSCPTLYGEKIVLRILDSSQAMLGIEALGYEPFQKELYLKHLAKPQGM
                     ILVTGPTGSGKTVSLYTGLNILNREDINISTAEDPAEINLPGVNQVNVNNKVGLTFAS
                     ALRAFLRQDPDVVMVGEVRDLETAEIALKAAQTGHLVLSTLHTNDAPQTLTRLVDMGV
                     KPYAIATSVSLIIAQRLARRLCNQCKVLLDVPAEALRKEGFHERDIATGIKVFGPKGC
                     GSCFDGYKGRVGIYQVMEVSEDIGRIIMTGGNALDISQQAEKEGVWDLRRAGLEKVRA
                     GLTSIEEVNSVTVE"
     gene            8229..9464
                     /locus_tag="VNZ06_03750"
     CDS             8229..9464
                     /locus_tag="VNZ06_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWW19896.1"
                     /translation="MAQSSSAIRSVRKDVAFQWEGMDKKGQRLKGKSVASSEQALKLD
                     LRRQGVVATKIKKQSQSFKAGGKPKAEDIAVFSRQLATMLAAGIPLVQAFEIVGAGHE
                     KPSMQKLILDIKADVEGGTSLHESLARHPLYFDDLFVNLVEAGEQAGALESLLDKIAT
                     YKEKSEAIKKKVKKALFYPAAVLVVAVIVTIILLLFVIPQFEDLFKGFGADLPAFTQM
                     VINLSKFVQHDGIFIAIVLGAGAWTFMYFYKRSRKLREFIDRMSLRIPIIGPILNKAS
                     VARFARTLSTMFAAGVPLVEALESVAGATGNIVYEDAVLKMRDEVATGQRLQRAMENT
                     GQFPNMVIQMIAVGEESGALDSMAGKVAKFYEDDVDAAVDSMSSLLEPLIMAILGVLV
                     GGLVIAMYLPIFKLGSVVG"
     gene            9469..10359
                     /locus_tag="VNZ06_03755"
     CDS             9469..10359
                     /locus_tag="VNZ06_03755"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004710834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HWW19897.1"
                     /translation="MLDLLAGSPGAFITCIGVLGLLVGSFLNVVIHRLPLMLERLWRA
                     ECAELGASTPPPAGEPFNLIVPRSACPHCKAPISTAQNIPIVSYLLLRGRCANCSARI
                     SARYPLVELLTGAASAVTAWQVGFGWPAACAIVVTWFLIALTFIDLDHQLLPDCLTMP
                     LLWLGLVASLTGWSSSAHALPTTPVSAIGGAALGYLSLWSIYHLFRLLTGKEGMGYGD
                     FKLLAGLGAWLGYQMLLPIVLLSACVGAVVGLALIALRRQQRGNPMPFGPFLAIAGWV
                     AMLWGPQLVSRYLGLYAPTG"
     gene            10359..10976
                     /gene="coaE"
                     /locus_tag="VNZ06_03760"
     CDS             10359..10976
                     /gene="coaE"
                     /locus_tag="VNZ06_03760"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWW19898.1"
                     /translation="MSPSTLRVALTGGIASGKSTVSTLFAQRGVPVIDSDVIAREVQA
                     PGTALLEQIFQRFGTDLRLPDGALDRAALRRRVFADPQERQALERLVFPAIRARSEQL
                     ATEVTAPYLIHVIPLLVETHSASRFDRVLVVDCCESLQLRRLLARDACDEAQARAMMA
                     SQASRAQRLEIADDVIVNDAEPGALASEVAALDARYRALAAERRR"
     gene            11036..11839
                     /gene="zapD"
                     /locus_tag="VNZ06_03765"
     CDS             11036..11839
                     /gene="zapD"
                     /locus_tag="VNZ06_03765"
                     /inference="COORDINATES: protein motif:HMM:NF003656.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HWW19899.1"
                     /translation="MTEPAAKSDPVPEAEPSIFEQPLNERMRTFLRLDFLYSQALYHN
                     EKDSPWGTRAAVDSLLDILAILTRGDVRTEVLKELERHLSLLGEYQNHPGIDAGRLRS
                     LMSNLTRLRSELSAVNAAWLQPLRDSEFLAGIKHRTAIPGGTCEFDLPDYFFWLNQPA
                     EARTAAFGRWMILLRPICDAIAELLWLTRQNGRARQEMAAGGVFNVTFDRDNPYQLIR
                     IGLPAGCSLYPEISGSHYRVSVRFLEWRSLDQRPAQTEGDVKFTLICCA"
     gene            11848..12051
                     /locus_tag="VNZ06_03770"
     CDS             11848..12051
                     /locus_tag="VNZ06_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HWW19900.1"
                     /translation="MNAAQHRCPTCGRAIEWSDQFPHRPFCSDRCRLVDLGGWLSGER
                     AIPGDSADLTDDAPSSPSAPRDA"
     gene            complement(12061..13809)
                     /locus_tag="VNZ06_03775"
     CDS             complement(12061..13809)
                     /locus_tag="VNZ06_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWW19901.1"
                     /translation="MQPNVSSSTLSGVPSESPALRPAVVFAVLFLVLLVRLLHLSNAM
                     TSPLTYQVGPDEEYYQQFGRAVAAGIGQYRPEFTFMDPAYGYLLGGLFKLVGFNLFVV
                     YLLQALLDTATAYGILTAGRLLGRPRAGLYGAILYGLTSLAIEFSASVLKEIWVSSFM
                     TWWVVAALALMRGGNRRWGWLLFGAFCGVGFGLRSTLLLMGLVGLLLPLFSQGDAQTP
                     RSARLLNVALVAGGLLIALLPWSLRNHYAYSSLSPMAHNGGIVLSQIYYAGNPNGDMW
                     IPDFVNSSHPNEIWRAYAAEASRRLGRTLSPPEVDRYWRDQALDFMREHPGQVLHDML
                     HKLRYWLADVEIPSSRSDAEERLFSPIVRYLPPPGIWLFAMGIAGLAWLATLDRRWLI
                     VGTPIAVAFFGAIVFLSESRFRFYAASTLALAGGVLIDQLSRHRQQLLHWRVSVFAAL
                     AVVLAATSFILGLGVHTPAIRWDSVAWGYVKMGQLAQASAVVERATADQPDSELLLEV
                     RGYIAGAQKRYADAASELQRAIELRPRSHLAHYNLARVYLQLGDSQRALSEARIADQL
                     NPSPDYDALVKQLSTP"
     gene            13884..15038
                     /locus_tag="VNZ06_03780"
     CDS             13884..15038
                     /locus_tag="VNZ06_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWW19902.1"
                     /translation="MRPQFVTALMPCLNEERTLGTCIEKAQACLRSLGLDGEVVVADN
                     GSSDRSIEIAQRLGARVVHQSTRGYGAALRAGIEAARGDVIVMADADDSYDWLDMGRF
                     IEKIAQGFDLVMGNRFQGGIARNAMPALHRYLGNPVLSTLARVIHRAPVGDFHCGMRA
                     FTKAAYGRMKLRAPGMEFATEMVVNSARAGLRIAEVPTALRRDGRDRPPHLRSFRDGW
                     RHLRFIFTYAPNYLYLGPGAALMLSGLALVAALSRGPITLAGQYVGIHFLALGCLLTL
                     TGFNVINLGILAKVIAHSQMPHLNSRVSDWALQSFTLEGGLIAGVVLFIAGTATDSYI
                     LWEWLRSGSEQEATVHTAFVATLLIVLGLNIVFSSFLLNMFAIERRDRSS"
     gene            complement(15035..16729)
                     /locus_tag="VNZ06_03785"
     CDS             complement(15035..16729)
                     /locus_tag="VNZ06_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19903.1"
                     /translation="MLSNLTVTRSSSVRLSLLAGAVITALIMLWAHSLADWHVDYDLS
                     YIFRYLFSKYDYQAAGLALLVLLVAALAPARLNIRSPLTWIGQHSRLIALLSVVLLCV
                     GVKVVYHDHPLAMDEYAAFFQSQVFAAGHLAGRFPVELMDWLIPRDFQNHFFIVSRNT
                     GEVVSSYLPAFSLLLTPFTWLGIPWLCNPLISGLTVLAIHRLALHVFQDVAIAGAAVL
                     LTIASPVFFADGISYYSMSAHLLANTVFALLLINPTRLRALLAGVVGSIALTLHNPVP
                     HALFAVPWVLWLAGRDNGGRLVGSLILGYLPLCLLLGLGWVRFYAQVASAGSATVSSG
                     GILASWQRITQAFALPSWSVVLARLIGLAKVWVWSVPLLPLLAVIGAVRWHRQPICRL
                     LLQSALVTIVGYLIVPVDQGHGWGYRYFHSAWMALPILGAGALARPISARQASLAEED
                     HDIGVLMLGCAMLTLVVGVGLRAYQMNEFIAQDLTHVHSQYCLDRCVILLKAGGGPYE
                     LDLVQNDPWLRSDTIRMVSASSELNAKMLAYEFPGMHLIYRGRDGEVWTQERESAP"
     gene            16788..17627
                     /locus_tag="VNZ06_03790"
     CDS             16788..17627
                     /locus_tag="VNZ06_03790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW19904.1"
                     /translation="MTLASNPLISIVTVCRNAQATVGDTLASVARQQHDFEIEYLVVD
                     GNSSDSTRSIIDRWAQQLPYLRRIYEPDEGIFDAMNKGLRAATGEYVLFLNADDFLTS
                     ADILARAMSGRRPDSTPLPDLIVGDVAMGRLQTRGIWRHRRAPRRMATHRGTGRHPIH
                     QAQFTRRTLLLSVGGFDTRCRLSADTAQYYDLEREFAPTIRFLSGDVAFMRAGGAANA
                     GLGAVWGGSLEIFRHLRPKCGAIRAARMVLGKTLQALLEVRYGDSGHTRWFTHAQTGN
                     QPE"
     gene            complement(17593..18306)
                     /locus_tag="VNZ06_03795"
     CDS             complement(17593..18306)
                     /locus_tag="VNZ06_03795"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HWW19905.1"
                     /translation="MSPLVKILRVLWFAPYRAALLRYRVFASTEHDQVLSGLALDTVI
                     DIGANRGQFALCIRRLYAQARIFSFEPLQGPSRQFARNFRHDPRARLFNVAISTERGA
                     VAMHVTQWDVSSSLLPIGQAQRDSFPFAHESRQEIVTTAPLTDCLGEADMQGTVLLKL
                     DVQGYELTALKGCGVILDRCQYVYVEVSFVELYIGQALATEIVALLFSKGFELRCVAN
                     LSRGRSVRPIQADFLFARA"
     gene            18446..18859
                     /locus_tag="VNZ06_03800"
     CDS             18446..18859
                     /locus_tag="VNZ06_03800"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HWW19906.1"
                     /translation="MVKRGYELVRYGVVGLLCFALGLALPAGLHEYAQVNYLVAFAVS
                     FVLICVVGYFLNAHFTFAIRKPDLSGATRYTLVNLVSLALNLLALKLLVENAHLWYLG
                     ATVLLAAVNAPIGFVVHRLISYRIAQEGQVTDIAP"
     gene            complement(18840..19700)
                     /locus_tag="VNZ06_03805"
     CDS             complement(18840..19700)
                     /locus_tag="VNZ06_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HWW19907.1"
                     /translation="MISRLTRLLKRPAVYPVVHRLIALPRYLTLYRVFRNWRDTRGLR
                     RWTTADQLRFDFYRQFIRADELVFDIGANVGNRAKIFLRLGARVVAFEPQSPCADLLH
                     RELSGNASFRLVRKALGASIGTAELRIANAHYLSTMSDDWIAATRKSGRFGDAQWEKR
                     QQVAVTTFDEAIREFGAPVFAKIDVEGFEPQVLAGLTGRVASGSLEFAAEALDQAMLC
                     LERLDSLFPCVFQFSEAESMRFCWQSWLSLSEARTALRALVGRDAYAWGDIYFRATDL
                     KTGDPTGRYP"
     gene            complement(19750..20145)
                     /locus_tag="VNZ06_03810"
     CDS             complement(19750..20145)
                     /locus_tag="VNZ06_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19908.1"
                     /translation="MAQDTGLPDIQIDSTGLYREDIFTDRKAGTIRRLVPVTQDGNAD
                     NARAVLYSGQTQLLTPTGVLPLGFEIEASSLEDAISKFPAAVKEALEQAIEEAREYRR
                     ESASRIVVPDVAGGIPGGGAIPGGGKIKL"
     gene            complement(20120..20548)
                     /gene="mutT"
                     /locus_tag="VNZ06_03815"
     CDS             complement(20120..20548)
                     /gene="mutT"
                     /locus_tag="VNZ06_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HWW19909.1"
                     /translation="MTLAVRVVAGVLRDSHGRVLIAQRPEGKHMAGFWEFPGGKIAPG
                     ESGEQALARELTEELGVSLRRCHPLLQLRHDYADRVVELDVFVVDDYRGEPTSREAQA
                     LKWVRASELAGEPLLPADRPIIDALNFDEVSVYGTGHRIA"
     gene            complement(20545..>21386)
                     /locus_tag="VNZ06_03820"
     CDS             complement(20545..>21386)
                     /locus_tag="VNZ06_03820"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HWW19910.1"
                     /translation="ANDQRKVIYQQRTEILAATDLGGSIGEMQREATSSLIDQFVTPN
                     SVPQDWNLDGLAEGFQRSFNVSIDPKFWLKAEPELEESKLRSYVHEAAEKSYAQKVER
                     IGAPIMRYVERDVVLKTLDSHWREHLAAMEYLRQGIHLRGYAQKDYRYEFKREAFELF
                     TAMLGRVKFDTVAMLATVEVRTQEQIEREEAERRERLMRALQAQHAEVQPIIGAPPPE
                     VAPGAAPQGAVGAAMRPAPAAVPEGVAPFVRSDRKVGRNEPCPCGSGRKFKHCHGTLA
                     GTQ"
BASE COUNT         4033 a         6689 c         6606 g         4058 t
ORIGIN      
        1 catcggtcgg ccctatctgc aggtcgccga ccgtggtcga ggcatcgaga gcacggacgc
       61 cgagcgaatc tttgagccct tcttcaccaa agcgaagcgt ggcaccggcc ttggcctgtt
      121 tctggctcgc gaattggcgc aggccaacgg cgcgacgctg ctgtatgagg ctcgggaagg
      181 gggcggcagc atatttcgta tcgtttttgc tgatccaagt cgttggacgg gcctctattc
      241 ataatgataa tgcagtagat gtcgaacaaa cccacggtcc tggtcgtcga tgacgagccc
      301 gatctgatcg agctggtatc cctgaccttg ggccgcatga gtctgagcac cgattcggcc
      361 ggcgatctgt cgggcgcgcg cggcaagctg gccgagcgtc acttcgatct gtgcctgacc
      421 gatatgcgtt tgcccgatgg cgacggcctg gacctggtcg cgcacattca ggagcgctat
      481 cccaatctgc ccgtggcagt cattaccgcg cacggcaata tcgaatccgc ggtgcgcgcc
      541 ctgaagctcg gtgcgttcga ctttgtctcc aagccgctcg atctgggagt gcttcgaaaa
      601 ctggtcgaca aggccatcaa gctcgccgat cgcacttcca ccgtgtcgat tgcgcagctc
      661 gccgttgccg cggcgccggt acagggctcg cgcctgatgg gccaatcgcg cgccgtggcc
      721 tcgctgcgcg aactggtggc acgcgtgagc cgcagccagg ctccggtaca catcaccgga
      781 gagtccggaa ccggcaaaga actggtggcg cggctgatcc acgagaccgg cggacgacac
      841 gacgcaccgt tcgtgccggt caattgcggc gcgatcccgt ccgaactgat ggaaagcgag
      901 ctgttcggac acaagcgagg cagtttcacc ggcgcgatca ccgacaagcg cggcctggtg
      961 caaagcgccg aaggaggaac gctgttcctc gacgaggtgg ccgatctgcc gctgcacatg
     1021 caggtcaaac tgctgcgagt catccaggag aaatgcgtcc gaccggtcgg tgaacagcgc
     1081 gaggtagcgg ttgacgtccg gattctgtcg gccacgcaca agaatctggc ggaactcgtc
     1141 ggtcacggtc gcttccgcga agatctctac taccgcatca acgtgatcga gattcgcgtg
     1201 ccggcgctgc gcgagcacgc cgaggacatc gccgagctgg ccgatgcgat gctgcagcgg
     1261 atggctcatc gcggcggtgt gcagatgcca cgcatcactg ccgaggcacg ctcggcgctg
     1321 cagagctacc cgtttccggg caacgtgcgc gaactggaaa acatcctgga gcgggcgatc
     1381 gctctgtccg cctcgggcgt catcgatgtc gcggacctgc agctgcgacc catcacggcc
     1441 gctccggttc cggcctctac cgaagccgcg gctgcccccg caggggtcgc cgaaacggcg
     1501 cttgccgccg ccagcgccag tgcggccgcc ccgctgggcg atcgactgga aacgatggaa
     1561 cgcgacgcga tcgtccgcgc gctcgagcag acgcgatata acaagaccgc tgcggcgaag
     1621 ctgctgggaa tgagtttccg tgcactgcgt tatcgggtga agaagctgaa gatcgaatga
     1681 gtagctggca tctcagcctg ctcgagctac cgcaatactt tctggtcgcg tgctgcgcgc
     1741 tggccgtgga caccggcata ctgctgctgc tcagcggcca ttttcacctg ccctatctgc
     1801 tggccgcgag ctgctcgttc ctgctgggcg gtgtggttgg atatcagctt tgcacacgct
     1861 tcgtgtggaa atccgacccg acggcatcca aatcctacca agcgacgtta ttcaccctat
     1921 tgggagtcgt cgggctcggg atcaacgcgg tggtcatgtt cggtatggtg agcgggctgc
     1981 acacaccggt gctcatcggc aagggcattt ccgcaggctg cacgttcctg tgcaattact
     2041 ggctgcggcg acgctgggta ttcggacgcg cgacacgccg tatcgtctcc tggctgccgc
     2101 agcgggctgg cgagtgagcc gttgcgccgt catcgcaggc gccggccccg cgggtctcac
     2161 cgccgcactg gaactgctcg gccgcaccga tgtgcgaccg atcgtcattg aggctgacag
     2221 ccaggtcggc ggcattgcca agacgatcga atatcagggc aaccgcatgg acctcggcgg
     2281 ccatcgcttc ttctccaagt ccgaatgggt catgcgctgg tgggagtcga ttctgcccat
     2341 cgcacactcg gcggacgaac cgacgctgca tctgcagtat cagggtcgca gtacccacta
     2401 ttcccccgga acacaggcgc gcgcggccat cgatcgcgtg atgctggtgc gcaagcggtt
     2461 atccaggatt ttctatcgcc gccggttctt cgactacccg ctgaagctca accttcagac
     2521 cgtgcgcaat ctggggccgg tgtacatggc gcagatctcg gccagctatg cccgcgcccg
     2581 gctacggccg cgcagtccgg aaaccagtct cgaggatttt ctggtcaatc gcttcggtga
     2641 tcggctctat cggaccttct tcaaggacta caccgaaaaa gtctggggcg tgccctgtcg
     2701 cgacatcgac gctgaatggg gtgcgcagcg catcaaggga ctgtcgatca gcaaggcgct
     2761 gaagcacgcc atgatgaaac ccctgcggca gttcgacacc cggcaaaagc agctcgagac
     2821 cagtctgatc gagcgcttcc tctatcccaa gttcggcccc ggccagatgt gggaagaggt
     2881 cgcagcgcgt gtcgccgcgc gtggcggcga gattcacctg cgccaccgcg tcgtgaatgt
     2941 gacgcgtgag gcgcaaaccg tcaccgcggt gacggtccag gacgtcgttt ccggcgagct
     3001 gcgtccgctc gcctgcgact acttcatctc gagcatgccg ctcgccgagc tgatgcgcta
     3061 tctggcgccg gccgatccac gcgtcgccaa caccgccgaa cacctcccct atcgggattt
     3121 catgactgcc gggttgctgg tccggcgcat gagcccggtg gcgcgcgccg gggcgcggca
     3181 gtcgtccaat ggcatgccgc cggacaactg gatctatgtt caggagtcgg acgtcaagct
     3241 cggacgcctg caggtgttca acaattggag tccggcgctg gtcgccgacc cgagcacgat
     3301 ctggctgggt ttggagtact tctgcacaca gggcgatgag ctatggtcga tgagcgatgg
     3361 ttcgtttctg gctttcgctg cggcggaact ggaaaagatc ggactcatct cgcaatcgga
     3421 tgtgctcgac ggcacggtgg tccgggtacc aaaggcgtat ccggcgtact ttggcaacta
     3481 ccgcgacatc ggctgcgtac gcgagtatct ggatggaatc ggcaatctgc tgccaatcgg
     3541 gcgcaacggc atgcaccggt acaacaatca ggatcattcg atgctggccg cgaaggcggc
     3601 cgtggactgt atcgcgaccg gcagtaccgc tcgcgcccag ctctgggccg tgaacgccga
     3661 ggatgactat ctggaaacgg cttcatccga agccgcatga gcgcgacgcc gcaggccacg
     3721 caagcgatct ctcgatcggc cgtggcggca ttcgcgctgc tggccgctgc cgcctggctg
     3781 gtgctgcacc tgtactgggg aattgcgcac gatggcgtgc tctatacgat gcagggcctg
     3841 gcgcacctgc accctgactt ctatcgcgac gacgtctttc tgcggtacgg ctcgcaggac
     3901 cgctacaccg tcttcggcga actctacggg cgcacgatcg gtgtgttcgg cgtcgatcat
     3961 gcggcggcac tgctgaccct gatctcgcag ctcgcgctgc tctggggcgg ctggagcctg
     4021 gcgcgtcggt tcaactcgcc gtcgctggct tggctggccg tcgcgctgtt cgcggtattg
     4081 gacctgccgt acggatcagg cagcgtgttc acgctgatgg aagattttgt gacgccgcgc
     4141 gaactatcgg aagcgctggt gctgggggcg atggccgcca tgctggccga tcggcgctgg
     4201 ctcgcgatcg ggctgctggc gggaagtctg gtgcttcatc cactgatgtc cctgggcggg
     4261 atcctatggt tcgttggcag ctccattgcc gccaaccggc gtggctttcg gctcgtgggg
     4321 acgatcgcag tgctggccct gccgctggtc gcgctcggcg cgtggctgct acgaacgccg
     4381 cccgaaagcc gggattgggc tgcgctcgcc atcgatcgcg ccccctatct ggtgttgact
     4441 cactggacgg cggtggcctg gagtaacttg tcacgcccgt tcgtcacgct cgccacgctc
     4501 gccttgctga ccagtgatgc cacgacacgg cggctgactc tcgctgcgct cgcggtcagc
     4561 atcgccggcc tgctctgcac actcatcggt ggagactggc tccgactcat gccggtgctt
     4621 caggcgcagc cctggcggtg gatctggatt gccgtcgcat tcagcgtcct gatgctgcca
     4681 gcggtgatcg cgcaactgtg gccgctcgga cggcagggtc gtgctgcgct ggcactgctg
     4741 ctgacggtgt atttcttcca ggatgagatc tatgcgccag tgatcgccgt gatggcggtc
     4801 atcgcggtat gggttgcggt caggcgcccg gcgctgattc cgccgaacca gcagaaattg
     4861 atgctcggcg gagccctgct tctgctggcg atcggtgtcg cctgggacct cgccaaccgc
     4921 tttgtctacg cgaagatccc gtacgtggca tttgccgatc tgcccagact gacactgctg
     4981 cggcggatcg ctcgcggcag cttcttccct tccttgctgc tgatgacgct ctggtggctg
     5041 acgtttcgtc tgcgatggat accgctcacg cgcctgctca tgggtgtcgg tgcggttgcg
     5101 ctgctcgtcg cgataaccct cgccggcgtc cgatactcag gggttgccta cccgtccgcg
     5161 atctactcgg ccttccattc ctggcggcag ctgatcgcac ccggcagtga ggtgctgtgg
     5221 acgggcaatc ctctcaagac ctggatctat ctggagcgcc cgacctttta tgccgatcag
     5281 caactgtcca ccatcctgtt ctcgcacacg gcggccgagg tcatgcagca acgtttcgcc
     5341 cagctcgaac cgtttctgat cagcgaaggg gtcgtcatga ccggcgataa ggccgtggct
     5401 caggctgccg aagccgtcac attggccgat gtgtgctcgc acgcgcaggt gcgctacgtg
     5461 gtgacacgtc accgatttgc ggcgcaaccg ctgtccgtgc tgccggcatc actgccgttc
     5521 ccctacggtg gattgaagct gtttgactgt aagcgggagg gttagacgac ccgggtggac
     5581 aaatcgtgtc gctttccgac acggcggggc aatcgttgtc gtctggcgcc tgcagcggtt
     5641 ctgtcaaacc gcgatttttc gccaaaaaac tacaactaca gcaagcagtt acgaattcgt
     5701 aagaactggc acgttcattg cttttccaac gaccagcggc tgcgctagcc gcagcctgtt
     5761 taaacttcta ggagctggcg tttatgaaga ctgttcagaa gggtttcaca ctcatcgagt
     5821 tgatgatcgt ggtcgcgatc atcggcatcc tggccgcgat tgcgatcccg gcctatcaga
     5881 actacaccat ccgctcgcag atcactgaag gtctgagtct ggcagacggc ctgaagactg
     5941 cgatttctga gtactatgcg aacaacggct catttccggc cactgcgcag ctgaacctgc
     6001 cgggtgcgat caccggtaag tacgtctcgg gcatcaccgt cactggtggc ggcatgacga
     6061 tcacgtatag cggctctcag gccaacaagg cgatcgtcgg ccagacgctc ggcctgaccc
     6121 cttataccga tgccaacaac gacatcgtct gggtctgcgg cacgcaggtc gccccgaccg
     6181 gtgctgtgat cggcgtcgcg acagcgggtg cgaccagcgc gggcatgtcg gctcagtacc
     6241 tgccgacggt ttgccatagc taataggcag tcgttacgcc ctaaccggtg taactgcaag
     6301 agaagcccct cccatggagg ggcttcttct tttgcgagac cagtcacgta gcgtcctgcg
     6361 gcattgtgta accatggaac gcgattttga cattgcccga ccgggctatc tgagtagact
     6421 aggcggattc cgacaacagg acggggattt cctaacctga tgaatgacaa cgtttcctta
     6481 gcgttgcagc cgtcccgggc agggctgggt ggcctgccac agcgattggt ccaggatgga
     6541 gtggtcgatg agtcgaccat gcatactgcg atgcagcagt cgctggagaa aaagaccagt
     6601 ttcgtgactc agctggtcgc ctccggcggc atcaaggcgc gcgacatcgc gatcgccgcc
     6661 tccagtgagt acggcgtacc gttgtttgat ctcgacgcat tgaccgtgga catggacgcg
     6721 atacgtgcgg tctctgacaa gttgctggcg aagcatcgcg tactgccgct gttcaagcgt
     6781 ggcaagcgat tgttcattgg cgtggccgat cccaccaaca cgcacgccat cgatgagatc
     6841 aagttccagt cgggcatggc gatcgaagcg atcgtcgtcg aggatgacaa acttcagaag
     6901 cgggtcgaca aggccatcga gcagttcgaa gctcagatgc caagcctcgg tgatgacgac
     6961 ccggtcgatc tggaagcgct cgaggtctcc ggaggcgacg aagaagccgc tggcgatgtt
     7021 gcgcgcgatg atgtcgagga cgcgccgatc gtacgattcg tcaacaaggt gttgctcgat
     7081 gcgatccgcc gtggtgcctc ggacattcac ttcgagccct tcgagaagat gttccgcgtt
     7141 cgctttcgga tggatggcgt gctgaaggaa atcgcgcaac cgcccgtgca gctgaccgga
     7201 aagattgccg cgcgactgaa agtcatgtcg cgtctggata tcgcggaacg tcgtgttcct
     7261 caggacgggc gcatcaagat gcgtctgtcg aaaacgcgcg cgatcgattt ccgcgtcagc
     7321 agttgcccga cgctgtacgg cgagaagatc gtgctgcgta tcctggactc ctcgcaggcg
     7381 atgctgggca tcgaggcgct cggctacgag ccgttccaga aagagctgta tctcaaacac
     7441 ctggccaagc cgcaaggcat gatcctggtg accggcccca ccggtagcgg caagaccgtg
     7501 tcgctgtaca ccggcctgaa cattctgaat cgcgaggaca tcaacatctc gacggcggaa
     7561 gacccggcgg aaatcaacct ccccggcgtc aatcaggtca acgtcaacaa caaggtcgga
     7621 ttgaccttcg cgtccgcgct gcgcgcgttc ctgcgccagg acccggacgt ggtcatggtc
     7681 ggtgaggtgc gcgacctgga gaccgcggag atcgctctca aggcagcgca gaccggtcac
     7741 ctggtactgt cgaccctgca taccaatgat gccccgcaaa cgctgacgcg gctggtggac
     7801 atgggtgtga agccgtacgc gattgcgacc tcggtgagtc tgatcatcgc gcaacgactg
     7861 gcgcgccgtc tgtgcaacca gtgcaaggta ttgctcgacg tgccggccga ggcgctgcgc
     7921 aaagagggat tccacgaaag ggacatcgca acgggcatca aggtctttgg gcccaagggt
     7981 tgcggcagct gcttcgacgg ctataagggc cgcgttggca tttatcaggt gatggaagtg
     8041 agcgaagata tcggccgcat catcatgact ggtggcaatg cgctcgatat cagtcagcag
     8101 gccgagaagg aaggcgtctg ggacttgcga cgggccggac tggaaaaagt tcgcgccggg
     8161 ctcaccagta tcgaagaagt caacagcgtc actgtcgaat gagttgcgaa gcacagggaa
     8221 actaatccat ggcgcagtca tcatcagcca tccgatcggt ccgaaaggac gtggccttcc
     8281 agtgggaggg catggacaag aaggggcagc gactcaaggg caagagcgtc gcctcgagcg
     8341 agcaggcact caaactcgac ctgcgtcgtc agggcgtcgt cgcgaccaag atcaagaagc
     8401 agtcgcagtc attcaaggcc ggtggcaagc ccaaggcgga agatattgcg gtgttcagcc
     8461 gccagctcgc tacgatgctg gccgcgggta ttccgctggt gcaggcgttt gagatcgtcg
     8521 gtgccgggca tgaaaagccc tcgatgcaga agctgattct cgacatcaaa gcggacgtcg
     8581 aaggcggcac ctcactgcac gaatcgctgg ccaggcaccc gctgtatttc gacgatctgt
     8641 tcgtcaatct ggtggaagca ggcgaacagg ccggtgcgct ggagagtctg ctcgataaga
     8701 tcgcgaccta caaggaaaaa tccgaagcga tcaagaaaaa ggtgaagaag gcgctgttct
     8761 atccggctgc ggtgctcgtg gtcgcagtga tcgtgacgat catcctgctg ctgttcgtga
     8821 tcccgcagtt cgaggatctg ttcaagggtt tcggcgccga cctgcctgcc ttcacgcaaa
     8881 tggtcatcaa cctgtcgaag ttcgtgcagc atgacggcat cttcatcgcg attgtgctgg
     8941 gcgcaggcgc ttggactttc atgtatttct acaagcgctc gcgcaagctg cgtgaattca
     9001 ttgaccgcat gtcgctgaga attccgatca tcggcccgat cctcaacaag gcctcggtcg
     9061 cacgctttgc ccgaacgctg tccacgatgt tcgccgcagg tgtacccctg gtcgaggcgt
     9121 tggaatcggt cgcgggcgcg accggcaaca tcgtgtacga agacgccgtg ctgaagatgc
     9181 gcgacgaggt ggcaaccggt cagcgtctgc agcgggccat ggagaatacc ggacagtttc
     9241 cgaacatggt cattcagatg atcgcggtcg gtgaagagtc cggcgcgctc gactcaatgg
     9301 ccggcaaggt cgctaaattc tacgaggacg acgtcgacgc ggccgtggac agcatgtcga
     9361 gcctgctcga accgctgatc atggccatcc tgggcgtcct ggtcggtggc ctcgtgatcg
     9421 cgatgtatct gccgatcttc aagctcggtt ccgtcgtcgg ctaacggcgt gctcgatctg
     9481 ctggcgggat cacccggcgc gttcatcacg tgcatcgggg tcttgggtct gctcgtcggc
     9541 agcttcctta atgtggtgat tcaccgactg ccgctcatgc tcgagcggct gtggcgcgca
     9601 gaatgcgccg aactcggcgc cagcacgccg cccccggccg gtgagccatt caatctcatc
     9661 gtgccgcgct cggcgtgccc ccactgcaag gcgccgatca gcactgccca gaatattccg
     9721 atcgtcagtt atctgctgct gcgcggacgc tgtgccaact gctcggcgcg gatcagcgcg
     9781 cgctatccgc tggtggagct gctgaccggc gctgccagtg ccgtgactgc ctggcaggtt
     9841 ggatttggct ggcccgcggc ctgtgcaatc gtcgtcacct ggtttctgat cgcgctcacg
     9901 ttcatcgacc tcgatcatca gctgctgccg gactgcctga cgatgccgct gctgtggctg
     9961 ggactggtgg ccagtctgac gggatggtcc agcagtgcgc atgcgctgcc caccacgccg
    10021 gtcagtgcca tcggcggggc cgcactggga tacctgtcgc tgtggagcat ctatcatctg
    10081 ttccgactgc tcaccggcaa ggaaggcatg ggctacggcg acttcaaact gctggcgggg
    10141 ctcggcgcct ggctcggcta ccagatgctg ctgccgatcg tgctgctgtc ggcctgcgtg
    10201 ggcgccgtcg taggcctggc gctgatcgcc ttgcgacgcc agcaacgcgg caatccgatg
    10261 ccttttggcc cgtttctggc gattgcaggc tgggtcgcga tgctgtgggg cccacagctg
    10321 gtcagtcgct atctggggct gtacgccccg accggctgat gtcgcccagc accctgcgag
    10381 tggccctgac cggcggcatc gccagtggca agagcacggt gtccactctg ttcgctcagc
    10441 gcggcgtgcc ggtgatcgat tccgatgtga tcgcgcgcga ggtccaggcg cccgggacgg
    10501 cgctgctcga gcagatcttc cagcgcttcg gcaccgatct gcggctgccc gacggcgcgc
    10561 tggatcgcgc cgcgctgcgc cgacgtgtgt tcgccgatcc gcaggaacgg caagcgctcg
    10621 agcgcctggt atttccggcc atccgtgccc gcagcgaaca gctcgcgact gaggtcaccg
    10681 cgccctacct catccacgtg attccgctgc tggtggaaac ccactcggct tcgcgcttcg
    10741 accgggttct ggtggttgac tgttgcgagt cgctgcaact gcggcgcttg ctggcccgcg
    10801 acgcctgtga cgaagcccag gcccgcgcga tgatggccag tcaggccagc cgcgcgcagc
    10861 gactggagat agcggacgat gtgatcgtca acgacgcaga accgggggca ctggcctcgg
    10921 aggtggcggc gctggacgcg cgataccgcg ccctggcggc cgaacgacgt cgttagtggc
    10981 cgtcgtcaat cgacgaccgt ttacgcgcgg tgagtgactc aagcacaata acccgatgac
    11041 ggaacccgcg gccaagtccg acccggtccc agaggccgag ccgtcgatat tcgagcagcc
    11101 gctgaacgag cggatgcgca cgttcctacg cctggatttt ctctatagcc aggcgctgta
    11161 tcacaacgag aaagacagcc cttggggcac tcgcgctgcg gtggacagtc tgctcgacat
    11221 tctggcgatt ctgacccggg gcgacgtgcg cacggaagtg ctcaaggaac tcgagcgcca
    11281 tttgtcgctg ctgggggagt atcagaatca ccccggcatc gacgccgggc gactgcgctc
    11341 gctgatgtcg aacctgacac gactgcgctc ggaactcagt gccgtcaatg ccgcgtggct
    11401 gcagccgctg cgggactccg agtttctggc gggtatcaag caccgcaccg ctattccggg
    11461 cggaacctgc gagttcgatc tgccggatta tttcttttgg ctcaaccagc cggccgaagc
    11521 gcgcaccgcg gccttcggtc gctggatgat tctgttgcgc ccgatctgtg atgccatcgc
    11581 ggagttgctg tggctcacgc ggcaaaacgg cagggctcgc caggaaatgg ccgctggcgg
    11641 cgtgttcaac gtcaccttcg atcgcgacaa tccgtatcag ctcattcgca tcggattgcc
    11701 ggccggctgc tcgctctacc cggagatcag cggcagtcac taccgcgtca gcgttcggtt
    11761 cctggaatgg cgcagcctcg atcagcggcc ggcccaaacc gaaggcgacg tcaagttcac
    11821 gctgatctgc tgtgcctgag ggtcaccgtg aacgctgcgc agcaccgttg tccgacttgc
    11881 ggccgagcga ttgaatggtc cgaccagttt ccgcatcgcc cgttttgcag cgatcgctgc
    11941 cgattggtgg atctgggagg ctggctcagc ggcgagcgcg ccattccggg tgattcggcc
    12001 gatctcactg acgacgcccc gtcgtcgccc agcgctccgc gcgacgcctg atcgaacgcc
    12061 tcagggcgta gagagctgct tcaccaaggc atcatagtcg ggtgaggggt tgagctggtc
    12121 ggcgattctc gcctcactca gcgcgcgctg cgaatcaccc agctgcagat agacccgcgc
    12181 caggttgtag tgcgccaggt gcgatcgagg ccgcagctcg attgcgcgct gcaattcgct
    12241 ggcagcatcg gcgtagcgct tctgcgctcc cgcgatgtat ccccgcactt cgagcagcag
    12301 ctcgctatca ggctgatcgg cggtagcgcg ctctacaacc gcactcgcct gcgcgagctg
    12361 gcccatcttc acatagcccc aggccaccga gtcccatcta atcgcgggcg tgtgaacgcc
    12421 gagcccgaga atgaacgagg tcgcggccag gacgaccgca agagcggcaa ataccgaaac
    12481 gcgccagtgc aacagctgct gccggtgacg cgacagctga tcgatgagca cgccgccagc
    12541 caaggccagc gtggaggccg cgtagaatct gaagcgcgat tcggaaagga aaacgattgc
    12601 cccgaaaaat gccaccgcga tcggtgtgcc aacgatgagc cagcgccgat ccagcgtggc
    12661 gagccatgcg agaccggcga tgcccatcgc gaacagccag atccccggcg gtggcagata
    12721 gcgcactatg ggtgagaaca aacgctcctc cgcgtccgag cgggaactgg gtatttcgac
    12781 gtctgccagc caatagcgca gcttgtgcag catgtcgtgc agcacctgcc ccgggtgctc
    12841 acgcatgaag tccagggcct gatcgcgcca gtagcggtcc acctcgggcg gtgacagcgt
    12901 gcggccgagc cgccgactgg cctcagccgc gtaggcccgc cagatctcgt tcggatgtga
    12961 ggaattgacg aagtcaggaa tccacatgtc gccgttggga ttgccggcat agtagatttg
    13021 cgagagtacg attccaccgt tgtgcgccat cggcgagagg ctggagtagg cgtaatggtt
    13081 gcgcaggctc cagggcagca gcgcgatcag cagcccaccg gccaccagcg ccacattgag
    13141 caggcgcgcg cttcgtggcg tctgcgcgtc gccttggcta aacaaaggca gcaacagtcc
    13201 gaccaggccc atcagcagca gggttgagcg caaaccgaag cccacgccac agaatgcacc
    13261 gaacagcagc cagccccaac ggcgattccc gccccgcatc agcgccaagg ccgccaccac
    13321 ccaccaggtc atgaagctgc tgacccagat ctccttcaac acgctggcgg agaactcgat
    13381 ggccagactc gtcaggccgt agagtatggc gccatacaaa cctgcgcgcg gacgccccag
    13441 cagtcggccg gccgtcagga tgccgtacgc ggttgcggtg tccagcagag cctgcaacag
    13501 atagaccacg aaaagattga agccaactag cttgaacagt ccaccgagca gatagccgta
    13561 ggcgggatcc atgaacgtga actcgggcct gtattggccg atacctgcag ccaccgcgcg
    13621 cccgaattgc tggtagtact cctcgtccgg cccgacctga taggtgagcg gactggtcat
    13681 cgcattcgac agatgcagca gtcgcaccag cagaaccaga aaaagcaccg caaacaccac
    13741 ggcgggccgc aatgccggcg actcgctcgg aacgccggat agtgtgctgc tgctcacatt
    13801 cggctgcatc acgtggttgg caagtgcact gatcgaaaat gcataatgaa agtatagccg
    13861 ccccaccccc gccaatcccc acgatgcgtc ctcaattcgt cactgcgctg atgccttgtc
    13921 tcaacgaaga gcgcacgttg ggcacctgta ttgagaaggc acaggcctgc ctgcgatccc
    13981 tggggctgga cggtgaggtc gtggttgctg acaacggctc ctcggatcgc tcgatcgaga
    14041 ttgctcaacg actcggtgcg cgcgtcgtac accaaagcac ccgtggctac ggcgctgcat
    14101 tgcgggccgg catcgaagcc gcgcgcggcg atgtgatcgt gatggccgat gcggatgatt
    14161 cctacgactg gctggacatg ggccgattca tcgagaaaat cgcacaggga ttcgatctgg
    14221 tgatgggtaa tcgattccag ggcggcatcg cccgcaatgc catgccggcg ttgcatcgct
    14281 atctgggaaa cccagtgctg tccacgctcg cgcgagtgat ccaccgcgca ccggtcgggg
    14341 atttccattg cggcatgcgc gcgttcacca aggcagccta cgggcgcatg aaattgcgcg
    14401 cgcccgggat ggagttcgcg acagaaatgg tcgtcaactc ggcccgcgcc gggctgcgca
    14461 tcgccgaggt acctaccgct ctacgccgcg atgggcgcga tcggccgccg cacctgcgct
    14521 cgtttcgcga tggctggcgc cacctgcgat tcatcttcac ttacgccccc aactacctct
    14581 atctcgggcc gggggccgcg ctgatgctgt ccggcctggc attggtcgcg gcgttgtcgc
    14641 gcggaccgat cacactggcc ggacagtacg tcggcatcca ttttctggca cttggatgtc
    14701 tgctgacact tacgggcttc aatgtcatca atctcggcat cctcgcgaaa gtgatcgcgc
    14761 actcacagat gcctcatctc aattcgcggg tgagcgactg ggcgctgcag agcttcacgc
    14821 tcgagggagg gttgatcgcc ggcgtcgtgc tgttcatcgc cggcaccgcc accgattcgt
    14881 acattctctg ggagtggctc aggagcggta gcgagcagga ggccacggtc cacactgctt
    14941 tcgtcgcgac gctgctgatc gtgctcggac tgaatatcgt gttcagctcg ttcctgctga
    15001 acatgttcgc gatcgaaagg cgggaccgat cttcctaggg cgcagactcc cgctcctgcg
    15061 tccagacttc gccgtcccga ccgcggtaaa tcaggtgcat gcccggaaat tcgtacgcca
    15121 acatcttggc attgagttcg gaagaagcgc tcaccattcg aatcgtgtcg ctgcgtagcc
    15181 acggatcgtt ctgtaccaga tccaactcat agggacctcc tcctgccttc agcaagatga
    15241 cgcaccggtc caggcagtac tggctatgca cgtgcgtgag atcctgggcg atgaattcgt
    15301 tcatctggta agcgcgcaac ccgaccccga caaccagcgt cagcatcgcg cagccaagca
    15361 tcaatacccc gatatcatga tcttcctcgg ccagtgacgc ttgcctggca cttatcgggc
    15421 gagccagcgc ccccgcgccc agaatcggca aagccatcca ggccgaatgg aagtaccgat
    15481 acccccagcc gtgcccctga tcaacgggaa cgatcagata cccaacgatc gtcaccagcg
    15541 ccgactgcag cagcaagcgg cagatcggct gtctatgcca gcgaaccgcg ccgatcaccg
    15601 ccagcagagg cagcaacggg actgaccaga cccagacctt tgccaggccg atcaggcgcg
    15661 ccagcaccac cgaccacgag ggtagcgcaa aggcttgcgt gattcgttgc cagctggcca
    15721 ggatgccgcc ggaagagacc gtggcagacc ccgcgctcgc gacctgcgca tagaaccgca
    15781 cccagcccag ccccagcagc aggcacagcg gcagatagcc gaggatcaga gatcccacca
    15841 gccgcccgcc gttgtcgcgt ccggccagcc acaagaccca cggcacagcg aacagcgcat
    15901 gtggcaccgg gttgtgcagc gtcagcgcga ttgaacctac gactcctgca agcaaggccc
    15961 gcagccgcgt cgggttgatg agcaacagag cgaagacggt gttggcgagc agatgcgctg
    16021 acatcgagta gtaggagatg ccatctgcaa aaaacaccgg cgaggcaatc gtcaacagta
    16081 cggcggcacc ggcaatggcg acgtcctgga acacgtgtag cgccagacga tgtatcgcga
    16141 gcaccgtcaa cccggagatc agcgggttgc acaaccacgg gatgccaagc caggtaaacg
    16201 gtgtaagcag caacgagaat gcgggcaggt acgatgagac gacttcgccg gtattcctgg
    16261 agacaatgaa gaaatggttc tggaagtccc ggggaatcag ccagtccatc agttccactg
    16321 gaaaccggcc tgccagatga ccggcagcga atacctggct ttggaagaac gccgcatatt
    16381 catccattgc gagcgggtga tcgtgataga cgactttgac acccacgcac aacagcacga
    16441 cgctcagcag cgcaatcagc cgtgaatgct gcccgatcca ggtcagtggc gatcggatgt
    16501 tgagccgcgc cggcgccagc gctgccacga gcagtaccag cagagcaagc cccgcggcct
    16561 gatagtcata cttggagaac agatagcgga agatatagga cagatcgtag tcgacgtgcc
    16621 agtctgcaag actgtgcgcc cagagcataa tgagcgcggt gatcacagcg cctgccagca
    16681 acgacaaccg cacggaggat gagcgcgtca cggtcagatt cgaaagcacc ccactcaact
    16741 cctaagatgg ctggtccagc ggcttgttgc taggatcatt ctgacagatg acgctcgcct
    16801 ccaacccgct catttccatc gtcacggttt gcaggaatgc gcaggcgacc gtaggcgata
    16861 ccctggcctc ggtggcacgg cagcagcacg attttgagat cgagtacctg gtggtcgatg
    16921 gcaactcgag cgattcgacc cgttccatca tcgaccgctg ggcgcaacag ctaccgtatc
    16981 tgcggcgcat ctatgagccg gacgagggca tcttcgacgc gatgaacaag ggactgcgcg
    17041 ccgcaactgg cgagtatgtg ctgttcctca acgccgatga cttcctgacc tcagcggaca
    17101 tactcgcccg cgcgatgagc ggcagacggc cggatagcac tcccctgccg gatctgatcg
    17161 tcggcgacgt cgcgatggga cgcttgcaaa cacgtggtat ctggagacac cgtcgcgccc
    17221 cgcgcaggat ggcgacccac cgtggtaccg gccggcatcc gatccaccag gcacaattca
    17281 cccggcggac ccttctgctg agtgtcggcg gcttcgacac ccgctgtcgt ttgtcggccg
    17341 acacggccca gtattacgac ctggaacgcg agtttgcacc gaccattcgc tttctctcgg
    17401 gcgacgtcgc cttcatgcgg gccggcggtg ctgctaacgc gggtctaggc gccgtctggg
    17461 gcggtagcct ggagatcttc cggcatctgc ggccaaagtg cggcgcgatc agggccgcgc
    17521 gcatggtgct cggcaagacg ctgcaggctc tgcttgaagt acgctatggc gacagcggac
    17581 atacgcgctg gttcacgcac gcgcaaacag gaaatcagcc tgaataggcc gtaccgatct
    17641 accgcgcgac agattggcga cacaccgaag ttcaaatccc ttggagaaca gcagcgccac
    17701 aatctcggta gcgagcgcct gtccgatata gagctccacg aaggacactt cgacatatac
    17761 gtactggcat cggtcgagga tcaccccaca gcccttcaac gcggtcagct cgtagccctg
    17821 cacgtcgagc ttcagcagca ccgtaccctg catatcggcc tcgcctaagc aatcagtgag
    17881 aggcgccgta gtcacgatct cctggcgcga ctcatgggcg aacggaaagc tgtcgcgctg
    17941 ggcttgcccg atgggtagca aagacgagga tacatcccat tgggtgacat gcattgcaac
    18001 cgccccgcgc tcagtcgaga tcgcgacgtt gaagagccga gcgcgagggt catgccggaa
    18061 gttgcgcgcg aactgacgtg agggtccctg cagcggttcg aacgagaaga tccgggcctg
    18121 cgcatatagc ctgcgaatgc agagcgcaaa ctggccgcga ttggcgccga tgtctatcac
    18181 cgtgtcgagc gccaatcccg acaggacctg gtcgtgttcc gtagacgcga acaccctgta
    18241 ccgcagcagg gcagccctgt aaggcgcgaa ccacagcacc ctcagaatct tgaccaatgg
    18301 gctcacgatt gggctggcct cgtcctcatg gcttcatcat ccgccacaca taaccgagct
    18361 attgagaccg tcatcacacc ggcgctctgg ccgccagcgc accgccattg tagcgtgtcg
    18421 gctttggctg ccttaagcgt gagtcatggt taaacgcggc tatgagcttg tccgatatgg
    18481 cgtggtgggt ctgctttgct ttgctttggg gttggcgcta ccggcaggtc tgcatgaata
    18541 cgcccaagtc aactacctgg ttgcgttcgc tgtgtctttc gtcctcatct gcgtcgtcgg
    18601 atacttcctc aatgcccact ttacgttcgc tatccgcaaa cctgacctat cgggtgccac
    18661 gcgctacacg ctcgtcaatt tggtatcgct tgctctcaac ttgctggcgc tgaagctgct
    18721 ggtagagaat gcgcatctct ggtatctggg cgctacggta ctactcgccg ccgttaatgc
    18781 tccgattggt tttgtcgtcc atcggctcat ttcataccgg atcgcgcaag aaggccaagt
    18841 cacggatatc gccccgtagg gtcgccggtt ttcaagtccg tggctcgaaa ataaatgtcg
    18901 ccccaggcgt atgcgtctcg gccgacgagt gccctcaatg ctgttcgcgc ttcagatagg
    18961 ctaagccagg attgccagca gaatcgcatc gattccgcct cagagaattg aaaaacgcac
    19021 gggaaaagac tatcaagtcg ctccagacag agcatcgcct gatccagtgc ctcagctgcg
    19081 aattcgagcg agccggacgc cacgcgcccc gtcaggccag ccagcacctg cggttcaaat
    19141 ccctcgacat cgatcttggc aaatactggc gcgccgaact ctcggatagc ctcgtcaaaa
    19201 gtagtgaccg cgacctgttg tctcttctcc cattgggcgt cgccaaatcg acccgacttg
    19261 cgtgtggccg caatccaatc atcggacatc gttgagagat agtgcgcgtt ggcgattcga
    19321 agctcagcag tcccaatcga ggcgccaagc gccttgcgca ccagccggaa agacgcgttg
    19381 cccgacagct cacgatgcaa caggtctgcg cacggcgatt gcggttcaaa tgcgaccacg
    19441 cgcgcaccta gacgcaagaa tattttggcg cgattcccga cattggcacc aatatcgaac
    19501 acgagctcgt ctgcgcgtat gaactgacgg tagaagtcga atcgcaattg atcggcagtg
    19561 gtccagcggc gtagccccct ggtgtcgcgc caattcctga agacacgata cagggtcagg
    19621 tagcgcggaa gtgcgatgag ccgatgaacg acgggataga cggcaggcct tttcaacaaa
    19681 cgcgtgagtc gtgagatcat cgcactatcg ggttgccggc gatgcagcga gtttgtatcg
    19741 cctgggggct tatagcttga tctttccccc gccgggaatg gcaccaccac ccggaatgcc
    19801 tccggccaca tccggcacta cgatgcgcga agccgactcg cgtcgatact ctcgcgcctc
    19861 ctcgatcgcc tgctcgagcg cctctttgac cgctgccggg aacttgctga tcgcatcctc
    19921 gagcgaactg gcctcgatct cgaacccgag aggcagtacg ccggtgggtg tgagcaactg
    19981 cgtttgaccc gagtagagca cggcacgtgc gttgtcggca ttgccatcct gcgtcaccgg
    20041 taccaggcgg cgaatggtgc cggcttttcg gtcagtgaaa atgtcttcgc gatacagccc
    20101 ggtgctgtcg atctgaatgt caggcaatcc ggtgtcctgt gccatatacg cttacctcat
    20161 cgaaattgag tgcatcaata atcgggcgat ccgcgggcag cagcggctca ccggccagct
    20221 ccgacgccct cacccacttg agcgcctgag cttcccggct ggtgggctcg ccgcgatagt
    20281 cgtctaccac gaagacgtcg agttcgacca cgcgatcggc gtaatcatgc cgcagttgca
    20341 gcagcggatg acagcgccgg agcgatactc cgagctcctc ggtcagttca cgagccaatg
    20401 cctgttcccc gctctcaccg ggagcaatct taccgcctgg aaattcccag aatccggcca
    20461 tgtgcttgcc ctcgggacgc tgagcaatca ggactcgccc gtgggaatcg cgcagcacgc
    20521 ccgctacgac gcgcaccgca agcgtcattg agtgcccgct agcgtaccgt ggcagtgctt
    20581 gaacttcctg cccgaaccgc atggacaagg ctcgttgcgc ccgactttgc ggtcggagcg
    20641 cacgaacggt gccacccctt ccggcaccgc tgccggcgcc ggacgcatgg cggcaccgac
    20701 cgcgccctgc ggcgccgctc ccggcgcgac ttccggcggc ggcgcgccga tgatcggctg
    20761 cacttccgcg tgctgggcct gcagcgcccg catcaggcgt tcgcgccgct cggcttcctc
    20821 gcgctcgatc tgctcctggg tacgcacttc gaccgtcgcc agcatcgcaa cggtgtcgaa
    20881 ctttacccgg cccagcatcg ctgtgaacag ctcgaacgcc tcgcgtttga actcatagcg
    20941 atagtccttc tgggcgtagc cacgcagatg gatgccctgt ctcagatact ccatggccgc
    21001 cagatgctcg cgccagtggc tatcgagtgt cttgagtacc acgtcgcgct cgacgtagcg
    21061 catgatcggc gcgccgatgc gttccacctt ctgggcgtag ctcttctcgg ccgcctcgtg
    21121 cacgtacgat cgcagtttgg attcttcgag ttccggttcc gccttcagcc agaacttcgg
    21181 gtcgatgctt acgttgaagc tgcgctggaa gccttcggcc aggccatcca gattccagtc
    21241 ctgcggcacg ctgttgggcg tcacgaactg atcaatcagc gagctggtcg cttcccgctg
    21301 catttcaccg atcgagccgc ctaagtcggt cgcggcgaga atctcggtgc gctgctgata
    21361 gatgaccttg cgctgatcgt tggcga
//
LOCUS       DATMRM010000134         4692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2849876, whole genome shotgun sequence.
ACCESSION   DATMRM010000134 DATMRM010000000
VERSION     DATMRM010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2849876"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VNZ06_03825"
     CDS             complement(<1..641)
                     /locus_tag="VNZ06_03825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW19911.1"
                     /translation="MSESAARGYAHVGPIAIVSHSHPSLTRGGAEIAAYTLFTGLTRL
                     GCDVTFIAACPENSRSGLSLASTRERALFTDPNAFDSFHHLATPTMVKQFLSVLPRHT
                     RVVNFHHFIHFGLGVLREVGSNLGAVCVLTLHELLSICHHHGQMITRGANALCTQGTP
                     SACSQCFPEHTRQEFALRRQAFLEAYRCLDGFISPSRFLLERYVSWGLDRARL"
     gene            complement(638..2665)
                     /locus_tag="VNZ06_03830"
     CDS             complement(638..2665)
                     /locus_tag="VNZ06_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19912.1"
                     /translation="MNARIDTEQRFQLVGSAPPPRKRVSASLVPADSTLRWRGNIDAV
                     SLEHGIEGWVLDLAAPQTLLRLQLLAGDELIAEGATGVSRDDVVSLLGSSASPGFRFS
                     AEILAELDPKLAELPLCVTIAGENVEIVAGATLPSVAQLMSGELAQPIGFDLMDRLGL
                     LRQGGVTLSASPLRPGPWKEVGCIEVLSIDEGGLVWVQGWMRRCAAIDAPAAVVDGRK
                     EPAGFVYTWFERADLNAEHCGFIGLIHSSWVPTPATAPLLFIKGEEVVFVRGLAPTRI
                     IRHAEFAATFRNLANRCHTGPTAELLQLIDSPSSWTPTQSGASAALDTAVVIPGFGCV
                     AQGWAVNPLSRAVRFALRFGPNILSADEDSIIYSPRPDLSNVAPGCDALLQQAGFVVA
                     FRGEVPVRALDSPLLKVVYEDGSSSRHALETRIFRTIGSAVEPDALLTHYPSLPRERF
                     FPELAFALRKADRMQAASWRPIAITPCLRAIVCVLPPGRSGAYLLAEQLRAHVSVREG
                     AGVLLIAGQTTMRSDAITLRASLAEDAGIPCSLIAARRCAQALYALDSMLKAIGCERF
                     VFLGPGMLPTGSGWDAALEYLGSAERGGCFFGVTDPTSEEPDSTDCAAAIGWTRSALE
                     EWLPTALPLLGGHAKDNGLGQLGFRRIPRSVCYSRELDGSAFVQAVNRVTR"
     gene            complement(2662..3948)
                     /locus_tag="VNZ06_03835"
     CDS             complement(2662..3948)
                     /locus_tag="VNZ06_03835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW19913.1"
                     /translation="MPTQRLKVPSFAQAKQAVRVLIASHGHPEFSRGGAEIAAHELFT
                     ALRQRHGFESWFLGCSDPAHHHSAAAISQPFSGREYLYNIRDFDLFKLSNRDARFPGA
                     FRSLLRELNPRVVHFHHYVNLGVEAFLHVRETLPNSKIVLTLHEYLALCHHSGQMVTR
                     PHLTLCHESSPLGCTRCFHDLTAADFFMRKLYVSRFFELVDLFVAPSRFLSERYVAWG
                     VPQQRMAVIENLTCGRPERPARAPVSSVDSSVLRVGYFGRISPFKGIGVLMEAAKILQ
                     ERGAENIVFEIHGDYSSQPAEVQDEFLDRLKTLGRTVKFCGPYDPMRVDELMQSVQLV
                     LMPSIWWENSPIVIQEAMRNRRPVVCSNIGGMAEKVRDSIDGFHFPVGDALALAALLQ
                     RLAQSPERLAAMTETMAAPNSTVVERHVELYESLAA"
     gene            complement(3948..>4692)
                     /locus_tag="VNZ06_03840"
     CDS             complement(3948..>4692)
                     /locus_tag="VNZ06_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWW19914.1"
                     /translation="QLALFSRRWNPKQIDLMYVLDDVRWRRNAQTLAQSAYERFGIPF
                     RLVLLPSPVGPGPAFNAGLRAARGQYVCFMRPEVLPVNEQWLERLLDRLERHANVGLL
                     SARLLSEDGSIRHEGYRYRPMREFGNWQYPDHLNEGRRPEPKIGLHRCDAVAHGCMLM
                     RRSLALKLGGFDESYLAGEFEDSDLCLKVRARELTCAVDDEVELYQLQSPRHSASTDP
                     RSINLTLYNAWVHQRRWFSASARTLAVAP"
BASE COUNT          975 a         1525 c         1421 g          771 t
ORIGIN      
        1 ttcagacgtg cccgatccag cccccacgag acatagcgct cgagcagaaa gcggctcggt
       61 gagatgaaac cgtccagaca ccgatacgcc tcgaggaatg cctgacgccg cagtgcaaac
      121 tcctggcgcg tatgctccgg aaagcattgc gaacaggcgc tgggtgtgcc ctgtgtacac
      181 agcgcgttcg cgccgcgcgt gatcatctga ccgtggtgat gacagatgct gagcaattcg
      241 tgcagcgtca gcacacagac cgcaccgagg tttgaaccca cttcgcgcag cacaccgagc
      301 ccgaagtgga tgaaatgatg aaaattcacc acacgcgtgt gacgcgggag caccgacagg
      361 aactgtttga ccatcgttgg cgtcgccaga tggtggaacg agtcgaaggc gttaggatct
      421 gtgaataagg cccgctcgcg ggtagaggcc agcgacagtc cggaccgact attctcaggg
      481 cacgcagcga tgaacgtcac atcgcatccg agacgcgtca gcccggtaaa cagcgtatag
      541 gcggcgatct ctgcgccgcc tctggtcagc gaaggatggc tatgactgac gatggcgatc
      601 ggacccacat gagcatagcc gcgtgctgct gactcgctca tcgcgtgacc cggttgaccg
      661 cctgcacgaa cgcggagcca tcgagctcac ggctgtagca cacgctgcgc ggtatccggc
      721 gaaatcccaa ctgcccgagc ccgttgtcct tcgcatggcc ccccagcagc ggcagcgccg
      781 tgggcagcca ctcctcgagt gccgagcgcg tccagccaat cgcagcggcg caatccgtgc
      841 tgtccggctc ctcgctcgtc ggatcggtaa ctccgaagaa gcagccgccg cgctctgcgg
      901 acccgaggta ctccaaggcc gcgtcccatc cagagcccgt cggcagcatt ccggggccca
      961 gaaacacgaa gcgctcgcag ccgatggcct tcaacatcga gtccagcgca tacaacgcct
     1021 gcgcgcagcg gcgggccgcg atcaggctgc acggtatccc ggcatcttca gccagcgagg
     1081 ctcgcagcgt gatggcatcg gatcgcatgg ttgtctggcc ggctatcagc aagactcccg
     1141 caccctcgcg aacgctcacg tgtgcccgca attgctccgc caacaggtat gcgcccgagc
     1201 gacccggcgg caacacacaa acgatcgcgc gcaggcacgg agtgatcgca atcggacgcc
     1261 acgacgcggc ctgcatccgg tccgctttgc gcagcgcaaa ggcgagctcc ggaaagaatc
     1321 gctctcgcgg caacgatggg tagtgggtca acagtgcatc cggctctacg gcgctaccga
     1381 tcgttcgaaa gattcgcgtt tcgagcgcgt gacggctgct cgagccatct tcataaacga
     1441 ctttcaatag cggcgaatcc agtgcgcgca ccggaacctc gccgcggaac gcgaccacga
     1501 agccggcctg ctgcaacaac gcgtcgcaac ctggggccac attgctcagg tccgggcgcg
     1561 gcgaataaat gatgctgtcc tcatccgcgc tgagaatgtt cggaccaaag cgcaacgcga
     1621 agcgcacggc tctgctgagc ggattgaccg cccaaccttg agcaacgcag ccgaaccccg
     1681 ggatgacgac cgcagtgtcg agcgccgcag acgcaccgct ctgagtaggg gtccagctcg
     1741 atggactgtc gatgagctgc agcagctcgg ccgtcggacc cgtatgacag cgattcgcca
     1801 ggttgcgaaa cgtggccgcg aattcggcat gccggatgat tcgggtcggc gccaggccgc
     1861 gcacgaacac cacttcctcg cccttaatga acagcaacgg cgcggtggca ggggttggaa
     1921 cccatgacga gtggatcagg ccgatgaacc cgcagtgttc cgcattcaga tcggcgcgtt
     1981 caaaccacgt atagacgaaa cccgcgggct ctttgcgacc gtctaccacc gcggcgggcg
     2041 catcaatcgc cgcacagcgg cgcatccacc cctgtaccca aaccagccca ccttcgtcga
     2101 tcgacaacac ttcaatgcag ccgacttcct tccatggacc cgggcgcagc ggcgacgcgc
     2161 ttaaggtcac cccgccctga cgcagcaggc ccaatcggtc catgaggtcg aagccgatgg
     2221 gctgcgccag ctcgccgctc atcagttgcg cgacgctggg cagggtcgcg cccgcgacga
     2281 tctcgacgtt ctcgccggca atcgtcacgc atagcggcag ctccgccagc ttggggtcga
     2341 gctccgccag tatctcggcc gaaaaacgaa agcccggcga agcgctgctc ccgagcaggg
     2401 agaccacgtc atcgcggctc acaccggttg cgccttcggc gatcaattca tcgcccgcaa
     2461 gtagctgcag ccgcaacagc gtctgcggag cggcgagatc cagcacccag ccctcgatcc
     2521 catgctcgag gctgactgcg tcgatgttcc cccgccatcg cagcgttgaa tccgccggca
     2581 ccagcgacgc ggacacccgc ttgcgcggtg gcggcgccga gccgacaagc tggaacctct
     2641 gttcggtgtc aatgcgggcg ttcatgcggc gagcgactcg tacagttcga catgccgctc
     2701 gaccaccgtc gagttcggag ctgccatcgt ctcagtcatc gccgccagtc gctccggcga
     2761 ttgggcgagc cgctgcagca gcgccgccag cgccagcgca tcgccgaccg ggaaatgaaa
     2821 accatcgatg ctgtcgcgca ctttttctgc cataccgccg atgttggagc acaccaccgg
     2881 ccggcggttg cgcatcgcct cctggatgac gatcggcgag ttctcccacc agatgctcgg
     2941 catgagcacc agctgtaccg actgcatcaa ttcgtccact cgcatgggat cgtaggggcc
     3001 acagaacttg acggtgcgcc ccagggtctt gagccgatcc agaaactcgt cctgaacttc
     3061 ggcgggctgc gaactgtagt cgccgtgaat ctcgaacacg atgttttcgg caccgcgctc
     3121 ctgaagaatc ttggccgcct ccatcagtac gccgatgcct ttgaatggcg agatgcggcc
     3181 aaaatagccg acccgcagca ccgacgaatc cacggagctg accggcgcgc gcgctggacg
     3241 ttctggcctg ccgcaggtca gattctcgat cacggccatg cgctgctgag gcacacccca
     3301 ggcgacatag cgctccgaca ggaatcggct gggggcgaca aacagatcga ccagttcaaa
     3361 gaaccggctg acatacagct tacgcatgaa gaaatccgcc gccgtcaggt cgtgaaaaca
     3421 gcgggtacag ccgagtgggc tcgactcatg gcacagcgtc agatgcggac gggtcaccat
     3481 ctgacccgag tggtgacaca gcgccagata ctcgtgcagt gtcagcacga tcttgctatt
     3541 gggcagcgtc tcacgcacgt gcaggaacgc ttccacgccg agattcacgt agtggtgaaa
     3601 atgcactacc cgtgggttga gctctcgtag cagcgatcga aaggctccgg gaaatcgagc
     3661 gtcgcgattc gacaacttga acagatcgaa gtcgcggatg ttgtagagat actcccgtcc
     3721 agagaacggc tggctgatgg ccgccgcgga gtgatggtgc gccggatccg aacatcccag
     3781 aaaccatgat tcgaatccgt ggcgctgtcg cagagcggta aatagctcgt gggccgcgat
     3841 ttcagctccg ccgcgtgaaa actccggatg accatgcgat gcaatcagca cccgcacggc
     3901 ctgtttggct tgcgcgaacg aaggcacctt gagtcgttgc gtgggcatca gggagcgacc
     3961 gccagggtcc gggccgaggc cgaaaaccag cgtcgctgat gaacccaggc gttgtacaac
     4021 gtcaggttga tggagcgtgg gtcggtcgag gcactgtgtc gcggagactg caactgatag
     4081 agctccacct catcatccac ggcacacgtc agctcgcgtg cacgtacctt gagacacaga
     4141 tctgaatcct cgaattcgcc cgccagatag gactcgtcga aaccaccgag tttcagcgcc
     4201 agcgaacgcc gcatcagcat gcagccgtgg gctacggcat cgcatcggtg cagaccgatc
     4261 ttgggttcag gacgacgccc ttcattcaga tggtccggat actgccagtt gccaaattca
     4321 cgcatgggcc gatagcgata gccttcgtgc cggatcgaac catcctctga cagcaggcgc
     4381 gcgctcagca ggcccacgtt ggcatgtcgc tcgaggcggt caagcagtcg ctccagccac
     4441 tgttcattga caggaagtac ctcgggtcgc atgaagcaaa cgtactggcc gcgtgccgcg
     4501 cgcaagccgg cgttgaatgc cggacctgga cccactggag acggcagcag cacgaggcga
     4561 aagggaatgc caaagcgctc ataggcggat tgggccagcg tctgagcgtt tcggcgccag
     4621 cgcacatcgt ccaggacgta catcaggtcg atctgcttcg gattccagcg ccggctgaaa
     4681 agtgccaact gg
//
LOCUS       DATMRM010000135        10934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3165561, whole genome shotgun sequence.
ACCESSION   DATMRM010000135 DATMRM010000000
VERSION     DATMRM010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10934
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3165561"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VNZ06_03845"
     CDS             <1..302
                     /locus_tag="VNZ06_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19915.1"
                     /translation="EALSDREDARLARRLAQDYVARALGRDNTRATQVAQRGLTIDPG
                     FRPRSGAQCLRVADLLRLAGDRQSAQSLLRDFATHFPGDPAIADAQDLLARLTRQ"
     gene            complement(313..1770)
                     /locus_tag="VNZ06_03850"
     CDS             complement(313..1770)
                     /locus_tag="VNZ06_03850"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HWW19916.1"
                     /translation="MNTTKLRTSAMAVGAVSLLALGYGFGSSAIGRTAPSTSTASSET
                     SAPRNTVALPDFSSIVAQYGPAVVNVRVSGMMRTGAHQWHSPNFNFDENDPFSQFFRQ
                     FQQQMPQETPMHGIGSGFIVSSDGIILTNAHVVDGAEQVSVKLTDKREFQARVLGKDK
                     ATDIAVLKIDARNLPVVKIGDAGRERVGEWAVAIGAPFGFENTATAGIISAKARSLPN
                     QGYVQFIQTDVPVNPGNSGGPLFNLNGEVVGINSQIYSGSGGYQGVSFAIPINLAMQV
                     EQQLVKNGKVERGQLGVSIQSVDQSLAQSFGLKEAEGALISSVTPGGPGDKAGLQSGD
                     VILKFNGQDVADAGQLPSMVGGVAPGDTVHLTVWRKGSQRDVQVKLGDANAGQTLATS
                     AGEEGKARLGLAVRPLSSDERSQADVSGGLVVESVSGPAAKAGIEPGDIVLALNGQPV
                     TSVSQLREQAAKAGHQIALLIQHDDARVFVPLDLG"
     gene            complement(1924..3237)
                     /locus_tag="VNZ06_03855"
     CDS             complement(1924..3237)
                     /locus_tag="VNZ06_03855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW19917.1"
                     /translation="MNSIRRNLLLALLGALLLTLLLGAAATYRAAVQQAEVLLDYQLR
                     QTALALRDQAFGAITEPALPADAGDLDYSVQVWSEDGVSIYYSYPPHVFPNRAQLGFS
                     TIRTSEGPWRVFAMQGRREVIQVAQPISRRDQLAAHAALQSMRPYLLLLPLMGLLIWF
                     LVGRGLRPIAHLAHALRTRTPRSLEPVSESVPEEVQPLVRALNDLLARLGAALLAQRQ
                     LVADAAHELRTPLAALLLQTQLVERAGSDAQRATALAELKRGIERATRVMQQLLTLAR
                     EEPAAVRTAFVPLQLQTLLESVAAAFHVLAEAKAIAFSVNTAGCAARVTGDEASLQTL
                     FANLLDNALRYTPAGGSVSLSLREQQGRPLVSVEDSGPGIAPEDRARVFDRFYRRPGV
                     EEPGTGLGLAIVRAIAERHHLAIHLGTSAAGGLSVTVEFPGSSAS"
     gene            complement(3234..3911)
                     /locus_tag="VNZ06_03860"
     CDS             complement(3234..3911)
                     /locus_tag="VNZ06_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW19918.1"
                     /translation="MPGALSVRLLLVEDDAMIGRSLQQGLKQDGYAVDWVRDGHGARL
                     SLQEDGYALVLLDLSLPRLSGRELLSELRQRHNPIPVLIITAHDSIADRVAGLDAGAD
                     DYLVKPFSFEELSARIRALLRRHGGVAEPLLRQGSLTLNPSTHEVRLGEALVHLSARE
                     FALLEAMMRRPGMPLSRSQLENSIYGWDQEVDSNAVEVHIHALRRKLGAGCIENVRGV
                     GYRVPKQ"
     gene            complement(3912..4613)
                     /locus_tag="VNZ06_03865"
     CDS             complement(3912..4613)
                     /locus_tag="VNZ06_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWW19919.1"
                     /translation="MLEVRRSGERGYADHGWLKSFHTFSFADYFDPQHVEFGPLRVIN
                     EDRVAPGAGFGTHGHRDMEILSYVLAGGLAHRDSMGNGSTIRPGDVQRMSAGRGVQHS
                     EFNASQSEPLHFLQIWIQPDRTGIAPGYEEKHFSTQERRGQLRLIASADRAGGSLLIH
                     QDARVYAGLIDGPESARLPLSPGRRVYVHLARGTLKANDTALSAGDALKLTDTGTLLL
                     NDGHDAEVLVFDLPV"
     gene            4679..6001
                     /locus_tag="VNZ06_03870"
     CDS             4679..6001
                     /locus_tag="VNZ06_03870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWW19920.1"
                     /translation="MSVEQCDIAIIGGGPAGSTAATLLAKRGYRVIALEKEHHPRFHI
                     GESLLPMNLPVFERLGVLEKVRAIGLYKPGADFEADNERGYNNYAFSRAIGNSPPHAY
                     QVWRQEFDQMLYQHARECGADAREGQEVLAFNQLSARETLLSVRSDDGRDYQIQARYL
                     IDASGRDSFVSARKKMRRKNTEHQSAAIFGHFRRAAMRPGEDAGNISIYRFEHGWMWM
                     IPLRSGVMSVGAVCRPDYLKQRKGRTVEFLIETLKLNPALWARLREAELIGNEIRVTG
                     NYSYDSSRMGGIGWVLIGDAFAFLDPVFSSGVYLAMSGAEQAVQVVDTALREPRREAA
                     LLRQLEKRQRAGMRRFSFFIYRFNGPVMTQLFRSPRNIWKLEQAVISMLAGDLFDTPE
                     VLRRLRLFKLIYALGAMLDFRRWLRETRYRLGQARSQFTGGTTPLDKT"
     gene            6076..7707
                     /locus_tag="VNZ06_03875"
     CDS             6076..7707
                     /locus_tag="VNZ06_03875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HWW19921.1"
                     /translation="MSTVITAPGFFTRERTVAQPGFSRWMVPPAALAIHLCIGMAYGF
                     SVFWLPLSKSIGISKAVACPKEMDFFTSLTQTSCDWKVADLATTFTIFIVVLGVSAAI
                     WGGWLERAGPRKAGLVAAVCWCGGFFIGAAGIVTHQLWMLWVGPGLIGGIGLGLGYIS
                     PVSTLIKWFPDRRGMATGMAIMGFGGGAMIGSPLADRLMKLFATPSSVGVWQTFVTLG
                     AIYFVFMVGGALSYRVPPEGWAPRGWQPKPSANKSLITDRDVSLSEASRTPQFWLLWG
                     VLCLNVTAGIGILAMASPILQEVFAGQLIHVKAGFDELTSSQKAAIATIAAGFTSLLS
                     MFNIGGRIFWASLSDYLGRKATYMVFFVLGFLLYASIPWTANGGQRALFVAFCCVILT
                     MYGGGFATIPAYLSDLFGTRMVGAIHGRLLTAWSAAGIIGPQLVARVRQYQIDHGVKG
                     AEIYNVTMYILAGLLVGGFICNLLVRHVHERHYMKAPEAAPTPAAAVHSGASAAVMDS
                     AEGVGALVVAAWLAVWIPIAWGVWVTLQKAVLLFK"
     gene            complement(7726..9039)
                     /locus_tag="VNZ06_03880"
     CDS             complement(7726..9039)
                     /locus_tag="VNZ06_03880"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWW19922.1"
                     /translation="MNATIQELVPENRVSDKQPSRHEELLDQSARQLNVRGVLRTSLA
                     EIAAKLGVSRNALYYYVSGREELLFQCYQRAAQISAARLTDAIRAGGNAADVLTQFVV
                     RTMDPKGPEIAYRTEIAMLNPAQRVEIQALREALIMRLAELIETGQRQGVIRACDPDI
                     NARVALSVTSWVPLSRRWMAAVEPQGASRLQMAAVQTLVEGLSPHVSPGYFQPLDLSE
                     LVRRGHRAFDRDGASEAKRELVLRTASRLFNRKGIDSTSLEEIAAQVGATKRTVYHHL
                     GNKQALVMACYERAYRIFFFIIDGMKAHPGTRLEALAAALHAVALAYPDEDLTPLSPL
                     VGFATLVPKARAKMNDYGLRLSSDYRSVLRQGIKEGSLREVDVTPHTLMLAGLTSWLV
                     REDMPTEPTRRQQIAQEIANLVMVGLATRAVHADSARAGPQSCAP"
     gene            9342..9563
                     /locus_tag="VNZ06_03885"
     CDS             9342..9563
                     /locus_tag="VNZ06_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19923.1"
                     /translation="MKPSAVHHGTVELRKPKFTLKDNGIEVQEEELCAVKAQVLYKMR
                     CECGRSWFEIELPRLVKCPACDKLGLVST"
     gene            9789..10730
                     /locus_tag="VNZ06_03890"
     CDS             9789..10730
                     /locus_tag="VNZ06_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWW19924.1"
                     /translation="MSNDTLLQSRRNALKCLAYGSAGTLFTLAGGVLSPIDLAFAQER
                     NTSMAPAGTPLFVQISDTHIGFNKEANPDVNGTLSQTIQIVNAMPKQPALILHTGDIT
                     HLSKPEEFDTAQQLMSKLRVSELHTTPGEHDTTDPTVSEYFNRFGKASGNKGYYSFDH
                     AGVHFIALINVLQFKAGGLGTLGADQIAWVTQDLKGRSASTPIVVFAHMPLWTIYEPW
                     GWGTGDADQLMAQLQRFGSVTVLNGHIHQIVSKVEGNMTFHTARSTSYPQPTAGQGSG
                     PGPLKVASEQLPKMLGVTSVTEVKHPRSLALTDSTLA"
BASE COUNT         1988 a         3559 c         3425 g         1962 t
ORIGIN      
        1 ttgaggccct gagcgatcga gaggatgcac gcctggcgcg ccggctggca caggactacg
       61 ttgcccgcgc gctgggacgc gacaatacgc gcgccacgca ggtcgcgcag cgcggattga
      121 caatcgatcc tggtttccgg ccacgctccg gcgcgcagtg tctgcgcgtg gcggatctgc
      181 tgcgactggc cggagaccgg caaagcgcgc agagcctgct gcgcgacttt gccactcatt
      241 ttcccggtga tccggccatc gccgatgcgc aggacctgct ggcccgactc acccgacaat
      301 aggcaccgcc cgttagccga gatcgagcgg gacgaatacc cgcgcatcgt cgtgctgaat
      361 cagcagtgca atctggtgtc cggccttcgc agcctgctcg cgtagctgcg acaccgaggt
      421 gaccggctgg ccgttgaggg ccagcacgat gtcacccggc tcaatgcccg ctttcgcggc
      481 cgggccgctg acactttcca cgaccagtcc gcccgacaca tccgcctgtg agcgctcatc
      541 ggagctcagt ggacgcactg ccaggcccag tcgggccttg ccctcctcgc ctgcggacgt
      601 ggcgagcgtc tgcccggcat ttgcatcgcc gagcttcacc tgcacgtcgc gttgtgagcc
      661 tttacgccat accgtcagat gcacggtgtc gccgggcgcc acgccgccca ccatcgaggg
      721 cagctgcccg gcatcggcga cgtcctgacc gttgaacttc aggatcacgt caccggactg
      781 cagaccggct ttgtcgccgg gaccacccgg tgtcaccgag ctgatcagcg caccctcggc
      841 ctccttgagt ccgaaggatt gcgcaagcga ctgatccact gactgaatcg acaccccgag
      901 ctgtccgcgc tcgaccttgc cgttcttcac cagttgctgc tcgacctgca tcgccaggtt
      961 gatcgggatg gcgaatgaca cgccctgata accaccgctg ccgctgtaga tctgcgagtt
     1021 gatgccaacg acctcgccgt tcagattgaa cagcggcccg cccgagttac cgggattgac
     1081 cggtacatcg gtctgaatga actgcacgta cccctggttg gggagcgagc gcgccttggc
     1141 cgaaatgatg cccgcggtcg cggtattctc gaacccgaac ggtgcaccaa tcgcgaccgc
     1201 ccattcaccc acccgctccc tgccggcatc gccgatcttc acgaccggca ggttgcgggc
     1261 atcgattttc aggaccgcga tatcggtcgc cttgtccttg cccagcaccc tggcctgaaa
     1321 ttcacgcttg tcagtgagct tcacgctcac ctgctcggcg ccgtccacca cgtgcgcatt
     1381 ggtgaggatg atgccgtcgg aactgacgat gaatccggaa ccgattccat gcatcggtgt
     1441 ctcctgcggc atctgctgct gaaactgccg gaagaactga ctgaacggat cattctcatc
     1501 aaagttgaag ttcggcgagt gccactggtg cgcaccggta cgcatcatgc ccgacacgcg
     1561 cacgttgacc accgcggggc cgtactgcgc gacgatcgaa ctgaaatcag gcagcgcgac
     1621 cgtgttgcgc ggcgccgagg tttcgctgct cgccgttgac gtcgatggcg cggttcgtcc
     1681 gatggccgaa ctgccgaagc catatcccag tgccagcagc gatacggcgc ccaccgccat
     1741 cgcgctggtc ctcaatttcg tggtgttcat cgccaactcc tgaagctcgt tcgtcacaag
     1801 gcgcggccgg tcggccgctg tataacgaca cgctatccgc aaaaacttaa gagtgactta
     1861 aggacccgca gagactcgag aagcgctcga gagcggccaa aaaggccctt gagacagagt
     1921 gactcaactg gcagaactgc cagggaattc aacggttacc gacaggccac cggcggccga
     1981 ggtgccgagg tgaatggcca gatgatgccg ttcggcaatg gcgcgcacga tcgccagccc
     2041 caagccggtg ccgggctcct cgacgccagg acgacgatag aaacgatcga acacgcgcgc
     2101 gcgatcctcc ggcgcaatgc cgggaccgga atcctcgacg ctgacgagcg ggcgtccctg
     2161 ctgctcgcgc aagctcaacg agacgctgcc gccggcgggc gtgtagcgca gcgcgttgtc
     2221 gagcaggttg gcaaacagtg tctgcagcga ggcctcatcg ccggtcacgc gcgccgcgca
     2281 tccggcggtg ttgaccgaga aggcgatggc cttcgcctca gccagcacat ggaatgccgc
     2341 tgcgaccgac tcgagcagtg tctgcagctg cagcggcacg aacgccgtgc gtacggcagc
     2401 gggttcctcg cgcgcgaggg tcagcagctg ctgcatcacg cgcgtcgcgc gctcgatgcc
     2461 gcgcttgagc tcggccagag ccgtggcgcg ctgcgcatca ctgcccgcac gctcgaccag
     2521 ctgtgtctgc agcagcagcg ccgcgagcgg cgtgcgcagc tcatgcgccg catcggccac
     2581 gagctgtcgc tgtgcgagca gcgcggcacc cagtctggcc agcaggtcgt tcagcgcccg
     2641 caccagtggc tgcacttcct cgggcaccga ttcggacacc ggctcgagcg agcgtggcgt
     2701 acgggtcctc agcgcgtgcg ccagatgcgc gatcggcctg aggccgcggc cgaccaggaa
     2761 ccagatcagc aggcccatca gcggcaacag cagcagatag ggacgcatgc tctgcagcgc
     2821 cgcgtgtgcc gcgagctgat cgcgacgcga gatcggttgc gcgacctgaa tcacctcacg
     2881 ccgcccctgc atcgcgaaca cccgccaggg accctcactg gtccggatgg tggagaagcc
     2941 cagttgcgcg cggttcggga acacgtgcgg cggatacgag tagtaaatac tgacgccgtc
     3001 ctcgctccac acctgcaccg aatagtcgag atcgcccgcg tccgccggca gcgcgggctc
     3061 ggtgatcgca ccaaaggcct gatcgcgcag cgccagcgcc gtctgacgca gctgatagtc
     3121 gagcagcacc tccgcctgct gaaccgccgc gcgataggtg gccgccgcgc ccagcagcag
     3181 cgtgagcagc agcgccccga gcagcgccag caacagattg cggcgaatgg aattcattgc
     3241 ttcggcacgc ggtagccgac cccgcgcacg ttctcgatgc agcccgcacc gagcttgcgg
     3301 cgcagcgcat ggatgtgcac ctcgaccgcg ttgctgtcca cctcctgatc ccagccgtag
     3361 atcgagttct cgagctgcga gcgcgacaag ggcatcccgg gtcgtcgcat catcgcctcg
     3421 agcagcgcga attcacgcgc tgacagatgc accagcgcct cgcccagccg cacttcgtgc
     3481 gtcgacgggt tgagtgtcag gcttccctgc cgcagcagcg gctcggccac gccgccgtgg
     3541 cgccgcagca gcgcacgaat gcgcgcgctg agttcctcga agctgaaagg cttgacgagg
     3601 tagtcatcgg cgccggcatc cagccccgcg acgcgatcgg caatcgagtc gtgcgcagtg
     3661 atgatcagca ccggaatcgg attgtgccgc tgccgcagtt cactgagcag ttcgcgcccg
     3721 ctcaggcgcg gcagcgacag atccagcaac accagcgcgt agccgtcctc ctgcagggac
     3781 agccgcgcgc cgtgaccgtc gcgcacccag tccaccgcgt aaccgtcctg cttcagcccc
     3841 tgttgcaggc tgcggccgat catcgcatcg tcttccacca gcagcaggcg cacgctcagc
     3901 gcccccggca ctcagacggg cagatcgaac accagtactt cggcgtcgtg accatcattc
     3961 agtaggagtg tgccggtgtc ggtgagtttc agggcatcac cggccgacaa ggcggtatcg
     4021 ttggctttga gcgtaccgcg tgccagatgc acgtacaccc ggcggcccgg cgagagcggc
     4081 agtcgtgccg actcgggccc atcgatgagg cccgcgtaca cgcgggcatc ctgatggatc
     4141 agcagcgagc cgccggcccg atccgcagag gcgatcagcc gcaactggcc gcgccgctcc
     4201 tgcgtgctga agtgcttctc ctcgtagccc ggcgcaatgc ccgtgcggtc aggctggatc
     4261 cagatctgca gaaaatgcag cggctcgctc tgcgaggcgt tgaactcgct gtgctgcaca
     4321 ccccggccgg cgctcatgcg ctgcacgtcg cccggccgga tggtcgagcc gttgcccatc
     4381 gaatcgcggt gggccagccc ccccgcgagc acgtagctca ggatttccat atcccgatgg
     4441 ccgtgggtgc cgaaacctgc gcccggggcc acgcggtcct cgttgatgac gcgcagcggt
     4501 ccgaattcga cgtgctgtgg atcgaagtag tcggcaaatg aaaaagtatg aaaggacttc
     4561 agccagccgt gatcggcgta gccgcgttcc ccactgcgtc ggacttcaag cataacagtg
     4621 acctccgtgg ggatcgacaa tgggggcgaa ttgctcaaaa taaagcataa ttgccggcat
     4681 gagcgtcgag caatgcgata ttgccatcat cggtggcgga ccggccggca gcaccgcggc
     4741 gacactactg gcgaagcgcg gctaccgggt gatcgcgctg gaaaaggagc accacccccg
     4801 atttcacatc ggtgagtcgc tgctgccgat gaacctgccg gtgttcgagc ggctgggggt
     4861 gctggaaaag gtacgcgcaa tcggcctcta caagccgggg gccgatttcg aggccgacaa
     4921 cgagcgcggc tacaacaact acgccttcag ccgggcgatc ggcaacagcc cgccgcacgc
     4981 ctatcaggtc tggcggcagg aattcgatca gatgctctac cagcacgcgc gcgagtgcgg
     5041 cgcggacgcg cgtgagggtc aggaggtcct ggccttcaat cagctcagtg cgcgcgagac
     5101 gctgctgagc gtgcgctccg atgacggccg cgactatcag atccaggcgc gctatctgat
     5161 cgacgccagc gggcgcgaca gctttgtgtc cgcgcgcaag aaaatgcgcc gcaagaacac
     5221 cgagcatcag agcgcggcga tcttcggcca cttccgccgc gccgcgatgc gaccgggcga
     5281 ggatgccggc aatatcagca tctatcgctt cgagcacggc tggatgtgga tgattccgct
     5341 gcgcagcggt gtcatgagcg tcggtgcggt atgccggccg gactacctca agcagcgcaa
     5401 gggacgcacg gtcgaattcc tgatcgagac gctcaagctc aatccggcgc tgtgggcacg
     5461 cctgcgcgag gctgaactca tcggaaatga aatccgcgtg accggcaatt actcctacga
     5521 ctccagtcgc atgggcggca ttggctgggt gttgatcggc gatgccttcg cattcctcga
     5581 tccggtgttc tcttcagggg tgtacctggc gatgagcggt gccgagcagg cggtgcaagt
     5641 ggtggacacg gcgctgcgcg aaccgcggcg cgaagccgcg ctgctgcggc aactcgagaa
     5701 gcgacagcgc gcgggcatgc gacgtttctc cttctttatc taccgtttca atgggccggt
     5761 gatgacgcag ctgtttcgca gcccgcgcaa catctggaag ctcgagcagg ctgtgatctc
     5821 gatgctggcc ggggacctgt tcgatacgcc cgaggtgctg cgccggctgc gactgttcaa
     5881 gctgatctat gcattgggag caatgcttga tttcagacgc tggctgcggg agacacggta
     5941 tcggctgggc caggcgcgat cacaatttac ggggggaacc acaccgctcg acaagactta
     6001 atgcggtgcg tctatcactg catctgacca tgccttagac tcgtaatcat aagaatcaga
     6061 ctgggggatc catcagtgtc tacggtcatc acagcgcccg gatttttcac gcgcgaacgc
     6121 accgtcgctc aaccaggatt cagtcgctgg atggtaccgc cggccgcact ggccattcat
     6181 ctgtgtatcg gcatggccta tggtttttcg gtgttctggc tgccgctgtc gaagtcgatc
     6241 gggatcagca aggcggtagc ctgtccgaag gagatggatt tcttcacgag cctcacgcaa
     6301 accagctgtg actggaaggt ggcggatctg gccaccacgt tcacgatctt catcgtcgtg
     6361 ctcggtgtct ccgctgccat ctggggcggc tggctcgagc gcgccggtcc ccgcaaggcc
     6421 gggctggtcg cggccgtgtg ctggtgcggc ggatttttca tcggtgccgc gggcatcgtc
     6481 acgcatcagc tgtggatgct gtgggtggga cccggcctga tcggtggcat cggcttgggt
     6541 ttaggttaca tctcaccggt ttcgacactg atcaaatggt tcccggatcg ccgcggaatg
     6601 gcgaccggca tggcgatcat gggattcggt ggcggcgcga tgattggctc accgctggcc
     6661 gatcgcctga tgaaactgtt cgccacgccg agctcggttg gcgtgtggca gaccttcgtt
     6721 accctggggg ccatctactt cgtgttcatg gtcggtggcg cgctgagcta tcgcgtgccg
     6781 cccgagggct gggcgccgag gggctggcag cccaagccga gcgcaaacaa gagtctgatc
     6841 accgaccgcg acgtgtctct gagcgaagcc tcgcgcacac cgcagttctg gctgctgtgg
     6901 ggcgtgctgt gtctgaacgt caccgcgggc atcgggattc tggcgatggc ctcgccaatt
     6961 ctgcaggaag tcttcgccgg tcagttgatc cacgtcaagg ccgggttcga tgagctgacc
     7021 agctcgcaga aggccgcgat cgcgaccatc gcagcgggat ttaccagcct gctgtcgatg
     7081 ttcaacattg gcggacgcat cttctgggcc tcgctatcgg actatctcgg ccgcaaggcg
     7141 acctacatgg tcttcttcgt gctgggcttc ctcctctacg catcgatccc ctggaccgcg
     7201 aacggcggac agcgggcact gttcgtcgcc ttctgctgcg tcatcctgac gatgtacggc
     7261 ggcggcttcg cgaccattcc agcgtatttg tcggatctgt ttgggacgcg tatggtcggc
     7321 gcgattcacg gccggctgct gacggcgtgg tctgctgccg gcatcattgg cccacaactg
     7381 gtcgcccgcg tgcgtcagta ccagatcgat cacggcgtca aaggcgccga gatctacaac
     7441 gtcacgatgt acatcctggc cgggctgctg gtcggtggct tcatctgcaa tctgttggtg
     7501 cgccacgtac acgagcgcca ctacatgaaa gcgcccgaag ccgcaccaac gccggccgct
     7561 gcggtgcatt caggcgcctc ggcggcggtg atggatagcg ccgaaggggt cggcgcactg
     7621 gtcgtggcgg cctggctcgc cgtgtggatc ccgatcgcct ggggcgtgtg ggtcacgctg
     7681 cagaaagccg tactgctttt caaataaacc ttcagctaga gatcgctacg gtgcgcacga
     7741 ctgcgggcct gcccgcgctg agtccgcatg gacggcccgc gtggccagac ccaccatgac
     7801 gagattggcg atttcctggg cgatctgctg acgtcgagtc ggctcggtcg gcatgtcctc
     7861 gcgtaccaac cagctcgtga gtccggccag catgagggtg tgcggagtca cgtccacctc
     7921 ccgcagcgaa ccttctttga tgccctggcg cagcaccgag cggtagtccg acgacaagcg
     7981 caggccgtag tcgttcatct tggcgcgcgc tttcggcacc agggttgcaa agccgaccag
     8041 cggtgacagc ggcgtgagat cctcatccgg ataggccagt gcgaccgcgt gaagcgccgc
     8101 ggcgagcgct tccaggcgcg tgccgggatg cgccttcatg ccgtcgataa tgaagaagaa
     8161 gatgcggtag gcgcgctcgt agcaggccat gaccagtgcc tgcttgttgc ccaggtggtg
     8221 gtacaccgtg cgcttggtcg caccgacctg agcggctatc tcctccaatg aggtcgagtc
     8281 gatgcccttg cgattgaaca gacgcgacgc cgtccgcagc accagctcgc gcttggcctc
     8341 gctggcgccg tcgcgatcga acgcgcggtg accgcggcga accagttcgc tcaagtccag
     8401 cggctgaaag tagccgggcg agacatgggg gctcagtcct tcgaccaggg tttgaaccgc
     8461 ggccatctgc aggcgggacg cgccctgcgg ttcaaccgcc gccatccagc ggcggctcag
     8521 tggcacccaa ctggtgacgc tcagcgcgac ccgggcattg atgtccgggt cgcaggcccg
     8581 gatgacaccc tggcgctgac cggtctcgat cagctcggcc agccgcatga tcaacgcctc
     8641 ccgcaatgcc tggatctcca cgcgctgcgc cggattgagc atcgcgatct cggtgcggta
     8701 cgcaatctcc ggacccttcg gatccatcgt gcgaaccacg aactgcgtca gtacgtcggc
     8761 cgcgttgcca ccggcgcgaa tcgcgtccgt gaggcgcgcc gccgagatct gcgcggcgcg
     8821 ctgatagcac tggaacagca gctcttcgcg cccggaaacg tagtagtaca gcgcgttacg
     8881 cgagaccccg agcttggcgg cgatctccgc cagcgacgtg cgcagcactc cgcgcacgtt
     8941 caactgacgt gcggactggt cgagcaactc ttcgtggcgg cttggctgct tgtcggaaac
     9001 cctgttttcc gggacgagtt cctggatcgt tgcattcacg atcacatcat cctaccgcag
     9061 tccccgcgag cagcatgtta ctgaatattt atcacagcgc cgggtcgctt tactcttcag
     9121 tgtccttcat cacctgcacg tcaaggcact cccggccgcg caggagccgc cagcgcgcag
     9181 tgtcgtcgtg aagattccca caggcgcagc cgcaccgttt tcgctcattt ttcgcgccca
     9241 ccgcaggcca gcagatcaat tagttacgcc ctgtttgcat cctacaatct aatgtgcgat
     9301 gtttgacata agaggtagcg aaaacaacgg agatgcctga catgaagccc tcggctgtgc
     9361 accacggtac cgtcgaactg cgtaagccga aattcacgct gaaggacaac ggcatcgaag
     9421 tgcaggaaga ggaactgtgc gcggtgaagg cgcaggttct ctataagatg cgctgcgagt
     9481 gcggacgctc ctggttcgaa atcgaattgc cccgtctggt gaaatgcccc gcgtgcgaca
     9541 agctcggact cgtcagcacc tgaggatcgc gtcccatata tattgaggcc catgcagccc
     9601 cgacaccggc gggctcaatg ctttgtcata tttgatagct agccggccac tgtctgctcg
     9661 caggatttga caggtcgggc gcgcagcgcg tcaaatcctt ttgcctaaca gccgtttccg
     9721 ttgtttacgg gaataaatgc accgctgatg tggtcccgtt cgatgaccac atcattagga
     9781 gacggcccgt gtcgaacgat accctcttgc agtcgcgccg taacgcgctc aagtgtctgg
     9841 cttacggaag cgccggcact ttgttcaccc tcgcgggcgg cgtgctctcg ccgatcgatc
     9901 tggcgttcgc tcaggagcgc aacacgagca tggcgccggc aggcacgccg ctgttcgtgc
     9961 agatcagcga tacccacatt ggcttcaaca aggaggccaa tcctgacgtc aacggcacgc
    10021 tgtcgcagac gatccagatc gtcaatgcga tgccgaaaca gccagcgttg attctccaca
    10081 ccggtgacat cacccacctg tcgaagcccg aggaattcga taccgcccag cagctgatgt
    10141 cgaagctgcg ggtcagcgag ctgcacacca cgcccggcga gcacgacacc accgatccta
    10201 ccgtcagcga gtatttcaat cgcttcggca aggcgtcggg caacaagggc tactacagtt
    10261 tcgatcacgc gggcgtgcat ttcatcgcgc tgatcaacgt gctgcagttc aaggccggcg
    10321 gcctgggcac tctgggcgcc gatcagatcg cctgggtgac gcaggatctg aaaggccgct
    10381 ccgccagcac gccgatcgtc gtgttcgcgc acatgccgct gtggaccatc tatgagccgt
    10441 ggggctgggg cacgggcgat gccgatcagc tgatggcgca gctccagcgc ttcggttcgg
    10501 tgaccgtgct gaatggacac attcaccaga tcgtctccaa ggtcgaaggc aacatgacat
    10561 tccacacggc gcgctccacg tcctatccgc agccgactgc cggacagggc tcgggccccg
    10621 gcccgctgaa agtggcctcc gaacagctgc cgaaaatgct gggcgtgacc agcgttaccg
    10681 aagtgaagca cccgcgctca ctcgcactca cggatagcac cctggcttga ggccacccac
    10741 cccacggata aggaaagtca catgaccccc acccatcgaa aactgctggc actgaccagc
    10801 accgcagcac tgtggctggc gatgaccggt tcagcgccgc tcgtcgctgg cgatgatccg
    10861 aacacgatcg tcgtgaagag cttcatgttc acaccgatga acctgaaggt gaaggccggc
    10921 accaagatca cctg
//
LOCUS       DATMRM010000136        24307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_389254, whole genome shotgun sequence.
ACCESSION   DATMRM010000136 DATMRM010000000
VERSION     DATMRM010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 24307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24307
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389254"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VNZ06_03895"
     CDS             complement(<1..336)
                     /locus_tag="VNZ06_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19925.1"
                     /translation="MSKPPGTTTDFTASDVLSAQFWTGRAERTGPGIDVGCTKIAVDL
                     QNMTRQSADDCIRRSLETFRDSTSADAAFTIFLDSGGQHIESVLCARGHFAQCHPEAL
                     RGVAIDSLPW"
     gene            complement(408..1166)
                     /locus_tag="VNZ06_03900"
     CDS             complement(408..1166)
                     /locus_tag="VNZ06_03900"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF374 domain-containing protein"
                     /protein_id="HWW19926.1"
                     /translation="MNDEPSDGTLRNTIYRRVTVSGRRMTPTRRLLYRLAAPILLAVI
                     RTWWRSCRIVAISGQEHLDAVLARWPSFLPCYWHQHQLFGARFLLMLQQQGRLRAGFL
                     ISPSVDGELGAMMVRKLGGYVIRGSSSHTGALALKDYFQALIKQQVSPIITPDGPRGP
                     KFVFKPGAILLSQLSQRPMLPIAYAAARAKLVAWDRFVIPLPFTRIAIGIGPSREVPK
                     MATPQQIEALQLEMAQALKASFEAARAALAANSV"
     gene            1275..3266
                     /locus_tag="VNZ06_03905"
     CDS             1275..3266
                     /locus_tag="VNZ06_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWW19927.1"
                     /translation="MSSSPLYTATTLTALIESLRENSSGGLHYHDSERETRRVSFAEL
                     YQRALGILYHLQRLGARPGDKLILLLSGNEPFIDAFWAAMLGGIVPVPIAPGISDEHR
                     HKLLRIARKLHDPFIYTEQRLLERVAVLAAASGEAARTTFEALRARAFLVDQLHDISR
                     AGKLHRALASDTAFIQFSSGSTSEPKGVVLTHANLLANIRAIGFAAGLNASTITLSWM
                     PLTHDMGLIGFHLVLLGHGANAHLMPTELFVRRPLLWLALASRVRATMLCSPNFGYRH
                     YLKMLGERRIEDLDLSSVQLIYNGAEPISVDLMEEFLSRTAPARLARNAMFPVYGLAE
                     ASLAVSFPQPGAPSQVLSLNRHQLNVGEKPQTDGVAERDVLRLVGVGRAVPYCELRIT
                     DDRDLPLGERQVGHIQIRGDNVTGGYFEDPELNRSSFSADGWLRTGDLGLIHRTQLYI
                     TGRHKEILFVNGQNYYPHDLEGLLEQVEGLELGKVVVAGVRPPGAQEDELLVFVLHRG
                     TLADFMPLAREVARRIAESAGLEVSAVIPVKRIPKTTSGKIQRHALETEYLTGAFAAE
                     LAQIGTLRATQAKPAVSGSDIEQALRDICEQELEGRSIDRDESLFDIGASSIKLLAIH
                     QRLEQQWPGRVDVTDIFDHPSIAALARFIEQSLTVREQV"
     gene            3263..4024
                     /locus_tag="VNZ06_03910"
     CDS             3263..4024
                     /locus_tag="VNZ06_03910"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWW19928.1"
                     /translation="MSDRLQRAAQYLAALRQAGPRPVSLPQELAPQNAAQAYELQQRV
                     ARELKVSAGGWKVAMSGLNAGSYAPVFATDLHASGARVTSAISQQLGVEPEVAFTLKR
                     TLPAGRRYTREQVIEAIGSAHAAIEIVISRFQRHETAAVLDRLADNISNGGLVLGPAL
                     DRWQHLDFSALPLTLTLTAANGEARVHQSRGGHPQADPLLPMTWIVNERAALGFGMRQ
                     GEVVTTGSFAGLHYAARGTRVRVEFEGLGGAELYG"
     gene            4111..5160
                     /locus_tag="VNZ06_03915"
     CDS             4111..5160
                     /locus_tag="VNZ06_03915"
                     /inference="COORDINATES: protein motif:HMM:NF044415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19929.1"
                     /translation="MGSAAALQVEYPRLDEIRQRPAAWWQDVLALIHFNDGECSQPQL
                     PAAVNIPVAQVRVPMLAGADGAVEVWRLSGPMRSGQSGRVAYRHNGRLLFASLSISEQ
                     EFPGGSDADSATALRCATEAGYSELFSTIEALHFPHPLRIWNFLPAINGQSEGGERYW
                     HFNGARQDVFLGFNRCITGNVPAASAVGAVSTSPLTIYCIASASAPISLENPRQRAAW
                     AYPPQYGPKSPTFARACIEGDNAHTLFISGTSSIIGHESAHAGDECEQTRETLRNIRA
                     LIGAANERIGAQRFSLPELHYKVYVRHPAHLSNIERELRREIGERPAVMYVQADICRR
                     ELMVEIEAVGTMVSG"
     gene            5157..5996
                     /locus_tag="VNZ06_03920"
     CDS             5157..5996
                     /locus_tag="VNZ06_03920"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HWW19930.1"
                     /translation="MSGQRAAAAALGLLGALTCGSACADPQPLWDFGLGVGAVVFNDY
                     RGASAMHGYPVPVPYFIYRGAFLRADRDGVHGRFLDSLHLEFDISADATPPVFSHHSA
                     PRQGMPNLASTVELGPSLTWHAWRSQDQRLRFDVRSPVRNAITIDSPPRSIGWIYAPN
                     LSVDYLGKGRARGWNFGLLTGPLYSQRQYNRYYYAVAPQYATASRPAYQPPGGYAGTQ
                     LLVSTSKRYRDYWFGAYLRHDWLQGAAFDPSPLVQQHSYWSGGFGFVWMIHSSSRMVD
                     SDE"
     gene            5989..6795
                     /locus_tag="VNZ06_03925"
     CDS             5989..6795
                     /locus_tag="VNZ06_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWW19931.1"
                     /translation="MNEVRAVGVTPPSKAAKTLLEYLILYSSLLLLALICLTWSLLAL
                     PLYLLLPRRAAIAFGRRGIMAGFRLYAYWTAAVGAYRLDLTAVDSLRGAPPMILAPNH
                     PSLVDAILILTRHPNLVCVMKSDLMENILYGPGARLARYINNSSPLRMVREAVAALED
                     GGTLLLFPEGTRSSKSPIGELKRSIAVIARQAQVPVQTLIIEIDQPFLSKARGLWSRA
                     SFPVTYRVRLGKRFDPPEDVETFMAELERYYRSELQGSLVADWLSRSPCP"
     gene            6786..7550
                     /locus_tag="VNZ06_03930"
     CDS             6786..7550
                     /locus_tag="VNZ06_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWW19932.1"
                     /translation="MPVATASTTHLVLIPSYNPGPKVFETVTAAREHWNPVWVVVDGS
                     IDGSAVALAAMAQHDPGLKVLTLPKNRGKGAAVAHGARRARLAGFTHILTMDSDGQHP
                     ADHIERFMQASVAEPQALVLGDPVFDASAPALRVGGRKVSNLFANLETLWLGIQDSLF
                     GFRVYPIDPLLKVMRRQPWMRRFDFDVEAAVRLVWRGLPPRNLKAPVRYFRPEEGGVS
                     HFHYGRDNALLSWMHLRLFIGFLLRLPVLLWRRVNV"
     gene            7730..7981
                     /locus_tag="VNZ06_03935"
     CDS             7730..7981
                     /locus_tag="VNZ06_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HWW19933.1"
                     /translation="MLEREIARLIVKGLNLPVQAEDIDPEAPLYGEGLGLDSIDILEI
                     ALLVSKQYGIQLRADNQENERIFRSLRHFAQYVAAQRTK"
     gene            7978..8625
                     /locus_tag="VNZ06_03940"
     CDS             7978..8625
                     /locus_tag="VNZ06_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19934.1"
                     /translation="MNYRWLRHGLVAASILTYALLQHYANTTPGAAALGAGLAITPLL
                     ITWLLLARRTPWPRIGVLLASLLAAALLAESWSRIEHSFPLMFMLQEAGVYLILAFGF
                     GRSLMSGQTPLCTHWATRIHGPLSEPVERYTRAVTLAWTVFFVAISLTSVLLYAFAAL
                     RVWSIFSNFLTLPLVALMFAIEYEVRRHRLPWMQRASLADTARAYFATTTRGRPI"
     gene            8640..10010
                     /locus_tag="VNZ06_03945"
     CDS             8640..10010
                     /locus_tag="VNZ06_03945"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWW19935.1"
                     /translation="MAMLPLLAHDRPDAIVAYRGGEPISAAQFLADTRTLARRLPAST
                     HILNACKDRYHFTVGLGASLLAGKCSVLPSTHTPEVIRHLRSFAPDVFCLSDDPQNSI
                     DLPQLLYTPDATKAQLRSGAGWNVPQIHAERLAAYIFTSGSTGVPLPHRKTWGRLVSC
                     VRAEVSRLTPLQRTPCAIVGTVPPQHMYGFESTVLLPLQSGSALSAERPFYPADIAEM
                     LERLRAPRVLFSTPVHLRALLASDIRLPKLELIVSATAMLSANIAREVEQRFGCELLE
                     IYGSTETGQIATRRTATEVRWRLWPDVRLQVSDSGAAAYGGHVGQPTPLADVVEITDP
                     QHFLLHGRTADLVNIAGKRNSVSYLNHQLNAIPGVLDGAFFVREDTDESIAGSGRLAA
                     VVVAPGLTASAITQALRERIDPVFLPRPLLLVEQLPRNETGKLPQHTLREIAAEAAVA
                     QPHLFV"
     gene            10007..10336
                     /locus_tag="VNZ06_03950"
     CDS             10007..10336
                     /locus_tag="VNZ06_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19936.1"
                     /translation="MTRQSLAVRIAADHPSLAGHFPGFPIVPGVVLLDEALHAIEQLQ
                     AAEPASWHITTVKFHHTLAPGEPLQLSVECHSDARVHFELRSARALVASGTIKRRARI
                     RAVVSGR"
     gene            10333..11265
                     /locus_tag="VNZ06_03955"
     CDS             10333..11265
                     /locus_tag="VNZ06_03955"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19937.1"
                     /translation="MNTSVTTRAAEWRRSPERGSRALLRAMMWLSLRLGRPAGRVILH
                     GIAAYFFLFAPKASQSMRQYLRRVLSRKPRASDRWKLILNFATMIHDRVYLLAERYHL
                     FDVSLQGEAMMKELVAAGGGAMLMGAHVGSFEILRWIGERQPNLRITITMYEENARKL
                     NEILAAIAPQNPPDVIAVGHLDAMLRIRERLDEGALVGMLADRTFGDEATLPVTFLGG
                     TAHLPTGPMRAAAMMGQRVIFMLGLYRGGNRYHVVFEPLADFSNVQRAQRQAAIEAAV
                     TRYAGLLEKYCRSDPYNWFNFFDFWRAPARAPSA"
     gene            11262..11855
                     /locus_tag="VNZ06_03960"
     CDS             11262..11855
                     /locus_tag="VNZ06_03960"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HWW19938.1"
                     /translation="MSAPRRLRLIAGAALLCVPAWRCAQGAPAADSAGALDQVMSLFA
                     QRRHAEASFTEEKYLSVLRHPISSSGRLIYEAPDHLEERTLTPRPQSVVLDHGLLSMK
                     IGQRLRTLRLADYPQLAPLIDSIRATLAGDRAQLERAFSLEFSGDAEHWQLHLRPLDA
                     HSPLTQIDLSGARDEVNEVRMQQRDGDHSVMRISSAE"
     gene            11852..14173
                     /locus_tag="VNZ06_03965"
     CDS             11852..14173
                     /locus_tag="VNZ06_03965"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWW19939.1"
                     /translation="MNTPPPITLWVWLLLVGLASWIVARAHYVADLSAFLPRSPSPTQ
                     QLLVQQLQDGPASRLILIGIEGADPLSRAAASRALAAALRAYPQFINVNNGEAGSEDQ
                     DRRVVFTHRYLLSSAITPEHFSIAGLSAAISDNLDLITSPAGLLAKSLFRSDPTGETL
                     QVIDQLESGRQLTGVEGVWSSADGQRALLLAQTRASGSDTDAQEQALTQLSAAFTRIA
                     AGSLRLLVSSPGKFAVDSRATIKQQAVRLSLLSTLLIATLLLWVYRSLPTLLFGLLPV
                     ASGALAGIAAVAVGFGIVHGITLGFGVTLIGESVDYSIYLFVQSRGAPLAAPRATLWP
                     TMLLGVATSVCGFASLLPSAFPGLAQLGLFSIAGLIAAAAVTRFVLPPLLPARVRMAD
                     LTPLGARCMRLLSHLHLPVTLWALLATGCVWVLYVDRGRLWNHDIAALSPIPDSAQRL
                     DAQLRADLDAPDVGSLVVVSADSEQAVLQLSEAVGRRLDALSADGIIGGYDSPARYLP
                     SERTQQARRASLPGDAPLRARLASVGDTLSLETAALEPFMEQVNAAREAPLLTRASLE
                     GSSFVQALDALLWQRDTHWHAILPLRAVNDLSQVRRALATLAPGQCQVLNIKQETDAL
                     YGGYLSEAIRLSLAGFGAIVLLLVLTLRSTRRVTRVILPLLLAVLMVATGLALLGVRM
                     SILHLVGMLLIVAVGSNYALFFDKRAHDCDRQTLPLTLASLLTANSCTVIGFGVLAFS
                     SVPVLSALGSTVAPGTLLALWLSALFAPRALWA"
     gene            14179..14832
                     /locus_tag="VNZ06_03970"
     CDS             14179..14832
                     /locus_tag="VNZ06_03970"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWW19940.1"
                     /translation="MQTIAHGAPGASTLRVVLMPGAYHTPNQFLDAGFAEAVRRRSLR
                     VELVLVVPELAHLTDRSWLTALHDEVIAPARAQGAPVWLGGVSLGGFMALRFAAQYPG
                     ALDGLCLLAPYLGSRIIAAQIAAQADAAAWQMHSLAEDDDERRIWHYIATLADSMNPT
                     RIFFGYGASDRFADTQRVLAQAVPATHATVRIIAGGHDWTVWRSLWDEFLDRYGGSA"
     gene            14829..15653
                     /locus_tag="VNZ06_03975"
     CDS             14829..15653
                     /locus_tag="VNZ06_03975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWW19941.1"
                     /translation="MNGASSSAVCVRWRASLLLRASVGLHIGAALLTVIRPHWWPWTL
                     SAVIANHLLLTALGLWPRSQALGSNWTSLPAAAAARGEVAITIDDGPDPVVTPQVLAV
                     LKRYEARATFFCIGQPAQRHAECVRECFAAGHAIENHSYQHRHNFSLLGPSGMQREIA
                     RAQQTLNEVSGSAPRFFRAPAGLRNPFLDPVLQRLQLQLVSWTRRGFDTVNGDADVVY
                     ARLVRGLKAGDILLLHDGHAARTTSGGPLILEVLPRLLETIRQHSLTPVTLRDALR"
     gene            15692..16897
                     /locus_tag="VNZ06_03980"
     CDS             15692..16897
                     /locus_tag="VNZ06_03980"
                     /inference="COORDINATES: protein motif:HMM:NF006618.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HWW19942.1"
                     /translation="MSPAVPLQLTRFTATSCLGSGLASSRAALEQQRSGLAPCRFESA
                     RLDTYIGEVPGVDDVKLPSRLQRFDCRNNRLALLGLQQDNFEAAVRTSAARWGHRRVG
                     VFLGTSTSGILSTEIAFRRLDPVTGALPGDFHYLGSHNSFSLVDFVRRYLELEGPAGT
                     VCTACSSSAKVFATAQRLIALDLIDAALVGGVDSLCLTTLYGFHSLQLTSAAPCRPFD
                     AARDGLSIGEAAAYALLERVPGSNAEPDSIRLLGVGESSDAYHMSSPHPEGTGARSAM
                     QAALTAAHATPAEIDYINLHGTGTPSNDSAEDRAVTALFGAATPCSSTKGATGHALGA
                     AGALEAVLCALTLQSGLMPGGLNTRQQDPRLKVNYLTANRRAPVRRVLSNSFGFGGAN
                     CALLFGRAA"
     gene            16910..17704
                     /locus_tag="VNZ06_03985"
     CDS             16910..17704
                     /locus_tag="VNZ06_03985"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HWW19943.1"
                     /translation="MTPLIASLQGVGVLGPGFDNWACTAAILRHEAAYQALATLLPAP
                     GVLPSAERRRAGRIIKLALAVGAEALAQAGADARTLPTVFSSSGGDGDNCHEICMTLA
                     SEDRQISPTRFHNSVHNAASGYWSIAYGCTRPSTSLCAGDASFGAGLLEAMAQLACGA
                     ESVMVLAYDADYPQPLRATRAIPDAFAIALLLAPAQSERALAQLQLELVGESPTTLND
                     PQLEALRLSIPAARGLPLLESLALRRAAVVYLDYLDSTRLAIGVSV"
     gene            17701..18168
                     /locus_tag="VNZ06_03990"
     CDS             17701..18168
                     /locus_tag="VNZ06_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19944.1"
                     /translation="MTQDQSVHDRTWIASHIPHQGTMCLLDRVISWDSQGIVCLCTSH
                     RDADNPLRAHGRLGAACAIEYAAQAIAVHGALLQPRAGAGIGLLASAREVELLVTRLD
                     DLSADITVSAQRLHSDTRGALYTFALHADHSLLVRGRASLLLSAEPAPGGAAP"
     gene            18165..18908
                     /gene="fabG"
                     /locus_tag="VNZ06_03995"
     CDS             18165..18908
                     /gene="fabG"
                     /locus_tag="VNZ06_03995"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HWW19945.1"
                     /translation="MNIAASATMRRALVTGGSGGVGAAICRQLVQAGHFVYVHAYRAR
                     AQAEALAQEIRAAGGQAEALCFDVTDESATHGAVEHALKAGPVQILVNNAGIHDDAVF
                     PGMTALQWQRVIAVSLQGFFHVTQPLMLPMLRTRWGRIVNVSSVAAQIGNRGQVNYAS
                     GKGALISATRALALEVATRGITVNAIAPGIIATRMSADSFDETAIARLVPMRRAGSPD
                     EVASLVSFLTGEGAGYITGQVVSINGGMY"
     gene            18924..20186
                     /locus_tag="VNZ06_04000"
     CDS             18924..20186
                     /locus_tag="VNZ06_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWW19946.1"
                     /translation="MTVQKQELLLYFTLLELAVIVIAGRLGDWVARGLGQSGVVGQIL
                     IGILLGPSLFGLISPGAFDYLFRTAPPEPMQILSSLGLVLLMFQIGMEFEFAHLTERA
                     NRRAVLSVAGICLAIPFILGVGFGYVVAPVLSPSANRLHSALFVATAFSITALPVLGR
                     MLIELDVTHTVLAVIAVSAAAINDVVGWLLLALITAITAGDFNGGGYGLRVALVAVYA
                     LICLIAVRPLVKAGVRRANPGERGLSANLIGALLALVFLSAIATYQLGIFAVFGSFMM
                     GVILFDERELVQAWRDTTGRFVNVFFLPIFFTYTGLRTSIGSLSSAADWAWCALTLGL
                     ACAGKFGGAYLAARLAGRTHAESTSLGYMMNTRGLMELVVLNVGYDLGVISPRMFTIL
                     VIMAVLSTAITIPALRRYLPRAGIALAA"
     gene            20233..20511
                     /locus_tag="VNZ06_04005"
     CDS             20233..20511
                     /locus_tag="VNZ06_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19947.1"
                     /translation="MTRQRTHSFSTALLLTALTLLQPVAALADHKPPKPGLLLGSHPS
                     KQAKAARLAVLKQRTSAVPLKPLHSPKVTNSLNHRAGKTMTVPQRPPK"
     gene            20618..20929
                     /locus_tag="VNZ06_04010"
     CDS             20618..20929
                     /locus_tag="VNZ06_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19948.1"
                     /translation="MNHRRLRLPAAMAVMALAAPVLAAGFGDNPGENPKADVQAAAAN
                     IQREAHAFGTSVRENSQQLHDQVAHSVHQFRHQFTIQWYRTGDSIHRWWNNTRDSFSR
                     S"
     gene            complement(20931..21800)
                     /locus_tag="VNZ06_04015"
     CDS             complement(20931..21800)
                     /locus_tag="VNZ06_04015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWW19949.1"
                     /translation="MSVMRWLGRSSPQGREDLNMRLTTLRIAGTSIWLALSGCASVSS
                     TVDSVKDKATQLAQVAKVHAQAVYERQQKYLAEHDTLKNFEDATEHSEAEVLKVLHAA
                     RKGSSANAPTPESPKAGPARPPQSSGSAPMLASAPMSAEGGMHWPVEAGVISSDYGAR
                     WGKLHKGIDIAADVGEPVYAVAEGDVIYAGDGLRGYGNVVIIQHGQRLSSLYAHNSQL
                     RVKQGDHVGQGMLIALIGSTGHSTGPHVHFELRQGDVAVNPRTLLPATTLADATTGVS
                     GSRGMLSTLNARR"
     gene            21872..22459
                     /locus_tag="VNZ06_04020"
     CDS             21872..22459
                     /locus_tag="VNZ06_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19950.1"
                     /translation="MQRQIDIAKRLDVPSLIALSLVAIGWFGMNTLVISVGRLVQATR
                     FYEVGVVILHPAALFLGVGSGHTFELTAFALLALLTLFAAMAAPVLFARRCAWLAGWA
                     PLVLMLVCAALLYGDGSSPPPPAADPGLRGDLMRFANQLLQHAQDTLVSHISVGAGGI
                     LSALASLIVAVRSTRRFRVESPEVAFAAAPSYGWT"
     gene            complement(22469..22972)
                     /locus_tag="VNZ06_04025"
     CDS             complement(22469..22972)
                     /locus_tag="VNZ06_04025"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWW19951.1"
                     /translation="MTNELVVARTFAATRELVFRAWSRAEHVKQWFSPEPYSVPEAEI
                     ELRVGGAFNVCMQSPQGQRHWSRGKFVEIVPNSRLVIDMHAVGDDNQPLFRAYTVVNF
                     VDLPGGATRIEVTQTYTLIDASMAAAMIGGAKLGWSQTLDKLEQELGRIKTLPAGRSG
                     SFGTFTV"
     gene            complement(22962..23315)
                     /locus_tag="VNZ06_04030"
     CDS             complement(22962..23315)
                     /locus_tag="VNZ06_04030"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWW19952.1"
                     /translation="MLNKSVALDRTFQALSDSTRRSILAQLSRRAISVSELAKPLAMS
                     LPAVMQHLAVLEHCGLVRTAKRGRVRTCRIEPQGLGLAEQWINARRAEWEAHFDRLGE
                     YLKTLKQMEEDPHDK"
     gene            complement(23370..24284)
                     /locus_tag="VNZ06_04035"
     CDS             complement(23370..24284)
                     /locus_tag="VNZ06_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWW19953.1"
                     /translation="MNQAPANDSLWLRVFASLPWGVLYAKAAVLAFLARYVFRFRVSI
                     ARDNLSRCFPQASAKQINTWLRAYYRQLGQIAVEFLKIGVMSAQQMRDHLSAIGFEQV
                     RAETAAGRSVILVAAHQCNWEWAFHGTVLQMNVPVDAAYKPLHGQKSDAQLRKLRVRY
                     GANLIAAKRLVREVISRRRQVQVHAVALLADQMPTSSQGRHWVSFLGRDTAFYPGPAE
                     IARMTGYAAFFIAMRRERRGHYTLEVIPICGANERLDPPIFTARYAALIEAQIRASPA
                     DWTWIHRRWKGERPADAALATTQASATH"
BASE COUNT         4040 a         7899 c         7853 g         4515 t
ORIGIN      
        1 ccaggggagc gagtcgatcg cgacgccccg cagcgcctca gggtggcatt gcgcgaaatg
       61 accgcgcgcg cacagcaccg attcgatgtg ctggccgccg ctgtcgagga aaatggtgaa
      121 tgccgcgtcc gccgaggtcg aatcgcggaa ggtctccaga ctgcgtcgga tgcaatcgtc
      181 cgcgctctgg cgcgtcatgt tctgcagatc gaccgctatc ttggtgcagc caacgtcgat
      241 gccaggaccc gttcgttctg ctctgccggt ccagaactgc gcagagagaa cgtcggacgc
      301 cgtaaagtcg gtagtagtgc ccggcggctt ggacatttgc cccaaattct cgcacaggtt
      361 gggtggaaca caagctaagc cgctgattta ttgacacacg tctaggatta gacggaattc
      421 gcagccaggg cggcacgcgc ggcttcgaaa cttgccttca gagcctgcgc catctcgagc
      481 tggagcgcct cgatttgctg gggcgtggcc atcttgggca cttcacgcga tggaccgata
      541 ccgatggcga tgcgcgtaaa cggcagcggt atcacgaaac gatcccaggc cacgagcttg
      601 gcacgggcgg cggcataggc gatcggcagc atcggacgct gcgacagctg cgacagcagg
      661 atcgctccgg gcttgaacac gaacttcggt ccccgcggac catccggcgt gatgataggc
      721 gagacctgtt gcttgatgag cgcctggaaa tagtccttca gcgcgagcgc gccggtatgc
      781 gaggaggatc cacggatcac gtagccgccc agctttcgta ccatcatggc gcccagctcg
      841 ccatccaccg acggactgat cagaaatccg gcgcgcagac gaccctgctg ttgcagcatc
      901 agcaggaagc gtgcgccgaa cagctggtgc tgatgccagt agcacggcag gaacgagggc
      961 cagcgcgcca gcaccgcatc gagatgctcc tgaccgctga tcgcgacgat ccggcagctg
     1021 cgccaccagg tgcggattac cgccagcagg atgggtgcgg ccagccggta cagcagacgc
     1081 cgggtcggag tcatgcgcct gcctgacacc gtgacccggc gatagatcgt attgcgcagc
     1141 gtgccgtcgg agggttcgtc gttcacgtca gcgccgattg taagcccttg ggcgcgcaag
     1201 cgcgtgcgct agaatcgtag ccatccacat ggcaccgtta ctcgaagccc tgcgcaacca
     1261 ttgatctgcc catcatgagt tcaagccctc tgtacacggc cacgacgctg actgccctga
     1321 tcgaaagcct gcgcgaaaac agcagcggtg gccttcacta ccacgacagc gagcgcgaaa
     1381 cgcgccgtgt gtcgttcgcc gagctctacc agcgcgcgct cggtattttg tatcacctgc
     1441 agcgcctggg tgcccggccg ggcgacaagc tgatcctgct gctgtcgggg aacgagccgt
     1501 tcatcgacgc cttctgggcg gcgatgctgg gcggcatcgt tccggtaccg atcgctcccg
     1561 gcatcagcga cgagcaccgc cacaagctgc tgcgtatcgc gcgcaagctc cacgacccgt
     1621 tcatctatac ggaacaacgc ctgctggagc gagtcgcagt actggctgcg gccagcggcg
     1681 aggctgcacg caccaccttc gaggcgctgc gtgcacgcgc attcctggtc gatcagctcc
     1741 atgacatcag ccgggccggc aagctgcacc gcgcgcttgc gagcgacacc gcgttcatcc
     1801 agttctcatc aggctcgacc agtgaaccca agggagtggt gctcactcac gcgaatctgc
     1861 tggccaacat acgagccatt ggcttcgcgg cgggactgaa cgccagcacc atcacgctct
     1921 cctggatgcc actgacgcac gacatgggac tgatcggctt tcatctggtg ctgctcggcc
     1981 acggggcgaa cgcgcacctg atgcccacgg aactgttcgt ccgacggcca ctgctgtggc
     2041 tggccctcgc gtcgcgcgtg cgcgccacca tgctgtgctc gccgaacttt ggttaccgcc
     2101 actacctgaa aatgctgggc gaacgccgca tcgaagacct ggatctgtcg agcgtgcaac
     2161 tcatctacaa cggcgccgag cccatcagcg tcgatctgat ggaagaattc ctcagtcgaa
     2221 ccgcgccggc gcggctggcg cgcaacgcca tgtttccggt atatggactg gcggaagctt
     2281 cgctcgcggt cagctttccg cagcccgggg cgccctctca ggtgctgtcg ctcaatcgcc
     2341 atcagctcaa cgtcggtgaa aagccgcaga ccgacggcgt cgccgaacgc gacgtgctgc
     2401 gcctggtggg cgtgggtcgc gcggtgccgt actgcgagct tcgcatcacc gacgatcggg
     2461 acctgccgct cggagagcgc caggtcggac atatccagat ccgcggcgac aatgtcaccg
     2521 gcggctactt cgaggacccg gagctcaatc gctcgagctt cagtgccgac ggctggctgc
     2581 gcaccggcga tctggggctc attcatcgca cccagctcta catcaccggc cgtcacaagg
     2641 aaatcctgtt cgtcaacgga cagaactact acccgcacga tctggaaggc ctgctcgagc
     2701 aggtcgaggg gctggaactg ggcaaggtgg tggtcgccgg ggtgcggccg ccgggggcac
     2761 aggaggacga gctgctggtg ttcgtgctgc accggggcac gctggccgat ttcatgccac
     2821 tggcacgcga ggtcgctcga cgcattgccg agagcgcagg ccttgaagtc agcgccgtga
     2881 ttccggtcaa acggattccc aaaaccacca gcggcaagat tcagcgccat gcgctcgaaa
     2941 ccgagtacct caccggcgca tttgccgccg aactcgcaca gatcggcacg ctgagggcca
     3001 cgcaggcgaa gcctgcggtc agcggcagcg acattgagca ggcactgcgc gatatctgcg
     3061 aacaggaact cgagggccgc agcatcgatc gcgacgagag cctgttcgac atcggcgcca
     3121 gttccatcaa gctgctggcg attcatcagc gcctcgagca gcagtggcct gggcgggtgg
     3181 acgtgaccga tatcttcgat cacccgagca tcgcagcgct ggcgcgcttc atcgagcaga
     3241 gcctgacagt gcgggagcag gtatgagcga tcgtctgcag cgcgcggcgc aatatctggc
     3301 ggccctgcgt caggccggcc cgcgacccgt gagcctgccg caggagctcg cccctcaaaa
     3361 cgcagcgcag gcctatgagc tgcagcagcg cgtcgcaaga gagttgaagg tcagcgccgg
     3421 tggctggaag gtggcgatga gtgggctcaa cgccggcagc tacgcgccgg tgttcgcaac
     3481 cgacctgcat gccagcggcg cccgggtcac ctcggcgatc agccaacagc tcggcgtcga
     3541 accggaagtg gcgttcacat tgaagcgcac gctgcctgcg gggcggcgct acacccgcga
     3601 gcaagtgatc gaggcgatcg gcagcgcgca tgctgccatc gagatcgtga tcagccggtt
     3661 tcagcggcat gagaccgccg cagtgctgga tcgactggcc gacaacatca gcaacggcgg
     3721 gctggtctta ggtcccgccc ttgatcgctg gcagcacctt gatttcagtg cgttgccctt
     3781 gacgctcact ctgaccgccg ccaacggcga agcgcgtgtg catcagtccc gcggtggaca
     3841 cccacaggcc gatccgctgc tgccgatgac ctggatcgtc aatgaacgcg ccgcgctcgg
     3901 cttcgggatg cgtcaggggg aggtggtcac caccggcagc ttcgccggct tgcattatgc
     3961 agcgcgcggc acgcgtgttc gggtcgaatt cgaagggctg ggcggcgccg agctctacgg
     4021 ctaaaatccg gcacttatgc ctcaacaggg agcgatcagg gcggcggcgc ggtgcagcgg
     4081 cacgcggcat aggaacgtgt tcgtgcattg atgggctcag ctgcggcgct tcaagtcgaa
     4141 tatccgcgtc tggatgagat ccgccagcgc ccggctgcct ggtggcagga cgtgctggct
     4201 ctcattcatt tcaatgacgg tgagtgctca cagccgcagc tgccggcggc cgtgaacatt
     4261 ccagtggctc aggtgcgcgt gccgatgctc gccggcgctg acggtgcggt cgaagtctgg
     4321 cgtctgagtg gtcccatgcg ctccggccag tccggacgtg tcgcctatcg ccacaacggt
     4381 cgcttgctgt tcgcctcatt gagtatttcc gagcaggaat ttcccggtgg cagcgacgcg
     4441 gactcggcca cggcccttcg ctgcgccacg gaagccggct atagcgaact attcagcacg
     4501 atcgaggcac tgcattttcc gcatccgctg cgcatctgga atttcctgcc agcgatcaat
     4561 ggtcaaagcg aaggtggcga gcgctactgg catttcaacg gcgcgcgaca ggacgtgttc
     4621 ctcggcttca atcgctgcat caccggcaac gtgcctgccg ccagcgcggt cggtgcggtc
     4681 tccacctcgc cgctgaccat ctattgcatt gccagtgcca gtgcaccgat ctcgctggag
     4741 aatccgcgtc agcgcgccgc ctgggcctac ccaccgcagt acgggccgaa aagcccgacc
     4801 ttcgcgcgtg cctgcatcga gggcgacaac gcccacacgc tgtttatttc cggcacctcg
     4861 agcatcatcg gtcatgaaag cgcacacgcg ggggatgagt gcgaacagac gcgtgagacg
     4921 ctgcgcaaca tccgtgccct gatcggggcc gcgaacgagc gcatcggcgc gcagcgcttc
     4981 agcctgcctg agctgcacta caaggtgtat gtgcggcatc cggcgcacct gtcaaacatc
     5041 gaacgtgaac tgcgacgcga gatcggcgag cggccggcgg tgatgtacgt gcaagccgac
     5101 atctgtcggc gcgaactcat ggtggagatc gaagccgtag gcacgatggt gtccgggtga
     5161 gcggccagcg cgctgcggcc gcagcgctgg gcctgctggg tgccctcacc tgtggcagcg
     5221 cctgcgccga ccctcaaccc ctatgggact tcggtctggg ggtgggggcc gtggtcttca
     5281 acgactatcg cggcgcttca gcaatgcacg gctatccggt acccgtgccg tactttatct
     5341 atcgcggggc gttcctgcgc gctgaccgcg acggggtaca tgggcggttt cttgattcgc
     5401 tgcatcttga gttcgacatc agtgcggacg cgaccccgcc ggtcttcagc caccacagtg
     5461 cgccgcgtca gggcatgccg aatctggcct cgaccgtgga actcggcccg tcgctgacct
     5521 ggcatgcctg gcgctcgcag gaccaacggc tgcgcttcga tgtgcgctcc ccggtgcgca
     5581 acgcgattac gatcgattcg cccccccgat cgatcggctg gatctacgcg cccaacctca
     5641 gcgtggacta cctcggcaag ggtcgcgcaa ggggctggaa tttcggcctg ctcaccggcc
     5701 cgctatacag tcagcggcaa tacaaccgtt attactatgc tgtggccccg caatacgcca
     5761 ccgcgagccg cccggcgtat caaccccccg gcgggtacgc aggcactcag ctgttggttt
     5821 ccaccagcaa gcgctaccgt gactactggt tcggggccta tttgcgccat gattggcttc
     5881 agggtgccgc gttcgatccc agtccgctgg tgcagcagca cagctactgg tcgggaggat
     5941 tcggtttcgt gtggatgatc cactcctcgt ccagaatggt ggacagcgat gaatgaagtg
     6001 cgcgcggtgg gcgtcacccc accatcgaag gccgcaaaga ctctgctcga atacctcatt
     6061 ctctatagtt cgctgctgct gctggcgctg atctgtctga cctggtcgct gctggcattg
     6121 ccgctgtatc tgctgctgcc gcgacgcgcg gcgattgctt tcggccgccg cggcatcatg
     6181 gctggcttcc gtctgtacgc gtactggacg gcagcggtcg gcgcctatcg cctggatctg
     6241 accgcggttg actcgctgcg cggtgctccc ccgatgattc ttgctccgaa tcacccctcg
     6301 ctggtcgatg cgattctgat tctcacgcgc catccgaatc tggtgtgcgt gatgaaatcc
     6361 gatctgatgg agaacatcct ctacggcccc ggagcgcgtc tggcgcgcta catcaacaac
     6421 agctcgccgc tgcgcatggt gcgcgaagcg gtcgccgccc tcgaagacgg cggcacactg
     6481 ctgctgttcc cggagggcac gcgctcgtcg aaatctccga tcggtgagct gaagagaagt
     6541 atcgcggtga tcgcccgcca ggcgcaggtc ccggtacaga cgctgatcat cgaaatcgac
     6601 cagcccttcc tcagcaaggc gcgcggtcta tggtcacgcg catcctttcc ggtcacttat
     6661 cgggtacgtc tcggcaagcg ctttgatccg cctgaagacg tggagacgtt catggccgaa
     6721 ttggaacgtt attaccgcag cgagctgcag ggcagcctcg tggcggactg gctcagcaga
     6781 tcaccgtgcc cgtagcgacg gcttcgacga cacatctggt cctgattccc agctacaacc
     6841 ccggtcccaa ggtatttgag acggtcacgg cggcgcgcga gcattggaat ccggtctggg
     6901 tcgtggtgga cggcagcatt gacggcagcg cggtcgcgct cgcggcgatg gcgcagcacg
     6961 acccggggtt gaaagtgctg accttgccga agaaccgggg taagggggca gcggtggcac
     7021 acggcgcccg ccgggcgcga ttggccggct tcacccacat tctgacgatg gactcggacg
     7081 gacagcaccc cgccgatcac atcgagcgtt tcatgcaggc ctcggtcgca gaaccgcagg
     7141 cactggtgct cggcgatccg gtgttcgatg ccagcgcccc ggcgctgcgg gtcggtggtc
     7201 gcaaggtctc gaacctgttc gccaatctgg agaccctctg gctcggcatc caggactcgc
     7261 tgtttggatt tcgtgtctat ccgatcgacc cactgctgaa ggtgatgcgc cgacagccgt
     7321 ggatgcgccg tttcgacttc gacgtcgaag ccgcggtgcg gctggtgtgg cggggactgc
     7381 cgccgcgcaa cctgaaggcc ccggtgcgct acttccggcc ggaggaaggc ggggtctcgc
     7441 acttccatta cgggcgcgac aacgctttgc tgagctggat gcatctgcgg ctgttcatcg
     7501 ggttcctgct acgccttccg gtgctgctct ggcgacgtgt aaacgtctga aatcgatcgg
     7561 aaacaacacg ctcggattca tgtgacaatc tcgatataat gcgccgcaac ttggcttacc
     7621 cccggcgcgc cggccacaaa atctgagagc gcccggaccg gagagcgttg ccccaatcat
     7681 gtccgaacaa gcgttgccaa ctcagaccgc gccgcgtagc gattcagtcg tgctggagcg
     7741 cgaaattgcg cggctcattg tcaagggact gaatctgccg gtacaggccg aggacatcga
     7801 tccggaagca cccctctatg gcgaaggcct ggggctggac tcgatcgata tcctggagat
     7861 cgcgctgctg gtctccaagc agtacggcat tcaattgcgg gccgataacc aggaaaacga
     7921 gcgcatcttt cgttcgctgc ggcacttcgc gcagtacgtc gctgcgcaga gaaccaagtg
     7981 aactatcgct ggctgcgcca tggactggtc gcggcatcga ttctcacgta cgcactgctg
     8041 cagcactacg ccaacaccac acccggcgcc gcagctcttg gggcaggact ggcgatcaca
     8101 ccgctgttga tcacctggct gctgctggcc cggcgcacgc cgtggccgcg gatcggcgtg
     8161 ctgctggcct cgctgctggc cgccgcgctg ctggctgaga gctggtcgcg catcgaacat
     8221 agttttccgc tcatgttcat gttgcaggaa gcgggcgtct atctgatcct cgcattcggt
     8281 tttggtcgga gccttatgtc gggacagaca cccttgtgca cgcactgggc gaccaggatc
     8341 cacgggccat tgtccgagcc ggtggagcgc tatacccgcg cggttaccct cgcctggacc
     8401 gtgttcttcg ttgctatcag cctcaccagc gtgctgctgt atgcgttcgc ggcgctgcgc
     8461 gtctggtcta tcttctccaa cttcctcacg ctgccattgg tcgctctgat gttcgcgatc
     8521 gaatacgaag tgcgccgtca ccgcttgccg tggatgcagc gcgcgagcct tgccgatacc
     8581 gcgcgcgcct atttcgcgac gaccacccgc ggtcgcccga tctgaaggac ccgacacgca
     8641 tggcgatgct gcctctgctc gcgcacgacc ggcccgatgc catcgtcgct taccgcggtg
     8701 gcgagcccat cagcgccgcg cagtttctgg cagacacgcg gacgctcgcg cgacgcctgc
     8761 ccgccagcac gcacattctc aatgcgtgca aggatcgcta tcacttcacg gtaggactgg
     8821 gagccagcct gctggccggc aaatgcagtg tgctgccttc cacccatacg cccgaggtca
     8881 tccggcacct gcgcagcttc gcgccggatg tgttctgcct gagtgatgat cctcagaact
     8941 cgatcgacct gccccagctg ttgtatacgc ccgacgccac gaaagctcag ttgcgatccg
     9001 gcgccggctg gaacgtgccg cagatccacg ccgagcggct ggccgcgtac atcttcacgt
     9061 ccggttcgac cggcgtgccg ctcccgcatc gaaagacctg gggacgactg gtcagttgcg
     9121 tgcgcgcgga agtctcgcgg ctgacaccgc tgcagcgtac gccctgcgcg atcgtaggca
     9181 cggtgccgcc gcaacacatg tatggatttg aatccacggt gttgctgccg ctgcagagtg
     9241 gcagcgcact gagcgccgag cgtccgttct atccggcgga cattgccgag atgctcgagc
     9301 gtctgcgcgc gccgcgcgtg ctgttttcga cgccggtgca cctgcgcgcg ctgctggcat
     9361 ccgacatccg tctgccgaaa ctggagctga tcgtctcggc gaccgcgatg ctgtcggcca
     9421 atattgcgcg cgaagtcgag cagcggttcg gctgtgagct gctggaaatc tacggctcga
     9481 ccgaaaccgg acagatcgcc acacgtcgca ccgctaccga ggttcgctgg cgactgtggc
     9541 ccgacgtcag gttgcaggta tcggacagcg gcgccgccgc ttacggcggg cacgtgggtc
     9601 agcccacgcc actggccgat gtggtggaga tcactgaccc gcagcacttt ctgctgcacg
     9661 gtcgcacggc tgatctggtc aacattgccg gcaagcgcaa ctcagtcagc tacctgaatc
     9721 atcagctcaa cgcgattcct ggcgtgctcg acggcgcatt cttcgtgcgc gaggacaccg
     9781 atgagtcgat cgccggcagt ggccggctcg ctgccgtcgt ggtcgcaccc gggctcacgg
     9841 cgagcgcgat cactcaagca ctgcgtgagc gcatcgatcc ggtgtttctg ccgcgaccgc
     9901 tgctgctggt cgagcaactg ccgcgcaacg aaaccggcaa attgccgcag cacacgctgc
     9961 gcgagatcgc cgccgaagcg gccgtcgcgc agccgcatct gttcgtatga cgcgtcaaag
    10021 cctggcagtg cgcatcgccg cggatcatcc ctccctggcg ggtcattttc cgggattccc
    10081 gatcgtgccg ggcgtggtgc tgcttgatga ggctctgcac gcgatcgaac agctgcaggc
    10141 agccgaaccg gcatcgtggc acatcacgac cgtcaagttt catcacaccc tcgcgccggg
    10201 cgagccattg cagctcagcg tcgagtgtca ctccgatgca cgggtgcatt tcgagctgcg
    10261 cagtgcacgg gcgctggtgg ccagcggaac gatcaaacgc cgcgcccgaa ttcgtgcggt
    10321 ggtatcgggc cggtgaatac ctcggtgacg acgcgcgcgg ccgagtggcg tcgcagtccg
    10381 gagcgcggca gccgcgcgct gctgcgggcg atgatgtggc tgtcgctgcg gttgggcagg
    10441 cccgccggac gagtgatcct gcacggcatc gcggcctact tctttttgtt cgcgccgaaa
    10501 gcgagtcagt cgatgcggca gtatctgcgg cgggtactgt cgcgcaagcc gcgcgccagc
    10561 gaccgctgga agttgatcct caatttcgcg accatgattc atgaccgtgt gtacctgctc
    10621 gccgagcgct accacttgtt cgatgtgtcg ctgcagggcg aggcgatgat gaaagaactg
    10681 gtcgccgccg gcggcggcgc gatgctgatg ggtgcgcatg tcggcagctt cgagatcctg
    10741 cgctggattg gcgagcgaca accgaacctt cgcatcacga tcacgatgta cgaggaaaat
    10801 gcgcgcaagc tcaatgaaat tctggcggcg atcgcaccgc agaatccacc ggacgtgatc
    10861 gcggtcggcc atctggacgc gatgctgcgc atccgtgagc gcctggacga aggcgcgctg
    10921 gtcggcatgc tggcggaccg cactttcggc gacgaagcca cattgccggt gacctttctc
    10981 ggtggcaccg cccaccttcc aacgggcccg atgcgagccg cggcgatgat gggtcagcgc
    11041 gtgattttca tgctgggact ctatcggggc ggcaatcgct atcacgtggt gttcgagccg
    11101 ctcgcggatt tcagcaacgt ccagcgcgcg caacgccagg cggcaatcga agcggccgtt
    11161 acgcgctatg cggggctact ggaaaagtac tgccgcagcg atccttacaa ctggttcaac
    11221 ttcttcgact tctggcgcgc gccggcgcgc gccccgagcg catgagtgcg cctcggaggc
    11281 tgcgcctgat cgcgggcgcg gcgctgctgt gtgtgcccgc gtggcgctgc gcacagggtg
    11341 ccccggcggc ggatagcgct ggcgcactcg atcaggtgat gtcgctgttc gcgcagcgcc
    11401 ggcacgctga ggccagcttc accgaggaga aatatctctc ggtgctcagg catccgatca
    11461 gctccagcgg ccgactgatc tatgaggccc cagaccatct ggaagagcgc acactgacac
    11521 cccgaccgca aagcgtcgtg ctcgatcatg gcctgttgtc gatgaagatc ggtcagcgac
    11581 tgcggaccct gcgcctggcg gactacccac agctcgcgcc actgatcgac agcattcgcg
    11641 cgaccctcgc cggtgaccgt gcgcagctcg agcgggcctt ctcgctggag ttctccggcg
    11701 atgccgagca ctggcaactg catctgcggc cgctcgatgc ccacagcccg ttgacgcaga
    11761 tcgatctgag cggcgcgcgc gatgaggtca acgaagtgcg gatgcagcag cgcgacggcg
    11821 atcattcggt catgcgcatc tcgagcgccg aatgaacacc ccgcctccga tcacgctgtg
    11881 ggtgtggctg ttgctggtgg ggctcgcatc gtggatcgtc gcccgggcgc actacgtcgc
    11941 tgatctgtcg gcgttcctgc cgcgctcgcc ctcgccgacg cagcaactgc tggtacagca
    12001 gctgcaggac gggcctgcct cgcgcctgat cctgatcgga atcgaaggcg cggatccgct
    12061 cagccgggcc gccgcctcgc gagcgctggc cgcggcattg cgggcatacc cgcagttcat
    12121 caacgtgaac aacggcgagg ccggcagcga agatcaggat cgccgcgttg tcttcacaca
    12181 ccgctatctg ctcagcagcg cgatcacgcc ggagcatttc agtatcgcag gtctgtcggc
    12241 cgcgatctcc gacaacctgg atctgatcac ctcaccagcc gggctgctcg ccaaatcgct
    12301 gtttcgttcc gatccgactg gcgagacgct gcaggtcatc gaccagctcg aaagcggccg
    12361 gcagctgacc ggtgttgagg gagtctggag ttccgcggac ggtcagcgcg ccctgctgct
    12421 ggcgcagacc cgggccagcg gctcggacac ggacgcacag gagcaggcct tgacgcagct
    12481 cagcgccgcc ttcacgcgca tcgcggccgg ctcgctgcga ttgctggtca gcagcccggg
    12541 gaagttcgcg gtcgattcgc gcgccacgat caagcagcag gcggtacgac tgtcgctgct
    12601 gagcacactg ctgatcgcga cactgttgct gtgggtctac cgatcgctgc cgaccctgtt
    12661 gttcggcctg ttaccggtag cctccggggc actggccggg atcgccgccg tggcggtggg
    12721 ttttggcatc gttcacggca tcacgctcgg attcggtgtc acgctgatcg gtgaatcggt
    12781 cgactactcc atctatctct tcgtgcagtc gcgcggtgca ccgctcgcgg cgccgcgcgc
    12841 aacgctgtgg ccaacgatgc tgctgggtgt cgccacctcg gtgtgcgggt ttgcctcgct
    12901 gttaccgtcc gcgtttcccg gactggcgca gctcggcctg ttctcgatcg caggtctgat
    12961 cgccgcggcg gccgtcacgc gcttcgtgct gccgccgctg ttgcccgcac gggtacgcat
    13021 ggccgatctg acacccctcg gtgcgcgctg catgcgcttg ctgtcgcacc tgcatctgcc
    13081 ggtcacgctg tgggcactgc tggcgacggg ctgcgtgtgg gtgctgtacg ttgaccgtgg
    13141 ccggctctgg aatcatgaca ttgcggcgtt gagcccgatc cccgatagcg cgcagcgtct
    13201 ggacgcgcag ctgcgcgcag atctcgatgc gcccgacgtc ggcagcctgg tggtggtgag
    13261 cgccgatagc gagcaggcgg tgctgcaatt gagtgaggcg gtcggccggc ggctggatgc
    13321 cctgagtgca gacgggatca tcggcggcta cgatagcccc gcgcgctatc tgcccagtga
    13381 gcgcacgcag caggcccgcc gcgccagcct gcctggcgat gcgccactgc gagcgcgact
    13441 ggcctccgtc ggcgacacgc tgtcgctcga aaccgcagcg ctcgagccat tcatggaaca
    13501 ggtgaacgcc gcgcgcgaag ccccgctcct gacacgcgct tcgctcgaag gctcctcgtt
    13561 cgtacaggcg ctcgatgctc tgctatggca gcgcgatacg cactggcatg cgatcctgcc
    13621 attgcgcgcc gtcaacgatc tgtcgcaggt tcgacgggca ctggctacgc ttgctcccgg
    13681 acaatgtcag gtgctgaaca tcaaacagga gaccgatgct ctgtacggcg ggtatctcag
    13741 tgaggcaatc cggctgtcgc tggccggatt tggcgcgatc gtgctgctgc tggtgctgac
    13801 attgcgcagc acgcgccgcg tgacgcgcgt gatcctgccg ttgctgctgg cggtgttgat
    13861 ggtggcgacg ggactggcgt tactgggagt gcgcatgagc atcctgcatc tggtgggcat
    13921 gctgctgatc gtcgcggtcg gctccaacta cgcgctgttc ttcgacaagc gtgcgcatga
    13981 ttgcgaccgc cagacactgc cgctgacgct ggcctcacta ctgactgcca atagctgcac
    14041 cgtgatcggc ttcggtgtac tggccttctc gagcgtaccg gtcctgagtg cgctgggctc
    14101 caccgtggca ccgggtaccc tgctggcact gtggctgtcg gcgctgtttg cgccgcgtgc
    14161 actatgggcc tgatctccgt gcagaccata gcgcatgggg cgccgggggc ttcgaccctg
    14221 cgcgtggtgc tgatgcccgg cgcctatcac actcccaacc agtttctgga cgcgggcttt
    14281 gccgaagccg tgcgccggcg gtcgctgcgc gtggagttgg tcctggtggt gccggagctg
    14341 gcacatctga ccgatcggag ctggctcact gccctgcacg atgaggtgat tgcaccggcg
    14401 cgtgcgcagg gtgcgccggt gtggctcggc ggtgtatcgc tcggcggctt catggcgctg
    14461 cgctttgccg cgcaataccc tggcgcgctg gacggactgt gtctgctggc accctacctg
    14521 ggcagccgga tcattgcggc gcagatcgcg gcgcaggcgg acgccgctgc gtggcagatg
    14581 cacagtctgg cggaggatga tgacgagcgg cgcatctggc actacatcgc gaccctggcc
    14641 gacagcatga accccacgcg cattttcttc ggttatggcg cgagcgatcg tttcgccgac
    14701 acccagcgcg tgctggccca ggccgtgccg gcgacacatg cgaccgtgcg gatcatcgcc
    14761 ggcggccacg attggacggt ctggcgctcg ctgtgggatg agtttctgga ccgctacggc
    14821 ggttcggctt gaacggcgcg agctccagtg cggtctgcgt gcgctggcga gcctcgctgt
    14881 tgctgcgcgc ctcggtggga ttgcacatcg gcgccgcact gctgaccgtg atccgtccgc
    14941 actggtggcc gtggacgctg agcgccgtga ttgccaatca cctgctgctc acggctctgg
    15001 ggttgtggcc ccgcagccag gctctcggct cgaactggac ctcgctgccg gccgcagcgg
    15061 cggcgcgtgg cgaggtcgcg atcacgatcg acgatggccc ggatccggtg gtcaccccgc
    15121 aggtgctggc ggtgctgaag cgatacgagg cccgtgccac ctttttctgc atcggccaac
    15181 ccgcacagcg tcacgccgag tgtgtgcgcg agtgcttcgc ggcgggccac gccattgaga
    15241 atcatagcta tcaacaccgg cacaattttt cgctgctggg accctccggg atgcagcgcg
    15301 agatcgcgcg cgcccagcaa accttgaacg aggtgagcgg cagcgcacca cgtttctttc
    15361 gtgcgcccgc gggcttgcgc aatccgttcc tcgatccggt gttgcaacgg ttgcagctgc
    15421 agctcgtgag ctggacacgg cgcggatttg ataccgtcaa cggcgatgcc gatgtggtgt
    15481 acgcgcgcct ggtccggggc ctcaaggccg gcgatattct gctgctgcac gacgggcacg
    15541 cggcccgtac cacgagcggg ggacccttga ttctggaggt tctgccgcgg ctgctggaga
    15601 ccatccgaca gcactcgctg acgccggtca cgctgcgcga cgccctgcgg taagcgctgg
    15661 aggtggccga tcctctagta tgcttgcggc catgagtcct gcggtaccgt tacagttgac
    15721 gcgctttact gccaccagct gccttggcag cggcctcgcc agttcgcgcg ccgcgctgga
    15781 acagcagcgc agcggcctgg cgccgtgccg cttcgagagc gcgcgcctcg atacctatat
    15841 tggcgaagta ccgggagtgg acgacgtcaa gctaccttcc cgattgcagc gctttgactg
    15901 ccgcaacaat cgcctcgcgt tgctgggact gcagcaagac aacttcgagg ccgcggtgcg
    15961 gaccagcgcg gcgcgctggg gacaccggcg cgtcggcgtg tttctgggca ccagcacctc
    16021 aggaattctc agcaccgaga ttgcgttccg gcgtctggat ccggtcaccg gcgccctgcc
    16081 gggcgatttt cactatctgg gatcgcacaa ctcgttttcg ctggtcgatt tcgtccgccg
    16141 ttacctcgaa ctggaaggac ccgccggcac ggtgtgcacc gcctgctcct ccagcgccaa
    16201 ggtcttcgcg accgcgcagc gcctgatcgc cctcgatctg atcgatgcgg cattggtcgg
    16261 cggcgtggat tcgctgtgtc tgaccacgct gtatggcttt cactcactgc agctgacgtc
    16321 ggctgcgccg tgccgcccgt tcgatgcagc gcgcgatggc ctgtcgatcg gtgaggccgc
    16381 ggcgtacgca ctgctcgaac gcgtcccggg ctcaaatgcg gaacccgatt cgatccgcct
    16441 gctcggagtc ggcgagtcga gcgatgccta tcacatgtcc agcccgcatc cggaagggac
    16501 tggagcacgc agcgcgatgc aggccgcgct gaccgccgcg catgctacgc ccgcagaaat
    16561 cgactacatc aatcttcacg gtaccggcac tcccagcaat gacagcgccg aggatcgcgc
    16621 cgtcaccgca ctgtttggcg cagcgacccc gtgcagctcc accaaggggg ccaccggtca
    16681 cgcgctcggt gccgctggcg cgctggaagc ggtcctctgc gcgctgacgc tgcaaagcgg
    16741 gttgatgccc ggcgggctca atacccggca gcaggaccca cgactgaagg tcaattacct
    16801 gactgccaac cggcgtgcgc cggtgcgccg cgtgctgagc aattcgttcg gctttggcgg
    16861 cgccaactgc gcgctgctgt tcggccgtgc agcctagggc atgcccgcca tgactccttt
    16921 gatcgcctcg ctacagggcg ttggcgtgct gggacccgga ttcgacaact gggcttgcac
    16981 cgcggcgatc ctgcggcatg aggcagcgta tcaggcgctc gcgaccttgc tgccggcccc
    17041 aggtgtcctg ccgagcgccg agcgccggcg cgccggccgg atcatcaagc tcgcgctcgc
    17101 cgtgggcgct gaagcgctcg cgcaggcggg cgccgatgca cgcacgttgc cgacggtgtt
    17161 cagctcctcg ggcggtgatg gcgacaactg ccacgaaatc tgcatgacgc tggccagtga
    17221 ggatcgccag atctcgccta cccgctttca caattcggtg cacaacgcgg cttccggcta
    17281 ctggagcatc gcctacggat gcacgcgtcc ctcgacctcg ctatgcgccg gcgatgccag
    17341 tttcggcgcc ggtttgctcg aggcgatggc gcaactggcc tgcggtgccg aatcggtgat
    17401 ggtgctggcg tacgacgccg actacccgca accgctgcgc gccacgcgcg ccatccccga
    17461 tgcgttcgcg atcgccctgc tgcttgcacc cgctcaaagc gagcgggcgc tggcacagct
    17521 ccagctcgaa ctggtcggcg aatcgccaac cacgctcaac gatccgcagc tggaagcgct
    17581 gcggctttcg atcccggcgg cgcgcggtct gccgttgctc gagagtctgg cgctgcggcg
    17641 cgccgcggtg gtgtacctgg actacctcga cagcacccgc ctggcgattg gagtgagcgt
    17701 ttgactcagg atcaatcggt tcacgatcgc acctggattg cctcccacat ccctcatcag
    17761 ggcaccatgt gcctgctcga tcgggtgatc tcgtgggaca gccagggcat cgtgtgcctg
    17821 tgcaccagcc atcgcgacgc tgacaatccg ctgcgcgcac atggccggct gggagccgcg
    17881 tgcgccatcg aatatgccgc gcaggcgatc gccgtacacg gtgccctgct acagccgcgc
    17941 gctggcgcgg gcatcgggct gctggcgagc gcgcgggaag tcgaactgct ggtcacgagg
    18001 ctggatgatc tgagcgccga tatcacggtg agcgcacagc gcctgcacag cgatacgcgc
    18061 ggagcgctgt atacgtttgc gctgcatgcc gatcacagtc tgctggtgcg gggccgtgcg
    18121 agtttattgc tcagcgccga gcctgcaccc ggcggtgcgg cgccatgaac atcgctgcca
    18181 gcgcgacgat gcggcgtgcc ctggtgacgg gcggcagcgg aggggtgggc gcagccatct
    18241 gtcgccaact ggtgcaggcc gggcacttcg tctatgtgca tgcgtaccgc gcacgcgcgc
    18301 aagcggaagc gctggcacag gagatccgcg ccgctggcgg acaggcggag gcgctgtgct
    18361 ttgacgtcac cgacgagtcg gcgacgcatg gcgcggtcga acacgcgctg aaggcagggc
    18421 cggttcagat cctcgtcaac aacgccggca ttcacgacga cgcggtcttc cctggcatga
    18481 cggcgctgca atggcagcgc gtgatcgcgg tttcgctgca gggattcttt cacgtgacgc
    18541 agccgctgat gttgccaatg ctgcgtaccc gctggggccg gatcgtgaac gtgtcttcgg
    18601 tggctgcgca gatcggcaat cgcggccagg tgaactacgc ctccggcaag ggcgcgctga
    18661 tttccgccac ccgcgcgctg gcactcgaag tggccacccg tgggatcacc gtcaacgcga
    18721 tcgcccccgg aatcatcgcc acccgcatga gcgcagacag tttcgatgag acggcgatcg
    18781 cgcgtctggt gccgatgcgg cgtgccggca gcccggacga agtggccagc ctggtcagct
    18841 tcctgaccgg agagggtgcc ggctacatca ccggccaggt cgtgtcgatc aacggcggca
    18901 tgtactagcg cggatcgcga acggtgacgg tgcagaagca ggaattgctg ctctatttca
    18961 cgctgctgga actcgcggtg atcgtgatcg ctggacggct gggggattgg gtcgcacgcg
    19021 ggctgggaca gtcgggagtc gtcggacaga tcctgatcgg gatcctgctc ggtccgtcgc
    19081 tattcggatt gatcagtccc ggtgcctttg attacctgtt tcgcaccgcg ccgcccgaac
    19141 cgatgcagat tctgtccagt ctgggactgg tgctgctgat gttccagatc gggatggaat
    19201 tcgaattcgc tcacctgacc gagcgggcca accgccgtgc ggtcctgagt gtggccggca
    19261 tctgtctggc catccctttc atcctgggcg tcggattcgg ctatgtcgtc gcacccgtgc
    19321 tgtcaccgag cgccaaccgc ctgcactcag cgctattcgt ggcaacggcc ttctcgatca
    19381 ccgcgttgcc ggtactcggg cgaatgctga tcgaactgga cgtcacccat acggtgctgg
    19441 ccgtgatcgc cgtcagtgcg gccgccatca acgatgtggt cggctggctg ctgctggcac
    19501 tgatcaccgc gatcaccgcc ggggacttca atggtggcgg ctatggcctg agagtggcgt
    19561 tggtcgccgt ctacgcactg atctgcctga ttgcggtccg tccgctggtc aaggccggcg
    19621 tgcggcgggc gaatccaggc gagcgcgggt tgtccgccaa tctgatcggc gcactgctgg
    19681 cgctggtatt cctgtcggcg atcgcgacct accaacttgg catcttcgcg gtcttcggca
    19741 gcttcatgat gggtgtgatc ctgttcgacg aacgcgaact ggtgcaagcc tggcgcgaca
    19801 ccaccggccg gttcgtgaac gtgttcttcc tgccgatctt ctttacctac acgggccttc
    19861 ggacttcgat cgggagcctg agcagcgctg ccgattgggc ctggtgtgcc ctcaccctgg
    19921 ggctggcctg tgccggaaag ttcggcgggg cgtatctggc cgcaaggctc gccgggcgta
    19981 cccacgccga atcgacctcg ctcggctaca tgatgaatac ccgcggactg atggagctgg
    20041 tggttctgaa cgtgggctat gacctggggg tgatctcccc gcgcatgttc acgatcctgg
    20101 tcatcatggc cgtgctcagc accgcgatta cgatcccggc gctgcgccgc tacctgccgc
    20161 gcgccggaat cgcactggcg gcgtgaagcg cagcggaccg gctgtcaccg ggcaccggta
    20221 tactcaaggc gcatgacacg gcagcgtacc cattcattct ccacagcgtt gctgctgacg
    20281 gcgctgacgc tgctccagcc ggtggccgcc ttggccgacc acaagcctcc caagccggga
    20341 ctgctgctcg gttcgcaccc ctcaaagcag gccaaggccg ctcgcctggc ggtgctgaag
    20401 caacgaacga gcgccgtgcc gctcaaaccc ctccactcgc ccaaggtcac taactcgttg
    20461 aatcatcgtg cgggcaagac catgaccgtt cctcagaggc cgcccaagtg attgatttat
    20521 atggatgtca cggctgtctg tgaattgcga cagggcgccg ggtacgacaa aaggttaaaa
    20581 tgccacccat cggaagctgc tgaatgggaa cctcaaaatg aatcacagac gactgcgact
    20641 gccggcagcc atggcggtaa tggccttggc tgcgccagtc ctcgcagcag gttttggtga
    20701 taatccgggc gagaacccca aggccgatgt ccaggcggca gccgcgaaca tccaacgcga
    20761 ggcccacgcc ttcggcacct ccgtgcggga aaactcccag cagttgcacg atcaggtggc
    20821 gcacagcgtg catcagtttc gccatcagtt cacgatccag tggtaccgga ccggcgactc
    20881 gattcaccgc tggtggaaca acactcggga cagcttctcc aggagctgac ctagcggcgc
    20941 gcgttcagcg tgctgagcat tccgcgcgag cccgaaaccc cggtcgtcgc atccgccagc
    21001 gtggtggctg gcagcagcgt gcgcggattc accgccacat cgccctgacg cagctcgaag
    21061 tgcacatgcg ggccggtgga atgaccggtg ctgcctatca gcgcaatcag cataccctgg
    21121 ccgacatgat ccccctgttt cacccggagc tggctgttgt gcgcgtacag ggagctgagg
    21181 cgctgcccgt gctgaataat gaccacgttg ccgtagccgc gcagcccgtc gccggcgtag
    21241 atgacatcgc cctcggccac ggcatacacc ggctcgccca catccgctgc gatatcgatg
    21301 cccttgtgaa gcttgcccca gcgggcaccg tagtctgagc tgatcactcc ggcctcgacc
    21361 ggccagtgca tgccaccctc ggcgctcatc ggtgccgagg ccagcatcgg cgctgacccg
    21421 gaggactgag gcggccgggc gggtcccgcc ttcggcgatt caggcgtcgg cgcgttggca
    21481 gaggatcctt tgcgagccgc atgcagcacc ttcagcacct cggcttcact gtgttcagtc
    21541 gcgtcttcga aattcttcag cgtgtcgtgt tcggccagat acttctgctg gcgctcatag
    21601 accgcctggg cgtggacctt ggctacctgc gccagttgcg tcgccttgtc tttgacagaa
    21661 tccacggtac tgctgacgct cgcgcatccg ctcagtgcca gccagatgct cgtgcctgcg
    21721 atgcgaagag tcgtgagacg catgttgaga tcctccctgc cctgtggcga ggatcttccc
    21781 agccagcgca tcaccgacat acccccacaa aggggggtcg ccgccggcga gcgatcgacc
    21841 ctacgatcgc tccatcaggg aggttccact catgcaacgt caaatcgata tcgccaagcg
    21901 cctcgatgtt ccctcgctca tcgccttgag tttggtcgcc atcggctggt tcggcatgaa
    21961 cacgctggtg atcagtgtcg ggcggctggt tcaagcgacc cgcttctacg aggtgggtgt
    22021 cgtcatactg catccggcag cgttgttcct gggagtgggc agcgggcata cgttcgaact
    22081 gaccgcattc gcgctgctcg cgctgttgac gctgttcgcc gcgatggcag caccggtttt
    22141 gtttgcgcgg cgttgcgcct ggctcgcagg ttgggcgcca ctcgtgttga tgctggtgtg
    22201 cgccgcgttg ctctacggtg acggctcgag cccgccgcca ccggccgccg atccgggtct
    22261 gcgaggcgat ctgatgcgct ttgccaatca gctgctgcag cacgcgcaag acacgttggt
    22321 cagtcacatc agcgtgggtg ccggcggcat cctctcggcg ctggccagcc tgatcgtggc
    22381 ggtccgctcc acccggaggt tccgggtgga gtcgcctgaa gtggcattcg cggctgcacc
    22441 ctcctacggc tggacgtgaa cctgagccct agacggtgaa ggtaccgaac gatccgctgc
    22501 ggcccgcagg tagggtcttg atccggccaa gctcttgctc gagtttgtcg agcgtctgac
    22561 tccatccgag cttggcgccg ccaatcatcg ccgcggccat cgacgcatcg atcagcgtgt
    22621 aggtttgcgt gacctcgatg cgtgtcgcac cgccgggcag gtccacgaag ttgacgaccg
    22681 tgtaggcgcg aaacaagggc tggttgtcat ccccgaccgc gtgcatgtcg atcaccagtc
    22741 ggctgttggg aacaatctcc acgaacttgc cgcgtgacca gtgtcgctgc ccttgcggcg
    22801 actgcatgca cacattgaat gcgccgccga cacgcaactc gatctcggcc tccggcacgc
    22861 tgtagggctc gggactgaac cactgcttca cgtgttcggc gcggctccac gcgcggaaca
    22921 ccagctcgcg cgtagcggca aaagtgcgag cgacgacgag ttcatttgtc atggggatcc
    22981 tcttccattt gtttcagggt cttgaggtat tcgcccagcc ggtcgaaatg cgcttcccac
    23041 tcggcgcgcc gggcattgat ccattgctcg gccagaccca agccctgggg ctcgatgcgg
    23101 caagtgcgta cccgcccccg ttttgcggtc cggaccagac cgcagtgctc aagcaccgcg
    23161 aggtgttgca tcaccgcggg caacgacatc gccagcggct tggccagctc gctcaccgaa
    23221 atcgcgcgtc ggctgagttg cgccaggatg gaacgtcggg tcgagtcgga cagcgcctga
    23281 aaggtacggt cgagtgcaac agatttgtta agcatgttct taagtatagc ggcattcaat
    23341 acttaagcaa agtatttagt atctgatcgt cagtgggtcg cgctcgcctg ggtggtagcg
    23401 agcgcggcat cggccgggcg ctcgcccttc cagcgtcggt gaatccaggt ccagtccgcg
    23461 ggactggctc gaatctgcgc ttcaatcagt gcggcatagc gcgcggtgaa gatcggcgga
    23521 tccaggcgct cgttcgcgcc gcagatcggg atgacctcga gtgtgtagtg cccgcgccgc
    23581 tcgcgtcgca tcgcgatgaa aaacgcggcg taaccggtca tgcgggcaat ttcggctgga
    23641 ccgggataga aagcggtgtc gcgtccgaga aagctgaccc agtgccggcc ctgactggag
    23701 gttggcatct gatcggccag caaggcgacc gcatgtacct gcacctgccg gcgacgcgag
    23761 atcacttcgc gcaccagtcg tttggcggcg atcaggttgg cgccatagcg aactcgcagt
    23821 ttacgcagtt gcgcgtcgga tttctggccg tgcagcggtt tgtaggccgc gtccaccggc
    23881 acattcatct gcaacaccgt gccatgaaac gcccattccc agttgcactg atgcgccgcc
    23941 accagaatca ccgagcgacc ggcagcggtc tcagcgcgca cttgctcgaa tccgatcgcg
    24001 ctgaggtgat cgcgcatctg ctgcgccgac atgaccccga tcttgaggaa ctccactgcg
    24061 atctggccga gctgacgata gtaggcgcgc agccacgtgt tgatctgctt ggcggaagct
    24121 tgcggaaagc aacgcgaaag attgtcacgg gcgatcgaga cgcgaaaacg aaacacgtag
    24181 cgcgccagga aggcgagcac cgcggccttg gcatagagca cgccccacgg caacgaggcg
    24241 aacactcgga gccacagcga gtcattggca ggtgcctgat tcatttatag tgtcgtgatg
    24301 ctctggg
//
LOCUS       DATMRM010000137        14847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3169266, whole genome shotgun sequence.
ACCESSION   DATMRM010000137 DATMRM010000000
VERSION     DATMRM010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14847
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3169266"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..105)
                     /locus_tag="VNZ06_04040"
     tRNA            complement(30..105)
                     /locus_tag="VNZ06_04040"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70..72),aa:Arg,seq:cct)
     gene            complement(116..1027)
                     /locus_tag="VNZ06_04045"
     CDS             complement(116..1027)
                     /locus_tag="VNZ06_04045"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWW19954.1"
                     /translation="MKFITFRYQDQVLPGLVTADRVLNLAAAAKSAGEKLDFSSLLAI
                     IRGGEPALQACQRLESRAGDRSEELIPLSQVQKLAPIPVLTRNAFCVGRNYVDHIKEG
                     VNARAMKWDEKLPEVPQFFTKSTHTVIGPGAEVRLDTKLTQRLDYEVELAVVIGKAGR
                     DIPVARAYDFVFGYTIANDVTARDLQRRHEQWFKGKSLDTTLPLGPCIVTRDAIGDPR
                     GLELSMSINGQERQRARVEQMIFDIPTIISQLSAGMTLEPGDIIATGTPSGVGFAMTP
                     PQFLHDGDEMLATIDRIGELRNRVVEV"
     gene            complement(1051..2298)
                     /locus_tag="VNZ06_04050"
     CDS             complement(1051..2298)
                     /locus_tag="VNZ06_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWW19955.1"
                     /translation="MSKSAALAGIRVLDLSRILAGPWCTMTLADLGAEVWKIENPVGG
                     DDTRHWMPPEKNGVSTYYLSANRNKRSVAIDLNQEAGRDLIRALAAKADVLVENFRPA
                     SLKRWNLDYPAIRSINPKIIYCSISGYGRGGEFEERPGYDFVLQAECGFMAITGEPEG
                     TPMRLGVAFIDLATGMNAVQGILAALFARERTGVGQWIDISLLDSGLHQLANVGSGVL
                     NSGREPKRHGNAHPSIVPYEVFECGDGVNVALAVGNDEQYRRLCVSVFETPELWDNEK
                     YRSNHGRTTHRAMLIPLLQAQFKRFASPELMGKIREHGISVGEVRLPGAALQSPEAVA
                     RQCVVTAPSESLGTVRMVASPLRLSETPVVAPIAAPALGQHTREVLSGVLGLSEAKVA
                     QLSAQGIVALTEVNASVPEIDPQ"
     gene            2373..2852
                     /locus_tag="VNZ06_04055"
     CDS             2373..2852
                     /locus_tag="VNZ06_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWW19956.1"
                     /translation="MKYFNGIIGSVVLAFAVTLVLAADSTAQFIRLTPEQVPFRNPSG
                     FEQAVLFGDPMKPGLYVVRVRFPPGIHSNPHSHSQDRHVTVIKGVWYMGTGETADIAK
                     ATPLKSGSYAFHPAHGVHWDGAGAEETIVQIVGLGPVETVQTDPRQPPVGSWTPPKN"
     gene            complement(2853..5153)
                     /locus_tag="VNZ06_04060"
     CDS             complement(2853..5153)
                     /locus_tag="VNZ06_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWW19957.1"
                     /translation="MFSLRTRIAVTFLVLLAAVLTAALSVVSAANRSNAEREVRRQLS
                     VGSSVFSRLLESDRRQLKTAAKAVADDYAFREAVATGDTDTSSSALENGAARIGAAMA
                     VLSSMEGKVIASYGKHAIASAHLPQARDGDGSIIVDDGRIYQLVTVAVRSPLPVALVT
                     MGFELDAHAAQELADVTGLAVTFSYRAGGDWKAGISTAPAARAADVIAQEIEMSKVGG
                     SEVRATLSRSLADARAPFERLSNVLIGIAIVSVAVAAAAILWLSRGITQPLRELTNAV
                     DKIRGGSYDVSVRVQRRDELGVLAEGLQLMQSAVLSRDRSIRQLAYEDPLTGLMNRTA
                     FVAAVGEGLNCGESALGVAVINLHRFRRINEHLGYSVGDAVLTKIGVGLASVPSVKGA
                     VARLAADQFAAFTRLPENVSLQLWGASLLEALSEPVLVESQPIDLTASVGLAVAGDVR
                     MSADELMVCADLALERARRDKLALAVYDPALKPMSRDQLSLLGELRRAIEQDELRLYF
                     QPKIELGSQRVAGAEVLLRWQHPTRGLLSPASFIPFAEQTGFIRRLTRWTLDRAIAQG
                     AAWFNAGKPLSLAVNISADDVGDARLDSRVATLISRHQLPPSLLTLEVTESGFIEDPN
                     RALRILDALAALGVLLSIDDFGTGYSSLSHLARMPVHEVKIDRSFVTGLESNVEFAAI
                     VRSAIEMGHSLGLKVVAEGIETDTAALKLRSLGCDIVQGYLYAQPMCLADLEKWLQSK
                     ARVRVNAQPVNLPVGDIAETATRHIF"
     gene            complement(5155..5784)
                     /locus_tag="VNZ06_04065"
     CDS             complement(5155..5784)
                     /locus_tag="VNZ06_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HWW19958.1"
                     /translation="MNLSSTGFHALALTAMWCVGRVYAGTVTVEISSSGTVSAQDTIV
                     VFDPLDTTVAPARTQATIDQVDKQFVPKISVLRTGTTVIFTNSDHIRHQVYSFSPSKT
                     FTLKLYAGSPEAPVVFDKPGLVVLGCNIHDNMLAFVGIVDSPYFGKASKSGSVALNLP
                     AGRYRLRVWHPSLRQPMPSREIAVSASAMTIPLTLDLTPNAPGVAPWPE"
     gene            complement(5781..6227)
                     /locus_tag="VNZ06_04070"
     CDS             complement(5781..6227)
                     /locus_tag="VNZ06_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HWW19959.1"
                     /translation="MTAVTFRVLALSLAAFAGIAAGRAHVSLYDLLGGQDGVAHIANT
                     LIDRVATDPSLKRSFEGSNLRRIKTLLAEQICDLSGGPCHYSGDPMKEAHAGHHISEA
                     EFYGMVAALRDVLKADHVGQGASNELLALLAPMKRDIVEPASGQAK"
     gene            6360..7223
                     /locus_tag="VNZ06_04075"
     CDS             6360..7223
                     /locus_tag="VNZ06_04075"
                     /inference="COORDINATES: protein motif:HMM:NF022677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HWW19960.1"
                     /translation="MIARARRGRILASMCLLLSAAPTHGGDRLEWTGATTEIEGSAGG
                     GLVPWALIGGLETNDQVGATAFATYNATPDFSLRTAGISLDVHDRWEVSFVQQRLDAA
                     STPRAAVLGQDILGLKLRILGDAVFSPDRVLPQIAVGAQFKYTLNFDGIPKAVGAARA
                     DDVDLYLTATKLYFAAIADRDVLVDLTVRRTRANQFGLLGFGADRHGYRLVPEASIAI
                     FLTQSLLLGAEYRAKPDNLSGFREDSAQDLFLAWAPWKNLELTAGVSDLGRIAGKSDQ
                     RGLYLSVWVGI"
     gene            7220..8164
                     /locus_tag="VNZ06_04080"
     CDS             7220..8164
                     /locus_tag="VNZ06_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWW19961.1"
                     /translation="MKKASVDASQREQYAAGMPDEDDEKTRVTGLEEMQVQPAGDDCL
                     VVIHTAVQTELGRRYLLNEGVITIGRGAANQIVVSSDAVSRQHAQLERRGADFLVSDL
                     NSTNGTFINNERQRTRNSRLNRGDQIRVGDTVFKYLSGSDIEAQYHAVIGHMAVTDGL
                     TNLANRKHLDTLLAEEVQRAHRHNRELSVLMLDIDHFKTINDAHGHLAGDRVIAGLGH
                     LLRQRLRSDDKLGRYGGEEFCAILPETGLDNATTIAETLRTLVATHPFPVDAKRLTVT
                     VSIGAAALKPQMHSTDLYRAADEMLYRAKNQGRNQVCS"
     gene            complement(8196..8271)
                     /locus_tag="VNZ06_04085"
     tRNA            complement(8196..8271)
                     /locus_tag="VNZ06_04085"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8236..8238),aa:Ala,seq:cgc)
     gene            8408..8872
                     /locus_tag="VNZ06_04090"
     CDS             8408..8872
                     /locus_tag="VNZ06_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWW19962.1"
                     /translation="MLKAGDRAPEFSLPDHNGRDTSLTSLLNAGALILWFFSGLPNPT
                     SMIAARRIGELHADLRRQGLVVAAVSPRTPDRLQRLRDRHRLPFVLLSDTQRAVARMY
                     ERTGILGVWRGTYLISRGRTILGSVANPVGLDKHLDFMREAPELVLGMPLHF"
     gene            8974..9168
                     /locus_tag="VNZ06_04095"
     CDS             8974..9168
                     /locus_tag="VNZ06_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19963.1"
                     /translation="METRTDVQQLLRRHREIRQAMRQPGGVRILDERELYAIREQLKG
                     IPAAMQLLQRDATLSSPSMR"
     gene            9176..10981
                     /gene="treZ"
                     /locus_tag="VNZ06_04100"
     CDS             9176..10981
                     /gene="treZ"
                     /locus_tag="VNZ06_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HWW19964.1"
                     /translation="MSSSPRRSRPFGAELEDDGRVRFRLWAPGHERIELELQITGAQG
                     ARLLLPMQREPDGWHGLISSAARAGSLYKFRLPDGTVVPDPASRYQPSDVHGASQVID
                     PNAYRWVDGDWRGRPWHEAVVYELHLGCFTSAGTYLAACERLDHLASLGVTAVEVMPV
                     ADFPGRRNWGYDGVLPYAPDASYGRPEHFKAFIDTAHQKGLMVILDVVYNHFGPEGNY
                     LSLYCPQFFNERHQTPWGAAIDFDGHDSRIVREFIIQNALYWLEEFHLDGLRLDAVHA
                     IRDDSPTHLLTELAQRVRAAELPRAVHLILENEHNEAIRLSASLYDAQWNDDVHHALH
                     VAASGEREGYYADYVGDTEKLGRALAQGFAYQGEIMPYRGSARGEPSAKLPPRRFVAF
                     IQNHDQVGNRALGERLHQIAPSAAVGAVTAVYLLLPQIPMLFMGEEWHAGQPFQFFCD
                     FGAELAQAVRDGRRQEFSRFAAFQSEQARDRIPDPQSEQTFLDSKLDWTALEQPSHAS
                     ALRWYQELLAARRRHVVPLISAIGHGGRYTVIDHGAVMVQWQSPVAELTLLANLSARR
                     VTGFPEVPGHLLWEQGQIEASGTYPPWCVRWMLRT"
     gene            10951..13773
                     /gene="treY"
                     /locus_tag="VNZ06_04105"
     CDS             10951..13773
                     /gene="treY"
                     /locus_tag="VNZ06_04105"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HWW19965.1"
                     /translation="MVRPLDAAYVIPRATYRLQLHGGFGFSAATDCVPYLARLGVSHA
                     YLSPYLKARAGSAHGYDIVDHSQLNPELGDEEAFQRMSQALKAHSLGHILDFVPNHMG
                     VGGADNPLWLDVLEWGPDALHAGWFDVDWDPDRRYLHNRILVPFLGDQYGIELERGVL
                     RLRFDESDGSFAVWAYDTHKLPIWPPHYARILTDGTAQLERLADAFNWLPNWHQQMPQ
                     RAAELKTRLAALVRDHADAREAVQRAVQRFEGQPDQSGSWQSLDELIAEQRWRIAHFR
                     VAADDINYRRFFNINDLAGLRMELPEVFDHVHQRVLRWVKDGTLDGLRIDHIDGLYDP
                     KGYLRRLQRRLSTRHAEHGFYLVVEKILAAHEPLREDWPIDGTTGYDFLNQVLALLID
                     SSAYADFDRCYRQFVGEPQRFADIVHQSKRHIMENEMASELNVLARDMGRLARQNPRT
                     ADFTRNLLRRAIKELIACFPVYRTYIDDAGELDSADQRDLSWAFAQARRNESELDPSV
                     FNFLEQVLTGALLQRSRTGFSHQSLLRCAMRLQQYSGPVMAKGVEDTAFYRYHRFIAL
                     NEVGGDPERFGATLSSFHKSNQARAHRWPHAMLATATHDTKRGEDARARLAALSEFAE
                     EWIQQLPVWSRILRGPLIAEDAPLKPDRNDEYLLLQLLLGTWPCELMTSEVRDAQLSK
                     DYAQRIRGAMVKSMRESRLHTGWAFPNAEYEEATLGMIDAALTGARSSAFLSSFLPFV
                     RKVADCGVDKSLIQTVVKLTSPGVPDLYNGSELWDLSMVDPDNRRPVDYALRTRLLRQ
                     IDERLAADRALGMSELRRNWCDAGIKLATIATLLRHRRDHERLYAEGDYQGLPASGAR
                     ADDLCAYTRHRNGDKLLVAAVRRAHRADGSKQWEDTVLPVPEALRREQWHELLTGRLV
                     ALREDTVGPADLFAELPVVVLASNGH"
     gene            complement(13770..14057)
                     /locus_tag="VNZ06_04110"
     CDS             complement(13770..14057)
                     /locus_tag="VNZ06_04110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWW19966.1"
                     /translation="MGDQVLDNAAESRYEMKLDGGTAFIDYTAAGRVRTLTHAEVPAA
                     LRGGGVAARLTSGALDLVRSQRIKVIPRCPYVRTFIERHPEYQDLLASPPG"
     gene            complement(14064..>14847)
                     /locus_tag="VNZ06_04115"
     CDS             complement(14064..>14847)
                     /locus_tag="VNZ06_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWW19967.1"
                     /translation="QPAELPPKISGRIAFENVSFSYPSRSDTRALREYSLEVRPGETV
                     AFVGPSGAGKSTTFQLLLRFYDPQAGCISIDGIDIRTWRPEELRSKIGFVPQDTVLFG
                     ATARENIDYGRPGSTDEQIVAAASAAAADEFLRELPEGYNTYLGERGVRLSGGQRQRV
                     AIARAILKDPPILLLDEATSSLDAESERLVQAALNVLMRGRTTLVIAHRLSTVLRADR
                     IVVLQAGRIVAIGKHAELMHGSPLYAKLAALQFNAAEEALEV"
BASE COUNT         2784 a         4716 c         4617 g         2730 t
ORIGIN      
        1 aaatgaccgc tcgtaaggcc cttgaaaact ggcgcccccg gccggagttg aaccgacgac
       61 ctaccgctta ggaggcggtc gctctatcca ctgagctacg ggggcgcacc ggaaattaaa
      121 cctcgaccac gcgatttcgc agctcgccga tgcggtcgat ggtggcgagc atctcatcac
      181 catcatgcag aaactgcggt ggtgtcatcg cgaagccaac acccgacggc gtgccagtcg
      241 cgatgatatc gccgggctcc agagtcatgc ccgccgacag ttgcgagatg atcgtcggga
      301 tatcgaagat catctgttcg acgcgagcgc gctgtcgctc ctggccgttg atcgacatcg
      361 agagttcgag ccctctcgga tcgccaatcg cgtcgcgcgt cacgatgcac ggccccagcg
      421 gcagcgtcgt atcgagcgac ttgcccttga accactgttc gtgtcggcgc tgcaggtcac
      481 gagcggtcac gtcgtttgcg atggtataac cgaatacaaa atcgtaggca cgcgcgaccg
      541 gaatatcgcg ccccgccttg ccgatcacta cggccagctc cacttcatag tcgagccgct
      601 gtgtcagctt ggtgtccagc cgcacttcag cgcccggacc gatgaccgta tgcgtcgatt
      661 tggtgaagaa ctgtggcacc tccggcagct tctcatccca cttcatcgcc ctcgcgttga
      721 caccctcctt gatatggtcc acgtagttgc gtccgacgca gaaggcgttg cgtgtgagca
      781 ccggtatcgg cgccagtttc tgcacctgcg acagagggat gagttcctcg ctgcgatcgc
      841 ccgcccggga ttccagccgc tggcaggcct gcagcgcagg ctcgccgccg cgaatgatcg
      901 cgagcaggga cgagaaatcg agcttctcgc cggctgactt ggccgccgca gccagattca
      961 gaacgcggtc cgcggtcacg aggccaggca gtacctgatc ctgatatcga aacgtgatga
     1021 atttcattcg gtgaatttta ccgactttgg ctactgcggg tcgatttccg gcaccgaagc
     1081 gtttacttcg gtcaacgcga caatcccctg cgcactcaat tgagccactt tagcctccga
     1141 caggcccaat acgccggaga gcacctcccg ggtatgctgc ccgagtgccg gcgcggcaat
     1201 gggggcgacg accggtgttt cagacagacg cagtgggctg gcaaccatgc gcaccgtacc
     1261 cagcgactcg ctgggagccg tgacgacgca ctgacgcgcc accgcttcag gtgattgcaa
     1321 cgccgcgccc ggcagacgca cttcgcccac cgatatcccg tgttcgcgga tcttccccat
     1381 caactccggc gacgcgaagc gtttgaactg cgcctgcagc aggggaatca acatggcgcg
     1441 atgggtcgtg cggccatggt tgctgcgata cttttcattg tcccagagtt cgggagtttc
     1501 aaatacgctg acgcacagcc gccggtactg ctcgtcgttg ccgaccgcga gcgccacatt
     1561 gacgccatcc ccgcactcaa agacctcata cggcacgatg ctcgggtgcg cattgccgtg
     1621 gcgcttcggt tcacggccgc tgttgagcac gccggagccc acattggcca gttgatgcag
     1681 cccgctgtcg agcaatgaaa tgtctatcca ctgtccgacg ccggtgcgct cgcgcgcaaa
     1741 caacgcggcc agaatcccct gtactgcgtt catgccggtc gccagatcaa tgaacgcaac
     1801 acccaggcgc atcggcgtac cctcgggctc gccggtaatg gccatgaaac cgcattcggc
     1861 ctgcagaacg aaatcgtatc cgggccgctc ctcgaattcg ccgccccggc catagccgga
     1921 aatcgagcaa tagatgatct tcggattgat cgagcggatc gccgggtagt ccagattcca
     1981 gcgcttgagg cttgccggcc ggaaattttc gaccagcacg tccgctttgg cggccagcgc
     2041 acggatcaga tcgcgccctg cttcctggtt caaatcgatc gcgacactgc gcttgttacg
     2101 attggcgctc aggtagtagg tggagacgcc gttcttctcg ggtggcatcc agtgacgcgt
     2161 gtcatccccg ccaacgggat tttcgatttt ccacacttcg gcgccgagat ccgccagcgt
     2221 catcgtgcac cacggcccgg ccagaatgcg gctgagatcg agcacacgga ttccggcgag
     2281 agcagcggac tttgacatac cgttcctgac tgggtgctga gcgtcgtact attgcatagt
     2341 actgaacagc tatcgtaagc ggggggaggt gaatgaaata cttcaatggc atcatcggat
     2401 ctgtggttct ggcgttcgcc gtgacgttgg tactggccgc tgacagcact gcgcagttca
     2461 ttcgcctgac gcccgagcag gtgcccttta ggaatccgtc agggttcgaa caggcggtgc
     2521 tgttcggtga cccgatgaaa cctgggctct acgtggtacg ggtgcggttt ccgcccggca
     2581 tccactcgaa tccgcactct cattcccagg accggcacgt cacggtcatc aagggcgtgt
     2641 ggtacatggg caccggagag acggcggata tcgccaaagc gacgccactg aagagcggct
     2701 cgtatgcgtt tcatccggcg cacggcgtgc attgggatgg cgcgggcgcg gaagagacca
     2761 tcgtccagat cgtggggcta ggtccggtcg agacggtgca gacggatccc aggcagcctc
     2821 cggtcggctc ctggacgcca ccgaagaact gactagaaaa tgtgtctcgt agcggtctcg
     2881 gcaatgtcgc ccaccggcag attgaccggc tgcgcgttga cgcgaacacg cgccttgctc
     2941 tgcagccatt tttccagatc cgcgaggcac atcggctgcg cgtatagata gccttgcacg
     3001 atgtcacaac cgaggctgcg aagttttagc gcggccgtgt cggtctcgat cccttcggcg
     3061 accaccttca aaccgagact gtgtcccatt tcaatcgctg agcgcacaat cgccgcgaat
     3121 tccacattcg actccagacc ggtgacgaaa ctgcgatcaa tcttgacctc gtgcaccggc
     3181 atgcgagcca gatgactgag tgacgaatac ccggtgccaa aatcgtcgat tgacagcaac
     3241 acgcccagcg ccgccagcgc atccagaatg cgcagcgcgc ggttgggatc ttcgatgaat
     3301 ccgctctccg tgacttccaa tgtcagcagc gaaggcggca gctgatgccg ggaaatcagc
     3361 gttgctaccc gcgaatcgag ccgcgcgtca ccgacgtcgt ctgcgctgat attgaccgcc
     3421 aggctcagcg gtttgccggc gttgaaccac gccgcacctt gcgcgatcgc ccgatcgagc
     3481 gtccagcggg tcagacgccg tatgaatccg gtctgttcgg caaacgggat gaatgaggcg
     3541 ggtgacagca gcccacgggt aggatgctgc catcgcaaca gtacttcagc acccgcgacc
     3601 cgctggctgc ccagctcgat cttcggctga aagtacaatc gcaactcatc ctgttcgatt
     3661 gcccggcgaa gttcgccgag caaggagagc tgatcgcgcg acatcggctt gagcgccggg
     3721 tcgtacactg ccaacgcgag cttgtcacgt cgcgcgcgct ccaacgccag gtccgcgcac
     3781 accatcagtt catccgccga catcctgaca tcgcccgcca cggcgagccc gaccgatgcg
     3841 gtcagatcga tcggttgcga ctcgaccagc accggttcgc tcaaggcttc cagcagtgac
     3901 gcgccccaga gctgcaagct cacgttttcc ggcaaacgtg tgaaggcggc aaactgatcg
     3961 gcggccagtc gcgcgaccgc gcctttgacc gacggcaccg aagcgagccc gactccgatt
     4021 ttggtcagca cagcatcacc gaccgagtag ccgagatgtt cattgatgcg acgaaagcga
     4081 tgcagattga taaccgcgac gccgagcgca ctctcaccgc agttgagccc ctccccgacc
     4141 gccgcaacga atgccgtgcg attcatcagg ccggtcagcg gatcttcata ggcaagctgc
     4201 cgaatggagc gatcgcgtga caacaccgcc gattgcatca actgcaatcc ttctgccaac
     4261 acgccgagct catcgcgccg ctgtacgcga accgatacgt cgtacgaccc accccgaatc
     4321 ttgtcgaccg cattggtgag ctcgcgcagc ggctgggtga tgccgcggga cagccacagg
     4381 atggccgcag ccgctactgc cacactgacg atcgcgattc cgatcagcac gttgctcaga
     4441 cgttcaaacg gtgcgcgcgc atcagccagc gatcgcgaca gcgtcgcgcg aacctcgctg
     4501 ccgcctactt tcgacatctc gatctcctgg gcgatcacgt ccgcggcacg cgcagccggt
     4561 gccgtgctga tgcctgcttt ccagtcccct ccggcgcgat aggagaacgt taccgccaac
     4621 ccggtgacat cggcgagttc ctgcgcggcg tgtgcgtcca gctcgaagcc catggtgacc
     4681 agcgccacgg gaagcggact gcgcaccgcg accgtgacca actgatagat gcggccatcg
     4741 tcgacgatga tgctaccgtc accgtccctg gcttgcggca aatgggcgct ggcgatcgca
     4801 tgcttcccat aggacgcgat caccttgccc tccatgctcg acagcactgc catcgcagcg
     4861 ccgatgcggg ccgcgccgtt ttccagtgcg gaagaggacg tgtccgtatc gccggtggcc
     4921 accgcttccc gaaacgcgta gtcgtcagcc acggctttgg cggcggtctt gagctgacgc
     4981 cgatcgctct ccagcaatcg cgagaacacc gaggatccca ccgacagctg tcggcgcacc
     5041 tcacgttcag cattgctgcg attggcggcg gagacgacgc tcaatgcggc agtcagcacc
     5101 gcagccagca gtaccaggaa ggtgaccgcg atgcgcgtac gcagcgagaa cattttactc
     5161 gggccagggc gcgacaccgg gagcgttcgg cgtgagatcg agggtcagtg gaatggtcat
     5221 ggccgaggcg gagacggcga tctcccggga cggcatgggt tggcgcagac tcgggtgcca
     5281 gacacgcaga cggtagcggc cggcgggcag gttcagcgcg acgcttcctg acttggacgc
     5341 cttgccgaaa tacggagagt ccacgatgcc gacgaaggcc agcatgttgt cgtgaatatt
     5401 gcagccgagc accaccagac ccggcttgtc gaacaccaca ggcgcctcag gtgagccggc
     5461 gtacagcttc aatgtgaagg tcttggacgg ggagaatgaa tagacttgat gacggatgtg
     5521 atcgctgttg gtgaagataa ccgtggtacc ggttcgaagt acgcttatct tcggcacgaa
     5581 ctgcttgtct acctgatcga tcgtggcttg ggtgcgcgcc ggcgccaccg tggtatcgag
     5641 cggatcgaac accacgatgg tgtcctgcgc tgacacggta ccgctgctgc tgatttcaac
     5701 ggtcacagtg ccggcataga ctctaccgac gcaccacatc gcagtgagcg cgagagcgtg
     5761 aaatccggtg ctcgacaggt tcatttggct tgtccagacg ccggctcgac gatgtctcgc
     5821 ttcatgggag ccagcagcgc cagcagctcg ttgctcgcac cttggcccac gtggtcggcc
     5881 ttgagcacgt cacgcagcgc cgcgaccatg ccgtagaact cagcttccga aatgtgatgg
     5941 ccggcgtgcg cttctttcat cgggtcgccc gaatagtggc aagggcctcc gctcagatcg
     6001 cagatctgct cggccagcag tgtcttgatc cggcgcaggt tcgaaccttc gaagctgcgc
     6061 ttgagagatg ggtctgtcgc gacgcgatcg atcagcgtat tggcaatgtg agcgacgcca
     6121 tcctggccgc ccagcaggtc gtagagactg acgtgcgcgc gacctgccgc aatgccggcg
     6181 aatgcagcca gcgacaaagc cagcacccga aatgtgacgg ctgtcataca tggccccgtg
     6241 aaaacgtcag gttaaagcag cggcgtcgcc gcagctgtgc ggttggacac aatatttcgg
     6301 ccttggtgcg cctacccttg ctgcgctttg tcagatgacg aattggatcc aacgaatacg
     6361 tgattgcacg cgcgcgccgg ggccggattt tggcctcgat gtgtctgttg ttgtcggccg
     6421 ctccgacgca cggcggcgac cggctggaat ggaccggagc gaccaccgag attgagggca
     6481 gtgcgggtgg cgggctggtc ccttgggcgc tgatcggtgg gctggaaacc aacgatcagg
     6541 tcggtgccac cgcttttgcc acttacaatg cgacccccga cttctcactg cgcactgcgg
     6601 gcatcagcct cgacgtgcac gaccggtggg aggtttcatt tgtccaacag cgactcgatg
     6661 ccgcctcgac gccgcgcgcc gccgttctcg ggcaggacat ccttggcctc aaactgcgga
     6721 ttctcgggga cgcggtgttc tcccctgacc gggtgctacc gcaaatcgcc gtgggcgcgc
     6781 agttcaagta caccctgaat tttgatggca tcccgaaggc cgtgggtgcg gccagggccg
     6841 atgatgttga tctgtatctg acggcgacca agctatattt cgctgcgatc gccgaccgcg
     6901 acgtgctggt ggaccttacc gtgcgacgca cgcgcgccaa ccaattcgga ctgctgggct
     6961 tcggcgcgga ccgccacggc taccgccttg taccggaggc ctccatcgca atattcctga
     7021 cgcagagcct gttgctgggt gccgagtacc gtgccaaacc cgacaatctg agcgggtttc
     7081 gtgaggacag cgcgcaagac ctttttctcg cctgggctcc atggaagaat ctcgaactca
     7141 cagccggtgt tagcgatctc ggccgaattg cgggaaaatc cgatcagcgt ggtctttatt
     7201 tgtcggtgtg ggtgggaatt tgaagaaggc ctcagtagac gcatcgcagc gtgagcagta
     7261 cgctgccggc atgccggacg aggatgacga gaagacccgg gttaccgggt tggaggagat
     7321 gcaggtccaa cccgccggtg atgactgtct ggtcgtgatc cacacggctg tacagaccga
     7381 actcggtcgt cgctaccttc tcaacgaggg cgtcattacc attggacgag gcgccgccaa
     7441 tcaaatcgtc gtcagcagtg acgcggtatc acgccagcac gcgcagctcg aacgccgcgg
     7501 ggcagacttc ctggtgagcg atctgaactc caccaacggc accttcatca acaacgagcg
     7561 ccagcggacc cgcaattcgc gtctcaatcg cggcgatcag atccgagtgg gcgacaccgt
     7621 cttcaagtac ctgtccggtt cggacatcga agcccagtat cacgcggtga tcgggcatat
     7681 ggccgtgacc gatggactga ctaacctcgc caatcgcaag cacttggaca ccctgctggc
     7741 ggaggaagtc cagcgcgcgc atcgccacaa tcgcgagcta tcggtgttga tgctcgacat
     7801 tgaccacttc aagaccatca acgatgcgca cggtcatctc gcgggcgatc gcgtgattgc
     7861 gggcctggga catctgctgc ggcagaggct tcgctccgac gacaaactcg gtcgatacgg
     7921 cggcgaagag ttctgcgcga tccttcccga gaccgggctg gacaatgcga cgacgattgc
     7981 cgaaacgctg cgcaccttgg tggcgaccca cccatttccc gtcgatgcca aacgcctgac
     8041 agtcacagtc tcgatcggcg cggccgcgct caagccgcag atgcacagta ccgatctgta
     8101 tcgcgccgcc gacgagatgc tctaccgtgc caagaaccag gggcgcaacc aggtctgcag
     8161 ttagtcccat cgagcgaccc agcttgactc gcaaatggtg gagcggaagg ggatcgaacc
     8221 ctcgaccttc gcattgcgaa cgcgacgctc tcccagctga gctaccgccc cgtcaggcgc
     8281 gcgattctag catccggggg tcgcgaggca aggcacttgt cggccgattc ccacggcgcc
     8341 ccgcggtaat accaactagg actcagcgca caccctcccg catcctgcgg ccgaggagga
     8401 cgaagtcatg ctcaaggccg gggaccgggc accggagttt tcgctccccg accataatgg
     8461 gcgggacacc tcgctgactt cgttgctgaa cgcgggcgca ttgatcctgt ggttcttttc
     8521 cggtttgccg aaccccacga gcatgatcgc ggcgcgcagg atcggcgagc tgcatgcgga
     8581 cctgcgccgg caggggctgg tcgtggccgc agtgagcccg cgcacaccgg accggcttca
     8641 gcgtctgcgt gaccggcatc gcctgccttt cgtgctgctc tcggacactc agagggcggt
     8701 cgctcggatg tacgagcgca ccggcatact gggggtatgg cgcggtacct acctgatcag
     8761 tcgcggccgc accatcctgg gttcagtggc caatcccgtc ggcctagaca agcatctgga
     8821 tttcatgcgg gaggccccgg agctggtact gggaatgccg ctgcatttct aggatcggac
     8881 ggcgctgaca gcgcattggc cttaagcgct ctggcgccta ctgtgccggt gccgaatact
     8941 tgttgcgtcg cgactccagt gggaacacaa cttatggaaa ccagaaccga cgtacagcag
     9001 cttctgcgtc gtcaccgcga gatacgacag gccatgcggc agcccggggg cgtacggatc
     9061 ctggacgagc gcgagctgta cgcgattcgc gagcagctca agggcattcc ggcagcaatg
     9121 cagctactgc agcgagacgc gacgctctcc tcgccttcaa tgcgctgaca ggcgggtgtc
     9181 ctcttcgccg cggcgctcga ggccgtttgg cgccgagctg gaagatgatg ggcgcgtgcg
     9241 cttcagactc tgggcgcccg ggcatgaacg cattgagctg gaacttcaaa ttaccggcgc
     9301 gcaaggcgcc cgcctgctgc tgccgatgca gcgtgagccc gatggctggc atggcctgat
     9361 cagctcggcg gcacgagccg gctcactcta caagttccgc ctccccgacg gcactgtcgt
     9421 gccagatccg gcttcccgct accagccgtc ggatgtacac ggcgccagcc aagtcatcga
     9481 tcccaacgct tatcgatggg tcgatggcga ttggcgtgga cggccctggc acgaagcggt
     9541 cgtctatgaa ctgcacctcg gatgtttcac ctcggcgggc acctatcttg cggcatgtga
     9601 gcggctggat catctggcga gcctcggagt caccgccgtt gaagtgatgc cggtggccga
     9661 tttccccggc aggcgcaact ggggctacga cggcgtgttg ccgtatgcgc ccgatgcgag
     9721 ctacggcagg cctgagcatt tcaaggcgtt catcgacacc gcgcaccaga aaggattgat
     9781 ggtgatactg gatgtggtct acaaccactt tggccccgag ggcaactacc tgtcgttgta
     9841 ttgcccgcag ttcttcaacg agcggcatca aacgccgtgg ggcgcggcca ttgatttcga
     9901 tgggcacgac agtcgcatcg tgcgcgagtt catcatccag aacgcgctgt attggctcga
     9961 agagttccat ctcgatggat tgcgcctgga tgccgtgcac gccattcgcg acgacagccc
    10021 cacacacctg ctgaccgagc tggcacaacg cgtgcgcgcc gccgaactac cgcgcgccgt
    10081 gcatctgatt cttgagaacg agcacaacga agcgatccgc ctgagcgcat cgctctatga
    10141 tgcgcagtgg aatgacgacg ttcaccacgc gctgcacgtg gcggcaagcg gtgaacgtga
    10201 aggctattac gccgactatg tcggcgacac cgagaagctc ggccgcgcgc tcgctcaggg
    10261 gtttgcctac cagggtgaaa tcatgccgta ccgtggctca gcgcgcggtg agccgagtgc
    10321 gaagttgccg cctcggcgtt tcgttgcctt cattcagaat cacgaccagg tgggcaatcg
    10381 cgccctcggt gagcggctcc accagatcgc cccatccgct gccgtaggcg cggtgactgc
    10441 ggtctatctg ctgctgccac agattccgat gctgttcatg ggtgaggagt ggcacgccgg
    10501 gcaaccgttt cagttcttct gcgacttcgg ggccgaactt gcgcaagccg tgcgcgacgg
    10561 acgccgccag gagttctcgc gcttcgcggc ttttcaatcc gagcaggcac gtgatcgcat
    10621 tcccgatccg cagtctgagc agacttttct ggacagcaag ctggactgga cggcattgga
    10681 gcaaccttcg cacgccagcg ccctgcgctg gtaccaggag ctactcgcgg cccgtcggcg
    10741 gcacgtggtt cccctgatct ctgcgatcgg acacggcggc cgttacacgg tgattgatca
    10801 tggcgcggtg atggtgcagt ggcaaagccc ggtcgccgaa ctgacgctgt tggcgaacct
    10861 gagtgcacgt cgtgtcaccg gattcccgga ggtgccgggg catctgctgt gggaacaagg
    10921 gcaaatcgag gcgagcggta cctatccgcc gtggtgcgtc cgctggatgc tgcgtacgtg
    10981 attccgcgcg ccacctatcg attgcagctc cacggggggt tcggcttcag tgctgcgacc
    11041 gattgcgtgc cgtaccttgc gcggcttggc gtgagccatg cgtacctgtc gccgtacctg
    11101 aaggcacgtg ctggcagtgc gcacggctat gacattgtcg accacagtca gctcaatccg
    11161 gaactgggcg atgaagaggc ctttcagcgc atgtcgcagg ccctgaaggc tcattcgctc
    11221 ggacacattc tggatttcgt accgaaccac atgggtgtgg gtggcgctga caatccgttg
    11281 tggcttgatg tgctggagtg gggaccggac gcgctgcatg ccggctggtt cgatgtggac
    11341 tgggacccgg accgacgcta tctgcacaac cggattctgg tgccgttcct cggcgatcag
    11401 tacggcatcg aactcgagcg cggcgtattg cggctgcgtt tcgatgagtc cgatggcagc
    11461 ttcgcggtgt gggcctacga tacgcacaag ctgccgatct ggccccctca ctacgcgcgc
    11521 atcctcacgg acgggactgc gcagctcgag cggctggccg atgctttcaa ctggctgccc
    11581 aactggcacc agcagatgcc gcagcgggcc gccgaactca aaacccgtct cgccgccctg
    11641 gtgcgcgatc acgccgatgc gcgtgaggcg gtgcaacgtg ccgtgcagcg cttcgagggc
    11701 cagcccgatc agagcggcag ctggcagtca ctggatgagc tgattgccga acaacgctgg
    11761 cgcatcgcgc acttcagagt ggcagccgac gacatcaact accggcgttt cttcaatatc
    11821 aacgatctgg ccggtctgcg catggagctg ccggaggtat tcgatcacgt ccatcagcgg
    11881 gtgctgcgct gggtgaagga cggtaccctc gacggacttc ggatcgatca catcgatggt
    11941 ctgtacgacc cgaaaggata cttgcgacgg ctgcagcggc gcctgagcac gcggcatgcc
    12001 gagcacggct tctacctggt ggtcgagaag atcctggccg cccatgagcc gctgcgcgag
    12061 gactggccca ttgacggcac taccggctat gacttcctca atcaggtact ggcgttgctg
    12121 atcgattcga gcgcgtacgc cgacttcgat cgatgctacc ggcaattcgt cggtgaaccc
    12181 caacgcttcg ccgacatcgt gcaccagtca aaacggcaca tcatggaaaa cgaaatggcc
    12241 agcgagctga acgtgctggc ccgggacatg ggccgactgg cgcgccagaa tccacgtacc
    12301 gccgacttca cgcgcaacct gctgcgacgg gcgatcaagg agctgatcgc ctgcttcccg
    12361 gtctaccgta cctacatcga tgacgccggc gagctcgaca gcgccgatca gcgcgatctg
    12421 agctgggcct ttgcccaggc gcgccgcaac gagtcggaac tcgatccgag cgtattcaac
    12481 ttcctggaac aggtgctgac cggtgccctg ctgcagcgct cacgcaccgg gttctcgcat
    12541 cagtcgttgc tgcgctgtgc gatgcgtctg cagcagtaca gcggtccggt catggccaaa
    12601 ggcgtcgagg atacggcctt ctatcgctat caccgcttca tcgcactgaa tgaagtcggc
    12661 ggcgatccgg agcgtttcgg cgcgacgctc tcgtccttcc acaagagcaa tcaggcccga
    12721 gcgcaccgct ggccgcacgc gatgctggcc acggcgacgc acgacaccaa gcggggcgaa
    12781 gacgcgcgtg cgcgcctggc agcgctgtcc gagttcgccg aagaatggat acagcagttg
    12841 ccggtctggt cacgcatcct gcgtggaccg ttgatcgccg aggacgcgcc actgaagccc
    12901 gaccgcaacg acgagtacct gctgctgcag ctgctgctgg gtacctggcc gtgcgaactg
    12961 atgacgagcg aggtgcgcga cgcccagttg tcaaaggact acgcgcagcg catacgcggc
    13021 gccatggtca aatcgatgcg cgaatcgcgc ctgcacaccg gctgggcatt tccgaacgcc
    13081 gagtatgagg aggccacgtt gggcatgatc gatgccgcac tgaccggcgc tcgctccagc
    13141 gcgtttctca gttcgttcct gccgttcgta cgaaaggtgg ccgactgcgg cgtggacaag
    13201 agcttgattc agacggtagt gaagctcacg tccccgggcg tgcccgacct ctacaacggc
    13261 agtgagctgt gggatctgtc gatggtcgat cctgacaacc gacggccggt ggactacgca
    13321 ctgcgaacac gcctgctacg gcagatcgat gagcgcctgg cggcagaccg cgcgctcggc
    13381 atgagcgaac tgcggcgcaa ctggtgcgat gcgggcatca aactggcgac gatcgcgacc
    13441 ctgctgcggc atcgtcgcga tcacgagagg ctgtacgcgg aaggcgacta tcagggactg
    13501 ccggcgagcg gtgcgcgggc ggatgatctg tgcgcctata cgcgccatcg caatggcgac
    13561 aagttgctgg tggccgctgt gcgtcgcgcc catcgcgcag acggcagcaa acagtgggaa
    13621 gacaccgtat tgcccgtgcc agaggctcta cgaagagagc aatggcatga gctgctcacc
    13681 ggtcgcctcg tcgcgctgcg agaggacacc gtcggccccg ccgatctgtt cgccgagcta
    13741 ccggttgtcg tgctcgcctc gaacggccac tagcctggcg gtgaggccag cagatcctga
    13801 tactcggggt gacgctcgat gaaggtccgg acgtacgggc agcgcgggat caccttgatg
    13861 cgctgagagc gcaccagatc cagtgcgccg ctggtgaggc gtgcagcgac gccgccgccg
    13921 cgcagcgccg ccggcacttc ggcgtgggtg agggtgcgta ccctgccggc agcggtgtaa
    13981 tcgatgaagg cggtaccacc gtcgagcttc atctcgtagc ggctctcagc cgcattgtcc
    14041 agcacctggt cgcccatgat cgatcacacc tcgagcgctt cttcggccgc gttgaactgc
    14101 agggctgcca gcttggcata gagcgggctg ccgtgcatca gctcggcatg cttgccgatc
    14161 gcaacgatcc ttccggcctg cagtaccacg atgcggtcgg cgcgcagcac ggtcgacagg
    14221 cggtgagcga tgaccagcgt cgtacgcccg cgcatcagca cgttcaacgc cgcctgcacc
    14281 agtcgctcgg attccgcatc gagcgaactg gtggcctcat ccagtagcag gatcggcgga
    14341 tccttcagga tggcgcgcgc aatggcgaca cgctgccgtt gcccgccgga cagacgcacg
    14401 ccacgctcac ccagataggt gttgtagccc tcgggcagct cgcgcagaaa ctcatcggct
    14461 gccgcggcgc tggccgcagc gacgatctgc tcatcggtgg atccaggccg gccgtagtcg
    14521 atgttctcgc gcgcggttgc gccgaacagc accgtatcct gcggcacgaa gccgatcttc
    14581 gagcgcagct cctcaggccg ccaggtccgg atatcgatgc catcgatgct gatgcacccg
    14641 gcctgcggat catagaagcg cagcagcagc tgaaaggtcg tgctcttgcc ggcgccggac
    14701 ggtccgacga aggcgaccgt ctccccggga cgcacttcca gtgaatactc gcgcagcgcc
    14761 cgcgtgtcgc tgcgcgacgg atacgagaac gacacattct cgaacgcgat gcgtccgctg
    14821 atcttcggcg gcaactccgc cggctgc
//
LOCUS       DATMRM010000138         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_391871, whole genome shotgun sequence.
ACCESSION   DATMRM010000138 DATMRM010000000
VERSION     DATMRM010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391871"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VNZ06_04120"
     CDS             <1..496
                     /locus_tag="VNZ06_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19968.1"
                     /translation="SQRDAVKADEDRRRQIDEAQASANDIVPKAEVAAQRQIADAEVY
                     AKQTVAIAEGEAERFSQVAAAYAQAPEVTRNHIYTETIEGILSRSRKIIVDTKGSGGS
                     LIYIPLDKLAEAIKATSASAPPAAAASNNGSGSTTSASTASNSNAGADRSDSDERARE
                     RPDR"
     gene            497..1360
                     /locus_tag="VNZ06_04125"
     CDS             497..1360
                     /locus_tag="VNZ06_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HWW19969.1"
                     /translation="MATRIMTAVGLLLVAVLLWRSVYVVDEGEVALRTRLGHIEDSNG
                     GPGLHLKSPFDEVHIFDRRILSHTYPGEPFLTRDQKAVNVDFYIKWRLIDALRFYQST
                     GGDQDAAAQQLGDWVRERLKSAVAGQPLSSVSAQARGALTEAAFEELQQAAERLGLDL
                     IDVQLQRVELTEDVASVVYQRMRQGLIAKAAQLRASGASEAEKIRGEAERRRADVLAN
                     ATRQAQHLRGEADAQAAADLAKAYGRNTDFAAFYRSMQAYKNTLGRDGDILVISPEGE
                     FFKYLHSASGR"
     gene            1382..1579
                     /locus_tag="VNZ06_04130"
     CDS             1382..1579
                     /locus_tag="VNZ06_04130"
                     /inference="COORDINATES: protein motif:HMM:NF021366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HWW19970.1"
                     /translation="MGLDWTDLCAALAIFCIIEGVMPFINPSGVKRLLSRIASLEDRE
                     MRVAGLVSMAIGLTILFLVRS"
     gene            1579..2874
                     /locus_tag="VNZ06_04135"
     CDS             1579..2874
                     /locus_tag="VNZ06_04135"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HWW19971.1"
                     /translation="MGRFVVVIGTQWGDEGKGKLVDLLTERATAVARFQGGHNAGHTL
                     VIGGVKTVLSLLPAGILREGVRCFIGNGVVLALEALFREADGLIAQGVPVLERLRISP
                     ACPLILPSHVALDRARETARGENAIGTTRRGIGPAYEDKVARRAVRVADLYQRDRFAA
                     KLGEVLDFHNFVLQRYFSQSPVDFQRTLDEQLEFAERLRPLVFDVTGALAQLRARKAN
                     VLFEGAQGAMLDVDVGSYPFVTSSNTTAGGAATGTGVGPCMFDGVLGIVKAYTTRVGA
                     GPFPTELFDEYGEHLSRVGHEFGAVTGRRRRCGWFDAVALRRSIVHSSVSSLCMTKLD
                     VLDGLDTIRICVGYRMGGQTLSEPPLFSDAASELEPIYEDQPGWKESTVGVTNLESLP
                     KNARAYLQRIERLVGVPLDLISTGPDRTQTIILHHPFDD"
     gene            3033..>3911
                     /locus_tag="VNZ06_04140"
     CDS             3033..>3911
                     /locus_tag="VNZ06_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWW19972.1"
                     /translation="MDDLAQPPIETAPEEEVLGVPDLGMLRTLLGNLDGMLFRYRNDA
                     DFTLEFVSEGCMRLTGYPAASLLDPRKTRFAELIHSADRARWQQASAQALQARERYAV
                     EYRIVRADGAIRWVSERGMAQLNRDADTAVFEGFIEDVTERKRVEEALEELELRCQSM
                     FENALEGMFRASADGQWLNANRALARIYGYDSPEELLRSMHDTSRAELRRRLEVDGDV
                     NGFECQATKQNGEVIWVSMTGRAQTDADGRVTHYEGTVEDVTERRRFQAHIEHQANFD
                     ALTGLANRALLDDRLRQ"
BASE COUNT          731 a         1153 c         1299 g          728 t
ORIGIN      
        1 gtcgcagcgc gatgcggtga aggccgatga agaccgtcgc cgacagatcg atgaggcgca
       61 ggccagcgcg aacgacattg tccccaaggc ggaagtcgcc gctcaacgcc agatcgcgga
      121 cgcggaggtg tatgccaagc agacggtggc gattgccgag ggtgaagccg aacgcttcag
      181 ccaggtggct gccgcctatg cgcaggcacc ggaagtcacc cgcaatcata tctataccga
      241 gaccatcgaa ggtatcctgt cgcgctcgcg caagatcatc gtcgatacca aaggtagcgg
      301 cggcagtctc atttatatcc cgctcgacaa gctggccgaa gccatcaagg ccacgagcgc
      361 ttcagccccg ccggccgcag cggccagcaa caacggcagc ggcagtacga cgagcgcttc
      421 cactgcttca aacagcaacg ccggcgccga tcgctccgac agcgacgagc gcgcgcgtga
      481 gcgcccggat cgatagatgg caacgcgcat catgaccgcg gtgggactgc tattggtggc
      541 cgtattgcta tggcgctcgg tctatgtcgt ggacgaaggc gaagtggcac tgcgcacgcg
      601 actggggcat atcgaggaca gcaacggcgg accgggtctg catctcaagt cgccgttcga
      661 tgaggtgcac atcttcgatc ggcgcatcct gtcgcacacc tacccagggg agccctttct
      721 gacgcgcgat cagaaagcgg taaatgtgga cttctacatc aaatggcgtc tgatcgacgc
      781 gctgcggttc taccagagca ccggtggcga tcaggatgcg gccgcgcagc agctcggcga
      841 ttgggtgcgt gagaggctca agagcgcagt ggccggccag cctttgagca gcgtcagcgc
      901 gcaagcgcgc ggtgccctga ccgaggccgc gttcgaggag ttgcagcagg cagccgagag
      961 gctcgggctt gatctgatcg atgtgcagct gcagcgggtt gaactcacgg aggacgtggc
     1021 cagcgtcgtg tatcagcgta tgcggcaggg cctgattgcc aaagccgcgc agctgcgggc
     1081 gtcgggcgcc tcggaggccg aaaagatacg cggtgaggcg gagcggcggc gcgccgatgt
     1141 actggccaac gccactcgtc aggcgcagca cctgcgtggc gaagccgatg cacaggcagc
     1201 ggccgacctg gccaaggcgt acggacgtaa caccgacttt gctgctttct accgcagcat
     1261 gcaggcgtac aagaacacgc tgggtcgcga cggcgacatt ctggtcattt cgccggaagg
     1321 cgagttcttc aaatatctac atagcgccag cgggcgctga attttcaagt caggggctct
     1381 cgtggggttg gactggactg atctgtgtgc tgcgttggcg attttttgca tcatcgaagg
     1441 cgtcatgccc ttcatcaacc cgtcaggggt gaagcgctta ctgtcgcgga tagcctctct
     1501 ggaggatcgt gagatgcgcg tggcgggatt ggtgagcatg gcgatcggtt tgacgatcct
     1561 gtttctggtg cgttcctgat gggacgcttc gtcgtcgtca tcgggaccca atggggtgat
     1621 gaaggcaagg gcaagctggt cgacctgctg accgagcggg ccaccgccgt cgcgcgtttt
     1681 cagggcggcc acaatgccgg gcatacgctg gtcatcggcg gggtcaagac cgtgttgtcg
     1741 ttgttgcccg ccgggatcct gcgcgaaggc gtgcggtgct tcattggcaa cggggtggtg
     1801 ctggcgctcg aagcgctgtt tcgcgaagcc gacggcctga tcgcgcaggg cgtgccggtg
     1861 ctggagcgtc tgcgcatttc tccggcctgc cctctgattc tgccgtcgca tgtcgcactg
     1921 gaccgggccc gcgaaactgc ccgcggcgag aatgcgatcg gtaccacgcg ccgcggtatc
     1981 gggccggcct atgaggacaa agtcgcgcgc cgtgcagtgc gcgtcgcgga tctctaccag
     2041 cgcgaccgtt tcgcagcgaa actcggcgaa gtgctcgatt ttcacaattt cgtgctgcag
     2101 cgttacttct cacagtcgcc ggtagacttt cagcgcacac tcgatgagca gctggagttt
     2161 gcggagcgct tgcgaccttt agtgttcgat gtcaccgggg ccctggcgca gctacgggcg
     2221 cgcaaggcca acgtattgtt cgaaggtgcg cagggcgcga tgctcgatgt cgatgtcggt
     2281 agctatccat tcgtcacctc atccaatacc accgcgggcg gcgcggccac gggtaccgga
     2341 gtgggcccgt gcatgttcga tggcgtgctg ggtatcgtga aggcttacac cacacgggtg
     2401 ggagccggcc cattcccgac cgagttgttc gacgaatacg gcgaacacct ctcgcgggtg
     2461 ggtcacgaat tcggggccgt gaccggccgt cggcgccgat gcggctggtt cgatgctgtg
     2521 gcgctgcgtc gatcgatcgt acattccagt gtctccagtc tgtgcatgac caagctggat
     2581 gtgctggacg gactggacac gatccggatc tgtgtcggct atcggatggg agggcagacg
     2641 ctgtccgagc cgccactgtt ttccgacgcg gcgtcggagc tagagccgat ctacgaagac
     2701 cagcccggct ggaaagaatc cactgtcggc gtcacgaatc tggagtctct gccgaagaat
     2761 gctcgcgcct atctgcagcg catcgaacgg ctggtaggag tgccgttgga cctgatttcc
     2821 accggtcccg accgaactca gaccatcatt ctgcatcatc cgttcgacga ctgagcgcgg
     2881 tccgcgcccg gatgcgactt attcaattgg tcgcgttcac agttagcaaa tagtcaccta
     2941 gttggtcatc ccagccatct caagtttccg cctcgttagc tcgatatgcc tcagaccgac
     3001 aaggaaaatt tgtctctgca tcaagttaca taatggacga tctcgcgcag cccccgatcg
     3061 agacagcgcc tgaggaggaa gtactcgggg tgcccgattt ggggatgctg cgcaccctgc
     3121 tgggcaatct cgacggcatg ctgtttcgct accgcaacga cgccgacttc accttggaat
     3181 tcgtgagtga aggttgcatg aggctgaccg gttatccggc cgcgagcctg ctcgaccctc
     3241 gcaagacgcg ctttgccgag ctgatccatt ccgcggatcg tgcgaggtgg cagcaggcta
     3301 gcgcgcaggc cctgcaggcg cgagagcgct atgcggtcga atatcgcatc gtgcgcgctg
     3361 acggggcgat tcgctgggtc agcgagcgcg ggatggcgca gctcaaccgc gacgccgaca
     3421 cggcggtgtt tgaaggcttc atcgaagatg tcactgaacg caagcgcgtc gaggaagccc
     3481 tcgaagagct cgagctgcgc tgtcagagca tgttcgagaa cgcgctcgag ggcatgttcc
     3541 gcgccagcgc ggacggccag tggctgaacg ccaatcgcgc gctggcgcgt atctatggtt
     3601 acgactcgcc ggaagaactg ctgcgctcga tgcacgatac ttcgcgcgcg gagctgcgcc
     3661 ggcggctgga agtcgatggc gatgtcaatg gttttgaatg tcaggccacc aagcagaatg
     3721 gcgaggtgat ctgggtctcg atgaccggcc gggcgcagac cgatgccgac ggacgggtca
     3781 cgcactacga aggcacggtg gaggatgtca ccgaacgacg gcgtttccag gcgcacatcg
     3841 aacatcaggc caatttcgac gcgctgaccg gcctcgccaa ccgggcgttg ctcgacgacc
     3901 ggctgcgaca g
//
LOCUS       DATMRM010000139         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2854862, whole genome shotgun sequence.
ACCESSION   DATMRM010000139 DATMRM010000000
VERSION     DATMRM010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2854862"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..328
                     /locus_tag="VNZ06_04145"
     CDS             59..328
                     /locus_tag="VNZ06_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HWW19973.1"
                     /translation="MSAQPCAHLAAITTVKHAKRRVCEECIKTGDRWVHLRTCQECGV
                     TLCCDSSPNRHASKHAHATKHPVIASAELGERWLYCYPDQQVAEY"
     gene            complement(346..1755)
                     /locus_tag="VNZ06_04150"
     CDS             complement(346..1755)
                     /locus_tag="VNZ06_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWW19974.1"
                     /translation="MIAASHLLTGALLLLLLHVGCASVAAIHALLTKSDPRSAFGWIV
                     VCWLFPLVGSLLYAFFGINRVRARARQLRFGLPTMRDACPPAPSDEPVAQLTRIGDAL
                     TQWSRVPGNAIEPLENGENAFPAMLKAIAAARQSVWLATYIFETDPVGRGFIAALACA
                     AARGVEVRALIDGVGEWYSWPHAVPLLRRAGVRAARFLAPRLLPWPRLAINLRNHRKL
                     LIVDGQIGFLGGMNIGGREVGKAAHRRMADLHFQARGPIVAQLARAFAIDWQFAAAEE
                     LQIAAAPEATGPAVCRAITDGPDEDNDKLLYVILGALSVARHQILVMTPYFIPQPELT
                     AALQAAALRGVEVCLVLPERSNLRYVDWASRRWWPPLLARGVRIYLQPAPFSHTKLLI
                     VDGLYAQIGSANMDLRSLRLNFEIAVEIYCADTCQQLVAYVLAARARSAEFTLPQALA
                     QSWLVRARNSLLWLLSPYL"
     gene            complement(2085..>2678)
                     /locus_tag="VNZ06_04155"
     CDS             complement(2085..>2678)
                     /locus_tag="VNZ06_04155"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWW19975.1"
                     /translation="IQEWWGVNGQIRGVAERLASAGYIALVPDLFRGKSTAEAEEAHH
                     LMTGLNFGDAASQDVRGAVQFLKKRSGTVGVTGFCMGGALTLLALCMAPEIDAAVVWY
                     GCPPLEYIDATKIKAPLQAHWATQDQFFPIATIDLLEKKLREAAVDFDFHRYLAHHAF
                     ANETAVGPGRIPATQYDPVWSQQAWDRTFNFFGHWLG"
BASE COUNT          531 a          874 c          824 g          449 t
ORIGIN      
        1 ccggccactg aacgttcacc tgctcttcac cgctcagcct gcactggagg agtagaacat
       61 gtccgctcaa ccctgtgctc atctggccgc gatcaccacg gtgaaacatg ccaagcgccg
      121 cgtgtgcgag gagtgcatca agaccggcga ccggtgggtg catctgcgca cctgtcagga
      181 atgcggcgtc acgctgtgct gcgatagctc gcccaaccgc cacgcgagca agcacgcgca
      241 cgctaccaag catcccgtca tcgcttcggc agagctcggt gagcgctggc tttactgcta
      301 cccagaccag caggtggcgg agtattgagg cctctcgcag gcgctctaca gataaggcga
      361 cagcagccag agaaggctgt tgcgcgcacg cacaagccac gactgcgcga gcgcctgcgg
      421 caatgtgaac tcggcggacc tcgcccgagc cgccagaacg tacgcgacca gttgctggca
      481 agtgtcggcg cagtagattt cgactgcgat ctcgaagttc aggcgcagac tgcgcagatc
      541 catgttcgcc gatccgatct gcgcatagag cccgtccacg ataagcagct tcgtgtgcga
      601 aaacggcgcg ggttgcagat atatcctcac ccctcttgcg agcagtggtg gccaccaacg
      661 cctcgaggcc caatccacgt agcgcagatt gctccgctct ggcagcacga ggcatacctc
      721 aacgccgcgc agcgccgcgg cctgcagcgc cgcggtcagc tcaggctgcg ggataaaata
      781 gggcgtcatc accagaatct ggtgccgtgc gacggagagc gctcccagta tcacgtagag
      841 cagtttgtcg ttgtcttcgt ccggcccgtc ggtgatggct ctgcaaaccg cagggcccgt
      901 cgcttcgggc gcggcggcga tctgcagctc ctccgccgcg gcgaattgcc aatcgatcgc
      961 gaaggcgcgc gcgagctgcg cgacgattgg accgcgcgct tgaaagtgca gatccgccat
     1021 acggcggtgc gcagctttac cgacctcacg accccctata ttcatgccgc cgagaaaccc
     1081 gatctgaccg tcgacgatca ggagcttgcg gtggttgcgc aagttgatcg ccaatcttgg
     1141 ccagggcagc agccgcggag cgaggaagcg cgccgcgcgc acgccggcac ggcgcagcaa
     1201 cggcaccgca tgcggccagc tataccattc acccacaccg tcaatcagcg cacgcacctc
     1261 gacgccacga gccgccgcgc acgcaagcgc ggcgatgaat ccccgaccca ccggatcagt
     1321 ctcgaagatg taggtcgcga gccataccga ctgccgtgca gcggcgatgg ctttgagcat
     1381 cgccgggaag gcgttttccc cattctcgag tggctcgatg gcattcccag gaacacggga
     1441 ccactgggtt agcgcatcgc cgatccgggt cagttgcgcc actggctcat cagagggcgc
     1501 aggcggacac gcgtcacgca tcgtcgggag cccaaaacgc aattgccgcg cgcgtgcgcg
     1561 cacgcggttg atgccgaaga acgcgtacag cagcgagccc accaggggaa acagccagca
     1621 gaccacgatc cagccaaacg cgctgcgtgg gtccgacttg gtcagaagcg catgaattgc
     1681 ggctactgaa gcgcatccca cgtgaagcag aagcagaagc agcgcgccgg taaggaggtg
     1741 ggacgcggct atcacaagcg tttaatcggc atgaactcag tccctgcagg cgctcaccgg
     1801 cgaactttgg cgcccttgat cattggaata ggtcttgggg cagcgcgcga gcgagtcacc
     1861 gtaacccatc actcgattct cgttcaagaa tcagcggtaa cggagacagc gattacaccg
     1921 ctgcctgcaa aggcggcaat cgctatcaca cctttcgtcg actcgggcgg gcgcgttggg
     1981 ccaagaaact ctgagcgcgg ctcgcgcgcg atcctcacct ttcaagggcg attcgactcg
     2041 tactgcctca tgggaatggt gcccgcacga ccctcttcat cgcgttagcc cagccagtgg
     2101 ccaaagaagt tgaaggtccg gtcccacgcc tgttgcgacc agacggggtc gtattgggtg
     2161 gctgggatcc gtcctggccc aacggccgtc tcgttcgcga aggcatggtg tgcgaggtag
     2221 cgatggaaat caaagtccac tgccgcctca cgcagcttct tctccagcag atcgatcgtg
     2281 gcgatcggga agaactgatc ctgcgtggcc cagtgcgcct gtagcggcgc tttgatcttg
     2341 gtggcgtcga tgtactccag cggcgggcag ccgtaccaga ccacggcggc atcgatctcc
     2401 ggggccatgc aaagagccag gagcgtcaag gcgcctccca tgcaaaagcc cgtgacgccc
     2461 accgtgccgc tgcgcttctt gaggaactgc accgcgccac gtacatcctg tgacgcggca
     2521 tcgccaaagt taagacccgt catcaggtgg tgcgcctcct cggcttcggc cgtggacttg
     2581 ccgcggaata ggtccgggac gagagcgatg taacctgcgc tggccagtcg ctcggcaacg
     2641 ccgcggattt gtccgttgac cccccaccac tcctggat
//
LOCUS       DATMRM010000140         7629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2856461, whole genome shotgun sequence.
ACCESSION   DATMRM010000140 DATMRM010000000
VERSION     DATMRM010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2856461"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2656
                     /locus_tag="VNZ06_04160"
     CDS             <1..2656
                     /locus_tag="VNZ06_04160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HWW19976.1"
                     /translation="PATGTGARSGNVERSASAASGTTAPPARASSAAPSSTGGGGSPY
                     TVRNGDTLSAVAAQNYPGSDRAARERQLVAIYRANPNAFEGNMNLLRAGSKLQLPGDA
                     ELQEISPGEASAEVRKQYQSWSGARAAAPSGSSGQLHLVPPQESAAAPSNAPSSPSAT
                     APASNAPSGAASTAANQALQSRVQQLESQLADSQRALQARNDELAQLQAQVRAQAQAN
                     KAAPPAPAPVTPPAPTATPKAAAPPVQPAPAPAPVAVPAPSAETPPAAQPAPEPAPEA
                     AKPKPAVAAKAPPAGTSFLDLIEEYWYVPVALILIVLVLLVLRFVRARQEDEFDRSLG
                     RLHPAFEGTATSSALRPVDTMPVRTLPASAGEQSYRVEESGAHEQPTAVLHDRTTEGP
                     AVTTGEHVVIEDTVTGEHPVALDQGDPLAEADFHMAYGLYDQAADLVQIAINREPNRR
                     DLRLKLLEVFFVWGNKERFLQTARELAGTRDQALPGEWEKVVIMGRQIAPEDALFSSA
                     GALSGAASGGVDLNLEGGQNRVDFDLLGEPTASPEAAANGVGLDLDLGAALGDTPAAA
                     ESDKLGDSGVDFVLDDPERGNDATGSTREMPEAPPRPGSGATATMQVINTGTAPSDAP
                     TVEQPQLRGGENATIREKIDAAARGGVTADQTAELALDDLGLDLGALEGSSDETILQG
                     GPDAPTMLAGMDEETRRLLSSAEQEDQDRTQITPAPPSTSESGTWLFTDADLTDLVPE
                     EDRKRLSGDEAAPTQVVTQITQPPPHVDPSATGRMQTLNASGLDLDLGNLGENKSANG
                     IDLDVGSPAVGEGTFVQTQKIAQADVPLPELEPATMSEVGTKLDLARAYMDMGDPEGA
                     RSILAEVLAEGSVSQKQEARRLMDTLPG"
     gene            2684..3520
                     /gene="truA"
                     /locus_tag="VNZ06_04165"
     CDS             2684..3520
                     /gene="truA"
                     /locus_tag="VNZ06_04165"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWW19977.1"
                     /translation="MRRTTDVSRPGRVAVGVEYEGGAYSGWQQQRTANSIAATLERAL
                     SSIADEPVEVICAGRTDAGVHAREQVAHFDTHATRSRIAWLLGTNTELPADVSLRWVH
                     AVPDHFHARYSALSRTYRYLILNQAARSALLAGRALLVHQPLDVEAMQAGAQWLVGEH
                     DFSAFRSSECQSHSPIRLLQSLRVRRNGPWVAIDVTGNAFLHHMVRNIAGLLLLIGQG
                     RSPPERAREQLESRQRSTGAATAAAHGLYLWHVQYPPQFGLPTDSAMIDAAIPGWPSA
                     VP"
     gene            3534..4400
                     /gene="accD"
                     /locus_tag="VNZ06_04170"
     CDS             3534..4400
                     /gene="accD"
                     /locus_tag="VNZ06_04170"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HWW19978.1"
                     /translation="MSWFEKIVPSKIRTERRSRSVPEGLWIKCPACDAVLYRAELERN
                     LNVCPKCSNHMRIGARERLRALLDPEPLVEIGADVMPEDPLKFKDSKRYKDRLAQAQK
                     TTRENDALIALEGSLHGEALVACAFEFNFLGGSMGSVVGERFKRSVDRAIARRCPLVC
                     FTATGGARMQEALLSLMQMAKTSAALARLARERLPFVSVMTDPTTGGVSASLAMLGDL
                     NIAEPHALIGFAGPRVIQQTVRETLPEGFQRSEFLLEHGGLDLIVDRRDMRDRLAQLL
                     RLLQKRPPLQAA"
     gene            4397..5668
                     /gene="folC"
                     /locus_tag="VNZ06_04175"
     CDS             4397..5668
                     /gene="folC"
                     /locus_tag="VNZ06_04175"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF008101.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HWW19979.1"
                     /translation="MRSLADWLSFQQRAHPRAVDLSLERVCSVAARLGLLQRQALLAT
                     VAGTNGKGSVATTLACLLQSCGQRVGLFTSPHLVRYNERIRIDGEAASDSDLVSAFEH
                     IESARGEVTLTFFEYNALAALELFRRARVEAMVLEVGLGGRLDATNLLSADVAVVCSI
                     GFDHRDWLGNTLEQIGAEKAGIFRPGQKVVLGSPEMPQSVWQAASALGCAVWTAEREF
                     SWSLQPSPDAPPAWNYHSARCELSALPAPALAGHIQYRNAASALTALQLLDVPHRCER
                     SRVIAGLERVLLPGRFQIVPGEVEWILDVAHNEPAAQVLAQSLRERGAAGRTWAVFGT
                     LNDKDSAAIALTLDAQMDHWVLAGIDDPRGLSAQALRERLSSLRAEVELADDVISACE
                     RVRARAHAGDRVVVLGSFHVVGPALAWLGLY"
     gene            5682..6356
                     /locus_tag="VNZ06_04180"
     CDS             5682..6356
                     /locus_tag="VNZ06_04180"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HWW19980.1"
                     /translation="METRLKQRLTGAAIIVALIVALVPEMFRGQAGAPPANTASSSSG
                     EGPPVRTYTIDLSNNPKNGPLQSANEAPPASAPAASGSSSPAAPVESPPAPAPAAAVA
                     APRAAPSSTPPPSPPPSHGSAPAPPHATRAAPPVSSPAAAPARTATAPTGNWSVQLGL
                     FSKRENAERMISEAKTKGFNPAIAGPDAKGQFHVHVTGLTDRVAAQAMAQKLKGEGLP
                     AAVVAP"
     gene            6431..6934
                     /locus_tag="VNZ06_04185"
     CDS             6431..6934
                     /locus_tag="VNZ06_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HWW19981.1"
                     /translation="MNWFDYLLIVVMVLSLIAGLMRGLLREAIGLITWVVAIWIAFHY
                     AAQLEPYLGGLLANDSLRPWIARSLIFLVVLLIGTTLAAVISHFIRVSMFAGMDRLWG
                     ALFGIVRGMVVIGAILILCHGTRLQTETWYRASVLAPYGEHVANILRALVGERKITVE
                     PSVSSAS"
     gene            6958..>7629
                     /locus_tag="VNZ06_04190"
     CDS             6958..>7629
                     /locus_tag="VNZ06_04190"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011060388.1"
                     /note="Catalyzes first step of the de novo purine
                     nucleotide biosynthetic pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWW19982.1"
                     /translation="MCGIVGIVGTSPVNQRLYDALTVLQHRGQDAAGIMTAHEGRLCS
                     RKGPGLVRDVFEQHHMLELQGNVGIGHVRYPTAGCDSASEAQPFYVNAPYGICLAHNG
                     NLTNAVELAEVMIREDRRHLNTESDSEVLLNIFASELQRVGTPRVTPADIFAAASAVY
                     RRCRGGYAVVAMVIGHGILGFRDPNGIRPLVLGSRRTARGTEYMLASESVALAMLSFE
                     LVRDVA"
BASE COUNT         1311 a         2494 c         2543 g         1281 t
ORIGIN      
        1 gccggcgacc ggtaccggtg cccgcagcgg caacgtcgaa cgatcggcca gcgccgcctc
       61 cggcacgacc gctccccccg cgcgcgcctc atcggccgct cccagcagca ccggcggcgg
      121 tggcagcccc tacacggttc gtaacggcga cacgctctcc gccgtggccg cgcaaaacta
      181 tcccggcagc gaccgcgccg cacgtgagcg ccagctggta gcgatctacc gcgccaatcc
      241 gaatgccttc gaaggcaaca tgaacctgct gcgggcgggc agcaaactgc agctgcccgg
      301 agatgccgag ctgcaggaga tcagccccgg tgaagcgtcg gcggaagtgc gcaagcaata
      361 ccagagctgg agcggtgcgc gggccgcggc accgtccggg tcatccggac agctgcatct
      421 ggtgcctccg caggaatcgg ccgctgcgcc cagcaacgcg ccgtcgagcc cctcggccac
      481 agcgcctgct tccaatgccc cctcgggcgc cgcctcgacg gcggccaacc aggcattgca
      541 gtcgcgcgtt cagcagctgg aatcacagct ggcggactct cagcgcgcgc ttcaggcgcg
      601 caacgatgag ctggcgcaac tgcaggcgca ggtgcgcgcc caggcacaag ccaacaaggc
      661 cgcgccgcct gctccggcgc cggtgacgcc tccggcgcca actgcgacgc cgaaggctgc
      721 cgcgccgcct gtacagccgg cacctgcccc ggcacccgtc gccgttccgg cgccgagcgc
      781 cgaaacgccc ccggcagcgc agcccgcgcc ggagccagcc ccggaggcgg caaagccgaa
      841 gccggccgtg gctgcgaagg cgccaccggc gggcacctcg ttcctcgatc tgatcgagga
      901 gtactggtac gtaccggttg ccctgattct gattgtgctc gtgctgctgg tgttgcgctt
      961 tgtacgcgcc cggcaggagg acgagttcga ccgttcactc ggacgcttgc atccggcctt
     1021 cgaaggcacc gccacctcct cggcattgcg gccggtggac acgatgccgg tgcgcacact
     1081 gccggcctcg gccggggagc agtcttatcg ggttgaagaa tctggcgccc acgagcagcc
     1141 gacagcggtc ctgcacgatc gcaccacgga aggcccggcg gtcacgaccg gcgaacatgt
     1201 cgtcatcgag gacaccgtta ccggcgagca tccggtggcc ctggatcagg gtgatccgct
     1261 cgcggaagcc gatttccaca tggcctacgg cctgtacgat caggcggccg acctggtgca
     1321 gatcgccatt aaccgcgaac ccaatcggcg cgacctcaga ctgaagctgc tcgaagtctt
     1381 cttcgtctgg ggcaacaaag agcgcttcct gcagaccgcg cgcgagctgg ccggcacgcg
     1441 cgatcaggcg ctcccgggcg agtgggagaa agtcgtgatc atgggccggc agattgcgcc
     1501 cgaggatgcg ctgttctcca gcgctggcgc cctgtcgggc gcggccagcg gcggagtcga
     1561 tctcaatctc gagggcggcc agaaccgcgt ggatttcgat ctgctcggtg agcccaccgc
     1621 cagtcctgag gctgcggcaa atggggtcgg gctcgatctg gatctcggag cggcgctcgg
     1681 cgacacgccc gccgcggcgg aatccgacaa gctcggcgac agtggcgtgg acttcgtact
     1741 ggatgatccc gagcgcggca acgatgccac cggcagcacg cgtgaaatgc ctgaggcacc
     1801 accgcgtccc ggcagcggtg ccacggccac catgcaggtc atcaataccg gcaccgcgcc
     1861 atcggatgcg cccacggtcg aacagccgca gctgcgcggc ggagagaatg cgaccatccg
     1921 cgagaagatc gatgcggcgg cacgcggtgg cgtgacggcc gatcagaccg cggagctggc
     1981 actggatgat ctgggactgg atctcggagc gctcgaaggc agcagcgacg aaaccattct
     2041 gcagggcggg ccggatgcac ccacgatgct ggcgggaatg gacgaggaga cccgtcgtct
     2101 gctttccagc gcggaacagg aagatcagga tcgcactcag atcacgcccg ctccgcccag
     2161 taccagcgag agcggcacct ggctgttcac cgatgccgac ctgaccgatc tggtgcccga
     2221 ggaggatcgc aaacgcttga gtggcgatga ggccgcgccg actcaggtcg tcacgcagat
     2281 cacgcagcca ccgccgcatg tggatccgag tgcgaccggc cggatgcaga ccctcaatgc
     2341 gagcggactg gatctggatc tgggcaatct gggcgagaac aaatccgcca atggcatcga
     2401 tctggatgtc ggctcgccgg ccgtgggcga gggtaccttt gttcagacgc agaaaattgc
     2461 gcaggccgat gtaccgctgc cggaactcga gcccgcgacg atgagtgagg ttggaaccaa
     2521 gctcgatctg gcacgcgcgt atatggacat gggcgatccg gaaggtgcgc gcagcatcct
     2581 ggccgaagtg ctcgccgaag gctcggtgtc gcagaagcag gaagcgcggc gtctgatgga
     2641 cacgctgcct ggttaaaccc gcgctgttga cacccagtgc ctcgtgcgac gcacgaccga
     2701 tgtatcgcgc cctggacgcg tcgcggttgg cgtcgagtac gaggggggtg cctacagcgg
     2761 ctggcagcag cagcgtaccg ccaacagcat cgcggcgacg ctcgaacgcg ccctgagttc
     2821 gatcgctgac gagcctgttg aagtcatctg cgcagggcga accgatgccg gcgtgcacgc
     2881 gcgcgagcag gtggcgcatt tcgataccca cgccacgcgt agcaggatcg catggcttct
     2941 cggcaccaac accgaactgc cagccgacgt gagcctgcgc tgggttcatg cggtgccgga
     3001 tcactttcat gcgcgctaca gcgcgctgtc gcgtacctat cgctacctga tcctgaatca
     3061 ggccgcgcgt tcggcgctgc ttgccggacg ggcgttgctg gtacaccagc cgctcgatgt
     3121 cgaggcgatg caggcgggcg cgcagtggct cgtcggcgag catgacttca gcgcctttcg
     3181 ttcctccgaa tgccagtcgc attcgccgat acggctgctg caatccctgc gtgttcggcg
     3241 caacggccca tgggtcgcga tcgacgtgac cggcaacgcg ttcctgcatc acatggtgcg
     3301 gaacatcgcc ggcctgctgc tgctgatcgg gcaggggcgc tcaccgccgg agagggcacg
     3361 cgagcagctc gagtcgcggc agcgcagcac cggggcggcg accgccgcgg cccatggttt
     3421 gtacctctgg catgtgcaat atccccctca gttcgggctg ccgacggatt cagctatgat
     3481 cgacgctgcg attcccggct ggccgtctgc agtgccatag ggttgaaaag ccaatgtcgt
     3541 ggtttgaaaa gatcgttcct tcgaagatca ggaccgagcg ccgctcgcgt tcggtgcctg
     3601 aaggcctgtg gatcaagtgt ccggcctgcg acgcggtgct ctatcgcgcc gagctggaac
     3661 gcaatctgaa cgtctgcccc aagtgcagca atcacatgcg catcggcgcg cgcgagcggc
     3721 tccgggcgct gctcgatccg gaaccgctgg tggaaatcgg cgccgacgtg atgccggagg
     3781 atccgctcaa gttcaaggac agcaaacgct acaaggaccg cctggcgcag gcccagaaga
     3841 ccacccgcga gaatgatgcg ctgatcgcgc tcgaggggag tctgcacggc gaagcgctgg
     3901 tggcctgtgc cttcgaattc aactttctcg gcggctcgat gggatcggtg gtcggtgagc
     3961 gcttcaagcg ctccgtcgat cgtgcgattg cgcgacgctg tccgctggtg tgcttcaccg
     4021 ccaccggcgg ggcccgtatg caggaagccc tgctgtcgct gatgcagatg gcgaaaacct
     4081 ccgccgcgct ggcgcgactg gcgcgtgagc gcttgccgtt cgtctcggtg atgaccgatc
     4141 cgacgaccgg cggtgtgtcg gcaagtctgg cgatgctggg ggatctcaat attgccgagc
     4201 cgcacgcgct gatcggcttc gccggccccc gcgtcattca gcagaccgtg cgtgagacac
     4261 tgcctgaggg ctttcagcgc agcgagttcc tgctcgagca cggcggactg gacctcatcg
     4321 tcgaccggcg cgacatgcgc gatcggttgg cgcaacttct tcggctgctg cagaaacgac
     4381 cgccattgca agccgcttga gatcgctggc cgactggctg agctttcagc agcgagcgca
     4441 tccgcgcgcg gtggatctct ccctggaacg cgtctgctca gtcgccgcgc gactgggact
     4501 gctgcagcgt caggccctgc tggcgacggt tgcaggcaca aacggcaaag gctccgtcgc
     4561 caccacgctg gcgtgcttgc tgcaatcctg tggccagcgc gtcggcctat tcacctcgcc
     4621 gcatctggtg cgctacaacg aacgcatccg catcgacggc gaggctgcat ccgacagcga
     4681 tctggtcagc gccttcgagc acattgaatc cgcgcgcggt gaggtcacgc tgacgttctt
     4741 cgagtacaac gcgctggcgg cactggagct gtttcgccgc gcgcgggttg aggcgatggt
     4801 gctcgaagtg ggtctgggcg gccggttgga tgcgaccaac ctgctcagcg ccgatgtcgc
     4861 ggtcgtatgc tcgatcggct tcgatcatcg tgactggctg ggcaataccc tggagcagat
     4921 cggcgccgag aaggcaggca tttttcgccc gggccagaag gtggtgctcg gcagccccga
     4981 gatgccacag agcgtgtggc aggcagcgag cgcccttggt tgcgcggtgt ggaccgccga
     5041 acgcgagttc agctggtcgc tgcagccttc gcccgacgcg ccaccggcgt ggaactacca
     5101 cagcgcgcgc tgtgagctgt cagcgcttcc cgcaccggcg cttgcgggcc acattcaata
     5161 tcgaaacgcg gccagtgcgc tgaccgcgct gcagctgctc gatgtgccgc accgctgcga
     5221 gcgcagccgc gtgatcgcag gacttgaacg cgtcctgctg cccgggcgtt tccaaatcgt
     5281 ccccggcgag gtcgaatgga ttctggatgt ggcacacaac gaaccggcgg cacaggtgct
     5341 tgcacaatcg ctgcgcgaac gcggcgccgc gggcaggacc tgggcggtat tcggcacgct
     5401 caacgacaag gacagcgcag cgatcgcttt gacgctggac gcgcagatgg atcactgggt
     5461 gctggcgggc attgacgatc cgcgcggact gagcgcacag gcgctgcgtg agcgcttgtc
     5521 gtcgctgcgc gccgaggtgg aacttgcgga cgatgtgatc agtgcctgtg agcgcgtgcg
     5581 cgcacgtgcc cacgcggggg accgcgtggt ggtgctcgga tcatttcatg tggtcggtcc
     5641 ggcgctcgcg tggcttgggc tatactgaaa ccaacacagg tatggaaacc cggctgaaac
     5701 agcgactcac tggcgcggcg atcatagtcg cgctgatcgt ggcactggtt cctgaaatgt
     5761 ttcgcggtca ggccggcgcg ccgcccgcga acactgccag cagcagcagc ggtgagggac
     5821 caccggtacg cacctatacg atcgatctat ccaacaatcc gaagaacggt ccgctgcaat
     5881 cggccaatga agccccgccc gcatccgcac cggccgcgtc cggatcgagc tcgcccgcgg
     5941 caccggtgga gtcaccaccg gctccagccc cggctgccgc ggtggcagca cccagggccg
     6001 cgccctcatc gacgccgcca ccgtcgccgc caccgtcgca cggcagcgcg cctgcgccac
     6061 cccatgccac acgggcagcc ccgccggtga gctcgccggc agcggcgccg gccaggaccg
     6121 ccaccgcccc cacgggcaac tggagcgtac agctgggact gttctccaag cgtgaaaacg
     6181 ccgagcgcat gatcagcgag gccaagacca agggcttcaa tcccgcgatt gcgggccctg
     6241 acgccaaggg ccagtttcat gttcacgtaa cgggtttgac agatcgcgtc gcggcccagg
     6301 ctatggcgca gaaactgaaa ggcgaaggcc tgccggcggc ggtggttgcc ccgtagtgcg
     6361 aaccagcgcc tacagcttag gatgggttga tgtttagaat tcgcgcaccc actgggtcag
     6421 gtgtgtcagc atgaattggt tcgactattt gctgatcgtc gtgatggtgt tgtcgctcat
     6481 cgccgggctg atgcgtggcc tgctgcgcga ggcgatcggg ctgatcacat gggtggtcgc
     6541 aatctggatt gcatttcact acgccgcgca gcttgaaccc tacctcggcg gcctgcttgc
     6601 caatgattcg ctcagaccct ggatcgcacg ttcgctgatt tttctggtgg tgctgctgat
     6661 cggaaccacg ctggcggcgg tgatttcaca cttcatccgt gtgtcgatgt ttgccggcat
     6721 ggaccgcctg tggggtgcgc tgtttggcat cgtgcgcggc atggtggtga tcggagcgat
     6781 cctcatcctg tgccacggca cgcggctgca gacagaaacc tggtatcgcg cctcggtgct
     6841 ggcgccgtat ggtgagcatg tcgcgaatat cctgcgggct ttggtcgggg agcgtaaaat
     6901 cacagttgag ccttctgtca gcagcgccag ctagttatcg aggtactcaa ccccaacatg
     6961 tgcggcatcg tcggtatcgt cggtaccagt cccgtcaatc agcgcctcta tgacgcgcta
     7021 accgtacttc aacatcgagg tcaggacgcc gccggcatca tgaccgctca cgaggggcgg
     7081 ctgtgttcac gcaagggccc cggtttggtg cgcgacgtgt tcgagcagca tcacatgctg
     7141 gaactgcagg gcaacgtagg tatcgggcat gtgcgctacc cgaccgcagg ctgtgacagt
     7201 gcctcggaag cgcaaccctt ctacgtcaac gcgccgtatg gcatctgtct cgctcacaac
     7261 ggcaacctga ccaacgccgt tgaactggcc gaagtgatga ttcgcgagga tcggcgtcat
     7321 ctgaacaccg aatccgactc ggaagtgctg ctcaacatct ttgcctccga gctgcaacgc
     7381 gtcggcaccc cgcgtgtgac gccggctgac atctttgccg cggccagcgc ggtctatcga
     7441 cgctgccgcg gcggctacgc ggtggtggcg atggtgatcg ggcacggcat tctcgggttt
     7501 cgcgatccca acgggatccg gccgctggtg ctgggctcac gccgtaccgc gcggggtacc
     7561 gagtacatgc tggcctccga gagcgtggcg ctggccatgc tgtcgttcga actggtgcgc
     7621 gacgtcgcg
//
LOCUS       DATMRM010000141         4452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1006838, whole genome shotgun sequence.
ACCESSION   DATMRM010000141 DATMRM010000000
VERSION     DATMRM010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006838"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="VNZ06_04195"
     CDS             complement(<1..1446)
                     /locus_tag="VNZ06_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWW19983.1"
                     /translation="MLQSDASNSSPVGAAMAAARAQVQPVTSNSALDLSWRVVALANI
                     YRLLLPPMLLTLQWATRQSPSVGMNNPQLFVLICAVYWLLGALVAFGGRGHWPSRRVL
                     VVVNIVLDTAAICWLLYYSGGVSSGLGILLVIPVGAMSLLAEGRATLGVASMAALGLL
                     TQQILAVTIGHAQTNDYLLAGILGAVLFLVSLLAWPVSSRLRESEALVRRQEIDLANL
                     AQLSQYIVQHLRESILVVDPSDGIRLINESAAQVLGDTVALPGALLGEVAPRLLYLLE
                     TWRRDPGVPSVDAGTFVAADGARIIRPHFAPLGTSEPAPVIVFLEDTGEIAARVQQTK
                     LAALGRLSASIAHEIRNPIGAMSHAAQLLAESPELNGEDRRLTEIVQVNSTRVSTIID
                     NILQLSRREAPKPETVMLAAWTQRFRDEFNATLQLQGKGLVIDLAAEDIHVRIDGSQL
                     HQIVWNLCQNSVTHALPDASDSSIELRYGRLA"
     gene            1666..2133
                     /locus_tag="VNZ06_04200"
     CDS             1666..2133
                     /locus_tag="VNZ06_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19984.1"
                     /translation="MAVAPPPPTPMQIVVYPAKGQSAEQLDRDRYECHVWAVQQSGFD
                     PSQSGLPPGQRVVVEPANPPGSGTAIGAIAGAILGAAIAGPRQAGAGLVFGGITGAAV
                     GATSDANAQAQANYEQHRLDAGYNAAAAQNAQRASNYRRAISACLDARGYTTA"
     gene            2186..2905
                     /locus_tag="VNZ06_04205"
     CDS             2186..2905
                     /locus_tag="VNZ06_04205"
                     /inference="COORDINATES: protein motif:HMM:NF040452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HWW19985.1"
                     /translation="MNHATKHGTFLGIALLALGLALGASAGFADEHHPERSEHFERAP
                     PVRGQVFDNRYNHGHYYPGHGAVVHELPTGYHPYFFHGEHYYFAGGVWYHPGPFGFVV
                     VGPPLGLFVATLPAFYTTVWFGGVPYYYANEAYYQWDAGQNGYVVVNAPPGADQPGAP
                     PAGPAPAPADNVYIYPRNGQSSDQQAADRFECHDWAKNQTGFDPTQSGGGVPPAEINS
                     RREQYQRAMGACLEARGYSVK"
     gene            3020..4198
                     /gene="sucC"
                     /locus_tag="VNZ06_04210"
     CDS             3020..4198
                     /gene="sucC"
                     /locus_tag="VNZ06_04210"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HWW19986.1"
                     /translation="MNLHEYQSKKILAGYGVPVPKGRVADSADEAVEAAKALGGGVWV
                     VKAQVHAGGRGKAGGVKLARDLEQVRAAAAGMIGQRLVTKQTGAAGLPIERVYVECGS
                     EIARELYLSLTLNRERGRIAVVASAAGGMDIETVAHDTPEKILSATVHPAAGLQPYQC
                     RQLGFALDLKAKQLDQFQSLVTALYRLYLERDASLIEINPLIVTKGGELFALDAKLDI
                     EANALFRQKEMAALRDPGQEDPMEQRASEHDLNYVSLDGNIACMVNGAGLAMATMDLI
                     KLHGGQPANFLDVGGGATSERVTAAFQLILSNANVRAILVNIFGGIVRCDLIADGIIN
                     AVKNVGVTVPVVVRLEGTNAPAARELLARSGLAIIAAADLTDAARKVVTAAKSSKKAA
                     "
     gene            4213..>4452
                     /locus_tag="VNZ06_04215"
     CDS             4213..>4452
                     /locus_tag="VNZ06_04215"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Catalyzes the only substrate-level phosphorylation
                     in the TCA cycle; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HWW19987.1"
                     /translation="MSILVNKRTKVLCQGFTGKQGTFHSEQCLAYGTKLVGGVTPGRG
                     GDTHLGLPVFDTMRDAVASTGATASMIYVPAPYAAD"
BASE COUNT          830 a         1392 c         1457 g          773 t
ORIGIN      
        1 ggccagtctt ccatagcgca gctcgatgga gctgtcagag gcgtcaggca acgcgtgcgt
       61 cacgctgttc tggcacagat tccacacgat ttgatgcagc tggctgccat cgatgcgcac
      121 atggatgtct tcggctgcga gatcgatcac taagcctttg ccctgcaact gcagcgtggc
      181 attgaactcg tcgcgaaagc gctgcgtcca tgcggcgagc atgaccgtct ccggcttcgg
      241 cgcttcgcgt cgcgatagct gcaggatgtt gtcgatgatg gtgctgacgc gcgtggaatt
      301 gacctgcacg atctcagtca ggcggcggtc ctcgccattg agttcgggcg actccgccaa
      361 cagctgcgcg gcgtgactca tcgcgccgat cggattgcgg atctcgtggg caatgctggc
      421 cgacagacgg cccagcgccg caagcttggt ttgctgtacc cgcgcggcga tctcgccggt
      481 gtcctccagg aacacgatca cgggtgcggg ctcactggtc cccagcggcg cgaagtgcgg
      541 acggatgatg cgcgcaccgt cggccgcgac gaaggtaccg gcatcgacgc tcggcacgcc
      601 tggatcccga cgccaggtct cgagcagata gagcaggcgc ggtgccactt ccccgagcag
      661 tgctcccggc agtgcgaccg tgtcgccgag cacctgtgct gcggattcgt taatgagacg
      721 gatgccgtcg gacggatcca cgaccaggat gctctcgcgc agatgctgca cgatgtattg
      781 cgacagttgc gccaggttcg caagatcgat ttcctgccgg cgcaccagcg cttcgctctc
      841 ccgcaggcgg ctggataccg gccacgcgag cagcgatacc aggaacagca cggcgccgag
      901 gattccggcc aacaggtagt cgttcgtctg cgcgtggccg atggtcaccg ccaggatctg
      961 ctgcgtcagc agaccgagcg ccgccatcga tgcgacgccg agcgtcgcac gtccctccgc
     1021 cagcagtgac atggcaccca ccggtatcac cagcaggatc ccaaggccgc tggagactcc
     1081 accgctgtaa tagagaagcc agcagatcgc ggccgtgtcc agcacgatat tgacgaccac
     1141 cagcacacgg cggctaggcc agtggccacg tccgccgaag gccaccaatg cgcccagcag
     1201 ccagtagacc gcgcagatca gcacgaacaa ctgtggattg ttcatgccga cgctcggtga
     1261 ctgacgtgtt gcccactgca gcgtcagcaa catcggcggc agcagcagtc ggtaaatgtt
     1321 cgcgagcgcg acgacgcgcc aggacagatc gagcgcggag ttggaagtga ctggctgcac
     1381 ctgggcgcgt gcggcagcca tcgccgcacc taccggggag gagttcgatg cgtcggattg
     1441 caacatgctg cagcgcagta agctattgaa actgtccctg aaacgcgcac tctaagcatt
     1501 caggtcgggt taacaaggga gctgtcaacc ttttggaaaa taggtcgttt catccagcat
     1561 atgattacac catacatgtt gaatcaatta cttaaaccac gtacctggat ggcagtggcc
     1621 ctgggcgggg tcctcgcagc ctgcgcgacc cctccgccgc ggcgcgtggc agtggcaccc
     1681 ccgccgccca ccccgatgca gatcgtggtc tatccggcga aggggcaaag cgctgagcag
     1741 ctcgaccgtg accgctacga atgccatgtg tgggccgtgc agcagtccgg ttttgatccg
     1801 agccagtcag gcctgccgcc aggccagcgc gtcgtggtcg agcccgcgaa cccgccgggc
     1861 agtggaacgg cgatcggcgc gatcgccggt gccattctgg gggctgcgat tgccggtccg
     1921 agacaggccg gagcaggact ggtgttcggt ggtatcaccg gtgctgcggt gggagccacc
     1981 agcgatgcga acgcacaggc ccaggcgaac tatgagcaac atcgactgga tgccggctac
     2041 aacgcggctg ccgcgcagaa tgcgcagcgc gcctcgaact atcgacgtgc catcagtgcc
     2101 tgtctggatg cccgcggcta cacgaccgct tgatttcgcg cacccatttt ccgaggatcg
     2161 caagatcgaa aggacgatcg aatctatgaa ccacgcaacg aaacacggca ctttcctggg
     2221 cattgcgctg ctcgcgctgg gtctggcgct gggcgcctcc gccggcttcg ccgacgagca
     2281 tcaccccgaa cgctctgagc acttcgagcg cgccccgccg gtacgtgggc aggtgttcga
     2341 caaccgttac aaccatgggc actactaccc gggacacggt gcggtggtgc acgagttgcc
     2401 gactggctat cacccgtatt tctttcacgg tgagcactac tattttgccg gcggcgtctg
     2461 gtatcacccg ggcccattcg gcttcgtcgt ggttggcccg ccactgggtc tgttcgtggc
     2521 gaccttgccc gcgttctata ccaccgtgtg gtttggcggc gtgccgtatt actacgccaa
     2581 cgaagcctat taccagtggg atgcggggca gaacggctat gtggtcgtga acgcgccgcc
     2641 gggtgcggat cagccgggag caccgccagc cggtccggcg ccggcgcccg ccgacaatgt
     2701 ctacatctat cccaggaacg gtcagagctc cgatcagcag gccgccgatc gcttcgagtg
     2761 ccatgactgg gcgaagaatc agaccggttt cgatcccaca cagtccggcg gcggcgttcc
     2821 tcccgctgag atcaactcaa ggcgcgagca gtaccagcgc gcgatgggtg cttgcctcga
     2881 ggcgcgcggc tacagcgtga agtaagcggc aacgcccgca atccctgcgg tgtcgccagc
     2941 gcgcgccggg cggacgctgg cggcgggctc tgctttccgc gacaatcccc agcccttttg
     3001 gacgatcgga ttgccgcgga tgaacctgca cgaataccaa tcgaaaaaaa tccttgccgg
     3061 ctatggcgta ccggttccga aagggcgcgt ggccgacagc gcggatgagg cggtagaagc
     3121 tgccaaggcg ctcggcggcg gcgtctgggt cgtcaaggca caggtgcatg ccggtgggcg
     3181 tggcaaagcc ggcggcgtga aactggcgcg cgatctggaa caggtgcgcg cggcggcggc
     3241 cggcatgatc ggccaacggc tggtgaccaa gcagaccggt gctgcgggtc tgccgatcga
     3301 acgggtctat gtggagtgcg gctcggagat cgcgcgcgag ctgtacctga gtctgacgct
     3361 caatcgggag cgcggacgca tcgcggtcgt ggcgtcggcc gcaggtggaa tggacattga
     3421 gacggtggcg cacgacacgc cggagaagat tctgtcggcg accgtgcatc cggcggccgg
     3481 actgcaaccc taccaatgtc gtcaactggg cttcgcgctc gacctcaagg cgaaacagct
     3541 tgatcagttc cagtcgctgg taacggcact ctaccggctg tacctcgaac gcgacgcgag
     3601 cctgatcgag atcaatccgc tgatcgtgac caagggtggc gagttattcg cgctcgatgc
     3661 caagctggac atcgaggcga acgcgctgtt ccggcaaaag gagatggcgg cactgcgcga
     3721 tccgggtcag gaggatccga tggaacagcg cgccagcgag cacgatctca actacgtctc
     3781 gctcgacggc aacattgcct gcatggtcaa tggcgccggg ctggcgatgg cgaccatgga
     3841 tctgatcaaa ctgcacggcg gccagccggc gaattttctg gacgtcggtg gcggggcgac
     3901 cagcgagcgc gtgactgcgg cgtttcagct gatcctgtcc aacgccaacg tgcgtgcgat
     3961 tctggtgaat atcttcggcg gcatcgtgcg atgcgatctg atcgccgatg gcatcatcaa
     4021 cgcggtcaag aacgttggtg tgactgtgcc ggtggtggtg aggctggagg gtaccaatgc
     4081 gccggcggct cgcgagctgc tcgccaggag cggtctggca atcatcgccg cggcggatct
     4141 gaccgatgcc gccagaaagg tcgtgaccgc cgccaagtcc agcaagaaag ccgcttaagt
     4201 cgaagggacc gtatgagcat tctcgtcaac aagcgcacca aggtgctgtg tcagggcttc
     4261 acaggcaagc agggcacctt tcattccgag caatgcctcg cctacggcac caagctggtc
     4321 ggcggcgtga ctccaggccg tggcggcgat acgcatctgg ggctgccggt gttcgacacc
     4381 atgcgcgacg cggtcgcctc gaccggggcc actgcgagca tgatttacgt tccggccccc
     4441 tacgccgccg at
//
LOCUS       DATMRM010000142         3349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3786952, whole genome shotgun sequence.
ACCESSION   DATMRM010000142 DATMRM010000000
VERSION     DATMRM010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3786952"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VNZ06_04220"
     CDS             complement(<1..1134)
                     /locus_tag="VNZ06_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWW19988.1"
                     /translation="MNSNEQPDRHGLRRELGFRDLVPMQVLMCVGLTWAGTAAREGSG
                     HVMLWLAGTLLLFLPVAAVVGYCVKLWPLEGGVYQWTRHALGPFAGFLSAWSLFVWSI
                     TVVSTLGITIAAAIGYSMGPSGAWVAENNWVIAGLNLVLFAAILLLNVPGLGIARWVA
                     HLGTAVTLLVTALLLLLILYHPHASAAHPHHAPQRPFSLAFPAVTLLTLNLFSKLSFY
                     GLSGLEQVAVFAGETRDAARSVLRSAWIAAPLIAAIYICMSGSMLSYTPADQIDLTAP
                     IPQVLAAGFSAGTASTLDLGLYLGRAAIALLAIATFAQYAVIVAETSRLPMVAAWDHL
                     LPAWFMRLHPRYRTPTRSLLTFVAVALVFSLLASVGAGAQEAFQ"
     gene            complement(1131..>3349)
                     /locus_tag="VNZ06_04225"
     CDS             complement(1131..>3349)
                     /locus_tag="VNZ06_04225"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWW19989.1"
                     /translation="IEQEVAQDLSNPAYTLYAKLRRIMFAGTPYEHDALGTRPSFEKT
                     TAAALKSFHDTWYAPNNAILIVVGDLPLEPTMRTVRSLFSDIKSKSLPARPTWKLQPV
                     KAQHFRVDTDRPEGTQMIAMRTPGLDSPDFPALEVLSDVLSSHRFPLYDLVADGKALD
                     AEFVLDPLPHAGLAYATVSFPAGSDPAALENQVRAILQRVVTDGVPSELVAAAKLQER
                     SETEFQKNSIAGAASVWSEAVALYGLNSPQEDLSRIEKVTVADVNRVARQYLDLDHAV
                     SAVMIPHGSGQPKASGGSFGGPENIALGEAKPTPLPDWAQAALTRLTLPDLSTHPIVT
                     TLPNGLTLIVQPEEVSDTVSIYGHIRNRPEIQAPPGQQGIAQLLDAMLPYGTEQLDRV
                     AFEQALDAIGARENAGTDFSIQTLSANFERAAELLAANELHPALPADALEALRPQFSR
                     LVAARNHSPAHLAQLGVRRALFPASDPTLQEATPQSVASIKLDDLRNYRRSTFRPDLT
                     SIVVIGKVSPQQALATISRYFGAWSATGPTPAIDLPAVPPNRGSQLAVPDDSRVQDEV
                     LLAQTTALTRRDPDYYAFELGSAVLGGGFYSTRLSIDLRKNAGLVYSVESELQAGRTR
                     SVYFVNYACDPQNVSKAQQLVVRDLRAMQSQPVGEDELTRVKALLLRQIPLDESSIDE
                     IAHGFLQRRDDSLPLDEPTLAARRYIALKPAEVQDAFSKWVRPDDLVRVTQGPVAR"
BASE COUNT          598 a         1030 c         1146 g          575 t
ORIGIN      
        1 ctgaaaggct tcctgcgccc cggcgccgac cgatgccagc agactgaaga ccagggcgac
       61 cgcgacgaat gtcagcagcg agcgggtcgg ggtgcggtag cgcggatgca gacgcatgaa
      121 ccaggcgggc aacagatgat cccacgccgc caccatcggc aggcggctgg tttcggccac
      181 gatgaccgcg tactgagcga aggtggcgat cgccaagagc gcaatggcgg cgcgacccag
      241 gtacagaccc agatccagtg tgctagccgt gccggcactg aagcctgcgg ccagcacttg
      301 tgggatcggg gcggtcaggt cgatctgatc ggccggcgtg tacgacagca ttgaacctga
      361 catgcagatg tagatcgccg cgatcagggg tgcggcaatc caggccgagc gcagcacgct
      421 gcgcgccgca tcgcgcgtct caccggcaaa cacggcgacc tgctcgaggc cactgaggcc
      481 gtagaatgac agtttgctga agagattcag cgtcagcagc gtcaccgccg ggaaggccag
      541 tgagaacggc ctttgtggcg catgatgcgg atgggccgcc gaggcgtgcg gatgatagag
      601 gatcaatagc agcagcagcg ccgtcaccag caacgtgacc gccgtgccca aatgcgccac
      661 ccagcgggcg atgcccagtc ccgggacatt gagcagcaga atggccgcga acagcacgag
      721 attgaggccg gcgatgaccc agttgttttc ggcgacccag gcgccgctgg gacccatcga
      781 ataaccgatc gcggccgcga tggtgatgcc taaggtggat accacggtga tcgaccagac
      841 gaagaggctc caggcgctca ggaaaccggc gaacggtccg agtgcatgcc gcgtccactg
      901 atagacgccg ccttcgagag gccagagctt gacgcagtag cccaccactg ccgcgaccgg
      961 caggaacagc aacagcgtgc cggccagcca cagcatgacg tggcccgagc cttcacgcgc
     1021 ggcagtgcca gcccaggtca ggccgacgca catcagcacc tgcatcggca ccagatcgcg
     1081 gaatccgagt tctctgcgca gcccgtggcg gtcgggttgc tcgttgctgt tcatcgcgcg
     1141 accggcccct gcgtcacgcg caccagatca tcggggcgca cccacttgct gaatgcgtcc
     1201 tgcacctcgg ccggcttcag cgcgatgtag cgtcgcgccg ccaacgtcgg ttcatccagc
     1261 ggcaggctgt cgtcgcgccg ctgcaggaag ccgtgcgcga tttcatcgat gctggattca
     1321 tcgagtggga tctgccgcag cagcagcgcc ttgacgcgtg tcagctcgtc ttcgcccacc
     1381 ggttgggact gcatcgccct caggtcgcgc accacgagct gctgtgcctt gctgacattc
     1441 tgtggatcgc aggcgtaatt cacgaaatag acgctgcggg tgcggccggc ctgaagttcc
     1501 gactcgacgc tgtagaccaa tcccgcgttc ttgcgcaggt cgatgctcag acgggtcgaa
     1561 tagaagccgc caccgagtac cgcgctgccc agctcgaatg cgtagtagtc cgggtcgcgg
     1621 cgcgtcaagg cggtggtctg cgccagcagt acttcatcct gcacgcggct gtcatcgggc
     1681 accgcgagct gcgagccccg gttcggcgga accgccggca gatcgatcgc cggtgtcgga
     1741 ccggtcgcgc tccaggcgcc aaagtatcgg ctgatggttg ccagcgcctg ttgcgggctg
     1801 accttgccga tcacgacgat cgatgtcaga tccggacgaa aggtgctgcg ccggtagttg
     1861 cgcaggtcgt cgagtttgat cgaggcgacg ctctgtgggg tggcttcctg cagggtggga
     1921 tcgctcgccg ggaacagcgc gcgacgtacg ccgagctgcg ccaggtgggc cggactgtga
     1981 ttgcgggctg cgaccagccg cgagaattgt gggcgcaggg cttcgagtgc atccgcagga
     2041 agggccggat gcagctcgtt ggcggccagc agctccgcgg cgcgctcgaa gttggcactg
     2101 agggtctgaa ttgaaaagtc agtgccggca ttttcgcggg cgccgatggc atcgagcgct
     2161 tgttcgaacg cgacgcgatc gagctgttcg gtgccatagg gcaacatcgc atccagcagc
     2221 tgcgcgatac cctgctgtcc gggcggcgcc tggatctcgg ggcgattgcg aatgtgtcca
     2281 tagatgctga ccgtgtcgct gacctcctcg ggctgcacga tcagggtcag gccattcggc
     2341 agcgtcgtga cgatggggtg cgtgctgaga tccggcaggg tcaggcgcgt gagtgccgcc
     2401 tgtgcccagt cgggcaacgg ggtgggtttg gcttcgccga gcgcgatgtt ctccggtccg
     2461 ccgaaactgc cgccggaggc tttgggttgt ccggagccat gcgggatcat cacggcactg
     2521 accgcatgat cgaggtccag gtattgacgt gccacgcgat tgacgtccgc gaccgtgact
     2581 ttctcgatgc ggctgaggtc ctcttgcggt gagttcaggc catagagcgc caccgcctcg
     2641 gaccataccg aggcggcgcc cgcgatcgag ttcttctgga actcggtttc gctgcgttcc
     2701 tgcagcttgg cggccgcgac cagttcgctc ggcacaccgt cggtgacgac gcgctgcagg
     2761 atcgcgcgca cctggttttc cagcgccgca gggtcgctgc cagcgggaaa cgagacggtc
     2821 gcataggcga gcccggcgtg cggcagcgga tcgagcacaa actcggcatc gagggctttg
     2881 ccgtcagcga ccagatcata gagcgggaag cgatgactgc tcagcacatc gctcaacacc
     2941 tcgagcgccg gaaagtccgg actgtccagg cccggtgtgc gcatggcaat catctgagtg
     3001 ccttccgggc ggtcggtgtc gacgcggaag tgctgggctt tgaccggctg cagcttccag
     3061 gtaggacgcg ccggcaagct cttcgatttg atgtcggaga acagtgagcg cacggtgcgc
     3121 atcgtgggtt cgagcggtag atcaccgacc acgatcagaa tggcattgtt cggtgcgtac
     3181 caggtatcgt gaaagctctt cagcgcagcg gccgtggtct tctcgaagga gggccgtgtg
     3241 ccaagcgcat cgtgttcgta cggagtacct gcgaacatga tccggcgcag cttcgcgtac
     3301 agcgtgtagg cgggattgga cagatcctgc gcgacctcct gttcgatcg
//
LOCUS       DATMRM010000143         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_399069, whole genome shotgun sequence.
ACCESSION   DATMRM010000143 DATMRM010000000
VERSION     DATMRM010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399069"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1688
                     /locus_tag="VNZ06_04230"
     CDS             <1..1688
                     /locus_tag="VNZ06_04230"
                     /inference="COORDINATES: protein motif:HMM:NF017826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HWW19990.1"
                     /translation="LSGSGKDERWDTLILGDVHTPLVQELPGGGHEIIFTYVLGPKEY
                     VLAVRDYTVGSPVNLQPLLAKDGITCTRHEADGLGGLAVAWINKLRSAGQVHDQLIAR
                     PFGWNFLNGACTGLAIGGVLYRPGGMVERVACDDKKLAATYRPMGELAVWRRAAHLFE
                     GKRPDLQALIACAFGAPLISLTGDVRGMTLNFWSAGSGIGKSTAIKIGQSVWGNYTAM
                     QSMRDTPNSVMRSLAGPRVLIRYWDEFRITRDRQGEFVELVYTLPQGKERMRLMADIT
                     IRETGEWDTMLAMTSNKSHADVLLLDNDGTDSGLLRLLEIEMDKQMMPFDAAAGPLIK
                     ATETNYGHAGRIYAAYLGAQVDAVRLQLAAVSETLGRDLNLQQDERYYAAAIATIVVG
                     ATIACHLDLFAFDVHAIYDVLKRAVIASRSARQVRTVVTGAGRYELGPIMAQFINAMG
                     DHLIRTDVLATAGSGKVTPIVTPKSSSIIKAQIAEKAGMIRLHREEFFRWLAVRNLPS
                     APVLDQMGQDWNARQKRAALAGGTSYATGQLWVVDVPRGDPELEAYFDTGGRP"
     gene            complement(1818..>2259)
                     /locus_tag="VNZ06_04235"
     CDS             complement(1818..>2259)
                     /locus_tag="VNZ06_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW19991.1"
                     /translation="SQQGVTDSTGKTILPPEAIGTGGVAAQALGFQPSVVTEFREGRN
                     AALEAREEAQSARSSLVNRYLAATPIDRHAIQSEINQFNADPMHRGMAITVDQLLRDE
                     ASRRKQAREPFGLKLPAKAARTLGQAGAFANVPQSAQIGAAAPP"
BASE COUNT          375 a          742 c          780 g          362 t
ORIGIN      
        1 tgctgtcggg ttcggggaag gacgagcgct gggacacgct gatcctgggc gatgtgcaca
       61 cgcccttggt gcaggagctg ccgggcggcg gccacgaaat catcttcacc tacgtgctgg
      121 gccccaagga gtacgtgctg gcggtgcgcg actacaccgt cggcagcccg gtgaacctgc
      181 aacctctgct cgccaaggat ggcatcacct gcacccgcca cgaggcggac ggattgggag
      241 ggctcgcggt ggcctggatc aacaagctgc gttcggcggg acaggtgcac gaccagctga
      301 tcgcccggcc gttcggctgg aactttctga acggcgcctg caccgggctg gcgatcggcg
      361 gtgtgctgta tcgccccggc ggcatggtgg agcgcgtcgc ctgcgatgat aaaaaactgg
      421 ccgcgaccta ccgcccgatg ggcgagctcg cggtctggcg ccgggcggcg catctgttcg
      481 agggcaagcg gcccgatctg caggcgctga tcgcctgcgc gttcggcgcg ccgttgatct
      541 cgctcaccgg cgacgtgcgc ggcatgaccc tgaatttctg gtcagccggg tccggcatcg
      601 gcaagagcac cgcgatcaag atcggccaga gcgtgtgggg caactacacc gcgatgcaga
      661 gcatgcgcga tacgcccaac agtgtgatgc gctcgctcgc gggacccagg gttttgatcc
      721 gctactggga cgaattccgg atcacccgcg accgccaggg cgagttcgtc gagcttgtct
      781 atacactgcc gcagggcaag gaacgcatgc ggctgatggc ggacatcacc atccgcgaga
      841 ccggcgagtg ggacaccatg ctggcgatga cttccaataa gtcccacgcc gacgtgctct
      901 tgctggacaa cgacggcacc gattccgggc tgctcaggtt gctcgagatc gagatggaca
      961 agcagatgat gccgttcgac gcggccgccg ggccgctgat caaggccacc gagaccaatt
     1021 acggccacgc cggaagaata tacgccgcct atctcggcgc ccaggtggac gcggtgcgcc
     1081 tgcagctggc cgccgtgagc gagaccctgg gcagagacct gaacctgcag caggacgagc
     1141 gctactacgc cgcggcgatc gcgacgatcg tggtgggcgc caccatcgcg tgccacctgg
     1201 acctgttcgc cttcgacgtg cacgcgatct atgacgtgct gaagcgcgcg gtgatcgcct
     1261 cgcgcagcgc ccgccaggtg cgcaccgtgg tcaccggcgc cgggcgctac gagctcgggc
     1321 cgatcatggc gcagttcatc aacgcgatgg gggatcacct gatccgcacc gacgtgctgg
     1381 ccaccgccgg cagcggcaag gtcaccccga tcgtcacgcc caagtcctcc agcatcatca
     1441 aggcgcagat cgccgagaag gccgggatga tccgcttaca cagagaggag ttctttcgct
     1501 ggctggcggt gcgcaacctg ccctcggcgc cggtgctcga tcagatgggc caggactgga
     1561 acgcgcgcca gaagcgcgcc gcgctggccg gcggcaccag ctacgccacc gggcagctct
     1621 gggtggtgga cgtgccccgg ggcgacccgg agctcgaagc ctatttcgac accgggggca
     1681 gaccatgaac gacatgatgt ttacgccggg cgtaaacttc cggctggacg ccgaacgcaa
     1741 gcagcgggct tatgccgaac aacgcaggat ccataccgac acgcagttgc gcgcgttcat
     1801 ggcctggcag gccgaggtca cggcggtgcg gcggcgccta tttgggctga ttgcggcacg
     1861 ttcgcgaacg cgcccgcctg ccccagagtg cgtgccgctt tcgctggcag cttgagcccg
     1921 aacggttccc gcgcctgctt gcgccggctc gcctcgtcgc gcagcagctg gtcgacggtg
     1981 atcgccatgc cgcgatgcat gggatcggcg ttgaactgat tgatctcgct ctggatcgcg
     2041 tgacggtcga tcggcgtggc ggccaggtag cggttcacca gggaactccg cgcgctctgg
     2101 gcttcctcgc gcgcctcgag cgccgcgttg cgcccctcgc ggaactcggt gacgacgctc
     2161 ggctggaagc ccagcgcctg ggcggcgacg ccaccggtgc cgatcgcctc aggcggaagg
     2221 atggttttgc cggtgctgtc ggtgacgccc tgctgcgag
//
LOCUS       DATMRM010000144         6752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2860547, whole genome shotgun sequence.
ACCESSION   DATMRM010000144 DATMRM010000000
VERSION     DATMRM010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2860547"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VNZ06_04240"
     CDS             complement(<1..197)
                     /locus_tag="VNZ06_04240"
                     /inference="COORDINATES: protein motif:HMM:NF023393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HWW19992.1"
                     /translation="MSRSANRVQLARAFVLHQRPFRDSSLIVEVFARDHGRLTVFAHA
                     ARRSRSRFAALQPFSALLMSW"
     gene            complement(194..1084)
                     /gene="era"
                     /locus_tag="VNZ06_04245"
     CDS             complement(194..1084)
                     /gene="era"
                     /locus_tag="VNZ06_04245"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HWW19993.1"
                     /translation="MTDFRSGFVALAGRPNVGKSTLLNALVGHKLSIVTPRPQTTRHR
                     VIGLLHLPQAQIAFVDTPGLHHGPKRALNRSMNRTAAAAVSDADLVLFVVEALKWTDE
                     DELALSRIRQTEHAVVAVVNKVDRVRPRARLLPYLAELAARHTFREIVPVSASKTDNL
                     DDLQRVIVRQLPQAQAMFPADQLTDRSTNFRIAEIIREKLTLELVEELPYGVAVEIEQ
                     IQTEEDGRKSVSAVIWVDRPGQKPIVIGAEGARLKRVGRAARMEINHLFGERFHLILW
                     VKVREDWADDARALRELGME"
     gene            complement(1081..1776)
                     /gene="rnc"
                     /locus_tag="VNZ06_04250"
     CDS             complement(1081..1776)
                     /gene="rnc"
                     /locus_tag="VNZ06_04250"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013344247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HWW19994.1"
                     /translation="MPQERAEGLRRWLRDALGYEAVDLTLFETALTHRSADGPNNERL
                     EFLGDAVLGLVCAQYLYERFGQADEGALSRLRASVVNGESLAQLAAGLSLGEQLSLGP
                     GELKTGGFRRESILADALEALCGALYLDAGLPRAGAMIEQLLAPALQGMSLPADLKDP
                     KTRLQELLQGRGLPLPRYSVDNVEGELHAQVFRVTCEVEPLALRAGATGSSRRRAEQV
                     AAARILEQIQADS"
     gene            complement(1792..2154)
                     /locus_tag="VNZ06_04255"
     CDS             complement(1792..2154)
                     /locus_tag="VNZ06_04255"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HWW19995.1"
                     /translation="MRHSQRGVTFIGWILLLAPIAILIFVAIRLTPIYLNYMSVVKAL
                     NTVASENKGETSSAEGLRNALAKHFQVGYVEKPDPRDLDIHRDGTHWAVIADYEETAP
                     LFGNLSLLVEFHKEVDVQ"
     gene            complement(2176..3252)
                     /gene="lepB"
                     /locus_tag="VNZ06_04260"
     CDS             complement(2176..3252)
                     /gene="lepB"
                     /locus_tag="VNZ06_04260"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWW19996.1"
                     /translation="MLTQLLTILTIVAVVTGVIAVVEDWFLRPSRAPGADASKSSFMS
                     AVHFLLPIGCVGAVLRLLMSERLDFSLILVIVVAATGLVWLLDSLLLAPMRARAAQAR
                     GVDAASVPLPVTVDGARSFFPVALIVLVVRAFVFEPFRIPSDSMMPTLLDGDFIIVNK
                     FAYGLRLPVLDTKVVPIGEPKRGDVVVFHWPPDPAINYIKRLVGLPGDRVRIKSDQLI
                     VNDIPIPLTDLPRYDDGCYHNMRQSTEILGTHKHQILWCLTPDDLAAAPTPSCNRHIG
                     QNYVCRETNPPGDFDKGDADEMLVPPGEYLMIGDNRDNSSDGRYWGFVPEDHLVGKAT
                     RIWFNWDLGRSGGPQWSRMFHRIE"
     gene            complement(3264..5057)
                     /gene="lepA"
                     /locus_tag="VNZ06_04265"
     CDS             complement(3264..5057)
                     /gene="lepA"
                     /locus_tag="VNZ06_04265"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HWW19997.1"
                     /translation="MQRIRNFSIIAHVDHGKSTLADRLIQLCGGLEAREMQEQVLDSM
                     DLERERGITIKAQSVTLSYQAHDGQPYQLNLIDTPGHVDFSYEVSRSLAACEGALLVV
                     DASQGVEAQSVANCYTAHEQGLEVLPVINKIDLPSADPDRVIKEIEEIIGIEAHDAVR
                     VSAKTGEGVPELLEAIIHRIPAPKGDTEAPVQALIVDSWYDNYVGVVSLVRIVNGVLR
                     TGDKIRIMSTGRAHNIDKLGRFTPKSVASETLSAGEVGFVIAGIREIDGAPVGDTITL
                     EARPAVEPLPGFKQIQPRVFAGLFPVNAEDYESFRDALAKLKLNDSALHYEPEVSTAL
                     GFGFRCGFLGLLHMDIVQERLEREYNIDLVTSAPTVIYEVARTNGTLEYVDNPSKLPP
                     ANEVAEVREPIITASILVPPDYVGGVLGLCSEKRGVQKKMLYLGSQVSLQYELPLAEV
                     VLDFFDRLKSATRGYASFDYVFSHFQVAPLVKLDILINGDPVDALSIIVHREHAYERG
                     RDLVDTMQELIPRQMFEVAVQAAIGTHVIARSTVKALRKNVLAKCYGGDVSRKRKLLE
                     KQKEGKKRMKQVGSVEIPQEAFLAVLRVGRK"
     gene            complement(5110..5337)
                     /locus_tag="VNZ06_04270"
     CDS             complement(5110..5337)
                     /locus_tag="VNZ06_04270"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HWW19998.1"
                     /translation="MLLMRPECGLCESMLAQLEALRAEYPLPPIQVVNVDSDPELQRR
                     YGLKIPVLMMERVPICAVRLDRQELLRALRT"
     gene            complement(5346..6605)
                     /locus_tag="VNZ06_04275"
     CDS             complement(5346..6605)
                     /locus_tag="VNZ06_04275"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HWW19999.1"
                     /translation="MRRKRRVPRVASQATGAATLWAAGGCAALLLSGIAQAADDDPRY
                     WLARTDQALSKLNYEGVFVHEHGGDTETLRVIHRVGSDGVSERLLSMDGSGREFIRRG
                     TQLTCYLPDKRVVLVEKSSDSGLLLASLPRLEAGAAGQYQITELTRTRASGRPVRVIA
                     ITPMDELRYGYRVWVDEASAMPLKTQLRSRSGEVLEQLVFTDLRLQRHISERELQPAV
                     DTRGFTFVHQDNDSTDTSPLSVSWQAAMLPPGFTMTTSARQVLPGGPVEHLVFTDGMA
                     SVSVFVEVGRDPGTAQRDDAARLGTSSAYSTTVQGYRVTAVGEVPPDTVRAIALSIRT
                     ATNLPSPVYTYEAIELPLALALRSMDTGSIFDQTPASAQPEGSFEHRGMLNMPRSNPV
                     GAPASAFGASPPMTAFGPSSNPVPPRH"
     gene            complement(6571..>6752)
                     /locus_tag="VNZ06_04280"
     CDS             complement(6571..>6752)
                     /locus_tag="VNZ06_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20000.1"
                     /translation="ADFIVAHSEYSTPLMRRSLLSALVSSEEIAEPAAANAAQAGSAR
                     AGQGNAAQATGTTGR"
BASE COUNT         1228 a         2244 c         2085 g         1195 t
ORIGIN      
        1 ctccaggaca tgagcagcgc agaaaagggc tgcagggcgg cgaaacgcga gcgcgagcgc
       61 cgggcggcgt gcgcgaatac tgtcaggcgc ccgtgatcgc gcgcgaacac ctcgacgatg
      121 aggctgctgt cgcgaaacgg ccgctgatgc agcacgaaag cgcgtgccag ctgtacccga
      181 ttggcggagc ggctcattcc attcccagct cgcgcagagc acgcgcgtca tcggcccagt
      241 cctcgcgtac cttcacccac aggatcagat gaaagcgctc gccgaacagg tggttgatct
      301 ccatgcgcgc ggcacgtccc acgcgcttga gtcgcgctcc ctcggcgccg atcacgatcg
      361 gcttctgccc ggggcggtcc acccagatca ccgcggagac actcttgcgg ccgtcctctt
      421 cggtctggat ctgctcgatt tccaccgcca caccgtaggg cagttcctcg accagctcga
      481 gcgtcagctt ctcacggata atctcggcga tgcgaaaatt cgtcgagcga tcggtgagct
      541 gatccgcggg gaacatcgcc tgcgcttgcg gcagctgccg cacgatcacg cgctgcagat
      601 catcgaggtt gtcggtcttg gaggccgaca ccggcacgat ctcccgaaag gtgtgtcgcg
      661 ccgcgagctc cgccagatag ggcagcagcc gtgcgcgcgg tcgaacgcga tcgaccttgt
      721 tcacgacggc caccaccgcg tgctcggtct gacggatgcg tgacagcgcc agctcgtcct
      781 cgtcggtcca cttgagcgcc tcgaccacga acagcaccag atcagcatca ctgaccgctg
      841 cggcggcagt tcggttcatc gagcgattga gcgcgcgttt gggaccgtga tgcaggccgg
      901 gtgtatcgac aaatgcgatc tgtgcctgcg gcaaatgcag cagtccgatc acgcgatgac
      961 gggtcgtttg aggtcgcggc gtcacgatgc tgagcttgtg cccgaccagc gcattgagca
     1021 gcgtcgactt gccaacgttg gggcgacccg cgagtgcgac aaagccgctt cggaaatctg
     1081 tcatgagtcg gcttgtatct gctccagtat gcgtgccgcg gccacctgtt ccgcgcgccg
     1141 acggctcgat ccggtcgcac ccgcgcgcag ggccaagggt tcaacctcgc aggttacgcg
     1201 aaacacctgc gcgtgcaatt ctccttcgac gttatccacg ctgtagcgtg gcagcggcaa
     1261 cccacgcccc tgcagcagct cctgcagccg cgtcttcgga tccttcagat ccgccggcag
     1321 gctcatgccc tgcagtgccg gggccagcaa ttgctcgatc atcgcccccg cccggggcag
     1381 tccggcatcc aggtagagcg cgccgcacag cgcctccagg gcatcagcca ggatcgattc
     1441 gcgccgaaag ccgccggtct tcagttcccc tgggcccagc gacagctgct cgccgagcga
     1501 caggccggcg gcgagctgcg ccagtgactc gccgttgacg acgctggccc gcagtcggct
     1561 cagcgcgccc tcgtcagcct ggccgaatcg ctcgtacagg tattgcgcgc acaccagtcc
     1621 gagcacggcg tcgccgagaa actcgagccg ttcgttgttg gggccgtccg cgctgcgatg
     1681 cgtcaacgcg gtctcaaaca gcgtcagatc gaccgcctcg tacccgagcg cgtcgcgtag
     1741 ccagcgacgc agaccctcag cgcgctcttg cggcataaca ggctgcgtcg actattggac
     1801 atcgacctct ttgtgaaact cgaccagcag cgagagattg ccgaacaggg gcgcggtttc
     1861 ctcatagtcg gcaatcaccg cccagtgcgt gccgtcgcgg tgaatatcga gatcccgagg
     1921 atcgggtttc tcgacgtagc ccacctggaa gtgcttggcg agcgcattgc gaagaccctc
     1981 ggcgctgctg gtctcgcctt tgttctccga ggcgacggta ttgagcgcct tgacgacact
     2041 catgtagttc aggtagatcg gggtcaggcg gatggcgaca aagatcagaa tcgcaatcgg
     2101 tgcgagcagc agaatccagc cgatgaaggt cacgccgcgc tgtgaatgac gcatatgcaa
     2161 gactccctgt aaggatcact cgatgcggtg gaacatacga ctccactgag gtccgcccga
     2221 gcgtcccaga tcccaattga accagattcg cgtggctttg ccgaccaagt ggtcctcagg
     2281 aacgaagccc cagtaccggc catcggagct gttgtcccga ttatcgccga tcatcaggta
     2341 ttccccaggg ggtaccagca tctcgtcggc atcacccttg tcgaagtccc ccggaggatt
     2401 ggtttcccgg cagacataat tctgcccgat atgccgattg cagctcgggg taggcgccgc
     2461 cgccagatca tccggcgtca ggcaccatag gatctggtgc ttgtgggtgc cgaggatctc
     2521 ggtggattgg cgcatgttgt gatagcagcc gtcgtcatag cgcggcaggt cggtcagcgg
     2581 aatcgggata tcgttgacga tcagttgatc ggatttgatg cgcacccggt cccccggcaa
     2641 gcccaccagt cgcttgatgt agttgatggc cggatccggc ggccagtgaa acaccaccac
     2701 atcaccccgt ttgggctccc cgatcggcac caccttggtg tccagcaccg gcaatcgcaa
     2761 tccataggcg aacttgttga cgatgatgaa atcgccatcc agcagcgtcg gcatcatgga
     2821 gtccgacgga atgcggaacg gctcgaacac gaacgcgcgc accaccagta cgatcagcgc
     2881 gaccgggaaa aagctgcggg ccccgtccac ggtcaccggc aacggcacgc tggccgcgtc
     2941 caccccgcgc gcctgggccg cgcgcgcgcg catcggcgca agcagcaggc tgtccagcag
     3001 ccacacgaga ccggtggcgg ccaccacgat gaccagaatg aggctgaaat ccagtcgctc
     3061 ggacatcagc aggcgcagta ccgcgccgac gcatccgatc ggcagcagga agtgcacggc
     3121 gctcatgaac gagctcttgc tggcgtcggc ccccggcgca cgcgatggtc gcaggaacca
     3181 gtcctccacc accgcgatga caccggtcac caccgccact atcgtcagaa tcgtcagcaa
     3241 ctgtgtcagc atcgcgaatc cctttatttg cgcccgactc tgaggaccgc gaggaacgcc
     3301 tcctgcggaa tttccaccga acccacctgc ttcatgcgct tcttgccctc tttctgcttt
     3361 tcaagcagct tgcgcttgcg cgacacgtcg ccaccgtagc acttcgccag cacgttcttg
     3421 cgcagcgcct tgaccgtgga acgcgcgatc acgtgcgtgc cgatcgccgc ctgcaccgct
     3481 acttcgaaca tctgtcgtgg aatgagttcc tgcatggtgt cgaccaggtc gcgtccgcgt
     3541 tcataggcgt gttcacggtg cacgatgatc gacagcgcat cgaccgggtc gccgttgatg
     3601 agaatatcaa gcttcaccag cggggcgacc tgaaagtggc tgaacacgta atcaaacgat
     3661 gcatagccgc gggtcgcgga cttcagacgg tcgaagaaat cgagcacgac ctccgccagc
     3721 ggtagctcgt actgcagcga tacctgcgag ccgaggtaca gcatcttctt ctgcacaccg
     3781 cgcttctcgc tgcacagtcc cagcactccg ccaacgtagt ccggcggcac caggatactg
     3841 gcagtgatga tcggctcgcg gacctcagcc acttcattgg cgggcggcag cttggacgga
     3901 ttgtctacgt attcgagcgt gccgttggtg cgcgccacct cataaatgac ggtcggcgca
     3961 ctggtgacca ggtcgatgtt gtattcgcgc tccaggcgct cctgaacgat gtccatgtgc
     4021 agcaggccga ggaaaccgca gcgaaacccg aaccccagtg cggtcgagac ttccggctca
     4081 taatgcagcg cagagtcgtt gagcttgagc ttggcgagcg cgtcgcgaaa gctctcgtag
     4141 tcttcggcat tcaccggaaa cagaccggca aatacgcgcg gctgaatctg tttgaagccc
     4201 ggcaagggtt cgaccgcggg acgcgcctcg agcgtgatgg tgtcgcccac tggcgcaccg
     4261 tcgatttcgc ggataccggc gatcacgaac ccgacctcac cggcgctgag ggtttccgag
     4321 gccaccgatt tcggcgtgaa gcgcccgagc ttgtcgatgt tgtgcgcccg acccgtggac
     4381 atgatgcgaa tcttgtcgcc ggtgcgcagc acgccgttca cgatccgcac cagcgacacc
     4441 acgccgacgt agttgtcgta ccaggaatcc acgatcaggg cctgcaccgg cgcttcggta
     4501 tcacccttgg gcgcgggaat ccgatggatg atcgcttcca gcagctccgg gaccccttcg
     4561 ccggtcttgg ctgagacacg caccgcgtcg tgcgcctcga tcccgatgat ctcctcgatc
     4621 tccttgatga cccgatccgg atcggccgac ggcaggtcaa tcttgttgat gacaggcagg
     4681 acttccagac cctgctcatg cgcggtgtaa cagttggcca cgctctgcgc ttcgacacct
     4741 tgcgaggcgt cgaccaccag cagcgctccc tcgcaggcgg caagactgcg ggagacctca
     4801 taagagaagt ccacatgccc gggggtgtcg atcaggttga gctgataggg ctggccgtcg
     4861 tgcgcctggt agctgagcgt gacgctctgg gccttgatgg tgatgccgcg ctcgcgctcg
     4921 aggtccatgg agtcgagcac ctgctcctgc atctcgcgcg cctcgaggcc tccgcacagc
     4981 tggatcagtc ggtcggcgag cgtcgatttt ccgtggtcaa cgtgcgcgat gatggagaaa
     5041 ttgcgtatgc gttgcatcga tggggtcagg ggtcaagggc ggcggattat agcctcaacc
     5101 gcgccgagct taggtcctaa gcgcccgcaa cagctcctgc cggtccagtc gtacggcgca
     5161 gatcggcacc cgctccatca tcagcaccgg aatcttcagg ccatagcggc gttgcagctc
     5221 cggatccgaa tccacgttca ctacttgaat cggcggcagc ggatactcgg cgcgcagcgc
     5281 ctcgagctgt gcgagcatgc tctcgcacag cccacactcc gggcgcatca gcagcaccag
     5341 tgtcgtcaat ggcgcggcgg aacgggattg gaggacggcc cgaacgccgt catcggcggt
     5401 gaggcgccga aggcgctggc gggcgcgcca accgggttcg aacggggcat gttgagcata
     5461 ccgcgatgct cgaagctgcc ctccggctgg gcactcgccg gggtctgatc gaaaatcgag
     5521 ccggtatcca tggaacgcag cgccagcgcc aacggcagct caatcgcctc ataggtgtag
     5581 accggactgg gcaggttggt ggccgtgcga atcgacagcg caatcgcgcg caccgtgtcc
     5641 ggcggcactt cacccaccgc ggtcacccga tacccctgaa ccgtcgtcga ataggccgag
     5701 ctggtcccca gccgcgcggc atcatcgcgc tgcgccgtgc ccggatcgcg tccgacctcc
     5761 acgaacaccg acacggaggc catgccatcg gtaaagacca gatgctcgac cggcccgccc
     5821 ggcagcacct ggcgcgcgct ggtcgtcatc gtgaagccgg gcggcagcat cgcggcctgc
     5881 cacgacaccg acagcggact ggtgtcggtg ctgtcgttgt cctggtgcac gaacgtgaaa
     5941 ccacgcgtgt ccaccgcagg ctgcagttcg cgctcggaga tgtgccgctg cagacgaaga
     6001 tcagtgaata cgagctgctc cagcacctcg ccgctgcggc ttcgcagctg agtcttcagc
     6061 ggcatcgcgc tcgcttcgtc cacccagacc cggtagccgt agcgcagctc gtccatcggc
     6121 gtgatggcga tcacgcgcac cggacgcccc gaagcgcggg tcctggtcag ttcggtgatc
     6181 tggtattgac cggccgcccc cgcctccagt cgcggcagac tcgccagcaa cagaccgctg
     6241 tcgctgctct tttcgaccag cacgacgcgc ttgtcgggca gataacaggt gagctgggtg
     6301 ccgcgtcgga tgaactcgcg tcccgagccg tccatggata acagtcgttc ggacaccccg
     6361 tcgctgccaa ctcgatggat gacccgcagc gtctcggtgt cacccccgtg ctcgtgcacg
     6421 aagacccctt cgtagttgag tttggacagc gcctgatcgg tgcgcgccag ccagtagcgt
     6481 ggatcgtcgt ccgctgcctg agcaatcccg ctcaacagca gcgccgcgca acccccggcg
     6541 gcccagagcg tcgccgcgcc ggttgcctgg ctagcgaccc gtggtacccg tcgcttgcgc
     6601 cgcattgccc tgacccgccc tggcactacc ggcctgtgcg gcattggccg ctgcaggttc
     6661 ggcaatctct tcgctgctga ccagcgccga cagcaggctc cggcgcatca gcggcgtgga
     6721 atattcgctg tgggcgacga tgaagtcggc ca
//
LOCUS       DATMRM010000145         7368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1646068, whole genome shotgun sequence.
ACCESSION   DATMRM010000145 DATMRM010000000
VERSION     DATMRM010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7368
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646068"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1559)
                     /gene="cysG"
                     /locus_tag="VNZ06_04285"
     CDS             complement(144..1559)
                     /gene="cysG"
                     /locus_tag="VNZ06_04285"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF007922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HWW20001.1"
                     /translation="MEYLPVFLNLRDRLTLVVGGGAMAARKVELLLKAQARVRVVAPR
                     LHPELALFRDAGRIEHLPLAFEPQHFSDVAFAIAATDSRAVNQAVASTGAARGVFVNV
                     VDDAIGSSAVMPALVDRSPIIVAIGTAGQSPTLARRLRAQLEAMLPERLGELARWAGA
                     VRERVRKGLPDADRRRHFWDQLFAGNLASLVLAGRVRDADQLLEQQLQHACADTLAGR
                     GEVYLIGAGPGDPDLLTLRALQLMQQADVVLYDRLVSAAVLDRVRRDARRIDVGKQSG
                     RHRVTQDRIHSLLLEHARQGLRVARLKGGDPFIFGRGGEEIDLLQRAGIPVIVVPGVT
                     AALGAAASAQVPLTQRGLAPSVTFVTATGEGASELNWAALSAPSQTIVFYMGVAQLPR
                     IVEHLHAHGAPADRPVAIVERATLPDQRVVAGSLGNIVEMAQRAQVAAPALLIVGEVA
                     SRAVQSAQQLPFQWMAGVART"
     gene            complement(1582..2313)
                     /locus_tag="VNZ06_04290"
     CDS             complement(1582..2313)
                     /locus_tag="VNZ06_04290"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HWW20002.1"
                     /translation="MSPRAHSPSIDDSLAATIESSLMLLRQAVNDYGRVVYANSLGSE
                     AMVLTDLIWNHVPGIDIVSIDTGRLPEETLGLLERLERRYNRRIKVYYPDARSVERYV
                     RENGINGFYNSLGQRLSCCEIRKVEPFKRAVAGYSAWVTGVRREQSESRRQTPLIERD
                     AQVGIAKISPLLDWTDEQIWTYIHAHQLFYSPLHDRGFPSIGCAPCTRAIEPGQDQRS
                     GRWWWEQPDSRECGLHPRRRSVAVS"
     gene            2462..3439
                     /gene="hemB"
                     /locus_tag="VNZ06_04295"
     CDS             2462..3439
                     /gene="hemB"
                     /locus_tag="VNZ06_04295"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HWW20003.1"
                     /translation="MSSLNLRRLRQDSHVRALTREVHLQLDQLIQPLFVGETNLRPEP
                     IPGLTGVHQDTPDSLLRQVDQDLRAGVDKFLLFGVPQARSERHIDWSFTAGQIQALKS
                     RFGRDIWLAIDVCLCSSTPHGHCGVLNAEGDHLDNDASLVELVQAALAYAKAGADCVA
                     PSDMMDGRIGAIRQALDRDGLERTVLMSYAVKFQSTLYGPFRVAADAAPKGGTVLKDR
                     ATYQLDPARPGDAWLCAQRDDAEGADILMVKPGLPYLDLLRELSREFRKPWAVYHVSG
                     EFAALEALAASGLATRAALHREIMTAFRRAGASMIITYGARFAHEWLSA"
     gene            3436..4722
                     /locus_tag="VNZ06_04300"
     CDS             3436..4722
                     /locus_tag="VNZ06_04300"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HWW20004.1"
                     /translation="MSGNDARSAELFARARRVSPGGVHSPVRAFRGVGGTPRFMTGGA
                     GALLRDVDGREYVDYCMAFGPLIFGHADPGIRAAAEAALGRGWSLGTAEPYSLELAEL
                     ISTRLPWVESIRFVNSGTEAVMSALRVARAATRRSKILKFEGCYHGHTDSMLLRAGSG
                     LADAPLPDSAGLAPDVVQETLVSVLDSESALEALFARHGSEIAAVIIEPLPANYGLLP
                     QRTEFLQTLARLARSHGSLLIFDEVISGFRVCFGGYAALSGITPDLVTYGKVIGGGFP
                     VGAYGGRRDLMELVAPAGPVYQAGTLSANPLAMCAGLATLQRLTDTAVYARLEQLGAR
                     LERSLSNLPHLAVQRVGSIFWMCHAKQQPPTVPMRAPSALPADAGKSFAPLFHSLLDG
                     GVYLAPSAFEVGFLSAAHTEAQIDALAQRVRQHCAP"
     gene            complement(4726..6846)
                     /gene="glgX"
                     /locus_tag="VNZ06_04305"
     CDS             complement(4726..6846)
                     /gene="glgX"
                     /locus_tag="VNZ06_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HWW20005.1"
                     /translation="MAGELPTVPLPGTPYPPGATSDAGGTNFAVFAGGAEQVDICLFD
                     PAGRQELRRMALRECTDGVWHGYLPSVAPGQLYGFRAQGPYQPEQGLRYNPNKLLLDP
                     YARRLAGALRWSDAVFGYRCTSPRADLTIDRRDSASCMPKAVVTAEQFDWGEDKPPRV
                     PWSDTVIYEAHLRGLTLRAEAIPERLRGTAAALGHEHTVGYLQRLGITAVELLPIHAI
                     VNDRVLVDKGLCNYWGYNTLGFFAPEPRYLADGSLGELKAAIKALHAAGIEVLLDVVY
                     NHTGEGNERGPTLSLRGLDNVAYYRLEAGNPRLYVNDTGTGNTLNFSHARVIALTLDS
                     LRYWVQEFHVDGFRFDLSATLGREPTGYDAGSGFFDALMQDPVLARVKLIGEPWDLGP
                     GGYQVGNHPPGMSEWNDKFRDDVRRFWRGDPGLRGAIAARLQGSAELFDHHGRRPWAS
                     LNFITAHDGYTLQDWVSYEKKYNEANGENNRDGSDNNLSRNWGVEGPTEDPAIRITRE
                     RVKRAMQAMLLFSHGTPMLLGGDEFGRTQHGNNNAYAQDSDISWFDWVQALSSSGREQ
                     CGYVMRLLRLRREYSTLRSAYYQHGRIEPLPQVKDVEWFDENGDIMRGEDWQNPQGRL
                     LCLRRAARLDERRAEVTLILTNNGMQAHAFQLPDPTFRWWLRINSDDPHGAEVELELG
                     TVEVAPHSVQLLTAIVEWSAPPNP"
     gene            complement(6871..>7368)
                     /locus_tag="VNZ06_04310"
     CDS             complement(6871..>7368)
                     /locus_tag="VNZ06_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HWW20006.1"
                     /translation="GKKLLFMGGEFGQDWEWNHDGQVDWVALEHPLHAGILRWVSDLN
                     HLYRQEPALHVFDCDGAGFRWINADDCANSVYAWYRSGHAEAAPVIAVCNFTPVPRRC
                     YRIGAPRAGLWREVLNSDSELYGGSNMGNGGWLETSTHGFHGQPFSLELTLPPLATVL
                     LRYGS"
BASE COUNT         1261 a         2466 c         2345 g         1296 t
ORIGIN      
        1 gcgcgcagcg cggtccttga ccgagcctcc cggattcatg aactccgctt tgccgtaaat
       61 gtcgcagccg gtctcctcgc tgacgcggcg caggcggatc atcggtgtac gaccgacggt
      121 atcgacgaag tccttcagga cattcatgtc ctcgcgactc ctgccatcca ctgaaacggc
      181 agctgttgcg cgctctgcac ggcgcgtgag gccacctcac ccacgatcag cagcgcggga
      241 gctgccacct gcgcgcgctg cgccatctca acgatattgc ccagggaacc ggcaaccacg
      301 cgctgatccg gtagcgtcgc gcgctcgacg atcgcgaccg gacgatcggc gggagctccg
      361 tgtgcgtgca aatgttccac gatccgcgga agctgcgcca cccccatgta gaacacgatg
      421 gtctgcgagg gtgcggacag cgccgcccag ttgagctccg acgcaccctc gcctgtcgct
      481 gtgacgaagg tgaccgaggg cgccagcccg cgctgtgtca gaggaacctg cgcgctggcg
      541 gccgcaccca gcgccgcggt gacacccgga accacgatga ccggtatgcc cgcccgctgc
      601 agcagatcga tctcctctcc accgcgtcca aaaatgaatg gatcgcctcc tttgagtcgc
      661 gcgacccgca ggccctgccg ggcatgttcg agcagcagcg aatggatgcg atcctgagtc
      721 acccgatgcc ggccagactg cttgccgaca tcgatgcgtc gcgcatcgcg acgcacgcga
      781 tcgagcactg cggcgctcac cagtcggtcg tacagcacca catcggcctg ttgcatcagc
      841 tgcaatgcac gcagcgtcaa cagatcggga tcgccgggac cggcgccgat caggtacacc
      901 tcgccgcgac ccgccagagt gtcggcgcac gcgtgctgca actgctgttc gagcagctga
      961 tccgcgtcgc gcactcgccc cgccagcacc aggctggcaa ggttgccggc aaacagctgg
     1021 tcccagaaat ggcgtctgcg atccgcgtcc ggcagcccct ttcgcacccg ctcgcgtacc
     1081 gcgccggccc aacgtgcgag ctctcccagc cgctcgggca gcatggcctc gagttgtgcc
     1141 cgcagccgac gtgccagcgt cggtgactgg ccggcggtgc cgatcgcgac gatgattggc
     1201 gagcgatcga cgagcgccgg catgaccgcg ctggagccga tcgcatcgtc caccacattc
     1261 acgaacacgc cccgcgccgc gccggtgctc gcgacggcct ggttgacggc gcgcgaatcg
     1321 gtcgcggcaa tggcgaacgc cacatcactg aagtgctgcg gctcgaatgc cagcggcaga
     1381 tgttcgatgc ggccggcatc acgaaacaac gccagctcgg ggtgcaaacg gggggccacc
     1441 acgcgcacgc gtgcctgcgc cttcagcagc agttcgactt tgcgtgccgc catggcaccg
     1501 ccgccgacca ccagcgtcag gcgatcacgc agattgagga ataccggcaa atactccatg
     1561 ggccggtgat cttagcgaat gtcagctcac tgccaccgaa cgccgtcgtg gatgcaggcc
     1621 gcattcacgc gaatcaggct gttcccacca ccagcggccg gaacgctggt cctgcccagg
     1681 ctcgatcgcc cgcgtgcacg gggcacagcc gatgctagga aagccgcgat catgcagcgg
     1741 gctgtagaac agctgatggg cgtgaatgta ggtccagatc tgctcgtcgg tccaatcgag
     1801 cagtggactg attttcgcaa taccgacctg tgcgtcacgc tcgatcagcg gtgtctgtcg
     1861 gcggctctcg gattgctccc ggcgtacgcc ggtcacccag gccgaatagc cggccaccgc
     1921 acgcttgaac ggctcgacct ttcggatttc gcagcagctc aggcgctgac ccaggctgtt
     1981 gtagaagccg ttgatcccgt tctcacgtac ataacgctcg accgatcggg catcggggta
     2041 gtagaccttg atccggcggt tgtaacgacg ctccaggcgc tcgagcagtc ccaaggtctc
     2101 ttccggcagg cgcccggtat cgatcgacac gatgtcgatc ccggggacgt ggttccagat
     2161 caggtcggtc aggaccatcg cctccgaccc caggctgttg gcatagacga ccctgccgta
     2221 gtcattcacc gcctgccgca gcaacatgag gctgctctcg atggtggccg ccaggctgtc
     2281 gtcgatgctg ggggaatggg cgcgcggtga catagcggtg atattgcccg ccagtcttat
     2341 aacattggct tatgagttaa gttatgaggc aacctcaagc gttcaacagt gacgccgttc
     2401 aagctcattg tgacaatgcg tgaccgcggc acgggtggat aacgtcacac ttcgggtacc
     2461 catgagctca ctgaatttgc ggcgcctgcg ccaggattcg cacgtgcggg cactgacccg
     2521 cgaagtgcac ctgcaactgg accagctgat ccagccgtta tttgtcggcg aaaccaacct
     2581 gcggccggag ccgataccgg gactgaccgg agtacaccag gacaccccgg attcactgct
     2641 gcgtcaggtg gatcaggacc tgcgggccgg cgtcgataaa ttcctgctct ttggcgtgcc
     2701 acaggcccgc agcgaacgtc acatcgactg gtcgttcaca gcgggccaga tccaggcgct
     2761 gaaatcgcgg ttcggccgcg acatctggct cgccatcgat gtctgcctgt gctcctcgac
     2821 accgcacggt cattgcggtg tgctcaatgc cgagggtgat cacctcgaca atgacgcgag
     2881 cctggtggag ctggtgcaag ccgcgctcgc ctatgcgaag gcgggtgcgg actgcgtggc
     2941 gccgagcgac atgatggacg ggcgcatcgg cgcgatccgc caggcgctgg accgggacgg
     3001 cctggagcgc acggtgctga tgagttatgc ggtcaagttc cagtccacgt tgtatggacc
     3061 tttccgggtc gctgccgacg cggcacccaa gggcggcacg gtcctcaagg atcgcgccac
     3121 gtatcagctc gaccccgcgc gccccggcga tgcctggctg tgtgcccagc gcgatgatgc
     3181 ggaaggggcc gacatcctga tggtcaagcc gggactgccc tacctcgatc tgctgcgcga
     3241 gctgtcgcgc gaattccgca agccctgggc cgtgtaccac gtcagcggcg aattcgccgc
     3301 cctggaagca ctggcagctt cgggcctggc aacgcgcgcg gccctgcacc gtgaaatcat
     3361 gacggcattt cgccgtgccg gcgccagcat gatcatcact tatggcgcgc gcttcgcgca
     3421 cgagtggctg tcggcgtgag cggcaacgac gcccgctccg cggagctgtt cgcgcgtgcc
     3481 cgacgcgtat cacccggcgg tgtgcattcg ccggtgcgtg catttcgcgg cgtgggcggc
     3541 acgccgcgtt tcatgaccgg cggtgccggt gcgctgctgc gcgatgtgga cggccgggag
     3601 tatgtggatt actgcatggc cttcggcccg ctgatcttcg gtcacgccga cccggggatc
     3661 cgcgctgcgg ccgaagcggc gctggggcgc ggctggagtc tggggacggc cgaaccctac
     3721 agcctcgagc tggcggagct gatcagcacg cgtctgccct gggtcgagtc gatccgcttc
     3781 gtgaattcgg gtaccgaggc ggtgatgtca gcgctgcgcg tggcgcgggc ggccacccgt
     3841 cgctccaaaa tcctgaaatt cgaaggctgc tatcacggcc ataccgattc gatgctgctg
     3901 cgtgcgggct ctggcctggc cgatgcaccg ctgccggaca gcgcggggct tgcacccgac
     3961 gtcgtgcagg aaacgctggt cagcgtgctg gacagcgaga gtgcgctcga ggccctgttc
     4021 gcgcgtcatg ggagtgagat cgccgcggtc atcatcgagc ccctgccggc gaactacggc
     4081 ctgttaccgc agcggaccga gtttctgcaa accctggcac ggctggcgcg ctcgcatggc
     4141 tcattgttga tcttcgacga ggtgatctcc ggctttcgtg tgtgctttgg cggatacgcc
     4201 gccttgagcg gcatcacacc ggatcttgtg acctatggca aagtcatcgg cggcggcttt
     4261 ccggtgggcg cgtatggcgg gcggcgcgac ctcatggagc tggtcgcccc tgccgggccg
     4321 gtctatcagg ccggcacgct gagcgccaat ccgctggcga tgtgcgccgg cctcgccacg
     4381 cttcagcgtc tgaccgatac ggcggtctat gcccggctcg agcagttggg tgcgcgactg
     4441 gagcgctcgc tcagcaacct gccgcacctg gccgtgcagc gcgtgggctc catcttctgg
     4501 atgtgccacg ccaagcagca gccacccacg gtcccgatgc gcgccccgtc ggccttgccc
     4561 gcggacgccg gcaagagctt cgcgccgctg tttcattcgc tgctcgacgg cggcgtctat
     4621 ctggcgccca gcgcgttcga agtcggattc ctgtcggctg cgcacaccga ggcgcagatc
     4681 gatgcgctgg cgcagcgcgt acgccagcac tgcgcgccgt agctcctacg gattcggcgg
     4741 cgcggaccac tccacgatcg cggtcagcag ctgcacgctg tgcggcgcca cctccaccgt
     4801 gccgagctcc agttcgacct cggcaccgtg cggatcgtcg ctgttgatac gcagccacca
     4861 gcgaaaggtt gggtcgggca gctgaaaggc gtgcgcctgc atcccattgt tggtcaggat
     4921 cagcgtcacc tcggcgcgtc gctcatccag acgcgccgcg cgtcgcagac acagcaagcg
     4981 cccttgcggg ttctgccagt cctcaccgcg catgatgtcg ccgttctcgt cgaaccattc
     5041 gacgtccttc acctgcggta gcggctcgat gcgcccgtgc tgatagtagg cactgcgcag
     5101 cgtgctgtac tctcgccgca atcgcagcag gcgcatcaca tacccacact gctcgcggcc
     5161 gctgctggac agcgcctgca cccagtcgaa ccacgagata tccgagtcct gcgcataggc
     5221 gttgttgttg ccatgctgag tacgcccgaa ttcatcacca cccagcagca tgggcgtgcc
     5281 gtgcgagaac agcagcattg cttgcatcgc acgcttgacg cgctcgcgcg tgatccggat
     5341 ggcggggtct tcggtcggtc cctccacgcc ccagttgcgg ctgagattgt tgtcgctgcc
     5401 gtcgcgattg ttctcgccgt tggcctcgtt gtactttttc tcgtagctga cccagtcctg
     5461 cagcgtatag ccatcatgtg cggtgatgaa gttgagcgac gcccagggcc ggcgtccgtg
     5521 gtgatcaaac agctccgcgg aaccctgcag gcgcgccgcg atcgcaccgc gtagcccggg
     5581 atctccgcgc cagaatcggc gtacatcatc gcggaatttg tcgttccatt cagacatccc
     5641 gggcggatga ttgccgacct gataaccacc tgggcccaga tcccacggct caccgatcag
     5701 cttgacgcgc gccagtaccg ggtcctgcat caacgcgtcg aagaaaccgc tgcccgcatc
     5761 gtaaccggtg ggctcccgtc ccaaggtcgc gctcaggtcg aaccgaaacc cgtcgacgtg
     5821 aaactcctgc acccagtagc gcagcgaatc cagggtcaac gcgatcacgc gcgcgtgtga
     5881 gaaattcagg gtattgccag tgccggtgtc gttgacgtaa agacgcggat tgccggcctc
     5941 cagccggtag taggcaacgt tgtccagacc gcgcaagctc aaggtgggcc cgcgctcgtt
     6001 gccttcgccc gtgtggttgt atacgacatc gagcaacacc tcgatgccgg ccgcatgcag
     6061 cgctttaatg gctgccttca gctcacccag cgagccgtcg gccagataac gcggttcagg
     6121 cgcgaagaac cccagcgtgt tgtaacccca gtagttgcac agacctttat cgaccagcac
     6181 ccgatcgttg acgatcgcgt gaatgggcag cagttcgacc gcggtgatac ccagacgctg
     6241 cagatagccc accgtgtgtt cgtgacccag cgccgcggcc gtgccgcgca accgttccgg
     6301 tatcgcctcc gcgcgcagcg tcaagccgcg cagatgtgct tcatagatga cggtatcgga
     6361 ccagggcacg cgcgggggtt tgtcctcacc ccaatcgaac tgctcggcag tgaccaccgc
     6421 cttcggcatg caggaggcgc tgtcgcgccg gtcgatggtc aggtcggcac gcggcgaggt
     6481 gcagcgatag ccgaagacgg catcgctcca gcgcagcgca ccggcgagcc ggcgtgcata
     6541 cggatcgagc agcagcttgt tagggttgta gcgcagtccc tgttcgggtt gatacgggcc
     6601 ctgtgcgcga aagccgtaga gctgtcccgg cgcgacactg ggcaggtagc cgtgccagac
     6661 gccgtcggtg cactcgcgca gtgccatgcg ccgcaactcc tggcgtcccg ccggatcgaa
     6721 caggcagata tccacctgtt cggcgccgcc ggcgaagacc gcgaagttag tgccgcccgc
     6781 atcgctggtg gcgcccggcg gataaggggt cccgggcagt gggaccgtcg gcagctcgcc
     6841 cgccatgcac ggttttcctt gtcgatgaag ctatgaaccg tagcgcaaca ataccgtggc
     6901 cagaggcgga agcgttagct cgagtgaaaa cggctggcca tgaaatccgt gcgtgctggt
     6961 ctcgagccag ccgccgttgc ccatgttgga gccaccgtac agttcggaat cggagttgag
     7021 gacttcgcgc cagagaccgg cgcgcggtgc gccaatgcga tagcaccgac gcggcaccgg
     7081 ggtgaagttg cacaccgcga tcaccggcgc cgcctcggcg tggccgctgc gataccaggc
     7141 gtacacgctg ttggcgcagt catcggcatt gatccagcga aaacccgcgc catcacaatc
     7201 gaacacgtgc agcgccggct cctgacgata caggtggttc aggtcggaca cccatcgcag
     7261 tatgcctgcg tgcagtggat gctccagtgc cacccaatca acctgcccgt cgtgattcca
     7321 ctcccagtcc tggccgaact ctccgcccat gaacaacagc ttcttgcc
//
LOCUS       DATMRM010000146         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3478294, whole genome shotgun sequence.
ACCESSION   DATMRM010000146 DATMRM010000000
VERSION     DATMRM010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3478294"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VNZ06_04315"
     CDS             <1..197
                     /locus_tag="VNZ06_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20007.1"
                     /translation="GRVGMVSGLFFGVAFGMAGIGAAALGELADLTSIEFVYRVCAFL
                     PLIGLLTAFLPNIEQTRRAR"
     gene            complement(351..1790)
                     /locus_tag="VNZ06_04320"
     CDS             complement(351..1790)
                     /locus_tag="VNZ06_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HWW20008.1"
                     /translation="MAAPDDTPSRDDGTAATSGDAVIEALRVENALLRAENAALLQRL
                     ADLERRLGLNSSNSGKPPSSDGLKKPPRVSSLREPSTGGQKGHPGTTLRRTETPDATV
                     DHYPEACAACGEPLTAAMATGHVARQVFDLPEPTPLIVTEHRAHDCRCLSCGTQTRAA
                     FPEGVTAPVQYGKRIGAFVLYLLHYQLLPEKRLAALMADLFGVKLVTATIARISQDCA
                     HRFMGFADAVRDHVAAAPVKHLDETGFRIGGKTQWLHIASTVWLTFYRVSAKRGSLLP
                     KVTGIVVHDHWKPYYTMTGVLHALCNAHHLRELKALVEIEKEDWARKMQRLLRRACHA
                     ANFAHQQGAPLKAGLIALLDRCYDTILAEGLAFHQAQPALAKARRRGRQPRRVGHNLL
                     LRLSTRKLDVLRFLTDPSVPFTNNLAEQDGRMMKLRQKISGGFRSEDGAKDFAVIRSV
                     LSTARKQGWNMLQTLTTAPTRLITDIRLA"
     gene            complement(1855..>2150)
                     /locus_tag="VNZ06_04325"
     CDS             complement(1855..>2150)
                     /locus_tag="VNZ06_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20009.1"
                     /translation="DRGTGRLAVDERSNHGLLTLRDKPPRYSGMTDRIGTAKATRAGL
                     GRDEHPGPAGIRRHRVATSGQEKFDLVRSVTPTKLIFVLSLNVSVVPAAVTLV"
BASE COUNT          371 a          674 c          684 g          421 t
ORIGIN      
        1 ccggacgagt cggcatggtc tcgggcctgt tcttcggcgt tgcgttcggc atggcgggta
       61 tcggcgccgc ggccttgggc gagctcgccg atctgaccag tatcgagttc gtctaccggg
      121 tctgtgcgtt cctgccgctg atcgggctgc tcacggcgtt cctgccgaac atcgagcaga
      181 cacggcgagc acgttagcgc gcccgacttc gcaaccgtaa atccgtgcgc cacgactcac
      241 gcgtgcattc gctcgtgaat ttcgcgcacc cacactgcac gaaacgtcgc tgacaagtgg
      301 atcgtaatta tgtaaaaccc tcgtaacagc ccaggtagcg aagaggccga tcaagccagc
      361 cggatgtctg tgatcaggcg cgttggagca gtggtcaagg tctgcagcat attccaaccc
      421 tgctttctgg cagtggagag aaccgagcgg attacggcaa aatcctttgc accatcctcg
      481 gagcgaaatc cgccggagat cttctggcgc agtttcatca tgcgcccatc ctgctccgca
      541 aggttgttgg tgaagggcac gctgggatct gtcaggaagc gcagaacgtc aagcttgcgg
      601 gtgctcaagc gtagcaagag gttgtggcca acccgtcgcg gctgacggcc acggcgtcgg
      661 gcttttgcca gtgcgggctg tgcttggtgg aacgccaggc cttccgcgag gatcgtgtcg
      721 taacatcggt ccaacaaggc gatcaggccc gccttcagtg gcgcgccctg ttggtgcgca
      781 aagttggccg cgtggcaggc gcggcggagc agccgctgca tcttgcgtgc ccagtcttcc
      841 ttctcgatct cgaccagcgc cttcagttcc cgcagatggt gcgcattgca aagcgcgtgc
      901 agcacgccgg tcatcgtgta gtacggcttc cagtgatcgt gcacgacaat gcccgtgacc
      961 tttggcaaca ggctgccccg cttcgccgag acgcgataga aagtgagcca gacagtcgag
     1021 gcgatgtgca gccactgcgt cttgccgcca atgcggaagc cggtctcatc caggtgcttg
     1081 accggcgccg ctgccacgtg gtcgcgcacg gcatcggcaa aacccatgaa gcggtgcgca
     1141 caatcctggc tgatccgggc gatggtcgcg gtgaccagct tgacgccgaa caggtcggcc
     1201 atcagtgcgg cgagacgctt ttccggcagc aactgatagt gcagcagata cagcacaaac
     1261 gcgccgatcc gtttgccgta ctgcaccggt gcggtcaccc cctcggggaa cgccgcccgc
     1321 gtctgcgtgc cgcagctgag gcagcggcag tcatgtgcgc ggtgttcggt gacgatcagt
     1381 ggcgtcggct cggggagatc gaacacttgt cgggcgacat ggcccgttgc catggccgca
     1441 gtcagcggtt caccgcacgc cgcgcacgcc tctggataat ggtcgacggt ggcatcaggg
     1501 gtttcagtcc ggcgaagcgt cgtgcccgga tggcccttct ggccgccggt cgacggttcc
     1561 cgcagactgc tgacccgcgg cggtttcttc agcccgtcgc tggacggtgg cttgccactg
     1621 ttgctgctgt tcagtccgag acggcgctcc aggtccgcca gccgctgtaa cagtgcagca
     1681 ttctccgcac gaagcagcgc attctcgacc cgcaatgcct ctatcaccgc atcgccactt
     1741 gtcgcggccg taccgtcgtc tcgtgagggt gtgtcgtcgg gcgctgccat cgacacgttg
     1801 actcgtactc gcccgtgacc tgtcaatggt ccccatcaga ggcgccctag gcagttacac
     1861 caatgttaca gcggctggaa caacgctgac gttgagcgac agcacgaaga tcagtttcgt
     1921 gggtgtcact gatctgacca agtcgaactt ctcctgacct gaagtggcga cacgatgacg
     1981 cctgatgcca gcgggaccgg gatgctcgtc ccggcccagt ccggcgcgcg ttgctttggc
     2041 cgtgccgatc cggtccgtca ttccagagta acggggtggt ttatcgcgga gcgtgagcaa
     2101 cccgtgattg ctgcgctcgt cgacggcgag ccggccggta ccgcggtcat
//
LOCUS       DATMRM010000147         5200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1320026, whole genome shotgun sequence.
ACCESSION   DATMRM010000147 DATMRM010000000
VERSION     DATMRM010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320026"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /gene="glyA"
                     /locus_tag="VNZ06_04330"
     CDS             <1..222
                     /gene="glyA"
                     /locus_tag="VNZ06_04330"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645593.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWW20010.1"
                     /translation="NKNAVPNDPRPPTVTSGLRLGTPAATTRGFGEAEVRRVAGWIAD
                     ILDGAGSEDAIERVRPQVIELCRRFPVYS"
     gene            247..687
                     /gene="nrdR"
                     /locus_tag="VNZ06_04335"
                     /pseudo
     CDS             247..687
                     /gene="nrdR"
                     /locus_tag="VNZ06_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006878018.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
     gene            774..1880
                     /gene="ribD"
                     /locus_tag="VNZ06_04340"
     CDS             774..1880
                     /gene="ribD"
                     /locus_tag="VNZ06_04340"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HWW20011.1"
                     /translation="MNPLDQDLSHMRRALELARRGLNSADPNPRVGCVIVRDGRVVGE
                     GWHERAGEPHAEVHALRDAGSEAEGSTVYVTLEPCAHTGRTPPCTSALIAARVQRVVY
                     AIGDPNPLVDGRGAALLRAAGIETSSDVLAGEARALNPGFFKRMQCGMPWVRVKLGAS
                     LDGRTALANGQSRWITGKQARQDAQRFRARSSVVLSGSGTVLADDPALNVRIDEATRQ
                     PMRVVLDSSLRVPPQARMFDREGAALVFTASEDQARRAELTRRGVAVESVPRAVEGGL
                     ELEAVLRRLAQLQANEVWVEAGARLAGALLHADLVDELIVYLSPSLLGPQARPLVDLP
                     QISSLEMRLALTYTEVTTIGADLRLTAVPARRAL"
     gene            1902..2435
                     /locus_tag="VNZ06_04345"
     CDS             1902..2435
                     /locus_tag="VNZ06_04345"
                     /inference="COORDINATES: protein motif:HMM:NF022605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2937 family protein"
                     /protein_id="HWW20012.1"
                     /translation="MWILRAFLDRVILLAGVVIAGCVPGFIMQYRQRLNGRLEQVVRD
                     LAPFQAIADHEHHGSLKELIEYHLQSTDPTFHQEGQAIQAMLDSAEHLRSMLDGLNSD
                     IGHQFTYLLTHYDNTLLHATWGDYRPAFALDLQGVLFALVIGAALWAAFMLIWYAVAW
                     LLGSILGNPTRANSRLR"
     gene            2488..4794
                     /locus_tag="VNZ06_04350"
     CDS             2488..4794
                     /locus_tag="VNZ06_04350"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HWW20013.1"
                     /translation="MQKKHTASLAVGGYAALIGLCLAAAAPGASAEVGAMLGFSSARA
                     AEEHALERRFDDQLDAAGVREFIRTMASQPNQVGSPHDKANAETMLQQFRSWGWSAQI
                     ESFSVLYPTPRKIALEMVAPHSYRARLHEPPVAGDKSSAITAGTLPPYNVYGADGDVT
                     AELVYVNYGGREDYQELDRRGISVKGRIVIARYGNGWRGLKPKLAYEHGAVGCLIYSD
                     PHEDGYGAGDVYPQGGWRPPDGVQRGSVADLTQYSGDPLTPGVGATADAKRLALSDVK
                     TLMKIPVMPISYADAQPLLAALTGAVAPPAWRGSLGQTYHVGPGAARVHMHVESDWGM
                     KTVYDVIARIAGSELPDEWIVRGNHHDGWVFGAWDPLAGNAALLSEAKAIGALLKSGW
                     RPRRTLIYCSWDAEEPGLIGSTEWAETHALELRAHAVLYVNSDTNARGFLEMGGSHSL
                     QRLLNEVAEGVHDAESDSSVLARLRAKLRVEAYNGGNEQIRKLAAAAASGADIPLTAL
                     GSGSDYTPFLQHLAIASVDLGFGGEADNAGVYHSQYDSFEHYERFGDPKFVFGIALAQ
                     SAGHLMLRMADADVLPLQFTGMAEVMADYVHELHQLADERREHAQRLSELRQSRAFEL
                     ASDPTRPLAAPAAESEVPFLDFAPLDNASAHLRRAARAYDEAYAEASARGFAVGAARE
                     KQLDETLRGLEQTLASDQGLPDRPWFRHLIYAPGVYTGYGAKTMPGVREAIEDHRWEV
                     ASQYVHLTAEALERYAEHVEAATALLRG"
     gene            4798..>5200
                     /locus_tag="VNZ06_04355"
     CDS             4798..>5200
                     /locus_tag="VNZ06_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HWW20014.1"
                     /translation="MRRHLFERHYVSRINWLRAAVLGANDGIVSVASLLVGVAATHVS
                     RQQALLVGVAGLVAGAMSMSAGEFVSVSSQADAERSDLERERRELEADESAERAELAA
                     IYVKRGLKPALAQEVAVQLMAQDALGAHARDE"
BASE COUNT          885 a         1605 c         1814 g          896 t
ORIGIN      
        1 aacaagaatg ccgtgccgaa cgatccgcgg ccgccgaccg tcaccagcgg cctgcgactg
       61 ggcacaccag cggccaccac gcgcggtttc ggggaagccg aggtacgccg ggttgcggga
      121 tggatcgcgg atattctgga tggcgcaggg agcgaggacg cgatcgagcg cgtacggcct
      181 caggtgatcg aactgtgtcg gcgctttccg gtttactcct agcggtgcga tgagcttcgg
      241 cagtcgatgc attgtccctt ttgcagtcac gtcgagacca aggttaccga ctcgcggctg
      301 gcggcggacg gcacgcagat ccgccgtcgc cgtgagtgcc tgagctgcgg cgagcgcttc
      361 accaccttcg agagtgccga actgctgatg ccgcgtgtca tcaagagcga cggcagtcgc
      421 gagccgttcg acgagcacaa actcaagcaa ggcatgatca aggcactcta caaacgtccg
      481 gtcagtgccg aggcgatcga tgaggcggtc agccgcattg gacaccgcct gcgggtcatg
      541 ggcgagcgtg aggtgctggc gcgctcggtc ggtgagctgg tgatggacga gctgcggcac
      601 atggatgaag tcgcatacgt gcgctttgca tcggtgtacc gcagcttcca ggatgcggaa
      661 gccttccgcg atgaaatcga gcggctgcgc catcatcggc ggcgtcgcga ggcggccaag
      721 cgtgacgagg accagttgcc gctgttgccg ggtgaaccag tcaaatccaa atcatgaatc
      781 cattggatca ggacctatcc catatgcgcc gtgcgctcga gctggcgcgg cgcggactga
      841 acagcgccga tccgaatccc cgtgtcggct gcgtgatcgt gcgcgacggc cgtgtggtcg
      901 gtgaaggctg gcacgaacgc gccggcgagc cgcatgccga agtgcacgca ctgcgcgacg
      961 ctggcagcga ggccgaaggc tcgaccgtct atgtgacgct cgagccctgt gcgcacaccg
     1021 gacgcacgcc gccgtgtacc tcggctctca tcgcggcgcg cgtgcagcgg gtggtctacg
     1081 cgatcggcga tccgaatccg ctggtcgatg gccgtggcgc cgcgctgctg cgcgccgccg
     1141 gaattgagac ctcgagtgac gtgctggccg gtgaagcgcg tgctctgaat ccgggctttt
     1201 tcaagcgcat gcaatgcggc atgccgtggg tgcgcgtcaa actgggcgcc agtctcgacg
     1261 gccgcacggc gctggccaac ggccagagcc gctggatcac gggcaagcag gcgcggcagg
     1321 atgctcagcg ctttcgcgcg cgcagctccg tcgtgctcag cggcagcggc accgtactgg
     1381 ccgacgatcc ggcgctcaac gtgcgcatcg acgaggcaac gcgtcagccg atgcgcgtgg
     1441 ttctggacag ctcgctgcgt gtcccgccgc aagcgcgcat gttcgatcgc gagggtgcgg
     1501 cgctggtgtt cactgccagc gaggatcagg cgcgacgcgc ggagctgacc cggcgcggcg
     1561 tggccgtgga gagcgtgccg cgcgcggtcg aaggcggcct agagctggaa gcggtgctgc
     1621 ggcgactggc gcagctgcag gcaaacgaag tgtgggtcga ggccggcgcg aggctcgccg
     1681 gtgcgctgct gcatgccgac ctggtcgatg agctgatcgt ctatctgtcg ccgtcgctgc
     1741 tcggtccgca ggcgcgcccg ctggtcgatc tgccgcagat ctcatcgctc gagatgcgcc
     1801 tggcgctcac ctacactgag gtgacgacga ttggtgcgga tctgcgcctg accgcggtgc
     1861 cggcgcgtcg cgcactttag actctaggag aagctgcgat catgtggatc ctgcgcgcgt
     1921 ttctcgaccg ggtaatcctg ctggccggcg tcgtcatcgc gggctgcgtg ccaggcttca
     1981 tcatgcaata ccggcaacgt ctcaatggcc gcctcgaaca ggtcgtgcgc gatctggcac
     2041 cctttcaggc gattgccgat cacgaacatc atggcagttt gaaagaactg atcgagtatc
     2101 atctgcagag caccgacccg accttccacc aggagggtca ggcgatccag gcgatgctcg
     2161 attcggctga gcacctgcgc agcatgctcg acggactgaa cagcgatatc gggcatcagt
     2221 tcacctatct tctgacccac tatgacaaca cactgctgca cgcgacctgg ggcgactacc
     2281 gcccggcgtt cgcgctggac ctgcaggggg tgctgttcgc gctggtcatc ggcgctgcgc
     2341 tgtgggcggc cttcatgttg atctggtatg cggtggcatg gctgttgggc tcgattctgg
     2401 gcaatccgac acgcgccaat tcgcgattgc gttaagattt cgctttacgg cccaccgtcg
     2461 ggccaagcac aacaacacct aattcccatg cagaaaaaac atactgcctc tctggctgtc
     2521 ggcggctacg ccgcattgat tggactgtgt ctggcggccg ccgctcccgg cgcgagcgct
     2581 gaggtaggcg cgatgctcgg gttcagctcc gcacgcgcgg ctgaggagca cgcgcttgag
     2641 cggcgcttcg acgaccagct tgatgccgcg ggcgtgcgcg agttcattcg cacgatggcc
     2701 agtcagccca atcaggtagg ttcgccgcac gataaggcca acgccgaaac catgctgcag
     2761 cagtttcgct cctggggctg gtcggcgcag atcgagagct tcagcgtgct gtatccgacg
     2821 ccgaggaaaa tcgctcttga aatggtggcg ccgcacagct accgcgcgcg cctgcacgaa
     2881 ccgccggtgg ccggggacaa gagctcggcc atcaccgccg gcacgctgcc gccgtacaac
     2941 gtctatggcg ctgacggcga tgtgaccgcc gaactggtgt acgtgaacta cggcgggcgt
     3001 gaggactatc aggaactcga ccgtcgcggc atcagcgtca agggcaggat cgtgatcgcc
     3061 cgctacggca atggctggcg cggactgaag ccgaagctgg cctacgaaca cggcgcagtc
     3121 ggctgcctga tctactccga tccgcacgag gatggctatg gtgccggcga tgtgtatcca
     3181 caaggcggct ggcgtccgcc agatggcgtg cagcgcggct cagtggccga tctgacgcag
     3241 tattccggcg atccgctgac gccgggcgtg ggcgcgaccg ccgatgccaa acggcttgcg
     3301 ctgtcggacg tcaagacgct gatgaaaatt cccgtcatgc cgatctccta tgccgacgcc
     3361 cagccgctgc tggcggcgct cacgggagcg gtggccccgc ctgcctggcg cggttcgctc
     3421 ggccaaacct atcacgtggg tccgggcgca gcccgcgtgc acatgcacgt cgaatcggac
     3481 tggggcatga agaccgtgta cgacgtcatc gcgcgcattg ccggcagcga gctgcccgat
     3541 gagtggatcg tgcgcggcaa tcatcatgat ggctgggtgt tcggagcctg ggacccgctc
     3601 gcaggcaacg cggcgttgct gtcggaagcc aaagcgatcg gagcgctgct gaaaagcggc
     3661 tggcgcccgc ggcgcaccct gatctattgc agctgggatg cggaagagcc cgggttgatc
     3721 ggatcgaccg aatgggccga gacccacgcg ctggagttgc gggctcatgc cgtgctgtac
     3781 gtgaactcgg ataccaatgc acgcgggttt ctggagatgg gcggcagtca ttcgttacag
     3841 cgtctgctca acgaagtggc cgaaggcgta cacgatgcgg agagcgacag cagcgtgctg
     3901 gcgcgactgc gggcgaagct gcgcgtggag gcgtacaacg gcggcaacga gcagatccgc
     3961 aagctcgcgg cggcggccgc ctccggtgcg gatatcccgc tgacggcgct cggctccggc
     4021 tcggactaca cgccgtttct gcagcatctg gcaatcgcca gtgtcgatct gggctttggc
     4081 ggcgaggccg acaatgccgg ggtctatcat tcgcagtacg acagcttcga acactacgag
     4141 cgcttcggcg atccgaagtt cgtgttcggc atagcgctgg cgcaaagtgc gggtcacctg
     4201 atgttgcgca tggcagacgc ggatgtcttg ccgctgcagt tcaccggcat ggccgaggtg
     4261 atggccgact atgtgcacga actgcaccag cttgccgatg aacgacgcga gcacgcacag
     4321 cggctctcgg agctgcgaca gtcccgcgcg ttcgaactgg cgagcgatcc gacccgtccg
     4381 ctggctgcac cggcggcgga gtcggaggtg ccgttcctgg atttcgcgcc gctcgacaat
     4441 gccagcgcgc atctgaggcg cgccgcccgg gcctacgatg aggcctatgc ggaggccagc
     4501 gcgaggggct ttgccgtggg cgcagcgcgc gagaagcagc tggatgagac cttgcggggc
     4561 ctggagcaga cactggccag tgatcagggg ttgcccgatc ggccctggtt ccggcatctg
     4621 atctacgcac cgggcgtgta caccggctac ggcgcgaaga ccatgcccgg agttcgcgag
     4681 gcgatcgagg atcatcgctg ggaggtggcc tcacagtacg tgcacctcac ggccgaggcg
     4741 ctggagcgct acgccgaaca tgtggaggcc gcgaccgcgc tgctgcgagg gtagattttg
     4801 agacggcatc tgttcgagcg gcactatgtc agccgcatca actggcttcg tgcggcggtc
     4861 ctgggcgcga acgatgggat tgtttcggtg gcgagtctgc tggtgggtgt cgccgccacg
     4921 cacgtttcac ggcaacaagc cctgctggtc ggggtcgcgg gcctggtcgc cggtgcgatg
     4981 tcgatgtccg cgggtgagtt cgtctcggtc agttcccagg ccgatgccga acgctcggac
     5041 ctggagcggg aacggcgtga actggaggcc gacgagtcgg ccgagcgcgc ggaactggcc
     5101 gccatctatg tcaaacgcgg attgaaaccc gccctggccc aggaagtggc cgtgcagctg
     5161 atggcgcagg atgcgctggg ggcgcatgcg cgcgatgagt
//
LOCUS       DATMRM010000148        13091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1647722, whole genome shotgun sequence.
ACCESSION   DATMRM010000148 DATMRM010000000
VERSION     DATMRM010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13091
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647722"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1647)
                     /locus_tag="VNZ06_04360"
     CDS             complement(247..1647)
                     /locus_tag="VNZ06_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HWW20015.1"
                     /translation="MRRQRRWIAHPLLRSALLAAALSADALTQAQAAAPAADDSLTWR
                     GITLYGAIDIGLQYESHGAPFSDFYTPASTNIIQKAGREKVTGATGSNESQSKVGLRG
                     REPIVGDWSGVFALETWFNPQAGQLSNSLKSLIVNNGLATDQQKTSSDGASAGQLVQI
                     AYVGIHSDTFGTLTFGRQQTLVADGITALDPNLAAPAFSLLGASGTYAGAGTTEDKRL
                     DSTVKYLVSYADSIHFGALFIFNDSYASAGGTGFQANIGGHYAGLTIDTYYSKIYDAV
                     SATSLSATQVAGLSKLGFTSDNSLSATISDNTTLAIMAAYTIDPIPVKLYAGYERIQY
                     ANPTHPLAAGVSGLGGYTLAFVSNTAYSNDKKLDVYWAGVRYSVLRPLAITAAFYGFR
                     QNAYGTGSQAGCASVAFSTCSGSFRAYSLDADYRFTKRFDIYAGAMYSAVSDGAASGY
                     LDRTNINPTIGARFIF"
     gene            complement(1828..3054)
                     /locus_tag="VNZ06_04365"
     CDS             complement(1828..3054)
                     /locus_tag="VNZ06_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWW20016.1"
                     /translation="MATTLRLAGMNDLDLALVREAENQAKAVNDAATLRFFPWLSAGA
                     GYNKDTGAAQAFGGKVSSVNQQLYERSVAVNLPLDLGNALFEKFAARQLRTAAAYDSE
                     AQHNDTLFAAAGAYFDLVNAVAAEDIATEAVRISQDYEQQLDRGYRAGLINRSELLRV
                     SVQTQRDGVLLRQAQAATRIASAALATRLRLDPVIGLKPTEHLVAPPTLVPVDVAVQT
                     LVKDALAYRPELRASAASVAAADQERIAAKYGPLIPSLAAQAIYGQIRGGADGYLGDY
                     MSAHEFAIGLNWRLGPGGLFDFSRTEGANSQLRHRRLTDAKLHDDIAQQVVDAYEAAR
                     AGLDQMGLARHSVELAEESLKLSEERKEFGVYAVLEVIQAQQDLTQARNDYAQALTQY
                     AKAQYGLARATARIGE"
     gene            complement(3231..4082)
                     /locus_tag="VNZ06_04370"
     CDS             complement(3231..4082)
                     /locus_tag="VNZ06_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWW20017.1"
                     /translation="MFGPLTHSAENPSKAPDATPRNATAPMGTLVLPAEVVAYQSALL
                     TAKVAGYLKRIPVDKGDKVKTGELIADIEVPELLADRVQYTAQVDVARREYERMQQAA
                     KTAPDLVTPDNLDNARGKFEVARAQLDRVNVLLNYARITAPFSGTITARYVDPGAFIP
                     VPSQSSQQSAGIVTLMDFSHVRIQMFVPENDAYQVRPGCKAVFTAVGLPDKRFTAAIT
                     RVSYALDRNVRTMLAEIDMLNPNGLLQPGMYLSVQLTPAPPEAAPANGIPKAYPKSAK
                     PDAAAVR"
     gene            complement(4136..7297)
                     /locus_tag="VNZ06_04375"
     CDS             complement(4136..7297)
                     /locus_tag="VNZ06_04375"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW20018.1"
                     /translation="MNFVQFALRRPYTVLVLVLGVMVGAALGIRAMARDIFPPLGVPT
                     IYVAQPYGGMDPAQMEGFLTYYYEYHFLYITGIEHVESKSIQGAALIKLQFYPGTDMS
                     QAMSETVSYVNRARAFMPPGTVPPFVMRFDAGSVPVGYLAFSSQSRSVGQIQDLALNT
                     VRPLFATLPGVSAPPPFGGSPRAIVVALDASKLQGYGVSPQDVVAAIANAETINPSGN
                     IYLDGKYPVVPVNSIVKDVKDLAAVAVRTGSFPTVFVRDVGQVSDASDIVTSIALVNG
                     RPTVYIPVTKRADASTLSVVDLVRENLPKFQAALPEDVNISYVFDQSPFVTRAIASLT
                     TEGILGAILSGVMVLLFLRDWRSALIVVINIPISLLAAVFALWITSQTVNIMTLGGLV
                     LAVGILVDMSTVVIENIHTHLARNERVEWAVLDAGQEVAVPLAIAMLCVLAVFLPSFA
                     MQGAARALFVPLSLAVGFSMLASYVLANALVPVLSVWAFRARHAANRSEGDIERGFFA
                     RVQQEYMKWLRPLVSYRWLILWTYLLTCGAAVGLLFPRLGTDIFPRVEASQLQLRLRA
                     PAGTQLDLTAAATQRVLKIIADEAGPDAIVLSLGLVGVHGSAYPINFIYQWNSGPQES
                     VLQVQFKQTAHLDMRALRERLRSRLSRETPNLVVSFEPADIVSRVMSFGAATPIEVAV
                     SGPDLRDDRSFADKVRAKLGKLPMLRDVQFGQPLDYPSVSVTVDRERAGVLGFTANDV
                     TRSLTPATSSSRFTQPVYWAAANGVAYQVQVEIPQQLMGSIEDLRNVPVAGDGSHSTL
                     LRNVASVQSGAVVGEYDRYNMARMVTVTANIEGDSLGGAAAAVTDAIAQLGSPPARVS
                     VAIRGEVQPMTQLLGALRVGLLLSILVIFLLLVANFQSWQLSLITIVSVPAVMAGVSV
                     LLWLTRTTLNLESFMGAIMAVGVAVANAILLVTFAERARMSGQSAVEAALSGAASRLR
                     PILMTSLAMIAGMLPMAAGIGESGQQSAPLGRAVIGGLALATLATLFILPAVFALVRA
                     TAGRGSPSLHPDDSPGAPREPAR"
     gene            complement(7294..7476)
                     /locus_tag="VNZ06_04380"
     CDS             complement(7294..7476)
                     /locus_tag="VNZ06_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20019.1"
                     /translation="MMRILIVRDEAKAAALRRGLTEPGFAVAAVGTGEEGLFRAPNLD
                     CELVILEATLTFWDQR"
     gene            complement(7570..9342)
                     /gene="lpdA"
                     /locus_tag="VNZ06_04385"
     CDS             complement(7570..9342)
                     /gene="lpdA"
                     /locus_tag="VNZ06_04385"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWW20020.1"
                     /translation="MSGEVSCPDLGDFKDVAVIDVMVKPGDRIEVETALITLETDKAT
                     MDVPSTAAGKVAEVLVKKGSRVSKGTPLVRIEAADAAAKPTPVVAAAPSPATPATQER
                     KQSPTTPPSATAPAQEPEASRSTQLLVLGSGPGGYSGAFRGADLGLKVTMVERWDVLG
                     GVCLNVGCIPSKALLHAARVIEETHEMASRGIEFGPPTLNFDKLRDWKASVVKKLTGG
                     LTVLAKQRKVELVRGVGKFISPHVLEVTQGEQRERIRFEHCIIAAGSEAIRLPFIPDD
                     PRIIDSTGALELPPNPGRFLVIGGGIIGLEMACVYDALGAKVSVVELTGQLMPGCDAD
                     LVRPLEKRVRARYSEVLLNTKVTAVTPGKEGLQVKFEDKEGNSSTHTYDYVLVAVGRT
                     PNGNNIGAEAAGVKLDQRGFIPTDKQMRTNVPHIFAIGDIAGPPMLAHKATHEAKVAA
                     EVAAGRKSAFDARVIPSVAYTDPEVAWVGLTESEAKAQGITIEKAAFPWVASGRSLSI
                     ARDEGFTKLIADPQTHRILGAGIVGTNAGELIAEAGLAIELGADAADIGLTVHAHPTL
                     SETVAFAAEAIDGTLTDLYLPRKR"
     gene            complement(9339..10694)
                     /locus_tag="VNZ06_04390"
     CDS             complement(9339..10694)
                     /locus_tag="VNZ06_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HWW20021.1"
                     /translation="MASKEIHVPDLGDFKDVAVIDVLVKAGDTVQVETPLLTLETEKA
                     TMDVPSTEAGVIDKMHVSKGSKVSAGTLIATVAAAGGAAEKPAAPPAEKAAPAAKTEA
                     PRAPTPAPTASATPAPVAAKAAASAEVRAGAITGAITGAINEASFAGAHASPSVRRFA
                     RELGVDLGKVAGSGQKGRVTEEDVKAFVKSLMAGAAAPASAAGSGLPRVSVPDFAQFG
                     PIEVRPLNRVQRISGPRLHASWVNLPHVTQFDEADVTDMEKLREELKTRAEAAGIKLT
                     PLAFIVRACVKALQKFPLFNASLDASGENLVYKKYLHVGFAADTPNGLLVPVIRDADR
                     KDIYEIARVLADLSAKARAGKLSAAEMQGGSFTISSLGGIGGTAFTPIINAPEVAILG
                     VSRSGHRPVYRDGSFVPRLMLPLSLSYDHRVVDGANGARFTSYLAAVLADAQGLAEAV
                     P"
     gene            complement(10712..>13091)
                     /gene="aceE"
                     /locus_tag="VNZ06_04395"
     CDS             complement(10712..>13091)
                     /gene="aceE"
                     /locus_tag="VNZ06_04395"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HWW20022.1"
                     /translation="KANKKSTEYGGHLASYASSATLYEVGFNHFWRAPDDNHPGDLVY
                     IQGHSSPGIYGRAFLEGRLTEDQLSHFRQEVGGRGLSSYPHPWLMPEFWQFPTVSMGL
                     GPLMAIYQARFIRYLENRGLIAPSDRKVWTFCGDGEMDEPESMGALTMPVREGLDNLI
                     FVVNCNLQRLDGPVRGNGKIIQELEAAFLGAGWNVIKVIWGSRWDPLLARDTRGILQK
                     VMEECVDGEYQNFKAKGGAYTREYFFGKDPTLKEMVANMSDEDIWRLNRGGHDAQKVY
                     AAYEAATHIKGVPTVILAKTVKGFGLGKTAEGQMVAHQQKKLDIDALKELRDRFNIPV
                     SDQDLTDLAYRKPADDSDEMRYLHARRKQLGGYLPSRKASAPPLTVPPLEAFRAILDG
                     TADREISTTMAFVRILTVLLKDKGIGKNIVPIVPDEARTFGMEGLFRQIGIYSAVGQL
                     YTPVDAETLMSYREDKKGQMLEEGINEAGSACSWIAAATSYANHGISMVPFYIYYSMF
                     GFQRVGDFFWAAGDMRARGFLIGATAGRTTLAGEGLQHQDGHSQLVATTIPNCRTYDP
                     AFAYELAVIIQNGMQRMYVENQGVFYYISVMNENYPQPALPAGSEQGIVNGGYLLQAG
                     GKGKLRATLLGSGTILRECIAAAALLEQTFSIPADVFSITSFSELRREALECERWNLL
                     HPSETPRVAYVRTLLGGREGPLVAATDYVRTVPDQIRSWVTPDYATLGTDGFGRSDAR
                     APLRQHFEVDRNFIALAALKALADSNRIDRRTVVEAIGKLGIDPDKADPLNS"
BASE COUNT         2551 a         4098 c         3952 g         2490 t
ORIGIN      
        1 tcgcgcagcc tgctggggtg ggtggagcat cggcggcgct agtcgcatca ttcacgaaca
       61 tgctcgacac agcgcccccc aggtacagca aacccaccgg cagcagcctc ctggcagcaa
      121 acttggaacg aagcatcggt atctcccggg cgatcattgt tatatagtcg agtatataac
      181 gttaatcgca agctggcagc caccgggagg taagcacccg ctcaggcgcg acgcggtcgg
      241 tatcagttag aaaatgaagc gtgcgccgat agtcggattg atgttggttc ggtcgaggta
      301 gccgctggcc gcgccgtccg acactgcgct gtacatggca ccggcgtaga tatcgaaccg
      361 tttggtgaag cgatagtcag cgtcgagcga atacgcccga aagctgccgc tgcaagtgga
      421 gaacgccacg gaggcacatc cggcctggct accggtgccg taggcgtttt gccggaagcc
      481 gtagaaggcc gctgtgatag ccaacggtcg aagtacgctg taacgcacgc cggcccaata
      541 gacgtctagc ttcttatcat tggaataggc ggtgttgctg acgaaggcca gggtgtagcc
      601 acctagaccg gacacaccgg ccgccagcgg atgggtggga ttggcatatt gaatgcgctc
      661 gtatccagca tagagtttta ccgggatcgg gtcgatcgta taggcggcca tgatcgcgag
      721 cgtcgtgtta tcggagatcg ttgccgacaa ggaattgtca gatgtgaagc ccagcttgga
      781 cagtccagcg acctgcgtcg cgctgagtga ggtcgccgaa accgcatcgt agatcttgga
      841 ataataggta tcgatcgtca gccctgcgta gtggccgccg atattggcct ggaaccccgt
      901 gccgccggct gaggcgtagg aatcattgaa tatgaacaag gctccaaaat gaatgctatc
      961 ggcgtagctg acgaggtatt tgacggtcga atccagacgc ttgtcttcgg tggtaccggc
     1021 acccgcgtag gttccggatg cgccgagtaa cgagaatgcc ggggccgcaa ggttggggtc
     1081 gagcgcagtg atgccgtcgg ccacgagggt ctgctgccgg ccgaacgtca gggtgccgaa
     1141 agtatctgaa tggatgccga cgtaggcaat ctgaaccagc tgaccggcgc tggcaccatc
     1201 gctactggtc ttttgttggt ccgtcgcaag gccattgttc actatcagcg atttcagcga
     1261 attgctcagt tgaccggcct gcgggttgaa ccacgtctcc aacgcgaaca cgccagacca
     1321 atctccgaca atcggttcgc ggcctcgcaa gcccaccttg gactggctct cgttgctgcc
     1381 ggttgcaccc gtcacctttt cgcggccggc cttctggatg atattcgtgc tggccggcgt
     1441 ataaaaatcg ctgaacggcg cgccatgtga ttcgtactgc aagccgatat cgatagctcc
     1501 gtagagcgtg attccacgcc acgtcaggct gtcatcggcc gcaggcgcag cagcctgcgc
     1561 ctgcgtaaga gcgtccgcac tcaacgcggc cgcgagcaga gccgaacgca acaaggggtg
     1621 cgcgatccaa cggcgctgtc gtcgcatgca agcagctcct gtcgtgtgtg agatcgactt
     1681 gtatcagcgt cgatttgtga gcatcgtgac cgcgcggagt gacaatgtca gtctcacggc
     1741 gataacaatg cggttacttg cgtaaccgga cgacatccgt cggattcctc gtggccacca
     1801 gctcagtgtg tcgaaggtag cgtctgctca ttccccgatt cgcgccgtcg cgcgcgccag
     1861 tccgtattgc gctttggcat attgggtcag ggcttgagcg tagtcgtttc gtgcctgcgt
     1921 gaggtcctgt tgcgcctgga tcacctcgag caccgcgtag acgccaaact ccttgcgctc
     1981 ctcgcttagc ttcaggctct cctcggccaa ctcgacgcta tgccttgcca ggcccatctg
     2041 gtccagaccc gcgcgcgctg cctcgtaggc gtccaccact tgttgcgcaa tatcatcgtg
     2101 gagttttgca tctgtcagac gccggtgtcg cagctgtgag ttcgcccctt ccgtgcggct
     2161 gaagtcgaac aggccgccag gccctaaacg ccagttcaag ccaatggcga actcatgcgc
     2221 actcatgtag tcaccgaggt agccgtcggc gccgccacgg atctgaccgt agatggcctg
     2281 cgcggcgaga gaaggaatca atggaccgta cttggcggca atgcgctcct ggtctgcagc
     2341 tgcgacgctt gcggcgctcg ccctgagttc ggggcggtac gcaagcgcgt ctttgacaag
     2401 cgtttggact gcgacatcga ccggcaccag cgtcggaggc gcaacgaggt gctccgtggg
     2461 cttaaggccg atgacaggat ccaagcgaag ccgcgtggca agcgctgctg aagcgatgcg
     2521 cgtggccgct tgcgcctgtc gcaaaagaac cccatcgcgc tgcgtctgca cgctgacgcg
     2581 caagagctca ctgcgattga tcagcccagc gcgatagcca cgatcgagct gctgctcgta
     2641 gtcctgagag atgcgaactg cctcagtcgc aatgtcttcg gcagccaccg cattgaccag
     2701 atcaaagtac gcgcctgcag cagcgaacag ggtatcattg tgttgcgcct cgctatcgta
     2761 cgcggccgcg gttcgtaact gtcgggcggc aaacttttca aacagcgcgt tgccaagatc
     2821 cagcggcagg ttcacggcca ctgaacgctc gtagagctgc tggttgaccg aggacacctt
     2881 tccgccaaag gcctgcgcag cacctgtgtc tttgttgtag ccagctccag cgctgagcca
     2941 cggaaagaat ctcagtgttg cggcatcatt gacggctttg gcctgattct ccgcttcacg
     3001 gaccagcgcc agatcgagat cattcatccc ggcaagacgc agcgtcgtcg ccaagtcgat
     3061 cgtttggcgg tcatccggct gtgctggatc cgtcctaacg acgatggggg cgttcgcggc
     3121 tgggttgggc gtgtcgcccg gttgctgcgc gagagctcgg actccaacga gtaccagtgg
     3181 cagcgctccg acgatagccc caaccgggcg gcgccgaacg acacgaggtt tcatcgcacc
     3241 gccgcggcgt ccggcttcgc cgacttcgga taggctttgg gaattccatt tgcaggtgct
     3301 gcctcgggcg gcgcgggtgt caactgtacc gatagataca tgcccggctg cagcagcccg
     3361 ttcgggttca gcatgtcgat ctcggcgagc atcgtgcgaa cgttccgatc cagcgcgtaa
     3421 ctgacgcgcg taatggcggc tgtgaagcgc ttgtctggca ggccgacggc ggtgaacacg
     3481 gccttgcaac ccggtcgaac ctggtaggcg tcgttctcag gtacaaacat ctggatgcgt
     3541 acgtggctga aatccatcag ggtcacgatg ccggcgcttt gctggctgct ctggctcggc
     3601 accggaatga atgcgcccgg gtcaacgtaa cgcgccgtga tcgtgcccga gaagggagct
     3661 gtaatgcgtg cgtaattgag cagaacgttc acacgatcga gctgagcccg cgcgacctcg
     3721 aatttccccc gtgcattgtc gagattgtcc ggagtcacca gatccggcgc cgtcttggcc
     3781 gcctgctgca tgcgctcata ttcgcgacgt gcgacatcca cttgggccgt atactgcacg
     3841 cggtccgcca gtagttctgg tacctcaata tctgcgatga gctcaccggt cttgaccttg
     3901 tcgcctttgt ccaccgggat gcgcttgagg tagccggcga ccttcgccgt gagcaacgcg
     3961 gactgatagg ccacgacctc cgccggcagt accaaggtgc ccatcggggc cgtagcgttg
     4021 cgcggtgtcg cgtcgggcgc tttcgatgga ttctccgctg agtgagtcag cggcccgaac
     4081 accgcacaga gtatgagtgc aatggccgcg tggcatctac gtgggttggc gaacgtcatc
     4141 gtgcgggttc cctcggggct cctggagaat catcgggatg cagcgaaggt gatccacggc
     4201 cagccgttgc acgcactagc gcaaagactg ctggcaggat gaagagcgtc gcgagcgtcg
     4261 ccagtgcaag ccctccgatc actgcccggc cgagcggtgc gctttgctgt ccgctctcgc
     4321 cgatgcccgc tgccatcggc aacatgccgg caatcatggc gaggcttgtc atgagaatcg
     4381 gtcgcaagcg gcttgcagcc ccggacaatg cagcctccac cgcactctgt cccgacatgc
     4441 gtgcgcgctc ggcgaaagtg accagcaaaa tggcgttcgc aaccgctacg ccgacggcca
     4501 tgatcgcccc catgaaggac tccagattga gggtggttcg cgtgagccac agtaggaccg
     4561 atacaccagc catgacggcc ggcacgctca caatcgtaat gagcgagagc tgccaggact
     4621 gaaagttcgc aaccaggagc aggaaaatca ccagtattga aagcagcagg cccacgcgaa
     4681 gcgcgccgag gagttgcgtc atgggctgaa cttcgccgcg aatcgcgacg cttacccttg
     4741 ccggaggaga cccaagctgc gcaatcgcat cggtaaccgc tgcagccgcg ccgcccaggc
     4801 tgtcaccctc gatattggca gtcactgtta ccatgcgcgc catgttgtag cgatcatatt
     4861 cacccacgac tgctccggac tgaacgctcg ccacattgcg cagcagagtc gaatgactgc
     4921 catcacccgc gaccggtacg ttgcggaggt cctctatcga acccatgagt tgctgtggaa
     4981 tctcgacttg gacctgatag gccacgccat tggccgcagc ccagtagaca ggctgggtga
     5041 agcggctaga ggaggtcgcc ggggtgagcg agcgggtcac atcgtttgca gtgaagccga
     5101 gcactccggc gcgctcccgg tcaaccgtga cgctgacaga tggatagtca aggggctgtc
     5161 cgaactgaac gtcgcgcagc attggcagct tccccaactt cgcgcgcacc ttgtccgcga
     5221 agctgcgatc gtccctgagg tccggaccgc tcacggcgac ctcaatgggc gttgccgcac
     5281 cgaaactcat cactcggctc acaatgtcgg ccggctcaaa cgagactacc aggttcggcg
     5341 tctcgcgcga caagcggcta cgcaaccgct cgcgcaatgc tctcatatcc agatgggccg
     5401 tctgcttgaa ttgcacttgc agcacgctct cctgcggtcc gctattccac tgataaatga
     5461 aattgatcgg gtatgcggag ccatgcacac ccaccaggcc gagcgacagt acaatcgcat
     5521 ctggcccggc ctcatcggcg atgattttca gtacccgctg agtggcggcc gcggtgaggt
     5581 ctagctgggt ccctgcagga gcgcgtaagc gcagttgcag ctgactggct tccacgcgcg
     5641 ggaagatatc cgtgccaaga cgcggaaaca gcaagccgac tgcggctccg catgtcagca
     5701 gatacgtcca cagaatcaac cagcggtagc tcaccagcgg ccgaagccac ttcatgtact
     5761 cctgctgcac ccgtgcgaag aacccacgtt cgatgtcgcc ctcactcctg ttagcagcgt
     5821 ggcgagcgcg aaacgcccaa accgaaagca cgggcacgag tgcattggcc agaacatagg
     5881 aggccaacat cgagaaaccg accgccagcg acaggggcac gaacagtgct ctggcggcgc
     5941 cctgcattgc aaaggaaggc aggaacaccg caagcacaca cagcattgcg atcgccagcg
     6001 gcacggcgac ctcctggccg gcatccagta ccgcccactc aacgcgctca ttgcgtgcaa
     6061 gatgcgtatg aatgttctcg ataaccacgg tcgacatgtc caccaatatg ccgacggcaa
     6121 gcacgagccc gcccagcgtc ataatgttta ccgtctggct ggtgatccat agcgcaaata
     6181 cggcggccag cagtgaaatc gggatgttaa tgacgacgat gagcgcgctg cgccagtcgc
     6241 gcaggaacag aagcaccatg acacctgaga ggatggcgcc cagtatgcct tctgtcgtca
     6301 ggctggcaat ggcgcgagtg acgaaaggtg attgatcaaa aacgtagctg atgttcacat
     6361 cttcgggcag agccgcctga aactttggta ggttctcccg gacgaggtct accaccgata
     6421 gcgttgatgc atcggcacgc tttgtgacag ggatatagac cgtgggcctg ccgttgacga
     6481 gcgcaatact cgtcacaatg tccgaggcgt cggacacttg gcccacgtct cgaacgaaca
     6541 ccgttggaaa actgcctgta cgcacggcca cggcggccag gtccttcacg tccttcacga
     6601 ttgaattcac cggaaccacc gggtacttcc cgtcaagata gatgttcccg gaagggttga
     6661 tggtctcggc attggcgatt gctgccacga cgtcttgcgg tgagacgccg taaccttgaa
     6721 gcttgctcgc atccaaggcg acgacgatgg cgcgcgggct gccaccaaaa ggcggcgggg
     6781 ccgagacacc gggcaacgtt gcgaacagcg gtcgcaccgt gttgagcgcg agatcctgaa
     6841 tctgtcccac gctgcggctc tggctggaaa atgcgagata gccgactggg acgctccccg
     6901 catcgaagcg catcacaaac ggcggcaccg tacccggcgg catgaaggca cgcgcgcgat
     6961 tgacgtagga gaccgtctcg gacatcgcct gggacatgtc cgtgccagga tagaactgaa
     7021 gcttgatcag tgccgcgccc tggatcgact tcgactcgac gtgctcaata ccagtgatgt
     7081 aaaggaagtg gtactcatag tagtaggtga ggaaaccttc catttgcgcc ggatccatac
     7141 cgccataggg ttgcgcgaca tagatcgtag gcacgccgag cgggggaaag atgtcgcgtg
     7201 ccatggcgcg aatgccgagc gcggcaccga ccatgacccc aagcactagc accagcacgg
     7261 tgtacggtcg gcgcagtgca aactgcacga agttcatcgc tgatcccaga aggtgagcgt
     7321 ggcctcgagg atgacaagct cgcagtcaag attgggtgct cggaagagtc cctcttcgcc
     7381 ggtgccaacc gcagccacgg caaatcctgg ctcggtgagg ccccggcgca atgcggctgc
     7441 cttcgcctcg tcccgcacga tcaaaattcg catcacatca gcaactcctc gtcccgcgcg
     7501 aagcgcagcc catcatagcc ggtacgcagg actgcacgga gtgcagcacc cacgcggagc
     7561 gatcggggct tagcgcttcc tcggcaggta cagatccgtc agcgtgccgt cgatcgcctc
     7621 ggcggcaaat gccaccgtct cggacagggt tgggtgggca tgaacggtga gcccgatgtc
     7681 cgcggcgtcg gcgcccagct cgatcgcaag cccggcttct gcaatcaatt caccggcatt
     7741 ggtgccgacg ataccggctc caaggatgcg gtgcgtctgc ggatcggcga tcagtttcgt
     7801 gaaaccctcg tcgcgagcga tggatagtga acgcccactg gcgacccacg gaaacgcggc
     7861 tttctcgatc gtgatgccct gcgctttcgc ctcgctttca gtcaggccga cccatgcaac
     7921 ttccggatct gtataggcca cggaaggaat cacgcgcgca tcgaaagcgc tcttgcgccc
     7981 cgcggccact tcggcagcaa ccttcgcctc atgagtggct ttgtgcgcca gcatcggcgg
     8041 cccggcgatg tcgccgatcg caaagatgtg tggcacgttg gtccgcattt gcttgtcggt
     8101 cggaatgaaa ccgcgctgat ccagcttcac gccagcggcc tcagcgccaa tgttgttccc
     8161 attgggcgtg cgcccgacgg ccaccagcac atagtcatag gtatgggtac tgctattgcc
     8221 ttctttgtct tcgaacttca cctgcaggcc ttccttgccg ggagtgaccg cggtgacttt
     8281 ggtattgagc agcacttcgc tgtacctggc tctgacgcgc ttttcgagcg ggcgcaccag
     8341 atccgcgtca cagccaggca tcaactgccc agtcagctcg accacgctga ccttcgcacc
     8401 cagtgcatcg tagacgcacg ccatctccag accgatgatg ccaccgccga tcacgaggaa
     8461 gcggccgggg ttcggcggca gctcgagtgc gccggtcgaa tcgatgatgc gtgggtcgtc
     8521 ggggatgaat ggcagacgga tcgcctccga accggccgca atgatgcagt gctcgaagcg
     8581 gattcgttcc cgctgctcgc cctgcgtgac ctccagcacg tgcgggctga tgaatttgcc
     8641 cacgccccgc accagctcga ccttgcgctg tttcgccaac acggtgagac cgccggtgag
     8701 ctttttcacg acgctggcct tccagtcgcg cagtttgtcg aagttcagtg tcggcggacc
     8761 gaattcgatg ccgcgcgaag ccatctcatg cgtctcttcg atcactctcg cggcgtgcag
     8821 cagtgctttg gatgggatgc aaccgacgtt cagacacacg ccgccgagca cgtcccagcg
     8881 ctcgaccatg gttaccttca gacccagatc tgcgcctcga aaggcacctg aataaccccc
     8941 gggtccggaa cccagtacga gcagttgcgt gctgcgcgag gcctccggct cctgcgcggg
     9001 cgcggtcgca gagggcggtg tcgtcggcga ctgcttgcgc tcttgcgtcg ccggtgtggc
     9061 cggggaaggc gcagctgcca ctaccggcgt cggtttcgcg gcggcgtccg ccgcttcgat
     9121 gcggaccagc ggcgttcctt tggagacacg gctacccttc ttgaccagca cctcggcgac
     9181 tttgccggcc gccgtggacg gcacgtccat cgtcgccttg tcagtttcca gggtaatcag
     9241 agcggtttcg acttcaatcc gatcgccggg tttgaccatt acgtcaatga ccgcgacatc
     9301 cttgaagtct ccgaggtcgg ggcacgagac ctccccgctc acggcaccgc ctcagccagc
     9361 ccttgtgcat cggcgagcac agcagccaga tagctggtga agcgtgcgcc gttggcgcca
     9421 tcgaccacgc gatgatcgta cgacaatgaa agtggcagca tcagtcgcgg tacgaagctg
     9481 ccgtcgcgat acaccggcct gtggcccgag cgcgagacac ccagaatggc gacctcgggt
     9541 gcattgatga tcggagtgaa cgcggtgcct ccgataccac cgagactcga gatggtgaag
     9601 ctgccaccct gcatttcggc cgcgctgagt ttgccggcgc gcgctttggc ggacagatcg
     9661 gccagcacgc gcgcgatctc atagatgtct tttcggtcgg cgtcgcgaat cacaggcacc
     9721 agcaagccgt taggcgtgtc ggcagcgaag cccacgtgaa ggtatttttt atagaccagg
     9781 ttctcgccgc tcgcatcgag tgacgcattg aacagcggaa acttctgcag tgccttgacg
     9841 cacgcgcgca cgatgaacgc cagcggtgtg agcttgatgc ccgcagcttc ggcgcgggtc
     9901 ttcagctcct cacgcagttt ctccatgtcg gtgacgtccg cctcatcgaa ctgcgtcacg
     9961 tgcggcaggt tcacccaact ggcatgcagg cgcggcccgg aaatgcgctg cacgcgattc
    10021 agtggccgga cctcgatcgg accaaactgc gcgaaatcgg gcacgctgac tcttggcaga
    10081 ccactgccgg cagcgctggc gggcgcggca gcccccgcca tcagcgactt cacaaacgcc
    10141 ttaacgtcct cttcagtgac acggcccttc tgtccgctgc ctgcgacctt ccccagatcc
    10201 acacccagtt cgcgggcaaa tcgtcgcacc gaggggctcg catgcgcgcc cgcaaagctc
    10261 gcctcgttga tcgcaccggt gatcgcaccg gtgatcgcac cggctcgaac ttcagcactc
    10321 gcggcggcct tggccgcaac gggggccggg gtggcggacg cagtcggtgc aggcgtcggt
    10381 gcacggggag cctcagtctt cgcagcgggc gctgcttttt ccgcaggcgg cgcggcaggc
    10441 ttttccgccg cgccaccggc cgctgctacg gtcgcgatca gagttccggc cgaaaccttt
    10501 gagcccttcg agacgtgcat cttatcgatg acaccggctt cggtcgaagg cacatccatc
    10561 gtcgcttttt ctgtttccag cgtcagcaat ggcgtctcaa cttgcaccgt gtcgccggcc
    10621 ttgaccagca cgtcgatgac agcaacgtcc ttgaaatcgc ccaggtcggg aacgtgaatt
    10681 tctttggagg ccaaggcgct tctctgcacg ctcaactgtt gagagggtca gctttgtccg
    10741 gatcgatccc cagcttcccg atcgcctcga ccacggtcct gcgatcgatg cgatttgagt
    10801 cggccagcgc cttcagcgca gccagcgcaa tgaaattgcg atctacctcg aagtgctgac
    10861 gcaacggcgc gcgagcgtcg ctgcgaccaa atccgtccgt gccgagcgtc gcgtagtcgg
    10921 gtgtcaccca ggatcgaatc tgatcgggca ccgtccgtac atagtcagtg gctgcgacca
    10981 gaggtccctc gcgcccgccc agcagcgtgc gcacgtacgc aacgcgcggc gtctccgacg
    11041 gatgcagcaa gttccagcgc tcacattcga gcgcttcgcg ccgcagctca ctgaagctcg
    11101 tgatcgaaaa cacatcggca ggaatactga acgtctgctc cagcagcgcg gccgccgcaa
    11161 tgcattcgcg caaaatagtg ccgctgccga gcagcgtcgc cctgagcttg cccttgccgc
    11221 ctgcctgcaa caggtagccg ccgttcacga tgccctgttc cgaaccggcc ggcagtgccg
    11281 gctgcggata gttctcattc atgaccgaga tgtaatagaa cacgccctga ttctcgacgt
    11341 acatgcgttg catgccgttc tggatgatca cggcaagctc ataggcaaaa gcgggatcat
    11401 aggtgcggca gttaggaatc gtggtcgcca cgagctgact gtgtccgtcc tggtgctgca
    11461 gaccttcgcc cgcgagcgtg gtgcgaccgg cggtggcacc gatcaggaat ccgcgcgcgc
    11521 gcatgtcgcc agcggcccag aaaaaatccc cgacgcgctg gaagccgaac atcgagtagt
    11581 agatatagaa cggcaccatg ctgatgccgt gattggcgta ggaggtggcg gccgcaatcc
    11641 acgaacaggc ggagccggct tcgttgatac cttcctccag catctgacct ttcttgtcct
    11701 cgcggtagga catcagtgtc tcagcatcaa ccggcgtata gagctgaccg accgcggagt
    11761 aaattccgat ctgacgaaac aggccttcca taccgaaggt gcgcgcctca tccggcacga
    11821 tcggtacgat gttcttgccg atgcccttgt ccttcaacag cacggtcaga atgcgcacaa
    11881 acgccatcgt ggtagagatc tcgcggtcag cagtaccgtc gagaatcgct cggaacgcct
    11941 ccagtggagg caccgtcaac ggcggtgcgc tcgccttacg ggatggcaga tagccgccca
    12001 gttgcttgcg gcgtgcgtgc agatagcgca tctcatcgct gtcatccgca ggcttgcgat
    12061 acgccagatc agtcaggtcc tgatcggaga cgggaatgtt gaaccggtcg cgcagctcct
    12121 tgagcgcgtc gatgtcgagc ttcttctgct gatgggccac catctgaccc tcagccgtct
    12181 tgcccagacc aaatcccttg acggtcttcg ccaggatgac ggtcggtacg cccttgatgt
    12241 gggtggccgc ctcgtaggcg gcgtacacct tttgagcatc gtgaccaccg cgattgagac
    12301 gccagatgtc ctcatcggac atgttggcga ccatctcctt caacgtcgga tccttgccga
    12361 agaaatattc gcgcgtgtac gccccacctt tggccttgaa gttctggtac tcgccgtcga
    12421 cgcattcttc catcaccttt tgaagaatgc cgcgcgtgtc acgcgccagc agcggatccc
    12481 agcgcgaccc ccagatgact ttgatgacgt tccagccggc tcccagaaac gcagcttcca
    12541 gctcctggat gatcttgccg ttgccgcgta ccggaccatc cagacgctgc agattgcaat
    12601 tcacgacgaa gatcagattg tcgagcccct cgcgcaccgg catcgtcaat gcgcccatcg
    12661 actcgggctc gtccatctcg ccgtcgccgc agaacgtcca taccttgcga tccgagggtg
    12721 cgatcagtcc gcggttctcc agatagcgga tgaaacgcgc ctgatagatc gccatcagcg
    12781 gacccaagcc catcgacacc gtggggaact gccagaactc gggcatcagc cacggatgcg
    12841 gataggaact gaggccccgg ccgcccacct cctgacggaa atgactgagc tggtcctcgg
    12901 tcagacgccc ctccagaaac gcgcggccgt agatgccggg cgaagagtgc ccctggatgt
    12961 aaacgagatc ccccggatga ttgtcatccg gcgcgcgcca aaaatggttg aacccgacct
    13021 cgtatagggt ggccgaggag gcatagctcg ccagatgccc gccgtactcg gtggacttct
    13081 tattcgcctt g
//
LOCUS       DATMRM010000149         4217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4114137, whole genome shotgun sequence.
ACCESSION   DATMRM010000149 DATMRM010000000
VERSION     DATMRM010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4114137"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VNZ06_04400"
     CDS             complement(<1..940)
                     /locus_tag="VNZ06_04400"
                     /inference="COORDINATES: protein motif:HMM:NF036554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HWW20023.1"
                     /translation="MLTALTLLTVLTRPALADIKIDIDGVDGDIRRNVLALLSLERYK
                     DRDRIEADAVQRLYNRIDDEVRSALRPFGYYEPIVTDSIQPPDAQRHWHVRISIDPGA
                     PVLLDTVSVKIDGPGAQDPVFKTALEHPPQRGERLSHSAYDQFKGALERAAATYGYLD
                     ARLVRSQLQVNLTTHKADIYLQLDTGERYRFGKTTIDQTAVRDHEVRRYLRYKEGDPY
                     DALQLLRTQFALDDSLYYSTVEVLAGDHDPATHTVPITVHANNARRGYSFGVGYGTDT
                     GPRGTATWTVPRVNSLGHRFRVQLELSEIEQLFDVRY"
     gene            complement(982..1890)
                     /gene="nudC"
                     /locus_tag="VNZ06_04405"
     CDS             complement(982..1890)
                     /gene="nudC"
                     /locus_tag="VNZ06_04405"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HWW20024.1"
                     /translation="MSASPNSEPPTSTLERRSEARLNPDWARDAARDPETRYLVTRGT
                     THLVSPQAPTQIAFLTAEHPVIARRPESELSLLGWFEGHRIVLVDLPPELQLEVPGTE
                     FQELRPLLAVLPASEIELLSLARALVIWRSRHRHCGVCGASNEPRSAGHVLRCTNAAC
                     GSEFFPRIDPAIIVLVTDGDHALLGRQKSWPKGRYSALAGFAEPGESLEQTVAREVEE
                     ETGTRVSWVRYIASQAWPFPASLMLGFHAGATRGPVRLDGELEDARWMSLQQLQSQAA
                     LLLPPIFTIARRLIEAWMLEQTRTKS"
     gene            complement(1817..2476)
                     /locus_tag="VNZ06_04410"
     CDS             complement(1817..2476)
                     /locus_tag="VNZ06_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20025.1"
                     /translation="MMRISDDRYSRDRSRFDVAMQFIRNEARTHTIRVWTGLSDDRIR
                     KLYRSYLCDARAKPLIRHRGKSPQQAGFFMRSTRSRQEAALLASLCCLVGALPGGPAP
                     ELLGTLPSLGRGELLCHAYGVYRSLIPETMISFEHAVFLLTALVRGDELVLSACRECS
                     AVLIIDRWSLRAPRCALCADDSLAAPRRGLQDLSPDERITEFGAADIHAGASLRSTAE
                     S"
     gene            2590..3240
                     /locus_tag="VNZ06_04415"
     CDS             2590..3240
                     /locus_tag="VNZ06_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20026.1"
                     /translation="MSAYLPPPTQLGNWDDALLAPVAEVNDQMLELLKSMAAAPAMSQ
                     PRVTPRLVQLLREHWRRLDARAQRRLSACPYLLLDADFAQTARWQRLLSAGVMDAPAR
                     GGYFSGPSGVSLIRRMLLLAWHLARSNRLMAAVTLGLTAPVAERIAATPLKDLEAIAE
                     LAPAWIAPRWEQQTRVWQQLITAACSEHPLALRQAQLRGLQLLAREHTRADGGAVC"
     gene            3281..4204
                     /locus_tag="VNZ06_04420"
     CDS             3281..4204
                     /locus_tag="VNZ06_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWW20027.1"
                     /translation="MLALSTRALTKTYNNGVHALRGVELDVEEGDFFALLGPNGAGKT
                     TLIGIVTSLVNKSGGTAAVFGYDIDRSLERAKSCIGVVPQELNFNQFETPMTIVVNQA
                     GFYGIPRAEARVRAEKYLKQLQLWDKRNSTARGLSGGMKRRLMIARALMHEPRLLILD
                     EPTAGVDIEIRRSMWEFLRGINASGTTIILTTHYLEEAESLCRNIAIIDKGNIVERDR
                     MGNLLLRLHTETFVLNTRKPVSSAPSVSEFAVRLVDEHTLEVDISKDQNLNELFSRLT
                     VLGIDVLSMRNKVNRLEEIFMRLVENRGKNA"
BASE COUNT          786 a         1284 c         1383 g          764 t
ORIGIN      
        1 cgtagcggac gtcgaacagc tgctcgattt cagacagttc gagctggacg cggaagcgat
       61 gacccagact gttgacgcgc ggaacggtcc aggtggcggt gccgcgcggg ccggtgtcgg
      121 tgccgtagcc gacgccgaac gaatagccac ggcgcgcgtt gttggcatgc accgtgatcg
      181 gcaccgtgtg cgtcgccggg tcgtggtccc cggcgagtac ttcgaccgtt gaataataaa
      241 gactatcgtc gagtgcgaac tgagtacgca gcagctgcag cgcgtcgtac gggtcgcctt
      301 ccttgtagcg cagatagcgg cgaacctcgt ggtcgcgtac cgcagtctga tcgatcgtgg
      361 ttttgccgaa gcgataacgc tcgccggtgt cgagctgcag atagatatcg gccttgtggg
      421 tcgtcaggtt gacctgcagc tggctgcgca ccaggcgtgc atcgagatac ccgtaggttg
      481 cggcggcgcg ttccagagcc cccttgaact ggtcgtaggc cgagtgactg aggcgctcac
      541 cgcgttgtgg cgggtgttcg agcgccgtct tgaataccgg atcctgggct cccgggccgt
      601 cgatcttgac gctgacggtg tcgagcagga ccggcgcgcc cggatcgatc gaaatcctca
      661 catgccagtg gcgctgggca tcgggcggct gaatcgagtc ggtaacgatg ggttcgtagt
      721 agccgaacgg acgcagcgcc gagcgcactt catcgtcgat tcggttgtac agccgctgta
      781 cggcgtcggc ttcgatgcga tcgcggtcct tgtagcgctc gaggctcagc agtgccagca
      841 cgttgcggcg gatgtcgccg tcgactccat cgatgtcgat tttaatgtca gccagcgcgg
      901 gtcgcgtcag cacggtcagc agggtcagcg cggtcagcaa ccacgtcgcg cgacatggcc
      961 tggaacaggg tcgatggttg ctcaagactt ggtgcgcgtc tgctccagca tccaggcttc
     1021 aatcagccgt cgggcgatcg tgaagatggg tggcaacaat agggctgctt gcgactgcag
     1081 ctgctgcaaa ctcatccagc gggcgtcctc cagttcgccg tccagccgca ccgggccgcg
     1141 ggttgcgccg gcgtgaaaac cgagcatgag cgacgccggg aagggccacg cctgggaggc
     1201 gatatagcgt acccaggaaa cgcgcgtgcc ggtctcctcc tcgacctcgc gcgccacggt
     1261 ttgctcgagg ctctctccag gctctgcgaa gccggcgagc gccgaatagc gtcctttggg
     1321 ccagctcttc tggcgcccga gcaacgcgtg atccccgtcg gtcaccagca cgatgatcgc
     1381 cgggtcgatc cgggggaaga attcggaccc acaggcagca ttggtgcatc gcaatacatg
     1441 gccggcgctc cgcggttcgt tgctggctcc acacacaccg cagtggcggt gacgcgagcg
     1501 ccagatcacc agcgcgcgcg cgaggctcag cagttcaatc tcgctcgccg gcaggaccgc
     1561 tagaagcggc cgcagttcct gaaattccgt acccgggacc tccagctgca gctccggagg
     1621 cagatcgacc agcacgatcc gatggccctc gaaccacccg agcaacgaca gctccgactc
     1681 cggccgcctg gcgatgaccg gatgctcagc cgtcaggaaa gcgatctgcg tcggggcttg
     1741 cggtgacacc aggtgagtgg tgccgcgggt gaccagatag cgggtctcgg ggtcacgtgc
     1801 ggcgtcgcgc gcccagtcag gattcagccg tgcttcggag cgacgctcca gcgtggatgt
     1861 cggcggctcc gaattcggtg atgcgctcat cagggctcaa gtcttgcaga ccgcgcctcg
     1921 gtgcggcaag ggaatcgtcg gcgcacagcg cacagcgcgg tgctctgagc gaccagcgat
     1981 cgatgatcag cactgcagag cactcacggc acgcgctcag caccagctcg tccccacgca
     2041 ccagcgcggt caacagaaat accgcgtgct cgaacgagat catcgtttcc gggatcaacg
     2101 agcggtatac cccataggca tgacacaaca gctcaccccg gccgagggag ggcagcgtac
     2161 cgagcaactc cggggccggg ccgccgggta aggcgccgac caggcagcac aggctcgcga
     2221 gcagcgccgc ttcctgtcgc gagcgtgtgc tgcgcataaa gaagccagcc tgctgtggcg
     2281 acttgccgcg atgtcggatc aagggcttgg cgcgcgcgtc gcataagtag gatcggtaga
     2341 gcttgcggat gcgatcgtcg ctcagcccgg tccacacacg gatcgtgtgc gtgcgagctt
     2401 cgttgcgaat gaactgcatg gcgacgtcga agcgcgatcg atcgcgactg tagcggtcgt
     2461 ccgaaatccg catcatattt ctcccaagta tgttaccagt tacaaattcg gtgcaaatat
     2521 ggcagatatg gcggtgagct ccaaggggca agcgcctcac tatatttccc gggattctgg
     2581 aggaatgcga tgtcagctta cttgccaccg ccgacgcagc tcggcaactg ggatgatgcc
     2641 ttgctggccc cggtcgccga agtgaacgat cagatgctcg agttgctcaa gtcgatggcg
     2701 gctgcacccg cgatgtcgca gccgcgcgtg acgccgcgat tggtccagct gctgcgcgag
     2761 cactggcggc gcctggatgc cagggcgcag cgacgcctgt cggcttgtcc ctatctgctg
     2821 ctcgatgcgg acttcgcgca aacggcgcgc tggcagcggc tgttgtcagc gggcgtcatg
     2881 gacgcgccag cgcgtggtgg ctacttcagc gggccctccg gcgtcagcct gatacgccgc
     2941 atgctgctgc tggcctggca tttggcgcgc tccaatcgcc tgatggcggc tgtgaccctg
     3001 gggctgactg cgccggttgc tgaacgcatc gcggcgacgc cgctgaagga tctggaagcc
     3061 atcgcggaac tggccccggc gtggattgca ccgcgctggg aacaacagac cagggtctgg
     3121 caacagctca tcaccgcagc ctgcagcgag catccgctgg cattgcgtca ggcgcagctg
     3181 cgcggactgc aactgctggc gcgtgaacat acacgcgctg acgggggcgc cgtatgctaa
     3241 gttgcgaggt ttcaagtgat aaggaccggt actgcgagct atgcttgcgc tttcaacccg
     3301 cgccctcacc aagacataca acaacggggt acacgctctg cgtggggtcg aactggacgt
     3361 cgaagaaggg gatttctttg cgctgctcgg ccccaatggc gctggcaaaa cgactctgat
     3421 aggaatcgtc acctcgctgg tcaacaagtc cggcggcacg gcggcggttt tcggctacga
     3481 catcgaccgc tcgctggagc gtgcgaagtc gtgtatcggc gtggtgcctc aggagctcaa
     3541 tttcaatcag ttcgagacgc cgatgaccat cgtcgtcaac caggcaggct tctacggtat
     3601 tccgcgcgct gaagcgcgcg tgcgagcgga aaagtatctg aagcagctac agctgtggga
     3661 caagcgcaat agcacagccc gcggactgtc cggcggcatg aagcgccggc tgatgattgc
     3721 ccgagccctg atgcacgaac cgcggctgtt gattctggat gaacccaccg ctggcgttga
     3781 tatcgagatc cgccgctcca tgtgggaatt cctgcgcggg atcaatgcga gcggcaccac
     3841 catcattctg acgacgcact atctggaaga agccgagagt ctgtgccgca acattgcgat
     3901 catcgacaag ggcaatatcg tcgagcgcga tcggatgggc aatctgctgc tgcgattgca
     3961 caccgaaacc ttcgtgctca acacgcgcaa gccggtcagt tcggctccga gtgtctcgga
     4021 gttcgcggta cggctggtcg atgagcacac cctggaggtc gacatatcca aggatcagaa
     4081 cctgaacgag ttgttttcgc gcctgacggt gctcggcatc gatgtgctga gcatgcgcaa
     4141 caaggtcaac cggctcgagg aaattttcat gcgtctggtg gaaaaccggg gcaagaatgc
     4201 ttgagcgcag caccgtc
//
LOCUS       DATMRM010000150         2330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2562221, whole genome shotgun sequence.
ACCESSION   DATMRM010000150 DATMRM010000000
VERSION     DATMRM010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2562221"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1980)
                     /locus_tag="VNZ06_04425"
     CDS             complement(<1..1980)
                     /locus_tag="VNZ06_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWW20028.1"
                     /translation="MNTPALGRPATASSSLEPYAQLLRALLPRMDTLSVFNERGELYW
                     SSEGVGPELSGIVPLSIKTAEAEPVASGEQRFARGEAVYLFWLRRDDGSRPATPFAVV
                     SLNFRPGNGEHEQRPFSFVQALVKPAIECLRRELLARDEILNLQSSMLEQDNDLEMLL
                     SVSGGGDSKAENSNDLKAIVQSATEHLRVGLSALIVPEKGIAMVQVSADAPLETSLLA
                     KAHRHLLSMAHVRRQATIVNRMVLQQGQNCAAYRVLACPIARPDGRAMGVLALFRAES
                     APEFTAHHGRLTELLARRVASIVTHSYDALTGLLTRPAFEQRVRATLDEDRTAKGQGF
                     KRAWSALFIDINRLHVINDNYGMPMGDRVIAQLGELIRTRLPPGGAIAARISGDRFAI
                     LLPAKLEDAAKFAEALRSGAEEVGAAFGDGKMQVSISIGVSAVDPKSNELVHAFAAAE
                     TACKAANDRGRNRVEVYQETDESIVRRFTDINLIADLRGAIADNRLELNAQLIVPLGG
                     HRGPPHFEVLLRMVGENGEIVGPDHFMSAAHRYQLMPTLDRWVIARSIEILKPHAEVL
                     SASQIVFNINFSGQSLQDAEFTEYVVKLVEDSALNPGSLCFELTESAAIGNLSRAEPL
                     MRRLRKLGCHIALDDFGTGLSSLVYLRTLPVDLLKI"
     gene            complement(2096..>2330)
                     /locus_tag="VNZ06_04430"
     CDS             complement(2096..>2330)
                     /locus_tag="VNZ06_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWW20029.1"
                     /translation="PGLGLLVIIDHGEGYMSLYGHNDRLLKAAGDPVAGGEAVAAAGD
                     TGGRASPELYFEIRRAGKPVDPTPWFRTSSPPP"
BASE COUNT          421 a          766 c          718 g          425 t
ORIGIN      
        1 gatcttcagc agatcgaccg gcagcgtgcg caggtagacc agcgaactca gaccggtgcc
       61 gaagtcgtcc agcgcgatgt gacagcccag cttgcgcagc cgtcgcatca gcggttccgc
      121 ccggctaagg ttgccgatgg ccgcgctctc ggtcagttcg aagcacagcg aacccggatt
      181 cagagcgctg tcttccacca gtttgaccac gtattcggtg aactccgcgt cctgcagcga
      241 ttgaccggag aaattgatgt tgaacacgat ctggctggcg ctcagcactt ccgcatgcgg
      301 tttcagtatc tcgatcgaac gcgcgattac ccaacggtcg agcgtgggca tcaactggta
      361 gcggtgcgcg gccgacatga agtggtcggg gccgacgatc tcgccgttct cacccaccat
      421 gcgcaacagc acttcgaagt gcggcggccc gcgatgcccc cccaggggca cgatcagctg
      481 cgcgttgagt tcgagccggt tgtcagcgat cgcgccgcgc aagtcggcaa tcaggttgat
      541 gtccgtgaaa cggcgcacga tgctctcgtc ggtttcctga tagacctcga cgcggttgcg
      601 cccgcgatcg ttggccgcct tgcaggcggt ctcggcggct gcgaaagcgt gcaccagttc
      661 gttcgacttg ggatcgaccg ctgacacccc gatgctaatc gatacctgca tcttgccgtc
      721 gccgaaggcc gcgccgactt cttcggcgcc ggaacgcaac gcctcggcga acttggcggc
      781 atcctccagc ttcgcaggca acaggatcgc gaagcgatcg ccggagattc tcgccgcaat
      841 ggcgccaccg ggcggcaggc gcgtacggat cagctcaccg agctgagcga tcacccggtc
      901 gcccattggc atgccgtagt tgtcgttgat gacgtgcagg cggttgatat cgatgaacag
      961 cgcactccac gcgcgcttga agccctgacc tttggcggtc cggtcctcgt cgagcgtggc
     1021 tcgaacgcgc tgttcgaacg ccggacgcgt cagcaggcca gtcagcgcgt cgtagctgtg
     1081 cgtcacaatc gaggcgacgc ggcgcgccag caactcggtg agacgcccgt gatgggctgt
     1141 gaattcgggt gcgctctcgg ctcgaaacag tgccaggact cccatcgccc ggccgtcggg
     1201 acgcgcgatc gggcaggcca gcacccgata agccgcgcag ttctgaccct gctgcaatac
     1261 catgcgattg acgatcgtgg cctgacgccg cacatgcgcc atcgacagca gatgccgatg
     1321 tgctttggcg agcaatgagg tctccagcgg cgcatcggcg gagacctgaa ccatcgcgat
     1381 gcccttctcg ggcacgatca gcgctgacag gccgactcgc agatgttcgg tcgcactctg
     1441 aacgatcgcc ttcaggtcgt tgctgttttc cgctttgctg tcgccgccac cggataccga
     1501 cagcagcatc tccagatcat tgtcctgctc gagcatcgag ctctgcagat tcagaatctc
     1561 atcgcgcgcg agcagctcgc gccgcagaca ttcgatcgca ggcttcacca acgcctgcac
     1621 gaagctgaat gggcgttgct cgtgctcgcc attgcccggt ctgaagttca aactcacgac
     1681 cgcaaacggg gttgccggcc gtgagccgtc gtcgcgtcgc aaccagaaca ggtacaccgc
     1741 ctcgccgcgg gcaaaccgtt gctcgcccga ggcgaccggc tcggcctcgg cggtcttgat
     1801 cgacagcggc acaatgccgc tgagctccgg cccgacccct tcgctgctcc agtagagttc
     1861 accgcgctca ttgaacacgc tcaacgtgtc catgcgtggc agcagcgcgc gcagcaactg
     1921 cgcgtacggc tccagcgagg acgaagcggt cgcgggacgc cctagggcgg gtgtattcat
     1981 tgcgttctat ggggtttcaa gagcgtcagc atcgaatatg aagcgaccat aatccttgac
     2041 ggaggtcgct attcaggcga gcaaagcgag accccgtgct cactgcccga gtcgttcagg
     2101 gagggggact ggaggtccga aaccacggtg taggatcaac cggtttgcct gcgcggcgga
     2161 tctcaaaata gagctccggc gaggcccttc cgccggtatc tccggccgcg gcgaccgcct
     2221 ctccgccggc caccgggtcg cctgccgcct tgagcagccg atcattatgt ccgtacaggc
     2281 tcatgtagcc ttccccgtgg tcaatgatca ccagcagtcc cagccccggc
//
LOCUS       DATMRM010000151         6704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1649298, whole genome shotgun sequence.
ACCESSION   DATMRM010000151 DATMRM010000000
VERSION     DATMRM010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1649298"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VNZ06_04435"
     CDS             <1..548
                     /locus_tag="VNZ06_04435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HWW20030.1"
                     /translation="HEADALDFDWSSLARKLGAPLRLIGNLPYNISTPLLFRLLAAAP
                     VIRDMHFMLQKEVVDRIVADPGSRNYGRLTVMLAPRVSAARLLDVGAGAFHPVPRVAS
                     SVVRLTVLREPPDWAQSEMYGKVVLAAFSQRRKTLRNALRAYLTAPQMSAAGIDPGGR
                     AETLSPAQFGALAQAAAAVFH"
     gene            553..984
                     /locus_tag="VNZ06_04440"
     CDS             553..984
                     /locus_tag="VNZ06_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWW20031.1"
                     /translation="MPDYHRILAAIDLTEDSRSVARRAVEIARIANGSVHLLHVLEFV
                     PVEPMSDTLVPVVQIDDQMLARARAQIQALAAELGLPADSATVEAGSAKSEILRHARQ
                     RNVDLIVLGCRERHGLSILVNRTEDTVLHGAPCDVLAVRVR"
     gene            987..1358
                     /gene="apaG"
                     /locus_tag="VNZ06_04445"
     CDS             987..1358
                     /gene="apaG"
                     /locus_tag="VNZ06_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019650335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HWW20032.1"
                     /translation="MRHKITIEVETNYLDEQSEPREQRYVFAYTITIRNEGDVPAKLL
                     TRHWIITDSNGRVQEVRGDGVVGEQPYLKPGQGFRYSSGAVLETPVGTMQGSYQMLAD
                     DGAKFEAPIPAFRLAIPGMLH"
     gene            1360..2190
                     /locus_tag="VNZ06_04450"
     CDS             1360..2190
                     /locus_tag="VNZ06_04450"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HWW20033.1"
                     /translation="MARWAIGDVQGCCDELEELLHRIRFSSERDQIWFVGDLVNRGPR
                     SLHTLRLVRSLGENALCVLGNHDLHLLAVALVGAKLRRNDTLNDILAAPDRDSLIEWL
                     LYRPLAHYEPQRGDLLVHAGVVPQWSAGLTLELAREVEQALRHDARGLLSTMYGDEPD
                     HWQPALSGVDRRRFTVNVLTRMRFMTVEGRIDLKQKGKPQSAHKPLIPWFKAAHRQTA
                     QLRIIFGHWSALGLHQEPNILGLDSGCVWGGSLSAANLDDPGSPIVSVPSRQPRSIEQ
                     "
     gene            complement(2150..2599)
                     /locus_tag="VNZ06_04455"
     CDS             complement(2150..2599)
                     /locus_tag="VNZ06_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20034.1"
                     /translation="MPGRSGLRGALLATLCLALSACAMHWHWPWHHRQKPPPPAVHQV
                     TVESDSAASILQFWDRNTLQFDLTALSGEGSATISAVKSVGWPVRLEFLVRPGSFGRL
                     EVVGSQRVVFQVPPQGKNLVLKLAPGTYLPDTSSITVRWNAADDSAH"
     gene            2683..3225
                     /locus_tag="VNZ06_04460"
     CDS             2683..3225
                     /locus_tag="VNZ06_04460"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF009763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HWW20035.1"
                     /translation="MRALRPIHFKQWIDANRHLLRPPVGNKVIYTDSEFIIQVVGGPN
                     SRKDFHVDPAEEFFYQLEGRMVLRILENGNIVDVPIPAGDILLLPPQTPHSPQRHPDS
                     VGLVIERRRRPGELDGLQWYCENCSHLLYEEFFPLTNIEAQFQPVFDRFFGSLSLRTC
                     KRCHAVMEPPTSVPETHGTQ"
     gene            3212..4054
                     /locus_tag="VNZ06_04465"
     CDS             3212..4054
                     /locus_tag="VNZ06_04465"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWW20036.1"
                     /translation="MARNERPQWARLDIDGIGLLADLAAGTSLARMLDFKGAQLRCFS
                     APAASSKALQTADFTGRVERGGSCNCNVLTLTPHANGTHTECVGHLTAQPLDVYKVIP
                     QRLLIATLLTVEPELAGDSEDSSRPPPRATDLLITRAALERAWPAAALARLSGRSAVI
                     RTLPNARDKFVEDGGASAPYLSSEAAAWLVARGIEHLVLDIPSADRTSDSFEMTAHRI
                     FFGLADGNTTLGDAQRRDCTITELAFVPESVTDGWYFLSLQIPAITGDAIPSRPVLYP
                     LRTA"
     gene            4051..5343
                     /gene="kynU"
                     /locus_tag="VNZ06_04470"
     CDS             4051..5343
                     /gene="kynU"
                     /locus_tag="VNZ06_04470"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HWW20037.1"
                     /translation="MSGESFDQQLSWARAQDAADPLAALRKEFVLPTDAYGRPLLYLC
                     GHSLGLAPRNARAQVDVELERWERLGVLGHHSGQPRWIDYAESLQPLLAQLAGALPQE
                     VTAMNSLSVNLHLLLASFYRPRAERRAILIEAGAFPSDRHVVASQIAWHGGDPARDLI
                     ELAPRSDEDLLRAEDIEASIASQAAHLALVLWPGVQYRTGQQFDLAAIARAAHAAGAN
                     AGFDMAHAIGNVPLSLHADEADFAVWCSYKYLNAGPGAVGGAFVHERHLKRADLPRLT
                     GWWGHHSGTRFEMSHQFEPAEGTAAWALSNPPILSSAPLRASLPLFASAGMPALRAKS
                     IGLTGYFETLLKQLAGEHLRLVTPADPAQRGSQLSVRLIHGAERGRRIFQALTARGIV
                     ADWREPDIIRLAPAPMYNNYEEVLRSAWHLGEILSARP"
     gene            5347..>6704
                     /locus_tag="VNZ06_04475"
     CDS             5347..>6704
                     /locus_tag="VNZ06_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWW20038.1"
                     /translation="MADFTIVGAGPVGALMGLLLAQRGHRVHLRERRPDPRLTPTERG
                     RSINLALSARGLVALGRAGVLERVRASMITMPGRMLHDERQQLEFLPYGQNQHEVNYS
                     VSRAQLNRVIVEAAGEHPAIDLRFHQRCVDLDPHSGMVQLRDEQTGQLHSESTQILLG
                     ADGAGSSVRGALVKHGLTRINEMPLPHDYKELAIAPVADSRHGGYAFEPRALHIWPRG
                     GFMLIALPNMDRSFTATLFLPRHGDCSFERLQDSRVLGEFFRYQFEDAAVVIPDLHQQ
                     FQQHPQSHLGTIDCQGWHAGGRVLLIGDAAHAVVPFHGQGLNCGFEDCVVLDRLLSQS
                     PDVATALASFERERQPNTSALAEMALENYVEMRDDVRSPEFAPRKALATSLERAFPGR
                     FIPRYSMVTFHPEISYAEARRRGAQQQVVLERLVEEFPAIDSDELPPEAVTAARQYLQ
                     EAG"
BASE COUNT         1192 a         2183 c         2129 g         1200 t
ORIGIN      
        1 tgcatgaagc cgacgccctc gacttcgact ggagttccct ggcgcgcaaa ctgggtgcgc
       61 cgctgcgcct gatcggcaac ctgccctaca acatttccac tcccctactg ttccggctgc
      121 tcgcggcggc accggtcatt cgcgacatgc acttcatgct gcaaaaggaa gtcgtggatc
      181 gcattgtggc cgatccgggc agccgcaact atggccgcct gacggtcatg ctggctccgc
      241 gtgtgtcggc ggcgcgccta ctcgatgtcg gggccggcgc tttccacccg gtcccaaggg
      301 ttgcctccag cgtcgtgcgg ttgacggtgc tgcgcgagcc gcccgattgg gcgcagtccg
      361 agatgtatgg gaaagtggtg ctggccgctt tcagccagcg gcgcaagaca ctgcgcaacg
      421 cactgcgcgc gtacctgacg gcgccgcaga tgagcgccgc gggaatcgat ccgggcgggc
      481 gtgctgagac cctgtcaccg gcgcaattcg gcgcgctggc gcaggcagcc gccgctgtgt
      541 ttcactaatg gcatgcccga ctatcaccga atcctggcgg ctatcgatct gaccgaggac
      601 agccgttcgg tagcgcgccg ggcggtagag attgcccgca tcgccaatgg cagcgtgcac
      661 ctgttgcacg tgctcgagtt cgttccggtc gaaccgatga gcgataccct ggtacctgtg
      721 gtacagatcg atgatcagat gctcgcgcgc gcgcgcgcgc agatccaggc gctggcagcg
      781 gaattggggc tgccagccga ttcggccacg gtggaagcgg gcagcgcgaa aagcgagatc
      841 ctgcgccacg cccgtcaacg caatgtcgat ctgatcgttc tgggctgccg tgagcgccac
      901 ggtctgtcga ttctcgtcaa tcgcacggag gacacggtat tgcacggcgc gccgtgcgat
      961 gtgctggccg tccgggtacg ctaggcgtgc gccacaagat taccatcgag gtggagacca
     1021 actacctgga tgaacaatcc gagccgcgcg aacagcgcta cgtcttcgcc tacaccatca
     1081 cgatacgcaa tgaaggcgat gtaccggcca agctgctgac acgccactgg atcatcaccg
     1141 attccaacgg gcgcgtgcag gaagtgcgcg gcgacggcgt ggtcggcgaa caaccctacc
     1201 tcaagccggg acagggattt cgatactcca gtggcgcggt actggaaact ccggtcggta
     1261 ccatgcaggg cagctatcag atgctcgctg atgacggtgc caagttcgaa gcaccgattc
     1321 ccgcgttccg cctggccatc cccggcatgt tgcattgaga tggctcgctg ggcgatcggc
     1381 gacgtccagg gctgctgcga cgaactcgaa gaactgctgc atcgtatccg cttctcgagc
     1441 gaacgcgatc agatatggtt cgtcggcgac ctcgtcaatc gcggaccgcg ctcgctgcac
     1501 accctgcggc tggtgcgctc tcttggcgag aacgcgctgt gcgtactggg caatcacgat
     1561 ctgcacctgc tggcggttgc gctggtcggc gccaagctgc gcaggaacga taccctgaat
     1621 gacatactcg ccgcgcctga tcgcgactcg ctgatcgagt ggctgctgta ccggccgctg
     1681 gcgcattacg agccgcagcg cggcgatctg ctggtacatg ccggcgtggt gccgcagtgg
     1741 agcgcggggc tgacactgga gctggcgcgc gaggtcgagc aggcgctgcg gcatgacgcc
     1801 cgcggactgc tctcaacgat gtacggcgat gagcccgatc actggcagcc tgcgctatcg
     1861 ggcgtggatc gccgacgatt taccgtcaat gtgctcacgc ggatgcgatt catgacagtc
     1921 gaggggcgga tcgatctcaa acagaagggc aaaccgcagt ccgcacacaa gcccctgatc
     1981 ccgtggttca aggcggcgca ccggcagacc gctcaactgc gcatcatctt cggccattgg
     2041 tcggcgttgg gtctgcacca ggagccgaac atcctgggac tggacagcgg ctgcgtgtgg
     2101 ggaggttcac tcagcgccgc caatctggac gatccgggct ccccaatcgt cagtgtgccg
     2161 agtcgtcagc cgcgttccat cgaacagtga tgcttgacgt gtccggcaga taagtacccg
     2221 gcgcgagctt cagcaccaga ttcttgccct gtggcggtac ctggaacacc acgcgctgtg
     2281 agcccacgac ttccaggcgt ccgaagctgc ccggacgcac cagaaactcc agccggaccg
     2341 gccagccgac cgacttgacg gcgctgatcg tcgcgctccc ctcaccgctc agcgccgtca
     2401 gatcgaactg cagggtattg cggtcccaga actgaaggat gctggcagca ctgtcggatt
     2461 cgaccgtcac ctggtgtacc gcaggcggtg gcggcttctg cctgtggtgc cagggccaat
     2521 gccagtgcat cgcgcaggct gacagcgcca gacacagcgt cgccagcaac gcacctcgaa
     2581 gacccgatct acccggcata tggttcaaag tcgcctccac taggcaatac gcttaccata
     2641 cgcgaactat agccgtcgtg agatttgtcg gaaaacctcg acatgcgtgc cttgagacct
     2701 atccatttca agcagtggat cgacgccaac cgacacctgc tgcggccgcc agtcggcaac
     2761 aaggtcattt ataccgatag cgagttcatc attcaggtgg tcggcggacc gaatagccgc
     2821 aaggacttcc acgttgatcc ggccgaagaa ttcttctacc agcttgaagg caggatggtg
     2881 ctgcgcattc tggagaacgg caacatcgtc gacgtgccga ttccggccgg ggacatcctg
     2941 ctgctgccgc cacagacgcc gcattctccg cagcgccacc cggactcggt ggggctggtc
     3001 atcgagcggc gacgccggcc gggcgagttg gatggcctgc agtggtattg cgagaattgc
     3061 tcccatctac tgtatgagga attctttccg ctcaccaaca tcgaggcgca gttccagccg
     3121 gttttcgatc gtttctttgg cagcttgtca ctgcgcacct gcaagcgttg tcacgccgtc
     3181 atggagccac cgacttcggt tcccgagaca catggcacgc aatgagcgcc ctcagtgggc
     3241 acgtctggac atcgatggaa tcggactgct ggccgatctg gctgccggca cgagcctcgc
     3301 tcgtatgctc gatttcaaag gcgctcagct gcgttgcttc agtgctcccg cggccagcag
     3361 taaagcgctg cagaccgccg atttcaccgg tcgcgtcgag cgcggcggca gttgcaactg
     3421 caatgtcctg actctcacgc ctcatgcgaa cggcactcac accgaatgcg tggggcacct
     3481 gacggcgcaa ccgctggatg tctacaaggt cattccgcag cgcctgttga tcgcgacgct
     3541 cctgacggtc gagcccgaac tggcgggcga cagtgaagac agcagcagac cgccgccgcg
     3601 ggcgacggat ctgctgatca cgcgggcagc gcttgaacgc gcctggccag ccgcagccct
     3661 cgcgcggctt tccggacgca gcgccgtgat ccgtacgcta cccaacgcgc gcgacaagtt
     3721 cgtcgaggac ggcggtgcct cagcacccta cctctcgagt gaagccgccg cgtggctggt
     3781 cgcgcgcggc atcgaacacc tggtgctcga cataccctcg gccgatcgca ccagcgacag
     3841 tttcgagatg accgcgcacc gcattttctt tgggctggcc gacggcaata ccacgctcgg
     3901 cgacgcgcag cggcgtgact gcacgatcac cgaactggcg ttcgtacctg aatccgtgac
     3961 cgacggctgg tacttcctgt cactacagat tccggcaatc acaggcgatg ccatcccttc
     4021 acgtcccgtg ctctatccgc tgcgcacggc atgagcggcg agtccttcga tcagcagctc
     4081 agctgggcac gcgcgcagga cgcggccgac ccgctggcgg cgttgcgcaa ggaattcgtt
     4141 ctgccgaccg atgcctatgg cagaccgctg ttatacctgt gcggccattc actgggactg
     4201 gcgccgcgca acgcacgcgc ccaggtggac gtcgagctgg agcgctggga gcgtctgggt
     4261 gtgctggggc accacagtgg acagccgcgc tggatcgact acgccgagag cctgcagcca
     4321 ttgctggcgc agcttgccgg cgcgctaccg caggaagtca cagcgatgaa ctcgctgagc
     4381 gtgaatctgc atctgttgct ggcgagtttc taccgaccgc gcgccgaacg ccgcgcgatc
     4441 ctgattgaag ccggcgcctt cccctccgac cgacatgtgg tggcatcgca gatcgcctgg
     4501 cacggcgggg atcccgcgcg cgatctgatt gagctcgcgc ccaggagcga tgaggacctg
     4561 ctgcgggccg aggatatcga ggcttcgatc gcttcacagg ccgcacacct ggccctggtt
     4621 ctgtggcccg gagtgcaata ccgcaccgga cagcagttcg atctggccgc gatcgcccgc
     4681 gccgctcatg ctgctggcgc gaacgccggc ttcgacatgg cgcacgccat tggcaacgtg
     4741 ccgttgtcgc tgcatgcgga tgaggccgat ttcgctgtct ggtgcagcta caaatacctc
     4801 aatgccggcc ccggcgcggt cggcggcgcg ttcgtgcacg agcggcatct gaaacgcgcc
     4861 gatctgccac ggctgacggg ctggtgggga catcattccg gcacgcgctt cgagatgtcc
     4921 caccagttcg aaccggccga ggggaccgcg gcgtgggcac tcagcaatcc accgatcctc
     4981 tcgagcgcgc cgctgcgcgc atcgctgcca ctgttcgcaa gcgcgggcat gccggccctg
     5041 cgtgccaaat ccatcggtct gaccggttat ttcgagacgc tgctgaagca gctcgcaggc
     5101 gagcacctgc gcctggtgac accagccgat ccggcgcagc gcggcagtca gctctcggta
     5161 cggctgattc acggcgccga gcgcggccgc cgtatattcc aggcactgac ggcccgcggt
     5221 attgtcgctg attggcggga gccggacatc atccgcctgg ctccggcgcc gatgtacaac
     5281 aactacgaag aagtcctgcg cagcgcgtgg cacctgggcg agatcctgtc cgcgcggcct
     5341 tgaacgatgg ccgacttcac gatcgttggc gccggaccgg tcggagcgtt gatgggtctg
     5401 ctgctggcgc agcgtggaca ccgcgtgcac ctgcgagagc gccgacccga tccacgcctc
     5461 acgcctaccg agcgtggccg ctcgatcaac ctcgcgctgt cggcacgcgg gctggtggca
     5521 ctggggcggg caggagtgct cgaacgtgtc agggcttcga tgatcacgat gccgggtcgc
     5581 atgctgcatg atgaacggca gcagctcgaa ttcctgccct atggtcagaa ccagcacgag
     5641 gtcaactatt cggtcagccg cgcgcagctg aaccgcgtga tcgtcgaggc tgccggtgag
     5701 catccagcga tcgatctgcg cttccaccag cgctgtgtcg atctcgatcc gcactccggc
     5761 atggtgcaac tgcgcgacga gcagactggc cagctgcaca gtgagtcaac ccaaatcctg
     5821 ctgggcgccg atggcgccgg gtccagtgta cgcggcgcac tggtcaaaca cggcctgacc
     5881 cgcatcaacg agatgccgct gccacatgac tacaaggaac tcgcgatcgc cccggtcgct
     5941 gatagccgcc acggcggcta tgcattcgag ccccgggcat tgcacatctg gccgcggggc
     6001 ggcttcatgc tgatcgcgct gcccaacatg gatcgcagct tcacggcgac gctgttcctg
     6061 ccgcggcatg gcgactgcag cttcgagcga ctgcaggaca gtcgggtgct gggggaattc
     6121 ttccggtatc agttcgagga tgccgccgtt gtcatcccgg atctgcacca gcagtttcag
     6181 cagcatccgc agagccacct cggcacgatc gattgtcagg gatggcacgc aggcggtcgc
     6241 gtgctgttga tcggcgatgc cgctcacgcc gtcgtgccgt ttcacggtca gggtctgaac
     6301 tgcggattcg aagactgtgt ggtactcgac cgactgctat cacagagccc ggacgtcgcc
     6361 accgcactgg cctcgttcga gcgcgagcgg caacccaaca ccagcgcact ggcggaaatg
     6421 gcgcttgaga attacgtgga gatgcgcgat gacgtgcgct cgccagagtt cgcacctcgc
     6481 aaggccctgg ccacttccct ggaacgggcg tttccgggac ggttcattcc acgctactcc
     6541 atggtgacat ttcatccgga gatctcctat gccgaggcac gcaggcgcgg cgcacagcag
     6601 caggttgtgc tcgagcgtct ggttgaggaa tttccagcga tcgattcgga tgagctgccg
     6661 cccgaggcag tgaccgctgc gcgccagtac ctccaggagg cggg
//
LOCUS       DATMRM010000152         6005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_702632, whole genome shotgun sequence.
ACCESSION   DATMRM010000152 DATMRM010000000
VERSION     DATMRM010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702632"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VNZ06_04480"
     CDS             <1..1357
                     /locus_tag="VNZ06_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HWW20039.1"
                     /translation="TLDLQYFIFRGDQTGRLLTAALLRAADRGVRVRVLVDDGDVAAG
                     DEQILALDGHAGAEVRYFNPFAYRGRSRFRRSLEFLFNGRRLDYRMHNKLLVVDNSVA
                     LVGGRNIGNQYFQVDPDSQFADDDVFAAGPIVRQLSATFDEYWNSELALPARALSPVK
                     PTSTASTHSRAGSHKSSQRLAQTSKSNGVDYLGRSASGEPYAGILSGRLALVWAPAEV
                     VCDNPEKRKIEHGARAGKLMAPSVVAAANGAQTELLVVTPYFVPSDDELTILKDLRQR
                     NAHVGILTNSLETAPGLTAYAGYTHYRVPLLKAGVNLYEARARLGNARGSGQTARLSR
                     YGHYALHGKLFVIDRQRVFIGSMNYDQRSRNINTEVGLIIDSGELARQTAARFTAMVQ
                     MDNAYTLALRPGGANIKSRLVWDTLENGKPIEYQREPARSSWQRMRARWLSLLPLDSE
                     L"
     gene            complement(1433..1837)
                     /locus_tag="VNZ06_04485"
     CDS             complement(1433..1837)
                     /locus_tag="VNZ06_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20040.1"
                     /translation="MHYVSVIIEILLVGYIVRDAARAQSLYRQLKQATANGDAGARSR
                     MYREILAFEWISAVLALGALGFDWSKLTPRSLVLADSLWMPQLANHPEFFRGVGREDH
                     RRAGNGTSWCVLLRALYRDRIVAATDHPARAH"
     gene            complement(1854..2408)
                     /locus_tag="VNZ06_04490"
     CDS             complement(1854..2408)
                     /locus_tag="VNZ06_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20041.1"
                     /translation="MIDAELDSWKREWRERTDPFPALKRKISRQNLKTGAAIVVLLLL
                     MALSLAGALHYATAFMYGAVVGVWTLVPILGGYVWWVARGAWKATAQTTVAYAELAHR
                     RAVARARTLRFGLIYLLVAWVLGAGVLAWDWRHFSTSLALVLLGLLIELLVFRYYLQP
                     RRQRQIEEAAKLVDYIRELTRSET"
     gene            complement(2405..2929)
                     /locus_tag="VNZ06_04495"
     CDS             complement(2405..2929)
                     /locus_tag="VNZ06_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWW20042.1"
                     /translation="MNKATAESAQQRFDRIMREYGPALSRLTAGYEKVASVREELMQE
                     IALAIWQALPQFRGECSERTFVYRIGHNRGLTHTYRRPAAHQRLDELPHLELVDPQPR
                     PDQQVATIDQHDRLRSAIQTLPLTYRQVVMLWLEDLAQADIAAVLGITEGNVAVRLNR
                     ARAALKQALQERTR"
     gene            complement(3203..4183)
                     /locus_tag="VNZ06_04500"
     CDS             complement(3203..4183)
                     /locus_tag="VNZ06_04500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWW20043.1"
                     /translation="MGKQQAGFHPASYALWYEHAAGVNPSLSRALEERISDSTPLTDI
                     DVRNLYTRHIVSRDRETIERVEERLAELLHETSQVISHTGVHFMQFGATLEGHATQLQ
                     QPTALESLQHIVSELLAETQQMRVTNLTLTRQLDTSAQEVLTLTERLARAQVEALNDP
                     LTRLLNRRGFEEVVSKLYDGTAPLAGASLLLIDVDEFKAINDTYGHLVGDQVLRAVAQ
                     IVRARIKGADIASRLGGDEFAVLLPDTSIAGATALAEQIRTTLLQRRLRLVGHDQYVE
                     NVTLSIGIANVAKEASLESLLERADLALYAAKRSGRNRVSTTADPTKPTS"
     gene            complement(4379..5680)
                     /locus_tag="VNZ06_04505"
     CDS             complement(4379..5680)
                     /locus_tag="VNZ06_04505"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWW20044.1"
                     /translation="MNKPHLSWLGCAVLFISFAASADTVIVTADRMLDVVAGRVVDHP
                     QITITDGRIVSVGSKDAATAPTADVRRVNLSGMTLLPGLIDMHVHLTIDPHFSGYRGL
                     EFTDNFWTVVGVANAKRTLDAGFTTVRNVGSANFDDVALKQGIEQGYIQGPRIVPATY
                     AIGATGGHCDDTEFPPSITTPDTNIANGPENIRAMVRKLRKYGAEVIKFCGTGGVLSK
                     TDSVGGQQYDLTEMKALVDEAHMLGLKVAVHAHGTAGIKDALRAGVDTIEHASLADEE
                     AFALARQHGAYFDMDIYDDDYILAEGEKNGVFKESLEKERSIGRKQRETFRAALKAGV
                     KMIFGTDAGVYPHGDNAKQFVTMVTWGMTPLQAIQAATVTAAEALGRSADVGAIAVGR
                     YGDLIAVRGDPLTDVSVLQSVGFVMKGGQVVKQPVPPTPAN"
     gene            complement(5714..>6005)
                     /locus_tag="VNZ06_04510"
     CDS             complement(5714..>6005)
                     /locus_tag="VNZ06_04510"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HWW20045.1"
                     /translation="RLVADTRAAVVLREATYPDVHYFPRQDVDMSLLMRTAHSTYCPY
                     KGECCYYSIPLGKDRSINAVWTYEDPYPAVATIKHYLAFYPDRVDAIEKMDN"
BASE COUNT         1168 a         1859 c         1791 g         1187 t
ORIGIN      
        1 aaccctggac cttcagtatt tcatctttcg cggggatcag accggccgac tgctcacggc
       61 tgcgttgctg cgagcagccg accgcggtgt ccgcgtgcgg gtcctggtgg acgacggcga
      121 cgtcgcggct ggggatgagc agatcctcgc gctcgacgga catgctggtg ccgaagtcag
      181 gtacttcaat ccgtttgcct atcgcgggcg ctcaagattt cgccggagcc tggagttcct
      241 gttcaatggg cgccgcctcg actatcggat gcacaacaag ctgctggtgg tcgacaattc
      301 tgtggcgctc gtgggcggac gcaacatcgg caatcaatac ttccaggtgg atccggactc
      361 ccagtttgct gacgatgacg tcttcgccgc gggtccgatt gtcaggcagc tctccgcaac
      421 cttcgatgag tactggaata gcgagcttgc gctaccagca cgcgcattga gccctgtcaa
      481 acccacatca acggcgagca cgcattcgcg cgcggggtcg cataagtcat cgcaacggtt
      541 agcgcagacg tccaagtcca atggcgttga ctacctaggc cgcagtgcca gcggggagcc
      601 ctacgccgga atcctctccg gccgcttggc gctggtctgg gcgcctgcgg aagtggtttg
      661 tgacaacccc gaaaaaagaa aaatcgaaca tggcgcgcgc gctggcaagc tgatggctcc
      721 ctcggtagtg gccgctgcca atggcgcaca aaccgagctg ctagtggtca caccgtactt
      781 cgtgccatcg gacgacgaat tgacgatcct gaaggatctg cgccagcgca acgcgcacgt
      841 cgggattctc accaattcgc tcgagacagc ccctgggctg accgcctatg cgggttacac
      901 gcactatcgg gtgccgctac tcaaggccgg cgtcaatctg tacgaggcgc gcgcgcggct
      961 gggcaatgct cgcggcagcg gtcagacagc caggctgtcc agatatggcc actacgcgtt
     1021 gcacggcaag ctgttcgtca ttgaccgcca gagagtcttc atcggctcga tgaactacga
     1081 tcaacgatcg cgaaatatca ataccgaggt cggactgatc atcgatagcg gcgagcttgc
     1141 ccggcagacg gcggcgcgat tcacggccat ggtccagatg gacaatgcct acacgttggc
     1201 actgcgcccg ggcggagcaa atatcaaatc acgcctggtg tgggatacgc tggaaaacgg
     1261 caaaccgatc gaataccaac gcgagccggc acgcagcagc tggcaaagaa tgcgagctcg
     1321 atggctgtca ctgctgccgc tggatagtga gctttgagtg acccgcggcc ctcaagccgg
     1381 cactgcgtcg agtttgcgcg ggcgcggtgc gggcatcagc gcccaccgta cgtcaatgag
     1441 cgcgtgcagg atgatcggta gcagcaacga tccggtcgcg atatagagcg cgcagaagaa
     1501 cgcaccaaga agtcccgtta ccagcacgcc ggtgatcttc tcttccgacg ccgcgaaaga
     1561 actcgggatg gtttgcgagc tgcggcatcc acaggctgtc cgctagaacc agacttcgag
     1621 gcgtcagctt gctccaatcg aatccgagcg caccgagcgc cagcaccgcg ctgatccact
     1681 cgaacgcgag aatctcacga tacatgcggc tgcgcgcacc ggcatcgccg ttggcagtcg
     1741 cctgtttgag ctgtcggtag agcgactgtg cccgggctgc atcgcggacg atatatccga
     1801 ccagcaggat ttcgatgatg acggatacgt aatgcatgag cgtctacctt tcgttaggtt
     1861 tccgagcggg tcagttcgcg gatgtagtcc accagtttcg cggcttcttc gatctgccgc
     1921 tgccggcgcg gctgcaggta gtatcgaaat accagcagtt cgatcagcag cccgagcaga
     1981 accagcgcca atgaagttga gaagtggcgc caatcccagg ccagcacgcc tgcgcccagt
     2041 acccaggcga cgagcaggta gatgagccca aagcgtagcg tgcgcgcgcg cgccacggct
     2101 cggcgatgcg ccagctcggc gtaggccaca gtggtctgtg cggtcgcttt ccacgcccct
     2161 cgcgccaccc accagacgta accgcctaag attgggacca gcgtccacac gcctactacc
     2221 gccccataca tgaatgccgt tgcgtagtgc agcgctcctg cgagcgacaa tgccatgagc
     2281 agcagcaaca cgacgatggc cgcgccggtc ttgagattct gtcgcgagat cttgcgcttg
     2341 agcgcaggaa acggatcggt ccgttcgcgc cattcgcgct tccagctgtc cagttcggcg
     2401 tcgatcatcg ggtgcgctcc tgcagcgcct gcttcagcgc tgctctggcc cgattcaggc
     2461 gtaccgcgac attgccctcc gtgattccga gcacagcggc gatgtcggcc tgggcgaggt
     2521 cttccagcca cagcatcacg acctgccggt aggtgagtgg caacgtctga atggccgatc
     2581 gcagccgatc gtgctgatca atcgtcgcga cctgctggtc tgggcgaggc tgcggatcga
     2641 ccagttccaa atgcggcagc tcatcgaggc gctggtgtgc cgccggtcgt cgataggtgt
     2701 gagtcaaacc gcggttatga ccgatgcggt agacgaaggt gcgttcggag cattcgccgc
     2761 gaaactgcgg cagtgcctgc catatcgcca gggcaatctc ctgcatcagt tcctcacgca
     2821 cgctcgctac cttttcgtac cctgcggtaa ggcgcgacaa ggcgggcccg tactcgcgca
     2881 tgatgcgatc gaagcgctgc tgcgcactct cagcggttgc cttgttcatg gctcgagcgc
     2941 gctcgtcgta tcccccgatg ccaggacatg gcgtggtgtc gaccagcaag ccaagatctc
     3001 ctcgtcatat tcttgatcta tgagaggtag tctcggggtg tgcgaaaacc ttacggggct
     3061 cgttggaagg cgccttggca acgtgggccg aagcttctag ccaccggggc tcggcagttt
     3121 agcgcctcgc gtgtacccca ttgattttaa acggccccag ccgacccgta gacgctggag
     3181 ccgtcgctgc ctggacgcgg agttagctgg tcggcttggt cggatcggcg gtggtggaga
     3241 ctcgattccg gccagatcgt ttagcggcat acagggccag atccgctcgc tccagcagcg
     3301 actccaacga agcttccttg gccacgttcg cgataccgat cgacaacgtg acattctcaa
     3361 cgtattgatc gtgaccgacc aatcgcaggc gtcgctgcaa cagagtggtc cgaatctgct
     3421 cggccagtgc cgtggccccg gcgatgctgg tgtcgggcag tagcactgca aactcgtcac
     3481 cccccagccg tgacgcgatg tcggcacctt tgatgcgagc gcgcacgatc tgcgccacag
     3541 cgcgcagcac ctggtctccc accagatggc cataggtatc gttgatggcc ttgaactcat
     3601 cgacatcaat caacagcagg gaggcgccgg ccagcggcgc ggttccatcg tagagctttg
     3661 agacgacttc ttcgaagccg cggcgattaa gcagtcgcgt cagcggatca ttcagcgctt
     3721 ccacttgcgc gcgcgccaat cgctccgtca gggtgagcac ctcttgagca ctggtatcca
     3781 gttgccgggt cagcgtcaga ttggtgactc gcatctgttg cgtctccgcc agaagctcgc
     3841 tcacgatgtg ctgtagcgat tccaaagcgg tgggttgctg caactgcgtt gcgtgccctt
     3901 ccagggttgc gccgaattgc atgaaatgaa ccccggtgtg ggaaatgacc tgggaggtct
     3961 cgtggagcaa ttcggccaga cgttcctcca cgcgttcgat ggtttcgcgg tctcgcgaca
     4021 cgatgtgccg ggtatagaga ttcctgacat cgatgtcggt cagcggcgta ctgtcggaaa
     4081 tacgctcctc gagcgcccga ctgagcgagg gattcacgcc agccgcatgt tcgtaccaga
     4141 gcgcatagct ggccggatga aatccggctt gctgcttgcc catgaggccg acgacaattc
     4201 gtaagagttc cgcgctctgt tccctactct gttgatagtc catactttcg gctctcaact
     4261 cctttccacg gtgaaatacg cggcgttcct aagggaagtg cttgacgtcc cctaccgttg
     4321 ctgcaatgcg aatatagccc atcagaaatc aaactgtaag ttgccgacgc gaactccatc
     4381 agttcgccgg ggtgggcgga accggttgtt tgacgacctg cccgcccttc atcacgaacc
     4441 ccactgactg cagcacactg acgtccgtca gcggatcacc cctgaccgcg atcaggtcgc
     4501 cgtagcgacc caccgcaatg gcgccgacat cggcgctgcg accgagggcc tcggcagccg
     4561 tgacggtcgc ggcctggatg gcctgcagcg gcgtcatgcc ccaggtcacc atcgtgacaa
     4621 actgcttcgc gttatcccca tgagggtaga cgccggcgtc cgtgccaaaa atcattttga
     4681 caccggcctt cagtgccgcc ctgaaagtct cacgttgctt gcgcccgatg ctgcgttctt
     4741 tttccaacga ttccttgaag acgccgttct tctcgccttc cgccaggatg tagtcatcat
     4801 cgtagatgtc catgtcgaaa taggcgccgt gctgcctggc cagcgcgaac gcctcctcgt
     4861 cggccaggct cgcgtgctcg atggtgtcca cgcctgcgcg cagcgcatct ttgatgcccg
     4921 cggtgccgtg agcatgcacg gccactttca gtccaagcat gtgagcttca tccaccagcg
     4981 ccttcatctc ggtcaggtcg tattgctggc caccgacgct atcggtcttc gacagcacgc
     5041 ccccggtgcc acagaacttg attacctccg caccgtattt gcgcagcttg cgcaccattg
     5101 cccgaatgtt ctccggacca ttcgcaatat tggtgtcagg cgtggtaatc gaaggaggaa
     5161 actcggtgtc atcgcaatgc ccaccggtcg ccccgatcgc ataggtcgca ggaacgatgc
     5221 gcggaccttg gatgtacccc tgctcgatcc cctgcttgag tgccacatcg tcgaaattgg
     5281 ccgagccgac attgcgcacg gtcgtaaacc cggcgtccaa ggttcgcttt gcgttggcga
     5341 caccgacgac ggtccaaaag ttgtcggtga attccaggcc gcggtagcca ctgaaatgcg
     5401 gatcaatcgt cagatggacg tgcatatcga tcaggcccgg caacaaggtc atcccggaga
     5461 gattgacgcg gcgcacgtca gcggttggcg ccgtggcggc atcttttgac cccacgctaa
     5521 cgatgcggcc gtcggtgatc gtgatctgcg gatgatcgac cactcggcct gcgaccacat
     5581 cgagcatccg atcagcggtc acgatgaccg tatcggcaga tgctgcgaac gagatgaaca
     5641 ggactgcgca gcccagccac gacaggtgag gcttattcat cagacactcc cggaaactcg
     5701 tacccaacgc ctgtcagttg tccatttttt cgatcgcgtc gacgcggtca ggataaaagg
     5761 caagatagtg cttgatcgtc gcgacggcag gatagggatc ctcataggtc cataccgcat
     5821 tgatggaccg gtctttgcct aggggaatgc tgtagtagca acactcgccc ttgtaaggac
     5881 agtacgttga gtgcgcggtg cgcatcagca aactcatatc cacgtcctgg cgaggaaagt
     5941 agtgaacgtc cggataggtc gcctccctca gcaccacggc tgcgcgcgtg tccgcgacca
     6001 aacgg
//
LOCUS       DATMRM010000153         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1016718, whole genome shotgun sequence.
ACCESSION   DATMRM010000153 DATMRM010000000
VERSION     DATMRM010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016718"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..862
                     /gene="rfbC"
                     /locus_tag="VNZ06_04515"
     CDS             314..862
                     /gene="rfbC"
                     /locus_tag="VNZ06_04515"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HWW20046.1"
                     /translation="MQFEPTRLPEVVLIQPRVFGDERGFFFETWQQRTFAKAGISAAF
                     VQDNHSRSSRYALRGLHFQIQQPQGKLVRVTRGEAFDVAVDIRRSSPRFGQWVGVLLS
                     EANHRMLWVPPGFAHGYLALSDSIDLVYKCTDFYAPQHERAIRWDDPAIGLQWPLPAG
                     VSPILSAKDAVAPSLREVETYP"
     gene            859..1746
                     /gene="rfbD"
                     /locus_tag="VNZ06_04520"
     CDS             859..1746
                     /gene="rfbD"
                     /locus_tag="VNZ06_04520"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HWW20047.1"
                     /translation="MRVLITGASGQVGSALYRSAPPGSQLQAMTRAELDIRDAPAVEA
                     AVRAFAPQVIINAAAYTAVDRAEEESQLATAINAHGPAALARAAHARPGCRLLHISTD
                     YVFDGRATRPYQPQDRPNPLSVYGRSKLQGEQAVTEQLGERAVILRTAWVYAAEGKNF
                     LLTMLRLMRERGAVRVVADQLGTPTAADSVARALWVVASRPDIRGILHWTDAGTESWC
                     GFATAIAQEAQAAGLVSVIPQVTAVATADYPTRAHRPANSVLDTASTSTLLGITPCPW
                     RTNLRATVAMIASAGRTSS"
     gene            1743..>2463
                     /gene="rfbB"
                     /locus_tag="VNZ06_04525"
     CDS             1743..>2463
                     /gene="rfbB"
                     /locus_tag="VNZ06_04525"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HWW20048.1"
                     /translation="MNRLLITGGAGFIGANFVHYWLGTHAGDRVVVLDALTYAGNLSN
                     LQSIAQRAEYRFVHGNICDTPLVERLLREERLDTIVHFAAESHVDRSIEGPDAFIETN
                     VIGTHSLLKAARRVWLDERHVPTHRFHHVSTDEVYGSLGPTDAAFTESTAYAPNSPYS
                     ASKAASDHLVRAYHHTYGLCTTISNCSNNYGPLQFPEKLIPLMITQILAGKSLPVYGD
                     GRNIRDWLHVEDHCRGIETVLR"
BASE COUNT          434 a          813 c          772 g          444 t
ORIGIN      
        1 cgccgctgtc gcggcactgt gatagcccgc ggcgacgcga aaacccgccg cggcgagtct
       61 cacgcagatg gcctggccga tgccgccccc ggcgccagtc accatcgcga ccggagagct
      121 cgtggattgc gtcatgctca tgcctggcag tatagcgcct cgcgccaagc gggcaggatc
      181 catgggcgtg ataagctggt gccgagcctg accctcccgt tgctgcacgc ggcgtgcgtc
      241 ttgccgacct gcgccggcta gcggcaggct gttcctttgt tgacgatatt gacgatgcga
      301 aatagggagc cacatgcaat ttgagccaac ccgacttccc gaagtcgtgt tgatccagcc
      361 gcgggtattc ggtgacgagc gcggattctt cttcgagacc tggcagcagc gcactttcgc
      421 caaggccggt atcagcgcag cgttcgttca ggacaatcac agccgctcga gccgatacgc
      481 cctgcggggt ctgcattttc agatccaaca gccgcagggc aagctggtgc gcgtgacgcg
      541 cggcgaggcc ttcgacgtcg cggtggacat tcgccgcagc tcgccgcgct tcggacaatg
      601 ggtgggcgtg ctgctgagtg aggccaatca tcgcatgctg tgggttcctc ccggctttgc
      661 gcacggctac ctggcgctga gcgacagcat cgatttggtc tacaagtgca ccgacttcta
      721 cgcgccccag cacgagcgtg cgatccgctg ggacgatccc gccatcggcc tgcagtggcc
      781 gctgcccgct ggcgtctcac cgatcctatc cgccaaggac gcggtggcgc cgagcttgcg
      841 ggaagtggaa acctatccgt gagagtgctg atcaccgggg cttccggtca ggtcggtagc
      901 gcgctgtatc gaagtgcccc cccgggctcg cagctacagg ccatgacgcg cgcggaactg
      961 gatatcaggg atgcgccggc cgtggaggcc gcggtgcgcg cctttgcgcc ccaggtcatc
     1021 atcaatgccg ccgcctatac tgcggtcgat cgcgctgaag aggaatcgca gcttgccacg
     1081 gcgatcaatg cacacggacc ggccgcgctg gcccgcgcgg cccacgcccg cccgggctgt
     1141 cgcctgctgc atatctcgac cgactacgta ttcgatggac gcgccaccag gccctaccag
     1201 cctcaggatc ggcccaatcc cttaagcgtc tacggtcgca gcaagctcca gggtgaacag
     1261 gccgtgaccg aacagctggg cgagcgcgcc gttattctgc gcaccgcgtg ggtctatgct
     1321 gccgagggca agaactttct gctcacgatg ctgcggctga tgcgcgaacg cggtgcggtg
     1381 cgcgtcgtcg ccgaccagct cggtacgccg accgcggccg attctgtcgc ccgggcgctg
     1441 tgggtcgttg cttcgaggcc agacattcgc gggatcctgc attggacgga tgccgggacc
     1501 gaaagctggt gcggcttcgc gacagcgatc gcgcaagagg ctcaggcagc aggattggtg
     1561 tcggtcatac cgcaggtcac ggccgttgcc accgccgact atccgacgcg cgcgcatcgg
     1621 cctgccaaca gcgtgctcga cacggcctcg accagcacac tgctcggaat caccccatgc
     1681 ccgtggcgaa ccaatctgcg cgcgaccgtg gccatgatcg catcggctgg caggacttcc
     1741 tcatgaatcg attactcatt accggcggcg ccggattcat cggcgcgaac tttgttcatt
     1801 actggctcgg cacgcacgcg ggcgatcggg tcgtggtgct cgatgcgctc acctacgccg
     1861 gcaacctgtc caacctgcag agcatcgcgc aacgagccga gtaccgcttt gtccacggca
     1921 atatctgcga tacgccgctg gtcgagcgcc ttctgcgtga ggaacggctc gatacgatcg
     1981 tgcactttgc agccgagtca cacgtggatc gatcgatcga gggacctgac gcattcatcg
     2041 aaaccaacgt gatcggcaca cactcgctgc tcaaagccgc gcgccgggtg tggctggacg
     2101 agaggcacgt gccgacgcat cgttttcacc acgtgtcgac cgacgaggtg tatggctcgc
     2161 tgggcccgac ggatgccgca ttcaccgagt ccactgccta cgcgccgaat tccccctact
     2221 ccgcgagcaa ggcagcctcc gatcatctgg tgcgcgcgta tcaccacacc tatgggctgt
     2281 gtacgacgat cagcaattgc tccaacaact atggaccgct gcagttcccg gagaagctga
     2341 tcccactgat gatcacgcaa atactggcgg gcaagtcact tccggtctat ggcgatggcc
     2401 gcaatatccg ggactggctg catgtggaag accattgtcg cggcatcgag acagtcctca
     2461 ggc
//
LOCUS       DATMRM010000154         5622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3481627, whole genome shotgun sequence.
ACCESSION   DATMRM010000154 DATMRM010000000
VERSION     DATMRM010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5622
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3481627"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..675
                     /locus_tag="VNZ06_04530"
     CDS             199..675
                     /locus_tag="VNZ06_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWW20049.1"
                     /translation="MQHYTADKYRAKTSVGYLVKRANALVIEGLEPALAEQGFTFTQW
                     VVMMHVRDGLAPNAKDICVQLRHDSGAVTRILDQLEARALLVRERSLDDRREVKLRLT
                     QAGHQMVESLIPTVVNKLNAVLIDFSSAEVTELTRLLNKFIAGLNSANSARPGEAA"
     gene            675..2111
                     /locus_tag="VNZ06_04535"
     CDS             675..2111
                     /locus_tag="VNZ06_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWW20050.1"
                     /translation="MSQFGSRITRWLTLTTATVLSACVTPPRGEPPALAPIEPSSLGL
                     NGQAVQPVDSSWYQSFGDQQLNRLITQALQNNPGLIEVATRLREAQAQASEARAARFP
                     SAKLSGGETHLKIPSGFPPALDAGHTVWAGDLGASLAWDLDLWGKHADAFAQAIALSR
                     AAGLDLDSARVLLTGSVVQTYIDLNRAYLRADIAERTEAQRSNILDITKQRVAAGLDT
                     RVELRQAEAQLLQARVALQQAQSAQALAAHELAALLGRGAADYAGITRPTLDLQAGLP
                     LPTALPINLLSRRPDVIAARERIDAADAGRRAAKAAFYPTVNLSAFAGFASFSLTDLI
                     SSPSFGYGAGPAVSLPLFDADRLRAQYRGTQAQLDGAVVAYDETVVRAVHQAADQLTL
                     IDALAGELELQRQSLAASEDAYRLAEERYRAGLAGYLTVLDVETEVLNEREQRVDLSH
                     SLALARVTLLLAVGGSFQNPNPISAAAR"
     gene            2149..3345
                     /locus_tag="VNZ06_04540"
     CDS             2149..3345
                     /locus_tag="VNZ06_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWW20051.1"
                     /translation="MDTPNNASNDRRRKIGFAILGVIVLVGAIAWFARWFLISSHYQS
                     TDDAYISSDLVQITSEIPGTVLAVRVDDTQSVERGTALVELDPADARVAANSAEAQLA
                     RAVRNVRGLFAQRDQLQAQIAQRESDVKRAQDDVSRREGLLSDGAVSGEELSHVRDAL
                     TASRAALDGARQQLNATEAQISGTTIETHPQVLDAEAAVRNVALTLHRTQLRAPVAGV
                     IVKRSVEVGQRVAAGAPLMGLVPLDDVWVDANFKEVQLRYIRVGQPVSLHADLYGSHV
                     EYHGKVAGLSAGSGSAFALLPAQNASGNWIKIVQRIPVRIVIDPQDLKDHPLRVGLSM
                     SVEVNVQDASGPLVATQVRSTPLPTQASAGDDPDVEQRIRDIVVQNAGHTPQGSLARA
                     VAVTGR"
     gene            3342..4877
                     /locus_tag="VNZ06_04545"
     CDS             3342..4877
                     /locus_tag="VNZ06_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWW20052.1"
                     /translation="MSCCGDITGAKRALVALALAFGTFMQVLDTSIANVAIPTIAGNL
                     GVSTDQGTWVITSFAVSNGICVPLTGWLMQRYGAVKTFAVSVLAFTVASLLCGISWSL
                     PSLVVFRVLQGAVSGPMIPGSQALLLMLFPPERKGTALAIWSITTLVAPVTGPVLGGY
                     VSDNWTWPWIFYINIPVGVFASTVCYRYLKHYETPTRRLPIDTVGLGLLALWVGSLQI
                     MLDKGKDLDWFNSTPILVMTLVAAVTFVAWLIWELTERYPMVDLSLLKYRNFRIGTIG
                     YCLGYAVFFGNIVLFPLWLQTQLGYTATWAGLVAAPAGVISILISPLVGRFVGKSDAR
                     WLASIGFLSFAASYFLRARLTADASFFEFILPQLVLGVGMGTFFISLLAISLDGLPAD
                     RVPAASGLGNFLRTVAAAFATSITTTFWDRHESLHQTHLAESSSLFSPTLQSALSGLQ
                     SAGINDPTVAAGAITQGLVHQAYLLSSLDYFWISGWITLLPVALLWFAHKPKGAGPAV
                     AAE"
     gene            4942..>5622
                     /locus_tag="VNZ06_04550"
     CDS             4942..>5622
                     /locus_tag="VNZ06_04550"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWW20053.1"
                     /translation="MPHLKKAALTAAASCLLACLAGPVTAQEHTDMASNIGAAKLIIG
                     DVKQNQTYFEQMFGMKEVNHYRSKDLYDEPIMGFEEGARLALFSPLADAPLKKSQYPV
                     ALLYTPDLDGVVKRLQDANYPVRRLDAANAGTVQIAIARDPSGNAIELVARPGKWEVG
                     GSKLIVNDRQKAEDFFVKVFGLSVGRRFQTPAYDEVVMSFGSGAVLALFQPLSEAPLP
                     KSQYPVVAI"
BASE COUNT          968 a         1762 c         1808 g         1084 t
ORIGIN      
        1 ctcgggcaag ccgatccgct taaatgcttg ctgctgacaa aatccgacct gcccatcgcg
       61 gatactcata tcagaatcgc tatttgctga aggctgagat tcgatgatcg aaatagttga
      121 cacaggcagg gatctgtcgc caaatgcctg ccaaagcaga cactgcgaat gcagtcagta
      181 tcaggacggt aactttctat gcaacattac acggcagaca agtaccgagc caagaccagc
      241 gtgggttacc tcgtgaagcg ggccaacgcg ctggtgatcg aaggacttga gccggcgttg
      301 gccgagcaag gtttcacgtt cacccagtgg gtcgtgatga tgcacgtgcg cgatggtctg
      361 gcgccgaacg ccaaggatat ctgcgtgcag ctgcgtcacg acagcggagc ggtcactcgc
      421 atcctggacc agctggaagc gcgcgcgctg ctggtacgcg agcgtagcct ggatgatcgc
      481 cgcgaggtga agctgcgatt gacgcaagcc ggccaccaga tggtcgagtc gctgatccca
      541 accgtggtca acaagctcaa cgcggtgctg atcgacttct cgagcgccga agtgacggag
      601 ctgacgcgcc tgctcaataa attcattgcg ggtctgaaca gcgccaacag cgcgaggccc
      661 ggggaggcag cctaatgagc cagttcggtt caagaataac gcgctggctc acgctgacca
      721 cggcgaccgt gctgtccgca tgcgtgacgc caccgcgtgg cgagccgccc gcactcgcgc
      781 cgatcgagcc gtccagtctg ggactgaatg gtcaagccgt gcagccggtc gactccagct
      841 ggtatcagag tttcggcgat cagcagctca atcggctcat cacgcaggcg ctgcagaaca
      901 accccggtct gatcgaagta gccacgcgtt tgcgcgaggc gcaagcgcag gccagcgaag
      961 cgcgtgcggc tcgtttcccc agcgccaagc tcagcggggg cgagacccac ctgaagattc
     1021 cttccggctt cccgcccgcg ctcgatgccg gccacacggt ttgggcaggc gatctgggcg
     1081 cttcgctggc atgggatctg gacttgtggg gcaaacatgc cgacgcgttc gcccaggcga
     1141 tcgccctgag ccgcgctgcc ggactcgacc tggacagcgc gcgcgtgctg ctgaccggtt
     1201 cggtggtgca gacctacatt gatctgaacc gggcctatct gcgagccgat atcgccgagc
     1261 gtaccgaggc gcaacgctcc aacatcctgg atatcacgaa gcagcgcgtc gcggcgggac
     1321 tggatacgcg cgtcgaactg cgtcaggccg aggcgcagct cttgcaggcg cgtgtcgcgc
     1381 ttcagcaggc gcaatccgcg caggcgctgg cggcgcatga actggcggcg ctgctcgggc
     1441 gcggtgccgc ggactacgcc ggtatcaccc ggccgaccct cgatctgcag gctggactgc
     1501 cgctgccgac ggcactgccg atcaacctgc tgtcgcgtcg tcccgatgtc atcgccgcgc
     1561 gagagcgtat cgatgcggcc gatgcgggca ggcgcgccgc caaagcagcc ttctatccaa
     1621 ccgtgaacct gagtgccttc gcaggctttg cctcgttcag tctcaccgat ctgatcagct
     1681 cgccgtcgtt tggctacggc gctgggcctg ccgtctcgct gccgttgttt gacgccgatc
     1741 gtctgcgtgc acaataccgc ggcacgcagg cgcagcttga cggtgccgtc gtggcgtatg
     1801 acgagacggt ggtgcgtgcc gtgcatcagg cggccgacca gctgacgttg atcgatgcgc
     1861 tggccggaga actcgagctg cagcgacagt cgctggcggc ttccgaagac gcctatcgcc
     1921 tggccgagga gcgttatcgg gccggtcttg ccggctatct gaccgtgctc gacgtcgaga
     1981 ccgaagtcct caacgaacgc gagcagcgtg tggaccttag tcattcgctg gcgctcgcac
     2041 gcgtcacgct gttgctggcg gttggcggca gtttccaaaa tccaaatccg atcagcgctg
     2101 cggcgcgcta gcgccgcgac cccattctac gacctcaagg caattctcat ggatacccca
     2161 aacaacgcct ccaatgaccg cagacgcaaa atcggctttg cgattctcgg tgtgatcgtg
     2221 ctcgttggcg cgattgcctg gttcgctcgc tggttcctga tctccagtca ctaccaaagc
     2281 acagatgatg cttacatcag cagtgacctg gtacagatca ccagcgagat accgggtacc
     2341 gtactggccg tgcgggtcga tgacacgcag agcgtcgagc gcggcactgc attggtcgag
     2401 ctcgatccgg cggacgcgcg ggtggcagcc aattccgccg aggcgcagct cgcgcgcgcg
     2461 gtgcgtaacg tgcgcggact gttcgctcag cgcgatcagt tgcaggcgca gatcgcgcaa
     2521 cgtgaatccg acgtcaagcg cgcccaggac gacgtcagcc gacgcgaggg tctgctgtcc
     2581 gacggggcgg tgtcgggcga agagctgtcg cacgtgcgcg atgcgctgac agcctcgcgt
     2641 gccgccctgg atggcgctcg ccagcagctc aatgcgaccg aggcgcagat ttccggtacg
     2701 accatcgaaa cccatccgca ggtgctcgat gctgaggccg ccgtgcgtaa cgtcgcgttg
     2761 acgctgcatc gtacgcagct gcgcgcgccg gtcgcgggcg tgattgtcaa gcgctcagtc
     2821 gaagtcgggc agcgtgtggc ggccggggct cccctgatgg gactggtgcc gctcgatgac
     2881 gtgtgggtcg acgccaactt caaggaagtc cagctgcgct acatccgcgt cggccagccg
     2941 gtcagcctgc acgcggatct gtacggcagc catgtcgagt atcacggcaa ggtcgcgggg
     3001 ctgtccgccg gcagcggcag cgcgttcgcg ttgctgccgg cgcagaacgc gtcgggcaac
     3061 tggatcaaga tcgtacagcg cattccggtg cggatcgtca tcgatccgca ggacctgaag
     3121 gatcatccgc tgcgcgtcgg cctgtcgatg agcgtggagg tcaatgtgca ggatgccagc
     3181 ggcccgctgg tggcaactca ggtgcgcagc acgcctctgc cgacgcaggc cagtgccggc
     3241 gatgatcctg acgtggaaca gcgcatccgc gacatcgtcg tccagaacgc cggacacacc
     3301 cctcagggat ccttggcgcg cgccgttgcg gtaaccggtc gatgagctgc tgtggcgaca
     3361 tcaccggcgc gaagcgcgcg ctggtcgcgc tggcgttggc gttcggcacc ttcatgcagg
     3421 tgctggacac gagtatcgcc aatgtcgcca ttccgacgat cgccggcaac ctgggcgtct
     3481 caacggatca gggtacctgg gtcattactt cgttcgcggt cagcaatggc atctgcgtac
     3541 cgctgaccgg ctggctgatg caacgctatg gcgccgtgaa gacctttgcc gtatcggtcc
     3601 tggcgttcac tgttgcctcg ctcctgtgcg gtatctcctg gagtctgccg tctctggttg
     3661 tatttcgggt cctgcagggc gcggtgtccg gtccgatgat tccgggatcg caggcactgc
     3721 tgctgatgtt gtttccaccg gagcgcaagg gcacggcgct cgctatctgg tcgatcacca
     3781 ctctggttgc gccggtcacc ggtcccgtgc tcggcggata cgtctccgat aactggacct
     3841 ggccctggat tttctacatc aacattccgg tgggagtgtt cgccagcacg gtgtgctacc
     3901 ggtatctcaa gcattacgag acgcccacgc gtcgcctgcc gatcgatacc gtggggctgg
     3961 gactgctggc gctgtgggtc ggctcgctgc agatcatgct cgacaagggc aaggatctcg
     4021 actggttcaa ttccacaccg atcctcgtca tgaccctcgt cgcggcggtt acattcgtgg
     4081 cctggctcat ctgggagttg accgagcgct atccgatggt ggacctgtcg ctgctcaagt
     4141 accgcaactt cagaattggc acgatcggct attgtctggg ttatgcggtg ttcttcggca
     4201 acatcgtgct gttcccgctg tggttgcaga cgcagttggg ctataccgcc acctgggccg
     4261 gactggtcgc tgccccggcc ggcgtcatct ccattctcat ctcgccgctg gtggggcgtt
     4321 tcgttggcaa gtcggacgca cgctggctcg cgagcatcgg ctttctgtca tttgccgctt
     4381 cgtatttcct gcgggcccga ctcaccgccg atgccagctt cttcgagttc atcctgccgc
     4441 agctcgtgct cggcgtgggt atgggaacct tcttcatctc attgctggcg atttcgctcg
     4501 acgggctgcc ggccgaccgc gtgccggcgg cctccggact ggggaacttc ctgcgcacgg
     4561 tagccgcggc gttcgcgact tcgattacga ctaccttctg ggatcgtcac gagtccctgc
     4621 accagaccca tctggcggag agttccagcc tcttcagccc gacgctgcag tcggccctga
     4681 gtggtctgca atccgccggg atcaacgatc cgaccgtcgc cgccggtgcg attacccagg
     4741 gcctggtgca tcaggcgtac ctgctgtcct cgctcgacta tttctggatc tcgggctgga
     4801 ttactttgct gccggtggcg ttgctgtggt tcgcccacaa gccaaaggga gcaggtccgg
     4861 cggtcgccgc cgagtgagca cccaggctgg gtctttagta tgatccgggt ttactaacag
     4921 catcgacggg ggacgcgcga catgcctcat ctgaagaagg ccgccctgac ggcggccgca
     4981 agctgtctgc tggcatgctt ggcgggcccg gtcaccgcgc aggagcacac cgacatggct
     5041 tccaacattg gcgctgccaa gctgatcatc ggcgacgtca agcagaacca gacctatttc
     5101 gagcagatgt tcggcatgaa ggaggtcaat cactaccggt ccaaggatct ctacgatgag
     5161 cccatcatgg gtttcgagga gggcgctcgc ctggcgctgt tctcgccgtt ggccgacgcg
     5221 ccgctcaaga agagccaata tccggtggca ctgttataca cgccggatct ggacggcgtc
     5281 gtcaagcgcc tgcaggacgc gaactatccg gtgcggcgcc tggatgccgc caatgcgggg
     5341 actgtgcaga tcgcgatcgc ccgcgatccg agcggcaatg cgatcgagct ggtcgcaaga
     5401 ccgggcaaat gggaggtggg tggctcgaag ctgatcgtca acgaccgaca aaaggccgag
     5461 gatttcttcg tcaaggtatt cggactgagc gttggccgac gcttccagac acccgcctac
     5521 gacgaggtgg tgatgagctt cggctccgga gcggtcctgg ccctgtttca gccgctgtcg
     5581 gaagcaccgc tgccgaagag tcagtacccg gtggttgcga tc
//
LOCUS       DATMRM010000155        25603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3178674, whole genome shotgun sequence.
ACCESSION   DATMRM010000155 DATMRM010000000
VERSION     DATMRM010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 25603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25603
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3178674"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1291)
                     /locus_tag="VNZ06_04555"
     CDS             complement(35..1291)
                     /locus_tag="VNZ06_04555"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWW20054.1"
                     /translation="MTTEIRNLTLNFGPQHPAAHGVLRLVLEMDGETIQKADPHIGLL
                     HRGTEKLAESKPFNQSIGYMDRLDYVSMMCNEHAYVLAIEKLLGIQPPPRAQYIRTMF
                     DEITRILNHLMWLGAHGLDIGAMTVFLFCFNQREDLMDCYEAVSGTRMHATYYRPGGV
                     YRDLPEVMPKHQPSRWRSQKDTDRRNELRTGSLLDFIEAFTQTFLGHVDEYETLLTDN
                     RIWKQRTVNIGVVTPERAMQLGFTGPMLRGSGIEWDLRRKQPYERYSELEFDIPVGVN
                     GDCYDRYLVRVAEMRQSNRIVQQCVKWLRANPGPVMVDDRKVRAPSREHMKGDMESLI
                     HHFKFMTEGYCVPEGESYAAVEAPKGEFGVFLVSDGANKPYRLHCRAPGFVHLASLGE
                     MVKGHMLADVVAVIGTQDIVFGEVDR"
     gene            complement(1288..1986)
                     /locus_tag="VNZ06_04560"
     CDS             complement(1288..1986)
                     /locus_tag="VNZ06_04560"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWW20055.1"
                     /translation="MPAYIDELAAKIKAVLGERARPYAAPSGEYAIEVDSARYVEVAR
                     TLRDDPQLSFEQLIDLAGVDYLDYGRAEWRTTAATASGFSRGVNHAAPHTLAGNKRFA
                     VTSQLLSISHNQRLRVRVWADSVEDPMLDSLVGVWASAEWYEREAFDLFGILFRGHPD
                     LRRILTDYGFIGHPFRKDFPLIGNVEVHYDEKLKRVVYGPVEIEPRILVPRVIRHDNR
                     YDPALHDHPPGDRA"
     gene            complement(2003..2542)
                     /locus_tag="VNZ06_04565"
     CDS             complement(2003..2542)
                     /locus_tag="VNZ06_04565"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWW20056.1"
                     /translation="MEERSARVGLEAPDSQGFAQRGFLTTSVDNLVNWARTGSLWPMT
                     FGLACCAVEMMHAGASRYDLDRFGVTFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQM
                     PEPRWVISMGSCANGGGYYHYSYAVVRGCDRIVPVDVYVPGCPPTAEALLYGVIQLQR
                     KIERHSIAAAAKARPEVIA"
     gene            complement(2512..2880)
                     /gene="ndhC"
                     /locus_tag="VNZ06_04570"
     CDS             complement(2512..2880)
                     /gene="ndhC"
                     /locus_tag="VNZ06_04570"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWW20057.1"
                     /translation="MLTNYLPVLIFLGIASALAAVLLGLGTLLGRLSASYRNDRAKVS
                     PYECGFEPFEDSRRRFDVRYYLVAILFIIFDLEIAFLFPWAVALKRIGTFGLATMGIF
                     LLILVIGFAYEWKKGALEWD"
     gene            complement(2975..3059)
                     /locus_tag="VNZ06_04575"
     tRNA            complement(2975..3059)
                     /locus_tag="VNZ06_04575"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3023..3025),aa:Leu,seq:gag)
     gene            complement(3094..3564)
                     /gene="secG"
                     /locus_tag="VNZ06_04580"
     CDS             complement(3094..3564)
                     /gene="secG"
                     /locus_tag="VNZ06_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HWW20058.1"
                     /translation="MIIHFFCAASIVALVLLQRGKGAEAGAGFGAGASGTVFGARGAS
                     TALSRATAILAAIFMVTSLSLAYVGTRKAATPSSGVLEQFAQPAAPAKGTPGAPPATV
                     TLPAPAPAAGAAAPTSTLPAQSAPPAAATHSAPAAQSAPPATTPTAPANPTAPK"
     gene            complement(3596..4348)
                     /gene="tpiA"
                     /locus_tag="VNZ06_04585"
     CDS             complement(3596..4348)
                     /gene="tpiA"
                     /locus_tag="VNZ06_04585"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWW20059.1"
                     /translation="MRRKLVAGNWKMHGSRTDNSALLDAIVAAPKLESVECVVCPPYV
                     YLSEIARLLRGTAVKLGAQNVSAETHGAYTGEVSAAMLKDVGCQFVIVGHSERRTLSH
                     ESDEQVARKFAAAQSKGLVPILCVGEQLPEREAGRCFDVVGRQLEAVVALTGISSFAH
                     AVIAYEPVWAIGTGRTASPAQVQEIHGFIRARLTQRDAKIAAGLQILYGGSVKAGNAR
                     ELFTQADVDGGLIGGASLKAEEFLSIVAAARG"
     gene            complement(4447..5799)
                     /gene="glmM"
                     /locus_tag="VNZ06_04590"
     CDS             complement(4447..5799)
                     /gene="glmM"
                     /locus_tag="VNZ06_04590"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004639966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWW20060.1"
                     /translation="MSRTYFGTDGIRGRVGTDPMTVEFALRLASAAAQVLLREGGAVL
                     IGKDTRVSGYMFEAALEAGFVAAGADVLMTGPLPTPAVAYMTRQMQCRLGVVISASHN
                     PYEDNGIKFFDGEGGKLSDAIEERIEAQLVSVPLTRASREIGRARRVDSALFHYQEFC
                     NRMLGAGNNLSGLKLVIDCANGAGYKIAPRILADLDADIVPIGCSPNGRNINDGCGST
                     SPELLQLTVPGVHAHVGVALDGDGDRVMMVDHLGRLVDGDQLLYVIASAEKAEQRLRG
                     PVVGTVMSNFGLELALRRLGIEFLRAPVGDRHVLAKLKESGGILGGETSGHILSLDRT
                     TTGDGLITALQVLRIMKQTGRSLAELAAGMEKLPQVLLNVRVARRFDPMSVPDVVNAV
                     RRVEKQLHGEGRVVLRASGTEPVIRVMVEARDATEVRACAEELAAVVRGAPAPAPAPT
                     "
     gene            complement(5796..6617)
                     /gene="folP"
                     /locus_tag="VNZ06_04595"
     CDS             complement(5796..6617)
                     /gene="folP"
                     /locus_tag="VNZ06_04595"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWW20061.1"
                     /translation="MDLSRPLVMGVLNVTQDSFSDGGKFLDPDAAFAHAERMSSEGAA
                     IIDIGGESTRPGAAGGVSSEEELRRVLPVVERIAARLKVLISVDTSNPELMSRAPAAG
                     AHLINDVRALQRPGALEAAASTPCGVCLMHMLGEPHSMQVDPHYQDVVKEVRAFLQDR
                     VQACVDAGIARERLCVDPGFGFGKRLEHNLQLLRKLPQLESLHLPVVVGLSRKSMLAT
                     LLGRKGDAGSDRLAASLALATAAVLQGAHIVRAHDVAQTVDAVRVASAIHYGSPP"
     gene            complement(6696..8624)
                     /gene="ftsH"
                     /locus_tag="VNZ06_04600"
     CDS             complement(6696..8624)
                     /gene="ftsH"
                     /locus_tag="VNZ06_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013974289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HWW20062.1"
                     /translation="MNDLTKNILLWVVIVLVMLAVFSRYVPNGTQAQAINYSEFYNDV
                     KAGRVESAVLQGDTIHGTLKDKTHFETYNPETNYSVLIGDLLKAGVSLEGQPPKQPNF
                     LAQLVLQLAPAMLLLVVFVYLLRQMQGAAGGRGAMSFGKSRARLLGEDQVNVTFADVA
                     GVDEAKQEVGEIVDFLKDPGKFQKLGGKIPKGVLMVGSPGTGKTLLARAIAGEAKVPF
                     FTISGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDER
                     EQTLNQLLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDVRGREQ
                     ILRVHMRRVPLSDDVKPALIARGTPGFSGADLANLVNEAALFAARANQRLVTMDQFER
                     AKDKIMMGAERRSMVMTDAEKRMTAYHEAGHAIVGITVPEHDPVYKVTIIPRGRALGV
                     TQFLPEQDRYSMSKRRLESSIATLFGGRIAEEIIFGVDSVTTGASNDIERATELARNM
                     VTKWGLSDRLGPLTYSEDQGEVFLGRSVTQHKQVSDETAHAIDEEVRRVIESNYKVAR
                     EILDTNIEKLHAMAEALIKYETLADDQLKDIMAGRAPRPPDGWDDSTPSPGGGKPVPS
                     PLAGPVEPPVVRPAANQH"
     gene            complement(8684..9310)
                     /gene="rlmE"
                     /locus_tag="VNZ06_04605"
     CDS             complement(8684..9310)
                     /gene="rlmE"
                     /locus_tag="VNZ06_04605"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HWW20063.1"
                     /translation="MARRSKSSDRWLREHFSDPFVKRARAEGWRSRAVFKLEQIDRKE
                     RLLKPGMVVLDLGAAPGAWSQYARQRVGKPGRVLASDILEMEPLAGVEFLQGDVREQV
                     VLDALKRAIGAPGIDLLMSDMAPNLSGMDVIDAPRSMYLSELALDMARETLKPGGTAL
                     IKVLQGAGFEALMGSARTSFGRVKCLKPEASRSRSSETYLLASGKRMV"
     gene            9339..9641
                     /gene="yhbY"
                     /locus_tag="VNZ06_04610"
     CDS             9339..9641
                     /gene="yhbY"
                     /locus_tag="VNZ06_04610"
                     /inference="COORDINATES: protein motif:HMM:NF014087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HWW20064.1"
                     /translation="MELSEKQLRFLRGRAHALKPVIQVGQKALTTGVIAETRRALQDH
                     ELIKLRIQASDRDALDALLAELVSAAEATLVARIGHVAVLFKPNPKLSRIPLPAKN"
     gene            complement(9670..10146)
                     /gene="greA"
                     /locus_tag="VNZ06_04615"
     CDS             complement(9670..10146)
                     /gene="greA"
                     /locus_tag="VNZ06_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HWW20065.1"
                     /translation="MKRSPMTLRGAELLRTELKRLKSEDRPRVIKAIAEARSHGDLSE
                     NAEYHAAREQQGFIEGRIGEIESRLGAAEIIDVASLPATGRVVFGATVELEDQAEGTG
                     VTYQIVGEDEADIRGGRISVTSPIARALIGKKQGDVVDVAAPGKTRSYEIVSVKYI"
     gene            complement(10143..13436)
                     /gene="carB"
                     /locus_tag="VNZ06_04620"
     CDS             complement(10143..13436)
                     /gene="carB"
                     /locus_tag="VNZ06_04620"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWW20066.1"
                     /translation="MPKRTDLNSILIIGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPETADSIYIEPINWRTVARIIERERPSALLPTMGGQTALNTALDL
                     VREGVLQQFGVEMIAASREAIDMAEDRERFRNAMREIGLEVPTSQIARSLEEALAVQS
                     SIGYPIVIRPSFTLGGSGGGIAYNREEFETIVQRGLDASPTRELLLEEAVLGWKEFEM
                     EVVRDRNDNCIIVCSIENVDPMGVHTGDSITVAPAQTLTDKEYQRMRDASIAVLRKIG
                     VECGGSNVQFAIDPVTGRMLVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LRNDITGGATPSSFEPTIDYVVTKIPRFTFEKFPAANTRLTTQMKSVGEVMAIGRTFQ
                     ESLHKALRGLETGLDGLDPMLTLPLTEETERQLNYELRAPGPNRLLYVADAFRAGWTV
                     ARVAELSAIDPWFLAQVADLIAEETRARREGESALGIERLRSLKRKGFSDRRLATLTR
                     SNEGAIRKKRREFGIAPVYKRVDTCAAEFSTSTAYLYSTYEEECEAEPTKQRKVMILG
                     GGPNRIGQGIEFDYCCVHAALALREDGFETIMVNCNPETVSTDYDTSDRLYFEPLTLE
                     DVLEVIAKERPEGVIVQFGGQTPLKLSRALEEAGAAIIGTSPDSIDVAEDRERFQQLV
                     RRLQLKQPANASARKEEQAMMLAREVGYPLVVRPSYVLGGRAMEIVFNEDDLRNYMTR
                     AVQVSNDAPVLLDRFLDVAIEVDVDAVCDGQDVLIGGIMEHVEQAGVHSGDSGCSLPP
                     NSLSVELQNEMRQQTRKLARALNVVGLMNIQFAIQNGTVYLLEVNPRASRTVPFVSKA
                     TGVPIAKIAARVMTGRKLREMGVLEERVPRYYSVKEAVFPFIKFPEADPILGPEMKST
                     GEVMGTGRTFGEAYAKAQAASGVVLPRSGLALLSVRERDKAAAVGLARRLMAHGFTLV
                     ATRGTAAALKAAGVECRTANKVREGRPHIVDMIKNDEIDLIVNTTEGKQAILESNSIR
                     REAVHRRVTYYTTMAAALATCEALDHIDEVDVNRLQDLHKEVAIMPASQGSTGATTTL
                     REVRREVGT"
     gene            complement(13449..14630)
                     /gene="carA"
                     /locus_tag="VNZ06_04625"
     CDS             complement(13449..14630)
                     /gene="carA"
                     /locus_tag="VNZ06_04625"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HWW20067.1"
                     /translation="MTAPAVLALGDGSIFKGQSIGASGNTTGEVVFNTAITGYQEILT
                     DPSYARQIVTLTYPHIGNYGVTPEDMESSQIFASGLVVRDLSMIPSSWRAAESLGEFL
                     QRGKIVAIAGIDTRRLTRLLREKGAQSGCIMTGDRINEDAAVRAARAFPGLKGMDLAK
                     VVTTREPYQWNEGSVGREPLNLLRAQQRLHVVAYDYGVKRNILRVLSDQGCRITVVPA
                     TTSAQDVLSVNPDGVFLSNGPGDPEPCDYAIKAISELLATDVPVFGICLGHQLLGLAS
                     GAKTVKMKFGHHGANHPVQELSTGRVMITSQNHGFAVDESTLGDKLVATHRSLFDGSL
                     QGLARSDVAAFSFQGHPEASPGPHDLKPLFDNFIQLMVRRLQNQLKQLRANARKQSAE
                     G"
     gene            complement(14917..15747)
                     /gene="dapB"
                     /locus_tag="VNZ06_04630"
     CDS             complement(14917..15747)
                     /gene="dapB"
                     /locus_tag="VNZ06_04630"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HWW20068.1"
                     /translation="MTAAAAGARRTVIVGATGRMGGQLLRLIGQFPGLRLHGAVATED
                     SQFLGQDAGRYAGVEPCGIAISASLPPLLREAELILDFSHPSAAAANLAACVAARVAL
                     LIGTTGLPRELDAPLAAAADSIALLVAPNTSLGVNLLMELVRQAAQGLPPGYDIEIVE
                     THHRAKRDAPSGTALALGAAAADGRAVSLSRQAVYARHGIGEPRQEDEIGFVSVRGGD
                     VVGEHEVWFLGEGERVLLKHSATDRAVFARGALLAGQWLAGRMAGRYAMRDVFISKFK
                     "
     gene            complement(15744..16889)
                     /gene="dnaJ"
                     /locus_tag="VNZ06_04635"
     CDS             complement(15744..16889)
                     /gene="dnaJ"
                     /locus_tag="VNZ06_04635"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HWW20069.1"
                     /translation="MATKRDYYKTLDLARNSTEADIKKAYRRLAMKYHPDRNPGDKEA
                     EEKFKEAKEAYEILSDAQKRSIYDQHGHAGIEASRNASAGGGGFQGAEGFGDIFGDVF
                     GDIFGGGRRGGASQVFRGADLRYEAELDLSEAVFGHTLEIDVKKLVECDTCGGSGAAK
                     GSNPVTCDTCGGNGQVRVSQGFFTLQQTCPHCRGSGRIVRNPCDACLGQGRVRRAKKL
                     SVKIPAGVDTGDRVRLGGEGEAGRNGGPPGDLYVEVHVREHPIFERDGVHLSCEVPIS
                     FATAVLGGKVEVPTLDGQVALTVPAESQSGRVFRLREKGVKSVRGGARGDLFCRCVVE
                     TPVNLSAEQRALLKQFDDSVKHDGSKHAPRQKSFLDGVKRFFSRDST"
     gene            complement(17008..18999)
                     /gene="dnaK"
                     /locus_tag="VNZ06_04640"
     CDS             complement(17008..18999)
                     /gene="dnaK"
                     /locus_tag="VNZ06_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HWW20070.1"
                     /translation="MSKVIGIDLGTTNSCVAIMEGGKPRVIENSEGDRTTPSIVAFTK
                     DDEVLVGQSAKRQAVTNPQNTTFAVKRLIGRKFEDEVVQKDIKMVPYKIVKADNGDAW
                     VEIRGKKMAPPEISARVLMKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLNVQRIINEPTAAALAYGLDKQGADRKIAVYDLGGGTFDVSIIEIAAVDGERQFEV
                     LATNGDTFLGGEDFDLRIINFLADEFLKESGVDVRRDPLAMQRLKEAAEKAKIELSSS
                     QQTDVNLPYITADASGPKHLNVKLTRAKLESLVEDLLQRTLGPCRTALKDAGLAASDV
                     AEVILVGGQTRMPLVQKYVKDFFGKEPRKDVNPDEAVAVGAAVQAGVLAGEVKDVLLL
                     DVTPLSLGIETLGGVMTKLIEKNTTIPTKAANTFSTAEDHQTAVTIHVLQGERERSGD
                     NKSLGKFDLSDIPPAPRGVPQVEVTFDIDANGILNVSAKDKATGKEQRIVIRASSGLA
                     EDEIKRMVKDAEAHAAEDKAFRELIETRNRADAMIHAVEKALKDLGDKVTADERGKTE
                     AALNDLKAVLKSDDKEKIEKKTEALGQASAGIAQRAYTANQGGAGAGDPPGGAAGPEA
                     SAAAGAGASGSAGGSQQKADENVVDAEFEEVRDKGRRAS"
     gene            complement(19092..19628)
                     /gene="grpE"
                     /locus_tag="VNZ06_04645"
     CDS             complement(19092..19628)
                     /gene="grpE"
                     /locus_tag="VNZ06_04645"
                     /inference="COORDINATES: protein motif:HMM:NF010748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWW20071.1"
                     /translation="MDQPEPQLEGSEEPLASGAAAELQSLRTALAAAQDATRSQRDQY
                     LRALAELDNVRKRAERDIESAHRYALERFVGELLPVRDGLELAARQGAQADAKTLLEG
                     QQATLKLLDRALDKARVKILDPLGDKFNPAQHEAVLMQPSETSEPDRVLQVVQTGYEL
                     NGRLLRPARVIVAKTNDG"
     gene            complement(19747..20814)
                     /gene="hrcA"
                     /locus_tag="VNZ06_04650"
     CDS             complement(19747..20814)
                     /gene="hrcA"
                     /locus_tag="VNZ06_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HWW20072.1"
                     /translation="MSQDSRDDALNERAQRLLRVLVQNYIRDGQPVGSRTLSRESGLT
                     LSSATVRNVMSDLEGLGFVTSPHTSAGRVPTDKAYRFFVDSLLRAQMPTDDASLEEIR
                     RQLDMPQDSSKALVAAASQLLSNITHLAGVVTMPLAHSAVLTQIEFLGLSENRVLVVL
                     VLDDQEVQNRIVQLERHYSSEELRRAANFLNEHCVGKPPAQIHQELVRQLKETHEHLN
                     QSMLGAIAMSQRLFEQGAAPSEDIEYVIAGETNLMGFSGVSDVDRLKRLFEAFNEKRD
                     ILHLLDQSLRAEGVQIFIGQESGYQILDAYSVVTAPYSSAKGVVGVLGVIGPTRMAYE
                     RVIPIVDLTAKVLGSALNSRR"
     gene            20984..21877
                     /locus_tag="VNZ06_04655"
     CDS             20984..21877
                     /locus_tag="VNZ06_04655"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HWW20073.1"
                     /translation="MQKPIKTCALVGRFQDARVAECAVPLISVLQSRQLKVLALQDST
                     APSAPAGVAIVPLAQLIEQAELIIAIGGDGTLLYAAGLVAEHDIPLLGINRGRLGFLT
                     DVLPQDMEGCIGAVLAGECEADSRPLLQARLRGSDGSEVQRFALNDVVFNRLETGRML
                     DFETRIDGRYVNSHGGDGLVVATATGSTAYALSCGGPIVNPKLNVLVVAPICPHTLSD
                     RPIVVPGESRVDIRLIGETPTTAQVTCDAAVLGELKTGGRLEIFPAGRSVTLLHPPGY
                     DYFRLLRSKLHWGLGGLTHER"
     gene            21884..23575
                     /gene="recN"
                     /locus_tag="VNZ06_04660"
     CDS             21884..23575
                     /gene="recN"
                     /locus_tag="VNZ06_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HWW20074.1"
                     /translation="MLRHLQIRDFAIIESVELDFSPGLTVLTGETGAGKSILVDALEL
                     LSGGRAEKDVIRAGADKADITATIDISKIAGELRHILEQQSITSDVDLMVRRVVSSDG
                     RTRAWLNGQSVPLQLVRSVTELLFDIHGQHEFQSLVRPATQRALVDTYGKLEALSGQV
                     RAAHSTWLALLNRSVELEAAASDRGSRLELLRHQVREIEALQIKPGETAQLKEERSRV
                     ANSSRLIEGVRASLECLFESDQASAHALLSKSLSSLRQASHLDPQLDPFAALLDEALI
                     RVTDASHSLQQYLDALDLDPQRGDAIERRLAAFEELARKHRISPDALLEKQSELTLQL
                     GGLEHAAADLTTVRTQLAAAGNSYHEIARQLSARRTTAARGLAKEISARMQELGMAGG
                     RFLIDVTQNEHAEPTANGLDQIEFRVSTNPGQPPRAIAKVASGGELARLSLAVQVSCA
                     ESGAGCMVFDEVDAGIGGAVAEIVGRELRALGERGQVLCVTHLPQVAAQGHQHLRVAK
                     KSEGKLTRTTVTPLSAEASVDEIARMLGGVEITARARAHAQEMLSKVPTSKRKSG"
     gene            complement(23572..24018)
                     /gene="fur"
                     /locus_tag="VNZ06_04665"
     CDS             complement(23572..24018)
                     /gene="fur"
                     /locus_tag="VNZ06_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HWW20075.1"
                     /translation="MEGKDLRRAGLKVTLPRLKILEILEHSATRHMSAEEIYKKLLDS
                     SEDIGLATVYRVLTQFESAGLVARHHFEDGMAVFEINHGTHHDHIVCMDCGAVEEFVD
                     PEIEAKQNSIATRLGYRIHEHSMVLYGRCQRVDCPAKKQGRVKVPS"
     gene            24046..24396
                     /gene="bamE"
                     /locus_tag="VNZ06_04670"
     CDS             24046..24396
                     /gene="bamE"
                     /locus_tag="VNZ06_04670"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HWW20076.1"
                     /translation="MRIIPLFALLGALSGCVYRMPIQQGNYLDPAAIAQVKVGMTHSQ
                     VRFLLGTPMVPGAFDDARWDYDYYLKIRRLRGPATGHATVYFQNNLVTRVISDVKEPP
                     MLPGLGRPIAPRPN"
     gene            complement(24424..24711)
                     /locus_tag="VNZ06_04675"
     CDS             complement(24424..24711)
                     /locus_tag="VNZ06_04675"
                     /inference="COORDINATES: protein motif:HMM:NF015611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HWW20077.1"
                     /translation="MKHCSVVCDTAAGLLACELELPDDATIDRALAAARDQLGEGATD
                     WEHTAIGIYGRLRVRSHVFADGDRIELYRPLQLDPRAKRRERALPHRGDKR"
     gene            complement(24708..25130)
                     /locus_tag="VNZ06_04680"
     CDS             complement(24708..25130)
                     /locus_tag="VNZ06_04680"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HWW20078.1"
                     /translation="MTRSALVARSPELVYQVVEDVERYPQFVPGCTEAKVLERSATEM
                     VARLAVRRGPLRTEFTTRNRLEPGRSMQMDLVDGPFRMLEGKWSFTPVASNGCRIELS
                     LRFQFSNLLKSALFEPLFEETAASLVRAFVIRAQSMPA"
     gene            25372..>25603
                     /locus_tag="VNZ06_04685"
     CDS             25372..>25603
                     /locus_tag="VNZ06_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HWW20079.1"
                     /translation="MAKAESPQRVIAENRRARFDYFIEERFEAGLALEGWEVKSMRAG
                     RAQLAEAYVYVRNGEAFLIGAHLSPLRTTSTHK"
BASE COUNT         4595 a         8567 c         7668 g         4773 t
ORIGIN      
        1 gtgaggagcg cactcttgcc gccttccgct gccatcaacg atcgacctcc ccgaacacga
       61 tgtcctgcgt accaatcacc gccacgacgt ccgccagcat gtgtcccttg accatctcgc
      121 ccagggacgc caggtgcaca aacccgggcg cccggcaatg caggcgatag ggcttgttgg
      181 cgccgtccga taccaggaac acaccgaact cgcctttcgg tgcttccacc gcggcgtagc
      241 tttccccttc cggtacgcag tagccctcgg tcatgaactt gaagtgatga atcagcgatt
      301 ccatatcgcc cttcatgtgc tcgcggctgg gcgcgcgcac cttgcgatca tccaccatca
      361 ccgggcccgg gttcgcgcgc agccatttga cgcactgctg gacgatgcga ttggactgac
      421 gcatctccgc cacgcgtacc aggtagcggt cgtagcaatc cccgttcaca cccaccggaa
      481 tatcgaactc gagctcggaa tacctctcgt agggttgctt gcgccgcaag tcccattcga
      541 tgcccgaacc acgcagcatc ggacctgtaa agccgagctg catcgcgcgt tccggggtca
      601 cgactccaat attgacggtg cgctgcttcc agatccggtt gtcggtcaaa agcgtctcgt
      661 actcatcgac gtgacccagg aaggtctggg tgaatgcctc gatgaaatcc agcagcgaac
      721 ccgtgcgcag ttcattgcgc cgatcggtgt ccttctgact gcgccagcgg gaaggctgat
      781 gcttcggcat cacttccggc agatcgcgat acaccccgcc cggccgatag taggttgcat
      841 gcatgcgtgt gcccgacact gcctcgtagc agtccatcag gtcttcgcgc tggttgaagc
      901 agaacaggaa caccgtcatc gccccgatgt ccaggccatg cgctcccagc cacatcagat
      961 gattcaggat ccgggtgatc tcatcgaaca tcgtgcggat gtactgcgcg cgcggcggcg
     1021 gctggatgcc gagcagtttc tcgattgcca gcacgtaggc gtgctcgttg cacatcatcg
     1081 acacgtagtc gagccggtcc atgtagccaa tcgactgatt gaacggcttg gactcggcga
     1141 gtttctcggt gccccggtgc agcaggccaa tgtgcggatc ggctttctgg atcgtctcgc
     1201 cgtccatctc cagcaccagg cgcagcacgc catgcgccgc cggatgctgc gggccgaagt
     1261 tgagcgtcaa attgcggatt tcggtggtca tgcccgatcc ccgggtgggt gatcgtgcag
     1321 tgccgggtcg tagcggttgt cgtggcggat cacgcgcggc accaggatgc gcggctcgat
     1381 ctcgaccggg ccgtacacga cacgcttgag tttctcatcg tagtgcactt ctacgttgcc
     1441 gatcagcggg aagtccttgc ggaacgggtg cccgatgaag ccgtagtcgg taaggatgcg
     1501 ccgcagatcc ggatggccgc ggaacaggat cccgaacagg tcgaacgctt cgcgttcgta
     1561 ccactctgcg ctggcccaca cgccgaccag cgaatcgagc atcggatcct cgacactgtc
     1621 cgcccacacc cgcacgcgca gccgctggtt gtgcgagatc gacaacagct gcgaggtcac
     1681 ggcgaagcgc ttgttgcctg cgagggtgtg aggcgctgcg tgattcactc cgcggctgaa
     1741 accgctggcg gtcgcggccg tggtgcgcca ctcggcgcgg ccatagtcga ggtagtccac
     1801 gccagccaga tcgatcagct gttcgaaact cagctgcggg tcgtcgcgca gcgtgcgcgc
     1861 cacctccacg tagcgtgccg aatccacctc gatcgcgtat tcaccgctcg gcgccgcata
     1921 aggacgcgcg cgctcgccta ggactgcctt gatcttcgcg gcaagctcat cgatataggc
     1981 tggcacgatg gggtggggtc cttcaggcaa tcacttcggg ccgtgccttg gcggcggcgg
     2041 cgatcgaatg acgctcgatc ttgcgctgca gttgaatcac gccgtacagc agtgcttcag
     2101 cggtcggcgg acagcctggc acgtacacgt ccacgggcac gatgcgatcg cagccgcgca
     2161 cgaccgcgta ggagtagtgg tagtagccac cgccattggc gcacgagccc atcgagatca
     2221 cccagcgcgg ctcgggcatc tggtcataga ccttgcgcag cgccggtgcc atcttgttga
     2281 ccagggtgcc tgcgacgatc atgacgtcgg actgacgcgg gctgggacga aacgtcacgc
     2341 cgaagcggtc gaggtcgtag cgcgaggcac cggcgtgcat catttccacg gcgcagcagg
     2401 ccagtccgaa ggtcatcggc cagagcgagc cggtgcgcgc ccagttgacc aggttgtcca
     2461 cgctcgtggt cagaaagcca cgttgcgcaa agccctgtga atcaggcgcc tctaatccca
     2521 ctcgagcgct cctttcttcc attcgtacgc gaatccgatg accaggatca acaggaagat
     2581 gcccatcgtg gccaggccga aggttccgat gcgcttgaga gcgaccgccc atgggaacag
     2641 gaaggcaatt tcaagatcga aaatgatgaa caggatcgcc accaggtagt agcgaacgtc
     2701 gaacctccta cggctgtctt caaagggttc gaagccgcat tcgtagggcg atactttcgc
     2761 gcggtcgttg cgatagctgg ccgagagccg cccgagcaac gttcccagac ccagcagcac
     2821 cgcggcaagc gccgacgcaa tgcctaggaa gattagcacg ggaaggtagt tcgtgagcat
     2881 tgcaccacca ttgctaaaag gggcggcatg ttgcgcttgc gcgcacattg gaccgccccg
     2941 tggtcgccgt attgtagcag gcgatgctga cttgtggtgc cgacggcgag attcgaactc
     3001 gcacaccttg cggcgctagc ccctcaagct agtgtgtcta ccagttccac cacgtcggca
     3061 ctgtcgtaat tcgcgtttcg ttgtaggccg cgctcacttg ggcgctgtcg gattcgcggg
     3121 cgccgtcggc gttgttgccg gcggcgctga ctgggctgcc ggcgcagagt gcgtcgcagc
     3181 cgcgggcggc gccgattgcg cgggcagtgt gctcgtgggc gcagccgctc cagctgcggg
     3241 tgccggtgcc ggcagcgtca cggtcgctgg cggagcgccc ggggtgcctt tcgctggcgc
     3301 ggccggctgc gcgaattgct ccagcactcc actgctcggc gtcgcagctt tgcgcgtgcc
     3361 cacataggcc agcgacagac tggtgaccat gaagatcgcg gccaaaatgg cggtcgcgcg
     3421 cgacagcgcg gtgctggccc cgcgtgcgcc gaacacggta ccggaggccc ccgcgccaaa
     3481 tccggcaccc gcctcggcgc ctttaccacg ctgcagcagt accagcgcga caatcgaggc
     3541 cgcgcagaaa aaatgaataa tcattaagaa cgtatgcaac atcgatcaac ccgactcaac
     3601 cgcgtgccgc ggccacaatg ctcaagaatt cttctgcttt cagcgaggcg ccaccaatca
     3661 acccgccatc cacgtcggcc tgcgtaaaca gctcacgggc attgcccgcc ttgacgcttc
     3721 cgccatacag gatctgaaga ccggccgcga tcttagcatc ccgctgcgtc agccgggcgc
     3781 ggatgaaccc gtggatctcc tgcacctgcg ccggtgacgc cgtacgcccg gtgccgatcg
     3841 cccagaccgg ctcataggca atcacggcat gcgcaaagct tgagatgccg gtcagcgcga
     3901 ccaccgcctc gagctgccgc cccacgacgt cgaaacagcg cccggcttcg cgctcgggaa
     3961 gctgctcgcc cacgcacagg atcggcacca gccctttcga ctgcgcagcg gcaaacttgc
     4021 gcgccacctg ctcatcgctc tcgtgactga gcgtgcgacg ctccgagtgt ccaacgatca
     4081 cgaactggca accgacgtcc ttcagcatcg cagccgagac ctctccggta taggcaccgt
     4141 gggtctcggc ggagacattc tgcgcaccca gttttaccgc ggtgccgcgc agcaggcgcg
     4201 cgatttcgct caagtagacg tacggcggac acaccacaca ctcgacgctt tcgagcttgg
     4261 gggccgccac aatggcatcg agcagcgcac tgttgtcagt gcgcgagcca tgcatcttcc
     4321 agtttccggc taccagtttg cgtcgcatag tctcgttcgg cctgatttca ggcgcgcgga
     4381 tgatatcggc gacccccttc ctaagcaatg tgccgttctt atcgttgccg gcaggcccgt
     4441 ccgacgtcag gtcggcgccg gggccggcgc cggggctccc cggacgaccg cggccagctc
     4501 ttcggcacag gcgcgcacct cggtcgcatc ccgtgcttcg accatcaccc ggatcaccgg
     4561 ttctgtgcct gaggcgcgca gcacgacccg cccttcgcca tgcagctgct tttcgacccg
     4621 ccgcactgca ttgaccacgt ccggcacact catcgggtca aaccgccgcg ccaccctcac
     4681 attgagcaac acctgcggca gcttctccat tcccgcggcc agctccgcca gcgagcggcc
     4741 ggtctgcttc atgatccgaa ggacctgaag cgccgtgatc agcccgtcac cggtcgtcgt
     4801 gcggtccagt gacagaatgt gtccagaggt ctccccgccg aggataccgc cgctttcctt
     4861 cagcttggcg agcacgtgcc ggtccccgac cggcgcccgc aggaactcaa tgcccagccg
     4921 ccgcagcgcc agttcgagtc caaaattgga catcacggtt ccgaccacgg gcccgcgcag
     4981 acgctgctcg gccttttccg ccgaggcaat cacatacagc agctgatccc cgtccacgag
     5041 gcgtccgagg tgatcgacca tcatgacccg atcgccgtca ccgtcgagcg cgacgcccac
     5101 gtgcgcgtgc acgccgggga cggtcaactg cagcagttca ggtgaggtcg aaccgcagcc
     5161 gtcattgatg tttcggccgt tgggcgaaca tccgatcggc acgatgtcgg catccaggtc
     5221 ggccagaatg cgcggggcaa tcttgtagcc ggcgccgttg gcacagtcga tcacgagctt
     5281 caggcccgag agattgttgc cggcaccgag catgcggttg cagaattcct ggtaatgaaa
     5341 gagcgcgctg tcgacccgcc gtgcgcggcc gatctcgcgc gacgcgcgcg tgagcggcac
     5401 actcaccaac tgcgcttcga tgcgctcttc gatcgcatcg gataatttgc caccctcgcc
     5461 gtcgaagaat ttgatgccat tgtcctcgta cgggttgtga gaggcgctga tgaccacgcc
     5521 gagacggcat tgcatctgcc gggtcatgta ggccaccgcc ggcgtcggta gcggaccggt
     5581 catcagcaca tcggcgccgg ccgcgacgaa ccccgcttcc agggctgctt cgaacatgta
     5641 gcccgagacc cgcgtgtctt tgccgatcaa taccgcgcca ccctcccgga gcagcacttg
     5701 cgctgccgca ctcgccaggc gcaaggcgaa ctcgaccgtc atcggatcag taccgacacg
     5761 acctcgaatg ccatcggttc cgaaataggt tctgctcacg gcggactccc gtagtggatt
     5821 gcgctcgcaa cacgcactgc atcgaccgtc tgggccacgt cgtgcgcgcg cacgatgtgc
     5881 gcaccctgta acactgcggc cgtagccagc gcgaggctcg ccgcgagccg gtcgctgccg
     5941 gcgtcgccct tgcggccaag cagtgtcgcc agcatcgact tgcgcgacaa gccgaccacc
     6001 acgggcaagt gcagcgactc gagctgcggc agtttccgca gcagttgcag attatgctcc
     6061 agccgttttc cgaacccgaa gcctggatcc acgcacagcc gttcgcgagc gatgcccgcg
     6121 tcgacacacg cctgcacgcg atcctgcagg aaggcgcgca cttccttcac gacgtcctga
     6181 tagtgcggat caacctgcat actgtgcggt tcgcccaaca tgtgcattag acacacgccg
     6241 cagggcgtgg aggctgccgc ctcgagggcg cccggccgct gcagggctcg cacatcgttg
     6301 atgagatgag ccccggccgc cggggcccgg ctcatcagtt cgggattcga ggtatcgacc
     6361 gagatcagaa ccttcaggcg cgcggcgata cgctccacca ccggcagcac acgccgcagt
     6421 tcttcttcgc tcgatacccc ccctgctgcc cccggccgcg tcgactcgcc gccgatgtcg
     6481 atgatcgcgg cgccctcgga gctcatacgc tccgcgtgcg cgaaggccgc gtcgggatcg
     6541 aggaatttac cgccgtcgga gaaggagtcc tgcgtgacgt tgaggacgcc catgacgagc
     6601 ggacgagaga ggtccaatgt ccgaccagca cacaccagct gcatctcaag cccccggaaa
     6661 cggcctgtct tcccaggcct tgttggcagg cagatctagt gctggttggc ggcgggccgc
     6721 accactggcg gctcgaccgg acccgcgagc ggagaaggta ccggcttgcc gccgcccgga
     6781 ctcggggtcg aatcatccca gccgtccggc ggacgcggag cacgtccggc catgatgtcc
     6841 ttcagctgat cgtcggccag cgtctcatac ttgatgagcg cttcggccat cgcgtgcagc
     6901 ttctcgatat tggtgtcgag aatctcccgc gcgaccttgt agttgctttc gatcacgcgg
     6961 cgcacttcct cgtcgatcgc atgcgcggtc tcatcggaca cctgcttgtg ctgcgtgacg
     7021 ctgcggccga ggaacacctc gccctgatcc tctgaatagg tgagcggacc caggcggtcg
     7081 gacagacccc acttggtgac catgttgcga gccagttccg tggcgcgctc gatgtcgttc
     7141 gatgcaccgg tcgtgaccga gtccaccccg aagatgatct cttcggcgat acgcccgccg
     7201 aacaacgtcg cgatcgaact ctccagtcgg cgcttgctca tcgagtagcg gtcctgctcg
     7261 ggcaggaatt gcgtgacacc cagcgcacga ccacgcggaa tgatggtcac cttgtagacc
     7321 ggatcgtgct cgggcacggt gataccgacg atcgcgtgac cggcctcgtg ataggcagtc
     7381 atgcgcttct cggcgtcggt catgaccatc gagcgccgct ccgcgcccat catgatcttg
     7441 tccttggcgc gctcgaactg gtccatcgtg accaggcgct ggttggcgcg tgcggcaaac
     7501 agcgccgcct cgttgaccag attggccagg tcagcacccg agaatccggg ggtaccccgt
     7561 gcgatcagcg ccggcttcac atcatccgac agcggaacgc ggcgcatgtg cacgcgtaga
     7621 atctgttcac gtccgcgcac gtcgggcagc ggcaccacga cctggcggtc aaaccggccc
     7681 ggtcgcagca gcgccggatc cagtacatcc ggacggttgg tggcagcgat gacgatgatg
     7741 ccttcgttac cctcgaagcc gtccatctcg accagcaact gattgagtgt ctgctcgcgc
     7801 tcgtcgtgtc cgccgccgag acccgcaccg cggtgacggc caaccgcgtc gatctcatcg
     7861 atgaagatga tgcacggcgc gtgcttcttg gcctgctcga acatgtctcg cacacgcgag
     7921 gcaccgacgc cgacgaacat ttcgacgaag tcggaaccgg aaatggtgaa gaacggcact
     7981 ttggcttcgc cagcgatggc tcgcgccagc agtgtcttgc cggtgccggg agagccgacc
     8041 atcagcacgc ccttcggtat cttgccgccg agcttttgga acttgccggg gtccttcagg
     8101 aagtccacga tttcgccgac ttcctgcttg gcctcatcga caccggcgac gtcggcaaac
     8161 gtgacattga cctggtcctc gcccagcaga cgcgcgcggc tcttgccgaa gctcatggct
     8221 ccacgcccgc cggcggcgcc ctgcatctga cgcaacagat agacgaatac gaccagcaac
     8281 agcatcgccg gagcgagctg caacacgagc tgcgccagga agttcggctg cttgggcggc
     8341 tggccctcga ggctgacccc cgccttgagc aggtcgccga tcagcacgct gtagttcgtt
     8401 tcggggttgt aggtctcgaa gtgagtcttg tctttcaatg tcccgtggat cgtatcgccc
     8461 tgcaacacgg cgctctcaac acgacctgct ttgacgtcgt tatagaactc ggagtagttg
     8521 atcgcctggg cctgggtccc attgggtacg tagcggctga aaaccgccaa cataaccaac
     8581 acgataacga cccagagaag gatgtttttg gtcagatcat tcaacttcgt ttcaccttag
     8641 cgccgacggc ggcctggcgg gagacagact cttgacaggg acgctacacc atcctcttcc
     8701 cggaggccag caagtatgtt tcagaactgc gagatcgcga agcctcaggt ttcagacact
     8761 tgacccgacc aaaactcgta cgagcgctgc ccatcagcgc ctcaaagccg gcaccctgaa
     8821 gcaccttgat cagggcagtg ccgcccggtt tcaaggtttc ccgggccata tccagggcca
     8881 attccgacag gtacatgctc cggggcgcat cgatgacgtc catcccgctc agattgggcg
     8941 ccatatctga cattaacagg tcgatccccg gcgctccgat cgcccgtttg agcgcatcca
     9001 gcacgacctg ttcgcgcacg tccccctgca agaattccac tccggccagg ggctccatct
     9061 ccaatatgtc actcgccagc actcgcccgg gtttaccgac ccgctgtcgc gcatactgac
     9121 tccacgcccc gggggctgcc ccgagatcca gaacaaccat tcccggtttg agcaggcgct
     9181 ccttgcgatc gatctgctcc agcttgaaca ccgcacggga gcgccagccc tcggcccgag
     9241 cccgtttgac gaacggatca gagaaatgtt cgcgcagcca gcgatcgctg ctcttggatc
     9301 ggcgtgccat gttgccttgc ttaagtatca tacgggcgat ggaactctcc gagaaacaat
     9361 tgcgcttcct gcgcggtcgc gcgcatgcgc tcaagcccgt catccaggtc ggccagaagg
     9421 ccctcaccac cggggtcatc gccgaaaccc gccgcgcgct gcaggatcac gaactgatca
     9481 aactgcggat ccaggcgtcc gatcgcgacg ccctcgatgc gctgctggcg gaactggtca
     9541 gcgccgccga agccaccctg gtcgcgcgta tcgggcatgt ggcggtattg ttcaaaccca
     9601 acccgaaact ctcgagaatt cctctgcctg ccaagaacta ggcctgctgc gtggcgccga
     9661 atgaacccgt cagatgtatt tgacggagac gatttcgtac gatcgcgtct tgcccggcgc
     9721 ggcgacatct accacgtcgc cctgcttctt gccgatcagt gcgcgtgcga tcggcgaggt
     9781 caccgaaatg cgaccgccgc ggatgtctgc ctcgtcttca ccgacgatct gataggtgac
     9841 gcccgttcct tcggcctgat cctcgagctc cactgtcgca ccgaacacga cccgaccggt
     9901 cgccggcaga ctggccacgt cgatgatctc ggccgctccc aggcgcgact cgatttcacc
     9961 gatccggccc tcgatgaatc cctgctgctc acgagccgcg tgatactcgg cgttctccga
    10021 tagatcgccg tgactgcgcg cctcggcaat cgccttgatg acgcgcgggc ggtcttcgga
    10081 tttcaggcgc ttgagctcgg tgcgcagcag ctcggcgccc cgcagcgtca tcggtgagcg
    10141 tttcatgtgc ccacctcgcg acgcacctcg cgcagcgtcg tagtcgcgcc ggtactgccc
    10201 tgactggcag gcatgatggc cacttccttg tgcagatcct gcagacgatt cacgtccact
    10261 tcgtcgatgt gatcgagcgc ctcgcaggtc gccagtgccg ctgccatggt ggtgtagtag
    10321 gtcacacgcc gatgcactgc ttcgcggcga atcgagttcg actccaggat cgcctgcttg
    10381 ccttccgtgg tgttgacgat cagatcgatc tcatcgttct tgatcatgtc tacgatatgc
    10441 ggccggcctt cgcgcacctt gttggcggtg cggcactcga cgcccgcggc cttgagcgct
    10501 gcggcggtgc cccgggtcgc gaccagcgtg aacccatgtg ccatcaggcg gcgggcgaga
    10561 cccactgctg cggccttgtc tcgctcgcgc acgctgagca gcgccagccc cgagcgcggc
    10621 aacaccacgc cggaagccgc ctgggctttc gcatacgcct cgccgaatgt gcggcctgtg
    10681 cccatgacct caccggtcga tttcatttca ggtccaagga tcggatcggc ttccggaaat
    10741 ttgatgaacg ggaataccgc ttccttgacc gaatagtagc gcggcacccg ctcctcgagc
    10801 acgcccattt cccgcagctt gcgtccggtc atgacccgcg cggcaatctt cgcaatcggc
    10861 acaccggtcg ccttcgacac gaatggcacc gtgcgggaag ctcgcgggtt gacctcgagc
    10921 aggtagacgg tgccgttctg aatcgcgaac tggatgttca tcagcccgac gacgttcagc
    10981 gcgcgggcga gcttgcgcgt ctgctggcgc atctcattct gtaactctac tgaaaggctg
    11041 ttcggcggca gcgagcagcc ggaatcgccc gaatgcaccc ccgcctgctc gacgtgctcc
    11101 atgatgccgc cgatcagtac gtcctgacca tcgcagaccg catcgacatc gacttcgatc
    11161 gccacgtcca ggaatcggtc gagcagcacg ggcgcatcgt tcgacacctg caccgcacgc
    11221 gtcatgtagt tgcgcaggtc atcctcgttg aagacgattt ccatcgcccg gccgcccaga
    11281 acataagagg gccgcaccac tagtggataa cccacctcgc gcgccagcat catcgcctgc
    11341 tcttccttac gggcgctggc attggccggc tgcttgagct gcaggcgacg caccagttgc
    11401 tggaagcgtt cgcgatcctc ggcgacgtcg atcgagtccg gtgaggtgcc gatgatcgca
    11461 gcgcccgcct cttccagtgc gcgggacagc ttcagagggg tttgaccacc gaactgcacg
    11521 atcacaccct cgggccgctc cttcgcgatg acctccagca cgtcctccag ggtcagcggc
    11581 tcaaaataca gccggtccga cgtgtcgtag tcggtcgaga ccgtctccgg attgcagttg
    11641 accatgatgg tttcaaaccc atcctcgcgc agcgcgagcg ccgcgtgcac gcagcagtaa
    11701 tcgaactcga tgccctggcc gatgcggttc ggaccgccgc ccaggatcat cactttgcgc
    11761 tgtttggtcg gctcggcctc gcactcctct tcataggtcg agtacaggta cgcggtggag
    11821 gtggagaact ccgcggcgca cgtgtccact cgcttgtaga ccggcgcgat gccgaattcg
    11881 cggcgcttct tgcggatcgc gccctcgttg ctgcgggtca gcgtcgccag ccggcggtca
    11941 gagaagccct tgcgcttgag cgagcgcaga cgctcgatac ccagcgccga ctcaccctca
    12001 cgacgggcgc gggtctcttc ggcgatcaga tcggcgacct gcgccagaaa ccagggatcg
    12061 atcgcgctca gctccgcgac gcgcgccacc gtccacccgg cgcggaacgc gtcggccaca
    12121 tacagcagtc ggttcggccc tggcgcccgc aactcatagt tgagctgccg ctcggtttcc
    12181 tcggtcagcg gcagggtcag catcggatcg agcccatcca gcccggtctc caggccgcgc
    12241 aaagctttgt gcagcgattc ctggaaggtg cgaccgatcg ccatcacctc gccgaccgac
    12301 ttcatctgcg tggtcaggcg cgtattggcg gccggaaact tctcgaaggt aaagcgcgga
    12361 atcttggtga cgacgtaatc aatcgtcggc tcgaacgagc tcggtgtcgc gccaccggta
    12421 atatcgttgc gaagctcatc gagtgtgtag ccaaccgcca gcttggcggc gaccttggcg
    12481 atcggaaagc ccgtggcttt cgaggccagc gccgaggagc gcgacacgcg cgggttcatc
    12541 tcgatcacca gcatgcgacc ggtgaccgga tcgatcgcga actgcacgtt ggaaccgccg
    12601 cactcaacgc caatcttgcg cagcaccgcg atcgaggcgt cgcgcatgcg ctgatattcc
    12661 ttgtcggtca gcgtctgcgc cggcgccacc gtgatcgagt caccggtgtg cacgcccatc
    12721 ggatcgacgt tctcgatcga gcaaacgatg atgcagttgt cgttgcgatc gcgcaccact
    12781 tccatttcga actctttcca gccgagcacc gcctcttcca gcagcagctc acgcgtgggc
    12841 gaggcatcca gcccgcgctg cacgatggtc tcgaattcct cgcggttgta cgcgatacca
    12901 ccgccactgc ccccgagtgt gaaggacggc cggatcacga tcggatagcc gatgctgctc
    12961 tgaaccgcca gcgcctcctc gaggctgcga gcgatctgcg aggtgggtac ctccagtccg
    13021 atctcgcgca tcgcgttgcg gaagcgctcc cgatcctccg ccatgtcaat cgcttcgcgc
    13081 gaagcggcaa tcatctcgac gccgaattgc tgcagcaccc cttcgcgtac cagatcgagc
    13141 gcggtgttca gcgcggtctg gccacccatg gtcggcagca acgcgctcgg gcgttcgcgc
    13201 tcgatgatcc gggccaccgt gcgccagttg atgggttcga tgtagatcga atcggccgtc
    13261 tccgggtcgg tcatgatcgt ggccggattg gagttcacca gaatgacccg gtaaccctct
    13321 tcgcgcagcg ccttgcacgc ctgcgcgccc gagtagtcga actcgcacgc ctgaccgatc
    13381 acgatcgggc cggcgccaat gatcaggatg ctattgagat ctgtgcgttt gggcatggct
    13441 tgaccgccct agccttccgc gctctgtttg cgcgcattgg cacgcagctg cttcagctgg
    13501 ttctgcaatc gtcgcaccat cagctgaatg aagttgtcga acagcggttt caggtcgtgc
    13561 ggaccggggc tcgcctccgg atgcccctga aagctgaagg ccgccacatc gctgcgggcc
    13621 agaccctgca gactgccgtc gaacagcgag cggtgcgtgg ccaccagctt gtcgccgagc
    13681 gtggattcat cgaccgcgaa gccgtgattc tggctggtga tcatgactcg gccggttgag
    13741 agttcctgca ccggatgatt cgcgccgtga tggccgaatt tcatcttgac ggttttagcg
    13801 ccgctcgcaa gtcccagcaa ctgatgcccg agacaaatgc cgaacaccgg tacgtcggtc
    13861 gcgagcagtt cgctgatcgc cttgatcgcg taatcgcaag gctccggatc acccggaccg
    13921 ttggacagga atacgccgtc cggattgacc gacagcacgt cctgcgcgga cgtggtcgcc
    13981 ggcacgacgg tgatgcggca gccctgatca gaaagcaccc tcaggatatt gcgcttgacg
    14041 ccgtagtcgt aggccaccac gtgcagacgt tgttgcgcgc gtagcagatt cagcggctcg
    14101 cgccccaccg agccttcatt ccattgatac ggctcacgcg tggtgacgac cttcgccagg
    14161 tccatcccct tcaggcccgg gaaggcgcgc gcggcacgta ccgcagcgtc ttcgttgatc
    14221 cgatcgccgg tcatgatgca gccggactgc gcacctttct cgcgcagcag gcgcgtcagg
    14281 cgacgcgtat cgatgccggc aatcgcgacg atcttgccgc gctgtaggaa ctcacccagc
    14341 gactccgccg cgcgccagct actggggatc atcgacaggt cgcgcaccac cagaccggag
    14401 gcgaagatct gcgaggactc catgtcctca ggcgtcacgc cgtagttgcc gatgtgcgga
    14461 taggtcagtg tgacgatctg gcgcgcgtac gaaggatcgg tgaggatttc ctggtagccg
    14521 gtgatggcgg tattgaaaac gacttcgcca gtggtgtttc cggaagcgcc aatggattga
    14581 cctttgaaga tcgaaccgtc gcccagagcc agcaccgctg gtgcagtcac gtcatccctc
    14641 tccttcaatc cccaaggtgc agccaaatcg cgcgtgccat gagattttca gcatgcacat
    14701 gcacaaagcg ggaggagttc ttttggaaca tcctcccgcc ttgtgcggac taacccgata
    14761 ctcggattac ggggcggagt ttagagaccc cacccgcccc tgtccacgcc aatttccgcc
    14821 gaaattttgt ccgcatttgc gtcgcgcccg actatccaaa cgaattcggt cgttagcagg
    14881 cagatataca gaaatagcct tagctaatat acatatttac ttgaatttac ttatgaatac
    14941 atccctcatt gcataacgac cagccatcct cccggccagc cactgtcccg ccaacagcgc
    15001 tccgcgagcg aacaccgcgc gatcggtggc cgagtgcttc agaagtaccc gctcgccctc
    15061 gcccagaaac catacctcgt gctcacccac cacatctccg ccgcgcacgc tgacaaagcc
    15121 gatctcatcc tcctgcctcg gttcaccgat cccatggcgc gcatagaccg cctgcctgga
    15181 aagactcacc gcccgcccgt ccgctgcggc cgcccccagc gccagggcgg tccccgatgg
    15241 tgcatcgcgc ttggctcgat ggtgggtctc gacgatctcg atgtcatatc cgggtggcag
    15301 tccctgtgcg gcttgccgca ccagttccat cagcaggttc acgccaagac tggtgttcgg
    15361 ggccaccagc aacgcgatcg aatcggcggc cgctgccagc ggcgcgtcca gttcgcgcgg
    15421 caatccggtc gtgccaatca gcagcgctac ccgcgccgcg acgcaggcgg ccagattcgc
    15481 tgccgcggcg ctcgggtggg aaaagtccag aatcagctcc gcctcgcgca gcagcggtgg
    15541 cagcgaggcg ctgatcgcga tgccgcaagg ctcgacgcct gcgtagcgac ccgcgtcctg
    15601 acccagaaac tggctgtcct cggtggccac cgcgccgtgc aggcgcagcc ccggaaactg
    15661 tccgatcagc cgcagcagtt gtccacccat gcggccggtc gcaccgacga tgacggtgcg
    15721 tcgtgcgccc gcggcggcgg ccgtcatgtg gaatcccgcg agaagaagcg cttcacacca
    15781 tccagaaaac tcttctgacg gggcgcgtgc ttgctgccat cgtgcttcac ggagtcatcg
    15841 aactgcttga gcagtgcccg ctgctcggca gacaggttca cgggggtttc gaccacgcag
    15901 cgacagaaca gatcaccccg ggcaccgccg cgcactgatt tcacaccctt ttcacgcagc
    15961 cggaaaacgc gccccgactg gctctctgca ggaacggtca gcgccacctg cccgtccagc
    16021 gtcggcacct cgaccttgcc gccgagtacc gccgttgcaa atgaaatcgg cacttcgcag
    16081 ctcaggtgca caccgtcgcg ctcgaagatc ggatgctcgc gcacgtgcac ttcgacatac
    16141 agatcccccg gtgggccgcc gttgcggccc gcctcgccct cgccccctag gcgcacgcga
    16201 tcgcccgtat ccacaccggc cggaatcttg accgagagtt tcttggcccg acggacccgt
    16261 ccctggccca gacaggcatc acacggattt cgcacgatac ggccgcttcc gcggcagtgc
    16321 ggacaggttt gctgcagcgt gaaaaaaccc tgcgagaccc gcacctggcc gttgccgccg
    16381 caggtgtcgc aggtgacagg attgctaccc ttggcagcgc cgctgccgcc acaggtgtcg
    16441 cactccacca gtttcttgac gtcgatctcg agcgtatggc cgaacacggc ctcggacaga
    16501 tcaagttcgg cttcgtagcg cagatccgcg ccgcgaaaca cctgtgaggc gccgccgcgc
    16561 cgtccgccac cgaagatatc gccaaacaca tcgccgaaga tgtcgccgaa gccttcggcg
    16621 ccctgaaatc cgccgccacc tgccgatgcg ttgcggctcg cctcgatgcc ggcgtgaccg
    16681 tgctgatcgt agatggagcg cttctgcgca tccgacagga tttcgtaggc ttccttggct
    16741 tccttgaatt tctcttcggc ttccttgtct ccgggattgc gatccgggtg gtatttcatc
    16801 gccagacgac ggtaggcctt cttgatgtct gcctcggtgg agtttcgggc cagatcgaga
    16861 gtcttgtagt agtcgcgctt cgtcgccatg tctgcctgtc gtgccattgt tctcaagcgt
    16921 ccgtcacgtg caccaaggcc gccgggcacc ttgcggcgcc cagcggccct gacgactcaa
    16981 gtcactcgcg ctcgcgcgcg aatgttgtca ggaagcgcga cggcccttgt cgcgcacttc
    17041 ctcaaactcc gcatcgacga cgttttcgtc cgccttctgc tgcgagcctc cggcactgcc
    17101 agaagctccc gctccagcgg cggcggaggc ctcgggaccc gcggcgcccc ccggaggatc
    17161 gccagcgccc gcgccgccct gattggctgt gtaggcacgc tgcgcgatac cggctgaagc
    17221 ctggcccaac gcctcggtct ttttctcgat cttctccttg tcgtcggact tcaggaccgc
    17281 cttcagatcg ttcagtgccg cctcggtctt cccacgctca tcggccgtga ccttgtcgcc
    17341 cagatccttc aacgctttct cgaccgcatg gatcatcgca tcggcgcgat tgcgcgtttc
    17401 gatcagctcc cggaacgcct tgtcctcggc cgcatgcgcc tccgcgtcct tcaccatgcg
    17461 cttgatctca tcctccgcca gaccgctgga ggcacggatc acgatgcgct gctccttgcc
    17521 ggtggccttg tccttcgctg acacattcag aatgccgttg gcatcgatgt cgaacgtgac
    17581 ttcgacctgc ggcacgccgc gtggcgccgg gggaatatcg ctcaggtcaa acttgccgag
    17641 tgacttgttg tcgcccgacc gctcgcgctc accctgcagt acgtggatcg tcaccgcggt
    17701 ctgatggtct tccgccgtgg agaacgtatt ggctgccttg gtgggaatgg tcgtgttctt
    17761 ttctatcagc ttggtcatca caccgcccag ggtctcgata ccgagcgaca gcggcgtcac
    17821 gtcgagcagc aacacgtcct tgacctcacc ggccagcacg ccggcctgca ccgcagcgcc
    17881 caccgccacg gcctcatccg gattcacgtc cttacgcggc tccttgccaa agaagtcctt
    17941 cacatacttc tgcaccagcg gcatacgggt ctgaccgccg accagaatga cttccgccac
    18001 atccgaggcc gcaagccccg cgtccttgag cgcggtccgg cacggtccga gggttcgctg
    18061 cagcaggtcc tcgaccagcg actcgagctt ggcccgtgtg agcttgacgt tcagatgctt
    18121 cggacccgag gcatcggccg tgatgtaggg cagattgacg tcggtctgct gactggagga
    18181 cagctcgatc ttggccttct cagcggcttc cttcaggcgc tgcatggcca gcggatcacg
    18241 tcgcacgtcc acgccggatt ccttcaggaa ttcatcggcc aggaaattga tgatgcgcag
    18301 atcgaaatcc tcgccgccga ggaaggtatc gccattggtc gccaacacct cgaactggcg
    18361 ctcgccatcg accgccgcga tctcgatgat cgagacgtcg aacgtgccgc cacccaggtc
    18421 gtacaccgcg atcttgcggt ccgcgccctg cttgtcgagg ccataggcca gtgccgcggc
    18481 cgtcggctcg ttgatgatgc gctgcacgtt gaggcccgcg attcggccgg cgtctttggt
    18541 cgcctggcgc tgtgagtcat tgaaataggc aggcaccgtg atcactgcct cggtgaccgg
    18601 ctcgccgagg taatcctcag cggtcttttt catcttcatg agcacgcgcg cggaaatctc
    18661 cggcggtgcc atcttcttgc cccggatctc gacccaggcg tcaccgttgt ccgccttgac
    18721 gatcttgtag ggcaccatct tgatgtcctt ctgcacgacc tcgtcctcga acttgcgtcc
    18781 gatgagtcgc ttgaccgcga aggtggtgtt ctgcggattg gtgaccgcct ggcgcttggc
    18841 cgactggccc accaggactt cgtcgtcctt ggtaaaggcg acgatcgagg gtgtggtccg
    18901 atcgccctcg ctgttttcga tcacgcgcgg cttgccgcct tccatgatcg cgacgcacga
    18961 gttcgtcgtg cctagatcga tgccgattac cttactcata agcgcactcc agcaatgtca
    19021 ttttcatatg ccacaacaaa ctggggccca ggccccgatt ttcaactgta aacggactgg
    19081 ccgcgaccac atcaaccatc gttcgtcttc gcgacgatga cacgcgcggg ccgcagcagc
    19141 cgtccattga gttcatatcc ggtctgcact acctgcagca cgcggtccgg ttcagaagtc
    19201 tccgacggtt gcatcagcac cgcctcgtgc tgagcaggat tgaatttgtc ccccagtgga
    19261 tcgaggattt tgacgcgggc tttgtccagc gcccggtcca gcagtttcag cgtcgcctgc
    19321 tgtccttcga gcagggtctt ggcatcggcc tgtgcgccct ggcgcgcggc cagttccaat
    19381 ccatcgcgca ccggcagcag ctcgccgacg aagcgctcga gcgcgtagcg atgcgcgctc
    19441 tcgatatcgc gctcggcgcg cttgcgcacg ttgtccagtt cggccagcgc cctcaaatac
    19501 tgatcgcgct ggctgcgcgt tgcgtcctgg gccgcggcca gggccgtgcg caaagattgc
    19561 agttccgcgg cagcaccgga ggcgagcggt tcttcgctgc cctcaagctg cggctcaggc
    19621 tgatccatcg caggcccgtt ttgcgttgcc tctgtcacta aattaccctt taaactcaat
    19681 tagctacaaa ctattattaa aacggattct cgccgcagct ccgcccgtca acgcaaacta
    19741 tggcgcctaa cgacgtgaat tcaaggccga accgagaact tttgccgtca gatcgacgat
    19801 cggaatcacg cgttcatagg ccatgcgagt cggaccgatg accccgagca caccgaccac
    19861 acccttggcg gagctgtacg gcgcggtcac gacactgtac gcatccagga tctgatagcc
    19921 ggattcctgg ccgataaaga tctgtacgcc ctcggcgcgc aggctttgat cgagcagatg
    19981 caggatatcg cgcttttcgt tgaaggcctc gaacaggcgc ttgagccgat ccacatccga
    20041 cacgccgctg aagcccatca ggttggtctc accggcgatc acgtattcga tgtcttcact
    20101 gggggcggca ccctgttcga acaggcgctg cgacatcgca atcgcaccga gcatgctctg
    20161 attgagatgt tcgtgcgttt ccttgagctg gcgcaccagt tcctgatgga tctgcgcggg
    20221 cggcttaccg acgcaatgct cgttgaggaa attggccgcc cggcgcagtt cttccgaaga
    20281 atagtggcgc tccagctgca cgatgcggtt ctgcacttcc tggtcatcca gcaccagcac
    20341 caccaggacg cgattctccg acagccccag gaactcgatc tgcgtcagca ccgccgagtg
    20401 tgccagcggc atggtcacga cccccgcgag atgcgtgatg ttggacagca gctgcgaggc
    20461 ggcggccacc agtgccttgg agctgtcctg tggcatgtcg agctggcgtc ggatttcctc
    20521 caggctcgca tcatcggtcg gcatctgcgc ccgcagcagt gaatcgacga agaaccggta
    20581 ggccttgtcc gtcggcaccc ggccggcgga ggtgtgcggg gaggtcacga atccgagccc
    20641 ttccagatcg gacatcacat tgcggacggt ggcagaactc aaggtcagcc cggattcgcg
    20701 cgacagggtg cgcgagccga ccggctgccc atcgcggatg tagttctgga ccagcacccg
    20761 cagcagccgc tgcgcgcgct cgttcagagc atcatcccga ctatcttgtg acatgcttga
    20821 ttcgcagagc taaccagcgg tcgccgagcc cgtcaagaca ggccgggatc gaacccagtc
    20881 ctcagatccc ggtatttctc tcaatatgga aagtcctacg cgttggcgcc tacaggccgc
    20941 catgctacaa cagcattgga caataaggcc ctcaagccga aagatgcaga agccaatcaa
    21001 gacttgcgcg ctggtgggca gatttcagga cgcgcgcgtg gctgaatgtg cggtgcccct
    21061 gatttcggtg ctgcagtcgc ggcaactgaa ggtgctggca ctacaggatt cgaccgcccc
    21121 gtcggccccc gccggggtgg cgatcgtgcc gctcgcccaa ttgatcgagc aggcggagct
    21181 gatcattgcg attggaggag acgggaccct actctacgcg gctgggctgg tcgccgagca
    21241 cgatataccg ctgctaggca tcaaccgtgg acgactgggc ttcctcacag atgtgctacc
    21301 gcaggacatg gaaggctgca tcggagcggt gctcgcgggc gaatgcgagg cggactcccg
    21361 tcccctgttg caggccaggc ttcgagggtc cgatggcagc gaagtgcagc gcttcgccct
    21421 caacgacgtg gtcttcaatc gtctggagac cggtcgcatg ctcgatttcg aaacccgcat
    21481 cgacgggcgc tacgtcaatt cgcacggcgg tgacggcctg gtggtcgcca cggctaccgg
    21541 ctcgaccgct tatgcgctct cctgcggcgg accgatcgtg aatccgaagc tgaacgtgct
    21601 ggtggtcgca ccgatctgtc cgcacacgct ctcggaccgg ccgatcgtgg ttcccggtga
    21661 gtcgcgggtg gatatccggc tgatcggaga gacaccgacc accgcgcagg tcacttgcga
    21721 tgcggcggtg ctgggcgagc tgaaaaccgg cggtcggctg gagatctttc cagcgggccg
    21781 ttcggtcacc ctgctgcacc cacccggcta tgactacttc cggttgcttc gatccaaact
    21841 tcattggggc ctgggcggcc tgactcacga gcgctgaaga atcgtgttac gccatctgca
    21901 aatccgcgac ttcgcgatca tcgaatcggt cgaactggac ttcagccccg gcctgacggt
    21961 gctcaccggt gaaaccggcg ccggcaagtc catcctggtc gatgcgctgg aactgctctc
    22021 cggcggccgg gcggaaaagg atgtgatccg cgccggtgca gacaaggccg atatcacagc
    22081 aacgattgat atttcgaaaa tcgcaggtga gctgcgccat attctggaac agcaatcgat
    22141 caccagtgac gtggatctga tggtgcgccg ggtcgtcagc agcgacgggc gcacccgcgc
    22201 ctggctgaat ggccagtcgg tgccgttgca gctggtgcgc agcgtgaccg aactgctgtt
    22261 cgatattcac ggtcagcacg agttccagtc gcttgtgcgg ccggcgactc agcgcgcact
    22321 ggtggacacc tacggcaagc tcgaagctct cagcggacag gtgcgggcgg cacacagcac
    22381 ctggctggca ctgctcaatc gcagcgtcga gctggaagcc gccgccagcg accgcggctc
    22441 gcggctggag ctgctgcgac atcaggtccg ggagattgag gcgctgcaga tcaagccggg
    22501 tgagaccgcg caactgaagg aagagcgctc gcgggtggcc aacagcagcc gactgatcga
    22561 aggggtgcgc gcctcattgg aatgcctgtt tgaatccgac caagccagcg cccacgccct
    22621 gctctcgaag tcactgtcat cgctgcggca ggccagccac ctggacccgc agctcgaccc
    22681 attcgcggcg ctgctggacg aggcgctgat ccgcgtcacc gatgcgagcc actcgctgca
    22741 gcagtacctc gacgcgctgg acctcgatcc acagcgcggc gacgcgatcg agcgccggct
    22801 ggcggccttc gaggagctgg cgcgcaaaca ccgcatctcg cccgacgcgc tgctggagaa
    22861 acagtcggag ctgacattgc agctgggcgg actggaacac gccgccgccg acctgacgac
    22921 ggtgcgcacc caactggccg ctgccggaaa cagctaccac gaaatcgccc ggcaactctc
    22981 tgcacgacgt acgaccgccg cgcgcgggct ggccaaagag atctcggcgc gcatgcagga
    23041 gctcggcatg gccggtggcc gcttcctgat cgatgtcacc cagaacgagc acgccgagcc
    23101 caccgccaat gggctcgatc agatcgagtt tcgggtaagc accaatccgg gccagccacc
    23161 gcgggcgatc gccaaagtcg cctccggcgg cgagctggcg cgcctgtcat tggcagtaca
    23221 ggtctcctgc gcggagagtg gcgccggctg catggtgttc gacgaagtgg atgccggcat
    23281 cggtggcgca gtggcggaga tcgtcggccg tgaactgcgc gcactggggg aacgcggcca
    23341 ggtactgtgc gtcacgcatc tgccacaggt ggccgcacag ggtcatcagc atctgcgtgt
    23401 cgccaagaag agcgagggca agctcacccg cacgaccgtc acgccgttga gcgcggaagc
    23461 gagcgttgac gaaattgcgc gcatgctggg cggggtggaa atcaccgcac gggcccgggc
    23521 tcacgcgcag gaaatgctgt cgaaggtgcc caccagcaaa cgcaagagcg gctagctcgg
    23581 tacctttact cgtccctgtt tcttggccgg acagtcgacg cgctgacagc ggccatacag
    23641 caccatcgaa tgttcatgga tgcgatagcc aaggcgcgtt gcgatcgaat tctgcttggc
    23701 ctcgatctcc ggatccacga actcttccac ggcgccacag tccatacaca cgatgtgatc
    23761 atggtgagtg ccgtggttga tctcgaacac cgccattccg tcctcgaaat ggtggcgcgc
    23821 gaccaggccg gcgctctcga actgagtgag cacccgatag accgtcgcca atccaatgtc
    23881 ttcactggag tcgagcagct tcttgtagat ctcttcggcc gacatgtgtc gcgtagcgct
    23941 gtgctcaaga atctcaagga tcttgagccg cggcagagtg accttaagac cggcccgacg
    24001 cagatctttg ccttccacgc tacacctcac ggtatgatga gtggcatgag aattatccca
    24061 ctatttgcac tgctcggggc cctcagtggc tgcgtctatc gcatgcccat ccagcagggc
    24121 aactatctgg acccggcagc gattgcccaa gtcaaagtcg gcatgacgca cagccaggtg
    24181 cgcttcctgc tgggcactcc catggtgccg ggtgcctttg acgacgcgcg ctgggactac
    24241 gactactatc tcaagatcag acgactgcgt ggccccgcga ccggacacgc gacggtgtac
    24301 ttccagaaca atctggtcac acgcgtcatc agcgacgtca aggaacctcc gatgctgccg
    24361 ggcctgggcc ggccgatcgc gccccgcccc aattagcacc gccgctgcct gtcgccagca
    24421 cgcttagcgt ttgtcgccgc gatggggcaa ggcccgctcg cgtcgcttgg cacgcgggtc
    24481 cagctgcagc ggccgataca gttcaatccg atcgccatcg gcgaacacat ggctgcgtac
    24541 gcgcagccta ccgtagatac cgattgcagt gtgttcccag tccgtggcgc cctcgcccag
    24601 ctggtcacgc gctgccgcca gcgcgcgatc gatcgtggcg tcatccggca actccagctc
    24661 acacgccagc agccctgccg cggtatcgca cacgacgctg cagtgcttca agccggcatg
    24721 ctctgcgcgc ggatgacgaa ggcccgcacc aacgaggcgg cggtctcttc gaacagcggc
    24781 tcgaacagcg ccgatttgag caggttcgag aactgaaagc gcagcgagag ctcgatgcgg
    24841 cagccattgc tggcgaccgg tgtgaaactc catttcccct cgagcatccg aaacgggccg
    24901 tccaccagat ccatctgcat gctgcgcccg ggttccaggc gattgcgggt cgtgaattcg
    24961 gtgcgcagcg gaccgcgccg gacagccagt cgcgcaacca tttcagtcgc gctgcgttcc
    25021 agtactttcg cctcggtgca acccggaacg aactgcggat accgctccac gtcttcgact
    25081 acctgataga ccagttccgg cgagcgtgcc acgagggccg aacgcgtcat ttctcgcatc
    25141 gatccagttt ggcttaagct tgtcgcaccg acaacatcaa atacgcgtcg ggcggcccag
    25201 ccgctgcccg attcaagagg aatccaatgc ccaccaagac ggcgagtctg gcgctgaacc
    25261 ttgctttcat tgttgctgtt tccgcagcgg gtgcggcctc cgacaacacc tcctcgagcg
    25321 catcgatggc acccccgatc tcgtcgagca gatcgtgaag gcacacagct gatggccaag
    25381 gcggaaagcc cgcagcgcgt gattgccgag aaccggcgcg cacgcttcga ttacttcatc
    25441 gaggagcgct tcgaagccgg actcgcgctg gagggctggg aggtcaagtc gatgcgtgcg
    25501 ggtcgcgcgc agctggccga agcctacgtc tatgtgcgca acggcgaagc attcctgatc
    25561 ggtgcgcacc tgtcgccgct gcgcaccacc tcgacccaca aga
//
LOCUS       DATMRM010000156         5488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4117105, whole genome shotgun sequence.
ACCESSION   DATMRM010000156 DATMRM010000000
VERSION     DATMRM010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4117105"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1995)
                     /locus_tag="VNZ06_04690"
     CDS             complement(<1..1995)
                     /locus_tag="VNZ06_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWW20080.1"
                     /translation="MSPAPSQAQTTAGTGAAPTEAVLTEVTITAEKKSENLQTTSVSA
                     TVRNGEELKEEGVLNIQDLQLETPGLTIQPPLSSETFINIRGVGIQQTNPASSNGVAF
                     YVDGQAIPSLIDTVDTFYDIQDVEVLRGPQGTLVGSNSDGGAIFVNSVQPSFDALKGY
                     LEQTFGNYNDRRTEGAVNLPLSDMFAARVAFVYETRDSFTTNLGAQASSPIAAAAGFP
                     GAQNQPGNINFNAVRAQLAFRPNEDFEYTLRYEPYSSRTDGFAVKPDEAAIGPLGAYF
                     KVPTTRFADPTAAAIQNQAFTIDYDRNQYYNISGQRSTGTGIWHMTPDVELRSVSSYQ
                     SGYESDLTDVDASSAPADVYLVRRADFRTFTQEFNLLSTGPGPLQWVVGAYYLNSRGP
                     LSLQFVNGGGGPLSVISYHTNVAGFASATYQFTDQWSFTLGGRYSHDNQPFHELGHLP
                     IPNEVFKTTASEPTGTAKINFQATADTLIYASVSTGYKAGGVNLQIPPIITPPPFKPE
                     KNVVEELGLKTTTLNGHLRFDGDVFNSHYTDFQVQETIVANPAPGVFIPAPYTQNAGS
                     ARIWGAEAEFTGAFDQLQANFGASYLNAKTSGTFTFTGPTGIPYQIPSGTDVPYAPDW
                     TITAGVQYGLPLASGVLTPRLQYQYQASQFIGISHNIFPLN"
     gene            2264..3973
                     /locus_tag="VNZ06_04695"
     CDS             2264..3973
                     /locus_tag="VNZ06_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HWW20081.1"
                     /translation="MAKFDDLFYHSATGVSMINRRILTQGILGAGVAALTTGHRIVAQ
                     EQRQGEAVAKTTLGSIRGASQDGILSFLGVPYGAAPTGDLRFMPPRSAQPWTDVRDAR
                     AYGDTCPQIPLGLSPFVRHGDGPPPPPSPMQRQLGTLFTRGAKEPAQSEDCLVLNVWT
                     RGLEHGRRPVMVWLHGGGFAVGSGSLPTYNGARLAARGDVVIVTINHRLNVFGHLYLG
                     EIAGERFAQSANVGMLDILAALQWVRDNIAGFGGDPGNVTIFGESGGAGKVSVMCAMP
                     SARGLFHKAIMQSGPCLQIIEKARGTAIARQLLQDLGLSPNQVVELQHMDAMKLAGAA
                     EAAEVKVVPRILGFGPTGLIPLVDGVVLPHHPFDKVASPESAHVPFLVGSTKDEAVLF
                     TGPLPRWGQFTDDELLALLGPIAGARSREALTLYRRLHPADSNSYLLADMVTDFWMRQ
                     AANHVAELKAQQHSAPAYQYVLEWEINQDLRTAHGTDVSLVFDNVAASAAVSGAPNAQ
                     AVSDQMSDAWLAFAKTGNPNHARLAHWPAYSLPARAMMLFNVNSHVIDDYEAPARAFW
                     EAR"
     gene            4050..5066
                     /locus_tag="VNZ06_04700"
     CDS             4050..5066
                     /locus_tag="VNZ06_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20082.1"
                     /translation="MRSTLAVTTVAILSLLLAPTLPAQAPGAPGNPGTASLLPPPDAP
                     PIMPAPPGPYPITIEANAALPTHTLYRPADLTPFNGPKRLPIIAWGNGACSNAGLLFK
                     EFLSNIASYGFMIIVSGPKDAPLPAFARAAQRAASSSGSAAPPPTPPAPGTPRPPMTT
                     DADMQTAIDWAIRENGTARSPYHAKLDPQKIAVMGQSCGGLQAIANSTDPRVKTSIIW
                     NSGLFPDGQIGHSLSGATKATLAKIHAPIAYINGGPADAAYVNTLDDIQHIEGVPLFF
                     GWINVGHGGTYTHPGGGRFAEVAVAWLNWRLNGDKQAAKMFDGPDCTLCKDPIWHVQK
                     KNMN"
     gene            complement(5083..>5488)
                     /locus_tag="VNZ06_04705"
     CDS             complement(5083..>5488)
                     /locus_tag="VNZ06_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20083.1"
                     /translation="GLLEGRQRTPSILVTFVMVPILLVALARSPAQLSIVVSVVCLLG
                     CAVLAAQAFLYAMAPTLYPTSIRGVGVGAAVAIGRIGSIVGPKLGGMLRAAGHGYSQL
                     LMDLLPIAIIASLCALVLAWKIPSRGLNGHSS"
BASE COUNT         1023 a         1728 c         1734 g         1003 t
ORIGIN      
        1 attcaacggg aaaatgttat gcgagatccc gatgaactga gaggcctggt actgatactg
       61 cagtcgcggc gtcagcacac cgctcgccaa cggcaaaccg tactgcacgc cggcggtgat
      121 ggtccaatcc ggcgcgtacg gcacgtccgt gccggatgga atctggtacg ggattcccgt
      181 cggtccggtg aaggtgaagg taccggaggt cttggcgttc agataggacg caccgaagtt
      241 cgcctgcagc tgatcgaacg ccccggtgaa ctccgcctcg gcgccccaga tgcgcgcact
      301 gcccgcgttc tgagtgtacg gcgcgggaat gaatacaccc ggcgcggggt tggcaacgat
      361 ggtctcctgc acctggaaat ccgtgtaatg cgagttgaag acatcgccat cgaagcgcag
      421 atgtccgttg agcgtcgtgg tcttcaggcc aagttcctcg acgacgttct tctccggttt
      481 gaacggcggc ggggtgatga tcggcgggat ctgcagattc acgccaccgg ccttgtagcc
      541 ggtcgagacc gatgcataga tcagcgtgtc ggcagtggcc tggaagttga tctttgccgt
      601 tccggtcggc tccgaggcgg tggtcttgaa cacctcattg ggaatcggta ggtgtccgag
      661 ctcgtgaaag ggctggttgt cgtgcgaata gcgcccgccc agtgtgaagg accactggtc
      721 agtgaactga taggttgccg aggcgaaacc ggcgacgttg gtgtgatagg agatcacact
      781 cagcgggccg cctccgccat tgacgaactg cagcgacagc gggccgcgcg aattgaggta
      841 atacgcaccc acgacccact gcagcggtcc aggaccggtg gacagcaggt tgaattcctg
      901 cgtgaaggtt ctgaagtcag cgcgtcgcac cagatagacg tcggccggcg cggatgaggc
      961 atccacatcg gtcagatcgc tctcgtaacc gctctggtaa ctgctgacgg atcgcagttc
     1021 gacatcgggc gtcatgtgcc agatgccggt accggtcgag cgctgaccgg agatgttgta
     1081 gtactgattg cgatcgtagt cgatcgtgaa agcctggttc tggatggctg cagccgtcgg
     1141 gtcggcaaac cgcgtggtcg gaaccttgaa gtaggcgccc agcggcccga tcgcggcctc
     1201 atccggcttg accgcaaagc cgtcggtacg gctcgaatac ggctcgtagc gcagcgtgta
     1261 ttcgaagtcc tcgttgggcc ggaaggccag ctgtgcgcgc accgcattga aattgatgtt
     1321 tcccggctga ttttgcgccc ccgggaatcc ggccgcggca gcgatcggcg agctggcctg
     1381 ggcacccaga ttggtcgtga aactatcgcg cgtctcgtag acaaaggcca cgcgtgccgc
     1441 gaacatgtcc gacagcggca ggttgactgc gccctcggtg cggcgatcgt tgtagttgcc
     1501 gaatgtctgc tcgaggtagc ctttgagcgc gtcgaacgac ggctgcacgc tgttgacaaa
     1561 gatggcgccg ccatccgaat tggaaccgac cagcgtcccc tgcgggccgc gcagcacttc
     1621 gacatcctga atgtcgtaga acgtgtcgac cgtatcgatc agcgacggga tggcctgtcc
     1681 gtcgacgtag aaggcgacac cgttggatga ggcgggattg gtttgctgga tgcccacgcc
     1741 gcggatgttg atgaaggtct cgctgctcag tggcggctga atcgtcagtc ctggtgtctc
     1801 cagctgcagg tcctggatgt tcagcacccc ttcctctttg agttcctcgc cgttgcgtac
     1861 cgtcgcggaa accgaggtgg tctgcagatt ctcagatttc ttctccgcgg tgatcgtcac
     1921 ctcggtcagg acggcctcgg tcggggcggc acccgtgccg gcagtggtct gtgcctgact
     1981 cggcgccggg ctcatgagcc cgacgcccag aatacatgca ctggaagcaa cgccgtaaag
     2041 cgccggtctg ccaatacgcg gccgtaaatc tctcctgcga gtactcattt gccccctgat
     2101 ccttcgaccc gttccatcga gtcgtatatt ggttgatgcc atcaactata gtcgaatcgg
     2161 taaacttggc aagtaagaaa ttcaatgtga ccataaaccc attatcttcc gcgctttgag
     2221 cgcagtgcgg caccggcgtt cgatggcgct tcaaggaacc ttcatggcta agttcgatga
     2281 tctcttctac cattcggcta cgggggtatc gatgattaac cgaagaatcc tgacccaggg
     2341 aatattgggt gccggcgtcg cggcactgac gacgggccac cggatcgtgg cgcaggagca
     2401 acgacagggc gaggcggtcg cgaagacgac gctgggttcg atccgcggtg cctctcagga
     2461 cggcatactg agcttcctcg gtgtgcctta cggcgctgcg ccgaccgggg acttgcgatt
     2521 catgccgccg cgtagcgccc aaccctggac cgacgtgcgc gacgcacgag cctatggcga
     2581 tacctgcccg cagataccgc tgggcctgtc cccatttgtg cgacatggcg acgggccccc
     2641 gccgccaccc tcgccgatgc agcgacagct cggcacgctg ttcacgcgcg gtgccaagga
     2701 gcccgcgcag agcgaggatt gcctggtgct gaatgtgtgg acgcgtgggc tggagcatgg
     2761 cagacggccg gtcatggtat ggctgcacgg tggcggcttc gccgtcggtt ccggttcgct
     2821 tccgacctac aacggagccc gactcgccgc ccgcggcgac gtggtgatcg tgacgatcaa
     2881 tcaccgactg aatgtcttcg ggcatctgta tctgggcgaa atcgccggcg agcgcttcgc
     2941 gcaatcggcc aacgttggaa tgctcgatat cctggcggcg ctgcagtggg tgcgcgacaa
     3001 catcgccggt ttcggtggcg atccgggaaa cgtcacgatc ttcggcgagt ccggtggcgc
     3061 cggaaaggtg agcgtgatgt gcgccatgcc ctctgccaga ggcctgttcc acaaggccat
     3121 catgcagagc ggtccgtgcc ttcagatcat cgagaaggcg cgcggcaccg cgatcgcccg
     3181 tcagctgctg caggatctcg ggctatcgcc caatcaggtg gtcgagctgc agcacatgga
     3241 tgcgatgaag ctggcaggcg cggcagaggc cgctgaagtc aaagtggtgc cgcgtattct
     3301 cggtttcggg ccgaccggtc tgatcccgtt ggtggacggc gtggtgttgc cacatcaccc
     3361 gttcgacaaa gtcgcctcac ccgaatcggc ccatgtcccg ttcctcgtgg gctcgaccaa
     3421 ggatgaggcc gtgctgttca ccggcccgct gccgcgctgg gggcaattca ccgatgacga
     3481 gttgctcgcg ctgctaggtc cgatcgcggg cgcccgctcg cgcgaggcat tgacgctgta
     3541 tcgcaggctc cacccggccg actcgaattc atacctgctg gccgacatgg tgaccgactt
     3601 ctggatgcgg caggccgcaa accatgtggc cgaattgaag gcgcagcagc acagcgctcc
     3661 ggcctatcag tacgttctgg aatgggagat caatcaggac ctgcgcacgg cgcatggcac
     3721 cgatgtcagc ctggtgttcg acaacgttgc ggcctcggcc gcggtcagcg gcgcacccaa
     3781 cgcgcaggcc gtgtccgatc agatgagtga tgcctggctg gcgtttgcca agaccggcaa
     3841 tcccaaccac gcgcgtctag cgcactggcc ggcgtattcg ctgccagcgc gggcgatgat
     3901 gttgttcaac gtcaacagtc acgtcatcga cgactacgag gcaccagcac gcgccttctg
     3961 ggaggcgcga tgacaaacag tcatgaccgc ttgcagaggc ggaccgtgcg acatactctt
     4021 gccggatctc atcaggggga gttcatagaa tgagatccac actcgccgtc accaccgtcg
     4081 cgatcctgtc gttgctgctc gcgcccacgc tgccggcgca ggctcccggt gcaccgggca
     4141 atccaggcac ggccagtctg ctgcccccgc cggacgcacc accgatcatg ccggcaccgc
     4201 ccggcccgta cccgatcacg atcgaggcca acgccgctct gcccacgcac accctctatc
     4261 gcccggccga tctcacaccc ttcaacggtc cgaagcggct tcccatcatc gcgtggggca
     4321 atggcgcgtg ttccaacgcc gggctgctgt tcaaggaatt cctgagcaac atcgcctcgt
     4381 acggattcat gatcatcgtg tccggcccga aggatgcgcc gctgcccgcc ttcgcgcgcg
     4441 ccgcacagcg cgcagcatca agctccgggt ccgctgcgcc accgccgacc ccgcccgcac
     4501 ccggcacccc gcggccgccc atgaccaccg atgcggacat gcaaaccgcg attgactggg
     4561 ccatcaggga aaacggcacg gcccgcagcc cctatcacgc aaagctcgat ccgcagaaga
     4621 ttgcggtcat gggtcagtcc tgcggcggcc tgcaagcgat cgccaattcg accgacccgc
     4681 gcgtgaaaac aagcatcatc tggaacagcg gtctgtttcc ggacggccag atcggccatt
     4741 cgctcagtgg tgccaccaaa gcgacgctgg cgaagattca tgcgccgatc gcctacatca
     4801 acggtgggcc cgcggatgcc gcctacgtca acacgctcga tgacatccag cacattgagg
     4861 gggtgccgct gttttttggc tggatcaacg tcggtcacgg cggaacctat acgcatccgg
     4921 gcggcgggcg tttcgccgaa gtggctgtcg cctggctcaa ctggcggctc aacggcgaca
     4981 agcaggcagc gaagatgttc gacggacccg attgcacgct gtgcaaggac cccatctggc
     5041 acgtgcagaa gaagaatatg aattgatcga tcggcgcgcg cactagctgg agtggccgtt
     5101 cagtcctcgc gacggaattt tccaggccag aaccagcgcg cacaggctgg cgatgatcgc
     5161 gatcggcagc agatccatca gcagctgcga gtagccgtgg ccggcggctc gaagcatgcc
     5221 gcccagcttc gggcccacga ttgaaccgat gcgcccgatc gccaccgccg cacccactcc
     5281 cacgccgcgg atgctcgtcg gatacaaggt cggtgccatc gcgtacagaa aggcctgcgc
     5341 cgcgagcacc gcgcatccga gcaggcacac cacgctcacc acaatcgaca gctgcgcggg
     5401 tgatcgggcc agcgccacca gcagaatcgg gaccatcacg aacgtgacga ggatactggg
     5461 cgtgcgctga cggccttcca gcagacca
//
LOCUS       DATMRM010000157         2669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4442570, whole genome shotgun sequence.
ACCESSION   DATMRM010000157 DATMRM010000000
VERSION     DATMRM010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4442570"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VNZ06_04710"
     CDS             <1..215
                     /locus_tag="VNZ06_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20084.1"
                     /translation="TLYREGDPNGLNAPKMLSVGLHCRLIGRPGRIAALERFIDYVRQ
                     HDHVWICRRVDIARHWIATHPYRPAL"
     gene            212..1297
                     /gene="uraD"
                     /locus_tag="VNZ06_04715"
     CDS             212..1297
                     /gene="uraD"
                     /locus_tag="VNZ06_04715"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HWW20085.1"
                     /translation="MIELTALNALQPRQFVQALSGIFEHSPWIAQRAAALRPFVSRLD
                     LLDAMRRIVLEASPDEQLALIHAHPRLGARGRSRQQLTQASSTEQRRAGLDACSDQEF
                     AHLERINASYLARFGFPFILAVRGHDPASIIAQLERRLGHDRAQEIATAVQQIGLIAS
                     YRLADTVASAPQKEVRAMLERLPGIDAASLLREWMLAASLSVSQTDASTLVGTLVCAP
                     PAEGTLIIGVHCNANTGSLSYDGQLGCACAIAVVQQLKSEASRLAFDVAILARPADPT
                     RGYVRALTGLDARCTRPLSRIDQLLPESQPMAGALARAQLLRGIVAVVPEGAAQTELE
                     TETETELTLARATGALQEFLLHTDCHG"
     gene            1290..2264
                     /gene="alc"
                     /locus_tag="VNZ06_04720"
     CDS             1290..2264
                     /gene="alc"
                     /locus_tag="VNZ06_04720"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HWW20086.1"
                     /translation="MADTNDLNLADARLGAVVLSATDEFFGAKERLLNPAEPQWRAGV
                     YDDHGKWMDGWETRRKREHGFDHCIVQLAAPSTLHALDIDTRYFTGNYPPHASVEACH
                     SAGKPDASTRWSELLAYSALQGNQRNLFSLQSSAIHTHLKLNIHPDGGVARLRAYGAV
                     QRDWSRAASSDVIDLAAALNGGRALICSDEHYGVMGNLLLPGRGVSMADGWETRRRRE
                     PGFDWVVLRLGAAGHIERIEIDTAHFKGNFPHQVSVHAALLAAGEESDLASECLYWPL
                     LLEPQLLKADSVHDYRTQLRDVGPVSHVRVNIHPDGGLSRVRLFGRIS"
     gene            2284..>2669
                     /locus_tag="VNZ06_04725"
     CDS             2284..>2669
                     /locus_tag="VNZ06_04725"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HWW20087.1"
                     /translation="MSEIVLSAVPLSAQAFAPFGDVIETEGRGWRWVNQGTSQRFDDL
                     ARVDVLESGGRPLISIFRASPQPLPFDVRSLERHPLSCQSFYPLDARPFLVIVAEQGS
                     APIEQRIRAFMSSGRQGVNYHRNTWH"
BASE COUNT          438 a          863 c          883 g          485 t
ORIGIN      
        1 atacgctcta tcgtgaagga gatcccaacg gactgaacgc gcccaagatg ttgtcggtgg
       61 gcctgcactg ccggctgatc ggccggcccg gtcggatcgc ggcactcgag cgtttcatcg
      121 actacgtgcg acagcacgat catgtctgga tctgtcgccg ggtcgatatc gcgcgacact
      181 ggatcgcgac ccacccgtat cggccggcgc tgtgatcgag ctgaccgcac tcaacgcgct
      241 gcagccacgg cagtttgtac aagccctcag cggtatcttt gagcactccc catggattgc
      301 gcagcgagcg gccgcactgc gcccgttcgt gtcgcggctg gacctgctcg atgcgatgcg
      361 ccgcatcgtt ctcgaggcga gccctgatga gcaactggcc ctcatccacg cacacccgcg
      421 actgggtgcg cgcggtcgct cccgtcagca actgacacag gcttcctcca ccgagcagag
      481 gcgggcgggc ctggacgcct gcagcgatca ggaattcgcg catctcgagc gcatcaatgc
      541 ctcctatctc gcaaggttcg gcttcccgtt cattctggcg gtgcgcggcc atgatccagc
      601 gtcgatcatt gcccagctcg agcggcggct gggacatgac cgggcgcagg agatagcgac
      661 ggccgtccag cagatcgggc tcattgcgag ctaccggctc gcggacacgg tggccagtgc
      721 gccgcagaag gaagtccggg ccatgctcga gcgcctgccc ggcatcgatg cggcttcact
      781 gctgcgtgaa tggatgctcg ctgcgagcct gagcgtctcg cagaccgacg ccagcacgtt
      841 ggtaggaacg ctcgtctgcg ccccgcccgc agagggcacc ctgatcatcg gtgttcactg
      901 caacgcgaac accgggtcgc tcagctacga cgggcagctg ggctgcgcct gtgcgattgc
      961 ggttgtccag cagctcaaga gcgaggccag tcgcctcgcg ttcgatgtcg cgattctcgc
     1021 ccgacccgcc gatccgacgc gaggctacgt ccgggcgctg accgggctgg atgcgcggtg
     1081 cacccggccg ctgtcacgga tcgatcagct tcttccggag agccagccga tggcaggcgc
     1141 gctggcgcgg gcgcaactgt tgcgcgggat cgtcgcggtg gtgccggaag gcgccgcgca
     1201 gacggagctg gagacggaga ctgagactga attgacgctc gctcgcgcca caggtgcgtt
     1261 acaggaattc ttgctgcaca ctgattgcca tggctgacac gaacgatctg aatttggccg
     1321 acgcgcgtct gggcgccgtc gtgctgtcgg ccaccgacga gtttttcggg gcgaaggaac
     1381 gtctgctgaa tcccgccgag ccgcagtggc gcgccggcgt ctatgatgat cacggcaagt
     1441 ggatggacgg ctgggagacg cggcgcaagc gcgagcacgg cttcgatcac tgcatcgtgc
     1501 agctcgccgc gcccagcacc ctgcacgcgc tggacattga tacgcgctat ttcacgggca
     1561 actatccgcc gcatgcctcg gtcgaggcct gtcattcggc cggcaagccc gatgcctcca
     1621 cccgatggag tgagctgctg gcctactcag cgcttcaggg caatcagcgc aaccttttca
     1681 gcttgcagtc ctcagcgatc catacgcacc tgaagctgaa catccatccg gatgggggcg
     1741 ttgcccgatt gcgcgcctac ggggctgtgc agcgcgactg gagtcgcgcc gcttccagcg
     1801 atgtgattga tctggccgcc gcgctcaatg gcggccgcgc actgatctgc agcgatgagc
     1861 actacggcgt gatgggaaat ctgctgctcc cggggcgggg cgtgagcatg gccgatggct
     1921 gggaaacccg tcgtcgccgc gagccgggtt tcgactgggt ggtcctgcgc ctgggggctg
     1981 ccgggcacat cgagcggatc gagatcgata ccgcgcattt caagggcaat ttcccgcatc
     2041 aggtatcggt gcatgctgcc ttgcttgcgg ccggcgaaga atcggatctc gccagcgagt
     2101 gcctgtattg gcccttgctg ctcgagccgc agttgctcaa ggctgatagc gtgcacgact
     2161 atcgcacgca gctgcgtgac gtcggcccgg tctcgcatgt acgcgtcaat attcacccgg
     2221 acggcggcct gagccgggtg aggctgttcg gccgtatcag ttagcgtttc gcgaagggga
     2281 gtgatgtcgg agatcgtact ctcggccgtc ccgctcagtg cgcaggcgtt cgcacccttc
     2341 ggcgatgtca ttgagaccga aggcaggggt tggcgctggg tcaaccaggg tacgagccag
     2401 cggttcgacg atctggcgcg ggtggatgtg ctggagagcg ggggccgacc gctcatcagc
     2461 atcttccggg cgagtcccca gccgctgccg ttcgacgtgc gctcgctcga gcgccatccg
     2521 ctctcctgcc agagcttcta tccactcgat gcacgcccgt tcctggtgat cgtggccgaa
     2581 cagggcagcg cgccgatcga gcagcgcatt cgcgccttca tgagctcggg ccgccagggc
     2641 gtcaactatc atcgcaacac ctggcacca
//
LOCUS       DATMRM010000158         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1024478, whole genome shotgun sequence.
ACCESSION   DATMRM010000158 DATMRM010000000
VERSION     DATMRM010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024478"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..1905
                     /locus_tag="VNZ06_04730"
     CDS             253..1905
                     /locus_tag="VNZ06_04730"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HWW20088.1"
                     /translation="MYFRRKQSQGRIYLQIVESHRTGDQVRQRVIATLGRLDELEASG
                     QLDRLLRSGARFVQQSMVLDAARTGEVPAITVRRIGPALLFERLWAETGCQSVITALA
                     RRRGHRFGLERAVFLTVLHRLMRGGSDLAADRWREEYRIAGSEDLDLHHLYRAMAWLG
                     EELPADQQDGATRFAPRCSKDLIEEHLFARRRDLFSRLDLVFMDTTSLYFEGLGGQSI
                     GQYGYSKDHRPDLRQMILVVLIDGDGRPVCSEMWPGNTADVTSLVPVIDRLRRRFAIG
                     RVCVVADRGMISAETIAALEQRGLLYILGVRERTDKLVRDVVLNDAAPFIPLIIEKRG
                     RDTDYSAKAVTLGGVRYIVCINHQEAAKDAADRTAILTALERQLRRGDKALVGNTGFR
                     RFLKTVGDGHFAIDHSKADEDARFDGVFVLRTNTDLNPLAAMLRYKQLWAVEQTFRTA
                     KHLLATRPIFHKLDQTIRGHVFCSFLALVLKAELEARIAALGQNASWPAIIANIDALT
                     ETDVEQDGKRFLLRSTPRIAAGLALRAAGVALPPTVQAIAEH"
     gene            2151..2354
                     /locus_tag="VNZ06_04735"
                     /pseudo
     CDS             2151..2354
                     /locus_tag="VNZ06_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2469..>2748
                     /locus_tag="VNZ06_04740"
     CDS             2469..>2748
                     /locus_tag="VNZ06_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20089.1"
                     /translation="MTTGIDPQSLEQQRGADVAHRDRGGLAAPGRVEHHRLLHKAGAR
                     AQQSIELSAGFEFIQPSKRSDHALAHLLAAAVALHDLQVDASLRLFAAK"
BASE COUNT          496 a          899 c          844 g          509 t
ORIGIN      
        1 tccaattacg gtcatgcatt ttactcagta gtcggcaagt aggacgacat gccgatgagc
       61 ttatccactc gcaccctgat gcttccaacc catcttcgac agtcgggtgt tcgtggcgcg
      121 ggctaagtga ctgaattcat tggccgtggc tggcgttttc gataaatgtg tagtgccacg
      181 cgctagtggt tttctccgtt gactacatgc gtatcttcgg caatctgtgc gcacccaaac
      241 gccgattcgc acatgtactt ccgccgcaag cagtcgcagg ggcgcatcta cctgcagatc
      301 gtcgaaagtc accgtaccgg cgatcaggtg cgtcagcgcg tgatcgcgac actcggacgg
      361 ctggatgaac tcgaggccag cgggcaactc gatcgactgc tgcgctcggg cgcgcgcttc
      421 gtgcagcaat cgatggtgct ggatgcggcg aggaccggag aggtgcccgc gataacggtg
      481 cgtcgcatcg gtccggcact tctgttcgaa cgactttggg cggagaccgg ctgtcagagc
      541 gtgatcaccg ccttggcgcg ccgccgcggt catcgcttcg gcttggaacg tgcggtgttc
      601 ctcaccgtgc tgcaccggct gatgcgtggc ggctcagatc tggcggctga ccgctggcgc
      661 gaggaatatc gcatcgccgg cagcgaagac ctcgatctgc atcatcttta tcgcgccatg
      721 gcctggctcg gcgaggaact gccggccgat cagcaggacg gcgcgacgcg gttcgcacca
      781 cgctgcagca aagatctgat cgaggagcac ctgtttgcgc gtcggcgcga cctgttcagc
      841 cggctcgacc tggtgttcat ggacaccacc agtctgtact tcgagggcct gggtggccaa
      901 tcaatcggac aatacggcta cagcaaggac catcggcctg acctgcgtca gatgatcctc
      961 gtcgttctga tcgacggcga cggacgcccg gtgtgctctg agatgtggcc cggcaacact
     1021 gccgacgtca ccagcctggt gccggtgatc gaccggctgc gccggcgctt cgccatcggc
     1081 cgcgtctgcg ttgtcgccga ccgcggcatg atcagtgccg agaccatcgc cgcactcgag
     1141 cagcgcggcc tgctctacat cctcggtgtg cgcgagcgca ccgataaact ggtgcgcgat
     1201 gtggtgctga acgatgctgc gcccttcata cctcttatta tcgagaagcg tggcagagac
     1261 accgactata gtgccaaggc agtcacgctc ggcggggtac gctacattgt ctgcatcaac
     1321 catcaggagg cagcgaagga cgctgccgac cgcaccgcca ttctgaccgc gctggagcgg
     1381 cagttgcgcc gcggcgacaa ggcgctggtc ggcaacaccg gcttccgtcg cttcctcaag
     1441 accgtcggcg acggtcactt cgcgatcgat cacagcaagg ctgacgaaga cgccaggttc
     1501 gacggcgtgt tcgtgctgcg caccaacacc gatctcaatc cgctggccgc catgctgcgc
     1561 tacaagcaac tctgggccgt cgagcagacc ttccgcacag caaagcatct gctcgctact
     1621 cgaccgatct tccacaagct cgaccagacc attcgcggac acgtattttg tagtttcctt
     1681 gccctcgtac tgaaggccga actcgaggcc cgcatcgccg cgctcggcca gaacgcatcc
     1741 tggcccgcca tcatcgccaa cattgatgca ctgaccgaga ccgacgtgga gcaagacggc
     1801 aagcgcttcc tgctccgatc aacgcccagg atcgctgccg gcctcgcact gcgtgccgcc
     1861 ggcgtcgcac tgccaccaac cgtgcaggct atcgcggaac actgattacg aactaccaac
     1921 atgtagtgcc acgccgcccc agcggcgcca attgtcacgc gctttcaatt acttgcggaa
     1981 gggcactgtc gaagatgggt cagagcgtga tcaccgcctt ggcgcgccgc cgcggtcatc
     2041 gcttccggtt ggaacgtgcg gtgtttctca ccgtgctgca ccggctgatg cgtggcggct
     2101 cagatctggc ggctgaccgc tggcgcgagg agtatcgcat cgccggcagc gaggctcttg
     2161 acctgcacca cctataccgg gcgatggcct ggctcggcga ggaactgccc gccgatcagc
     2221 aggacggcgc cacaccgttt gcgccacgct gcacgaaaga cctgatcgaa gagcaactgt
     2281 tcgcgcatcg gcgcgatctg ttcagccggc tcgacttggt gttcatggac accaccagcc
     2341 tgtatttcga gggcctcggc ggtcaatccc gagccgccgc gcatcagccg atgcagcacg
     2401 gtgaggaaca ccgcgcgttc cagcgcgaag cgatggccgc ggcggcgcga taaggcggcg
     2461 atcacgctgt gacaaccggt atcgacccac agtctctcga acagcagcgc ggggccgatg
     2521 tggcgcaccg tgaccgcggg ggcctcgccg ctcctggccg cgtcgagcac catcgcctgc
     2581 tgcacaaagc gggcgcccga gcgcagcaat cgatcgagct gtccgctggc ttcgagttca
     2641 tccagccgtc caagcgtagc gatcacgcgt tggcgcacct gctcgccgct gcggtggctc
     2701 tccacgatct gcaggtagat gcgtccttgc gactgtttgc ggcgaaag
//
LOCUS       DATMRM010000159         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3485991, whole genome shotgun sequence.
ACCESSION   DATMRM010000159 DATMRM010000000
VERSION     DATMRM010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3485991"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="VNZ06_04745"
     CDS             <1..1277
                     /locus_tag="VNZ06_04745"
                     /inference="COORDINATES: protein motif:HMM:NF027518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HWW20090.1"
                     /translation="GDALLHPNRGIDTRAIGWDKQLAGGRLPQHYSFAGVRLMSGVFA
                     VIGAVVFFAVMLALTRSVLAALLLSNLYVFENAFIAHFRAAHLDAFQLAFALAALLCF
                     VVSARRGRRSSPWLEFTLGAACGLAIMVKLNAAVWTLLGLMLILRRIGMGWHCAPRAR
                     LLLTGMRDGAVIASGCLLAIVVVWTIYVAVTPHLLPPGSPAGARDEAFMSHTYRAYLE
                     GARGFSPMVVLAAAQDYTRFMLADLKGVPKNDRNGSQPMEWPRHIRTINYRWDSDGVH
                     TAYVQLVGNLWGWLMAGIALVATVGLLILQSWRPCPSSDPERRALMVMLLLQYLVFMT
                     AHAYLGTMRVMYLYHYFLALVLAFCLVPLVLAEAAERWPALRAWHELELAVITVLLLA
                     SFAFYSPLSFHSPLTHAQCEWRNAVQHIVDCR"
     gene            complement(1360..1920)
                     /locus_tag="VNZ06_04750"
     CDS             complement(1360..1920)
                     /locus_tag="VNZ06_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20091.1"
                     /translation="MSKLMYALLGAGALVAVASLGSRFVVADTLSGSSSSSSTSSSTS
                     SSSTSSSSSGSGPDCSGAAAGLGVIWPPDHTMVAETIVGVTDAFGLQMTITVTGIQQD
                     EPVNALGSGNTAPDGSGVGTPTASVRAERAGPGTGRLYFISFSATDTSGMQCSGMVQA
                     FVPHDQGQGFTPIDTGLRYDSTVVPN"
     gene            complement(2222..3328)
                     /locus_tag="VNZ06_04755"
     CDS             complement(2222..3328)
                     /locus_tag="VNZ06_04755"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWW20092.1"
                     /translation="MPTRALELSVVVATYQERNNIPELVSRIERALGALTWELTVVDD
                     DSPDKTADLVREFARRDPRVRCVQRIGRRGLASACMEGMLSSAGPAIVVMDADLQHDE
                     TQIPIMLSQLLSGATDIVIASRYVNGLDVGYWEGRRVAISLWANRLGRLVCRQAISDP
                     MSGFFMIRRSVLDSTVHKLSGIGFKILLDILTSAPKPLCVREVPYTFRPRFAGQSKLD
                     GMVAWEFGMLILDKFTRGAIPARFIAFTIVGAAGVAVHMTVLTLLLKALSSPFYYSQA
                     VATVAAMIFNYAVNNVLTYRDRRLRGWSWVSGLLTFMLACGIGALANVGLAAYLFERQ
                     SRWFIAALCGIAVSAVWNYAVTRLFTWRDVATAR"
BASE COUNT          633 a         1019 c         1040 g          747 t
ORIGIN      
        1 ccggtgatgc actgcttcat cccaatcgcg gcattgacac gcgcgcaatc ggctgggaca
       61 agcagctcgc aggcgggcgt ctgccgcagc actactcgtt cgcgggcgtg cggctgatgt
      121 ccggagtgtt tgcggtgatc ggcgccgtgg ttttctttgc tgtaatgctg gctctcacgc
      181 gctccgtgct tgcggcgctg ttgctcagta atctgtacgt gtttgaaaat gcgttcatcg
      241 cgcattttcg agcggcgcac ctcgatgcgt tccagcttgc gttcgcgctg gctgctttgc
      301 tgtgtttcgt tgtgagtgcg cgccgtggcc ggcgcagttc gccttggctg gagtttaccc
      361 tgggcgcagc ctgcgggctg gccatcatgg tcaagctgaa tgcggcggtg tggaccctgc
      421 ttggcctgat gctgattctc cgccgcatcg gaatgggctg gcattgtgct ccgcgagcgc
      481 gtctgctcct gacgggcatg cgcgatggcg cggtgatcgc ctccggctgc ctgctggcca
      541 tcgtggtcgt gtggacgatt tacgttgcgg tcactcctca tctgctgccc ccgggcagtc
      601 ccgccggtgc aagagatgaa gccttcatgt cgcataccta tcgggcgtac ctggagggtg
      661 caagaggttt ctctcccatg gtggtgttgg ccgcggcgca ggactacaca cgcttcatgt
      721 tggctgactt aaagggtgta cccaaaaacg atcggaatgg gtcgcaaccg atggaatggc
      781 cgcggcatat ccgcacgatc aattaccggt gggactcgga tggtgtacat acggcgtacg
      841 tgcaactggt cggaaatctt tggggatggt tgatggcagg catcgccctc gttgccacag
      901 tcgggttgct gatcttgcag tcgtggcgtc cgtgtccgtc gtccgacccg gagcgccgcg
      961 cactgatggt catgctactg ttgcagtact tggtgttcat gaccgcacac gcctatctgg
     1021 gaacgatgcg ggtcatgtat ctgtaccatt atttcctggc gttggtcctt gccttctgcc
     1081 tggtgcccct cgttttggcc gaggctgcgg aaagatggcc agcactgcgc gcgtggcacg
     1141 aattggagct ggcggtaatt acagtgttgt tactggccag tttcgcgttc tattcgccgt
     1201 tgagctttca cagtcctctg acgcacgctc aatgcgagtg gcgcaacgcc gtgcagcata
     1261 tcgtggactg tcgctgaggc gagctctgca cggcagctcg gcctgcgaac cgctgccggg
     1321 aaggcgcagc ggcccgcagg ccgggtttac tttaggcagt caattgggta cgacggtcga
     1381 atcgtagcgc agtccggtgt ctatcggcgt gaatccctga ccctggtcgt gtggaacgaa
     1441 tgcctgcacc attccgctgc actgcatgcc cgacgtgtcg gttgcgctga atgagatgaa
     1501 gtagaggcgg ccggtcccgg ggcctgcgcg ctcggcgcgt acggaggcgg tcggggtgcc
     1561 tacgcccgag ccatcgggcg cggtattgcc gcttccgagc gcgttgaccg gctcgtcctg
     1621 ctgaatgccc gtgaccgtga tggtcatctg gagaccgaaa gcgtccgtta cgcccacgat
     1681 ggtctctgcc accatcgtgt ggtccggcgg ccaaatgacg ccgagccccg ccgctgcccc
     1741 actgcagtcc gggccggatc cgctgctcga actactggtc gagctgcttg aagtgctgct
     1801 cgaggtgctg ctggaactac tgctgcccga cagcgtgtcc gccacgacga atcgagagcc
     1861 gagcgaggca acggccacca gcgcgcccgc gccaagaagt gcatacatga gtttagacat
     1921 taacctctcc tcccgatttt gtcggccgca cgccgacgca cgtctcaatt tgatgcgacg
     1981 ccatttccgg cgatcgggtc tatccaggtc aagaggtccc actggcagcc gaaccagaac
     2041 gtgacatggg tcgcattcgc gtctgcattg cagacataac actgtcgcga agcgactaca
     2101 gtctgcaggg gacttgccgg ggtcgacgcc tttcagcagg gcgcgcgtga gctttggcga
     2161 aggcagtgag ctccggccag ttgtgaaatc gaagcgctcg gggtttggcg tgcggctcag
     2221 tctaccgggc ggtcgctacg tcgcgccaag taaataggcg cgttacagca taattccaca
     2281 ccgcgctcac cgcgatcccg cacagagctg caatgaacca ccgagattgc cgttcaaaga
     2341 gatacgcagc tagccccacg ttcgcgaggg caccgatccc acacgccaac atgaacgtca
     2401 acagtccgct gacccacgac cagccccgca gccgtcgatc gcggtatgtc aatacattgt
     2461 taacggcata attgaagatc attgcggcga cagtcgcgac agcctgactg tagtaaaacg
     2521 gtgacgatag cgctttcagc aacaaggtta ggacagtcat atggacggcg acgcccgctg
     2581 caccgacgat tgtgaatgcg ataaacctgg ctgggatggc gccacgagtg aacttatcaa
     2641 gtatcagcat gccaaattcc cacgctacca tgccgtcgag cttgctttgt ccggcaaagc
     2701 gtggccggaa ggtgtacgga acctcgcgaa cgcagagggg tttcggggca gacgtcaata
     2761 tatccagcag aattttaaag ccgatgccgg atagcttatg taccgtgctg tctagcaccg
     2821 agcgacgtat catgaaaaaa ccgctcatgg gatctgaaat cgcttgacgg cagaccaggc
     2881 ggcccagcct attggcccaa aggctaattg cgactcgccg gccctcccag taaccaacat
     2941 ccagaccatt tacgtaccga ctggcaataa cgatatcggt agcgccgctt agcaactggg
     3001 aaagcatgat gggaatttgc gtctcgtcgt gctgcaaatc ggcgtccatt acgactattg
     3061 ccggtccagc actgctgagc atgccctcca tgcaggccga ggcaaggccg cgccggccta
     3121 ttcgttgaac gcaccggacg cggggatctc gccgtgcaaa ttctctaacc aggtctgcgg
     3181 tcttgtctgg tgagtcgtca tcaaccaccg ttagctccca ggtcagcgcc cccaatgctc
     3241 tttcaatccg gctgaccagc tccggaatgt tattccgctc ctggtatgtg gcaacgacaa
     3301 ctgacagttc cagtgcgcga gtcggcattc ttcattcaca cttactgagc tatcgcttcc
     3361 atgtgaaccg gcagtctgcc ctttgccagt cgcgcggatc cgtgatccag gacacggtta
     3421 gccggtttgt tgcctcttt
//
LOCUS       DATMRM010000160         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_711677, whole genome shotgun sequence.
ACCESSION   DATMRM010000160 DATMRM010000000
VERSION     DATMRM010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711677"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VNZ06_04760"
     CDS             complement(<1..416)
                     /locus_tag="VNZ06_04760"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWW20093.1"
                     /translation="MAVGCAAGPNFHPVAAPAVSGYAPEPLPPHTASADVIGGALQTL
                     RPGADIPGQWWTLFHSPQLNRLIEQALRANPDLQAAQAALREADENVSVQESYFYPTI
                     VAGVSGLRERRLTYTQGTPNRIASPYTLLNVGANVS"
     gene            complement(705..>2406)
                     /locus_tag="VNZ06_04765"
     CDS             complement(705..>2406)
                     /locus_tag="VNZ06_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW20094.1"
                     /translation="ALVGSLLFALTVVPALASLILRVPSNAEARGGESESAWFVELRD
                     RYGRSLDWVIAHRARVIAAAAMLLAVALGSLHFIGTEFMPRLDEGSIVVTSRRLPGIS
                     LTESISLGKQIERVTMSFPEIKGVVTKLGRPDLATEAMGIYESDSYLTLNPQSTWKCC
                     NSKEALIGRLSVALAAIPGVAYEFTQPMEMRMDEALTGIRGDVAIKIFGDDINALQDL
                     ARQVLSVISAVPGAFNPQMEQTSGVPEVQIEINRDELARYGLNVSDVREMIETLVGGT
                     QVSEMIIGQQRFAIALRLPETQRNDLDKLGNLLLHAAGGELVRLNQVADLNVVNSPEV
                     ITREDARRRIVVQTDVRGTDLGSFVAAAQARVSAAVNFPFGYSIVWGGQFENQQRADR
                     RLAIVLPVSIALIFALLFATFENLGQTFLILLIVPFALVGGIGALWLRGINLNLSASI
                     GFIALFGVAVLNGVVMVSHINLLRSQGLAIDRAVRLGASDRLRPVMMTALVASLGFIP
                     MAMATSTGAEVQRPLATVVIGGLITATILTLYLLPLLYPWFSPPEALPLASNEQMSKL
                     "
BASE COUNT          521 a          718 c          716 g          451 t
ORIGIN      
        1 taggacacat tcgcgcccac attcagcagc gtgtagggtg atgcgatacg gttgggagtg
       61 ccctgcgtat atgtgagtcg acgttcgcgc agccccgaga cgccggccac gatagttggg
      121 tagaaataac tttcctgcac gctgacgttc tcatccgcct cgcgcagcgc ggcctgcgcc
      181 gcctggagat caggattggc tctcagcgct tgctcaatca gccggttaag ctgcggggaa
      241 tgaaagagtg tccaccactg cccgggaatg tccgcacctg gccgcagtgt ctgtagagcc
      301 ccgccaatca catcggcaga agcagtatgg ggcggcaagg gctccggcgc gtagccgctg
      361 accgcagggg cagccaccgg atggaaattc ggtccggccg cgcagccaac tgccatgctg
      421 actataagaa agcatgccac ttcaaaatag cgtccggaca tattggtgca ccgagtcgac
      481 acatatcgca ccccaggtcc ctctaacgat accatgggag cactggattg ggctctcgaa
      541 tacgtaccgg taggcgtgaa gaatgccgtc acaagaggtg cggcaatcat cacggtggaa
      601 atatccagcg agggtggatg gtaatgtctg gggttgaatt tccgaacgcc gcggtatcct
      661 gacgaatgct tcggcagctc cagctcccag caacgtcgtt gctgctagag tttcgacatc
      721 tgttcgttac tggccagcgg cagagcctcg ggcgggctga accaagggta aaggagcggc
      781 agcagataga gagtcaggat agtggcggtg atgagtccgc cgatcactac tgtcgccagg
      841 ggccgctgaa cttccgctcc ggtgctcgtg gccattgcca tcgggatgaa gcccaggctc
      901 gcaaccaacg cggtcatcat gaccggacgc agccgatcgg aggcgccgag acggaccgcg
      961 cgatctattg ccaaaccctg gctgcgaagc aggttaatat ggctcaccat cacgacgcca
     1021 ttgagcaccg cgacgccgaa caatgcgata aagccgatcg aggccgagag gttgaggtta
     1081 atgccgcgca gccacagcgc cccgattccg ccgacgagcg cgaacggaac gatgagcagg
     1141 atcaaaaatg tctgccccag gttctcgaag gtagcaaaaa gaagggcgaa gatgagagcg
     1201 atagagacgg gcagcacaat cgccagtctg cggtcggccc gttgctgatt ctcgaattgt
     1261 ccgccccaga caatcgaata gccaaacgga aaattcaccg ccgctgagac cctggcctgg
     1321 gccgcggcga cgaaactgcc aagatcggtg ccacgcacgt cggtttgcac aacgatgcgc
     1381 cggcgggcgt cttcgcgcgt gatcacctcg ggactgttga ccacgtttag gtcagcgacc
     1441 tggtttagcc gcacaagctc gccgcccgca gcgtggagca gcagattacc gagcttatcc
     1501 aggtcgttgc gctgagtttc cgggagccgc agtgcgattg cgaaccgctg ctggccgatg
     1561 atcatctcgg agacctgagt gccgccgacc agcgtctcga tcatctcccg cacgtcagaa
     1621 acgttgagcc cgtagcgtgc cagttcgtcg cgattaattt cgatttgcac ttccggcacg
     1681 cctgaagtct gttccatctg cgggttaaat gctcccggaa ccgcggagat caccgacagc
     1741 acctgcctgg cgaggtcttg cagcgcgttg atatcgtcac cgaagatttt gatcgcgaca
     1801 tccccgcgta tacctgtgag cgcctcgtcc atccgcatct ccatcggctg cgtgaattcg
     1861 taggcgaccc cgggaatcgc cgcaagcgcg accgaaagcc tgccgatcag tgcctctttg
     1921 ctattgcagc atttccatgt gctttgtgga ttaagcgtca gatatgagtc cgattcgtag
     1981 atacccatgg cttcggtggc aaggtcggga cgtcccagct tggtcacgac tcccttgatc
     2041 tcggggaaac tcatggtgac acgctcgatt tgctttccga gactgattga ttcggtcaac
     2101 gatatccccg ggagacggcg cgacgtgacc acgatcgagc cctcgtccag tcgcggcatg
     2161 aactcggttc cgatgaagtg aagcgatccc aatgcgaccg ccagcagcat ggcggcggca
     2221 gcgattactc tcgcgcgatg agcaatcacc caatcgagcg aacgcccata ccgatctcgg
     2281 agttcaacga accatgcgct ttccgactcg ccgccacggg cttcagcatt ggacgggact
     2341 ctgagaatca gacttgcaag tgcagggacc acggtcagcg cgaatagcag cgacccgacc
     2401 aacgcg
//
LOCUS       DATMRM010000161         3089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3487803, whole genome shotgun sequence.
ACCESSION   DATMRM010000161 DATMRM010000000
VERSION     DATMRM010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3487803"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VNZ06_04770"
     CDS             <1..729
                     /locus_tag="VNZ06_04770"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWW20095.1"
                     /translation="PVNHRGGNAPSVVSVDHIGVPPGARVELIVTGPPLGTPGLFVTR
                     TVDTGQGGENDPNRALASITAASDAPEPHALPPQHPARLTRPALPWLGDVTPTRVRKL
                     YFSEKLENPNDPNSATAFYLTVDGQMPTPFDPSRQQPNIVVRQGDVEDWIIENRSNEL
                     HDFHIHQLHFLLLAWSGIAVKEPYLRDTVNVPFFTSRMLKFPSVRIRMDFRDPNSVGT
                     FVYHCHLLEHEDGGMMGTIRVEPR"
     gene            802..2778
                     /locus_tag="VNZ06_04775"
     CDS             802..2778
                     /locus_tag="VNZ06_04775"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWW20096.1"
                     /translation="MKHPAVRAASILLPIVLSSCGSGSAPSPTATPIQHVVIIIGENR
                     SFDHVFATYVPSGQQSIWNLLSQRIVNADGTPGPNFQQALQSAASDLASDAFLLNPPK
                     LAFPNNLLPAPMTGGPTDSFIPGDSLALAQASEPGLPAEYYPSLVSGGSGQMYGVPDA
                     RIANANALPPGPFQLTSSTFGYPSYAASPTHRFYQMWQQLDCALLHATPANPSGCDSA
                     LFSWVEVAVGIGSSGQPQPANFSIVYAPNAYTTGEGGTSMGFYNVQNGDVPYFKGLAD
                     QFAMSDNFHQSVQGGTGANHIMLGHGDMIWFSDGNGHAMTPPENMPSVTGFTAGRIVN
                     EIENPNPQPGTNDYYAQDGYFGGSYTNCADTTQPGVAPIVNYLQSLPRPIDPHCETGH
                     YYLLNNYNPGYFGNGNNAYTDTNPHNSGFIVPPSSIPGIGDTLLAANVSWKYYGEQWN
                     NYLPDPYQLNYSAIGANSDEYCPVCNPFQYDTSIMTQAAVRSAHLQDTSDLYRDIQSS
                     TLPAVSFVKPDGFVDGHPGSSKLDLFEGFVKKIVDAIQANPALWSNTAIFITFDEGGG
                     YYDCGYVQPLDFFGDGARIPFMVVSRYAKPGYISHEYTDHVSMLKFIERNWYLPTVSS
                     RSRDTLPAPKTDPSNPYAPTNSPALGDLFDFFAF"
     gene            complement(2737..>3089)
                     /locus_tag="VNZ06_04780"
     CDS             complement(2737..>3089)
                     /locus_tag="VNZ06_04780"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWW20097.1"
                     /translation="DEGGGYYDCGYVQPLDFFGDGTRIPLLIVSPYSTGGLVNHSYAD
                     HVSIIKFIERNWKLRPITRRSRDNFPNPRTSARNPYVPLNTPALSDLWDAFQFRKGGP
                     ASEGKEVEQIPQRR"
BASE COUNT          609 a         1023 c          898 g          559 t
ORIGIN      
        1 ccggtcaacc accgcggcgg caatgcgccc tcggtggtat cggttgatca catcggcgtg
       61 ccaccggggg ctcgcgttga gttgatcgtc accggtccgc cactcggtac gccggggctg
      121 ttcgtcacgc gcaccgtgga caccggccag ggcggggaaa acgatccgaa tcgtgcactg
      181 gcatcgatca ccgcggccag cgatgcacct gaaccgcacg cgctgccgcc acaacatccg
      241 gcgcggctta ccagaccggc gctgccgtgg cttggagacg tcacgccgac acgcgtcaga
      301 aaactctact tctcggagaa gctcgagaat ccgaacgatc cgaacagcgc cactgctttt
      361 tatctgaccg tggacggcca gatgccaacg ccattcgatc cgtcgcgtca gcagccgaac
      421 atcgtggtcc ggcaaggcga cgtcgaggac tggatcatcg agaaccgctc caacgagttg
      481 cacgacttcc atattcatca gttgcatttc ctgctgcttg cctggtcggg catcgcggtc
      541 aaggagccgt acctgcgcga tacggtcaat gtgccgttct ttacgtcgag gatgctgaag
      601 tttccgagcg tacgcatccg catggacttc cgcgatccga acagcgtcgg cacgttcgtg
      661 tatcactgcc atctgctcga gcacgaagac ggcggcatga tgggcacgat tcgcgtcgag
      721 ccgcggtagt ctgcggcgcg ccggcagcgc acgcggcgcg atgtctggcc tgctgtccgg
      781 caaggggcta cagtccggca gatgaagcac cctgccgtcc gcgccgcctc gatcctgctg
      841 ccgattgttc tgagctcctg cggttccggt tcagccccca gtccgaccgc caccccgatt
      901 cagcacgtcg tgatcatcat cggagagaac cgcagcttcg atcacgtgtt cgccacctac
      961 gtgcccagcg gacagcagag catctggaat ctgctatcgc agaggatcgt caatgccgac
     1021 ggcacaccag gtccgaattt ccagcaggcg ctgcagagcg cggcgagcga cctggccagc
     1081 gatgcgttcc tgctcaatcc gccgaagctg gcgttcccga acaacctgtt gccggcaccg
     1141 atgaccggcg gaccgactga ttcctttatc cccggcgaca gtctggcact ggcgcaagcc
     1201 tccgagccgg ggctgccggc tgagtactat ccctcgctcg tcagcggcgg cagcgggcag
     1261 atgtacggcg ttcccgacgc acgcatcgcc aacgcgaacg cactgccacc cggcccgttc
     1321 cagctcacca gcagcacctt cggctaccca tcctacgcgg ccagtccgac gcatcggttc
     1381 tatcagatgt ggcagcaact ggactgcgcg ctcttgcacg cgacgcctgc gaacccgtca
     1441 gggtgtgaca gcgcgttgtt ttcctgggtc gaggtcgcgg tcggaatcgg cagcagcggc
     1501 cagcctcagc ccgccaattt cagcatcgtc tatgcaccca atgcctacac cacgggtgag
     1561 ggtggcacat cgatggggtt ctacaacgtc cagaacggcg acgtgccgta cttcaagggc
     1621 ctcgccgacc agttcgccat gagcgataac ttccatcagt ccgtgcaggg cggcaccggc
     1681 gccaatcaca tcatgctcgg gcacggcgac atgatctggt tcagtgatgg caacggccat
     1741 gccatgactc cgccggagaa catgccttcg gttaccggat tcaccgcggg caggatcgtc
     1801 aatgagatcg agaatccaaa cccacagccc ggcaccaacg actactacgc gcaggatggc
     1861 tacttcggcg gctcgtacac caactgcgcc gacaccaccc agcccggtgt ggcgccgatc
     1921 gtgaactacc tgcagtcatt gccaaggccg atcgacccgc actgcgagac ggggcactac
     1981 tacctgctga acaactacaa cccgggctat ttcggcaacg gcaacaacgc ctacaccgac
     2041 accaatccgc acaacagcgg cttcatcgtg ccgccctcat cgatcccggg gatcggcgac
     2101 acgctgctgg ccgcgaatgt atcctggaag tattacggcg agcaatggaa caactatctg
     2161 cccgatccgt accagctcaa ctactccgcc atcggcgcca acagcgatga atactgcccg
     2221 gtttgtaatc cgttccagta cgacacctcg atcatgaccc aggccgcggt gcgcagcgca
     2281 cacctgcagg acacgagcga tctgtaccgc gacattcaga gcagcaccct gccggcggtg
     2341 tcgttcgtca aacccgatgg gttcgtcgat ggccatccgg gcagctcgaa gctcgatctg
     2401 ttcgaaggat tcgtcaagaa aatcgtggat gcgatccagg cgaatccggc gctatggagc
     2461 aacaccgcga ttttcatcac cttcgatgag ggcggcggct actacgactg cggctacgtg
     2521 caaccgctgg atttcttcgg cgacggcgcg cgcataccct tcatggtggt ctcccggtac
     2581 gccaagccag gatacatctc gcacgagtac accgatcacg tctcgatgct caagttcatc
     2641 gaacgcaact ggtatctgcc aacggtcagt tcgcgcagcc gcgacaccct gccggcaccg
     2701 aagacggatc cttccaaccc ctatgcgccg accaactcac cggcgctggg ggatctgttc
     2761 gacttctttg ccttctgatg cggggccgcc cttgcggaac tggaatgcgt cccacagatc
     2821 gctcagcgca ggagtgttga gcggaacgta ggggttgcgc gccgaagtcc tgggattggg
     2881 gaaattatcg cgactgcgtc gggtgatcgg cctgagcttc cagttgcgct cgatgaattt
     2941 gatgatcgac acatgatcgg cgtagctgtg attgaccaac cccccggtgc tgtagggcga
     3001 gacgatcagc agcgggatgc gggtaccgtc gccgaagaag tccagcggtt gcacgtagcc
     3061 gcagtcgtag tagccgccgc cctcatcga
//
LOCUS       DATMRM010000162         5422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2572564, whole genome shotgun sequence.
ACCESSION   DATMRM010000162 DATMRM010000000
VERSION     DATMRM010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2572564"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VNZ06_04785"
     CDS             <1..1201
                     /locus_tag="VNZ06_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWW20098.1"
                     /translation="GGAQSYPGFTPADQVDADRKNYAIYVDLTAKPIDPLRVDVAARY
                     EHYSDFGNATVGKLNVRYDLSPQFAIRGTVNNGFRAPTLAEEFYASTNVAPQFAFVQL
                     PPNSAGGKLLGVGNGLQPEKSINLGLGFVFRPIEHMTSTLDLFYIRLNNRIVGSASIP
                     GIAGGVVKSQAILNAIALNGNQLDPTVGLVGINVFANGIDTRTEGGDFTLNFPFDYGS
                     FGHVTYAVAIDYNDTSATRIPSTQLGGVALYDVEAISDVTTANPKYTIDLNAAWSNGP
                     LSVTLDEKIYGPASEYENDDADNGGGGPYPPSPLPSGFDYFRSSIGVTPITNLLVSYQ
                     LTKWLKISGGANNLFNRIPPTLNATLLAHEDNFFYGDNQGVQHYPDWSPFGINGGFYF
                     AKATVTW"
     gene            complement(1305..1832)
                     /locus_tag="VNZ06_04790"
     CDS             complement(1305..1832)
                     /locus_tag="VNZ06_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWW20099.1"
                     /translation="MKTRFKVALLLTLWPAVAALADDVKRYPLPNGNQFPIAQAVEVS
                     AGTTLIFHSGTVPSPKDPKAAAGSGDYWGDTRTQAMSVFNTLKASLDEKGLGFGDVIK
                     MTAFLVGDPAKQGRMDFQGFMDAYTQFFGTSSQPNLPARSAVQVAALAQPGIMVEVEV
                     ILARPPAKTGAKHKP"
     gene            1977..2516
                     /locus_tag="VNZ06_04795"
     CDS             1977..2516
                     /locus_tag="VNZ06_04795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWW20100.1"
                     /translation="MKSCPFQLRKARHADRPALQALIERSARELGAGDYRPEQIEAAL
                     RGAFGVDTQLIDDGTYFVVEADRKLVGCGGWSKRRTLFGGDAHQERDAQELDPKTDAA
                     KIRAFFVDPDYARQGIGRALLERCESEARTLGFSHFELMGTLPGVRLYQALGYVGSEP
                     VHYPVAPGVTIEFVPMQKA"
     gene            complement(2513..3616)
                     /locus_tag="VNZ06_04800"
     CDS             complement(2513..3616)
                     /locus_tag="VNZ06_04800"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HWW20101.1"
                     /translation="MKRVLALLTLCSLASIASAHVASTGFLAVHASGQELTGSIELAL
                     RDAELAVGVDSNNDGRVTWGELRAAEPRLQQYIQAHLGFDTEKSHCALQFQPIQVNQR
                     VDGNYAWVPFSAQCPLAIRRVRISYSLMAGVDPAHRGLLTLISGADAQTAVLGGRQSQ
                     QAFTLGESSGWRAFTEYLQAGIWHIWSGIDHLLFLLLPAVLLRRNGHWEPVSEVRPAF
                     INILKVVTAFTLAHSITLSLAALDVVRLPSRLTESVIAASIIIAALNNIFPLVTDSRA
                     RIAFLFGLLHGFGFASVLADMGLPHGARLISLVSFNLGIEVGQLAVVLSVMPLVYAVR
                     AGTLYRRTLMPWGSAAIAAIALVWFVQRAFFPT"
     gene            complement(3613..4761)
                     /locus_tag="VNZ06_04805"
     CDS             complement(3613..4761)
                     /locus_tag="VNZ06_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWW20102.1"
                     /translation="MKRTMMPLLLAALFIGLAPPVSAAPYIPGNDSQLLAELPQGVHH
                     AATSPTDLARRSVDVAIPLAQFYITRARASGDLRFLGYAENALDPWMKKTPVSPRVLV
                     LHATILQSRHDFADSLKELDQVLKRNPDDAQAWLTRATVLRVLGRYDEALDSCTHMAA
                     RSEPAVAMLCQQSLLALTGHLQSAYDTLEKLRFQVSTPELTAWRLSELGEMASHLGKD
                     DDAERWFRQGLQVAPDDFYLRAALSDLLLQHHRANETLQLLAGYETQEPMLLRIALAH
                     EALNDAQGEQARAYLGNAFDVEEQRGEAVHRREQARYLLDVEHQPGAALAAAQEDWKT
                     QREPADILILLRAAQAARQSDAAKPALQFIQQEHLEDVRLDAVKAALQ"
     gene            complement(4780..5055)
                     /locus_tag="VNZ06_04810"
     CDS             complement(4780..5055)
                     /locus_tag="VNZ06_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20103.1"
                     /translation="MKRQLVLLAAAAAATAFLAACGSSHPTTPVTSSGGMSSGGMSSS
                     SGVTVNTAQVLTLAQQSSETASPFSVDGGYVFTDTSETTNPISVDSH"
     gene            complement(5096..>5422)
                     /locus_tag="VNZ06_04815"
     CDS             complement(5096..>5422)
                     /locus_tag="VNZ06_04815"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HWW20104.1"
                     /translation="QTVGGEMLRLNTNTPVVASGSQNDLGVIAGDNAGYPNGRRPIDD
                     IVDITLRVAMGVLLQPYDGSAKDPDPASDMSRQFLYTDNVEAQPSDFLTAFPYVAPPV
                     PGSPDY"
BASE COUNT         1035 a         1732 c         1675 g          980 t
ORIGIN      
        1 cggcggcgcc cagtcctatc cgggcttcac cccggcggat caggttgatg ccgaccgcaa
       61 gaactacgcg atctacgtcg atctgaccgc caagccgatc gatccgttgc gggtcgacgt
      121 cgccgcccgc tacgagcact acagcgactt cggcaacgcc acggtcggca aactcaacgt
      181 gcgctatgac ctcagtccgc aatttgcgat tcgcggcacc gtcaacaacg gctttcgcgc
      241 gccgacgctg gccgaggagt tctatgcctc gaccaatgtc gcgccgcagt ttgcgttcgt
      301 gcaactgccg ccgaactcgg ccggtggcaa gctgctggga gtgggcaacg gcctgcagcc
      361 tgagaagtcg atcaatttag gacttggttt cgtcttccgg ccgatcgagc acatgaccag
      421 cacgttggat ctgttctaca tccggctcaa caatcgtatc gttggcagcg cctcgatacc
      481 gggaatcgca ggcggggtgg tcaagtcgca ggccatcctt aacgccatcg cactcaatgg
      541 caaccagctg gaccccaccg tcgggctggt gggcatcaac gtgtttgcca acggtatcga
      601 cacgcgcacc gagggcggtg atttcacttt gaatttcccg ttcgactacg ggagcttcgg
      661 gcacgtcacc tacgcagtgg cgatcgacta caacgacacg agcgcgacac gtatcccatc
      721 gacccaactt ggcggggtcg cgctctatga cgtcgaggcg atctcggacg tcaccacggc
      781 caatccgaag tacacgattg acctcaacgc agcgtggagc aacggtccgc tgagcgtcac
      841 gctggatgaa aagatctacg gcccggcctc tgaatacgag aacgacgatg ccgacaacgg
      901 tggcggtgga ccctatcccc cctcacctct ccccagtggc ttcgactact tccgctcgag
      961 catcggcgtc acgccgatca ccaacctgct ggtgtcctac cagttgacga agtggctgaa
     1021 gatctccggc ggtgccaaca atctgttcaa ccgcattccg ccgacgctca acgccacgct
     1081 gctggctcat gaggataact tcttctacgg cgacaaccag ggcgtgcagc attacccgga
     1141 ctggtcgccg ttcggcatca acggtggctt ctacttcgcg aaggccaccg tcacgtggtg
     1201 agcgtgcgat gaatcaccgc tagggatact gcgcgtcggg cactgcgcga gcggggcggc
     1261 gatcgcttgg atcgccgccc tttcttgtcc gttatctcaa ccactcacgg cttgtgcttg
     1321 gcgcccgtct tggcgggcgg ccgcgcgagg atcacttcca cctcgaccat gatgcccggc
     1381 tgagccaacg ccgcgacctg gactgcagag cgggccggca gatttggctg gctcgatgtg
     1441 ccgaagaact gtgtataggc gtccatgaat ccctggaaat ccatgcgccc ttgcttggca
     1501 ggatcgccca ccaggaacgc cgtcatcttg atcacgtcac caaagcccag tcccttctca
     1561 tcgagcgacg ctttcagggt gttgaacaca ctcatcgcct gtgtcctggt atcaccccag
     1621 tagtcgcccg agcccgctgc ggctttggga tccttgggcg aaggcacggt accactgtgg
     1681 aagatcagcg tcgtgcctgc ggatacctcg accgcctgag cgatcggaaa ctggttgccg
     1741 ttcggcaatg gatagcgttt cacgtcatct gcgagcgctg cgaccgccgg ccacaatgtg
     1801 agcaacagcg ctaccttgaa cctggttttc atgatcagac tcccctcttt cagttgagga
     1861 aaccgcggcg ggtgaatgat gcagacatga gaggtcggct ccccttaaac tttcccggca
     1921 gttaagaata cgcgattgtg cgaaccgctt agcgccgcgc ctttggaacg cctgtcatga
     1981 agtcatgccc gtttcagctt cgcaaggcca gacacgctga ccgcccggcg ctgcaggccc
     2041 tgatcgagcg ttcggcgcgc gagcttggcg caggcgacta tcggcccgaa cagatcgagg
     2101 ctgccctgcg cggcgccttc ggcgtggaca cgcagctgat cgatgacgga acctactttg
     2161 tggtggaggc ggaccgaaaa ctggtgggct gcggcggctg gagcaagcgc cggacgctgt
     2221 ttggtgggga tgcgcaccag gagcgcgacg cccaggaact ggaccctaaa accgatgcgg
     2281 ccaagatccg ggcgtttttc gtggacccgg actacgcgcg tcaaggcatc ggccgggcgc
     2341 tactggagcg ctgtgagagt gaagcgcgaa cgctgggctt cagtcacttt gaactgatgg
     2401 gaacgctgcc cggggttcgt ctgtatcagg cgttgggcta cgttggcagc gagcccgtgc
     2461 actacccggt cgcaccgggc gtgacgattg aattcgtccc gatgcaaaag gcctaagtcg
     2521 ggaagaaagc gcgctgcacg aaccagacca gtgcgatcgc tgcgatggcg gccgatcccc
     2581 acggcatgag cgtgcgacga tacagcgtgc cggcccgcac cgcatagacc agcggcatca
     2641 cgctgagcac caccgcgagc tgaccgacct cgatgccgag gttgaaggac accagtgaga
     2701 tcagccttgc gccgtgcggc agtcccatgt cggccagcac cgaggcgaag ccgaatccgt
     2761 gcagcagtcc gaacaggaac gcgatccgcg cgcgcgagtc ggtgacgagc ggaaaaatgt
     2821 tgttgagtgc agcgatgatg atcgaggcgg cgatgaccga ctcggtgaga cgactcggca
     2881 agcgaacgac atccagcgcc gccagcgaca gcgtgatcga gtgcgcgagt gtgaaggcag
     2941 tcaccacctt caggatattg atgaacgccg gtcgcacttc cgacaccggt tcccagtgac
     3001 cgttgcgccg tagcagcacc gccggcaaca gcaggaacag caggtgatcg atgccgctcc
     3061 agatgtgcca gatgccggcc tgcagatatt cggtaaacgc gcgccagcca gaggattcgc
     3121 cgagcgtgaa cgcctgttgc gactgccggc cacccagcac cgccgtctgt gcgtccgcac
     3181 ccgagatcag cgtcagcagg ccgcggtgcg caggatcgac accggccatc aggctatagc
     3241 tgatgcggac ccgacgaatg gcgagcggac actgcgcgct gaagggcacc caggcgtagt
     3301 tgccgtcgac gcgctggttc acctgaattg gctgaaactg caacgcgcag tgtgactttt
     3361 cggtgtcgaa ccccagatga gcctgaatgt actgttgcag cctcggttcg gcggcccgca
     3421 attcacccca ggtcaccctg ccatcgttgt tgctgtccac acccaccgcg agttccgcgt
     3481 cgcgcagcgc cagctcgatc gagccggtca gttcctgacc cgatgcgtgt accgcgagaa
     3541 agccggtgct cgcgacatgc gcactcgcga tcgatgcgag cgaacacagc gtcagtagcg
     3601 cgagtactcg tttcattgca gcgccgcctt caccgcatcg agccgcacgt cctcgagatg
     3661 ttcctgctga atgaactgca gagccggttt cgccgcatcg gactgcctgg cggcctgcgc
     3721 ggcgcgcagc agaatcagga tatccgccgg ctcacgctgg gtcttccagt cctcctgcgc
     3781 ggccgcaagc gcggcgccgg gctgatgctc gacatcgagc aggtagcgtg cctgctcgcg
     3841 gcggtgcacg gcctcgccgc gctgctcttc cacgtcgaac gcgttcccca ggtaggcgcg
     3901 cgcctgttcg ccctgcgcat cgttcagggc ctcgtgagcc agcgcgatcc gcagcagcat
     3961 cggctcctgg gtctcatagc cagcgagcag ttgcagcgtc tcgttggcgc gatggtgttg
     4021 cagcagcagg tccgacagcg ccgcacgcag gtagaagtca tcgggcgcca cctgcaggcc
     4081 ctgacgaaac cagcgctccg cgtcgtcgtc cttaccgagg tgcgaggcca tctcgcccag
     4141 ctccgacagg cgccacgccg tcagctcagg tgttgaaacc tgaaatcgaa gcttttcgag
     4201 cgtgtcgtag gcggactgca gatgcccggt cagggccagc agactctgct gacagagcat
     4261 cgccaccgcc ggctcggagc gcgcggccat gtgcgtacag gaatcgagtg cttcgtcgta
     4321 gcggcccagc acgcgcagca ccgtcgcccg cgtcagccac gcctgggcgt catcgggatt
     4381 gcgcttgagg acctgatcca attccttcag ggaatcggcg aaatcgtgcc ggctctgcaa
     4441 aatcgtggca tgtagcacca gcacgcgcgg cgataccggt gtcttcttca tccatggatc
     4501 gagcgcattt tcggcgtagc ccagaaagcg caggtcaccg ctggcccggg cgcgggtgat
     4561 gtagaactgc gccaacggaa tcgctacatc cacgctccgg cgagccagat cggtcggcga
     4621 ggtagccgcg tggtgaacgc cctgcggcag ctcggccagc aactgggaat cattgccggg
     4681 tatataagga gccgccgaga ccggcggcgc cagaccgatg aacagtgctg ccagaagcaa
     4741 cggcatcatc gtgcgcttca tgcctatctc aagctcgaat cagtgcgagt cgacactgat
     4801 cgggttggtg gtttcgctgg tgtccgtgaa cacgtagcca ccatccaccg aaaacggcga
     4861 ggccgtctcg gagctctgct gcgcgagcgt cagcacctga gccgtgttga cggtcacacc
     4921 gctggagctg ctcataccgc ccgagctcat accgcccgag cttgtcaccg gcgtcgtcgg
     4981 atggctgctg ccgcacgctg ccaggaaggc ggtcgcggct gcggcagctg ccaacagaac
     5041 tagttgacgt ttcatagtga ttctccttgg ttacgttcgc gatgactgga tcgcattagt
     5101 agtcaggaga acccgggacc ggcggagcca cgtacggaaa cgcggtcagg aagtcgctgg
     5161 gctgcgcttc cacgttgtcc gtgtacagaa actgacgtga catatccgag gccggatctg
     5221 gatccttggc gctgccatca tagggctgca gcagcacacc catggcgaca cgcaacgtga
     5281 tatccacgat gtcgtcgatc ggtcggcgac cattgggata gcccgcattg tcgccagcga
     5341 tcacgcccag gtcgttctga gaaccggacg cgaccacagg tgtgttggtg ttcaatcgca
     5401 gcatctcacc gcccacggtc tg
//
LOCUS       DATMRM010000163         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_715737, whole genome shotgun sequence.
ACCESSION   DATMRM010000163 DATMRM010000000
VERSION     DATMRM010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715737"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VNZ06_04820"
     CDS             <1..531
                     /locus_tag="VNZ06_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWW20105.1"
                     /translation="SHAEVRAILAVVRNPIHRTCFQLMYACGLRIGEAAKVAIGDIDN
                     RKGVLRIIGKGNKERCVPLPEPMLVKLRRLWSTHRNPRWLTPDRSGSGPVSKNALWRT
                     FRLAVRAAGIKRHVHPHGLRHSYATRLLEQGTEARVVQILLGHVNIATTAVYLHLTEP
                     TRASLKATLDKLMTDL"
     gene            547..1674
                     /locus_tag="VNZ06_04825"
     CDS             547..1674
                     /locus_tag="VNZ06_04825"
                     /inference="COORDINATES: protein motif:HMM:NF016847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWW20106.1"
                     /translation="MVALSEVFRRFADDYLSQHSLLVLPSHRRAIADILACRTHALGG
                     HLWRCNHCSSEVYSYHSCKNRSCPRCHQDQTERWIAAREAELLACPYFHVTVTVPREL
                     REVLRANQCDGYAALMKATAEAIIELARDRRYVGGTVGVMAVLHTWTQQLVYHPHVHC
                     LVTGGGVSDDGRDWHPARSSFLIPTQPLAVLVRAKMRAALAKRRPDLVLPKAAWQKPW
                     VTHCTAWGDGAEAVLRYLARYVFRVAITERRIVGLDDDGVTIRHKHRASGHWHTTRLN
                     GHEFMRRFLQHVLPKGLHKVRYSGLWHHSRRDHAARARQRLALDHSRVAGPKQEAAEP
                     VASARDCSTAPPSSPPRRCPYCKAGHLQHVRMLYPRQVRGP"
     gene            complement(2323..>2541)
                     /locus_tag="VNZ06_04830"
     CDS             complement(2323..>2541)
                     /locus_tag="VNZ06_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20107.1"
                     /translation="IDIADRNLGRLADAQATGIHQLKAGPVDWITHRNQDGADFGMGE
                     HDRQPLLSRRANAFFWENNAQSRSSVLE"
BASE COUNT          475 a          849 c          739 g          478 t
ORIGIN      
        1 tctcatgccg aggtccgcgc catcttggct gtggtgcgta atccaatcca caggacctgc
       61 tttcaactga tgtatgcctg tgggctgcgc atcggggagg cggccaaggt cgcaatcggc
      121 gatatcgata accgcaaggg agtgctgcgg atcatcggca aaggcaataa ggagcggtgc
      181 gtcccgctcc ccgagccaat gcttgttaag ctgcgccgtc tgtggtcgac ccaccgcaat
      241 ccgcgctggc tcacgcccga tcgcagcggg tccgggccgg ttagcaagaa cgcgctctgg
      301 cgcacattcc gcctggctgt ccgcgccgcc ggcatcaagc gtcacgtcca tccccatgga
      361 ctacgccaca gctacgcaac acggctgctt gagcaaggca ccgaggctcg cgtggtgcag
      421 atcttgctcg gccacgtcaa catcgcaaca acggccgttt atctccatct gaccgagccc
      481 acacgcgcct cgctcaaagc aaccctcgac aaactcatga ccgacctgtg atcgcgacga
      541 ctggtcgtgg tcgcactgtc tgaggtcttc cgccggtttg cggacgacta tctgtcgcag
      601 catagtctgc tggtgctgcc ctcgcaccgg cgtgctatcg ccgacatcct cgcctgccga
      661 acgcacgcgc tcggcgggca cctgtggcgc tgcaatcact gcagcagcga ggtctactcc
      721 tatcattctt gcaagaaccg tagttgccca agatgccatc aagatcagac cgagcgctgg
      781 atcgcggcgc gcgaggccga gttgctggca tgcccctatt tccacgtcac ggtcaccgtg
      841 ccgagggagc tgcgcgaagt attacgcgcc aatcagtgcg acggctacgc cgcgctgatg
      901 aaggcgaccg cggaggccat catcgagctg gcccgtgatc gccgctacgt cggcggcacc
      961 gtcggggtga tggcggtgtt gcacacctgg acgcagcagc tcgtctacca tccgcatgtg
     1021 cattgcctgg tcacgggcgg cggtgtgtcc gatgatggcc gcgactggca tcccgcccgc
     1081 agcagcttcc tgatcccaac gcaaccgctc gcagtccttg ttcgggctaa aatgcgagct
     1141 gctctagcaa aacgccgacc cgatctggtc ctgcccaagg cagcatggca aaagccgtgg
     1201 gtcacccact gcacagcttg gggcgacggc gccgaggccg tgctgcgcta tctggcacgc
     1261 tatgtctttc gtgtggcgat caccgaacgc cgcatcgtcg ggctcgatga cgacggcgtc
     1321 accatccgcc acaagcatcg cgcatcgggg cattggcaca caacgcgcct caacggccat
     1381 gagttcatgc gccgcttcct gcaacacgtg ctaccgaagg gtctgcacaa ggtccgctac
     1441 tctgggctct ggcatcactc tcggcgcgat catgccgcgc gcgcccgtca gcgattggca
     1501 ctcgatcact cgcgcgtcgc agggccgaaa caggaggctg ccgaaccagt cgcgtcagca
     1561 agagattgct caaccgcacc gccgtcctca ccaccacggc gctgcccgta ctgcaaggct
     1621 ggccatctcc agcatgtacg catgctctac cctagacagg tgcggggacc atgatccgat
     1681 gcagtgagcg ccagcgattg tggccgcgcg tgccggacac gccacggatc gctattgccc
     1741 acgccttgcc tttcttgcac gcaccggcac gcatcggttg gaacgctccg caatctcgcc
     1801 ccccaatcac cacatcgatc cctcatcgca ttgccaaaga gccaaaggcg tcacccgaaa
     1861 tcgcctcgac cgtctgagcc tgcgccttaa aattccatag accccgctca ggacccaatt
     1921 ccgcgcccgc gttcagtcca accgagtttc tacgtcggct acgctccgca gaaactcata
     1981 cttcgttgga ctgaacggct tcgccgtagg gggtgtcgtg gatcacggct ggggatatct
     2041 actcgggtgc cgggacagag aggtcctggt gtatgaaccc gaatggagaa caacgatgac
     2101 cccgttgcgt cagcgtatga ttgatgacat gcggcttgct ggctttgccg agaggacccg
     2161 ggacgtttac atccaagcag tccgccggct tgcggcccac tacaggcgct cgcccgatgt
     2221 gctgagcgag gaggaggttc gcacctatct ccttcatctg cgcgatgagc gcggcgtcgc
     2281 gcgtggcacc ttcaagacca atcacggcgg aatccgcttc ctttattcga ggacgctcga
     2341 tcgcgattgg gcattgttct cccaaaaaaa cgcgttcgcg cgccgagaaa gcagcggctg
     2401 ccgatcgtgc tctcccatgc cgaagtccgc gccatcttgg ttgcggtgcg taatccaatc
     2461 cacaggacct gctttcaact gatgtatgcc tgtggcctgc gcatcggcga ggcggccaag
     2521 gttgcgatcg gcgatatcga t
//
LOCUS       DATMRM010000164         5977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1031201, whole genome shotgun sequence.
ACCESSION   DATMRM010000164 DATMRM010000000
VERSION     DATMRM010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031201"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /gene="hflX"
                     /locus_tag="VNZ06_04835"
     CDS             complement(<1..635)
                     /gene="hflX"
                     /locus_tag="VNZ06_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWW20108.1"
                     /translation="MFERPRAGERAVLVRLGLGDAVRPEDLDEFARLARSAGAQPVAT
                     VTGRRQRPDSRYFVGSGKAEEIASAGRSQNAEVILVDHPLSPSQERNLEKLTGVRVID
                     RSGLILDIFAQRARSFEGKLEVELAQLKHLSSRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLAARVRSVTRRLQRLKQQRDTGRSARAQIPVPSVALVGYTNAGKST"
     gene            complement(657..908)
                     /gene="hfq"
                     /locus_tag="VNZ06_04840"
     CDS             complement(657..908)
                     /gene="hfq"
                     /locus_tag="VNZ06_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HWW20109.1"
                     /translation="MAKGQSLQDPFLNALRKEHVPVSIYLVNGIKLQGQIDSFDQFVV
                     LLKNTVSQMVYKHAISTVVPARAVRLAVEEGAAAEPAAD"
     gene            complement(1032..2003)
                     /gene="miaA"
                     /locus_tag="VNZ06_04845"
     CDS             complement(1032..2003)
                     /gene="miaA"
                     /locus_tag="VNZ06_04845"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HWW20110.1"
                     /translation="MAAARPLLVVLTGPTGTGKSELALQLADYLTQRRPLEIVSVDSA
                     QVYRGMDIGTAKPSRAVRARVPHHLIDIRNPCESYSAGDFVRDARIAIDAIHGRGRVP
                     LLVGGTMLYLRALHDGLATLPAASSPMRQTIDAEAVRCGWPAMHAELERVDPEAAAHI
                     ASHDAQRIQRALEVYRLTGIPISAWQRRTHGARDEFQWLRYALLPDSRTELRQRLSLR
                     FQDMLQAGLVEEVRALQARGDLSERHPAMRAVGYRQIWRFCRGDCSLEEASEQAVVAT
                     AQLSKRQMTWLRREQGMITLTAQSHGQAIRVGAEIESRCAHDRPSAR"
     gene            complement(2007..3770)
                     /gene="mutL"
                     /locus_tag="VNZ06_04850"
     CDS             complement(2007..3770)
                     /gene="mutL"
                     /locus_tag="VNZ06_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HWW20111.1"
                     /translation="MPIQVLPANLIDQIAAGEVIERPASVVKELIENAFDASATRIEI
                     DVERAGLGLIRVRDDGSGMDAADLSLAIERHATSKIATLDDLEGIASFGFRGEALPSI
                     ASVARVRLVSRQREAAQANELSVEGGERSDLKPAAHPVGSSVEVRDLFYNVPARRKFV
                     RSEGTEFAHILRQVERLALSEPKVALRLKHNGREVLDLPAAVSSEQIEMRLDRIVGPQ
                     FRSRAIAIEQIAGPMQLTGWLGLPTASRAQADMQYWFVNARAVRDRLLGNAVRLGYRD
                     VLYHGRHAAYVLYLTLDPRLVDVNAHPTKLELRFRDTRAVHDGVFRCIERALAVTRPA
                     QAIPSATALPSPPALAVSTAPTAMPLRLYAGVSATAAFQSYAAAPATARIAELQPGRE
                     LERPLGTAIAQLHGLYILAQTPEGMIVVDTHAAHERVLYEQLKSQYDGSTPASQSLLE
                     PLTVTAAEHEVEALLLAGAQFERVGFELERYTPHSLVVRRVPALLMRSDIVAMLAQLG
                     RELTGEAGSHHLDAAAHRILGSIACRSAIRGQRTMSLGEMDALLRQMEQTDRASQCNH
                     GRPTWLRLTLREIDQLFLRGR"
     gene            complement(3804..5084)
                     /locus_tag="VNZ06_04855"
     CDS             complement(3804..5084)
                     /locus_tag="VNZ06_04855"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWW20112.1"
                     /translation="MYRIHRWAWILGLFAGSPLAFARDAPPARLESIGLQGDERGALL
                     TLSLSAPVAQHLFRLHNPERLVIDLPATTRRAKLPPPSGSGPVVSIRSGRKGEQLRLV
                     VELRKPTAAHLQPSLVAERYRLQIAFGHVLPAAPALAAAPKASVPRSAVASAAAPASA
                     TPAAAIRAAHAPQGERDIIIAVDAGHGGDDPGATGMTGTHEKDVTLAIARALAERIDR
                     EPGMHAALTRNGDYFVALPNRIARARAAHSDMFVSIHADAIRDREVSGASVYILSERG
                     ASSEAARLLADQENSADLKGGISLHEQRPDVRSVLLDLSQNAAIGQSVEAADRVLGAL
                     DRVGAVRKHEVQQAAFVVLKSPDIPSMLVETAYISNPLEERKLRTPVEQERLADAIFS
                     GIDSYFRKFPPEGSLYAHASEHLAGDTGLARGGF"
     gene            complement(5173..5664)
                     /gene="tsaE"
                     /locus_tag="VNZ06_04860"
     CDS             complement(5173..5664)
                     /gene="tsaE"
                     /locus_tag="VNZ06_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWW20113.1"
                     /translation="MSPVWPLPQVQCTEQLGRALARSCPWSDSTARVLYLCGELGAGK
                     TTLASALLHELGVTEPVRSPSYALIELYQAGAHPAVHIDLYRLSAPTELQTLGLRDYL
                     NCQTLLLIEWPERGGSALPSADLRVELTTQPARSASARAESDAGQVWLAAMNPAMMAE
                     VKT"
     gene            complement(5661..>5977)
                     /locus_tag="VNZ06_04865"
     CDS             complement(5661..>5977)
                     /locus_tag="VNZ06_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HWW20114.1"
                     /translation="VLKGAGTLVGVLEASDNTDDANAPDPIYGLCERGNPAMATAGMG
                     DVLTGAIAGILGQCHDPWLAARAGVMAHALAGDDIARDRQRGVLALELAEALNRFVNL
                     RR"
BASE COUNT         1076 a         1952 c         1875 g         1074 t
ORIGIN      
        1 gtcgacttgc cggcgttggt gtagcccacc agcgccaccg acggcaccgg aatctgcgcg
       61 cgtgcactgc ggccggtgtc gcgttgctgc ttgagacgct gcaggcggcg tgtcactgag
      121 cgcacgcgcg cggcaagcag acggcgatcg gtttcgagct gcgtctcgcc aggaccgcgc
      181 aggccgatac cacccttctg gcgctcgagg tgcgtccagc cacggaccag gcgactcgac
      241 agatgcttga gctgggccag ttcaacttcg agtttgcctt cgaagctgcg cgcgcgctgc
      301 gcgaagatat ccagaatcag accgctgcga tcaatcacgc gcacgccagt gagcttttcg
      361 agattgcgct cctggctggg cgacaacgga tgatcgacca gaatgacttc agcgttttgc
      421 gatcgccctg cgctggctat ctcttcggcc ttgccactgc cgacgaagta acgcgagtcg
      481 ggtcgctgtc ggcgtccggt gacggtcgcg accggctgcg caccggctga gcgggcaagg
      541 cgcgcgaatt catcgaggtc ctcgggcctg accgcatcgc caagcccaag tcgcaccagg
      601 acagcgcgct caccggcgcg cgggcgttcg aacatgcaac cttctggatc gagtgatcaa
      661 tcggcggcgg gttcggcggc agcgccctct tcgaccgcga gacgtacagc ccttgccggc
      721 acgacggtgg atatcgcgtg tttatagacc atctggctga cagtgttctt gagcagcacg
      781 acgaactgat caaaagagtc gatctggccc tgaagtttga tgccattgac tagatagatc
      841 gaaacaggca cgtgttcctt gcgcagcgcg ttcagaaacg ggtcttgtaa tgattgacct
      901 ttggccatga gattctcctt atttttcaac tggttttgtt gcttataagt ggtcggcgca
      961 tccgaaagga gtttcggagc accgcccgct acgacaagtc cgtggggagg aagcacaggt
     1021 gtttcattct atcaccgagc gctgggtcgg tcatgcgcgc aacgactctc aatctcggct
     1081 ccaacgcgga ttgcctgccc gtgagactgc gccgtgagcg taatcattcc ctgttctcgc
     1141 cgtaaccaag tcatctgacg cttggagagt tgcgccgttg cgactaccgc ctgctcgctc
     1201 gcctcctcga ggctgcagtc cccccggcaa aatcgccaaa tctgtcgata tcccaccgcc
     1261 cgcatcgccg ggtgacgctc gctcagatcc ccacgcgcct gcaaggcgcg cacttcctcg
     1321 accagacccg cttgcaacat gtcttgaaag cgcaacgaaa ggcgttgccg caattcagta
     1381 cgcgaatcag gcagcaacgc gtagcgcaac cattgaaatt catcgcgtgc gccgtgagta
     1441 cgtcgctgcc atgcactgat tggaatgcct gtcagacggt acacctccag tgcgcgctga
     1501 atacgctgag catcatggct tgcgatatgc gcggcagcct cgggatcgac tcgctccagt
     1561 tcagcatgca tcgccggcca tccgcagcgt acggcctcgg cgtcaattgt ctgacgcatc
     1621 ggggaagatg cggccggaag tgtcgccaat ccgtcgtgca atgcgcgcaa gtaaagcatc
     1681 gtcccgccca ccagcagcgg aactcgaccc cgcccatgga tcgcatcgat cgcgatccgg
     1741 gcatcgcgca cgaagtcgcc ggccgaatag ctttcacacg gattacggat atcgatcaag
     1801 tgatgcggta cccgagcccg cacagcccgc gagggcttcg cagtgccgat gtccatcccg
     1861 cggtagacct gcgccgaatc cacactgacg atctcgagcg gccggcgttg cgtcagatag
     1921 tcggccagtt gcagtgccag ctcactttta ccggtcccgg tgggtccggt cagaaccacg
     1981 agcagcggcc gcgccgcggc catgggctag cgacctcgca gaaacagctg atcgatttcg
     2041 cgcagcgtca ggcgcaacca cgtcggccgg ccgtgattgc attgactggc tcggtcggtc
     2101 tgttccatct ggcgcagcag cgcgtccatc tcgccgagcg acatcgtgcg ctgaccgcga
     2161 atcgctgatc gacaggcgat gctgccaagg atgcgatggg cggcagcgtc gaggtgatgg
     2221 cttcccgcct cgccggtgag ttcgcgtccg agctgcgcca gcattgccac gatatcgctg
     2281 cgcatgagca gcgccggcac cctgcgcacc accaggctgt gtggcgtgta gcgctccagt
     2341 tcgaatccga cacgctcgaa ctgcgcgcct gccagcagca gcgcttcgac ctcgtgctcg
     2401 gccgccgtga cggtcagcgg ctcgagcagc gactgcgaag ccggcgtgct gccgtcgtat
     2461 tgcgacttga gctgttcata cagcacgcgt tcgtgcgcgg catgcgtgtc taccacgatc
     2521 atgccttccg gtgtctgcgc caggatgtac aggccgtgaa gctgagcgat cgcagttccg
     2581 agcggacgct ccagttcccg cccaggctgc agctccgcaa tgcgcgctgt ggccggggct
     2641 gcggcgtagc tttgaaatgc agccgtggcg ctgacgcctg cgtacaggcg cagtggcatg
     2701 gccgtcggcg cagtgctcac ggcgagcgcc ggcggcgatg gcagcgccgt cgccgagggg
     2761 atggcctgcg ccggtcgggt gaccgcgagc gctcgctcga tgcaacgaaa aaccccatcg
     2821 tgtacggcgc gcgtatcgcg aaagcgcaat tcgagcttgg tcggatgcgc attgacatcg
     2881 accagccgcg gatcgagcgt caggtagagc acgtaggccg catgacgccc atggtagagc
     2941 acgtcgcggt aacccaggcg cacagcgttg cccaaaagtc ggtcacgcac agcgcgggca
     3001 ttcacgaacc agtactgcat gtcggcctgt gcgcgcgagg cggtcggaag gccaagccat
     3061 ccggtcagct gcatcggtcc ggcgatttgt tcgatcgcga tcgcgcgact gcgaaactgc
     3121 ggtccgacaa tccgatccaa ccgcatctcg atctgctccg acgatactgc agcgggcaga
     3181 tccagcacct cgcggccgtt gtgcttcagc cgcagcgcca ccttgggctc ggacagggcc
     3241 agacgctcga cctggcgcag gatgtgagca aactcggtgc cctcactgcg gacgaatttg
     3301 cgacgcgccg gcacgttgta gaacagatcg cgcacttcca cgctgctgcc cacgggatgg
     3361 gcggccggct tgaggtccga gcgctcgccg ccttcaacac tgagctcgtt tgcttgcgca
     3421 gcctcgcgct ggcgcgacac caggcgcaca cgcgccaccg atgcgatcga aggcagcgct
     3481 tcgccgcgaa agccgaagct ggcgataccc tccagatcat ccagcgtcgc aattttgctg
     3541 gtcgcgtggc gctcgattgc cagtgacaga tccgcagcgt ccataccgct gccgtcgtct
     3601 cggacgcgga tcaatcccag gccggcgcgc tcgacgtcga tctcgatgcg cgttgcgctg
     3661 gcatcgaatg cgttctcgat cagttctttg accaccgacg cggggcgttc gatgacttcg
     3721 ccagcggcga tctgatcgat caggttggcc ggaagaactt gtatcggcat gggcgcttag
     3781 gctcaaagcg cacagtgtac gagtcagaag cctccgcgcg cgaggcccgt atcgccggcg
     3841 agatgctcgg acgcgtgggc gtacaggctg ccttccggcg ggaacttgcg gaagtagctg
     3901 tcgataccgc tgaagatcgc atcggccagc cgttcctgct caaccggcgt gcgcagtttt
     3961 cgctcctcga gcggattgga aatgtaggca gtttcaacca gcatcgaggg gatatcgggc
     4021 gacttgagca ccacgaaagc ggcctgctgt acctcgtgtt ttcgcactgc tccaacccga
     4081 tcgagcgcac cgagcacacg atcggcggcc tccacactct gtccgatcgc cgcgttctgc
     4141 gacagatcca gcagcaccga gcgcacatcg gggcgctgct cgtgaagcga gataccacct
     4201 ttgagatcgg ccgaattctc ctggtcagcg agcagcctcg ccgcctcgct cgaggcaccg
     4261 cgctcggaca ggatatagac cgaggcaccc gacacctcgc gatcgcggat cgcatcggca
     4321 tggatcgaga caaacatgtc ggaatgcgcc gctcgggcgc gcgcgatgcg gttcggcaat
     4381 gcgacgaagt agtcgccgtt gcgtgtcagg gccgcatgca ttccaggctc ccgatcgatg
     4441 cgctcggcca gcgcccgcgc gatcgcaagc gtcacatcct tctcgtgcgt gccggtcata
     4501 ccggtcgcgc ccgggtcgtc gccaccgtgg ccggcgtcga ccgcaatgat gatgtcgcgc
     4561 tcaccctgcg gggcatgcgc tgccctgatg gctgcagccg gcgtcgcact ggctggcgcc
     4621 gcggccgagg caacggcgct gcgcgggacg gaggccttag gtgccgcggc caatgcgggc
     4681 gctgccggca gtacatggcc gaacgcaatc tgcagtcggt agcgctccgc gacgaggctg
     4741 ggttgcagat gagcggcggt gggcttgcgc agttccacca ccagtctcaa ttgctcgccc
     4801 ttcctacccg agcgaatcga caccacagga cccgaacccg acggcggcgg caattttgcg
     4861 cgacgcgtgg tcgctggcag atcgatgacc agtcgttcag ggttgtgcag acggaagagg
     4921 tgctgtgcga ccggggctga cagcgagagc gtcagcagtg ctccgcgctc atcgccttga
     4981 aggccgatcg actccaatcg agcgggcggt gcatcgcgcg cgaacgcaag aggacttccg
     5041 gcgaacagcc cgagtatcca agcccagcga tgaattcggt acaagcggcg cgctccccta
     5101 cgccaaggcc tgcgggactg tattccgatc ttctattaat ttcaatgatt attaatcaga
     5161 tacgcgcgat atctaagtct taacctcagc catcatcgct ggattcatcg cagccagcca
     5221 cacctgaccg gcatcgctct cggcccgggc gctggcactt cgcgccggct gcgtcgtgag
     5281 ctccacccga agatccgccg atggcagggc cgaaccgccg cgctcgggcc attcgatcag
     5341 caacagggtc tgacagttca gatagtcgcg tagccccagg gtctgaagct ccgtcggggc
     5401 actgagtcga tacaggtcga tgtgcaccgc cgggtgcgct ccggcctgat acagctcgat
     5461 cagggcgtag ctcggactgc gaaccggttc ggtcacgccg agctcatgca acagtgccga
     5521 ggccagcgtg gtcttgccag ctcccagctc accgcacagg tacagcacac gcgccgtgct
     5581 gtcgctccag ggacagctgc gcgccagcgc tctgccgagc tgctcggtgc attgcacctg
     5641 cggcagcggc cataccgggc tcatcgacgc agattgacga agcgattgag ggcttccgcc
     5701 aattcaagtg ccaacacgcc gcgctggcga tcgcgtgcga tgtcatcgcc cgccaatgcg
     5761 tgcgccatga cgcccgcacg cgccgcaagc caggggtcat gacactgtcc gagaatgccg
     5821 gcgatcgcgc cggtcagcac gtcccccatt ccggcggtgg ccatggctgg attgccgcgc
     5881 tcgcacagtc catagatcgg atcgggcgca ttggcatcgt cggtgttgtc ggaggcttcc
     5941 agcactccga ccagtgtgcc ggcgcctttc agcacga
//
LOCUS       DATMRM010000165         4019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1339356, whole genome shotgun sequence.
ACCESSION   DATMRM010000165 DATMRM010000000
VERSION     DATMRM010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339356"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VNZ06_04870"
     CDS             complement(<1..356)
                     /locus_tag="VNZ06_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWW20115.1"
                     /translation="MPNAHTRTTTIELSEAERTELSSMARSRSLPAALALRARIGLAC
                     EGDDRASTAVALKLGINRGTVNKWRGRYARQRIAGLYDELRPGRPRSVDDEPVAELIN
                     TTLHSKPADGGTHWSTR"
     gene            complement(406..1362)
                     /locus_tag="VNZ06_04875"
     CDS             complement(406..1362)
                     /locus_tag="VNZ06_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20116.1"
                     /translation="MIRRRWSLALACVAVAASAIAQNGFASNILFIGNSFTFGYGSPV
                     RFYRADSVTDLNNEGIGGVPALFKAFTTEAGLNYDVYLETHPGIGIDWHLQNKRDVLT
                     QRGWDAVVMHGFSMLDRTKPGDPATLISSARQMAELLRSKNPAVELRLTETWPRADQT
                     YDSKGAYFGKSIDVMAQDLHQGYAQAAAAIPGKKTLVASVGDAWMRAIRTGVADSNPY
                     DGIDANKLDLWTFDGYHASTYGYYLEALVVFGTITGQDPRTLGGKECSGFELGLSPPQ
                     VEALEQVAYDQLVADGTVKAGNAAASASGPAARCAAASVLSH"
     gene            1731..3764
                     /locus_tag="VNZ06_04880"
     CDS             1731..3764
                     /locus_tag="VNZ06_04880"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWW20117.1"
                     /translation="MRHVFAALGLMLAAGSVWAGQAPAFTSEQLSAYPLNNWLTNGGT
                     IFNQRYSPLNEINTGNVAKLKADWRVHLNGSGVGPPYSGEAQPIVYDGVIYIPTGADD
                     VFALDVESGKQLWVYQSGLDKKVSTVCCGWTSRGVGLGDGKVFLGRLDGKLVALDQRT
                     GKEVWSVQAERWEDGFTITSAPLYYDGMVITGFAGSEFASRGRVRAFSAKDGKLLWTF
                     YTVPGPGELGHDTWPQDGGAWEHGGATVWQTPAVDPKRGLLYFSTGNPGPDFNGSGRK
                     GDNLFTVSIVALEAKTGKYRWHFQQVHHDLWDYDSPSPVVLFDIDLNGKHREAIAEIG
                     KTGWVYILDRVTGKPLVGIDEQAVPQDARQQTAATQPFPVGDPVVPHEIDIPPAGFRL
                     VNDGKIFTPYWDTMIVAKPAPLGGTNWPPSSVDPRQGYMFVCSNDGMGRFKVAEAKNP
                     KSGSIYLGGLFGYADIPMTGIFAAVDLRTNKIVWHQRWKDTCYSGSAATAGDLVFTGR
                     NDGRFVALDSRNGDVLWEYQTGAGVNATASLFQYKGHEKVVVYSAGNMFGGTPPGDSV
                     YLFSLDGTLGPQAPPAPANTKVATVSAKEADTHNGSQVFAHNCVACHGEEGKGGHGGG
                     PDITAMTSPDVVATAVTNGKNKMPPFSQALSPNDIRDVSVFVAAELGKDSVKH"
     gene            complement(3738..>4019)
                     /locus_tag="VNZ06_04885"
     CDS             complement(3738..>4019)
                     /locus_tag="VNZ06_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWW20118.1"
                     /translation="VRDVDLIRHAYVLPASQQRGIGSALLRFLCGRSSRPILVDTWAV
                     ATWAIDFYRRHGFTLVPPDQTPALLRSYWTISQRQLETSVVLVFDAIFA"
BASE COUNT          778 a         1201 c         1266 g          774 t
ORIGIN      
        1 cgcgtactcc aatgcgtacc cccatcggcc ggcttgctat gcaatgtcgt gttgatcagt
       61 tcggccacgg gctcgtcgtc cacgctgcgc ggtcggcccg gccgcagttc atcgtacaaa
      121 ccagcaatgc gctgacgcgc atagcggccg cgccatttgt tcaccgtgcc gcggttgatc
      181 ccaagcttca aggcaaccgc ggtgctggcc ctgtcatccc cttcgcacgc cagccctatg
      241 cgtgctctca atgcgagcgc tgccggcagc gagcgcgatc tggccatcga gctcagttcc
      301 gttcgctccg cttcgctgag ctcgatcgtg gtcgtgcgtg tgtgggcgtt gggcatgagg
      361 cacctcctag gatgcctcaa accctgcact tcttgtgcct acgaattaat gggacaggac
      421 actagcggcc gcgcagcgcg ccgcaggtcc ggatgcggaa gccgcggcgt ttcctgcctt
      481 gacggtgcca tcggcgacga gctgatcata agcgacctgc tcgagggctt cgacctgcgg
      541 cggggacaat cccagttcga agcccgagca ttccttgcca cccagcgtac gagggtcctg
      601 accggtaatc gtgccaaaca cgaccagggc ttcgaggtaa tagccgtagg tgctggcgtg
      661 atagccgtcg aaggtccaca gatccagctt gttggcatcg atgccgtcat agggattgga
      721 gtccgcgacg ccggtgcgga tggcacgcat ccacgcatcg ccaaccgagg ccaccagcgt
      781 cttcttcccg gggattgcag ccgccgcttg cgcatacccc tggtgcagat cctgcgccat
      841 gacatcgatg gacttgccga agtaggcgcc ctttgagtcg taggtctgat ccgcgcgcgg
      901 ccaggtttcg gtcagacgca gctcgaccgc ggggttcttg gagcgcagca gctcggccat
      961 ctgcctggcg ctgctgatga gcgtcgcagg atcacctggc ttggtccggt ccagcatgct
     1021 gaaaccgtgc atcacgacag catcccagcc gcgctgagtc aggacatccc gtttgttctg
     1081 cagatgccag tcgatgccga tgcccggatg agtttccaga tagacgtcgt agttgagacc
     1141 ggcttccgtg gtaaacgcct tgaacagcgc gggcacgcct ccgatacctt cgttgttgag
     1201 gtcggtcacg ctgtctgcac gataaaagcg caccggcgaa ccgtacccga aggtaaaact
     1261 gttaccgatg aaaaggatgt tgctggcaaa gccattttgc gcaatcgcgg aagcggccac
     1321 ggcaacgcag gccagcgcaa gcgaccatcg acgacgtatc atgtaacgat actagtcaag
     1381 agcgggggta ctcggcaatg ttggtaccga gttcgccaga gtccacgcgg atcatggaca
     1441 gcctcaagcg actcatggaa tcgcgaggca tcacctatgc agagctggcc cgacggatcc
     1501 gctcgagctc cgatacgggc aggtgcgaat cggcccgggc ccttgagccg ctggcgatac
     1561 ggtgatgtct gcccgggttt ctcaacaatc ggtggaatca cgccttcaag ctggtgtacg
     1621 cctggcgcgt tcgctcagaa catggtttcc ggttaacatt ctgtctactc agatgaaagg
     1681 ctagtaacgc ccgtccaaga cgggcgttgc tcgatagggg gccggtcgac atgcgtcatg
     1741 tgttcgcagc gcttgggttg atgctggcag caggcagcgt ttgggcgggc caggcgcccg
     1801 catttaccag cgagcaactc tccgcctacc cgctcaacaa ctggctcacc aacggtggca
     1861 cgatcttcaa tcagcgctac tcgccgctga acgagatcaa cacgggcaac gtcgccaagc
     1921 tcaaagccga ctggcgcgtg catctgaatg gttcgggtgt gggcccgccc tattcgggcg
     1981 aggcgcaacc gatcgtctac gacggggtca tctacattcc aacgggtgcg gatgatgtct
     2041 ttgcactcga tgtggagagc ggcaagcaac tctgggtata ccagtccgga ctcgacaaga
     2101 aggtgagcac ggtgtgttgc ggctggacca gccgcggcgt cggcctgggc gacggcaagg
     2161 tgtttctggg acggttggat ggcaagctgg tggcgctcga tcagcgcacc ggcaaggagg
     2221 tctggtcggt acaggcagag cgctgggaag atggtttcac gatcaccagc gcaccgctgt
     2281 actacgacgg tatggtgatc accggtttcg ccggctcgga attcgcgtcg cgtggccggg
     2341 tgcgcgcctt cagtgcgaaa gacggcaaat tgctgtggac gttctatacc gttccagggc
     2401 ctggtgagtt gggtcacgat acctggccgc aggacggcgg cgcctgggag catggcggtg
     2461 cgaccgtctg gcagacgccc gcggtcgatc cgaaacgcgg gctgctgtac ttctcgactg
     2521 gcaatcctgg acctgatttc aacggcagcg ggcgcaaggg cgacaatctg ttcacggttt
     2581 ccatcgtcgc gctggaagcc aaaaccggca aataccgctg gcactttcag caggtgcatc
     2641 acgacctgtg ggattacgac tcacccagtc cggtggtgtt gttcgatatc gatctgaacg
     2701 gcaagcatcg tgaggcgatc gcggaaatcg gcaagaccgg ttgggtctac attctggatc
     2761 gcgtcacagg caagccgctg gtcggcatcg atgagcaagc tgttccgcag gatgcgcgcc
     2821 agcagaccgc cgccacgcag ccgttccccg tcggggatcc ggtggtgccg catgagatcg
     2881 acattccgcc ggctggcttc aggctcgtga acgacggcaa aatcttcacg ccctattggg
     2941 acaccatgat cgttgccaag ccggcgccac tgggcggcac caactggcca ccgagttcgg
     3001 tcgatccacg ccaggggtac atgttcgtct gctcgaacga cggcatggga cgtttcaagg
     3061 ttgcggaagc caagaatccg aagagcggct ccatctatct cggtggcttg ttcggctatg
     3121 cggatatacc gatgaccggc attttcgcgg cggtcgattt gcgcaccaac aagatcgtct
     3181 ggcaccagcg ctggaaggac acctgctaca gcggttccgc cgcgacggcc ggtgatctgg
     3241 tgttcaccgg ccgcaatgat ggtcgattcg tggcacttga ctcacgcaat ggcgatgttc
     3301 tctgggaata tcagaccggt gcaggcgtca acgccacagc cagcttgttt cagtacaaag
     3361 gtcacgagaa ggtggtcgtc tactccgccg gcaatatgtt tggcggcact ccgccgggcg
     3421 acagtgtgta cctgttctcg ctcgatggta cgctcggacc gcaggctccg ccggcgccgg
     3481 caaacacgaa agttgcgacg gtgtcagcga aggaggcgga cacccacaat ggttcgcaag
     3541 tgttcgcgca caactgcgtg gcgtgtcatg gcgaggaggg caaaggcggt catggcggcg
     3601 gcccggacat tactgcgatg acctcacccg acgtagtcgc caccgccgtg acgaacggca
     3661 agaacaaaat gccgccgttc tcgcaggcgc tgtcgccgaa tgatatccgt gatgtgtcgg
     3721 tgttcgtcgc cgccgagcta ggcaaagata gcgtcaaaca ctaataccac cgaggtttca
     3781 agctggcgct gcgagatggt ccagtacgat cgaagcaatg ccggtgtctg atcgggcggt
     3841 accagtgtga aaccgtgtcg gcggtagaag tcgatggccc aggttgccac cgcccaggtg
     3901 tccaccagga taggccgcga actgcgtccg cacagaaacc tcagcagtgc gctgccgatg
     3961 ccgcgctgct gactggctgg taatacgtag gcgtgccgaa tcagatcgac atcacgcac
//
LOCUS       DATMRM010000166         7062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3809704, whole genome shotgun sequence.
ACCESSION   DATMRM010000166 DATMRM010000000
VERSION     DATMRM010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3809704"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VNZ06_04890"
     CDS             complement(<1..361)
                     /locus_tag="VNZ06_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWW20119.1"
                     /translation="MTPRHVRQIFEHLRAANPSPQSELAFSSPFELLIAVILSAQATD
                     KGVNVATAKLFPVAATPEAILKLGLTGLKRYIKTIGLYNAKARNILAACRILVEKYGG
                     EVPREREQLESLPGVGRK"
     gene            complement(358..918)
                     /locus_tag="VNZ06_04895"
     CDS             complement(358..918)
                     /locus_tag="VNZ06_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HWW20120.1"
                     /translation="MSEHQNDVNTIDALLPQTQCTRCGFEGCRPYAQALADGSTGINR
                     CPPGGAPGIAALASLLGREVLPLDPSCGSEEDPGVAAIDARECIGCARCLPVCPVDSI
                     IGAHRYLHTVLESACTGCELCIDACPVDCIEMRPRSPGEPAPSRLENRSRYQAHCARA
                     ERETQDKARLLAERKRTAHSLAVRSR"
     gene            complement(915..1520)
                     /locus_tag="VNZ06_04900"
     CDS             complement(915..1520)
                     /locus_tag="VNZ06_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20121.1"
                     /translation="MRRSIRTLITTAILISLSACVSTSIVEHWRDPGFAGPALHKVLV
                     VSVQRDQGRRRLWEEAMVAALAKHAVQAEASYQVFPDQAPSPEKLTAMATRDAFDGVV
                     ATHFVRAGQHVTAYGGWGPWDCCWRPWGWGAWGPGYVESDTLTDYQTDVYTIDASGGK
                     LIWTAVTRSVDPSSAKSVTEGISRALVPQLTKEGILTGTHS"
     gene            1554..2198
                     /locus_tag="VNZ06_04905"
     CDS             1554..2198
                     /locus_tag="VNZ06_04905"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWW20122.1"
                     /translation="MFPKVTPVVRALLYVNFIVYAAQRLTGYGDALLQYFALWPLGTP
                     AYGQAPFEPWQLVTYAFLHDPANLMHIASNMFALWMFGPAAEWVLGSRRFGFYYLLCV
                     IGAALAELLIVPAMYAQGGGVTIGASGGVMGLLVLFGLVFPRQRIFLYFAIPMPAWIF
                     VTLYGVFELWLGVFGSSDSVAHFGHLGGMATGVVLFLFWRNYIRGRVAGPARPK"
     gene            complement(2191..4212)
                     /gene="metG"
                     /locus_tag="VNZ06_04910"
     CDS             complement(2191..4212)
                     /gene="metG"
                     /locus_tag="VNZ06_04910"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWW20123.1"
                     /translation="MPRKILVTHALPYANGPLHVGHIIESVQTDVWVRLQKMRGNDCI
                     FCCAEDTHGTPIMIRAQQEGITPQQLVARSAQEHQRDYAGFLIGFDQYHSTDAPESRR
                     FTYEIYSRVRDAGYIDRRSVRQAYDETAGMFLPDRFVRGTCPNCKSPDQYGDACEVCG
                     TTYTPADLIDPISTVSQTKPVWRNSEHLFFKLNAFDKMLREYVMQASPDSAVRAKLRE
                     WLDAGLQDWDISRDAPYFGFEIPDAPGKYFYVWWDATIGYIGSFEAWRARHGASFDEY
                     WKSETATELYHFIGKDISYFHTLFWPAVLQGAGLRRPTQVLAHGFLTINGQKMSKSRG
                     TFITAQRYLERLPPEPLRYYFAAKLAGNIEDIDFSLDDFVARTNSDLIGKFVNIASRC
                     AVFIERGDGKLAPQLPDPALYQQFTAAGERIAELYETREYGTAIREIMELADRANRYI
                     DQQKPWALAKEPGREPQVRAIATQGLNLFRVLMSYLAPVLPRIAQSAGRWLGADFTRW
                     GAIDQPLLGSPLPAYQPLATRLDAASVATLIDKPSDAPAASPAPASAPAATAKAAAPI
                     SIDDFAKVDLRVARVLLAQAVEGSSKLLNLTLDLGSEHRTVFSGIRGSYQPEQLTGRL
                     VVMVANLAPRKMRFGVSQGMVLCASTDDDSAGIFLLDADSGAQPGMKVT"
     gene            complement(4262..5323)
                     /locus_tag="VNZ06_04915"
     CDS             complement(4262..5323)
                     /locus_tag="VNZ06_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HWW20124.1"
                     /translation="MAQIDSLLEEILKRRGSDLHFIAGDPPRIRLHGDLSPLRAEPTA
                     PEACKAAIYEIMPRKALERFESKDGADFAYTLEGKGRFRVNVMRQLNGMGAVFRAIPS
                     QALTLDQLNMPTAIRQLCHAVSGLILVTGKTGSGKSTSLAAMIDDINKRVKGHILTVE
                     DPIEFVHPRRNCLVSQREIGVHAASFGAALLSALREDPDVILVGELRDLETMSMAVTA
                     AEMGILVMGTLHTNGAAPTVDRMVNAFPSDKQLHVRTMLSTSLRGVISQQLLKKADGT
                     GRVAALEILINTPASANLIRQGKLDQLENVMQTGGQMGMRTMDAAIQQLFDSGQVSGA
                     EAYKKSINKTRFEQHKAEH"
     gene            complement(5327..6439)
                     /locus_tag="VNZ06_04920"
     CDS             complement(5327..6439)
                     /locus_tag="VNZ06_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HWW20125.1"
                     /translation="MARVDAFLKLGTQQGCSDIHLAVGVPPMLRMHGDLVPIKFRDLR
                     DTELESYVTEILTPSQQEHFLRGNDLDFSYVSAEGGRFRVNVYRKDTGVGAAFRAIPT
                     EVPNLAQLGLPPIVTKLCDYHQGMILVTGSTGTGKSTTLAAMIDLLNSTRPVNIISLE
                     DPIEFVHRSKSSQVIQRELGTHIPSFAEGVRAAMREDPDVILVGELRDAETIRMAMTA
                     AETGHLVLGTLHTTSAVKTIDRMIDALPAEEREQTKSFLSQSLLAVVTQILVKTADGR
                     GRRAVVEVMVTTKAIGKLISTDQTHQIPSQLQMGKEFGMQMFDQALLQSLEKKEIDPD
                     DAVVYASDKRLFQKFVTDPTLIPRELPAAAMSQTGS"
     gene            complement(6587..>7062)
                     /locus_tag="VNZ06_04925"
     CDS             complement(6587..>7062)
                     /locus_tag="VNZ06_04925"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWW20126.1"
                     /translation="DISTGMTVAHQVGIVHRDLKPANVLINDDALVKIVDFGVAAAQR
                     EGDTQLTRTGYVIGSPKYMAPEQILGRKVDERADIYALGVMMYEMLTGVPPYSRGDHM
                     AVMYQHVQGKARAPQEINPTLSANLAEVVVKAMAVDKAKRFQTMEELKGALERFL"
BASE COUNT         1224 a         2171 c         2234 g         1433 t
ORIGIN      
        1 tcttgcgtcc gacacccggc aacgactcga gttgttcgcg ctcgcgcggt acctcgccgc
       61 cgtacttctc caccagaatc ctgcaggcgg caaggatgtt cctggccttg gcgttgtaca
      121 gcccgatggt cttgatgtag cgcttgaggc cggtgagccc gagtttgagg atcgcttcgg
      181 gcgtggcggc caccggaaac agcttggcgg tcgcaacgtt gacgcctttg tcggtcgcct
      241 gagcggatag gatcactgcg atcagcagct caaacgggga gctgaacgcc agctcagatt
      301 gaggcgacgg attggctgcg cgcagatgtt cgaatatctg tcgtacgtgg cgtggcgtca
      361 tcgcgagcgc accgcgagcg aatgtgcggt gcgcttgcgc tcagccagca atcgggcctt
      421 gtcctgggtc tcgcgctcgg cccgggcgca gtgtgcctgg tagcggctgc ggttctccag
      481 ccggctcggt gccggttcac ctgggctgcg cggccgcatc tcaatacagt cgaccgggca
      541 ggcgtcgata caaagctcgc acccggtaca ggcagactcg agcaccgtgt gcagatatcg
      601 gtgcgcaccg atgatcgaat ccaccgggca cacgggcagg cagcgcgcac agccgatgca
      661 ttccctcgca tcgatggccg cgacgcccgg atcctcctcg cttccgcagg acggatcgag
      721 cggcagcacc tcccgcccca gcagactcgc gagggctgcg atcccgggcg cgccgcctgg
      781 cgggcagcgg ttgatgccag tgctgccgtc ggccagcgcc tgtgcgtacg ggcggcagcc
      841 ttcgaagccg caccgggtgc actgagtctg tggcagcagg gcatcgatgg tgttaacgtc
      901 gttctggtgt tcgctcaact gtgtgtgcct gtcagaattc cctctttggt caactgcggc
      961 accagcgcgc gagaaatccc ttccgtcacg ctcttggcgg aactcggatc gacgctgcgg
     1021 gtcacggcag tccatattag tttgccgcca gacgcatcga tcgtatagac atcggtttga
     1081 tagtcagtca gcgtgtccga ttccacgtag cccgggcccc atgcgcccca gccccacggc
     1141 cgccagcagc agtcccatgg gccccaaccg ccgtaggccg tgacgtgctg cccggcgcga
     1201 acgaagtgcg tcgcgacgac cccatcgaac gcatcgcgcg tggccattgc ggtcagcttt
     1261 tctggggagg gggcctgatc ggggaatacc tgataggacg cttccgcctg aacggcatgc
     1321 ttggcgagcg cggccaccat cgcctcttcc cagaggcgtc gtcgcccctg atcgcgttgg
     1381 acgctgacga ccagtacctt gtgcagagcg ggtccggcaa agccgggatc cctccagtgc
     1441 tcgacaatgc tggtgctgac acaggcgctc agggaaataa ggatcgcggt ggtgatcagg
     1501 gttcggatgg aacgacgcat ggcgagcctc gcaacagtgg caaaatgcac tgaatgtttc
     1561 ccaaggtaac acccgttgtc cgggcgctcc tatacgtcaa cttcatcgtc tatgcagcgc
     1621 aaaggttgac aggatacggc gacgctctgc tgcagtattt tgccctatgg ccgctgggga
     1681 cccctgccta cgggcaggcg ccctttgagc cctggcaact ggtcacctac gcttttctgc
     1741 acgatccggc caacctgatg cacatcgcga gcaacatgtt cgccctgtgg atgttcggtc
     1801 ctgccgctga gtgggtgctg ggttcgcggc gatttggttt ttactacctg ctgtgtgtga
     1861 tcggagcagc gctggcggag ctgctgatcg ttccggcgat gtatgcgcag ggcggcggcg
     1921 tgacgatcgg cgcctcgggt ggagtcatgg ggctgctggt gctgttcgga ctggtgtttc
     1981 cccggcagcg tatctttctg tacttcgcca ttccgatgcc tgcctggatc ttcgtgacac
     2041 tgtatggagt gttcgaactg tggctgggcg tcttcggcag cagtgacagc gtggcccatt
     2101 ttggccatct gggcgggatg gccaccgggg tggtgctgtt cctgttctgg cgcaactata
     2161 ttcgcggccg cgtggccggg cccgcccggc ctaagtgact ttcattcctg gctgggcgcc
     2221 cgaatcggca tccagcagga agatgcctgc cgagtcatcg tcggtgctcg cacacagcac
     2281 catgccttgc gacactccga agcgcatctt gcgcggcgcg agattcgcga ccatgaccac
     2341 cagccgaccg gtcaattgct cgggctggta gctgccgcga atcccggaga agacagtgcg
     2401 gtgttcactt cccagatcga gcgtcagatt cagcagcttg ctcgatcctt cgacggcctg
     2461 cgccagcagc acacgcgcca cgcgcagatc cactttggcg aagtcatcga tgctgatggg
     2521 ggctgcggcc ttggcagtcg cggcaggtgc cgaggctggg gcgggcgatg ccgcaggcgc
     2581 atcggacggc ttgtcaatca gcgtcgccac gctcgcggca tccagtcgcg tggcgagcgg
     2641 ctgatacgct ggcagaggac tgccgagtag cggctgatcg atggcgcccc agcgggtgaa
     2701 atccgcaccc agccagcgcc cggccgactg cgcaatccgc ggcagcaccg gcgccagata
     2761 gctcatcaga actcgaaaca gattcagccc ctgcgtggcg atcgcccgca cttgcggctc
     2821 gcgcccgggc tccttggcga gcgcccacgg tttttgctga tcgatatagc gattggcgcg
     2881 gtcggccagt tccatgattt cgcggatcgc cgtgccgtat tcgcgcgttt cgtaaagctc
     2941 ggcgatgcgc tcgcccgctg cagtgaactg ctgatagagc gccgggtcag gcagttgcgg
     3001 cgcgagcttg ccgtcgccgc gttcgatgaa cacagcgcag cggctggcaa tgttgacgaa
     3061 ctttccgatc agatcggaat tggtgcgtgc gacgaagtca tcgaggctga agtcgatgtc
     3121 ttcgatgttg ccggcgagtt tcgccgcgaa gtaatagcgc agcggctcag gcggcaggcg
     3181 ctccagatag cgctgtgcgg tgatgaaggt gccgcgcgac ttggacatct tctggccgtt
     3241 gatggtcagg aatccgtgtg ccagcacctg tgtgggccgg cgcaggccag ccccctgcag
     3301 caccgccggc cagaacagcg tatgaaagta gctgatgtcc ttgccgatga aatgatagag
     3361 ctcggtggcc gtttcggact tccaatactc gtcgaacgaa gcgccgtgcc tggcgcgcca
     3421 cgcctcgaag ctgccgatgt agccgatggt ggcatcccac cacacgtaaa agtacttgcc
     3481 cggtgcgtcc ggaatctcga acccgaagta gggcgcatcg cgcgagatgt cccagtcctg
     3541 cagccccgca tccagccact cgcgcagttt cgcgcgcacg gcactgtccg ggctcgcctg
     3601 catcacgtat tcgcgcaaca tcttgtcgaa cgcgttcagc ttgaaaaaca ggtgttccga
     3661 gttgcgccac acgggcttgg tctgactgac cgtggagatc gggtcgatca ggtcggcagg
     3721 tgtgtaggtc gtaccgcata cctcgcacgc gtccccatat tggtcgggcg acttgcagtt
     3781 cgggcaggtg ccgcgcacga accgatccgg caggaacatc ccggcggtct catcgtaagc
     3841 ctgccgcacc gagcgccggt cgatgtaacc cgcatcacgc acccggctat agatctcgta
     3901 ggtgaagcgg cggctctcgg gcgcatcggt agagtggtac tggtcgaatc cgatcagaaa
     3961 cccggcgtag tcacgctgat gttcctgcgc actgcgcgcg accagctgct gcggtgtgat
     4021 gccttcctgc tgggcccgga tcatgatcgg tgtgccgtgg gtgtcctcgg cgcagcagaa
     4081 aatgcagtca ttgccgcgca ttttctgcag tcgcacccag acgtcggtct gcactgactc
     4141 gatgatgtga cccacatgca gcgggccatt ggcatagggc agggcgtgag taacgagaat
     4201 ttttcgcggc atgcccgaca ttattgcatg gccgatacgc cgcggctgcc ggccgggaag
     4261 gctagtgttc ggctttgtgc tgttcgaagc gggtcttgtt gatcgacttc ttgtaggcct
     4321 cggccccgct gacctgcccc gaatcaaaca gctgttggat cgcggcatcc atggttcgca
     4381 ttcccatctg accgccggtc tgcatgacgt tctcgagctg gtcgagcttt ccttgacgga
     4441 tcaggttggc ggatgcgggc gtattgatca ggatctccag cgccgcgacg cgccctgtgc
     4501 cgtcggcctt cttgagcagt tgctgcgaaa tcacgccgcg cagcgacgtg gacagcatgg
     4561 tccgcacgtg cagctgcttg tcagacggaa atgcgttgac cattcgatcc acggtcggtg
     4621 cagcgccatt ggtgtgcagc gtacccatca ccagaatgcc catctcggcg gccgtgaccg
     4681 ccatgctcat ggtctccagg tcgcgcagct caccgaccag gatgacatcg ggatcctcac
     4741 gcaacgctga aagcagggcg gcgccgaagc tggccgcatg cacgccgatc tcgcgctggc
     4801 tcacgaggca attgcgccgg ggatgcacga actcgatcgg atcttcgacc gtgaggatat
     4861 ggcctttcac gcgcttgttg atatcgtcga tcatcgctgc gagcgatgtg gatttgccgg
     4921 aaccggtctt gcccgtcacg aggatcaaac cactcactgc gtggcacagc tgccggatcg
     4981 cagtcggcat attgagctgg tcgagcgtca gcgcctgtga agggatggcc cgaaacacgg
     5041 ctcccatgcc attgagctga cgcatgacat tgacacgaaa tcgtcccttg ccctcgagcg
     5101 tgtaagcgaa atccgcgcca tccttgctct cgaagcgctc cagcgccttg cgcggcatga
     5161 tttcgtagat tgccgccttg caggcctcag gcgcggtcgg ttctgcgcgc agcggcgaaa
     5221 gatcgccgtg caggcgtatg cggggcggat cgccagcaat gaagtgcaga tccgatccgc
     5281 ggcgtttgag gatctcttcc agcagggagt cgatctgcgc cacgctttag ctgcctgttt
     5341 gtgacatcgc agccgccggc aactcgcgtg gtatcagcgt cggatccgtc acgaatttct
     5401 ggaacagtcg cttgtcgctc gcgtagacca ctgcatcatc cggatcgatc tctttcttct
     5461 cgagtgactg caacagcgcc tgatcgaaca tctgcatacc gaattccttg cccatctgca
     5521 gctggctcgg aatctggtgc gtctgatcgg tagaaatcaa tttgccgatt gccttggtgg
     5581 ttaccatgac ctccacgacg gcgcgccgtc cgcggccgtc cgcggtcttg acgagaatct
     5641 gcgtgactac ggcgagcagg ctctgcgaca ggaagctctt ggtctgctcg cgctcctcgg
     5701 cgggcagagc atcgatcatg cgatcgatgg tcttgaccgc gctggtggta tgcagtgttc
     5761 ccagcacgag gtggccggtt tctgcagcgg tcatcgccat acgaatggtc tccgcatcgc
     5821 gcaactcgcc gaccaggatc acatcgggat cctcgcgcat cgcggcgcgt acgccttcgg
     5881 cgaaactcgg gatgtgtgta ccgagttcgc gctgaatgac ctggctgctc ttgctgcggt
     5941 ggacgaactc gatcggatcc tcgaggctga tgatattgac gggcctggtg ctgttgagca
     6001 gatcgatcat cgcggccaga gtggtggact tgcctgtgcc ggtcgagccg gtgaccagga
     6061 tcattccctg gtggtagtca cagagcttgg taacgatggg cggcagtccg agctgcgcca
     6121 gattcggaac ctcggtggga atcgcccgga acgctgcgcc cacgccggtg tccttgcgat
     6181 agacgttgac gcgaaagcgt ccgccttcgg ccgacacata cgagaagtcc agatcgttgc
     6241 cccgcagaaa gtgctcctgc tggctggggg tcaggatctc ggtaacgtag ctctcgagct
     6301 cggtatcccg aaggtcacga aatttgatcg ggaccaggtc accgtgcatc cgcagcatag
     6361 gcgggacgcc tacggccaaa tggatgtcgg agcacccctg ctgggtgccg agttttagga
     6421 acgcatcaac acgcgccacg gctggttacc ctgaaccctc tggagcctat gcccccctgt
     6481 cgagtcctaa gctatacccc gtaaaggccc gtcgcatcaa cagattggga cctgaaactt
     6541 gactcagtgc acgaacttag gggatttcgc ccggaacctc aggagcttac aggaaacgct
     6601 ctagggcgcc cttcagttct tccatggttt ggaagcgctt ggccttgtcg accgccatgg
     6661 ctttgaccac gacttccgcc aggttggcgc tgagggttgg attgatctcc tggggggcac
     6721 gcgccttacc ctgaacgtgc tgatacatga ccgccatgtg gtcgccgcgg gagtagggcg
     6781 gcactccggt cagcatctcg tacatcatca cgccgagcgc atagatgtcg gcccgctcgt
     6841 ccaccttgcg accgaggatc tgctcaggtg ccatgtactt gggggagccg atgacgtagc
     6901 cggtccgtgt cagctgggtg tcgccctcac gctgcgctgc cgccacgccg aagtccacga
     6961 tcttgaccaa cgcatcatcg ttgatcagta cattggcggg cttcaggtcg cgatgcacga
     7021 ttccaacctg gtgcgcgaca gtcatacccg tggaaatgtc ga
//
LOCUS       DATMRM010000167         4470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2883752, whole genome shotgun sequence.
ACCESSION   DATMRM010000167 DATMRM010000000
VERSION     DATMRM010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2883752"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1639
                     /locus_tag="VNZ06_04930"
     CDS             104..1639
                     /locus_tag="VNZ06_04930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWW20127.1"
                     /translation="MNFVKLQIAAAAALLAGCTVGPNFMRPEAKLPTNWCASACQANE
                     AGQSRVTSQPVTSAWWTSFNDPELLNLENAVAGANLDLKVASLRLAESRATRTVTAAD
                     RYPSVEANAGVTRERSSANGILSSAGTLAQTPNPATAANGNNGLTVTPNQGTNASPFN
                     VFQVGFDASWELDLWGRVSREVEAADASVEIQADARRDLLLSTMAEVARDYIQLRGTQ
                     SDYTITLENLNAAKDIERVTREQNAHGLATELDVAQAASEVSREAALVPQLKQQEEET
                     INRLSFLLGEAPGALRAELESSKPVPPTPPVVPIGIPSELAQRRPDIREAEATLHQAT
                     AEVGVATADFYPRITLSGSASLQALQFSQLGDWASHQFAIGPSITLPIFEGGRLKGTL
                     NLRKLQQQEAAVTYQRVVLSAWHEIDNALTQYSSQQLRRDQLEAAVKDDQRALRLAQA
                     QYVAGTGPFLQVLDAQRNLLSGQQDLSDNITGISTTLASLYKALGGGWESTYPEAASA
                     NVQ"
     gene            complement(1651..3204)
                     /locus_tag="VNZ06_04935"
     CDS             complement(1651..3204)
                     /locus_tag="VNZ06_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWW20128.1"
                     /translation="MSMLRARIAAGALLTALAGCTVGPDYHEPKAVLPAQWTDHATQA
                     FTVDRSLTVPDDVDARWWTAFSDAELSSLVERVAGANLDVKVATQRFMQARAARGMTG
                     ADALPALQGSASYQHARSSQNGLVDISGLNGKSDYNVWQPGIDASWELDVWGRVRREI
                     ESADASVQASADLRRGVLLSAMAETACDYIQLRGVQAQQAIVRQNLEIARQSLALTQI
                     RFADGVATHLEAAEAAAQVSEIEARLPVLENQRVHLVNALSFLLGEPPRTLERELDTV
                     HPIPPVPPRVPVGLPSQLAERRPDISEAEARLHAATADVGAAVGDFYPRITLSANLSL
                     QAMHFDDLDKWSSHMFGVGPALTVPIFEGGRLKGQLELRKAQQREAMIEYQRVVLNAW
                     HEVDDAMSDYDTRQARQVKLDTAVVQDQVALENARLQYVAGATDFLNVLTVQRDLLDT
                     QQARAVSTTEVSLALVGLFKALGGGWETSFPVASSVKRDSPLRTGNRTSIDDSVSRPS
                     MTASDLDER"
     gene            complement(3201..4274)
                     /locus_tag="VNZ06_04940"
     CDS             complement(3201..4274)
                     /locus_tag="VNZ06_04940"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HWW20129.1"
                     /translation="MSESIQKRKLYIAAAVLIVIVIVVSGVVASFVFSSSQTTNDAYV
                     TADFTVVSPRVAGQVTVLSVDDNQVVHQGQLLAQIDDRDYKAAVASAEANVAMAKAAI
                     DNASASIVQQQAVIEQANAALTAARANYTFARADYNRYTDLASHGAGSVQNAQQAQSR
                     IDTAQADVSRNEATVKAARQQIAVLQSDRERAQASLGQALATLDTAHLNLSWTRIVAP
                     VDGMVGQRSVRLGAYVTAGTPLLAVVPLQQAFVIANFQETQLTSVRRGEATDIHVDSL
                     PGVVLHGTVDTLAPATGVTFAAIAPDNATGNFTKVVQRIPVKIVLRPGQPALRRLRVG
                     MSVEATIHMGGSHVDEGEEGVAR"
     gene            complement(4289..>4470)
                     /locus_tag="VNZ06_04945"
     CDS             complement(4289..>4470)
                     /locus_tag="VNZ06_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20130.1"
                     /translation="PHHDFLLLFDERVREQAMVLASADVFRLMALVAVALIVLIPLIA
                     PRVYPPSAVASSPTR"
BASE COUNT          880 a         1412 c         1345 g          833 t
ORIGIN      
        1 cgcaatgatt ccgctggcaa cgctgctttc gccagtcaaa gatagcggcc gccagtccac
       61 gccgatgcat tgatggcatt tcccactgtc gagagtcgac gtcatgaatt tcgtaaagct
      121 tcaaattgct gcggccgccg cgctgctggc tggatgtacc gtaggaccca actttatgcg
      181 gccggaagcc aagctgccga cgaactggtg cgcgtcggca tgtcaagcga acgaggccgg
      241 ccagagtcgc gtgacctcac agcctgtcac ttcggcctgg tggacctcgt tcaacgatcc
      301 agaattgcta aacctggaga atgctgttgc tggtgccaac cttgatctca aggttgcatc
      361 actcaggctt gccgagagcc gcgcgacgcg caccgtcacg gcggcggacc ggtatccttc
      421 cgtcgaggcc aatgccggcg tcacccgcga acgctcgagt gccaatggca ttttgagttc
      481 cgcgggtacg ctggctcaaa cgcccaatcc ggctacggct gcaaacggga ataacgggct
      541 gacggtaacc cccaatcagg gtactaatgc gtctcccttt aacgtcttcc aggtgggttt
      601 tgacgcgtcg tgggaactgg acctctgggg ccgggtgagt cgagaagtcg aagctgccga
      661 cgcatccgtc gagattcagg ccgatgcgcg tcgcgacctg ttgctgtcga ccatggccga
      721 agtcgcacgc gactatatcc agctacgcgg cactcaatcc gactacacca tcacgctcga
      781 gaacctgaac gccgccaaag acattgaaag ggtcacacgc gagcagaacg ctcacggact
      841 cgcaaccgag ctggatgtcg ctcaagcagc gtccgaagta tcgagagagg ccgctcttgt
      901 ccctcaactg aaacagcagg aggaagagac catcaatcgc cttagcttcc tgcttggcga
      961 agcccctggt gcgctgcgag ctgagcttga atccagcaag ccggtgccgc caacgccacc
     1021 ggtggtccct atcggcatcc cgtcagaact ggcgcagcgc cggccggaca ttcgagaggc
     1081 tgaggcaacg ctgcatcagg caaccgcgga agttggcgtc gcaacggcag acttctatcc
     1141 gagaatcacc ctctcgggca gtgccagtct gcaggcactc cagttcagtc aactgggaga
     1201 ttgggcgtct catcagtttg caatcggtcc atcgattact ttgccgatct ttgagggtgg
     1261 gcgattgaag ggtacgctca accttcgcaa attgcagcag caggaagcag cagttactta
     1321 ccagagggtc gtgctatctg cgtggcacga gatcgacaac gccctgacgc aatattcatc
     1381 gcaacagctc cgccgcgacc aactggaagc agccgtcaag gacgatcaaa gggcactccg
     1441 cctggctcag gcgcaatacg ttgccggtac aggcccattc cttcaggtgc tcgatgcaca
     1501 gcggaacctc ctttcggggc aacaggatct gagcgataac atcaccggca tctccacgac
     1561 gctggcctca ctttacaagg cacttggcgg cgggtgggaa tcgacttatc ccgaagcggc
     1621 ttctgcgaac gtgcaatgaa tgaagactca tcatcgttca tccagatctg atgctgtcat
     1681 ggagggccgc gaaacactgt cgtcgatcga ggtgcggttc ccagtacgca gcgggctgtc
     1741 gcgcttcacc gaagacgcca ccgggaagga ggtctcccac ccgccaccga gtgccttgaa
     1801 aaggccgacg agcgcgagcg aaacttctgt ggtgctgacg gctctcgcct gctgggtatc
     1861 caacagatcc cgctggaccg tgaggacgtt caggaagtcc gtagcccccg caacgtattg
     1921 aaggcgagcg ttctcaagcg ccacctgatc ctgtactacc gcggtgtcga gcttcacctg
     1981 cctggcctga cgtgtgtcgt aatcgctcat cgcgtcgtcg acctcgtgcc aggcgttgag
     2041 cacgacacgc tggtattcga tcattgcctc tcgctgttgc gccttgcgca gctcgagttg
     2101 ccctttcagc cggcctccct cgaatatcgg cacggtcagc gccggaccta cgccgaacat
     2161 gtgcgaactc catttgtcga gatcgtcgaa atgcatggcc tgaagactca ggttcgccga
     2221 gagcgtgatc cgcggataga agtctccgac cgccgcgccg acatcggctg tcgccgcgtg
     2281 caaacgcgct tccgcctccg agatatccgg acgacgctcg gccagctgtg aagggagtcc
     2341 gacgggcacg cgaggcggca ctggaggaat gggatgtacg gtatcaagtt cgcgctccag
     2401 cgttcgcggc ggttcgccaa gcaggaagct cagcgcgttg accagatgga cgcgctggtt
     2461 ttccagcacc ggcaggcgtg cctcaatttc actcacctgc gctgcagcct ctgcggcttc
     2521 gaggtgcgtt gccaccccgt cagcgaagcg aatctgcgtg agcgccaagc tctggcgtgc
     2581 gatctcgagg ttttgccgga cgatggcctg ttgtgcctgc accccccgca gctggatgta
     2641 atcgcatgcc gtctcggcca tcgccgacag caacacgcca cgacgcaggt cagcactcgc
     2701 ctgcaccgac gcgtcagccg attcgatctc gcggcgcacg cgtccccaaa catcaagctc
     2761 ccatgaagcg tcgattcccg gctgccagac gttatagtca ctcttgccgt tcaacccgga
     2821 gatgtccaca agcccattct ggctcgaacg cgcgtgctga tacgacgcgg agccctgcag
     2881 cgcgggcagt gcgtcggcac cggtcatgcc acgggccgct cgcgcctgca tgaaccgttg
     2941 cgtggcgacc ttgacatcga ggttcgcgcc cgctacgcgc tcgacgagtg aggaaagctc
     3001 cgcgtccgag aacgctgtcc accagcgcgc gtcgacatcg tccggtaccg tgagactgcg
     3061 atcgaccgtg aaagcctggg tcgcgtggtc ggtccactgt gctggcagca cggcctttgg
     3121 ctcgtgatag tccggaccga cggtacaccc ggcaagtgcc gtcaaaagtg cccctgcggc
     3181 gattcgtgcc ctgagcatgc tcatcgtgcg acgccctcct cgccttcgtc cacatgcgag
     3241 cctcccatgt gaatggtcgc ctcgaccgac atgcccacgc gaagtctccg caacgcaggc
     3301 tgcccgggac gcagtacgat cttcaccgga atgcgctgga ccaccttggt gaagttgcct
     3361 gtcgcgttgt cgggtgcgat ggcggcaaaa gtgacgccgg tggccggcgc cagggtgtcc
     3421 accgttccgt gcagcacaac gcctggaagg gaatccacgt gaatatcggt cgcctctccg
     3481 cgacgcaccg aagttagctg tgtttcctgg aaattggcga taacgaaggc ttgctgcagc
     3541 gggaccaccg caagcagtgg cgttcccgct gtgacatagg ccccgaggcg caccgagcgt
     3601 tgcccgacca tgccgtcgac cggcgccacg atacgcgtcc acgacaggtt cagatgggcg
     3661 gtgtccagtg tcgccagtgc ttgtccaagg gacgcttgcg cccgctcccg gtcggactgc
     3721 agaaccgcga tctgctgccg tgcggccttt acggtggctt cgttgcggga aacatccgcc
     3781 tgcgcggtat cgatgcgcga ttgtgcctgc tgcgcgttct ggacgcttcc cgcgccgtga
     3841 ctcgccaggt ccgtgtagcg gttgtagtca gcccgggcaa aggtatagtt cgcgcgagcc
     3901 gcggtcagcg cggcattcgc ctgttcgatg acggcctgct gctggacgat actcgccgac
     3961 gcattgtcga tagcggcttt cgccattgcg acattcgcct ccgcggaagc gaccgccgcc
     4021 ttgtagtccc ggtcgtcgat ctgggcgagc agctggccct ggtggacaac ctgattgtca
     4081 tcgaccgaga ggaccgtgac ctggccggcc acgcgcggcg aaaccacggt gaaatcggcg
     4141 gtcacatagg catcgttggt cgtctgcgac gatgaaaaga caaaactggc gaccacgccc
     4201 gacaccacga tcacgatcac gatcaggaca gcggcggcga tataaagctt tcttttttgg
     4261 atggattctg acatggctaa acgagacgtt atcgggtcgg actgctggcg acggcagagg
     4321 gcggataaac acgcggagcg atcaagggaa tcagcacgat cagcgcaacg gcaacgagcg
     4381 ccatcaaccg gaacacatcg gccgaagcga gaaccatcgc ctgctcccgc acccgctcgt
     4441 cgaacagcag cagaaagtcg tgatgaggta
//
LOCUS       DATMRM010000168         3091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1341671, whole genome shotgun sequence.
ACCESSION   DATMRM010000168 DATMRM010000000
VERSION     DATMRM010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341671"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VNZ06_04950"
     CDS             <1..254
                     /locus_tag="VNZ06_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020796668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWW20131.1"
                     /translation="TGLFIAIGHTPNTSLFENQLDMRGGYLLVKGGSEGDATATSREG
                     VFAAGDVADHVYRQAITSAGSGCMAALDADRYLERFDGA"
     gene            254..1405
                     /locus_tag="VNZ06_04955"
     CDS             254..1405
                     /locus_tag="VNZ06_04955"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HWW20132.1"
                     /translation="MQTRVLSRIDEIAASEWNAIAGDYPFMRHEFLAALENTGCANAR
                     TGWRPRHLVIQDEAGLAAAAPLYEKTHSWGEFVFDFSWARAAEQAGMRYYPKMVCAIP
                     FTPTTGPRLLCRAQANSEELRERLLLEMRERAACFHASSVHALFLDAPARSACEQQQW
                     LLRRDCHFQWRNRGYGSFDDFLNTFSADKRKKARRERRRVVEQGIEFATLRGDELSDE
                     LIERIYAFHEITFIRHGHTPYLNVEFFARVARSLPDQFVVKLGMQGKRPVAAAIFFRS
                     ADTLFGRYWGSGADFHSLHFEACYYQGIDYCIEQRLARFEPGTQGEHKLARGFEPAIT
                     WSAHWIADPSLRRAIGAYLQREGDAIDAYATQADEHVPYRKGELTQPPQ"
     gene            1402..2055
                     /gene="aat"
                     /locus_tag="VNZ06_04960"
     CDS             1402..2055
                     /gene="aat"
                     /locus_tag="VNZ06_04960"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HWW20133.1"
                     /translation="MITWLSPDDPPDRFPPHDAALRDPPGLLAAGGDLRVERLLAAYQ
                     RGIFPWYSPGQPVLWWSPDPREVILPREFHTSRSLLRARRVRGFQWYEDRNFAAVVAA
                     CAAPRARSPGTWITAEMKAAYCALHSQGFAHSYEIWRNNRLVGGVYGVRLGCVYFGES
                     MFSAERDASKAALAGLVEHCTALGIELIDCQLPSPHLRSLGSRPMPRAQFLRYLEPR"
     gene            2136..2354
                     /gene="infA"
                     /locus_tag="VNZ06_04965"
     CDS             2136..2354
                     /gene="infA"
                     /locus_tag="VNZ06_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWW20134.1"
                     /translation="MPKEDAIQMEGEVVETLPNTTFRVKLKNGHLVTAHISGKMRKNY
                     IRILTGDAVTVEMTPYDLSKGRIVYRGR"
     gene            complement(2362..>3091)
                     /locus_tag="VNZ06_04970"
     CDS             complement(2362..>3091)
                     /locus_tag="VNZ06_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWW20135.1"
                     /translation="FVRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAIAKHPHC
                     VLLLDEIEKAHPDVFNLLLQVMDHGTLTDNNGRKADFRHVIIVMTTNAGAQEMNRAAI
                     GFTQADNTSDGMEAIRRLFSPEFRNRLDAVIQFAALDGPTIERVVDKLLIEVEAQLEP
                     KGVVLTIDEAARRWIATKGYDVKMGARPMARVIQEHIKRPLAEELLFGKLSEGGGQVR
                     ISLKPDETGLNIECAPVSQPEVTA"
BASE COUNT          561 a          994 c          938 g          598 t
ORIGIN      
        1 cgaccggcct gttcattgcc atcgggcaca caccgaatac gtcgctgttc gaaaatcagc
       61 tcgacatgcg cggcggatac ttgctggtca agggtggcag tgagggcgat gcgaccgcca
      121 ccagccgcga gggcgtgttc gctgccggcg atgtcgccga tcatgtctat cggcaggcaa
      181 tcacctccgc cggctcgggc tgcatggcgg cactcgatgc cgaccgctac cttgaacgct
      241 ttgacggagc ctagtgcaga ctcgcgtact ctcgcgcatc gatgaaatcg ccgcttccga
      301 atggaacgcg atcgccggcg actatccgtt catgcgacat gagttcctgg cggccctgga
      361 aaacacgggc tgcgccaacg cccgcaccgg ctggcgtccg cgtcacctgg tgatccagga
      421 cgaggcagga ctcgcggccg ccgcgccgct gtatgagaag acgcattcct ggggcgaatt
      481 cgtattcgat ttctcgtggg ctcgcgccgc cgaacaggcg ggcatgcgtt attacccgaa
      541 aatggtgtgc gcgattccat tcacgccaac caccggtcct cgcctgctgt gccgcgcgca
      601 ggccaacagc gaggagctgc gcgaacgcct gttgctggag atgcgcgagc gcgcggcctg
      661 ctttcatgcc tcgtcagtcc acgcactgtt tctggatgcg ccggcacgca gtgcctgcga
      721 gcagcagcag tggctgctgc ggcgcgactg ccactttcag tggcgcaacc gcggctacgg
      781 cagcttcgac gactttctga atactttctc cgccgataag cgcaagaaag ccaggcgcga
      841 acgtcggcgc gtcgtcgagc agggaatcga gttcgcgacg ctgcgtggcg acgagctgag
      901 cgatgagctg atcgagcgca tctacgcttt ccacgagatc acgttcatcc gccatgggca
      961 cacgccctac ctgaacgtcg agtttttcgc gcgcgtcgca cgctcgctgc ccgatcaatt
     1021 cgtcgtcaag cttgggatgc agggcaaacg ccccgtagcg gccgcgattt tctttcgctc
     1081 ggccgatacg ctgttcggtc gctactgggg ctcaggagcc gattttcaca gcctgcattt
     1141 cgaggcttgc tattaccagg gtatcgacta ttgcatcgag cagcgcctcg cgcgcttcga
     1201 gcctggcact cagggtgagc acaagctggc gcgaggtttt gagccagcca ttacctggtc
     1261 ggcacactgg atcgcggatc cgtcgttgcg ccgcgcgatc ggggcctatc tgcagcgcga
     1321 aggcgacgcg atcgatgcgt acgccactca agccgatgag cacgtgccct atcgcaaagg
     1381 tgaactgacc cagccgccgc aatgatcacc tggctgtcgc cggacgatcc gcccgatcgt
     1441 tttcccccgc acgatgcagc gttgcgcgat ccgccggggc tgctcgctgc cggcggagat
     1501 ctgcgcgtcg agcgcctgct tgccgcctat cagcgcggaa ttttcccatg gtattcaccc
     1561 ggccagccgg tgctgtggtg gtcgccggac ccgcgcgaag tgatcctgcc gcgcgagttt
     1621 catacctcgc gcagtctgtt gcgcgcgcgg cgcgtgcgcg gcttccaatg gtacgaagac
     1681 cgcaatttcg ccgcggtggt cgcagcctgt gcagctcccc gggcccgcag ccccggcacc
     1741 tggatcaccg ccgaaatgaa ggccgcctat tgtgctctgc acagccaagg attcgcgcac
     1801 agctatgaga tctggcgtaa caaccgcctg gtgggcggcg tctacggtgt gcgtctgggg
     1861 tgcgtttatt ttggcgaatc aatgttcagc gctgaacggg acgcctcgaa ggcggcgctc
     1921 gcaggtctgg tggaacattg caccgccctc ggtatcgaac tgatcgactg ccagctacca
     1981 tccccacacc tacgcagtct gggtagtcgt ccgatgccac gggcgcagtt tctgcgctat
     2041 ttggagcccc gctagagccg gaaatcttcg aggcacattg cattggcacc gccgtttgtg
     2101 cgtaaattcg cggcccttgg gccacgcaac gcagcatgcc gaaagaagac gcaatacaaa
     2161 tggagggcga ggtcgtcgag accttgccga atacgacctt ccgcgtgaag ctgaagaacg
     2221 gccacctggt gaccgcccac atctccggca aaatgcgcaa gaactacatc cgcatcctga
     2281 cgggcgatgc agtcaccgtc gaaatgacgc cgtacgatct gtcgaagggc cgcatcgtct
     2341 accgcggccg ctagtttaac ctcaggcagt cacctcaggc tgtgagaccg gggcgcattc
     2401 gatgttgagg cccgtttcat ccggcttgag cgaaatgcgc acctggccgc ccccctcgga
     2461 gagcttgccg aacagcagtt cctcggccag cggacgtttg atgtgctcct ggatcacgcg
     2521 cgccatcggc cgcgcaccca tcttcacgtc atagcccttg gtagcgatcc agcgccgcgc
     2581 ggcctcatcg atcgtgagca ccactccttt gggctcgagc tgcgcttcga cttcgatcag
     2641 cagcttgtcc accacacgct cgatggtcgg gccatccagc gccgcaaact gaatcaccgc
     2701 atccagacga ttgcggaatt ccggtgagaa cagacgacgg atcgcttcca tgccatcgct
     2761 ggtgttgtca gcctgcgtaa agccaatcgc ggcgcggttc atctcctgcg caccggcatt
     2821 ggtggtcatg acgatgatga cgtgtcggaa gtcggccttg cgcccgttgt tatcggtcag
     2881 cgtgccgtgg tccatgacct gcagcagcag attgaacaca tccggatgcg ctttctcgat
     2941 ctcatccagc aacagtacgc aatgcgggtg tttggcaatg gcttcggtga gcaacccgcc
     3001 ctgatcgaaa ccgacatagc ccgggggggc accgatcagg cgtgaaacgg tgtgccgctc
     3061 catgtactcg gacatgtcga aacgtacgaa c
//
LOCUS       DATMRM010000169         3643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2577731, whole genome shotgun sequence.
ACCESSION   DATMRM010000169 DATMRM010000000
VERSION     DATMRM010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3643
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2577731"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..888
                     /locus_tag="VNZ06_04975"
     CDS             193..888
                     /locus_tag="VNZ06_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HWW20136.1"
                     /translation="MSRAERLLQLMQVLRRHRFPVSGALLAGELRVSLRTLYRDIASL
                     QSQGARIDGEAGLGYVLRPGFLLPPLMFSQDEIEALVLGSRWVADRADSRLADAARGA
                     ISKVAAVLPADLRIELDTSALLIAPSRQIAAGDEELATIRQAIRSERKLVVKYRDLKE
                     QESVRTIWPFALAFFDRVRVVVAWCELRRDMRHFRTDRILALSVSKTKYPRRRHTLLK
                     EWRQIEGFETLFP"
     gene            complement(933..1925)
                     /locus_tag="VNZ06_04980"
     CDS             complement(933..1925)
                     /locus_tag="VNZ06_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWW20137.1"
                     /translation="MSRALLAFALALCGLDVGVAAERPEWAFFVPADAPPPKGSALSD
                     PFPAQWSAPGSTRSYSGAQLQDPLNPPDWYPSEHPQMPPVVAHGSKAEKEGPPVLPCA
                     LCHLPNGAGHVESASLAGLPSNYILHQLDDIRSGTRQITVGGPHAISLITTLKKGFAQ
                     EQLPAAARYFSSLKPRTWIRVVEAYAVPKSFVSPDSLMRLPVPNGGTEPLGRRIVELP
                     ESALGLIARDSHSGYVAYVPEGSIAAGEVLVNKGGPGRTLACASCHGAKLTGLGDVPA
                     LAGRPPSYIARQLWAFQSGARAGSLSAGMKPVVANLTAEEMLDIAAYLASVPPR"
     gene            1989..2960
                     /locus_tag="VNZ06_04985"
     CDS             1989..2960
                     /locus_tag="VNZ06_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWW20138.1"
                     /translation="MSTWRYRKGLHDLGNGGYAYLQPDGTWGWSNAGLIESRGETLLV
                     DTLMGLAITRDMLSEMRGKVPAAASIRRLVNTHANADHVLGNELVTGAEIIGTRATAE
                     DVARMNPDAIAAMAKNWRQMGEGAEFFFETMGRTFDFSGVTVTPPTTLFERELELRVG
                     DKIVRLIDLGPAHTRSDLIVYVPADKLVFTGDLLFNQGHPIMWEGPVQNWIGACHYIE
                     ALDLDTVVPGHGPICDKAAVREMRHYFEYLRDQARARYDAGLSYEEAARDIAMDAFGT
                     WTDPERVVGNVFVLYREFGAKLEAPGPLQVFDLLARHSKWLRERRAS"
     gene            complement(2971..>3643)
                     /locus_tag="VNZ06_04990"
     CDS             complement(2971..>3643)
                     /locus_tag="VNZ06_04990"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HWW20139.1"
                     /translation="REAQVVQWLSSQASYQRAFAQTFAADPTPVSVENVIKAIAAYER
                     TLISGHSAFDRYVFEDDQSALSPSAKRGMALFYSDRVGCGQCHSGINFDGPVRFKGHE
                     QVSALFANTGLYSVDGCGGYPQSDQGLFEVTRKKTDVGKFRVPTLRNVALTAPYMHDG
                     SIATLGEVIDHYAQGGRQLPGGPSALNHLVDRRIRAFKISPEEKDDLIAFLEGLTDPR
                     FVRTQ"
BASE COUNT          707 a         1103 c         1120 g          713 t
ORIGIN      
        1 acccggcaat gacggcgcgc aaatcaagct gccgacgcca aggcgccaca gtatccggtt
       61 gagcatgcgc aattagacta ccgtaccaat gttgaaaact gcttgcgctt gaccatcgct
      121 ctgagtgccc gatgcgcatg ccgccagttt ttggcagtat atccgaagcg ccccggatcg
      181 gttgagtcag ctatgtcgcg cgccgagcgc ctccttcagc tcatgcaggt cttgcgtcgc
      241 caccgtttcc ccgttagcgg cgcgttgttg gccggtgaac tgcgcgtgag cctgcgtacc
      301 ttgtatcgcg atatcgcatc gctgcaatcg caaggcgccc gcatcgacgg cgaggcgggt
      361 ctgggatatg tgctcaggcc gggctttctg ctgccgcctc tgatgttttc ccaggatgag
      421 atcgaggcgc tggtgctcgg ctcgcgctgg gtcgcggatc gcgctgactc ccggctggcc
      481 gatgcggccc gcggcgccat ctcaaaggtc gcagcggtgt tgccggcgga tttgcgcatt
      541 gagctggata cctcagcact gctgatcgcg cctagccgac agatcgctgc aggcgatgag
      601 gaactggcca cgatacgcca ggctatccgc tcagagcgaa agctggtcgt caaatatcgc
      661 gacctgaagg agcaggaatc ggtacgcacg atctggccct tcgcactcgc gttcttcgat
      721 cgcgtccgag tcgtggtcgc gtggtgtgaa ttgcgccgtg atatgcgcca ctttcgtacg
      781 gatcggattc tggctttatc ggtgtcgaag acgaaatacc cacgtcgccg tcatacgctg
      841 ctgaaggagt ggcggcagat cgagggattt gagactctct ttccttgagg cagctcaatt
      901 gctgccagtg tctggcagca cgcccgctga agctatctgg gcggcaccga tgccagatag
      961 gcggcgatgt ccagcatctc ctcagccgta agattcgcga cgaccggttt cattcccgct
     1021 gacagcgagc ccgctctggc gccgctttgg aaggcccaga gctgtcgtgc gatgtaactg
     1081 ggcgggcgac cggccagcgc tgggacgtcg cccagtccag tcagtttggc gccatggcaa
     1141 ctggcacagg cgagagttcg acccggcccc cctttgttca ccagcacctc gccggctgcg
     1201 atgctgcctt caggcacata cgcgacatag cccgagtgcg aatcgcgggc gatcagcccc
     1261 agcgcgctct ctggtagttc gacaatgcgt cgccccagcg gctcagtgcc cccgttgggg
     1321 acaggcagcc gcatgagcga gtccggactc acgaacgact ttggcaccgc gtacgcttcc
     1381 actacgcgaa tccaggtacg cggtttgagc gaggagaaat atcgcgccgc tgccggcagc
     1441 tgctcctgcg caaaaccctt cttcagcgtg gtaatgagac tgatcgcatg cgggcccccg
     1501 acagtgatct gtcgcgtgcc gctgcgaata tcatcgagct gatgcaggat gtagttcgac
     1561 gggagacccg ccagcgatgc ggactcgaca tgtccggcac cgttcggcag atgacacagt
     1621 gcgcaaggca ggacaggcgg tccctccttc tcggcctttg atccgtgtgc gacgaccgga
     1681 ggcatctgcg gatgctctga agggtaccag tctggcggat tcagaggatc ctgcagttga
     1741 gcgccgctat aggagcgtgt gcttcccgga gccgaccact gtgcgggaaa cgggtccgag
     1801 agagcgctgc ccttgggggg cggcgcgtct gcaggcacga agaacgccca ttcgggccgt
     1861 tccgcggcga cgccgacatc aagaccacac agtgcgagtg cgaaggccag cagtgctcga
     1921 gacatgagcc cgccattttg gagcacccgt gctttaatga caaagcaaat cccggccagg
     1981 tgagcactat gtcgacatgg cgatacagaa aaggactgca cgatctgggc aacggcggat
     2041 atgcctatct tcaaccggac ggaacctggg gctggtccaa cgccggattg attgagagcc
     2101 gaggtgagac actgctggta gacacgctga tgggtcttgc catcactcgc gacatgttgt
     2161 ccgaaatgcg aggaaaagtg ccggcggccg cttccatcag gcgcctcgtc aacacgcacg
     2221 ccaatgcgga tcacgtgctt ggcaacgaac tggtgacagg tgccgagatc attggaaccc
     2281 gggccaccgc ggaagatgtg gctcgaatga accccgacgc gatcgctgcc atggcgaaga
     2341 actggcgcca gatgggcgag ggtgcagagt tcttctttga aaccatggga aggacgttcg
     2401 atttttccgg ggtgacggtc acgcccccga caacgctatt cgagcgcgag ctagaactgc
     2461 gcgttggcga caagatcgtc cggctcatcg atctcggtcc ggcgcacacg cgctccgacc
     2521 tcatcgtcta tgtgcccgcg gacaagctgg tgtttaccgg tgacctgcta tttaaccaag
     2581 gtcacccgat catgtgggag ggaccggtgc aaaactggat cggcgcctgt cactacatcg
     2641 aggcgctgga tctggacacc gtcgtaccgg gacatggccc gatctgtgac aaagccgcgg
     2701 tgcgtgaaat gcgccattat tttgagtatc tgcgcgatca ggcgcgcgcg cgatacgacg
     2761 ccgggctcag ctacgaggaa gcggcccgcg acatcgccat ggatgccttc ggaacctgga
     2821 ccgatcccga acgggtcgtc ggcaatgtgt tcgtgctgta tcgtgaattt ggcgcaaagc
     2881 ttgaagcacc ggggccgctg caggtgttcg atctgcttgc gcgtcacagt aagtggctac
     2941 gcgaaagacg tgcctcttag cagaccgcgc tcattgcgtg cgtacgaacc gcggatcggt
     3001 cagaccttcg agaaacgcga tcagatcgtc cttctcctcg ggcgagatct tgaacgcacg
     3061 gatgcgtcga tccaccaaat gatttagtgc gctcggtcct ccaggcaatt gtcgcccgcc
     3121 ttgcgcatag tgatcgatga cctcgcccaa cgtcgcgatg ctgccatcgt gcatgtaggg
     3181 tgcggttagc gcgacgttgc gcaaggtcgg cacacgaaat tttccgacgt cggtcttctt
     3241 gcgagtgacc tcaaacagcc cctgatcgct ttgcggatag ccgccgcacc cgtccacgct
     3301 atacagtccg gtgttggcaa aaagggcgct cacttgttcg tggcctttga atcgcacggg
     3361 cccatcgaaa ttaatgccgg agtgacactg cccgcagccc acacggtcgg aatagaacaa
     3421 cgccatgccc cgcttcgcac tcggcgagag tgcactttga tcatcctcga acacatagcg
     3481 atcgaacgcc gagtgcccgg atatcagcgt tcgttcatag gccgcgatcg ctttgatgac
     3541 gttttccact gaaaccggtg tcggatcggc ggcaaacgtc tgcgcgaatg cccgctgata
     3601 gctcgcctgc gacgacagcc attgaaccac ctgcgcctca cgc
//
LOCUS       DATMRM010000170         5047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3811411, whole genome shotgun sequence.
ACCESSION   DATMRM010000170 DATMRM010000000
VERSION     DATMRM010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3811411"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VNZ06_04995"
     CDS             complement(<1..449)
                     /locus_tag="VNZ06_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWW20140.1"
                     /translation="MKVILAGAGAFGIKHLEAMAKINGIEVASIVSRDTQATAEVARH
                     WRIPHYTTDLAEALEQPGVEAALLCTPTQMHARQAVQCLRAGKHVMIEIPIADSLADA
                     ELVVKTQKQTGLTAMGGHTRRFNPSHQWIHRRVHSGELKVLQMDVQT"
     gene            complement(460..1299)
                     /locus_tag="VNZ06_05000"
     CDS             complement(460..1299)
                     /locus_tag="VNZ06_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit beta"
                     /protein_id="HWW20141.1"
                     /translation="MARIYAGLATSHVPAIGAAIDNGRTHEPYWQPLFEGYEYAKQWI
                     VGHMPDVVILVYNDHASAFSLDLIPTFALGCADEFAPADEGWGPRPVPVVKGHAPFAA
                     HIAQSLILDEFDLTIVNKMDVDHGLTVPLSLVFGTPAAWPCRVIPLAVNVVQYPPPTG
                     NRCFNFGRALGRAVESYPQELRVMVWGTGGMSHQLQGPRAGLINKVWDNRFLDRIGPD
                     PRSLARIPHLEYLREAGSEGIELVMWLIMRGALQDSARTVHRFYHVPASNTAVGHLIL
                     ENQ"
     gene            complement(1292..1699)
                     /gene="ligA"
                     /locus_tag="VNZ06_05005"
     CDS             complement(1292..1699)
                     /gene="ligA"
                     /locus_tag="VNZ06_05005"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: protein motif:HMM:NF009917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018579 - protocatechuate 4,5-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HWW20142.1"
                     /translation="MREPHDYDDIPGTTVFDAEQSRRGYHLNMFCMALMKADARAAFK
                     ANEAQFLQRFPMSDEQREAVLKRDWNRMIALGGNIYFLSKLFSTDGKSFQYTAAQMTG
                     MSQEAYAQMMLDGGRSPDGNRSLSEWADRKDSG"
     gene            complement(1705..2586)
                     /locus_tag="VNZ06_05010"
     CDS             complement(1705..2586)
                     /locus_tag="VNZ06_05010"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWW20143.1"
                     /translation="MANIPTCRAPDPNTRRPRFEPPPGACDAHCHIFGPGDRYPYAPD
                     RTYTPPDAPLSRFKQLQSILGLSRAVLVNASCHGRDNTVIVDAIAQSDGKYRGVANAD
                     DSFTEREFHTLHAQGFRAVRFNFVKHLGGVPDMEEFRSVLARIQPLGWHLDLHFDAGD
                     LLQFDALLNSLPVPFIIDHMGRVPTNQGLEQAPFKQLLKLLRTNERCWVKVSGSERIS
                     SGGPAFADAVPFAQALIAAAPERILWGTDWPHPNISRHMPNDGDLVDLIPLMLPDTAM
                     QRQVLVDNPHRLYHFEG"
     gene            complement(2606..4369)
                     /locus_tag="VNZ06_05015"
     CDS             complement(2606..4369)
                     /locus_tag="VNZ06_05015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWW20144.1"
                     /translation="MERSNHKASVRSCAIAALTLTCTVVAAETDKVSAPGRYSGYSQA
                     AYDDYQRSSFYLPVRDGTRLAVDLFRPTRAGQVASEPLPVVWMHTPYNRRHYAGAATV
                     ERYPGYALQLVKYGYNVAVVDFRGLYASFGRNRSYNRGEWLEPARTDAYDVTEWFARQ
                     PWSNGAIGMWGCSATGGSQMQAATTLPPSLKAIMPMSAEFDVYAFEVLGGVANPGPVM
                     TSGGTTPNANALRDAQAVAVDGSDAPELLAQAVGQHRDNIESPGVVPFRDSHSAPLED
                     EWWNVSSPSTYLRQLKRSRFGVYAAANWDEAGTKPGAFLTFNNLPRADTKLIVGPQTH
                     CAWSKVEQETGLSIVTEELRFFDHWLKGIDNHVMDQRVTYYTYHAPKGQEWRTAATWP
                     LRTQRLTRYYLAAGTLDTREPEGVHRDTAPMSAPAQSTAILIKPQSGGISYQSEALTH
                     DTQVTGYPVANLWIVTDASDVDVTARIDDVSPDGESSSYQMLGRLRASERALGHAPYD
                     NLGLPWHSYRAADARALRAGEPAQLEFELLPMSYIFRTGHRIRVTVTFADPAQRTDAV
                     PTVSLLSDSHRRSYVTLPLIP"
     gene            complement(4501..>5047)
                     /locus_tag="VNZ06_05020"
     CDS             complement(4501..>5047)
                     /locus_tag="VNZ06_05020"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HWW20145.1"
                     /translation="GMFEGVFNEGNALSFITLGRNIERADMTSRIVDVRAAAMMAGPA
                     LEFGPFETVLWMSVLNSLSGYQMYRIKMRERVYGAGVVRFLLADEQFPRACVCCLRRV
                     EEVLHRLPNSGPLLRRAARTRRAIHHTRFDTLSQGDLHEFIDRLQVSLARTNDELARI
                     YFIGHSAPESMAPPMLTAITR"
BASE COUNT          941 a         1609 c         1561 g          936 t
ORIGIN      
        1 taggtctgca cgtccatctg cagcactttg agttctccgg agtgtacgcg ccgatgaatc
       61 cactggtgac tgggattaaa gcgtcgcgtg tgccctccca tcgccgtcag gccggtttgc
      121 ttctgagtct tcaccaccag ctcggcatcg gccagcgagt cggcaatcgg tatctcgatc
      181 atcacgtgct tgccggcgcg caggcactgc accgcctgtc gggcgtgcat ctgcgtcggc
      241 gtacacagca gcgcggcttc gaccccgggc tgttcgagcg cctcggccag atcggtggtg
      301 tagtgcggga tccgccagtg tctggccacc tctgcggtgg cctgcgtgtc gcgactgacg
      361 atcgatgcga cctcgatgcc gttgatcttc gccatggctt ccagatgctt gatgccgaat
      421 gcaccggcac cggcgaggat gaccttcatg gacggtacct cactgattct cgagtatcag
      481 atgcccgacc gcggtattgg acgccgggac gtgatagaag cgatgcacgg tgcgcgcgct
      541 gtcctgcagc gctccgcgca tgatgagcca catgaccagt tcgatgcctt cggatcccgc
      601 ttcacgcagg tattccaggt gcgggatccg cgccaaggag cgcgggtccg gaccgatgcg
      661 atcgagaaac cggttgtccc acactttatt gatgagtccc gcgcgcggcc cctgcaactg
      721 gtgcgacatg ccgccggtac cccacaccat cactctgagc tcctgcgggt acgactccac
      781 agccctgccg agcgcacggc caaagttgaa gcagcgattg ccggtgggcg gcggatactg
      841 tacgacgttg accgccagag gtatcacgcg gcacggccac gccgcggggg taccaaatac
      901 cagcgacagc ggcacggtca gaccgtgatc gacgtccatc ttgttgacga tcgtcagatc
      961 gaattcgtcc aggatgagcg attgcgcgat gtgcgccgcg aacggggcgt gacctttcac
     1021 caccggcacc ggacgcggac cccagccttc atcggcaggc gcgaactcat ccgcgcagcc
     1081 gagcgcaaac gtaggtatga gatcgagcga gaaggccgac gcgtgatcgt tgtagaccag
     1141 gatcaccaca tcgggcatat gcccgacgat ccactgcttc gcgtactcgt agccttcgaa
     1201 cagcggctgc cagtagggct cgtgggtgcg gccgttgtcg atcgcggctc caatcgccgg
     1261 aacatgtgac gtcgccagtc cagcgtagat cctagccact gtccttccga tccgcccatt
     1321 ccgacagcga acggttgccg tccggcgagc gcccgccgtc cagcatcatc tgcgcgtagg
     1381 cctcctgcga catgccggtc atctgcgcgg cggtgtactg gaacgacttg ccgtcggtcg
     1441 aaaacagctt ggacagaaag tagatattgc cgccgagcgc gatcatgcga ttccagtcgc
     1501 gcttgagtac cgcctcgcgc tgttcatcgc tcatcgggaa gcgctgcagg aactgcgctt
     1561 cgttggcttt gaaggccgcg cgtgcatcgg ccttcatgag cgccatacag aacatgttca
     1621 ggtgataccc gcggcgcgac tgctcggcat cgaacacggt ggtgcccgga atgtcatcgt
     1681 agtcgtgcgg ctcgcgcatc ggcatcaacc ctcgaagtga tacaagcgat gtggattgtc
     1741 caccagcacc tgccgctgca tcgccgtgtc cggcagcatc agcggaatca ggtccaccag
     1801 atcgccatca tttggcatgt gcctggatat attgggatgc ggccagtccg tgccccagag
     1861 gatgcgctcg ggcgccgccg caatcagggc ctgggcgaac ggtacggcat cggcgaatgc
     1921 cggtccgccg ctcgagatgc gttccgagcc actgaccttg acccagcatc gctcgttggt
     1981 acgcagcagt ttcagcaact gcttgaacgg cgcttgttcc agaccctgat tggtcggtac
     2041 gcgtcccatg tgatcgatga tgaaaggcac gggcagactg ttcagcaacg catcgaactg
     2101 cagcaagtcc cccgcatcga aatgcaggtc cagatgccag ccgagcggct gaatgcgcgc
     2161 cagcacgctg cggaattcct ccatgtcggg cacgccacct aagtgcttca cgaagttgaa
     2221 acgcactgcg cgaaaaccct gcgcatgcag cgtgtgaaat tcgcgctcgg tgaaactgtc
     2281 gtcggcgttc gccacacctc gatatttgcc atcgctttgc gcgatcgcat cgacgatgac
     2341 cgtattgtcc cgtccgtgac aactcgcgtt caccaatacc gctcgcgaca agccaaggat
     2401 ggactggagt tgcttgaagc gcgacagcgg cgcgtccggc ggggtgtaag tccggtccgg
     2461 cgcatacgga tagcgatcgc ctggcccgaa gatgtggcaa tgcgcatcac aagcgcccgg
     2521 cggaggctcg aatcgcggcc tgcgagtatt cggatcgggc gcccgacagg tgggtatgtt
     2581 agccatgaag tgactatacc tgtcgtcagg gaatcagagg cagagtgaca taggagcgcc
     2641 gatgcgaatc actcagcagc gacaccgtgg gaaccgcatc ggtgcgctgc gccgggtcgg
     2701 caaacgtcac ggtgacacgg atgcgatgtc cggtcctgaa gatgtacgac atcggcagca
     2761 gctcgaactc gagttgcgca ggttcgccgg cgcgcagcgc ccgagcgtct gcagcacggt
     2821 agctgtgcca tggtagaccc agattgtcgt acggcgcatg gcccaatgcc cgctcggatg
     2881 cccgcagacg accgagcatc tgatacgacg agctttcacc gtcaggactg acatcgtcaa
     2941 ttctcgcggt cacgtccaca tcgcttgcgt cggtcacgat ccagaggttc gccaccgggt
     3001 agccggtgac ctgcgtatcg tgcgtcaacg cttcactctg ataggaaata ccgccgctct
     3061 gcggcttgat gagtatggcc gttgactgcg ccggcgcact catgggcgcc gtgtcgcggt
     3121 ggacaccttc gggctcgcgg gtatcgagcg tgccggcagc gaggtagtac cgggtcaagc
     3181 gctgggtgcg aagcggccag gtggcagcgg tacgccactc ctgccccttg ggcgcgtggt
     3241 aggtatagta cgtgacgcgc tggtccatca cgtgattgtc gatgcctttg agccaatgat
     3301 cgaaaaagcg cagctcttcc gtcacgatcg acagcccggt ctcctgttcc accttcgacc
     3361 aggcgcaatg cgtctgtgga cccacgatca gtttggtgtc ggcacgcggc aggttgttga
     3421 acgtcagaaa cgcgcccggc ttggtgccgg cctcatccca gttggcggcc gcatagacgc
     3481 cgaagcgcga gcgcttcagt tgcctaaggt aggtgctcgg actggagaca ttccaccatt
     3541 cgtcctccag cggcgcggaa tgactgtcgc gaaacgggac cacacccggt gattcgatat
     3601 tgtcgcgatg ctgcccgacc gcctgtgcga gcagttccgg cgcgtcgctg ccgtccaccg
     3661 cgaccgcctg tgcgtcgcgc aacgcgttgg cgttcggcgt ggtgccgccg gaggtcatga
     3721 cgggacccgg gttggcaacg ccccccaaca cttcgaatgc gtagacgtca aattccgcgc
     3781 tcatcggcat gatcgctttc agcgaaggcg gcagcgtcgt ggccgcctgc atctggctgc
     3841 cgccggtcgc ggaacaaccc cacattccga tcgcgccatt gctccagggt tggcgcgcaa
     3901 accattccgt gacgtcgtag gcatcggtgc gcgcgggctc gagccactcg cctcggttgt
     3961 acgatcgatt gcggccgaag gaggcataga gcccgcgaaa atccaccacc gcgacgttgt
     4021 agccatactt caccagttgc agcgcatatc ctggatagcg ctcgaccgtg gccgcgccgg
     4081 cgtagtggcg acgattgtaa ggcgtgtgca tccacacgac cggcagtggc tcgctcgcca
     4141 cctgtccagc gcgcgtcgga cggaacagat ccaccgccag ccgcgttcca tcacgcaccg
     4201 ggagatagaa tgagctgcgc tgatagtcgt cataggcggc ttgcgagtag cccgaatagc
     4261 gacccggcgc cgaaaccttg tccgtttcgg cggcgacgac agtgcacgtc agcgtgagcg
     4321 ccgcgattgc gcacgaccgc actgacgcct tgtggttgct gcgctccatg cattggtccc
     4381 cagggccacc cgcgtgtgtg caatatagtt tgggtgacca actaaataca agtttcaggc
     4441 gtgagcgccc ccgattgcgc cgcacccgcg aatcgcagga ttgcgccggc taccttaggg
     4501 ttatcgggta attgccgtca gcatcggcgg cgccatgctc tcgggagcgc tatggccgat
     4561 aaagtaaatg cgcgccagct catcgttggt ccgcgccaac gagacctgca gccgatcgat
     4621 gaattcgtgc agatcgccct gggaaagcgt atcgaagcga gtgtgatgga tcgcccggcg
     4681 ggtgcgcgcc gcccgcctca gcagcggccc actgttgggg agccgatgca gcacctcctc
     4741 gacccgtcgc aggcagcaca cgcaggcacg tggaaactgc tcatcggcca acaggaagcg
     4801 caccaccccc gcaccataga cacgctcacg catcttgatg cgatacatct gatagcccga
     4861 cagcgagttc agcacgctca tccacagcac cgtctcgaac ggtccgaact ccagcgccgg
     4921 ccccgccatc atcgccgcag cgcgtacatc gacgatgcgg ctcgtcatgt cggcgcgctc
     4981 gatgttgcgt ccgagcgtga tgaacgagag cgcgttgccc tcattgaaga cgccttcgaa
     5041 catgccg
//
LOCUS       DATMRM010000171         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4128748, whole genome shotgun sequence.
ACCESSION   DATMRM010000171 DATMRM010000000
VERSION     DATMRM010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4128748"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VNZ06_05025"
     CDS             complement(<1..652)
                     /locus_tag="VNZ06_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWW20146.1"
                     /translation="MAKLTMVEAINMAHAWEMERDPSVIVLGEDVGANGGVFRATVGL
                     QARFGERRVQDTPLAEGLIAGAAIGMAAQGLKPVAEIQFMGFIYPALDQIASHMARLR
                     NRTRGRLSCPVVLRMPHGGGIHAPEHHSESLEALLCHHPGLRVVCPSSPARAYGLLLA
                     AIRDPDPVIFLEPVRLYRFSKQEVADDGAALPLGQCFLLREGSDLTLVTWGAMTIES"
     gene            complement(673..1758)
                     /gene="pdhA"
                     /locus_tag="VNZ06_05030"
     CDS             complement(673..1758)
                     /gene="pdhA"
                     /locus_tag="VNZ06_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HWW20147.1"
                     /translation="MAEPQVVARFEIPFRACLGADGAPCGELPGFVKDAELLGRMYDM
                     MVRARAFDTKAINLQRTGKLGTYPSCLGHEATQIGIGAAMHADDVLFTVYREIGIKFW
                     RGVEMLDILLYWGGDERGTRYRHTPQDFPFCVPIGSQMPHAAGAAWAMQIKGEARCAL
                     ACIGDGGTSQGAFYEAINLAGAKSLPLVCVIVNNGWAISVPVAAQTAARTLAQKAVAA
                     GVPATQVDGNDALIVREVVGEAVERARSGGGPSVIEALTYRLSDHTTSDDATRYRSNE
                     EVEAARKRDPIARLRAFLQSRGLWDEAAEQLLRRRCSEQIEAAVSQYLATPRQSTDSM
                     FDHVYAELPEALRTQREQARRYAAAGH"
     gene            complement(1789..>2251)
                     /locus_tag="VNZ06_05035"
     CDS             complement(1789..>2251)
                     /locus_tag="VNZ06_05035"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWW20148.1"
                     /translation="GRFPEVPVSDTLTLRLLMLLGRELSSLLESALRPHDLNDTDFRI
                     LICLVSQPDGGACPSDLCAFVAQSPANMTRVADALSERGLITRTTSEHDRRRTMLRIT
                     PAGEALAHSLMPLVLGITHGVFGDMPPPARRSLLDSLRALTEQIDRRDPSR"
BASE COUNT          385 a          763 c          718 g          385 t
ORIGIN      
        1 gactctcgat ggtcattgct ccccaggtga ccagcgtcag gtcactgcct tcgcgcagca
       61 gaaaacactg tcccaggggt aacgctgcgc catcgtcagc gacttcctgc ttgctgaatc
      121 gatacagccg caccggctcg agaaagatca ccggatccgg atcgcggatc gcagccagca
      181 acagcccgta ggctcgggcc ggcgatgacg ggcagaccac gcgcaggccg gggtgatgac
      241 acagcagtgc ttccaggctc tcggagtgat gctcgggggc gtggataccg ccgccgtgcg
      301 gcatgcgcag caccactgga caactcaggc gtccgcgggt gcgattgcgc agccgcgcca
      361 tgtggctcgc gatctgatcg agcgccgggt agatgaatcc catgaactga atctcggcta
      421 ccggcttgag cccctgcgcg gccatgccga tcgccgcccc tgcgatcaga ccctccgcca
      481 gcggtgtgtc ctgtacccgc cgctcgccaa agcgcgcttg caggccgacg gtcgcgcgga
      541 acacgccgcc gttggccccg acatcctcgc cgagcacgat aactgacgga tcgcgctcca
      601 tctcccaggc atgcgccatg ttgatggcct cgaccatcgt cagtttcgcc atctgcaccc
      661 gtccttaagc gatcagtggc ccgccgcggc gtagcgccga gcttgctcgc gctgggtgcg
      721 caatgcctcc ggcagctcgg catacacgtg gtcaaacatc gagtcagtcg attgcctggg
      781 agtcgccagg tactggctta ctgccgcctc gatctgttcg ctgcagcgac gtcgcagcaa
      841 ctgttcagcc gcctcatccc acagcccgcg cgactgcagg aaggcgcgca ggcgcgcgat
      901 cgggtcgcgc ttgcgggcag cttccacttc ctcgttgctg cgatagcggg tggcatcgtc
      961 ggaagtggtg tgatcgctca gtcggtaggt cagggcctcg atgacactcg gcccgccgcc
     1021 gctgcgcgct cgctcgacgg cctcgccgac cacctctcgc acgatcagcg catcgttgcc
     1081 atccacctgc gtggccggta cgccggcagc cactgccttc tgtgccaggg tgcgcgccgc
     1141 ggtctgggcg gcgaccggta ccgagatcgc ccagccgttg ttaacgatca cacagaccag
     1201 tggcagcgac ttggcgccgg ccagattgat ggcctcgtag aaggcgccct gtgaggtgcc
     1261 gccatcgccg atgcacgcga gggcgcaacg cgcctcgccc ttgatctgca tcgcccaggc
     1321 tgcgccggcg gcatgcggca tctgcgagcc gatcggcacg cagaacggaa agtcctgcgg
     1381 ggtgtggcga tagcgcgtgc cacgctcgtc cccgccccag tagagcagga tgtccagcat
     1441 ttccaccccg cgccagaatt tgatgccgat ctcgcgatag acggtaaaca gcacatcgtc
     1501 agcgtgcatg gccgcaccga tcccgatctg cgtggcctcg tggcccagac aggacggata
     1561 ggtaccgagt tttccggtgc gttgcagatt gatggctttg gtatcgaacg cgcgtgcgcg
     1621 caccatcatg tcgtacatgc gcccaagcaa ttcggcgtct ttgacgaagc ccggcagctc
     1681 gccgcacggt gcgccatcgg cgcccaggca cgcgcggaaa ggtatttcga agcgggcgac
     1741 gacctgcggt tcagccatgg gctgaatcgt agcattagcc gccgaacgtt atcttgacgg
     1801 atcgcgccgg tcgatctgct cggtcaatgc acgcagcgaa tccagcagcg agcgtcgggc
     1861 cggaggcggc atgtcgccga acacgccgtg cgtgattccg agcaccagcg gcatcagcga
     1921 atgcgccagt gcctcgcccg ccggagtgat tcgcaacatc gtgcgacgcc gatcgtgttc
     1981 gctggtcgtg cgtgtgatca gcccgcgctc gctgagcgca tccgccacgc gcgtcatgtt
     2041 ggccggactc tgtgcaacga aggcgcacaa gtcgctcggg caggcgccgc cgtcgggctg
     2101 gctgaccagg cagatcagga ttctgaagtc ggtgtcgttc agatcatgcg gccgcagcgc
     2161 gctctcgagc agtgagctca gttcgcgccc cagcaacatc agcaaccgca aggtcagggt
     2221 gtccgatacc ggcacctccg gaaagcgacc g
//
LOCUS       DATMRM010000172         2728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1951499, whole genome shotgun sequence.
ACCESSION   DATMRM010000172 DATMRM010000000
VERSION     DATMRM010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1951499"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VNZ06_05040"
     CDS             <1..973
                     /locus_tag="VNZ06_05040"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HWW20149.1"
                     /translation="DKIPLVFSRGHGQVADNFELIRLAHGISPEEFRARPYTYTVINT
                     NSPLQLDIPMADGIIDFAAAGQVLIITPFTLAGAMAPVTIAGALTLAHAEALAGLTLA
                     QIVRPGAPIVYGSFTSNVDMKSGSPAFGTPEYVKAAFGAGQMARFLGLPWRSSNATAS
                     NTPDAQAAYESQMSLWGALFGGCNFILHAAGWLESGLTTSYEKFILDVEMLQMFAEVF
                     QPVGATAADLALEAVAEVGPGGHFFGCAHTMERYRSAFYAPLVSDWRNFGQWTEDGSK
                     TATERASTIWQSTLQRYVAPARDPAIVESLNAFVDRRTAEGGAPPVS"
     gene            976..>2728
                     /locus_tag="VNZ06_05045"
     CDS             976..>2728
                     /locus_tag="VNZ06_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HWW20150.1"
                     /translation="MADLLREDVGAELTLRGMVLGILITLVFTAANVYFGLKSGLTFA
                     TSIPAAVISMALLRGFRGVTIQENNIVQTVASAAGTLGSIIFVLPGLVMIGWWTGFPY
                     GISFTICAVGGTLGVMYSIPLRRALVTNSSLPYPEGVACAEVLKVGGSESLGSGVVGR
                     HGLAAMVWGSIISSAFAVVIATQLFAGDVAQYFRVGSKGAISGFDFSLSFALLAVGHL
                     VGLWCGIAMLVGALIAWGGAVPYLTLLHPAPGSAETVGLAVWGQQVRFIGAGAIGTAA
                     IWTLVSLLRPLVGGLSEAMAASRLRRTGRGGELRRVERDIPIGIVALVTLVCMLPIGI
                     LLAHIGTVSGLGDHLILLVTGGVTYIALMSFFVAAVCGYMAGLIGASNSPLSGIGIIA
                     VLGAALLLVLFVKPLLAPSASAALVAYALFLTSVVFCAAAISNDNLQDLKTGQLVGAT
                     PWRQQVALVVGVIAGATVIPPVMDLLNKAYGFAGTPGADPAHALAAPQAALISALAQG
                     VIQRNLEWSLLGAGVLIGLGLIVLDLALKRGTRASLPPLAVGLGIYLPTSTTMMIVVG
                     AVAGWAYERRAARTADPD"
BASE COUNT          407 a          828 c          922 g          571 t
ORIGIN      
        1 agacaagatc ccgctggtgt tctcacgtgg ccacggccag gtcgctgaca acttcgagct
       61 cattcgcttg gctcacggca tctcacccga ggagtttcgc gcgcgcccgt atacctatac
      121 ggtgatcaac accaactcac cgctgcagct cgacataccg atggccgatg gcatcatcga
      181 ttttgccgct gccggccagg tgctgatcat cacgccgttt acactggccg gagcaatggc
      241 cccggtgacg atcgcagggg ccctgacact ggcgcacgcc gaagcgctcg caggccttac
      301 gctcgcgcag atcgtccggc ccggcgcgcc catcgtatac ggtagcttta cgtcgaatgt
      361 cgacatgaaa tccggctcac cggccttcgg cacgccggag tacgtcaagg ccgcctttgg
      421 cgcgggccag atggcgcgct ttctggggct tccgtggcgc tcatcgaacg ccaccgcgtc
      481 gaatactccc gatgcccagg ccgcctacga atcgcaaatg agcctgtggg gcgcgctgtt
      541 tggggggtgc aatttcatcc tgcacgcagc cgggtggctc gagagcggac tgacgacaag
      601 ctacgagaag ttcatcctcg atgtcgagat gctgcagatg ttcgcagaag tgttccagcc
      661 ggtcggcgcc acggccgcgg acctggcgct tgaggcagtc gccgaagtgg ggccgggcgg
      721 gcatttcttc ggatgcgcgc acacgatgga gcgctatcgc agcgccttct acgcgccgct
      781 ggtttccgac tggcgcaatt ttggccagtg gaccgaggac gggtccaaga ccgccaccga
      841 gcgcgcaagc acgatctggc aaagcacctt gcaacgctat gtggcgcccg cacgggatcc
      901 ggcgatcgtg gaatcactga acgcgtttgt cgatcggcgt acggccgaag gcggcgcccc
      961 gcctgtcagt tgagcgtggc ggatctgctg cgcgaggatg tcggcgcgga gctgacgctg
     1021 cgcggcatgg ttctcggcat cctgatcacg ctggtgttta cagccgcaaa tgtctacttc
     1081 gggcttaaat ccggcctgac gtttgccacc tcgattccgg ctgccgtgat ctcgatggcg
     1141 ctgctgcggg gatttcgcgg cgtgacgatc caggaaaata acattgtgca aaccgttgcg
     1201 tcggccgcgg gcacgctggg ctcgatcatc ttcgtgctgc cggggctcgt gatgattggc
     1261 tggtggacgg gatttccata cggtatatcg ttcacgatct gcgccgtggg tggcacgctc
     1321 ggtgtcatgt attcgattcc gttgcgccgg gcactggtca cgaactcgag tctgccgtat
     1381 ccggaagggg tggcctgcgc ggaggtgctg aaggtcggcg gcagcgaatc gctgggatcg
     1441 ggcgtcgtcg gacgccacgg cctcgccgcg atggtgtggg gctcaatcat ttcgtcggcc
     1501 tttgcggtcg ttatcgcgac gcaactgttt gcgggcgatg tggcgcaata ttttcgtgtc
     1561 ggcagcaagg gcgcgatcag cggcttcgat tttagcctgt cattcgcgct gctggccgtc
     1621 gggcacctcg tcgggctgtg gtgcggaatc gcgatgctgg tcggtgcgct cattgcctgg
     1681 ggcggcgcgg tgccgtatct gaccctcctg caccctgctc ctggcagcgc ggaaaccgtg
     1741 gggctcgcgg tctggggtca gcaggtgcgc ttcatcggcg caggagcgat tggcaccgcg
     1801 gcgatctgga ccctggtttc cctgttgcgg ccgctcgtcg ggggcctgtc cgaggcgatg
     1861 gcggcgtcac ggctgcgtcg cacgggccgc ggcggggaac tgcggcgggt tgagcgcgac
     1921 atacccattg gaattgtcgc tctcgtgacg ctcgtgtgca tgttgccgat cgggatactg
     1981 ctcgcgcaca tcggcacggt gagtggtctt ggggatcatt tgatactgct ggtcacgggt
     2041 ggtgtcacct atatcgcgct gatgagtttc tttgttgccg cggtctgcgg ctatatggcg
     2101 ggcctcatcg gcgcatccaa cagcccgctg tcgggaatcg ggatcatcgc ggttctgggc
     2161 gcggcgcttc tgctggtgct gttcgtcaaa ccgttgctgg cgccctctgc cagtgcggcg
     2221 ctggtcgcct acgccttgtt cctcacctcc gtcgtgtttt gtgccgccgc gatttccaac
     2281 gacaacctgc aggatctgaa aaccggccag ctggtcggag ctaccccgtg gcgacagcag
     2341 gtcgctctgg tcgtgggcgt catcgccggg gcgacggtca tcccgcctgt catggatctg
     2401 cttaataaag cctatggctt tgccgggaca ccgggcgcgg acccggccca cgcacttgcg
     2461 gcaccacagg ccgcgctgat ttcggcgctg gcgcagggcg ttatccagag aaatctcgaa
     2521 tggagcctgc tgggtgccgg cgtattgatc ggtcttggac tgattgtgct ggatctggcg
     2581 ttgaagcgcg gcactcgcgc atcgctcccg ccgctcgcgg tcggcctggg gatctatctg
     2641 ccaacgtcca cgacgatgat gatcgtcgtg ggtgcagtgg ccggctgggc gtatgagcgg
     2701 cgcgcggcgc gaactgctga tcccgatg
//
LOCUS       DATMRM010000173         6444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1344945, whole genome shotgun sequence.
ACCESSION   DATMRM010000173 DATMRM010000000
VERSION     DATMRM010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6444
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344945"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1295
                     /locus_tag="VNZ06_05050"
     CDS             174..1295
                     /locus_tag="VNZ06_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20151.1"
                     /translation="MINSVYRNGYFGERQREPQRIGGTVPNDAMKERSVRGAGRRARS
                     AAIALMAPVLALSYPAACGADSPALAPRIAAATATAHNNRTCVAARPFYWEIGDQHAA
                     LASGTEGAAVPTASTTLPISTASEWIFGAYLVQARQGKLSAADIEALTMRSGYSNLQY
                     DRCVQRAPKTPRAVTVRDCFHAAHLVGGGNSDFKPAQVDKFFYNGGHFQQLAANDPRL
                     ADLSATALTGVIGGQLGTASAFSYDSPEPDAGIRTTSGAYAGFLRRILSGQLLIRDQL
                     GAEAVCTNPGRCQTALSTPLPQTENSRYSLGHWVEDDPRVGDGAFSDPGLHGFYPWID
                     ASKTYYGLVGRVSPAPGAHVVSMQCGRSIRKAWFAARVQ"
     gene            1373..2002
                     /locus_tag="VNZ06_05055"
     CDS             1373..2002
                     /locus_tag="VNZ06_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW20152.1"
                     /translation="MENVTDRKALVAIIDDDELLIKLLSAVLTTAGFAIQSHTSPRSF
                     VDCYDPDQPGCVVSDIQMPEMTGLELQQHLNLLGAVSPVIFLTGTADVSMAVEAMRHG
                     AFDFLEKPIGNQALVDAVRRAIEHDATNRKQLAQLGRIRARFDSLTRREHDVYRLLAE
                     GFANKQIAAELNIAGRTVELYRARVMEKMNVRSLAQLLRMKIELEKHPH"
     gene            2196..2387
                     /locus_tag="VNZ06_05060"
     CDS             2196..2387
                     /locus_tag="VNZ06_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20153.1"
                     /translation="MADRSGSALEVGDCVTFERSMIPAIGTVAELLDDDYVKVRWHDM
                     SIATTHRQLALIKLEALVP"
     gene            2384..2944
                     /locus_tag="VNZ06_05065"
     CDS             2384..2944
                     /locus_tag="VNZ06_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW20154.1"
                     /translation="MSEHQPIIDLIEDYGRVATDLREALISMGLRVRTHATVARYLAR
                     DDATDAGCLLCSIRLPGTTGRRLQEYLNFIGAATPVVLVGDSVDMGVAVDAIQLGVFD
                     YLELPLDHVRLADCVQRALDYAQRQQTHLLDLICQDVQYYDDEQRLKLQGAAAAMSGD
                     PQLNRFSDFMSELAHIRPSAAARRLH"
     gene            complement(2948..4084)
                     /locus_tag="VNZ06_05070"
     CDS             complement(2948..4084)
                     /locus_tag="VNZ06_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW20155.1"
                     /translation="MRMDDSVPRLLIVDDEPRNVTALIQTLSGNRYAVSGAHSAVEAL
                     GLLRSQAFDLLLTDLMMPQTNGIDLLREALTIDPHLVGIVMTGHGTVDTAVEAMKSGA
                     HDYILKPFNLTMILPVLERTLATRQLRIENARLVARLTHRSAELEAANEALHSANAEL
                     DSFAHSISHDLRTPLNAVIGFAELLLDAGTGPLNEAQHTYLTHIVEGGGRLRDLAEDL
                     LRFARLGQQPLSKELVDLQAMIEGIVAQLRTAQPDRPVEVRIQPLPRAQADPSLIRQV
                     FFNLLGNAFKYTRQVPHPVIEMRSHDEGDTQLYCIRDNGAGFDMRDAKRLFAPFQRLH
                     RADQFEGTGVGLSLVKRIVERHGGRIAAVGEPGKGAEFTLTLPK"
     gene            complement(4081..4260)
                     /locus_tag="VNZ06_05075"
     CDS             complement(4081..4260)
                     /locus_tag="VNZ06_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20156.1"
                     /translation="MIRCLSTLLDIRDTRELLGSRADQLALNLSDRSYVQQGLAQQTA
                     PGLWQLMRAANGLSA"
     gene            4456..4638
                     /locus_tag="VNZ06_05080"
     CDS             4456..4638
                     /locus_tag="VNZ06_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20157.1"
                     /translation="MTKRSCSYRELLMQAALQASPVVERPPADQAARQLEARCLSVFL
                     GFEALRYRLHLRRVSK"
     gene            complement(4659..5282)
                     /gene="ureG"
                     /locus_tag="VNZ06_05085"
     CDS             complement(4659..5282)
                     /gene="ureG"
                     /locus_tag="VNZ06_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HWW20158.1"
                     /translation="MDRHAEVLRLGVGGPVGSGKTALVDSLCKQLRDQLDIAVVTNDI
                     YTREDAEFLVRSQALPAERIRGVETGGCPHTAIREDASMNLAAIDGLMRQFHNLDLII
                     VESGGDNLAATFSPELSDLTLYVIDVAAGDKIPRKGGPGIMRSDLLIINKIDLAPHVG
                     ASLEVMERDARKMRGERPFVFTNLKTGYGLDKVIEFILREGMLTPLD"
     gene            complement(5312..5779)
                     /locus_tag="VNZ06_05090"
     CDS             complement(5312..5779)
                     /locus_tag="VNZ06_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWW20159.1"
                     /translation="MSHAADAVEISIADRASSGLIEALGRLLPQLSSSAPPMTLQALT
                     RILDAPSNLLLIARDPQVNGRIVGTLTLVVFPIPTAIRAWIEDVVVDESARGRGAGEA
                     LVRAAVKLAAERGAKTLDLTSRSSRRAAHRLYEKCGFHVRDSNVYRFGPKPTS"
     gene            5861..>6444
                     /locus_tag="VNZ06_05095"
     CDS             5861..>6444
                     /locus_tag="VNZ06_05095"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HWW20160.1"
                     /translation="MRVMTGLGMGLLLAATVAQSQPPARPTVVELFTSEGCNSCPPAE
                     ALIGVLVQRRPDVIALAYHVDYWDYIGWRDRFEIPEATQRQRQYQHSLHLNTVYTPQL
                     VIDGQRDVVGNNDPATVPASTHTQALALDIAVRNEQLNVSLGAGGSGSSVTPAINDVV
                     LVSYLRQGVSKVTRGENAGRELHEYNIVRSVRRLG"
BASE COUNT         1236 a         2002 c         2004 g         1202 t
ORIGIN      
        1 gctcagtctc aggcacgagc gcctgttgtg acatctagga actccagcgg gtgtccttcg
       61 gaccgatacg atacgggctt cgcctgctgg ccgcatcgga tgcggcagaa atcggcgccg
      121 attcaagacc ttattgggtc agggatgccg acatagacac ggtccccgcc ttcatgatta
      181 attccgtgta tcgcaatggt tatttcggcg aacgacaacg tgaacctcag cgcatcggtg
      241 gcacagtgcc aaacgacgcg atgaaggagc gttcggtacg tggcgcaggt cggcgcgcgc
      301 gatccgcggc gatagcgctg atggcacctg tgctggcgct cagctaccca gccgcctgtg
      361 gcgccgacag tcccgcgctg gctccccgga tcgcggccgc caccgcaacg gcgcacaaca
      421 accgcacctg tgttgctgca cggccgttct attgggagat cggagatcag cacgcggccc
      481 ttgcctccgg caccgagggc gcagctgtgc cgacggccag cacgacgcta ccgatttcga
      541 ccgcgtcgga atggatcttt ggcgcctatc tggtacaggc gcgccagggc aaactgtccg
      601 cggcggatat cgaggcgctg acgatgcgca gcggctacag caacctgcag tacgaccgtt
      661 gcgtgcagcg cgcgccgaaa acgccgcgcg cggtgacggt ccgcgactgc tttcatgctg
      721 cgcatctggt cggtggcggc aacagcgact tcaaaccggc gcaggtcgac aagttcttct
      781 acaacggcgg acactttcag cagctggcag caaacgatcc gcgcctggct gacctcagcg
      841 ccaccgcatt gaccggagtg attggcggac agctcgggac cgcgagcgcc ttcagttacg
      901 attcaccgga accagacgcc ggcatccgca cgacgtcggg cgcgtacgcc gggttcctgc
      961 gcagaattct ctcgggccag ctgctcattc gcgaccaact cggcgctgag gcggtgtgta
     1021 ccaatccggg gaggtgtcag accgcgctca gtacacccct gccgcagaca gagaattctc
     1081 gctattcgct cggtcattgg gtcgaggacg atccacgggt cggcgacggc gctttcagcg
     1141 atccgggact gcatggcttc tatccgtgga tcgacgcttc gaagacgtac tatggcctgg
     1201 tcggcagggt gagcccggcg ccgggtgcgc acgtggtatc gatgcaatgc ggtcgatcga
     1261 tccgcaaggc gtggttcgcg gctcgcgtac aatagcaagc cttgaacccg aagccactgg
     1321 tttcatgcag gccagatggt gcatgctgcg gcgtacggat tcggtgccca gcatggaaaa
     1381 cgtaactgat cggaaagctc tcgtcgccat cattgacgat gatgagttgc tgatcaaact
     1441 actctccgcc gtgctcacca ccgcgggttt cgcgatccag tctcacacgt cgccgcgttc
     1501 gttcgtcgat tgctacgatc cggatcagcc tgggtgtgtg gtgagcgaca ttcagatgcc
     1561 agagatgact ggcttggagt tgcaacaaca tctcaatctg ctgggcgccg tctcaccggt
     1621 catctttctg accggcacgg ccgatgtcag catggcggtc gaagcaatgc gacacggtgc
     1681 cttcgacttt ctcgagaagc cgatcgggaa ccaggcgctg gtggacgccg tgcggcgcgc
     1741 gatcgagcac gacgcgacca atcgaaaaca gctcgcgcag cttggacgaa tccgcgcccg
     1801 attcgattca ctcacacggc gcgagcacga tgtgtatcgc ctgctggccg aaggcttcgc
     1861 gaacaaacag atcgcggctg agctcaacat cgcaggacgc acggtcgagt tataccgcgc
     1921 acgcgtgatg gaaaagatga acgtcaggtc gttagcgcag ttgttgcgca tgaaaatcga
     1981 gctcgaaaaa caccctcact gaatctgtga ctctgaggcc gcgtcttcct gcggcgatcc
     2041 cgaatgccca cagggcttat cgcggcacag ttatgtccgc gcccgccgcg cgcagcgggt
     2101 tttgtgagcc aggcccgccc ggcttgcgga ttccacgtat ttcgaatgcc ctgagcacgg
     2161 ccgaccatac agagcaggat acgaggaaac agtcgatggc agacaggtca ggcagtgcgc
     2221 tcgaagtcgg tgactgcgtc accttcgaac gaagcatgat tccggcgatc ggtaccgtcg
     2281 ccgagctgct cgacgacgat tatgtcaagg tgcgatggca cgacatgtcg atcgcgacca
     2341 cccatcggca actcgcgctg atcaaactcg aggctctcgt cccgtgagcg agcaccaacc
     2401 gatcatcgat ctgattgaag actacggacg ggtcgcgacc gacctgcgcg aagcgctgat
     2461 ctccatggga cttcgtgtca ggacacatgc gaccgtcgcg cgatacctcg cgcgcgatga
     2521 cgcgacggac gccggctgcc tcctgtgcag catccgactt ccgggcacta cgggccgcag
     2581 gttgcaggaa tatctgaatt tcatcggcgc ggcgactccg gtcgtgctgg tgggcgatag
     2641 cgtcgacatg ggagtggcgg tcgatgcaat ccagctcggg gttttcgact acctggagct
     2701 gccgctggat cacgtgcgac tggccgactg cgtacagcgt gcgctcgact acgcgcagcg
     2761 ccagcagacc catctgctgg atcttatctg ccaggatgtg cagtactacg acgacgagca
     2821 gcggctcaaa ctacagggcg ccgcggctgc gatgagtggc gatccgcaac tgaaccgctt
     2881 ttcggatttc atgagcgagc tggcgcacat caggccatcg gccgctgcgc gccgcctgca
     2941 ctgatcgcta cttcggcagc gtgagcgtga attcggcgcc cttgccgggt tcgccaacgg
     3001 cggcgatacg cccgccatgc cgttcgacga ttcgcttcac gagcgacaat cccacgccgg
     3061 ttccctcgaa ctgatcggcc cgatgcaggc gctggaatgg cgcgaatagg cgcttggcat
     3121 cccgcatatc gaagcccgcg ccgttgtcgc ggatacagta gagctgtgta tccccttcgt
     3181 cgtgcgagcg catttcaatg acgggatggg gcacctggcg cgtgtacttg aaggcgttgc
     3241 ccagcagatt gaagaatacc tggcgtatca ggctgggatc ggcctgcgca cgcggcaggg
     3301 gctgaattcg aacctccacc ggccgatccg gctgcgcggt acgcaactgc gccacgatgc
     3361 cttcgatcat ggcctgcagg tccacgagtt ccttcgacag tggctgctgg cccaggcgag
     3421 cgaagcgcag cagatcttcg gccaggtccc gcagccggcc gcccccctcc acaatatgcg
     3481 tcaggtaggt gtgctgtgct tcattcaacg ggccggtgcc ggcgtcgagc agcagttccg
     3541 caaagccgat caccgcattc agcggtgtgc gcagatcgtg cgagatcgag tgcgcgaagg
     3601 aatcgagttc cgcattggcg ctgtgcagcg cctcattggc agcctccagt tccgccgaac
     3661 gatgcgtcag tcgggcgacc agcctcgcat tctcgatgcg aagctgccgc gtcgcgagtg
     3721 tgcgctccag caccggcaat atcatggtca gattgaacgg cttgaggatg tagtcgtgcg
     3781 ctccgctctt catcgcctcg accgccgtgt cgactgtccc gtggccagtc atcacaatcc
     3841 ccaccagatg cgggtcgatc gtcagcgcct cacgcagcag gtcgatgccg ttggtctggg
     3901 gcatcatcaa atccgtgagc agcaggtcaa aggcttgcga gcgcagcagc cccaacgcct
     3961 cgaccgctga atgcgcgccg gataccgcgt agcgattccc ggagagggtc tgtatcaacg
     4021 cggtcacatt gcgcggctcg tcatcgacga tcagcaggcg gggaaccgag tcgtccatcc
     4081 tcatgcgctc aacccgttcg cggctcgcat cagttgccac aggccgggtg ccgtttgctg
     4141 cgcgaggccc tgttgaacat aactacgatc ggacagattc agtgccaatt gatcggcgcg
     4201 actgcctagc agctcccggg tatcgcgaat gtcgagcagt gtcgacaggc agcggatcat
     4261 aacccccttg cgacgcggcg agcatcactt tgctgccgag ccgtgacctg accagtaatc
     4321 aatcggtgtc gccatggcga tggggtgtgg ggcgggtttt gtgacccgtg cacggtgttt
     4381 cttcgggtat cacgtatacc cgaaacacct cgtgcgaccg agtatgggtg ccagagcgac
     4441 aggaagggca gcaccatgac caaacgcagt tgcagctatc gcgagctact gatgcaggcc
     4501 gcgctccagg cgagtcccgt ggtcgagcgg ccgcctgccg atcaggccgc caggcagctc
     4561 gaagcgcgct gtttgagcgt gttcctggga ttcgaggcac tgcgctaccg gctgcatctg
     4621 cggcgcgtga gcaaatagca agggtcgagt cacgcggatc aatccagggg cgtcagcatt
     4681 ccttcccgca ggatgaactc gatgaccttg tccagcccat agccggtctt gaggttggta
     4741 aatacaaacg ggcgctcgcc gcgcatcttg cgcgcatcac gttccatcac ctcgagcgac
     4801 gcacccacgt gcggcgcgag gtcgatcttg ttgatgatca acaggtccga ccgcatgatg
     4861 cccggaccgc ccttgcgcgg aatcttgtcg cctgccgcaa catcgatcac gtagagcgtc
     4921 agatctgaca gctctggact gaaggtcgcc gcgagattgt cgccgccgct ctcgacgatg
     4981 atcagatcga gattgtgaaa ctgacgcatc agcccgtcga tcgccgcgag gttcatcgaa
     5041 gcatcctcgc gaatcgctgt gtgcggacag ccgccggtct ccacgccgcg gatacgctcg
     5101 gccggtagcg cttggctgcg gaccagaaac tccgcgtcct cgcgggtata gatgtcgttg
     5161 gtgacgacag cgatatcgag ctgatcacgc aattgcttgc atagcgaatc caccagcgcg
     5221 gtcttgccgg aacccaccgg gccaccgacg cccagccgca ggacttcagc gtgtctatcc
     5281 atggtgtttg attagagcat agatcgatcg ttcaggaggt cggctttgga ccgaagcggt
     5341 acacgttgct gtcgcgaaca tgaaagccgc acttctcata caggcgatgc gcagccctgc
     5401 gtgacgagcg cgatgtcaga tcgagcgtct tggcgccgcg ctcggccgcc agtttgaccg
     5461 cagcgcgcac caatgcctcg ccggcaccgc gcccccgcgc agactcatcg accacgacgt
     5521 cctcgatcca ggcgcggatc gcagtcggaa tcgggaagac taccaaggtc agggtaccga
     5581 cgatcctgcc attcacctgc ggatcgcggg cgatcagcaa cagattggag ggcgcatcca
     5641 ggattcgagt cagagcctgc agagtcatcg gtggtgccga actggaaagc tgaggcagaa
     5701 gccggccgag agcctcgatg agcccggagc tcgcccgatc ggcgatactg atttcgacgg
     5761 catcggcggc atgcgacata gaatcctcgc tgtaacccga gtgcgcagtt gagcgaactg
     5821 tcttttgaag tgcactgctg atcggtgcgg gagtcaagcc atgcgagtga tgacaggtct
     5881 gggaatggga ttattgctgg cagcgacagt cgcacagtcg cagccccccg cgcggcccac
     5941 ggtcgtggag ctgtttacgt ccgagggttg caattcctgt cctccggccg aagcgctgat
     6001 cggagtcctg gtacagcgcc ggcccgacgt cattgctctg gcttatcacg tcgactattg
     6061 ggactacatc ggctggcgtg accgcttcga gatacctgaa gcgacacagc gacagcgcca
     6121 gtaccagcac tcgctgcacc tgaatacggt ctatacgccg cagctcgtga tcgatggtca
     6181 gcgcgacgtg gtcggcaaca atgatcctgc gaccgtgcca gccagcactc atacgcaagc
     6241 gttggcgctc gatattgcgg tgcgcaatga acagctcaac gtctcgctcg gcgcaggtgg
     6301 ctcaggttcg tccgtcactc ccgcaatcaa cgatgtggtg ctcgtgagct acctgcgcca
     6361 gggggtctcc aaagtcactc gcggcgagaa cgccgggcgc gaactgcatg agtacaacat
     6421 cgtgcgttcg gtccggcggc ttgg
//
LOCUS       DATMRM010000174         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1035097, whole genome shotgun sequence.
ACCESSION   DATMRM010000174 DATMRM010000000
VERSION     DATMRM010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035097"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..2197)
                     /locus_tag="VNZ06_05100"
     CDS             complement(107..2197)
                     /locus_tag="VNZ06_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWW20161.1"
                     /translation="MASQPGSDGPGGTKRTLSDAPNGGGKRTLTDLFIGPPRDVRDPG
                     IFHSLSLVAFLAWVGLGSDGLSSSCYGPEEAFLALGHDQYLAVFLAVMTALTVFIISA
                     SYSQTIDLFPSGGGGYLVATKLLGPYPGLVSGCALVVDYVLTISISIASGGDAIFSFL
                     PTHLHWLRFWVCLLVVVVMVGMNLRGIKESVLSLLPIFLAFVVMHFWLVGYGLLARSP
                     ELPTITHDAINQVRTGINTVGAWGLAIVFFRAYSMGAGTYTGIEAVSNGLPILREPRT
                     VTGKRTMLYMAISLAGLAGGILVGYLLFNVAPVKGQTLNAVLFEHVAGNWQFLGLPVG
                     VPIVTFTLMTEGALLFVAAQTGFIDGPRVLATMATDRWLPRRFAQLSSRLVTQDGVLA
                     MGLAAIAILVFTRARVDLLVVLYAINVFVTFTLSQLGMSVHWWQERKREPHWGRKLAI
                     NGIGCTFTALILLLTITLKFDEGGWVTVVITGALIALCYAVRHHYRYIAQAIEQLEAD
                     ILPEIFAAKESPPEKYDPTAPTAVLLVNGFNGLGLATLLTLRRLFRDQYRNVIFVGVG
                     EVEASRMKGPEEVRQLEQQVADDLSEYCRFATDLGLHSELRVSIGPDVIAELRRLCLD
                     VAHEFPQAVFFAGKLIFEAELESFISRFLHNHTALELQKFLQVHGLSLVILPVRVMPS
                     AVRRETEMAKRQVA"
BASE COUNT          514 a          773 c          747 g          412 t
ORIGIN      
        1 tcgatgtctc tcccaggcgc gaggcgcgct ggcgtgcaag ggtgctgtag caagcggcgc
       61 gcgtgcttag caactccgcg tgcgagggaa gaagaatgcg cgcgcgctag gccacctgac
      121 gcttggccat ctccgtttct cgccgcaccg ccgacggcat cacccgcacc ggcaggatca
      181 ccaggctcag cccatgcact tgcaggaact tctgcaactc gagcgcggtg tgattgtgca
      241 ggaagcggct gatgaagctt tccagttcgg cctcgaaaat tagtttgccg gcgaagaaca
      301 ccgcttgcgg gaattcatgc gcgacatcca ggcataggcg gcgcaactcc gcgatgacat
      361 cggggccgat actgacgcgc agttcggaat gcagcccaag gtcggtggcg aagcggcagt
      421 actcgctcag atcgtcggcc acctgttgct caagctgcct gacctcttcg ggacccttca
      481 tccgcgacgc ttcgacttcg cccacgccga cgaagatcac gttgcggtac tggtcgcgga
      541 aaagccggcg cagcgtcagc agggtcgcga gacccaggcc gttgaaaccg ttgaccagca
      601 gcaccgcggt cggcgcggtg gggtcatact tttcaggcgg actctccttg gcggcgaaaa
      661 tctcgggcag gatgtcggcc tcgagctgtt cgatcgcctg ggcaatataa cggtaatggt
      721 gacgcaccgc gtagcagagc gcgatcaggg cgccagttat cacgaccgtc acccagccgc
      781 cctcgtcgaa cttcagcgtg atcgtcagca gcaggatgag cgccgtgaac gtgcagccta
      841 tgccgttgat cgcgagcttg cgaccccagt gcggctcacg cttgcgctcc tgccaccaat
      901 gcacgctcat cccgagctgc gagagcgtga acgtgacgaa cacgttgatc gcgtagagca
      961 cgaccagcaa atccacccgc gcccgcgtga acaccagaat cgcgatcgcc gccagcccca
     1021 tcgcaagcac cccgtcctgg gtcaccaggc gcgagctgag ctgcgcaaag cgccgcggca
     1081 gccagcggtc ggtcgccatc gtagccagca cgcgcggtcc gtcgatgaaa ccggtctgcg
     1141 cagcgacgaa cagcagggcg ccctctgtca tcagggtgaa ggtgacgatt ggcactccaa
     1201 ccggcaggcc taaaaactgc cagttgccgg ccacgtgttc gaacagcacc gcgttgagcg
     1261 tctggccctt taccggcgcg acgttgaaca gcaaatagcc gaccaggatg ccgccggcca
     1321 gtccggccag tgagatcgcc atgtagagca tcgtgcgctt gccggtcacc gtgcgcggtt
     1381 cccgcaagat cggcagaccg ttgctgaccg cctcgatgcc ggtgtaggtg ccggcgccca
     1441 tgctgtaggc acggaagaag acgattgcca gtccccacgc gcctaccgtg ttgataccgg
     1501 tacgcacctg gttgattgcg tcgtgagtga tcgtcggtag ttccgggctc ctggccaaaa
     1561 gcccatagcc gaccagccag aaatgcatca ccacgaaagc caggaagatc ggcagcagcg
     1621 agagcaccga ttccttgatc ccccgcaggt tcattccgac catcaccacg accaccaaaa
     1681 ggcacaccca gaatctcagc caatgcaaat gcgttggcag aaagctgaag atggcgtcgc
     1741 cgccgcttgc gatggagatc gaaatggtca agacgtagtc gaccaccagc gcacatcctg
     1801 acaccagccc gggatagggc cccaacagct tggtcgccac caggtagccg ccgccgcccg
     1861 acggaaacaa gtcgatcgtt tgcgagtacg aggccgagat gatgaagacg gtcagcgccg
     1921 tcatcacggc caggaacacc gccaggtact ggtcgtggcc gagcgcgagg aaagcctcct
     1981 cgggaccgta acacgacgaa ctcagtccgt cggagccgag accgacccaa gccaggaagg
     2041 cgactaatga gaggctatgg aatattccgg gatcgcgaac gtcgcgcggc ggtccgatga
     2101 agaggtcggt gagggttcgt ttgccgccgc catttggggc gtcgctgagg gtgcgcttgg
     2161 tgccacctgg accgtcgcta ccgggttgtg atgccatcgg atttatatct gagaccttct
     2221 catattaggg agagcagttt agacacaaac cccgacgggg gccaattgga gtctaatccg
     2281 ttagaatcgt ggctgaatca aacgaatacg tggcacgctt caagcccaga ctgtaacgcg
     2341 ctgcgtcgat cagattctgc atcagcgcga gcacggtcat cggaccggtg ccgcccggat
     2401 taagcgtcag ccatccggcg acttccttca cctgctcgaa gtcgac
//
LOCUS       DATMRM010000175         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3497173, whole genome shotgun sequence.
ACCESSION   DATMRM010000175 DATMRM010000000
VERSION     DATMRM010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3497173"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VNZ06_05105"
     CDS             <1..337
                     /locus_tag="VNZ06_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWW20162.1"
                     /translation="QKIALAQYEKAIQSGFRDVADALALTASIDRQHRAQQGLVEATS
                     QAYELSQQRYKTGADSYLTLLDAQQRYYAAQQGLISTRLAEQSNRVTLYKALGGGWQE
                     HSGQTASNH"
     gene            312..941
                     /locus_tag="VNZ06_05110"
     CDS             312..941
                     /locus_tag="VNZ06_05110"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWW20163.1"
                     /translation="MAKPLRIIEARGNGLAARAAHQRERILDAAEKCFIVAGFHAAGM
                     ARVAETAGVSAGLIYRYFDSKASIVKAIVARHLENEGSRVIDQLASPEEMCDAMLDLF
                     DRWRRGDDPKMNACLLLDLAAECSRDSKIARVVRDKDRMVEEHLARAVERIAEARGTR
                     VGAAEARSRAIILQCLIEGLASRAVRDPTLQRRTLKSAMEKIVTVLLQG"
     gene            1140..>2205
                     /locus_tag="VNZ06_05115"
     CDS             1140..>2205
                     /locus_tag="VNZ06_05115"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWW20164.1"
                     /translation="MLEQWPCAVLLFDADARLHWCNDAARQLSTSRRESLLGLGAPGL
                     GLAWALAPEQFVAALSGTELKFRAGPTDAPHRYDIRLRRVDVGGARHAVMCLIEPHLG
                     ASEDAIGSEREQSFDAAQAGIWRWNLASGEASVDESWCHHLQLNPCSGGEHPTRWALQ
                     VHPDDGPDYRRRLSELKSGATAYFEDEYRILTLDNRWLWILQRGRVIARNADGTPQRV
                     IGICIDIDRRKREETASKANEARLATALWGARAAFWQWHLPTDIRTMSPMWYAMTRYT
                     REQWETLPNPWLSRLHDDDREAVIHAVDEYRKGSVDSLEYEYRLRVSSGEWKWMLDRA
                     RAVEWDLDGNPTVIMGVSLDI"
BASE COUNT          413 a          669 c          740 g          383 t
ORIGIN      
        1 gcagaagatc gcgctggcgc agtacgagaa ggcgatccag tccggttttc gcgacgtcgc
       61 cgacgcgttg gcgctcaccg cgtcaatcga tcgccagcat cgtgcacagc aggggctggt
      121 tgaggcgact tctcaggcgt acgagctttc gcagcagcgg tacaagacgg gcgcggacag
      181 ctatctcacc ctgctcgatg cgcaacaacg ctattacgcc gcgcagcaag ggctgatttc
      241 gacccggctg gcggagcaga gcaatcgcgt gacgctctac aaagcactag gtggaggctg
      301 gcaggagcac agtggccaaa ccgcttcgaa tcattgaggc gcgcggcaac ggactcgccg
      361 cgcgcgctgc ccatcagcgc gagcgcatcc tggatgcggc cgagaaatgt ttcatcgttg
      421 ccggctttca tgcggcaggg atggcgcggg tggcggaaac cgctggcgtg agcgccgggc
      481 tgatctaccg ctacttcgac agcaaagcct cgatcgtcaa agccattgtc gcgcggcatc
      541 tggagaacga gggctcgcgg gtgatcgacc agctcgcatc gccggaggaa atgtgcgatg
      601 ccatgctcga tctgttcgac cgctggcgtc gtggcgatga cccaaagatg aatgcctgct
      661 tgctgctgga tctggccgcc gaatgcagcc gcgattcgaa gatcgcgcgt gtggtgcgtg
      721 acaaggaccg catggtcgag gagcatctgg cgcgcgcggt cgagcgcatc gccgaggcac
      781 gtggcacccg ggtgggcgcg gcagaggcgc gcagccgggc cattattctt cagtgcttaa
      841 tcgagggact ggcgtcccgg gccgtgcgcg atccgacctt gcaacgccgt accctgaagt
      901 cggcgatgga gaagattgtc acggttctgc tacagggctg agcattgcgc ctgaaaccgt
      961 gacccagcac gcgccccggg gggtccggac ctgtcgggct ttgttaattc cgtcgcattt
     1021 cgctttgcag cgcaggccca caatgccgtc agaaatgaac acggcaggcc atgggcgaaa
     1081 ccgccataac gcgtgaactc cgggccccta ccgccccgga aaccaagcct ctgcttgcga
     1141 tgctggagca gtggccgtgc gcggtactgc tgtttgatgc ggatgcgcga ctgcactggt
     1201 gcaacgatgc ggcacgacag ctttcgacct cgcgccgcga gtcactgctg gggctgggtg
     1261 ccccggggct cgggctggcc tgggcgctgg cgcccgaaca attcgttgcg gccctgtccg
     1321 gcaccgaact caaatttcgg gcgggaccca cagacgctcc ccatcgttac gacatacgtc
     1381 tgcggcgagt ggatgtcgga ggcgcacgcc atgcggtgat gtgcctcatc gagccgcacc
     1441 tcggcgcgag cgaggatgcc atcggcagcg aacgcgagca gtcgttcgat gctgcacagg
     1501 ccggtatctg gcgctggaat ctggccagcg gagaggcctc ggtcgatgag tcctggtgcc
     1561 atcatttgca actcaatccc tgctccggtg gcgagcatcc gacacgctgg gcattgcagg
     1621 tacatccgga cgacggcccc gactatcgcc ggcggctgtc agaactgaag agcggtgcga
     1681 ccgcatactt cgaggatgag taccggattc tgacgctaga caaccgctgg ctctggattc
     1741 tgcagcgcgg tcgcgtgatc gcacgcaacg cggacggcac gccgcagcga gtcatcggca
     1801 tttgtatcga tatcgatcgg cgcaaacgcg aggagaccgc gtccaaggcc aatgaggcgc
     1861 ggctcgcgac agcgctgtgg ggtgcgcgcg ccgcgttctg gcaatggcat ctgccgaccg
     1921 acattcgcac gatgagcccc atgtggtatg caatgacgcg ctacacgcgc gaacagtggg
     1981 agacgcttcc caatccgtgg ctgtcgcgcc tgcatgacga tgatcgtgag gccgtgattc
     2041 atgccgtgga tgaatatcgc aagggcagcg tggacagcct cgaatacgaa taccgcctca
     2101 gagtgtcgag cggcgagtgg aaatggatgc tcgatcgcgc ccgcgcggtg gaatgggacc
     2161 tggacggcaa tcccacggtg atcatgggcg tctcgctcga catcg
//
LOCUS       DATMRM010000176        17284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_88061, whole genome shotgun sequence.
ACCESSION   DATMRM010000176 DATMRM010000000
VERSION     DATMRM010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17284
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88061"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..3929
                     /locus_tag="VNZ06_05120"
     CDS             171..3929
                     /locus_tag="VNZ06_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HWW20165.1"
                     /translation="MNTYGLRIAAPLLLLGCTVAAVSFAAGGMEHAVSRQYVTAPAAP
                     NAQSPEAAAAKSSGCLSCHTQTDSPSMHVSSAVNLGCVDCHGGNNSVVLPSGATAGSA
                     QYRKTQDSAHVLPRYPQAWHYPSSANPKQSYTLLNRESPEFIRFENPSDFRVARESCG
                     ACHADIIEASIRSMHATDVMLLGGAAYNNGILDAKNYILGESYTRDGVGATLKGPVNL
                     DPTVAFNASILPVLYPLPSWETLKPGDIFRIFERGGRNIGNLFSETGLPDANGNIERI
                     EEPGRPDIRASNRGAGTGARISVPVINITKTRLNDPLLWFMGTNDQPGDYRQSGCASC
                     HVVYANDADAYHSSVYAQFGHDGTSQTIDPTISKTESGHPVRHAFARSVPTSQCMVCH
                     MHQPNMFLNSMLGYTMWDYESDAPSMWPAKQQYPSIKQQREILDRNPEGAAVRGKWAD
                     VNFLRSVARLNPTLKDTQFADYHGHGWNFRAVFKRDRAGDLLDAKGQIVDDNDPQKFQ
                     KAVHLQSAHVDVGMQCVDCHFSQDNHGNGYIYGEVAAAVEIGCKDCHGTVKYYPNLHT
                     SGPAALEGGKDLSALRTPDGRRRFEWVNGELYQRSMIDPKLEWRVSLVKDTVTPGNAH
                     YNEKAARAKLMSTDVATLSWGAGVPEQKLAHSDSSMECYACHTSWTTSCGGCHLPIEA
                     NAKTERHHYEGGVTRNYATYNPQVARDDMFLLGHRESAKGGRIAPYRSSSALVLSSTN
                     ANREHVYVQQPPISASGYSSQAFNPHYPHTERKVETKTCADCHLSKANDNNAIMAQLL
                     MFGTNFINFVGYHAYVGDKGAVSAVQVTEWDEPQAVIGSYLHKYAYPDYYAQHLKNGR
                     ALQHAQEHEAGDAECIQLRGEYLFVAEGSRGMRVYDVASASNKGFSEGVITAPVSPLG
                     QNTRIRSRNATCVALPTNQPVNPARNTPAKMRTENQEMVMHPLYHYAYITDAEEGLIL
                     TNVDTLVDGEARNNFFSRALTWNPDGILKGARHITIGGSQLYITTPRGLVIVSVDNPL
                     TPKVIAQLPLPDPRATALQFRYLFVTDAKGLEVVDVTHPDKPRLLPNNVIALNDAHRV
                     YVARTYAYVAAGREGLVIVNAERPEALREYQRFNAGGALSDARDVVVASTNASLYAYV
                     ADGSGGLKVLQLTSPESQPKFYGFSPEPKPQLIASFATERPAMSLSKGLDRDRAVDES
                     GNEIAVFGRRGARPLSLDEMKALYLDASGNPWFVPDDTPPVHTAANSR"
     gene            3936..5243
                     /locus_tag="VNZ06_05125"
     CDS             3936..5243
                     /locus_tag="VNZ06_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HWW20166.1"
                     /translation="MMRAYSILCCALFFAVPPWTLARAADTPPATAGAYKHLGVASCA
                     TSVCHGKETEQTGRDVALNEYRIWLEQDRHSQAYRALDNARSRQIAANLGLPSATTAK
                     LCLDCHADNVPATQRGPKFQISDGVQCEACHGGSEKWIETHSQQSVTHAANVANGLNP
                     TEQPLRRAQVCLSCHEGTADQFTTHRIMGAGHPRLAFELDTYTYNQPRHFKADTPSYV
                     KRKGKIPETNLWITGQLQTTQRYLTLVQSPLFTPGGLTPELSMFDCFACHHSIDKPHW
                     TRAHAGNGIPPGTLRLQRQYFLVLQAITETLEPKLTQELVAATDTLMQAGQTDAGKAR
                     AAAKALQDWVRARDEWSKRSYSANDTAALRKALLRFAAEDRASDFAMAEQVVLGVESL
                     SYSLGDHERRKSAIDALYNGVASAAKFDPAQFMQIARGLQGQF"
     gene            5248..6066
                     /locus_tag="VNZ06_05130"
     CDS             5248..6066
                     /locus_tag="VNZ06_05130"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HWW20167.1"
                     /translation="MTEFAALTHPGNREGENQDSIGWDTQQQLWFVADGMGGHASGEV
                     ASRVVKETLLAQAALVGVPAAVLLAHEAVCREAQQLDPARGMGSTVVTAQIQNRSAQI
                     VWVGDSRAYLWRRGGLQRLTRDHSYLEMLRQEENLSETALRNQQGSNVVTQALGMGTP
                     DPSQANVPLRHGDWIVLCSDGLPVELRDSEIAKTLAAHSASPKEAADALLKATMEHGG
                     RDNISIIVIPYDGVSARGTWSLPGFKSRIFEWTAVLAGMVLAAAIALAWKRWTK"
     gene            6070..7026
                     /locus_tag="VNZ06_05135"
     CDS             6070..7026
                     /locus_tag="VNZ06_05135"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWW20168.1"
                     /translation="MAEVQYFLKKADSGKETEIKGNLSVGRSQESGLRLTEGNPSRNH
                     ARLSLTDGVLFVEDLKSTNGTFVNGTKIESRTRLASNDKVRFDVEEYVLRVDVPAPPP
                     DADRTAMRPQALVAESAKPNLPPAWADAGESPGGGKTVFKTMEQMEEERKRARERAAK
                     AGELGPVSVPQLTIYDGGGLPLTVQLRGSDPNNCEWSVGSDEGREIRIQRDGVSGLHA
                     KIVNSEKVWKVVDQVSGNGTFVNGKRVTVSYLKNGDRIAFGPAECVFQAPDTRTAPTP
                     RPSASAASAAPMDARRKRGLLIAGLSFLGTLLLLVVLLKWLG"
     gene            7044..7844
                     /locus_tag="VNZ06_05140"
     CDS             7044..7844
                     /locus_tag="VNZ06_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20169.1"
                     /translation="MAFTRRDLFWYCAGVLTVAVIGLLVRALNGPQNNTPVDLDAHSR
                     VGVMTTPDASDAAASRTEPEPAAASNPRAAGSMDQMLARLEARLAAQGGSDADWELLA
                     QTYQFVGRTDDAKLARSHQLPPSAMSSAAAARSPASDSDRGSASGPAPGPVAISGHVE
                     LAAALRSKVPDGLTLFIVAKSVDSPGPPVAILRTATGHWPLAFTLDDSNAMMPGRTLS
                     SARHVTIEARVSRTGVATPQPGDCQSPIATIDAHQGTPVRIIIDHVIG"
     gene            7860..10547
                     /locus_tag="VNZ06_05145"
     CDS             7860..10547
                     /locus_tag="VNZ06_05145"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20170.1"
                     /translation="MPGPTVEQQRLAASFPEGEGWKKWGPYLAERQWATVREDYSADG
                     NAWEYFPHEHARSRAYRWGEDGLAGISDATQRLCLALAIWNGRDPILKERLFGLTSKQ
                     GNHGEDVKEIYYYLDATPTHSYLKMLYKYPQGEYPYQQLADENRRRSAREPEFELIDT
                     GLFDDNRYFDVFVEYAQNEPDDILMRVRAVNRGHRSAVLHLIPQIWFRNTWSWVTSPA
                     LPVMYRADRHVIGIDHPELGSYQLYAEGEPTLMFCNNESNPRRLWGDQNAQGYWKDAF
                     HDAVIRRHSAALNPREVGTKAGLAYRFDIAAQGSAQVQLRLSKLPQRAPFKQFEAVMA
                     TRLREADEFYAQLQDTLKDSDARLVQRQAFAGMIWSKQFYCFDVYKWLKGDPTQPRPD
                     AARKRNREWEHVENADIISMPDKWEYPWYAAWDLAFHCIPLAHLDTQFAKEQLLLLVR
                     DCYMHPNGQIPAYEWAFSDVNPPVHAWAAWRVFQIDRKTRGSGDYSFLEPIFHKLMLN
                     FTWWVNRKDAAGRNVFQGGFLGLDNIGVFDRSSQLPTGGYLDQADATSWMAMYSLNLM
                     RIALELARHNHVYEDIAAKFFEHFLHIAKAMNNIADRGIGLWNERDEFFYDVLHLPDG
                     AIEPMAVRSVVGLIPLFAVETLEPELLASLPVFKRRVESVLAKRPDLAGLVSHWHDEG
                     RGNRRLLSLLRGRRMKRLLKRLLDEREFLSTYGVRAMSRVHAQQPYVFRHDGMELTVA
                     YQPAESDSGLFGGNSNWRGPIWFPINYLIIESLEKFHHYYGDDFKVECPTGSGRFMTL
                     DEVANELATRLTRIFLKDEYGLRAVNGRPRRQLDPHFADDVLFYEYFDGDTGRGLGAS
                     HQTGWTGLVARLLMPRYLRRAPKSFDLADVTVFNRRPSL"
     gene            10610..12508
                     /locus_tag="VNZ06_05150"
     CDS             10610..12508
                     /locus_tag="VNZ06_05150"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWW20171.1"
                     /translation="MRQRRRDLVRTTLAGGCAAVLAACGSTTNISQSGVLQIPSATEA
                     LSISDVQQIFAQATAEAQARNKPATIAVVDRVGNVLGIFQTTGAAGSTFTINGGRGAV
                     GGLEGIKVLPSEAAAIAKALTAAYFSSNGNAFSSRTAGQIIQQNFDPGQTGATAGPLF
                     NVQFSQLTCSDVSKQASAGTQGPHRSPLGFAAQPGGLPLYKNGTVVGAVGVIADGVYS
                     LVTDYVNNTSPDVNELIAVAGTAGFQAPTGIRADHITAGGNTLIFDTSEALMTNPASA
                     PSFASIIGTAGALDNFAGFGGNPIVAGQAYGTPGSGVVPDTNPALDGTGDYVVVDGTG
                     TNRFPPKAGTDGLMTQAEVIQIIKSTLQVANHTRAQVRQPLGSIAQVSVTVVDTAGEI
                     VGYARSPDALVDSIDVVQQKARTALFFSSPTAAAQLQTLPAANYLDPTATTVLSTSPI
                     TAYVTAAQSFFADPQAFANGIAFSTRAIDNIATPFFPDGIPDTPNGPFSKPIANFSIF
                     NDGLELDLVINKFVAALQGNTTPNCTGLSQIRNGITTFGGGFPIYRGTQLVGAIGVSG
                     DGTDQSDLIGFIGLSEAGKALNTGIGNAPPAMRADTLTPLGTRLRYVNCPQSPYVDSD
                     AQGVCQGL"
     gene            12684..13019
                     /locus_tag="VNZ06_05155"
     CDS             12684..13019
                     /locus_tag="VNZ06_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20172.1"
                     /translation="MHKYKFIVLTGPVPGQEKEYNDWYQNVHLKDVVAIDAFKTAQRF
                     RLQQAVMPVPGLPSYLAIYDIETDDIDGAVKELVDRAASGQMVISKALATDVGFAAVY
                     EQLGPLVKA"
     gene            complement(13154..13465)
                     /locus_tag="VNZ06_05160"
     CDS             complement(13154..13465)
                     /locus_tag="VNZ06_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20173.1"
                     /translation="MRAFIVATLLAAPLCFGAALAQTSAPAAAAHYTTAGTNIGTLLD
                     DPDARAVIDKHIPGFSSQAQIDMARSLTLKGIQQYAPDKYTDQVLADIDADLAKLPAK
                     K"
     gene            complement(13509..14930)
                     /locus_tag="VNZ06_05165"
     CDS             complement(13509..14930)
                     /locus_tag="VNZ06_05165"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HWW20174.1"
                     /translation="MTGLDLLPLHSGPPQVLSSIRLYGFRRMFLRARQSQPSTHLPRG
                     ALVRRCRGRCKMEAFMHGGMLARRRWPQSGDPGYRSQRRATRLQAIFARTQMSPGNKK
                     RSLAVVSVAASLVAEIAVTADMPAIAHAPPVKDDGTITVPSFDLPFSSFASREARDAL
                     VQRLRNPMPMVADIAKTRSMMDERMAPVLERAKSLYPYTVSKSTMGGVPVQTYVPTAG
                     IAPQNKDRVLIELHGGAFVVGGGGPGGASESIPIAGVGHIKVVAVDYRLGPENHFPAA
                     SEDVAAVYRELLKTYKPQNVGIYGCSAGGMLSAETIPWFLKEKLPLPGAIGVFCASLY
                     TFGEGDSAQIQPRLGSVIPIIPPTVPQDNFGASTAYFGGASSKDPLAVPGASKQVMKA
                     FPPTLFLTGTRAPDMSAAAQSHLKLKQLGVKSELLLFDGMDHGFYADPTLPESVTAYE
                     LIAAFFAENLGVKRGKDVKVASR"
     gene            14961..16157
                     /locus_tag="VNZ06_05170"
     CDS             14961..16157
                     /locus_tag="VNZ06_05170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW20175.1"
                     /translation="MCAIAFLSYNLSLACLYGTFSVLLSAVEAKFGATRDLSSLGIPL
                     SMLGIAMTSPFAGALAGKVSIRLLMLTGASMNLLAYVALALSTSIYLNLAVYALLIGP
                     GLCLTATVLPPTLVTRWYAVHRGRALGLITMPIAIAIVPPVAVLMLRGYGLSATYGLM
                     ALIMALLLVPLCFVIDYPPSLIDLSGENAAAETSADPGMSVAQLMRNGRFWLLSLAFA
                     AITTAAIFQGAHLMPMVADWGINATEGSTLLSAAALAGLAGTTTFGWLADRLGGARTL
                     ALVCFNSGVLWAIMLLHPSYPMTLIVVALLGLHGAAVVATVGLALSQQFGQATFGRAF
                     GLANLMGLPFNVLGVPIGARIYMRTGSYSSAIVGIIGFFVLMAVLAATIRSPKTAPAV
                     GLNRSR"
     gene            complement(16247..16438)
                     /locus_tag="VNZ06_05175"
     CDS             complement(16247..16438)
                     /locus_tag="VNZ06_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HWW20176.1"
                     /translation="MYLYRGGGFIGLLILIADIYAIVNIAQSNADTGRKVLWIVIVLV
                     FPFLGWLLWLLAGPRTGRS"
     gene            complement(16460..>17284)
                     /locus_tag="VNZ06_05180"
     CDS             complement(16460..>17284)
                     /locus_tag="VNZ06_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20177.1"
                     /translation="PEIEAERKKLVSKLLTALGQSLNAAPSGLGSGLPATSGNRGAYF
                     KAGEVLASSGKAGPSEIGYSYDAEALCYLRLIPLPPLERPLALANLRKVAERAPLLSR
                     QPEGALVGRNDYGAIAFEPSSPPSRGPGNLASSTQLFITGEIWSIGNSLIARERGERP
                     QWIKLPFLSSVIFERVYYDQLRSLTQFALEHLSLSAPWEVECGVAGSKGLHLWVSAQE
                     TSGPIVQSDVVTRRTMKAGSEAEMDTILLEFFNLLHAAAGSERPKSLHGFPPGRPR"
BASE COUNT         3237 a         5628 c         5384 g         3035 t
ORIGIN      
        1 ggacaggatg tggatatctc gctgatctac cggccgctcg acacgcagaa catcgtgctg
       61 cggacctcgt acgcccagct gttcaccggc ggcgcgtccg aggcactgtt cccgaaaaag
      121 aatccgggtt acttcctctt caatctcatc ctgacgtact agtgaaagcg atgaacacgt
      181 atggattgcg aatcgccgcg ccgctcctgc tgctcggatg caccgtagcg gctgtcagtt
      241 tcgcggccgg cggcatggag cacgcggtct cgcgccagta cgtcacggcg cccgccgcgc
      301 cgaacgcgca gtcgccggaa gcggccgcgg ccaagagcag cggctgtctg tcctgccaca
      361 cgcagaccga cagtccgagc atgcacgtga gcagcgcggt gaacctgggg tgcgtggact
      421 gccacggcgg caacaattcc gtcgtgctgc cgagcggtgc gaccgccggt tcggcgcagt
      481 accgcaagac gcaggacagc gcgcacgtgt tgccgcgcta cccgcaggcc tggcactacc
      541 cctccagcgc caacccgaag cagagctaca ccctgctcaa ccgcgagagt cccgagttca
      601 tccgtttcga gaatccgtcg gacttccgcg tggcgcgcga gtcctgcggc gcctgtcatg
      661 ccgacatcat cgaagcctcg atccgcagca tgcatgcgac cgatgtgatg ctgctgggtg
      721 gcgcggccta caacaacggc atcctcgacg ccaagaacta catccttggc gagtcctata
      781 cgcgcgatgg cgtcggcgcc acgctcaagg gcccggtgaa cctcgatccc acggtggcct
      841 tcaacgccag catcctgccg gtgctctatc cgctgccctc ctgggagacg ctcaagcccg
      901 gcgacatctt ccgcatcttc gagcgcggcg gccgcaacat cggcaacctg ttctcggaga
      961 ccggactgcc ggacgccaac ggcaacatcg agcgcatcga ggagccgggc cggccggaca
     1021 tccgcgcctc caaccgcgga gcggggaccg gtgcgcgtat ttcggtgccg gtgatcaaca
     1081 tcaccaagac gcgcctgaac gatccgctgc tgtggttcat ggggaccaac gatcagcccg
     1141 gggactaccg ccaatccggc tgcgcgtcct gccacgtggt ctacgccaac gatgcggatg
     1201 cctaccattc aagcgtctac gcgcagttcg gtcacgatgg cacctcccag accatcgacc
     1261 cgacgatcag caagaccgag tccggacatc ccgtcaggca cgcgtttgcg cgttcggtgc
     1321 ccaccagcca gtgcatggtc tgccacatgc accagcccaa catgttcctc aacagcatgc
     1381 tcggctacac catgtgggac tacgagtcgg acgcaccctc gatgtggccg gcgaaacagc
     1441 agtatccgtc gatcaagcag cagcgcgaga tcctggatcg caatccggaa ggcgccgcgg
     1501 tgcgcggcaa gtgggccgac gtcaacttcc tgcgcagcgt cgcgcgcctc aatccgacgc
     1561 tgaaggacac gcagttcgct gactatcacg gccacggctg gaacttccgc gccgtgttca
     1621 agcgcgaccg cgccggcgac ctgctcgatg cgaagggcca gatcgtcgat gacaacgatc
     1681 cgcagaaatt ccagaaggcg gtgcacctgc agtcggcgca cgtggacgtc ggcatgcagt
     1741 gcgtggactg tcacttctcg caggacaacc acggcaacgg ctatatctac ggtgaggtgg
     1801 ccgcggcggt cgaaatcggc tgcaaggact gccatggcac cgtgaagtac tacccgaacc
     1861 tgcacacgtc cggtcccgcc gcgctcgagg ggggcaagga tctgtcggcg ctgcgcacgc
     1921 ccgacggccg tcgtcgcttc gagtgggtga acggcgagtt gtatcagcgc tcgatgatcg
     1981 atccgaaact cgagtggcgc gtgagcctgg tcaaggacac ggtcacgccc ggcaatgcgc
     2041 actacaacga gaaggcggcg cgcgccaagc tgatgagcac cgacgtcgcc acgctctcct
     2101 ggggcgccgg cgtgcccgag cagaaacttg cgcacagcga cagctcgatg gagtgttacg
     2161 cctgccatac ctcgtggacc acgagctgcg gcggctgtca cctgccgatc gaggccaacg
     2221 ccaagaccga acggcaccac tacgagggcg gcgtcacgcg caactacgcg acctacaatc
     2281 cacaggtcgc gcgcgatgat atgttcctgc tcggccaccg cgagagcgcc aagggcggca
     2341 ggatcgcgcc ctatcgctcc agttccgcgc tggtgctgtc atctaccaac gccaatcgcg
     2401 agcacgtcta cgtacagcag ccgccgatct cggccagcgg ctacagctcg caggccttca
     2461 acccgcacta tccgcacacc gagcgcaagg tcgagaccaa gacctgcgcc gactgccatc
     2521 tgtcgaaggc gaacgacaac aacgccatca tggcgcagct gctgatgttc ggtaccaact
     2581 tcatcaactt cgtgggctat cacgcctatg tgggtgacaa gggtgcggtc agcgcggtac
     2641 aggtgaccga atgggatgag ccccaggcgg tgatcggcag ctatctgcac aagtacgcct
     2701 atccggatta ctacgcgcaa cacctcaaga acggcagagc gctccagcat gcccaggagc
     2761 acgaagccgg cgatgccgag tgcatccagc tgcggggcga gtatctgttc gtggcggagg
     2821 gctcgcgcgg catgcgcgtc tacgacgtcg ccagtgcctc caacaagggc ttctcggaag
     2881 gggtgatcac cgcgccggtc agtccgctgg gtcagaacac gcgcatccgc tcgcgcaatg
     2941 cgacttgtgt cgccctgccg acaaaccagc cggtcaatcc ggcgcgtaat acgcccgcca
     3001 agatgcgcac cgagaaccag gagatggtga tgcacccgct gtatcactat gcgtatatca
     3061 ccgacgccga agaaggcctg atcctcacca acgtggatac gctggtggac ggtgaggcgc
     3121 gcaacaactt cttcagccgc gcactgacct ggaatccgga cggcatcctc aagggcgccc
     3181 gccacatcac gatcggcggc tcgcagctct acatcaccac gccgcgcggc ctggtgatcg
     3241 tcagcgtgga caatccgctg acacccaaag tgatcgcgca gctgccgctg ccggatccgc
     3301 gcgccaccgc gctgcagttc cggtacctgt tcgtgaccga tgccaagggt ctggaagtgg
     3361 tggatgtcac gcacccggac aagccgcggc tgctgccgaa caatgtgatt gcgctgaacg
     3421 atgcgcaccg tgtctatgtg gcgcgcacct acgcctacgt ggccgccggc cgcgagggcc
     3481 tggtgatcgt caatgccgaa cggccggagg cgctgcgcga gtaccagcgc ttcaatgccg
     3541 gcggcgcact cagcgatgcc cgcgatgtcg tggtcgcctc caccaacgcc tcgctctatg
     3601 cctacgtggc cgacggcagc ggcggactga aggtgctgca gctcacctcg cccgaatcgc
     3661 agccgaagtt ctacggcttc agcccggaac ccaagcccca gctgatcgca agctttgcca
     3721 ccgagcggcc ggcgatgagc ctttccaagg ggctggatcg cgatcgggca gtggatgaga
     3781 gcggcaatga gatcgccgtg ttcggaaggc gcggggcccg cccgctgtca ctggatgaaa
     3841 tgaaggcgct gtatctggat gcgagcggca atccgtggtt cgtcccggac gatacgcctc
     3901 ccgtgcacac ggctgccaac agtcgatgac ggcggatgat gcgggcgtac tcgattctct
     3961 gctgcgccct gttcttcgct gtcccgccat ggacgctcgc gcgcgcggct gacaccccgc
     4021 ccgctaccgc tggggcgtat aagcacctgg gggtcgcgag ctgcgccacc agcgtgtgtc
     4081 acggcaagga aaccgagcag accggtcgtg acgtcgcgct gaatgaatac cgcatctggc
     4141 tcgagcagga ccggcattcg caggcctacc gcgcactcga caatgcgcgc tcgcgccaga
     4201 tcgcggcgaa cctcggtctg cccagtgcca ccaccgcgaa gctctgcctg gactgtcatg
     4261 ccgataacgt gccggccacg cagcgcggac ccaagttcca gatcagcgac ggggtgcagt
     4321 gcgaggcctg ccacggcggc tcggagaagt ggatcgagac ccacagccag cagagcgtca
     4381 cgcatgccgc caacgtcgcc aacggcttga atccgaccga acagccgctg cgtcgcgcgc
     4441 aggtgtgtct gtcatgccat gagggtacgg ccgatcagtt caccacccac cgcatcatgg
     4501 gtgccgggca tccgcgcctg gcgttcgaac tggatacgta tacctacaac cagccgcggc
     4561 atttcaaagc cgacacgccc tcctatgtga agcgcaaggg caagattccg gaaaccaatc
     4621 tctggatcac cggacagctg cagacgaccc agcgctacct cacgctggtg caaagcccgc
     4681 tgttcacacc cggcggcctg acccccgagc tgtcgatgtt cgattgtttc gcctgtcatc
     4741 actcgatcga caagcctcac tggactcgcg cgcacgccgg caacggcatc cccccgggca
     4801 cgctgcggct gcagcggcag tactttttgg tgctgcaggc gatcaccgaa acgctcgagc
     4861 cgaagctcac gcaggaactg gtcgccgcga ccgacaccct gatgcaggcc ggccagaccg
     4921 acgccggcaa agcgcgcgcg gccgccaaag cgctgcagga ttgggtgcgg gcgcgtgatg
     4981 aatggtccaa acgcagttac tcggccaacg acaccgcggc cctgcgcaag gcgctgctgc
     5041 gcttcgcggc cgaggatcgt gccagcgact ttgcgatggc cgaacaggtg gtgctcggcg
     5101 tcgaaagcct gagctactca ctcggtgatc atgagcggcg caaatcggcg atcgacgcgc
     5161 tctacaatgg tgtcgccagc gccgcgaagt tcgatcccgc gcaattcatg cagatcgcgc
     5221 gcggcctgca ggggcagttc tagcgccatg actgaattcg ccgcactaac gcatccgggc
     5281 aaccgcgaag gcgagaatca ggactcgatt ggctgggaca cccagcaaca actgtggttc
     5341 gtcgccgatg gcatgggcgg acatgcgagc ggcgaagtcg ccagccgcgt ggtgaaggaa
     5401 accctgctgg cccaggccgc gctggtaggt gtaccggcag ccgtgctgct ggcgcacgaa
     5461 gcggtgtgcc gcgaagccca gcaactggat ccggcgcgcg gcatgggttc gaccgtggtt
     5521 accgcccaga ttcagaatcg ttcagcgcag atcgtgtggg tcggcgacag tcgcgcttac
     5581 ctgtggcgtc gcggcgggct gcagcgcctg acacgcgatc actcctatct ggagatgctg
     5641 cggcaggagg agaacttaag cgagacggca ctgcgcaatc agcagggttc gaacgtggtc
     5701 acgcaggcgc tcggtatggg cacgcccgac ccctcgcagg ccaacgtgcc gctgcgccac
     5761 ggggactgga ttgtcctgtg cagcgatggc ctgccggtgg aattgcgcga ctcggagatc
     5821 gccaagacgc tggccgcgca ctccgcctcg ccgaaggagg ccgccgacgc gctgctgaaa
     5881 gcgaccatgg aacacggcgg acgcgacaat atcagtatca ttgtcatacc gtatgatggc
     5941 gtcagtgcga gggggacgtg gagcctgccc ggcttcaaga gtcgcatctt cgaatggacg
     6001 gcagtgctgg caggaatggt attggccgcg gccatcgcgc tggcctggaa gcgctggact
     6061 aaataactca tggcggaagt acagtatttc ctgaaaaaag cggacagcgg caaagagacc
     6121 gagatcaagg ggaatctgtc cgtcggacgc agtcaggaga gtggccttcg gctcacggag
     6181 ggcaatccgt cgcgcaacca tgcgcggctg agcctgacgg acggtgtgct gttcgtcgaa
     6241 gacctcaaat ccaccaacgg tacgttcgtt aacggcacca agatcgaatc ccggactcgg
     6301 ctggcatcca acgacaaggt gcgcttcgac gtcgaggagt acgtactgcg ggtcgatgtg
     6361 cccgcgccac cgcccgatgc cgaccgcacc gcgatgcggc cgcaggcgct ggtcgcagaa
     6421 tcagccaagc cgaacctgcc tccggcctgg gcggatgcgg gcgagtcccc gggcgggggc
     6481 aagacggtct tcaagaccat ggagcagatg gaggaggagc gcaaacgcgc acgcgagcgc
     6541 gcggccaaag ccggcgaact cggcccggtg agtgtccccc agctgaccat ctatgacggc
     6601 ggtggcctgc cactgaccgt gcagctgcgc ggcagcgatc cgaacaactg cgagtggagc
     6661 gtcgggagcg atgagggacg cgagatccgc attcagcgag acggcgtctc aggtttacac
     6721 gccaagatcg tgaattctga gaaagtttgg aaggtcgttg atcaggtgtc gggcaatggt
     6781 acgttcgtca acggcaagcg cgtgacggtg agctacctca agaacggcga tcgcatcgcc
     6841 ttcggtccgg ccgagtgcgt gttccaggcc cctgacaccc ggaccgctcc cacgccccgc
     6901 ccatccgctt cagccgccag tgccgcgccg atggatgccc gccgcaagcg cgggctgctg
     6961 atcgccggac tgtcgttcct gggcacactg ctgctgctgg tggtgctgct gaagtggctc
     7021 ggctgatctg acgcgatgtg agcatggcct ttacccgccg cgacctgttc tggtactgcg
     7081 ccggagtgct gacggtcgcg gtcatcgggt tgctggtgcg tgcactcaac ggcccacaga
     7141 acaacacgcc ggtagacctg gacgctcaca gccgcgtggg tgtcatgacg acaccggatg
     7201 ccagcgatgc cgctgcctcc cggactgagc ccgagccggc tgcggcttcg aacccccgcg
     7261 ccgccggttc gatggatcag atgctggcac gcctcgaggc aagacttgcg gcgcagggcg
     7321 gcagcgatgc cgactgggag ctgttggctc agacctacca attcgtcggc cgcaccgacg
     7381 atgcgaagct cgcgcgctcg caccagttgc cgccttcggc catgagcagc gcggcggccg
     7441 cgcgctcccc ggctagcgat agcgaccgcg gcagcgcgag cggtccggcg cccggaccgg
     7501 tcgcgatctc ggggcacgtg gagctggcgg cagccctcag gtccaaagta cccgacggac
     7561 tgacgctgtt catcgtcgcc aagtcggtgg actcgcccgg cccgccggtc gcgatcctgc
     7621 gcaccgccac cggacactgg ccgctggcct tcacactcga tgactcgaac gcgatgatgc
     7681 cgggccgcac gctctcgagc gcgcggcacg tgacgatcga ggcgcgcgtc tcgcggaccg
     7741 gcgtcgcaac gccgcagccc ggggactgcc agagcccgat cgcgaccatc gatgcccacc
     7801 agggcacgcc ggtgcgaatc atcatcgatc acgtgatcgg ctgaagcgca cctgaagtca
     7861 tgccaggacc cacggtagag cagcagcgtc tggcggcgag ctttccagag ggcgaaggct
     7921 ggaaaaaatg gggtccgtat ctggccgagc ggcagtgggc cacggttcgc gaggactaca
     7981 gtgccgacgg caatgcgtgg gagtacttcc cccatgagca cgcgcgcagc cgcgcctacc
     8041 gctggggcga ggacggtctg gccggcatct cggatgcgac acagcgcctg tgcctcgcac
     8101 tcgcaatctg gaacggccgc gatccgatcc tgaaagagcg cctgttcggt ctgaccagca
     8161 agcagggcaa tcacggcgag gacgtcaagg agatctacta ctacctggat gccaccccga
     8221 cgcattccta cctgaagatg ctctacaagt atccgcaggg agaatatcct tatcagcagc
     8281 tcgcggatga gaaccgccgc cgcagcgcgc gcgaaccgga attcgagctc atcgacaccg
     8341 gcctgttcga cgacaaccgc tatttcgacg tgttcgtcga gtatgcgcag aacgaaccgg
     8401 acgacatcct gatgcgggtg cgcgcggtca atcgcggcca tcgctccgcg gtgctgcacc
     8461 tgatcccaca gatctggttc cgcaatacct ggagctgggt caccagcccc gccctgccgg
     8521 tgatgtaccg cgcggaccgg cacgtcatcg gcatcgatca cccggagctc ggaagctatc
     8581 agctctacgc cgagggtgag ccgacgctca tgttctgcaa caacgagtcg aaccccagac
     8641 ggctgtgggg cgatcagaac gcgcagggct actggaagga tgcctttcac gatgcggtga
     8701 tccgccgtca cagcgcggcg ctcaacccgc gcgaggtcgg caccaaggcc ggcctcgcct
     8761 atcgtttcga catcgcggcg cagggcagcg cccaggtgca actgcgcctg tcaaagctgc
     8821 cgcagcgcgc gccgttcaag cagttcgagg ccgtcatggc gacgcgcttg cgcgaagcgg
     8881 acgagttcta cgcgcagctg caggacaccc tcaaggacag cgatgcacgg ctggtgcagc
     8941 gccaggcgtt cgccggcatg atctggagca agcagtttta ctgcttcgac gtctacaaat
     9001 ggctcaaggg cgatcccacg cagccgcgcc cggatgccgc gcgcaagcgc aatcgcgaat
     9061 gggagcatgt ggaaaacgcc gacatcatct cgatgcccga caagtgggaa tatccgtggt
     9121 acgccgcgtg ggacctggcc tttcactgca taccgctggc gcacctggat acgcagttcg
     9181 ccaaggagca gctgttgctg ctggtccgcg actgctacat gcaccccaac ggccagatcc
     9241 cggcctacga gtgggccttc agcgatgtga atccgccggt gcacgcctgg gccgcatggc
     9301 gggtgttcca aattgaccgc aagacgcgcg gcagcggcga ctacagcttc ctggagccga
     9361 tctttcacaa gctgatgctc aatttcacct ggtgggtgaa ccgcaaggat gccgccggcc
     9421 gcaacgtgtt ccagggcgga ttcctcgggc tcgacaacat cggcgtgttc gaccgcagct
     9481 cacagctgcc caccggcggg tatctggacc aggccgacgc caccagctgg atggcgatgt
     9541 attcgctgaa cctgatgcgt atcgcgctgg agctggcgcg ccacaatcat gtctacgagg
     9601 acatcgccgc caagttcttc gagcacttcc tgcatatcgc caaagcgatg aacaacatcg
     9661 ccgaccgcgg catcggtctg tggaatgagc gcgatgaatt cttctacgac gtgctgcacc
     9721 tgccggacgg ggccatcgaa ccgatggccg tgcgttcggt ggttggactg attccactgt
     9781 ttgcggtgga gacgctggaa ccggagttgc tggcctcgct cccggttttc aagcggcggg
     9841 tcgagagcgt gctggccaag cgccccgatc tggccggttt ggtctcgcac tggcatgacg
     9901 agggccgcgg caaccgccgg ctgctgtcgc tgctgcgcgg acggcgcatg aaacgcctgc
     9961 tcaagcgcct gctcgatgag cgcgagttcc tgtccactta cggcgtgcgg gcgatgtcac
    10021 gggtgcacgc gcagcagccc tacgtgttcc gtcacgacgg catggagctg acggtcgcct
    10081 atcagccggc cgagtccgac tcgggtctgt ttggcggcaa ctccaactgg cgcggcccga
    10141 tctggtttcc aatcaactac ctgatcatcg aatcgcttga aaagtttcac cattattacg
    10201 gcgatgactt caaggtggag tgcccgaccg gctcgggccg tttcatgacg ctggatgagg
    10261 tcgcgaatga actggcgacg cggctcacgc gcatcttcct caaagacgag tatgggttgc
    10321 gggccgtgaa cggccgcccg cgccggcaac tggacccgca cttcgccgac gacgtactgt
    10381 tttacgaata tttcgacggg gataccggcc gtggactggg tgcctcccat cagaccggat
    10441 ggacgggcct ggtcgcccgg ctgctgatgc cgcgttacct gcggcgtgcg ccgaaatcct
    10501 tcgatctggc ggacgtcacg gtcttcaacc gtcgaccttc cctttgatcc tgcgagtgcg
    10561 cgcttgtaga ctgtggaacg ctaacccgcg caagagaaca ctggtagcaa tgcgtcaaag
    10621 acgacgggat ctggtcagga cgaccctcgc agggggctgc gcggctgtac tggcggcgtg
    10681 cggcagcacc accaatatca gccagtccgg cgtgctgcag attccctctg ccaccgaagc
    10741 gctgtccatc agcgacgtgc aacagatctt tgcgcaggcc accgccgaag cccaggcgcg
    10801 taacaagcct gcaaccattg cggtggtcga tcgtgtgggc aacgtgctcg gcattttcca
    10861 gaccaccggc gcggcgggct ctactttcac gatcaatggc ggtcgcggtg ccgtgggtgg
    10921 cctggagggc atcaaggtac tgccctcgga ggctgccgcg atcgccaagg cgctgacggc
    10981 tgcgtatttc tcttcgaacg gtaacgcgtt cagcagccgc accgccggcc agatcattca
    11041 gcagaacttc gatcccggtc agaccggcgc caccgcgggc ccgttgttca acgtgcagtt
    11101 ctcccagctc acctgctcgg atgtgagcaa acaggccagc gccggcaccc agggcccgca
    11161 ccgctcgccc ctgggctttg cggcgcagcc cggcggattg ccgctgtaca agaacggaac
    11221 ggtcgtcggc gcggtcggcg tgattgccga tggcgtgtat tcgctggtca ccgactacgt
    11281 caacaacacc agccccgacg tcaatgaact gattgcggtg gcaggcaccg cgggcttcca
    11341 ggcgccgacc ggcattcgtg ccgaccacat caccgccgga ggcaatacgc tgatcttcga
    11401 caccagcgaa gcgctgatga ccaacccggc cagtgcaccc tcgttcgcgt cgatcatcgg
    11461 cacggccggc gcactcgaca acttcgccgg gtttggcggc aatccgatcg tcgccggcca
    11521 ggcctacggc acccccggct caggcgtggt gcctgatacc aatccggcgc tggatggcac
    11581 cggcgactac gtggtggtgg acggcaccgg caccaaccga tttccgccga aggcaggcac
    11641 tgacgggctg atgacccagg ccgaggtcat acagatcatc aagagcacgc tgcaggtcgc
    11701 caatcacacc cgagcgcagg tgcgccagcc cctgggttcg attgcgcagg tctccgtgac
    11761 cgtggtcgac accgccgggg agatcgtggg ttacgctcgc tcgcccgatg ccctggtgga
    11821 cagcatcgac gtcgtgcagc agaaagcacg caccgcgctg ttcttctcca gccccaccgc
    11881 agcggcgcag ttgcagaccc tgccggcggc caactatctg gatcccaccg ccaccacagt
    11941 gctgagcacc tcgccgatca cggcctatgt cacggccgcg cagagcttct tcgccgatcc
    12001 gcaggccttc gccaacggca tcgccttctc gacgcgcgcg atcgacaaca ttgcgacgcc
    12061 attctttccc gacggcatcc cggacacgcc gaacgggccg ttttccaaac cgatcgcgaa
    12121 cttcagcatc ttcaacgatg gcctggaact ggatctggtg atcaacaagt tcgtcgcggc
    12181 attgcagggc aataccacgc ccaactgcac cggcctcagc cagatccgca atggcatcac
    12241 gaccttcggt ggcggctttc cgatctatcg cggcacgcaa ctggtcggcg cgatcggagt
    12301 atcgggcgat ggtaccgatc agagcgatct gatcggcttt atcgggctga gcgaagccgg
    12361 caaagcgctc aacaccggca tcggcaatgc gcccccggcc atgcgggccg acacgctgac
    12421 tccactggga acgcgcttgc gctacgtcaa ttgcccgcag tcaccttacg tggactctga
    12481 cgcacaaggc gtgtgtcagg gcttgtagcg cgggggtcta gttagccccg tcgcggcggg
    12541 cgcctgaacg ggaaatcact cgtcggtaca ctcgcacgtc caccgtcggt gccttgagta
    12601 cagctggatg ctcgcgagtc ggtaccccgc agatggtcaa ggccacctct gccgtaccct
    12661 tattcaacca ccggggagca accgtgcaca aatataagtt catcgtactg accggtcccg
    12721 tgccgggaca ggagaaggag tacaacgact ggtatcagaa tgttcacttg aaggacgtgg
    12781 tggcgattga cgccttcaag accgcgcagc gttttcgttt gcagcaggcg gtcatgcccg
    12841 ttcccggtct gccatcgtat cttgccatct acgatattga gacggacgac atcgatggcg
    12901 cggtcaagga gttggtggac cgcgcggcaa gcggccaaat ggttatctca aaggccctgg
    12961 cgacggacgt gggattcgcg gccgtgtacg aacagctggg tcccctcgtg aaggcgtgat
    13021 tgagccgaag acgcctttgt ccacgctgat aggaatcgac cgctcgactg tctacggagc
    13081 cgctcgtttc atcgcaacga ttgactgcat cagctgatgg cagtggccga tgacggcagc
    13141 atcagtgcgc cgactatttc ttcgcaggca actttgcgag gtcagcgtcg atatcggcca
    13201 gcacctggtc cgtatactta tctggcgcgt actgttgaat gcccttaagt gtcaacgacc
    13261 gtgccatgtc gatctgagct tgtgatgaaa accctggtat gtgcttgtcg atcaccgcac
    13321 gggcatccgg atcgtccaac agcgtaccga tgttcgtccc tgctgtggtg tagtgggcgg
    13381 cagccgccgg cgcgctcgtc tgggccaacg cggcaccgaa gcacagcggc gcggcaagta
    13441 gggtggcaac aataaaggca cgcatgattg acctctcgga atatgtcttg gaagtagtgt
    13501 tgtctgactt aacgtgatgc aaccttgacg tccttgccgc gcttcacgcc aagattttcc
    13561 gcgaagaatg cagcgatcag ctcataggcc gtcactgatt ccggaagcgt ggggtcagca
    13621 tagaagccgt ggtccatgcc gtcgaagagc agcagctcgg acttaacgcc caattgcttg
    13681 agcttaagat ggctctgcgc ggcggcgctc atatcgggag ctctggtgcc agtgaggaac
    13741 agcgtcggcg gaaaggcctt catgacttgc ttcgaagccc ctggcacggc cagaggatcc
    13801 ttactgctcg cgcccccgaa atacgccgta ctggcgccga agttatcctg aggtaccgtc
    13861 ggcggaatga tcggaatcac gctacccagg cgcggctgta tctgggcgga gtcgccctct
    13921 ccaaatgtgt acaaggacgc gcagaagacc ccgatcgcac caggcagcgg cagcttctcc
    13981 ttcaggaacc aggggatcgt ttcggcactc aacatgccgc cggctgagca gccgtagatg
    14041 ccaacgttct gaggcttata agtcttgagc agttcgcgat aaacagccgc gacgtcttcg
    14101 ctcgcggcgg gaaagtgatt ttcgggcccc agccgatagt cgaccgccac tactttgatg
    14161 tggcccactc ccgcaattgg aatggattcg ctggcgccgc ccggacctcc gccgcccacg
    14221 acaaacgcgc cgccgtggag ttcgatcagc acacggtctt tgttttgcgg cgcgatcccg
    14281 gccgttggca cataggtctg taccggaaca ccgcccattg tcgacttgct gacggtgtaa
    14341 ggatagagag atttggcacg ctccagcaca ggcgccattc tctcgtccat catactgcga
    14401 gtcttcgcaa tatcggcgac cattggcatg gggttgcgaa gccgttgcac gagcgcgtcg
    14461 cgcgcctcgc gactggcgaa cgaagagaac ggcagatcaa acgacgggac ggtaattgtt
    14521 ccgtcgtcct tgacgggcgg tgcgtgcgcg attgccggca tgtccgcggt gaccgcgatt
    14581 tctgcaacga gacttgcggc tacgctcact actgcgaggc tgcgcttctt gtttcctgga
    14641 ctcatttgtg tcctcgcaaa gattgcctgc aaacgggttg cgcggcgctg cgaacgatag
    14701 ccggggtctc ccgactgtgg ccagcgccgt cttgcgagca taccaccgtg catgaatgct
    14761 tccatcttgc atcggccgcg gcaccggcgc acgagcgctc ccctcggcaa gtgcgtgctt
    14821 ggctgacttt gccgagcgcg tagaaacatt cttcggaatc cgtatagtcg aatcgaactt
    14881 aaaacctggg gaggcccact gtgcaaaggc aacaggtcca ggccggtcaa gggccgacca
    14941 atcaccctct agcgccacgg atgtgtgcga tagcgtttct ttcttacaac ctttctctag
    15001 cctgtttgta cgggaccttc agcgttctct tgagtgcggt cgaagccaag ttcggagcca
    15061 cccgcgacct cagctccctt ggcattccgc tctcgatgtt gggcatcgcg atgacatcgc
    15121 cgttcgccgg cgccttggca ggaaaggtct cgatccggct cctgatgctg acgggcgcat
    15181 ccatgaacct gctcgcctat gtcgcgcttg cgctctcgac gagcatctac ctcaatctcg
    15241 cggtctatgc gttactgatc ggccccgggc tgtgtttgac ggcgaccgtc cttccgccca
    15301 cgctcgtgac tcgttggtac gccgtccacc gcggacgcgc actcgggctc atcacgatgc
    15361 cgatcgcgat cgcgatcgtg ccgccggtcg cagtcttgat gctccgcggg tatggcctgt
    15421 cggcgaccta cggcctgatg gcactcatca tggcattgtt gttggtgcca ctttgttttg
    15481 tcatcgacta cccaccgagc ctcatcgacc tgagcggcga gaatgccgcc gctgaaacct
    15541 ctgccgatcc tggaatgagc gttgcccagt tgatgcgcaa cggccgcttc tggttactct
    15601 cgctcgcgtt cgcagcgatc accactgccg ccatcttcca aggtgctcac ctcatgccga
    15661 tggtcgccga ttggggcatc aatgcaacag agggttcgac cttgctttca gccgcggcgc
    15721 tagccggcct tgcaggcaca accacttttg gctggctcgc cgatcgtctg gggggcgcgc
    15781 gaaccctggc tctggtttgt tttaacagcg gggttttatg ggcgatcatg ctgctgcacc
    15841 cctcctatcc tatgacgctc atcgtcgtgg cgctgctggg cctgcatggc gccgcggtcg
    15901 ttgcgaccgt cggcttggcg ctgtcgcaac agttcggaca ggcaaccttc ggccgcgcct
    15961 tcggattggc caatttgatg gggttgccgt tcaatgttct cggcgtacct atcggcgcac
    16021 gcatttacat gcgcacgggt tcctattcga gtgccatagt cgggatcatc ggattcttcg
    16081 ttctgatggc ggtgcttgcg gccactatcc gaagtccgaa aaccgcgcca gcggtggggt
    16141 tgaatcgctc acgatagtgg ccccgcgtgg gcattcgagc ggcagttcgg aaacgctgtt
    16201 cggcgaccgg ttgcgctgcg tcgtttcgac tcgcggatct cgcctatcag cttcgcccgg
    16261 tcctcggccc cgccagcagc cacagcagcc atccgaggaa cgggaacacc agcacgatca
    16321 cgatccacag gaccttgcgt ccggtgtcgg cattgctctg cgcgatattc acgatggcgt
    16381 agatatcggc aatcagaatg agtagcccga tgaatccacc accgcggtaa agatacatcg
    16441 cgaagccccc gaaacttccc tagcgcggcc ggccgggcgg aaacccgtgc aggctcttcg
    16501 gccgctcgga cccagccgca gcgtgcagca ggttgaagaa ttccagcagt atcgtgtcca
    16561 tctcggcctc gctgccggcc ttcatcgtcc ggcgagtgac gacatcgctc tgaacgatcg
    16621 gaccggaggt ctcctgcgcg gacacccaca gatgcagtcc cttgctgcct gcgaccccgc
    16681 attcgacctc ccacggcgcg ctcagcgaca ggtgttccag tgcgaactgg gtcagcgagc
    16741 gcagctgatc gtagtagaca cgctcgaaga tgaccgacga cagaaacggt agcttgatcc
    16801 actgcggtcg ctcgccgcgt tcgcgcgcaa tcagcgaatt tcctatgctc cagatctccc
    16861 cggtgatgaa cagctgggtc gaggaggcga ggtttccggg tcctcgactc ggcggcgagg
    16921 aaggctcgaa ggcaatcgcg ccatagtcat tgcggccgac gagggctcct tccggctgcc
    16981 ggctcaagag cggtgcgcgc tccgcgacct tgcgcaggtt ggccagcgcc agcggccgct
    17041 ccagcggtgg cagcggaatc aaccgcagat agcacagcgc ttcggcgtcg taggagtaac
    17101 cgatctccga aggaccggcc ttaccgctgc tggcaagcac ttcacccgcc ttgaaatagg
    17161 cgccacgatt gccgctggtg gcgggaaggc cgctgccgag cccgctgggc gccgcattca
    17221 gcgactgccc cagcgccgtc agcagcttcg aaaccagttt cttgcgctcg gcttcgattt
    17281 ccgg
//
LOCUS       DATMRM010000177         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4133326, whole genome shotgun sequence.
ACCESSION   DATMRM010000177 DATMRM010000000
VERSION     DATMRM010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4133326"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VNZ06_05185"
     CDS             complement(<1..1380)
                     /locus_tag="VNZ06_05185"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HWW20178.1"
                     /translation="MQARPHLEAGQDTEQRSGKRASRIARRIEADIIRRGWPIGQSLG
                     SEQALQERYRVSRSVLREAVRLVEHHQVARMRRGPNGGLLVCEPDAAPATHAIIIYLE
                     YLGTTVGDLLDARLLLEPLAASLAAEQIDEAGIDRLRAVLRAEEPRDEFHAALAEQSK
                     NPVLQLFIDILIRLTVRYAEHSRLDSASDALAAIDRRHHEHADIVAAVTAGDSAQAKT
                     LSERHAESVTSWLQDHQPPRRDRKRSGLHREAPQGKLAEVLANAIRDDIATSGRRIGE
                     VFGTEVALLERYRVSRSVLREAVRLLEYHAVAKMRRGPGGGLVVAEPQAQASIDTIAL
                     YLQYRKPRGEDLRLVRDAIEIDNVAKVVKRHDAPEVRAFLDTHRLIFEEASDDPRAAG
                     MAEFLFHTGLAEQAGNALLDLFLRIIVEVFRRHWASTEQPIPSHSDIADVQRAHLRII
                     EAIADGDDSL"
     gene            complement(1409..>2367)
                     /locus_tag="VNZ06_05190"
     CDS             complement(1409..>2367)
                     /locus_tag="VNZ06_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWW20179.1"
                     /translation="GGLLNPVISACRAVSEGHARHVIIYRTIQMLGGTVPVKQDENAP
                     AAPLARMFETPEGAEPPVVGPMDDVGDLVAAQAYSAANWLALTCRRHMELYGTTKEQL
                     GWLAINSRRNAALNPLAVYRDPMTMDDYLCARPVSTPLGLLDCDVPIDGSIALVVSAG
                     DYAKDCPHRAVQVEAVGGTDGAGGWFHRADYPKMASADAAAQMWSRTDLTPADLNLAE
                     LYDGFTFLTFAWLEALGICGDGEAGPFVEGAKRIALDGALPLNTYGGQLSAGRMHGYW
                     LLHETCLQLRGEAGERQVPRRPEVAVAAVGGGPIAGCMLLTC"
BASE COUNT          415 a          826 c          720 g          406 t
ORIGIN      
        1 aaggctgtcg tcgccgtccg cgattgcctc gatgattcgc aggtgagcgc gttgcacgtc
       61 agcgatatcg ctgtggctcg ggatcggttg ctcggtactc gcccaatgcc ggcgaaacac
      121 ctcgacgatg atccgtaaga acaagtccag cagggcatta ccagcctgct cggccagccc
      181 ggtgtggaac agaaactccg ccataccggc cgcgcgcgga tcgtcgcttg cttcctcgaa
      241 gatcagccga tgggtgtcga gaaaggcgcg cacctcgggc gcgtcatggc gtttgacgac
      301 ctttgcgacg ttgtcgatct cgatcgcgtc gcgcaccaac cgcaggtctt caccacgcgg
      361 cttgcggtat tgcaggtaca gcgcgatggt gtcaatgctg gcctgtgcct gcggttccgc
      421 gaccaccagc ccgccgccgg gcccgcgacg catcttcgcc acagcgtgat attccagcag
      481 ccgcaccgcc tctcgaagca ccgaccggct gactcgataa cgttccagca gcgcgacttc
      541 ggtcccgaac acctccccta tccgacggcc gctggtagcg atatcgtccc ggatggcgtt
      601 cgccaacacc tcggcgagct tgccctgcgg cgcctcgcgg tgcagtccgc tccgcttgcg
      661 gtcccggcgg ggcggctggt ggtcttgcag ccacgacgtc accgattccg cgtgccgctc
      721 gctcagcgtc ttggcctgcg ccgaatcgcc cgcggtaacc gcagcgacga tatccgcgtg
      781 ctcatggtgc ctgcggtcga tggccgcgag agcatcactg gcggagtcca gccgcgaatg
      841 ttcggcatac cgaacggtca atcggatcag gatgtcgata aatagctgca gcacaggatt
      901 tttagattgc tccgccagtg ctgcgtggaa ctcgtcgcgc ggttcctcgg cccgcagcac
      961 ggcgcgcagc cggtcgatgc cggcttcatc gatctgttcg gcggcaagcg atgccgccaa
     1021 cggctccagc agcagccggg cgtcaagcag atcaccgacc gtggtgccca ggtactccaa
     1081 atagatgatg atcgcgtgag ttgccggggc ggcgtcgggt tcgcagacca gcagaccacc
     1141 gttgggcccc cgacgcatgc gcgcgacctg gtggtgctcg acgagccgaa cagcttcccg
     1201 cagcactgat ctgctcaccc gataacgctc ctgcagcgcc tgctcagaac ctagtgattg
     1261 gccgatcggc cagccgcgac ggatgatgtc agcctcgatg cgacgggcga tccgcgaagc
     1321 tcgcttgccg gaccgctgct cggtatcctg accggcttcg agatgtggac gagcttgcac
     1381 gctcaattgc acactcgcac agaactactc aacatgtcag tagcatgcat ccggcgatcg
     1441 gaccgccgcc caccgcggcc accgcgactt cgggccggcg gggcacctgc cgctcccccg
     1501 cctcgccgcg caactgcagg caggtctcat gcagcaacca gtagccgtgc atgcggcccg
     1561 ctgaaagctg tccaccatag gtgttcagcg ggagcgcacc gtctagggcg atccgcttgg
     1621 ccccctcgac gaacggaccg gcctcgccgt caccgcagat gcccagagct tccagccagg
     1681 cgaaggtgag gaaggtaaac ccgtcataga gttctgcgag attgagatcg gcgggagtca
     1741 gatcggtccg cgaccacatc tgtgccgccg catccgccga cgccattttc ggatagtccg
     1801 cgcggtgaaa ccaaccgccc gcgccatccg tgcccccgac cgcctccacc tgcaccgccc
     1861 gatgcggaca gtctttggcg tagtcgccgg ccgacaccac cagcgcgatc gacccgtcga
     1921 tcgggacgtc gcaatccagc agccctaacg gtgtggacac cggccgtgcg cacagatagt
     1981 catccatagt catcggatcc cggtacaccg caaggggatt cagcgccgca ttacgcctgc
     2041 tgttgatcgc cagccagccc agctgctcct tggtagttcc gtacagctcc atgtgccggc
     2101 ggcaggtcaa cgccagccag ttcgcggcgg aatacgcttg cgcggcaacc agatccccga
     2161 catcgtccat cgggccgaca accggtggct cggcgccctc gggtgtctcg aacatccggg
     2221 caagcggcgc agcgggagca ttctcgtcct gcttcaccgg gaccgtgcca cccagcattt
     2281 ggatcgtccg gtagatgatc acgtgccgcg catgaccctc ggacaccgca cggcatgccg
     2341 atatcaccgg gttcagcaga ccgccgg
//
LOCUS       DATMRM010000178         9133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4464297, whole genome shotgun sequence.
ACCESSION   DATMRM010000178 DATMRM010000000
VERSION     DATMRM010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4464297"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2213)
                     /gene="fdhF"
                     /locus_tag="VNZ06_05195"
     CDS             complement(<1..2213)
                     /gene="fdhF"
                     /locus_tag="VNZ06_05195"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HWW20180.1"
                     /translation="MQSIAEGDLGTPPSAHARRVSLTIDGLELQVPEGTSVMRAAAIA
                     GTRIPKLCATDQLAAFGSCRLCLVQIDGVKGFPASCTTPVAPGMTVSTQSEQLAKLRR
                     GVMELYISDHPLDCLTCPANGHCELQDMAGAVGLREVRYGYDGANHLDAPKDESNPYF
                     TFDVSKCIVCSRCVRACDEQQGTLALTIQGRGFESKVVASQDESFLDSECVSCGACVQ
                     ACPTATLSERSLIEIGQAEHSIVTTCAYCGVGCSFRAEMRGEQVVRMVPNKDGHANHG
                     HGCVKGRFAIGYATHPDRITHPMIRASIQQPWREVGWEEAISHAACELRRIQGKYGRD
                     SIGGISSSRCTNEETYLVQKLVRAGFGNNNVDTCARVCHSPTGYGLKATLGESAGTQN
                     FDSVMDADVIMVIGANPTDGHPVFASQMKRRLRQGAHLIVIDPRRIDLVRTPHIQAAH
                     HLQLRPGTNVAIVNALAHVIVTEGLVKQPFVSARCEPEAFERWSRFVAEERHSPEATE
                     SISGVPAAEVREAARLYATGGNAAIYYGLGVTEHSQGSTMVMGIANLAMATANIGRPG
                     VGVNPLRGQNNVQGSCDMGSFPHEFTAYRHVGDDQVRASFEQEWGRKLLAEPGLRIPN
                     MFEAALDGEFKALYIQGEDIAQSDPNTQHVTAALSAMECIIVQDLFLNETAKFAHVYL
                     PGSSFLEKDGTFTNAERRVSPVRKVMRSKSGYADWEITQLLSNALGLPMNYRHAS"
     gene            complement(2201..3739)
                     /locus_tag="VNZ06_05200"
     CDS             complement(2201..3739)
                     /locus_tag="VNZ06_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HWW20181.1"
                     /translation="MSATLYLPREATALSLGAETALHAIREEAARRGESIRIIRNGSR
                     GLFWLEPLIEVQTARGRIAYGPVRPGDVPSLFEAGWLQGAEHPLHHGLTESIPYLKKQ
                     QRLTCSRVGVIDPLELDDYGAHAGYTALRAALAMTPAEIVQAVSDSGLRGRGGAAFPT
                     GIKWKTVLEAAGAQKYIVCNADEGDSGTFSDRLLMECDPFCLIEGMTIAGLAVGATRG
                     YIYLRCEYPHAEDVLTHAIASAYARGYLGEDLLGSGRRFDLQLRLGAGAYICGEETSL
                     LESLEGKRGQIRFKPPLPAIAGLFGCPTVVNNVISFASVPIILERGAQFYRDYGMGRS
                     RGTLPLQLAGNIARGGLVELAFGATLRELLYDFGGGTRSGRPIRAVQVGGPLGAYWPE
                     SKFDTPLDYEAFTKEGGMLGHGGVVVFDNTVDMAAQARYAMEFCAIESCGKCTPCRIG
                     SSRGVEVIDRLLAGDQAGTQQTLLRDLCDTMLNGSLCALGGLTPYPVLSALDHFPQDF
                     TCSR"
     gene            complement(3736..4254)
                     /locus_tag="VNZ06_05205"
     CDS             complement(3736..4254)
                     /locus_tag="VNZ06_05205"
                     /inference="COORDINATES: protein motif:HMM:NF004638.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HWW20182.1"
                     /translation="MSKRQRVSIPIRPEPGSDLPAAQRDAVLDAVRLHREAPGPLLPI
                     LHAIQQRLGFIAARLVPLIAHELNLSRAEVHGVISFYHYFRQYAPGRHVIYVCRAEAC
                     QSVGARELESHAKRSLGIDFHGTSADGRYSLEAVYCLGNCACGPSMLIDRELHSHVTP
                     DRFDAITRRGGP"
     gene            complement(4251..5081)
                     /gene="fdhD"
                     /locus_tag="VNZ06_05210"
     CDS             complement(4251..5081)
                     /gene="fdhD"
                     /locus_tag="VNZ06_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWW20183.1"
                     /translation="MAKPGSVVSPKPQGVRARDPLETVTARRQEGEFSQAVSESVAAE
                     VPVAMQYNARPYAVMMATPTDLADFAYGFALTERIIASVSELTLVDQLWTEHGVALEM
                     LIPRQRFLMLSARERNLTGRTGCGLCGTATLEAAIRPVRHVDPQAPAADLQTLREGMR
                     RLATLQPLNDASGAVHAAALLSGQDGFTVREDVGRHNAIDKAVGALMRAGLQPHTLLV
                     TSRASYEVVHKAAEVGCRLVAAISAPTSLALQLAREAGITLVGWTRPPRLTIYCGSLS
                     "
     gene            complement(5141..5992)
                     /gene="fghA"
                     /locus_tag="VNZ06_05215"
     CDS             complement(5141..5992)
                     /gene="fghA"
                     /locus_tag="VNZ06_05215"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002050963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HWW20184.1"
                     /translation="METISEHRCYDGVQGFYRHTSSTIGLPMRFSVYRPPQAERGAVP
                     TLFYLAGLTGNEESFAAKGGAQRLAAELGLMLIGCDTSPRNTGIAGEADDWEFGSGAG
                     FYLNATQAPWSQYFRMYDYVRDELRGLVLASFPASPGQLGIFGHSMGGHGALIIALKN
                     PDLFRSVSAFAPICSPSRCPWGLKAFARYLGEDRSTWAPYDAVELLRSAGRALLPQIL
                     VDQGLADKFLTAGQLLPEQFEAACQSSHQPLRLRRHAGYDHNYYFISTFVGDHLEHHA
                     RILAAAQ"
     gene            6030..6704
                     /locus_tag="VNZ06_05220"
     CDS             6030..6704
                     /locus_tag="VNZ06_05220"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HWW20185.1"
                     /translation="MNIELSAHEARILGCLVEKQITTPEQYPLSLNAITNACNQKSNR
                     DPVLALSEQEVQSVIDTLSRKHLVLERAGFGSRVPKYQQRFCNTEFSMLQFTPQERAI
                     VCELLLRGPQTPGELRSRAARMAEFTDASQVEAALEALRTRESGPMVQRLLREAGRRE
                     SRYAHLFSGAIIETATAEPPAVHVASASSAPSTSLPSSALERLIALEEQVQQLREEVA
                     QLKLKA"
     gene            complement(6705..8012)
                     /locus_tag="VNZ06_05225"
     CDS             complement(6705..8012)
                     /locus_tag="VNZ06_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20186.1"
                     /translation="MDDSSLIRVEAEHLRSSGVLGEARLRRLFDYLVDSTLAGHAPKE
                     ISIAIDVFGKGADFDVSQDALVRVYVHKLRRALADYYRACAGEGRPALQIPKGEYRLR
                     VISAKAAAEIEPVAPEASGRTDRRVWMAYGVAAAGVLALIVGAILAWGAPRSEVEQVR
                     ANPIWSSLLKDDRPIMIVVGDYYLIGETDSMGVKRLIREYTVNSKGDLDHFVAEHPEV
                     ADRYIDVGLRYLPVSSAFALRDVMAVLAPQNRRITISKMSDVEPSSLKADDIIYIGYL
                     SGMGMLQDLVFTPESRFTVGESYDEVIDKKTKHSYASEVGSQVMDPPQPSGKEQAYHD
                     YGVFAKFRGPGGNVVLVISGTRDEGVSQTAETFTSNQRLQEFAHQADVSHPIEALLEV
                     SAFDGVNLSGKLLLESNRDIAAMPATADARAMPPVSSAATQPR"
     gene            8134..8661
                     /locus_tag="VNZ06_05230"
     CDS             8134..8661
                     /locus_tag="VNZ06_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWW20187.1"
                     /translation="MTANSFARIGARVLCAAGVLATNPAIPAGPDARAIPAAGLAISQ
                     LELMRASVQIPADGIWGAAGADKLSDDEWLLAEQDSTNLVAAASLLGMPGTGKKDRNW
                     IASADWQGWVREFRRTALELHAAAKAHDLPKLSAAGDHLTKVCEDCHAKYRPATPSDG
                     VSRYPFYPKRELPSQ"
     gene            8673..>9133
                     /locus_tag="VNZ06_05235"
     CDS             8673..>9133
                     /locus_tag="VNZ06_05235"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWW20188.1"
                     /translation="MAALRTCSPQPGIPRGLISFALRALLGLATAARADDTSSADDQS
                     SSQALEEISVTAQRLQLLGSAESASEGIVADQELQLTPAYRPAQLLETVPGLTVTAHS
                     GEGKANQYLLRGYNLDHGTDLATFVDAMPVNQPTHAHGQGYTDLSFMIPELA"
BASE COUNT         1673 a         3012 c         2894 g         1554 t
ORIGIN      
        1 tcggaggcat gccggtagtt catgggcagc ccgagtgcat tggacagcaa ctgcgtgatc
       61 tcccagtcag cgtaacctga cttggagcgc atcaccttgc gcaccggcga cacgcggcgc
      121 tcagcgttgg tgaaggtgcc gtctttctcc aggaaggacg agccgggcag atacacatgc
      181 gcgaacttgg cggtctcgtt caggaacaga tcctgcacga tgatgcattc catggcgctc
      241 aacgcggcgg tgacgtgctg ggtgttcgga tcggactgcg cgatgtcttc gccctggatg
      301 tagagcgcct tgaactcacc gtccagcgcc gcctcgaaca tgttgggaat gcgcagaccc
      361 ggctcagcca gcagcttgcg gccccattcc tgctcgaagc tcgcgcgtac ctggtcatca
      421 ccgacgtggc ggtaggcggt gaactcgtgc gggaacgagc ccatgtcgca cgagccctgc
      481 acattgttct gaccgcgcag cgggttgacg ccgacacccg ggcgcccaat gttggcggtc
      541 gccatcgcca gattggcaat gcccatcacc atcgtcgagc cctgactgtg ttcggtcaca
      601 ccaagcccgt agtagatcgc ggcgttgccg ccggtcgcgt acaggcgcgc cgcctcgcgc
      661 acctcggccg ccggcacacc gctaatggat tcggtggcct ccggagagtg gcgctcctcg
      721 gcgacgaacc tggaccagcg ctcgaacgcc tccggctcgc aacgcgcact gacaaatggc
      781 tgcttcacca gtccttcggt gacgatcaca tgcgccagcg cattgacgat cgcgacgttg
      841 gtgccaggtc gcagctgcag atggtgcgcg gcctgaatgt gcggtgtgcg caccaggtcg
      901 atgcggcgcg gatcgatgac gatgaggtgc gcaccctggc gcaggcgacg tttcatctgc
      961 gaggcgaaca ccgggtgtcc gtcagtggga ttggcgccga tcaccatgat gacgtcagcg
     1021 tccatcacgg aatcgaagtt ctgcgttccg gccgactcac ccagggtggc cttcagtccg
     1081 tagcccgtgg gtgaatggca cacgcgcgca caggtgtcca cgttgttgtt gccgaagccc
     1141 gcgcgcacca gcttctgcac cagataggtt tcctcgttgg tgcaacggct ggaactaatg
     1201 ccgccgatgc tgtcgcggcc gtacttgcct tgaatgcgac gcagctcgca ggccgcatgc
     1261 gaaatcgcct cctcccagcc cacctcgcgc cagggctgct ggatgctggc gcggatcatc
     1321 gggtgggtga tgcggtccgg atgcgtggca tagccgattg caaagcgtcc tttgacacaa
     1381 ccgtggccgt ggttggcatg cccgtccttg ttggggacca tgcgcacgac ctgctcgcca
     1441 cgcatctccg cgcggaacga gcagccgacc ccgcagtaag cacaggtcgt gacgatgctg
     1501 tgctcggcct ggccgatctc aatcagcgac ctctccgaca gcgtcgcggt gggacaggcc
     1561 tgtacgcagg cgccacagga gacgcactcc gaatccagaa acgactcgtc ctggctggcc
     1621 acgaccttgg actcaaaacc gcggccttga atcgtcaaag caagcgtgcc ctgctgttcg
     1681 tcgcaggcgc gcacgcagcg tgagcacacg atgcacttgc tgacatcgaa agtgaaatag
     1741 ggatttgact catccttcgg tgcatccaga tgattggcgc cgtcatagcc gtagcgcacc
     1801 tcgcgcagtc cgacagcgcc ggccatatcc tgcaactcac agtggccgtt cgccggacag
     1861 gtcagacagt ccagcggatg atcggaaatg tacagctcca tcacgccgcg ccgcagcttg
     1921 gcgagctgct cactctgcgt tgagaccgtc atgcccggcg cgaccggggt cgtacaggag
     1981 gcgggaaacc ctttgacccc atcgatctgc accagacaca gccggcatga tccgaacgcc
     2041 gcgagctgat cggtcgcaca cagcttcggg atccgggtac cggcgatggc cgccgctcgc
     2101 atcaccgagg taccctccgg tacctgcagt tcgaggccat cgatcgtcag gctgacccga
     2161 cgcgcgtgcg cactgggcgg tgtgcccaga tcgccttccg ctatcgactg catgtgaaat
     2221 cctgcggaaa atgatccagc gccgagagta ccggataggg tgtcaggccg ccgagtgcgc
     2281 acagcgagcc gttgagcatc gtgtcacaca gatcgcgcag cagcgtctgc tgcgtcccgg
     2341 cctgatcgcc ggcgagcaac cggtcgatca cttccacgcc acgggacgaa ccgatgcgac
     2401 agggagtgca tttgccgcac gactcgatcg cgcagaactc catggcatag cgcgcctggg
     2461 ctgccatatc gaccgtattg tcgaatacca cgacaccacc gtggccgagc atgccacctt
     2521 ctttggtgaa cgcctcatag tccagaggcg tatcgaattt cgattcaggc caatacgcac
     2581 cgagcgggcc gccgacctgc acggcgcgaa tcggtctgcc gctgcgtgtg ccgccgccaa
     2641 aatcgtaaag cagttcgcgc agcgtggcgc caaaggcaag ttccaccagg ccaccgcgcg
     2701 cgatattacc ggcaagctgc agcggtagcg tgccgcggga gcggcccatg ccatagtcgc
     2761 gatagaactg cgcgccacgc tccagaatga tcggcacgct ggcgaaactg atgacgttat
     2821 tgaccaccgt cgggcagccg aacaggccgg caatggcagg tagcggcggc ttgaagcgga
     2881 tctggccgcg cttgccttcg agcgactcga gcagcgaggt ctcctcgccg cagatatacg
     2941 cacccgcacc cagtcgcagc tgcagatcaa agcggcgtcc cgatcccagc aggtcctcgc
     3001 ccagatagcc gcgtgcgtag gcgctcgcga tggcgtgtgt cagcacatct tcggcatgcg
     3061 gatactcgca gcgcaggtag atatagccgc gcgtcgcgcc gaccgccagg ccggcgatcg
     3121 tcatgccctc gatcagacag aaggggtcgc attccatcag tagtcggtcg gaaaacgtgc
     3181 ccgagtcgcc ttcgtcggcg ttgcagacga tatatttctg cgcaccggcg gcctcgagca
     3241 ccgtcttcca tttgatgccg gtcgggaacg ccgccccgcc gcgcccgcgc agacccgaat
     3301 cgctcactgc ctgcacgatc tcggccggcg tcatcgccag cgcggcgcgc agagcggtgt
     3361 agccggcgtg cgcaccataa tcgtccagtt ccagcggatc gatcaccccg acgcgcgaac
     3421 aggtgagccg ttgctgcttc ttcaggtacg gaatcgactc ggtcagcccg tgatgcaaag
     3481 gatgctccgc gccctggagc catcccgcct cgaacagtga cggcacgtcg ccggggcgta
     3541 ccggaccgta agcgatgcgg ccgcgtgcgg tctgtacctc gatcagcggt tcgagccaga
     3601 aaagtccgcg cgagccattg cggatgatcc tgatcgactc acctcggcgc gcggcctcct
     3661 cgcggatggc atgcaacgcc gtctcggctc cgagcgagag cgcagtcgcc tcacgtggca
     3721 gatagagggt ggcgctcatg ggccgcccct acgggtgatg gcgtcgaagc gatcgggcgt
     3781 gacatgcgag tgcagctcgc ggtcgatcaa catcgacggc ccgcaggcgc aattgccgag
     3841 gcaatacacc gcctcgaggc tgtagcgacc atcagcgctg gtgccgtgga aatcgatgcc
     3901 gagcgagcgc ttggcgtgcg actccagctc gcgcgcgccc accgactgac aggcttccgc
     3961 gcggcacacg tagatcacgt gtctgccggg cgcgtactgg cgaaaataat gatagaagct
     4021 gatgacgcca tgcacctcgg cgcgagacag gttcagctcg tgcgcgatca gcggcaccag
     4081 tcgtgcggcg ataaaaccga gtctttgctg aatcgcatgc aggatgggca gtagcgggcc
     4141 cggcgcctcg cggtgcaaac gcacggcatc gagcaccgcg tcgcgctgcg ccgccggcag
     4201 atcggaaccg ggctccgggc ggatcggaat gctcacgcgc tggcgtttgc tcacgacagg
     4261 ctcccgcaat agatcgtcaa tcgtggcgga cgtgtccagc cgaccagcgt gatcccggct
     4321 tcgcgggcca gctgcagggc cagtgaggtc ggtgccgaga tggcggcgac cagccggcag
     4381 cccacttcgg ccgccttgtg taccacttca taggaggcgc gcgaggtcac cagcagcgtg
     4441 tgcggctgca ggccggcgcg catgagggca ccgaccgcct tgtcgatcgc gttgtgccga
     4501 ccgacatcct cccgcaccgt gaaaccatcc tgccccgaca gcagcgcggc ggcatgaacc
     4561 gcgccgctgg cgtcattcag cggctgcagc gtggcgaggc gccgcatgcc ttcgcgcagg
     4621 gtttgcagat cggccgcggg tgcctgcggg tcgacgtgac gcacggggcg gatggccgcc
     4681 tcgagcgttg ccgttccgca cagtccgcag cccgtacggc cggtgagatt gcgctcgcgc
     4741 gctgagagca tcaggaatcg ctgccgtgga atcagcatct ccagggcgac tccgtgttcg
     4801 gtccagagct gatctacgag tgtcagctcc gaaacggatg caatgatgcg ttcggtgagc
     4861 gcgaagccgt aggcaaaatc ggcaagatca gtgggagtgg ccatcatcac ggcgtaaggg
     4921 cgagcgttgt attgcatggc cacaggcact tcggcggcca ccgactcgct caccgcctgg
     4981 ctgaactcgc cttcctggcg tcgcgccgtg acagtctcca gcgggtcacg agctcgcacc
     5041 ccttgcggct tcggtgacac cacggaaccc ggttttgcca tgccagtcgt acccgacctc
     5101 agcctgatat atcagccgga tattgtacgc ttgttgcgaa ctattgcgct gcagcgagga
     5161 ttcgcgcatg gtgctcgagg tgatcgccaa cgaacgtgga gatgaagtag tagttgtggt
     5221 cgtagccggc atgccgtcgc aggcgcagcg gctggtgcga ggactggcag gccgcctcga
     5281 attgctccgg cagcagttgt ccggcggtca gaaatttatc ggccagcccc tgatccacga
     5341 ggatctgcgg cagcagcgcc ctgcccgccg agcgcagcag ctccaccgca tcgtagggcg
     5401 cccacgtgga cctgtcctct ccgaggtagc gcgcaaacgc cttgagtccc cacggacagc
     5461 ggctgggtga gcagatcggc gcgaaggcgg agacgctgcg gaacagatcc ggattcttca
     5521 acgcgatgat gagtgcgccg tggccaccca tcgagtgccc gaagatgccc agctgaccgg
     5581 ggctggccgg gaacgatgcg aggaccagcc cgcgcaattc gtcgcgcaca tagtcgtaca
     5641 tacgaaagta ttgcgaccac ggggcctgcg tcgcgttcag atagaaaccg gcgccactgc
     5701 caaattccca gtcatccgcc tcacccgcga tgccagtgtt gcgcggactg gtatcgcagc
     5761 cgatgagcat cagtccaagc tcggcggcca gccgctgcgc cccacccttg gccgcgaagc
     5821 tctcctcgtt gccggtcagg cccgccagat aaaacagcgt cggcaccgcg ccgcgctcgg
     5881 cttgcggcgg ccggtaaacc gaaaagcgca tcggcagtcc gatggtgctg gaggtgtgac
     5941 ggtaaaaacc ctgcacgccg tcgtagcagc ggtgctcgct gatcgtctca attgtcatgg
     6001 cagcggattt tagctgcgat aatgagcgca tgaacatcga actcagtgcc cacgaagcac
     6061 gcatcctcgg ctgtctggtc gagaagcaga tcaccacgcc cgagcaatat ccgctgtcgc
     6121 tgaatgcgat caccaatgcc tgcaatcaga aaagtaaccg cgatccggta ctggccttga
     6181 gcgagcagga ggtccagagc gtcatcgata cgctgtctcg caagcatctg gtgctcgagc
     6241 gcgccggctt cggcagccgc gttccgaagt accagcaacg cttctgcaac accgagttca
     6301 gcatgctgca gttcacgccg caggagcgcg cgatcgtctg cgagctgctg ctgcgggggc
     6361 cgcagacacc gggcgagctg cgcagtcgcg cggcgcgcat ggccgagttc accgacgcgt
     6421 cgcaggtcga ggcagcactg gaggcgctgc gcacgcgcga aagcggaccg atggtacaga
     6481 ggctgctgcg cgaggcgggc cgccgcgagt cccgctacgc gcacctgttc agtggagcga
     6541 tcatcgagac agctaccgca gagccgcccg ccgttcacgt cgcgtccgcc tcctcagcgc
     6601 cgagcacgag tctgccttcg tccgcgctcg agcgtctgat cgcgctggag gagcaggtgc
     6661 agcagctgcg cgaggaagtc gcgcagctga aactcaaagc ttagtcagcg cggctgagtg
     6721 gccgcgctgc tcaccggcgg catcgcgcgc gcgtcggcgg tggcgggcat cgcggcgatg
     6781 tcacgattgg actccagcag cagcttgccg ctcagattga cgccatcgaa cgcgctgacc
     6841 tcgagcaggg cctcgatcgg atgcgagacg tcggcctgat gggcgaactc ctgcagtcgc
     6901 tgattgctgg tgaaggtctc cgcggtctgg ctgacccctt catcgcgcgt gccggaaatc
     6961 acgaggacga cgttaccgcc gggcccgcga aatttcgcga acactccgta gtcgtgatac
     7021 gcctgctcct tgccgctcgg ctgcggcgga tccatcacct ggctgccgac ttcgctcgca
     7081 taggagtgct tggtcttctt gtcgatgacc tcatcgtagg actcgccgac ggtgaagcgc
     7141 gattccggcg tgaacaccag gtcctgcagc atccccatgc cgctcagata gccgatataa
     7201 atgatgtcat cggccttcag gctgctcggt tccacgtccg acatcttgct gatcgtgata
     7261 cgccggttct gcggcgcgag caccgccatc acatcgcgca gcgcaaacgc ggatgacacc
     7321 ggcagatagc gcaagccgac atcgatgtag cgatcggcca cttccgggtg ctcggcgacg
     7381 aagtgatcca gatcgccctt tgaattgacc gtgtactcgc ggatcaggcg cttgacgccc
     7441 atcgaatcgg tttcaccaat caggtagtag tcgccgacca cgatcatgat cgggcgatcg
     7501 tccttcagca gtgatgacca gatcggattg gcacgcacct gctcgacctc gctgcgtggc
     7561 gcgccccagg ccaggatcgc cccgacgatc agcgccagca cgccggccgc ggccacgccg
     7621 taggccatcc acaccctgcg atcggtgcga cccgacgcct ccggcgcgac cggttcgatc
     7681 tcggcggccg ctttggcgct aatcacccgt aggcggtact cccctttcgg aatctgcagc
     7741 gccggccgcc cctcaccggc gcaagcccgg tagtagtcag ccagcgcgcg gcgcagcttg
     7801 tgcacataga cgcgaaccag cgcgtcctgc gacacatcga agtcagcccc cttgccgaag
     7861 acatcgatcg cgatgctgat ctccttgggc gcatgacccg ccagcgtgct gtcaaccaga
     7921 tagtcaaaca gacgacgtag ccgcgcctcc cccagcaccc cgctggagcg caggtgctcg
     7981 gcttcgacac gaatgagcga actgtcatcc atttgagggc gattttaacg tttagtaacg
     8041 tgtgcttaaa cagtaattta cgtgacctgc gccgagcagg cgttatggtg gatgcgcgtt
     8101 tttctgcgca cccgcccctc aaggaccaac cccatgactg ctaacagttt tgcccgcatc
     8161 ggcgcacgcg tgctgtgtgc cgcgggcgtg ctggcgacca accccgcgat cccggcaggt
     8221 ccggatgcca gggcgattcc ggctgcggga ctcgccatca gccagctcga gctcatgcgt
     8281 gcctcggttc agatccccgc cgatggcatc tggggtgcgg ccggtgccga caaactctct
     8341 gacgatgagt ggctgctggc cgagcaggac tccaccaatc tggtggccgc ggccagcctg
     8401 cttgggatgc ccggcacggg caagaaggat cggaactgga ttgcgagtgc ggactggcag
     8461 gggtgggtgc gcgagttccg gcgcacggcg ctcgagctgc acgcggccgc caaagctcac
     8521 gatcttccca agctgtccgc ggcaggtgac cacctcacca aggtgtgcga ggactgccac
     8581 gccaaatatc gccccgcgac gccctcggac ggggtgagcc gctatccgtt ctatccgaag
     8641 cgcgagctgc cttcgcagta aacgccgcgc gcatggcagc ccttcgaacg tgctccccgc
     8701 agccaggcat cccgcggggt ctgatcagtt tcgcgcttcg cgcgttgctg gggctcgcga
     8761 cagcagcccg ggcggatgac acttcgagtg ccgacgatca gtcgagcagc caggccctcg
     8821 aagaaatcag cgtcacggca cagcgcctgc agctgctcgg aagcgccgag tcagccagcg
     8881 aagggatcgt cgcggaccag gagctgcagc tcacgcccgc ctatcgaccg gcgcaactgc
     8941 tggagacggt ccctggcctc acggtcaccg cgcacagcgg cgagggcaaa gccaatcagt
     9001 atctgctgcg cggctacaac ctcgatcacg gcaccgatct ggcgaccttt gtcgacgcga
     9061 tgccggtcaa tcagccgacg cacgcccatg gccagggata caccgacttg agtttcatga
     9121 ttccggaact ggc
//
LOCUS       DATMRM010000179         4452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2271247, whole genome shotgun sequence.
ACCESSION   DATMRM010000179 DATMRM010000000
VERSION     DATMRM010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2271247"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VNZ06_05240"
     CDS             complement(<1..823)
                     /locus_tag="VNZ06_05240"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWW20189.1"
                     /translation="MPYVENRIVHDADSHLMELPDSLDDFLDGRLRAQYDALPKLRKQ
                     PRNADYVLRARAMQQDAEFRKGADENILLRKNYEALGAFRREDRPRALDDLGFASQLI
                     FTTWCLGNFDLDESRDIELCYGVAQAHNRMMSSFCSVDRRFLATGYIPLVDFARAITC
                     AQEAIKLGCKALLLPSRCPRSHSPSHIAFEPLWALAQEAGLPIVFHVGGEEKLPPGYF
                     ENGLPRVKDFHGGEENFTSVSFMPIPHSVQLTLAALIIDGVLDRYPRLKFGAIELG"
     gene            complement(851..1882)
                     /locus_tag="VNZ06_05245"
     CDS             complement(851..1882)
                     /locus_tag="VNZ06_05245"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWW20190.1"
                     /translation="MTTISRRDFLVTVPMLGVAAAASAQTPTPAAPAAATPPLPQYNF
                     PPPTLPMTPLTDGLKFPEGPVAMDDGSVLFVQIESMQISRLTPDGKVSLVKQLEGGPN
                     GLAIGPDKALYIANDGGRFGFVKRGGYNFPGAAPPEHTGGSVQRLDLKTGELTTLYET
                     CDGKRLVAPDDLVFDRHGGMWISDFGKKADGGVYYATHDGKKIVCAKPMNSPNGIGLS
                     PDGKQLHISERTQLWTFDIEKPGVLSATPKSYPNGEHAKLRERSYADSLKVQADGKVC
                     VCTLIAGGISIIDQAGATEFMQFNDPMVTNLTFGGKDMRDCWLTLSATGKMAKVRWPY
                     AGLKPAYHA"
     gene            complement(2034..2555)
                     /locus_tag="VNZ06_05250"
     CDS             complement(2034..2555)
                     /locus_tag="VNZ06_05250"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HWW20191.1"
                     /translation="MAISLYDATIPSCLQLLAAVSNLLDKAQAFCAEKGLDPNSLLQA
                     RLADDMQPFVYQVKSTRVHSVGAIEGVRRGSFSPDTSTPPQTFPDLKIQVAETIEALK
                     AVEPSEIESFIGRDVRFVFGERQVPYTAENFLLSFSQPNFYFHATTTYDILRWKGVPI
                     GKRDFIGRPRFKQ"
     gene            2666..3253
                     /locus_tag="VNZ06_05255"
     CDS             2666..3253
                     /locus_tag="VNZ06_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWW20192.1"
                     /translation="MPALHSVEISSEVLERARAGDTDAHAYLYRTLSKPVYTLLRRFV
                     VRPAIAEELLQDVFVEILRNLNHYSGIGSFPGWVRSIAVNKALMYLRSPWHRSLSWLG
                     ADGIQSIHEESVAAIHDSFDSDLERALAALPAVSRAVVWLHDVEEMTHAEIARLFGRT
                     ASFSKSQLARAHRELRDRLETSSGGLACMPISRNC"
     gene            3226..3885
                     /locus_tag="VNZ06_05260"
     CDS             3226..3885
                     /locus_tag="VNZ06_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20193.1"
                     /translation="MHADFQELLNLRAGEPVAADVAQHVSKCPKCGLELGRLQRVAHE
                     LQQLPQFEPPQRVWLTVRERLLRSAPLHSHGGWIYLSTAAAAIVAIVTGVLWTKTIDR
                     DNTARHVAVVAGSALPVNDHDSIAPLVVRSQELEALLRQMPQRPTVERAATSATIDEI
                     QSTIQMLDLQLSNSSRLDHEEAKRLWNTRVQLLDSLVYVRYAEVARDARPINTLDTGA
                     I"
     gene            4044..>4452
                     /locus_tag="VNZ06_05265"
     CDS             4044..>4452
                     /locus_tag="VNZ06_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWW20194.1"
                     /translation="MDSNKTEQAHLERELQRAQEELNRAAREVAELSTRMREEAWSQI
                     ETNMDFGLQRSQLGINIGVVHGRGDESTDGVRILSVSPGGPAEKAGLKANDVIVSVGG
                     TQLRGDRKTSAQEQLLAQMRRTQPGTPVAVEYRR"
BASE COUNT          852 a         1376 c         1358 g          866 t
ORIGIN      
        1 ctccgagttc gatcgcaccg aatttgagtc tcgggtaacg atccagcacg ccgtcgatga
       61 tgagcgcggc cagcgtcaac tgcaccgagt gcgggatcgg catgaaactc accgacgtga
      121 aattctcttc gccgccgtgg aaatccttga cacgcggcag tccgttctcg aagtagcccg
      181 gaggaagttt ctcctcgcca ccgacgtgaa acacgatcgg caggcctgcc tcctgcgcca
      241 gcgcccacag tggctcgaac gcaatgtgac tcggtgaatg gctgcgcgga cagcgtgacg
      301 gaagcagcag cgccttgcag ccgagcttga tggcctcctg tgcacaggtg atggcgcgcg
      361 cgaaatcaac cagcggtata taaccggtcg ccaggaaacg ccgatcgacc gaacagaacg
      421 aactcatcat ccggttgtgc gcctgcgcca cgccatagca cagctcgatg tcacgggatt
      481 catccagatc aaaattgccc agacaccagg tggtgaagat cagctgactg gcgaagccca
      541 gatcgtccag cgcccgtggc cgatcctcgc gacgaaacgc accgagcgct tcgtagttct
      601 tgcgcagcag aatattctcg tctgcgccct tgcgaaattc agcatcctgt tgcatggcgc
      661 gtgcccgcag cacgtaatcg gcattgcgcg gctgcttcct gagtttgggc aatgcgtcgt
      721 attgcgcgcg caatctgcca tcgaggaaat cgtccagcga gtcaggcagc tccattaagt
      781 gagagtcggc atcgtgcacg atgcggtttt ctacgtaagg cataacgagc tccgtgttcc
      841 ggtaacggcg ctatgcgtga tacgccggct tcagtcctgc atacggccag cgcactttcg
      901 ccatcttccc agtggccgaa agcgtcagcc agcaatcgcg catgtccttg ccgccaaagg
      961 tgaggttggt gaccatggga tcattgaatt gcatgaattc ggtcgcgccg gcctgatcga
     1021 tgatgctgat gccacccgcg atcaacgtgc atacacatac cttaccgtcg gcctgcactt
     1081 tcaggctgtc agcataggag cgttcgcgca gcttggcgtg ctcgccattc gggtaggact
     1141 tcggcgtcgc ggacaggaca ccgggcttct cgatatcgaa cgtccagagt tgcgtgcgct
     1201 cgctgatatg tagttgcttg ccatcgggcg acagtccgat gccgttgggg gagttcatcg
     1261 gcttggcgca cacgatcttt ttgccgtcgt gcgtcgcgta gtacacgccg ccgtcggcct
     1321 tcttgccgaa gtcgctgatc cacataccac cgtggcgatc gaataccaga tcatccggcg
     1381 cgaccaatcg tttgccatcg caggtctcat acagcgtcgt gagttcaccg gttttcagat
     1441 ccagccgctg gaccgaccca ccggtatgtt ccggcggagc cgctcccgga aagttatagc
     1501 cgccacgctt gacgaaaccg aagcgcccgc catcattggc gatgtacaac gccttatccg
     1561 gtccgatcgc gagcccgttg ggccctccct ccagttgttt caccaggctg actttgccat
     1621 caggtgtcag ccggctgatc tgcatcgatt cgatctgaac gaacagtacc gaaccgtcgt
     1681 ccatggccac cggaccttcc gggaatttca gtccgtcggt gagcggggtc attggcaagg
     1741 tcggcggcgg aaagttgtac tgcggcagcg gcggggtcgc ggccgccggg gctgcaggcg
     1801 tcggagtctg ggcactcgcg gcggcagcga cgcccagcat cggtaccgtc accagaaagt
     1861 ctcgtcggct gatcgttgtc atgacgtccc tcgtctatgg ccagtggcgc ctcgggcggc
     1921 ctactgccgc agcaacgaca gaccgtcccc ccgtttacgt tgtggtgccc gccgcctagg
     1981 gcgggctgac cctattgtac tatttattca ctaagacggg gcgcagccgg actttactgc
     2041 ttgaatcgtg gcctgccgat gaagtcccgc ttgccgatcg gcaccccttt ccatcgcagg
     2101 atgtcgtagg tcgttgtggc gtgaaagtaa aagttcggct gcgagaacga cagcagaaaa
     2161 ttctccgccg tgtagggtac ctgtcgttca ccgaacacga agcgcacgtc gcgcccaatg
     2221 aagctctcga tctctgacgg ctcgacggcc ttgagcgctt cgatggtctc ggccacctgt
     2281 attttcaggt ccggaaaggt ctgcggcggt gtcgaggtat caggcgaaaa agagcctcgg
     2341 cgtactccct cgattgcgcc cacggaatga acccgggtgg atttcacctg gtagacgaac
     2401 ggctgcatgt cgtccgccag gcgcgcctgt agcaggctgt tgggatccag acccttctcg
     2461 gcacagaacg cctgtgcctt atcgagcaga ttggacacgg ccgcgagcag ctgcaggcag
     2521 ctcggaatgg tggcgtcgta gagtgagatt gccatgtggg gacccccggt agagcctgtg
     2581 taggttggta agggagtcta cccggacccc gtctaaagac gactgcctgc aaccttttgc
     2641 gggggtgctg catctatagc gaggtatgcc ggcactgcac agcgtagaaa tatcgtcgga
     2701 agtcctggaa agggcccgag ctggtgacac cgatgcgcac gcctacctct accgaaccct
     2761 ttcaaagccg gtctacaccc tgctgcggcg ctttgtggtt cgtccggcga tcgccgagga
     2821 gctgctgcag gacgtgttcg tggaaatcct gcgtaatctc aatcattaca gcggcatagg
     2881 gtcattcccg ggttgggtcc gtagcattgc cgtcaacaag gccctgatgt atttgcgatc
     2941 gccttggcac cgcagtctga gctggttggg cgccgatgga attcagtcca tccacgagga
     3001 gagcgtcgcg gccatccatg attcgttcga ctccgacctc gagcgggctc tggcggcatt
     3061 gccggcagtc tctcgggccg ttgtctggct gcacgatgtc gaggaaatga cacacgcgga
     3121 aatcgcgcga ctgttcgggc gcaccgccag cttctccaaa tcccagctcg cgcgcgcgca
     3181 tagggaattg cgcgaccgat tggaaacttc ttccggagga ctcgcatgca tgccgatttc
     3241 caggaactgc tgaacctgcg cgccggcgag ccggtcgctg ccgacgtcgc gcagcacgtt
     3301 tcgaaatgtc ccaagtgcgg actcgaactg ggtcggctgc agcgcgtcgc tcacgagctg
     3361 cagcagttgc cgcagttcga acccccgcag cgggtgtggc tgactgtacg ggagcggctg
     3421 ctgcgcagcg ccccgcttca cagtcacgga ggctggatct atctgtcgac cgcagcggca
     3481 gcgatcgtcg cgatcgtaac cggcgtactg tggaccaaaa ctatcgatcg cgacaacacc
     3541 gcgcgtcacg tcgcggtcgt ggccggatct gctcttcctg tcaacgatca tgactcgatt
     3601 gcgccgctcg tggttcgctc gcaggagctg gaggcgttgc tgcgacagat gccgcagcgt
     3661 ccgaccgtag agcgcgccgc cacatcggca acgatcgatg agattcaatc cactatccag
     3721 atgctcgacc tgcagctgtc gaacagttcg cggctcgatc acgaagaagc caagcgcttg
     3781 tggaacacgc gcgtccagtt gctggactcg ctggtctacg tgcgctacgc agaggttgca
     3841 cgcgacgcac gccccatcaa cacactcgat accggagcta tctaatgaac gtttaccacg
     3901 cccgcgcgct cgcgctgctt gcggtggtcg cgggtgcagc ctatgcagcc gatgcgcccc
     3961 cgacacctgt ttcacccgcc tcaccggcgt caccagctgc gccagcctct gcgacgactc
     4021 cacccgcgcc tccaagccca tcgatggact cgaacaagac agagcaggcg cacctggagc
     4081 gagaactgca gagagcgcag gaagaactca atcgcgccgc gcgcgaggtc gcggagctga
     4141 gcaccagaat gcgtgaggag gcctggtctc agattgaaac caacatggac ttcggtttgc
     4201 agcgcagcca gcttggcatc aacatcggcg tggttcatgg gcgaggggac gaatcgactg
     4261 acggcgtgcg catcctcagt gtcagcccag gaggtccggc ggagaaggct ggcctgaagg
     4321 ccaacgacgt catcgtctcg gtgggcggca cgcagctgcg cggcgatcgg aagacgtcag
     4381 cgcaggagca gctgctggcg cagatgcgcc gcacgcaacc cgggacacct gttgcggtgg
     4441 agtatcgccg cg
//
LOCUS       DATMRM010000180        13501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_429812, whole genome shotgun sequence.
ACCESSION   DATMRM010000180 DATMRM010000000
VERSION     DATMRM010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13501
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429812"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VNZ06_05270"
     CDS             complement(<1..319)
                     /locus_tag="VNZ06_05270"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20195.1"
                     /translation="MRVRDCAIALLAGAALTCAFAPLNAWPLAFLCPAILMWVWEGST
                     WRHAALTGFCFNAATFGCGTSWMYIGVHDFSGAPAWIAIGLVIGLTGIMAAYHALLGY
                     LSVR"
     gene            complement(316..1197)
                     /locus_tag="VNZ06_05275"
     CDS             complement(316..1197)
                     /locus_tag="VNZ06_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HWW20196.1"
                     /translation="MARDKTTPPVNTATSGRWRMRITRSLAAAGLKDRGQLIEVLSDA
                     QQRGVLDADAFAMLEGALAVGDLQVRDIMIPRVQMVCMRRDDPISKILPAVVDSGHSR
                     FPVVDGDRDDVVGILLAKDLLRLFASAPTARFDIREYLRPVVFVPESKRLDVLLKEFR
                     NNHNHMAIVVDEYSGVAGLVTIEDVIEQIVGDIDDEFDVEDDQNIRREADRQFSVRGS
                     TRIDEFNQYFRAELPEGEFDTIAGLVMKQFGRMPRRGESASLDEFEFRVTRADRRRID
                     TLRVTIPAKFEPVAPAE"
     gene            complement(1184..1669)
                     /gene="ybeY"
                     /locus_tag="VNZ06_05280"
     CDS             complement(1184..1669)
                     /gene="ybeY"
                     /locus_tag="VNZ06_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWW20197.1"
                     /translation="MTPGACISVARRVRRGWTPPARDFRLWADTVLGAGAARHEISVL
                     LVGERRSRRLNARYRGRDCSTNVLAFPAPPAAARSSGLLGDLVICPAVLRAEAHEQDK
                     DVRSHWAHMVVHGLLHLLGYDHERQSDAQRMERREVRVLRQLGILNPYVVRSKRSHGQ
                     G"
     gene            complement(1666..2673)
                     /locus_tag="VNZ06_05285"
     CDS             complement(1666..2673)
                     /locus_tag="VNZ06_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWW20198.1"
                     /translation="MNPPHAASREFALEPVDNSRLASLCGPLDANLRLVESRLGVQIR
                     RRGHSFRIQGARAASTENVLRDLFAQTKHHEVTPEQVHLCLVEHSNGQDPGAGADVVI
                     AHAPRGHEHEGETGTHGRRGTIKARGTHQREYLANIHGHDLTFGVGPAGTGKTYLAVA
                     CAVEALAADRVRRIVLVRPAVEAGERLGFLPGDMAQKVDPYLRPMYDALYEMMGYDRV
                     ARFIERNAIEVAPLAFMRGRSLNESFIILDEAQNTTIEQMKMFLTRIGFGSRAVVTGD
                     LTQTDLPTGRMSGLHHAQLILRGVQGVAFTFFDARDVVRHELVQRIVQAYESHEVVRE
                     P"
     gene            complement(2697..4019)
                     /gene="miaB"
                     /locus_tag="VNZ06_05290"
     CDS             complement(2697..4019)
                     /gene="miaB"
                     /locus_tag="VNZ06_05290"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HWW20199.1"
                     /translation="MPGSFYIKTFGCQMNEYDSARMADVLREGVGLSPTDAPERAELL
                     LMNTCSVREKAQEKVFSLLGEWRALKSVNPGVIIGVGGCVASQEGAEITRRAPFVDLV
                     FGPQTLHRLPEMIGEFKARGRSIVDVSFPEIEKFDRLPQPRAQGARAYVSVMEGCSKY
                     CSFCVVPYTRGDEISRPFESVLDEVHALVRQGVSEIVLLGQNVNAYAGAMSDGAVVDL
                     ATLIHHVAAIEGVARIRFTTSHPLEFSDSLIEAYANVPKLANHLHLPVQSGSDRILAL
                     MKRGYTSLEYKHRIRKLRAVRPGISVSTDIIVGFPGETERDFEATLRLVADVQFDQSF
                     CFIYSRRPGTPAASLPDEVELSLKRERLARLQALLDAQSLAISDAMVGGVQRVLVERS
                     AKKGHSELAGRTENNRWVNFPGPASLLQRFVDVLVTEARPHSLRGRLA"
     gene            complement(4118..4240)
                     /locus_tag="VNZ06_05295"
     CDS             complement(4118..4240)
                     /locus_tag="VNZ06_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20200.1"
                     /translation="MTPELAGIARDELGAAAGSVMVWRSVILPALDFLIVGFGV"
     gene            complement(4247..4741)
                     /gene="ssb"
                     /locus_tag="VNZ06_05300"
     CDS             complement(4247..4741)
                     /gene="ssb"
                     /locus_tag="VNZ06_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWW20201.1"
                     /translation="MARGVNKVILIGHLGADPETRAMPSGMQVANLRLATTESWKDKS
                     SGEQQERTEWHSVALFGRLGEIAAEYLRKGSQVYIEGRLRTRKWQDKEGRDRYTTEIV
                     GSEMQMLGGRGGAGGGGGGGGGGYSEAPSRGEPAEAAMSGGAGSSRGAGSSAPEEFDD
                     DIPF"
     gene            complement(4970..8395)
                     /locus_tag="VNZ06_05305"
     CDS             complement(4970..8395)
                     /locus_tag="VNZ06_05305"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWW20202.1"
                     /translation="MINRSLGAWLICLLPVCVTSAVAADDPRLMLRFPTLSRTQIAFN
                     YGGDLWVVGREGGEAHRVTSSMGSQTTPYFSPDGRWIAFSGDYDGNRDVYLVSSAGSV
                     PRRITWHPSPDTVRGWTPDGKRVLFSSSRGFETTQDQLYTTSTDGGFPTPLPLPSAES
                     ASYSPDGSHVAYVPHFVFEPNWKRYQGGQTTPIWIADLKDSSVVVVPRDNSVDRNPMW
                     VGEKIYFLSDRNGPVSLFAYDTQSRQVTEVLHSDGLDFKSASAGPDAIVIEQFGAIKL
                     FDLKTGQAHTVDIRVQGDLPAVRPRYVHVKADQVQHFDFSPTGARAVFEAWGEIFSVP
                     TDKGDIRNLTHSPAVAERDPSWSPDGKSIAYFSDASGEYALQILDQSGQKAARSISLG
                     NPPSFFYSPRWSPDNKKIAYADKRLGLWYMDVASGAPKRIDAGMWGGFGSDDFQIAWS
                     PDSHWLTYVKALRNGQHAVFAYSLDQGEALQLTDGLSDAQFPVFDRNGKYLYFTASTD
                     VALTVQGLDMTSDAHPVTRSVYVIVLSREEKSPLAPQSDEDKGVSQKDADKAKSDDKD
                     KDKDSSGSDEKGGKHARDSKHDQDSSEDKDKDKDKDNKDKEGKTPHVRIDIEGISQRI
                     LALPIPARNYADMRAGASGILFIAEAPRVIEADDFPNPAEVLHKFDLSKRKIDKFIDE
                     INDFRVSDDGKKILYRKGEQFTVVSAEDAEASGKPKPGFGPLQLNDWQVYIEPQKMWR
                     QIYDESFRIERDFFYDPHYHGVDLAKLKKKYEPYVAGIASRDELSYLLEEVYSELTVG
                     HLYVRGGDRLEVRHVKVGLLGADYSLDAGRYSFEHVYDGENWNPKLQAPLTQPGVNVK
                     AGEYLLAVNSRELHASDNLYSFFEATAGKQVVLRVGPKADGSGAREVTVVPVDDEHAL
                     RQRAWIEDNRRRVDQETGGRVAYVYVPDTGNNGFTSFNRYFFAQIGKEAVIVDERFNK
                     GGQLADYIVDYLNRKLLNKVVTREGLDWDSPSQAIYGPKVMLINEMAGSGGDALPWYF
                     RKMAIGALIGKRTWGGLVGIGGYPQLIDGGTITAPRAAIYGLKGEWEVENHGVAPDYE
                     VELDPQAYRAGHDSQLDKAIQVVMQQLAEHPPAQIKRPPFPNYHPHDELGSSH"
     gene            8535..11411
                     /gene="uvrA"
                     /locus_tag="VNZ06_05310"
     CDS             8535..11411
                     /gene="uvrA"
                     /locus_tag="VNZ06_05310"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWW20203.1"
                     /translation="MQTIRIRGARTHNLRSIDLDLPRERLTVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSMMDKPDVDSIEGLSPAIAIEQKSGSHNPRSTVGTVT
                     EIYDYLRLLYARAGLPRCPRHGIDLAAQSVSQMVDQVLQAPQGELALLLAPLIDERKG
                     EHAQVIAELAGQGFVRVRIDGTVYEIDAVPKLDPKRKHSIEAVVDRFRVRPDTSQRLA
                     ESFETAIRISQGVVRVVFPDAPALSAMLFSSRHACPECGFSVPPLEPRMFSFNNPAGA
                     CPTCDGLGFQEFFDPERVVVHPHLSLAGGAVRGWDRRNAHYFQMLQGLARHFQFDVET
                     PWDELEPRVRQVLLSGSGDEEIEFRYQEARTPVKRRHTFEGIVPNLARRFRETDSQAV
                     REELRKYLGTRDCIDCNGARLNEAARSVLIEGRSLPEITRMTVGNARGYFDALSLTGW
                     RAEVAARIVREVGERLRFLVDVGLDYLSLDRSAASLSGGEAQRIRLASQVGSGLTGVM
                     YILDEPSIGLHQRDNARLLGTLKRLRDLGNTVIVVEHDADAIRAADHVVDLGPGAGAH
                     GGLVVAQGTPAQIESDPASLTGAYLSGRQSVALPERRRSPQPNQWLSIRGARSNNLKN
                     ITVDLPIGLMTCVTGVSGSGKSTLVEDTLFRHAAVRLNGANPDGAGVCDEIVGLERFE
                     RVIEIDQNPIGRTPRSNPATYTGVFTIIRELFASVPEARARGYDAGRFSFNVKGGRCE
                     ACQGDGVIQVEMHFLPDVYVPCDVCRGKRYNRETLEIQYRGRNVHQVLDTTIEEALQL
                     FENVPAVAGKLRTLMDVGLTYVRLGQNATTLSGGEAQRVKLARELAKRGAGRTLYILD
                     EPTTGLHFHDIAQLLRVLQQLRDAGNTLVIIEHNLDVIKTADYVIDLGPEGGERGGRV
                     VASGTPEAICDQPESVTAQYLAPLLQRVSEPAAIRTRSAGAPRR"
     gene            complement(11359..11898)
                     /locus_tag="VNZ06_05315"
     CDS             complement(11359..11898)
                     /locus_tag="VNZ06_05315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWW20204.1"
                     /translation="MNQVSLRDGRHNRDDRRWIESVYHDYLTDLAPTATGIFPALGEV
                     GHRVPDQLMHWYSDRSAHVMTVVYGLERAGFAMVEQRLQAVASGGAAGTSPTIEYSMK
                     EFFIARQWRRRGIGAQAVRLLLDRFTGRWLITEHMRNATAVSFWRRVVCAYTNGRYQE
                     RIVNGEVHQRFESGSRRVR"
     gene            11897..12262
                     /locus_tag="VNZ06_05320"
     CDS             11897..12262
                     /locus_tag="VNZ06_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20205.1"
                     /translation="MNSLRKQESPADRILPACSGFPASLELVGFRKTRSYFGAEPPED
                     GVVAELLLVPSDGAIAPLLLPSVAGAMVADGVVAAGAEAGGGVLSVLLQAESTSAAAR
                     ALRASFVFIDESPELYERS"
     gene            complement(12616..12972)
                     /gene="rplQ"
                     /locus_tag="VNZ06_05325"
                     /pseudo
     CDS             complement(12616..12972)
                     /gene="rplQ"
                     /locus_tag="VNZ06_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(13094..>13501)
                     /locus_tag="VNZ06_05330"
     CDS             complement(13094..>13501)
                     /locus_tag="VNZ06_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HWW20206.1"
                     /translation="IDIETNGTIEPEEAIRRAGAILKDQLSVFVDLQGEDEAVAKTTE
                     MQFDPILLRPVDELELTVRSANCLKAENIHYIGDLVQRTEVELLRTPNLGKKSLTEIK
                     EVLQSHGLMLGMRLEGWPPAGLRHDEESGSGLI"
BASE COUNT         2443 a         4365 c         4124 g         2569 t
ORIGIN      
        1 ctcgcaccga gaggtacccc aacagtgcgt gataggcggc catgatcccg gtcaggccga
       61 tcaccagccc gatcgcgatc cacgcgggtg cacccgagaa gtcgtgtacc ccgatataca
      121 tccaggaagt gccgcaaccg aaggtcgctg cgttgaagca gaagccggtc agggcggcgt
      181 gtcgccaggt actgccctcc cacacccaca tgaggatcgc cggacacagg aacgccagcg
      241 gccacgcatt tagcggtgcg aacgcgcagg tcagcgctgc gccggcaagg agcgcgatgg
      301 cgcaatcgcg cactctcact cggctggcgc cactggctcg aatttggcgg gaatcgtaac
      361 gcgcagtgta tcgatgcggc gtcggtcggc gcgggtcacg cgaaactcaa actcatccag
      421 cgatgcgctc tcgccccggc gcggcatgcg cccgaactgc ttcatgacca gcccggcgat
      481 cgtgtcgaat tcgccttccg gcagttcggc gcgaaagtat tgattgaact cgtcgatgcg
      541 ggtgctgcca cggacgctga actgtcgatc ggcttcgcgc cgaatgttct gatcgtcctc
      601 gacgtcgaac tcatcgtcga tgtcaccgac gatctgctcg atcacatcct cgatcgtgac
      661 caggccagcg acgccactgt actcgtcgac cacaatcgcc atgtgattgt gattgttgcg
      721 gaactccttg agcagtacat ccaggcgctt ggattccggg acgaatacca cgggacgcag
      781 atactcgcgt atgtcgaagc gcgcggttgg cgcactggca aacaggcgca gcaggtcttt
      841 cgccagcaga atgccgacca cgtcatcgcg atcaccgtcc accactggaa agcgcgagtg
      901 accggagtcc acgaccgccg gcagaatttt cgagatggga tcgtcgcgtc gcatgcacac
      961 catctgcacg cgcgggatca tgatatcgcg cacctgcaga tcgcccacgg caagggctcc
     1021 ctccagcatc gcgaacgcat cggcatcgag cacgccgcgc tgctgcgcgt ccgacagcac
     1081 ttcgatcagc tgcccgcgat ccttcaggcc cgcggccgca agactgcggg taatgcgcat
     1141 gcgccagcgt ccactggtag cggtattgac cgggggcgtg gttttatccc tggccatggc
     1201 tgcgcttgct tctgacaacg tagggattga ggattcccag ctggcgcagc acgcgtactt
     1261 cgcgccgttc catacgctgg gcatcgctct ggcgctcatg gtcgtaaccc agcaggtgca
     1321 acaggccgtg caccaccatg tgcgcccagt gcgagcgcac atccttgtcc tgctcgtgcg
     1381 cctcggcacg caacaccgcc ggacaaatca ccagatcccc gagcaagccc gaacttcgcg
     1441 ctgccgccgg tggcgccgga aaggcgagca cgttggtcga gcagtcgcgg ccacgatagc
     1501 gcgcgttgag gcggcggctg cgccgctcac cgaccagcag taccgagatc tcgtgtcttg
     1561 cggcaccggc gccgaggacc gtgtcggccc acaaacgaaa atcgcgcgcc ggcggcgtcc
     1621 agccacggcg tacccgacgc gcaacgctga tgcaggcgcc tggcgtcatg gctcgcgcac
     1681 cacttcgtgc gattcgtacg cctgaacgat gcgctgcacc agctcgtgcc gcaccacatc
     1741 gcgcgcatcg aagaacgtga aggccacacc ttgcacgccc cgcaggatca actgcgcgtg
     1801 atgcagtccg gacatgcgcc cggtaggcag atcggtctgc gtcagatcgc cggtgaccac
     1861 ggcgcgcgag ccaaatccga tgcgggtcag gaacatcttc atctgctcga tggtcgtgtt
     1921 ctgcgcctca tccagaatga tgaaggactc gttgagcgag cggccacgca tgaacgccag
     1981 gggcgcgacc tcgatggcgt tgcgttcgat gaaacgcgcc acgcggtcgt aacccatcat
     2041 ctcgtacagc gcgtcataca tggggcgcag gtacgggtcc actttctgcg ccatgtcccc
     2101 tggcagaaac cccaggcgct cgccggcttc gactgccggc cgcaccagca cgatgcggcg
     2161 cacgcgatcg gcagcgagcg cttccaccgc gcacgctacc gccaggtagg ttttgccagt
     2221 accggccggt ccgacaccaa acgtcagatc gtgcccgtga atgttcgcca gatattcacg
     2281 ctgatgagtg ccacgcgcct tgatcgtgcc gcgacgccca tgggtaccgg tctcaccctc
     2341 gtgctcgtga ccgcgtggcg cgtgagcaat cacgacatcg gcgccagcgc ccggatcctg
     2401 gccgttggag tgctccacca gacacaggtg tacctgttcg ggcgtgacct cgtggtgctt
     2461 ggtttgcgcg aacaggtctc gtaacacgtt ctcggtcgaa gccgcccgcg caccctgaat
     2521 gcggaacgaa tggccacggc ggcggatctg cacacccaat cgcgattcca ccaggcgcag
     2581 attggcgtcg agcggaccac acaggctggc gagccgtgag ttgtcgaccg gctcgagggc
     2641 aaattcgcgc gatgcggcgt gcggaggatt cacaggactg tcagctcaag gccacttcag
     2701 gccaaccgtc cgcgcaggga gtgtggccgc gcctcggtga ccaagacatc cacgaagcgc
     2761 tgcagcaacg acgccggccc cggaaaattc acccagcgat tgttctcggt acgtccggcg
     2821 agttcgctgt ggcccttctt ggccgaacgc tccaccagca cgcgctgtac gccgccgacc
     2881 atcgcatcgc tgatggccag cgactgggca tcgagcaagg cctgcaggcg tgcgagtcgt
     2941 tcgcgcttga gcgacagctc aacctcatcg ggcagtgatg cggccggtgt gccgggacga
     3001 cggctgtaga tgaagcagaa cgattgatcg aactgcacgt ccgccaccag cctcaaggtc
     3061 gcttcgaaat cacgctcggt ctcccccgga aagccgacga tgatgtcggt ggagacggaa
     3121 atgcctggtc tcacggcccg cagtttgcga atgcgatgct tgtactcgag cgacgtgtag
     3181 ccgcgtttca tcagcgccag gatgcgatcc gagccgctct gcaccggcaa gtgcagatga
     3241 ttcgccagct tcggcacatt ggcgtacgcc tcgatcagac tgtcgctgaa ctcgagcgga
     3301 tgcgaggtgg taaagcggat gcgcgcgacc ccctcgatgg ccgcgacgtg atgaatcagc
     3361 gtcgccagat caaccacagc gccatccgac atcgcaccgg cgtacgcgtt gacgttctgc
     3421 ccgagcaaca cgatctcgct cacgccctgc cgcaccagcg catgcacttc atcgagcacc
     3481 gattcgaacg gccggctgat ttcatcgccg cgcgtatacg gcacgacgca gaaactgcaa
     3541 tatttgctgc agccttccat cacggatacg taggcgcgtg cgccctgggc gcgtggctgc
     3601 ggcaggcggt cgaacttctc gatctccggg aagctcacgt ccacgatcga gcggccgcgc
     3661 gccttgaact caccaatcat ctcgggaagg cgatgcaggg tctgcggccc aaacaccagg
     3721 tccacgaagg gtgcgcgccg ggtaatctcc gcgccctcct ggctcgcgac gcagccgccg
     3781 acgccgatga tgacccccgg attgacggac ttgagcgcgc gccattcgcc cagcagcgag
     3841 aacactttct cctgcgcctt ctcgcgcacc gagcaggtat tcatcagcag cagttcggcg
     3901 cgctccggag cgtcggtggg ggagagcccc acgccttcac gcaatacgtc cgccatcctg
     3961 gcggagtcgt actcattcat ctggcatccg aaggtcttga tgtaaaagga accgggcatt
     4021 ccgaagggct gctcaaaatg gactgcagag ggtagcggtt ttggcttgcg agtgcccgta
     4081 gcaacgcatc aatctattat atatcaataa gttacagtta cactccgaat ccgacgatca
     4141 ggaaatccag cgccggcaga atgacactgc gccataccat cacactaccc gcagccgcgc
     4201 ccagctcgtc gcgtgcaatg ccggcgagct ccggggtcat ctcacactag aacggaatgt
     4261 cgtcgtcgaa ctcctccggc gcgctgctgc cggcgccgcg gctggagcca gcacccccgc
     4321 tcatcgcagc ttccgccggt tcgccgcgcg agggcgcctc gctgtagcca ccacccccgc
     4381 cgccaccgcc gccgccggca ccgcctcgcc cgccgagcat ctgcatttcg ctgccgacga
     4441 tttcggtcgt gtagcggtcg cggccttcct tgtcctgcca cttgcgcgtg cgcagccggc
     4501 cctcgatgta cacctgtgag ccttttcgca ggtactcagc agcaatctcg ccgaggcgcc
     4561 cgaacagcgc gacgctgtgc cattcagtgc gttcctgttg ctcgcccgag gacttgtcct
     4621 tccaactctc cgtcgtcgcg aggcgcaggt tcgccacctg catgcccgag ggcatggccc
     4681 gagtctcagg atccgcgccc aagtgaccga taagaatgac tttgtttacg ccgcgcgcca
     4741 tgatgatgcc tcacttgaat tcgtcttggc ttaagacttt gtcctgttct aagcaaacct
     4801 gtccccgtac ctaattgctg tattcgtgtg gatacgggca agccattggg cgagcccgga
     4861 tcgagaaaag ctggttcgga ggatgcccag aatagtactc caaatgccgc gcaacgcacg
     4921 gcgcgtggtc agtggcggct cccacggtag cggagccgat caccaccatt cagtgcgacg
     4981 accccagctc atcgtgcgga tgatagttgg gaaagggcgg tcgcttgatc tgcgccgggg
     5041 gatgctccgc gagttgctgc atcacaacct ggatcgcctt gtcgagctgc gaatcgtgcc
     5101 ccgcacgata ggcctgtggg tcgagttcga cctcgtagtc gggcgcaacg ccgtgattct
     5161 cgacttccca ctcgcccttc aatccgtaga tcgcggcgcg cggcgccgtg atcgtgccgc
     5221 cgtcaatcag ttgagggtac ccaccgatgc cgaccaggcc gccccaggtg cgtttgccga
     5281 tcagcgcacc gatggccatc ttgcggaaat accacggcag cgcgtcgcca ccggatcccg
     5341 ccatctcatt gatgagcatg accttggggc catagatggc ttgcgacggt gaatcccagt
     5401 cgagcccttc acgcgtgacg actttattca gcagcttgcg attgaggtag tcaacgatgt
     5461 agtccgccag ctgcccgccc ttgttgaacc gctcatcgac gatcactgcc tccttgccga
     5521 tctgcgcgaa gaaatagcgg ttgaaactgg tgaatccgtt gttgcccgta tccggtacgt
     5581 agacgtaagc gacgcggccg ccggtctcct ggtcgacgcg acgtcggttg tcttcgatcc
     5641 aggcgcgctg ccgcaaagcg tgctcatcgt ccaccggcac cacggtgacc tcgcgcgcgc
     5701 cgctgccgtc ggccttcggt cctaccctca agaccacctg cttgccggcc gtcgcctcga
     5761 agaagctgta gaggttgtcg ctcgcgtgca gctcgcgcga gttgactgcc agcagatact
     5821 cgcccgcctt gacgttgacg cccggttgcg tgagaggcgc ctgcagcttc ggattccagt
     5881 tctcaccgtc atagacgtgc tcgaagctat agcgtccggc gtcgagactg tagtccgcgc
     5941 caagcaatcc gaccttgacg tgcctgactt caaggcgatc gccgccgcgc acgtacaggt
     6001 ggccgacagt cagttcgcta tagacctctt ccagcaggta gctcagctcg tcgcgcgagg
     6061 cgatgccggc cacgtacggc tcatatttct tcttcagctt ggcgagatcc acaccgtgat
     6121 aatgcgggtc gtagaagaaa tcgcgctcga tgcggaacga ctcgtcatag atctggcgcc
     6181 acattttttg cggctcgata tagacctgcc agtcgttgag ctggagcgga ccgaaccccg
     6241 gcttgggctt gccggaagct tcagcatcct ccgccgacac gacagtgaat tgctcgccct
     6301 tgcgatacag gatcttcttg ccgtcgtctg agacccggaa gtcgttgatc tcatcgatga
     6361 acttgtcgat cttgcgtttc gacaggtcga atttgtgcag cacttcggcg gggttcggga
     6421 agtcatcggc ctcgatgacg cgcggcgctt cggcgatgaa caggatcccc gaggcaccgg
     6481 ctctcatatc cgcatagttt cttgccggaa tgggcaacgc caggatgcgt tgactgatcc
     6541 cttcaatgtc gatccggacg tgcggggtct tgccttcctt gtccttgttg tccttatctt
     6601 tgtctttgtc tttgtcttcg ctgctgtcct gatcgtgctt tgaatccctt gcgtgcttgc
     6661 cgcccttttc atccgagccc gaactgtctt tgtctttgtc tttgtcgtcg gacttcgcct
     6721 tgtccgcatc cttctgcgag acacccttgt cttcgtcgct ctgcggcgcg agcggagatt
     6781 tttcctcgcg actgaggacg atcacgtaga cgctgcgcgt caccgggtgt gcatcgctgg
     6841 tcatgtccag cccctgtacc gtcagcgcaa cgtcggtcga tgcggtgaaa taaaggtact
     6901 ttccattgcg atcgaatacc ggaaactgcg cgtcactcag gccgtcggtg agctgcaagg
     6961 cctcgccctg atccagcgaa taggcaaaca cggcgtgctg cccgttgcgc agtgctttga
     7021 cgtacgtcag ccagtggcta tcgggtgacc aggctatctg gaaatcatcg gagccaaatc
     7081 cgccccacat ccccgcatca atgcgcttgg gcgcgccgct cgcgacatcc atgtaccaca
     7141 ggccaaggcg cttgtcggcg tacgcaatct tcttgttgtc cggcgaccat cggggactgt
     7201 agaagaaaga cggcgggttc cccagactga tactgcgcgc cgctttctgg cccgattgat
     7261 cgagtatctg cagcgcgtat tcgccggaag cgtccgaaaa ataggcgatc gatttaccat
     7321 ccggcgacca ggaagggtcg cgctctgcga ccgccgggct gtgcgtgaga ttgcggatat
     7381 cacccttgtc ggtcggcacg gagaagattt caccccacgc ctcgaacacc gcccgtgccc
     7441 cggtcggtga gaaatcaaag tgctgcacct ggtcggcttt cacgtgcaca tagcgcgggc
     7501 gcaccgccgg cagatcgccc tgtacccgga tatcgaccgt atgtgcctga ccggttttca
     7561 gatcaaatag cttgatcgca ccaaactgct cgatgacgat ggcatccggt ccggcggacg
     7621 cgctcttgaa atccagcccg tcgctgtgca ggacttccgt cacctgtctg gattgggtgt
     7681 cgtaggcaaa caggctcacg ggaccgttgc ggtcggacag gaaataaatt ttctcgccca
     7741 cccacatcgg gttcctgtcc accgagttgt cgcgcgggac gaccacgacg ctcgagtcct
     7801 tcagatccgc gatccagatc ggggtcgtct gcccgccctg atagcgtttc cagttcggtt
     7861 caaagacgaa gtgtgggacg taagcgacat gactgccgtc gggcgaatag gaggcgctct
     7921 cggcgctggg cagcgggagc ggtgtcggga atccgccgtc ggtcgaggtc gtgtagagct
     7981 gatcctgcgt tgtttcgaag ccgcgcgacg aggagaacag gacgcgcttg ccatccggcg
     8041 tccaaccgcg aaccgtgtcc ggcgatggat gccaggtgat gcgccgcggc acacttccgg
     8101 cggaggaaac caggtagaca tcgcggttgc cgtcgtagtc accgctgaaa gcgatccatc
     8161 ggccgtcagg ggagaaatac ggcgtggttt gtgaccccat gctggaggtc acacgatgcg
     8221 cctccccgcc ttcgcgcccg acgacccaca gatccccgcc atagttaaac gcgatctgcg
     8281 tccggctgag cgtcggaaat ctgagcatca gcctcggatc gtcagccgcg accgccgatg
     8341 tcacgcagac gggcaacaga caaatcagcc atgcaccgag tgatcgattg atcatggtgg
     8401 acctttattt gcggtgttgt agacggtcgc ggagtcccca ttctagtgcc tttgagccta
     8461 acccgccgca gcgtttatag tggctgtttg gtttctcgac cggcgccttc acgggccttg
     8521 gggagcagcg cagtttgcag accatccgaa tccgaggcgc ccgcacgcac aacctgcgca
     8581 gtatcgacct ggatctgccg cgcgagcgtc tgaccgtggt caccggcctg tctggctccg
     8641 gcaagtcctc actcgcgttc gacacgctgt acgccgaagg ccagcgtcgc tatgtcgaat
     8701 cgctgtcggc ctatgcgcgc cagttcctgt cgatgatgga caagccggac gtggacagca
     8761 tcgaaggcct gtccccagcc atcgcgatcg agcagaagtc cggctcgcac aacccgcgtt
     8821 ccacggtcgg tacggtgacc gagatctatg actacctgcg cctgctgtac gcgcgcgccg
     8881 gactgccgcg ctgtccgcgc cacggcattg atctggcggc gcaaagcgtc agtcagatgg
     8941 tggatcaggt gctccaggcg cctcagggcg aactggcgtt gttgctcgcg ccgctgatcg
     9001 acgaacgcaa aggcgagcat gcgcaggtga tcgccgagct cgccggacag ggattcgttc
     9061 gtgtgcgcat cgatggcacg gtgtacgaga tcgatgccgt gccgaaactc gatcccaagc
     9121 gcaagcacag catcgaagcc gtcgtggatc gcttccgagt ccgtcccgac acctcgcagc
     9181 gtctggcaga gtcgttcgaa accgcgattc gcatcagcca gggcgtggtg cgcgtggtgt
     9241 tcccggacgc ccctgccttg agcgccatgc tgttctcaag ccgccacgcc tgtccggagt
     9301 gcggcttctc ggtgccgccg ctcgagccgc ggatgttctc gttcaacaat ccggctggcg
     9361 cttgcccgac gtgtgacgga ctgggctttc aggagttctt cgatcccgag cgcgtggtgg
     9421 tgcatccgca cctgtcactg gcgggcggcg cggtgcgcgg ctgggaccgt cgcaacgcgc
     9481 actacttcca gatgctgcag gggttggcgc gtcatttcca gttcgacgtg gagaccccct
     9541 gggacgagct cgaaccccgt gtgcggcagg tgctgctgtc tggcagcggc gatgaggaaa
     9601 tcgagttccg ctatcaggag gcgcgtacgc cggtcaaacg gcggcatacc ttcgaaggca
     9661 tcgtgccaaa cctggcacgg cgctttcgcg aaaccgactc acaggcggtg cgcgaggagc
     9721 tgcgcaagta cctgggcacg cgcgattgca tcgattgcaa cggcgcgcgc ctcaacgagg
     9781 cagcccgctc ggtcctgatc gagggccgca gcctgcctga gatcacacga atgacggtcg
     9841 gcaacgcgcg cgggtatttc gacgcgctgt ccctgacagg atggcgggcc gaggtcgccg
     9901 cacgcatcgt gcgcgaggtg ggtgagcgac tgcgcttcct ggtggacgtc ggactggact
     9961 atctgagcct cgaccgtagt gcggcctcgc tctcgggcgg tgaagcacag cgcatccgtc
    10021 tggcaagcca ggtgggctca ggcctcaccg gggtcatgta catccttgat gaaccctcga
    10081 tcgggctgca tcagcgcgac aatgcacggc tgctcggtac gctcaagcgg ctgcgcgacc
    10141 tgggcaacac cgtgatcgtc gtcgagcacg atgccgacgc catccgcgcg gcggatcacg
    10201 tcgtggactt aggtcccggc gcgggcgcgc acggcggcct ggtggtcgcg cagggcactc
    10261 cggcgcagat cgagtccgat ccggcgtcac tgaccggggc ctatctgtcg ggtcggcaga
    10321 gcgttgcatt gcccgagcgg cggcgcagcc cacagccgaa ccagtggcta tcgatccgag
    10381 gcgcacgcag caacaatctg aagaacatca cggtggacct gccgatcggt ctgatgacct
    10441 gcgtgaccgg cgtgtccggc tccggaaaat ccaccctggt ggaggacaca ctgtttcggc
    10501 acgccgctgt gcgcctgaat ggtgccaatc cggacggtgc cggtgtgtgc gatgagatcg
    10561 tcggactcga gcgcttcgag cgcgtgattg aaatcgatca gaacccgatc ggccgcacac
    10621 cgcgctcgaa ccctgccacc tacaccggtg tgtttacgat cattcgcgaa ctgtttgcct
    10681 cggttcccga agcgcgcgcg cgtggttatg acgccgggcg cttcagcttc aatgtcaaag
    10741 gcggacgctg cgaagcctgt cagggcgacg gcgtgatcca ggtggagatg cacttcctgc
    10801 cggacgtgta cgtgccgtgc gatgtctgtc gcggcaagcg ctacaaccgt gaaacgctgg
    10861 agatccagta ccggggacgc aatgttcacc aggtgctgga cacgacgatc gaggaggcac
    10921 tgcagctgtt cgagaacgtg ccggcagtgg ctggcaagct gcgtacgctg atggatgtcg
    10981 gcctgaccta tgtgcgcctg ggacagaacg cgaccaccct ctccggtggc gaagcgcagc
    11041 gggtcaaact ggcacgcgag ctggccaagc gcggcgccgg ccgcacgctc tacatcctgg
    11101 atgagccgac taccgggctg catttccacg acatcgcgca gctgctgcgc gtgttgcagc
    11161 aactacggga tgcgggcaac accctggtga tcatcgagca caatctcgac gtcatcaaga
    11221 ccgctgacta cgtgatcgat ctcgggccgg aaggcggtga gcgcggtggt cgggtggtgg
    11281 ccagcggtac gccggaagcc atctgcgatc agccggaatc ggtgaccgct caatacctgg
    11341 cgccgctgct gcagcgggtc agcgaacccg ccgcgatccg gactcgaagc gctggtgcac
    11401 ctcgccgttg acgatgcgct cctgatagcg gccgttggtg taggcgcaga ccacgcgccg
    11461 ccagaaactc acggcggtgg cgttgcgcat gtgttcggtg atcagccagc gaccggtaaa
    11521 acgatccagc agcagtcgta ccgcctgcgc gccgatgccc cgccgacgcc actggcgcgc
    11581 gatgaagaat tccttcatcg aatattcgat ggtcggagaa gtcccggcag cgccgccact
    11641 ggcgactgcc tgcaggcgct gttcaaccat cgcgaagccc gcgcgctcca gaccgtagac
    11701 cacggtcatc acgtgcgcgc ttcggtccga ataccagtgc atcaactgat ccggcacgcg
    11761 atgaccgact tcgccaaggg ccggaaagat gccggtcgca gtcggcgcca gatccgtcag
    11821 atagtcgtga tagacgctct cgatccagcg gcgatcgtcg cggttatggc gaccgtccct
    11881 gaggctgacc tgattcatga attcgcttcg gaaacaggaa agcccggcag accggatcct
    11941 tccggcctgc tcgggctttc ccgcgagcct ggaactcgtc ggctttcgaa agacccgaag
    12001 ttacttcggt gccgaaccac ccgaggacgg cgtcgtcgcc gagctgctgc tggtgccgtc
    12061 ggacggcgcc atcgcgccgc tgctgctgcc ttccgtcgca ggagccatgg tagcggacgg
    12121 cgtggttgcc gccggggccg aggcgggcgg gggcgtattg tccgtgctgc tgcaagcaga
    12181 gagcacaagc gcggccgcca gggcgctaag ggcaagcttt gtattcatcg atgaatcccc
    12241 agaattgtat gaaagaagct gaccgaatat gtggagcgca aaccgtttta tcgggcaatg
    12301 agactcccga accgcggatt ctatagagta tccacgccat tttaaactgg cgctgacgcc
    12361 ctatcttgcc tgaacctttc ttgaggccgg tcgctagccg gcagccgctt tcttcttccg
    12421 cggctttgcc ttgggcttct tttcgacctt atccgcagcg tcatcggccg ccttggcctt
    12481 cttcttcggt gcgttcttgg ccttggctgc gggcttgccc ttatcgccct tatcgccctt
    12541 atcgcccttg tcgggcttgg cggccttagc cttgccctta gcctcggtcg ggggcttgac
    12601 ttcctgagcg gccgcaggac cgtccaccag ctgcatcagc gccatcgggg ccgagtcacc
    12661 gggacgcggc agcatgtgca ggatgcgcgt atagccgcct ggacgggctt tgaaacggga
    12721 gcccaggtca tcgaacagct tggtgacgac gtcctgatcg cgcagccgcg aaaaggccag
    12781 acgccgatgc gcctcggtat cgtccttggc gagcgtgatc agcggttcca ccacgcgccg
    12841 cagttctttg gccttcggca acgtcgtgcg gatggtctca taccgcagca gcgaggctgc
    12901 catgttgcgc aacatcgcat gtcgatgcga gctgttacgg gacaattgac ggccagaaag
    12961 cttgtgacgc atgactactc tttacagatc attgaaacag atcattgaaa cagatcattg
    13021 aacccaatcg aacccggcag cgatccaccc tgacgcggcg cccattgcgc gacgccgcca
    13081 ccgctcgtca tcttcagatc aggccgctgc cgctctcttc gtcgtggcgc agaccagccg
    13141 gcggccagcc ctccaggcgc atgccgagca tcaggccgtg gctctgcagc acttccttga
    13201 tctcggtcag tgacttcttg ccgagattcg gcgtgcgcag caactcgact tcggtacgct
    13261 gcaccagatc gccgatgtaa tgaatgttct ccgccttcag acagttcgcg ctgcgaaccg
    13321 tcagctccag ctcatccacc ggacgcagca ggatcggatc gaattgcatc tcggtagtct
    13381 tggccaccgc ctcgtcttcg ccctgcagat cgacgaacac cgacagttga tccttgagaa
    13441 tggcaccggc gcgccggatc gcctcttccg gctcgatcgt gccgttggtc tcgatatcga
    13501 t
//
LOCUS       DATMRM010000181         3225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1680332, whole genome shotgun sequence.
ACCESSION   DATMRM010000181 DATMRM010000000
VERSION     DATMRM010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680332"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="VNZ06_05335"
     CDS             <1..1087
                     /locus_tag="VNZ06_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20207.1"
                     /translation="QVLPAMQIGHFLSLCDDTGLLQHALHCVPDRSHGYCTDDNARAL
                     LVTCRLSRSGDLPLSEPLTSRFAAFIQHAWNRDAKRFRNFLSFDRHWLEDLGSQDSHG
                     RALWALGECTRNAASPSVRRWAASLFAEALSTVEAFGSPRAWSFALLGLDAYCAAAAE
                     DSHARRLRDLLANRLMVRLAETETRGWEWFEASLAYDNARLPQALIATGCATDTASYT
                     SAGLRCLHWLARLQTSASGMFRPVGSQSFGAQRVPPQPFDQQPLEAAAMISACAAAWR
                     ADRDPAWVAEAGRAFAWFLGSNDLSVPLVDPETGSCRDGLHPDRANENRGGESAVSYL
                     LALAEMRELAAFNTERATLVPGRVVNA"
     gene            1113..2393
                     /locus_tag="VNZ06_05340"
     CDS             1113..2393
                     /locus_tag="VNZ06_05340"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HWW20208.1"
                     /translation="MLLNRQALHLRPNPARVVLRPFKPATEPRELNRTDRRRANHIVD
                     RVLALDPETAGRHLAEVLENFQDRHRDLLASFDARAGDMEDVFATHVAFSRTQRQLVG
                     AYFLHEYSFEAAALFNPSIVAHPDQTGVPGGALRFILSLRAVGEGHISSLTFRSGLIA
                     ADGAVTVDPTERLASIPYLQPPELGAPLDCIDVMFKSDEHLSERVIFPITASQSNGIE
                     DARFVRFEDNGRSTFYATYTAYSGKAIRSELIETRDFKSFRMTPLKGVAARNKGMALF
                     PRKLRGKYAMIGRQDNESLYLIYSDDLYSWSEGVALLKPQFPWEFVQIGNCGSPIELE
                     QGWLLFTHGVGPVRRYSIGAALFDKADPSRLLGRCTEPLLRPELSEREGYVPNVVYTC
                     GAMRHNDHIILPYAVSDTYCCFATIETAALIKSM"
     gene            complement(2421..2648)
                     /locus_tag="VNZ06_05345"
     CDS             complement(2421..2648)
                     /locus_tag="VNZ06_05345"
                     /inference="COORDINATES: protein motif:HMM:NF023507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3562 domain-containing protein"
                     /protein_id="HWW20209.1"
                     /translation="MNTSDAAILAQEHRAIERLAHDTHTSVAKVQQVFLSEYAKLATD
                     AYIRSFLPLLVANRVRALLHGRRAVPMALPH"
     gene            2824..>3225
                     /locus_tag="VNZ06_05350"
     CDS             2824..>3225
                     /locus_tag="VNZ06_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20210.1"
                     /translation="MTDSTPASNWVLASIPSRQCERLRSQLKPVTLEFGQVLYEPGGT
                     IKHVYFPIDCLISLLTAVDRGRTLEVGMVGNEGMAGMPFILGMGVSGVRALVQGGGGA
                     LRIASAPFRAEFARNMALQQALYRYTYALMAQ"
BASE COUNT          585 a         1012 c         1010 g          618 t
ORIGIN      
        1 acaggttctg cctgccatgc agatcggaca cttcctgtcc ctgtgtgatg acaccggctt
       61 gctgcaacac gcgctgcatt gcgtgcctga ccgatcgcat gggtactgca cagacgacaa
      121 cgcgcgcgca ttgctggtga cctgtcgcct cagccgatct ggcgatctgc cactgtccga
      181 accattgacg agccgcttcg cggcctttat ccagcatgcc tggaatcgcg atgcgaagcg
      241 gtttcggaac ttcctgagct ttgatcgaca ctggctcgag gatctgggat cgcaggacag
      301 ccacggtcgc gcgctctggg ccctgggcga atgtacgcgc aacgccgcca gcccttcggt
      361 gcgtcgatgg gccgcgtcat tgtttgccga ggcgctgtcg accgtggagg ccttcggttc
      421 accgcgggca tggagtttcg cgttactcgg attggacgcc tattgcgcgg cggctgcgga
      481 agactcacac gccaggcgtc tgcgagacct gctggccaac aggctgatgg tcaggctcgc
      541 ggaaaccgag acccgcggtt gggagtggtt cgaagccagt ctggcctatg acaacgcgcg
      601 tttgccacag gccctgatcg cgaccggatg tgcgaccgat acggcctcct ataccagtgc
      661 gggtctgcgc tgcctgcact ggctcgcgcg ccttcagacc tctgcaagcg gaatgttcag
      721 gccggtcggc tctcaaagct tcggcgcgca acgcgtgccg ccgcagccct tcgaccagca
      781 gccgctggag gctgcagcga tgatctcggc gtgtgcggcc gcgtggcgag cagaccgcga
      841 cccggcgtgg gtcgccgagg cgggacgggc tttcgcatgg tttctaggca gcaatgatct
      901 gtcagtgcca ctggttgatc cggagacggg cagctgccgt gatgggctgc atcccgatcg
      961 cgccaacgag aaccgaggcg gggagtccgc tgtttcctac ctgctcgctc tggcggagat
     1021 gcgtgagctc gccgccttca atactgagcg ggcaacgctc gtgccaggtc gggtcgtcaa
     1081 cgcctgacac accaaaggat ctcccgacag tcatgctgct gaaccgccaa gccctgcatc
     1141 tgcgtccaaa cccggcaagg gtggtgctgc gccccttcaa gcccgcgacc gagccgcgag
     1201 agctcaaccg cacggatcgc aggcgcgcca atcacattgt cgatcgggta ctggcactgg
     1261 atcctgagac cgccgggcgc catctggccg aagtgctgga gaatttccag gatcggcatc
     1321 gcgatctgct ggccagtttc gacgcgcgtg ccggcgacat ggaggatgtc ttcgcaacgc
     1381 acgtcgcctt cagccgaacc cagcggcaac tggtcggcgc ctacttcctg cacgagtact
     1441 ccttcgaagc agcggcgctg ttcaatccca gtatcgtcgc tcatccggat cagacgggtg
     1501 tacccggcgg cgcacttcgc ttcatcctca gtctgcgggc ggtcggcgaa ggccatatct
     1561 cttcgctcac atttcgctcg ggcctcatcg ccgccgatgg cgccgtcacg gtggacccga
     1621 ctgagcgcct ggcttcgata ccgtacctgc agccgcctga actcggggca ccgctggact
     1681 gcatcgatgt gatgttcaag tcagacgagc acctcagcga gcgcgtgatc tttccgatca
     1741 ctgcgtcgca atccaatggc atcgaagatg cccgcttcgt cagattcgag gacaacggcc
     1801 gcagcacgtt ctacgcgacc tataccgctt acagcggcaa agccatccga tcggagctga
     1861 tcgagacccg ggacttcaaa tccttccgga tgacgccact gaaaggcgtg gcggcgcgca
     1921 acaaaggcat ggcgctgttt ccgagaaaac tccgcggcaa gtacgccatg atcgggcggc
     1981 aggacaacga gagcctctac ctcatctact ccgatgatct gtattcctgg agtgagggcg
     2041 tggcgctgct caagccgcaa ttcccgtggg aattcgtaca gatcggcaat tgcggctcgc
     2101 cgatcgagct ggagcagggc tggttgctgt tcacgcacgg agtgggcccg gtcagaagat
     2161 attcgatcgg cgcggcactg tttgacaaag ccgatccatc caggcttctg gggcgctgca
     2221 ccgaaccgct gctgaggccg gaactatccg agcgcgaggg ctacgtaccg aacgtggtct
     2281 atacctgcgg cgccatgcgg cacaacgatc acatcattct gccctatgcg gtgtccgaca
     2341 cgtattgctg cttcgccacg atcgagacgg ccgcgctcat caaatcgatg tagcagaccc
     2401 ggcctgccgg gcagttctct tcagtgcggg agtgccatcg ggaccgcgcg gcggccgtgt
     2461 aacagggctc tcacacggtt agccacgagt agcggcagga acgacctgat gtacgcatcg
     2521 gtcgcgagct tggcatactc actcaagaat acctgctgta ccttcgcgac gctcgtgtgg
     2581 gtgtcatgag ccaggcgttc gatggcacgg tgctcctgcg ccaggattgc tgcatccgac
     2641 gtgttcattg aatcggtctc gttgctagag cacgcagtct ggatgtcaac ccagctgacg
     2701 tcggtgcggc agcagacgca gcgcacgaac gctcatcgat ggccgagata cgttcgccgc
     2761 cgcacataca tggacgcgcc tgatctccat actgtcgggc ctgtggtcct gaagaacgcc
     2821 caaatgactg actcaacacc ggccagcaac tgggtgctgg cgagcatccc gagcaggcag
     2881 tgcgagcgcc tgcgctcgca actcaagccg gtgacactgg agtttggaca ggtgctctat
     2941 gaacccgggg gcaccatcaa gcatgtctac tttccgatcg actgcttgat ctccctgctg
     3001 acggcggttg accgaggcag gacacttgaa gtgggcatgg ttggcaacga gggtatggcg
     3061 gggatgccct tcatcttggg gatgggtgtg tccggcgtgc gcgcactggt gcagggtggc
     3121 ggcggtgcgc tgcgaatcgc atccgcgccc tttcgggccg aattcgctcg caacatggcg
     3181 ttgcagcagg cactctaccg atatacctac gcgctgatgg cgcag
//
LOCUS       DATMRM010000182         4448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2894032, whole genome shotgun sequence.
ACCESSION   DATMRM010000182 DATMRM010000000
VERSION     DATMRM010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2894032"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="VNZ06_05355"
     CDS             complement(<1..335)
                     /locus_tag="VNZ06_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWW20211.1"
                     /translation="MRPLKNLCEVAVIGGGLAGLAAARHAARLGLLVSLFEGSGLFGG
                     LVATIDEVDGIPVPGKFSGQDLAMHLLADARRAGVRVVETAVTKIELEQDLTLSDQKD
                     GMYHPEAII"
     gene            complement(332..2239)
                     /gene="selB"
                     /locus_tag="VNZ06_05360"
     CDS             complement(332..2239)
                     /gene="selB"
                     /locus_tag="VNZ06_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HWW20212.1"
                     /translation="MTRLVLGVIGHVDHGKTTLVRALTGQDTDRLVQEKQRGISIALG
                     FANLTIGSDTHIDLIDMPGHERFVRTMIGGATGIDAVLLVVAANEGIKPQTIEHVDIA
                     SLLGLRRAVVAITKTDLVASEQVQRVAQETVRLLAQSGLDPLPPAKTSLPSEHGIEDL
                     REALKVLAAGQSPRSADGLAFLPIDRAFSIAGHGPVVTGTLRGTAVTTGDTLELSPLR
                     RLVRVRAVQVHGSRVDAAAPGQRVALNLRDVDIAELKRSMALATPDSLQLSNWLTISI
                     RAVDGAPPLKNGIRLRTLLGTGEYDSRLRLLDREVLEAGQSGLAQLHFAEQIALPAGE
                     HVLLRLPSPPRTVAGGKVLDPGAQRVRRHCSAVLEHLEDLRVLPPAAMIAAEVRRQGV
                     SGTTLQRLSQLSALATPRVIELLHPLSIVVTRSGLVVQQAHVDAVISRIPSLLAPHAT
                     GLSRDKLVSALGITAVVLDEALGRLRARGIIRERSGLFCLPRPDEESARDRQEAALAG
                     HIAETLRQAGLTPPNPGAIVTDSKSKRAVERLLRAGVVVRAVDRAKGREILFHRDAID
                     DAQRRLAPLLDRVPGMLVTEIGAALGISRRYNMPLLDHLDTIGFTRRIKDRRIRADPA
                     RAKWVARDYLG"
     gene            complement(2257..3093)
                     /locus_tag="VNZ06_05365"
     CDS             complement(2257..3093)
                     /locus_tag="VNZ06_05365"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HWW20213.1"
                     /translation="MNLRIEPVKSAIGAVVHVDRSSLTDEGVAARCLELLEKHGALIF
                     PRLGLTADEQLAFTDQLGARVNFTKTAPGGDKDGAGVYTITLDPKVNNEPEYVLGTFF
                     WHMDGITLANIPPPKASVLTCRRTAPKGGQTEFASTYAAYEALPEEEKAELNGLRVMH
                     SVTAAVREVAAPEEIEPFKRGLKAEHPLVWTHKSGRKSLLIGYTADYVLDMPKAAGRA
                     LLARLLEWTAQPAFSYRHKWQEGDLVVWDNCGALHRVIPYAADSGRTMHRTSVAGIEA
                     VT"
     gene            complement(3226..3855)
                     /locus_tag="VNZ06_05370"
     CDS             complement(3226..3855)
                     /locus_tag="VNZ06_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWW20214.1"
                     /translation="MTRPDLQESEGTDAGSVRPRRMGVEGSKTRTLFVDAAEAILRDE
                     GYRGISARQVSSKAGLKTQLLYYYFRKMDDLILAVVRRINERRMTRFEEALASSDPLR
                     ALWEASSDPSSAALSAELTSIASHREAIRAEIVRAAQQFRSLQIQAVSRLLAHRNEED
                     YPAAGIVMIAVALARTLVTESALGLTEGHHEALAIVQQMLKHFGSSGRR"
     gene            complement(3860..>4448)
                     /locus_tag="VNZ06_05375"
     CDS             complement(3860..>4448)
                     /locus_tag="VNZ06_05375"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20215.1"
                     /translation="ADLGSGLLRETPGTSEPSLTDALSAGADLITCSGDKLLGGPQAG
                     LILGRKAVVDPLRHHPLLRAVRLDKMSLAALETTLMLHRDEPGRIPVRYLLGQSEAQL
                     QQRAERLQVMLGSGTIERTEAFAGGGSLPEERIMSRAVALHLKMGAEEAADLLRACHP
                     AVIGRIKEGRLLLDVLTVFDSDLPELAAALRAVLS"
BASE COUNT          826 a         1438 c         1356 g          828 t
ORIGIN      
        1 atgatgatgg cctcgggatg atacatccca tccttctgat cgctaagcgt gaggtcctgc
       61 tccaactcga tcttggtgac cgcggtttca acgacgcgca cgcctgccct gcgggcgtcg
      121 gccaacaaat gcatggccag atcctggcca gagaatttcc cggggacggg gatcccgtca
      181 acctcgtcaa tcgtggcgac cagtccacca aagagtccac tgccctcgaa cagcgacacc
      241 agcaggccca gacgcgcagc gtgacgcgcg gccgcgagtc cagccagacc gccaccaatg
      301 acggcgactt cacacaggtt cttgagcggc ctcatcctag gtagtccctt gcaacccatt
      361 tggctctggc ggggtcagcg cggatccgac gatccttgat tcggcgagtg aaaccgatcg
      421 tgtcgagatg atccagtagc ggcatgttat accggcgtga gatgcccagt gctgctccga
      481 tctctgttac cagcatgccc ggtacacgat ccagcagagg cgccagtcgg cgctgcgcgt
      541 cgtcgatggc gtcccgatga aacaggattt cccgaccctt cgcacgatca acggcacgta
      601 ccacgacacc tgcgcgcagc agacgctcca ccgcgcgctt tgatttcgaa tccgtcacga
      661 tcgcgccagg atttggcggc gtgagtcctg cttgccgcaa agtctcggct atgtgaccgg
      721 caagcgctgc ctcctggcga tcacgagcgg attcctcgtc tggccgggga agacagaaca
      781 ggccgctgcg ttcgcggatg attccgcgcg cgcgcagtcg cccgagtgcc tcgtccagca
      841 ccacagcagt gatccccaag gcagagacta atttgtcgcg cgagagccct gtcgcgtgtg
      901 gcgcgagcag cgaggggata cgcgaaatca cggcgtccac gtgggcttgc tgcaccacca
      961 gccctgagcg cgtgaccacg atcgagagtg gatgaagcag ctcgatgacc cgcggtgttg
     1021 cgagtgcgct gagctgcgag aggcgctgca aggtggtccc agacactcct tggcgccgca
     1081 cctcggcagc aatcatcgca gcgggcggca gtacgcgcag atcttcaaga tgctccagaa
     1141 ccgctgagca atgacgtcgc acgcgttgcg ctccaggatc cagaaccttg ccgccagcga
     1201 ctgttctggg cggagagggt agacgcagca gcacatgctc gcccgccggc agcgcgatct
     1261 gctcggcgaa gtggagctgg gcaagaccgc tctggcccgc ttcaagcacc tctcggtcca
     1321 gcagccgcag gcgcgagtcg tattctccgg tgccaagcaa tgtgcgaagc ctgatcccgt
     1381 tcttcagcgg tggtgcccca tcgacagcac gaatcgaaat cgtgagccag ttcgagagct
     1441 gcagcgagtc aggcgtggcc agagccatgc tgcgtttcaa ttcggcgatg tcgacgtcac
     1501 gcaggttcag cgccacacgc tgcccgggtg cagcggcgtc gacgcgcgat ccatgaacct
     1561 ggacagccct tacgcgcacg agccggcgca acggcgacaa ctccagcgtg tcgccagtcg
     1621 tgacggctgt cccgcgcagg gttccggtca caaccgggcc gtggccggcg atgctgaatg
     1681 cgcggtcgat tggcagaaat gccaagccgt cggcgctgcg cggtgactgg cctgcggcca
     1741 ggactttgag ggcttcacgc aagtcttcaa tgccgtgttc ggatggcagc gaggttttcg
     1801 cgggtggcag cggatccaga cccgattggg ccagcagacg aacagtttcc tgtgcgaccc
     1861 gttggacttg ctcggacgcg accagatcgg tcttggtgat cgcgaccaca gcgcgacgca
     1921 atcccagcaa actggcaatg tcgacgtgct cgatcgtttg cggtttgatg ccttcgttag
     1981 ctgccacgac cagcaggacc gcgtcgatcc ccgttgcccc accgatcatg gtacgcacga
     2041 aacgctcgtg accaggcatg tcgatcagat cgatgtgggt atccgaccct atcgtcaggt
     2101 tcgcgaaacc gagcgcgatg gagatcccgc gctgcttctc ctgcacaagg cggtcggtgt
     2161 cctggcctgt caacgctcga accaacgtgg tcttgccgtg atcgacgtga ccgatcacgc
     2221 ccagcacgag acgcgtcacg ctcaggcgcc gcggccttaa gtcactgcct caatgcccgc
     2281 aacggacgtg cggtgcatcg tgcggccaga gtcggcggcg taagggatga cgcggtgaag
     2341 tgctccgcag ttgtcccaga cgaccaggtc tccttcctgc cacttgtggc gataggaaaa
     2401 agccggctgg gcggtccact ccagcaatcg cgcgagtagc gctcgcccgg cggccttcgg
     2461 catatccagc acgtagtcgg cggtgtagcc gatcagcagc gacttgcggc cggacttgtg
     2521 ggtccagacc aggggatgct cggctttgag cccgcgtttg aacggttcga tctcctccgg
     2581 ggcagcgacc tcgcgcacgg ccgcggtcac gctgtgcatc acgcgcaacc cattgagttc
     2641 cgccttctcc tcttctggca acgcctcata cgcggcgtac gtactcgcaa actcggtctg
     2701 gccgcctttc ggcgcagtgc gccggcaagt cagtacgctc gccttcggcg gcggaatatt
     2761 tgccaaggtg atcccatcca tgtgccagaa gaaggtgccg agtacgtatt ccggctcgtt
     2821 attgaccttc ggatccagag tgatggtgta gacgcccgcg ccgtccttgt cgccgccggg
     2881 cgcggtcttc gtgaaattca ctcgcgcccc aagttggtcg gtaaaggcaa gctgttcgtc
     2941 agcggttagg ccgaggcgag ggaagatcag cgcgccgtgt ttttccagca gctccaggca
     3001 gcgcgcagcg acgccctcgt cggtcagcga agagcgatcg acatgtacga cggcccctat
     3061 ggccgactta accggttcga ttcttaggtt cacggcgaca actcccctag ccctatcagg
     3121 gttaaccgcc cccggcaccc cagattatgt tgaacaggtt tcgggtgttc ggcagcggca
     3181 ggttcaaatg ctgaacacca ccttagcatt gaaatttgcc tcgggtcaac gacgacccga
     3241 actgccgaag tgcttgagca tttgctgcac gatcgcgagc gcttcgtgat gcccttcggt
     3301 gaggcccaaa gcggactccg tcacgagggt tcgcgccaga gcgaccgcaa tcatgacgat
     3361 tcctgccgcg ggatagtcct cttcgttgcg gtgtgcgagc agccgagaca cggcctggat
     3421 ctggagtgag cgaaactgct gagccgcacg gacgatctcg gcgcgtatcg cctcgcgatg
     3481 gctggcgatc gaagtcagtt cggcagacaa ggcggcgctg gacggatcgc tgctggcttc
     3541 ccacagtgcc cgaagcgggt cggaggaggc caatgcctcc tcgaatcggg tcatacggcg
     3601 ctcgttgatg cgccggacga ccgcaaggat cagatcgtcc atcttccgga agtagtagta
     3661 gaggagctga gtcttcagcc ccgccttgct cgagacctgc cgtgctgaga ttccacgata
     3721 tccctcatcg cgcagaatcg cctcggctgc gtcgacgaac agtgtgcgcg tcttcgagcc
     3781 ttccacgccc attcggcggg gtcgcacgct accggcatcc gtgccttcgg actcctgcag
     3841 gtctggccgc gtcatcgcgt cagctcagta ccgctcgaag tgccgcggca agttcaggta
     3901 gatcgctatc gaatacagtc aacacatcga gcaacagacg tccctctttg atgcggccga
     3961 tcacggccgg atgacaagcc cgcagaagat cggccgcttc ttcggcgccc attttcaaat
     4021 gcagcgctac cgcccggctc atgattcgct cctccggtaa cgagccgccg ccggcgaacg
     4081 cctccgtcct ctcaatcgtg ccgctaccga gcatcacctg caaacgctcg gcgcgttgct
     4141 gcagctgagc ctcgctctga ccaagcaggt atcggactgg gattcgccca ggctcgtctc
     4201 gatgcaacat cagcgtcgtc tccagtgctg ccagcgacat cttgtccagt cgaaccgccc
     4261 gcagcagggg atgatgacgc aatggatcga ctaccgcttt tcggcctagt atcagtccgg
     4321 cctgtggtcc tcccagcaac ttgtcgccgc tgcaagtgat caggtcggcg ccagccgaca
     4381 gcgcatcagt caatgaaggc tcgctcgtgc cgggtgtctc acgcagcaat ccgctgccca
     4441 gatccgcc
//
LOCUS       DATMRM010000183        12101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4136773, whole genome shotgun sequence.
ACCESSION   DATMRM010000183 DATMRM010000000
VERSION     DATMRM010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12101
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4136773"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..510
                     /locus_tag="VNZ06_05380"
     CDS             76..510
                     /locus_tag="VNZ06_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20216.1"
                     /translation="MRIAVATFSSLLLVCSVSMAQNPVQQVKQDNADIRQLNTDVRNN
                     AADVRKDSADVRYDQTDINRDRLTRSRDQQRENQDLARGDVKGAQYWNKQRLNENGEI
                     KHDERNLAHSRLDVKNAKARLSKDVAVRHNDVVKRNRAAGKI"
     gene            566..928
                     /locus_tag="VNZ06_05385"
     CDS             566..928
                     /locus_tag="VNZ06_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20217.1"
                     /translation="MRTTVPLMLAAAIGLAASLHAPFAKAGGLYVGLQVPTLVTAPLV
                     TEVPVAPVAIVTDDDPTFIVTSGDCCWHGDDWHRHGRYEDGWHSGGWRGVRGRSAYEH
                     RGNAPGPRGHEVARSGRR"
     gene            complement(1040..1112)
                     /locus_tag="VNZ06_05390"
     tRNA            complement(1040..1112)
                     /locus_tag="VNZ06_05390"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Thr,seq:cgt)
     gene            complement(1165..2307)
                     /locus_tag="VNZ06_05395"
     CDS             complement(1165..2307)
                     /locus_tag="VNZ06_05395"
                     /inference="COORDINATES: protein motif:HMM:NF005313.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWW20218.1"
                     /translation="MQGVTLVANTRRALVVGGSVGGMASAISLQRAGFQVDLAEIDPA
                     WMIYGAGLTITAPTLRALQVLGVLDEVTQQGATWELISVFTRDGERIQELRTPRISPE
                     LPAHGGIMRPVLHRILSSATRKAGVDVRLGTTLETMTQVPRGVEVKLTDGHRACYEIV
                     VGADGIYSKLRERIFPDAPKPTFTGQVIYRVVAERPQGFDRGEFYRSEDAQLGFNPVS
                     ATHLYMYLLERQPANPWMPLEEQPRRLYQSLEGWGGIVPRVRAVVMTSSAPTVNYRPL
                     EAMLLPRPWFQGRVVLIGDAAHAATPHLASGVGMAIEDALVLADELKNGEIDVALPRF
                     MDRRFDRSRMAVENSVRIGEIEMKHGSPDEQGRLLAESMQALRQPM"
     gene            complement(2355..2801)
                     /locus_tag="VNZ06_05400"
     CDS             complement(2355..2801)
                     /locus_tag="VNZ06_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HWW20219.1"
                     /translation="MDSQAALTESNWAKVATRLADCGIRVTDQRLQIATILLSAPQHL
                     TAEQIADALRHGGARVSKATVYNTLNLFAARGLIRQLSVDGTCAWFDSNVEPHYHFHD
                     EHSGALSDVALHDVQFSRLPSPPVGMEVAGIDLVIRLRRRNSSKPS"
     gene            complement(2937..3875)
                     /gene="ispH"
                     /locus_tag="VNZ06_05405"
                     /gene_synonym="lytB"
     CDS             complement(2937..3875)
                     /gene="ispH"
                     /locus_tag="VNZ06_05405"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017907393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWW20220.1"
                     /translation="MRIILANPRGFCAGVDRAIEIVERALQMFGTPIYVRHEVVHNHH
                     VVARLRDLGAVFVEELSEVPDGATVIFSAHGVSTAVEEEALRRGLQVFDATCPLVTKV
                     HMEVSRFAREAREVILIGHAGHPEVEGTMGRFDTSFGGHMYLVQGIEDVKTLRVRDTQ
                     NLAFVTQTTLSVDDTAAIVETLRARFPSLQAPRREDICYATQNRQDAVKQMIEQCDAL
                     IVVGSKSSSNSNRLRELAERAGVPGYLVDGPDDMDRGWVDGKDNVGVTAGASAPEVQV
                     QAVVQRLRSWGGQVPSELSGREEHVVFGLPKALRAQ"
     gene            complement(3885..4400)
                     /gene="lspA"
                     /locus_tag="VNZ06_05410"
     CDS             complement(3885..4400)
                     /gene="lspA"
                     /locus_tag="VNZ06_05410"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019486629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HWW20221.1"
                     /translation="MAQSDFGGETTIVAGARAGGLIYLWLSIAVIALDQLTKTLIERW
                     LGLYQSIPVLPVLEITRLHNTGAAFSFLADEAGWQRWLFTALAIVVSVVLVLWLRRID
                     RGAKLLASAVALILGGALGNVIDRLRLGHVIDFVHAHWGEHYFPAFNVADSAITIGAA
                     MLLLDAWLEGR"
     gene            complement(4400..7231)
                     /gene="ileS"
                     /locus_tag="VNZ06_05415"
     CDS             complement(4400..7231)
                     /gene="ileS"
                     /locus_tag="VNZ06_05415"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HWW20222.1"
                     /translation="MDYKKDTINLPQTDFPMKADLAQREPAQLKRWLDEDIYARIREA
                     SRGRPAFRYHDGPPYANGVIHLGHAVNKILKDIVVKSQTMDGRDAAFIPGWDCHGLPI
                     ELAVEKKHGKPGHKLDATAFRAACRAFANQQIDSQRADFKRLGVFADWDNPYLTMDPR
                     YEAQQIRALGKIIRNGHLYRGAKPVHWCLDCRSALAEAEVEYEDRTSTAIDVAFPVVD
                     RGDFAKRIGVAREALKGPVSVVIWTTTPWTLMANEAVSVGPDFNYVAVAVTRNSQREV
                     LVLASQLKDSCLARYGLTAIEQLAEFPGRVLERLQLSHPFLPRQSLLILGEHVTLDAG
                     TGAVHTAPAHGQEDFLVAQRYQLPVANPVGPDGKYLPDTALVAGLRVDKAEAVIIAQL
                     IEKAALLHQEPLRHSYPHCWRHKSPLIFRATPQWFISMDAHKLREHALRDIRQVRWTP
                     AWGEQRITSMIEHRPDWCISRQRTWGVPLALFVHKQTAALHPRTQELLLQVAQRVESG
                     GIDAWFALDAKELLGADAEHYEKLTDVMDVWADSGLSFECVAAIRPDFSAPVDLYLEG
                     SDQHRGWFHSSLLMSEALYERAPYRGVLTHGFTVDDKGRKMSKSLGNGIEPQDIMRTL
                     GADILRLWIGATDYSGEMSLSQEILRRLTDSYRRMRNTVRFLLGNLSDFDPQQSVPIS
                     QLVAIDRWAIARTRALHDNVVQSYRDYQFHQIYQQVHNFCVVDLGGFYLDILKDRLYT
                     TPASSHARRSAQTAMWHIGESMVRWLAPMLSFTAEEMWGFLRGQRPQSVFLTTWHELP
                     QVPDDEIDWTALIALRADVARELERLRVSGAIGAPLDAELEVWCTAPQYRRLSRLGAE
                     LHFLMITSEATVHEVALNDRAAVPVDAVAAPSVDGGEVWIRVRASTHTKCIRCWHHRP
                     DVGQVPEHPQICLRCVGNLTLPGEARRYS"
     gene            complement(7231..8148)
                     /locus_tag="VNZ06_05420"
     CDS             complement(7231..8148)
                     /locus_tag="VNZ06_05420"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HWW20223.1"
                     /translation="MELIRRLDSNSAHDCVVTIGGFDGIHLGHHALIERLCAHGTRLG
                     LPTMLVSFEPLPREYLQPADPPPRLTNFRERWRLLSSTGLERLCVLTFSERLRTLTGL
                     QFMQLLQAAHARIVVVGHDFRFGMGGEASAQWCAERGSQFGFEVDVISPVPIDGLRVS
                     SGQVRDAVRVGDFAHARRLLGRPYGMRGRVRCGNRLGRTLGFPTANVAVKRRRVPLSG
                     VFAVRAHGAGLSAWPGVANLGTRPMVGGTHMLLEVHLFDFSGDLYGRELEVEFVARLR
                     DEASFASMDAMVQQMHRDAAQARRQLGER"
     gene            8372..8644
                     /gene="rpsT"
                     /locus_tag="VNZ06_05425"
     CDS             8372..8644
                     /gene="rpsT"
                     /locus_tag="VNZ06_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HWW20224.1"
                     /translation="MANTKSAEKAARQAAEHRTHNVTLRSRMRSAIRKVNDAVASGNK
                     DDAQKSYQAAVPVIDSMVNKKILHRNAAARYKSRLATRVKSVGATR"
     gene            complement(8675..9001)
                     /locus_tag="VNZ06_05430"
     CDS             complement(8675..9001)
                     /locus_tag="VNZ06_05430"
                     /inference="COORDINATES: protein motif:HMM:NF016855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HWW20225.1"
                     /translation="MRNSKVRARIAAGKLRLRIRDLRNLGTKSEEMLSEIGVHSADEL
                     RRRGAVEAFYQLKLAGVTSSLNTLWALAGALEPWPEGRHWREVARGDDRLSLLLALED
                     RERGRS"
     gene            complement(8994..10040)
                     /gene="obgE"
                     /locus_tag="VNZ06_05435"
     CDS             complement(8994..10040)
                     /gene="obgE"
                     /locus_tag="VNZ06_05435"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWW20226.1"
                     /translation="MKFVDEATMRVQAGNGGRGSASFRREKFVPFGGPDGGDGGDGGS
                     VWLRSKEGINTLADFRIERVFKATSGDGGSGNDCTGRSGEDLYVAVPVGTQVRDAQTD
                     EALGDLATAGQELKVAQGGKGGWGNTRFKSSTNRAPRQFGPGLPGEKRTLALELKVIA
                     DVGLLGLPNAGKSTLIRAVSAARPKVADYPFTTLYPNLGVVNCGQHRSFVMADIPGLI
                     EGAADGAGLGIRFLKHLQRTRVLLHLIDILPPDPDADPVRDARAIVGELKKFDQELAA
                     KPRWLVVNKRDLLPDEEAEKRAADIARRLRYRGPRFLISAVTGRGTRELAEAVMQFLE
                     TRINAGAADPGSDA"
     gene            complement(10124..10411)
                     /gene="rpmA"
                     /locus_tag="VNZ06_05440"
     CDS             complement(10124..10411)
                     /gene="rpmA"
                     /locus_tag="VNZ06_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HWW20227.1"
                     /translation="MAHKKAGGSTRNGRESHSKRLGVKRYGGQHVLAGNILVRQRGTK
                     VRAGENVGTGTDHTLFAEITGQVEFRIRGAEQRMYVSVQPQAPAANSDKPN"
     gene            complement(10430..10744)
                     /gene="rplU"
                     /locus_tag="VNZ06_05445"
     CDS             complement(10430..10744)
                     /gene="rplU"
                     /locus_tag="VNZ06_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HWW20228.1"
                     /translation="MYAVIATGGKQYRVEKGAVLRIEKLEGEPGSTVEFGEVLLIADG
                     DDVRLGKPQLQGGKVTATIEKHAKGDKVTIVKFRRRKHYMRQKTHRQHYTQVRVTDIS
                     AP"
     gene            10940..11890
                     /locus_tag="VNZ06_05450"
     CDS             10940..11890
                     /locus_tag="VNZ06_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWW20229.1"
                     /translation="MRALVRTELTAVDEAIRSGLHSAVGLVDQIADHIIGGGGKRLRP
                     LLVVLAGRACGGTGTECVRAAAFIEFIHTATLLHDDVVDMSARRRGRDTANEIFGNQA
                     SVLVGDFVYSRAFQLMAATRSQRVIEIMADATNLIAEGEVLQLMNAHDPETTEARYLE
                     VIHRKTAQLFQAGAEVAAVISGAPERVQRELSSYGKHLGTAYQLIDDVLDYQSDPETR
                     GKNLGDDLAEGKPTLPLIYALREGGRCSELMREVIRDGGRERLEDIVGAIESAGGLEY
                     TARLAQSEADEALTALTALPDTPYREALAALVRFAVARKH"
     gene            11904..11980
                     /locus_tag="VNZ06_05455"
     tRNA            11904..11980
                     /locus_tag="VNZ06_05455"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11938..11940,aa:Pro,seq:cgg)
BASE COUNT         2296 a         3942 c         3704 g         2159 t
ORIGIN      
        1 catatccggt caaggacgca agagtccgtt tagctcaacc acccaatttt tcataccact
       61 ctaggagatt ttgccatgcg tatcgcagtc gccacctttt ccagcctgtt gttggtctgc
      121 tctgtctcga tggcgcagaa ccccgttcag caggtgaaac aggacaacgc cgacattcgt
      181 cagctgaaca cggatgttcg caacaacgcg gccgacgtcc gcaaggactc agccgatgtc
      241 cgctacgatc agaccgacat caaccgcgac cgcctcacgc gttcgcgcga ccagcagcgc
      301 gaaaatcagg atctggcgcg tggcgacgtg aagggcgcgc agtactggaa caaacagcgg
      361 ctgaacgaga acggcgagat caagcacgac gagaggaatc ttgcgcacag ccgtctcgac
      421 gtcaagaacg cgaaggcgcg cctgtcgaag gacgtcgccg ttcgtcacaa cgacgtcgtc
      481 aagcgcaacc gggctgccgg caagatctga gtaagccgag atctacccat cactcaccct
      541 gtacttgcta aggagcaacc aagtcatgcg tacgactgtt ccattgatgc tcgctgctgc
      601 catcggattg gccgccagcc ttcacgcacc cttcgccaag gccggcggac tctacgttgg
      661 cctccaggtg ccaaccctag tgaccgcacc gctcgtcacc gaagttccgg tcgcgccggt
      721 ggcgatcgtg accgacgacg accccacgtt catcgtcacc tccggcgact gctgttggca
      781 cggcgatgac tggcaccgtc acggtcggta cgaggacggc tggcacagcg gtggctggcg
      841 aggtgtccgc gggcgttcgg cctacgagca ccggggcaac gcgcccggtc cgcgcggcca
      901 tgaagtcgcg cgatccggtc ggcgctgatc gtcgatccca cgctggcgct ctcgcaacgg
      961 tgcgggagcg cctggtgcgc ctgcatcact gcaacgccgg atgtctacga cgccgcggaa
     1021 tccattttca taagggatat gccggagaga ggactcgaac ctccgacccg cgcattacga
     1081 atgcgctgct ctaccaactg agctactccg gcgctggagg tcgcgtatgt taagcaccga
     1141 cgccgggcag cgccagtagg cgcgtcacat cggttgtcgc agcgcctgca tggactcggc
     1201 cagcagtcgg ccctgctcgt cgggcgagcc gtgtttcatc tcgatttcac cgattcggac
     1261 cgagttttcg accgccatgc ggctgcggtc gaaccggcgg tccatgaagc gcggcaacgc
     1321 cacgtcgatc tcgccgttct tgagttcatc cgccagcacc agagcgtcct cgatcgccat
     1381 tccgacccca gaggccaagt gcggggtcgc cgcgtgcgcg gcatcgccga tcagcaccac
     1441 gcgcccctgg aaccagggcc tgggcaggag catcgcctcg agcggacggt aattgaccgt
     1501 cggggcggag ctggtcatga cgaccgcgcg cactcggggc acgatcccgc cccaaccttc
     1561 gagcgactga tacagccgtc gcggttgctc ttcgagcggc atccacggat tggccggctg
     1621 gcgctccagc agatacatgt acaaatgagt ggccgagacc ggattgaacc caagctgggc
     1681 atcctcgctc ctataaaatt cgccgcggtc gaacccttgt gggcgctcgg cgacgacgcg
     1741 gtaaatcacc tgaccggtga acgtaggctt cggtgcgtcc ggaaaaatgc gctcgcgtag
     1801 tttggagtag atgccatcgg cgccgacgac gatctcatag caggcgcgat gaccgtcagt
     1861 gagtttgact tcgacgccgc gcggaacctg cgtcatcgtc tcgagtgtgg tcccgagtcg
     1921 gacgtccacg cccgccttgc gcgtcgcact ggagagaatc cggtgcagca ccggccgcat
     1981 gatgccgcca tgggccggca gctccggaga gattctggga gtgcgcagtt cctgaatgcg
     2041 ctcgccatcg cgcgtgaata ccgagatgag ctcccaggtt gcgccttgct gcgtgacctc
     2101 gtccagcaca cccaggactt gcagcgcccg cagcgtcggt gcggtgatgg taaggcccgc
     2161 gccgtagatc atccatgctg gatcgatctc agccagatca acctgaaatc cggcgcgctg
     2221 caacgagatc gcagacgcca ttccgccgac actgccgccc acgacaagcg ctcttcgggt
     2281 attggccacc agtgtgaccc cctgcatcga tgttctcgcc gcattgtcag cgagcgatca
     2341 acgatttgtc gagtctatga gggcttggaa gagttccgcc ggcgcagtcg gatcaccagg
     2401 tcgatgcctg cgacttccat accgacgggc ggcgacggta agcggctgaa ctgtacatcg
     2461 tggagcgcga catcgctcaa agcgccgcta tgctcatcgt gaaaatgata gtgcggctcg
     2521 acgttggagt cgaaccaggc gcaggtgccg tcgacgctga gctgacgaat caatccgcgc
     2581 gcggcaaaca gattcagcgt gttgtagacg gtcgctttcg aaacgcgcgc gccaccgtga
     2641 cgcagggcat cggcaatttg ctcggccgtc agatgctgcg gagcagacag aagaatcgtg
     2701 gcgatttgca gacgctgatc ggtgacccga attccgcagt cggcaagacg ggtcgcgact
     2761 ttcgcccagt tggattccgt tagggcagct tggctatcca tggcatttca ttatagcgcg
     2821 agcgatgaat ttagccgaaa gcacaggcgg cggcgacata aggatgagca aactgcgagg
     2881 ttatcccaac tttttcggcc gtgttcccga tcgagccagt ccccaggatc gtcgaactat
     2941 tgagcccgca gggccttggg tagtccgaag accacatgct cttcccgccc ggagagctcc
     3001 gagggcacct ggcctcccca gctgcgcagg cgttgtacca ccgcctggac ctgcacttcc
     3061 ggagcggagg ctccggcggt gacaccgacg ttgtccttgc catcgaccca cccgcgatcc
     3121 atgtcatccg gaccatcaac caggtagccg gggacacccg cgcgctcagc gagttcgcgc
     3181 agccggttcg aattggaact gctcttggag ccgacgacaa tcaacgcatc gcactgctcg
     3241 atcatctgtt tgaccgcatc ctgccggttc tgcgtcgcat agcagatgtc ctcgcggcgc
     3301 ggcgcctgca gtgacggaaa gcgggcgcgc agggtctcga cgatcgcagc cgtatcgtcc
     3361 acggacagtg tggtctgtgt cacgaacgca agattctgcg tatcgcgcac gcgcagcgtc
     3421 ttcacatcct cgatcccctg caccaggtac atgtgcccgc cgaaactggt atcgaagcgg
     3481 cccatcgtgc cttccacttc cgggtggccg gcatgaccga tcaggatgac ctcgcgtgct
     3541 tcgcgcgcga agcggctgac ctccatgtgt accttggtga ccagcggaca ggtcgcatcg
     3601 aagacctgca gcccacgacg caacgcctcc tcctccaccg cggtggagac gccgtgcgcg
     3661 gagaagatca ccgtagcgcc gtcggggacc tccgacagtt cctcgacaaa cacggcgccg
     3721 aggtcacgca gacgcgccac cacgtgatgg ttgtgcacga cctcgtgccg tacgtagatt
     3781 ggggtaccaa acatctgcag tgcgcgctcg acgatctcga tggcgcgatc gacgcccgca
     3841 cagaaacccc ggggattggc cagaatgatg cgcatggtgt gagtctacct gccttcgagc
     3901 cacgcatcga gcaacagcat cgcagcgccg atcgtgatgg cagaatcggc cacgttgaag
     3961 gccggaaagt agtgttcacc ccagtgagcg tgaacgaaat caatcacatg accgagccgc
     4021 aggcggtcga tcacgttgcc gagcgcgcca ccgaggatga gcgccaccgc actggccagc
     4081 agcttcgcgc cgcgatcgat ccgccgcagc cacagtacca gcaccacact gacgacgatc
     4141 gccagagcgg tgaatagcca gcgctgccag ccggcttcat ctgccagaaa gctgaatgcg
     4201 gcaccggtgt tgtgcaggcg cgtgatctcc agcaccggta acaccgggat ggactgatac
     4261 agacccagcc agcgctcgat cagcgttttg gtgagctgat cgagcgcgat gactgcgatg
     4321 gacagccaca gatagataag gccgcccgct ctcgcaccgg ccacgatcgt ggtctcgcct
     4381 ccaaaatcgg attgcgccat caggaatacc gccgtgcttc gccgggcagc gtgagattgc
     4441 cgacgcagcg caggcaaatc tgtgggtgct ccggcacctg acccacatcc ggccggtgat
     4501 gccagcagcg gatgcacttt gtgtgagtgc tggcgcgcac tcgaatccag acttcaccac
     4561 catcgacgct cggcgctgcg accgcatcga ccggcacggc cgcgcggtca ttcagcgcaa
     4621 cctcgtgcac cgttgcctcg gaggtgatca tcaggaagtg cagctcggca ccgaggcgcg
     4681 atagtcgccg atactgggga gcggtacacc acacctcgag ctcagcatcc agcggcgcac
     4741 cgatcgcccc gctgacacgc agccgctcca gttcgcgcgc cacatctgcg cgcagcgcga
     4801 tgagcgcggt ccagtcgatt tcatcatccg gtacttgagg caattcatgc caggtggtga
     4861 gaaacaccga ctgcggacgc tggccgcgca ggaaacccca catctcctcg gcggtaaagg
     4921 acagcatcgg tgccagccag cgcaccatgc tctcgccaat atgccacatc gccgtctgtg
     4981 ctgaacggcg cgcgtgactg ctggccggcg tcgtgtagag ccggtctttc aggatgtcca
     5041 gatagaagcc gcccagatcg accacgcaga agttgtgtac ctgctgatag atctgatgaa
     5101 actggtagtc ccggtaggat tgcacgacgt tgtcatgcag cgcgcgcgtg cgcgcaatcg
     5161 cccagcgatc aatcgctacc agctgcgaga tcgggaccga ctgctgcgga tcgaaatccg
     5221 atagattgcc cagcaggaag cgcaccgtgt tgcgcatccg gcgataagaa tcagtcagcc
     5281 gtcttagaat ttcctgcgac agactcatct caccggaata gtcggtcgca ccgatccaca
     5341 aacgcagaat gtccgcgccg agcgtgcgca tgatgtcctg cggctcgatg ccgttgccga
     5401 gcgacttgga catcttgcgg cccttgtcgt cgaccgtgaa gccgtgcgtc agcacgccgc
     5461 gatagggcgc gcgctcgtac agcgcttccg acatcagcag cgaactgtga aaccagccgc
     5521 gatgctgatc tgaaccctcc agatacagat ccaccggcgc gctgaagtcg ggacgaatcg
     5581 ccgcgacgca ttcgaatgac aggccggagt cggcccacac atccatcaca tcggtcagtt
     5641 tctcgtagtg ctcggcatcg gcgccgagca gctccttcgc atccagcgca aaccacgcat
     5701 cgatgccacc actttccact cgctgcgcga cctgtagcag cagttcctgc gtgcgcggat
     5761 gcagcgcggc ggtttgtttg tggacgaaca gcgccagcgg gacgccccag gttcgctggc
     5821 gtgagatgca ccagtcagga cgatgctcga tcatgctggt gatgcgctgc tcgccccagg
     5881 ccggtgtcca acgcacctgc ctgatgtcgc gcaacgcgtg ctcacgcagc ttgtgcgcat
     5941 ccatgctgat gaaccattgc ggcgtggcgc ggaaaatcag cggcgacttg tggcgccagc
     6001 agtgcgggta gctatgacgc agcggctcct gatgtagcag cgctgctttt tcgatgagct
     6061 gcgcaatgat cacggcttcc gccttgtcca cgcgcagtcc cgcgaccagc gctgtgtcgg
     6121 gcaggtattt cccatccgga ccgaccggat tggccaccgg taactggtat cgctgtgcga
     6181 ccagaaagtc ttcctgaccg tgcgccggcg cggtatgcac ggcgccggtt ccggcatcca
     6241 gtgtgacatg ctcaccgagg atcagcaggc tctggcgcgg caggaacgga tgcgagagtt
     6301 gcaggcgctc gagcacgcga ccgggaaatt ccgcgagctg ctcgatcgca gtcaacccgt
     6361 agcgcgccag gcaggagtcc ttcagttgcg aggccagcac cagcacttcg cgctgcgaat
     6421 tgcgcgtcac ggcgaccgcg acatagttga aatcaggtcc gaccgacacg gcctcgttgg
     6481 ccatcagggt ccagggggtc gtggtccaga tcacgaccga gaccggaccc ttgagggctt
     6541 cgcgcgcgac gccgatgcgc ttggcgaaat caccgcggtc cacgaccggg aaggcgacgt
     6601 cgatggcggt cgaggttcgg tcctcgtact cgacttccgc ctcggccagc gccgagcggc
     6661 agtccaggca ccagtgcacg ggctttgcac cgcgatacag atgtccgttg cggatgatct
     6721 tgcccagcgc acggatctgc tgcgcctcat agcgcggatc catcgtcaga taagggttat
     6781 cccaatcggc gaatacaccc agacgcttga agtcggcgcg ctgcgaatcg atctgctgat
     6841 tggcgaatgc acggcaggcg gcgcgaaatg ccgtggcatc cagtttgtgc ccgggtttgc
     6901 cgtgtttttt ctcgaccgcc agttcaatcg gcagaccatg gcagtcccag ccgggaatga
     6961 aagccgcgtc gcgcccgtcc atggtctgcg acttgacgac gatgtccttg agtattttgt
     7021 tgacggcgtg gcccagatgg atcacgccgt tcgcatacgg cggcccgtcg tgatagcgaa
     7081 atgctggccg tccacgcgaa gcctcacgaa tgcgcgcgta aatatcctca tcgagccagc
     7141 gtttgagctg cgcgggttca cgctgggcca gatcggcttt catcggaaag tcggtctgcg
     7201 gcagattgat ggtgtccttt ttgtagtcca ctaacgctca cctaattgac ggcgtgcctg
     7261 cgcggcgtcc cgatgcatct gctgcaccat cgcatccatc gatgcaaagc tcgcctcatc
     7321 gcgcaggcgg gcgacgaatt ccacttcgag ctcccgacca tacagatcgc cgctaaaatc
     7381 gaacagatgc acctccaata acatgtgcgt gccacccacc atcgggcgcg tgcccaggtt
     7441 cgcgacgccg ggccaggcgc taagaccggc tccatgcgcg cgtaccgcaa acacgccgga
     7501 tagcggcacc cgccgccgtt tgaccgcgac gttggcggtg gggaaaccga gcgtgcgtcc
     7561 gagacggtta ccgcagcgaa cccgtccgcg catgccgtag ggtcgaccca gaagccggcg
     7621 cgcatgcgcg aagtcaccga cgcgcaccgc atcgcgcacc tggccactgc tcacgcgcag
     7681 gccatcgatc ggcaccggcg aaatcacatc gacctcgaat ccgaactgcg agccacgctc
     7741 ggcgcaccat tgcgcgctgg cctccccgcc catgccgaac cggaagtcgt gaccgaccac
     7801 cacaatgcgc gcatgggcgg cctgtagcag ttgcatgaac tgcagacccg tcagcgtccg
     7861 cagtcgctcg ctgaaggtca gcacgcacaa gcgctcgagg cctgtgctcg agagcagccg
     7921 ccagcgctcg cgaaagttcg tgagccgcgg cggtgggtcc gcaggctgta gatactcgcg
     7981 cggcagcggc tcgaaactca ccagcatcgt tggcagcccg aggcgcgtgc cgtgcgcgca
     8041 cagccgctcg atcagtgcgt gatgccccag atgtatgcca tcgaaaccac cgatcgtgac
     8101 aacacaatca tgggcactat tggaatcgag tcggcggatg agctccatgg ttcttggcac
     8161 tggccgacct gccgctacag cagcgccgat cggcaggcag atgaggcgtc aaaagtccaa
     8221 acggcaaacg caaattatag ccataaccaa tgccctgccg cggccggttc ttgagggtgc
     8281 aggcccctga tttgacaagc tgaagcgtgc tcgccagact acgcggcccc gaaatggcgg
     8341 gtatgcagtc aacggctttc aggagttcgg cgtggcgaat acgaaatcgg cggaaaaagc
     8401 cgcccggcaa gctgccgagc accgcactca caatgtcacg ttgcgctcac gcatgcgcag
     8461 tgcgatccgc aaggtcaacg atgcggtcgc ctccggcaac aaggacgacg cccagaagag
     8521 ctatcaggcc gctgtgccgg tcatcgacag catggtcaat aagaagattc ttcatcgtaa
     8581 cgccgctgcc cgctataaga gccgcttggc cacccgcgtg aagagcgtcg gcgccacgcg
     8641 ctgagagcgc cgtcggcccg ctgacagcag accactagct gcggcctcgt tcgcggtctt
     8701 cgagcgccag cagtagcgac agtctgtcat ccccgcgcgc gacctcacgc cagtgccggc
     8761 cctcgggcca gggctcgagc gcacccgcca gcgcccacag cgtgttgagc gaggaagtca
     8821 cgcccgccag cttcaactga taaaacgctt ccaccgcgcc ccgtcgccgc aattcgtcgg
     8881 cgctatgaac cccgatttca ctcaacattt cctcggattt cgtgccgagg ttgcgcaggt
     8941 cgcggatgcg cagccgcagc ttgccggcgg caatgcgtgc acggaccttg gaattacgca
     9001 tcggatcccg ggtcggccgc gcctgcattg atccgcgtct ccagaaactg catgaccgct
     9061 tcggccagtt cccgcgtacc gcgcccggtg accgcggaga tcagaaaccg cggtccgcga
     9121 tagcgcagcc ggcgcgcgat gtccgccgcg cgcttctcgg cctcctcatc gggcagcaga
     9181 tcgcgtttgt tgactaccag ccagcgtggc ttggcggcaa gctcttggtc aaatttcttg
     9241 agctcaccga cgatcgcgcg cgcatcgcgc accggatcgg cgtccggatc cggcggcaga
     9301 atatcgatca ggtgcagcag cacgcgagtg cgctgcaggt gcttcagaaa acggatcccg
     9361 agacctgcac cgtcggcagc gccttcaatc agtcccggaa tgtcggccat cacaaagctg
     9421 cggtgttgac cacaattgac cacgccaagg ttcggataga gggtggtaaa gggatagtcg
     9481 gccaccttcg ggcgcgcagc cgaaacggcg cggatcagcg tggattttcc ggcgttcggc
     9541 agccccagca gcccgacatc cgcgatcact ttgagctcga gggccagcgt gcgcttctcg
     9601 ccgggcagcc cgggcccgaa ttgccggggc gctcgattgg tactcgactt gaagcgggta
     9661 ttgccccagc cgcccttgcc gccctgcgcg actttcagct cctggccagc cgtcgccagg
     9721 tccccgagcg cttcatcggt ctgcgcatcg cggacctggg taccgaccgg gaccgccaca
     9781 taaagatcct cgccactgcg gccggtgcag tcgttgcccg agccgccgtc acccgatgtc
     9841 gccttgaata cccgctcgat gcgaaagtcc gcgagcgtgt tgatgccttc cttggaacgc
     9901 agccacacgc tgccaccatc gccaccgtcg ccgccgtccg gaccgccaaa cggaacgaat
     9961 ttctcgcgcc ggaaactggc gctgccacgc ccaccattgc ccgcctgcac gcgcatggtg
    10021 gcttcatcga cgaacttcat cgggtgggcg cctgccgcag cgcggcatca ggcttggagc
    10081 tcttggggtg cccggacgac gggaaacggc cctcgccgga tcctcagttg ggcttgtcgc
    10141 tgttggcggc gggcgcctgc ggctgcacgc tcacgtacat gcgttgttcg gcgccgcgga
    10201 tacggaactc gacctgcccg gtgatctcgg caaataaagt gtgatcggtg ccggtcccga
    10261 cgttctcgcc ggcgcgcacc ttggtgccgc gctgacgcac caggatgtta ccggccagca
    10321 cgtgctggcc tccgtagcgc ttcacgccta agcgtttgga gtgtgactcg cggccgttac
    10381 gggtactgcc gcctgccttc ttgtgtgcca tggccttcgc tccgtcgccc tagggcgcgc
    10441 tgatgtccgt gacgcgcacc tgcgtgtaat gctggcggtg cgttttctgt cgcatatagt
    10501 gcttgcgacg gcggaatttg acgatcgtga ccttgtcgcc tttggcgtgc ttttcgatcg
    10561 tagcggtaac cttgccacct tgcagttgtg gcttgccgag gcgcacatca tcgccatcgg
    10621 cgatcagcag cacctcaccg aattcgacgg tcgaaccggg ctcgccctct agcttctcga
    10681 tgcgcagcac ggcacccttc tcgactctat actgtttgcc gcctgtggcg atgaccgcgt
    10741 acattgtctt caaactccgt gtgatcgtcg caccgctggt agcgagcgcc cggaacacaa
    10801 tatacagccg ggacactcgt tcgaagtgcg aaaaggtcgc gcattctaac gatgccgcgc
    10861 tggccggtca aacaacgatt tctcgtggag aaacatacag ttgaatagtg actacggcag
    10921 ccgcgagtcg ctcgagaaga ttcgcgcgct ggtgcgtacc gagctgacgg ccgtcgacga
    10981 ggccatccgc agcggcctgc acagcgcggt cggtctggtc gatcagatcg ccgatcacat
    11041 catcggtggg ggcggaaagc gcctgcgacc gctgctggtg gtgctggccg ggcgtgcctg
    11101 tgggggcact ggcacggagt gcgtacgggc tgccgctttt atcgaattca tccacaccgc
    11161 cacgctcctg cacgatgacg tcgtggacat gtcggctcgc cggcgcggcc gcgataccgc
    11221 gaatgaaatt ttcggcaacc aggccagcgt gctggtcggc gattttgtct actcgcgtgc
    11281 gttccagctg atggccgcga cgcgctcgca gcgggtcatc gagatcatgg ccgatgccac
    11341 caatctgatt gctgagggcg aggtccttca gctcatgaac gcacacgacc cggaaaccac
    11401 tgaggcgcgc tatctcgaag tcatccaccg caagaccgcg cagctgtttc aggctggcgc
    11461 cgaagtcgcc gcggtgatca gtggcgcccc cgagcgcgtg cagcgtgaac tgtccagcta
    11521 tgggaagcac ctgggcaccg cctaccagct cattgatgat gtgctcgact atcagtccga
    11581 ccccgagacc cgcggtaaga acttggggga tgatctggcc gagggcaagc ccacgctgcc
    11641 gctgatctat gcgctaagag aaggtgggcg ctgcagcgag ctgatgcgcg aagtcatccg
    11701 cgacggcggc cgcgagcggc tggaggacat cgtgggcgca attgagtccg cggggggcct
    11761 tgagtacact gcccggctcg cgcaatccga ggccgacgag gcgctgaccg cactgacggc
    11821 gctacccgat acaccctatc gggaagccct ggccgcactg gtgcgcttcg cggtcgcccg
    11881 caagcattga tgaaggctta attcggggtg tagctcagcc tggtagagca ctacgttcgg
    11941 gacgtaggag tcgcaggttc aaatcctgtc accccgacca agttctccgg gtccgttccg
    12001 accacatcgg taacgtttca ttccgggcac atgggtaaca cttttgcgcc gaaggggttc
    12061 tcggcgctgg ttatccgccc ggtctcgtga tcaaagaagc c
//
LOCUS       DATMRM010000184         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2588190, whole genome shotgun sequence.
ACCESSION   DATMRM010000184 DATMRM010000000
VERSION     DATMRM010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2588190"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VNZ06_05460"
     CDS             <1..810
                     /locus_tag="VNZ06_05460"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HWW20230.1"
                     /translation="PQLLMLSGIGPASELAAHGIEVKVDLPEVGRNLQDHLDFCTVQQ
                     STRAVTYEFNATQMMLVGLRYGLTHGGPGSSNLAEGGGFVRSALAIDQRPDVQFHFLP
                     AQLDDHGRHRLPGHGYTLHACALRPRSRGYVTLRSGSPEDSPRIFARYLSDPFDARVL
                     LEGIQLSRAVLSQAPFKPYRGKEVYPGEGVTAHDQIESVLRRKAETIYHPVGTCRMGA
                     DPASVVDPSLRVRGVEALRVVDASVMPMLIGGNTTAPTMMIAEKIAQTMLG"
     gene            837..1724
                     /locus_tag="VNZ06_05465"
     CDS             837..1724
                     /locus_tag="VNZ06_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWW20231.1"
                     /translation="MLEIRELSKTYANGVQALKSVSLQIPNGMFGLLGPNGAGKSTLM
                     RTIATLQDPDRGSIHLNGLDVLANKPAVRAQLGYLPQEFGVYPKVSAEAMLDHFAMLK
                     GVVVRSERKALVEALLQQVNLWGVRKRKLGTFSGGMRQRFGIAQALIGKPSLMIVDEP
                     TAGLDPEERNRFLNLLAEIGENVVVILSTHIVEDVTDLCPRMAIISEGRVLLSGQPAE
                     AMQALQGRVWRRSIPTASIEDYQRRFSVLSTRLVGGKTIVHVLADGKPEEGFEPVAPD
                     LEDVYFGQLRASGSSTLAA"
     gene            1740..>2320
                     /locus_tag="VNZ06_05470"
     CDS             1740..>2320
                     /locus_tag="VNZ06_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWW20232.1"
                     /translation="MFGEILRFELRQQLKSPLFWIVALAFGALAFGVAASDTIQIGGG
                     IGNTHRNAPYVVMQTLTVFSVLGMFLITIFVAGAALRDFEAGTAEMFFATPMPRTSYL
                     AGRFTAGYLTSLAVLIFVALGLWLGSLMPWLDPARLGPTPVAAYGYSFAIFVLPNLFF
                     VSALLFLLAILTRSLLGTYVGVIAFFVLWQIAGV"
BASE COUNT          366 a          741 c          765 g          448 t
ORIGIN      
        1 ccgcagctgc tgatgctctc gggcatcggg ccggcttcgg aactggcggc gcacggcatc
       61 gaggtgaagg tggatctgcc ggaagtgggc cgcaatctgc aggatcacct ggacttctgc
      121 actgtgcagc agagcacccg cgccgtcact tacgaattca acgccacgca gatgatgctg
      181 gtcggcctgc gctacgggct cacacacggc ggccccggct cgagcaacct cgccgaaggc
      241 ggcggcttcg tgcgctcggc gctcgcgatc gatcagcgcc cggatgtgca gtttcatttc
      301 ttgccggcac agctggatga tcatggccgc catcgactgc ccggacacgg ctatacgctg
      361 cacgcctgcg cgctgcggcc gcgcagccgc ggctatgtga cgctgcgctc gggtagccct
      421 gaggattcac cgcgcatctt tgcgcgctac ctgtccgatc cgtttgacgc cagggttctg
      481 ctcgagggca tccagctctc gcgggccgtg ctcagccagg cgcccttcaa gccctatcgt
      541 ggcaaggagg tctatccggg cgagggcgtg acggcgcacg atcagatcga atcggtgctc
      601 cggcgcaagg ctgaaacaat ttaccacccg gtgggtactt gtcgtatggg cgcggacccg
      661 gcatcggtgg tggacccctc gcttcgcgtg cgcggggtcg aggcgctgcg cgtggtcgat
      721 gcctcggtga tgccgatgct gatcggaggc aataccaccg caccgaccat gatgattgcg
      781 gagaaaatcg ctcagacgat gctcggctga cgacacaacg acaggggatc gctccaatgc
      841 tcgagattcg cgaactgtcc aagacctatg ccaatggcgt gcaggcgctc aagagcgtct
      901 cactgcagat cccgaacggc atgttcggtc tgctcggccc caatggtgcc ggcaagtcga
      961 cgctgatgcg cacgatcgcg acactgcagg atccggatcg gggcagcatt cacctcaacg
     1021 gcctggatgt gctggccaac aagcccgcgg tgcgcgccca gctcggctat ctgccgcagg
     1081 agttcggcgt gtatccgaag gtcagcgctg aggccatgct cgatcacttc gcgatgctca
     1141 agggcgtggt ggtgcgcagt gaacgcaagg cgctggtcga ggccctgctg cagcaggtga
     1201 acctttgggg cgtgcgcaaa cgcaagctcg gcaccttttc cggtggcatg cgccagcgct
     1261 tcggcattgc ccaggcgctg atcggtaagc cgagcctcat gatcgtggat gagcccaccg
     1321 cgggtctgga tccggaggag cgcaaccgat tcctcaatct gctggcggag atcggcgaga
     1381 acgtcgtggt cattctctcc acgcacatcg tggaggatgt caccgacctg tgtccgcgca
     1441 tggcgatcat ttcggaaggg cgagtgctgc tgagcgggca gccggccgag gccatgcagg
     1501 cactgcaggg gcgcgtctgg cgccggagca tcccgaccgc gagcattgaa gactaccagc
     1561 ggcgattctc ggtgctgtcc acgcgcctgg tcggcggcaa gacgatcgtt catgtgctgg
     1621 ccgatggcaa accggaggaa gggttcgagc cggtggcgcc ggatctggag gatgtgtatt
     1681 tcgggcagct gcgggcgtcg ggttcgtcga cgctggcggc ctgagcggcg ggaagagcca
     1741 tgtttggcga aatcttgcga ttcgagttgc gccagcagct gaagagtccg ctgttctgga
     1801 tcgtggcgtt ggccttcgga gccctcgcgt ttggcgtcgc cgcctccgac accatccaga
     1861 tcggcggtgg catcggcaat acccaccgca acgcgcctta cgtcgtcatg cagacgctga
     1921 ccgtcttcag cgtgctcggc atgttcctga tcacgatctt tgtcgccggc gccgcgctgc
     1981 gtgatttcga agcgggcacg gccgagatgt tcttcgccac gcccatgccg cgcacgagct
     2041 acctcgccgg gcgcttcacc gccggctacc tcacctcgct cgccgtgctg atcttcgtgg
     2101 cgctgggtct gtggctcgga tcgctcatgc cgtggctgga ccctgcacga ctgggtccca
     2161 cgcccgtcgc cgcctatggc tacagcttcg ccatcttcgt gctgcccaat ctgttcttcg
     2221 tctccgcact gctgttcctg ctcgcgatcc tgacgcgctc gctgctgggt acctacgtcg
     2281 gcgtgatcgc cttcttcgtg ctgtggcaga tcgcgggcgt
//
LOCUS       DATMRM010000185         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3198955, whole genome shotgun sequence.
ACCESSION   DATMRM010000185 DATMRM010000000
VERSION     DATMRM010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3198955"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..1616
                     /locus_tag="VNZ06_05475"
     CDS             207..1616
                     /locus_tag="VNZ06_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWW20233.1"
                     /translation="MRSDEHGFQHIIERERGLQRQLSAGRLSMIAIGGAIGTGLFLGS
                     GFAIGLAGPAVLVSYAIGAVIALLLMGCLAEMTIAHPTSGSFGAYAEYYVGPLAGFLV
                     RYAYWTCIVLAVGTEVTAVALYMHFWFPRAPAWLWIVAFSSLLIAINARSVNWFGTVE
                     YWFSATKVAAIVIFIVLASYLLWRAPAAPIHGAPAIGWRNYTAYGGFFPHGWWGAWVA
                     VIVAIFSYLSIEMIAVAAGEAQDPQLAVRRAFRSTVLRLILFYLVTLALVLAIVPWTR
                     AGLDESPFVKVMRALAVPGAAGIVNFVILVAALSAMNSQLYITTRMMFSLARAGYAPR
                     RFGVLSGHGVPAQALLLSSIGIACATLLSVTAPRSAYLLMVSISAFGAMFTWMMIFIT
                     HFFFRRARRAKPTVGFRMIGYPVTTLLGAALMAAVLLTTAFTEAFRLTLVFGVPFIGV
                     LCLLYRLRNARAASSARAQ"
     gene            1704..2162
                     /locus_tag="VNZ06_05480"
     CDS             1704..2162
                     /locus_tag="VNZ06_05480"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWW20234.1"
                     /translation="MAVKPVPDGYHTVTPYLIINGAAQAIDYYRNAFGASELLRMPDP
                     QGRIAHAELRIGDSVIMLADEHHEMGYRAPRTLGGTSVSILLYLVDVDTVFARAISAG
                     GKSLRPVADQFYGDRMGTLEDPFGHVWTIGTHIEDVSPEEMKRRMATAHA"
     gene            2276..>3285
                     /locus_tag="VNZ06_05485"
     CDS             2276..>3285
                     /locus_tag="VNZ06_05485"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HWW20235.1"
                     /translation="MDGRNGRRALGLPAQRVYGPSMVFNSLTFIVFFACVLALHSLPL
                     PWRVKKFNLLIASYLFYSAWNPPFVILLWVSTVVDWYAAQGLVKARRQAARHAWMLLS
                     VIANLGMLGYFKYGQFLLDSFSALLSSFGVVYQPAHYDIVLPVGISFYTFATMSYTLD
                     VYLRRAAPARNLLDYALFVTFFPHLVAGPIMRPTELVPQFAEPRRASAQQLRFGLALM
                     TIGLFNKVVLADTFLATAAERVYDGSRLPGALDSWAATLAFSGQIFCDFAGYSTTAIG
                     AALCLGFAMPDNFRFPYAAVGFSDFWRRWHITLSSWLRDYLYIPLGGNRHGQRRTYAA
                     LMT"
BASE COUNT          488 a         1073 c         1049 g          675 t
ORIGIN      
        1 agcgcggcgc gcagtccggc ggcggcgtcc ttagagctca gagcgtcgag gcttgacgct
       61 gcggcggccg tgccggcgat cacgagaagt ccgagcacca gtgactggcg gcagttgcgg
      121 ttcatgggat ccttcctgtt ggtcgagtct agacttcctt ctgcccaaag cctattagta
      181 cccgccgacg caccggatcc aagaagatgc gcagcgacga acacggcttc cagcacatca
      241 tcgaacgcga acgaggcctg cagcggcagt tgagtgccgg gcgcctgtcc atgatcgcca
      301 tcggcggggc gatcggcacg ggcctgtttc tcggcagcgg tttcgcgatc gggctcgcgg
      361 gcccagcagt gcttgtgagc tatgccatcg gcgctgtcat cgcgctgcta ctgatgggat
      421 gcctggcgga gatgaccatc gcacatccca ccagcggttc tttcggcgcc tacgccgaat
      481 actatgtggg cccactggcg ggcttcctgg tgcgctacgc gtactggacc tgcatcgtgc
      541 tcgcggtggg caccgaggtc accgcagtgg ctctctacat gcacttctgg tttccgcgcg
      601 cgccggcgtg gctgtggatt gtcgccttct cgagcctgct gattgccatc aatgcccgca
      661 gcgtgaattg gttcggtacg gttgagtatt ggttctcggc caccaaagta gcggcgatcg
      721 tgatattcat cgtgctcgcc tcgtatctcc tgtggcgcgc gccggcggca ccgatccacg
      781 gtgccccggc gatcggctgg cgcaactaca ccgcgtacgg tggctttttc ccccacggat
      841 ggtggggggc gtgggtggcg gtcatcgttg ccatattcag ctacctgagc atcgagatga
      901 tcgcggtcgc ggctggcgag gcgcaggatc cgcagctggc cgtgcgccgc gcgtttcgct
      961 ccaccgtgct gcgcctgatc ctgttctacc tggtgacgct cgcactggtg ctcgccatag
     1021 taccctggac gcgtgcgggg ctcgacgaaa gccccttcgt gaaggtcatg cgcgcgctcg
     1081 cggtgccggg ggccgcgggg atcgtcaact tcgtgatcct ggtggcggcg ctgtcggcca
     1141 tgaacagcca gctgtacatc acgacccgca tgatgttcag tctggcgcgt gccggctatg
     1201 caccgcgacg tttcggcgtg ctcagcggcc acggcgtgcc cgctcaggcg ctattgctgt
     1261 cgagcatcgg gatcgcgtgc gccacactac tgagcgtcac ggcaccgcgc tctgcttacc
     1321 tgctcatggt gtcgatttcc gcgttcggcg caatgttcac gtggatgatg attttcatca
     1381 cgcacttctt tttcagacgc gcgcgccgtg cgaagcccac ggtcgggttt cgcatgatcg
     1441 gctatcccgt cacgacgctg ctcggggcgg cgctgatggc tgcggtactg ctaaccacgg
     1501 cgtttaccga ggcgtttcgc ctcaccctgg tgttcggcgt gcccttcatc ggcgtgctgt
     1561 gcctgctcta ccgcttgcgt aacgctcgcg ctgcatccag tgcgcgcgct caatagctac
     1621 tggatggtgc ggcgatgcac aggagctgtt aacccgcacg ctgcggcgga gtaagctccc
     1681 cggatcactc aaggaggcgc gccatggccg tgaaaccggt tccggatgga taccacaccg
     1741 ttactcccta cctcatcatt aacggcgctg cgcaggcgat cgattattac cgcaacgcct
     1801 tcggcgccag cgaacttctg cgcatgcccg atccgcaggg caggatcgcg catgcggagc
     1861 tcaggatcgg cgattcggtc atcatgctcg cagacgagca tcacgagatg ggctatcgcg
     1921 caccgcgcac cctcgggggc acgagcgtca gcattctgct gtacctcgtc gacgtcgata
     1981 cggtgttcgc gcgcgcgatc agcgccggtg gtaagtcgct gcgcccggtt gccgaccagt
     2041 tctatggcga tcgcatgggc acgctcgagg acccgttcgg ccatgtctgg accatcggca
     2101 cccacattga agatgtgtct cccgaggaga tgaagcggcg catggccacc gcgcacgcct
     2161 gagtcagata ctcgccgctg tcgaccagga cgcgctcgcg tggcgtcgca gtcgcgcgcg
     2221 acggtgagga tcattgcgtt ggcggaacgc gccgatagac aatgacgcaa agcccgtgga
     2281 cgggcgaaac gggaggaggg ccctcggcct gccagcgcag cgggtttacg ggccgtccat
     2341 ggtattcaac tcgctcacgt tcatcgtgtt cttcgcgtgc gtgctcgccc tgcattcgct
     2401 gccgctgccg tggagagtga agaagttcaa cctcctgatc gccagctacc tgttctattc
     2461 cgcgtggaat ccccccttcg tgatcctgct gtgggtgtcg acggtcgtcg actggtacgc
     2521 agcgcagggg ctggtcaagg cgcgccgcca ggcggcgcgc cacgcctgga tgctgctctc
     2581 ggtgattgcc aatctcggca tgctcggcta cttcaagtac ggccagttcc tgctggacag
     2641 cttcagcgcg ctcctgagct cgttcggggt cgtctaccag cccgcgcatt acgacatcgt
     2701 gctgccggtc ggaatctcgt tctatacctt cgccaccatg tcgtacaccc tcgatgtgta
     2761 cctgcggcgc gcggctcccg cgcggaacct gctcgactat gcgctgtttg tgacgttctt
     2821 tccgcacctc gtcgccgggc cgatcatgcg acccaccgag ctcgtgccgc agttcgccga
     2881 gccgcgtcgg gcgagcgccc agcagctgcg cttcgggctg gcgctgatga cgatcgggct
     2941 gttcaacaag gtcgtgctgg ccgacacgtt cctcgccacc gcggccgagc gcgtgtacga
     3001 cgggtcgcgc ctgcccgggg cactggattc atgggcggcg acgctcgcgt tcagcggcca
     3061 gatcttctgc gacttcgcgg ggtattccac caccgcgatc ggggcggcgc tgtgtctggg
     3121 ctttgccatg ccggacaact tccgattccc gtacgcggcc gtcggctttt cggatttctg
     3181 gcgtcgctgg cacatcacgc tctcgtcctg gctgcgcgac tatctgtaca ttcccctcgg
     3241 tggcaatcgc cacggacagc gtcgcaccta cgcggcactc atgac
//
LOCUS       DATMRM010000186         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2276515, whole genome shotgun sequence.
ACCESSION   DATMRM010000186 DATMRM010000000
VERSION     DATMRM010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2276515"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VNZ06_05490"
     CDS             complement(<1..255)
                     /locus_tag="VNZ06_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003177920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWW20236.1"
                     /translation="MTREIARRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGAVKGR
                     KATRHATSDWMRLEQQRGISVTSSVMQFPFDECIVNLLDTP"
     gene            complement(273..761)
                     /gene="rimI"
                     /locus_tag="VNZ06_05495"
     CDS             complement(273..761)
                     /gene="rimI"
                     /locus_tag="VNZ06_05495"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HWW20237.1"
                     /translation="MSSVKREELFPTVEIRAMNDHDVPVIVEIERSGYQFPWSEGIFR
                     DCLRVGYICRVIDVGDVLAGYGIMSVGAGEAHILNVCVADEYRCRGLGRKLMLYLMQR
                     ARALGMEEAFLEVRPSNLIASRLYHALGFEQIGVRRGYYQAPAGREDASVLRRALAAI
                     PE"
     gene            complement(754..1416)
                     /locus_tag="VNZ06_05500"
     CDS             complement(754..1416)
                     /locus_tag="VNZ06_05500"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWW20238.1"
                     /translation="MNPQVRHAYLDALGLDSWALRQKAQPVRTPLPVPMAHEWSMLRE
                     RVAACTRCSLCATRTQTVFGVGDPAADWLIIGEAPGAEEDRLGEPFVGRAGQLLNSML
                     RAIGLSRTEVYIANILKCRPPGNRDPSATEAEQCLPYLSEQIALLRPKIILVVGRIAA
                     HNLLRTDLSLARLRQQVHYFGEARIPVVATYHPAYLLRELHEKRKAWEDLKFAREVRS
                     RV"
     gene            complement(1413..>2026)
                     /locus_tag="VNZ06_05505"
     CDS             complement(1413..>2026)
                     /locus_tag="VNZ06_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWW20239.1"
                     /translation="DGLDGRVARWTHTDSAFGKEYDSLSDMVSFGLAPAIVTYQWGVA
                     RISEYGPLWRRVGWLVCFFYAAAAALRLARFNSRASTQDKQYFEGLPSPSAAAIVAAF
                     VWAASAQTDIGLPGLVLAFVVTAIAGALMISRFAFHSFKQVNLSARVRFTHILLVPLA
                     FVLIASHPPAALLLLFGAYAASAPLQWAWRKMRRRSRPAPARP"
BASE COUNT          407 a          666 c          632 g          321 t
ORIGIN      
        1 gggcgtgtcg agcaggttga cgatgcactc atcgaagggg aactgcatga ccgaggaggt
       61 aaccgagatg ccgcgctgct gctccaggcg catccagtcg gaagtcgcgt ggcgcgtcgc
      121 cttgcggccc ttgacggcac ccgccatctg gatcgcgccg ccgaatagca gcagcttctc
      181 ggtcagcgtc gtcttacccg catcgggatg cgaaataatc gcaaatgtgc ggcggcgcgc
      241 aatttcgcgc gtcatatcgg ccgtcgtgct gctcactctg gaatcgccgc cagagcacgg
      301 cgcagcaccg acgcatcttc gcggcccgcg ggagcctggt aatagccgcg acgcaccccg
      361 atctgctcaa agcccagcgc atgatacagg cggctcgcga tcaggttcga ggggcgcacc
      421 tcgagaaacg cctcttccat ccccagcgcc cgcgcgcgct gcatcaggta cagcatcaac
      481 ttgcgcccca gtccgcggca cctgtattcg tcagcgacgc agacattgag gatatgcgct
      541 tcgcccgccc cgaccgacat gatgccgtag ccggcaagca catcaccgac atcgatcacg
      601 cggcatatgt atccaacccg cagacagtcg cgaaagattc cctcactcca cgggaactgg
      661 taaccggacc gctcaatctc gacgatcacc ggcacatcat ggtcattcat cgcgcggatt
      721 tcgactgtcg gaaaaagctc ttcgcgcttg acgctagaca cgacttcgca cctcacgggc
      781 aaacttgagg tcttcccacg ccttgcgctt ctcgtgcagc tcgcgcagca gatacgccgg
      841 gtgataggtg gcgaccaccg gaattcgtgc ctcgccaaaa taatgcacct gctgcctgag
      901 gcgggccagc gataagtccg tgcgcagcag attatgcgca gcgatgcgcc cgacgaccag
      961 gatgatcttc ggccgcagca gcgcaatctg ctcactgagg tatggcaggc actgctcagc
     1021 ctcggtcgca ctcggatcgc gatttccagg gggccggcac ttcaggatgt tggcaatata
     1081 aacttccgtg cgggacaggc cgatcgcgcg cagcatcgaa ttcagcagtt ggccggcacg
     1141 cccgacaaag ggctcgccca gccggtcctc ctctgcaccg ggcgcctcgc cgatgataag
     1201 ccagtcggcg gctggatcgc cgacgccaaa cacggtctgc gttcgcgtcg cgcacaagga
     1261 gcagcgggta cacgccgcga cacgctcgcg cagcatggac cactcgtgcg ccatcggtac
     1321 gggcaacggc gtgcgcaccg gctgggcttt ttgccgcagc gcccaggagt cgagccccag
     1381 tgcatcgaga tacgcatggc gcacttgcgg attcatgggc gtgcgggggc tgggcgggag
     1441 cgccggcgca ttttgcgcca ggcccattgc aggggcgccg acgccgcgta cgcgccgaat
     1501 agcagcagca acgcagcggg tgggtgggat gcaatcagca caaaagcgag cggcacgagc
     1561 aggatgtgcg tgaagcgcac acgggcgctt aagttgacct gcttgaagct gtggaatgca
     1621 aagcggctga tcatcaacgc gccggcgatc gccgtgacga cgaaggcgag cacgagtccc
     1681 ggcaggccaa tatcggtctg cgcgctcgcc gcccacacga atgcggcgac gatagcggcg
     1741 gccgacgggc tcggcaggcc ttcaaaatat tgcttatcct gggtactggc gcgtgagttg
     1801 aaacgcgcga ggcgcagcgc cgccgcagcg gcatagaaaa agcacaccag ccagccgacc
     1861 cggcgccaca gcggaccata ttcggaaatc cgcgcgactc cccactggta agtgacgatc
     1921 gccggcgcga gtccgaagga caccatgtcg gaaagactgt cgtactcctt gccaaaggcg
     1981 ctgtcggtat gggtccagcg cgccacccgc ccgtcgagac catcga
//
LOCUS       DATMRM010000187        10165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_94422, whole genome shotgun sequence.
ACCESSION   DATMRM010000187 DATMRM010000000
VERSION     DATMRM010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10165
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94422"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..546)
                     /gene="folB"
                     /locus_tag="VNZ06_05510"
     CDS             complement(169..546)
                     /gene="folB"
                     /locus_tag="VNZ06_05510"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HWW20240.1"
                     /translation="MSQHDSEDRIFLRGLTTQCIIGFIDWERLTPQTVVIDLEMPCDC
                     ARAARTDSVDDTVDYKRVAKRVLGWVGESKFQLVESLAHALALLLLAEFPFEWVRVSI
                     NKPGAISHSRDVGVSIERRRASS"
     gene            complement(577..1596)
                     /gene="tsaD"
                     /locus_tag="VNZ06_05515"
     CDS             complement(577..1596)
                     /gene="tsaD"
                     /locus_tag="VNZ06_05515"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HWW20241.1"
                     /translation="MQILAIETSCDESAVAVLDEERGLRSHELWSQIDLHRIFGGVVP
                     ELASRDHVRRLLPLVRSALDRAEVDAKQIQGVAYTAGPGLVGALLTGAALARSLSFGW
                     GVPAIGIHHLEGHLLAPMLETPPPPFPQLALLVSGGHTMLIDVRGMGDYRLLGQTRDD
                     AAGEAFDKTAKLLGLPYPGGPQLAQLAERGRPGLYQFPRPMLDREGFEFSFSGLKTAV
                     MQAVRSETLSDQGRADVAHAVQDAIVSTLINKSMRALEFTGYRALVVAGGVGANHSLR
                     TRLTQLTASVGARVFFPRLEFCTDNAAMIAVAGLYRLRAGEQAGSAIEVRARWPLDEL
                     RPPGT"
     gene            1703..1912
                     /gene="rpsU"
                     /locus_tag="VNZ06_05520"
     CDS             1703..1912
                     /gene="rpsU"
                     /locus_tag="VNZ06_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HWW20242.1"
                     /translation="MPSVRVRENEYFDAALRRFKRACEKAGVLAELRRREFYEKPTQE
                     RKRKKAAAVKRHMKRVSRDFVRPAR"
     gene            1913..2356
                     /locus_tag="VNZ06_05525"
     CDS             1913..2356
                     /locus_tag="VNZ06_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWW20243.1"
                     /translation="MSLKDRIQEDMKAAMRSGEKERLGTIRLILASIKQREVDERITL
                     DDAQVISVLEKMGKQRRESITQFQAGGRADLVDKENAELKVISAYMPSQLSAAELDVL
                     IAEAIAQSGAASVKDMGKVMNIIKSKAQGRADMAAVGARIKARLS"
     gene            2389..4194
                     /gene="dnaG"
                     /locus_tag="VNZ06_05530"
     CDS             2389..4194
                     /gene="dnaG"
                     /locus_tag="VNZ06_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HWW20244.1"
                     /translation="MAGRIPQAFIDELIARADLVELIGARIPLKKAGREYKACCPFHN
                     EKTPSFCVIPEKQFYHCFGCGAHGTALGFLMEYDRLPFPEAVEDLATRVGLTVPHELG
                     SGGGAETGGDSTAPLYEILTKVSDFYHSALLRNERAQAYAGQRGLTPETVERFRIGYA
                     ADGWSEVLRRFGDNERAQTQLLASGLIIERERASGSERYYDRFRDRLMFPIRDTRGRV
                     IAFGGRVLDQGEPKYLNSPETALFHKGKELYGLYETRLARRALKRLLVVEGYMDTVRL
                     HQAGLTYAVATLGTATTAEHLKRAFRLVSEIVFCFDGDRAGRSAAWRALQQVLSEARE
                     GREIRFLFLPEGEDPDSLVGREGRERFEARLDSALPLSEYLVSQLLEQADVSHADGKA
                     HFVALARPLIDKVAPGVYRELLLERVAAAINLPAARLELWMRSPESQGKAPARDERVS
                     PRSTHSAPRLTGRGNLVTQSISLLLHFPAAANAVTDAQREELERLTQPGVHVLRELLE
                     QQRVSAAVTTAQTLERWRERAEYRRFCELAANDPLVPDRAAAGAELKEAVARLLGLES
                     RRRLEVLIDKARAETLDASEKQELQTLTGALSKDS"
     gene            4602..6581
                     /gene="rpoD"
                     /locus_tag="VNZ06_05535"
     CDS             4602..6581
                     /gene="rpoD"
                     /locus_tag="VNZ06_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HWW20245.1"
                     /translation="MAVTPPAKTLVVPVLSKDSGAATHKRIPGAPAAHGEALPRRGNG
                     LPEDRQSQLKLLIARGKEQGYLTYAQVNDHLPAEIVDPEQIEGIVNTINDMGIPVYEK
                     APDSESLLASEPSSPADDEAIEEAAAALAALDAELGRTTDPVRMYMREMGTVELLTRE
                     GEIRIAKRIEEGLDAVRVALAKYPFTHEHLIKTYELIKAGQARLIDLVVGFIDPNAPD
                     VIAQPQNPTKLELEADSEDKDDDAEDGDEDEEEAIDTGPDPEEVARRMSSLQKVHLQA
                     LASMAKLGTEDPKTQKLRKKLAGEFMELKLSPRMFDALTDQLRAHVNEVRQIEKEIMV
                     IAVRDSGMPRKDFISTFPKNETSARWLQKHMKAGKKYSAPLTRLKDEIERRQKKLMDL
                     EVKYHLAIHDIKDINREVSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLI
                     QEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKL
                     NRISRQMLQEMGREPTPEELAVRMEMPEDKVRKVLKIAKEPISMETPIGDDEDSHLGD
                     FIEDQSVASPIDQATMESLRETTHSVLAQLTPREAKVLRMRFGIDMNTDHTLEEVGKQ
                     FDVTRERIRQIEAKALRKLRHPSRSEQLRSFLMDD"
     gene            complement(6591..7025)
                     /locus_tag="VNZ06_05540"
     CDS             complement(6591..7025)
                     /locus_tag="VNZ06_05540"
                     /inference="COORDINATES: protein motif:HMM:NF022428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2802 domain-containing protein"
                     /protein_id="HWW20246.1"
                     /translation="MTMTWFQLSWLHEISQSALAAAVVTLGLVILLELRSIKLLRRSI
                     DNSLGRVFEQLDLLRFEQQPVATDAVAAPAARNATPVLSNVGPAATATAPAAGNPYVA
                     AAALASTGMKAEEIAQRCGLAAGEARLLASLAAARIGRRADA"
     gene            complement(7139..7990)
                     /gene="motD"
                     /locus_tag="VNZ06_05545"
     CDS             complement(7139..7990)
                     /gene="motD"
                     /locus_tag="VNZ06_05545"
                     /inference="COORDINATES: protein motif:HMM:NF006541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotD"
                     /protein_id="HWW20247.1"
                     /translation="MPRRKKYEEHDNHEAWAIPYGDLVTLLLAFFVVMYAMSSVNEGK
                     YRVLSDSLAEAFHGQAHSAQPLEDPGPHAQPIEQMPLTQVNRMIMGQLPSRHLMQAPQ
                     QTDPAAATTEGDQNGAEAAASLTDASANELGHVAEDVDAAMASLIEAGQVHVHRHDNW
                     VVVDISSDILFGSGQARLSAAAMGVLQRLADSLRTFPNAIRVTGHTDNRPIKTVAFPS
                     NWELSSARAASVVHLFMDRGITAQRLSVMGFGEYRPVMSNNTETGRNANRRVEVMIMG
                     RDANPEP"
     gene            complement(8016..8756)
                     /locus_tag="VNZ06_05550"
     CDS             complement(8016..8756)
                     /locus_tag="VNZ06_05550"
                     /inference="COORDINATES: protein motif:HMM:NF006583.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HWW20248.1"
                     /translation="MDILSIVGLLLAMVAILLGAILKGAGLHALVSAAAFMIVVVGTI
                     AAICVQTPMEVMRRAMLMFPWIIRPPNHEPRKLIKKLLEWSHIARRQGLLGLETMIDY
                     EPDPFLRKGLQLVVDGSEPDTIRSTLEFDMHAREAADTRAAKVFEGMGVYSPTLGIIG
                     AVLGLMAVMQNLDDPSKLGHGIAAAFTATVYGIGMANLFFLPVANKLKVAVQARTHLD
                     EMQLEGLIGIAQGENPRIIEGKLLSFLH"
     gene            complement(8764..9498)
                     /locus_tag="VNZ06_05555"
     CDS             complement(8764..9498)
                     /locus_tag="VNZ06_05555"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HWW20249.1"
                     /translation="MTGGVRAYSDVQPAQDMDALVRKHADLVKRIAYHLAGRLPPQVE
                     VDDLIQAGMIGLLEAAQNFATGRGASFETFAGIRIRGAMLDALRRLDWAPRSVHRKSR
                     AAAVALREVEAKRGGEASETDVAAHMGVSVNEYQRIIQDALGCQLLRLNDADDGEESA
                     LDRLADESPNPESSALGDSLRQAIIDAIQNLPERERLVLSLYYEQELNLKEIGVVLKV
                     TESRVCQLHGQALLRLKAQLSDWHSP"
     gene            complement(9495..>10165)
                     /locus_tag="VNZ06_05560"
     CDS             complement(9495..>10165)
                     /locus_tag="VNZ06_05560"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HWW20250.1"
                     /translation="TPRCTLEHVLNGERRLEEVILQTASGVRVVPASSGVAKMAALAI
                     TEQAAIVQAFSALPGALDVLVVDTAAGIHDSVLQFCQAAQQVLVVLRDEPTSLTDCYA
                     LIKVLSRSHGVRHFRVIANMTRAAGEGESVFRRLQRVADRYLEVVLEYAGEIPDDRSV
                     KQSVQQQRPVLEAFPNCPAARAFKRLAVAAARWPMPLGPSGRLEFFLERMLTRTSPRL
                     KVLK"
BASE COUNT         2037 a         3239 c         3124 g         1765 t
ORIGIN      
        1 cgaacccaaa cgcgggattt cgatagcagc gcgaaaagcg cacgtccggg aagcgcatct
       61 tcagcgcacg cgccgcctgt tgcatgcgcg tggacggttc aatattgctt cccagggcga
      121 tgaacacctg gggccccgag acctggctca ttgggcaagc actgccgctc agctgctcgc
      181 ccggcgtcgt tcgatgctga ctcccacatc acgcgaatga ctgatggccc ccggcttgtt
      241 gatactgacg cgcacccact cgaacggaaa ttccgccaac agcaacagcg ccagagcatg
      301 ggccagcgac tcaaccagct gaaacttcga ctcgcccacc caacccagaa cccgcttggc
      361 aactcgcttg tagtccacgg tatcgtcaac gctatccgtg cgcgccgcac gcgcgcagtc
      421 gcaaggcatc tccagatcga tcaccacggt ctgcggcgtc agccgctccc agtcgatgaa
      481 gccgatgatg cactgggtgg tcagccctcg cagaaagata cggtcttcgc tgtcgtgttg
      541 gctcatgcgg gcgaagtcta gcctgtcagc tcaggtttaa gtgccgggcg ggcgcagctc
      601 atcgagcggc cagcgtgcgc gcacctcgat ggcgctgccg gcctgctcac cggcacgcag
      661 ccgatataat ccggcgaccg ctatcattgc agcgttgtcg gtacagaatt ccagtcgcgg
      721 gaagaacacg cgcgccccaa cgcttgcggt cagctgtgtg agccgggttc gcagcgagtg
      781 gttggcgccc acgccgccgg ccactaccag cgcgcgatag ccggtgaact ccagtgcccg
      841 catgctcttg ttgatgagcg tactgacgat cgcatcctga accgcatgcg cgacatcggc
      901 ccgcccctga tctgacaggg tttccgagcg cactgcctgc atcaccgctg tcttcagtcc
      961 cgaaaagctg aactcgaagc cctcccgatc gagcatgggc cgcggaaact gatagagacc
     1021 ggggcgaccg cgttcggcga gctgcgccag ttgtggacct ccggggtaag gcagtccgag
     1081 cagcttcgcg gtcttgtcga acgcctcgcc ggccgcgtca tcgcgcgtct gccccagcag
     1141 ccggtagtca cccatgccgc gcacgtcgat cagcatcgtg tgcccgcccg agaccagcag
     1201 tgcgagctgt ggaaacggtg gcggcggcgt ctcgagcatc ggcgccagta gatgcccttc
     1261 gagatgatga atcccgatcg ccggaacgcc ccagccgaaa ctcaaggagc gtgccagcgc
     1321 agccccggtc aacagcgcgc cgaccaaccc tggacctgcc gtgtaggcga cgccctggat
     1381 ttgcttcgca tccacctcag cgcgatccag ggcgctgcgc accaggggca acaggcggcg
     1441 cacgtgatcg cgcgaggcaa gctctggcac gaccccaccg aagattcgat gcagatcaat
     1501 ctggctccag agctcgtgcg aacgcagtcc tcgctcctca tcgaggaccg ccaccgcgct
     1561 ttcgtcgcag gaagtctcaa tcgccaggat ttgcatgtga tcgttgcatt ttgcgttttg
     1621 tcgcaggtgt cattagaatc gcggcccttg ctctgtgggt ttcgttcaaa aagcgctttc
     1681 gaatcaagag gttaaaattt cgatgccgag tgttcgcgta cgtgagaacg aatattttga
     1741 cgccgccctg cggcgattca aacgggcctg tgaaaaggca ggtgttctcg ccgaactgcg
     1801 acggcgcgaa ttctatgaaa agccgaccca ggaacggaaa cgcaagaagg cagccgccgt
     1861 caagcggcat atgaagcgcg tctcgcgcga cttcgttcgc cccgcccgct agatgagcct
     1921 gaaggatcgc atccaggaag acatgaaagc ggcgatgcgt tccggagaaa aggagcgcct
     1981 cggcacgatc cgcctgattc tggcgtcgat caagcagcgg gaagtcgatg agcgcatcac
     2041 gcttgacgat gcccaggtga tctcggtcct cgagaagatg ggcaaacagc ggcgcgaatc
     2101 gatcacccag tttcaggccg gcggacgcgc cgacctggtg gacaaggaaa atgccgaact
     2161 gaaggtcatc agcgcctata tgccgtcgca gctatccgca gcggaactgg acgtgctgat
     2221 tgccgaagcc attgcgcaga gcggtgccgc gtcagtgaaa gacatgggca aggtcatgaa
     2281 tatcatcaag tccaaggccc agggacgtgc cgacatggcc gccgttggcg cgcgcatcaa
     2341 agcccgtctg tcctaggagc cggtctgagt tctagactac cggctcgtat ggccggccga
     2401 attccacaag cctttatcga tgagctgatc gcccgtgccg atctggtgga actcatcggc
     2461 gcgcgcatac cgctgaagaa agcgggccgc gagtacaagg cgtgctgccc gttccacaac
     2521 gagaaaaccc catcgttctg cgtgatcccg gagaagcagt tctaccactg cttcggctgc
     2581 ggcgcgcacg gcaccgccct cggctttctg atggagtacg accggctacc gtttccggaa
     2641 gcggtcgagg acctggccac gcgagtgggc ctaacggtac cgcacgagct cggtagtggc
     2701 ggcggcgctg aaaccggtgg tgattccacc gcgcctctct atgagattct gaccaaggta
     2761 tccgacttct atcactcggc gctgctgcgc aacgagcgcg cacaggccta cgccggccag
     2821 cgcggtctga cacctgaaac cgtcgaacgg tttcgcatcg gctacgccgc cgatggttgg
     2881 agcgaggtgc tgcggcgttt cggcgacaac gagcgggcgc agacccagtt gctggcgagc
     2941 gggctcatca tcgagcgcga gcgagccagc ggcagtgagc gctattacga tcggttccgc
     3001 gaccgactga tgtttccgat acgcgatacg cgcggtcgcg tgatcgcctt tggtggccgg
     3061 gtactcgacc agggcgagcc aaagtacctg aattcgcctg agaccgcgct gtttcacaaa
     3121 ggcaaggagc tgtacggtct gtacgaaacg cgcctcgcgc gccgtgcgct caaacgcctg
     3181 ctggtggtgg aaggctacat ggacacggtc cgcctgcacc aggccggact cacctatgcc
     3241 gtcgcgacac ttggcaccgc gaccaccgcc gagcatctga agcgagcgtt tcggctggtg
     3301 agcgagatcg tcttttgctt cgacggcgat cgcgctggcc gctcagccgc gtggcgtgcc
     3361 ttgcagcagg tgctgtccga agcacgcgag ggacgtgaga tccgatttct atttttgccc
     3421 gaaggcgaag atccggattc gctggtcggg cgcgagggcc gcgagcgctt cgaagcccgg
     3481 ctcgattcgg ccctgccgct gtccgaatat ctcgtttcgc agctgctcga gcaagcggac
     3541 gtgagccacg cggacggcaa agcgcatttc gtcgcgcttg cacgtcccct gatcgacaaa
     3601 gtcgcccccg gtgtctaccg cgagctgtta ctggagcgcg tcgccgccgc gatcaacctg
     3661 ccggctgcgc gtctggaact gtggatgcgc tcaccggaga gtcaaggcaa agccccggcg
     3721 cgcgacgagc gagtcagtcc gcgcagcacg cattccgccc cgcgcctcac cgggcgggga
     3781 aatctcgtca cgcaatcgat cagcctgctg ctgcactttc cggcagcggc caatgccgtg
     3841 accgacgccc agcgggagga actggagcga ctcacccagc ccggtgtcca tgtgctacgc
     3901 gaactgctcg aacagcagcg ggtcagtgcg gcggtgacca ccgcgcagac gctcgaacgc
     3961 tggcgcgaga gagccgaata tcgccgcttc tgcgaactgg ccgccaatga cccgctggtg
     4021 cctgatcggg ctgcggccgg tgccgagctg aaggaagcgg tggcgcgact gctcgggctc
     4081 gagtcccggc ggcggctgga ggtgctgatc gacaaggctc gcgccgagac gctcgatgct
     4141 tccgaaaagc aggaacttca gacgctcacg ggtgccttat caaaggactc ctagaggcgt
     4201 cgcgctgatg cttaagcccc gcccgatcac gtatcgagcg agcacttaag gggaccggtc
     4261 accataatca acccacggtt cggctaccgt ttctttctgc gtgggacttg aatactggcg
     4321 ccggtgcagc aattctgggt attatcgcca gctaacccca tgaaggaccg accgatcaca
     4381 gcctttcgtc gtcaagcgac ggaacgggct gcttcgtcac attcacttca agagacacca
     4441 tgacacgcaa gccgtcaaag ccagcaccgt tagcccgcaa agccgctccg cgcgctgccg
     4501 ctgcggcctc tacgcctgcc cgcaagggcg ctcccgctcc ggcgggggcg gcccggcgcc
     4561 ccgttgctgc cgcgaccagg tcggccgacc cgcataagaa ggtggctgtc acaccgcccg
     4621 cgaagacctt ggtggttccg gtcctatcga aggacagtgg tgctgcgacg cacaagagaa
     4681 tccccggtgc acctgccgcg cacggcgagg ccctaccccg gcgtggcaat ggattgccgg
     4741 aggaccggca atcgcaactg aaactgctga tcgcccgcgg caaagagcag ggctatctca
     4801 cgtatgcgca ggtgaacgat cacctgcccg ccgagatcgt cgatcccgag cagatcgaag
     4861 gcatcgtcaa cacgatcaat gacatgggaa ttccggtgta cgagaaggca ccggacagcg
     4921 aatcgctgct cgccagcgag cccagttcac cggccgatga cgaggcgatc gaggaggcgg
     4981 cggccgccct ggcggcactc gatgccgaac tcggccgtac caccgatccg gtgcgcatgt
     5041 acatgcgcga gatggggacg gtggaactgc tgacgcgcga aggcgaaatc cgcatcgcca
     5101 agcgcatcga ggaaggtctc gacgccgtgc gcgtcgcgtt ggcgaagtat ccgttcacgc
     5161 acgagcacct gatcaagacc tatgaactca tcaaggccgg ccaggcccgt ctgatcgatc
     5221 tggtagtcgg attcatcgat ccgaacgcgc cggacgtgat cgctcagccg cagaacccga
     5281 ccaagctgga gctcgaggcc gatagcgagg acaaggacga cgacgccgaa gacggtgacg
     5341 aggacgaaga ggaagccatc gataccggtc cggatccgga ggaagtggcg cgccgcatga
     5401 gttccctgca aaaggtacat ctgcaggcac tcgcctccat ggcgaaactc ggcaccgaag
     5461 acccgaagac acagaagctg cgcaagaagc tggcgggcga attcatggag ctgaagctct
     5521 cgccgcgcat gttcgatgcg ctgaccgatc agctgcgtgc gcacgtcaac gaagtgcgcc
     5581 agatcgagaa ggagatcatg gtgatcgcgg tgcgtgactc aggcatgccg cgcaaggact
     5641 tcatctcgac attcccgaag aacgagacca gcgcgcgctg gctgcagaag cacatgaagg
     5701 cggggaagaa gtattccgcg ccgctgacgc gcctgaagga cgagatcgag cgccgccaga
     5761 agaaactcat ggacctcgag gtgaagtacc acctcgccat tcacgacatc aaggacatca
     5821 accgcgaagt gtcgatcggc gaagccaagg ccaggcgcgc caagaaggaa atggtcgagg
     5881 ccaacctgcg tctggtgatt tcgatcgcga agaagtacac caaccgcggc ctgcagttcc
     5941 tggacctgat acaggaaggc aacattggcc tgatgaaggc ggtcgacaag ttcgaatacc
     6001 gccgcggcta caagttctcg acctatgcca cctggtggat acgccaggcc atcactcgtt
     6061 cgatcgccga tcaggcgcgc acgatccgca ttccggtaca catgatcgag accatcaaca
     6121 agctgaaccg catttcgcgg cagatgctgc aggagatggg ccgcgaaccg acacctgaag
     6181 agctggctgt ccgcatggaa atgccggaag acaaggttcg caaagtcctc aagatcgcca
     6241 aggaaccgat ctcgatggaa acgccgatcg gcgatgacga agactcacac ctcggggatt
     6301 tcatcgagga tcagtccgtt gcctctccca tcgaccaggc cacgatggaa tcgctgcgcg
     6361 agaccacgca ctcggtgctg gctcaactca cgccgcgcga agccaaggtg ctgcggatgc
     6421 gtttcggtat cgacatgaat accgaccata cgctcgagga agtgggcaag cagtttgatg
     6481 tcacgcgcga gcgtattcgt cagatcgaag ccaaagcact gcgcaagctg cgtcatccga
     6541 gccgcagcga gcagctgcgc agcttcttga tggatgactg accccgcgac tcaagcatcc
     6601 gcgcgccggc caatacgagc cgctgccaac gaggcgagta gtcgggcttc gccagcggcc
     6661 aggccgcagc gttgcgcgat ctcttcggct ttcatcccgg tcgaagccag cgctgcggct
     6721 gccacataag gattgcccgc cgcgggcgca gtggcggttg ccgctgggcc gacgtttgac
     6781 agcaccggcg tcgcattacg ggcggccggg gccgctaccg catcggttgc taccggctgt
     6841 tgctcgaaac gcagcaggtc cagctgctcg aacacgcgac ccagactgtt atcgatcgaa
     6901 cggcgcagca gcttgatcga gcgcagctcg agcagaatga ccaatcctaa agtcaccacg
     6961 gcagccgcca gggcggactg tgaaatctcg tgcagccagc tcagttggaa ccaggtcatt
     7021 gtcattgact tcgactcctg taacgcctcg agcgtcatca ttccgacagc tgcggctcaa
     7081 acgcagccct tgtcggatat aggagcagga agcgtgccaa ccgcacgccg acataagctt
     7141 acggctcggg gttggcgtcg cgccccatga tcatcacctc gactcgacgg ttggcgttgc
     7201 gaccggtttc ggtgttgttg gacatcaccg gccggtactc accgaacccc atcaccgaca
     7261 gacgttgcgc agtgatgccg cggtccatga acagatgcac cacactcgcg gcgcgcgcgg
     7321 atgacagttc ccagttggag ggaaaagcaa cggtcttgat cggacggttg tcagtatgac
     7381 cggtgacgcg gatggcattg ggaaacgtgc gcagtgagtc ggccagccgc tgcagcacgc
     7441 ccatcgccgc tgccgacaat ctggcttgcc cactgccgaa cagaatgtcg ctgctgatat
     7501 ccaccacgac ccagttgtcg tgccggtgga catgcacctg accggcctcg atcagcgatg
     7561 ccatcgccgc gtccacgtct tccgcgacgt gtccaagttc attggcactc gcgtcggtca
     7621 gcgaagccgc cgcctcggcg ccgttctggt caccctcggt agtcgcggcc gcgggatccg
     7681 tctgttgcgg cgcctgcatc agatgacgcg aaggtaattg gcccatgatc atgcggttca
     7741 cctgcgtgag cggcatctgc tcgatcggct gagcgtgcgg tcctggatcc tcgagcggct
     7801 gcgcggagtg cgcctgaccg tggaacgctt cggcgagcga gtcggacaac actcgatatt
     7861 tgccttcgtt gaccgacgac atcgcgtaca tgacgacgaa aaatgccagc agcagcgtca
     7921 ccaggtcgcc atacggaatc gcccaggcct cgtggttgtc gtgctcctcg tacttcttac
     7981 gccgcggcat ggtgcgtctc cgtgggcata ctcatctagt gcaggaagct gagtagcttg
     8041 ccttcgatga tgcgcgggtt ttcaccctgg gcgatgccaa tcaggccttc gagctgcatt
     8101 tcatccagat gggtacgcgc ctgcaccgcg accttgagtt tgttggcaac cggcagaaag
     8161 aacaggttcg ccatcccgat accgtagacg gtagcggtga aggccgctgc aataccgtgg
     8221 cccagctttg acggatcatc cagattctgc atgacggcca tcagtccaag taccgcgccg
     8281 atgattccaa gggttggtga gtagacgccc atgccctcga acaccttcgc ggcgcgcgta
     8341 tcggccgctt cgcgcgcgtg catatcgaac tccagggtgc tgcggatcgt gtccggctca
     8401 ctgccgtcca ctaccagctg cagccctttg cgcaggaacg gatcgggctc gtagtcgatc
     8461 atggtttcca gcccgagcag cccctgacgg cgcgcaatgt ggctccattc gagcagtttc
     8521 ttgatgagct ttcgcggctc atggttggga gggcgaatga tccatggaaa catcagcatc
     8581 gcccggcgca tcacctccat cggtgtctgc acgcagatgg cggcgatggt cccgaccacg
     8641 acgatcatga atgcggccgc ggacaccagc gcatgcagcc cggcaccctt gaggatcgcg
     8701 ccgagcagga tcgcgaccat cgcaagcaac agaccgacga tgcttaagat gtccacgccg
     8761 gccctacggc gagtgccaat ccgagagctg cgccttcaga cgcaacaacg cctgcccatg
     8821 caactgacag actcgtgatt cggtgacctt gagcaccacg ccaatttcct tgaggttcag
     8881 ttcctgttcg tagtagagcg acagcactaa acgttcccgc tcgggcagat tctggatagc
     8941 gtcgatgatg gcctgtcgca gtgagtcgcc gagtgcactc gactccggat tcggcgactc
     9001 atcggccagc cgatcgagcg cggattcctc gccgtcgtcg gcatcgttca ggcgcagcag
     9061 ctggcagccc agcgcgtcct gaatgatgcg ttgatattca ttgaccgaga cacccatatg
     9121 ggcggcgacg tcggtttcgc tggcttcgcc acctcgcttg gcctccacct cgcgcagtgc
     9181 caccgcagcg gctctggatt tgcggtgtac cgagcgtggt gcccagtcca gccgacgcaa
     9241 tgcatcgagc atcgctccac ggatgcggat gccggcaaag gtctcaaagc tggcgccacg
     9301 gccggtcgcg aaattctgtg cggcctccag cagaccgatc attccggcct ggatcagatc
     9361 gtccacctcg acctgcggcg gcagccgccc ggcgagatgg taggcgatac gcttcaccag
     9421 atcggcgtgc ttgcgtacca gcgcgtccat atcctgcgct ggctgcacgt ccgaataggc
     9481 gcgtacgccg cctgtcattt gagaaccttt agacgcggcg aagtgcgcgt cagcatgcgc
     9541 tccaggaaaa attcaagacg gccgctgggg ccgagcggca tcggccagcg tgcggcggcc
     9601 accgccaagc gcttgaaggc tcgtgctgcc ggacagttgg ggaatgcttc cagcaccggg
     9661 cgctgctgct gtaccgattg cttgaccgac cggtcgtcgg ggatttcccc ggcgtactcg
     9721 agcaccactt cgaggtagcg atcggcaacg cgctgcagcc gtcggaacac gctttcaccc
     9781 tcacccgcag cacgcgtcat gttggcgatc acacggaagt gcctcacacc atgactgcgg
     9841 ctgaggactt tgatgagcgc gtaacaatcg gtcagcgagg tcggctcatc acgcaatacc
     9901 accagtacct gctgtgcggc ctgacagaac tgcaatactg agtcgtgaat gccggcggcg
     9961 gtgtccacca ccagcacatc gagcgcgccg ggcaacgcgc tgaatgcctg aacgatcgcc
    10021 gcctgctcgg taatggccag agccgccatc ttcgcgacgc cagaggatgc cggtacgaca
    10081 cgcacaccgg acgcggtctg cagaatgacc tcttcgagcc ggcgctcgcc attcaatacg
    10141 tgctcgagcg tgcagcgcgg agtca
//
LOCUS       DATMRM010000188         6872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2895626, whole genome shotgun sequence.
ACCESSION   DATMRM010000188 DATMRM010000000
VERSION     DATMRM010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2895626"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VNZ06_05565"
     CDS             <1..528
                     /locus_tag="VNZ06_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWW20251.1"
                     /translation="AVGNEPFFVHLADDLIDADVPALKQMAQTYASRGGSVLGVEEVP
                     RKDTDKYGIVETEAGDGTVSRIKGIVEKPKPDVAQSTLAVVGRYILTPGVFQELERVG
                     QGAGGEIQLTDGIARLLQREPVFAHRFTGRRYDCGNKLGYLQATVELSLRHPVLGKDF
                     RSYLKRIAGTLDDKT"
     gene            690..1577
                     /gene="motA"
                     /locus_tag="VNZ06_05570"
     CDS             690..1577
                     /gene="motA"
                     /locus_tag="VNZ06_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HWW20252.1"
                     /translation="MLAIIGSVVVLVSVVGSFVVQGGSVLLLWHPSEIITIVGAALGA
                     FLISNPMKVVKKAFSASIGLVRGPKYKREDYVDLLKLIYDILVKIRKSGMLAIEADIE
                     HPEKSKLFTEYPRILADHHMVEFITDCLRLIIGGNLDPHELESLLDYELETHHQEAGE
                     PAHAVQKVADALPGFGIVAAVLGIVNTMASIEGSSSAAIGEKVGAALVGTFLGILVAY
                     GFVGPISSALEHRMHDEGKAFDVVKMALVASVRGYAPPVAVEFARKLLWSDVRPSFSD
                     LENHLKGKKGGAAKEGAAA"
     gene            1574..2713
                     /gene="motB"
                     /locus_tag="VNZ06_05575"
     CDS             1574..2713
                     /gene="motB"
                     /locus_tag="VNZ06_05575"
                     /inference="COORDINATES: protein motif:HMM:NF006548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HWW20253.1"
                     /translation="MSDKESERPILIVKKIKKAAGHHGGAWKVAYADFVTAMMAFFMV
                     MWLIASVNKETRAAIFDYFKNPSMDQGKSPKAAPGQMGPGGASTSVINLRGGLDARKS
                     SSPISTGLGTPSNGSVTDRPNMKLAAAEEAKKAAAAEERKKLESLLTQLNQAIGTSQA
                     LAPFKNQLLLDITPEGLRVQIVDAQNRPMFDLGSSKLKNYTNDIMRALAPYLNSVPNH
                     ISLAGHTDTTPYVVAGGYNNWDLSTDRANAARRALESGGLKSDKISRVVGLSSSVLFD
                     KENTRNPINRRISIIVMTQEAEESALKTDTATEIPASAAAQALHRDGYPSDGAAPPAA
                     GSTPPPADKSGALPVIEVPNAPTPSAVTVKPAPSAAATAAPAPGR"
     gene            2773..4155
                     /locus_tag="VNZ06_05580"
     CDS             2773..4155
                     /locus_tag="VNZ06_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HWW20254.1"
                     /translation="MAREGSVLSTIGNTPLLELRNLDVGCCRLFVKLENQNPTGSIKD
                     RIGLSMIEAAERDGRIRPGGDPPVTIIEATAGNTGLGLALVASQKGYRLLLVIPDKMA
                     QEKILHLRALGAQIRLTRSDVNKGHPEHYQDLAARLARETANSFYVNQFENPANPLAH
                     EATTGPEIWAQMGQMVDAVVAGVGSGGTLTGLSRYFARVAPYVEMVLADPKGSVLTDV
                     VKTGRIQQPAGSWLVEGIGEDFVPPNCDLSRVLHAYAIADAESFYTARALLEKEGILG
                     GSSTGTLVAAALRYCREQKSPKRVVTLVPDSGNKYLGKMYNDFWMFDQGLLERPRHGN
                     LLDLIVRRYQEGGAVTLAPADTLLLAYRRMRLFDVSQVPVMQGEQVIGIVDESDLVMH
                     LHTDPSRFQDRVDSAMVRTVETIAPNAPIADLLPIFQRDHVAIVAERGRFVGLITRID
                     LLNYLRKQLG"
     gene            4162..5349
                     /locus_tag="VNZ06_05585"
     CDS             4162..5349
                     /locus_tag="VNZ06_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWW20255.1"
                     /translation="MKHDKRSARAAFATRTIHGGQSPDPSTGAVMPPIYATSTYAQSS
                     PGVHQGFEYSRSQNPTRFAYERCVADLEHGTRAFAFASGLAATATFLELLKPGDHVVA
                     SDDMYGGSRRLFTRVRESSAGLKFTYVDLRDARQVEAAIRPETRLLWVETPSNPMLKL
                     ADLAALSAIARRHRMLAAADNTFATPYLQRPLETGFDVVMHSGTKYLSGHSDVVNGVL
                     VVGDNPELSERLSFLQNSVGAVAGPFDSFLALRGLKTLALRMQRHCENALELASFLER
                     HPNIQRVHYPGLASHPQHALAQEQMRDPRGHSAYGGMISIELKGGLEQSKRFLERCEI
                     FALAESLGGVESLIEHPAIMTHASVPREARLALGITDTLCRLSVGIEEVADLKADLKQ
                     ALG"
     gene            5355..>6872
                     /locus_tag="VNZ06_05590"
     CDS             5355..>6872
                     /locus_tag="VNZ06_05590"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HWW20256.1"
                     /translation="MQLKRLLLLIVCLGARVALAADPQPYSVKFESTGNKALDGALKA
                     SSQLDSLRTSAPAGPFALIGRAQSDVERLQTVCESFGYYRREITITIAGHPLDQVDLP
                     GELLALPKSPTVVVQIMVQLGPLFHVRKVTLDGDVSDNARAAFALESGAPAVAGQVLA
                     AGQRLQDALQDEGHAFARVDTPIAFEDAHDPVLDLTFKVSAGPIYRIGDISIQGLKRV
                     HTRFAQDRVSIHRGELYSPSRIERARSDLLGLGVFSGVTVQLPKQEEVSGESLPVIFA
                     VNERKRHAVSFTAGYSTDLGGSGGATWTDRNAFGNAEVLQVTSNLVDLGGSATKSPGY
                     QVLATLSKPDFLRSDQSLQYSVGLLKQNLQAYDQDAITAGVAINRTLSSRWKASVGLT
                     LEHEEIIQQEIVPVIDPTSHMQATNPKTGQPETAPADIVNHYTLLSIPITLKYDTTGL
                     SNPLDDPRHGLRLSGSAAPTESFNSTANNSATGGDRTPHATFLVVQGQFSTYLDLN"
BASE COUNT         1310 a         2182 c         2203 g         1177 t
ORIGIN      
        1 gcagtgggca acgagccgtt cttcgtccat ctcgcggatg atctgatcga cgccgatgtg
       61 cccgcgctca agcagatggc gcagacctat gcgtcgcgcg gcggcagcgt gctcggtgtc
      121 gaggaagtgc cgaggaagga caccgacaaa tacgggatcg tcgagaccga ggccggggat
      181 gggaccgtca gtcgcatcaa gggcatcgtc gagaaaccca agcccgacgt cgcgcagtcc
      241 accctggcgg tggtcggccg atacattctg acgccgggtg tgttccagga actggagcgt
      301 gtcgggcagg gcgcgggcgg ggagatccag ctcaccgacg gcatcgcgcg gctgttgcag
      361 cgcgagccgg tgttcgccca ccgcttcacc ggacgacgct acgactgtgg caacaagctc
      421 gggtatctgc aggccacggt cgagctctcg ttgcggcatc ccgtactagg caaggacttc
      481 cgcagctatc tgaaacgaat cgcgggcacg cttgacgaca aaacgtagtg accggcgtca
      541 cagttcgcgc ccttgaattc gtgcgctcag gggcaaaaaa tggctttcag atcagcgatg
      601 taaccggcta gccgagcttt caagaaacct ctcgggcggc cgatatccct agcgggttcg
      661 atttagcagt accggtagtt ccatcgctca tgcttgccat catcggctcg gtcgtcgttt
      721 tggtgtccgt cgtcggcagc ttcgttgtcc aaggcggcag cgtcctgctg ctctggcatc
      781 cgagcgaaat catcacgatc gtcggcgccg cgctgggcgc gttcctgatc agcaacccga
      841 tgaaggtcgt caagaaggcg ttctcggcct cgatcggact ggtcaggggt ccgaagtaca
      901 agcgcgagga ctacgtcgat ctgctgaagc tgatctacga catcctggtc aagatccgca
      961 agtccggcat gctggcgatc gaggccgaca tcgagcatcc ggagaagagc aaactcttca
     1021 ccgaataccc gcgcattctg gccgaccatc acatggtcga gttcatcacc gactgtctgc
     1081 gactgatcat cggcggcaac ctcgacccgc acgaactgga aagcctgctc gactacgagc
     1141 tcgaaaccca tcatcaggaa gccggcgagc cggcgcatgc ggtacagaaa gtcgccgacg
     1201 cgctacccgg cttcggcatc gtggccgcgg tgctcggtat cgtgaacacg atggcctcga
     1261 tcgagggctc cagctccgcg gccatcggcg agaaggtcgg cgcggcgctg gtcggtacct
     1321 tcctcggcat tctggtggcg tacggcttcg tgggtccgat ctcctcggcg ctcgagcatc
     1381 gcatgcacga cgaaggcaag gcgttcgacg tggtgaagat ggcgctggtg gcgagcgtgc
     1441 gcggctacgc gccgccggtc gcggtcgaat tcgcacgcaa gctgctgtgg tcggatgtgc
     1501 gtccgagctt ctcggatctt gagaatcacc tcaagggcaa gaagggcggc gcggcgaaag
     1561 aaggagcggc cgcttgagcg acaaggaaag cgaacgcccg atcctgatcg tcaagaagat
     1621 caagaaggcc gccggtcatc atggcggcgc gtggaaagtc gcctacgccg acttcgtcac
     1681 cgcgatgatg gccttcttca tggtcatgtg gctgatcgcg tcggtcaaca aggaaacccg
     1741 cgcggcgatc ttcgactact tcaagaatcc cagcatggac cagggcaagt cgccgaaagc
     1801 ggcgcccggc cagatgggtc ccggcggcgc cagcaccagc gtgatcaatc tgcgcggcgg
     1861 tctggacgcg cgcaaatcct cctcaccgat ctccaccggc ctgggcacac cgagcaacgg
     1921 ctcggtcacc gatcgcccga acatgaagct ggctgcggcc gaagaagcca agaaggccgc
     1981 ggccgccgaa gagcgcaaga aactcgaatc gctcctgacg cagctcaacc aggcgatcgg
     2041 cacgagtcag gcgctggcac cattcaagaa tcagctgctg ctcgacatca cgccggaggg
     2101 actgcgtgtg cagatcgtgg acgcgcagaa ccgtccgatg ttcgatctgg gcagctcgaa
     2161 gctcaagaac tacaccaacg acatcatgcg ggcgctggcc ccgtacctga acagcgtgcc
     2221 gaaccacatc agtctggccg gccacaccga caccacgccg tacgtggtcg ccggcggcta
     2281 caacaactgg gatctgtcca ccgaccgcgc gaatgccgcg cgccgtgcgc tggagagcgg
     2341 cggattgaaa tccgacaaga tctcgcgcgt ggtcggcctg tcctcatcgg tgctgttcga
     2401 caaggagaac acccgcaatc cgatcaaccg gcgcatcagc atcatcgtga tgacgcagga
     2461 ggcggaggag tcggcgctga agaccgatac cgcgacggaa attcctgcca gtgccgccgc
     2521 gcaggcattg catcgcgatg gctatccgag cgacggcgcc gcgccccccg cagcaggcag
     2581 cacgccgccg ccggccgaca agtccggcgc actgccggtg attgaggttc ccaacgcgcc
     2641 gacgcccagc gccgtcaccg tcaaaccagc gccctcggcg gcggccactg ccgcacccgc
     2701 acccgggcgc tgaacgcgaa agcgtccggc ccgtgatagt ctgcgccgcc tgatccatag
     2761 gtcggaggac gtatggcacg cgaaggttcg gtgctgagca cgatcggcaa tacgccgctg
     2821 ctggaactgc gcaatctgga cgtcggctgc tgccggctgt tcgtcaaact ggaaaaccag
     2881 aatcccaccg gctcgatcaa ggaccgcatc ggcctatcaa tgatcgaggc ggccgagcgt
     2941 gacggccgta ttcgcccggg tggtgatccg ccggtgacga tcatcgaggc caccgcaggc
     3001 aacaccggac tgggactggc actggttgcc tcgcagaagg gctaccgcct gctgctggtg
     3061 attcccgaca agatggcaca ggagaagatt ctgcatctgc gcgcgctggg tgcgcagatc
     3121 cgcctgacgc gcagcgacgt caacaagggg caccccgaac actatcagga tctggcggcg
     3181 cgactggcgc gcgagaccgc gaatagtttt tacgtcaatc agttcgaaaa tcccgccaat
     3241 cctctggcgc acgaggccac taccggtcct gagatctggg cgcagatggg tcagatggtc
     3301 gatgcggtcg tggccggcgt cggcagcggc ggcacactga cgggtctgtc gcgctatttc
     3361 gcgcgcgtcg ccccctacgt ggaaatggtg ctggccgatc cgaagggctc ggtgctcacc
     3421 gacgtcgtca agaccgggag aattcagcag cccgcgggct catggctggt cgagggtatc
     3481 ggtgaggatt tcgtgccgcc gaactgcgat ctgtcgcgcg tgcttcatgc ttacgcgatc
     3541 gcggatgcgg agagcttcta caccgcccgc gccctgctgg agaaggaagg catcctcggc
     3601 ggatcgagca ccggcacgct ggtggccgcc gcgctgcgct attgccgcga acaaaaatcc
     3661 ccgaaacggg tcgtaacact ggtacccgac agcggcaaca agtatctggg caagatgtac
     3721 aacgacttct ggatgttcga tcagggcctg ctcgagcgcc cgcggcacgg caatctgctc
     3781 gatctgatcg tgcgtcgcta tcaggagggt ggagcggtca cgctcgcgcc ggccgacacc
     3841 ctgctgctgg cataccggcg catgcgtctg ttcgatgtgt cgcaggtgcc ggtgatgcag
     3901 ggcgagcagg tgatcggcat cgtcgatgaa tcggatctgg tcatgcatct gcataccgat
     3961 ccgtcgcgct tccaggatcg cgtggacagc gcgatggtgc gcaccgtcga gaccatcgca
     4021 ccgaacgcgc cgatcgccga tctgctgccg atctttcagc gcgatcacgt ggcgatcgtg
     4081 gccgagcgcg ggcgcttcgt cggtctgatc acgcgcatcg atctgttgaa ctacctgcgc
     4141 aagcagctgg gctagagcct catgaagcac gacaagcgtt cagcgcgagc ggccttcgca
     4201 acccgcacga tccacggcgg acagtcgcct gatccgagca ccggcgcggt gatgccgccg
     4261 atctatgcga cctcgaccta cgcgcaaagc agtcccggcg tgcatcaggg cttcgagtac
     4321 tcgcgtagtc agaatccgac gcgttttgcg tacgagcgct gcgtcgccga cctggaacat
     4381 ggcacgcgcg cgttcgcgtt cgcctcagga ctggcggcga ccgccacctt cctcgaactg
     4441 ctcaagccgg gcgatcacgt cgtggcctcc gatgatatgt acggcggcag ccggcggctg
     4501 ttcacgcggg tgcgtgagtc ctccgcgggg ctgaagttca cctatgtgga cctgcgcgat
     4561 gcgcgtcagg tcgaagcggc gatccggccg gaaacgcgcc tgctgtgggt ggagacgccc
     4621 agcaacccga tgctgaagct ggccgatctg gccgcgctca gtgccattgc ccgccgtcac
     4681 cggatgctgg cggcggctga caacactttt gcgactcctt acctgcagcg cccgctggaa
     4741 acaggatttg atgtcgtgat gcactcgggc accaaatact tgagcggcca ctccgatgtg
     4801 gtcaatggcg tgctggtggt cggcgacaac ccagagctgt ccgaacgtct gagctttctg
     4861 cagaactcgg tcggtgccgt ggccggcccg ttcgactcgt tcctcgcgct gcgtggtctg
     4921 aagacgctgg cgctgcgcat gcagcgccac tgcgagaatg cactcgagct ggcgtcgttt
     4981 ctcgagcgtc acccgaatat ccagcgtgtg cactacccgg gactggcctc gcatccgcag
     5041 cacgcgctgg cgcaggaaca gatgcgcgat ccgcgcggac acagcgccta cggcggcatg
     5101 atctcgatcg aactgaaagg cggactggag cagtccaagc gcttcctcga acgctgcgag
     5161 atttttgctc tggccgaatc actcggcgga gtggagagcc tgatcgaaca tcctgccatc
     5221 atgacccacg cgagcgtgcc gcgtgaggca cgcctggcgc tcgggatcac tgatacgttg
     5281 tgccgtctgt cagtcggaat cgaagaggtt gcggatctca aggctgattt gaagcaggcg
     5341 ctcggctagg agcaatgcag ctcaagcgcc tcctactgct catagtctgc ctgggtgcac
     5401 gggtcgcgct ggcggccgat ccgcagccgt acagcgtcaa atttgagtcc accggcaaca
     5461 aggcgctcga tggcgcgctc aaggccagct cgcagctcga ttcactgcgc accagcgcgc
     5521 cggccggacc gttcgcgctg atcggtcgcg cacagtctga cgtcgagcgc ctacagacgg
     5581 tatgtgagag tttcggctac taccggcgcg agatcacgat cacgatcgcg ggacatccgc
     5641 tcgaccaggt cgatctgccc ggcgagctgc tggcgttgcc gaagtccccc acggtcgtgg
     5701 tgcagatcat ggtgcagctc gggccgctgt ttcacgtgcg caaggtcacg ctcgatggcg
     5761 atgtcagcga caacgcgcgt gccgcgtttg cactggagag cggtgcgccg gcggtcgcag
     5821 gccaggtgct ggcggccgga cagcgcctgc aggatgcgct gcaggatgag ggtcacgcct
     5881 tcgcccgggt cgatacgcca atcgcgttcg aggatgcgca cgatccggtg ctggacctga
     5941 ctttcaaagt gagcgcaggc ccgatctacc gcattggcga catcagcatt cagggattga
     6001 agcgcgtgca cacccgcttt gcgcaggatc gtgtgagcat ccatcgcggc gagctctaca
     6061 gcccgagcag gatcgaacgt gcgcgctcgg atctgctggg gctaggcgtg ttctccggcg
     6121 tcacggtgca gctgccgaag caggaggagg tcagcggcga gagcctgccg gtgatctttg
     6181 cagtcaatga gcgcaaacgg cacgcggtca gcttcacggc cggctactcg accgatctcg
     6241 gcggcagcgg tggcgcgacc tggaccgatc gcaacgcctt cggcaatgcc gaagtgctgc
     6301 aggtcacctc caacctcgtg gatctgggcg gcagcgccac caagtcgccc ggctatcagg
     6361 tgctggcgac gctcagcaaa ccggatttcc tgcgcagcga tcagtccctg cagtacagcg
     6421 tcggcctgct gaagcagaat ctgcaagcct acgatcagga tgccatcacc gcaggcgtcg
     6481 cgatcaatcg cacactgtcg tcgcgctgga aggccagtgt cggcctgacg ctcgagcacg
     6541 aagagatcat tcagcaggag atcgtgccgg tgattgatcc gactagccac atgcaggcga
     6601 ccaatccgaa gaccgggcag cccgagacgg caccggccga catcgtcaat cactacacac
     6661 tgctttcgat cccgatcacg ctgaagtacg acaccacggg attgtccaat cccctggatg
     6721 atccgcggca cggcctgcgt ctatccggat cggctgcgcc aaccgaatcg ttcaacagca
     6781 ctgcgaacaa ttccgcgacg ggaggcgatc gcactccgca cgcgacgttc ctggtggtac
     6841 agggccagtt ctccacctac ctggatctga at
//
LOCUS       DATMRM010000189         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1354779, whole genome shotgun sequence.
ACCESSION   DATMRM010000189 DATMRM010000000
VERSION     DATMRM010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354779"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VNZ06_05595"
     CDS             <1..184
                     /locus_tag="VNZ06_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20257.1"
                     /translation="ATLDGAIAKFGAAAAEAVQQTMRELQEMRREQASSLVIPDSAGG
                     GLPGGGLIGGGSRSRR"
     gene            complement(193..1995)
                     /locus_tag="VNZ06_05600"
     CDS             complement(193..1995)
                     /locus_tag="VNZ06_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005044939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWW20258.1"
                     /translation="MPIARLDQVSLSFGLRPLLDHADLLIRKGERVCLLGRNGEGKSS
                     LLKLLTREIAPDSGEVWVRPGARVAVLEQEVAADSAHTVREVVASGVAPGEEHEGTWQ
                     NDLQVDQVMARLQLNADAVVSALSGGWRRRAMLGRALVSAPDLLLLDEPTNHLDIEAI
                     TWLEEMMSGFRGALLFISHDRAFVRRLAMRIVELDRGQLRVWRGSYDDYVVQKSAALE
                     AEATQAALFDKKLAQEEVWIRKGIEARRTRNEGRVRALKALRVERSLRRDRIGQMEVR
                     VQDAVMSGKLVFEAIGVTHAFDGEPLIRNFSTRIMRGDRIGIIGPNGCGKSTLIRLLV
                     GELTPTAGVIRRGTGLQPAYFDQQRDQLDYSASIMDNVTGGSGDTVTIDGRARHVAGY
                     LRDFLFPPERLRAPVSMLSGGERNRLLLARLFAKPSNLLIMDEPTNDLDAESLELLEE
                     LVADYTGTLLLVSHDRAFLDNVVTGTLVFEGEGRLEEYVGGYSDWLRQRKNPPPGKGA
                     VPAAAAPAPLATATPPKVRRMSYKDQRELSMLPAKIEALESEQRRLQGQIADPEWFRA
                     DTGRATEGLQRLQTLDAELAAAYERWDLLESLRP"
     gene            complement(2046..>2245)
                     /locus_tag="VNZ06_05605"
     CDS             complement(2046..>2245)
                     /locus_tag="VNZ06_05605"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HWW20259.1"
                     /translation="VEYDCQKNRVRPNLAIYYTDNNLRGVMQSIEMDPKQTSWGPIVP
                     GTLDETNFQWACSPDHRARAH"
BASE COUNT          386 a          769 c          692 g          398 t
ORIGIN      
        1 cgctacgctc gatggggcga ttgcgaaatt tggcgcagct gccgccgaag cggtgcaaca
       61 gacgatgcgc gagctgcagg aaatgcgccg tgagcaagcc tcatcgctgg tgattccgga
      121 cagcgccggc ggcggactcc ccggaggcgg cctgatcggc ggcgggagcc gttcgcgccg
      181 ctaggctagg cactagggcc gcagcgattc gagcaagtcc cagcgctcgt aagcggcggc
      241 aagttcggcg tccagcgtct gcaggcgctg caaaccttca gtcgcgcgtc ccgtatccgc
      301 gcggaaccac tcggggtccg cgatctgtcc ctgcagtcgc ctctgctcgg actccagcgc
      361 ttcgattttg gctggcagca tcgagagttc gcgctggtcc ttataggaca tgcgtcgcac
      421 cttggggggc gtggccgtgg cgagcggtgc cggcgcggca gcggctggca ccgcgccttt
      481 gccgggcggc gggttcttac gctgcctgag ccagtcacta tacccaccga cgtattcctc
      541 gagacggcct tcaccctcga agaccagcgt gccggtcacg acgttgtcga gaaaggcccg
      601 atcatggctg acgagcagca gcgttccggt gtagtccgcg accagttctt caagcagttc
      661 caggctttcc gcatccagat cattcgtcgg ctcgtccatg atcagcaggt tgctgggttt
      721 ggcgaacaat cgcgccagca gcagtcgatt gcgctcacca ccggataaca tgctgacagg
      781 cgcacgcaag cgttccggcg gaaacaggaa atcccgcaga tatcccgcta cgtggcgcgc
      841 ccgcccgtcg atcgtcacgg tatcgccact gccaccggtg acgttgtcca tgatcgacgc
      901 gctgtaatcg agctgatcac gctgctgatc gaagtacgcc ggctgcagcc cggtaccgcg
      961 acggatcact ccggcggtcg gtgtcagttc accgaccagc aggcggatca gcgtgctctt
     1021 gccacagcca ttcggcccga tgatgccgat tcgatcgcca cgcatgatgc gggtggagaa
     1081 attgcggatc agcggctcac cgtcaaaggc gtgcgtcacg ccgatggcct cgaataccag
     1141 cttgccggac atcaccgcgt cctggacccg cacctccatc tggccaatgc gatcacgccg
     1201 caggctgcgc tcgacgcgca gcgccttcag cgcccgcacc ctgccctcgt tgcgcgtgcg
     1261 gcgcgcctcg atccccttgc ggatccagac ctcctcctgc gcgagtttct tgtcgaacag
     1321 cgccgcctgc gtcgcctccg cctcaagcgc cgccgatttt tgcacgacgt agtcatcgta
     1381 gctgccgcgc cacacccgaa gctgcccgcg atcgagctcg acgatgcgca tcgcgagacg
     1441 gcgcacaaac gcacgatcgt gactgatgaa cagcagcgcg ccgcggaatc cgctcatcat
     1501 ctcttcgagc caggtgatcg cctcgatatc gaggtgattt gtcggctcat cgagcagcag
     1561 caggtccggc gccgacacca gcgctcgccc cagcatcgcg cgccgccgcc agccgcctga
     1621 cagcgcgctc accacagcat ctgcgttgag ctgcagtctc gccatgacct ggtcgacctg
     1681 cagatcattc tgccaggtac cctcgtgttc ctcgccgggg gccacgccgc tcgcgacgac
     1741 ctcacgcacg gtatgggcac tgtcggccgc aacctcctgc tcgagcaccg cgacgcgagc
     1801 gcccggacgc acccacacct caccgctatc cggcgcgatc tcgcgcgtca ggagtttcag
     1861 cagcgatgac ttgccctcgc cgttgcggcc cagcaggcag acccgctcac ccttgcggat
     1921 caaaagatcc gcatgatcga gcagcggccg caggccgaaa cttaaggaaa cctggtcgag
     1981 gcgtgcgatg ggcattgcgg cgccgagtat acgggggcca ccgatcgacg gcgcaggcgt
     2041 ggcagtcagt gcgccctggc tcggtggtcc gggctgcagg cccactgaaa attggtttcg
     2101 tcgagcgtgc ccggcacgat cgggccccac gaggtctgct tgggatccat ctcgatggac
     2161 tgcatgaccc cgcgaagatt attgtccgtg taataaatcg ccaggttcgg gcgaacccgg
     2221 tttttctggc agtcgtattc gactt
//
LOCUS       DATMRM010000190         3331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2896695, whole genome shotgun sequence.
ACCESSION   DATMRM010000190 DATMRM010000000
VERSION     DATMRM010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2896695"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VNZ06_05610"
     CDS             <1..267
                     /locus_tag="VNZ06_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWW20260.1"
                     /translation="AEALNQQDLATQAAADTLSVSRTRYALGSISALELLVVQRNYQQ
                     ARIAQVQAQAARYADTAALFQALGGGWWNRPPSKATPTAAAGPN"
     gene            341..1516
                     /locus_tag="VNZ06_05615"
     CDS             341..1516
                     /locus_tag="VNZ06_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWW20261.1"
                     /translation="MAVIALVLVIGGVKGFGVYKMMRGFAAQEPQKFTVSTAKAEYRD
                     WQPKLTAVGSVRAERGTDLSAEVAGIVEAIQFESGDDVKAGQLLLQLRSADEVAHLES
                     LKANAVLAETTYHRDEAQFAAQAISQATLDTDAANLRGTKAQVAEQQALVDKKFVRAP
                     FAGHLGVRAVDLGQYLSPGTKIVTLQQLDLIYVDFFLPQQDLSQVNVGQKVQASSDTY
                     SGLRFAGEILAIDPLVDVNTRNVQIRAALKNPGHKLLPGMSASVDIEVGGPQRYITLP
                     QTAISYNPYGATVFIIVPQSAVASKDGGKEKSGGETSSAAQQSPPFKSDGHDLAAKQV
                     FVTTGATRGDQVAILKGVTEGDQVVTSGQIKLKNGALVTINNDVQPSSDPNPAPSEQ"
     gene            1525..>3331
                     /locus_tag="VNZ06_05620"
     CDS             1525..>3331
                     /locus_tag="VNZ06_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW20262.1"
                     /translation="MNVTDLFIRRPVLATVVSLLILLLGIRALGALPIRQFPKTENAT
                     VTVTTQYYGASADLIAGFITTPIEAAVAEAQGIDYLTSQSSNGVSTVTATLRLNYDSN
                     RALAEINTKVNSVLNQLPTGTQQPVISVQVGETVAGMYLGFNSDTLPPNQITDYVIRI
                     VQPKLQTIAGVQNAELLGGRQYALRVWLDPRKLAASGLTAADVYTALQDNNYLSAIGT
                     TKGQMVSVDLTAGTDLHSAEEFKNLVVKQKNGALVRLQDVGNVVLGAEEYDSDVAFKG
                     KRSVFIAINVAPNANILTVMADIRKIFPEIQAQMPAGLHGEIGYDATTYVNAAIRDVV
                     KTLLETLIIVTLVIFLFLGSVRSMIIPTLAMPLSLLGAFFVMLTLGYSINLLTLLALV
                     LAIGLVVDDAIIVVENVDRHMKLGQPPLQAALLAARELAAPIIAMSVVLVAVYVPIGF
                     RGGLTGALFSEFAFTLAGAVAVSAVIALTLSPMMCARFLKPEHGSNRLVRLIDRNFEW
                     FTNLYHRMLRRILDSWKVVVTFGLVVIALITLMLTIPVAGVVAKSELAPAEDQGVLFY
                     LSTGAPDSSIQQMATYQRQAFQELSALPEYANSFQF"
BASE COUNT          661 a         1051 c          997 g          622 t
ORIGIN      
        1 gccgaggcct taaatcagca ggatctggcc acgcaagctg ccgcggatac cctcagcgtt
       61 tcgcgcaccc ggtacgcgct gggctccatc agcgcgctcg agctcttggt ggtgcaacgc
      121 aattaccagc aggcgcgcat cgcccaggtg caagcgcagg cggcacgcta cgccgacact
      181 gccgcactgt tccaggcgct aggcggcggc tggtggaatc ggccgccaag caaagccacg
      241 ccgactgctg cggccgggcc taactgaaac cgcattcgcg atcgctacgg gacattaggt
      301 tttggaacgg acaaccctgc aacgttggcg gccattgatc gtggctgtca ttgctctcgt
      361 actcgtcatc ggaggcgtaa agggcttcgg cgtctataaa atgatgcggg gcttcgcggc
      421 gcaggaaccg cagaagttca cggtgagcac cgcgaaggcg gagtaccggg actggcagcc
      481 gaaacttacc gccgttggca gtgtgcgggc tgagcgcggc actgatctca gcgcggaagt
      541 ggccggtatc gtcgaggcca tccagttcga atccggtgac gacgtcaagg caggccaact
      601 gctgctgcag ttgcgatctg cggatgaggt cgcccacctc gaatccctca aggcgaatgc
      661 cgtgctggct gagaccacgt accaccgcga cgaggcgcag ttcgcagcgc aggcaatcag
      721 tcaagccacg ctggataccg atgcagccaa cctgcgtggt accaaagccc aggtggcgga
      781 acagcaggcg ctcgtggaca agaaatttgt gcgtgcgccg ttcgccggac acctcggggt
      841 gcgcgcggtg gatctgggtc agtacctctc gcccggcacg aagatcgtga ccctgcagca
      901 gctcgatctg atctacgtcg acttcttcct tccgcagcag gacctgagcc aggtgaatgt
      961 cggccaaaaa gttcaggcct cctccgacac ctactctggt ctgcgctttg cgggcgagat
     1021 tctggcgatc gatccactgg tcgacgtcaa tacgcgcaac gtgcagatcc gggccgcgct
     1081 caagaacccc gggcataagc tattgccggg catgtccgcc agcgtcgata tcgaggttgg
     1141 cggcccgcag cggtacatca cgttgcccca gacagcgatt agctacaacc cctatggcgc
     1201 gacggttttc atcatagtgc cccagagcgc cgtcgcgtct aaggacggcg gtaaagagaa
     1261 gtcgggcggc gaaacttctt ccgcggcgca gcagagcccg cctttcaagt cagacggtca
     1321 cgatctggcc gcaaaacagg tgttcgtcac gaccggggca acgcgcggcg atcaggtggc
     1381 gatactgaag ggcgtcactg aaggtgacca ggtcgtcaca agtggccaga tcaagctcaa
     1441 aaacggcgcg cttgtcacta tcaacaacga cgtgcagccc tccagcgatc cgaaccccgc
     1501 tccgtcggaa cagtagaacg gagtatgaac gttaccgatc tcttcatccg gcggccggtg
     1561 ctggctacgg ttgtcagtct cctgatactg ctgcttggaa tacgcgcgct cggcgccctg
     1621 ccgattcgtc agttcccaaa gaccgagaac gccaccgtca cggtaactac ccagtactac
     1681 ggcgcgagcg ccgatctcat cgccggcttc atcacgacgc caatagaggc cgccgtagca
     1741 gaggcgcagg gcatcgacta cctgacctcg cagagcagca acggcgtgtc cacggtcact
     1801 gcaacgctgc gcctcaacta cgattccaac cgggctctgg cggagatcaa caccaaggta
     1861 aactcggtgc tgaaccaatt gccgaccggc acccagcagc cggtcatcag cgtgcaagtc
     1921 ggcgaaaccg ttgcgggcat gtacctgggc ttcaatagcg ataccctgcc gcccaaccag
     1981 atcaccgact atgtgatccg catcgttcag ccgaaactgc agaccatagc tggtgtgcag
     2041 aacgccgaac ttctcggcgg ccgccagtac gcgctgcgcg tctggctcga tccacgcaag
     2101 ctcgcggcga gcggcctgac tgccgcggac gtgtataccg cgctacagga caataactac
     2161 ctgtcggcga tcggcacgac caagggccag atggtcagcg tcgatctcac cgccggtacc
     2221 gacctgcact cggccgaaga attcaagaat ctcgtcgtca agcaaaagaa tggggcactg
     2281 gtgcgcttgc aggatgtcgg caatgtcgtg ctcggcgcag aagagtacga ctccgatgtt
     2341 gccttcaaag gcaagcgctc ggtgtttatc gccatcaacg tggcgccgaa tgccaacatc
     2401 ctcaccgtga tggccgacat ccgcaagatc ttccccgaaa tccaggcaca gatgcctgcg
     2461 ggcctgcatg gcgagatcgg ctatgacgcc accacctatg tcaacgccgc gatccgcgac
     2521 gtagtgaaga ctctgctgga gactctgatc atcgtcacgc tggtgatctt cctgttcctc
     2581 ggcagcgtgc gctcgatgat catcccgact ctcgccatgc cgctgtcctt gctcggcgcc
     2641 ttcttcgtca tgctgacact gggttacagc atcaaccttc tgacgctgct ggctctggtg
     2701 ctcgcgatcg gtctggttgt ggacgacgcc atcattgtcg tcgagaacgt cgatcgccat
     2761 atgaagctcg ggcaaccgcc cctgcaggcg gccctccttg cagcgcggga gctggcagcg
     2821 ccgatcatcg cgatgtccgt ggtgctcgtc gcggtctacg ttcccatcgg cttccgcggc
     2881 ggcctcaccg gagcgctgtt ctccgaattc gccttcacgc ttgccggagc ggtggcggtg
     2941 tcagcggtga ttgcactgac gctgtcgcca atgatgtgcg ctcgttttct taagccagag
     3001 cacggcagca accgtctggt gcggctcatc gaccgcaact tcgagtggtt cacgaacctc
     3061 taccacagaa tgctgagacg cattctggac tcctggaaag tagtggtgac ctttggcctg
     3121 gtggtcatcg cgctgatcac gctgatgctg acgatcccgg tggcgggtgt ggtagccaag
     3181 agcgagctgg cgccggctga ggaccaggga gttctctttt acctgagcac cggtgcgccg
     3241 gattcctcga tccagcagat ggccacctat cagcgccagg cgtttcagga attgagcgcc
     3301 ctgcccgagt acgcaaattc gttccagttc g
//
LOCUS       DATMRM010000191         3835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_437068, whole genome shotgun sequence.
ACCESSION   DATMRM010000191 DATMRM010000000
VERSION     DATMRM010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437068"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VNZ06_05625"
     CDS             complement(<1..955)
                     /locus_tag="VNZ06_05625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWW20263.1"
                     /translation="MRFEDSSRVIAPPRLSVIVPVFNEQDSIDPLYRAIVHGVEPLGI
                     PFEIVLVDDGSTDATVAHAVNLARADPRMRIVKFRRNYGQTAAMAAGVAHAAGAILIT
                     MDGDLQNDPADIERFLEKIEQGFDIVVGWRHERQDKLLTRRIPSRIANWIIGKVTGVP
                     IRDNGCSLKAYRASVIKAIPLYSEMHRFIPAMASIAGPRIAEIKVRHHARRFGKSKYG
                     LSRIYKVLLDLLVVKTVISFTSRPLLWFTLLSLPAFFFALLGFGYSAYAVLINGERLP
                     LPVAGSGFIFGACAVMLMSGGAIGELVFRLGDVRERDFARLT"
     gene            1351..2100
                     /locus_tag="VNZ06_05630"
     CDS             1351..2100
                     /locus_tag="VNZ06_05630"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWW20264.1"
                     /translation="MRRRGLPLLKNLPSYSRCVLVAGCQRSGTTMLARLIAAAPGFAR
                     LRLTADDELDAALALAGFVALPNDPRYCFQTTYLNECFLEYRRLAPDQRLIWVVRNPY
                     SVVYSMLYNWRRFALNELYEGCGVASAKSVRLRRSRLMWPFGPSRIEKACLAYRGKVA
                     QILSIRHLLRPEQLLTIDYDLLSAAPEQWLPRIFQFADAPYDAACAYEVRRGPAKKAD
                     ALSIRARRLIEQLTGPTYRECIALAAGHDVA"
     gene            2113..3666
                     /locus_tag="VNZ06_05635"
     CDS             2113..3666
                     /locus_tag="VNZ06_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HWW20265.1"
                     /translation="MPLYSREKARRSLLNTVGYRAISQVATLAGYVVLVRSLSEHSFG
                     IYSLLYAIIPVISTAASLGLEQTLRRFQPEYLRVGDASAAAWLLRVVMIARFSSNAVI
                     LALLFVAWNVVAPIFGLGPYRADFALFCLLVLLYFQGRILEFALASHMLHRFGVGSTV
                     LLAVTKLLAYAILAALHALSLRSAILVDTLAYGLAYVFMRTAYRYAASGARTRAAYRL
                     PATERTRLLRYALYNNFNDAGSILLYVQTDNFFIAALLNPVAVGAYAFYTRVNAMVSS
                     LTPMRLFENVVQPLFFATPAQQASDRIPRYFTLLLNCSLLAQLPIVAYATVYHGEIVG
                     LLLGGKFQDLSWLMPVIIAFGTTSNVIAIPVTNVAQYHERAALILGSQLFGIYQIGCM
                     ILLVPYIGLLGAAVATGTYHLFRNLFVWWQVRRDARWLNFPAVVTCATLVWGGAILAC
                     SELKRVIAAPPLAAMACGVVVCGLAALVYVRTPALSRSDRELLAQVFHGREARLLHRL
                     GVLRPLGHA"
BASE COUNT          668 a         1187 c         1198 g          782 t
ORIGIN      
        1 cggtgagacg cgcaaagtcg cgctcgcgaa catcgcccag cctgaagacc aattctccga
       61 tagcgccgcc cgacatcagc atgaccgcgc aggcaccgaa tatgaagccg gagccggcga
      121 ccggcagcgg caagcgctcg ccgttgatca gcactgcgta ggcgctgtaa ccgaacccca
      181 acagggcgaa gaaaaatgcc ggcaagctga gcaaggtgaa ccacagcagc ggccgcgatg
      241 tgaaactgat aaccgtcttg accaccagca ggtcgagcag caccttataa atgcgcgaca
      301 acccgtactt ggacttgccg aagcggcgag catgatggcg aaccttgatc tcggcgattc
      361 gcggacccgc aatggacgcc atcgccggta tgaagcggtg catctccgag tacaggggaa
      421 ttgccttgat gaccgaggca cggtacgcct tgagggagca gccgttgtcc ctgatgggca
      481 ccccggtgac cttgccgatg atccagttgg caatgcgcga agggatcctg cgcgtcagca
      541 gcttgtcctg gcgctcgtga cgccagccga cgacgatatc gaaaccctgc tcgatcttct
      601 cgaggaagcg ctcaatgtcg gccggatcgt tctgcaagtc gccgtccatc gttatgagaa
      661 tcgccccggc ggcatgggcg acaccggcgg ccatggccgc ggtctgtccg taattgcgcc
      721 ggaacttgac gattcgcatg cgcggatcgg cgcgcgccag attcactgca tgcgcgaccg
      781 ttgcgtccgt gctgccatcg tccacaagaa caatctcgaa aggaattccc agtggttcga
      841 ccccgtggac aatcgcccgg taaagcgggt cgatgctgtc ctgttcgtta aaaacgggga
      901 cgataaccga gagcctcggt ggggcgatga cgcggctcga atcctcaaat cgcatcggtg
      961 cgcgcaagtc cctctcggtc cgatcggtcg tggtggcagc gacctgccgg gcaactccac
     1021 ataataaggc gaacggatcg gaccggcggg catcgatatg acctgaaaac cgtcgctcgc
     1081 cttcagctcg tgtacaggga aatatagcac agaagccatc agcgcctggc ggtgctcgcg
     1141 gggatcgtgc ggagcgttcg cgcctgcttg caggcttaag accgatgtgc ttccatcgcc
     1201 gtgaaccagc tgcgcagcag ccggtcagca cgagttatgg tcaacgagaa taattacgtc
     1261 gtccgcggga cgattacaag gacgtccata ccacagactc aacgggaatg gctcgatcgt
     1321 tacagaacct ggcgacgatt ccaccacggc gtgcgcaggc gcgggctgcc gctgttgaag
     1381 aatctcccga gctattcgcg ctgtgttctg gtggcgggat gtcagcgctc cgggacgacg
     1441 atgctggcgc gcctgatcgc ggccgcgccc gggttcgcgc gcttgcgcct cactgcggac
     1501 gacgagctgg acgctgcact ggctctggcg ggtttcgtcg ctcttccgaa tgacccacgc
     1561 tactgctttc agacgaccta cctgaacgag tgtttcctcg aataccgccg gctggcgccc
     1621 gatcagcgac tgatctgggt agtgcgcaat ccgtactcgg tcgtgtattc gatgctgtac
     1681 aactggcgac ggtttgccct caacgagttg tacgaaggct gcggggttgc cagtgccaaa
     1741 tcagtgcgcc tgcgccgctc gagactgatg tggccgttcg gcccgtcacg cattgagaag
     1801 gcatgtctcg cctaccgcgg caaggtagcg cagattctct cgatcagaca tctgctgcgg
     1861 cccgaacaat tgctcaccat cgactatgat ctcctgagcg cagcgccaga gcagtggctg
     1921 ccacggatat tccagtttgc cgacgcgccc tatgatgctg cctgcgcgta cgaggtgcgg
     1981 cgcggcccgg ccaagaaggc cgatgcgcta tcgatccggg cgcggcggct gatcgagcag
     2041 ctcaccgggc cgacgtaccg ggagtgcatt gccctggcgg ccggccacga cgtcgcttga
     2101 gtgctacggg cgatgccgct ttacagcaga gagaaggcgc gccgctcgct tctcaatacc
     2161 gtcggctatc gcgcgatctc gcaggtcgcc actctggccg gttacgtggt gctggtacgc
     2221 tcgctttccg agcactcgtt cggcatctac agccttctct acgcaatcat tcccgtcatc
     2281 agcactgctg catcgctcgg cctcgagcag acgctgaggc gttttcagcc cgagtacctg
     2341 cgcgtggggg atgcgtcagc ggccgcctgg ctgctgcggg tggtgatgat cgcgcgtttc
     2401 tcgtccaacg cggtgatcct cgcgctgctg ttcgtggcgt ggaacgtcgt ggcgccgatc
     2461 ttcggcttag gtccttaccg cgccgacttc gcgctctttt gcctgctggt gctgctgtat
     2521 ttccaggggc gcatcctgga attcgcgctg gcgtcgcaca tgctgcatcg ttttggagtg
     2581 ggctcgaccg ttctgctggc ggtgaccaag ctgctcgcct acgcgatcct ggcggcgctg
     2641 catgcgctct cgctgcgcag cgcgattctc gtcgatacgc tcgcttacgg actcgcctat
     2701 gtgttcatgc gcaccgccta ccgctacgcg gcgagcggcg ctcgcaccag ggcagcttat
     2761 cggttgcccg ccaccgagcg cacgcgactg ctgcgctacg cgctgtacaa caacttcaac
     2821 gacgcaggct cgatcctgct gtacgtgcaa actgacaact ttttcattgc agcgcttctc
     2881 aatccggtgg ccgtcggcgc ttacgccttc tatacgcgcg tcaatgcgat ggtgtcgagc
     2941 ctgacgccca tgaggttgtt cgagaacgtg gtgcagccgc tgtttttcgc gactcccgcg
     3001 cagcaagcgt cggatcgcat tcccagatat ttcacgctat tgttaaactg cagcctgctc
     3061 gcgcagctgc ccattgtcgc gtatgctacc gtgtatcacg gcgagatcgt cggactgctg
     3121 ttgggcggca agttccagga cctgtcgtgg ctcatgccgg tcattattgc gtttgggacc
     3181 acctccaacg tcatcgcgat tccggtgacg aatgtggcgc aatatcatga acgcgcggcg
     3241 ctcattttgg ggagccagct gtttggcatc taccagatcg gttgcatgat attgctcgtt
     3301 ccctatatcg ggttgctcgg cgccgcggtc gcgaccggta cctatcacct gttccgaaac
     3361 ctctttgtgt ggtggcaggt gcggcgtgac gcgcgctggc tcaacttccc ggccgttgtg
     3421 acctgcgcga ctctggtctg gggcggtgcg atcctcgcat gttccgagct caagagggtg
     3481 atcgctgcgc cgccgctcgc tgcaatggcg tgtggcgtcg tagtgtgcgg actcgccgcg
     3541 ctggtgtatg tgcgaactcc ggcactttcg cgctccgacc gcgagctgct ggcgcaggtg
     3601 tttcacggcc gcgaggcacg actcctccac aggctcggag tgctgcggcc gcttgggcat
     3661 gcgtgacgtg ccggccgcgg aggtgaatcc ggctgaacgg ctcagagcgt ttgggaatcg
     3721 tgaatgatcg caggggttcc cgacagtctg gaatcgcgag ccggtgaagc gcaggcgagc
     3781 gctgctcaga tcatgagcag gccggttatc cgcatgctgc ttttcacgcg cgagt
//
LOCUS       DATMRM010000192         3927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_437117, whole genome shotgun sequence.
ACCESSION   DATMRM010000192 DATMRM010000000
VERSION     DATMRM010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437117"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1791
                     /locus_tag="VNZ06_05640"
     CDS             <1..1791
                     /locus_tag="VNZ06_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HWW20266.1"
                     /translation="EFGSASASAEGFRNESSSAAASLYRRPWGIKGLDASDLFARATD
                     GASWLAISGNPLAPATQSRAGDLLRALSAGDRGVLRDLDGWFAIAWWDADRQSLNLIR
                     DRFGSEPLFYASTDRGLLFGSRARDLVGVGGLRKRLSAQGVVQFLSYCYLPGVDTMYE
                     GMLRVPAGSHLQFSARDRSIRIDRWYRLSYADPVPPDEPAIEKQYRELLEQAVTRRLG
                     GGRTGVFLSGGMDSSSAITFARRHMTDSINSFGFRCSGGSFDESHYARSLADELHAKH
                     SEVEYGENESLSILDAIGEMEVPFCDVGIEIGTWLLSRAASGQVDYILTGDGGDEIWA
                     SHPVYAAQKLMKAYDRLPLPMLMRRGVAGMTSWVRDSDQKRNLPVVLKRLIPPPSIPR
                     ELGHFRWRTYFTRETMQNLLAGSTRQQAASYDPYQVIRTSLEGYDGPDDNISPWLYSD
                     YTTASAFYFSRLLFARSFGVEVRTPFYDRDLVEYGARIPSFKKLEGMERTKRLFRASM
                     EGILPDVINHRKDKLGHSVPLKNWLRTTGKLGQEVYATLTSPQMRDRGLVQPEAVTRL
                     WDEHLARRENHSHRLWSLFVLEHWLRRRFD"
     gene            complement(1788..3713)
                     /gene="asnB"
                     /locus_tag="VNZ06_05645"
     CDS             complement(1788..3713)
                     /gene="asnB"
                     /locus_tag="VNZ06_05645"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HWW20267.1"
                     /translation="MCGIAGIVGPGFCNPENLGRMLAAISHRGPDDEGTYLDDHAALG
                     HRRLSIIDLSSGHQPLRDIRGSLWLIANGEIYNYRELRRDLEQRGHQFITNSDCETIL
                     HLYREYGERCLEHLRGMFAFALWDKEKRQLLIARDHLGQKPMFYAEQNGYLAFGSEIK
                     ALLAFDPSLRKLNLEALDQYLSLRLIGSPRSMFKGVQKLPPGHALMVRPGEKARVWRY
                     WDIRYAPKLQGSEDELTDALEAELLVALRLHLASDVPVGAFLSGGLDSSLIVAMLARH
                     CVAKGFPTFTMSVPHKTFDEAPYARQVAEQYQTEHHEGTLTPNLAESLPKLVWHLDEP
                     SDPLALCTYELAEFTRRYVKVVIGGDGGDELFGGYDRYYGYTYADRYAILPQALRRHV
                     VGPMLKLVPTGGWYKSRGHQMRWLHQMALKQGGARYAASLSYFYFDAAARRELLTDRS
                     REALGSFDPEDCIREPFDRVQAEPLDRMLYADAEIRMPDHPVMITDRMTMAFGLEARS
                     PFMDHVMAQFAARLPCDVKVRGTSLRYLQRRLASRYLTESLMQRPKQGFASAMPYLLQ
                     TEYRRLYSALLPSSQLVSDGIIRSEPMRRLVAEHLTGKADHGNRLWQLLNAEIWYRMQ
                     IKQTPAEQISVDLRPTG"
     gene            complement(3732..>3927)
                     /locus_tag="VNZ06_05650"
     CDS             complement(3732..>3927)
                     /locus_tag="VNZ06_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20268.1"
                     /translation="FVLESTLRPLGIQPPLTQRRLDFFTKSFLFSTAKYRSLLGFTPQ
                     IPFDVGAARTIEWYREAGYL"
BASE COUNT          735 a         1170 c         1232 g          790 t
ORIGIN      
        1 gagttcggct cagcgtcggc gtccgcggaa ggttttcgca acgaaagctc gagcgctgct
       61 gcgagcctct atcgtcggcc ctggggtatc aagggcctcg acgcatccga tctgttcgct
      121 cgtgccacgg acggcgcctc ctggctggcg atcagcggta acccactcgc tcctgcgacg
      181 caatctcgtg ccggagatct gctacgcgcc ctcagcgccg gcgatcgcgg cgtgttgcgc
      241 gatctcgacg gctggtttgc gatcgcatgg tgggatgcgg accgtcagag tctgaacctg
      301 attcgcgatc gcttcggcag cgagccgctg ttttatgcga gcaccgaccg cggtttgctg
      361 tttggctcgc gagcgcgtga tctggtgggt gtcggtgggc tgcgcaagcg cctgtccgct
      421 cagggtgtcg tgcagtttct gagctactgt tacctgccgg gcgtggatac gatgtacgag
      481 ggcatgctgc gcgtgccggc gggcagtcat cttcagttct cggcgcgaga tcgttcgatc
      541 agaatcgatc gttggtatcg gctcagctac gccgacccgg tgccccctga cgagccggcg
      601 atcgagaagc aataccgcga gctgctcgag caggcggtga cgcgccggct cggcgggggc
      661 cgcacgggtg tatttctctc cggtggcatg gattccagct cggccattac cttcgcgcgc
      721 cgacatatga cggactccat caactctttt gggttccgtt gttccggcgg ctcgttcgat
      781 gaatcccact atgcgcgttc gctggccgat gagctgcatg ccaagcattc cgaggtcgaa
      841 tacggcgaga atgaatcgct gagcattctc gatgcgatcg gagagatgga ggtgcccttc
      901 tgcgatgtcg gcatcgagat cggcacgtgg ttgctgtcgc gcgcggcttc gggacaggtc
      961 gattacatcc tgaccgggga cgggggcgat gaaatctggg ccagccatcc ggtctacgcc
     1021 gcacagaaac tgatgaaggc ctacgaccgc ttgccgttgc cgatgctgat gcgtcgcggt
     1081 gttgccggaa tgacttcatg ggtgcgcgac tcggatcaaa aacgcaacct gccggtggta
     1141 ttgaagcggc tgatcccgcc gccctcgatt ccgagggagc tgggtcactt tcgctggcgc
     1201 acctatttca cgcgcgaaac gatgcagaat ctgctggctg gatcgacgcg tcaacaggct
     1261 gcgtcttacg atccgtacca ggttattcgc acctcgctcg agggttacga cggcccggac
     1321 gacaacatct cgccgtggct gtattcggac tacaccacag cgagtgcgtt ctatttttcg
     1381 aggctattgt tcgcgcgttc gttcggagtc gaagtgcgca cgccgttcta cgaccgcgat
     1441 ctggtggagt atggtgcgcg catcccttcg ttcaagaaac tggagggtat ggagcgcacc
     1501 aagcggctgt tccgcgcctc gatggaaggc atcctgccgg acgtcatcaa tcaccggaaa
     1561 gacaaactgg ggcactcggt ccccttgaag aactggctgc gcaccaccgg caaactcggt
     1621 caggaagttt acgcaacgct taccagcccg caaatgcgcg atcgtgggct ggtgcaaccg
     1681 gaggcggtca ctcgattgtg ggatgagcac ctggcgcgac gcgagaatca ctcgcaccgc
     1741 ttatggtcgt tgttcgtgct cgaacattgg ttgcggcggc gcttcgacta accggtcgga
     1801 cgcaaatcga cgctgatctg ttccgccggc gtctgtttga tctgcattcg ataccagatc
     1861 tccgcattca gcagctgcca gagtcggtta ccgtgatcgg ctttgcccgt gaggtgctcg
     1921 gccaccaggc gcctcatcgg ctccgagcgg atgataccgt cggaaacgag ctgagagctc
     1981 ggtagcaggg cggagtatag gcggcggtac tccgtctgca atagatacgg catcgccgaa
     2041 gcgaagccct gcttcggccg ttgcatcagc gattcggtca gataacgcga cgccaggcgg
     2101 cgctgcaggt agcgcaggct cgttccgcgc accttcacat cgcagggcag ccgtgcggca
     2161 aactgcgcca tcacgtggtc catgaacgga ctgcgcgcct cgagaccgaa tgccatcgtc
     2221 atccggtcag tgatcatgac cgggtgatcc ggcatgcgga tctccgcatc cgcgtagagc
     2281 atgcggtcca gtggctcggc ctggacgcgg tcgaagggtt cgcgaatgca gtcctcggga
     2341 tcgaagctgc caagggcctc gcggctgcgg tcggtgagca gttcgcggcg cgcggcggca
     2401 tcgaaataga aataggacag gctcgcggca tagcgggcgc caccctgctt gagcgccatc
     2461 tggtgcaacc agcgcatttg atggccgcgg ctcttgtacc aaccacctgt aggcaccagc
     2521 ttgagcatcg gccctacaac gtgcctacgc agggcttgcg gcaagatcgc gtaacgatcg
     2581 gcgtaggtgt aaccgtagta gcggtcgtag ccgccaaaga gctcatcacc gccgtcaccg
     2641 ccgatgacaa cttttacgta gcggcgtgtg aactctgcca attcataggt acacaacgcc
     2701 agcggatcgg agggttcgtc caggtgccac accagtttcg ggagtgactc ggccagattc
     2761 ggggtcagcg tgccctcatg atgctcggtc tggtattgct cggcgacctg gcgcgcatag
     2821 ggcgcctcgt caaaggtctt gtgtggcacg ctcatcgtga acgtagggaa ccccttcgcg
     2881 acgcagtggc gagccagcat cgcaacaatc agactggaat ccagaccgcc actgaggaat
     2941 gcgcccacgg gcacgtcgct cgccagatgc aggcgcagtg cgaccagcag ttccgcttcc
     3001 agcgcatcgg tcagttcatc ctctgaaccc tgaagcttcg gtgcgtagcg aatatcccag
     3061 tagcgccaga cccgcgcttt ctcgccgggc cgaaccatca aggcatggcc gggaggcagt
     3121 ttctgtacac ccttgaacat gctgcggggt gagccgatca gtcgcaacga cagatactga
     3181 tcgagtgcct cgaggtttag cttgcgcaga ctcgggtcga aggccagcaa cgccttgatt
     3241 tcggaaccga acgccagata gccgttctgc tccgcataga acatcggctt ctgcccgagg
     3301 tggtcgcgcg cgatcagaag ctgcctcttc tccttatccc aaagcgcgaa agcgaacatg
     3361 ccgcgcaggt gttccagaca gcgctcgccg tactcacggt acagatgcaa aatggtttcg
     3421 cagtcgctgt tggtgatgaa ctgatgaccg cgctgttcga ggtccctgcg cagctcgcga
     3481 tagttgtaga tctcaccgtt ggcgatcagc cacaatgagc cgcgaatgtc gcgcagtggc
     3541 tggtggccac tgcttagatc gatgatcgaa agccgacggt gccccagcgc cgcgtgatcg
     3601 tcgagatagg tgccttcgtc gtcgggccca cgatggctga tggccgcaag catgcggcct
     3661 aaattttccg ggttacaaaa cccgggtccc acaattccag cgattccgca cataagctcg
     3721 gtcgtgcctg ctcagaggta gcccgcctca cgataccatt caatggtgcg cgccgctccg
     3781 acgtcgaagg gtatctgtgg agtaaagccc aacaggctgc ggtatttggc cgttgagaac
     3841 aggaaggatt ttgtaaagaa gtccagccgt cgctgcgtca gcggcggttg aatgcccagc
     3901 ggacgaagcg tgctctccaa tacaaag
//
LOCUS       DATMRM010000193         5167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_736973, whole genome shotgun sequence.
ACCESSION   DATMRM010000193 DATMRM010000000
VERSION     DATMRM010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736973"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VNZ06_05655"
     CDS             <1..246
                     /locus_tag="VNZ06_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20269.1"
                     /translation="KEVAADALNLTAGRLRDLGLLDEVLPEPLGGAHRDAALMSNVLR
                     EGILRHLSQLQQLSIDELVAQREARIASFGVFNENPP"
     gene            243..1652
                     /gene="tilS"
                     /locus_tag="VNZ06_05660"
     CDS             243..1652
                     /gene="tilS"
                     /locus_tag="VNZ06_05660"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWW20270.1"
                     /translation="MSGRRPARPDAASELLSPPKRFDSAWLRGQLRSLIGTLRARSLC
                     LAYSGGLDSCALLCALASLRKREGFVLRALHVNHQLQPLAARWARQAQSNARRLRVPC
                     EILTVRVNRGRGESLEAAARTARYQALLSRLKPDELLLTAHHQEDQFETVLLALMRGS
                     GVRGLSAMNPVTPLAHTRLLRPLLPVARAQLERYLKRRAVGWSEDPSNTDERFDRNYL
                     RHVVVPLLRARWPAAAATAARSAAHLSQTRAMLERLGLAHLKNARDGQALRVSVLRRL
                     PQAERINALRVWLAERALPMPDYDRMREIAGPMLSARPDAMPAVSWQGAQLRRHADRL
                     LAYRARETHLPAAAGIEVERWSWRTQPWVSLGSAGSLGLVRDRHGDVRLAALPSVLSV
                     RYRRGGERLAGTQGRFALKDLLQRKGLAPWVREQVPLVMHDRTVVAVADLWLCPQFAA
                     HSGSSSGRARFRWRHPGVD"
     gene            1725..3371
                     /locus_tag="VNZ06_05665"
     CDS             1725..3371
                     /locus_tag="VNZ06_05665"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HWW20271.1"
                     /translation="MTRFVFVTGGVVSSLGKGIAAASLGAILEARGLRVAMVKLDPYI
                     NVDPGTMSPFQHGEVFVTADGTETDLDLGHYERFVRTTTGRNSNFTTGRIYERVIAKE
                     RRGDYLGATVQVIPHITDEIKRSIMLGAGDADVCMVEIGGTVGDIESLPFLEAIRQLG
                     VELGRTQALYMHLTLVPIVGGSGEIKTKPTQHSVKELRGIGIQPDVLLCRCRETLPDE
                     QRRKIALFTNVEERAVISAIDADDIYRIPVLLHEQNLDDFVVDKLRLEVPPADLNAWR
                     QVASAKTNPDGQIDIAMVGKYVQIRDSYISLNEALMHGGLKTRTRVKVHYIESTDVEK
                     EGVGCLAGMDAILVPGGFGERGIEGKIQAIRYAREQGVPYLGICLGMQLAIIEFGRHV
                     LMLPDAHSTEFNRSTASPVIALITEWQDRALGVQQRTESSQLGGTMRLGAQDVTLQAG
                     TRARDLYGRDLIGERHRHRYEFNNNYLDAYTRSGMRFSGFSRDGLVEIIELPSHPWFV
                     ATQFHPEFTSTPRDGHPLFTGFVRAARHYRAAQLPAAAGA"
     gene            3368..4201
                     /gene="kdsA"
                     /locus_tag="VNZ06_05670"
     CDS             3368..4201
                     /gene="kdsA"
                     /locus_tag="VNZ06_05670"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HWW20272.1"
                     /translation="MKLAGFEVGIDRPLFLIAGPCVIESEALVQEVAGTLKEITTALN
                     IPLIFKASFDKANRSSAASFRGPGLAAGLKALQSVQRQFKLPVLTDVHEDTPIAEVAS
                     VVDVLQTPAFLCRQTNFIRNVAAAGRPVNIKKGQFLSPWEMQNVVDKARSTGNSQILV
                     CERGFSFGYNNLITDMRALAIMRSTGCPVVFDATHSVQLPGGQGTASGGQREHIPVLA
                     RAAVAAGIAGIFMETHPRPEQALSDGPNAWPLKHMAALLTTLKQLDATAKAQPFEESL
                     L"
     gene            4198..>5167
                     /gene="eno"
                     /locus_tag="VNZ06_05675"
     CDS             4198..>5167
                     /gene="eno"
                     /locus_tag="VNZ06_05675"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HWW20273.1"
                     /translation="MSQITEIHAREIIDSRGNPTIEADVSLKSGAIGRAAVPSGASTG
                     SREAVELRDGEPRRFGGKGVAKAVDNVNTVLREALIGKEALDQAGIDARMIELDGTPT
                     KSRLGANALLAISLANAHAVARESGQPLFKYLGRSRTPVMPVPMMNIINGGAHADNSL
                     DIQEFMILPVGAPSLREAIRYGAEVFHALKKILHSRHLATAVGDEGGFAPDLPSNEAA
                     LQVILEAIDAAGYRAGRDIYLGLDVASSEFFKNGEYQFEAEHRSFTSAQFTQYLSGLV
                     AKYPIITIEDGMSEGDWDGWKLLTDALGRRIQLVGDDLFVTNTKILA"
BASE COUNT          908 a         1616 c         1728 g          915 t
ORIGIN      
        1 aaggaagtgg ccgccgatgc cctgaatctg acggcggggc gtctgcgaga cctggggctg
       61 ttggatgagg tgctgccaga gccgctcggc ggagcgcacc gcgacgcggc cctgatgagc
      121 aatgtgctgc gtgaaggcat cctccggcat ctgagccagc tgcagcagct cagcatcgac
      181 gagctggtcg cgcagcgcga ggcacggatc gcttcgttcg gagtattcaa cgagaatccg
      241 ccgtgagcgg gcgccgcccg gctcgcccgg acgcggccag cgaactgctc agcccaccga
      301 agcgattcga ttcggcctgg ctgcgcgggc agctgcgctc gctgatcggc acgctgcgcg
      361 ctcgcagctt gtgcctcgcc tattccggcg ggctggattc ctgcgcactg ctgtgcgcac
      421 tggcgagtct gcgcaagcgc gagggctttg tgctgcgagc gctgcatgtc aatcatcagc
      481 tgcagccgct cgccgcccgc tgggcgcgcc aggcgcagtc caacgcccgg cgcctgcggg
      541 ttccgtgtga aatactcacg gtgcgtgtca atcgtggtcg cggcgaatcg ctcgaggccg
      601 cggcgcgtac cgcacgctat caggcactgc tgtcgcggct gaagccggat gagctgctgc
      661 tcactgcgca tcaccaggag gatcagttcg agaccgtgct gctggcgctg atgcgcggca
      721 gcggcgtgcg cggcctgtcg gcgatgaatc cggtcacccc gctggcgcac acgcggctgc
      781 tgcggccgct gttgccggtt gcgcgcgcgc agctcgagcg ctacctgaag aggcgcgccg
      841 tcgggtggag cgaggacccc agtaacaccg atgaacgatt cgatcgcaat tatctgcgcc
      901 acgtggtcgt gccgctgctg cgggcgcgct ggccggccgc ggcagcgacc gccgctcgca
      961 gtgccgccca tctgtcgcaa acgcgcgcga tgctggagcg actcgggctg gcacacctga
     1021 agaacgcgcg cgatggtcag gcactgcgtg tgagcgtgct gcgccgactg ccgcaggcag
     1081 agcgcatcaa tgcgctgcgt gtctggctcg ccgaacgggc gctgcccatg cccgactatg
     1141 accgcatgcg cgagatcgcc ggccccatgc tgtcggctcg gccggatgcg atgcccgcgg
     1201 tgagctggca aggcgcgcaa ctgcgccggc acgctgaccg cctgctggca taccgtgcac
     1261 gcgagacgca tttgccggcg gcggcaggga tcgaagtcga acgctggagc tggcgcacgc
     1321 agccctgggt gtcgctcgga tcggccggct cgctcggact ggtgcgcgac cggcacggcg
     1381 atgtgcgctt agccgcgctg ccgagcgtgc tcagcgtgcg atatcggcgg ggcggcgaac
     1441 gtctggccgg aacgcagggg cgcttcgcgc tcaaggatct gctgcaaaga aaggggcttg
     1501 cgccgtgggt gcgggagcag gtgcccctgg tcatgcacga tcgcacggtg gtcgcggttg
     1561 cggacctgtg gctgtgcccg caattcgccg cgcactccgg atcgagctcc ggtcgggcgc
     1621 ggttccgatg gcggcacccg ggcgtggatt gagcgcgcgc ctccgatttg atagtctgat
     1681 tgcccgtcgt cattggcgcg tctctcaggc ggcggtaatc ctcgatgact cgatttgtat
     1741 tcgtgaccgg cggtgtcgtt tcctcattgg ggaagggcat cgccgccgca tcgttgggcg
     1801 caattctcga ggcccgcggc ctcagggttg cgatggtcaa actggatccc tacatcaacg
     1861 tggatcctgg gacgatgagt ccgttccagc acggggaggt gttcgtcacg gccgacggaa
     1921 ccgaaaccga cctggatctc ggccactatg agcgcttcgt gcgcacgacg accggccgca
     1981 acagcaactt cacgaccggc cgcatttacg agcgcgtcat cgccaaagag cgtcggggtg
     2041 actatctggg agcgacggtg caggtcattc cgcacatcac cgacgagatc aagcgcagca
     2101 tcatgctcgg cgcaggcgat gcggatgtct gcatggtgga gatcggcggt acggtcggtg
     2161 acatcgagtc gctgccattt ctggaggcga tccgccagct cggagtcgag ctggggcgca
     2221 cccaggcgct gtacatgcat ctgacgctgg ttcccatcgt cggcggatcc ggcgagatca
     2281 agaccaaacc cacacagcac tcggtcaaag aactgcgcgg gatcggtatt cagcctgacg
     2341 tgttgctgtg ccgctgccgc gagaccctgc ccgacgaaca gcggcgcaag atcgcgctgt
     2401 tcaccaacgt tgaagagcgc gccgtgatat cagcgatcga cgccgacgac atttaccgca
     2461 ttccggtgct gctgcatgaa cagaatctgg atgatttcgt ggtcgacaag ctgcggctgg
     2521 aagtgccacc ggcagacctg aacgcctggc gacaggtcgc cagtgccaag accaatcccg
     2581 acggtcagat cgacatcgcg atggtcggca aatacgtgca gatccgagat tcttatatct
     2641 cgctgaacga ggcgctgatg cacggcggtt tgaagacccg cacgcgtgtc aaagtgcact
     2701 acatcgagtc caccgacgtc gagaaggagg gcgtcggctg cctcgccggc atggatgcca
     2761 ttctggtgcc cggcgggttc ggcgagcgag gcatcgaagg caagattcag gcgatacgct
     2821 atgcgcgcga gcagggcgtg ccgtatctcg gcatctgttt aggcatgcag ctggccatca
     2881 tcgagtttgg ccgccatgtg ctgatgctgc cggatgcgca cagcaccgaa ttcaatcgca
     2941 gcacggccag tccggtgatc gcgctgatca ccgagtggca ggaccgtgcg ctgggtgttc
     3001 aacagcgcac ggagtcttcg cagctcggcg gcaccatgcg cctcggcgcg caggacgtga
     3061 cgctgcaagc gggcacccgc gcccgcgatc tgtatggacg cgatctgatc ggcgagcgcc
     3121 atcgtcatcg ctacgagttc aataacaatt atctggacgc ctatacccgc tcggggatga
     3181 ggttttccgg attctcacgc gacggtctgg tggagatcat cgagctaccc agccacccct
     3241 ggttcgtcgc cacgcagttc catccggagt tcacctcaac accgcgtgac ggccatccgc
     3301 tgttcacggg cttcgtacgc gccgcccgcc actatcgcgc tgcgcagttg ccggccgcgg
     3361 ccggcgcatg aagctagccg gtttcgaggt cggcatcgac cgaccgctct tcctgatcgc
     3421 aggaccctgc gtgattgagt ccgaagcgct ggtgcaggaa gtcgccggca ccctcaagga
     3481 gatcactacg gcgctgaaca ttccgctgat attcaaggct tcgttcgaca aggccaaccg
     3541 ctcatcggcc gcgagtttcc gcgggccggg actggcggcg ggactgaagg cgctgcagtc
     3601 ggtgcagcgt cagttcaagc tgccggtact gacagacgtg cacgaggaca ctcccattgc
     3661 cgaagtcgcc tcggtggtcg atgtgctgca gacaccggca tttctgtgtc ggcagaccaa
     3721 tttcattcgc aacgtggccg ccgccggccg tccggtcaac atcaagaagg gtcagtttct
     3781 ctcgccgtgg gagatgcaga acgtcgtgga taaggcgcgc tcgaccggta attcgcagat
     3841 cctggtgtgc gagcgcggct tcagcttcgg ctacaacaat ctgatcaccg atatgcgcgc
     3901 gctggccatc atgcgcagca ccggctgccc ggtggtgttc gatgccaccc actcggtgca
     3961 gcttccgggc gggcagggca ctgcctccgg cggtcagcgc gaacatatcc cggtgctggc
     4021 acgggccgct gtggccgccg gaattgccgg cattttcatg gagacccatc cgcgcccgga
     4081 gcaggcgctg tcagatggac cgaatgcgtg gccgctgaag cacatggcgg ccttgttgac
     4141 gaccctgaaa cagctggacg cgaccgcgaa ggcgcagccg ttcgaggaat ctctgctatg
     4201 agtcagatca ccgaaatcca cgcgcgcgaa atcatcgatt cgcgcggcaa tccgacgatc
     4261 gaggcggacg tcagcttgaa gtcaggtgcg atcggacgcg ccgcggtgcc ttcgggcgcg
     4321 tcgaccggct cgcgtgaagc ggtggaactg cgtgacggtg agccgaggcg ctttgggggt
     4381 aagggggtcg ctaaggccgt ggacaacgtc aacacggtgc tgcgcgaggc gctgatcggc
     4441 aaggaggcgc tggatcaggc gggtatcgat gcgcgaatga ttgagctgga tggcacgccg
     4501 accaagagcc gcctgggcgc caatgcgctg ctggcgatct cgctggccaa tgcccatgcg
     4561 gtggcgcgcg aatccggcca gccgctgttc aagtatctcg gccggtcgcg cacgccggtg
     4621 atgcccgttc cgatgatgaa catcatcaac gggggtgcgc acgctgacaa cagcctcgac
     4681 attcaggagt tcatgatcct gcccgtcggt gcaccgtcgt tacgcgaagc gatccgctac
     4741 ggcgccgaag tgtttcacgc gctcaagaag atcctacact ccagacacct ggccaccgcg
     4801 gtcggtgacg aaggcggatt cgctccggat ctgccgtcga atgaagccgc gctgcaggtg
     4861 attctggaag ccatcgacgc cgccggttac agggctggac gcgatattta tcttggcctc
     4921 gacgtcgcca gctccgaatt cttcaagaac ggcgagtacc aattcgaggc cgagcaccgc
     4981 agcttcacgt cggcgcaatt cacccagtac ctgtccggtc tcgtagccaa atatccgatc
     5041 atcaccatcg aagacggtat gagcgaaggt gactgggatg gctggaagct gttgaccgat
     5101 gcattgggac gcaggattca gctggttgga gacgatctgt tcgtgaccaa caccaagatc
     5161 ctggccg
//
LOCUS       DATMRM010000194         8410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1967141, whole genome shotgun sequence.
ACCESSION   DATMRM010000194 DATMRM010000000
VERSION     DATMRM010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8410
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967141"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VNZ06_05680"
     CDS             complement(<1..767)
                     /locus_tag="VNZ06_05680"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HWW20274.1"
                     /translation="MSLDAAAAIAANRFGLGAKPGDLDIIATDPRGWLDAQLKGGPPL
                     LAAPGLKGVGEILSEGFKLTRERREQQREAATSADKLQTVLKRLPQYYRPIYIAEIQA
                     RMRNGVSTQRPFIERLVYFWSNHFAVSVDKLAVTGVAGALEREAIRPHVLGNFYDLLL
                     SVEQHPAMLLYLDNQRSVGPNSVQAQRAAKRERTLGLNENLAREIMELHTLGVDGGYT
                     QADVTSFAKVLTGWSLGLGDVRDPGVAGVFAFRPQLH"
     gene            901..3702
                     /gene="polA"
                     /locus_tag="VNZ06_05685"
     CDS             901..3702
                     /gene="polA"
                     /locus_tag="VNZ06_05685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005095822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HWW20275.1"
                     /translation="MLISSQPDLVLIDGSSYLYRAFFALPPLSTAAGQPTGAIHGVVS
                     MLLKFLREYPPTHIGVVFDAPGRTFRDDLYAEYKAQRQPMPDDLRSQIEPLYQIVDAL
                     GLPLLRIAGVEADDVIGTLACRAAAQGQQVVVSTMDKDMAQFVGERITLINTMSNTVL
                     DRAGVKNKFDVFPEQIVDYLALVGDSSDNIPGVDKVGPKTAAKWLNKYGSLDGLLAHV
                     EEIEGKVGDNLRGARDTIALSRQLATIRIDLEIPAEQSALIRRAPDVARLRDLYKRLE
                     LRTMLTQLEARAPDAVSPAPTTTPNSVADGGRAPIPAVSDQLKATDPHAAALAALPRH
                     YETIVEWEALSRWVDKLAGAELFAFDTETSSLDYMRTEIVGVSFCVEPGHAAYVPLRH
                     DYPGAPDQLDSQRVLDALRPVLEDPSHAKLGQHLKFDMHVLKQAGIEMRGQRFDSMLE
                     SYVLNSTASNHNLDAVSERYLGLSTIRFEDVAGKGAKQLSFNEVPIDRAAEYSAEDAD
                     VTMRLHRVLRARLEQTPSLLRLYDEIEQPLVPVLFRMEHHGVLIDRNMLRVQSGEIAV
                     RLRAVEAEAHREAGAPFNLESPKQLQQILFEKLQLPVLRKTPSGTPSTAEDVLEELAE
                     THALPKHILEYRGLAKLRSTYTDKLPEQINPNTGRVHTSYHQAVAATGRLSSMDPNLQ
                     NIPIRSPEGRRIRQAFIAPPGHRLVAADYSQIELRIMAHLSGDEGLLRAFAEDRDIHQ
                     ATAAEVFGVAPEQVSHDQRRSAKAINFGLIYGMSAFGLARQLGVARSAAQSYVDLYFA
                     RYPGVRRYMDSTREQARRDGYVETVFGRRLYLPDIRSRNRALQQYAERSAINAPMQGT
                     AADIIKRAMVSVDEWCQRPDVPVRLIMQVHDELVLEVREDFVERAEREVAKRMSEAAQ
                     LSVPLKVDAGSGMNWDEAH"
     gene            3772..4302
                     /locus_tag="VNZ06_05690"
     CDS             3772..4302
                     /locus_tag="VNZ06_05690"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWW20276.1"
                     /translation="MRRDLILVLALMLLPSGAALAEDTGFYAGGDVGNSTEHFNGSTF
                     GVNADNGGYKVAVGLRPLPVLAAELDYVGFGRASAGVNFADTYGVSASALAFLPLPIV
                     DVYGRLGVMEWRTDANSPGFSFHRTGSDLTYGVGAGAHWGAFAARLEYERFQVAGAST
                     MQLATAGITYTIGWPF"
     gene            complement(4548..5141)
                     /gene="yihA"
                     /locus_tag="VNZ06_05695"
     CDS             complement(4548..5141)
                     /gene="yihA"
                     /locus_tag="VNZ06_05695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HWW20277.1"
                     /translation="MSIYPGARFLISAAAPTQFPTDFGHEVAFAGRSNSGKSTAINAL
                     TARRALARTSKTPGRTRLINFFELATQQRIVDLPGYGYAEGPPTERDTWKRLIEALGL
                     RSSLQGLFLVVDSRRGLMAGDEQLLAWASDLQKPIHVLLSKSDQLKRSEARVVLLRTE
                     RALQDGASVQLFSARDGTGVEAARNKLNERLLNDALP"
     gene            5184..5855
                     /locus_tag="VNZ06_05700"
     CDS             5184..5855
                     /locus_tag="VNZ06_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWW20278.1"
                     /translation="MSRPQMMSMIRRICLMCVLGIAPIVTPVAMAAGSAEAGATKAAS
                     CVACHGANGNSTNEQWPSLAGQNAAYIKSQLKAFHDKTRLDSLNMMPAMAAPLGEQDM
                     DDLAAYFASQTPSGLEADPSYWQAGQQLYRGGDRNRNIPACMACHGPVGRGNPAAGYP
                     ALRAQHAVYTVKQLTDYASDKRYTRTDKGDSSGGPNSQIMHTIAGALTPEDMRNLASY
                     VQGMR"
     gene            5869..6729
                     /locus_tag="VNZ06_05705"
     CDS             5869..6729
                     /locus_tag="VNZ06_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HWW20279.1"
                     /translation="MRSHLALLLAACLCGCSHESGQAQPAASANTGSASSAGQATSAS
                     PAGSSGTVDLATAAKTQQEGADTGTGNDSTEAALERIAALPASGQLPPGPRVAGKNYQ
                     VLSPAQPTDVPAGKVEVIEMFWYACPHCYALDPTIESWRKNKPAYIEFKRVPVTWNEE
                     HRAHAHLFYTLQALGKLDSLHSKVFDEIHQKGDLLYVPGDEKATMQKQIEFAKANGIS
                     ESDFVARYNDMSVRLKVNQADDLVRRDRIDSVPTFVIDGKYVTDAAMAGDESKLLQLI
                     DDLAASEKHH"
     gene            6787..6863
                     /locus_tag="VNZ06_05710"
     tRNA            6787..6863
                     /locus_tag="VNZ06_05710"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6821..6823,aa:Arg,seq:ccg)
     gene            7087..>8410
                     /locus_tag="VNZ06_05715"
     CDS             7087..>8410
                     /locus_tag="VNZ06_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HWW20280.1"
                     /translation="MPTRTLASGLLCLSAGLFAARAALAEDWLPMSTEELQMKREPEA
                     PAASAIYLYRQVDRSDVENYEHRYYRIKILNEEGLKYANVEIQFVKNRDHIGAISART
                     IRPDGSTVNFDGTVYEKPLMKARQANLFVKSFTLADVQVGSIIEYRYNNRLPDGYIYD
                     SHWILSEDLFTKHARFSLQPYQHFELSYSWPRGLPAGTSAPKLEHGKVGLESSSIPAF
                     VTEEYMPPANEMRYRVDFIYEGNNLSHKDPTTYWKEASKVLYAGIHRFIDQGSAMRNA
                     VAQTVQASDTPEQKLRKLYARAGQIANIDFETDAEREAKDQTAQSIHDVADVWKKGYG
                     KADQITWLYLALVQAAGIEAYPVMVSTRDTYFFDRGFMNSNQLNSNVVLVRLDGKDLF
                     LDPGLPFTPFGMLPWSESSVSGLRLEKGVGTWVFTPQSAAADSRIARQA"
BASE COUNT         1679 a         2612 c         2537 g         1582 t
ORIGIN      
        1 tcatgcagct gcggtcgaaa cgcaaacaca ccggcgaccc ccgggtcccg gacatcgccc
       61 agccccaagg accagccggt aagcactttc gcgaaactgg tgacgtcggc ctgggtgtag
      121 ccgccatcca cgcccagcgt atgcagttcc atgatctcgc gcgccaggtt ctcgttcagt
      181 ccgagcgtac gttcgcgctt ggccgcgcgc tgcgcctgca ccgagttcgg accgaccgaa
      241 cgctgattgt ccagatacag cagcatcgcc gggtgctgct cgacgctgag cagcaggtcg
      301 tagaaattgc cgagcacgtg cggtcggatg gcctcgcgct cgagggcccc ggcgacgccg
      361 gtgaccgcca gcttatcgac cgacaccgcg aagtgattgc tccagaaata gacgagtcgt
      421 tcgatgaacg ggcgctgggt actgaccccg ttgcgcatcc tggcctggat ttccgcgata
      481 tagatcgggc ggtagtactg tggcaggcgt ttgaggactg tctgcagttt gtcagccgag
      541 gttgcggcct cgcgctgctg ttcgcgccgc tcccgggtca gcttgaaccc ttcgctcagg
      601 atctcgccga cacccttcag tccgggggcc gccagcaatg gcggtccgcc tttcaactgt
      661 gcgtccagcc agccgcgagg atcggtggcg atgatgtcca gatcacccgg tttggcgccc
      721 aaaccgaagc gattggcggc gatcgcggct gcggcatcca atgacatagg ccttacctgg
      781 cttcggacgt ggcggcgcaa ttgtgcaccg gttgccactg gaagcgtacc agcagccctg
      841 aggttgacga cacagtccac attgcgggcg catcggccgc tgtcataagg tgcgcgtccg
      901 atgctcattt cctcacaacc ggatctggtg ctgatcgacg gctcgtccta cctgtaccgg
      961 gccttttttg ccttgccgcc actgtcgacg gcggcggggc agcccactgg cgccattcac
     1021 ggcgtggtgt cgatgctgtt gaagttcctg cgtgagtatc cgccaacaca tatcggcgtg
     1081 gtatttgacg caccgggacg cacgttccgc gacgacctgt acgccgagta caaggcccag
     1141 cggcaaccga tgccggacga tctgcgttcg cagatcgaac cgctctacca gattgtcgat
     1201 gccctagggc tgccgctgct gcgcatcgcc ggagtcgaag ccgacgatgt gatcggcacg
     1261 ctggcctgcc gcgctgcggc gcagggacaa caggtcgtcg tgtccaccat ggacaaggac
     1321 atggcgcagt tcgtcggcga gcgcatcacg ctcatcaaca cgatgagcaa tacggtgctc
     1381 gatcgagccg gcgtcaagaa caagttcgat gtgttcccgg aacagatcgt ggactatctg
     1441 gcactggtgg gcgacagcag cgacaacatc cccggtgtcg ataaggtcgg tccgaagacg
     1501 gcagcaaagt ggctcaataa gtatggttcg ctcgacggcc tgctggcgca cgtcgaggag
     1561 atcgaaggca aggtggggga caatctgcgc ggcgcgcgcg acaccatcgc actctcacgc
     1621 cagttggcga cgatccgcat cgatctggaa atcccagcag agcaatccgc gttgatccgt
     1681 cgggctccgg acgttgcgcg gctgcgcgat ctgtataagc gtctggaatt gcgcaccatg
     1741 ctgacgcagc tggaagccag ggcgcccgat gccgtctcgc cagcgcccac cacaacgcca
     1801 aacagtgttg ccgacggcgg ccgcgcaccg atccccgctg tcagcgatca gctcaaggca
     1861 accgatccgc atgccgccgc gctggctgcg ctcccgcgcc attacgagac catcgtggaa
     1921 tgggaggcat tgagtcgatg ggtcgataaa ctcgccggcg ctgaactctt tgccttcgac
     1981 actgagacct ccagtctgga ctacatgcgc accgagattg tcggcgtgtc cttctgtgtc
     2041 gagcccgggc atgcggccta cgtacccctg cgccacgact atccgggcgc tcccgatcag
     2101 ttggacagcc aacgggtgct ggatgccctg cggccggtac tggaggatcc ctcgcatgcc
     2161 aagctcgggc agcatctgaa gttcgacatg catgtgctca agcaagccgg catagaaatg
     2221 cgcggtcagc gtttcgactc gatgctggaa tcctatgtac tcaacagcac tgcgagcaat
     2281 cacaacctgg acgcggtgtc ggagcgctat ctaggcctca gtacgattcg cttcgaagac
     2341 gtcgccggca aaggcgccaa acagctcagc ttcaacgagg tgccgatcga tcgcgcggcc
     2401 gagtactcgg ctgaagatgc cgacgtcacg atgcgtctgc accgggtgct gcgcgcccgg
     2461 ctcgaacaga caccgtcgct gttgcgactg tacgatgaaa tcgagcagcc actggtgccg
     2521 gtgttgtttc gcatggagca ccacggtgtg ctcatcgacc gcaacatgct gagggtgcaa
     2581 agcggcgaaa tcgcggtgcg gctgcgcgcg gtagaagccg aagcgcatcg tgaggccggt
     2641 gcgccgttca acctcgaatc acccaaacag ctgcagcaga tcctgttcga gaaactgcaa
     2701 ttgccggtac tgcgcaaaac cccgagcggc acgccgtcca cggccgaaga cgtgctggaa
     2761 gagctcgccg aaacgcacgc tctgccgaag catatcctcg aatatcgcgg tctggcaaag
     2821 ctgcgttcga cctacaccga caaactgccc gagcagatca acccgaatac cggtcgcgtc
     2881 catacctcct accatcaggc ggttgcggcg accggccgtc tgtcatcgat ggatccgaat
     2941 ctgcagaaca ttccgattcg cagtcccgag ggcagacgca tccgccaggc cttcattgcc
     3001 cctcctggtc atcgactcgt ggcggcggac tactcacaga tcgaacttcg catcatggcg
     3061 cacctctcgg gcgacgaggg actgctgcgc gcgttcgctg aggatcgcga tattcaccag
     3121 gcaacggcag ccgaagtatt cggcgtggca ccggagcagg tatcgcacga tcagcgccgc
     3181 tcggccaagg cgatcaattt cggactcatc tacgggatgt cggccttcgg cctggcacgg
     3241 cagctcggag tggcgcgctc ggctgcccag agctacgtcg atctctattt tgcccgctac
     3301 cccggcgtgc gccgctacat ggattcgacg cgcgagcagg cacgccgtga cggctatgtt
     3361 gaaacagtct tcggtcgtcg gctgtatctg cccgacatcc gttcgcgcaa tcgcgccctg
     3421 caacagtatg ccgaacgcag tgcgatcaat gcaccgatgc agggtaccgc cgcggacatc
     3481 atcaaacgcg cgatggtttc ggtcgatgaa tggtgccaac ggcccgatgt acctgtccgc
     3541 ctgatcatgc aggtgcacga cgaactggtg ctggaagtgc gtgaggattt cgttgagcgg
     3601 gcggagcgcg aggtcgccaa gcgcatgagc gaggccgcgc aactgtcagt acccctgaag
     3661 gtcgatgcgg gcagtggcat gaactgggac gaggcccact aggcgcgtct gtcagccgcg
     3721 ggcgacgtcg gcgcgttgat agcatccaac ccaacaccaa gagggaactc tatgcgtcgc
     3781 gatctcatat tggttctcgc actgatgctg ttgccgagcg gtgccgcact ggccgaggac
     3841 accggtttct atgccggggg cgacgtcgga aattcgaccg agcacttcaa cggcagcacc
     3901 ttcggcgtca atgctgacaa cgggggctac aaagtggccg ttggcttgcg ccccttgccg
     3961 gtgctggctg ccgaactgga ctatgtcggc ttcggacgtg cctccgccgg ggtcaatttt
     4021 gccgatacct acggcgtatc ggcttcagcg ctggcattcc tgccgcttcc gatcgtggac
     4081 gtctatgggc ggctgggtgt catggaatgg cgtacggacg ccaattcgcc gggcttctcc
     4141 ttccatcgta cggggtcgga tctcacctac ggcgttggcg ccggcgcgca ctggggcgct
     4201 ttcgccgcgc ggcttgaata cgagcgtttt caggtggccg gtgccagcac gatgcagctg
     4261 gccaccgctg gaatcactta tacgatcgga tggccgttct agtagcgtcg ctctgagcga
     4321 gataatcgca aacaattgat ccgcacgtta aaaaactttc atccagtttc ggaacttccc
     4381 ggtaccgggc gactcgaact gactgagtgc aagccactcc ccgcttccct ccctaagcgg
     4441 gcacagatcc gacgcgtaga gacccgcgtc gggttggtct ggccccggta gtcaaaacac
     4501 tgccggggca tctttttttg tgcgatagca aatcgatacg gtctcgctca aggtagcgca
     4561 tcgttcagca agcgttcatt cagcttgttt ctggccgctt ccactccagt gccgtcacgc
     4621 gccgagaaca attgcaccga tgcaccgtcc tgcagcgcac gctcggttcg aagcagaacg
     4681 acgcgcgcct ccgagcgctt gagttgatcg gacttggaga gcagtacgtg tatcggtttt
     4741 tgtaagtcgg acgcccaagc gagcaactgc tcgtcgcccg ccatcaaacc gcgccgtgaa
     4801 tccaccacca ggaacagacc ctggagcgag cttcgaaggc cgagcgcctc gatcagacgc
     4861 ttccatgtat cccgctcggt tggaggaccc tcggcgtagc catagccggg cagatccacg
     4921 atgcgttgtt gagtggccaa ttcgaaaaag ttgatcagcc gtgtccgacc gggcgtcttg
     4981 ctcgttcgtg ccagcgcccg acgggcggtg agcgcgttga tcgcggtgga cttgccggaa
     5041 ttggatcgtc ccgcaaaggc cacctcgtgg ccgaaatcgg tcgggaattg cgtgggggcc
     5101 gcggcactga tcagaaagcg cgcgccggga tagatactca tgcgaacagg ccgctggtgt
     5161 acaattctag gatcccttta gttgtgagcc gaccgcaaat gatgtcgatg atccgccgga
     5221 tatgcctgat gtgcgtgctt ggcatagctc ccattgtcac tccagtggcg atggcggccg
     5281 gttcggctga agccggggcc accaaggccg ccagctgcgt agcctgccac ggcgccaacg
     5341 gaaacagcac caatgagcag tggccgagcc ttgccggtca gaacgctgcg tatatcaagt
     5401 cgcagctgaa ggcctttcac gacaagacgc ggctcgattc gctgaacatg atgcctgcga
     5461 tggctgcccc tctgggcgag caggatatgg atgatctggc ggcctacttc gcgtcacaga
     5521 cacccagcgg tctggaggcg gatccttcct actggcaggc cggtcagcag ctctaccggg
     5581 gtggcgatcg caaccgcaat attcctgcct gtatggcatg ccacggtccg gtgggccggg
     5641 gcaatccggc cgccggctat ccggccctgc gcgcgcagca cgcggtttat accgtcaagc
     5701 agctgaccga ctacgcctcg gacaagcgct atacccgcac cgacaagggc gacagcagcg
     5761 gcggaccgaa ttcgcagatc atgcacacca tcgccggcgc actcactccc gaagacatgc
     5821 gcaacctcgc ctcctatgtg cagggaatgc gctgagtaag caacgccgat gagatctcac
     5881 ctggcgctgc tgctggccgc ttgcctttgc ggttgctcac atgaatccgg tcaggcccag
     5941 ccggcggcgt ccgccaatac cggatccgcg agctccgccg ggcaggccac gtccgcgtcc
     6001 ccggccggga gctcggggac ggtggacctg gcaacggccg cgaagacgca gcaggaaggc
     6061 gcggatactg gcacgggaaa cgacagcacc gaagcggctc tggagcgtat cgccgcactg
     6121 ccggcctcgg gccaactgcc acccggacct cgggttgccg gcaagaacta ccaggtactc
     6181 tccccggctc agcctactga cgtgcccgcg ggtaaggtcg aagtcatcga gatgttctgg
     6241 tacgcctgcc cgcactgcta cgcgctcgac ccgaccatcg aaagctggcg caagaacaag
     6301 ccggcctaca tcgaattcaa acgagtaccg gtcacctgga acgaagagca ccgcgcccat
     6361 gcgcaccttt tctacacgct gcaggcctta gggaagctcg attcgctcca cagcaaggtt
     6421 ttcgacgaga tccatcaaaa gggcgacctg ctgtatgtgc cgggcgatga gaaggccacg
     6481 atgcagaagc agatcgagtt tgccaaggcc aatgggatct ccgagtctga cttcgtcgcc
     6541 aggtacaacg acatgagcgt gcgactcaag gtaaaccagg ccgacgatct cgtgcgtcgc
     6601 gacaggatcg atagcgtccc aacgttcgtc atcgacggca aatatgtcac cgatgctgcc
     6661 atggccggtg atgagtcaaa attgcttcag ctgatcgacg atctggctgc cagcgaaaag
     6721 catcactgaa gatcgcccgg caacgattac tcatcgcacg cgaatatccg tagaattgcc
     6781 ggacacgcgc ccgtagctca gctggataga gtggcagcct ccgaagctgc aggtcgtggg
     6841 ttcgaatccc gccgggcgcg ccaattgata gaaaaatgat agaaaaatct gctagtccgc
     6901 cgggcaatct taccgatcga tttcgtccag gatttagtca aatgtatctc ttgtcggcgt
     6961 gtttgtgtga agcgtctctc gacttacccc cgtccgtaaa tggatactga ccggaaataa
     7021 actctcacga tgtccacgtt ggactcgatg gagcgggcag ggtcttaagg acgtagggtg
     7081 gtgctggtgc ctacacgcac tctggcctcc gggctgttgt gtctttcggc gggtctgttc
     7141 gctgcgcgcg ctgcgctggc cgaggactgg cttcctatgt ccacggaaga attgcagatg
     7201 aagcgtgagc ctgaggctcc tgctgcgtcg gcgatctatc tctaccgtca ggtcgatcgc
     7261 agcgacgtcg aaaactacga gcatcgctac taccggatca agattctgaa cgaggagggc
     7321 ctcaaatacg cgaatgtcga gatccagttc gtcaagaacc gcgatcacat cggtgcgatc
     7381 agtgcacgca cgatccgacc ggatggcagc accgtcaact tcgacggcac ggtgtacgag
     7441 aaaccgctga tgaaggcgcg gcaggccaac ctgttcgtca aatccttcac gcttgcggac
     7501 gttcaggtcg gctcgatcat tgaataccgg tataacaatc gcctgcccga cgggtacatc
     7561 tatgactcgc attggatcct gagcgaggat ctgttcacca agcatgccag gttttccttg
     7621 cagccttacc agcacttcga gctgtcgtat agctggccac gcggccttcc tgccggcacc
     7681 tcggccccca agctcgagca cggaaaggtc gggctcgagt cgagcagcat ccctgctttc
     7741 gtgacggagg aatacatgcc tccagcgaac gagatgcgct atcgcgtgga tttcatttac
     7801 gagggaaaca atctgtcgca caaggacccg accacttact ggaaggaggc gagcaaggtc
     7861 ctctacgctg gcatccatcg cttcatcgac cagggctcgg ccatgcgcaa cgccgtcgcg
     7921 cagaccgtcc aggcaagcga tacaccagag cagaagctgc gcaagctcta tgcccgcgcg
     7981 ggacagattg ccaacattga tttcgaaacg gacgctgaac gtgaggccaa ggatcaaacg
     8041 gcccaatcca tccacgatgt cgccgatgta tggaagaaag gctacggcaa ggccgatcag
     8101 atcacctggc tgtatctcgc attggtgcag gcagccggca tcgaagccta cccggtgatg
     8161 gtatccaccc gcgataccta tttcttcgac cggggcttca tgaactccaa tcagctcaac
     8221 tccaacgtcg tactggtcag actcgacggc aaggatctgt ttctggaccc gggtctgcca
     8281 tttaccccgt tcggcatgct gccgtggagt gaaagttcgg tgtccggtct acgcctcgaa
     8341 aaaggcgtcg gcacctgggt gtttacccca caatcggccg cagccgattc acgcatcgcg
     8401 cgccaggcgg
//
LOCUS       DATMRM010000195         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3510504, whole genome shotgun sequence.
ACCESSION   DATMRM010000195 DATMRM010000000
VERSION     DATMRM010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3510504"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VNZ06_05720"
     CDS             <1..481
                     /locus_tag="VNZ06_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HWW20281.1"
                     /translation="PEVNARWHDDSLEIFDPIDIGVGTAVEGKGLIVPVVRGVQTRNL
                     KEIASELGRLVDAAREQRLTPNDVRGGTFTLSNHGVSGSLLAAPIVINQPQVAILGVG
                     KLEKRVAVLEQAGEDKIAVRPRCYISLTIDHRALDGDRANRFLQVLVERLEGWPQQN"
     gene            492..1790
                     /gene="paaK"
                     /locus_tag="VNZ06_05725"
     CDS             492..1790
                     /gene="paaK"
                     /locus_tag="VNZ06_05725"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HWW20282.1"
                     /translation="MLDAIEGASIDELRALQLKRLQWSLSQAYRQVEHYRGSFDRAGI
                     HPEQIRRLEDLQRVPFLTKQDFRDHYPFGLLAVPRDQIIRLHASSGTTGQATVVGYTR
                     HDIDTWAGLVARSIHAAGGRRGDRIHVAYGYGLFTGGLGAHYGAERLGCAVIPMSGGQ
                     TEKQVQLIRDLGPDLIMVTPSYLLAIGEEFVRQGADPAETSLRAGILGAEPWSEAMRQ
                     QIESRFHIDALDIYGLSEVMGPGVASECVETKDGPVIWEDHFYPEIIDPVTGAVLPDG
                     EEGELVLTSLSKEALPVIRYRTRDRTRLLRPTSRSMRRLARIAGRTDDMLIIRGVNVF
                     PSQIEALILEQPVLAPHYYLELWREGHLDAITVHVELREVLGDVEALRASSRHELIRR
                     VKSYVGISARVTVHDAGTITRSAGKAKRVIDRRPGVASDY"
     gene            complement(1811..>2469)
                     /locus_tag="VNZ06_05730"
     CDS             complement(1811..>2469)
                     /locus_tag="VNZ06_05730"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWW20283.1"
                     /translation="ADASRPTTDRSRDTDRKPAETLEFAHIKPGDNVADFAAGGGYFS
                     RLFADVVGPKGHVYTIQPDEEAKYTAKATAELQEFSKTHDSLSVVTGTAMNTLKFDKP
                     LDVFWISQAYHDLKDKFFGPVDTAAFNKAVFAALKPGGTYIVLDHVAAAGAPADVTET
                     LHRIDPALVKREVEAAGFKFERESKLLENPSDPHTAGVFDKSIRGKTDQFIYKFRKPK
                     "
BASE COUNT          449 a          729 c          826 g          465 t
ORIGIN      
        1 tcccgaagtc aatgcgcgct ggcacgacga ctcgctggag atcttcgatc cgatcgatat
       61 cggagtcggg accgcggtgg aaggcaaagg cctgatcgtg ccggtcgtgc gtggcgtaca
      121 gacccgcaat ctcaaggaga tcgcgagcga actcggccgg ctggtggatg ccgcgcgcga
      181 gcagcggctg acgccgaacg atgtgcgcgg cgggaccttc accctttcca atcatggcgt
      241 gagcggcagc ctgctggcgg caccgatcgt gatcaatcag ccccaggtcg cgatcctggg
      301 cgtgggcaaa ctggaaaaac gcgttgccgt gctggagcag gccggtgagg ataaaatcgc
      361 tgttcgaccg cgctgctata tcagtctgac gatcgatcac cgggcgctcg acggggatcg
      421 tgcgaaccgc ttcctgcagg tactggtcga acggctggag gggtggccgc agcaaaactg
      481 aaggtattca catgctcgat gcgatagaag gggccagcat cgatgagctg cgggcactgc
      541 agctcaagcg cctgcaatgg agcctgtcgc aggcttaccg ccaggtggag cactaccgcg
      601 gcagctttga tcgcgcgggc attcatcccg agcagatccg ccggctcgag gatctgcagc
      661 gcgtgccgtt cctcaccaag caggatttcc gcgatcacta cccgttcggg ctgctggcgg
      721 tgccgcgtga tcagatcatc cgcctgcacg cttccagcgg aaccaccggc caggccaccg
      781 tcgtcggcta tacgcgccac gatatcgata cctgggcggg cctggtggcg cgctccattc
      841 atgccgcggg tggccgtcgg ggcgaccgga tccatgtggc gtacggctac ggactgttca
      901 ccggtggtct gggcgcgcac tacggtgcgg agcgtctggg ctgcgcggtc attccgatgt
      961 ccggcgggca gaccgagaag caggtgcagc tgatccgcga tctcgggccc gatctgatca
     1021 tggtgacacc gtcctatctg cttgcgatcg gcgaagagtt cgtgcggcag ggcgcggacc
     1081 cggccgagac ctcgctgcgt gccgggatct tgggcgctga gccgtggagc gaggcgatgc
     1141 ggcaacagat cgagtcgcga ttccacattg acgcgctcga catctatgga ttgtcagagg
     1201 tgatggggcc gggcgttgcc agcgagtgtg ttgagaccaa ggacggcccg gtgatctggg
     1261 aggatcactt ctacccggaa atcattgatc cggtgaccgg cgccgtgttg cccgatggcg
     1321 aagagggtga actggtgttg acctcactgt cgaaagaagc cttgccggtc attcgctatc
     1381 gcacgcgcga tcgtacgcga ctgctgcggc cgaccagccg ttcgatgcgc cgcctcgcgc
     1441 gcatcgcggg ccgcaccgac gacatgctga tcatccgcgg cgtgaatgtg tttccgtcgc
     1501 agatcgaagc gctgattctc gaacagccgg tgctcgcgcc acactactat ctggagctct
     1561 ggcgcgaagg gcacctcgac gcgatcaccg tacacgtcga gctgcgagaa gtgctgggcg
     1621 atgtggaggc gctgcgcgcc tcgagccggc acgaactgat ccggcgggtg aaatcctatg
     1681 tgggtatcag cgcacgcgtg accgtgcatg atgcaggaac aatcacgcgc tcagccggga
     1741 aggctaagcg cgtgatcgac cgtcggccag gtgtagcgtc tgactactag gggagaggct
     1801 acgggtctgg ctacttaggt ttgcggaact tgtagatgaa ctgatcggtt ttgccgcgga
     1861 tcgacttgtc gaagacaccg gccgtgtgag gatcggaagg attctcaagg agcttgcttt
     1921 cccgttcgaa cttgaagcca gcggcctcga cctcacgctt cacgagcgcc gggtcaatgc
     1981 gatgcagggt ctcggtcaca tccgccggcg cgccggcggc ggcgacatga tccagcacga
     2041 tataggtacc accgggcttc agcgccgcga acaccgcctt gttgaacgcg gcggtatcca
     2101 cgggaccgaa gaacttgtcc ttgagatcgt gataggcctg cgagatccag aacacatcga
     2161 gcggcttgtc gaacttcagt gtgttcatcg ccgtgcccgt gaccacggac aggctgtcgt
     2221 gagtcttgga aaactcctga agttcggcgg tcgccttggc ggtgtatttg gcttcctcgt
     2281 ccggctggat cgtgtagacg tgacctttcg gtccgaccac gtccgcgaac agccgtgaga
     2341 aataaccgcc gccggcggca aaatccgcaa cgttgtcacc cggcttgatg tgagcaaatt
     2401 ccagcgtttc ggctggtttg cgatcggtat cgcgcgaacg gtcggtcgtc ggtcggctgg
     2461 catcggcca
//
LOCUS       DATMRM010000196         4138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3203564, whole genome shotgun sequence.
ACCESSION   DATMRM010000196 DATMRM010000000
VERSION     DATMRM010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3203564"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /gene="fadE"
                     /locus_tag="VNZ06_05735"
     CDS             complement(<1..533)
                     /gene="fadE"
                     /locus_tag="VNZ06_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="functions in fatty acid oxidation; converts
                     acyl-CoA and FAD to FADH2 and delta2-enoyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWW20284.1"
                     /translation="MIGAVFTVLFVVAVLYLAYRRLPLFFFTISFTVLMVAYWLLGHP
                     SGVWQGLLWLLLILFWLLNLRPLRIALISRPFMRRYRRLLPPMSQTERDALEAGTVWW
                     EGELFTGNPDWRKLRAAPAPRLTDEEQAFLDGPVETLCAMLDDFDITHRRADLPPEVW
                     DYIKSQRFFAMIIPKRYG"
     gene            complement(610..1593)
                     /locus_tag="VNZ06_05740"
     CDS             complement(610..1593)
                     /locus_tag="VNZ06_05740"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HWW20285.1"
                     /translation="MKQPLNVAITGAAGQIGYALAFRVASGAMLGAEQPINLHLLEIT
                     PALQTLNGVVMELNDCAFPTLNRIVATDEATLAFRDCHVGLLVGARPRGPGMERKDLL
                     LANAQIFSAQGKALNEVADRKIKVLVVGNPANTNALIASRNAPGLDRRCFTAMTRLDH
                     NRALSQLAEKTGTHVNDIRHMSIWGNHSATQYPDLSHALVKGKPAKGLVDQPWIEQHF
                     IPTVQQRGAAVIKARGSSSAASAASAALDHVRTWVQGTADGDWTSMGIPSEGSYGVKE
                     GLIYSYPVTCKNGDYQIVQALSIDEFSRKRMDITENELREERDGVKDLLGG"
     gene            1715..1969
                     /locus_tag="VNZ06_05745"
     CDS             1715..1969
                     /locus_tag="VNZ06_05745"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HWW20286.1"
                     /translation="MTEPQIAGRAPIAVDVEAGKTYYWCSCGGSVNQPFCSGAHRGSE
                     FEPIAYVADRAGKLYFCTCKRSGKKPLCDGTHKTLSPPAT"
     gene            2092..2793
                     /locus_tag="VNZ06_05750"
     CDS             2092..2793
                     /locus_tag="VNZ06_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HWW20287.1"
                     /translation="MRDHLKWFAPLLLLVSVCHADGIGGDVGISSDEVFRGLSQSDHQ
                     WSPQLDLHYSFSGWYAGLTAVGVRRGQYESVRAGLIAYLGYQYRLNDDWAMSLAARHY
                     DYPGYPYRHYYDYEEGALSVSWRELIVATVMASPNVYFADFQGRAARGPAYTYEIGGR
                     LPLAYGFCANAGVGYYNLDRQIGTGYAYWSTGISKQWRSVNFDLRYVGTDETATRRFE
                     QFAENRLVFSALYLF"
     gene            2896..3510
                     /locus_tag="VNZ06_05755"
     CDS             2896..3510
                     /locus_tag="VNZ06_05755"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWW20288.1"
                     /translation="MSYSANTKVAMPARDDRLDRELLRSVANGDARALEHLYLEYHRR
                     LLQFLSRLSSRREALEEAINDTFWIVWQKAKEFRGGSRVSTWIMGIAWRCAMKALRRN
                     SDSSAEAFANSMPVETASAESLVVEERGEWLERGLATLPMEQRATLELAYYVGHSCEE
                     IAIIMACPVNTVKARMFQARIKLRNLLPKLGGFHDTMISGGGQL"
     gene            3510..>4138
                     /locus_tag="VNZ06_05760"
     CDS             3510..>4138
                     /locus_tag="VNZ06_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWW20289.1"
                     /translation="MNDTSMHRHTWDQIPWMVNGTLPPAERDALQAHLTGCADCRAEL
                     EFQRRLAASLEAGSVCEIDSQESWKQLRARIEAVGRSEVTRHGQRRSRGSLAGEWIPW
                     LVAAMVVQAIGLGALGTVIWSKPGASVTSGSTYRTLSGPEPPVGGATLRVVFAPDMSV
                     GNMQALLSAAGLQVQSGPSSAGVWSLEPARDSNRSATQSALRELRDSPAV"
BASE COUNT          744 a         1262 c         1331 g          801 t
ORIGIN      
        1 ccgtagcgct tcggaatgat catcgcgaag aagcgttgcg atttgatgta gtcccagact
       61 tcgggcggca ggtccgcgcg ccgatgagtg atatcgaagt catccaacat ggcgcacagc
      121 gtctcgaccg gtccgtcgag aaaggcctgc tcctcgtcag tcaggcgggg agccggcgcc
      181 gcgcgcagct tgcgccagtc ggggttgccg gtgaacagct ctccctccca ccagacggtg
      241 ccggcctcga gcgcatcacg ctcggtctgc gacatcgggg gcagcaatct tcgatagcgg
      301 cgcatgaacg ggcggctgat cagcgcgatg cgcagcggcc gcaggttcag cagccagaac
      361 aggatcagca gcagccatag tagcccctgc cacacccccg acgggtgtcc gagcagccaa
      421 taggccacca tcaataccgt gaaagagatc gtgaaaaaga atagtggcag gcggcggtat
      481 gcgagataca gcaccgcgac cacgaacaac acggtgaaca ccgcgccgat catgctccgc
      541 ccttgaagta agccatgcgg ccgctactta aaaccatcgc ggcgcgccta tgcaagcgga
      601 aggcgttttc tagccgccga gcaggtcctt gacgccgtcg cgctcctcgc gcagctcgtt
      661 ttcggtgatg tccattcgtt tacggctgaa ctcatcgata ctcaaggcct ggacgatctg
      721 atagtccccg ttcttgcagg tcaccgggta ggagtagatc agcccctctt taacgccgta
      781 gctgccttcc gacgggatcc ccatgctggt ccagtccccg tcggcggtac cctgcaccca
      841 ggtgcgcacg tgatcgagcg ccgccgaggc agccgatgcc gccgaggagg agccgcgcgc
      901 cttgatcacg gcagcgccgc gctgctgtac cgtcggtatg aagtgctgct cgatccacgg
      961 ctgatcgacc agccccttcg ccggtttgcc tttgacgagg gcgtggctaa ggtccgggta
     1021 ctgcgtagcg gagtgatttc cccagatgct catgtgtcgg atgtcgttga cgtgcgtacc
     1081 ggtcttttcc gccaattgtg atagcgcacg gttgtgatcc aggcgggtca tggccgtgaa
     1141 gcagcgtcga tccagcccgg gagcattgcg gctggcaatc agcgcgttcg tgttcgcagg
     1201 gttgcccacc accagaacct tgatcttgcg atcggcgacc tcgttgagcg ctttgccctg
     1261 tgccgagaag atctgggcat tggccagcag cagatccttg cgctccatgc ccggaccgcg
     1321 cggccgtgca ccgaccagca ggcccacgtg gcagtcgcgg aaggcgagcg tcgcctcgtc
     1381 ggtggcgacg atcctgttga gcgtcggaaa tgcgcaatcg ttcagctcca tgaccacgcc
     1441 gttgagcgtt tgcaacgccg gagtaatctc cagcagatgc aggttgatcg gctgctcggc
     1501 tcccagcatc gcaccggagg cgacgcggaa ggccaatgca tagccgatct ggccggcggc
     1561 accggtgatc gcgacgttca gaggctgctt catagaaact ccgctcccag accgacgcgc
     1621 attctagccg gttacacaaa agcggttgtc actgtatgaa tcatcagggt taccatgcgt
     1681 cttcagcacc acaggaactg aaggagaaag caacgtgacg gaaccgcaga tcgctggccg
     1741 cgcgcccatc gccgtcgacg tcgaggcagg caagacctac tactggtgca gctgtggcgg
     1801 ctcggtgaat cagccgttct gcagcggtgc ccaccggggc agtgagtttg agccgatcgc
     1861 atacgtcgcc gatcgcgccg gcaagctcta tttctgtacc tgcaagcgct ccggcaagaa
     1921 accactgtgt gacggcaccc acaagacact gtcgccgccg gcgacgtgag agcctatcgc
     1981 gagtcaggct atgatccagg gcgcgccgac gcccggtatg gcgcagtaca ccgtttgggc
     2041 catcgagcgt ttgtctgatt tccttcggcg tttatctctt ccggtaacaa catgcgtgac
     2101 catctgaagt ggtttgctcc gctgttgtta ctggtatccg tgtgtcacgc ggatgggatc
     2161 ggtggtgacg tcggcatttc gagcgacgag gtgttccgtg gcctgtcgca gagcgatcac
     2221 cagtggtccc cccagttgga tctgcactac agcttttcgg gctggtacgc cggcctgacg
     2281 gcggtcgggg tgcgtcgcgg acagtacgag tcggtccgcg ccgggctgat cgcctatctg
     2341 ggctatcaat accggctgaa tgacgattgg gcgatgagcc ttgctgcacg gcactacgac
     2401 taccccggct acccgtaccg tcactactac gactatgagg agggggcgct gagtgtgagc
     2461 tggcgcgagc tgattgtcgc cactgtgatg gcctctccta acgtgtactt cgctgacttc
     2521 cagggtcgcg ctgcccgcgg gcctgcctac acctacgaga tcgggggtcg tctgcccctg
     2581 gcctacggtt tttgtgccaa cgccggtgtg ggctactaca atctggaccg ccagatcggc
     2641 actggctatg cctactggag taccggcata agtaaacaat ggcgctcggt gaatttcgac
     2701 ctgcgttatg tcggcaccga tgagaccgcc acgcgccggt tcgagcaatt cgccgagaac
     2761 cggctcgtat tcagtgcctt gtacctgttc taatcggcac tgggcgaagg cgatcgcaac
     2821 tccctgcggc tacaaccttt tatcggccgg gcggcaccta cattcgaggg gcatgatatc
     2881 cacgcggttt tgacgatgtc gtactccgcc aacaccaagg ttgctatgcc ggcacgcgat
     2941 gatcgactgg atcgagagct gttgcgatca gtcgccaacg gcgatgcgcg cgcgctcgag
     3001 cacctgtatc tcgagtatca ccggcgactg ttgcaatttc tgtctcgcct gagctcgcgt
     3061 cgcgaggcgc tcgaagaggc gatcaacgac acgttctgga tcgtgtggca gaaggcaaag
     3121 gagttccgcg gcgggtcgcg cgtgtccacc tggatcatgg gcattgcctg gcgctgcgcg
     3181 atgaaagctc tgcgtcgcaa cagcgattcc tcggccgagg catttgccaa ttcgatgccc
     3241 gtggagacgg ccagtgccga gtcgctggtg gttgaggagc gcggcgagtg gttggagcgg
     3301 ggtctggcga cgctgccgat ggagcagcgg gcgaccctgg agctggcgta ttacgtgggt
     3361 cattcgtgcg aggagatagc catcatcatg gcctgtccgg tcaatacggt gaaagcgcgc
     3421 atgtttcagg cgcgcatcaa actgcgaaac ctgctgccca agctgggtgg ctttcatgac
     3481 acgatgatct cgggtggggg tcaactgtga tgaatgacac ctcgatgcac cgtcatacct
     3541 gggatcagat tccctggatg gttaacggca cgctgccgcc ggccgagcgt gatgcgctcc
     3601 aggcgcacct gacaggctgc gcggactgtc gcgccgaatt ggagttccag cggcgccttg
     3661 ccgcctcgct cgaggcgggc agtgtgtgcg agatcgactc tcaggagtca tggaagcagt
     3721 tgcgcgcgcg tatcgaggcg gtcggtcgct ccgaggtgac gcgccacggc cagcgtcgca
     3781 gccgcggctc gctcgcaggg gagtggatcc cctggctcgt ggctgccatg gtggttcagg
     3841 ccatcggact gggagccctc ggcacggtga tctggtccaa gcccggcgcg tcggtgacca
     3901 gcgggagtac ctatcgaacc ctgtcaggac cggagccgcc ggttggcggc gcgaccctac
     3961 gtgtggtgtt cgcccctgac atgagcgttg gcaatatgca ggcactgctt tcagccgccg
     4021 gactgcaggt acaatcgggt ccgagcagcg ccggagtctg gtcgctggag ccggcgcgcg
     4081 attccaaccg ctcggcgacg caatcggcac tgcgcgagct gcgcgacagc cccgcggt
//
LOCUS       DATMRM010000197         3180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3829013, whole genome shotgun sequence.
ACCESSION   DATMRM010000197 DATMRM010000000
VERSION     DATMRM010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3829013"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..759
                     /gene="maiA"
                     /locus_tag="VNZ06_05765"
     CDS             118..759
                     /gene="maiA"
                     /locus_tag="VNZ06_05765"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013786010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HWW20290.1"
                     /translation="MQLHSFFNSSTSYRVRIALALKGLAYDTLPVNIRVGEHRAADYV
                     ANVNPSASVPALIDGVFKLGQSLAIIDYLDQTHPEPRLIPLEPAVRARVLELSTLIAC
                     DIHPVNNVRVLRYLETELKVTPQQKTAWYRHWIAQGMAAVERLLERSNEGPWCFGAQP
                     TLADVSLVPQIANALRMDCDLGACPRSLAVYEHASKHPAFAAAEPQRQPDYVA"
     gene            870..2081
                     /locus_tag="VNZ06_05770"
     CDS             870..2081
                     /locus_tag="VNZ06_05770"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: protein motif:HMM:NF006021.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HWW20291.1"
                     /translation="MSETNKARRVLVIGGGIGGLAAALALARQGIRVQLLEQAPEIQE
                     IGAGIQLAANAFNALDALGVGEAARGRAVFTDYLSLRDALDAKEIVRIDVGAPYRARF
                     GNPYAVIHRADIHLSILEAVKDHPLIQFRTNTQVCALDQDANGVTVTDQHGERYSADA
                     VIGCDGVKSAIRRALIGDAHRVTGHVVYRAVVDVENMPEDLRINAPVVWAGPHCHLVH
                     YPLRGGRQYNLVVTFHSREKEEWGVREGSKQEVLSYFEGIHPLPHQMLDRPTSWKRWA
                     TADRDPVERWSEGRATVLGDAAHPMTQYLAQGACQALEDAVTLGAAVAATEGDFEAAF
                     KLYESVRIPRTARVLYSAREMGRIYHAKGVERLVRNSLWVGRTQEQFYDALAWLHGWR
                     AEDCLNGAAQG"
     gene            complement(2105..2440)
                     /locus_tag="VNZ06_05775"
     CDS             complement(2105..2440)
                     /locus_tag="VNZ06_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20292.1"
                     /translation="MYLPVLLELAPSRVRRAQPRKQIARGYMDASVRMTHMGPGCTAG
                     LCEKGVTFSLDYLQFFVGDDAHAKKKVGALIEQPGFFGIDLGLLEAGGRLVQIPGGPL
                     PVLNLVKLG"
     gene            complement(2597..>3180)
                     /locus_tag="VNZ06_05780"
     CDS             complement(2597..>3180)
                     /locus_tag="VNZ06_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HWW20293.1"
                     /translation="WAGFSFRVQDGFGNQAGNFRGNRQFSVSAQYTLTNFAAYGVYEE
                     IHDANGHFSDLYTASREYMAGVTYQLGGLKFYTGYQQLVSSGQDTVATSFNPFAATRS
                     QQVWVGASYQATPALALQAGWYHGNVNHGGGEGNLGAIATTYNLSKRTFLYLTLGAMF
                     NGKSSAFPVETADSQPLPGHNQQGGYFGIMHFF"
BASE COUNT          602 a          930 c         1035 g          613 t
ORIGIN      
        1 gaaggcgttg gctcagtgaa gacgggcgac acgatgaaag tcggcgtcga gcgtctgggc
       61 gaagtgacgg tacgcgtggc ctgacatacg taatctgcat tggacggagg tctactcgtg
      121 caactccata gcttcttcaa cagctcgacg tcgtaccgcg tgcgcattgc gctcgcgctc
      181 aaaggtctcg cgtacgacac gctgcccgtc aatatccgtg tcggcgagca tcgcgcagcg
      241 gattacgtgg cgaacgtgaa tccatccgca tcggtgcccg cgttgatcga cggcgttttc
      301 aagctcggcc agtcactcgc gatcatcgac tatctcgacc agacccatcc cgagccgcgc
      361 ctgattccgc tcgaaccagc agtgcgggcg cgcgtactcg aactttcgac gctgattgcc
      421 tgcgacatcc atcccgtcaa caacgtgcgc gtgctgcgct atctggaaac ggaactgaag
      481 gtgacgccgc agcaaaaaac cgcgtggtat cgacactgga tcgctcaagg gatggcggcc
      541 gtcgagcgcc tgctcgaacg cagcaacgaa ggcccgtggt gcttcggtgc gcaaccgacg
      601 ctcgctgacg tgtcccttgt gccgcagatc gccaacgcgc tgcgcatgga ctgcgatctg
      661 ggcgcctgtc cgcgcagcct cgctgtgtat gaacacgctt cgaaacatcc ggcattcgct
      721 gcagcagagc cgcagcgcca gcccgattac gttgcttgat tgcatgggac gggagcgctt
      781 ttgcgtggga ccggagtcag tcctgaagca ctaactccgg atcgacataa gcgttaaagc
      841 gctcactccc gtcgacaaag gagacagtca tgagtgagac aaacaaggcg cgccgcgtgc
      901 tcgtaatcgg cggcggtatt ggtggactgg ctgctgcgct ggcgctcgca cggcagggca
      961 ttcgcgtgca gctgctcgag caggctcccg agatccagga gataggcgcg ggcatccagc
     1021 tggccgcgaa tgcgttcaac gcgctcgatg cgttgggtgt cggcgaggcg gcgcgcggcc
     1081 gggccgtgtt caccgactac ctgagcctga gggacgcgct cgacgcgaag gaaatcgtgc
     1141 gcatcgacgt tggcgcgcca tatcgcgcgc gctttggcaa tccgtatgcg gtgattcatc
     1201 gtgcggatat ccatctgtcg attctcgaag ccgtcaagga tcatccgctg atccagttcc
     1261 gcacgaacac gcaggtctgc gccctggatc aggatgccaa cggcgtgacc gtcaccgatc
     1321 agcatggcga gcgctacagc gcggacgcgg tgattggttg cgatggcgtg aaatcagcga
     1381 tacgcagggc gctgatcggt gacgcgcatc gcgtgacggg gcatgttgtg tatcgcgcag
     1441 tcgtcgatgt cgagaacatg cctgaggact tgcgaatcaa tgcacctgtc gtgtgggcgg
     1501 gtccgcattg tcaccttgtg cattatccgc tgcgcggcgg ccggcagtac aacctggtgg
     1561 tcacgttcca tagccgcgaa aaggaggaat ggggcgtgcg cgaaggcagc aagcaggaag
     1621 tgctgtcgta cttcgagggc atccatccgc tgccgcacca gatgctcgac cggccgactt
     1681 catggaagcg ctgggccacg gcggatcgcg atcccgtgga acgctggagc gaggggcgcg
     1741 ccaccgtgct cggcgacgcg gcgcacccaa tgactcagta cctcgcgcag ggtgcctgtc
     1801 aggcgcttga agacgcagtc acgctcggcg cggccgttgc cgcgactgaa ggcgatttcg
     1861 aagcggcgtt caagctgtac gagtcggtgc gcattccgcg cacggcgcgc gtgctctact
     1921 cggcgcgcga aatgggccgc atctatcatg cgaagggcgt cgagcgtctc gtgcgcaatt
     1981 cgctttgggt cggccgcacg caggagcagt tctacgacgc gttggcgtgg ttgcatggct
     2041 ggcgcgccga agactgtctg aatggcgccg cccagggata acggcacggg tcgcctttgg
     2101 ctggttatcc caacttgacg agattgagca ccggcagcgg accgcccggg atttgaacca
     2161 gccggccccc ggcctcgagc aaacccaggt cgatgccgaa gaagccgggc tgctcgatca
     2221 gtgcgcccac tttttttttc gcatgtgcgt cgtctcccac gaagaactgg agataatcga
     2281 gtgagaacgt gacgcctttc tcgcatagcc cggctgtgca tccaggcccc atgtgcgtca
     2341 ttcgcacgct ggcgtccatg tagccacgag caatctgttt gcgcggctgt gcccgtctta
     2401 cccgagatgg agcaagctcg agcaggacgg gcaggtacac atgttctttt gcgcgctcgc
     2461 gtccagggga aaataacagc cccgggacgc gaaccttacc cggattacgg gaaggggatg
     2521 ttgcaacgct gcgagcgcgc ttgcgcgcgg ctgtaggcgc tggcgtacat gtgtctgtac
     2581 gctaccgtgg catcggttag aagaagtgca tgatgccgaa gtagccgcct tgctggttat
     2641 ggcccggcag aggctgggaa tcggccgtct caacagggaa agctgagctt ttgccattga
     2701 acatcgctcc gagggtcagg tagaggaatg tccgttttga caggttgtag gtggtggcga
     2761 tggcaccgag atttccctct ccgccgccat gattgacgtt tccgtgatac cagccggcct
     2821 gcaaggcgag ggcaggggta gcctgatacg aggcgcctac ccagacctgc tggctgcgtg
     2881 tggcggcgaa aggattaaac gaagtggcga ccgtgtcctg tccggatgag accagttgct
     2941 gatagccggt gtagaacttg agtccgccta actggtatgt gacacccgcc atatattctc
     3001 gtgacgccgt atacaggtct gaaaaatgcc cgttggcgtc gtggatttct tcataaactc
     3061 cgtacgcggc gaagttggtc agtgtgtact gtgcgctcac actgaactgg cggttgccac
     3121 ggaagttgcc ggcctggttg ccgaatccgt cctggacccg gaaggagaag ccggcccagg
//
LOCUS       DATMRM010000198         5616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2283859, whole genome shotgun sequence.
ACCESSION   DATMRM010000198 DATMRM010000000
VERSION     DATMRM010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2283859"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VNZ06_05785"
     CDS             <1..281
                     /locus_tag="VNZ06_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWW20294.1"
                     /translation="KTANVVLNVAYGEPTMAVDTHVFRVANRTGLARGENPRVVEDEL
                     VRVIPAQFMNHAHHWLILHGRYVCKARVPACPQCAIARWCEYQPKTSL"
     gene            complement(351..1805)
                     /locus_tag="VNZ06_05790"
     CDS             complement(351..1805)
                     /locus_tag="VNZ06_05790"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HWW20295.1"
                     /translation="MNNANAPSALGASGACGRRAVNLAVGVALLGALSAVSGVASAQS
                     TVGPVMAPSDDSLTYKGITLYGIIDVGFQYETHGAPFSDYFMASSADIVQKDSNHSAT
                     GLTSNNLSQSRIGLAVLEPLGVGDWYGIGRVETYFNPSSGDISDALKSLAQNNGRAVT
                     AQTTNLDSSIAGELFEQAYLGLSSATFGAVTFGRQNTLLADGIAKYDPNAASQAFSII
                     GLSGTTAGGGDTQDRRLDDSVKYSEAIGPVHIGILYKFSQSFGAANTAEQFDVGFDYA
                     GLSVDALYAHVRDAVAASALSAAQEAIAVAAPNYLSTSNSVSGTISDNQSFTILGLYN
                     LGFIPVKVYAGYEHIQFGNPKNPLAIGFDDIGGYKLGAVNNTAFPTEKVLQVAWAGVK
                     YTWGKKLDLTAAWYGYDQNSYGVGKGIAGCSSTVAGTCSGTETVLSVDADYRWTKRFD
                     TYAGVMYSSVSDGLANGYIKTTNADPTIGFRFRF"
     gene            complement(1960..2811)
                     /gene="pstA"
                     /locus_tag="VNZ06_05795"
     CDS             complement(1960..2811)
                     /gene="pstA"
                     /locus_tag="VNZ06_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWW20296.1"
                     /translation="MSIRTRRIKASIALGASGVATAIGLAFLAAILWTLVARGFGGLS
                     WTVFTQTTPAPGSSGGLLNAIYGSLMMTLLGVLIGGPIGILAATYLSEYGRTARLSTV
                     IRFINDVLLSAPSIVIGLFVYTLVVVRMGHFSAIAGVVALGIIVIPVTVRTAEDMLRL
                     IPQNMRHASAALGAQPWRTITSIIYPAARSGLLTGVLLALARVSGETAPLLFTALNNQ
                     FWNANLNQPIANLPVVIFQFATSPYPNWQSLAWAGALLITLFVLLLSVLARLISSRFG
                     SRESRIE"
     gene            complement(2808..3773)
                     /gene="pstC"
                     /locus_tag="VNZ06_05800"
     CDS             complement(2808..3773)
                     /gene="pstC"
                     /locus_tag="VNZ06_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWW20297.1"
                     /translation="MSATTLPFHAQLKREAGAEVVFRWLTIGSAALVLILLAGVMVAL
                     CDGGWPAIKTFQFAFLTREVWNPVTEQFGALAALYGTLITSLVAMLIAVPLSFGIAVF
                     LTETCPLWLRGPIATCVELLAAVPSIVYGIWGLFVLAPILQHTVQPFLIDTLGDLPLL
                     GKLFRGPPFGIGLFTAGIVLAVMVLPFITSVMREVFLATPRSLKEAAYGLGATQWEVI
                     WDVVLPNSRAGVIGGIMLGLGRALGETMAVTFVVGNAHRISTSLLAPGTTISASIANE
                     FTEAVGTLYTSSLIALGLLLFIITFTIIAAARLLLLQIERRGARI"
     gene            complement(3819..4916)
                     /gene="pstS"
                     /locus_tag="VNZ06_05805"
     CDS             complement(3819..4916)
                     /gene="pstS"
                     /locus_tag="VNZ06_05805"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF008171.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HWW20298.1"
                     /translation="MNFFVPSTPGSWSGRIWSGRILMCAGIAAVALSGAFVGHAQADD
                     ISGAGATFPYPIYAKWAEAYKAQTGVGLNYQSIGSGGGIKQIEAGTVTFGASDKPLEP
                     ADLKQNGLVQWPMVIGGVVPVVNIPGIKPGDVQLDGATLAAIFLGEITRWSDPAIKKL
                     NPSLNLPKLDIAPVTRSDGSGTNFLFTDYLSKVSPSFKQKVGANTSVQWPSGIGAKGN
                     EGVANQTTQTSGAIGYVEYAYVKQTHMTYVRMANHDGKYVAPEAKAFQAAASSADWAK
                     AEGYYLILTDQAGAASWPITGASFILMHADSKDANTSAALKFFDWAYKSGTKMAEDLD
                     YVPIPESVSKLVEKTWAASLKVGGKAAWPAK"
     gene            5179..>5616
                     /locus_tag="VNZ06_05810"
     CDS             5179..>5616
                     /locus_tag="VNZ06_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWW20299.1"
                     /translation="MRLLIVEDDPKLADFLARGLRAERFAVDVAPDGQQGQSFLESYA
                     YDLLILDLMLPRLSGTALLQWLRQRRAALPVLVLTARDATEDKVKHFEAGADDYLTKP
                     FDFAELVVRVRALLRRSPVERADVLRIADLELNRLTQQVRRGGQ"
BASE COUNT         1127 a         1744 c         1701 g         1044 t
ORIGIN      
        1 gcaagaccgc caacgtggtg ctcaatgtcg cgtacggcga gcccacgatg gcggtcgata
       61 cgcacgtatt tcgagtcgcc aatcgcaccg ggctggcgcg cggggagaat ccacgtgtcg
      121 tcgaggacga actggtgcgc gtgattccag cgcagttcat gaaccatgcg catcactggc
      181 tgatcctgca tgggcgctac gtctgcaagg ccagggtacc ggcctgtccg cagtgcgcga
      241 ttgctcgctg gtgcgaatac cagccgaaaa cctcgctctg aatgcggcga ggccatcgcg
      301 agccgtgtgg atcgcgatgg cctcgtggct tgatagccgc tgacgggatc ttagaagcgg
      361 aaacggaaac cgatggtcgg gtccgcgttg gtggtcttga tgtagccatt ggccagacca
      421 tccgacacgc tgctgtacat gacgcccgcg taggtatcaa aacgtttcgt ccagcggtag
      481 tccgcatcca ccgacaacac cgtttcagta ccgctgcagg tgccggccac cgttgaggag
      541 catcccgcga tgcccttgcc gacgccatag ctgttctgat cgtagccgta ccaggcagcg
      601 gtcagatcca gcttcttgcc ccacgtgtac ttcacgcccg cccacgccac ctgcagcacc
      661 ttttcggtcg ggaaggcggt gttgttgacg gcaccgagct tgtagccacc gatgtcatcg
      721 aaaccgatcg ccagagggtt cttcgggttg ccgaactgaa tgtgctcgta acccgcgtag
      781 accttcaccg ggatgaaccc gaggttgtac agccccagga tggtgaagct ctggttgtcg
      841 gaaatcgtgc cggaaacact gttgctggtg gacaggtagt tcggtgcagc caccgcgatg
      901 gcttcctgcg cagcgctcag cgcgctggcg gcaaccgcgt cgcgaacgtg cgcatacagc
      961 gcatcgaccg acaggcccgc gtagtcgaag ccgacatcga actgctctgc ggtgttcgca
     1021 gcgccaaatg actggctgaa tttgtacagg atgccgatgt gcaccgggcc gatggcttcg
     1081 ctgtacttga ccgagtcatc cagacgacgg tcctgcgtgt caccaccacc cgcggtggta
     1141 cctgacaggc cgatgataga gaacgcctgc gaggcggcat tcggatcgta cttggcgata
     1201 ccatccgcca gcagcgtgtt ctgacggccg aaagttaccg caccgaaggt cgccgaactc
     1261 agaccgaggt acgcctgctc gaacagctca ccggcgatac tggagtccag gttcgtcgtc
     1321 tgcgccgtca cggcgcgacc gttgttctgc gcgagtgact tcaacgcatc actgatgtcg
     1381 cccgaggacg ggttgaaata ggtttcgact cggccgatgc cgtaccagtc gcccacgccc
     1441 agcggctcca acacagccag gccaatgcgc gactggctca ggttgttgga ggtgaggccc
     1501 gtggccgagt ggttactgtc cttctgcacg atgtcagcgc tgctggccat gaagtagtcg
     1561 ctgaacggag ccccatgggt ctcgtactga aaaccgacgt cgatgatgcc gtagagcgtg
     1621 atgcccttgt acgtcaggct gtcgtccgac ggcgccatga ccgggccgac cgtgctttga
     1681 gcgctcgcaa cgccgctcac cgccgacagg gcacccaaca gagcaactcc gacggccaga
     1741 ttcacagcgc gacgaccgca ggctcccgat gcccccagcg cggagggcgc gtttgcatta
     1801 ttcacaaatt tactcccgtg gaccgattgt tggatttaat acacacattg ttacatatca
     1861 attaagaagc agcaaaactt agcataaatt ttcaatcaca gctcattctg tcggtgtctg
     1921 tttgtagcaa caccgggagc ttatgggttg attggacgtc tactcgatgc ggctttcccg
     1981 cgagccaaac ctgctggaaa tgagtcgcgc cagaacgctc agcagcagca cgaacagcgt
     2041 gatcagcagc gcccccgccc aggccagcga ctgccagttc ggatacggac tggtcgcgaa
     2101 ctgaaagatc acgaccggca gattcgctat cggctgattg aggttggcat tccagaactg
     2161 gttgttgagc gcggtaaaca gcagcggcgc ggtttcaccg ctcacgcgcg ccagcgctag
     2221 cagcactccg gtcagcagac cgctgcgggc ggccggatag atgatgctgg tgatggtccg
     2281 ccagggctgc gcgccgaggg cggccgacgc gtgccgcatg ttttgcggga tcagacgcag
     2341 catgtcctcg gccgtacgca ccgtcaccgg aatcacgatg attcccagtg ccacgacgcc
     2401 ggcaatcgcg gaaaaatgac ccatccgaac cacaacgagc gtatagacga acaggccgat
     2461 gacgatcgac ggcgcgctca gcagcacgtc gttgatgaat cggatcaccg tggacagccg
     2521 tgctgtgcgc ccgtattcgg acagataggt ggcggccagg attccgatcg gtccgccgat
     2581 cagcacgccc agcagtgtca tcatcagact gccgtagatc gcattgagca ggccgccgct
     2641 gctgccgggc gctggcgtgg tctgcgtaaa gaccgtccac gacagcccgc cgaaaccacg
     2701 cgcgaccaga gtccacagga tcgccgcgag gaacgccagc ccgatcgcgg tggcaacccc
     2761 ggaagcgccc agcgcaatcg acgctttgat gcgtcgtgtg cggatgctca tatgcgcgca
     2821 ccccgtcgct cgatctgcag cagcaacagt cgcgccgcgg cgatgatcgt gaaagtgatg
     2881 atgaacagca acaggccgag cgcgatcagc gacgaggtat agagagtgcc gactgcctcg
     2941 gtgaattcgt tggcgatact ggcggaaatg gtcgtacccg gggccaacag cgaggtcgag
     3001 atgcggtgcg cgttgccgac cacgaaggtc accgccatcg tctcacccag tgcgcggccg
     3061 aggccgagca tgatgccgcc gatgacgccg gcacgtgaat tgggcagcac cacatcccag
     3121 atgacttccc actgcgtggc gcccagcccg taagcggctt ccttcagcga tcgcggcgtc
     3181 gccagaaaga cttcgcgcat cactgaggta atgaagggca acaccatcac ggcgagcacg
     3241 atcccagcgg tgaacaatcc gatgccgaat ggcggaccgc gaaacagttt gccgagcaat
     3301 ggcagatcgc ccagcgtgtc gatcagaaac ggctgcaccg tgtgttgcag gatcggcgcc
     3361 agcacgaaca gaccccagat gccataaaca atgctcggca ccgccgccag caactcgacg
     3421 caagtggcaa tcggcccgcg cagccacagc ggacaggtct ctgtgagaaa caccgcgatg
     3481 ccgaacgaca acggcaccgc gatcagcatc gccaccagcg atgtgatcag ggtgccgtag
     3541 agcgccgcca gtgcaccgaa ctgctcagtg accggattcc acacttcccg ggtcagaaac
     3601 gcgaactgaa aggttttgat ggccggccag ccgccgtcgc acagcgccac catcacgccc
     3661 gcgagcaaaa tcagcaccag cgccgctgaa ccgatcgtca gccagcggaa tacgacctcc
     3721 gcgccagcct cgcgtttgag ctgcgcgtga aatggcagag tcgtcgccga cataacggga
     3781 tcgaaccctt agcgccgtcg tggctgcggt gcctggggct acttcgccgg ccaggcagcc
     3841 ttaccgccga ccttcaacga ggcagcccag gtcttctcga ccagcttgct gactgactcc
     3901 gggatcggca catagtccag atcctcagcc atcttggtgc cgctcttgta cgcccagtca
     3961 aagaatttca gggcagcgct tgtgttggcg tctttgctgt ccgcatgcat caggatgaag
     4021 ctcgcgccgg tgatcggcca actcgcagct cctgcctgat cggtgagaat caggtagtac
     4081 ccttccgcct tcgcccagtc ggcgctgctg gcggcggcct ggaacgcttt ggcttccggt
     4141 gcgacgtact tgccgtcgtg attcgccatc cgcacatagg tcatatgcgt ctgcttcaca
     4201 tacgcatact cgacgtagcc gatggcgcca ctggtttgtg tggtctggtt ggcgacaccc
     4261 tcgttgccct tggcaccaat gcccgacggc cactggactg aggtgttggc gccgaccttc
     4321 tgcttgaagc tcgggctgac cttggagagg tagtccgtga acaggaagtt cgttcccgag
     4381 ccgtccgatc gcgtgaccgg agcgatgtcg agcttcggca gattcaggga cggattgagc
     4441 ttcttgatgg ccggatcgct ccagcgcgtg atctcaccca gaaagatcgc tgccagggtt
     4501 gccccgtcca gttgcacatc gccgggcttg atcccaggaa tgttcacgac cggtacgacg
     4561 ccaccgatca ccatcggcca ttggacgagt ccgttctgct tcaggtcagc cggttccagg
     4621 ggcttgtcgg aagcaccgaa ggtcaccgtg cccgcttcga tctgcttgat gccgccgccc
     4681 gaaccgatcg actgataatt cagccctact ccggtctgcg ccttataggc ttcggcccac
     4741 ttggcataga tcggatacgg aaaggtcgct ccggcgcctg aaatatcatc cgcctgtgcg
     4801 tgaccgacga acgcacccga tagtgcgacc gcagcgatac cggcgcacat caatatgcga
     4861 ccagaccata tgcgaccaga ccatgaccca ggagtcgaag gtacgaagaa gttcatcagt
     4921 gcttcctctc tcaaagatat ctgataggta agaaaagaac gtgcgcgggg ttcagggccg
     4981 gtgcaaacgt tggccacccc cttacctgtc tctcggtccc ccgggccgca gcagtctgtg
     5041 tactttctgt gacagcgcgg cgacattggt gtgtcagttg tatgacaaca ttgcggtgaa
     5101 gggaaaaccc tttgtatttg ataacttacg ggttcggcgc aacactactg accgcatgaa
     5161 ccggcacgga atgtccgaat gcgcctgctg atcgttgaag atgacccgaa gctggcggac
     5221 ttcctggcgc ggggtctgcg ggccgagcgc ttcgcagtgg atgtcgcgcc cgacgggcag
     5281 caggggcaaa gctttctaga gtcttacgct tacgatttgc tgattctcga tctgatgctg
     5341 ccacggctca gcggcaccgc attgcttcag tggctgcgcc aacgccgcgc cgccctgccc
     5401 gtgctggtac tgactgcgcg ggatgccacg gaggataagg tcaagcactt tgaagccggc
     5461 gccgatgact atctgaccaa gccgttcgac ttcgccgagc tggtagtccg ggtgcgcgcg
     5521 ctcctgcggc gcagtccggt cgaacgtgcc gatgtgcttc ggattgcgga cctggagttg
     5581 aaccgcctga cacaacaggt gcgacgcggc gggcag
//
LOCUS       DATMRM010000199         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2594803, whole genome shotgun sequence.
ACCESSION   DATMRM010000199 DATMRM010000000
VERSION     DATMRM010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2594803"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VNZ06_05815"
     CDS             complement(<1..517)
                     /locus_tag="VNZ06_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20300.1"
                     /translation="MTTSETAPYAQLAEHAYQIRRNALRMGEVQGQGYIGQALDIADV
                     LAVAYFRAMRYRADDPEWEGRDRFLLSNGHYAIALYAALIEARIIADEELETYGSDDS
                     RLPMSGMASYTPGMEMSGGSLGQGLTIAVGRCLGLKRKGSDARVYTLFSDGELDEGAI
                     WEGLMSAAHWKL"
     gene            656..1546
                     /gene="gcvA"
                     /locus_tag="VNZ06_05820"
     CDS             656..1546
                     /gene="gcvA"
                     /locus_tag="VNZ06_05820"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HWW20301.1"
                     /translation="MHNRVTFKSIQAFEAAARLSSFALAADELFVTPSAISHQIKLLE
                     EQLSIRLFHRLHRTVILTDYGRQYAEEITAAFARIDSATREIGRAAKSDILTIHCTPS
                     FATQWLMPRIARFSAAHPDIDVRLNASSEAVDLISEAVDIDIRYGPRKLQPAGTMVLE
                     LPPETIVPLCSPALMAGEHPLRSVADLPHHALIHSEGCLVGWRDWMRLYRKTRLDIGR
                     GPRFDRSFMAISAAVDGLGVCLESLLLAQRELETGRLVAPFGLEGLPVHGYTLNLLKS
                     RAELPKLHSFQDWLFAELEK"
     gene            complement(1612..1923)
                     /locus_tag="VNZ06_05825"
     CDS             complement(1612..1923)
                     /locus_tag="VNZ06_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20302.1"
                     /translation="MDVSTETLVELLREVEADDPIDYADLPFGEQELRRLVMSSLVER
                     HHQVEAGNMSISDIHALYLLSTAKLVLENTVLHARLLLLQGQQVDVRSLLAPFTLKGK
                     P"
     gene            2058..2663
                     /locus_tag="VNZ06_05830"
     CDS             2058..2663
                     /locus_tag="VNZ06_05830"
                     /inference="COORDINATES: protein motif:HMM:NF020432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HWW20303.1"
                     /translation="MARITSVGIEQLRPLQATVGMLEVDAKRKRLLAMDAKSLKDFLK
                     SAPIPTVIGKHDRHYVIDHHHLARALWDGGIAKAFAEVVADLSRLPDAKFWDTVIERR
                     WVHPYDERGILRAISSIPDEVSALTDDPYRSLAAFVRDAGGYLKTPEPFAEFQWADFY
                     RTRMPVWTSESQFKAAVEQAVHLAWSPDARTLPGFKSRRAK"
BASE COUNT          565 a          853 c          874 g          589 t
ORIGIN      
        1 cgagtttcca gtgagcggcc gacatcagcc cttcccagat cgcgccttca tcgagttcgc
       61 cgtcggagaa cagcgtgtag acgcgggcat ccgagccttt gcgcttcagg cccaggcagc
      121 ggcccactgc gatcgtcaat ccctgcccga gcgagccgcc ggacatctcc atcccgggcg
      181 tatagctcgc cataccggac atcggcagac ggctgtcgtc actgccatag gtttccagtt
      241 cttcgtcggc gatgatgcgg gcctcgatca gcgcggcgta cagcgcgatg gcgtagtgac
      301 cgttcgatag cagaaagcgg tcgcggcctt cccattcggg gtcgtcggcg cgatagcgca
      361 tggcgcggaa ataggcgacg gcgaggacat cggcgatatc gagcgcctgg ccgatataac
      421 cctgcccctg cacttcgccc atgcgcagcg cgttgcggcg aatctgatag gcgtgctccg
      481 cgagttgggc gtacggcgcg gtttcgctag tggtcactgt ttgtctcctt atggatcgct
      541 ttgcaggggc cgctgtgatg ccgatcccag tgactcaagc gtaatctcgc gctcacacgc
      601 gctcaaacga attaagatgg gccaatggat gaattcaatt caccttgatg ctgccatgca
      661 caatcgcgtc accttcaagt cgatacaggc attcgaggcc gccgcgcggc tctcgtcgtt
      721 tgcgctcgca gcagacgaac tgtttgtcac gccctcggcc atcagccatc aaatcaagct
      781 gttggaagag caattgagca ttcgtctgtt ccatcggctg caccgtacgg tgatcctgac
      841 ggactatggg cggcaatacg ctgaggaaat caccgcggcg ttcgcgcgga tcgactcagc
      901 cacccgcgag atcgggcgcg ccgccaagag cgacatcctc acgatccatt gcacgcccag
      961 ttttgcgact caatggctga tgccgcgcat cgcgcgtttc agtgcggcgc atccggatat
     1021 cgacgtgcgc ttgaatgcct cttcagaggc ggtggatctg atctcggaag cggtggatat
     1081 cgacatccgc tacggtccga gaaagttgca gccggccggc acaatggtgc tcgaattgcc
     1141 acccgagacg atcgtgccgt tgtgctcgcc cgcgctgatg gctggagagc atccgctgcg
     1201 cagcgtcgcg gatttaccgc accatgcgct gattcatagc gaggggtgtc tggtcggatg
     1261 gcgagactgg atgcgtcttt atcgcaagac gcggctcgat attggacgcg gtccgcgttt
     1321 cgatcgatcg ttcatggcga tcagcgcggc agtggacggc ttgggcgtct gcctcgaaag
     1381 cctgcttctg gcgcagcgtg aactggaaac ggggcgactg gtggcgccgt tcggcctgga
     1441 aggacttcca gtacatggct atacgctgaa cctgctgaag tctcgcgccg aattgccgaa
     1501 gctgcatagc ttccaggatt ggctgtttgc ggaacttgag aagtgatgcg cgtgcggcac
     1561 tgatccggtg atgcgcaatg cgctgccggt gacagtgccg actaccggcc tttaaggttt
     1621 gcctttgagg gtgaacggcg caagcagtga ccgcacgtcg acctgctgcc cctgaagcag
     1681 aagcagcctc gcgtgaagca ccgtgttctc aagaacgagc ttagcggtgc tcagcagata
     1741 cagcgcgtgg atatcggaga tcgacatgtt gcctgcttcg acctggtggt gacgctcgac
     1801 aagtgaactc atgacgagcc gcctgagttc ctgttctcca aatggaaggt ccgcgtagtc
     1861 gatcgggtcg tcggcttcta cttcccgcag cagttccacc agcgtttcag tgctcacgtc
     1921 catgtgcagg ctccacgttg tcgttcaaga ccagcatagc caaacagagt caccagcact
     1981 gacagcctac cgtttctatt taagggagtc cagcttgagc agagcagtag aatgctccgg
     2041 atacggaggg gcacgccatg gcaagaataa cgagtgtcgg cattgaacaa ctgcggccgc
     2101 tgcaggcaac ggtcggcatg ctggaagtcg atgcaaaacg aaagcgcctg ctggccatgg
     2161 atgcaaagtc tttgaaagac tttctgaaaa gcgcaccgat tcccaccgtc atcggcaagc
     2221 atgacaggca ctatgtcatc gaccaccacc atctcgcgcg tgcgctgtgg gatggcggta
     2281 tcgcgaaagc gttcgccgag gtcgtagcgg acctgtcgcg gctgccggac gcgaagttct
     2341 gggacaccgt gattgaaagg cgctgggtgc atccctacga tgaacgcggc atcctgcgag
     2401 ccatttcctc gatccccgat gaggtttccg ccctcacgga cgatccgtac cgcagcctcg
     2461 ccgcattcgt gcgcgacgcg ggcggctatc tgaagacacc ggaacccttc gccgaatttc
     2521 agtgggcgga cttttatcgc acacgtatgc cggtctggac gagcgagtcg cagttcaagg
     2581 cagcagtgga gcaggcagtc caccttgcgt ggagccccga cgcccggaca ctgccgggtt
     2641 tcaagtcccg ccgcgcgaaa tagcgtcgag atagtcgccg gcaaagaaac cgttcgacgc
     2701 cccgaaaagt aagtcgctgg agcgtcgaac cgcagcgaag agcaacttat ccgtgtcgct
     2761 tcttcttcaa ctcgatcgtt tcgaataact catagttctg cgtcggtgtt tggtccgcac
     2821 ccggcgcgtg atagtagttc atcgttatcg tggtctcgcc acccggatgg cccggatcat
     2881 g
//
LOCUS       DATMRM010000200         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_103089, whole genome shotgun sequence.
ACCESSION   DATMRM010000200 DATMRM010000000
VERSION     DATMRM010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103089"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VNZ06_05835"
     CDS             complement(<1..786)
                     /locus_tag="VNZ06_05835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWW20304.1"
                     /translation="MSTALDSDYVDSYYAATAPRSAPFAPLSAGASTDVCVVGGGIAG
                     CSAALALAERGYQVVLLEAQRIGWGASGRSGGQVIFGLSADQADLEQWVGFEDARRIW
                     DISLAGIALLRQRIERHRIDCEWIDGEMFAAIKPRQWRALQTWQHELAHKYDYSGTRL
                     IEHAELCDLLATSRYRGALYDPLCGHLHPLRYTLGLARAAAQAGVRIHEGSRVLKFER
                     SAGRVQVHTENGSVSCGQLLLAGNAWLGGLVPLMQRKLMSIASY"
     gene            979..1725
                     /locus_tag="VNZ06_05840"
     CDS             979..1725
                     /locus_tag="VNZ06_05840"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HWW20305.1"
                     /translation="MSSKRPVIGVPADRRMIGAHPFHAVGEKYLTAILEAADAVPLII
                     PALAAQIRAEELLERLDGLLLTGSPSNVEPHRYGGEPSAPGTRHDPHRDDATLPLIPR
                     VIEAGVPLLGICRGFQEMNVALGGSLWQKLQEVPGNLDHRDDPKLELEQQYAPAHDVL
                     LEPGGVLRRLAGTDRIRVNSLHTQGVRELAKGLAVEARAPDGVIEAFRVRDARSFALA
                     VQWHPEWRASGNPFSRALFAEFGAACRGRT"
     gene            1789..>2905
                     /locus_tag="VNZ06_05845"
     CDS             1789..>2905
                     /locus_tag="VNZ06_05845"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWW20306.1"
                     /translation="MATEIQQFFRDHGIAEVEAIIPDMAGIARGKIMPAEKFAEDEGM
                     RLPESIFLQTVTGDYPPDTTEAMSQAEIDIVLKADPKTVRVVPWAAEPTAQVIHDCFY
                     SDGRRVMMAPRHLLRHVLELYAQRGWEAVVAPELEFYLIEPNTDADYQLKPPVGRSGR
                     SESGRQSYSIAAVNEFDPLFDDIYAFCEAQDIEIDTLIHEDGPAQMEINLLHGEPMDL
                     ADQAFLFKRTAREAALRHKMYATFMAKPHAKEPGSAMHIHQSILDKKTRKNIFSGEDG
                     TPSALFFSHIAGLQRYIPAAMSLFCSNVNSYRRMTRYLSAPINVQWGYDNRTAGLRVP
                     MSPPEARRIENRVGGADANPYIAIAASLACGYLGMVEG"
BASE COUNT          549 a          915 c          918 g          523 t
ORIGIN      
        1 gtagctggcg atgctcatca gcttgcgctg catgagcggt acgagacccc cgagccaggc
       61 attgccggcc agcaatagct gcccgcagga aaccgagccg ttttcggtgt gcacctgaac
      121 cctgccggcc gaacgctcga acttcagtac acgactgccc tcatgaatcc tcacgcccgc
      181 ctgcgcggcc gctcgcgcga ggccgagggt atagcgcaat ggatgcagat gaccgcacag
      241 cggatcgtac agcgcacccc gatagcgcga cgtcgcgagc agatcgcaca gctctgcatg
      301 ctcgatcagc cgcgtgccgc tgtagtcgta tttgtgtgcc agctcgtgct gccaggtctg
      361 cagtgctcgc cactggcgcg gtttgatcgc ggcgaacatc tctccgtcaa tccactcgca
      421 gtcgatccga tggcgctcga tgcgctgtcg cagcagagcg atgccggcga gggagatatc
      481 ccagatcctg cgcgcgtcct cgaagcccac ccactgctcg agatcggcct gatcggcgga
      541 tagaccgaag atgacctgtc cgccgctgcg cccggaggcg ccccagccga tgcgttgtgc
      601 ttccagcagc accacctgat agccacgctc ggccagagcc agtgccgccg agcatccggc
      661 gatgccaccg ccgaccacac agacatcggt gctggcgcct gcggaaagcg gcgcgaaggg
      721 cgcgctccgc ggcgccgttg ccgcgtagta gctatcaaca taatctgaat cgagcgccgt
      781 gctcatcggt gactattgcc agaaataatt ataaaaatca acagtttgac attcaaacgg
      841 agccgcggga gtccgtccaa aacaagccct gctcgacggc actcacccga gagctatggt
      901 ctaatgctta ccacacaaag cctgatggat cggcgcaatc tgtatatccg tacagctctt
      961 gtcccgggcc acgtcatcat gagttcgaaa cgaccagtca ttggtgtccc ggctgaccgg
     1021 cgaatgatcg gtgcgcatcc gtttcatgcg gtcggtgaga aatacctgac cgcgattctg
     1081 gaggccgccg atgcggtgcc gctgatcatt ccggcgctgg ccgcgcagat cagggccgag
     1141 gagctgctcg agcggctcga tggtttgctg ctcaccggca gcccgtcgaa cgtcgagccg
     1201 catcgctacg gcggcgaacc cagtgctccc ggcacccggc atgacccgca tcgtgacgat
     1261 gccacgctgc cgctgatacc gcgggtgatc gaggcgggcg tgccgctgct cggtatctgc
     1321 cgcggatttc aggagatgaa tgtcgcgctc ggcggctcac tgtggcagaa gctgcaggag
     1381 gtgcctggca acctggatca tcgcgacgat cccaagctcg aactcgaaca gcagtacgcg
     1441 ccggcgcacg acgtgctgct cgaacccggt ggcgtattgc ggcggctggc tggcaccgat
     1501 cgcatcagag tcaattcgtt gcacacccag ggcgtgcgtg agctggccaa ggggctggcg
     1561 gtcgaggcgc gcgcaccgga cggcgtgatc gaggcatttc gcgtgcggga tgcccgcagt
     1621 tttgcgctcg cggtgcagtg gcaccctgag tggcgcgcga gcggcaatcc gttttcgcgc
     1681 gcactgtttg cggagttcgg cgcagcctgt cgcgggcgca cctgacatcg tcaagccgat
     1741 cagggcgcgc ctttaggatg gcgcgcgacg aaccaataaa ggtgatctat ggcaaccgag
     1801 attcaacagt tctttcgcga tcacggcatc gcggaagttg aagcgatcat tcccgacatg
     1861 gcgggcatcg cgcgcggcaa gatcatgccg gcggagaagt ttgcggaaga cgaaggcatg
     1921 cgtctgcccg agagcatctt tctgcagacc gtgactggtg actatccgcc ggataccaca
     1981 gaggcgatga gccaggccga gatcgacatc gttctgaagg ccgatccgaa gaccgtgcgc
     2041 gtcgtgccct gggcggccga gcctaccgcg caagtgattc atgactgttt ctattccgac
     2101 gggcggcggg tgatgatggc gccgcggcat ctgctgcggc atgtgctcga gctgtacgcg
     2161 cagcgtggct gggaggcggt ggtcgcacct gagctcgagt tctatctgat cgaaccgaac
     2221 accgatgccg actatcagct caagcccccg gtgggccgat ccggtcgctc cgagtcgggc
     2281 cggcagtcgt acagcattgc cgcggtcaac gaattcgatc cgctgttcga cgacatctat
     2341 gccttctgcg aagcgcagga catcgagatc gatacgctga ttcacgagga cggcccggcg
     2401 cagatggaaa tcaacctgct gcacggcgag ccgatggatc tggccgatca ggcgtttctg
     2461 ttcaagcgca cggcgcgcga ggcggcgctg cgtcacaaga tgtacgccac cttcatggcg
     2521 aagccgcatg cgaaagagcc cggcagcgcc atgcacatcc accagagcat tctcgacaag
     2581 aagacacgca agaacatctt cagcggcgaa gatggcacgc cgtcggcgct gttcttctcg
     2641 cacattgccg gattgcagcg ctacatcccg gcggcgatgt cgctgttctg ctccaacgtc
     2701 aattcctatc gacgcatgac gcgctatctg tctgcgccaa tcaatgtgca gtggggctat
     2761 gacaaccgca cggccgggtt gcgcgtgccg atgtcgccgc cggaggcccg ccgcatcgag
     2821 aatcgcgtcg gtggcgctga cgcgaatccc tacattgcga tcgccgcttc gctggcgtgc
     2881 ggttatctcg gtatggtgga gggcc
//
LOCUS       DATMRM010000201         3721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2285286, whole genome shotgun sequence.
ACCESSION   DATMRM010000201 DATMRM010000000
VERSION     DATMRM010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2285286"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VNZ06_05850"
     CDS             complement(<1..237)
                     /locus_tag="VNZ06_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW20307.1"
                     /translation="MSDRPSLRVLLVEDSSLLAGRLAELIRRLPDVDLIDTVQTETDA
                     LTQINAAVPDVLILDLHLRSGSGFGVLRSLARDKA"
     gene            complement(400..1032)
                     /locus_tag="VNZ06_05855"
     CDS             complement(400..1032)
                     /locus_tag="VNZ06_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWW20308.1"
                     /translation="MPSILIADDHAMIRTGLRHYLEQDRTIEKIGETGTGAETLQQLR
                     EGNWDLVILDINMPDRSGIDILRHIRSGHPDTRVLVMSGFAEKQYAINVLRAGASGYL
                     AKDQAPEEFMRAVHTVLAGRRFVSATLSEMLVTALDEPTDQPLHASLSQREFQILCKL
                     AVGRSVSEIAQELFISVKTVSTYRARVLEKMNLDTNADLTTYALRNGLVQ"
     gene            1616..1948
                     /locus_tag="VNZ06_05860"
     CDS             1616..1948
                     /locus_tag="VNZ06_05860"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWW20309.1"
                     /translation="MKNDQILACVLTASLVTPVWSVAVEPDLDRATAVQFVEDSAITA
                     AIKTRLAEGQWSSLTQLDVGTDRAGTVWLSGTAKTQEAAERAVEMARDTDGVVQVRSH
                     IVVNRDSK"
     gene            2076..3311
                     /locus_tag="VNZ06_05865"
     CDS             2076..3311
                     /locus_tag="VNZ06_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWW20310.1"
                     /translation="MQARPEDPVESMRIPRGNGEAWLHSDTEDPPRARELISARALAR
                     PLWILASCALLVALRQTREVLIPLMLAILFTLILSGIVEALRRRHVPRGVSALILLLA
                     CGFALGGAVNALSAPAQEWMQNAPRVLRVIQHRVRPARALVSRLSDLAARTSALADGG
                     AEARAAPANAASGGVTAIDVLAETGSVLAGVVAVTALTLLLLSAGPGTLARMTAALGE
                     DLHAVQVLHTIDAIRVALGRYYGTLALINLSLGAATSVAMWLLHMPNPMLWGALAAVL
                     NFIPYLGSAVTLLVVSVVALVSFDSIPHVLLVSGTYLALATIEGQIVEPIFFGHRLDL
                     NPIVVFVALWLGGWLWGIAGVLFALPVLLAMKVAASQNGEHGVLVRFLGPARAAWLDG
                     KNATRAEIPSVLDARRRAR"
     gene            complement(3326..>3721)
                     /locus_tag="VNZ06_05870"
     CDS             complement(3326..>3721)
                     /locus_tag="VNZ06_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20311.1"
                     /translation="NALLRQRLRYSGLIIPWCTFCIPEIAHVGLQIWEAREKSVPVKT
                     YTMMMQDIDRAITDRQDTGFVKLHVVEGHDRIIGATIVASRASEMINEVCVAMHSGIG
                     LRRLGRVMHTYPSQASAICLAAMSMDEPC"
BASE COUNT          663 a         1155 c         1179 g          724 t
ORIGIN      
        1 cgccttgtcg cgcgccaacg agcgcaggac tccgaacccc gacccgctgc gcagatgcag
       61 gtccaggatc agcacgtccg gcaccgcagc gttgatctgc gtcagcgcgt cggtctcggt
      121 ctggacggta tcgatgagat cgacatccgg cagccggcgg atgagttcgg cgagacggcc
      181 cgccagcagc gacgaatctt cgacgagcaa cacgcgcagg gacggcctat ccgacatcgc
      241 tgatcagccc atcctgcatt ggggagcaca tcctattgtg gtgcctgcgc caatcgccca
      301 cagccaccct gaagcacaga caataatgcc gcagtgcgac aacgaatgca ggattgcgcc
      361 ctcgcggact gtcagcgctt cccgacggcc gcacgggatt tactgcacca gtccgttacg
      421 caatgcgtag gtcgtaagat ctgcgttcgt atcgagattc atcttctcaa ggacccgcgc
      481 ccggtacgtg ctaacggtct tcacgctgat aaacagctcc tgggcaatct ccgatacgga
      541 gcgtcccacc gctagcttgc acaggatctg aaactcgcgt tgcgacagcg aggcgtgcag
      601 tggctggtcg gtaggctcat ccagagctgt gaccaacatc tcactcaacg tagcgctgac
      661 gaagcgacgc cccgccagta ccgtgtgcac cgctcgcatg aattcttccg gcgcctgatc
      721 tttcgcaaga tagcccgaag caccggcgcg cagcacattt atggcgtact gcttctctgc
      781 gaacccactc atcacgagaa cgcgcgtatc aggatgcccg gagcggatgt ggcgcagaat
      841 atcgatgcca ctgcgatcgg gcatatttat gtcaagaatc accaggtccc agttgccctc
      901 gcgcagctgc tgcagcgttt cagctcccgt tcccgtctcg ccgatcttct cgatggtacg
      961 gtcctgctcc aggtagtgcc ttagtccggt cctgatcatc gcgtgatcat cggcaattaa
     1021 tatgcttggc attgaccctc ccgatcgcgc tagtaggacg tggataaccc gtacggtagg
     1081 cgtgacagta cctgctgcgc cgattttcaa gcgtacgcct tccgcctgtc cgagtgtagg
     1141 cctgctccta caatgtccta cgcgacggaa aatcggcgcg ccggcgctcc gggcacttat
     1201 ggcgcccgcc gatggccggg gcgaagcgtc gggagtcacc gacggttgtt tcgtcctgat
     1261 gagatgtggg gcgcgcgaca ccgcaccggc caggccgcaa gaagcaccgt tcatgcaccg
     1321 ttaatgtaac tcttcgctac ttctcgcggt cgcgttcatc gtgcgtatgc aagcagcgac
     1381 agcggacgcg tgttgtctgc gtgcagcgaa tacatgttgg tggtgcgaca cctgtactcg
     1441 tacgtaccgg cacactcggg cgtggtgtca tttacttaat gagaagaacg cgaacgctgc
     1501 gacggcgcac ccgtaggtcg cagcctacga ttgatgatgg tgagcgggga cggctaagcc
     1561 gacgcgcggc gggcaacatc ctacgcattc cgaacgcgca tgcaggaccg aagagatgaa
     1621 aaacgatcag atcctcgctt gtgtactgac agcttctctt gtcactcccg tgtggagcgt
     1681 cgcggtggag ccagacctgg accgcgccac ggccgtgcag ttcgtcgagg attccgccat
     1741 cacggcggct atcaagacca gattggccga aggacagtgg agcagcctca cgcagctgga
     1801 cgtcggcacc gaccgcgcgg gcaccgtgtg gctgagtggc actgcgaaga ctcaggaagc
     1861 ggccgagcga gcggtggaga tggcccgcga cactgatggg gtcgtccagg taagaagtca
     1921 catcgtggtc aatcgagact caaagtagcg acgccacgta cggcccctcg tgcggtgcac
     1981 ggacgcacca ggctttgcca gcgagggcca ggaacggggc ggcgattttc gccgccccgt
     2041 tcatgaggga aggcggcacg agcagggact taataatgca ggcacgcccg gaagaccctg
     2101 tcgagagcat gcgcattccg cgcggcaacg gggaggcatg gctgcattcg gacacagagg
     2161 atccgccgcg cgcgcgtgag ctcataagcg cccgcgcact ggcgcgtccc ctgtggattc
     2221 ttgccagctg tgcgttgctg gtggctttac gccagacgcg cgaggttctc atcccgctca
     2281 tgctggccat tcttttcacc ctcatcctgt ccggcatcgt cgaggccttg cgccgtcgtc
     2341 acgtgcccag gggtgtgagc gcgctcatcc tgttgctcgc ttgcggattc gctctggggg
     2401 gcgctgtcaa tgcgctctcg gcaccggcgc aggaatggat gcagaacgca cctcgtgtgc
     2461 tgcgcgtgat tcagcatcgg gttcggcccg cacgcgcgct cgtctcccgg ctcagcgacc
     2521 tggccgcgcg tacgtcggca ttagcagacg gcggcgctga ggcacgcgcc gcgccggcga
     2581 acgccgccag cggcggggtg acggcgatcg atgttctggc ggagaccggc tcggtcctcg
     2641 caggggttgt cgcggtgacg gcgcttactt tgttgctgct gtccgccggc ccgggtacgt
     2701 tggcgcggat gacggcggct ctgggggaag atctccacgc ggtgcaggta ctgcacacta
     2761 tcgacgcgat ccgcgtggcc ctaggccgct actacggcac gctggcactt atcaacctga
     2821 gcctaggtgc cgctacctca gtggcaatgt ggctcctgca catgcctaac ccgatgttat
     2881 ggggggcgct ggcggcggtg ctgaacttca tcccttatct gggttccgcg gtgacgctcc
     2941 tcgtcgtgag cgtggtcgcg ttggtcagct ttgactcgat cccccatgtg cttctggtga
     3001 gtggcactta tctggccctt gcgaccatcg aaggccagat cgttgaaccg attttcttcg
     3061 gtcatcgtct ggacctcaat cccatcgtcg tgtttgtggc cctgtggctg ggcggctggc
     3121 tgtggggaat cgccggggtg ctgttcgccc tgccggtgtt gctggcgatg aaggtagccg
     3181 catcccagaa cggcgaacac ggggttctgg tgcgcttcct cggaccggcc cgtgctgcgt
     3241 ggctcgacgg gaagaacgcg acccgcgcgg aaattcccag tgtgcttgac gcgcgccggc
     3301 gcgcgcgcta aggacccgcg ctcgcctaac aaggctcatc catactcatg gcggccagac
     3361 agatagcgga ggcttgcgag gggtaggtgt gcatcacgcg acccaggcgg cgcagtccga
     3421 tgccgctgtg catggcgacg cagacctcgt tgatcatttc actggcgcgc gaagccacga
     3481 tagtggcgcc gatgatgcgg tcgtggccct cgacgacgtg taacttcaca aaaccggtgt
     3541 cctgccggtc agtgatcgcg cgatctatgt cctgcatcat catggtgtag gtcttgacgg
     3601 ggacggactt ttcccgtgcc tcccagatct gcaggccgac gtgggctatc tcgggaatgc
     3661 aaaacgtaca ccacggaatg atgagtccgc tgtagcgcag acgctggcgc agcagcgcat
     3721 t
//
LOCUS       DATMRM010000202         3135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2595654, whole genome shotgun sequence.
ACCESSION   DATMRM010000202 DATMRM010000000
VERSION     DATMRM010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2595654"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /gene="nthA"
                     /locus_tag="VNZ06_05875"
     CDS             <1..610
                     /gene="nthA"
                     /locus_tag="VNZ06_05875"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR01323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HWW20312.1"
                     /translation="HPDHERSEVAEMDLRVRALRTVLAQKGYIDPAALDVLIDAYETK
                     IGPRNGARVVARAWVDPVFRKWLLEDATAAIASLGYAGRQGEYIVALENTESRHNMVV
                     CTLCSCYPWPVLGLPPTWYKSAPYRARAVRDPRGVLAEFGVTVPESTEIRVWDSTAEV
                     RYLVIPRRPPGTENMSEDQLADLVTRDSMIGTGLPQSTDTRS"
     gene            607..1269
                     /gene="nthB"
                     /locus_tag="VNZ06_05880"
     CDS             607..1269
                     /gene="nthB"
                     /locus_tag="VNZ06_05880"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR03888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HWW20313.1"
                     /translation="MNYRSYADLGGEEGHGRIVSEPEGELFHAPWERQALALTLAMGA
                     TGAWNNDMSRSARETLPGYRSLSYYEIWISGLERLLIAHSLVGTDEIAAGATLHPARS
                     GLRVLQAGAVPATLARGAPTQRPATRPARFAVGEQVRTRAAAVGHHTRLPSYARGKVG
                     RIDQVRGVHVFADTNAQGLGEQPQWLYTVVFNACELWGSDDARGQSVSIDAWEPYLEP
                     LS"
     gene            1266..1631
                     /locus_tag="VNZ06_05885"
     CDS             1266..1631
                     /locus_tag="VNZ06_05885"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HWW20314.1"
                     /translation="MNDAPAVLPAQRDRSGPVFREPWEAQAFAITLALHQRGLFSWSE
                     WAQALAAQIRAAQAAGDADLGGTYYRHWLTALEMLVAAKGASSIEELSRYQRAWSRAA
                     DRIPHGWPIDLEPEDLSNS"
     gene            complement(1897..2289)
                     /locus_tag="VNZ06_05890"
     CDS             complement(1897..2289)
                     /locus_tag="VNZ06_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWW20315.1"
                     /translation="MLLTTQAAVKHLGEGSSIINIGSVVTRITPPGSAVYTGTKGAVD
                     AITGVLSRELGARKIRVNALNPGMVETEGTHAAGFIGSDFEAGAVANTPLGRIGQPDD
                     IASIAVFLASDDSHWLTGEQLLAGGGVR"
BASE COUNT          587 a          939 c         1030 g          579 t
ORIGIN      
        1 gcatcccgat cacgagcgca gcgaggtcgc cgagatggac ctgcgtgtcc gggcgctgcg
       61 aacggtgctt gcccagaaag ggtatatcga tcctgcggct ctggatgtcc tgatcgacgc
      121 ctatgaaacg aaaattggcc cgcgcaacgg cgcgcgcgtc gtcgcacggg cgtgggtcga
      181 tccggtgttt cgcaaatggc ttctggagga tgcgaccgct gccattgctt cgctggggta
      241 cgcaggccgg cagggcgaat acatcgttgc gcttgagaac acggagtcgc gccacaacat
      301 ggtcgtctgc accctctgca gctgttatcc atggcccgtt cttgggctgc ctccgacttg
      361 gtacaagagc gcgccatacc gcgcgcgtgc ggtaagggat ccacgcggcg tattggccga
      421 attcggtgtc actgtgcctg aatcgactga aattcgtgtc tgggactcga cggccgaggt
      481 gcgctacctg gtgataccgc gacgcccccc agggacggaa aacatgtccg aggaccagct
      541 cgcggacttg gtcacgcggg actcgatgat cggcacggga ctgccgcagt ctacagacac
      601 tcgctcatga actatcgatc ctacgcagac ttaggtggcg aggagggaca cggccgaatt
      661 gtttctgagc ccgagggtga gttgttccat gccccctggg agcgacaggc cttggcgctg
      721 actctcgcga tgggggccac cggtgcttgg aacaacgata tgagccgcag tgcccgggag
      781 accctgcctg gctaccgctc gctgagctac tacgaaatct ggatcagcgg tctcgagaga
      841 ctgttgatcg cacatagcct ggttggcacg gacgaaatcg ccgcaggcgc gacgctgcat
      901 ccggcacgat cgggactgcg ggttttgcaa gcgggtgccg tgccagcgac gctggcgagg
      961 ggagcaccca cgcaacggcc ggctacgcga ccggcacgtt ttgccgtagg cgaacaggtg
     1021 cgcacgcgcg ccgccgcggt cggccatcat acgcggctcc cgagttacgc tcgaggcaag
     1081 gtcggtcgca ttgatcaggt gcgcggtgtg cacgtattcg ctgacacaaa tgcgcaggga
     1141 ctcggtgaac agccgcagtg gctgtacacg gtggttttca acgcgtgtga gttgtggggt
     1201 agtgacgacg ctcggggcca aagcgtgtcg atcgatgcat gggagccgta tctggagcct
     1261 ctgtcgtgaa cgacgcaccc gctgttcttc ctgcccagcg agaccgcagt ggtcccgtgt
     1321 ttcgcgagcc atgggaagcg caagcgtttg caataacgct cgcgctgcat cagcggggtc
     1381 tcttcagctg gtcggaatgg gcccaggcac tggctgcgca gatcagggcg gcacaagccg
     1441 cgggcgatgc tgacctcggc ggtacctatt accgtcactg gctcacggcc ctcgagatgc
     1501 tggtcgcagc caaaggcgcg agctcgatcg aagagctgag ccggtaccag cgggcgtgga
     1561 gccgcgcggc cgatcgcata ccgcacgggt ggccgatcga cctggaaccc gaggatttgt
     1621 ccaattcctg attccaggaa tcatttctcg cagagcatcg atctggatct tggcctgagt
     1681 ggggtcgagg actgacgaag ggccaacaga agtgctgtca ggcgacccga ccgccaacgg
     1741 gttgacgatc ttgagcctgc gatcgttcgg ccggcgttca gatcctcgct tgtgtggctt
     1801 gcatcttctg aacaacaagt caaccgcgta ctgggcaaat gcctggatga attcgaggcg
     1861 caccccgcgt gcggaatgcg ccccctaaag tgcccctcag cgcacgccgc caccggcaag
     1921 caactgctct ccggtcaacc agtgtgagtc atccgaggcc aggaacaccg caatcgacgc
     1981 gatgtcgtcc ggctgaccga tgcgacccag cggggtgttt gcgacggcgc cggcttcaaa
     2041 atcggagccg atgaagccgg ccgcgtgagt gccttcggtt tccaccatgc ccgggttcag
     2101 cgcgttaacg cgaatcttgc gcgcgccaag ttcacgcgac agcaccccgg tgatcgcatc
     2161 caccgctccc ttggtgccgg tgtataccgc gcttccggga ggcgtgatgc gagtaacgac
     2221 ggacccgatg ttgatgatgc tggagccttc gccaaggtgc ttgaccgccg cctgggtcgt
     2281 cagcagcatg ccgagcacat gccttgtcgg atcctgtgcg cgtcgcaatc tatgcggagc
     2341 tggcggcttc ggcaggcgcc acgatctgct cgaactttca ccaggtcagc gaccgtagca
     2401 tcctcaattc cacgctttca caacatttca gggcgctcag ggaagcggga ttggtccgca
     2461 gcgaacggca cggggtggag atgcgcaacg tcagccgctg caaggagatt gagcggcgct
     2521 tccccggatt aatcgccgca attctgaacg cgcatagcgt ccaggcgaca ggccgagctg
     2581 gtgatgtgag gcggaagccg gccggccgga gggcgaaagc tgtttagtga atggtgggcg
     2641 gtacttaacg tcacgatcga actgtcccag aaatgtccca gacacgcgat ccggacgggc
     2701 tacctgtggc ccgaaccgga agtcgggact gtccgcaggc gcagcctact tgccggtgcc
     2761 ggtcgcaggg ccgggcgtta tcgctctgtg gctttgcttt caagtacgcg atcacgtcga
     2821 cgcgatcgtg tgcctgcatg agccgaaagt atatcttgct accagcagcg gatgcgcccc
     2881 gaggccacga ttcgtggcct cggggcgcat cttttcctgg agtaccgtca ggtccgatct
     2941 atcgatcatc agcatcgcga ccagtggaag cgggcaccgc aggggcgacc gcgacgcgga
     3001 agcctgttag gacccaggct cgctgactcg gtgcgaagcg cggtgcgagc aatccgtcgt
     3061 tgatctcacg cgagacagca aagcggttaa atgcggttgc tgggagaaac agccgcgtat
     3121 tgtccggaaa gccgt
//
LOCUS       DATMRM010000203         5606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3206171, whole genome shotgun sequence.
ACCESSION   DATMRM010000203 DATMRM010000000
VERSION     DATMRM010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3206171"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VNZ06_05895"
     CDS             complement(<1..376)
                     /locus_tag="VNZ06_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWW20316.1"
                     /translation="MRALVTGATGFVGAAVVRALLNDGWEVRGLARPHSDRTNLRDLT
                     LEVSIGDLADTSSLDRALAGCKALFHVAADYRLGVRDPEQLYRTNVEGTRAMLVAAGR
                     AGVERVVYTSSVATLGIPKDGSP"
     gene            complement(373..1083)
                     /locus_tag="VNZ06_05900"
     CDS             complement(373..1083)
                     /locus_tag="VNZ06_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20317.1"
                     /translation="MSRVGIVAALATEARALTPIIPHRGCPFGLEDGTLLNVSGIGNT
                     AAKDAALALINEGADALVSFGLAGGLDPDVKAGTIFLPAEVLAAGSERFPTSDAWRNR
                     VAIALVAFRPVAAGKMLTSLREVSDIAAKQAMFRESGAMAVDMESAAVARVATAHGLP
                     FLIARVIVDTATDVLPAAVMAAGGSGELRVWRLIAALTRAPAEIVDVIRLARRFRVAK
                     SALRAVGQSRSLREASVS"
     gene            complement(1080..3074)
                     /gene="shc"
                     /locus_tag="VNZ06_05905"
     CDS             complement(1080..3074)
                     /gene="shc"
                     /locus_tag="VNZ06_05905"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HWW20318.1"
                     /translation="MTPNVPQRALVPGTTPSTRPDAGAPHLRVLDDAFPLDNTIAAAR
                     DALVAQQSAEGYWLFELEADCTIPAEYILMMHFLGEIDDTLQSRIASYLRAHQGEHDG
                     WPLYHNGDFDMSCSVKAYMALKLAGDSPDAPHMARARAAILERGGAARSNVFTRITLA
                     LFAQVPWRAVPYIPVEIMLFPRWFPFHLSKVSYWSRAVMVPLFILCTRKPVARNPRKV
                     GIRELFTTPPDKERNYLNGAGHQGSFLARVFRVLDRVGRAADPVIPKTLRARAMQRAE
                     SWMLERLNGEDGLGAIFPAMVNALEAMVVLGYPTDDPRRVTAKHALEKLVVSNVTNAY
                     CQPCVSPVWDSALACLAMQATGGATAHTAWGRGLDWLRTKQLLDKPGDWRERRPHLRG
                     GGWAFQFGNGHYPDLDDTGAVVWALHQADDPARYGFTITRALDWLVGMQSSNGGFAAY
                     DVDNTYYYLNEIPFADHGALLDPPTSDVTARVVAAMARADRAQDKSALERAIRYLRRE
                     QEDDGSWFGRWGTNYIYGTWSVLCALALAGVSGHDPAVRRAVSWLAGQQNSDGGWGES
                     NDGYRLEGDRRQPASTPHQTAWALLALIASGETGSVHVRRGIDYLMRTQQADGLWSDP
                     HFTAPGFPRVFYLRYHGYCAYFPLWALAAYRNATRPGTTH"
     gene            complement(3071..4285)
                     /locus_tag="VNZ06_05910"
     CDS             complement(3071..4285)
                     /locus_tag="VNZ06_05910"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HWW20319.1"
                     /translation="MGLQATIGPEREGDISQPVRSDSPDSDECYQDRILPEVSRTFAL
                     TIPQLPPALRGPVTSAYLLCRIADTIEDEPALSPAETLAYLQRFKAVVSASADPSALA
                     NDLSRRLSNQTLDSERELVRNMERVVRVTQRLEPAGRAAIGRCVRLMCDGMPQFQLSA
                     SLQGLGTASDLDDYCYYVAGVVGEMLVELFCSYSPRIAQQREALYELATSFAQGLQMT
                     NILKDQWEDRRRGACWLPQEVFARHGIDVAQLSPQQHDRRFAAGLRELIGIAHAHLRN
                     ALTFTLLIPRDETGIRRFCLWAIGLAVLTLRKISANPAFVCGTQVKVTRTTVTVTRVL
                     TSVAVGNNWMLKRLFARAARGLPLAPSCDVRLTGAARMRAAMEDEGAPVQSLVSIRAG
                     AMRPDLKGINAS"
     gene            complement(4362..>5606)
                     /locus_tag="VNZ06_05915"
     CDS             complement(4362..>5606)
                     /locus_tag="VNZ06_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HWW20320.1"
                     /translation="AAEQNTAPPSTVAITPPAPEPKPSVFSPSYWFNPAIAPFLPVPE
                     VATDPDSGTTVGLLAVKLNTDDNGDIRRIVAPDFLYNPYFGYGGHARIYDYPSSDVQW
                     SAIAGAKERVERGVDLEYQSGILRDRTWSFTYSLVYDRDGTPRFFGVGNSSPAIAETD
                     YTEQQEYAEAQIGYNLTHSWQLVYTALARSVDVLPGTLAGIDSLTTRFGRILGVGTNN
                     EILNRFSIVYDTRDSVTIPSVGMKWVIYTGLASRRGVFNDSLYSEAGVDGRVYVPITA
                     RTVLAMHMDLRYLPDTHRLPFWALSALGGSESDIGQSQPLRGFGPGRFYDRDAFSASA
                     EIRQQVASISAISTHVELELAPFVDLGRVFARPSIIPLSKLHTVGGLGIRGLARPFVV
                     GYIDVGYGDEGVAVFTGIYYPF"
BASE COUNT         1133 a         1772 c         1679 g         1022 t
ORIGIN      
        1 ccggtgagcc atctttgggg atgccgagcg tcgccacgct gctggtgtat acgacgcgtt
       61 cgacacccgc cctacccgca gccaccaaca tcgcgcgcgt gccttcgaca ttggtccgat
      121 agagttgctc cggatcacgc acacccaagc gatagtctgc cgcaacgtga aacagcgcct
      181 tgcaacccgc gagcgcccgg tcgagtgagc tggtatccgc caggtcgccg atcgacacct
      241 ccagcgtcag atcgcgcaga ttggttcgat cggagtgagg ccgggccagc cctcggactt
      301 cccacccatc attcagcagc gccctgacta ccgctgcgcc gacgaaaccc gttgctccgg
      361 tcaccagcgc tctcatgaca ccgaggcttc gcgcaacgaa cggctctgac cgaccgcgcg
      421 caacgcggac ttcgcgactc ggaaacgtcg agccaaccgg atgacgtcaa cgatctcagc
      481 aggagctcgc gtcaaggctg cgatcaaacg ccagactcgc agttcgcccg agccgcctgc
      541 ggccatgacc gcagctggca agacatcggt cgctgtgtcg acgatcaccc gcgcgatcag
      601 aaatggcagt ccatgcgccg tcgcgacccg ggcgaccgcg gcgctctcca tatcgacggc
      661 catcgcacct gattcgcgaa acatcgcctg cttcgcagcg atgtcactga cctctcggag
      721 gctggtcagc attttgcctg cggcgactgg ccggaaggcc accagtgcga tcgcgacccg
      781 attgcgccat gcatcggagg ttgggaatcg ctcgctgccc gccgccaaga cttcggcggg
      841 cagaaaaatc gtgcccgctt tcacatccgg atcgagtcct ccagccagcc caaagctaac
      901 caaggcgtcg gctccctcgt tgatgagtgc aagcgccgca tccttggcag cggtgttgcc
      961 gataccgctg acgttcagca gagtgccgtc ctcgagtccg aaaggacacc cgcggtgcgg
     1021 gatgatcggt gtcagcgcgc gagcctcagt ggcgagcgcc gcgacgatac cgactcgact
     1081 caatgcgtcg ttccgggacg cgtggcattg cggtatgcag cgagcgccca cagcggaaaa
     1141 tacgcgcaat agccgtggta acgcagatag aacacgcgtg ggaatccggg cgctgtgaag
     1201 tgcgggtcac tccatagacc atcggcttgc tgcgttcgca tcaggtagtc gatgccgcgc
     1261 cgcacgtgta ccgacccggt ctcccctgaa gcaatcagag ccagcaacgc ccaggctgtt
     1321 tggtggggtg tgctggcggg ctgccgacgg tcgccttcca ggcgatagcc atcgttgctc
     1381 tcaccccagc cgccgtccga gttctgttga ccggcaagcc agcttaccgc acgccggacc
     1441 gcagggtcgt ggccgctcac acctgccaac gcgagcgcac acaggacgga ccaggtccca
     1501 tagatatagt tggtacccca ccggccgaac caggatccgt cgtcctcttg ttcgcgacgc
     1561 agatacctga tcgctcgctc cagcgcggac ttgtcctgcg ctctgtcggc acgcgccatg
     1621 gcagcaacga cccgagccgt cacatcgctg gtgggtggat cgagcaaggc accgtggtcg
     1681 gcgaatggaa tctcattcaa gtagtaatag gtgttatcga catcgtaagc ggcgaagccg
     1741 ccgttgctgc tttgcatgcc aaccagccag tcgagtgccc gggttatcgt gaaaccatag
     1801 cgcgccggat cgtcagcctg atgcaatgcc caaacgacag ccccggtgtc gtcgagatcc
     1861 ggatagtggc cattaccaaa ttggaacgcc cagccgccgc ccctcaagtg tggacgccgc
     1921 tcgcgccagt cccctggctt atccagcaac tgcttggttc gcagccaatc cagtcccctg
     1981 ccccaggcgg tatgggctgt cgcgccgccg gtggcttgca tcgccaggca ggccaatgcg
     2041 ctatcccaga ccggcgaaac acacggttgg caatacgcgt tcgtgacgtt gctgacgacg
     2101 agtttctcga gcgcatgttt cgcggtcaca cggcgtggat catcagtggg atagcccagg
     2161 acgaccatgg cttccagcgc gttcaccatc gcgggaaata tggctcccaa accatcctct
     2221 ccattcagtc gctcgagcat ccagctctcg gcgcgctgca tcgcacgtgc tcgtagcgtt
     2281 ttagggatca cagggtctgc ggcacgaccc actcgatcca ggactctgaa gacgcgtgcc
     2341 agaaagctgc cctgatggcc cgcgccgtta agataattgc gctccttgtc cggcggcgtc
     2401 gtgaaaagtt cgcgaatacc caccttgcgc ggatttctcg ccaccggctt gcgagtacac
     2461 agaatgaaca gcggcaccat cacggcccgc gaccagtacg acaccttgct gaggtgaaac
     2521 gggaaccagc gcggaaacaa catgatttcc actggaatat aggggaccgc ccgccacggc
     2581 acctgcgcaa acaaggcgag agtgatgcgc gtaaagacat tggaccgtgc cgcgccgcca
     2641 cgctcgagga tggcagccct ggctcgcgcc atatgcggtg catccggact atcgcccgcc
     2701 agcttcaaag ccatgtaggc cttgaccgag caactcatgt cgaaatcgcc gttgtgatag
     2761 agaggccatc cgtcgtgttc gccctgatga gctcgcagat agctggcaat gcgcgactgc
     2821 aacgtgtcat ctatttcgcc gaggaaatgc atcatcagga tgtattccgc gggaatcgtg
     2881 caatcggctt ccaactcgaa tagccaatat ccttccgcgc tctgctgcgc gactaaggcg
     2941 tcgcgcgcgg ctgcgatcgt attgtcgagc gggaaggcat cgtcgagtac tcgcaggtgc
     3001 ggtgcaccgg cgtcggggcg tgttgacggt gttgtgcctg gtaccaaggc acgctgtgga
     3061 acgttcggag tcacgatgcg ttaattccct tcaggtcggg gcgcatcgcg cccgcacgta
     3121 tcgacaccag actttggacc ggcgcgcctt catcttccat ggctgcgcgc atgcgcgccg
     3181 cgccggtgag gcgaacgtcg cacgagggtg ccagaggcag cccacgggca gcacgcgcga
     3241 acagtcgttt cagcatccaa ttattaccga ctgccacgct ggtcagcacg cgagttaccg
     3301 taaccgtcgt acgggtaacc ttcacctgcg tgccgcagac gaaggccgga ttggcgctga
     3361 tcttgcgcag cgtcagtaca gccaggccga tcgcccacag gcagaagcgg cgaatccccg
     3421 tctcatcgcg tgggatcagc aatgtaaacg tcaaagcgtt gcgcagatga gcgtgcgcga
     3481 ttccgatcag ctcgcgaaga ccggccgcga agcgcctatc gtgttgttgc ggggaaagct
     3541 gcgcaacgtc gatgccatga cgcgcgaaga cctcttgtgg cagccagcat gcaccacgcc
     3601 gccgatcctc ccattgatct ttcaggatat tcgtcatttg cagcccctgt gcaaatgagg
     3661 tggccaactc gtagagcgcc tctcgctgtt gcgcgatcct tggcgagtaa ctgcaaaaca
     3721 gttcgaccag catttcgccg acgactccgg caacgtagta gcaatagtcg tccagatcgc
     3781 ttgcagtgcc aagaccttgc aaactggcgg atagctggaa ttgaggcatg ccatcgcaca
     3841 tcagccgcac gcagcgcccg atggcggcgc ggcccgcagg ctccagtcgt tgggtcactc
     3901 gaacgacgcg ctccatgttg cgcaccaact cgcgctctga atccaaggtc tggttggaca
     3961 gccgacggga caggtcgttg gcgagcgccg aagggtccgc gctagccgac accacggcct
     4021 tgaatcgctg caaataggcc agtgtttcag cgggtgaaag tgcgggttca tcctcgattg
     4081 tgtcagcgat acgacataac agataggcgc tggtcacggg tccacgcaac gcagggggca
     4141 gttgtggaat ggtcagcgcg aacgtgcgcg agacctcggg caggatgcga tcctgataac
     4201 actcatcgga atcgggcgaa tccgagcgga ccggctgcga tatgtcgcct tcacgctcgg
     4261 ggccaatggt tgcttgtaac cccatgcttt acttatagcg ttcaccgcgc ggcaccgcaa
     4321 ccgtgaccct tgcgtagaca taccgcgagg cgccttcagg tctaaaacgg atagtagata
     4381 cccgtgaaga cggcgacacc ctcgtcgcca tagccgacat cgatgtagcc cacgacgaac
     4441 ggcctcgcca ggccacgaat gcccagcccg cctacggtat gcaatttcga tagcggaatg
     4501 atgctgggcc tggcgaacac cctaccgagg tccacgaacg gcgccaattc gagctcaacg
     4561 tgggtcgaaa tggcgctgat actggcaact tgctgacgta tctccgcgct cgcagaaaac
     4621 gcatcgcgat cgtagaagcg ccccgggccg aatccgcgca acggctggct ttggccgatg
     4681 tcgctctcgg agcctcccag cgcgctgagg gcccagaacg gcaggcggtg tgtatcgggg
     4741 aggtatcgca gatccatgtg catggccaga acggttctgg ccgtgatcgg aacatagacg
     4801 cggccgtcta cgcccgcctc gctatataga gaatcattga agacgccgcg gcgactggca
     4861 agcccggtgt agatcaccca cttcatgccc accgatggaa tggtgacact gtcgcgagtg
     4921 tcgtagacga tcgaaaatcg gttcaggatc tcgttattgg ttcccacgcc gagaatccgg
     4981 ccgaagcgcg tcgtcagcga atcaattcca gccaaagtcc ctggaagcac gtccactgaa
     5041 cgtgcgagcg ccgtatagac cagttgccac gaatgggtca ggttgtagcc gatctgcgcc
     5101 tcggcgtact cctgttgttc ggtgtagtca gtctctgcga tcgcgggcga gctgttaccg
     5161 acgccaaaga aacgcggcgt gccatcgcgg tcataaacca ggctgtaggt aaaagaccaa
     5221 gtgcggtcgc gcagaatacc gctctggtac tcgaggtcca caccgcgctc gacacgctcc
     5281 ttggcaccgg caatggctga ccattgcacg tcgctcgatg gatagtcgta gatacgcgcg
     5341 tgtccgccat atccaaagta cgggttgtag agaaaatccg gcgcaacgat gcggcgaatg
     5401 tctccgttgt catcggtatt cagtttaacc gccagcaacc ccaccgtcgt accactgtcc
     5461 ggatcggtgg ccacctcggg tactggcagg aacggcgcga tcgcaggatt gaaccaatag
     5521 gacggactga agacgctcgg ctttggctct ggggcaggcg gggtgatcgc gacagtggat
     5581 ggcggtgcgg tgttctgttc agcggc
//
LOCUS       DATMRM010000204         2055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2596772, whole genome shotgun sequence.
ACCESSION   DATMRM010000204 DATMRM010000000
VERSION     DATMRM010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2596772"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1728
                     /locus_tag="VNZ06_05920"
     CDS             <1..1728
                     /locus_tag="VNZ06_05920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWW20321.1"
                     /translation="CLVQYPQHAQLCVAIGNNPVAGGFIYAVGSFASGALVTHELGDL
                     DLEHAHRLAVDVALRGRSYRWIAPLESEGTRAHTRFSARLAGFDVDPDGQIAERPDHD
                     FRGWLWQDSRCLGETVPNESEGCLKRSFFSVRLPVGLFSEELRDNAIVWPPKDLAQIQ
                     VRIRALGPGDGPALFDSNQPGANAPFALSDLKAQLLAGESLRIRRLDTQVPSDVISLS
                     YEADAAVSGPHLLHRLVARLPVPTGDQPLSARQIITTPVGEFEVLLTGDPHSADSTLA
                     LLAGRLALFAAAILAAILLTWAGIEQRIIHRITLLTQRAASVRQSVDRSQGLHLDLQG
                     LRGSDELSLMAGVLSDLLQRVNDDAKREQLRIEQEKNMWHAVGHEIMAPLQSLTALHA
                     SPQDPSVRYVNRMRQAVRVLYGSASPSDAFQSASLSMQTLDVNAFLRTVANNAAHAGI
                     DQVRYDGQSGAVIVKADEYSLEDVVTHVLNNADRHRTRETPIRMTLRASPGTADVRIH
                     NRGAQIHPAMLGKIFEYGVSDADSGEAGSHRGQGLFVAKTYMAKMGGTIEAVNVEDGV
                     EFVLKLALA"
     gene            complement(1679..>2055)
                     /locus_tag="VNZ06_05925"
     CDS             complement(1679..>2055)
                     /locus_tag="VNZ06_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20322.1"
                     /translation="PRAQIEALFRSQAQAMEEQGCRGCALGNAAVEISDDDELLARVV
                     RDYKGEVRNRLRTMARELGALNPAALGDSLMLLMDGAYFTRLAFARGDGPAQAIISAA
                     NSLIDAATPKRASARTRRRPRR"
BASE COUNT          350 a          677 c          672 g          356 t
ORIGIN      
        1 tgcctggtgc agtatccgca gcatgcgcag ctgtgtgtcg cgatcggcaa caatccggtg
       61 gccggcggct tcatctatgc ggtcggctca ttcgccagcg gggcgctggt cacccacgag
      121 ctgggtgatc tggatcttga gcatgcccat cgtctggcgg tcgatgtcgc actgcgcggc
      181 cgcagctatc gctggatcgc cccgctcgag tcggaaggga cccgcgcgca cacgcgattc
      241 agcgcccggc tcgccggctt cgacgtcgat cccgacggtc agatcgccga gcggcccgac
      301 cacgattttc gcggctggct gtggcaggac agtcgttgcc tcggggagac ggtcccgaat
      361 gagtcggagg gctgcctcaa gcgttcgttc ttttcagtgc gactgccggt ggggctgttc
      421 agcgaagaac tgcgcgacaa cgcgatcgtc tggccgccaa aggacctggc gcagatacag
      481 gtgcgtatcc gggcgctggg tccgggcgat ggccccgccc tgttcgacag caatcagccc
      541 ggggcaaacg cgcccttcgc actgtcggat ctgaaggcgc agctgcttgc cggcgaatcg
      601 ctgcggatcc gccgtctcga cacccaggta cccagcgatg tgatctcgct cagctacgag
      661 gcggacgcag cggtgagcgg gccgcacctg ttgcacaggc tggtcgcccg cctgccggtg
      721 ccaaccggcg atcagccact gtcggcccga cagatcatca cgacaccggt gggcgagttc
      781 gaggtgctgc tgaccggtga tccgcacagt gccgacagta cgctggcgct gctcgccggg
      841 cggctggcat tgttcgccgc ggcgatcctg gccgcgatcc tgttgacctg ggcgggcatc
      901 gaacagcgca tcatccatcg cattacgctg cttacccagc gcgccgcttc ggtcaggcag
      961 tcggtagatc gctcccaagg cctgcatctg gacttgcagg ggctgcgcgg cagcgacgaa
     1021 ttgagtctga tggccggtgt cctgtccgat ctgctgcagc gggtcaacga tgatgccaag
     1081 cgcgagcaac tgcgcatcga acaggaaaag aacatgtggc acgccgtcgg ccacgagatc
     1141 atggcgccgc tgcaatcgct caccgcgctg cacgcctcgc cgcaggatcc gagcgtgcgc
     1201 tacgtcaatc gcatgcgtca ggcggtgcgg gttctgtacg gcagcgcctc tccgagcgat
     1261 gcgtttcagt cggcctcgct gtcgatgcag acgctggatg tcaatgcgtt cctgcgaacg
     1321 gtcgccaaca atgccgcgca tgccggcatc gatcaggtgc gctacgacgg gcagtcaggc
     1381 gcggtgatcg tgaaggccga tgaatattcg ctggaggacg tggtcaccca cgtgttgaac
     1441 aacgcggatc ggcatcgtac gcgcgagacg ccgatccgga tgacactgcg cgccagtccg
     1501 ggcacggccg atgtgcgcat ccacaatcgc ggcgctcaga ttcatccggc aatgctcggc
     1561 aagatcttcg agtatggcgt ctccgatgcc gattccggcg aggccggcag ccaccgcggc
     1621 cagggcctgt tcgtcgccaa aacctatatg gcaaagatgg gcggcacgat tgaggcggtc
     1681 aacgtcgagg acggcgtcga gttcgtgctg aagctcgctt tggcgtagcc gcgtcaatca
     1741 gcgaattggc ggcactgatg atggcctgcg ccggcccgtc gccgcgcgcg aacgcgaggc
     1801 gcgtgaaata ggcgccatcc atcagcagca tcagcgaatc acccagcgcc gccgggttca
     1861 acgcacccag ttcacgcgcc atggtccgca gacgattcct gacctcgccc ttgtagtcgc
     1921 gcaccacccg cgcaagcagc tcgtcgtcat cggaaatctc caccgcggca ttgccgagcg
     1981 cgcagccacg gcagccctgc tcctccatcg cctgcgcctg cgagcgaaac agtgcctcga
     2041 tctgtgcccg gggac
//
LOCUS       DATMRM010000205         2979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3206253, whole genome shotgun sequence.
ACCESSION   DATMRM010000205 DATMRM010000000
VERSION     DATMRM010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3206253"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1369
                     /locus_tag="VNZ06_05930"
     CDS             <1..1369
                     /locus_tag="VNZ06_05930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HWW20323.1"
                     /translation="PLNKGRVAEGTFLGEPRALAYLAFTEAWERFSYYGMTALMVLYL
                     TQSLLLPGHVEHIQGFRGFRAALESVYGPLNSEALASQIYGLYTGFVYFTPVLGGWIA
                     DRWTGPRNAVALGAILMSAGHIAMAFDASFLLALLLLVVGCGFLKGNISAQVGNLYRE
                     DDGAGRTRGFAIFSFGINVGAVAGPLLCGLLAQKYGWHVGFGLAGLLMLIGLFTYLAG
                     YSKYTVSIRAAPRPIQPTPLTRRQYKVIAALILVMALTVFQSIAYYQNSDMGMIWIDR
                     YVDLNAWGFHVPVAWFNSIDPFASMLAVPLLLAHWRSQAARGREPAEIGKIVIGAWMA
                     CVANLLLVAGCAFMHPVPAVVPVLYDTLLGVAFLYYWPTLLALVSREAPPRVRATLMG
                     TAFLSLALADVLVGQLGAFYGRMSPLQFWALEAGVAAVGAVLAMLLARPLARLFAANL
                     EQAAA"
     gene            complement(1389..2897)
                     /locus_tag="VNZ06_05935"
     CDS             complement(1389..2897)
                     /locus_tag="VNZ06_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWW20324.1"
                     /translation="MPSTPSVGSDGVEIGSPEVVIYATSKRGLSISMDLARLRASIDR
                     TWDTSILDQLKAYIRIPNKSPHFDVNWERSGHMEKAAQLMADWCRAQSVPGMRVEIRR
                     LPGRTPLLLVDVPGESPDCVLLYGHLDKQPEFTGWLPGLGPWEPVIRDGRLYGRGGAD
                     DGYAVFSSLTAIAALKQQKVPLARCVLLIEACEESGSTDLPAHLQALGTAIGDPSLVV
                     CLDAECSDYDRVWCTTSLRGNLTGQLRVRVLTEGVHSGMGTGIAPTPFRILQQLLARI
                     EDPNTGEILPEELRVQIPSDRRAQISQSAQTLRGAIAAKLPWAQSVRAVSEDPAELIA
                     NSTWRATLAVTGADGLPAVASAGNVLLPEIALKLSLRLPPTCDAERATRGVRSILEKD
                     PPYGAQVSFEDASGTDGWNAPRFAPWLEQSIMQASQAVYGRNAMHAGSGGTIPFMAML
                     GHQFPRTQFFITGVLGPHSNAHGPNEFLDIEYVKRLTACVSLVLADHARTRT"
BASE COUNT          484 a          929 c          944 g          622 t
ORIGIN      
        1 cccgctgaac aagggccgcg tcgctgaagg tacttttctc ggcgagccca gagcgctggc
       61 gtatctggcc ttcacggaag cctgggagcg cttttcctac tatggcatga cggcgctcat
      121 ggtgctgtac ctgactcagt cgctgctgct gccagggcat gtcgagcata tccagggctt
      181 caggggtttt cgcgccgcgc tcgaatcggt ctatggtccc ttgaattccg aggccctcgc
      241 ctcgcagatc tatggcctct ataccgggtt tgtctacttc acgccggtgc tgggcggttg
      301 gatcgcggac cgctggacgg gcccgcgcaa tgccgtggcg ttaggcgcga tcctgatgag
      361 tgcggggcat atcgcgatgg ctttcgacgc ctcctttctg ctggcactgc tgctgctcgt
      421 cgtgggctgt gggtttctca agggcaacat ctccgcccag gtcggcaatc tctatcgcga
      481 ggacgacggc gccggccgta cgcgcggctt cgccatcttt tcgttcggga tcaacgttgg
      541 ggcggttgcc ggtcccctgt tgtgtggcct gctcgctcag aagtacggct ggcacgtcgg
      601 cttcggactg gcagggttat tgatgctgat cggactgttc acctacctgg ccggctattc
      661 caaatacacc gtctcgatac gtgcagcgcc gagaccgata caacccacgc cgctgactcg
      721 acgacagtac aaagtgattg ccgcgctgat tctggtgatg gcgctgaccg tctttcagtc
      781 gatcgcgtac taccagaatt cagacatggg aatgatctgg atcgaccgct atgtcgatct
      841 gaacgcgtgg ggctttcacg ttccggtggc gtggttcaat tcgattgatc cgttcgcgag
      901 catgctggcg gttcccttgc tgttggcaca ttggcgctcg caggcggcgc gtggccgcga
      961 accggcggag atcggcaaga ttgtgatcgg tgcgtggatg gcgtgcgtcg ccaatctgct
     1021 gctggtggcg ggctgcgcct tcatgcaccc ggtaccggcc gttgtgccgg tgctctatga
     1081 cacgctgctc ggcgtggcgt ttctgtacta ctggccaacg ctgctggcac tggtatcgcg
     1141 cgaagcgccg ccgcgcgtgc gcgcgacgct gatgggaacc gccttcctgt ccctggcctt
     1201 ggcggatgtt ctcgtggggc agttgggcgc gttctacggg cgtatgagcc cgttgcagtt
     1261 ctgggcgctg gaggcagggg tggccgcagt cggtgcagtc ttggcgatgt tgctggccag
     1321 gccgctcgcg cgcctgtttg ccgccaacct cgagcaggcg gccgcctagc gccataccgc
     1381 aatcgcgatc aggttcgagt tcgggcgtga tcggccagta ccagcgacac acaggcggtc
     1441 agccttttca catactcgat atcgagaaac tcattcgggc catgggcatt cgaatgcgga
     1501 cccaggacgc cggtgatgaa gaactgtgtg cgcggaaact gatgtcccag catcgccatg
     1561 aaagggatcg tgccgccgct gccggcgtgc atcgcattcc tgccgtagac ggcctgggag
     1621 gcttgcatga tcgattgttc gagccacggc gcgaacctgg gcgcattcca cccatcggtg
     1681 cctgaagcgt cctcgaagct cacctgcgcg ccgtagggag gatctttctc cagaatgctg
     1741 cggacgcccc gagtggcacg ctcggcgtca caggtgggcg gcagtcgcaa cgacagcttg
     1801 agtgcaatct cgggcagcag cacattgccg gccgaggcga cggccggcag tccgtcagcc
     1861 ccggtgactg cgagggtcgc gcgccaggtg ctgttggcga tcagttcggc cggatcctcc
     1921 gacacggcgc gcacgctttg agcccacggt agtttggccg cgatcgcgcc gcgcagcgtc
     1981 tgcgcgctct ggctgatctg cgcccgtcga tccgacggaa tctgcacccg cagctcttcc
     2041 ggcaatatct cgccagtgtt cggatcttcg atcctggcca gcaactgctg caggattcga
     2101 aacggcgtcg gtgcgatgcc ggtacccatg cccgaatgca ccccttcggt gagcacccgc
     2161 accctcagct gtccggtcag gttgcctcga agcgatgtgg tacaccacac ccgatcatag
     2221 tcgctgcatt cggcgtccag acacacgacg agggatggat caccgatcgc tgtccccagg
     2281 gcctgcaggt gagcgggcag atcggtgcta ccgctctctt cgcaggcttc aatcagcagc
     2341 acacagcgcg ccagcggtac cttttgctgc ttcagggcgg caatggccgt cagtgagctg
     2401 aacacggcgt agccgtcgtc cgcaccaccg cgaccgtaaa gccggccgtc gcggatcacc
     2461 ggctcccatg gaccaagtcc cggcagccag cccgtgaatt ccggttgctt gtcgaggtgt
     2521 ccatagagca gtacgcagtc gggactttca cccggtacgt ccaccagcag cagcggcgtg
     2581 cggcccggca gacgcctgat ttcgacccgc atccccggga ccgactgtgc ccgacaccag
     2641 tcagccatca actgagccgc cttctccatg tggccgctgc gttcccagtt cacatcgaaa
     2701 tgtggtgact tgttcgggat ccgaatgtag gccttgagct gatccaggat cgaggtgtcc
     2761 caggtcctgt cgattgacgc gcgcagtctt gccagatcca tgctgatgga gagccctcgt
     2821 ttcgaggtcg cgtagattac gacttcaggg gagccgatct ctaccccatc tgagccaacc
     2881 gagggcgtcg acggcatgcc agtgagtgat cttccgataa actccccgaa atatgatcga
     2941 tcgtctgctg tctgtggccc cgatgatgga ttggaccga
//
LOCUS       DATMRM010000206         6463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2286183, whole genome shotgun sequence.
ACCESSION   DATMRM010000206 DATMRM010000000
VERSION     DATMRM010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2286183"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VNZ06_05940"
     CDS             complement(<1..368)
                     /locus_tag="VNZ06_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20325.1"
                     /translation="MSALAMPERHSPVVPQIQWHRVLRTVLRSRALDELEESRLVPAR
                     KVLYQFTARGHDVTQPMLAQLLTGFSDAASVYYRSRPLMLGLGLSLDDALASTMMRSG
                     GVSEGRDIGVVFNLPKHGEV"
     gene            481..951
                     /locus_tag="VNZ06_05945"
     CDS             481..951
                     /locus_tag="VNZ06_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWW20326.1"
                     /translation="MKLDRIDARLLTELQQDGRRSVVELSECIGLSGTPCARRIRQLE
                     HNGVIQGYTAVVDPVRLGLSVQAFVQVRLERQTDDNVAHFQHELQSIDAVVSCYAMTG
                     EYDFLLQVVTTDLDVLSNIVLKKLLSIRSVRDVHSSIVLQTIKRSMRLPLAHLV"
     gene            complement(911..2230)
                     /locus_tag="VNZ06_05950"
     CDS             complement(911..2230)
                     /locus_tag="VNZ06_05950"
                     /inference="COORDINATES: protein motif:HMM:NF002204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HWW20327.1"
                     /translation="MSSRTRALLISAPASGQGKTTVTAALARRARQRGWRVRVFKTGA
                     DFIDPMILERACGAAVYQLDLWMVGRAQCQRLLRQAASEADLILVEGVMGLFDGDPSS
                     ADLAQQLGLPVLAVIDASAMAQSFGALALGLQQYRPALTLQGVLANRVAGSAHAAMLA
                     QSLPPSLRWFGALPQDRGLALPERHLGLTQAAEVDDLDARLDRAAAALGECANLETVP
                     STQFPAAPEAPPPPRRLQGVRIAIARDVAFSFIYPANLDWLRAMGASLRFFSPLSDRA
                     LPPADALYLPGGYPELHAATLAANAPMLAALRAQVNAGTAVVAECGGMMLLLQSLTDV
                     DGREHAMAGVLPGGTCMQPSVQSLALQSVRFDAGELRGHSFHFSRLATPLIARQRGRT
                     QHGAEGEGVYRAGAVTASYIHFYWPSNPAAAASLFRPDAPVADASSA"
     gene            complement(2236..2802)
                     /locus_tag="VNZ06_05955"
     CDS             complement(2236..2802)
                     /locus_tag="VNZ06_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20328.1"
                     /translation="MNARVAILSVLSLWMLATRFGHFGSRWSLPDASWAIFFVGGFYL
                     SRCWRSAFAWLLGLAVGIDLSVIQFYGVSNYCLTPAYAFNLPAYAVLWFSGAWLQRHL
                     RGELADLLRLGASAFIALSVCFVISNGSFYWLGGRVRTVSLAGWTLNFADWYASYLGN
                     ALLYIAIACVVQVALTRRAGASRAWRPH"
     regulatory      complement(2930..3159)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3179..4318
                     /locus_tag="VNZ06_05960"
     CDS             3179..4318
                     /locus_tag="VNZ06_05960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF008398.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HWW20329.1"
                     /translation="MPVPNSIADLRALAERRIPRAIFDYADRGAFDELTIDRNRRDLQ
                     ALQFRQRVMRDTSKTSLATTLVGEPASIPLAIGPTGSCGLFYGDGEIHGCRAAQDFGI
                     PFCLSTMSICSIEDVHAAVGKPFWFQIYLMKDRGFNQELVERAKAAGCSALMLTVDLP
                     ILSLRRRDAKNGMSVPPRLTWQNALEIAVRPAWAARVLLGKRRTFGNLVSVARGKSAA
                     SLAQWTSAQFDPGITWKEVEWLRSLWPGKFIIKGILDSEDAQIAAGFDIDALVVSNHG
                     GRQLDGAPSTISVLPEIVQTVNGRCEVWFDGGVQSGQDILRALALGARGCLIGKSFLY
                     ALAALGGPGVTLALKIMRQELQVSMALTGCTDVQQVDARILRPRS"
     gene            4408..5115
                     /locus_tag="VNZ06_05965"
     CDS             4408..5115
                     /locus_tag="VNZ06_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWW20330.1"
                     /translation="MPEAAAPVDSLKILLIEDDPQTADFIVRGLEEASHSISVAHNGR
                     DGLARALAERWDLLVIDRMLPEIDGLQIVQSLRQSDSSAAVLLLTTLGGVDDRVQGLN
                     AGADDYLNKPFAFSELLARVTSLGRRTRRHTPETALRIANLEMNLLTRTVVRGAEHIE
                     LMPREFQLLEYLMRHADQTVTRSMLLENVWDVHFDPNTNVVETLVSRLRGKIDRGNAV
                     PLIHTIRGVGYSLRDPK"
     gene            5150..>6463
                     /locus_tag="VNZ06_05970"
     CDS             5150..>6463
                     /locus_tag="VNZ06_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWW20331.1"
                     /translation="MYAAITAISFVVLFGVIFWSTTRFMRHQIDDSVSSEIDEIRTDA
                     RGMSQPQTESLVRDLAKHSSGFSYLLQDRNGVARAGNLPALDPQLGIREWSVPAAQRS
                     SARASIRGQGVSLGEDYLFVGWSTHQLHEMEEMVIGTFVWGFAAAIALALAGGFIMSG
                     RLLRKIQSISDTSRNIVETDLSRRIPLSEARDEFDNLSTSVNSMLDRIQALMSDLSQV
                     TTDIAHDMRTPLTRLRQRLELAQRSEVDAASLRAALASTVREIDSILEIFAALLRIAQ
                     IESGARSGAYRQVDLNEVISTVAELYRPSAEDRQQTLSEQLSADVSVNGDRELLMQLF
                     ANLTQNAVLHCPAGSKIVLLTRQSERGPQVLVADNGSGIPAEMRDKVLQRFFRLEKSR
                     TTPGNGLGLSLAAAIVKLHDASMSFSDNGPGLRVTVSFAHPRPAPD"
BASE COUNT         1206 a         2134 c         2042 g         1081 t
ORIGIN      
        1 cacacttcgc catgcttcgg caggttgaac accacgccga tgtcgcgacc ttcgctgaca
       61 ccacccgagc gcatcatcgt cgaggccagc gcatcatcga gcgacaatcc aagccccagc
      121 atcagcggac gcgagcggta atacaccgat gcggcatcgc tgaatccggt cagcagctgc
      181 gccagcatcg gctgcgtcac gtcatggccg cgcgccgtga attggtagag cacctttctg
      241 gcgggcacca gccggctctc ctccagctca tccagcgcac gcgatcgcag cacggtacgc
      301 agtacccgat gccactgaat ctgcgggacg accggcgaat ggcgctcagg catcgctagg
      361 gctgacatgc cttgtaaggg tacgctcagt tgggagtaaa aatgactaat ttacgttgtc
      421 attcctcata tgggtagtga taaactccaa tttctcagca ttggatagtc actttcactc
      481 atgaagctcg accgaatcga tgcccgtcta ctcaccgagc tgcaacagga cggccggcgc
      541 agcgtggttg aactgtccga gtgcatcggc ctgtcgggca ctccctgcgc gcggcgcatc
      601 cgtcagctcg aacacaacgg cgtcatccag ggttacaccg ccgtggtcga tcccgtccgc
      661 ctcggactga gcgtgcaggc gttcgtgcag gtacggctcg aacgccagac cgacgacaac
      721 gtcgcgcact tccagcacga gctgcagtcg atcgatgcgg tggtgagctg ctacgccatg
      781 accggtgaat acgatttcct gctgcaggtc gtgaccaccg acctggacgt gctcagtaac
      841 atcgtgctga agaaactgct cagtatccgc agcgtacgcg atgtgcactc gagcatcgta
      901 ttgcagacga tcaagcgctc gatgcgtctg ccactggcgc atctggtctg aacagactgg
      961 ccgccgcggc cgggttggac ggccagtaga agtgaatata ggaagccgtg acggcaccgg
     1021 cgcgatacac accctcgccc tcggcgccat gctgcgtgcg gcctcgctgc cgcgcgatca
     1081 gcggcgtcgc gagtcgcgag aagtgaaagc tgtgtccgcg cagctccccc gcatcgaacc
     1141 gcaccgactg taacgccagc gactgcaccg aaggctgcat gcatgtgccg cccggcagca
     1201 cgcccgccat cgcgtgttca cgtccgtcca catcggtgag tgactgcagc agcagcatca
     1261 tgccgccgca ctcggccacc accgctgtgc cggcattcac ctgtgcgcgc agcgccgcca
     1321 gcatcggtgc gttggccgcc agcgtcgcgg catgcagctc cgggtaaccc cccggcagat
     1381 acaacgcatc cgccggcggc agtgcacggt ccgacagcgg cgagaaaaat ctcagcgacg
     1441 cgcccatcgc ccgcagccaa tccaggttcg ccgggtagat gaatgaaaac gccacatcgc
     1501 gcgcgattgc gatacgaacc ccttgcagcc gccgcggcgg cggcggcgcc tcgggcgcgg
     1561 ctggaaactg cgtggacggc accgtttcca gatttgcgca ctccccgagc gctgcggccg
     1621 cgcgatccag gcgcgcgtcc aggtcgtcca cttccgcggc ctgcgtcagt cccagatgcc
     1681 gctcgggcag ggcgaggcct cgatcctgcg gcagcgcgcc gaaccaccgc agcgagggcg
     1741 gcaggctctg cgccagcatc gcggcgtgcg cgctgccggc cacgcgattg gcgagcacgc
     1801 cctgcagcgt cagtgccggc cgatactgct gcagccccag tgccagcgcg ccaaagcttt
     1861 gcgccatggc gctggcatcg atgaccgcga ggaccggcag acccagctgc tgcgccaggt
     1921 ccgcactgga cggatcgcca tcgaagagtc ccatcacacc ttccaccaaa atcagatcgg
     1981 cctcagaggc ggcttgccgc agcagccgct ggcactgcgc gcggcccacc atccacagat
     2041 cgagctgata gaccgcagcc ccgcaagcac gctcgaggat catggggtcg atgaaatccg
     2101 cgccggtctt gaatacccgc acacgccagc cacgctgccg cgcgcggcgc gcgagcgctg
     2161 ctgtcacagt ggtctttccc tggccggagg caggcgcgga aatcagcagc gcacgtgtgc
     2221 gggaagacat gcgcttcagt ggggtcgcca ggcgcgcgag gctccggcac ggcgggtaag
     2281 cgccacctgc accacgcagg cgatcgcaat atagagcagc gcattgccca ggtagctggc
     2341 gtaccagtcg gcgaagttca gggtccagcc cgccaggctc actgtcctca cccgcccgcc
     2401 cagccagtag aaactgccgt tgctgatcac gaagcacacg ctcagcgcga tgaacgcact
     2461 ggcaccgagg cgcagcaggt cagcgagctc gccccgcagg tgtcgctgca gccaagcccc
     2521 gctgaaccac agtaccgcat aggccggaag attgaacgca taggctggtg tcagacaata
     2581 gttactgacg ccgtagaatt ggatgaccga gaggtcgatc cccaccgcca gcccgagcag
     2641 ccatgcgaac gcgctgcgcc agcagcgcga cagatagaaa ccgccgacga agaagatcgc
     2701 ccaggaagca tccggcaacg accagcggct gccgaaatgg ccgaagcggg tcgccagcat
     2761 ccacagactc aataccgaaa ggatcgccac tcgagcattc atgctgccat ctcccaaagt
     2821 ttcgcgctcc acgcgcgata aatgttgatg gatcgatgac gtgcgaagga agattgcaga
     2881 acaggtgccg gcaggacgca ggcacatggt ctggcaacgc ccagcgcgtg ccatccgtac
     2941 tgttccaggc cggtctccgg gcttgcgagt ggagtcgtga tcgcttccgg tgacggcacc
     3001 ttcccatgct catcgagcac agtggtctca acgccgtcgc tcgaactcgc ctaccgttgc
     3061 gtgggcagcg ccggcattgc catccgcatt gcgcgatgac tcaccggctt cccgaacacc
     3121 tgtaacgcgc ggcactgtac ctgccagata tcggcgcagc aagtacaatg cgccgcctat
     3181 gccagtcccc aacagcatcg cggatctgcg cgcattggcc gagcgccgca tcccgcgcgc
     3241 catcttcgac tacgcggatc gcggcgcctt tgatgaactg acgatcgatc gcaaccgccg
     3301 cgacctgcag gcgctgcagt ttcgccagcg cgtcatgcgc gataccagca agacctcgct
     3361 cgccacgacc ctggtgggcg agccggccag catcccgctg gcgatcggac cgaccggctc
     3421 gtgcggcctg ttctatggcg atggcgaaat ccacggctgc cgcgcggcgc aggacttcgg
     3481 tattcccttc tgtctgagca ccatgtcgat ctgttcgatc gaggacgtgc acgccgctgt
     3541 cggcaagccg ttctggtttc agatctatct gatgaaggac cgcggcttca atcaggagct
     3601 ggtcgaacgc gccaaggccg ccggctgctc ggcgctgatg ctgacggtgg atctgccgat
     3661 cttgagcctg cgccggcgcg atgcgaagaa tggcatgagt gtgccgccgc gactgacgtg
     3721 gcagaacgcg ctggaaatcg cggtgcggcc ggcgtgggcg gcgcgggtgc tgctcggcaa
     3781 gcgccgcacc ttcggcaatc tggtgtcggt cgcgcgcggc aagagcgccg cgtcgctggc
     3841 gcagtggacc tcggcgcaat ttgatcccgg tatcacctgg aaggaagtgg agtggctgcg
     3901 ctcgctgtgg cccggcaagt tcatcatcaa gggcatcctc gacagcgagg atgcgcagat
     3961 cgcggccggc ttcgatatcg acgcgctggt ggtctccaac cacggcgggc ggcagctgga
     4021 cggtgcgccg tcgacgatct cggtgctgcc ggagatcgtg cagaccgtga acggacgttg
     4081 cgaggtgtgg ttcgacggcg gcgtgcagtc cgggcaggac atcctgcgcg cgctggcgct
     4141 cggtgcgcgc ggctgcctga tcggcaagag ctttctgtac gcgctggccg cgctgggcgg
     4201 tccgggtgtc acgctcgcac tcaagatcat gcgacaggag ctgcaggtca gcatggcact
     4261 caccggttgc accgatgtcc agcaggtcga tgcccgtatc ctgcgaccgc ggagctgatg
     4321 gatcgtgacc ggcttcgtga cccatctgga gtgcgcccac tgcgccacgc actatgagcc
     4381 caacgcggtg cacgggttgt gcacccgatg ccagaagccg ctgcgccagt ggattcactg
     4441 aagattctgc tgatcgagga tgacccgcag acggccgatt tcatcgtccg cggcctcgaa
     4501 gaagcctcgc attcgatcag cgtcgcgcac aacggtcgcg atggcctggc ccgggcactg
     4561 gcagagcgct gggacctgct ggtgattgac cgcatgttgc cggagattga cggcctgcag
     4621 atcgtgcagt cgctgcgcca gagtgacagt agcgcggcgg tactgctgct gaccaccctg
     4681 ggcggagtgg acgatcgggt gcagggcctc aacgccggtg cggatgacta tctgaacaaa
     4741 ccgttcgcat tctccgaact gctggcacgg gtcacctcgc tcggccggcg cacgcggcgc
     4801 cataccccgg agaccgcgct gcgcatcgcc aacctggaga tgaacctgct cacccgcacc
     4861 gtggtgcgcg gcgcagaaca catcgagctg atgccgcgcg agtttcagct gttggagtac
     4921 ctgatgcggc atgccgatca gaccgtgacg cgcagcatgc tgcttgaaaa tgtctgggat
     4981 gtgcacttcg atcccaacac caatgtggtc gagacgctgg tcagtcggct gcgcggcaag
     5041 atcgatcgcg gcaacgcggt gccgttgatc cataccatac ggggcgtggg atactcgctg
     5101 cgtgacccga agtagggtac ttcgcacttc cagctttcgc ctcgcactca tgtacgcggc
     5161 gatcaccgca atcagcttcg tggtgctgtt cggcgtcatc ttctggtcca ccacgcgctt
     5221 catgcggcat cagatcgatg acagcgtatc gagcgagatc gacgagatcc gcaccgatgc
     5281 gcgcggcatg agccagccgc agaccgaatc cctggttcgc gacctcgcga aacactcctc
     5341 gggcttctcc tacctgctgc aggatcgcaa cggtgtggca cgcgcgggca atctgccagc
     5401 gcttgatccg cagctcggca tccgcgaatg gagtgtcccg gccgcgcagc gctcctcggc
     5461 gcgtgcctcg atccgtggcc agggcgtgtc actgggcgag gattatctgt tcgtcggctg
     5521 gagtacgcat cagctgcacg agatggaaga gatggtcatc ggcaccttcg tctgggggtt
     5581 tgccgccgct atcgcactgg cgcttgccgg cgggttcatc atgagcggcc gtctgctgcg
     5641 caagattcag tcgatcagcg ataccagtcg caacatcgtg gaaaccgatc tgagccggcg
     5701 catcccgttg tccgaggcgc gcgatgagtt cgacaacctg agtaccagcg tcaacagcat
     5761 gctcgaccgc attcaggcgc tgatgagcga cctgagccag gtgaccaccg acatcgcgca
     5821 cgacatgcgc acaccgctca cccgactgcg gcaacgactg gagctggcgc agcgatcgga
     5881 ggtggacgcg gcctcgcttc gcgccgcgct cgcatccacc gtcagggaaa tcgattccat
     5941 tctggaaatc tttgccgcgc tgctgcgcat cgcacagatc gagagtggcg ctcgctccgg
     6001 cgcctaccgc caggtggatc tgaacgaggt gatctccacg gtcgcggagt tgtaccgccc
     6061 atcggcggaa gacagacagc agaccctgag cgaacagctg tccgcggacg tgagcgtcaa
     6121 cggcgaccgt gagctgttga tgcaactgtt tgcgaatctg acgcagaacg cggtgctgca
     6181 ctgcccggcc ggatcgaaga tcgtgctgtt gacgcggcaa tcggagcggg gaccgcaggt
     6241 gctggtcgcg gacaatggca gcggcatacc ggctgagatg cgcgacaagg tcctgcaacg
     6301 cttctttcgg ctggagaaaa gccgcaccac accgggcaac ggcctgggac tgagtctcgc
     6361 cgccgcgatc gtcaaattgc acgatgccag catgagcttc agcgacaacg gaccgggtct
     6421 gcgggtcacc gtctcgtttg cccacccgcg gccagccccc gat
//
LOCUS       DATMRM010000207         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3835381, whole genome shotgun sequence.
ACCESSION   DATMRM010000207 DATMRM010000000
VERSION     DATMRM010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3835381"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="VNZ06_05975"
     CDS             <1..1047
                     /locus_tag="VNZ06_05975"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWW20332.1"
                     /translation="FNTRVLLGTVTGVNTAAQRVQIGEHDIPYDYLVLATGAAHSYFG
                     RDEWQPYAPGLKRIEDATAIRGRLLRAFERAEATEDDEERRALLTFLIVGAGPTGVEL
                     AGAIVELARFGMEKEFRRFDPASARVILVQSGPRILPAFHERLSAIARRSLERMGVEV
                     LLDSRVDHIDASGVSIGGKRVAARTVLWAAGVTASPAAKWLQAAADPAGRVKVQADLS
                     IPGHDNIFVIGDTALSVAWNKQPVPGLAPAAKQGGEYVAKLIRARVSGRRPPANFVYR
                     HAGSLATIGRQAAVADFGRIKLWGILAWWLWGVIHIGFLLGVRNRMATMINWFWSYLT
                     FAGGIRLITGEDET"
     gene            complement(1025..1726)
                     /locus_tag="VNZ06_05980"
     CDS             complement(1025..1726)
                     /locus_tag="VNZ06_05980"
                     /inference="COORDINATES: protein motif:HMM:NF023168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HWW20333.1"
                     /translation="MKDPKPTSNQHDRAAFSGRDVIYRDGAINALDVQRAADALLRQG
                     IKPSVAALREQLGGGSPNTLTPLLAKYWETLGQRLVTGPEALERVPESLARVTELLWR
                     RAIEEARERLKILQGSAQPTGGDSAALQEQILKLSVALAEAGAREGEQLTHLASLSKE
                     REGLRTERTSLLTVLKSTQVLLQHQNARVAALEQERARTLTAASRPKQARHRKRSKRS
                     SARKGKPRARSRPHP"
     gene            complement(1769..>2596)
                     /locus_tag="VNZ06_05985"
     CDS             complement(1769..>2596)
                     /locus_tag="VNZ06_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWW20334.1"
                     /translation="DIGTGMLNALGIVSALYHRTQSGEGQKIETSLFSTGVALQAQQM
                     IQIANLDAEQHRHEVETLKTAYAAGKKHTEVVDDFANSRLRADLPRTARPVEVPDCLH
                     RPTDRQMVPYYRVYQTRDGYISIAALHRGQRRKVCGALGLEDPDCDTNPVDLSDEQYY
                     QQKSLMRVIEGRLRTQPNAYWLQKFEAAGVPCAPVNYRADLYTDPQAAALGLIWDLNN
                     RDLGKYKASGHPIRFSKTPVLPGKGAPVVGEHSDPLLREFGYSDAQIAAFKASGVVK"
BASE COUNT          448 a          798 c          817 g          533 t
ORIGIN      
        1 ttcaataccc gtgtactgct cggtacggtt accggcgtca acacagccgc gcagcgagta
       61 cagatcggtg agcacgacat cccttatgac tacctcgtgc tggcaaccgg cgcggcgcac
      121 agctactttg gtagggacga atggcagccc tatgcaccgg gactgaagcg gattgaagac
      181 gcgaccgcga tccgcggtcg cctgctgcga gcgttcgagc gcgcggaggc cacggaggat
      241 gatgaggagc gacgagcact gctgaccttt ctgatcgtcg gcgcaggtcc caccggtgtg
      301 gagctcgcgg gcgcgatcgt cgaactcgcg cgctttggaa tggaaaagga atttcgccgg
      361 tttgatccgg ccagcgcccg tgtgatcctg gtgcagtcgg gaccgcgcat cttgccagcc
      421 tttcatgagc gtctgtcggc gatcgcgcga cgatcgcttg agcgtatggg agtggaagtg
      481 ctgctggaca gccgcgtcga tcatatcgat gcctcgggtg tttcgatcgg aggtaaacga
      541 gtggctgccc gcaccgtgct gtgggctgcc ggcgtcaccg catcacccgc ggccaagtgg
      601 ttgcaggcgg ccgccgaccc cgcgggacgc gtcaaggtcc aggcggactt gagcattccc
      661 ggccacgaca acatattcgt catcggcgac accgcgctgt cggtagcgtg gaataaacaa
      721 cccgtgccag ggctcgcgcc cgcggccaaa caaggtggcg agtacgtcgc taaactcatc
      781 cgcgcacgcg tcagcggccg acggccacca gcgaatttcg tctatcggca cgctggcagt
      841 cttgccacca tcggccgaca ggcggccgtg gcggacttcg ggcgcatcaa gctgtggggg
      901 attctcgcct ggtggctttg gggcgtgatc cacatcgggt ttctgctggg cgtacgcaat
      961 cgcatggcaa cgatgatcaa ctggttctgg tcatacctga ccttcgccgg tggaatccgg
     1021 ttgattacgg gtgaggacga gacctagcgc gtggcttccc cttccgcgcc gacgagcgct
     1081 tggatctctt tctgtgacgg gcctgcttag gtcgagacgc cgctgttaga gtcctcgctc
     1141 gctcctgctc caacgccgca acgcgcgcat tctgatgttg cagtagtacc tgagtactct
     1201 tgagaacggt cagcaagctg gttcgctcgg ttcgcaaccc ctcgcgctct ttcgacagcg
     1261 aggccaggtg cgtaagttgc tcgccttcgc gagcaccggc ctcggcgagc gcgaccgaca
     1321 gcttcagtat ctgctcctgt agcgcggcgc tgtcaccgcc ggtgggctgg gcactcccct
     1381 gaagaatctt cagccgctcc cgcgcctctt cgattgcacg acgccacagc aactcggtga
     1441 cgcgggcaag gctctccggg acgcgctcga gcgcctcggg tcccgttacg agcctttgac
     1501 ccaaggtttc ccagtatttc gccaacagag gtgtcagcgt gttcggcgat ccgccaccca
     1561 gctgctcgcg cagcgccgcg actgacggct tgatgccttg gcgcaacagc gcatccgctg
     1621 ccctctgaac atccagtgca ttgatggcgc cgtctcgata gatcacatca cgaccgctga
     1681 aagctgcacg gtcgtgctgg ttgctggttg gttttggatc cttcattggg tacgcggctc
     1741 gcgcaccaag ggtagcggtt tttctccgct acttcacgac gccgctggct ttgaacgcag
     1801 cgatctgcgc gtcggaatac ccaaactcgc gcagcagggg atcagagtgc tcgccaacaa
     1861 ccggcgcacc tttcccggga aggaccgggg tcttggagaa gcggatcggg tgtcctgagg
     1921 ctttgtactt gccaagatcg cgattgttga gatcccagat caaccccaga gctgccgcct
     1981 gcgggtcggt gtacaggtcg gcgcgatagt tcaccggcgc gcacggcaca cccgcggctt
     2041 caaatttctg taaccaatag gcatttggct gtgtccgcaa acgcccctcg atgacgcgca
     2101 tcaatgactt ctgttggtag tactgctcgt cggaaaggtc caccggattg gtgtcgcaat
     2161 caggatcctc gagaccgagt gcgccgcaga ccttgcgacg ctgtcctcga tgtagcgcgg
     2221 caatactgat gtacccgtcg cgcgtctggt atacgcgata gtaggggacc atctgccgat
     2281 cggtgggacg atgcaggcag tcagggactt cgaccggtcg agcggtccgt ggcaggtcgg
     2341 ctcgcagccg tgagttggcg aaatcgtcga ctacttctgt gtgctttttg ccagccgcgt
     2401 aagcagtctt cagtgtctcg acttcgtgcc ggtgttgctc ggcgtcgaga ttcgcgattt
     2461 gaatcatctg ctgggcttgc agcgcgacac ccgtgctgaa aaggctggtc tcgatcttct
     2521 gcccctcccc cgactgggtg cgatggtaga gcgcgctgac gatgcccagc gcattgagca
     2581 tgccggtacc gatatc
//
LOCUS       DATMRM010000208         4149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3835528, whole genome shotgun sequence.
ACCESSION   DATMRM010000208 DATMRM010000000
VERSION     DATMRM010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3835528"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VNZ06_05990"
     CDS             complement(<1..754)
                     /locus_tag="VNZ06_05990"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWW20335.1"
                     /translation="MRLDRLSTFVIAVCLAVPAFAAKSDGNALPMAKPEGVEMSSERL
                     TELTKGMQQFVDQGRLAGVVTMVARHGKVVEFDAVGKRDIAANAPMQKDSIFRIYSMS
                     KPITGVAMMILFEEGKWQLNDPISKYIPEFKDLKVWSQGPNGNVVMKTPAHAPTMREL
                     MSHNAGFTYGIFSETPVDKLQRDADPLNINNTLDEFIKRMGKLPLNAEPGSEWHYSVS
                     VDIQGYLVQKLSGMPFDKFLEERVFKPLKMVDT"
     gene            complement(876..2234)
                     /locus_tag="VNZ06_05995"
     CDS             complement(876..2234)
                     /locus_tag="VNZ06_05995"
                     /inference="COORDINATES: protein motif:HMM:NF005460.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWW20336.1"
                     /translation="MVAPTLASLAADLAHGECTSRALVEQCLARIEDPKGEGKRAFLH
                     VERDSALAQADAMDALRSCGAAPSPYAGIPISIKDLFDIAGQVTRAGSIALNDRAPAE
                     RDAPCVARLRQAGFVFIGRTNMTEFAFSGLGLNPHYGTPLNPWNRSQRHIPGGSSSGA
                     GISVADAMAHGAIGTDSGGSCRIPAAFTGLVGYKPTARRIPRQGMIPLSSSLDSVGPI
                     ARSVSCCAVLDSLLANEPLPDLHDISLASLRFALPRTYVLDSLDRYVADDFHNAVSRI
                     AAGGARIDEIDIPELAEIPALNAKGSLSSAESYAWHRSMLATHSASYDPRVLSRIQAG
                     ATQSAADYIDLLEARARFIAQVNQRIADFDALIMPTVAVTPPRMTDLQFDGTYYRTNA
                     LVLRNCTVVNFLDGCAISLPMNAPGEPPTGFSLVCRQNTDRRLFRYATAVEHRLSSAE
                     AP"
     gene            complement(2228..3133)
                     /locus_tag="VNZ06_06000"
     CDS             complement(2228..3133)
                     /locus_tag="VNZ06_06000"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HWW20337.1"
                     /translation="MKTHFRRVTLALLSALFAATTSAATTANATSVLAGIDRSVVPGS
                     DFFGYANGAWLKNAQIPADLPSYGASEKLIELNEHRVVDLIRVAASKPSDASVETRQV
                     ADYYAAFMDEAAIEARGLAGLAPTMQRINAISDRTALARYLGTTLRADVDILNATNLY
                     SDNVFGLWVAQDLDDPSRYLPFLLQGGLGMPDRDYYLNDSPQMADDRSHYQAYIATVL
                     ELSGESDADQKAVKVLALERSIAQSWREKQRDAALRQQIVTDPHAPSQYRAVTCRNLD
                     AWYTAFHVTKGQSLYLPPMGRVRMW"
     gene            complement(3377..3910)
                     /locus_tag="VNZ06_06005"
     CDS             complement(3377..3910)
                     /locus_tag="VNZ06_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWW20338.1"
                     /translation="MKKDQAQAKAVSTLPTKSVVYEQLRDKLRDLIADQTDFLANISN
                     FAALLFTTLPELNWAGFYLLRGTDLVLGPFQGKPACSCIALGDGVCGTAALKRAAVLV
                     PDVHAFPGHIVCDEASQSEIVVPVFKDGHLVGVLDMDSPKIARFDEIDREQIEALLRI
                     LMEGTEVPGFRLAPPSP"
BASE COUNT          781 a         1254 c         1329 g          785 t
ORIGIN      
        1 cggtatccac catcttcagt ggcttgaaga cacgctcttc caggaatttg tcgaacggca
       61 tccccgagag tttctgcacc agatacccct ggatgtcgac cgatacgctg tagtgccact
      121 cgctgcccgg ctcggcgttg agcggcagtt ttcccatgcg tttgatgaac tcatcgagcg
      181 tattgttgat gttcagcgga tcggcgtcgc gctgcagctt gtcgacgggt gtctcgctga
      241 agattccgta ggtgaagccg gcgttgtgcg acatcagctc ccgcatggtc ggggcgtgtg
      301 ccggtgtttt catgaccaca ttgccgttgg ggccctggga ccagaccttc agatccttga
      361 actccgggat gtacttggag atcggatcat tcagttgcca cttgccttcc tcgaacagga
      421 tcatcatcgc gacgccggtg atcggcttgg acatcgagta gatgcgaaag atgctgtcct
      481 tctgcattgg ggcgttggcg gcgatatcgc gcttaccgac cgcgtcgaac tcgaccacct
      541 tgccgtggcg cgcgaccatc gtcacgacgc cggcaagccg gccctggtcg acaaactgct
      601 gcatgccctt ggtcagttcg gtcagccgtt cggaagacat ctccacacct tccggtttgg
      661 ccatgggaag cgcgttgccg tcggacttgg ccgcgaacgc gggcactgcc aggcagaccg
      721 caatgacaaa tgtactgagg cgatcaagac gcatgcgagg actccccctt gtcgattttg
      781 agattccagt cagccggaat tctagcgtgc cagcgagcgg ctccgcgagc gcgggcccca
      841 ttcaaggact tgtttgtaac aaattgtgag cgaggtcagg gggcttcggc actcgagagt
      901 cgatgttcga ccgcggtcgc gtaacgaaac agacgacggt cggtattctg gcggcagacg
      961 aggctgaacc cggtgggagg ctcgccgggc gcgttcatcg gcagcgagat ggcgcagcca
     1021 tccaggaagt tcacgaccgt gcagttgcgc agcaccaggg cgttggtgcg gtagtaggtg
     1081 ccatcgaatt gcaggtcggt catgcgcggt ggagtcaccg ccaccgtcgg catgatcagc
     1141 gcatcgaagt cggcgatgcg ttgattgacc tgtgcaatga aacgcgcgcg cgcttccagc
     1201 agatcaatgt agtcggccgc gctttgtgtg gcaccggcct ggatgcggct cagtacgcgc
     1261 ggatcatagg acgcgctatg cgttgccagc atcgagcgat gccaggcata gctctctgcc
     1321 gaggacagtg aacccttggc gttcagggcg ggtatctcgg ccaattcggg gatatcgatc
     1381 tcatcgattc gagcgccacc ggccgcgatt cgggaaacgg cgttgtgaaa atcgtccgcg
     1441 acgtagcgat ccaggctatc cagcacatag gtgcgcggca gcgcgaagcg cagactcgcg
     1501 agcgagatgt cgtgcagatc cggcaacggt tcattcgcca gcagcgaatc gagcactgcg
     1561 cagcaggaga ctgaacgcgc aatcggtccg acggaatcaa ggctgctgga gagcgggatc
     1621 atgccctgac gcggtatgcg ccgtgccgtg ggcttgtatc cgaccagtcc ggtaaatgcg
     1681 gctggaatcc tgcaggaacc gccgctatcc gtgccgatgg cgccgtgcgc catcgcgtca
     1741 gccactgaaa ttccggcgcc gctcgatgag cccccaggaa tgtggcgctg gctgcgattc
     1801 cagggattga gcggtgtgcc gtagtgcgga ttcagcccca gacccgagaa cgcaaactcg
     1861 gtcatgttgg tgcggccgat gaagacgaag cccgcctggc gcaggcgcgc aacgcagggc
     1921 gcatcgcgct cggcgggcgc ccggtcgttg agcgcgatcg agccggcgcg tgtcacctgt
     1981 ccggcaatgt cgaacagatc cttaatcgaa atcgggatgc cagcgtacgg cgagggcgca
     2041 gcgccgcagg agcgcagcgc gtccatcgca tcggcttggg cgagcgccga gtcgcgttcg
     2101 acgtggagaa aggctcgctt gccttcgcct ttcggatctt cgatgcgcgc caggcattgc
     2161 tcgaccagcg cccggctggt gcattcgcca tgggcgagat cggctgccag cgatgccagc
     2221 gtcggtgcta ccacatccgg acccgcccca tgggcggcag atacaggctt tgtcctttgg
     2281 tcacgtgaaa ggccgtgtac caggcatcga gatttcggca ggtgaccgcg cgatactggg
     2341 acggcgcgtg cggatccgtg acaatctgtt ggcgcagggc ggcgtcacgc tgcttctcgc
     2401 gccagctctg cgcgatgctg cgttcgagcg ccagaacctt gacggccttc tgatccgcat
     2461 cgctttcgcc gctgagctcc agtacggtcg cgatgtaggc ctgatagtga ctacgatcat
     2521 ccgccatctg tggcgagtcg ttcagatagt agtcacgatc tggcatcccg agtccgccct
     2581 gcaacagaaa gggcagatag cgactcggat cgtccaggtc ctgcgcgacc cacagaccga
     2641 acacattgtc gctgtagaga ttcgtggcat tgagaatgtc cacatcggcg cgcagcgtcg
     2701 tgcccagata gcgcgccagc gccgtccgat ccgagatcgc attgatccgc tgcattgtcg
     2761 gggcaagacc tgcgaggccg cgtgcctcga tcgcggcctc atccatgaag gcggcgtagt
     2821 agtccgcgac ctggcgcgtc tcgacactgg catcggatgg ctttgaggcg gcaacccgaa
     2881 tcaggtccac cacgcggtgc tcgttgagtt cgatcaactt ctcggaagca ccgtaggaag
     2941 gcagatccgc gggtatctgc gcgttcttga gccaggcgcc attggcgtag ccgaagaagt
     3001 cgcttccggg aacgaccgag cgatcaatgc cggcgagcac cgaggtggcg ttggcagtgg
     3061 tcgcggccga cgtggttgcg gcgaacagtg cgctcagcag tgccagcgtc acacggcgaa
     3121 agtgcgtttt catggtggac gagtataggt gtgtcatcgt ggctaccaca tgcgaacccg
     3181 ctcagcgggt gacaggtaga gggtctgccc cgacttgacg tcgaaggcgg cgtagcaggg
     3241 atcgagattt cgcgacatgg gcgcaggcca tcgccagcat ggccgcgagg acccgaaagg
     3301 gcagattcat agcgtgcctc gtgtggtacg aagccgctta gtataagact acgccgtccg
     3361 gcgcagactt taggtcttaa ggcgagggcg gcgccagccg gaagcccggc acttcggtgc
     3421 cttccatcag gatgcgcagc agggcttcga tctgctcgcg atcgatctcg tcgaatcggg
     3481 cgatcttcgg gctgtccatg tccagaacac ccaccaggtg gccgtccttg aacaccggta
     3541 cgacgatctc cgactgagag gcctcatcgc acacaatgtg gccgggaaag gcgtgcacat
     3601 cgggtaccag caccgcggcg cgcttcaggg ccgcggtgcc gcagacgccg tcgccgaggg
     3661 cgatgcacga acaggcgggc ttgccctgga acggtccgag caccagatcg gttccacgca
     3721 acaggtagaa tcctgcccag ttcaactctg gcagtgtcgt gaacagcagt gccgcgaagt
     3781 tagaaatgtt cgcgagaaaa tcggtctgat cggcgatgag atctcgcagc ttgtcacgca
     3841 gctgctcgta gacgaccgac ttggtcggca gggtgctgac cgcttttgcc tgcgcctgat
     3901 cttttttcat gcgcacatag tatgagctaa acagccgaac cgtcgcgcga caactgcgtc
     3961 aactttccgc aagctgacgc agttgccata tccaacgccc tatcgggccc agtggccctc
     4021 ttcgagatgc cagccaccac gcgatccctg aacccagcgg tgcgcgaccc aatgatagcc
     4081 cgctctgccc gcgccccagt ggccgcccac ccacacatgg cgtccgccga cccagttcca
     4141 gtagcccgg
//
LOCUS       DATMRM010000209        11526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3515957, whole genome shotgun sequence.
ACCESSION   DATMRM010000209 DATMRM010000000
VERSION     DATMRM010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3515957"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..3622
                     /locus_tag="VNZ06_06010"
     CDS             170..3622
                     /locus_tag="VNZ06_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWW20339.1"
                     /translation="MSARRPHRRSSPAWAALLGVALSCGAQAAAPAPPPGQTIGDLDT
                     KAVPVQRDSKGPVGGAAKAMENYRQFLQLQNADPKLRAEALRRLGDLNLESGELERMA
                     SEVTLVDRQGAEAIRLYTTLLKGYPNYPRNDQVLYQLARAYETTGQSDLALSTLDSVV
                     ARYPQSRDIAEVQFRRGEILFSNKRYPDAQHAYEAVLAKGRFGSAFYAQSLYKHGWSL
                     FKQGQNEDSLKSFGDLLDVTLMDPTDNTRMRKLDSLGRADRELVDDTMRVMSITFSYL
                     DGAKSLDEFSAHRSAMPYAYMLYQRLGDLYVEKQRFQDAATTYRAFVSRDPVDEHAPL
                     LTNAAIEAYRKGGFAELMLDGKLEYVQRYGFDAPFWNNRQHGGYPQVVADLKTNLKDV
                     AEYYHASAQKSKKAEDYSSAAHWYRAYLSSFPGDPDSAATDYLLADALFESKQYQDAA
                     AEYERTAYQYPKNAKSAEAGYAALVAYQKQEDGLQPEVRATVHKQATASGVKFAQTFP
                     DHPQAAVVLTRAAQDLYASGDQTQAVQTARALLARTPPVDNAKQRIGWTIVGQVAFNQ
                     GDFPAAEDAFGHALAVAAPNDPERSDITERLADSVYKQGEAKRNAGDQSGAAADFLRV
                     ARVAPASKVIATSQYDGAAALINAQQWDKAIDVLEAYQRDYPKSQYAADVSHKLATAY
                     VAAGRSAQAAAAFEKIASNPKEDPAVVHEATAQAADLYAQSGNSARAIPLLERLVKDY
                     PTPAADAIEVRQRLLEIAAKNGEADRVRYWQREIVKADSAAAAGRTERTKYLAAKAQL
                     ALSTPARDEFRSIKLVAPLKQSLAAKRKALENAVQVYKEVAAYQVAETTTAATYETAE
                     LYHTLAQDLMKSERPKKMSPEELEQYNSLLEEQAFPFEEQSISIHELNTKRALDGVYD
                     ESVRKSFAALAELKPARYGKTELSAGMMGPAAQPGPPPVPPPSAAVQADFQRILDLAN
                     AGKNTDAELELKQFQLQHAGYAAPAIDLGLLTRQEGKLSDSEASLRYATALDSTSAVA
                     WDELGITLRSEGKFADARAAYQRAIAANDSYAPAHRNMGVLLDLYIGDPAAAIPEFER
                     YKALTSEDKPVGTWIAELRARTGIKAPAPAQPSAPATPDNAAPGAPAPAPSSTAAGNN
                     GGPT"
     gene            3619..4137
                     /locus_tag="VNZ06_06015"
     CDS             3619..4137
                     /locus_tag="VNZ06_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20340.1"
                     /translation="MRRHLQTSAIAALGIVLTAGGALAQEIEPGVQTSSAAAPVPKAS
                     PPANPAAAAPKPKAAPAPARTAPPNAAPTADGRTKSSDRIELDTTQISGNRELPKVMY
                     VVPWRRADPGDFAGRPPNSLLDEALTPVDRDVFRRQNSYYGAVRDQTQVPAAQSGAAA
                     TSGSAATPKDEK"
     gene            4221..4871
                     /locus_tag="VNZ06_06020"
     CDS             4221..4871
                     /locus_tag="VNZ06_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWW20341.1"
                     /translation="MDIFSHVVRFFQGGGFMMYFIAAVLVVGLAIAIERWVYLTVTAL
                     RNRGLWNEISPFLAQGNFRQAVQLTSKSPAAIGQILSYGLARIASARRREDIEKAMEE
                     SLMDIMPRLERRTHYIATFANLATLLGLLGTVIGLINAFAAVATVNPAEKANLLSASI
                     SVAMNCTAFGLGTAVPLLLIHAVLQTKTTELIDSLEMASVKFLNAVTERQPQPAAA"
     gene            4917..5414
                     /locus_tag="VNZ06_06025"
     CDS             4917..5414
                     /locus_tag="VNZ06_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWW20342.1"
                     /translation="MQRHQRKPQELLLVSMIDIFTVLVTFLLMTAVFSRTVILELKLP
                     PANADFTPPPPGLQLEVVVRKDLLLVDDRNSGPLATFPNKDGNYDFDALSEYLQRVKS
                     KYPDKTDATILLEPDTPYDIVIQTMDRIRVLEVNAGLSVEQYELFPDISIGDAPVLDA
                     AAPAH"
     gene            5427..5951
                     /locus_tag="VNZ06_06030"
     CDS             5427..5951
                     /locus_tag="VNZ06_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWW20343.1"
                     /translation="MSMSNRARRMMQHQLRHRADAELNLIPMIDILSVMVSFLLVYST
                     NVEVLQNNKAIEIPQSIAEQQPKQTVVVMLTRDQLFVQGELIESVAEIHANNGSILAP
                     LRAALKRPTLVGQEVTEKDLADREITVMADKSLPYEIIKKVMATCTDADYGKLSFAVL
                     QKDKPVSANALRPS"
     gene            5965..6873
                     /locus_tag="VNZ06_06035"
     CDS             5965..6873
                     /locus_tag="VNZ06_06035"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HWW20344.1"
                     /translation="MQLTPYYRSFDLAWEQDPEEQGRLRKLVTAGLLLLLLLGLIIPF
                     IHLPKATEAPEAVPERLARLMVQEKPKPPPPPPVELPKPKPETKPVEVPKPVDKVAEA
                     RKKAEKSINKLKDELADLRQNVDLTPLGQTKNLQGAVGQDSHAERSLIASKAGSGSAG
                     ITSANASRGFGTGAGSLTGHDTTSVNSRIAAAGSPGNVSRGVSGKPSRSEEEIALVFD
                     RNKGAIYALYGRALRDTPDLQGKLVLEFTISPSGEVTECHVVSSELKDPDLERKIVAR
                     VKLFRFEAKDVASITTTKPIEFVPST"
     gene            6883..7242
                     /locus_tag="VNZ06_06040"
     CDS             6883..7242
                     /locus_tag="VNZ06_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20345.1"
                     /translation="MNLQSEIQYFSELQPMDQARLMAVFLHELTVEARTTYGTSLEHV
                     EDPARLRFVNEIVCRLARFTEQLLADDRNRPADDVVIRMLLSPRADKPAERIVQNAYR
                     RAIHGFDRYDTTVAMDK"
     gene            7295..9211
                     /locus_tag="VNZ06_06045"
     CDS             7295..9211
                     /locus_tag="VNZ06_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HWW20346.1"
                     /translation="MILLLASVLVVALARRIGLPAILGYLIVGIAVGPHALALVSNGS
                     QTQAIAEIGVVFLLFTLGLEFSFPRMLAMKREVMALGAAQTLIVGGAGALVTHLIDVR
                     WPSAIVAGGALAMCSTAIVLQQLGDQDELNRSHGRLSFAVLLFQDLAFVPLLTLATVQ
                     TQAVSASGTMLVVRLVGLGMIGLAAVLALGRWLVRPLLYEIAHSRLRELFTLSALLIV
                     LTSAWITQRMGLSMALGAFLAGMLLAETEYRHQIDATIRPFRELLLGLFFISVGMLLD
                     LRVLAVDFISIVGLLLALLVSKTLLTAITTFAFGADRFKSMRTGLVLAGGGEFGVALL
                     TILARGDDLVPVRPAQLLLSVLVLSMIVSPVIIRYNKRIARFILRERGPPVTAIEREA
                     AATKALAGREHVILCGFGRVGGHLGRVLDTQGFEYLAVDRDPAKVRAARQGGLPVVWG
                     DCADEELLNNVGLDRANVVIVTFADPMIALEIVRAVRRLRSDVPVLVRTQDESRLTEL
                     SEAGATEVVPETLEASLTLVSQALTLLNMPATQVAHTIDEMRRRRYATLRTPGWTEEL
                     RSIVLPPGGWAVGRRLEEIRARGAEVAFTAVRRQGITGREPDGETELREGDVVVIFGT
                     PEALEHAEAVLLAG"
     gene            9285..>11526
                     /locus_tag="VNZ06_06050"
     CDS             9285..>11526
                     /locus_tag="VNZ06_06050"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HWW20347.1"
                     /translation="MNAANIPFPTRHHPLRDDIQTLAELTDEVLREQGGEELWQLVEQ
                     DRVTAVRWRDAVPGAAEALAVRVRGRPPRIARELVRAFSAWFQLVNVAEKVHRIRRRR
                     EYFQQDGDRPQPGGVEDALSELKSAGLSLSEILTLLQQLSIEPVLLAHPMESTRRTNL
                     RRQQRMASLLLQRDNVSLAPNERRLLLERIRAEVSADWQTEEHPRDRLTIADEREHAV
                     FYLAEILYRIVPAFYEEIGTALGQLYGVSPETVELPVIVRFGTWVGGDMESSSEVHAK
                     SIRETLARAQQIIIKSYYDECLKLSQSLSQSANRIGISAAVTRRVEEYRTLLPGAQGL
                     TPSRHDRMPYRILLGQIAERLHATYDGRSTGYQQPSQFRADVALVAHSLLANRGTHAG
                     YYHVRRLLYRIDTFGFHLATLDLRAPAALHHAVLAQGLDDPEWPKLSAAERHARLVGI
                     LERDTGPTGTFDALAKRTLAVFDAIIQSRHRYGSEAVGLYVVGGASQADDVLAPLVLA
                     RWAGAYDKGTGQVALDIAPQFDTVESLEECGVVMRELLQDGVYKQHLEGREKLQTVLI
                     GYSDSNQQCGIVASRFAAFRAQRSLTEALKQAQKQHVLFYSRGGSTARGGGRIDALLR
                     AAPAESVNGILRFTEQGESISHSYGLLPNAMRTLERAFNTLAQATLAVRRKVAVREGA
                     ALSECVGLAAGHSAQVWRGLVHEQPQFYDFFRAVTPIDVIERMQIGAQQSVRVERRGV
                     DSTRPAL"
BASE COUNT         2126 a         3632 c         3752 g         2016 t
ORIGIN      
        1 accgcctgac cacgtactcg ctgcaggcgc gggtccagct ggccgatatt tacgatcgct
       61 cttcggacag cgagaagccg tccgccgcgc cggccacacc gggcaatgag cccacgcccg
      121 aacagaaatc cgttccagag ccggcgaacg caccggcacc tgagccgaaa tgagcgcgcg
      181 ccgccctcat cgccgctcgt cccctgcatg ggctgcgctg ctgggcgtgg cgttgtcgtg
      241 cggcgcgcag gcggcagctc ctgcgccgcc cccggggcag accatcgggg atctggacac
      301 caaggcggtt ccggtccagc gcgacagcaa gggtccggtc ggcggggctg ccaaagcgat
      361 ggagaactac cggcagttcc tgcagttgca gaatgccgat cccaaacttc gcgccgaagc
      421 gctgcgccgg ctcggcgatc tgaacctgga gtcgggcgaa ctcgagcgca tggcgagcga
      481 agtgaccctg gtcgatcgtc agggcgccga ggcgatccgt ctctatacga cgctgctgaa
      541 gggctatccg aactatccgc gcaacgatca ggtgctctat cagctggcgc gggcctatga
      601 gaccacgggc cagagcgatc tggccctcag tacgctcgac agtgtggtcg cccgctaccc
      661 gcagagtcgt gacatcgccg aggtgcagtt ccggcgaggc gagatcctgt tctccaacaa
      721 acgctatccc gatgcgcagc atgcctatga ggcggtactg gcgaagggac gtttcggctc
      781 cgcgttctat gcgcagagcc tctacaagca cggctggtcg ctgttcaagc agggccagaa
      841 cgaagacagt ctgaagtcct tcggcgacct gctcgacgtg acgctgatgg atccgactga
      901 caacacgcgc atgcgcaagc tggattcgct cggccgcgcc gatcgcgaac tggtggacga
      961 tacgatgcgg gtgatgagca tcacgttctc gtacctggac ggggccaaat cgctcgacga
     1021 gttcagtgcg catcgcagcg cgatgccgta tgcgtacatg ctctatcagc gcctgggcga
     1081 tctgtacgtc gagaagcaac gcttccagga tgcagccacg acctatcgcg ccttcgtcag
     1141 tcgcgatccg gtggacgaac atgcgccgct gctgaccaat gctgcgatcg aggcctaccg
     1201 caagggtgga ttcgccgagc tgatgctgga cggcaaactc gagtacgttc agcgctacgg
     1261 tttcgatgcg ccgttctgga acaaccgcca gcacggcggc tacccgcagg tggtggccga
     1321 tctgaagacc aatctgaaag atgtggccga gtactatcat gccagcgcgc agaagagcaa
     1381 aaaggcagag gattattcgt ccgcggcgca ctggtatcgg gcttacctga gttcgtttcc
     1441 gggcgatccg gattcggccg ccaccgacta cctgctggcc gacgcgctgt ttgaaagcaa
     1501 gcaatatcag gatgccgccg ccgaatacga gcgcaccgcg taccagtatc cgaagaacgc
     1561 gaagtcagcc gaagccggct acgccgctct ggtggcctat cagaagcagg aggacggtct
     1621 gcaaccggag gtgcgcgcca cggtgcacaa gcaggcaacc gcttccggtg tcaaatttgc
     1681 gcagaccttt cccgatcacc cgcaagccgc ggtcgtgctg acccgcgcgg cgcaggatct
     1741 gtacgcctcc ggcgatcaga ctcaggccgt gcagacggcg cgcgcactgc tggcgcgaac
     1801 cccgcccgtg gacaacgcga aacagcgtat cggctggacc attgtgggcc aggtggcctt
     1861 caatcagggc gactttcccg ccgccgagga tgcctttggg cacgcactgg cggtagcggc
     1921 gccgaacgat cctgagcgtt cggatatcac cgagcgcctg gcggattcgg tctacaagca
     1981 gggcgaagcc aagcgcaacg ccggcgatca atccggtgcg gcagccgatt tcctgcgtgt
     2041 cgcacgggtg gctccggcct ccaaggtgat cgcgacctcg cagtacgatg gcgccgcggc
     2101 gctcatcaat gcgcaacagt gggataaagc gatcgatgtg ctggaagcct atcagcgcga
     2161 ctatccgaag agtcagtatg ccgcggacgt gagccacaag ctggccaccg cctacgtggc
     2221 cgcgggccgc tcagcacagg ccgccgcagc gttcgagaag atcgcctcca atccaaagga
     2281 agatccggcg gtggtgcatg aggcaacagc ccaggcggct gacctgtacg cgcagtcggg
     2341 aaacagtgcg cgcgcgattc cgctgcttga gcggttggtg aaggactatc cgacaccggc
     2401 cgcggacgcg atcgaagtgc gccagcgtct gctggagatc gctgccaaga atggcgaggc
     2461 ggaccgagtg cgctactggc agcgcgagat cgtcaaggcc gattcggccg cggcagccgg
     2521 gcgaaccgag cggaccaaat atctggccgc gaaggcgcag ctggcgctga gcacgccggc
     2581 gcgcgatgag ttccgcagca tcaagctggt cgcgccactc aaacaatcgc tggccgccaa
     2641 gcgcaaggcg ctggagaacg cggtgcaggt ctacaaggag gtcgccgcct atcaggtggc
     2701 cgagaccacc accgcggcaa cctacgagac cgccgagctg tatcacacgc tggcgcagga
     2761 cctgatgaag tcggaacggc caaagaagat gtcgcccgag gagctcgagc aatacaactc
     2821 gctgctcgag gagcaggctt ttccgttcga ggaacagtcg atctcgatcc acgagctcaa
     2881 caccaagcgc gcgctggatg gggtctacga tgaatcggtg cgcaagagct tcgcggcgct
     2941 cgccgaactc aagcctgcgc gctacggcaa gaccgagctc agtgccggca tgatggggcc
     3001 agcggcacag ccggggcctc ctccggtacc gccgccgagt gctgccgtgc aggccgattt
     3061 tcagcgcatt ctcgatctgg ccaatgccgg caagaacacc gatgccgagc tggagttgaa
     3121 gcaattccag ctgcagcacg ccggctatgc cgcaccggcg atcgatctgg gattgctcac
     3181 ccggcaggaa ggcaagctga gcgattccga agcgtcgctg cgctatgcca ctgcacttga
     3241 cagcaccagt gcggtcgcct gggatgaact ggggatcacg ctgcgcagcg agggcaagtt
     3301 cgccgatgcg cgtgcggcct atcagcgcgc gatcgccgca aatgatagtt acgcgccggc
     3361 acatcgcaac atgggcgtat tgctggacct gtatattggc gacccagccg ccgcgatccc
     3421 ggagttcgaa cgctacaagg cgctgacttc agaggacaag ccggtcggga cctggatagc
     3481 ggaattgcgc gcccgtaccg gcatcaaagc accggctccc gcgcaaccct cggcgccagc
     3541 gacgccggac aacgccgcgc ccggcgcgcc ggctcccgcg ccgagtagca cagcggccgg
     3601 taacaacgga ggcccgacat gagacgccac ctgcagacat ctgccatcgc agccttaggg
     3661 atcgtgctga cggccggcgg cgcgctggcg caggagattg agcccggcgt gcagacttcg
     3721 agcgctgccg ccccggtgcc gaaggcttcg ccgcctgcca atcccgcggc ggccgctccc
     3781 aagccgaagg ccgcacccgc acctgccagg accgcgccgc ccaatgcggc cccgacggcc
     3841 gacggccgca ccaagtcctc cgatcgcatc gaactggaca ccacgcagat cagcggcaac
     3901 cgcgagctgc cgaaggtcat gtatgtcgtt ccctggcgac gggccgatcc gggcgatttt
     3961 gccggtcggc cgcccaacag tctgctggat gaagcactga cgccggtgga tcgcgatgtt
     4021 tttcggcgac agaatagtta ttacggagcg gtgcgggacc agacgcaggt accggcggct
     4081 caatccggag ccgccgcaac ctctggaagc gcggcaaccc ccaaggatga gaaataggtc
     4141 gcggtttgag cttcgacctc actttagttt gagcgttctg aatcgaaccc tgagcgggaa
     4201 ggcattggga gtgaagcacg atggatattt tcagtcacgt agtcaggttc ttccaaggcg
     4261 gcggattcat gatgtacttc atcgccgcag tcctggtggt cggtctggca atcgcaatcg
     4321 agcgttgggt ctacctgacg gtcaccgcat tacgcaaccg cggtctgtgg aatgagatct
     4381 cgccgtttct ggcgcagggc aatttccgcc aggcggtgca gctcacctcc aagtccccgg
     4441 ccgcgatcgg ccagattctg agctacgggc tggcacgcat cgcgtcagca cgtcgccgcg
     4501 aggacatcga gaaggcgatg gaagagagcc tgatggacat catgccgcgc ctggagcgcc
     4561 gcacgcacta catcgcgacc ttcgcgaacc tggcgaccct gctgggtctg ctgggtaccg
     4621 tcatcggcct catcaacgcc ttcgccgcgg tcgcgaccgt caatccggcc gagaaggcga
     4681 acctgctgtc cgccagcatc tcggtggcga tgaactgtac cgccttcggt ctggggaccg
     4741 cggtgccgct gctgctgatt catgcggtgc tgcagaccaa gacgaccgaa ctgatcgaca
     4801 gcctggaaat ggcctccgtg aagttcctga acgccgtgac cgagcggcag ccgcagcctg
     4861 cggcggcctg actcgaggtt tgatgcgccg tgcgccgctc ctatagcgtc cgaaagatgc
     4921 agcgccacca gcgtaagccg caggagcttt tgctggtgtc gatgatcgac attttcaccg
     4981 tactcgtgac cttcctgctc atgaccgctg tgttctcgcg caccgtgatc ctggagctga
     5041 aacttccgcc tgccaatgct gacttcacgc cgccgccacc gggactgcag ctggaagtgg
     5101 tcgtgcgcaa ggatctgttg ctggtggacg atcgcaactc ggggccgctc gcgaccttcc
     5161 cgaacaagga cggtaactac gacttcgatg cgctatcgga atacctgcag cgcgtgaagt
     5221 cgaagtaccc ggacaagacc gatgccacga tcctgctcga gccggacact ccctatgaca
     5281 tcgtgattca gacgatggac cgcatccgag tgctggaagt caacgcgggc ttgagcgtgg
     5341 agcagtatga gctgttccct gatatttcga tcggcgatgc gcccgtgctg gacgccgcgg
     5401 cgccagcaca ctgacgggag tcaatcatga gcatgtccaa tcgcgcccga cgcatgatgc
     5461 agcaccagct gcgacatcgt gccgacgccg agttgaacct gatcccgatg atcgacatcc
     5521 tgagtgtgat ggtgtcgttt ctgctggtgt attccaccaa tgtcgaggtc ttgcagaaca
     5581 acaaggccat cgagattcca caatcgatcg ccgagcagca gccaaaacag accgtggtcg
     5641 tgatgctgac cagggatcaa ctgttcgtgc aaggtgaatt gatcgagagc gtggccgaaa
     5701 ttcatgccaa caacggcagt attctggcgc cgctgcgcgc cgcactgaag cggcccacgc
     5761 tggtgggtca ggaagtgacc gagaaggatc tggctgatcg ggaaatcaca gtgatggcgg
     5821 acaagtcgct gccctacgag atcatcaaga aggtcatggc cacctgcacc gacgccgact
     5881 acggcaagct gtccttcgcc gtgctgcaga aggacaagcc cgtgtccgcc aatgcgttac
     5941 ggccatcgtg acgcggaatc acccatgcaa ctgactccgt actaccgcag tttcgatctg
     6001 gcctgggagc aggacccgga ggaacagggc cggctgcgca aactcgttac cgccggcctg
     6061 ctgctgctac tgctgctggg tctgatcatt ccgttcattc atcttcctaa ggccacggag
     6121 gcgcccgagg cggtgcctga gcgcctggcg cggctgatgg ttcaggagaa gcccaaaccg
     6181 ccgccgcccc ctccggtgga gttaccgaag ccaaagccgg agaccaagcc ggtggaggtg
     6241 cccaagccgg tggataaggt cgccgaggca cgcaagaagg ccgagaagtc catcaacaag
     6301 ctcaaggacg agctggcgga tctgcgtcag aacgtcgatc tgacccccct cgggcagacc
     6361 aagaatctgc agggtgcggt gggccaggac agccacgccg agcgctcgct gattgcatcg
     6421 aaagcgggca gcggctcggc cggtattacc agcgccaatg cgagccgcgg cttcggcacc
     6481 ggtgccgggt cgctgaccgg acatgacacg acctccgtca attcccgcat tgcggccgcc
     6541 ggctcgccag gcaatgtttc gaggggtgtc agcggcaagc cctcgcgcag cgaggaggag
     6601 atcgcgttgg tgttcgatcg caacaaaggt gcaatctatg cgttgtacgg tcgggctctg
     6661 cgtgacacgc ccgatctgca gggcaaactt gtgctggagt tcacgatttc gccttcgggc
     6721 gaagtgacag aatgccatgt cgtctcgagc gaattaaagg atcctgacct cgagcgcaag
     6781 atcgtggcgc gcgtgaaact gtttcgcttc gaggccaagg acgttgcaag cattaccacc
     6841 accaagccga tcgagttcgt accctcgacg tagagtcgcg acgtgaacct gcagtctgaa
     6901 attcaatact tcagcgagct gcagccaatg gatcaggcaa gactcatggc agtgttcctg
     6961 catgagttga cggtcgaggc tcgtacgacc tacggcacca gtctcgagca cgtcgaggac
     7021 ccggcgcgtc tgcgcttcgt caatgaaatc gtctgccgtc tggcgcgctt caccgaacaa
     7081 ttgctggcgg acgatcgcaa tcgtcccgcc gatgacgtcg tcatccgcat gctgctgtcg
     7141 ccgcgtgcgg ataaacctgc cgagcgcatc gtgcagaatg cctaccggcg cgcgatccac
     7201 ggcttcgatc gctacgacac cacggtggca atggacaagt gacgcagcgg cgccgtaacg
     7261 tggtgctgtg ccgttacagt ctctaagtca ggtcttgatt ctgttactgg cctcggtgct
     7321 ggtcgtggca ttggcacgac gcatcggcct gcccgccatt ctcggctatc tgatcgtcgg
     7381 catcgcggtc ggtccacatg cactcgccct ggtctccaac ggcagccaga cacaggcgat
     7441 cgccgaaatc ggcgtggtgt ttctgctgtt cacgctcggg ctggaattct cgtttccccg
     7501 catgctggcg atgaagcgcg aggtcatggc cctgggtgcg gcacagacgc tgatcgttgg
     7561 cggcgccggc gcgctggtca cccacctcat cgatgtgcgc tggccgagtg ccatcgttgc
     7621 cggcggtgcg ctggccatgt gctccaccgc gatcgttctg cagcagctgg gtgatcagga
     7681 cgaactcaac cgcagtcatg gccgactgtc gttcgcggtg ctgttgtttc aggatctggc
     7741 gttcgtgccg ctgctgacgc tggcgacggt gcagacgcag gcggtctcgg cctccggcac
     7801 gatgctggtc gtgcggctcg tgggactggg tatgatcggt ctcgccgcgg tactcgccct
     7861 cgggcgctgg ctggttcggc cgctgctcta cgaaatcgcg catagccgtc tgcgcgagct
     7921 gttcacgctg tcggcgctgc tgatcgtgct gacgtccgcc tggatcacgc agcgaatggg
     7981 cctgtcgatg gcactcggtg cgtttctggc cgggatgctg ctggcggaga ccgagtatcg
     8041 acatcagatc gatgccacga tccgcccgtt ccgcgagctg ctgctgggtc tgttcttcat
     8101 ctcggtcggc atgttgctcg acctgcgggt tctggccgtg gatttcatca gcatcgtcgg
     8161 attgctgctg gcgctgctgg tcagtaagac gctgctcacc gccatcacca cattcgcgtt
     8221 cggcgccgat cgcttcaagt cgatgcgcac gggtctggtg ctggccggcg gcggcgaatt
     8281 cggcgtggcg ctgctcacca ttctggcgcg cggcgacgat ctggtgccgg ttcgcccggc
     8341 gcagctgctg ctgtccgtgc tggtgctgag catgatcgtg agtccggtca tcatccgcta
     8401 caacaaacgc atcgcgcgct tcatcctgcg cgaacgcggt ccgccggtca ccgcgatcga
     8461 gcgcgaagca gcggccacca aagcgctggc aggacgagaa cacgtgatcc tgtgcggttt
     8521 cggccgcgtc ggtgggcatc tcgggcgggt actggataca cagggtttcg aataccttgc
     8581 ggtggatcgg gatccggcca aagtacgcgc cgcccgtcag ggtggcctgc cggtcgtctg
     8641 gggtgactgc gcggacgagg aactgctcaa caacgtcggc ctcgatcgcg ccaatgtggt
     8701 cattgtgacc ttcgccgatc cgatgattgc gctggaaatc gtgcgcgcgg tgcgcagact
     8761 gcgctccgat gtgccagtgc tggtgcgcac ccaggacgag tcgcgcctga cagaattgtc
     8821 ggaggcggga gccaccgagg tggtacccga gaccctggaa gcgagcctga ccctggtatc
     8881 ccaggcgctg acgctgctga atatgcccgc gacgcaggtc gctcatacca tcgatgaaat
     8941 gcgccgccgc cgctatgcca ccttgcgcac tcccgggtgg acagaggagc tgcgctcgat
     9001 cgttctgccg cctggaggat gggcggtggg gcggcgtctg gaggaaatcc gggcccgggg
     9061 cgcggaagtg gcctttaccg ccgtgcggcg tcagggcatc accggacgcg agccggacgg
     9121 cgaaaccgaa ttgcgggaag gggacgtcgt ggtgattttt ggtaccccgg aagccctcga
     9181 gcacgcggag gccgtactgt tggccggtta ggagaagcgc ctgttcagcg ccacgggacc
     9241 gctcagacag cggccccagg aagtcgctag aattcgtacc gacgatgaat gccgccaata
     9301 ttccgttccc gacgcgccac catccgttgc gcgatgacat ccagacgctg gccgagctca
     9361 ccgacgaggt gctgcgcgag caaggtggcg aggagctgtg gcagctggtc gagcaggatc
     9421 gcgtcacggc ggtgcgttgg cgcgacgctg ttccgggcgc cgccgaggcg ctggcagtgc
     9481 gggtccgggg ccggccgccg cgcatcgcgc gcgagctggt acgggcattt tccgcctggt
     9541 tccagttggt caacgtcgcc gagaaggtgc accgcatccg tcgccggcgt gaatactttc
     9601 agcaggacgg cgaccgtccg cagcccggag gcgtcgagga tgcgctctcg gagctgaagt
     9661 cggcgggctt gagcctgtcg gaaattctaa ccctgctgca gcagctctcg atcgagccgg
     9721 tgctgctggc gcatcccatg gaatccaccc gccgcaccaa tctgcggcgt cagcagcgca
     9781 tggcatcgct gctgcttcag cgcgataacg tcagcctggc gccgaacgaa cggcgcctgc
     9841 tgctcgaacg catccgcgcc gaggtcagcg ccgactggca gaccgaagag cacccgcgcg
     9901 atcgcctgac gatcgccgat gagcgcgagc acgcggtctt ctatctggcg gagatcctct
     9961 atcgcattgt gccggcattc tacgaggaga tcggaaccgc tctgggccag ctctatgggg
    10021 tatcgcccga gacggtcgag ttgccggtga tcgtgcgctt tggcacctgg gtgggcggtg
    10081 atatggagtc ctcgtccgag gttcacgcca agagcatccg cgaaaccctg gcgcgcgcgc
    10141 agcagatcat catcaagtct tactatgacg aatgcctgaa actgtcgcag tcgctgtcac
    10201 agagcgccaa ccgcatcggc atctcggccg cggtcacacg acgggtcgag gaataccgca
    10261 cgctgctgcc gggggctcag ggcctgacgc cctcgcgcca cgatcgcatg ccgtatcgca
    10321 ttctgctggg gcagatcgcc gagcggctgc atgctaccta cgatggacgc tcgaccggct
    10381 atcagcagcc atcgcagttt cgggccgacg tggcgctggt ggcgcacagt ctgctcgcca
    10441 accgcggcac gcatgccggt tattaccatg tgcggcgctt gctctaccgc attgacacct
    10501 tcgggtttca tctggcgaca ctggatctgc gggcgccggc agcgctacac cacgcggtcc
    10561 tggcgcaggg cctggatgat ccggagtggc caaaattgag cgcagccgag cggcatgcgc
    10621 gactggtcgg cattctggag cgcgacaccg gtcccaccgg cacgtttgat gcgctcgcca
    10681 agcgaaccct ggcggtgttc gacgccatca ttcagagccg tcatcgctac ggttccgagg
    10741 cggtcggtct gtatgtcgtc ggcggcgctt cccaggccga tgacgtgctg gcgccgctgg
    10801 tcctggcacg atgggccggc gcctatgaca aaggcaccgg tcaggtggca ctcgacatcg
    10861 cgccgcagtt cgacacggtc gagtcgcttg aggagtgcgg cgtcgtgatg cgcgagctgc
    10921 tgcaggatgg tgtctacaag cagcacctgg aaggacgcga aaagctgcag accgtgctga
    10981 ttggttactc cgacagcaat cagcaatgtg gcatcgtggc ctcgcgtttc gccgcatttc
    11041 gggcccagcg ctccctgacc gaggcgctga agcaggcgca gaagcagcat gtactgttct
    11101 atagccgtgg cggcagcacg gcacgcggtg gcgggcgcat cgatgcactg ctgcgcgcgg
    11161 cgcccgccga atcggtcaac ggcatcctgc gtttcaccga gcagggcgag agcatcagcc
    11221 acagctacgg cctgctgccc aatgcgatgc gcacgctcga gcgcgctttc aacacgctgg
    11281 cgcaggcgac gctggccgtg cgccgcaagg tcgcggttcg ggaaggtgct gcactgagtg
    11341 agtgcgtggg tctcgccgcc ggccatagcg cacaggtctg gcgtggcctg gtgcatgagc
    11401 agcctcagtt ctacgacttc ttccgtgcgg tgacgccgat cgatgtgatc gagcgcatgc
    11461 agatcggggc ccagcagagc gtgcgtgttg agcggcgggg ggtcgactcg acccgaccgg
    11521 cgctgt
//
LOCUS       DATMRM010000210         7360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2601690, whole genome shotgun sequence.
ACCESSION   DATMRM010000210 DATMRM010000000
VERSION     DATMRM010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2601690"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /gene="glyA"
                     /locus_tag="VNZ06_06055"
     CDS             complement(<1..1068)
                     /gene="glyA"
                     /locus_tag="VNZ06_06055"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWW20348.1"
                     /translation="MLSSRMTISEFDPMLQRSLDGERRRQEDHIELIASENYASPRVL
                     EAQGSVLTNKYAEGYPGKRYYGGCEFVDIAEQLAIDRARKLLGADYVNVQPHSGSQAN
                     AAVYLSLLTPGDVILGMSLDHGGHLTHGAKVNFSGKLFHAHQYGVHPETGLMDYGQIE
                     RLTLEHRPKMLVAGFSAYSRVIDWARLRSIADVVGALLFVDMAHVAGLVAAGEYPNPL
                     PHADVVTTTTHKTLRGPRGGMILARANEAITKKINSMVFPGTQGGPLMHVIAAKAVAL
                     LEALQPEFKQYQRQVVANARAMCAVLVERGYHIVSGGTDNHLFLLSLVGRPITGKEAD
                     AALGKAHITVNKNAVPNDPRPP"
     gene            complement(1138..2094)
                     /locus_tag="VNZ06_06060"
     CDS             complement(1138..2094)
                     /locus_tag="VNZ06_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20349.1"
                     /translation="MNAIGSEVAVVIGERLARYGFGDGHPFGPDRHAAFLREFEQRGL
                     PRQVVTLEPRDATSAELQLFHTAEYVHFIMERSQSGIGYLDGGDTPAYRGVFEAASCV
                     VGSTLTATDWIMRGTRRRGFVPIAGLHHAARNRAAGFCVFNDCGVAVEHLRRTYGMAR
                     IAYIDIDAHHGDGVFYGFESDPDLIFADLHESGESLYPGTGSDAETGRGAAEGTKLNV
                     PLPAGSGIVEFEQVWPQMLAHVERFSPAFFILQCGADSIGGDPITHLNLSSACHARAA
                     REICLLADRLGHGRVLALGGGGYDRGNLARTWNGVVEALLAA"
     gene            2191..3864
                     /gene="ettA"
                     /locus_tag="VNZ06_06065"
     CDS             2191..3864
                     /gene="ettA"
                     /locus_tag="VNZ06_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HWW20350.1"
                     /translation="MAQYIYTMNRVSKVVPPKRTILRDISLSFFPGAKIGVLGLNGSG
                     KSSLLRIMAGIDTSIDGEARPQPGIKVGFLPQEPQLDPDKDVRSVVMEGLGGTFALVE
                     RFNHISERFAEPMEDEEMNKLLEEQAKLQDAIDAAGGWELGRTLEIAADALRLPPWES
                     KIAQLSGGEKRRIALCRLLLSQPDMLLLDEPTNHLDAESVAWLERYLEEYPSTVVAVT
                     HDRYFLDNVAKWILELDRGHGIPWQGNYSSWLEQKEKRLELEQRESEALRKTLARELE
                     WVRTNPKARQAKSKARLARYEELASREFQERNETNEIYIPPGPRLGDLVVEVKGLRKG
                     FADRLLIDNLSFSLPRGGIVGIIGPNGAGKTTLFRMLTQAEKPDAGDIKIGQSVKLAY
                     VDQSRHTLDDKKTVWSEISGGLDIIKVDNYETPSRSYVGRFNFKGTQQQQLIGELSGG
                     ERNRVHLAKVLRAGGNVLLLDEPTNDLDVETLRALEEALLNFPGCAVVISHDRWFLDR
                     IATHILAFEGNSEVVWFEGNYQDYVEDLKRRRGDDAAQPHRLKYRRLTG"
     gene            3945..4661
                     /locus_tag="VNZ06_06070"
     CDS             3945..4661
                     /locus_tag="VNZ06_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20351.1"
                     /translation="MSVFIDSGDRLHHDQAATLALTCPHCLVVAHITPQAAPRFCELT
                     AVRPKQVGLVYRCDSCNSPIFLKHTVRNYTPARIELSPQFIEIERPKEKFSYTYLPEA
                     VEVMFREALSCYSNGALNAFVTMCRRTMQAVFAQSGESGRLRVFDELSDVRDMAEIDA
                     DSFSAIKRVIFGTDADPPPGLPTLDDDQCGLLLEVMKDLLYQTYVRKGHLQQAMVVRR
                     FFSDEARSIKAAQHAEGQSG"
     gene            complement(4680..>7360)
                     /gene="purL"
                     /locus_tag="VNZ06_06075"
                     /gene_synonym="purI"
     CDS             complement(4680..>7360)
                     /gene="purL"
                     /locus_tag="VNZ06_06075"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HWW20352.1"
                     /translation="RGYHKPIMLAGGLGNVRRSQVEKGEVSVGAALVVLGGPAMLIGL
                     GGGAASSMGSGASEEELDFASVQRGNAEIERRAQEVIDRCCALGADNPIELIHDVGAG
                     GLSNAVPEAVAHSQRGARIELRDVPNAESGMSPMEIWCNEAQERYVLALRPGASTQFA
                     HIAQRERCPFAVIGTLTDDGVLVVHDRAFGNEPVHMPLEALLGKPPRMRRVVKRLPQV
                     RPARDCEQLDVREALYRVLRLPAVADKTFLITIGDRTVGGLISRDQLVGPWQVPVADV
                     AVSLADYHHHAGEAMAIGERTPVALIDAAASARLAVSEAVTNLLAADIASLRDIRLSA
                     NWMAACGEEGEDAALYAAVRAVGMQFCPALGIAIPVGKDSLSMKTVWQERGEQRAVLA
                     PVSLIVSGFAPVRDVRRTLTPVLLRDRGASALLLVDLSLGRNRLGASAVAQVYNAVGG
                     PAPDMDEPARLASFAAAMTELRARGGVLAYHDRSDGGLIVTLIEMAFAGHCGLIVQIQ
                     PQGSSALAQLFAEEPGAVLQIAVSEQGAVMDCFVRHGLRECVQWIGEPSPDLRIRIAC
                     GATLIDESWRELRQAWSETSYRMRRLRDDPQCADEELASALDEHDPGLAQHLSFDPAQ
                     DVAAPMIATGVRPKVAVLREQGVNSQVEMAAVLDRAGFEPHDVHMSDLLSGERSLREF
                     AGLIGCGGFSYGDVLGAGGGWARSILFHESARDEFRRFFERRDTFSLGVCNGCQMFAV
                     LKELIPGAQHWPRFVRNRSEQFEARLSLVEITRSASIVLAGMEGSRLAVAVAHGEGRP
                     QFQTDTDLQQCRARGLIAFRYLGNDGAIARAYPANPNGAVDAIAALTTPDGRVTITMP
                     HPERVYRTAQNSWHPRGQGEDSGWMRMFRNARVWLG"
BASE COUNT         1383 a         2475 c         2297 g         1205 t
ORIGIN      
        1 cggcggccgc ggatcgttcg gcacggcatt cttgttgacg gtgatatggg ccttgccaag
       61 cgccgcgtcc gcctccttac cggtgatcgg acgcccgacc aggctcagca ggaacaggtg
      121 attgtccgtg ccaccggaaa cgatgtggta gccgcgctcg accagcaccg cgcacatggc
      181 ccgcgcattg gccacgacct ggcgctgata ctgcttgaac tccggctgca acgcttccag
      241 cagcgccacc gccttggctg caatcacgtg catcagcgga ccgccctggg taccgggaaa
      301 caccatcgaa ttgatcttct tggtgatggc ctcattggcg cgcgccagga tcattccgcc
      361 gcgcggaccg cgcagcgttt tgtgcgtcgt ggtggtcacg acatcggcgt gcggtaacgg
      421 attgggatac tcgccagccg caaccaggcc ggccacatgc gccatatcga cgaacagcaa
      481 tgcgcccacc acatcggcga tgctgcgcag ccgggcccag tcgatcacgc gcgaataggc
      541 cgaaaaaccc gcgaccagca tcttcgggcg atgctcgagc gtcagccgct cgatctgccc
      601 gtagtccatc agtccggttt cagggtgcac gccgtactgg tgagcgtgga acagcttgcc
      661 ggagaagttg accttggcgc cgtgcgtcag gtgaccgcca tgatccaggc tcatgccaag
      721 aatcacatcg cccggcgtca gcaacgaaag atacaccgcc gcgttggcct gcgagccgga
      781 gtgcggctgc acattcacat agtcggcacc taacagcttg cgggcgcgat cgatcgccag
      841 ttgctcggcg atgtccacga attcacagcc accgtagtag cgcttgccgg gatacccttc
      901 ggcgtatttg ttcgtgagca ccgagccctg ggcctcgagc acgcgcgggc tggcgtagtt
      961 ctccgaagca atcagctcaa tatgatcttc ctgtcgcctg cgctcaccgt cgagcgagcg
     1021 ttgcagcatc gggtcgaatt cgctgatggt catccggctt gaaagcatgc tttgtggccc
     1081 ttcctgtcaa tgcttgcggt cgcgggaagg tgcgggattg tacccgatcg ggtcggatca
     1141 ggcggcgagc agcgcctcga ccaccccgtt ccaggtgcgc gccagattgc cgcggtcgta
     1201 tcccccgccg ccgagcgcca gcacgcgccc gtggccaagc cggtccgcca gcagacagat
     1261 ctcgcgcgct gcgcgcgcgt ggcaggcact cgataggttc agatgcgtaa tcggatcgcc
     1321 gccaatactg tcggcgccgc actgaaggat gaaaaatgcc ggactgaagc gctccacatg
     1381 cgccagcatc tgtggccaga cctgctcgaa ctccacgatc ccggagcctg cgggcagcgg
     1441 cacattgagc ttggtgccct cagccgcgcc gcgtccggtt tccgcatccg agccggtgcc
     1501 cggatagagc gattcaccgc tctcgtgcag gtcggcgaaa atcagatccg gatcgctctc
     1561 aaaaccgtag aacacgccat cgccgtggtg cgcgtcgatg tcgatgtaag cgatccgcgc
     1621 catgccgtaa gtacgccgca agtgctcgac cgcgacaccg caatcgttga acacacagaa
     1681 gccggcggca cgattgcgcg cggcgtggtg caaaccggcg atcggcacga agccgcggcg
     1741 ccgagtccca cgcatgatcc agtccgtggc ggtcagcgtc gatccgacga cgcaggatgc
     1801 ggcttcaaac acgccgcgat aagcgggggt gtcgccgcca tccaggtagc cgatgccgga
     1861 ctgcgagcgc tccatgatga agtgcacgta ctcggcggtg tgaaacagct gcaattcggc
     1921 gctggtggca tcgcgtggct cgagtgtgac cacttggcgt ggcagaccgc gttgctcgaa
     1981 ctcgcgcagg aacgccgcgt gacggtccgg accgaacgga tggccgtcgc cgaagccgta
     2041 gcgcgccagc cgctcaccga tgacgactgc aacctcagag ccgatggcgt tcaagcgctt
     2101 cgcttcgagc cttgggcctg cgacatcgca ggccgccgaa gatatcacac cgcggttccc
     2161 cgcactggcg cgttcatcga taatgtcccc atggcgcaat acatctacac catgaatcga
     2221 gtctccaagg tggtcccgcc caagcgcacc atcctgcggg acatcagtct gtcattcttc
     2281 ccgggcgcaa aaatcggcgt gctcggtctg aacggctcgg gcaaatcgag cctgcttcgg
     2341 atcatggccg gcatcgacac cagcatcgac ggtgaagcgc ggccacaacc gggcatcaag
     2401 gtgggcttcc tgccgcagga gccgcagctc gatcccgata aggacgttcg cagcgtcgtg
     2461 atggaagggc tcggcggcac cttcgcactg gtcgagcgct tcaatcacat cagtgagcgc
     2521 tttgccgaac ccatggaaga cgaggagatg aacaagctcc tcgaagaaca ggccaagctg
     2581 caggatgcga tcgatgcggc cggcggctgg gagctcggac gcacgctcga aatcgccgcc
     2641 gatgcgctgc gcctgccacc gtgggagtcg aaaatcgcgc aactgtcggg cggcgagaag
     2701 cgccggatcg cgctgtgccg gctgctgctg tcgcagccgg acatgttgct gctggatgag
     2761 ccgaccaacc acctggacgc cgagtcggtc gcctggctcg agcgctacct cgaggagtat
     2821 ccgagcaccg tggtggcagt gacgcacgat cgctacttcc tcgacaacgt cgcgaaatgg
     2881 atcctcgaac tcgatcgcgg acacggcatc ccctggcagg gaaactattc gtcctggctc
     2941 gagcagaagg agaagcgtct tgagctggag cagcgcgaat ccgaagcgct gcgcaagaca
     3001 ctggcacgcg aactggagtg ggtgcgcacc aatccgaaag cgcgccaggc caagagcaag
     3061 gcgcgactgg cgcgctatga ggaactggcg tcgcgcgaat tccaggaacg caacgagacc
     3121 aacgaaatct acatcccgcc gggtccgcga ctcggcgacc tggtggtcga agtcaaggga
     3181 ctgcgcaagg gctttgccga tcggctgctg atcgacaatc tgtccttcag cctgccgcgc
     3241 ggcggcatcg tcggcatcat cggtcctaac ggcgctggca agaccacgct gtttcgcatg
     3301 ctcacgcagg ccgaaaaacc cgatgccggc gacatcaaga tcggccagag cgtcaagctt
     3361 gcgtacgtgg atcagtcacg tcacacgctg gatgacaaga agactgtctg gtcggaaatt
     3421 tccggcggac tggacatcat caaggtggac aactacgaaa ccccctcgcg cagctatgtc
     3481 ggccggttca acttcaaggg tacgcaacag cagcaactga tcggtgagct gtcgggcggc
     3541 gagcgcaacc gagtgcatct ggccaaagtg ctgcgcgcgg gcggcaacgt gctgctgctg
     3601 gacgaaccta ccaacgatct ggacgtcgaa acgctgcgag cactggagga ggcgctgttg
     3661 aacttcccgg gatgcgcggt cgtcatctcg cacgatcgct ggttcctgga ccggatcgcg
     3721 acccacatcc tcgcgttcga gggcaattcg gaagtggtgt ggttcgaggg taactaccag
     3781 gactacgtcg aggacctgaa gcgccgtcga ggcgatgacg ccgcccaacc gcaccgtctc
     3841 aaataccgac gtctgaccgg ctaaaaccgg gcgcatccgg tggattatgt cgtgtgtgtg
     3901 acactacggt ggcgctgtgt cgccgttcgg ctacactcaa acgcatgtcg gtgtttatag
     3961 attcagggga tcggctgcac cacgaccagg ccgcaacgct ggcactgacg tgcccgcatt
     4021 gtctggtggt cgcgcacatc accccgcagg cggcgccacg cttctgcgag ctgaccgccg
     4081 tccggccgaa gcaggtcggc ctggtgtatc gctgcgacag ctgcaattcg cccatcttcc
     4141 tgaagcacac ggtgcgcaac tacacacccg cgcgcattga actgtccccg cagttcatcg
     4201 agatcgagcg gccaaaggaa aaattcagct acacctatct gcccgaggcg gtcgaggtga
     4261 tgtttcgcga agcgctgtcg tgctattcaa acggcgcgct caatgcattc gtcaccatgt
     4321 gccggcgcac gatgcaggca gtgtttgctc agagcggcga gtcggggcga ctgcgcgtgt
     4381 tcgatgagct cagcgacgtg cgcgacatgg ccgagattga cgcggacagc ttctcggcca
     4441 tcaaacgcgt gatcttcggc accgatgccg acccgccgcc cggactgcca acgctcgatg
     4501 atgaccagtg cgggctgctg ctggaagtga tgaaggatct gctctatcag acctatgtgc
     4561 gcaagggaca tctgcagcag gccatggtcg tgcgacgctt cttctccgac gaggcgcgca
     4621 gcatcaaagc ggcgcaacac gccgaaggtc agagcggcta gccatcgtgc ggccgcgtcc
     4681 tagcccagcc acacccgcgc gttgcggaac atgcgcatcc agccactgtc ctcgccttgg
     4741 ccgcgcgggt gccaggaatt ctgcgccgtg cgatacaccc gctcaggatg aggcatcgtg
     4801 atcgtgacgc gcccatcggg ggtcgtcagc gccgcgattg catcgaccgc cccattcggg
     4861 ttggccggat aggcgcgcgc gatcgcgccg tcattgccga ggtagcgaaa cgcgatcagg
     4921 ccgcgcgcgc ggcactgctg caggtcagtg tcggtctgaa attgcggccg cccctcgccg
     4981 tgtgcgaccg cgaccgccag gcgcgagccc tccataccgg cgagcacgat cgacgctgat
     5041 cgggtgatct ccaccaggct caggcgcgcc tcgaactgct cgctgcgatt gcgtacgaaa
     5101 cgtggccagt gctgcgctcc gggaatcagc tccttcagca ccgcaaacat ctgacagcca
     5161 ttgcagacgc cgagcgaaaa ggtgtcgcga cgctcgaaga aacgccggaa ctcgtcacgt
     5221 gcgctctcat gaaacagaat cgagcgcgcc cagccgccgc cggccccgag cacatcgccg
     5281 taggaaaatc ccccgcaacc gatcaatcct gcgaactcgc gcagcgagcg ctccccgctc
     5341 agcagatccg acatatgcac atcgtgcggc tcgaagcccg cgcgatcgag caccgcggcc
     5401 atttccacct ggctgttgac gccctgctcg cgcagcaccg caacctttgg tcgcacgccg
     5461 gtcgcgatca tcggtgcggc cacatcctgc gccgggtcaa aactcaagtg ctgcgccaga
     5521 cctggatcgt gctcatcgag cgcgctcgcc aattcctcat cggcgcactg cggatcatcg
     5581 cgcaggcgcc gcatgcgata ggaggtctcg gaccaggcct ggcgcagctc acgccaggac
     5641 tcatcgatga gcgtcgcacc gcaggcaatg cgaatccgca ggtccggtga gggctcgccg
     5701 atccattgca cgcactcgcg cagcccgtgc cggacaaaac aatccatgac cgcgccctgc
     5761 tcgctgactg cgatctgcag caccgcgccc ggctcctcgg caaacagctg cgcgagcgcc
     5821 gagctgccct ggggttggat ctgcacgatg agtccgcagt gaccggcgaa cgccatttca
     5881 atcagcgtga cgatcaggcc accgtccgaa cgatcgtgat aggccagcac gccgccgcgc
     5941 gcgcgcagct cggtcattgc agcggcaaat gaggcgagcc gtgccggctc gtccatgtcc
     6001 ggtgccggac cgccgacggc gttgtacacc tgcgcgaccg cactggcgcc gaggcgattg
     6061 cgaccgaggc tcaggtccac cagcagcagc gcgctcgcgc cgcgatcgcg cagcagtacc
     6121 ggtgtcagcg ttcggcgcac gtcgcgcacc ggggcgaagc cggagacgat cagcgacacc
     6181 ggcgcgagca ccgctcgctg ctcgccccgc tcctgccaca cggtcttcat cgacagggag
     6241 tccttgccca ccgggatggc gataccaagc gccggacaga actgcatgcc gaccgcgcgc
     6301 acggcggcgt acagtgccgc gtcctcgccc tcctcaccgc acgccgccat ccagttggcc
     6361 gacaggcgta tgtcgcgcag cgaggcgatg tccgcggcca gcagattggt gaccgcttcg
     6421 ctgacggcca gtcgggccga ggcggccgca tcgatcagcg ccaccggtgt acgctcgccg
     6481 atcgccatcg cctcaccggc gtgatggtga taatccgcga ggctgaccgc gacatcggcc
     6541 accggcacct gccagggccc caccagctga tcgcggctga tgagtccgcc gaccgtgcga
     6601 tcgccaatcg tgatcagaaa ggtcttgtca gccaccgctg gcagccgcag cacccgatac
     6661 aacgcctcgc gtacatccag ctgctcgcag tcgcgcgccg ggcgaacctg cggcaatcgc
     6721 ttcaccacgc gacgcatacg cggtggtttg ccgagcagcg cctcgagtgg catgtgcacc
     6781 ggctcgttac cgaaggcccg atcgtgcacg accagcacgc cgtcatccgt gagcgtgcca
     6841 atcaccgcaa acggacagcg ctcacgctgt gcgatgtgcg cgaactgcgt gctggctccc
     6901 gggcgcagcg ccagcacgta gcgctcctgg gcttcgttgc accagatctc catgggcgac
     6961 atgccgctct ccgcattggg cacgtcgcgc aattcgatgc gtgcgccgcg ctggctgtgc
     7021 gcgaccgctt ccggcacagc attggacaga ccgccggctc cgacatcgtg aatcagttcg
     7081 atcgggttgt cggcgccgag cgcgcagcag cgatcgatca cctcctgcgc gcggcgctcg
     7141 atctcggcgt tgccacgctg caccgaggcg aaatccagtt cctcttcgga ggcaccactg
     7201 cccatcgatg aggccgcgcc gccgcccagg ccgatcagca tcgccggacc accgagcacc
     7261 acgagcgcgg cgcccaccga gacctcgcct ttttccacct ggctacggcg cacgttgccg
     7321 aggccgccgg ccagcatgat cggcttgtga tagccgcgca
//
LOCUS       DATMRM010000211         4499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_451382, whole genome shotgun sequence.
ACCESSION   DATMRM010000211 DATMRM010000000
VERSION     DATMRM010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451382"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VNZ06_06080"
     CDS             <1..417
                     /locus_tag="VNZ06_06080"
                     /inference="COORDINATES: protein motif:HMM:NF014408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20353.1"
                     /translation="QHIRRAKATSNICTNQGLLMMAGTVYLSLMGAEGMERVAAASMA
                     RTEQLAQLLTAIGGVRRTFSHPVFHEIVLSLDRPVAAVLAALAERDIQGGLDLTEYYP
                     ELGNALLVCATETKSQLDIEAYARAMTEALRAVRAA"
     gene            420..1910
                     /gene="gcvPB"
                     /locus_tag="VNZ06_06085"
     CDS             420..1910
                     /gene="gcvPB"
                     /locus_tag="VNZ06_06085"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HWW20354.1"
                     /translation="MNQTSNRTLHEKLIFEHSRPGRGARGQWPRAESPGAGVPQNLRR
                     TQRALLPEVSELDVVRHFTRLSQLNFSIDTHFYPLGSCTMKYNPKACNAAALLPGFLG
                     RHPLAPVRHGQGFLSCMFELQEMLKAVTGMRGVALSPLAGAHGEFAGVAMIRAYHRAR
                     ADPARNEIIVPEAAHGTNPATATMCGCVAREVAVGSDGDVDLEALRAAVGPKTAGIML
                     TNPSTLGVFERRIEEVARIVHHAGGLLYYDGANLNAILGKVRPGDMGFDVIHMNLHKT
                     FSTPHGGGGPGAGAVGVSARLLPFLPLPIVGRESDGSYRWLDQKDLPQSIGRLTGFGG
                     NAGVLLRAYVYMRMLGREGMHRVGEFATLNANYLSVQLQKAGYEAAFPQRRASHEFIL
                     TLRRLAQQYGVTAMDVAKRLLDLGFHAPTTYFPLLISECLLIEPTESESKETLDAFVR
                     AMAQILREAREDAEHLHRAPYTMPVRRLDDVRAAKELDLVYQRPAG"
     gene            1910..2284
                     /locus_tag="VNZ06_06090"
     CDS             1910..2284
                     /locus_tag="VNZ06_06090"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003443016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HWW20355.1"
                     /translation="MVSEVREKLRGPKRILRAFGSSFKGFVGAYREEAAFRQELGFAC
                     LATPLGIWLGRNGIERALLVGPVLLVLIVELLNSAIEATVDRIGLERHVLAGLAKDIG
                     SAAVLTSFVVVGVVWALVLTGR"
     gene            2294..3226
                     /locus_tag="VNZ06_06095"
     CDS             2294..3226
                     /locus_tag="VNZ06_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HWW20356.1"
                     /translation="MTALICGSMAYDTMMRFEGRFHEHILPDQIHKLNVSFYVPRMRR
                     NFGGCAGNIAYNLKLLGGNGLIMATVGHDFGPYAKWMARHGLADTYLMTLPEEFTAQA
                     FITSDLDNNQITAFHPGAMNYSYLTKVSDTKGIKLGIVGPDSRQAMLEHAQQFAEIGV
                     PFIFDLGQGMPLFNGEDLRSFIHLAAWVAFNDYEASVVSERTGWSLQDIAKRVRALIV
                     TRGAEGSWIFADGQRLEIPAAPISKVAEPTGCGDAYRAGLLFGLENGLDWETTGRIAS
                     LLGAIKIEADGPQMQHFTLDQFKDRYRSSFGRSL"
     gene            3223..>4499
                     /locus_tag="VNZ06_06100"
     CDS             3223..>4499
                     /locus_tag="VNZ06_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWW20357.1"
                     /translation="MRAIDLRALTLSLALLLGSAAAGAAVTSPSPAAVPPGVAIPVNP
                     EASAADWAATLERIASSVVAIQIDQARSFDTERNVSAQATGFVVDAERGLILTNRHVV
                     TPGPVTAEATFLNREEVQLYPVYRDPVHDFGIYHYDPSKLKFIQPKALPLAPQAAQIG
                     REIRVVGNNAGEQLSILAGTLARLDREAPEYGLGRYNDFNTFYIQAASGTSGGSSGSP
                     VIDIHGRVLALNAGGASGAASSFYLPLDRVRRALQLIQQGKAVTRGTLETEFRYRPFD
                     ELRRLGLQSDTEAEVRKTQPDGTGMLVVWDVQPGSVADGVLEPGDILVKVNGALVTRF
                     DPLEASLDDTVGGNVDLQLQRGGKLYDAKLKVGNLDTITPASFLEIGDAVVHTLSYQE
                     ARAFHQPINGVFVAASGYIFDAAGVPRGAIITEL"
BASE COUNT          803 a         1361 c         1479 g          856 t
ORIGIN      
        1 cagcatatcc gccgcgccaa ggcaacttcc aatatctgca ccaaccaggg cctgctgatg
       61 atggccggca cggtctatct ctcattgatg ggtgcggaag gcatggagcg cgtcgccgcc
      121 gcctcgatgg cgcgcaccga acagctcgcc cagttgctca cggccatcgg cggcgtgcgc
      181 cgcacattct cgcacccggt gttccatgaa atcgtgctgt cgctcgaccg gccggttgcg
      241 gcggtgctgg cggcattggc ggagcgggat atccagggcg gactggatct taccgagtac
      301 taccccgaac tgggcaacgc actgctggtg tgtgccaccg agaccaagag ccagctggac
      361 atcgaggcgt atgcgcgggc gatgaccgag gcgctgcgcg ccgttcgagc ggcatgagca
      421 tgaatcagac gagcaaccga acgctgcacg agaagctgat ttttgaacat tcgcgacccg
      481 gccgcggagc acgcggtcag tggccgcgcg cggagagtcc cggcgcgggc gtgccgcaga
      541 atctgcgccg cacacagcga gcgctgctac cggaagtcag tgagctcgac gtggtgcggc
      601 acttcacgcg cctgtcgcaa ctgaatttct cgatcgacac gcacttctat ccgctgggtt
      661 cgtgcacgat gaagtacaac cccaaggcat gcaatgcagc cgcgttgctg ccgggtttcc
      721 tgggccgtca tccgctggcc ccggtcaggc acggtcaggg tttccttagc tgcatgtttg
      781 agctgcagga aatgctcaaa gccgtgaccg gcatgcgcgg tgtggcgctc agtccgctgg
      841 ccggagcgca cggcgagttc gcgggcgtgg caatgattcg cgcctatcac cgcgcgcgcg
      901 cagatccggc gcgcaacgag attatcgtgc cggaggcggc gcacggtacc aatccggcca
      961 ctgcgaccat gtgcggttgc gtggcacggg aagtggcggt cggcagcgac ggcgatgtcg
     1021 atctggaagc cctgcgcgcc gcggtgggac cgaagaccgc cggtatcatg ctcaccaacc
     1081 cgtccacgct gggtgtgttc gagcgccgca tcgaagaagt tgcgcgcatc gtgcaccatg
     1141 ccggtggttt gctttactac gacggcgcca atctcaacgc gattctgggc aaggtgcggc
     1201 caggggatat gggtttcgac gtgattcata tgaacctgca caagaccttc tccacgccgc
     1261 acggcggtgg cggtccgggt gccggcgcag tcggcgtgtc tgcgcggctg ctgccgtttc
     1321 tgccactgcc gatcgtcgga agggaatccg atggcagcta tcgctggctc gatcagaagg
     1381 atctgccgca atcgatcggc cgattgaccg gatttggcgg aaacgccggc gtgctgctgc
     1441 gcgcctacgt ctatatgcgc atgctcggcc gcgagggcat gcatcgcgtc ggtgaattcg
     1501 cgacgctgaa cgccaattac ctgtcggtgc agttacagaa ggcaggctat gaggcggcgt
     1561 tcccacagcg tcgcgccagc cacgaattca ttctgacgct caggcggctg gcccaacagt
     1621 acggcgttac cgcgatggac gtggcgaagc ggctgctcga tctcggattt catgcgccca
     1681 ctacctactt cccgctgttg atcagtgagt gtttgctgat cgagccgact gagagcgaaa
     1741 gcaaggagac cctggacgcg tttgtgcgtg ccatggcgca gatcctgcgg gaagcacgcg
     1801 aggatgcgga acatctgcac cgcgccccgt acaccatgcc ggtgcgccgg ctggacgacg
     1861 tgcgcgcagc gaaagagctg gatctggtct atcaaaggcc agccggctga tggtcagcga
     1921 agtccgcgaa aagctgcggg gcccgaaacg gatcctgcgt gcctttggct cgagcttcaa
     1981 aggcttcgtg ggcgcctatc gcgaagaggc cgcgttccgg caggaactgg ggttcgcatg
     2041 cctggcgaca ccgcttggca tctggctcgg gcgcaacggc atcgagcgcg cgctgctggt
     2101 cggtccggtg ttgctggtgc tgatcgtgga gctgctcaac agcgcgatcg aagcgacggt
     2161 cgatcgcatc ggtcttgagc ggcacgtgct ggccgggctg gcgaaggaca tcggctccgc
     2221 tgcggtgctg acctcgtttg tggtggtcgg cgtggtgtgg gcattggtct tgacgggacg
     2281 ttaaaggata gcgatgacgg cactgatctg cggttcgatg gcatacgaca ccatgatgag
     2341 gttcgagggg cgctttcacg agcacatcct tcccgatcaa atccacaagc tcaatgtgtc
     2401 gttctacgtg ccacgtatgc ggcgcaattt tggcggctgc gccggcaata tcgcctataa
     2461 cctgaaactg ctcggcggca acggattgat catggcgacg gttgggcacg actttggtcc
     2521 gtacgccaaa tggatggcgc ggcacggact tgccgacacg tacctgatga cgctgccgga
     2581 ggagtttacc gcgcaggctt tcattacgag tgatctggat aacaaccaga tcacagcatt
     2641 tcatcccggt gcgatgaact attcatacct gactaaggtc agtgatacga aaggaatcaa
     2701 gctcggtatc gtcggaccgg acagccgtca ggcgatgctc gaacatgcgc agcagttcgc
     2761 tgaaatcggg gtaccgttca ttttcgacct gggtcagggc atgcccttgt tcaatggtga
     2821 ggatttgcgc agtttcatcc atcttgccgc ctgggtggca ttcaacgact acgaagccag
     2881 cgtggtatcc gagcgcaccg gctggtcgct gcaagacatt gccaagcgtg tcagggcact
     2941 gatcgtgacg cgcggcgccg agggttcctg gatttttgcc gacggccaac gactggaaat
     3001 ccccgccgcg cccatttcaa aggtggcgga gcctaccggg tgcggggatg cctatcgagc
     3061 cggcctgctg ttcggtctgg agaacggact cgactgggag acgaccgggc gtattgcctc
     3121 actgctcggc gccatcaaga tcgaagctga cggcccgcaa atgcaacatt tcacgctcga
     3181 ccagttcaag gaccgatatc gctcgtcctt cgggcgttct ttgtgagagc aatcgatctg
     3241 cgcgccctga ccctgtcgct cgcgctgctg ttgggtagcg cggcggcggg cgcggcggtc
     3301 acctcgccat cgccggcggc ggtgcctcca ggggttgcca ttccggtcaa tccggaagca
     3361 tccgccgcgg actgggccgc aacgctggag cgcattgcca gcagtgtggt ggcgatacag
     3421 attgaccagg cgcgctcgtt cgacaccgag cgcaacgtgt ccgcgcaggc caccggtttc
     3481 gtggtcgatg cggagcgcgg gctcattctc accaaccgcc atgtggtgac tcccgggccg
     3541 gtcaccgccg aggctacctt tctcaatcgc gaagaggtgc agctctatcc ggtctatcgc
     3601 gatccggtgc atgacttcgg catttatcac tacgatccca gcaagctgaa gttcatccag
     3661 cccaaggcgc tgccactggc gccccaggcc gcgcagatcg gccgcgagat tcgggtggtc
     3721 ggcaacaatg ccggcgagca gctttccatt ctggcgggca cgctcgctcg actggatcgc
     3781 gaggcgcctg aatacggact cggacgctac aacgacttca atactttcta cattcaggcg
     3841 gcgtccggta cctccggcgg ctcgtcgggc tcgcctgtga tcgacattca cggtcgggtg
     3901 ctggcgctga acgcgggcgg agcctcgggc gcggcctcca gcttctattt gccgctcgat
     3961 cgcgtgcggc gagcacttca gctgatccag cagggcaagg cggtcacgcg cggcacgctg
     4021 gagaccgagt tccgctatcg gcctttcgat gagctgcggc gcctcgggct gcagagcgat
     4081 accgaggccg aggtgcgcaa gacacaaccc gatggcaccg gcatgctggt ggtgtgggac
     4141 gtgcagccgg gttcagtggc ggacggtgtg ctcgagcccg gcgatattct ggtgaaggtc
     4201 aatggagccc tggtgacgcg cttcgatccg ctcgaggcca gtctggacga caccgtcggc
     4261 ggtaacgtgg atctgcagct gcaacgtggt ggcaagctgt acgacgccaa gctgaaggtc
     4321 ggcaatctgg atacgatcac gccggcatcc tttctcgaga tcggcgacgc cgtggtgcat
     4381 acgctgtcct accaggaagc gcgcgcattt caccaaccga tcaacggtgt gttcgtcgcg
     4441 gcgtccggct acatcttcga tgcggccggc gtgccgcgcg gtgcgatcat caccgaact
//
LOCUS       DATMRM010000212         4744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3517821, whole genome shotgun sequence.
ACCESSION   DATMRM010000212 DATMRM010000000
VERSION     DATMRM010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3517821"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VNZ06_06105"
     CDS             <1..228
                     /locus_tag="VNZ06_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20358.1"
                     /translation="VGPHDTPPTPNGVGPHDTPPTPNGVGPHDTPPTPNGVGPHTKKT
                     KKKTKKKTVKKKAVKKTKKVAKKKKKAAKKK"
     gene            complement(240..2555)
                     /locus_tag="VNZ06_06110"
     CDS             complement(240..2555)
                     /locus_tag="VNZ06_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWW20359.1"
                     /translation="MTGRLKAPIQIGDWIADPQTDTISRRGETQKLEPRTMRLLLLLA
                     ERPGEVLSVDVMLNEVWHGVVVGSASVYQAVSQLRRTLGDSDAAPTYLATVPRKGYRL
                     IAAVSAVTPRACTQLTPVSESNAVTVLPATTTEAANQPQVASRRRVNLLWILIAITLV
                     LLAPVIFIARHFLTSPDDITDSIVVLPFIDMTEEHRDQIFCDGLTEELSNWLAQIPTL
                     RVVARTSAFAFRGQHDAREIGKKLNTTHVLEGSIRRSGDNMRVTAQLIDARTGYHVWS
                     SNYDQHIDDTIKTQELIARAVAESLQIRLTPSTTQKFAQRQSANPQAYNLYLLASHYR
                     LERTRESTLQAIELYRQALANDPNFALAYVGLAYAYLNQHWVDTRTVREVAAAAEPLL
                     DTALRLDPQLSELYTVRGALRSEELHLDEAQSDLQHAVAMNPNNSWAFAELGRLYTME
                     GRPRDALEAYQHTLVLDPLDFLHHARQCIALTDLNRYQEAATACARARALQDQGNFGT
                     IATGWLARTRGDIPEALKWNAEALNSEPKDIDLYKQRADLLMTIGLSALARDTYQRAL
                     AATHNDEEVHLGMAAVVYKDGGAAPLRSHLATSGLDGSAQARILLKTAYFHLLAGDDA
                     AAQQAIARARSAPDFNEAEVNDPWFARWGDSDLLTVAQSELRNGDQEAANRHLHEILT
                     LLDREMAAGMDRFGPYALRAQVLALLGDSGGAMQALTRAADLGWRREWWAQHEPFLSA
                     LRGRNDFRALMARVAASNRALIANAQAQLAH"
     gene            2731..3540
                     /locus_tag="VNZ06_06115"
     CDS             2731..3540
                     /locus_tag="VNZ06_06115"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HWW20360.1"
                     /translation="MQMPTEASQPIAIYTLGRFEVALNGTPLKFVFKVPRKPLALLKA
                     LLAGGRNGVSQTALCDTIWPDLEPWSAARALHITAFRLRALLGSKSALIVQEGRVALD
                     PEQCWVDAWQFEHSLAQAKDPTEQLWALRFYRGNFLTDSDHPLALETRDRLSCKFIRS
                     VSQLGASYERIGDGQSAIDLYLMALDADGRCEELHQSLMRCLAREGQPSAVAAAYHRC
                     RASLERHFGTKPSEVTEQIYREACPDTTTRRAHEPALRVVNGHRAGESTPA"
     gene            3720..>4744
                     /locus_tag="VNZ06_06120"
     CDS             3720..>4744
                     /locus_tag="VNZ06_06120"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HWW20361.1"
                     /translation="MRRRLDSLERELRELKLSARRQPGPASASGDTLPATPATPLAPA
                     ATQASASRVATDSAPIRAAPELATRSEWVAPPPEPPAPAMQPAPPPVAQTVIPRLWQL
                     ISGGNPIVRFGVIILFFGVAFLLRYAAEHSHLTIEDRLIGVALGAIALLGAGWFWRNR
                     HRTYAMALMGGGVGVLYLTVFIAYRNYALVSAPAAFALLAVVGGLSAALAILLDSMAF
                     AVLGTVGGYLAPVLASTPRDDHVALFSYYALLTLVVAGIAWYRSWRPLNLVAFVFTYS
                     IGTAWGVLRYRPEDFSTSEPFLILFLLMFVFLAVSFAWSRASQHSHYVDGALVFGAPI
                     VTMGLQAG"
BASE COUNT          853 a         1460 c         1482 g          949 t
ORIGIN      
        1 gtcggaccac acgacacgcc gccgacaccc aatggcgtgg gaccgcacga cacgccgccg
       61 acacccaatg gcgtcggacc acacgacacg ccgccgacac ccaatggcgt cggaccgcac
      121 acgaagaaga ccaagaagaa gaccaagaag aagactgtga agaagaaagc ggtcaagaag
      181 acgaagaagg tagcgaaaaa gaagaagaag gccgctaaga aaaaatagcg accgcgtgat
      241 cagtgtgcca attgcgcctg cgcattggcg atcagcgcgc ggttgctggc tgcaacgcgg
      301 gccatgagcg cacgaaaatc gttccgcccg cgcagcgcgg acaggaacgg ttcgtgctga
      361 gcccaccatt cgcgccgcca tccgagatcg gcggcgcgag tgagtgcctg cattgctcca
      421 ccgctatctc ccagcagcgc cagtacctgc gcccgcagcg cgtagggacc aaagcgatcc
      481 atcccggccg ccatttcccg atcgagcagc gttaggattt cgtgcaggtg acgattcgcg
      541 gcctcctgat ccccgttgcg cagttcagat tgagcgacgg tcagcagatc cgagtctccc
      601 cagcgcgcga accagggatc gttcacttcc gcttcattga aatcaggggc ggacctggcg
      661 cgcgcaatgg cctgttgcgc ggcggcatcg tccccggcca gcaaatggaa gtacgcagtt
      721 ttgagcagga tgcgcgcctg cgccgaccca tcgagtccgc tggttgccag atgtgagcgc
      781 agcggtgctg cgccgccgtc tttgtagacg accgcagcca tgccgagatg aacctcttcg
      841 tcgttgtgcg tggcggcaag tgcccgttgg taggtgtccc gagcgagcgc cgacagtccg
      901 atcgtcatca gcaggtccgc gcgctgcttg taaaggtcga tgtcctttgg ctcactgttg
      961 agcgcttcgg cgttccactt cagcgcctcc gggatgtctc ccctggtacg tgccagccag
     1021 cctgtggcga tggtcccgaa gtttccctga tcctgcaggg cgcgcgcgcg ggcgcatgcg
     1081 gtcgcggcct cctggtaccg attgaggtcc gtgagcgcga tgcattgacg cgcgtggtgc
     1141 aggaaatcca gcggatcgag caccagcgtg tgctgatagg cttccagcgc atcacgcggg
     1201 cgcccttcca tcgtgtagag ccgaccgagc tcggcgaacg cccagctgtt gttcgggttc
     1261 atcgccacgg catgctgcag atcgctttgc gcttcgtcca gatgcagctc ctcagagcgc
     1321 aacgcgccgc gcaccgtgta cagctcggaa agttgtggat caagccgcag cgcggtgtcc
     1381 agcaagggtt ccgcggcagc ggccacttcg cgcacggtac gcgtatcgac ccagtgctgg
     1441 ttcagatagg cgtacgcgag gccgacatag gcgagggcga agttcggatc gttcgccagt
     1501 gcctgccgat acagctcgat ggcctgcagc gtcgactcgc gggtgcgttc cagccgatag
     1561 tgactggcaa gcaggtatag gttgtaggcc tgcggattgg cactctggcg ctgggcgaat
     1621 ttttgcgtcg tgctgggtgt cagccggatc tgcagactct ccgcgaccgc gcgggcgatc
     1681 agctcctgcg tcttgatggt gtcatcgata tgttggtcat agttggaaga ccagacgtga
     1741 tagccggtac gggcatcgat caactgggca gtcactcgca tgttatcgcc agatcggcgg
     1801 atggaacctt ccagcacatg cgtcgtgttg agcttcttgc cgatctcgcg cgcatcatgc
     1861 tggccacgga acgcaaaggc ggaggttcgc gccaccaccc gcagagtggg aatctgggcc
     1921 agccagttcg acaactcttc agtgagaccg tcgcagaaga tctggtcccg gtgctcttcc
     1981 gtcatatcaa tgaacggcag taccacgatg gagtccgtga tgtcatccgg cgacgtcagg
     2041 aagtggcgcg cgatgaatat gacgggggcc aacagaacga gcgtgattgc gatcaggatc
     2101 cacagcaggt tgacgcgacg cctcgacgcg acctgcggct gattggcagc ctcggtcgtt
     2161 gtagccggaa ggactgttac cgcgttggat tcggatactg gcgttagctg agtacacgcc
     2221 cgcggggtga cagcactgac tgccgcaatc agccgatatc cctttcgcgg aactgtcgcc
     2281 agataggtag gcgctgcatc ggagtccccc agggtgcgcc gtagttgcga gaccgcctgg
     2341 tacaccgatg ccgatccgac caccacgccg tgccagacct cgttgagcat cacatcgacg
     2401 ctcaggacct ccccgggtct ttctgccaat agcaatagta accgcatcgt gcgcggctca
     2461 agtttttgag tttcgccgcg acgcgagatg gtgtcggttt gggggtcagc aatccaatcc
     2521 ccgatttgaa tgggcgcctt caagcgtcct gtcatcgacc ctgcctcgac tctttcattg
     2581 ggaagtatag ctgcgatccg gcaacagata agtacctgaa tttggaggca ttgagaaatg
     2641 cttcagcatt tcttcagact ttcttgagga ctacggattc tttaatccgt acatcactcg
     2701 atatcgtcaa ttgggaggga tggacgcgtc atgcaaatgc cgaccgaggc ttcacagcca
     2761 atcgcaattt acactttggg gcgcttcgaa gtggcgttga acggcacgcc actgaagttc
     2821 gttttcaagg ttccccgcaa accgttagcc ttgttaaagg ctctgctggc agggggtcgg
     2881 aacggagtca gccaaacggc actctgcgac accatttggc cggatctgga accgtggtcc
     2941 gcggcccgtg cgcttcatat caccgcattc cggctccggg cgctgctcgg cagcaagtcc
     3001 gcgctgatag ttcaggaggg aagggttgcg ctggacccgg agcaatgctg ggttgatgcc
     3061 tggcagttcg aacattcgct ggcccaggcc aaggatccca ccgagcagct ttgggccctg
     3121 cggttctatc ggggcaactt cctgaccgat tcggatcatc cgctggcatt ggagacgcgc
     3181 gatcgactga gctgcaagtt catccggtcg gtgtcgcaac tcggcgcaag ctacgaacgc
     3241 atcggcgacg ggcagtccgc gatcgacctg tacctgatgg cgctggatgc ggacggtcgc
     3301 tgcgaggaac tgcatcaatc gctgatgcgc tgcctggcgc gcgaaggtca accttcggca
     3361 gtggctgccg cctaccatcg ctgccgggcg tcgctggagc gtcattttgg taccaagcct
     3421 tccgaagtga ccgagcagat ctatcgcgag gcctgtccgg acacgaccac ccgtcgcgcg
     3481 catgagccgg cgttgcgtgt cgtcaatgga catcgcgcgg gagagtcaac ccctgcctga
     3541 gcggtagcaa tatacggggg tgccacctat cctaggtggc accccgactg tgcagctccg
     3601 acactgatag accagggatc tcatccgcct gagcctcttc ggttgcaggc gtcaggctaa
     3661 tgctcggata cgccatcctg gccgtgatcg tcattgtttg gctagctgat cgggccgact
     3721 tgcgcaggcg tttggatagc ctggagcgcg agctgcgtga actcaaactc agcgcccgtc
     3781 gccaacccgg cccggcatcg gccagcggcg acacattgcc cgctacaccc gctacaccgc
     3841 ttgcgcctgc agccacgcaa gcgagcgcat cccgggtagc gaccgactcg gcgccgattc
     3901 gcgctgcgcc cgaactcgcc acgcgctcgg agtgggttgc gcccccgccc gaaccgcccg
     3961 cccccgcgat gcaacctgcc ccgccaccgg ttgcgcagac cgtcatcccg cgcctgtggc
     4021 aactcatcag tggcggtaat ccgatcgttc gctttggcgt catcattctc tttttcggcg
     4081 tcgcctttct gctgcgctac gccgccgagc acagtcatct caccatcgaa gatcgcctga
     4141 tcggtgtcgc gctcggagcg atcgccctgt tgggagcggg ctggttctgg cgcaaccgcc
     4201 atcgaaccta cgcgatggca ttgatgggcg gcggtgtggg cgtgctgtat ctgacggtct
     4261 tcatcgccta tcgcaactac gcgctggttt cggcgccggc ggcgtttgcg ctgctggccg
     4321 ttgtcggcgg cttgtccgca gcgctggcga tcctgctcga ctcgatggcg ttcgccgtgc
     4381 tgggtacggt cggtggctac ctggcgccgg tgcttgcctc gacgccgcgc gacgaccatg
     4441 tggcgctgtt ctcctactat gcgctgctca cgctggtggt cgccggcatc gcgtggtacc
     4501 gatcgtggcg cccactgaat ctggtggcgt ttgtattcac gtacagcatc ggcacggctt
     4561 ggggtgtgct gcgttatcgg cccgaggatt tttctaccag cgaacctttc ttgatcctct
     4621 tcctgctgat gttcgtattt ctggccgtgt cgtttgcgtg gtcgcgtgcc agtcagcaca
     4681 gccactatgt tgacggtgca ctggtgtttg gagcgccgat cgtcacgatg ggactgcagg
     4741 cggg
//
LOCUS       DATMRM010000213        16472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1974635, whole genome shotgun sequence.
ACCESSION   DATMRM010000213 DATMRM010000000
VERSION     DATMRM010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16472
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1974635"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VNZ06_06125"
     CDS             <1..193
                     /locus_tag="VNZ06_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HWW20362.1"
                     /translation="ADEVHPDDHVFESSGVKVFVDPKSLPSVDGTRIDFVRQGLNESF
                     RFENPNVRAECGCGESFTV"
     gene            263..691
                     /gene="ndk"
                     /locus_tag="VNZ06_06130"
     CDS             263..691
                     /gene="ndk"
                     /locus_tag="VNZ06_06130"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HWW20363.1"
                     /translation="MALERTLSIVKPDGVSRNLIGNVYQRLEKAGLRIVAARMLRLTQ
                     PQAELFYEVHRERPFYKDLVRYMTSGPVIAQVLEGESAISKNREIMGATDPKKAAAGT
                     IRADLAQSIEHNVVHGSDAPETAAREIAFFFSSTEICPRL"
     gene            676..1791
                     /gene="rlmN"
                     /locus_tag="VNZ06_06135"
     CDS             676..1791
                     /gene="rlmN"
                     /locus_tag="VNZ06_06135"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWW20364.1"
                     /translation="MSTPVNLLGLPRPDLESFCAELGSKPFRARQLMNWLYKRGCDRF
                     DQMTDLALDFRACLVERAEVRLPEIITVQRASDGTCKWLLRADASQAFEMVYIPEVDR
                     GTLCISSQVGCALDCAFCSTAQQGFNRNLSTAEIVGQVWLANRELGFQPGGDRIITNV
                     VLMGMGEPLANFRNVIAACRIFLDDLGMDLSRRRVTLSTSGLVPQIYRLAQEINVALA
                     VSLHAPDDELRDRLVPINRLHPIAELLEACWHYIDEQNGRSVTFEYVMLEGVNDSPLQ
                     ARRLSKLLAGHPAKVNLIPFNPYPGAQFHRSGDEAIVRFRDELNRAGVIATIRRTRGD
                     DIDAACGQLVGRVIDRTVVRLGAKAAARLSRAAGMNA"
     gene            1788..2600
                     /gene="pilW"
                     /locus_tag="VNZ06_06140"
     CDS             1788..2600
                     /gene="pilW"
                     /locus_tag="VNZ06_06140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HWW20365.1"
                     /translation="MKRMLLSVVSGLALASCHSTPTVNPSVGSNANPGRPTTSGGPTQ
                     TQRAAAARANMTLGLGYLQQGNLPIAKEKLDKARSEDPDLPDIHGAMALLDERLGKDK
                     EADKEFRAALKIDPHDPAMLNNYAVFLCSHGKPDEGVRNFEQAASNPLYSTPWAAYTN
                     AGVCLRAAHRDAEARPRFDRALRSNPAYSEAVFQSADLDLQQQKYPEARFLIDLFLLR
                     NPATPDLLLLAWRVAQAQNDPTAQQKYAARLAQEFPNSEQSRALTPTRPNPG"
     gene            2604..3584
                     /locus_tag="VNZ06_06145"
     CDS             2604..3584
                     /locus_tag="VNZ06_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HWW20366.1"
                     /translation="MADGEGTSLTSSPGARLAVARRQAGMSTSEAAERLHLNPQTLEA
                     LEAGRMEALGAAVYARGHLKRYAELVGVAESEILAAYDAWSGRPAALPDLREVITAPA
                     VRSGTRRFELKAGHALTGAIVLVLVMVVWWAMRKPSRVSERAVPAAAVQSAAPAPAAP
                     TLTPSSIPAPTPAAPPPAPAPPVAATVRPVAPSSAQSRPPPVAVSAPLVNAPAVSAPA
                     TVSVSGPPARLALNFSENSWIEIYGADGAQLFHGMAQAGSQHRVTGIAPLRVFLGNPP
                     AVTLEMNGHPVVMTGNPKPRRFSLDDAGHIVEVRASDTSAKGPTAQPRSD"
     gene            3601..4869
                     /gene="hisS"
                     /locus_tag="VNZ06_06150"
     CDS             3601..4869
                     /gene="hisS"
                     /locus_tag="VNZ06_06150"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HWW20367.1"
                     /translation="MSKQIQPVRGMNDVLPAQIALWQHVERVARGIFESYGYQEIRVP
                     VVEHTELFKRSIGEFTDIVEKEMYTFQDLGGDSLTLRPEATAGIVRSAISNGLLRGAR
                     HKLWCIGPMFRHERPQKGRYRQFYQLDLEAIGFPGPDVDIELIALISRLWRELKLTQV
                     RLLINSLGTAAARAVYRERLQEYLRLHESELDEDSRRRLEGNPLRVLDSKNPAMRSVI
                     ERAPVLTDFLDSESAEHFARLRAGLDALGVAYEVNPRLVRGLDYYSRTVFEWVTDALG
                     AQDAVCSGGRYDGLTAQLGGEATAAIGFAMGIERVVELVSLSGSTPVVATPEIYIVAS
                     GSRAELEALRLAERLRDALPGRGVLVNLGGGNFKTQFRRADKSGARLALILGDAELDR
                     GVAAVKTLRSEAGQMDCPLGELPARIASWP"
     gene            4889..5536
                     /locus_tag="VNZ06_06155"
     CDS             4889..5536
                     /locus_tag="VNZ06_06155"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWW20368.1"
                     /translation="MDEYLSEREQWERVLQWLREQGPWVLVSIAVVMTGYYGWNYWHS
                     RVEQRYVTAATRYEAVLQSFRNRDGKGEPNLDAGLKLTEQFIKDYPSTGYADQANLFA
                     AGALVENRSFDQAASRLAQVMNSTKDPELGLIARLRLARVQLAQNKPDEALKTLSAVE
                     PGAFAARYSQVRGDALFAKGDREGALREYRAARSAAGDTIDTGLLDLKIDELAHS"
     gene            5533..6771
                     /gene="bamB"
                     /locus_tag="VNZ06_06160"
     CDS             5533..6771
                     /gene="bamB"
                     /locus_tag="VNZ06_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HWW20369.1"
                     /translation="MSTERLSLRGWSAVLLAVLLGVVLLPGCSHKKDTKKTNDKPMAL
                     VPVTNHFEVRRVWSNRISGESPKLRLGLGVAASGERVFVASHKGVVDAFALDSGKRLW
                     RVRLKAPLSGGPSAGHDRVAVGTSKGEVILLSETDGSVLWRVRVNSEILAGPAIGADL
                     VVVRTVDGKMHGLAVKDGVESWVVDQQVPKLSLRGTSAPTLSGDLAITGFDNGRVVAV
                     NRRNGSTVWDAAVGQSHGSTELARLIDVDSPVVVEGEDLFTVAFQGRVVHISRDTGQV
                     LWTHDVSSYRGLAIDADSVYVSTADGDIVRLDRKNGTEQWRQKAMERRQLTAPVLYEG
                     YPVVADGGGVLHWLNPANGDVVARAEVDKSSSRNALKSKGISLKRRISSEPIAAGGLL
                     LVFTDSGLLSAFRSPPPGGK"
     gene            6781..8163
                     /gene="der"
                     /locus_tag="VNZ06_06165"
     CDS             6781..8163
                     /gene="der"
                     /locus_tag="VNZ06_06165"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HWW20370.1"
                     /translation="MLPIVAIVGRPNVGKSTLFNALTRSQAAIVADVPGVTRDRQYGY
                     SRIGAVPCVLIDTGGLIENPTGLEAQMRVQTEKAVAEADRLILVADARAGMTSQDQFI
                     ARELRRTGKPVVLALNKAEGLDEDMVAADFHTLGLGRPLAIAASHGQGCRELIDEVLS
                     GMQADAAPAEDTSAIRVAIIGRPNVGKSTLVNRLLGEERVIASEQPGTTRDSILVPFQ
                     RDGRDFLLVDTAGVRRRGKVADAVERASVARTLQAIAESHVVILVLDAHDAIGEQDAS
                     VLGQALDRGRVILIAINKWDGIATEQRDEIRRQLSLKLDFVPFAPLHFISARHGTGVG
                     ELARDVIRGYDAAMRAMATPELTRTLERALQAHQPPLVRGRRIKLRYAHQGGRNPPRI
                     IVHGNQTASVPEAYRRYLANVFRETFDLYATPVAVDFRTDANPYDRKRAGKVRARRAA
                     GTMPGRQRRG"
     gene            complement(8111..8953)
                     /locus_tag="VNZ06_06170"
     CDS             complement(8111..8953)
                     /locus_tag="VNZ06_06170"
                     /inference="COORDINATES: protein motif:HMM:NF037811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWW20371.1"
                     /translation="MKSVRACVLAAALTLSAVPGVFALDLPQAEAVPGGVVLLPLQQP
                     ADATPVVTFESNRVMVLRQADHWLAVIGIPLSAPIGNTTVSIAASAGAPASSQSFQVG
                     DKNYTTQRLTVSPAKVDLSGRDQARAARERSHLQTALSSFSEQPPSTLRLLPPVPGAR
                     SSSYGSRRVFNGEARNPHSGMDIPAPQGLAVHAAADGRVIDTGNYFFNGNTVLLDHGE
                     GLITMYCHLSQISVHVGEVVHAGGIIGKVGATGRVTGPHLHFGVALNHAFVDPALFLP
                     PAEP"
     gene            complement(8950..10278)
                     /gene="xseA"
                     /locus_tag="VNZ06_06175"
     CDS             complement(8950..10278)
                     /gene="xseA"
                     /locus_tag="VNZ06_06175"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HWW20372.1"
                     /translation="MQHEFFDPPSAAKAGTPEPPARQRTIYSVSDLNREVRLLIESGI
                     GNVWVQGELSNLSRPASGHWYFSLKDGEAQLRCCMYRQRNILARFTPREGQLLLARGR
                     VSLYEPRGEFQLQIEHLEDAGLGALQRAFDDLKRKLEAEGLFAAERKRALPTAPRLIG
                     VITSPSGAAIRDILNILGRRFPSARVLVHPVPVQGDAAPRAIVRALDLASARAECDVL
                     ILARGGGSLEDLWAFNEESVARAIYRCGIPVVAGIGHEIDFTIADFVADVRAPTPSGA
                     AELVAPDTRHWLHRLAQLHRRFLAGMRRTIGVDGERLQALSQRLSRSARVRLAQQVAH
                     LRSLGHRLQQAHPGIRLAQYDQRLRQLDQRLQRAVRVCVAHQAARLDSAARALQAVSP
                     LATLGRGFAVITRSADGALVTSAEQLSVGEDLDAQLANGSVRARVIERGP"
     gene            10381..11847
                     /gene="guaB"
                     /locus_tag="VNZ06_06180"
     CDS             10381..11847
                     /gene="guaB"
                     /locus_tag="VNZ06_06180"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWW20373.1"
                     /translation="MRILEEALTFDDVLLVPARSDVLPREVDLSTQLTRRIRLGLPVL
                     SAAMDTVTEGRLAITMAQEGGIGIIHKNMSIESQAREVAQVKKFESGVITDPITVPPE
                     TTIRQVIELTRARGISGVPVVSGNKVLGIVTHRDLRFERQLDAPVSAVMTPKSRLITV
                     RENAPKEEVQRLLHKHRIEKVLVVNGDDELRGMITVKDFQKATEFPRASKDVSGRLRV
                     GAAVGTSPDTLDRVSALREAGVDVIVVDTSHGHSRGVLDMIAKLKSRWSDLELIAGNI
                     VTREAALDLVAAGTDAVKVGIGPGSICTTRVVAGVGVPQISAVANVAAALEGKPISII
                     ADGGIRYSGDLAKAIAAGAHAVMIGGMFAGTEESPGEVELYQGASYKAYRGMGSLGAM
                     AERYGSADRYFQDTASELEKLVPEGIEGRVTYKGSLVTILYQLAGGLRAAMGYTGSHS
                     IEEMRTRPVFVRVTTAGVRESHVHDVTITKEAPNYRIS"
     gene            11882..13456
                     /gene="guaA"
                     /locus_tag="VNZ06_06185"
     CDS             11882..13456
                     /gene="guaA"
                     /locus_tag="VNZ06_06185"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002552500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWW20374.1"
                     /translation="MATDIHAHRILILDFGAQYTQLIARRVRELGVYCEIHPWDIDDA
                     EVKRFGARGIILSGGPESVTDATPPTAAQCVFELNVPVLGICYGMQTMAQQLGGRVAA
                     GSVREFGYAEVQVSAACRLLDAISDRLSDGHAILDVWMSHGDRVEALPAGFGVVASTH
                     SAPLAAMADERRHFYGVQFHPEVTHTRQGTAILERFVRDICACEARWTASSIIDDLIA
                     RIRAQVGTDQVLLGLSGGVDSSVVAALLHRAIGPQLTCVFVDHGLLRLGEGDQVMRTF
                     ADSLGVHVIRINAELRFLSALRGQTDPEAKRKIIGRLFIEVFEQEARKLRDTRFLAQG
                     TIYPDVIESAGARTGKAHVIKSHHNVGGLPADLKLKLVEPLRELFKDEVRRIGVELGL
                     PHDLVHRHPFPGPGLAVRILGEVKHEYAELLRRADHIFIEELRRHSLYERTSQAFAVF
                     LPVRSVGVMGDGRRYDYVIALRAVETIDFMTAHWSHLPYEFLDLVSRRIVNEVPGISR
                     VVYDISGKPPATIEWE"
     gene            13510..14040
                     /locus_tag="VNZ06_06190"
     CDS             13510..14040
                     /locus_tag="VNZ06_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWW20375.1"
                     /translation="MAKLVYGLNQSLDGYVDHLEFRPSLALFRHFIEHVRDLTGIVYG
                     RRMYEVMRYWDEDLADWVAEEHDFAAAWRSQPKWVVSRSLKSVGPNATLVENDIEAVI
                     RGLKARPVGQIEVAGPDLAGSLTNLGLIDEYRLYLHPVVLGRGKPFFAGPRPPLRLVT
                     SDRIAEDVIRLTYVPA"
     gene            complement(14240..14641)
                     /locus_tag="VNZ06_06195"
     CDS             complement(14240..14641)
                     /locus_tag="VNZ06_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWW20376.1"
                     /translation="MYLLDTNIVSELRKRRPNPKVVAWVQAVKDPDLHLSAVTIGEIQ
                     AGIEVTRSQDPTKAAELEAWADQLADSFNVLVMDAACFRRWAKLMHRQSSSVYEDAMI
                     AATALVHDLTVVTRNMRDFSRFDVQSLNPFE"
     gene            complement(16098..>16472)
                     /locus_tag="VNZ06_06200"
     CDS             complement(16098..>16472)
                     /locus_tag="VNZ06_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20377.1"
                     /translation="PVFCFFLAKVVRHFPRLSALIFTVAVPLMITYCVEVLIVLIRGV
                     LGARALIGPLYFLLHVALTFSAAPALACVLLLGRRSFASWWLLAAAICWVVGAGSIFY
                     QYDVAETLYGIDGLGGPYHWPW"
BASE COUNT         3064 a         5110 c         5368 g         2930 t
ORIGIN      
        1 cgccgatgaa gttcatccgg acgaccacgt ttttgaaagc tcgggcgtca aggtgttcgt
       61 ggatccgaag agcctgccca gcgtcgacgg cacgcgcatc gatttcgtgc gccagggcct
      121 gaacgaaagc ttccgcttcg aaaatcccaa cgtgcgcgca gagtgcggct gcggtgagag
      181 cttcacggtc tgataatccc taatcggcga ggccttggaa caccggtata ctgccgccct
      241 tttttgagca cggaggttcc gcatggcgct cgagcgcacc ctgtctattg tcaaacccga
      301 tggcgtcagc cgtaacctga tcggcaacgt ctaccagcga ctcgagaagg cgggactgcg
      361 gatcgttgcc gcgcggatgc tgcgtctgac gcagccgcag gcggaactgt tctacgaggt
      421 gcacagggag cgtccgttct acaaggatct ggtgcgctac atgacctccg gcccggtcat
      481 cgctcaggtg ctggagggcg agagcgcgat cagcaagaac cgcgagatca tgggtgcgac
      541 cgatccgaag aaggccgctg cgggtaccat tcgtgcggac ctggctcaga gcatcgagca
      601 caatgtggtg cacggctcgg atgcgccgga gaccgccgca cgcgagatcg cgttcttctt
      661 cagttcgacg gagatatgtc cacgcctgta aatctgctcg gcctgccgcg gcctgacctg
      721 gaaagcttct gcgccgagct gggatccaag ccctttcgcg cgcgccagtt gatgaactgg
      781 ctctacaagc gcggctgcga ccgcttcgat cagatgaccg atctcgcgct cgacttccgt
      841 gcctgtctgg ttgagcgcgc cgaagtccgc ctgcccgaaa tcatcacggt ccagcgcgcc
      901 agcgatggca cctgcaagtg gctgctgcgc gccgacgcgt cgcaggcgtt cgagatggtc
      961 tatatcccgg aggtggaccg cggcacgctg tgcatttcct cgcaggtcgg ctgcgcgctg
     1021 gactgcgctt tctgttccac cgcgcagcag gggttcaatc gcaacctgag caccgcggag
     1081 atcgtggggc aggtgtggct tgcgaaccgt gaactgggat tccagccagg cggcgatcgc
     1141 atcatcacca atgtcgtgct gatggggatg ggagagccgc tggcaaactt tcgcaatgtg
     1201 attgccgcgt gccggatctt cctggacgat ctggggatgg atctgtcacg acgtcgggtg
     1261 accctgtcca cctccggcct cgtgccacag atctatcgcc tggcgcagga aatcaatgtc
     1321 gctctggcgg tttccctgca tgccccggac gatgagctgc gcgaccggct ggtacccatc
     1381 aaccgcttgc acccgatcgc cgaactgctc gaggcgtgct ggcactacat cgacgaacag
     1441 aacgggcgca gcgtcacgtt cgagtatgtg atgctggagg gcgtgaacga ctcaccgctg
     1501 caggcgcgcc gcctgagtaa actgctcgct gggcacccgg ccaaagtgaa cctgattcca
     1561 ttcaatccct atccgggcgc acaattccac cgctccggcg atgaggcgat cgtgcggttt
     1621 cgcgatgagt tgaatcgggc cggggttatc gccacgatac ggcgcacgcg cggcgatgac
     1681 atcgatgcag cgtgtggaca gctcgtggga cgcgtgatcg atcgcaccgt ggtgcgtctg
     1741 ggagccaagg ccgcggcgcg cttgtcgcgc gctgccggga tgaatgcatg aaacggatgc
     1801 tgttatcggt cgtgtcaggg ctggcacttg cgagctgcca cagcactcca acggtcaatc
     1861 cgtcggttgg cagcaacgcc aatcccggac gcccaacgac ttccggcggc ccgacccaga
     1921 cgcagcgcgc ggccgccgcc cgagccaaca tgacgctggg cctggggtat ctgcagcagg
     1981 gcaacctgcc gatcgccaag gaaaagctcg ataaagcgcg cagtgaggac cctgatctgc
     2041 ccgacattca cggcgcgatg gcactgctcg atgagcgcct cggcaaagac aaggaagccg
     2101 ataaggagtt tcgcgcggcg ctgaagatcg acccacacga tccggcgatg ctcaacaact
     2161 atgcggtgtt cctgtgcagt cacggcaagc ccgatgaggg cgtgcgtaac ttcgagcagg
     2221 cagcctccaa tccgctctac tcgacaccgt gggctgccta taccaacgcc ggggtctgtc
     2281 tgcgtgcggc gcaccgcgat gccgaggccc ggccgcgctt tgatcgcgca ttgcgttcca
     2341 atccggctta cagcgaagcg gtgttccagt ccgccgatct ggatctgcag cagcagaaat
     2401 atccggaggc gcgcttcctg atcgatctgt tcctgctgcg aaatccggcc acacccgatc
     2461 tgctgctgct cgcgtggcgt gtcgcgcagg cacagaacga tccgaccgcc caacagaaat
     2521 acgcggcgcg gctggcgcag gaattcccca attcggaaca atcgcgcgcg ctcaccccca
     2581 cgcgacccaa tcctggatga accatggccg acggggaggg gacatcgctg accagcagtc
     2641 ccggagcacg acttgcagtg gcccggaggc aggccggcat gtccaccagc gaggcggccg
     2701 agcgtctgca cctgaacccg cagacactgg aggcgctgga agcagggcgc atggaggcac
     2761 tcggcgcagc cgtttatgcg cgcgggcacc tgaagcgata tgccgagctg gtgggagtcg
     2821 cggaatccga aatactggcc gcgtacgacg cctggtcagg ccgacccgca gcactgccgg
     2881 atctgcgcga ggtcattacc gcgccggcgg tacgctccgg aacgcgccgc ttcgaactca
     2941 aagccggaca cgcgctgaca ggggccattg ttctggttct ggtgatggtg gtgtggtggg
     3001 cgatgcgaaa gccttcgcgg gtgagcgagc gcgccgtgcc agcggccgcg gtgcaatcgg
     3061 ccgcgccagc cccggcggcc ccgacgctca ccccgtcctc aataccggcc ccaacacccg
     3121 ccgcaccgcc gcctgccccg gcgccgccgg tggccgcgac ggtaaggccg gtcgccccga
     3181 gcagtgccca gtccaggccg ccaccggtgg cagtctccgc gccgctggtc aatgcgcctg
     3241 cggtatccgc gcctgcaacg gtgtcggtaa gcgggccgcc ggctcgcctg gcactgaatt
     3301 tcagcgaaaa tagctggata gaaatctatg gtgccgatgg tgcacagttg tttcatggaa
     3361 tggctcaggc gggctcacaa catcgcgtca ccggcatcgc cccgctgcgg gtgtttctcg
     3421 gcaatccgcc ggccgtgacg cttgaaatga acggacatcc tgtggtcatg accggcaatc
     3481 ccaaaccgcg tcgattctcg ctcgatgatg ccggccacat cgtggaagtg cgcgcctccg
     3541 atacgtcagc caaggggccg accgcgcagc ctcgatccga ctgaccttcg gagaacatcg
     3601 ttgagcaaac agattcagcc ggttcgcggc atgaacgatg tgctgcccgc gcaaatcgcg
     3661 ctctggcagc acgtcgaacg tgtcgcccgc ggcatcttcg agtcctacgg ctaccaagaa
     3721 atacgcgtac cggttgtcga acacaccgag ctgttcaagc gctcgatcgg tgaattcact
     3781 gatatcgtcg agaaggagat gtataccttc caggacctcg gtggcgacag tctgacgctg
     3841 cggcccgagg ccaccgccgg catcgtgcgg tcggccattt ccaatgggtt gctgcgcgga
     3901 gcacgccaca agctgtggtg tatcggcccg atgttccggc acgagcgtcc gcaaaaggga
     3961 cggtaccggc agttctatca gctcgacctg gaggcgatcg gcttcccggg tccggacgtg
     4021 gacattgagc tgattgcgct gatctcacgc ctgtggcgcg aactgaagct cacgcaggta
     4081 cgcctgctga tcaactcgct cggcaccgcg gccgcgcgcg ctgtctatcg cgagcggctg
     4141 caggagtacc tgcgtttgca tgagagcgag ctggatgagg acagccgccg ccggctcgag
     4201 ggtaacccgc tgcgcgtgct ggacagcaag aatccggcga tgcgcagcgt gatcgagcgc
     4261 gcgccggtgc tgacggattt cctggattcg gagtcggccg agcactttgc gcggcttcgg
     4321 gccgggctgg acgcgctggg tgtggcgtat gaagtcaatc cgcggctggt gcgcgggctg
     4381 gactattatt cacgcaccgt gtttgagtgg gtgaccgacg ccctgggcgc gcaggatgcg
     4441 gtctgctcgg gaggtcgcta cgatggcctg accgcgcaac tggggggtga ggcgacagcg
     4501 gccatcggtt ttgcgatggg gatcgagcgc gtggtggaac tggtcagcct gtcaggcagc
     4561 acgccggtcg tcgcgacgcc cgagatctac atcgtcgcga gcggatcgcg cgcggagctc
     4621 gaggcactgc gactggccga acggctgcgc gatgcgctgc ccggacgcgg tgtgctcgtc
     4681 aatctgggcg gaggcaactt caagacccag tttcgccgtg cggacaagag cggcgcccgg
     4741 ctggcgctga ttctgggcga cgcagaactc gatcgcggtg tcgccgcggt gaaaactttg
     4801 cgcagcgagg ccggtcagat ggactgcccg ctgggcgagc tgccggcgcg tattgcgtcc
     4861 tggccttaag gttggatgga gacggtgcgt ggatgagtat ctgagtgaac gggaacaatg
     4921 ggagcgcgtg ctgcagtggc tgcgcgagca aggcccgtgg gtgctggtgt cgatcgcagt
     4981 cgtgatgacg ggctactatg gttggaatta ctggcatagc cgtgtcgagc agcgctacgt
     5041 cacggccgcc acccgttatg aggccgttct gcagtccttc cgcaatcgcg acggcaaggg
     5101 cgagccgaac ctggatgcgg gactgaagct gaccgaacag ttcatcaagg actatccatc
     5161 gaccggctac gccgatcagg caaacctgtt cgccgccggc gcgctggtgg agaaccgctc
     5221 attcgatcag gcagcgagcc gattggctca ggtgatgaat tccaccaagg atcctgagct
     5281 ggggctgatc gcgcgcttgc gactcgcgcg cgtacagctc gctcagaaca agcccgatga
     5341 ggcgctcaag accctcagtg cggtcgagcc gggtgccttt gccgcgcgtt actcgcaagt
     5401 gcgcggcgat gcgctgtttg ccaagggtga tcgcgagggt gcgctcaggg agtatcgcgc
     5461 cgcgcgctct gcggcgggcg acaccatcga taccggactg ctcgatctga agatcgacga
     5521 actggcgcac tcgtgagcac tgaacgcctg agcctgcgag gctggtcggc ggtgttgctg
     5581 gccgtgctgc tcggcgtggt gctgctaccg ggatgcagcc acaagaagga cacgaagaag
     5641 accaacgaca agccgatggc gctggtgccg gtcaccaatc acttcgaggt gcgccgcgta
     5701 tggtcgaacc gaatctccgg tgagagcccg aagctgcggc tcgggctggg agtggccgcg
     5761 agcggtgagc gcgtgttcgt cgccagtcac aagggtgtgg tggacgcgtt cgcgctggac
     5821 agcggcaagc gcctgtggcg cgtgcggttg aaagcgcccc tgtcaggggg tccgtccgcc
     5881 ggccatgaca gggtggccgt cggcacgtcg aagggcgagg tcatcctgct gtcggaaacc
     5941 gacggcagcg tactttggcg cgtgcgtgtc aactccgaga tcctggcggg tccggcgatt
     6001 ggcgccgacc ttgtcgtggt gcgcactgta gacggcaaga tgcatgggct ggcggtcaag
     6061 gacggtgtcg agagctgggt cgtcgaccag caggtgccga agctgtcact gcgcggaacc
     6121 agcgcgccga cactctcggg cgatctggcg atcaccggtt tcgacaatgg tcgcgtggtc
     6181 gcagtcaatc gccgtaacgg ttcgacggta tgggatgccg ccgtcggaca gtcacacggc
     6241 agtaccgagc tggcacgtct gatcgacgtg gactcgccgg tggtggtcga aggagaagac
     6301 ctgtttaccg tggcgttcca ggggcgcgtc gtgcacatct cgcgcgacac gggccaggtg
     6361 ctgtggacac atgacgtgtc gagctatcgc gggctggcga tcgatgccga cagcgtctat
     6421 gtctccaccg ccgacggcga catcgtacgc ctcgaccgca agaacggcac cgagcagtgg
     6481 cggcaaaagg cgatggagcg ccggcagctc accgcaccgg tcctctatga aggctatccg
     6541 gtcgtggccg acggcggcgg cgtacttcat tggctcaatc ccgcgaacgg tgatgtcgtg
     6601 gcccgcgccg aagtggacaa gtcctcctcg cgcaacgcgc tcaagtccaa gggaatctca
     6661 ctcaagcgaa gaatcagcag tgagccgatc gcggccggag gcttgctgct ggtattcacc
     6721 gactctggtc tgctcagcgc atttcgctcc ccgccgcccg gcggaaaatg agcttggccg
     6781 atgttgccga tcgttgcgat cgtcgggcgg ccgaacgtcg gcaagtccac cctctttaac
     6841 gcgctgacgc gcagtcaggc ggccatcgtg gcggacgtac ccggcgtcac ccgcgaccgg
     6901 cagtacggct actcgcgcat cggtgcggtg ccgtgtgtgc tgatcgatac cggcggcctg
     6961 atcgaaaacc ccacgggtct ggaggcgcag atgcgcgtgc agaccgagaa agcagtcgcc
     7021 gaggccgacc ggctgatcct cgtggcggac gctcgcgccg gaatgacctc acaggatcag
     7081 ttcatcgcgc gcgaactgcg ccgcaccggc aaaccggtgg tgctggcact caacaaggcc
     7141 gaaggtctcg acgaggacat ggtcgccgcg gactttcata cgctcgggtt gggcaggccg
     7201 cttgcgatcg ctgccagtca cggccagggc tgccgcgagt tgatcgacga ggtgttgagc
     7261 ggcatgcagg ccgatgcagc gcccgccgag gataccagcg cgatccgtgt cgcgatcatc
     7321 ggacgaccga acgtcggcaa gtccacgctg gtgaatcggc tgctcggtga ggagcgtgtg
     7381 attgccagcg aacagccggg aaccacgcgc gatagtatcc tggtgccgtt tcagcgcgac
     7441 ggtcgcgact tcctgctggt ggacaccgcg ggtgtgcgcc ggcgcgggaa ggtggccgat
     7501 gcggtcgagc gcgccagcgt cgcgcgcacg ctgcaggcga ttgccgaatc gcacgtggtg
     7561 atcctggtgc tggatgcgca cgatgcgatc ggtgagcagg acgcgagcgt gctcggacaa
     7621 gcgctggatc ggggccgtgt gatcctgatc gcaatcaata aatgggatgg catcgcgacg
     7681 gagcagcgtg acgagatccg ccgccagcta tccctaaaac tggatttcgt gccgttcgcg
     7741 ccgctgcatt tcatttctgc gcgtcacggc actggcgtcg gagagctggc gcgcgatgtg
     7801 atccgcggct acgacgcggc catgcgcgcg atggcgaccc cggaactgac ccgcacgctg
     7861 gagcgcgccc tgcaggcgca tcaaccgccg ctggtgcgcg gtcggcgcat caagctgcgc
     7921 tacgcacatc agggcgggcg caatccaccg cgcatcatcg tgcatggcaa tcagaccgcc
     7981 tcggtgccgg aagcctaccg acgctatctg gcgaatgtat ttcgcgagac gttcgatctg
     8041 tacgcgaccc cggtggcggt cgattttcgt accgatgcca atccgtacga tcgcaagcga
     8101 gcaggcaaag tcagggctcg gcgggcggca ggaacaatgc cgggtcgaca aaggcgtggt
     8161 tgagcgcaac cccgaagtgc agatgaggtc cggtgacacg ccccgtggcg cccaccttgc
     8221 cgatgatgcc cccggcatgc accacctcgc ccacatgcac gctgatctgc gacagatggc
     8281 agtacatcgt gatcagtcct tcgccgtgat cgagcagcac cgtgttgccg ttgaagaagt
     8341 aattcccggt atcgatgacc cgcccgtccg ccgcggcatg caccgcaagg ccttggggcg
     8401 ccgggatgtc catgcctgaa tgcggattgc gcgcctcgcc gttgaagacg cggcgcgatc
     8461 cataggaact cgagcgcgca cccgggaccg gcggcagcag gcgcagtgtc gagggcggct
     8521 gctcggagaa gctggacagc gcggtctgca gatgcgaccg ctcgcgtgcc gcgcgggcct
     8581 gatcacgccc ggacagatcc accttagctg gggagaccgt caggcgctgc gtggtgtagt
     8641 tcttgtcgcc gacctgaaag ctctggctgc ttgccggggc accggcactg gcagcgatac
     8701 tgaccgtggt gttgccgatc ggagcactga gcggaatccc gatcactgcc agccagtgat
     8761 cggcctgccg cagcaccatc acgcggttgg actcgaaggt gaccaccggg gtggcatccg
     8821 cgggctgctg cagaggcagt aacaccacgc ctccgggcac tgcttcggcc tgtggcagat
     8881 ccagcgcaaa gacgccgggc acggcactca gcgtcagtgc cgccgccagc acgcaggcac
     8941 gcacgctctt catgggccgc gttcgatcac cctggcgcgc accgaaccgt tcgcgagctg
     9001 tgcatcgagg tcctcgccca ccgacaactg ttcggcagaa gtcaccagtg cgccgtcggc
     9061 gctgcgcgtg atgaccgcga agccgcggcc cagcgtagcc agcgggctga cagcctgcag
     9121 tgcgcgcgcg gcgctgtcca gccgtgcggc ctggtgcgcc acgcagacgc gcacggcgcg
     9181 ctgcagtctc tggtcgagct gcctcagccg ctgatcatac tgcgcgagac gaattccggg
     9241 atgcgcctgc tgcaggcggt gcccgaggga gcgcaggtgg gcgacctgct gtgcgagccg
     9301 gacgcgcgcc gagcgcgaca gacgctgaga cagcgcctgc agacgctcgc catcgacgcc
     9361 gattgtgcgg cgcatgccag cgaggaagcg ccggtgaagc tgggccagcc ggtgcagcca
     9421 gtgacgggtg tcaggtgcca ccagctcggc ggctccggag ggtgtcggcg cacgcacgtc
     9481 ggcgacgaag tcggcaatcg taaagtcgat ctcatggccg atcccggcga ccaccggaat
     9541 gccgcagcgg tagatcgcac gtgccacgct ctcctcgttg aaggcccaca gatcctcgag
     9601 cgagccgcca ccgcgcgcca ggatcagaac atcgcactcg gcgcgggcgg aagccaggtc
     9661 cagcgctctg acgatcgcgc gcggcgccgc atccccttgt accgggaccg gatgcaccag
     9721 gacacgcgct gacggaaagc gccggcccag gatattcaga atgtcgcgga tggccgctcc
     9781 gctgggggag gtgatgacgc cgatcagtct gggcgccgtc ggcagcgcac gcttgcgctc
     9841 cgcggcgaac aggccttcgg cctcgagttt gcgcttgaga tcgtcgaaag cacgttgcag
     9901 ggctccgagt cccgcgtctt ccagatgctc gatctgcagc tgaaactcgc cgcgcggttc
     9961 gtacaggctg acgcggccgc gcgccagcag caactggcct tcacgcggcg tgaagcgggc
    10021 caggatattg cgctgccggt acatgcaaca gcgcagctgc gcctcgccgt ctttcagcga
    10081 aaaataccag tgacccgaag ccggccgcga tagattcgac agctcgccct ggacccagac
    10141 gttgccgatg cccgactcga tcagcagccg cacttcgcgg ttgagatccg acaccgagta
    10201 gatcgtgcgc tgtctggccg gtggctcggg agtgccggct ttggcggcgg agggcggatc
    10261 aaaaaactcg tgctgcatgg ccggaatcaa cgctaagcgg acgctttgcc caggaagtgg
    10321 tttacccaac tgcggtgaca cgataccatg cactgcttct tctcgtagcc gcgaggctcc
    10381 atgcgaatcc tcgaagaagc ccttaccttc gatgacgtcc tgctcgttcc cgcacgttcc
    10441 gacgtcctgc cgcgcgaagt tgatctgtcc actcaactga cccgccgaat ccggctcggt
    10501 ctgccagtgc tgtctgcggc catggatacg gtgaccgagg ggcgactggc gatcacgatg
    10561 gcgcaggaag gcggcatcgg catcatccac aagaacatgt cgatcgagtc gcaggcacgc
    10621 gaagtcgcgc aggtgaaaaa attcgaaagc ggcgtgatca ccgatccgat cacggtgccg
    10681 cccgagacca ccattcgtca ggtcatcgaa ctgacgcgcg cgcgcggcat ctccggtgta
    10741 ccggtggtca gcggcaacaa ggtgctcggt atcgtcacgc accgcgacct gcgctttgag
    10801 cgccagctgg atgctccggt cagcgcggtg atgacaccga agtcacgcct gatcacggtg
    10861 cgcgagaacg caccgaagga agaagtgcag cgcctgctgc acaagcatcg catcgagaaa
    10921 gtgctggtcg tcaatggcga cgacgagctc cgcggcatga tcacggtgaa ggatttccag
    10981 aaggcgaccg agttcccgcg cgccagcaag gatgtcagcg gtcggctgcg ggtcggcgcc
    11041 gcggtcggca ccagtccgga cacgctcgat cgcgtcagcg ccctgcgcga ggcgggcgtc
    11101 gatgtgatcg tcgtggacac ctcccacggt cactcgcgcg gcgtgctcga tatgattgcg
    11161 aagctcaaat cgcgctggtc agatctggaa ttgatcgccg gtaacatcgt gacgcgtgag
    11221 gcggcactgg atctggtggc ggcgggtacg gatgcggtca aggtcggtat cggacccggc
    11281 tcgatctgca ccacacgtgt ggtcgcgggc gtcggcgtgc cgcagatttc ggcggtcgca
    11341 aacgtcgctg ccgcgctgga aggcaagccg atctcgatca ttgccgacgg tggtatccgc
    11401 tattcgggtg atctggccaa ggcgatcgca gccggcgcgc acgccgtgat gatcggtggc
    11461 atgttcgccg gtaccgagga gtctcccgga gaagtcgagc tctatcaggg cgcttcctac
    11521 aaggcctacc gcggcatggg ctcgctgggt gcgatggccg agcgctacgg ctccgccgat
    11581 cgctactttc aggacaccgc ttctgaactg gagaagctgg tgcccgaggg gatcgaaggc
    11641 cgtgtgacct acaagggcag cctggtcacg attctctatc agctcgccgg cggcctgcgc
    11701 gcggcgatgg gttacaccgg cagtcacagc atcgaggaga tgcgcacccg accggtgttt
    11761 gtgcgggtga cgaccgcagg ggtgcgcgaa agccacgtgc acgacgtgac gatcaccaaa
    11821 gaggccccca attaccgcat ctcctgaagc gcgcgtgcgc gcagcgagcg aggtcgagat
    11881 actggcgacc gatattcacg cgcaccgcat cctgatcctt gatttcggtg cccagtacac
    11941 gcagctgatc gcgcggcgcg tgcgcgagct gggggtctac tgcgagattc atccgtggga
    12001 catcgatgac gccgaggtca agcgcttcgg cgcacgcggc atcatcctgt cgggcggccc
    12061 cgagtcggtc accgatgcca cgcctcccac agctgcgcag tgcgtgttcg agctgaacgt
    12121 gccggtactg ggtatctgct atggcatgca gacgatggca cagcagctcg ggggccgggt
    12181 cgccgccggt agcgtgcgcg aatttggtta tgccgaagtg caggtgagtg ccgcgtgccg
    12241 cctgctggat gcgatcagcg accgtctcag cgacggccac gcgatactcg atgtctggat
    12301 gagccatggc gatcgcgttg aagcgctgcc cgccggtttt ggggtggtcg cgagcaccca
    12361 cagcgcaccg ctggcagcga tggcggatga gcggcgtcac ttctatggcg tgcagtttca
    12421 tcccgaagtc acgcacacgc gtcagggaac cgcgatcctg gagcgcttcg tgcgtgacat
    12481 ctgcgcctgt gaggcgcgct ggaccgccag ctcgatcatc gatgatctga tcgcgcggat
    12541 acgcgcgcag gtcggcaccg accaggtgct gctcggcctg tccggtggcg tggattcttc
    12601 ggtggtcgca gcgctgctgc atcgggcgat tggcccgcag ctcacctgcg tgttcgttga
    12661 tcacggactg ctgcgcctgg gcgagggcga tcaggtgatg cgcacctttg ctgactcgct
    12721 cggcgtgcac gtgattcgca tcaatgccga actgcgcttc ctgagcgctc tgcgcggaca
    12781 gaccgatccg gaggccaagc gcaagatcat cgggaggctc ttcatcgagg tgttcgagca
    12841 ggaagcccgc aagctgcgcg acacgcgctt cctggcgcag ggcacgatct acccggatgt
    12901 catcgaatcg gccggcgcac gcaccggcaa ggcccacgtc atcaagtcac accacaacgt
    12961 cggtggcctg ccagcggatc tgaagttgaa actggtcgag ccgttgcgcg agctgttcaa
    13021 ggacgaggtg cgccgcatcg gggtcgagct gggattgccg cacgacctgg ttcaccggca
    13081 tccatttccg ggcccgggac tggcggtgcg aatcctcggc gaggtgaagc acgaatacgc
    13141 cgagctgctg cgtcgcgccg atcacatctt catcgaggaa ctgcgtcgcc attctctcta
    13201 tgaacgcacc agtcaggcgt tcgccgtgtt cctgccggtg cgatcggtcg gcgtgatggg
    13261 cgacggccgg cgctacgact atgtgattgc tttgcgcgcc gtcgagacga tcgatttcat
    13321 gacggcacac tggtcccatc tgccgtacga attcctcgac ctggtctcgc gccggatcgt
    13381 caacgaagtg ccgggcatct cgcgcgtggt ctacgacatt tcgggaaagc cgccggcgac
    13441 gatagagtgg gagtaagcgg accttcggct caagctacta ttaagaacct ggggtggaga
    13501 ttttgtacca tggcaaagct cgtctatgga ttgaaccagt ccttggatgg ctacgtcgac
    13561 catctggaat tcaggccaag cctggcgctc tttcgtcact tcatagagca tgtgcgcgac
    13621 ctgacgggca ttgtgtacgg tcgtcgcatg tacgaggtaa tgcgttattg ggacgaagac
    13681 cttgcggact gggtcgcgga ggaacacgat ttcgcggcgg cgtggcgcag ccagccgaag
    13741 tgggttgtgt cgcgctcgtt gaagtcggtc ggacccaacg ccacacttgt cgagaatgac
    13801 atcgaggcgg tgatccgtgg gctgaaggct cggccagttg ggcagattga agttgccgga
    13861 ccagacttgg caggaagcct aaccaacctg ggtcttatcg atgagtatcg actttacctc
    13921 caccccgtcg tgcttggccg cggcaagcca ttcttcgccg gcccccggcc gccgctgcgc
    13981 cttgtgacca gcgatcgaat agccgaggac gtgattaggt tgacgtacgt gcccgcttaa
    14041 tcgcgcacgt atcctgggat atcacccgtg tgctcgaggc gaacaaacgt gattcgatcg
    14101 cctgcgacca cgggactact ttcaatggtt ctgagtacct cgtagtcgtg atcaggtgtc
    14161 agcgctacga gaggtaggag gccatgcgcc tctgcaggcc aaagtgacgg ttccttgcct
    14221 actccggaga tggcggcgat cactcgaatg gattcaagct ctgcacgtca aaacgggaaa
    14281 aatctcgcat gttgcgcgtt accacggtca gatcgtgcac gagtgctgtt gccgcaatca
    14341 tggcgtcttc atagacgcta ctcgattggc gatgcatgag cttggcccat cgtcgaaagc
    14401 acgcagcgtc catcacgagt acattgaacg aatctgccaa ttgatcagcc catgcctcga
    14461 gttccgctgc cttggtaggg tcctgcgagc gcgtcacctc gatgccggct tggatctctc
    14521 cgatcgttac agcggaaaga tgtagatcgg ggtccttcac agcctgaacc caagcgacga
    14581 ctttcggatt tgggcggcgc ttgcgcagct ccgatactat gttggtgtcg agtaagtaca
    14641 cggggtaagt cagagcgacg gaattgggcg tctccgatgc cctccacgtg gcggtattgc
    14701 cagctcggta cgcgcgaaac ccgtcaacag gagatctttc aacgacggcc gtgcaacttg
    14761 gcacagtcgc ttccactcat taattggcac tagcaccgcc gtttcttctc cgcggcgcgt
    14821 gacgatttgc ggcccttcac ggacgcatgt ctccaaaagt tcgctgaagc gtgctttggc
    14881 gtcctgtaca ggccagctct ttgtcttcat aagccaatgt tatgactagt tatctgacta
    14941 gtcaagttac gcaggctatc gccgactctc caacaggttc ctaatgacct gcccgacagc
    15001 acccatttgc gaacagcagc aagggatcaa tcggtcgcct tgtgccgcgg acgcacacta
    15061 gctatcccag tcactgctgg agtcagccac cggatccctt agcattgctt gtgctcgtcg
    15121 ccgagccctg gaacaagggc aagctggtcg gtcagaaggc cccgctcaaa ctgaaagaaa
    15181 tctgggccat ccgcgcatcc gcgtgcgttt acaactcgcc gatcgacgcc gcgaactcgc
    15241 gctgttcaac ctagccatcg acagtaagtt gcgggcgtgt gaccccgtga aattgcgtgt
    15301 gcgcgatgtc tgccacgctc aagtggtcgc aacgcgcgcc atctgtccac caggcagtgc
    15361 gcccggatcg tcggcgcatg ggtcaagcag attggactga acagggcgtg ttacgaaacg
    15421 catacgcttc gacggaccaa ggccacgctg atctatcgcc gctcaaaaga acctcagagc
    15481 tgtgcagcta ttgctagggc accagggtag gccacgaagc tcttgcgcta gggctttaag
    15541 ttcttcttcg tgagaatagt tgggcggtcg atctgaagcg cccgcggcat cgaaagcctt
    15601 acccattccg accctatttc cagatgcatc gaacgcgatc ccaaccactt gcgcaggcag
    15661 gattttgagg gcatccagcc atatgcaatg cacatctgca ctgccttggc cgtaagttcc
    15721 tccccacgcc gataggtcag cgtgatcgtc atcgtcgcga ctgccgccga tgcgcgcacc
    15781 aaggatattg catccctcag tgggcagagc agcaacaacc gttccgtcaa tctggacgcg
    15841 taatagaggc atccgccgcc gacctaataa ctggaactcg gacttagatt actgcaggcc
    15901 atgaacgact gcttagaggc atccaatcca gtgtgcgcta ctaccgcttt tggccggttg
    15961 tactcgttcg ccgacgaaag gccggcattt ccagtccaac gggtcgtcat gacactctgc
    16021 ggacgctcac aagtgaccgc tcacgcgaca gcaaatgctt cgctttcttc agctggcgcc
    16081 caacgccaga gacgaggcta ccacggccag tgataaggcc cccccagtcc atcgatacca
    16141 tatagcgttt ctgcgacgtc atactggtaa aagattgatc ccgcgcctac aacccaacaa
    16201 attgctgcgg ccaacagcca ccaagaggca aagctgcggc ggcctagaag cagcacgcag
    16261 gccaaggcag gcgcagccga aaacgttagg gcgacatgaa gcaagaaata tagcggccca
    16321 atcaatgcgc gcgcccccaa tacgcctcgg atcaatacga tcagtacctc cacgcaataa
    16381 gtgatcatca gcggaacggc aaccgtgaag atcagcgccg acaagcgagg gaagtggcgc
    16441 accaccttgg ccagaaaaaa gcagaacacg gg
//
LOCUS       DATMRM010000214         4104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3518649, whole genome shotgun sequence.
ACCESSION   DATMRM010000214 DATMRM010000000
VERSION     DATMRM010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3518649"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VNZ06_06205"
     CDS             <1..575
                     /locus_tag="VNZ06_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HWW20378.1"
                     /translation="EEQIQTELARLTQHELEAMVAEARNEYRVEVDRRYRILSLADSP
                     ANSHLWTHYADNYAGICLRFFVNPLFGTAYRMRYSDTLPALDLTDDDGLSALNVTALV
                     KRTRWATEGEYRMIFGEPPIPGDLPLEAQKLRFAPDLLTGLLFGHRVPAAHRARLERA
                     FLAAPRAREVFTVTGGVPFQQLSIIRSRRT"
     gene            572..1171
                     /locus_tag="VNZ06_06210"
     CDS             572..1171
                     /locus_tag="VNZ06_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20379.1"
                     /translation="MTLDEARSSVSRLAHQLANGYYAFVVAHYVAVNPLSINALPPAK
                     EPGQNPTGQLPRKDGTFHTIDLRHYLDLLREDFDLQGHVLRAWATGALLTLGDALDKD
                     KRNYFDRAPVLELVYHLRNGIAHGNRFTIKDSGKKRLANYPAHNGQAAVRSPGAVFEI
                     TSETTGPVLFDFLGPADVIDLLQSVEIHLSKPAPAGHSG"
     gene            complement(1439..3100)
                     /locus_tag="VNZ06_06215"
     CDS             complement(1439..3100)
                     /locus_tag="VNZ06_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HWW20380.1"
                     /translation="MTLTFTLIDLAGSVALLLWGTHMVQTGIQRAFGATLRSLLGRAL
                     RGRLGAFLAGIGVTAVLQSSTATGLMTAGFAAGGLVDLVPALAVMLGANVGTTLIVQV
                     LSFDVAAASPAVILVGVLMFRKASNTRAHDLGRVLIGLGLMLLALHQLLGLMTDYEDA
                     PSLRMLLGAASTVPLVDVLLAAGLTWAAHSSVAIILLIMSLCAQNVVPPDAAFALVLG
                     ANLGTAINPLLEGATAGDPASKRLPVGNLLSRAVGVVMALAALGPLGRLMVTIEPDNA
                     RVVADFHTMFNLVLACLFLPLLSPYARLLGRLLPQRADPADPSRPLYLDPAARQIPMV
                     ALGNAAREALRLADVLGEMLAGARAALVEGDRKLIVETRRRDDILDNLNTAIKTYLTS
                     LDPEQLAENDHRRLHEILTFVINIEQAGDVVDLNLLPHATKRVKRGLAFSKEGETELL
                     AMMDRLMTNLRTAASLFMTEDPRTARMLADEKVAFREAESTATASHFERLRSGRIDTA
                     QTSALHLDLLRDLKLINSHIVAAAAYPVLERTGALLPSRIAANEM"
     gene            complement(3297..3722)
                     /locus_tag="VNZ06_06220"
     CDS             complement(3297..3722)
                     /locus_tag="VNZ06_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20381.1"
                     /translation="MKPQATRNRSGKRPYVHTAAGANEAAAGKEPVDPTKVRRSATKA
                     KTRKATPVEEPPPRVSRHRREHVKREKVVRDTFTMPRADYEKIAALKQRCLDAGVAVK
                     KSELVRAGLLLLESEPTKRLLAAVTAVEPVKTGRPPKSK"
     gene            3788..>4104
                     /locus_tag="VNZ06_06225"
     CDS             3788..>4104
                     /locus_tag="VNZ06_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HWW20382.1"
                     /translation="MPDKHISSGFDADLEHLSLRFFEMGGLVESQLTDAMEALSRFDM
                     GLVEKVVDEEHRLNAMEVEIDAECGNIIARRQPAASDLRRLMAISKGITNLERAGDEA
                     RKIA"
BASE COUNT          767 a         1310 c         1271 g          756 t
ORIGIN      
        1 gcgaagaaca gatccagacc gaactcgcac ggctgacgca gcatgagctt gaggcgatgg
       61 ttgccgaagc gcgcaacgaa taccgcgtcg aggtcgacag gcgctaccgg atcctgagcc
      121 ttgcggattc gcctgcgaac agccatctgt ggacacacta tgccgacaat tacgcaggta
      181 tctgtctacg gttcttcgtg aatccgctct tcggtaccgc gtaccggatg cgctacagcg
      241 acacgctgcc tgcgctcgac ctaaccgacg atgacggatt aagcgccctg aatgtgacag
      301 ccctggtgaa acgcacacgc tgggcaaccg agggggaata ccggatgatc tttggtgagc
      361 cacccattcc cggtgatctg ccgctcgaag cgcagaagct gcgattcgcg cctgatctgc
      421 tgaccggcct cctgttcggc catcgtgtgc cagccgctca ccgggcgcgg ctggagcgtg
      481 cctttctcgc ggcacccagg gcgcgcgagg tctttacggt gaccggcggt gtgccattcc
      541 agcagctctc gatcatcagg agccgtcgca catgaccctt gatgaagcga gatcgtccgt
      601 ctccaggctg gcccaccagc tggcgaacgg ctactacgcc tttgtggtcg cgcactatgt
      661 cgcagtcaat ccgctgtcca tcaatgcgct gccgcccgcg aaggaacccg gacagaaccc
      721 cacaggtcag ctcccgcgga aggacggcac ctttcacacg atcgatcttc gccactacct
      781 tgatctgctg cgcgaagatt tcgacctgca ggggcatgtt cttcgcgctt gggcgacggg
      841 cgccttgctc acgctgggtg atgcgctcga caaggacaag cgcaactact ttgaccgggc
      901 gccggtgctc gagctggttt atcacctgcg caatggcatt gcgcatggca accgcttcac
      961 gatcaaagat agcggaaaaa agcggctggc aaattacccg gctcataacg gacaggcggc
     1021 ggtgcgaagc ccgggtgcgg ttttcgagat cacgtccgag acgacggggc cagtgctgtt
     1081 tgacttcctc ggaccggcag acgtgatcga tctgcttcag tcagtggaaa tccacctttc
     1141 gaagccggcc cccgcaggcc actcgggata gctggcatgt cgaccatcgc gacggacgcg
     1201 taaccgtgtt ggccatcacc gcctggcaaa gctgctcgac gaagcggtga ttgcgaccga
     1261 tataatgaaa gccctcggac agcgctaaag ggaactcgcc ggttcaacgg gttcgtcggt
     1321 gccgacctta tagagtgtcg tcgcgaggcc gaatcttgcg ctcgcgatgc cctcatgttg
     1381 gctcgttttc cccgcggact gtctgcatac agtgcagcgt gagcccactt ttttcggact
     1441 acatttcgtt cgcggcaatc cggctgggca acagggctcc cgtgcgctcg aggaccgggt
     1501 acgcggctgc ggcgacgata tgcgaattga tgagcttcag gtcacggagc agatcgaggt
     1561 gcagtgcgct ggtctgggcg gtgtcaatgc gaccggagcg cagccgctca aagtgcgaag
     1621 ccgtggcagt ggattccgct tccctgaagg ccaccttctc gtcagccagc atgcgtgccg
     1681 tacgtggatc ctccgtcatg aacagcgacg cggcggtacg cagattggtc atcaggcggt
     1741 ccatcatggc cagcagttcc gtttcccctt ccttcgaaaa cgcgagtccg cgcttcacgc
     1801 gcttggtggc gtgcgggagc aggttcagat ccaccacgtc gccagcctgc tcgatgttga
     1861 taacgaaggt caggatctcg tgcagccgcc gatggtcgtt ctcagcaagt tgttcgggat
     1921 cgagcgacgt caggtacgtc ttgatcgcgg tattcaggtt gtccaggatg tcatcgcgcc
     1981 ggcgggtttc cacaatcagt ttccggtcgc cctccaccag cgccgcgcgg gcacccgcca
     2041 gcatctcgcc gagcacatcg gccaggcgca gtgcctcacg tgccgcattg ccaagcgcca
     2101 ccatcggaat ctgccgtgca gccggatcca gatacagtgg ccgcgacggg tcggcgggat
     2161 ctgcgcgctg aggcagcagc cggcccagca gtcgcgcgta cggagacagc aacggcagga
     2221 acaggcaggc aagcacaagg ttgaacatcg tatgaaaatc ggccaccacc cgggcattgt
     2281 ccggttcgat cgtgaccatg agccggccaa gcggcccgag cgcggcgagc gccatcacga
     2341 cgcccacggc ccgcgagagc agattgccca ccggcaaccg cttcgacgcc ggatcacccg
     2401 cggtggcccc ctccagcagc gggttgattg cagtgccgag attcgcgccc agcaccagtg
     2461 cgaatgcggc atcgggcggc accacgttct gcgcacacag cgacatgatg agcaggatga
     2521 tcgccacgct tgaatgcgcg gcccaggtca gacccgctgc cagcagcaca tccaccagcg
     2581 gcacggtgga tgcggcgccc agcagcatgc gcagactggg cgcgtcctcg tagtccgtca
     2641 tcagcccgag caactggtgc agcgcgagca gcatcaggcc caggccaatc aggacgcggc
     2701 ccagatcgtg tgcccgtgtg tttgacgcct tccggaacat cagcacgccg acgagtatca
     2761 ccgccggcga ggccgctgca acatcgaacg acagcacctg cacgatcagt gtcgtgccga
     2821 cgttcgcgcc gagcatgacg gcgagcgcgg ggaccagatc gaccaggccg ccggcggcga
     2881 aaccggccgt catgagtccg gtcgccgtac tgctctgcaa taccgcggtg acgccgatcc
     2941 cggccaggaa ggcgcccagt cgcccgcgta gagcccgacc gagcagtgag cgaagggtgg
     3001 caccgaacgc gcgctggatg ccggtctgca ccatgtgggt accccacagg agcagcgcaa
     3061 cggatccagc cagatcgatc agcgtgaacg tcaatgtcat gattcagagc cctccgtgcc
     3121 gaacgactgg tcatgtgact cttcgttcgg gcgtgtcgcg ccttgcctgc acattcgcat
     3181 cggacctgaa ccattaccgg ctgagccccg aagcccggac agggcaagcg cgaggtcctc
     3241 tcgcaagccc cggcttttgc cgctataggg cgcgactatc aggccccgcg cgcggctcac
     3301 ttcgattttg gtggccggcc agtcttgacc ggctcgaccg ccgtgacggc cgcgagaagg
     3361 cgtttcgttg gttcagactc cagcagaagc aggcccgccc gcacgagctc gctctttttc
     3421 acggcgacgc ccgcatcgag gcaccgctgt ttgagcgccg cgatcttttc gtagtccgcc
     3481 ctcggcatcg tgaaggtgtc ccgcacgacc ttttcccgtt tgacgtgctc cctgcggtgc
     3541 cgggagacgc gcgggggagg ctcttcaacg ggcgttgcct tccgggtctt cgccttcgtc
     3601 gccgaacgtc gaaccttcgt cggatcaacc ggctccttgc ccgcagctgc ctcattggca
     3661 cctgcagccg tgtgcacata cggtcgtttg ccgcttctgt tcctggtggc ctgtggcttc
     3721 atggttttgc gcaggtttga caaaccgtac aaacagttta tcttctttcg ggcaagggga
     3781 gttttcaatg ccagacaagc acatttccag cgggttcgac gctgaccttg aacacctgtc
     3841 gttgaggttt ttcgagatgg ggggactggt tgaatcacag ttgaccgatg cgatggaggc
     3901 gctcagccgc ttcgacatgg gcctggtcga gaaggtggtt gatgaggagc atcgactcaa
     3961 tgcgatggaa gtcgagatcg atgccgagtg cggcaacatc atcgcgcggc gccagcccgc
     4021 cgcatccgac ctgcgtcgac tgatggccat ctcaaagggc attaccaatc tcgagcgtgc
     4081 cggcgatgag gcacgcaaga tcgc
//
LOCUS       DATMRM010000215         9163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2604741, whole genome shotgun sequence.
ACCESSION   DATMRM010000215 DATMRM010000000
VERSION     DATMRM010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9163
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2604741"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VNZ06_06230"
     CDS             complement(<1..1022)
                     /locus_tag="VNZ06_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW20383.1"
                     /translation="MNPSRPYILRPVATSLLMAAILIVGAIGYLFLPLSALPEVDYPT
                     IQVQTFLPGASPEVMTSTVSAPLERQFGEMPGLKEMSSRSSAGASVITLQFDLSLNID
                     IAEQEVQAAINAAANLLPQSLPAPPIYAKVNPADAPILTLALSSSSMPITQIEDIADT
                     RVAQKISQLSGVGLVSINGAQRPAVRIQANLQALAAYGMNIDDLRTTISNANSNAPKG
                     SFDGAARSYSIDANDQISSADQYKDIIVAYRNNGPVRLSDVATVTQSAENNKLSSWMN
                     STPAILLNVQRQPGANVIAVVNQIRHILPDIHTSMPANVDITVLTDRTNTIRASVSDV
                     EFELGLA"
     gene            complement(1035..2384)
                     /locus_tag="VNZ06_06235"
     CDS             complement(1035..2384)
                     /locus_tag="VNZ06_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWW20384.1"
                     /translation="MNPQESQTGGTAGPIDAPSTRRRYLIWSAIGLVVVAAIILFARW
                     RAGAQVLPAGGRFAGAALTVGVAKVTTGDVPITINALGTVTPLATVTVHPQVTGPLVK
                     IAFTEGQTIKAGDLLVQIDPRPFQASLDQAAGQLKRDQALLANAKVDSERYKTLLAQN
                     SVSDQTYATQVATVAQDEAVVASDQAALEAAQLNLSYTRITSPVTGLVGLRQVDVGNL
                     MQANSTSIVLVTQVQPMSVLFTLPEDSLGNILQRLREGQKLMVDAYDRSLSSKIASGV
                     LSNADNEIDPTTGTLKLRAMFDNSRFELFPNQFVNVRLLVDTIHNAIVVPGAAMQQGA
                     SGSYVYVVADNSTVKMRAVKTGPSNGDQMSVTDGLQPGETVVVDGADQLRDGARVILP
                     GAAPPDSSAFGQGQRARRRPGADGSSSGGFPGAGTSSGGHFRRPQGASSGASPSSGQ"
     gene            3165..4622
                     /gene="zwf"
                     /locus_tag="VNZ06_06240"
     CDS             3165..4622
                     /gene="zwf"
                     /locus_tag="VNZ06_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWW20385.1"
                     /translation="MKQLPDFDLVLFGGTGDLAMRKLLPALFRRFLAGQFPQHARIVG
                     VARAKLEREEYVAQVEQACKKYIPTGVDAARWTEFTHTLDYTPVDANNSQGFERLAAK
                     LAQQSASVRVFFLSMAPALFEMTCTNLARAKLVTPGSRVVLEKPLGQDRASAEEINRN
                     VGAIFTEPQIYRIDHYLGKEAVQNLLALRFGNSLFEPLWGRGRIRHVQITVSEQLGVE
                     GRGAFYDQTGAMRDMIQNHMLQLLCIMAMEPPVSNDPNAIRDEKLKVLHALRPLAGND
                     VATNVVRGQYRAGASKGAPVVGYLEEKGIPADSPTETFVALKAQIDTWRWAGVPFYLR
                     TGKRLQEQIAEIMIAFEDVPHALFERAPNAGPNRMVISLQPHDSITLTILAKNPGETM
                     RLRPVDLRLDMDESFKTPQLDAYERLLTDVIKGNLTLFMRRDELDAAWRWVDPIREAW
                     ARSDETPRGYPAGSWGPAASSSLIARDGYAWNGEF"
     gene            4612..5316
                     /gene="pgl"
                     /locus_tag="VNZ06_06245"
     CDS             4612..5316
                     /gene="pgl"
                     /locus_tag="VNZ06_06245"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HWW20386.1"
                     /translation="MSFSPQERRFPHVEALNEALAADMASALEEGLGKGRGASLVVAG
                     GRTPAPAFEALSNIELDWEDVWVTLTDERWVDTHMEGSNEHLLHQHLLRKEAAKANLV
                     GLKNKFPDPASGAAAAWSAVSEIPRPFDFVLLGMGDDGHMASLFPNSPGLEKGLDLQQ
                     PPGCIAAVGPAEPRARLSLNLRALVDARRIAVLIVGASKWGTYQRARAPGPVTEMPVR
                     ALSLQQNVPVTVYWSP"
     gene            complement(5313..5798)
                     /locus_tag="VNZ06_06250"
     CDS             complement(5313..5798)
                     /locus_tag="VNZ06_06250"
                     /inference="COORDINATES: protein motif:HMM:NF016985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HWW20387.1"
                     /translation="MSVSRDDVRQHLSRILAEEAQLLAELESVLERETDILVGDDAQA
                     IQNIGSNRHRCVDALSRLGVERTDTGRMLCFGADATGLDQLFEWADPSKALHARWSVN
                     LELARRCKAINDKNGAIVAAKLNRVQKLLGQLRGVNPAPVYSSRASRYASLGLRNLGV
                     A"
     gene            complement(5795..6127)
                     /gene="flgM"
                     /locus_tag="VNZ06_06255"
     CDS             complement(5795..6127)
                     /gene="flgM"
                     /locus_tag="VNZ06_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HWW20388.1"
                     /translation="MANRIKGLDGGSIGPDSSNPIEKIRITTPVNSGSSGAPASTPRS
                     DSVHITDSARTLASLSQAVNDSPDVDMNRVSAVQQALATGVYRINPERIANNMLALEQ
                     DLGSAKQK"
     gene            complement(6201..6899)
                     /gene="flgA"
                     /locus_tag="VNZ06_06260"
     CDS             complement(6201..6899)
                     /gene="flgA"
                     /locus_tag="VNZ06_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HWW20389.1"
                     /translation="MSTLRIILYAGLFAAASTAGAMNTEDPALIRAAAERAVRSEAGT
                     AAGVLTLQVAPLDSRLRIASCDRQLTAFMTGNSSVRSQTTVGVRCEGSIRWTIYTSVN
                     VQSQAKVLIARRALTRDAEATAADFRIEDRLVPGLASAYVCDPSALAGQRLGHPMAAG
                     DALTLETLAPANLIHRGQQVTLLAHAGGFEVRMVGVALSDGHESERIKVQNQSSQRVV
                     EGIVRSSNEVEIPL"
     gene            7166..7603
                     /gene="flgB"
                     /locus_tag="VNZ06_06265"
     CDS             7166..7603
                     /gene="flgB"
                     /locus_tag="VNZ06_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HWW20390.1"
                     /translation="MRTTLDNYLGIHAQALTIEAKRNELLAANLANVDTPNYKARDID
                     FKAALSAASGNSAANGSSSLSLQQGNASLKATSATPTTGTENSGDASLMRYRVPMAPS
                     LDGNTVDEQLEQSAFAENSVRYQATLTFLNNALRGLMTAITGS"
     gene            7634..8050
                     /gene="flgC"
                     /locus_tag="VNZ06_06270"
     CDS             7634..8050
                     /gene="flgC"
                     /locus_tag="VNZ06_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HWW20391.1"
                     /translation="MSSFKIFEIAGSGMSAQSVRLNTVASNLANADSVSGDPNTVYQA
                     KHPVFQAVMGSGAGLNAASASVSVTGIAQSRAAPEMRYDPGNPLADAQGNVYAPNVNV
                     VEEMADMISASRSYQNNVEVLNTTRDMMLQTLKLGS"
     gene            8074..8766
                     /locus_tag="VNZ06_06275"
     CDS             8074..8766
                     /locus_tag="VNZ06_06275"
                     /inference="COORDINATES: protein motif:HMM:NF025230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HWW20392.1"
                     /translation="MTSPVGSPTMVNPSGTAGSSGSSSASGASSSIGGLTMNDFLTLM
                     TAQLQNQDPLNPTDSNQFLTQLSELSTVEGISQLNTSMSTLSNSMLSSQALTAASLVG
                     QGVLAPATSAFYTSGTAMTGAVQVPSGATNVVLSITNAGGALVDQLNVPASAGLQSFS
                     WNGTDANGQALPSGTYSISAAAQVGGSSQAASTLINGTVTSVTLGATGSSPTLNTLQL
                     GPVPLSSVQQID"
     gene            8787..>9163
                     /locus_tag="VNZ06_06280"
     CDS             8787..>9163
                     /locus_tag="VNZ06_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HWW20393.1"
                     /translation="MPFGIALSGLDAAQSDLNVTANNIANSQTTGFKSSNANFSELFA
                     NSSSASSTQIGNGVQLQDVEQEFTQGDINTTGNSLDLALSGNGFFAVSQGGATQFTRA
                     GSFHTDNAGNVVNAANQNLQVYSP"
BASE COUNT         1734 a         2798 c         2872 g         1759 t
ORIGIN      
        1 gccagtccca gttcgaattc gacgtcgctg accgaggcgc gaatggtgtt ggtgcgatcg
       61 gtcagcacgg tgatatcgac gttcgccggc atcgaggtgt ggatgtcggg caggatatgc
      121 cggatctggt tcaccaccgc gatcacgttc gctcccggct gacgttgtac gttcagcagg
      181 atggcgggtg tcgaattcat ccagctcgag agcttgttgt tttcggcgct ctgcgtgacc
      241 gtggcgacat cggacaggcg caccgggccg ttgttgcggt atgcgacgat gatgtctttg
      301 tactgatcgg cgctggagat ctggtcgttg gcgtcgatcg agtaggagcg tgccgcgcca
      361 tcgaaactgc ccttcggcgc attcgaattg gcattcgaaa tggtcgtgcg cagatcgtcg
      421 atattcatgc cgtaggcggc cagcgcctgc agattggcct ggattcgcac cgccgggcgc
      481 tgcgcgccat tgatactcac caggcccacc cccgagagct gtgagatctt ttgcgcgacg
      541 cgcgtatcgg ctatgtcctc gatctgcgtg atcggcatcg agctcgacga cagtgcgagc
      601 gtcaggatcg gcgcgtcagc cggattgacc ttggcgtaga tcggcggagc gggcaggctc
      661 tgtggtaaca ggttcgcggc cgcgttgatc gccgcctgga cttcctgttc ggcaatgtcg
      721 atattcagac tgaggtcaaa ctgcagcgta atgaccgagg cgccggccga actgcgcgag
      781 gacatctcct tcaagccagg catttcgccg aactgccgct ccagcggtgc actgaccgtc
      841 gaggtcatga cttcggggct ggcaccgggc aggaaggtct gcacctgaat cgtcggatag
      901 tccacctccg gcagtgcgga cagtggtagg aacagatagc cgatagcccc cacgatcagg
      961 atcgccgcca tcagcagcga ggtcgcgacc ggtcttaaga tgtaggggcg tgacgggttc
     1021 atgcgtcaac gcgcttactg tcccgatgag ggtgacgcgc ccgaactggc gccctgcgga
     1081 cggcggaagt ggccgccact ggaggtccca gcgcccggga aaccgccgct gctgctgccg
     1141 tccgcgccgg gacgacgacg agcgcgctgg ccctggccga aagccgagct gtctggcggc
     1201 gcagcgccag gcaggatgac gcgtgcgcca tcgcgtagct gatcggcccc gtcgacaacg
     1261 accgtctcac ccggctgcag gccgtcggtc accgacatct gatcgccgtt cgatggtccg
     1321 gttttgacgg cccgcatctt cacggtgctg ttgtccgcca cgacgtagac gtagctcccc
     1381 gaggcgccct gttgcatggc cgcgcccggc accacgatgg cgttgtgaat ggtgtcgacc
     1441 agcagccgca cgttcacaaa ctggttcggg aacagttcga acctggagtt atcgaacatg
     1501 gcgcgcagct tcagcgtacc ggtggtcggg tcgatctcgt tgtcggcgtt ggacaacacc
     1561 ccgcttgcaa tcttgctgct gagactgcgg tcgtaagcat ccaccatgag tttctgtccc
     1621 tcgcgcagcc gttgcaggat gttgccgagg ctatcttcag gcagtgtgaa caacaccgac
     1681 atcggttgca cttgcgtgac gagcacaatc gaggtcgagt tcgcctgcat cagattgccg
     1741 acgtcgacct gccgcaaacc taccagtccc gtgaccggcg acgtgatgcg ggtgtaggaa
     1801 agattcagtt gcgcggcctc gagtgcggcc tgatccgagg caaccacggc ctcatcctgt
     1861 gcgaccgtcg cgacctgggt cgcgtaggtc tgatccgaga ccgaattctg cgccagcagt
     1921 gtcttgtagc gctcgctgtc caccttcgca ttggcgagca acgcctgatc acgcttgagc
     1981 tgacctgccg cctgatcaag cgatgcctgg aacggacgcg gatcgatctg caccagcaga
     2041 tccccggcct tgatggtctg cccttccgtg aacgcgatct tgacgagcgg gccggtcacc
     2101 tgcgggtgca ctgtcaccgt cgccagcggg gtcacggtgc cgagcgcatt gatcgtgatc
     2161 ggtacgtcgc cagtggttac cttggccaca ccgaccgtga gtgcagcccc cgcaaagcgg
     2221 ccacctgcgg gcagcacctg cgcacccgcg cgccagcgcg cgaacaggat gatcgcagcg
     2281 accaccacga gacctatcgc cgaccagatc aaatagcggc gtcgcgtcga gggcgcatcg
     2341 attggacctg cggtaccgcc ggtctgtgat tcttgggggt tcatgtgtta ttccgtctgg
     2401 accagggcag cgcgcgatag ttaaccgaag caaatcgcaa gtagacagtt agttaacaag
     2461 atttaatttc atatcccgca ggtcccaacg aacgtgtggc gaacaattcg caacagtatg
     2521 aaacgcggga ctgcaacgat gcgtatgtta gcggtatcgg aagcgaggct ataggtcttt
     2581 tgacaaatag gttgatgaca tcgatgcgca cagtcagtgg cttggtgccc tcaatgtgaa
     2641 acgcatgact gagctcgaag ttgatctggg cataggaagg cagctgcgcg ccggtgttca
     2701 ttgcatcgct cgtgctcgat gccgacatcg aagtagttgg accgctcagc cgcgggtgcc
     2761 gtgtccagca gcactgcggg ttcggcgcta ctgccgatga actgggagcg atacggcaga
     2821 tccttgtgat cgccgcgtcc atgggctcgg tgggtgccgg cgtcgatgcc agcgaggcag
     2881 cagagggggt cggacgcctt gctggctatc gaggccgcat gcccaggacg ggatcagcgt
     2941 gctgcccgcg aacagcgcga cgctcacctc aaggcagctc gggtggcccc gacggcggtc
     3001 gatcggcctt ctgcggggac agtggcgagg aggtcataga gggaattgag taaatccggc
     3061 tcgattgtag ctcaattgcc ggctagcatt ggccttcgat cggcgcacac tcatcaaata
     3121 accggggatt agacgtgtcg aatggcaaag accgctctgt cgcagtgaag cagctccctg
     3181 atttcgatct ggtcctgttt ggaggcaccg gtgatctggc catgcgcaaa ctactgccgg
     3241 cgctattccg acgcttcctg gcagggcagt tcccccagca tgcgcgcatc gtcggcgtgg
     3301 ctcgcgccaa actcgagcgt gaggagtatg tcgcgcaggt cgaacaggct tgcaagaagt
     3361 acatacccac cggggtagac gccgctcgct ggaccgagtt cactcatacg ttggattaca
     3421 caccagtgga tgccaacaac tcgcaagggt tcgagcggct ggcagcgaag ctggcgcagc
     3481 agagcgcttc ggtgcgggtg ttcttcctgt cgatggcacc tgcgctgttc gagatgacgt
     3541 gtaccaatct ggcgcgcgcc aagctggtca cgcccggcag ccgcgttgtg ctggagaagc
     3601 cgctcggtca ggatcgcgcc tccgccgagg agatcaaccg caacgtcggc gcgatcttta
     3661 ccgaaccgca gatctatcgt atcgatcact acctcggcaa ggaagcggtc cagaacctgc
     3721 tggcgttgcg ctttggcaat tcactgttcg aacccttgtg gggacgtggc cgaatccgtc
     3781 acgtgcagat cacggtcagc gagcagctcg gtgtcgaagg acgcggagcg ttctacgatc
     3841 agaccggtgc catgcgcgac atgattcaga atcacatgct gcaactgctt tgcatcatgg
     3901 cgatggaacc gccggtgtcg aacgatccca atgcgatccg tgacgagaag ctcaaggttc
     3961 tgcacgcgct gcgcccgctg gcgggcaatg acgtcgccac caacgtcgtg cgtggccagt
     4021 accgcgccgg cgcttcgaag ggcgcgccgg tggttggata cctcgaagag aaggggattc
     4081 ctgccgacag tcctaccgag acgttcgttg cactgaaagc tcagatcgat acctggcgct
     4141 gggcgggcgt gccgttctat ctacgcaccg gcaagcgcct gcaggaacag atcgccgaga
     4201 tcatgattgc gttcgaggac gtaccgcacg cgctgttcga acgcgcaccc aatgccggac
     4261 cgaaccgcat ggtgatttcg ttgcagccgc atgattcgat cacgctgacg attctggcta
     4321 agaatccagg cgagacgatg cggttgcgtc cggtcgacct gagactcgat atggatgaga
     4381 gcttcaagac gccgcagctc gacgcctacg agcgattgct gaccgatgtg atcaagggca
     4441 atctcacttt gttcatgcga cgtgatgagc tcgatgcggc atggcgctgg gtcgatccga
     4501 tccgcgaagc ctgggcccgg tccgatgaaa ctcccagggg atatcccgcc ggcagctggg
     4561 gaccggctgc ttccagttca ctgattgcgc gagacggtta tgcatggaac ggtgagtttt
     4621 agcccgcagg agcgcaggtt tccccatgtc gaggcgctca acgaggcgct cgcagccgac
     4681 atggcgtccg cgctcgagga aggactcggt aagggtcgtg gcgccagtct ggtggtggcc
     4741 ggcggccgca cgccggcacc ggcgttcgag gcgctgtcga acatcgaact ggattgggaa
     4801 gatgtctggg tgacattgac cgacgagcgc tgggtcgaca cccatatgga gggcagcaac
     4861 gagcacctgc tgcatcagca tctgctgcgc aaggaggcgg cgaaggccaa cctcgtcggt
     4921 ctcaagaaca aattccccga tcccgcttcg ggtgcggcgg cggcctggtc tgccgtgtcc
     4981 gagatcccgc ggccgtttga tttcgtgttg ctcggcatgg gcgatgacgg tcacatggcg
     5041 tcactgtttc cgaattcacc cggacttgaa aagggccttg atctgcaaca gcctccgggt
     5101 tgtatcgcgg cggtaggtcc tgccgagcct cgcgcccggc tcagcctgaa tctgcgtgcg
     5161 ctggtggatg cgcgacggat cgcggttctc atcgtgggcg cgtcgaaatg gggaacctat
     5221 cagcgagcgc gagcgccggg tccggtcact gaaatgccgg tcagggcgct gagcctgcaa
     5281 cagaacgttc cggtgacggt gtactggtcg ccttaggcga cgccgagatt gcgcagtccg
     5341 agactggcgt aacgtgaggc gcgcgagctg tagaccggtg ccggattcac gccgcgcagc
     5401 tgacccagta gcttctgtac ccggttgagc ttggcggcga cgatggcgcc gttcttgtcg
     5461 ttgatcgcct tgcaacgacg cgcgagttcc aggttcacgc tccagcgcgc atgcagcgcc
     5521 ttgctggggt cggcccactc gaataattgg tcgagcccgg tggcatccgc gccaaagcag
     5581 agcatgcggc cggtgtccgt gcgctccaca ccgagtcggc tcagcgcgtc gacgcatcgg
     5641 tggcggttgc tgccgatgtt ctgaatcgct tgcgcgtcgt caccgaccag gatgtcggtt
     5701 tcccgttcga gcaccgactc cagttcagcg agcaattgcg cttcctcggc caatatccgg
     5761 ctgaggtgct gccgcacatc atcacgcgag acgctcattt ttgcttggcg ctacccagat
     5821 cctgttcgag cgccagcatg ttgttggcaa tgcgctccgg attgattcga tagacgccgg
     5881 tcgcgagcgc ctgctgcacc gcagaaacgc gattcatgtc cacgtccggg ctgtcattca
     5941 ccgcctgcga gagtgaggcc agcgtccggg ccgagtcggt aatatgtacg ctgtccgagc
     6001 gcggagtgct cgcgggggcg ccagagcttc ccgagttaac aggggtagtg atgcgtatct
     6061 tttcaatggg attgctgctg tcgggtccga tcgacccgcc atccaatccc ttgatacggt
     6121 tagccacaac aagtctcctt agcgtcggtt cgagatctct atcggcctac gcgcgtcaaa
     6181 ctttagccgt tttccgtttg tcataacggg atctcgactt catttgagga ccggacgatc
     6241 ccttcgacca cgcgctgcga ggactgattc tggactttga tgcgctcgga ctcgtgcccg
     6301 tccgacaggg cgacgccgac catgcgcact tcaaagccac ccgcatgtgc cagcaaagtg
     6361 acctgctgac cgcgatgaat caggttcgct ggcgccagcg tctcgagcgt cagcgcatcg
     6421 ccggcggcca tgggatggcc caggcgttgg ccggcgaggg cagacgggtc gcagacgtag
     6481 gcgcttgcca gtcccggtac cagccgatcc tcaatccgaa aatccgccgc ggtcgcttcg
     6541 gcatcgcgcg tcagtgctct gcgggcgatc agcaccttcg cctgcgattg cacgttgacg
     6601 ctggtgtaga tcgtccagcg gatggatccc tcacaacgaa cgccaaccgt cgtctggctg
     6661 cgcacgctgc tgttgccggt catgaatgcg gtcaattgcc gatcacacga ggcgatacgc
     6721 aaccttgaat ccagtggcgc gacttgcagc gtcaggacac cggcggcagt gcctgcttcc
     6781 gagcgcaccg cgcgctccgc agccgcgcgg atcagtgccg gatcctcggt attcatagcg
     6841 ccggcggtgc tcgcggcagc aaacagcccg gcgtacaaga tgattctgag tgtgctcatc
     6901 gtcgatgaat aagagcaatc tgtgtgccat cggctcaggc tgtcgattcc ttgacgctca
     6961 agctttgagc gcgccgcgcc gataaggcga atcgacttgc ctgacgccgc gcgcgaggct
     7021 gcgcgttgcc agtgtgcggc aagcccttgc cgcgctgatc gtggtgaacg gtcctgacga
     7081 tgcgcgcgct tcgaactgcg tcacactttt ctgcagacgc acccgccctc gatcgctgtg
     7141 gcataggcct tgctcctatc gccgcatgcg aaccactttg gacaactatc ttggaatcca
     7201 tgcgcaggcg ctcaccatcg aggccaagcg aaacgagctg ctggcggcca acctggcgaa
     7261 cgtcgacacg cccaactaca aggcgcgcga catcgacttc aaggcagcgc tgtccgcggc
     7321 ctccggcaac agtgctgcca atggttcatc cagtctgtcg ttgcagcaag gcaatgcgtc
     7381 gctgaaagca acctcggcga cgcctacgac gggcacggag aactccggcg acgcctcgct
     7441 gatgcgctac cgcgtaccga tggcgccttc attggatggc aatacggtcg acgaacagct
     7501 cgagcagtcg gcgttcgccg aaaacagcgt gcgctatcag gccaccctga cctttctgaa
     7561 caacgcgttg cgcggactga tgaccgcgat caccggttca tgagtcctca agacttaagg
     7621 agtcatcaca gctatgtcca gcttcaagat attcgagatc gccggctcgg gcatgagcgc
     7681 gcagtcggtg aggctgaata cggtcgcgag caatcttgcc aacgctgaca gcgtcagcgg
     7741 cgatccgaac acggtgtatc aggcgaagca tccggtgttt caggcggtga tgggcagtgg
     7801 ggctgggctc aatgccgcca gcgccagtgt cagtgttacc ggcatcgccc agagccgcgc
     7861 tgcgccggaa atgcgctacg accctggcaa cccgctcgca gacgcgcagg gcaacgtcta
     7921 tgcgccgaac gtcaatgtgg tcgaagagat ggcggacatg atttccgcgt cccgctccta
     7981 tcagaacaac gtcgaagttc tgaataccac gcgcgacatg atgttgcaga cactcaagct
     8041 cggatcgtga tcaaccccta agaggaacca gccatgacta gcccagtcgg ctccccgacg
     8101 atggtcaatc ccagcggcac cgctggcagc agcggcagct cgagcgcctc gggcgccagc
     8161 tccagcatcg gcggtttgac gatgaacgat tttctgacgc tgatgaccgc gcaactgcag
     8221 aaccaggacc cactgaatcc gaccgacagc aatcaattcc tgacacagct gtccgagctc
     8281 agtacggtcg aaggtatctc acagctgaat acttcgatga gcacgctgtc gaactcgatg
     8341 ctgtcctcgc aggcgctgac cgcggcctca ctggtcgggc aaggcgtgct ggcgccggca
     8401 acctcagcct tctatacgag cggcacagcc atgacaggtg cggtgcaggt gccgagcggg
     8461 gccaccaacg tggtcctcag catcaccaac gccggcggcg cgctggtcga tcagctaaac
     8521 gtgcctgcct cagcgggact gcagagcttt agctggaatg gcaccgatgc aaacggccaa
     8581 gcgctgccga gcggcaccta ctcgatttcg gccgcggcac aggtcggcgg ttcgagccag
     8641 gctgccagca ctcttatcaa tggcaccgtg accagcgtga cgctgggagc cactggcagc
     8701 agtccgaccc tgaacaccct acagctggga cccgtgcccc tgagctcggt ccagcaaatc
     8761 gattgattct ttaaggagta cacctcatgc ccttcggtat cgcgctcagt ggcctggatg
     8821 cggctcaaag cgacctgaac gtcaccgcca acaacattgc caattcgcag acgaccggct
     8881 tcaagtcctc gaatgcgaac ttctcggaac tgtttgccaa ttccagctcg gcttcgagta
     8941 cgcagatcgg caacggtgtg cagctgcagg acgtcgagca ggagtttacg cagggcgata
     9001 tcaacaccac gggcaacagc ctcgacctgg cgctcagcgg caatggcttc ttcgccgtgt
     9061 cgcagggtgg cgccacgcag ttcacgcgtg ccggttcgtt ccacaccgac aatgccggca
     9121 atgtcgtcaa cgctgcaaac cagaacctgc aggtgtattc acc
//
LOCUS       DATMRM010000216         6962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_455718, whole genome shotgun sequence.
ACCESSION   DATMRM010000216 DATMRM010000000
VERSION     DATMRM010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455718"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VNZ06_06285"
     CDS             complement(<1..681)
                     /locus_tag="VNZ06_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HWW20394.1"
                     /translation="MSNKNFELKNPAGTSTALPVRSGTLGPDVLDIATINRDQGVFTF
                     DPAFGMTASCESKITYIDGDAGVLLYRGYPVEQLAERSSFMEVCYLLLYGELPSTQQL
                     DEFTDHIRRHTMVHESLLRFYNGFHHNAHPMAMVSAVVASMSAFYHDSTDIYNPRDRE
                     IFSHRIIAKLPTIAAAAHKHSVGQPFIYPRNDLDYCSNMLNMFFGVPCESYDINPVAS
                     KALDLLFIL"
     gene            complement(804..1019)
                     /gene="rpmE"
                     /locus_tag="VNZ06_06290"
     CDS             complement(804..1019)
                     /gene="rpmE"
                     /locus_tag="VNZ06_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HWW20395.1"
                     /translation="MKDAIHPDYKEITVTCSCGNTFKTRSTVGHDLSLEVCSNCHPFY
                     TGKQKIVDTAGRVDKFRKKYAGIGAAR"
     gene            complement(1411..2625)
                     /gene="hemW"
                     /locus_tag="VNZ06_06295"
     CDS             complement(1411..2625)
                     /gene="hemW"
                     /locus_tag="VNZ06_06295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HWW20396.1"
                     /translation="MTAALPPLALYIHFPWCVRKCPYCDFNSHTLHGELPESRYIEAL
                     LRDLTLQADRLQARPVHTVFLGGGTPSLFSPQAVSRLLAGVRARCTLVADAEVTLEAN
                     PGTIERGQFAGYRTAGVNRVSLGAQSFSNEQLQRLGRIHAATDTRDAVAELRDAGITN
                     FNLDLMYGLPHQSVSDALTDLDRAIALQPPHLSHYQLTLEPGTVFAGRPPPGLPDNDL
                     AADIQLACQERLASAGYIQYEVSAYTLEHQRCRHNLNYWEFGDYLGLGAGAHGKCSVV
                     EGTRLRVERTVRPREPRRYLAELDLNGAIERREVCAAELPFEYLLNALRLTEGFERQA
                     FEARTGLPFETVVAPFLSAQGKGLVEHRGERWSATPRGLNFLNDLQETFLPKTVGMQA
                     IGMSESSGTPRY"
     gene            complement(2622..3197)
                     /gene="rdgB"
                     /locus_tag="VNZ06_06300"
     CDS             complement(2622..3197)
                     /gene="rdgB"
                     /locus_tag="VNZ06_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HWW20397.1"
                     /translation="MLATANRGKQREFDALLAPLGIELLLQSELGIDGIAETGTTFEA
                     NALLKARHAAQASALPALADDSGLEVDALGGRPGVWSARYAGAASSDEDNNALLLREL
                     RGVPAQRRTARYRCVLALVRDAHDATPVIACGSWEGRIAERPAGDGGFGYDPLFLPDG
                     QAGSAAQLSADAKNAVSHRGKALAALVAQLR"
     gene            complement(3203..3919)
                     /gene="rph"
                     /locus_tag="VNZ06_06305"
     CDS             complement(3203..3919)
                     /gene="rph"
                     /locus_tag="VNZ06_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HWW20398.1"
                     /translation="MRRHGRGADALRPVRFTRRYTSHAEGSVLVEFGDTRVLCTASVE
                     EGVPGFLRGKGQGWITAEYGMLPRATHTRSPREAARGKQSGRTQEIQRLIGRSLRAAI
                     DLEALGERTVTFDCDVLQADGGTRTASITGAYIALVDACEALLSRRLILANPLHGQVA
                     AVSVGIVRGVAMLDLDYSEDSEAETDMNVVMNNGGAFIEVQGTAEGHAFRRHELDALL
                     NLAVGGIGELCALQTQALQS"
     gene            complement(3942..4685)
                     /locus_tag="VNZ06_06310"
     CDS             complement(3942..4685)
                     /locus_tag="VNZ06_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HWW20399.1"
                     /translation="MRVEYAELSLKGGRETNQDRICVAVAEDAALMIACDGMGGHAEG
                     ERAAEIAQRTVVERFWHTPQPLLDPLGFLHLSLGAAHVGVVTLGLPLPMELRPRATCA
                     LVLVQQGSAFWAHVGDSRVYHVRGGRVRERTRDHSHVELLVREGLINPGQVQNHPLRN
                     FVESCLGGEPMLPEMAMSPRRSLQPGDLLMVCTDGFWANLDETQIGSAFSAADKPLRD
                     TLSELSAQALLNAGALSDNTSVAALRFLD"
     gene            4755..5612
                     /locus_tag="VNZ06_06315"
     CDS             4755..5612
                     /locus_tag="VNZ06_06315"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HWW20400.1"
                     /translation="MTGFARNEFTGPFGTLVCEIRSVNHRFLDATLRLPDSARALEPE
                     LRQLLSRELKRGKVDCTVQQRGSEAGACNLEIDRGVLQRLLALVAELSKVLPEPAARV
                     DLIELLRFPGILRQDSADSEQLLQAARKLFADTVAELVRTRGREGERLASLIRQRCET
                     LGSMVSQVRARLPEVQARIRARFAERLKELGANVDQDRFEQEVLLMLQRFDVAEELDR
                     LDGHIQETAHTLAGNEPAGRRLDFLMQEFNREANTLSSKSQDLETTRSAVEMKVLIEQ
                     MREQVQNIE"
     gene            complement(6496..6801)
                     /locus_tag="VNZ06_06320"
     CDS             complement(6496..6801)
                     /locus_tag="VNZ06_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20401.1"
                     /translation="MKRLSREKLGLLGSWMVEEFSLFSLFPDEENLTDNFALTDAETV
                     GYGLGKLGLYTLLKRGGYVVAAYTLEVSAEVALAPLTIEATLINADLSEQASHCGCH"
BASE COUNT         1425 a         2195 c         2052 g         1290 t
ORIGIN      
        1 gaggatgaac agcaggtcga gcgccttgga agcaactgga ttgatgtcgt agctctcgca
       61 cggcaccccg aagaacatgt tgagcatgtt cgagcagtaa tccagatcgt tgcgcggata
      121 gatgaacggt tgaccgaccg agtgcttgtg cgccgctgcg gcgatggttg gcagcttggc
      181 aatgatccgg tgcgagaaaa tctcgcgatc gcgcgggtta tagatgtcgg tggagtcgtg
      241 atagaaggcc gacatcgatg ccaccacggc cgacaccatc gccatcgggt gcgcgttgtg
      301 atgaaagccg ttgtagaagc gcagcagcga ttcatgcacc atcgtgtgac ggcgaatgtg
      361 atcggtgaat tcatcgagct gctgcgtgct tggcagctcg ccgtacagca gcaggtagca
      421 cacctccatg aatgagctgc gctcggcaag ctgttccacc ggatacccgc ggtacagcag
      481 cacacccgca tcgccgtcga tataggtgat cttgctctcg catgaagcgg tcatgccgaa
      541 agccggatcg aacgtgaaca caccctggtc gcgattgatc gtggcgatgt cgagcacgtc
      601 gggcccgagc gtgccgctgc gaaccggaag cgcggtgctg gtaccggcag gattctttag
      661 ttcaaagttc ttgttgctca tccggcgcgg tccttgtgaa gcgagcgact ctactgcgcg
      721 atagtcagtc gtgcagactt gcatggacca cgagcggaat caaggcgcgc acccgtggcg
      781 cgccggtcag ctcactcaag cgctcagcgc gctgcgccga tgccggcgta cttcttacgg
      841 aacttatcga cgcggccggc cgtatccacg atcttctgct tgccggtata aaacggatgg
      901 cagttcgagc aaacctcgag gctcagatcg tggccgacgg tcgagcgggt cttgaacgta
      961 ttaccgcagc tgcaggtaac cgtgatctct ttgtagtcgg gatgaatcgc gtctttcatg
     1021 atgtgctctt ccgctatcac gcgtcggtcg cgttgaaccg cccgcgctgg aggcgggcga
     1081 aaaaaggcgc gcagtctagc cccgtcccag cagccctaca agtggattct tctacccacc
     1141 ggtcaagcgc caccgtagtg agccgaaagc gagtccgatt atccacagtt tctgtggata
     1201 actctgtgtg taagggcgac ggcattgcat tgcaagggca ttaaaagtgc gaatttgtca
     1261 aattgttcaa aaaatgatca atatgtttca gcctataaat tcaaacagtt atcgtactat
     1321 tatcgcgctg tgggaatgaa atgagacgta atcgactaat ttgtagaaac cccatcctcg
     1381 cgctgtgcat aaacccgttg tggccttcaa ctaataccgg ggcgtgccag atgattccga
     1441 catgccaatg gcctgcattc ctaccgtttt cggtaggaaa gtctcctgta agtcattgag
     1501 gaaattaagc ccccgagggg ttgcagacca gcgctcaccg cggtgttcga ccagcccttt
     1561 gccctgcgcc gaaagaaacg gcgcgacaac ggtttcgaag ggcaatccgg tgcgcgcctc
     1621 gaacgcctgg cgctcgaacc cttcagtcag acgaagtgcg ttcagcaggt actcgaacgg
     1681 caactcggcg gcgcacactt cacgacgctc gatggcgccg ttcagatcca actcggccag
     1741 atagcgtcgt ggctcacgcg gccgcaccgt gcgctcgacg cgcagccggg tgccttccac
     1801 cacactgcat ttaccgtgcg caccggcacc caatcccagg tagtcaccaa actcccaata
     1861 gttcagattg tgccggcagc gttgatgctc gagggtatac gccgatacct cgtactggat
     1921 gtacccggcg ctcgccagcc gctcctgaca ggcaagctgg atatctgcgg ccagatcgtt
     1981 gtcggggagg cccggtggcg ggcgtccggc aaacacggtg cccggctcga gcgtgagctg
     2041 gtaatgcgac agatgcggcg gctgcaacgc gatcgcccga tccagatccg tcagcgcatc
     2101 gctgacgctc tgatgcggca atccatacat caggtcgaga ttgaagttgg tgattcctgc
     2161 atcgcgcagt tcggcgaccg cgtcgcgcgt atccgttgcc gcgtgaatgc gaccgaggcg
     2221 ctgcagctgc tcgttgctga agctctgcgc tccgagcgag acgcgattga cgccggcagt
     2281 gcggtagccg gcgaactgtc cgcgctcgat cgtgccggga ttggcttcaa gcgtgacctc
     2341 ggcatccgcg acgagtgtgc atcgcgcgcg cactcccgcc agcaaccgcg acaccgcttg
     2401 cggcgagaac agactcggcg tgccgcctcc caggaacacc gtgtgcaccg gacgagcctg
     2461 cagccgatcg gcctgcagcg tcagatcccg cagcaatgcc tcaatgtaac gactctccgg
     2521 cagctcgccg tgcagcgtgt gcgagttgaa atcgcagtag ggacatttgc gcacgcacca
     2581 tgggaaatgg atgtacaggg cgagcggcgg cagagcggca gtcatcgcag ctgtgcgacc
     2641 agtgcggcca gggccttgcc ccgatgactg acggcattct tggcatcggc tgacagctgc
     2701 gcggcgctgc ctgcctggcc gtcgggcaga aacagcgggt cgtagccgaa gccgccgtcg
     2761 cctgcgggtc gctcggcaat ccgaccttcc cagctgccgc aagcaatcac cggagtcgca
     2821 tcatgggcat cgcgcacgag cgccaggacg cagcggtagc gtgcggtgcg tcgctgcgcg
     2881 ggcacgcctc gtagctcgcg caacagcaac gcgttgttat cctcatcgct cgaggctgcg
     2941 cccgcgtagc gcgcggacca gacacccggt ctgcctccca gcgcatccac ttccagtccg
     3001 gaatcatcgg ccagggcggg cagggcgctt gcctgcgcag catgccgcgc tttgagcagg
     3061 gcgttggctt caaacgtggt gccggtctcg gcaatgccat cgatcccgag ctcggactgc
     3121 agcagcagct cgattccaag cggcgccagc agcgcatcga attcgcgttg cttgccacga
     3181 ttcgcggtgg cgagcaccag tctcaagact gcagggcctg cgtctgaagc gcacacagct
     3241 cgccgatacc gcccaccgcg agattcagca gcgcatccag ctcgtgacgg cgaaacgcat
     3301 gaccctcggc cgtcccctga acctcaatga acgcgccgcc gttgttcatg accacattca
     3361 tgtcagtctc cgcctcggag tcctcgctgt agtccagatc cagcatcgcc actccacgca
     3421 cgattcccac cgaaaccgcc gccacctgtc cgtgcagcgg attggcgagg atcaaccggc
     3481 gcgatagcaa cgcctcgcag gcatccacca gcgcgatgta ggctccggtg atcgaagccg
     3541 tgcgcgtgcc cccgtcagcc tgcagcacgt cgcaatcgaa cgtcacggta cgctcgccga
     3601 gggcttccag atcgatcgcc gcgcgcagcg agcgaccgat cagacgctgg atctcctggg
     3661 tgcgcccgct ctgcttgcca cgagccgcct cgcgcgggct gcgggtgtgc gtggcccgcg
     3721 gcaacatgcc gtattccgcg gtgatccagc cctgtccttt gccgcgcaga aagccgggca
     3781 cgccttcctc gacactggcg gtgcacagca cccgcgtgtc gccgaattca accagcaccg
     3841 agccttccgc gtgcgaggta tagcggcgcg taaaacgtac cggccgtagg gcatcggcac
     3901 cgcggccgtg tcggcgcact gaggtattca gggaaattct cctagtcgag gaaacgcaac
     3961 gccgccaccg aagtattgtc gctgagcgcg ccggcgttga gcagcgcctg tgccgacagc
     4021 tccgaaagcg tatcccgcaa cggcttgtcg gccgccgaga aagcactgcc gatctgggtc
     4081 tcgtccaggt tggcccaaaa accgtcggtg cataccatca acaggtcacc gggctgcagc
     4141 gagcgtcgcg gactcatcgc catctcgggc agcatcggct cacctcccag gcatgactcg
     4201 acgaaattgc gcagcgggtg gttctgcacc tggccgggat tgatgagccc ctcgcgcacc
     4261 agcagctcga cgtgactgtg atcgcgggtg cgttcgcgca cgcgtccacc gcgtacgtga
     4321 tagacccggc tgtcacccac gtgcgcccaa aaggccgagc cctgttgaac caggacgagc
     4381 gcacaagtgg cccgcggccg cagctccatc ggcaatggca aacccagcgt cacgacaccg
     4441 acgtgcgcgg cacccagtga cagatgtaga aaccccaacg gatccagcaa tggctgtggc
     4501 gtatgccaga agcgctcaac caccgtgcgc tgtgctatct ctgcggcgcg ctcgccctcg
     4561 gcgtgaccgc ccatgccgtc gcaggcaatc atcagggcag cgtcttcggc taccgccaca
     4621 cagatgcgat cctgattggt ctcgcgcccg cccttcaggc ttaattctgc atattcgacc
     4681 cgcaagcgag ctccggcgct ttgcgctaga cttcgcgcgc tttatacacc atttgactcc
     4741 ccatgatcgc aagcatgacg ggttttgccc ggaacgagtt caccgggccg tttggcaccc
     4801 tcgtgtgtga aattcgcagt gtcaatcacc gatttctgga cgcgacgctg cgtctaccag
     4861 acagcgctcg cgcgcttgag ccggagctgc gccagttatt gtcacgtgag ctcaagcgcg
     4921 gcaaagtgga ttgcacggtg cagcagcgcg gctcagaggc aggggcttgc aatctggaga
     4981 ttgaccgggg ggtccttcaa cggctgctgg cgctcgtggc ggagctatcg aaggtgctac
     5041 ccgagcccgc ggcccgagtg gacctcatcg agctgctgcg attcccggga atacttcgcc
     5101 aggacagtgc cgattcggaa cagttgctac aggcggcacg caaactgttc gcggataccg
     5161 tcgccgaatt ggtacgaacg cgcggccgtg aaggcgagcg gcttgcaagc ctgatccgac
     5221 aacgctgcga gacgctggga tcgatggtgt cgcaggtgcg cgcgcgcctc ccggaggtgc
     5281 aggcgcgtat tcgggcacgc tttgccgagc gactgaagga actcggtgct aacgtggacc
     5341 aggaccgatt cgaacaggaa gtgctattga tgctgcagcg cttcgatgtc gcggaggaac
     5401 tggaccgtct tgacggccat atccaggaaa cggcacacac gctcgcgggc aacgagccgg
     5461 cagggcgccg gctcgacttc ctgatgcagg agttcaaccg cgaggccaac acgttgtcct
     5521 cgaagtcgca ggatctggaa accactcgat cggccgtcga gatgaaagtg ttgatcgagc
     5581 agatgcgcga acaggtacag aatatagaat agttacttaa gttattgaat aataagaatc
     5641 tatatctcag caatctttcc acactatcga cccttgcccc tcaaccgtat gcagcgccgc
     5701 ctgcagcaga tcgccgcctc gatcccaagg cccaacgcgc tgcgctcgtt tccccagcgt
     5761 atgtcgttga cgcagcagcg caaagcagct aaacgaggcc gactctggca gccatcaaag
     5821 atgcgtcggg tgtgccctct atcattcgcc accacaaccc ccgcaccacc gccgaaactc
     5881 cggaccgcgg catctatccc cgacaccgac gcgaccatac cggccacgga ctgcctttag
     5941 ccctttagcc gccgccgctc tccaagccaa gccgcgaccg accgcgatgc gccagggttc
     6001 gccggtgccc agacgcttac tgatccggca tatgcataga cccggtgtca tttcacgtcg
     6061 gcgttatttc tgctcataga taacaatatg agtgctctca atccgcagag agcgttagct
     6121 acgctccacg gaagaagtcc aattgccacg atgcccgtaa atgcgagtcc gccgtggcgg
     6181 atcgacacag acaccgggag atgcgggtgc actaggagtc caaccatcgc gtctatcgca
     6241 accagtgcgc ccaagactga cagtccctgc accgcggctt taaaccagtc acgggcagcg
     6301 cgtcgtctcc cggcctggct ctgcagcaat tggtaaacaa atagcaaaaa tatggccaca
     6361 gcgccaagcg cgatcatgac attccgcaat tgttgctcag gtccagacag cgcaaacgcg
     6421 cctagcacgc caaccgcaag cgggactagc atgcaggctg tggctacagc ctgaccagcc
     6481 aaagtaggtt ctctattagt gacaaccaca atgactggcc tgctctgaga gatctgcatt
     6541 gatgagcgtc gcttcaatcg tgagcggagc gagcgccacc tcggcactca cttccaaggt
     6601 ataggccgct actacgtaac cacctcgctt gagaagcgtg taaaggccga gcttcccgag
     6661 cccatatcca accgtttctg catcagttag agcaaagtta tcagtcaagt tttcctcatc
     6721 cgggaacaag ctgaaaagac tgaattcctc aaccatccat gaacccaata gtcccagttt
     6781 ctctcgcgag agtcgcttca tgcattgtcg atcaacagga agtcggacaa tctgagggcc
     6841 aggtcctacg ccagtgcctt tcccgctctg cccgagcggg tcaaagttgt cgatcggatc
     6901 gccgtcggca tatgcgtaaa ttcaggtctg agcagggggt cggaaatgct gtcgaaacac
     6961 ga
//
LOCUS       DATMRM010000217        13498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2606298, whole genome shotgun sequence.
ACCESSION   DATMRM010000217 DATMRM010000000
VERSION     DATMRM010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13498
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2606298"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VNZ06_06325"
     CDS             complement(<1..962)
                     /locus_tag="VNZ06_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWW20402.1"
                     /translation="MNTRTWMMGAVAATVALLSARPALSAAGDQIVIGDIDDFSGLYA
                     DEGGNGGVESIKMAIADFGGTVLGKKISVLSADHQNKPDIGSSKFREWADQDGLNMML
                     GGSNTGVNIAMAKVAGAKKIPYFAIGPGGASLTNEDCTPYTVHYVYDTTALANGTATA
                     IVQGGGKTWFYLTADYAFGTQLQAAASKVVEANGGKNIGAVRVPLSASDFSSFLLQAQ
                     SSGAQVLGLANAGEDLTNSLKAANEFGITRKMKPAALLAFINNIHSLGLQTAQGLYLT
                     TAFYWDLNDETRAFSKRFFDKFKREPSMDQGGFYSATLTYLKAV"
     gene            complement(992..1696)
                     /locus_tag="VNZ06_06330"
     CDS             complement(992..1696)
                     /locus_tag="VNZ06_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWW20403.1"
                     /translation="MSAELLRVAGLHAYYGESHILHGVDLTVSEGEVVTLLGRNGAGR
                     TTTLRALLGLISKRSGSVMIHGQEAIGLPAHKIAHLGVGYCPEERGIYASLSAEENLV
                     LPPIVRTGGMSLAEIYTMFPNLQERRSTQGTRLSGGEQQMLAVARTLRTGARLLLLDE
                     ISEGLAPVIVQGLARTIQELRARGYTIVMVEQNFRFAAPLADRFYIMEHGRIVEAFAA
                     SELQSRMSTVHRFLGV"
     gene            complement(1693..2445)
                     /locus_tag="VNZ06_06335"
     CDS             complement(1693..2445)
                     /locus_tag="VNZ06_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWW20404.1"
                     /translation="MADNLIIETRGLTKEFNGFFAVSEVDLRVRRGQIHALIGPNGAG
                     KTTCFNLLTKFLIPTRGHILYKGQDITLAKPAEVARLGIVRSFQVSAVFPHLTVLENV
                     RIALQRTLGTSFHFWKRESSLAVLNSRAMELLEEVDLAALAAKVAVELPYGQKRALEI
                     ATTLAMEPELMLLDEPTQGMGHEDVGKVTALIKRAAANRTVLMVEHNMNVVSTIADTI
                     TVLQRGRVIADGPYAEVSKDPLVVEAYMGSAA"
     gene            2772..3599
                     /locus_tag="VNZ06_06340"
     CDS             2772..3599
                     /locus_tag="VNZ06_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWW20405.1"
                     /translation="MKRSPRLTQREFRTALPRLFAAMKASDAIILRHFRNARIDHKPD
                     GSEVTIADKGAERILRQHLRSAWPRDQVLGEEYGGELTRSGRCWLVDPIDGTASYVLG
                     IPMFGTLICLLIDGEPVLGCIHLSALEETTYAASGFGCWLARPGVRARRVGVALPRPL
                     SQAQVGLSNIKHSDLLRQAGPLRLSGLLRNAGRLRLVGDCVQYALVCRGAIDAAVDPL
                     MNPWDIGPLVVCIREAGGAVSDLQGNTTRVVERRSFVAASSHRLRRAICREVGTGQS"
     gene            3617..4345
                     /locus_tag="VNZ06_06345"
     CDS             3617..4345
                     /locus_tag="VNZ06_06345"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWW20406.1"
                     /translation="MIEHHIDIATPDGQMNTFITHPEENGPFPVIFFYMDAPGKREEL
                     HDMARRIATVGYYVVLPNLYYRVTREFTMVRDEEGFKKMFDLMRQTTVANVMRDTRAM
                     LDYVDGQSAAEASSLGGVGYCMSGPFILAACAQFPDRFSCGASIYGAGLVTKSGDSPH
                     LMADRVRGEMYFACAETDKYAPKEVIDEMLSHFRTTRLNYRLEWYPGAEHGFAFPLRA
                     GIFHKPSAERHWSRLFALFQRNLR"
     gene            complement(4472..6112)
                     /locus_tag="VNZ06_06350"
     CDS             complement(4472..6112)
                     /locus_tag="VNZ06_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWW20407.1"
                     /translation="MKMTGSRAVSVGAFLAVLPMFVNAGADTAERLGTVSFQTSCAPA
                     VSADFNRGVALLHDFWYEEARPQFERILKADPSCAMAHWGIALSGFHQIWERPGAAVM
                     AKGWHEMQAAEATPAKTQRERAYLAALSDFFRPGADDYSKRIERYATALGKLYADNPQ
                     DVDAGAFYALALIAADSDESGVAAERKAMAVLTPLWKKYPDHPGLVHYIIHACDNPAM
                     ATDGLAAANQYGEIAPSGPHAVHMPGHIYARLGMWRQDIAVNRASVQASEAAEAHHES
                     GAMDQFHSDDFLLYAYLQSGQDANAKATIAASSAAIAHFESMPDMVSSHYMVGMFPNY
                     RVKLPIFYALEMRDWKAATELQPIKGAPADTQAQVYWARTIGDGHLHQGKQAQSDLAA
                     FDALLEEVKKGSFAYLASGMGSRIRHQEMLAWVAFAQGNTEEAVKQMSAAADLQDKVG
                     QGEVDIPAREMLADILLESGRANEALANYQQALNLSPNRFNGLYGAGKAAEMVGNTAV
                     ARNFYVALLKSTDNGTMTARTEVKHAQTFISSASAGTH"
     gene            complement(6269..9115)
                     /locus_tag="VNZ06_06355"
     CDS             complement(6269..9115)
                     /locus_tag="VNZ06_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWW20408.1"
                     /translation="MKTSKAVAYAVSLAIAAAAAPGMAADNATDASAELQEIVVTGTS
                     IKGVTETSLPVQILSSEDVQRTGATNVEQLFRQIAAASSVGSTVASQATGFTTGAIST
                     ISLRGLGSERTLVLINGLRSAAYGGSAVGGASTSVDISSIPVSAIERVEILKDGASAI
                     YGSDAIAGVVNFILKSDFQGLSISANGGTPTHAGGGSEEGASLFAGIGDIKSDGYNVN
                     FGINYDHQGQILGNHRSFAGRYQPQYGNDVTSSFAFPANVNVPGSHAAFTNPAFPKGS
                     TQNPDAGNCGPYSLNDVNFPAQCRFDNTPFDDLQPDITKLGFLLQAELALNDSNKLYG
                     LASFSQVRTITEVQPVPLSNGNPLLPSDPYYNYLATALAPGGQFAGYGAINPGVKAGT
                     GAFLLPNTSPYYPLAAAEKYGLCTGATASTCQPLNLIYRDFANGVRVTEDLANTTRLI
                     GGIKGDALGWDYDAHLLFSSVGVYEDLDHGFALYSKVMPLLDQGNINPFGPTTDASQI
                     ALAQQDAFYGQDFRSRTSLASGDFTVSRDLFQTPWGPFAGALGGEIRRETFSYQPSAA
                     IQTGDIAGEGGNALPVFATRSAESAYVEFNSPILPALTADVAVRWDNYQRVGDTVNPK
                     GSLKWEPTAWLLLRGSAGTGFRAPSLTDLYAPQTRSVTSNGTRDPIQCPVFSAANPAC
                     SFQFTTIQGGNPNLQPEKSNQYSLGTVLKPVQNLTLDFDAWWIFLRNSIVGGGLPYQT
                     ILANAASAAQFSNLIIRDAAGNIVSISQQNSNLFKSAVSGLDMDFRYFVDLPSQGRVT
                     ADVNGTYFLKYQTELVTNNGDYTNFIDQPYTITADSGVISRFRYNATLDYAVGDFEVS
                     LIEHYQKRYHDAPSSISGAKRFVGQYQTFDLQGSYTLAKSTQFTLGIINLFNTAPPYA
                     NYAASANNFVGGYDLSYGDARGEFVYARVTYQFH"
     gene            complement(9480..11372)
                     /locus_tag="VNZ06_06360"
     CDS             complement(9480..11372)
                     /locus_tag="VNZ06_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWW20409.1"
                     /translation="MLSWFDSTEATGVGVALAQDVASTVTIRMRRADQSAAQQDPKHL
                     RAFLKKIASQAGSLRLNLYKKAKLASSFKTKLLDNGIEPALAEHLMQLLLTQLASEQR
                     DAASSDPVEAVRHKRPVFRKTEELLAEAHKFARQGRHREAADAFLQIVQQEPGNADGW
                     HGFGAAQWKLGAYDEAENYFRRVLELQPSNAEALCNLGAALYSRGEYPEAETVLRRAI
                     KSKSTFLRAQCNLANVLIMQARVSEAEALLTKVLRLAPRDAEALLCMGRIERLNGRFD
                     DARQLFIRALESRPRMPAAWAELAGLRRMTAADSEWQRGAEEILASAIEPQEEAELRF
                     ALGKYFDDLGDYERAFKHYQRANQLRRKVAPKYDPYAREAAVRDLIGGYSPNPSRVST
                     PLECAKPVFVVGMMRSGTSLVEQIIASHPRATGAGELTFWNDAYRAYEAEIRSGRLGE
                     SVRRQLGERYLRLLRTHGADSKVVVDKANVNADYLGLIHTIFPGARFICVTRNPIDTC
                     LSCYFQPLSPSYGYKFDLAELAHYSRQHELLMRHWKSVLPSAAILEVPYEQLVSHKEQ
                     WIRKMLDFVGLDWQPQCLNFSQTSRAVKTASAWQVRQSMYHSSVGRWRHYEKYLGPLR
                     GLTRQS"
     gene            11708..12535
                     /locus_tag="VNZ06_06365"
     CDS             11708..12535
                     /locus_tag="VNZ06_06365"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HWW20410.1"
                     /translation="MCLVLGATGLRASLAQAQTPSPLQEWQYSGGVILARLFEPALPT
                     FRTITGLAAELQPAYDGSRAYRLEGGPDVNVYYRDVAFISTGDGIGYNVLRGSHYQVG
                     VAMTYDLGRRVDQDDANLHGMGNISPAPVAKLYASWVLSKKLPLILRVSARQFIGGAQ
                     GAVGDIAIYTPLPGSSHTFVMFAGPSLTLATNHYLQVLYGVTPLQSRASGHPIYAIAH
                     DGTAAAGVGFSATKFFGSHWLINFDGALSQIRGKPARSPVVEKINQHVAVLSLNYQW"
     gene            complement(12557..>13498)
                     /locus_tag="VNZ06_06370"
     CDS             complement(12557..>13498)
                     /locus_tag="VNZ06_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWW20411.1"
                     /translation="KPKNTAASSAQSAAATAPVTAPDGPTDVAASTVPNPKEEASPTA
                     VASDADDPADYARRGEAFESRRDFDQALTALNRACDLAPDNAEYVYRRGLLHEELKQL
                     APAMADFDRSLQLRPNDLNTLLARAELKIRTGDKPGGRMDLDAADAIAPKQANERFRM
                     ALAYEHADQFDPAISQMNLWIDSHGEDARLPNVLNERCWIRGLGGMDFPLALKDCNSA
                     MRLTDKSSSLYARIADSRGLILLRMGDFNKSIADYDTAVKLNPKDAWAWYGRGIDKLR
                     SGRAQQGNADIAQAVTLWPRVAEEYKRLGIISDVPTP"
BASE COUNT         2628 a         4164 c         3949 g         2757 t
ORIGIN      
        1 ttcacggctt tcagataagt cagcgttgcc gagtagaaac cgccctggtc catagacggt
       61 tcgcgtttga atttgtcgaa gaaccgcttg ctgaaggcac gagtctcgtc attgaggtcc
      121 caatagaagg cggtcgtcag atacagcccc tgcgcggtct gcagccccag gctgtggatg
      181 ttgttgatga aggccagcag cgcggcaggc ttcatctttc gggtgatgcc gaattcgttg
      241 gctgccttca gtgagttggt caaatcctcg cccgcgttgg ccaagccgag cacctgggcg
      301 ccggaactct gcgcctgcag caggaacgac gagaaatccg aggccgacag cggtacccgc
      361 accgcgccga tattcttgcc accatttgct tcgacgactt tagaggctgc cgcttgcagc
      421 tgcgtgccga aggcgtagtc ggcggtcaga tagaaccagg tcttgccccc gccctgaacg
      481 atggcggtcg ccgtaccatt ggccagcgcg gtcgtgtcgt agacgtagtg cacggtgtag
      541 ggcgtgcagt cttcgttggt caatgatgcg ccgcccggac cgatcgcgaa atacgggatc
      601 ttcttggcgc cggccacctt ggccatggca atgttgaccc cggtattgga gccgccgagc
      661 atcatattga gcccgtcctg gtcggcccac tcgcgaaatt tggacgagcc gatatcaggc
      721 ttgttctggt gatcggcact gagcacgctg atcttcttgc cgagcacagt gccgccaaaa
      781 tcggcgattg ccattttgat ggactcgacg ccgccgttgc cgccctcatc ggcatacaag
      841 ccagagaaat cgtcgatgtc gccaatgaca atctgatcac cggcggcact cagtgccggc
      901 cgcgcgctca gcagcgcgac ggtcgccgca accgcaccca tcatccaagt cctcgtgttc
      961 attggcatcc cccgcatgaa gaaatcgagc cctatacgcc cagaaaccga tgcaccgtgc
     1021 tcatcctgct ctgcagttcc gatgcagcaa aggcttccac gatccggccg tgctccataa
     1081 tataaaaacg gtctgccagg ggcgcagcaa aacgaaagtt ctgctccacc atcacgatcg
     1141 tgtagccgcg agcccgtagc tcctggatcg tgcgggccag cccctgcacg atcaccggcg
     1201 ccaacccttc cgaaatctcg tccagcagca gcagccgcgc gccggttcgc agtgtacggg
     1261 cgaccgccag catttgctgc tcaccaccgg aaagacgcgt gccctgcgtg ctgcgccgct
     1321 cctgtaggtt cggaaacatc gtgtagatct cagcgagcga catgccgccg gttcgaacga
     1381 tcggcggcag caccaggttt tcctcagcgg acaggctggc gtagatgcct cgctcttccg
     1441 ggcaatagcc aacacccaga tgcgcaatct tgtgcgccgg cagcccgatg gcctcctgcc
     1501 cgtgaatcat caccgatccg gaacgcttcg atatcagccc cagcagcgcc cgcagggttg
     1561 tggtccgtcc ggcaccattc cttcccagca acgtgacgac ttcgccttcg ctgactgtca
     1621 gatccacacc atgcaggata tgagactcac cgtagtacgc atgcaggccg gcgacgcgca
     1681 gcagctccgc gctcatgccg ccgaacccat gtaggcctcg acgaccagtg ggtctttcga
     1741 cacctctgca tacgggccgt ctgcgatgac gcggccgcgt tgtaacaccg tgatcgtgtc
     1801 ggcaatggtc gacacgacat tcatattgtg ttcaaccatc agcaccgtgc gattggcagc
     1861 cgcgcgcttg atcagtgccg tgaccttgcc gacatcctcg tgacccatgc cctgcgttgg
     1921 ctcatccagc aacatcagct ccggctccat cgccagcgtg gtggcaatct cgagcgctcg
     1981 cttttgtccg taaggcagct cgaccgccac tttggcggca agcgctgcga ggtcgacctc
     2041 ttccagcagt tccatcgcgc gcgaattgag cacggccaga ctcgactcgc gcttccagaa
     2101 atggaatgac gtacccagcg ttctttgcag cgcgatccga acattctcga gcacggtcag
     2161 gtgaggaaac acggccgaca cctgaaacga tcgcacgata cccagccgcg cgacctcggc
     2221 cggcttggcg agcgtgatgt cctgaccctt gtagagaata tggccgcggg tgggaatcag
     2281 aaacttcgtg agcagattga agcaggttgt cttgcccgcg ccattcggcc caatcaatgc
     2341 atgaatctgc ccgcggcgca cacgcaggtc cacttcgctc acggcgaaaa agccgttgaa
     2401 ctccttggtc agcccacgcg tttcgataat caaattgtca gccatatcct taggtagggc
     2461 tcggctgggt tggcggttgc gccgttcttt tggccgccat tatgcggcat gaagatgacc
     2521 atttggccag aactcgctat ctcaattatt aagcttaata atcattgttt tacaaaaata
     2581 acctcaagtt aattagaaca gctccctgag gcacaggcgc tggcgcctga tcttgcgccg
     2641 ctatcgactt ttctgttccc gtgaccgaag ctgggtgccg caatcttttc gcggttcccc
     2701 gcgacattga ccgaaacctt cggcaaacta cacgctacaa cgaactacgg atttcactac
     2761 cgattacccg cttgaagcgc tcaccgcgat tgacgcagcg cgaatttcgc acggcactgc
     2821 cgcgcctgtt cgcggccatg aaggccagcg acgccatcat tctgcgccac tttcgcaatg
     2881 ccaggattga tcacaagccc gacggcagcg aggtaacgat cgcggacaaa ggcgcggagc
     2941 gcatcctgcg tcagcatctt cgctccgcgt ggccgcgcga tcaggtgctc ggtgaggaat
     3001 atggtggcga actcacgcgc agcggacgtt gctggctggt ggatcccatc gatggcaccg
     3061 cgtcctacgt gctcggcatt ccgatgttcg gcaccctgat ttgcctgctg atcgacggcg
     3121 agccggtcct cggctgcatt catctgtcag cgctggagga gaccacctac gcggccagcg
     3181 gctttggctg ctggctcgct cgtccggggg tgcgagcgcg acgcgtgggc gtcgcattgc
     3241 cccgtcctct gtcgcaggcg caggtgggcc tcagcaacat taagcacagt gatctgctgc
     3301 gccaagccgg cccgttgcga ctgtcaggtc tgctgcgcaa cgccggacgc ctgcggctgg
     3361 tgggcgactg cgtgcagtac gcactggtct gtcgcggggc aatcgatgca gccgtcgatc
     3421 ccctgatgaa tccgtgggac attgggccac tcgttgtgtg catacgcgag gccggcggtg
     3481 ctgtcagcga cctgcaaggc aacaccaccc gagtggtcga acggcgctca ttcgtcgcgg
     3541 ccagctccca ccgcttgcgc cgcgcgatct gccgcgaggt cggtaccgga caatcatagt
     3601 caagggggat ggatccatga tcgaacacca tatcgacatc gcgacgccgg acgggcaaat
     3661 gaataccttc atcacccatc ccgaggagaa cggtccgttc ccggtgatct ttttctacat
     3721 ggacgcaccc ggcaagcgcg aagagctgca cgacatggcg cgacgaattg cgaccgtcgg
     3781 ctactacgtc gtgctgccga acctctacta ccgagtcaca cgcgaattca cgatggtgcg
     3841 cgacgaggag gggttcaaga aaatgttcga cctgatgcgc cagacaaccg tcgccaacgt
     3901 gatgcgcgac acccgagcca tgctcgacta cgttgacggg cagtccgccg ccgaagcgag
     3961 ttcgctcggt ggagtcgggt actgcatgag cggacccttt atcctggcag cttgcgcgca
     4021 atttcctgac cgtttcagct gcggcgcttc gatctacggt gccggactgg tcacaaaatc
     4081 cggcgattca ccgcacctga tggcggaccg tgtccgcggc gagatgtatt tcgcctgcgc
     4141 cgagaccgac aaatacgcgc ccaaagaagt gatcgatgaa atgctttcgc acttcaggac
     4201 cacgaggctc aactatcggc tggaatggta tcccggcgcg gaacatggct ttgcctttcc
     4261 gttgcgcgcc gggatcttcc ataaacccag tgccgagcgg cactggtcgc gcctatttgc
     4321 attgttccag cgcaatctga gatgagcgtt acagagcgcc gcgccctgag cggctcgggc
     4381 tcgcgccgcg cgatccgcag tcggcaccaa tgaacacggt gcgatgccga gccgcccagc
     4441 gtgccgggca gcgaagcggc aaccgctgcg gtcaatgcgt tcccgcgctc gccgaggaaa
     4501 taaacgtctg cgcgtgcttg acctcggtgc gcgcagtcat ggttccattg tcggtcgatt
     4561 tcagcagggc aacgtagaag ttacgcgcaa ccgcggtatt gccgaccatc tcggccgcct
     4621 tgcccgcacc gtagagccca ttgaagcgat tcgggctgag attcagtgcc tgctggtagt
     4681 ttgcgagagc ttcattggcc cgaccggatt ccagcagaat atccgccagc atctcgcgcg
     4741 ccgggatgtc cacttccccc tgtccaacct tatcctgcag atcggctgca gcgctcatct
     4801 gtttgaccgc ttcctcggtg ttgccttgag cgaatgctac ccaggccagc atctcctgat
     4861 gccgaatgcg cgagcccatg ccgctcgcta gataggcgaa cgaacccttc ttgacctcct
     4921 cgagcagcgc atcgaatgcg gccagatcgc tctgcgcctg tttcccctgg tgcagatgac
     4981 cgtccccgat cgttcgcgcc cagtacacct gtgcctgggt gtccgccggc gcacctttga
     5041 tcggctgcag ttcagtggcc gccttccagt cacgcatttc cagtgcatag aagatcggaa
     5101 gcttgacgcg ataattcgga aacataccaa ccatgtagtg cgacgagacc atgtccggca
     5161 tcgactcgaa gtgcgcgatc gcggcactgg aagcagcaat cgtcgccttg gcattggcat
     5221 cctgcccgct ttgcagatag gcgtagagca ggaagtcatc ggagtgaaac tgatccatcg
     5281 cgccgctttc atggtgtgct tccgcggcct cggacgcctg caccgatgcc cgattcaccg
     5341 caatgtcctg ccgccacatg ccgaggcgcg cgtagatgtg tcccggcata tgcaccgcgt
     5401 gcggtccgga tggggcaatc tcgccgtact gattcgccgc ggccaggccg tcggtagcca
     5461 tcgccggatt gtcgcaggca tggatgatgt aatgcaccaa gccgggatgg tccgggtatt
     5521 tcttccacag gggcgtcaac actgccatcg ctttgcgctc ggccgcgacg ccgctctcat
     5581 ccgagtccgc agcaatcagt gctagcgcgt agaaggcacc cgcatcaacg tcctgcggat
     5641 tgtcggcata gagttttccg agcgccgtcg cgtagcgttc gatgcgcttc gaatagtcat
     5701 cggcgccagg gcggaaaaaa tcactcagcg ccgcaaggta ggcacgctcg cgttgcgttt
     5761 tcgccggcgt cgcctccgcg gcctgcattt catgccagcc cttcgccatc acggcggcac
     5821 cgggccgttc ccagatctga tggaagccgc tcagcgcgat gccccaatgc gccatggcgc
     5881 aggaggggtc cgccttgagg atgcgttcga actgcggccg ggcttcctcg taccagaagt
     5941 catggagcag cgcgacgccg cgattgaaat cggcactgac cgctggcgcg cacgaggttt
     6001 gaaacgacac cgttcccaga cgctcggccg tatccgcacc ggcgttgaca aacattggca
     6061 gcacggcaag gaacgccccc acactgaccg cgcgcgagcc cgtcattttc atggcgggga
     6121 ttctagcagc tggcgccgaa cggacgacgg tggtcatcag tagctgcgct gcagcgcaat
     6181 actgctctca agagacggcg cggcggctgg ctggtgccag acggccgcgc aagttgagtg
     6241 acgttattgt ggttggaagc tgattgtttt aatggaactg gtaggtcaca cgggcataca
     6301 cgaactcgcc ccgtgcatcg ccatagctga ggtcatagcc accaacgaaa ttgttggcgc
     6361 tggccgcgta gttcgcatag ggcggagccg tattaaagag attgatgata cccagcgtga
     6421 actgggtgct cttagccagc gtgtacgatc cctgcagatc gaaggtctgg tactgaccga
     6481 caaagcgctt tgcaccactg atcgaggacg gcgcgtcgtg atagcgcttc tggtagtgct
     6541 ctatcagcga gacctcgaag tcgccgacgg cgtagtcgag cgtcgcgttg tagcggaagc
     6601 gtgaaatgac accactatcg gctgttatcg tatacggctg atcgataaag ttagtgtagt
     6661 cgccgttgtt ggtgaccagc tcggtctggt acttgaggaa gtacgtaccg ttgacgtccg
     6721 cggtaacgcg accctgcgaa ggcaggtcta cgaagtagcg gaagtccatg tccagcccgc
     6781 tgaccgcgga tttgaacagg ttcgagttct gctggctgat cgacacgata ttaccggcgg
     6841 cgtcgcgaat aatcaggttc gaaaattgag cggcagaggc cgcgttggcc agaatcgtct
     6901 gatacggcag accgccaccc acgatcgagt ttctcaggaa tatccaccag gcatcgaagt
     6961 cgagagtcag gttctgcaca ggcttaagga cggtgcccag tgagtactga ttcgactttt
     7021 caggctgcag gttggggttg ccgccctgaa tcgtcgtgaa ctgaaaactg cacgccgggt
     7081 tggcggctga gaaaacaggg cattgaatcg ggtcacgtgt accattcgag gtcacgctgc
     7141 gggtctgcgg cgcgtacagg tccgtcaatg aaggcgcacg gaatccggta cctgccgatc
     7201 ctcgcagcag cagccaagcg gtcggctccc atttcaacga tcccttcgga ttcaccgtat
     7261 cgccgacccg ttgatagttg tcccagcgta cagccacgtc tgcagtcagc gccggtagga
     7321 tcggagagtt gaattcgacg taggccgact cggcagaacg tgtcgcaaac accggcagcg
     7381 cgtttccacc ctcacctgca atgtctccgg tctggatcgc cgccgacggt tggtagctaa
     7441 acgtctcgcg acggatttcg ccacccagcg ctcccgcaaa ggggccccag ggtgtctgga
     7501 acaggtcacg gcttaccgtg aagtcgccac tcgccaggga agtgcgcgac cggaagtcct
     7561 ggccatagaa cgcgtcctgc tgcgccaatg caatctgact ggcatccgtg gtcggtccaa
     7621 acgggttgat attgccttgg tccagaagag gcatgacctt tgagtacagc gcgaagccgt
     7681 gatccaggtc ctcatagaca cccacggagc tgaacagcag atgagcgtca tagtcccaac
     7741 cgagcgcgtc gcccttgata ccaccgatca aacgggtcgt attcgccaga tcttcagtca
     7801 cccgcacgcc attcgcgaaa tctcggtaga tcaggttcaa aggctggcat gtgctcgcgg
     7861 tcgcgccggt gcacagtccg tacttctcgg ccgctgcaag cggatagtaa ggactggtgt
     7921 tcggtaacag gaatgcaccc gtgcccgcct tgacacccgg attgatggcg ccgtaacccg
     7981 caaattgccc tcccggcgcc aacgcggtcg ccaggtagtt gtaatacgga tccgaaggca
     8041 acagcggatt tccattcgac aggggtacgg gctgcacctc ggtaatggtc ctgacctgcg
     8101 agaagctcgc caggccgtac agcttgttgc tgtcgttcag cgccagctcc gcctgcagca
     8161 ggaagccgag cttggtgata tcgggctgca gatcgtcaaa gggtgtattg tcgaaccggc
     8221 attgcgccgg gaagttgacg tcattcaaag agtaaggccc gcaattgcct gcgtctgggt
     8281 tttgcgtact gcccttaggg aaggccgggt tcgtaaaggc cgcgtgcgaa ccaggcacgt
     8341 tcacgtttgc cggaaacgcg aagcttgagg tcacatcgtt gccgtactgt ggctggtagc
     8401 ggccggcaaa cgaacgatgg ttacccagta tctggccctg gtgatcgtag ttgatgccga
     8461 aattgacgtt gtagccgtca gacttgatat cgccgatgcc ggcaaacagc gaagcgcctt
     8521 cttccgaacc accgccggca tgcgtcggtg tgccgccatt ggcggagatg ctcaggcctt
     8581 ggaaatcact cttcaaaatg aagttcacga caccagcgat cgcgtcgcta ccgtagatcg
     8641 cggatgcacc gtccttcagg atttcgacgc gctcgatggc cgaaaccgga atcgaactga
     8701 tatccacgga ggtactggcc ccaccgacgg cgctgccgcc gtacgccgcc gagcgcagcc
     8761 cattgatcaa caccagcgtg cgctcactac ccagtccgcg tagcgaaata gtggaaatgg
     8821 caccggttgt aaatcctgtg gcctgcgaag cgactgtaga accgacgctg ctggcagccg
     8881 cgatctgtcg aaacagctgc tcgacgttgg tcgcaccggt gcgctgcacg tcttcagaac
     8941 tcaagatttg taccgggagc gacgtttccg tgactccctt gatcgaggta ccggtgacca
     9001 caatctcctg cagctctgca gaagcgtcag tcgcgttatc tgcggccatg ccgggtgcgg
     9061 cggcggcggc aatggctaag ctcacggcat aagcgacggc tttgctggtt ttcattgatt
     9121 tcccctgggt tgtatcccca acgcgcggct tcattgccgc ttgtcgcatc tgagccacat
     9181 ggcaggcaat gtggctgaat aagcagttta agtggactgt tttattttta caattgtgat
     9241 caatagtttc tgctttagaa ttttttattc agtgcgttac caatggtatg ttttccgggg
     9301 gaaaagccgc tgaatcaatt gagtcagcgc cgcgccttcg ggtcggacct gtgtcaatct
     9361 ggcgcgcgtc gcaggctgcg aaaagtagcc aatcgcctgc agccattctg agggccaccg
     9421 tacgtggcgc gtgcgaaaca ccatcggtaa tcgcgacgtt cgcgcctgct ctgaacaagt
     9481 cagctctgac gcgtcaggcc gcgcaacggg cccagatatt tctcgtagtg gcgccagcgg
     9541 ccgaccgagc tgtgatacat gctttgccga acttgccagg cgctggccgt tttgaccgcc
     9601 cggctcgtct gactgaagtt cagacactgc ggctgccaat cgagtcctac aaaatccaac
     9661 atctttcgaa tccactgctc cttgtgcgac accagctgct cgtagggcac ctcgagtatc
     9721 gcggcagacg gcagcaccga cttccagtgg cgcatcagca gctcatgttg ccggctgtag
     9781 tgagccagtt cagccaaatc aaacttatag ccatacgacg gggatagcgg ctggaaatag
     9841 cacgacaagc aggtatcgat cggattgcgc gtgacacaaa tgaatcgagc ccccggaaat
     9901 atcgtgtgaa tgagacctag gtaatccgca ttcacgttgg ccttgtccac cacaaccttg
     9961 ctgtccgcac cgtgtgtgcg cagcaatcgc aggtagcgct cgccgagctg gcgccgcacc
    10021 gactcgccga gcctccccga acgaatctcg gcctcgtacg cacgatacgc gtcgttccag
    10081 aacgtgagtt cgcccgcgcc tgtcgcccgc gggtgcgacg cgataatctg ctccaccagt
    10141 gaggttccgg atcgcatcat accgacgacg aacaccggtt tggcgcactc caacggcgtg
    10201 ctgacgcgtg acgggttggg cgaatagccg ccgatcagat cgcggaccgc ggcttcgcgc
    10261 gcgtacgggt cgtacttcgg ggccactttg cgccgcagct gattcgcgcg ttggtagtgt
    10321 ttgaacgccc gctcgtagtc gccgaggtca tcgaagtact tgcctagggc gaagcgcagc
    10381 tccgcttctt cctgcggctc aatcgcgctc gccagaatct cttcggcacc acgctgccat
    10441 tcgctgtcgg cggctgtcat cctgcgaagg cctgcaagtt ccgcccacgc cgccggcatc
    10501 ctgggcctcg attcgagcgc acggatgaac aattgacgtg cgtcgtcgaa acgaccgttc
    10561 agtcgctcaa tgcgtcccat acacaacagc gcttccgcgt cgcggggcgc caatcgaagc
    10621 accttcgtga gcagcgcttc cgcttcgctc acgcgagcct gcatgatcag aacattcgcc
    10681 agattgcact gcgcgcgcag gaacgttgac ttcgatttga tcgcgcggcg caggacagtc
    10741 tcagcttccg ggtactcgcc gcgcgaatag agcgcagcgc ccagattgca gagcgcttct
    10801 gcgttactcg gctgcagttc aaggacacgt cggaaatagt tctcagcctc gtcgtatgca
    10861 cccagcttcc actgtgcggc tccgaatccg tgccagccgt ccgcgttgcc gggctcctgc
    10921 tgaacgatct gcaggaaagc atcggccgcc tcgcggtgcc gaccttgcct cgcgaatttg
    10981 tgagcttccg ccagcagttc ctcggtcttg cggaataccg gtcgcttgtg acgtaccgct
    11041 tccacgggat cggaactggc ggcgtcccgt tgttcgctcg ccagctgcgt cagcaacaac
    11101 tgcatgagat gctcagcgag cgctggttcg atgccgttat ccaacagctt ggtcttgaag
    11161 gagctcgcaa gctttgcctt cttatagaga ttcagacgca acgacccggc ctggctcgcg
    11221 atttttttca gaaatgcgcg aagatgcttc gggtcctgct gtgcagcgga ctgatcggcc
    11281 cgccgcatcc ggatggtaac ggtcgatgcg acatcctggg cgagcgcaac gccgacgccc
    11341 gtagcttcgg ttgagtcaaa ccacgacaac atgctctagg gccttgtccc ggtgcaacag
    11401 ctgcaccctc caatgatggc tagatttacc gagcgaaaca ttaatttcca gggttcgaat
    11461 gcaatcccgt gcaaacagtc acagctccga tgccagactc gtcatatcga tctgacatat
    11521 cgtcaatcat aaattaatat taattaagaa agggttacga tcgttatcag cgcctgaccc
    11581 atccggctaa gcagtctcag gcctacaggc gatcgcgcgg ctcgataccc atctgccggg
    11641 caagcctcag gataaggtag tcgcccttgg caaattgacc gcgcagatga agttgtttcg
    11701 ctgctccatg tgcctcgttc tcggcgccac tggactgcgt gcgtcgttgg cgcaggccca
    11761 gaccccctca ccgctccagg agtggcagta ctcgggcggc gtgattctcg cccggttgtt
    11821 cgagccagcc ctgcctacct ttagaaccat caccggactg gcagccgagt tgcaacccgc
    11881 gtatgacggg tcacgcgcct atcggctcga aggcggcccg gacgtcaatg tctactatcg
    11941 tgacgtcgcg ttcatcagca ccggggacgg gatcggttat aacgtgctgc gtggcagtca
    12001 ctatcaggtc ggcgtcgcga tgacctacga cctcggccgc agggtggatc aggacgatgc
    12061 caatctgcac ggcatgggaa atatctcccc cgctccggtg gccaagctct acgcatcgtg
    12121 ggtgctatcg aagaagctgc cgctgatcct gcgggtgagc gcacggcagt tcatcggcgg
    12181 tgcgcagggc gccgtcggcg acatcgcaat ctataccccg ctgcccggca gttcgcacac
    12241 tttcgtgatg ttcgcgggac cgtcgctgac tctggcgacc aatcactacc ttcaggtgct
    12301 gtacggtgtg acgcctctgc aatcccgcgc gtcgggtcat ccgatctacg cgatcgccca
    12361 cgacggaacg gctgcggccg gcgtcgggtt cagcgcgacg aaattctttg gctcacactg
    12421 gctcatcaat ttcgacggtg cgctcagtca aatccgtgga aagcctgcgc gcagcccggt
    12481 ggtcgagaag atcaatcagc acgtggcggt gctgtcgctc aactatcagt ggtgaagcgt
    12541 cgccgggttt cgagtctcag ggggtcggca cgtccgagat gatcccgagg cgcttgtatt
    12601 cctcggccac acgcggccac agggtaaccg cctgcgcgat atccgcatta ccttgctgcg
    12661 ccctgcctga tcgcaacttg tcgatgcccc gtccatacca tgcccaggcg tctttgggat
    12721 tcaacttcac agcggtatcg tagtcggcaa tcgatttgtt gaagtctccc attctcagca
    12781 gaataaggcc gcgactgtcg gcgatcctcg catagaggga gctcgactta tccgtcagcc
    12841 gcattgccga attgcaatcc ttgagtgcca gtggaaagtc cattcctccc agtcctctga
    12901 tccaacagcg ctcattcagg acgttgggca gccgtgcatc ctcgccgtgc gagtcgatcc
    12961 acagattcat ctgactgatt gcaggatcaa actgatcggc gtgttcgtag gccagcgcca
    13021 tccgaaagcg ttcattggcc tgtttcggtg cgatcgcatc ggcggcatcg agatccatac
    13081 ggccaccagg cttgtcaccc gtgcgaatct tcagttcggc gcgcgccagc agtgtgttca
    13141 gatcgttcgg tcgcagctgc agtgagcgat cgaaatccgc catcgcgggg gcgagttgct
    13201 tcagctcctc atgcaggagc ccgcgccgat agacatattc cgcgttgtcg ggcgccagat
    13261 cacaggcacg gttcagcgcg gtcagggcct gatcaaagtc acgacgcgat tcgaacgcct
    13321 caccgcggcg tgcatagtct gcaggatcgt ctgcatcgga ggcgaccgca gttggactgg
    13381 cctcttcctt ggggtttgga accgtggacg cagcgacgtc cgttggtcca tcaggggcgg
    13441 ttaccggagc cgtcgcggcg gcggactgcg cactggaagc ggctgtattc ttgggctt
//
LOCUS       DATMRM010000218        17592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1059295, whole genome shotgun sequence.
ACCESSION   DATMRM010000218 DATMRM010000000
VERSION     DATMRM010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17592
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059295"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1413)
                     /locus_tag="VNZ06_06375"
     CDS             complement(<1..1413)
                     /locus_tag="VNZ06_06375"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWW20412.1"
                     /translation="MTDANSDNGVESLAIWRGAVASKTKLTMEGSMKSSSVPLRRAVT
                     LALAISGAMASGYAWSAGEANANTNASPELDEVIVTAEKRSENLQDVPISVIAVSSQQ
                     LKDAGVKDIKDLQTLTPGLTVTSDSAESTTVARIRGIGTVGDNPGLESSVGIVIDGVY
                     RPRNGVGFGDLGELEQVEILEGPQGVQFGKNNDAGVINITSKRPSQTFEATGEITGGN
                     FSDREVNASVTGPIAEGIAARLFVEYQRRDGWMQLLNGEGPLSQHSTDDRNVWVARAQ
                     FLFTPTDNIDFLLIADVSKRNETCCGAVPIDTGPFEPIINAIAFANNGGANGSGISLT
                     PTKYTAWANQPIRAFVRDYGFSGELNWNLDWAKLTSITAWRDNTQGGGNDFDYTGIDL
                     LQSPPTAFNRTDFKQFSEELRLAGKAGPLQWLVGAFYAKESLQPSGQLYQGTDWEAYL
                     SLVASSAVGAPNPGVIAGLGG"
     gene            complement(1479..2621)
                     /locus_tag="VNZ06_06380"
     CDS             complement(1479..2621)
                     /locus_tag="VNZ06_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWW20413.1"
                     /translation="MDFSYTEEQLALQETLQRFVSREYDFETRRNLSSSDLGFSADAW
                     KQYADLGLLSLPIPEEYGGLGGNAVDIMLIMEMVGRGLLLEPYFSSVVLSGCLIRDAA
                     PAAMKQAVLPAIAAGKLKVAFACYEAAGRYDLAHVATRAVSDGKGGWRLTGDKSVVQD
                     GPSADRYLVSARISGSLTDRDGLALFLVDRNAPGVSLTGYTTQCGRRAADVRFENVVV
                     GADAMIGGPGKAIHIIERGVDRGIAALCAEAIGIMTALNEATLNHLKTRKQFGVPIGK
                     FQALQHRMADMFIATEQARSMAIIAAVHADSDDAAARQRAISGAKTYIGQAARLVSQQ
                     AVQLHGGMGLVDEMIVSHYFKRLTMINIGFGDADYHMARFSSALEA"
     gene            complement(2632..3843)
                     /locus_tag="VNZ06_06385"
     CDS             complement(2632..3843)
                     /locus_tag="VNZ06_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWW20414.1"
                     /translation="MDLKYSQEEEAFRAQVRAFLEAELPSDIREKSRLGKRLAKEDFI
                     RWQRILNAKGWGAVMWPQKFGGTGWNIVQQHIFEEERVEAGAPPQIAFAFKMVSPVLM
                     AFGNPQQQEYFLPRILSGEHWWCQGYSEPGAGSDLASLKTSAVRSGDHYIVNGQKTWN
                     TLGQYADWIFCLVRTSTEGRPQEGISFLLIDMRTPGITVRPITLLDGEHEVNEIWFDN
                     VSVPLTNRVGEENQGWTYAKFLLNHERAGNAGVGTCKRALKKLKQIASEQMANGKPLI
                     QDSRFRDRIAQVELELMALEITNLRVLSSMVNEKQALGPEVSVLKIKGSEIQQQIAEL
                     QMSALGHDALPYFHDGLDIGWVVDPLLGEHYRPYAPPLSGQYFNLRKTSIYAGSNEVQ
                     KNIISRHILGL"
     gene            complement(4184..4993)
                     /locus_tag="VNZ06_06390"
     CDS             complement(4184..4993)
                     /locus_tag="VNZ06_06390"
                     /inference="COORDINATES: protein motif:HMM:NF004855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWW20415.1"
                     /translation="MQERTNPVLEMGARGALPEPPKFATLQEERLHRKQRLAAAFRLF
                     SKFGFDEGVAGHITARDPEFPDTFWVNGFGVHFSQICVSNLIRCDHEGKVVEGGHPVN
                     TAAFAIHSRVHQARPDAVGAAHSHSTYGRAWSTLGRRLDPITQDVCAFYEDHALFEDF
                     TGVILDADEGTRIAKALGKNKAAILQNHGLLTVGQTVDEAAWWFITLERSCHVQLLAE
                     AAAARAGVAPKHVSKEAAQQTYQVIGSPFAGWFQFQPLYARIVQEQPDLLD"
     gene            5184..6428
                     /locus_tag="VNZ06_06395"
     CDS             5184..6428
                     /locus_tag="VNZ06_06395"
                     /inference="COORDINATES: protein motif:HMM:NF022732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HWW20416.1"
                     /translation="MVRMIIAARFALAASICYAACASGQSTAAPAAAPAATPAAPAAA
                     PAMAPVEPSNTDYSKPAVWLCWPGRQDACTQPQDATIVAENGTLKHEAYHAAKNPAID
                     CFYVYPTVSTQTTGNSDLTVTHAETSVVLAQFARFGEQCRLYAPMYRQVTLTALRAGI
                     AGQPISIDRNLGYSDVLAAWRYYLAHENKGRGVVLVGHSQGSGVLTRLVREEIDGKPV
                     QSQMISAILMGTVLPVPKGADVGGAFKHIPLCHSNSQIHCVIAFADFRANVPPPSNTR
                     FGKVRDEGMQAACTNPAALRGGSGKLDAYLSSRSITATEPAAQQASWTVPQQTIDTPF
                     VKVPGMLSAECVNNEHGSYIAVTVHPSSTGKRTNEITGDVVANGKVLEDWGLHLIDAN
                     LHMGNLVAIVGTEARTYKAAAH"
     gene            complement(6463..7065)
                     /locus_tag="VNZ06_06400"
     CDS             complement(6463..7065)
                     /locus_tag="VNZ06_06400"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWW20417.1"
                     /translation="MSKLILVRHGESHGNRDRIFAINPAELPLTELGYRQASEVADQI
                     ELMFKAEVVVASPFLRARETARVIAGRLRLPLEIETDLYERDVGALKGQSYDELERSP
                     GYSTSTPWAWRPEAGESYEDVRARVGPILDRLARAHPTRDVVIVSHGGVMQVLQAYVT
                     GDWRNLHAPPNCGMVIIEHSPEGYSTPQIVGDVCATDAGG"
     gene            complement(7108..7263)
                     /locus_tag="VNZ06_06405"
     CDS             complement(7108..7263)
                     /locus_tag="VNZ06_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20418.1"
                     /translation="MAQVKIYGLREHLEPVRARLCDVIHSCVVDALQFDERAHRFFHL
                     EPRFGGR"
     gene            complement(7232..7873)
                     /locus_tag="VNZ06_06410"
     CDS             complement(7232..7873)
                     /locus_tag="VNZ06_06410"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HWW20419.1"
                     /translation="MLIAIVTFDGFNEIDSMVAMNILGRALRPVGRVVIASSTPKITS
                     MNGVRLHAQATLAEARSADAVIVGSGVRTLEIAEDAELMSQLKFDPHRQLIAAQCSGA
                     LLLAKLGLLDGLPACTDSSTKPWVEKAGIQVLDQPFNARGNVATAGGCLASVYLAGWI
                     IARAQGLGAVRSVLSYVAPVGEKDEFVARAVNAIAAYLPTGQAHGASENLRTA"
     gene            complement(7893..9269)
                     /locus_tag="VNZ06_06415"
     CDS             complement(7893..9269)
                     /locus_tag="VNZ06_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HWW20420.1"
                     /translation="MSSVESTAATPVPQRPHILQLALPSIAANLMFSMVALVQTKFVG
                     GLGASAVAAVGAGQRVFFALQAVFTAIGVGTTALVSRSWGAGQREEAGRITITSLVVG
                     AAAGAIITLTGMLFSRNIADLFGLDEYTANLASRNIFWLSAFICGFAIDIIFSGALRA
                     AGNVWTPLVFGLAVNLVNLPLLYAFIFGKLGAPRMGPAGAAFASGLSLTLCGSVLLLL
                     WSRQRLTIKFLSSDLWRLDRYRHLFHIAYPVAIEQLVLQAGFFLFLSLIGRFYGTEAF
                     AAYNVGVNMLNAAMVIGTGFSIAGSTLVGQNLGAGDLEAAARSGWRACALAVSAMAMV
                     GLIVVLNAPGLAHFFLGTDQVGVQRATEFTYILAAMMPLLGVDMAIGGSLRGAGDTRF
                     PLMSTFLGLIGMRCGLAALFTAMHLPVVWVYSSIIGDYVLKGSLLIWRFKSGRWKHAI
                     HARLAAPL"
     gene            complement(9357..9731)
                     /locus_tag="VNZ06_06420"
     CDS             complement(9357..9731)
                     /locus_tag="VNZ06_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20421.1"
                     /translation="MKKIRNIFLAPLLAAFGFLMSTAAFADVSGNWLMTVETQAGTGS
                     PTFTLVQKGDQVTGTYKGQLGEAPVTGSVKGNVITLSYKINAQGQDLEVVYTGTLDGD
                     KITDGKVKLGSFGDGTFTGKKQ"
     gene            9885..10301
                     /locus_tag="VNZ06_06425"
     CDS             9885..10301
                     /locus_tag="VNZ06_06425"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HWW20422.1"
                     /translation="MGIALLALGTTGTPAVAHHAFQAEFDGNAPVMLQGKVMKVEWIN
                     PHTWVHMEVITDGQPQEWMVEAGTPNTLLRAGINRDTLKPGEEIIVRGYRAKDTRCRP
                     ACKANGRDVTFTDGRKVFMGSSGTGAPRDGSDPNEK"
     gene            complement(10338..11498)
                     /locus_tag="VNZ06_06430"
     CDS             complement(10338..11498)
                     /locus_tag="VNZ06_06430"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWW20423.1"
                     /translation="MTYAVDRPYYDADSHIMETPEWIASFVDPELLPRLPEMSLLKSG
                     TKSFDVIRAAINAQKDRASAGVQAHDVVKGPKGWQAPGAFDAGERAAALDDLGFRKQL
                     VFSTFSGTQYLHHEDLDLRYGGIRAHNRAMSAFCGKDSRLLAVGQLSLADPARCVEEL
                     KEGVRLGCKTFWVPGQPVGDKSPGHPDFDGVWKAFCDLDVPFMLHVGPNSSAKLSAYE
                     NNGQPRPKDITGAEGGENLRVRDFMLLSIGPQQFLTALVFDGVLERFPKLRGGVIELG
                     AGWVPQYLRTLDLSQRIFKRTDPSVAALSLKGSEYIRRAIKFTPFPGEDVGRLVRDAG
                     ADLFLFSSDYPHPEGTDDPVGRFERTLEQTSETDKHKFYTTNFQEMMGAASR"
     gene            complement(11552..12247)
                     /locus_tag="VNZ06_06435"
     CDS             complement(11552..12247)
                     /locus_tag="VNZ06_06435"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HWW20424.1"
                     /translation="MAKSFEVVFALYPRITQLDFTGPYEVFSRLPGARCTLASTGGGT
                     IQAEGGLAFANVVPLASVQQCALLCVPGGFGTVQALDDREYLSQLRRLASCARYVTSV
                     CTGSLLLAAAGLLRGKRAACHWAWRHLLTEFGVQPDPARIVRVGRTITGGGVTAGIDM
                     ALTVVAEIAGAASAQALQLGIEYAPEPPFDCGRPERAPAAVLAAARQRLETVSADRDI
                     AVRRAAQLDLAAT"
     gene            12327..13268
                     /locus_tag="VNZ06_06440"
     CDS             12327..13268
                     /locus_tag="VNZ06_06440"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HWW20425.1"
                     /translation="MTRNISFLIYPQFQLLDAAGPISAFEVAGRIRPGAYALRVIALQ
                     PGLVKSSSGIPLPAMALGAVHRVDTLIVSGGDGSREAAQCPRTQRFIQACARSARRTS
                     SVCSGTYLLAAAGLLDGKLATTHWSCTTDFRSRFPKVRLDPDRIFVREGRVWTSAGVT
                     AGIDLALALIEQDLGEKIARATARQLVVYHRRPGGQSQFSALLQMECSSGKFAPLLDE
                     VRSSLRHRHCVAELAERVCMSPRQFFRAFQADTGMTPAKAVERLRADAARAELSGSAR
                     SVQDVARSCGFGSAERMRRTFLRLYGVSPIGVKQRAY"
     gene            complement(13271..13702)
                     /locus_tag="VNZ06_06445"
     CDS             complement(13271..13702)
                     /locus_tag="VNZ06_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20426.1"
                     /translation="MLIVPSVRSVAAAITAVVALAACASSHVLVGAPRPPISPDQVTI
                     YTNPPAQYEQIAILNTSSRGSFALTAQGKTDKVIQRLKDEAAKLGANGVLLQGIGDRQ
                     SGSIGSGFGGGSGNVGVGFGTSVGTYQKTGNGVAIYVGPAH"
     gene            complement(14017..14523)
                     /locus_tag="VNZ06_06450"
     CDS             complement(14017..14523)
                     /locus_tag="VNZ06_06450"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HWW20427.1"
                     /translation="MKRAFIGWLIGIVPLLALGADSNPDESFYKSAGEGGLAEVAVAK
                     LAEAKASSAALRDFGGMMVKDHSTADQKLWATAAHKGVKMPTTPSVEQVAAGDRLKLL
                     SGDSFDKAYIKNQIESHRDALALFKKEIASGRDKDASQFAQTMLPTVQAHLDKIMKIA
                     STAGVAVE"
     gene            complement(14674..15372)
                     /locus_tag="VNZ06_06455"
     CDS             complement(14674..15372)
                     /locus_tag="VNZ06_06455"
                     /inference="COORDINATES: protein motif:HMM:NF021385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HWW20428.1"
                     /translation="MSTPNDSHWSALSGTLDVQGWAVLKQLLAPPKCDDIAALYDEER
                     YFRSRVIMTRHGFGRGEYKYFRYPLLPVIQQLRTEIYPHLTALANAWNARMKLDVRFP
                     YCHVEFLDRCHRAGQTLPTPLLLKYGEGDYNCLHQDLYGEHVFPLQLAILLSEPGRDF
                     TGGEFVLTEQRPRMQTRATVAPLKQGDALVFAVRQRPVRGSRGDYRVNQRHGVSKLLS
                     GARFTLGVIFHDAA"
     gene            complement(15369..16454)
                     /gene="ada"
                     /locus_tag="VNZ06_06460"
     CDS             complement(15369..16454)
                     /gene="ada"
                     /locus_tag="VNZ06_06460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HWW20429.1"
                     /translation="MQAQITRQAAAIKTLNDPRWSAVVARDRRQDGKFFYAVKTTGVY
                     CRPSCPSRRANPGNIAFYATTAAAEAAGFRACKRCRPDRPPLEVQHAATVARACRMIE
                     SAEEAPSLAVLAKDSGISPYHFHRLFKAVTGVTPKEYANAHRTRRVQRSLQTGSSVTE
                     AIFDAGFNSNSRFYEQANAMLGMSPSQFRRRGADEQIRFAVGQSSLGAILVAVSAKGV
                     CAITMGDDPNQLVRELQDRFSNATLVGSDEGFESLVAQVVGLVEAPSLGSNLPLDVRG
                     TAFQRRVWKALSKIPAGSTVSYASIAKKIGAPQSVRAVAQACGANPLAIAIPCHRVVR
                     TDGALSGYRWGIERKRTLLTREGVSRP"
     gene            16538..17329
                     /locus_tag="VNZ06_06465"
     CDS             16538..17329
                     /locus_tag="VNZ06_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWW20430.1"
                     /translation="MPEQIRFHRFPVMGIEAMSASTSHVFPRHTHDRYGIGVVDAGGH
                     ASLSDRGQVEAGPGGLICVNPGEVHDGRPSGGVSRSWRIVYLEPTLMRGRCREVSEGR
                     SATLYFAAPVFADETLRALFNQTFVSTESMACESGLLMIVARMRARWTVDRAPSRTTG
                     CIRRARERIDSNPAEPHTLSDLANEGGLSRYQLLRSFARELRLTPHAYLLQQRIALAH
                     RLIRQGGRPQAEIAIASGFFDQSHFSRCFARQFGVSPRRYAAQRA"
     gene            17386..>17592
                     /locus_tag="VNZ06_06470"
     CDS             17386..>17592
                     /locus_tag="VNZ06_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20431.1"
                     /translation="MHPSASIRRCRDEERVIILQICNQAAEAYRHTIPRDCWKEPYMP
                     QDELDQEIANGVRFWGYESEQGLSG"
BASE COUNT         3395 a         5649 c         5217 g         3331 t
ORIGIN      
        1 gccgcccagt ccggcgatga caccagggtt cggggcccct actgcgctgg atgccaccag
       61 gctcaggtac gcttcccagt cggtgccttg atacagctgc ccgctcggct ggagggactc
      121 cttcgcgtag aaggcaccca ccaaccactg caacggaccc gccttgccgg ccagccgcag
      181 ctcttcgctg aactgcttga agtctgtgcg gttgaaggcc gtcggcggcg actgcagcag
      241 gtcgatgccg gtgtagtcga agtcgttgcc gccgccttgc gtgttatcgc gccaggccgt
      301 gatcgacgtc aatttggccc aatcgaggtt ccagttcaat tcaccggaaa agccgtagtc
      361 acgcacaaac gccctgatcg gctggttggc ccacgccgtg tacttcgtcg gcgtcagcga
      421 gatgccgctg ccattcgcgc ccccattgtt cgcgaacgca atggcattga tgatgggttc
      481 gaacgggccg gtatcgatcg gcacagcgcc gcagcaagtc tcgttgcgct tggagacgtc
      541 ggcgatcaac aggaagtcga tgttgtcggt cggcgtgaac aggaattgcg cgcgcgccac
      601 ccacacattg cgatcgtcag tgctgtgctg gctgagaggc ccctcgccgt tgagcagctg
      661 catccagccg tcgcggcgct ggtactcgac gaataagcgg gcggcaatgc cttcagcaat
      721 cggtcctgtg accgaggcgt tgacttcgcg atcactgaag ttgccgccgg tgatctcacc
      781 ggtggcttcg aaggtctgag acggccgctt gctcgtgatg ttgatcacgc cggcgtcgtt
      841 gttcttgccg aactgcacgc cttgaggccc ttcgagaatt tcgacctgtt cgagctcgcc
      901 caggtcgccg aagccgacac cgttacgtgg tcggtaaacg ccatcgatca cgatacccac
      961 cgacgattcc aggcccgggt tgtcgccgac cgtgccgata ccgcgaatgc gcgccacggt
     1021 cgtgctttca gccgaatccg acgtcacggt caaacccgga gtcagcgtct gcaggtcttt
     1081 gatgtccttc acgccagcgt ccttgagctg ttgtgaggac actgcaatca ccgagatcgg
     1141 aacgtcctgg aggttctccg agcgtttttc cgcagtcacg ataacctcgt ccagctctgg
     1201 agaggcattg gtgttggcat tcgcctcgcc agcgctccaa gcgtaaccgc tcgccatggc
     1261 accggagatc gccagggcca gggtaacggc tcgtcgtaaa ggtacacttg aactcttcat
     1321 actcccctcc atagtcaact ttgttttcga agcaaccgca ccgcgccaga ttgccaaact
     1381 ctccacaccg ttatcactat tggcatcggt cattatttcc gcccctcgaa tatgtgtcgc
     1441 aaatagacaa aaccggttgt cgtaggtggt ctgcgccgtc aggcctcgag tgcactgctg
     1501 aagcgcgcca tgtggtaatc cgcatcgccg aagccgatgt tgatcatggt cagtcgcttg
     1561 aagtagtgac tgacgatcat ttcgtccacc agacccatgc cgccgtgcag ctgcacggcc
     1621 tgctggctca ccaatcgtgc ggcctgtccg atgtaagtct tcgcacccga gatcgcgcgt
     1681 tggcgtgccg ccgcgtcgtc ggaatcggca tgaaccgccg cgatgatggc cattgaccgc
     1741 gcctgctcgg tggctatgaa catgtcggcc atgcggtgtt gcagagcctg aaattttccg
     1801 atcggcacgc caaactgctt gcgcgttttc agatgattca gtgtggcttc gttgagcgcc
     1861 gtcatgatcc cgatagcttc ggcgcacagc gcggcgatgc cgcgatcgac acctcgctcg
     1921 atgatgtgga tcgccttgcc cggaccgccg atcatcgcgt cggcgccgac gactacgttc
     1981 tcgaagcgca cgtcggcagc ccggcgtccg cattgagtcg tatagcccgt gaggctgacc
     2041 cccggcgcgt tgcgatcgac caggaacagc gctaagccat cacgatccgt caggctaccc
     2101 gagatcctcg ccgacacgag gtatcgatcg gcggatgggc catcctgcac gacgctcttg
     2161 tcgccagtga gtcgccatcc gcctttgccg tcgctgaccg cgcgggtggc gacatgcgcc
     2221 agatcgtagc gtcccgccgc ctcgtagcag gcgaaggcca ctttcagttt gcccgccgcg
     2281 atcgcgggca acacggcctg tttcatggcc gccggtgcgg cgtcccgaat cagacagccg
     2341 ctgagcacca cgctgctgaa gtaaggttcg agcagcaggc cacgaccgac catttccatg
     2401 atcagcatga tgtcaaccgc attgccaccc aggccgccgt actcctcagg gatcggcagc
     2461 gacagcaggc ccaggtccgc gtattgcttc caagcgtcgg cagagaagcc gaggtcggag
     2521 ctcgacaggt tgcgacgtgt ttcgaagtca tattcacgcg agacgaagcg ctgcagggtc
     2581 tcctggagcg caagctgttc ttcggtgtaa ctgaaatcca tcgacgcgtt cttacaggcc
     2641 aagaatgtgg cgcgaaatga tgttcttctg aacttcattc gagccagcgt agatcgaggt
     2701 cttgcgcagg ttgaagtact gcccggacag cggcggcgcg tacggccggt agtgctcgcc
     2761 cagtaacgga tcgacgaccc atccgatatc gagcccgtcg tgaaagtacg gcagcgcatc
     2821 gtgtccgagc gccgacatct gcagctcggc gatctgctgc tggatctccg agcctttgat
     2881 cttcagcact gagacttcag gaccgagcgc ctgcttctcg ttgaccatgc tggacagcac
     2941 gcgcaggtta gtgatctcga gggccatcag ttcgagctcg acctgcgcga tacgatcgcg
     3001 gaagcgcgag tcctgtatca ggggcttgcc attcgccatt tgctcgctgg cgatctgctt
     3061 gagcttcttg agggcacgct tacaggtgcc gacgccggcg ttgccggcac gttcgtgatt
     3121 gagcagaaac tttgcgtatg tccagccctg gttctcttct ccgacgcgat tggtcagtgg
     3181 caccgagacg ttgtcgaacc agatttcgtt gacctcgtgt tcgccgtcga gcagcgttat
     3241 cggtcgcacc gtaatacccg gagtcctcat gtcgatcagc agaaaagaga tgccttcctg
     3301 cgggcgaccc tcggtgctgg tccggaccag acagaaaatc cagtcggcgt actggccgag
     3361 cgtgttccag gtcttctgtc cattgacgat gtagtggtcg ccggaacgaa ccgcgctggt
     3421 tttgagcgag gcgaggtccg agccggcgcc gggctcggag tagccctggc accaccaatg
     3481 ctcacccgac aggatgcggg gcaggaaata ttcctgctgc tgaggatttc cgaacgccat
     3541 cagcaccggt gagaccattt tgaaggcgaa cgcgatttgc ggtggcgctc cggcctccac
     3601 gcgttcttct tcgaagatgt gctgctgaac gatgttccag ccggtgccgc cgaatttctg
     3661 cggccacatg acggcgcccc aaccctttgc gttcagtatt ctttgccagc ggatgaagtc
     3721 ttccttggcc agccgtttgc ccaagcgcga cttctcgcga atatccgacg gtagctccgc
     3781 ttccaggaac gcgcgcacct gcgcgcgaaa agcttcttct tcctggctgt atttcaaatc
     3841 catcgaatat ttttcctcga aggattgctg cccgctgcag ctcaccctcc gaaacacccc
     3901 cggccgcagg ccggatattc cggccgtcct caagccaaac taaatatagc ttaaggtgtt
     3961 gctatattgt ggcgtagtcg ccgagggtgt ttagcaattg gcgccaacct cttcacttca
     4021 agcgccaatc atctgttgcc gatacacaac agcagtgtac taaatctagt aaattcaaca
     4081 tttgcgcggt tcactaattt gaccgcgcaa tcgttccacg ttttcgctgt tcagtctcgt
     4141 cgcaccgtgt gtttgtggca acggccgcgg tactttttac gactcagtcc agcagatccg
     4201 gttgctcctg gacgatccgc gcgtacagcg gctggaactg gaaccagccc gcgaagggtg
     4261 agccaattac ctgataggtc tgctgcgccg cttccttcga tacatgtttc ggggcgacac
     4321 cggcgcgggc ggcggccgct tcggccagca gctggacgtg acacgaccgc tcgagcgtaa
     4381 tgaaccacca cgccgcctca tccacggtct gtccaacagt cagcaagccg tgattctgaa
     4441 gaatggcagc cttattctta ccgagcgcct tggcaatgcg ggtaccctca tccgcgtcca
     4501 gtatcacgcc cgtaaaatcc tcaaaaaggg catgatcttc gtagaatgcg cacacatcct
     4561 gagtgatcgg atccagacgc cggcctaagg tggaccacgc gcggccgtag gtcgaatgcg
     4621 agtgcgcagc gccgaccgcg tccgggcgcg cctgatgcac ccgcgagtgg atggcaaacg
     4681 ctgcggtgtt cactggatgg ccgccttcca ccaccttccc ctcgtgatcg cagcgaatga
     4741 gattagagac gcaaatctgg ctgaagtgca ccccgaagcc gtttacccag aaggtatcgg
     4801 ggaattcggg gtcgcgcgcg gtaatgtggc cagcgacacc ctcatcgaac ccgaacttgg
     4861 agaacagtcg aaacgctgca gcgagccgct gcttgcgatg cagacgctcc tcctgaaggg
     4921 tcgcgaactt gggaggctcc ggaagcgccc cgcgtgcgcc catttccaaa accggattcg
     4981 tccgctcttg catcgcagca ttcattccag ttggcactcc ttttgtcttg agctgattct
     5041 atcgccttcg cggcgaggcc gccgcccgcg ggcgcgaacc ttgtttccaa ttgtaaggtg
     5101 tcatgtttag tcaacccgag cgtagaagtg aatgcgatat aattttctca accaaaaacc
     5161 gtcaaacctg tcgggggatt tcaatggtcc gcatgataat cgcagcgcgt ttcgcgctgg
     5221 ccgcgagtat ctgttatgca gcgtgcgcgt cagggcaatc aacagccgca ccggcagccg
     5281 caccggcagc cacaccggct gcgccggcag ccgcgccggc gatggcgccg gtcgagccgt
     5341 ccaacaccga ctattccaag ccggcggtat ggctgtgctg gcccgggcgc caggatgcat
     5401 gcacccagcc gcaggatgcg accatcgtcg ccgagaacgg cacgctcaaa cacgaggcct
     5461 atcacgccgc caagaacccg gcgattgatt gcttctacgt ctatcccacc gtatcaacgc
     5521 agaccaccgg caactccgac ctaaccgtca cgcacgcgga gacctccgtg gtactggcgc
     5581 agttcgcccg cttcggcgag cagtgccggc tgtacgcgcc gatgtaccgg caagtgaccc
     5641 tgacggcatt gcgcgcggga atcgcaggtc agccgatttc gatagaccgc aatctcggct
     5701 acagcgacgt gctcgctgcc tggcgctact acctggcgca tgagaacaaa ggccgcggcg
     5761 tcgttctcgt cggacacagc cagggatcag gcgtgttgac ccgcctggtc cgcgaagaaa
     5821 tcgacggcaa gccggttcaa tcgcagatga tttctgcgat tctcatgggg acggttctgc
     5881 cggtgcccaa gggcgccgat gtcggtggcg ccttcaagca catcccgctg tgccactcca
     5941 acagccagat ccactgcgtg atcgcattcg ccgatttccg cgcgaatgtg ccgccgccgt
     6001 ccaatacgcg cttcggcaaa gtccgggatg aaggaatgca ggcggcctgt accaaccccg
     6061 cggcgctccg cggcggctcg ggcaagctcg atgcgtacct gtcttcgcga agcatcacag
     6121 ccaccgagcc cgctgcgcaa caggcatcct ggacggtccc acagcagacc atcgacacgc
     6181 cgtttgtcaa agttccggga atgttgagcg ccgaatgcgt caacaatgaa catggttcct
     6241 acatcgctgt gaccgtgcat ccgtcgagca ccggcaagcg caccaatgaa atcaccggtg
     6301 atgtcgtggc caacggcaag gttctggagg attggggtct gcacctgatc gacgccaacc
     6361 tgcacatggg aaatctggtc gcgatcgtcg gtaccgaggc caggacctat aaggccgcgg
     6421 cgcactagtc ttcagcttga agcaacgcac tgacgcgggg cgtcacccgc ccgcgtcagt
     6481 cgcgcagaca tctccgacaa tctgcggcgt tgaatatcct tcgggggaat gttcaatgat
     6541 caccatgccg cagttcggcg gtgcgtgaag atttctccag tcgcccgtca catacgcctg
     6601 cagtacttgc atcacgcctc cgtgactcac gatcacgacg tcgcgagtag ggtgtgcccg
     6661 cgcaagccga tccaatatcg gcccaacgcg ggctctgaca tcctcatagc tctcgcccgc
     6721 ttccggacgc caggcccacg gggtgctcgt gctatagccc ggcgagcgct ccagttcatc
     6781 gtacgactgc cctttgagcg caccgacgtc acgctcatag aggtccgtct cgatctcgag
     6841 cggaaggcgc agtcgcccgg cgatgacgcg cgcggtctcg cgcgcccgca ggaaggggct
     6901 cgcgacaacc acttcggcct tgaacatcag ctcgatctga tcggcgacct cactcgcctg
     6961 ccgatagccg agttcggtca gcggcagctc cgcaggattg atggcgaaga tgcggtcacg
     7021 gttgccatgg ctttcgccgt gccggaccag aatgagctta ctcatgccga ccgtatctag
     7081 acttcaacgc ggtagttgaa accgatctca tctcccgcca aaccgcggct ccaggtggaa
     7141 gaaccgatga gctcgctcgt caaactgcag tgcatccacc acgcacgaat ggatgacatc
     7201 gcagagccgc gcgcgcaccg gttccagatg ctcacgcagt ccgtagattt tcacttgcgc
     7261 catgggcttg tcctgttggc aaataggcag cgattgcatt caccgcgcgc gcgacaaact
     7321 catccttctc tcctaccggg gcgacgtagc tcagcaccga tctgacagcc cccaaacctt
     7381 gggctcgggc aatgatccag cccgccagat acaccgaggc tagacagcct cccgcggtgg
     7441 cgacattgcc gcgcgcattg aacggctgat caagcacctg gatgccggcc ttttcgaccc
     7501 agggtttggt gctcgagtcg gtgcacgcgg gtagcccgtc aagcaatcca agctttgcca
     7561 gcagcaacgc acccgaacac tgggcggcga tgagctgacg gtgcggatca aacttgagct
     7621 gggacatcag ctcggcatcc tccgcgatct cgagcgtgcg cactccgctg ccaacgatga
     7681 ccgcgtcggc ggagcgcgcc tcagccagcg tcgcttgtgc atgcaggcgc acgccattca
     7741 tcgacgtgat cttcggtgtg ctgctggcga tcacgacgcg gcccacgggt ctcagtgcgc
     7801 gcccgagaat gttcatcgcc accatcgaat cgatctcgtt gaatccgtcg aacgtcacga
     7861 ttgcgatcag catgtgtgcc tccgttgcgc cttcaaaggg gagccgcgag ccgcgcgtga
     7921 atcgcgtgct tccagcgtcc ggatttgaat cgccagatca acagcgaacc cttgaggaca
     7981 tagtcgccga tgatcgagct atagacccac acgaccggca gatgcatcgc ggtaaacagc
     8041 gccgccagac cgcaacgcat gccaatcagg ccgagaaacg ttgacatcag cgggaatcgc
     8101 gtatcgccgg cgccacgcaa tgagccgccg atcgccatgt caacgcctag cagcggcatc
     8161 attgccgcca gtatatatgt gaactccgtg gcgcgctgta cccccacctg atccgtgccc
     8221 agaaagaagt gcgccaatcc gggggcattg agcaccacga tcaggccgac catcgccatc
     8281 gcgctgaccg cgagagcgca cgcgcgccaa ccgctgcgcg ccgcggcttc cagatcccct
     8341 gcgccgagat tctgtccgac cagcgtcgaa ccggcgatcg aaaagccggt gccaatgacc
     8401 atcgccgcgt tgagcatgtt cacgccgaca ttgtaggcgg caaaggcttc ggtaccgtag
     8461 aaacgcccga tcagcgacag gaacaggaag aatccggcct gcaataccag ctgctcgatg
     8521 gcaaccggat aggcgatatg aaacagatgg cgatagcgat cgagccgcca cagatcgctg
     8581 ctcaggaact tgatcgtcag tcgttgccgc gaccatagca gcagcagtac gctgccgcag
     8641 agtgtcagcg acagacctga ggcgaacgcc gctccagcgg gccccatgcg cggcgcacct
     8701 aacttgccga agatgaacgc gtacagcaat ggcaggttta ccagattgac ggccagtccg
     8761 aacaccaggg gcgtccatac attgccggca gcacgcagcg caccggaaaa aatgatgtcg
     8821 atcgcgaagc cgcagatgaa ggccgacagc cagaagatgt tgcggctcgc caggttcgcg
     8881 gtgtactcat ccagcccgaa caggtcggca atgttccgcg agaacagcat gccagttagt
     8941 gtaatgatcg cgccggcggc ggcgcccacg accagcgagg taatcgtgat ccggcccgct
     9001 tcctcgcgct gaccggcgcc ccaggagcgc gacaccagtg ccgtcgttcc cacaccgatg
     9061 gccgtaaaca cggcttgcaa cgcaaagaac acgcgctggc cggcgccgac cgcagccacg
     9121 gcggaggcac ccagtccgcc gacgaacttg gtttgcacca gggccaccat cgagaacatc
     9181 aggttggcgg cgatcgaagg cagcgccagc tgcaggatgt gcgggcgttg cggaaccgga
     9241 gtggcagcgg ttgactcaac gctgctcata acactccgga ccgaccagaa gaggtcgggt
     9301 aggcgggatc agccgcaccg acggatcccg tcggtgcggc ctcgaggtgg cgcgatctac
     9361 tgctttttgc ccgtgaaagt gccgtcacca aacgagccaa gctttacttt gccatcggtg
     9421 atcttgtcgc cgtccagcgt accggtgtag accacttcca ggtcctgtcc ctgggcattg
     9481 atcttgtacg acagggtgat cacgttgcct ttgacgctcc cggtgactgg cgcttcaccg
     9541 agctggcctt tgtaggtgcc agtcacctga tcgcccttct ggaccagcgt gaaggtgggg
     9601 ctgccggtgc cagcctgcgt ttccaccgtc atcagccagt tgccggatac gtcggcaaaa
     9661 gccgcggtgg acatcaggaa accgaacgct gcaagcagcg gcgctaagaa aatgttacgg
     9721 atctttttca ttgtcactcc tccccagttg gacagcgggg atgggtaggt tacacagtct
     9781 gcagagcgct tgtcatttcg ttcgcggcgc tcatactgcg cgtccaggtt atttgctgtt
     9841 gcaggagaat gcagatgttg atgcgaactg cccgcgtcgc tggaatgggg atcgccctgc
     9901 tggctttggg aacgactggc actccggctg tcgcgcacca tgcctttcag gctgagttcg
     9961 atggcaatgc ccccgtcatg ctccagggca aggtcatgaa ggtggagtgg atcaatcctc
    10021 acacctgggt ccatatggaa gtcatcaccg acggacagcc gcaggagtgg atggtcgagg
    10081 ccggcacacc gaacacgctg ctgcgcgccg gcattaaccg cgacacgctc aagcctggcg
    10141 aagagatcat cgtgcgcggc tatcgcgcca aggacacccg ctgccgtccg gcctgcaaag
    10201 ccaatgggcg ggacgtgacc ttcaccgacg ggcgcaaggt gttcatgggc tcaagcggca
    10261 cgggcgcgcc gcgcgatgga tccgacccga acgagaagta acgaaagctc aacggatggg
    10321 ccagttagca gcctcgccta gcgcgacgct gctcccatca tctcctggaa gttcgtcgtg
    10381 tagaacttgt gcttatccgt ctccgaggtc tgttccagcg tccgctcgaa ccgaccgacc
    10441 ggatcgtcgg tgccttccgg atggggatag tcgctggaga acaggaacag gtccgctccg
    10501 gcgtccctga ccagacgacc gacgtcctct cccgggaacg gggtgaattt aatcgcgcgg
    10561 cggatatact cggatccctt caacgacagc gcagcgaccg acggatcggt ccgcttgaag
    10621 atcctctgac tcagatccag cgttcgcagg tactgcggta cccagcccgc accgagttcg
    10681 atgacccctc cccgcagctt cgggaatcgc tccagcacgc catcgaacac cagtgccgtc
    10741 agaaactgct gcggtccgat cgacaacagc atgaaatcac gcacccgcag attctcgccg
    10801 ccctccgcgc cggtaatgtc cttcggccgc ggctgaccgt tgttctcata ggctgaaagc
    10861 ttggcggaac tattcggccc gacgtgcagc atgaacggga catcaagatc gcagaatgcc
    10921 ttccagacgc cgtcgaagtc cggatggccg ggggatttgt caccgaccgg ctgacccggc
    10981 acccagaagg tcttgcagcc caggcgcacc ccctccttca gttcctctac gcagcgcgcc
    11041 ggatcggcca gtgaaagctg cccgacggcc agcagacgcg aatccttacc gcagaaagcg
    11101 gacattgccc ggttgtgagc gcgaattccg ccgtagcgca ggtccagatc ctcatgatgc
    11161 agatactgcg taccggaaaa agtcgaaaac acgagctgct tgcgaaagcc cagatcatcc
    11221 agtgccgcag cccgctcgcc ggcgtcgaac gcacctggcg cctgccaccc cttgggaccc
    11281 ttgaccacat cgtgagcctg cacgccggcg ctcgcacgat ccttctgtgc gttgatggcg
    11341 gctcgaatca catcgaaact cttggtaccc gacttcagca gactcatctc gggcagcctc
    11401 ggcagcagct ccggatcaac gaagctcgcg atccactccg gcgtctccat gatgtgactg
    11461 tcagcgtcgt agtagggacg gtcaaccgcg tacgtcatgt caaatcccca agcgcgccca
    11521 atccgtcggg gctattacac tcacgctata attaggtagc cgccaagtca agttgagccg
    11581 cgcgtcgcac cgcgatgtca cgatcagcgc tgactgtttc tagacgctgg cgcgccgccg
    11641 cgagcacggc ggccggcgcg cgctccggcc gcccgcagtc gaacggcggc tcaggagcat
    11701 actcaatccc gagctgcaag gcctgtgcag acgcggcgcc tgcgatctcg gctacgacgg
    11761 tcaacgccat gtcgattccg gcggtgacgc cgccgccggt gatggtccga ccgacgcgca
    11821 cgattcgagc gggatcgggc tgtacgccga attcggtcag cagatgacgc caagcccaat
    11881 gacatgccgc tcgcttgccg cgcagcagac ctgccgccgc cagcagtagc gatccggtgc
    11941 agaccgatgt gacatagcga gcgcagctcg ccagacgacg cagttgcgac aagtactccc
    12001 ggtcgtccag cgcctggacg gtgccgaagc cgccgggcac gcacaacagc gcacattgct
    12061 gcacgcttgc cagcggtacc acattggcaa aggccagtcc gccctcagcc tggatggtgc
    12121 cgccgccagt cgatgccaag gtgcaccgcg cccccggcag ccgcgagaag acttcatacg
    12181 gtcccgtgaa atccaactgc gtgattcgcg gatagagcgc gaacacgact tcaaatgact
    12241 ttgccatcac ccacctcctg atttgacggg atcatgttgt cgcaataccc tgtggctgga
    12301 atgacgttat tcccaccttt tgcgccatga ctcgaaacat cagcttcctg atctatccac
    12361 agttccagct gctggacgca gccggcccaa tttccgcatt cgaggtcgcg ggccgcatcc
    12421 gaccgggtgc ctatgcattg cgggtgatcg cgctgcagcc cggattggtg aagagctcgt
    12481 caggaatccc cctccccgcc atggctctgg gtgccgtaca tcgcgtggac accctgatcg
    12541 tgtccggagg cgatggttcg cgtgaggccg cgcaatgccc caggacacag cgattcattc
    12601 aggcctgcgc acgcagcgcg cgacgcacca gcagtgtctg ctcagggacc tacctgctgg
    12661 ccgcggccgg cctattggat ggcaagctcg ccaccaccca ctggagctgc accaccgact
    12721 tcaggtcgcg atttcccaag gtgcgccttg atccggatcg catctttgtg agggagggcc
    12781 gagtctggac ctccgccggc gtgactgcgg gcatcgattt ggccctggcg ttgatcgagc
    12841 aggacctggg cgaaaagatt gcgcgcgcga ccgcgcgcca actggtcgtc tatcatcgtc
    12901 ggccaggggg gcaatcgcaa ttctcggcgt tgctgcagat ggaatgttcc agcggaaaat
    12961 ttgcgccact gctggatgag gtgcgcagca gcctgcgtca ccgccactgt gttgccgagc
    13021 tggcagagcg agtctgcatg agtccacgcc agttctttcg cgcctttcag gccgacaccg
    13081 gcatgactcc ggccaaggcg gtggaacgcc tgcgtgccga tgcggcacgc gccgagctca
    13141 gcggcagcgc tcgctcggtg caggacgtcg cccgctcatg cggctttggc agcgccgagc
    13201 gaatgcgtcg cacatttctg cgcctgtatg gggtctcacc tattggcgtc aagcagcgcg
    13261 cgtattgact tcagtgggcc ggcccaacgt agatcgcgac gccgtttccg gtcttctgat
    13321 aggtaccgac ggaggtcccg aatcctaccc ccacattccc tgaaccgccg ccaaagccgg
    13381 agccgatgga gccggactgc cgatcgccaa taccctgcag caacacaccg ttggcgccga
    13441 gtttcgccgc ctcatccttc aggcgctgta tgaccttatc ggtcttgccc tgagcggtta
    13501 gcgcaaacga tccacggctg gaagtgttga gaatcgcgat ctgttcgtac tgtgccggcg
    13561 gattcgtata aatcgtgacc tgatccggcg aaataggggg tcggggcgct ccaaccagca
    13621 cgtgcgaact cgcacaggcc gctagcgcca cgactgcagt gatcgcggcc gccaccgagc
    13681 gcaccgatgg aactatcagc atcacagctc ctgtcgtgga cgttgaagtc agcttatcag
    13741 cagtcggagc ttcccgctcc ctcggaaacg ccgcgggtgc tcgcgacatc tggggcatcg
    13801 gcatcccggg cacgactggt ggtcaaacca tgatgcggcc agatcgatca gccgcgccgc
    13861 ttccgacacg agagcgatgt ggcgctcatc cagcgagtgg ccaagagcga ccgcattgcg
    13921 ttcgagtcgc tgatgcgccg ccacaatcgg cggcagtgca accgcgtggt cgcggcggtg
    13981 cttgctcggt ggtagttctt aagtccccgc tgaggtctac tcgacggcga cacccgcggt
    14041 actggcaatc ttcatgatct tgtccagatg agcctggacg gtcgggagca ttgtctgcgc
    14101 gaactggctg gcgtccttgt ctcgcccgga cgctatttcc ttcttgaaca gcgccaaggc
    14161 gtcacggtgc gactcgatct gatttttgat gtaggccttg tcgaaggaat cgccgctgag
    14221 cagtttcaag cgatcaccgg ctgccacctg ttccacgctg ggcgtggtcg gcatcttgac
    14281 gcctttgtgg gccgcggtag cccatagttt ctggtccgca gtcgagtgat ccttgaccat
    14341 catgccgccg aaatccctga gcgcggcgct gctggccttg gcctcggcca gtttggcgac
    14401 tgccacctcc gccagtccac cttcaccggc gctcttgtag aacgactcgt cgggattcga
    14461 atcggcaccc agagccagca gcggcacgat ccctatcaac catcctatga atgcccgttt
    14521 catacgtaac tccgcaacac cgcaaaggcg gcaaactgtg catcgcatcg tggacaagct
    14581 ggcagcattg cgggaccggt tggccaagcc gcaaaacccg cgcctttagc gccatttcgg
    14641 gtaggaagat tccctcgcgt cagcagcagt atgtcatgcc gcgtcgtgaa agatcacgcc
    14701 gagcgtgaag cgcgctcccg acagcagctt actcacgcca tggcgctgat tgacccgata
    14761 gtcaccgcgg ctgcctcgaa ccgggcgctg ccgaacagcg aataccagtg catcgccctg
    14821 tttcagtgga gcgaccgtcg ctcgcgtctg catgcgcggc cgctgctccg tgagaacgaa
    14881 ctcccctccg gtgaagtcgc gccccggctc agacagcagg atcgccaact gcagtggaaa
    14941 cacatgctcg ccatacagat cctggtgcag acagttgtag tcaccttcac cgtacttgag
    15001 cagcagtggc gttggcagtg tctggcccgc ccgatggcag cgatccagaa actcgacatg
    15061 acaatacggg aagcgcacat cgagcttcat gcgagcattc caggcgttgg ccagcgccgt
    15121 gagatgcgga tagatctcgg tgcgcaactg ttgaatgacg ggcaataacg gataacggaa
    15181 gtacttgtat tcaccgcgtc cgaatccgtg acgtgtcatg atgacgcgac tgcggaaata
    15241 gcgctcctcg tcgtacagtg cggcaatgtc gtcgcacttg ggcggtgcaa gcagttgctt
    15301 cagtaccgcc caaccctgga catcgagcgt gccagacagc gccgaccagt ggctatcgtt
    15361 cggcgtgctc acgggcggct cacgccctcg cgcgtcaaca gtgtccgttt gcgctcgatc
    15421 ccccaacgat aacccgatag cgccccgtcg gtacggacta cccgatgaca aggaatggcg
    15481 atcgccagcg gattggcgcc gcaggcctgc gcgacggcgc gcacggactg gggtgcaccg
    15541 atcttttttg cgatgctggc atagctgacg gtggatcccg ccgggatctt gctcagcgct
    15601 ttccagaccc ggcgctgaaa tgccgttccg cgaacatcga gcggcagatt gctgcccagg
    15661 ctgggtgcct cgaccagtcc gacgacctgc gccaccagcg actcgaatcc ctcatcggac
    15721 cctaccagag tcgcgttgga gaaacgatcc tgcagttcac gcaccaactg attgggatcg
    15781 tctcccatcg tgatcgcgca cacgcccttg gcgctgaccg ccaccagaat ggcaccgagc
    15841 gaactctggc ccaccgcgaa tcggatctgc tcatcggcac cgcgtcggcg aaactgactg
    15901 ggcgacatcc ccaacatggc gttggcctgc tcgtagaagc gactgttgga gttgaagccc
    15961 gcatcgaaga tcgcttccgt cacgctcgat cccgtctgca gcgagcgttg cactcgccgc
    16021 gtgcgatgcg cgttcgcgta ctccttaggg gtcactcccg tgaccgcttt gaacagccgg
    16081 tgaaagtgat aggggctgat accactgtct ttcgccagca ctgccaggct cggcgcctct
    16141 tcggcggatt cgatcatccg gcaggcacgc gccacggtgg ccgcatgctg cacctcgagc
    16201 ggcggtcgat cggggcggca gcgcttgcag gcgcgaaatc cagccgcctc ggccgccgcc
    16261 gtggtcgcgt agaaggcgat attccccggg ttggcccgtc tggaagggca ggagggtcgg
    16321 cagtacacgc cggtcgtttt gacagcataa aagaacttgc cgtcctggcg gcggtcgcgg
    16381 gccaccaccg cggaccagcg cggatcgttg agggtcttga tggcggcagc ctgccgcgtg
    16441 atctgagctt gcattggagg catctcctgt ttcagcctag gtgccagtct gaccaccgtc
    16501 actcccgcca ccactccggg tcttgcgatt gaattgaatg cccgaacaga tccgctttca
    16561 caggtttcca gtcatgggga tcgaggcgat gagtgcaagc acgtcgcacg tatttccccg
    16621 gcatacgcac gaccggtatg gtatcggagt cgttgacgcc ggtggacacg cctcgctcag
    16681 cgaccgcggc caggtcgagg ccggaccggg tggcctgatc tgcgtcaatc cgggcgaggt
    16741 tcacgacgga cgtccgagtg gtggcgtgtc acgatcctgg cgaatcgtct atctcgagcc
    16801 gacgctgatg cgcggccggt gccgggaggt ctctgagggc cgttcggcga cgctgtattt
    16861 tgccgcgccg gtctttgcgg acgaaacgct gcgagcgctg ttcaaccaga cctttgtcag
    16921 caccgaatcg atggcatgcg aaagcggact gctgatgatc gtcgcgcgaa tgcgcgcccg
    16981 atggaccgtc gaccgcgcgc cgtccaggac cacaggatgc atccgccgcg cacgcgaacg
    17041 catcgacagc aatcccgcag agccacatac actcagcgat cttgcgaacg agggcggcct
    17101 gagtcgctat cagctgttgc gcagtttcgc gcgcgagtta cggctgacgc cgcacgccta
    17161 cctgctgcaa cagcgtatcg cgctggcgca ccggctgatc cgacaagggg gacggccgca
    17221 ggccgagata gccatcgcga gcgggttctt cgatcagagc cacttcagcc gctgcttcgc
    17281 acggcagttc ggagtgagcc cgcgacgcta tgccgcgcag cgcgcttaaa gccactgcaa
    17341 ttttttacaa gatcgcatcg ccgcagctga agcacactgt tcgccatgca cccgagcgcc
    17401 agcatccgcc gctgccgtga cgaggagcgc gtcatcatcc tgcagatctg caatcaggcc
    17461 gctgaagcct atcgccatac gattccgcgc gattgctgga aggaaccgta catgccccag
    17521 gacgaacttg atcaggaaat cgccaacggg gtgcgcttct ggggctacga gtctgaacag
    17581 ggactgtcag gt
//
LOCUS       DATMRM010000219         3035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4157786, whole genome shotgun sequence.
ACCESSION   DATMRM010000219 DATMRM010000000
VERSION     DATMRM010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4157786"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VNZ06_06475"
     CDS             complement(<1..252)
                     /locus_tag="VNZ06_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20432.1"
                     /translation="MQQPADILFVLPLRYEDRTQVSRIGALQAGVSAVVEGEVLLSEI
                     VYRRRRQLLVRVSDGSGALTLRFFYFSRAQQEGLARGTRV"
     gene            complement(328..708)
                     /locus_tag="VNZ06_06480"
     CDS             complement(328..708)
                     /locus_tag="VNZ06_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016803893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWW20433.1"
                     /translation="MQRQIIQTPDAPKAIGTYSQAVKVGNTVYISGQIPLDPATGQLV
                     SGDIEAQIRRSFENLKAIAQAAGGSLADAVKVTVFLTDLAHFAKVNEIMATYIHEPYP
                     ARAAVGVASLPRGARVEVECILSL"
     gene            complement(843..2966)
                     /locus_tag="VNZ06_06485"
                     /pseudo
     CDS             complement(843..2966)
                     /locus_tag="VNZ06_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195510.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
BASE COUNT          537 a          965 c          952 g          581 t
ORIGIN      
        1 cacgcgcgta ccgcgcgcca gcccttcctg ctgggcgcgc gaaaagtaga aaaaccgcag
       61 tgtcagcgcg cccgagccat cgctgacgcg caccagcagt tgccgtctgc ggcgatagac
      121 gatctccgac agcagcacct ccccttcgac caccgccgac acacccgcct gcagtgcacc
      181 gatgcggctg acctgggtgc ggtcttcgta gcgcagcggt agcacgaaca ggatgtccgc
      241 tggctgctgc atgggggctg cgcgcgatgg cgccggtgca atcgctgacg gcttggtcat
      301 gtcttgccgg gcgcggtggg ttctacgcta gaggctcagg atgcattcca cctcaacgcg
      361 cgcgccgcgt ggcagcgatg ccacgccgac ggccgcgcgc gccggatagg gttcatgaat
      421 atacgtcgcc atgatttcgt tgaccttggc gaaatgggcc aggtcggtca gaaacacggt
      481 gaccttgacc gcatccgcca gcgaaccacc ggccgcctgt gcgatcgctt tgagattctc
      541 gaacgagcgg cggatctgcg cttcaatgtc gccggacacc agttggccgg tggccggatc
      601 gagcgggatc tggccggaga tataaacggt gttgccgact ttgaccgctt gcgaatacgt
      661 gccgattgct ttgggggcgt ccggggtctg aatgatctgt cgttgcatga gtgcttgggc
      721 tctcgtgggt ggaagacgat agtatggccg gcaaaggccg ccggccgcgc tcaattatcc
      781 gcgctctggc tgtcctcgcc acgctcgtca ggctcatcag gctcgtgacc gtgaccgtgc
      841 gtcgcgatcg tacggatgac acgcagtacg tcgggcatcc gacgcaccac gcgcatcacg
      901 cgggccaggt gcttgcgatc agtgatctcg agcacgaaca cgatcactga catctcggca
      961 tcgcgctgct cgatcgagac cgtgctgacg ttggtttgcg tgccggcgat agcggccgag
     1021 acggcagcca gaacgcccat gcgggtcacc gtctcgatgc ggatctccga cagaaacaga
     1081 ctcccgggtt tgtgctgcca gcttactggc agccactttt cggggtgctt gtggtagtcc
     1141 tcgacgttgg cgcaggtatc gcgatgcatc acgatgccac gcccggtgct caagaaggcc
     1201 aggatcggat cgttcggtat cgggtagcaa cagtgcgcat agctgacgag caagccctcg
     1261 gtgccggcga ccgccagcgg catcggcgcc ccgttgctca cgtcagcacg ctcagtgggc
     1321 agcagccggc gtgcgaccag cggcgccagg cgctctccga gtcctacttt ctcgtacagt
     1381 tcgtccggat ccttgaggcc gaactcaccc agcgccgact gcaacgccgc cggttccacg
     1441 gcgtccagct tcacgtcgaa ctcggccagc gcctgaacca acagccgtcg tcccagctcg
     1501 accgcctcat tgcggcgtag ccccttgagg tagtggcgga tggccgaacg cgctttggca
     1561 gtcacgacga aattcaccca cgaaggattc ggagttgcgc ccttcgcggt gatgatctga
     1621 acggtctgac cattgcgcag taccgtgcgc agcggcgcca ggcgccggtc gatcttggcc
     1681 gctacgcagc gattgccgat atcggtgtga accgcatacg cgaaatcgac caccgtggcg
     1741 ccacgcggca gacgcaggat ctcaccgcgc ggcgtgaaga cgtagacctt gtcggggaac
     1801 agatcaacct tgacgctctc gatgaactcc tccgagttgc ctccttcctc catctccacc
     1861 aggttggaaa gccatttgcg cgcccgctcc tgttgggcgg agccttccgg ctctccaccc
     1921 ttgtatttcc aatgggcggc gatgcccgat tcggccacgc gatccatgtc ctcggtacgg
     1981 atctggactt cgatcggcac accgtttgga ccgaacagcg tcgtgtgcag cgattggtag
     2041 ccattcacgc gcggtatcgc aatgtagtcc ttgaagcgac cgggcatcgg cttatagaca
     2101 ctgtgtacga tgccgagcgt gcgatagcag gtgtccactg aatcgaccgt gatccgcagc
     2161 ccgtagacat cgacgatctc ggccagcgtg gcgcgcttgc gcagcatctt gcgatagatg
     2221 ctgtagagat gcttctcgcg cgattcgaca tcgcctgcaa cagtggattt cttcagggcc
     2281 gtttccagct gcgcgcggat tttgttgaga aactccttct gattgccgcg cgcgcgcttg
     2341 agtgcacgct cgatcacgcg ataacgctgc gggtagagcg tacgaaagcc gagatcctcg
     2401 agctcgagct tcatcgaata cagcccgagc cgctcggcaa ccggcgcgta gatgtcgagt
     2461 gtctcgcggg cgatggcgcg gcgcttggcc ggggccagcg catcgatggt gcgaagattg
     2521 tgtgtgcggt cggcgagctt gaccagaatc acgcgcaggt cgcgcaccat cgccagcatc
     2581 atcttgcgga agttctccgc ctgcgcttcc tcgcgattct tgaagcggat ctgatcgagc
     2641 ttggtcacgc catcgacgat ctcggcgacg tcgttgccga agcgctgcgc aatgtcagcc
     2701 ttcgcgatcg gcgtatcctc gatgacgtcg tgcaggatcg cgccaacgat ggtgtcggca
     2761 tccagatgca ggtcagccag aatttcggcg gccgcaaccg gatgcgtgat gtacggttcg
     2821 cccgagacgc gcttctggcc ctgatgcgcc tcggcaccga attcggtcgc ttcgcggatg
     2881 cgctgcacct gctccgcggg cagataggtc gagaccttgg ctaaaagctc cttgaccccg
     2941 ggcgtgccgc gccggacgcc aggcaggaaa cccagtatcg atgacgcata gtccacagcg
     3001 aacccggacc cgcgccgtag agcttagagc tcgcg
//
LOCUS       DATMRM010000220         7635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_751946, whole genome shotgun sequence.
ACCESSION   DATMRM010000220 DATMRM010000000
VERSION     DATMRM010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751946"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..484)
                     /locus_tag="VNZ06_06490"
     CDS             complement(65..484)
                     /locus_tag="VNZ06_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20434.1"
                     /translation="MSADLLEPQVRRLFSSLTHAGDGKSDSDEEHAGEWRLVEGEAGH
                     REHGTRVRFILRLRGSQLVGARYYAYGCPHTLAVCEWLAERLEAGEMAALGAPADWCA
                     SLGVPIVKLGRLLVVEDALRSALGKAVLVATETAKTL"
     gene            complement(481..1641)
                     /locus_tag="VNZ06_06495"
     CDS             complement(481..1641)
                     /locus_tag="VNZ06_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWW20435.1"
                     /translation="MSESAPIYLDCAATSPVDPRVAEAMLGCLTREGVFGNPASLHPF
                     GQIARARVERARAQVAALIHAPSPQVVWTSGATESNNLAILGTARAMAKRGRHLISVR
                     TEHKSVLDPCKRLEQEGFAVSFLDTDRDGLVNPHQLDQLLRDDTVLVSVMQANNEIGV
                     LQDIEAIGAICQARGVPLHVDAAQSAGKIDIDVRNLKCDLLSFTAHKLYGPKGVGALY
                     FSPARRASLQPLIFGGGHERGLRSGTLPVHQIVGFGVACEMAASSRVADAAHCERLRT
                     RLWNGLSRIEGVLLNGHPARRVASILNVAFPGIEGESLLMGLTELAVSTGSACNSESD
                     EPSYVLRALGRDTELAQSSLRFSVGRFSTEAEVDTAIAAVTREVKRLRAAAA"
     gene            complement(1634..2383)
                     /locus_tag="VNZ06_06500"
     CDS             complement(1634..2383)
                     /locus_tag="VNZ06_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWW20436.1"
                     /translation="MPIPIRIVLVETSHPGNIGAAARALKNMALEQLVLVNPKHFPHP
                     EASARASGAEDVLNAARVTPDLPTALAGCGLVLATTSRERDQNFRVLDPHAAAHRAVD
                     HAARGPVAIMFGSERTGLTNDELRYAHALLAIPANPAYPSLNVAMAVQIVAYEIMCAV
                     LERSQEAPRSITTPAVPLADPVEMDRLYAHLAEVLDEIDFRDHTQSGIHLMKRIRRLF
                     QRAEPDLNEVHILRGILTAVQQRRRRAVPHE"
     gene            2405..3205
                     /locus_tag="VNZ06_06505"
     CDS             2405..3205
                     /locus_tag="VNZ06_06505"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWW20437.1"
                     /translation="MHPLLNIGVRAARRAGEVIVRALVRLESLEVTSKGRNDYVSEID
                     RAAEGEIIDVIHKHYPEHAILAEESGRSGENDTVWIIDPLDGTTNFLHGFPVFAVSIA
                     VQQRGRLEAAVIYDPMRQEIFTASRGGGAHLENHRMRVSKQRGLEGALLSTGFPYRAD
                     EPYADNYFAMFRTLTMLTAGIRRPGSASLDLAYVAAGRTDGFWELGLKAWDTAAGTLM
                     VVEAGGRVGTLAGDEYQLGPNIVAGSPRVFEGIVAAIAPHVPEALRNV"
     gene            3242..4744
                     /locus_tag="VNZ06_06510"
     CDS             3242..4744
                     /locus_tag="VNZ06_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWW20438.1"
                     /translation="MNHAVGTSVGLFATKPIADYHAEAAGKHLIRALGIPALIAFGIG
                     CIIGTGIFVLTGLAAAQHAGPAIGISFIIAGLGCMFAGLCYSEFAAMVPVAGSAYSYS
                     YATLGEFVAWFVGWNLVLEYMMACATVSVGWSRYLVKLLEHFNINFLPSALTSAPLRA
                     VGDGFNVERTGAIINLPAVVIITIAAALCYKGIKESARVNNLIVAIKVSIVLAVIAFG
                     ASYINSANWIPFIPPNTGTFGQFGWSGIIRAAGVIFFAYIGFDAVSTVAQEAKDPTRG
                     MPIGMLGSLLICTVLYILMSSAMTGMVPFQQLDDAAPVIVALNAHPELKWLQVWVLVG
                     ALCGLTSVIITMIIPQARIWLTMSRDGLLPKFFGAVHPRFHTPHKATVITGMAAATFG
                     GLLPIDILGELVSIGTLIAFIVVCIGVLVLRYTRPDLHRPFRVKGIWVVSILGVFFCA
                     GMALFLPSGTWIRLLVWSLIGFALYFVYGYRHSRLRSGTAPVTDASQRQA"
     gene            complement(4741..5448)
                     /locus_tag="VNZ06_06515"
     CDS             complement(4741..5448)
                     /locus_tag="VNZ06_06515"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HWW20439.1"
                     /translation="MLTALESPQNWIALAILTLLETVLGIDNVIFLSILVERLPAARQ
                     RSARTVGLMLAMLTRLLLLSCVVWLSRLTAPLFSAFDQCFSGRDLILGAGGLFLLAKS
                     ALEIHSTVGAHQAPRAKRVLDNYVAVVGQIAVLDMVFSLDSVFTAVGMARPEQAPIMA
                     AAIVISILTMLWLAGSVAGFIRRYPSMKVLALAFLVLIGAALLAEAFEFEVPKGYLYF
                     AMAFAFAVELINIRLRR"
     gene            complement(5454..6386)
                     /gene="secF"
                     /locus_tag="VNZ06_06520"
     CDS             complement(5454..6386)
                     /gene="secF"
                     /locus_tag="VNZ06_06520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HWW20440.1"
                     /translation="MEFFHRATNFPFMSTRRVWYALSALMILVSLGSLLLRGLNLGVD
                     FTGGVTVQASFPDNANRDAVDTALKRAGYAEPQVTIFGRASDMAIRLPPTKLSTEAVR
                     TQLGQILHGVDAKVQIQQVDVIYPQVGGELLRSAWQGMIATLLAIGAYIILRFHTWRL
                     SFGAILAVLHDPILVFGVFSLTHTPFDLTVVAAMLAVIGYSLNDTVVVFDRIRERFHV
                     ARRLDPVMVLDQSINQTLSRTIMTKVVTLIVVVALYALGGPVLRGFSEALIIGILAGT
                     YSSIYISSAVALDCGLKAEHVLPPPPRRQADDTP"
     gene            complement(6412..>7635)
                     /gene="secD"
                     /locus_tag="VNZ06_06525"
     CDS             complement(6412..>7635)
                     /gene="secD"
                     /locus_tag="VNZ06_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HWW20441.1"
                     /translation="VRMVLTPAQIKARQDYALEQNRTTLSNRVNELGVSEPIVQRQGL
                     SRINVQLPGVQNSAEVKDLLGKVATLEFRLEDTQNNAAQAEQSGHAPLGTKLYTYQGR
                     PVLLKREVIVTGDELTSATSSAGQEGPEVSIKLDARGAESMFRTTRLNVGKPMAVVFI
                     EKSRRQIEVNGQKIDKDTTSEKVINIATIQGVFSSQFRITGLGSGEARDLSLLLRSGS
                     LAAPVEVVAESTIGPSLGEDNIKKGVLALEIGMLAVFVFMALYYHAFGLVADAVLVAN
                     VILLTALLSMMHASLSLPGIAGIILTVGIAVDANVLIYERIREELRNGVSPQAAIRAG
                     FDKAFSAIADSNVTALIAGLALWVIGTGAIRGFAVVLTLGIATSMFTSLLGSRALVTL
                     IYGGGRRKIESLSIG"
BASE COUNT         1417 a         2497 c         2347 g         1374 t
ORIGIN      
        1 gacccgttcc gccgcagatt ccgtcaaaga aatggccatc gcaacattct ccaattaatt
       61 cattttacaa cgttttcgcc gtctccgtcg cgaccagtac cgcctttccg agcgccgagc
      121 gcaatgcatc ttcgactacc agcaggcgac ccagtttcac tatggggact cccaagctcg
      181 cgcaccagtc cgcgggggct ccgagcgcgg ccatttcccc cgcctccagc cgctcggcca
      241 gccattcgca gaccgccagt gtgtgcgggc agccgtaggc gtagtaacgg gcaccgacca
      301 gctgcgatcc gcgcagacgc aggatgaagc gcacacgggt cccgtgctcc cgatgcccgg
      361 cctcgccctc gaccagccgc cactcgccgg cgtgctcctc gtccgaatcg gatttcccgt
      421 ctccggcatg cgtcaggctc gagaacaggc gccgaacctg cggctcgagc agatcggcac
      481 tcatgctgcc gccgctcgca gtcgtttcac ctcgcgcgtg acggcagcga tggccgtatc
      541 aacctccgcc tcggtactga agcgcccgac gctgaagcgc agcgagcttt gcgccagttc
      601 ggtgtcgcgc ccgagcgcac gcagcacata ggaaggctca tcactttcgg aattgcaggc
      661 cgatccggtc gagaccgcca gctcggtcag acccatcagc agactctcac cctcgatacc
      721 ggggaaggcc acgttgagga tcgaggcgac tcgacgggcc ggatgaccat tcagcagtac
      781 cccctcaatg cgcgacaggc cgttccacag tcgtgtccgc agacgctcgc aatgcgccgc
      841 atcggccacc cgactggagg cggccatctc gcaggctacc ccgaagccca cgatctgatg
      901 cacgggcaga gtccccgaac gcagaccccg ttcgtgaccg ccaccgaaga tcagcggctg
      961 cagcgacgcg cgacgcgccg gggaaaaata cagcgcgccc acgcccttgg gaccgtagag
     1021 cttgtgcgcc gtgaacgaca gcaggtcgca tttcaaattc ctgacgtcaa tgtcgatctt
     1081 gcccgcgctt tgcgcggcat ccacatgcag cggcacaccc cgtgcctggc agatcgcgcc
     1141 gatcgcctcg atgtcctgca ggacgccgat ctcattgttg gcttgcatga ccgacaccag
     1201 cacggtgtca tcccgcagta actggtcgag ctgatgagga ttcaccagac cgtcacgatc
     1261 ggtgtccaga aagctcactg cgaagccctc ctgctcgagc cgcttgcacg gatcgagcac
     1321 cgatttatgc tcggtacgca cgctgatgag atgtcggccg cgcttggcca ttgcgcgcgc
     1381 ggtacccaga atcgccaggt tgttggattc ggtagcgccg gaggtccaca cgacctgggg
     1441 tgagggtgcg tgaatcaatg cggcaacctg ggctcgtgcc cgctcgacgc gcgctcgggc
     1501 gatctgccca aaaggatgca gcgatgccgg attgccgaac acgccttcgc gcgtcaggca
     1561 cccgagcatc gcctcggcga cccggggatc caccggagag gtcgcggcgc agtccagata
     1621 gatcggggcg gactcactca tggggtaccg cccggcgccg gcgctgctga accgcggtca
     1681 gtatgccgcg caggatgtgc acttcgttca gatcaggctc cgcgcgctgg aacaggcgac
     1741 gtatccgctt catcaggtga attccgctct gcgtgtggtc ccggaagtcg atctcatcca
     1801 ggacttcggc cagatgcgca tagagacgat ccatctccac aggatccgcc agcggcacgg
     1861 ctggcgtcgt gatgctgcgc ggcgcctcct gggagcgctc cagcaccgcg cacatgatct
     1921 cataggccac gatctgcacc gccatcgcca cattcagcga cggataggcg gggttggcgg
     1981 gaatcgccag caaagcgtgc gcataacgca gttcatcgtt ggtcagaccg gtgcgctcgg
     2041 agccgaacat gatcgccacc ggcccacggg cggcatgatc gaccgctctg tgcgccgcgg
     2101 cgtgcgggtc gagcacacgg aagttctgat cgcgctcgcg gctggtggtt gccagcacca
     2161 gcccgcaacc ggcgagcgcc gtgggcagat cgggtgtcac ccgcgcggcg ttgagcacat
     2221 cctcggcacc ggatgcgcgg gcactggctt cgggatgggg aaagtgcttc gggttcacca
     2281 gtaccagttg ctcgagcgcc atgttcttca gcgcgcgcgc ggccgctccg atattgcccg
     2341 gatgcgaggt ctcgaccagc acgatgcgaa ttgggatcgg cactactggt atcctaaact
     2401 tcccatgcat ccactgctca acatcggcgt acgcgccgct cgccgtgccg gcgaagtcat
     2461 cgtccgcgcc ctggtgcggc tcgagtcgct ggaagtcaca tcgaagggcc gcaacgacta
     2521 cgtgagtgag atcgaccgcg ctgccgaagg cgagatcatc gatgtgatcc acaagcacta
     2581 tcccgaacat gcgatcctcg ccgaggagag cggccgcagc ggcgaaaacg acaccgtctg
     2641 gatcattgat ccgctggatg gcaccactaa tttcctgcac ggttttccgg tgttcgcggt
     2701 ctcgatcgcg gtccagcagc gcgggcgact cgaggccgca gtgatctacg atccgatgcg
     2761 ccaggagatc ttcaccgcgt cgcgcggcgg tggcgcgcat ctggagaatc atcgcatgcg
     2821 cgtcagcaaa cagcgtgggc tggaaggcgc gctgctgtcc accggctttc cctatcgcgc
     2881 tgacgagccc tacgcggaca attactttgc catgttccgc acgcttacca tgctgaccgc
     2941 cggtattcgt cgtccgggct ccgcatcgct cgatctggct tatgtcgccg ccgggcgcac
     3001 cgatggtttc tgggagttag gattgaaggc ttgggatacc gcggccggga cgctcatggt
     3061 cgtggaggcc ggcggccgtg tcggaacact ggcgggcgac gagtatcagc tcgggccaaa
     3121 tattgtcgcc ggctcgccca gagtattcga gggaatcgtg gcggccattg cgccgcatgt
     3181 accggaggct ctgcgcaacg tgtagagtgc ggccacgaca ggcaagacag cgggaattcc
     3241 aatgaatcat gccgtaggga ctagcgtcgg actgttcgcg accaagccca ttgccgacta
     3301 tcacgccgaa gcggcgggca agcacctgat ccgcgcgttg ggtatcccgg cactgatcgc
     3361 cttcggcatc ggctgcatca tcggcactgg catcttcgtt ctcaccggac tggctgccgc
     3421 gcagcacgcc ggacctgcga tcggcatttc cttcatcatc gccggactcg gctgcatgtt
     3481 tgcgggactg tgctactccg aattcgccgc catggttccc gttgccggca gcgcctattc
     3541 gtacagctat gcgacgctgg gcgagttcgt tgcctggttc gtcggctgga acctggtgct
     3601 cgagtacatg atggcctgtg ccacggtatc agtcgggtgg agccgctacc tggtcaaact
     3661 gctggaacat ttcaatatca atttcctgcc gagcgcgctg accagcgctc cactgcgagc
     3721 cgtcggcgac ggcttcaacg tcgagcgaac cggtgccatc atcaatctgc cggcagtcgt
     3781 catcatcacg attgcggccg cgctttgtta taagggcatc aaggaatccg cgcgcgtcaa
     3841 caatctgatc gtggccatca aggtcagcat cgtgttggcg gtgattgcgt ttggcgcctc
     3901 gtacatcaac agcgccaact ggataccctt cattccgccc aacactggca catttggcca
     3961 gtttggctgg tctggaatca tccgggccgc cggtgtgatc ttctttgcct acatcggttt
     4021 tgacgcggta tccaccgttg cacaggaagc caaggatccg acgcgcggca tgccgatcgg
     4081 catgctcggc agtctgctca tctgcaccgt gctctacatt ctgatgtcca gtgcgatgac
     4141 cggcatggtg ccgtttcagc aactcgacga cgcggccccg gtgatcgtcg cactgaacgc
     4201 acaccccgag cttaagtggc tgcaggtgtg ggtgctcgtc ggcgcgctgt gcggcctgac
     4261 ctcggtgatc atcacgatga tcattccaca ggcccgcatc tggctgacga tgtcgcgcga
     4321 tgggctgctg ccgaagttct ttggcgcggt acaccccaga ttccacacgc cccataaggc
     4381 gaccgtgatc accggcatgg cggccgcaac ctttggcgga ttgctgccga tcgatatcct
     4441 gggcgagctg gtatcgatcg gcacgctgat cgcctttatc gtggtgtgca tcggcgtgct
     4501 ggtgttgcgc tacacacgcc ccgacctgca ccgcccgttc cgcgtcaagg gcatctgggt
     4561 ggtatcgatc ttgggcgtat tcttctgcgc tggtatggcg ctattcctcc cgagtggcac
     4621 ctggattaga ttgctggtgt ggagcctcat cggcttcgcg ctgtacttcg tctacggcta
     4681 caggcacagc cgactgcgca gtggcaccgc accggtgacc gacgccagcc aacggcaggc
     4741 ttagcgccgc aggcggatgt tgatgagctc gaccgcgaag gcgaacgcca tcgcgaagta
     4801 cagatagccc ttcggcactt cgaactcgaa tgcttcggcc agcagcgccg cgccaatcaa
     4861 caccagaaag gcgagcgcca ggaccttcat gctcggatag cggcggatga acccggccac
     4921 cgagcctgcc agccacagca tggtcaggat cgagatcacg atggcagcgg ccatgatcgg
     4981 ggcctgttcg ggcctggcca tgcccacggc ggtgaacacc gagtccagcg agaacaccat
     5041 gtcgagcacg gcaatctgcc ccaccaccgc gacgtagttg tcgagcacgc gcttcgcgcg
     5101 cggcgcctga tgcgccccga ccgtgctgtg aatctcgagc gcactcttcg caagcaggaa
     5161 cagtccgccg gcgcccagga tcagatcgcg ccccgagaag cactgatcga acgcgctgaa
     5221 cagcggcgcg gtcaggcgcg acagccacac cacgcacgac agcagcagca gccgggtcag
     5281 catcgccagc atcagaccga ccgtgcgggc gctgcgctgc cgggcagccg gaagccgttc
     5341 caccagaatc gacaggaaaa tgacgttgtc gatcccgagc accgtttcca gcagcgtcag
     5401 gatcgccagc gcgatccagt tctgcggaga ctcgagcgca gtgagcacgt gccctagggc
     5461 gtatcgtcag cctgtcggcg gggcggtggt ggcaggacgt gctcggcttt cagaccacaa
     5521 tcgagcgcga ccgcgctgga aatgtagatc gaggagtagg tgccggcgag gatgccgatg
     5581 atcagggcct ccgagaagcc ccgcagtacc ggaccgccca gtgcgtacag cgccaccacc
     5641 acgatcagcg tgacgacctt ggtcatgatg gttcgtgaca gcgtctgatt gatcgactga
     5701 tcgagcacca tcaccggatc gaggcgccgt gcgacgtgaa agcgctcgcg gatgcgatcg
     5761 aacacgacca cggtgtcgtt gagcgaatag ccgatcaccg ccaacatcgc cgccaccacc
     5821 gtcagatcaa acggcgtatg agtcagagaa aacacgccga acaccaggat cgggtcgtgc
     5881 agcaccgcca ggatggcacc gaacgacagg cgccaggtat gaaaccgcag gatgatgtaa
     5941 gccccgatcg ccagcagcgt cgcaatcatg ccctgccaag cgctgcgcag cagttcacca
     6001 ccgacctgag ggtagatcac gtcgacctgc tgaatctgta ccttcgcatc gacgccgtgc
     6061 aggatctgcc cgagctgcgt gcgcaccgcc tcggtgctca gcttggtggg tggcagcctg
     6121 atcgccatgt cgctcgcgcg gccgaaaatg gtgacctgcg gctcggcgta gcccgcgcgc
     6181 ttcagcgcag tgtccacggc atcgcgattg gcattgtcgg ggaagctcgc ctgtaccgtg
     6241 acgccaccgg tgaagtccac gcccagattc aggccgcgca gcagcagtga gccgagcgac
     6301 accagaatca tcagcgccga cagcgcgtac cacaccctgc gggtggacat gaaggggaag
     6361 ttggtcgcgc gatgaaaaaa ttccaaggtc tttctctttg tgaatcgttg atcagccgat
     6421 cgacagactt tcgatcttgc ggcggccacc gccatagatc agcgtcacca gcgcgcggct
     6481 gcccagcagc gacgtgaaca tcgaggtggc gatgccgagc gtcagcacca ccgcaaaccc
     6541 acggatcgcc ccggtaccga tcacccacag ggcgagaccg gcgatcagag ccgtcacgtt
     6601 cgagtccgcg atcgccgaga aggctttgtc gaatccggca cggatcgcgg cctgcggcga
     6661 gacgccgttg cgcagttcct cgcgaatacg ctcatagatg aggacgttcg cgtccaccgc
     6721 gatgccgacg gtgagaatga tgccggcgat tccgggcagc gacagcgagg catgcatcat
     6781 cgacagcagt gccgtcagca ggatcacgtt ggcgaccagc accgcatcgg ccaccaggcc
     6841 gaacgcgtgg tagtagagcg ccatgaagac gaacaccgcg agcatgccga tctccagcgc
     6901 cagcacgccc tttttgatgt tgtcctcgcc caggctcggt ccgatcgtgc tttcggcgac
     6961 cacttctacc ggcgcggcca gtgaacccga gcgcagcagc agcgacaggt cgcgcgcttc
     7021 gccggaccct aaacccgtga tccggaactg cgaagagaac acgccctgga tcgtcgcgat
     7081 gttgatcacc ttctcgctgg tggtgtcctt atcgatcttc tgtccgttga cttcgatctg
     7141 ccggcggctc ttttcgatga acaccaccgc catcggcttg ccgacgttca ggcgggtggt
     7201 gcgaaacatc gattccgcgc cacgtgcatc cagtttgatc gacacctccg ggccttcctg
     7261 gccggccgaa gaggtggcgc tggtgagctc atcgccggtc acgatgactt cgcgtttcaa
     7321 aaggaccggg cggccctgat aggtgtagag cttggtgccc agcggggcat gtccgctctg
     7381 ctccgcctgg gcggcgttgt tctgcgtatc ctccagacga aactccagcg tggcaacctt
     7441 tccgagcagg tccttcacct cggccgagtt ctgcacgccc ggcaactgga cgttgatgcg
     7501 actgagtccc tggcgctgca cgatcggttc ggagacgccc agctcgttca cacggttact
     7561 gagggtggtg cggttctgct ccagtgcata gtcctggcga gccttgatct gcgccggcgt
     7621 cagcaccatg cgcac
//
LOCUS       DATMRM010000221         2420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2293857, whole genome shotgun sequence.
ACCESSION   DATMRM010000221 DATMRM010000000
VERSION     DATMRM010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2293857"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VNZ06_06530"
     CDS             <1..661
                     /locus_tag="VNZ06_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWW20442.1"
                     /translation="AVKRRLGRKRSNVQRTSEMRARLMQAAIDTLYRAGYSATTTIEV
                     ARRARVSRGAMLHHFPTRTHLLLATAHHIVESDRLFRREKFFPIRLSPGLERFYAASD
                     VSWELHRRPSSIALLEIMMATRSDKALAKAFAPFQKMWEQARHAAAARMAADLGVEDL
                     VAVTNLIRLHQACMRGLAIELMFTHHAQEVEAARQLQVGFDRAFAQNLVAEARKRGSR
                     A"
     gene            658..1242
                     /locus_tag="VNZ06_06535"
     CDS             658..1242
                     /locus_tag="VNZ06_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20443.1"
                     /translation="MTCTPMSARLSAALLWLIACPCLVAANTTSSGAAPAAAANEPAA
                     VLKHYINLRLAMADWPVYSKLITWPQEPASDCYWVTSHFKLDDPRPGSAHDEIIVPAT
                     YDRLGRYCSDFDLELFDESDRIEYHLVMSDGKWKIAAPLPDYPDIEIQTLLRRLANTA
                     QSPGETPERRRQLDEVIRQLRATLRAHPRVMRPR"
     gene            complement(1360..2208)
                     /locus_tag="VNZ06_06540"
     CDS             complement(1360..2208)
                     /locus_tag="VNZ06_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20444.1"
                     /translation="MLEPTAATALDEIPAETAETAESTESAVHWGAIIAGGVASAALT
                     LILLAFGAGVGFSAVSPWSAGSASTSFHLAAGLYFIVTAMLASSVGGYLAGRLRTRWT
                     GAQSREIFFRDTAHGFLAWGFATLLSAAVLSAASANLVGGVSATTLEAGGESAYVMDR
                     YVDELVRPQPGTGTVGAESMAERGIVRRIFAAGIHNKGEFAPADRLYLVQIVRDRQTL
                     GATQAQALVDAALADAQQTVIQMQKAAAQLALWLTASLFIGAFSASLAAIEGGALRDG
                     TWHYNV"
BASE COUNT          457 a          783 c          772 g          408 t
ORIGIN      
        1 cgcggtgaaa cgacgcctgg gcagaaagcg cagcaacgtg caacgcacat cggaaatgcg
       61 cgcccgcctg atgcaggccg ccatcgacac gctgtaccgc gccggctatt cggcgaccac
      121 cacgatcgag gtggcgcgcc gggcgcgcgt cagccgcggt gcgatgctgc atcattttcc
      181 gacccgcacc catctgctgc tggcgaccgc ccatcacatc gtggaaagtg atcgcttgtt
      241 tcgtcgcgag aaatttttcc ccatacggct cagcccaggc ctggagcgct tttacgccgc
      301 ctccgacgta agctgggagc tgcaccggcg cccgagcagc atagcgctgc tggagatcat
      361 gatggcgacg cgcagcgaca aggcgcttgc caaagcgttc gcgccatttc agaaaatgtg
      421 ggagcaagcc cggcacgctg cggccgcgag gatggcggcg gatctgggag tcgaggacct
      481 ggtcgcagtc acgaatctga ttcgtctgca tcaggcctgt atgcgcggcc tcgccatcga
      541 actgatgttc acgcatcacg cgcaggaagt ggaagccgca cgccagcttc aggtaggctt
      601 cgaccgcgca ttcgcgcaga atctggtcgc cgaagcgcgc aagcgtggga gccgcgcatg
      661 acctgcaccc ctatgagcgc tcgcctgagc gccgcattgc tgtggctgat agcgtgcccc
      721 tgcctggttg ccgccaatac aacctcatcc ggcgccgctc cggccgcggc cgccaatgaa
      781 cccgcggcgg tgctcaagca ctacatcaat ctgcggctgg cgatggcgga ctggccggtg
      841 tactcgaagc tcatcacctg gccgcaggag ccggcgtcgg actgctactg ggtgaccagt
      901 cacttcaagc tcgacgatcc gcgaccgggc tcggcgcacg atgagatcat cgtgccggcc
      961 acctatgatc gtctgggtcg ctactgttcg gacttcgatc tggagctgtt cgatgagtcg
     1021 gaccggatcg agtatcacct ggtcatgagt gatggcaagt ggaagatcgc ggcaccgctg
     1081 cccgactatc ccgatattga aatccagacg ttgctgcgaa gacttgccaa caccgcgcaa
     1141 tccccgggcg agactcccga acgccggcgt cagctcgatg aggtgatccg tcagctgcgg
     1201 gccacactgc gcgcgcatcc gcgcgtgatg cggcctcgct gaagcgcaac ctcacgcgcg
     1261 tcagcgcgat agcagcgcga tcagaatgat gatcgggatc ggaaccccca gcagataaag
     1321 caggatcgag cgtcccatgc agtgaatcct cctgccgtgt cagacgttgt agtgccaggt
     1381 accatcgcgc agcgcgccgc cttcgatggc ggcaaggctg gcggaaaatg cgccgatgaa
     1441 cagtgaggcg gtcagccaca gtgccagctg cgcggctgcc ttctgcatct ggatgacggt
     1501 ttgctgcgca tcggcgagcg cggcgtctac cagcgcctgc gcctgcgttg ccccgagggt
     1561 ctgccgatca cgcacgatct ggaccagata gaggcgatca gcgggcgcga actcaccctt
     1621 gttgtgaatt ccggcagcaa agatacgtct cacgatgccg cgctcggcca tgctttcggc
     1681 gccgacggtg ccggtgcccg gctgcgggcg taccagctca tcaacgtagc gatccatcac
     1741 gtatgcggat tcgccgccgg cctccagcgt ggtcgccgag acgcctccta ccaggttggc
     1801 ggatgcggcg ctgagcaccg cagcactcag cagcgtcgcg aatccccagg caagaaaccc
     1861 atgtgccgtg tcgcgaaaga aaatctcgcg cgactgggcg ccggtccatc gggtacgcag
     1921 tcgtcccgcc agatagccgc cgacgctcga ggccagcatc gccgtgacaa tgaagtaaag
     1981 gcccgctgcc agatggaagc tggtcgatgc gctacccgcg gaccagggtg aaacggctga
     2041 aaagccaact ccggcaccga aggcgagcag gatcagcgtg agtgccgcgg atgcgacacc
     2101 accggcgata atggcgcccc agtggaccgc cgattcagtg gactcggcag tctccgccgt
     2161 ctccgccggg atctcatcca gggccgttgc agccgtcggc tctaacatgc gcgctactcc
     2221 cgcgggtgcg tatgggctag cgttataggc gcggctcctg aagctcaata gcggcacagc
     2281 agagaagtga tagtcagccc ttacctacgc ctgcgaggct tggctgtggc accatcggga
     2341 catgacgatg atccgttgtt cggaatgcgg ccgcatgatc agcgatcagg ctgcggcctg
     2401 cgtgggttgc ggcgcaccgg
//
LOCUS       DATMRM010000222         8969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1700140, whole genome shotgun sequence.
ACCESSION   DATMRM010000222 DATMRM010000000
VERSION     DATMRM010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8969
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700140"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VNZ06_06545"
     CDS             complement(<1..518)
                     /locus_tag="VNZ06_06545"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HWW20445.1"
                     /translation="MSSHVKDLLTLEGEAFVRAAYLTLLGREPDPGGLGNYVRELNAG
                     VRKLAILSKLRNSDEGRRHRATLRGYRGAQVRQWLHARAEGSGRVSPSLTRTTSDDGV
                     WLDVLGILDGVDKASLRGEIHCSWDYLRHYAHALRIYRDEAINVIEIGVAAGASLKMW
                     LRYFSRATVVGV"
     gene            complement(683..2479)
                     /gene="aceK"
                     /locus_tag="VNZ06_06550"
     CDS             complement(683..2479)
                     /gene="aceK"
                     /locus_tag="VNZ06_06550"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HWW20446.1"
                     /translation="MRFYHPDAAAAEGALSSTGAARPRGQVANAFESALYAMRCAQMV
                     VEAFLTYNANFREITRRAQGNFATREWARSHHDAVERIELYEQSIQSIVAQLELALGR
                     NVREPALWSDVKRHFSNLISGLPDGEFCKTYFSSVTRRLFGTVGVTPDIEFVATDLDP
                     LSNARPASVVHRYPIEGELTHTVRDMLASRALGAPWVDLDASAWLVASAGERLLWQRN
                     ERRDIEALEIIDAVFYQSTRAYIVGRIVGRGFAAPLAIALRSTEAGIVVDTTMLVEDE
                     LSILFGFARSYFQVDLESVADTVAFLKELMPRKQAGELFTVLGRAKQGKTERYRSLFR
                     HLSSSSDQFNHAPGQKGMVMICFTLPSFEVVFKVIRDHIPEVKSVSKKDVMAKYSFVF
                     LHDRAGRLIDAQEFRRLRFPLKRFSAELLQELREQAANTVHIEGDDLIIDHCYVERRV
                     TPLDVYLLNASEPAARRAAVDYGRAIRDLAYTDVFPGDLLLKNFGVTRHGRVVFYDYD
                     ELCRVTDCQFRDLPTASNDEDEMRAEPWFFVGDKDVFPESFVNFLGMSGELRQEFLQV
                     HGDLFEASFWRRTQQRIAAGEVLEVLPYVPME"
     gene            complement(2481..3776)
                     /gene="aceA"
                     /locus_tag="VNZ06_06555"
     CDS             complement(2481..3776)
                     /gene="aceA"
                     /locus_tag="VNZ06_06555"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002554010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HWW20447.1"
                     /translation="MQSRSLPTADEIRRNWQESPRWRGITRAYKAEDVVRLRGTIPVE
                     HSVARITAEKLWRYVNEKPFVNALGALTGNQAMQQVKAGLDAIYLSGWQVAGDANLAG
                     EMYPDQSLYPADSVPAVVRKINNTLRRADELYHAEGNDSIDWLKPIVADAEAGFGGVL
                     NAFELMKGMIDAGAAGVHFEDQLSSAKKCGHMGGKVLVPTQEGINKLVAARLAADVCN
                     VPTVLIARTDAESANLLTADVDERDRAFIDQTKARTAEGFFHVKCGMDQAISRALAYA
                     PYVDMIWCETGHPDLEEAKQFAEGVHQQFPGKLLAYNCSPSFNWKRKLDDATIAKFQR
                     ELGAMGYKFQFITLAGFHALNYSMFELAQGYKSSQMTAYVALQQAEFAAEPRGYSATK
                     HQREVGAGYFDDVTQTVTAGTSSLSALSGSTEEQQFRSA"
     gene            complement(3806..5443)
                     /gene="aceB"
                     /locus_tag="VNZ06_06560"
     CDS             complement(3806..5443)
                     /gene="aceB"
                     /locus_tag="VNZ06_06560"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HWW20448.1"
                     /translation="MNTQLQSDPGLYTQRLRGLVPGVDMTRQRVERMDDALSERAVKL
                     LALLHRRFNSRRLELLAARDRRQGELDQGTLPGFLPETAAIRAGDWRIAPVPHDLIDR
                     RVEITGPAERRTIINALNAPVRAFMADFEDALTPSWQNVIDGQVALNDAIRRRIVYSD
                     STSGKLYRINDKVATLIVRPRGWHLPEKHVRIDGEPVSGALFDFALYLANNHEELARN
                     GSGPYFYLPKLENHLEARLWNDVFVAAQDQLGMAHGTIKATVLIETILAAFEMDEILY
                     ELREHVTGLNCGRWDYIFSYIKKFRNRPHALLPDRAAISMDKAFLKSYVDLLIRTCHR
                     RGAHAMGGMAAQIPSRGDPQASAAAMANVRADKLREARAGHDGTWIAHPGLASVANEV
                     FNALMPGPNQLNVLRPDVKVSAADLLQTPSGAITEAGMRHNIRVGVRYIESWLGGTGC
                     VPLYHLMEDAATAEICRAQLWQWLRHGAHTDDGRPITVERFDRALSDEIERIHREVGG
                     ARMLAGVFPSAARLFAGMVTSESFDEFLTLPAYELLG"
     gene            5513..7072
                     /locus_tag="VNZ06_06565"
     CDS             5513..7072
                     /locus_tag="VNZ06_06565"
                     /inference="COORDINATES: protein motif:HMM:NF023689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3612 domain-containing protein"
                     /protein_id="HWW20449.1"
                     /translation="MTNLLRKGHFLGAKLRSIRKRNGLTLEELSSRCIQMDVGSAPSV
                     SYLSMIESGKRMPSEELLQLLAGVFQRDVHWFLDEQEDVGAAIETPRAGMTRVPLQPA
                     FLFSKELLQTAIPELLTQTGTTGRQFAHLLIRSHQEMSRNDFPDLERTAESIGERRFP
                     LNVEDLVRLAKHHGLELRWFERRPLLVRDNDQEVRSTVRSFFEPPGTIYLNQALRSDP
                     ARLKFDLATHIGHKVLHGGDGMKSPHATGGEMGGSPESGSEAGLRAQDVLHAWRDFEC
                     SFFAGALLCPKIAFRRFLARNQYRLEPATQLELTPAVVMRRMTKVSPYPYWHFFDAYP
                     PGYLRAVYRGNGIPLPWGNMAQVSDPCPQWAVFRMIDGHQKESGSQISVLRDHDRSLL
                     YCCHSLRTRDMAGNYHVLSVGIDLAPALDANGTESSQVIQDITRECLHRRGEGAMPRD
                     AAIAVRAAARVLNIAWIEEALTHPPRIICPRSSRCPRPERCESALPSRAREVTDVRAE
                     ILGRLRAGSTE"
     gene            complement(7078..8028)
                     /locus_tag="VNZ06_06570"
     CDS             complement(7078..8028)
                     /locus_tag="VNZ06_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HWW20450.1"
                     /translation="MSRILIVGEHAQGKLNVSTAKCVNCAKAIPDAQIDVVLLGSELS
                     SVAAQAAALDGVQKVLSVERSENAEPLAAVFAPQIAALAKGYGHVFGPSTTYGKDLMP
                     RVAALLGVSQISDIMAVESATRFRRPVYASNVILTVEAPAGKTVVATVRTASFQPTGS
                     AGKAAIEATKVEAALPTYTRFVSRSQASGDRPDLQTAARVVSGGRALASAENFKLLYR
                     LADALGAAVGASRAAVDAGYAPNELQVGQTGKIIAPELYIALGISGAIQHLTGIKDAR
                     TIVAINKDSEAPIFEVADVGLVGDLFALLPELEQAILAAK"
     gene            complement(8029..8784)
                     /locus_tag="VNZ06_06575"
     CDS             complement(8029..8784)
                     /locus_tag="VNZ06_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HWW20451.1"
                     /translation="MSSPIIVGIKRVIDYSVRVRVKPDGTGVVTDGVKMSINPFDEIA
                     LEEALRIKERNLAGEIIAVSIGPSDAQQQLRTALAMGADRAILVQVDQPAEPLTAARV
                     FLKLAQKHSPQLVILGKQAIDDDNGQTAQMLAALWDRPQATFASKLELDGGKARVTRE
                     VDVGLETIEVDLPAVVSTDLRLNEPRYVKLPDIMKAKKKPLDVTSLSDLGVEVSATGI
                     RTLKFEAPAARSRGVIVKSVPELIAALKQRGVL"
     gene            complement(8787..>8969)
                     /locus_tag="VNZ06_06580"
     CDS             complement(8787..>8969)
                     /locus_tag="VNZ06_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20452.1"
                     /translation="HRLRRAEGLPLLQQKFEAGVRGHFGTQQAEQIVKMFADRAAFEG
                     MAVNDMMTALVHTGVN"
BASE COUNT         1667 a         2916 c         2697 g         1689 t
ORIGIN      
        1 tctactccta ccacggtggc cctgctgaaa tagcgcagcc acattttcag tgatgcaccc
       61 gcggcgacgc cgatctcgat cacgttgatt gcctcatccc gatagatgcg caaggcatgg
      121 gcgtaatgtc gtagatagtc ccaggagcaa tgaatctcgc ccctgagaga tgccttatcc
      181 acgccgtcga gaatgcccag cacgtccagc cacacgccgt catcagatgt cgtgcgagtc
      241 aggcttgggg acacgcgtcc gcttccctct gctcgcgcgt gcagccattg gcgaacctgc
      301 gcgccacgat acccgcgcag ggtcgcgcgg tgccgtcgtc cctcatcgga attccgcaac
      361 tttgagagga tggcgagctt gcgtacgccc gcgttcaact ctcgaacata attacctaat
      421 ccacccgggt ccggctccct gcccagcaga gtcaagtagg cagcacggac gaatgcttcg
      481 ccttcgagcg tcagcagatc tttgacatgg ctcgacacaa aatcccctgt ctcagtatgt
      541 cgccgcctgc accttgcggt ggcctgaaca ggcttcgacc gaagacgact tggacatttg
      601 tgacagcgct gattatagcg acagccgacg ccggcgccgt ggtttggctt tcaacgactc
      661 aagtgtattt gacctagtgg gactattcca tcggcacgta cggcagtact tcgagcactt
      721 cgccggccgc gatacgctgc tgagtgcgcc gccagaatga ggcctcgaac agatcgccgt
      781 gcacctgcag aaactcctga cgcagctcac ccgacatacc gaggaagttc acgaagctct
      841 ccggaaacac gtccttgtcg ccgacgaaaa accacggctc ggcgcgcatc tcatcctcat
      901 cattgctcgc ggtcggcaga tcgcggaact gacagtcagt cacgcggcac agttcatcgt
      961 agtcatagaa gacgacgcgg ccatggcgcg tgacgccaaa gttcttcagc aacagatcgc
     1021 ccggaaacac atcggtatag gccagatcgc ggattgcacg gccatagtcc accgcggcgc
     1081 gccgcgcagc gggctcggaa gcgttgagca gatagacatc cagcggcgtc acacgccgct
     1141 cgacgtagca atgatcgatg atcagatcgt cgccttcgat gtgcaccgtg ttggccgcct
     1201 gttcgcgcaa ctcctgcagc aattcggccg aaaaccgctt caacggaaag cgcagacgac
     1261 gaaattcctg cgcatcgatc agccgaccgg cccgatcatg caggaacacg aagctgtatt
     1321 tggccatcac atcctttttc gagacactct tcacctcggg tatgtgatcg cggatgacct
     1381 tgaataccac ctcaaatgac ggcagcgtga agcagatcat caccatgccc ttctgaccgg
     1441 gggcgtgatt gaattgatcg ctgctgctgc tgagatgccg gaacagactg cggtagcgtt
     1501 cggtcttgcc ctgtttggcg cgcccgagca cggtgaagag ctcacccgcc tgtttgcgcg
     1561 gcatcagctc cttcaggaag gcaacagtgt cggcgaccga ctccagatcg acctggaagt
     1621 agctgcgcgc gaagccgaac agaatgctga gctcatcttc gaccagcatc gtggtatcca
     1681 ccacgatccc ggcctcggtg ctgcgcagcg cgatggccag tggagcggcg aacccgcggc
     1741 ccacgatgcg tcccacgatg taggcgcgcg ttgactgata gaacaccgca tcgatgattt
     1801 ccagcgcctc gatgtcgcgg cgctcgttgc gctgccacag gagccgctcg ccggccgaag
     1861 cgaccagcca ggcgctcgca tccagatcca cccacggtgc gcccaaggcg cgcgaggcca
     1921 gcatgtcccg gacggtatgc gtgagctcgc cttcgatcgg atagcgatgt acgacgctcg
     1981 ccggcctggc attcgataac ggatccaggt cggtcgcgac gaattcaata tcgggcgtga
     2041 cgccgacggt gccgaacaac cgccgcgtca cggagctaaa gtacgttttg cagaactcgc
     2101 cgtccggcaa gcccgagatg agattgctga aatggcgttt gacgtcgctc cacaacgccg
     2161 gctcgcgtac gttacgtccc aacgccagct cgagctgggc cacgatcgac tggatgctct
     2221 gttcgtatag ttcgatgcgc tcgacggcat cgtggtgact gcgggcccat tcacgcgtcg
     2281 caaaattccc ctgggcgcgg cgtgtgattt cgcggaagtt ggcgttgtag gtcaggaagg
     2341 cctcgacgac catctgtgcg caacgcatcg cgtacaacgc cgactcgaag gcgttggcga
     2401 cctgaccccg cgggcgagcg gctccagtag agctcagcgc tccctctgca gccgccgcat
     2461 cggggtgata gaaccgcatc ctaggccgag cggaactgct gctcctcggt gctgccgctc
     2521 agtgcgctga gcgacgaggt gccggcggtc acggtctgcg tcacatcgtc gaagtacccc
     2581 gcgccgactt cacgctgatg cttggtggcg ctatagccac gtggctcggc ggcaaactcc
     2641 gcctgctgca gggcgacgta ggcggtcatc tggctgctct tgtagccctg cgccagctcg
     2701 aacatcgagt agttgagcgc gtggaagccg gccaacgtga tgaactggaa cttgtagccc
     2761 atcgcaccca gctcgcgctg gaacttggcg atcgtcgcgt catcgagctt gcgcttccag
     2821 ttgaatgacg gcgagcagtt gtacgccagc agcttgcccg ggaactgctg atgaacccct
     2881 tcggcgaact gcttggcctc ttcgaggtcc ggatgcccgg tctcacacca gatcatgtcc
     2941 acgtacggcg catacgccag agcgcgcgaa atcgcctggt ccataccgca cttcacgtgg
     3001 aaaaaccctt ccgcggtgcg agccttggtt tggtcgatga aggcgcgatc gcgctcatcc
     3061 acgtccgcgg tcagcaggtt ggccgattcc gcatcggtac gggcgatcag cacggtcggc
     3121 acgttgcaca cgtcagccgc caggcgcgct gccacgagtt tgttgatgcc ctcctgcgtc
     3181 ggcaccagca ccttgccacc catgtggccg cacttcttgg ccgaagacag ctggtcctcg
     3241 aagtgcacgc cagccgcgcc ggcatcgatc atgcctttca tcagctcgaa cgcattgaga
     3301 acgccgccga aaccggcctc ggcatccgca acgatcggct tcagccagtc gatgctgtca
     3361 ttgccttcag cgtggtagag ctcatcagcg cggcgcaggg tgttgttgat cttgcgcacc
     3421 acggccggta ctgagtcggc cggatacagc gactggtccg gatacatttc accggcaaga
     3481 ttcgcatcgc cggcgacctg ccaccccgac agatagatcg catcaaggcc agccttcact
     3541 tgctgcatcg cctgattgcc ggtcagtgcg ccgagcgcgt tgacgaacgg cttttcgttc
     3601 acatagcgcc acagcttctc ggcagtaata cgggcgaccg aatgttccac cggaatcgtt
     3661 ccacgcaacc gcacgacatc ctcggctttg taggcgcgcg tgataccgcg ccagcgggga
     3721 ctctcctgcc agttacggcg aatctcatcg gcggtgggaa ggcttcgtga ttgcatcgtc
     3781 tttgactcct ggatatccaa acctgtcagc ccagcaactc gtacgcgggc agtgttagaa
     3841 actcgtcgaa gctctcactg gtgaccatgc cggcgaacag ccgcgcggcg ctcggaaaga
     3901 cgcccgccag catgcgcgct ccgccgactt cccggtgaat gcgctcgatc tcatcgctta
     3961 gggcacgatc gaagcgctca acggtaatcg gtctgccgtc gtcggtgtgc gcgccatgac
     4021 gtaaccactg ccataactgc gcgcggcaga tctcagcggt cgcggcatct tccataagat
     4081 ggtagagcgg cacacagccg gttccgccga gccacgattc aatgtagcgc acgccgacgc
     4141 gaatgttgtg gcgcatcccg gcctcggtga tcgcgccgga cggggtctgc agtagatccg
     4201 cggcactgac cttcacatcc ggccgcagca cgttcagctg gttgggcccc ggcatgaggg
     4261 cgttgaacac ttcattcgcc accgaggcaa gtccgggatg cgcgatccag gtgccatcat
     4321 gaccggcgcg cgcctcgcgc agcttgtcag cgcgcacgtt ggccatcgcc gcggcgctgg
     4381 cctgcggatc gccacgcgat ggaatctggg ccgccatgcc acccatggca tgcgcaccac
     4441 ggcggtgaca ggtccggatc agcagatcga cataggattt caggaacgcc ttgtccatcg
     4501 agatcgccgc tcgatccggc agcagcgcgt gcggccggtt cctgaatttc ttgatgtagc
     4561 tgaaaatgta gtcccagcgg ccgcaattca ggccggtgac gtgctcgcgc agctcgtaga
     4621 ggatttcgtc catctcgaac gcggccagaa tcgtctcgat caataccgtg gccttgatcg
     4681 tgccatgggc catgccaagc tgatcctgcg cggccacgaa cacgtcgttc cagaggcgcg
     4741 cctcgagatg attttcgagc ttcggcaagt agaagtacgg gccggatccg ttgcgagcca
     4801 gctcctcgtg gttgttggcc aggtacagcg caaagtcaaa cagcgctccg gacaccggct
     4861 cgccgtcgat gcgcacatgt ttttccggca gatgccagcc gcgcggccgc acgatcagcg
     4921 tcgcgacctt gtcgttgatc cgatagagct tgccggaggt cgaatcgcta tagacgatcc
     4981 gccggcggat cgcatcgttc agcgcaacct gcccgtcaat gacgttctgc cagctgggcg
     5041 tcagcgcgtc ttcgaaatcg gccatgaagg cgcgcaccgg cgcgttcagg gcgttgatga
     5101 tcgtcctgcg ctcggccggc ccggtgattt ccacgcgccg gtcgatcaaa tcgtggggca
     5161 ccggcgcgat gcgccagtcc ccggcacgga tcgcagcggt ctcaggcaaa aagcccggaa
     5221 gcgtgccctg gtcgagctcg ccctggcgcc ggtcgcgtgc cgcgagcagc tccagacgcc
     5281 ttgaattgaa gcgtcggtgc agcagcgcca gcagcttcac ggcacgctcg gagagtgcat
     5341 cgtccatgcg ctcaacgcgt tgccgtgtca tatccacgcc gggcaccagt ccccgcaaac
     5401 gctgggtgta gaggcctgga tcggactgca gctgggtgtt cacggtaaca gcatacgggt
     5461 caaattgagc aatatttgac tcacaaaact tcacagtgtt aatttcacac ccatgaccaa
     5521 tctgttacgc aagggtcatt tcctcggggc gaagttgcgc agtatccgta agcgcaacgg
     5581 cctgacgctg gaggaactgt ccagccgctg cattcagatg gatgtcggca gcgccccctc
     5641 cgtgtcctac ctgagcatga tcgagagcgg taagaggatg ccgtcagagg agctgctgca
     5701 gttgctggca ggggttttcc agcgcgacgt gcactggttt ctcgacgagc aggaggatgt
     5761 cggcgcggcg atcgaaacgc cgcgggcggg catgacgcgc gtgccgttgc agccggcctt
     5821 cctgttctcc aaagagctgc tgcagaccgc gattccggaa ctgctcaccc agaccggcac
     5881 cacggggcgg cagttcgcac acctgttgat ccgctcgcac caggaaatgt cgcgcaacga
     5941 ttttccggat ctggaacgca ctgccgagag catcggtgaa cggcgcttcc cactgaatgt
     6001 ggaggatctg gtgcggctcg ccaaacatca tggtctggag ctgcgctggt tcgagcggcg
     6061 accgctgctg gtacgtgaca acgatcagga agtgcgcagc accgtacgct cgttcttcga
     6121 gcctcccggc accatctatc tcaatcaggc gctgcgatcc gatcccgcgc gactgaaatt
     6181 cgatctcgcc acgcacatcg gtcacaaggt gctgcacggt ggtgacggca tgaaatcgcc
     6241 gcacgcgacc ggtggcgaaa tgggtggcag cccggagagc ggttcggaag ccggcctgcg
     6301 ggcgcaggac gtgctgcacg cgtggcgcga cttcgaatgc agcttttttg ccggtgcgct
     6361 gctgtgcccg aagattgcgt tccgtcgttt cctcgcgcgc aatcaatatc ggctggaacc
     6421 cgcgacgcag ctggaactca cccccgcggt cgtgatgcgc cggatgacga aagtgtcacc
     6481 ctacccgtac tggcatttct tcgatgccta cccgccgggc tacctgcgcg cggtctatcg
     6541 cggcaacggc atcccgctgc cctggggcaa catggcgcag gtcagcgatc cgtgcccgca
     6601 atgggcggta tttcgcatga tcgacggaca tcagaaagaa tccggctcgc agatctcggt
     6661 gctgcgtgat cacgatcgct cgctgctgta ttgctgccat tcgctgcgca cgcgcgacat
     6721 ggctggcaac tatcacgtac tgtcggttgg gatcgatctg gcgccagcgc tggatgccaa
     6781 tggcaccgaa tcatctcagg tcattcagga catcacgcgc gagtgcctgc accgacgtgg
     6841 cgagggcgcc atgccgcgcg atgcggccat tgcggtgcgc gcggctgcac gcgtgctcaa
     6901 catcgcctgg atcgaggaag cgctcacgca cccgccgcgc atcatctgtc cgcgcagcag
     6961 ccgctgtccg cggcccgagc gctgcgagtc cgcgttaccg tcgcgcgcac gggaggtcac
     7021 cgacgtgcgg gcagagattc tgggcaggct gcgcgccggc tcgaccgaat agcgcggcta
     7081 tttcgccgcg agtatcgcct gttccagctc cggcagcagc gcgaacagat cgccgaccag
     7141 gccgacgtcc gcgacctcga aaatcggcgc ttccgaatcc ttgttgatcg cgacgatggt
     7201 acgcgcatcc ttgatgccgg tcagatgctg gatcgcgccc gagatgccca gcgcaatgta
     7261 cagctccggt gcaatgatct tgccggtctg tcccacctgc aattcgttgg gcgcataacc
     7321 ggcgtccacg gcggcgcgtg aggcaccgac ggcggcaccg agcgcatcgg cgagtcgata
     7381 gagcagtttg aaattttcag cgctcgccag cgcccgcccc ccggagacca cgcgggccgc
     7441 ggtctgaaga tcgggccggt cgccactggc ctgcgagcgc gagacgaagc gagtgtaggt
     7501 cggaagggcg gcctccactt tcgtcgcttc aatcgccgcc ttgccagcgc tgccggtggg
     7561 ctgaaacgat gcggtgcgaa cggtcgcgac cactgttttc cccgccggag cttcgaccgt
     7621 cagaatcacg ttgctggcgt acaccggacg acgaaaccgg gtcgccgact ccactgccat
     7681 gatgtcgctg atctgactga cgcccagcag ggctgccaca cgtggcatca ggtccttacc
     7741 atacgtggtc gacggtccga acacgtggcc ataacccttg gccaatgccg cgatctgcgg
     7801 tgcgaacacg gcagccagcg gttcagcatt ctccgagcgc tcgacgctca gtaccttctg
     7861 caccccgtcc agggcagcgg cctgtgctgc gaccgaactg agctccgagc cgagcagcac
     7921 cacatcgatc tgcgcatcgg gaatcgcctt cgcgcagttc acgcacttgg cggtgctgac
     7981 gttcagcttg ccctgggcgt gctcgccaac gatcagaatc ctgctcattt aaagtactcc
     8041 gcgctgcttc agcgccgcga tcagttccgg cacgctcttc acgatcacgc cgcgcgagcg
     8101 cgcagccggc gcctcgaact tgagcgtacg gatgccagtc gccgaaacct cgacacccag
     8161 atccgacagg ctcgtgacat cgagcggctt cttcttggcc ttcatgatgt ccggcagctt
     8221 gacgtagcgc ggctcattca accgcaggtc ggtactgacc acggccggca gatcaacttc
     8281 gatggtttcc agtcccacgt ccacttcgcg cgtcacccgc gccttgccac cgtcgagttc
     8341 gagtttggag gcaaaggtgg cctgcgggcg atcccacagc gcagcgagca tctgcgccgt
     8401 ctggccattg tcatcgtcga tcgcctgttt gcccaggatc accagctgcg gcgagtgctt
     8461 ttgtgccagc ttcaggaaca cgcgcgccgc ggtcagtggc tccgccggct gatcgacctg
     8521 aaccaggatc gcgcgatccg cacccatcgc cagcgcggtg cgcagctgct gctgcgcgtc
     8581 ggacggcccg atgctcacag cgatgatttc gccggccagg ttgcgctcct tgatgcgtag
     8641 cgcctcttcc agagcgattt catcaaacgg gttgatgctc atcttgacgc cgtcggtgac
     8701 gacgccggtg ccatccggtt tgacgcgcac ccgcacgctg tagtcgataa cccgcttgat
     8761 cccaacaatg ataggactgc tcatgctcaa ttgactccgg tgtgaaccag cgccgtcatc
     8821 atgtcgttga cggccatgcc ttcgaatgcc gcgcgatcgg caaacatctt gacgatttgc
     8881 tcggcctgct gcgtcccgaa atggccacgc acgcccgctt cgaatttctg ttgcagcagc
     8941 ggcaagcctt ccgcgcgccg caaccggtg
//
LOCUS       DATMRM010000223         5590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2914417, whole genome shotgun sequence.
ACCESSION   DATMRM010000223 DATMRM010000000
VERSION     DATMRM010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2914417"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VNZ06_06585"
     CDS             <1..274
                     /locus_tag="VNZ06_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20453.1"
                     /translation="TQLQAIIFSGPFKRTHTSVTMHQFRQITPDVILVEALREVTGFK
                     SLPAGISPTEPGVLKTRMKYVLVKRDELWQIVAAQDTAILPPPPAR"
     gene            complement(424..2373)
                     /locus_tag="VNZ06_06590"
     CDS             complement(424..2373)
                     /locus_tag="VNZ06_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWW20454.1"
                     /translation="MLAPLAFCPSPARADGNDATIGELKTLSVEELMDIPVTSVARHP
                     EKLLEAASAVQVITQEDIRRSGATSIAEALRLADNLQVAQKNSHDWAISARGFNTALA
                     NKLLVMIDGRTVYTPLFSGVFWDIQDYLLEDIDRIEVISGPGGTLWGANAVNGVINII
                     TKSAKDTQGSFAEGGGGSEPKGIAGARYGGSLSSNTSFRVYGKYIDRGDEALADGEAV
                     SDSWHQERGGFRLDADPSVRDALTLQGDFYDGREDEQTGGTATTSGGNLLGRWARKFS
                     DDSDISLQSYLDQTHLLDPEPALILNHRPLAPAGTLQDDLTTYDIDFQHRFALGSDHR
                     FVWGLGYRYTHDVVGNAPAVGFLPPRLDHSLYSGFAQDEIALQPDLSFVLGTKLEHND
                     YTGWELQPNARLRWTFMEGQAVWAAVSRAVRTPSRVDRDLYEAPPPRFLLIAGSPRFD
                     SEKLLAYELGYRAQFTSRFSSAVSTFYNVYSDVRSTSITPRTVLPFYFANNLEGHTYG
                     LEFSGDYQLLDNWSLHGGYTLLREHLRVKPGQVDINDALNETADPEHQFSLRSSLQLS
                     RQVQFDLSLRWVDSLTLNTGPTRGVVPSYAELDTRVAWQATRRFELSLAGQNLLHNRH
                     PEYGFPDPNRVEISRSVYAKLTWRQ"
     gene            complement(2613..3512)
                     /locus_tag="VNZ06_06595"
     CDS             complement(2613..3512)
                     /locus_tag="VNZ06_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HWW20455.1"
                     /translation="MNAVTSFLVFVFLAAAMGAGYFVSPYLGAVLFAAAVLIGMSLKM
                     ANVWQKFVILRMGKLHSVKGAGLFTIIPVLDAVVAVIDQRIQTTAFNAEQALTRDTVP
                     VNVDAIIFWHVHDAEKAALAITNYREAIDRVAQTSLREMIGSSMLAALLSDRKDADEQ
                     LKLEIGLKTAPWGISVSSVEIRDVAIPVALQDAMSRQAQAEREKQARVILGSAEAAIA
                     GKFVEAANVYAGHPEALQLRAMNIIYETTKERGATILMPSSMVDSMNPYGSTLALTLA
                     GNEVFKPNSMLKVADPARPAVAV"
     gene            complement(3636..4046)
                     /locus_tag="VNZ06_06600"
     CDS             complement(3636..4046)
                     /locus_tag="VNZ06_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20456.1"
                     /translation="MLSVLSALARLDVDPWEEASRLAGLPRPAAIQFLMALIVALPDG
                     SSARSNPEMHAQRLTALLPARGVTNPPASKAEIATGISTYHQGFVRYVLFYLVLMAFF
                     FGGQWLIEHSPRPGHSGTVTSPESGAALPHTPAN"
     gene            4337..>5590
                     /locus_tag="VNZ06_06605"
     CDS             4337..>5590
                     /locus_tag="VNZ06_06605"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWW20457.1"
                     /translation="MMQRTESDGALFEENERAEATLNSIADAVISTDVRGYVTYLNVV
                     AERLTGWSRQEAAGRPLEEVLRIVDDSTRNPAPNPVALAIRENLSVALAANCVLVRRD
                     GIEAAIEDSTAPIHDRDGQVTGAVMVFHDVSHARSMSLKMSHLAQHDSLTDLPNRVLL
                     NDRLKCAMANARRHQQRLAVLFLDLDRFKQINDSLGHGVGDRLLKSVAQRLSGCIRRA
                     DTISRQGGDEFIFLLSELAHAHDAAVCAEKILAALSTPHAVDQHALYVTASIGIATFP
                     DDGTDADTLLRHADIAMHSAKVDGRNSYQFFRPEMNTRVIDRQLLERSLREAIERDEF
                     VLHYQPIVNLQTRAIVAVEALARWRHPRRGLLSPAEFMPLAEECGAIVSIGAWALREA
                     CRQAVVWQRAKLQPIRVAVNVSAAEL"
BASE COUNT         1136 a         1682 c         1720 g         1052 t
ORIGIN      
        1 cacgcaattg caggcgatca ttttcagcgg tccgttcaag cggacgcata cctcggtcac
       61 gatgcaccag ttcaggcaga tcacacccga cgtgatattg gtggaagcac tgcgtgaagt
      121 aaccggattc aagtcactgc ccgctggtat ctcgccgacg gaacccggcg ttctgaagac
      181 ccgcatgaag tacgtgctcg tgaaacgcga cgagctctgg cagatcgttg ccgcccaaga
      241 taccgccata ctgccgccgc caccggcgcg ctaggatcgc acgctcatcc tgagcacgcg
      301 caatcgacga cgtggcgcgg gatgggacgg ccagcgacgc aaccgatagg cgttcagaaa
      361 aatgtcgcct gcggcgagcc tcaaacgtcc ttggagtgag cgcgctgact cgggtcggat
      421 cgactactgg cgccacgtca gcttggcgta gacactgcgc gagatctcga ctcggttcgg
      481 atcggggaag ccatactcgg gatgacgatt gtgcagcagg ttctgcccgg cgagtgacag
      541 ttcaaaccgg cgagtcgcct gccaggccac gcgtgtatcg agctcagcat agctgggcac
      601 cacacctcgc gtcggtccgg tattgagggt caatgaatcc acccagcgca ggctcaggtc
      661 gaactgcacc tgtcgcgata gttgcagcga cgagcgcaac gagaactggt gctccgggtc
      721 ggcggtttcg ttgagcgcat cattgatgtc cacctggccc ggtttgacgc gcaagtgttc
      781 tctgagcaac gtgtagccgc cgtgcaggga ccagttgtcc agtagctggt agtcgccgct
      841 gaattccagc ccataggtgt gcccttccag attgttggcg aagtagaacg gcagcacggt
      901 cctcggggta atgctggtgc tgcgcacgtc gctgtatacg ttgtagaagg tcgagaccgc
      961 actgctgaag cgcgaggtga actgcgcgcg gtagcccagc tcgtaggcga ggagcttctc
     1021 ggaatcgaaa cgcggactcc ccgcgatcag tagaaaccgc ggcggaggcg cctcgtagag
     1081 atcccggtcc acgcgcgaag gcgtgcgcac ggcccgcgac acggcagccc agacggcctg
     1141 tccttccatg aaggtccagc gtaagcgagc attgggctgc agttcccagc cggtgtagtc
     1201 gttgtgctcg agcttggtgc caagaacgaa ggataagtct ggctggagcg cgatctcatc
     1261 ctgcgcgaac ccactgtaca gactgtggtc caagcgcggc ggcagaaagc caactgccgg
     1321 agcgttgcca acgacatcgt gcgtgtagcg gtagcccaaa ccccacacga atcgatggtc
     1381 gctccccagt gcgaaacgat gctggaagtc aatgtcgtaa gtcgtgaggt cgtcttgcag
     1441 cgttccggcc ggcgccagtg gccggtgatt gaggatcaat gccggctccg gatccagcag
     1501 atgcgtctgg tcgaggtagg actgcagact gatgtccgaa tcatcggaga acttgcgagc
     1561 ccagcggcca agcaaattgc cgccactggt cgtagcggta cccccggtct gctcatcctc
     1621 gcgaccgtca tagaagtcac cctgcagggt cagggcatcc cgaacggaag ggtccgcatc
     1681 cagacggaaa ccgccgcgct cctgatgcca ggaatccgaa accgcctcgc catcggccag
     1741 tgcctcatcg ccgcgatcaa tatatttgcc gtacacgcgg aacgaggtgt tggatgagag
     1801 tgagccgccg tagcgggcgc ccgcgatgcc cttcggttca gagccaccgc cgccttcggc
     1861 gaagctgccc tgcgtatctt tggcactctt ggtaatgatg ttgatcacac cattgactgc
     1921 gtttgctccc cacagcgtgc caccggggcc gctgatgact tcgatgcggt cgatgtcttc
     1981 cagcaggtag tcctggatat cccagaacac tcctgagaac agcggtgtgt agacggttcg
     2041 tccgtcgatc atgacgagca gcttattggc caaagcagta ttgaagccgc gcgcgctgat
     2101 ggcccaatcg tgagaattct tttgcgccac ctgcagattg tcggccagcc gcagtgcctc
     2161 agcgatgctg gtggctccgg agcgccggat gtcctcctgc gtgatgacct gcaccgcgga
     2221 agccgcctcg agcaactttt ccggatgccg tgcgaccgaa gtcaccggaa tatccatcag
     2281 ctcctcgacg ctgagcgtct tgagttcacc gatcgtagcg tcgttaccgt cggcgcgcgc
     2341 cggtgagggg cagaaggcca gcggagcaag cattgagagc acacacgcac atcgggacag
     2401 gatgggtgtg cgtcggtgta cacacaaatt gctcggagcg gaatcgagat tcgctgccac
     2461 agtcgctgct tccttgccat ctcggcctct caagcgccgc agcgtgcgcg tgaggaatcg
     2521 ggctgcatag cccgtgatcc ggattatgtg agtggcgtct caggaagacc cccgcgatcg
     2581 gtaagcaatc cgatacccgc gccagggccg cgtcacacgg cgaccgcggg tcgcgctggg
     2641 tccgccacct tgagcatgga atttggcttg aatacttcat ttcccgcaag ggtgagggcc
     2701 agcgtgctgc catagggatt catgctgtcg accatcgagc tgggcatcag aatcgtggcg
     2761 cctcgctcct tggtggtctc atagatgatg ttcatggctc gcagctgcag cgcttctgga
     2821 tgcccggcgt agacattggc agcttcgaca aacttgcccg cgatcgccgc ttcggcggac
     2881 ccgagaatca cccgcgcctg tttctcgcgt tcagcctgag cctgacgcga catcgcatct
     2941 tgcagtgcca ccgggatcgc gacgtcgcga atctcgaccg agctcaccga gatgccccag
     3001 ggtgcagtct tgaggccaat ttcgagcttg agctgctcgt ctgcgtcctt gcgatcggat
     3061 agcaatgccg ccagcatgga agagccgatc atctcgcgca aggaggtctg tgcaaccctg
     3121 tcaatcgctt cgcggtagtt ggtgatggcc aatgcggcct tctctgcgtc gtgcacatgc
     3181 cagaagatga tggcatcgac attgaccggg accgtgtccc tggtcagcgc ctgctcggcg
     3241 ttgaacgcgg tggtctgtat ccgctggtcg atgaccgcca ccaccgcgtc gagcaccggg
     3301 atgatcgtga acagccccgc tcctttgacg ctatgcagct tccccatcct gaggatgaca
     3361 aacttctgcc agacgttcgc catcttcaac gacatcccga tcaacaccgc ggcggcaaac
     3421 aacacagccc ccagatacgg actgacgaaa tagccggcac ccatggcggc ggccaggaat
     3481 acaaagacca gaaatgaggt aacggcattc ataggcatga gtccatgtca gcagatgcga
     3541 tgcgataagg gtgcgcgccc gatacaactc gtgtcagtgc gctgtcgcac catcgcaatg
     3601 cagaatgatc gggcactacg acgtcgcgtc cgggctcagt tggccggcgt gtgcggcaac
     3661 gcggcgcccg attcgggaga agtcactgtc ccagagtggc ccggccgcgg cgaatgctct
     3721 atgagccact gtccaccaaa gaaaaacgcc atgagcacca gatagaagag cacgtagcgg
     3781 acgaaaccct ggtgatacgt tgagatcccg gtggcgattt ccgctttaga ggccggcgga
     3841 ttggtaacgc ctcttgccgg cagaagtgct gtgagacgtt gcgcatgcat ttcaggattg
     3901 ctgcgtgccg aggagccatc cggtaaggcc acgatcagcg ccatcaggaa ctgtatcgcc
     3961 gcgggacgcg gcaacccggc cagccgtgag gcttcctccc acggatcgac atccagacgt
     4021 gccaacgccg aaagcacgct cagcatgccg ttagtgtcct cgccaatcga cgcgtacagg
     4081 aattcgtcgt actggcttgt ggctgtagga gttgccagag cgcgcgtcac aatgaaatcc
     4141 gtttgcgcat gcagcgctgg tgtgatgtgg tcatcgcggc atcggactat atccccgcaa
     4201 tcaggtgtgc tcagtgcgct ggcgctcata ggcacacaga agctctctca catgtgcgtc
     4261 aacgcaccga cgacctaccc aattgggagc aggctcctgc gctcacaagg agagaccctt
     4321 cgtgagcgaa ggtagcatga tgcagcgcac agagagcgac ggggcgctgt ttgaagaaaa
     4381 cgagcgagcg gaagcgacgc tcaactccat tgccgatgcg gtcatcagca cggacgttcg
     4441 gggctacgtg acctatctca atgtggtcgc cgaacgattg acggggtggt cacgacagga
     4501 ggcggccggc cgcccgctgg aagaagtgct cagaatcgtc gacgacagca cccggaaccc
     4561 ggcacccaat cccgtggcgt tggccatccg agagaacctg tccgtggcac tcgcggcaaa
     4621 ttgcgttctt gtccgacgcg acggaattga agcggccatt gaggattcaa ccgcgcccat
     4681 tcacgaccgc gatggacagg tgaccggtgc ggtcatggtt tttcatgatg tgagccatgc
     4741 gcgttcgatg tcactcaaaa tgtcgcactt ggctcagcat gacagcctga ccgacttacc
     4801 gaacagagtg ctgctcaacg accgcctgaa gtgcgcgatg gcaaacgccc gacgtcacca
     4861 gcaaagactc gcagtgttgt tcctggatct ggatcgcttc aagcaaatca acgactcgct
     4921 gggacatggc gttggagacc gcctactgaa atccgtggcg caacgcctaa gtggctgcat
     4981 ccgccgcgcg gacacgatca gtcggcaggg cggcgatgaa ttcatattcc tgctgtcaga
     5041 gttagctcac gcgcacgatg cggcggtgtg cgcagagaag atcctcgcgg cgctaagcac
     5101 gccccacgcg gtcgaccagc atgccctgta cgtcacggcg agtatcggta tcgccacatt
     5161 cccggacgac ggaaccgacg ccgacacatt gctgaggcat gccgatattg cgatgcacag
     5221 cgccaaggtc gatggccgca acagctatca gttcttccgg cccgaaatga acacgcgcgt
     5281 catcgatcga cagctgctcg agagaagtct gcgcgaggct atcgagcggg atgagttcgt
     5341 attgcactac cagccgatcg tgaacctgca aacgcgtgcc atcgtcgccg tggaagcgct
     5401 cgcgcgttgg cgacatccgc ggcggggtct gttgtctccc gcggagttca tgccgcttgc
     5461 tgaggagtgc ggcgccattg tctcaatcgg cgcgtgggcg cttcgggaag cttgtcgcca
     5521 ggcggttgtt tggcaacggg cgaagctgca gccgatacgc gttgccgtca atgtgtccgc
     5581 tgcggaattg
//
LOCUS       DATMRM010000224         2767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3214133, whole genome shotgun sequence.
ACCESSION   DATMRM010000224 DATMRM010000000
VERSION     DATMRM010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3214133"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VNZ06_06610"
     CDS             <1..409
                     /locus_tag="VNZ06_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HWW20458.1"
                     /translation="GGSATVRGYAYQSIGPVLSGTLTPAGGVELAAGGVEFRQRLSTN
                     WAAAVFADAGAVTPATGLFGGKFNQQKEPGCFETTAAGQSTGRGLCEGIGVGTGVRYY
                     TPIGPVRVDLAFPLVRTPNSGSFQVYIGLGQAF"
     gene            409..>2767
                     /locus_tag="VNZ06_06615"
     CDS             409..>2767
                     /locus_tag="VNZ06_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20459.1"
                     /translation="MSRPLRIALIIAALIIVGLPLLIVGVVLVAGNIDSGRRFIERTT
                     EQLSGGSVQLQGLAGRFPDHLTLAQLQLRDTQGLWLQAREIQLNTSPLDLLSKEGRID
                     LLHAGTLAILRAPDYGAAAPSNSASQGMWFRELRLDRLDVQRLELAAPLTGDPVAMRI
                     QASARLYSLQQVWAQLSAQRLDSVPSVYQAAVQIDAKQLNVLLDLQEDAGGPLTHLAQ
                     VPDLGALDLHLHLHGPRDAVQTNLDLHAGALVASVKGNVNLKASAADLAVDLDSPAIT
                     PLFGVSWERLSLHGAWHGTLAAPSTTGRLEATALNAPDVHARSVRAQLRGESDKLLLD
                     ATVGGFKLDTPVLEMPDSRPIALHAEARLGEKPKPIEFNLANALVNIRGRWNLSTIDG
                     QAVADVADIRPFVAMGGLDLGGRGVLNVKFSATRKLGRLETSADLEVRDGMAPLAQIL
                     RPRAKAQSTLLFRRGSLGFDNTRVDANSAHASMSGNILYGGPLNLSWKATLATLAPLS
                     PQLAGNLAGSGDIRGVSPNLTLDADVSGQLSAHGSPSGPLHLLVHTRDVPQHANGTLD
                     LSGTLDGAPLKLLAQAQAANDGGVSARIERGDWKSLHAEGSMHLDAKGERPEGRFELH
                     VRQLTDLDRLLGAPLQGGVDASVLFDARGAAHGRAHVTLDAKDVGVPAQQIQELQVRG
                     HVDTPFTRPMLALRLTAATQWNERPTHLNLEARGPMANLDLHADATLDAVGDGEGVVD
                     APAKLEAAATLSADQSELRLTALEVDYRKQNLRLRAPCVIGFGDGL"
BASE COUNT          452 a          883 c          966 g          466 t
ORIGIN      
        1 cggtggcagc gccaccgtgc gcggctatgc ctatcagtcg attgggccgg tattgtcggg
       61 gacgctcacc ccggccggcg gcgtggaact ggcggcaggc ggcgttgaat tccgccagcg
      121 cttatccacc aactgggccg cggcggtgtt tgccgatgcg ggagcggtga cgcccgcgac
      181 gggattgttc ggcggcaagt tcaaccagca aaaggaacca ggctgttttg agacgacagc
      241 tgccggccag tcgaccggtc gcggtctgtg cgagggtatc ggcgtcggta ccggagtgcg
      301 ctactacaca ccgatcgggc cggtgcgggt ggacctcgcc ttcccgctcg tgcgcacgcc
      361 caacagcgga tcgtttcagg tgtatatcgg cctggggcag gcgttctgat gtcacgaccg
      421 ctgcgcattg cgctgatcat cgccgcgctc atcatcgtgg gcctgccgct gttgatcgtc
      481 ggtgtcgtac tggtcgcggg caacatcgac agcggacggc gtttcatcga acgcaccacc
      541 gagcagctca gcggtggcag cgtgcagctg caaggactgg ccggccgctt tccggaccat
      601 ctcacactcg cgcagctgca gttgcgcgac acacagggcc tgtggctgca ggcgcgcgag
      661 atccagctca acacctcacc gctggacctg ctgagcaagg aggggcgcat tgatctgctg
      721 catgcgggca cgctggcgat cctgcgcgct ccggactatg gagccgcggc gccctcgaac
      781 agtgcctccc agggcatgtg gttccgggaa ctgcggctcg accgcctcga tgtgcagcgg
      841 ctggaactcg cagcgcctct gaccggcgat ccggtcgcca tgaggatcca ggccagcgca
      901 cgcctgtatt cgctgcaaca ggtgtgggcg cagctgagcg cacagcgcct ggattccgtg
      961 ccctcggtgt atcaggccgc ggtgcagatc gatgcgaagc aactcaatgt gctgctggat
     1021 ctgcaggagg atgccggcgg tccactgacg catctggcgc aggtgccgga tctgggcgcg
     1081 ctcgatctgc atctgcatct gcacggtccg cgcgacgcgg tgcagaccaa tctggatctg
     1141 catgccggcg cgctggtggc ctccgtcaag ggcaacgtga atctgaaggc gagcgctgcg
     1201 gatctggcgg tcgatctgga ttcgccggcg atcacacccc tgtttggtgt ctcgtgggag
     1261 cggctatcac tgcacggtgc ctggcatggc acgctggccg cgccctcgac caccgggcgc
     1321 ctcgaagcca cggcgctcaa tgcgccggat gtgcacgccc gatcggtgcg cgcgcagctg
     1381 cgcggtgagt cggacaagct gctgctggat gccactgtcg gcggtttcaa actcgacacg
     1441 ccggtactgg agatgcccga ctcgcggccc atcgcgctgc atgccgaagc gcggcttggc
     1501 gagaaaccca aaccgatcga gttcaatctt gcgaatgcgc tggtgaacat ccgcgggcgc
     1561 tggaatctat caaccatcga cggtcaggcg gtcgcggacg tggccgatat ccggccgttc
     1621 gtcgcgatgg gtggactcga cctcggtggg cgcggcgtcc tcaacgtcaa attcagcgcc
     1681 accaggaaac tcgggcgcct ggaaaccagc gccgatctgg aggtgcgcga cggcatggcg
     1741 ccactggcgc agattctacg gccgcgcgcg aaagcgcaga gcacgctgct gtttcggcgc
     1801 ggcagtctcg ggttcgacaa cacgcgcgtc gatgcgaaca gcgcacacgc ttcgatgagc
     1861 ggcaacattc tctacggcgg gccgctcaac ctgagctgga aggcaacgct tgcaaccctc
     1921 gcgccgctgt cgccgcagct ggctggcaat cttgcgggct cgggcgatat ccgcggcgta
     1981 tcgcctaatc tcaccctgga tgcggatgtc agcggacagc tctcggcgca cggctcgccc
     2041 tccggtccgc tgcatctgct ggtgcatacc cgggatgtcc cgcagcacgc caacggtacg
     2101 ctcgacctga gcggcacgct ggacggcgca ccgttgaagc tgctggcgca ggcgcaggcc
     2161 gcgaacgatg gcggggtgtc cgcccggatc gagcgcggcg actggaagag cctgcatgcg
     2221 gaaggctcga tgcatctgga tgcgaagggt gagcgcccgg aaggcaggtt cgagctgcat
     2281 gtgcggcagc tcaccgatct cgaccggctg ctcggtgcgc cgctgcaggg cggcgtcgat
     2341 gccagtgtgc tgttcgacgc gcgcggtgcg gctcacggcc gcgcccacgt gacgctcgac
     2401 gcgaaggacg tgggcgttcc ggcgcagcag attcaggagc tgcaggtgcg tggacacgtt
     2461 gatacgccgt tcacccggcc gatgctggcg ctgcgcctga cggccgcaac gcaatggaat
     2521 gagcggccga cgcatctgaa tctggaagca cgcggaccga tggcgaatct ggatctgcac
     2581 gccgacgcga cgctcgatgc ggtcggagac ggcgagggcg tggtcgatgc gccggcgaag
     2641 ctcgaggcgg cggcgacgct cagcgccgat cagtccgaat tgcggctcac ggcactggaa
     2701 gtggattatc gcaaacagaa tctgcggctg cgggccccgt gcgtgatcgg tttcggcgac
     2761 ggcctgt
//
LOCUS       DATMRM010000225         4330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1061661, whole genome shotgun sequence.
ACCESSION   DATMRM010000225 DATMRM010000000
VERSION     DATMRM010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061661"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            592..1161
                     /locus_tag="VNZ06_06620"
     CDS             592..1161
                     /locus_tag="VNZ06_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20460.1"
                     /translation="MSARDPNFYHLLLRIDQDYAEQTRAQRCRCGGVLHSARYPRKPR
                     GISFEARCHCEFRYSFCCADCRRRTTPFSVRFLGRRVYTAAIMIVVSATRASAGTAAA
                     MRQLDALGVPPSTIARWRHWWMTGFVGTPFWALGRAAFVPPVETAQVPASLLGRFATT
                     QPAEPLPCLLRWLSPLTSGSAVSTLREGR"
     gene            1260..2696
                     /locus_tag="VNZ06_06625"
     CDS             1260..2696
                     /locus_tag="VNZ06_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HWW20461.1"
                     /translation="MTELHDLPLRDRWARLRFAVVGPLLAAPPEPGQVRELITALAAK
                     RWRHPVTGDDVQFGRSTVERWFYRARKAAHDPVASLRDRKRPSGAGASLSPQAVQAIR
                     AQYREHPGWTCQLHFDNLKVTLGPANLPSYTTVRRYLRAQGLYRERKPRHTSTGAMLA
                     RDRLEKLEVRSFEVEHVNALWHLDFHHGSRKLLTHDGQWITPMLLCVLDDRSRVVCHA
                     QWFLDETARSLIHGLTQALQRRGRPRALMTDNGAAMLAEETTAGLLALGVLHQTTLPY
                     SPYQNAKQETFWARIEGRVMAMLEGEPNLTLELLNQATHAWIEREYHHAHHSEIGGTP
                     LKRYLAGPDVGRECPSSDALRAAFRIDVTRAQRRSDGTVSLAGTRFEIPSAYRHLQRV
                     HLRYARWDLSGVDLVDADSGAVLCPLRPLDKAANADGQRRRLDAVNVEPPPASAPGIA
                     PLLRQMLADHAATGLPPAWIPANWQEEA"
     gene            2693..3550
                     /locus_tag="VNZ06_06630"
     CDS             2693..3550
                     /locus_tag="VNZ06_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW20462.1"
                     /translation="MSQRLQALYGLKWNPFSPELPLEALYVPPRVENFCWRIENALVR
                     EGGFAMIHGEPGTGKSVVMRVLAEKLERLTDLTVVSINHPQSNLADFYREMGDIFGLE
                     LRPHNRWGGFRSLRDRWMSHLQSTRRRPILLIDEAQEMSPGVLNELRLMASARFDSQP
                     LLCVVLAGDTRLTDKLRRDELLPLGSRIRSRLGTEKASADDLLACLDHLLASAGAPQL
                     MTPPLRHTLCEHALGNYRVLTTLANELLTTAAQRELSELDEKLYFEVFAPSTQSSRRT
                     PARQPNGAR"
     gene            3552..3767
                     /locus_tag="VNZ06_06635"
     CDS             3552..3767
                     /locus_tag="VNZ06_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20463.1"
                     /translation="MSRKASPHKPNASRHLPLPELSDEAAYMMQFFIEDLYLWFSRAY
                     ATQIRRYREPWYRGPTQPPHQSIDEPF"
     gene            3665..>4330
                     /locus_tag="VNZ06_06640"
     CDS             3665..>4330
                     /locus_tag="VNZ06_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWW20464.1"
                     /translation="MVQPRLRHPDPPLSRALVPRSDTAATPEHRRAILKRQRELHRSR
                     FTSSLEHHLCCSLPHRFTCSRSLLYEKRNATAYVTLNRPQVMNALNQQAIAELRAAFE
                     DAQDDPQIRGVIITGAGNRAFIAGADINELAKATPIEAERQTRAGQALLDLVEHLGKP
                     VIAAVNGLALGGGCETALACTIRLASPGAKFGQPEIKLGLIPGFGGTQRLPRLVGKGV
                     ALQL"
BASE COUNT          799 a         1481 c         1281 g          769 t
ORIGIN      
        1 ttcaggccta gcaatttgtt ccagtaacac cgaatggagc attctgcatt acatgaatat
       61 gtaatttcgc cgcaaaggac aacgatgtaa attgagtaat aagctcatcg ccaaactgaa
      121 ttgatgaaat gcatgactcg acggattccc gtgctttcgg accagcgcat agccgccatg
      181 ggactcaacg taaacagcgg cgacttgccg atgctctatt accccgaggt cattgccgac
      241 ttttttgcga agtcagctaa ctcgctcgca gcctgaactg gtatagggaa cggcagaggt
      301 cgataggtcg aacttcacgc tgatcactac tgggatgaac gcgcaacacc cgaccggtaa
      361 ggcgccggac gtccatgaac gcggaaggat caatctgatg tcacacaatt tgtctgttcc
      421 ctccttcgac acccttctct gagcgtgagc acgtaaatcg atgggaattc ggggcgtttt
      481 gagcagcttg cgtgatgtag cggagggggt ctccctcagg catcttgcac aaacgacgcc
      541 caagggagag tctcgagtct gccaagaacc gagacttcca gagtgtgcca cgtgagtgcc
      601 cgcgatccca acttctatca cctgctgttg cgtatcgatc aggactacgc cgaacagacc
      661 cgcgcgcagc gttgtcgctg cggtggcgta ctccatagcg cccgctaccc gcgcaagccg
      721 cgcggaattt cattcgaagc ccgctgccac tgcgagttcc gctacagctt ctgctgcgcc
      781 gactgtcgcc gtcgtaccac gccattctcg gtgcgctttc tcggccggcg cgtctacacc
      841 gccgcaatca tgattgtcgt cagcgccacg cgtgcgagcg ccggtacggc cgcggcaatg
      901 cgccagctcg atgcgctggg cgtgccgccc tcgacgatcg cgcgatggcg gcactggtgg
      961 atgaccgggt ttgtcggcac accgttctgg gcgctcggac gggcggcgtt cgtgccgccc
     1021 gtcgagaccg cacaggtgcc ggcaagcctg ctcgggcgct tcgctaccac gcagcctgcc
     1081 gagccattgc catgcctgct gcgctggctc tcaccgctca ccagcggcag cgccgtgagc
     1141 accctgcgtg agggacggta accgcccgca gaacttgtcg ctcgcccctc tcggactgcg
     1201 ccggtagtct cgcctctccc ccgaatcgcg gcgcggtgcg ccgcatccct ggagagagca
     1261 tgactgaact tcacgattta cccctgcggg atcgttgggc gcgcctgcgc tttgcggtcg
     1321 tgggcccctt gctggcagca ccgcctgaac ccgggcaggt gcgcgaactc atcaccgcgc
     1381 tggccgccaa acgctggcgt catcccgtca cgggcgacga tgtccagttc ggccgctcca
     1441 ccgttgagcg gtggttctac cgggcccgca aggcggccca cgatccggtt gcgagcctgc
     1501 gcgaccgcaa gcgccccagc ggcgccggcg caagcctgtc gcctcaggcg gtacaggcga
     1561 ttcgcgcgca gtaccgggaa catcccggct ggacctgcca gttgcacttc gacaatctga
     1621 aggtcacgct cggcccggcg aacctgccgt cgtacacgac ggtgcgtcgt tatctgcgtg
     1681 cgcagggcct gtaccgtgaa cgcaagccgc gtcataccag caccggcgcg atgctcgcgc
     1741 gcgatcgcct cgagaagctc gaggtccgca gcttcgaggt cgagcacgtc aacgcgctct
     1801 ggcatctcga tttccatcac ggctcgcgca agctgctcac gcatgatggc cagtggatca
     1861 cgccgatgct gctgtgcgtg ctcgatgacc gttcgcgcgt cgtctgtcac gcgcaatggt
     1921 ttctcgatga gaccgcacgt agtctgatcc atgggctcac ccaggcgctc cagcgtcgtg
     1981 gccggcctcg cgcactgatg accgacaacg gcgcagcaat gcttgccgag gagacgaccg
     2041 ccggcttgct cgcgctcggc gtgctgcacc agacgacctt gccatattcg ccgtaccaga
     2101 acgcgaagca ggagacgttc tgggcccgca tcgaagggcg cgtgatggcc atgctcgaag
     2161 gcgagcccaa tctcacgctc gaactgctca atcaggccac ccacgcgtgg atcgagcgcg
     2221 agtaccacca cgcgcatcat tccgagatcg gtggcacgcc gctcaaacgc tatctggccg
     2281 gccccgatgt gggccgcgaa tgcccgtcga gtgacgcgct gcgcgccgca ttccgtatcg
     2341 acgtgacgcg cgcccagcgg cgctcggatg gcaccgtgag tctggccggc acgcgcttcg
     2401 agattccctc ggcctaccgg cacctgcagc gcgtgcacct gcgctacgcg cgctgggacc
     2461 tgagcggcgt cgatctcgtc gacgccgata gcggcgccgt gctgtgcccg ctgcggccgc
     2521 tggacaaggc cgccaatgct gatggccagc gccggcgact cgatgccgtc aatgtcgagc
     2581 cgccaccggc cagtgcgccc ggcatcgcgc cgctgctacg ccagatgctg gccgaccacg
     2641 ccgcaaccgg gttgccgcca gcctggattc ccgccaactg gcaggaggag gcatgagcca
     2701 gcgtctgcaa gccctctacg gcctgaagtg gaacccgttc tcgccggagc ttcccctcga
     2761 ggcgctgtac gtgccgccgc gcgtcgagaa cttctgctgg cgcatcgaga acgcgctcgt
     2821 gcgcgaaggc ggcttcgcga tgatccacgg cgagccgggt accggcaaga gcgtggtcat
     2881 gcgcgtgctg gccgagaagc tcgagcgact gaccgatctg acagtcgtct cgatcaatca
     2941 tccgcagagc aacctcgccg acttctatcg cgagatgggc gacatcttcg gtctggaact
     3001 ccggccgcac aaccgctggg gcggcttcag gtcgctgcgc gaccggtgga tgtcgcacct
     3061 gcaaagcacg cgccggcgcc caatcctatt gatcgacgaa gcgcaggaaa tgagtcccgg
     3121 cgtgctcaac gaactgcgac tcatggccag cgcccgcttc gattcgcagc ccctgctgtg
     3181 tgtggtgctc gccggcgaca cgcgcctgac cgacaagctg cggcgcgacg aactgctgcc
     3241 actgggcagc cgtatccgtt cccgcctggg gaccgagaag gcttcggccg acgacctgct
     3301 ggcctgcctc gatcatctgc tcgccagcgc cggcgccccg cagctgatga caccgccgct
     3361 gcgccacacg ctgtgcgaac acgcgctggg caactaccgc gtgctcacca cgctcgccaa
     3421 cgaactgctc accaccgccg cgcagcgcga actgtccgaa ctcgacgaga agctgtactt
     3481 cgaggtcttt gcaccatcaa cacagtcctc gcgccgcacg ccagcgcgtc aacccaacgg
     3541 agcccgatga catgtcccgt aaagcatcac ctcacaaacc caacgcctcc cggcacctgc
     3601 cattgcccga gctctccgac gaggcagcct acatgatgca gttcttcatc gaagacctct
     3661 atctgtggtt cagccgcgcc tacgccaccc agatccgccg ctatcgcgag ccctggtacc
     3721 gcggtccgac acagccgcca caccagagca tcgacgagcc attctgaaac gacaacggga
     3781 gcttcatcgc tcccgtttta cttcgtccct cgaacatcat ttatgttgct cattgcccca
     3841 tcgatttacg tgctcacgct cacttctcta tgaaaagaga aatgcaacag cctatgtgac
     3901 gttgaatcgt ccccaggtga tgaatgcgct gaaccagcag gcgatcgcag aactacgcgc
     3961 ggcgttcgaa gatgcgcaag acgacccaca gattcgcggc gtcatcataa ccggcgcagg
     4021 caatcgggca ttcatcgccg gtgccgatat caacgagttg gccaaggcaa cgccaattga
     4081 ggccgaacgc cagacacgtg cgggtcaagc acttctcgat cttgtcgaac atctcggcaa
     4141 gccagtcatt gctgcagtga acggcctggc acttggcggc ggatgtgaaa ccgcgctcgc
     4201 ctgcacgatc cggctagcca gtccgggcgc gaagttcggg cagcctgaaa tcaagctggg
     4261 actcatccct ggctttggcg gaacgcaacg actccctcgc ctcgtcggca aaggtgtcgc
     4321 gttgcagctc
//
LOCUS       DATMRM010000226         4173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1062070, whole genome shotgun sequence.
ACCESSION   DATMRM010000226 DATMRM010000000
VERSION     DATMRM010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062070"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VNZ06_06645"
     CDS             complement(<1..799)
                     /locus_tag="VNZ06_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20465.1"
                     /translation="MQIQDRLVRSNAIQSFLAAATDAEIVWQLTLVGVVIVLGMLASG
                     YARRWRARHLAAMPLRGLRSRLIEISAIEAPFVLCLIVLLVGRTVVAAIGSPLTLLDV
                     ALQLVTALILVRLALYLLRLALGPRSWLRTWETRLTLLVWLGISFELVGWFGEIEKTL
                     DEINLLPGAGRFTLWALLKGLVIITAFVLVTSVIARGIERRVMHIEAIALSTRIGITK
                     FTYFFLVGLGVLLGINAAGVDLTALTVLTGAIGLGLGFGLQSIASNFV"
     gene            complement(821..1045)
                     /locus_tag="VNZ06_06650"
     CDS             complement(821..1045)
                     /locus_tag="VNZ06_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HWW20466.1"
                     /translation="MTDQEDNVDRDLFRNVEKLRQLRIEHRDLDEIISRLSMDIHIDE
                     LQLKRLKKRKLALKDQIMKLESQLIPDLNA"
     gene            complement(1048..1896)
                     /locus_tag="VNZ06_06655"
     CDS             complement(1048..1896)
                     /locus_tag="VNZ06_06655"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWW20467.1"
                     /translation="MLSRIDLHTHSDRSDGALAPAALVARAAARQVALLALTDHDTLA
                     GCSAARAACEQHGIEFLSGLELTCEWRGREVHVVGLAVNEADSSLRDHCCSVLTMRRE
                     RIHEIACRLSAAGLPGEALAEQALAVPAPTRTHIARAMCAAGLATSIQRAFDRWLKRG
                     QPGYVAQKWPELATVVRCVLGAGGIAVLAHPHRYKVSNGVLRELVAEFRDAGGGGIEV
                     SLPGMEPADTDRAATLARRFDLAGSVGSDFHEPDLPWRPLGRLDKLPEGVRPITAHLG
                     PVNGRS"
     gene            complement(1897..2706)
                     /gene="cysQ"
                     /locus_tag="VNZ06_06660"
     CDS             complement(1897..2706)
                     /gene="cysQ"
                     /locus_tag="VNZ06_06660"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HWW20468.1"
                     /translation="MTPTELAAVLSAVLDIAHDAGREIMQIYEGEFSVTLKSDRSPLT
                     EADLAAQRLIAARLSALRPDIPLLGEESGDQAFAQRRAWRTLWLIDPLDGTREFVKRN
                     GEFTVNIALVKDGEPILGVVHAPARNVSYAGARGCGAFRVGADAVRTSIHVQSTAPTR
                     LRVLGSRSHGDARLDHALERLGPQERLSIGSALKFGLLAEGAGDLYVRRGATSEWDTA
                     AGQAVVLEAGGCVVDPHGQPLRYNQRDTLINPWFIAYADRSRDWIGALGPL"
     gene            2729..3397
                     /gene="yrfG"
                     /locus_tag="VNZ06_06665"
     CDS             2729..3397
                     /gene="yrfG"
                     /locus_tag="VNZ06_06665"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF011564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HWW20469.1"
                     /translation="MTAPALSLAQWNQIDTVLLDMDGTILDLAFDNHFWRTHVPQAWA
                     LQCQLSEQEALRQLERRFRACEGTLDWYCLDYWSRELELDLSALKKKHSDRIRWLPGA
                     REFLLRVRALGKRLVLITNSHPTTLAIKDQHTQVISHFDAGFSSHHFGAPKEQMRFWQ
                     ELAKVERFDPQRSLFVDDSLPVLRAARAAGIQLVYAVNRPDSDAPPRPQQEFPFVDAV
                     AELL"
     gene            complement(3414..>4173)
                     /locus_tag="VNZ06_06670"
     CDS             complement(3414..>4173)
                     /locus_tag="VNZ06_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWW20470.1"
                     /translation="RLPRPEHRQSMLFSATLSQRVLELAYEHMNNPELVRIEPDRVTI
                     ERVRQVIYFPSMEEKVPLLIGLLRDTEAHRTMVFVNMRRTAERLESAVRANGLNAQAL
                     SGDVPQKKRLQFLRQFHEGELAVLIATDVASRGLHIPDVSHVFNFDLPQDPADYVHRI
                     GRTARAGADGDAISFGCEEYAMGLPDIETYIGHKIPVATVTPELLAQLTADTYNAPDQ
                     RYSAPMRRGSRPGGGHRGGRPGNRQGGKGRRSGR"
BASE COUNT          757 a         1348 c         1349 g          719 t
ORIGIN      
        1 tgacgaaatt gctggcaatc gactgcagac cgaaccccag ccccaggccg atcgcgccgg
       61 tcagcacggt gagcgcggtc aggtccacgc cggcggcgtt gataccgagc aggaccccca
      121 gtcctaccag gaagaaatag gtgaatttcg tgatgccgat gcgggtggac agcgcgatcg
      181 cctcgatgtg catcacgcga cgctcgatcc cacgcgcgat cacgctggtg accagaacga
      241 aggccgtgat gatcaccagt cccttgagca gcgcccacag cgtgaagcgt ccggcgccgg
      301 gtagcagatt gatctcgtcg agcgtcttct cgatctcgcc gaaccaaccg accagttcga
      361 aggaaatccc cagccacacc agcagcgtga gtcgggtctc ccaggtgcgc agccatgagc
      421 gcggtccgag cgcaagacgc agcaggtaca acgcgaggcg caccaggatc agagccgtga
      481 cgagttgcag ggccacgtcc agcagcgtca gcggggatcc gatcgccgcg accacggtac
      541 gtccgaccag cagcacgatc aggcaaagca cgaacggggc ttcgatggcg gagatttcga
      601 tcaggcgcga gcgcaggccg cgcagcggca ttgccgcaag atgtcgggcg cgccagcgcc
      661 gcgcatagcc cgaggcaagc atgccgagga cgatgacgac gccgaccagc gtcagctgcc
      721 agacgatctc ggcgtcagta gcggcggcga gaaacgattg aatagcgttg ctgcgaacga
      781 gcctgtcttg gatttgcatg ccgccgatgg tccggcttct ttaagcgttg agatccggga
      841 tcagctgact ctcgagtttc atgatctgat cctttagcgc gagcttgcgc ttcttgaggc
      901 gtttgagctg cagttcgtca atatggatgt ccatcgatag tcgcgagatg atttcatcca
      961 ggtcgcgatg ttcgatgcgc agctgccgca gcttttcgac gttgcggaac aggtcgcggt
     1021 cgacgttatc ttcctgatca gtcacgctta agaccgccca ttgaccggcc caaggtgcgc
     1081 cgtgatcggc cgcaccccct cgggtagctt atctaagcga cctaacggac gccacggcag
     1141 gtccggctcg tgaaaatccg agcccacgga accggcgaga tcgaagcggc gtgcgagcgt
     1201 tgcagcgcga tcagtatccg ccggctccat gcccggcaga ctgacttcga tgccgccgcc
     1261 gcccgcgtcg cggaactctg cgaccagttc ccgcagcaca ccgttggaga ccttatagcg
     1321 gtgcgggtgc gccagcaccg cgataccgcc ggctccgagc acgcagcgca cgacggtcgc
     1381 cagctccggc cacttctgcg ccacataacc gggttgacca cgcttgagcc atcgatcgaa
     1441 cgcgcgttgg atgctcgttg ccaggcctgc agcgcacatg gcgcgcgcga tgtgtgtccg
     1501 ggttggagcc gggacggcca gtgcctgctc ggccagcgcc tcgccgggca gacccgctgc
     1561 gctcaaacgg catgcgattt catgaatgcg ctcgcgtcgc attgtgagca ccgaacaaca
     1621 atgatcccgc aatgaggagt cggcctcgtt caccgccagg ccgaccacgt gaacttcgcg
     1681 gccgcgccat tcgcaggtga gctcgaggcc ggatagaaac tcgatgccat gttgttcgca
     1741 ggcggcgcgt gcagcggagc agccggccag ggtgtcatgg tcggtcagtg ccagcagcgc
     1801 cacctggcgt gcggcagcgc gcgccaccag tgctgcgggt gccagcgcgc catccgagcg
     1861 atcgctgtgg gtgtgcaggt cgatgcgcga gagcatttac agagggccca atgcaccgat
     1921 ccagtcacgg ctgcgatcgg cataggcgat gaaccagggg ttgatgaggg tatcgcgctg
     1981 gttgtagcgc agaggctgcc catggggatc aaccacgcag ccgccggctt ccagcaccac
     2041 ggcctgaccg gcggcagtgt cccactccga ggtcgcgcca cgccgcacgt acagatcgcc
     2101 ggcgccttcg gccaacaatc cgaacttcag ggcactgccg atgctgaggc gttcctgggg
     2161 cccgagacgc tcgagcgcgt gatcgagcct ggcatccccg tgggaacggc taccgagcac
     2221 gcgcaggcgt gtcggggccg ttgactgcac atgaatcgat gtgcgcacgg catccgcgcc
     2281 gacccggaat gcgccgcaac ctcgggcccc ggcatagctg acgttgcggg ccggcgcatg
     2341 caccacaccc agaatcggtt cgccgtcctt caccagtgca atgttgaccg tgaactcgcc
     2401 attgcgtttg acgaactcgc gcgtcccgtc caggggatcg atcagccaca gcgtgcgcca
     2461 tgcgcggcgc tgcgcgaagg cctggtcacc ggactcttcg cccagcaatg ggatgtcggg
     2521 ccgcagtgca ctcagacgcg cggcgatcag gcgctgcgcg gccagatcgg cttcagtgag
     2581 gggcgaacgg tcggacttca gggtcacact gaactcgccc tcgtagatct gcatgatttc
     2641 gcgtccggcg tcgtgcgcga tgtcaagcac cgcagacaac accgccgcca actccgtggg
     2701 tgtcatacgc tctatgatat ccgacaacat gactgcccca gccctgagtc tcgcgcagtg
     2761 gaatcaaatc gataccgtcc tgctggacat ggacgggacc atcctcgacc tggccttcga
     2821 caatcatttc tggcgcaccc atgtgccgca ggcctgggcg ttgcagtgcc agctcagcga
     2881 gcaggaggcg ctgcggcagc tggaacgtcg ttttcgggcg tgtgagggca cgctcgactg
     2941 gtattgcctt gattattgga gccgagagct tgaattggac ctttccgccc tcaaaaagaa
     3001 gcattccgac aggatccgat ggctgcctgg cgcgcgcgag ttcctcttgc gggtgcgcgc
     3061 gctgggcaag cgtttggtgc tgatcaccaa ctcgcacccg acgacgcttg ctatcaagga
     3121 ccagcatacg caggtcatca gccacttcga tgccggcttc agctcccatc attttggtgc
     3181 gccgaaggag cagatgcggt tctggcagga gcttgcgaag gtcgaacgct tcgacccgca
     3241 gcgcagtctg ttcgtcgatg acagtctgcc ggtgttgcgc gccgcccgcg ccgcaggcat
     3301 ccaactcgtt tatgcggtga atcggccgga cagcgatgca ccgccgcgcc cgcagcagga
     3361 atttccgttc gtcgacgcgg tcgccgagct gctgtagcgc ggcccaggca atctcagcga
     3421 ccggaacgcc gtccttttcc gccctggcgg ttgccagggc gaccgccgcg gtgcccgccg
     3481 cccggacgac tgccgcggcg catcggagcg gaatatcgct gatcgggagc gttgtaggtg
     3541 tccgcagtga gctgcgccag caattccggg gttaccgtgg ccaccggtat cttgtggccg
     3601 atataggtct cgatgtccgg caggcccatc gcgtactcct cgcagccgaa gctgatggcg
     3661 tcgccgtcag cgccggcgcg cgcagtgcgg ccgatgcgat gcacgtagtc cgccgggtcc
     3721 tgcggcaggt cgaagttgaa cacgtggctg acgtccggga tgtgcagtcc gcgcgaggcg
     3781 acgtcggtgg cgatcagtac cgcgagctca ccttcgtgaa attgccgcag gaactgcaga
     3841 cgcttcttct gcgggacgtc ccccgaaagc gcctgcgcgt tcaggccatt ggctcgcacc
     3901 gccgactcga gccgctcggc ggtgcggcgc atgttgacga ataccatcgt gcggtgcgcc
     3961 tcggtgtcac gcagcagacc gatcagcagc ggcaccttct cctccatcga ggggaagtag
     4021 atcacctgtc ggacgcgttc gatcgtgacc cgatcgggct cgatccgcac cagctcggga
     4081 ttgttcatgt gctcgtacgc gagttcgagc acccgctgcg acagcgttgc cgagaacagc
     4141 atcgactggc gatgctcagg acggggcagg cgg
//
LOCUS       DATMRM010000227         8093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3844928, whole genome shotgun sequence.
ACCESSION   DATMRM010000227 DATMRM010000000
VERSION     DATMRM010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3844928"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..515)
                     /locus_tag="VNZ06_06675"
     CDS             complement(150..515)
                     /locus_tag="VNZ06_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HWW20471.1"
                     /translation="MFKFSMGYLPVLAAGLLAVTATASATAGILDRGDQPPSVTVRYD
                     DLNLSTRDGRTVLYRRLQRAAGQVCPSVDSADLGQIARAQHCQVAAVQRAVRQIGGPM
                     VAQLQAEHGLAQERSVSQN"
     gene            783..1901
                     /locus_tag="VNZ06_06680"
     CDS             783..1901
                     /locus_tag="VNZ06_06680"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWW20472.1"
                     /translation="MTERGLNMLSLLGVLSVYWCYVALSNVLYAHSMQATIEDHWAFA
                     PPLPRLLQHAFLYPLLMGSVWISLRVGWRPVWRAVPVQVLMGLGFAVLSRPALIVGEI
                     IDGSMGHTWAKVFAERASLISGTGLATWAASTLSFLLTYGFGLALVTGLSWYQRYRDS
                     QERFDALERSLSATRLAALRMQLSPHTLFNLLHTIRGQIAWDPSSAQTMVVQLGDVLR
                     RLLAAGERDFSRLADELEFVRLYLELQRSRFSDRLSFHISELGELNRLWVPSLILQPL
                     VENAVAHGLSGHAGAVHVSVEASSENGTLKLAVTNTTASDTVAGAQGIGLRNVRERLV
                     LHFPDSATFRASPADAQTWVAEIQMPLVLDARGNRSAP"
     gene            1898..2698
                     /locus_tag="VNZ06_06685"
     CDS             1898..2698
                     /locus_tag="VNZ06_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HWW20473.1"
                     /translation="MNAAAVMDVVIVDDEPSARRTLRECCAREPDLRVVGEFGESAGA
                     LEAIRAQRPQLLFLDIQIDALSGIQIARSLDPSHLPLIVFVTAYDSYALEAFEVSAVD
                     YLLKPFDEERFRKTLARVRRRREAESVADRNQALSQVLAQIERSARASADSRPRILAE
                     SGSRMHMLDIAQVEVAEADRNYVKLIVGREVYLTRSTLQQAEKALQSQSTLRISRSCI
                     VNVTHVREVSRTPRGDFILVLAGGTTVTSSEGYRDVVRDYLAQLKLGA"
     gene            complement(2668..3585)
                     /locus_tag="VNZ06_06690"
     CDS             complement(2668..3585)
                     /locus_tag="VNZ06_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWW20474.1"
                     /translation="MATLSYRTIHANGIDFHVAVEGLGSRLALCLHGFPEVSFSWRHQ
                     IPLLASLGFQVWAPDLRGYGGTARPLGVDAYAIERLEEDVAGLIEASGCREVLLIGHD
                     WGAAIAWNYAMFGRLPISRLIIMNVPHPALFAKGLRTLQQMRKSWYILFFQLPAIPEW
                     ILSRDHCAAIGHALRGMAVDKSRFPDEVLRVYRDNAAQPGALTAMINYYRALVRGRRR
                     LGARRITTINVPTLLLWGEVDTALGKELTYGTDHYAPQLTLRYLPNVSHWVQQEAPET
                     VNAMLQAWLSGNEVPQAPGAQAPSLSCAR"
     gene            3601..4746
                     /locus_tag="VNZ06_06695"
     CDS             3601..4746
                     /locus_tag="VNZ06_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWW20475.1"
                     /translation="MKYLPLVWNGLWRRRAQTLFTLASIAVAFILFGILAGIDAGFAH
                     TLAASRMDRLFTDPRFGAPMPMSYGEQIARVPGVSVVAPRSLLFGYFQDPKNTLGVLC
                     SDRRFFALRPEISISAPQIELLRQMRTGAAVSVELASKFGWKVGDKVPLQTNTAQADG
                     SRVWTFDIVAIVDDLNYPGQGGWFIANYQYLDEGRVDGKSTIDRFLVRISDPRRGAQI
                     GREIDQRFANSAAPTRTNSERSQAQSGLQFIGDVNFLTDAVLGAVFFMLLFLSGNTLL
                     QSVRERTAEFAMLKALGFSNVAVLSLVIAESVLLCLLAATAGLVMSELLIPIAHDALA
                     NFVVLLQMPWRAMLRGFALALIVALLGSLLPAWRVMQLNVVNALVRR"
     gene            4806..5933
                     /locus_tag="VNZ06_06700"
     CDS             4806..5933
                     /locus_tag="VNZ06_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWW20476.1"
                     /translation="MRAIPQRKGASLVIVIGMALVVAVTLSILSMSTGFMRSVRNTGR
                     EDRAIVLSQGSQFEFASALSRDNVTTICDAPGIRTASDGKPIVSAESLATVAVVKKSD
                     GLDAFVALRGIGPQGLALRPEIKLVAGRLFRPAKHELIVGKSARAQFAGLDLGSQVPL
                     PQGDWTVTGIFESNGDGHESELLADSETVFAAMRASTFHSVTVMLERAQSFEQFRTAL
                     TTNPTLTVDVVREPQYLAAQSEFLNDFLSMIAYVVGGIMGLGAAFGALNTMYSVVSAR
                     AIEIATLRAIGFGGAAVVASVLVESLLLALAGAAIGASAAWIAFNGDLHVAGGLVFKL
                     AVTPGLVGLGIGFAGTLGLIGGLFPAIRAARLPVVTALNNQ"
     gene            complement(5963..7027)
                     /locus_tag="VNZ06_06705"
     CDS             complement(5963..7027)
                     /locus_tag="VNZ06_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HWW20477.1"
                     /translation="MAIVAVMLLSLGLALNYNSPCRPADVASASKTLMKAITQRCYGS
                     PDVLKLEDIEKPTPADNEVLVRIHAASVNPIDWHLMRGTPAVVRLQTGLGRPRYSGAG
                     IDFAGTVEAVGTHVKQFQPGDSVFGGTDGAFAEYVRMPADGALVMKPKNLTFEQAAAA
                     PIAAITALQALRDRGRIQPGQKVLINGASGGVGTFAVQIAKALGAEVTGVCSTRNVEM
                     VRSIGADHVIDYTREDFTRGAQRYDLILDNVGNRSVLEYRRVLAPAGIIVIVGFDSIS
                     GIARTMVLSPFVSQKVVSFQANLNKADLNVLRELMQDAKVTPVIDSRYRLSELPAAIG
                     YLEKGHARGKVVITMEQPGG"
     gene            complement(7160..7549)
                     /locus_tag="VNZ06_06710"
     CDS             complement(7160..7549)
                     /locus_tag="VNZ06_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWW20478.1"
                     /translation="MSAIMALGAMFGAANTMYSAVSARSTEIATLRAIGFGPSAVVAS
                     LLIEALLLAVIGAALGALVVGLLFNGSTIGAHLGFESRRVLTKLHVDLSLIALGMGWA
                     CGIGLFGAILPAVRAARLPVVEAMRDA"
     gene            complement(7718..>8093)
                     /locus_tag="VNZ06_06715"
     CDS             complement(7718..>8093)
                     /locus_tag="VNZ06_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20479.1"
                     /translation="QGAPGVHQGPDGEPIVSAEVLVPLRVREYSKDAGAEVSVRGIAP
                     RAFLLRPEIKIIAGRVMQPGVHELLVGRGASSQFKGLRLGEQPSIHGVPWTVVGIFES
                     NGDTHESELLAGCRNALIRDSS"
BASE COUNT         1417 a         2542 c         2605 g         1529 t
ORIGIN      
        1 tggcatcgcc ggaggactcc gatgaggcac ccaggcacaa cgtcacggct gcaaccagta
       61 cacccaacaa tacgagatga cgcattggac ttaccccctt gggttctttg tgaggttccc
      121 tgacagcgcg cgattgtagt ccgagtacat caattctgag agacgctgcg ttcctgagcg
      181 agaccgtgct cagcctgcag ctgagcgacc atcggaccgc cgatctggcg cactgcacgc
      241 tgtactgccg cgacctgaca gtgctgcgcc cgtgcgattt gacccagatc ggcggaatcc
      301 acactcgggc atacctgccc ggccgcccgt tgcagacgcc gatagaggac ggttcggccg
      361 tcgcgggtgg acaaattcaa atcgtcgtaa cgcactgtga ccgagggagg ctgatcgccc
      421 cggtcgagga tgccggccgt tgcgctcgcg gtagcggtga cagccagcaa gccggccgcc
      481 aggaccggca gataacccat gctgaacttg aacatatgcg gctccttgag tttgcgaacg
      541 atcgccatct acttgggcga ccggcatata agacgccgcg gtagggcggt tggtcgcttg
      601 tgccgcaacg cactcgtccc tgggggcaac gggtcggtcc caaaccggga gcccggacac
      661 tgcgggcagc ggggggtggt gtaggcttgg gtcagagaat gtcgatgcca ccgggcccgt
      721 cagagcaggc gattgccgcc ccaataggta tggccgcggg tcggcgcacc cctgcgcagg
      781 ccgtgacgga gcgcgggctg aacatgctgt cgctgctggg cgtgctgtcg gtctactggt
      841 gctacgtcgc actgtcgaat gtgctgtacg cgcacagcat gcaggcgacc atcgaggatc
      901 actgggcgtt cgcgccgccg ctgccgcggt tgctgcagca cgcctttctc tacccgttgc
      961 tgatggggag cgtctggata tcactgcgcg tggggtggcg gccggtatgg cgcgccgtgc
     1021 cggtgcaggt gctgatgggc ctgggtttcg cggtgctgtc gcgtccggcg ctgatcgtcg
     1081 gggagatcat cgacggctcg atgggccaca cctgggccaa ggtatttgcc gagcgtgcct
     1141 ctttgatcag tggcaccggg ctcgcgacct gggcggccag caccttgagc tttctgctca
     1201 cctatggttt cggactggcc ctggtcaccg gcctgtcgtg gtatcagcgc tatcgcgatt
     1261 cgcaggagcg cttcgatgcg ctggagcgct cactgtcggc cacacggctg gcggcgctgc
     1321 gcatgcagct ctcaccgcac acgttgttca acctgctgca cacgattcgc ggtcagatcg
     1381 cgtgggatcc gtcctcggcg cagaccatgg tggtgcagct cggcgatgtg ctccggcggc
     1441 tgctggcggc cggcgagcgg gatttttcgc ggctggccga tgaactcgaa ttcgtgcggc
     1501 tgtatctgga attgcagcgc agccgctttt ccgatcgcct gagcttccat atcagcgagc
     1561 tcggtgagct caacaggctc tgggtaccga gcctcattct gcagccactg gtggaaaacg
     1621 cggtcgcgca cggcctgtca ggacatgccg gtgccgtgca cgtgagcgtg gaggccagct
     1681 cggagaacgg gacgctgaag ctcgccgtga ccaacaccac cgcgtccgat accgtcgccg
     1741 gtgcgcaggg aatcgggctg cgcaacgtgc gcgaacgact ggtgctgcac tttccggact
     1801 cggccacgtt tcgtgccagc ccggcggacg ctcagacctg ggtggccgag atccagatgc
     1861 cgctggtgct cgatgcacgc ggcaaccgca gcgcgccttg aacgccgcgg ccgtcatgga
     1921 tgtggtgatc gtggacgacg agccgagcgc gcggcgcacg ctgcgcgaat gctgcgcgcg
     1981 tgagccggat ctgcgggtcg tcggcgaatt cggtgagagc gccggcgcac tcgaagccat
     2041 ccgcgcgcag cggccgcaat tgctgttcct cgacatccag atcgatgcgc tgagtggcat
     2101 ccagatcgcg cgctcactgg acccgtcgca cctgccgctg atcgtattcg tgacggccta
     2161 cgacagttac gcgctcgagg cattcgaggt cagcgcggtg gactacctgc tgaaaccgtt
     2221 cgacgaagag cgcttccgca agacgctggc gcgcgtcagg cgacggcgcg aagccgagtc
     2281 cgtggccgat cgcaatcagg ccctgtcgca ggtgctggcg cagatcgagc gcagcgcccg
     2341 cgccagtgct gacagccggc cgcgcatcct ggcggagtcc ggcagccgca tgcacatgct
     2401 cgacatcgcg caggtcgagg tggcggaggc cgaccgcaac tacgtgaagc tgatcgtcgg
     2461 ccgcgaggtg tatctgacgc gcagtaccct gcagcaggcc gagaaggcgt tgcaatcgca
     2521 atccacactg cgcatcagcc gctcgtgcat cgtgaacgtg acgcatgtac gtgaggtcag
     2581 ccgcaccccc cgcggcgatt tcatcctggt gctcgccggc ggaaccacgg tgaccagcag
     2641 cgaaggctat cgcgatgtgg tgcgcgacta tctcgcgcag ctcaagctcg gggcctgagc
     2701 gcccggcgcc tgcggcactt cgttgccgct caaccacgct tgcagcatcg cattcaccgt
     2761 ttctggagcc tcctgttgta cccagtgcga gacgttcggc aggtagcgca gcgtgagctg
     2821 cggcgcgtag tgatcggtgc cgtaggtgag ctccttgccc agggccgtat ccacttcacc
     2881 ccagagcagc agcgtcggaa cgttgatcgt cgtgatgcgc cgcgcgccga ggcggcgccg
     2941 gccgcgcacc agggcgcggt aatagttgat catcgcggtc agcgcgcccg gctgtgccgc
     3001 gttgtcgcga tagacgcgca gcacctcgtc ggggaaccgg gacttatcca ccgccatgcc
     3061 gcgcaacgcg tgtccgatcg cggcgcagtg atcgcgcgac aggatccatt cggggattgc
     3121 gggaagctgg aagaagagga tgtaccagga ttttcgcatc tgctgcaaag tgcgcagtcc
     3181 cttcgcaaac agcgcggggt gcggcacatt catgatgatc agccgggaga tcggcaggcg
     3241 accgaacatc gcgtagttcc acgcgatcgc cgcaccccag tcatgtccga tcagcagcac
     3301 ctcccggcaa ccggatgctt cgatcaggcc cgcgacatcc tcttccagcc gctcgatcgc
     3361 gtaggcgtcg acgccgagcg ggcgggcagt gccgccatag ccgcgcaggt caggcgccca
     3421 cacctggaag cccaggcttg cgagcagggg tatctgatga cgccacgaaa aggagacttc
     3481 aggaaatccg tgcagacaca gcgccagccg agagccaagc ccttcgaccg cgacatggaa
     3541 atcgatgccg ttggcgtgga tggtgcggta gctgagggtg gccacgggag cgcagtctaa
     3601 ttgaagtacc ttccgctggt ctggaacggg ttgtggcgcc gacgcgccca gactctcttt
     3661 accttggcgt cgatcgccgt cgcattcatc ctgttcggaa tcctggcggg catcgatgcg
     3721 ggttttgcgc atacgctggc tgcctcgcgc atggaccgcc tgttcacgga tccgcgcttc
     3781 ggggcgccga tgccaatgtc ctatggcgag cagatcgccc gcgttcccgg agtcagcgtc
     3841 gtggcgccgc gatcgttgct gttcgggtac ttccaggatc ccaagaacac gctgggcgtg
     3901 ctctgcagcg atcgacgctt ctttgcgctg cgccccgaga tcagcatctc agccccgcag
     3961 atcgagctgt tgcgacagat gcgcaccgga gcggccgtca gtgtcgagct ggccagcaag
     4021 ttcggatgga aggtcggtga caaggtaccg cttcagacca acaccgccca ggccgatggc
     4081 agccgggtct ggaccttcga catcgtggcg atcgtggatg atctcaacta tcccggccag
     4141 ggcggatggt tcatcgccaa ctaccagtac ctggacgagg gccgcgtcga tggcaagagc
     4201 acgatcgacc gcttcctggt gcgcatcagc gatccccggc gcggcgccca gattggccgt
     4261 gagatcgacc agcgcttcgc gaactcggcc gcaccgacgc gcaccaattc cgagcggtct
     4321 caggcgcaat ccggtctgca gttcatcggg gacgtgaatt ttctgaccga cgcggttctg
     4381 ggcgccgtgt tcttcatgct gctgttcctg agcggcaaca ccctgctgca gtcggtgcgc
     4441 gagcgaaccg cggagtttgc gatgctcaag gcactgggtt tctcgaacgt cgccgtgctg
     4501 tcgttggtga tcgccgaatc ggtgctgctg tgtctgctag cggccacggc cggattggtc
     4561 atgtcggaac tgctgatccc gatcgcgcac gacgcgctgg cgaatttcgt cgtcttgctg
     4621 cagatgccgt ggagagcgat gttgcgcgga ttcgctctgg cgctgatcgt cgcgctgctc
     4681 ggcagcctgc tgccggcctg gcgagtcatg cagctcaacg tggtcaacgc gctggtgagg
     4741 cggtagatgg gtgagccgcc gtgttagggg aattcaaaca gattgtgagt ctcacgggga
     4801 ttggcttgag ggccatacct cagcgtaagg gtgcgtccct ggtgatcgtc attggcatgg
     4861 cgctggtcgt cgccgtgacg ctctcgatcc tgtcgatgtc aacgggcttc atgcgcagcg
     4921 ttcgcaacac cggccgcgag gatcgagcca ttgtgctgag ccagggctcg cagttcgagt
     4981 tcgcgagcgc gctgtcgcgc gacaacgtga ccacaatctg cgatgcgccc ggaatcagga
     5041 cggccagcga tggcaagcct atcgtctctg cggagtccct ggccacggtg gcggtcgtca
     5101 agaagagcga tggcctcgat gcctttgtcg cgctgcgcgg catcggccct cagggcttgg
     5161 cgctgcgccc ggagatcaag ctcgtcgcag gtcgcctgtt ccggcccgcg aagcacgagc
     5221 tgattgtcgg gaaatcggcg cgggctcagt tcgccggact tgatctcggg agtcaggtgc
     5281 ccctgccgca gggcgattgg acggtcaccg gcattttcga gagcaatggc gatgggcatg
     5341 agtcggaact gctggctgat tctgagacgg tgtttgccgc catgcgcgcc agcacgttcc
     5401 acagtgtgac cgtgatgctc gagcgagcgc agtccttcga gcagtttcgc acggcgctga
     5461 cgaccaatcc gactctgacg gtcgatgtcg tgcgcgagcc gcagtatctg gccgcccagt
     5521 ccgaatttct gaacgacttc ctgagcatga ttgcctatgt ggtgggcggg atcatgggtc
     5581 tgggcgccgc gtttggcgcg ctcaatacga tgtattcggt cgtcagtgcc cgcgcgatcg
     5641 agatcgccac gctgcgcgcg atcggcttcg gcggcgcagc cgtggtcgcc tccgtgctgg
     5701 tggagtcgct gctgctggcg ctcgccggag cggcgatcgg tgccagcgcc gcctggatag
     5761 cgttcaacgg ggatctgcac gtcgcaggag gcctcgtgtt caaacttgcg gtcacaccgg
     5821 gactcgttgg gctcgggatc ggatttgccg gcacgctggg actgatcggc ggcctgtttc
     5881 ccgcgatccg cgcggcaagg ctgccggtcg tgaccgcatt gaacaaccag tgagattggc
     5941 cggagcccga gccgccccgc tctcagcctc cgggctgctc catcgtgatg actactttgc
     6001 ctcgagcgtg tcccttttcc agatagccga tcgcggccgg gagctcactc aagcggtagc
     6061 gactgtcgat caccggtgtc acctttgcgt cctgcatcaa ctcgcgcaga acgttcaggt
     6121 ccgccttgtt cagattcgcc tggaacgaga cgaccttctg gctcacgaac ggtgacaaca
     6181 ccattgtcct ggctataccg cttatggagt cgaatccgac gatgacgatg atccccgccg
     6241 gtgccaacac gcgccggtat tccaacaccg agcggttgcc cacattatcc agaatcagat
     6301 cgtagcgctg cgcgccccgg gtgaaatcct cccgggtgta gtcgatgacg tgatctgcgc
     6361 cgatcgatcg gaccatctcc acattgcggg tactgcacac accggtcact tctgcaccga
     6421 gggccttcgc aatctgcacg gcgaaggtac ccacgccgcc cgaggcgccg ttgatcaaca
     6481 ccttttgccc cggttggatc cgacccctat cgcgcagtgc ctgcaaagcg gtaatggctg
     6541 cgatcggtgc cgcagcggct tgttcaaacg tcagattctt gggtttcatc accaacgccc
     6601 cgtcggctgg catgcgcaca tactcggcga aggctccgtc ggtgccgccg aacactgaat
     6661 cacccggctg aaactgcttg acgtgggtgc cgaccgcttc caccgttccc gcaaaatcaa
     6721 tgcctgcgcc tgagtacctg ggtctaccga gacccgtctg caggcgcacg acggccgggg
     6781 tgcccctcat gagatgccag tcgatcggat tgacggaggc tgcgtgaatt ctgaccagta
     6841 cttcattgtc tgcgggcgtg ggcttttcga tgtcctccag tttgaggaca tcgggcgaac
     6901 cgtagcatcg ttgggtaatg gccttcatca gagtcttgct ggcggaagca acatccgctg
     6961 gtcggcaagg ggagttgtag ttgagagcca gccccagcga aagcagcatc actgccacga
     7021 ttgccacaac aatccctaaa accctgcggc gaatagtcat caggctatct ggacccgacc
     7081 tgaagtcctg ctcttaacga gccgcagcca gcatagtcca gacagtgacg gtttcccatc
     7141 ccggtcggct acctgaggtc taggcgtcgc gcatcgcctc gacgactggc aggcgcgccg
     7201 cgcgcacggc aggaagtatc gcaccgaaca ggccgatgcc gcatgcccag cccattccca
     7261 atgcgatcag acttaaatca acgtgcaact tggtgagtac ccggcgcgat tcaaatccaa
     7321 gatgggcgcc gatcgtactg ccattgaata gcagccccac gaccagtgca cccaaggcgg
     7381 cgccaatcac ggcaaggaga agtgcctcaa tcaataggga ggccacgaca gcgctgggac
     7441 cgaagccgat ggctcgcagg gtcgcgatct cggtgctgcg ggcactgacg gcggaataca
     7501 tggtattggc cgcaccaaac atggccccca gggccatgat ggcgctcact gtataggcca
     7561 tgaaagtcat ggtgtctcgc acgtgctccg actgctgttt gaagtattcc gactcacggt
     7621 agacgtccag tgaacggaag ggttattcgt gagggcgtct ttgcaggctt cgaacctggc
     7681 aggactttcc agcagcatcg agacggcgtt gaaagcgtta cgacgagtcg cggataagag
     7741 cgtttctgca tcccgccagc agttccgatt cgtgcgtgtc accgtttgac tcgaagattc
     7801 cgacaacagt ccaaggcacc ccgtggattg acggctgctc gcccagacgc agccccttga
     7861 attgcgacga tgcgcctcgg cctaccaaca gttcgtgcac accgggctgc atgacccgcc
     7921 cggcaataat cttgatctcc ggccgcagta agaatgcgcg cggcgcaatt cccctcaccg
     7981 aaacttcggc acctgcatcc tttgagtact cccttactct caaggggact aacacttccg
     8041 ccgatacgat cggctcgcca tcgggcccct gatgcacgcc aggtgcgcct tgg
//
LOCUS       DATMRM010000228         4389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1984611, whole genome shotgun sequence.
ACCESSION   DATMRM010000228 DATMRM010000000
VERSION     DATMRM010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984611"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="VNZ06_06720"
     CDS             <1..1245
                     /locus_tag="VNZ06_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWW20480.1"
                     /translation="PVCVADVGPPPGTTAIDPNLRNDSTAFGEDIHRGYGQTAFFGSA
                     DFDIIPKTLTVTLGTRWYHYTDYELGSKYTTGTECENIANPCYGGSTSIDAENEHKGY
                     HGFKSRGNLTWHITPDILAYYTYSEGFRPGGFNRTIANKAKDAEGNPQYSSPLSYAPD
                     SLINNELGLKSDFFDHRVIANVSLYRMEWHDVQFALFDPTILGNTTFVVNGPNYQIDG
                     AELQLAVKVIDGLTIQGTGSWNSPSQSSSPCLTSNDPKATKAGDPDAAALGQCITEVK
                     GKPFPNPFGVIGTRPAFSPAVEFNLRGRYEFVFGNDFKAFVQAGLSHIGDMQNQPASY
                     LPGDIPSETIPTTTHLRYDQPGYTTYDAAIGIGKDNWSAKLVGENLGNSDASTFTSSA
                     QFIKSEVPLRPRTINLTLGYKF"
     gene            1305..3335
                     /locus_tag="VNZ06_06725"
     CDS             1305..3335
                     /locus_tag="VNZ06_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWW20481.1"
                     /translation="MNSTVTVPPAAASQPSPTELEVNRIRELTKAHHYAEALAAAEAL
                     GAQVPQNRDVLYLTALSQRQQNRIPEALQTLERLEKLHPGFSRLFQERGHCYVVLRDA
                     PKAIEGFLRAVNINPALPASWSMLEGLYRLTGDARNAATAAQHVATLKRLPQEVILAT
                     SLFSDGELTAAEHIIRPFLLEHGNQVEAMRLLAKIGIARDVLDHAQLLLEAVLAIAPD
                     YRAARLDYVVVLLERHKYQQARAELEQLIKAEPDNRQHRAMYATACAGLGEHERAVQL
                     YRKLLREAPQAEEVHLSLAHSLKALGRRDEAIASYHAAATARPDFGDAYWSLANLKTY
                     RFPDEEITRMRAIEAAPEIRLLDRYHVCFALGKAFEDRGDYAESWRFYERGNASKRSE
                     SRYRPEIVEGNTARQISTCTAEFIASRAGVGCGSPDPIFIVGLPRSGSTLLEQILASH
                     SAVEGTQELSDIQRIVLELQGQDPDLANLRYPGVLAELPPEQFLRLGEQFIHDTRVYR
                     AAPKARFIDKNPNNFRHIGLIHLILPNARIIDARREPMGCCFSNLKQLFAKGQEFTYS
                     VEDIARYYRTYLDLMTHWERVLPGRVLRVWHEDVVEDLEGNVRRMLDFCGLPFEPACL
                     AFYRNERSVRTPSAEQVRQPIFRDGLDQWKHYEPWLGPLRESLGDALTRYRQ"
     gene            complement(3354..4142)
                     /gene="phnX"
                     /locus_tag="VNZ06_06730"
     CDS             complement(3354..4142)
                     /gene="phnX"
                     /locus_tag="VNZ06_06730"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016827 - hydrolase activity, acting on
                     acid carbon-phosphorus bonds [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0019635 - 2-aminoethylphosphonate catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HWW20482.1"
                     /translation="MNLKARFDLVIFDWAGTMVDFGCEAPVKALMEAFRAEGVSIDEA
                     AARRDMGKAKIDHVRSLLGYPRVAAEWSKVHGHIADSSDVDVLMARLGPLMRDHAASA
                     STLIPGARQTFERLRAAGLRVASSTGYTREMMQPVLKRAAEQGYKPEHVVCSGETPAG
                     RPSPLMIYKACAELGVWPLSRVVKVDDAEAGIAEGKAAGAFTVGVTSGNQVGLSYDAF
                     HALPSAERNSRVQAARASLLAAGADIVIESVADLIPALSRERAA"
     gene            complement(4139..>4389)
                     /locus_tag="VNZ06_06735"
     CDS             complement(4139..>4389)
                     /locus_tag="VNZ06_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20483.1"
                     /translation="FSLQAVDELILEEFRLSLGVDPPPVLERWTGTYAAASDRPMLLD
                     APHPCVRVAIVTCGAGASTGFAIGEELIASLLDAGVTV"
BASE COUNT          862 a         1482 c         1294 g          751 t
ORIGIN      
        1 cccgtgtgcg tcgccgatgt ggggccgccg cccggcacca ccgcaatcga tccgaacctg
       61 cgtaacgaca gcaccgcgtt cggtgaggac atacaccgcg gttacggaca gaccgcattc
      121 tttggttcgg ccgacttcga catcatcccg aagaccctga ccgtcacgct cggcacacgc
      181 tggtatcact acacggacta cgagctcggc tccaagtaca ccaccggcac cgagtgcgag
      241 aacatcgcga acccgtgcta cggcggatcg accagcatcg atgccgagaa cgagcacaag
      301 ggctatcacg gcttcaagag ccgtggcaac ctgacttggc acatcacgcc ggacatcctc
      361 gcgtactaca cctactcgga ggggttccgg ccgggtggct tcaaccgcac gatcgccaac
      421 aaggccaaag acgccgaagg caatccgcag tacagctcac ccttgagcta cgcgcccgac
      481 tcgctgatca acaacgaact cggccttaag agcgacttct tcgaccatcg cgtgatcgcc
      541 aatgtgtccc tataccgcat ggaatggcac gatgtgcaat tcgcgctgtt cgatccgacg
      601 attctgggca acaccacttt cgtcgtgaac gggccgaact accagatcga cggtgccgag
      661 ctacagcttg ccgtgaaggt catcgacgga ctgacgatcc agggcactgg ctcctggaac
      721 agcccgagcc agtccagctc accgtgtctg acttccaacg atccgaaggc gaccaaggcc
      781 ggcgatccag acgcggccgc gctcggacag tgcatcaccg aagtcaaagg taagccgttc
      841 ccgaacccgt tcggtgtgat cgggacgcgt ccggcattct ccccggcggt ggaattcaac
      901 ctccgcgggc gctacgagtt cgtcttcgga aatgatttca aggccttcgt gcaggcgggc
      961 ctgagtcaca tcggcgatat gcagaaccag ccggccagct acctgcccgg cgacatcccg
     1021 tccgagacca ttcccaccac gacgcacctg cgctacgacc agccgggcta cacgacctac
     1081 gatgctgcca tcggcattgg caaggacaat tggtccgcca aactggtggg tgaaaacctc
     1141 ggcaactcgg atgccagcac gttcacatcc tccgctcagt tcatcaaatc agaggtgccc
     1201 ctgcgtccgc ggaccatcaa cctgacgctg ggctacaagt tctaagcctc ggggattcac
     1261 gtagaatcag ggcgggctgc gatgcccgcc ctttttttgc ccgaatgaat tctactgtca
     1321 ccgtcccgcc cgccgccgct tcgcagccgt cgcccacaga gctcgaagtc aatcgcatcc
     1381 gcgagctcac caaggcgcat cactatgccg aagcacttgc ggccgccgag gcgctgggcg
     1441 ctcaggtccc gcagaaccgc gacgtgctgt acctgacggc cctgtcacag cgtcagcaga
     1501 acaggattcc cgaagcgctg caaacgctcg agcggctcga gaagcttcat ccgggattca
     1561 gtcggctgtt ccaggaacgc gggcattgct acgtggtact gcgtgatgcg cccaaggcga
     1621 tcgaaggatt cctgcgagcg gtcaacatca atccggcgct gccggcaagc tggagcatgc
     1681 tggagggact gtatcggctc accggcgacg cccgtaacgc cgccaccgcc gcgcagcacg
     1741 tggctacgct caagcgattg ccgcaggaag tgatcctcgc cacgagcctg ttctccgatg
     1801 gtgaactgac cgcggccgag cacatcatcc gaccgtttct gctcgagcac ggcaaccagg
     1861 tcgaggccat gcggctgctg gccaagatcg gcattgcgcg cgatgtattg gaccatgcgc
     1921 agctgctgct cgaggcggtg ctggcgatcg ctccggacta tcgcgccgca cgcctggact
     1981 atgtcgtggt gctgctggag cggcacaaat accagcaggc gcgcgcagaa ctggagcaac
     2041 tcatcaaggc cgagcccgac aaccgccagc atcgcgcgat gtacgcgacc gcctgtgcag
     2101 gtctgggcga acatgaacgc gcggtgcagc tctacagaaa gctgttgcgc gaggcaccgc
     2161 aggccgagga agtgcatctg tcgctcgcgc attcgctaaa ggctttgggc aggcgtgacg
     2221 aggcgattgc ctcctatcat gcggcggcga cggcacgccc ggacttcggg gatgcctact
     2281 ggagcctcgc gaacctcaaa acctatcgat ttccggacga ggaaatcaca cgcatgcgcg
     2341 cgatcgaggc cgcccccgag atccggctgc tcgatcgtta ccatgtgtgc ttcgcgctcg
     2401 gcaaggcctt cgaggatcgt ggagactacg ccgagtcatg gcgcttctac gagcgcggca
     2461 atgcatcgaa acgctccgag agccgctatc ggccggagat cgtcgaaggc aacaccgcgc
     2521 ggcagatcag cacctgcacc gccgagttca tcgccagccg ggccggcgtc ggctgcggca
     2581 gtccggatcc gattttcatc gtcggcctgc cgcgctcggg ctcgacactg ctcgagcaga
     2641 tcctcgcctc acactccgca gtcgagggca cccaggaact gtccgacatc cagcgtatcg
     2701 tgctggaatt gcaggggcag gatccggatc tcgccaacct gcgctacccg ggcgtgctgg
     2761 cggaattgcc cccagagcag ttcctgcgcc tcggcgagca attcatccat gacacacgag
     2821 tctatcgcgc tgctcccaag gcgcgtttca tcgacaagaa tcccaacaac tttcgtcaca
     2881 tcgggctgat ccacctgatc ctgccgaacg ccaggatcat cgatgcgcgg cgcgaaccga
     2941 tgggctgctg cttcagcaat ctcaaacagc ttttcgccaa aggtcaggaa ttcacgtaca
     3001 gcgtcgagga catcgcgcgc tactaccgca cctatctgga tctgatgacg cactgggaac
     3061 gggtgctgcc cggacgcgtg cttcgggtct ggcacgagga cgtggtggag gatctcgagg
     3121 gtaacgtcag gcgcatgctg gatttctgcg gactgccgtt cgagccggcc tgtctggcgt
     3181 tttaccgcaa cgaacgcagc gtgcgcacac cgagcgcgga gcaggtgcgc cagccgatct
     3241 tccgcgacgg gctcgatcag tggaagcact atgagccgtg gctggggccg ctgcgtgagt
     3301 cactcggtga tgcactcacc cgctatcgtc agtaagctag cggctgcgcg atcttaggcc
     3361 gcccgctcgc ggctcagcgc cggaatcaga tcggccacgg actcgataac aatgtcagcc
     3421 ccggccgcca gcagcgacgc tcgtgcggcc tgaacgcggc tgttgcgctc cgcgctcggc
     3481 agcgcatgaa acgcatcgta ggacaggccg acctgattac ccgaggtcac acccaccgtg
     3541 aatgcgccgg cggccttgcc ctcggcgatg ccggcttctg cgtcatcgac cttgaccacc
     3601 cgtgacagcg gccacacgcc cagctccgcg cacgccttgt aaatcatcaa cggcgacggc
     3661 ctgccggcgg gcgtctcccc ggaacacacg acgtgctcgg gcttgtaacc ctgttccgcg
     3721 gcgcgcttca ataccggctg catcatctcg cgggtgtagc cggtcgatga ggcaacgcgt
     3781 aggcccgcag cccgcagccg ctcgaaggtc tgccgcgcac cggggatgag cgttgaggca
     3841 ctcgccgcat gatcgcgcat cagaggcccg agccgtgcca tcagcacgtc cacatcgctc
     3901 gagtccgcga tgtgtccgtg caccttgctc cattcagccg cgacccgcgg atagccgagc
     3961 aggctgcgca cgtgatcgat cttggccttt cccatgtccc gccgggcggc ggcctcatcg
     4021 atcgatacgc cttcggcgcg gaaagcctcc atcagcgcct tgaccggcgc ttcgcatccg
     4081 aagtccacca tcgtgccggc ccaatcgaag atcaccaggt cgaagcgcgc tttgagattc
     4141 ataccgtcac tccagcatcc agcagacttg ctatcaattc ttcgccgatc gcaaaaccgg
     4201 tggaggcacc tgcgccacag gtcacgatcg ccactcggac gcaagggtgc ggcgcatcca
     4261 acagcattgg ccgatcgctc gccgcggcgt aggttccggt ccagcgctcc agcaccgggg
     4321 gtggatccac ccccaggctc aagcggaatt cttccagaat taattcatcc acggcctgca
     4381 gtgaaaagg
//
LOCUS       DATMRM010000229         7595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_120720, whole genome shotgun sequence.
ACCESSION   DATMRM010000229 DATMRM010000000
VERSION     DATMRM010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120720"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="VNZ06_06740"
     CDS             complement(<1..1305)
                     /locus_tag="VNZ06_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWW20484.1"
                     /translation="MTARLMAISLLVLLSSCHRSQPLHAQHALHEPKDISWFDGSMEG
                     AFTVAKRENRPVLLFWGAEWCPYCQTLKSTVFPRPDFIAKSHLFLPVYLDGDDDGAQK
                     WGEQFGVQGYPTLIVLDADRHEIIRLGAGRDVSQYAAVLDLALDNIQPADALLQTAAG
                     GRVLTDNECRRLAYNSWDLDTLDAGSYAARAQQLQAAAAQCPEDLKLERAQLAIYAAF
                     YAANAESDAPDTPKGQPSAKLVASTDQVGAILKQQELAVSAAGALQVLDDAFFKAVHA
                     RGPKVAPQMRDSFIAVMDAASVDTRYAVADQLGFIDAKLRALKALNDTKYKYPKELTA
                     AFDQRIDDALAAEQNQWLRSGLVNASLNILEDTGDYNKAYDIAKAEMARSATAYYYEG
                     DLAEIAEKLGRQDEAISLLDQAYRDSQGPATRFQWGQRYVSGL"
     gene            complement(1386..3947)
                     /gene="hrpB"
                     /locus_tag="VNZ06_06745"
     CDS             complement(1386..3947)
                     /gene="hrpB"
                     /locus_tag="VNZ06_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HWW20485.1"
                     /translation="MPSDLTEALSAQQRALTRSLGAVFDQHRDAVLHAPTGAGKSTLV
                     PLALLGELSDPGQKILMLEPRRLAARAVAARMAALLGEPIGRTVGYRMRQDTRVSSLT
                     RIEVLTEGVLTRLLQEDPGLEQVAYLIFDEFHERSLAADLGLALALDARAQLDAAFRI
                     LIMSATLECERVAAVLGDAGVASVPGRAFPVALQYLGQGAPLFPSGAQPLERMVVPAV
                     QRALADGYADVLVFLPGAGEIRRMETALAQSIPSSGRLILPLYGELSADSQDAVLAPT
                     PPGRRKVVLATNIAETSLTIPGVTAVVDCGLARRSRFDPSTGMSRLELVRISQAASEQ
                     RAGRAGRVAPGVCYRLWGESAQARLAASTPAQILEADLASLALELGGWGITDVQRLRW
                     LDAPPAAALQQARELLQRLDALDEHAMPTALGREMMRLPVHPRLAHMLLASRELGAVE
                     HAAQLAALLSERDLLRRDSGTAVRDPDIRSRLEVLRGVRTTSAVDRPTLQRVLRTAHS
                     LADMLGSRRPTERSAQAQDLTGALLSLAYPDRIGQRRSEGEGRYLLNSGRGAMFRDAT
                     SLAREEFIVASSLDDAEREARVDLAAPIARSQIERLFAARIVQSDEFGWDSRESAVLA
                     RRTRRLDALRLDTQLLNASGDARAVDAMLEGIAQLGLTALPWDNESRQLQARMEFVRG
                     LARADLADWPVSDDAALMATRTDWLGGHLPGITRREHLSRLDLTRALRARLTSAQSRA
                     LDQLAPRALTVPSGSQIRIDYVDDNAPSVAVKLQEVFGLQQTPRVGDGAVAVTFKLLS
                     PAQHPVQITRDLAGFWRTSYAEVRKDMRGRYPKHIWPENPLEAAPTRRTVRSPKR"
     gene            complement(3937..6516)
                     /gene="mprF"
                     /locus_tag="VNZ06_06750"
     CDS             complement(3937..6516)
                     /gene="mprF"
                     /locus_tag="VNZ06_06750"
                     /inference="COORDINATES: protein motif:HMM:NF033480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HWW20486.1"
                     /translation="MDTAHAEQEVKEIWRRWLLPGVSLLIFIGVAFALHRQLSQFRLS
                     TVLNHLRSIPKTTVLAAIFWTAISYWLLGFYDVLALRYLGKKVAYGRALFTSFIAYSF
                     GHNFGIASLTGGAVRYRLYSTAGLSAADVATVAGFCMVTSAIGLGALAAISFVYVPHQ
                     ASHLLHINHNIVLAGGVLLFAGIAIYALWSLAGPRQIELFNYALRAPRPLVALPQIVL
                     AVVDLGVSAIVLYLLLPAGSDVTFVAFLGAYSIAITAGIASHVPGGIGVFESLIVLGL
                     PSVPADQLIGSLLAWRAVYYLLPLLVAALLFASQELRSQASTLARIEQRAAAYVAPIV
                     PQITGSLVVAAGFLLLVSGATPSIDARLSILRDVLPLVVLEFSHLAGSVIGLALLILA
                     RALFLRVRAAYQLTVYLLIAGMLTSLLRGLEIEQSLLLGVVLVILWLGRRAFYRPSEI
                     LSQPFTPSWIVSLAIVISTAVWIGFFAYRHVDYSHSLWWTFAFEADAPRMLRASLVVV
                     LLAAAYLAMNLMQPARPEPGVASTEDLSIARPLISGSSSAIANAAISGDKRILFSSKR
                     DAFLMYQVNGRSWVALGDPVGTAEGQEELVWQFRELSDRHGGWSVFYQVSADCLPLYV
                     DLGLAVMKLGEEARVHLPDFSLEGSARAELRTQRRRAEKDGATFEVVTPDALEPLLPQ
                     LRAVSDAWLEDKAVAEKSFSVGSFSESYVRNFPVALVRVEGEVVAFATLWIAGTGEEI
                     AVDLMRFGPDAPRGAMDFLFVELLLWGRAQGYRWLSLGMAPLAGLEKHPLAPAWHRVG
                     NIVFRFGEYFYNFDGLRRYKAKFNPVWESKYLAAPGGLALPRVLLDVSMLISGGVKEL
                     FAK"
     gene            complement(6521..6916)
                     /locus_tag="VNZ06_06755"
     CDS             complement(6521..6916)
                     /locus_tag="VNZ06_06755"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HWW20487.1"
                     /translation="MQVQPRRFCQLAAILLGLATVIGALGSHALRSRLPAARYEVLQT
                     AVHYQYFHSLGLLGLGLLLDRVGAPLLRLAGWLLFAGVLLFSGSLYLILAGAPRFIGA
                     LTPVGGLALILAWGLAAWALGAPSSDRAS"
     gene            complement(6930..>7595)
                     /locus_tag="VNZ06_06760"
     CDS             complement(6930..>7595)
                     /locus_tag="VNZ06_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HWW20488.1"
                     /translation="VRYDLAIESPEYVQELAQHHVGGYLKIAPEHLREGPLSKMMKPG
                     VGAYYRFKELFDRYSKAAGKEQYLIPYFIAAHPGTTDEDMLELALWLKRNGFRADQVQ
                     AFLPSPMASATAMYHSGKNPLKRITRDSEQVFIPKGLRVRRLHKAFLRYHDPNNWPLL
                     REALRRMGRAELIGNAREQLIPAYQPPGSGTRPKGARAFRTQHTGLPRTPAAPRKAPR
                     GRH"
BASE COUNT         1441 a         2608 c         2354 g         1192 t
ORIGIN      
        1 caggccgctg acatagcgct ggccccactg gaagcgcgtg gccggtccct gcgaatcgcg
       61 gtacgcctga tcgagcaggc tgatcgcttc gtcctgacgt cccagcttct cggcgatctc
      121 ggccagatcg ccttcatagt agtaagccgt tgccgagcgc gccatctcgg ccttcgcaat
      181 atcgtaggcc ttgttgtagt cgccggtatc ctcaaggatg ttcagcgagg cattcaccag
      241 gccggatcgc agccactggt tctgctcggc ggccagtgca tcgtcgatgc gctgatcgaa
      301 cgcggcggtc agctccttcg gatatttgta cttcgtgtcg ttgagcgcct tgagtgcgcg
      361 cagtttggcg tcgatgaaac cgagctgatc ggccaccgcg taacgcgtgt ccacggaggc
      421 tgcgtccatg accgcgatga aactgtcccg catctgcggc gcgactttgg ggccgcgcgc
      481 gtgcaccgct ttgaagaagg cgtcatccaa cacctgcagc gcacccgccg cggagacggc
      541 cagttcctgc tgcttcagga tggcacccac ctggtccgtg gacgcgacca gcttggccga
      601 gggctgcccc ttgggggtat ccggagcgtc gctctcagcg ttggcggcat agaaggcggc
      661 atagatcgca agctgcgcgc gctccagttt caggtcctct ggacattgcg cggctgcggc
      721 ctgcagctgt tgcgcgcgcg ccgcgtagct gcccgcatcg agcgtatcga gatcccacga
      781 gttataggcc agacgacgac actcattgtc tgtcagcacc cgaccacccg cggccgtctg
      841 cagcagcgcg tccgcaggct gaatgttgtc gagcgccaga tccagcaccg cggcgtactg
      901 cgatacatcg cgcccggcgc cgagccggat gatctcgtgc cgatcggcat ccaatacgat
      961 cagggtcgga tagccctgga cgccgaactg ctcaccccat ttttgcgcac cgtcatcatc
     1021 gccatccaga tagaccggca ggaacaggtg gctcttggcg atgaaatccg gccgcgggaa
     1081 cactgtggat ttcagagtct ggcaatacgg gcaccactca gcgccccaga acaacaggac
     1141 cggccgattc tcgcgcttgg cgacggtgaa agccccttcc atgctgccat cgaaccagga
     1201 gatatctttc ggctcgtgta gcgcgtgctg agcgtgcagg ggctgcgagc ggtggcagct
     1261 cgacagcaga accagcaggc tgattgccat caggcgtgca gtcattctca taggataccg
     1321 caacgcgcga agcgcaccct cgttaaccca tcttcacaaa tatacggcgc ccgctcagcg
     1381 ctcacttacc gcttgggcga gcgcacggtc cgtcgcgtcg gcgcagcttc cagcggattt
     1441 tccggccaga tgtgcttggg gtaacgcccc cgcatgtcct tgcgcacctc ggcataactg
     1501 gtgcgccaga aacccgccag atcgcgggtg atctgcaccg ggtgctgtgc cggtgaaagc
     1561 agcttgaaag tgaccgcgac cgcgccgtca ccgacgcgcg gggtctgctg caggccaaat
     1621 acctcctgca gtttgacggc aacgctcggt gcgttgtcat cgacatagtc gatccggatc
     1681 tgcgagccgc tgggcacggt cagcgcacgc ggtgcgagct ggtccagggc gcgcgactgc
     1741 gcgctcgtca gccgcgcccg cagcgcgcgt gtcagatcca gcctcgacag gtgttcgcga
     1801 cgcgtgatgc ccggcagatg tccgccaagc cagtccgtac gcgtcgccat caaggccgcg
     1861 tcatcgctca ccggccagtc cgccagatcc gctcgggcga gcccgcgcac aaattccatc
     1921 cgggcctgca gctggcggct ctcgttgtcc catggcagcg cggtcaggcc aagctgcgca
     1981 atgccctcca gcatggcatc cacggcgcgc gcatcaccgc tggcgttcaa gagctgagtg
     2041 tcgagccgca gcgcatccag ccggcgcgtt cgccgagcga gcaccgcgct ttcgcgactg
     2101 tcccatccaa actcatcgct ctgcacgatt cgggcagcga acagacgctc gatctgggag
     2161 cgcgcgatgg gtgccgccag atcgactcgt gcctcgcgct cggcatcatc cagagagctc
     2221 gccacgatga actcctcgcg ggccagagag gtcgcgtccc ggaacatcgc accgcgcccc
     2281 gaattcagca aatatcgccc ttccccctca ctgcgacgct gaccgatgcg gtccggatac
     2341 gccagcgaca gtaacgctcc ggtcagatcc tgggcctgag cgctgcgttc tgtcgggcgt
     2401 cgcgacccga gcatatcggc gagtgaatgt gccgtacgca gtactcgctg cagcgtcggg
     2461 cgatccactg cggaggtcgt gcggaccccg cgcagcacct cgagccgact gcggatatcc
     2521 gggtcgcgca cggcggttcc ggaatcgcgc cgcagcaggt cgcgctcaga cagcagcgcg
     2581 gccagttgcg ccgcgtgttc gacagcgccc aattcccgcg aggccagcag catgtgcgcc
     2641 aggcggggat ggaccggcag ccgcatcatt tcccttccca acgcggtcgg catcgcatgc
     2701 tcgtccaggg catccaggcg ctgcagcagc tcacgcgcct gctgcaacgc cgccgcgggc
     2761 ggcgcatcca gccagcgaag tcgctgcaca tcggtgattc cccaaccccc gagctcgagt
     2821 gcgagcgatg caaggtccgc ttcgagtatc tgggccgggg tactggctgc aaggcgcgcc
     2881 tgcgcgcttt ctccccacag ccggtagcac acgcccggcg cgacgcgacc ggcgcggccc
     2941 gcgcgttgtt cactcgctgc ctgcgagatg cgtaccaact cgagccgact catgcccgtg
     3001 gatggatcga agcgcgatcg acgcgccaac ccgcaatcca ccaccgcggt cacgcccggt
     3061 atcgtgagac tggtctctgc aatgttggtc gccagaacca ctttgcgccg ccccggcggt
     3121 gtgggggcca gcaccgcatc ctgactatcg gcggacagtt caccgtagag cggcagaatc
     3181 aatcgtcccg aggaagggat actctgtgcc agcgctgtct ccatccgccg gatttcgcct
     3241 gctcccggca agaacaccag tacatcggcg tacccgtccg ccagcgcgcg ctgcacagcg
     3301 ggtacgacca tgcgctcaag cggctgcgcc ccgcttggaa acaacggcgc cccctgcccc
     3361 agatactgca gcgccaccgg gaaggcgcgt ccgggaacac tcgccacgcc agcgtcgccc
     3421 agcacagctg caacgcgctc acactcgagg gtcgccgaca tgatcaggat gcgaaacgcg
     3481 gcatccagtt gcgcgcgagc atcgagcgcc agcgccaacc ccaagtccgc agcgaggctg
     3541 cgttcgtgga attcgtcgaa gatcaggtag gcgacctgct cgaggcccgg atcctcctgc
     3601 agcagccgcg tcagcacgcc ttcggtcagt acctcgatgc gggtgaggct ggagacgcgg
     3661 gtatcctgtc gcatgcgata gccgacggtc cggccgatgg gttctccgag cagtgcggcc
     3721 atgcgcgccg cgaccgcgcg cgccgccagc cggcgcggct ccagcatcag aatcttttga
     3781 ccgggatccg acagctcacc cagcagcgcc agtggcacga gcgtgctctt gcccgcaccc
     3841 gtgggggcgt gcagcacggc gtcgcggtgc tgatcgaaca cagcgccgag cgagcgcgtc
     3901 agggcacgct gctgggccga cagcgcttcg gtcaaatcac ttggcaaaca gttccttgac
     3961 gccgccggag atcagcatcg atacgtcgag cagcacacgc ggcagcgcaa gaccgccggg
     4021 tgcagccaga tacttcgact cccacaccgg attgaacttg gctttgtagc ggcgcagacc
     4081 atcgaagttg taaaaatact cgccaaaccg gaacacgatg ttgcccacgc gatgccacgc
     4141 cggggcgagc ggatgctttt ccaggcccgc caacggtgcc atccccaagc tcagccagcg
     4201 atacccctgc gcacgtcccc acagcagcag ttcgacgaac agaaagtcca tcgcaccacg
     4261 cggcgcatcc ggaccgaacc gcatcaggtc caccgcgatc tcctctccgg ttccggcgat
     4321 ccacagcgta gcgaacgcga ccacctcgcc ttccacacgc accagtgcga ccggaaaatt
     4381 ccggacgtag ctctccgaga aggatccgac cgagaagctc ttctccgcta ccgccttatc
     4441 ctcgagccag gcgtccgata cggcgcgcag ttgcggcagc agcggctcga gcgcgtcggg
     4501 cgtcacgacc tcaaatgtcg caccgtcctt ctccgctcgc cgccgctggg tgcgcagttc
     4561 ggcgcgcgcg ctgccctcga gcgagaaatc cggcaggtgc acccgtgcct cttcgccaag
     4621 tttcatcacc gcgagcccca gatcgacgta cagcggcaga cagtcggcgc tcacctgata
     4681 gaacaccgac cagccgccgt gccggtccga cagctcgcga aactgccaca ccagttcttc
     4741 ctgaccttcc gccgtgccta ccggatcgcc gagcgccacc cagctgcgac cgttgacctg
     4801 atacatcaga aacgcgtcgc gcttgctgct gaacagaatg cgcttgtcac cgctgatcgc
     4861 ggcattggcg atcgcactcg aactgccgct gatcagcggc cgcgcgatcg acagatcctc
     4921 ggtggaggcc acaccgggct ctggccgcgc gggttgcatc aggttcatcg ccagataggc
     4981 ggccgccagc agcacgacca ccagtgatgc gcgcagcatg cgcggagcat ccgcctcgaa
     5041 agcaaacgtc caccacagcg aatgggaata gtccacgtgc cggtaggcaa agaagccgat
     5101 ccacaccgcg gtggaaatca caatcgcgag gctcacgatc cacgatggcg tgaacggctg
     5161 cgacaggatt tcggacggcc ggtagaacgc gcggcgcccc agccacagga tgaccagcac
     5221 cacacccagc agcagggact gctcgatctc aaggccccgc agcagcgaag tcagcatgcc
     5281 ggcgatcagc agatagactg tcagctgata ggccgcacgc acccgcagga acagcgcgcg
     5341 tgccagtatc agcagcgcca ggccgatcac gctgccggcc aggtgtgaaa actccagcac
     5401 caccagcggc agcacatcgc gcaggatcga caaccgcgca tcgatcgagg gcgtggcgcc
     5461 tgacaccagt agcaggaagc cggccgccac caccagcgag ccggtaatct gcggcacaat
     5521 cggtgccacg taggccgcgg cgcgttgctc gatccgggcg agcgtcgaag cctgagagcg
     5581 cagttcctgg ctcgcgaaca gcagcgctgc caccagcagc ggcagcaggt agtacaccgc
     5641 gcgccaggcg agcagcgagc cgatcaattg atccgctggc acactcggca gacccagcac
     5701 gatcagcgat tcgaagacgc cgatcccccc gggcacgtgg ctggcgatgc ccgccgtgat
     5761 tgcaatggaa taggcgccga gaaatgccac gaacgtgaca tcgctgccgg ctggcaacaa
     5821 cagatacagc acgatcgccg atacccccag atccaccacc gccagcacga tctgtggcaa
     5881 tgcgaccagc ggcctgggtg cgcgcagcgc gtagttgaac agttcaatct gccgcggacc
     5941 cgccagcgac cagagcgcat agatcgcgat gcccgcaaac agcagcacac cgccggcgag
     6001 cacgatgttg tgattgatat gcagcagatg tgaggcctgg tgcggcacgt agacgaatga
     6061 aatggcggcc agcgcaccca atccgatcgc gctggtcacc atgcagaagc cggccacggt
     6121 cgcgacgtcc gcggcactca gccccgcggt cgagtacagc cggtaacgca ccgcgccgcc
     6181 ggtcaggctc gcgatcccga agttgtggcc gaaggagtag gcgatgaacg aggtgaacag
     6241 tgcacggcca taggccactt tcttgccgag atagcgcagc gccagcacat cgtagaagcc
     6301 cagcagccag tagctgattg cggtccagaa gatggctgcc agcacggtcg ttttcggaat
     6361 cgacctcaga tggttcaaca ccgtcgagag ccggaattgc gacagctgac ggtgcagcgc
     6421 gaaggcgaca ccgatgaata tcagcagcga taccccgggc agcagccagc gccgccagat
     6481 ctccttgacc tcctgctctg cgtgcgcggt atccacggct ttaagaggcc cggtccgagc
     6541 tcggcgcgcc caaggcccat gccgccaggc cccaggcgag aatcagcgcc agaccgccga
     6601 ccggcgtgag cgcgccgatg aatcgcggtg ccccggccag gatcagatag aggctgccac
     6661 tgaagagcag cacaccggca aacaatagcc accccgccag gcgcagtagc ggcgcgccga
     6721 cgcggtccaa cagcaggcct aaacccaaca gtcccagcga gtggaagtac tgatagtgaa
     6781 ccgcagtctg caacacctca tagcgcgcgg ccggcagacg gctgcgcaac gcgtgcgacc
     6841 cgagcgcccc gatgaccgtc gcgagcccca gcaggatcgc tgccagctga cagaaccgtc
     6901 ggggctggac ctgcatgaga cgtcaaaccc tagtgccggc cgcgcggggc tttgcgcggt
     6961 gccgccggcg tgcgtggcag cccggtatgt tgagtacgga acgctcgggc gcccttcggc
     7021 cgggtcccgc tacccggtgg ctgatacgcc ggaatgagct gctcgcgggc gttgccgatc
     7081 aactcggcgc gtcccatgcg tcgcagcgct tcgcgcaaga ggggccagtt gttcgggtcg
     7141 tgatagcgca ggaaagcctt gtgcagacgc cgcactctga gcccctttgg tataaacacc
     7201 tgctcgctgt cgcgcgtgat ccgtttcagc ggattcttgc cgctgtggta catcgccgtc
     7261 gcgctggcca tcggtgaggg caggaaggcc tgtacctgat cggcccggaa accattgcgc
     7321 ttgagccaca gcgccagttc gagcatgtcc tcgtcggtcg tacccggatg cgcggcgatg
     7381 aagtaaggaa tcagatactg ctccttaccg gccgccttgg agtaccggtc aaacagctcc
     7441 ttgaagcggt agtaggcgcc gacaccgggt ttcatcatct ttgacagcgg accttcgcgc
     7501 agatgctcgg gcgcgatctt cagatagccg cccacgtggt gctgcgcgag ttcctgaacg
     7561 tactcggggg attcgatcgc gaggtcgtag cgcac
//
LOCUS       DATMRM010000230         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1374311, whole genome shotgun sequence.
ACCESSION   DATMRM010000230 DATMRM010000000
VERSION     DATMRM010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374311"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VNZ06_06765"
     CDS             complement(<1..798)
                     /locus_tag="VNZ06_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWW20489.1"
                     /translation="MNDDSALIEDAARRLFRELGQPESLKSAADRDRLWDALNAAGLT
                     RTWIAEALGGSGASLSDGFAVLRIAGEHAAAIPLAETLLAGWLLQQGQISMPASRLSI
                     APVDVRDQIELSQSDTLTGTARAVPDARECGQFAVLAARDKRSYVALLDATQVRIAHH
                     TNVAGEARDTLTIEQATPIALAPTSSGLDEIMLVGAVARASQMAGALQAILELSVTYA
                     NERVAFEKPIGKFQAIQHSLARLAGEAAAAIAAAGSAADAMDTAACFD"
     gene            complement(795..1811)
                     /locus_tag="VNZ06_06770"
     CDS             complement(795..1811)
                     /locus_tag="VNZ06_06770"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HWW20490.1"
                     /translation="MAAKKKSTHPQVKSRAKKRTRGKLAPGANARGLEASEVAIGLDA
                     AEIAPLVELVRSAGGAAIGAYREPLGARALLLASLPLGAIQPTPFQRDLSPTHAKRLA
                     QKIDETAAFLDPLIVVRGEDGRFWTPNGRHRLAAGKVLGMKQIMALISPEDALAYRIL
                     ALNTEKAHNLKDRSLEVVRMARSLARQQPRAPESQFVAEFESPELLTLGVVYERSPRF
                     AGGAYGAMLKKVDRFSHGALPASLRERDSFAARLTEIDALVKKIMDALQAKGFKSPYL
                     RNYVVARINPVRFHKVKKGDTKPPMPLAQALTRMAVAAKTFKLDSISHSDLAWVAVGA
                     GSAE"
     gene            complement(1811..>2513)
                     /locus_tag="VNZ06_06775"
     CDS             complement(1811..>2513)
                     /locus_tag="VNZ06_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWW20491.1"
                     /translation="KQLFGNDVFVSGCRPGSTNPNCADQKGPDYDFGNSPIIAQVPGG
                     KSIIVIGQKSGNGWGLDPDNQGAVLWQYHAGAGGALGGIEWGSAVDAEHVYFAVSDIF
                     TPSPGGLHAVNLKDGSKAWYAAPAPPTCAKGGRCNAAQAAALSVIPGVVFSGSNDGTL
                     RAYSTTDGSVIWSVNTEKEFPTVNGVSARGASMLGPGPVIAGSMLYVPSGYGAFGGRP
                     GNVLLAYGLAEDHGK"
BASE COUNT          432 a          823 c          788 g          470 t
ORIGIN      
        1 gtcgaaacac gccgcggtat ccatcgcatc cgcagcggat cccgcggcag caatggccgc
       61 tgcggcttcg ccggccaggc gcgcgaggct gtgctggatt gcctggaact taccgatcgg
      121 cttttcgaat gcgacgcgct cattggcgta agtcaccgac aactccagga tcgcctgcaa
      181 cgctccggcc atttgcgacg cgcgggcgac ggcgcccacc agcatgatct catccaatcc
      241 gctgctggtc ggtgccagcg cgatcggcgt ggcttgttcg atcgtgaggg tgtcgcgtgc
      301 ctcgccggca acgttcgtgt gatgggcgat tcgaacctgg gtcgcgtcca gtagtgcgac
      361 gtaggagcgc ttgtcgcgcg cggccagcac ggcaaactgc ccacactcgc gcgcatcggg
      421 caccgcacgc gcagtgcctg tgagcgtgtc ggactggctt agttcgatct ggtcgcgcac
      481 gtccaccggg gcgatcgaca gcctgctcgc aggcatcgaa atctgcccct gctgtagcag
      541 ccagccggca agcagcgttt ccgcaagcgg aatggccgca gcgtgttcgc ccgcgattcg
      601 caggaccgcg aacccgtccg acagggatgc gccagagccg ccgagagcct cggcaatcca
      661 ggtacgcgtg agtcctgcgg cattcagcgc gtcccagagc cgatcccggt cggcagcgct
      721 cttgagcgac tcgggctgtc cgagttcgcg gaacagccgt cgcgccgcgt cctcgatcag
      781 cgccgagtcg tcgttcattc ggcgctaccc gcaccgacgg cgacccatgc gagatcggag
      841 tgagaaatgg agtcgagctt gaacgtcttg gccgctacgg ccatgcgtgt cagtgcctga
      901 gccagaggca tcggtggctt ggtgtcgccc ttcttcacct tgtggaagcg gaccggattg
      961 atgcgtgcca caacgtagtt gcgcagatag ggcgacttga agccttttgc ctgcagcgca
     1021 tccatgattt tcttcaccag agcgtcgatc tcggtcagac gcgccgcaaa gctgtcgcgt
     1081 tcacgcagac ttgcgggtag cgcaccatgg ctgaaacggt cgaccttctt cagcatggcg
     1141 ccatacgcac cgccggcaaa gcgcggagag cgttcgtaaa ccacgcccag cgtcagcagc
     1201 tccggcgatt cgaattccgc gacgaactgt gactcgggcg ctctgggctg ctgtctagcc
     1261 agactacgcg ccatacgaac cacttccagg ctgcgatcct tcaggttgtg cgccttttcg
     1321 gtgttgagtg cgaggatgcg ataggcgagg gcatcctccg gcgagatcag cgccatgatc
     1381 tgcttcatgc cgagcacctt accggccgcc agtcgatgtc gaccgttcgg ggtccagaaa
     1441 cgaccgtctt cgccgcgtac gacgatcagc gggtcgagga acgccgcggt ctcgtcgatc
     1501 ttctgcgcca ggcgcttggc gtgggttggc gacagatcgc gctggaaggg tgtgggctgg
     1561 atcgctccca gcggcaggct cgccagcagc agggcgcgcg ctccgagcgg ctcacgatag
     1621 gccccgatcg ccgctccacc cgcgcttcgg accagctcaa ccagcggtgc gatctccgca
     1681 gcatccaggc cgatagccac ttcggaggct tccaggccgc gggcgttcgc acctggggcc
     1741 aacttgcctc gggtgcgttt cttagccctc gattttacct gcgggtgggt gctctttttc
     1801 ttcgctgcca ttacttcccg tgatcctcgg caagcccata tgccagcagt acgttgcccg
     1861 gacggccgcc aaaagcgccg tatccggacg gtacatatag catggagcct gcgatcacgg
     1921 gaccgggccc gagcatggag gcgcctctgg cggacacccc attgaccgtc gggaattcct
     1981 tctcggtgtt gaccgaccag atcacgctgc catcggtggt cgagtacgcc cgtagcgtgc
     2041 cgtcattcga accggagaac acgacccccg gtatgacgct gagggctgcc gcctgagcgg
     2101 cattgcagcg cccgcccttg gcacaggtgg ggggagccgg tgccgcgtac cacgcctttg
     2161 agccatcttt caggttcacc gcatgcaggc cgcccggaga gggcgtgaag atgtcggata
     2221 ccgcgaaata gacgtgctcg gcatcgaccg ctgagcccca ctcgatacca cccagggcgc
     2281 caccggcccc ggcatggtac tgccacaata ccgccccctg gttgtctgga tcgagtcccc
     2341 agccgttacc cgacttctgg ccgatcacga taatcgattt gccgccaggg acctgcgcga
     2401 tgatcggtga attgccaaaa tcgtagtccg gacccttctg atcggcgcaa ttgggattgg
     2461 tacttccagg cctgcagcca ctgacgaaaa catcgtttcc gaaaagctgt ttg
//
LOCUS       DATMRM010000231         3104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3525164, whole genome shotgun sequence.
ACCESSION   DATMRM010000231 DATMRM010000000
VERSION     DATMRM010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3525164"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VNZ06_06780"
     CDS             <1..372
                     /locus_tag="VNZ06_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20492.1"
                     /translation="ALNSVPILRFRCRTCSRTCSRLPLCVAPRRWYSWALQQQVLLLL
                     LAGCSLRRASSIFALGRHTVQRWRQWLDERSLAFEFFLRSRFAELGRAVDRPAFWLGC
                     LQRMPLCEAMAWLDGDGVCVP"
     gene            503..1852
                     /locus_tag="VNZ06_06785"
     CDS             503..1852
                     /locus_tag="VNZ06_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HWW20493.1"
                     /translation="MKDIDPKALFRLSVLGPLVSRERLERGELQQIIRQLAQQEYAIP
                     GSRRRHIGEKTLQSWYYAWKQDGLAGLCGKPRVDRGQSKLPPAVQTAILAAKRENPRR
                     SIRQIRLLLESAGIVAHDTLSRCAVHRLLQTHGLSRIVGSASLPEEKRSFVAEHAGSI
                     WYGDVMHGPQLSLGGRRTRTYLVSLMDDASRLIAHSAFCFSETAIDIEGVLKQALLKR
                     GVPRMLIVDNGAAYRASTLQGICARLSIRLVYCRPYAPEGKGKLERWHRSLRDQFLSE
                     LDTTHIHDLADLNARLWAWVEQIYHRTAHSGLGNQTPLARYQQDLPRIRTLGTRATQL
                     DELFLHRVARYVRRDGTVSFQGKRFEVPYELVGKTVLLVVDPHTGTVKGVESEAGEPL
                     GAATLLDAVGNRTRKRRKSQPETVEPAVTGHDAPIENAAHGPNLVELAYQRHYAREL"
     gene            1854..2648
                     /locus_tag="VNZ06_06790"
     CDS             1854..2648
                     /locus_tag="VNZ06_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW20494.1"
                     /translation="MYRQHFGLRCAPLSKEHSELWDDGALAQFNERFQWLLHSPGIGL
                     LTGEPGVGKTAALRHAALALNPHRYQVIYLAETEFGRIDLYRSLARALGLEPAYRRAD
                     LWRDLKLRILELVESKQLLPVWIIDEAQNLPLEFFRDFPAFLNFAFDSRDLLTVWLAG
                     HPSLGQTLERAPYAALYGRIQARVQLKPVIERERFALFIQHALKSAGCSHTLLAESGL
                     ELLRQASRGLPRQAGRILRTAMQMAVPKGLNHLPDELLQQAIEELK"
     gene            2645..2845
                     /locus_tag="VNZ06_06795"
     CDS             2645..2845
                     /locus_tag="VNZ06_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20495.1"
                     /translation="MTPEFDTLFPEGIPDSCARALTDFLYRLALVCEQRYEHELRRDS
                     DKRYRETMDPDQPWRRKTDPAI"
BASE COUNT          567 a         1034 c          900 g          603 t
ORIGIN      
        1 gccctcaatt ctgttcccat tttgcgtttt cgctgccgaa cctgcagccg cacgtgctcg
       61 cgtctgccgt tgtgcgtcgc gccgcgccgc tggtacagct gggcgctgca acaacaggtt
      121 ttgctcctgc tgctcgccgg ctgctcgttg cgccgggcaa gttcgatctt cgcgctcggc
      181 cgacacaccg tacagcgttg gcggcaatgg ctcgatgaac gcagcctggc gttcgaattc
      241 tttttgcgca gccgcttcgc cgaactcggg cgcgcagtcg atcggcctgc attctggctg
      301 ggctgcctgc agcgcatgcc gctgtgtgag gccatggcct ggctcgacgg cgatggtgtc
      361 tgcgtcccgt gatgaactga tcctcgccgg gtacgcgccg gcgcaattct ggtcacaccg
      421 ggctctccac acactttgcc cactcgcgcc gaattcggtt cgcggttctc atggcactcc
      481 ctttttattg ataggagtgt gcatgaagga catcgacccg aaggccctgt ttcgcctgtc
      541 cgtactgggt ccgctcgtca gtcgcgaacg gcttgagcgc ggtgagctgc agcagatcat
      601 ccggcaactg gcgcagcagg agtatgcgat tccgggttct cgccggcgcc acattggcga
      661 gaagacgctt cagtcctggt actacgcctg gaagcaggac ggcctcgccg gcctgtgcgg
      721 caagccgcgc gttgaccgtg ggcagtcgaa actgccacct gcggtgcaaa ctgcgatcct
      781 cgcggccaaa cgcgagaatc cgcgccgctc gatccgccag attcgtctgc tgctcgagtc
      841 cgccggcatt gtggcgcacg acacgctatc gcgctgcgcc gtgcatcgcc tgctgcagac
      901 ccacggactg tcgcgcatcg ttggctcggc aagcctgccc gaggagaagc gtagctttgt
      961 cgccgaacat gccggttcca tctggtacgg cgacgtcatg cacggcccgc agttgtcgct
     1021 cggtggccgg cgcaccagga cctatctggt ctcgctgatg gatgacgcct cgcggctcat
     1081 cgcgcacagc gccttctgtt tctcggagac cgccatcgac atcgagggcg tgctcaagca
     1141 ggcgcttctt aaacgcggcg tgccacgcat gctaatcgtc gacaatggcg cggcctaccg
     1201 cgcttcgacg ctgcaaggca tctgcgcacg cctgtcgatc agactggtct attgcaggcc
     1261 atatgcgcct gaaggaaagg gcaagcttga acgctggcat cgaagtctac gggaccagtt
     1321 cctgagcgag ctcgacacca cgcacattca cgatcttgcc gacctcaacg cgcgcctgtg
     1381 ggcgtgggtc gagcagatct accaccgcac cgcgcacagc ggtctgggca accagacgcc
     1441 gctggcccgc tatcagcagg acctgcccag gatccgcacg ctcggcacgc gagcgacgca
     1501 gctcgacgaa ctgttcctgc accgcgtcgc tcgctacgtg cgccgcgacg gcaccgtgtc
     1561 atttcagggc aagcgcttcg aagtgccata cgagctggtc ggcaagaccg tgttgctcgt
     1621 ggtggatccc catacgggaa ccgtcaaggg cgtagaaagt gaggcgggcg agcccttggg
     1681 cgccgcaacc ctgctcgacg ctgtcggcaa tcgcacacgc aagcggcgca aatctcagcc
     1741 tgagaccgtc gagccggccg tgacaggaca cgacgcgccg atcgagaacg cagcgcacgg
     1801 tcccaatctt gtcgaactcg cctatcaacg ccactacgcc cgggagctgt gacatgtatc
     1861 gacaacactt cggtctacgt tgcgcgccgc tctccaaaga acatagtgag ttgtgggacg
     1921 acggcgcctt ggcgcaattt aacgaacgct tccagtggct actgcacagt cccggtattg
     1981 gcttgctcac gggcgagccc ggcgtgggca agactgccgc gttacgccac gcagcacttg
     2041 ccttgaatcc ccatcgatat caggtcatct atctggctga aaccgagttc gggcgcatcg
     2101 atctgtaccg cagcctagct cgcgcgctgg gtctggagcc cgcctatcgg cgtgccgacc
     2161 tgtggcggga tctgaagctg cgcatcctgg agctcgtcga gagcaaacag ttgttgcccg
     2221 tgtggattat cgacgaagcg cagaacctgc ccctcgaatt cttccgcgac ttcccggcct
     2281 tcctgaactt cgccttcgat tcgcgcgacc tgctcactgt ctggctcgca ggccatccga
     2341 gtctgggcca aacgctcgag cgtgcgccgt acgcagcttt atatggacgt atccaggcac
     2401 gcgttcaact caaacccgtg atcgaacgcg agcgcttcgc cctgttcatc caacacgcat
     2461 tgaagagcgc cggctgcagt cacacgttgc tggccgaatc tggcctggag ttacttcgcc
     2521 aggcctctcg cggcttgccc cggcaagcgg ggcggattct gcgcaccgct atgcagatgg
     2581 ccgtgcccaa aggactgaac catctacccg acgaactgct acaacaggca atcgaggagt
     2641 tgaaatgacg cccgagtttg acacgctgtt ccccgagggt atccccgatt cttgtgctcg
     2701 tgctctgacc gacttcctct accgcctcgc ccttgtatgc gaacaacgat acgagcacga
     2761 actgcgtcgc gactccgaca aacgctatcg cgagacaatg gatcccgatc aaccctggcg
     2821 ccgtaaaacc gaccctgcaa tctagcgccg ccgtcgcgca ctaaacgagg tccattccat
     2881 tcctcattta gtgcgtgacg cccctcgcaa tctcacgcca catagctcgc gacgacaacc
     2941 ctaatttagg ccgggacgta acaccaacac acaggagatc ggaccgccct ggggagtacc
     3001 ctcctcagtg cgaatgatta ctccgttatg catcgcgcca gcgctgagcc acttaccaat
     3061 caagctcaga atgactgggt ccgcgatgcg ctgggccacc atct
//
LOCUS       DATMRM010000232         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2918917, whole genome shotgun sequence.
ACCESSION   DATMRM010000232 DATMRM010000000
VERSION     DATMRM010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2918917"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..829
                     /locus_tag="VNZ06_06800"
     CDS             182..829
                     /locus_tag="VNZ06_06800"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWW20496.1"
                     /translation="MTIDSDQMFLVPHSATHGLSQMVLRTDVAGMPLEWIDYKEAARL
                     YHQDQIAYTCGSLLYRLYGGVNAISGRRTVVEVSSIIATLGHTGNPGNTRHDYVPPLN
                     NETLFRRDAYLCMYCAGRFSSRELSRDHVRPFSQGGLDVWTNVVAACRHCNNEKAWRT
                     PEQAKMQLIAVPFTPTYAEYIFLKGRRVLADQMEYLLAHFPRSSPLHDRIARWLS"
     gene            849..1739
                     /locus_tag="VNZ06_06805"
     CDS             849..1739
                     /locus_tag="VNZ06_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HWW20497.1"
                     /translation="MVYLIDASVYVFRAWHSLPPDIIDRDGNPTRAVYGFARFLTDLL
                     ERRRPQYVAVAFDESHGGSFRHKLYPAYKANREAAPEELKRQFALCREFCRHLGIAEF
                     ASQEYEADDLIGTLAQLCRQQGLRITVVSRDKDLTQLLGPEDVFWDYSDAAEYRYHEI
                     AERFGALPERMADYLALRGDSVDNVPGVPGVGPKTAATLMHLFASLDELFERLDLVAG
                     LPVRGAAKLKGRLLEARSAAYRARALTRIACDVPIDAVRADLLCRAPNLERIASFCDC
                     HGFGNLLRRQAERLVAARAA"
     gene            complement(1802..2134)
                     /locus_tag="VNZ06_06810"
     CDS             complement(1802..2134)
                     /locus_tag="VNZ06_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HWW20498.1"
                     /translation="MSETNSFSRRDALKGFVLLCGAGALGAERSVQGAELPHLSAQDP
                     TAKALGFTEDAKTVDPKKYPTYKTGQTCSNCQQLQGSPGDTYRPCNVFAGKAVNSNGW
                     CQVWVKKA"
     gene            2293..>2481
                     /locus_tag="VNZ06_06815"
     CDS             2293..>2481
                     /locus_tag="VNZ06_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20499.1"
                     /translation="MTKKSEKAKHIESVLNEKRSFEPSAEFTDSANIKASALARMHQR
                     AHQDYLGFWGDLARHSLHW"
BASE COUNT          468 a          741 c          747 g          525 t
ORIGIN      
        1 gtctcccccg gcacgcgctc gcgcagcgcg ccaatcgtaa ctggcatagt gttctcccct
       61 accttaaggc gcgaccgctt ccctggggtg tgcacgtttg gactgtgcgc acgttcaaat
      121 ccttatgcgt tgaattctcg cataatcctg ccgtatctta cagttcatga gaggcgattt
      181 ggtgaccatc gattcagatc agatgttcct ggtaccgcat tctgcgacgc atggcttgtc
      241 gcagatggtg ctgcgaacgg acgtcgccgg tatgccattg gaatggatcg actacaagga
      301 agccgcccgc ctctatcacc aggatcagat cgcttacacc tgcgggtcgc tgctctatcg
      361 actgtatggg ggtgttaacg ccatcagtgg ccgtcgcacc gtagtggaag tgagctcgat
      421 cattgcgacc ctgggacata ccggcaatcc gggtaatacc cgtcacgatt acgtcccgcc
      481 gctcaacaac gaaaccctgt tccgtcgcga tgcgtatctg tgcatgtact gcgccggacg
      541 cttctccagc cgcgaattgt cgcgcgatca tgtgcgcccg ttcagccagg gcgggctgga
      601 tgtgtggacg aacgtcgttg ccgcctgtcg tcactgcaac aacgagaagg cttggcggac
      661 gccggagcag gcgaaaatgc agttgattgc cgtgccattt acgccgacct acgcagagta
      721 catctttctg aagggcaggc gcgtgctcgc ggatcaaatg gagtacctgc tggcacactt
      781 cccgcgcagc agcccgctgc atgatcgtat tgcgcgctgg ttgagttagg gcgtagcctt
      841 aaagcttagt ggtctacctg attgatgctt cggtttatgt ctttcgcgcc tggcactcac
      901 tgccgccgga tatcatcgat cgggatggaa atccgacccg cgccgtctac ggatttgcgc
      961 gtttcctgac cgatctgctc gagcggcgtc ggccgcagta cgtcgcggtc gcgtttgacg
     1021 aaagtcatgg cggttcgttc cgccacaagc tctatccggc ttacaaagcc aatcgggaag
     1081 cggcgccgga agaactcaag cgccagtttg cgctctgccg cgagttctgt cgtcacctag
     1141 ggatagccga gtttgcgagc caggaatacg aggccgacga tctgatcggc acgctggccc
     1201 agctctgccg gcagcagggc ctgcgcatca cggtggtatc cagggacaag gacctcacac
     1261 agctattggg acccgaagac gtgttctggg actacagcga cgccgccgaa taccgctatc
     1321 acgagatcgc tgaacgcttc ggcgccttgc cggagcgcat ggccgactac ctcgcattgc
     1381 gcggtgacag tgtggacaac gtccctggcg tgcccggcgt gggacccaag acggctgcaa
     1441 ccttgatgca tctattcgcg tcgctggacg agctgttcga acggctggac ctggtcgctg
     1501 gcctgccggt gcgcggcgct gccaagctga agggccgact actggaggcg agaagcgcgg
     1561 cctatcgcgc tcgtgcgctg acccgcatcg cctgtgatgt gccgatcgat gcggtgcgcg
     1621 ccgacctgtt gtgccgcgct cccaatctgg agcgcatcgc atcgttctgc gactgccatg
     1681 gcttcggcaa cctgctgcgg cgacaggccg aacgcctggt cgccgcacgg gctgcatgag
     1741 accgctccaa gctctcaagg actgcgcgtg tcgttacgtc ctgctccctt gatgttgcgt
     1801 tttaggcctt cttgacccag acctgacacc agccgttgga gttcaccgcc ttgccggcga
     1861 agacattgca aggccgatag gtatcacccg ggctgccctg cagctgttga cagttgctgc
     1921 acgtctgccc cgtcttgtag gtcggatatt tcttggggtc gacggttttc gcgtcctcgg
     1981 tgaagccgag cgctttcgcg gttggatcct gcgcggacag atgcggcaac tccgcaccct
     2041 gcacgctgcg ctcagcgccc agggcgccgg caccgcacag caagacaaaa cccttcagcg
     2101 catcgcgacg tgagaatgag ttggtttcgc tcatggatcg atcctttcgc cggaatcggg
     2161 gattggcagt gagtgccgga ctttcggcgg cagtggttaa tggtgtggac attttaatgt
     2221 ggaaaatggc tatgaagcga tttcattcga ttgaggtatc ctcacaatca agagcaaggg
     2281 gtaacggcca ccatgacgaa gaagagcgag aaggcaaaac acatcgagtc tgtcctcaac
     2341 gagaagcgaa gctttgagcc gtccgccgag ttcactgaca gcgcaaacat caaggcttca
     2401 gcgctggcac ggatgcatca gcgcgcgcac caggactatc tcggattctg gggggatctc
     2461 gcacgacatt ccctgcactg g
//
LOCUS       DATMRM010000233         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1376292, whole genome shotgun sequence.
ACCESSION   DATMRM010000233 DATMRM010000000
VERSION     DATMRM010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1376292"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..433)
                     /locus_tag="VNZ06_06820"
     CDS             complement(17..433)
                     /locus_tag="VNZ06_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20500.1"
                     /translation="MNWIVAGLIAGVVAWLVDWLMWGKIFTKGMDLFMVQMSPEEMKK
                     FMMPAMVKSFLLALVFGVILANFYGQFKSDLWVQPGGPLAGMEFGAVLWLPTIALATV
                     GSGIWYIKARPLQMATFWAWLARLLAAGLAVGLLMK"
     gene            complement(459..1862)
                     /locus_tag="VNZ06_06825"
     CDS             complement(459..1862)
                     /locus_tag="VNZ06_06825"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HWW20501.1"
                     /translation="MATRLEQDPLGELPVPAEALYGVQTERARQNFPISHLLPLAPFV
                     DAVIRIKKAAALTHKETGRLEAKLADAIVRAADEILGGQHRDQFVVDPYQAGAGTSHH
                     MNANEVLANRANELLGQPRGVYRPIHANDHVNMAQSTNDVIPTAIRLATLAQLPDLLA
                     ALDHLAQAFLAQGKAFDDILKAGRTHLQDATPIRLGQEFTAYGHTIERSRARIAQAAD
                     ALRDLGIGGTAVGTGLNAEPRYPELMVRHLGAMMHTPLRVGTDRVQLMQSMGDATAFS
                     GALRTYAVDLSKIASDIRLLASGPRTGIAEILLPPVQPGSSIMPGKVNPSIAEMVNMV
                     CFQAIGNDVTVATAAEAGQLELNVMMPVIAHNVLFTMLILTNASRVFADACVAGIAAD
                     RAACEHWLERSPALVTALAPKIGYAAAAKLAKEAVAKNVTVRQLVIEKGILKGKELDE
                     VLDLRAMTELGVPGERR"
     gene            complement(1862..>2141)
                     /locus_tag="VNZ06_06830"
     CDS             complement(1862..>2141)
                     /locus_tag="VNZ06_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20502.1"
                     /translation="GTMFFGALVLIHAPLALPLPAGGGGIEVAFLSGFAGDFGHRQVW
                     MLVAWRFYTSFVLTALGVYAMIRRLGVVAARKLVTFGWFKGSKKRIMS"
BASE COUNT          369 a          729 c          686 g          357 t
ORIGIN      
        1 gagcgttcct gtagcgctat ttcatcaaga ggccgaccgc caggccggca gccaacaagc
       61 gggccaacca cgcccaaaag gtcgccatct gcagcgggcg cgccttgatg taccaaatcc
      121 cgctgccgac cgtcgccagc gcgatggtcg gcaaccacag cacggcgccg aactccatgc
      181 cggcaagcgg tccgcccggc tgcacccaga gatcgctctt gaactgtccg tagaagttcg
      241 ccaggatcac accgaacacg agcgccagaa ggaaggattt gaccatcgcc ggcatcatga
      301 atttcttcat ctcctcaggg ctcatctgca ccatgaacag gtccatgccc ttcgtgaaga
      361 tcttgcccca catcagccaa tccacgagcc acgccacgac gcctgcgatc aatcccgcca
      421 cgatccagtt catgcagcgc tccctgtgtg gtgtgtggtt accgccgctc gcccggcacg
      481 cccagctccg tcatggcgcg caggtccagc acctcgtcca gctccttccc tttgagaatg
      541 cccttctcta tcaccagctg ccgcaccgtc acgttcttgg cgaccgcttc cttcgcgagt
      601 ttggccgcgg cggcgtagcc gatcttgggc gcgagcgccg tgaccagcgc cggactgcgc
      661 tcgagccagt gctcgcacgc ggcgcggtcc gccgcgatgc cagcgacgca agcgtcggcg
      721 aagacgcggc tcgcgttcgt gagaatcagc atcgtgaaca gaacattgtg ggcgatcacc
      781 ggcatcatca cgttcagctc gagctgcccc gcttcggcgg cggtggcgac cgtgacatcg
      841 ttcccgatcg cctggaagca gaccatgttc accatctccg cgatcgacgg attcactttg
      901 cccggcatga tgctcgaacc cggctgtacg ggcggcagca agatctcggc gatgccggtg
      961 cggggacccg aggccagcag acggatgtcg ctcgcgatct tgctcaagtc gacggcgtag
     1021 gtgcgcagcg cccccgagaa ggcggtggcg tcgcccatgg actgcatcag ctgaacgcgg
     1081 tcggtcccca cccgcagcgg cgtgtgcatc atcgcgccga ggtgtcgcac catcagctcg
     1141 gggtaacgcg gctcggcgtt gagccccgtg ccgaccgccg tgcccccgat gccgagatcg
     1201 cgcagcgcgt ccgccgcctg ggcgatgcgg gcgcggctgc gctcgatggt atgcccgtaa
     1261 gcagtgaatt cctggcccag ccggatcggc gtggcgtcct gcaggtgcgt gcggcccgct
     1321 ttgaggatgt catcgaacgc tttgccctgg gcgagaaatg cctgggccag gtggtcgagg
     1381 gcagcgagca ggtccgggag ctgggcgagg gtggcgaggc ggatggcggt ggggatcacg
     1441 tcgttggtgg actgcgccat gttgacgtga tcgttggcgt gaatcggcct gtacaccccg
     1501 cgcggttgcc ccagcagctc gttggcgcgg ttcgccagca cctcattggc gttcatgtgg
     1561 tgcgacgtgc cggcgcctgc ctgatacgga tcgaccacga actggtcgcg gtgctggccc
     1621 cccaggatct cgtcggcggc ccgcacgatc gcgtcggcga gcttggcctc gagccgcccc
     1681 gtctccttgt gggtcagggc ggccgccttc ttgatgcgga tcaccgcgtc cacgaagggc
     1741 gccagaggta acagatggga gatggggaag ttctgccgtg cccgctcggt ttggacgcca
     1801 tacaaagctt ctgccgggac cggcagctcg ccgaggggat cctgctccag ccgggtggcc
     1861 atcacgacat tatacgcttc ttcgagccct tgaaccaccc gaaggtgacg agtttgcgcg
     1921 ccgcgacgac acccaacctc cggatcatcg cgtagacccc gagcgcagtg agcacgaagg
     1981 acgtgtaaaa ccgccacgcg acgagcatcc acacctggcg gtggccgaaa tcgcccgcga
     2041 agcccgacag gaatgcgact tcgatgccac ccccgccggc cggcagcggc agcgcgagtg
     2101 gcgcgtgaat cagcaccaac gcgccgaaaa acatcgtgcc g
//
LOCUS       DATMRM010000234         7676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3525912, whole genome shotgun sequence.
ACCESSION   DATMRM010000234 DATMRM010000000
VERSION     DATMRM010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3525912"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VNZ06_06835"
     CDS             <1..326
                     /locus_tag="VNZ06_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20503.1"
                     /translation="SASTAAVGHTIQSYLWTTDPSTSDQLINANQAIATLVVPSFRSI
                     TVILTITDDAGTPTSASQLIESEIAAAAGHTGGAFEPLWLLLLALLALWQVHRRWAGP
                     GKLLN"
     gene            408..2510
                     /locus_tag="VNZ06_06840"
     CDS             408..2510
                     /locus_tag="VNZ06_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20504.1"
                     /translation="MTAVRLRKPQALGRAAICLLLIGLVSLAACSNTNRAVTGSVGVG
                     NGVALLTAGDLTTLYVGQTLQITAAVTNDPGNRGVTWSVQPNNGSAGTLTQITPTSVV
                     LVAPSTVTGQVGATITATANVNSVYASSVTIITNGTPVPNPVQLFPANVNVAYAASFT
                     VSGGVPPFTWALDASSGPLPPGIALLGSTTGLDTISGTPTTANTYPGIVLVATDANSV
                     VARFPAVTLTVNAQNTCVLQGTYALMASGFRGGGGMTHVAHIVINSTGQITGEQDYKD
                     GHRTTLHEQLLSTSNCINRQTNSGQITLNAPSGALLYNVSTTPTDANGVTQSARLQLV
                     GSGDDTASGLLSNVDPSAISAAPPTGSFAFGLLGVEKQEPVGVHFATAGQFTTDSSGT
                     ISTGSIDSNNPAAALNAASLTGTLSAPDSSGRGTASFTALGQTSQYVYYIVRANKMLL
                     MNIDLQTATSPRASGFMTPQVGNVSANSFDATALASPSILSLWGAIVGMEPITSQAMG
                     RLSGGNGSTLSALLDLSDQQTTVIADLTQPLTNQPYTVAANGRGTLTLNNGTQNLNLV
                     FYLDGASDGYVVQQNTTDGSGGLLEAQYPMPATGGFPSSLPGYFVGGTQFAMAAGPIT
                     LNPLATLAFGSLSSNFTNATFYIDPGSGRGLGTLTQTGVGVQPAALYIVSPTKIDVLR
                     FGTRGVDGNIDWLIQDVQ"
     gene            2520..3176
                     /gene="betI"
                     /locus_tag="VNZ06_06845"
     CDS             2520..3176
                     /gene="betI"
                     /locus_tag="VNZ06_06845"
                     /inference="COORDINATES: protein motif:HMM:NF001978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HWW20505.1"
                     /translation="MKAPKYRRVSAAARRQSLIEATLKCLRRHGHDGVSVRRIGAMAG
                     VSPGLINHHFPSTSTLIAAAYQTLARSLLDSIRRYAQDTSADPRVRLRRFFQASFAPE
                     LLDPQLFASWLVFWSMAAHSSAVRRVHDRTYAEYRATLESLLRELRRSSKPVFGVRQA
                     AIGLTALLDGLWLEASLNARTFRPADAVSLCEHWVDALCAGARPRLRTTASGGRKSGG
                     "
     gene            complement(3136..4434)
                     /locus_tag="VNZ06_06850"
     CDS             complement(3136..4434)
                     /locus_tag="VNZ06_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWW20506.1"
                     /translation="MKRGRKLGPLLATALVASNMIGSGIFLLPATLAAVGSISVFGWL
                     IGAAGALLVAAVLARLAQVIPAAGGPCSYAANAFGPAVGFQANAMYWLSCVTGVIAIA
                     VAAVGYLESFFPSLNAPLPGAIATAVLIWLLTLLNILGARLVSQFESVTILIGLIPIL
                     LVATLGWSHFDRQVFLASWNVSHEPTYQAVPESLVLLFWAFTGMESVSVASAVVANPS
                     RNVPIATLGGVAIAAVVYITACTAIMGLIPAAQLARSTAPFADAVSVMFGRVTGGLIA
                     LAALTKSIGTLGGWVLMTAQTSKAAADRGLFPRLFARADDQGVPVLNLLWMAVLMSVI
                     VFVTIAPTLGEQFGKLIAISTILCLLVYVYACLALLRYRRWVPSGGALRAYRPVALAA
                     MVFCAVVIGYADAPLLLWTLAVMLVSAIIHRIFGRRWQLS"
     gene            4470..5585
                     /locus_tag="VNZ06_06855"
     CDS             4470..5585
                     /locus_tag="VNZ06_06855"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HWW20507.1"
                     /translation="MPDLDATSLPAWIYRDQRFFELERAAIFRPSWQLVCHQNDIPEP
                     GDYHCLDLLGESMVSLRGADRTIRSFHNVCRHRAARLLDGPRGSCASRIACPYHQWTY
                     ALDGRLIGVPQRSSFPGLQMSEHALVPLEQEIFLGFVFVRLQPGLPTVRDMAAPYLRE
                     LQDYRMEQLVPQGRVTLRPRAVNWKNVADNYADGLHISVAHPGLSRLFGPGYGIEARE
                     WIDKMWGSVLEKPSCNWSERHYQRLLPSVEWLPPQKQRLWLYLKLWPNLAFDIYPDQI
                     DFMQFLPISPTETLIREIAYVHPDARREMRAARYLNWRINRQVNREDKALIERVQTGM
                     TSGSYSSGPLGESEVCLRSFARRVRSLIPESRQAQPN"
     gene            5586..7220
                     /locus_tag="VNZ06_06860"
     CDS             5586..7220
                     /locus_tag="VNZ06_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWW20508.1"
                     /translation="MASDSSHRSDALIIGGGHNGLVCAAYLAAGGLSVRVLERRGVVG
                     GAAVTEEFFPGFRNSVAAYTVSLLNPKVILDLDLPAHGLRIVERRLSNFLPTEDGRYL
                     CVGAGRTPSEVAKFSARDAGRLSAYAQQLERIAEVLRQLVLQTPPNVIDGSWRQALPE
                     LLRAASIGRSVRKLDMSLRRELLRLMASSAGEYLDGWFESDPIKAVYGFDGIVGNYAS
                     PYTPGSAYVLLHHVFGEVNGKKGVWGHAIGGMGAITQAMARAAAARGVNIRVNAPVQK
                     LLVENGRAVGAVTPGGEEFRASAVISNLNPKLLYLQLLEPTVLPPDFRQRIEQYRCGS
                     GTFRMNVALSELPDFRCLAGRQSADHHTAGIIIAPTLTYMERAYFDARTGGWSAQPIV
                     EMLIPSTLDETLAPKGQHVASLFCQHVAPQLPNGESWDQHRERVADLMIQTVDRYAPN
                     FRYSVLGRQIMSPLDLERTFGLIGGDIFHGALSLDQLFSARPVLGHGNYRGPLPGLYM
                     CGSGTHPGGGVTGAPGHNSAREVLIDFRRGTLKRAS"
     gene            complement(7238..>7676)
                     /locus_tag="VNZ06_06865"
     CDS             complement(7238..>7676)
                     /locus_tag="VNZ06_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWW20509.1"
                     /translation="ELFDRVVLTNLRGAFAAVRYFRRLLERSDLQGGGVVVNISSIAA
                     TLAMGSNVAYCASKAGMDNMTRSLARALAPKIRVVSVSPGLIDTGFVKGINPASFEQQ
                     AKVTPLQRLGLTREIADAVVAVVTQMTYTTGIIIPVDGGRLLV"
BASE COUNT         1398 a         2612 c         2362 g         1304 t
ORIGIN      
        1 gcagcgccag tacggcggcg gtgggccata ccattcaaag ctatctatgg accacggatc
       61 ccagtaccag cgatcagctc atcaatgcca accaggccat tgcgacgctg gtggtgccct
      121 cattccgctc aatcacggtg atcctgacga tcaccgatga tgccggcacg ccaacctccg
      181 ccagccagct gatcgaatcc gagattgcgg cggcggccgg tcatacgggg ggcgccttcg
      241 agccgctgtg gctgctgctg ctcgcgctgc tcgccctctg gcaagtccat cggcgatggg
      301 ccgggcccgg caagctgttg aactgattca ttggccaact gtcgctatgc taggcggcca
      361 ggctgccggc ccagccagcg gcgttcccga ttcgcaagga gatatcgttg actgctgttc
      421 gtttgcgtaa accgcaggcc ttgggccgcg cggcgatctg cctgctgttg attggtctgg
      481 tatccctggc tgcctgcagc aacaccaacc gcgccgtcac cggttcggtc ggcgtcggca
      541 acggcgtcgc gctgctgacg gcgggcgacc tcaccaccct gtacgtgggt cagactctcc
      601 agatcacggc cgccgtcacc aacgatccgg gcaatcgggg tgtgacctgg tctgtgcagc
      661 ccaataacgg gtcggccggt acgctgaccc agatcacgcc gacctcggtc gtgctggtcg
      721 cgccctccac ggtgaccgga caggtcggcg ccacgatcac cgctacggcc aacgtcaata
      781 gcgtctatgc gtcctcggtc acgatcatca ccaacggtac gccggttccg aatccggtac
      841 agctgttccc ggcgaacgtc aacgtcgcct atgcggccag ctttaccgtt tccggtggcg
      901 tgccgccctt tacctgggcg ctggatgcga gcagcggccc gctgccgccg ggcatcgcac
      961 tgctaggcag taccactggc ctggatacca tatcgggcac gccgacgact gccaatacgt
     1021 atccgggcat tgttctggtc gcgactgacg ccaacagtgt ggtcgcacgc tttccggcgg
     1081 tcactctgac ggtcaacgcc cagaatacct gtgtactgca gggcacctac gcgctgatgg
     1141 cctcgggttt ccgcggcggc ggtggcatga cgcatgtcgc ccacattgtg atcaattcca
     1201 ccggtcaaat taccggcgaa caggactata aggacggcca ccgcaccacc ctccacgagc
     1261 agctgctgag caccagcaat tgcatcaatc gacagaccaa ttccgggcag atcacgctca
     1321 acgcgcccag cggcgcgctg ctgtacaacg tttccaccac gccgacggac gccaatggcg
     1381 tgactcaatc cgcacggctg cagctggttg gttccggcga cgataccgcc tcgggtctgt
     1441 tgtccaacgt cgatcccagt gcgatcagcg ccgcaccgcc caccggcagt ttcgcgttcg
     1501 ggctgctggg tgtggaaaaa caggagcccg tcggggtgca cttcgccacc gccggtcagt
     1561 tcacgaccga tagctccggc acgatcagca ccggctcgat cgattccaac aatccggctg
     1621 ccgccctgaa cgccgcgtcg ctcaccggca ccctgagcgc cccggatagc agcggccgtg
     1681 gcacggcgag cttcactgcg cttggccaga cgtcgcagta cgtctactac atcgtgcgcg
     1741 ccaacaagat gttgctgatg aacatcgatc tgcagaccgc cacctcaccg cgcgcgtccg
     1801 gattcatgac gccgcaggtt ggcaacgtga gcgccaacag ctttgatgcc accgccctgg
     1861 cctcaccctc gatactgagc ctgtggggcg cgatcgtcgg catggaaccg attacttcgc
     1921 aggccatggg ccgactgtcc ggcggcaacg gcagcacgct cagcgcgctg ctcgacctga
     1981 gcgaccagca gaccaccgtc atcgccgacc tgacgcagcc gctgaccaac cagccgtata
     2041 cggtcgctgc caacggccgt ggcacgctca ctctcaataa cggcacacag aacctcaacc
     2101 tggtgttcta cctggacggc gccagcgacg gctatgtcgt gcagcagaac accaccgacg
     2161 gcagcggcgg gctgctcgag gcgcagtatc cgatgccggc caccggcggc ttcccctcct
     2221 cactgcccgg ctacttcgtc ggcggcacgc agttcgcgat ggccgccgga ccgatcacgc
     2281 tcaatcctct ggcgacactc gccttcggca gcctgtccag caatttcacc aacgccacgt
     2341 tctatatcga tcccggcagc ggacgcgggc tgggtaccct gacccaaacc ggcgtcggcg
     2401 ttcagccagc cgccctctac atcgtctcgc cgaccaagat tgacgtgctg cgcttcggca
     2461 cgcgcggcgt ggacggcaac atcgactggc tgattcagga cgtgcaatag tcaagccaaa
     2521 tgaaagcgcc gaagtaccgc cgggtcagcg cggcggcgcg tcgtcaatct ctgatcgagg
     2581 cgacactgaa gtgtctgcgt cgccacggac atgatggcgt gtcggtacgc cgcatcggcg
     2641 ccatggcagg ggtctcgccg gggctcatca atcatcactt ccccagcacc agcacactga
     2701 tcgcggcggc gtaccagacg ctcgcgcgct cactgctgga ctccatccgc cgctacgcgc
     2761 aagacacctc ggccgatccg cgggtacgtc tgcgacgctt ctttcaggct tccttcgctc
     2821 ccgagctgct cgatccgcag ttgtttgcca gctggctggt gttctggagc atggcagccc
     2881 acagcagcgc ggtacgacgc gtgcacgatc gcacttatgc cgaataccgc gcgacgctgg
     2941 agtcgttgct gcgcgagctg cgtcgctcct ccaaacccgt gttcggcgta cgccaggccg
     3001 ccatcggtct caccgcactg ctcgatgggc tgtggctgga agcgagcctc aatgcgcgca
     3061 cctttcgtcc cgccgacgcg gtctcactgt gcgaacactg ggtcgatgca ctgtgcgccg
     3121 gggcgcggcc gcgcctcagg acaactgcca gcggcggccg aaaatccggt ggatgatcgc
     3181 ggacacgagc atcaccgcga gcgtccataa caacagcggc gcgtcggcgt aaccgatcac
     3241 caccgcgcag aacaccatcg ctgcgagcgc caccgggcga taggcgcgca gcgcgccgcc
     3301 cgagggcacc cagcgccggt agcgcagcaa cgccaggcag gcatacacgt acaccagcag
     3361 gcacaggatc gtcgaaatgg cgatcagctt gccaaattgc tcacccagcg tcggcgcgat
     3421 cgtgacaaaa acaatgacgc tcatcaggac cgccatccac agcagattga gcaccggcac
     3481 cccctgatca tcggcgcgag cgaacagacg cgggaacaga ccccgatcag ccgccgcctt
     3541 gctggtctgc gcggtcatca acacccagcc acccaaggta ccgatcgact tggtcagcgc
     3601 cgccagtgcg atcagtccgc cggtcacgcg accgaacatc acactcacgg catcggcgaa
     3661 cggtgcggtc gatcgagcga gctgcgcggc cgggatcagg cccatgatcg cggtgcaggc
     3721 agtaatataa accacggccg cgatcgccac cccaccgagc gtcgcaatcg gcacgttgcg
     3781 tgacggattc gccaccactg ccgaggcgac cgacacgctc tccatcccgg tgaatgccca
     3841 gaacagcagc accagcgatt cgggcacggc ctgataggtc ggctcgtgac tgacgttcca
     3901 cgaagccagg aacacctgac ggtcgaaatg gctccagccc agtgttgcca ccagcaggat
     3961 cggaatcagg ccgatcagaa tcgtcaccga ctcgaactgc gagaccagcc gcgctcccag
     4021 aatgttgagc agggtcagca gccagatcag gaccgcggtg gcgatggcac cgggcagcgg
     4081 cgcattcagc gaaggaaaga agctctccag ataacccacg gcggccaccg cgatcgcgat
     4141 cacgccggtg acacacgaga gccagtacat ggcgttggcc tgaaagccga ccgccggtcc
     4201 aaaagcattc gcagcatagc tgcagggccc accggccgca gggatcacct gcgcgagcct
     4261 ggcgagcacg gccgcgacca gcaaagcgcc ggcggcgccg atcagccagc cgaacaccga
     4321 aatactgccg accgccgcca gcgtggccgg caacagaaag attccggacc cgatcatatt
     4381 gctggccacc aacgccgtgg ccagcaacgg accgagtttg cggccgcgtt tcatcttgtc
     4441 gtacgatcgt ccaacaagga attgatgcca tgcccgatct cgacgccacc agtctgcccg
     4501 cctggatcta tcgcgaccag cgattcttcg agctcgagcg cgcggcgatt ttccggccga
     4561 gctggcagct ggtgtgccac cagaacgaca ttcccgagcc cggcgactat cactgtctgg
     4621 atctcctggg cgaatcgatg gtcagcctgc gtggcgccga tcgcacgatt cgcagttttc
     4681 acaatgtctg ccggcaccgc gcagcgcgcc tgctggacgg cccgcgcggc agctgcgcct
     4741 cgcgcatcgc ctgtccctat catcagtgga cctatgcgct ggacggccgg ctgatcggcg
     4801 tgccgcagcg tagcagcttc ccgggcctgc agatgagtga gcacgctctg gtgccgttgg
     4861 agcaggagat tttcttaggc ttcgtgttcg tgcggctgca gcccggactg ccgacggtgc
     4921 gcgacatggc agcgccttac ctgcgcgagc tgcaggacta tcggatggag caactggtgc
     4981 cgcagggccg cgtcacgctg cgtccgcggg ccgtcaactg gaagaacgtc gccgacaact
     5041 acgccgacgg acttcacatc agcgtcgccc acccggggct ctcccggctg tttggtcccg
     5101 gctacggcat cgaagcgcgg gaatggatcg acaagatgtg gggcagcgtg ctcgaaaagc
     5161 cgtcctgcaa ctggtccgag cggcattatc agcggctgct gccgtcggtc gagtggctgc
     5221 cgccgcagaa gcagcggttg tggctgtact tgaaactctg gcccaacctc gcgttcgaca
     5281 tctatccgga tcagatcgat ttcatgcagt tcctgccgat ttccccgacc gagacgctga
     5341 tccgcgagat cgcttacgtg catccggacg cccgacgcga aatgcgtgcc gcacgctatc
     5401 tgaactggcg catcaaccgg caggtcaatc gggaggacaa agcgctgatc gaacgcgtgc
     5461 agacgggcat gacttccggc agctacagca gcggcccgct cggcgaatcc gaagtgtgcc
     5521 tgcgcagttt cgcgcgccgc gttcgcagcc tcattcccga aagccggcag gcgcagccga
     5581 attagatggc cagcgacagc tctcatcgca gcgacgcgct catcatcggc ggcggccaca
     5641 acggcctggt gtgcgccgcc tatctggccg ccggcggcct gtcagtgcgg gtgctggaac
     5701 ggcgcggcgt ggtgggtggt gcggccgtca ccgaggagtt ttttcccggc tttcgcaatt
     5761 ccgtggccgc ctatacggtc tcgctgctga acccgaaggt catcctggat ctggacctgc
     5821 ccgctcacgg actgcgcatc gtggaacgcc gactgtccaa ttttcttccg accgaggacg
     5881 ggcgttatct gtgcgtgggc gccggacgca cgccatcgga agtcgcgaaa ttctcggccc
     5941 gcgatgccgg gcgcctgagc gcctacgcgc agcaactcga gcgcatcgct gaagtcttaa
     6001 ggcagctggt gctgcagact ccgcccaatg tgatcgacgg ctcgtggcgt caggcactgc
     6061 cggagctgtt gcgcgctgcc agcatcggcc gcagcgtccg caagctcgac atgagcctgc
     6121 gtcgcgaact gctgcggttg atggcgagct ccgcgggcga gtacctggat ggctggtttg
     6181 agagcgatcc gatcaaagcg gtgtacggct tcgatggcat cgtcggcaac tacgcgagcc
     6241 cgtatacccc gggctccgcc tacgtcctgc tgcatcacgt gttcggcgaa gtgaacggca
     6301 agaaaggcgt ctggggtcat gccatcggcg gcatgggcgc gatcacgcag gcgatggcgc
     6361 gcgcggccgc cgcgcgtggc gttaacatcc gggtgaacgc gccggtgcaa aagctgctgg
     6421 tcgagaacgg tcgcgccgtg ggcgccgtca cgccaggcgg cgaggagttt cgcgccagcg
     6481 ccgtcatctc caatctcaat ccgaagctgc tctacctgca gctgctcgag ccgactgtgt
     6541 tgccgccgga ctttcgtcag cgcatcgagc agtaccgctg cggttcaggc accttccgca
     6601 tgaacgtcgc gctgtccgaa ctgccggact tccgttgtct ggcgggtcgc cagagcgccg
     6661 atcatcacac tgcgggcatc atcattgctc cgacgctgac ctacatggag cgcgcctact
     6721 ttgacgctcg caccggcggc tggtcggcgc agccgatcgt tgaaatgctg atcccctcga
     6781 cgctggatga aacgctcgcg cccaagggtc agcacgtcgc gagtctgttc tgccagcacg
     6841 tggcaccgca gctgcccaac ggcgagtcat gggaccagca tcgcgagcgc gtggcggacc
     6901 tgatgattca gacggtggat cgttatgcgc cgaacttccg gtactcggtg ttgggccggc
     6961 agatcatgag tccactggat ctggagcgca ccttcgggct gatcggcggc gacattttcc
     7021 atggcgcgct gagtctggat cagctgtttt cggcacgccc ggtgctcggg catggcaact
     7081 atcgtgggcc gctgccgggc ctgtacatgt gtggttctgg cacgcacccc ggcggtggcg
     7141 tcaccggtgc acccggccac aatagcgcac gcgaagtgct gatcgatttc cgacgcggca
     7201 cgctcaagcg cgcctcatag cgccagcacg cccgcgctca taccagcagg cgccctccat
     7261 ccacggggat gatgatgccg gtcgtgtacg tcatctgcgt caccaccgcc accacggcat
     7321 cggcgatttc cctggtcagt cccaggcgct gcagcggcgt caccttggcc tgctgctcga
     7381 aagaggccgg attgatgcct ttgacgaagc cggtatcgat gagcccgggg gaaaccgaca
     7441 ccacacgaat cttaggggcc agcgctcgcg ccagcgagcg cgtcatgttg tccatgccag
     7501 ccttggaggc gcagtaagcc acgttgctgc ccattgccag cgtcgcggca atcgatgaga
     7561 tgttcaccac gacgccacct ccctgcagat cgctgcgctc gagcaggcga cggaagtagc
     7621 gcacggcggc gaacgctccg cgcagattcg tcaggacgac gcgatcgaac agctcg
//
LOCUS       DATMRM010000235         3890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1066570, whole genome shotgun sequence.
ACCESSION   DATMRM010000235 DATMRM010000000
VERSION     DATMRM010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066570"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /gene="tatA"
                     /locus_tag="VNZ06_06870"
     CDS             complement(<1..170)
                     /gene="tatA"
                     /locus_tag="VNZ06_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWW20510.1"
                     /translation="MDLFAPKHLLVILLIVLLIFGGKKLKSLGSDLGSAFRGFKKAMS
                     DGENESEAKQIQG"
     gene            complement(267..767)
                     /gene="dtd"
                     /locus_tag="VNZ06_06875"
     CDS             complement(267..767)
                     /gene="dtd"
                     /locus_tag="VNZ06_06875"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HWW20511.1"
                     /translation="MKRVQSVRAGSRFYHCMIALLQRATQGRVTVDGQVVAEIGSGLV
                     VLLGVVRGDDVRAADRLLERILNYRVFADPAGRMNLSLRDVSGALLLIPQFTLAAETE
                     RGNRPGFSRAADPVDAAALYHYVLTRARSLHALVAGGRFGAPMQVSLVNDGPVTFWLE
                     SGKQTL"
     gene            819..1445
                     /locus_tag="VNZ06_06880"
     CDS             819..1445
                     /locus_tag="VNZ06_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWW20512.1"
                     /translation="MSGRVALKMMLMLLAATCGAQAHAAGDPAHGKLLSYTCMGCHGI
                     EDYKNVYPTYSVPELRGQHASYLVAALKEYKAGERSHATMHEQAASLSEQEMEDIAAY
                     LAGSELKPTTALVGSPPTKVSQLCVACHGTSGVGVMPDYPTLAGQHADYLTRALEEYK
                     HGDRKNPVMPTFMAGLSDDEIQQIAKYYSEQRPALVTVPRRATFLSEK"
     gene            complement(1456..3330)
                     /locus_tag="VNZ06_06885"
     CDS             complement(1456..3330)
                     /locus_tag="VNZ06_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWW20513.1"
                     /translation="MAYSNVEPKRAFWYALLASFCCNDALADPPANPSSADESILPRY
                     LQSRSAHFVDWLSDGSVLIQTRFGDTEQIHRLRMPLGMREQVTFAVRGIGAAQARPYA
                     NDALVYLEPLHGGQSTRLSLQRLDTHELTALTDGMHRDGAPLWAHDGKHLAFASNRVN
                     SSDEEIYELDTAAPTAVPRLLAGGAGYRWHVYDWSSDDQQMLLGREPSTASESDADAI
                     DAHDRQLYTVNVDSGEINAVTASSGSGKNLLSVAVHARSARFASDGHGLILLTNQAST
                     GPASSTQRLIYMDPHTQEWRALSGNTPYEVERFDQSPDGHYIAYTLSENGASRLMLLD
                     EARKLDLAITQLPPGIIGRFKFDLSSQRLGLSVESARTPADVYVFDLDTHLATAWTQS
                     EVGPINTLGFALPQLLRFPTWDRTGNGPLHELSASVYRASGKPSSTPRPVLIALTGRP
                     GAPCRPGFDPFLQFLVTESGWAVVAPGIRGSSSTGDLREDAVRDVGSLLVWIGLQHEL
                     DRNRVVLWGEGFGGYVALQSLADYGDRLLGAVIAFPPHGSAQGHASAIRHPVLLVQGL
                     NNPAAPSYELTQLGVRLKAEGVEVRSLEVPDEGASFALKSHRDEYHQAAADFLAQILR
                     "
     gene            3359..>3890
                     /gene="rsmG"
                     /locus_tag="VNZ06_06890"
     CDS             3359..>3890
                     /gene="rsmG"
                     /locus_tag="VNZ06_06890"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HWW20514.1"
                     /translation="MTGEQQRLIDEAGAIGVALSAEQAVRALRLLDELSVWNRTYSLT
                     AITTREAMIHSHLLDSLSGYAELNGTRIADVGTGPGFPGLPLALVSPQREFMLIDSVA
                     KKLRFVTHAVRVLELTNVTTQQVRVESLSPAQPYDTVLARAFAALPDLLAAVQGLCGP
                     ATRVIAFKGQYPADELA"
BASE COUNT          747 a         1210 c         1221 g          712 t
ORIGIN      
        1 ccctgaatct gcttggcttc gctctcgttt tcgccgtcgc tcatcgcctt cttgaagcca
       61 cgaaacgcgc tacccaggtc tgatcccagg ctcttcagct tcttaccacc gaagatgagc
      121 aatacgatca agaggatgac gagtaagtgc ttgggggcaa agaggtccat gggcgctccg
      181 gtcattgaag ccttaggggc ttgagtgacc atgataggtc atttggcgac gcataactaa
      241 ctggcagccc agcaatttgt gacgctttag agcgtttgtt tgccgctttc taaccagaac
      301 gtcacaggac cgtcattaac cagcgacacc tgcatagggg cgccgaatcg gccgccagca
      361 accagcgcgt gcaggctgcg tgctcgtgtc aggacatagt ggtagagcgc ggccgcatcg
      421 accggatcgg ccgcccggct gaagccgggt cgattgccgc gctcggtttc cgccgccagc
      481 gtgaattgcg gaatcagcag cagggcgccg ctgacatctc gcagactcag attcatgcgc
      541 ccggccggat cagcgaacac gcgatagttg agaatacgct cgagcaaccg atccgcagcc
      601 cggacatcat cgcccctgac cacacccaac agcacgacca gaccggagcc gatttccgcg
      661 acgacctgac catcgacggt gacacggcct tgcgtcgcgc gctgtagcaa cgcgatcatg
      721 cagtggtaaa agcgcgatcc ggcccgtaca ctttgcaccc gcttcatggc ccgaatgggc
      781 ccattattca tcaacttgac gctggagttg gtatgcctgt gtccggtcgc gttgccctca
      841 agatgatgct gatgctgctg gccgctacgt gcggcgcaca ggctcatgcg gccggagatc
      901 ccgcgcacgg caagctgctg tcatacacct gtatgggctg tcatggcatc gaggactaca
      961 agaacgtcta tccgacgtac agcgtgcccg aactgcgcgg acagcacgcc tcctaccttg
     1021 tcgccgcgtt gaaagaatac aaggccggcg agcgatccca cgcgacgatg cacgaacagg
     1081 cggcctcgct ctccgagcag gaaatggaag acattgcggc ctatctggcg ggcagtgaat
     1141 tgaagccgac caccgcgctg gtgggtagcc cgcccaccaa ggtgtcgcag ctgtgcgtgg
     1201 cctgccacgg caccagcggc gttggcgtga tgcctgacta cccgaccctg gccggacagc
     1261 acgccgatta tctgacgcgt gcgctggaag aatacaagca cggcgatcgg aagaatccgg
     1321 tcatgccgac cttcatggcc ggcctcagtg acgatgagat tcaacagatc gcgaagtact
     1381 actcggaaca gaggccggca ctggtgacgg ttccgaggcg cgcgacgttc ctgtccgaga
     1441 agtagcgcct gcccgttagc gcaggatctg agcaaggaag tctgcggccg cctgatggta
     1501 ctcgtcgcga tgtgatttga gggcaaacga ggccccttcg tccggcacct cgagcgatcg
     1561 tacttcgacg ccctcggctt tgagccgcac gccaagctgc gtcaattcat aggacggggc
     1621 cgctggattg ttcaatccct gtaccagcag caccgggtgt cggatcgctg acgcgtgccc
     1681 ctgtgcggat ccatgcggtg gaaaggcaat gaccgcgccg agcagccgat cgccgtaatc
     1741 cgccagcgac tgcagcgcga cgtagccgcc gaaaccctcc ccccacagca cgacgcgatt
     1801 gcggtccagt tcgtgttgca gtccgatcca taccagcagc gagccgacat cgcgaaccgc
     1861 atcctcgcgc aaatcccccg tactgctcga gccgcggata ccgggggcca ccaccgccca
     1921 cccggattcg gtgaccagga attgcaggaa gggatcgaag ccaggtcggc agggtgcccc
     1981 cgggcggcct gtgagcgcga tcaggaccgg tcgtggagtg gagctgggtt tgccgctcgc
     2041 gcggtatacc gacgcgctaa gttcatgcag cggtccgttg ccggtgcgat cccaggtcgg
     2101 gaagcgaagc agctgtggca gtgcgaagcc cagagtattg atcggcccga cctcactctg
     2161 cgtccaggcg gtcgccaggt gagtgtcgag gtcgaacaca taaacatcgg ccggcgtgcg
     2221 cgctgactcg acactcagac caaggcgctg gctcgacaga tcaaatttga accgaccgat
     2281 gatcccgggc ggcagctgcg tgatggccag atcgagcttg cgcgcttcat cgagcagcat
     2341 cagccggctg gcgccgttct cgctgagggt ataggcgatg tagtgtccgt ccggactctg
     2401 atcgaagcgc tcgacctcgt aaggcgtgtt gccgctcagc gcgcgccatt cctgggtatg
     2461 cggatccatg tagatcaggc gctgtgtgct ggaagcgggg cctgtggatg cctgattcgt
     2521 cagcaggatc aggccatgtc cgtcgctggc gaaacgggca ctgcgcgcat gaaccgcgac
     2581 tgaaaggagg ttcttgccgc tgccgctgct tgccgtcacg gcattgattt caccgctgtc
     2641 cacgttgacg gtatagagct gccgatcatg ggcatcgatc gcgtcagcgt ccgattcgct
     2701 ggcagtgctt ggctctcgtc ccagcaacat ctgctggtca tcgctcgacc agtcgtagac
     2761 atgccagcgg tagccggcgc caccggccag cagccgcggg accgcggtcg gcgccgcggt
     2821 atcgagctcg tagatctcct cgtcgctgct gttgacacgg ttgcttgcaa acgccaggtg
     2881 tttgccatcg tgcgcccaga gtggagcgcc gtcgcgatgc atgccgtcgg tcagcgcggt
     2941 cagttcgtgg gtgtccagcc gctgtaacga cagacgcgtg ctttgacccc cgtgcagcgg
     3001 ctccagatac accagtgcgt cgttcgcata cggccgcgcc tgcgcggcac cgatacctct
     3061 taccgcaaag gtaacctgct cacgcatgcc gagcggcatg cgcaagcgat gaatctgctc
     3121 agtgtcaccg aagcgagttt ggatcagcac actgccgtcc gagagccaat ccacgaaatg
     3181 agccgagcgg gattgcagat agcgaggcag tatgctctcg tcggcgctcg acgggttcgc
     3241 gggggggtcc gcgagcgcgt cattgcaaca aaagctcgca agcagcgcgt accagaaagc
     3301 gcgtttcggt tcgacgttag aatatgccat tgcgctgata tggtgccaga tccgaaacat
     3361 gaccggcgag cagcaacgac tcatcgatga ggcaggtgcc atcggagtgg ctctgagcgc
     3421 ggagcaggcg gtgcgcgcgc tgcgcctgct cgacgaattg agcgtctgga accgtactta
     3481 tagcctaacg gcgatcacga cccgcgaggc gatgatccat tcgcatctgc tcgatagcct
     3541 gagcggctat gcggaactca acggcacgcg gattgccgat gtgggtaccg gccccggatt
     3601 tcccgggctg ccgttggcac tggtgtcgcc gcagcgcgaa tttatgttga ttgactcggt
     3661 cgccaagaag ctgcgattcg tcacgcatgc cgtgcgcgtg ctggaactga cgaacgtcac
     3721 cacccagcag gtgagggtgg agagcctgtc gccggcgcag ccctacgaca cggtattggc
     3781 gcgcgcgttt gccgcactgc ccgacctgtt ggccgcggta cagggcctgt gtggcccggc
     3841 aacccgcgtc atcgctttca aagggcagta tccggccgac gagctggccg
//
LOCUS       DATMRM010000236         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2299367, whole genome shotgun sequence.
ACCESSION   DATMRM010000236 DATMRM010000000
VERSION     DATMRM010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2299367"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1249
                     /gene="cofG"
                     /locus_tag="VNZ06_06895"
     CDS             194..1249
                     /gene="cofG"
                     /locus_tag="VNZ06_06895"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HWW20515.1"
                     /translation="MLAAARALKEKYRAGVITYSRKVFLPLTNLCRDYCGYCTFRRDP
                     DDPGAHTMTPDEVLEVARQGEKLGCTEALFSLGDRPEAIFPGMRATLRRLGYRSTLHY
                     LESMCEMVLRETQLLPHANPGLLSANWIERLRVSNPSLGLMLESTSAQLAAAHPPDKT
                     PDKLPALRLKTIEDAGKLGVPFTTGILIGIGETAADRVDSLLAIKRLHERYGHVQEVI
                     VQNFRAKPRIPMAAWPEPDHEDMLRTLAVARLLLGDMNIQAPPNLTAKGYERLLSGGI
                     NDWGGVSPLTPDFINPEAPWPHLLDLQQRTEAAGQKLVQRLPVYPEYVERVAQRGGLL
                     GKRLRAAVDLQGYARAA"
     gene            1250..2245
                     /gene="cofD"
                     /locus_tag="VNZ06_06900"
     CDS             1250..2245
                     /gene="cofD"
                     /locus_tag="VNZ06_06900"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HWW20516.1"
                     /translation="MIVVFTGGTGGTKLVQGLQQVVAPENLTVMVNTGDDIEWWGLHI
                     SPDIDSVLYGLADLLSKDRGWGVEGDSFRCLDRMKQLGQQGWFSLGDLDLATHLTRTA
                     LLRSGKSLSEATAELAKRFGIRVGVLPMSDQPVSTMLDTSMGTLTFQEYFVRERHQVD
                     VQAVRFEGAEQARPAPGVIESIESAEAIIFAPSNPVTSIGPILAVPGIREALRATNAP
                     VAAVSPIVGGAAVSGPAAQLMQMNEWPASIAGVAQAYEDFLDVLVVDNADQEAISSQQ
                     SAFGRETELRASSHDLRTKSIRFLATNTMMRTLDDKRNLAAFLLEACEVPQSAKA"
BASE COUNT          461 a          647 c          745 g          474 t
ORIGIN      
        1 cggtgagaca tcctatcgct acgtcgatga cagcataatg cttcctgcta ccagcataat
       61 gcggcttcca gccccgacgg cgctctcagc cgaatcggtt cgcgcagctc tgcgagaagt
      121 tcaggacggc gctccgttgt cgccggagat cgcctgccaa ttattgcgct gcgatgacga
      181 catactgccc gacttgctgg ccgcggcccg cgcgctgaaa gagaaatatc gcgcaggcgt
      241 catcacctac tcgcgcaagg tttttcttcc gctcaccaat ctgtgtcgtg attattgcgg
      301 atactgcacc tttcgccgcg acccggacga ccccggtgcg cacaccatga ctcccgatga
      361 agttttagag gttgcaaggc agggcgagaa attgggttgc accgaagcac tgttttcgct
      421 gggcgatcgg ccggaggcga tttttcccgg gatgcgcgcg accctgcgac gactcggtta
      481 tcggagcacg ctgcattatc ttgaatcgat gtgcgaaatg gtgttgcgcg agacgcagct
      541 gctgccgcat gcgaatcccg gtctgctgag cgccaactgg atcgagcgcc tgcgcgtcag
      601 caatccgagc ctggggctga tgctggagag caccagcgcg caacttgcgg cagcgcatcc
      661 gcccgacaaa acgcctgaca agcttccggc gctacgtctg aagacgattg aggatgccgg
      721 caagctcggc gttccgttta ctacgggaat actgattggc atcggggaga cggcggcgga
      781 ccgcgttgat tccctgctgg cgatcaagcg actgcatgag cggtacggac acgtgcagga
      841 ggtcatagtt cagaacttcc gcgcgaagcc gcggattccg atggcggcgt ggcctgagcc
      901 ggatcatgaa gacatgctgc gtacgctcgc ggtagcgcgc ttgctgctcg gagatatgaa
      961 tattcaggcg ccgccgaact tgaccgcaaa aggttatgag cgtttgctct cgggcggaat
     1021 taatgactgg ggcggcgtgt cgccgctgac gccggatttc ataaatcctg aggcgccgtg
     1081 gccgcatctg ctcgatttgc agcagcgcac cgaggccgcc gggcagaagc tggtgcagcg
     1141 gttgccggtt tatccggagt atgtcgagcg ggtggcgcaa cgcggtggac tgctggggaa
     1201 gcggctgcgc gcagcggttg atctgcaagg atatgcgagg gcagcataga tgattgtggt
     1261 tttcacaggc ggcacgggtg gcaccaagct ggtacagggt ttgcagcagg tggttgcgcc
     1321 tgagaacctc accgtcatgg ttaacaccgg cgatgatatt gagtggtggg gactgcacat
     1381 ctcgcccgac atcgactccg tgctctacgg actggctgac ctgctgagta aagatcgcgg
     1441 ctggggggtc gaaggcgaca gctttcgttg tctggatcgc atgaaacagc taggtcagca
     1501 gggatggttc agtctgggcg atcttgattt ggcgacgcac ctgacgcgca ctgcgttgct
     1561 gcgttcgggt aagagcctga gcgaagccac tgcggaactg gcgaaaagat ttggtattcg
     1621 cgtgggtgtg ctacctatgt cggaccaacc cgtgtcgacc atgcttgaca cgtcgatggg
     1681 tacgctgacg ttccaggaat attttgtgcg cgagcgtcac caggtggatg tccaggccgt
     1741 gcgcttcgaa ggcgccgaac aggctcgtcc tgcgcccggc gtcatcgaga gcattgagtc
     1801 ggccgaagcg atcattttcg cacctagtaa tcctgtaacc agcattggac cgattctcgc
     1861 tgtcccggga attcgcgagg cattgcgcgc gacgaatgcg cccgtggccg cggtaagccc
     1921 gattgttggc ggcgcggcgg tttccggacc ggcggcgcag ctgatgcaga tgaacgagtg
     1981 gccggccagc attgcaggcg tggctcaggc gtacgaggat tttcttgatg tgctcgtcgt
     2041 cgacaatgcg gatcaggaag caatcagcag tcagcaatca gcattcggcc gtgaaacaga
     2101 gcttcgagct tcgagccacg atctacggac gaagtcgatc cgatttcttg ccaccaacac
     2161 catgatgcgt actctcgacg acaaacgaaa cctcgcggca tttcttctcg aggcttgcga
     2221 agtgccgcag agtgccaagg catgaagata cgcggaattc tgctgattcc gttcaagagt
     2281 ctggccacag ccaagcaacg tctggcagaa gcgctggacg cacgcca
//
LOCUS       DATMRM010000237         3089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4491625, whole genome shotgun sequence.
ACCESSION   DATMRM010000237 DATMRM010000000
VERSION     DATMRM010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4491625"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3089)
                     /locus_tag="VNZ06_06905"
     CDS             complement(<1..>3089)
                     /locus_tag="VNZ06_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20517.1"
                     /translation="RTLRTTLGSIQVIDPLAGSVITAFPDYIKDPSHNNYAGVLLLQP
                     IVGQVSGTSVDTGTGPIVVSGNLDASCDQDPEEYAFEDWQIVDGVRLVLVAWPSTPST
                     LMLPSVSPAASWRNRLVYTVFNAEMTLAPDDCLPWDLLGVPVGLIGFDSSWKAQFLDL
                     SAVVRAGGLPRSRYVLPAQAGAARLLVQPALAQARVSQLNEQINTLPALTNFVPPFAF
                     LPPCGVVPASAVDFNKKVGAWFPSNWSIEVGPIHQEEIETALLSTMTALPLDVTQNES
                     VEVLVPLPDALYDPKILLTETVDPVFEQAVVSATGERDGVLQHREAIQEEANALYQVL
                     TGMQQAPLYDPDAGLTPAEIIARDAQTYTPAVNETFGTQPSNSGYASTDYQQLLAETA
                     KFPYTLTQDGNGNTLSPPLRLFSADDLNDMAQNGLQHFIDRINRKLARANDLLDLAFL
                     TAQSDIYRFRQYVLGATDATALATSPIVANIASGESAAATAANLRNYLSSVLPPAPSG
                     APPAASGAPPATSTTPPASPAPATTLRSRLMLGRASAVPLRSPTVFTSPVASSTVATS
                     RVNAAALTKLQTPATAGSFAATAAAAAVSRAGATAGVERLPSAGIARLPSAATAGVFE
                     PVAPGTVSEPATTGDILGSSPVVGAQLNLRTLTIAQRLASPPSQVGIFYAVGNRLAIL
                     QLLADLEITLADIPILVDQWPPLPTTTTTPAPTTTAAPVRIVMPTFADFKINSGAVLQ
                     AVSAPNVSMNSDEADLFSTGIHVLEQHTSLLRAVEARIAQYSDFLTQCGTALSNIQTD
                     LPQAQTLVTQLENDLAQARQDLAFTTALLNDETQRVANVNAQRADTLKNVPVVVYTRP
                     RTLVTDADVPSRQLVPGNIASPVPGCLQESLAVPPELREIVALLREAPVNWLPSIEAL
                     LSNLERPSLLQEVAVAAQARATMQLQLPMRVSSAASEPGVYAPVIAGIYSANQQAFRT
                     FQTQRAASQPVALLNQSWTGQLQALQSVIAAGDLQAADAVHAEVVSATSNLMQQISS"
BASE COUNT          564 a          942 c         1001 g          582 t
ORIGIN      
        1 cgctggaaat ctgttgcatc aggttggaag tcgcactgac cacttcagca tgcaccgcgt
       61 cggcagcttg cagatcgccg gccgcaatca cgctttgcaa tgcctgcaat tgtccggtcc
      121 agctctggtt gagtagtgcg accggctgtg acgcggcgcg ttgcgtctgg aaggtgcgaa
      181 aggcttgctg attcgcgctg tagatgccgg cgatcaccgg cgcatagacg cccggttcgg
      241 aggccgccga agaaactcgc atgggcaact gcaactgcat cgtggcgcgc gcttgcgcgg
      301 caaccgcgac ttcctgcagc agactcgggc gctccaaatt gctcagcaat gcctcgatcg
      361 agggcagcca gttcaccggc gcctcgcgca acagcgccac gatctcgcgc agctccggcg
      421 ggacggccaa cgattcttgc aggcatcccg gtacgggact ggctatattg ccgggcacca
      481 gctggcggct gggaacgtcc gcgtccgtca ccagcgtgcg cgggcgcgtg tatacaacta
      541 ccggcacgtt tttcaaagtg tccgcccgct gggcattcac gttggccacc cgctgggttt
      601 cgtcgttcaa cagggcggtg gtgaacgcca gatcctgccg cgcctgggcc agatcgtttt
      661 ccagttgagt gaccaacgtc tgcgcttgtg gcaagtcggt ttgaatattg ctgagtgcag
      721 tgccgcattg cgtcaggaaa tcgctgtact gcgcgatgcg ggcctcgacc gcgcgcagca
      781 gcgatgtgtg ctgctcgaga acgtgaatgc cggttgagaa caggtccgct tcgtccgagt
      841 tcatgctcac attgggcgcg ctgactgcct gcagcacagc gcccgaattt atcttgaagt
      901 cggcgaacgt cggcatgaca atccgcactg gcgccgcagt cgtcgtggga gctggagtgg
      961 tggtcgtcgt cggcaacggc ggccattgat caaccaggat cggtatgtcg gccagggtta
     1021 tctcgagatc ggccagcaat tgcagaatcg ccagccggtt cccgaccgca tagaagatgc
     1081 cgacctgcga aggcgggctg gccaagcgct gtgcgatggt cagcgtgcgt aggttgagct
     1141 gtgcacccac gactggcgaa ctccctaaaa tgtcaccagt ggtagctggc tcactgactg
     1201 ttcccggcgc caccggttcg aagacgcctg ccgtcgccgc cgatggcaag cgtgcgatgc
     1261 ccgctgacgg caagcgctcg acgcccgccg tggcacccgc gcggctaacg gcggccgccg
     1321 ccgcggtcgc cgcgaaactg cctgcggtcg ccggtgtttg caatttcgtc agggccgcag
     1381 cgttcactcg gctggtggcg accgttgacg aggcgactgg cgatgtgaac acggtcggcg
     1441 accgcagtgg caccgcggaa gcgcgtccaa gcatcagtcg ggaacgtagc gtggtagcag
     1501 gggcgggtga cgctggtggc gttgtggagg tggcgggtgg cgcacctgac gcggcgggtg
     1561 gggcgcctga cggggcgggt ggaagaacgg aactcaggta attccgtaga ttcgcagcgg
     1621 tggccgccgc cgattcgcca gaagcgatgt tggccacgat tggagaagtg gccagcgccg
     1681 tcgcgtccgt cgcgcccaac acgtattgcc gaaaccggta aatatcgctt tgcgcggtga
     1741 ggaaggccag atcgagcaga tcgttggccc ttgccagctt gcggttaatc cggtcgatga
     1801 agtgctggag gccgttctgg gccatgtcgt tcaagtcgtc cgccgagaat agcctcagcg
     1861 gaggcgacaa cgtgttcccg ttaccgtcct gcgtgagcgt gtacggaaac tttgcagtct
     1921 ctgcgagcag ttgctggtag tccgtagacg catagccgct gttggatggc tgggtaccga
     1981 aagtctcatt gacagcaggc gtataagtct gcgcgtcgcg cgcgataatt tccgcgggcg
     2041 tcagtcccgc gtccggatca tacaacggcg cctgctgcat gcccgtcagc acctggtaaa
     2101 gcgcgttggc ctcctcctgg atcgcctcgc gatgttgcaa gacgccgtcg cgctcccctg
     2161 tggcgctaac caccgcctgc tcaaacaccg gatcgaccgt ttccgtaagc aggattttgg
     2221 ggtcgtagag cgcgtcgggc agcggcacca gaacctcgac tgattcattc tgcgtcacgt
     2281 cgagcggcag cgccgtcatc gtggacagta gcgctgtttc aatctcttcc tggtggatcg
     2341 ggccaacctc gatactccag ttggaaggaa accacgcgcc cacctttttg ttaaaatcca
     2401 cggcggaggc gggcaccacg ccgcaaggcg gcaggaatgc aaagggcggg acgaagttag
     2461 tcaacgcggg cagcgtgttg atctgttcgt tgagttggga gacacgtgcc tgcgccaacg
     2521 ccggttggac cagtagccgt gcggcgccag cttgcgccgg caacacgtaa cggctgcgcg
     2581 gcaagccgcc ggctcgtacc accgccgaca gatccagaaa ctgggccttc caactgctgt
     2641 cgaatccgat cagccccacc ggcacgccga gcaggtccca tggcagacag tcgtccggtg
     2701 ccagcgtcat ctctgcattg aatacggtgt agaccagtcg gtttctccaa ctcgccgccg
     2761 ggctgacgga gggtagcatg agcgtactgg gggtggatgg ccacgccacc agcaccaggc
     2821 gcacgccatc cacgatctgc cagtcttcaa acgcgtactc ctcggggtct tgatcgcatg
     2881 aagcatccaa gttgcccgac acaacaatag gcccagtacc ggtatcgaca cttgtcccgc
     2941 tgacctggcc gacgatcggc tgcaacagga gcacgccagc gtagttgttg tgactcggat
     3001 ctttgatgta atccgggaac gccgtgatca ccgaccccgc cagtggatcg atcacctgaa
     3061 tcgagccgag cgtagtccgc agcgtccgc
//
LOCUS       DATMRM010000238         2957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4491728, whole genome shotgun sequence.
ACCESSION   DATMRM010000238 DATMRM010000000
VERSION     DATMRM010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4491728"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VNZ06_06910"
     CDS             complement(<1..423)
                     /locus_tag="VNZ06_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HWW20518.1"
                     /translation="MIAVPPESRVAGKSGPLQWLGSLLFTSFFPLWTFFYAIFFVIAC
                     SLLPYRARFELARVWARVILAVLRWSCGLDYRVEGTELIPPGNHVALLKHSSSWETVA
                     QALLLPPQVWVLKRELLWVPVVGWGIRQLRAIAVNRSAG"
     gene            complement(420..2540)
                     /gene="glyS"
                     /locus_tag="VNZ06_06915"
     CDS             complement(420..2540)
                     /gene="glyS"
                     /locus_tag="VNZ06_06915"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HWW20519.1"
                     /translation="MNQPTSARIERRDLLVELGTEELPPKALPTLEQSFAEGMRSRLT
                     QAGLEFGELESFATPRRLAVLVRRLPIRQPDQTIKRRGPPLAQAFDADGAPTRAAAGF
                     AQSNGVSVDALRRERDAKGIEYLSYEGRRSGADTASLLASLVQHSLDALPIPKRMRWG
                     SGEAQFVRPVHWLVMLFGRDVIPGQVLGVASGNLSYGHRFMAPTPIRLSTPAQYAKAL
                     RTRGKVVASFPERRASIRAQVEQLASTLNGRAIIGEELLDEVTGLVEWPLALAGRFEA
                     RFLSLPREVLLSTLQEHQRYFAVEDGSGALLPNFITISNIDSPDASTVRAGNERVIRP
                     RLSDGAFFWEQDRRQPLAARRFDLDRVTFQAQLGSQGARTERIAKLAAAIAAAIGADA
                     QATARAAQLVKCDLLTAMVGEFPELQGIMGRYYALADAEPAVVADTIRDHYRPRGAGD
                     ALPESKVGDALAIADKLDTLAGIFAIGHKPSGTRDPFGLRRAAIGVLRIALEHRLDLD
                     LDDMTSKAVRLQPLPDIEARAGAVAREVIDFMTERLRAQYLERSDKTGITTEMFDAVL
                     AAEPRSLPDFDERLRAMVGFVASPEGASLASANKRIANILRKSDDCVPHVVSDELLRE
                     DAERELHRALKNVRERTLSAVNARQYARGLEMLSTLRPQIDTFFDQVLVNDPDQALRN
                     NRVALLAEVRSLFCHVADMSRLPG"
     gene            complement(2537..>2957)
                     /locus_tag="VNZ06_06920"
     CDS             complement(2537..>2957)
                     /locus_tag="VNZ06_06920"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HWW20520.1"
                     /translation="YLQGVESIFDIVWTDGPLGRVTYGDVFHQNEVEQSRYNFEFANT
                     AVLLQRFDEHEKAANELLEAKLPLPAYEQVLKASHTFNLLDARRAISVTERQRYILRV
                     RKLAGGVARSYYESREALGFPMLQQTASAPVALGAPA"
BASE COUNT          528 a          967 c          951 g          511 t
ORIGIN      
        1 gcccgcgctg cggttgacag cgatcgcgcg cagctgccgg atgccccaac cgacgaccgg
       61 tacccacagc agctcgcgct tcaataccca cacctgcggc ggcagcagca gcgcctgcgc
      121 caccgtctcc cagctcgagg aatgcttcag cagcgcaacg tgattgccgg gcggaatgag
      181 ttcggtcccc tcgacgcggt agtccagtcc gcagctccag cgcaacaccg ccagaatgac
      241 gcgcgcccac acgcgcgcca gctcgaaacg ggcgcggtaa ggcaggagcg agcaggcgat
      301 gacgaaaaag atcgcgtaaa aaaaggtcca cagcgggaag aagctggtaa acagcaagga
      361 gccgagccac tgcaacggac ccgactttcc ggcgacgcgc gattcagggg gtaccgcaat
      421 catcccggca gtcgcgacat atcggcgacg tggcagaaca ggctgcgcac ctccgccagc
      481 agcgcgactc gattgttccg cagtgcctgg tccggatcgt tgaccagcac ctggtcgaag
      541 aacgtatcga tctgcggacg cagtgtggac agcatttcga gtccccgcgc gtactggcgc
      601 gcattgaccg cactgagcgt gcgctcgcgc acgttcttga gtgcgcgatg cagttcgcgt
      661 tcggcatctt cgcgcagcag ctcgtcggac accacgtgag gcacgcagtc atcggacttg
      721 cgcaggatgt tggcgatgcg cttgttggcg gaggcgaggc tggccccttc cgggctggca
      781 acgaagccga ccatcgcccg cagtcgttcg tcaaaatcgg ggagcgagcg tggctcggcg
      841 gccaataccg cgtcgaacat ctccgtggtg atgccggttt tgtcgctgcg ctccagatac
      901 tgggcgcgca ggcgttccgt catgaagtcg atgacctcgc gcgccaccgc gcctgcgcgt
      961 gcctcgatat caggtagcgg ctgcaaacgc acggctttgg acgtcatgtc gtcgagatcc
     1021 agatccagcc gatgctcgag cgcgatgcgc agcaccccga ttgccgcgcg gcgcaagccg
     1081 aacggatcgc gggtaccgct gggcttgtga ccgatcgcga agattccggc gagcgtatcg
     1141 agcttgtcgg caatggccag cgcatccccg actttggatt ccggcagggc gtcgcccgcg
     1201 ccgcgcggtc gatagtgatc gcgaatcgtg tccgctacga ccgcaggttc cgcgtcggct
     1261 agcgcgtagt agcgacccat gatgccctgc agttccggga attcaccgac catcgcagtc
     1321 agcaggtcgc acttcaccag ctgtgctgcg cgcgccgtcg cttgcgcatc ggcaccgatc
     1381 gccgctgcga tcgcggccgc cagttttgcg atgcgctcgg tgcgcgcacc ctgcgatccg
     1441 agctgtgcct gaaaggtgac gcggtcgaga tcgaagcggc gcgcggccag cggctgacgg
     1501 cggtcctgct cccagaagaa agctccgtcc gacagccgtg gacgaatcac gcgttcattg
     1561 ccggcgcgca ccgtcgatgc gtcggggctg tcgatgttgc tgatcgtgat gaagttcggc
     1621 agcagcgcgc cactaccatc ttcaaccgcg aagtagcgtt ggtgttcctg cagtgtggac
     1681 agcaatactt cgcgcggcag cgacaggaat cgcgcctcga aacgcccggc cagtgctagc
     1741 ggccactcga ccagcccagt gacctcatcg agcagctcct cgccgatgat cgcgcgacca
     1801 ttcagcgtgc tcgcgagttg ctccacctgt gcgcggatgc tggcgcgccg ctcgggaaag
     1861 gatgccacta cttttccacg cgtacgcagc gccttcgcat actgggcagg ggtcgatagc
     1921 cggatgggtg tgggcgccat gaagcgatgg ccatagctca gattgccgga agcgactccc
     1981 agtacctgac ctggtatgac gtcgcgcccg aatagcatca ccagccagtg caccggccgc
     2041 acgaactgag cctctcccga gccccagcgc atgcgcttcg ggatcggcag cgcatccaga
     2101 ctgtgttgaa ccagcgacgc gagcagcgat gcggtgtcgg cgccgctgcg acgaccttca
     2161 taactgaggt attcgatgcc cttggcgtcg cgctcgcgcc gcagggcgtc gacgctgaca
     2221 ccattgcttt gcgcaaagcc ggcagccgcg cgcgtcggcg cgccgtcggc atcgaacgcc
     2281 tgcgcgagcg gtgggccacg acgcttgatg gtctgatcgg gttgtcgaat cggcagcctg
     2341 cgtaccagaa cggccaaccg gcgcggcgtc gcaaaggact ccagctcgcc aaactcaagg
     2401 cccgcctggg tcaggcgcga gcgcatgcct tcggcaaagc tctgctcgag tgtcggtagc
     2461 gccttgggcg gaagttcttc ggtaccgagc tcgaccagaa gatcgcgacg ttcgatgcgt
     2521 gctgaggtcg gctgattcat gccggtgcgc cgagcgcgac cggtgcgctc gcggtctgtt
     2581 gcaacatcgg gaaccccaac gcctcccgac tttcgtagta gctcctggcg accccaccgg
     2641 caagcttgcg cacccgcagg atgtagcgct ggcgttcggt caccgagatc gcgcgccgcg
     2701 catccagcag attgaacgtg tgcgatgcct tcaatacctg ctcgtaggct ggcaggggca
     2761 gcttcgcttc cagcagttcg ttggcggcct tctcgtgttc gtcgaatcgt tgcagcagaa
     2821 ccgcggtgtt cgcaaattcg aagttgtagc gggactgctc cacttcgttc tggtgaaaca
     2881 cgtcgccgta ggtcacacgt ccgagcgggc cgtcggtcca gacgatatcg aagatgctct
     2941 cgacgccctg caggtac
//
LOCUS       DATMRM010000239         4548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_127293, whole genome shotgun sequence.
ACCESSION   DATMRM010000239 DATMRM010000000
VERSION     DATMRM010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127293"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VNZ06_06925"
     CDS             <1..795
                     /locus_tag="VNZ06_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HWW20521.1"
                     /translation="ASDRTQGALLLAAGSVLHAPEIAVAASAGMARVRVSSQPAIVVV
                     STGNELVEPGDPIAPHQIRRSNAYAVAAALRQRGFQRVADDHLADDESQLRGRLRMHL
                     DTHEVLVLSGGVSMGKFDLVPKVLEEIGVRCVLHGIAQRPGRPMWFGTTNQGRAVFAL
                     PGNPVSTLMCLIRYVVPALYAAMGVPASSAQRIALAAPIEWTSKLTGFFPVRISCDDW
                     ARPWGTPCPTNGSGDFASLALTDGFIELPPGPADFAKGYVARLYRW"
     gene            835..1881
                     /gene="moaA"
                     /locus_tag="VNZ06_06930"
     CDS             835..1881
                     /gene="moaA"
                     /locus_tag="VNZ06_06930"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HWW20522.1"
                     /translation="MPADVQPLHPRQHLLRDSFQRPLRDLRLSVIDRCNFRCPYCMPR
                     ETYHAQYRFLKSSERLEFTELVRLTRLFLQLGVTKLRITGGEPLLRPGLTDLVGDVTT
                     LPGIEDVALTTNGVLLAQHAAALRAAGLRRVTVSLDSLDPDTFKTMAGGFAQLDDVLS
                     GIAAARCAGLSPIKINAVVQRGVNDHQILDLIERFRGTGVIVRFIEYMDVGNRNDWAR
                     ERVVPSHELLARVSQRWAVTALEPTYSGEVARRYGLTDGSAEIGFISSVTQPFCGNCS
                     RARLSSDGRFYTCLFAAEGTDLRAPMRGGASDEELLSLIRATWLHRADRYSELRENLR
                     AHGVQHVEMNYVGG"
     gene            1888..2400
                     /gene="moaC"
                     /locus_tag="VNZ06_06935"
     CDS             1888..2400
                     /gene="moaC"
                     /locus_tag="VNZ06_06935"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HWW20523.1"
                     /translation="MPPNPRAASLTHLDANSRPAMVNVAAKAVTHRIAEAEARLRLPP
                     AVARVLRQSGHRTAKGPVFDTAILAGVMAAKRTSELIPFCHPLALEQCHIQIGFASSR
                     ARGAVLQIRCRVEVHHKTGVEMEALTGASVAALTVYDMCKALSHDMRIEGVRLLSKVG
                     GKRTVRRKGK"
     gene            2397..3227
                     /locus_tag="VNZ06_06940"
     CDS             2397..3227
                     /locus_tag="VNZ06_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HWW20524.1"
                     /translation="MTDPRLFGLVLSGGHSRRMQRDKAGLEYAGRPQLARAMDLLQPL
                     VARAFVSVRADQRDERTRAGYDSIADIHAELGPLGGIHAALTTHPAHAWLVLACDLPF
                     LDAATLQHLIAHRARARLVTAYRSSSDGLPEPLCAIFEPSARAPIEHWIAQGQKCPRK
                     LLSQSDVELLDLPNAHALDNINTVEEYSVAHAALGCARRLNVRYFAMLREQAGCSFEQ
                     LQTRAVTPADLYEELRQRHGLTLKPQFLRVAVNDEFGDWNLTLADGDTVVFLPPVAGG
                     "
     gene            3232..3714
                     /locus_tag="VNZ06_06945"
     CDS             3232..3714
                     /locus_tag="VNZ06_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HWW20525.1"
                     /translation="MSETIAAEFCLSESPLLTDELRRGLQDPVCGGYVAFEGWVRNAN
                     EGRTVLRLEYEAFAPLALREGQRILARARERFGVRHVCCAHRVGSLALGELAVWVAVS
                     AVHRAEAFDACRYIIDEVKHRVPIWKKEHYQDGDSGWVNCEHCAAAPHHHEPLGVTTP
                     "
     gene            complement(3736..>4548)
                     /locus_tag="VNZ06_06950"
     CDS             complement(3736..>4548)
                     /locus_tag="VNZ06_06950"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HWW20526.1"
                     /translation="GSESWRSSAAIHACLVEGLKAAQLPPAAIQLVPTTDRAAVGEML
                     SGMQDYIDVLVPRGGKSLVARVQAEARVPVIGHLEGVCHVYVDRDADLAMARSITVNA
                     KMRRTGICGAAETLLIDRDCASNVAPTLINDLLAAGCEVRGDESIRQHDARVKPVTDA
                     DWVTEYLDAIIAVREVSGVDEAISHIGRYGSAHTDAIVTANQHTAERFLSRVDSAIVL
                     HNTSTQFADGGEFGMGAEIGISTDRFHARGPVGVEQLTSYKYVVRGSGQIRP"
BASE COUNT          775 a         1491 c         1481 g          801 t
ORIGIN      
        1 gccagcgatc ggacgcaggg tgcgttgctg ctggccgccg gcagtgtgct gcacgccccg
       61 gagattgcgg tggcggcctc cgcaggtatg gcacgggtac gcgtcagcag ccagcccgcc
      121 atcgtcgtgg tgtccaccgg caatgaactg gtcgaacccg gagacccgat cgcgccccat
      181 cagatccgcc gctccaatgc ctacgccgtg gccgcggcgc tgcgccagcg cggctttcag
      241 cgcgtggccg acgatcatct ggcggatgat gagtcgcaac tgcgcggacg cctgaggatg
      301 cacctcgaca cgcacgaggt gctggtgttg tccggtggcg tctcgatggg caaattcgat
      361 ctggtaccca aagtgctgga ggagatcgga gtgcgctgcg tgctgcacgg tatcgcgcag
      421 cgaccagggc ggccgatgtg gtttggcacc acgaatcagg ggcgggcagt ctttgcgctg
      481 ccgggtaatc cggtctccac actcatgtgt ctgatccggt atgtcgttcc ggccctgtat
      541 gccgcgatgg gtgtgccggc cagcagcgcc cagcgcatcg ctttggcggc gccgatcgag
      601 tggacgtcga aactgacggg attcttcccg gtgaggatct cctgcgacga ttgggcgcgg
      661 ccgtggggta cgccctgccc gaccaatggg tcaggcgact tcgcctccct ggccctcaca
      721 gacggtttca tcgagctgcc acccggaccc gccgacttcg ccaaaggtta tgtcgcaagg
      781 ctgtatcgct ggtagcgaac ctgcgccagt ggcctaatgt cgaatactca aggtatgcca
      841 gccgacgtcc aacctctgca tccgcgccag cacctgctgc gcgacagctt ccagcggccg
      901 ctgcgcgatc tgcggctgtc ggtgatcgat cgttgcaatt tccgctgccc gtactgcatg
      961 ccgcgcgaga cctatcacgc gcagtatcgc tttctcaaat cgagcgagcg gctcgagttc
     1021 acggagctgg tccgcctgac gcgtctgttt ctgcaactcg gcgtgacgaa gctgcgcatc
     1081 acgggcggcg aaccgctgct gcggccaggg ctgacggatc tggtcggcga tgtgacgaca
     1141 ttgccgggta tcgaagacgt ggcgctgacc accaacggcg tactgctggc gcagcacgcg
     1201 gccgcgctgc gtgcggcggg gctgcggcgg gtgacggtca gtctggatag cctggacccc
     1261 gataccttca agacgatggc cggtggtttt gcgcagctgg atgatgtgct ctcgggcatc
     1321 gcggctgccc gctgcgccgg actgtcaccc atcaagatca acgcggtcgt acagcgcggt
     1381 gtcaacgacc atcagatcct cgatctcatc gagcgcttcc gtggcaccgg cgtcatcgtc
     1441 cgcttcatcg aatatatgga tgtcggtaac cgcaacgatt gggcccgcga gcgcgtcgta
     1501 ccctcgcacg aactgctggc gcgcgtctcg cagcgctggg cagtcacggc gctggagccg
     1561 acgtattccg gcgaggtcgc gcgtcgctac ggcctgactg acggcagcgc cgagatcggc
     1621 ttcatctctt cggtgacgca accgttttgc ggcaactgct cgcgtgcgcg cctgtcttcg
     1681 gacggcaggt tctacacctg tctgttcgct gccgaaggca cggatctgcg tgcgcccatg
     1741 cgcggaggcg ccagcgatga ggagctgttg agcctgatcc gcgccacctg gctgcatcgt
     1801 gctgaccgct acagcgaatt gcgcgaaaat ctgcgggcgc atggcgtaca gcacgtcgag
     1861 atgaactacg tcggcggctg aagcaacatg ccacctaatc cgcgcgccgc atcgctgacg
     1921 catctggacg ccaactcaag accggcgatg gtcaacgtcg cggccaaggc agtgacgcat
     1981 cggatcgccg aggccgaggc gagattgcgg ctgccgccgg cggtcgcgcg tgtgttgcga
     2041 cagagcggcc atcgaaccgc gaagggaccg gtattcgaca cagcgattct cgcgggcgtc
     2101 atggccgcca agcgcacgtc cgagctgata ccgttctgcc atccgctcgc gctcgagcaa
     2161 tgccacatac agatcggctt tgcatccagc cgcgcgcgag gcgctgtctt gcagatacgc
     2221 tgccgggtcg aggtacacca caagaccggc gtggaaatgg aagccctgac cggcgccagc
     2281 gtggcggcgc taacggtgta tgacatgtgc aaggccctgt cgcacgacat gcgtatcgag
     2341 ggcgtaagat tgctgtccaa ggtcggcgga aaacgaactg ttcgcaggaa ggggaaatga
     2401 cagatcccag gctgttcggg ctggtgctct ccggcggcca cagccggcgc atgcagcgcg
     2461 acaaggcggg ccttgaatat gccggtagac cgcagctcgc gcgggccatg gacctgttgc
     2521 aaccgttggt ggcccgggcg ttcgtgtcgg tgcgcgccga tcagcgcgat gagcgcacgc
     2581 gcgccggcta cgacagcatt gccgacattc atgccgagct cggccccctc ggcggcattc
     2641 acgcggcgct cacgacgcac ccggcgcatg cctggctggt gctcgcgtgc gatctgccgt
     2701 tcctcgatgc cgccacgctg cagcatctga tcgcgcaccg tgcccgtgcc cgcctggtca
     2761 ccgcgtaccg cagcagctcg gatggattgc ccgagccgct atgcgcgatc ttcgagccct
     2821 cggcgcgcgc gccgatcgag cactggatcg cccagggtca gaagtgtccg cgcaagttgc
     2881 tgtctcagag cgatgtcgaa ctgctggatc tgccgaacgc gcacgcactc gataacatca
     2941 acacggtcga agagtattcg gtcgcacatg cggcgctggg ctgcgctcgc cgcctgaacg
     3001 tgcgctattt cgcgatgctg cgcgagcagg cgggctgcag cttcgaacag ctgcagaccc
     3061 gggccgtcac acctgccgat ctgtacgagg aattgcgcca gcgccatggg ctgacgctga
     3121 aaccgcagtt cctgcgcgtt gccgtcaacg acgagttcgg cgactggaac ctcaccctcg
     3181 ccgatggcga cacggtggtc tttctgccgc cggtcgcggg aggctgagcc ggtgtccgaa
     3241 accatcgctg ccgaattttg cctgagcgag agcccgttgc tgaccgacga gctgcgtcgc
     3301 gggctgcagg atccggtctg cggcggttat gtggcctttg aaggttgggt acgcaacgcc
     3361 aatgaaggcc gcacggtgct gcgcctggaa tacgaagcgt tcgcgccgct cgcgctgcgc
     3421 gaaggccagc gcatcctggc ccgcgcgcgc gaacgcttcg gcgtcaggca cgtgtgttgc
     3481 gcccaccgcg ttggatcact tgcgctcggt gagctagcgg tctgggtcgc agtcagcgcg
     3541 gtacatcgcg ctgaggcgtt cgatgcctgc cgatacatca tcgatgaagt caaacatcgc
     3601 gtgccgatct ggaagaagga gcactaccag gacggcgatt cgggttgggt gaactgcgaa
     3661 cactgcgcgg ccgcgccgca tcatcacgaa ccgctgggcg tgaccacccc ctaggctgac
     3721 ccgaacgcgt cagcgtcagg gtcggatctg accgctgccc cgcacgacgt atttgtagct
     3781 ggtcagctgc tcgacaccca ccggcccgcg cgcgtgaaaa cgatcggtcg agatgccgat
     3841 ctccgcgccc atgccgaact cgccgccgtc ggcaaactgc gtggaagtgt tatgcagcac
     3901 gatcgcactg tccacacgcg acagaaagcg ctccgcggtg tgctggttgg cggtcacgat
     3961 ggcgtcggtg tgagccgagc cgtaacgccc gatgtgcgag atcgcctcat ccaccccgct
     4021 gacctcgcgc acggcaatga tcgcgtccag atattccgtg acccaatccg cgtcagtgac
     4081 cggcttgacg cgcgcatcat gctggcgaat cgactcatcg ccccgcacct cgcagccggc
     4141 agccaacaga tcgttgatca gcgtcggcgc cacattgctg gcgcagtcgc gatcgatcag
     4201 caatgtctcg gcggcaccgc agataccggt gcgacgcatc ttggcattca cggtgatgct
     4261 gcgtgccatc gccagatccg catcgcgatc gacgtacacg tgacaaacgc cttcgaggtg
     4321 cccaatgacg ggcacccgcg cttcggcctg tacgcgcgca accagactct tgccaccgcg
     4381 cggtaccagc acatcgatgt agtcctgcat gccggacaac atctcgccga ccgccgcgcg
     4441 gtccgtggtc ggaaccagct ggatcgcggc cggcggcaac tgcgcggcct tcagaccttc
     4501 gaccagacag gcatgaatcg cagcgctcga gcgccagctc tcggagcc
//
LOCUS       DATMRM010000240         8081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1380482, whole genome shotgun sequence.
ACCESSION   DATMRM010000240 DATMRM010000000
VERSION     DATMRM010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380482"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2714)
                     /locus_tag="VNZ06_06955"
     CDS             complement(<1..2714)
                     /locus_tag="VNZ06_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWW20527.1"
                     /translation="MSVDSSYQSDAINKRRQLRELMSARRATELRPIGASSSTLIPDL
                     QNRHEPFPLTDIQEAYWLGRDTAFPLGGVGIHGYFELLCPDLDVERLQEAWNAVVRRH
                     DMMRAIVLCDGSARIIEHVPCYIIPVEDLRDRTTQEQQRHRHELRARMSHQVFDVEKW
                     PLFELRIVRLDQHRFRLHYSEDAIHSDLRSTALVMSDWLQLYRDPAVQLRPIPLSYRD
                     YALAMHRRPQSADYARAKRYWDARLDTLPESPQLPLKGNLTGLRMAFARRKFTLTASR
                     WQRFREQASKHGLTPSDALLATYVDVLGLWSTRRRFALNVTLQNRLPLHSQVNEISGD
                     FTSFVLLDVDAGSSGGFVERASRYRDQLWSDLEHNEMSGVSVLRELARRRRSGDVVAP
                     YVFTSALGTSGYRAIEELGEVEFEITQTPQVLLDQQAVEWRGQLVLTWDCVDAAFADG
                     VIDEMFSWMSQRINQLCDEASEWSAQSRVSIPVAQQRRRDAFNATSAEFPRERLERMF
                     ARRAQEDRGRVAIVDHRGSMSYGELDDASSGLALALRAVPDLDAAVPVAIGMRKSRDE
                     IVAVLGILKAGLSYLPLEISQPASRIERILSTAGCRAVIVDDDAPEWISRLEGRRRVL
                     SLKDGQSAGNFAELPEIRDSDVAAYVLFTSGSTGDPKGVVVTHDSVVNRMLDVVERFA
                     IGRHDRAMGLTGLHHDLAVFDIFGMLSVAGGTLIVPSPDVIEAPAKWPDFIDAHGVSI
                     WNSVPAYLDLLLAHCEARPAQAARICGLRLALIAGDFIPPSLPLRFRGNTHGRFIALG
                     GPTETTVWDICYPVEALPHGWSAIPYGKPMRNAKYFVLREDLSECPDWVEGELCIAGA
                     GLAIGYLRDTVLSGARFVTHPESGERIYRSGDRGRVRPDGLVEILGRL"
     gene            complement(2711..8032)
                     /locus_tag="VNZ06_06960"
     CDS             complement(2711..8032)
                     /locus_tag="VNZ06_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HWW20528.1"
                     /translation="MHDEPTTDIAIIGVAGRFPSAPNIERYWQLICQGVEGIHALTAN
                     EVARAGVSETVLRQPNYVLRGGCLDSIEELDAGFFSLSPREAELIEPQQRLMLELAWE
                     SLESAGIDVRRMPDVAVFAACSKPGYLAAGTTPFDPSWLGSTAHFQTAIATDRDYLAT
                     RVSYKLGLSGPSVTVQTACSSSLVAVHMACQSLLLGECEVALAGGVALQVPHRSGYLW
                     QEGGYSSRDGRCRPFDAAADGTVFGSGGGVVVLRRLSEALAAADSIIAVIKGTAIGND
                     GSQKIGFTAPSIAGQARVIRAAQAAAGVAAQQIGYVEAHGTGTPLGDPIEVAALTQAF
                     GQSGAATGRCAIGSVKGNIGHLDTAAGVAGLIKTALALKQGVVPPSIHYERANPAIDF
                     ASGPFYVNTRLRPWSAELPYAGVSSFGIGGTNAHAVLGPAPSSQQCSGPRAGPQLLVI
                     SARERAALQALAREYEQWLNGASAGSCEASLIAAAAAMRRTHHEYRLAVVGDTAATLA
                     QSLRRSSQQPPLSEAAGSTARSSADAVWVFSGQGGQWIGMGRELLQAGGAFSRTVEQI
                     DARLQQLEGWSVRAVLEGASGARPLSEVQVSQVAVFTLQMGLAAAWQELGLRPAAVVG
                     HSMGEVAAAYVAGALTLQEALEVICARARMLDQHGPRGLMALVDLPADQAEQLLREQP
                     EGSVWIAAYNSPRNTVLAGDSHSMRTLLLRLRGSQIYCREFETAGVAGHGPGMQAAAR
                     ALEEQLSALPARAVSVPFFGTVNGACEQTLGAGYWSENLCKPVRFAQAIQALKAGGHA
                     VFLELGPRPALAAAILETESNDALKVLASCDRPNARARFLQSLGELYTRGFNPHWQHI
                     HGTHVPNIALPTYSWQKKKYWMDSLKQPLKPNHAAATSVSAIGRRIYSPALADALFET
                     EVSGNCALFRSHEIFGRTTVPFAAVAEMALQALNAYAPDLAFSLLSLQHETPLQLEGD
                     QSVKLQTLVRLKGRRPAHIEIFAQDRSCDSDWRRIGIAQCEVCAERMAPVALANLRLR
                     LRDQIPTERLWSDAASEGIALREQYRSVEQAYGNGNEGLVRLRSSPLGTEGIRHPSML
                     DGCLQAAQLLPLTGSAGNPTVPVSADEINVAWGVPIAWSWAHLDSQSLVGTTKVDFDF
                     YGSVGEYLGYAKGVEFRTVQAFASTVEVASGFQVCWEKVPILSPPSATGAGNWLVVPD
                     QRLVWKRFASLVTAAGGSCTTYPCQSSRAELSGVVDFRALDAVQIDGADELCCQAAQF
                     LQEMIAERTQVSPPLYIVTAGAQAVEPADDIFCAQAALWGLTRSVAHEHPELNLRLVD
                     LDSRDLPGSVRSFIERRDQVDQLLQVAVRNGVIRAARLRPLKAHSTKHSMLRGDGTYL
                     VMGGLGGLGIRLASWLLEKGAGVLVLAGRSVSDEAARAAAALSRADRTVHVRKVDCGS
                     SEQVSELICEIGRSMPPLRGVIHTALTLRDAVFVNLTRESFKEVFRPKVHGAWLLHEH
                     TRHLPLDFFALFSSVAAVLGAGGQANYGAACAAMDAVAHHRRALGLSAITINWGPWDY
                     PGSPSRGPFVKDSARYQALTVDQGFGWLDRLLAGSAAEVGPTQALVMRLRRAAWRSTG
                     PSLLQPLLREDIADELAQRRADAGAAVSLRMGSMSRDQRIAALIGYLTERLARRLRCA
                     VISVPIEQSLPSMGLESLTIIELRNEIGSDLGAYISPRSLIAARSIRAIAALIADSLE
                     SDRGTAISLSRETALDVDSMSDEEVAAALGRILAERTVNGISVGANL"
BASE COUNT         1635 a         2698 c         2284 g         1464 t
ORIGIN      
        1 tccaaccgac ccaggatttc gaccaggccg tcaggtcgaa cccgacccct gtcgccggag
       61 cggtagatac gctcgccgct ttcaggatga gtcacgaagc gtgcgcctga cagaacagta
      121 tcgcggagat aaccgatagc cagaccggcg ccagcaatac atagttcgcc ttccacccag
      181 tccgggcact cgctgaggtc ctcgcgcaag acaaagtatt tcgcattgcg catcggcttg
      241 ccgtacggga tggcggacca accgtgcggc aatgcttcga ccgggtagca aatgtcccag
      301 accgtcgttt cggtcgggcc acccagcgca atgaagcgac catgggtgtt gccgcggaag
      361 cgtagcggca gcgaaggcgg gatgaagtcg ccggcgatga gcgccagtct cagcccgcag
      421 atgcgcgctg cctgagctgg gcgcgcttca cagtgggcca acaggagatc cagatacgcc
      481 ggcacagaat tccagatgct gacgccgtgc gcgtcgatga aatccggcca cttggcagga
      541 gcttcaatta catccggcga tggaacaata agtgtgccac ccgccactga caacattccg
      601 aaaatatcga acaccgcgag gtcatgatgt agaccggtga gccccatcgc gcggtcatgg
      661 cgaccgatag cgaatcgttc gacgacatcc agcattcgat tcaccacgct atcgtgagtg
      721 acgacgactc ccttcgggtc accggttgat cctgaggtaa acaagacata cgcggcgaca
      781 tccgagtccc gaatctccgg caactcagca aagttcccgg cagattgacc atccttcagg
      841 gacagaaccc gccgacggcc ctcaagccga gatatccatt caggcgcatc atcatcgacg
      901 atgaccgcgc gacagccggc ggtggacaga atgcgctcga tacgggacgc tggctgggat
      961 atttcgagtg gtagatagga cagtcccgcc ttcagaatcc ccaataccgc cacaatctca
     1021 tcgcgactct tgcgcatgcc gatcgccacc ggcaccgctg catccagatc cggaacggcg
     1081 cgcagcgcca gagcaagccc tgaactggca tcgtcgagct cgccgtagga catcgagccc
     1141 ctatgatcga cgatcgcaac gcggccgcgg tcttcctgcg ccctcctggc aaacattctc
     1201 tcgaggcgct cgcgcgggaa ttcggccgag gtggcattga atgcgtcacg acggcgctgc
     1261 tgcgcaacgg ggattgaaac gcgggactgt gcgctccact cactcgcctc gtcgcaaagt
     1321 tgattgatgc gctgactcat ccatgagaac atctcatcga tcacaccatc ggcaaacgcc
     1381 gcatcgacgc agtcccaggt cagcaccagc tgaccgcgcc actcaacagc ctgttgatcc
     1441 agcagtacct ggggcgtctg ggtgatctcg aattcaactt cacccaattc ctcgatcgcc
     1501 cgatacccac tggtgccaag tgcgctggtg aagacgtacg gtgccactac gtcgccggat
     1561 ctgcggcgtc gagccaattc tcgcagtacg ctgacgccgc tcatctcatt gtgttcaagg
     1621 tcactccaca gctggtcacg atagcgcgac gcgcgttcga cgaagccgcc gctcgatccg
     1681 gcgtcaacgt ccagcagtac gaaagaggtg aagtcaccac tgatctcgtt gacctgcgaa
     1741 tgcagcggca atcggttttg aagtgtgaca ttcaacgcaa accggcgcct ggtgctccac
     1801 agccccagaa cgtccacgta ggttgccagt agtgcgtccg atggtgtcag cccatgcttc
     1861 gatgcctgtt ctctgaatcg ctgccaacgc gacgcagtca atgtgaactt tcgtcgcgcg
     1921 aaagccatgc gaaggccagt caggtttccc tttagcggaa gctgaggaga ctccggaagc
     1981 gtatccaacc gggcatccca ataacgcttg gcgcgcgcgt agtccgcact ttgcggacgc
     2041 cggtgcattg cgagtgcgta gtcccgatag gagagcggta tcggcctcaa ctgaacagcg
     2101 gggtcgcgat agagctgtaa ccagtctgac attacaagtg cggtactgcg cagatccgaa
     2161 tggatggcgt cctcgctgta gtgcaagcga aagcgatgtt ggtcgaggcg cacgatgcgc
     2221 agctcgaaca gaggccactt ctcgacgtca aagacctgat gcgacatgcg cgcgcgcagc
     2281 tcatgacgat gtcgctgctg ttcctgcgtc gttcggtcgc gcaggtcctc aaccggaatg
     2341 atgtagcacg gaacgtgttc gatgatgcgc gccgatccgt cacaaagcac gatcgcccgc
     2401 atcatgtcgt gccttcgaac aactgcgttc caggcctcct gcagccgctc gacatcgagg
     2461 tccggacaca gcagctcgaa atagccgtgg atgccgacac ctcccaatgg gaacgcggta
     2521 tctcggccga gccagtacgc ctcctgaata tcagtcagcg ggaacggctc atggcggttc
     2581 tgcaaatcgg gaatcagcgt ggaggatgat gcaccgattg gcctcagctc ggtggcgcgc
     2641 cgcgcactca tcaactcacg caactggcgg cgcttgttga tggcgtcgct ctgataggaa
     2701 ctgtcgacgc tcaaagattt gctcctaccg agatgccgtt caccgtgcgt tccgccaata
     2761 tcctgcccag cgcggcggcc acttcctcgt ccgacatcga atcgacgtcc agtgctgttt
     2821 cacgactgag actgatggcc gtgccccggt cggactccag actgtccgcg atcagcgccg
     2881 caatagcgcg aatgctgcga gccgcgatga gcgagcgggg agaaatatat gcgcctaaat
     2941 ccgatccgat ctcgtttcgc agttcgatga ttgtcagtga ctcaagcccc atactgggca
     3001 gtgattgctc aatgggtacg gagatcacag cgcatcgcag acgtctcgcc agccgctcag
     3061 tgagatagcc gatgagtgct gcgatgcgtt ggtcacgcga catcgagccc atgcgcaagc
     3121 tcacggcagc gccagcatcg gcccgtcgtt gcgccagttc gtccgcgatg tcttcacgca
     3181 agagtggttg caggagactg gggccggtac ttcgccacgc ggcacgacga agtctcatga
     3241 ccagcgcctg cgtcgggccg acctcagcgg cgctgcccgc cagcaggcga tcgagccagc
     3301 caaagccctg gtcaactgtc agcgcctgat atctggcaga atccttgaca aagggccctc
     3361 gcgacggcga gcccggataa tcccaaggcc cccagtttat cgtgatggcg ctgagcccca
     3421 aggcgcgtcg atggtgtgcg acagcatcca tcgctgcgca tgccgcgccg taattcgcct
     3481 gcccgcccgc gccgaggaca gcggcgactg atgagaatag agcgaagaag tccaggggca
     3541 ggtgccgcgt gtgctcatgt agaagccatg cgccatgcac tttgggtcga aatacctcct
     3601 tgaaggactc tctcgtcaga ttgacgaaca ccgcatcgcg tagcgtcagc gccgtgtgga
     3661 tgactcctcg cagcggaggc attgaacggc ctatctcgca gatcagctcg ctgacctgtt
     3721 ccgaactccc gcaatccacc ttgcggacat gcaccgttcg gtcagcgcgc gacagcgccg
     3781 ctgccgcacg agcggcctcg tcggacaccg agcgcccagc cagcaccagg acaccagcac
     3841 ccttctccag cagccaactc gccaggcgga ttccaagccc gcccaaacct cccatcacaa
     3901 gataggtacc gtcccctctg agcatcgagt gcttggtgga gtgtgctttg aggggtcgca
     3961 gccgcgctgc ccgaatgact ccgttacgga ccgccacctg cagcagctgg tcaacctgat
     4021 ctcgacgctc aataaaagag cgcacactgc ctggcaggtc cctcgaatcc aggtctacga
     4081 gacgtaggtt cagttccgga tgctcgtgcg ccacgcttcg tgtcagaccc cacaaagccg
     4141 cctgggcaca gaatatgtcg tcggccggct caactgcttg agcacctgcg gtcacaatgt
     4201 agagcggtgg ggagacttgc gtcctctctg cgatcatttc ctgaagaaac tgtgccgcct
     4261 ggcaacacag ctcgtctgcc ccgtcgatct gcacggcgtc cagcgcccga aagtcaacga
     4321 ctccgctcag ttcagccctg ctggactggc aggggtaagt cgtgcacgag ccgccggcgg
     4381 cggtgacgag agacgcgaac cgcttccaga ccagccgctg atcgggaact accagccagt
     4441 tgcccgcgcc cgtcgcgctc ggcggcgaca ggatcggcac tttctcccaa caaacctgaa
     4501 agccggatgc aacttcaacg gtgcttgcga aggcttgcac tgttcgaaat tccacacctt
     4561 tcgcatagcc caggtattca cccacagagc cgtagaaatc aaagtccacc ttggtcgtgc
     4621 cgacgaggct ctgcgagtcc agatgcgccc agctccaggc gattggcacg ccccacgcaa
     4681 cgttgatttc atcagctgat acaggaacgg tcggattccc ggcgctgcct gtcagcggca
     4741 ggagttgcgc cgcctgcaag cagccgtcca gcatgctcgg atggcggatc ccctcggtgc
     4801 caagtggcga tgatcgcagg cgcaccagtc cctcgttacc attgccgtag gcctgttcga
     4861 cgctgcggta ctgttcgcgc agcgcaatac cttcgctggc ggcatcgctc cataggcgct
     4921 cggttgggat ttgatcgcgc agacgcaacc tcaggttcgc cagtgcgaca ggcgccatgc
     4981 gctcggcgca gacctcgcat tgagcaatgc caatccggcg ccaatcggaa tcgcaactac
     5041 gatcctgagc gaatatctcg atgtgggcag gccgtcgccc tttgagtcgc acgagcgtct
     5101 gcagttttac cgattggtca ccctcgagct gcagaggtgt ctcatgctgc agggaaagca
     5161 gactgaaagc gaggtcgggg gcataggcat tgagcgcctg aagagccatt tcggcaaccg
     5221 ccgcaaaggg cacggtcgtg cgaccgaaga tctcgtgact acgaaacagc gcgcagttgc
     5281 cactaacctc ggtctcaaag agtgcgtctg cgagcgcagg ggaataaatt cggcgcccga
     5341 ttgcgctgac gcttgtcgcg gcagcatggt tgggcttcag aggctgcttc aggctgtcca
     5401 tccagtattt tttcttctgc caggaatagg tgggcagggc gatgttgggg acgtgcgttc
     5461 cgtgaatgtg ctgccagtgc ggattgaatc cccgggtgta gagctcgccg agggattgca
     5521 gaaagcgggc gcgggcattg ggacggtcgc acgaagcgag caccttcagc gcatcgttcg
     5581 actcggtctc caggatcgct gcggcaagcg caggtcgcgg accgagctcc aggaacactg
     5641 cgtgaccgcc agccttcagt gcctggatgg cctgcgcaaa gcgaaccggc ttgcacaggt
     5701 tctcgctcca gtagccagca cccaatgtct gctcgcacgc tccattcacc gttccaaaaa
     5761 aaggtacgct cactgctctg gctggcagcg ccgagagctg ctcttcaagc gctcgggcag
     5821 ccgcctgcat gccgggaccg tgaccggcca cgcccgctgt ctcgaactcc cggcagtaga
     5881 tctgagatcc tctgagtctt agcaacagag ttctcatgga gtgtgagtca ccggccagca
     5941 cggtgttgcg cgggctgttg taagcggcga tccacaccga gccctcaggc tgttcgcgca
     6001 gcaactgctc ggcctgatcg gccggcagat ccaccagcgc catcagaccc cgtgggccat
     6061 gctggtccaa catgcgtgca cgcgcacaga tcacctctag cgcctcttgc agtgtcaggg
     6121 ctcctgccac atacgctgcc gccacctccc ccatgctgtg cccgactacc gcagccggac
     6181 gcaaccccaa ctcctgccac gccgcagcca atcccatctg cagcgtaaag accgccacct
     6241 gcgatacctg aacctcactg agcgggcggg cgcccgaagc cccttccagc accgcacgca
     6301 ccgaccagcc ttcaagctgc tgcagccgtg catcgatctg ctcaacggta cgactaaaag
     6361 caccccccgc ctgcaacagc tcgcgcccca tccctatcca ttgaccgccc tgccctgaaa
     6421 atacccacac cgcatccgca ctgctgcggg ctgtgctgcc cgccgcctca ctgagcggcg
     6481 gctgttgact gctccggcgc aggctctggg ccaaggtagc cgccgtatca cccaccaccg
     6541 ccaggcgata ctcatgatgc gtgcgacgca tagccgccgc cgccgcaatc agcgatgcct
     6601 cgcagctgcc tgccgatgca ccgttcagcc actgctcata ctcacgcgcc agcgcctgca
     6661 gcgccgcgcg ctcacgcgcc gatatcacca gcagttgcgg cccagctctc gggccgctgc
     6721 actgctgact gctcggagca ggacccaaca ccgcatgcgc attggtcccc ccgatcccaa
     6781 aggagctcac tcccgcatac ggcagctctg cgctccacgg ccgcagccgt gtgttcacgt
     6841 aaaacgggcc cgaagcaaaa tcaatcgctg gattggcccg ctcataatga atgctcggcg
     6901 gcactacacc ttgcttcaat gccagtgccg tcttgatcag acctgccact cccgccgcag
     6961 tatccaaatg accaatgttg cccttcactg atccgatcgc acaccgcccc gttgctgccc
     7021 ctgactgccc aaaggcctgt gtcagcgccg ctacctcaat cggatctccc agcggagtgc
     7081 cggtgccgtg tgcttccacg taaccaatct gctgcgccgc tacacccgca gcggcctggg
     7141 ccgcacgaat cactcgggcc tgcccggcaa tactgggcgc cgtaaaacct atcttctgcg
     7201 atccatcatt accgatcgcc gtgcccttga tgaccgctat gatcgagtca gctgctgcca
     7261 gcgcctcact cagccgcctc agcaccacca ccccaccgcc gctcccgaac accgtcccgt
     7321 ccgccgcagc atcaaacggt cggcagcgtc catcccgcga ggagtagccc ccttcctgcc
     7381 ataaatatcc gctccgatgc ggcacctgca gcgctacccc tccagccagc gctacctcgc
     7441 actcccccag cagcaggctc tgacatgcca tgtgcaccgc taccaacgag ctcgaacacg
     7501 ccgtctgcac cgtcacactc ggaccactca accccagctt gtacgatact cgagtcgcta
     7561 aataatcccg gtccgttgca atagcggttt gaaagtgcgc agttgagccg agccaactcg
     7621 ggtcgaatgg agtggtgccc gccgccaggt atcctggctt gctgcaagcg gcaaacacag
     7681 ccacgtcagg catgcgacgc acatcgattc cagcactttc gagtgattcc caggcaagtt
     7741 ccagcatcag gcgttgttgc ggctctatca gttccgcttc ccgcgggctc agcgagaaga
     7801 agccggcgtc cagttcctct attgagtcaa ggcatcctcc gcgcaacaca taatttggct
     7861 gtctcagcac agtctcggag acgcccgccc gagcgacctc gtttgcagtc aaggcatgta
     7921 tgccctcaac tccctgacat atcagctgcc aatagcgctc gatgttgggc gccgaaggaa
     7981 agcgtcctgc cacgccgatg atggcgatat ccgtggttgg ctcgtcgtgc acggctactc
     8041 cgaccgccgc tcgcgagcca gggcgagggc acgcctgcgc a
//
LOCUS       DATMRM010000241         7068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4169246, whole genome shotgun sequence.
ACCESSION   DATMRM010000241 DATMRM010000000
VERSION     DATMRM010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4169246"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /gene="rfaE1"
                     /locus_tag="VNZ06_06965"
     CDS             complement(<1..1042)
                     /gene="rfaE1"
                     /locus_tag="VNZ06_06965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HWW20529.1"
                     /translation="MSHTVLLDLLSKFHSARVLVLGDVMLDRFVYGSVERISPEAPIP
                     VMRVERTVDMLGGAANVARNVATLGGTALLVGVIGSDATAEAMRAQLHLIPTIQARLI
                     SDASRPTTAKTRHVADRQQILRTDVESKAPLNDATAAEVLVQFRAQLGVADVVIISDY
                     AKGVLTEALTAAAIGAAREAGKLVLVDPKSRSFAKYRGASVLTPNRHELEVACGHECS
                     SDEQVVAGARALLGPAQCDAMVVTRGKAGMSIVRRDGSAEHIRTVAKEVYDVSGAGDT
                     AVATLGLSLAHGADIAHAAQLANAAAGVVVGKYGTASVTAGEVTARLGLAEEGPMSRK
                     HFSLDSVQQQVAR"
     gene            complement(1053..1658)
                     /locus_tag="VNZ06_06970"
     CDS             complement(1053..1658)
                     /locus_tag="VNZ06_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HWW20530.1"
                     /translation="MEAKVATGFDIEAFLHREAGEHRDAFESLLRTVKTPFSQTLSIW
                     ESAIRQGGKLLFFGNGGSASDSQHLAAECVIRYHTDRPAIAAIALTTDSSVLTAGSND
                     MGFEQVFARQIEALGRPGDVAVGISTSGRSRNVLNGLREARARKLHSVGFTGGNGGDM
                     PGLCDVSIVVPSSITARIQEMHIFVGHLLCKALEQRLGLTL"
     gene            1737..2669
                     /gene="hemF"
                     /locus_tag="VNZ06_06975"
     CDS             1737..2669
                     /gene="hemF"
                     /locus_tag="VNZ06_06975"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018927302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HWW20531.1"
                     /translation="MTREEQIHGYLSSLQQNLCQSFGQLDGGKFVPDPWTREPSATGL
                     TGCGISAVLEGGEILERAGVGFSDVGGASLPPSATARNPQLAGRRYRAMGVSVVMHPR
                     NPHVPTSHMNVRFLSTGDTWWFGGGFDLTPYLVNEQDVIAWHRAAAAACEAFHPSLYD
                     HLRKSCDEYFFVRHRGEARGIGGIFYDDLNEATPQFGMSWERCFEFMQAVAGRYCETY
                     LQIVRARYQLPYAARERDFQLYRRGRYAEFNLVLDRGTLFGLQSGGRAESILMSMPPL
                     VRWAYRSPDAELDARLLPYLTTLRGHDWRARAEV"
     gene            2738..3808
                     /locus_tag="VNZ06_06980"
     CDS             2738..3808
                     /locus_tag="VNZ06_06980"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HWW20532.1"
                     /translation="MFTTQRPTAAGAVTAGILFTALTCLSAGYGADNLAAPTAPAAPA
                     AIAPATPTEAAAQLPPPQSAELTEIVIHSTEPRFVMPTRRDRIGRIWAPVYINGKGPF
                     RLVLDSGANHSGITEQVAVILGLPSDSSQQVMLRGVTGSAAVPTVRVNSFVVGDVQEG
                     AERLPIVTDALGGAEGVLGTDGMQNRRIYIDFRHDLISITRSRSEHADAGYRVIPFET
                     MRGNLVVVDATVGGIHTKAIIDTGGQVTIANTALRQALERRKSQLLGRAIHIEGVTTD
                     IQDADEINTPPLRIGGVDEGVASVIEIRFRDISFGDMRIFEHWRLTDEPAMLVGMDAL
                     GLLDTLIIDYRRHELQIRMRPQ"
     gene            3876..5513
                     /locus_tag="VNZ06_06985"
     CDS             3876..5513
                     /locus_tag="VNZ06_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HWW20533.1"
                     /translation="MTQFNWSNPYPSTRVPVFARNVVCTSHPLAAQVGLKMLWKGGNA
                     VDAAIAAAATLTVVEPVSCGLGGDAFALVWDGTQLHGLNASGYAPAAWSVEYFQRKYG
                     RDPGMSQRPMRGWDTVTVPGVIAGWQALHQRFGTLPLADILEPASEIAERGYAVSPIV
                     AQKWEAAVPELRTQPGFAATFMPAGRAPKVSELIRLPDHARTLRMLGLKGARDFYEGD
                     IAEKIAQFAKSEGAAMTLDDLRSFRAEWVEPISRTYRGYTVHEIPPNGQGIAALMALG
                     ILSQFDLKALAVDSVESQHLQIEAMKLAFTDLYHYVADPRSMQVTPAQLLDDAYLASR
                     AKLIHRSRAGEYHCGMPAGGGTIYMSAADQNGMMISFIQSNYMGFGSGVVVPGTGISL
                     QNRGYGFSMNPRSPNAVAARKRPFHTIIPAFLTRDVGVQRQAVMSFGVMGGDMQPQGH
                     LQTLVRMLDYGQQPQAACDAPRWKLNPDFTIDVEAQLDARTLAGLQSYGHTVRSYTDS
                     YMDFGSGQFIWRLDPQDVNRGYVAASDSRRDGLAAGI"
     gene            5573..>7068
                     /locus_tag="VNZ06_06990"
     CDS             5573..>7068
                     /locus_tag="VNZ06_06990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWW20534.1"
                     /translation="MDDEPSDMPPVEMPATVTTRRRGGARAADAEPVARVGDVLLGRF
                     RLQRIIGRGGMGVVFAANDLQMQGEPLIAIKLLNEDMRDFPVAAMALERECRKVRMLA
                     HDSIVRVFEFYRTGEQTFITMELLEGESLDAVIKRFPQGVAWNDAWMIIRAAADALAY
                     AHRQKPPFVHSDFKPSNVFCTRSGQIKVLDFGVARAARTSDKAATNLSIFDPAQYGAL
                     TPAYASAEMLAGLNADVRDDVYALACVTYEVLCGEHPFARRPADQARAMMLKPKPIAS
                     LGGRVNRALAHGLAVDRMHRTPTAAQFIEELGDPPEPPEVQRRRYLKWARAGGAIAIA
                     VIAGFGLWRLKPLIHWPSRTPSLATSSAPSPSPAPLAAPVSASRPVAASKRATPEPAE
                     IRSPASPGAVPGDRLLALLQLLGIPNDVKPTTTYPESAARKLIETSPRKVVLGSTPEQ
                     MQSALTLCRRYSSECRASWYADEKQRTATLEPFELDPTPVSVLEFRRFA"
BASE COUNT         1259 a         2308 c         2230 g         1271 t
ORIGIN      
        1 agcgagcgac ctgctgctgc acgctgtcca ggctgaaatg cttgcggctc atcggtcctt
       61 cttcggccag tcccaggcgc gcggtcactt cgccggccgt cacactggcc gtgccgtatt
      121 tgccgactac cacgccagcg gccgcgttgg ccagctgtgc cgcgtgcgcg atgtccgcac
      181 cgtgagccag gctcagcccc agggtcgcga ccgccgtgtc gccggcgccg gacacgtcat
      241 agacctcttt ggcgaccgtc cggatgtgct cggctgagcc gtcgcgacgc acgatcgaca
      301 tgcctgcctt accgcgcgtc accaccatcg cgtcacactg cgccggaccg agcagcgctc
      361 gcgcaccggc caccacctgt tcgtcactcg agcactcatg gccgcacgcg acttccagtt
      421 cgtgcctgtt gggcgtcagg actgaggcac cgcggtactt cgcaaagctg cgcgacttgg
      481 gatccaccag tacgagcttg ccggcctcgc gcgcagcgcc gatcgccgct gcggtcagcg
      541 cctcggtcag cacccccttg gcgtagtcag aaatgatgac gacatccgcc acgcccagct
      601 gcgcgcgaaa ctgcaccagc acttcggcgg cagtggcatc gttcagtggc gctttgctct
      661 cgacatcagt gcgcagaatc tgctgccggt cggcgacatg gcgcgtcttg gcggtggtcg
      721 gacgcgaggc atcgctgatc agccgggcct gaatggttgg aatgagatgc agctgtgcgc
      781 gcatcgcctc ggcggtcgca tcactgccga tcacccccac cagcagcgcg gtcccgccta
      841 acgtcgcgac gttgcgcgcg acgttggccg ccccgcccag catatcgacg gtgcgctcga
      901 cacgcatcac cggaatcgga gcctcggggg agatgcgctc gaccgagccg tagacgaacc
      961 gatcgagcat cacatcgccg agcaccagca cccgcgcgct atgaaatttg cttagcagat
     1021 ccagcagcac tgtgtggctc atgggtgccg ctctagagcg tcagacccag acgctgctcg
     1081 agcgccttgc acagcagatg accgacgaaa atatgcatct cctgaatgcg ggcggtaatc
     1141 gatgaaggca cgacaatgct aacgtcgcac aaccccggca tgtcgccgcc gttgccgccg
     1201 gtgaatccga cgctatgcag cttgcgggcg cgcgcctcgc gcaggccgtt gagcacattg
     1261 cggctgcgac cggaagtgga aatcccgacc gcaacgtcac ccggccttcc cagcgcttcg
     1321 atctgtcgcg cgaacacctg ttcgaacccc atgtcattgg atccggccgt cagcaccgag
     1381 ctgtccgtgg tcagcgcaat ggcggcgatc gccggccgat cggtgtgata gcggatgacg
     1441 cattcggccg ccaggtgctg cgaatcactg gcgctgccgc cgttgccgaa gaacagcagc
     1501 ttgcccccct gacggatagc cgactcccag attgagaggg tctgcgaaaa cggcgtcttc
     1561 accgtcctca gcagggactc aaaagcgtcg cgatgctcgc cggcctcacg gtgaagaaaa
     1621 gcctcgatgt cgaatccggt ggcgactttg gcttccatgc tgcaaatgct accgcgattc
     1681 agggcgttgc gggatgctgt gctaagctgg gcggtcaatt ccgcctcctg tccgccatga
     1741 cgcgcgaaga gcagatccac ggttacttga gttcattgca acagaacctg tgccagtcgt
     1801 tcggccagct ggacggcggc aagttcgtac ccgatccgtg gacgcgtgag ccctcggcca
     1861 ccgggctgac gggttgcggg atcagcgcag tcctggaggg cggggaaata ctggagcgcg
     1921 ccggcgtcgg cttttcggac gtcggtggcg caagcctgcc gccgtcggcg accgcgcgca
     1981 atccgcagct cgcaggacgc cgctaccgcg cgatgggtgt ctcggttgtg atgcacccgc
     2041 gcaatccaca cgtccccacc agccacatga atgtgcgctt tttgagtact ggcgacacct
     2101 ggtggtttgg tggaggtttc gacctgactc cctacctggt gaacgaacag gatgtgatcg
     2161 cctggcatcg ggcagcggcc gcggcgtgcg aggccttcca cccctcactg tacgatcatt
     2221 tgcgcaagag ctgcgatgag tacttcttcg tgcgccatcg aggcgaggcc cgtggtatcg
     2281 gcggcatttt ctatgacgac ctcaacgagg ccacaccgca gttcggcatg agctgggagc
     2341 gctgcttcga attcatgcag gctgtggccg gtcgctattg cgaaacctac ctgcagattg
     2401 tgcgggcacg ttatcagctt ccctacgctg ctcgagaacg ggatttccag ctttatcgtc
     2461 ggggacgtta cgctgaattc aaccttgttt tggatagggg aacgctgttt ggtctacaat
     2521 cgggcggtcg ggcggaatcg atcctgatgt cgatgcctcc gctcgttcgc tgggcctacc
     2581 gctccccgga tgcggaactt gatgcccgtt tgttgcccta tctgacgaca cttcggggac
     2641 acgactggcg cgcacgagcc gaggtgtgac ggctgttcgc gtaaaaccac gtgcatgtct
     2701 gctaaatgag cggatgatgt ggtggggggc tcgatatatg ttcactaccc aacggccgac
     2761 cgccgcgggc gcggtcactg ccggcatact attcaccgct ctcacctgcc tgagcgcagg
     2821 ctatggcgcg gacaacctgg cagcccccac ggcgccggcg gcacctgcag ccatcgcgcc
     2881 tgccacaccg actgaagctg ccgcgcaatt gccccctccg cagtcagcgg aactgactga
     2941 gatcgtcatt cattcgaccg agccacgctt cgtgatgccg acgcgacgcg atcgcatcgg
     3001 ccggatctgg gcaccggtgt acatcaatgg gaaaggtcca ttccgactgg tgcttgattc
     3061 cggcgccaat cactcaggca ttaccgaaca ggtcgccgtc atcctgggct tgccgtccga
     3121 cagctcgcag caggtcatgc tgcgtggcgt gaccggctcg gcggcagtgc cgacggtacg
     3181 cgtcaacagc ttcgtcgtgg gcgatgtgca ggaaggggcc gagcgcctgc cgatcgtcac
     3241 cgatgcgctg ggcggcgccg agggcgtact gggtaccgac ggcatgcaga atcgccgtat
     3301 ctacatcgat ttccggcacg acctgatcag catcacacgt tcgaggagcg agcatgccga
     3361 tgcgggttac cgggttattc cgttcgagac catgcgcggc aacctggtcg tcgtggatgc
     3421 cacggtgggg ggaatacaca ccaaggccat catcgatacc ggtggtcagg tcacgatcgc
     3481 caatacggca ttgcggcaag cactcgagcg tcgcaagtca cagctactgg gccgggcgat
     3541 ccatattgaa ggggtcacga ccgacattca ggacgccgat gaaatcaata cgccgccgct
     3601 gcgaatcggc ggcgtggacg aaggcgttgc tagcgtgatc gagatccgct tccgggacat
     3661 cagcttcggc gacatgcgca tcttcgaaca ttggcgcctg accgatgagc cggcgatgct
     3721 ggtcggcatg gacgcgctgg ggctgctcga tacactgatc attgactacc ggcgtcacga
     3781 actgcagatt cgcatgcggc cgcaatagtc agccgcttcg cgccacgcct cgcgccatcc
     3841 gagtgcgcga gtcgagtttc gatggcgagt gaagcttgac gcagttcaac tggagcaacc
     3901 cctacccgag cacccgtgtg ccggtgttcg cccgcaatgt ggtctgcacg tcccatcctc
     3961 tggccgccca ggtgggcctg aaaatgctgt ggaagggcgg caatgcggtc gatgccgcga
     4021 tcgccgcggc cgcaacactc accgtggtcg agccagtttc ctgcggactg ggtggcgacg
     4081 ccttcgcgct cgtttgggac ggcacacagc tgcacggcct gaatgcctcc ggctacgcgc
     4141 cggccgcctg gtcggtggaa tactttcagc gcaaatacgg ccgcgatccg ggcatgagtc
     4201 agcgtcctat gcgcggctgg gataccgtga cggtccccgg tgtgatcgcc gggtggcagg
     4261 ccctgcatca gcgcttcggc acgttaccgc tggccgatat tctcgaaccg gccagtgaga
     4321 tcgcggaacg cggctacgcc gtgtcaccga tcgtcgcgca gaagtgggag gcagcggtac
     4381 cggaactgcg cacacagccc ggcttcgctg cgaccttcat gccggcaggg cgcgccccga
     4441 aggtcagcga actgatccgt ctgcccgatc acgcacgcac cctgcggatg cttggcctta
     4501 aaggcgctcg cgacttctac gaaggcgata tcgccgaaaa gattgcacag ttcgcgaagt
     4561 cggaaggcgc tgcgatgacg ctggatgatc tgcgaagctt ccgggccgag tgggtggagc
     4621 cgatctccag gacctatcgc ggctacacgg tgcacgagat cccgcccaac ggtcagggta
     4681 tcgctgcact gatggcgttg gggatcctca gtcaattcga tctgaaagcc ctggccgtgg
     4741 attcggtcga atcacagcac ctgcagatcg aggcaatgaa gcttgccttc accgacctgt
     4801 atcactacgt cgcggacccg cgctcgatgc aagtgacgcc ggcgcagttg ctcgacgacg
     4861 cctacctcgc ctcgcgcgcc aaactgatcc accgcagccg cgctggcgag tatcactgtg
     4921 gcatgcccgc gggcggtggc actatctata tgagcgcggc tgaccagaac gggatgatga
     4981 tcagcttcat ccagtccaac tacatgggat tcggctcagg agtggtggtc cccggaacag
     5041 gcatttcgct gcagaatcgc ggctatggat tttcgatgaa cccgcgttcg cccaacgccg
     5101 tcgccgctcg caagcgcccg tttcatacga tcattcccgc ctttctgacg cgtgacgtcg
     5161 gagttcagag gcaggcagtg atgagcttcg gggtgatggg cggggacatg caaccccagg
     5221 ggcatctgca gacgctggta cgcatgctgg actacgggca gcagccgcag gcggcatgcg
     5281 atgcaccgcg ctggaaactc aaccccgatt tcacgatcga tgtcgaagcg cagctcgacg
     5341 cgcgcacgct cgccggcctg cagtcatacg gtcacacggt caggtcgtac accgattctt
     5401 atatggactt cggttcaggt cagttcatct ggagactcga cccgcaagat gtgaatcgcg
     5461 gctacgtggc cgccagcgac agccggcgtg atgggctggc cgccggcatc taaggcccgg
     5521 cccgccctgc cgccatgcta ggctagttca ctcatcatcc gatcgcccga cgatggacga
     5581 cgaaccttcg gacatgccgc cggttgagat gccggcaacc gtgactacac ggcgccgagg
     5641 tggcgcgcgc gcggcggacg ccgagccggt cgcgcgggtc ggggatgtcc tgctgggacg
     5701 tttccgcctg cagcgaatca tcggtcgcgg aggcatgggg gtcgtgttcg ccgccaacga
     5761 cctgcagatg cagggcgagc cgctgatcgc gatcaagctg ctgaacgaag acatgcgcga
     5821 cttcccggtc gcggccatgg cactggagcg cgaatgccgc aaagtgcgca tgctggctca
     5881 cgacagcatc gtgcgggtgt tcgagttcta tcgcaccggc gagcagacct tcatcacgat
     5941 ggaactgctg gagggagaat ccctcgacgc cgtcatcaaa cgtttcccgc agggcgtcgc
     6001 gtggaacgat gcgtggatga tcattcgcgc cgcggcggac gcattggcgt acgcccaccg
     6061 ccagaagccg ccgttcgtgc attcggactt caagccgtcc aacgtgttct gcacccgctc
     6121 cgggcagatc aaggtgctcg acttcggtgt cgcacgcgcc gcgcgcacct cggacaaggc
     6181 cgctaccaac ctgagcattt tcgatccggc tcagtacggg gcactgactc ccgcgtacgc
     6241 gagtgcagag atgctcgccg ggctgaacgc tgacgtgcgc gacgacgtct atgcgctcgc
     6301 ctgcgtcacc tacgaggtac tgtgcggcga gcatcccttc gcccggcgtc cggcggatca
     6361 ggcgcgggcc atgatgctca agcccaagcc gatcgcatcg ctgggcggtc gcgtgaatcg
     6421 ggcactggcc cacggcctgg cggtcgaccg gatgcaccgt acaccgacgg ccgcgcagtt
     6481 catcgaggaa ctcggtgacc cgccggaacc gccggaggtg cagcggcgcc gatacctcaa
     6541 gtgggcgcgg gccggtgggg cgatcgccat cgcagtcatc gccggatttg gcctgtggcg
     6601 gctgaagccg ttgatccatt ggccgagtcg caccccatcg ctcgcaacca gttcggcgcc
     6661 ctcgccgagc cctgcaccgc tcgctgcgcc cgtgtccgcg tcccggcccg ttgcggccag
     6721 taaacgggcg acgcccgaac ctgcagagat ccgcagcccg gcctcgccgg gggcggttcc
     6781 cggcgatcgc ctgctcgcgc tgctgcaact gctgggtatc cccaatgacg tcaaacccac
     6841 taccacctat cctgagagcg cggcccgcaa gctgatcgag acctcgccac gcaaagtggt
     6901 ccttggcagt acgcccgaac agatgcaatc ggcactgacg ctttgccgcc gatattcctc
     6961 tgaatgccgg gcaagctggt atgccgacga gaaacagcgt accgcaacgc tcgaaccgtt
     7021 cgagctcgat cccactccgg tcagcgtgct cgaattcagg cgcttcgc
//
LOCUS       DATMRM010000242        14310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4169410, whole genome shotgun sequence.
ACCESSION   DATMRM010000242 DATMRM010000000
VERSION     DATMRM010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14310
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4169410"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VNZ06_06995"
     CDS             <1..630
                     /locus_tag="VNZ06_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20535.1"
                     /translation="QYLSGRGGELVEEMPLALVENQAYIHYRKGSVILYALKDYLGEA
                     VVDRTLARFDAEKAFQQPPYTTTREFLSDLRQEAGESWSPLIADLFDKITLFDNRVVD
                     AQAKRRSDGKYDVTLKLHATKFYADGVGKETAAKVDVPIDIGVFARAPDGKEQHEKVL
                     FLEKRTVADGDSTLTITVDGEPFEAGIDPYNKLVDRVADDNRRRVSLER"
     gene            808..1809
                     /locus_tag="VNZ06_07000"
     CDS             808..1809
                     /locus_tag="VNZ06_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20536.1"
                     /translation="MCPAPLVRSSPLSAPGTAALAFLYEHPQWFAPVFAELERRRVPF
                     DKFFIPDFWYDIGAAAPSFKVLFNRMSPSAHSRAHGSGIFHTLSFLEHLDRLGVRVIN
                     GAHAFRYEISKAAQHSLLRHLGLPYIASRVIHAPQQAIDAAKGLRFPILIKANIGGSG
                     KGIVRYDTAQALGAALAAGQVELGYDSIALVQEFVPARGGFITRVETLGGKYLYAIRV
                     HLSGQTFDLCPADICQTARGESLNTACVVEASKAGLKVEGYTPPADVIANIERIVRVA
                     GIDVGGIEYLTDDRDGQLYYYDVNALSNFVADAKNVIGFNPFENLADYLQQELSHAR"
     gene            1799..2839
                     /locus_tag="VNZ06_07005"
     CDS             1799..2839
                     /locus_tag="VNZ06_07005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWW20537.1"
                     /translation="MPADRIRFGYWMPVFGGWLRNVDDENMQASWEYTSHLARRSEHI
                     GYDLSLIAELNMNDIKGIDAPSLDAWSTSAALAAVTERLELMVAVRPTFHTPALFAKQ
                     AANIDHISHGRLALNVVSSWWEQEARMYGVDFERHDDRYGRTSEWLDVLDGVWKQKNF
                     SHAGRYYKTESTVLEPKPVRHPRPPIYAGGESEAAKNLIAQKCDAYVMHGDSPAHVKA
                     KIDDMRARRGKLGLGPMQFGVAGFAFTRDSDAAVKQELQRITDVKQNAKGYANYQQWL
                     QGTQLEQKMSIEEYSVSNRGLRSGLTGTPGAVQEQVGRFEEAGVNLLLLQFSPQLEEM
                     ERFSHSVIQPLQ"
     gene            2839..4767
                     /gene="thrS"
                     /locus_tag="VNZ06_07010"
     CDS             2839..4767
                     /gene="thrS"
                     /locus_tag="VNZ06_07010"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017402730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWW20538.1"
                     /translation="MPVITLPDGSQRTYDAPVSVADVASSIGAGLARAALGGRLNDKL
                     VDTSFVIHDDATLAIITDRDPEGIEIIRHSTAHLLAQAVKQLFPEAQVTIGPVIDNGF
                     YYDFAFKRPFTPEDLAAIEARMKELAKADLPVQRRVMPRDEAVKFFENIGEHYKAQII
                     AAIPAGEQISLYGQGDWVDLCRGPHVPSTGKLRAFKLMKLAGAYWRGDSRNEMLQRIY
                     GTAWADQKQLDAHLKMLEEAEKRDHRRIGRDLDLFHMQEEAPGAVFWHPKGWQVFQSL
                     IAYMRRKQGAAGYQEVNAPEVLDRSLWEASGHWAQFGENMYTTRTPDERVYAIKPMNC
                     PGHVQIFKQGIRSYHQLPIRFAEFGKVHRYEPSGALHGLMRVRAFTQDDAHVFVMEDQ
                     ITSEAAAMTHLILSIYKDFGFDEVRIKFSDRPTRRVGSDEVWDKAEAALAAASKAAGI
                     EYTLNKGEGAFYGPKLEFVLRDAIGRDWQCGTVQVDLNMPGRLGAHYIDEHSQRQTPV
                     MLHRAIFGSLERFVAILLEHCAGKLPAWIAPTQAVVMGITDRQHEYVEKTTDFLKKQG
                     LLVISDLRNEKVGFKIRDHTLQRVPYLLVAGDKEVAANQISVRSRSGKDLGAMSPQTF
                     VDRLRAEIDSYGRQTLED"
     gene            4771..5292
                     /gene="infC"
                     /locus_tag="VNZ06_07015"
     CDS             4771..5292
                     /gene="infC"
                     /locus_tag="VNZ06_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWW20539.1"
                     /translation="MAISAKRVRRNEEITSPTVRVIGADGGQAGVMTRAEALQLATAS
                     ELDLVEVSPTAEPPVVRVMDYGKFLFEQNKKAHSAKRKQKQIHVKEVKFRPGTEEADY
                     QVKLRNLIRFLSEGDKAKVTLRFRGREMVHQDIGRKLLDRVQIDLGPYSVVEQNPLME
                     GRQMVMLFAPKKK"
     gene            5405..5608
                     /gene="rpmI"
                     /locus_tag="VNZ06_07020"
     CDS             5405..5608
                     /gene="rpmI"
                     /locus_tag="VNZ06_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HWW20540.1"
                     /translation="MPKLKTNRAAAKRFRKTAKGWKKYSSGRRHNLGHKDSKRKRHAR
                     SGGSSLVHESDAHRIDQLMPNHK"
     gene            5621..5983
                     /gene="rplT"
                     /locus_tag="VNZ06_07025"
     CDS             5621..5983
                     /gene="rplT"
                     /locus_tag="VNZ06_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HWW20541.1"
                     /translation="MARVKRGVTAGRRHKKVLGKAKGYYNARRKVFRAAKQAVIKAGQ
                     YAFRDRRQKKRQFRALWIQRINAAARVHGLSYSRLIEGLTKAGFEIDRKILADLAMND
                     AKAFGVIASQAKAQLAAA"
     gene            6002..7027
                     /gene="pheS"
                     /locus_tag="VNZ06_07030"
     CDS             6002..7027
                     /gene="pheS"
                     /locus_tag="VNZ06_07030"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004979194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWW20542.1"
                     /translation="MGVQAMIEGALQEISRAGTLPELEEIRVRWLGKKGQITEQLKAL
                     GALDPAERKDAGARINAAKEQVQAAIEARRAELERLEVEHKLAAGRIDVTLPGRGEAL
                     GGLHPITQTRLRIEEIFRRAGFDVAHGPEIEDDFHNFGALNIPPNHPARAMHDTFYLH
                     NGLLRTHTSPVQIRVMQSSKPPIALIAPGRCYRHDPLDPTHSPAFHQVEGLYVDRNVS
                     FANLKSTLHKFSEAFFEQQLSIRIRSSFFPFTEPSAELDITCVLCRGRGCRTCKQSGW
                     LEIAGCGMVHPNVLNACGIDPEEYTGWAFGAGIERLAMLRYGISDLRHFFENDLRFLG
                     SLAGGAP"
     gene            7024..9450
                     /gene="pheT"
                     /locus_tag="VNZ06_07035"
     CDS             7024..9450
                     /gene="pheT"
                     /locus_tag="VNZ06_07035"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWW20543.1"
                     /translation="MKLALSWLREWVHIPPDWDARQLAARLTQGGFEVEAVLPAAGAF
                     SGVVVAKILSAEPHPQAGKLQVCRVSQGDDGSDSGSELQIVCGAPNARAGLVSALAKV
                     GASLPGDRHIGAAQLRGVDSHGMLCSARELGLGDDSNGILELPGDAALGIDLRKYLGL
                     DDSVLEINVTPNRGDAMSVLGIAREVAALAGTALKGNVTGTTLTPSMKQVSSSAEGDQ
                     FRLRTEAGAGAPRLLARSVRGLNNRLSSPLWLRERLRRVGLRSISPVVDVTNYVMLEL
                     GQPLHAYDLARLHGGLTARRARAGERLTLLDGREVELDPDVLVIADDKAAVGMGGVMG
                     GAATAISADTTDVLLEAAWFKPDVIAGRARRYGLFTDAGQRFERGVDWRGQERALALA
                     KRLILDIAGGTAGPVVTAELPDELPHHASVPLRYKRLERLLGASVAPSHVEHSLKSLG
                     LEISAISGGWTVQPPSWRFDVAIEADLIEEVGRVVGLDAIEEKAPLMLIEPRVLPANE
                     VESQVVLRTLAARGYQEVITFGFTDPVLQQTLFGSQPSIELINPISSDLAVMRSSLWP
                     GLISVARTNLQRQQTRVRVFEVAHRFLRDGEGHREQQMIAGLALGSRLPEQWGASATP
                     VDFHDVKDDLQSLFALCASAREFSFVPDALTCLHPGRSARVLRGGTPIGYLGELHPNL
                     VRSLDLTYTPILFELDYLGTFSAKLSQFREVSRFPQIRRDISVSVPEQVAYAALRERV
                     SLVSGDLLQQISLFDLYQGKGVEKGRKSVALGLILQDLSRTLTDEDADRVVHAVLADL
                     QSNLDARIRE"
     gene            9456..9755
                     /gene="ihfA"
                     /locus_tag="VNZ06_07040"
     CDS             9456..9755
                     /gene="ihfA"
                     /locus_tag="VNZ06_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HWW20544.1"
                     /translation="MALTKAEMAEALFNQLGLNKREARELVDLFFEEVRGALSQGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEEIPISARRVVTFRPGQKLKARVEAYAGTKE"
     gene            9736..10092
                     /locus_tag="VNZ06_07045"
     CDS             9736..10092
                     /locus_tag="VNZ06_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWW20545.1"
                     /translation="MLEPRNNNELPAIPGKRYFTIGEVSELCGVKPHVLRYWEQEFPQ
                     LKPVKRRGNRRYYQRQDVIVIRQIRSLLYEQGFTIGGARNKLGGDEARTDTTQSQQIV
                     RQLRSELEEVLRILRR"
     gene            complement(10089..11315)
                     /locus_tag="VNZ06_07050"
     CDS             complement(10089..11315)
                     /locus_tag="VNZ06_07050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HWW20546.1"
                     /translation="MKTSADSRRQAALRFIFVTVLIDMLAFGMIIPVLPVLVQDFMGG
                     DAARAAAMYGLFGTAWALMQFIFSPVQGSLSDHFGRRAVILISCAGLGLDFILMALAP
                     NLWWLLVGRVISGITAASFATAGAYISDVTPPEKRAAAFGMMGAAFGVGFILGPALGG
                     LLGAISPRLPFWASACMALANVCWGLFVLPESLPKERRVAFAWKNAHPLGALKLLRSH
                     RILAGLAVSYFLINLAHVVLPSTTVLYMHYRYGWDTRAVGILLAGVGISSLLVQGLLV
                     KPAVRWLAERRAMAVGLLFGAIGFAIYGLAFNGTLFWIGVPVMALWGIATPSLQTLMT
                     RLVSDTEQGRLQGALASLMGLASLIGPIMFTQIFAVSISTRADWGLPGAPFLLASALV
                     MGAVLLGWRTTAPQHN"
     gene            complement(11353..11676)
                     /locus_tag="VNZ06_07055"
     CDS             complement(11353..11676)
                     /locus_tag="VNZ06_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20547.1"
                     /translation="MSQRVREISVCILWFAAVALSGAYAIRQGQGSDELFLAMLTGSL
                     VALGTYDTRRNQPTQYNLLRSWPFGTPADDDSRGARTLPSHALHGMLWISVWLCLCQI
                     LYGHR"
     gene            11841..12590
                     /locus_tag="VNZ06_07060"
     CDS             11841..12590
                     /locus_tag="VNZ06_07060"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWW20548.1"
                     /translation="MQQSTVSITRADGTSMKAMYCLPDAPGGRLPAVLVIFDVFGMTT
                     DLSRIAGRFVQQGYAVLVPDLFDRPELRVLCVVRALRSTRRGSGREFDDLRAAREFLG
                     SRSEIDAARVAVTGFCMGGGFAVYLGASGLYKVSAPFYGDVPGNVEALRGTCPVIASF
                     GARDAKRMISAGHRLESHLKTLGVPHDVKFYPEAGHSFMNRNSGFLAERLAPNLPIHA
                     QYHEPSAEDAWRRLFSFFDAHMNRPSNPPAA"
     gene            12683..12759
                     /locus_tag="VNZ06_07065"
     tRNA            12683..12759
                     /locus_tag="VNZ06_07065"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12717..12719,aa:Pro,seq:ggg)
     gene            13388..13630
                     /locus_tag="VNZ06_07070"
     CDS             13388..13630
                     /locus_tag="VNZ06_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20549.1"
                     /translation="MNDNDATVRLRARWDGDTALAPEQAIELRELYSELQAATAAAAE
                     ALGKTGPMPAGMPLQRFREIDSRVAALVDRIRVILD"
     gene            13783..13995
                     /locus_tag="VNZ06_07075"
     CDS             13783..13995
                     /locus_tag="VNZ06_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20550.1"
                     /translation="MKVDTADMLSTLAIGDRVIFRSSKATACGTVTQVLDDGVVRVLW
                     DGEERGPLQFGQVLVKLMPELHHPAA"
     gene            14081..>14310
                     /locus_tag="VNZ06_07080"
     CDS             14081..>14310
                     /locus_tag="VNZ06_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20551.1"
                     /translation="MTDDTETERLPRLKGLGNRLTAAELDELRELYWELPSATEAAAE
                     LLGSQGPMPTGMRFERFMEVTERVAALIVEIDT"
BASE COUNT         2795 a         4319 c         4644 g         2552 t
ORIGIN      
        1 cagtatctga gcggccgcgg cggcgagctg gtcgaggaga tgccgctcgc gctggtcgaa
       61 aaccaggcgt acatccacta tcgcaagggt tcggtgatcc tgtacgcgct caaggactat
      121 ctgggtgagg cggtggtcga ccgcacgctg gcgaggttcg atgccgaaaa ggcgttccag
      181 cagcccccgt acaccacgac ccgggagttt ctcagcgatc tgcgccagga agccggcgaa
      241 tcatggtcgc ctctgatcgc cgatctgttc gacaagatca ccctgttcga caaccgggtc
      301 gtcgacgccc aggcaaaacg gcggtcggac ggcaaatatg acgtcacgct caagctgcac
      361 gcgaccaagt tctatgccga cggggtgggc aaggagacgg ccgccaaggt ggacgttccg
      421 attgatatcg gggtattcgc gcgcgccccc gatggcaagg aacagcacga gaaggtgctg
      481 tttctggaaa agcgcaccgt tgccgatggc gatagcaccc tgacgattac cgtggacggc
      541 gagccgtttg aggccggcat cgacccgtac aacaagctgg tggatcgcgt cgctgacgac
      601 aaccgcaggc gggtcagcct cgagcgctga ttggggatta ggcctaaagt gtccttgaga
      661 tgctccgcct ccgccgcgcg cacccgttcg cgacggcaaa tgcccctggc ctgtcaactt
      721 tcggtgttct gcggttccgc gggtgcagcc ctcgaggctt tctgccaaaa ttctcgtttg
      781 tcccccgtcg ctccgaggtt cccttaggtg tgtcctgctc cgctcgttcg ttcatcaccg
      841 ctgagtgcgc cagggactgc agcgctcgcg tttctgtatg agcacccgca atggtttgcg
      901 ccggtgttcg ccgagctcga gcgtcgccgc gtgccgttcg acaagttctt cataccggat
      961 ttctggtacg acatcggcgc cgccgcgccg tcgttcaagg tgctgttcaa ccgcatgagc
     1021 ccctcggcgc acagccgagc gcacggctcg ggcatctttc acacgctaag cttcctggag
     1081 catctggatc ggctcggcgt gcgcgtcatc aacggtgccc acgcctttcg gtacgagatt
     1141 tccaaggccg cgcagcactc gctcctgcgc cacctggggc tgccgtacat tgcctcgcgc
     1201 gtcatccacg cgccgcagca ggcgatcgat gcggccaagg gccttcgctt tccgatcctg
     1261 atcaaggcga acatcggcgg cagcggcaag ggcatcgtgc gctacgacac cgcgcaggcc
     1321 ctgggggcgg cgctcgccgc cggccaggtg gaactgggtt acgacagtat cgcgctggtg
     1381 caggaattcg tgccggcgcg cggcggattc attacccggg tggagaccct cggtggcaag
     1441 tacctgtacg ccatccgggt gcacctgagc gggcagacct tcgatctgtg cccggccgac
     1501 atctgccaga ctgcgcgcgg cgagagcctg aacaccgcct gcgtggtgga ggcctcgaag
     1561 gccggcctga aagtggaggg ctacacgccg ccggccgatg tgatcgcgaa catcgagcgc
     1621 atcgtgcgcg tcgccggtat cgacgtcggc ggcatcgaat acctgaccga cgaccgtgac
     1681 ggtcagctct actattacga cgtcaatgcg ctgtcgaact ttgtggccga tgcgaagaac
     1741 gtgatcggct tcaacccgtt cgagaacctc gccgactacc tgcagcagga gttgtcgcat
     1801 gcccgctgac cgcatccgct tcggctactg gatgccggtg ttcggcggct ggctgcgcaa
     1861 cgtcgacgat gagaacatgc aggcgagctg ggaatacacc agccacctcg cgcgccgcag
     1921 cgagcacatc ggctacgacc tgtcgctgat cgccgaactc aacatgaacg acatcaaggg
     1981 gatcgacgcc ccgagcctgg atgcctggtc tacctcggcc gcgctggccg cggtgaccga
     2041 gcggctggag ctgatggtgg cggtgcggcc gacgtttcat acgccggcgc tatttgccaa
     2101 gcaggcggcg aatatcgatc acatcagcca cgggcggctc gcactgaacg tggtctcttc
     2161 gtggtgggaa caggaagccc gcatgtacgg cgtggacttc gagcgccacg acgaccgcta
     2221 cggccgcacc tccgagtggc tggatgtcct ggacggcgtc tggaagcaga agaatttcag
     2281 tcacgccggc aggtactaca agacagaaag cacggtgctg gagccgaagc cggtgcgcca
     2341 tccgcgcccg ccgatctatg ccggcggtga gtcggaggca gccaagaacc tgatcgcgca
     2401 gaaatgcgat gcctacgtga tgcatggcga ttcgcccgcg cacgtgaaag cgaagatcga
     2461 tgacatgcgc gcccggcgcg ggaaattggg actgggcccg atgcagttcg gcgttgccgg
     2521 ttttgccttc acccgcgact cggatgcagc ggtcaagcag gagctgcagc ggatcacgga
     2581 cgtcaagcag aatgcgaagg gctatgcgaa ctatcaacaa tggcttcagg gtactcagct
     2641 cgaacagaaa atgtcgatcg aggagtattc ggtttcaaat cgcggcttgc gctcgggact
     2701 cacgggtacc cctggcgcgg ttcaggagca ggttggccgc tttgaggaag cgggcgtgaa
     2761 cctgctgctg ctgcagttca gcccgcagct cgaggagatg gagcgcttca gccacagcgt
     2821 catccagccg ctgcagtaat gccagtcatc actctgccgg acggcagcca acgcacctac
     2881 gacgcgccgg tcagcgtcgc cgatgtcgcc tccagcatcg gcgccggtct cgcgcgcgcc
     2941 gcgctcggcg gccgcctcaa cgacaagctg gtggatacct cgttcgtcat ccacgacgac
     3001 gcaacactgg cgatcattac cgaccgcgat ccggaaggca tcgagatcat tcgccattcc
     3061 accgcgcacc tgctggcgca ggcagtgaag cagctctttc cggaagcgca ggtcacgatc
     3121 gggccggtga tcgataacgg cttctactat gacttcgcgt tcaaacggcc gttcacgccg
     3181 gaggatctgg ccgcgatcga agcacgcatg aaggagctgg cgaaagccga tctgccggtg
     3241 cagcgccgtg tgatgccgcg cgatgaggcg gtgaaattct tcgagaatat cggcgagcac
     3301 tacaaggcgc agatcatcgc ggcgatcccc gctggcgagc agatcagcct ctacggccag
     3361 ggcgactggg tggacctgtg tcgcggtccg catgtgccat cgaccggcaa actacgcgcg
     3421 ttcaagctga tgaaactcgc tggcgcctac tggcgcgggg attcgcgcaa cgagatgctg
     3481 cagaggatct acggcaccgc ctgggcggac cagaagcagc tcgatgcgca cctgaagatg
     3541 ctcgaggaag ccgagaagcg cgatcaccgc cgcatcggcc gcgacctgga tctgtttcac
     3601 atgcaggagg aggcgcccgg ggcggtattc tggcatccga agggctggca ggtgtttcag
     3661 tcactgatcg cctacatgcg ccgcaagcag ggcgcagcgg gctatcagga agtgaacgcg
     3721 ccggaggtcc tggaccgaag cctgtgggag gcgtccggcc actgggcgca gttcggggag
     3781 aacatgtaca ccacgcgcac ccccgatgag cgcgtgtatg ccatcaagcc gatgaactgc
     3841 ccggggcatg tgcagatttt caagcagggt atccgcagct atcaccagct gccgatccgc
     3901 ttcgccgagt tcggcaaggt gcaccgctac gagccgtccg gtgcgttgca tgggctgatg
     3961 cgggtgaggg cctttaccca ggatgatgcg catgtattcg tcatggagga tcagatcacg
     4021 tccgaggcgg ccgcgatgac tcacctgatc ctctccattt ataaggattt cgggttcgat
     4081 gaggtgcgca tcaagttctc cgaccgcccg acacggcgcg tgggctcgga tgaggtctgg
     4141 gataaagccg aggccgcgct ggcggcagcg tccaaagcgg ccggcattga gtacacatta
     4201 aacaaaggcg agggagcctt ttacgggcca aagctagaat ttgtcttaag agatgcgatt
     4261 ggccgggact ggcagtgcgg taccgtacag gtggacctga acatgcccgg aaggctgggc
     4321 gcgcactata tcgatgagca cagccagcgg cagactccag tcatgctgca ccgggcgatt
     4381 tttggatcgc tcgagcgttt tgtggccatt ctgctcgagc actgcgccgg caaactgccc
     4441 gcctggattg caccaactca ggccgtggtg atggggatca ccgaccgcca gcatgagtat
     4501 gtcgaaaaga ctacagattt cctgaagaaa caagggcttc tggtcatatc tgacttgcgc
     4561 aacgagaagg tcggatttaa gatccgcgac cacacgctgc agcgggtccc ctatcttctg
     4621 gtcgccggag ataaagaggt tgcagcgaat cagatttctg tgcgctcccg tagtggaaaa
     4681 gatctgggcg cgatgtcgcc gcagacgttc gtggatcgtc tgcgcgccga gattgatagc
     4741 tacgggcgcc aaactttgga ggattgatat atagccattt cagcgaagcg agtccggcgt
     4801 aacgaagaaa ttacctcgcc cacggtgcga gtgatcggag cggatggggg gcaagcagga
     4861 gtcatgacgc gcgcggaagc gctgcagttg gccactgctt ctgaattgga tctggttgaa
     4921 gtctcgccga cggcggaacc gccggtcgtg cgggtgatgg actatggcaa attcctgttc
     4981 gaacagaaca agaaggcgca ttctgccaag cgcaagcaga agcagataca tgtcaaggaa
     5041 gtgaagtttc ggcccggcac ggaagaggcg gattaccagg tcaagctgcg taatctgatc
     5101 cggtttctgt ccgagggcga taaggccaag gtgaccttga ggttccgagg ccgcgagatg
     5161 gtgcatcagg acatcggccg caagctgctg gatcgggtac agatcgatct tggcccgtat
     5221 agcgtggtgg aacaaaaccc gctgatggaa ggccggcaga tggtgatgct gtttgcaccg
     5281 aaaaagaagt gaccgcgcgt ccggcagcta gcgctgcctg acgctcggtc cgccagtgag
     5341 cgggtcatcg tggcgagagc ccgacggctt tgtgaactgc aggcaacaac gagtaggagt
     5401 gatcatgccc aagttgaaaa ccaaccgggc ggcggcgaag cgttttcgca agacagccaa
     5461 gggctggaag aaatattctt cgggtcgtcg ccataacctc ggccataaag attccaagcg
     5521 caagcggcac gcgcgctcgg gcggcagcag cctggtacac gagagcgatg ctcatcgtat
     5581 cgaccagttg atgcccaatc acaaatagca gaggttcacg atggcaagag tcaaacgggg
     5641 tgtcaccgcg ggtcggcgac acaagaaggt ccttggcaag gccaaggggt attacaacgc
     5701 gcggcgcaaa gtcttccgcg ccgccaagca ggcggtcatc aaggccggcc agtatgcgtt
     5761 ccgcgaccgg cgccagaaaa agcgtcagtt ccgcgcgctg tggattcagc gcatcaacgc
     5821 ggccgcgcgc gtgcatggcc tgtcgtacag ccgcctgatc gaagggctca ccaaggcggg
     5881 cttcgagatc gatcgcaaga tcctggcgga tctggcgatg aacgatgcca aggcattcgg
     5941 cgtgatcgct agccaggcaa aagcccagct ggccgcggcg taagcgcgct tgccgtttgc
     6001 gttgggggtg caggccatga ttgagggcgc actgcaggag atctcgcgcg cgggcacgtt
     6061 gcccgagctg gaggagatcc gtgtccgctg gctgggcaag aagggccaga tcaccgagca
     6121 gctcaaagcg ctcggcgcgc tcgatcctgc cgagcgcaag gacgccggcg cgcgcatcaa
     6181 tgcggcgaaa gaacaggtcc aggccgcgat cgaagcgcgc cgcgccgaac tcgagcggct
     6241 cgaggtcgag cacaagcttg cggccggccg gatcgatgtg acgctgcccg ggcgcggcga
     6301 ggcgctcggc ggactgcatc cgatcacgca gacccggctt cggatcgagg agatcttccg
     6361 gcgcgcgggc ttcgacgtgg cgcacggtcc ggagatcgaa gacgacttcc acaacttcgg
     6421 cgcgctcaac attccgccca atcatccggc gcgcgcgatg cacgatacct tctatctgca
     6481 caacggcctg ctgcgcacgc atacctcgcc ggtgcagatc cgcgtgatgc agagctccaa
     6541 gccgccgatt gcactgatcg cgccgggccg ctgttaccgg cacgatccgc tggacccgac
     6601 gcattcgccg gcgtttcatc aagtcgaggg cctgtacgtc gatcgcaacg tcagtttcgc
     6661 gaacctgaaa tccacgttgc acaagttcag cgaagccttc ttcgagcagc agctctcgat
     6721 ccgcatccgc tcctcgttct tcccgttcac cgagccctcg gccgaactgg atatcacctg
     6781 cgtgctgtgc cggggccgag gttgccgcac ctgcaaacaa agcggatggc tggagattgc
     6841 cggctgcggc atggtgcacc cgaacgtact gaacgcctgc ggcatcgatc cggaggaata
     6901 caccggctgg gctttcggcg ccggcatcga gcgcctggcg atgctgcgct atgggatcag
     6961 cgatctgcgc cacttcttcg agaacgatct gcgtttcctg ggatcactgg ccggaggggc
     7021 gccgtgaagc tggcactgtc ctggctgcgc gaatgggtgc acatcccgcc ggactgggat
     7081 gcgcgccaac tggctgcgcg gctgacgcag ggcggattcg aagtcgaagc ggtgctgcct
     7141 gcggccggcg ctttctccgg cgtcgtggtc gcaaagattc tgtccgccga gccgcatccc
     7201 caggccggga agcttcaggt gtgccgtgtc tcgcagggcg acgacggctc ggacagcggc
     7261 agtgaattgc agatcgtgtg cggtgcgccc aatgcgcgcg ccggactggt cagcgcgctg
     7321 gcgaaggtcg gcgcgagtct gccaggagac aggcacatcg gtgccgcgca gctgcgcggc
     7381 gtggactcgc acggcatgct ctgttcggcg cgggaactgg gcttggggga tgacagcaac
     7441 ggcatcctgg aattgccggg cgatgccgcg ctcggcatcg acctgcgcaa gtacttaggg
     7501 ctggacgatt cggtgctgga gatcaatgtc acgcccaatc gaggcgatgc gatgtcggtg
     7561 ctgggcattg cgcgcgaagt ggcggcgctc gccggcactg cgctcaaggg caatgtcact
     7621 ggcaccacgc tcacgccatc gatgaaacag gtgagttcga gcgccgaagg cgatcagttc
     7681 cgtctgcgca ccgaagccgg tgcgggtgcg ccgcgcctgc tcgcgcgcag cgtgcgcggt
     7741 ctgaacaacc ggctttcaag tccgctgtgg ctgcgcgagc gcttgcggcg cgtgggactg
     7801 cgctcgatca gcccggtggt ggacgtcacc aactatgtga tgctcgaact cggtcagccg
     7861 ctgcacgcct acgacctggc gcggctgcac ggtgggctca cagcgcgtcg cgcgcgcgcc
     7921 ggggagcgac tgacgttgct ggatgggcgc gaagtggaac tcgatcccga cgtgctggtg
     7981 atcgcggatg acaaggccgc ggtcggcatg ggtggggtca tggggggtgc ggcaacggcg
     8041 atcagcgccg acaccaccga cgtgctgctg gaggccgcgt ggttcaagcc ggacgtcatc
     8101 gccggccggg cgcgccgcta tggactcttc accgatgctg gacagcgctt cgagcgcggc
     8161 gtggactggc gcggacagga gcgtgcgctc gcgctcgcca agcggctgat cctcgacatc
     8221 gccggcggta ccgccggacc ggtggtcacg gcggaactgc cggacgaact gccgcatcac
     8281 gccagcgtgc cgctgcgcta caagcgactc gagcgcctgc tcggcgcgag cgtcgcgccc
     8341 agtcatgttg agcacagtct gaaaagcctg gggctggaga ttagtgccat cagcggcggc
     8401 tggacggtgc agccgccctc ctggcgtttt gacgtcgcga tcgaagcgga cctcatcgag
     8461 gaggtcgggc gcgtggtcgg cctcgatgcg atcgaggaaa aagccccgct gatgctgatc
     8521 gagccgcgcg tgctgccggc caatgaagtc gaatcgcagg tggtgctgcg cacgctggcc
     8581 gcgcgtggct accaggaagt cattaccttc ggcttcaccg acccggtgct gcaacagacg
     8641 ctgttcggct cgcagccgag catcgaactc atcaatccga tttcgagcga tctggccgtg
     8701 atgcgctcat cgctgtggcc gggattgatc agcgttgcca gaaccaacct gcagcgccag
     8761 cagacgcgtg tgcgcgtgtt cgaagtcgcg caccgattcc tgcgtgacgg tgagggacat
     8821 cgtgaacagc agatgatcgc agggctcgcg ctgggctcgc gcctgccgga gcagtggggc
     8881 gcgagcgcga cgccggttga tttccatgat gtgaaggatg atctgcagag cctgtttgcg
     8941 ctgtgtgcca gcgcgcgcga gttttcattt gtaccggacg cgctgacgtg cctgcatcct
     9001 gggagaagtg cgcgcgttct gcgcggcggg acgccgatcg gctacctcgg cgagctgcac
     9061 cccaatctgg tgcgttcact cgacttaaca tataccccga tcctgttcga actagactat
     9121 ttaggcactt ttagcgccaa actttcgcag tttcgcgaag tttcgcgctt cccgcagatc
     9181 cggcgggata tttccgtatc ggtgcccgag caggttgcct acgctgcctt gagggaacgt
     9241 gttagccttg tatccggcga cttgctccaa caaataagcc tcttcgacct ctaccagggt
     9301 aagggagtag aaaaaggtcg aaaaagtgtg gccttaggat tgattttaca ggatttaagt
     9361 cgcacgctta cagacgaaga tgcagaccgc gtggtgcacg cagtgctggc agatttgcag
     9421 tcgaatttgg atgcgcgaat aagagaataa cggcgatggc actgaccaaa gcggagatgg
     9481 ccgaggcact gttcaaccag ctcggtctga acaagcgaga agcacgcgaa ctggtagatc
     9541 tcttcttcga agaggtgcgt ggagcgttgt cgcagggaga gcaagtgaag ctgtctggct
     9601 tcggcaactt cgacctgcgc gacaagaatc agcgaccagg gcgcaacccc aaaaccggtg
     9661 aggaaattcc cattagcgcg cgccgcgtgg tgacattccg ccccgggcaa aaactgaagg
     9721 cgcgcgtcga agcctatgct ggaaccaagg aataataacg agctgccggc cattcccggc
     9781 aaaagatatt ttacgatcgg ggaagtgagc gagctgtgtg gagtgaaacc acacgtactg
     9841 cgctactggg aacaggagtt tccgcagctc aagcccgtca agcgtcgtgg caaccgtcgt
     9901 tactatcagc gccaggacgt gatcgtgatc cgccagatcc gaagcctgct gtacgagcag
     9961 ggcttcacga tcggtggcgc gcgcaacaaa ctgggcggcg atgaggcgcg gaccgacacc
    10021 acgcagagcc agcagatcgt gcgacagctg cgctccgagc tggaagaggt cctgagaatt
    10081 ctgcgtcgct agttgtgttg tggcgccgtc gtgcgccagc ccagcaacac cgcgcccatt
    10141 accaaggccg atgccagcag gaatggcgcg cccggcaatc cccagtccgc gcgcgtactg
    10201 atggatacgg cgaagatctg ggtaaacatg atgggtccga tgaggctcgc cagccccatg
    10261 agactcgcca gcgcaccctg caggcgccct tgttccgtgt cactcaccag ccgcgtcatg
    10321 agtgtctgta gcgatggcgt cgcgattccc cacagcgcca tgaccggcac accgatccag
    10381 aatagggttc cgttgaaggc caggccgtag atggcgaagc caatcgcgcc gaacagcaga
    10441 ccgaccgcca ttgcccttcg ttcggcgagc catctgactg ccggcttcac cagcaggccc
    10501 tgcaccagca gcgatgagat gcccacaccg gccaatagga tccccacggc gcgggtatcc
    10561 cagccatagc gatagtgcat gtacagcacc gtcgtgctcg gcaacacgac atgcgcgaga
    10621 ttgatgagga aatagctgac cgccaggccc gctagtatcc ggtgcgaacg caacagcttc
    10681 agcgcaccca gcggatgggc gttcttccag gcgaacgcga cgcggcgttc cttcggtaat
    10741 gattcgggca acacgaacag tccccagcac acattggcga gcgccatgca ggccgaggcc
    10801 cagaacggca ggcgcggtga gatggcgccg agcagaccgc ccaatgccgg gccgagtatg
    10861 aagccgacac cgaacgcggc gcccatcatt ccaaacgccg cggcgcgctt ttccggtggc
    10921 gtgacatccg aaatataggc tcccgccgtg gcgaaactcg ccgcggtgat gccggagatc
    10981 acccggccca ccagcagcca ccacaggttc ggtgccagtg ccatcaggat gaaatccaga
    11041 cccagacccg cgcaggagat caggatcacc gcgcggcgcc cgaaatgatc cgacagcgag
    11101 ccttgtaccg gggagaagat gaattgcatc agcgcccagg cggtgccgaa cagtccgtac
    11161 atcgcggccg cgcgcgcggc atccccgccc atgaagtcct gtaccagcac cggcagcacc
    11221 ggaatgatca ttccgaaggc cagcatgtcg atgagtacgg tgacgaagat gaatctcagc
    11281 gccgcctggc ggcgcgagtc ggcggatgtc ttcacaccgc aagtttacta gagcgctgcc
    11341 gaacctgggt cgctaacggt gtccgtacag aatctggcac aggcatagcc agaccgatat
    11401 ccacagcatg ccgtgcagcg cgtgcgaggg aagggtgcgg gcaccgcggc tgtcatcatc
    11461 ggccggcgtg ccgaacggcc aggagcgcag caggttgtat tgcgtcggct ggttcctgcg
    11521 tgtgtcatac gttccgagtg cgacgagcga gccggtcagc atcgccagga aaagctcatc
    11581 cgatccctgg ccctggcgga tcgcataggc accggacagg gccacggcgg cgaaccacag
    11641 tatgcagaca gaaatttccc taaccctctg actcatatca tcccttccac ggtgcgcggg
    11701 tgaccgcgcg cttgccggtc tttgccggtc aatttacacg tgaattgttt tttcgccgcg
    11761 accgatgtcg caggatcgac ttgacgtcgc gcgcacgatt gaagtcttct aagcgacgca
    11821 gtatccaggg gatctgagcg atgcagcagt caaccgtctc catcacgcgc gctgatggta
    11881 cttcgatgaa agcgatgtac tgtctgccgg atgccccggg cgggcgcctg cccgcagtgc
    11941 tggtgatctt cgatgtcttc ggcatgacga ccgatttgtc gcgcatcgca gggcgcttcg
    12001 tgcaacaggg ctatgcggtg ctggtgccgg acctgttcga tcgaccggag ctgcgcgtgc
    12061 tgtgtgtggt gcgggccctg cgctccacgc ggcgtggcag cggtcgcgag ttcgatgatc
    12121 tgcgggccgc gcgcgaattc ctgggcagcc gcagcgagat cgacgctgcg cgtgttgccg
    12181 tcaccggctt ctgcatgggc ggaggtttcg cggtgtatct cggggcgagc gggctctaca
    12241 aagtgagcgc tccgttctat ggcgatgtac ccggcaatgt cgaggcgctg cgcggcacct
    12301 gtccggtcat tgccagcttc ggggcgcgcg atgcgaagcg gatgatctcg gcggggcatc
    12361 ggctcgagtc gcacctgaag acgctcggtg tgccgcacga tgtgaagttc tatcccgagg
    12421 ccggtcacag cttcatgaat cgcaacagtg ggtttctggc tgaaaggctg gccccgaatc
    12481 tgccgattca cgcccagtat cacgagcctt ccgcggaaga tgcctggcgg cggctattct
    12541 cgttcttcga cgcccatatg aaccggccgt cgaatccgcc agcggcctga ctgacccgcg
    12601 accccgccgg ccggtcaaac cgggtatcat ggccgcacgc ggtaccgtgg gaccggtgcc
    12661 gcgcaagaag aataaatcgt gtcggagcgt ggcgcagtct ggtagcgcac ttgcatgggg
    12721 tgcaaggggt cccgagttca aatctcggcg ctccgaccat ttaaatcgtt gtctattccg
    12781 aagacatcgg taacctatcc atcaggtagg gcatcgggtc tagccagcgc tcacgctcgg
    12841 gcactggtgg aaccgttccg tgatcgtatc cggtaggggt tctattgggc tcaaagacaa
    12901 atccagagag accaccggat gcccttcaaa ttcgccgctc ccagcaacgg cctgcggtgc
    12961 gtcaacaaaa ccgccgacca cttcgcagta cacaagccat agagctaccg gcccgtagat
    13021 aaactttggc gctttggtat gaccctccgg actatgcacg gcaatctttg cccgccagcc
    13081 tggattggca tgcaccgctg taaacttcgg ctggttcgcc ggcatgtgcg ctgtgccgtc
    13141 ggcaaaaaat cccgctggcg cgcattaccg cgccagcggg tgaatactga gtccatcgat
    13201 catcgctcgg gggtcgagtg atggtctact agggaggact ggactcaggc gtgcacatta
    13261 ctcggccaag gtgggcgcag tcggtacggt gccccacacg gcctgccatt aagtaatgcg
    13321 acggcccgtg ccgagtgtga gatgagtcgt ccgagacgcc tcgggtggag gcgtaaaatt
    13381 cggctctatg aatgacaacg atgcaacggt acgacttcgc gctcgctggg acggcgacac
    13441 tgctctcgcg ccagagcagg cgatcgaact tcgcgagctt tactcggagc tgcaggccgc
    13501 taccgcggcg gcggctgaag cactgggtaa gaccggccca atgccggcgg gcatgcccct
    13561 tcagcgattc cgggagattg actcccgcgt tgccgcgctt gtcgatcgga tccgagtgat
    13621 cctcgactaa gccccgccct tcccacaggg ccgcgggctt tttctttgcg atgagccata
    13681 tgtgagcgag gtcacatcct aatatcggaa actacgtatg tgccggcaca ggcgcacggt
    13741 cgatacctgc gttaggtcta cataagcgcg ggggcgcagg acatgaaagt ggacacagca
    13801 gacatgctca gtacgctggc catcggagat cgggtcatct tccgcagctc gaaagcaact
    13861 gcgtgcggta ccgtcactca ggtactcgac gacggggtcg tgcgcgtgct gtgggatggg
    13921 gaagagcgcg gtccgctgca gttcggccaa gtgctcgtca agctgatgcc tgagctgcac
    13981 cacccagcgg cctgaagcca gacctcctga gcgaccactc gctttcattt gtgaggcgcg
    14041 tcatgcagtg cctcaaagac gcagcgtacg attccggcaa atgacggacg acaccgaaac
    14101 cgaacgcttg ccgcgtctaa aaggcttggg gaatcgcctg acggccgccg agctggacga
    14161 actgcgagaa ctctattggg agctgccgtc tgccaccgag gcggcagccg agttgctggg
    14221 ctctcaaggg ccgatgccta ccggtatgcg atttgagcga ttcatggaag tcaccgagcg
    14281 agttgccgcg ctgattgtcg agatcgatac
//
LOCUS       DATMRM010000243         3523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2619099, whole genome shotgun sequence.
ACCESSION   DATMRM010000243 DATMRM010000000
VERSION     DATMRM010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2619099"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VNZ06_07085"
     CDS             complement(<1..842)
                     /locus_tag="VNZ06_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWW20552.1"
                     /translation="MSARDIHPGRASAPSTLAPLLWLTMIGHVYAASQPPSRAPTIAD
                     LDSKAVTVQRDSKPAAGGASKAMENYRQFLELQNADPRLRAEALRRLGDLNLESGELE
                     RMANEVTQVDRQGAEAIRLYSTLLKAYPNYPRNDQVLYQLARAYETTGQSDLALTTLD
                     GVVVRYPQSRDIAEVQFRRGELLFSNRRYADAQRAYEVVLMHGRTGSSFYAQSLYKHG
                     WSQFKQGQNEDSLKSFGDLLDLTLSDPKDSSRMRSLESLGRADRELLEDTLRVMSITF
                     SYLDG"
     gene            complement(839..2797)
                     /locus_tag="VNZ06_07090"
     CDS             complement(839..2797)
                     /locus_tag="VNZ06_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20553.1"
                     /translation="MRFRRRSLSLACASLLVAATPVAWAKHRPDPNKLPSIKVQDLHY
                     GDVLFHDWADEDSGLQTLTLLEAYSQWNRMPHHDADAQLLAAGLYLQLGMHNEAGRRF
                     ETMLGERLPPAVRNQAWFYLAKIWYERGYYDRSEQALSHINGQLQPQPEAERVHLLVN
                     VLMRQQRFDEAIAILKNWKGPADWMAYARFNLGVAMVRAGRLVEADPILTAVGTLNTY
                     SPELLNLRDKANLALGYAYLQADQPAPALIPLARVRLEGPYSSRALLGDGWARAALKD
                     YRAALAPWLELHKRGLLDAAVQESYLAVPYAYGQLGAGGQAAEAYEDALKSFSSETDS
                     LDNAIAHIRGGHLLDDLLASDKDNANTYGWFYQLRTLPNSPQSRYLYALLADNDFQEG
                     LKNWRDLGYLQRSLLRWDDSMDAFGAMIDTRERAYAGLLPRADALLASNAPARLSGER
                     GAADSRLNAIEIGNDVAALGTPPERDQWARILHLEEVAASMPANSENAQSLEKLRLIK
                     GVLYWRLDSAFKQRSYQQRHELQELDAQLEELQNRWVRVQRARATMPTNTGDFAARIA
                     ALAERIKSVRQRLGDSARQQSQYLASLAEAELQSQRDRLSTYAVQARVQLADIYDRSA
                     DTNEKPSDSAARPAGESIPEQKSVPEQK"
     gene            complement(2805..3428)
                     /locus_tag="VNZ06_07095"
     CDS             complement(2805..3428)
                     /locus_tag="VNZ06_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20554.1"
                     /translation="MNTTLRLLTAAACLSAGMVYAQQPPASTPPATTPPAASPDAAVA
                     PPSTSDSDTAPSADSTRALDQQIQVLKKDVVDLNKDLFVLEEELLFPANTQVAVFVSV
                     DVGDFFALDSITLKIDNRDVMNYLYTPRETAALLKGGVQRLYVGNLKAGEHELVAFFN
                     GKGPHDRNYKRGANLKFEKGVGAKYLELKVTDHAHAQEPEFEIKDWE"
BASE COUNT          618 a         1109 c         1107 g          689 t
ORIGIN      
        1 ccatccagat aggagaacgt gatgctcatg acgcgcaggg tgtcctccag cagctcgcga
       61 tcggcgcggc cgagcgattc caagctgcgc atgcgtgagc tgtccttggg gtcgctcaac
      121 gtcagatcga gcaaatcgcc gaaggacttc aggctgtctt cgttctggcc ctgcttgaat
      181 tgcgaccagc catgcttgta gaggctctgc gcatagaagc tcgatccggt gcggccgtgc
      241 atcagcacaa cttcgtacgc ccgctgagcg tcggcatagc gacgattgga gaacagtagc
      301 tcgccgcgcc ggaactgcac ttcggcgatg tcgcgactct gcgggtagcg cacgacgacg
      361 ccatcgagcg tggtgagcgc cagatcgctc tgtccggtcg tctcgtaggc gcgcgccagt
      421 tgatagagga cctggtcgtt gcgcgggtag ttcgggtagg ccttgagcag cgtcgaatac
      481 aggcgaatcg cctcggcgcc ctgacgatct acctgcgtga cctcattggc catgcgctcg
      541 agttcgcccg actcgagatt cagatcgccc agccggcgca gagcctcggc gcgcagcctc
      601 ggatccgcat tctgcaactc gagaaactgc cggtagttct ccatggcctt ggaggcgcca
      661 ccggctgcgg gcttgctgtc gcgctgaacc gtaacggctt ttgaatcaag atcggcgatc
      721 gtaggtgccc tggacggcgg ctgcgaggcc gcgtacacgt gtccgatcat cgtcagccac
      781 agcagcggcg ccagcgttga cggcgccgat gctcgacccg gatggatgtc gcgcgcgctc
      841 atttctgctc gggcaccgac ttctgttcag ggatgctctc gccggccggt ctcgcggctg
      901 aatcggaagg cttctcgttc gtgtctgcgg agcgatcgta gatatcggcg agctgcacgc
      961 gcgcctgcac cgcgtaggta gacagacgat cgcgttgcga ttgcagttcc gcttccgcga
     1021 gactggccag gtactgactc tgctgccgtg cagaatcgcc caggcgctga cgcaccgact
     1081 tgatgcgctc ggcaagcgcc gcaatgcgcg cggcgaaatc cccggtattg gtcggcatcg
     1141 tggcgcgagc gcgttgcacg cgcacccagc gattctgcag ttcctccagc tgcgcatcga
     1201 gctcctgcag ctcgtgccgc tgctggtagc tgcgctgttt gaaggcggag tccagacgcc
     1261 agtacagcac acctttgatg aggcgcagct tctcgaggct ctgcgcattc tctgagttgg
     1321 ccggcatcga agctgccacc tcctccagat gcaggatgcg cgcccattga tcgcgctccg
     1381 gcggcgtccc gagcgcggcg acgtcgttgc cgatctcgat cgcgttcagc cgcgagtccg
     1441 ccgcaccgcg ctcaccgctc aagcgggccg gcgcgttgct cgccagaagc gcatcagccc
     1501 gtggaagtaa ccctgcgtac gcgcgctcgc gcgtatcgat catcgcgcca aacgcgtcca
     1561 tgctgtcatc ccagcgcagc aggctgcgtt gcagatagcc cagatcgcgc cagttcttca
     1621 ggccctcctg aaaatcgttg tcggccagca gtgcatacag atagcgcgac tgaggagagt
     1681 tgggcagcgt tcgcagttga tagaaccagc cgtaggtgtt ggcattgtcc ttgtcggagg
     1741 ccagcaaatc gtcgagcaga tgtccgccgc gaatatgggc gatcgcgttg tccaggctat
     1801 cggtttcgct ggagaacgac ttgagcgcat cctcataggc ttccgctgcc tggccgccgg
     1861 cgccaagctg cccgtaagcg tagggcaccg ccagatacga ctcctgcacg gcggcatcga
     1921 gcaggccgcg tttgtgcagc tccagccagg gcgccagcgc cgcgcgatag tctttgagcg
     1981 cggcgcgcgc ccagccatcg ccgagcagcg cgcggctgga atacggtccc tccagacgca
     2041 cgcgcgccag cgggatcagc gccggcgcgg gctgatcggc ctgcaggtag gcatagccga
     2101 gcgcgaggtt tgccttgtcg cgcagattca gcaactcagg gctataggta ttgagcgtgc
     2161 cgaccgccgt gaggattgga tcggcctcga ccagccgtcc cgcgcgtacc atggcaacgc
     2221 ccaggttgaa gcgcgcatag gccatccagt cagcaggccc cttccagttc ttcagaatgg
     2281 caatggcctc atcgaaacgc tgctgccgca tcagtacatt gacgagcagg tgcacccgct
     2341 cggcttcggg ttgcggctgc agttgaccgt tgatgtggct gagcgcctgc tcactgcgat
     2401 cgtagtagcc gcgctcgtac cagatcttcg ccagatagaa ccacgcttga ttgcggaccg
     2461 ccggcggcag gcgttcgccg agcatcgttt caaaccggcg accggcctca ttgtgcatgc
     2521 ccagctgcag atacagaccg gccgctagaa gctgcgcgtc cgcatcgtgg tgcggcatcc
     2581 gattccactg ggaatacgct tcgagcagcg tcagcgtctg caggcccgag tcctcgtcgg
     2641 cccagtcatg aaacagcaca tcgccgtagt gcagatcctg caccttgatg ctgggcagct
     2701 tgttgggatc gggtctgtgc ttggcccacg ccacgggagt ggctgcaacc agcagcgatg
     2761 cgcaagccag actcagggat ctgcgccgga atctcatgcg ggaactattc ccagtccttg
     2821 atctcgaatt ccggctcctg agcgtgcgcg tgatcggtaa ccttgagttc cagatacttc
     2881 gcacctacgc ccttctcgaa cttaaggttg gctccccgct tgtagttgcg atcgtgtgga
     2941 cccttgccgt tgaagaacgc caccagttca tgttcgcccg ccttgagatt gccgacgtac
     3001 agccgctgca ccccgccttt gagcagtgcg gccgtctcgc gcggagtgta gaggtagttc
     3061 atcacgtcgc ggttgtctat cttcagcgtg atcgagtcca acgcgaaaaa atcaccgaca
     3121 tcgaccgata cgaacaccgc gacctgcgta ttggccggga acagcagctc ctcctccagt
     3181 acgaacagat ccttattcag atcgaccacg tccttcttca ggacttgaat ctgctgatcg
     3241 agtgcgcgcg tgctgtccgc actcggggcg gtgtcggaat cggacgttga agggggtgcc
     3301 accgcggcat cgggcgaggc agccggcggt gtggtggcgg gcggagtgct tgctggtggc
     3361 tgctgggcgt acaccatacc tgccgacagg caggcggcgg cggtcaggag ccgaagcgtc
     3421 gtgttcataa attttctatc gacaaagtga aggttcatgg taccgccggc ttgttgtcca
     3481 acaatgtgcg tatctgcaca acataggaac gttcgtcggc gcc
//
LOCUS       DATMRM010000244         3569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_128351, whole genome shotgun sequence.
ACCESSION   DATMRM010000244 DATMRM010000000
VERSION     DATMRM010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128351"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VNZ06_07100"
     CDS             complement(<1..1026)
                     /locus_tag="VNZ06_07100"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HWW20555.1"
                     /translation="MSLSARVARQCHRARPRPRLLAAVAGLGLAAALAYSRTWADDAS
                     TVDVDLDGGTLQEVTVTGERQGLLGNVLSASEGIVSDAELQLTPQYRPGQTLETVPGL
                     IVTLHSGEGKANQYLLRGYNLDHGTDLETYVDGMPINQPTHAHGQGYTDLNFVIPELE
                     NGLRYTKGPYYADVGDFGAVGSVRVNYRDTIEDQVSVTAGSFDFQRVLLTGSQALGSG
                     NLLAALEAQRYDGPFSNPDDAHKDNTVLRYSRGDQHSGYCVTGLFYHQLWNNTTDLPE
                     RALAQGLVANRFGTLDPTDGGRAQRASLSAQYHAELGAAKLTASWFYEYNQLHIYNDF
                     THFLNDPV"
     gene            complement(1140..1829)
                     /locus_tag="VNZ06_07105"
     CDS             complement(1140..1829)
                     /locus_tag="VNZ06_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWW20556.1"
                     /translation="MYVLYIANKNYSSWSLRPWILMRELGIAFEERLVPFEPTPVQKA
                     FRAFSPTGKVPCLIDGETRVWDSLAITEYLAERQLDVWPANPDARAWARCAVAEMHSG
                     FTALRVTCSMTCGVRIRLNQVSEALANDLERLEELWTEGLERFGGPFLAGKHFTAVDA
                     FFAPIAFRVQSYSLKLFTRSAQYVARMLEVAAMRTWYAEALAETWRDPPHDEEILKYG
                     KVLKDLRASAA"
     gene            complement(1843..>3569)
                     /locus_tag="VNZ06_07110"
     CDS             complement(1843..>3569)
                     /locus_tag="VNZ06_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWW20557.1"
                     /translation="SLQYRERIADQIALSAGTLDFQRVLGTASQALGEGHLLEALELQ
                     HYDGPFDNPDAARKENAVLRYAQGDQHDGYSLTGMFYHQLWNNTTDIPLRAIAAGFVS
                     NRFGTLDPSDGGRAQRASLSAQFHKSLGDGQLDANAFFIYNQLHLYNDFTHFLVDPAH
                     GDQEVQFENRRVLGGAASYHLPLPIGAIENELLAGVSTRYDLSHLGRTPTQARVPLPS
                     GDDPPSFSNHDGIYLYSLAGYLQATTHWTTSLRSVLGLREDYQHGTDVDYLTALHETD
                     GYNNGGTAHKALLQPKGSLIYTPSEHLEFYLSAGVSFHSTDLRGVNQDKSVDLGLPNA
                     ALLSKQNGEEVGMRVQNGQNFTATLAIYNLWQQSETVLDPDAGVDSAGPPSRRYGAEL
                     NVSYQFTPWLELYASITGNHTRFTQPFDDGTGHVGTYISNAPVAAGSLALYLTDLGRW
                     SGGLEYRYLGSYPLSSGPCSNAAAARDFHDAAASCATAPTATGQINGQGFGELNLDAH
                     YALGTGWSASLGIYNLLNTHAPAAEFWYVDRLRSEIETAPDGVADVHEHPLEPIMWRL
                     TIAKRFGD"
BASE COUNT          656 a         1102 c         1126 g          685 t
ORIGIN      
        1 caccggatcg ttgagaaagt gtgtgaagtc gttgtagatg tgcagctggt tatattcgta
       61 gaaccagctg gcagtcagtt ttgccgcgcc cagttcggcg tggtactgcg ctgacaggct
      121 ggcgcgctgc gcgcgacccc catcggtcgg atccagcgtt ccgaagcgat tggccaccag
      181 accctgcgct agcgcgcgct ccggaaggtc ggtggtgttg ttccagagct gatgatagaa
      241 caagccggtg acgcagtagc cgctgtgctg atcgccccgg ctgtagcgaa gcacggtatt
      301 gtccttgtgc gcatcgtccg gattggaaaa cggaccgtcg tagcgctgcg cttcgagggc
      361 ggccagcaga ttgccgctgc cgagcgcctg cgagcccgtc agcaagacgc gctggaaatc
      421 gaaactaccg gccgtcaccg atacctgatc ctcgatcgtg tcgcgatagt tcacgcgcac
      481 cgagccgacc gcgccgaagt cgccgacgtc ggcgtagtag ggacccttgg tatagcgaag
      541 gccgttctcc agctcaggga tcacaaagtt cagatccgta tagccctgcc cgtgagcatg
      601 cgtcggctga ttgatcggca tgccatcaac gtaggtttcc agatcggtgc cgtgatcgag
      661 gttatagccg cgcagcaagt actggttggc cttaccctca ccactgtgca gcgtcacgat
      721 caggccggga acggtctcaa gcgtctgccc gggccggtac tgcggtgtga gctgcaactc
      781 ggcatcgctg acgatgcctt cgctcgcact gagcacgtta cccaacagcc cctggcgctc
      841 gcccgttacc gtcacctcct gcagcgtgcc accatccaga tccacatcca cagtcgaggc
      901 gtcatcggcc caggttcgcg aataagccag cgcagcggcc aaccccaagc cagcgaccgc
      961 ggcaagcagc ctcgggcgcg ggcgggcgcg atggcattga cgcgcaacac gggcgctgag
     1021 cgacatcgag cctccccacc gcggtgtatg acgaacgatt cattcgtaat acgcccggga
     1081 cgggggtctg gatgccccga cgtcggcgcc gcgccatgtc aactcgaggg cggtgaagtt
     1141 caggcggctg atgcgcgtag atccttcaat accttcccgt atttgaggat ctcctcgtcg
     1201 tgcggcggat cgcgccaggt ttccgccagc gcctcggcgt accaggtgcg catcgctgcg
     1261 acctcgagca tgcgtgcgac atactgcgcg ctgcgcgtga acagcttcag cgagtagctt
     1321 tgaacccgga aggcgatggg tgcgaagaag gcgtccacgg cagtgaaatg cttgcctgcg
     1381 aggaacggcc cgccgaagcg ctccagacct tcggtccata gctcctcgag tcgctccaga
     1441 tcattcgcga gtgcttccga cacctgattc agacggatgc gcacaccgca ggtcatcgaa
     1501 caggtaaccc tcagggcagt aaaccccgag tgcatctccg cgacggcgca gcgcgcccag
     1561 gcgcgcgcat ccggattcgc cggccagaca tccaactggc gttcggccag gtattcggtg
     1621 atcgcgagcg aatcccacac gcgcgtctcg ccatcgatca ggcacgggac tttgccggtg
     1681 ggcgaaaagg cgcggaatgc tttctgtacc ggggtcggct cgaagggaac cagccgctcc
     1741 tcgaacgcga tacccagttc gcgcatcagg atccaggggc gaagcgacca cgatgaatag
     1801 tttttgttgg cgatatacag gacgtacatg agcgtgcatc tcctagtccc cgaacctctt
     1861 tgcgatcgtc agacgccaca tgattggctc gagcggatgc tcgtgcacgt cggcgacgcc
     1921 gtccggagct gtttcgattt cggatcttaa tcgatccacg taccagaact cagccgccgg
     1981 tgcatgcgta ttgagaagat tgtagatgcc gagcgatgca ctccagccag tgcccaaggc
     2041 gtagtgcgca tccagattga gttcgccgaa tccctggccg ttgatctgcc ccgtcgcggt
     2101 gggcgccgtg gcgcaggaag cggcagcgtc gtgaaagtcc cgggcggccg ctgcgttgct
     2161 gcacggcccg gaggacagcg ggtagctacc cagatagcga tattcgagac cgccgctcca
     2221 ccggcctaga tccgtcagat acagcgccag ggagcccgcg gcaaccggcg cgttggaaat
     2281 ataagtgccg acatgcccgg tgccgtcgtc gaatggctgt gtaaagcggg tgtgattgcc
     2341 cgtgatgctg gcgtagagct ccagccacgg ggtgaattga tagctgacgt tcagttcagc
     2401 gccgtaccgg cggcttggcg gtccggcgga gtccacgccc gcatccggat cgagcacggt
     2461 ttccgattgc tgccacagat tgtagatcgc cagcgtggcg gtgaagttct ggccgttctg
     2521 cacccgcata ccgacttcct cgccattctg cttcgacagc agcgcagcgt tcggtaagcc
     2581 caggtccaca ctcttgtcct ggttcacgcc ccgcaggtcg gtgctgtgga agctcacccc
     2641 ggcgctcaga tagaactcca ggtgttccga gggcgtgtag atcaggctgc ctttgggctg
     2701 caacagcgcc ttgtgcgccg tgccgccgtt gttatagcca tcggtttcgt gcagtgctgt
     2761 caggtagtca acatcggtcc catgctgata gtcctcgcgc agcccgagca ccgagcgaag
     2821 cgacgttgtc cagtgggtgg tggcctgcag gtagcccgcc agtgaataca gatagatgcc
     2881 gtcgtgattg gaaaaggagg gtggatcatc cccgctcggc aagggtacgc gcgcttgggt
     2941 gggtgtacgt ccaagatggc tgaggtcgta gcgcgtgctc actccggcca gcagctcgtt
     3001 ctcgatcgcc ccgatcggca gcggcaagtg atagctcgca gccccgccca gcacgcgccg
     3061 attttcgaac tgtacctcct gatccccatg cgccgggtcc accaggaagt gcgtgaagtc
     3121 gttgtacagg tgcagctggt tgtagatgaa gaacgcgttg gcatcgagct gcccgtcccc
     3181 aaggctctta tggaattgtg cggaaagact ggcccgctgc gcgcggccgc catcgctggg
     3241 atcgagcgtg ccaaatcgat tggagacgaa ccccgcggcg atcgctcgca gtggtatgtc
     3301 ggtcgtgttg ttccacaact ggtgatagaa catcccggtc agtgaatagc catcgtgctg
     3361 atcgccctgt gcatagcgca gcaccgcgtt ctccttgcgt gccgcgtccg gattgtcgaa
     3421 cgggccgtcg tagtgttgca gttccagtgc ctcgagcagg tgaccctctc ccagggcctg
     3481 tgaggcggtg cccagcacgc gctgaaaatc gagcgttccg gcgctaaggg caatctgatc
     3541 ggcgatccgc tcgcggtatt gcaggctca
//
LOCUS       DATMRM010000245         4052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3853527, whole genome shotgun sequence.
ACCESSION   DATMRM010000245 DATMRM010000000
VERSION     DATMRM010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3853527"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VNZ06_07115"
     CDS             <1..1179
                     /locus_tag="VNZ06_07115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HWW20558.1"
                     /translation="PATLMAKPPRPRAHATAPTAKESAAGPAVSGTTETPGVPNPPDV
                     YGRAYLLTDDASGQVLAQHDADTRMEPASLTKLMTCYVVFHALKAGTLKLQEPVTVSE
                     HAWRAEGSRTFVQVGSQVPAEVLIKGMIVQSGNDASIALAERVGGTEPAFVQMMNESA
                     KRLGMNNTHFDDSTGLPSTTHYTTARDLAVLASALIRDFPEYYTWFSIREFVWNNIKQ
                     DNRNGLLERDPSVDGLKTGHTDSAGYCLVTSAKREGMRLIAVVLNSPSIKAREDASAA
                     LLNYGFTFYQSVTVKQHGLVVLKPRVYKAKDQYFGIGTATDVVMTVPRAQAASIETSA
                     TAQRRLIAPLSTTTSVGELQVVVNGKPVKSVPLFPLADVPAGGIWSRLSDTVVLWFQK
                     "
     gene            1189..2046
                     /locus_tag="VNZ06_07120"
     CDS             1189..2046
                     /locus_tag="VNZ06_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HWW20559.1"
                     /translation="MASPLPTCYLNGQLMPLQDARISPLDRSFLFGDGVYEVIAVRHG
                     QARRLAANLSRLSRSLKELRIPDPHTSARWTDIIARLVAANGGGDLYVYLQVSRGAEF
                     GRNHAPLPAIEPTVFAFCAPLLLPGEDSLDSGVACITAIDTRWARCDIKSVALLANVL
                     LRQQAVEAGASEAILLRDGMLTEASASSVYVIAGGRICTPPNSYQLLPGTTRTLIEEL
                     ADEVGIPRHVGGITEAELRCADEVWLSAATRGVVAVTSIDGHPIGDGKPGRLWRRIYQ
                     LIEAYWATP"
     gene            complement(2065..3390)
                     /locus_tag="VNZ06_07125"
     CDS             complement(2065..3390)
                     /locus_tag="VNZ06_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWW20560.1"
                     /translation="MKANSPAAALQSWWMPFTHNRHFKAHPRLIESADGAYYTLTDGR
                     RVFDCLSGLWCTPLGHGHPKIVRAVQQQVQRLDFSPGFQFGHTAAFELAARIAALSGR
                     ANDRVFFVNSGSEAVDTSLKIAIAYHRARGEASRTRVIGRERAYHGVGLGGISVGGIP
                     ANRKMFAPLMMPGVDHLSHTYNAAAMRFSRGQPQWGVHLADELERLVELHDASTIAAV
                     IVEPMQGSAGVFVPPRGYLERLRELCTRHGILLIFDEVITGFGRLGTPFASQYFGISP
                     DLTTFAKAVTNGVVPMGGVLARSEIYETFMTGPEHLIEFFHGYTYSGHPLAVAAGHAA
                     LDGLLEEALIERSAQLAKVLESTVHTLRDEPHVADIRNFGLAAAVELQPIAGQAGVRG
                     IRVLERGLDEGILLRLTGDTIALAPPFIASTEEVQGMIESVRRTLRAVA"
     gene            3737..>4052
                     /locus_tag="VNZ06_07130"
     CDS             3737..>4052
                     /locus_tag="VNZ06_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWW20561.1"
                     /translation="MSGSLARVAIVGATGAVGTELIACLQQRQFPLSDLRLFASQRSS
                     GRRMAFCNELLPVEPLEEGSLSSTHIALFSAGAARSRQFAPYAVRAGAIVIDNSSAFR
                     MDA"
BASE COUNT          768 a         1335 c         1217 g          732 t
ORIGIN      
        1 ccggcaacac tgatggccaa accgccgcgc cccagagcgc atgccacggc ccccactgcc
       61 aaggagtcgg ccgcgggtcc tgcggtttca ggcactaccg aaacaccggg agtacccaat
      121 ccgcctgacg tctatggccg cgcttacctc ctcaccgacg acgccagcgg ccaagtgctc
      181 gcgcagcatg atgccgacac gcgcatggag ccggccagtc tcaccaaact catgacttgc
      241 tacgtggtgt ttcacgcgtt gaaggcgggc acgctcaaat tgcaggagcc ggtgactgtc
      301 agcgagcacg cctggcgagc ggaaggctcg cgcacgttcg ttcaggtagg cagccaggtc
      361 ccggccgaag tgctcatcaa aggcatgatc gtgcagtcag ggaacgatgc cagcatcgcg
      421 ctcgccgaac gcgtcggcgg taccgagccg gcctttgttc aaatgatgaa cgaatccgcc
      481 aaacgcctgg ggatgaacaa cacgcatttc gacgacagca ccggcctgcc gtctaccact
      541 cactacacga cggcgcgcga cctggccgtg cttgcgagcg cgctgatccg tgactttccc
      601 gagtactaca cctggttttc gatccgcgaa ttcgtctgga acaacatcaa gcaggacaac
      661 cgcaacggcc tgctcgagcg cgatccttca gtcgatgggc tcaagaccgg acacaccgac
      721 agcgccggct actgtctggt cacatccgcg aaacgcgaag gcatgcgcct gatcgccgtg
      781 gtactcaact cacccagtat caaggctcgc gaagacgcga gcgcggccct gctcaactac
      841 ggatttacgt tctatcagtc cgtcaccgtc aagcagcacg gtctggtcgt actcaagccg
      901 cgtgtgtaca aagccaagga ccagtatttc ggcatcggca ctgccaccga cgtcgtcatg
      961 acggttccgc gcgcccaggc cgccagcatc gagaccagtg ctacggcaca gcgacgattg
     1021 atcgcacccc tgagcacgac gacatcggtc ggcgaactgc aagtggtggt gaacggcaaa
     1081 cccgtcaagt ccgtgccctt attcccgctg gccgacgtgc cggcaggcgg catctggagc
     1141 cggctgagcg atactgtggt gctctggttc caaaagtagc gccgcctcgt ggccagtccg
     1201 cttccgacct gctatctgaa cggccagctc atgccgctgc aggacgcgcg catctcaccg
     1261 ctggatcgca gtttcctgtt cggtgatggt gtctatgaag tcatcgcggt tcgacacgga
     1321 caggcgcgcc ggctcgccgc caatctctcg cgtttgtcgc gcagtctgaa ggagttgcgc
     1381 atccccgatc cgcatacgtc tgcgcgctgg actgacatca tcgcccgcct ggtcgccgcg
     1441 aacggcggcg gcgacctcta tgtctatctt caggtctcgc gtggcgcaga gttcgggcgc
     1501 aatcatgcgc cgctgccggc gattgagcct accgtgtttg cattctgtgc gccgctgctg
     1561 ctccccggcg aggactcgct cgatagcggt gttgcctgca ttaccgccat cgacacgcgt
     1621 tgggctcgct gcgacatcaa gtcggtcgcg ctgttggcga atgtgctact gcgccaacaa
     1681 gcggtcgagg caggcgccag cgaagcgata ctgctgcgcg atggcatgct gaccgaagcc
     1741 agcgcgtcca gcgtgtacgt catcgccggc gggcggatct gtacaccacc gaattcttac
     1801 cagttgttgc caggaaccac acgcacactg atcgaggaac tcgccgacga ggtcggcatt
     1861 cctcgacacg tcggtgggat cactgaagcc gaactgcgct gcgctgatga agtctggctc
     1921 agcgcagcga cccggggagt cgtggcggtc acctcgatcg acggacaccc aatcggtgac
     1981 ggcaagccgg gacggctttg gcgccggata taccagttga tcgaagcgta ctgggctacg
     2041 ccctaacgtc cggatccgcg acgctcatgc cactgcacgc agcgtgcgtc gcacagactc
     2101 gatcatccct tgaacctcct cggtcgaggc aatgaagggc ggcgcgagcg caatcgtatc
     2161 gccggtcagg cgcagcagaa ttccttcatc aaggccgcgt tccagcactc gtattccgcg
     2221 cacgcccgcc tggcctgcga tcggctgcag ttcgaccgcg gcggcgaggc cgaagttgcg
     2281 gatatccgcg acgtgtggct catcacgaag ggtgtgcacg gtgctctcca gcaccttcgc
     2341 cagctgcgcc gaccgctcga tcagtgcttc ttccagcagc ccgtccagcg cggcatgacc
     2401 ggcagcgacc gccagtgggt gaccggaata ggtatagcca tgaaagaact cgatcagatg
     2461 ttccggccct gtcatgaacg tctcgtagat ctcgctgcgc gcaagcacac cgcccatcgg
     2521 caccacgccg ttggtgaccg ctttggcgaa ggtcgtcaga tccggcgata tgccgaagta
     2581 ttgcgatgcg aacggggtcc ccagacggcc gaagcccgta atgacctcat cgaagatgag
     2641 cagaatgccg tgccgcgtgc acagctcgcg caaacgctcc aggtagccgc gcggcggaac
     2701 aaagacaccg gctgaacctt gcatgggctc gacgatcacc gcggcaatgg tcgaggcatc
     2761 gtgcaattcc accagccgct ccaactcgtc cgccagatgc actccccatt gcggctgacc
     2821 gcgactgaat cgcatcgcgg cggcgttata ggtgtgcgat agatgatcga cgccgggcat
     2881 catcagcggc gcgaacatct tccggttcgc aggaatacca ccgaccgaaa tgcctcccag
     2941 acccactccg tgatacgcgc gctcgcgtcc gatcacccgc gtgcgcgatg cttcgccgcg
     3001 cgcgcggtga taggcgatcg caatcttgag agacgtatcg accgcttccg aaccggagtt
     3061 gacgaagaac acacgatcgt tcgctcgccc cgaaagcgcg gcgatgcgcg ccgccagctc
     3121 gaacgcggcg gtgtgaccga actgaaaccc agggctgaag tccaatcgct gcacttgctg
     3181 ctgaaccgct cgcacgattt ttggatgtcc gtgcccaagc ggagtgcacc agaggccgga
     3241 caggcagtcg aacacgcggc gtccgtcagt cagcgtgtag taggcgccgt cggcgctctc
     3301 aatcagccgt ggatgcgcct tgaaatgacg gttgtgcgtg aacggcatcc accacgactg
     3361 cagtgcggcc gcggggctgt ttgctttcat aggtcctgcg ctcgcctgtg acgaagtcat
     3421 gtttgccccg cattggactg gcgataggtt gcccgccgcc gcagactaag ttaccacgag
     3481 cacggcgcgg aatctttggg cggagaccgg gcgtgggcga tgctttacca cccccgccac
     3541 ttcgcctatg cttaggtccc atgctcgtca cgccgaccac gatcaaaacc aaaaccggct
     3601 ccgtcggggc ggtccggtcg tgttcttgtt gacgatagca acatagcatc accgaaggcc
     3661 ccgccggcaa aggacggggc cttttcgttt ccgaggctcc gctccctccg gcctcaccat
     3721 cgaggaacaa cgagtcatgt ctggatctct tgcgcgtgtc gcgatcgtcg gagccaccgg
     3781 cgctgtcggc acggaactca tcgcctgtct gcagcaacgg cagtttcccc tgagcgacct
     3841 gcgattgttt gcctcgcagc gatcgagcgg acggcggatg gccttctgca acgaattgct
     3901 tccggtggag ccgctcgagg aaggctcgtt gtcatcgacc cacatcgcgc tgttctccgc
     3961 aggagccgca cgctcgcgtc agtttgcccc gtacgcggtg cgtgccggcg caattgtgat
     4021 cgacaactcg tcggcatttc gcatggatgc cg
//
LOCUS       DATMRM010000246         3700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2619676, whole genome shotgun sequence.
ACCESSION   DATMRM010000246 DATMRM010000000
VERSION     DATMRM010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2619676"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VNZ06_07135"
     CDS             <1..375
                     /locus_tag="VNZ06_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20562.1"
                     /translation="IAEVLVQDGFFEGKQRVPKGWVARMRQPIAADGRRGFGIWLAAS
                     AHGAESFAADDVFFLRGPGHWRLWLVPGLKLAVLFGADADDEDTKAWDETRVLNLVLR
                     AVSDPVNPADPASKLKGLVPGH"
     gene            400..930
                     /locus_tag="VNZ06_07140"
     CDS             400..930
                     /locus_tag="VNZ06_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWW20563.1"
                     /translation="MSAGIYVLGERRLVTADGDFYIAPGAQVIGSVILGTGASVWFNA
                     VLRADDERIEVGAGSNIQDGCVVHCDVGAPTILGRDVTVGHMVMLHGCVIGDETLIGN
                     GAIVLDRVHIGRHCVIGAGSLLTPDKQFPDNSVVMGAPAKVVRQVSERDLAMIAQGAE
                     HYKARQKLYRQQLRSQ"
     gene            complement(959..1945)
                     /locus_tag="VNZ06_07145"
     CDS             complement(959..1945)
                     /locus_tag="VNZ06_07145"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HWW20564.1"
                     /translation="MEIHKRDEWELPETLRPQQSRFSFDLQTVYRSAVLVHTEASEDG
                     YTAQDLGTERLGSGVVIRSAERKLVLTIGYLITEAESIWLTTLEGKVVEACPLAHDQV
                     SGFGLIQPLGPLDAPPLERGSATSLAIGDKVIVVGHGGERHCLEAQLIARREFAGYWE
                     YLLDEALFTAPPHPQWGGTALVGADGLLLGIGSLFVQESQANESFDANMFVPIDLLEP
                     ILEDMIALGRPRRHPRPWLGLYAAEQADHVIIAGVTRGGPSDKAGVRTDDVILEVASR
                     RVRTLPELFRTIWTSGEAGIQVPLKLSRGEETLHIVVNSATRDDFLKKPARH"
     gene            complement(1955..2875)
                     /locus_tag="VNZ06_07150"
     CDS             complement(1955..2875)
                     /locus_tag="VNZ06_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HWW20565.1"
                     /translation="MGHNVRLFQVDAFTSQRFCGNPAGVVLDADALSSEDMQLIAREL
                     NNGDTAFVLRPDADDHDLRVRFFTPRREAAFVGHATLAVHAVRASLQLAPCARQKQQS
                     GLVDVDTISGASPSRIAIHQSPPTFKRTPAASELSAVLEALTLTQADLDPRCPPTIAG
                     ESSTRLLIGLAHGATLARLQPDLSRLTTLSSQLAAAGYFLFSLQPALADVFSEARMFC
                     PALGIPEDPVSGNAHAMLGAYLLRHSLVQAPTEPTSGEDMVEFTGAQGHHVGRPGRVT
                     VALNLRAGCLDSVTIIGEAVIVFSASLQFE"
     regulatory      2958..3066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3054..>3700
                     /locus_tag="VNZ06_07155"
     CDS             3054..>3700
                     /locus_tag="VNZ06_07155"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWW20566.1"
                     /translation="MRFKSIRAWPLAAVGLSVPALCPAQSSQPAPPSLQEIVITASLR
                     DTYLSDLAQSVTVLDDSTLRAAGVQQLQDVLGEIPNLSWASGSSRPRFFLLRGIGEVE
                     QYQGAPNPSVGFLIDDIDFSGIGMPATLFDTRQVEVLRGPQGTVYGANALAGLISVYT
                     EDPTPDLTLNGEATVGDFSTRAAGVAVSDSVDDARAGWRIAAQQYRSSGYFYNAY"
BASE COUNT          656 a         1118 c         1229 g          697 t
ORIGIN      
        1 attgccgagg tgctggtcca ggatggattc ttcgaaggca agcaacgggt gcctaaaggc
       61 tgggtcgctc gcatgcgcca accgatagcc gccgatggtc ggcgcgggtt tggtatctgg
      121 ctggcggcgt cagcgcatgg cgccgagagt tttgccgccg acgatgtatt ttttctgcgc
      181 gggcccggtc actggcgcct gtggctggtt ccaggtctga agctagccgt gctgttcggc
      241 gctgatgccg acgatgagga cacgaaggcc tgggatgaga cgcgcgtgct gaatctggtc
      301 ttgcgagcgg tgtccgatcc ggtgaatccc gcggatcctg cctccaaact gaaagggttg
      361 gttcccgggc actgagccgg aagtcatcgg gaggatgcga tgagtgctgg gatctatgtc
      421 ttgggggagc ggcgattggt gaccgctgac ggtgacttct acattgcccc cggggcacag
      481 gtgatcggtt ccgtgatatt gggaaccggc gccagtgtgt ggttcaacgc ggtattgcgc
      541 gccgacgatg aacgcatcga ggtcggtgcg ggcagcaaca tccaggacgg gtgtgtcgtt
      601 cattgcgacg tcggcgcacc gaccatcctt ggccgcgacg tcacggtcgg gcacatggtg
      661 atgctgcacg gctgtgtgat cggcgatgaa acactgatcg gcaacggcgc gatcgtgctc
      721 gatcgggtgc acatcggtcg tcattgcgtc atcggtgcag gctccctgct gacgccggac
      781 aagcaatttc cagacaattc ggtggtgatg ggcgcgcctg ccaaggtcgt gcggcaggtc
      841 agcgaacgcg atctggcgat gatcgcgcag ggcgccgagc actataaggc ccggcagaag
      901 ctctaccgcc agcagttgcg atcgcaatga aggcgatgcg tcccgatcct tgccgctgtc
      961 agtgcctcgc gggcttcttg agaaaatcgt cgcgtgtcgc ggaattcacg acgatatgca
     1021 gcgtctcctc gccgcgcgag agtttcagcg gcacctggat cccggcttcg cccgaggtcc
     1081 agatggtgcg gaataactcc ggcagggtgc gtacacgacg cgaggccacc tccagaatga
     1141 catcatccgt gcgcaccccg gccttgtcgg atgggccgcc gcgggtgaca ccggcaatga
     1201 tcacgtgatc ggcttgctcc gcggcataca gtccgagcca cggacgcgga tgtcgccgcg
     1261 ggcggcccag cgcgatcatg tcctcgagta tcggctcgag cagatcgatc ggtacgaaca
     1321 tattggcgtc gaaactctcg ttcgcctgcg actcctgcac gaagagcgag ccgatgccga
     1381 gcagcagacc gtcagcaccg accagcgcgg tgccgcccca ctgagggtgc gggggtgcgg
     1441 tgaatagggc ctcatcgagc agatactccc agtagccggc gaattcacgc cgcgcgatca
     1501 actgggcttc gagacaatgg cgctcgccgc catgcccgac cacgatcact ttgtcgccga
     1561 tcgcaagtga ggtggccgac ccgcgctcca gcgggggtgc gtccagcggg ccgagcggct
     1621 gtatgagccc gaagccactg acctgatcgt gggccaacgg acaggcttcg acgactttgc
     1681 cctcgagggt cgtcagccag atggattcgg cttcggtgat cagatacccg atcgtcagta
     1741 ccagcttgcg ctcggcactg cggatgacga cgccggagcc gaggcgctcg gtccccagat
     1801 cctgcgcggt gtagccgtct tcgctggctt ccgtgtgaac caacaccgct gaacggtaga
     1861 ccgtctgcag gtcgaagctg aagcgactct gttggggacg caacgtctcc ggcaactccc
     1921 attcatcccg cttgtggatc tccatgcggc gatgttactc gaactgcagc gacgcgctga
     1981 agacgatcac agcctcgccg atgatggtta ccgaatcgag acatcctgct cgcagattca
     2041 gggcgaccgt gacccgacct ggtctgccga cgtggtgtcc ctgagcgccg gtgaattcaa
     2101 ccatgtcttc gccggaggtc ggttcggtcg gtgcctggac cagcgaatgc cgcagcagat
     2161 aggcacccag catggcgtgc gcgttgccgc tgaccggatc ctccgggatt ccaagcgcgg
     2221 ggcagaacat acgcgcttcg ctgaacacgt ccgccagcgc gggttgcagc gagaacagaa
     2281 agtagcccgc cgccgcgagc tgcgaggaca gcgtcgtcag gcgcgacaga tccggctgaa
     2341 gtcgggcaag ggtcgcgccg tgggccaacc cgatcagcag ccgcgtactg ctttcgcccg
     2401 cgatcgtggg cggacagcgc ggatcaagat ctgcctgcgt cagtgtcagt gcctctagca
     2461 ccgcggacaa ttcgctcgca gcgggtgtgc gcttgaatgt cggcggcgac tggtgaatcg
     2521 caatgcgcga cggcgatgcg ccgctgatgg tgtccacatc gaccagaccg ctctgctgct
     2581 tctgccgtgc gcatggcgca agttgcagcg acgcccgcac cgcgtgcacc gcaagtgttg
     2641 catggccgac gaacgcggcc tcgcgccgcg gtgtgaagaa ccgtacgcgc agatcgtgat
     2701 cgtccgcatc cgggcgcagc acgaacgcgg tgtcgccgtt gttgagctcg cgcgcgatga
     2761 gctgcatgtc ctcgctcgag agcgcgtccg cgtcgagcac cactcctgcc ggattgccgc
     2821 agaagcgttg cgacgtgaat gcatcgacct gaaacagtcg cacgttgtga cccatgattg
     2881 gctccgccag taattcccgg cgattgtagc gagcttgaac ggcttgcatt cgagccttgt
     2941 ttgatcgata gttgatcgcg aacaccttgg ggtggtcctg agcggacctg agatgccgat
     3001 ggtcggcgaa cccgttgaac ctgatccggt tagtaccggc gtagggagca ggtatgagat
     3061 tcaagtcaat tcgcgcgtgg ccactggcgg ccgtcggctt aagtgtgccc gcactttgtc
     3121 cagcgcagtc atcgcagcca gcgccgccat cgctgcagga aatcgtgatc accgccagcc
     3181 tgcgcgatac ctatctgtcg gatctggcgc agagtgtgac ggtgctggat gacagtacct
     3241 tgcgcgctgc cggagtccag cagctgcagg atgtgctggg cgaaataccg aacctgagct
     3301 gggcgagcgg cagttcgcgg ccgcgttttt tcctgctgcg tggtattggt gaagtcgagc
     3361 agtatcaggg agcgccgaac ccctcggtcg gttttctgat cgacgacatc gatttctccg
     3421 gcattggcat gccggcgacg ctgttcgaca cccgccaggt cgaagtgctg cgcggtccgc
     3481 agggtacggt gtatggtgcg aacgcgctcg ccggcctgat cagcgtatac accgaggatc
     3541 caaccccgga tctcacgctg aatggtgagg cgaccgtggg cgacttttca acccgcgcgg
     3601 cgggcgtcgc cgtcagcgac agtgtcgatg acgcgcgggc cggctggcgc atcgccgcgc
     3661 agcaatatcg cagcagcggc tatttctaca atgcttacta
//
LOCUS       DATMRM010000247         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_764781, whole genome shotgun sequence.
ACCESSION   DATMRM010000247 DATMRM010000000
VERSION     DATMRM010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764781"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VNZ06_07160"
     CDS             complement(<1..485)
                     /locus_tag="VNZ06_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HWW20567.1"
                     /translation="MKKASASKTQELLKRRQAPLETPTDLKASATRDITASMNAILAD
                     VFALYLKTKNFHWHMSGPHFRDYHLLLDEQADQIFAMTDPIAERVRKLGGATIKSIGH
                     IARSQRILDNDASYVDPLDMLAELCEDNKTLAARLREVHNVCDEYRDIATASLIEVWI
                     D"
     gene            complement(526..2178)
                     /gene="pgm"
                     /locus_tag="VNZ06_07165"
     CDS             complement(526..2178)
                     /gene="pgm"
                     /locus_tag="VNZ06_07165"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HWW20568.1"
                     /translation="MSDRISALAGQPAPRELLINVPRLITAYFSERPDASVAEQRVAF
                     GTSGHRGTSLRQSFNEWHVLAISQAICEYRKGEGIDGPLFMGADTHALSEPALVSALE
                     VLAANGVEVMLSDGDEYTPTPAVSHAILTHNRGRRSKLADGIVITPSHNPPDAGGFKY
                     NPANGGPADSQVTRGIERRANELLLNGLNGLSRVPLQKALHSATTHRYDYLNTYVRDL
                     GNVVDLGAIAAAKVRMGVDPLGGAGAHYWAAIAESYRLDLTVVSDVVDPTFSFMTLDW
                     DGKIRMDPSSGYAMRRLIGLKDRFDVAFACDTDHDRHGIVARSSGLMPSNHYLATSIY
                     YLFQNRPQWRRETAIGKTVVSSQMIDRVAAKLERKLYEVPVGFKYFVDGLLSSALGFT
                     GEESAGATFLRMDGSVWTTDKDGIVAALLSAEITARMKRDPGEVYRGLTREFGECVFD
                     RTDARATPAQKALLSKLSAQQVKSTTLAGEPIKSILTHAPGNGEAIGGLKVIAEHGWF
                     AARPSGTEDIYKVYAESFRGEQHMRRIASEAESIVSAALAAG"
     gene            complement(2175..>2830)
                     /locus_tag="VNZ06_07170"
     CDS             complement(2175..>2830)
                     /locus_tag="VNZ06_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20569.1"
                     /translation="LDVDNTLLDNDAVHRDLSDHLERQFGAASRDRYWAIFETLRGEL
                     GYADYLGALQRYRCEAPNDTRLLLMSGYLMDYPFAGRLYPGALGAIEHLLRLGPTVIL
                     SDGDVVFQPRKVQRSGLWEAVQGRVLIYIHKEEMLADISRLYPAQHYIMVDDKLRILT
                     AMKQQWGSRLTTIFPRQGHYALDPKLVAAFPAADVTVERIGDLAQSDLGQLLAGQRS"
BASE COUNT          512 a          893 c          869 g          556 t
ORIGIN      
        1 tcgtcgatcc agacttcgat taaactggca gtggcgatgt cgcggtattc atcgcaaacg
       61 ttatggacct cacgcagacg tgccgccagg gtcttgttgt cctcgcacag ctcggccaac
      121 atgtcgagcg gatcgacata gctggcatcg ttgtcgagga tgcgctgcga gcgcgcaatg
      181 tggccgatcg acttgatcgt cgcgccgccc agcttgcgca cccgctcggc gatcgggtca
      241 gtcatcgcga agatctgatc ggcctgctca tcgagcagca gatgatagtc gcgaaagtgc
      301 gggccgctca tgtgccagtg gaaattcttg gtcttcagat acagcgcgaa cacgtcggcc
      361 aggatcgcat tcatcgaggc cgtgatgtcc ctggtagcgc tggccttcag atcggtcgga
      421 gtctcgagcg gggcctggcg ccgcttgagc aattcctggg tcttggacgc ggatgctttc
      481 ttcatgggtt ggctgccttg tgttcttcgg aaaaatactg gggagtcagc cggctgcaag
      541 agctgcggaa acgatgcttt cggcctcgct cgcgatgcgc cgcatgtgct gctcgccccg
      601 gaaactctca gcgtagacct tgtagatgtc ttcggtaccg gaaggtcgcg ctgcaaacca
      661 gccgtgttcg gcgatcactt tgagtccacc gatcgcctcg ccgttgccgg gggcgtgagt
      721 gagaatgctc ttgatcggct ctccggccag tgtggtgctc ttcacctgct gcgctgacag
      781 cttcgacagc aaggccttct gcgctggcgt ggcgcgggca tcggtgcggt caaacacgca
      841 ttcgccaaac tctcgcgtca ggccacgata cacctcgcct gggtcgcgtt tcatgcgcgc
      901 ggtgatctcg gccgacaaca gcgcggcaac gatgccatcc ttgtcggtcg tccagacgct
      961 tccgtccata cgcaggaagg tcgcgcccgc actctcctct ccggtaaagc ccagggcgct
     1021 tgaaagcagg ccgtcgacga agtacttgaa gcctaccggc acttcgtaca gtttccgctc
     1081 cagcttcgcg gccacgcgat cgatcatctg gctgctgacc accgtcttgc cgatcgcggt
     1141 ctcgcgccgc cactgcggcc ggttctgaaa cagatagtag atggaggtgg ccagataatg
     1201 attcgacggc atcagtccgc tgctacgcgc gacgatgccg tgccgatcat ggtcggtgtc
     1261 acaggcgaag gccacgtcaa agcgatcctt caatccgatc agccgacgca tcgcgtagcc
     1321 ggacgagggg tccatgcgaa tcttaccgtc ccagtccagc gtcatgaagc tgaaggtcgg
     1381 atcgactaca tcgctaacga ccgtcagatc gagacggtag ctttccgcga tcgcggccca
     1441 gtagtgcgcg ccggcgccgc cgagcggatc cacacccata cgcactttcg ccgcggcaat
     1501 cgcgcccagg tccacgacat tgccgaggtc gcgcacatag gtgttcagat aatcgtatcg
     1561 atgtgtggtg gcgctgtgca gcgctttctg cagcggcacg cgcgagaggc cattcaagcc
     1621 gttcagcagc aactcgttcg cacggcgctc gatgccgcgt gtgacctggc tatcggcggg
     1681 ccccccgttg gcagggttgt atttgaagcc gccggcatcc ggcggattgt gtgacggcgt
     1741 gatgacgatg ccgtccgcaa gcttagagcg gcggccgcga ttgtgcgtca ggatagcatg
     1801 cgagaccgcc ggcgtcggag tgtattcatc gccgtcggac agcatcacct ccacaccgtt
     1861 ggcggccagc acttccagcg cgctcaccaa cgccggctcg gacagggcgt gcgtatcggc
     1921 gcccatgaac agcgggccat cgatcccctc gcctttgcgg tattcgcaga tcgcctgact
     1981 gattgccagc acgtgccatt cgttgaagct ctgccgcaac gaggtcccgc gatgccccga
     2041 tgtgccaaat gcgactctct gctccgccac gctcgcgtcc gggcgctcgg agaaataggc
     2101 ggtgatcagt ctggggacat tgatcagcaa ctcgcgcgga gcgggctgac ccgccagcgc
     2161 actgattcgg tcgctcacga tcgctgaccc gccagcagtt gcccaagatc cgattgcgcc
     2221 agatcgccga tgcgttcgac cgtgacatca gctgccggaa aggctgccac cagcttggga
     2281 tcgagcgcgt agtgcccctg acgcgggaaa atggtcgtca ggcgcgatcc ccactgctgc
     2341 ttcattgccg tcaggatccg cagcttgtca tccaccatga tgtagtgttg cgcaggatag
     2401 agccggctga tgtcagccag catctcttcc ttgtggatgt agatgagtac ccggccctgc
     2461 accgcctccc acaggccgga gcgttgcacc ttgcgcggct ggaacaccac gtcgccgtcc
     2521 gagagaatca ccgtcgggcc caagcgcagc agatgctcga tggcgcccag tgcgcccgga
     2581 tacagccgtc cggcgaacgg atagtccatc agatatcccg acatcagcag caggcgcgta
     2641 tcgttcggtg cctcgcagcg gtagcgctgc agggcaccga gatagtcggc atagccgagt
     2701 tcgccacgca gggtctcaaa gatcgcccag taacgatcgc ggctggcagc gccgaactgt
     2761 ctttccaggt gatcgctcag gtcacggtgc acggcatcat tgtcgagcag cgtgttgtcg
     2821 acatcgagca
//
LOCUS       DATMRM010000248         7783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3853725, whole genome shotgun sequence.
ACCESSION   DATMRM010000248 DATMRM010000000
VERSION     DATMRM010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3853725"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VNZ06_07175"
     CDS             complement(<1..1242)
                     /locus_tag="VNZ06_07175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HWW20570.1"
                     /translation="MHSVADAADRQLNDTDKQFLGHPRGLAFLGFTEGWERFSFYGMQ
                     TLLVLYMAKQLLLPGHVEHIAGFTGVRAALESAYGHPLSVIALGSAIYGVYSGSVWIT
                     PILGGLIADRWLGRTRTITIGAVLMAAGHFLMAFEAPFLLALACLLIGVGCFKGNLAS
                     QVGSLYSAADLRRADAYQIYYVFINAGVIIAPLVAGTLGERYGWHYGFGIAGVGMLIS
                     LAVYRAGRHWLPPDAPVVRSARSAAGSRRLPPGERRAMIALLLLLPALALAAVGNQQI
                     FNAYLLWVPDHADMTFFGQTMPTSWLITVDAGLSLGALGGSLLFWRLWALRFPEPSEV
                     TKIAIGMLISMAGLLMLVVAGSLSAGGQRIRILWPLAFDTLNSIGYANVFPVGLALYA
                     RASPRSLAATMIGIYYGHLFVA"
     gene            complement(1280..2296)
                     /locus_tag="VNZ06_07180"
     CDS             complement(1280..2296)
                     /locus_tag="VNZ06_07180"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWW20571.1"
                     /translation="MQGIARAAFFREPQGVLPTTLVLSWCSVGWLGSFALMASQHLLW
                     NALGVLVCAHGMLIAAYMIHEAAHQSVLATPRANAAVGECLSFIAGASYVSFERIRHM
                     HMRHHRDRVDLACFDHQSVLRAHRWLRRLIQIAEWAYLPATEVLLHVQLVWRPMSQRS
                     QRRYLPRVLAMLLLRGTLWVALALWSLKSALLYLFAYALLLHVLNFFDAFHHTFEQHF
                     VAADQPVPGARPDREYEMAHTYSNLLSVRWPWANTLILNFCYHNAHHERASAPWYRLR
                     VVHRAIYGEQHRAVLPLRELLWTWHRNRVSRVDCEDYGLPGEGSGRADRFIGVHGVSF
                     LTVV"
     gene            complement(2408..2611)
                     /locus_tag="VNZ06_07185"
     CDS             complement(2408..2611)
                     /locus_tag="VNZ06_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20572.1"
                     /translation="MEPNTAAQRSPVVTHLAGYSLLASIKPQPVGRETEYGCVDWYTY
                     ADTAQSRKDWFDHDSGLAVGDAA"
     gene            complement(2735..4036)
                     /locus_tag="VNZ06_07190"
     CDS             complement(2735..4036)
                     /locus_tag="VNZ06_07190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWW20573.1"
                     /translation="MDHDVLILGAGIAGLTAARQLAERGARVCVLEARERVGGRIWTQ
                     HLSSPKEEGSMPVELGAEFVHGLPQEIVRLAHEAGISIDERQGRTMQFIDGRLLRPPS
                     APSGFDVLAQMQQWLEAQPPGSDLSFTQYLERVPPPESQRKGAIAYVEGFNAADSRVI
                     SIASLSAQQRAEDADQGDRIFHVRAGYDALPGFLAQRLARAGGVLRLGSAVRRIDWKP
                     GEVTMRGEDAAGSAFSVSAARAVITLPLGVLQTGAVEFAPVPASALQAARRMAFGPAM
                     RMTLVFRHAFWRDLGPDMQELGFLFANDESPRTWWTPMPDRSPMITAWVGGPSAVLPH
                     DSWLDRCLATLARITGQQKAQLRQWLVSSHCHDWLADPLVRGAYSYVPVGALAASEQM
                     SQPVGETLFFAGEHTDLSFNWGTVHGAIRSGLRAARQLIVM"
     gene            complement(4129..4449)
                     /locus_tag="VNZ06_07195"
     CDS             complement(4129..4449)
                     /locus_tag="VNZ06_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20574.1"
                     /translation="MKTGLIVIFAVVAAYVLTACEQASSPSTVDKRVAEARMDAQKQD
                     SKALAEAAVTQAEGRHDVAIQRCNAFSGDQQKACKAQADAALDLSKANVKAQKVAADN
                     GNSP"
     gene            complement(4564..5673)
                     /locus_tag="VNZ06_07200"
     CDS             complement(4564..5673)
                     /locus_tag="VNZ06_07200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWW20575.1"
                     /translation="MRRRRSDLHLIRKRRDEAPVHWSVPPPPHIRYPTVPEQAMQFLT
                     RYLFAVLGLIFFNFAPASAPVWLTMWHFNLIVAVYLAINTANLIHAQYQPRSEMRYRT
                     ALWIDVVIVTICVANDPNEIPPSLVAYIVVVLGNGMRYGIRFFGEALCATLIGAGAGL
                     AARYWHQHHTISEGTLFLSLFGAIIIIYSYILMGRIERSRRRTEQRMRTDALTGLLNR
                     HGLSEAVENWAADRRAMTRKPVVVLADLDNFKLVNDTRGHAEGDRVLQEVASMLGRCL
                     RGGDLIARYGGDEFVAVLADVEPREAQFIVNRVQTEVENWFRDNQLACGISIGCGTAS
                     SDHWDLDQVLQSADRLLYESKLKRGSLRTHLADRI"
     gene            complement(5688..7100)
                     /locus_tag="VNZ06_07205"
     CDS             complement(5688..7100)
                     /locus_tag="VNZ06_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWW20576.1"
                     /translation="MSETGAGTAPDLPRVLGGTHATAIVVGTIIGSGIFLVPSEMMQD
                     VGSSLLVYAAWIVGGLLSLFGVMTYSELGAMMPYAGGEYVYLRGAYGDRMSFLYMWTW
                     FAVAKPASIGTVTMGLARTLGIFPAFHWLDTAAIGGQFPLMWSQLFAIGVTWLITGLN
                     YLGIKKAGDFQVVFTVLKVLLILVVAGFCFSSGAGSWSNFGTALPHAASGNAFNNFMA
                     AVIATLWAYDGWNDLTMVAGEIRQPQRNLPLALIGGLLIVGLLFMATNAAIQYVLPAA
                     QIAASPRPAVAALQVVAGSAGASLVAAGMALSIFVTLNGTIMSGARVPYAAARDRLFF
                     PRFATIHPRFQSPSTSLLVQGLLASILLLALGRFRQLFELAVFAEWLFYLLTATTLFV
                     YRRRIPQAPRPYRVWGYPLLPAVFVLCSGVVLASSYVGNLAGSLIGTLLILLGLPVFE
                     WIRRQRFILPGVAPQTLTTP"
     gene            complement(7109..>7783)
                     /locus_tag="VNZ06_07210"
     CDS             complement(7109..>7783)
                     /locus_tag="VNZ06_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HWW20577.1"
                     /translation="IPVIAVLFSGRPLIVPWLFERADAILAAWFPGAEAGHAVADVLT
                     GKVSPSARTPVSWPRSMGQVPVFFAQRPSGRPANPTDHYTSKYLDSPNEPVIHFGHGL
                     SYGQFRYSNLRVTPERARESDTLQILVDLVNVGPREGEETVFLFARDRLASVARPVLE
                     LKGFGRILLQPGQSGTVSLQLPAAELGFLGADLTRVFEPGEIEILVGPSADRARLLPH
                     TVSLEA"
BASE COUNT         1577 a         2622 c         2363 g         1221 t
ORIGIN      
        1 cgcgacgaac aggtgcccgt aataaatccc gatcatggtc gccgccagcg acctcggtga
       61 ggcccgcgca tacagcgcca gacccaccgg aaacacattc gcgtagccga tgctgttgag
      121 ggtgtcgaaa gccagcggcc acagaatacg gatccgctgc ccaccggctg acagtgaccc
      181 ggcgaccacc agcatcagca gccccgccat cgagatcagc atgccgatgg cgatcttcgt
      241 cacctcgctg ggttcgggaa agcgcagcgc ccacagtcgc cagaacagca gcgagccgcc
      301 cagtgcaccc agcgacagtc cggcatcgac cgtgatcagc catgaggtcg gcatggtctg
      361 gccgaagaag gtcatgtccg cgtgatccgg cacccacagc aggtaggcat tgaagatctg
      421 ctggttgccg accgccgcca gcgccaatgc cggcagcagc agtagcaacg caatcatcgc
      481 gcgacgctca ccgggtggca gacgccgcga gccggcagca ctgcgcgctg aacgaaccac
      541 gggcgcatcc ggcggcaacc agtgcctgcc tgcgcggtag acagccagcg agatcagcat
      601 gccgacgccg gcgatgccga agccgtagtg ccagccgtag cgctcgccga gcgtaccggc
      661 caccagcggc gcgatgatca cgccggcgtt gatgaaaacg taatagatct gataggcatc
      721 ggcgcgtcgc aggtcagccg ccgagtacag cgagccgacc tgactggcga ggttgccctt
      781 gaagcagccg accccgatca gcagacacgc cagcgccagc aggaacggcg cttcgaacgc
      841 catcaggaaa tggccggccg ccatcagtac cgcgccgatc gtgatggtac gcgtgcggcc
      901 gagccagcgg tcagcaatca ggccaccgag gatcggtgtg atccagaccg acccggaata
      961 cacgccatag atcgccgatc cgagcgcgat gaccgacagc gggtgaccgt atgcggactc
     1021 cagtgccgcg cgcacgccgg taaagcccgc gatgtgctcg acatggccgg gcagcagcag
     1081 ctgcttggcc atgtacagca ccagcagcgt ctgcatacca tagaaggaaa agcgctccca
     1141 cccttcggtg aagccgagga aggccaggcc gcgcggatga ccgaggaact gcttgtctgt
     1201 atcgttcagt tgccggtcag cggcatccgc cactgagtgc atcggcgcat catagccgac
     1261 ctcatccgcg cagcgcccgt cagaccactg tcaggaacga gacgccatgg acaccgatga
     1321 agcggtcggc acgcccgctg ccctcgcccg gcagcccgta gtcctcgcag tccacccgac
     1381 tcacgcgatt gcgatgccag gtccacagca gttcgcgcag tggcagcacc gcgcgatgtt
     1441 gctcgccgta gatggcccgg tgcaccaccc tcaagcgata ccagggtgct gacgcacgct
     1501 cgtggtgcgc attgtgatag cagaaattca ggatcagagt gttcgcccaa ggccagcgca
     1561 ccgacagcag attcgaatag gtatgggcca tttcatattc acggtccgga cgcgctcccg
     1621 gcaccggctg atcggccgcg acaaaatgct gctcgaacgt atgatgaaac gcgtcgaaga
     1681 aattcagcac atgcagcaac agcgcatacg cgaacaggta cagcagcgct gacttcagtg
     1741 accacaacgc cagcgcgacc cacagcgtac ctctgagcaa caacatcgcg agcactcgcg
     1801 gcagatagcg acgctggctg cgttggctca tcggtcgcca gacaagctgc acatgcagca
     1861 gcacttccgt cgccggcagg taagcccact ctgcgatttg gatcaggcgg cgcagccacc
     1921 gatgcgcgcg cagcacgctc tgatggtcga agcaggccag atccacgcga tcgcggtgat
     1981 gacgcatgtg catgtgtcgg atgcgctcga acgagacata gcttgcgccc gcgatgaaac
     2041 tcaagcactc acccaccgct gcattggcgc ggggtgtcgc caacaccgac tgatgcgcgg
     2101 cctcatggat catataggcg gcgatcagca tgccatgcgc gcagaccagc acgcccagcg
     2161 cattccacag caggtgctgc gaagccatga gtgcaaagga ccccagccac ccgacactgc
     2221 accaggagag caccagcgtg gtcggtaaca ccccctgcgg ctcacgaaag aatgccgcgc
     2281 gcgcgatccc ctgcatcgtc cgatcccttg aagttcttgg gtttcctggg acagggtcca
     2341 gcaagcgccg tgccggcgat gcgcaccgct ggagcgaatc agcgttggag aggaacgaag
     2401 gctctaatca ggcagcgtcg ccgacagcca ggccgctgtc gtggtcaaac cagtctttgc
     2461 gtgattgcgc cgtgtcggca taggtatacc agtcgacaca gccgtactcc gtttcgcgcc
     2521 cgaccggttg cggcttgatg gaagccagca atgagtagcc ggcaagatgg gtgacaactg
     2581 gggatcgctg cgccgcggta ttcggttcca tcatcgttgc tccgcgtcaa actagtgatg
     2641 aatgttagct gaagactatg cgctcaatca cactattgcc ccgattaagt tgagcggatg
     2701 tcggagattc tgctacccag ctctaggaat tctcctacat gacgatcagt tgacgcgccg
     2761 cgcgaaggcc actgcggatg gcgccgtgca cggttcccca gttgaagctc aaatccgtgt
     2821 gctcgccggc aaagaacagc gtctccccga ccggctgact catctgctcg gacgctgcca
     2881 gcgcgcccac gggcacatag ctgtaagcgc cccgcaccag tggatcggcc agccagtcgt
     2941 ggcaatgtga gctgaccagc cattgccgaa gttgcgcctt ctgttgcccc gtgatccgcg
     3001 cgagcgtcgc cagacaacga tcgagccacg agtcatgcgg cagcaccgca cttgggccgc
     3061 ctacccaggc ggtgatcatg ggtgaccgat ccggcatcgg tgtccaccag gtgcggggtg
     3121 actcatcgtt tgcaaacaga aagcccagct cctgcatatc cgggcccaga tcgcgccaga
     3181 aggcgtgccg aaataccagc gtcatgcgca tcgccggacc aaacgccatg cgccgtgcag
     3241 cctgcagtgc gctcgcaggc accggcgcaa actcaacggc tccggtctgc agcacaccca
     3301 gcggcagcgt gatcaccgca cgtgcagcgc tcacactgaa agcactgccg gccgcatcct
     3361 cgccccgcat cgtcacctcg cccggcttcc agtcgattcg tcgaaccgcg ctgcccagcc
     3421 gcagcacgcc cccggcgcgc gcgagccgct gcgccagaaa ccccggcagt gcgtcatagc
     3481 cggcgcgcac gtggaaaata cggtcgccct gatcggcgtc ctcggcgcgc tgctgcgcac
     3541 tcagtgaggc gatgctgatc actcgactgt cggctgcgtt gaatccttcg acataggcga
     3601 tcgcgccttt gcgctggctc tcgggtggtg gcacccgctc cagatattgc gtgaaactca
     3661 ggtccgagcc gggaggctgt gcctcgagcc actgctgcat ctgcgccagc acatcgaaac
     3721 cggatggcgc cgacggcggc cgcagcagcc gcccgtcgat gaactgcatc gtgcgacctt
     3781 gtcgctcatc gattgaaatg cccgcctcgt gcgccagtct cacgatctcc tgcggcaggc
     3841 cgtgcacgaa ctcggcgccc agttcgaccg gcatcgaacc ctcctctttc ggtgaggaaa
     3901 ggtgctgcgt ccagatccgc ccgccgacgc gctcccgcgc ctcgagcacg cacacacggg
     3961 cgccgcgctc cgcgagttgc cgcgccgccg tcaaaccggc gatgccggcc cccagaatca
     4021 gaacatcatg gtccatcgga cctttgcgac cgggccacgc ggattgttca gacagcctcc
     4081 cagtgtccgg gtccgactcg tgggaatcaa tagcggccgt caggaccctc agggcgaatt
     4141 gccgttatcg gccgccactt tctgtgcctt gacattcgcc ttcgacagat ccagggccgc
     4201 gtccgcctgc gctttgcagg ccttctgctg atcaccggaa aaggcattgc agcgctgaat
     4261 cgcgacgtca tgccgaccct ccgcctgcgt cacggcggct tcggccagtg ccttgctatc
     4321 ctgcttctgc gcgtccatgc gtgcttcggc gacgcgcttg tccaccgtgg acggcgagga
     4381 tgcctgctca caagccgtca gcacgtacgc ggcaacgacg gcaaaaatca cgatcagacc
     4441 ggtcttcatg acgatagtct ccttgaggtc gaggaccatc agcctaggca cggcgccgct
     4501 gcgcgcggct aggtgcagtg gaaaatcgcc tgtaggacag gccgctttat tcgcatcggc
     4561 ggatcagatc cgatcggcca ggtgcgtcct gagtgagccg cgtttgagct tggattcgta
     4621 cagcagccgg tccgccgact gcaagacctg gtccaggtcc cagtggtcac tcgatgcggt
     4681 cccgcaacca atgctgatac cgcacgcgag ctgattgtcg cgaaaccagt tctcgacctc
     4741 ggtctgcacc cggttcacga tgaactgcgc ctcgcgcggt tccacgtcgg ccagcaccgc
     4801 cacgaattcg tcgccgccgt agcgcgcgat cagatccccg cccctcaggc agcgcccgag
     4861 catcgacgcc acttcctgca gcacccggtc gccttcggca tgcccgcgcg tatcgttgac
     4921 gagcttgaag ttatccaggt ccgccagcac cacgacgggc ttgcgagtca tcgcgcgccg
     4981 atcggcagcc cagttctcca ccgcttcgct caggccatgg cgattgagca gcccggtcag
     5041 cgcatcggtg cgcatgcgct gctcggtgcg tcggcgcgag cgctcgatgc gccccatcag
     5101 aatgtatgaa tagatgatga tgatggcgcc gaacagactc aggaacagcg tgccttcgct
     5161 gatcgtgtgg tgctggtgcc agtagcgcgc ggccaaaccc gccccggcgc cgatcagcgt
     5221 cgcgcacagc gcttcgccaa agaaacggat gccatagcgc atgccattgc cgagtaccac
     5281 cacgatgtag gccacgagcg agggcggaat ctcgttggga tcgttcgcca cacagatcgt
     5341 gacgatcacg acgtcgatcc acagcgccgt gcggtagcgc atctccgagc gcggctggta
     5401 ctgcgcgtga atcagattgg cggtgttgat cgccagatag actgcgacga tcagattgaa
     5461 gtgccacatc gtcagccaca ccggcgccga tgcgggagcg aagttgaaga agatcagccc
     5521 cagcactgcg aacaggtagc gcgtcaggaa ctgcatcgcc tgctcaggca cggtgggata
     5581 tcggatgtgc ggcggcggcg gcacggacca atgcaccggc gcttcatcgc gacgttttcg
     5641 tatcaggtgc agatcgctgc ggcggcggcg catgggttct aaagagctca gggggtcgtt
     5701 aaagtttgtg gggcgacgcc aggcagtata aaccgttggc gccggatcca ttcgaaaaca
     5761 ggcagcccca gcaggatcag caacgttccg atcagtgagc cggcgagatt gccgacgtaa
     5821 ctggacgcca gcaccacgcc cgagcacagc acgaaaaccg ctggcagcag cggatatccc
     5881 catacccggt agggccgcgg cgcctgcgga atacggcgcc ggtaaacgaa cagtgtggtc
     5941 gcggtcagca ggtagaacag ccactccgcg aacaccgcca gctcgaacag ctgccggaag
     6001 cgacccagcg cgagcagcag tatcgaggcc agcaaaccct gcaccagcag cgaggtcgac
     6061 gggctctgaa aacgcggatg aatagtcgcg aatcgcggga aaaacagccg atctcgcgcc
     6121 gccgcatacg gcacgcgcgc accggacatg atggtgccgt tgagcgtgac aaagatgctc
     6181 aatgccatgc ccgccgccac cagcgaggcg ccggccgagc ccgccaccac ttgcagggcc
     6241 gcaaccgccg gccgcggtga ggcggcaatt tgcgccgccg gcagcacata ttgaatggcc
     6301 gcgttggtcg ccatgaacag cagacccacg atcaggaggc cgccgatgag cgccagcggc
     6361 aggttgcgtt gcggctgtct gatctcgccg gcgaccatgg tcagatcgtt ccagccgtca
     6421 taggcccaca gcgtcgcaat caccgccgcc atgaagttgt tgaacgcgtt gccggacgcc
     6481 gcgtgcggca acgcggtgcc gaaattcgac cacgatccgg cgccagagct gaagcagaac
     6541 ccggccacca ccaggatcag cagcaccttg agcacggtga agacaacctg aaagtccccg
     6601 gctttcttga tccccagata gttgaggccg gtgatcagcc aggtgacgcc gatcgcgaac
     6661 agctgcgacc acatcagcgg gaactgcccg cctatcgccg cggtgtcgag ccagtgaaag
     6721 gccggaaaaa tgccgagcgt acgcgccaga cccatggtca cggtgccgat cgaggccggc
     6781 ttcgcgaccg caaaccaggt ccacatgtac aggaagctca tgcgatcgcc gtaggcgccg
     6841 cgcaggtaaa cgtattcgcc acccgcatac ggcatcatgg cgccgagctc ggagtaggtc
     6901 atcacgccga acagtgacag cagaccgccg acgatccacg cggcgtacac caggagcgag
     6961 gatcccacgt cctgcatcat ctcggaaggc accagaaaaa tcccgctgcc aatgatggtc
     7021 ccgaccacga tcgcggtcgc gtgcgtaccc cccagcacgc gcggcagatc gggcgcggtc
     7081 cctgcaccgg tctcggacat ctgctgcgtc acgcctccaa cgataccgta tgcggcagca
     7141 gcctggcacg atccgcgctg ggccccacca ggatttcaat ctcccccggt tcgaacactc
     7201 gcgtcaggtc cgcgcccaga aatccaagct ccgccgctgg cagctgcagc gagaccgtgc
     7261 cgctttgtcc gggctgcaga agaatccggc caaacccttt cagttccagc actggccggg
     7321 ccacgctcgc cagccggtct cgcgcaaaga gaaataccgt ctcctcgccc tcacgtggtc
     7381 cgacgttcac caggtccacc aggatctgca gcgtgtcgct ctcgcgcgct cgctcgggcg
     7441 tgacccgcag gttgctgtag cggaactgcc cgtagctcag tccatggccg aaatgaatta
     7501 ccggttcatt cggtgagtcc agatatttgc tggtgtagtg atcagtcgga ttcgcgggcc
     7561 gaccgctcgg ccgctgcgcg aaaaacaccg gcacctgtcc catcgagcgc ggccaggaca
     7621 ccggcgtgcg cgccgacggc gagactttgc ccgtgagcac gtcggcgacc gcgtgaccgg
     7681 cttccgcacc cggaaaccaa gccgcgagaa tcgcgtccgc gcgctcgaac agccacggca
     7741 cgatcagcgg acgacccgag aacagcaccg caatgacggg aat
//
LOCUS       DATMRM010000249        15457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2307071, whole genome shotgun sequence.
ACCESSION   DATMRM010000249 DATMRM010000000
VERSION     DATMRM010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15457
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2307071"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1049)
                     /locus_tag="VNZ06_07215"
     CDS             complement(96..1049)
                     /locus_tag="VNZ06_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWW20578.1"
                     /translation="MPITFDGQVVIVTGAGQGLGREYALELGRRAAAVVVNDVAGLNT
                     KEGSAADAVVSEIQSAGGKAVASYDTVATADGGQAIVDRALEIFGTVDAVIHNAGVWR
                     HKLYHEMTADQLDPVLDVHLRGAFFVTQPAWSIMKEKGYGRIVLTSSSSGAFGREAGT
                     NYAAAKAGLLGLSRALALEGAHYGIRSNCILPIAPFRKRGPVPAALTERLTNAGLPRD
                     SGPELVAPMVAYLASAACAVNGEAFSAGGGRYGRVFIGVADGWLSPAGACATAEDIEA
                     HLDEIEDIGTYRVPASSWDELRDIGLLHSRRSKTPDGFNDA"
     gene            complement(1172..1636)
                     /locus_tag="VNZ06_07220"
     CDS             complement(1172..1636)
                     /locus_tag="VNZ06_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWW20579.1"
                     /translation="MTVQLPDPATADCLAVRSILALVADKWTVLIVGLLGDGPKRFNE
                     IKRMVDGISQRMLTFTLRGLERDGLVTRTVFPTSPQRVEYELTKRGGGLWEAVEPLGL
                     WARTHVNEILKARQQFDQKNLSTGRLASSASFPSRSARVAVNPYRPAQRSAK"
     gene            1775..2359
                     /locus_tag="VNZ06_07225"
     CDS             1775..2359
                     /locus_tag="VNZ06_07225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWW20580.1"
                     /translation="MSVISVIVGSTREGRFSEKPAKWIFQHLRKHEAVDARLLDLRDF
                     PLPFFDQPAPPAMPGRAPYQEGTVQRWTAAIAQSDGFIFVTPEYNYGTSAVLKNAIDW
                     VYPEWNRKAAGFVSYGSAMGARAVQQLRETLIELQVAPIRFSVHIPVATLWTHFKGGD
                     VDAGLAELAAPAGAMIDDLLWWTAALKRARVGAA"
     gene            2830..4818
                     /locus_tag="VNZ06_07230"
     CDS             2830..4818
                     /locus_tag="VNZ06_07230"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel family protein"
                     /protein_id="HWW20581.1"
                     /translation="MSSDLQATTGTAAAILRELPSVEVDADGVVSLRGYSNVTILVDG
                     KPSAQLSGAAAADALLQLSANDIEKIEIITNPPAQFKANGSAGIINIITKKRHEMGPS
                     GTAQASAGNNGRYVLNFNSGYAGKQLGLAGGLGLRQDERQRLITDNRAAIAPGTNSVA
                     ISQEDLAEHVRRSTPSIKAQIDYSFNDHQSGGVAFSERERSGGRHFDQFDESGLPDEP
                     PTSISNRYSNGHEWRLDDGEDVHFEQKLRQPDETLSFDLQRSSVRERERYAYTNIYTL
                     PVVSPSYDDLRLNQALVTTEFSADYVLPLSNGRSFKLGYDFEQDNSAFDNSGDNINAL
                     TGQAIVNPEITNDFHYLQQIHSVYASYLESMRMWTVLAGLRLEETITGFPQTAQSADS
                     THSYARAYPNIRVERPLTGASTLWASISRRVTRPDPEALNPFIDSQDTQNLRSGNPNL
                     RPQDTQSFELGDKFESKNLNFGLTGYLRRNRNSVTEVTQVVNTQVVLITKANLPRDTS
                     EGLEFTADGHFAPSLSYALSANLFHDQIDATALGAPGVKATTGINAKVSFDYHPTAAD
                     TAQISFSRSDRRLTPQGYVSGINLVNVGYKRQFGAGWAGLLTVSDLFNGQVFRRFVGT
                     PTLTDSYQRQQTGRIAYVGVTYTFGAGKKSKAGFEYDP"
     gene            4948..5985
                     /locus_tag="VNZ06_07235"
     CDS             4948..5985
                     /locus_tag="VNZ06_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20582.1"
                     /translation="MPKPVWAVTGIVPFYEISANPSNALKSRFTTLGVSISCYDDFTF
                     ELLSCPYSYQLVGVDDLTDPLIQDQLSFYPPFTELQQFGGHEHDQTTHPFYYLIPPAA
                     IPITVTGGTYQYQSSTLVTGNTFSVTAELIFSAPEVAGTIWERVDIGTPPGWYCVWPC
                     YTITDSLFHSTYFVGYEGFNQLDPTATLNGPTDYIVARKPNPDPAHPNANATWGDAYT
                     IVQLELMANDYRQITGNQLSINDMSLPYGGVFDIDAVTPGSAQTWHPPHNGHRIGYAF
                     DVNTTDAGGQPVVCNDGGDFDTAVEEFDGLISLDPATVPVQIHCEPQPDGSPDHIHIN
                     AINESNHLIIG"
     gene            6000..8087
                     /locus_tag="VNZ06_07240"
     CDS             6000..8087
                     /locus_tag="VNZ06_07240"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HWW20583.1"
                     /translation="MNRNALLFAAALVLGISVQRSVAQQTQLGIYPVPTFTAVTVQTA
                     VSFDPSTAHYFYNYTVTNPASNTGTIYSIEEDLTAPLNAFLPTGSGFTLPRGAAGPVL
                     FDNLWAQRKGVTSVPFNQQAVPFGSTAPSGWLGTLSVIATGGWHSRSVAAEIPPGQTL
                     SGFNLVSYGSPTIRAMTFKPEWILDLGPTGGEPTDAQAIQAAQIKQSLQVTVFVLGPS
                     VYNVDIGTLLSQLAKDVATAVQLGWISDATLAAQIQSQIHSAQTLYGSEGPDYNTYTA
                     LKTALNTVTSAAPGKLTTDGNNLVQLALSQVLRQVGNPSPGGPPQPPPPTPKVTITSP
                     VSRALSLPVGSTATITASVVDVAQGGTPLANYNVPLQITSGPNAGTQATLTSDANGTV
                     TYSYASANTGVDVVSLLANVTPAAGVRPLVVVKNPAADAASVVWDGGPDLVINEFTPP
                     VVNWASQASLHLTDTTTNIGNVAEQPSTTQYFMSTQSPVDPTAAVLLGGRPVPTLQPG
                     ASNFYQIDLPVPSQFQSPGTYYLLACANGNRAVAETNYQNNCQTRQATQMIHQTVVPP
                     VCTSAAPSEGSIWPPNHKYVSENVVGVTDPNRLAVTITISGIQQDEPVLGIGSGNTAP
                     DATGVGTSTAQVRAERSGTGNGRIYFISFGAVNTAGGQCAGTVQAGVPHDQGQGSTPV
                     DTGYRYDSTVVPL"
     gene            complement(8179..8775)
                     /locus_tag="VNZ06_07245"
     CDS             complement(8179..8775)
                     /locus_tag="VNZ06_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20584.1"
                     /translation="MRITEDSPDRLVLQEQTIWISVICGAAAVWIGAVSVLHQAWRPL
                     VGAALFACFGILFIRKSRVEISRSTHTVIIKITKPFNWRTVRLRFDEVKDVVIEPDAM
                     QSNRTPSCRLALTTSSGSQPLSEVYSGGYVRYESMRESVLAALGRPIADALEASLRHL
                     VTSRRIIEAVELLRSQQKMGLAAARQRVEEVQREIGSS"
     gene            complement(8782..10242)
                     /locus_tag="VNZ06_07250"
     CDS             complement(8782..10242)
                     /locus_tag="VNZ06_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWW20585.1"
                     /translation="MSATVPVPGDRPHRYQQIARFVTDLVQRGALAPGARAPSLREVS
                     RVHKVSLSTATQAYRLLEDRGVLLSRPQSGFFVARGASIVVAEPKPSQPTQRARNVSI
                     PRIPLALLEHAADPQFAPLGCAIPAATLLASGRLNRHLARLARTQGVARNVYTPPNGL
                     PELRQQIARRALQWGQALSPDHIVVTCGCTEALNLALDATVAPGETVATDSPAYFGLI
                     QILKARRLRILELPTDPVTGIDLDALRTALRRKLLRACVLPSSFSNPLGYTLTDPKKR
                     AILKLLAAHHIPLIEDDIYGDLYFGTERPAPFMALNEAPGAEVIYCSSFSKTLAPGYR
                     IGWVIAGDERIEGLLTRKFTQSLCSAPLPQLAMSEFLASEGYDNHLRRLRAVFSHTIV
                     QMRRIISERFPPGTRVTNPAGGFVLWVELPARLDSSRLLALALEQRICFVPGEICSAT
                     GRFRNHLRLSCGSPWQEISQAVMTLGAIASDAAARL"
     gene            10375..10740
                     /locus_tag="VNZ06_07255"
     CDS             10375..10740
                     /locus_tag="VNZ06_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017040012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWW20586.1"
                     /translation="MSHTAIRLEQKLSLFKETWAPKVIAEMNDYQFKLVKIVGDFIWH
                     DHPETDETFIVLQGELRLDLPDSSVRLGPGEMFVVPKGVRHKPFAEREVHLMLIEPRG
                     VLNTGHEGGERTAPSDAWI"
     gene            10737..11669
                     /locus_tag="VNZ06_07260"
     CDS             10737..11669
                     /locus_tag="VNZ06_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HWW20587.1"
                     /translation="MSSTQSGRQLRYVTVDVFTQRVFGGNPLAVVFDAAALRTTQMQA
                     IAAEFNYSETTFVLPATAPGCAVQVRIFTPRAELPFAGHPNVGTAVCFARECERQNLV
                     IPEPLVFEELAGPIRITVLRDAGRISGAEISAPSPLSIGASIGAKDAAQCLELAASDI
                     AAQIHAPCVVSVGLPFLVVELASRSALARAAPNLEAHQRLLPAVGTDGVFAYTRGAGE
                     GELHARMFAPVGGIREDPATGSASAALIGLLASLRTAHTEERSWRIEQGVEMGRPSLI
                     CGRTEKRSGNVAGVHISGGAVPVMQGQLQLPPET"
     gene            complement(11685..12320)
                     /locus_tag="VNZ06_07265"
     CDS             complement(11685..12320)
                     /locus_tag="VNZ06_07265"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWW20588.1"
                     /translation="MANSRQQAIEAAERLFRAQGYAGTGLAQIIAESGSPKGSFYFNF
                     PGGKHELALEALKLYGERLDSRIRESAAAHGEDPTEFVRYVCARQARELEASGWTLSC
                     LAQQLANEFAPGDDEVTRAVARVMQTWIASMASVMRRAAASDQEATLLGTAFISGLSG
                     ARGLARVVRSKAPFEAVADSMVRMLRSVATRSAAKTARRRTKSARTAVTTH"
     gene            12500..12856
                     /locus_tag="VNZ06_07270"
     CDS             12500..12856
                     /locus_tag="VNZ06_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HWW20589.1"
                     /translation="MNSIHKALLASLLLNAATLLVSQAAMAKDITAAVAPPADRHEAV
                     PKPRAGYVWAPGHWEWTGRFYSWTSGTWVVQRRGQQWVADRWEQVGSQYHYLPGHWSA
                     DNAADAGSTLQAQDSR"
     gene            complement(12872..13090)
                     /locus_tag="VNZ06_07275"
     CDS             complement(12872..13090)
                     /locus_tag="VNZ06_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20590.1"
                     /translation="MAVPHAGTRAARARRARLAVVRRAALICYFVGAATDLLIGAFNP
                     YGWCSVLLSAALPGAPKKSPLTRAGFMH"
     gene            13176..14198
                     /locus_tag="VNZ06_07280"
     CDS             13176..14198
                     /locus_tag="VNZ06_07280"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWW20591.1"
                     /translation="MNPITKRAGLIAVVLVLVGAAAVLIWRGHRHVAQGELLLYGNVD
                     LRQIDLPFNGSERIADVDAQEGDHVRAGQVLARLDASRLQPQVAKAQAELAAQQQVVE
                     RLQHGNRPEEIAQARASVEAAQAESDDARAHYQRLSSLSDRSDGRAVSRQDLDSARAS
                     LDNTQAKLQMNQKALALEVAGARKEDIAQAQAQLRSDQAQLALLQQQLRDAELRAPVD
                     AVIRARLMEPGEIASPQKAVFTLAITDPKWVRAYVDESNLARIREGLAASVTVDGLPG
                     LSIPGWVGFISPIAEFTPKSVETAELRSSLVYEVRVFVKDPQDRLRLGMPATVHIARA
                     DKASAQ"
     gene            14195..>15457
                     /locus_tag="VNZ06_07285"
     CDS             14195..>15457
                     /locus_tag="VNZ06_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWW20592.1"
                     /translation="MNSSQPALQARGLGKSFLQATGERVAALKDLSIDVEHGALTALV
                     GPDGAGKTTLMRLATGLMLADQGTLTVLGIDVRERPQAVQELISYMPQRFGLYEDLSV
                     AENMDLYADLHGVTPEERAQRYPMLMEMTALTNFRRRLAGRLSGGMKQKLGLACTLVR
                     SPQLLLLDEPTVGVDPLSRRELWEIVQRLVREQSLTVLVSTSYLDEAERCQRAIVLHR
                     GQLLVQAAPLEVAQRAAGRVYRLTPAANQPARVLQSTLLEQADTVDAVPEGGRVRYVR
                     SQLSETALPPGLTAEPIAPSFEDGFMTLLAGKVERERGAHIAVKAVARRDGASRAVRV
                     DRLVKRFGQFTAVDQISFDVQAGEIFGLLGPNGAGKTTTFRMLCGLLTPSAGALYVAD
                     TDVRKAPTSARARLGYVAQKFSLYGALSV"
BASE COUNT         3043 a         4704 c         4790 g         2920 t
ORIGIN      
        1 caatcgctac aatgtggtga ccatgcgtgg tgctttcatc cggcgcaata tcgaggagtt
       61 cgagcgtgca gactccggcc gtccgaagag cggggtcagg cgtcgttgaa gccatcaggg
      121 gtcttgcttc gccttgaatg cagcagtccg atatcgcgca gctcgtccca cgacgaagcc
      181 ggcacccgat aggtgccgat gtcctcgatc tcgtcgagat gcgcctcgat gtcctccgcg
      241 gtggcacacg cgcctgcagg actgagccag ccatcggcta cgccaatgaa cacgcgtcca
      301 tatcgcccac cgccagctga aaacgcctcc ccgttcactg cgcaagcggc gctcgcgaga
      361 taggccacca tcggcgcaac aagctcgggc cctgagtcgc gtggcaaccc cgcattggtg
      421 agccgttcgg tcagcgcggc cggcaccggc ccacgtttgc gaaatggggc aatcggaagg
      481 atgcaattgg atcggatgcc ataatgagca ccctccaacg ccagggcgcg gctcaggccg
      541 agaagcccag ctttggccgc ggcatagttg gttcccgcct cgcgaccaaa cgcgccactg
      601 ctcgagcttg tcagaacgat tcttccgtag cccttttcct tcatgatcga ccatgcaggc
      661 tgcgtcacaa aaaacgcgcc acgcaaatgt acatcgagga ccggatcgag ctggtctgcc
      721 gtcatctcgt ggtaaagctt gtgtcgccaa acgccagcat tgtggatgac agcgtcaacg
      781 gtgccgaata tctcgagcgc tctgtccacg atcgcttgac cgccgtctgc cgtcgctacg
      841 gtgtcatagg acgccaccgc tttgcctcca gcggattgga tctcagacac aaccgcgtcg
      901 gcagccgaac cctccttagt attcaagccc gcgacgtcat tgacgacaac cgcagctgcg
      961 cgtcgcccca gctcgagtgc gtattcgcgc ccgagtccct ggcccgcgcc agtcacgatc
     1021 accacctgtc cgtcgaatgt aatcggcatc gcgattccct cttatctccg ccaacgcaca
     1081 gacggtcaga aatgttgtgc tcgtcttggt cccatggatc ttgggagcaa tttggtcaat
     1141 caagaacagg cggcgggtca gcactccagt gctatttcgc ggatcgttgc gcggggcgat
     1201 atgggttgac ggcgacgcgt gcggagcggg acggaaacga tgcagatgac gcaagcctgc
     1261 cggtgctgag gttcttctgg tcaaactgtt gccgcgcttt gaggatctcg ttcacgtgag
     1321 tgcgcgccca caaacctaac ggttccacgg cttcccacaa gccgccgcca cgtttggtca
     1381 actcgtactc cacacgctgc ggactggtgg ggaacacggt gcgcgtcacc aggccgtcgc
     1441 gttctagtcc acgcagcgtg aaggtcagca tccgctgtga aataccatcc accatgcgct
     1501 tgatctcatt gaagcgtttc gggccatcgc cgagtaaccc gacgatgagt acggtccact
     1561 tatcggcgac gagcgccaga atggatctga cggcgaggca gtccgcggtg gccggatcgg
     1621 ggagttggac ggtcacttca gtgtgctcag gtatcagacg tgtgcgttct tgcggaacat
     1681 aacagcagta tataacttag tccaggtcga aagtttatac cggtctggca tcgaccgacc
     1741 ttgtttcttt caacgagccg tgaggagtca aagcatgagc gtcatttccg tcattgttgg
     1801 aagcacgcgc gaggggcgtt tctcggaaaa gcccgcaaag tggatttttc aacacctcag
     1861 gaagcacgag gccgtcgacg cccggctgct tgatctgcgc gactttccgc tgccgttctt
     1921 cgatcagccc gcaccaccgg cgatgcccgg gcgtgcgcct taccaggagg ggacggtgca
     1981 gcgctggacc gcggccatcg cgcaatctga tggctttatc ttcgtcactc ccgagtataa
     2041 ctatggcacc tccgcagtcc tgaagaatgc gatcgactgg gtctatcccg agtggaatcg
     2101 caaggcggcc ggcttcgtga gctatggttc agccatgggg gcgcgtgccg tgcaacagct
     2161 gcgtgagacg ctcatcgagc ttcaagtcgc cccgattcgc ttctcggtcc atatcccggt
     2221 cgccaccctg tggactcact tcaagggcgg cgatgtcgac gcgggactgg ccgagttggc
     2281 cgcgcccgct ggggcaatga tcgacgacct gctgtggtgg accgcagcct tgaagcgcgc
     2341 gcgcgtcggc gcggcctgac cgccggcaaa gcggcgcatt tgcgaaggga aactgcgggg
     2401 acaagcggaa gtggaatcgg cgtcgcgagc gcgggcagac tcggcggtgc agcgccagcc
     2461 ttaccgccta tacaaaagtg tcacgcaaag cctgcttgtg aggtagtgag gtaaatgtcg
     2521 ctgtgctaaa cctggtgcgc ccaataggag ggacttggtc tgcatgtgtc ttagcagggg
     2581 cgcgccgatt gtgcgcagga ataagaatgc tcttcgatcc gcgcgcatcg aagcctacag
     2641 cagatgagtt gggcgtttcg agggctaacc agcgcggccg gcggtattgc tgcgatacat
     2701 ttgtgcgggc cagctttcgc gaccgagccc gagccgagcc ccaaacccag cgccgacgtt
     2761 ttagagccgg tcgttgtcgc cgctcaaaga gcacccgttc aacgaacgat cgatcgcacc
     2821 gtctatgacg tgtcgagcga tcttcaggcc acgaccggca ccgccgccgc catcctgcgg
     2881 gaactgcctt cggtcgaagt ggacgccgat ggcgtcgtga gtctgcgcgg ctacagcaac
     2941 gtcaccatcc ttgtcgatgg caaaccgtcc gcgcagcttt ccggcgcggc cgctgccgat
     3001 gctctgctgc aactttcagc aaacgacatc gagaagatcg aaatcatcac gaacccgccg
     3061 gcgcagttca aagccaacgg ctcggccggg atcatcaata tcattaccaa gaagagacac
     3121 gagatgggcc cctccggcac ggcgcaggcc agtgccggta ataatggccg ctacgtcctg
     3181 aacttcaata gcggctacgc cggaaaacaa ctgggcctgg caggcggact cgggctgcgt
     3241 caggatgaac ggcagcgtct catcaccgat aaccgtgccg ccatcgcccc gggcacgaac
     3301 tctgttgcga tcagtcaaga ggatctggct gaacacgtgc gccgatcaac gccgtcgatc
     3361 aaggcccaga tcgactatag cttcaatgat catcaatcgg gcggcgtggc attcagtgaa
     3421 cgcgaacgca gcggcggtcg ccatttcgat cagtttgatg aaagcggctt acccgacgag
     3481 ccgccaacga gtatttccaa ccgctacagc aatggccacg aatggcgtct ggacgacggt
     3541 gaggatgtgc atttcgagca gaaactcaga caacccgatg agacgctcag cttcgatctg
     3601 cagcgctcca gcgtgcgtga gcgcgagcgc tacgcctaca ccaatatcta tacgctgccg
     3661 gtggtcagcc ccagttacga cgatctgaga ctaaaccagg cccttgtcac caccgagttc
     3721 agtgcggact acgtcttgcc gctatcgaac ggccgaagtt tcaagctcgg ctatgatttt
     3781 gagcaggaca acagcgcctt tgacaactcc ggcgacaaca tcaatgccct gaccggtcag
     3841 gcgatcgtca atcccgagat caccaacgac tttcactact tgcagcagat ccactcggtc
     3901 tacgccagct atcttgagtc gatgcgcatg tggacggtct tggctgggtt acggttggaa
     3961 gagaccatca ccggcttccc gcagaccgca caaagtgccg atagcacgca cagctacgct
     4021 cgtgcttatc cgaatattcg ggtggagcgg ccattgaccg gggcatccac gctctgggcg
     4081 agcatcagcc gccgggtgac gcgacccgat cccgaggcgc tgaatccgtt catcgatagt
     4141 caggataccc agaacctgcg ttcggggaat ccaaatctgc gcccgcagga cacacagtct
     4201 ttcgaactcg gcgacaaatt cgagtccaag aatctgaact tcggtctgac cggctacttg
     4261 cgacgcaatc gcaacagcgt cacggaagtg acacaggtgg tgaacacgca ggtggtccta
     4321 atcaccaagg ccaacctgcc tagagacacg tccgaagggc ttgaattcac agccgacggg
     4381 cacttcgcgc cgtcgctgtc ctatgctctg agcgcgaacc tgttccacga tcagatcgat
     4441 gccacggcgt tgggcgcgcc cggcgtgaaa gctaccaccg gcatcaacgc caaggtcagt
     4501 tttgactacc acccaacggc ggccgacacc gcgcagatct ccttcagtcg atcagaccgg
     4561 cgtctgacgc cccaaggcta tgtcagtggc atcaatctgg tgaacgtagg ctacaaacgc
     4621 caattcggag cgggctgggc agggctcctg accgtgtcag atctctttaa cggccaagta
     4681 tttcgtcgct tcgtgggcac gccaaccttg acggatagct atcagcgcca acagaccggg
     4741 aggattgcct acgtgggtgt cacctacacg ttcggtgcgg gcaagaagag caaggcggga
     4801 tttgagtacg acccatagcg tcgcaccggg cgtgccaatg tgtgcgccgg tctcaccaat
     4861 gagaccgctt accccgacat cttccggtaa gtgtggcgag caggcctgtg acgcagcgcc
     4921 ctgtccgtcg ccagatgatc cgtgacgatg cctaaaccgg tgtgggcggt caccggcatc
     4981 gttccgttct acgaaatttc tgcgaacccg tcgaatgctt tgaagagccg gttcactact
     5041 ctgggagtca gcattagctg ctacgacgat tttacgtttg aattgctgtc ctgcccttat
     5101 tcctaccagc tggtgggcgt cgatgatctg acggacccat tgatacaaga tcaactctca
     5161 ttttatccgc cattcacaga gctacagcag ttcggcggac acgagcatga tcaaaccacc
     5221 catcccttct actatctgat accgccggcg gcaatcccga ttaccgttac tggcggtacg
     5281 taccaatatc aatcgtcgac gctggttacc ggcaacacat tctcagtgac agcagagctc
     5341 atattctcag ctccagaggt tgcggggact atctgggagc gagtcgatat tggtacacct
     5401 ccgggctggt attgcgtatg gccctgctac acgatcacgg attcgttgtt ccacagtacc
     5461 tatttcgtgg gttacgaagg gttcaaccag ctcgacccga ccgcgaccct aaatggtcct
     5521 acggactata ttgtcgctcg caagcccaac cccgaccctg ctcacccgaa tgcgaatgcc
     5581 acctggggtg atgcatacac catcgtgcag ctcgagctga tggccaatga ctatcggcaa
     5641 atcacgggaa atcagctctc catcaacgac atgagtttgc catacggagg ggtttttgac
     5701 atcgacgctg tcacccccgg ttccgcgcaa acctggcacc caccgcacaa cggtcatcgc
     5761 attggatacg cattcgatgt caacacgacg gatgcgggcg ggcaacccgt ggtgtgcaat
     5821 gatggaggtg attttgatac cgctgtcgag gaattcgatg gacttatcag tttagatccc
     5881 gccaccgtgc cagtccaaat tcactgcgaa ccacagccgg acggatcgcc ggaccatatt
     5941 catattaatg ctatcaatga gtccaatcac ttaatcatcg ggtagttgga gtgttgtcta
     6001 tgaatcgcaa tgcactgcta ttcgctgcgg ccttggtgct gggtatttca gtacagagat
     6061 ccgtcgccca gcaaacccaa ctgggtattt atccggtgcc gacatttacc gccgtcactg
     6121 tgcagacggc cgtttcgttc gatcctagca ccgctcacta tttttacaac tacaccgtga
     6181 ccaacccggc cagcaacacg ggcaccattt acagcattga agaggatctg accgcgccac
     6241 tgaacgcatt cctcccgacc ggtagtggat tcacccttcc gagaggcgca gccggcccag
     6301 tactctttga caatctgtgg gcacagcgca aaggagtgac gagcgtgccg ttcaatcagc
     6361 aggcagtgcc cttcggatcc acggcgccga gtggctggct tgggactctg tctgtcatcg
     6421 cgacgggcgg ttggcattcg cggtcggtcg cagcggagat tccaccaggt cagacattga
     6481 gcggcttcaa cctcgtcagt tacggttcgc cgacgattcg tgcgatgacg ttcaagccgg
     6541 aatggatttt ggatcttggg cctacgggtg gcgagccgac cgacgcccag gcgatccaag
     6601 ccgctcagat caagcagtct ctccaggtca ctgtctttgt gctgggtcct tcagtttaca
     6661 acgtagacat tggaacactc ttgagccaac tggccaagga tgtcgccacg gcagtgcagc
     6721 tgggatggat ttccgacgcg acgcttgctg cgcagatcca gtcgcagatt cattccgccc
     6781 agacgcttta tggcagtgaa gggccggact acaacacgta tacggcattg aagaccgcat
     6841 tgaacacggt cacttctgcc gcaccgggga agctgacaac cgatggaaat aatttggttc
     6901 aattggccct gtctcaggtg ctgcgccaag tgggcaatcc gtcacccggc ggaccgccgc
     6961 aacccccgcc ccctacccct aaggtgacga tcacgagccc ggtgtctcgt gcactgagct
     7021 tgccggtggg ctcgactgcg accattactg cgagcgtggt agacgtcgcg cagggcggaa
     7081 cacctcttgc caactataac gtgccgctgc agatcacctc cggccccaat gctggtacgc
     7141 aggcaacatt gaccagtgat gcgaacggta ctgtgacgta ttcctacgca agcgcaaata
     7201 ctggggtcga cgtggtcagc ctgctggcga atgtgacgcc cgccgcgggt gtgcgcccac
     7261 tggtcgtggt caagaacccg gccgctgatg ctgcatcagt cgtctgggat ggcggtccag
     7321 acctcgtgat caacgaattc acgccaccgg tcgtcaactg ggccagccaa gctagcctgc
     7381 atctgaccga cacgacgacc aacatcggca atgtcgcgga acaaccctcg acgactcagt
     7441 atttcatgtc cacgcagtcg cccgtcgatc ccaccgcagc cgtcctgttg ggcggaaggc
     7501 cagtgccgac tctgcagcct ggggcgtcca atttctatca gatcgatttg ccggtgccga
     7561 gccagtttca gtcacccggc acctattatc ttctcgcctg cgcgaacggc aaccgtgccg
     7621 tggctgagac caactatcag aataattgcc agacccggca agccacacag atgattcatc
     7681 agaccgtggt accacccgta tgcacgagtg cagcaccctc cgagggcagc atttggccgc
     7741 ccaatcacaa gtatgtgtcc gagaatgtcg ttggggtcac tgaccctaac cgtctggccg
     7801 taactatcac gatctccggc attcaacagg acgagccggt tctaggaatc ggcagcggca
     7861 acacggcccc tgacgcgaca ggcgtcggca cgtcgaccgc gcaggtgcgt gcagagcgct
     7921 ctggcacggg caatgggcgt atttacttca tttcgttcgg ggccgtgaat actgctggcg
     7981 ggcaatgtgc agggacagtt caagccggtg tcccgcatga tcaagggcaa ggttctactc
     8041 ccgttgatac cggataccga tatgactcga cggttgtacc actgtagccc tggctaggga
     8101 cgcctactga ctctacgttc aaaggattga tgtgactgtg gggattgtgc tcaagattcc
     8161 ctgccgtatt caatcaatct agcttgaacc gatctcgcgc tgcacttcct ccacgcgttg
     8221 acgtgcggcg gccaggccca tcttctgctg tgatctgagc agttccacgg cctcgatgat
     8281 tcggcgcgag gtcaccagat ggcgcagtga cgcctcgagt gcgtcggcga tgggtctgcc
     8341 gagtgccgcg aggacacttt ctcgcatcga ctcatagcgc acgtagccac cggaatacac
     8401 ctcgctcaac ggttgcgacc cggaggacgt ggtgagagcg agccgacagg agggcgtgcg
     8461 attgctctgc atcgcatccg gctcgatcac gacatctttg acctcatcga atcgcaggcg
     8521 tacggtgcgc cagttgaaag gtttggtgat tttgatgatc accgtgtgtg tggaccggct
     8581 gatctcgacg cgtgacttcc ggatgaacag gataccgaag cacgcgaaca gtgcggcacc
     8641 caccagcggt cgccaggcct gatgcagcac ggagaccgct ccaatccaca ccgctgcggc
     8701 accgcagatg acactgatcc agatcgtctg ctcctgcagt acgagccggt ccggcgaatc
     8761 ttcggtgatt ctcatggcct atcataggcg cgcggcggca tcgcttgcga tggcaccgag
     8821 tgtcatgacc gcctgggaga tctcctgcca cggactgcca cagctcaggc gcagatgatt
     8881 gcgaaagcgg ccggtggccg aacagatctc acccggtaca aaacagatgc gttgctccag
     8941 ggccagcgcc agcagccggc tgctgtcgag ccgggcgggc aattcgaccc acagcacaaa
     9001 acctccggca ggattggtga cacgcgtgcc gggcgggaag cgttcgctga tgatgcgtcg
     9061 catctgaacg atcgtgtgcg agaacaccgc acgcaagcgg cgcaggtgat tgtcatagcc
     9121 ctcggatgcc agaaactcgg acatcgccag ttgcggcagc ggagcgctgc agagcgactg
     9181 ggtgaatttc cgggtgagca gcccctcgat acgttcgtcg ccggcgatga cccagccgat
     9241 ccgatagccg ggcgcgaggg tcttggaaaa ggagctgcaa tagatcacct cggcgccggg
     9301 cgcctcattg agcgccatga aaggtgcagg tcgctcggtg ccgaagtaca gatccccata
     9361 gatatcgtct tcgatcagcg ggatatgatg cgcagccagc agcttcagga tcgcgcgctt
     9421 cttcgggtca gtcagggtgt aaccaagcgg attgctgaaa ctggatggca gtacgcaggc
     9481 gcgcagcagt ttgcgacgaa gcgcggtgcg caacgcatcc agatcgatgc cggtcaccgg
     9541 atccgtcggc aattccagaa tccgcaggcg acgtgccttg agaatctgta tgaggccgaa
     9601 gtaggcgggc gagtcggttg ccaccgtctc accgggcgcc accgtggcat ccagtgccag
     9661 attcagggcc tcggtacagc cgcaggtgac gacgatgtgg tcgggcgaca gtgcctgtcc
     9721 ccattgaagc gcccgacgcg caatctgctg gcgcagctcg ggtagcccgt tcggcggcgt
     9781 atagacattg cgtgcaaccc cttgcgttcg ggccaacctc gccagatgac gattcagacg
     9841 acccgatgcc agcaacgtgg cggcagggat cgcgcatcca agcggtgcga actgcgggtc
     9901 cgcggcgtgt tcgagcagtg cgagcgggat ccgcggtatc gagacgttgc gcgcgcgctg
     9961 cgtcggttgg gagggtttcg gctcggccac gacaatcgag gcgccgcgcg cgacgaaaaa
    10021 cccggactgc ggtcgagaca gcagaacccc gcgatcctcc agcagccggt aggcctgcgt
    10081 ggcggtcgat agactcacct tatgaacccg ggatacctcg cgcagtgacg gcgcccgtgc
    10141 gccaggggcc agcgccccgc gttgcaccag gtcggtgacg aagcgggcga tctgctgata
    10201 tcgatgagga cggtcgccgg gcactggcac ggttgcggac acggcttgct ccgatgaacg
    10261 gctagcaaaa ctgtactggt cgagattgca gtctactgta gctgttctgg tctggccaca
    10321 atgactagca ttcatgagtg gcgctgacca gcgctgtgcg cggggagtga ttcgatgagc
    10381 cacacggcta tccggctcga acaaaagcta tcgttgttca aggagacctg ggcgccgaag
    10441 gtaatcgcgg aaatgaacga ctaccagttc aaactggtga aaatagtcgg cgatttcatt
    10501 tggcatgatc atccggaaac ggatgaaacc ttcatcgtgc tgcagggcga gttgcgcctc
    10561 gaccttcctg acagcagcgt gcggctcggt ccgggcgaga tgttcgtggt gccgaagggc
    10621 gttcggcaca agccgttcgc cgagcgagag gtgcatctga tgctgatcga gccacgcggc
    10681 gtgctcaata ccgggcacga aggcggcgag cgaacggcgc cgagtgacgc ctggatatga
    10741 gcagcacgca gtcaggccgg cagctgcgct atgtcacggt cgacgtcttc acgcagcgcg
    10801 tatttggcgg caaccccctg gccgtggtgt ttgatgcggc cgctctgcga accacacaaa
    10861 tgcaagcgat cgcggccgag ttcaactatt ccgagaccac tttcgtgctg cccgcgactg
    10921 caccgggatg cgcggtgcag gtcagaatct tcaccccgcg cgcggaactg ccgttcgccg
    10981 gacaccctaa cgtgggtacc gcggtgtgtt tcgcccgcga gtgcgagcgg cagaacctgg
    11041 tgattcccga accgctggtc ttcgaggagc tggccgggcc gatacgcatc acggtgctgc
    11101 gtgacgcggg acgaatcagc ggagctgaga tcagtgcgcc atcgccgctg agcatcggtg
    11161 cgagcattgg cgccaaggac gcggcgcaat gcctggagct ggccgcgagt gacattgcgg
    11221 cgcaaatcca cgccccctgc gtcgtgtcgg tcggactgcc gtttctggtg gttgaacttg
    11281 catcgcgcag cgcactggcc cgtgcggcgc cgaatctgga ggcgcatcag cggctgttac
    11341 ctgcagtggg caccgatgga gtcttcgcgt acacgcgcgg ggcaggcgag ggcgagctgc
    11401 atgctcgcat gttcgcgcct gtgggcggca tccgcgagga tcctgccacc ggcagtgcgt
    11461 cggcggccct gattgggctg ctggccagcc tgcgcacggc ccacacggag gaacgatcgt
    11521 ggcggatcga acagggtgtc gagatggggc gaccgagcct gatctgcgga cgcaccgaaa
    11581 aacgatcggg caacgtcgct ggcgtgcaca tctctggcgg tgccgtgccg gtcatgcagg
    11641 gccaattgca gctgccgcct gagacctgac cggggggcgg tgaactagtg ggtcgtgaca
    11701 gcggtgcgcg cgctcttcgt gcgacggcgc gcagtcttgg cagccgatcg cgtggcgacg
    11761 ctcctcagca tccgcaccat gctgtcggcg accgcctcaa acggtgcttt gctgcgaacg
    11821 acacgtgcca ggccgcgcgc accgctcaag ccggaaatga aggccgtacc cagcagcgtc
    11881 gcctcctggt cggaggcggc ggcgcgccgc atcacgctcg ccatgctcgc tatccaggtc
    11941 tgcatcaccc gcgcaaccgc gcgggtgacc tcatcgtcgc ccggagcgaa ttcattcgcc
    12001 agttgctgcg ccaggcagct gagcgtccaa cctgaggctt ccagttcgcg cgcctgcctg
    12061 gcgcacacgt agcgcacaaa ttcggtcgga tcctcaccgt gggcagcggc tgattcccgg
    12121 atgcggctgt cgaggcgctc gccgtagagc ttcagcgcct cgagcgccag ctcatgtttg
    12181 ccgcccggaa agttgaagta gaacgagccc ttgggactgc cgctttccgc gatgatctgg
    12241 gcgagcccgg tcccggcata gccctgcgcg cgaaacagtc gctccgccgc ctcaatcgcc
    12301 tgttgtcgtg aattcgccat gacctactat ggtaatcgcc atagcggccg gagtccatag
    12361 agggtcacgc tatggtgatc gccataacct tatgtcgccg gatgcatagc tcgactatgg
    12421 caatcgccat agtatacgag cacgcgatcg aagcatgcgg tcgtgctcca cccaggcact
    12481 acagagaggt tttagggata tgaattccat tcacaaggct ttgctggcta gcctgttgct
    12541 caatgccgcg acgctactgg tatcacaggc ggcgatggcc aaagacatta cggcggccgt
    12601 ggcgccgcct gccgatcgac atgaagcggt gccaaagccg cgcgccggct atgtttgggc
    12661 acccggacat tgggagtgga ccggccgctt ctatagctgg acctcgggca cgtgggttgt
    12721 gcagcgccgc ggccagcagt gggtggctga tcgctgggag caggtcggct cgcagtacca
    12781 ctacctgcca ggtcattgga gcgcggataa cgccgcggac gcaggttcca cgttacaggc
    12841 acaggacagc cgatagtccc ctcatcgagc gtcagtgcat gaagcccgct ctggtgagcg
    12901 ggcttttttt tggtgcgccc ggcagggctg cactcagcag cacgctgcac cagccgtacg
    12961 gattgaaagc accgatcaac aggtcggtcg cggcgcccac gaagtagcag atgagggcgg
    13021 cacggcgcac gaccgcgagg cgtgcgcgcc gtgctcgtgc agcgcgagtg ccagcgtgtg
    13081 ggacagccat gtttcagttg tcaaattcca tggcctcggg cgaagatgac ctcggggtat
    13141 gacccatcgc cggacaaggc cgcacaggct ggcaggtgaa tccaattact aagcgcgccg
    13201 ggctgatcgc cgttgttctt gtgctcgtgg gcgcagcggc agtgctgata tggcgcgggc
    13261 atcgccacgt cgcgcagggc gaactccttc tatatggcaa cgtcgatctg aggcagatcg
    13321 atctgccgtt caacggcagc gagcgcatcg ctgacgtcga cgcgcaggaa ggcgatcacg
    13381 ttcgcgccgg gcaggtgctt gcgcggctgg atgcctcgcg cttacaaccg caggtggcga
    13441 aagcgcaagc cgaactcgcc gcgcagcagc aggtggtcga gcgtctgcag catggcaacc
    13501 ggcccgaaga gatcgcgcag gcgcgcgcca gcgtggaggc cgcccaggcc gaatctgacg
    13561 atgcgcgggc gcactatcag cgactgagtt cgctctccga tcgctccgat gggcgtgctg
    13621 tcagccggca ggatctggac tctgccaggg catcactgga caacacgcag gccaagctgc
    13681 agatgaatca gaaagccctg gcgctcgagg ttgccggagc gcgcaaggaa gacatcgcgc
    13741 aggcgcaggc gcagctgcgc tccgaccagg ctcaactggc gctgctgcag cagcaactgc
    13801 gcgacgccga actgcgtgca ccggtcgatg cggtgatacg cgcgcgcctg atggaacccg
    13861 gtgagatcgc ctcaccgcag aaggcggtct ttacgctcgc gatcactgat ccgaagtggg
    13921 tccgcgccta tgtggatgaa agtaatctgg cccgaatccg cgaagggttg gcggcgagcg
    13981 tgacggttga cggcctgccg gggctgtcga ttcccggatg ggtcgggttt atttcgccga
    14041 ttgccgagtt cacgccgaaa tcggttgaaa cagcggaatt gcgctcgagc ctggtctatg
    14101 aggtgcgtgt gttcgtcaag gacccgcagg accggctgcg tctggggatg cctgccaccg
    14161 tacacatcgc gcgtgccgac aaggcgagtg cgcaatgaat tcgtcccagc cggcgttgca
    14221 ggcgcggggg ctgggcaagt ccttcctgca ggcgaccggg gagcgggtag cggcactcaa
    14281 ggatctgtcg atcgacgtgg agcacggcgc tctcacggcg ctggtcggcc cggacggcgc
    14341 tggcaagacc accttgatgc gactggcaac cggcctgatg ctggcggatc agggcacatt
    14401 gaccgtactc ggtatcgatg tgcgcgagcg tccgcaggcc gtgcaggagc tgatcagcta
    14461 tatgccgcag cgctttggtc tgtatgagga cctgagcgtc gccgagaaca tggatctgta
    14521 cgccgatctg cacggcgtca cgcccgagga gcgcgcgcag cgctatccga tgctgatgga
    14581 gatgacggcg ctgacgaatt ttcggcgacg tctggccggg cgattgtccg ggggcatgaa
    14641 acagaagctc ggacttgcct gcaccctggt gcgatctccg cagctgctgc tgctggatga
    14701 accgacggtc ggagtcgatc cgctgtcgcg ccgcgaactg tgggagattg tgcagcgact
    14761 ggtccgcgaa cagtcgctga cggtgctggt gagcacttcc tacctggatg aggcggagcg
    14821 ctgtcagcgc gcgatcgttc tgcaccgggg ccagctgctg gtgcaggcag ctccgttgga
    14881 ggtcgcgcag cgcgccgccg gccgcgtcta ccggctgacc cctgcggcga atcagcccgc
    14941 gcgtgtactg cagtcaacgc tgctcgagca ggccgatacc gtggatgccg tgcccgaagg
    15001 cggccgtgtt cgctacgtgc gctcgcagct gagcgagacg gcgttgccgc cggggttgac
    15061 tgcggagccg atcgcgccga gttttgaaga tggcttcatg acgctgctgg cgggcaaagt
    15121 cgagcgcgag cggggagccc acattgcggt caaggcggta gcgaggcgcg atggcgccag
    15181 tcgcgccgtg cgcgtggatc gactggtgaa gcgatttggc cagttcacgg cggtggatca
    15241 gatttcattc gacgtgcagg ccggtgagat cttcggcctg ctgggtccca acggcgcggg
    15301 caagaccacc acctttcgca tgctgtgcgg actgctgaca cccagcgccg gcgcgttgta
    15361 cgtggccgac acggacgtgc gcaaagcgcc gacctcggca cgggcgcgtc tggggtacgt
    15421 ggcgcagaag ttctcgctct atggtgcgct gagtgtc
//
LOCUS       DATMRM010000250         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_468918, whole genome shotgun sequence.
ACCESSION   DATMRM010000250 DATMRM010000000
VERSION     DATMRM010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468918"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VNZ06_07290"
     CDS             complement(<1..1061)
                     /locus_tag="VNZ06_07290"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWW20593.1"
                     /translation="MRRVRTGQRLLLSLGLMILGPALAADLFGPNADPKFRPQLRKFE
                     SPQALDAATQELHHGIWELYSGAYFAGTDVRARTVRDDLDEWVLTPARAQKIAALESQ
                     ARVQEAGHDQARMRTTLSEAATLFEVETYRVFLLGLYWGWQNRIGAHATNFDKLAALL
                     SQDETAAARPPLDAEVRTFAGQLTAGIALTDGDLQKQATALQQLEDTAQRVLRAYNDQ
                     RAGLALVYGRHEREAHKPPLARVRATPCPEATLPAPGNSQVKVLGSKGDLEEFYPVAA
                     KHDYFEGSVVVEVAVSAAGCADSAAVYDTSGVQSLDEAAVNYVLTACRFRPAAVADQA
                     VAASLLMRIKFKLSNPPPL"
     gene            complement(1068..>2013)
                     /locus_tag="VNZ06_07295"
     CDS             complement(1068..>2013)
                     /locus_tag="VNZ06_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20594.1"
                     /translation="DGEIWYCKDGGPGVTLFRLTYPEDIPAAHRYSDSKEFLDLLLKG
                     INLPRQVGTQAVRITSLGAAQQDALVHDHYGRTWQLRIWSLGFADVSLLAIALPTPDG
                     YVGMASVVPGGLQDLQAEQIKFIADYFYLTYRGTLAQWQAFLARRDLMPDAFERVKLQ
                     YSPGGPLHLESARLQLDTTGLVNLDAKSSLDLQMNYLVDRGKSQWDIAGVVLRPERGK
                     KSHLALYRQSKPADDATKERRDRWEHMSQRNGEFAGTLQHDDSLTEFWIRTVAAGPGA
                     AGGAGPLYEVVYDTDRQLLPRDMEQIRARMPSSFKITE"
BASE COUNT          339 a          657 c          660 g          357 t
ORIGIN      
        1 cagagcgggg gcgggttgga gagtttgaac ttgatccgca tcagtaacga ggccgcaacc
       61 gcctggtctg ctaccgcagc cggccggaag cggcaggccg tcaggacata gttcacggcg
      121 gcctcatcca ggctttggac gcccgacgtg tcgtaaactg ccgcgctgtc ggcgcacccg
      181 gcggcagaaa cggccacctc gaccaccacc gatccctcga agtaatcgtg cttcgcggcc
      241 accgggtaga actcctccag gtcacccttc gacccgagga ccttgacctg cgaattgccg
      301 ggagctggca gcgtggcttc cggacagggc gtggcgcgca ctcgcgcgag cggcggctta
      361 tgcgcctcgc gctcgtgacg gccgtagacc agcgcgagcc cggctcgttg gtcgttgtaa
      421 gcgcgcagca cacgctgcgc ggtgtcctcc agctgctgca gggcagtcgc ctgcttctga
      481 agatcaccgt ccgttagggc gatgccggcg gtcagctgcc ctgcgaatgt gcggacctct
      541 gcgtctaggg gcgggcgagc agcggccgtt tcatcctgag acagcagtgc cgcaagtttg
      601 tcgaagttcg tcgcgtgcgc accgatacgg ttctgccagc cccagtacag gcccagcaga
      661 aagacgcggt aggtctcgac ctcgaagagc gtggcggcct cgctcagagt cgtacgcatc
      721 cgggcctggt catggcccgc ttcctgcacc ctggcctgcg actccagtgc cgcgatcttt
      781 tgcgcgcggg cgggcgttag cacccactca tcgaggtcgt cccgcacggt gcgggcgcga
      841 acgtccgtgc cggcgaagta tgcccctgag tacagttccc agatgccgtg gtgcagctcc
      901 tgggtggccg catccagcgc ctgcggggat tcaaatttgc gtaactgcgg ccggaatttg
      961 ggatcggcgt tcggcccgaa taagtctgcc gcgagcgcgg gacctaggat catcagaccg
     1021 aggctcaaca gcaggcgctg gccggtgcgg acgcgacgca cgctccccta ctcggtgatc
     1081 ttgaagctgc tcggcatccg cgcccggatc tgctccatgt cgcgcggcag cagctggcga
     1141 tcggtgtcgt acacgacttc gtacagcgga cccgcaccgc cggctgcgcc gggcccggct
     1201 gcgaccgtcc gaatccagaa ctcggtcagc gagtcatcgt gctgcagcgt accggcgaac
     1261 tcgccgttgc gctggctcat gtgctcccag cgatcccgcc gttccttggt ggcgtcgtcc
     1321 gcgggcttgg actggcggta cagagcaaga tggctcttct tgccgcgttc gggacgcagc
     1381 acgacgccgg cgatgtccca ctgggatttg ccacgatcca cgaggtaatt catctgcaga
     1441 tcgagcgagc tcttggcatc gaggttgacg aggccggtag tgtccaattg cagtcgcgcg
     1501 gactcaaggt gcagcggtcc gcccggggaa tactgcagtt tgacgcgttc gaacgcgtct
     1561 ggcatcagat cccggcgcgc caaaaacgcc tgccactgcg caagtgtccc ccggtaggtg
     1621 agatagaagt aatcggcgat gaacttgatc tgctcagcct gcagatcctg gagcccgccc
     1681 ggtacgacac tcgccatgcc gacgtaaccg tccggtgtcg gcaaagcgat cgccagcagg
     1741 gacacatccg cgaagccgag cgaccagatg cgcagctgcc atgtgcgccc gtagtgatcg
     1801 tgcaccagcg catcctgctg ggcggccccc agcgaggtga tgcgtaccgc ctgggtccca
     1861 acctgccggg gaaggttgat gcctttcagc agcagatcga ggaattcctt ggagtcgctg
     1921 tagcggtggg cagccggtat gtcctccgga taggtgagac ggaagagcgt cacccccggt
     1981 ccgccgtcct tgcagtacca gatctcgccg tca
//
LOCUS       DATMRM010000251         5314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1384449, whole genome shotgun sequence.
ACCESSION   DATMRM010000251 DATMRM010000000
VERSION     DATMRM010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384449"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /gene="cysD"
                     /locus_tag="VNZ06_07300"
     CDS             complement(<1..844)
                     /gene="cysD"
                     /locus_tag="VNZ06_07300"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004694369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HWW20595.1"
                     /translation="MHTPEVKLTHLQRLEAESIRIMREVVAECEKPVMLYSIGKDSAT
                     MLHIARKAFYPAPLPFPLLHVDTTWKFKDMYALRDKVAAEPGIRLIVYQNPEAKERGI
                     NPFDHGSQIHTDLWKTQGLKQALDKYGFDAAFGGARRDEEKSRSKERIFSFRSDQHRW
                     DPKNQRPELWSLYNARKHKGESIRVFPLSNWTELDIWQYIYLEDIPIVSLYLAAERPV
                     VERDGTLIMVDDDRFPLKPDEVPQKRMVRFRTLGCYPLSGAIESQATTLPAVIQEMLL
                     ARTSE"
     gene            1011..1775
                     /gene="cysQ"
                     /locus_tag="VNZ06_07305"
     CDS             1011..1775
                     /gene="cysQ"
                     /locus_tag="VNZ06_07305"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HWW20596.1"
                     /translation="MRRSADTLLDQVIGTARTAGEAVMEVYGTDHAVIAKTDKSPVTE
                     ADVRAEAIIISALRNLTPDVPIVAEESVAAGTVPSVGASFWLVDPLDGTKEFIGRNGE
                     FTVNIAMVREGVPVLGVVFAPALGRLFAGVQDGGAFVEDAAGRRHIRCRAVPPEGLTV
                     VASRSHGDAAALKDFLSGRKVEALISAGSSLKLCLVAAGEADLYPRLGRTMEWDIAAG
                     HAVLLAAGGCVTDIAGRPLRYGKPEFENPHFIARGS"
     gene            complement(1856..4369)
                     /locus_tag="VNZ06_07310"
     CDS             complement(1856..4369)
                     /locus_tag="VNZ06_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HWW20597.1"
                     /translation="MSSGSTWIRAFAWLAASVAISAYAAAPPVLQRGYDANVSGANLV
                     ETVLNTSNVGPNTFGLLFNLPVDERIFAQPLYVPNVAIPGQGTHNVLYAATMNDSVYA
                     FDADAGGAALWSINLAGLFGTSAPVWNNFAINSVTVKGNLGILSTPVIDPATNIMYVV
                     ACTLENGTMAYRLYALDITSGAEPYGPGVLITGTYGGATFDARYMIQRASLVLSGNAV
                     VLAFSSIQNEEAGNYVGFVMAYNKLTLQQSGIFATTPTTSGGGVWQSGRPPAVDSAGY
                     VYLFTGNAFGNGYDGVNNFSESVLKFDTSQGLKLVDWFTAGNWSVLDTRDQDLSSSGP
                     ILIPGTTLLAGGGKTGNLYVLNTTSLGKWVANDSQVVQKENITSGLQIHGGPVYWNRS
                     AVNGGPLMYDWAVADVLRAFPFNGSTFATSPSAVGTNNAMAFGGMLALSANAGQQGSG
                     LLWATTVITNTGTGVLHAYDANNIRTELWNSMMSPTRDGYGALGKWVPPVVVNGKVYV
                     PTFSNRVAVYGLGVAPPTFTVTPTLLPFGNVSANGSSTAQAVTVTNTGSAAVPITSIT
                     FSGANPAQFSQTNNCGTSVAIGSACTINVVLTPAGVGSQTAWLNVNAGSGLGTKTSAL
                     SGTGVPPVFSLSPTSVAFGNVQTGSPSAQQLVTVNNGSGAPLPITSITFTGAVPSAFS
                     QTNTCGTPIPTGSTCTIGIVFTPTLAAYEWATLNVSSGSGATENATVNGTGIVTYTLT
                     PATLAFGTTKANTSSAPQSFTVTNTGNAALSVTSISFAGANPTEFSYTTTCGQSVAVG
                     SACTISVVFTPTSIGYAWAKLKVTGSRVTQTTLVNGTGN"
     gene            complement(4568..>5314)
                     /locus_tag="VNZ06_07315"
     CDS             complement(4568..>5314)
                     /locus_tag="VNZ06_07315"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HWW20598.1"
                     /translation="KREDGIAIIDANRNQLERVLRDSSDPEQVALSVNGESLYVASED
                     TGKGIAIDVATGRVRGSVEVGGQPEGVAVHPRRNIVYFTSEVGNSVAVSDATRLRVLS
                     TVAVGQRPRDIAVAPDGSRAYVSGESDASLVVLDGNTDAVITKLTVPGGGARPKGVVL
                     SSDGKRLYVSTGRGGEIVAYDTQSMSVLGFVKVGARPWGLALSPDGHTLYVANGPSDD
                     VSVVTTDPLHVEATVKVGKRPWGVAVLPVK"
BASE COUNT         1017 a         1582 c         1615 g         1100 t
ORIGIN      
        1 gctcagatgt acgtgccaac agcatctcct ggatcaccgc tggaagagtt gttgcctgtg
       61 actcgatggc gccgctcagc gggtagcatc ccagggtgcg gaatcgcacc atgcgcttct
      121 gcggcacttc atcgggcttg agcggaaatc gatcgtcatc gaccatgatc agtgtaccgt
      181 cacgctcgac caccgggcgc tcggctgcca ggtaaagtga cacgatgggt atgtcctcga
      241 ggtagatgta ttgccagatg tcgagttccg tccaattcga caatggaaat acgcggatcg
      301 actcgccctt gtgcttgcgc gcattgtaca agctccaaag ttcgggccgc tgattcttcg
      361 ggtcccatcg atgctggtcg ctgcgaaagg aaaatatgcg ctccttggaa cgtgattttt
      421 cctcatctcg ccgtgcaccg ccaaatgccg catcgaagcc gtacttgtcg agcgcctgct
      481 tgagtccctg ggtcttccac aggtccgtgt gaatctgcga gccatgatca aagggattga
      541 ttccacgctc cttcgcttcc gggttctgat atacgatcag cctgatgccg ggttccgctg
      601 cgaccttgtc ccgcagcgca tacatgtcct tgaacttcca ggtggtatct acatgcagca
      661 agggaaaggg gagcggcgcc gggtagaacg ccttcctagc aatgtgcagc atcgtggcgc
      721 tgtcctttcc gatggaatac agcatcaccg gtttttcaca ttcggcaacg acttcccgca
      781 tgatgcggat gctctcggcc tccagccgct gcaaatgggt caatttcact tcgggcgtgt
      841 gcatggacgc aactttacca gcgttgcagg cttcgtttta atgcaacgga caaatttgtc
      901 gccaagcatc ttatgtcagg tcaaataaat ataggcggga ttgtggcgcc ggccacatcc
      961 ttctaagctc tggcaatcct agagcggcgc aggggcggtg tgtgaaagga atgcggcgat
     1021 cggctgatac gttgctcgac caagtgatag gcactgcccg cactgcgggt gaggccgtca
     1081 tggaagtata cggaaccgac catgccgtga tagccaagac cgataaatct ccggtgactg
     1141 aagccgatgt gcgcgctgag gctattataa tctccgcatt gcgtaatctg acgccggacg
     1201 taccgatcgt cgccgaagaa tccgttgcgg ccgggacagt gccgagcgtg ggagcaagct
     1261 tctggctggt cgatccgctg gatggaacaa aagagttcat cgggcgcaac ggcgaattca
     1321 ccgtgaacat cgccatggtg cgcgaaggag tgccggtcct cggtgtggtt ttcgccccgg
     1381 cattaggccg tttgtttgcc ggcgtgcagg atggtggcgc cttcgtcgag gatgcagcag
     1441 ggcgtcgcca catacgctgt cgcgccgtgc cgccggaagg actgacagtg gtcgcaagcc
     1501 gctcacatgg cgatgcagcg gcgcttaagg atttcctgtc ggggcgcaag gtcgaggcgc
     1561 tgatcagcgc aggctcttcg ttgaaactat gcctggtcgc agcgggcgaa gcggaccttt
     1621 accctcggct aggccgcacc atggaatggg acatagccgc gggtcacgct gtgctcctcg
     1681 ccgccggggg atgcgtcaca gacatcgctg gaaggccact gcgctatggg aagccggaat
     1741 ttgaaaatcc acattttata gcgcgcggct cctgagtcgg agccacaacg gcaacgacat
     1801 cgggaacttg ccaactgctg tgccttcatt caccacagcc agcgctggtc gccggctagt
     1861 taccggttcc gttgacgagg gtggtttgag tgactctcga tccggtgacc ttcagcttcg
     1921 cccaggcata cccgatagag gtcggcgtga atacgacgct gatcgtgcaa gccgatccga
     1981 ccgcgaccga ttgaccgcag gtcgtggtgt atgagaattc ggtggggttc gcaccggcga
     2041 aactgatgct ggtaacgctc agcgcggcgt tgccggtgtt cgtcactgtg aaggactgtg
     2101 gtgcactgga cgtattggcc ttggttgtgc cgaacgccag ggtcgccggt gttaacgtgt
     2161 aggtcacgat tccggttccg ttgaccgttg cgttttccgt tgcgcctgac ccgctgctga
     2221 cgttgagcgt cgcccactca tacgcggcca aggtcggcgt gaacacgatg ccgatcgtac
     2281 aggtcgatcc ggtcggtatc ggcgttccgc aggtattggt ttgcgagaat gcgctcggaa
     2341 ccgcaccggt gaacgtgatg ctggtaatgg gcagcggcgc gccgctgccg ttgttgaccg
     2401 taaccagctg ctgcgcactc ggactgccgg tctgcacgtt gccaaacgcc acagacgttg
     2461 gcgataacga gaagacaggc ggtacaccgg ttccggatag tgcagaggtc ttcgtcccta
     2521 ggccactacc ggcattgacg ttcagccacg ctgtctgact cccgactccg gccggcgtga
     2581 gcacaacgtt gatcgtgcac gctgatccga tggcgaccga tgtaccgcag ttgttggtct
     2641 gcgaaaactg ggcgggattc gcaccggaga acgtgatgct ggtgatcggc accgccgcgc
     2701 tgccggtgtt agtcaccgtc accgcttgcg ccgtacttga gccgttcgct gacacgttgc
     2761 cgaacggcag cagcgttggc gtcactgtaa aggtgggagg cgcgacgccg agtccataga
     2821 ccgcgaccct attggagaac gtcgggacat aaaccttgcc attgacgacc accgggggca
     2881 cccacttacc taaggctccg tagccatcgc gggtcgggct catcatggaa ttccacaatt
     2941 cagtgcggat gttgttggcg tcataggcat gcagcacgcc ggttcccgta ttagtgatca
     3001 ccgtcgtcgc ccaaagcaat ccgctgcctt gttggccagc gttcgcagat agagcgagca
     3061 tgccgccgaa agccatcgca ttgttcgtac ccacggcact gggactcgtc gcaaatgtgc
     3121 tgccgttgaa cggaaacgcc ctgaggacgt cggccacggc ccagtcgtac attaaggggc
     3181 ctccgttgac tgctgatcga ttccagtaca cggggccgcc atggatctgc aatcccgagg
     3241 tgatgttttc tttctgcaca acctggctgt cattggcgac ccacttgccg aggctggtcg
     3301 tgttcagcac atataggttg ccggtcttgc cgccgccggc gagcagcgtc gtacccggga
     3361 tcaggattgg tccggaactg ctcaaatcct ggtcacgtgt atcgagcacg ctccagttac
     3421 ccgcagtgaa ccagtcgacg agcttcaggc cctgggacgt atcgaacttc agcacgcttt
     3481 cactgaagtt gttgacgcca tcgtagccgt tgccgaacgc gttaccggtg aataaataga
     3541 cgtagcctgc actgtcaaca gccggcggac ggccagactg ccagactcca ccgccactcg
     3601 tggtcggggt ggtcgcaaaa atgccgcttt gctgcagcgt gagcttgttg taggccatca
     3661 cgaagccgac gtagttgccc gcttcctcgt tctgaatact gctgaacgca agcacgaccg
     3721 cgttccctga aagcaccagc gatgcgcgct gtatcatgta gcgcgcgtca aacgtggcgc
     3781 caccgtaggt gcccgtgatc aacacgccag ggccgtacgg ttccgcgccg cttgtaatgt
     3841 cgagcgcgta caggcggtat gccatcgtgc cgttctcgag cgtgcaggcg acgacgtaca
     3901 tgatgttcgt ggccggatcg atgaccggcg tgctgaggat gccgaggttg cctttgacgg
     3961 tgacggaatt gatcgcaaag ttgttccata ccggtgccga tgtaccgaac aacccggcga
     4021 gattgatcga ccacagcgcc gcgccccccg cgtcagcgtc gaacgcgtac accgagtcgt
     4081 tcatcgtcgc ggcatatagg acgttgtgcg tgccttggcc cgggatggcg acattgggta
     4141 cgtacagcgg ctgcgcgaag attcgttcat cgacgggaag gttgaacagc aggccaaacg
     4201 tattgggacc gacgttagat gtgttgagca cagtttcgac gagattcgcg ccggaaacgt
     4261 tggcgtcgta cccacgctgc agcaccggcg gcgcggcggc atacgcactg atcgcgactg
     4321 atgcggcgag ccatgcaaag gcccttatcc acgttgaacc tgagctcatc tccccttctc
     4381 ccctaaatcg cgcgcgtccc ggatgacgag caaccaatat tcttaatgtg attattgcca
     4441 gaactctggg ccggcgtatc gctttgtagg cgcgggggcc cgaaaatcaa acgccgtgca
     4501 cagacaaata tgcgttatgt taaattaaag ccatgggtcg tttgcgcgcc gcaaatatac
     4561 cgatcgacta tttcaccggc aagacggcga cgccccaggg gcgcttgccg accttgacgg
     4621 ttgcctcgac gtgcagcggg tccgtcgtga cgaccgacac gtcatcggat ggaccgttcg
     4681 cgacgtaaag cgtatggccg tccgggctga gcgcgagccc ccagggtcgc gcgccgacct
     4741 tgacgaatcc cagcaccgac atgctctgcg tatcatacgc gacgatctcg ccgccgcgcc
     4801 ccgtcgaaac gtaaagccgc ttgccgtcgc tggacaggac cacgcccttc gggcgggcgc
     4861 caccgccggg taccgtgagc ttcgtgatca ccgcatcggt gttgccgtcc agcacgacga
     4921 gcgatgcgtc gctctctccg ctcacgtacg cacgcgagcc atccggcgcc acggcaatgt
     4981 cgcgcgggcg ctggccgacg gcgacggtgc tgagcacgcg tagccgcgtc gcatcggaca
     5041 ccgcgaccga atttcctact tccgacgtga aataaacgat gttgcgccgc ggatgcacag
     5101 cgacgccctc gggttggccg cccacttcga cgctgccgcg aacgcgcccg gtcgcgacat
     5161 caatcgcaat tcccttgccc gtgtcttcgc tggcaacgta gaggctctct ccattcacac
     5221 tcagcgcgac ctgttccggg tcggaactgt ctcgcagtac gcgctcgagc tggttacggt
     5281 tggcgtcgat gatcgcgatg ccgtcctcgc gctt
//
LOCUS       DATMRM010000252         2943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2928836, whole genome shotgun sequence.
ACCESSION   DATMRM010000252 DATMRM010000000
VERSION     DATMRM010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2928836"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VNZ06_07320"
     CDS             <1..705
                     /locus_tag="VNZ06_07320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWW20599.1"
                     /translation="RRDETGVDQAFARFLQEYEIAQRVHHPNIVRLYELGVADDHAYL
                     AMEYFQHGDLRRRMRSGVSPSEALGFACQIGRALGALHSAGIIHRDLKPGNVMLRADD
                     QIALIDFGLAKHEALELEITDTGLIFGTPHYMSPEQGHGQDMDLRSDIYSLGVMLFEM
                     LAGQKPYTATNPMAIIYMHRNAPVPRLPEPLADLQPLLDRMLAKSPADRFVDALETAR
                     QIESTRTAWLLKGALV"
     gene            702..1304
                     /gene="miaE"
                     /locus_tag="VNZ06_07325"
     CDS             702..1304
                     /gene="miaE"
                     /locus_tag="VNZ06_07325"
                     /inference="COORDINATES: protein motif:HMM:NF017939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HWW20600.1"
                     /translation="MNILAVPTPAAWVDAACERWRELLIDHANCEKKAASSALAMIFA
                     YPEDLALTGALSRLAREELRHFEQVSQMMRQVRVEYVRQRPGRYAAALRAALRQHEPA
                     RKLDLMCSHALIEARSCERFKLLAGRLCEPLAGFYESLQHSEARHYELYVRLAHGAQA
                     GGDRARVHARLAQLAAVEAELATSPDDQLRFHSGPPRGSV"
     gene            complement(1274..2008)
                     /locus_tag="VNZ06_07330"
     CDS             complement(1274..2008)
                     /locus_tag="VNZ06_07330"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HWW20601.1"
                     /translation="MSTLFISDLHLDAAEPAAGAQLMQFLERRAVGADALYILGDLFE
                     TWVGDDDDEPYRNRICRALAALSAWGVPCYVMHGNRDFLFQSGFEARTGARLIADPIV
                     IELYGESVLLTHGDALCTADHSYQRLRGVVRNPRWQRRFLALPLKLRRSLAEQARRGS
                     QRHTRHTAYEIMDVDQPAVCAAMQACGVRTLIHGHTHRPAVHEFQLDGHAVRRIVLGA
                     WHHSATCLEWGPGGFELQTLPRGGPE"
     gene            2050..>2943
                     /gene="gltX"
                     /locus_tag="VNZ06_07335"
     CDS             2050..>2943
                     /gene="gltX"
                     /locus_tag="VNZ06_07335"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWW20602.1"
                     /translation="MFADSVVTRFAPSPSGELHLGNARTALFNFLMACKHGGRMVLRI
                     EDTDTARTQEAYVTALCTDLAWLGMKWEEGPDRPGPHGPYRQSQRGAIYQQHFELLER
                     ERLAYPCYCSPLELDLSRRSQLAAGRPPRYAGTCRDLSPQQRQARIDKGIKPTLRFRV
                     PGGKRIEFDDLVHGPQSFLSDDIDDFIIRREDGTAAFFFSNALDDALMRVTHVLRGQD
                     HLSNTPRQRLLLEALSRTAPLYGHVSLLLGADGTPLSKRHGATSIRELREAGFSSVAL
                     CNHLFRLGHSTPNNALLSLEQM"
BASE COUNT          522 a          928 c          964 g          529 t
ORIGIN      
        1 cgccgcgacg aaaccggagt cgatcaggcg ttcgcacgtt ttctgcagga atacgagatc
       61 gcacagcgcg ttcatcatcc gaacatcgtt cgactgtacg aactcggcgt tgccgatgat
      121 cacgcctacc tggcgatgga atatttccag cacggtgacc tgcgccgccg gatgcgtagc
      181 ggcgtgtcgc cctccgaagc gctcgggttt gcctgtcaga tcgggcgcgc gctgggcgca
      241 ctgcactccg ccggcatcat tcatcgtgat ctcaagcccg gcaatgtgat gctgcgagcc
      301 gacgatcaga tcgccctcat cgatttcggc ctcgccaagc atgaggcgct ggagttggag
      361 atcaccgata ccggactgat attcggtacc ccgcactaca tgagtcctga gcagggtcac
      421 ggccaggata tggatttgcg cagcgatatc tacagcctgg gcgtgatgct gtttgagatg
      481 ctggccggac agaagccgta taccgccacc aacccgatgg cgatcatcta catgcatcgt
      541 aacgccccgg tgccgcgcct gccggaaccg ttggccgatc tgcagccgct gctcgatcgc
      601 atgctcgcca agtcgccggc cgatcgcttt gtcgacgcgc tggagaccgc gcgacagatc
      661 gagtcgaccc gcaccgcgtg gctgctcaaa ggggcgctcg tgtgaacatt cttgcggtgc
      721 cgacccccgc tgcgtgggtc gatgccgcat gcgagaggtg gcgcgagcta ctgatcgatc
      781 acgccaattg cgaaaagaag gccgcgtcga gcgcgctggc gatgatcttt gcgtaccccg
      841 aagacctggc gctcaccggc gcgttgtcgc gactggcgcg cgaggaattg cggcacttcg
      901 agcaagtctc gcaaatgatg cgacaggtgc gggtggagta cgtacgtcag cgaccggggc
      961 gctatgcggc cgcgctgcgc gcggcgctgc gccagcacga gccggcgcgc aagctggatc
     1021 tgatgtgctc gcatgcgctc atcgaggcgc gttcctgcga gcgcttcaag ctgcttgccg
     1081 ggcgcctgtg cgaaccgctc gccgggttct atgaatcgct gcagcattcc gaggcgcggc
     1141 actacgaact ctacgtgcgg ctcgcgcatg gggcgcaggc cggaggcgat cgcgcgcggg
     1201 tgcacgcgcg actggcgcag ctggcggcag tggaagcgga attggcgacc agcccggatg
     1261 atcagttgcg atttcactcc gggccgccgc gcggcagtgt ctgaagctcg aatccgcccg
     1321 gtccccattc caggcaagtt gcgctgtgat gccaggcgcc gaggacgatc cgacgcaccg
     1381 cgtgtccgtc gagttggaac tcgtggactg cgggtcgatg cgtgtggcca tggatcaggg
     1441 tgcgaacacc gcaggcctgc atggctgcgc agaccgcggg ttgatccaca tccatgattt
     1501 cgtaggccgt gtgccttgta tggcgttgac tgccgcgtcg cgcctgctcg gcgaggctgc
     1561 gtcgaagttt cagcggcaat gccaggaagc gccgctgcca gcgcggattg cgcaccacgc
     1621 cgcgcaggcg ttgataggaa tgatcggcgg tgcacagcgc atcgccatgc gtgagcagca
     1681 ccgactcgcc gtacagctcg atcacgatcg ggtcggcgat gaggcgcgca ccggtacggg
     1741 cctcgaagcc gctttgaaac agaaaatcgc ggttaccatg catcacgtag cacggcacac
     1801 cccaggcgct caacgccgcc agggcgcggc agatgcggtt gcggtacggc tcgtcgtcgt
     1861 catcgccgac ccaggtctcg aacagatcgc ccaggatata gagcgcatcg gcgccgaccg
     1921 ctcgccgctc cagaaactgc atgagttgcg cgccggcggc cggttcggct gcatccagat
     1981 gcagatcgga gatgaacagc gtgctcaggg cggatcgctc ggcgcttgca gttaggctac
     2041 attgtacgga tgtttgccga tagcgtcgtc acacgctttg cgccaagccc gagcggtgag
     2101 ctgcacctgg gcaatgcacg cacggcgctg ttcaattttc tgatggcgtg caagcacggc
     2161 ggccgcatgg tgctgcgcat agaggacacg gacaccgcgc gcacgcagga agcctatgtc
     2221 acggcactgt gtaccgatct ggcgtggctg ggtatgaagt gggaggaggg accggataga
     2281 cccgggccgc atggcccgta tcgccagtca cagcgcggcg ccatctacca gcagcacttc
     2341 gagctcctcg agcgcgagcg cctcgcctat ccgtgttact gcagtccgct ggagttggat
     2401 ttgtcgcgcc gctcgcagct cgccgccgga cggccgccgc gttacgcggg tacctgtcga
     2461 gacctctccc cgcaacagcg tcaggcgagg atcgacaaag gcatcaagcc gacgctacgt
     2521 tttcgcgtgc ccggcggcaa acgcatcgaa ttcgacgatc tggtgcacgg gccgcagagt
     2581 tttctatccg atgacattga cgatttcatc attcgacgcg aagacggaac cgcagcgttt
     2641 ttcttcagca acgcgctgga cgatgcgctg atgcgcgtga cgcacgtgct gcgcggccag
     2701 gaccatctga gcaacacgcc gcggcagcgg ctgctgctgg aggctttgag caggacggcg
     2761 ccgctctacg gccatgtctc gctactgctc ggcgccgacg gcacgccgtt gtccaaacgc
     2821 catggcgcga ccagcattcg cgagctgcgc gaagctggct tttccagcgt ggcgctgtgc
     2881 aatcacctgt tccgattggg gcactcgacg ccgaataatg ccctgctgtc gcttgagcag
     2941 atg
//
LOCUS       DATMRM010000253         3702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1384563, whole genome shotgun sequence.
ACCESSION   DATMRM010000253 DATMRM010000000
VERSION     DATMRM010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384563"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VNZ06_07340"
     CDS             complement(<1..1002)
                     /locus_tag="VNZ06_07340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWW20603.1"
                     /translation="MSALRVRRWTTEEWLANQAPWGALQARSSADRLFLSCEWLTLWW
                     RYYGSTLGTPDILAFYRGADLVGLVPLYHRRVRRGGLLLAHSVQVIGLSWRDPEPLIS
                     EYLDVIAAREDLDEVRHACAQALLEQPSWSELVIGFTASADRWRAAFAAPVSSKERYV
                     RTLDCCVSYQADLSDGFGAYLLALGQSTRRSLWNLRRRLASLGEVRVEQLRANEIDEG
                     FEDLNRLHALRWGRPAFTGTRLSFHTSLARLLVARGELAFSRLSIGGEVVSVLYDIRR
                     EQRQYNLKMGFDPAFSLRLSLGLIHLGYAMEAAAAEQISVYDFLAGPGRTSDYKRHLS
                     "
     gene            complement(1034..2125)
                     /locus_tag="VNZ06_07345"
     CDS             complement(1034..2125)
                     /locus_tag="VNZ06_07345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWW20604.1"
                     /translation="MSGQIVNTDPSGSRDSGRRASSGFSVAVTSSQGFEREILRLRNA
                     NRDRAETSQYVNWRYQHLAGAPPPQVFWLLAPQGERIGMASVVFRPDWLNGARVQTAV
                     VGDISLDVRWRGQGLGRLLLQEMTAYLDQHFPQHPGFVIPTEAARHALARAGWTAPGT
                     LVPHVYVVDVTRYVGAIIRSAWLTALIARPLRWLARTLARLRAPRDAALILTDAPGEA
                     LQEFLRSVAPSEGAVHDLGAQTLRWRYQQHPNTHFVFATYTRSAEVRGFLVFEETSLE
                     GVCHVYDLAARAPADMRAMLALLILRSLDTPGMITVRMLTDDRHPARRTLRALGFSAR
                     RADAVYQVYSRDGSAERCAWHVTQGDKDT"
     gene            complement(2100..3278)
                     /locus_tag="VNZ06_07350"
     CDS             complement(2100..3278)
                     /locus_tag="VNZ06_07350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW20605.1"
                     /translation="MAKGINILFLIDWFHRTGGTEKHLVQLIAGLPADRFRCTVVAFD
                     MGVNPLLDELRARDVPVIHLPVGREYVPNAAVQAWRLSRLVRRNRYDIVQTFHQKADT
                     FGALIVWLSGARRLVSSKRDTGDLRNPLHVFLNRRLKSLFQAFIVVAEAVRVAVVARD
                     ELPNSRVTTIYNGVDLRQFSVPAAQQRFEARTRFGFGAGDFVVGMVAGFRPEKNHDVF
                     FAGLSRALPKIPALKVLAVGGGELLGQFQEKIWHSELAQRVVFTGELLHVLPCLWSMD
                     VACLTPGSNEGFSNALIEQMAVGLPLIVTDVGGNAEAVIDGVNGYVVPAGDASALAAA
                     LIKTYESREGQRAMGHASRQRAEERFSLDRMCAEHARLYHALCPARQPVNGERADREY
                     "
BASE COUNT          716 a         1215 c         1139 g          632 t
ORIGIN      
        1 gctcagatgg cgcttgtagt cgctggtgcg accaggtccg gcaagaaagt cgtagacact
       61 aatctgctcg gccgcggccg cctccattgc gtagcccagg tgaatgagtc cgagcgacaa
      121 ccgcaagctg aacgcgggat caaagcccat cttcagattg tattgccgct gctctctgcg
      181 gatgtcatag agcaccgaga ccacttcacc gccaatactc aggcgcgaaa acgcgagctc
      241 tccgcgcgcc accagcagcc tcgcgagact cgtatgaaat gacagtcgcg tgccggtgaa
      301 cgccggccgc ccccagcgca gagcgtgtag cctgttcaga tcctcgaagc cctcgtcgat
      361 ctcattcgcg cgtaactgct cgacgcgcac ctcgcctagg ctagcgaggc gacgacgcag
      421 gttccagaga ctgcggcgcg tcgactgccc cagcgcgagc aggtacgcgc caaagccatc
      481 gctaagatct gcctggtagc tgacgcagca atccagggtg cgcacgtagc gctccttgct
      541 cgacaccggt gccgcaaacg cagcacgcca gcggtcggcg gacgcggtga agccaatgac
      601 gagctcgctc caagacggct gttcaagcag cgcctgcgcg cacgcgtgtc gcacttcgtc
      661 cagatcctcg cgcgcggcaa tgacgtcgag gtactctgaa atcaacggtt ccggatcgcg
      721 ccacgacaac ccgataacct gtactgaatg cgccaacagc aggccgccgc gcctgactcg
      781 gcggtgatac aggggaacca gacccacaag gtcggcgccg cggtagaacg ccaggatatc
      841 gggagtgccc agcgtacttc catagtagcg ccaccagagc gtcagccatt cgcaggacag
      901 aaacagccga tcagcgctcg aacgcgcctg cagcgcaccc cacggggcct gattggccag
      961 ccactcctcg gtcgtccagc ggcgcacgcg cagcgcgctc atcgtcccct cctcgctgcc
     1021 gaagggcgcg ccatcaggtg tccttgtctc cctgggttac gtgccaggcg caccgctcgg
     1081 cgctgccgtc gcgcgaatac acttgataca cagcgtccgc acggcgtgcg ctgaacccga
     1141 gggctcgcaa agtcctcctc gcggggtgac ggtcgtcggt cagcatgcgc acggtgatca
     1201 ttccgggggt gtccagactg cgaagtatga gaagcgccag catcgcgcgc atatcggccg
     1261 gtgcgcgcgc ggccaggtcg tagacgtggc agaccccctc gagcgaggtt tcctcgaaga
     1321 ccagaaaacc gcgcacttca gcgctgcgcg tgtaagttgc aaaaacgaaa tgagtgttgg
     1381 ggtgctgctg atagcgccat ctgagcgtct gggcgccgag gtcgtgcacc gcgccctcgc
     1441 ttggggcgac gctgcgcaga aattcctgca gagcctcacc gggcgcgtcc gtcagaataa
     1501 gtgcggcgtc gcgcggcgcg cgcaggcgcg cgagtgtgcg cgcgagccac cgcagcgggc
     1561 gcgctatcaa ggcggttaac caagcgctgc ggatgatcgc acccacataa cgcgtgacat
     1621 ccaccacgta gacgtgcggt acgagcgtgc cgggcgctgt ccagccggcc ctggcgagcg
     1681 cgtggcgcgc agcctcggtt ggaattacaa agccggggtg ttgaggaaag tgctggtcga
     1741 ggtaggctgt catctcctgc agcagcagcc ggcccagccc ctgtccgcgc cagcggacat
     1801 cgagtgagat gtcacccacg acggcagtct gtacgcgtgc tccattgagc cagtcgggtc
     1861 gaaacacgac cgacgccata ccaatgcgct ccccctgcgg ggcgagcagc cagaacacct
     1921 gcggcggtgg cgcacccgcc aaatgctgat aacgccagtt gacgtattgc gacgtttcgg
     1981 ctcggtcgcg gttggcgttt cgcaatcgca gtatttcccg ctcgaagccc tgcgaggagg
     2041 ttaccgcgac ggaaaatccc gagctggcgc gcctgcccga atcccgggag ccactcgggt
     2101 cagtattcac gatctgcccg ctcaccgttc acaggctgcc gggccgggca cagcgcgtgg
     2161 tagagtcgcg cgtgttctgc gcacatccgg tcgagcgaaa atcgctcctc cgcacgctgg
     2221 cgcgaagcgt gtcccatggc gcgctggcct tcgcgactct catacgtctt gatcagcgcg
     2281 gccgccaggg cacttgcgtc acccgcggga acgacgtagc cgttgacacc gtcgataacc
     2341 gcttccgcgt taccgccaac gtcagtgacg atcagcggca gacccacggc catctgttcg
     2401 atcaacgcgt tggagaaccc ctcgttgcta cccggcgtca ggcaggcgac atccatgctc
     2461 cagaggcacg gcaacacgtg gaggagctcg ccggtaaaca cgacgcgctg cgcaagctcg
     2521 ctgtgccaga tcttctcctg gaattggccc agcagttcgc cgccccccac ggccaagacc
     2581 ttcagcgcgg gaatcttagg cagcgctcgc gataaacccg cgaaaaacac gtcatggttt
     2641 ttttccggcc ggaaacccgc caccataccg accacaaaat cccccgcgcc gaacccaaag
     2701 cgcgtgcggg cctcgaaccg ttgctgcgca gcaggaacac tgaattgcct caggtcaacc
     2761 ccattgtaga tcgtcgttac ccgtgagttc ggcagttcat cgcgcgccac cacggccact
     2821 cgtacggcct ctgccactac aatgaacgcc tgaaagagcg acttgaggcg acgattgaga
     2881 aaaacatgca gcgggttgcg caggtcgccg gtgtcgcgct tgctcgatac caagcgcctg
     2941 gcgccggaga gccacacgat gagcgccccg aacgtgtcgg ctttctggtg aaaggtctgc
     3001 acaatgtcat atctgttgcg ccgtaccagc cgcgacaaac gccacgcctg cactgccgcg
     3061 ttgggcacgt actcgcgtcc gaccggcagg tggatgactg gaacatcgcg ggcccgaagt
     3121 tcgtccagca gcgggttgac acccatgtcg aaggcaacga cggtacagcg aaagcgatcc
     3181 gcgggcagcc ctgcgatcag ttgcacgaga tgtttttcgg taccgccggt gcggtgaaac
     3241 cagtcgatca gaaagagaat attgatgccc ttagccattc atacatcgcc ggatcgccaa
     3301 cgcgcgcggc cagtatatcc atctcctgag gctgcccccg cgcgacggca ctcaccctgc
     3361 aatctcaata attgttagtc acatcaatct cttacagagg caaaagggac tcggcgcccg
     3421 ggccgacgtg tccccgtgtc gctgggatag ggtgcacggt ggctttggcg ccgcggcgct
     3481 attctttgtc gggatcgtcc gcgtctttgc cgggtgcgtg tggcggactg cgcagccggc
     3541 gccgctagca gcgaggatca acaaggtttg atgcgcaaga acgcggcaag gacgtttcga
     3601 cccgatatcg agggtctgcg tggcatcgcg gtctttgtgg tggtcgcatt tcactgcgga
     3661 cttccgggtt tctccggagg tttcatcggg gtcgatgtct tc
//
LOCUS       DATMRM010000254         9178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1715256, whole genome shotgun sequence.
ACCESSION   DATMRM010000254 DATMRM010000000
VERSION     DATMRM010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9178
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715256"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VNZ06_07355"
     CDS             <1..550
                     /locus_tag="VNZ06_07355"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HWW20606.1"
                     /translation="DARVAFASLRYPLALATTGLSAVAIALAIWWWMHRRWERAGQGS
                     TQPPVPGSGWFSGLRAVHHEVMRRGPGMMLHAAIPACVQVLLSGIATAVLAKSLGIDL
                     ALLTAMWVTAAVYAVVLLPISVAGVGVREITLTRSLALLGLDSSLAVALSILLFADPI
                     INALIGGALQMRAAFAEVQRQS"
     gene            complement(541..1944)
                     /gene="gltX"
                     /locus_tag="VNZ06_07360"
     CDS             complement(541..1944)
                     /gene="gltX"
                     /locus_tag="VNZ06_07360"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWW20607.1"
                     /translation="MTVVTRFAPSPTGMLHIGGVRTALFSWLYARHFGGKFILRVEDT
                     DRERSTDEAVRVILDGMAWLDLRADEGPFFQTQHYGRYRTVIQQMLENGNAYHCYCTK
                     QELDQMREQQLAHKEKPRYDGRCRERREPRTGVTPVVRFKNPLEGDVVVEDLVHGTVV
                     FKNAELDDLIIERSDGNPTYNFCVVVDDWDMGITHVIRGDDHLNNTPRQMNMLRALGA
                     VPPAYAHVPMILGADGAKLSKRHGAVSVLQYQEEGFLPDALLNYLVRLGWSHGDQEVF
                     SREEMIAAFDIKDVNKAASAFNPDKLLWLNQQHLMRAPVSTLLAGLRSQLAMLGIQAQ
                     GDLLLEGVVNAQRERAKTLKEMAHNSVFFFREFEHYDERAAKKNLNHEAVPLLRALQA
                     GFNTVAKWDAGTIHEVISSVATRYGLGLGKVAQPLRVAIAGGPVSPPIDLTVALLGAE
                     RTGARIERALNWIGNQL"
     gene            2038..2112
                     /locus_tag="VNZ06_07365"
     tRNA            2038..2112
                     /locus_tag="VNZ06_07365"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2070..2072,aa:Val,seq:cac)
     gene            complement(2152..3249)
                     /locus_tag="VNZ06_07370"
     CDS             complement(2152..3249)
                     /locus_tag="VNZ06_07370"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWW20608.1"
                     /translation="MSVRPQIQTDFKINNFDLLRLIAATQVVLIHTERHLDMVRPAWW
                     PLVDALPGVPIFFVISGFLISASYERSSTLSSYARNRILRIFPGLWCCILLTIGVAAA
                     FGFSFAHWSAGSWLLAQLIGLIYTPHFLKSFGFGSYNGSLWTIPIELQFYLVLPIFYY
                     LTKRAGNQTKGLSLALLLFALIAFLANLLFEPLTEVTQEPIAQKLIRYSFFPHIYLFL
                     SGVILQRLSVHRSRWIAGKALHWLAVYLLFHFAVPYSAVSYVIGTMLLGVVVIAAAYT
                     RPTLSEHLLRGNDISYGVYIYHGLIINIFVEFGLGGRESDLAVVVLLAFLLGYLSWLL
                     IERPFLRRKKQTIHVLASPAGLHVTSVGKGP"
     gene            3553..4602
                     /locus_tag="VNZ06_07375"
     CDS             3553..4602
                     /locus_tag="VNZ06_07375"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWW20609.1"
                     /translation="MSRIKTLRVQELDALRLLAALSVVGFHYGINFHFWPSAEGALKY
                     GFLGVELFFMISGFVILQTATRKSAYEFMCSRFARLYPTFWCVLLLTWPMRGGGTPIQ
                     ALANATMAARLLHQPVFDPVYWTLVIELKFYILILALISLRQIQRIELWLIAWLGACV
                     ASLVTGHFRALTFDLYAPYFTAGCVLYLIYVQGLTLTRALTISACLVLAFTDAKIVAD
                     KYDGIVVSYVTVKLIVSALFVVFLSVAMRWIKLPNWKVWSSLGLLTYPIYLVHSVPGF
                     TVWSWLPKTMSPQLRTLCVCAFVFSVSGLLAATIERKGCSMLYSALVRIPDRIIAWIR
                     GPVAPPTQKAPLVEP"
     gene            complement(4588..6117)
                     /locus_tag="VNZ06_07380"
     CDS             complement(4588..6117)
                     /locus_tag="VNZ06_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20610.1"
                     /translation="MFDPNGIEAERLIAQRRLDLARSQTERNLRGQFATPAALADDML
                     CYAREHFGTGAVRFLDPAIGTGSFYSALLRTWSGSNITRATGFELDPDYGRASRALWS
                     GSRLRLRIEDFTAASAPRSEARRYNLIICNPPYVRHHHMTSANKQRLHRQARTASGST
                     LSGLAGLHAYFLMLTHPWMSSGALAGWLIPSEFREVNYGDAIRDYLLRRVTLLRVHCF
                     EPSDLQFNDALVSSAIVWFRNRPPSVDHTVELTCDGPLSRPRACRQVSARELSEVRKW
                     RSRATTAVNDPVLGEFFKIQRGLATGANRFFIMTAAEARERGIPAAYLRPILPGPKQL
                     AADEILADERGYPRLPCRLVIVDCPLPLAQVRARHPAFWRYLQSGLGGVSAGYLCSRR
                     SPWYSQESRQSTAFLCSYFARWRGERLHRFIFNHSRAIAANNYLMLYPRGELLQFIGG
                     NLTRARAVWRELCNLKAESLRYAGRSYGGGMYKLEPKELASVPAAPIGALLRTGSSGL
                     D"
     gene            6134..6730
                     /locus_tag="VNZ06_07385"
     CDS             6134..6730
                     /locus_tag="VNZ06_07385"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWW20611.1"
                     /translation="MIMFRLARHVALATTLACLVLLGGCVTTTTTTPAVGSPTKTITT
                     ATPTKAKAAQPKPADTSGANAVSIEASNALLATATRQVIAAEEAFAKTMADRNFKSFV
                     TFLSQDAVFFSGTEVSRGPAAVAAKWETYFSGREAPFSWTPDHVEVLASGNLALSTGW
                     VYQNHKPVGRFNSIWRLEAANTWRVVFDKGEAICGTTP"
     gene            complement(6727..8355)
                     /gene="murJ"
                     /locus_tag="VNZ06_07390"
     CDS             complement(6727..8355)
                     /gene="murJ"
                     /locus_tag="VNZ06_07390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWW20612.1"
                     /translation="MNGGGAPTVPDDATTRTERLNTRAAGTITLAVLCSRVLGLAREA
                     ICGALFGGAVMDTFTVAFRIPNLLRDLFAEGALSTAFVTTFSKTIARSGDRQAWQLAN
                     KVATLTAVVLSVICLLGIAFSPLLVAVLADGFDAEKAARTVTLTRIMFPFILLVSLAA
                     LVMGMLNSKNVFGMPAMASSFFNIGSIVAGVLLGYWFDPHFGPRALVGFAIGTLIGGA
                     MQLGVQLPSLRGVGYRFHPDFAWRDAGVRAIVLLMGPSVIAASTTQVNVMVNSAFASS
                     LEKGAIFSLAIAFRLMQLPLGLFGVALGTVTLPLLSRLAVAGDMPAFRGELSHGMRLA
                     LLLSIPSTVGLMVLAEPIMSVLYQHGRFDASAATQAGEALRYYAIGLSGYAALKVLVN
                     GFYAVDRRKTPMIISFMAVGLNLIFNWLFTFRLGWGIRGLSFSTGCVATCNFLTLYVL
                     MRRQLKGLETRRMLRLLLKVALASSALAAVCWASRHWLLADWATREFATKTAYLLLTV
                     VLGAITFLGCALALRIEELSELVAAVRRRLQRAR"
     gene            complement(8352..9146)
                     /locus_tag="VNZ06_07395"
     CDS             complement(8352..9146)
                     /locus_tag="VNZ06_07395"
                     /inference="COORDINATES: protein motif:HMM:NF025175.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HWW20613.1"
                     /translation="MPYTVSHVAAVMPGYQLLSRAHVFSAAVIGSMVPDFGLLLPRDP
                     ARVETHSLMALFTFCLPVGLVAYWLTQLLIKPALLEALPDGPYLRLRGSHPAASLVHI
                     KTWFTASGAILFGAFTHILWDEFTHEDTRAGRVFPFLKDYSPDLHGHPVALWRWLQLG
                     SSAVGLTVVLIALGVWIYHARREQVPHTPRRIPADERLMWLCLYLVPPLVAVSWAFWR
                     AYSTGASAFEGTPGYGLGSVAVASVRGAVVSLLFISALLKIRSART"
BASE COUNT         1946 a         2832 c         2703 g         1697 t
ORIGIN      
        1 cgatgcgcgc gtcgccttcg cgagcctgcg ctatccactg gcgctggcta cgaccgggct
       61 gtcggcagtg gcgatcgcac tagcgatctg gtggtggatg catcgtcgct gggagcgtgc
      121 tggtcaaggc tcgacgcagc cgcctgtgcc tggcagcggc tggttctcag gtctgcgcgc
      181 ggttcatcac gaagtcatgc gtcgcggtcc aggaatgatg ctgcatgccg cgattcccgc
      241 ttgcgtccag gtgctgctat cgggtatcgc caccgcggtg ttggccaaat cgctgggcat
      301 cgacctggcg ctgctgactg cgatgtgggt gaccgccgct gtctatgcgg tagtgctgct
      361 gccgatctcc gtggccggtg ttggcgtacg cgagatcacc ctcacccgat ctctggcgct
      421 gctgggattg gactccagtc tcgcggtggc gttgtcgatc ctgttgttcg ccgatccgat
      481 catcaatgcg ctgattggcg gcgcactgca gatgcgcgcc gctttcgccg aggtgcaacg
      541 tcagagctga ttgccgatcc agttcagtgc gcgttcgatg cgagcgcctg tcctttccgc
      601 tccgagcagc gcgacggtga gatcgatggg cggcgacacc ggaccccctg caatcgcaac
      661 gcgcagcggc tgcgccacct ttcccagtcc gaggccatag cgggtggcaa ccgaactgat
      721 cacttcgtgg atcgtgccag catcccattt cgccacggtg ttgaaccctg cttgcagcgc
      781 acgcagtagc ggcacggctt cgtggttcag gttcttcttg gccgccctct cgtcgtagtg
      841 ctcaaattcg cggaagaaga agacactgtt gtgcgccatt tccttgagcg tcttggcccg
      901 ctcacgctgc gcgttcacca cgccttcgag caatagatcg ccctgagcct gtattcccag
      961 catcgcgagc tgggaacgaa gacccgcaag cagtgtactg accggcgcac gcatcaagtg
     1021 ctgttgattc agccatagca gcttgtcggg attgaacgca gaagccgcct tgttgacgtc
     1081 cttgatatcg aacgcagcaa tcatctcctc gcgcgaaaac acttcctgat cgccgtgcga
     1141 ccagcctagc cggaccagat agttcagcag cgcatccggt agaaacccct cctcttggta
     1201 ttgcagcaca ctgaccgcgc catgccgctt tgacagcttg gcaccgtcag cgccaaggat
     1261 cattggaacg tgtgcatagg ccggaggcac agcacccaga gcacgcaaca tattcatctg
     1321 ccggggtgtg ttgttaaggt gatcgtcacc acgaatgacg tgcgtgatcc ccatatccca
     1381 atcgtccacc accacgcaga agttgtaggt cggatttccg tctgaacgct cgatgatgag
     1441 atcatcgagc tccgcgttct tgaacactac cgtaccgtgt accaggtcct cgaccacgac
     1501 atcgccttcg agcggattct tgaagcgcac gaccggggta actccggtgc gtggctcgcg
     1561 acgttcccgg caacgtccgt catatcgggg cttctccttg tgcgcgagct gttgttcgcg
     1621 catctgatca agctcctgct tggtgcagta gcaatgatag gcattgccat tttccagcat
     1681 ctgttggatg acagtccgat agcggccgta gtgctgggtt tgaaagaacg gaccctcatc
     1741 ggcacggagg tccagccatg ccataccgtc gagaatgacc cgcacggcct catcggttga
     1801 acgctcgcga tcggtgtcct cgacgcgcag gatgaacttc ccgccgaagt ggcgcgcata
     1861 cagccatgag aacaacgcgg tgcgcacgcc cccgatgtga agcatgccag taggacttgg
     1921 agcaaagcgg gtaacgacag tcatttatgg actcgcagct tggggagcga ttggaagcct
     1981 cgcataaatt gattcgccac gggcggccgc ttaaactctc cgccccttga gaggcaaggg
     2041 cggttagctc agcggtagag cactgctttc acacggcagg ggtcactggt tcaatcccag
     2101 taccgcccac cattaaaatc agaaacttag agtcgttccg gggtcctcga actaaggacc
     2161 cttcccgacg gacgttacgt gtaagcccgc gggtgaagcc aacacatgta tcgtttgttt
     2221 cttacggcgc agaaacggcc gctcgatcag cagccacgac aggtaaccca ggagaaaagc
     2281 caatagcacc acgaccgcca ggtcgctttc ccgtcctcca agcccgaact ccacgaagat
     2341 attgatgatc aaaccatgat agatataaac accgtaggag atatcgttac cccgcaacaa
     2401 gtgttcggac agcgtcggcc tggtgtaagc cgccgcgatc accacgacac ctaacagcat
     2461 cgtgcctatc acgtagctca cggcggaata cggcacagcg aaatggaaca gaagatagac
     2521 ggccagccaa tgcagcgcct ttcctgcaat ccaacgggag cgatggaccg atagccgttg
     2581 caggatcacg ccgcttagaa acagatagat gtgcggaaag aaagaatatc gaatcaactt
     2641 ctgcgcaatc ggctcctgcg tgacctcagt gagcggctca aaaagcagat tggccagaaa
     2701 tgcgatcaac gcgaaaagca acagcgccag gctgagcccc ttggtctgat ttcccgcgcg
     2761 cttggtgaga tagtagaaga tcggcaatac cagatagaac tgcagctcta tcgggatagt
     2821 ccacagactg ccgttgtatg agccaaagcc aaagctcttc agaaaatgcg gggtataaat
     2881 gagcccaatc agttgggcca gcaaccaact ccccgccgac cagtgcgcga acgaaaagcc
     2941 gaacgcggcc gccaccccga tcgtgagcag tatgcagcac cacaggcccg gaaaaattct
     3001 gaggatccgg ttccgggcat agctgctgag cgtcgagcta cgctcgtatg acgccgaaat
     3061 caggaagcca cttatgacaa agaaaattgg aacaccgggc aaggcgtcca cgagcggcca
     3121 ccatgccggt cgaaccatat ccagatgccg ctcggtatgg atcagaacga cctgagtagc
     3181 ggcaatgagc ctcaacaggt cgaaattatt gatcttgaag tcggtttgga tttggggtcg
     3241 tacgctcaag acaagtcgac tcgggccaaa atgccggcga aatcatgccg catatttgca
     3301 gcattggcat cgctttcctg acgaccctca ccagacgctt gagcaccttt cagcagcgcc
     3361 cggcttgagc ttaccggatg ctcgccagtc gtcgatgggt cgtagcgagt tgatttcact
     3421 tatgtaaaaa taacacatcg gcctcaccat cattttccgg aaaatgaggt tttccggcag
     3481 gcggaacgcg tgcgccgatg tcagtatccc tgtctcaatt tcaggtgcca ctcggggcga
     3541 taagagaatc ttgtgagcag gatcaaaact cttcgagtgc aagagctcga cgcattgcga
     3601 ttgctcgccg ccctttccgt tgtgggcttt cactacggca tcaacttcca tttctggccg
     3661 agcgctgagg gcgcgctgaa atacggcttt cttggggtcg agctgttctt catgatctcg
     3721 ggcttcgtga tattgcagac ggcaacgcgc aagagcgcct atgagttcat gtgctcgcgc
     3781 ttcgcacgac tctatccgac attctggtgc gtgctcctgc tgacatggcc gatgagaggc
     3841 ggaggaaccc cgattcaggc gctggccaac gcaaccatgg cggcgaggct gttgcaccag
     3901 ccggtattcg atccggtgta ctggaccctg gtcatcgagc tgaagttcta catactgata
     3961 cttgcactga tatcgctgcg tcagattcag cgcatcgagc tgtggttgat cgcatggttg
     4021 ggagcctgcg tcgcctcgtt ggtgacggga cactttcggg cgctgacatt cgatctctat
     4081 gcgccctact tcactgccgg ttgcgtgctg tacctgattt acgtgcaagg cctgacgttg
     4141 acgcgagccc tgacgatttc tgcatgtctg gttctggcgt tcacagatgc caagattgtg
     4201 gcggacaaat atgacgggat cgtcgtttcc tatgtgaccg tgaagctgat cgtgtcagcg
     4261 ctatttgtgg tctttctgag tgtcgccatg cgatggatca aacttcccaa ctggaaagtg
     4321 tggtcatcgt tgggacttct gacctaccca atttacctgg tccattcagt tccgggcttc
     4381 accgtctggt catggctgcc taagacgatg agccctcaac ttagaactct gtgcgtatgc
     4441 gcgtttgtgt tcagcgtttc cggactactg gccgccacga tcgagagaaa aggctgttcg
     4501 atgctgtatt cggctcttgt gcggataccc gacagaatca ttgcctggat cagaggacct
     4561 gtcgcacccc cgacgcagaa ggctccccta gtcgagccct gagcttcccg tccgcaacaa
     4621 tgcgccaatc ggcgcggctg gcacgcttgc cagttctttg ggctcgagct tgtacatgcc
     4681 accgccatag gaccgaccgg catatcgcaa cgattcggct ttgagattgc acagctcgcg
     4741 ccagactgct cgagcacgtg tcaggtttcc gccgatgaac tgaagcagct cgccgcgcgg
     4801 atagagcatc agatagttgt tggcggcgat cgcccggctg tgattgaaga tgaagcgatg
     4861 cagcctctca ccgcgccaac gcgcgaaata actacagagg aacgcggtgg actgcctgct
     4921 ctcctgcgaa taccaggggg aacgcctgct gcacaggtag cccgccgaca cgcccccgag
     4981 cccgctttga agatagcgcc aaaacgcggg gtgacgcgcc cggacctgtg ccaagggcaa
     5041 tggacagtca acgatcacga gtctgcacgg cagccgcgga tagccccgtt cgtcggccag
     5101 aatctcatcc gctgcaagct gcttcggacc gggcagaatc ggccgcagat aggccgccgg
     5161 tatgccgcgc tccctggcct ctgcggccgt catgatgaag aaacgattcg ctccggtcgc
     5221 caatccgcgc tggatcttaa agaactcccc gagcaccgga tcgttgactg cggtggtggc
     5281 gcgtgatcgc cacttgcgta cctcggacag ctctctggcg gatacctgcc gacacgcgcg
     5341 cgggcgcgag agcgggccat cacaggtcag ctcgaccgtg tgatcgaccg atggagggcg
     5401 attgcgaaac cagacgatgg ccgaactcac cagcgcgtcg ttaaattgca gatcagaggg
     5461 ttcgaagcag tgtacgcgca gcagcgtcac ccgccgcaac aggtagtcac ggatggcatc
     5521 gccatagttg acctcccgga actcgctcgg gatgagccaa cctgccagcg cgccgctact
     5581 catccacgga tgcgtcagca tcaggaagta agcatgaaga cccgccaagc cgctcaacgt
     5641 cgagccgctg gcggtccgag cctgccgatg caggcgctgt ttgttggcgc tcgtcatatg
     5701 gtgatgacgc acgtacggag gattgcaaat aatgaggttg taacgtcgtg cctcgctgcg
     5761 aggcgcggag gccgcggtaa agtcctcgat ccgcagtcgc aagcgcgacc ctgaccacaa
     5821 cgctcgactg gcccgcccat agtctgggtc gagttcgaat cccgtcgcgc gggtgatatt
     5881 cgaaccgctc caggtacgca gcagtgctga gtagaacgat ccggtgccga tcgccggatc
     5941 tagaaacctg accgccccgg taccaaaatg ctcacgtgcg tagcacagca tatcgtccgc
     6001 cagggcggcc ggagtggcaa actgaccgcg caggtttcgt tcagtttgtg agcgcgcgag
     6061 gtccagtcgc cgctgtgcga tcagtcgttc cgcctcgatg ccgttgggat caaacatgcc
     6121 tggaggtcat cccatgatca tgttccgcct cgcgcgccac gtcgctctcg cgacgaccct
     6181 tgcctgcctt gtcctgctcg ggggctgtgt gacgaccacg acgacgacgc ccgcagtcgg
     6241 ttcgcccacc aagaccatca ccaccgcgac ccccaccaaa gccaaagccg cccagcctaa
     6301 gccagcggac acttccggcg cgaatgcggt ctccatcgag gccagcaacg cactgctcgc
     6361 gacggcgacc cgtcaggtca ttgcggccga agaagcgttc gccaagacca tggctgaccg
     6421 caacttcaag tccttcgtga cattcctgtc gcaggatgcc gtgttcttca gtggcaccga
     6481 agtatcgcgc gggccggcag cggtggccgc caaatgggag acgtatttct ccggacgcga
     6541 ggcaccgttc agctggacgc ccgatcacgt cgaggtactc gcttcgggca acctggcgct
     6601 cagcaccggc tgggtatacc agaaccacaa gcctgtcggt cgcttcaact ccatctggcg
     6661 actcgaggcc gccaacacct ggcgagtggt gttcgacaag ggtgaggcga tctgcggcac
     6721 gacaccttag cgcgcgcgct gcaggcgccg ccgaacggca gccaccagct cactgagttc
     6781 ctcgatgcgc agtgcaagcg cgcagcccag aaatgtgatc gcaccgagca ccacggtgag
     6841 cagcagatag gccgtcttgg tcgcgaactc gcgcgttgcc caatccgcca gcagccagtg
     6901 ccgcgaggcc cagcacaccg ctgcgagtgc gctcgaggcc agcgcgactt tcagcagcag
     6961 cctcagcatg cgccgcgttt ccaggccttt gagctggcgc cgcatgagca catatagcgt
     7021 caggaaattg caggtcgcca cgcaaccggt tgaaaaggac agtccgcgta tgccccagcc
     7081 caaccggaac gtgaacagcc agttgaagat caggttcagc ccaaccgcca tgaagctgat
     7141 gatcatcggc gttttacgcc ggtccactgc gtaaaagccg ttcaccagga ccttcagcgc
     7201 cgcgtagcca ctcagaccga tcgcgtagta gcgcagtgcc tcaccggcct gcgtcgcagc
     7261 gctggcatcg aatcggccgt gctgatagag caccgacatg atgggttcgg ccaacaccat
     7321 caatcccact gtcgagggta tcgacaacag caatgccagc cgcatgccat gcgagagctc
     7381 accacggaac gcgggcatgt caccggctac cgccagccgc gatagcaggg gtaaggtcac
     7441 tgtgccgagc gccacgccga acaggccgag cggcaactgc atcaatctga acgcgatcgc
     7501 gagactgaag atcgcgccct tctcgagaga ggaggcgaac gcggaattca ccatcacatt
     7561 gacctgcgtg gtactggcgg cgatcaccga gggacccatc agcagcacaa tcgcgcgaac
     7621 gccggcgtcg cgccacgcga aatccgggtg aaagcggtaa ccgacgccac gcaacgaggg
     7681 cagctgcacc cccaactgca tggcaccgcc aatcagggtg ccgatcgcaa atccgaccag
     7741 agcgcgcggt ccgaaatgcg ggtcgaacca atagcccagc aacacgcccg cgacgatcga
     7801 gccgatgttg aaaaagctgg acgccatcgc cggcatgccg aatacgttct tcgaattgag
     7861 catccccatc accagcgcgg cgagcgacac cagcagaatg aacgggaaca tgatccgggt
     7921 gagcgtcacc gtgcgggccg ctttctcggc atcgaagccg tcggcgagca ccgcgaccaa
     7981 cagcggtgag aaggcgatgc ccagcaggca gatcacgctc aagacaaccg cggtcagcgt
     8041 cgcgaccttg ttggccagct gccaggcctg cctgtcgccg gagcgcgcga tggtcttgct
     8101 gaacgtcgtg acgaaggccg tggacagagc accttcggca aacaggtcgc gcagcagatt
     8161 gggtattcga aaggcgacgg tgaacgtgtc catgacggca ccgccgaaca gggcaccaca
     8221 gatggcctcg cgcgccaggc ccagcacgcg actgcacagc acggccagcg taatcgtgcc
     8281 agccgctcgc gtattcagac gctccgtacg cgttgtcgca tcgtccggaa cggtcggggc
     8341 gccgccgccg ttcatgtcct ggcggatcga attttaagca acgcgctgat gaacaacagc
     8401 gacaccaccg cgccgcggac cgaggccacg gcgacactgc ccaggccgta gccaggtgtc
     8461 ccttcgaagg cggacgcacc ggtgctgtag gcgcgccaga atgcccagga gaccgctacc
     8521 agcggcggca ccaggtacag acacagccac atgagacgct cgtcggcggg aatccggcgc
     8581 ggtgtgtgag gaacctgctc gcggcgggcg tgatagatcc acactcccag tgcgatcaac
     8641 accaccgtga gtccgacagc gctgcttccc agctgcaacc agcgccacag cgcgaccgga
     8701 tgtccatgca gatcagggct atagtccttc agaaacggga aaacccggcc ggctcgcgta
     8761 tcctcatgcg tgaactcatc ccacaggatg tgagtgaatg cgccgaatag aatcgctccg
     8821 gacgccgtaa accaggtctt gatgtgcacc agcgacgccg ccgggtgcga gccgcgcaat
     8881 cgcaggtacg gaccatcggg tagagcctcg agcaatgcgg gcttgatgag cagctgcgtg
     8941 agccagtagg caaccagacc cactggcagg cagaaggtaa acagtgccat caagctgtgg
     9001 gtttcgacac gcgcgggatc gcgcggcaac agcaggccaa aatcaggcac catgctgccg
     9061 atgaccgcgg cggagaaaac gtgcgcacgc gacagcagct ggtagccggg catgactgcc
     9121 gcgacgtgag agacagtgta gggcacggtt cagccgcccc agtcgcgaac gtgcgcat
//
LOCUS       DATMRM010000255        20006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4173512, whole genome shotgun sequence.
ACCESSION   DATMRM010000255 DATMRM010000000
VERSION     DATMRM010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 20006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20006
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4173512"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..781
                     /locus_tag="VNZ06_07400"
     CDS             101..781
                     /locus_tag="VNZ06_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWW20614.1"
                     /translation="MKVLLVEDNERVTQFVVKGLQEAGHTADHAANGRDGMFLAASEP
                     YDVIVMDRMLPGDIDGLAIIEALRKSGKRTPILILSALAEVDERIKGLRAGGDDYLTK
                     PFAFGELLARLDALTRRAQGENAQTRLEVADLRVDLLTRKVTRGNRGITLQPREFKLL
                     EYLMRHADQVVTRTMLLENVWDYHFDPQTNVVDVHISKLRQKIDADFERPLLRTVRNA
                     GYSLTSHD"
     gene            794..2224
                     /locus_tag="VNZ06_07405"
     CDS             794..2224
                     /locus_tag="VNZ06_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW20615.1"
                     /translation="MRFPRSSALTLAAGYIALGLAALALFAAPLWYAWRVTIQDGRYE
                     ILQSDALRLTDVFRREGAAGLVGYIDNRVGMQIAGERLLLLTDAAMKPMAGNMAAWPQ
                     NVPPTPGPYIVKLPMSGHLEDVALVHSTLAGGYHLLVGRDIARYVPLQKRFWYALASA
                     VGILSVVGTLGGILIRRALMTRIHGIRQTVSAIIQGDLSHRLQTQRSGDELNTLSQTI
                     NGMLDQIEQLVQGVRNVSNSIAHDLRTPLAELRSRLEELALTRPDSTHTFAQIDAAVA
                     DVDRVIGIFNALLRLAEIDSGLRRSGFVPVDLGEIASAAVDFYQPAAELRNARLVLES
                     SGSAPVRGDPVLLAQALGNLIDNSLKYAPEHSTITVAVGKVANQGVEIVVADRGPGIP
                     DAEKPKAIERFYRGDVSRGTPGVGLGLSLVDAVARLHGSELKLEDNQPGLRARMLLSQ
                     DNAKLVRPTRTANEEEEKYHHEPVYP"
     gene            2205..2717
                     /locus_tag="VNZ06_07410"
     CDS             2205..2717
                     /locus_tag="VNZ06_07410"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HWW20616.1"
                     /translation="MSLSIHDAAVPLTIRAMRALSGVLDKARAHAEAEKWDQSVLLSM
                     RLYPDMFPLSRQVQVVSDQCKGLIARLAAIDPPKYADTEATFAELKERLDKTIEFVKG
                     VDAKKFQGADERAIELKFPNNTLNFNNGWEYFLAFSVPNIYFHCSMAYGILRNCGVKI
                     GKPDFMGRIS"
     gene            2792..2989
                     /locus_tag="VNZ06_07415"
     CDS             2792..2989
                     /locus_tag="VNZ06_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20617.1"
                     /translation="MKLTVAVLALLSVCLSAAPVMALATESGASNSGYQATHHRHHHH
                     HRRHHKMDDTRSGGTQNPAIV"
     gene            complement(3005..3436)
                     /locus_tag="VNZ06_07420"
     CDS             complement(3005..3436)
                     /locus_tag="VNZ06_07420"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008008030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWW20618.1"
                     /translation="MKRSASAIWSGDLKTGHGALATHSGVLKETPYSFATRFEAAPGT
                     NPEELIAAAHAGCFTMALSASLGKAGFTPKRLATQATVSLELVNGNFTITAVALENEA
                     WVPGITAEKFEQIAADAKANCPVSRLLKADISLKAKLEAKS"
     gene            3526..4146
                     /locus_tag="VNZ06_07425"
     CDS             3526..4146
                     /locus_tag="VNZ06_07425"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWW20619.1"
                     /translation="MDEPQWLLWARELQAIAQTGLTFSKDNYDLDRYRRLRAIAVEML
                     AVGSNSSIEHIQGLFDQDLGYPTPKVDVRAAVFRDDRILMVREISDGRWTLPGGWADV
                     NQSPRECVEREVREESGFEAHAVKLAAVYDRRLSNPSAHPYSIYKLFFICELIGGAAS
                     PSIETSAVEFFARDQLPELSLGRTSPIQVERMYEHRTDPERPTDFD"
     gene            4157..4933
                     /locus_tag="VNZ06_07430"
     CDS             4157..4933
                     /locus_tag="VNZ06_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWW20620.1"
                     /translation="MKKNWLTVILVAFTSIPVWSAQEPLYLLPSDADMLRLLAPPPAV
                     DSQAQRDDLKAVLEAQREARAHNTTEHAIEDSQLSCGRVGDALGDTGVTSRVPQVFEF
                     LTEAGLEAAGLTSPPKTFWKRARPFAFSTDVEALGDMAKGKAPATQVPGFSSAAMSGA
                     DPAHMSYPSGHSTFGTVCAILLAEMVPEKRAELFARGLDYAHSRMVLGAHFPTDLEGG
                     RLTGTVAVDLLMHNPRFQHDFVNERASLRSALGLSTEPSN"
     gene            complement(4941..5747)
                     /locus_tag="VNZ06_07435"
     CDS             complement(4941..5747)
                     /locus_tag="VNZ06_07435"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HWW20621.1"
                     /translation="MNSGPRPTLHVRTIFISDVHLGSRDCRADLVLDFLRRVQTDQLV
                     LVGDIIDVWSLKRSFYWPQSHSDVLRTILGKTKHGTRVIFVPGNHDEQFRNVCGMAFG
                     NLEVQPEFVHETADGRRLLVMHGDEFDGAVKCSRWLASLGSGLYDLILRLNRTVNAWR
                     HRFGLGYWSLAGYLKSRVGNAMKYVHNFEQAAAHAARRRGLDGIVCGHIHRPEMVDID
                     GVLYCNDGDWVDSCSALVEHRSGELELWSWTDVNASVPELHRARGLVRAA"
     gene            6281..7408
                     /locus_tag="VNZ06_07440"
     CDS             6281..7408
                     /locus_tag="VNZ06_07440"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWW20622.1"
                     /translation="MGIDRKLPVVSLALLAVGGVAFADDAPPAPKYTGPSIADIIQGS
                     GLTATGYVSGTYSFQSYSASGEAAPKDTNSFILQQAAFTLADQPTAGFGALVNVIAGQ
                     NIYTPNYGVAGASNKATEFQLAQAYVQYIGGPVTVIAGKFVTLAGAEVLASVGNTNIT
                     RSLLYSFEPVTHTGVRLTYALNDQINLIVGANNGWIYSDEVSAGTGKTLEAGVAWTPS
                     KTFTWSLQGYTGEDTNFVGTTSHHSLIDTAATWNVNSALSLVGSVDWGQVDRHPFGLA
                     AGTPGSADWYGVAGYINYALSDTWRVSGRAEYFDDRKGYLTSVGPDVGNRLYEGTLDF
                     GYAPVKSVELRLEARYDQYNTSTTDVKVKVTQGWLEALYKF"
     gene            7450..8859
                     /gene="glnA"
                     /locus_tag="VNZ06_07445"
     CDS             7450..8859
                     /gene="glnA"
                     /locus_tag="VNZ06_07445"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012721762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HWW20623.1"
                     /translation="MSSSDVLKLVKDKEVKWADLRFTDTRGKEQHVSIPARYITNDFF
                     VEGKMFDGSSIAGWKGINESDMILLPDADTAVIDPFFEEATVNIRCDIIEPATMQGYE
                     RDPRSLAKRAEGYLKSTGIADSALFGPENEFFIFDSVRWGADLRSAFYEIDSVQGAWN
                     SDKQYDEGNKGHRPGVKGGYFPVPPVDAFQDIRSAMCRACEEMGLTVEVHHHEVATAG
                     QSEIGIGANTLVKKADEVQILKYCLHNVGANFGKTVTFMPKPLVGDNGSGMHVHQSLS
                     KEGKNIFAGDKYAGLSDTCLYYIGGIIKHARAINAITNSTTNSYKRLVPGFEAPVMLA
                     YSARNRSASIRVPWVSSPKARRIEVRFPDSTANPYFAFAAMMMAGLDGIQNKIHPGDP
                     ADKDLYDLEPEEARHIPTVCHSLDMALEYLDKDREFLKRGGVFTDDVIDAYIALKMQE
                     VTRLRMTTHPVEFDMYYSV"
     gene            8929..9498
                     /locus_tag="VNZ06_07450"
     CDS             8929..9498
                     /locus_tag="VNZ06_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HWW20624.1"
                     /translation="MRRLITCGLAVLTVLVLSGGGALATTLYRWVDAQGVVHYSDTPQ
                     PGAQKVQVQDAQTYKALPAPRIATPDSGGSPADDSQGRESAARAYQCSITAPMPDQSF
                     FSPESVSISVNVVPSLSRGDKLQVTLDGNALPMSRRGGTEVQAPDRGAHTINATVLSA
                     DGKTACTAEPVTFNVERPSLLSPQSPAHH"
     gene            9526..10629
                     /gene="glnL"
                     /locus_tag="VNZ06_07455"
     CDS             9526..10629
                     /gene="glnL"
                     /locus_tag="VNZ06_07455"
                     /inference="COORDINATES: protein motif:HMM:NF008293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HWW20625.1"
                     /translation="MFEPIAPSPLSHFDPAELLDVLSTGVVMLDVHLCVVYANVGAQD
                     LLAVGLNKARGRPIGELFSESQILDAILRRSLGRNETCAGHEILLIPMAASGPRDPVV
                     VDITVTPLEGQVTGTHLLLELADARQRQRITRESEMLSRLDGSRLMIRQLAHEIKNPL
                     GGLRGAAQLLDRELHDGALKEYTTIIINEADRLRSLVDSMLGPSQPQLLELVNIHELC
                     ERVFHLIRSEGAPGVVIDRDYDPSLPSGKFDRNQIIQALLNVARNALQAVDSNSGHVT
                     LRTRALSNINIGTARHRLVANLQVEDNGHGVPPELHRSVFYPLVTSRPSGTGLGLAVA
                     QDLVTRHRGIIEFESRPGHTVFSLLLPIEGTEE"
     gene            10626..12044
                     /gene="ntrC"
                     /locus_tag="VNZ06_07460"
     CDS             10626..12044
                     /gene="ntrC"
                     /locus_tag="VNZ06_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HWW20626.1"
                     /translation="MTAQPLRVWLVDDDTSIRWVLERALRNGGMLPKAFDAAEPALAA
                     LRNDTPDVLITDIRMAGQSGIDLLRKVHDARPELPVIVMTAYSDLGSAVSAYESGAFE
                     YLAKPFDIDQAVDLVKRAAAAGARSSESVSTVAPPIPELLGRAPAMQQVFRAIGRLSR
                     SSVNVLVTGESGTGKELAARALHEHSPRANKPFIALNTSAIPAELLESELFGHEKGAF
                     TGADSLRRGRFEQADGGSLFLDEIGDMSTSLQTRLLRVLAEGEFYRVGGQTPIRVDVR
                     VIAATHQNLEDRVKSGLFREDLFHRLNVIRIELPPLRARPEDVGDLLEHYMRIAAQEL
                     GVEPKTLTTDAREKLSVYPWPGNVRELVNLCRRFTVLAPGNEIRSEDLPSEVTGAASN
                     VPAEQDWTSALALWADRQSLSPPRPLLDDALPAFERTLIRVALRHTQGHRQEAAKLLG
                     WGRNTLTRKLKELSMDDIDDQD"
     gene            12088..13182
                     /locus_tag="VNZ06_07465"
     CDS             12088..13182
                     /locus_tag="VNZ06_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HWW20627.1"
                     /translation="MADWSQLARQTLLNNSATAWIFGALAFVLTFALFPLLRGLLRAQ
                     RRRHVNREPPVAIALLTDLVEHTSRFVLWIWALYVAEKILTLPKNLDRVFDVAIVVGI
                     WMQIGIWTAAAARFGLQRQQARTGDPRLTSTMDILLFVLRLIIWAVVALLALENLGIN
                     VGPLVAGLGVGGIAIALAVQTVLSDLFASLSIALDKPFVIGDSLRIDNLEGTVEQIGI
                     KSTRLRSVSGEQIILANADLLKSRVRNLGRASERRGLFMMSVAYDTPTAKLEQIPKLV
                     GDAVASYQGARFVYCLLKELGESGLQFEVCFFVENRPGRDVTYALDQINRQILAGFER
                     VGIGFAHPARTLWVKASSLSTAAPMVAPDA"
     gene            complement(13215..14567)
                     /locus_tag="VNZ06_07470"
     CDS             complement(13215..14567)
                     /locus_tag="VNZ06_07470"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWW20628.1"
                     /translation="MPRDQNPTGLKPDPILPESATHAAVAWCGAITMLGTCAVLNFWP
                     PFKNIAYLALIVMACTALGIFIPDLLWQRVQRRTLLPTPEAGNLDRTLTKCIGVAGSV
                     GFVALLYWLTPEYHNGESFYGHYYQALRVLLPAWAVAAPFYIYWVDRRLAQPHDSLWQ
                     LGRLLCGHWQDVDARVIWQHLLGWLVKGFFLPLMFTYFCDNLDALLHYDLNRLQSFKG
                     IFDWTYFTLYFIDVSLVSMTYLMSLKATDTHIRSSEPTALGWISALVCYEPFWTLISG
                     HYISYDNGKGWQDWFVSGTALWGAWACLILLLVVIYVWATIAFGARFSNLTHRGIITN
                     GPYRFTKHPAYLSKNLSWWMISMPFMFNGNWTDTLRHSLLLASLNLVYYLRAKTEERH
                     LSLDPVYVQYAQWIDEHGILRGLNNIPILGALARWRPVFRSYTPPIPIAENAPNATDA
                     "
     gene            complement(14609..15076)
                     /locus_tag="VNZ06_07475"
     CDS             complement(14609..15076)
                     /locus_tag="VNZ06_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017903159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWW20629.1"
                     /translation="MPLDRYDRRILEWLQKDGRLTNLELAEKIGLSPSPCLRRVRALE
                     DSGIIQGYRAVLDARKLGLTLTALVYIQMDRHTPERFAHFDAKVRSLPGVLECLLITG
                     QDADYQLKVVAQDMQAFEHLLLEQITRIEGVTGVHSSFVLRRVVDHTALPLGS"
     gene            complement(15371..15547)
                     /locus_tag="VNZ06_07480"
     CDS             complement(15371..15547)
                     /locus_tag="VNZ06_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20630.1"
                     /translation="MDRDAKAACGDIGAAQLDIGGGARRGARANRPLLIGLQADRQID
                     GRTLRSRLIGSNNA"
     gene            complement(15659..16885)
                     /locus_tag="VNZ06_07485"
     CDS             complement(15659..16885)
                     /locus_tag="VNZ06_07485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWW20631.1"
                     /translation="MKWLFGLTLVARLLFPFFDSPLTHLYSDPQRHWDNARLFLHPNI
                     LGSNDPYLYQAWLFAVRWISNDHAPGVALACGLLCAAMPYGWYRALRELLPRPWALAG
                     ALLIALLPETFSMYAYFMNETLLMTLLGFCFWLTLRSHRKGSPGAYALATLAWTCAAM
                     TRTVALPMACGSMAALWLLQSQRLLKLTLSAVIAAVVVVPAGLHAQTNLHFFAPFGNL
                     YFNSIYHDSGEHDIRVDYGPLGIYQFGAPSFYNPTYWPFSPWLTERRGTASITIDTVH
                     GTADWKREQTRIRRQRQFPAWRQRWEDFQYLLLGQEWPNNDAGTYIGEATLWARWMWP
                     PVLLFLLLAVARGWFRGTAWLLPVCALGTFLVLAMQTHGVTEARFRVPIDELFAACAI
                     CAIYFASGGTQSRPLR"
     gene            complement(16882..17205)
                     /locus_tag="VNZ06_07490"
     CDS             complement(16882..17205)
                     /locus_tag="VNZ06_07490"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HWW20632.1"
                     /translation="MSIAEELEKLQMLRDRGTISEQEFVRAKQQALDEATARTGSNSA
                     LLYQLARSRTDRMLGGVCGGLGKQTDLPAWAWRIIFILTFVYFGAGLVVYLLLWIFLP
                     EERAE"
     gene            complement(17224..18204)
                     /locus_tag="VNZ06_07495"
     CDS             complement(17224..18204)
                     /locus_tag="VNZ06_07495"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HWW20633.1"
                     /translation="MDVAAKPSGPESSLAVQFCGLEFASPIVLLSGCVGFGEEYTRVE
                     GFSNRDVGAVVLKGTTGAPRLGNSAHRVYETPGGMLNAIGLQNPGVDHVVREILPRLD
                     FSETRFIANVCGSTIEEYVEVTRRFDDSPIDAIEINISCPNIKEGGVQFGNVPEMSAR
                     VVEACRRVTDKPLITKLSPNQTDIRTNARLCIDAGTDAFAVINTVTGMAIDIDTSRAF
                     LGNTQGGLSGPAIKPIALLKVREVYEVAGPGRIPIIGQGGITTASDAIEFIIAGASTV
                     GVGTALFYDPLVCQSINAGIARYLAAHGLGGVAQLCGTLHGAVRDLKAFC"
     gene            18241..18726
                     /locus_tag="VNZ06_07500"
     CDS             18241..18726
                     /locus_tag="VNZ06_07500"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003171120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HWW20634.1"
                     /translation="MFHVILYEPEIPPNTGNIIRLCANTGASLHLVGPLGFDLERRSV
                     RRAGLDYDELASVSRHRNLAQCLKRLPDARLFAVETGSHTRYADRSYRPGDAFLFGPE
                     TRGLPTEVLASLSAEFRITIPMRPGNRSLNLGNAVAVVLYEAWRQNGYRSPTTSVKLC
                     R"
     gene            complement(18687..19517)
                     /locus_tag="VNZ06_07505"
     CDS             complement(18687..19517)
                     /locus_tag="VNZ06_07505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWW20635.1"
                     /translation="MTAKGLLGDSAGRDYSRKLSLFNACAQPELRQAIASLQLRPGMH
                     AVDVGCGTGETLGWLQAALQPGGRVVGVDLAAAHLQAARRHSPADILIAQADALRLPL
                     RAAHFDLVWCVNTLNHLREPVEAIRQWGTLLRNSGRIALGQSSLLPEMFFAWDARLER
                     IVNDAVRQYYRERYGVSERELTAVRALLGVLRQAKLRNVWSRTFMIERISPLHAVDEI
                     YLTEAIFRDTWGERLRPYLEACDYAELCRLCDPADPAFALRRPDFHYLQSFTLVVGER
                     "
     gene            complement(19529..>20006)
                     /locus_tag="VNZ06_07510"
     CDS             complement(19529..>20006)
                     /locus_tag="VNZ06_07510"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HWW20636.1"
                     /translation="YTSTDITGVEVGGAVKNVLAIGAGLSDGLGFGANTRIALITRGL
                     TEMTRLGVALGARRETFMGLAGLGDLVLTCTDDHSRNRRFGLALARGASVDAALEEIG
                     QVVEGYQAARALRVVATREGVVMPIAEGLYGVLYEQLAVEQVVRGLMLRPIKPEFD"
BASE COUNT         3880 a         6145 c         6268 g         3713 t
ORIGIN      
        1 tttatctgta tctggatcag ttcaccggcc agcgccggca cgcgcgtgcg aagcaggcga
       61 ttgcagacgg cgggaaggcc atcccgcaag gggccgcctc atgaaagtac tgctggtgga
      121 agacaacgag cgcgtgacgc agttcgtcgt caaaggactg caggaggcgg ggcacaccgc
      181 agaccatgcc gccaacgggc gagatggcat gtttctggcc gccagcgagc cgtatgacgt
      241 catcgtgatg gaccgtatgc tgcccggcga tatcgacgga ctggccatca tcgaagcgct
      301 gcgcaaatcc ggtaaacgca cgccgatcct gattttgagc gcactggccg aggtggatga
      361 gcgcatcaag ggtctgcgcg ccggcggcga tgactatctc accaagccct ttgcgttcgg
      421 cgaactgctg gcgcggctgg atgcattgac ccggcgggcg cagggtgaga acgcccagac
      481 gcgtctggaa gtcgcggatc tgcgagtgga tctgctgacg cgcaaagtga cacgcggcaa
      541 tcgggggatt acgctgcagc cgcgcgagtt caagctgctc gagtacctga tgcggcatgc
      601 cgaccaggtg gtcacacgca ccatgctgct ggagaatgtc tgggactatc atttcgatcc
      661 gcagaccaat gtggtcgatg tgcacatcag caagctgcgc cagaagatcg atgccgactt
      721 cgaacgaccg ctgctgcgca ccgtacgcaa tgcggggtat tcgctgacct cgcacgactg
      781 agcaggaggg ctgatgcgct tcccccgttc ctctgcactg acgctggccg ccggctacat
      841 cgctctggga ttggccgcgc tggcgctgtt cgccgcgccg ctatggtacg catggcgtgt
      901 cacgatccag gacggacgct acgagattct gcagtccgat gcgctgcgtc tcaccgatgt
      961 gttccgccgc gagggcgctg cgggcctggt cggctacatc gacaaccgcg ttggcatgca
     1021 gatcgcgggt gagcgcctgc tgctgctgac cgacgcggcg atgaagccaa tggcaggcaa
     1081 catggcggcg tggccgcaaa acgtgccacc gacgcccggc ccctacatcg tcaagctgcc
     1141 gatgagcggt catctggaag atgtcgcgct ggtgcactcg actcttgccg gcggctacca
     1201 cttgctggtc ggacgcgata tcgcacgcta tgtgccgttg cagaagcgtt tctggtacgc
     1261 gctggcgagc gcggtgggca tcctgtcggt ggtcggaaca ttgggcggca tcctgatccg
     1321 gcgagcattg atgacgcgca tccacggcat ccggcagacc gtgtcggcga ttattcaagg
     1381 tgacctgagt catcgactgc agacacagcg cagcggcgac gaactcaata ccctgtcgca
     1441 gacgatcaac ggcatgctcg atcagatcga gcagctcgtt cagggcgtac gcaacgtatc
     1501 aaactcgatc gcgcacgatc tgcgcacgcc gctggccgag ctgcgctctc gcctggagga
     1561 actggcgctc accaggccgg atagcactca cacctttgcg cagatcgatg ccgcggtcgc
     1621 ggatgtcgat cgagtcatcg gcatcttcaa tgcgttgctg cgattggccg agatcgacag
     1681 tgggctgcgc cgctccggtt tcgtgccggt ggacctgggc gagattgcat ccgccgcggt
     1741 tgacttctac cagcccgccg ccgagttgcg caacgcccgt ctggtgctgg aaagctccgg
     1801 ctctgcgccg gtgcgcggcg atccggtgct gctcgcgcag gcgctcggca atctcatcga
     1861 caactcgctc aaatacgcgc ccgagcacag cacgatcacg gtcgctgtgg gcaaggtggc
     1921 gaatcagggt gtcgagatcg tcgtggcgga tcgcggcccg ggcattcccg acgcggaaaa
     1981 accgaaggcg atcgagcgct tctaccgggg cgatgtcagt cgtggcacgc cgggcgtggg
     2041 cctgggactg agtctggtcg atgccgtggc gcggctgcac gggagcgaac tgaagctgga
     2101 agacaatcag ccggggttgc gggcgcgcat gctgctgagt caggataacg ccaagctcgt
     2161 gcggccgacg cgcacggcga acgaagaaga ggagaaatat caccatgagc ctgtctatcc
     2221 atgatgccgc cgttccgctg accatccgcg caatgcgcgc cctgtccggg gtgctcgaca
     2281 aagctcgtgc gcacgcggag gccgagaagt gggatcagtc cgtattgctg tccatgcggc
     2341 tctatccgga tatgtttccg ttgagccgtc aggttcaggt cgtatccgac cagtgcaaag
     2401 gtctgatcgc gcgtttggca gcgatcgatc cgccgaagta cgccgatacc gaggccacgt
     2461 ttgcggagct caaggagcgt ctggacaaga ccattgagtt cgtcaagggc gtggatgcga
     2521 agaagttcca gggtgccgac gagcgtgcga tcgagctgaa atttcccaac aacacgctga
     2581 acttcaacaa tggctgggaa tactttctcg cgttttcggt gccgaatatc tatttccact
     2641 gctcgatggc gtacggcatc ctgcgcaact gcggcgtcaa gatcggcaag ccggatttca
     2701 tgggaaggat ttcatgacga caagctcgaa atcgcgttaa gattgcggcg ccccaacttg
     2761 tgggcgcttg taaatttccc aggatcgcga aatgaagctc acggttgccg ttctggcctt
     2821 gctcagcgtc tgcctgtccg ctgccccggt catggctttg gctaccgaga gcggcgcttc
     2881 gaacagcggc taccaggcga cccaccatcg ccatcaccac caccatcgcc gccatcacaa
     2941 gatggacgac actcgcagcg gcgggacgca gaaccccgcg atcgtctgat cgatcctttt
     3001 acgcttaaga cttggcctcg agctttgcct taagcgagat atcagccttt aacagacgcg
     3061 acaccgggca gttggccttg gcgtcggcgg cgatttgttc gaatttctcc gccgtgatac
     3121 caggcaccca ggcctcgttt tccagcgcta cggcggtgat ggtgaagttg ccgttgacca
     3181 gctcaagcga aacggtggcc tgagttgcca gtcgtttcgg cgtgaacccg gccttaccca
     3241 ggctcgcact caatgccatc gtgaagcagc cggcgtgcgc tgcggcaatg agttcctcgg
     3301 gattcgtacc cggcgctgcc tcaaagcggg tcgcgaagct gtaaggggtt tccttgagca
     3361 cgccgctgtg tgtcgccagc gccccgtgcc cggtcttcag atcgccggac cagatggccg
     3421 atgcggatcg cttcatggaa atactccttg agtggctggt ccgacgcgtg tgtcgtgcgc
     3481 cgactgtact gctagagttg cgcaaacgcc aaggatgatc cgccgatgga tgaaccgcag
     3541 tggctgctct gggcgcgcga gctgcaggcg atcgcgcaga ctggtctgac cttctcaaag
     3601 gacaattacg acctcgaccg ctaccgccgc ctgcgtgcga ttgccgtcga gatgctggca
     3661 gtgggatcca acagttcgat cgagcacata cagggattgt tcgatcagga tctgggctac
     3721 ccgacgccga aagtggatgt gcgggccgcg gtgtttcgcg acgaccgaat cttgatggtg
     3781 cgcgaaatca gtgacgggcg ttggaccctg ccgggcggat gggccgacgt caatcagtcg
     3841 ccccgggagt gcgtcgagcg cgaggtcagg gaggagtccg gcttcgaggc acatgcggtg
     3901 aaactggccg cggtatacga ccggcgcctg agcaatccgt ccgcccatcc gtactcgatc
     3961 tacaaactat tcttcatctg cgagctgata ggcggtgcgg ccagcccgag catcgagacc
     4021 agcgcagtgg agttctttgc gcgcgatcag ctacccgagc tgtcactcgg ccgcaccagc
     4081 ccgatacagg tcgagcgcat gtacgagcac cggactgatc cggagcggcc cacggatttc
     4141 gactaggagc caccccatga agaagaattg gctgactgtg atcctcgtag ccttcaccag
     4201 catccctgtg tggtcggcgc aggagccgct gtatctgctg ccgtcggatg ccgacatgct
     4261 gcgcctgctg gctccgcctc cggcggttga ctcgcaggcg cagcgtgacg acctgaaagc
     4321 ggtactcgag gcgcagcgtg aggcgcgtgc acacaatacc actgagcacg cgatcgagga
     4381 ctcgcagctg agctgcgggc gcgtagggga tgccctcggt gacaccggcg tgaccagtcg
     4441 cgtgccacag gtgttcgaat ttctaacgga agccggcctt gaggccgccg ggctgaccag
     4501 tccaccgaag accttctgga agcgggcccg accgttcgcc ttcagcacgg atgttgaagc
     4561 actgggcgac atggcgaagg gtaaggctcc ggccacgcaa gtaccaggat tcagcagcgc
     4621 ggccatgagt ggggccgatc ccgcgcacat gtcctatccg agcgggcatt ccacatttgg
     4681 taccgtatgc gccattctgc tcgccgagat ggtccctgag aaacgggccg agctgtttgc
     4741 tcgcggccta gactatgcgc attcaaggat ggtgcttggc gctcacttcc caacggatct
     4801 ggagggcgga cgcctcacag gtacggtcgc ggtggacctg ctgatgcaca acccaagatt
     4861 ccagcacgat tttgtcaacg aacgcgcgag cctgcgctcc gcgttagggc tatcgactga
     4921 gccttcgaac tagaaggggt tcaagcggcg cgtaccaggc cgcgcgcgcg atgaagttcc
     4981 ggtaccgacg cgttgacgtc tgtccagctc cagagctcca gttcgccgct gcggtgctcg
     5041 accagtgccg agcaactgtc gacccagtcg ccgtcgttgc agtagagaac gccgtcgata
     5101 tccaccattt cgggtcggtg tatatgcccg cagacgatgc cgtccaagcc gcggcggcgt
     5161 gcggcgtgag cggccgcctg ctcgaaattg tgaacgtact tcatcgcatt gccgacacgg
     5221 ctcttgaggt aaccggccag tgaccagtac ccgagaccga agcggtgccg ccacgcgttg
     5281 acggtgcggt tcagcctcag gatgaggtcg tacaacccgg agccgagcga ggcgagccag
     5341 cggctgcatt tcacagctcc atcgaactca tcgccgtgca tgaccaacaa gcggcgtcca
     5401 tctgcggttt cgtgcacgaa ttcaggctgc acttccagat tgccgaaggc catgccgcac
     5461 acattccgga actgctcgtc gtgattgccc gggacgaaaa tgacgcgggt gccgtgcttg
     5521 gtcttgccga gaatcgtgcg cagtacgtcg ctgtgtgact gtggccagta gaaactgcgc
     5581 ttcagactcc acacatcgat gatgtcgccc accagaacga gctgatcggt ctgtacccga
     5641 cgcagaaaat ccaggaccag atctgcccgg cagtcgcgcg agcccagatg aacgtcagaa
     5701 atgaaaatcg tgcgtacgtg caaagtcggt cgagggccag agttcattca cgcctctgaa
     5761 agggcctaca gccggcccat agtcttgtgc tgcgggatga caatcccatg gcaagccagt
     5821 gacatagcga tgaatattga tgtcgcgtga gacaaattcc gcggaagcgg ctgagccata
     5881 gcaagtgctt gagtctctgg gtgcaacgca aggtcacttt cgaatggcgt gtgagttctc
     5941 gaaaggtcca gtcagaccag gcaggcgctg caccactgcg atgcaaacgc ggcgtccgca
     6001 gtcaccaaat gagtgcgaca ctccccatcg cctggcgctc gtgcggcgtt agaccttcaa
     6061 tggctggcac agaacgggaa agttatgagc ttatccgcgt taagaaattg ttaagaattc
     6121 cggcgcatct ctcgttcctt tgctcgctcc gcgggcccgt ctccgcgatg tagcacccat
     6181 gtttcggccg gttggcatgt cgcatgcaaa tcgctcgcgg gcgcgagacg gggacagctg
     6241 ctgtggctga cgttctcgac aacactaaag ggagtttcaa atggggatcg atcgaaagtt
     6301 accagtcgtc tcactggctc tgctggccgt tggcggcgtt gccttcgcgg atgatgcgcc
     6361 gccggcgccg aaatatacgg ggccgagcat tgccgacatt atccaagggt cgggactgac
     6421 ggctacgggc tatgtcagtg gcacgtattc tttccagagc tactcggctt ccggcgaggc
     6481 ggcgccgaag gacaccaaca gcttcattct gcagcaagcg gcgttcacgc tcgccgacca
     6541 acccacggcc ggcttcggcg cactggtgaa tgtcatcgct ggccagaaca tctatacccc
     6601 gaactacggt gtggcaggag cttccaacaa agcgaccgag ttccagctcg cgcaggccta
     6661 tgtccaatac atcggcggcc cggtgaccgt gattgccgga aagttcgtga cactggcggg
     6721 cgctgaagtc ctcgcctccg tcggcaacac caacatcact cgctcgctgc tgtattcatt
     6781 cgaaccggtc acgcataccg gtgtgcgcct gacgtacgca ctgaacgatc agatcaacct
     6841 gatcgtcggt gccaacaatg gctggatcta cagcgatgag gtctcggccg gcaccggcaa
     6901 gaccctcgaa gcgggcgtgg cgtggactcc ctcgaagacg tttacctggt cattgcaggg
     6961 ctacaccggt gaggacacca actttgtcgg taccaccagt catcactcgc tgatcgacac
     7021 ggcggccacc tggaatgtga actcggcgct gagcctcgtc ggcagcgttg actggggaca
     7081 ggtcgatcgt catccgttcg gcctggcggc tggcaccccg ggtagtgccg attggtacgg
     7141 cgttgccggc tacatcaact atgctctgag cgatacctgg cgggtatccg ggcgcgctga
     7201 atacttcgac gaccgcaagg gctatttgac gagtgtcggt ccggacgtcg gcaaccggtt
     7261 gtacgaaggc acgctggatt ttggctatgc tccggtcaag agcgtcgaac tgcgactgga
     7321 agctcgctac gaccagtaca atacgagtac caccgacgtg aaggtcaagg tcacacaggg
     7381 ttggcttgaa gctctctata aattctgagc gatgcgtacg ctccatcatc aggccggtca
     7441 ggagacccaa tgagttcgag tgatgtgctg aaactggtca aagacaaaga ggtgaagtgg
     7501 gccgacctgc gcttcaccga cacgcgtggg aaggagcaac acgtcagcat ccccgcccgc
     7561 tacatcacga atgatttctt cgtcgaaggc aagatgttcg acggctcgtc gatcgccggt
     7621 tggaagggca tcaatgagtc cgacatgatc ctgctgccgg acgccgatac ggcggtgatt
     7681 gaccccttct tcgaggaggc cacggtcaat attcgctgcg acatcatcga gccggcgacc
     7741 atgcagggtt atgagcgcga tccgcgttcc ctcgcgaagc gcgcggaagg ctacctgaaa
     7801 tcaaccggca ttgccgatag cgcgctgttc gggccggaga acgaattttt catttttgac
     7861 agcgtccgct ggggcgcgga tcttcgcagc gcgttctacg agatcgactc tgtgcagggc
     7921 gcctggaact cggacaagca atatgacgag ggcaacaagg gccatcgtcc gggcgtcaag
     7981 ggcggctact ttccggtacc tcccgtcgac gcctttcagg acatccgctc ggcgatgtgt
     8041 cgcgcgtgcg aagagatggg tctgacggtg gaagtgcatc accacgaagt cgcaaccgcc
     8101 ggccagagcg aaatcggtat cggtgccaac acgctggtga agaaagccga tgaggtgcag
     8161 atcctcaagt actgcctgca caacgtcggc gcaaactttg gcaagaccgt gaccttcatg
     8221 cccaagcccc tggtgggcga taacggcagt ggcatgcacg tgcatcagtc gctgtcgaaa
     8281 gagggcaaga acatttttgc cggcgacaag tacgcgggtc tgtccgatac ctgcctgtac
     8341 tacatcggcg gcatcatcaa gcacgcgcgc gcgatcaacg cgatcaccaa ttcgaccacc
     8401 aacagctaca agcgcctggt gcccgggttc gaggcgccgg tgatgctggc ctattcagcc
     8461 cgcaatcgct ccgcctcgat ccgcgtgccg tgggtgtcga gcccgaaagc gcgtcgcatc
     8521 gaagtgcgtt tcccggattc gaccgccaat ccgtacttcg cgttcgctgc catgatgatg
     8581 gcgggcctcg acggcattca gaacaagatt catccgggcg atccggccga caaggatctg
     8641 tatgacctgg agccggaaga ggcgagacac atcccgaccg tgtgtcactc gctcgatatg
     8701 gcgctcgagt atctggacaa ggaccgtgaa ttcctcaagc gcggcggcgt attcaccgat
     8761 gacgtcatcg atgcctatat cgccttgaaa atgcaggaag tgacgcgtct tcggatgacc
     8821 actcacccgg tcgaatttga catgtattac agcgtctgag gtaaacgcca agcgtggccc
     8881 ccacccgatt ttgtatgccg ggccctccgg gggctatgct tggctttcat gcggcgccta
     8941 atcacatgcg gcctggcggt gctgaccgtg ctcgttttga gcgggggcgg cgcgctggcc
     9001 accacgctct atcgctgggt ggacgcgcag ggggtggtgc actattccga tacgccgcaa
     9061 ccgggcgctc agaaagtgca ggtgcaggac gcgcagacgt acaaggcgct gccggcgccc
     9121 aggatcgcaa ctccggactc gggtggctcg cctgccgacg attcccaggg tcgggaatcc
     9181 gcagcgcgcg cataccagtg cagcatcacg gcaccgatgc cggatcagtc ctttttcagt
     9241 ccggagtcgg tatcgatttc ggtcaacgtc gtaccgtcgc tcagtcgcgg ggacaaactg
     9301 caggtcacgc tggacggcaa tgcgctgccg atgtcccgcc gcggcgggac cgaagttcag
     9361 gcgccggacc gcggtgctca taccatcaac gcaacggtac tatccgccga cggcaagaca
     9421 gcgtgcaccg cagagccggt caccttcaat gtcgagcggc cgtcgctgct gtcgccgcag
     9481 tcacccgctc accactagcg cgcgctggcg cgcgggtgcg cgctggtgtt tgagccgatc
     9541 gcaccctcac cgctgtcgca tttcgatccg gccgaactgc tcgatgtcct gtcgaccggt
     9601 gtggtcatgc tggatgtcca tctgtgcgtg gtatacgcca acgttggggc acaggacctg
     9661 ctcgcggtcg ggctcaacaa agcgcgtgga cgcccgatcg gagagctgtt cagcgaatcg
     9721 cagatcctgg atgccatcct gcgacgctcg ctcgggcgca atgagacctg cgcgggacac
     9781 gaaattctcc tgataccgat ggcagcttcc gggcctcgcg atccggtggt ggtcgatatc
     9841 accgttacac cattggaagg gcaggtcacc ggaacgcacc tgctgctcga gctggcagac
     9901 gctcggcagc gtcagcgtat tacgcgcgag agcgaaatgc tgtcgcgact ggacggcagt
     9961 cgtttgatga ttcggcagct tgctcacgag atcaagaacc cgctcggcgg gttgcgcgga
    10021 gccgcccagc ttctggatcg tgagctgcat gatggcgcac tgaaggaata caccaccatc
    10081 atcatcaatg aagccgaccg actgcgctcg ctggtggaca gcatgctcgg cccttcgcag
    10141 cctcaactgc tggagctggt gaatattcat gagctgtgtg agcgcgtatt ccatttgata
    10201 cgtagcgaag gcgcacccgg ggttgtcatc gatcgcgact acgatccgag cctgcccagc
    10261 ggaaagttcg accgtaacca gattatccag gcgttattga acgtggcgcg caatgcattg
    10321 caggcggtcg attcgaacag cggtcacgtg acgctgcgca cccgcgcgct gtcgaacatc
    10381 aatattggca ccgcgcggca ccggctggtg gcgaatctgc aggttgagga caacgggcat
    10441 ggcgtgcctc ccgaactgca tcgcagtgtg ttctacccgc tggtcacgag ccgtccgtcc
    10501 ggcacgggac tcggtcttgc cgtcgcgcag gatttggtca cgcgccaccg cggcatcatc
    10561 gaattcgaga gtcgtccggg tcataccgtt ttctcactgt tactgccgat tgagggcaca
    10621 gaagaatgac tgcacaaccc ctacgcgtct ggctggtgga tgacgatacg tccatccgtt
    10681 gggtactcga gcgagcgctg aggaacggtg gaatgctgcc caaggccttc gatgcggcag
    10741 agccggcact ggccgccttg cgcaacgata cccccgatgt cctgatcacc gacatacgca
    10801 tggcggggca gtcgggtatc gatctgctgc gcaaggtaca cgacgcgcgc ccggagctcc
    10861 cggtcatcgt catgaccgca tactcggatc tcggcagtgc cgtctcggca tatgagagcg
    10921 gcgcattcga gtatctggca aagccgttcg atatcgatca ggcggtcgat ctggtcaagc
    10981 gtgccgccgc cgccggcgcg cgcagttcgg aaagtgtctc gacggtggcg ccgccgattc
    11041 cggaactgct ggggcgggca ccggcgatgc agcaggtctt ccgggcgatc ggccggctgt
    11101 cgcgctcgag cgtgaacgtc ctggtcaccg gtgagtcggg cactggcaag gaactcgcgg
    11161 cgcgcgccct gcacgagcac agtccgcggg ccaataagcc gttcatcgct ctcaatacgt
    11221 ccgccattcc cgcagaactg ctggaatcgg aactgttcgg ccacgagaaa ggtgcgttca
    11281 ccggcgctga cagtctgcgt cgcggccgct tcgagcaggc cgatggcgga tcactgttcc
    11341 tcgatgagat cggagacatg tccacctcgc tgcagacgcg gctgttgcgc gtgctggccg
    11401 aaggcgagtt ctaccgggtc ggcggtcaga ctccgattcg cgtggatgtg cgcgtcatcg
    11461 ctgcaaccca tcagaatctg gaggatcggg tcaaatcggg cttgttccgc gaggacctgt
    11521 ttcatcgtct caacgtgatc cgcattgagc tgccgccgct gcgggcgcgt ccggaagacg
    11581 tcggcgatct gcttgagcac tatatgcgta tcgcggccca ggaactgggt gttgaaccca
    11641 aaaccctgac tacggatgcg cgcgagaaac tcagtgtcta tccatggcca ggcaatgtcc
    11701 gcgaactggt caacttgtgc cgacgcttca ccgtgctcgc gcctggcaat gaaatccggt
    11761 cggaggatct gccttcggag gtgaccggcg cagcctcgaa tgtgccggcc gagcaggact
    11821 ggaccagcgc gctggcgctg tgggcggacc gacagtcatt gtcaccgccg cggccgttgt
    11881 tggacgacgc actgccggca ttcgagcgga cattgatccg cgtggcgctg cgtcacaccc
    11941 aggggcatcg gcaggaagcc gccaagctgc tgggctgggg ccgaaacaca ctgacgcgca
    12001 agctcaagga attgagcatg gatgacatcg acgatcagga ctgaaccgca gtgagatctg
    12061 ccgggcgcat tgttatattg cgcgccgatg gctgactggt cacaactcgc gcgtcagacg
    12121 ctgctgaaca acagcgccac cgcttggatc tttggcgctc tggcgttcgt cctgacgttc
    12181 gcactgttcc cattgctgcg cggcctgctg cgtgcgcagc ggcgccgcca cgtcaaccgc
    12241 gagccaccgg tggcgatcgc gctgctcacg gacctggtgg agcacaccag ccgttttgtc
    12301 ctgtggatct gggcactgta cgtggccgag aagatcctga cgttgccgaa gaacctcgat
    12361 cgcgtgttcg acgttgcgat cgtggtcggt atctggatgc agatcggcat ctggacggcg
    12421 gcggcggcgc gcttcggctt gcagcgacag caggcgcgca ccggcgatcc gcggctgacc
    12481 agcacgatgg acattctgtt gttcgtgctg cggctcatca tctgggccgt cgtcgcgctg
    12541 ctggcactgg agaaccttgg catcaacgtc gggccgctgg tggcgggcct tggtgtcggc
    12601 ggtatcgcga ttgcgttggc ggtgcagacg gtcctgagcg atttgttcgc atcgctgtcg
    12661 atcgcgctgg ataaaccgtt cgtcatcggt gactcgctgc ggatcgacaa cctcgaaggc
    12721 accgtcgagc agatcggcat caagagcacg cggctgcgga gcgtcagcgg cgagcagatc
    12781 attctggcga acgcggatct gctcaagagc cgggtgcgca atcttggacg cgccagcgag
    12841 cggcgcgggt tattcatgat gtcggtggca tacgataccc cgaccgcgaa actcgagcag
    12901 ataccgaagc tggtcggtga cgcggtcgcc agctatcagg gcgcgcgctt cgtttactgc
    12961 ctgttgaagg agctgggtga atccggactg cagttcgaag tgtgtttctt cgtcgagaac
    13021 cgccccggcc gcgacgtcac ctacgcgctc gatcagatca accggcagat tctcgctggg
    13081 ttcgagcgcg ttggcatcgg cttcgcccat ccggcacgaa cgctctgggt gaaagcctct
    13141 tccttgtcca ccgctgcgcc aatggtcgcg cccgatgcgt agcgatcaag gcacgtttgg
    13201 atgcacggca ccggttatgc gtcggttgcg ttcggcgcgt tctcggcaat gggtatcggc
    13261 ggcgtgtagc tcctgaacac gggccgccag cgcgccagcg cgcccaggat cggaatgtta
    13321 ttcaggccgc gcaagatgcc gtgctcatcg atccattgcg catactgcac atagaccgga
    13381 tccaggctca ggtgtcgctc ttcggtcttg gcgcgcaggt agtacaccag gttgaggctg
    13441 gccagcagca ggctatggcg cagcgtgtcg gtccaattgc cgttgaacat gaacggcatc
    13501 gagatcatcc accacgacag gttcttcgac agatacgccg ggtgcttggt gaagcgataa
    13561 ggtccgttgg tgatgatgcc tcgatgggtc agattggaga atcgcgcacc gaacgcaatc
    13621 gtcgcccaca cgtagatgac taccagcaag aggatcaggc aggcccatgc tccccagagc
    13681 gcggtgccgc tgacaaacca atcctgccat cctttgccgt tgtcatagct gatgtagtgg
    13741 ccgctgatca atgtccagaa cggctcgtaa cagaccagcg cactgatcca gcccagggca
    13801 gtgggctcgg atgagcgaat gtgggtatcg gttgccttca gcgacatcag ataggtcatc
    13861 gataccaggc tcacgtcgat gaagtacagc gtgaagtacg tccaatcaaa gatgcctttg
    13921 aagctctgca gtcgattgag gtcgtagtgc agcagcgcat ccaggttgtc gcagaaatag
    13981 gtaaacatca gcggcaggaa gaagcccttg accagccaac ccagcaggtg ttgccagatc
    14041 acccgggcat cgacatcctg ccagtgtccg cacaataacc gccccagctg ccacaggctg
    14101 tcgtgcggtt gcgccaggcg gcggtcgacc cagtagatgt agaacggcgc cgccaccgcc
    14161 catgccggta acagtacgcg tagcgcctgg tagtagtggc cgtagaatga ctcgccgttg
    14221 tggtactccg gcgtgagcca gtaaagcaag gccacgaagc ccacacttcc tgcgaccccg
    14281 atgcacttgg tcagcgtccg gtccaaattt ccggcctcag gcgtcggcaa tagcgtgcgt
    14341 cgctgcaccc gctgccacaa cagatcgggg atgaagatgc cgagcgccgt gcaggccatg
    14401 acgatcagtg ccagataggc gatgttcttg aacggcggcc agaagttgag caccgcgcag
    14461 gtacccagca tcgtgattgc gccacaccag gccaccgccg cgtgcgttgc cgattcgggc
    14521 aatatcgggt ctggcttaag gccggttgga ttctggtcgc gcggcatcga cgctccgttg
    14581 agtcagtaac tgcattgtgc cacccggatc agctgccgag cggcagtgcg gtgtgatcaa
    14641 ccactcttct cagcacgaaa ctcgagtgca ctccggtcac gccctcgatg cgagtgatct
    14701 gttcgagcag caggtgttcg aacgcctgca tgtcctgcgc gaccaccttg agctgatagt
    14761 cagcgtcctg cccggtgatc agcaggcact ccagcacccc gggcagcgaa cggaccttcg
    14821 cgtcgaaatg cgcgaagcgc tccggagtgt ggcgatccat ctgaatgtag accagcgccg
    14881 tgagcgtcag tccgagcttc cgcgcatcca gcaccgcacg gtagccctgg atgattccgc
    14941 tgtcttcgag cgcgcgcaca cgacgcaggc agggcgaagg cgagagtccg atcttctcgg
    15001 cgagctccag gttcgtgagt ctgccgtctt tctgcagcca ttccaggatg cgacgatcgt
    15061 aacgatccaa cggcatttat ttctcgctgc tgcggcggtg acagcaataa aatgccatag
    15121 atatcgcaat atcagcaata aatatctgca ttcgaggggt ggcctgccct catttcgcaa
    15181 tcgcgttgcg cgccgcgccg gctacgctgc aaagctgctg tgatgacaac tgcccggggt
    15241 ctgcgatgct gcgtgagccg caccgaaaat accgcccgtt tgcacaggta gcgctgaccc
    15301 agcggcaatg gccgggtagg agcatacgtc aggctccgat ctggctcagt acggatctgc
    15361 gcgatggcaa tcaggcgttg ttcgaaccga tgagccgtga acgcaaggtg cggccatcga
    15421 tctgccggtc agcctgcaat cctatgagga gcgggcgatt ggctcgggcg ccgcggcgcg
    15481 cgccgccgcc tatatcgagt tgagctgcgc cgatgtcacc gcaggccgct ttggcgtcgc
    15541 gatccacgcc aacatcgtca ccgcgtccct actggcgctg ttcagcggac tgaatcggct
    15601 gctgcgagcc atggacgagc gcacgcgaca ggcatcattg ctgcagctac ggcgtcagtc
    15661 atcgcagcgg cctcgattgc gtaccgcctg aagcaaagta gatggcgcaa atcgcgcagg
    15721 ccgcgaacag ttcatcgatc ggcacgcgaa agcgcgcttc ggtcacaccg tgggtctgca
    15781 tcgccagcac caggaaagtg cccagagcac agaccggcag cagccatgcg gtgccccgga
    15841 accagccccg ggcgaccgcc aacagcaaga acagcagcac cggcggccac atccagcgcg
    15901 cccacagcgt tgcctcgcca atgtacgtgc ccgcgtcgtt gttcggccac tcctgaccta
    15961 acagcagata ttgaaagtcc tcccagcgct ggcgccacgc ggggaattga cgctgccggc
    16021 ggatacgtgt ttgctcgcgc ttccagtcgg ccgtgccgtg aacggtgtca atcgtgatcg
    16081 acgcggtccc ccgtcgttcg gtcagccacg gcgaaaacgg ccagtaggtc gggttgtaga
    16141 agctgggggc tccgaactgg tagatgccca gcggtccata gtcgacgcgg atatcgtgct
    16201 cgccgctatc gtgataaatc gaattgaaat agagatttcc gaagggcgcg aagaaatgca
    16261 gattggtctg cgcatgcagg cctgccggta ccaccacgac ggcagcaatc accgccgaga
    16321 gcgtcaattt cagcagccgc tgcgactgca ggagccacag cgccgccatc gatccgcacg
    16381 ccatcggcag cgcaacggtg cgcgtcatcg ccgcgcaggt ccaggctagc gtcgccagcg
    16441 cgtaggcccc cggcgagccc ttgcggtgcg agcgcagcgt cagccaaaag caaaatccaa
    16501 gcagcgtcat cagcagtgtc tcattcatga agtaggcata catgctgaag gtttcgggca
    16561 gcagcgcgat gagcagcgcc cctgccagcg cccatggccg cggcagcagc tcgcgcaagg
    16621 cccgatacca gccgtacggc atcgccgcgc acagcaatcc gcacgccagc gcaacgccag
    16681 gcgcatggtc gttgctgatc catcgcaccg cgaacagcca ggcctgatag agatagggat
    16741 cgttgctgcc taagatgttc ggatgcagga acaggcgggc gttgtcccag tggcgctgcg
    16801 gatcggagta caggtgagtg agcggcgagt caaagaacgg gaacagcagc cgagccacca
    16861 gtgtcagccc gaacagccac ttcactcggc gcgctcttcc ggcaagaaga tccacagcaa
    16921 caaatagacg accagtcccg cgccgaaata gacgaaagtc agaatgaaga tgatgcgcca
    16981 ggcccaggcg ggcaggtcgg tctgcttgcc gaggccgccg cagactccac ccagcatgcg
    17041 atcggtacga gagcgcgcca gctgatacag cagcgcactg ttagaacccg ttcgtgccgt
    17101 ggcctcatcc agcgcctgct gtttggcacg cacgaattcc tgctcgctga tcgtgccgcg
    17161 atcgcgcagc atttgcagct tttccaactc ttccgcgatg ctcatggcta cctccggcag
    17221 gagttagcag aatgccttga ggtcgcgcac ggcgccgtgc agcgtcccgc acaactgcgc
    17281 aaccccgccc aatccgtgcg ctgccagata gcgcgcgatc ccagcgttga tgctctggca
    17341 gaccagcgga tcgtagaaca aagccgtgcc cacgccgacc gtgctggcgc cggcgatgat
    17401 gaattcgatc gcatcgctgg cggtggtgat tccgccctgg ccgatgatcg ggatgcgtcc
    17461 ggggcctgcg acctcgtaaa cctctctgac cttcaacaat gcaatcggtt tgatggccgg
    17521 ccccgacaga ccgccctggg tgttgccgag aaacgctcga ctagtgtcga tgtcgatggc
    17581 catgccggtc acggtattga tgaccgcgaa ggcatcggtg cccgcgtcga tgcacaggcg
    17641 cgcattggtg cgaatgtcgg tctgattggg ggataacttc gtgatcaggg gcttgtcggt
    17701 aacgcgccga caggcttcga ccacacgcgc cgacatctcc ggcacgttgc cgaactgcac
    17761 tccaccctcc ttgatgttgg ggcaggaaat attgatctcg atcgcatcga taggggagtc
    17821 gtcgaagcga cgtgtgacct ccacgtattc ctcgatcgtc gagccgcaaa cattggcaat
    17881 gaagcgcgtc tcgctgaagt caagccgtgg caggatctca cgcaccacgt gatcgacgcc
    17941 ggggttttgc agaccgatcg cattgagcat gccgccgggc gtctcataga cacggtgcgc
    18001 agagttcccg agccgcggtg caccggtggt gcccttcagc accacggcgc cgacgtcgcg
    18061 attgctgaac ccctcgaccc gggtgtactc ctcgccgaag ccgacgcatc ctgacagcag
    18121 cacgatgggg gaggcgaact cgaggccgca gaattggacc gccagtgagg actcgggtcc
    18181 ggatggtttc gccgctacgt ccatgggcat agattatcgc gcatacatag ggtcgacgcc
    18241 gtgtttcatg tcattctcta cgagccggag ataccaccga ataccggcaa tatcatccgt
    18301 ttgtgtgcca ataccggggc cagtctgcat ctggtcgggc cgctggggtt cgacctggag
    18361 cgccggtcgg tcagacgtgc cggtctcgac tacgacgagc tggccagtgt cagccgtcat
    18421 cgcaatctcg cgcagtgcct gaagaggctg cccgatgccc ggctgttcgc cgtcgaaacc
    18481 ggaagtcaca cccgctatgc cgatcgcagc tatcggccgg gggacgcttt cctgttcggc
    18541 cctgaaaccc gcgggctgcc aaccgaggtg ctggcgtcgc tttcagctga gtttcgcatc
    18601 accattccca tgcgccccgg caatcgcagt ctcaatctcg ggaacgccgt ggcggtggtg
    18661 ctgtatgagg cctggcggca gaacggctat cgctcgccaa ccaccagcgt aaaactctgc
    18721 agatagtgaa aatccgggcg acgcagcgcg aaggcgggat cggccggatc gcacaagcgg
    18781 cacagctcag cgtaatcgca ggcttccagg tacgggcgca agcgctctcc ccaggtgtcc
    18841 ctgaaaatcg cctcggtgag gtagatctca tcgacagcgt gcaacggcga aatgcgctcg
    18901 atcatgaacg tgcgcgacca gacattgcgc agcttcgcct ggcgcagcac gccaagcagc
    18961 gcgcgcaccg ccgtgagctc gcgctcggac acgccatagc gctcgcggta atactgccgg
    19021 acggcatcgt tgacgatgcg ctcgagcctc gcatcccagg cgaagaacat ttccggcagc
    19081 agtgagcttt gccccaaggc gatgcgcccg ctgttgcgca gcagtgtgcc ccattgcctg
    19141 atggcttcca ccggctcacg cagatgattg agcgtgttga cacaccacac caggtcgaaa
    19201 tgcgccgcgc gcagcggcag cctcagcgcg tctgcttgag cgatgagaat gtcggccggc
    19261 gaatggcggc gcgcggcctg caggtgggcg gcggccagat ccacgccgac gacgcgacca
    19321 ccgggctgca gcgcggcctg caaccatccg agcgtttcgc ccgtgccgca gccgacatca
    19381 accgcgtgca tgcccggccg cagttgcaga cttgcaatgg cctgccgcaa ctcgggttgc
    19441 gcgcaggcat tgaacaggct gagtttgcga ctgtaatcgc ggcccgcgga atcgccgagc
    19501 agtcccttgg cagtcaccgc gacagctcct aatcgaactc cggcttgatt ggcctcagca
    19561 tcagaccgcg cactacctgt tcgaccgcga gctgttcata gagcacgcca tacaggcctt
    19621 cggcgatcgg catgacgacg ccctcgcggg tggcgacgac gcgtagcgcg cgggcggcct
    19681 gatagccttc aaccacctgt ccgatctctt cgagtgccgc atcgacgctg gcaccgcggg
    19741 ccagtgcaag gccgaaccgc cggttgcgcg aatgatcgtc agtgcacgtc agcaccagat
    19801 cgccgagccc cgccagtccc atgaaagtct cgcggcgcgc gccgagcgcg acaccgagtc
    19861 gcgtcatctc cgtcaggccg cgagtgatca gagcaattcg tgtgttggca ccgaacccga
    19921 ggccgtcgga caggccggct ccgatggcca gcacattctt gacagcaccg ccgacctcga
    19981 cgccggtgat atcggtggag gtgtaa
//
LOCUS       DATMRM010000256         9816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3860424, whole genome shotgun sequence.
ACCESSION   DATMRM010000256 DATMRM010000000
VERSION     DATMRM010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9816
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3860424"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="VNZ06_07515"
     CDS             <1..1243
                     /locus_tag="VNZ06_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW20637.1"
                     /translation="QTIMARLNQSAATISGIKLFLQPVQDLTIDSTVGRSAYQFILEA
                     ASATTLRPWVPKLLDRLQHAPQLSNVSTNFVDAGLTVQIDIDRDTAARFGVTAATIDN
                     ALYDAFGQRIISTIYTQSNEYRVIMEGDPKLQTSIDSLNDIYVPSGTSSSNGQVPLGS
                     IAHISVRSAPLEIDHLGQFPAAMFSFDVDPSASLENAVDAIRQAEKDVGLPAGITTTF
                     EGAALAFQSSLRNEILLILAAIVTVYIVLGVLYESYIHPLTILSTLPSAGIGALLGLM
                     IARLDLNIISIIGIILLIGIVKKNAIMMIDFALTAERDGGKSPQEAIHEAALLRLRPI
                     LMTTVAALFAALPLMLGRGTGSELRAPLGISIVGGLLVSQLLTLFTTPVIYLFFDRLA
                     TRVRGEKPRSYPAALDPEPQS"
     gene            1240..4689
                     /locus_tag="VNZ06_07520"
     CDS             1240..4689
                     /locus_tag="VNZ06_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW20638.1"
                     /translation="MSIATPFIRRPVATTLLTIGLALSGGLAFFLLPVSPMPNVDMPT
                     IVVSASLPGASPETVATSVTTPLERHLGTIAGVTEMTSQSAVGSSRVVLQFELSRDID
                     GAARDVEAAIQAARIDLPSSLRSNPGYRKLNPSDAPVMILALTSDTLTAGQIYDSAST
                     ILQQKLLQVDGIGDVTIGGSSLPAVRIELNPRALFRYGIGLEDVRAALSAANANSPKG
                     AVDFGNRRYQIYVNDTATRAAQYQSLIVAYRNNAGVRLQDVATVTDGVENIRNLGLAD
                     GKPAVLLVLHRQPGANVIDVVDRIRALIPELQASIPASISIIQTNDRTETIRASLRDV
                     ERTLLIAISLVVVVVFLTLRNVRAATVPSVAVPLSLIGTFGAMYLLGFSLDNLSLMAL
                     TVATGFVVDDAIVVLENISRHVEAGMSRFQAALRGAREVGFTVLAMSLSLIAVFIPIL
                     LMGGIVGRTFREFALTLSIAILISLVVSLTTTPMMCAHVLKRGTEQQGRFMNAMERGF
                     SAMRDFYGRTLHDALAHPRTVMVVLAITVALNFYLYTIVPKGFFPQQDTGTLVGNLRA
                     DQSISFQAMSQKLREVQSIVQSDPAVQDVVGFTGGGGGGPGGGGGATNTANLFVGLKP
                     LSQRKASSDRVIMRLRLKLARITGVRLFLQSIQDIRAGGRQSNSQYQYTVLCDDLNLL
                     YQWTPKITEALKSVPELSDINSDRQDNGLDIALNIDRNTAARLGVNLSEIDNTLYDAF
                     GQRQVSTIYNDMNQYHVVMEVDPGFWQSPQTLKEVWVSTSGGALSGTQSSAGALGDFQ
                     PRSGSSSPSAGGSAAPVISSSPGIATLTSASNSGAGAAAGTTGSSGPSTSAATSSLAP
                     SAAAQNLALNSLTNSGRGGASTGASIATSAETTVPLAAFASYGPSTTPLAINHQGPFV
                     ATTFSFNLPEGESLSSAIAAIGRTMQQIHVPISIHGSFAGTAQVFQQSLSNEPWLIVA
                     AIVAVYIVLGVLYESYIHPITILSTLPSAGVGAVLALLAFGTEFSLISLIGVILLIGI
                     VKKNAIMMIDVALETERAREVDPRTAIYDACLLRFRPIMMTTFAALLGATPLAVTTGS
                     GAEMRQPLGISIVGGLIVSQVLTLYTTPVVYLYLDRFRLWMRRSESRRTPSIRHTPQQ
                     LPT"
     gene            4686..6188
                     /locus_tag="VNZ06_07525"
     CDS             4686..6188
                     /locus_tag="VNZ06_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWW20639.1"
                     /translation="MKFKTRSRAQCACTLAIASALAGCAVGPNYHRPPAPLAPAFKES
                     QDWKPATPGQIDSDKPWWSIYSDPLLDSLERQIEVSNQTLKADEAAYRAAVEVVAIDR
                     GSFLPSVSATGSLTRSGSGATGVNQAIATGSGTITAPSTPNSKSTVRNLYSLGAQASW
                     TLDVWGRIRRQVESDVARAQASAADVAAAKLSAQITLASDYFQLRAADEQARLYKTDI
                     EDFQNALTITQNQVKAGITTLADVYAAETQLESTEAAAVNVELTRAKFEHAIAVLTGK
                     PPSELTIPDGPFVTVVPVIPSGVPSSLLERRPDIAAAERDVASANALIGVAEAAWFPT
                     LTLSGSYGFTSTVLTGLISAHNSLWSFGPALAENLFNGGATLAQTREARANYDQAVAT
                     YRQTVLSAFQSVEDDLASLRVLEHQGELQQTAVRDARSSEALTLNQYKSGIVAYSSVI
                     TSQTTRLADEISLLSIQSQALVSSVDLISQLGGGWDAAQLNRQDRGVPSK"
     gene            6257..6637
                     /gene="crcB"
                     /locus_tag="VNZ06_07530"
     CDS             6257..6637
                     /gene="crcB"
                     /locus_tag="VNZ06_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HWW20640.1"
                     /translation="MSVAIGAALGALLRWRLGVWLNPIFPTIPFGTLASNLLGGFVVG
                     LCMEYFARNAAVPPELRLAAVTGFLGGLTTFSTFSAETTALLLRRDYGWSLTLIAAHL
                     IGSLLLTIAGIYCVRALFSRGLSL"
     gene            complement(6634..>9816)
                     /locus_tag="VNZ06_07535"
     CDS             complement(6634..>9816)
                     /locus_tag="VNZ06_07535"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWW20641.1"
                     /translation="VSSIFCEAGVVTRRVAVAAEKAGVVFAVDPTSADASCIGGNIAM
                     NAGGKKAVLWGTAVDNLAWWRMVDPQGNWLEVTRLGHNLGKIHEVEFAEFELIWKDGE
                     QSPDRARVLRQERLSIPGQRFRKVGLGKDVTDKFLGGLPGVQKEGCDGVITSARWILH
                     RSRAFTRTFCLEFFGQARDAIPAIVEIKQHLDAQAARGVYLAGLEHMDERYLRAVGYG
                     TKSKRGALPKMALFGDVVGDDENAVAVTTSDVVRIANARNGEGFIAVSAEARRKFWLD
                     RARTAAIARHTNAFKINEDVVIPLDRMGDYTDAVERINIEFSLQNKLRLAEELAQLLD
                     SPLPLGRSEDGESTEIPTESRVGERVAQAQQLLAGVNTRWKWLLQHLDLRLAEAVTQL
                     EAIGLEWLLPEIHGRLQRNAASTVFDLVQDRSIRVSWKRELKAPLEQIFVGRAYAPTL
                     SACQQLHRRILRSRLFVALHMHAGDGNVHTNIPVNSDDYDMLQAASAAVRRIMAIARS
                     LGGVISGEHGIGITKLEFLQPAETLDFRTYKYRIDPEGRFNRGKLLPGGDLSNAYTPS
                     FNLLGHESLIMKQSDIGSISDAIKDCLRCGKCKPVCATHVPRANLLYSPRNKILATSL
                     LIEAFLYEEQTRRGVSVRHWDEFADVGDHCTVCHKCEAPCPVDIDFGDVSMAMRDLLR
                     RMGKKRFNPGSAAAMFFLNATNPQTVRVARKLMIDWGYKAQRTANQLLRGLARAQTSH
                     PPATTGKAPLREQLIHFVNKKMPGGLPKKTARALLDIENPDYVPIIRNPALTGGDTEA
                     VFYFPGCGSERLFSQVGLATQAMLWHVGVQTVLPPGYLCCGYPQRGAGQFDRAEQIQT
                     NNRVLFHRVANTLNYLDIRTVVVSCGTCYDQLLGYKFEEIFPGCRIIDIHEYLMEKNV
                     RLEAVTGARYMYHDPCHSPMKTYDPLKVVNTLMNERDNGHIARNERCCGESGTLALTR
                     PDVSTQVRFRKEQELRAGASTLRDAGATQVKVLTSCPSCLQGLKRYNADAELDAEYIV
                     VEIARHVLGANWMVEFVARANNGGIERVLV"
BASE COUNT         1769 a         3198 c         2992 g         1857 t
ORIGIN      
        1 gcagacgatc atggcacggc tgaatcaatc cgcggccacc atcagcggca tcaagctgtt
       61 cctgcagccg gtgcaggacc tgacgatcga ctccacggtc ggacgatcgg cgtaccagtt
      121 catcctcgag gccgccagcg ccacgacgct gcgtccctgg gtgccaaagc tattggatcg
      181 cctgcagcac gcgccgcagc tcagcaacgt gtccaccaac ttcgtcgacg ccggtctgac
      241 cgtgcagatc gacatcgatc gtgataccgc agcacgcttt ggcgtgaccg cggcgacgat
      301 cgacaacgct ctgtacgacg cgttcggtca acgcatcatc tccacgatct atacgcagtc
      361 gaacgagtat cgggtgatca tggagggtga tccgaaactg cagacctcga tcgattcact
      421 gaacgacatc tacgtaccgt ccggcacctc atcgagcaat ggtcaggtac cgctgggatc
      481 gatcgcgcac atcagcgtgc gttcggcacc gctggagatc gatcatctcg gacagtttcc
      541 ggcagcgatg ttctcgttcg acgtcgatcc atcggcctca ctggagaatg ccgtcgacgc
      601 catccgccag gcagagaagg acgtcgggct tccagccggc atcacgacga ccttcgaggg
      661 cgccgcgctg gcattccagt cctctttgcg caacgagatc ctgctgatcc tggccgccat
      721 cgtgacggtc tacatcgtgc tcggcgtgct atatgagagt tacattcatc cgctcaccat
      781 tctgtcgacg ctgccgtcgg ccggtatcgg agcgctgctc ggattgatga tcgcgcggct
      841 cgacctgaac atcatttcga tcatcggcat catcctgctg atcggcatcg tcaagaagaa
      901 tgcgatcatg atgatcgact tcgcgctcac ggcggagcgt gacggtggca agagcccaca
      961 ggaagcgatt cacgaggccg cgttgctgcg cttgcggccc attctgatga ccaccgtggc
     1021 ggcactgttc gctgccttgc cgctgatgct gggccgaggc accggttcgg agctgcgcgc
     1081 accgctgggt atctccatcg tgggcgggct gctggtcagt cagctgctga cgctgttcac
     1141 tacgccggtc atctatctgt tcttcgaccg gctggcaacc cgtgtgcgcg gtgagaagcc
     1201 ccgcagctat ccggcggcgc tggatcctga gccgcagtcg tgagtatcgc gacacctttc
     1261 atccgccgac cggtggccac gacgctgctg acgatcggcc tggcactgtc cggcggactg
     1321 gcattcttcc tgctgccggt ctcaccgatg ccaaacgtcg atatgccgac gatcgtcgtc
     1381 tcggcctcgc tcccgggcgc gagcccggag accgtcgcga ccagtgtcac aacgccgctg
     1441 gaacggcact tgggcacgat cgccggggtc accgaaatga cttcgcagag tgccgtgggc
     1501 agttcccggg tggtactgca attcgagttg tcgcgcgaca tcgacggcgc cgcacgtgac
     1561 gtcgaggcgg ccattcaggc cgcgcgcatc gatctgccga gttcactgcg cagcaatccc
     1621 ggctatcgca agctcaatcc ttccgatgcg ccggtgatga tcctggcgct gacctccgac
     1681 acgctcaccg ccgggcagat ctacgattcc gcatccacga tcctgcagca gaagctattg
     1741 caggtggacg ggatcggcga tgtgaccatc ggcggcagct cgctgcccgc ggtgcgcatc
     1801 gaactcaatc cgcgcgcgct gttccgctat ggcatcggcc tcgaggacgt gcgcgccgca
     1861 ctgtccgccg ccaacgccaa cagccccaag ggagcggtcg actttggtaa tcgccgctac
     1921 cagatctacg tcaacgacac cgcgacgcgc gcggcgcagt accagtccct gatcgtcgcc
     1981 taccgcaaca acgccggcgt gcgcctgcag gatgtcgcca ccgtcaccga cggcgtcgag
     2041 aacatccgca atctcgggct ggcggacggt aaaccggcgg tactgctggt gctgcaccgt
     2101 caaccgggcg ccaatgtcat cgacgtggtg gaccgcatcc gggcactgat tccggagctg
     2161 caggcctcga ttccggccag tatcagcatc atccagacca atgatcgcac cgaaaccatc
     2221 cgcgcctcgc tgcgcgatgt cgagcgtacg ttgctgatcg ccatttcgct ggtggtggtc
     2281 gtggtgttcc tgacgctgcg caatgtgcgc gcggcgaccg tgccaagcgt cgccgtgccg
     2341 ctgtcgttga tcggcacgtt cggcgcgatg tatctgctcg gcttcagtct cgacaacctg
     2401 tcgctgatgg cgctcacggt cgcaaccggg tttgtggtcg acgatgcgat cgtggtgctg
     2461 gagaacatct cacgtcatgt cgaagcaggc atgtcgcgct tccaggctgc actgcgcggt
     2521 gcgcgcgaag tgggcttcac cgtgctggcg atgagcctgt cgctgatcgc agtattcatc
     2581 ccgatcctgc tgatgggcgg catcgtcggg cgcacctttc gcgagttcgc actgacgctg
     2641 tcgatcgcga tcctcatctc actggttgtc tcgctgacca cgacgccgat gatgtgcgca
     2701 cacgtgctga agcgcggtac cgagcagcag ggccgtttca tgaatgcgat ggaacgcggc
     2761 ttcagcgcca tgcgcgattt ctacggccgc accttgcacg atgcactggc gcacccgcgc
     2821 accgtgatgg tggtgctggc gatcacggtc gcgctcaatt tctatttgta taccatcgtg
     2881 ccgaaaggct tcttcccgca gcaggacacc ggcacgctgg tcggcaatct gcgggccgat
     2941 cagagcatct catttcaggc gatgtcacag aaactgcgtg aagtgcagtc gatcgtgcaa
     3001 agtgatccgg cggttcagga cgtggtcgga ttcaccggcg gcgggggtgg tggtccgggc
     3061 ggcggtggcg gtgcgaccaa tacggccaac ctgttcgtcg ggctcaagcc gctgagtcag
     3121 cgcaaagcct cgagcgatcg cgtcatcatg cgactgcggc tgaaactcgc gagaattacc
     3181 ggggtacgtc tgttcctgca atcgatccag gatatccgcg ccggcggccg ccagagcaac
     3241 tcgcagtacc aatacacggt cctctgcgat gatctgaacc tgctctacca gtggacgcca
     3301 aagatcaccg aagcactgaa gagcgtgccc gaactgtcgg acatcaactc ggatcgacag
     3361 gacaacggac tggacattgc cctgaacatc gatcgcaaca ccgcggcgcg cctgggtgtg
     3421 aatctctccg agatcgacaa cacgttgtat gacgcgttcg gccagcgcca ggtctccacg
     3481 atctacaacg acatgaatca gtatcacgtg gtgatggaag tcgatccggg cttctggcag
     3541 agcccgcaga ccctgaagga ggtctgggtc agcacctcgg gcggtgcgct cagcggaacc
     3601 cagtcctcgg caggcgcgct gggtgatttc cagcccagat cgggcagctc gtcaccgagc
     3661 gctggcggca gcgcggcccc ggtgatcagc tcgagcccgg gcatcgcgac gctgacgtcc
     3721 gcgagcaaca gcggtgccgg cgcggcggcc ggtaccacgg gctcgagcgg cccttccacg
     3781 agcgcggcga cctcgagcct cgcgccatct gcggccgcac aaaacctggc cctgaactcg
     3841 ctcaccaaca gcgggcgggg tggggcctcc accggcgcct cgatcgcaac ttccgccgag
     3901 accaccgtgc cactggcggc atttgccagc tacgggccga gcaccacgcc gctcgcgatc
     3961 aatcaccaag gcccgttcgt ggcgaccacg ttctcgttca atcttccgga aggcgaatcg
     4021 ctcagcagcg ccatcgccgc catcgggcgc acgatgcaac agatccacgt tccgatctcg
     4081 atccacggct cctttgccgg caccgcacag gtgttccagc agtccctgag caacgagccg
     4141 tggctgatcg tcgccgcgat cgtcgcggtc tacatcgtgc tcggcgtgct gtacgaaagc
     4201 tacattcatc cgatcaccat tctgtcgacg ctgccttccg ccggcgtcgg cgcggtactc
     4261 gcgttgctgg ccttcggcac cgagttcagc ctgatctcgt tgatcggcgt catcctgctg
     4321 atcgggatcg tcaagaagaa cgcgatcatg atgatcgacg tggcactgga aaccgaacgc
     4381 gcgcgcgagg tcgatccccg caccgccatc tatgatgcct gtctgctacg cttccggccg
     4441 atcatgatga ccacgttcgc agcgctgctg ggagccactc cgctggcggt gacgactggc
     4501 agcggtgccg aaatgcggca gcccttggga atatcgattg tcggcggcct gatcgtcagt
     4561 caggtgctga cgctctacac caccccggtg gtgtatctgt acctggatcg cttccgactg
     4621 tggatgcgtc gctccgaatc acgccgtacc ccatcgatcc gccatacgcc acaacagttg
     4681 cccacatgaa gttcaagacc cgctcgcgcg cccagtgcgc ctgcacgctg gcgatcgcat
     4741 cggccctcgc aggttgtgcc gtcggtccga actatcatcg ccccccggca cccttggccc
     4801 cggccttcaa ggagagccag gattggaaac cggcaacgcc ggggcagatc gactcggaca
     4861 agccgtggtg gtcgatctac agcgatccac tgctcgattc actggagcgg cagattgaag
     4921 tctcgaacca gacactgaag gccgatgaag cggcctatcg cgccgcagtg gaagtcgttg
     4981 cgatcgatcg cggctcgttt ctgccgagcg tgtcggccac gggctcactg acccgctccg
     5041 gcagcggtgc cacgggagtc aatcaggcca tcgcgaccgg gtcgggcacg attaccgcgc
     5101 cgagcactcc caacagcaag agcacggtgc gcaatctcta cagcctcggc gctcaggcga
     5161 gctggacact cgatgtctgg ggtcgcatcc gccggcaggt cgagtctgat gtcgcccggg
     5221 cgcaggccag tgccgcggac gtcgccgcgg ccaaactgtc cgcacagatc accctggcct
     5281 cagactactt tcagctgcgc gcagccgacg agcaggcgcg tctgtataag accgatatcg
     5341 aagatttcca gaacgctctg accattacgc agaaccaggt gaaggccggc atcacgacgc
     5401 tggccgacgt ttacgccgcc gagacgcagc ttgaaagcac cgaggccgcg gcggtcaatg
     5461 tggaactgac tcgcgccaaa ttcgaacacg cgatcgcggt gctcacgggc aagccccctt
     5521 ccgaactcac gatcccggat ggtccattcg tcacggtggt tccggtcatt ccgtccggcg
     5581 tgccctcctc gctgctcgaa cggcgaccag acatcgccgc cgccgaacgc gacgtcgcca
     5641 gtgccaacgc gctgatcggg gtcgctgaag cggcctggtt cccaaccctg acgctcagtg
     5701 gcagttacgg tttcacgtcc accgtgctga cgggtctgat cagcgctcat aactcgctgt
     5761 ggtcatttgg gccggcgctg gccgagaatc tgttcaacgg cggcgccacg ctcgcgcaaa
     5821 cccgcgaggc gcgtgccaac tatgatcagg cggtcgctac ctatcgccag accgtgctgt
     5881 cggcctttca gtcggtcgag gacgacctgg cgagcctgcg ggtactggag catcagggcg
     5941 agttgcagca gaccgcggtc cgggatgcgc gctcatcgga agccctcacg ctcaatcaat
     6001 acaagtccgg catcgtcgcc tactcgagcg tgatcacatc gcagactacc cgcctggccg
     6061 atgagatcag tctgttgagc atccagagcc aggcgctggt atcgagcgtg gatctgatct
     6121 cgcagctcgg aggcggctgg gacgccgcgc agctgaatcg tcaggatcgc ggcgtgccgt
     6181 cgaagtaggc gaagcgttgc acttgccaaa tgcccccgca atccgggagc atccctgacc
     6241 catgatcggg cagggaatgt ctgtagctat cggcgcggcg ctcggggcgt tgctgcgctg
     6301 gcgtctgggc gtttggctca atccgatctt tcctaccatc ccgttcggca cgctggcgtc
     6361 gaatctgctg ggcggattcg tggtcggtct gtgcatggaa tacttcgcgc gcaatgcagc
     6421 ggttccgccc gagctgcgcc tggcggcggt cacggggttt ctgggcggcc tgacgacctt
     6481 ctcaaccttc tcggccgaga ccacggcgct gctgctgcgg cgcgactacg gctggtcgct
     6541 caccctcatc gccgcgcatc tgatcggatc attgctgctg acgatcgccg gcatctactg
     6601 cgtgcgcgcg ctcttcagcc gcggcctgtc gctctaaacc agcacccgct cgatgccgcc
     6661 gttgttggca cgcgcgacga attccaccat ccagttggcg cccagcacgt ggcgcgcgat
     6721 ctccaccacg atgtattcgg catcaagctc cgcatcggcg ttgtagcgtt tgagcccctg
     6781 aaggcacgac gggcaggagg tcagtacctt gacctgcgtg gcgcccgcgt cgcgcagcgt
     6841 ggaagcgccc gcgcgcagct cttgctcctt gcgaaaacgc acctgagtcg agacatcggg
     6901 ccgggtcagt gccaaggtgc ccgactcacc gcagcagcgc tcgttcctgg caatgtggcc
     6961 gttgtcgcgt tcattcatca gcgtgttcac caccttgagc ggatcgtaag tcttcatcgg
     7021 cgagtgacac gggtcgtgat acatgtagcg ggcacccgtg acagcttcga gccgaacgtt
     7081 cttctccatc agatactcat gaatatcgat gatgcggcag cccggaaaga tctcctcgaa
     7141 tttgtagccg agcaactggt cgtagcaggt accgcagctc acgaccacgg tgcggatatc
     7201 cagatagttg agggtgttgg ccacccgatg gaacaacacc cgattgttgg tctgtatctg
     7261 ttcagcacga tcgaactgtc cggcaccgcg ctgtggatag ccgcagcaca ggtagcccgg
     7321 cgggagcacc gtctgaaccc ccacatgcca caacatcgcc tgagtggcca accccacctg
     7381 tgaaaacagg cgctccgagc cgcagcccgg gaaatagaac accgcctcgg tatcaccacc
     7441 ggtcagggcg ggattgcgaa tgatcggcac gtagtccgga ttctcgatgt ccagcaatgc
     7501 gcgcgcggtc ttcttcggca aacctcccgg catcttcttg ttgacgaaat ggatcagctg
     7561 ctcgcgcagc ggcgccttgc cggtggtggc cggcggatgc gacgtttgcg cacgggccag
     7621 tccgcgcagc agctggttgg cggtgcgctg cgccttatag ccccagtcga tcatgagttt
     7681 acgcgccacg cgcaccgtct gcggattggt ggcattgagg aagaacatcg ctgccgccga
     7741 gcccggattg aagcgtttct tccccatccg gcgcagcaga tcgcgcattg ccatcgagac
     7801 atcgccgaaa tcgatgtcca ccgggcaagg cgcctcgcac ttgtgacaga ccgtgcagtg
     7861 atcgccgacg tcggcgaact catcccaatg acgtacgctg accccgcgcc gggtctgctc
     7921 ctcgtacagg aatgcttcga tcaggagcga ggtggcgaga atcttgttgc gcggactgta
     7981 gagcaggttg gcacgcggca cgtgcgtggc acacaccggc ttgcatttgc cgcaacgcag
     8041 acagtccttg atggcatcgg agatcgaacc gatgtccgac tgtttcatga tcagtgattc
     8101 gtggccgagc aggttgaaac tcggcgtata ggcattcgac agatcgccgc ccggcagtag
     8161 tttgccgcga ttgaaccgtc cctcggggtc gatacggtac ttgtaggtgc ggaagtcgag
     8221 tgtctcggcg ggctgcagga actcgagctt ggtgatgccg ataccgtgtt caccggagat
     8281 cacgccaccc agactgcgcg cgatcgccat gatgcgtcgt accgcggcgc tcgcggcctg
     8341 gagcatgtcg tagtcatccg agttcaccgg aatattggta tgcacgttgc cgtcgccggc
     8401 atgcatgtgt agggcgacga acaggcggct gcgcaaaatc cgacgatgca gctgctggca
     8461 tgcgctcaag gtcggggcat aggcgcgacc gacaaaaatc tgttccagcg gcgccttgag
     8521 ttcgcgcttc cacgagacgc gaatactgcg gtcctgcacc agatcgaaca ccgtggaggc
     8581 ggcattgcgt tgtagccggc cgtgaatctc gggtagcagc cactccaagc cgatcgcttc
     8641 cagctgcgtc actgcctcgg ccagcctcag gtccagatgc tgtagcagcc acttccatcg
     8701 ggtattgacc ccggcgagca gttgctgcgc ctgtgcgacg cgctcgccca ctctgctttc
     8761 agtgggtatt tccgtgctct cgccatcctc ggaccgaccg agcggcagcg gcgaatccaa
     8821 cagctgcgcg agctcctccg ccaggcgcaa cttgttctgc agtgaaaatt cgatgttgat
     8881 gcgctccacg gcatcggtgt agtcgcccat gcgatcgagc gggatcacga cgtcttcgtt
     8941 gatcttgaag gcgttggtgt gccgggcaat cgcggcggtg cgagcgcggt ccagccagaa
     9001 cttccggcgc gcctccgcac tcacggcgat gaagccctca ccgttgcgag cattggcgat
     9061 gcgcaccacg tcagaggtgg tgaccgctac cgcattctca tcgtcgccaa cgacatctcc
     9121 gaacagcgcc attttgggca aggcaccacg cttggacttg gtgccatagc ccaccgcgcg
     9181 cagatagcgc tcgtccatgt gctccagtcc ggccagatac acaccgcgcg cagcctgcgc
     9241 atccaggtgt tgcttgattt ccacgatcgc ggggatggcg tcgcgggcct gaccgaagaa
     9301 ctccaggcaa aaggtgcgcg tgaaggcgcg gctgcgatgc agaatccagc gcgcagaggt
     9361 gatgacgccg tcacaaccct ctttctggac gccgggtaag cctcccagga atttatcggt
     9421 aacgtcctta ccgagcccta ccttgcgaaa gcgctgaccg gggatggaca ggcgctcctg
     9481 gcgcagcact cgggcgcgat ccgggctttg ttcgccgtcc ttccagatca gttcaaattc
     9541 ggcgaactcg acctcatgga tctttcctag gttgtgtccg aggcgtgtga cttcgagcca
     9601 gttgccctgt gggtcgacca tgcgccacca cgcaagattg tccactgctg tgccccacag
     9661 caccgccttt ttgccgccgg cattcatcgc gatgttgccg ccgatgcagg atgcatcagc
     9721 ggatgtggga tcgaccgcga ataccactcc ggctttttcc gccgccaccg cgacccgccg
     9781 cgtcacgacg cccgcctcgc agaagatgct ggatac
//
LOCUS       DATMRM010000257         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1385573, whole genome shotgun sequence.
ACCESSION   DATMRM010000257 DATMRM010000000
VERSION     DATMRM010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385573"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VNZ06_07540"
     CDS             <1..608
                     /locus_tag="VNZ06_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWW20642.1"
                     /translation="QGGSAEALAKDSPELESRGDEVVRIIGSEHGRLVPRYYATLIAG
                     IRSASTQVWVTAAYFVPTFQERQALARAARRGIDVRLLLPSHSDSSPALAVQQSYYAG
                     LLHAGVKIFEREDGILHSKTIVTDSVWSMVGSSNFDHRSVLFNDEVDAVVIGRKTGAA
                     LEQYFSDGQEHARQVDLKSWQERPFLHKLRERFWRLWEVWL"
     gene            complement(618..1181)
                     /locus_tag="VNZ06_07545"
     CDS             complement(618..1181)
                     /locus_tag="VNZ06_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20643.1"
                     /translation="MLIARGPALVLSTLMAGCIVSVAVSADTAVTPATEAGNWTAHKY
                     TFNFMGFTTTYSCEGLEDKLKALLQMNGAGPDKHDVQVVAPCQNQIGRPDKLAFAYLT
                     FSSLQPGSAGSASAGEWKHVTLAPRHPFQFDYGDCELVEQFRDKVLPMFTTRNLTNNV
                     TCVPHQDTGSNYYLSYDVFAPPTPVKR"
     gene            complement(1224..>2176)
                     /locus_tag="VNZ06_07550"
     CDS             complement(1224..>2176)
                     /locus_tag="VNZ06_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWW20644.1"
                     /translation="AVITSSGMAAVTLCLQVLPSRARIVAPHDCYGGSYRLFDALHRR
                     EDLRVEFVNFADLEALRAALSKPTGLVWIETPSNPLLRLTDIAAVAQLGHAAGALVVV
                     DNTFLSPAWQQPLKFGADLVVHSTTKYLNGHSDVVGGAVVAANQEHGQQLGWWANCLG
                     LTGSAFDSYLTLRGLRTLHARLAIHGSNALALASWLTQQKGVSRVYYPGLPTHPGHEL
                     ARRQQTGFGAIVTFELASGIPDVKRFVAGLKYFSLAESLGGVESLIAHPASMTHAAMH
                     ETARRAAGLTDGLLRLSVGIESVEDLRADLAAGLSRCVNC"
BASE COUNT          402 a          711 c          687 g          376 t
ORIGIN      
        1 cgcagggcgg cagcgcggag gcgctggcga aggattcgcc cgagctggaa tcccggggcg
       61 atgaggtggt gcgcatcatc ggcagcgagc acggccggct ggtgccacgt tactacgcta
      121 cgctcattgc cggcatccgc agcgccagca cccaggtgtg ggtcactgcc gcctatttcg
      181 ttccgacctt ccaggagcgg caggcgctgg cacgtgccgc gcggcgcggc atcgatgtgc
      241 gcctgctgct gccctcgcac agtgactcct ctccggcact ggccgtgcag cagtcctatt
      301 acgccggtct gctgcacgcg ggggtcaaga tcttcgagcg cgaggacggg atcctgcatt
      361 cgaagaccat cgtcaccgac agcgtctggt cgatggtcgg ctcatcgaat ttcgatcatc
      421 gcagtgtact gttcaacgac gaggtcgacg cggtcgtgat cgggcgcaag accggcgcag
      481 ccctcgagca gtatttcagc gacggccagg agcatgccag gcaggtggat ctgaagagct
      541 ggcaggagcg gccgttcctg cacaagctgc gcgaacggtt ctggcgcctg tgggaggtat
      601 ggctgtgaac gaagtcgcta gcgcttgacc ggcgtcggcg gcgcgaacac gtcgtaactc
      661 agatagtagt tcgaaccggt atcctgatgc ggcacacagg tgacgttgtt ggtgagattg
      721 cgggtggtga acatcggcaa caccttgtcg cggaattgtt ccaccagctc gcaatcacca
      781 tagtcgaact gaaagggatg gcgcggagcg agtgtcacgt gcttccactc cccggccgag
      841 gcgctacccg cgctgcccgg ctgcagactc gagaacgtaa gataggcgaa cgccagcttg
      901 tccggccgcc cgatctggtt ttgacacggc gccacgacct gcacatcgtg cttgtcggga
      961 ccggcgccat tcatctgcag caacgctttc agcttgtctt ccaggccttc acacgagtac
     1021 gtcgtggtaa agcccatgaa gttgaacgtg tacttgtgcg ccgtccagtt gccggcttca
     1081 gtagcgggtg tcaccgcggt gtcggccgac accgccaccg acacgatgca ccctgccatc
     1141 agcgtactca ggaccagcgc tggcccgcgt gcgatcaaca taggtccacc tcgcgtagag
     1201 ctaagcataa cgcaccgtca gcgttaacag ttgacacagc gcgacaggcc ggcggccaga
     1261 tccgcgcgca gatcctccac cgattcgatg ccgaccgaca gtcgcagcaa cccgtcggtc
     1321 aggccggccg cgcgacgtgc ggtttcgtgc atcgctgcat gggtcatcga tgccggatgt
     1381 gcaatcagac tctccacacc gcccagtgat tctgcgagcg aaaaatactt gagcccggca
     1441 acaaatcgct tcacatccgg gatgccgctc gccagttcaa aggtcacgat ggcaccgaat
     1501 ccggtctgct gacgcctggc cagttcgtgg cccggatgcg tcggcaggcc ggggtagtac
     1561 accctggaca cacccttctg ctgcgtcagc cagctcgcca gtgcgagcgc gttgctgccg
     1621 tgaatggcca gccgggcgtg cagcgtacgc agcccgcgca gcgtcagata gctgtcgaat
     1681 gcggagccgg tgagtcccag gcaattggcc caccagccca gctgctgccc gtgttcctgg
     1741 ttcgccgcga ccaccgcgcc gccgaccaca tcgctgtgac cattgagata tttggtggtc
     1801 gaatgcacca ccagatcagc gccgaacttc agcggctgct gccacgcggg cgacaggaac
     1861 gtgttgtcga ccaccaccag cgcgccggcg gcgtggccca gttgcgccac cgcggcgatg
     1921 tcggtcaggc gcagcagcgg attgctgggc gtctcgatcc acaccagtcc ggtcggcttt
     1981 gacagtgccg cgcgcaacgc ctccagatcg gcgaaattca cgaattcgac ccgcaggtcc
     2041 tcgcgccgat gcagcgcgtc gaacagccga tacgaaccgc cgtagcagtc gtgcggagca
     2101 acgatgcgcg cgcgcgaagg cagcacctgc agacacagcg tcaccgccgc catgccggag
     2161 ctcgtgatca ccgcac
//
LOCUS       DATMRM010000258        10099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1717256, whole genome shotgun sequence.
ACCESSION   DATMRM010000258 DATMRM010000000
VERSION     DATMRM010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10099
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717256"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1436
                     /gene="serS"
                     /locus_tag="VNZ06_07555"
     CDS             147..1436
                     /gene="serS"
                     /locus_tag="VNZ06_07555"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWW20645.1"
                     /translation="MIDPKLLRSAPEEIARQLARRGYQLDVTAWQRLEERRRHGQIET
                     DRLRAARNAHAKSVGQARVRGEDIAPLMQQGEQLAKELAGAEQEFNGVQAELDRWQLD
                     IPNLPHESVPAGTDESANVEVARSGEPRQFSFKPRDHVELGERLGALDFEAASRISGT
                     RFVVIRGEVARLQRALAQYMLDLHTRAHGYTEIYAPYLVLPHALVGTGQLPKFEQDLF
                     AVRGEPGFYLIPTSEVSMTNLVREQIIAAEALPLKFTAHTPCFRSEAGAAGRDMRGMI
                     RQHQFEKVELVQIVRPADSYAALENLRSNAETVLQKLELPYRVVALCGGDMGFTAAKT
                     YDLEVWLPSQEKYREISSCSNCEAFQARRMQARWRNPESGKPELLHTLNGSGVAIGRA
                     LVAVLENYQQADGSVEVPSVLRPYMGGVALIGKGIRS"
     gene            1453..2679
                     /locus_tag="VNZ06_07560"
     CDS             1453..2679
                     /locus_tag="VNZ06_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HWW20646.1"
                     /translation="MEPSAPGKTRRFALPLQIVLAVILGVVVGLLWPKTGAAMQPLGT
                     AFINAVKMIVIPIVFCAVTLGAMKMGRDAPRLGRMALIAFVWFYFSSAVAIAIGLILD
                     TLFHPGLGIHLDVTGPAPSQLTNGIDWVKFLLELIPSNIVSAMANQKVLPTLLFAVLF
                     GLGLSAVGEPRAKPMADVLESVLAVMFRVTNWIVSLAPIAVFGVVAWLFASQGSATVW
                     ALARLIGVAYLGYTIFGLLCVLGFAWLGEPPIALTRAMSGPTLLGFVTRSSEASLPLH
                     MEKLEQWGIPNRIVSVILPLGYSFNLAGAALYIGLATTFIAEAYGLRLALPQLLTVLA
                     TALIASKGVANVPSASLIAISTVLLALGIPADAIAIIAGVDVFLDMGRTAINVFGNTL
                     SVLVARRYGEGEGTGG"
     gene            2672..3457
                     /gene="deoC"
                     /locus_tag="VNZ06_07565"
     CDS             2672..3457
                     /gene="deoC"
                     /locus_tag="VNZ06_07565"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HWW20647.1"
                     /translation="MVDASYTTRMQFTARRVLSLTDLTSLNDNDDERSIQKLLKLAST
                     DAGKVAAVCTWARFIAVAKPILARTGIPLAVVANFPAGAADPVAAAAQTTAAVTAGAN
                     EIDVVFPYRALMSGDEQSGLNLVEACRAACGEQTLLKVILETGQLGSDRNISTAARIA
                     CDGGADFLKTSTGKTQPGATIEAVGVLLDVIAMYAKRGRKVGLKVSGGVRTVEQAKQY
                     LDLYEDYLGVGAATPATFRIGASSLVSDILTVLRPNERDVQQS"
     gene            3540..3629
                     /locus_tag="VNZ06_07570"
     tRNA            3540..3629
                     /locus_tag="VNZ06_07570"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3574..3576,aa:Ser,seq:tga)
     gene            complement(3658..3945)
                     /locus_tag="VNZ06_07575"
     CDS             complement(3658..3945)
                     /locus_tag="VNZ06_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20648.1"
                     /translation="MKTGKLKMKHMLIAGIAVLASACASYKVVPMGQMAYEVQFPAKS
                     CVWCGADDNAVKVATSFCSEQREEAAVIPITPMNWSNSEPGPFVFECIPRG"
     gene            4108..4305
                     /locus_tag="VNZ06_07580"
     CDS             4108..4305
                     /locus_tag="VNZ06_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20649.1"
                     /translation="MALAASLCLFNRGMAIGVGSAMEDKLDRTGDPDCLFRKARTFLK
                     NLPAEFRGGWDETRHTAHMRF"
     gene            complement(4550..5044)
                     /locus_tag="VNZ06_07585"
     CDS             complement(4550..5044)
                     /locus_tag="VNZ06_07585"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWW20650.1"
                     /translation="MKNRTTVERKSERELVVTRTFNGPARMVFEAWTKPELLKRWWAP
                     KSFGVSFLSCEADVRTGGTYRFVFGHAASEQPMAFFGRYIEVTPHSRVVWTNDEGGEG
                     GAVTTVTFQERGDQTLVVMHDLYPSKEALDAAIASGSTGGTGESFEQLDELLVTLGAS
                     AGRS"
     gene            complement(5051..5416)
                     /locus_tag="VNZ06_07590"
     CDS             complement(5051..5416)
                     /locus_tag="VNZ06_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWW20651.1"
                     /translation="MVQYIRACFDASFAALSDATRRGVLEQLGRADASITELAEKFHM
                     TLTGMKKHVGVLEQAGLVTTKKVGRVRTCKLGLRRLEEEAAWIERYRQLWDARFDELD
                     KVVEELKRKEKVDGRKKRE"
     gene            complement(5770..6114)
                     /locus_tag="VNZ06_07595"
     CDS             complement(5770..6114)
                     /locus_tag="VNZ06_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20652.1"
                     /translation="MRRFLAQSARLSRYQYISRETEAARYIARTTQGAFLWYLLAPRQ
                     WREQMSQGSHSHGKTEKVVDLFSSDDPYNRRPIHVTDTARRRTLDDMRRLSEEIKSER
                     AASGRNQVDSRS"
     gene            complement(6256..8718)
                     /locus_tag="VNZ06_07600"
     CDS             complement(6256..8718)
                     /locus_tag="VNZ06_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWW20653.1"
                     /translation="MLTTRSYGIALLTLVLCAWPTPRASAADAGAARPRAALVIGNAA
                     YSAVTALKNSAYDAHDMCDALTELGYTVSCYVDVKDAREFKARIQDFAAALKPKSEVL
                     FYYAGHAVQLKGENYLVPTSAKLRAETDVVRETVSLNYIMTQLLQAKHYLNIVILDAC
                     RSNPWADNPRGMSSGLAPITAIPRGTMVLYATAANDVSDDGEGRNGTLTKNLLANIKI
                     PGLTVDEMFKRVSEGVQGDSAAAVGHTQTPALYTNFTGEFCFAGCIDKVARAELEKMQ
                     KANDEQLEKARQEKAELEAHNREVEAHNREMEARLAATVTSMNCDKTVLNDVGRCFSA
                     TPEQTLRAVTMTLMQRGFRIANSSIEAGYIDAMRSTDDPHDKKLTDVLSATVNVKPIP
                     TTDHSVVTLTAIERTVLHDEFHHWGTLAIIPIPTSKEYKDVVKKEVNVTDSAFYGEFF
                     DAVDRNLRSEASAGVAPAAQPAGAASGDATAAPGDTKDDAPATTNDEAARAVPVTLQT
                     PAPLSTPADTDPGASGNGQTYSASVPQTRMALIQTLVQRGFIIQSVSAELGLINAVYA
                     LQDPKNESYSTDTALTASASPTSSPTIARVQITASEQRLLHRKPGSRIAAATFGYQPR
                     DYENVVVKEGPVNDQGFYRDLLAGIDTNLRGGDAPKASHQQHFATTLENLLTAANDGL
                     AQKGFTITKLDRNLGLILANRRTTAKEGHGWAANYVNVSVVVNSDDAGARLAASASES
                     EAHSSLTSLYGISRRQMNALAGVLGSSACSTGDCSPDQMASLMATVSGGQNSEVISKE
                     GPANAAFFSDLFSTVEQKLAHR"
     gene            complement(8749..9690)
                     /locus_tag="VNZ06_07605"
     CDS             complement(8749..9690)
                     /locus_tag="VNZ06_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWW20654.1"
                     /translation="MSMQRMACVAFTLLGVTVLDGAAFAGDRDGVSEELEGIAATWPD
                     ALFSIDVHGTDDGNAALNQRLEIVYEAASPGYLAYLRVSSHGDVTLYRDLAKAGRTSG
                     TQPYLIKPPLGSEQLIVLFANKPWDALFAKGATTRELGGERTDAATLVKQLAQLHDSD
                     LLFAARRYQLQIAASPGGTEYTTRAIVRRVKEGGGGSGTRIPSRIQFEFNSDQLTPQG
                     KLDLDTFGEALITDLRNSGVALEGHTDAIGADDYNMDLSERRAAAAKQYLIDSFGIAA
                     SRLSTVGMGKDNPIASNGDEAGRSKNRRVDFIFSAAH"
     gene            complement(9836..>10099)
                     /locus_tag="VNZ06_07610"
     CDS             complement(9836..>10099)
                     /locus_tag="VNZ06_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20655.1"
                     /translation="HVQARSNHGIVLSTSGGPIRLMIPSDTHGTLDAETSGGHVRSQI
                     PLSVTELSESNHLRGQINGGGDRISLHTSGGGIEIAALAPAAH"
BASE COUNT         1830 a         3027 c         3165 g         2077 t
ORIGIN      
        1 cccgaagcgg tggccgccgg cgagcgctac tttccggacg agatgcctga tcgacgctat
       61 tatcatccga ccccgcgtgg gttggagcta cgcattggcg agacgctgca gcgtctgcgc
      121 aaaacgatcc ccgagaaagg cgatacatga tcgatccgaa actgttgcgg tcggcgcctg
      181 aagagatcgc gcgccagctc gcgcgccgcg gctatcaact cgacgtcaca gcatggcaga
      241 gactggagga gcgccgccga cacgggcaga tcgagaccga ccgactgcgc gccgcacgca
      301 acgcgcacgc gaagagcgtg ggtcaggcgc gcgtccgcgg cgaggatatc gcgcctctga
      361 tgcagcaggg tgagcaactt gccaaggagc tcgctggcgc ggagcaggaa ttcaatggcg
      421 tgcaggctga gctggatcgc tggcagctcg acatcccgaa cctgcctcat gaatcggtgc
      481 ctgcgggcac ggatgagagc gccaatgtcg aagtagcccg ctcaggtgag ccgcgtcagt
      541 tcagcttcaa gccgcgcgat cacgtcgagc tcggcgagcg gctcggcgcg ctggacttcg
      601 aggccgccag ccgcatatcc ggaacgcgtt tcgtcgtgat tcgcggcgag gtggcacgac
      661 tgcagcgcgc gctcgcacag tacatgctgg acctgcacac gcgcgcacac ggttacaccg
      721 agatctacgc accctacctg gtgctaccgc atgcattggt gggcaccgga cagttgccaa
      781 aattcgaaca ggatctgttc gcggtgcgcg gtgagcctgg gttctatctg attccgacct
      841 cggaagtgtc gatgaccaac ctggtgcgcg agcagatcat cgctgccgag gcgctaccgc
      901 tgaagtttac agcgcacacg ccctgctttc gctccgaggc gggagcggcg ggccgagata
      961 tgcgcggtat gatccgtcag caccagttcg aaaaagtgga actggtgcag atcgtgcgac
     1021 ccgctgattc ctacgcagcg ctggagaacc tgcgctcgaa tgcggaaaca gtgctccaga
     1081 aactggagtt gccgtatcgc gtcgtagcgt tgtgtggcgg tgacatgggc ttcaccgctg
     1141 ccaagaccta cgatctggaa gtgtggttgc catcacagga gaagtatcgg gaaatctcct
     1201 cctgcagcaa ctgtgaagcc tttcaggctc gtcgcatgca ggcgcgctgg cgcaatcccg
     1261 aaagcggcaa gcccgagttg ctgcacacac tgaacggatc aggtgtcgcg atcggccgcg
     1321 cgttggtagc ggtcctggaa aactaccagc aggcagatgg cagcgtcgaa gtgccctcgg
     1381 tcctgcgacc ttacatgggt ggtgttgcgc tcatcggcaa gggcatccgc agctagccgc
     1441 gcgtcccacg tcatggagcc ttcagcgccc ggaaaaacac ggcgattcgc tctgccgctg
     1501 cagatcgtgc tggcggtgat cctgggcgtc gtggtggggc tgctgtggcc caagaccggc
     1561 gctgcgatgc agccattggg aaccgcgttc atcaacgcgg tcaagatgat cgtgataccg
     1621 atcgtctttt gcgcggtcac gctcggtgcg atgaagatgg gccgcgatgc tccacgcctg
     1681 ggacgcatgg cgttgatcgc gttcgtctgg ttctatttct ccagcgcggt tgcgatcgcc
     1741 atcggcctga ttctggacac actgttccac ccgggtcttg gtattcacct ggatgtcacg
     1801 ggtcctgcac cctcgcagct cacaaacggg attgactggg tcaaatttct gctggagctg
     1861 attccttcga atatcgtgtc ggcgatggcc aatcagaagg tactgccaac gttgttgttc
     1921 gctgtgctgt tcggtcttgg cctgtccgcc gtgggtgagc cgcgcgccaa gccgatggcg
     1981 gatgttctgg agagtgtgct ggccgtgatg tttcgggtga ccaactggat cgtatcgctg
     2041 gcacccatcg ccgtattcgg cgtggtcgca tggctgtttg ccagccaggg ttcggcgacg
     2101 gtgtgggcgc tggccagact gatcggcgtt gcctatctgg gctacaccat ttttgggctg
     2161 ctgtgcgtgc tgggttttgc ctggctgggc gaaccgccga tcgctctgac acgggccatg
     2221 tcgggtccga cgctgctggg gttcgtgacc cgatcatcag aggcttcgtt gcctttgcat
     2281 atggaaaagc tcgagcaatg gggcattccc aaccgcatcg tgtcggtgat attgccactc
     2341 ggctatagct tcaatctggc cggagccgca ctgtacatcg gcctggcgac gacattcatc
     2401 gctgaagcgt acggactgcg cctggcattg ccgcagttgc tgaccgtcct cgccaccgcg
     2461 ctcatcgcca gcaaaggcgt ggccaatgtg ccgtccgcat cgttgatcgc aatctccacc
     2521 gtactactgg cgctcggcat accggccgac gcgatcgcga tcattgccgg cgtggatgtt
     2581 tttctggata tgggacgcac ggcaatcaat gtctttggaa atacgctatc agtgctggtg
     2641 gcccgacggt acggcgaagg agagggcact ggtggttgac gcatcgtata cgacccgaat
     2701 gcagttcacg gcgcgccgcg tgctgtcgct cactgatctc acgagcctga acgacaacga
     2761 cgatgagcgc agcatccaga agctgctgaa gctggcgtcc accgatgctg ggaaagtggc
     2821 ggccgtatgc acctgggcgc gattcattgc ggtcgcgaaa ccgatactcg cgcgcactgg
     2881 aatcccgttg gcggtggtcg cgaactttcc cgctggcgcc gccgacccgg tggcagccgc
     2941 agcccagacg accgcggcgg tgaccgcagg tgccaatgaa atcgatgtgg tgtttccgta
     3001 tcgagcgctg atgagcggcg atgagcaatc cggcctcaat ctcgttgagg cctgccgcgc
     3061 cgcctgcggc gaacagacgc tgctgaaggt cattctggag accgggcagc tcggatcgga
     3121 ccgcaacatc agtacggcgg cgcgaattgc ctgtgacggt ggcgcagatt ttctgaagac
     3181 ctctaccggg aagacgcagc ctggcgctac gatcgaagca gtcggcgtgc tgctcgacgt
     3241 gattgcgatg tacgccaagc gtggcaggaa ggtcggactg aaggtgtcgg gcggggttcg
     3301 taccgtcgag caggccaagc agtacctgga tctctacgag gactacctgg gtgtcggggc
     3361 ggcaacgccg gcgaccttca gaatcggtgc ctcatcgctg gtgagcgata ttctgacggt
     3421 gctgcgtccg aatgaaaggg acgttcagca gtcttagctg tcgacagcgc gttcgggccg
     3481 tcgtcgcccc tgaactgcag tccgaggccg agtcaggcag aatacgcacc ccatgctgcg
     3541 gagaggtggc agagcggttg aatgcaccgg tcttgaaaac cggcgttcct ttacgggaac
     3601 cgtgagttcg aatctcaccc tctccgccaa taaaaacaaa tacttgaggc ctgcgcccta
     3661 cccgcgcggt atgcattcga atacgaatgg cccgggttcg ctgttggacc aattcatggg
     3721 tgtgattgga atcactgcag cctcctcgcg ttgctccgag cagaagctgg tcgctacttt
     3781 cacggcgttg tcatctgcgc cgcaccatac gcagctttta gccgggaatt ggacctcata
     3841 cgccatctgg cccatcggaa caactttgta gctggcgcag gcagacgcca gaacggcaat
     3901 gcctgcaatc aacatgtgct tcattttgag cttccctgtt ttcatacgcg caattgtgca
     3961 gcgagggctg ccagcgctct gtcaaccggc ggactcaccg actggctcgc aacttccggc
     4021 ggtgccgcat gagcgccgtg gcgttcgact ggaaacagcg cgactaccgc ccgatttacg
     4081 cccagcgcgc ggccacgctg gctcgcgatg gcgctcgccg catcgctctg cctcttcaac
     4141 cgcggaatgg cgatcggcgt cggctcggcg atggaggata agctcgatcg gaccggcgac
     4201 ccggattgtc tcttcaggaa ggcgcgcacc ttcctgaaga atctgcccgc cgaattccgt
     4261 ggcggctggg atgagacgcg ccacaccgca cacatgcggt tctaattgaa gcccacaagc
     4321 gcgggccggc ggcgccatgt cgcattgatg tttcaggagc gccgaaaacg ctggaatgac
     4381 tgggagagcc gcttttctag gtctctgaca actttgtgct tacgggtgcc tcaggggcgg
     4441 gtaaacgtgg gtcaggagtc ttgaaaaccg gcaacggggc aacccgttcg tgagctcgaa
     4501 tctcaacctc tccgccaaat cgttgggtga caccgcgaga tcgacgactt catgaccgtc
     4561 ccgcgctcgc gcccagggtg acgagcagct catccagttg ctcgaacgac tcgcccgtcc
     4621 cacccgtact cccagaggca atggcagcgt cgagagcttc cttcgaggga taaaggtcgt
     4681 gcataacgac cagcgtctgg tcacctcttt cttgaaaggt caccgtggtg acggccccac
     4741 cttcaccacc ttcgtcattc gtccacacga cgcgggagtg cggcgtcact tcgatgtacc
     4801 tgccgaagaa cgccataggc tgctctgagg cagcgtgacc gaacacgaaa cggtacgtgc
     4861 cccccgtacg aacatcggcc tcgcaggaaa gaaaggacac tccaaacgac ttcggtgccc
     4921 accaccgctt gagcagctcg ggcttggtcc aggcctcgaa caccatgcgc gccgggccgt
     4981 tgaaggttcg cgtaacgacc agctcgcgct cggacttccg ctccaccgtc gtgcggttct
     5041 tcatgttggc tcactctctc ttcttgcgtc catcgacctt ctccttccgt ttcagctcct
     5101 cgacaacctt gtccagctcg tcgaagcgtg cgtcccagag ctggcggtac ctctcgatcc
     5161 atgccgcctc ttcctccagt cggcgcaggc cgagcttgca ggtccgcacg cgcccgacct
     5221 tcttcgtggt gacgagcccc gcctgctcca agacgccgac atgcttcttc atgccggtga
     5281 gagtcatgtg gaacttctcg gcgagctccg tgattgaagc gtctgcgcgc ccgagctgct
     5341 ccagaacgcc gcgtcgggtg gcgtccgaga gcgcagcgaa cgaggcatcg aaacaggccc
     5401 ggatatactg aaccatatgg ttcagtattt aacacacgca ttcgcagcat gcaaggggtg
     5461 gccgaacgcg cgctgcgttg ctggccgcct tcaccgccaa atcgagctca tttcgttgta
     5521 gattcgatcg actcggcggg tgggccagta cccggcagcc cttcacgggc tcatgagttc
     5581 gacaagatcg cgcgagcgat tttggacgca cgccgtgcgc ccggaggcta tgccgctgtc
     5641 gtgttagtca atatgacagt agggatgagt ggcagctttg ctgactgggt atgcatagtg
     5701 cgcagcgcct ggcccggaaa gccgccgtgt cactggagaa cgggtaggcc aagtctgggc
     5761 tcgcagctct cagctgcgag aatccacctg atttcgtcca cttgcggctc gctcagactt
     5821 gatttcctcg ctgagccttc gcatgtcatc gagtgtcctg cgccgggcgg tgtcggtgac
     5881 atggattgga cgacggttgt aagggtcatc ggaagagaac aggtcgacga ccttctcagt
     5941 cttgccgtgc gaatgcgatc cctgcgacat ctgctctctc cattgcctcg gtgcaagtaa
     6001 gtaccacagg aatgcaccct gggttgtgcg ggcaatgtag cgtgcggcct ctgtttcgcg
     6061 cgatatgtat tgatagcgcg acaaccgggc gctttgcgcc agaaagcgac gcaaaatgat
     6121 gcgtcgttga gattatgttg tgcctgttag gttcaatcga ccagacagtc gccggcgaaa
     6181 ctatctgggc agcgctccac gtgtgcccgg ccggcggata ggcccggctc ggatacagca
     6241 gccatgctcc gacactcatc gatgagcgag tttctgctcc acagtcgaga acaaatcact
     6301 gaagaacgct gcattggccg ggccttcctt gctgatgact tcagagttct gtcccccgga
     6361 gactgttgcc atcaaggacg ccatctgatc gggcgagcag tcgccagtac tgcaggccga
     6421 ggagcccagc actcctgcga gcgcattcat ctgtcttcgc gagatgccgt aaagcgaggt
     6481 caagctcgag tgagcttcag attcgctggc ggaggccgcc aatcgtgcgc cagcgtcgtc
     6541 cgaattgacg acgaccgaga cattcacata gtttgccgcc cagccgtgcc cttccttggc
     6601 ggtggttcgt cgattcgcca ggatgaggcc gaggttcctg tcgagcttcg tgatcgtgaa
     6661 gcccttctgg gccaatccgt cgtttgccgc ggtcaataga ttttcaaggg tcgtggcaaa
     6721 gtgttgctga tggctggcct ttggtgcgtc gccgccgcgc agattggtat cgatcccggc
     6781 gagcagatcg cgatagaaac cctgatcgtt gaccgggcct tctttgacga ccacgttctc
     6841 gtaatcgcgc ggctgatagc cgaaggtggc ggccgcgatc cggctgccgg gtttgcgatg
     6901 gagcaggcgc tgctcgctcg ccgtgatctg cacgcgggcg atcgttggcg aagaggtcgg
     6961 cgatgctgag gctgtgagcg cggtatccgt cgagtagctc tcattcttgg gatcctgcag
     7021 ggcgtacacg gcgttaatga ggccgagttc ggcactcacc gactgaatga tgaatccgcg
     7081 ctgcacgagc gtctgaatga gggccatacg cgtttgcggc accgaggcgc tataggtctg
     7141 cccgtttccc gaggcgcctg ggtccgtgtc cgcgggcgta ctgagcggcg cgggcgtttg
     7201 tagcgtgacg ggtacggcac gggcagcctc atcgttcgtg gtcgcgggtg cgtcgtcttt
     7261 cgtatcgcct ggcgcagcgg tcgcatcacc cgatgcggcg cctgcgggct gcgctgccgg
     7321 tgcgacaccg gcagaggcct cgcttcgaag attgcggtcg accgcgtcaa agaactcgcc
     7381 atagaacgcg gagtcggtga cgttgacctc cttcttgacc acgtccttgt actctttgga
     7441 cgtcggtatc ggaatgattg ccagcgtccc ccaatgatga aattcgtcgt gcagaacggt
     7501 gcgttcgatg gcagtgagcg taaccaccga gtgatccgtc gtgggaatgg gcttgacgtt
     7561 gacggtcgca gacagaacat cggtcagttt cttgtcgtgc gggtcatcag tgctgcgcat
     7621 cgcatcgata tagccggcct cgatactgct gttagcgatg cgaaatccgc gctgcatcag
     7681 cgtcatcgtg acggcgcgca acgtctgttc cggggttgcc gagaagcagc gtccgacatc
     7741 attcagcacg gtcttgtcac aattcatcga tgtcaccgtg gcggcgagtc gggcttccat
     7801 ctcgcggtta tgcgcttcca cttcgcggtt gtgcgcttca agctcggcct tctcctggcg
     7861 ggccttctcc agttgctcat catttgcttt ctgcatcttt tccagctcgg cgcgcgccac
     7921 cttgtcgatg cagccggcga aacaaaactc accggtgaaa ttggtataga gcgccggtgt
     7981 ctgcgtgtgg ccaacggccg cggcggagtc gccctgcacg ccctcgctga cacgcttgaa
     8041 catttcgtcc accgtgaggc cggggatctt gatgtttgcc agcaggttct tggtgagcgt
     8101 tccgttgcgt ccttcgccgt cgtcggagac gtcattggcg gctgtggcat acagcaccat
     8161 cgttccgcgc gggatggcgg tgattggcgc aagtccgctg ctcataccgc gtggattgtc
     8221 tgcccacgga ttgctgcggc aggcgtcgag tatcacgatg ttcagatagt gcttggcctg
     8281 caggagctgc gtcatgatgt agttgagact aacggtttcg cgcaccacat cggtttcagc
     8341 gcgcagcttc gccgaggtcg gcaccaggta gttctcgccc ttgagctgca ctgcatgtcc
     8401 ggcgtagtag aacagcactt ccgacttcgg cttgagtgcg gcggcaaagt cctgaatgcg
     8461 cgctttgaac tcgcgagcgt ccttgacatc cacatagcag gagacggtgt agccgagctc
     8521 ggtcagcgca tcgcacatgt catgcgcgtc gtaggctgag ttcttgagcg cagtgacggc
     8581 actataggcg gcgttaccga tgacgagtgc tgcacgcggc cgcgcggctc cggcgtccgc
     8641 ggcacttgcc cggggagtcg gccaggcgca aagcacgagg gtcagcagcg caatgccata
     8701 cgagcgcgtg gtcagcatag atagccccct gagcttgcgg ctcagttctt agtgtgctgc
     8761 ggagaaaatg aagtcgaccc gacggttctt gctccggcct gcctcgtcgc cgttcgaggc
     8821 gatgggattg tccttgccca tgccgacagt actgagcctc gaggctgcga taccgaagct
     8881 gtcgatcagg tactgcttcg cggctgccgc tcgtcgctct gacaggtcca tgttgtagtc
     8941 atcagctccg atcgcatcgg tgtgcccttc cagggctacg ccggagttgc gcagatcagt
     9001 gatcagcgcc tcgccgaaag tatccaggtc aagcttgccc tggggggtca gctggtccga
     9061 attgaactcg aactgaatgc ggctcggaat tcgggtgccc gacccaccgc cgccttcctt
     9121 cacgcggcgc acgatggcgc gcgtcgtgta ctcggtgccg ccggggctag ccgcgatctg
     9181 caactgatag cgtcgtgcgg caaacagcag gtcgctgtcg tgcagctgcg ccaactgctt
     9241 gaccagtgtg gccgcgtcag tgcgctcgcc gcccagttcg cgggtcgtcg cgcccttggc
     9301 gaacaacgca tcccagggct tgttggcgaa caacacgatg agctgttcgc tacccagcgg
     9361 cggcttgatc agatagggct gggtaccgct ggtccttccg gccttggcca gatcgcgata
     9421 cagcgtgacg tcgccgtgac tggacacccg cagatacgcc agatagcccg gagaggccgc
     9481 ctcatagaca atctccaggc gttgattcaa tgcggcattg ccgtcgtccg taccgtgaac
     9541 gtcgatggaa aagagggcgt cgggccaggt ggcagcaatg ccttccagtt cctctgagac
     9601 gccatcccga tcacctgcaa acgcggcgcc atccagcact gttacgccta acagagtgaa
     9661 cgcgacacat gccatccttt gcatgctcat gggcccctcc caatggggta tctaatgtgc
     9721 acccgtcggt gctatggggg caatactgcc tgggagggga gtgtgaaccg gtcagagcca
     9781 ggcttctgtc cagtccctga cagaagcccg cttttgagtg acttgcgacg ggttctcagt
     9841 gagcggccgg tgccagcgct gcgatttcga tgccaccgcc tgaggtgtgc aacgaaatac
     9901 gatcgccgcc gccgttgatt tgtccgcgca ggtgattgga ttccgacagc tcggtcacac
     9961 tgagcggaat ctgactacga acgtgaccgc cgctggtctc ggcatcgagc gtaccgtgag
    10021 tgtcgctcgg tatcatcaga cggatcggac cgccggaagt ggacagcacg atgccatggt
    10081 tggaacgcgc ctggacatg
//
LOCUS       DATMRM010000259         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3861592, whole genome shotgun sequence.
ACCESSION   DATMRM010000259 DATMRM010000000
VERSION     DATMRM010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3861592"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VNZ06_07615"
     CDS             complement(<1..193)
                     /locus_tag="VNZ06_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWW20656.1"
                     /translation="MTANSHRDAYDVIVVGSGAAGGQSAYALCMEGAKVLMLEAGRRY
                     SPETETPMFQTPDLAPLRGV"
     gene            complement(222..1433)
                     /locus_tag="VNZ06_07620"
     CDS             complement(222..1433)
                     /locus_tag="VNZ06_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HWW20657.1"
                     /translation="MRLSILTAALQELTPRDRRDPDPDLAIEEWLEFARELRCPNIEL
                     SAALHPTEGDVPAAALLDPVANTLNLRQSFSAARASRVRRAMQATGVGLSDLAYFDNM
                     LAADASVRAKKHELMLRVFDAAVLLGTDAVTGFVGRNPLLGMDANLVMFEEVFVPLLE
                     QAKARGLTFRVEQCPMPGWNITDTWHNNIAYAPGPWIALHRICARHGVGDQFRIHYDP
                     SHAILMGQDSRSVFQYLKDAGYNFLISGFHVKGQVIDSRGISAWGYAGQTMQRGDWHG
                     DRPSADPVEQQNAWKKQTVFCEHELPGTARHDPLAYLQNRSVDWLDHQLAARELLSIE
                     PEKTFLVVEHEYPKARVQDKARLAPILAASLAFTRAIDEAAAAMYALQHEVLAAQGIS
                     VQGIGREAYRS"
     gene            complement(1465..>2067)
                     /locus_tag="VNZ06_07625"
     CDS             complement(1465..>2067)
                     /locus_tag="VNZ06_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW20658.1"
                     /translation="VLLRQRNFVVFFVASILICIPLAFYYQNANQFLTESQVANATGK
                     QTIGQMSEVLFMFLIPVFLRTFGMKTTLLLGMLAWVCRYVLFAFGNAHELGFMLIVGI
                     ALHGACYDFFFVSGQIYTDSKAGPRHKGAAQGLITLATYGVGMLVGFWAAGLTDDLYS
                     LGGAHDWQSIWLYPAGFAAMVSVLFASTFRNEKVAYANPH"
BASE COUNT          430 a          631 c          630 g          376 t
ORIGIN      
        1 ccacacccct cagcggcgcc aggtccgggg tctggaacat gggtgtctcg gtttcgggcg
       61 aatatcggcg cccggcctcg agcatgagta ccttcgcacc ctccatacac agcgcatagg
      121 ctgactgccc gccggcggcc ccggagccga ccacgatgac atcgtaggcg tccctgtggg
      181 aattcgccgt cacgaagggc atgccgatac cactcgagct ttcaggatcg ataggcttcg
      241 cggccgatgc cctgcacgga tatcccctgg gcggcgagca cctcatgctg gagggcgtac
      301 atggccgcgg cggcctcgtc aatcgcgcgc gtaaaggcga gggaggcagc cagaatgggc
      361 gccagccgcg ccttgtcctg cacgcgagcc ttgggatatt catgttccac gaccaggaag
      421 gtcttctccg gctcaatgct gagcagctcg cgtgcggcga gctgatggtc caaccagtcc
      481 accgatcgat tttgcagata cgcgagcggg tcatggcgcg cggtgcccgg cagctcgtgt
      541 tcgcaaaaga cagtctgctt tttccaggca ttctgctgct caactggatc ggcgctcggc
      601 cggtccccgt gccagtcccc gcgttgcatc gtctggccag cgtagcccca tgctgagata
      661 ccccgcgaat ctatgacctg gcctttgaca tgaaagccgc tgatcagaaa gttgtacccc
      721 gcgtccttca gatactgaaa cacggagcgc gagtcctgcc ccatcaggat tgcatgcgag
      781 gggtcatagt ggatgcggaa ttgatcgccg acgccgtgcc gtgcacagat ccggtgcaag
      841 gcaatccacg gacccggggc ataggcaatg ttgttatgcc acgtatcggt gatattccac
      901 cctggcatgg gacactgctc gacccgaaac gtcagcccgc gggctttggc ctgctcaagg
      961 agcggcacga acacctcttc aaacatcacc agattggcgt ccattcccag caggggattt
     1021 cgccctacga agcccgtgac ggcatccgtc ccgagcagca ctgcggcgtc gaagacgcgc
     1081 agcatcagct cgtgcttctt tgcgcgcacg gatgcatccg cggcgagcat gttgtcgaag
     1141 taggcgaggt ccgacaggcc gacgcctgtg gcttgcatgg cccggcggac gcgtgatgcg
     1201 cgcgccgcac tgaaggactg gcgcagattc aaggtgttgg ctaccggatc aagaagtgcc
     1261 gcggccggca catcgccttc cgtcgggtgc agggccgcgg agagctcgat attggggcag
     1321 cgcagctccc gggcaaactc caaccactcc tcgatggcca gatccgggtc ggggtctcgc
     1381 cggtcacggg gggtcaattc ctgtaacgct gcggtaagta tgctgagccg cattggtctc
     1441 ggtctgaatg cggcctccga gggattagtg cggattggca tacgcgacct tttcattgcg
     1501 aaatgtcgac gcgaacagca cagacaccat ggcggcgaag ccggctggat atagccagat
     1561 agactgccag tcgtgcgcgc cgccgaggga gtacaggtcg tcagtgagcc cggcagccca
     1621 gaatccgacc agcatgccga caccataggt ggccagggtt atcaggcctt gggctgcgcc
     1681 cttgtgccgg gggcccgcct tggagtcggt gtaaatctgg ccggagacaa aaaagaagtc
     1741 atagcatgcg ccatggagcg cgataccgac gatcagcatg aatccaagct catgcgcatt
     1801 gccgaaagca aacaatacat accgacacac ccaggccagc atgcccagca gtagcgtggt
     1861 tttcatgccg aaggtcctga ggaataccgg aatcaggaac atgaacaaca cttccgacat
     1921 ctgaccgatg gtctgcttgc ccgtcgcgtt ggcaacctga ctctccgtta gaaactgatt
     1981 ggcattctgg taatagaacg ccaggggaat acagatcagg atagacgcta caaaaaacac
     2041 gacaaagttc ctttgccgca acaagac
//
LOCUS       DATMRM010000260         5284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4174838, whole genome shotgun sequence.
ACCESSION   DATMRM010000260 DATMRM010000000
VERSION     DATMRM010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4174838"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VNZ06_07630"
     CDS             complement(<1..250)
                     /locus_tag="VNZ06_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20659.1"
                     /translation="MAAEHVESPNEYVTHHLTHLKVGSGFWTWHVDTLVMSALLAVAV
                     SVLLWAAARRVKQGAAPTGLAAFIEWVYEWVDGEVRDSY"
     gene            complement(266..709)
                     /locus_tag="VNZ06_07635"
     CDS             complement(266..709)
                     /locus_tag="VNZ06_07635"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HWW20660.1"
                     /translation="MSEWPLTGSLVPTSNSDSGNRPVAGRILRVQIGVGAAVALVSLA
                     FWGRTALISALFGAATGVIANAYMMFKALRPARTARAALAAVYMGQVVKFALTVAAFM
                     WAARLPHVSWLALMSAYVGTLAVSWWVPMAATPRKIGSEGASLQR"
     gene            complement(829..1746)
                     /locus_tag="VNZ06_07640"
     CDS             complement(829..1746)
                     /locus_tag="VNZ06_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009402535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HWW20661.1"
                     /translation="MTQKKPTLGRGLADLLGQVRPATGANPGAQDGAARKPADDELTR
                     LPLDLLQRGRYQPRMDMRPETLTSLAESIKAQGVVQPIVVRPIGEPDERGIQRYEIIA
                     GERRWRAAQQAGLKEIPAVIRRVPDEAAIAMALIENIQREDLNPLEEARALDRLITEF
                     GLTHQQAADAVGRSRAAVSNLLRLLELPPEVCERLEKRELEMGHARALLGLPHRRQQV
                     EIASFVVKKSLSVRDTEALVRRTVEPAKPAADSGAVAIDPNIQRLEDELADKLGARVN
                     IEHARAGHGRVVIRYNSLDELDGILGHLR"
     gene            complement(1759..2574)
                     /locus_tag="VNZ06_07645"
     CDS             complement(1759..2574)
                     /locus_tag="VNZ06_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014705464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWW20662.1"
                     /translation="MKRVIAVANQKGGVGKTTTAVNLAASFAATRRRVLLIDMDPQGN
                     ATTGSGIDKRSIARGSCEVLLGECTAGAAVLQAGSFAIIPTNGDLTAAEVRLLMMQSG
                     RERRLREGLDPIRGDYDLVVIDCPPALNMLTVNSLVAADSVLIPMQCEYYALEGLSAL
                     VQTIEQIRDAANGDLEIEGILRTMFDPRNNLATEVSAQLMMHFGEKVFRTVVPRNVRL
                     AEAPSFGKPALQHDKDSRGALAYLALAGEMLRRDDAVGALNTVGSVGSAGAPP"
     gene            2628..5249
                     /locus_tag="VNZ06_07650"
     CDS             2628..5249
                     /locus_tag="VNZ06_07650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW20663.1"
                     /translation="MKLISGIVVAAIFAVVTFGLWAYLNSPVSEPPWPAHGIQGVAFS
                     PYAADQNPFGSKDVPPIEQMDSDLKLLENKTFAVRTYSVQGSLGKIPELAAKHNISVA
                     LGVQLLKEEPENETELRIGIALAHEHRNIVRVIVGNEVLLTGALSEDKLAAYLDRARR
                     AIRQPVGYADTWATWLRHPEITKHVDFIAVHLFPYWEGVDVESAVDFCFRELKAVQDA
                     YPKKPVIIGEIGWPSEGRIRGGAVASVSNEALFLRQFLARAEKEGERYYLMEAFDQPW
                     KARDEGAVGAYWGIYDVNREPKFEFVQPIVRIPEWHLLAAISVALALVMLGVFYMNSG
                     SLRTRGRTLLAVVVYAAATITVWVIYDYTQQYLTPTSVVVGSLLILGMLGVVAVLFAE
                     AHEWAEAHWTGAHVRMLDPTRGGNPSLRKVSVHVPAYNEPPDMLIETLDALARMNYPN
                     FEVVVVDNNTRDEAVWRPVEAHCQKLGERFRFFHVAPLAGFKAGALNYALARTAPDAE
                     VVAVIDSDYKVEPNWLSDLLPAFDSPRMAIVQAPQDYRDDNDNVFKAMCYAEYRGFFY
                     IGMITRNERNAIIQHGTMTLVRRYVLSAVGGWAEWCITEDAELGLRVFEAGYEAIYIA
                     QSYGRGLMPDTFVDYKRQRFRWAYGAMQILRHHASALLQPSASALTAGQRYHFIAGWL
                     PWIADGFNLLFNIAAVGWSIAMIVAHPKIDPPLMVFSVLPLSLFMFKLVKLVHLYTNR
                     VGMNGRQTAAAAISGLALAHTIGRAVLKGLISGNEPFFRTPKRVAPHRFFAALGAARE
                     ETLLMLALWASALGIHLLPKAMASPDLTVWLAALLIQSIPYTAALLVSLASAFHLPAS
                     IIGHRRQGSVNTPAAQE"
BASE COUNT          953 a         1706 c         1637 g          988 t
ORIGIN      
        1 gataggagtc gcgcacctcg ccgtcgaccc actcgtacac ccactcgatg aatgccgcca
       61 ggccagtcgg cgcggcgccc tgcttcacgc gccgggccgc ggcccacagc aacacgctga
      121 ccgcgaccgc cagcagcgcc gacatcacaa gcgtgtcgac atgccaggtc cagaatcccg
      181 aacccacttt caaatgggtc aagtgatggg tgacgtattc gttcggtgac tcgacgtgct
      241 cagcagccat ctgatgctac ggtccttacc tttgaaggct tgcgccttca ctccctatct
      301 tccgcggcgt cgccgccatc ggcacccacc acgacacagc cagcgtcccg acataggcgc
      361 tcatgagcgc cagccaggac acgtgcggta gacgtgccgc ccacatgaac gcggcgaccg
      421 tcaacgcgaa cttgacgacc tgccccatgt acacagctgc taaggccgcg cgcgcagtcc
      481 gtgccggacg caatgcctta aacatcatgt acgcgttggc gatcacaccg gttgcagcac
      541 cgaaaagtgc cgaaatcagt gcagtgcggc cccagaacgc gaggcttacc agcgccaccg
      601 cggcgcccac accgatctgc accctcagga tgcggccggc gaccggtcgg ttgcccgaat
      661 cgctattgct cgtcggcacc aggcttccgg ttagaggcca ctcactcata cgctctcgac
      721 gccgagtgga ttggatttta cttatcgggg aagcctgcga aagcacccgc gacaagcggg
      781 cgcgattata gagatgaagg caggtagctt caagaaatct aaagagaatc agcgcaaatg
      841 tccgaggatt ccatccagct catccaggct gttgtagcgg atcacgacgc ggccgtgtcc
      901 ggcgcgcgca tgctcaatgt tgacccgtgc gccgagcttg tcggccagct catcctcaag
      961 ccgctgaata ttggggtcga tggcgaccgc accgctgtcc gccgctggct tcgctggttc
     1021 caccgtgcga cgcaccaaag cttccgtatc acgcaccgac aggcttttct tgaccacgaa
     1081 cgacgcaatt tcgacctgct ggcgtcgatg cggcagaccg agcaatgcgc gcgcgtggcc
     1141 catctcgagc tcacgcttct ccaggcgttc gcacacctcg ggcggaagct ccagcagacg
     1201 cagcagattg ctgacggccg cccgagagcg tcctacggcg tccgcggcct gctggtgtgt
     1261 cagtccgaat tcggtgatca atcgatccag cgcccgagcc tcctccagtg ggttgaggtc
     1321 ctcacgctgg atgttctcga tcagcgccat cgcgatcgcg gcctcgtcgg gtacccggcg
     1381 gatgaccgcc ggaatctcct tcaggccggc ctgctgggcc gcacgccaac gacgctcgcc
     1441 ggcgatgatt tcgtagcgct ggatgccccg ctcgtccggc tcgccgatcg ggcgcacgac
     1501 gatcggctgt acgacgccct gggctttgat cgattctgcc agcgacgtca gcgtttccgg
     1561 ccgcatatcc atgcggggct gatagcgccc tcgctgcagc agatccagcg gcaggcgggt
     1621 cagttcgtcg tccgccggct tgcgcgcagc cccgtcctgc gcaccggggt tggcccccgt
     1681 tgccggcctc acctggccga gcagatcagc gagtccgcgt ccgagcgtcg gcttcttctg
     1741 tgtcatggtt ggataatatc aaggcggcgc tccggcgctg ccgacgctgc ccaccgtatt
     1801 cagcgccccc accgcatcgt cacgccgtaa catttcgccc gcgagcgcca aataggccag
     1861 cgcaccacgc gaatccttgt cgtgctggag cgccggtttg ccaaaactcg gcgcctcggc
     1921 cagtcgcacg ttgcgcggca cgaccgtgcg aaacaccttc tcgccgaagt gcatcatcag
     1981 ctgcgcgctg acttcggtgg ccagattgtt acgcggatcg aacattgtgc gcaggatgcc
     2041 ttcgatctcg agatccccgt tggcggcatc gcggatctgc tcgatggtct gcaccagcgc
     2101 cgacaggccc tcgagggcgt agtactcgca ctgcatcgga atcagcacgc tgtcggccgc
     2161 caccagtgag ttgaccgtca gcatattgag tgcaggcggg cagtcgatga ccacgagatc
     2221 gtaatcgcca cggattggat caagcccctc gcgcaagcgc cgctcgcgcc cggattgcat
     2281 catcagcagg cggacctcgg cggccgtcag gtcgccattg gtcgggatga tggcaaagct
     2341 acccgcctgc agcaccgcgg cgccggcggt acactccccc agcagcacct cacaggagcc
     2401 ccgcgcgatc gagcgcttgt cgataccgct gccggtggtg gcattgccct gcggatccat
     2461 atcgatcagg agcacacgcc gacgtgtcgc tgcgaacgac gcagcgagat tgaccgcggt
     2521 agtcgttttg ccgacacccc ccttctggtt cgcgactgcg atcacccgct tcatgacatc
     2581 tttagtctag ccgttgacgg gtcaggacta caggtaaact caccggcatg aagttgatct
     2641 ccggaatcgt tgtggccgca attttcgcgg tcgtgacttt cggactatgg gcatacctca
     2701 atagcccggt aagcgagccg ccctggccgg cgcacggcat ccagggcgtc gcgttctcac
     2761 cctatgccgc tgaccagaac ccgttcggca gcaaggacgt accgccgatc gaacagatgg
     2821 actccgatct gaagctgctc gagaacaaga cgttcgcggt gcgcacgtac tccgtgcagg
     2881 gctcgctcgg caagattccc gagctggccg cgaagcacaa catcagcgtc gcgctgggtg
     2941 tgcagctcct caaagaggag cccgagaacg agactgagct tcggatcggc attgcgctcg
     3001 cgcatgaaca ccgcaacatc gtgcgcgtga ttgtcggcaa cgaagtgctg ctgaccggcg
     3061 cgctgagcga ggacaagctc gcggcctatc tcgatcgtgc gcggcgagcc atccggcagc
     3121 cggttggcta cgccgatacc tgggccacgt ggctgcggca tccggaaatc accaagcatg
     3181 ttgacttcat cgccgtgcat ttgtttcctt attgggaagg cgtcgacgtc gagtcggcgg
     3241 tggatttctg tttccgcgag ctcaaagcag tacaggacgc ctaccccaag aagccggtga
     3301 tcatcggcga aatcggctgg ccgtccgagg gccgcatccg cggcggggcc gtggcctcgg
     3361 tttcgaacga agcgctgttc ctgcgccagt tcctggcgcg tgcggaaaaa gagggcgagc
     3421 gctactacct catggaggcg ttcgatcagc cctggaaggc gcgcgatgag ggcgcggtgg
     3481 gagcttactg gggcatctac gacgtgaacc gggagccaaa attcgagttc gtgcagccga
     3541 tcgtgcgcat cccggagtgg catctgctgg cggccatctc ggtggcgctg gcgctggtga
     3601 tgctgggcgt cttttatatg aacagcggtt cgctgcggac ccgggggcga actctgctgg
     3661 cggtagtggt ctacgccgcg gcgacgatca cggtgtgggt catctacgac tacacacagc
     3721 agtatctgac cccgacctcg gtggtggtgg gttcactgct gatccttggc atgctcggcg
     3781 tggtcgcggt gttgttcgcc gaagcgcacg aatgggccga agcacactgg accggcgcgc
     3841 atgtacgcat gctcgatccc acgcgcgggg gcaatccgtc gttgcgcaag gtctcggtgc
     3901 acgtgccggc ctacaacgaa ccgccggaca tgctgatcga gacgctcgat gcgctggcgc
     3961 gcatgaacta cccgaatttc gaggtcgtgg tcgtggacaa caatacccgc gatgaggccg
     4021 tgtggcgacc ggtggaagcg cattgccaga aactcggcga gcgcttccgc ttcttccatg
     4081 tcgcgccact ggcgggtttc aaggccggag cactcaacta tgcgctggcg cggacggcac
     4141 cggacgccga agtggtcgcg gtcatcgaca gcgactacaa agtggagccg aactggctgt
     4201 cggatctgtt gccggcgttc gacagtcctc gcatggcgat cgtgcaggca ccgcaggatt
     4261 atcgcgacga caacgacaat gtcttcaagg cgatgtgcta cgccgaatat cgcggcttct
     4321 tttacattgg catgatcacg cgcaacgagc gcaacgcgat cattcagcac ggcacgatga
     4381 cactggtacg gcgttacgtg ctgtcagcgg tcggaggctg ggccgagtgg tgtattaccg
     4441 aggacgccga gctcggcctg cgcgtattcg aggccggcta cgaggcgatc tacatcgcgc
     4501 aaagctacgg ccgcggtctg atgccggaca ccttcgttga ctataagcgc cagcgctttc
     4561 gctgggccta tggcgcgatg cagattctgc ggcatcatgc gagcgccctg ctgcagccgt
     4621 cggcgagtgc actgaccgcg gggcagcgct atcactttat cgccggctgg ctgccgtgga
     4681 tcgccgacgg cttcaacctg ttgttcaata tcgccgcggt gggctggtcg atcgcgatga
     4741 tcgtggcaca ccccaagatc gatccgccgc tgatggtgtt ttcagtgctg ccgctgtcgc
     4801 tgttcatgtt caagctggtg aagctggtac acctgtatac caaccgggtc ggaatgaatg
     4861 ggcgacaaac cgcggcggca gccatttcag gtctggcgct ggcgcacacg atcggacgag
     4921 ccgtgctcaa gggcctgatc agcggtaacg agcctttctt tcgtacgcct aaacgcgtcg
     4981 caccccatcg tttctttgct gcgctggggg cggcgcgtga ggaaaccttg ctgatgctcg
     5041 ctctgtgggc ctcagccctg gggatacacc tgctgccgaa ggcaatggcg agcccggacc
     5101 tgacggtgtg gctggccgcg ttgctgatcc agtcgatccc ctacaccgcc gcactgctgg
     5161 tgtcactcgc cagcgccttc catctgccgg catcgatcat cggtcatcgc cgccagggct
     5221 cggtcaatac acctgccgca caagagtgag cagatgccgt tccgccttga gctgcggaat
     5281 gtga
//
LOCUS       DATMRM010000261         2730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2932078, whole genome shotgun sequence.
ACCESSION   DATMRM010000261 DATMRM010000000
VERSION     DATMRM010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2932078"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VNZ06_07655"
     CDS             complement(<1..228)
                     /locus_tag="VNZ06_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20664.1"
                     /translation="MQEGVALKSLRDRPFELLAELERRGRAVTASATTDVAQAGEWVG
                     VALRMAGELYLVAREEAREVLGIPAPLTRVPG"
     gene            complement(230..595)
                     /locus_tag="VNZ06_07660"
     CDS             complement(230..595)
                     /locus_tag="VNZ06_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW20665.1"
                     /translation="MALILIVDDSPTEVHVMQKALERHGYRTAVAADGAEGIRLARQM
                     HPDLIFMDIVMPGMNGYQATRTLVNDPDTRAIPIVMVTSKGQEADRVWGLRQGAVDYL
                     IKPVSPDQLVEKAQATLAA"
     gene            complement(600..1028)
                     /locus_tag="VNZ06_07665"
     CDS             complement(600..1028)
                     /locus_tag="VNZ06_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW20666.1"
                     /translation="MESTAEDRGGSSTQSGSSLFKGLKVLVIDDSKTIRRTAETLLAK
                     EGCEVFTAVDGFDALAKIADHQPDIVFVDIMMPRLDGYQTCALIKHNKVFRSIPVIML
                     SSKDGLFDRARGRIVGSEHYLTKPFTKDELLGAIESHIGR"
     gene            1227..2195
                     /gene="gshB"
                     /locus_tag="VNZ06_07670"
     CDS             1227..2195
                     /gene="gshB"
                     /locus_tag="VNZ06_07670"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HWW20667.1"
                     /translation="MGNDVRLVVVMDPIDRIKYAKDTTLALLLAAQARGWSLSYLEQR
                     DLRLRDGVAEGRARRLTVRADPAGWFTLGEPAVEPLKAFDVVLMRKDPPFDTEFIYTS
                     YILERAEVDGALVVNRARGLRDMNEKVYTAWFPQCCAPTLITRDMEEMAAFARTHGKI
                     VCKPLHSMGGHSIFAIEPGDKNTRVVFETLTHDGTRFAVVQKYLPDIALGGDSRVLLI
                     DGEPIPYALARIPSADDHRGNLAAGAKGVGRALNDRDRWLCQQIGPALREAGMLFVGL
                     DVIGGYVTEINVTSPTGVRELDSAYGLDIGSMLIDAVARRLSKRRA"
     gene            2217..>2730
                     /locus_tag="VNZ06_07675"
     CDS             2217..>2730
                     /locus_tag="VNZ06_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20668.1"
                     /translation="MTASLQLKRGAAGSAVREGRAPVRERLMAMLFLTALLHAIVILG
                     LTFTATGHGSNDDSPQLDVLLVTNEVPQAQSNEHAAYLSQRTQLGSGNTDAPSPVRSP
                     SSQGAANARSARGEGNGSERGEQALDKSLLNSTDPSAEIRYVGSTANAGGAQPLPQFI
                     GDMPGNPRSGR"
BASE COUNT          524 a          908 c          805 g          493 t
ORIGIN      
        1 acccgggacg cgtgtcagcg gcgcgggtat gcccaatacc tcgcgtgctt cctcgcgcgc
       61 caccaagtaa agttcccctg ccatccgaag cgcaactccc acccactcac ccgcctgtgc
      121 cacatccgtc gttgcactgg cggtgacagc gcgaccgcgt cgctcgagct ccgctaatag
      181 ttcgaacggc cgatcacgca gactcttgag cgctacaccc tcttgcatgt caggcggcta
      241 gcgtcgcctg tgccttctcg accaactggt cgggcgacac cggcttaatc agataatcga
      301 ccgcaccctg ccttaaaccc cacacgcggt cggcctcctg cccttttgaa gtcaccatca
      361 caatgggtat cgcgcgcgtg tcaggatcgt tcaccagcgt gcgcgtcgcc tgatagccgt
      421 tcatccccgg catcacgata tccatgaaaa tcaaatccgg atgcatctgc cgcgcgagtc
      481 taatgccttc agcaccatcc gccgcaactg ctgtgcggta cccgtggcgc tccagggcct
      541 tctgcatgac gtgcacttcg gtcggcgagt catccacgat aaggatcaag gccatcccat
      601 catctcccga tgtgcgactc gatagctccc agcagctcgt ctttggtaaa gggcttcgtc
      661 aggtaatgct cggaaccaac gatgcgcccg cgagcgcgat caaacaagcc gtccttggac
      721 gacagcatga tcacgggaat ggaccggaac accttgttgt gcttgatgag cgcacaggtc
      781 tgatagccgt ccaggcgcgg catcatgata tccacgaaga cgatatcggg ttgatgatca
      841 gcaatcttcg ccagcgcgtc gaagccgtcc accgcggtga acacttcgca gccctccttc
      901 gccagcagag tctctgccgt gcgtcggatg gtcttactat catcgatcac cagaaccttc
      961 agccccttga agagcgagct gccactttgc gtggacgagc cacccctatc ctccgctgtc
     1021 gattccatct ttccagatcc tccgccacgc cccaatcact gacagatcca gacccgcagc
     1081 aggcgcagcc ctgatatgca caattcgccg tgggttttaa catatcagcc accccctcgc
     1141 tatgccgtct ccgtcacgtt tttaagagct cagcagcgcg cggcggaaca taattgctga
     1201 aggtgcgcgc taaccaagga gcaacgatgg gcaacgacgt tcggctggtt gtggtcatgg
     1261 acccgatcga tcgaatcaaa tacgccaaag acaccacgct ggcgctgctg ctggccgcgc
     1321 aagcgcgcgg ctggtcgctc agctacctcg aacagcgtga tctgcggctg cgagacggtg
     1381 tggccgaagg ccgagcacgc cggctgaccg ttcgagccga tccggcgggc tggttcacac
     1441 tcggggagcc agcagtggaa cccctcaaag ccttcgacgt ggtgctgatg cgcaaggatc
     1501 cgccatttga caccgagttc atctatacct cctacattct cgagcgcgcc gaagtggacg
     1561 gtgcgctggt cgtcaatcgg gcccggggcc tgcgcgacat gaacgagaag gtctatacgg
     1621 cctggttccc gcagtgctgc gcccccactc tcatcacccg cgacatggag gaaatggccg
     1681 catttgcgcg cactcacggc aagatcgtct gcaagccgct gcacagcatg ggcggacatt
     1741 cgatctttgc gatcgagccg ggcgacaaga acacgcgggt ggtgttcgaa acgctgacgc
     1801 acgatggtac gcgcttcgcg gtcgtgcaga agtatctgcc cgacattgcg ctcggcggcg
     1861 actccagggt gctgctgatc gatggcgagc cgataccgta cgcactggcg cgcatcccga
     1921 gcgcggatga tcaccgtggc aatctggcgg ccggagccaa gggcgtcggt cgagcgctga
     1981 atgaccgcga ccgctggctc tgccagcaga tcgggccggc gctgcgcgaa gcgggcatgc
     2041 tgtttgtcgg gctggacgtg atcggcggct acgtgaccga aatcaacgtc accagtccga
     2101 ccggggtacg cgaactggac agcgcttatg ggctggacat tggttccatg ctcatcgatg
     2161 ccgttgcgcg acgcctgtcc aagaggcgcg cgtagcggac cgctatactg ctgcgcgtga
     2221 cggcctcttt gcagctcaag cggggcgcgg cgggttcggc ggtgcgcgaa ggtcgcgctc
     2281 cggtccgaga acgcctgatg gcgatgctgt tcctgacagc attgctgcac gcgatcgtga
     2341 ttctcggtct gaccttcacc gccaccggac acggcagcaa cgacgattca ccgcaactgg
     2401 acgtgctgct ggtcaccaat gaggtgccgc aagcgcagtc caacgagcac gccgcctacc
     2461 tctcccagcg cacgcagctc ggcagcggca acaccgatgc gccctccccg gttcgcagtc
     2521 cgtcgagcca gggggccgcg aacgctcgct cggcccgggg cgaaggcaac ggcagcgaac
     2581 gcggcgagca ggcgctcgat aaatcgctgc tcaattcgac cgatccgagc gctgaaatcc
     2641 gctacgtcgg atccacggcc aatgccggcg gcgcgcagcc actgccgcag ttcatcggcg
     2701 atatgccggg caatccgcgc agcggccgcg
//
LOCUS       DATMRM010000262        11677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2932221, whole genome shotgun sequence.
ACCESSION   DATMRM010000262 DATMRM010000000
VERSION     DATMRM010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11677
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2932221"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VNZ06_07680"
     CDS             <1..549
                     /locus_tag="VNZ06_07680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWW20669.1"
                     /translation="VALASDLHGNRQQVFDRERIQALLGDLRGNPARIRARAQGALDA
                     LCACPEVDTQRIAAIGFCFGGTMALELARSGALISAVVGFHSGLLTAAPQDAKNIRGR
                     VLVCIGAEDPLIPPQQRADFETEMRAGNVDWQMSLYGGVVHSFTNPAADRMNRPEALR
                     YDAKADARSWQQMCELFDEVFQ"
     gene            533..1738
                     /locus_tag="VNZ06_07685"
     CDS             533..1738
                     /locus_tag="VNZ06_07685"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW20670.1"
                     /translation="MKSSNSAPATSMPAVDLVYFNAGGGHRAAALALEASIRELGIPW
                     QVRLVNLTEILDPKDAFKSAMGFAPEDFYNRRLAHGWTFGLAQELKILQGIIRLAHKP
                     MTRKLRRHWVKTRPDMVVSLVPNFNRAMYHSLATALPGVPYVTVLTDMADHPPNFWIE
                     RGQAQHLICGTAKAVAQARAAGYPDGHIHATSGMIIRPDFYRPGHGDRRQELRKLGLD
                     PNRITGMVMFGGHGSRVMRDIARQLNHTQLILICGHNRSLAKKLRSMSFAAPRLVLEF
                     TSEIQRYMRLSDFFIGKPGPGSISEAVQQQLPVIVVRNSWTMPQERYNTQWVREHNVG
                     LVLPSFRSVRAAVQQLDGHLEEFRAAIRQIHNRAVFEVPEILQGILNATKPVRRQVLR
                     SSLDVAAAT"
     gene            complement(1696..2604)
                     /gene="asd"
                     /locus_tag="VNZ06_07690"
     CDS             complement(1696..2604)
                     /gene="asd"
                     /locus_tag="VNZ06_07690"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HWW20671.1"
                     /translation="MNLVRAVSKILQQEDINFLLCNRIPRLWMTRFFGWYSQIERAWL
                     VRGSIALWRMFSALELHEAKKTRFNSLHDCFTRELKDGARPINKDQSVLTSPCDGIVG
                     AIGGVEEESVLQAKGFPYRLIDLLGDPELVRYYARGSYATLRLTSGMYHRFHAPHDCC
                     VEQVNYVSGDTWNVNPIALKRVENLFCRNERAIIRCRLETTGDLITLVPVAAILVASI
                     RLHFLDVLLHLKYRGPNVIECDHPLLKGEEMGWFQHGSTILVFAPEGFSFCSNIHEGS
                     IVRVGQPLFTTSRHVAAATSSDERST"
     gene            2649..4364
                     /locus_tag="VNZ06_07695"
     CDS             2649..4364
                     /locus_tag="VNZ06_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWW20672.1"
                     /translation="MTTPTILFALAGAAIVAFLWRPLRQRLALSRAKHRSLAGHARLS
                     RAFSKWVPFYEYDEAHFFNADGAPEGVAQQRRDGFYKLAALYAQRFVKTARATSEVQS
                     EIADLQFTSRYRVPPQFSRIVRQNLSAGSFLQSSSGTTTTDLDGNQFYDLTGSYGVNV
                     FGYDFYKECIARGSERVRDLGPVLGAYHPVTAFNVHRLREISGLDEVSFHMSGTEAVM
                     QAVRLARYHTGRRHLVRFCGSYHGWWGDVQPGVGNPLPARDTYTLKDMAEASLRVLRM
                     RSDIACVLVNPIQAMHPNVNAPTDNMLLDSGRKAHFDRAAYTEWLKKLRALCAERNIV
                     LIFDEVFVGFRIARGGAQEYFGVKADMVTYGKTVAGGLPIGVVCGRRDLMRRYREDRP
                     ADICFARGTFNAHPYVMGAMYEFLTRLDSEQIRAQYHNLDEVWDARAEQLNERLRAAG
                     LNVQVANLSSVWTVFYPQPSRYNWMLQYYLRAQGLSLSWVGTGRIIFSLNYSDADFAQ
                     VAERFVSAAAQMQREGWWWRSEELSNKAIRRRVLRETLRARGSASSGQSAHPAAGTVA
                     LENKA"
     gene            complement(4258..5052)
                     /locus_tag="VNZ06_07700"
     CDS             complement(4258..5052)
                     /locus_tag="VNZ06_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20673.1"
                     /translation="MDPVMLASQSSVKSSTSGRYAPICHISVTKPIYFVGWPLFFAKR
                     TKGTKMHSFLRQFSRQLAIQRWDDHRYYHHSRINQSLHLLSALSFLAAYVTIFRNPAA
                     SALIAWLVAMVSRQAGHFFFEPKGYDDINHATHEHKEDIKVGYNLRRKVVLMAIWALS
                     PAVLWVDPTLFGIFPAGSGRGVFIQHMGQIWLTIGFGGLIFRTVQLFFIRDVMTGLVW
                     CTKIVTDPFHDVKLYFRAPLYLLRGELIDPMMHCRELGESPEAHVV"
     gene            5268..6050
                     /locus_tag="VNZ06_07705"
     CDS             5268..6050
                     /locus_tag="VNZ06_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HWW20674.1"
                     /translation="MNRLIREAAFSLGYNLTPKIPDADVLGLISSLRPRQCGRPLIRV
                     GADRDGGYLIPDDLEGIEYCFSPGVNELSAFENQLADRRIRSFLADYSVSGPPYPRPE
                     FIFDRKHIGANDNGTFMTLASWKERYLPGYSQDLLLQMDIEGGEYEALLSTPDEMLKQ
                     FRIAVIEMHWLERLFDPFAFRIMNACFQKLLSRFYVVHLHPNNCGGCVREESIEVPHV
                     MEITFYNRERAPPGEYCTQFPHPCDADNCSGLPTLPLPACWH"
     gene            6151..6702
                     /locus_tag="VNZ06_07710"
     CDS             6151..6702
                     /locus_tag="VNZ06_07710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWW20675.1"
                     /translation="MSTRFRTVSGALLLGVMLVAATGLRLAGTSQDSLQAGPVPQEVP
                     PPPEPQPLPAWKPAPDRTGRTRVGVASFYADFFAGRVMADGGIMDPGSDNAASRTLPL
                     GTTAKVTNLSTGQSAVVTIEDRGPYAKHRLLDLSPATAEKIGITRGKGIARVKVSPIA
                     VPMPDGSVKAGVAASDPEIVMLR"
     gene            complement(6784..8355)
                     /locus_tag="VNZ06_07715"
     CDS             complement(6784..8355)
                     /locus_tag="VNZ06_07715"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWW20676.1"
                     /translation="MPFQSNPLVAKVSEGVMANLLFDHIIVLMLENRSFDHIYGYLGL
                     GEGLKASGMVNYLTPGKSGTQAFRTRKGGDYLAVGDGPSHSLKEVNLQLFGNTKQPAS
                     VAAATAKLNGFAASFRTALFEKFKREPTSNEIQQVMNCFDPTQLPVLTSLAQNFVLCD
                     HWFADVPGPTMPNRAFVHAATSQGYTYNASWKPKFTCDTLYDRISAQPGLCWRVYYFD
                     LNDVLELYPNVKSDPTNHVLFEQNFLSDVAGDTLPTYSFITPAFLGKNHITINSMHAP
                     ADVRPAEKLIADVYGALKSNEQVWKKTLLVITFDEHGGYYDHVVPPSTVSPDGIPGRL
                     DQSFLVPFDFKRLGLRVPALLISPWFPAAVDSTVYSHSTIPGSVIDAFQLPGGFLTAR
                     DENANKLTQKYLLSSANRTWRTNTPDLTVPVQPQALDAMQRDILDGSVNLDPHPDMRD
                     AKRTRDIQDLAHATDFVRTQISKHLEHRFASGGRARLAAKLTAENQLPSDAVSPARVA
                     ELARSAPKGTRRKTR"
     gene            8483..9586
                     /gene="mtnA"
                     /locus_tag="VNZ06_07720"
     CDS             8483..9586
                     /gene="mtnA"
                     /locus_tag="VNZ06_07720"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HWW20677.1"
                     /translation="MNVNGQAYRTIWLADDGISVRVIDQRRLPHEFTTLDLRTLDEAL
                     IAIKEMAVRGAPLIGATAAFAVALALRADPSDAALAASVDRLVACRPTAVNLAWAART
                     VASQVMRLPTKQRWAGALAEAQRICEDDVSCCRAIGAHGADLLASLDTGGRPIQILTH
                     CNAGWLATVDWGTALSPIYVAQQRGLSLHVWVDETRPRNQGAALTAWELGQQGIPHTV
                     IADNAGGYLMRQGRVDLCIVGADRVAGNGDVCNKIGTYLKALAAFDNRVPFYVAMPHS
                     TIDWNLSCADHITIEQRDPDEVRSIVGLDANGKLSRVQLTPPGSAAANFAFDVTPARL
                     VTGLITERGIARATRESLAGLYGIGSAVKALPQ"
     gene            9583..11634
                     /locus_tag="VNZ06_07725"
     CDS             9583..11634
                     /locus_tag="VNZ06_07725"
                     /inference="COORDINATES: protein motif:HMM:NF006192.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HWW20678.1"
                     /translation="MRSLWSAEDAVNLVRHYAARGVAEDVALRIYSSRLLGSDPRLVQ
                     HGGGNTSVKTRERLITGEQVEVIRVKGSGWNLDAIEPEGLPSMRLSALQELKHVAHMS
                     DEAMVNAQRSALLDSNAPTPSVETLLHAWVPQVVIDHTHANAVLAVVDQPDGEKRAGD
                     MFADVLAICPYVMPGFALAQKVREIIEAQRTAQGIILLKHGIFTWGETAREAYERMIA
                     MVTRAERAIEQASGSEPRVMASSHAPEKLAARVLPVLRGALAQSLGAGAWKRVVLQRR
                     ESPSIMSYLQHPQLQQISQHGPATPDHVLRIKPWPLILRGLGESSEAQLAASVREAVN
                     EFAQRYQAYFARNNRRVGGDRTALDPMPRVILAPELGLIGVGQRHTDAATAADVAQTN
                     AEVISAAQSLGSFVSIDEGDVFDVEYWSLEQAKLGKSITGRLAGHVVAITGGGGALGS
                     ATARAFFREGAEIVVLDSNAQAASTVASAVQGLGLTCDVTDASAVEGAMHAVCRTYGG
                     LDILVSNAGAAWTGRIGEVDDSTLRASFELNFFAHQNAARAAVAIMRRQQTGGVLLFN
                     ASKQALNPGTSFGPYGIPKAAVVALAKQYALDYGSMGIRANVVNADRIRSGLLTQQMI
                     AERAQARGVGEAEYMGGNLLGLEVRASDVAETFLALALAERTTAAIVTVDGGNVAASV
                     R"
BASE COUNT         2257 a         3426 c         3729 g         2265 t
ORIGIN      
        1 gtggcgctgg cgagcgatct gcacggcaat cgtcaacagg tatttgatcg ggaaagaatc
       61 caggcgttgc tgggtgatct gcggggtaat ccggcgcgta tccgcgctcg cgcccaaggg
      121 gcgctcgatg cgctgtgcgc ctgccccgag gtcgatacgc agcggatcgc tgccatcgga
      181 ttctgttttg gaggcacgat ggcgctggaa ctggcgcgca gtggtgcgtt gatctcggcc
      241 gtcgtggggt ttcactcggg gctgctcacc gcggctcccc aggatgcgaa aaacatccgc
      301 ggccgagtgc tggtgtgtat cggtgccgag gatccgttga ttccaccgca acagcgcgct
      361 gactttgaaa ccgagatgcg tgctggcaac gtcgactggc agatgagcct gtacggcggt
      421 gtggtgcata gttttaccaa tccggcggcc gacaggatga atcggcccga ggccctgcgc
      481 tacgatgcga aagctgacgc acgttcgtgg caacagatgt gcgaactgtt cgatgaagtc
      541 ttccaatagc gcgcctgcga cctcgatgcc agcggtcgat ctggtctatt tcaatgccgg
      601 cggcggacac cgggctgcgg cactggccct ggaggcctcg attcgcgagc tggggatccc
      661 ctggcaggtg cgactggtca acctgacgga gattctcgat ccaaaggatg cgttcaagag
      721 tgcgatggga ttcgcgccgg aagacttcta caatcgccgt ttggcccatg gctggacctt
      781 cggtctggcg caggaactga agatcctgca agggatcatc cggctggcgc acaaaccgat
      841 gaccaggaaa ttgagacgac actgggtcaa gacgcgaccc gacatggtcg tctcgctggt
      901 gccgaacttc aatcgcgcca tgtatcactc actggcgacg gcattgcctg gagtgccgta
      961 cgtgaccgtc ctgacagaca tggcggatca tccaccgaac ttctggatcg agcgcggaca
     1021 ggcgcagcat ctgatttgcg gcactgccaa ggcggtcgcg caggcacgcg ccgcgggcta
     1081 tccggacggc cacatccatg cgacttccgg catgatcata cgacccgatt tttatcggcc
     1141 ggggcacggc gatcgccgac aggaactgcg taagctgggt ctggacccga accgcatcac
     1201 cggtatggtg atgttcggcg ggcacggctc gcgcgtcatg cgcgacatcg cgcgccaact
     1261 caatcacacg caactgattc tgatctgcgg acacaaccgg agtctggcga agaagctgcg
     1321 ctcgatgtcg tttgccgcac cgaggctggt gctggaattc accagcgaga tccagcgcta
     1381 catgcggctg agcgactttt tcatcggcaa acccggacct ggcagcatca gtgaggccgt
     1441 acagcagcag ctaccggtca ttgtggtgcg aaactcctgg accatgccgc aggagcgcta
     1501 caacacacag tgggtacgcg agcacaatgt cggcctggta ttgccgagct tccgctcggt
     1561 gcgtgcagcc gtgcaacagc tcgacgggca cctggaggaa ttccgggcgg caatccgtca
     1621 gatccacaat cgcgcggtgt tcgaggtgcc agagattctc cagggtatcc tcaacgccac
     1681 caagcccgtt cgccgtcagg tgctgcgctc gtcgctggat gtcgctgccg ccacatgacg
     1741 cgaggtggtg aatagcggct ggccgacgcg aacgatcgat ccttcgtgga tgttgctaca
     1801 gaaggaaaat ccttccggcg caaataccag gatcgtggag ccgtgctgga accagcccat
     1861 ctcctcaccc ttcaacagcg ggtggtcgca ctcgatgacg ttcggaccgc ggtacttcag
     1921 atgcagcagc acatcgagga agtgcaggcg aatgctggca accaggatcg cggcgaccgg
     1981 taccagcgtg atcagatcgc cagtagtctc cagccggcag cgaatgatcg ctcgctcatt
     2041 gcggcagaac agattctcga cgcgcttcag ggcgatcgga ttcacattcc aggtatcgcc
     2101 tgacacgtaa ttgacctgct cgacacaaca atcgtgcggt gcatggaagc ggtggtacat
     2161 gcctgatgtc agacgcagcg tcgcgtagga gccgcgtgcg tagtaacgca ccagctccgg
     2221 gtcgcccagc agatcgatca gccgataggg gaagcccttg gcctgcagca ccgactcttc
     2281 ttcgacccct ccaatcgcgc cgacgatgcc atcgcaggga ctcgtcagaa cgctctggtc
     2341 cttgttgatg gggcgcgcgc catccttgag ctcgcgggtg aagcagtcat gcagactgtt
     2401 aaatcgcgtc ttctttgctt cgtgcagctc gagcgctgag aacatgcgcc ataacgcgat
     2461 gctcccgcgc accagccagg cacgctcgat ctggctgtac cagccgaaaa agcgcgtcat
     2521 ccaaagtcgc ggaatacggt tgcacagcag gaagttgatg tcctcctgct gcagaatctt
     2581 ggaaaccgct cgtaccaggt tcattgtagt gtcatgggtc tgcaatatga ttgtcttgga
     2641 ggatcgccgt gaccactccg acaattctgt ttgctcttgc tggcgccgct attgtcgcat
     2701 ttttgtggcg tccgttgcga caacgactgg cattgtcgcg cgcgaaacac cggtcgctgg
     2761 ccggccatgc acgcctgagc cgcgcattct cgaaatgggt acccttctac gagtacgacg
     2821 aggcgcattt cttcaacgct gatggtgccc ccgagggcgt ggcgcagcaa cggcgtgatg
     2881 gcttttacaa actcgctgcc ctgtatgcgc agcgtttcgt caaaacggct cgcgccacca
     2941 gcgaggtgca atcggaaatt gccgatctgc agttcaccag ccgctaccgg gtgccacccc
     3001 agttcagccg catcgtgcgg cagaacctca gcgccgggtc ctttctgcaa tcgtcgtccg
     3061 ggaccacaac gaccgacctg gatggcaatc agttctacga tctgaccggc tcctatggcg
     3121 tcaacgtctt cggctacgac ttctacaagg aatgcatcgc gcgcggcagc gagcgcgtgc
     3181 gtgacctggg gccagtgctc ggtgcctacc atcctgtcac agcattcaac gtgcatcgtc
     3241 tgcgcgaaat atccgggctg gacgaagtat ccttccacat gtccggaacg gaagctgtga
     3301 tgcaggcggt gcgcctggcg cgctatcaca cagggcgccg gcacctggtg cgattttgcg
     3361 gctcgtatca cggttggtgg ggcgacgtgc agccgggggt gggaaacccg ctgcctgcgc
     3421 gcgacacata cacgttgaag gacatggctg aggcgagcct gcgcgtattg cgaatgcgca
     3481 gcgacatcgc ctgcgtcctg gtcaacccga tccaggcgat gcatcctaac gtcaacgcgc
     3541 cgactgacaa catgctgctc gatagcggcc gcaaggcgca ctttgaccgt gcagcgtaca
     3601 ccgagtggct gaagaaactg cgtgcgctct gcgccgagcg caacatcgtg ctgatattcg
     3661 acgaagtgtt cgtcggtttt cgcatcgcac gtggcggggc gcaggagtat ttcggcgtca
     3721 aggccgacat ggtgacctac ggcaagaccg tggccggcgg cctgccgatc ggtgtggtct
     3781 gcggcaggcg tgacctgatg cgccggtacc gcgaggatcg cccggcagac atctgcttcg
     3841 cccgcggaac cttcaacgcc catccgtatg tgatgggcgc gatgtacgag ttcctgacgc
     3901 gcctggacag tgagcagata cgcgcgcagt accacaatct ggatgaggtc tgggatgcgc
     3961 gggccgaaca gctcaatgaa cgtctgcgcg cggcgggcct caacgtacaa gtcgcgaacc
     4021 tctcctcagt ctggacggtg ttttatccgc agccctcgcg ctacaactgg atgctgcagt
     4081 attacctgcg tgcccagggc ttgagcttga gctgggtggg taccggtcgg atcatcttca
     4141 gtctcaacta cagcgatgcg gactttgcgc aggtcgccga acgcttcgtt tcggcggctg
     4201 cgcaaatgca acgcgaaggc tggtggtggc gcagcgaaga gctgagcaac aaggccatca
     4261 gacgacgtgt gcttcgggag actctccgag ctcgcggcag tgcatcatcg ggtcaatcag
     4321 ctcaccccgc agcaggtaca gtggcgctcg aaaataaagc ttaacatcgt ggaacgggtc
     4381 ggtgacgatc ttggtgcacc acaccagtcc ggtcatcacg tcacggatga agaacagttg
     4441 cactgtgcgg aagatcagac cgccaaaacc gatagtcagc cagatctggc ccatatgctg
     4501 aatgaagacg ccacggcctg aaccggccgg gaagatgccg aacagcgtcg gatccaccca
     4561 cagcaccgcc ggcgacagcg cccagattgc catcagcacg accttacgcc tcaggttgta
     4621 gcctaccttg atgtcttcct tgtgttcgtg agtcgcgtga ttgatgtcgt cgtagccctt
     4681 cggctcaaag aagaagtgtc ctgcctgacg actgaccatc gcgaccagcc aggcgatcag
     4741 cgccgaggcc gcgggattcc taaagatcgt cacgtacgct gccaggaagc tgagcgcgct
     4801 cagcaggtgc agggattggt tgatacggct gtgatggtag tagcggtgat catcccaacg
     4861 ctgaatcgcg agctggcgcg aaaactggcg caagaagctg tgcattttgg ttcccttggt
     4921 gcgtttggca aaaaaaagcg gccagccaac gaaatatatc ggtttcgtta cgctgatatg
     4981 acagattggc gcataacggc ctgaagtgga agattttacg ctcgattgtg acgcgagcat
     5041 cactggatcc aacccaaatc tcgccctcga cgatgtggac gcgtcaatga agtcgtttga
     5101 gtcactgaac gcgagttcga ccttgacgga cagtcgtcat tcgcccgcta tcgccgaaca
     5161 cgaccctgcc cggatgcaaa tggccagagg gccatgaggc tgcgccctgt accgcgctac
     5221 cggcattggc gcactattgg ggtaaagaac agcagggaga cttggacttg aaccggttga
     5281 ttcgggaggc ggcattttcc ttaggctaca acctcactcc caagattccg gacgccgacg
     5341 tgctggggct gatcagtagt ctgcgtccgc gccaatgcgg ccgtccgttg atccgggtgg
     5401 gcgcggaccg ggacggcggg tatctgattc cggacgacct ggaaggcatt gagtactgct
     5461 tttcgcccgg cgtgaatgag ttgtcagcgt tcgagaatca gctcgccgat cgccggattc
     5521 gctcgtttct ggccgattac tcggtcagcg ggccgccgta cccgcggccc gagttcatct
     5581 tcgatcgcaa acatatcgga gccaacgaca acggcacctt catgaccctg gcgtcgtgga
     5641 aggagcgata tctgcccgga tacagccagg atctgctgct acagatggat atcgagggcg
     5701 gcgagtacga ggcacttctc agtactccgg atgagatgct gaagcagttc aggattgctg
     5761 tcatcgagat gcactggctc gagcgtctat tcgatccctt cgcgttccgg atcatgaacg
     5821 cctgctttca gaaactgctc agccggtttt acgtggtgca tctgcatccg aacaactgcg
     5881 gcggctgcgt gcgcgaagag agtatcgagg taccgcacgt gatggagatt actttctaca
     5941 atcgagaacg cgcacccccg ggagagtatt gcacgcagtt cccgcatcca tgcgatgcgg
     6001 acaactgttc aggattgccg acactgccat tgcctgcctg ctggcattaa caccggaata
     6061 ttcctacagt ttggcgccga accattcgga cagtccgcag cgtcgtcctg ccgacagatc
     6121 catcgctgtc ccaaccgcca agatgacagc atgtctacgc gctttcgaac tgtcagcggc
     6181 gctctattgc tcggcgtcat gctggtcgcg gccaccggat tgagactggc cggtacgtcg
     6241 caggacagct tacaggccgg ccctgtgccc caggaggtgc cgccgcctcc ggaaccgcag
     6301 ccgttgccgg cgtggaagcc ggcgcctgat cgcactggcc ggacgcgcgt gggagttgcc
     6361 tcgttttacg cggacttctt tgcggggcgt gtgatggcgg atggagggat catggatccg
     6421 ggtagcgata acgccgccag tcgaacgttg ccgctgggca cgacggccaa agtgaccaat
     6481 ctatcgaccg gtcagagcgc cgtcgtgacc atcgaagatc gcggaccgta cgccaagcat
     6541 cgactcctgg acctgtcgcc ggccaccgcc gagaagatcg gcattacacg cggcaagggt
     6601 attgcgcgtg tcaaggtctc gccgatcgcc gtgcctatgc ccgatggcag cgtcaaagcg
     6661 ggtgtcgcgg ccagcgaccc cgagatcgtg atgctacgct gaactggctt cggttcggtt
     6721 catcgctacg acaggggtcc ggcccgaggt tgcggcgctc gcagacccga ggcgcagacg
     6781 agactagcgg gtcttgcgac gagttccttt cggtgcagac cgtgcgagtt cggcgacccg
     6841 cgccggactg actgcgtctg aagggagctg gttttctgcg gtaagtttcg ctgccagtcg
     6901 tgcgcggccg ccggatgcga atcgatgttc gaggtgcttg ctgatttgcg tgcgaacgaa
     6961 atccgtggcg tgtgcgagat cctggatgtc acgcgtacgc ttggcgtcgc gcatgtccgg
     7021 atgcggatcc agattcaccg agccgtccag aatgtcgcgc tgcatggcgt caagtgcctg
     7081 cggctgcacc ggcaccgtca ggtcaggcgt gttggtacgc caggtccgat tggcagacga
     7141 gagcagatat ttctgcgtga gcttgttcgc gttctcatcg cgggcggtga gaaagccacc
     7201 cggcaactgg aacgcatcga tcacggatcc agggatagtc gaatggctgt agacggtgga
     7261 gtccaccgcc gccgggaacc atggcgagat cagcagggcc ggcacccgaa gacccagccg
     7321 cttgaagtcg aacggcacta gaaacgactg atcgaggcgc cccggaatgc catccggcga
     7381 taccgtcgag ggtgggacga catgatcgta gtagccgccg tgctcatcga acgtgatcac
     7441 cagcagcgtc ttcttccaca cctgctcgtt ggacttcagc gctccgtaga cgtcagcgat
     7501 cagtttctcc gccggacgca catcggccgg tgcgtgcatg ctgtttatgg tgatgtggtt
     7561 ctttccaaga aacgctgggg tgatgaacga ataagtcggc agggtgtcgc cagcgacgtc
     7621 actgaggaag ttctgttcga acagcacgtg gttggtagga tcggacttga cgttgggata
     7681 gagctccagc acgtcgttca agtcgaagta gtacaccctc cagcaaaggc caggttgtgc
     7741 actgatgcgg tcgtagagcg tgtcgcaggt gaatttcggc ttccagctgg cgttgtaggt
     7801 gtaaccctgc gaggtggccg cgtgaacgaa ggctcgattg ggcatggtcg ggcccggaac
     7861 atcggcgaac cagtggtcgc acagtacgaa gttctgtgca aggctcgtca atacgggcag
     7921 ctgagtcgga tcgaagcagt tcatgacctg ctggatctcg ttggaggtcg gttcgcgctt
     7981 gaatttttcg aacagtgcgg tgcgaaagga ggcggcgaag ccgttcagct tcgcagtggc
     8041 ggcagcgaca ctggccggct gcttggtgtt gccgaacaat tgcagattga cctctttcag
     8101 gctgtgcgag ggaccatcgc cgaccgcgag gtaatcgccg cccttgcgcg tcctgaaggc
     8161 ctgagtgccg ctctttccgg gagtcaggta gttgaccatg ccgctcgcct tcaagccctc
     8221 tccgagtccg agatagccat agatgtgatc gaacgatcgg ttctccagca tgaggacgat
     8281 gatgtgatcg aatagcagat tggccatgac accctccgag actttcgcta ccagcggatt
     8341 gctttgaaat ggcacaggaa aagccgctgt tgatggcgcg accttagccc gcgaacgaac
     8401 tttcgaacac tcgcccgctg tgaattattt gttgcagact tactgattcc atagaatcga
     8461 agcagggggc ggcgattgct caatgaacgt caacggtcaa gcgtacagaa caatttggtt
     8521 ggcggacgat ggcattagcg tgcgtgtcat cgatcagcga cgcctgccgc acgagttcac
     8581 gacgctggac ttgcgcacgc tcgatgaagc gctgatcgcg atcaaggaga tggccgtgcg
     8641 cggagcgccg ttgatcggcg ccactgcagc gttcgccgtg gcattggctc ttcgggccga
     8701 tccttcggat gcggcgctgg cggcatccgt cgaccgactg gtcgcctgtc gccccacggc
     8761 cgtgaatctg gcgtgggcgg cgcgcacggt cgcatcgcag gtcatgcgtc ttccgacaaa
     8821 gcagcggtgg gctggggcgt tggccgaagc gcagcgtatc tgcgaggacg atgtgagctg
     8881 ttgccgcgcc atcggggcgc acggcgcgga cttactggca agcctcgaca ccggcgggcg
     8941 tccgatccag atactgactc actgcaatgc cggctggctg gcgacggtgg actggggaac
     9001 cgcactctcg ccgatctatg tcgctcagca acgcgggctg tcgcttcacg tttgggtcga
     9061 tgagactcgg ccgcgcaatc agggcgcggc gttgactgct tgggagctgg gtcagcaagg
     9121 catccctcat acggtcatcg ccgacaatgc cggcggttac ctgatgcgcc agggcagagt
     9181 cgatctgtgt atcgtcggag ccgatcgtgt cgcaggcaat ggcgatgtct gcaacaagat
     9241 cggcacttac ctcaaggctc tggccgcctt cgacaatcgc gtgccgttct atgtggcgat
     9301 gcctcattcg accatcgact ggaatctgag ctgcgcagac cacatcacga tcgagcaacg
     9361 cgaccccgac gaggtgcgaa gcatcgtggg cctcgatgca aacggtaagc tctcgcgggt
     9421 gcagctcaca cctcccggct ccgccgccgc gaattttgct tttgacgtga caccggcacg
     9481 tctggtgacg ggcctgatca ccgagcgcgg tatcgcacgc gccacacggg aaagtctcgc
     9541 tggtctgtat ggcattggct cagcagtgaa agcgctaccg caatgaggag cctgtggagc
     9601 gctgaggacg ccgtcaatct cgtcaggcac tacgcggcca ggggtgtcgc ggaggatgtg
     9661 gcgctgcgca tctattccag ccgcctgctc ggctccgacc cgcggctggt gcagcacggt
     9721 ggcggcaaca cctcggtgaa gacgcgggaa cggctcatca cgggtgagca ggtcgaggtc
     9781 atccgcgtga agggcagcgg ctggaacctg gacgccatcg agcctgaagg cctgccatcc
     9841 atgcggcttt cggcactgca ggagctgaag cacgtcgcgc atatgagcga tgaggcgatg
     9901 gtgaacgccc agcgctcggc gttactcgat tcaaatgcac ccacgccatc cgttgagacg
     9961 ttgctgcatg cctgggtgcc acaggtcgtc atcgatcata cccacgccaa cgcagtgctc
    10021 gctgtcgtgg accaacctga tggcgagaag cgggcgggcg atatgttcgc ggacgtgctg
    10081 gcaatctgcc cgtacgtgat gcccggcttt gcacttgcgc aaaaggtgcg ggagatcatc
    10141 gaggcacagc gcaccgcgca gggcatcatt ctgttgaaac atggcatctt cacttggggc
    10201 gagacggcac gcgaggccta cgagcggatg attgcgatgg tcacgcgcgc ggagcgggcc
    10261 atcgagcagg cctctggctc cgagccacgc gtcatggctt cttcgcacgc acccgaaaag
    10321 ctcgcggctc gcgtgctgcc ggtattgcgc ggagcgctgg cgcaaagtct aggagctggc
    10381 gcctggaagc gtgtggtgct gcaacggcgc gagtcgcctt cgatcatgag ctatctgcag
    10441 catccgcagt tgcagcaaat cagtcagcac ggacctgcga ccccggatca tgtgctgcgc
    10501 atcaagccgt ggcctctgat actgcgcggt ctcggcgagt cttccgaggc gcagctggcg
    10561 gcgagcgttc gcgaggcagt gaatgaattt gcgcagcgat accaggcgta cttcgctcgc
    10621 aacaatcgac gtgtgggtgg cgatcgcacg gcactggatc cgatgcctcg cgtcattctg
    10681 gcgcctgaac tggggctgat cggagtcggt cagcgacaca ccgacgccgc aacagcggcg
    10741 gatgtcgcgc agacgaacgc ggaagtcatc tcggcagcgc agagtctcgg gtcattcgtg
    10801 agcatcgacg aaggcgacgt attcgatgtg gagtactggt cgctggaaca ggccaagctt
    10861 ggcaagtcca tcaccggtcg gctcgccgga catgtcgtgg ccatcaccgg tggtggcggg
    10921 gcgctgggat ccgcgaccgc acgagcgttc ttccgcgagg gggccgaaat cgtcgtgctg
    10981 gattccaacg cgcaggcggc cagcaccgtg gcgagcgcag tgcagggtct cgggctgacc
    11041 tgcgacgtga ccgatgcgag cgcggtcgag ggcgcgatgc acgcggtctg tcgcacctat
    11101 ggtggactgg acatcctggt ttccaacgcc ggagcggcct ggacgggccg catcggcgag
    11161 gtcgatgact cgacgcttcg ggcgagcttt gagctgaatt tcttcgccca tcagaatgcg
    11221 gcacgggcgg cggtcgcgat catgcggcgt cagcagaccg gtggcgtatt gcttttcaat
    11281 gcctcgaagc aggccctgaa tccagggacg agctttggtc cctatggaat tcccaaggcc
    11341 gcggtcgtgg cgcttgcgaa gcagtacgct ctcgactacg ggtcgatggg tatccgagcc
    11401 aatgtcgtga atgcggatcg gatccgatcg ggactgctga cgcagcagat gattgccgag
    11461 cgcgcccagg cacgcggtgt gggcgaggcc gaatacatgg gcggcaacct gctgggcctg
    11521 gaggtgcgcg ccagcgacgt cgccgagaca tttcttgcgc tggcgctggc cgagcgcacc
    11581 actgccgcga tcgtcacagt cgatggcggc aacgtggcgg cgtcggtccg atgaatgcaa
    11641 ccccatatgg ctggagagcc cttccatgaa agactgg
//
LOCUS       DATMRM010000263         8043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2627876, whole genome shotgun sequence.
ACCESSION   DATMRM010000263 DATMRM010000000
VERSION     DATMRM010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8043
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2627876"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VNZ06_07730"
     CDS             <1..1122
                     /locus_tag="VNZ06_07730"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HWW20679.1"
                     /translation="IAEGVRTVKSLSALLDETDADPTAAARTARARGQRVIGFANADV
                     PVELIDASGCFALSLMHCAGGATPHADRYLEASFSPLDRSIAETWLSGKLELLDAVVF
                     SRGSDSSQRLYYYLCELQRRQLTRGPRPLLFDLAKIPRASSIAYSYEATRELAAALGV
                     QAERLPLSIARRNRRRALLQSLQRLRAEARPPRGTLIERISRLADCCEADELDTALAH
                     WMREPRPAFSGPRLLLSGSAPRDGTLHAVVEQAGGAIIAESGDHDLERLGSPLAADST
                     DPLRALSEHYQALPHGARAFIDRAADLRRRLESSRAHAVIFWLLEEEEALVWDLPPQQ
                     QALQASRVPALTLTRQPSTINEQTLLSLRRFVAQLASPG"
     gene            1119..3290
                     /locus_tag="VNZ06_07735"
     CDS             1119..3290
                     /locus_tag="VNZ06_07735"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWW20680.1"
                     /translation="MNTLQGHRVLALGDTAAGALARLLRSFGAQIRPTTLQELKDALA
                     RASFLLEDRGLALLERSGWTRARIEAINPGLIHVSVTCFGSAGPRAEWHGSELIASAT
                     GGALRLTGQPDRAPVKEALDACLFHADMVAAAGLMAAHYERGQSGRGQHVDIAAQEVA
                     LSRNVSGILVWQFDRRKLSRVGGALNYGRATVRCIWALADGWCFHSLMTGRFGAPANQ
                     ALSDWIDEAGLDNPLRGTDWLRYDRSSLDPQLRLRWEAAIEAFFRTRSRSQVTHDGRR
                     RGINATVLASPADVLADPHLQARDFWRQEPARRTPAHFVRFEAGSQAAPCAHPPTARP
                     GPLSGVRVLDFSWALVGSITTKVLGDLGAEVIKVESRTRPCLSRLDVQVAASSARSLD
                     DKPWFAHLNSSKKSLSIDLKIPASREVIAPLIAWADLVVENFSPGTMAKLGLAFPQLQ
                     QINPDIILVSGSVFGQTGPLAQEWGVDGTGAALSGRTLLTGWPDRDPVIPGAVPYGDV
                     IVPYVMVAAALAVLQHRRERGGGGHIDAAMYEICVQQMYAALIAAQTDQPPQRRANRD
                     ASFYHQGVYPVAGDDRWIAISCRTQADWQCLSRISGADEHGSDAQREQQLAAWSATRS
                     GEQLVETLQAAGIAAGLAEDIEDLLEHDPQVKARESLTALDHAQLGKFGHVRTPISFS
                     RSLIAPYCAPRIGEHNEWIARRLAGLSAERYEAFVRREVFK"
     gene            3287..4573
                     /locus_tag="VNZ06_07740"
     CDS             3287..4573
                     /locus_tag="VNZ06_07740"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HWW20681.1"
                     /translation="MSPSRERHSRKDLRCTAEATAFQRRFASTLRERVVERGEPFVLA
                     QADTPHELFHAMDIALITNQWWSAYISAKQLSARYFAALEQHGFPNNSCHYCSLGLAC
                     SLANDPATAPWGGLPTPTVLVARLTCDCIQQVFGQWARLYGTDFFPLEAPGWIHKDPN
                     WFEKSNDAWEQVYEDKRIALLVEEMRELIGLLERKTARRFDERRLEALMHSINEQESY
                     IAEAAQMIGSARPCPVSIADQMPNTMIPQWHRGSEWALAHSRRFRDEVAERVSTGTGA
                     AANERVRLMWIGAGLWHDPGFYNALEERHGAVFVWSMYTPFAGPQYIRDLKGQPMRAL
                     ASRICSMNEVLHLPPWMNGWMVSEASRCGIHAAVMLVPPGNRLSQSGTALTALALEKA
                     GVPTLTLTADMVDAKDWSREHTVELVSQFLRRSGLA"
     gene            4570..5601
                     /gene="dctP"
                     /locus_tag="VNZ06_07745"
     CDS             4570..5601
                     /gene="dctP"
                     /locus_tag="VNZ06_07745"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HWW20682.1"
                     /translation="MNLKFALALILLSASAAAAATELTYASPYGPNHPFSLADRTWIH
                     WVEQRSGGRLHIVPIWAGALLSSDQSMLELRHHVADIGLITPIYTRGGAQLIRTQAGF
                     YDGARSFAQQVAMYRCLEASSPEFARELNGLTVLAVQGGTLPGVLTRTRRVTTLDGLR
                     GLRIRAPTELLGVLRELGADPVEMPMGDVYSALAKGVLDGVVAPADTLRSLHFAEVAK
                     HFWQLEVPRGAYPARAMATDRWQRLSADDRAVLTAARSVWEQALDTQTQAAVQSGQAM
                     GRLQGVEFVPATPEQQARFDELYQQDAARNALALRRYDISANETLTLARRITDGIRRT
                     GSVSCGANP"
     gene            complement(5646..7031)
                     /locus_tag="VNZ06_07750"
     CDS             complement(5646..7031)
                     /locus_tag="VNZ06_07750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW20683.1"
                     /translation="MASDASAATPLAATVSEQWRPYQFYALVLLTLIVVLNYLDRYSL
                     SLLQESIKHDLKLSDWQLGLLSGPAFTLFYSVGGIVLARYAERLHRGTILAAALGVWS
                     TMTALCGAAGNFAQLALCRLGMGIGEGGCIPINLSLATETFSIRQRGLVMAIIGAGNP
                     ISGIITPLLIAAVARAFGWRDAFVAIGLPGVVIALLVHFTLRDPRARARGSVQHQATT
                     SLVADARVLFGNPAFLLLFVAAIFTGMAVSGVGAFRVSYLMRTQGMDLTHAGAIWSIA
                     SAAGLLGAVAGGYLADHFADERGRSYPIVAAISLMLTFAGYFIGFTQSDPAVVMVALI
                     AGSFTYNMKDGPVYAAVQNVVPKRMRTTGAALYMFAATSLGGASGPMLAGGVSDLVGA
                     HHVHATLSAYAATCPGGRALPGVAANIGLCAQASADGLRWGLALLSAVFALAAIFDAL
                     ASRSMHAREPA"
     gene            complement(7031..>8043)
                     /locus_tag="VNZ06_07755"
     CDS             complement(7031..>8043)
                     /locus_tag="VNZ06_07755"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HWW20684.1"
                     /translation="YHEELSRYGALTLAVLLDEGREDKPWGGLPKPSLICTGIADRSA
                     QIMADYSGAVFVPLEMPVLRHPHPQWWQMSRWQWEDLDESPRIDVMLAQLRDLVAASE
                     RVAGRKLDLDRLREILARVNRQEEYFDRIREIIATAAKLPARLGEVMSQVMGIQWHRG
                     TQWALDQARAFHDEVKQRADRQLWVCPNERYRLMYVGQGLWQNLDFFTGFEQRYGAVF
                     VRSNYLSIAADGYLRYPVRDPLRALASRYVTMSEQMHIPGLGGAWATWEARRHRVDGG
                     LSIDRWWGARLINLALEEQGIPILNLTVDPVDGNTWDEAALSQQVSQFIEQRIIPARE
                     RG"
BASE COUNT         1415 a         2693 c         2619 g         1316 t
ORIGIN      
        1 attgccgaag gcgttcggac tgtgaagagc ctcagcgccc tgctggatga aaccgacgca
       61 gatccgaccg ccgcggcgcg cacggctcga gcgcgcggcc agcgcgtcat cggcttcgcc
      121 aatgccgatg tgccggtcga actgatcgat gccagcggct gctttgcgct gtcgctgatg
      181 cactgcgccg gcggcgcgac accgcatgcg gatcgctatc tggaagccag cttctcaccg
      241 ctcgaccgct cgattgcgga aacctggctg tccggaaaac tggagctgct ggatgcggtc
      301 gtgttctcac gcggatccga cagctcgcag cggctctact actatctgtg cgagctgcaa
      361 cgacgccagc tcacacgcgg accgcggccg ctgctgttcg atctggcgaa gatcccccgc
      421 gccagcagta tcgcttacag ttacgaggcc acgcgcgaac tcgccgccgc gctcggtgtt
      481 caggccgaac gactcccctt aagcatcgcg cgccgaaacc gccgccgagc actgttgcag
      541 tcgctgcagc gactgcgcgc ggaagcccgg ccaccgcgcg gaaccctgat cgaacgcatc
      601 agccgactgg cagactgctg tgaggcggat gaattggata ccgccctcgc tcattggatg
      661 cgcgaaccac gaccggcctt ctcaggcccg cggctgctgc tgtccggcag tgcgccccgc
      721 gacggcacgc tgcacgccgt ggtggaacaa gccggcggcg cgattatcgc cgagtcgggc
      781 gatcatgacc tcgagcggct gggttcgccg ctcgccgctg acagcaccga tccgctgcgg
      841 gcgctgtccg agcactatca ggcgctgccc cacggcgcgc gcgcgttcat cgatcgtgcc
      901 gctgatctgc gccgccggct cgagagctcg cgcgcgcacg cggtcatctt ctggctgctg
      961 gaagaggaag aggccctggt gtgggacttg ccgccacagc agcaggcgct gcaggccagt
     1021 cgcgttcccg cgttgaccct gacgcgccag ccctcaacga tcaacgaaca gacgctgctg
     1081 agcctgcggc ggttcgtcgc ccaactagcg agccccggat gaacacgctc caggggcatc
     1141 gggtactcgc gctcggagac accgccgccg gtgcactcgc acggctgctg cgctcgttcg
     1201 gcgcgcagat acggccgacg accctgcaag agctgaagga tgctctcgcg cgcgcatcct
     1261 tcctgctcga ggaccgcggt cttgccctgc tggaacgcag cggctggacc cgcgcgcgca
     1321 tcgaggcgat caacccggga ttgattcacg tgtcggtcac gtgttttggc agcgcaggcc
     1381 cgcgcgccga atggcacggt tccgagttga ttgcctccgc caccggtggc gcgctgcggc
     1441 tcacgggcca gccggatcgc gcaccggtga aagaagccct cgatgcctgc ctgtttcacg
     1501 ccgacatggt ggcggcggcc ggcctgatgg ccgcacacta cgaacgcggg caatccggac
     1561 gcgggcagca cgtggatatc gccgcacagg aagtggccct gtcgcgcaat gtcagtggca
     1621 tcctggtgtg gcagttcgat cgccgcaagc tgtcgcgtgt cggcggcgca ctcaactacg
     1681 gccgggcaac ggtgcgctgc atctgggccc tggccgatgg ctggtgtttt cacagcctga
     1741 tgaccggtcg cttcggcgcg ccggccaatc aggcgctgag cgactggatc gacgaggcag
     1801 ggctcgacaa cccgctgcgc ggcaccgact ggctgcgcta tgaccgctcg agcctggacc
     1861 cgcagctgcg cttgcgctgg gaggcggcca tcgaggcgtt ttttcgcacg cgcagccgct
     1921 cgcaagtgac gcacgacggc cggcgccgcg gcatcaatgc gacggtgctg gcctccccgg
     1981 ccgacgtgct ggcagaccct cacctgcagg cgcgcgattt ctggcgacag gagccggcgc
     2041 ggcgtacacc ggcacacttc gttcgtttcg aggccggctc gcaagccgcg ccgtgcgcgc
     2101 atccacccac tgctcgcccg ggccccttga gcggtgttcg ggtgctggac ttctcctggg
     2161 cgctggtagg ctcgatcacc accaaagtgc tcggtgatct cggagccgag gtcatcaagg
     2221 tcgagagccg cacccggccc tgtctgtcgc gcctggacgt gcaggtcgcc gcctccagcg
     2281 cacgcagcct cgacgacaag ccgtggtttg cgcatctgaa cagttcgaag aagagccttt
     2341 cgatcgatct gaaaattccc gcgagccgcg aagtcatcgc gccgctgatc gcctgggcgg
     2401 atctggtggt cgagaatttc tcaccgggca cgatggccaa gctcggcctg gcattcccgc
     2461 agctgcagca aatcaacccc gacatcattc tggtttccgg cagcgtgttc ggacagaccg
     2521 gcccgctggc gcaggagtgg ggcgtcgatg gcacgggcgc tgcgctctcg ggacggacac
     2581 tgctgaccgg ctggccggat cgcgatccgg tcatcccggg ggcggtgcct tacggggatg
     2641 tgatcgtgcc atatgtgatg gtcgccgccg cgctcgcagt gctgcaacat cgacgtgaac
     2701 gcggtggtgg cggccatatc gacgcggcaa tgtatgaaat ctgcgtgcaa cagatgtacg
     2761 ccgcgctgat cgcggcgcaa accgaccaac ccccccagcg ccgcgccaat cgcgatgcca
     2821 gcttctatca tcagggtgtc tatccggtgg caggggatga tcgctggatc gccatttcat
     2881 gccgcacgca agccgactgg cagtgtctga gccggatcag cggcgccgat gagcacggca
     2941 gtgatgcgca gcgcgagcag cagctggcgg cctggtcagc cacgcgctca ggcgagcaac
     3001 tggtggaaac cctgcaggcg gcgggcattg ccgccggcct tgccgaggac atcgaggatc
     3061 tgctcgagca tgacccgcag gtcaaggcgc gtgaatcgct gactgcgctg gatcacgcgc
     3121 agctggggaa attcggacac gtgcgcacgc cgatcagttt ctcacgcagc ctgatcgccc
     3181 cgtattgcgc tcctcgcatc ggcgagcaca acgaatggat cgcgcgacgc cttgcgggac
     3241 tatcggccga gcgctacgag gccttcgtgc gccgggaggt attcaagtga gcccttcgcg
     3301 cgagcgccac agccgcaagg acctgcgctg cacggccgaa gcgaccgcct tccagcgccg
     3361 ctttgccagc acgctgcgcg agcgggtggt cgagcgcggt gagccgttcg tgctcgcgca
     3421 ggccgatacg ccacacgagc tgtttcacgc gatggacatt gcgctgatca ccaatcagtg
     3481 gtggtcggcc tatatctccg ccaagcagct ctcggcgcgc tatttcgcgg cgctcgagca
     3541 gcatggtttc ccgaacaaca gctgtcacta ctgctcgctg ggactggcct gcagtcttgc
     3601 caacgatccg gccactgccc cgtggggagg cctgccaaca ccgacggtac tggtcgcccg
     3661 gctgacctgc gattgcattc agcaggtgtt tggtcagtgg gcgcgtctgt acggcacgga
     3721 ttttttcccg ctcgaggcac cgggctggat ccacaaagat ccgaactggt tcgagaagtc
     3781 caacgatgcc tgggagcagg tgtacgagga caaacgcatt gcactgctgg tggaggagat
     3841 gcgtgagctg atcgggctgc tcgagcgcaa gaccgcaagg cggttcgacg agcggcggct
     3901 cgaagcactg atgcattcga tcaacgagca ggagagctac atcgccgaag cggcgcagat
     3961 gatcggcagc gcgcgcccct gccccgtgtc gatcgccgat caaatgccga atacgatgat
     4021 cccgcagtgg caccgcggct ccgagtgggc actcgcgcat tcgcggcgct tccgggatga
     4081 agtggccgag cgcgtcagca ccggcaccgg cgcagcggcc aacgagcgcg tgcgcctgat
     4141 gtggatcggt gccggactgt ggcatgaccc gggtttctac aacgcgctcg aggagcgcca
     4201 cggcgcggtg tttgtgtggt ccatgtatac gccgtttgcc ggcccgcaat acatccgcga
     4261 tctcaagggt cagccgatgc gggcgctggc cagtcgcatc tgttcgatga atgaagtgct
     4321 gcatctgccg ccgtggatga acggctggat ggtgagcgag gcttcgcgct gcggcatcca
     4381 tgccgccgtc atgctggtgc cgccgggcaa tcgactgtca cagtccggta ccgcgctcac
     4441 ggcgctggcg ctggaaaaag ccggcgtgcc cacgctcact ctgacggccg atatggtcga
     4501 tgcgaaggac tggagtcgtg agcacacggt ggaactcgta tcgcagtttc tgcgtcgctc
     4561 gggcctggca tgaaccttaa gttcgcgctg gccctgatcc tgctcagtgc cagcgcggcg
     4621 gccgcggcaa ccgagctgac gtatgccagt ccctacggtc ccaatcatcc attcagcctc
     4681 gcggacagga cctggatcca ctgggtcgag cagcgctcgg gcgggcgatt gcacatcgtg
     4741 cccatctggg cgggcgcgct gctgtcttcg gaccagtcga tgctcgaact gcgccaccac
     4801 gtcgccgaca tcggtctcat cacacccatc tacacgcgcg gcggcgcgca actgattcgc
     4861 acacaggccg gcttctacga tggcgcccgc agctttgcgc aacaggtcgc gatgtatcgg
     4921 tgcctggagg cttcctcgcc cgagttcgcg cgcgaactga acggcctgac agtgcttgcg
     4981 gtacaaggcg gcacgctccc cggggtgctc acgcgcactc ggcgcgtcac gacgctggat
     5041 gggctgcgcg gactgcgcat ccgtgcgccc accgaactgc tgggcgtgct gcgcgaactc
     5101 ggcgccgatc cggtcgaaat gccgatgggc gatgtctact cggcactggc caagggtgtg
     5161 ctcgatggcg tggtggctcc ggccgacacg ctgcgctcgc tgcactttgc cgaggtggcc
     5221 aagcacttct ggcaactcga agtcccgcgt ggcgcctatc cggcgcgggc gatggccact
     5281 gatcgctggc aacgcctgtc ggccgacgat cgtgccgtgc tgacggcggc gaggtcggtc
     5341 tgggagcagg cactggacac gcaaacccag gcagcggtgc agtccggaca ggcgatggga
     5401 cgtctccaag gcgtcgagtt cgtacctgca acgcccgaac agcaggctcg attcgatgag
     5461 ctgtaccagc aggatgccgc acgaaacgca ttggccctcc ggcgctacga catttccgca
     5521 aatgagacgc tcacactcgc gcgccggatc acggacggta ttcgtcgcac gggtagcgtg
     5581 agctgcggtg cgaacccgtg agcgatgcca cggccggcct gatggtggcg ggagcgggta
     5641 ttctcctacg ctggctcgcg cgcatgcatg ctgcggctgg ccagcgcatc gaagatcgcc
     5701 gccagcgcga acaccgcact cagtaacgcc agtccccagc gcaatccgtc agcagaagcc
     5761 tgtgcgcaca atccgatgtt ggcagcgaca ccgggtagcg cacggcctcc cgggcaggtt
     5821 gccgcatacg cgctgagcgt ggcatgaaca tggtgagccc cgacgagatc gctcacgccg
     5881 ccggccagca tcggaccgct ggcaccgccc aagctcgtgg ctgcaaacat gtagagcgcc
     5941 gccccggtgg tgcgcatgcg cttggggacg acgttctgga ccgcggcata caccgggccg
     6001 tccttcatgt tgtaagtgaa tgaaccggcg atcagcgcaa ccatcaccac cgctggatcc
     6061 gactgtgtga aaccgatgaa atagcccgca aaggtcagca tgagactgat cgcggccacg
     6121 atcggatagg agcgtccgcg ctcgtcagca aagtgatcgg ccagatagcc tccggctacc
     6181 gcaccgagca aaccggcggc gctcgcaata ctccagatgg caccggcgtg agtgagatcc
     6241 attccctggg tgcgcatcag ataggacacc ctgaaggcgc cgacaccgct gaccgccatc
     6301 ccggtaaaaa tcgcggcgac gaacagcaac aggaacgccg gattgccaaa cagcacccgc
     6361 gcatcggcta ccaacgaggt cgtggcctgg tgctggaccg aaccgcgcgc gcgggcacgc
     6421 gggtcgcgca acgtgaaatg caccagcagc gcaatcacca cgccgggcag gccgatggcg
     6481 acgaatgcgt cgcgccagcc gaacgcccgc gcgaccgccg caatcagcag tggcgtgatg
     6541 atgccggaaa tcgggttgcc ggcaccgatg atcgccatga cgagaccacg ctggcggatg
     6601 ctgaaggtct ctgtcgcgag cgacagattg atcggaatgc agccgccttc cccgatgccc
     6661 atgcccagac ggcatagcgc gagctgggcg aaattgccgg ccgccccgca cagtgcggtc
     6721 atggtcgacc acaccccgag cgccgcggcg aggatcgtgc cccgatgcaa ccgctcggca
     6781 tagcgtgcga gcacgatgcc gcccacggag tagaacagcg tgaaggccgg acccgacagc
     6841 aggccaagct gccagtcgct gagcttcaga tcgtgcttga tggattcctg cagcagcgac
     6901 agcgagtagc ggtccaggta gttcagcacc acgatcagcg tcagcagtac cagtgcgtag
     6961 aactgatagg gacgccactg ctcggatacc gtggcggcga gcggtgtggc cgcggaggcg
     7021 tcgctggcca tcagcctcgc tcgcgcgccg gaatgatgcg ctgctcgatg aactgcgaga
     7081 cttgctgcga cagcgcggcc tcatcccagg tgttgccgtc caccggatcc accgtcaggt
     7141 tgaggatcgg aatgccctgc tcctccagcg ccagattgat caggcgcgcg ccccaccagc
     7201 gatcgatcga cagtccgccg tcgacacggt gccgccgtgc ctcccatgtc gcccaggcgc
     7261 cgcccagccc gggaatgtgc atctgctcgg acatcgtcac gtagcgactg gcgagtgccc
     7321 gcagcggatc gcgcaccggg tatcgcagat aaccgtcggc cgcgatcgac agatagttgg
     7381 agcgcacgaa caccgcgcca tagcgctgct cgaacccggt gaagaagtcg aggttctgcc
     7441 acaggccctg cccgacgtac atgagccggt accgttcatt cggacacacc cagagctgcc
     7501 gatcggcgcg ttgtttgacc tcatcatgga aagcacgcgc ctgatccagc gcccactgtg
     7561 taccgcgatg ccactgaata cccatgacct ggctcatcac ctcaccgagc cgggccggca
     7621 gtttggcggc tgttgcgatg atctcgcgta tccgatcgaa atactcctcc tggcgattca
     7681 cgcgcgcgag gatctcgcgc aaccgatcca gatcgagctt cctgccggcc acacgctcgc
     7741 tggcggcaac cagatcgcgc agctgcgcga gcatcacgtc gatacgcggc gattcatcca
     7801 ggtcttccca ttgccagcgc gacatctgcc accactgcgg atgcggatgg cgcagcacgg
     7861 gcatctccag cggcacgaac acagcgccgc tgtagtcggc catgatctga gcgctgcgat
     7921 cggcgatgcc agtgcagatc agcgagggct tgggcagccc tccccacggc ttgtcctcgc
     7981 gcccctcatc cagcagcacc gccagcgtca gcgcgccata gcggctgagc tcctcgtgat
     8041 aac
//
LOCUS       DATMRM010000264         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3863055, whole genome shotgun sequence.
ACCESSION   DATMRM010000264 DATMRM010000000
VERSION     DATMRM010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3863055"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VNZ06_07760"
     CDS             complement(<1..297)
                     /locus_tag="VNZ06_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20685.1"
                     /translation="MNHDTHKRDFSINARLPGISALAAGIGALSTLAAFALLSLIHLF
                     TNLFFFGSFSFADRSPALNTLGAWVVVIPAAGGLIVGLMARFGSEKIRGHGIPEA"
     gene            complement(304..717)
                     /locus_tag="VNZ06_07765"
     CDS             complement(304..717)
                     /locus_tag="VNZ06_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWW20686.1"
                     /translation="MKVITRGLAKADFEQLSEFRYQMRRFERFSEQAAQGEGITPLQY
                     LLLLHIKGYPDREWATVGELAERLQAQHHGVVALVSRCEALELVTRKISETDRRQVEV
                     HLLKAGEKVLARLAELHRAELKSLKGAFTIPQIDL"
     gene            complement(794..1894)
                     /locus_tag="VNZ06_07770"
     CDS             complement(794..1894)
                     /locus_tag="VNZ06_07770"
                     /inference="COORDINATES: protein motif:HMM:NF016843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HWW20687.1"
                     /translation="MRACVGALLGIGFTGTVMHLLLGPVGSNPLLVAPMGASAVLLFA
                     VPSSPLAQPWSIIGGNLVSATVGVLCAQWIADPVVASALAVSLAICAMFALRCVHPPS
                     GAVALTAVLGGPAVHALGYGFVLAPIAMQSAALLSAALVYHAITGHRYPHAAHVVHIR
                     SGTDAATASEQTRGGVTRADIEAVLNRRGELLAIDPDDLESLLREAEMRTYARTFSET
                     LCADVMSKSVISISPTTTVKAASNLLKRQGIKALPVTDAARQVVGIVTRADLAGNPTG
                     ARKSPTESLSARWFTGTPNPDATVDTVMSTDVCSIEAATPIVELVPLFADYGHHHIPV
                     LDARRQLAGIITQADLISGLYRQTQAQQRQVA"
     gene            2454..>2651
                     /locus_tag="VNZ06_07775"
     CDS             2454..>2651
                     /locus_tag="VNZ06_07775"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWW20688.1"
                     /translation="MGLPASAAPAPGHVGAPSPHRPALFALSLAALGVVYGDIGTSPL
                     YTLKTVFDPANGLPLDAGNVIG"
BASE COUNT          511 a          826 c          859 g          455 t
ORIGIN      
        1 ggcttccggg atgccgtgac cgcggatttt ctccgagccg aagcgcgcca tcagcccgac
       61 gatgaggccg cccgctgccg gaatcaccac gacccacgcg ccgagcgtat tgagtgccgg
      121 ggagcgatcg gcgaacgaaa agctgccgaa gaagaacagg ttcgtgaaca gatgaatcag
      181 gctgagcagc gcgaacgcgg cgagcgtgct gagtgcaccg attcccgctg caagcgcgga
      241 aataccgggc agccgcgcat tgatcgagaa gtcgcgcttg tgcgtatcgt ggttcatgac
      301 gggttacaaa tcgatctgtg gaatcgtgaa cgcgcccttg agcgacttga gttcggcacg
      361 atgcagttcc gcgagccgcg cgagcacttt ctcgcccgct ttcagcagat gcacttcgac
      421 ctgacgccgg tcagtctcgc tgatcttgcg cgtcaccagt tccagcgcct cgcaacgcga
      481 gaccagcgcg accacgccgt gatgctgcgc ctgcaaccgc tcggccagtt cgccgacagt
      541 cgcccactcg cgatccgggt agcccttgat gtgcagaagc agcaggtatt gcagcggcgt
      601 gatgccttcg ccctgcgcgg cctgctcgga aaagcgctcg aagcgccgca tctggtaacg
      661 aaattccgac agctgttcga agtctgcttt cgccaacccg cgtgtgatga ctttcatccg
      721 tgatccgcca ttgatgaaga caggttaaac ggcgaatata tcacaaggtg atacatattc
      781 cgtcgttaga caatcatgca acctgtcttt gctgggcctg ggtctgccgg tacaggccgg
      841 agatcaggtc tgcctgcgtg atgatgcccg cgagctggcg cctggcgtcg agcacgggaa
      901 tgtgatggtg gccgtaatcc gcgaagaggg gcaccagttc gacgatcggc gtcgcggcct
      961 cgatcgagca gacatcggtg ctcatcaccg tatcgactgt cgcatcgggg ttgggcgtgc
     1021 cggtgaacca gcgcgcggac aggctttccg tcgggctctt tcgcgcgccc gtcgggttgc
     1081 ccgccaggtc cgcgcgggtg acgatgccga ccacctgtcg cgctgcgtcc gtgaccggca
     1141 atgccttgat gccttgccgc ttcagcaggt tcgacgcagc tttgaccgtg gtagtgggcg
     1201 agatgctgat cacgctcttc gacatcacgt cggcgcagag ggtctcgctg aaggtgcgtg
     1261 cataggtgcg catctcggct tcgcgcagca gcgattccag atcgtccgga tcgatcgcga
     1321 gcagctcgcc gcggcggttg agcacggcct cgatgtccgc tcgggtgacg ccgccgcgcg
     1381 tctgttcgct ggctgtagcc gcgtcggtgc ccgaacggat gtggacgaca tgtgctgcgt
     1441 gcggatagcg atgacccgtg atggcgtgat agacgagcgc ggccgagagc agcgccgccg
     1501 actgcatggc aatcggcgcc agcacgaagc cgtagccgag cgcgtgcacg gccggaccgc
     1561 cgagcaccgc ggtcagcgca accgcgcccg aaggcggatg cacgcagcgc agcgcgaaca
     1621 tcgcgcagat cgcaagcgag actgcgagcg cagaggcgac gaccgggtcc gcgatccact
     1681 gcgcgcacag cacgccaacc gtcgccgaca cgagattgcc gccgatgatc gaccacggct
     1741 gcgccagcgg gctggagggc acggcgaaca gcagcacggc agacgcgccc atcggcgcga
     1801 ccagcagcgg attgctgccg accggaccga gcagcagatg catgaccgtg ccggtgaagc
     1861 cgatcccgag cagtgcgccg acgcaagcgc gcatacgctc ttcccagcgg acggtgagat
     1921 taccaggcgt aaagcgggca agccaggaca gtgtgcgaac gagtatcgag cggagcaagg
     1981 tcggtttggt cagaagaaag cgacgccagt taaaagagtc gtcaccttaa atgaaagctg
     2041 gaattaaggg aaagaacttg ttctcatgaa tgtatgcgat cgtgatataa atcgacgatt
     2101 gccggagagg gctgccggtc tggataccgc cgtgaaagcg cgtccgcgcg cgtaagagcg
     2161 ccatctggag tgcccaagcc aggtccagtt acatctcacg aggtgatata agtactcgtg
     2221 gaaaacccca tgagaatccg ggttttcgcg cactgcgtca ccggtttttt tgccgccgtt
     2281 ttctgcgccg cttccctata cttcagcaac acggccagca cgcagcacga cctgacactc
     2341 ctgcaagatt cgggctcgcg cggcacaacc ggaacgacaa tccgtaccca cgcgggcaaa
     2401 gcgcgcagcg ctgcgcggct tctgccggcg caggcgtacg tctggaggga gacgtgggac
     2461 tgccagcatc cgctgcaccg gcccccgggc atgtcggcgc gccgagtccg cataggccgg
     2521 cgctgttcgc cctttcgctc gctgcgctcg gcgtggtgta cggcgacatt ggcacgagtc
     2581 cgctgtatac gctgaaaacc gttttcgacc cggccaacgg acttccgctc gacgcgggca
     2641 acgtcatcgg c
//
LOCUS       DATMRM010000265         2888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3226826, whole genome shotgun sequence.
ACCESSION   DATMRM010000265 DATMRM010000000
VERSION     DATMRM010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3226826"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1952
                     /gene="asnB"
                     /locus_tag="VNZ06_07780"
     CDS             <1..1952
                     /gene="asnB"
                     /locus_tag="VNZ06_07780"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HWW20689.1"
                     /translation="VHRGPDDCGYYIQRDVGLGFAHRRLSVVDLSPAGHQPMSSHGGR
                     YQIVFNGEIYNHLEIRRELQARCDARAWRGHSDTETLLAAIETWGLQAALQRSVGMFA
                     LALWDRERRRLMLARDRVGEKPLYYGRSGQAFLFASELKALQAHPAFKPEIDHDCLAL
                     YLRYCYVPEPYSIFQGIRRLPPGTTLEVDARGHCDPPVSYWSAAAITDVPQSKKFTGG
                     DREALDALDRLLSGAVGQQMIADVPLGAFLSGGIDSSLIVALMQKQSSRRVRTFTIGF
                     TEPAYDESQYARAVARHLGTEHTELLVTPAEAMEVIPRLSSIYDEPFADSSQIPTFLV
                     SQMARQHVTVALSGDAGDELFGGYNRYAWAKRLSGVMSFQRPVRKLTARSIRALSPDT
                     WARLFGYARAFLPSRWQAAQMGDKLHKFAELIDCSRTELYRTLVSHWPAPAQVVIGAV
                     EPRTLLTDLMAESGNRGFEECMMYWDLMTYLPGDILVKVDRAAMAVSLETRVPMLDHR
                     VIEFAWSLPLKMRVRAGEGKWLLKRLLSRYVPPALTNRPKTGFGIPIDSWLRGPLRGW
                     AEELLDESRLRGEGFFNPAPIREKWRQHVDGRRNWAYWLWDILMFQAWWDGQQQLRGR
                     SDAEAVTLGHGVRTESRAVARQPPL"
     gene            2005..>2888
                     /locus_tag="VNZ06_07785"
     CDS             2005..>2888
                     /locus_tag="VNZ06_07785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW20690.1"
                     /translation="MKIMFFVRSMGVGGAERQLCVLCRELLRRGHEVSVLLYYGGEQL
                     EDELRDLGVRIINLKKRGRWRNLDFLARLIRTVRAEQPDVLYALLPVSNLLALLLRHV
                     GGGCAIVCGVRASDVSLPKLDWLIRLTAQLERQLVRHADVVIVNSRTGARYLCGERHF
                     PNLVVVDNGIETQTFSFNESGRRRMRAAWQARDDTPIVGCVARLDPLKDHVTLLRAFA
                     LLRHSHPDARLICVGSVIEPYATELRHLARQLQIDAAIVWLERESQLRDLYSAFDTLC
                     LSSISEGFPNVLAEAMACG"
BASE COUNT          508 a          857 c          948 g          575 t
ORIGIN      
        1 tggttcacag ggggccagat gactgcggct attacattca aagagacgta gggctgggct
       61 tcgcgcatcg acggctgtcc gtcgtcgatc tgtctcccgc cggtcatcag ccgatgagct
      121 ctcacggcgg gcgctatcaa atcgtgttca acggcgagat ctataatcac ctggagattc
      181 ggcgcgagct gcaggcgcgc tgcgacgcgc gggcgtggcg cgggcactcg gacaccgaaa
      241 cgctcctggc ggccatcgaa acctggggac tgcaggctgc gttgcagcgc tccgtgggta
      301 tgttcgcgct ggcgctctgg gaccgcgagc ggcgcaggct gatgctggcg cgcgatcggg
      361 tcggggaaaa accgctctac tatggacgct cggggcaagc cttcctgttt gcatcggagt
      421 tgaaggccct gcaggctcat ccggcattca agcccgagat cgatcatgac tgcctggccc
      481 tatatttacg gtattgctac gtacccgagc cgtattccat ctttcaaggg atccgtcgac
      541 tgccgcctgg aactacgctg gaggtggatg ctcgcggtca ctgcgatcca ccggtcagtt
      601 actggagcgc agcggcaatc acggatgtcc cgcagagcaa gaaattcact ggcggcgatc
      661 gcgaggcgct ggatgcgttg gatcgtctgc tgagcggagc cgtgggtcag cagatgatcg
      721 ccgatgtgcc gctaggagct ttcctgtcgg gcggcatcga ctcctcgctg atcgttgcgc
      781 tcatgcagaa acagtcttct cgcagggtgc gcacgttcac tatcggattt accgagccag
      841 cctacgacga gtctcagtat gcccgggcgg tcgcgcgcca cctgggcaca gagcacaccg
      901 aactgctggt cacacccgcc gaggcgatgg aggtgatacc gcgtctctcc agcatctacg
      961 atgaaccctt cgcagactcc tcgcaaattc ctacttttct cgtctcgcag atggcgcggc
     1021 agcatgtcac cgtcgcgcta tcaggtgacg caggcgatga gctgttcggg ggctataacc
     1081 gttacgcctg ggcgaagcga ctgtcgggag taatgagctt tcagagaccg gtgcgtaaac
     1141 tgacggcgcg ctctatccgt gcactctctc cggacacctg ggcgcgcctg tttgggtacg
     1201 ccagagcctt cctgccatcg cgctggcaag ccgcgcagat gggagacaag ctccacaagt
     1261 ttgccgagct gatcgactgc tcgcggaccg agctgtaccg gacgctggtt tcacactggc
     1321 cggcccccgc acaggtggtg atcggggcgg ttgagccgcg cacactcttg accgatctga
     1381 tggccgagtc cggcaatcgc ggcttcgaag agtgcatgat gtactgggac ctgatgacct
     1441 acctgccggg ggacatactg gtaaaggtcg accgggccgc gatggcggtg tcactcgaga
     1501 cgcgcgttcc aatgcttgat caccgagtga tcgagtttgc ctggtcgctg ccgctgaaaa
     1561 tgcgtgtacg ggccggcgaa ggcaagtggc tcctgaagag gcttctgagt cgctacgttc
     1621 cacccgcact aacgaacagg ccaaagaccg gttttggcat accgatcgat tcttggctgc
     1681 gcgggcctct gcgagggtgg gcggaggaac tcctcgatga atcgcgcctg cgaggcgagg
     1741 gtttcttcaa tccggcaccc atacgcgaga aatggcgcca acatgtggac gggagacgta
     1801 actgggccta ctggctgtgg gacatcctga tgttccaggc ctggtgggat ggacagcagc
     1861 agctgcgcgg gcgcagcgac gccgaggctg tgacgctggg gcacggcgtc cgtaccgagt
     1921 ctcgggcggt ggcccgccag ccaccgctat gaccgcttcg ttcctttggg tgggcgaagt
     1981 ttcgaggtca cgacgcgcgc acgcatgaag atcatgtttt tcgtgcgctc catgggcgtt
     2041 ggcggagcgg agcgtcaact gtgcgtgctg tgtcgtgaat tactgcggcg tggtcacgag
     2101 gtgagcgtgc tgctctacta cggcggcgag cagctcgagg acgagcttcg cgacctcggc
     2161 gtgcgcatca tcaatctcaa aaagcgcggc cgctggcgca acctggactt tctggcgcgt
     2221 ctcatccgca cggtgcgcgc ggagcagccg gacgtcctct acgcgctgct gccagtctcg
     2281 aatctccttg cgctgctact gcgtcacgtc ggaggcgggt gcgcaatcgt ctgcggggtg
     2341 cgcgcctcgg acgtgagtct gcccaagctc gattggctga tccggctgac tgcgcagctc
     2401 gaacggcagc tggtgcgcca cgcggacgtg gtgatcgtga actcgcggac gggggcgcgt
     2461 tacctgtgtg gcgaacgaca cttccccaac ttggtcgtgg ttgacaatgg aatcgagacg
     2521 cagacatttt cattcaatga atccggtcgc cggcgaatgc gcgcggcctg gcaggcacgg
     2581 gatgacacac cgatcgtcgg atgtgtggca cgcctcgacc cgctcaagga tcacgtgaca
     2641 ctgttgcgcg cgtttgcgct gctgcgacac tcacatcctg atgcacgcct gatttgcgtc
     2701 gggagcgtga tcgagcccta cgcgaccgag ctcaggcatc tagcgcggca attgcagatc
     2761 gatgcagcga tcgtctggct tgagcgcgag tcgcagctgc gcgacctgta cagcgcattt
     2821 gatacgctat gtctcagctc gatctcggag gggtttccga acgtgcttgc cgaagccatg
     2881 gcttgcgg
//
LOCUS       DATMRM010000266         3397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1390585, whole genome shotgun sequence.
ACCESSION   DATMRM010000266 DATMRM010000000
VERSION     DATMRM010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390585"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1203
                     /locus_tag="VNZ06_07790"
     CDS             247..1203
                     /locus_tag="VNZ06_07790"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWW20691.1"
                     /translation="MIPVVLLISLFMLRVFALMHECGHGSLFHTQRLNRGFGFLLGVM
                     SGMPQYVWSQHHNYHHAHNGNWDKYRGPYTTLSVDEYAALSPIRQRMYRCKCSIAGAP
                     IAGFIYLVFNPRFNWLKGSIAFIAHVMRRKLAEPAQSFMVHAASFSTPYWKSVREYRH
                     MAWNNIALLSIWALMCSALGPSLFFKIYLSTLSLAGGAGIVLFTVQHNFRHAYASDEK
                     NWDYDTGAVEGTSFLILPAWLNWFTANIGYHHVHHLSARIPNYRLIACHNEYRHLFSA
                     VTRVKLAQINEALKCILWDSHAQQIISVAEYQQQVKRAPSAA"
     gene            1269..2360
                     /locus_tag="VNZ06_07795"
     CDS             1269..2360
                     /locus_tag="VNZ06_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWW20692.1"
                     /translation="MNARRSTRADSGLHAGVAAALVSALLFGATTPIAKQLLQGATPL
                     LIAGLLYLGSGIGLGLLRLIQDRRWRPIGLRGAQWSWLAAATLTGGVLAPALLMLGLT
                     RSDAATASLLLNLEAVFSAVLAWLIFREATSRRVVLGFVSIFVGGLLLAWPAHLSQSG
                     RALGLLGVAAACLCWGLDNNFTRKVSAADSRVIAGTKGLVAGSTNTILAIALGATLPT
                     RPYLLGSLLLGFLGYGVSLMLFIVALRHLGTARTGAYFSTSPFIGTLLAIVLYGQPVT
                     LAFYLAAVLMALGVWLHVTEHHEHEHLHEPLTHTHSHLHDAHHQHEHADEYAGAEPHT
                     HEHHHAPLRHSHAHFPDIHHQHGHHRPPG"
     gene            complement(2399..>3397)
                     /locus_tag="VNZ06_07800"
     CDS             complement(2399..>3397)
                     /locus_tag="VNZ06_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWW20693.1"
                     /translation="LATVATIIASQSIITGAYSMTRQAIRLGWLPRLRITQTSQQGYG
                     QIYVGAVNWLLALATIGLTLFFGKSDNLAAAYGIAVSLTMLMTSALLYIAMREVWGWG
                     RLASVSLAGALLCIDAAFFCANILKIFEGGYVPLLLAALVYTVMLTWHRGTAAVSRAV
                     NEKLTPIAAFLAEISARQIPRVPGTAVFITRTSRDTPPVLKWHVKKNRALHGKVFILT
                     VVTESVPWIDENARLAITELAPDFWRASARYGFMERPDIPELLRHTEPLGCALQLDDV
                     TYYVGHETIIRAQDGRGLCAWEEALYAAMVRNAAHISDLLHLPSDSVVEIGRQIAI"
BASE COUNT          599 a         1092 c         1044 g          662 t
ORIGIN      
        1 gcgcgctgcc gcgttcggcc tgttacactc cggccgcggg aattgggggc gaatatccag
       61 gcgcgagcct aggggtgttc tatagtgtcg tcgatccgcc caatcaaaga gcagaagcgg
      121 gtcttcgtcc gtcagtattg caaatcagat gacctcaagg gattgaggca ggtcctgacg
      181 acttgtgcgg cgctcgcgct cctgtggtgg ggcgcagcag cgagcataga cgcctccatc
      241 tgggtgatga tccccgtcgt cttgctgatc agcctgttca tgttgcgcgt ttttgcgctg
      301 atgcacgaat gcggccacgg cagcctgttt cacacccagc ggttaaatcg cggtttcggc
      361 tttctactgg gcgtcatgtc cggcatgccc cagtacgtgt ggtcacagca tcacaactat
      421 caccatgctc acaacggcaa ttgggacaag taccgcggtc cgtacaccac gctctcggtc
      481 gatgagtacg ctgcgctcag ccccatccgg cagcgcatgt accggtgcaa gtgcagcatt
      541 gccggggcac ccatcgcggg tttcatatat ctggtgttca acccgcgctt caattggctc
      601 aagggcagca ttgcctttat cgcacatgtc atgcgacgca agctggccga gcccgctcaa
      661 tcattcatgg ttcatgccgc gagcttcagt accccctact ggaaatcggt cagagagtac
      721 cggcacatgg cctggaacaa tatcgccctg ctcagcatct gggcgttgat gtgcagcgcc
      781 ctgggcccgt cactgttctt caagatctac ctgagcaccc tgtcgctggc cggtggtgcg
      841 ggcatcgtat tgttcacggt gcagcacaat ttccggcacg cgtatgccag cgacgaaaag
      901 aactgggact acgataccgg cgccgtcgaa ggcacgagct ttctgatcct gcccgcctgg
      961 ttgaactggt ttaccgccaa catcggctac caccacgtcc atcatctgtc tgccaggatt
     1021 ccgaactatc gcctgatcgc gtgtcacaac gaataccgcc atctgttttc cgccgtcact
     1081 cgcgtgaagc ttgcgcagat caacgaagcg ctgaaatgca tactgtggga cagccacgcg
     1141 cagcagatta tctcggtggc ggagtatcag cagcaggtga agagagcgcc gtcggcggct
     1201 tgatgctcgg agagtgacac cccgggcgcc gcgccgattg cgcgtcgcgc tgccagttga
     1261 gtgcctagat gaacgcacgc cgatcgaccc gcgcggactc cggattgcat gccggggtcg
     1321 cagccgcgct ggtatctgcg ctgcttttcg gggccaccac gccgatagca aagcagcttc
     1381 tgcagggcgc gaccccgctg ctcatcgcgg gcctcttgta cctcggcagc ggcatcgggc
     1441 tcgggcttct gcgcctgatc caggatcgcc gctggcggcc gatcggactc aggggcgcgc
     1501 aatggtcgtg gctcgccgcc gccacgctca ccggcggggt gctcgcgccg gcactgctca
     1561 tgctcgggct gactcggagc gacgccgcca ccgcctcgct gctgctgaat cttgaagccg
     1621 tgttcagcgc ggtactggcc tggttgattt tccgggaggc gaccagccgg cgcgtggtgc
     1681 tggggtttgt ttcaatcttc gtcggcgggc tgctgctggc gtggccggct catctgtctc
     1741 aatcgggccg agcacttggc ctgctcggcg tggcagcggc gtgcctgtgc tggggactgg
     1801 ataacaactt cacgcgcaag gtctcggcag cagactcgcg tgtgatcgcc ggcaccaaag
     1861 gactggtcgc cggcagtacc aatacgatcc tggcgattgc gcttggcgcc acactaccca
     1921 cgcgacctta cctgctgggc agtctgttgc tcggctttct gggttacggc gtgagtctca
     1981 tgctcttcat tgtcgcgctg cgtcatcttg ggacggcacg caccggcgcg tacttttcca
     2041 cctcaccgtt tattggcacc ctgctcgcga tagtcctcta cggacagccc gtcacgctgg
     2101 cgttctatct cgccgctgtg ttgatggcgc tcggcgtctg gctgcacgtc acggagcatc
     2161 acgagcacga acacctgcac gagccgctga cgcacacgca ctctcacctg cacgatgcgc
     2221 accaccagca tgagcacgcc gacgagtatg cgggagctga accccacacg cacgagcatc
     2281 accacgcgcc cctgcgccac agccatgcgc acttcccgga cattcaccac cagcacggcc
     2341 accatcggcc acccgggtga gcttgaggcg cgcgcgcggc tcggtgggtg ttcgcagctc
     2401 agatagcgat ctgtcgcccg atttccacca cgctgtcgct cggcaggtgg agcaagtcgc
     2461 tgatgtgcgc cgcgtttctc accattgcgg cgtagagcgc ttcctcccag gcgcacagcc
     2521 cgcggccatc ctgcgcgcgg atgatcgtct cgtggccgac atagtaggtg acatcatcca
     2581 gttgcagcgc acagccgagc ggctcggtgt gccgcaacag ctccggaata tcgggacgct
     2641 ccatgaaacc gtagcgagcg ctcgcgcgcc agaaatcggg cgccaactcg gtaatggcga
     2701 gacgcgcgtt ctcgtcgatc cacggaacgg actccgtcac gaccgtcagg atgaacacct
     2761 tgccgtgcag ggctcgattc ttcttgacgt gccatttcag gaccggcggc gtgtcgcgcg
     2821 aggttcgggt gatgaacacg gccgttcccg gaacgcgcgg aatctgccgc gcactgattt
     2881 cggcgaggaa tgccgctatt ggcgtcagct tctcattgac ggcgcgcgac acggcggcgg
     2941 tgccacggtg ccaggtcaac atcactgtgt aaaccagggc agccagcagt agcgggacgt
     3001 agccgccttc gaagatcttc agaatattgg cgcaaaagaa tgccgcatcg atgcacagca
     3061 gcgcgccggc gaggctgacg ctcgccaacc ggccccatcc ccacacttca cgcatggcga
     3121 tgtacagcag cgccgaggtc atcagcatgg tgagcgacac ggcgatgccg tacgccgccg
     3181 ccaggttgtc cgacttgccg aagaacagcg tcaggccgat ggtcgcgagg gcgagcagcc
     3241 aattgacagc gccgacatat atctgtccgt aaccttgctg ggaggtctgc gtgatccgca
     3301 gccgcggcag ccagccgagg cggatggctt gccgcgtcat cgagtaggcg ccggtgatga
     3361 tcgactggct ggcgatgatg gtcgccactg tcgcgag
//
LOCUS       DATMRM010000267         3003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2631033, whole genome shotgun sequence.
ACCESSION   DATMRM010000267 DATMRM010000000
VERSION     DATMRM010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2631033"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2371)
                     /locus_tag="VNZ06_07805"
     CDS             complement(<1..2371)
                     /locus_tag="VNZ06_07805"
                     /inference="COORDINATES: protein motif:HMM:NF021424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWW20694.1"
                     /translation="MSIHVALNHVSHYRYDRPVSLSPQLIRLRPAPHCRTRILSYSMR
                     VAPARHFVNWQQDPQANYLARLVFAERTREFRVEVDLVAETAVINPFDFFLSANAESV
                     PFQYDATESNELGPYLQCVEPTPLFTQYLASVPRSPIRTIDFLVSLNQRVAHDVRYLI
                     RMEPGVQTPEQTLQRAAGSCRDSGWLLVQLLRHLGLAARFVSGYLIQLTADIKALDGP
                     SGPPQDFTDLHAWCEVYLPGAGWVGLDPTSGLMAGEGHIPLACTPEPSSAAPITGAVD
                     ESEVQFEHAMSVTRVWEAPRVTKPYSEAQWEAIQSLGAAIDDELDQGDVRLTMGGEPT
                     FISIEDPDGAEWNTAALGPSKRRRAAELYHRLREHYAPQGLVHFGQGKWYPGEQLPRW
                     SLNCFWRRDGEAVWRDRGLIASEATDYGVTPAMAQKFARGVAERLGLDPRYVFAGFED
                     VFYYLWRERRLPVNVDPLDSRLDDPLERERLMRVFTQGLDRIAGHVLPIARDPSGLRW
                     QTGSWFLRAERCYLAPGDSPMGYRLPLDSQPWVTRGDYPFVHTPDQTQAFAPLAPATQ
                     VRGQLRDGIGDAEFRPRDIPGALARSRERAPAAHESAGWVSRTALCAQPRDGVLYIFM
                     PPLDRTEDYLELVTAVEAIASAMGLPVLLEGYEPPRDPRLNQFRITPDPGVIEANIHP
                     SHSWQELVERTTFLYEAARESRLGTEKFMLDGRHTGTGGGNHLVLGGATAHDSPFARR
                     PDVLASLLAYWHNHPSLSYLFSGLFIGPTSQAPRVDEARNDSLYELEIAL"
     gene            complement(2800..>3003)
                     /gene="groEL"
                     /locus_tag="VNZ06_07810"
     CDS             complement(2800..>3003)
                     /gene="groEL"
                     /locus_tag="VNZ06_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920153.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; many bacteria have multiple
                     copies of the groEL gene which are active under different
                     environmental conditions; the B.japonicum protein in this
                     cluster is expressed constitutively; in Rhodobacter,
                     Corynebacterium and Rhizobium this protein is essential
                     for growth; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWW20695.1"
                     /translation="YNAATGEFGDLIEAGILDPTKVTRLALQNSASVSGLLLTTEVMV
                     AEAPKEEAEHAHGPPGGMGGMDM"
BASE COUNT          581 a          950 c          935 g          537 t
ORIGIN      
        1 tgagcgcaat ttccagctca tagagtgaat cgttgcgcgc ctcatcgact ctcggagcct
       61 gtgaggttgg accgatgaaa agtccggaga acagatagga cagtgaagga tggttgtgcc
      121 aatacgccag caggctggcc agcacgtccg gacggcgggc gaacggcgag tcgtgcgcag
      181 tagcgccgcc gagtaccaga tgattgccgc caccagtccc ggtgtgacgc ccgtcgagca
      241 tgaatttttc ggtgccgagc cgcgactcgc gcgccgcttc atacagaaac gtcgtgcgtt
      301 caaccagctc ttgccaactg tgagaaggat gaatgttggc ctcgatcacg ccgggatcgg
      361 gtgtgatgcg aaattgattg agccgcggat cgcgcggtgg ctcatagccc tccaacagca
      421 caggcaatcc catggcgctg gcgatggctt ctaccgcggt cacgagttcc agatagtctt
      481 cggtgcgatc gagcggcggc atgaagatat acagcacgcc atcacgcggc tgcgcgcaca
      541 acgcggtacg ggagacccag cctgccgatt catgcgcggc cggtgctcgc tcgcgtgagc
      601 gcgccagcgc tccgggaatg tcgcgtggcc tgaactcggc gtcaccgatt ccgtcgcgta
      661 gctgcccgcg cacctgcgta gcaggcgcga gcggtgcaaa ggcttgtgtt tgatcgggcg
      721 tgtgaacaaa cggatagtcg ccgcgcgtca cccacggctg cgagtccagc ggcaggcgat
      781 agcccatggg cgaatcgccc ggtgccagat agcaccgttc ggcgcgcagg aaccacgatc
      841 cggtctgcca gcgcaggccg ctcgggtcgc gtgcgatggg cagcacatgg cccgcgatac
      901 ggtcgaggcc ttgcgtgaac actcgcatca gacgctcccg ctccagcgga tcgtccagtc
      961 tcgaatcgag cggatcgacg ttgaccggca agcgtcgctc gcgccacaga tagtagaaga
     1021 cgtcttcgaa acccgcgaat acgtagcgcg gatcaaggcc cagccgttcg gcgacaccgc
     1081 gcgcgaattt ctgcgccatc gctggcgtga cgccgtaatc ggtcgcttcc gaggcaatca
     1141 gcccgcgatc ccgccagaca gcttcgccat cgcgtcgcca gaagcagttg agcgaccagc
     1201 gcggtaactg ctcacccgga taccacttcc cctgcccgaa atggaccagt ccctgcggcg
     1261 cgtagtgctc gcgcagccgg tgatagagct cggcggcccg ccggcgcttg ctcggaccca
     1321 aggccgcggt attccattcc gcgccatccg gatcttcgat cgaaatgaac gtcggctcac
     1381 cacccatcgt cagccgtaca tcgccctgat cgagctcatc atcaatcgct gcgcccaggc
     1441 tctggatggc ttcccattgc gcctcgctat agggcttggt gacccgcggg gcttcccaca
     1501 cccgcgtgac tgacatggca tgttcgaact gcacttcgga ttcgtcgacc gcgccggtga
     1561 tcggtgccgc ggaggaaggc tcgggcgtgc aggccagtgg tatgtgcccc tcgccggcca
     1621 tcagtccgga ggttgggtcc agtcccaccc atccggcccc tggcaaatac acttcgcacc
     1681 acgcgtgcag gtcggtgaag tcctggggtg gaccggacgg gccgtccagc gccttgatgt
     1741 cggcagtgag ctgaatcaag tagcccgaga cgaagcgtgc cgcaagcccc aggtgccgca
     1801 ataactgcac cagcaaccag cccgagtcac gacacgatcc ggcggcgcgc tgcagtgtct
     1861 gctccggcgt ctgcactccg ggctccattc ggatcagata gcgcacgtcg tgcgccacgc
     1921 gctggttgag actcaccaga aaatcgatgg tgcgtatcgg gctgcgcggc acgctcgcca
     1981 gatactgggt gaatagcggc gtcggctcaa cgcactgaag ataaggacca agctcgttgg
     2041 actcggtcgc gtcgtactga aagggaaccg attcggcgtt ggcactgagg aagaaatcga
     2101 acggattgat caccgccgtc tcggctacca ggtccacctc gacccggaac tcgcgcgtgc
     2161 gctcggcaaa caccaggcgc gccaggtagt tcgcctgcgg gtcctgctgc cagttgacga
     2221 agtgcctggc aggcgcgacc cgcatcgaat acgaaaggat gcgcgtgcga cagtgcggcg
     2281 ccggtcgcag ccggatcagt tgcgggctga gcgacaccgg ccggtcgtag cggtagtgcg
     2341 aaacgtgatt gagagcgacg tggatggaca tacggaccgg caatgatgcc gcaatcgcgg
     2401 gtccgggcgc atgaatgccc ggttgcggtg cgaccggccc cgcccggccc acctacagag
     2461 cacccggttg cagataaaaa aaagccccgc gtagcgaggc tttaagatat tgcgcggaaa
     2521 cgggggggtc ttgttccgcg acttttcgga ttcaacggac tggcgtccgc ctcatcctgg
     2581 agacaactat acctaagaat tttcgtttgt aaagttaacc attcgttacc tatacattac
     2641 catttgttac tggtcggatc acaatcggaa atactgtgct gcagtgccgc ttttaccgag
     2701 aaaatcaggt aaatccgcgt caccgccgca tcagagaaaa agggccggaa gtctcctccc
     2761 ggccctttcc tgttgcagat cctaagatcc gccgggacct tacatgtcca tgccgcccat
     2821 gccgcccgga ggcccatggg cgtgttcggc ctcttctttc ggggcctctg cgaccatgac
     2881 ttcggtggtc agcagcagcc cggacactga agccgagttc tgcagcgcca gacgcgtcac
     2941 cttggtcggg tccagaatgc cggcctcgat caggtcgccg aactcgccgg tcgcagcgtt
     3001 gta
//
LOCUS       DATMRM010000268         3486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2000994, whole genome shotgun sequence.
ACCESSION   DATMRM010000268 DATMRM010000000
VERSION     DATMRM010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2000994"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VNZ06_07815"
     CDS             <1..233
                     /locus_tag="VNZ06_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20696.1"
                     /translation="NFARVDVVVAQRQEKAVLVADVPPAAITTDTAVPMLAAPRAEGL
                     IEIMLPGGASVRVDAQVDGRALRRVLGVLHER"
     gene            230..577
                     /gene="tnpB"
                     /locus_tag="VNZ06_07820"
     CDS             230..577
                     /gene="tnpB"
                     /locus_tag="VNZ06_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183759.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HWW20697.1"
                     /translation="MITPPPGVRVYLACGYTDMRKGMETLAMLVQQTLSHEPFSGAVF
                     AFRGRRGGLIKVLWHDGVGMCLFIKRLERGQFIWPMSSAGTVSLTAGQLSTLLEGCEW
                     RATARNLRPELAG"
     gene            696..905
                     /locus_tag="VNZ06_07825"
                     /pseudo
     CDS             696..905
                     /locus_tag="VNZ06_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024408.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(925..2301)
                     /locus_tag="VNZ06_07830"
     CDS             complement(925..2301)
                     /locus_tag="VNZ06_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWW20698.1"
                     /translation="MTSHHPDPHLVPPILLTWLSVFRPCFTAPVWNRILVLVTGAVLA
                     PGKRTVTRVLRVMGLADEPSFRRYHEVLSRARWDGQAVARRLLLYIIERLLPDGEVVV
                     GIDDTIERRWGARISARGIYRDPVRSSRGHFVKTSGLRWLSLMVAVPIPWAKRTWALP
                     FLTILAPSARWSEAKGKRHKTLTTWARQAILQTKRWLPNRRLVFVADSGFAALDLLAA
                     VRGHVCMITRLRLDANLFRPAPKRRPGQRGRTPLKGRALPKLSAVLKNKKTVWTSTVV
                     SQWYNAQQRRLLVATGTALWYHTGIAPVPIRWVLVRDPSGEHEPAAFLSTDLEAQPAT
                     ILGWFVSRWRVETTFQEVRAHLGVETQRQWSDLAILRTTPALLGLFSLITVWADGLVC
                     DAANALRPNVAAWYSKSEPTFSDAIAAVRRVLWAPPNFSMSRQTDETITIATGLLNRV
                     FQTLCLAA"
     gene            2395..2676
                     /locus_tag="VNZ06_07835"
     CDS             2395..2676
                     /locus_tag="VNZ06_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20699.1"
                     /translation="MTDPIIQRIESFRCRRGQLLAERRNRGYTLYRAGSDTPIARLRP
                     TGQDDRVEVLYWSLWKERWTSAGPFGRTILPIDEALQFIASEDIFWAVG"
BASE COUNT          654 a         1071 c         1119 g          642 t
ORIGIN      
        1 cgaattttgc gcgcgtcgat gtggttgtgg cgcagcggca ggagaaggct gtcctggtgg
       61 ctgacgtgcc ccctgcagcg ataacaacgg atacagccgt gccgatgctt gcagcgccgc
      121 gagccgaggg gttgattgag atcatgttgc caggcggcgc ctcggtgcgc gtcgacgcac
      181 aggtcgacgg ccgtgcattg cgccgtgtgc ttggcgtgct gcatgagcga tgatcacacc
      241 gccgcccggt gtgcgggtgt atcttgcctg tggctacacc gatatgcgca agggtatgga
      301 aacattggcc atgctggtac agcagacact gtcacatgag ccgttctctg gagcggtttt
      361 cgcattccgc ggtcgccggg gtggattgat caaagtgctg tggcacgacg gtgttggaat
      421 gtgcctgttc atcaaacgtc tggaacgcgg gcagttcatc tggccgatgt cgtctgctgg
      481 aaccgtatcg ctcacagcag gtcagctctc cacattgctc gaaggctgcg agtggcgggc
      541 cactgcgcgc aatctgcggc cggaactggc gggctgacac gcatatggtg tgatcacgca
      601 agagtcattg gcaacgacgc gacgacgtgc atcatcgtta cggtgtgcag atcgatcttg
      661 cggccctacc tgacgatccg gatgtcctgc agcggatgct gcgggagatt gtgcctgagc
      721 tgcaggccga gaacgagaag ctgttgcttc tgatccaacg tctgctgcgc catcgcttcg
      781 gcccgcgttc cgagaagctc gacctcgatc aactgcggct gatccttgag gatgaggagc
      841 aaggtgccgc ggaagacgaa gccgccgaag aggcggcaga gccgtcgaac aaacggcgac
      901 gcactcagct gggctttccg caattcaggc ggcgaggcag agagtctgga atactcgatt
      961 tagcaatccg gttgcaattg tgatggtttc atccgtctgc cgggacatgg agaaattcgg
     1021 tggcgcccac agcacgcggc ggacagcggc gatggcgtca ctgaacgttg gctctgactt
     1081 gctgtaccac gctgcgacat tcggacgcag tgcgtttgcg gcgtcgcaca ccaggccatc
     1141 ggcccacacg gtgatcagcg agaacaatcc gagcaacgcc ggggtggtgc gcaggatggc
     1201 caggtcggac cactgccgct gcgtctccac gccgagatgg gcccgcactt cctggaacgt
     1261 ggtctccacg cgccaacgcg agacgaacca gccgaggatg gtggcgggtt gcgcctcgag
     1321 atcggtgctg agaaacgcgg ctggctcatg ctcgccggat gggtcacgga ccagaaccca
     1381 gcggatcggc accggcgcaa taccggtatg gtaccatagt gcggtgccgg tggcgacgag
     1441 cagcctgcgc tgctgggcat tgtaccattg agagacgacc gtgctcgtcc agaccgtctt
     1501 cttgttcttc agcaccgcac tcagcttcgg caaggcacgc cccttcagcg gcgtgcggcc
     1561 ccgctgaccc ggccggcgtt tgggcgctgg ccggaacaga ttggcgtcca gtcgcagccg
     1621 ggtgatcatg cagacatggc cgcggacagc ggccagcagg tcgagcgccg cgaagccgct
     1681 gtcggcgacg aataccagac ggcgatttgg caaccagcgt ttggtctgca ggatcgcctg
     1741 ccgggcccac gtcgtcagcg tcttgtggcg cttgcccttg gcttcgctcc agcgcgccga
     1801 gggcgccagg atggtcagga acggcagagc ccatgtccgc ttggcccagg gtatcggtac
     1861 cgcaaccatc agcgacaacc agcgcaggcc gctggtcttg acgaaatggc ccctggagga
     1921 tcggaccggg tcacgataaa tgccgcgtgc gctgatacgc gcgccccagc gacgctcgat
     1981 ggtgtcgtcg atgccgacca ccacctcgcc gtctggcagc aagcgctcga ttatatagag
     2041 caacaaccgc cgtgcgacgg cctgcccatc ccagcgtgcc cggctcagca cctcatgata
     2101 gcgacggaag ctcggttcat ccgcgagccc catgacgcgc agcacccggg tgacggtacg
     2161 cttgcccgga gccagcaccg caccagtcac caggaccagg atgcggttcc agaccggggc
     2221 ggtgaaacag gggcggaata ccgacagcca ggtgagcagg atcggtggga ccagatgtgg
     2281 gtcgggatgg tgcgatgtca ttgcagtcgt agaatcgaga cggcacccgc gccgcaagag
     2341 tcgcccgcct ccctccgcga cgttcgccgg cgaggcgatt tatcctgcgc catcatgacc
     2401 gatccgatca ttcagcgtat cgagagcttc cggtgtcgcc gcgggcaact gctcgccgaa
     2461 cgtcgcaatc gcggctacac gctctatcgc gccggctccg acacgccgat cgcgcgactg
     2521 cgtccgaccg gacaggacga tcgtgtcgag gtgctatact ggtcgctatg gaaggagcgc
     2581 tggaccagtg ccgggccgtt cgggcggacc atcctgccca tcgacgaggc cctgcagttc
     2641 atcgcttcag aggacatctt ctgggctgtg ggataaccgg ccgggggatg caaaatgcgg
     2701 aaagcccagc tcatctatag actcccttag tccgaggtcc gctttcggct cggctgcagt
     2761 ctctactggc atcccgcgat agttattccc cgagggctct cagaatagga taccggcctg
     2821 ccgggtatga gacgctgcag cacggggtcg ggacaaggcc tagctccact tcgacagcca
     2881 ccggcgcttc gagctgcgta ggccctgcga tcccggccga catccgcgac gggctcccat
     2941 ccagattgtc gcactgcatc acccagcaaa actccgcgcg ctcggctcat agccgacgca
     3001 ttttcgtttg caacaggtcg cgaaagttga cccctgctca acaccgcacc gcgatgtcgt
     3061 cggcgtattc agccgccgct ggcatatggg tttggtggga acagccagat cccaggcgag
     3121 gccgcagtag aagtcccgaa aatctccgtt ttccccagtg tttctaaggt tgtggccaaa
     3181 tcctccttcg aaggcgcatt ggatagcctg gccgtcctgg ccgctggcgt tccccaagaa
     3241 tcaggataaa catagccgac tgtgtcgatc ggctgcgcag cgcaagccga gattaccact
     3301 gttgtggcaa cgattgcgat ggatctgcct gtcataggct cactccccag gtttgcttcg
     3361 ccagcagcga tgagcattcg taggaccagc tatcgaggca aaaaattccc ctcaatgggc
     3421 ggcattcccg agggcttcgg aatagtatgc cggccagtcg ggtatgggac gctgcagcct
     3481 gcagtc
//
LOCUS       DATMRM010000269         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3538645, whole genome shotgun sequence.
ACCESSION   DATMRM010000269 DATMRM010000000
VERSION     DATMRM010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3538645"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1392)
                     /locus_tag="VNZ06_07840"
     CDS             complement(181..1392)
                     /locus_tag="VNZ06_07840"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWW20700.1"
                     /translation="MMRGRAPIGSTVGLVLPGGGARAAYQVGVLKGIAGMFPRGARNP
                     FPIIVGTSAGAVSAAVVATEAYRWQHAVESLERVWANFHVRQVFKVDGASMLRSGMHW
                     LLSLVSGGVLLPPPLALFDNSPLRALLAQNIRWAGLRRSIENEDLEAVALCATSYSSA
                     SSVAFFQGGSRVTEWSRQQRIGLRTTLRIDHVMASLGVPFLFPPVQLGDEYFGDGAQR
                     QLWPLSPAIHLGADRLLVIGVRAEHGAGVNTRPRTVVQPPTPGQLFGYMLDTLFMDQI
                     YANVEHVQRLNSVVEVAPSAVPGMHKVATLVIAPSADLRVIATRHVRSLPRGLRSLLR
                     VLGARGSAGAQLASYLMFESSFTQELIQLGYQDAMGKREDLMGFLAGAPLAATTMLPA
                     LELADIEQTQP"
     gene            complement(1470..>2133)
                     /locus_tag="VNZ06_07845"
     CDS             complement(1470..>2133)
                     /locus_tag="VNZ06_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWW20701.1"
                     /translation="GLQKTIIRQSLARNRVVITATQMMESMIQNQVPTRAEVSDVANA
                     VLDGTDAVMLSAETAAGKFPVKAVQAMADVIRGAEKYQLGSPRSMSRAEEEFGNSEEA
                     IAMAVMYTANHMRVRAIVALTESGMTPLWMSRIRSDIPVYAFTRHEATRRRVTLYRGV
                     YAVPFDIVHTNTEALYSAIFERLLDLGLVDVNDLVILTKGELTGVQGGTNSMQILRVV
                     TR"
BASE COUNT          418 a          729 c          629 g          357 t
ORIGIN      
        1 atgacagtca cccccttacc ggcacgcccc tgtgtctggc gtgcgaccct tacctgagca
       61 ccctggggtc gttgaggctg gccggccgcg ctcttgcaca cacactgcgc actcggacgg
      121 cggcagccgg ggcagatgcg acccaccccg gtgcgataga caatactcac gcttcggcac
      181 tcaaggctga gtctgctcaa tatccgccag ttccagcgcc ggcagcatcg tggtcgccgc
      241 cagtggggcg ccagccagaa agcccatcag atcctcacgc ttgcccatcg catcctgata
      301 cccgagctgg atgagttcct gcgtgaacga ggactcaaac atcagatagc tcgcgagctg
      361 tgcgccggcg gacccgcgcg cgcccaacac ccgcagcagg gagcgcaggc cgcgcggcag
      421 gctgcgcaca tggcgcgtcg caatgacgcg caggtccgcg ctcggcgcga tcacgagcgt
      481 cgcgaccttg tgcatgccgg gcactgcgct tggcgcgact tcgaccacac tgttgagacg
      541 ctgcacgtgt tcgacgttgg catagatctg atccatgaac agcgtgtcga gcatgtaacc
      601 gaacaactgt cccggcgtcg ggggctgcac cacggtacga ggacgcgtgt tgacgcccgc
      661 tccgtgttcc gcccgcacgc cgatgaccag cagacgatcg gcgcccagat gaatagcagg
      721 actcaagggc cacagctgcc gctgcgctcc atcgccaaaa tattcatcgc ccagctgcac
      781 cggcgggaac agaaacggaa cgcccagact cgccatcacg tgatcgatcc gcagcgtcgt
      841 gcgcaggccg atgcgttgct ggcgcgacca ttccgtgacc ctgctgccgc cctggaaaaa
      901 agcgacggat gaggccgaac tgtagctggt cgcacacagc gccacagcct ccagatcctc
      961 gttctcgatg ctacggcgca ggcccgccca tcggatgttc tgcgccagca gcgcgcgtag
     1021 cggtgagttg tcgaacaatg ccaacggcgg tggcaacagc acaccgccgg aaaccagcga
     1081 cagcaaccaa tgcattccgg atcgcagcat gctcgcgcca tcgaccttga acacctgccg
     1141 cacatggaag ttcgcccaca cgcgctcgag tgattccacc gcgtgctgcc atcgataggc
     1201 ctcggtcgcc accaccgccg ccgacaccgc gccggccgag gtgccaacga tgatcggaaa
     1261 cggattgcgg gcaccgcgcg gaaacatgcc ggcgatgccc ttgaggaccc ctacctgata
     1321 ggccgcacgg gctccgccac caggcaacac caggcctacc gtcgaaccga tcggtgcgcg
     1381 gcctctcatc atgcttgcgg ccatacacgt gaatatagcg gactgcgcct gtccgaccga
     1441 tagactcgtc cagatctgct accggcgtct cagcgggtga ctacccgcag gatctgcatg
     1501 gagttggtac cgccctgcac ccccgtcaac tcacccttgg taaggatcac caggtcgttg
     1561 acgtcaacca gccccaaatc caatagacgc tcgaaaatcg cgctgtagag cgcctcggtg
     1621 ttggtgtgca cgatgtcgaa gggcaccgcg tagacgccgc ggtagagcgt gacccgccga
     1681 cgcgtggcct catggcgtgt aaacgcatac accggaatgt cggatcgaat acgcgacatc
     1741 cacagcgggg tcatgccgga ctcggtcaag gcaacgattg cacgtacgcg catgtgattg
     1801 gccgtgtaca tcacagccat cgcgatagcc tcttcgctgt taccgaattc ctcttcggcg
     1861 cgcgacatcg aacgcggcga gcccagctga tatttctccg cgccgcgaat gacgtccgcc
     1921 atggcctgta cggccttgac cggaaatttg cccgctgccg tctcggccga cagcatcacc
     1981 gcgtcggtgc cgtcgagcac ggcgttggcg acgtcgctga cttcagcacg cgtcggcacc
     2041 tggttctgaa tcatcgattc catcatctgc gtggccgtta tgaccacgcg gttgcgagcc
     2101 agtgactggc gaatgatggt cttctgaaga ccg
//
LOCUS       DATMRM010000270         4050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_773659, whole genome shotgun sequence.
ACCESSION   DATMRM010000270 DATMRM010000000
VERSION     DATMRM010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773659"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VNZ06_07850"
     CDS             complement(<1..483)
                     /locus_tag="VNZ06_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20702.1"
                     /translation="MNDVRHCLLTMIRFGPRLFWLAIGAALCATVLEGAGVITLLPLL
                     QMAQSKAPASGLFGVPIRMLTAVAGATPPLWLILAAFVAAITLRSMMLAAREYLAARL
                     RLGFANELRNRLFDAVCAADWVQIARQRRATLIHALSEDITRIDRGVLSLLLCIATSF
                     L"
     gene            complement(715..2064)
                     /gene="gorA"
                     /locus_tag="VNZ06_07855"
     CDS             complement(715..2064)
                     /gene="gorA"
                     /locus_tag="VNZ06_07855"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF004776.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HWW20703.1"
                     /translation="MASDYDLIVIGGGSGGLACAQRAAEYGARALIVESDRLGGTCVN
                     RGCVPKKLMWNAGEIAQVLHDAADYGFEVQWGAIHWDQLVKSREDYIRRLNGIYETTL
                     AKRKVDLIRGRARLRSAQSIVVNDRNLTGGVIVLANGGEAMYPDIPGARLGITSDGFF
                     EYPARPDRVAIVGGGYIAAELCGILAALGAGTSLMIRHELILRHFDRMLGEGLMEIMR
                     DDGIEIVTHAAPKSLARNAAGSLELTTVDGRTLGPFNSVIWAIGRTPVTAGLGLEALA
                     VELDAGGHVLTDQYQQTNIANLYAIGDVTPRAGLTPVAIAAGRRLSDRLFGGMSGRKL
                     DYDNIPTVVFSHPPMGTVGFSEQDARARYGDRIKVYTSSFIPLYHCLTKRKPRVRMKL
                     VTLGPIQRIIGLHVIGEGADEMLQGFAVAVRMGATKRDFDDTVAIHPTSAEELVTMR"
     gene            2149..>4050
                     /locus_tag="VNZ06_07860"
     CDS             2149..>4050
                     /locus_tag="VNZ06_07860"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HWW20704.1"
                     /translation="MSEATSDNPLLDTHSLPRFDAIRPEHVQPAISQLIEQQRRRVAQ
                     IEALPHPSFKTVVEPLEEMQHALSRVWSPVGHLNAVMNSESLRIAYNACLPLLSEYHT
                     DLAQSERLYQAYSQIARQPDASLDPVQREVIEHALRDFRLAGVALDPARKVRFKTIMM
                     ELSQLSAKFEENVLDATNTWRYHVTDAAQLSGINQSIIDQAVLRAQGKGLQGYLFGLD
                     QPTYVAVLTDAESESLRRVFYEAWSTRASGLNASAGAYDNSAVMEDILRLRHEAAQLL
                     EFKSYAEYALANRMARTVSEVTGFLNDLVRSAKVAGAHEFAELEQFAGRKLNAWDVTF
                     YSERLQQQRYCVSQEELREYLPLPRVLEGLFEVAEKLFDVRIRERRGVALWHPDVRYF
                     EVQSPDGVPVASFYLDAYARAHKRSGAWMDDCIGRKSLSGSTILPVAYLVCNSLPPSG
                     GRPAQLTHDDVVTLFHEFGHGLHHMLTRVNYPSLAGINGVAWDAVELPSQFMENYAWQ
                     SEVLTRISSHERTGGPIPADKQQQLIATRSFQAGLQTLRQVEFALFDMRLHAEYDPAQ
                     GGRVYEILREVRQRVAVVPIPEWNRYPHSFGHIFSGGYAAGYYSYKWAEVLAADAFAA
                     FSEAGAFDRT"
BASE COUNT          773 a         1287 c         1244 g          746 t
ORIGIN      
        1 caggaacgag gtcgcaatgc acagcagcag gctcagaacc ccccgatcga tgcgggtgat
       61 gtcctcggac agcgcatgaa tgagagtcgc gcggcgttgc cttgcgatct gaacccagtc
      121 ggccgcgcat accgcgtcaa ataagcggtt gcgaagctca ttggcgaatc cgagacgcaa
      181 tcgagccgcc agatactcgc gggccgcgag catcatcgag cgcagggtga tcgcggcaac
      241 gaaggcggcc agtattaacc atagcggcgg ggtagctccc gccacagccg tcagcatccg
      301 gatgggcact ccaaacagcc cgcttgctgg agcctttgac tgcgccattt gcagcagcgg
      361 cagcaacgtg atgacgcccg caccctcgag cacggtcgcg cacagcgccg cgccgatggc
      421 cagccagaag agccgcggac cgaaccgaat catcgtcagc aggcaatggc gtacgtcgtt
      481 caacatgcaa agaccatcag gcgccggcct gcgcgaaccg ccgtccgcgc tgcgcacaaa
      541 aatcccggtg tcgtcatctg ggtctgcggg tcggcgagcg cgactgcgct ccactgcggt
      601 agcggtttga cgagcctcga cagtactcgt cgtggattca ttgccagcac acgcagtaag
      661 tgcccgggtg tgcccaaatt gtttgatttg tccatttggg gctccaagac gccctcaacg
      721 catggtaacc agttcttcgg cgctggtcgg gtgaatcgcc acggtgtcgt cgaaatcgcg
      781 cttggtggcg cccatccgca cagccaccgc gaagccctgg agcatctcat cggcgccttc
      841 gccgatcaca tgcaggccta tgatccgttg gatcggtccc agcgtgacca gcttcatgcg
      901 cacccgcggc ttgcgtttgg tcaggcagtg atacagcgga atgaagctgg aggtatatac
      961 cttgatgcga tcgccatacc tggcacgggc gtcctgctcg ctgaatccga cggtacccat
     1021 cggcgggtga ctgaatacca ccgtgggaat attgtcatag tcgagcttgc gccccgacat
     1081 accgccgaac aggcgatcgg acaggcgtcg tccggcggcg atagccacag gcgtcaatcc
     1141 ggcgcgcggg gtcacatcgc cgatggcgta cagattcgcg atattggtct gctggtattg
     1201 gtcggtcagc acatgaccgc cagcgtcgag ctcgaccgcg agcgcttcga gtcccagacc
     1261 cgctgtcaca ggcgtgcggc cgatggccca gatcacactg ttgaacggcc cgagcgtgcg
     1321 cccgtcaacg gttgtcaatt ccagggatcc ggccgcattg cgtgccagtg acttcggcgc
     1381 agcatgcgtg acgatctcaa tgccatcatc gcgcatgatc tccatcagcc cttcaccgag
     1441 catccggtcg aagtgtcgca ggatcagctc gtgtcgaatc atcaggctgg taccggcgcc
     1501 gagcgccgcc agaatcccgc acagttcagc tgcgatatag ccgccgccca cgatggccac
     1561 tctgtcggga cgcgcgggat actcgaagaa tccgtccgaa gtgataccga gcctggcgcc
     1621 cggaatgtcc gggtacatgg cttcgccgcc attggccaat acgatcacgc cgcctgtgag
     1681 gttgcggtca ttgaccacga tgctttgtgc ggacctcagg cgcgcgcgtc cgcggatcag
     1741 atcgactttg cgcttggcga gggtggtctc gtagatgcca ttcaggcgcc ggatgtaatc
     1801 ctcgcgactc ttgaccagct ggtcccagtg gatagctccc cactgcacct cgaagccgta
     1861 atccgcagca tcgtgtagca cctgtgcaat ttcgccggcg ttccacatca gcttcttggg
     1921 cacgcatccc cggttgacgc aggtacctcc cagccgatcg gattcgacga tcagggcacg
     1981 agccccgtat tccgccgcgc gttgcgcgca ggccaggccg ccactgccgc caccgatcac
     2041 tatcaggtcg tagtccgatg ccatgcgccc taacctccgg aatccggcat gttacaccgc
     2101 caactcatgg ctaaaatgtg cccatacatg gccccaaggg tacgactcat gtccgaggcg
     2161 acttccgata acccactgct ggatacgcac tcactgccac gattcgacgc catccgtccg
     2221 gagcacgtgc agccggcgat ctcgcagctg atcgagcagc agcgccggcg cgtcgcgcag
     2281 atcgaggctc tgccccaccc gagcttcaag acggtggtcg agccgctgga ggaaatgcag
     2341 cacgcgctga gccgggtctg gtcgccggtc ggacacctca acgccgtgat gaatagcgag
     2401 agcctgcgca tcgcctacaa cgcgtgcctg ccgctgttgt ccgaatatca caccgatctg
     2461 gcccagagcg aacgcctcta tcaggcctat tcgcagatcg ctcggcaacc tgacgcctcg
     2521 ctcgacccgg tgcaacgtga agtgatcgaa cacgcgctgc gggattttcg cctcgcgggt
     2581 gtcgcgctgg atccggcccg taaagtgcgc ttcaagacca tcatgatgga attgtcgcag
     2641 ctgtccgcga agttcgaaga aaacgtgctc gatgcgacca acacgtggcg ctatcacgtg
     2701 accgatgccg cgcagctcag cggtatcaat cagagcatca ttgaccaggc tgtgctgcgc
     2761 gcccagggca aggggctgca ggggtatctg tttgggctcg atcagccaac ctacgtcgcg
     2821 gtcctaaccg atgccgaatc tgagtcgctg cgccgtgtat tctacgaagc ctggtcaacc
     2881 cgcgcctctg gcctcaacgc cagtgcggga gcgtatgaca acagcgccgt gatggaagac
     2941 atcctgcggc tgcgacatga ggccgcgcag ctgctggagt tcaagagcta cgccgaatac
     3001 gcgctcgcca atcgtatggc acgcacggtg agtgaagtga ccgggttcct gaacgatttg
     3061 gtgagatcgg ccaaggtggc gggtgcacac gagttcgccg agctcgagca gttcgccggt
     3121 cgcaagctca atgcctggga tgtgaccttc tattccgagc ggctgcagca gcagcgctac
     3181 tgcgtgtcgc aggaggaact gcgagaatac ctgccgctgc cacgggtgct ggaaggcctg
     3241 ttcgaggtcg ccgaaaagct gttcgacgtg cgcattcgcg agcgccgcgg cgttgcgctc
     3301 tggcacccgg atgtgcgcta cttcgaagtg caaagcccgg acggtgtgcc ggtcgccagc
     3361 ttctatctgg atgcttacgc gcgtgcgcac aagcgcagcg gcgcctggat ggatgattgc
     3421 atcggacgca agtcgctcag cggcagcaca atccttccgg tcgcctacct ggtatgcaat
     3481 tcattgccgc cttcgggtgg gcgtccggcg caactcacgc acgacgatgt ggtgacgttg
     3541 tttcatgaat tcgggcacgg cctgcatcac atgctcacgc gcgtcaacta tccgagcctc
     3601 gccggcatca acggcgtggc ctgggacgcc gtcgagctgc ccagccagtt catggagaac
     3661 tacgcctggc agagcgaggt gctgacccga atttcttcgc acgagcgcac cggcggtccg
     3721 atcccggccg acaagcagca gcagctgatc gcgacccgca gcttccaggc aggactgcag
     3781 acgctgcggc aggtggagtt tgcattgttc gatatgcgtc tacacgcgga gtacgacccg
     3841 gcacaaggcg ggcgcgtcta cgagattttg cgcgaggttc gtcagcgggt ggcggtggtg
     3901 ccaattccgg aatggaatcg ctacccgcat agtttcggcc atattttctc cggtggctac
     3961 gcggccgggt actacagcta caaatgggcc gaagtgctgg cagccgatgc gttcgccgct
     4021 ttctcggagg ccggcgcgtt cgatcggacc
//
LOCUS       DATMRM010000271        11655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1723405, whole genome shotgun sequence.
ACCESSION   DATMRM010000271 DATMRM010000000
VERSION     DATMRM010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1723405"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2203
                     /gene="alaS"
                     /locus_tag="VNZ06_07865"
     CDS             <1..2203
                     /gene="alaS"
                     /locus_tag="VNZ06_07865"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HWW20705.1"
                     /translation="DIGLAPERIVRIGDKPDGSSDNFWQMGDTGPCGPCSEIFYDHGP
                     SVAGGPPGSPEQDGDRWVEIANLVFMQFDRDVSGSLTPLPKPSVDTGMGLERMTTVMQ
                     GVHSIYEIDLFQALIQAAVRATGTSDFHSNSLKVIADHIRACSFLIVDGVVPSNEGRG
                     YVLRRIIRRAIRHGYKLGQDQPFFHKLVPTLVGLMGEAFPELSDAATLVTEALRQEEE
                     RFAQTLTSGMAQFETRMRELGGNIVPGTLVFLLHDTYGFPPDLTADIARERELQVDLA
                     GYEREMQAQRDRARAASKFGADLRAGAEVAEKTEFVGYEAVECQARVLVLMHEGARVE
                     SLSAGQQGEVILDRTVFYAEAGGQVGDSGEILGPNQTRFTVTDTQKRGSAHSHTGRVS
                     EGTVRLGDRVVARIDAERRGAIRLNHSATHLLHAALRKVLGRHVTQKGSLVAADRLRF
                     DFSHSQPMTTAEVAEVERLINAQIRANAEAATRVMEYEQAIAAGAMALFGEKYERQVR
                     VLSFGEFSTELCGGTHVKRAGDIGMFHIVSESGVAAGVRRIEAVTGQGALDYFDRVNT
                     TLSDIAQLVHGSREDVAAKVRDALERVRTLERENRGLKDKLALGQGTDLAAGAVDVAG
                     TKVLATKVSGADAGALRAAVDQLKSRLGSAVVVLGSVEADSKVLLVAGVTSNLTGRIK
                     AGELIGAVAAQVGGKGGGRADFAQAGGNQPAALDDALSSVAPWVRSRLTAD"
     gene            2322..2558
                     /gene="csrA"
                     /locus_tag="VNZ06_07870"
     CDS             2322..2558
                     /gene="csrA"
                     /locus_tag="VNZ06_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HWW20706.1"
                     /translation="MLILTRRVGETVMIGDEVAVTVLRVKGNQVRLGVNAPKSISVQR
                     EEIFQRIKREAGQAASSAESAEMAEAEAETAHTS"
     gene            2646..2736
                     /locus_tag="VNZ06_07875"
     tRNA            2646..2736
                     /locus_tag="VNZ06_07875"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2680..2682,aa:Ser,seq:gct)
     gene            2869..3381
                     /locus_tag="VNZ06_07880"
     CDS             2869..3381
                     /locus_tag="VNZ06_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWW20707.1"
                     /translation="MQLTVREMTVEEVDLIISYFHNSTPEHLETLGVDPTRLPTPQAW
                     RDRYRREYTLPITQRATLLVIWLSSDQPIGFSTSDKIVYADRAHMHLHIVHPDNRRSG
                     LGVACVRKSADIYFERLKLKSLFCEPNAFNVAPNRTLQKAGFKYIKTHMTVPGLLNYH
                     QAVTRWVIER"
     gene            complement(3338..3922)
                     /locus_tag="VNZ06_07885"
     CDS             complement(3338..3922)
                     /locus_tag="VNZ06_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWW20708.1"
                     /translation="MTPVLETRRLRLRPLQLSDADQVQALFPHWEIVRYLNAHVPWPY
                     PPDGAHNFYRDVALPGVERGERWEWTLRLKSAPEQIIGGIGLMLGESENRGFWTGLPW
                     QGQGLMSEACEAVTHFWFEVLKFPRLRAPKAVANTASARISRRQGMRIIGREERDLVC
                     GRVPAEIWEITAEEWRGYCRLSVRSPSASQPDGS"
     gene            complement(3919..4101)
                     /locus_tag="VNZ06_07890"
     CDS             complement(3919..4101)
                     /locus_tag="VNZ06_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20709.1"
                     /translation="MWSLSFENGQTHVRYDWTVEATKAWMRVLAPLAKPMFAWNHGVV
                     MDWGYEGLQRRLGLAR"
     gene            complement(4143..5816)
                     /locus_tag="VNZ06_07895"
     CDS             complement(4143..5816)
                     /locus_tag="VNZ06_07895"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006586706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HWW20710.1"
                     /translation="MPSNIEIAQQAKLQPITAVAAKLGIPGQSLESYGHYKAKIGFEF
                     LESLKARRDGKLILVTAISPTPAGEGKSTTTVGLGDALNRIGKKTMICLREPSIGPVF
                     GIKGGATGGGYAQVVPMEEINLQFTGDFSAIALANNLLAAMLDNHIHHGNELGIDVRR
                     VTWRRVVDMNDRALREITIALGGPANGYPRQDGFDIVVASEVMAILCLATSLADLKTR
                     LGNIVVGYTRAGQPVCAGELKAHGAMAVLLRDALKPNLVQTLENNPAFVHGGPFANIA
                     HGCNSVLATGTALKLADYVVTEAGFGADLGAEKFIDIKCRKAGLTPSAAVIVATVRAL
                     KFHGGCDLKELNSENLAALERGLTNLEKHVDNVRTHYGLPCIISINHFTADTDAEVAL
                     LQSKMARHGAPVVLAKHWAQGSAGAEPLAHEVVRMTDQTAPARKFVYEDALPLWEKMR
                     TVARKIYGAADISAERSVRAQIDKLQESGYGHFPVCVAKTQYSFSTDPSLRGAPTGHI
                     VNVREVRLAAGAEFIVMICGDVMTMPGLPAVPSAVKIDLDDKGNIVGMF"
     gene            complement(5833..6690)
                     /locus_tag="VNZ06_07900"
     CDS             complement(5833..6690)
                     /locus_tag="VNZ06_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20711.1"
                     /translation="MTKLLHGLAAVLLLAGVSAAWARPRLDSPDPYHNQAVAYAAVVD
                     GEMIWGKNVDQQRAPASLTKLLTALVLLHSDWDPNAWLDVSHDAAHATHPRAGLRTGD
                     QVRADDALQAMLMHSANDACLVLAENAAGSIHAFAVRMNAMAAQLGMKNSHFVHPCGF
                     DQDGQYSTVNDLLRLAKAAHANPRIAQIVLQEEAEIGLASGRKLSFHNTNLLLGRKLG
                     VMGLKTGYTAQAGHCLIAVAEQSGHRVWLVLLDSQRRWTTANRILTDAFTVAQHGSPL
                     LQNAAYVGH"
     gene            complement(6755..7873)
                     /locus_tag="VNZ06_07905"
     CDS             complement(6755..7873)
                     /locus_tag="VNZ06_07905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HWW20712.1"
                     /translation="MLEQKLMHTEAQTQDPTLAPVAAVLPSFSLDSLIARGLVPDWLI
                     RLGIRRLLRERLREEMRGSAEDRVRRHLEFVEELKKSPIAINADAANEQHYEVPTAFY
                     QQCLGKNLKYSSGLWSAGVTTLDGAEEAMLRLTCERAGLADGQQILELGCGWGSLSMW
                     MAKQYPNARITGVSNSSTQRAFIEAECARRGLTNLTIITCDMNRFDTTARFDRVVSVE
                     MFEHMKNYQLLMGNVARWLKPQGKLFLHVFSHREVSYHFVARDASDWMARYFFTGGLM
                     PSDDLLPSFQRDLKLQSRWRVSGVHYARTAQAWLDNLDRNRAHVMPLLAETYGADQAR
                     KWLSYWRVFFMACAELWAFRGGDEWMVSHYLFGKPSVM"
     gene            complement(7845..8675)
                     /locus_tag="VNZ06_07910"
     CDS             complement(7845..8675)
                     /locus_tag="VNZ06_07910"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HWW20713.1"
                     /translation="MALSSCLYECSVVHRRLRPKRHAFEHRIMMMYADLDELPQIHRD
                     LRLLSHNRSNLYSLRESDYFPSGAPGDLKARVLSYLREHGIHLPAGGKIRLLTLPRTL
                     GYIFNPISIYFCCHTADLPVCAIAEVGNTFGEKKLFLLPLRPDARHPARFRVRVPKQY
                     YVSPFSDLDISFDFDCLLPQERLQVHIEDWDQHGKLLISHLRGRQVPLTDYQLARYAI
                     KFPALTLKVITLIHWHALRLALKRVPWHRKAKDPQLQRDVLNPTPSLLTDAGTETHAH
                     "
     gene            complement(8666..9934)
                     /locus_tag="VNZ06_07915"
     CDS             complement(8666..9934)
                     /locus_tag="VNZ06_07915"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWW20714.1"
                     /translation="MDRIAIIGTGISGLGCAYFLKHRYQVTMYDKADYIGGHTNTLDI
                     DEEGRHVPIDTGFMTYNNVTYPNLTRLFRELGVVSRDSSMSFSVQHLPANLEFSGSSI
                     NHLFGQRRNLLKPEFWKMLLQINRFNKEATAAVRSQQEHSLSLGEFVAERRYGNHFLN
                     YYLLPMSCAVWSSPPDRMLQFPAMTLLRFFHNHGFLGLHTQHPWLTVAGGAKNYVDKI
                     VAPLRDRIYLNRGAVAVRREVGGGVRVTDESGHAERYQHVILACHADQALGMLEDADA
                     HERAALGEFKYQPNWALVHTDESVMPRTQLCWSSWNYRVNYRANNQFEPSTVYWINHL
                     QGVQSKRNYFVSINGEDSMKPESVLRRIQYEHPLFNDGAVRAQRELPHLNERRTGVYF
                     CGSYFRYGFHEDAFSSALALSRLLAPETWH"
     gene            10181..10768
                     /locus_tag="VNZ06_07920"
     CDS             10181..10768
                     /locus_tag="VNZ06_07920"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWW20715.1"
                     /translation="MTADTNKNVADQTQDAALIERIRNADRTAFMTFYDRFSPLLFSV
                     AARVLGDRKEAEDVLQEVMLVIWNKSGEYHPELGSLSSWAVALTRNKALDRLRSRTRR
                     LRLIEEVAIMAEDSDGSSSPSANEVLHGRERAEMLRTAMKQLPAEQRMAIELAFFTGL
                     SQTDIATRLQQPLGTVKARIRRGMLRLREQLGGVL"
     gene            10765..11574
                     /locus_tag="VNZ06_07925"
     CDS             10765..11574
                     /locus_tag="VNZ06_07925"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWW20716.1"
                     /translation="MIDERLQDQASLYALGALDAEETREFEVALQGSPELRALVRDLR
                     AVTEVIAGTAPALEPPASVKAKLMLQIGEQRRPATVTSLNPRRRPLSVWIPWALAAGL
                     AVVSFGFLYQSAGLKAELAAQARRVDELGMSLEVAQTESGRLRQTVAELRENNRLAAI
                     RIALLDSMVAADPKAVAVSVWDNERQNGVFIVRNLKSLPSDKDYQLWIIDPKYKSPVD
                     AGVFQVDAKGNVRAEFRAHEQIQTASQFAVTIEKKGGAQTPNLKAMVLAGA"
BASE COUNT         2246 a         3461 c         3736 g         2212 t
ORIGIN      
        1 ggacatcggg ctcgcacccg agcgcatcgt gcgtatcggc gataagcccg acggcagttc
       61 tgacaatttc tggcagatgg gggataccgg tccctgcggt ccgtgcagcg agatcttcta
      121 tgaccatggg ccatcggtcg cgggcggccc acctggttcg ccggaacagg atggcgatcg
      181 ctgggtcgag attgccaatc tggtgttcat gcaatttgat cgggacgtca gcggcagcct
      241 gacgccactg cccaagccat cggtggatac gggcatgggc ctggagcgca tgaccaccgt
      301 gatgcagggc gtgcactcca tttacgagat tgatctgttt caggcattga ttcaggccgc
      361 tgtgcgcgcc accggcacat ctgatttcca ctccaattca ttgaaggtga tcgctgacca
      421 cattcgcgcc tgcagcttcc tgatcgtcga cggcgtcgtg ccttccaacg agggaagggg
      481 ttacgtgctc cggcgcatca tccggcgcgc gatccgccac ggctacaagc tcggtcagga
      541 tcagccgttc tttcacaagc tggtcccgac gctggtcgga ctcatgggag aagctttccc
      601 ggagctttca gatgcagcga cgctcgtgac cgaggcgctg cgacaggagg aggaacggtt
      661 cgcgcagacg ctaacgtcgg gtatggcgca gttcgaaaca cgcatgcgcg aactgggcgg
      721 gaatatcgtg cccggtacgc tggtcttcct actacatgac acgtatggat ttccacccga
      781 cctgaccgcg gacatcgcac gcgagcggga gctacaagtc gatctggcag gttatgagcg
      841 cgaaatgcag gcacagcgcg accgggcccg cgccgcgagc aaattcggtg cggatctgcg
      901 cgccggcgcc gaagttgccg agaaaacgga attcgtgggt tatgaggccg tggagtgtca
      961 ggcgcgtgtg ctggtcctga tgcacgaagg cgctcgcgtc gagtcgctat cggcaggtca
     1021 gcagggcgaa gtgattctgg atcgaacggt gttttatgct gaggccggcg ggcaggtggg
     1081 tgattccgga gagatcctcg gccccaatca aactcgtttt acagtaacgg acacccaaaa
     1141 gcgcggttca gcgcactcac acacaggccg ggtgagcgaa gggactgtgc gactgggcga
     1201 tagggtcgta gcgcgaatcg atgccgagcg gcgtggagcc attcgattga atcattcggc
     1261 gacgcatctg ttgcacgccg cccttcgtaa agtacttgga aggcacgtga cgcagaaggg
     1321 ttcgttggtc gctgccgacc ggctgcgatt tgacttctcg cactctcagc ccatgaccac
     1381 ggcggaggtg gcagaggtcg agcgcctgat caatgcgcag attcgggcca atgcggaggc
     1441 tgctacgcgg gtaatggaat acgagcaggc gattgcggcc ggtgccatgg cactttttgg
     1501 tgagaagtac gaacggcagg tacgtgtgct gtcgttcgga gagttctcca ctgaactttg
     1561 tggtggcacg catgtgaagc gcgcgggtga tattggaatg tttcacatcg taagcgagtc
     1621 gggtgtcgct gctggcgtgc gccgtattga ggcggtgacc gggcagggcg cactggatta
     1681 cttcgatcgt gtcaatacga cgctgtccga tattgcccag ttggtgcacg gctcacggga
     1741 ggatgtcgcg gcgaaagttc gcgatgcact ggagcgcgtt cgaacgcttg agcgtgagaa
     1801 tcgaggtctg aaggacaagc tcgcgctcgg tcagggaaca gatctggctg caggcgcggt
     1861 agatgtcgct ggcaccaaag tactggccac caaagtgtcc ggtgcggatg cgggcgcgct
     1921 gcgcgcggcg gttgatcagt tgaagagtcg tctcggttcg gctgtcgtcg tgctcggatc
     1981 ggtggaagca gattctaaag ttctgttggt ggcgggcgtt acgtcgaact taacgggacg
     2041 aattaaagca ggggaactaa ttggtgcagt ggcagcccaa gtgggcggca aaggcggcgg
     2101 tcgagccgac ttcgcgcagg ctggaggcaa tcaaccggcc gcactcgatg acgcactcag
     2161 cagtgtcgca ccttgggtcc gctcccgtct gacagcagat tgacgaattt taacgtgcgc
     2221 tttgagcgat ggcgattaag actgtttgta ctagaaattg tttgactaag gtcggttagg
     2281 cgccaaattg agctgcgcga tgaccacgga ggatctctta agtgcttatt cttacgcgta
     2341 gagtcggcga gaccgtaatg atcggtgatg aagtcgccgt gactgtgctg cgtgtcaaag
     2401 gcaaccaggt caggttgggc gtcaacgcgc caaaatccat ctctgtgcag cgcgaggaaa
     2461 tttttcaacg gatcaaacgg gaggcgggac aggccgcctc gtctgccgaa agcgcagaaa
     2521 tggcggaagc cgaggctgag accgcccaca cgagctgagc ttcaggcggt ccgggcccgt
     2581 ttttgccctg gcgacacccc ttaagtatga tggcggccgc tcgagcggtg gaccgctcgc
     2641 gtgccggaga gatggccgag tggtcgaagg cgcacccctg ctaagggtgt aagggtcaaa
     2701 agcccttcga gggttcgaat ccctctctct ccgccagaat tttacgaacg cagcggcgca
     2761 tcagagggtc tgtatgcgcc agcgcggact gaccatgccc gttcctaaca ccagcgcgag
     2821 ggccggtgct gagcctcgat tcgcaagcat gaataacgga gccgccgcat gcagttgaca
     2881 gtgagggaga tgacggttga ggaggtagat ctcatcatca gctattttca taactccacg
     2941 cccgagcacc tggaaacact tggcgtcgat ccaacgcgtc tgccgactcc gcaggcctgg
     3001 cgcgatcgct atcgccgcga atacacgctg ccgatcaccc agcgcgcgac gttgctcgtg
     3061 atctggctgt cgagcgatca accgatcggc ttctccacct cggacaagat tgtctacgca
     3121 gatcgagccc acatgcattt gcacatcgtc catccagaca accggcgcag cggcctaggg
     3181 gtcgcgtgtg ttcggaagag cgccgacatc tatttcgagc gacttaagct aaagagtctc
     3241 ttctgcgagc cgaacgcttt caacgtcgct ccgaaccgaa cacttcagaa ggccggcttc
     3301 aagtacatca aaacacatat gacggtcccc gggctgctca actaccatca ggctgtgacg
     3361 cgctgggtga tcgaacgcta agtctgcagt atccgcgcca ctcctcggca gtgatctccc
     3421 agatctccgc gggcacgcga ccacagacca gatcgcgttc ctcgcggccg atgatacgca
     3481 tgccttgtcg gcgcgagatc cgtgccgagg ccgtattggc cacggctttc ggtgcacgca
     3541 aacgcgggaa cttcaatacc tcgaaccaga aatgggtcac cgcctcgcac gcctcgctca
     3601 tcaggccctg cccctgccag ggcagcccgg tccaaaaacc gcggttttca gactcgccga
     3661 gcatgagtcc gattccgccg atgatctgct cgggcgcgct tttcaggcgc aacgtccact
     3721 cccagcgttc accgcgttcc acgcctggta gcgcgacgtc gcgatagaag ttgtgcgcgc
     3781 cgtccggcgg gtagggccat ggcacgtgcg cgttcaggta acgcacgatc tcccagtgcg
     3841 ggaaaagcgc ctgcacctga tcggcatccg atagctgtaa cggacgcagc cgcaggcgcc
     3901 gggtttccag gaccggtgtc atcgcgcgag gcccaatcgg cgttgcaagc cctcgtaccc
     3961 ccagtccatg accactccgt ggttccaggc gaacatcggc tttgccagcg gcgccagcac
     4021 gcgcatccag gccttggtgg cctcgaccgt ccagtcatag cgcacgtggg tctggccatt
     4081 ctcgaagctt agggaccaca ctcctcggcc ctgcagttcg ccgtccgcga tccacgccgg
     4141 cgctagaaca tgccgacgat attgcctttg tcgtccagat cgattttgac cgcggacggc
     4201 acggcgggca ggcccggcat cgtcatcaca tcgccgcaga tcatgacgat gaactccgcg
     4261 cccgcggcga ggcgaacctc gcgcacattg acgatgtggc cggtgggcgc accacgcagc
     4321 gacgggtcgg ttgagaagga gtactgagtc ttggccacgc acaccggaaa gtggccgtag
     4381 cctgattcct gcagcttgtc gatctgcgcc cgtacgctgc gttccgcgct gatatcggcc
     4441 gcaccgtaga tcttgcgggc cacggttcgc atcttttccc atagcggcag cgcatcctcg
     4501 tagacgaact tgcgcgcggg ggctgtctgg tcggtcatgc gcacgacttc gtgcgccagc
     4561 ggctcggcac cggcactgcc ttgcgcccag tgctttgcca ggacgaccgg tgcgccatga
     4621 cgggccatct tcgattgcag cagagcgacc tcggcatcgg tgtcggcggt gaaatgattg
     4681 atcgagatga tgcacggtaa cccatagtgg gtacggacat tgtccacatg cttctcgagg
     4741 ttggtgaggc cgcgctcgag cgccgccagg ttctcgctat tgagctcctt cagatcgcag
     4801 ccaccgtgga atttcagcgc tcgcaccgtc gcaacgatca ctgccgccga gggcgtcagg
     4861 cccgccttac gacacttgat atcgatgaac ttctcggcgc ccagatccgc tccgaatccg
     4921 gcttcggtca ccacatagtc ggccagcttg agcgcggtgc cggtcgcgag cacggaattg
     4981 cagccgtgtg cgatgttggc gaacgggccg ccgtgcacga aggcgggatt gttctccagg
     5041 gtctgtacga gattgggctt gagcgcatcg cgcagcagca cggccatcgc accgtgggct
     5101 ttcagctcgc cagcgcagac gggctgtccg gcacgggtat agccgacgac gatgttgccg
     5161 aggcgcgtct tgaggtcagc gaggctggtc gccagacaca ggatcgccat gacttcggag
     5221 gccacgacaa tgtcgaaacc gtcctggcgc ggatagccat tggcaggtcc gcccagggca
     5281 atcgtgattt cgcgcagcgc gcggtcattc atgtccacga cgcggcgcca ggtgacccgc
     5341 cggacatcga tcccgagctc gttgccgtga tgaatatgat tgtcgagcat cgccgccagc
     5401 aggttgttgg ccaatgcgat ggcgctgaaa tctccggtga actgcaggtt gatctcttcc
     5461 atcggcacga cctgggcgta gccgccaccg gtcgcgccac ccttgatgcc gaacaccggc
     5521 ccaatgcttg gctcgcgcag gcaaatcatg gtcttcttgc cgatgcgatt gagcgcgtcg
     5581 cccaggccca ccgtggtcgt ggacttgcct tcacccgcgg gggtcgggct gatcgcggtg
     5641 accaggatca gtttgccgtc gcgcctagcc ttcaggctct ccaggaactc aaagccgatc
     5701 ttggccttgt agtggccgta gctttccaga ctctggcccg gaatgcccag cttggcggcg
     5761 accgcggtga tcggttgcag cttggcctgc tgcgcaatct cgatgttgct cggcacttag
     5821 gcgcaggtcc cgtcaatgtc caacgtacgc tgcgttctgc agcagcgggc tgccgtgctg
     5881 cgcgacggtg aatgcatcgg tgaggatgcg gttggcagtg gtccaccggc gctgcgaatc
     5941 gagcagcacc agccagacgc ggtgcccgga ctgttcggcg acggcaatca ggcagtgtcc
     6001 ggcctgtgcg gtatagccgg tcttcagtcc catcacgccg agcttgcgtc cgagcagcag
     6061 attggtgttg tgaaacgaca gcttgcggcc ggatgccagg ccgatctcgg cttcctcctg
     6121 caacacgatc tgcgcaatgc ggggattggc atgcgcggcc ttggccaggc gcagcaggtc
     6181 gttgacggtg gaatactgac cgtcctggtc gaaaccacaa gggtgcacaa agtgagagtt
     6241 cttcatcccg agctgtgccg ccatcgcatt catgcgaacg gcgaaggcgt ggatgctacc
     6301 cgcggcgttc tcggccagca ccaggcaggc atcgttggcc gaatgcatca acatggcttg
     6361 caacgcatcg tcggcccgca cctgatcgcc ggtacgcagt cccgcccgcg gatgagtcgc
     6421 gtgggcggca tcgtggctga cgtcgagcca tgcgttcgga tcccagtcgg agtgcagcag
     6481 caccagggcg gtcagcagtt tggtgagcga ggcgggcgcg cgctgctggt ccacgttctt
     6541 gccccagatc atctccccgt ccacgaccgc ggcataagcg accgcctggt tgtgataggg
     6601 atcggggctg tcgaggcgcg gtctggccca cgccgcggat acgccagcca gtaacagcac
     6661 ggctgccagc ccgtgcagca gtttggtcat gtctactccc gtctccggtg gccgcaagtc
     6721 tagctgcgac cacgcggcaa cggtagtcac tagatcacat cacagacggc ttgccgaaca
     6781 gatagtgcga caccatccac tcgtcaccgc cgcggaacgc ccacagctcc gcgcaagcca
     6841 tgaagaacac gcgccagtag gacagccatt tcctggcctg atcggcgcca taggtctcgg
     6901 ccagcagcgg catgacgtga gcgcggttgc ggtcgaggtt gtccagccag gcttgggcag
     6961 tccgcgcgta gtgcacaccg gatacgcgcc agcgggattg cagcttcagg tcgcgctgga
     7021 acgagggcag tagatcgtcg ctcggcatca gtccgccagt gaagaagtag cgagccatcc
     7081 agtctgaggc gtcgcgtgcc acgaagtgat aggacacctc ccggtggctg aaaacatgca
     7141 ggaacagctt gccctgcggc ttcagccagc gagccacgtt gcccatcagc aactggtagt
     7201 tcttcatgtg ctcgaacatt tccaccgata ccacccgatc gaagcgcgcc gtggtgtcga
     7261 agcggttcat gtcgcaggta atgatcgtca ggttggtcag gccgcgacgg gcgcattcgg
     7321 cctcgatgaa tgcacgttgc gtggacgagt tggatacgcc cgtgatgcgc gcattcgggt
     7381 actgcttcgc catccacatc gacagcgagc cccagccgca acccagttcc aggatctgct
     7441 ggccgtcggc cagcccggcg cgctcacagg tcaaacgcag catcgcctcc tcggcgccgt
     7501 cgagcgtggt gacacccgcg ctccacaggc cgctgctgta cttgaggttc ttgccgaggc
     7561 actgctggta gaaggcggtc ggcacctcgt agtgctgctc gttggccgca tcggcattga
     7621 tggcaatcgg gctcttcttg agctcctcga cgaactcgag gtggcgacgg acgcgatcct
     7681 ccgcgctgcc gcgcatctct tcgcgcaagc gctcgcgcag caagcggcgg atgccgagcc
     7741 ggatcagcca gtccgggacc aggccgcgcg caatcagcga atcgaggctg aacgagggca
     7801 ggaccgctgc aacgggtgcc agggtgggat cttgcgtctg agcttcagtg tgcatgagtt
     7861 tctgttccag catcggtcaa gagtgagggg gtgggattga gtacgtcgcg ctgcaactgc
     7921 gggtccttcg ccttgcgatg ccagggcacg cgtttgagcg ccaggcgcag cgcatgccaa
     7981 tgaatgagag tgatcacttt cagggtcagt gccgggaact tgatcgcgta gcgcgcgagc
     8041 tgataatcgg tcagcggtac ctggcggccg cgcagatgac tgatgagcag cttgccgtgc
     8101 tggtcccagt cttcgatgtg cacctgcagg cgttcttgcg gtagtagaca gtcgaaatca
     8161 aaactgatgt cgagatcgga gaacggcgag acgtaatact gcttcggtac gcgcacgcga
     8221 aagcgcgcgg gatgacgagc gtccggccgc agaggcaaca gaaacagctt cttctcgccg
     8281 aaggtgttgc cgacctccgc aatcgcgcac accggcagat cggcggtatg gcagcagaaa
     8341 tagatcgaga tcggattgaa tatgtaacca agcgtgcgcg gcagtgtcag cagacgtatc
     8401 ttgccgccag ccggcagatg aatgccgtgt tcgcgcagat aactcaacac acgcgccttc
     8461 agatcgcccg gtgccccgct cggaaagtaa tccgactcac gcaggctata gagattgctc
     8521 cgattgtggc tgagcaggcg caggtcgcgg tgaatctgcg gcagctcgtc cagatcggca
     8581 tacatcatca tgatgcggtg ctcgaacgca tggcgtttag gccgcaggcg ccggtgcacc
     8641 acgctgcact cgtacaggca ggaactcagt gccatgtttc cggcgccagc agtcggctca
     8701 atgcgagcgc cgagctgaac gcgtcctcgt gaaagccata acggaagtag ctgccgcaga
     8761 aatacacacc ggtgcgacgt tcattcaggt gcggcagctc gcgctgcgcg cgcaccgcgc
     8821 cgtcgttgaa cagcgggtgc tcgtactgaa tgcggcgcag tacgctttcc ggtttcatcg
     8881 agtcttcgcc gttaatggag acgaaatagt tgcgtttcga ctggacgccc tgaaggtgat
     8941 tgatccagta gacagtggat ggctcgaatt gattgttggc gcggtagttt acgcggtagt
     9001 tccaggacga ccagcacaac tgagtgcgcg gcatgaccga ttcgtcggta tggaccagcg
     9061 cccagttggg ttggtacttg aattcgccca gcgcggcgcg ttcgtgagcg tcagcgtcct
     9121 ccagcatgcc cagcgcctga tcggcgtggc aggccaggat gacgtgctgg tagcgttcgg
     9181 cgtgaccgga ttcatcggtc actcgcacac cgccgcccac ctcacggcgc acggcgaccg
     9241 cgccacgatt cagatagatg cgatcgcgca acggggcgac gatcttgtcc acatagttct
     9301 tcgcaccgcc ggcgacggtc agccatgggt gctgggtatg caggcccagg aagccgtgat
     9361 tatggaagaa acgcaacagc gtcatcgccg ggaactgcaa catgcgatcc gggggggagg
     9421 accacaccgc gcagctcatg ggcagcaggt agtaattcag gaagtgattg ccatagcgac
     9481 gctcggcgac gaactcgccc aggctcagcg agtgctcctg ctgggagcgc accgcggccg
     9541 tggcttcctt attgaagcgg ttgatctgca gcagcatttt ccagaattcg ggtttgagca
     9601 gattacgtcg ctgaccgaac agatgattaa tgctcgaacc agagaattcg aggttcgccg
     9661 gcaggtgctg cacgctgaag gacatcgaac tgtcgcgcga gacgacaccc agttcacgaa
     9721 acagccgggt cagattcggg tacgtgacat tgttgtatgt catgaacccg gtatcgatcg
     9781 gaacgtggcg gccttcctcg tcgatatcga gggtattggt atgaccgccg atatagtcgg
     9841 ctttgtcata catcgtgacc tggtagcggt gcttcagaaa gtaggcacag cctagcccgg
     9901 agatcccggt gccaatgatc gcaatcctat ccatgggaat gtcgccgtcg ggcgccctgc
     9961 ctgtgaccct atcgtcgcat ataacgaatc ccaggtgggc gtcggatgca cctgctgaaa
    10021 gccatcgaaa gcatccgtcc gctccaaccg agcgttaatt gggtgatggt ttgaagcttg
    10081 tgagagtaat gccggtctgc tatgaatgaa tgcgcccact taccaaggaa cgtttgcatt
    10141 ggctccgccg cagtagcagc ggcctgccca atcgctaacc atgacagcgg acacaaacaa
    10201 gaacgtagcg gatcagactc aggatgcagc gctgatcgag cggatccgga acgcagaccg
    10261 cacggcgttc atgacctttt acgaccgttt ttcgccgctg ctgttctcag tggcggctcg
    10321 agtgctcggt gaccgcaagg aagccgagga cgtattgcag gaagtcatgc tcgtaatttg
    10381 gaacaaatcg ggagagtacc atccggagct gggcagtctg tccagctggg cggtcgcgct
    10441 cacccgcaac aaggcgctgg accgcctgcg ctcgcgcacc cggcggctgc gcctgatcga
    10501 agaagtggcc atcatggccg aggattcgga cggctcctcc agcccttcgg cgaatgaggt
    10561 gctccacggt cgcgagcgcg cggaaatgct gcgtaccgcc atgaagcagc tgccggccga
    10621 acagcggatg gccatcgagc tggcattttt caccgggttg agtcagaccg acatcgcgac
    10681 ccgtctgcaa cagccgctgg gcaccgtgaa ggcacggatc cgccgcggca tgcttcgtct
    10741 gcgtgagcag ctcggaggtg tgctgtgatt gacgagcggc tacaggatca ggcttccctt
    10801 tacgccctgg gagcgctcga tgccgaggag acgcgcgagt tcgaggtggc tctgcagggc
    10861 agcccggagc tgcgtgcgct ggtacgcgat ctgcgcgcgg tcaccgaagt gatcgccggc
    10921 acggctccgg cgctcgagcc tccggccagt gtcaaggcga agctgatgct gcagatcggc
    10981 gagcagcgtc gtccggcgac ggtgacgagc ctcaacccgc ggcgccgacc gctcagcgtc
    11041 tggatcccgt gggcactggc ggcgggtctg gccgtggtgt cgttcggatt cctctatcaa
    11101 tccgcgggtc tgaaggccga gctggcggct caggcgcgcc gcgtcgatga actcgggatg
    11161 agcctcgagg tcgctcagac cgagagtggc cggttgcgcc agaccgtggc cgagctgcgt
    11221 gagaacaatc gcctggcggc gatccgcatt gcgctgctgg actcgatggt cgcggcggat
    11281 ccgaaggcgg tcgcggtcag cgtgtgggat aacgagcgac aaaacggtgt gttcatcgta
    11341 cgcaatctga agtccctgcc tagcgacaag gattatcagc tctggatcat cgatccgaag
    11401 tacaagagcc cggtcgatgc gggcgtcttc caggtcgatg ccaagggcaa cgtgcgtgca
    11461 gaattccgtg cacatgaaca gattcagacg gccagtcagt ttgccgtcac gatcgagaag
    11521 aagggcggcg cgcagactcc caacctcaag gccatggtgc tcgccggagc ctaagagcgc
    11581 taagatcact ccccatcacg cgaaagcggg agggagcgac cgtgcaattc tggatgcgcg
    11641 cagccaaccg cgttg
//
LOCUS       DATMRM010000272         3097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2002551, whole genome shotgun sequence.
ACCESSION   DATMRM010000272 DATMRM010000000
VERSION     DATMRM010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2002551"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VNZ06_07930"
     CDS             complement(<1..216)
                     /locus_tag="VNZ06_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20717.1"
                     /translation="MQIRWSPRRSVGALIALCVLACALCVSTYRVFGNFWDEPEHIAA
                     GLVLVDRGQYLYDNQHPPFARLLAAIGP"
     gene            354..2870
                     /gene="thrA"
                     /locus_tag="VNZ06_07935"
     CDS             354..2870
                     /gene="thrA"
                     /locus_tag="VNZ06_07935"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HWW20718.1"
                     /translation="MTINDPKALPTPASWVVHKFGGSSVADAQCFERVAKIVESQPAQ
                     QLAIVLSACRGITDALLELVSLAEAQQPAWHERLAAVRERHAGIATTLLNAEAAAGYM
                     SQFDRDMEDVEGVLTTTSIMRSAAQTVRDLCSGYGEIWSTRLFVRYLQQRGARGGAQW
                     IDARRCVTVEWGPLGPAVQWQSSQLKLQQLLAPQGSQPAASTLVITGFIASDPRGVQT
                     TLGRNGSDFSASIFGALLEAAEIQIWTDVDGVLSADPRRVPDAQVIDSLSYNEAMELA
                     YFGAKVIHPQTMAPAVGRDIPIWIRNTFAPEKAGTLICARPTSTLPVKGITSIEGVAL
                     INLEGAGMIGVPGTAHRLFGALREESISVILISQGSSEHSICCAIPQTQAERAKNVLE
                     AAFARELTEGQIQSVDITQDQAILAVVGDGMTGMPGVSGKLFNALGNTHINVRAIAQG
                     ASERNISVVVDGKDATRALRATHASFYLSPHTLSIGVIGPGTVGQVLLDQLASQRERL
                     AREFKIDLRVRGVLRSRQMRLTDVGLQLPHWRAELLSGSQPADLARFVEHVRVDYLPH
                     TVLIDCSADAEVASHYHEWLAAGIHIVTPNKKANSGSLAYYESLKAARRASGAHYLYE
                     TTVGAGLPVIQTLRDLRETGDEITSIEGIFSGTLAYLFNVYDGTQPFSSIVREAKQRG
                     YTEPDPRDDLSGTDVARKLIILGREMGLKLELRDVQVASLVPSDLVSGSSEDFLSGLP
                     RHDAVMKRQYEGARSRDRVLRYIGALSANGTATVGLVELDERHPFANIALTDNVVRYQ
                     TARYCNNPLIVQGPGAGPEVTAGGVFADLLRLATYLGARL"
     gene            2867..>3097
                     /locus_tag="VNZ06_07940"
     CDS             2867..>3097
                     /locus_tag="VNZ06_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20719.1"
                     /translation="MTRAQATAFAPASVGNVSIGFDILGFSVDALGDCATVRRTDSPG
                     VTIRAIRGVVQDLPMEAAKNTAGRAIIAMHSAL"
BASE COUNT          537 a          984 c         1038 g          538 t
ORIGIN      
        1 gggaccgatc gcagccagca ggcgcgcgaa cggcggatgc tggttgtcgt acaggtactg
       61 accgcgatcg accagcacca ggccggccgc gatatgctcc ggttcgtccc agaaattgcc
      121 gaacacacga taggtcgaga cgcacagcgc acaggcgagg acgcatagtg ctatcagcgc
      181 gcctaccgag cggcggggcg accagcggat ctgcaaaacg cgccttccga gaatgaggaa
      241 atgtcgacaa gtttaacggc tgcgatgggt gtcgcaggag cgcttgtggg atattgcggg
      301 aataggtgag ggtcaagtag tgtgccgaag ttttttgccc ccgggattgc tcgatgacca
      361 tcaatgaccc caaggccctg ccgacgcccg cgtcctgggt ggtgcacaag tttggcggct
      421 cgagcgtcgc cgatgcgcag tgtttcgagc gcgtggccaa gatcgtggag tcgcagccgg
      481 cgcagcagct ggcgatcgtg ctgtcggcct gccggggcat caccgacgcg ctgctcgaac
      541 tggtatcgct ggcggaagcc cagcagccgg cctggcacga acgcctcgct gcggtgcgcg
      601 agcgccacgc cggcatcgcc accaccctgc tcaatgccga ggccgccgcc ggctacatga
      661 gccagttcga ccgcgatatg gaggacgtcg aaggggtgct gacgaccacg agcatcatgc
      721 gctcggccgc acagacggta cgcgatctgt gctcgggata cggagagatc tggtccaccc
      781 ggctgttcgt tcgctatctg cagcaacgcg gcgcgcgcgg cggtgcgcag tggatcgatg
      841 ccaggcgctg cgtgaccgtg gaatggggtc cactcggacc cgcggtgcaa tggcagagct
      901 cgcagctcaa gctgcagcag ctgctcgctc cccaggggtc gcagccagcg gcctcaaccc
      961 tggtgatcac cggtttcatt gccagcgatc cgcgcggcgt gcagaccacg ctcgggcgca
     1021 atggcagcga tttttcagcg tcgatcttcg gcgcgctgct tgaggccgcc gagattcaga
     1081 tctggaccga cgtcgatggc gtgctgtcgg ccgacccgcg ccgggtaccc gatgcccagg
     1141 tcatcgattc gctttcctac aacgaggcga tggagctggc gtacttcggc gccaaggtga
     1201 tccaccctca gaccatggcg ccggcggtgg gccgcgacat tccgatctgg atccgcaaca
     1261 cctttgcgcc cgagaaggca ggcacgctca tctgcgcccg accgacatcg acgctgccgg
     1321 tcaagggcat caccagcatc gaaggtgtcg cgctcatcaa cctcgagggt gcgggcatga
     1381 tcggggtgcc cggcaccgct catcgcctgt tcggcgcgct gcgcgaggag tcgatctcgg
     1441 tgatcctgat ctcccagggc agttccgagc attcgatctg ctgtgcgatt ccgcaaaccc
     1501 aggccgagcg tgcgaagaac gtgctcgagg cggcgttcgc gcgtgaactg accgaagggc
     1561 agattcagag cgtggacatc acgcaggatc aggcgatcct cgcggtggtc ggcgacggca
     1621 tgaccggcat gccgggtgtg tccggcaagc tcttcaacgc tctgggcaac acgcatatca
     1681 atgtgcgggc catcgcgcag ggtgcctcgg agcgcaatat ctcggtggtg gtcgacggca
     1741 aggacgccac ccgcgcactg cgggccacgc acgccagttt ctatctgtcg ccgcacacgc
     1801 tgtcgatcgg tgtgatcggt ccgggtaccg tcggccaggt gctgctcgat cagctcgcca
     1861 gtcagcgcga acggctggcg cgtgaattca agatcgatct gcgcgtgcgc ggcgtgctgc
     1921 gttcgcgcca gatgcgcctg accgacgtcg ggctgcagtt gccgcactgg cgcgcggagc
     1981 tgctgtcggg ttcgcagccg gcggacctgg cgcgcttcgt cgaacacgtg cgcgtggact
     2041 acctgccgca tacggtgctc atcgattgca gtgccgatgc cgaggtcgcc agtcactatc
     2101 acgaatggct ggcggccggc attcacatcg tgacgccgaa caagaaagcc aacagcggct
     2161 cgctggccta ttatgagagc ctgaaggcgg ctcgccgcgc cagcggcgcg cactatctgt
     2221 acgagaccac ggttggcgca ggacttccgg tcatccagac gctgcgcgat ctgcgcgaga
     2281 ccggtgatga gatcaccagc atcgaaggga tcttttccgg aacgctggcg tatctgttca
     2341 acgtctacga tggtactcag ccgttctcgt cgatcgtgcg cgaggccaag cagcgcggct
     2401 acaccgaacc ggatccacgc gatgatctgt ccggcaccga tgttgcgcgc aagctcatca
     2461 tcctcgggcg tgagatgggc ctgaaactcg aactgcgcga tgtccaggtc gcaagtctgg
     2521 tgccctccga cctggtcagc ggcagcagtg aggactttct gagcggactg ccgcgccacg
     2581 acgcagtgat gaagcgtcag tacgagggtg cgcgttcgcg cgaccgcgtg ctgcgctaca
     2641 tcggggcgct gtcggccaac ggcacagcga cggtcggcct ggtcgagctc gatgaaagac
     2701 atccgttcgc caatatcgcg ctgaccgaca atgtcgtgcg ttaccagacc gcgcgctatt
     2761 gcaacaatcc gctgatcgtg caggggccgg gtgccggccc ggaagtcacg gccgggggtg
     2821 tgttcgccga tctgctgcgt ctggccacct acctgggagc gcgcctgtga cgcgggcaca
     2881 ggccaccgcg ttcgcgccgg cctcggtcgg caacgtttcg atcggcttcg acatccttgg
     2941 cttttcggtc gatgcgctcg gggattgtgc gaccgttcga cgcaccgatt cccccggggt
     3001 cacgatcagg gcgatccgcg gtgtggtgca ggacctgccg atggaagccg cgaagaacac
     3061 tgccgggcgc gccatcatcg ccatgcactc ggcgctc
//
LOCUS       DATMRM010000273         3855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4184717, whole genome shotgun sequence.
ACCESSION   DATMRM010000273 DATMRM010000000
VERSION     DATMRM010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4184717"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VNZ06_07945"
     CDS             <1..254
                     /locus_tag="VNZ06_07945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWW20720.1"
                     /translation="LEFSQPVVPVLARLYDAYSFNVLPWLGKAVTGDADSYRYLAESI
                     RRHPDQETLLRLMRTAGFEDCRYHNLMAGIVAVHRGYRY"
     gene            257..868
                     /locus_tag="VNZ06_07950"
     CDS             257..868
                     /locus_tag="VNZ06_07950"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HWW20721.1"
                     /translation="MSSVADIAIERLLGHAVARARSDSPRAVALMAELAGRKLRVQVL
                     GTPLALRLECDGQALHLCPDQHTDAAIVGAPLSLLALAGEDPQAVITRGDVTIGGDAQ
                     IAQGFRELGMLLRPDLEAGLSQLFGRSGAHVAMQGLRVAGDWARATAWTATQNVAEYL
                     AHERGDLVSRAEAEHFLRGVEQLREQVDRLDARLSHLEGHTKN"
     gene            868..2520
                     /gene="ubiB"
                     /locus_tag="VNZ06_07955"
     CDS             868..2520
                     /gene="ubiB"
                     /locus_tag="VNZ06_07955"
                     /inference="COORDINATES: protein motif:HMM:NF003404.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HWW20722.1"
                     /translation="MRLRALARLIEIQRVLVRHRLDDFVRATHLYRPLRFVFFISPWT
                     WFQRRSGLPAAARLRLALEELGPIFVKFGQALSTRRDLLPDDIADELAKLQDRVPPFP
                     GAIARAMIEQSYGVPLHTRLQSFDEAPLAAASIAQVHAAQLPDGREVIVKVLRPGMQS
                     TIALDIEVLYTIAGLAQRWWTESRRLKPLEIVREYEKTILDELDLLREAANASQLKRN
                     FAGSNLLYVPEIHWDHCRKDVLVMERIRGIPIGELERLREAGTDFRRLAENGVEIFFT
                     QVFRHNFFHADMHPGNIFVLLDDPKQPRYAAVDFGIVGTLDLRDQRYLAENFLAVFDR
                     DYRRVATLHVQSGWVPPDTRIDEMESAIRTICEPIFDRPLKEIYFGAVLLRLFEALRR
                     FNGQIQPQLLLLQKTLLNVEGLGRQLYPELDIWQTASPVLRRWMRERLSARNVLRELR
                     RGLPDVLEVLRVLPPLSKRTLEQFQDGQLIFPVDSSAVDRLRAEWLDQRRRSDAISAG
                     AVLLLGALLWFALRVEMRWIGFILAALGFGSWLVAWRRRRRD"
     gene            2566..>3855
                     /locus_tag="VNZ06_07960"
     CDS             2566..>3855
                     /locus_tag="VNZ06_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWW20723.1"
                     /translation="MYPNTPARFVLALLIAALGVTATTRAQQAKPQYPDYPSETPSKF
                     EAPTYGLDHEMRNVMIAMRDGVKLHTIILVPKGAKHVGILLTRTPYEAKHLAANQHSV
                     HLGPRLWGYDNATETIVEGGYIRVIQDIRGKYLSEGDYVMNRPPARGPINPTPVDETT
                     DTYDTIDWLVKNVPESNGRIGILGISYDGFLAAMPLVNPHPALKVAVPMNPMVDGWRG
                     DDWFHNGAFRQQNIPYIYEQTATRANDELWQSSNFDDYDMYMRAVSAGELGKERGMNQ
                     IGFWNKLTDHPAYDRFWSEQAVDKILAKQPLTVPTMLVAGLWDQEDIYGALAAYKALL
                     ANPANRDNLFYVLGPWYHGQEIEEASALGAIKWGSDTGLYFRREILAPFLAHYLNDDA
                     PAMHVAPVTAFETGSNHWQQLQTWPSGCPRGCEWKATP"
BASE COUNT          707 a         1222 c         1223 g          703 t
ORIGIN      
        1 tactcgagtt ctcgcagccc gtggtcccgg tgctggcccg tctgtacgat gcgtattcct
       61 tcaacgtgct cccgtggctc ggaaaggccg tcaccggcga tgccgacagc taccgctatc
      121 tcgccgaatc catccgccgt catcccgacc aggaaacgct actgcggctg atgcgaaccg
      181 caggcttcga agattgccgc taccacaatc tgatggcggg catcgttgcc gtccatcgcg
      241 gctatcgcta ttgagtatga gcagcgtcgc tgacatcgcc atcgagcgtc tgctcgggca
      301 cgcggttgca cgggcgcggt cggattcacc gcgcgcggtc gcgctgatgg ctgagcttgc
      361 cgggcgcaag ctccgggtac aggtgctggg cacgccgctc gcgctacggt tggaatgcga
      421 tggacaggca ttgcatctgt gtccggacca gcacactgac gccgcgatcg tcggtgctcc
      481 gctgtcattg ctcgcactcg cgggcgagga tccgcaggcc gtcatcactc ggggcgacgt
      541 cacgatcggc ggcgacgcgc agatcgcgca ggggttccgc gagctgggca tgctgctgcg
      601 accggatctg gaagccggcc tgtcgcagct gttcggtcgc agcggggcgc atgtggcgat
      661 gcaggggttg cgtgttgcgg gcgactgggc gcgtgccacc gcgtggaccg ccactcagaa
      721 cgtcgccgaa tatctcgcgc atgagcgcgg cgatctggta tcacgcgccg aagcggaaca
      781 cttcctgcgt ggcgtggagc agctgcgcga acaggtggat cggctcgatg cccgcctgag
      841 tcatctggaa ggtcatacga agaactgatg cgtctgcgcg ctctcgcccg cctgattgag
      901 atccagcgtg tgctcgtgcg ccatcggctg gatgacttcg ttcgcgcgac gcatctttat
      961 cggccgctgc ggttcgtctt cttcatctcc ccgtggacct ggtttcagcg tcgttctgga
     1021 ctgccagcgg cggcacgact gcgcctggcg ctggaagagc tcggtccgat attcgtcaag
     1081 ttcggtcagg cattgtcaac gcgtcgcgat ctgctgcccg acgacatcgc cgatgagctg
     1141 gcgaaactgc aggatcgggt accgcccttt ccgggcgcca tcgcgcgggc aatgatcgaa
     1201 cagtcctacg gagtaccgtt gcacacccgc ttgcagtcgt tcgacgaggc gccgctggcc
     1261 gccgcctcga tcgcccaggt gcacgccgcg cagctgccgg acggacgcga agtgatcgtc
     1321 aaagtgctgc gtcccggaat gcagtcgacg atcgcgctgg acattgaagt gctgtacacg
     1381 atcgccggcc tggcacagcg ctggtggacc gaaagccgac gtctcaaacc actggagatc
     1441 gtgcgcgagt acgaaaagac gatcctcgat gaactcgatc tgctgcgcga ggccgccaat
     1501 gcctcgcagc tcaagcgcaa tttcgccggc tcaaacctgt tgtacgtacc cgagatccac
     1561 tgggatcatt gccgcaagga tgtgctggtc atggagcgca tccgcggcat tccgatcggg
     1621 gaactcgagc gcctgcggga ggccggcacc gattttcgcc ggcttgcaga aaacggcgtg
     1681 gagatctttt tcacgcaggt gttccgtcac aacttctttc atgccgacat gcacccgggc
     1741 aacatcttcg tgctgctcga tgatccgaag cagccgcgct atgcggccgt cgacttcggc
     1801 atcgtcggca cgctcgacct gcgcgatcag cgttacctgg cggagaattt cctggcggtg
     1861 ttcgatcgcg actaccggcg cgtcgcgaca ctgcatgtgc agtcaggctg ggtgccgccc
     1921 gacacgcgca tcgatgaaat ggaatcggcc atccgcacga tctgtgagcc gatcttcgac
     1981 cgcccgctca aggagatcta cttcggagcg gtgctgctgc gcctgttcga ggcactacgc
     2041 cgtttcaacg gccagattca gccacagttg ctgctgctac agaagacgct gctcaatgtc
     2101 gaaggactcg gccgccagct ctatcccgag ctcgacatct ggcagaccgc cagcccggtg
     2161 ttgcgacgct ggatgcgcga gcgcctgagc gcgcgcaatg tgctgcgcga gctgcgtcgc
     2221 ggtctgcccg atgtgctgga ggtcctgaga gtcctgccgc cgctcagcaa gcgcacgctg
     2281 gagcaattcc aggacggcca gttgatcttt ccggtggaca gcagcgccgt ggacagactg
     2341 cgggcggagt ggctcgacca gcgacgtcgc agcgatgcca tcagcgccgg cgccgtcctg
     2401 cttctggggg cgctgctgtg gttcgcactg cgcgtggaga tgcgctggat cggattcatt
     2461 ctcgccgctc tggggtttgg tagttggctg gttgcctggc ggcgccggcg tcgcgactga
     2521 aacccgcgtg ctagactggc acgcaaacga tcagggagaa aagtcgtgta tccgaacacg
     2581 cccgccagat ttgtgctcgc cctcctgatc gccgccctgg gtgtcaccgc gacgactcga
     2641 gcgcaacagg caaagcctca atacccggac tacccgagcg agacgccttc gaaattcgaa
     2701 gcgcccacct acggcctgga tcacgagatg cgcaacgtga tgattgcgat gcgcgacggc
     2761 gtgaaactgc acacgatcat tctggtaccc aagggcgcca aacatgtcgg aatactgctg
     2821 acgcgcacgc cctacgaagc caagcacctc gcggccaacc agcacagcgt gcacctgggg
     2881 ccccgcctat ggggctatga caacgccacc gagacgattg tcgagggcgg ctacatccgt
     2941 gtgattcagg acatccgcgg caaatacctc agtgagggcg actatgtcat gaatcgcccg
     3001 ccagcccgcg ggccaatcaa tccgacgccg gtggatgaaa ccaccgacac ctacgacacg
     3061 atcgactggc tggtgaagaa cgtgccggag tccaatggtc gcatcggtat cctgggaatc
     3121 tcgtacgacg gcttcctggc agcgatgccg ctcgtcaacc cccatcccgc actgaaggtc
     3181 gcggtaccga tgaatccgat ggtggacggc tggcgtggtg atgactggtt ccacaacggc
     3241 gcgttccgcc agcagaacat tccctacatc tatgagcaga ccgcgacgcg cgccaacgat
     3301 gagctgtggc agtcgagcaa tttcgacgac tacgacatgt atatgcgcgc ggtgtcggcg
     3361 ggtgagctgg gcaaagagcg cggcatgaac cagatcggat tctggaacaa gctgacggac
     3421 catccagcgt acgaccgttt ctggagcgag caggcggtcg acaagatcct ggcgaagcag
     3481 ccgctcaccg tcccgacgat gctggtggcc ggcctgtggg atcaggaaga catctacggc
     3541 gcgttggcgg cctataaggc gctgctggcc aatccggcca accgcgacaa tctgttctac
     3601 gtgctgggcc cctggtatca cggacaggag atcgaagagg ccagcgcgct cggcgcgatc
     3661 aagtggggca gcgacaccgg cctgtatttt cgccgcgaga ttctggcacc cttcctggcg
     3721 cactatctga acgatgacgc accggccatg cacgtcgcgc cggtgaccgc gttcgaaacc
     3781 ggcagcaatc attggcagca actgcagacc tggccctccg gctgccccag aggctgcgaa
     3841 tggaaggcca cacca
//
LOCUS       DATMRM010000274         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4510006, whole genome shotgun sequence.
ACCESSION   DATMRM010000274 DATMRM010000000
VERSION     DATMRM010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4510006"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1626)
                     /locus_tag="VNZ06_07965"
     CDS             complement(106..1626)
                     /locus_tag="VNZ06_07965"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HWW20724.1"
                     /translation="MQDRRLRSILIVGGGTAGWMAAAALAKVLGRECSIRLIESEEIG
                     TVGVGEATVPHLKLFNSVLEIDEVEFIREVRGTFKLGIQFVDWGRAGDRYIHGFGTIG
                     HDYGLLPFHQYWLKQFLAGQATDIGAYSLNTAAAPLGKFMSSAQDVPASSPLANITYA
                     YHFDAGLYALYLRRYAEARGVRRTEGKVVDVRLRSTDGFIEAVVLEGGEQVSADLFID
                     CSGFRGLLIEQALHTGYEDWTHWLPCDRAMAVPCENVGPPTPYTRSTARAAGWQWRIP
                     LQHRTGNGYVYSSAYIKDDEAADTLMSNLDGRALAEPRKLRFTTGMRRKFWNRNCVAV
                     GLASGFMEPLESTSIYMIQSGIARLINLMPDRSFSPVLIERYNKQAAFECERIRDFLI
                     LHYFATERRDSPFWQYCSSMEIPAQLADNIRLFRDSGRFFRDAEEMFAITSWVQVMLG
                     QHILPRNYHPAVDLIPERELNELVVGVRNVIASCVEAMPMHAQFLARYGGATRQQP"
     gene            complement(1689..>2514)
                     /locus_tag="VNZ06_07970"
     CDS             complement(1689..>2514)
                     /locus_tag="VNZ06_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20725.1"
                     /translation="HLKPPYSDQFSIGMRNRVGDWNTSATVSRVLSYDGFALTLGNRF
                     PNGSFWMNGGQPWGNGIPGFGSLILGGSGFERKATEVLLSAEKPYTEESGWGTTFAYT
                     FTDAKQNHIHIDDCCAFDEETVKQYPWITSNAAARHRLVATGSLRGPWELIFGGKLTV
                     ASPLPFTDLACGILKPGTFYPTGSSCTPISVTPLDGVGFSSLDLQVTKNFMVYREQTG
                     YVRVDLINALNSANYKDIITNYGSNGVANPHPVKYNTTGNVLGAPQTLRLTVGYKF"
BASE COUNT          460 a          787 c          803 g          464 t
ORIGIN      
        1 atcgctgttg cgccggctgt gcgtctgaac ccggaggcgt cgcgcatccg ccgatcaacg
       61 cgcccaccag aatcggggtc agacggcatt tcatctccgc gatcctcatg gctgctgtct
      121 ggtcgcccca ccgtacctgg cgaggaactg ggcgtgcatg ggcatggcct ccacgcagct
      181 cgcgatgaca ttgcgaacgc cgacgacgag ttcgttcagc tcccgctcgg ggatcagatc
      241 gacggccgga tgataattgc gtggcaggat gtgctgcccg agcatgacct gcacccagct
      301 cgtgatggcg aacatttcct cggcatcgcg gaagaagcgc ccactgtcgc ggaacaggcg
      361 gatgttgtcg gccagctgcg ccggaatctc catgctgctg cagtactgcc agaacggcga
      421 gtcgcgccgc tcggtcgcga agtagtgcag gatcagaaag tcgcggatcc gctcgcactc
      481 gaacgccgcc tgcttgttgt aacgctcgat cagcaccgga ctgaagctgc ggtccggcat
      541 caggttgatg agcctcgcga taccggactg gatcatgtag atgctggtcg actcgagcgg
      601 ctccatgaaa ccgctcgcga gcccgaccgc cacgcagttg cggttccaga acttcctgcg
      661 catgccggtc gtaaacctga gtttccgcgg ctcggccagc gcgcgcccgt cgagattgct
      721 catcagggtg tccgctgcct catcgtcctt gatgtaggcg ctcgagtata cgtagccgtt
      781 gccggtgcgg tgctgcagcg ggatgcgcca ttgccagccg gccgcgcgcg cggtggagcg
      841 cgtgtacggc gtggggggac ccacgttctc gcatggcacc gccatggcgc gatcgcaggg
      901 cagccagtga gtccagtcct cgtagccggt gtgcagggcc tgctcgatca gcaacccccg
      961 aaagcccgag cagtcgatga agagatcagc gctcacctgc tcaccgccct ccagcaccac
     1021 cgcttcgatg aagccgtcgg tgctgcgcag ccgcacatcc acgaccttcc cttcggtgcg
     1081 ccgcacgccg cgcgcttcgg cgtagcggcg cagatacagg gcatagagac ccgcgtcgaa
     1141 gtgataggca taggtaatgt tcgcaagcgg cgagctcgcc ggcacgtcct gcgcggatga
     1201 catgaatttc ccgagcgggg cggcggcggt gttgagcgag taggcgccga tgtcggtggc
     1261 ctggccagcg aggaactgct tcagccagta ctggtgaaac ggcagcagcc cgtaatcatg
     1321 gccgatggtg ccgaagccgt ggatgtagcg atcgccggca cggccccagt cgacgaactg
     1381 gatgccgagc ttgaaagtgc cgcgcacctc gcggatgaac tcgacctcat cgatctccag
     1441 aacgctgttg aagagcttca ggtgcggtac ggtggcttcg cccacgccga cggtgccgat
     1501 ctcctcggat tcgatgaggc gaatggagca ctcgcgcccc agcaccttgg cgagcgcggc
     1561 ggccgccatc catcctgcgg tgccgccgcc gacgatgagg atgctgcgga ggcgacgatc
     1621 ctgcatgagg tctcagaagc gcaccctcgg cggagttcgc atcgcgaact ccgccgggca
     1681 cgcgcacttt agaacttgta gcccaccgtc aggcgcagcg tttgcggtgc gcctaggacg
     1741 ttgccagtgg tgttgtattt taccggatgg gggttcgcca ccccgttcga gccataatta
     1801 gtgatgatgt ccttatagtt agccgagttc agcgcgttga tcagatccac gcgcacatac
     1861 ccagtctgtt cccggtagac catgaagttc ttggtcacct gcagatcgag ggaactgaag
     1921 cccacgccgt ccaacggcgt cacggaaata ggcgtgcaac tgctgcccgt ggggtagaac
     1981 gtgccgggct ttagaatccc gcaggcaagg tcggtaaaag gaagcggcga tgccactgtc
     2041 agcttgccgc cgaagatcag ttcccaggga ccgcgtaagg atcccgtagc caccaagcga
     2101 tgcctggccg ccgcgttcga agtgatccag gggtattgct tgacggtctc ctcgtcgaac
     2161 gcgcagcaat cgtcgatgtg gatgtggttc tgcttcgcgt cggtgaaggt gtaagcgaac
     2221 gtggtccccc agccggattc ctcggtgtag ggtttttcgg ccgacagcaa tacctcggtg
     2281 gctttgcgct cgaagccgct gccgcccagg atcagcgaac cgaagccggg gatgccattg
     2341 ccccagggtt gaccgccgtt catccagaac gagccattgg gaaaccggtt acccagagtc
     2401 aaggcaaagc cgtcatagct caatacccgc gacaccgtcg cgctggtgtt ccagtccccc
     2461 accctgttgc gcatgccgat gctgaactga tccgagtacg gcggcttcag gtga
//
LOCUS       DATMRM010000275         7124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3874770, whole genome shotgun sequence.
ACCESSION   DATMRM010000275 DATMRM010000000
VERSION     DATMRM010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3874770"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /gene="murD"
                     /locus_tag="VNZ06_07975"
     CDS             <1..860
                     /gene="murD"
                     /locus_tag="VNZ06_07975"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HWW20726.1"
                     /translation="ELSSFQLETTESLALAAATVLNVTPDHLDRYPDVRSYAAAKARI
                     FRHCEVAIINLDDPLVAAMVDRGRRRLSFSLRADVGADFALRRADDGQWWLARAGEKL
                     LALSQMKINGLHNAANALAALALGEAVGLTRAACVQALSEFSGLPHRAQWVGEIGGVR
                     YINDSKGTNVGATIAAVDGMTGPLIVIAGGDGKGQDFAPLRAAFAGKVRAVVLIGRAA
                     GALQSVLEAVTETHLAGTLDEAVDVAAYLAHPGDTVLLSPACASLDMFRDYAHRGSVF
                     TAAVQRRSA"
     gene            857..2032
                     /gene="ftsW"
                     /locus_tag="VNZ06_07980"
     CDS             857..2032
                     /gene="ftsW"
                     /locus_tag="VNZ06_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HWW20727.1"
                     /translation="MSSAVETMTRSERSQLGLAIDGPTLMLTLALLLLGLTMVTSASI
                     TIAGRDGMPFAFLERQLLLVLIGGVAAMLTFAIPTQTLERFALPLLLVALALLFVVLI
                     PGLGHTVNGSRRWLHIGALNFQVSEAARVLALIYIASYAVRYEEDLRSGLSGVIRPLG
                     LLAAIAFLLLLEPDFGAGCVLFLTGFGLLFLAGAQWRWILATVLFAGSAMWLLVRFSP
                     YRLQRLLTFMDPWADPYHSGFQLTQSLIAIGRGGLFGVGLGASVQKLFYLPEAHTDFL
                     FAVLAEELGLAGVALTLGLFLGLAWRALVIARRAHDAGLKFQSYLAAGFGLWLGIQAF
                     INVGVNMGVLPTKGLTLPFMSYGRSSLIVTLAWLGIVLRVHHETVHGKRRSGRAERA"
     gene            2029..3132
                     /gene="murG"
                     /locus_tag="VNZ06_07985"
     CDS             2029..3132
                     /gene="murG"
                     /locus_tag="VNZ06_07985"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HWW20728.1"
                     /translation="MSTALKPSRLALIMAGGTGGHVFPALALARELRARSWQVVWLGT
                     RRGLEARLVPPEQIPLEWLTVSGLRGKGVLVWLTAPLKLMRALAQALAVIRRRRPALV
                     VGLGGFVAGPGGLAAWLLRKPLLIHEQNAVAGFTNRCLSHLARRVLEAFPGSFGAGVK
                     AQAVGNPVRREITQLPPPSQRFAGRQGAIRVLVVGGSQGAAKLNSIVPFALARTRAAV
                     ALEVLHQSGERWIEAARDSYAKAGVSARLQPFIEDMAEAYGWADLVVCRSGALTVSEL
                     AAAGVGSVLVPFAAATDDHQTCNASYLVTAGAAVMIAERELDVERLACELERLCADRS
                     TLSVMADRARSLASPRAAEQLADACVELAGGVA"
     gene            3129..4571
                     /gene="murC"
                     /locus_tag="VNZ06_07990"
     CDS             3129..4571
                     /gene="murC"
                     /locus_tag="VNZ06_07990"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HWW20729.1"
                     /translation="MSAHMNPQGMARARMRRIDTIHFIGIGGSGMGGIAEVLLNLGYR
                     VQGSDLKVNAVIEHLRALGANVAIGHRAENVERADVVVVSSAVSRDNPEIAVALSRRI
                     PVVPRAEMLGELMRFRESIAVAGTHGKTTTTSLIASVLAEGGEDPTFVIGGRLNSSGS
                     NARLGDGRYLVAEADESDASFMHLQPVIAVVTNIDNDHLGTHGGDFNKLRHSFVEFLH
                     NLPFYGLAVLCGDDPNVQSILGQVGRSIVTYGLNEGADVRATRLERDRNGTRFDVEMS
                     GRVRPLSVLLNLPGEHSVRNALAAIVVANELGVADGAICRALASFQGIDRRMQYLGDA
                     KVAGGSVSIVDDYGHHPTEIAATIEALRQAWPGRRLVLVFQPHRYTRTRDLLDDFAKV
                     LSDMEVLIVTEVYPAGEAPIKGADGRAICRAVRGRGKVEPIFVEKVEQIDEALRDVVQ
                     AGDVVVTMGAGHIGAIAHELPAKLSAGGSQ"
     gene            4568..5554
                     /gene="murB"
                     /locus_tag="VNZ06_07995"
     CDS             4568..5554
                     /gene="murB"
                     /locus_tag="VNZ06_07995"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HWW20730.1"
                     /translation="MKFRITGDFNGRVRYDEPMSTHTSWHAGGPADVFFTPRDADDVS
                     SFLRMLPAEVPVYWVGLGSNLLVRDGGVRGVIVSTPGAFTRIERRSQTRIYVEASVPC
                     ARVARSCQNWGLSQSEFLGGIPGTLGGALAMNAGSFGDETWRHVNAIESIDRRGTRRR
                     RRRDEFKVGYRSVEWPVSELQEWFLSADLQFEPRAPAQTPTVQSLLQRRRETQPTGDW
                     SCGSVFKNPPGDHAARLIEAAGLKGYRIGDAVVSEKHANFIVNEGKAHASELEKLILH
                     VRDTVQELYSIALEPEVRIIGEALATPMSVSDAASSTHSDVNSPSISNGGRC"
     gene            5554..6528
                     /locus_tag="VNZ06_08000"
     CDS             5554..6528
                     /locus_tag="VNZ06_08000"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HWW20731.1"
                     /translation="MRLRYDPRAAYRVTQPADFGQVAVLYGGSSAEREVSLISGKAVS
                     AGLVRRGISVTPFDPAERALSDLQSLKIDRVWIALHGPGGEDGTLQGALEFLGTPYTG
                     SGVMGSAIGMDKLRSKRLAQSLGIPTAEFVVLHEARDLDTALERLGLPMIVKPGSQGS
                     SVGMARVEKAQDLPEAWNNALRVDPVVFAESWIGGGEYTVSILQGQALPSIRIETPRA
                     FYDYEAKYLRDDTKYHCPSGLSAQAEQHLAKLALASFEACGAEGWGRADFMMDKTGRA
                     LLLEINTVPGMTDHSLVPMAARAAGIDFDELVWRVLETSLVRIPIDTP"
     gene            6525..>7124
                     /locus_tag="VNZ06_08005"
     CDS             6525..>7124
                     /locus_tag="VNZ06_08005"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HWW20732.1"
                     /translation="MSTLNRKRNSRRASTRERRSGLALAALNWGRIGLSLSALAVTGL
                     AVLALGWLLDQPIQRVIVSGRLQRVSAMDVEKVVRARLGGAGLVTVNLAAISRGLIQL
                     PWVEQAAVMRSWPRGLKIEIVEQTAVARWNNAGLLNANGDLFVSESRFIPPELPTLSG
                     PAGSEVEVTQRYVSTQERLTEIGMRLAALELDARGAWNLT"
BASE COUNT         1151 a         2156 c         2495 g         1322 t
ORIGIN      
        1 tcgagctttc tagttttcaa ctcgagacca ccgagtcact cgcgctcgcc gccgcaacgg
       61 tgctcaacgt cacgccggat catctggatc gctatcccga cgtgcgcagc tacgccgccg
      121 ccaaggcgcg cattttccgg cactgcgagg tcgcgatcat caatctggat gatccgttgg
      181 tcgcggcgat ggtcgatcgc ggtcggcgcc gcctgagctt ctcgttacgc gccgatgtgg
      241 gcgcggactt cgcgctgcgc cgcgcggacg acggccagtg gtggctggcg cgcgccggtg
      301 agaagctgct ggcgctatcg cagatgaaga tcaacggact gcacaacgcc gccaacgccc
      361 tcgcggccct ggcgctcggt gaggccgtgg ggctcacgcg cgcagcctgc gtgcaggcac
      421 tgagcgaatt cagcggattg ccgcatcgcg cgcagtgggt cggcgagatt ggcggtgtgc
      481 gctatatcaa tgactccaag gggaccaatg tcggcgcgac gatcgcagcc gtggacggca
      541 tgacaggtcc gctgatcgtg atcgccgggg gcgacggcaa gggtcaggac tttgcaccac
      601 ttcgtgcggc cttcgccggc aaggtgcgcg cggtggtgct catcggccgc gccgcgggcg
      661 ccctccagag cgtactggaa gctgttaccg agacgcatct ggcaggcacg cttgatgaag
      721 ccgtggacgt ggccgcatac ctggcgcatc cgggtgacac agtactgctg tcgccggcct
      781 gcgcgagcct ggacatgttc cgtgactatg cgcatcgcgg cagcgtgttc accgctgctg
      841 tgcagaggcg ttcagcgtga gcagcgccgt tgaaaccatg acccgatccg agcgctcgca
      901 actcgggctc gcgatcgacg ggcccacgct gatgctgacg ctggcgctgc tgctgctggg
      961 gttgacgatg gtgacctcgg cctcgataac catcgccggg cgcgatggca tgccgttcgc
     1021 gttccttgag cgacagctcc tgctggtgct gatcggcggc gtcgctgcga tgttgacctt
     1081 cgcgataccg acgcagacgc tcgagcgctt cgcgttgccg ctgttgctgg tcgcgctggc
     1141 gctgctgttt gtggtgttga taccggggtt gggccacacc gtgaatggca gtcgtcgctg
     1201 gctgcacatt ggcgcactca acttccaggt ctcggaagcc gcacgtgtgc tggcactcat
     1261 ctatatcgcc agctatgcgg ttcgttacga ggaagatctt cgcagtgggt tgtccggcgt
     1321 gatccgtccg ctcggtctgc tcgcggcgat tgcgttcctg ctgctgctcg agccggattt
     1381 cggtgccggc tgcgtgctgt ttctgaccgg gttcggcctg ctgttcctgg ccggtgcgca
     1441 gtggcgctgg attctggcga ccgtcctgtt cgccggcagc gcgatgtggc tgctggtgcg
     1501 cttctctcct tatcgattgc agcgcctgct gaccttcatg gatccgtggg ccgatcctta
     1561 tcacagtggt ttccagctca cgcaatcgct gatcgcaatc ggtcgcggcg gcctgtttgg
     1621 cgtgggactg ggcgccagcg tacaaaagct attttatctg cctgaggcgc acaccgattt
     1681 cctgtttgcg gtgctggccg aggagctcgg tctggcgggc gtcgcactga cgctgggcct
     1741 gtttctgggg ctggcgtggc gagcgctggt catcgcgcgc cgcgcgcacg atgcgggcct
     1801 gaagtttcag tcgtatctgg ccgccgggtt cggactttgg ctcggcattc aggcgttcat
     1861 caatgtcggc gtcaacatgg gggtgttgcc gaccaaggga ctgacgctgc ccttcatgag
     1921 ctacggacgc agcagcctga ttgtgaccct ggcctggctc ggaattgtgt tgcgtgtgca
     1981 ccatgagacc gtgcacggca aacggcgctc tggccgagcg gagcgagcat gagcaccgcg
     2041 ctcaagccct cacggctggc cttgattatg gcaggcggca ccggtggaca cgtgtttccg
     2101 gcgctggcgc tggcgcgtga attgcgtgcg cgctcgtggc aggtcgtgtg gctgggtacg
     2161 cgccgcggtc tggaagcgcg tctggtgccc ccggaacaga ttccgctcga atggctcacg
     2221 gtcagcggac tgcgaggcaa gggtgtactg gtgtggctga ccgcgccgct gaagctgatg
     2281 cgcgcgctgg cacaggcgct cgcggtgatc cgtcgcagac gtccggcgct ggtagtcgga
     2341 ctcggcggat tcgttgcggg tccgggagga ctggccgcgt ggttgctgcg caagccgctg
     2401 ctgattcatg agcagaatgc ggtcgcaggc ttcaccaacc gctgcctgtc gcatctcgca
     2461 cggcgcgtgc tcgaagcctt tcctggcagt ttcggcgctg gcgtcaaggc gcaggccgtg
     2521 ggtaacccgg tacgtcggga gatcacgcaa ttgccgccgc cgtcgcagcg cttcgctggc
     2581 cggcagggcg cgatccgcgt gctcgtggtc ggtggcagtc agggcgcggc caaactcaat
     2641 tcgatcgtac cgtttgcgct ggcacgcacg cgcgcggccg tcgcgctgga agtgctgcat
     2701 cagtccggcg agcgctggat cgaggcggcg cgcgatagct atgccaaggc aggggtcagt
     2761 gcacgcctgc aacccttcat cgaagacatg gcagaggcct acggctgggc ggatctggtg
     2821 gtctgccgct ccggcgcgct gaccgtgtca gagctcgccg cagccggcgt cggatcggtg
     2881 ctggtgccgt ttgcagccgc caccgacgat catcaaacct gcaacgcctc gtatctggtg
     2941 actgccgggg cggcggtgat gattgccgag cgcgagttgg acgtcgagcg gctggcgtgt
     3001 gagctcgagc ggctatgcgc cgatcgcagc acgttatcgg tgatggccga tcgggcgcgc
     3061 tcgctggcga gcccgcgcgc cgccgagcaa ctggccgacg cctgcgtcga actggccgga
     3121 ggtgtggcat gagcgcgcac atgaacccgc agggcatggc acgagcgcgg atgcgccgca
     3181 tcgatacgat tcatttcatt ggcattggcg gcagcggcat ggggggcatc gccgaagtgc
     3241 tgctcaatct gggctaccgc gtgcagggct cggatctgaa ggtgaatgcg gtgattgaac
     3301 atctgcgcgc gctgggcgcg aacgtcgcaa ttggtcaccg cgccgagaat gtcgagcgcg
     3361 ccgatgtcgt agtggtctcg agcgccgtct cgcgcgacaa cccggagatt gcggtcgcgc
     3421 tgtcgcgccg aattccggta gtgccgcgcg cggagatgct gggcgagctg atgcgttttc
     3481 gcgagtcgat tgcggtcgcc ggcacgcacg gcaagaccac gacgaccagc ctcattgcca
     3541 gtgtcctggc cgaaggcggt gaggacccga ccttcgtgat tggcgggcga ctcaacagca
     3601 gcggcagcaa cgcgcggctg ggcgacggtc gctatctggt ggccgaggct gatgagagcg
     3661 atgcctcgtt catgcatctg cagccggtga tcgcggtggt caccaacatc gataacgatc
     3721 atctcggcac tcacggcggc gacttcaaca agctcaggca cagcttcgtc gagttcttgc
     3781 acaacctgcc gttttacggg ctggcggtac tgtgcggtga cgatccgaac gtacagagca
     3841 ttctcggcca ggtagggcgc tcgatcgtaa cctacggtct gaacgaaggc gcggacgtgc
     3901 gcgccactcg cctcgagcgc gatcgcaacg gcactcgatt tgacgtggag atgtccgggc
     3961 gtgtgcggcc gctgtcggtg ctgctcaacc ttcccggcga acacagcgtg cgcaacgccc
     4021 tggcggcgat cgtggtggcc aatgagctcg gggttgctga cggcgcgatc tgtcgcgcac
     4081 tcgccagctt tcagggaatt gatcggcgca tgcagtacct gggcgatgcc aaggtcgccg
     4141 gcggcagcgt cagcatcgtc gatgactacg ggcaccaccc gaccgaaatc gcggcgacga
     4201 tcgaggcgtt gcgtcaggca tggccggggc ggcggctggt gctggtgttc cagccgcacc
     4261 gatatacccg aacgcgggat ctgctcgacg acttcgccaa ggtgttgtcg gacatggaag
     4321 tgctgattgt gaccgaggtg tatccggccg gcgaagcgcc gatcaaaggc gccgacggcc
     4381 gtgcgatctg tcgcgcggtg cgcggtcgcg gcaaggtgga gccgatcttc gtggagaaag
     4441 tcgaacagat cgatgaggcg ctgcgcgacg tcgtacaggc aggagacgtg gttgtgacca
     4501 tgggtgcggg acacattggt gccatcgcgc acgagttacc cgcgaagctg tcggctggag
     4561 ggagtcaatg aagttcagga ttaccggtga cttcaacggt cgagtgcgct acgacgagcc
     4621 catgtccaca cacacctcgt ggcacgccgg cgggcccgcc gacgtgttct tcaccccgcg
     4681 tgacgccgac gatgtgtcaa gttttctgcg aatgctgccc gctgaggtcc cggtgtattg
     4741 ggtgggtctg ggcagcaacc tgctggtgcg agacggtggc gtacgcggcg tgatcgtgtc
     4801 cacgcccgga gcattcaccc gcattgagcg acgctcgcag acccgcatct acgtcgaggc
     4861 cagcgtgccg tgtgcgcgcg tggcgcgttc ctgtcagaac tggggactgt cgcaaagcga
     4921 gtttctggga ggcattccgg gcaccctcgg tggggccctg gccatgaatg ccggctcttt
     4981 cggcgatgag acctggcggc atgtcaatgc gatcgagagc atcgatcggc gtggcacgcg
     5041 ccggaggcga cgacgcgatg aattcaaagt cggctatcgc tcggtcgaat ggccggtgag
     5101 cgagctgcag gaatggttct tgtcggccga cctgcagttc gagccgcgcg ctccggccca
     5161 gaccccgacg gtgcagtcgc tattgcagcg gcggcgcgag acgcagccca cgggcgactg
     5221 gagctgcggt tcggtgttca agaatccgcc gggcgatcac gcagcgcgcc tgatcgaggc
     5281 cgcagggctc aagggctatc gcatcggtga tgcggtggtg tccgagaagc atgccaattt
     5341 catcgtgaat gaaggcaaag cgcacgccag cgagctggaa aagctgatcc tgcacgtgcg
     5401 cgacaccgtg caggagctct atagcatcgc gctggagccg gaagttcgca tcattggcga
     5461 ggcgcttgcc acaccgatga gcgtcagcga tgccgccagc agcacccata gcgatgtcaa
     5521 cagtccctcg atcagcaacg gaggccgctg ctgatgcgat tgcgctacga ccctcgcgcg
     5581 gcctatcgag taacccagcc tgccgatttc ggtcaggttg cggtgctcta tggcggctcg
     5641 tcggccgagc gcgaagtctc gctgatcagc ggcaaggcag tgagcgcagg ccttgtgcgg
     5701 cgagggatca gcgtcacccc gttcgatccg gcggagcgtg cgctgagtga tttgcagtcg
     5761 ctcaagatcg atcgggtgtg gattgcgctg catggtcctg gcggcgagga cggcacgctg
     5821 cagggggcgc tggaattctt aggcacgccc tacaccggca gcggagtcat gggttcagcg
     5881 atcggcatgg acaagctgcg cagcaagcgc ctggcacagt cgctcggcat tccaaccgcg
     5941 gagttcgtcg tgctgcacga agcgcgcgac ctggacacgg cgctcgagcg gctcgggctg
     6001 ccgatgatcg tgaaacccgg ctcgcagggc tcgagtgtcg gcatggcgcg cgtggaaaaa
     6061 gcccaggatt tgcctgaggc atggaacaac gcgctgcgcg tggatccagt ggtgttcgcg
     6121 gaatcctgga ttggcggcgg cgaatacacc gtgtcgatcc tgcagggaca ggcgctgccc
     6181 tcgattcgca tcgagacccc gcgcgccttc tacgactacg aagcgaaata tctgcgcgac
     6241 gacacgaagt accactgccc ttcgggtctg tccgcgcagg ctgagcagca tctggcgaag
     6301 ctggcactcg ccagtttcga agcgtgcggg gccgaaggtt ggggtcgtgc cgacttcatg
     6361 atggacaaga ctggccgtgc gctgctgctg gagatcaaca cggtacccgg catgaccgat
     6421 cacagcctgg tgcccatggc cgcccgcgcc gcaggcatcg actttgatga gttggtctgg
     6481 cgtgtactcg agaccagtct ggtgcgcatc ccgatcgata cgccatgagc acactcaacc
     6541 gcaaacgcaa cagccgccga gcatcgacgc gcgagcgccg ctcgggtttg gcgctagcgg
     6601 cgctcaactg gggccgcatc ggcttgagcc tgtcggcact ggcagtgacc ggattggcag
     6661 tgctggcgtt gggctggctg ctcgaccagc cgatccagcg cgtcatcgtc agcggccgac
     6721 tgcaacgcgt gtccgcgatg gatgtggaaa aggtagtgcg cgcgcgcctg ggtggcgccg
     6781 ggctggtcac ggtgaatctg gctgcgatca gccgtggact gatacagctg ccctgggtcg
     6841 agcaggcagc ggtgatgcgt agctggccgc ggggattgaa gatcgaaatc gtcgagcaaa
     6901 ccgccgtggc gcgctggaac aacgccgggc tgctcaatgc caacggcgac ctgttcgtca
     6961 gcgagtcgcg cttcataccg cccgagctgc cgacgctttc gggacccgcc ggctcggagg
     7021 tggaagtcac gcagcgctat gtgagcacgc aggaacggct gacggagatc ggtatgcggc
     7081 tggcagcgct cgaactcgat gcgcgcggcg cctggaatct gaca
//
LOCUS       DATMRM010000276         6053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1402032, whole genome shotgun sequence.
ACCESSION   DATMRM010000276 DATMRM010000000
VERSION     DATMRM010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402032"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /gene="uraH"
                     /locus_tag="VNZ06_08010"
     CDS             <1..339
                     /gene="uraH"
                     /locus_tag="VNZ06_08010"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HWW20733.1"
                     /translation="THVLDISGGLPAAGIRIELLELNALTPKALLSTATGTDGRCPAP
                     LLAGADFRVGRYALRFHVAEYFRSRSVALADPPFIDEAVVHFGIAHPEQHYHVPLLIT
                     PWSYSVYRGG"
     gene            340..1812
                     /gene="xdhA"
                     /locus_tag="VNZ06_08015"
     CDS             340..1812
                     /gene="xdhA"
                     /locus_tag="VNZ06_08015"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HWW20734.1"
                     /translation="MSAADTVRFLLDGQLVNALDVPPTMTVLEYLRDVAHRTGTKEGC
                     AEGDCGACTVALGELSEDGTRVEFRAINSCIRPLPSIDGRELVTVESLSAPGAPLHPV
                     QQAMVDEHASQCGFCTPGFVMSLFALYLRTAAPTREQVLEALAGNLCRCTGYRPIIDA
                     ACRMGDYPPPPNWSRESQHSAQRCQWLRELRRERALQFPGWYSPRSIDELAAALHAQP
                     QALVLAGATDVGLWITKQLRELPAIIYVGEVAELKQIRRGADALRIAAAVSLTDAWDA
                     ITCDWPQLRELAQRFGSPPVRNAGTLCGNLANGSPIGDSMPVLIALGATLELRSVRGT
                     RQLPLEQFYLGYQRRDLQPGEFVSAVTIPAPQQDVQLASYKISKRFEQDISAVCAAFA
                     VRVRSGHVTAARIAFGGMAAVPSRAPATEQRLLGAPWSEQSIEAAVSALAQDFRPLSD
                     MRASEHYRLQAAANLLRRFFLESGGSTIALRTADAAAAMS"
     gene            1817..4135
                     /gene="xdhB"
                     /locus_tag="VNZ06_08020"
     CDS             1817..4135
                     /gene="xdhB"
                     /locus_tag="VNZ06_08020"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HWW20735.1"
                     /translation="MVAQNIEIEPSSRASPRHDSAHLHVSGRALYADDIALPANALHA
                     AFGMSSIAHGRIRKMDFAPLRQMSAVAAIAVASDVPGDNNYGSVLHDDPIFADGQVQY
                     AGQPLFAVAATSYNAARKAARAAIIDYEPLPAILDVRAALAAQSYVLPTERLLRGQPH
                     EMLGTAPRRLSGTVRIGGQDHFYLEGQIAIATPQEDGAMLVHSSTQHPSEVQQIVARA
                     LGRRSHDVTVQCRRMGGGFGGKETQPALIAAAAAVMAAKTQRAVKLRLDRDTDMLMTG
                     KRHDFIADYEVGFDDQGRILALSVMLASRCGYSADLSGPVNDRAMMHLDNAYYLEHIE
                     IISHRCKTHTVSNTAFRGFGGPQGMLVIEQILDDIARTLHLDPLSVRRCNFYGIGQRN
                     VTPYGQTVDDNLVHTLVDRVAASAQYALRRGSLAAWNTANPIIKRGIALTPVKFGISF
                     TSTIFNQAGALVHVYSDGTVLLNHGGAEMGQGLFIKVAQVVASELGVPLSAVRSSATD
                     TSKIPNTSATAASSGSDLNGKAAQAAAQTIRQRLVQFACDTYGVQAEELHFHDGRVDV
                     GSRQLTFAELVTQAYRARVSLSSTGFYRTPKIQWDKSAMRGRPFFYFAYGAAVSEVAI
                     DTLSGETQLLRVDILHDVGRSLNSAIDLGQIEGGFLQGTGWLTSEELWWNASGELQTH
                     APSTYKIPTARDWPACATVRLLEDAPNREETIYRSKAVGEPPLMLAISVLHAIRDAVA
                     SCAGQPTRVHLDAPATPEAVLRAVAAVRGIAR"
     gene            4132..5289
                     /gene="xdhC"
                     /locus_tag="VNZ06_08025"
     CDS             4132..5289
                     /gene="xdhC"
                     /locus_tag="VNZ06_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HWW20736.1"
                     /translation="MSALTGGIPCASVPETAGEQWLQPLRDWPHQALRMLQSEPAVVR
                     IVMATVQGSAPREAGVCMLVGQALVHGTVGGGQLEWQALSTARLMLGAERPSARIHRF
                     VLGGDIGQCCGGVVELWIERYTADDAPLLERARASTERGAALMRSELSSDGIERQVVC
                     DLGSGEESDRMLRSVRERAPPRLRRTALGAELLERLDNPLLPVWLYGAGHVGQALARA
                     LMPLPLRLTWIDSREDLLPKQVPDTVQCVHTLQPLESVASAPAGARFLVMTHSHSLDY
                     SLCRAILTRADFAWIGLIGSNSKSARFRSRLARHGVDESLIARLVCPIGVRGIDSKWP
                     AAIAIAVAAQLLQGISADAIDERIEAPPQAAAGSCGHGSCEDCHPSDKPVR"
     gene            5296..>6053
                     /locus_tag="VNZ06_08030"
     CDS             5296..>6053
                     /locus_tag="VNZ06_08030"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HWW20737.1"
                     /translation="MLSYLLDWLGLLVRWLHIVAGIAWIGASFYFVWLDNHLQPLRED
                     GPVDPNVAGELWAVHGGGFYRSQKYRVAPAQLPSTLHWFYWEAYTTWWSGFVLLCLLY
                     FFRAEAYLIDPGVVALSKPVAIGIALAVLVGSWLLYDLLCRSPLSRHSVALSSALGIV
                     FSAMAWGLCHVFSGRGAYMIFGTSIGTLMVANVAMVIIPGQRELVRARQQAREVDPAP
                     GMRGKQRSLHNTYFTLPVLFVMISNHYPMTYSHP"
BASE COUNT         1000 a         1891 c         2052 g         1110 t
ORIGIN      
        1 actcacgtac tggatatcag tggcggcctg ccggcggcag gcatccggat cgaattgctc
       61 gaactgaatg ccctcacgcc caaagctctg ctcagcacgg ccaccggaac ggacggccgc
      121 tgcccggccc cactgctggc gggtgcggat tttcgggtag gtcgctacgc gctgcggttt
      181 catgtggccg agtacttccg tagccgcagc gtggcgttgg ccgatccgcc tttcatcgac
      241 gaagcggtcg tgcatttcgg gatcgcgcat cccgaacagc actatcacgt gccgttgctc
      301 atcacgccct ggagctattc ggtctatcgg ggaggataga tgagcgcggc tgacacagtg
      361 cggttcctgc tcgacggtca gctggtcaat gcgctcgatg tgccgccgac gatgactgta
      421 cttgagtatc tgcgcgacgt ggcgcatcgc accggcacca aggagggctg tgccgaaggt
      481 gactgcggtg cctgcaccgt ggcgctcggt gaactcagcg aagacggcac gcgcgttgag
      541 tttcgcgcca tcaactcctg catccggcca ttgcccagca tcgatggccg cgaactggtc
      601 acggtcgaga gtctcagtgc gccgggcgcc ccattgcatc cggtgcaaca ggcgatggtc
      661 gacgagcatg cgtcacagtg tggcttctgt acgccgggct tcgtgatgtc gctgttcgcg
      721 ctgtacctgc gtacggcggc tcccacgcgc gaacaggtgc tcgaggcact agcgggcaat
      781 ctgtgtcgct gcaccggcta tcgtccgatc atcgatgccg catgccgcat gggcgattat
      841 ccgccgccgc cgaattggtc acgggaatcg cagcacagtg cgcagcgctg tcagtggctg
      901 cgcgagcttc gccgggagcg tgccttgcag tttccgggct ggtactcgcc gcgcagcatc
      961 gatgaactgg ctgcggcgct gcacgctcag ccgcaggccc tggtgctcgc cggcgcgacc
     1021 gatgtcgggc tttggatcac caagcagctg cgcgagctgc cggcgatcat ctatgtcggt
     1081 gaggtcgccg agctgaagca gatacgccgc ggcgcagacg ctctgaggat cgctgccgcg
     1141 gtctcgctga cggatgcctg ggatgcgatc acgtgcgact ggccgcaact tcgtgaattg
     1201 gcgcagcgct tcggttcacc accggtgcgc aacgccggca cgctgtgcgg caatctggcc
     1261 aatgggtcac cgatcggtga ttcgatgccg gtactgatcg cgctcggggc gacgctggaa
     1321 ttgcgcagcg tccgcggtac gcgtcagctg ccgctcgaac agttctacct gggctaccag
     1381 cgccgcgacc tgcagccggg cgaattcgtg agcgcagtga caatccctgc gcctcagcaa
     1441 gatgtgcagc tcgcgagcta caagatctcc aagcgattcg agcaggacat ttccgccgtg
     1501 tgtgccgcgt tcgccgtgcg cgtgcgttcc ggtcacgtca ctgcggccag gatcgccttt
     1561 ggaggcatgg ccgccgtacc cagtcgggcc ccggcgaccg agcagcggct gctgggcgcg
     1621 ccctggagtg aacagagcat cgaggccgcg gtcagcgcgc tggcccagga cttccggccc
     1681 ttgagcgata tgcgcgccag tgagcactat cggttgcaag ccgcggccaa tctgctgcgc
     1741 cgcttctttc tcgagtcggg cggctcgacg atcgcgctgc ggacggccga tgcggccgcc
     1801 gcgatgagct gacgtcatgg ttgcgcagaa catcgagatc gaaccgagca gccgtgcgtc
     1861 gccgcgccac gacagcgcgc atctgcacgt cagtggccgc gcgctgtacg ccgatgacat
     1921 tgcgctgccg gcgaacgcat tgcatgccgc gttcggcatg agcagcatcg cgcacggccg
     1981 gattcggaaa atggactttg ctccgttgcg gcagatgtcc gccgtcgcgg cgatcgcggt
     2041 cgcctctgac gtacccgggg acaacaacta cggcagcgtg ctgcacgatg acccgatctt
     2101 cgcggacggg caggtgcaat atgccggtca gccgctgttc gctgtcgccg caacgtccta
     2161 taacgcggcg cgcaaagccg cgcgcgccgc gatcatcgac tacgagccgc tgccagccat
     2221 tctggatgtg cgcgccgcgt tggctgcgca aagctatgta ctgccgacgg agcggctgct
     2281 gcgcggccag ccgcacgaga tgcttggcac ggcgcctcgt cggctgtccg gcacggttcg
     2341 aatcggcggt caggatcact tttatctcga gggacagatt gcgattgcca caccgcagga
     2401 agacggtgcg atgctggtac acagttccac acaacatccg tccgaggttc agcagatcgt
     2461 ggcgcgcgcg ctcgggcggc gttcgcatga tgtgacggtc cagtgccgcc gcatgggcgg
     2521 cggattcggc ggcaaggaaa cgcagccggc tctgatcgct gcggctgcgg cggtgatggc
     2581 ggccaaaacg cagcgcgccg tcaaactgcg gctcgatcgc gacaccgata tgctgatgac
     2641 cggtaaacgc cacgacttca tcgctgacta tgaagtggga ttcgatgacc agggccgcat
     2701 cctcgcactg agcgtgatgc tcgcctcgcg gtgcggctat tcggccgatc tgtccgggcc
     2761 ggtcaacgat cgcgccatga tgcatctgga caacgcttac tatctcgagc acatcgagat
     2821 catctcgcat cgttgcaaga cgcacaccgt ttcgaatacg gcgtttcgag gcttcggcgg
     2881 tccgcagggc atgctggtga tcgagcagat cctggacgac atcgcgcgca cattgcacct
     2941 ggatccgttg agcgtgcggc gctgcaattt ctatgggatc ggtcagcgta acgtgacgcc
     3001 gtatggccag acggtggacg acaacctcgt tcacaccctg gtcgatcgtg tcgccgccag
     3061 tgcgcagtac gcgctccgcc gcggcagcct tgccgcttgg aacacggcca atccgatcat
     3121 caagcgcggc atcgcgctga cgccggtaaa attcggcatt tcgttcacgt ccacgatatt
     3181 caatcaggcc ggcgcactgg tgcatgtcta cagcgatggc actgtgctgt tgaatcacgg
     3241 cggtgcggag atgggccagg gactcttcat caaggtggcg caggtggtgg cctccgaact
     3301 gggcgtgccg ttgtcggcgg tgcggtcatc ggccacggac acctcgaaaa tccccaacac
     3361 atcggccacc gcggcctcat ccggctcgga tctcaacggc aaggccgcgc aagccgcggc
     3421 acagacgatc cgtcagcgcc tggtgcagtt cgcgtgcgat acctatgggg tgcaagccga
     3481 agagctgcac ttccatgacg gccgtgtgga tgtcgggagc cgacaactga cgttcgcgga
     3541 actggtgaca caggcttacc gcgcacgcgt ctcgctgtcg tcgaccggct tctaccgaac
     3601 accgaagatc caatgggaca agagcgcgat gcggggccgg ccgttcttct atttcgcgta
     3661 cggcgcggcg gtcagtgaag tggcgattga tacactcagc ggcgaaaccc aactgctgcg
     3721 ggtggacatc ctgcacgatg tcggccgctc gctgaacagc gcgatcgacc tggggcagat
     3781 cgaaggcggg tttctgcagg gcaccggctg gctcaccagt gaggagttgt ggtggaacgc
     3841 aagcggtgaa ctgcagacgc atgcgccgtc gacctacaag attccgaccg cgcgcgactg
     3901 gccggcttgt gcaacggtgc gattgctcga ggatgcaccc aatcgcgagg agacgatcta
     3961 ccgctccaaa gcggtaggcg agccgccgct gatgctggcg atctcggtgc tgcacgcgat
     4021 ccgggatgcg gtggcaagct gcgcggggca acctaccagg gtgcacctgg atgcgccagc
     4081 aaccccggag gcggtgctgc gagcggtggc ggcggtcaga gggatcgcgc gatgagcgcg
     4141 ctgactggag gaataccttg cgcgtcggtg ccggaaacgg caggcgagca gtggctgcag
     4201 ccgctgcgcg actggccgca tcaggcgctg cgcatgctgc agagcgagcc cgcggtggtg
     4261 cgcatcgtga tggcaacggt gcagggttcg gcaccgcgcg aagccggcgt atgcatgctg
     4321 gtggggcagg cgctggtgca cggcacggtg ggtggaggac agctggagtg gcaggcgctc
     4381 agtaccgccc gcttgatgct gggcgccgag cggccgtcgg cgcgcataca tcggttcgta
     4441 ctcggcggcg acatcgggca gtgctgcggt ggtgtcgtgg aactgtggat tgaacgttac
     4501 acggccgacg acgcgccgct tctggaacgc gcgcgcgcga gcaccgagcg cggagcggcg
     4561 ctgatgcgca gcgaactgtc atccgacggg atcgagcggc aggtggtgtg cgatctggga
     4621 tcgggcgagg agtccgaccg gatgctgcgc agcgtgcgtg agcgcgcacc gcctcgcctg
     4681 cggcgcaccg cgctgggtgc cgagttgctc gaacggctcg acaatccgct gttgccggtc
     4741 tggctgtacg gcgcagggca cgtcggacag gccttggccc gtgcgctcat gccgctgccg
     4801 ctgcgactga cctggatcga ttcacgcgag gacctgcttc caaagcaggt accggataca
     4861 gtgcagtgcg tacacacgct gcaaccgctg gagagcgtcg ctagcgcgcc tgcgggcgcg
     4921 cgctttctgg tgatgacgca cagccattcg ctcgactatt cgctgtgccg ggcgattctg
     4981 acgcgggcgg atttcgcctg gatcggactg atcggctcga acagcaaatc cgcgcgtttt
     5041 cgatcgcggc tggcacgcca tggtgtggac gagtcgctga tcgcgcgtct ggtatgcccg
     5101 atcggtgtcc gggggatcga cagcaagtgg ccggctgcga tcgcgatcgc cgtcgcggcg
     5161 cagcttctgc agggcatcag cgccgatgcg atcgatgaac ggatcgaggc gccgccgcag
     5221 gccgccgctg gctcttgcgg ccatggcagc tgcgaagact gtcatccgtc cgataagcct
     5281 gtcagataac gcaccgtgct gagctacctg ctggactggc tcggactgct ggtgcgctgg
     5341 ctgcacatcg ttgccggcat cgcctggatc ggcgcgtcgt tctatttcgt ctggctcgac
     5401 aatcatctgc aacccctgcg cgaggacggg ccagtcgatc cgaatgtagc cggcgaactc
     5461 tgggcggtgc acggcggggg tttctatcgt tcgcagaagt accgcgtcgc gccggcgcag
     5521 ctgccatcga ccctgcactg gttctactgg gaggcctaca ccacatggtg gtcgggcttc
     5581 gtgctgctgt gtctgctgta tttttttcgc gccgaggcgt acctcatcga tccgggggtc
     5641 gtggcgctgt ccaagccggt cgcgatcggc atcgcgctcg cggtgctggt cggcagttgg
     5701 ctgctgtacg atctgttgtg ccgctcacca ctgtcgcgtc acagcgttgc gctgagttcg
     5761 gcgctgggca ttgtgttcag cgcgatggcg tggggactgt gccatgtgtt cagcggtcgc
     5821 ggcgcttaca tgatcttcgg gacctcgatc ggcaccctca tggtggccaa tgtcgcgatg
     5881 gtgatcatcc cgggtcagcg cgagctggtg cgagccaggc agcaggcacg cgaggttgac
     5941 cccgcgcccg gtatgcgtgg caaacagcgc tcgctccaca acacctattt cacgctgccg
     6001 gtgctgttcg tgatgatcag caaccactat ccgatgacct acagccatcc gca
//
LOCUS       DATMRM010000277         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_482520, whole genome shotgun sequence.
ACCESSION   DATMRM010000277 DATMRM010000000
VERSION     DATMRM010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482520"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="VNZ06_08035"
     CDS             <1..1150
                     /locus_tag="VNZ06_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWW20738.1"
                     /translation="PIRAAWSLIVFPSLVLNYAGQSAIALRGVSVDDNIFFQLCPPSL
                     RIALVVLATLATIIASQSIITGAFSMTRQAILLGWLPRLQIKHTSEEGYGQIYVGAVN
                     WMLLVATVGLTLFFRKSDNLAAAYGIAVSLTMLMTSALLFIAMREVWKWNLLSSAAVA
                     GAFLCIDSVFFAANSLKILEGGYVPLLLAALVYGFMYIWHRGSLAVAASLHDSLLPVP
                     QFMEEVENKQIARVPGTAVFITRALREVPPVMLWHVRKNRCLHSKLLALTVVAESVPR
                     IRDGARLRITPLAPDFWRATAHYGFMERPDIPELLKQAPSLGCTLHLDDLTYYVGHET
                     VVAREDGKGLAKWEENLYSIMARNAMHVRDFFSLPSDHVVELGRQVSI"
     gene            1234..1998
                     /locus_tag="VNZ06_08040"
     CDS             1234..1998
                     /locus_tag="VNZ06_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWW20739.1"
                     /translation="MTLRLYTSWFSTFARKAALGLELKGLPYEAVDALIRPFHAELKR
                     VNARAEVPVLLDEDLVVVNSSDILQYLEWRYPQPVLYPQSISERVEARALERLADHRF
                     DPIVVDCSYWHWADRKDVAPPGLLDAGQTDIEAALTRLQQALATRPKPWPFESPGVVE
                     CAWFPNLIAVRPLGFSIDATRFPDVLGWLESMRRHPVFANDRQRTAEYLKTLPSRDIE
                     RGRLFFSGDRMEWLFSRGCHGWFHAEVAAGRTIFPD"
     gene            complement(2006..>3285)
                     /locus_tag="VNZ06_08045"
     CDS             complement(2006..>3285)
                     /locus_tag="VNZ06_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HWW20740.1"
                     /translation="GGMGEDCLVLNLWTPGLSSSAKKPVLFHIHGGGYYGGSGNSPGY
                     DGEALARFGDCVVITINHRLGAFGHLNLAQEGSQFARSGAVGMMDIVQALQWVRENVA
                     CFGGDPSRVLAFGQSGGGAKTSVLMAMPSAQGLYHRAGVMSGATLRLAEVEAAQKNTA
                     QLLSILGVQKGQAVKLQSLSFETILAAQATMEAGDRSRGEAPRSFAPTIDGVAIPGHP
                     FDPTAPAISADVPMIISNVIDERAYRKNNFDLDEAGLRAFIAHRIGEERASAVLKMYR
                     DEDPVATPFILQARFETDEVFRKSSLIQTERKAALHRASVWSYLWRVPSPAYGGRYGT
                     PHGSDVGPSLHDVRGALNGTSAESVRLADQIASAWVAFAATGNPNNPQTPNWPAYTLP
                     ARATLVFDHETRAESDPRGQFRQFWEKEPPQRG"
BASE COUNT          577 a         1076 c         1009 g          623 t
ORIGIN      
        1 gccgatccgc gccgcgtggt cgctgatcgt gtttccgagc ctggtgctca actacgccgg
       61 tcagtcagcg atcgcactgc gcggcgtctc ggtcgatgac aacatcttct tccagctctg
      121 cccgccgtcg ctgcgcattg cgctggtcgt gttggcaacc ctggcgacga tcatcgccag
      181 ccaatccatc atcaccggtg cgttctcgat gacgcgccag gcgatcctgc tgggctggct
      241 gccgcggctg cagatcaagc acacctccga ggagggttac gggcagatct atgtgggtgc
      301 cgtcaactgg atgctgctgg tcgcaaccgt cggtctcacg ctgtttttcc ggaaatccga
      361 caaccttgcc gccgcgtatg ggatcgccgt atcgcttacg atgctgatga cctcggcact
      421 gctgttcatc gccatgcgtg aagtctggaa gtggaacctg ctgagcagcg ccgcggtggc
      481 gggcgctttc ctgtgcatcg attcggtgtt ctttgcggcc aactcgctca agatcctcga
      541 gggcggctac gtaccgctgc tgctggcggc gttggtctac ggcttcatgt acatctggca
      601 tcgcggatca ctggcggtcg ccgcctcgct gcacgactcg ctgctaccgg taccgcagtt
      661 catggaggag gtcgagaaca agcagatcgc gcgcgttccg ggaaccgcgg tgttcatcac
      721 gcgcgcactc agggaggtgc cgccggtcat gctgtggcac gtccgcaaga atcgttgcct
      781 gcatagcaag ctgctggcgc tcaccgtggt cgctgagtcc gtgccgcgga tcagagacgg
      841 agcgcgactt cggatcacgc cgctggcgcc ggatttttgg cgggccaccg cccactatgg
      901 cttcatggag cggcccgaca ttccggaact gctgaagcag gcgccctcgt tgggatgcac
      961 gctgcacctg gatgatctga cctattacgt cggtcacgag accgtcgtgg ctcgcgagga
     1021 tggcaaaggt ctggccaagt gggaggagaa cctgtactcg atcatggcca gaaacgcgat
     1081 gcatgtgcgc gatttcttca gtctgcccag cgatcacgtg gtggaactcg gcaggcaggt
     1141 atcgatctga caggcctggg gaggagttga ctggacggag cggtctacaa ggaatttgac
     1201 cgcaccccca cggtcggtgc gacgatgtca cgcatgacgc tacgactgta caccagctgg
     1261 ttcagcacct tcgcgcgcaa ggcggccctc ggcctggagc tcaagggcct gccctacgag
     1321 gcggtcgacg cgctgatccg tccgtttcat gcggaactca aacgcgtcaa tgcgcgcgct
     1381 gaggtgccgg tactgctcga cgaggatctg gtggtggtca attcaagcga cattctgcaa
     1441 tacctggaat ggcgctaccc gcagcccgtg ttatatccgc agtccatttc agagcgtgtc
     1501 gaagcacgcg cgctggagcg cctggccgat caccgctttg atcccatcgt cgtggactgc
     1561 tcgtattggc actgggccga ccgcaaagac gtcgcaccgc ccggactgct cgatgccggt
     1621 cagacggaca ttgaggcggc gctgacgagg ttgcagcagg cgctcgccac ccgccccaag
     1681 ccctggccgt tcgaatcccc gggcgtggtc gagtgcgcct ggtttccaaa tctgatcgcc
     1741 gtacgacctc tcggattcag catcgatgca acccgcttcc cggacgtgct cggctggctg
     1801 gaaagcatga gaagacatcc ggtgtttgca aacgaccgcc aacgcaccgc cgagtatctg
     1861 aagacacttc cgtccaggga catcgagcgc ggcaggctct tcttcagcgg ggatcgcatg
     1921 gaatggctgt tctcgcgcgg ctgccacggc tggtttcacg ccgaagtcgc ggccggccgt
     1981 acgatctttc ccgactagct gcccgtcaac cgcgctgcgg cggctccttt tcccagaact
     2041 gcctgaactg gccgcgcgga tcgctctcgg cgcgcgtctc gtgatcgaac accagcgtcg
     2101 cgcgtgcagg cagtgtgtac gccggccagt tgggagtctg cgggttgttc ggattgccgg
     2161 tggcagcaaa cgccacccac gcggaggcga tctgatcggc caatcgcaca ctctcggcac
     2221 tggtgccatt cagagcgccg cgcacgtcgt gcagactcgg accgacatcc gagccgtgcg
     2281 gcgttccata gcgccctccg taggccgggc tcggtacgcg ccacagatag ctccagaccg
     2341 aagccctgtg aagtgcggcc ttgcgctcgg tctggatcaa tgacgacttg cgaaacacct
     2401 cgtcggtctc gaagcgtgcc tgcaggatga aaggcgtcgc caccggatcc tcatcgcgat
     2461 acatcttcag caccgcactg gcgcgctcct cgccgattcg atgcgcgatg aaggcgcgca
     2521 gacccgcctc atccaggtcg aaattgttct tgcggtaggc gcgttcatcg atcacgttcg
     2581 agatgatcat cggaacatcg gcggaaatcg ccggagcggt cggatcaaac ggatgtccgg
     2641 gtatcgccac cccgtcgatc gtcggggcga acgagcgcgg tgcctcgccg cgcgagcggt
     2701 caccggcttc catggtcgcc tgggccgcca gtatcgtctc gaacgacagg ctctgcagtt
     2761 tcaccgcctg acctttctgc acgccgagta tcgacagcag ttgcgcggtg ttcttctgcg
     2821 ccgcttccac ctccgccagc cgcaaggtcg cgccgctcat caccccggct cgatgataga
     2881 gcccctgagc cgatggcatc gccatcagaa cgctggtctt ggcaccaccc cctgactgac
     2941 cgaacgccag cacacgcgac ggatcaccgc caaagcaggc cacgttctcg cgcacccact
     3001 gcagggcctg cacgatgtcc atcatgccca cagcacccga acgcgcgaac tgcgagccct
     3061 cctgcgccag attcagatgg ccgaacgcgc ccaggcgatg gttgatcgtg atgaccacgc
     3121 agtccccaaa acgagcgagc gcctcaccgt catagcccgg agagttaccc gagccgccgt
     3181 agtagccgcc gccgtggata tggaacagga ccggtttttt cgcactggag ctcaggccag
     3241 gcgtccacag attcagtacc aggcagtctt cacccatgcc gcccg
//
LOCUS       DATMRM010000278         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1403714, whole genome shotgun sequence.
ACCESSION   DATMRM010000278 DATMRM010000000
VERSION     DATMRM010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403714"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..787)
                     /gene="modB"
                     /locus_tag="VNZ06_08050"
     CDS             complement(89..787)
                     /gene="modB"
                     /locus_tag="VNZ06_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HWW20741.1"
                     /translation="MTANFFSAGDLAALWISLKLAFITVACLLVLGTPLAWWLASGRS
                     RLRVVIEAITALPLVLPPTVLGFYLLLLLGPYGWGGRLMLWLGLSPLSFSFGGLLIGS
                     ILYSMPFVVQPLQNSFQAVGTRTLEVAATLRAAALDRFVSVALPLARRGFVTGAVLAF
                     AHTLGEFGVVLMIGGNIPGRTQTAAIAIFNHVEVLDYVAAQRLSLTLLAVCFILLLIV
                     YATNRGLRRDWVSV"
     gene            complement(784..1572)
                     /gene="modA"
                     /locus_tag="VNZ06_08055"
     CDS             complement(784..1572)
                     /gene="modA"
                     /locus_tag="VNZ06_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWW20742.1"
                     /translation="MTDLRTRRRVSWRWLCLWLAWLPAAADELNVAVAANFLSTLQQL
                     APKFQQASGHRLVISSGSTGQLYAQIKQGAPFDLFLSADGERPRLLESEGLGVPGSRA
                     TYAIGTLVLWSPRPEFVDANGKVLQSAQVRMIAIADPKNAPYGAAAQQVLTARGVWDE
                     FNRDHKIAMAENINQAWQFAATGNAQLAFIAKSQLSTLSQDGKPVTGSSWEPPQSLYT
                     EIDQDGLVLTRSTKQAAAESFMRWLRADTQAIAVITAAGYRVRE"
     gene            complement(1565..2377)
                     /locus_tag="VNZ06_08060"
     CDS             complement(1565..2377)
                     /locus_tag="VNZ06_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HWW20743.1"
                     /translation="MPRLRQPSLAVQGGLWLDRKGKTLVGADRIELLEAIDRLGSITH
                     AAKAVGLSYKAAWDSVDAMNNLAEHALLVRATGGAHGGGSHLTEHGRELVRLYRLLES
                     GYQRLLTQMQAQVHDFPKLNELMKAITMRTSARNQYRGKVATVRKGAVNSDVVLDLGD
                     GLTVSANITNDAVEDLALKPGRDAVALIKASFVLLTPDADTRVSARNKLRGTISQISP
                     GAVNAEVKLRLAGARVLTAVITLDALAELNLVVGTPCTALVKASHVLIAVND"
     gene            complement(2383..2922)
                     /locus_tag="VNZ06_08065"
     CDS             complement(2383..2922)
                     /locus_tag="VNZ06_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20744.1"
                     /translation="MKSLLPGLALTAFSIAALAQQAQAPDPCSAFSWNVTHERELFAA
                     HATAGTTGTNRAAAPLLDPDRLYELQLNLLAQVQFAAPPGKSKGGNGPYAGLALIRVP
                     KAGLWRIALDQPAWIDVVADGQPIASNDYQGRAGCHAPHKIVQFQLPASQPLIIQLSG
                     TPELQVRLTITRGEDSPKP"
     gene            complement(2964..>3295)
                     /locus_tag="VNZ06_08070"
     CDS             complement(2964..>3295)
                     /locus_tag="VNZ06_08070"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF013857.4"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HWW20745.1"
                     /translation="ALGSITAAVEAARTTAPGLRVEVETETLDQVREALEARADIIML
                     DDFDNETMRAAVAMNRSHPHRASLESSGGVRLERLRSIAETGVDFISIGSLTKNIEAV
                     DLSMRFD"
BASE COUNT          624 a         1049 c         1046 g          576 t
ORIGIN      
        1 aacagcgccg tgatgccaga gccgggaatg tccagctgca cctgcagccg gaagttccgg
       61 cgtgccatct cgacattcag cgacagactc ataccgagac ccagtcgcga cgcaggccgc
      121 gattggtggc atacacgatc agcagcagga tgaagcagac cgccagcagc gtgagcgaca
      181 ggcgttgcgc cgcgacgtag tcgagcactt caacgtgatt gaagattgcg atcgcagcgg
      241 tctgggtccg accgggaatg tttccaccga tcatcagcac cacgccaaat tcgccgaggg
      301 tgtgcgcgaa ggccagcacc gcgccggtga cgaagccgcg gcgcgccagc ggcagcgcga
      361 cgctcacgaa tcggtcgagc gctgcggcgc gcagggtcgc agctacttcg agggtgcggg
      421 tgccgaccgc ctgaaacgag ttttgcagcg gctgcaccac gaacggcatc gaatagagga
      481 tcgacccgat cagcagaccg ccgaagctga agctcagcgg cgacagtccc agccacagca
      541 tcaaccgacc gccccagccg tacggcccga gcagcagcag cagatagaaa cctaacacgg
      601 ttggtggcag taccagcggc agcgccgtga tcgcttcgat cacgacccgc agtcgcgagc
      661 gcccgctggc gagccaccac gcgagcggtg tgccgagcac cagcagacac gccaccgtaa
      721 tgaaggccag tttcagtgag atccaaagcg ccgccaggtc gccggcggaa aagaaattcg
      781 cagtcattcc ctgacacgat agccggccgc ggtgatcacg gcgattgcct gagtgtccgc
      841 tcgcaaccag cgcatgaagc tctcggcggc ggcctgcttg gtcgagcgcg tcagaacgag
      901 accgtcctga tcgatctcgg tgtagagcga ttgcggcggc tcccacgagg agccggtgac
      961 gggtttgccg tcctggctca gcgtgctgag ttgggacttg gcaatgaatg ccaactgcgc
     1021 gttgccggtc gcagcgaact gccaggcctg gttgatgttc tctgccatcg cgattttatg
     1081 atcgcgattg aactcgtccc acacgccgcg cgccgtgagt acctgctggg cggcggcccc
     1141 gtacggcgca ttcttgggat ccgcgatcgc gatcattcgc acctgagcgc tctgcaggac
     1201 tttgccattc gcatccacga attcaggacg cgggctccac agcaccagcg taccgatagc
     1261 gtacgtggcg cgactaccgg ggacgccaag accttcggac tccagcagtc tgggccgctc
     1321 accgtcggca gacagaaaca agtcgaacgg cgcaccctgt ttgatctgtg cgtagagctg
     1381 tccggtggat ccagaactga tcaccagccg atggccgctg gcctgctgaa attttggcgc
     1441 cagctgttgc agggtgctca ggaaattggc cgcgaccgcg acgttgagtt catccgctgc
     1501 ggccggcagc caggccagcc acagacacaa ccatcgccac gatacgcggc gccttgtgcg
     1561 caagtcagtc attgaccgcg atcagcacgt gagaggcctt gacgagcgcg gtacagggcg
     1621 taccgacgac cagattgagc tctgccagcg cgtcgagtgt gatgaccgcc gtgagtacgc
     1681 gtgcaccggc cagtctcagc ttgacctcgg cattgacggc accggggctg atctgcgaaa
     1741 tcgtgccgcg cagcttgttg cgcgcgctca cccgggtatc ggcatccggg gtcagcagga
     1801 caaaactcgc cttgatgagg gccaccgcat cgcggcccgg tttgagcgcc agatcttcaa
     1861 ccgcatcgtt ggtgatgttg gcgctcacag tcaggccatc acccagatcg agcacgacat
     1921 cggagttgac cgcgcctttg cgaacggtgg cgaccttgcc gcgatattga ttgcgggcac
     1981 tggttctcat ggtgatggcc ttcatcagtt cattcagttt ggggaaatcg tgtacctgag
     2041 cctgcatctg cgtcagcagc cgctgatagc ctgattccag tagccggtag agtcgcacca
     2101 gttcccgtcc gtgctcagtc aggtgactgc cgccgccatg cgctccaccg gtcgcgcgca
     2161 ccagcagcgc atgctccgcc aggttgttca tggcgtccac actgtcccat gcggctttgt
     2221 agctcaggcc gaccgctttg gccgcgtggg tgatcgaacc caggcgatcg atcgcttcca
     2281 gcagctcgat gcgatcggca cccaccaggg tcttgccctt gcggtcaagc cacagtccgc
     2341 cttgcaccgc cagggatggc tgcctgagtc tgggcatcgt tcttacggct tgggcgagtc
     2401 ctcgccgcgg gtaatggtca ggcgcacctg caactcggga gtgccgctga gctgaatgat
     2461 gagtggctgg gacgcgggca actgaaactg cacgatcttg tgaggtgcat gacacccggc
     2521 tcggccctga tagtcgttcg aggcgatcgg ctgcccatcg gcgaccacgt cgatccaagc
     2581 cggctgatcc agcgcaatgc gccacagacc ggctttgggt acgcgaatga gcgcgagccc
     2641 ggcgtaaggc ccgttaccgc ctttcgactt acccggcggc gcagcgaact gcacctgcgc
     2701 cagcagattc aactgcaatt catacagacg gtcgggatcc agcagcggcg ctgcggcccg
     2761 attggtgccg gtggtacccg ccgtggcgtg ggccgcgaac agctcacgct catgcgtgac
     2821 attccatgag aaagccgagc acggatccgg cgcctgcgcc tgctgggcaa gagcggcaat
     2881 gctgaatgcc gtcagcgcaa gcccaggcag caatgatttc atgatcgctg tataccgcgg
     2941 catatggccc ccggtaaagc gcgctagtcg aagcgcatgg acagatcgac ggcctcgata
     3001 ttcttggtca atgagccgat cgaaatgaag tccacaccgg tttcggcgat gctgcgcaac
     3061 cgctcgagcc tgacaccgcc ggaactctcg agcgatgcgc gatgggggtg ggagcggttc
     3121 attgccaccg cggctcgcat cgtctcgttg tcgaagtcgt cgagcatgat gatgtcggcg
     3181 cgggcctcga gcgcttccct cacctgatcg agcgtctcgg tctccacttc gacgcgcagg
     3241 cctggggccg tggtgcgcgc cgcctcgacg gccgcggtaa tggagccgag cgcgg
//
LOCUS       DATMRM010000279         9295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2324410, whole genome shotgun sequence.
ACCESSION   DATMRM010000279 DATMRM010000000
VERSION     DATMRM010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9295
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2324410"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VNZ06_08075"
     CDS             complement(<1..684)
                     /locus_tag="VNZ06_08075"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWW20746.1"
                     /translation="MFRRTGLILAVALMLTQAVSASRAAAPAPSLHLGYSDWPGWVAW
                     QIAIDKGWFKEAGLDVKFEWFDYSASMEAFAAGKLDGDFVTNGDALVMGAGGARNVTV
                     LVTDFSNGNDMIVARPGIHSLKDLRGRKIGIEVGLVEHLLLLDGLKKSAMTQEDVTLV
                     NSKTNETPQVLASGQVDAIGAWQPNSGLAIRALPGAHPVYTSAQSPGLIYDVLAVNPA
                     TLTTHREDYG"
     gene            896..1621
                     /locus_tag="VNZ06_08080"
     CDS             896..1621
                     /locus_tag="VNZ06_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP1"
                     /protein_id="HWW20747.1"
                     /translation="MTSSSSTELWNVRVEAGEHWSGILRRGTMLRITALQARANVAAL
                     MYNFELLSERYNMPDTLKAQHTAFLTSGCVCYSDMGRILCSITADTCGWHDTVCGLSD
                     AAMVAEKHGPSRFQEQRNAAHKNGRDSMLNELGKYGLGKRDLVANINFFSRITADEDG
                     GLHFDPEHCRAGAFVDLRFEMHTLLVLATCPHPLDPDTSYRPRPVSLSATRAAQVAPD
                     DPCRMRCPENARGFQNTERAFLT"
     gene            1618..2286
                     /locus_tag="VNZ06_08085"
     CDS             1618..2286
                     /locus_tag="VNZ06_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005404253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase associated protein UAAP2"
                     /protein_id="HWW20748.1"
                     /translation="MNLSATDAINDEIVAAGEPWLGAVEKGQILRIVDLEGNQAVDTL
                     FFNLHDPQERYSAVDTIRAQGKLYLSTGTELRSNLDSTMLTIIEDTCGRHDTLGGACS
                     AESNTVRYAHEKRHMHSCRDNFLLALSTYDQKLTKRDLPPNINFFMNVPVTATGGLEF
                     ADGISAPGRYVQMRANMDVLVLISNCPQLNNPCNAFNPTPIQIMIWEAGANIPSDRET
                     TRGS"
     gene            2326..5994
                     /gene="uca"
                     /locus_tag="VNZ06_08090"
     CDS             2326..5994
                     /gene="uca"
                     /locus_tag="VNZ06_08090"
                     /EC_number="6.3.4.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004847 - urea carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase"
                     /protein_id="HWW20749.1"
                     /translation="MFDKVLIANRGAIACRIQRTLRRMGIGAVAVYSEADQASLHVRH
                     ADEAILIGTGPAADSYLLTERILEAARHSGAQAIHPGYGFLSENAQFAESCERAGIAF
                     IGPTAQQIRNFGLKHTARALALAQSLPLLPGSGLLNDLPHATREAERIGYPVMLKSTA
                     GGGGIGMQLCANAAELAAAFDSVRRLAASNFRDGGVFLEKYVSRARHIEVQLFGDGRG
                     NVIHLGERDCSMQRRNQKLIEETPAPGLPDALRHELWQSAVRVGTAVGYRSAGTVEFI
                     IDSDRIESFPGDAFYFLEVNTRLQVEHGVTEAVTGIDLVEWMIRVAADDPPALRDYRH
                     APSGSAIQARVYAEDPARAFRPSSGLLTEVRWPQTLRVDTWVDTGCEVPAFYDPMLAK
                     IISHAPARAEAIARLDAALADSSVQGIETNLDYLRALLAGEEFRTATHTTRLLSSLPF
                     ASRTIEVLLPGTHTTLQDLPGRLNFWDVGVPPSGPMDPLAFALANRLLENDTDCAALE
                     LTVSGPTLKFASDTMIALTGAPMPAELNGTAVEPWRATRVSAGSVLRVGAIEGAIGQR
                     AYLAVLGGFDVPRYMGSRSTFTLGQFGGHAGRALRNGDVLRLAAAGQSDADTASRLRA
                     LTVPAQLHPAYTHDWELRVLYGPHAAPDFFTEGDIETLFATAWQVHYNSSRTGVRLIG
                     PKPTWARRDGGEAGLHPSNIHDNAYAVGAIDFTGDMPVILGPDGPSLGGFVCPATIAL
                     VDLWKVGQVRPGDRVRFRPVSAPQAQAALRAQETAIRTLRNPERTGAPHRHACCESRQ
                     AILEQRAADAARPAVCYRQSGEDNVLIEFGEPTLDLALRFRVQAMLDALHARAIPEVI
                     DLTPGVRSLQVHFDCRHTSRDQIMRTLLEVEQQLASVDDLEVASRIVHLPLSWDDPAT
                     RLAIERYAQSVRADAPWCPSNIEFIRRINGLDSIEQVRRIVFDASYLVMGLGDVYLGA
                     PVATPLDPRHRLVTTKYNPARTWTPENAVGIGGAYLCVYGMEGPGGYQFVGRTVQMWN
                     RYRQTRDFREGKRWLLRFFDQLRFHSVSAEELLRLRAEFPLGKGELQIEPTTLRLRDY
                     QQFLAANAASIAAFKARQQAAFDAERERWRAMGRESDNPPEAPDPNASNAALEAIPEG
                     CVAISSPVTGSVWQLKARVGERAAAGASLIVIESMKMEIAVQSEHSGTIVELRCQRGQ
                     PVTAGETLLVLRPASWTP"
     gene            5991..7817
                     /gene="atzF"
                     /locus_tag="VNZ06_08095"
     CDS             5991..7817
                     /gene="atzF"
                     /locus_tag="VNZ06_08095"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HWW20750.1"
                     /translation="MNLPALTCARSLGIADLSAAYRDRRTTPAGLVRELYARIATCPD
                     PAVWISLLPIEQVLEQAQVLADCDPASLPLYGVPFAIKDNIDLADVRTTAACEAFAYL
                     PEQSAQVVTRLIQAGAMPIGKTNLDQLATGLVGTRSPYGICHSSFNANYISGGSSSGS
                     AVAVAHGLVSFALGTDTAGSGRVPAAFNNLIGWKPTCGRLSPAGLVPACRTADTISVL
                     ALTAEDAARVGRVAEGFDPAQPYSRRAHPCPGAAALTRALFRFGVPRAEQLQFFGNDE
                     YPRLFDAAVAHMERLGAQRVLIDFTPFLEAGQLLYGGPWVAERYLVAESILRRSPQSL
                     LPVTRQIIERGSEPSAADAFRAQYQLQGFKRAAELAFDSIDVLLTPTVPTHYRIDEIE
                     ADPIRLNSRLGYYTTFMNLLDMAAVAVPAGFTTTGLPFGVTLAAPAWSDMALLSLASR
                     LHRASPSKLGATQIELAAEQHFDWSDLSSAIPVAVCGAHMEGLPLNHQLMQRGAVLLE
                     RTRTAASYRLYTLPGGPPYRPGLIAVDSHGAAIEVEVWSVPAHAFGSFVAGIPSPLGI
                     GKITLGDRRQVSGFLCEAHALSSAQDITSFGGWRAFIASRSP"
     gene            complement(7814..8527)
                     /locus_tag="VNZ06_08100"
     CDS             complement(7814..8527)
                     /locus_tag="VNZ06_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HWW20751.1"
                     /translation="MRRILVSLLATALTGAASAASSPNWAQGIHYFLIQPAHPTSPTS
                     VSAGKVEVTEVFSYACPACDRFYPTVDKLRAALPVNAELTFVPAAFNQAEDWPMFQRA
                     FYAAQSLGIDKKTHDAVFDAVWKTGELAVVDPGSERLKVPAPTIQDAANWYARAAGVT
                     PQTFVNAASSFGVDTKTRQADEFIRKTQVDQTPTMIVNGKYRVTVGSACAGEPRCADS
                     EQRIIDVVLFLVKQESGAH"
     gene            8670..>9295
                     /locus_tag="VNZ06_08105"
     CDS             8670..>9295
                     /locus_tag="VNZ06_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWW20752.1"
                     /translation="MTATWDPVLLARAQWSFTMAFHIVFPAISIGLASYLAVLEGLWL
                     TTGREAYLNLFRYWLKIFAVAFGMGVVSGLVMAYQFGSNWSAFSDKAGPVIGPLMAYE
                     VLTAFFLEAGFLGVMLFGLERVGKSLHFSATLAVAFGTLTSAFWILATNSWMQTPAGF
                     TTNAVGQFIPVSWRQIIFNPSFPYRLVHMVLASYLSTALIVGAVAAWHL"
BASE COUNT         1621 a         3020 c         2911 g         1743 t
ORIGIN      
        1 gccgtagtcc tcgcgatggg ttgtgagcgt cgccggattg accgccagca cgtcatagat
       61 caatcctgga gactgcgccg aggtgtagac cgggtgcgcc cccggcagcg cgcggatcgc
      121 gagccccgag ttcggctgcc aggcgccgat tgcatcgacc tgacccgagg ccagcacctg
      181 gggtgtctcg ttggtcttgg aattcaccag cgtgacatcc tcctgggtca tcgccgattt
      241 cttcaggcca tcgagcaaca gcaggtgctc caccagcccg acctcgatgc cgatctttct
      301 gcccctgaga tcctttagcg agtgaatacc gggacgcgca acgatcatgt cgttgccgtt
      361 ggagaaatcc gtcaccagca ccgtgacgtt gcgggcgccg cccgcgccca ttaccagcgc
      421 atcgccgttg gtcacgaagt cgccatccag cttgccggcc gcaaaggcct ccatcgacgc
      481 cgagtagtcg aaccactcga atttcacgtc gagtccggcc tccttgaacc agcccttgtc
      541 gatggcaatc tgccaggcca cccaccccgg ccagtcgctg tacccaagat gcagcgacgg
      601 cgctggggcc gccgcccgcg atgccgagac agcctgggtc agcatcaagg ctactgcgag
      661 gatcaacccg gttcttcgaa acatcgtcat ttctcgcgcg ctgtgttatt acgaattcac
      721 gcttccgtat taccatcctg ctgcaagcgc tatgcctcga atttcccgcc cggttcgcga
      781 ccacaggaag ctgcgccgca ccgctttagt gccagcgagc ccattcacgt tcgattgtgg
      841 ggcgtgtgaa gggctttatt tgttggcgcg cttgccttaa gtttttccac cacccatgac
      901 gagcagctca tccacagaac tctggaacgt tcgagtcgag gcgggcgagc attggtccgg
      961 catcctgcga cgcggcacga tgctgcgaat caccgcgctg caagcgcgcg ccaatgttgc
     1021 cgcgctgatg tacaacttcg aactgctgtc cgagcgctac aacatgccgg atacgctgaa
     1081 agcgcagcac accgcatttc tgacctcagg ctgcgtgtgc tattcggaca tggggcgcat
     1141 tctgtgctca atcacggctg acacttgcgg ctggcacgac accgtctgcg gtctgtccga
     1201 tgccgcgatg gtggcggaga aacacgggcc atcgcgcttt caggaacaac gcaatgctgc
     1261 gcacaagaac ggtcgcgaca gcatgctcaa tgaactcggt aaatacggac tgggcaaacg
     1321 cgatttggtg gccaacatca atttcttcag ccgcatcacc gctgatgagg acggtgggct
     1381 gcacttcgat ccggaacatt gtcgggctgg cgccttcgtg gacctgcgct tcgaaatgca
     1441 cacgctgctg gtgctggcaa cctgcccgca tccgctcgat ccggacacga gctatcgacc
     1501 gcgcccggtg tcgctgagtg cgactcgcgc agcgcaggtc gcgccggacg atccgtgccg
     1561 gatgcgctgc cccgagaatg cgcgcggctt ccagaacact gaaagagcat ttctcacatg
     1621 aacctgtccg ccacggatgc gatcaacgat gaaatcgtcg ccgccggcga gccgtggctc
     1681 ggcgcggtgg aaaagggaca aatcctgcgc atcgtcgatc tggaaggcaa ccaggccgtc
     1741 gatacgctgt tcttcaatct gcatgacccg caggagcgct atagcgcggt cgataccatc
     1801 cgcgcgcagg gcaagctgta cctgagcacc ggcaccgagc tgcgctcgaa tctggattcg
     1861 accatgctga cgatcatcga ggatacctgc gggcgtcacg acacgcttgg gggcgcctgc
     1921 tcggccgaga gcaacacggt ccgctacgcg cacgagaagc ggcacatgca cagttgtcgc
     1981 gacaatttcc tgctggcact gagcacctat gaccagaagc tcaccaagcg cgatctgccg
     2041 cccaacatca atttcttcat gaacgtgccg gtcaccgcca cgggtggcct cgaatttgca
     2101 gatggaatct cggcgccagg gcggtatgtg cagatgcggg cgaatatgga tgtgctggta
     2161 ttgatctcca actgcccgca actgaacaat ccctgtaacg ccttcaatcc aacgccgata
     2221 caaatcatga tctgggaggc gggggcgaac atcccgtcgg accgcgagac gactcgcggc
     2281 tcctgattgc ctgccgggac gacccggtgt tcgtgggagt tcgggatgtt cgacaaagtg
     2341 ctgatcgcca atcgcggcgc tatcgcgtgc cgcattcagc gcacgctgcg acggatgggc
     2401 atcggcgcgg tggccgtcta ctcggaggcc gaccaggcga gcctgcatgt ccgccatgcc
     2461 gatgaggcga ttctgattgg caccggtccg gccgccgaca gctatctgct taccgagcgc
     2521 attctggaag ccgcgcgcca cagcggcgcc caggccattc atcccggata cggtttcctc
     2581 agcgagaatg ctcagttcgc cgaaagctgc gagcgagccg gtatcgcctt catcgggccg
     2641 accgcgcagc agatccgcaa cttcggcctg aagcacacgg cacgcgcgct ggccctggcg
     2701 cagagtctgc cgctgctgcc cggaagcgga ctgctgaacg atctgccgca tgcaacgcgc
     2761 gaagccgaac gtatcggcta cccggtgatg ctcaagagta ccgccggcgg gggcggtatc
     2821 ggcatgcagc tgtgcgcgaa cgcggcggag cttgcggccg cgttcgatag cgtgcgccgc
     2881 ctggcggcga gcaattttcg cgatggcggt gtctttctcg aaaagtacgt gtcccgcgca
     2941 cgccatatcg aagtacaact gttcggcgac ggccgaggca acgtcattca tctgggtgag
     3001 cgcgactgct cgatgcagcg tcgcaatcag aagctgatcg aggaaacccc tgcgcccggc
     3061 ctgccggatg cgttgcggca cgagctgtgg cagtcggcgg tacgcgtggg aactgcggtc
     3121 ggctatcgct ccgcgggaac cgtggaattc atcatcgaca gcgaccggat tgagtcgttc
     3181 ccaggcgatg ctttctactt tctggaagtc aatacgcggc tccaggtcga gcatggtgtc
     3241 accgaggcgg tcaccggaat cgacctggtg gagtggatga ttcgcgtcgc ggccgacgat
     3301 cccccggcgc tgcgcgacta tcgtcacgcg ccgagcggca gcgccatcca ggcccgtgtc
     3361 tacgccgagg atcccgcacg cgcgtttcgc ccatccagcg gcctgctgac cgaagtgcgc
     3421 tggccgcaga ctttgcgggt ggatacctgg gtcgacacgg gctgcgaggt gccggcgttt
     3481 tacgatccga tgctggcgaa gatcatttcg catgccccgg cacgcgccga ggcgatcgca
     3541 cggctcgacg cggccctggc tgacagttca gtgcagggca ttgagaccaa cctcgactat
     3601 ctgcgcgcgc tgctggcggg cgaggaattt cgcaccgcga cgcacaccac gcgccttctg
     3661 agctcactgc cgttcgcttc gcgtacgatc gaagtactgc tgccgggtac acacaccacg
     3721 ctgcaggatc tgcccggaag gctcaacttc tgggacgtgg gagtaccacc ttccgggccg
     3781 atggatccgc tggcgttcgc gctcgcgaac cgcctgctcg agaacgacac cgactgcgcg
     3841 gcgctggaat tgaccgtcag tggtccgacg ctgaaattcg ccagcgacac gatgatcgca
     3901 ctgaccggtg cgccgatgcc ggccgaactc aacggcacag cggtcgaacc atggcgagcg
     3961 acccgcgtgt cggccggcag cgtgctgcga gtcggtgcga tcgagggcgc tatcggtcag
     4021 cgcgcctatc tggccgtgct cggcggcttc gatgtgccgc gctatatggg cagccgttcc
     4081 accttcacgc tcggtcagtt tggtggccat gccggacgcg cactgcgcaa cggcgacgta
     4141 ctgcggcttg ccgcggccgg acagagcgat gccgacacgg cgagccgctt gcgcgcactc
     4201 accgttcccg cgcagctaca tccggcgtac acgcacgact gggaactgcg agtgctctac
     4261 gggccgcatg cggcgccgga tttcttcact gagggtgaca tcgagacctt attcgcgacc
     4321 gcctggcagg tgcactacaa ctcgagccgc accggcgtac gtctgatcgg ccccaaaccc
     4381 acttgggcgc gccgggacgg cggtgaagcg ggcctgcatc catccaatat tcacgacaat
     4441 gcctatgcgg tgggcgcgat cgactttacc ggcgatatgc ccgtcatctt gggtcccgac
     4501 ggcccgagcc tgggaggctt cgtctgtccg gcaacgatcg cactggtgga cttgtggaag
     4561 gtcggacaag tcaggcccgg cgatcgggtg cgtttccgcc ccgtcagtgc gccgcaggcg
     4621 caggccgctc tgcgcgccca ggaaacggcg atccgcacgc tgcgcaaccc ggagcgaaca
     4681 ggcgcgccgc atcggcacgc ttgctgcgaa tcgcgccagg cgatcctcga gcagcgcgcc
     4741 gctgacgctg cccgcccggc ggtgtgctat cgccagtcgg gtgaggacaa tgtgctcatt
     4801 gagtttggcg aacctacact cgatctcgcc ttgcgctttc gcgttcaggc gatgcttgat
     4861 gctctgcacg cacgcgcgat tcccgaagtc atcgatctga ctcccggggt gcgctcactg
     4921 caggttcatt tcgactgtcg ccataccagc cgcgatcaga tcatgcgtac tttgctcgag
     4981 gtcgagcagc agctggcgtc ggtcgatgat ctggaggtcg cgagccgcat cgtgcatttg
     5041 ccgctgtcgt gggacgatcc tgcgacccgt ctcgcgatcg agcgctatgc gcagtcggtg
     5101 cgcgcggatg cgccctggtg tcccagcaac atcgagttca ttcgtcgtat caacggattg
     5161 gactcgatcg aacaggtgcg ccgcatcgtg ttcgatgcca gttatctggt gatgggtctc
     5221 ggcgacgtgt atctcggcgc accggtcgca accccgctcg atccgcgcca tcggctggtg
     5281 acgacgaaat acaatccggc gcgcacctgg acaccggaaa atgctgtcgg cattggtggc
     5341 gcgtatctgt gcgtctatgg catggagggt ccgggcggct accagtttgt cggacgcacg
     5401 gtacagatgt ggaaccgcta ccggcagacg cgcgactttc gtgagggcaa acgctggttg
     5461 ctgcgcttct tcgatcagct gcgcttccat tcggtgtccg cggaagagct gctgcggctg
     5521 cgcgcagagt tcccgctcgg caagggcgag ctgcaaatcg aaccgaccac gctgcggttg
     5581 cgcgactacc agcagttcct cgctgcgaat gccgcctcga tcgccgcctt caaagcgagg
     5641 caacaggccg cgttcgacgc cgaacgcgaa cgctggcgcg cgatgggccg tgagagcgac
     5701 aatccgcccg aagcacccga tccgaatgcc agcaatgcag cgctcgaagc gatccccgag
     5761 ggatgtgtgg caatcagttc gcccgttacc ggcagtgtgt ggcaactcaa ggcccgcgtc
     5821 ggggagcggg cagcggccgg ggcctcactg atcgtcatcg agtcgatgaa aatggaaatc
     5881 gcggtgcaaa gcgaacacag cggcaccatt gtcgagttgc gctgccagcg aggccagccg
     5941 gtcacggctg gcgagaccct gctggtattg agaccggcaa gctggacgcc atgaacttac
     6001 ctgcgctcac ctgcgcacgc agcctcggca tcgccgatct gagcgccgcc tatcgcgacc
     6061 gccggaccac gccggccggc ctggtcagag agctgtatgc tcgcatcgcc acctgccccg
     6121 atccggccgt atggatctcg ctgttgccca tcgagcaggt gctggagcag gcgcaggtgc
     6181 tggcggattg cgatccggcg tcactgccgt tgtacggcgt cccatttgcg atcaaggaca
     6241 acatcgacct cgccgacgtg cgcaccacgg ccgcctgcga ggcctttgcg tacctgcccg
     6301 aacagtcggc gcaggtggtc acgcggctca tccaggcggg cgccatgccg atcggcaaaa
     6361 ccaacctcga tcagctcgcc accggactgg tgggcacgcg ctcgccttac ggcatctgcc
     6421 atagcagttt caatgcgaac tatatcagtg gcggttcgag ttccggatcc gcggtggccg
     6481 tggcgcacgg cctggtcagc tttgcgctcg gcaccgatac cgccggctcc ggccgcgtgc
     6541 ccgccgcgtt caacaatctg atcggctgga agcccacctg cggacgcctg agtccggcgg
     6601 gactggtgcc cgcctgccgc acggctgaca cgatctcggt attggcgctc accgcggagg
     6661 atgccgcacg tgtgggccgc gtcgcggaag gtttcgaccc cgcgcaaccc tattcgcgcc
     6721 gtgcgcaccc ctgcccaggt gccgctgcac tgacacgcgc actgttccga tttggagtgc
     6781 cacgcgcgga gcagctgcag ttcttcggca acgatgaata tccgcggctg ttcgatgcgg
     6841 cggtcgcgca tatggagcgc ctgggcgctc agcgcgtgct gatcgatttc accccgtttc
     6901 tcgaggccgg ccagctgctg tatggaggtc cctgggtcgc ggagcgatat ctggtggcgg
     6961 agtcgatcct gcgtcgctcg ccgcaatcac tgctgccagt gacacgccag atcatcgagc
     7021 gcggaagtga gcccagtgca gccgatgcat tccgcgcgca gtatcagctg caggggttca
     7081 agcgcgccgc cgaactggcc tttgacagca tcgacgtgct gctgaccccg accgtgccca
     7141 cccactatcg catcgacgag atcgaagccg atccgatccg gctcaacagc cgtctgggct
     7201 actacactac tttcatgaac ctgctggaca tggctgcggt cgcagtaccc gccggattca
     7261 cgaccacggg actgccgttc ggcgtcacgc tggcagcgcc cgcgtggagc gacatggcgc
     7321 tgctgtcact ggcctcgcgg ctgcatcgcg cctcaccgtc caagctaggc gcgacccaga
     7381 tcgaactggc tgccgaacaa cacttcgact ggtcggacct cagcagtgcc atcccggtcg
     7441 ctgtctgcgg cgcccacatg gaggggctac cgctcaacca tcaactcatg cagcgcgggg
     7501 cagtgctgct cgagcgcacg cgaaccgcag cgagctaccg gctgtacacg ctacccggcg
     7561 gaccgcctta tcggcccggg ctgatcgccg ttgactcgca cggcgccgcc atcgaagtcg
     7621 aggtatggtc cgttcccgct catgccttcg gcagcttcgt cgctggcatt ccctcaccct
     7681 tagggatagg caagatcacc ctgggagatc gccgtcaggt cagcggcttt ctgtgcgaag
     7741 cgcatgcgct gagctcggcg caggacatca cgtctttcgg aggctggcgc gcgttcatcg
     7801 ccagccgcag cccctagtgt gcgccacttt cctgtttcac caggaacagc acgacgtcga
     7861 tgatcctctg ctcactgtcc gcgcagcgcg gctcgccggc gcaggctgag ccgacggtga
     7921 cgcggtattt gccgttcacg atcatggtgg gtgtctgatc cacctgggtc ttgcgaatga
     7981 attcgtcggc ctggcgcgtc ttggtgtcca cgccgaatga actggcggca ttgacgaaag
     8041 tctgcggggt cacgccagcg gccctcgcat accagttggc ggcgtcctga atcgttggcg
     8101 ccggaacctt gaggcgctcg ctgcccggat cgaccaccgc cagctcaccg gttttccaga
     8161 cggcatcgaa tacggcatcg tgggtcttct tgtcgatgcc gagtgactgc gccgcgtaaa
     8221 acgcacgctg aaacattggc cagtcctctg cctggttgaa ggccgccggc acgaaggtca
     8281 actcggcgtt caccggcagc gccgcgcgca gcttgtcaac cgtgggatag aagcgatcac
     8341 acgccgggca ggcataggaa aatacttccg tcacttcgac cttgcccgca ctcaccgacg
     8401 tcggtgatgt cgggtgcgcc ggctgtatca gaaaataatg gatgccctgg gcccagttcg
     8461 ggctggaagc cgcagacgcg gcgcccgtca gcgccgtggc cagcaaggac acaagaattc
     8521 gtcgcatgta cacaacaccc ctcttgacgg tctggcgaca agcatagctt gcaggctcac
     8581 cctatgacac tgcctccggg cggtcgttct gggcggccga aggaattctg tgcttcgcgg
     8641 cggaccggcg cgcgtggcac catgcgggta tgactgcgac atgggatcca gttctcctgg
     8701 cgcgagcgca atggtcattc acgatggcgt ttcacatcgt gtttccggct atttccatcg
     8761 gtctggcaag ctatctggcg gtacttgagg gcttgtggct cacgactggg cgcgaggcct
     8821 acctgaatct gttccggtac tggctgaaga tctttgcggt cgcgttcggc atgggcgttg
     8881 tctcggggct ggtgatggcc taccagttcg gcagcaactg gtccgccttc tccgacaagg
     8941 ccggcccggt gatcggaccg ctgatggcct atgaggtact caccgccttt tttctggagg
     9001 ccggcttcct cggcgtgatg ctgtttggcc tcgagcgcgt aggcaagtcc ctgcacttca
     9061 gcgccacact ggcggtggcg ttcggtacgc tgacttccgc gttctggatc ctggcgacca
     9121 acagctggat gcagaccccg gccggcttca ccaccaacgc ggtgggtcag ttcattccgg
     9181 tgtcgtggcg gcagatcatc ttcaatcctt cgtttcccta tcgccttgtt catatggtac
     9241 tggcgtcgta tctgagcacc gcattgatcg tgggcgccgt ggcggcctgg cacct
//
LOCUS       DATMRM010000280         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2325246, whole genome shotgun sequence.
ACCESSION   DATMRM010000280 DATMRM010000000
VERSION     DATMRM010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2325246"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="VNZ06_08110"
     CDS             <1..1109
                     /locus_tag="VNZ06_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HWW20753.1"
                     /translation="DPISVGSTVTWNRLRATVSGPPSTHVAFHYALANTATSPPVDRA
                     APDPFADPAWRPLPVDTTDFLLTGAKATHLFLGALFTSDQTATPQLAQIRVDFDTESF
                     TRYLPAIYRQPGSDFLERFVRLLQGMFEDIETEVDTLDRYFDPLAAPADALPWLATWL
                     AVDLDQGEAESRIRHSIAGAFHRYRWRGTVEGLRLALLEDAGVHAVITEPIAAASFWG
                     FTGDGGSAGAGGALGLSTHLPPAEPGGAVLGSTAELDHAYLITDDRFGEPLFEGAAWQ
                     FLIQVYRHEVGTDARLQLVKEIIERERPAHAMYRLELIDPTMQVGAQARVGIDAIISG
                     AAEPTPLGGGVGLHLAGLPPPRIGSSILGSDLKL"
     gene            1131..1898
                     /locus_tag="VNZ06_08115"
     CDS             1131..1898
                     /locus_tag="VNZ06_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20754.1"
                     /translation="MQTTLHALTPSDQVHTFVRNRYFYGKLLDVFHFELESDYLNGKR
                     WLLNRLISGYGVLCGLDVQVAPNGRAVVVTPGVALDRAGHEIIVPRITDPVPLPPRPA
                     TSTTTAANCEPEDFVHLCICFEQCEGDPTPVMVDNCGQAVTCASSSYQERYRCVIRDC
                     KAPDIQMVTGLPDLIMNGRLNYYALVERVTRGCPALPMDVCVPLANIRLPSGEDPPKA
                     SDIDISPRPIVYSNDLLFDMFLALGGDAVPRNRGGKQ"
     gene            1902..>2394
                     /locus_tag="VNZ06_08120"
     CDS             1902..>2394
                     /locus_tag="VNZ06_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20755.1"
                     /translation="MEDRMNITPMNMTPCHCADSGGLQTMSTNVGERPRYYARQLVMP
                     DDLTLEHEYFRAKMRRHNRFLHGWGVVCGARVVAAQQPWKVIVRTGYILGPYGDEICI
                     GKDQCIDVRTTCVTGQPQPPMDDCGCTEASPPIPADTAMFLAIRYVEKPSRLVRVPLG
                     GCGC"
BASE COUNT          421 a          814 c          731 g          428 t
ORIGIN      
        1 gcgacccgat atcggtcggt tcgaccgtca cctggaaccg gctccgggca accgtcagcg
       61 gcccgccttc cacccacgtg gccttccact acgccctcgc gaacacggca acgtccccac
      121 cagttgatcg agccgctccg gacccctttg cagacccggc gtggcgaccg ctgccggtcg
      181 ataccaccga cttcctgctg actggcgcga aagcaactca cctcttctta ggcgccctgt
      241 tcaccagtga ccagaccgcc acgccacaac tggcccagat ccgggtcgac tttgacaccg
      301 agagcttcac ccgctacctg ccggcgatct accgccagcc cggttccgac tttcttgaac
      361 gcttcgtccg cctgctccag gggatgttcg aggatatcga gaccgaagtc gacacgctgg
      421 atcgctattt cgatccgctg gccgcgccag ccgatgcctt gccatggctg gcgacgtggc
      481 tcgcggtgga tctggaccag ggcgaggcgg agagtcgaat ccgccacagc atagccggcg
      541 cattccaccg ttatcgctgg cgcggcaccg ttgagggact gcggctggca ctgctcgagg
      601 atgccggcgt gcatgcggtg atcaccgagc ccatcgccgc tgcgtcgttc tggggattta
      661 ccggcgacgg cggcagcgcc ggcgcggggg gcgcgcttgg gctgagtacc catctgccac
      721 cggcagagcc tggcggcgcc gtactcggca gcacggccga actcgaccac gcctacctca
      781 tcacggacga ccgtttcggc gagcctctgt tcgaaggcgc ggcctggcaa ttcctgatcc
      841 aggtctatcg ccatgaggtt ggcaccgacg caaggctgca gttggtgaag gagatcatcg
      901 agcgcgagcg gccggctcac gcgatgtatc ggctggagct gatcgatccg acgatgcagg
      961 tcggggcgca ggcacgggtg ggcatcgacg cgatcatttc cggagcagcc gaaccgacgc
     1021 cccttggcgg tggcgtcggc ctccacctgg ctgggcttcc gccgccccgg atcggcagca
     1081 gcatcctggg cagcgacctg aaactctgac tctcgacgcg aggacgcacc atgcaaacga
     1141 ctcttcacgc cctgacgcca tcggaccaag tccacacctt cgttcgcaac cggtatttct
     1201 atggaaaact cctcgatgtt ttccatttcg agctggagtc cgattacctc aacggcaagc
     1261 gctggctgct gaatcggctg atcagcggat acggtgtgct gtgcggcctc gacgtgcagg
     1321 ttgcaccgaa cgggcgcgcc gtggtcgtca cgccaggcgt cgcgcttgac cgcgccgggc
     1381 atgaaatcat cgtgccgcgt atcaccgatc cggtgcccct gccgccgcga ccggccacct
     1441 cgaccaccac cgcagcaaac tgtgaaccgg aagactttgt gcatctctgc atctgcttcg
     1501 agcagtgcga gggcgatccc acgccggtca tggtggacaa ctgcgggcag gcggtcacct
     1561 gcgcctcctc gtcatatcag gagcgctacc ggtgcgtcat ccgcgactgc aaggcgcccg
     1621 acatccagat ggttaccggg ctgcctgacc tgatcatgaa cggccggctg aactactatg
     1681 ccctggtcga gcgggtgaca cgcggatgcc cggcgctgcc gatggatgtc tgcgtcccac
     1741 tcgccaatat ccgcctgccc tcaggcgaag atccgccgaa ggcgtctgat atcgacatca
     1801 gcccgcgccc gatcgtgtac tcgaacgacc tgctgttcga catgttcctg gctctcggcg
     1861 gcgacgcagt accgcgcaat cgcggcggca aacagtagaa gatggaggac cgcatgaaca
     1921 tcacccccat gaacatgacc ccgtgtcact gcgccgattc cggcggcctg cagacgatgt
     1981 cgacgaatgt cggcgagcgg ccgcgctact atgcgcgtca gctcgtgatg ccggacgatc
     2041 tgacgcttga gcacgaatat tttcgcgcca agatgcgccg ccacaaccgt ttcctgcatg
     2101 gctggggcgt agtttgcggt gcccgcgtgg tggccgcgca gcagccgtgg aaggtgatcg
     2161 tcaggaccgg ctacatcctt ggcccgtatg gtgacgagat ctgcatcggc aaagaccagt
     2221 gcatcgatgt ccgcaccacc tgcgtcaccg gacagccgca accaccgatg gatgactgcg
     2281 gctgcaccga ggcttctcca ccgatacctg ccgacaccgc gatgttcctg gcaatccgct
     2341 atgtcgaaaa accgagccgt ttggtgcgtg tacctctcgg cggctgtggc tgcg
//
LOCUS       DATMRM010000281         3765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_146993, whole genome shotgun sequence.
ACCESSION   DATMRM010000281 DATMRM010000000
VERSION     DATMRM010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146993"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1616)
                     /gene="fliF"
                     /locus_tag="VNZ06_08125"
     CDS             complement(111..1616)
                     /gene="fliF"
                     /locus_tag="VNZ06_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HWW20756.1"
                     /translation="MASTREKQLVSWSRLPTFGRAGMILGAALIVAGAVALAVWSSST
                     DYQVLFAQLGEADAANVVEQLKHQKIPYRLADQGTTVKVPAGQVYETRLALVSSGLPL
                     SGGVGFEIFDREGLGASEQSQRIAYQRALQGELARTISALQGVQSARVHLVLPESTMF
                     KRDREEPRAAVSLVLKAGASLSAEQTAGVQRLVAASVSNLDASKVVITDQRGITLSGG
                     DGTEGGTTGSDARLSIKRSIEDYIARKVGTLLDSAYGSGQALVSVDVALNFDQIKSTV
                     QDLVPMKGTPAGPEGAVLRRRQVESGAGPTPAVTSESATAGYAKPSGSTTEVEYEFGR
                     REEQVISAPGSISRLSVGVIVPGDLSDEKRRRITDLVRVAAGINESRGDAVVVQPLEQ
                     LGVTKAALLTGEVATESASSQSASSQTAASAAPATVIGKSAPESSTRRWVMVCLSAVG
                     LLAFGLLAGLGMARKVPGHRPPLSAAERQHLLAEMERALSGKAELGVRSTL"
     gene            complement(1632..1952)
                     /gene="fliE"
                     /locus_tag="VNZ06_08130"
     CDS             complement(1632..1952)
                     /gene="fliE"
                     /locus_tag="VNZ06_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HWW20757.1"
                     /translation="MSIESIAALAANALPAAPTQLATVAPAVSTPGAHGAFQTILSGI
                     NELNDQLQGAERSLQKLALGEGLDNLHQTMMGMERTRLSLQLLLQVRSRALEAYQELM
                     RMQI"
     gene            complement(1949..2395)
                     /gene="flgC"
                     /locus_tag="VNZ06_08135"
     CDS             complement(1949..2395)
                     /gene="flgC"
                     /locus_tag="VNZ06_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HWW20758.1"
                     /translation="MGLFNIMDISAAAMGVQRTRLEVAATNLANAQTSSASPGATYQP
                     LAAIVRSVVPSQADPSRMASGAAEDNLPVPVVAELVPMNVAPRRMYEPGHPDADAQGF
                     VNYPGIDPVSTMLDLISISRSYEANLKAFDITRSLLQRSLDIGSQR"
     gene            complement(2398..2766)
                     /locus_tag="VNZ06_08140"
     CDS             complement(2398..2766)
                     /locus_tag="VNZ06_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HWW20759.1"
                     /translation="MFSIDPVLELLSRSLDACTLRQGVYAANIANADTVGYRPLEVSF
                     EESLRSAAQLSQSAPSAQQMDVLRQIEPSIVPAAADTVQLDQQLALMDKNAIRYQMLV
                     GGFERSISLLRMAVLEGREG"
     gene            complement(2802..>3765)
                     /locus_tag="VNZ06_08145"
     CDS             complement(2802..>3765)
                     /locus_tag="VNZ06_08145"
                     /inference="COORDINATES: protein motif:HMM:NF003676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HWW20760.1"
                     /translation="TGDSSRSLVTSQTMSNLLREFGLQVPADSINSRNVAVVLVTANL
                     PPFVRAGDHLDVNVSSVGDATSLSGGTLLLTQLIGADRSSYAVAQGALSIAGFRFEQA
                     GSLAQKNHATSGLIPEGAIIQRTIVPAPIAVDGSIDLLLNEPDFTTAQRVSEAINRAM
                     LSAAASTVDAGRVRLHPGALDTAGFVSLMAAIENLSVEPSVKARVIVNERTGTIVAGG
                     EVWISQVTVAQGDIRVAINQQYLVSQPYGPVIGANSRVGTVVAPEAQISVDESQVRSV
                     TLPGATIEELVKGLHSIRASSRDVIAILQGIKRAGALHAELIIQ"
BASE COUNT          670 a         1279 c         1124 g          692 t
ORIGIN      
        1 cgccatctgc agcagattgg ctttcgcacc cgcagaaaga cgggcgagga tccattcgcg
       61 atcctcacgc gagaggcctc ccagcacttc gaccatgtcc tgcgatgaac tcacagggtg
      121 ctcctcactc ccagttctgc cttgcccgac agcgcgcgct ccatttccgc cagcaggtgc
      181 tgtcgttcgg cggcggatag tggcggccga tgaccgggca ctttgcgtgc catgcccaga
      241 cccgccagca agccaaacgc caacagtccc acagcactga ggcagaccat cacccatcta
      301 cgggtcgagg attcgggcgc gctctttccg atgacggtgg ccggcgctgc gctcgcggcg
      361 gtctggctcg atgcgctctg gctcgatgcg ctctcggtcg ccacctcccc cgtcagcagc
      421 gccgccttcg tgactccgag ctgctccagc ggctgcacga ccaccgcgtc gccacgtgat
      481 tcgttgattc ccgccgcaac acgcaccaga tcggtaatcc ggcggcgttt ctcgtcgctc
      541 agatcgcccg gaacgatcac accgacgctc aaacgtgaaa tgcttccggg cgcggaaatc
      601 acctgctctt cgcgtcggcc gaattcatat tccacctcgg tggtggagcc ggacggtttg
      661 gcgtagcccg ccgttgccga ttcgctggta accgccggtg tcgggccggc gccgctttcc
      721 acctggcgcc ggcgcagcac agccccttcc ggtccagccg gtgtgccctt catcggcacc
      781 aggtcctgca ccgtgctctt gatctgatca aaattcagtg caacatccac actcaccagc
      841 gcctgacccg atccgtaggc gctgtcgagc agcgtcccga ccttgcgggc gatatagtct
      901 tcgatgctgc gcttgatgct gaggcgcgcg tccgagccgg tagtgccgcc ctcggtaccg
      961 tcaccaccgg agagcgtgat accgcgctga tcggtgatga cgaccttcga ggcatccagg
     1021 ttggacaccg aagcggccac caggcgctgc acgcccgcag tctgctcggc actgagcgat
     1081 gcgcctgctt tcagcaccag cgacacggct gcgcgtggtt cttcgcggtc gcgtttaaac
     1141 atggtggatt ccggcagcac cagatgcacc cgcgccgact gcacgccctg cagtgcgctg
     1201 atggtgcgcg cgagttctcc ctgcagtgcg cgctggtatg cgatccgctg cgactgctcg
     1261 ctcgcgccaa ggccctcacg atcgaagatt tcaaaaccaa ccccaccgct cagcggcaga
     1321 ccgctggaga ccagcgccag gcgtgtctca tacacctgtc ctgccggtac cttgacggtc
     1381 gtgccttgat ccgcgaggcg gtagggaatc ttctggtgct tgagctgttc gacgacgttc
     1441 gccgcgtccg cttcgccgag ctgggcaaac agcacctggt agtcggtact ggatgaccag
     1501 accgccaacg cgaccgcgcc cgccacgatg agcgccgccc ccaggatcat cccggctcgc
     1561 ccgaaggtcg gcagccgtga ccagctcaca agctgttttt ctctagtgct agccattcac
     1621 ccgctctctc attagatctg catcctcatc agctcctgat aggcctccag tgcgcggctg
     1681 cgcacctgca acagcagctg cagggacaga cgcgtgcgct ccatgcccat catggtctga
     1741 tgaaggttgt ccaggccctc cccgagggcg agcttctgca gcgaccgttc tgcgccctgc
     1801 aactgatcgt tgagctcgtt gatgcccgac agaatggttt ggaacgcgcc atgcgcgccg
     1861 ggagtactga ccgcgggcgc caccgtggcc agctgcgtgg gcgccgctgg caacgcattc
     1921 gccgccaacg ctgcaatcga ttcgatgctc atcgctgact cccgatatca agactgcgtt
     1981 gcaacagact gcgtgtgatg tcgaacgcct tgaggttcgc ctcgtagctg cgtgagatcg
     2041 agatcaggtc aagcatggtg gacaccgggt cgatacccgg gtagttcaca aagccctgcg
     2101 catcggcatc cggatgtccg ggctcataca tccgccgtgg cgcgacgttc atcggcacca
     2161 gctcggcgac caccggcacc ggcagattgt cttcggcggc gccactggcc atgcgcgacg
     2221 gatccgcctg gcttggcacg acactgcgca cgattgccgc cagcggctga taggttgccc
     2281 cgggcgaggc actggaagtc tgcgcattgg cgaggttggt cgcggccact tccagccgcg
     2341 tgcgctgcac ccccatggcg gcggcactga tatccataat gttgaacagg cccatggcta
     2401 accctcccgt ccttccagca cggccatccg cagcaggctg atggatcgct cgaatccgcc
     2461 caccagcatc tggtagcgga tggcgttctt gtccatcaac gccaactgct gatccagctg
     2521 tacggtatcg gccgcggccg gcacgatgct gggttcgatc tgacgcagca cgtccatctg
     2581 ttgagcactg ggcgcactct gcgacagctg agccgcagag cgcaggctct cctcgaaact
     2641 cacttccagc ggccgatagc cgacggtatc ggcattggcg atgttggcgg catagacgcc
     2701 ctgccgcagg gtgcaggcat ccagactgcg actgagtagt tcgagaaccg gatcgatact
     2761 gaacatgaca ccttccctac cgactgtccg aacaacgccg gttactgaat aatgagttcc
     2821 gcatgcaacg cgcccgctcg cttgatgcct tgcagaatcg caatcacatc gcgcgaactg
     2881 gcacggatcg aatgtagccc cttcaccagc tcctcgatgg tggcgcccgg cagcgtgacc
     2941 gaacgcacct gtgactcatc cactgagatc tgcgcttccg gcgcgaccac cgtaccgaca
     3001 cggctgttgg caccgatgac cggtccatag ggctgcgaca cgagatattg ctgattgatg
     3061 gcaacgcgga tatcgccctg ggccacggtc acctgcgaga tccatacctc accgccggcc
     3121 acgatcgtgc cggtgcgctc attgacgatg acgcgcgcct tgactgaggg ttcgaccgag
     3181 aggttttcga tcgcagccat caggctcacg aaacctgcgg tatcgagggc gcccggatgc
     3241 aggcgcacgc gtccggcatc gaccgtggag gccgccgcgc tcagcattgc ccggttgatg
     3301 gcctccgaca cgcgctgagc ggtggtgaaa tccggctcgt tgagcagcag atcgatcgat
     3361 ccgtccaccg ctatcggcgc cggcacgatc gtgcgctgaa tgatcgcccc ctcgggtatc
     3421 aggccggagg tcgcgtgatt tttctgggcg agactgcccg cctgctcgaa gcgaaacccg
     3481 gcaatcgaca gcgcgccctg cgcgacggca taggacgaac ggtcggcgcc gatcagctgc
     3541 gtgagcaaca acgttccacc cgagagcgag gtggcatcgc cgaccgatga cacattgaca
     3601 tcaagatgat cgccggcgcg tacgaacggc ggcaaattgg ccgtcaccag gaccaccgcg
     3661 acgttgcgac tgttgatcga atcggccggc acctgcagtc cgaattcccg cagcagattc
     3721 gacatggtct ggctggtcac gaggctgcgc gaagagtctc cggtg
//
LOCUS       DATMRM010000282         3637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2009639, whole genome shotgun sequence.
ACCESSION   DATMRM010000282 DATMRM010000000
VERSION     DATMRM010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2009639"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VNZ06_08150"
     CDS             <1..869
                     /locus_tag="VNZ06_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HWW20761.1"
                     /translation="AIGVNYIDVYDRTGQYPRALPAVVGREAAGVITALGSGVRDFRI
                     GQRVAYVTGTAGAYSVARNIAANSLVRLPRAISDEQAAVLMLKGLTVEYLLRRTYRVA
                     RGDYLLVHAAAGGVGSLLTQWGRTLGAKLIGVVGNDEKAVLARKQGCHQVLISGRDKL
                     VEGVRAYTRGHMADVVYDSVGKDTFLESLDCLRPRGLMVSYGNASGPPAAIAPLELAR
                     RGSLTLTRPTLFDYIRERAELDTAARAVFSQVSSGKLKVRICQRYPLREAAQAHRDLE
                     ARRTVGATVLVP"
     gene            complement(873..1373)
                     /gene="ilvN"
                     /locus_tag="VNZ06_08155"
     CDS             complement(873..1373)
                     /gene="ilvN"
                     /locus_tag="VNZ06_08155"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HWW20762.1"
                     /translation="MRHIIGILLQNEAGALTRVAGMFSSRGYNIESLSVAPTDDPTVS
                     RLTLVTSGSEAVMLQITNQLKKLIDVVDVEDMTRGEHIERELVMLKLRVEPDRRDAVR
                     GYVVRTGGRVLDPSSHCFIVELLGSEAEISGFIRELAAHAELVEVVRSGALGISRQPS
                     LRVVTA"
     gene            1559..3415
                     /gene="ilvB"
                     /locus_tag="VNZ06_08160"
     CDS             1559..3415
                     /gene="ilvB"
                     /locus_tag="VNZ06_08160"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HWW20763.1"
                     /translation="MNDAAPKMYPATEPHRLAGNSMTGAEMIVQVLADEGVGAIFGYS
                     GGAILPTYDAIFTFNEQQKLTAGQQIPLIVPANEQGAGFMAAGYARSSGKVGVCLVTS
                     GPGATNTVTPVRDCMADSVPIVVICGQVARAAIGTDAFQEAPVASLMGAVAKHVFLVT
                     DPALLEATVRTAFEIARTGRPGPVVIDIPKDVQNSVGAFRGAGTLPIPGYRARLREVT
                     AARLSEGMANEFFGLLSQSKRPLIYAGGGVINGNAAAELSEFARAYGIPTVTTLMGIG
                     AIDTTDTLSMRMLGMHGAAFANYAADDCDFLIAVGARFDDRVAGVPRKFARSARHIAH
                     LDIDQAEINKVKDCHWHHVGSLPDALRELLSYGTRISFQPDYGLWRAELAQLKTRHAM
                     NYDRHSDLVQPHYVIEEINRHTRGEAIIATGVGQHQMWAAQMFDFCGPRLWLTSGSMG
                     TMGFGLPAAIGAQVANPKRLVIDLDGDASIRMNIGELETATTYELPVKVVVLNNFGDG
                     MVKQWQKLFFKGRLSGTDKSLHKKDFVRAATADGFKWARRLQRKQDVARVVAEFLSFP
                     GPAFLEVLIDPDAGVYPMVGPGLGYDAMITGDYIAGRAPSEAPGDVEPSGMF"
     gene            complement(3432..>3637)
                     /locus_tag="VNZ06_08165"
     CDS             complement(3432..>3637)
                     /locus_tag="VNZ06_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20764.1"
                     /translation="MGRTWGSTLVPTPQLGLLKDAGVGLRLGNARSSFGSVIHVDLAF
                     PFDHAPGVDISSVQFLVSTQQSF"
BASE COUNT          656 a         1117 c         1175 g          689 t
ORIGIN      
        1 ccgccatcgg ggtcaattac atcgacgtgt acgaccggac cgggcagtac ccgcgcgctt
       61 tgcctgcggt cgtgggccgc gaagccgccg gcgtgattac ggccttgggt tctggcgtac
      121 gcgacttcag gattggccag cgcgtggcct atgtcaccgg taccgccggg gcttacagcg
      181 tggcgcgcaa catcgcggcc aacagcctgg tcaggctgcc gcgcgcgatt tccgatgaac
      241 aggcagccgt tctgatgctg aaaggcctga cggtcgaata cctgcttcgc cgtacctacc
      301 gcgtcgcccg gggcgactat ctgctggtac acgcggccgc cggaggcgtc ggttcgctgc
      361 tcacgcagtg gggacgcacg ctgggtgcga agctcattgg tgtcgtcggt aacgacgaga
      421 aagccgtgct agcccggaaa cagggctgcc atcaggtact catcagcggc cgcgataaac
      481 tggtcgaagg cgtgcgcgcg tacacgcggg gtcatatggc cgatgtggtc tacgactcgg
      541 tgggcaagga caccttcctt gagtcgctgg attgcctgcg cccccgcggt ttgatggtgt
      601 cctacggcaa tgcctccgga ccgcctgcgg cgatcgcgcc gttggaactg gccaggcgcg
      661 gttcgctcac cctcacgcgg ccgacactgt ttgactacat cagggaacgc gctgaactgg
      721 atacggcagc cagggcggta ttcagccagg tatccagcgg caagctcaag gtcaggatct
      781 gccagcgcta cccgctacgc gaggcggcgc aggcgcaccg cgatctggag gcgcgccgca
      841 ccgtgggcgc gacggtgctg gtgccttaaa tcttaggcgg tcacgacgcg cagcgaaggc
      901 tggcggctga ttccgagggc cccgcttcga accacctcga ccagttccgc gtgcgccgcc
      961 agctcgcgga tgaagccgct gatctcggcc tcgctgccga gcagttcgac gatgaagcag
     1021 tggctggatg gatcgagcac ccggccgccg gtgcgtacca catatccccg gaccgcatca
     1081 cgccgatcgg gctcgacccg cagtttcagc atcaccaact cacgctcgat atgctcgcct
     1141 cgggtcatgt cctcgacgtc cacgacgtcg atgagtttct tgagttgatt ggtgatctgg
     1201 agcatcacgg cttcggatcc gctcgtcacc agtgtcaggc gcgataccgt tgggtcgtcg
     1261 gtcggtgcga ccgacaggga ctcgatgtta tagccacggc tggagaacat gccggcgacg
     1321 cgcgtcagcg cgcccgcttc gttctgcaga agaattccga tgatgtgtcg catgttccgg
     1381 gtcgtccgaa ggttacctgg ccgatgatac cggagtggcc ggtgcgatgg atctgcggac
     1441 agaaccgcgg gcaagccctg actgaacggt tataagccca cggcttgagt agatatatgg
     1501 ctatggctat cctgatagat gttttgccct gagctttcat acctcatcgc tagttaccat
     1561 gaacgacgct gccccgaaga tgtaccccgc caccgaaccc caccgtctcg ccggcaactc
     1621 catgaccgga gccgaaatga tcgtgcaggt gctggccgat gagggggtcg gggcgatttt
     1681 tggctatagc ggcggggcga ttttgcctac ctacgatgcg atctttacct tcaacgagca
     1741 gcagaaactg acagctgggc agcagattcc gttgatcgtg ccggccaatg aacagggggc
     1801 cggtttcatg gcggccggtt acgcacgctc cagcggcaag gtcggcgtgt gcctggtcac
     1861 ctcaggtccc ggagcgacca acaccgtgac gccggtgcgc gactgcatgg ccgactccgt
     1921 gccgatcgtg gtgatctgcg gtcaggtggc gcgcgccgcc atcggcaccg atgcttttca
     1981 ggaagccccg gtggcctcgc tgatgggcgc tgtggccaaa cacgtctttc tggtgaccga
     2041 tccggcgttg ctggaagcta cggtacgcac cgcctttgag atcgcgcgca cgggtcgtcc
     2101 cggcccggtg gtaatcgata tccccaagga cgtgcagaac agcgtcggcg cgtttcgcgg
     2161 tgcgggcacg ttgccgattc cgggttatcg cgcgcggcta cgcgaggtga cggccgccag
     2221 gctcagtgaa ggcatggcca atgaattctt tggcctgttg tcgcagtcga agcggccact
     2281 gatctatgcc ggtggcggcg tcatcaacgg caatgccgcg gccgagctgt cggaattcgc
     2341 gcgcgcttac ggtattccaa ccgtcaccac gctgatgggc atcggtgcga tcgataccac
     2401 cgatacgctt tcgatgcgca tgctcgggat gcacggcgct gcgttcgcca actatgccgc
     2461 cgacgactgc gatttcctga tcgcggtcgg cgcgcgcttc gacgaccgcg tggcgggcgt
     2521 cccacgcaag ttcgcgcgca gcgcgcggca cattgcacat ctggatatcg atcaggccga
     2581 gatcaacaaa gtcaaggatt gccattggca tcatgtcggt tcgctgcccg atgccctgcg
     2641 cgagttgctc tcgtacggca cgcgaatcag ctttcaaccg gactatggtc tctggcgcgc
     2701 cgagctcgcg cagttgaaaa cgcgtcacgc catgaactac gaccgtcaca gcgatctggt
     2761 gcagccgcat tacgtgatcg aagagatcaa ccggcacacc cggggcgaag ccatcattgc
     2821 gaccggtgtc ggacagcatc agatgtgggc ggcgcagatg ttcgacttct gcgggccgcg
     2881 tctgtggctg acctccggca gtatggggac gatgggattc ggtctcccgg ccgcgatcgg
     2941 cgcccaggtc gcaaacccca aacgcctcgt catcgatctg gatggcgacg ccagcatccg
     3001 aatgaatatt ggagagctgg agacagcgac cacctatgaa ctgccggtca aggtcgtcgt
     3061 gctcaacaat ttcggcgacg gcatggtaaa gcaatggcaa aagctgtttt tcaagggccg
     3121 tctctcgggt accgacaaat cgctgcacaa aaaggatttc gtccgagccg cgacggccga
     3181 cggtttcaag tgggcccgcc gcctgcagcg caagcaggac gtagcgcgag tggtggctga
     3241 gtttctgtct tttccgggac ctgcatttct cgaagtgttg atcgatccgg atgccggggt
     3301 gtacccgatg gtgggtcccg gcctgggcta cgatgcgatg atcaccggcg actacatagc
     3361 ggggcgcgcg ccgagcgagg cgcccgggga cgtcgagcct tccgggatgt tctagcgctc
     3421 taccctccgc ttcagaagct ctgctgggtg ctgaccagaa actgcaccga gctgatatcc
     3481 acgccgggtg cgtgatcgaa aggaaacgcc agatcgacgt ggatcacgct gccgaaggat
     3541 gagcgcgcgt tgcccaagcg aagtccaacc ccggcgtctt tcagcagtcc caattgaggg
     3601 gtcggaacga gcgtcgagcc ccaggttcgg cccatgt
//
LOCUS       DATMRM010000283         2424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1732878, whole genome shotgun sequence.
ACCESSION   DATMRM010000283 DATMRM010000000
VERSION     DATMRM010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732878"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VNZ06_08170"
     CDS             complement(<1..821)
                     /locus_tag="VNZ06_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWW20765.1"
                     /translation="MDFSHSDKVKQLQHRLTAFMEQYIYPAEAAFHEELERNRRAGNP
                     WQPTQVMEDLKARARAQNLWNLFLPHSEHGAGLTNLEYAPLCEIMGRSHLASEAFNCS
                     APDTGNMEVLARYGTPAQQERWLKPLLAGEIRSGFAMTEPDVASSDATNIQSAIVRDG
                     DQYVINGRKWWTSGAGDPRCKILIFMGKTNPQAALHSQQSMILVPIDTPGVRKIRTLN
                     VFGYDHAPHGHAQIDFVDVHVPAENMLLGEGRGFEIAQGRLGPGRIHHCMRVVGLA"
     gene            complement(832..1926)
                     /locus_tag="VNZ06_08175"
     CDS             complement(832..1926)
                     /locus_tag="VNZ06_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWW20766.1"
                     /translation="MSEPVASVEESSGALRRPLPQHAFDPAPLAAWMSQHVEKFSGPL
                     DVYQFAGGQSNPTFLIKTPQRRYVLRRKPPGKLLPSAHAVEREYRVISALRPTKVPVA
                     KTYGLCEDTSVIGSAFYIMEHVEGRIFWDAALPEVAPSDRRPIYMEMIRVQAELHSLD
                     YKALGLGDYGKPDHYIERQVARWTTQYRGAQTEKIEAMEHLIDWLPRHVPPGEQTSIV
                     HGDYRLDNTIFDPAKPRILAVLDWELSTLGHPLVDLAFHCSRFRLPASSFHGLGGLDA
                     ASLSIPSEQECVAEYCRIRAQPPIPPKDWAYYMVFCMFRLASILQGVLARALQGNASS
                     ASALEAGRRTRPLAELGWQLARQSLHAAHN"
     gene            complement(1923..>2424)
                     /locus_tag="VNZ06_08180"
     CDS             complement(1923..>2424)
                     /locus_tag="VNZ06_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWW20767.1"
                     /translation="DILVNNAGATWGSPAEKHPLPAWNKVFTLNTTSVFTLSQAVANR
                     CFIPRRSGNIIVVSSTAALRVAGLQTVAYTASKAAALHLTRALANEWGQYNVRVNAIC
                     PGFFPSKMTTELLKKIGTQVIERTPLGRLGGDTDLMGAAVYLASDASRHVTGQYIAVD
                     GGATSV"
BASE COUNT          455 a          742 c          759 g          468 t
ORIGIN      
        1 gccaggccga ccacccgcat gcaatgatga atgcgtcccg gacccaggcg tccctgggca
       61 atctcgaagc cgcggccttc gccgagcaac atgttctcgg ccggaacgtg cacatcgacg
      121 aagtcgatct gcgcatgacc gtgcggcgca tggtcgtatc cgaagacatt gagcgttcga
      181 atcttgcgca cgcccggtgt gtcgatcggt accaggatca tcgactgctg actgtgcagc
      241 gccgcctgcg gattggtctt gcccatgaaa atgagaatct tgcagcgcgg atcgccagcc
      301 cccgaggtcc accacttgcg gccgttgatg acatattgat ccccgtcgcg cacgatcgcg
      361 gactgaatgt tggtcgcatc gctcgatgcc acatccggct cggtcatcgc aaagcccgaa
      421 cgaatctcgc cggccagcag gggtttcagc caacgctcct gttgcgccgg cgtgccgtag
      481 cgcgccagca cttccatgtt gccggtatcc ggagcagaac aattgaaggc ctccgaggcc
      541 aggtgcgagc gtcccatgat ctcgcacagc ggcgcatatt cgagattggt gagtccggcg
      601 ccgtgctccg aatgcggcag gaacaggttc cacagattct gcgcgcgcgc ccgggccttc
      661 agatcctcca tgacctgcgt cggctgccag ggatttccgg cgcggcgatt gcgctccaac
      721 tcttcgtgaa aggccgcctc ggctggatag atgtactgct ccatgaaggc cgtgagcctg
      781 tgctgcagct gcttgacctt gtcgctgtga gagaaatcca tgggcttatc ctcagttgtg
      841 agcggcatgc aacgattgcc gtgcaagttg ccagccgagt tcggccagcg gccgggtgcg
      901 ccggcccgcc tccaatgcgc tcgcgctcga cgcattgccc tgcagcgcac gcgccaggac
      961 cccctgcaga attgaggcaa ggcggaacat gcagaacacc atatagtagg cccaatcctt
     1021 cggcggtatc ggtggctggg cgcgaatgcg gcagtactcg gcgacgcact cctgttcgct
     1081 tggaatcgac agactcgccg cgtccaatcc gcccagaccg tgaaacgaac tggcgggcag
     1141 acgaaagcgt gagcagtgaa aggcgagatc cacgagcgga tgcccgagcg tggacagctc
     1201 ccagtccagc acggcgagga tgcgcggttt cgcaggatcg aagatcgtgt tgtcgaggcg
     1261 atagtcaccg tgcacgatcg atgtctgttc tccgggtggc acatgcctgg gtaaccagtc
     1321 gatcagatgt tccatcgctt cgatcttctc ggtttgggcg ccgcgatatt gtgtcgtcca
     1381 gcgggcgacc tgacgctcga tatagtgatc gggtttgccg tagtcgccca agcccagtgc
     1441 cttgtagtcg agcgaatgca gttctgcctg cacgcggatc atttccatat agatcggacg
     1501 tcgatcggat ggggccacct caggcagcgc tgcatcccag aagatgcgcc cctcgacatg
     1561 ctccatgatg tagaaggcgc tgccgatgac ggaggtgtcc tcgcacaatc catacgtttt
     1621 cgcgaccggc actttcgtcg ggcgcagcgc ggaaatgact cgatactcgc gctccacggc
     1681 atgggccgaa ggcaacagtt ttcccggtgg cttgcgacgc agcacgtagc gcctctgcgg
     1741 ggtcttgatc aggaaagtcg gattggattg cccgccggcg aactgataca catcgagcgg
     1801 tccggaaaat ttctcgacgt gctggctcat ccaggccgcg agtggagccg gatcaaacgc
     1861 gtgttgcggc agcggccggc gcagagcccc cgaactctct tccaccgaag cgaccggctc
     1921 gctcataccg aagtcgctcc cccatccacg gcaatatatt gcccggtgac atgtcgagat
     1981 gcatccgagg ccagatagac cgccgcgccc atcagatcgg tgtcgccgcc gaggcggccg
     2041 aggggtgtgc gctcaatgac ctgggtcccg atcttcttga gcaactcggt ggtcattttc
     2101 gagggaaaga accccggaca gatggcgttc acgcgcacgt tgtactgacc ccattcgttt
     2161 gccagcgctc gtgtcagatg gagtgcggcg gccttggatg cagtgtaggc gactgtctgc
     2221 aaaccagcga cccgcagtgc cgcggtcgat gacacgacga tgatgtttcc tgaacggcgc
     2281 gggatgaagc agcgattggc gaccgcctgg ctgagcgtga agacgctggt ggtgttgagg
     2341 gtgaacactt tattccaggc gggcagcgga tgcttctccg ccggtgagcc ccaggtcgcg
     2401 cccgcgttgt tgaccaggat gtcg
//
LOCUS       DATMRM010000284        19041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4516435, whole genome shotgun sequence.
ACCESSION   DATMRM010000284 DATMRM010000000
VERSION     DATMRM010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19041
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4516435"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..593
                     /locus_tag="VNZ06_08185"
     CDS             156..593
                     /locus_tag="VNZ06_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWW20768.1"
                     /translation="MSMNLGSSEEDRGIMSEINTTPLVDIMLVLLIIFLITIPVITKT
                     VKVQVPKAINIPTQTKPENITISITRDGEVFWGQSHVPTMAQLVDQIERQAVKVPQPE
                     IHIRADAQTDYKNVGRVIAAINKGGLVKVGFITEPDRAMVPMR"
     gene            651..1043
                     /locus_tag="VNZ06_08190"
     CDS             651..1043
                     /locus_tag="VNZ06_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWW20769.1"
                     /translation="MCDINTTPLIDVMLVLIVTLIVTLPIMTHAVKLDMPPPNPNQPP
                     PPVQPDIINLDIYYDGTVAWNGQTIPTLDELEQQFQVAAAKDPQPELHLAPDSHVKYD
                     VVAKVLASAQRNHMAKIGFVNTSEFLEE"
     gene            1168..2385
                     /locus_tag="VNZ06_08195"
     CDS             1168..2385
                     /locus_tag="VNZ06_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWW20770.1"
                     /translation="MRNRLKVVSVLASMCVIGALSGVALAADQPPQPSKGTVKILKAA
                     QDAIKEKKLDEALTHLKEAQTVPDRTAYDNFVINQDLLYIYVQKQDYAAAAPILESTA
                     QSPYAKPEDQKAWLKALFAIDYQQKDYQKTVDVGEQLIKRGISDAQTLSTIAEAQKQL
                     GKFKDAAATVDQIVSHQEKPEEELLRFQWTMYDKVKDEADAAKVLEKLVSYYPKPDYW
                     AGALGSLLKMDIKDSHLQLNVYRLENDVGVLKRPADFADMAELGLDAGFPGETVAVLE
                     EAFQKGVFTEQRDKDRYQHLLDGAKQRAASDQQSLGRTEPVDGNACVQLGAAYMSYGQ
                     YDKAVANISKGLQKGGLKSVDEANLLLGIAFLRQKNNSEATKAFDKVASSSNPGYARL
                     GKLWALRAGQRSA"
     gene            2426..2908
                     /locus_tag="VNZ06_08200"
     CDS             2426..2908
                     /locus_tag="VNZ06_08200"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWW20771.1"
                     /translation="MTIKVGDRMPEGKFKTMTENGPRDLSTSDLFNGKKVVLFSVPGA
                     FTPTCDAKHLPGFLAQSEALKSKGIDTIACMAVNDVFVMSAWGKSSNVGGKVLMLADG
                     NGEYSRALGLELDAKGAGMGMRGQRFAILVDNGVAKQVNIEAPGQFKVSAADHVLSQL
                     "
     gene            2987..4270
                     /locus_tag="VNZ06_08205"
     CDS             2987..4270
                     /locus_tag="VNZ06_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWW20772.1"
                     /translation="MSSEYLVKPSDSLKNVRYEIRGRLARRAHEMERQGYEIISLNIG
                     NPRLFGFRTPETMRLAMIENLSDAEGYCHQKGIFPAREAVVMQQQARGITGVSAEEVF
                     IGNGVSELIDLVLRALLDDGDEVLVPSPDYPLWTAAVNLNRGRAVHYACRPQNHFIPD
                     PAEIESQITQHTRAIVVINPNNPTGATYPRPVLEAIARIAECHQLVVFSDEIYDQMVY
                     DGAESIPMATLVRDTLCATLSGLSKIYRACGYRVGWVVFSGRLRGTAEYLSALELLSS
                     LRLCSNVPAQWAVQTALGGYQSIRELVSPGGRLYESRRALLEAVRRSQFLQLTETNGA
                     MYGFVGVKTEAIPEFDDQQFALDLLEQKHVLIAPGVSFNVPYRNYFRVTTLPDAAMLK
                     SVFGRIEELLAAQATTPTKSADERRTVVKAQGRFK"
     gene            4358..7006
                     /locus_tag="VNZ06_08210"
     CDS             4358..7006
                     /locus_tag="VNZ06_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWW20773.1"
                     /translation="MPSRQRASAVRLAYSAMMLAQGRELWDSPDALPWSAWLERELDD
                     ARARGESPPPRLTATEEWLLWQEAVHEACGGRGVLMPDGLIDPVRRAVGLLDDYGLTL
                     SDVPTPEVATLRQSMLHFRRRCEDLHVLSRSSWRELMLWLRPSARLLIADSTGIGRER
                     LRWLMQHEARVATPGGETGRCQVFDFETASHEADAAASWCAAQLASDAAARLLVVVPR
                     LEEARHLWERAFFQRLNHSLIVAGHERSTESGFAIEGGRALTSYPLVSTALHLIALAS
                     GRAGFTQLSEVLRSVYLSVVPRAARLMFDRWLRERNIASAQLALLRSVLPLLQQAVGE
                     NAAAAAKTLLDALEAAGRSRLAMPSDWAQTWAALLGRCGWPGALPLGSDEQQIRMRFD
                     ELLGDFAAAQLLGGRLTPEEASSRLQAMALRVSFEPASDDVPVTLTARLEDPIVRYDG
                     IWVAGLSSDVWPPAARPDPLLPLLLQYASAMPTATADGQSGLARQRQERWRDSTRQCV
                     FSWSRTHDDLPHDRSPLLPLEAAGTGTGLHTAQGSRALQDATLEAWYAAQAPVLEAWT
                     ERTTPASSVDGVLRGGTRLLELQSLCPFRAYAELRLQAVPLPQPAPGINPQVRGMILH
                     QALELFWKKIVDQTTLRMRHSAGGAELQDLIHGSVAHALRETLAREPGTVSEQMKQRE
                     HARTVGLINEMLKWELERPPFVTSILESPRRCQFEGAAIDFRLDRIDVLEGGHELIID
                     YKTGKPKPFDPLAERLRQPQLPAYAVASGPMTAGVAALYLGREGVTVRGSADAPSRLN
                     GRLAPKDGEPSWHARREQWRQRLQRLVDEYLRGDASVDPLQDACKLCHLHPLCRIDPA
                     VLAALDTDPDNPEGEESEEESEEQSE"
     gene            7003..10320
                     /locus_tag="VNZ06_08215"
     CDS             7003..10320
                     /locus_tag="VNZ06_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWW20774.1"
                     /translation="MSAADAREQDARARREALDTSRSLLLQAPAGSGKTTVLTARFLA
                     LLAVVDQPEEILAVTFTRKAAAEMRHRVIAALESAGRQASLRGIPDELLKAAAQRNLA
                     QGWDLLAQPSRLRIETMDALNYWLANQLPIAARASPGLQITPDPQSLYRRAARRCLES
                     AQGDPVMAAAADLVFHRLDNNWAGIENRLAEMLAQRAHWLPRLTGSTAPELVARIEES
                     LRSTLRSELAYAIAPIPAGLLRESEIILAHSLRELGRGVPATGVRLSAEPISVREWRA
                     LARLGLVADRKGWRQALTKSQGFPSKDKPWNERGKLWLAAMSSTPGAFDALCSVACLP
                     DSELSHADRQALEALSQLLIRAAQELQIEFATTGKVDFTCISGAARAALMEQSDPTDL
                     ALRIGTAIRHILVDEFQDTSQDQLGLLRAMTASWQQGDGRTLFVVGDPMQSIYAFREA
                     EVGLFLQSRDHGIGDIRFEGLQLRQNFRSRDAVIEWVNQRFSKLFPSTDDVRLAAIRY
                     LPSTPGPVQIPGDGPAVHLHAFEERDFESEAARVVKIVQDARKRSPQASIAVLVAARL
                     HATILVAKLSAAGEVVRGVHLEPLNERPVVRDLSSLTRSLLHGGDRGAWLALLHAPWC
                     GLTLADLESLEFENHGDLFALLADASHRLDEQQGRRLQRLCEALRPAIEGTERGLPLW
                     QRVERCWLRLAGPAIYRSEIERLDARRFIDAVAEHEEPEALVGDAISQLLKDLYSSAP
                     PRTGAIEVMTMHAAKGLEWDIVILPGLGRRTAPTLDRLLHWMELPRPREGTDLLLAPI
                     RATDKEIEESLAGYIKRLKRRRQEFERERLLYVAATRARRTVHLLGGLVLPKRGGLPE
                     PYRYSLLWTLWRAIGEEFLARYHEVTAAPRKRPAMPTTLPGLLRLPAEWRLPEMPALP
                     RVMRVNAGSPVPETTPEYSWVGRTARAVGTIVHAELRRAAESGFANDLRHYDAWLLEL
                     GVALKDLSSASARIREALRRTLADPQGRWLLARDHREAQSEWHLTGMHEGRVINVIFD
                     RMLVDDKGQRWVIDYKTSTHEGGAVQEFIDREADRYRPQMRRYAILAKRIDAAPVRVA
                     LYFPLLGVFRELTFVSE"
     gene            complement(10312..11040)
                     /locus_tag="VNZ06_08220"
     CDS             complement(10312..11040)
                     /locus_tag="VNZ06_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HWW20775.1"
                     /translation="MSLRTGILLAALIFILTLVVRLPARLLVSYLPAEVSCEDPGGTV
                     WEGSCGRVRMNGVTVAGLSWKLHPLALLRATLSADVSSSDPNNGGRASIEATRSGDLS
                     VSELHAVLPFPPGSNVMPRGTSATLTLALAWARIHETHLVSIIGTIGLQQLHIADPPA
                     DLGSFELQFPATESSTMTGQLRDLEGPLAVNGQVVLQPSGTYEINGTTAPRAALSEDL
                     SKALQFLGPPDANGQRTFSLAGSL"
     gene            complement(11040..11525)
                     /gene="gspM"
                     /locus_tag="VNZ06_08225"
     CDS             complement(11040..11525)
                     /gene="gspM"
                     /locus_tag="VNZ06_08225"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HWW20776.1"
                     /translation="MIEQLQRYLLGLTPRERYIVIGGSVVALVLVVLAIVLPLQSRVS
                     AAQERVQQKRNDLVWLRQVGPQLASLQSTRPPGVLNESLAALVDRTARESGFSKNLAG
                     TQASGDGGLNVRIDKMAFDAMVTWLSQLHERYGVSVDTAAVDSAGEPGTVSATLVLRA
                     G"
     gene            complement(11522..12781)
                     /gene="gspL"
                     /locus_tag="VNZ06_08230"
     CDS             complement(11522..12781)
                     /gene="gspL"
                     /locus_tag="VNZ06_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HWW20777.1"
                     /translation="MAEWLVLQLSRKPEDQCGWMFADERGQPLSAPRVGTLAAAGQEA
                     AGRRLAVLVPSGDVLMTDVELPVKSGVRAQQVAPFALEEQLAADIETLHFAVGPRDDV
                     SNKTSVAVVTRDLMRQWTEALEAANLQPEIVCAEAALLPDNPGHTVVLLDRETLNARR
                     AGGTPLALSAADVAAALEASLGPDLATENVIFYSTPQDWHRHASQIEAIRPRCASFKA
                     QLLNAGILPLLASQLMSGQFVNLLSGDYAPKRSLGSDWRRWRLAASLAAALLVVHVSG
                     LSYEWVQQRHSEQQLDDAIGQVARNAIPGDPGTGSVRARVQARLASQSGGSAAGLLPA
                     LSAVAQAVSSVNGATVESLNYRKEGMELRLKAHDATSLDRIDQTLRNNGYQADITSGA
                     QNGADYEGHIEVHTAAAADPKPARRSP"
     gene            complement(12784..13731)
                     /gene="gspK"
                     /locus_tag="VNZ06_08235"
     CDS             complement(12784..13731)
                     /gene="gspK"
                     /locus_tag="VNZ06_08235"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HWW20778.1"
                     /translation="MRSLIRQGGVALITAIILVAIAAVLATAIGYASAMSARRASTQF
                     GSDQSFLAAQGAEAMAAYVLRLSTQNNAPFSLDQPWAQAYGPFELTDGVTMELLQLED
                     EQSKFNINNLAPNGSTNQDAKKLFEQILQLVGLETKWAGIIADWIDSDNEPNSPDGAE
                     DSAYTSQTPPYRTANIPITSISELLALPGFGRARYDRIAPYITALPPGSDINICTTSG
                     LILDAIAGKVEYSSDPDALANERKLNGCFPKLTVFQSTLQSDAQAQKFSSLFLGSSTS
                     YFRLRSFITIGSARFTLYSLLYRDNSGQMRPILRTFGTE"
     gene            complement(13728..14408)
                     /gene="gspJ"
                     /locus_tag="VNZ06_08240"
     CDS             complement(13728..14408)
                     /gene="gspJ"
                     /locus_tag="VNZ06_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HWW20779.1"
                     /translation="MSYRSGRGFTLIELIVALFIAAIMFAFGYRALSQAINSRQEVDQ
                     QAVRLQALQTTMRILEQDFELAEPRPVRNTIGDGYQPAFSAPATTGFTGSVSTQLSGT
                     SLKGQSPPIVTFTRVGWSNPVGIQRSEMQRVMYTIEDGSLTRSYYPVLDATEGVVPVK
                     RALIDHIKSFTLRFMDAGHNWQTSWPPLTLGGVPQLTQLRYRPIAVEIRMEIEGWGVL
                     IRHIEVAG"
     gene            complement(14405..15214)
                     /gene="gspI"
                     /locus_tag="VNZ06_08245"
     CDS             complement(14405..15214)
                     /gene="gspI"
                     /locus_tag="VNZ06_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HWW20780.1"
                     /translation="MRRVSGFTLIEVLVALVIVAFGMSAVLAALSSSADNISALREKS
                     LAEWIAMNRIADERLNLNPPKPGFTEGDLKGFGNEDWHWREDVVAVDAIPGMLEIAVR
                     VAHLKPGQTSFSSGSSTSSKPTRTASGFSGGAPGSFFSSSGYSSGSYSSGGFSSGLGG
                     LSSSLKNLGASALPVSADTQWVATVIGFRGDAVSPASGEQPDWTCSGGGALTASGLCN
                     ISNTQGASSSGATINQNTGQSPGSQPLLNPTGSPTYPPSPAPIGSSAGGTP"
     gene            complement(15211..15834)
                     /gene="gspH"
                     /locus_tag="VNZ06_08250"
     CDS             complement(15211..15834)
                     /gene="gspH"
                     /locus_tag="VNZ06_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HWW20781.1"
                     /translation="MPYRWPAYRPQRGFTLLEVLVVMLIIALLTSAAIISLGSTGKDS
                     ELEKERDRLAALMSYVRERGAMLTIEYGIRCAPHGYRFVYFDNRTNQWQPEIVDDTLR
                     LRHLPAGLNLSLVIEGHQIVLDDKNLTIPKALTTPAGGKLNTLGSDMPSTFETQNADN
                     APQVLLLSNGDVNSFSLKLERQGTRRSATLQSKADESIATGPILEPK"
     gene            complement(15855..16304)
                     /gene="gspG"
                     /locus_tag="VNZ06_08255"
     CDS             complement(15855..16304)
                     /gene="gspG"
                     /locus_tag="VNZ06_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HWW20782.1"
                     /translation="MNALQTRTAGVQRGFTLIEIMVVVIIIGLLAAVVVPQFLGNVDK
                     ARVAKAKQDIQALETALTMYKLDNFTFPTNEQGLKALVEKPNDPNVKNWRLGGYLKHL
                     PNDPWQNPYVYQYPGTHGEFDIYSMGADGQPGGDGLNADIGNWNLDN"
     gene            16439..16972
                     /gene="rrtA"
                     /locus_tag="VNZ06_08260"
     CDS             16439..16972
                     /gene="rrtA"
                     /locus_tag="VNZ06_08260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HWW20783.1"
                     /translation="MTVIALILVLAALGDRGREALSYDRDALAHFQLWRALTAHWVHL
                     SWRHALLNCAGLVVLWALFAREFSPRRWLWILTLAAVAIDLGLWFLHPTVVWYVGASG
                     VLHGAWSAGACAAYRRGDGMGAVIMLLLVVKLAYEQQAGVSIFEGDLPLVTEAHLLGA
                     FGGLLGALMPRATTKPL"
     gene            17007..17660
                     /locus_tag="VNZ06_08265"
     CDS             17007..17660
                     /locus_tag="VNZ06_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HWW20784.1"
                     /translation="MSIALVFPGQASQLVGMLGAMTACFSRVRECFDQASGALGFDLW
                     KLVCEGPAERLNSTEFTQPAVLVAGVATWRVWRDQGGAAPALVSGHSLGGFLRVLARW
                     ASFSGWRTNKGIKIRRPKFRYLSAMDCTEHVEPADIRATLAQPLANPVRWTTTVRAVL
                     AVASIVIECGPGKVLTGLNRRIERTATCYALEDPDSLTAALAATTSNGNSRASHASL"
     gene            17647..18387
                     /gene="fabG"
                     /locus_tag="VNZ06_08270"
     CDS             17647..18387
                     /gene="fabG"
                     /locus_tag="VNZ06_08270"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HWW20785.1"
                     /translation="MLPSDLALVTGASRGIGRAIALSLGRAGATVIGTATTADGAQKL
                     AAELNSAGVKAHGVVWQADQAASTEALVAQLDAGPGLPNILVNNAGIARDALILRMKS
                     EDWEHTLAVNLTSVFRLTKACLRRMLKDRHGRIISIGSVVGSIGNPGQVHYAAAKAGL
                     VGFTKALAKEVATRNITVNIVAPGFIETDMTRALPQPAREAYLGQIPIGKFGTSEDVA
                     NAVLFLASPEAGYMTGQTLHVNGGMYMG"
     gene            18555..18797
                     /gene="acpP"
                     /locus_tag="VNZ06_08275"
     CDS             18555..18797
                     /gene="acpP"
                     /locus_tag="VNZ06_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWW20786.1"
                     /translation="MSTVEQQVKAIVAEQLGVKAEQVTNAASFVDDLGADSLDTVELV
                     MALEEEFEIEIPDEDAEKITTVQQAVDYISERRTKA"
     gene            18807..>19041
                     /locus_tag="VNZ06_08280"
     CDS             18807..>19041
                     /locus_tag="VNZ06_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HWW20787.1"
                     /translation="MSKRRVVITGLGLVSPVGGSVTDSWAAVLRGESGVGPITRFDVS
                     AYPTRIGGAVKGFSIEQYLSAKEARRMDEFMHYG"
BASE COUNT         3685 a         5539 c         6137 g         3680 t
ORIGIN      
        1 cggtcatggg ctacaactgg ctgctgcggc ggaataagat caatcaggat gatgtgcgga
       61 acttcgcgag cgatttgcat gcctacttgg taagtggcgc gcgtgtcacc ggtgccggtg
      121 gctgatcact caacagtcaa cccggagctg ccatcatgtc tatgaatctg ggatcgtcgg
      181 aagaagatcg cggaatcatg tcggagatca acacgacgcc gttggtcgat atcatgttgg
      241 tgttgttgat catctttctg attaccattc ctgtgatcac caaaacggtt aaagtgcagg
      301 tgccgaaggc catcaatatc cccacgcaga ccaagcccga gaacatcacg atatcgatca
      361 ccagggacgg cgaagtgttc tgggggcagt ctcacgtgcc gaccatggct cagctggtcg
      421 atcagatcga acgtcaagcg gtgaaggttc ctcagccaga aatacacatc cgcgccgacg
      481 cccagaccga ctacaagaac gtcggcaggg tgatcgccgc tatcaacaag ggcggcttgg
      541 tgaaagtggg attcatcacc gagcccgacc gcgccatggt cccgatgcgc tagaggatca
      601 ggagacaaac gcttatggca atgagtgcag gaaccaccga aggcgcacct atgtgcgaca
      661 ttaacaccac gccactgatc gacgtcatgt tggtgttgat cgtgacgctg attgtgaccc
      721 ttccgatcat gacgcacgcg gtaaagctgg atatgccgcc gccgaatccg aatcagccgc
      781 cgccgccggt acagccggac atcatcaatc ttgatatcta ctatgatggg acggttgcct
      841 ggaacggtca gaccatcccg acgcttgatg agctggagca acagttccag gttgccgctg
      901 ccaaggaccc gcagccggaa ttgcacttgg ctccggacag tcacgtgaag tacgacgtcg
      961 tcgccaaggt actggcgtcc gcccagcgca accatatggc gaagatcggt ttcgtgaata
     1021 ccagcgagtt tcttgaggaa tagtgtaccc cgtgcgcggt gctacatcgg actagaccca
     1081 cgctcttata ggaggcgcag gagtggcgaa gctggttgtc cccggtaatc gtgccactgc
     1141 atcgcgcccg aactatcgag gaagcccatg aggaatcgtt tgaaagttgt cagtgtcctg
     1201 gcatccatgt gcgtcatcgg cgctctgtcc ggtgtcgcgc tcgctgcgga ccaaccgccg
     1261 caacccagca aggggacggt caagatactg aaggccgcgc aggatgctat caaggagaag
     1321 aagctcgacg aagcgttgac tcatctgaag gaagctcaga cggtgccgga tcggaccgcg
     1381 tacgataact tcgtcatcaa tcaggatctg ctctacattt acgtgcagaa gcaggattac
     1441 gccgctgctg cgccgatcct ggagagcacc gcgcaatcgc cgtacgcgaa acctgaggac
     1501 cagaaggcct ggctgaaggc actgttcgcg attgactatc agcagaagga ctatcagaag
     1561 acagtcgacg tcggtgagca gctcatcaag cgcggcatca gcgatgcgca aacactgtcc
     1621 accattgctg aagcgcaaaa gcagctgggt aagttcaaag acgcggcggc caccgtggat
     1681 cagatcgttt ctcatcagga aaaacccgaa gaggagctac tgcggttcca gtggacgatg
     1741 tacgacaagg tgaaggatga agcggatgcc gcgaaggtgc tggaaaagct ggtgtcctac
     1801 tatccgaaac ctgactattg ggctggagca ctgggctcgc tgctgaagat ggacatcaag
     1861 gactctcacc tgcagctcaa cgtgtatcgt ttggagaacg atgtcggtgt cctgaaacgc
     1921 cctgcggact tcgccgacat ggctgaactc gggctcgacg ctggattccc aggggaaact
     1981 gtggcggtgc tcgaagaggc tttccagaag ggcgtgttca ccgagcaacg cgataaggat
     2041 cgttatcagc acctgctgga cggcgccaag cagcgcgcgg ccagcgatca gcaatcgttg
     2101 ggtaggactg agccggtcga tggcaacgcc tgcgtgcaac taggtgcggc atacatgagc
     2161 tacggccagt acgacaaggc tgtggccaac atcagcaaag gtctgcagaa aggtgggctg
     2221 aagtccgtcg atgaggccaa tctgctgctg gggattgcct tcttgcgcca gaagaacaac
     2281 tctgaagcga cgaaagcgtt cgacaaggtc gccagttcga gcaatcccgg ctacgcccgg
     2341 ctgggcaagc tctgggctct gcgtgccggg cagcgtagcg catgaccgtc gggcgctaac
     2401 aagttaccta aggagtcgca ctgccatgac gatcaaagtc ggtgaccgca tgcccgaggg
     2461 caagttcaag acgatgaccg agaacggacc tcgggacttg agcacgtcag atctgttcaa
     2521 cggcaagaag gtggtgctgt tttcagttcc cggtgcgttt accccgacgt gtgatgcgaa
     2581 gcatctgccc gggtttctgg cgcagtccga ggcactcaag tccaagggta tcgatacgat
     2641 tgcctgcatg gcggtgaacg acgtgttcgt catgagtgcc tggggcaagt cctcgaacgt
     2701 cggcggcaag gtgttaatgc tggcagacgg taacggggag tattcccgag ccctgggtct
     2761 ggaactggat gccaagggag cagggatggg aatgcgcgga cagcgcttcg cgatcctggt
     2821 ggacaacggg gttgccaagc aggtgaacat cgaggcgcct ggccaattca aggtctccgc
     2881 agccgatcac gtgctgtcgc agctctagcg gctgctgccc agtcaattta gctgactgcg
     2941 accggctcgt cgtgcacggt gcgaccgagc gcggtactct tggcccatga gcagcgaata
     3001 cttggtcaag cccagcgatt ccctaaagaa cgtccgctac gagatccgcg gcagactcgc
     3061 ccgccgggct cacgagatgg agcgtcaggg ctacgagatc atttcgctga acattggcaa
     3121 tccgcgcctg ttcggctttc gcacccccga gacgatgcgg ctggcgatga tcgagaatct
     3181 gtcggacgcg gaaggatact gtcatcaaaa gggcatcttt ccggcgcgcg aagcggtcgt
     3241 catgcagcag caggctcgtg gcattaccgg cgtcagtgcg gaagaggtct tcatcggtaa
     3301 cggcgtcagc gaactcatcg atctggtgtt gcgcgcactg ctcgacgatg gcgatgaggt
     3361 gctggtgcca agcccggact atccgttatg gacagccgca gttaatctca atcggggacg
     3421 cgcggtacac tatgcctgcc gaccccagaa tcacttcatt ccggatcctg ctgaaattga
     3481 gtcgcagatt acgcagcaca cacgtgcgat tgtcgtcatc aatcccaaca accccaccgg
     3541 tgcgacctac ccgcgcccgg tactggaagc gattgcacgc atcgccgagt gtcatcagct
     3601 ggtggtattc agcgatgaga tctacgacca aatggtctat gacggtgccg agtcgattcc
     3661 gatggcaacg ctggtgcgtg acacgctgtg tgcaacgctc tcggggctct cgaagatcta
     3721 tcgtgcttgc ggctaccgcg tagggtgggt ggtgttttcg ggacgattgc gcggaacggc
     3781 cgaatactta tcggcgttgg agttgctgtc gtcgctgcgc ctgtgcagca atgtaccagc
     3841 tcaatgggcg gtgcagacgg cgctcggtgg ctatcagagc attcgcgaac tggtcagccc
     3901 gggaggaaga ctctatgaat cacgtcgagc gttgctcgag gccgtcaggc gaagtcagtt
     3961 tctgcaactc accgagacca acggcgcgat gtacggcttt gtcggcgtca aaactgaagc
     4021 gattcccgaa ttcgatgatc agcagttcgc gctcgacctg ctcgaacaaa agcacgtact
     4081 cattgcaccc ggtgtgagct tcaatgttcc ttatcgcaat tactttcgcg tgacgacgct
     4141 gcccgatgcg gcgatgctaa agagcgtatt cggtcggatc gaggagctgc tggctgccca
     4201 ggcgacgacg ccaacgaagt cagccgacga acgacgcact gtcgtcaaag cccaaggtcg
     4261 atttaagtaa tacaacagat aatatctata tctacctgat ttgcttgaac ttagcaccga
     4321 gataatagaa catctaaaga gtggcggcac cttactggtg ccaagccgtc agcgcgccag
     4381 tgcggtgcgt cttgcctata gcgccatgat gttggcccag ggccgcgagc tttgggacag
     4441 tcccgacgca ttgccttgga gcgcctggct cgagcgcgag ctcgatgacg cgcgcgccag
     4501 aggagaatcc ccgccgccgc gcctgacggc gacggaggag tggctgttgt ggcaggaggc
     4561 cgtacacgag gcgtgcgggg gccgcggcgt gctgatgccc gatggcctga tcgatcctgt
     4621 ccggcgcgcg gtcggtctgt tggacgatta cggtctgacg ctgagcgatg ttcccacccc
     4681 tgaagtggcc acgctcaggc agtcgatgct ccattttcgt cgccgctgtg aggatctaca
     4741 cgtactgagc cgctcttcat ggcgcgagct gatgttatgg cttcggccct ctgcgcgact
     4801 attgatcgcg gattcgactg gaatcggtcg cgaacgcctg cgctggctga tgcaacacga
     4861 agcacgcgtc gcgacgccag gcggcgagac tggacgctgt caggtattcg atttcgaaac
     4921 ggcttcgcac gaggcggacg ccgctgcgag ttggtgcgca gcgcagctgg cctcagatgc
     4981 cgcggcgcgt ttgctggtcg tggtaccgcg actcgaggag gcgcgacact tgtgggaacg
     5041 cgccttcttt cagagactga atcacagcct gatagtcgcc ggccacgagc gtagcaccga
     5101 gtccggcttt gcgattgaag gaggacgagc gctgaccagc tatccgctcg tgtccacggc
     5161 gcttcatctg atcgccttgg caagcggtcg cgcaggtttc acgcaactca gcgaagtgct
     5221 gcgctctgtg tacttgtcag tggttccgcg tgcagcgcgc ttgatgttcg accgctggct
     5281 gcgcgagcga aacatcgcca gtgcgcaatt ggcattgctg aggtcggtat tgccgttgct
     5341 gcagcaagct gtgggagaga atgccgcggc agcagccaag accttgctcg atgcgctgga
     5401 ggcggccgga agatccaggc tcgctatgcc gagtgactgg gcccaaacgt gggctgcgct
     5461 gctcggacgc tgcggctggc cgggagcgct gccgcttggc agcgacgagc agcagatacg
     5521 catgcgcttt gatgaactgc tcggggactt tgcggctgca cagctactgg gtggccgact
     5581 gacgccggag gaggcgagct cgcgcctgca ggccatggcg ctgcgcgtct catttgagcc
     5641 ggcttcggat gacgtcccgg tgacactgac agcacggctc gaggatccca tcgtgcgata
     5701 cgatgggatc tgggttgcgg gcctgtcatc ggacgtgtgg ccgcctgcag ctcgaccgga
     5761 tcccttactg ccgctcctgc tgcagtacgc ctcggcgatg cctacggcca cggccgatgg
     5821 acagtccggg ctcgctcggc aacggcagga gcgatggcga gactcgacgc ggcagtgcgt
     5881 gttcagttgg tcaagaaccc acgacgacct accgcacgat cgcagcccgc tgttgccact
     5941 cgaagccgct ggcaccggga cagggttgca caccgcacag gggtctcggg cgctgcagga
     6001 cgccacgctg gaagcatggt atgccgcgca agcgccggta ttggaggcgt ggactgagcg
     6061 gacaaccccg gcgtcctcgg tggacggcgt gctgcgggga ggaacacgac ttctggaact
     6121 gcaatcgctg tgtccgtttc gcgcatatgc cgagctgcgc ctgcaggccg tgcccctgcc
     6181 tcaacccgcg ccgggaatca atccgcaagt gaggggcatg attctgcatc aggcccttga
     6241 gctgttctgg aagaagatag tcgatcagac gacattgcga atgagacatt cagccggtgg
     6301 cgctgaactg caggatctca ttcatggcag cgttgcgcac gctttgcgag agacgctggc
     6361 gcgcgagccc ggaaccgtca gtgagcaaat gaagcagcgc gagcacgcgc gtaccgtggg
     6421 gttgatcaac gagatgctca agtgggagct tgagcgcccg cccttcgtga cgagcatcct
     6481 cgaatcgccg cggcgctgcc agttcgaggg tgccgcaatc gatttccgcc tcgaccgtat
     6541 cgatgtgctc gagggcggcc acgagctcat cattgactac aagaccggca agcctaaacc
     6601 tttcgatccg ctcgcagagc gccttcgcca gccgcaactg ccagcatatg cggttgcctc
     6661 cggcccgatg acggcgggcg tggctgccct gtatctgggt cgagaagggg tcacggtgcg
     6721 tgggtccgct gatgcaccct cgcgcctaaa cggccgtctg gcgccgaagg atggcgaacc
     6781 gtcgtggcat gcgcgacggg agcaatggag acagcggctg cagcggctcg tagacgaata
     6841 tctgcggggt gatgcgagcg tggacccgct ccaggacgcc tgcaagctgt gtcatctgca
     6901 tccgctgtgc cggatcgatc cggcggttct cgccgcgctc gataccgatc ccgataaccc
     6961 ggaaggtgag gaatctgagg aagagtccga ggagcagagc gaatgagcgc cgcggacgct
     7021 cgcgagcagg acgcgcgtgc tcggcgcgag gcactcgata cgtcacgttc cttgctgctc
     7081 caggctccgg ccggatccgg caagaccacg gtgctcactg cgcgttttct ggcgttgctg
     7141 gcggtggtgg atcagcccga ggagattctg gcagtcacat tcactcgcaa ggcagctgcc
     7201 gagatgcggc atcgagtgat cgcggcgctg gaatccgccg gcaggcaggc atcgcttcgc
     7261 ggcattccgg acgagctgtt gaaggctgca gcgcagcgca atctcgcgca gggctgggat
     7321 ctccttgctc aaccgagccg gcttcggatt gaaacgatgg atgcgctcaa ctactggctc
     7381 gctaaccagt tgccaatcgc tgcacgcgcc agtccgggtc tgcagatcac ccccgatcca
     7441 cagtcgctgt atcggcgagc agcccgtcga tgtctcgaat cggcgcaagg tgatccggtg
     7501 atggccgccg ccgctgacct cgtgttccat cggctcgaca acaactgggc ggggatcgag
     7561 aaccgattgg ccgagatgct ggcgcagcgc gcacattggt tgccgcgact gacgggatcg
     7621 accgcgccgg agttggtcgc gcgcatcgag gagagcctca gatcgactct acgcagtgag
     7681 ctggcttacg cgatcgcccc catcccggcc gggttgctgc gcgagagtga aatcattctt
     7741 gcacattcgc tgcgcgaact aggccgtggg gtgccggcga caggtgtgcg tttgtccgct
     7801 gagccgatca gcgtcagaga gtggcgagct ttggctcgtc tcggattggt cgctgacagg
     7861 aaggggtggc gacaggcgct cacaaagagc cagggatttc ccagtaagga caaaccctgg
     7921 aacgaacgcg gcaaactttg gctggctgca atgagtagta cgccgggagc attcgatgcc
     7981 ctgtgctcgg tggcgtgtct gcccgattct gaattgagcc acgccgatcg acaggcgctg
     8041 gaagcgctgt cgcaactcct catacgggcc gcccaggagt tgcagatcga gtttgcaacg
     8101 actggcaaag tggatttcac ctgtatcagt ggggcggcgc gtgcggcgct gatggaacag
     8161 agcgatccca ccgatctggc gctgcggatc ggcaccgcca ttcgccacat cctggtcgat
     8221 gagtttcagg atacatcgca ggatcagctc ggcctgctgc gggcgatgac ggcgagttgg
     8281 cagcagggcg acggccgaac attgtttgtg gttggcgacc cgatgcagtc catctacgcg
     8341 ttcagggagg ccgaagttgg actgttcctg cagtcccgcg accacgggat cggcgacatc
     8401 cgctttgagg ggttgcagct gcgacagaat ttccgctcgc gcgacgcggt cattgaatgg
     8461 gtcaaccagc gcttcagcaa actgtttccc tccacggacg atgttcgcct ggcagcgatc
     8521 cgctatctgc cgtcgacacc gggaccggtg cagatacccg gtgacggacc ggctgtgcac
     8581 ttgcatgcct tcgaggagcg cgatttcgag tcggaggctg cccgagttgt gaagatcgtg
     8641 caggacgcgc gcaaacgttc accgcaggca tcgatcgcgg tgctggttgc cgctcgtttg
     8701 catgcaacca ttctggtggc aaaactgtct gccgccggag aagtggtgcg cggcgttcac
     8761 ctggaacctc tgaatgaacg tccggttgtg cgtgatctga gctccttgac gcgcagcctg
     8821 ctgcatggcg gggatcgcgg tgcctggctg gcgttgctgc acgcgccctg gtgcggactc
     8881 acgctggccg atctggagtc gctcgagttc gaaaatcatg gtgatttgtt cgcgctcttg
     8941 gccgacgcca gtcatcgatt ggatgaacag cagggtcggc ggttgcagcg attgtgtgaa
     9001 gcccttcgac ccgccataga agggactgag cggggcttgc cgctatggca gcgcgtcgag
     9061 cgctgctggc tgcgcctcgc cgggccggct atctatcgca gcgagatcga gcggcttgac
     9121 gcacgtcgtt tcatcgatgc tgtggcagaa catgaggagc cggaagcgct tgtcggtgac
     9181 gcaatctcgc aactgctgaa ggacctctac tcgagtgcgc caccccggac cggggcgata
     9241 gaggtcatga cgatgcatgc cgccaaaggg ttggaatggg acatcgtcat cctgccgggt
     9301 ctgggccgca ggacggcacc gacactcgat aggttgctgc attggatgga acttccccgc
     9361 ccgcgggaag gcaccgatct gttgctcgct ccgattcgcg cgaccgacaa ggagatcgag
     9421 gagtcactcg cgggctatat caagcgactc aagcgccgcc gacaggagtt cgaacgcgag
     9481 aggctgctct acgttgccgc gactcgtgcc cgcaggaccg tgcatctgct gggagggttg
     9541 gttctgccga agagaggggg actgcccgag ccctatcgct attcgctgct atggacgttg
     9601 tggcgagcga ttggcgagga gttcctcgcg cgctatcacg aggtgacggc cgcgccgcgg
     9661 aaacggccgg cgatgccgac aacgctgccc gggctgctgc gtttgccggc ggagtggcgc
     9721 cttccggaga tgccagctct gccgcgcgtg atgagggtga atgcagggtc gccggtgccg
     9781 gagacaacac ctgagtacag ttgggtagga cgtaccgccc gcgctgtggg tacgatcgtg
     9841 cacgcggaac tgcgaagggc cgccgaaagc gggttcgcca acgatctgcg ccactatgat
     9901 gcgtggctgc tggagctggg tgttgcgctc aaggatctgt catcggcgag tgctcgcatt
     9961 cgcgaggcgc tgcgacgcac gctggcagat ccgcaagggc gttggttatt ggccagagat
    10021 caccgtgaag cgcaaagcga atggcacctg accgggatgc atgagggacg ggtcatcaac
    10081 gtgatcttcg atcgcatgtt ggtggatgac aagggtcagc gctgggtcat cgactataag
    10141 accagtaccc acgagggcgg tgcggtgcag gagttcatcg atcgcgaggc cgatcgctac
    10201 cggccgcaga tgcggcgcta tgcgatcttg gcgaagcgca ttgacgcagc accggtccgg
    10261 gtcgcgctct attttccgct gctcggtgtc ttccgcgaac tgacgttcgt ctcagagtga
    10321 accggccagc gagaacgttc gctgaccgtt ggcatcaggc ggtccgagaa actgcagcgc
    10381 tttgctgaga tcttcgctga gggcggcgcg tggcgccgtg gtcccgttga tttcgtatgt
    10441 gccggaaggt tgcagcacga cctggccgtt taccgcgagc ggcccctcca gatcacgcag
    10501 ctgaccggtc atcgtcgatg attcagtcgc cggaaactgc aattcaaagc tgcccagatc
    10561 cgcgggcgga tcggcgatat gtagttgctg caagccgatc gtaccgatga tcgagaccag
    10621 atgagtctca tggatccttg cccaagccaa ggcaagggtc agcgtggcac tggtcccacg
    10681 aggcatgacg ttcgatccgg gaggaaacgg cagaacggcg tgcagctcgc tgactgacag
    10741 gtcgccgctg cgggtggctt cgatactggc acgaccgccg ttgttgggat cagagctact
    10801 cacatcggca ctgagggtcg cccgcagcag tgccagcggg tgcagcttcc aggacagacc
    10861 ggcgacggtg acgccgttca ttcgtacgcg tccgcacgag ccttcccaca cggtaccacc
    10921 ggggtcttca caactgacct cggccggtag ataggagacc aacagcctcg ccggtagccg
    10981 gactaccaag gtgaggatga aaatcagcgc ggcgagcagg atgcccgttc gaagcgacat
    11041 cagccggcgc gcaacacgag cgttgcggag accgtcccgg gctcacccgc agagtcaacg
    11101 gcagccgtat cgacgctcac cccgtagcgc tcatgcagtt gcgacagcca cgtgaccatc
    11161 gcatcgaagg ccatcttgtc gattcgcacg ttgagcccgc cgtcgccgct ggcctgcgta
    11221 ccagccagat ttttgctgaa accggattca cgggccgtgc gatcgaccaa cgcggcgagg
    11281 gattcattca atacgccagg aggacgagtg gactgcaacg acgcgagttg ggggcccacc
    11341 tgacgcagcc agacaaggtc attgcgcttt tgctggacgc gctcctgcgc tgcactgaca
    11401 cggctctgca atggcagcac gatcgccaga acgacgagga ccaacgccac gaccgagcca
    11461 ccgatcacga tatagcgctc ccggggagtg agcccgagca agtagcgctg cagttgctcg
    11521 atcatggtga acgtctcgcg ggctttggat cagcggcagc ggcggtgtgc acctcgatat
    11581 gcccttcgta atcagcgccg ttttgcgcgc cggaggtaat atccgcctgg tagccgttgt
    11641 tgcgcagagt ctgatcgatt cggtccaggc tggtggcgtc gtgtgccttg aggcgcagct
    11701 ccatcccctc tttgcgatag ttcaacgact ccacggtcgc accatttacg ctgctgaccg
    11761 cctgggcgac cgcgctgagc gcaggcagta gtcctgcggc gctgccgccg gactgggacg
    11821 caagcctggc ctgcacccgc gcgcgcacag agccggtgcc gggatctccg ggtatcgcgt
    11881 ttctcgccac ttgaccgatc gcatcatcca gctgctgttc actgtgccgc tgttgcaccc
    11941 attcatagga caaacccgag acatgcacta ccagcagcgc ggccgccaaa gaggcagcca
    12001 gacgccagcg tcgccagtca ctgccgagcg aacgcttcgg ggcgtaatcc ccgctgagca
    12061 ggttgacgaa ctgtccggac atgagctgcg aggccagtag cggcaatatg ccggcattca
    12121 gtaactgcgc tttgaagctc gcgcaacgcg gtcggatcgc ctcgatctga ctcgcatgac
    12181 gatgccagtc ctggggtgtc gagtagaaga tcacattctc agtcgcgagg tcggggccaa
    12241 gcgatgcctc gagtgccgcg gccacgtctg ctgcggacaa tgccaaaggg gtgccgccgg
    12301 cgcggcgggc gttcaacgtt tcgcggtcca gcaaaaccac cgtgtgtccg ggattgtcgg
    12361 gcagaagcgc cgcttcggcg catacgatct ccggctgcag gttcgctgct tccagcgcct
    12421 cggtccactg cctcatgaga tcgcgggtca cgacagcgac ggacgtcttg ttgctgacgt
    12481 catcgcgcgg accgacggcg aagtgcaatg tctcgatgtc agccgccagt tgttcttcga
    12541 gcgcgaaggg ggcaacctgc tgcgcgcgca ccccgctctt gaccggcagt tcgacatcag
    12601 tcatcagcac atcgccgctg ggtaccagta cggcaaggcg gcggccggcc gcttcctggc
    12661 cagccgcggc gagggtacct actcgcggtg cgctgagggg ctggccacgc tcatcggcga
    12721 acatccagcc gcactggtct tcaggcttgc gcgacagctg cagaacgagc cattccgcca
    12781 tggttattcc gtgccaaaag ttcggagtat gggtcgcatc tgcccgctat tgtcgcgata
    12841 caacagactg tacaaggtga accgggcact gccaatagta ataaatgaac gcagtcgaaa
    12901 gtaactcgta ctgctgccca ggaacagtga gctgaatttc tgtgcctggg cgtccgattg
    12961 gagggtggac tggaagacgg ttagcttcgg aaagcagccg ttcaacttgc gctcgttcgc
    13021 gagcgcgtcg gggtcagagg agtattcaac cttgccggcg attgcatcca gaatcagtcc
    13081 gctggtggta catatgttga tgtcgcttcc cggtggaagc gcggtgatgt agggggcgat
    13141 gcggtcgtag cgggcgcgac cgaacccagg cagtgccaat agttccgaaa tactcgtgat
    13201 tgggatgttg gccgttcggt agggcggcgt ctgggatgtg taggcactgt cctccgcgcc
    13261 gtcgggcgaa ttcggttcgt tgtcggagtc gatccagtcg gcaatgatgc ccgcccactt
    13321 ggtttcgagt cccacgagct gcaggatctg ttcgaacaac ttcttcgcgt cctggttagt
    13381 actaccattc ggagcaagat tgttgatgtt gaacttgctt tgttcgtctt cgagctgcag
    13441 gagttccatc gtgacgccgt cggtcaattc aaacggccca taggcttgcg cccagggctg
    13501 atcgagtgag aacggtgcat tgttctgtgt agacagccgc agtacatagg ccgccatggc
    13561 ctcagcgccc tgcgcggcga gaaagctctg gtccgagccg aactgcgtac tggcgcgccg
    13621 ggccgacatg gcgctcgcat agccgatcgc tgtcgccagc accgctgcga tggccaccag
    13681 aatgatcgcc gtgatcaggg caacaccgcc ttgacgtatg agcgacctca tccggccacc
    13741 tcgatgtgcc ggatcagcac gccccatcct tcgatttcca tccgtatctc gactgcgatc
    13801 ggccggtagc gcagctgcgt aagctgcggg acgccaccga gggttagcgg cggccagctg
    13861 gtttgccagt tgtggccggc gtccatgaag cgcagggtga aactcttgat atgatcgatc
    13921 agcgcacgct tgaccggtac gaccccttcc gttgcatcga gcaccggata gtaggaacgg
    13981 gtcaacgagc cgtcctcgat cgtgtacatg acgcgctgca tctcactgcg ttgaatgccc
    14041 accggattgc tccagccgac acgggtgaac gtcacgatgg gtggactttg acctttgaga
    14101 ctggtcccgg agagctgcgt gcttacggag ccggtgaagc cggtagtggc cggcgcactg
    14161 aaggctggct ggtagccgtc gccgatcgtg ttgcgcaccg ggcgaggttc agccagctcg
    14221 aaatcctgtt ccaggatccg catcgtggtc tgcaatgcct gcaggcgcac ggcttgttga
    14281 tcgacttcct ggcggctgtt aatcgcttga gacagcgcgc gataaccgaa cgcgaacatg
    14341 atcgccgcga tgaacagcgc gacgatcagc tcgatcaggg taaagccgcg cccactgcgg
    14401 tagctcacgg cgtacctccg gccgaactgc caatgggcgc tggactgggt gggtaggtgg
    14461 ggctgccggt cggattgagc agcggctgcg agccggggct ttgccccgtg ttctgattga
    14521 tcgtcgcacc cgaactgctg gcgccctgag tgttactgat gttgcacaga cccgaggcgg
    14581 ttagggcacc accaccgcta caggtccagt caggctgttc accgctggca ggcgagaccg
    14641 cgtcgcctcg gaagccgatg acggtggcga cccactgcgt atctgcgctg accggcaacg
    14701 ccgaggcccc tagattcttc aacgatgagg acagtccacc cagtccggag ctgaagcctc
    14761 cggaactgta actgccggag ctatagccgc tgctggagaa gaaactgccc ggtgcgccac
    14821 cggaaaatcc cgacgcggta cgggttggct tactggaggt ggagctgccg gaagagaagg
    14881 aggtctgtcc cggcttcaga tgcgcgacgc gcaccgcaat ttccagcatt ccgggtattg
    14941 cgtcgaccgc cacgacatcc tcgcgccagt gccagtcttc attgccgaaa cccttcaggt
    15001 cgccttccgt gaagcccggc tttggcggat tgagattcag acgttcatcg gcgatccgat
    15061 tcattgcaat ccattcggcc agactcttct cacgcagtgc ggaaatgttg tctgcggacg
    15121 agctcaatgc cgcaagtacc gcgctcatgc cgaacgcgac gatgacgagc gcaaccagca
    15181 cttcgatcaa ggtgaatccg ctgacgcgtc tcacttcggc tccaggattg gcccggtggc
    15241 aatgctctcg tccgctttgc tctggagcgt cgcgctgcgg cgagtgcctt gacgctcgag
    15301 cttcagcgaa aacgaattga catctccgtt agagagtagg aggacttgcg gagcgttgtc
    15361 ggcgttctgt gtctcaaaag tgctgggcat atccgagccg agggtattga gtttgccgcc
    15421 cgccggggtc gtcagtgctt tagggatcgt cagattcttg tcgtccagca cgatctgatg
    15481 accctcgatg accaggctta ggttcagtcc agctggcagg tgacgcagtc gcagcgtgtc
    15541 atcgacaatc tccggctgcc attggttggt gcggttgtcg aagtacacga accgatagcc
    15601 gtgcggtgcg cagcggatgc cgtattcgat cgtcagcatc gcgccgcgtt cccgcacata
    15661 ggacatcagt gccgccagac gatcacgttc cttctcgagt tcactgtcct tgccggtgct
    15721 gcccaggctg atgatcgcgg cactcgtcag cagtgcgatg ataagcatta ccaccagcac
    15781 ttcgagcagc gtgaaaccgc gctgcgggcg gtatgccggc cagcgatatg gcaccctgag
    15841 cgtgggccta atcctcagtt atcgaggttc cagttcccga tatcggcgtt caggccgtcg
    15901 ccgcccggct gtccgtcggc gcccatcgaa tagatatcga actcgccgtg agtgccgggg
    15961 tactggtaca cgtaggggtt ctgccacggg tcattgggca gatgcttcag atagccaccc
    16021 agacgccagt tcttgacgtt cggatcgttg ggcttctcga ccagcgcttt cagaccctgc
    16081 tcattggtcg ggaaggtgaa attgtccagc ttgtacatcg tcagcgcggt ttcgagcgcc
    16141 tggatgtcct gcttggcctt ggcgacgcgc gccttgtcga cattgccaag gaactgcggc
    16201 acgacaacgg ccgccaaaag accgatgatg atcacgacca ccataatttc gatgagcgtg
    16261 aagccgcgct ggacgccggc tgtacgggtc tgaagagcgt tcatgtagtg tgccgagggc
    16321 atggtgggaa ttccagtcaa tccgccaatc ataacaaatg accgcttata cgactgacag
    16381 ccgcgcacgg tcttggttcc ggtcggcgaa ttgcgacgct cgcctcgggt tagcgctttt
    16441 gacagttatc gcgctgattc tggtgctggc ggccttggga gatcgcgggc gggaggcgct
    16501 ttcctatgat cgcgatgcgc tggcgcattt ccagttgtgg cgcgcgctca ccgcacattg
    16561 ggtgcacctg agttggcgcc acgcactact gaactgtgca ggcctggtgg tgctgtgggc
    16621 cttgtttgcc cgcgaattca gcccgcggag atggttgtgg attctcacat tggcggcggt
    16681 ggcaatcgac ttgggtctgt ggttcctgca cccgacggtg gtttggtatg taggagcctc
    16741 gggagtgttg cacggcgcgt ggtcggcggg tgcctgcgcg gcgtatcgcc gtggagatgg
    16801 catgggcgcg gtgatcatgc tgttactcgt ggtgaagctg gcgtacgagc agcaggccgg
    16861 cgtgagtatc ttcgaaggcg acctgccgct ggtgaccgag gcgcacctgt tgggtgcgtt
    16921 cggcgggctg ctcggtgcac tgatgccgcg cgcgacgacg aagccgttat aatccgcgcc
    16981 gttttgcatg cggcagcgtc aagataatgt cgatcgcact ggtctttccg ggacaagcct
    17041 cgcagttggt ggggatgctc ggggcaatga cggcgtgttt cagccgggtc cgagaatgct
    17101 tcgatcaggc ctccggggca ttaggctttg atctgtggaa gctcgtctgc gagggtcccg
    17161 cagagcgcct caacagcacc gaattcactc agcccgccgt gctggtcgcg ggtgtggcca
    17221 cgtggcgagt ctggcgggat cagggcggcg cggcgccggc cctggtatcg ggccatagcc
    17281 tcggtgggtt cctgagggta ctggcgcgat gggcgtcatt ctcgggctgg aggacgaaca
    17341 agggcatcaa aatccgccgg ccgaaatttc ggtatctgag cgccatggat tgcacagagc
    17401 acgtcgaacc cgcggacatc cgggcaacgc tggcgcaacc gctggcaaac ccggttcgct
    17461 ggacgacgac ggtccgcgcg gtactggctg tggcgtcgat agtgatcgag tgcgggccgg
    17521 ggaaggtgct gacggggttg aaccggcgca tcgagcgcac cgcaacctgc tatgcgttgg
    17581 aagatcctga cagtctgaca gcagccctcg cggcgacaac cagcaacggc aactcgagag
    17641 cctcacatgc ttccctctga cctggccctg gtaaccggtg cttcgcgcgg aatcggtcgc
    17701 gcgatcgcac tgtcgctcgg acgcgccggc gcaacagtca tcgggacggc aacgactgct
    17761 gatggcgcgc agaagctggc cgcagaactg aacagtgccg gcgtcaaggc tcacggcgtg
    17821 gtctggcagg ccgatcaggc cgcgtctacg gaggcactag tcgcgcagct cgacgctggt
    17881 ccgggcttgc ccaacattct ggtcaacaat gcaggcatcg cgcgtgatgc gttgattttg
    17941 cggatgaaga gcgaggattg ggaacacacc ctggcggtca atctgacctc ggtcttccgt
    18001 ctgacgaaag cgtgtctgcg ccgtatgctg aaggatcggc acggacgcat catcagcatc
    18061 ggctcagtgg ttggatccat cggcaatcca ggccaggtcc actacgcggc ggccaaggca
    18121 gggctggtgg gattcaccaa agcgcttgcc aaagaggtgg ctacacgcaa catcacggtc
    18181 aatatcgtcg cacccggctt catcgaaacg gacatgacgc gagcgctgcc gcagcccgcg
    18241 cgcgaggctt accttggaca gattccaatc ggcaagtttg gcacttccga ggatgtggcc
    18301 aatgcggtgt tgtttctggc atcgccagag gccggctaca tgacaggcca gacgctgcat
    18361 gtgaacggcg gcatgtacat gggctaaaag tcaaataatc tggataatca agtactcgcg
    18421 ctcctcttaa ctggccagcg cgcaggggtt cccctacaat acgcgccccg atgtgcggca
    18481 cccgacttta acggcagcgt gcatcggtga gttcagggga tataagagtc atcacgaaag
    18541 agggctaatg aaagatgagc accgttgaac aacaggtgaa ggcgattgtt gctgagcagc
    18601 tcggagtcaa ggcagagcaa gtcaccaacg ccgcctcgtt cgtcgacgac ctgggcgccg
    18661 attcactcga cacggtcgag ctggtcatgg cgctcgaaga ggaattcgaa atagagattc
    18721 cggacgaaga cgccgaaaag atcaccactg tgcaacaggc cgtcgactac atcagtgagc
    18781 gacgcaccaa agcctgatcg cgcttcgtga gtaaacgccg tgtagtcatc accgggctcg
    18841 gtctagtctc ccccgtcggg ggcagtgtca ccgactcctg ggctgccgtt ctgcgcggcg
    18901 aaagtggcgt cggtccgatc acgcggttcg acgtctcggc gtacccgact cgcatcggcg
    18961 gggcagtcaa gggcttttcg atcgaacagt acctgagcgc gaaggaagcg cgccgcatgg
    19021 atgagttcat gcactacggc a
//
LOCUS       DATMRM010000285         3411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2011852, whole genome shotgun sequence.
ACCESSION   DATMRM010000285 DATMRM010000000
VERSION     DATMRM010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011852"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..556
                     /locus_tag="VNZ06_08285"
     CDS             65..556
                     /locus_tag="VNZ06_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWW20788.1"
                     /translation="MTQTPDVPKLEPIGTSGDGRRHYYDEQARQALVEACLQPGASLA
                     GLALKAGVNANLLRKWVGKHRAKRDAAAAAPVTGGVPAAFIPVVEVEEVATRPAPPAE
                     SEPLPAPRQPARASQRPPLSSRVVAQLPNGVSVELECTGQDAALVTAMIETLGRCHVP
                     ARR"
     gene            537..890
                     /gene="tnpB"
                     /locus_tag="VNZ06_08290"
     CDS             537..890
                     /gene="tnpB"
                     /locus_tag="VNZ06_08290"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HWW20789.1"
                     /translation="MFRLDADLRVYLHREAVDFRAGINGLVTRVEQSMQLDPFARAVF
                     AFRNRRANRIKLLFYDRSGFWLMMKRLEADHFVWPRRQQTVVELTSEQLHWLLDGIDI
                     DAVRRHPERQYRYTG"
     gene            1088..2599
                     /locus_tag="VNZ06_08295"
                     /pseudo
     CDS             1088..2599
                     /locus_tag="VNZ06_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558094.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2964..3224)
                     /locus_tag="VNZ06_08300"
     CDS             complement(2964..3224)
                     /locus_tag="VNZ06_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20790.1"
                     /translation="MAQTTAQRQAAYRARRASAGKDGNGERRLDMWISTEADLALARL
                     AGRYAATKRQMLERLISRADDAILRRLDPDSKPWDLYFNGPR"
BASE COUNT          653 a         1120 c         1065 g          573 t
ORIGIN      
        1 ccacatagct tcctgatgcc agcaggcgca gcatgtgtcc acctcaacaa caggtggaca
       61 cctcatgaca cagactcccg atgtcccgaa gctcgagccc atcggcacaa gcggtgatgg
      121 ccggcggcac tactacgacg agcaggccag gcaggcgctg gtcgaggcct gccttcagcc
      181 aggcgcatcc ctggcgggcc tggcactcaa ggccggcgtg aacgcgaacc tgttgcgcaa
      241 gtgggtcggc aagcaccggg cgaagcgcga cgccgccgcg gcggcgccgg taactggagg
      301 tgtgccggcg gcgttcattc cagtcgtcga ggtggaggag gttgcgacga gacccgcgcc
      361 gcccgcggag tcagagccgc tgcccgcgcc ccgccagccg gcacgtgcgt cgcaacgtcc
      421 gccgttgtcc tcgcgcgtgg tagcgcagtt gccaaacggc gtaagcgttg agcttgagtg
      481 cacgggacag gatgccgcac tggtgacggc catgatcgaa acgctgggac gctgccatgt
      541 tccggctcga cgctgacctg cgggtctacc tgcaccgcga ggcggtcgat ttccgggccg
      601 ggattaacgg tctggtcacg cgggtcgaac agtcgatgca gcttgatccg tttgcccgtg
      661 ccgtctttgc gttccgcaac cggcgggcca accgcatcaa gctgctcttc tatgaccgct
      721 cgggtttctg gctgatgatg aagcgcctgg aggcagacca tttcgtatgg ccacgccggc
      781 agcaaacggt ggtcgagctg acgagcgagc agctgcactg gttgcttgac ggcatcgata
      841 tcgatgcggt gcgccgtcac ccggaacggc agtaccggta cacgggctga cggtttgccc
      901 cgcgctgcgg ccgttgcctg taaacaacga cggggcaatg gtgacctggc cgtcatcccg
      961 gccaggcgct atcctgggtg gtatggatgc caacaagccc aacccctccc tcgtggacga
     1021 tctcaaacac ctcccgcacg atgcgctgat cgcgcgcatt ctcgagcttg agaaccgcaa
     1081 ccaccagctc gcgcagcgga tcgcccagct cgaggagcag ttccggctcg cgcagagcaa
     1141 gcgctttgcc ccgagcagcg agaagctcaa ggaccgcgca ttcgacgaag ccgagcagat
     1201 ggccgcggca gcgccatccg gggacgacga gccggacgac gcgttcgcgc tgccggacac
     1261 ggggctgccg cagcccgatg agcccgcccg cggcaagcgt ggtcgcaagc cgctgccggc
     1321 agaactgccg cgtcagcgca tcgagtacga cctgcccgaa gaccagaagg tctgtccgtg
     1381 ctgccgcagc gcgatgcatc gcatgggcga agaagtcagc gaacaactgc acatcgaagt
     1441 gaaggcctcg gtgctgcagc atgttcgctt caaatacgca tgtcgccact gcgagcgcaa
     1501 tgccgagcgc acgccgatcg tgaccgcgcc gatgccggcg cagccgctgc ccggcagcaa
     1561 cgccagcgcg gcgatcatcg cgaccgtgac ggctggcaaa tatgtcgatg gcacaccgct
     1621 gtaccggatg gaagacgcgc tggcgcgcgc gaacatcccc gtaagccgcg gcacgctggc
     1681 caactggatc atccggcccg ccgagctcca ctacagccga ctgtacgacg cgctgcgccg
     1741 cacgctgctg tcgcagccgc tgattcatgg cgacgagacg acggtacagg tgctcaagga
     1801 gccgggcaag accgcgcaaa gtcagtcgta catgtggacc tatcgcagcg ccgaggactg
     1861 cgagcagccg gtggtgctgt tcgactacca gcccgggcgc ggccaggagt atccgcaggc
     1921 gtttctcgcc ggctatgagg gtatgttgat gaccgatggt tacgccgcgt ggcgcacgct
     1981 cgaaggtccg acccacttcg gatgtcttgc ccatgcccgc agagcgttcg tcgatgcgct
     2041 caaggggcag aagaagccag gtggtcgcgc agcgcaggcg cttgagtact tcaaggccct
     2101 ctaccaggtc gagacgctcg ccagagcgga tctgcccgaa ggcgagacgc gcacggccta
     2161 cacgtaccgg ctgcgccagc agcacagcgt gccgctgctc acggccttca aggcgtggct
     2221 ggacgaacac gagtcgctgg tgctgcccga gagtctgctc ggcaaggcga tcgcctacac
     2281 gcgcaatcaa tggaagtacc tcagccgcta cgtcagcgat ggccatgcgc ccctcgataa
     2341 caacgtcatg gaacgggata tcagaccctt ctgtaccggt cgcaagtcat ggttgttcca
     2401 tgacacggtc gccggtgcga aagcgagcgc aatggtctac agcctgatgc tcacgtgtcg
     2461 cgcttgcaac gtcgagccgt atgcatacct gctccacgtg ctcacggagt tgcctcagcg
     2521 ggcaccggat tccgatatca gtgacctgct gccgttcaac ttcgccaaac agcaaactgc
     2581 gacgccagct tccgcctgaa cgacaaactg ctcgcgcgcc cctgttcgtt gttacccggg
     2641 aacggtgcac agtgaacact attggccgac gttgaagtag acgccccatt gcgtcgagcc
     2701 gggatcgagc ctgcgcgcac gatcccgtcg tcggcccgca caatcagacg ctcctgatgg
     2761 gtattccggt gaacgtaagc gcggattccg gaaagctcgg gaatcggtca cgtccgtccg
     2821 gaatagccgg tcacgatgaa acggaatgct cgatcacgct cgtccggaat cgtcggtcat
     2881 gttggtctgg aatacccacg catccaggct gggcactcgc cttgccgata aaacgtcatc
     2941 cgttacccgg taacgacggc gcgctaccgg ggaccgttga aatagagatc ccacggcttc
     3001 gaatccggat cgagcctgcg caagatcgcg tcgtctgctc gcgagatcaa ccgctccaac
     3061 atctgacgct tcgtggccgc gtagcgtccg gccagtcgtg caagagcaag atcggcttct
     3121 gtactgatcc acatgtccag ccgacgctct ccgttgccgt ccttgcccgc cgaggctcgc
     3181 cttgcccgat aggcggcctg ccgctgtgct gtcgtctgcg ccatcctgcg actcctcatc
     3241 attgttaccc ggtaacgatc tggttcctca acgttaccgg gtaacgacac gattcctcaa
     3301 ggtgtggagg attgagcgat tacgctcaat tccgccaccg tcagcaatcc ggcagattgc
     3361 cgacgtagaa tccagaccgg cccaatgacg gcaaaggccg aacttcagcc g
//
LOCUS       DATMRM010000286         3747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2329466, whole genome shotgun sequence.
ACCESSION   DATMRM010000286 DATMRM010000000
VERSION     DATMRM010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2329466"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VNZ06_08305"
     CDS             <1..518
                     /locus_tag="VNZ06_08305"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWW20791.1"
                     /translation="VVGESLQLVNGGSWRNAIKQRSVSDEQAVVGVSFRDAQAYCRAQ
                     RQRLPTEDEWEYVARGPEHHIFPWGDNSGPATRNSSSAPRVSDGPAEGIGLRYHGLSG
                     NVWQWVDTTAADGTKYLKGGSWLVDNPANRRAAVQRMEQPSRADADSGFRCARSVALW
                     PDADLWLARLK"
     gene            497..1246
                     /locus_tag="VNZ06_08310"
     CDS             497..1246
                     /locus_tag="VNZ06_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWW20792.1"
                     /translation="MAGTFEVAAAHSDRSVRIPAPLLDAACQPYRSAGRFAYYFARGK
                     LKSDPVYAAILSMGLLQGRSRILDLGCGQGLLAAWLRAAERCAEGGSWPYSWPAAPKS
                     VSTLGIELMLADVERARRALGADAQFLHGDIRTVTYGQADAVVILDVLHYLGAPSQRE
                     ILRRVREALPVDGVLLLRVGDADGGFRFRYGEWTDRIIMWLRGHGRVATHCRSTTQWR
                     ALLEECGFESAALPMSQGTPFANVLLVARAR"
     gene            complement(1243..1932)
                     /locus_tag="VNZ06_08315"
     CDS             complement(1243..1932)
                     /locus_tag="VNZ06_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20793.1"
                     /translation="MSDLSVQPRASVRFIWGQIGVILSLALAPAALQAGIVRDVLASV
                     GLAKPSPPPNPAAGAPVFPRQGFACCNLHRDGSSINDGNYAKYPLIPAGTAIEVLKYG
                     RNEASIKINGKALRLVHDYGRDQETLDQWVNKIVVNVDPTPRIASYPPDVQAAIHDGK
                     ILVGMTREQAIVAIGYPLTSENASLDEPIWRMWRSSHGEYDLHFADNGRLGSVTGDDE
                     VIPLMLWQPGH"
     gene            2262..>3747
                     /locus_tag="VNZ06_08320"
     CDS             2262..>3747
                     /locus_tag="VNZ06_08320"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWW20794.1"
                     /translation="MKTNLKVTYAVVLALASARGAMVYAADVDADADTGNMLGEVVVT
                     AERRTQSIQDVPITIQAITGEALKTLSVDTLDDVIKYTPNVTLGTNGPGQADIYMRGL
                     SAGSVGGQSTATFASFPNVAVYLDDQSLQFPARNADVYMVDMERIEVLEGPQGTLFGG
                     GAEAGAIRYITNKPKLDATSGNAEATYGWTTHGDNNTALNATLNVPLIDNALGARLVI
                     YDDRRGGYIDNVYSQFTRSNNDSGNYYAGIKPGANGLCPNGQPSTSGFCVPLNNTVGS
                     NTGLARSASNPVEYNGLRASVLWKISDDWDALITQSFQNLEADGLSTQYPIGSNGQAL
                     GPWQVTEFVPTYDKDRYENTALTVNGKIGDLKAVYTGGYLDRHIDQVNDYSNYTRSAY
                     GFYYTCTGGPGGGGFGTAGTGPGVPPGGSAPVCYSPITSWRDQVTNTHLSQEFRVSTP
                     DDWRIRGILGAYYENFKIRDDMNFYYKTIPGCNALNLSVALGGGP"
BASE COUNT          701 a         1170 c         1213 g          663 t
ORIGIN      
        1 ccgtggtcgg tgagagcctg cagctggtga acggaggcag ttggcgcaat gccatcaaac
       61 agcgctccgt tagcgacgag caggccgtgg tgggtgtgag ttttcgggat gcgcaggcct
      121 actgccgcgc gcagagacag cgcctgccca ccgaagatga atgggagtat gtcgcgcgcg
      181 gtccggagca ccatatcttt ccgtggggcg acaatagcgg cccggccacg cgcaacagca
      241 gctcagcgcc gcgagtgagt gatggtccgg ccgagggcat cgggctgcgc taccacggcc
      301 tgtcgggcaa cgtctggcag tgggtggaca ccaccgctgc ggacggcacc aaatacctga
      361 agggcgggtc ctggctggtc gataacccgg ccaaccgccg cgcggcggtg cagcggatgg
      421 aacagcccag ccgcgccgac gcggacagtg gatttcggtg cgcgcgcagc gttgccctct
      481 ggccggatgc ggatctatgg ctggcacgtt tgaagtagcc gctgcacaca gcgaccggtc
      541 agttcgcatt cccgctcccc tgctggatgc ggcctgccag ccctatcgca gcgccggacg
      601 cttcgcgtac tacttcgccc gcggcaaatt gaagagcgac ccggtctatg ccgcgatcct
      661 ctcaatggga ctgctgcagg ggcgttcgcg gatactggat ctgggctgtg ggcagggact
      721 gctggcggca tggctgcgtg ccgcggagcg ctgcgctgaa ggtggcagct ggccgtacag
      781 ctggccggcc gcaccgaagt cggtatcgac gctgggaatc gagctgatgc tggctgacgt
      841 ggagcgagcc cggcgcgcgc tcggggcgga tgcacagttc ctgcacgggg atatccgcac
      901 ggtcacgtac ggccaggccg atgcggtcgt gattctggat gtgctgcatt acctgggcgc
      961 gccctctcag cgcgagatcc tgcgacgagt cagggaagcg ttgccggtgg atggcgtgtt
     1021 gctattgcgg gtcggcgacg cggatggcgg ctttcgtttt cgctatggag aatggaccga
     1081 ccggatcatc atgtggctgc gcggtcatgg ccgggtagcg acccactgtc gcagcacgac
     1141 ccagtggcgc gccctgttgg aagagtgcgg tttcgagagc gccgccctgc ccatgagtca
     1201 ggggacgcct ttcgccaacg tactgctggt ggcgcgcgcc cgttagtgtc ccggctgcca
     1261 gagcatcaga ggaatgacct catcgtcgcc ggtgaccgag ccgagccgac cgttatctgc
     1321 gaagtgcagg tcgtattccc cgtgactcga gcgccacatg cgccagatgg gctcgtcgag
     1381 tgaggcattt tcgctggtga gcgggtagcc gatcgctacg atcgcctgct cccgggtcat
     1441 accgaccaga atcttgccgt catggatcgc cgcctgaacg tcaggcggat agcttgcgat
     1501 gcgaggcgtc gggtccacgt tgaccacgat cttgttgacc cactgatcga gtgtctcctg
     1561 atcgcggccg tagtcgtgca ccaggcgcag cgctttgcca ttgatcttga tactggcttc
     1621 gttgcgcccg tacttgagga cctcgatcgc ggtcccggcc gggatcagcg gatacttggc
     1681 gtagttgccg tcgttgatcg agctgccatc gcggtgcagg ttgcagcagg cgaacccttg
     1741 gcggggaaag accggtgcac cggccgccgg gttcggtggt ggactgggtt tcgccaatcc
     1801 gaccgacgcc agcacgtcgc gcacgatccc cgcctgcagt gcagcagggg ccagggccag
     1861 agacaggatc actcctatct gaccccaaat aaagcggacc gaagccctcg gttgtacgga
     1921 taggtcgctc atagaaatag gcctcctgcc aaggcggcgt cagcgacgct gcgaatgatg
     1981 ccaccaggtc tgcaatccgc gctacccggc cctaacaggg gggtgacatg tcaccgaatt
     2041 gcaacctgag attaatagag tctcacgcgg cgcacggcgg tgcagcttat aacgatgcgt
     2101 gaacggcgca gggatacgtc acgcaccttc tcccgctcgg tgctcgctcg tcgtgccacg
     2161 cacgatgcgg cgtcgtgcta cgcatccccg gtttgagaga aaacgcggag ccggaagccc
     2221 gtcaaaacga atagcgcttc acgacccact gggagagttc cttgaagacc aatctgaaag
     2281 tcacttacgc cgtggtgctg gcgcttgcga gcgcgcgcgg cgcaatggtt tacgcagcgg
     2341 atgtcgatgc ggacgccgat accggtaaca tgctcggcga ggtggtggtc actgccgagc
     2401 ggcgcaccca gtccatccag gacgtgccga tcacgatcca ggccattacc ggtgaagcac
     2461 tgaagacgct gtcggtcgat acactcgatg acgtcatcaa gtacacgccc aacgtcacgc
     2521 tcggtaccaa cggccccggc caggccgaca tttatatgcg gggtctgagc gccggctcgg
     2581 tcggcggaca gtcaaccgcg acctttgcgt ccttccccaa cgtcgcggtc tacctggatg
     2641 accagtcgct gcagttcccg gcgcgcaacg ccgacgtcta tatggtggac atggagcgca
     2701 tcgaggtgct cgaaggcccg cagggcacac tgttcggtgg tggcgcggaa gccggcgcga
     2761 tccgctacat caccaacaaa cccaagctcg atgccacctc cggcaatgcc gaagccacct
     2821 acggctggac cactcacggt gacaacaaca ccgcgctgaa tgcgacgctg aatgtgccgc
     2881 tgatcgacaa cgcgctgggc gcgcgtctgg tgatctatga cgaccgccgc ggcggttaca
     2941 tcgacaacgt ctatagccag ttcacgcgca gcaacaacga cagcggcaac tactatgccg
     3001 gcatcaagcc gggtgccaat gggctgtgcc ccaacgggca gccgagcacc agcggcttct
     3061 gcgtgccgct caacaacacg gtcggaagca acaccggtct cgcgcgcagt gcgtccaatc
     3121 cggtggaata caacggcctg cgcgcctcgg tactgtggaa gatcagtgac gactgggacg
     3181 cgttgatcac gcagagcttt cagaacctgg aggccgatgg cctctcgacg caatacccga
     3241 tcggttcgaa cggccaggcg ctcggtccgt ggcaggtcac cgagttcgtg cccacctacg
     3301 acaaagatcg ctatgaaaac accgcactga ccgttaacgg caaaatcggc gatctgaagg
     3361 ccgtctacac cggcggctat ctcgaccggc acatcgatca ggtcaacgac tattccaact
     3421 atacccgcag cgcctatggc ttctactaca cctgcactgg cggcccgggc ggtggtggtt
     3481 tcgggactgc gggtaccggt ccgggcgttc cccccggcgg cagcgcgccg gtgtgctatt
     3541 cgccgatcac ctcctggcgc gatcaggtca ccaacacaca cctgagccag gagttccgcg
     3601 tcagcacccc ggatgactgg cggattcgcg gaattctcgg agcgtattac gagaatttca
     3661 aaatccggga cgacatgaat ttctactata agacgatccc agggtgtaac gcgctcaatc
     3721 tgtcggtcgc gctgggcggc ggacccg
//
LOCUS       DATMRM010000287         6365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3241176, whole genome shotgun sequence.
ACCESSION   DATMRM010000287 DATMRM010000000
VERSION     DATMRM010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3241176"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VNZ06_08325"
     CDS             <1..495
                     /locus_tag="VNZ06_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HWW20795.1"
                     /translation="QGRIHTSAVTVAILPELEEIEKVELNPAELRVDTYRSSGAGGQH
                     VNKTDSAIRITHLPTGIVVECQDERSQHKNRSRAMSLLAARLLAAEQEKQIAAQAQSR
                     KLQVGSGDRSERIRTYNFRDGRVTDHRINLTLHQLDKIMEGDIDELLEALQQEHQAEL
                     LAEE"
     gene            complement(468..1205)
                     /locus_tag="VNZ06_08330"
     CDS             complement(468..1205)
                     /locus_tag="VNZ06_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20796.1"
                     /translation="MTASNAPRRGHSVAELLYTAIALFRATLLKCLPFAMVAVLSAEA
                     PNFYWLTTGHRLEKGLPSDPTYWWLAFIFSAVALYIFSAMMLRQVYFSGGFAVNARQE
                     FALAARRLPTLLSAWLLMQVSLFVGLVALVVPAVFLFICYLVLLPVILLEGQLNPVLA
                     LQRCVMLVWPNWWRICAAFVIALITMFVCVLVVAALLEILANLLAGGGSTFQAIFAAG
                     SIAIGAAVFVFFSALALAIHSSANSSA"
     gene            1218..2087
                     /gene="prmC"
                     /locus_tag="VNZ06_08335"
     CDS             1218..2087
                     /gene="prmC"
                     /locus_tag="VNZ06_08335"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWW20797.1"
                     /translation="MAEPCNLADPGADHGAISRWLARGFEALRSSCDSPQLDAELLLA
                     FVLGRARVHVRAHPEQMLDPDAIKRYRELLARRAQGEPLAYLTGEREFWSLTLSVTPD
                     VLVPRPETELAVERCLAVGPQGPCAVADLGTGCGAIALALAHERPQWRLWACDRSEAA
                     LQVARGNARRLKLENIEFVSGEWLAPLKARRFDLIVSNPPYVASGDPALARLRFEPRV
                     ALSPGVTGLEALQHIVHESRSRLLPGGALILEHGTGQGPAVAQTLVSAGYARVRCYAD
                     LAGHDRVTEARWP"
     gene            2105..2599
                     /locus_tag="VNZ06_08340"
     CDS             2105..2599
                     /locus_tag="VNZ06_08340"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWW20798.1"
                     /translation="MVRFDTSHGAFTVDLFAGDAPLSAANFLQYVDDGFYDGTIFHRI
                     VPGFVIQGGGLTKDFDQKKTRAPIKNEATNGERNLRGTLSMARTDDINSATSQFFVNL
                     TDNGFLDHSPGNYGYAVFGRIKDGMDVIDRIAKVKTGRRKHYTDAPMEDVLITSARRV
                     EAPK"
     gene            2653..3345
                     /gene="mtgA"
                     /locus_tag="VNZ06_08345"
     CDS             2653..3345
                     /gene="mtgA"
                     /locus_tag="VNZ06_08345"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HWW20799.1"
                     /translation="MLLLTAALGVLLLVGSVVALRWINPATSAFMIESKLTALRDGDH
                     RYHTEYQWVDLESISPQAALAVIASEDQQFPFHYGFDFHSIREAVRAHERGAKLRGAS
                     TITQQVAKNLFLWSGHSFVRKGLEAGLTLTMETVLPKERILEIYLNVAEFGPGVYGVE
                     AAAGHFFHEHALALTREQSALLAVVLPNPRQLKVNAPSRYVLTQRDRTLQQMAHLGGP
                     AYLRGIESQARE"
     gene            3368..4105
                     /locus_tag="VNZ06_08350"
     CDS             3368..4105
                     /locus_tag="VNZ06_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HWW20800.1"
                     /translation="MHPQSTSVPATGPAHHHRVFKYYDFVMAAYVCVLLCANLIGPAK
                     LSTVQVPLIGNITFLAGVLFFPVSYIFGDILTEVYGYARDRRCVWAGFAALAFAALMA
                     TVIVALPPANNWDGQSAVKAIFGNTWRIVAASIIAFWCGSFVNSLVLAKMKLFTNGRM
                     LWTRIIGSTLCGELVDSALFYTIAFYGLWDSDRVVQVLFTQYLLKSGWEIVMTPVTYR
                     IVAFLKQAEQEDFYDRDTNFTPFSLKA"
     gene            complement(4108..5022)
                     /locus_tag="VNZ06_08355"
     CDS             complement(4108..5022)
                     /locus_tag="VNZ06_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HWW20801.1"
                     /translation="MDEPAVSTGGALRRFIFEHHPVRGFWIRLEGAWRELRSYRQYTP
                     AVEALLGEAVSATLLLAATLKFRGTLTLQLAGDGHVKLLVAQCTHEFEVRAVANTQSE
                     LRGPLNFRELVGDGRLTVTIESEERATRYQGIVSLVGDSLAACLENYFETSEQLPTRL
                     ALVADAARSAGVLIQKMPAASTQGEALAAVSQTVWEEAQSNLQALDHELLRQPDAHAV
                     LESICRDQDCRLFSATAVRFACRCNHTRVEGLLRALGFDEIRSIVAEQGAVTVTCEFC
                     GRPYRFDPIDAERLFSQGSSPDAPPTLN"
     gene            complement(5015..>6365)
                     /gene="gatB"
                     /locus_tag="VNZ06_08360"
     CDS             complement(5015..>6365)
                     /gene="gatB"
                     /locus_tag="VNZ06_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWW20802.1"
                     /translation="QANLVDLAYPGTLPVLNAEAVRMAVKFGLAINARVARRSIFARK
                     NYFFPDLPKGYQISQYELPIVSGGAVEIMLEDGAPKRIGVTRAHLEEDAGKSLHEGLA
                     GQTGVDFNRAGTALIEIVSEPHMRSAKEAVTYLKKIHTLVRYLQICDGNMQEGSFRCD
                     ANVSVRPRGADKFGTRAGIKNLNSFRFVEKAINYEVARQIELLEGGGKVVQETRLYDA
                     DKGETRSMRLKEDANDYRYFPDPDLLPVEIDQPYIDAVCLSLPELPDHKANRFIEQYG
                     LSAYDAGVLTASAELANYYEKVLRIQPEGSSKMLPPKLTANTVIGELASLLNRDGCDI
                     TESRVNEDRLAGLLLRVADNTISGKIAKEVLEAMWDEGRSADEIINARGLRQITDESA
                     IEAAIDAVMAANPSQLADYRAGKEKLFGFFVGQVMKATQGKANPTRLNELLKKKLGG"
BASE COUNT         1182 a         2054 c         1971 g         1158 t
ORIGIN      
        1 caaggacgga tacacacctc ggcggtcacg gtcgcgatac ttccggagct cgaagagatc
       61 gagaaggtag agctcaatcc ggcggagctt cgagtcgaca cctatcgctc ctcgggcgcg
      121 ggcggccagc acgtcaacaa gaccgattcg gccatccgca tcacccacct tcccaccggt
      181 atcgtggtgg aatgccagga cgagcgctca cagcataaga atcgctcgcg cgcgatgtcg
      241 ctgctggcgg cacgcctgct ggcggcggag caggaaaagc agatcgcagc gcaggcgcaa
      301 tcgcgcaaac tccaggtcgg cagcggcgat cgctccgagc ggatccgaac ctacaatttc
      361 cgggatggcc gcgtaaccga tcaccgcatc aatctgacgc tccatcagct cgacaagatc
      421 atggaaggcg acatcgatga gctgctcgaa gcgctgcagc aggagcatca ggccgagctg
      481 ttggcagagg agtgaatcgc cagcgccagc gcactgaaga acacgaacac tgccgcgccg
      541 atggcgatgc tgccggcggc gaagatcgcc tgaaaggtcg atccgccgcc ggccagcagg
      601 ttcgccagaa tctccagtag cgccgccacc accagcacgc agacgaacat cgtgatcagc
      661 gcgatgacga aggccgcgca gatgcgccac cagttcggcc atacgagcat cacgcagcgc
      721 tgcagcgcca gcaccggatt gagctgccct tccagcagga tgaccggcag cagcaccagg
      781 tagcagataa aaagaaacac ggcaggcacc accagcgcga ccagcccgac gaacaagctg
      841 acctgcatca gtagccaggc ggacagcaaa gtgggcagac gcctggccgc gagcgcgaac
      901 tcctggcgtg cattgaccgc aaagccgccc gagaaataca cctgccgcag catcatcgcg
      961 ctgaagatgt aaagcgcgac cgcgctgaaa atgaacgcca gccaccaata cgtggggtcc
     1021 gagggcagcc ctttctcgag cctatggccg gtggtgagcc agtagaagtt gggggcttcc
     1081 gcgctgagta ccgcaaccat cgcgaacggc aagcacttca gcagcgtcgc acgaaacaac
     1141 gcgatggcgg tgtacagcag ctcggcgaca ctgtgaccgc gccgtggcgc gttcgatgcc
     1201 gtcataatgt ctccatcatg gcagaaccgt gcaatctagc agacccgggc gccgaccatg
     1261 gcgcgatcag ccggtggctg gcgcgaggat tcgaggcgct gcgctcatcc tgcgactcac
     1321 cgcaactgga tgccgaactg ctgctggcat tcgtgctggg gcgtgcgcgg gtgcacgttc
     1381 gagcccatcc cgaacagatg ctcgatcctg acgcgatcaa gcgctaccgc gaattgctcg
     1441 cgcgccgcgc gcagggcgaa ccgctggcct acctgaccgg agagcgcgaa ttctggtcgc
     1501 tgacgctgag cgtgacgccg gacgtgctcg ttccgcgccc cgagaccgag ctcgcggttg
     1561 agcgttgtct ggcagtgggc ccgcaagggc cgtgcgccgt ggccgacctc ggtaccggct
     1621 gcggtgcaat cgcgctggcc ctggcgcacg agcggccgca gtggcggctg tgggcctgcg
     1681 atcggtcgga ggcggcatta caggtggccc gtggcaatgc caggcgactg aagctcgaga
     1741 acatcgagtt cgtgtccggc gaatggctcg cgccgctcaa ggcgcgacga tttgatctga
     1801 tcgtgagcaa tccaccgtat gtggcctcgg gcgatccggc gctcgcgcgg ttgcgcttcg
     1861 aacccagggt cgcgctcagc ccaggagtca cgggactgga agctctgcag cacatcgtgc
     1921 atgaatcccg ctctcggctg ctgcccggcg gcgctctgat actggagcac ggcactggac
     1981 agggcccggc ggttgcgcag acgcttgtga gtgcaggcta tgctcgcgta cgctgttacg
     2041 ctgatctggc cggacacgat cgcgtcaccg aggcgcggtg gccttgaact caacggagta
     2101 caccatggtt cgattcgaca cctcgcatgg cgctttcacg gttgatttgt tcgcgggtga
     2161 cgcgccgtta agcgcggcca acttcctgca gtacgtcgat gacggctttt acgacggcac
     2221 gatttttcac cgcatcgttc ctgggttcgt catccagggc ggcggattga ccaaggactt
     2281 cgaccagaag aagacccgtg ccccgatcaa gaacgaagcc accaacggcg agcgcaatct
     2341 gcgcggcacg ctgtcgatgg cccgcaccga cgacatcaac agtgcgacct cgcagttctt
     2401 cgtcaacctg accgacaatg gtttcctcga tcacagcccg ggcaactacg gctatgcggt
     2461 attcggacgc atcaaggacg gcatggacgt gatcgatcgc atcgccaagg tcaagaccgg
     2521 gcggcgcaaa cactacactg acgccccgat ggaggatgtg ctgatcacgt cggcccgtcg
     2581 agtggaggcg ccaaagtagt cacgactgcc tcgcgccgca agtcgcgcgg cctgctctcg
     2641 cggctgggcc gcttgctgct gcttacggca gcgctcggtg tgctgctgtt ggtaggcagt
     2701 gtcgtggcgt tgcgctggat caatcctgcc accagcgctt tcatgatcga gtcgaagctc
     2761 acggcgctac gcgatggcga tcatcgctac cataccgaat accagtgggt ggatctcgag
     2821 tcgatctcac ctcaggccgc gctcgcggtg atcgcttccg aggatcagca gttcccattt
     2881 cactacgggt ttgattttca ttcgatccgt gaggccgtcc gcgcacacga gcgcggcgcg
     2941 aaactgcgcg gcgccagtac catcacgcaa caggtggcga agaacctgtt tctgtggtcc
     3001 ggccacagct tcgtgcgcaa gggcctcgag gccggcctga ccctgacgat ggagacggtg
     3061 ctgcccaagg aacgcatcct ggagatctac ctcaacgtgg ccgaattcgg tcccggggtc
     3121 tatggcgtcg aagccgccgc aggccacttc tttcatgagc acgcgctggc gctcacgcgc
     3181 gagcaaagtg ccttgctggc cgtggtgttg cccaacccgc ggcagttgaa agtgaacgcg
     3241 ccttcgcgct atgtgcttac ccagcgggac cggaccttgc agcagatggc ccatctgggg
     3301 ggcccggcct acctgcgcgg aatcgagtcg caagccaggg aataacgcga acaacaggga
     3361 gagaacgatg catccgcaat ccaccagtgt ccccgcgacg ggcccggcgc accatcatcg
     3421 ggtattcaaa tactacgact tcgtgatggc cgcctatgtg tgcgtcctgt tgtgcgccaa
     3481 cctgatcggt ccggcgaaac tgtctaccgt ccaggtaccg ctgatcggca acatcacctt
     3541 tctggctggt gtactgttct ttcccgtctc atacatcttt ggcgacattc tgacggaagt
     3601 ttacggctac gcgcgcgacc ggcgctgcgt ctgggccggt ttcgcggcgc tggcgtttgc
     3661 cgcgttgatg gcgaccgtga tcgtcgcact gcccccggcc aacaactggg acgggcagtc
     3721 cgcggtgaag gcgatttttg gcaatacctg gcgcattgtc gccgcctcga tcattgcgtt
     3781 ctggtgcggc agcttcgtga acagcttggt actggcaaag atgaaactgt tcacgaacgg
     3841 ccgcatgctc tggacccgca tcatcggctc gacattgtgc ggcgaactgg tcgattccgc
     3901 tctgttctat acgatcgcct tctatggcct gtgggatagc gatcgggtgg tgcaggtgtt
     3961 gttcacgcag tacctgctca agagcggatg ggagatcgtg atgacgccgg tgacctaccg
     4021 catcgtcgcc ttcctgaagc aggccgaaca ggaagacttc tacgaccgcg acaccaactt
     4081 cacgccgttc tcgctgaagg cctgaagtca gttgagcgtc ggcggtgcgt cggggctcga
     4141 gccctgagag aacagccgct cggcgtcaat cggatcgaaa cggtacggac ggccgcagaa
     4201 ctcgcaggtg acggtgaccg cgccctgctc agcgacgatg ctgcggatct catcgaaacc
     4261 caaggcgcgt agcagtccct cgacccgcgt gtgattgcag cggcaggcaa aacgcaccgc
     4321 ggtcgccgaa aacaagcggc aatcctgatc gcggcaaatg ctctccagca ccgcgtgcgc
     4381 gtcaggctga cgcagcagct cgtgatcaag cgcctgcagg ttcgattggg cttcttccca
     4441 gaccgtctgc gagaccgctg ccagcgcctc gccttgcgtg ctggccgccg gcattttctg
     4501 gatcagaacg cccgcgctgc gagccgcatc ggcgaccagc gcgagccgcg tgggcagctg
     4561 ctcggaggtc tcgaagtagt tctcgaggca ggccgcgagg ctgtccccca ccagtgacac
     4621 gatgccctga tagcgcgtcg cgcgctcttc ggattcgatc gtcaccgtca ggcgtccgtc
     4681 gccgaccagc tctcgaaagt tcagcggccc cctgagctct gactgcgtgt tggcgaccgc
     4741 gcgcacttcg aactcatgcg tgcactgcgc gaccagcagt ttcacgtgac cgtcgcccgc
     4801 gagctgcagc gtcagcgtac cccggaattt gagcgtcgcc gccagcagca gcgtcgcgct
     4861 gaccgcctca cctaacagtg cctcgacagc cggtgtgtac tggcggtagc tgcgcagctc
     4921 gcgccacgcg ccctcgagtc gaatccagaa gccgcgcacc gggtggtgtt caaagatgaa
     4981 tcggcgcagc gctcccccgg tgctgacggc cggctcatcc accgagcttc ttcttcagca
     5041 gctcattgag ccgcgtcggg ttggccttgc cctgcgtggc cttcatgacc tgaccgacga
     5101 agaatccgaa cagtttctcc ttgccggcgc ggtagtccgc aagctgcgaa ggattggccg
     5161 ccatcaccgc atcgattgcc gcttcgatcg ccgactcgtc ggtgatctgt cgcaggccgc
     5221 gggcgttgat gatctcatcg gcgctcctgc cctcatccca catggcctcg agcacctcct
     5281 tggcgatctt gcccgagatg gtgttgtcgg ccacgcgcag cagcagtcca gccagacgat
     5341 cctcattcac ccggctttcg gtgatgtcac atccgtcgcg attcaacagg ctggccagct
     5401 cgccgatgac ggtgttggcc gtcaatttgg gcggcagcat cttgctcgac ccttccggct
     5461 ggatgcgcag caccttttcg taatagttcg ccagctccgc gctggcagtg agaacgccgg
     5521 cgtcgtaggc cgacaatcca tactgctcga tgaagcgatt ggccttatgg tcgggcagct
     5581 caggcaggct caggcacacc gcgtcgatgt acggctgatc gatctccacc ggcagcaggt
     5641 ccggatccgg gaagtagcgg tagtcgtttg cgtcttcctt caagcgcatc gagcgcgttt
     5701 cgcccttgtc ggcatcgtac aggcgagtct cctgaaccac cttgccgccg ccctccagca
     5761 gttcgatctg gcgggcgacc tcatagttga tcgccttttc gacgaaccgg aacgagttga
     5821 gattcttgat ccccgcgcgc gtaccgaact tgtcggcgcc gcgtgggcgc accgagacat
     5881 tcgcgtcgca gcggaacgag ccttcctgca tgttgccgtc gcagatctgc agatagcgca
     5941 ccagcgtgtg gattttcttg agataggtaa ccgcttcctt ggccgagcgc atgtgcggct
     6001 cggagacgat ttcgatgagc gccgtgcccg cccgattgaa atccactccg gtctgtccgg
     6061 ccagcccctc gtgcaatgac ttgccggcgt cctcctccag gtgcgcacgc gtcacaccaa
     6121 tgcgcttggg tgcgccatcc tccagcatga tctccaccgc gccaccgctg acgatcggca
     6181 gttcgtactg gctgatctgg tagcccttcg gcagatccgg aaagaagtag ttcttgcgcg
     6241 cgaagatcga gcggcgcgca acgcgcgcgt tgatcgccag gccaaacttc accgccatgc
     6301 gtaccgcttc ggcgttgagc accggcagcg tgcccgggta ggccagatcg accagattgg
     6361 cctgc
//
LOCUS       DATMRM010000288         5264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3242641, whole genome shotgun sequence.
ACCESSION   DATMRM010000288 DATMRM010000000
VERSION     DATMRM010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3242641"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VNZ06_08365"
     CDS             <1..376
                     /locus_tag="VNZ06_08365"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HWW20803.1"
                     /translation="IGGGGGGFNPINTIQRKLGLDRLSIGGGGNASSGGPGAPNALGA
                     APGQNDANAATIEAGRYISSRVYVGAKQSTLGPTQAQVQIDLTKKLKLQATLGNGGGT
                     VQGATPQNDPGSNAGIAYQFEY"
     gene            420..743
                     /locus_tag="VNZ06_08370"
     CDS             420..743
                     /locus_tag="VNZ06_08370"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HWW20804.1"
                     /translation="MDEQVTAEDLYEHLQAVIRDTEALLQATASYAGDKAEQARAKAG
                     DSLRAAKERLSGMQDQVADRARDYLRQGNQYVRDNPWQSVGIAAGVGLLVGALLLGSL
                     SSRRD"
     gene            747..1166
                     /locus_tag="VNZ06_08375"
     CDS             747..1166
                     /locus_tag="VNZ06_08375"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HWW20805.1"
                     /translation="MDYAPQEAQAPPPESRISRFLTSFGQLLVTLVEMTYTRLELIFV
                     ELQEAIQGLVTVLLWGLVAILAAGATLVFAAVALIVAFWDTHRVLVALLVTGSFLLIS
                     LGSLWVMVLRLRSQRALFASTLSEFAKDRELMRRQRE"
     gene            1163..1447
                     /locus_tag="VNZ06_08380"
     CDS             1163..1447
                     /locus_tag="VNZ06_08380"
                     /inference="COORDINATES: protein motif:HMM:NF025363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HWW20806.1"
                     /translation="MTAREPPFVRRRAELQLQCAQQRAAFAQSVEDMQASVQGLNRGL
                     GIVRGTRLMPMLLAALSAAGVLTRAGGVVRLLEKLWVIWQLVQRLRRSKR"
     gene            1444..1914
                     /locus_tag="VNZ06_08385"
     CDS             1444..1914
                     /locus_tag="VNZ06_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWW20807.1"
                     /translation="MKPPSAGILLYRRRQNELQVFLIHMGGPLWAHKDAGAWSIPKGE
                     YGADEEPLQAAQREFHEETGARVSGPFLGLQPIRQRSGKMVSAWATEGEFDPAQLRSN
                     LFTLEWPPGSGRMQQFPEADRGAWFSLAEAGGRIIPGQQGLLAELQSLLSIPPA"
     gene            1934..2098
                     /locus_tag="VNZ06_08390"
     CDS             1934..2098
                     /locus_tag="VNZ06_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20808.1"
                     /translation="MKPQTPNPPPLRENDPPPGKAQKEADQALDRELDRELDDTFPAS
                     DPLPWSHEVK"
     gene            2106..2645
                     /locus_tag="VNZ06_08395"
     CDS             2106..2645
                     /locus_tag="VNZ06_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWW20809.1"
                     /translation="MSVHFFQLSVPVSAVDHVLGPQHAQISVVEYGDFECPNCKQAAA
                     AVKMLLQRFPERVRFVFRNFPLAEVHPHAMQAAEAAECAGAQGQFWPMHDLLFENQLH
                     LKPNHLHGYAERLRIDLARYSAEMDDHVYLQRVREHQQSGRDSGVRGTPTFFVNGRVQ
                     DVSFGMQGLFGAVEAALKR"
     gene            2652..4304
                     /locus_tag="VNZ06_08400"
     CDS             2652..4304
                     /locus_tag="VNZ06_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWW20810.1"
                     /translation="MAHRESILPAATIVLARDEMQLLFLQRNPGASAFAGAFVFPGGT
                     LEEADRDREVWQRVQGLSAEEADRRLNLRDGALSYWVAALRECYEESGILLAVDGGGR
                     ALPAERVAQLAARRDALNRRELTFKDFLAQEDLYIPAGQMVYFAHWITPKVRPRRFDT
                     RFFMIAAPTDQEVQHDNTETVESRWMKPADVLEQVQRNEIKMVHATQLIVAGLGKFTS
                     PAEAVARLRAVEYVPMNRPCVAQGSEGPKFVRHGDAAYAEVLWSDPQESGESLYDLLA
                     DVPKRLDRFVTRVLAPNPGFMTGPGTNSYLVGERELIAIDPGPADPAHIDALLAAADG
                     RLRWIVLTHTHRDHAPGAIALHEATGAPIAGRAARADSGYDVHVPFDRVLQDGDVVQV
                     EGVELQAVHTPGHASNHLCYLLRATGMLFTGDHVVQGSTVVIAPPDGSMNAYLRSLER
                     LKSLDVVILAPGHGYLIGQPQIEAARLIAHRLGREGKVRAALRNAGGSATLEALLPKV
                     YDDVPAAIHPVAARSLRAHLDKLVEDGEVSVSAGVWASRLSP"
     gene            complement(4328..>5264)
                     /locus_tag="VNZ06_08405"
     CDS             complement(4328..>5264)
                     /locus_tag="VNZ06_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW20811.1"
                     /translation="IELQPQALAATGLRTQDVLESIQSAYTGAAVGQTFLGTRTVDTV
                     LLLPDALRHRPEQLAKLMISGPLGEVPLSQVAHLSLGSDRYSIEHDGGQRRVSVTFNV
                     GQGSLQQIVREAQRSIAQSVKLPPGVFIEFTGAAEAERKARSELLMYSGLALALILVV
                     LFMSFRWRSNSWLVMANLPFSLIGSVIAIAITGIGLSLGTVLGLVTVFGVSARNAILQ
                     LAHYEHLVVVEGLAWDAELVVRGANERLVPILMTAAVTAFGLAPLAFGMKQPGQEIEG
                     PMAVTVLGGLLSSTLLNLFVLPAMARRFVRLPARS"
BASE COUNT          936 a         1655 c         1789 g          884 t
ORIGIN      
        1 catcggcggc ggtggcggcg gattcaatcc gatcaacacc atccagcgca agctgggact
       61 ggatcgcctc tcgatcggcg gcggcggcaa cgcctccagc ggcggtcctg gcgcaccgaa
      121 cgcgctcggt gcggcacccg gacagaatga tgccaatgcg gcgacgatcg aggcaggccg
      181 ctatatctcc agccgggtct atgtcggtgc gaaacaatcc acgctcggtc ccacgcaggc
      241 gcaggttcag atcgatctga caaaaaaatt gaagctgcag gcgacactcg gcaacggtgg
      301 cggaacggtc cagggagcca cgccgcagaa cgaccccggc agcaatgccg gcatcgccta
      361 tcagttcgag tattaaagcg cgtaagatct taacggctgt cgaccggagg tacgcgagca
      421 tggacgagca agtcaccgcc gaagatctct acgagcatct ccaggctgtc atccgcgaca
      481 ccgaggcatt gctgcaggcg accgcctcct acgccggcga caaggcggag caggcccggg
      541 ccaaggccgg ggattcgctg cgagcggcca aagagcgtct gtccgggatg caggaccagg
      601 tggccgaccg cgcgcgcgac tacctgcgac agggcaatca gtacgtgcgc gataatccgt
      661 ggcagtcggt gggcatcgcg gccggtgtcg ggctgctggt gggtgcgttg ctgctcggca
      721 gcctgtcgag tcgacgagac tgaaccatgg actacgcgcc gcaggaagcg caggcgccac
      781 cacccgagtc gcgcatcagc cgattcctga catcgttcgg ccagctgctg gtcacgctgg
      841 tggagatgac ctacacccgt ctggagctca tcttcgtcga actccaagag gcgattcagg
      901 gcctggtcac ggtgctcctg tgggggctgg tggcgatcct ggccgccggc gcgaccctgg
      961 tattcgcagc ggtcgcactg atcgtcgcgt tctgggatac gcaccgcgtg ctggtggcac
     1021 tgctggtgac gggctcgttc ctgctgatct cgctcggctc gctctgggtc atggtgctgc
     1081 ggctgcgatc gcagcgggcg ctgtttgcat cgaccctgag tgagtttgcg aaggaccgtg
     1141 aactcatgag gcggcaacgc gaatgactgc gcgcgagcca cccttcgtca ggcgccgcgc
     1201 cgaactgcag ctgcaatgcg cgcagcagcg tgcggcgttt gcacagagtg tcgaagacat
     1261 gcaggcgagc gttcagggtc tgaatcgcgg cctggggatc gtacgcggta cgcggctgat
     1321 gccgatgctg ctcgccgccc tcagtgccgc cggtgtgctg acgcgcgccg gcggcgtggt
     1381 tcgtctgctc gaaaagctct gggtcatctg gcagctggtg cagcgactgc gtcgttcgaa
     1441 gcgatgaaac cacccagcgc cggtatcctg ctgtatcgac gccgccagaa cgagctgcag
     1501 gtcttcctca ttcacatggg cggaccgctc tgggcgcaca aggatgcggg cgcctggtcg
     1561 atcccgaaag gcgagtacgg tgccgatgag gaaccgctgc aggccgcgca gcgcgaattt
     1621 cacgaggaaa ccggcgcgcg ggtgagcggt ccgtttctgg gactgcagcc gatccgtcag
     1681 cgcagcggta aaatggtctc ggcgtgggcc acggaaggcg agttcgatcc cgcgcagctc
     1741 agaagcaatc tgttcacgct cgaatggccg ccgggcagcg gccggatgca acagtttccg
     1801 gaagccgatc gcggcgcctg gttttcgctc gccgaagcgg gcggccgcat cattcccggc
     1861 cagcaggggc tgctggctga gcttcagtcg ctgctgtcga tcccgccggc ctgagtggcg
     1921 ctatagtagt gtcatgaaac cgcagacacc caacccgcca cccctgcgag agaacgatcc
     1981 gcctccgggc aaagctcaga aggaggccga tcaggcgctc gatcgcgagc ttgaccggga
     2041 gctggacgac acctttccgg ccagtgatcc gctgccctgg agtcacgaag tcaagtgagt
     2101 gagtcgtgag tgtgcatttt tttcaactga gcgtaccggt cagtgcggtc gatcatgtgc
     2161 tcgggccgca gcacgcgcag atcagcgtgg tcgagtatgg cgatttcgaa tgccccaact
     2221 gcaagcaggc cgctgcggcc gtgaaaatgc tgctgcagcg attccccgag cgcgtgcgct
     2281 ttgtgttccg gaattttccg ctcgccgaag tgcacccgca tgcgatgcag gctgcggagg
     2341 ccgccgaatg cgccggtgcg cagggacagt tctggccgat gcacgatctg ctgttcgaga
     2401 atcagctgca tctgaagccc aatcacctgc acgggtatgc cgagcggctg cgcatcgatc
     2461 tggcgcgcta cagcgccgag atggacgatc acgtctatct gcagcgggtg cgggaacatc
     2521 agcaaagcgg ccgtgacagc ggagtgcgcg gtacaccgac gttcttcgtc aacggacgag
     2581 tccaggatgt gtccttcggt atgcaggggc tgttcggcgc cgtggaggcc gcgctgaagc
     2641 gttagccatc catggcgcat cgcgaatcga tactcccggc cgcgaccatc gtgctggcgc
     2701 gcgatgagat gcagttgctg ttcctgcagc gcaatcccgg cgcctcggcg ttcgccggtg
     2761 cattcgtctt tcccggtggc acgctcgaag aggccgatcg cgaccgcgaa gtctggcagc
     2821 gtgtgcaggg gctcagtgct gaagaggccg atcgacggct gaatctgcgc gacggtgcgc
     2881 tcagctactg ggtcgcagcc ttgcgtgaat gctacgagga gtcgggcatt ctgctggcgg
     2941 tggacggcgg cggacgggca ttgccggcag agcgtgtcgc gcaactggcg gcgcggcgcg
     3001 atgcgctcaa ccgacgcgag ctgacgttca aggactttct ggcgcaggaa gatctctaca
     3061 tcccggccgg ccagatggtg tacttcgcgc actggatcac gccgaaagtg cgtccgcggc
     3121 gcttcgacac gcgcttcttc atgattgccg cgccgaccga tcaggaagtg cagcacgaca
     3181 acaccgagac ggtggaaagc cgctggatga agccggccga cgtgctcgag caggtgcagc
     3241 gcaacgagat caagatggtg cacgcgacgc agctgatcgt cgccggtctc ggcaagttca
     3301 cctcgcccgc ggaggccgtg gcgcggctgc gcgcggtaga gtacgtgcca atgaaccggc
     3361 catgcgtggc gcagggcagc gagggaccga agttcgtccg ccacggcgat gcggcctatg
     3421 ccgaagtgct gtggagcgac ccgcaggaga gcggcgagtc gctctacgat ctgctggcgg
     3481 atgttcccaa gcgcctcgac cggttcgtca cgcgtgtgct cgctcccaat cccggattca
     3541 tgaccggacc gggcaccaac agctatctgg tcggggagcg tgagctgatt gcgattgatc
     3601 cgggtccggc cgatccggcg cacatcgatg cgctcctcgc ggcggcggac gggcgcctgc
     3661 gctggatcgt gctcacgcac acgcatcgcg atcacgctcc gggcgcgatc gcgctgcatg
     3721 aggcgaccgg tgcgccgatt gccggccgcg cggcgcgcgc cgacagcggc tacgatgtgc
     3781 acgtgccgtt tgatcgcgtg ctgcaagatg gcgatgtggt gcaggtggaa ggtgtggaac
     3841 tgcaggcggt gcacacaccc ggacacgcat ccaatcacct gtgctatctg ctgcgggcaa
     3901 cgggcatgct gttcaccggc gatcacgtgg tgcagggctc gacggtcgtc atcgcgccgc
     3961 ccgacggcag catgaatgcg tacctgcgct cgctcgagcg cctgaagtca ctggatgtgg
     4021 tgattctggc gccaggccat gggtatctca tcggacagcc gcagatcgaa gccgcccgcc
     4081 tgatcgcgca ccgcttgggc cgcgaaggca aagtgcgcgc cgcgctgcgc aacgccggcg
     4141 gcagtgcgac gcttgaggcc ctgctgccga aggtgtacga cgacgtgccg gcggcgattc
     4201 atccggtggc ggcgcgctcg ttgcgcgcac acctggacaa actggtcgag gacggtgagg
     4261 tgagtgtcag tgcgggcgtc tgggcgagcc ggctgtcacc ttaaccgcac cccgtcgcgg
     4321 gcgagcgcta gcttcgcgcc ggcagccgca cgaagcgccg cgccatcgcc ggcagcacga
     4381 acaggttcag cagcgtcgag gacaagagtc caccgagcac ggtcaccgcc atcgggcctt
     4441 caatctcctg tccgggctgc ttcatgccga aggcaagcgg agccaagccg aaggcggtga
     4501 ccgctgcggt catgagtatc ggcaccagac gctcgttggc gccgcgcacg accagttctg
     4561 catcccaggc cagtccttcc accaccacca ggtgctcata gtgcgcgagc tgtagaatcg
     4621 cattgcgggc actgacaccg aacacggtca ccagtccgag taccgtaccc agagacaggc
     4681 cgattccggt gatcgcgatc gcgatcacac ttccgatgag actgaatggc agattggcca
     4741 tcaccagcca actgttggag cgccatcgga acgacatgaa cagcaccacc aggatgagcg
     4801 cgagcgcgag gccggaatac atcagcagct cgctgcgtgc cttgcgttcg gcctccgcgg
     4861 cgccagtgaa ttcgatgaac acgccggggg gcagtttgac cgactgggcg atcgagcgct
     4921 gcgcctcacg cacgatctgc tgcaacgagc cctgacccac attgaaggtg acgctgacgc
     4981 gtcgctggcc gccgtcgtgt tcgatgctgt agcgatccga accgagcgac agatgtgcca
     5041 cctgcgatag cggcacctcg ccgagggggc cgctgatcat gagcttggcg agttgctcgg
     5101 gccgatgccg taaggcatcc ggcagcagca ggaccgtgtc caccgtgcgc gtgccgagga
     5161 aggtctggcc gacggccgcc cccgtgtagg ccgattggat ggattccagc acatcctgtg
     5221 tccgcagtcc cgtggccgcc agcgcctgcg gctgaagctc gatt
//
LOCUS       DATMRM010000289         6612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1736493, whole genome shotgun sequence.
ACCESSION   DATMRM010000289 DATMRM010000000
VERSION     DATMRM010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736493"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VNZ06_08410"
     CDS             <1..816
                     /locus_tag="VNZ06_08410"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HWW20812.1"
                     /translation="RIELAKKGGTWVLPSQFDAPADSARVSSLLDRLSSLKRGLPIAT
                     SDAALKRFKLVDSDFERRVELKFSGKTLDTVYLGDSPGLRKSDARTASDRAVYAVDLP
                     TYELPTDVASWLNADVLHDDVGNLTELDVGSGQSDKVELLKQQGSDKQASSTWTDPAL
                     TGDRHVDSTHAEAFAQQVTQLRVDGVLGTEPKPEWQQDHPALRLELKDDKSHSVDWLL
                     SKPTSGDFYVLKSSLHPWYFSVSSSAAKAMIDASGRDQLIVAAKPQSKGSGKT"
     gene            855..3044
                     /locus_tag="VNZ06_08415"
     CDS             855..3044
                     /locus_tag="VNZ06_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20813.1"
                     /translation="MKIGLLIDHGKAVRIPLLIILAGVALTFWVDQIQELFLLLVSDS
                     SVTERIDVIIMAALLGFAVWHTSRTVYRFDIPTIPTLSDPRAETLREWAPRILGMLVP
                     LLMGIGTWSAQHSTGLRNSSEETSLALTALFFIEAVALLVFFVLRRRVFRKVSSLASR
                     PACDPRVSDWRELPLSVRLVYLSIMALNIVALFVVTYAHGFLAQALTSLGVVLMCASF
                     LTVTGTYLTIQASRWQFPLLSFLLLESLLLQLLNVSDNHHVRLYEGMHSNDAPDPDRV
                     VAAAALAMSFEQYEESWSQAQHSGPVYVVSAEGGGIRAAAWTALVLSELEARSNGEFS
                     KHMLLGSGISGGSLGLALFSVMVSAERRGVLLPKDLPDLARSFNERDFLGATIETMFL
                     TDFLQRFVPGKWFIDRGERLEQGWERAWQEICVKQAAQRTRPPVATGDAVARVCSLFA
                     SQWSALWQPGMPALFLNSTQVDSGERFIQHPFSSIRQGGDILGFFNAATSSAGILPGS
                     SPLSAVVHDSARFTYISPAGTLPDASHLRDKDVLVRQVVDGGYFENSGTTTLAELLEF
                     VIQRKGVLRERCRDTLGRADQCPIRLIHISNDPSVESMRSDDDCGQKPPPPRYGEIRA
                     PLMALVNTRDARSATARAAIRSLFAGDQVQISATDPFTDRTVYHFRLCQGQHHLPLGW
                     TLSPKAYGEMRRQLGVRGDQQGAAPIEPRTGPDRKQLDTILAELSAN"
     gene            3136..4062
                     /gene="ppk2"
                     /locus_tag="VNZ06_08420"
     CDS             3136..4062
                     /gene="ppk2"
                     /locus_tag="VNZ06_08420"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HWW20814.1"
                     /translation="MADLSTLSHDEVIRRIGLDLADAYDEELEMELEDRHPLPDGQAS
                     ESAEDSTKEYRRQYFRQLFRLQSELVKLQDWIVDTRQKVVIVFEGRDAAGKGGAIKRI
                     TQRLNPRVCRVAALPAPNDRERTQWYFQRYATHLPAAGEIVLFDRSWYNRAGVEHVMG
                     FCTPEEYEEFFRSVPEFERMLVRSGIRLLKYWFSITDEEQNLRFLGRIHDPLKQWKLS
                     PMDLESRRRWEDYTRAKEVMLQRSHIPEARWWVVQAVDKKRARLNCIDHLLKQFPYQE
                     VHKESIVLPQRLHHDDYARHPVAAEMLVPEIY"
     gene            4157..5263
                     /locus_tag="VNZ06_08425"
     CDS             4157..5263
                     /locus_tag="VNZ06_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20815.1"
                     /translation="MSPLLSAYFFSGSMKHETAKRILAPIARRFMTGEPADTIDRLFS
                     SKHVNDLRLILAVAFTAVAWIVFFAGLTALIDFADVMHKAHDGALDESIDLLKTAGGD
                     LIKISAPVIGIFGAVLAWAYQVGSNRLGVVDLFACEIDTLCRVCTVTNLVDRQISHYA
                     QIPGTGSVADGEEPANREFSSAENYFPILDSNAPDLQSLEARVVINITAFYTFMKSMR
                     DSLRRLPDVRGESAWHESMSNIIYMLYLALESARHAITDLVEFEPQQAERTMVILLSE
                     LTAYRFLRLQYMDRTDVHNDRLILRGPEYRSLVPELCRLVRSHARGVLDQGNKDQQWA
                     AALQLLPELEKRYDALRQLPLGCGRNPDDTITAI"
     gene            complement(5268..5843)
                     /locus_tag="VNZ06_08430"
     CDS             complement(5268..5843)
                     /locus_tag="VNZ06_08430"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HWW20816.1"
                     /translation="MRRSSWLALYAAAALTSPFAANANNYADAITNFRAAGESRDFFH
                     SAYAYAVFPNVGEGALVVGGQIGKGRVYRNGHLLGYSTIGGLSLGFQAGGQVYSEIVF
                     FEDEHALQEFESGNFQFAAGAHAVAITAGAGASVATNGVQANASGTAQNATTAGAYER
                     GMAVFTVAKGGLIYAAAVGGQHFSYRPRAAS"
     gene            6051..>6612
                     /locus_tag="VNZ06_08435"
     CDS             6051..>6612
                     /locus_tag="VNZ06_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20817.1"
                     /translation="MKKLPILLLACLIPVAALAASPWDGTWKGRLSSMKTTGNPDRFE
                     LNNGLYSCQSCAPPYKIKADGTDQPVPDHAYLDTESVKVVNASTVELTDKKAGKISAW
                     VSYTVSADGKTLSGKFKNYTGEKEVTGSFTETRVAAGPAGSHATSGSWQQQKTSDMSE
                     AALTTTLATTATDLKMSSNGQSIDAKF"
BASE COUNT         1340 a         1945 c         2008 g         1319 t
ORIGIN      
        1 cgcatcgaac tcgcgaagaa aggcggcacc tgggtgcttc ccagccaatt cgatgctccg
       61 gccgatagcg cgcgcgtctc gagtttgctg gacagattgt cgtcgctcaa gcgaggattg
      121 ccgatcgcga ccagcgatgc cgcgctgaag cgcttcaaac tggtcgactc agatttcgaa
      181 cgtcgcgtcg aactgaaatt cagcggcaag acactcgaca cggtctatct tggggattcg
      241 cccgggctgc gcaagtccga cgcccgtacg gccagcgatc gcgcggtgta cgcggtggat
      301 cttccgactt atgagctgcc cacggatgtc gcttcatggc tgaacgcgga tgtgttgcat
      361 gacgacgtcg gcaacctgac cgagctggat gtcggcagcg gccagagcga caaagtcgaa
      421 ctgttgaagc agcagggcag cgacaagcaa gccagcagca cgtggaccga tcctgcgctg
      481 acaggcgaca gacatgtcga tagcacccat gctgaagcgt tcgcgcaaca ggtcacccag
      541 ctgcgggtgg atggcgtgct tgggactgag cccaagccgg agtggcaaca ggatcacccg
      601 gcgttgaggc ttgagctaaa ggatgataaa tcccatagcg tggactggtt gctctcgaaa
      661 cccaccagcg gcgactttta tgtgctgaag agttcgcttc atccctggta cttctcggtg
      721 agttcgtcag cggccaaggc tatgatcgac gcgagtggac gcgaccagct catcgttgcc
      781 gcgaagccac agagcaaagg atcagggaag acctagcgtt tgccatcgat cgacaggaag
      841 tcgcaaaacc aagcatgaag atcggcctgc tcatcgatca cggcaaggcc gtgcgcatcc
      901 cgctgctcat catcttggcc ggtgtcgcgc tgaccttttg ggtagatcaa atacaggagc
      961 tgtttttgct gctcgtatcc gacagcagtg tgacggaacg gatcgatgtg atcatcatgg
     1021 cggcactgct cggctttgcc gtgtggcata cgtcccgcac ggtctatcgg ttcgacatcc
     1081 ctacgatacc gacgctgtcg gatccgcgcg cggaaacgct gcgcgaatgg gcgccgcgca
     1141 ttcttgggat gctggtgcca ctgttgatgg ggatcggaac ctggagcgcg cagcactcga
     1201 cgggattgcg aaactccagt gaagagacgt cgcttgcgct gacggccctg tttttcattg
     1261 aggctgtcgc gctgttggtg ttcttcgtgc ttcgacgccg cgttttccgc aaggtcagtt
     1321 cattggcaag ccgtcccgcg tgcgacccgc gtgtatcgga ctggcgcgag ctgccgctgt
     1381 cggtacgcct ggtgtacctg agcatcatgg ctctgaacat cgtcgcattg tttgtcgtca
     1441 cctacgctca cggctttctc gcgcaggcgc tcacctctct gggtgtggtg ctgatgtgtg
     1501 cctcgtttct caccgtaacc ggtacctacc ttacgattca ggcctcgcgc tggcagtttc
     1561 cgctgctgag cttcctgctg ctcgagtcgc tgctgctgca gctgctgaat gtcagcgaca
     1621 atcatcacgt gaggctctac gagggcatgc actccaatga tgccccggat ccggatcgcg
     1681 tggtggcggc cgctgcgttg gccatgtcct tcgaacaata cgaagagagc tggagccagg
     1741 cgcagcacag cggaccggtc tatgtcgtct ccgcggaggg aggcggcatc cgggctgcgg
     1801 cgtggacagc gctggtattg tcggaactgg aagctcgatc caatggcgag ttctcgaagc
     1861 acatgctgct gggaagcggg atctccggcg gcagtctcgg gctggcgctg ttttcggtga
     1921 tggtcagcgc agagcggcgc ggcgtcctgc tgcccaagga cctgccggac ctggcgcgct
     1981 ctttcaatga gcgtgatttt ctgggtgcaa ccatcgaaac catgttcctc accgactttc
     2041 tgcagcggtt cgtgccaggc aaatggttca ttgaccgtgg cgagcggctg gagcagggct
     2101 gggagcgcgc ctggcaagag atttgcgtca aacaggctgc acagcgaacc cgcccaccgg
     2161 ttgctaccgg cgatgccgtg gcgcgggtct gctctctgtt cgcgagccaa tggtccgctc
     2221 tgtggcagcc tggcatgccg gcgttgtttc tgaattcgac tcaggtggat tcgggtgagc
     2281 ggttcatcca gcacccgttc tcctcgatcc ggcagggcgg ggacattctc ggcttcttca
     2341 acgcggcgac ctcatccgcc ggaatcctgc cgggctccag tccactcagt gccgtcgttc
     2401 atgacagcgc gcgatttacc tatatcagtc cggccggtac cttgccggat gcttcgcacc
     2461 tgcgcgacaa agacgtctta gtcaggcagg tcgtggacgg ggggtatttc gaaaattccg
     2521 gcacgaccac actggccgag ttgctcgagt tcgtgatcca gagaaagggc gtattgcggg
     2581 agcgttgtcg ggacacactg ggacgggcgg accagtgtcc gatccgcctg atccatatca
     2641 gcaatgatcc gtcagtagag tccatgcgca gcgacgatga ctgcggtcag aagccgccgc
     2701 ctccccggta tggcgagatc cgcgcaccat tgatggcgct ggtgaacacc cgcgatgcac
     2761 gctccgccac cgcgcgcgcc gcgatccgca gtctgttcgc aggcgaccag gttcagatat
     2821 ccgccaccga cccgtttacc gaccgcaccg tctatcactt caggctgtgc cagggacagc
     2881 atcacctgcc actcgggtgg acgctctcac ccaaggccta tggcgagatg cgacgacagt
     2941 tgggagtcag gggcgatcag cagggcgcag caccgatcga gccgcgcacg ggcccggacc
     3001 gtaagcaact cgacacgatt cttgccgaac tcagcgcgaa ctgagcgtcg tcttcccgga
     3061 ggttttcccg gttcgcaaca aatctgtcac gcggaccgtg gacgatttgc cggatcgagc
     3121 tcttggaggc acctcatggc agacctttcg accctcagtc acgacgaagt cattcggcgc
     3181 atcggcctgg acctggccga cgcctatgac gaagaactgg agatggagct cgaggaccgc
     3241 catccccttc cggacggcca ggcctccgaa agtgccgagg attcaaccaa ggaataccgt
     3301 cgtcagtact ttcgacagct ctttcgactg cagtccgaat tggtgaagct gcaggactgg
     3361 atcgtcgaca cccgccagaa ggtcgtcatc gtgttcgaag gacgtgacgc tgccggtaag
     3421 ggcggtgcca tcaagcgcat tactcaacgg ctcaatccgc gcgtttgtcg cgtggccgca
     3481 ttgcccgcac cgaatgatcg cgagcgcacc cagtggtatt tccagcgtta cgccacccac
     3541 ctgcccgctg ccggcgagat cgtgctgttc gatcgcagtt ggtacaaccg ggcgggggtt
     3601 gaacatgtga tgggcttctg cactccggaa gagtacgaag aattcttccg ctcggtgccc
     3661 gaattcgagc gcatgttggt gcgatccggc attcgcttgc tgaagtattg gttctcgatt
     3721 accgatgagg aacagaactt acggtttctc ggacgcattc acgatccctt gaaacagtgg
     3781 aagctcagcc cgatggacct cgaatcgcgc cgccgctggg aggactacac ccgagccaag
     3841 gaagtcatgc tgcaacgctc acacattccc gaagcgcgct ggtgggtggt gcaggcggtc
     3901 gacaagaagc gcgcccgcct gaactgcatt gatcatttac tgaagcagtt cccgtatcag
     3961 gaagttcaca aggaatcgat cgtgctgccc cagcgcttac accacgatga ttatgcgcgg
     4021 caccccgtgg ccgcagagat gctggtgccg gagatctact agctgcagtc gattaccttc
     4081 ggtatacgga ttgcgccgac aatagacagc gccggctcat gggaatacaa gtccggaagc
     4141 gataccggag acttgtatgt cccccctcct cagcgcctat ttcttttcag gttcaatgaa
     4201 gcacgagacc gcgaaacgga tactcgcgcc gattgccaga cgtttcatga ctggcgaacc
     4261 tgccgacacg attgaccgcc tgttctcatc gaagcacgtc aatgatctgc ggctgattct
     4321 ggcggtggcg ttcacggcag tggcgtggat cgtattcttt gcggggctga cagcgctcat
     4381 cgatttcgcc gatgtcatgc ataaggcgca cgacggagcg ctcgatgaat cgattgatct
     4441 gctcaagacc gcgggtgggg atctgatcaa gatctcggcg ccggtgatcg ggatattcgg
     4501 cgcagtgctc gcgtgggcat accaggtcgg cagcaatcgg ctgggtgtgg tggatctgtt
     4561 cgcctgtgaa atcgacacgc tttgtcgggt gtgtaccgtc accaatctcg tggaccgaca
     4621 gatttcacac tatgcgcaga tcccgggtac gggcagcgtt gcggacggcg aagaacccgc
     4681 gaaccgcgaa ttcagctcag cggaaaacta tttcccgatc ctggacagca atgcgcctga
     4741 cctgcagagc ctcgaagcga gagtcgtcat caacatcacg gcgttctaca ccttcatgaa
     4801 gtcgatgcgc gattcactgc gcagattacc cgacgtgcgc ggcgaatcgg cgtggcatga
     4861 atcgatgagc aatatcattt atatgctcta tctggcgctt gagagcgcgc gtcatgcgat
     4921 cacggatctg gtggagttcg agccgcagca agccgagcgc acgatggtca tcctgctgag
     4981 cgaactgacg gcataccggt tcctgcgcct gcagtacatg gatcgtaccg acgtgcacaa
     5041 tgaccgactg atcttgaggg gccctgaata ccgttccctg gtgccggagt tgtgccggct
     5101 ggtacgcagc catgcgcgcg gcgtactcga tcagggcaac aaggatcagc aatgggccgc
     5161 cgcgttgcag ctactgcccg agctggagaa gcgctacgac gctttgaggc aactgccgct
     5221 tggctgcggg cgcaatccgg acgatacgat caccgcaatc tgaagcatca ggatgcggcc
     5281 ctcggtcgat aggagaaatg ctgcccgccg accgcagcgg cgtaaatcaa tccgcccttg
     5341 gcgacggtga atacggccat gccgcgctca taagcgccgg cggtggtggc gttctgagcg
     5401 gtgccgctgg cattggcctg gacaccgttg gtggcgacac tggcacccgc gccggcagtg
     5461 atcgcgacag catgagcacc cgccgcgaat tgaaaattgc cgctttcgaa ttcttgtagc
     5521 gcatgctcat cttcgaagaa gacgatttcg ctgtaaacct gcccacctgc ctgaaatccg
     5581 agactcagac cacctatcgt gctgtagccc agcaggtggc cgttccgata cacgcgccct
     5641 ttgccgatct gcccgcccac caccaatgca ccctcaccga cgttcgggaa gacggcatag
     5701 gcgtacgcgg agtgaaagaa gtcacgactt tcgcccgcgg cccggaagtt ggtgatcgcg
     5761 tcagcatagt tgttcgcgtt ggccgcaaag gggctggtca gtgcagccgc tgcgtacaat
     5821 gccagccagg aagagcgcct catactcaat caccttattg gacattcaac atcgcgagta
     5881 ttgcaatgcg tcagcgtgcg taccatagga aatcaggagc gtgcgccgga acgcttctcc
     5941 tacgatgggt gtcagccaac gagaggtttt aacggctcag cacacattgg gtgtaagcta
     6001 cgagccgttt tctacccaca acgaccaata agagaaccaa ggagaggtct gtgaagaaac
     6061 tacctatatt gctattggcc tgcctgattc cggtcgctgc gctcgcggcc agcccgtggg
     6121 acggcacatg gaaggggcgg cttagctcca tgaagaccac gggaaatccc gaccggttcg
     6181 aactcaacaa cgggctgtat tcgtgccaga gttgcgctcc gccctacaag atcaaggccg
     6241 atggtaccga tcagccagta cctgatcatg cctatctcga taccgagtcg gtcaaggtgg
     6301 tgaatgcctc gacggttgaa ctgaccgaca agaaggcggg caagatcagc gcctgggtct
     6361 cctacacggt gtcagccgac ggaaagacgc tgagcggcaa gttcaagaac tacaccggcg
     6421 agaaggaagt caccgggagc tttacggaaa cacgagttgc cgcggggccc gccggttctc
     6481 atgcaacctc cggaagctgg cagcagcaga agaccagcga tatgtcggaa gcagcgctca
     6541 ccacaacgct ggcgacgacc gccacggatt taaagatgag ttcgaatggg cagagcatcg
     6601 atgccaagtt cg
//
LOCUS       DATMRM010000290        11869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3881866, whole genome shotgun sequence.
ACCESSION   DATMRM010000290 DATMRM010000000
VERSION     DATMRM010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11869
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3881866"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VNZ06_08440"
     CDS             complement(<1..1008)
                     /locus_tag="VNZ06_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWW20818.1"
                     /translation="MHEATRTTGDFSAIARREAGLRKTLSAAKLSMIAIGGAIGTGLF
                     LGSGFAIGTAGPAVLISYAIGALIGLLLMGCLAEMTLAHPTTGSFGAYAEHYLGPLAG
                     FMVRYAYWTATVLAVGTEVTAVALYMRFWFPAVPGWCWIVGFSTMLVAVNAFSVSAFG
                     SVEYWFSLIKITAIIVFIMLAAYVISVSAGGTVGFHNYTAYGGFLPHGIGGLWIAVII
                     AVFSYFSIEMIAVAAGEAANPEQAVRSAFRSTIVRLILFYLLTLALMLAVIPWTQAGS
                     SQSPFVLVMQSSHVPSAAAVMNFVVLVAALSAMNSQLYITTRMLFSLSRAGFAPPALG
                     RV"
     gene            1046..2419
                     /locus_tag="VNZ06_08445"
     CDS             1046..2419
                     /locus_tag="VNZ06_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HWW20819.1"
                     /translation="MHGHRDNPGFGILCDAVAYRRLVVVTGSGVSVGLAKPGSDPTTL
                     PTWHSVLTQLQDRFASRLGAVAPDVDLLLSAQPRTSDYLIEAATLIRETVGVGDYRDA
                     VVQLTTAADDARTPLHDLIEDLEPLGIITFNYDLGHENAYRARRHDRHRMHRAIYSDE
                     RKLRAVLAEEFRSRFLLKAHGCISRPKTIVLDRASYREVMARQLGYRAFAHHVLARFS
                     ALIVGFGLNDPDFGDLLQSFEANFGGGVGEHVYIWKRGQREDEEARALVLRRRYGLVC
                     LFVDSFDDVRTVISDARTHMGAELRRTITATLKRSADVEEFRQLRRKAHIELGRLSVA
                     GARVAATALTSVVNDDAEPVLIRAEAAYSLGKIRPTTPGVADFLLGSIRTGTPPPIVT
                     SSLAALLQMDPPVDDQLAIWTARAASLESACAEADRLSGDPESRHPPRARKYLEALIA
                     RWTATSS"
     gene            2524..5040
                     /locus_tag="VNZ06_08450"
     CDS             2524..5040
                     /locus_tag="VNZ06_08450"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HWW20820.1"
                     /translation="MSVEKPLPPTTAELKDSLDTAMVRRLDRLGLAGQDDHWAERYVL
                     CEHLIDPLEADSREQFEATCRFIRDLIAHRWVKTRRAREQAAPKRIHYLSMEFLLGRT
                     LRNNIMNLSAEPLVRHQLDEQKWNLDALIEEEPDAGLGNGGLGRLAACFIDSLATLQY
                     PAIGYGLRYEYGIFKQLIRDGAQLEQPDNWLHCQDPWEIERPGRGYVVPLNATFEMRG
                     NQVNFTRNRPSNLLGMAYDRPVVGYGAKCINTLRLWRAAAPQSFDFAEFSHGDFAGAV
                     IDNVAAESVTRVLYPDDSTEAGRALRFLQQYFLVSCSLQDIIARFTKRGRSNVDWASL
                     PDRIAVQMNDTHPALCVAELMRILLDQARLPWDQAWDLTQRSLAYTNHTLLPEALEKW
                     PVDLFETLIPRQLEIIYEVNRRFLDHVRARFPADERRVQRVSLIEEGSGRRVRMAHLA
                     VVGSHSTNGVAEIHSQLLRTRVLHDFAEIYPERFNNKTNGVTPRRWLQQANPSLSRLI
                     TDTIGKDWVLDMSQVRKLLPLANDAGFRKQFRDAKRAAKVAFADWLRATSHQTVDPDS
                     IFDSQVKRIHEYKRQLLNVLHIVVLYNRARHDPNYAMHPHTFLFAGKAAPAYHFAKLV
                     IKLINAVSATINADPSVRGRLQVLFLPEYNVSLAERLIPASDVSEQISTAGYEASGTG
                     NMKFMMNGALTIGTRDGATIEMAREAGEENFFLFGLTAQQVADSEGWYDPNWHYTHEP
                     ETREALDLIVSDHFSPNDRGVFLPIRRALLDYKDHYRHLADLTDYARAHRELDATYHD
                     PQTWSRKAIINVACSDKFSSDRTIREYADEIWGLKTSTVS"
     gene            5056..6006
                     /locus_tag="VNZ06_08455"
     CDS             5056..6006
                     /locus_tag="VNZ06_08455"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HWW20821.1"
                     /translation="MSKGLLLVDLFSHGDLQVYVDAIRGEKLALQVETADTRPDALTK
                     ARDCSILAILDPAIDQTLIDHMPRLEWIHALTSGTNRITSLRYARRPLISNSSGIHGP
                     QMAELTLLHMLSAARDFPKMLVNAAAARWQRWPQMLLAGKRVLILGVGKIAEALAVRC
                     TALEMTVVGMSGSRTEAPGFECIVPREQVGMQAREADFVVVLLPYDPANHLFVNEAFL
                     QQMKQGAILIGVSRGQVVDEQALLRALESGHLRHAGLDVFAVEPLPANSPLWKSPHLT
                     LTPHVGGMSDRYALQALPILLHNLKAFLGGQPGEMLNRVP"
     gene            6118..6312
                     /locus_tag="VNZ06_08460"
     CDS             6118..6312
                     /locus_tag="VNZ06_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20822.1"
                     /translation="MHKCSKLALAVGAVVALMNLSGCIVAERDHDHGPYTWEHGDRID
                     HYGHREAGWCDRHHEDEHCR"
     gene            complement(6325..6789)
                     /locus_tag="VNZ06_08465"
     CDS             complement(6325..6789)
                     /locus_tag="VNZ06_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HWW20823.1"
                     /translation="MSRAFVRETEAEVIELPDRPISPHPNFVTLSGLATIDARVHALN
                     AERDAAVAAEDQATLPGIDRELRYWRQRRDSARVVEPSPDPKVVRFGVSVRLQFDDGS
                     GRDFRLVGEDEADPAAGLLSWTSPVAKALIGRRAGDILQVFGREAEIVGLRA"
     gene            complement(6870..7250)
                     /locus_tag="VNZ06_08470"
     CDS             complement(6870..7250)
                     /locus_tag="VNZ06_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20824.1"
                     /translation="MRGLFTPILYAGVLSAALLQASCSDPPVLRSRADAATATATNSN
                     DPLAAQCQQLRDQIRANQESQREAASFSTNPQIVAAAQAKADQRIERLHERLDELDCA
                     DQDKQDSSNVRIAPLPPAPNAPNP"
     gene            complement(7343..8269)
                     /locus_tag="VNZ06_08475"
     CDS             complement(7343..8269)
                     /locus_tag="VNZ06_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWW20825.1"
                     /translation="MNATKRQSTKTFSLYGMLPACALATLLTACATTPPEDPQLMAAR
                     SAVDQLEADPLAQQTAGKPLQDARDALAAAESAARERKPQAEIDHLAYLAKRRADIGQ
                     AVVDENRARQQMAQAQSQRDAVLLAARERETQVAREQAASAIAQAEISQQQARSAQAD
                     AQASAAQAQAAQEELNSLQAKQTERGMVLTLGSNLLFDTGSDVLKPGADDSLNRVAQF
                     LQQHHSIEVRIEGHTDSQGSDSYNDGLSERRAGAVAHALESRGVDSGRVQAVGRGKAL
                     PIATNETAAGRQQNRRVEIIFSDLSGQFVSAR"
     gene            complement(8302..8736)
                     /locus_tag="VNZ06_08480"
     CDS             complement(8302..8736)
                     /locus_tag="VNZ06_08480"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HWW20826.1"
                     /translation="MSNTSSAHLLAASAALLALAGCVTSPRQLPEVAAARALISQAEQ
                     SDASQFASADLESARSKLRQADQDAYDNQPVMAAHRAQESSADAEVAMARTRAIKAEQ
                     ALAEVNAGTQTLHNETRRQDPPTSSVIVAPADSTTVGQPVQR"
     gene            8915..8998
                     /locus_tag="VNZ06_08485"
     tRNA            8915..8998
                     /locus_tag="VNZ06_08485"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8948..8950,aa:Leu,seq:tag)
     gene            9055..10344
                     /gene="tig"
                     /locus_tag="VNZ06_08490"
     CDS             9055..10344
                     /gene="tig"
                     /locus_tag="VNZ06_08490"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HWW20827.1"
                     /translation="MTTTKGLERRLEVAVPGERVASEVDQRLKQLARTVRLKGFRPGK
                     VPYAVVQKQFGSQVRAEAINELMQSTFAEAVSQQRVRPATGPRIEPITIDPGSELRYA
                     AVFEVMPEVTLKPLDSIAIERPSAAVTEEDIEAMLQSMRTQRPNFAVVERAARENDRV
                     TIDYEGRIDGSVFPGGKGDAMQVIVGAHRILPEIEQALAGMKAGETKTIPARFPDDYA
                     ATTVAGKQAEFDLKVVSVEEQSLPAIDEEFVRSFGVAAGGVPELRVEVRASMQRELEG
                     AIRTKVRDQLFDALYRDNPMEVPQSMIENQIREMQQQLMRRSGVQDPKQIPAREAFEP
                     GARRRVTLGLLVGEIVRTQNLKVDRARVEEKLEGISASHPDPAEARRQYLASREAMEQ
                     LEAAALEDQAVDWAIGQVKVIDKPSTFRTLTGFGQTT"
     gene            10364..10990
                     /gene="clpP"
                     /locus_tag="VNZ06_08495"
     CDS             10364..10990
                     /gene="clpP"
                     /locus_tag="VNZ06_08495"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HWW20828.1"
                     /translation="MNERGLNLVPIVVEQTSRGERSYDIYSRLLKDRVVFVVGPVDDY
                     MANVVVAQLLFLESENPDKDIALYINSPGGVVSAGLAIYDTMQFIKSDVSTICIGQAA
                     SMGSVLLCGGAKGKRFALPHSRIMIHQPLGGFQGQATDMEIHLKEILDTRERLNRIYS
                     KHTGQTVEKIKTDTDRDNFMDGKDAIAYGLIDQILEKRLELAPAPVKA"
     gene            11108..>11869
                     /gene="clpX"
                     /locus_tag="VNZ06_08500"
     CDS             11108..>11869
                     /gene="clpX"
                     /locus_tag="VNZ06_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003654846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWW20829.1"
                     /translation="MSDDPRGRTDDGKLLYCSFCGKSQHEVRKLIAGPSVFVCDECVE
                     LCNDIIREELEDRADRARDKLPKPHEIRKVLDEYVIGQDRAKKVLSVAVYNHYKRLQT
                     RGSARGKDEVELAKSNILLIGPTGCGKTLLAETLARLLNVPFTIADATTLTEAGYVGE
                     DVENIIQKLLQKCDYDVEKAQTGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKL
                     IEGTIASVPPQGGRKHPQQEFLQVDTSNILFICGGA"
BASE COUNT         2206 a         3663 c         3848 g         2152 t
ORIGIN      
        1 gactcgaccc aaagccggcg gcgcaaatcc cgcgcgtgac aggctgaaca gcatgcgcgt
       61 ggtgatgtag agctggctgt tcatggccga cagggccgcc accaggacca caaaattcat
      121 gacggccgcg gcggacggca cgtgcgagga ctgcatcacc aggacgaacg ggctttgtga
      181 ggagccggcc tgcgtccacg ggatcactgc cagcatcagc gccagggtca gcagataaaa
      241 aagaatcagg cgcacgatgg tcgagcgaaa cgcacttctc acggcctgct ccgggttcgc
      301 cgcctcgccg gcggccaccg caatcatctc aatgctgaag tagctgaata ccgcgatgat
      361 cacggcgatc cacaggccgc cgataccatg cggcaagaat ccgccgtagg cggtgtagtt
      421 gtgaaagccg accgtgccgc ccgcagatac cgagatcaca taggctgcca gcatgatgaa
      481 gacaatgatc gcggtgatct tgatgagtga gaaccagtac tcgaccgagc cgaaggcgct
      541 gacgctgaac gcattcaccg cgaccagcat cgtggagaag ccgacgatcc agcaccagcc
      601 gggcacggcg ggaaaccaga atctcatgta cagcgctacc gcggtgacct cggttcccac
      661 cgccagcacc gtcgcggtcc agtaggcgta gcgcaccatg aagccagcca gcgggccgag
      721 gtaatgttcg gcgtaggcac cgaatgagcc ggtggtcgga tgtgccagcg tcatctcggc
      781 gagacatccc atcagcaaca ggccaatgag ggcgccgatc gcatagctga tcagcacagc
      841 gggtcccgct gtgccgatgg caaaaccgct gcccagaaac agcccggtcc caatcgcgcc
      901 gccaatcgcg atcatcgaca gcttggcggc gctgagtgtc tttcgcagtc ctgcttcgcg
      961 gcgggcgatg gcggagaagt cgcccgtcgt acgtgtggcc tcgtgcacgc cgtcattcta
     1021 tcctgatcaa gtagactgct gccgtatgca cggccatcgg gacaatccgg gatttggcat
     1081 tctgtgtgat gccgtggcct atcggcgttt ggtggttgtc accggcagtg gcgtgtcggt
     1141 cggtctggcg aagccgggct cggatccgac gaccttgccg acctggcatt cggtcctcac
     1201 gcagctgcag gatcgattcg cctcccggct gggggcggtg gccccggacg tggatctgct
     1261 gctgagcgcg cagccacgca cttccgacta cctgatcgag gcggcaacgc tgatccgcga
     1321 gaccgtcggg gtcggggact atcgggacgc cgtcgttcag ctcacgaccg cggcggacga
     1381 tgcgcgcacc ccgctgcacg atctgatcga ggatctggag ccgctgggca tcatcacctt
     1441 caactacgat ctcgggcatg agaatgcgta tcgcgcgcgc cgccatgacc ggcatcgcat
     1501 gcatcgggcc atctattccg atgagcgcaa gctccgggcg gtgctggcgg aggagtttcg
     1561 atcgcgcttc ctgctgaagg cgcatggctg catctcgcga ccgaagacca ttgttctcga
     1621 tcgcgcctcg tatcgcgagg tgatggcgcg gcaactcggc tatcgcgcct ttgcgcacca
     1681 tgtgctggca cgcttcagcg cgctgatcgt gggattcggt ctcaacgatc cggacttcgg
     1741 cgacctgctg cagagcttcg aggcgaactt tggcggtggc gtcggcgaac acgtctatat
     1801 ctggaagcgt ggtcagcgcg aggacgagga ggcacgcgca ctggtgctgc gccggcgcta
     1861 cggtctggta tgcctgttcg ttgacagctt cgatgacgtg cgcaccgtga tcagtgatgc
     1921 gcgcacgcac atgggggcgg agctgcgtcg cacgatcacg gcaacgctca agcgcagtgc
     1981 cgatgtcgag gagttccgcc agctgcggcg caaggcacac atcgagctcg ggcgcctgag
     2041 cgtcgcgggc gcgcgcgtgg ccgccacggc gctcacctcg gtggtcaacg acgatgccga
     2101 gccggtgctg atacgcgccg aggccgccta ttcgctcggc aagatccgtc cgaccacccc
     2161 gggggtagcc gattttctgc tcgggagcat ccgcacgggt acgccgccgc ccatcgtcac
     2221 atcctccctg gctgcgctgc tgcagatgga cccgccggtg gatgatcagc tggcgatctg
     2281 gacagcgcgg gcggcctccc tggagtcggc ctgcgcagag gcggatcggc tgtcggggga
     2341 tcccgagtcg cgccacccgc cccgcgcgcg gaaatacctc gaagccctga ttgcccgatg
     2401 gaccgccacc agctcctaaa gagcgtctgt tcctgtagta cagttgcaac ctcaggcagt
     2461 gacgattcgt tagcatttca tcactccagc ccgatccagg atatcggcca aaggagatag
     2521 cggatgagcg ttgagaaacc cctgccgccc acgacggctg agctcaagga ttcgctcgat
     2581 acggcgatgg tgcgtcgcct ggatcgtctg gggttggccg gtcaggacga ccactgggcc
     2641 gaacgctacg tgctgtgcga gcacctgatc gacccgctgg aagcggattc acgcgaacag
     2701 tttgaggcta cctgccgttt cattcgcgat ctgatcgcgc atcgctgggt gaagacgcgc
     2761 cgcgcgcgcg agcaggccgc acccaagcga attcactatc tgtcgatgga gtttctgctc
     2821 ggtcgcacgt tgcgcaacaa catcatgaat ctgtccgccg agccgctggt gcgtcatcag
     2881 ctcgacgagc agaagtggaa tctcgacgcg ttgatagaag aggaaccgga tgcgggcctg
     2941 ggcaacggtg gcctgggtcg tctggccgcc tgtttcatcg attcactcgc gaccttgcag
     3001 tatccggcca tcggctatgg actgcgctac gaatatggca ttttcaaaca actgatccgc
     3061 gacggcgcac agttagagca gcccgacaac tggctgcact gccaggatcc atgggaaatc
     3121 gagcgtcccg gccgcggcta cgtcgtaccg ctgaatgcga ccttcgagat gcgcggcaat
     3181 caggtcaatt tcacgcgcaa ccggccgtcc aatctgctgg gcatggctta cgaccggccg
     3241 gtcgtcggct atggcgccaa gtgcatcaac acgctgcgtc tgtggcgcgc cgccgcgccg
     3301 cagtcgttcg acttcgccga attctcgcac ggtgatttcg ccggggcggt gatcgacaac
     3361 gtggccgccg agtcggtgac ccgcgtgctg tatccggatg attcgaccga agcggggcgc
     3421 gcgctgcggt tcctgcagca gtacttcctc gtgagctgct cgttgcagga catcatcgcg
     3481 cgcttcacca agcgtggacg ctcgaacgta gactgggcca gtctgccgga tcgcatcgca
     3541 gtgcagatga atgatacgca tccggctctg tgcgtcgctg agctgatgcg gatcctgctc
     3601 gaccaggcgc gccttccctg ggatcaggcc tgggacctca cgcagcgctc gttggcctac
     3661 accaatcaca cactgctgcc ggaagcactg gaaaagtggc cggtagatct gttcgaaacg
     3721 ctgattcccc ggcagctgga gatcatctac gaggtcaatc gtcgctttct ggaccatgtc
     3781 cgggcacgtt ttcccgcaga tgagcggcgc gtgcagcgcg tcagcctgat cgaggagggg
     3841 tccggccggc gcgtgcgcat ggcgcacctg gcggtggtgg gctcccacag caccaacggg
     3901 gtagcggaga ttcattcgca gctgctgcgc acgcgcgtgc tgcacgactt cgcagagatc
     3961 tatcccgaac gtttcaacaa caagaccaac ggtgtgacgc cgcgccgctg gctgcagcaa
     4021 gccaatccgt cgctgtcgcg cctgatcacc gacacgatcg gcaaggattg ggtgctggat
     4081 atgtcgcagg tgcgcaaact gctgccactg gcgaatgatg ccggattccg caagcagttc
     4141 cgcgacgcca aacgcgcggc gaaagtcgcg tttgccgact ggctgcgcgc gacaagccac
     4201 cagacggtcg atccggactc gatcttcgac agccaggtca agcgcatcca cgagtacaag
     4261 cgccagctgc tgaatgtgct gcacatcgtg gtgctgtaca atcgcgcgcg ccacgacccc
     4321 aactacgcga tgcatccgca cacgttcttg ttcgcgggta aggccgctcc ggcgtaccac
     4381 ttcgccaagc tggtcatcaa gctgatcaac gccgtgtcgg ccaccatcaa tgccgatcca
     4441 tcggtgcggg gacgcctgca ggtgctgttc ctgcccgaat acaacgtcag tctggccgag
     4501 cgcctgatcc cggccagcga cgtgtcggaa cagatctcca cggctggcta cgaggcgagc
     4561 ggcaccggca acatgaagtt catgatgaac ggcgcgctca ccatcggaac ccgcgatggc
     4621 gccaccatcg agatggcgcg cgaggcgggc gaggagaact tcttcctgtt tggtctgacc
     4681 gcccaacagg tggccgacag cgagggctgg tacgatccga actggcacta cacccacgag
     4741 cccgagacac gcgaggcgct ggatctgatc gtgtcggatc acttcagtcc gaacgatcgc
     4801 ggcgtgttcc tgccgatccg gcgcgcgctg ctcgactaca aggatcacta ccgacatctg
     4861 gcggatctga ccgactacgc gcgagcgcat cgtgagctgg atgcgactta ccatgacccg
     4921 cagacctggt cgcgcaaggc catcatcaac gtggcctgct cggacaagtt ctcgagcgat
     4981 cgcacgatcc gggaatacgc ggacgagatc tggggcctga agacatcaac cgtcagctag
     5041 tgtcaaactg ggcggatgtc caagggattg ctgctggtcg atcttttcag tcacggggac
     5101 cttcaggtct acgttgacgc gattcgcggc gagaagctgg cgctgcaggt cgagacggcc
     5161 gacacgcgcc cggatgcgct gaccaaggct cgcgactgtt ccatcctcgc cattctggat
     5221 ccggcgattg atcagaccct gatcgatcat atgccgcggc tcgagtggat ccatgcactg
     5281 accagcggca ccaatcgcat cacgagcctg aggtacgcgc ggcgcccatt gatcagcaat
     5341 tccagcggca ttcacggtcc gcagatggcg gaactgacgt tgctgcacat gctctcggcg
     5401 gcgcgcgatt ttccgaagat gctggtcaac gcggccgcag cacgctggca gcgctggcca
     5461 cagatgctgc tggcgggcaa gcgcgtactg attctggggg tcgggaagat cgctgaggcc
     5521 ttggctgtgc gttgcacggc cctcgagatg acggtggtcg gtatgagcgg ctcgcgcacc
     5581 gaggcacctg gattcgagtg tatcgtgcca cgcgagcagg tcgggatgca ggcgcgggaa
     5641 gcggacttcg tcgtcgtcct gctgccgtat gatccggcca atcatctgtt cgtcaatgag
     5701 gctttcctgc agcaaatgaa gcagggggcc atattgatcg gcgtatcgcg cggccaggtg
     5761 gtggatgaac aggcactgct tcgggccctg gaaagcgggc atctgcgcca cgccgggctg
     5821 gacgtattcg cggtcgagcc gctgccggcc aacagcccgc tctggaagtc tccacatctc
     5881 acgctgacac cgcacgtcgg tggcatgagt gaccgctatg cgctgcaggc tctgccgatc
     5941 ctgctgcaca atctcaaggc gttcctgggc gggcaacccg gcgaaatgct caaccgggtg
     6001 ccctgacgag tgcaggtttt ttccgaaatc acctacatcc gaacctcgtg acgtcttgcc
     6061 ggctttctgc aagcgaggcc gtagcatccg cccatgcgtt tcacatggag agggtcaatg
     6121 cacaaatgca gcaagctggc actggcggtg ggtgccgtgg tcgctctcat gaatctgagc
     6181 gggtgcatcg tcgccgagcg cgaccacgat cacggtccgt atacctggga gcatggtgac
     6241 cggatcgatc attacgggca tcgggaagcc ggctggtgcg atcgccacca cgaggatgag
     6301 cactgccgtt gagagcggcc ggaactaggc gcgcagcccg acgatttccg cctcgcggcc
     6361 aaatacctgc aggatgtcgc ctgcacggcg tccgatcagg gccttggcga ccggcgaggt
     6421 ccagctcagc aggccggcgg ccggatcggc ttcatcttcg ccaaccagac ggaaatcacg
     6481 cccgctgcca tcgtcgaatt gcagccgcac gctgacaccg aagcgcacca ccttcggatc
     6541 cggggatggc tcaaccaccc gcgcgctgtc gcggcgctga cgccagtagc gcagctcccg
     6601 atcaatgccg ggcagggtcg cctgatcctc cgcagccacc gcggcgtcgc gctcggcgtt
     6661 caatgcgtgc acgcgcgcgt cgatcgttgc cagtccgctg agcgtgacga aattcgggtg
     6721 cggactgatc ggccgatccg gcaactcgat cacttctgct tccgtttcgc gtacaaaggc
     6781 tcgactcatg attcaagagt gctctgtttg tgtgcatccg ccaagtccgt cgtgcgcatg
     6841 gctctgtcgg agtgggttga ccgtgatcct taagggttcg gagcgttcgg cgcgggagga
     6901 agaggggcaa tgcgcacatt ggaagaatcc tgcttgtcct gatcggcgca gtccagttca
     6961 tccaggcgtt cgtgcagtcg ctcgatgcgc tgatcggctt tggcctgggc ggcggcgacg
     7021 atctgtggat tggtgctgaa gctagccgct tcgcgctggg attcctgatt ggcgcggatc
     7081 tgatcgcgca gctgctgaca ctgcgcggcg agcggatcat tgctgttggt cgcagtggcg
     7141 gtggcggcgt ccgcgcgact gcgcagcacc ggcggatcag aacaggacgc ctgcagcagc
     7201 gccgcactga gtacgcccgc gtacaggatc ggcgtgaaga gtcctctcat gattgcctgc
     7261 cattattgct ctgccacaag cggccggcta tagctgcaag gtatagaccg gccgcttcgc
     7321 ggtcgcgttc cctaaagaac tcttaacgtg cgctcacgaa ctgtccgctc aggtccgaaa
     7381 agatgatctc cacgcgccgg ttctgctgcc tgcctgcggc ggtctcgttg gtggcgatcg
     7441 gcagcgcctt gccgcgtccg accgcctgca cccggccgga atcgacaccg cggctctcca
     7501 gtgcatgggc cacggcaccg gcacgtcgtt cggacaggcc atcgttgtag ctgtccgagc
     7561 cctggctgtc ggtatggccc tcgatgcgca cctcgatgct gtggtgctgc tgcagaaact
     7621 gcgcgacgcg gttcagtgaa tcatcggcac cgggcttcag cacatcgctg ccggtatcaa
     7681 acagcagatt gctgcccagc gtcagcacca tgccgcgttc ggtctgcttc gcctgcaggg
     7741 agttgagttc ctcctgcgcg gcctgtgcct gcgccgcgga ggcctgcgca tcggcctgtg
     7801 cgctgcgcgc ctgttgctgg gaaatttcgg cctgggcgat tgctgatgcc gcctgctcgc
     7861 gtgccacctg cgtctcgcgc tcgcgagctg ccagcaacac ggcatctcgc tgggattgcg
     7921 cctgtgccat ctgctgacgg gcgcgattct catcaaccac cgcctggccg atgtcggcgc
     7981 ggcgcttggc cagataggcg agatgatcga tttcagcctg tggtttgcgc tcacgcgccg
     8041 cgctttccgc cgccgccagt gcatcgcgtg catcctgcag cggtttgcct gcggtctgct
     8101 gcgccaacgg gtcggcttcc agttgatcga ccgcgctgcg cgccgccatc agctggggat
     8161 cctccggtgg tgtggtcgcg cacgcggtca gtagggtggc gagggcacag gccggcagca
     8221 ttccatagag tgagaatgtc ttcgtggact gtcgtttggt ggcgttcacg ggttgggtct
     8281 ccatgtgggt gtcacggcgg ttcagcgctg cacgggctgc ccgaccgtcg ttgaatccgc
     8341 gggcgcgacg atgaccgatg aggtcggcgg atcctgccgg cgagtctcgt tgtgaagcgt
     8401 ctgcgtgccg gcattgacct cggcgagggc ctgctcagcc ttgatcgcgc gcgtgcgggc
     8461 catcgccact tcggcgtcgg ccgaggactc ctgcgcccgg tgtgcggcca tgaccggttg
     8521 gttgtcgtat gcgtcctggt cggcctggcg cagcttgctg cgggccgatt ccagatcggc
     8581 gctggcaaac tgtgaagcat cgctctgctc cgcctgcgaa atcagcgcgc gggcggctgc
     8641 gacctccggt agctggcggg gagaggtgac acagccggcc agggccagca gcgcggccga
     8701 ggctgcgagc agatgagcac tgctggtgtt ggacatgtgc tgtctcctgt tgctgaggtg
     8761 gatagtgtcg gcactgcctg catccccggc tgtaggcgcg gagcctgccg aagcggcgga
     8821 aaagcgaccg cgggtggccg aacgggaggc ccgtggagcg tcggacgact cctatctcgg
     8881 ctataattcg cggcccattt gggtgcgcgt gagcgcgaaa gtggcggaac ggtagacgcg
     8941 ctggccttag gagccagtgg ggcaacccgt gagagttcga gtctctcctt tcgcaccacc
     9001 tactcgatcg ccggttatga caacttcatg gaaagcggca cgatgcaagt atcgatgact
     9061 acgaccaagg gcttggagcg acgcctcgag gtggcagtgc ccggcgagcg cgtggcaagc
     9121 gaagtggacc agcgcctcaa acaactggcg cgcaccgtgc gactgaaagg gtttcgcccc
     9181 ggaaaagtac cgtacgcggt ggtgcaaaag cagttcggca gccaggtccg cgccgaggcg
     9241 atcaatgagc tgatgcaaag cacattcgcc gaagcggtca gccagcagcg cgtgcgtccg
     9301 gcgaccggac cgcgcataga gccgatcacg atcgatccgg gcagcgagct gcgctacgcg
     9361 gcggtttttg aagtgatgcc ggaggtcact ctcaagccat tggacagcat cgcgatcgag
     9421 cggccgagcg ccgcggtgac cgaagaggac atcgaggcga tgcttcagag catgcgcacc
     9481 cagcgaccga atttcgcggt ggtcgaacgc gcggcgcgcg agaacgatcg tgtgaccatc
     9541 gactatgaag gacgcatcga tggcagtgtg tttccgggcg gcaagggcga tgccatgcag
     9601 gtcattgtcg gcgcgcatcg cattttgccg gagatcgaac aggcgctggc cggcatgaag
     9661 gccggcgaga ccaagaccat cccggccagg ttccctgacg attacgctgc caccaccgtc
     9721 gccggtaagc aggccgaatt cgacctgaag gtcgtcagcg tcgaggagca gtcgctgcca
     9781 gcgatcgatg aggaattcgt gcgctccttc ggcgtagccg cgggtggcgt accggaactg
     9841 cgcgtggaag tgcgcgccag catgcagcgt gagctcgaag gcgcgatccg caccaaggtg
     9901 cgcgatcagc tgtttgacgc gctctatcgc gacaatccga tggaagtgcc gcagagcatg
     9961 atcgagaacc agatccgcga aatgcagcag cagctgatgc gccgtagcgg cgtgcaggac
    10021 ccgaagcaga tccccgcccg tgaggcattc gagcccgggg cgcgccggcg cgtgacgctc
    10081 ggtcttctgg tcggggagat cgtgcgtacg cagaatctga aagtcgatcg ggcacgcgtc
    10141 gaggagaaac tggagggcat cagcgcctcg catccggatc cggccgaagc gcgccgccag
    10201 tacctggcga gccgcgaggc gatggagcag ctcgaagccg cagcactgga agatcaggct
    10261 gtcgactggg caatcgggca ggtgaaagtg atcgacaagc cgtctacatt ccggacattg
    10321 acaggctttg gccaaaccac ctgaaaggca tacacttaac accatgaacg aacgcggctt
    10381 gaatctggtt ccgatcgtcg tcgagcagac ctcgcgtggc gagcgctcgt acgacattta
    10441 ttcgcgcctg ctcaaggatc gagtggtatt tgtcgtcggc ccggtggacg actacatggc
    10501 caacgtggtg gtcgcgcagc tgctcttcct ggaatccgag aacccggata aggacatcgc
    10561 gctgtacatc aactctcccg gcggggtggt cagcgccggg ctggccattt acgacaccat
    10621 gcagttcatc aagtccgacg tcagcacgat ctgcatcggt caggcggcca gcatgggctc
    10681 ggtattgctg tgcggcggtg ccaagggcaa gcgtttcgcg ctcccgcact cccgcatcat
    10741 gatccaccag cccctgggcg gctttcaggg gcaggccacg gacatggaaa tccatctgaa
    10801 ggagattttg gatacccgcg aacggttaaa tcgcatctat tcaaaacaca ccggccagac
    10861 cgtcgagaag atcaagaccg ataccgatcg ggataatttc atggacggca aggacgcgat
    10921 agcctacggt ctgatcgacc aaattctgga aaagcgcctg gagctggcac cggcgcctgt
    10981 caaggcgtga ccgggcgctc aaaaacgcga aataacaatg tgttgcgagc gacgcagtcc
    11041 tttgcgtcgg tggtcggccc catgctatat agaaccgcat gaagggtgaa acagtgggtg
    11101 ctatccgatg agtgatgatc cccgtgggcg aactgacgat ggcaagctgc tgtactgctc
    11161 gttctgcggc aagagccagc acgaggtacg caagctcatc gccggtccct cggtgttcgt
    11221 ctgcgacgag tgcgtcgagc tgtgcaacga catcattcgc gaggagctgg aggatcgggc
    11281 tgaccgtgcc cgcgacaagc ttcccaagcc gcatgagatc cgtaaggtgc tcgatgaata
    11341 cgtcatcggg caggatcgcg cgaaaaaggt gctctcggta gccgtctaca accactacaa
    11401 gcggctgcag acccgcggca gtgcgcgtgg caaggacgag gtcgaactcg ccaagagcaa
    11461 tatcctgttg atcggtccga ccggctgtgg caagacgctg ctggccgaga ccctggcgcg
    11521 gttgctgaac gtcccgttca ccatcgccga tgccaccacc ctcaccgagg ccggctatgt
    11581 cggcgaggac gtggagaaca tcatccagaa gctgctgcag aagtgcgatt acgacgtcga
    11641 gaaggcccag accggcatcg tctatatcga cgagatcgac aagatttcgc gcaagtccga
    11701 taacccctcg atcacgcgtg acgtgtccgg cgagggcgtg cagcaggcgc tgctcaaact
    11761 gattgaaggc accatcgcct cggttccgcc tcagggtggt cgcaagcatc cgcagcagga
    11821 atttctgcag gtggacacct cgaatatcct gttcatctgc ggtggcgcg
//
LOCUS       DATMRM010000291        12810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3555343, whole genome shotgun sequence.
ACCESSION   DATMRM010000291 DATMRM010000000
VERSION     DATMRM010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12810
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3555343"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VNZ06_08505"
     CDS             complement(<1..405)
                     /locus_tag="VNZ06_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HWW20830.1"
                     /translation="MSGALAVFDLDGTITRRDTLGAYLWGYLWRHPWRLLWVIAHLYA
                     PLLFLLDRDRGALKGTAIHAILGGVTRDRIRAWSAQFVRELIPRGLFAEGLVAIAMHR
                     ARGDRLLLMSASTDLYVPDIAQALGFDEVICTE"
     gene            complement(410..3103)
                     /gene="acnA"
                     /locus_tag="VNZ06_08510"
     CDS             complement(410..3103)
                     /gene="acnA"
                     /locus_tag="VNZ06_08510"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HWW20831.1"
                     /translation="MNDSFKTRTTLTSGDHTYDIWSLPALGAEKVSRLPYSLKILLEN
                     LLRFEDGTNVTVKDVEALLNWDPKATPSYEISFTPARVIMQDFTGVPAIVDLAAMREA
                     MTRLGGNADEINPLAPAELVIDHSVQVDSYGTAHALADNNRIEFERNGERYAFLRWGQ
                     SAFRNFKVVPPNTGIVHQVNLEYLGRVVFDKHTGSERHAYPDTVVGTDSHTTMINGLG
                     VLGWGVGGIEAEAAMLGQPVTMLIPQVIGFNLTGSLPAGTTATDLVLTVTEMLRKKGV
                     VDKFVEFFGDGLANLPLADRATIGNMSPEFGSTCAIFPIDEETLRYLKLSGRPPEQIH
                     LVEDYARAQGLWRHDGAKPAQYTDVLQLDLGSVEPSLAGPSRPQDRVPLRVAKSTYAK
                     NHARMAEERARKNPGATGNAQVTLADKSFELKDGAVLIAAITSCTNTSNPYVLIAAGL
                     LARNAHQRGLNSKPWVKTSLAPGSRVVTDYLKKANLLDELASVGFNLVGYGCTTCIGN
                     SGPLKDEISAGVKAGDIIGCSVLSGNRNFDGRVHPEVKMNFLASPPLVVAYALAGSLD
                     VDLNNDPLGHGADGKPVYMRDIWPAPAEVAEYVQKNINSEMFRRSYGSVFTGDERWQG
                     IRVPQGKIYAWDSKSTYVKNPPYFEGMTMTPALVSGIKGARLLALLGDSVTTDHISPA
                     GNIAKNSPAARYLVEQGVQPVDFNQYGARRGNHEVMMRGTFANIRLRNLLLPGTEGGI
                     TEYLPSGEQMSIYDAAMRYKADNTPLIIIAGKEYGTGSSRDWAAKGTMLLGVRAVIAE
                     SFERIHRSNLIGMGVLPLQFKDAQNAHSLKLSGRERFEITGFDGADARTASVIATSEN
                     GEPIKFDLRVRIDTPKEREYFRHGGILQFVLRQLAGQARAA"
     gene            complement(3100..4245)
                     /gene="mnmA"
                     /locus_tag="VNZ06_08515"
     CDS             complement(3100..4245)
                     /gene="mnmA"
                     /locus_tag="VNZ06_08515"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HWW20832.1"
                     /translation="MNPGAPARVIVGMSGGVDSAVAALLLHDAGHDVQGLFMSNWEQD
                     DDYCTTAQDYQDARRVAADLAIPLHRVNFADEYRDRVFQYFLDEYQAGRTPNPDVLCN
                     REIKFGLCLNYAQRLGAQFYATGHYARVQQLSDGPALYRAVDHAKDQSYFLHAVERAR
                     FAQVLFPLAELHKDEVRERARQSGLPVHDKPDSTGICFIGERPFAEFLGRFLQDSPGP
                     IETPLGRVLGQHRGLPFYTLGQRSGLEIGGARGYAQEPWYVARKDSTRNALIVVQRHE
                     QHLLDANAVVCGRINWLCAARTGSFSAQVKLRYRQSAQPARIEAGDDGAALIEFEHPQ
                     RAATPGQFAVLYDADRCLGGGVIEQARYNSEALSRHSNSRAASTEDV"
     gene            complement(4242..4721)
                     /locus_tag="VNZ06_08520"
     CDS             complement(4242..4721)
                     /locus_tag="VNZ06_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWW20833.1"
                     /translation="MVWKPDVTVAAIIERDDRFLLVEELIRGHLVLNQPAGHLEDGES
                     LIQAVVRETLEETAWSFAPSALVGIYLWRSQRAHTTLRFAFTGSVSDFDAERALDPPV
                     QATHWLTRADILQRRAQLRTVLVTRCIDDYLAGQRLPLAAIANEVLNQRVPEQRPSP"
     gene            complement(4781..5383)
                     /locus_tag="VNZ06_08525"
     CDS             complement(4781..5383)
                     /locus_tag="VNZ06_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20834.1"
                     /translation="MTVVTASRTTTSAGLSSFGLEDGPVVSNALSQRPAQKAPVPETL
                     HVLSTFDEVRSATETVAASGQRLISIMTPDLEPEIFDQPALLEVIKRFVLGHSFAKVR
                     VLMRDQARLGNGANRFVAMAHRLTSYLEIRIRAVQYRELTAAYCIADDRAIVYRPRAD
                     RPDGLAGFNNPKVARQYLQEFDTVWLASAPEEREVRIARR"
     gene            complement(5529..6911)
                     /gene="purB"
                     /locus_tag="VNZ06_08530"
     CDS             complement(5529..6911)
                     /gene="purB"
                     /locus_tag="VNZ06_08530"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF006764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HWW20835.1"
                     /translation="MDLSPLNALSPLDGRYASTSEPLRAYFSEGALIRERIRVEALWF
                     RQLSDPALGLLHSPLPAAVDETVSGLVADPGAHAAPQVKAIETRINHDVKSVEYFVRE
                     VLANAGADQRALELVHFGCTSEDINNLSYARLMSAAREQLLLPELTQLLQTLSQLARQ
                     YADLPMLSRTHGQAASPTTFGKEVANVGARLRRARRRLQQVPILAKWNGAVGNYNAHH
                     SARENIDWIGLSETFIRQQQLQWNAYSTQIEPHDWIAEFCDATAAINSILIDWSRDAW
                     GYISLGYLRQRALAGEVGSSTMPHKVNPIDFENAEGNFGLANAMLRFFADKLPLSRWQ
                     RDLTDSTVLRNLGVAMAHSFIGWKALQRGLARISPDPQRMSEDLDEAYETLGEAIQSV
                     LRLAGVPNGYEQLKQFTRGQRIDADSLRTFVATLPLPEGDRQRLAALKPSQYIGLAAH
                     LARRFGTEDE"
     gene            6969..8372
                     /locus_tag="VNZ06_08535"
     CDS             6969..8372
                     /locus_tag="VNZ06_08535"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005107031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HWW20836.1"
                     /translation="MNSTSFRTERDSMGELQVPSDALWGAQTQRAVNNFPISGLTLPR
                     SFIRALALIKQAAAAANVRLGLLERALGEAIVRCAAEVATGAHDRQFPIDIFQTGSGT
                     SSNMNANEVIAHLALRSGTPVHANDHVNMCQSSNDVIPTAIHVSAALMLKEELLPSLQ
                     HLAQVIGAREAELIHIVKTGRTHLMDAMPVTLGQELSAWRTQIENGTARLRAVEPRLL
                     SLAQGGTAVGTGINAHPEFSATVCSELQRLTAIAFKPSSNFFESLAAQDTAVELSGQL
                     RVVAVSLMKIANDLRWMNSGPLAGLGEIALPALQPGSSIMPGKVNPVIAEATAMVAAQ
                     VIGNDATIAVAGQSGSFQLNVMLPLIAYNLLESIRLLSNVSRALADRAIRGFTVNRER
                     LAEAVDRNPILVTALNPVIGYEKGAAIAKKAYAEGKPIRDVAQAQTSLSRKELDQLLD
                     PFELTRGGIHAGGGGGG"
     gene            8386..9060
                     /locus_tag="VNZ06_08540"
     CDS             8386..9060
                     /locus_tag="VNZ06_08540"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HWW20837.1"
                     /translation="MAEAAASISPQLPADWRARLSRGLLTQPDVEQENVRLGGLASHS
                     DSVRARVREAWPENMRAAAVLVGIVDRPEAPTLLMTVRARHLRQHPGQISFPGGRLEP
                     FDADIAAAALRETHEEIGIAVGFIQPLGFLTDHVVQTGFRITPVVAMIRPGFTLNPDS
                     TEVADVFELPLSLALAIESYRAQQRMVRDFKVDVWELPFGEYHIWGATAGILMNLCEV
                     VSRAHA"
     gene            9057..9851
                     /gene="mazG"
                     /locus_tag="VNZ06_08545"
     CDS             9057..9851
                     /gene="mazG"
                     /locus_tag="VNZ06_08545"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HWW20838.1"
                     /translation="MSKAAEELDALLRLMRSLRAPDGCPWDREQTFATIAPFTIEEAY
                     EVADAIGRGDLTRLRDELGDLLFQVVFHARMAEEAGKFDFADVARAICDKLIRRHPHI
                     FAERKQLDVAQLHVSWEAQKALERAEAGHHGVLSDVPRSLPALARAAKLGKRAGRVGF
                     DWDSASGVRAKLDEELGEIDEALKAAHPEAVADEIGDLLFTVANWARHLGVDPESALQ
                     RAALKFETRFERMEHSAGGQNLSLSQLSAAQWEQLWQAAKSLADKH"
     gene            9900..10208
                     /locus_tag="VNZ06_08550"
     CDS             9900..10208
                     /locus_tag="VNZ06_08550"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HWW20839.1"
                     /translation="MESNAADSVKLVRRSAWVAAVILLLGLAGSCVASAVEPANLREI
                     SREQATQELQKRYGTAARVIRTDVIEQGGHHVYVFRLLSANGRVWVVRIDAQSGAEVP
                     "
     gene            10222..10905
                     /locus_tag="VNZ06_08555"
     CDS             10222..10905
                     /locus_tag="VNZ06_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWW20840.1"
                     /translation="MRVLVVEDESALREALKVELSAAGYTVDLAADGEEGLYAATEYP
                     IDVAIIDLGLPKMSGLELIRQLRAKGKSYGVLILTARDRWQDKVEGLSAGADDYVPKP
                     FHFAEVQARLAALLRRAGGWASPTLACGPVELDTRAQTVSVDGKAVELTSFEYRILEH
                     LMLRAGEVISKTELTERLYDQDFDRDSNVVEVLVGRLRRKLDPDDALKPIETLRGRGY
                     RLSLPRRCA"
     gene            10941..12317
                     /locus_tag="VNZ06_08560"
     CDS             10941..12317
                     /locus_tag="VNZ06_08560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW20841.1"
                     /translation="MMRSPPSVSRRLLIAVAVPLLLFFVLTGWVIDHVFQQQSEEALR
                     ARLEEQVVALVTAVDLDRDGNLMVNQLDPEHWLEVPGSGQYASLRDEKGKLIWNSPSL
                     SGTGLELGSKLPQGSESFRSLLARDGSSVAELSRRLHLDLGGVKENLLFTAADSTAPQ
                     VRQLRKFRQRMAEWFGALALALLATMAWMMRRALAPVRRLEQEIAAVELGAAQSLGDA
                     YPRELAGVTEGLNALLESERNRIARYRDTLGNLAHSLKTPLAVIRASLDNVQSDAART
                     QSTIENEIERMAQIVAHQLKRAAAGGGATLGQAPLPVLPLVTDLRSAMLKVHARKDLR
                     IDIDVPPEPGFLGDSGDILELLGNLVDNACKWCRSRVMVSASLDPARELPRRLSIVVE
                     DDGPGIAPENRTRVIERGVRADEHVPGHGLGLAMVRETAGLYGGRFFIDESVALGGAR
                     VELQLPGR"
     gene            complement(12314..>12810)
                     /locus_tag="VNZ06_08565"
     CDS             complement(12314..>12810)
                     /locus_tag="VNZ06_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWW20842.1"
                     /translation="DNAVRAFERAVAAAQAAGGEMLVGGNVLQRPGHYVEPAIVVAQN
                     DWEIVQQETFGPILYLITVDSLEQAIALQNTSAYGLSSSLFTQSLRNAETWLSHRGSD
                     CGIANVNIGTSGAEIGGAFGGEKDTGGGREAGSDAWKAYMRRQTNTINWSNALPLAQG
                     IEFG"
BASE COUNT         2202 a         4008 c         4303 g         2297 t
ORIGIN      
        1 ttcggtgcag atcacttcat cgaaacccag ggcctgcgcg atatccggca catacagatc
       61 ggtgctggcc gacatcaaca gcagccggtc gccccgggcc cggtgcatgg cgatggccac
      121 cagtccttca gcaaacaggc cacggggaat cagttcgcgc acgaactgcg ctgaccacgc
      181 ccggatgcga tcgcgggtga caccgcccag gatcgcgtgg attgccgtgc ccttgagtgc
      241 gccgcggtcg cgatcgagca ggaacagcaa cggtgcgtac aggtgggcaa tcacccacag
      301 caatcgccac ggatgacgcc acagataacc ccacaggtac gcgcccagcg tatcgcgtcg
      361 cgtgatcgtg ccgtcgagat cgaagaccgc gagcgcgccg ctcaaggctt caggccgcgc
      421 gcgcctgtcc cgcgagctga cgcaacacga actgcaggat gccgccgtgg cggaagtact
      481 cgcgctcctt cggcgtatcg atgcggacgc gtaggtcgaa cttgatgggc tcgccgttct
      541 cgctcgtcgc gatcaccgag gcggtgcgcg catcggcgcc atcaaagccc gtgatctcga
      601 agcgctcgcg accgctgagc ttgagcgagt gggcgttctg agcgtccttg aactgcagcg
      661 gcagcactcc catgccgatc aggttcgagc gatggatgcg ctcgaagctc tcggcgatca
      721 ccgcccgcac acccagcagc atcgtgcctt tcgccgccca gtcgcgtgaa gagccggtgc
      781 cgtattcctt gccggcgatg atgatcagcg gcgtgttgtc cgccttgtat cgcatcgccg
      841 cgtcgtagat gctcatctgc tcgccgctcg gaagatattc ggtgataccg ccttcggttc
      901 cgggcagcag caggttgcgc aggcggatat tggcgaacgt accgcgcatc atcacctcat
      961 gattgccgcg ccgcgcgccg tattgattga aatccaccgg ctgcacgccc tgttccacca
     1021 gatagcgggc ggcgggactg ttcttcgcga tgttgccggc cggggagatg tgatcggtcg
     1081 tgaccgagtc acccagcagt gccagcaagc gcgcgccctt gatgccggac accaaggccg
     1141 gtgtcatcgt cataccttcg aagtagggcg gattcttcac ataggtggac ttgctgtccc
     1201 aggcgtagat cttgccctgc ggaacgcgga tgccctgcca gcgctcatcg cccgtgaaca
     1261 cgctgccata gctgcggcgg aacatttccg agttgatgtt cttctgcacg tactcggcaa
     1321 cctcggccgg tgccggccag atgtcgcgca tgtagaccgg cttgccgtca gcgccatgcc
     1381 ccagcggatc gttgttgaga tccacatcga gcgagccggc gagcgcgtac gcgaccacca
     1441 gtggagggga agcaaggaaa ttcattttca cttcgggatg cacgcggccg tcgaagttgc
     1501 gattgcccga caacaccgag cagccgatga tgtcgcccgc cttgacgccg gcggagattt
     1561 catccttgag cggaccactg ttgccgatac aggtggtgca gccgtatccg accaggttga
     1621 agccgacgct ggccagctca tccagcaggt tggccttctt cagatagtcg gtgacgacgc
     1681 gagagccggg cgcgaggctg gtcttcaccc acggcttgga attcaggccg cgctggtgcg
     1741 cattgcgagc cagcagtccg gcagcgatca gcacgtacgg attggaggtg ttggtgcagc
     1801 tggtgatcgc ggcgatcagc acagcgccgt ccttgagttc gaacgacttg tccgcgagcg
     1861 tgacctgggc gttgccggtg gcgccgggat tcttcctggc acgctcctcg gccatgcggg
     1921 catgattctt tgcataggtg ctcttggcga cgcgcagcgg cacgcgatcc tgcggccggc
     1981 tcggtccggc gaggctcggc tcgacgctgc cgaggtccag ctgcagtacg tcggtgtatt
     2041 gcgccggctt ggcgccgtcg tgtcgccaca ggccctgcgc gcgcgcatag tcctcgacca
     2101 ggtgtatctg ttccggcgga cggccggaga gcttcagata gcgcagcgtt tcctcgtcga
     2161 tcggaaaaat cgcgcaggtg ctgccgaatt ccggcgacat gttgccgatg gtggcgcggt
     2221 cggccagcgg cagattcgcc aggccgtcac cgaagaactc gacgaacttg tccaccacgc
     2281 ccttcttgcg cagcatttcg gtgaccgtga gcaccagatc ggttgcggtg gtgccggccg
     2341 gcaggctgcc cgtcagattg aaaccgatca cctgcgggat cagcatcgtg accggctgtc
     2401 cgagcatcgc ggcctcggct tcgataccgc cgacgcccca gccgagcacg ccgaggccat
     2461 tgatcatcgt ggtatgcgag tcggtgccga ccacggtatc cggataggca tgacgctcgc
     2521 tgccggtgtg tttgtcgaac acgacccggc ccaggtattc gagattgacc tgatgcacga
     2581 tgccggtgtt cggcggcact actttgaaat tgcggaacgc tgactggccc cagcgcagga
     2641 acgcgtagcg ctcgccgttg cgctcgaact cgatacgatt gttgtcggcc agcgcgtgtg
     2701 ccgtgccgta gctgtccacc tgcaccgagt gatcgatcac cagttcagcc ggtgcgagcg
     2761 gattgatctc gtcggcgttg ccgcccagcc gggtcatcgc ttcgcgcatc gctgccagat
     2821 cgacgatcgc cggcacgccg gtgaagtcct gcatgatcac gcgcgcgggc gtgaatgaaa
     2881 tctcgtacga tggtgtggcc ttggggtccc agttcagcag tgcttcgacg tccttcaccg
     2941 tgacgttggt accgtcctcg aagcgcaata gattctcgag caggatcttg agcgaatagg
     3001 gcagacgcga caccttctcg gcaccgagtg ccggcaggct ccatatatcg tatgtgtgat
     3061 cacctgatgt cagcgtggtg cgggtcttga aactatcgtt catacatcct ccgtcgaggc
     3121 cgcgcgcgaa ttcgagtggc gcgacaaagc ttccgaatta tatcgggcct gctcgatcac
     3181 gccaccgccc agacagcgat ccgcgtcgta cagcaccgcg aattgtcccg gcgttgcggc
     3241 ccgctgcgga tgctcgaatt cgatgagtgc agcaccgtca tcacctgctt cgatgcgggc
     3301 cggttgcgcg ctctggcggt agcgcagctt cacctgcgcg ctgaatgagc cggtgcgcgc
     3361 cgcgcacagc cagttgattc ggccacacac gaccgcgttg gcatcgagca ggtgctgctc
     3421 gtgccgctgc acgacgatca gtgcgttgcg cgtgctgtcc ttgcgggcca cataccaggg
     3481 ctcctgcgcg tagccgcgcg ccccgccgat ttccaggccc gagcgctgac ccagtgtgta
     3541 gaacggcagt ccacggtgtt gtccgagcac acggcccagc ggcgtttcga tcgggccggg
     3601 ggaatcctgc agaaagcggc cgagaaactc ggcgaacggc cgctcgccaa tgaagcagat
     3661 gccggtgctg tcaggtttgt cgtgtaccgg cagacccgat tggcgcgccc gctcgcgcac
     3721 ttcgtccttg tgcaactcag ccagcgggaa cagcacctgc gcgaagcgag cgcgttcgac
     3781 cgcatgcaga aaataggact ggtccttggc gtggtcgact gcccgataca gcgccggccc
     3841 gtccgacagc tgctgcacgc gggcgtagtg accggtggcg tagaactgcg cgcccaggcg
     3901 ctgcgcatag ttcaggcaca ggccaaattt gatctcgcgg ttgcacagca cgtcgggatt
     3961 gggcgtgcgg ccggcctgat actcgtccag aaaatactgg aacacccggt cgcggtattc
     4021 gtcagcgaaa ttgacccggt gcaggggaat cgccagatcg gccgccaccc gccgtgcatc
     4081 ctgatagtcc tgcgcggtcg tacagtagtc atcgtcctgt tcccagttgc tcatgaacag
     4141 tccctgcacg tcgtgaccgg cgtcgtgcag caacagagcg gcgactgcgg aatccacacc
     4201 gccggacatg ccgacaatga cgcgtgcagg cgcgccaggg ttcatggcga gggccgctgt
     4261 tcgggcactc gctggttcag cacctcgttc gcgatcgcag ccagcggcag gcgttgaccg
     4321 gcgaggtagt cgtcgatgca gcgcgtgacc aggacggtgc gaagctgcgc ccgtcgctgc
     4381 aggatgtcag cacgcgtgag ccagtgcgtt gcctggaccg gcggatcgag cgcgcgctcg
     4441 gcatcgaaat cgctcacgct tccggtgaac gcaaagcgca gcgtggtgtg agcgcgctga
     4501 ctgcgccaca gatagatgcc caccagtgcc gaaggcgcaa agctccaggc ggtttcttcg
     4561 agcgtttcgc gcaccacggc ctgtatcagg gattcgccgt cctcgagatg gccggcgggt
     4621 tgattgagca ccaggtggcc gcggatgagc tcttccacca acaggaagcg atcgtcgcgc
     4681 tcgatgatgg cggcgacggt cacgtcgggt ttccatacca tgcgcgaagg ataccctgcg
     4741 cgccagcgcg ctgcagcggc ggtgaactag cggcggtgaa ctagcggcgg gcgattctga
     4801 cttcgcgctc ttccggcgcg ctggccagcc agacggtatc gaattcctgc aggtactggc
     4861 gcgcgacctt cggattgttg aatcctgcca ggccatcggg gcggtcggcc cgtggacggt
     4921 aaacgatcgc gcgatcgtca gcgatgcagt aggcggcggt cagctcgcga tactgcacgg
     4981 cgcggatgcg aatctccaga tagctggtga gccggtgtgc catcgcaacg aagcggttgg
     5041 cgccgttgcc gaggcgtgcc tggtcgcgca tcagcacgcg caccttggcg aaactgtgtc
     5101 cgagcacgaa gcgcttgatc acctcgagca gtgccggctg atcgaagatc tccggttcca
     5161 ggtccggggt catgatcgag atcaggcgct ggccgctggc cgccaccgtc tcggtggccg
     5221 agcgcacctc gtcgaaggtg ctcagcacat gcagcgtttc cggaacgggt gctttctgag
     5281 ccggcctctg ggacagcgcg ttggagacca ccggaccgtc ttcgagcccg aagctcgaca
     5341 gtcctgccga tgtggtcgtg cggcttgccg ttactactgt cactcgcccc gcctgggatt
     5401 attcggccgt ctgacaggga atgttagaga caggcttacc gattgatcgc caaccggttc
     5461 acatttgtgc gcctgcgctg tgtgagcggg ataacgaaat atcccatcag tcgccaatga
     5521 agattatgtt actcgtcttc ggtcccaaaa cggcgcgcca ggtgggccgc caagccgata
     5581 tattgcgatg gtttcagcgc ggccaggcgc tgccggtcac cctccggcag tggcagcgtc
     5641 gcaacaaatg ttcgcagcga gtcggcgtcg atacgctgtc cacgggtgaa ctgcttgagc
     5701 tgttcgtagc cgttcggcac accggcgagc cgcagcacgc tttggatggc ctctcccagg
     5761 gtctcatagg cctcatccag gtcctcggac atacgctgcg gatccgggct gatgcgtgcc
     5821 agtccgcgct gcagagcctt ccatccgatg aaactgtgcg ccatcgcgac gcccagattg
     5881 cgtaacaccg tcgagtcggt gagatcacgc tgccagcgtg agagcggcaa tttgtcggcg
     5941 aagaagcgca gcatggcgtt cgcgagcccg aagttgcctt ccgcgttctc gaagtcgatc
     6001 ggattgacct tgtgcggcat ggtcgaggag ccgacctcgc ccgccagcgc gcgctgtcgc
     6061 agatagccga gcgagatgta gccccatgcg tcgcgcgacc agtcgatgag gatcgagttg
     6121 atcgcggccg tcgcatcaca gaactccgcg atccagtcat gcggctcgat ctgcgtgctg
     6181 taggcattcc attgcaactg ctgctggcga atgaacgttt cgctcaagcc gatccaatct
     6241 atattctcgc gcgcgctgtg gtgcgcgttg tagttgccga ccgcgccgtt ccacttggcg
     6301 agaatcggca cctgctgcaa gcgtcggcgt gcgcgacgca ggcgtgcgcc gacgttggcg
     6361 acttccttgc cgaaggtggt cggactggcc gcctgcccgt gggtgcgcga gagcatcggc
     6421 aggtccgcat actggcgcgc gagctgactc agcgtctgca gcagctgcgt gagctcgggc
     6481 agcagcagct gctcgcgcgc cgcgctcatc aggcgcgcgt agctcagatt gttgatgtct
     6541 tccgaggtgc agccgaaatg caccagttcc agtgcgcgct ggtcggcgcc ggcgttcgcg
     6601 agtacctcgc gcacgaagta ctcgaccgac ttgacgtcgt ggttgatgcg cgtctcgatc
     6661 gccttcacct gcggtgcggc gtgcgcaccg gggtccgcga cgagacctga aacggtttca
     6721 tcgaccgctg ccggcagagg cgagtgcagc agccccagag ccgggtcgct caattgtcgg
     6781 aaccacaagg cctcgacccg gatgcgctcg cggatcagcg cgccttcgct gaagtacgcg
     6841 cgcagcggtt cgctggtgct ggcgtagcgg ccgtcgagcg gcgaaagcgc gttcagtggg
     6901 gacaaatcca tggcgatgcc gcgtatgata acgcattgat agaccgtgcc ggagtgagcg
     6961 cgccaatcat gaactcgacc tcgtttcgaa ccgaacgcga cagcatgggg gaactgcaag
     7021 tcccgtccga cgcgctgtgg ggggcgcaga cgcagcgtgc cgtcaataat ttcccgatca
     7081 gcggactgac tctgccgcgc agcttcatac gggcgctggc gctcatcaaa caggcggccg
     7141 cggcggccaa tgtgcggctc ggactgctgg agcgcgcgct cggtgaggcg attgtgcgct
     7201 gtgccgccga ggtggccacc ggcgcgcacg accggcagtt cccgattgat attttccaga
     7261 ccggctcggg caccagcagc aacatgaacg ccaacgaagt gatcgcgcac ctggcgctgc
     7321 ggtccggcac cccggtgcat gccaacgatc acgtcaatat gtgccagagc agcaacgatg
     7381 tcattccgac cgccatccat gtgagcgcgg cactgatgct gaaggaggag ctgctgccct
     7441 cgctgcagca tctggcgcaa gtgatcgggg cgcgggaggc agagctcatc catatcgtca
     7501 agaccgggcg tacccacctg atggatgcga tgccggtgac gctcggacag gaactgtctg
     7561 cctggcgcac gcagatcgag aacggcacgg cgcgcctgcg cgcggtggaa ccgcgcctgc
     7621 tgtcactggc gcagggcggt accgcggtag gaaccggtat caatgctcat ccggagttct
     7681 cggcgacggt gtgcagcgag ctgcagcgcc tgacggcaat cgcgttcaaa ccgagctcca
     7741 acttcttcga atcgctcgcg gcgcaggata ccgcggtcga gctgtccgga cagctgcggg
     7801 tggtggcagt gagcctgatg aagattgcca acgatctgcg ctggatgaac agcgggccgc
     7861 tggccgggct gggtgagatc gcgctgccgg cgctgcagcc gggcagcagc atcatgccgg
     7921 gcaaggtcaa tccggtgatt gcggaggcca ccgccatggt tgcggcgcag gtgatcggca
     7981 atgatgccac catcgcggtc gccggccagt caggcagttt ccagctcaac gtgatgctgc
     8041 cgctgatcgc ctacaacctg ctcgagagca tccgtctgct gagcaacgtc tcgcgcgcgc
     8101 tcgcggaccg cgcgatccga ggcttcaccg tcaatcgtga gcggctcgcc gaagcggttg
     8161 atcgcaatcc catcctggtg acggccctca atccggtgat cggctatgag aagggcgcgg
     8221 cgatcgccaa gaaggcctac gccgaaggca agccgatccg cgatgtggcg caagcgcaga
     8281 cctccttgtc gcgcaaggag ctcgaccagt tgctcgaccc gttcgaactc acgcgcggcg
     8341 gcatccacgc cggcggtggc ggcgggggct gaaggcgggc ggaggatggc tgaagccgcg
     8401 gcctcgattt cgccccagct cccggcggac tggcgcgcgc gcctcagccg ggggctgctg
     8461 acacagccgg atgtcgagca ggagaatgtg cgcctgggcg gactggcgtc gcattcggat
     8521 agcgtgcgtg cgcgcgtgcg cgaggcctgg ccggagaata tgcgcgccgc ggcggtgctg
     8581 gtcggtatcg tcgatcggcc cgaggccccc acgctgctga tgaccgtgcg ggcgcggcat
     8641 ctgcgtcagc acccaggcca gatcagtttt cccggcgggc gcctcgagcc cttcgatgcc
     8701 gacatcgccg cagcggccct gcgcgagacg catgaggaga tcgggattgc cgtgggcttc
     8761 attcagccgc tcggcttcct gaccgatcac gtggtgcaaa ccggctttcg catcacgccg
     8821 gttgtggcga tgatccgccc gggcttcacg ctcaatcccg acagcaccga ggtcgccgat
     8881 gtcttcgagc taccgctgtc gctggcgctg gcgatcgaga gctatcgggc gcagcagcgc
     8941 atggtgcgtg actttaaggt ggacgtgtgg gagctgccgt tcggcgagta tcacatctgg
     9001 ggggcgaccg cgggaatcct gatgaatctg tgcgaagtcg tcagccgagc gcacgcatga
     9061 gcaaggcggc cgaagagctg gatgcgttgc tgcggctgat gcgcagcttg cgcgcgccgg
     9121 atggctgtcc gtgggaccgc gagcagacct tcgcgaccat cgctcccttc accatcgagg
     9181 aagcgtatga ggtcgcggat gcgattgggc gtggcgatct gacgcgtctg cgcgatgagc
     9241 tcggggatct gctgttccag gtggtatttc atgcgcgcat ggcggaggag gccggaaagt
     9301 tcgactttgc cgatgtcgcg cgcgcgatct gcgacaagct gatccgccgg catccgcaca
     9361 tctttgccga acgcaagcaa ctggatgtcg cgcagctgca cgtttcctgg gaggcgcaga
     9421 aagcgctcga gcgggccgag gcaggccatc acggggtgct gtcggatgtt cctcgttcgc
     9481 tgccagcact ggcgcgtgcc gccaagctgg gtaagcgcgc cggacgggtg ggcttcgact
     9541 gggacagcgc cagcggggtg cgggccaagc tcgacgaaga gctgggtgaa atcgatgagg
     9601 cgctgaaggc cgctcaccct gaagcggtgg ccgatgagat cggcgatctg ctgttcacgg
     9661 tcgcgaactg ggcgcggcac ctgggggtgg atccggagag cgcgctgcag cgagccgcgc
     9721 tgaaattcga aacccggttc gagcgcatgg agcacagcgc cgggggccag aacctgagcc
     9781 tgtcgcagct gtccgcggcc cagtgggagc agctctggca ggcggccaag tccttggccg
     9841 ataagcacta gctgaccgat tcagtcccgg ttcatcgcgg tggccatagc ctttgagaaa
     9901 tggagtcgaa cgctgcggac tcggtcaagc tcgtcaggcg atccgcgtgg gtggccgcag
     9961 tgatcttgtt gctgggcctc gccggatcgt gcgtggcctc ggcggtcgag cccgccaacc
    10021 tgcgcgagat ttcgcgtgaa caggctacac aggagctgca aaagcgctac ggtactgcgg
    10081 cccgggtcat ccgtaccgac gtcatcgagc agggggggca ccacgtgtat gtctttcgcc
    10141 tgctgtcggc caatggcagg gtgtgggtcg tgcgcatcga tgcgcagagc ggggccgaag
    10201 tgccttgagg gagtggctta agtgcgtgtg ctggtagtgg aagacgagtc ggcattgcgt
    10261 gaggcgttga aggttgaact ctcggcagcc ggctacaccg tcgatctggc cgccgacggt
    10321 gaggaaggcc tgtacgcggc caccgagtac ccgatcgatg tggcgatcat cgatctgggt
    10381 ttaccgaaga tgtccggact ggagctgatc cggcagctgc gcgccaaggg taaatcctat
    10441 ggcgtgctga tactcaccgc gcgcgatcgc tggcaggaca aggtcgaagg actcagtgcc
    10501 ggagccgatg actacgttcc caaaccgttt cacttcgccg aggtgcaggc acgactggca
    10561 gcactgctac gccgcgccgg cggctgggcc agtccgacgc tcgcatgcgg gccggtggag
    10621 ctggacacgc gcgcgcagac ggtgagcgtg gatggcaagg cagtggagct gaccagcttt
    10681 gaataccgca ttctggagca tctgatgctg cgcgccgggg aggtgatctc caaaacggag
    10741 ctcaccgagc gcctctatga tcaggacttc gatcgtgaca gcaatgtggt cgaagtgctg
    10801 gtgggacgcc tgcggcgcaa actcgatccg gacgacgcgc tcaagccgat cgaaaccctg
    10861 cgcggccgcg gctatcgtct gagcctgccg cgccgctgcg cttaacgact gcacgcgatc
    10921 ttgaaggggc gatccatcgc atgatgcgct caccgccgtc ggtcagccgc agactgttga
    10981 tcgcggtcgc ggtgccgctg ttgctgttct tcgtgctgac cggttgggtg atcgaccatg
    11041 tgttccagca gcagagcgag gaggcgctac gcgcgcgact cgaagagcag gtggtcgccc
    11101 tggtcaccgc cgtcgacctg gaccgggacg gcaatctgat ggtcaatcag ctcgacccgg
    11161 agcactggct cgaggtgccc ggctccggac agtacgcctc gctgcgcgat gagaagggca
    11221 agctcatctg gaattcaccg tcgctgtcag gcaccgggct ggaactcggc tccaagctgc
    11281 cgcagggttc ggagagcttc cgctcgctgc tggcgcgtga cggtagcagc gtcgcggaac
    11341 tgagccgtcg cctgcatctt gatctgggcg gcgtcaaaga gaacctgctg ttcacggctg
    11401 ctgacagcac cgcgccacag gtgcggcaac tgcggaaatt ccggcagcgt atggccgagt
    11461 ggttcggcgc tctggcgctg gcgctgctgg ccacgatggc gtggatgatg cggcgggcgc
    11521 tcgcaccggt gcgccgtttg gagcaggaaa tcgccgctgt cgaactgggt gcggcgcagt
    11581 cgctgggcga cgcctatccg cgcgagctcg ccggcgtgac cgagggtctg aacgcgctgc
    11641 tcgaatccga gcgcaaccgc atcgcgcgct atcgcgacac gctcggcaat ctggcgcata
    11701 gcctgaaaac accgctcgcg gtcattcgcg ccagcctcga caacgtgcag agcgatgctg
    11761 cgcgcacgca gtccaccatc gagaacgaga tcgagcgcat ggcgcagatc gtcgcccacc
    11821 agctcaagcg cgccgcggcc ggcggcggcg cgacgctcgg ccaggcacca ttgccggtgc
    11881 tgccgctggt gacggacctg cgatcggcaa tgctgaaggt gcacgcgcgc aaggatctgc
    11941 gcatcgacat cgatgtgccg cccgaaccgg gctttctcgg cgacagcggc gacatcctgg
    12001 agctgctcgg caacctggtg gacaacgcct gcaaatggtg tcgttcgcgg gtgatggtga
    12061 gtgcgtcgct cgatccggcc cgcgagctgc cacggcgact gtcgatcgtg gtggaagacg
    12121 acggaccggg tatcgcgccc gagaatcgca cgcgcgtgat cgagcgcggc gtgcgcgcgg
    12181 acgagcacgt gcccggccat ggtctgggac tggcgatggt gcgcgagacc gccgggctgt
    12241 acggtggcag gttcttcatc gacgaatcgg tcgcactggg cggcgcgcgt gtcgaactgc
    12301 agctgccggg gcgctaaccg aactcaatgc cctgcgcgag cggcagggca ttgctccagt
    12361 tgatcgtgtt ggtctgccgg cgcatgtagg ctttccaggc atccgagccg gcttcacgcc
    12421 cgccgccggt gtccttctcg ccaccgaacg caccaccgat ctccgccccc gaggtgccga
    12481 tattgacgtt ggcaataccg caatcgctgc ctcgatgact gagccaggtt tccgcgttgc
    12541 gcaggctctg cgtgaacaga ctcgaggaca gtccgtaggc ggaagtgttc tgcagggcga
    12601 tcgcctgctc cagtgagtcg accgtgatca gatagagaat cggaccgaag gtctcctgct
    12661 gcacgatctc ccagtcgttt tgcgccacga cgatggcagg ctccacatag tgccccgggc
    12721 gttgcagcac gttgccgccg accagcatct cgccaccggc ggcctgcgcg gcggcgactg
    12781 ctcgctcgaa ggcacgcacc gcgttgtcat
//
LOCUS       DATMRM010000292         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2015663, whole genome shotgun sequence.
ACCESSION   DATMRM010000292 DATMRM010000000
VERSION     DATMRM010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2015663"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VNZ06_08570"
     CDS             <1..394
                     /locus_tag="VNZ06_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20843.1"
                     /translation="YAPALAQRGLPLERMLLIRTQTWQQSLWATEQALRCPAIGAVLS
                     WPDYIVDQNVRRLQLAAEAAGTVGFLYRPPAAAQESSPAALRLRLHPASDGIIVEIHK
                     SRGGRAGARLHCRDPHAVAVHPSAAARA"
     gene            354..1628
                     /locus_tag="VNZ06_08575"
     CDS             354..1628
                     /locus_tag="VNZ06_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HWW20844.1"
                     /translation="MLWLCIRLPQLAREALAANTRAAMESTMERLAAWAYQWSSQVSY
                     HLSDPPLLWLELGASHRLFGGYAALMTRLEAGLTQLGYQHACALAPSAHGAALLTQLE
                     RQRCVYTRAQLRLRLEPLPLRLLELPSEITDALHSAGLRSIGQLLRLPATAIARRFGP
                     QTSVYLRRLLGQQSDPRPSWQLPPTYHARCEFGCELRDTTAMLFPLQRLLLEFQGYLR
                     GRDCAVQRFTLRLEHYRHPASELIIGLSAPGREAAQFLQLVRERLHDLVLPAAVSGLA
                     LEAHEFTSPSVVQADFFGSNAQQLQQLQLLLDRLRARLGTDHVQGLQPEADYRPERAW
                     SFAPPATTQHDHNGVAQDQQPARPCFLTTDLQRVAPPARLLSGPERIESGWWDRADAA
                     RDYYIAEGQDGARLWVFRDLSNGGWYLQGLWT"
     gene            1643..>2789
                     /locus_tag="VNZ06_08580"
     CDS             1643..>2789
                     /locus_tag="VNZ06_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWW20845.1"
                     /translation="MRTESAYAELHALSNFSFLRGASHPAELIAQAHALGYRALALTD
                     ECSLAGVVRAYEAQRELKDPDFGLIIGSEFRTVCGLKLVLLAPSQRAYGQICQLITLG
                     RRRSGKGEYRLSRDDFESGLEDCLALWVAARDATAAQARWLHDFFPARGWLAVELHRA
                     ADDAARLDAALNLATRHDLPAVASGDVHMHLPTRRPLQDVLTAIRHGCTVDEAGWRLH
                     PNAERHLRQIRDLRTLYPPSLLDESVAIAERCRFSLSELHYEYPPELVPAGLTASEHL
                     RALTHTGLKRRWPRGAPASVREAIARELALIAELRYEHFFLTVHDVVEYARRENILCQ
                     GRGSAANSAVCYALGITEIDPARMQLLFERFISRERNEPPDIDVDFEH"
BASE COUNT          453 a          935 c          897 g          504 t
ORIGIN      
        1 gtacgcgcca gcactggcgc aacgcggcct gccacttgag cggatgctgc tgattcgcac
       61 ccagacatgg cagcaatcgc tctgggccac cgagcaggcg ctgcgctgcc cggcgatcgg
      121 cgcggtgttg agctggcccg actatatcgt cgaccagaac gtgcgccgcc tgcagctcgc
      181 ggccgaggcc gccggcaccg tgggatttct gtatcgcccg cccgcagcgg cgcaggagtc
      241 ttcacccgcg gcgttgcggt tgcgattgca ccccgcctca gacggcatca tcgtcgaaat
      301 tcataagtcg cgcggcggtc gtgctggcgc aagactgcac tgcagggatc ctcatgctgt
      361 ggctgtgcat ccgtctgccg cagctcgcgc gtgaagcgct cgccgccaat accagggcgg
      421 ccatggaatc gacgatggaa cgcctcgcag cctgggccta tcagtggagc agccaggtca
      481 gctatcactt aagtgatccg ccgctgctgt ggctggaact cggcgcgagt catcgattat
      541 tcggcggcta cgctgcgctg atgacgcggc tggaagcggg cctgacgcag ctgggctacc
      601 agcatgcctg cgctctggcg ccctccgcgc acggcgccgc attgctgacc cagctcgagc
      661 gccaacgctg tgtctacacc cgtgcgcaac tgcgcctgcg gctcgagccg ctgcccttaa
      721 gattgctgga actgccgtcc gagatcaccg acgcgctgca ctcggcggga ctgcgcagca
      781 tcggacagct gctgaggctg cctgccaccg cgatcgcacg tcgctttggt ccgcagacca
      841 gtgtgtacct gcgacggctg ctgggacagc aaagcgatcc acgcccgagc tggcaattgc
      901 cgccgaccta tcacgcgcgt tgtgagttcg gctgcgagct gcgcgatacc actgcgatgc
      961 tgtttccgct gcaacggctg ctgctggaat ttcagggcta cctgcgcggt cgcgactgcg
     1021 cggtgcagcg cttcacgctg cggctcgaac actatcggca tccggcgagc gagctcatca
     1081 ttggcctgtc cgcaccgggg cgtgaagcgg cgcaatttct gcaactggtg cgcgagcggc
     1141 tgcacgatct cgtgttaccg gcagcggtga gcgggctcgc gctcgaggca cacgaattca
     1201 cttccccttc cgtggtgcag gcggatttct tcggcagcaa cgctcagcag ttgcagcagc
     1261 tgcagttgct gctcgaccgt ctgcgtgcgc gcctggggac ggatcacgtt cagggtcttc
     1321 agcctgaagc cgattatcgg cccgaacgcg cctggagctt cgcgccgccg gcgaccacgc
     1381 agcacgatca caacggtgtc gcgcaagatc agcaacccgc gcgaccgtgc ttcctgacaa
     1441 ccgacctgca gcgcgtggcg cccccggcac gactgctgag tggaccggag aggatcgaga
     1501 gcggctggtg ggaccgcgcc gatgcggcac gcgattacta cattgctgaa ggccaggatg
     1561 gtgcgcgcct gtgggtgttc cgcgacctaa gcaacggcgg ctggtatctg cagggtctgt
     1621 ggacttagcc tcaggtgacg gcatgagaac cgaaagtgct tacgccgaac tgcatgcgtt
     1681 gagcaatttc agtttcctgc gcggcgcttc ccatccggcc gagctgatcg cgcaggccca
     1741 tgcgctcggc taccgcgcgc tggcactgac cgatgaatgc tcactggccg gtgtagtgcg
     1801 tgcctacgaa gcacagcgcg aactgaagga tccggacttc gggctgatca tcggctccga
     1861 attccgcacc gtctgcggcc tgaagctggt gctgctcgcg ccctcgcagc gcgcctatgg
     1921 gcagatctgt cagctcatca cactcggccg gcgccgctcc ggcaagggtg agtatcgcct
     1981 cagccgcgat gacttcgagt cgggcctcga ggactgtctt gcgctgtggg tggcagcgcg
     2041 cgatgccacc gccgcacagg cgcgctggct gcatgatttt ttccccgcgc gcggctggct
     2101 ggcggtggaa ctgcaccgcg ccgctgacga tgcggcacgc ctcgatgccg cgctcaatct
     2161 tgcgacccgt catgacctgc cagcggtcgc cagcggtgac gtgcatatgc acctgcccac
     2221 ccgccgtcca ctgcaggatg tgctcaccgc gatccgccac ggctgcacgg tcgatgaggc
     2281 cggctggcgc ctgcatccca acgccgaacg gcatctgcgt cagatcaggg atctgcgcac
     2341 gctctatccg ccgtcgctgc tcgatgagag cgtggcgatc gccgaacgtt gccgcttctc
     2401 actgtccgaa ctgcactatg aatatccacc ggaactggtg ccggcgggac tgaccgccag
     2461 cgaacatctg cgcgcactga cgcacaccgg tctcaaacgc cgctggccgc gcggggcgcc
     2521 ggcctcggta cgtgaagcca tcgcgcgcga actggcgctg atcgccgagc tgcgctacga
     2581 gcattttttt ctcacggtgc acgatgtggt cgagtacgcc cgacgcgaga atatcctgtg
     2641 tcagggccgc ggctcggctg ccaattccgc ggtctgctat gcgctcggta tcaccgagat
     2701 cgatccggcg cgcatgcaac tgctgttcga gcgtttcatc agtcgcgaac gcaacgagcc
     2761 cccggacatc gatgtggatt tcgagcacg
//
LOCUS       DATMRM010000293         8514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_492265, whole genome shotgun sequence.
ACCESSION   DATMRM010000293 DATMRM010000000
VERSION     DATMRM010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492265"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2253)
                     /gene="ligD"
                     /locus_tag="VNZ06_08585"
     CDS             complement(<1..2253)
                     /gene="ligD"
                     /locus_tag="VNZ06_08585"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HWW20846.1"
                     /translation="MKRPLDRYASKRSFAKTPEPGPQLPTTRGGPLLFVVQKHAARRL
                     HYDFRLELDGVLKSWAVPKGPSLMASDKRMAVEVEDHPFDYGSFEGVIPSRQYGAGNV
                     IVWDCGIYSPDEDRQYSFNDRIEAQQRVREGLTAGKLSFFLRGEKLKGSFALVRTRAD
                     KQWLLLKHKDRFADSNDLLARSRSVLSGATLDDISPAVDAARLQAASLVPTGPAESMP
                     PKLSPMLAESGEQPRSSPEWLYEPKLDGYRVIAFVQGTTVRLQSRRGIDLTSAFPEIT
                     QELAQQADSQMVIDGEIVALGSGGRPSFNALQNRVQLKARAEIDNARREAPVVLLCFD
                     LLHFGGINLRNASYTDRRRYLTQCLLPSAHLQLVHAATDAAPLYAAATAMGFEGIVAK
                     RRNSTYQPGKRSNAWVKFKRTQTTEFVVGGYTRGKGEREPLGALLLGAWNDGKLQYVG
                     HVGSGLTVDVIDDIGERAKGLARKRSPFSGKVELHRPTHWLEPKLVVEVTFAERTPDG
                     LLRAPVFMRLRDDVDPEEITAGTSTACAVSGQSSSRAASEQPSSEIDRVVSQLKNTAT
                     KVELSVGGARIRLTNLDRVYWPAQARGQPAITKRDLITYLARVSSYMLPHLKDRPLTM
                     IRMPEGIDGERFFQKHWDQAMPEFVETVELFSDHKDEKHRYILANNLPTLLWLGQNGT
                     LEFHVMHSRASVGPDCINRNTDYSSSLEALKDSVLNYPDYLVFDIDPYIYSGLEPKGA
                     EPELNTRGFAV"
     gene            complement(2250..2915)
                     /locus_tag="VNZ06_08590"
     CDS             complement(2250..2915)
                     /locus_tag="VNZ06_08590"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HWW20847.1"
                     /translation="MSIAVGDAHRVSALSIVPPAARACYVFAHGAGAGMSHPFMHAIA
                     MGLAAVDIATLRFQFPYMERGSRRPDPPALCHAAVRAAVAEASRLTPGRPLIAGGRSF
                     GGRMSSQAQALAALPQVEGLAFLGFPLHPAAKPSAERAQHLFDIRVPMLFLQGDRDEL
                     ATLSLLQSVVARLGDHATLHLIAGADHSYKVAAKTGRKAAQVTAELLQALQQWIGSTI
                     RQS"
     gene            complement(2984..3379)
                     /gene="mscL"
                     /locus_tag="VNZ06_08595"
     CDS             complement(2984..3379)
                     /gene="mscL"
                     /locus_tag="VNZ06_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015379263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HWW20848.1"
                     /translation="MSFASEFKEFAMKGNVVDLAVGVIIGTAFGKIVSSFVADVIMPP
                     IGVLIGGINFGDLALKVGADPKGVPVLLKYGSFLQTIFDFLLIALVIFIALRGINKLK
                     KPPPPPVDAPPPRQELLLEQIRDAIVNKR"
     gene            complement(3409..4173)
                     /locus_tag="VNZ06_08600"
     CDS             complement(3409..4173)
                     /locus_tag="VNZ06_08600"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HWW20849.1"
                     /translation="MHIRACFMGCLTLFPIAAWADNAPPPAPQGVWVGKGQLGFLASQ
                     GNTQSTSANAAIDMGLALDPWEHKLHLDGLYGKSSDVVAAEKYEAGWQSQYTVTPVLY
                     GYGALRYEHDLFSGFQYQASETAGVGYKLFNTADTKLSVQVGVGYRELRPETITKDAD
                     GRIIRTLQATNNGAVGTFGLDYTQALTSTTSLANRLLVEAGSGDTLITDTVALTVKMS
                     TRLALSLGYSLQDNTNPPGGLKKIDSVETINLVFAF"
     gene            complement(4316..4816)
                     /locus_tag="VNZ06_08605"
     CDS             complement(4316..4816)
                     /locus_tag="VNZ06_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3761 domain-containing protein"
                     /protein_id="HWW20850.1"
                     /translation="MIPSRIFQTASLATGMLLVAVAFSQVPAGAPAGTTGQCKDGSYT
                     SAASKRGACSGHKGVETWYASSTSASSGGAAPAAPTASAPPAPAAPASAPAPAAAPSK
                     SSSNMPAATAAPGGGPGLVWVNKTSHVYHCQGDRWYGKTKNGAYMSESAAKAEGDRAD
                     HNKPCS"
     gene            complement(4916..5509)
                     /locus_tag="VNZ06_08610"
     CDS             complement(4916..5509)
                     /locus_tag="VNZ06_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HWW20851.1"
                     /translation="MAIRAVFPTLFYQAPLQKRGARELNRRLLGEIRQLQHDDAAGRQ
                     WSRKNYPGGFTSYGSVNRLHAVSPTFGVLERQLADHVKAFCRKLDLNLPTRKLVMTDC
                     WANVMPTGVVHGLHLHPLSTISGTYYVRIPAQAPGLKFEDPRLDRMMAAPRRRAWELI
                     PVKEGDVVLFESWLRHEVPANTARAQRITISFNFNWF"
     gene            complement(5509..6879)
                     /locus_tag="VNZ06_08615"
     CDS             complement(5509..6879)
                     /locus_tag="VNZ06_08615"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 domain-containing protein"
                     /protein_id="HWW20852.1"
                     /translation="MSRPRRTFGPIAALLCGLLGTPALKAEAIAPQDYAYGSLLQVDG
                     RAALYLVTLGEDVYRHTARTDLSDLRVVNGLNEEVPFAIRRPAQRHAEPAAFDPLPLF
                     PLRGADRAPSEALKLRLRASGASIELDQPSGKEGSSVTAYLLDVRGSGVPVTALRLTW
                     IEGTADFSNRLRVEASDDLTRWQLVEQNLPIVNLHYAGQSFVRTEVRLPGQRSSFLRL
                     SWMDAAAPGVILTAVSGQRQVSPVELPRQIVAIAGVASAPAGEYEFDLGAHVPVDRVN
                     VRLPETNTVVEMQFLVRLDDSNWTPIGSGRLYRLQVPGTDELTNSPLSVTLSSQRYWR
                     VRITSAGGGLGSGTPTLEAGWLPGELLFLARGPPPFQLLYGNAAASVPAPGRLGLDAG
                     SLRTPDGPIEAHPAALGAARALGGEARLKAGAPPIDRQRWLLWSVLVAGVGALGTMAW
                     RLARSL"
     gene            complement(6876..>8514)
                     /locus_tag="VNZ06_08620"
     CDS             complement(6876..>8514)
                     /locus_tag="VNZ06_08620"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HWW20853.1"
                     /translation="ALVFGAPTVTMGLQAGMLHNVPYALACTALSLSAFYLLLAWMLT
                     RARRAPVRLLVESFIALSLAFATLAIPLALEDRWTGAAWALEGAAVLWIGLRQERRLA
                     VLAGIVLQLAAAVAYFDDAPVRHREHTRWIIANEHFISEWLLSLGAMISAARVRLKVP
                     AWFTQLQPLVGGGLFLLGLAWWLHAGATQIMRDDVPSHWTHDWLALSAGSAIALSVLG
                     ARLLWNAPRRAALALFPVLAVFAFEASHPHPLAYGGWWIWPLAFAAAYWTLHRDESSA
                     NRGIRTWLHALGLWLLSAVGADELGWQMSRWLPDGPAWSQGAWGLIPAAVLLSAAMSG
                     RTQRWPFASERTAYLQLGPVGLGLFLTVWGAVFDLLADGSAAPLPYVPLLNPIDLAQA
                     TAIVALGLAAAAAARQRGFDRRDDLRSAVRVVIGLQAFAWLNAMMLRFLHHYTGMAYD
                     LNAVVNSTLAQTCLTILWTLIALALMVWANRGARRVGWIAGAALLGVVILKLFTVDLS
                     RTGTLPRIVSFLGVGALMLVIGFFSPLPPRATHEESQ"
BASE COUNT         1618 a         2781 c         2599 g         1516 t
ORIGIN      
        1 gaccgcaaaa ccgcgggtat tgagctccgg ttcggcgccc ttgggttcga gcccggaata
       61 gatgtaggga tcgatgtcga ataccaggta gtccgggtag ttgaggaccg agtccttcag
      121 cgcctccagc gaactcgaat agtcggtgtt gcggttgatg cagtcggggc ccacgctggc
      181 gcgcgagtgc atcacatgaa attccagtgt cccgttctgc cccagccaca gcagtgtcgg
      241 caggttgtta gcgagaatgt agcgatgttt ttcgtccttg tgatcggaga acagctcgac
      301 cgtctcgacg aactccggca tcgcctgatc ccaatgcttc tgaaagaagc gctcgccgtc
      361 gatgccctcg ggcatacgga tcattgtcag cggacggtcc ttgaggtgag gcagcatgta
      421 agacgagacg cgcgccaggt aagtgatcag atcgcgcttg gtgatggcag gctggccgcg
      481 tgcctgggcc ggccagtaca cgcgatccag attggtgagt cgaatgcgtg cgccgccgac
      541 cgacagttcg actttcgtcg ccgtgttttt caactgtgac acaacgcggt cgatctcact
      601 gctcggctgc tcgctcgcgg cgcgggatga gctctggccc gatacggcgc aagccgtgga
      661 ggtgccggcc gtgatctctt caggatcgac gtcatcgcgc agtcgcatga acaccggtgc
      721 tcgcagcaag ccatccgggg tgcgttcagc aaacgtcacc tcaacgacca gtttcggctc
      781 gagccagtga gtggggcgat gcagttcaac tttgccgctg aacggtgagc gcttgcgcgc
      841 aagccctttg gcgcgctcgc cgatgtcgtc gatgacatcg accgtcaggc ccgaaccgac
      901 atgacccacg tactgcaact tgccgtcatt ccaggctccc agcagcagtg cgcccagcgg
      961 ctcgcgctcc cctttgccgc gggtatagcc gccaacgacg aattcggtgg tctgcgttcg
     1021 cttgaatttc acccaggcat tcgaacgctt tcccggttga taggtgctgt tgcgacgctt
     1081 ggcgacgatg ccctcgaaac ccatcgcggt cgccgcggcg tatagcggtg cggcatcggt
     1141 tgcggcatga acgagctgca ggtgtgcgga tggcagcaaa cactgtgtga ggtagcgccg
     1201 tcggtcggtg tagctggcgt tgcgcagatt gatgccgccg aagtgcagca ggtcgaagca
     1261 cagcagcacg accggcgctt cgcgtcgcgc attgtcgatt tccgctcgcg ccttcagctg
     1321 aacccgattc tgcagcgcat tgaacgacgg ccgcccgccg cttcccaacg cgacgatctc
     1381 gccgtcgatc accatctgac tgtccgcctg ttgcgccagc tcctgcgtga tctcgggaaa
     1441 cgcgctcgtg aggtcgatgc cgcgtcgtga ttgcaggcgc acagttgtgc cctgcacgaa
     1501 cgcgatcacg cggtagccat cgagctttgg ctcatacagc cactcaggac tggagcgcgg
     1561 ttgctcgccg gattcggcga gcattgggct gagcttgggc ggcattgatt ctgcgggtcc
     1621 cgttggtacc aggcttgcgg cctgcagacg cgccgcgtcg acggccggcg agatgtcatc
     1681 gagcgtcgca ccggacagca ccgaacggct gcgcgccagc aggtcgttgc tgtcggcgaa
     1741 gcggtccttg tgtttgagga gaagccattg cttgtcagcc ctggtgcgca ccagggcaaa
     1801 cgagcccttc agtttctcgc cgcgcagaaa gaagctgagt ttgcctgcgg taaggccctc
     1861 acgtactcgt tgctgcgctt cgatgcgatc gttgaaactg tactgccggt cttcgtccgg
     1921 ggagtagata ccgcagtccc agacaatcac gtttcctgcg ccgtactgtc tggaaggaat
     1981 cacaccttca aatgagccgt agtcaaaggg atgatcttcg acctcgaccg ccatgcgttt
     2041 atcgctcgcc atcagcgaag gtcccttggg aaccgcccag gatttcagca cgccgtcgag
     2101 ctcgaggcgg aagtcatagt gcagccgccg ggccgcatgt ttctgcacca cgaacagcaa
     2161 cgggccgccg cgagtcgtcg gcagctgcgg cccgggctcc ggggtcttgg caaaactgcg
     2221 cttggacgcg tagcgatcga ggggccgttt catgactgtc ggatagtcga tccgatccac
     2281 tgctgcaggg cctgtagcag ctcggcggtg acttgtgctg cctttcgacc ggtctttgcg
     2341 gctactttat aggagtggtc agcgcccgca atgagatgca gcgtcgcgtg gtcacccaga
     2401 cgcgcaacca cgctctgcag cagactcagc gtcgcgagct catcgcgatc gccctgcaga
     2461 aacagcatcg gcactcgaat atcgaacaga tgctgcgcgc gctcggccga gggtttggca
     2521 gccggatgca acgggaaccc caggaacgcg agaccttcga cctgcggcag cgcagccagg
     2581 gcctgcgcct gcgaactcat acggccaccg aaggatcgac cgcccgcgat cagtggccgt
     2641 ccgggagtca gccgactcgc ttcggcaacg gccgcgcgca cggcggcatg gcacagcgcc
     2701 ggcggatccg gtcggcgcga accgcgttcc atataaggaa attgaaaacg cagcgtcgcg
     2761 atgtctactg ccgccagacc cattgcgatc gcatgcatga agggatggct catgccggcg
     2821 cccgcaccgt gtgcaaacac atagcaggca cgcgccgcag gcggcacgat gctcagcgct
     2881 gaaacccgat gcgcgtcacc gacggcaatg ctcaagtgtt gcgcatcagc gttcacggtg
     2941 tgaagcgaga gggcggcgtg caggccgccg tcccgcggac ccttcagcgc ttgttgacaa
     3001 tcgcgtcgcg gatctgctcc aacagcagtt cctgccgagg cggcggcgcg tcgaccggtg
     3061 gcggtggtgg cttcttcagc ttattgatgc cgcgcaatgc aatgaagatc accagggcaa
     3121 tgagcagaaa atcgaagatc gtctgcagga acgagccgta tttgagtagt accggcactc
     3181 ccttcggatc ggcaccgacc ttcagcgcta aatcaccgaa gttgatgccg ccgatcagca
     3241 ctccaatcgg aggcatgatc acgtctgcga cgaatgagga gacgatcttg ccgaacgcgg
     3301 tgccgatgat cacaccgacg gccagatcga ccacgttgcc tttcatggca aattctttga
     3361 actcactggc gaaactcacg acttgttctc cttgtttccc ctaaggtctt agaaagcgaa
     3421 caccagattg atggtctcca cggaatcgat cttcttcaaa cccccgggcg gattggtgtt
     3481 gtcctgcagg ctgtaaccca agctcaaggc caggcgggtg ctcatcttga ccgtcagcgc
     3541 gacggtatcc gtgatgagtg tgtctccaga gccggcttct accaatagtc tgttcgccag
     3601 ggatgtcgta ctggtgagcg cctgcgtgta gtccaggccg aacgtcccca ctgccccgtt
     3661 gttggttgcc tgcagcgttc gaatgatcct gccgtcggcg tctttagtga tcgtctccgg
     3721 gcgcagttcc cggtagccca cgcccacctg aacgctcagc ttggtatcgg cggtattgaa
     3781 tagcttgtag cccacgcccg cggtctcact cgcctgatac tggaaaccac tgaacagatc
     3841 gtgttcatag cgcaaggcgc cgtagccata aagaacaggc gtcaccgtat attgcgactg
     3901 ccagcctgct tcatattttt cggccgcaac cacatccgag ctcttgccgt acaagccatc
     3961 cagatgcagt ttgtgttccc agggatcgag cgcgagaccc atgtcgatcg ccgcattggc
     4021 ggaagtggac tgcgtgttgc cctgcgacgc cagaaatccc aattggccct tgccgaccca
     4081 gacaccttgc ggcgcagggg gcggcgcgtt gtccgcccag gccgcgatcg ggaacagtgt
     4141 caggcacccc atgaagcatg cgcgaatgtg catagcggtg gttccttgtg gtgagggttg
     4201 aatccaggtc tcgaacgtcg tcgcggtgcg aaaccgcgtc tgggagtcgc tcgctcgaga
     4261 cagaggtcgg gcgggaaccc ccaaggaccc gcaccgaccc gcatcggcta agaacttatg
     4321 aacagggttt gttgtggtct gcgcggtcac cttccgcctt ggcagcggat tcggacatgt
     4381 acgctccatt cttggtcttg ccgtaccagc ggtcaccctg gcagtgatag acatgactgg
     4441 tcttgttgac ccacaccagc cccggtccgc cgccaggcgc ggcggtcgct gccggcatat
     4501 tactgctgct ctttgagggt gccgccgccg gagcgggcgc gctggccggg gccgccgggg
     4561 cgggaggcgc gctcgccgta ggggctgccg gggccgcccc acccgatgat gcactggttg
     4621 agctcgcgta ccaggtctcg actcccttgt gtccggagca ggcgccccgt tttgatgcgg
     4681 cggacgtgta gctcccgtcc ttgcactgtc cggtcgtgcc cgcaggggcg cccgctggca
     4741 cctgcgaaaa agccaccgca accagcagca ttcccgttgc cagcgaagcc gtctgaaata
     4801 ttctgcttgg aatcatggtt ctttcatctc cccttaaaat acgtccccag caaatccgtg
     4861 cctccttgcg gcggcatggc ccacaaggat accagtcggc cgacataacg cccccctaga
     4921 accagttgaa gttgaagctg atagtgatgc gttgcgcgcg tgccgtattc gccggcacct
     4981 cgtggcgcag ccaactctcg aacagcacca cgtctccttc cttgaccggg atcagttccc
     5041 aggcacgacg ccgcggtgcc gccatcatcc gatccaatcg gggatcctcg aatttcagcc
     5101 ccggggcttg cgcggggatc cgcacgtaat aggtaccgct gatcgtggac agcggatgca
     5161 agtgcagccc gtgcaccacg cccgttggca tgacgttcgc ccagcagtcg gtcatcacga
     5221 gcttacgcgt cggcagattc aggtcgagct tgcggcaaaa cgccttcacg tggtcggcca
     5281 gctggcgttc caacacaccg aaagttggcg acaccgcatg cagccgatta accgagccgt
     5341 acgatgtgaa gcctccggga tagttcttgc gcgaccactg cctgccggca gcatcgtcgt
     5401 gttgcagctg acgaatttcg cccagcagtc ggcgattgag ttcacgcgcg ccgcgcttct
     5461 gcagcggcgc ctgatagaaa agcgtgggaa acacagcacg aattgccatc acaagctgcg
     5521 cgccagtctc caggccattg tgcccagcgc acccacgccc gcgaccagca cgctccacag
     5581 aagccagcgc tgacggtcga tcggcggcgc gccggccttc agtcgcgcct cccctcccag
     5641 cgctctggct gcgccgagcg cagccggatg cgcttcgatc gggccgtcag gcgttcgcag
     5701 cgatccggca tcgagtccga ggcgacctgg agcgggcacc gaagccgccg cgttgccgta
     5761 gagcagttga aacggaggcg gtccacgcgc caaaaacaag agctcgccgg gaagccagcc
     5821 ggcttccagc gtcggagttc cggagccgag gccgcccccg gcgctcgtga tgcgcacccg
     5881 ccagtaacgt tgcgagctga gcgtgacgct cagcggcgaa ttggtgagtt catccgtgcc
     5941 cgggacttgc aatcggtaca ggcgcccact gccgatcggt gtccagttcg aatcgtccag
     6001 gcgcaccaga aactgcatct cgaccacggt attcgtctcc ggcagccgga cgttcacccg
     6061 atccaccggg acatgggccc cgagatcgaa ctcgtactcg cctgccggtg cggaggccac
     6121 ccctgctatg gcgacaatct gtcgcggcag ctcgaccggg ctgacttgtc gctggcccga
     6181 aaccgctgtg agaatcaccc ccggtgccgc cgcatccatc caggacagcc gcaggaatga
     6241 agacctctgc cccggcaacc tgacctcggt acgcacgaac gactgcccgg cgtagtgcag
     6301 gttgacgatt ggcaggtttt gctcgacgag ctgccagcgc gtcaggtcat cgctggcttc
     6361 cactctcagt cgattggaga aatccgcagt tccctcgatc cacgtgagtc gcagcgcggt
     6421 caccggcacg ccagatccgc gaacatccag cagataggct gtgacgctcg agccctcctt
     6481 gcccgagggc tgatccaatt cgatcgacgc gcccgaggcg cgcaatcgca acttcagtgc
     6541 ctcgctcggt gcgcgatcgg ctcctcgcag tggaaacagc ggcagcgggt caaatgccgc
     6601 gggttcggcg tgccgctgcg ccgggcgccg gatcgcaaaa ggaacctctt cattgaggcc
     6661 attgaccacc cgcaggtcgc tcagatcagt gcgcgcggtg tgccgataga catcctcacc
     6721 cagtgtcacg aggtaaagcg ccgcgcggcc gtcgacctgc agcagtgagc cgtaggcata
     6781 gtcctgcggc gcaatcgcct ccgccttcaa cgccggcgtg cccagcagac cgcacagcag
     6841 tgccgcgatg ggtccgaagg tgcgtcgcgg ccggctcact gactctcctc gtgcgttgcg
     6901 cgcggaggta gcggcgagaa aaagccaatc acgagcatca gcgcgccgac acctagaaac
     6961 gacacgatac gcggcagcgt tcccgtgcgc gacaaatcca ccgtgaacag cttcagaatc
     7021 acgactccga gcaacgccgc cccagcgatc cagccgacgc gacgcgcacc acgatttgcc
     7081 cacaccatca gcgcgagcgc gatcagagtc cagagaatcg tcagacacgt ctgggcgagc
     7141 gtggagttca caacagcatt cagatcgtat gccataccgg tgtagtgatg caggaatcgc
     7201 agcatcatcg cgttcagcca cgcgaacgcc tgcagaccaa tcaccacacg aacagcgctg
     7261 cgcaaatcat cgcggcgatc gaaaccgcgc tggcgggcgg cggccgccgc cgccagaccc
     7321 aaggcgacga tcgctgttgc ctgcgccagg tcgattggat tgagcaacgg cacgtacggg
     7381 agcggggccg cagaaccgtc tgcaagcagg tcgaacaccg ctccccagac cgtcaggaac
     7441 aacccgagcc cgaccggacc gagttgcagg tacgcggtgc gctcgctcgc gaacggccat
     7501 cgctgcgtgc gcccagacat cgcggcgctc aacagtacgg cagccggtat caatccccag
     7561 gcgccctgag accaggccgg cccatcgggt aaccaacggc tcatctgcca cccgagctca
     7621 tccgctccca cggcgctcag cagccacagt cccaacgcat gcagccaggt tcgaatgccg
     7681 cggttcgcac tcgactcgtc gcggtgcagc gtccagtacg ctgccgcgaa agccaacggc
     7741 cagatccacc atcctccata tgcgagcggg tgcggatgcg aggcctcgaa tgcaaacacg
     7801 gctagcacgg gaaacagcgc cagtgcggcg cgcctgggtg cattccagag caggcgcgca
     7861 ccgagcacac tcagtgcgat cgcgctgccg gcgctcagcg cgagccagtc gtgcgtccaa
     7921 tgggatggta catcgtcgcg catgatctgc gtagcgcccg catgcagcca ccaggccagt
     7981 cccaacagga acagcccgcc gcccaccagc ggctgcagct gcgtgaacca tgccggcact
     8041 ttcaaccgta cccgcgccgc actgatcatt gcgccgaggc tcagcagcca ttccgagatg
     8101 aagtgttcgt tcgcaatgat ccagcgcgtg tgctcgcggt gccgaaccgg cgcgtcatcg
     8161 aagtacgcca cggcagcggc gagctgcagc acaattccgg ccaataccgc cagacgtcgc
     8221 tcctgccgta gtcctatcca aagtacagcg gccccctcca gtgcccaggc cgctccggtc
     8281 cagcgatcct ccaatgccag cgggattgcc agcgtcgcga acgccagcga aagagcgatg
     8341 aacgattcca ccagcagcct caccggcgcg cgccgggctc gagtgagcat ccaggctaac
     8401 aacagataga aggccgacag gctcagtgcg gtgcaagcca gcgcgtacgg cacgttgtgc
     8461 agcattcccg cctgcagtcc catcgtgacg gtcggcgctc caaacaccag tgca
//
LOCUS       DATMRM010000294        15726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2018535, whole genome shotgun sequence.
ACCESSION   DATMRM010000294 DATMRM010000000
VERSION     DATMRM010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15726
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018535"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VNZ06_08625"
     CDS             <1..396
                     /locus_tag="VNZ06_08625"
                     /inference="COORDINATES: protein motif:HMM:NF025715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HWW20854.1"
                     /translation="LALRFDDRLETVERVDRGLIQWGSSWFHTRYHSLHVRSCPVDQT
                     NSSPTSLQQSLTRLHAQLGAAPRMDETSRRLLREVLRDIERVLQDQQPTVRGPQSRLE
                     ELAVRFDVGHPALSASIREIVDLLGRAGL"
     gene            complement(442..933)
                     /locus_tag="VNZ06_08630"
     CDS             complement(442..933)
                     /locus_tag="VNZ06_08630"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HWW20855.1"
                     /translation="MKNRVTWLMVALALTGVAGGVYAQTTPSSAGTAPSDSSTGGNHQ
                     HWHPHRGGMMGGMMMHTLHQLNLTDAQKQSVHSILLTARNNAKAQHQSAGAPNFAALA
                     NPGDPNHGAALQDLLARMTARIQARDAVQQQIYGVLTQDQKTQLASIIAAKQAKWAQH
                     GAS"
     regulatory      1376..1485
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            1609..4131
                     /locus_tag="VNZ06_08635"
     CDS             1609..4131
                     /locus_tag="VNZ06_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWW20856.1"
                     /translation="MRTRQRANLSKAGNLTATPARGWLRRREPVTALTAAVLTALYGA
                     RALAAAGDAGDDNVTPTAAPTSLDEVIVTASRRAVQAEDLPISITAVTGDSLQQAGIQ
                     DIADLAHSIAGISYADKGPFSGVNGSTLIIRGLNSEATAGQLALATPVVPPVATYLDD
                     TPLFFSLRLQDLDHVEVLRGPQGTLYGSGSLGGTIRFVQNAPDLSGFDARAEVTGSDT
                     ARTHEPNGDVNGMLNFALSDALAVRLNAGLSYDAGFINQPDLYVLNSKAQPISADPTN
                     LLSPPLTYSRAGTNEYEYKNGRLAVLWKPNDDFHAQLSYFYQLSTADGFPYDSVLFGL
                     NNLSSSDHTQAHTNDQVNLVSLTLEENFGFATLTSNTSWSHHVNRTDSDLTDLYADFP
                     FYPSLYGSNPRVLVTGHDKLDDKPITEEIRLASATGGFFDWVGGLFYKHETTDIQEHE
                     FYPGYFDYFNACVPVYGVSNGDGVTPSKCGIGETVFTPGTNTFVDGIPIVEDQAYIGD
                     FETKYTDLAAFGELTAHLTSAWSVTGGARVFRQTVEQAQQTGLLFDGPISIDNNSLSN
                     AWNKALWKLNSSYQLDKTNLIYATWSQGFRRGGVNALPPQEPAVNYVTNPGLYKVSPD
                     TADNYEIGVKGTIANRFRYSADIFEIQWHHVQEGAQLTPLVLPASVNLGDAYSQGFES
                     ELYWAPDDHFTVQADYTYDHTRVSTITALAIGGLAVPPPLPGGPLPGTPKNSAALTLG
                     YGHLDAGPGAFRAELTAHYQSSLLPAVSSTIPTVPGYTTLEARLSYQMPHWELTLFGT
                     NLTNVLGINSFSDPTNYQQFYQALITPPRTIGLTLAYSFREK"
     gene            complement(4232..6061)
                     /gene="typA"
                     /locus_tag="VNZ06_08640"
     CDS             complement(4232..6061)
                     /gene="typA"
                     /locus_tag="VNZ06_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HWW20857.1"
                     /translation="MQRPIRNIAIIAHVDHGKTTLVDCLLKQSGTFAAHEKLGERIMD
                     SNDIERERGITILAKNCAIEYGGTRINIVDTPGHADFGGEVERVLSMVDGVLLVVDAV
                     EGPMPQTRFVTRKALALGLKAIVVVNKIDRPGARAGWVVDQTFELFDKLGATDEQLDF
                     PVIYGSALQGWASTDVTRRDPDMTALFEAILQYVPPPPTQHDAALQLQVSTLDYNSYV
                     GRIGIGRIRRGTLKAGKPVALRYGDEDRGTAKINQILTFTGLQRSPVEEACAGDIVAI
                     TGIEDVTIGLTICDPLQAEGLPPIQVDEPTLAMNFQVNTSPLAGREGKFVTSRQIRER
                     LYRELQSNVALRVEDTADADVFRVLGRGELHLTILIENMRREGYEVAVGKPQVLKKVV
                     DGEVHEPFEALTIDIDEDHQGAVMEALGTRRAELTDMVIDGSGRARLDYRVPARGLIG
                     FQGDFMTMTRGTGLMSHIFDGYAVVKGDIPDRHNGVLVSNEDGEAVAYALFALQERGR
                     MFVSPGEKLYEGMIIGIHSRDNDLVVNPIKTKKLTNIRAAGKDDAILLTPPIQLTLEY
                     AVEFIAEDELVEVTPHNIRIRKRFLKEHERKRAAREDATTTAA"
     gene            6169..7122
                     /locus_tag="VNZ06_08645"
     CDS             6169..7122
                     /locus_tag="VNZ06_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWW20858.1"
                     /translation="MFRGSVASQDIEEVVRALKSAAEPSRLRLLCLLSAAEYTVGELC
                     TILGQSQPRISRHLRLLTEAGLLDRFREQQCVYYRMPVAGRYSHWVRQVLSMADPDAN
                     VLKRDRERALRVVGDRARVAQQQVEADELAPREDLASVLREVLGPTSVGELLDIGTGH
                     GLMLEILGPMARHAVGVDICAPALRLARTRVHGAGLAHVEFRRGDMYRLPFDDASFDT
                     VTIHRVLASAERPGAAIGEVARILRPDGRLLVVEDFDLIDARGGSNPLSALRRWFDAA
                     GMSIGHLRPCDLGGRHFIIAFGRHASSASQLSQPQQQAGLA"
     gene            7119..8048
                     /gene="metF"
                     /locus_tag="VNZ06_08650"
     CDS             7119..8048
                     /gene="metF"
                     /locus_tag="VNZ06_08650"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HWW20859.1"
                     /translation="MNGSALSDQMGSDLSALRRPVQISFEFFPPADEKMEQTLWESIQ
                     RLAPLRPRFVSVTYGADGSTRERTHRAVMRVLNETSLLPAPHLTCVGADREHVLDIAR
                     TYWKAGIRHIVALRGDAPKGMSVYQPHPGGFAYAADLVRGLKTVGDFEISVAAYPEVH
                     PEAPDAPFDLQNLKAKLDAGADRAITQFFFDPEVFLRFRDRCVAAGIRASLVPGILPI
                     TRFPQMLKIASRCGTAVPRWLHQRFEGLEEDADTRKLIGAAVAIAQVEALSREGIGEF
                     HFYTLNRADLSYAICHALGVRPTRTASLTEESA"
     gene            8045..11764
                     /gene="metH"
                     /locus_tag="VNZ06_08655"
     CDS             8045..11764
                     /gene="metH"
                     /locus_tag="VNZ06_08655"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWW20860.1"
                     /translation="MNREQRIREFRRLLTERVLLLDGAMGTMIQGYKPDEAAYRGQRF
                     HDWQCDLKGNNDLLTLTQPAMIREIHAKYLEAGSDIIETNTFSSSAPAQGDYKMEGLV
                     SELNLTAARLAREVADSYAERTGRARFVAGALGPTNRTASLSPNVNDPGYRNITFDQL
                     VATYAEATSALIEGGVDLILVETIFDTLNAKAALYAVRRTMRERGVDLPIMISGTITD
                     ASGRTLSGQTVEAFWNSVRHARPIAVGLNCALGAKQLRPYVEEFSRIADIAICAYPNA
                     GLPNAFGEYDEQACETAELVGEFAAAGLVNIVGGCCGTTADHIAHIGEAVGSLAIRVP
                     PKLQPRCRLAGLEALDIGPESLFVNVGERTNVTGSAKFRKLIEADDYNAALDVARAQV
                     NSGAQIIDINMDEGMLDSEAAMVRFLNLIASEPDIARVPVMIDSSKWSVIEAGLKCLQ
                     GKGIVNSISLKEGEAQFLERAGQVRDYGAAVVVMAFDEKGQADTVERKVSICERSYNL
                     LTQKLDFPPEDIIFDPNIFAVATGIEEHNRYALDFIEATAQIRERCPHAMISGGVSNV
                     SFSFRGNEPVREAMHSVFLYHTIRAGMTMGIVNAGQLAIYEQIEPELRERVEDVILNR
                     RADATERLLEIAQRYKGESGTRRAEDLAWRNWPVTKRLEHALVRGIDEYVIEDTEEAR
                     SQFDRPLQVIEGPLMDGMNVVGDLFGAGKMFLPQVVKSARVMKKAVAHLIPYIEKEKA
                     SRGVALGTNGRIVMATVKGDVHDIGKNIVGVVLQCNNFEVIDLGVMVSCEKILEAARR
                     ERADMIGLSGLITPSLDEMVHVAKEMQRQGFTQPLLIGGATTSPAHTSVRIDPQYQGP
                     VVYVKDASRSVGVCQQLIAAGTRETFTARVKSEHALRRSQHGARRTKGPQLTLAQARA
                     NKVREDWQSYAPPVPRMLGLRSFENYSIEELSRYIDWTPFFQAWELRGRFPDILTDAQ
                     FGEQASSLYADARKMLRTVIREQWLQARAVIGLFPANAIDEDLELYEDETRGRVIARL
                     HFLRQQKDLPEGKPHGALADFVAPKSSGVADYIGAFALTAGIGIEPHLQRFEAAHDDY
                     SSIMLKVLADRLAEAFAERLHERVRREFWGYAPDEKLGNEQLIREEYRGIRPAPGYPA
                     CPDHTEKATLWKLLEVDKRAAITLTDSYAMYPTAAVSGWYFSHPQSRYLGVGRIDRDQ
                     VEDYARRKGMSIEDVERWLSPNLGYEPEPSALLIA"
     gene            11761..13632
                     /locus_tag="VNZ06_08660"
     CDS             11761..13632
                     /locus_tag="VNZ06_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWW20861.1"
                     /translation="MNRHEAHLAAPLSAAGGRKSAAGEAEVTRSFFGVFRYSRRAIAL
                     VWDTNRLLLFALAILTLIAGLLPASIAWVGAQIVDAVVTARNAADHNAMRVLRLVLLE
                     GVLVASVAGAQRGLSLAQALLRAQLGQRVNVMILEKALTLELRHFEDSEFYDKLTRAR
                     REASSRPLSLVIRTFGLGQNAISLLSYAALLIRFSPWTMVVLLLAGLPAFIAEAKFSG
                     DAFRLFRWRSPETRMQIYLETVLAREDHVKEVKLFGLGARLLQRYRDIFTRLYGEDRA
                     LSIRRDSWGFGLGLLATTALYGAYAWIALSTIRSLITLGQMTMYLLLFRQGQSAVAAI
                     LSSVGGMYEDNLYLSTLYEYLETPVALSSGHTARGPLPSDGVRFEQVSFTYPGASEPA
                     LVDVTLHLIPGSSVALVGENGSGKTTLIKLLTRLYSPDSGRILLDGLPLPQWDQDALR
                     ARIGVIFQDFTRYQMLVGENVGAGDERYFEDEQRWRDAAGKGQAADFIERLPGRYQTQ
                     LGKWFQNGQELSGGQWQKIALARAFMRTEADILVLDEPTAAMDARAEAQIFEHFRTLA
                     RGHITILISHRFSTVRMADQIAVLDRGRIIEKGSHEQLMALGGHYAQLFSLQARGYR"
     gene            13674..13991
                     /locus_tag="VNZ06_08665"
     CDS             13674..13991
                     /locus_tag="VNZ06_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20862.1"
                     /translation="MKLSTMEETTADTFDSAFSKAVDLGNRLAGKDKEADLWDIADGL
                     LAGAIQYWLYSRQPCGDPRCEDCVPISTADARMAELKRLIDQLAAESEYFHAPTDANA
                     GRA"
     gene            complement(14010..15311)
                     /gene="gabT"
                     /locus_tag="VNZ06_08670"
     CDS             complement(14010..15311)
                     /gene="gabT"
                     /locus_tag="VNZ06_08670"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HWW20863.1"
                     /translation="MSDNSELHRRRGAAVARGVSNAHAIYIERARNAEIWDVEGRQFI
                     DFASGIAVLNSGHLHPRVVTAIEQQLQRFSHTCFQIIPYEGYVALAERLNAITPGPTA
                     KKSLLFSTGAEAVENALKIARYYTRRSALIAFSAGFHGRTFAALTLTGKVQPYKAGFG
                     PLLPDVFHVPFPMAYHAVTPQDCLAAIEQLFKSEVDPERVAAVIIEPVLGEGGFYIAP
                     AEFLQRLRALCDRHGILLIADEIQSGFARTGRMFAFEYSGVEPDLMVVAKSIAGGLPL
                     SAVIGKAAIMDAPPPGGLGGTFAGSPVACAAALAVLDVIRDEQLLARAQQLGSALIAG
                     LRKLQARYHHIGDVRGVGSMVAMELVRNGSAAEPDADLTRALVQAAARQGLIILSCGA
                     YGNVIRFLAPLTISDALLTEGLSRLEAALAEVTRLPATAEA"
     gene            15429..>15726
                     /locus_tag="VNZ06_08675"
     CDS             15429..>15726
                     /locus_tag="VNZ06_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWW20864.1"
                     /translation="MSEQPITTEVVIIGAGPAGLFQVFELGLLGIKCHIIDTLKAPGG
                     QCTELYPDKPIYDIPAIPMCGAQELVDRLMQQIKPFGAQFHLGEETVELKKQPSG"
BASE COUNT         2885 a         4804 c         5018 g         3019 t
ORIGIN      
        1 ctcgcgctgc ggttcgatga ccggctcgag accgtagagc gcgtcgatcg cggcctcatc
       61 cagtggggga gttcgtggtt tcatacccgg tatcactcgc ttcacgtaag atcatgcccg
      121 gtggatcaaa caaattcgag cccgacctct ctacagcagt cgctgacacg cctgcacgcg
      181 cagctgggcg ctgcgccgag gatggatgag acctccagac gtctattgcg tgaagtactg
      241 cgggatatcg agagggtatt gcaggaccag cagcccacgg tgcgcggccc gcagtcgcgt
      301 ctggaggaac tggcggtcag gtttgacgtc ggacatcccg cgctgtcggc cagcatccgg
      361 gaaatcgtgg atctgctggg tcgcgcggga ctctagttcc agggcccgcg cgaccctcga
      421 catcagcgat cgcgaggacg ctcagctcgc gccatgctgt gcccacttcg cctgcttggc
      481 ggcgatgatg gaggccagct gggtcttctg atcctgagtc aatacgccat agatctgctg
      541 ctggacggcg tcccgagcct ggatgcgtgc agtcatgcga gccagcaggt cctgcagagc
      601 agcgccatgg ttcgggtcac ccggattcgc cagcgctgca aagttcggtg cgccggcgct
      661 ctggtgctgt gctttcgcat tgttgcgcgc ggtcaacaga atgctgtgaa cgctctgctt
      721 ctgcgcatcg gtcagattga gctgatgcag tgtgtgcatc atcatgccgc ccatcatccc
      781 gccgcgatgc gggtgccagt gctggtgatt gccacctgtc gagctgtcac tgggagcggt
      841 gccagcggag ctgggagtcg tctgagcgta gacgccgccc gcgacgccgg tcagcgccag
      901 cgccaccatc aaccacgtaa ctcgattttt catatctcct gcctcaaatg agagtagccg
      961 tcagccatgc aacgcgccga cagcgcaagc gtggacaagg gcgggtcgga attggattaa
     1021 atttcgcacg gtgatagatg gctgaatgta actctatatt ttaattagat tttatgggtg
     1081 atcaccctgt ggatgacgac cgccgcaagc cggccgatct gggggtaacc caccgcgcgg
     1141 gcgcatagcc accctggcgc tcgtcaatga agctcgcgac ccagtggcgc aaatagggcg
     1201 aatcgaccgc cggattcgtg tgacgtgcgc cttactggtg cgcttgcggt aacgccgccg
     1261 gcgcgcgtgc gttaaaccta atgaggcaag gttcctaagc tgcggttttc gtggagcttc
     1321 gaccgcgaac ctatcaagcc acgcattcag gcacaggctt tgcagtaact cacccgaaat
     1381 gtgcgtctag ggttccggcc tgcatgccac gcaggtgtct ggtccgagag aggcagtcca
     1441 cgacaggcga gcgtggatac acggcgggac aaaagcccgg gagaactggt tgcggggagc
     1501 acggctgccc cgggacgctc tcctgccagg aggatctctc ggacgcggcg ctcgaatctc
     1561 cttgccagtg atggacagct ttacggctgt tgaggagatc ggattcaaat gagaacacgg
     1621 cagcgcgcga atctatcgaa ggctgggaat cttaccgcga caccagcacg agggtggctg
     1681 cgtcgacgcg agccagtcac tgcgctcacc gccgcggtgc tcaccgcact ctacggtgcg
     1741 cgtgcattgg ccgcagccgg tgatgccggc gacgacaacg tcacgccgac agcggccccg
     1801 acctcgctcg atgaagtgat cgtcaccgcc agccgcagag ccgtgcaggc tgaggatctt
     1861 ccgatcagca tcaccgccgt gacgggcgat tcgctccagc aggccggaat tcaggacatt
     1921 gcggatctgg cgcattcgat agccggtatc agctacgccg acaaaggtcc attcagcggc
     1981 gtgaatggct cgacactgat catccgcggc ctgaacagcg aggcgaccgc gggccagctg
     2041 gctttggcaa cgccagtcgt cccaccggtg gccacctatc tggacgatac gccgttgttc
     2101 ttcagtctgc ggctgcagga cctggatcac gttgaagtgt tgcgcggtcc gcagggcaca
     2161 ctctatggtt ccggatcgct gggcggcacg atccgtttcg tacagaacgc gccggatctg
     2221 agtggatttg acgccagggc cgaagtgacc ggcagcgaca ccgcgcgcac gcacgagccg
     2281 aacggcgatg tgaacggcat gctgaacttc gcgctgagcg atgcgctggc cgtccggctg
     2341 aacgcgggcc tgtcgtatga tgccgggttc atcaatcagc ctgacctgta tgtgttgaat
     2401 tcaaaagcgc agccgatctc ggcggatccg accaatcttc tgagccctcc gctcacctac
     2461 tctcgggccg gtaccaacga atacgaatac aagaatgggc gcctagcggt gctgtggaag
     2521 ccgaacgacg attttcatgc gcagctgtcc tatttctatc agttgtcgac cgcagacgga
     2581 ttcccgtacg actcggtgct gttcgggttg aacaacctgt cgtcctcgga tcacacgcag
     2641 gcgcatacca atgatcaggt gaatctggtt tcactgacgc tggaggagaa tttcggtttc
     2701 gcgacgctga cgtcgaacac ctcctggtcg caccacgtca atcgcaccga cagtgatctg
     2761 actgatctgt acgcggactt cccgttctat ccgtcgctat atggttccaa tccgcgggtg
     2821 ctcgttaccg gccacgacaa gctggacgac aagcctatca ccgaagaaat ccggctggcc
     2881 tcggcgaccg gcggcttctt cgactgggtg ggcggattgt tctataagca cgagaccact
     2941 gacattcagg agcacgagtt ctatcccggc tatttcgact acttcaacgc ctgcgtgccg
     3001 gtctacggcg tcagcaatgg cgacggcgtt accccatcca agtgcggcat tggtgagacg
     3061 gtgttcactc ccggcaccaa taccttcgtg gacggtattc cgattgtcga ggaccaggcg
     3121 tacatcggcg actttgaaac caagtacacc gatctggcgg cgttcggcga actgacggcg
     3181 catttgacat cggcatggag cgtcaccggc ggtgcccgcg ttttcaggca gaccgtggag
     3241 caggcgcagc agaccggact gctgttcgac gggccgatct cgatcgacaa caattccctg
     3301 agcaacgcct ggaacaaggc attgtggaaa ctcaacagct cgtatcagct cgacaagacc
     3361 aacctgatct acgccacctg gtcgcaggga tttcggcgtg gcggagtcaa cgcgctaccg
     3421 ccacaagagc cggcggtgaa ctatgtgacc aatcccggcc tgtacaaggt ctccccggat
     3481 accgccgaca actacgagat cggagtcaag ggcacgatcg ccaaccgatt ccgctattcg
     3541 gcggatatct tcgagattca atggcaccac gtgcaggagg gcgcacagct cacaccgctg
     3601 gtgctgccgg cgtccgtcaa tctgggtgat gcctacagtc agggattcga atccgaactc
     3661 tattgggcgc ccgacgatca tttcacggtg caggcggact acacctacga tcacacacgg
     3721 gtcagtacga tcacagccct ggcgataggc ggtttggccg tgccgccgcc gcttcccggc
     3781 ggtccgctgc ccggtacacc gaagaacagc gcagcgctga ctttgggata cgggcatctg
     3841 gatgcgggcc cgggtgcgtt tcgtgcagag ctgacagcgc actatcaaag ttcactgctg
     3901 cccgccgtgt cctccacgat cccgaccgta cctggctaca ctaccctcga agcgcgcttg
     3961 agctatcaaa tgccgcactg ggagctcacg ctcttcggca ccaacctcac gaacgtgctc
     4021 ggcatcaatt cgttctcgga tccaaccaac tatcagcagt tctatcaggc gctgatcacg
     4081 ccaccgcgta cgatcggatt gaccttggcg tattccttcc gcgagaagta aggctgcgcc
     4141 ccaaccggct agtgagtcgg aggatgcttg ctgtcgcagc ggtaggggat tgccggagcg
     4201 ctgaaagccg gacgacttca tgttccgaca actacgcggc ggtggtcgta gcatcctcgc
     4261 gcgccgcgcg cttgcgctcg tgctctttca ggaatcgctt gcggatacga atgttgtgcg
     4321 gcgtgacttc gaccagctca tcttcggcga tgaattccac cgcgtattcg agcgtgagct
     4381 gaatgggagg ggtcagcagg atcgcatcat ccttgccggc cgcgcggatg ttggtgagct
     4441 tcttggtctt gatcggattg accaccagat cgttgtcgcg actgtggatg ccaatgatca
     4501 tgccttcgta cagtttctct ccgggcgata cgaacatgcg accgcgctcc tgcagcgcaa
     4561 acagcgcata agcgaccgct tcgccgtcct cgttgctcac cagcacgccg ttgtggcggt
     4621 ccgggatgtc gcctttgacg accgcgtagc catcgaagat atggctcatg aggccggtac
     4681 cccgcgtcat cgtcatgaag tcgccctgaa agccgatgag tccgcgcgcc ggcacccggt
     4741 agtcgagccg cgcacggcca ctgccgtcga tcaccatgtc ggtcaattcg gcacgccgcg
     4801 tgccgagcgc ttccatcacc gcgccctgat ggtcctcgtc gatatcgatc gtcagcgctt
     4861 cgaacggctc gtgcacttcg ccatcgacga ccttcttgag tacctgcggc ttgccgaccg
     4921 caacctcata gccttcgcgt cgcatgttct cgatcagaat ggtcaggtgc agctcgccgc
     4981 ggccgagcac acgaaacacg tcggcatccg cggtatcttc gacccgcagg gcgacgttgc
     5041 tttgtaattc tcggtacagc cgctcacgaa tctggcggct ggtcacgaac ttgccttcgc
     5101 ggccggcgag gggggaggta ttgacctgga agttcatggc gagtgtgggc tcgtccactt
     5161 ggatcggcgg caggccctcg gcctgcagcg gatcgcaaat ggtcagaccg atggtgacat
     5221 cttcgatgcc ggtgatggcg acgatgtctc cggcgcaggc ctcttccacc ggtgagcgct
     5281 gcagtccggt gaatgtcagg atctgattga tcttcgcggt cccccgatcc tcatcgccgt
     5341 agcgcagggc gaccggtttg ccggccttga gggttccgcg gcgaatgcgg ccgatgccaa
     5401 tacggccgac ataagagttg tagtcgagcg tcgagacctg aagctgcagc gcagcgtcgt
     5461 gctgcgtcgg cggcggtggc acatactgca gaatcgcttc gaacagcgcg gtcatgtccg
     5521 gatcgcgccg ggtcacgtca gtgctggccc agccttgcag cgccgagccg tagatcacgg
     5581 ggaagtcgag ctgctcgtcg gtcgccccga gcttgtcgaa cagttcgaag gtctgatcga
     5641 ctacccagcc ggcccgggcg ccggggcggt cgatcttgtt gaccaccacg atcgccttga
     5701 gtccgagcgc cagcgctttg cgcgtgacga accgggtttg cggcatcggc ccctcgaccg
     5761 catcgaccac cagcaatacg ccatccacca tcgacagcac gcgttcgact tcaccgccga
     5821 agtccgcgtg cccgggcgtg tccacaatgt tgatgcgcgt gccgccgtat tcgatcgcgc
     5881 agttcttggc caggatcgtg atgccgcgtt cacgctcgat gtcgttcgag tccatgatcc
     5941 gctcaccgag cttctcatgc gccgcgaacg taccggactg cttcagcagg cagtccacga
     6001 gtgtggtctt gccgtggtcg acatgggcga tgatggcaat gttccgaatc ggacgctgca
     6061 tgcggctgac gggtatggaa aaagggcgtg gagggtagca gaactggggc gggtgctggt
     6121 aagatatttt tcggtctata taaagatatc tttatatgta gatgaattat gttcagagga
     6181 tcagtcgcca gtcaggatat cgaggaagtg gtgcgcgcgc tcaagagcgc ggcggagccc
     6241 agccgcttgc ggctgctgtg tctgttgagt gccgccgaat acaccgtcgg tgagctgtgc
     6301 acgatccttg gacagagtca gccgcgtatt tcacgccacc tgaggctgct caccgaggct
     6361 ggtctgctgg atcgcttccg tgagcagcag tgcgtgtact accgaatgcc agtcgcgggg
     6421 cgctattccc actgggttcg ccaggtgctg tcgatggccg atccggatgc caatgttctc
     6481 aagcgtgatc gggagcgggc gttgcgcgtc gtgggcgatc gcgcgcgggt ggcgcagcag
     6541 caggtcgaag ccgatgagct cgcgccgcgg gaggatctgg ccagcgtatt gcgcgaggta
     6601 ctcgggccta ccagcgtcgg cgagctgctc gacattggta ccggccacgg cctgatgctc
     6661 gagatcctgg ggccgatggc ccgtcatgcg gtgggcgttg atatctgcgc tccggcgctg
     6721 cgactggctc gaacccgcgt tcatggcgcg gggctggcgc acgtcgaatt ccgtcgcggc
     6781 gacatgtatc gcctgccgtt tgatgatgcc agtttcgata ccgtgacgat ccatcgcgtg
     6841 ctcgcctcgg ccgagcggcc gggcgccgcg atcggcgaag tcgcgcgcat cctgcgaccg
     6901 gatggccggc tgctcgtggt cgaggacttt gatttgatcg atgcgcgggg cggctccaac
     6961 ccgctgtctg cactgcgtcg ctggttcgat gcggccggaa tgagcattgg gcatcttcgg
     7021 ccatgcgatc tcggtggccg gcatttcatt atcgccttcg gccggcacgc ctcgtctgcc
     7081 tctcaacttt cacaaccgca acagcaagca ggtctcgcat gaatggctcg gctctgtctg
     7141 atcagatggg cagcgatctg agcgcgcttc ggcgcccggt gcagatttca ttcgaattct
     7201 tccctccggc agacgaaaag atggagcaga cgctttggga gtcgatccag cgtctggcgc
     7261 cgttgcggcc gagattcgtc tcggtcacct atggcgccga tggctccacg cgcgagcgca
     7321 cgcaccgcgc cgtgatgcgt gtgctgaacg aaacctcgtt gctgccggcg ccgcacctga
     7381 cctgcgtcgg cgcggatcgc gagcacgtgc tcgacatcgc gcgcacctac tggaaggcgg
     7441 gaatccggca tatcgtggca ctgcgcggcg atgcacccaa aggcatgagc gtctatcagc
     7501 cgcatccagg tggctttgcg tatgcggcgg acctggtgcg cggcctgaag acggtcggcg
     7561 attttgaaat ctcggtagcc gcctatccgg aagtgcatcc ggaagcaccc gatgcaccct
     7621 tcgatctgca gaacctcaag gccaaactgg acgcgggcgc cgatcgggcg atcacgcagt
     7681 tcttctttga ccccgaggtg ttcctgcgct ttcgcgaccg ctgcgtcgcg gcgggcattc
     7741 gcgcgagtct ggtaccgggc attctgccga tcacgcgctt tccgcagatg ctcaagatcg
     7801 cctcgcgctg cggtaccgcc gttcccaggt ggctgcacca gcgcttcgag ggcctggaag
     7861 aggatgccga tacccgcaag ctgatcggag ctgcggtcgc gattgcgcag gttgaggcgc
     7921 tgtcgcgcga gggcatcggg gagtttcatt tttacacgct caatcgcgcc gacctgagct
     7981 acgcgatctg tcatgcgctc ggcgtacgtc caacacgaac tgcatcactg actgaggaga
     8041 gcgcatgaac cgcgagcagc gcattcgcga gttccgtcgt ctgctgaccg agcgggtact
     8101 gctgctcgac ggcgcgatgg gcacgatgat ccagggctac aagcccgacg aggcggccta
     8161 tcgcggccag cgctttcatg actggcagtg cgacctgaag ggcaacaacg atctgctgac
     8221 gctgacgcag ccggccatga tccgtgagat ccacgccaag tatctggagg caggctccga
     8281 catcatcgag accaatacct tcagttccag cgctcccgcg cagggcgact acaagatgga
     8341 aggcctggtg agtgaactca atctcaccgc tgcgcgcctc gctcgcgagg tcgccgacag
     8401 ctatgccgaa cgcaccgggc gcgcgcgctt cgtcgcgggc gctctggggc cgaccaaccg
     8461 caccgcttcg ctgtcgccca acgtgaacga cccgggctat cgcaacatca cgtttgatca
     8521 gctggtcgcc acctacgctg aagcgacctc ggcattgatt gagggcggcg tcgatctgat
     8581 cctggtcgag accatcttcg atacgctcaa cgccaaagcc gcgttgtacg cggtacgacg
     8641 tacgatgcgc gagcgtggcg tggacctgcc aatcatgatc tccgggacga tcaccgacgc
     8701 gtccggtcgc acgttgtcgg gtcagacggt cgaggcattc tggaattcgg tgcggcacgc
     8761 ccgaccgatc gccgtcggcc tgaactgcgc gctcggcgcg aaacaattgc gtccgtacgt
     8821 ggaggaattt tcccgcatcg ccgacatcgc gatctgcgcc tatccgaacg ccggactgcc
     8881 caacgctttc ggcgagtacg atgagcaggc gtgtgagacc gcggagctgg tgggcgaatt
     8941 cgccgcggcg ggcctggtca acatcgtcgg cggctgctgc ggcacgacgg ccgatcacat
     9001 cgcgcacatc ggcgaggccg tcggatcgct cgcgattcgg gtgccgccga aactgcagcc
     9061 acgatgtcgg ctggcaggtc tcgaggcgct cgacatcgga ccggagagcc tgttcgtgaa
     9121 cgtcggcgaa cgcaccaacg ttaccggttc ggccaagttc cgcaaactga tcgaggcgga
     9181 cgactacaac gccgcgctcg atgtcgcccg cgcgcaggtc aatagcggtg cgcagatcat
     9241 cgatatcaac atggacgagg gcatgctgga ctcggaagcc gccatggtgc gcttcctgaa
     9301 tctgatcgct tcggagccgg atatcgcccg cgtgccggtg atgatcgact cttccaaatg
     9361 gtcggtgatt gaggccggac tcaaatgtct gcagggtaag ggcatcgtca actcgatcag
     9421 cctcaaggag ggggaagcgc agttcctgga gcgtgccggc caggtgcgtg attacggggc
     9481 ggccgtggtc gtcatggcgt tcgatgagaa aggccaggcc gacacggtgg agcgtaaggt
     9541 ctcgatctgt gagcgctcct acaacctgct gacgcagaag ctggactttc cgcccgaaga
     9601 tattattttt gacccgaaca ttttcgcggt cgcgaccggt atcgaagagc ataaccgcta
     9661 cgcgctcgac ttcatcgagg ccaccgcgca gatccgggag cgatgcccgc acgcgatgat
     9721 cagcggtggc gtcagcaacg tttcgttctc tttccgcggc aatgagccgg tgcgcgaagc
     9781 gatgcattcg gtgttcctgt atcacacgat tcgcgccggg atgacgatgg gtatcgtcaa
     9841 cgcggggcag ctcgcgatct atgaacagat cgagccggag ctgcgcgaac gggtcgagga
     9901 cgtgatcctc aatcgccgtg cggatgcgac cgagcgactg ctcgagatcg cgcagcgcta
     9961 caagggtgag tccggcacca ggcgtgccga agacctggcc tggcgaaact ggccggtgac
    10021 caaacggctg gaacacgcgc tggtacgcgg gatcgatgag tatgtgatcg aggataccga
    10081 ggaggcgcgc agccagttcg accgtccact gcaggtcatc gaaggcccgc tgatggatgg
    10141 catgaacgtg gtcggtgacc tgttcggcgc cggcaagatg tttctgccgc aggtggtcaa
    10201 gtccgcccgc gtgatgaaaa aggccgtcgc acacctgatt ccctatatcg aaaaggaaaa
    10261 ggcgagcaga ggtgtcgcgc tgggaaccaa cggccgcatc gtgatggcga ccgtcaaggg
    10321 cgatgtgcac gacatcggca agaacatcgt cggtgttgtg ctccagtgca acaacttcga
    10381 ggtgatcgac ctcggtgtca tggtctcgtg cgagaagatc ctcgaggccg cgcgccgcga
    10441 gcgcgccgat atgatcgggc tgtcgggtct gatcacgccg tcgctggatg agatggtgca
    10501 cgtagccaag gagatgcagc gccaggggtt cacgcaaccg ctgttgatcg gtggcgcgac
    10561 cacctcaccg gcgcatacct cggtgcgtat cgatccgcag tatcaggggc cggtggtgta
    10621 tgtgaaggac gcttcgcgct ccgtcggcgt ctgccagcaa ctgattgcgg cgggcacgcg
    10681 cgagacgttc accgcgcggg taaagtccga gcacgcgctg cgccgctcac aacacggtgc
    10741 acggcgtacc aagggacccc agctgacgct ggcacaggcg cgcgcgaaca aggtgcgcga
    10801 ggactggcag agctatgcgc cgccggtgcc gcgcatgctc ggcctgcgaa gctttgagaa
    10861 ctattcgatc gaggagctgt cgcgttacat cgactggacc ccgttcttcc aggcgtggga
    10921 gctgcgaggt agattccctg acattctcac cgatgcgcag tttggcgagc aggccagcag
    10981 tctgtacgcg gacgcgcgca agatgttgcg caccgtgatt cgcgaacaat ggctgcaggc
    11041 gcgcgcggtg atcggcctct tcccggccaa cgccatcgat gaggacctgg aactgtacga
    11101 ggatgagact cgtgggcggg tgattgcgcg gctgcacttc ctgcggcagc agaaggacct
    11161 gccggaaggc aagccacacg gagcgctggc cgatttcgtc gctcccaaga gttccggagt
    11221 ggccgactac atcggtgcct tcgcgctcac ggcgggcatt ggcatcgagc cgcatttgca
    11281 gcgcttcgag gccgcgcacg acgactactc cagcatcatg ctgaaagtac tggcggatcg
    11341 tttggctgag gccttcgccg agcggctgca cgagcgcgta cgacgtgagt tctggggtta
    11401 tgcgccggac gagaagttgg gcaatgagca gctcatccgc gaggagtatc gcggtatccg
    11461 gccggcgccg ggatatccag cctgcccgga tcacaccgaa aaggccacgc tgtggaaact
    11521 gctggaggtc gataagcgtg ccgctatcac gctgaccgac tcgtacgcga tgtatccgac
    11581 cgctgcggtg agcggctggt acttctcgca tccgcagtcg cgctacctgg gggtgggtcg
    11641 catcgaccgt gatcaggtcg aagactatgc gcgccgcaag ggcatgagta ttgaagacgt
    11701 cgagcgctgg ctttcgccca atctggggta cgaaccggag ccgagcgcgc tgctgatcgc
    11761 atgaaccggc acgaggcgca tctggccgcg cccttaagcg ccgcgggcgg tcgcaagtcc
    11821 gccgccgggg aggcggaggt gacccgcagc ttctttggcg tgttccgcta cagtcggcgt
    11881 gcgattgcgc tggtgtggga caccaaccgc ctgctgctgt tcgcgctggc gatactgacc
    11941 ctgatcgcgg gtctgctgcc ggcaagcatt gcctgggtcg gagctcagat cgtcgatgcg
    12001 gtggtcactg cccgcaacgc ggccgatcac aatgcaatgc gggtgttgcg cctggtattg
    12061 ctggaagggg tgctggtggc ctcggtcgca ggggctcagc gcggtctgtc actggcgcag
    12121 gcgctgctgc gggcgcagct cgggcagcgc gtgaacgtca tgatcctcga gaaagccctg
    12181 acgctggagt tgcgtcactt cgaggactcc gaattctacg acaagctcac gcgcgcccgg
    12241 cgcgaagcct cgagccgccc attgagcctg gtgatccgca ccttcggctt gggccagaac
    12301 gccatatcgc tgctgagcta tgccgcgcta ctgatccgct tctcgccgtg gaccatggtg
    12361 gtgctgctgc tggcgggact gccggcgttc atcgcggagg cgaagttctc cggcgacgcg
    12421 ttccgcctgt tccgctggcg ctcgcccgag acgcggatgc agatctatct ggagaccgtg
    12481 ctcgcgcgcg aggatcacgt caaggaagtc aaactgtttg gcttaggtgc gcgtctgttg
    12541 cagcgctacc gcgacatctt tacccggctc tacggcgagg accgcgcgtt gtcgatccgg
    12601 cgcgacagct ggggatttgg actgggcctg cttgcgacca cggcgttgta tggtgcctac
    12661 gcgtggatcg cgctgtcgac cattcgctcg ctcattacgc tggggcagat gacgatgtac
    12721 ctgctgctgt ttcgtcaggg acagtccgcg gtggccgcga tcctgtcctc ggtcggcggc
    12781 atgtatgagg acaatctcta tctgtccacg ttgtatgagt atctggagac accggtcgcc
    12841 ctgtcttcag gtcacacggc gcgcggccct ttgccttcgg acggcgtgcg gttcgagcag
    12901 gtcagtttta cctatcccgg tgcctccgaa cccgcgctgg tggacgttac tctgcatctg
    12961 atccccggca gcagcgtcgc gctggtgggc gagaacggtt cgggcaagac caccctgatc
    13021 aaattgctga cgcgactcta cagtccggac tcaggccgga tactgctgga tggtctgccg
    13081 ctgccgcagt gggaccagga tgccctgcgt gcgcgcatcg gtgtgatttt tcaggacttc
    13141 actcgctacc agatgttggt gggagagaac gttggagcgg gggatgagcg ctatttcgaa
    13201 gatgaacagc gctggcgcga cgcagcgggc aaggggcagg ccgctgattt catcgagcgc
    13261 ttgccgggac gctatcagac gcagttggga aaatggtttc agaacgggca ggaactatcg
    13321 ggcggacaat ggcagaagat cgctttggcg cgcgccttca tgcgtaccga agcggacatt
    13381 ctggtgctcg acgaacccac tgcggcgatg gatgcgcggg cggaggcgca gatcttcgaa
    13441 cacttccgca cgctcgcccg cgggcatatc acgattttga tctcgcatcg cttttccacg
    13501 gtgcgcatgg ccgatcagat cgcagtcctc gatcgtggtc ggatcatcga gaagggctcg
    13561 catgaacagc tgatggcgct cggtggccac tacgcgcagc tgttttcgct gcaggcgcgc
    13621 ggctatcgct gatgtttgtc ggtggatacg cgctacaatg gagtcatctg gggatgaagc
    13681 tatcaacaat ggaagaaacc accgccgata ctttcgattc cgccttctcc aaggcagtcg
    13741 atcttggcaa ccgactcgcc ggtaaggaca aggaagccga tctttgggat attgctgatg
    13801 gtttgttggc cggcgcgatt cagtactggc tctactcgcg ccagccctgt ggcgacccgc
    13861 gctgtgagga ctgtgtcccg atcagcacgg ccgatgcacg catggcggag ctcaagcgcc
    13921 tgatcgatca gctcgcggcc gagagcgagt actttcacgc cccgaccgac gccaacgccg
    13981 gccgggcctg aaccggctgc agtgcctgcc taagcctcgg cggtcgcggg cagccgcgtg
    14041 acctccgcga gcgcggcctc gagccgcgac aatccttccg tcagcagcgc gtcactgatc
    14101 gtcaacggtg ccaggaaccg gatcacgttg ccgtaggcgc cgcacgacag aatgatcagt
    14161 ccctgacgtg cggcggcctg taccagtgcg cgcgtcaggt cagcatccgg ctccgcggcc
    14221 gaaccgttcc tgaccagctc catcgccacc atcgagccca caccccgcac atcgccgatg
    14281 tggtggtacc tggcctgcag cttgcgcaaa cctgcgatca gcgcgctgcc gagctgctgc
    14341 gcgcgcgcca acaactgctc gtcgcggatc acatcgagca ccgcgagagc cgcggcgcag
    14401 gccaccgggg agcccgcaaa ggttccgccc agtcctcctg gcggcggcgc atccatgatg
    14461 gcggccttgc cgatcaccgc ggacagaggc aagcctccag cgatgctctt ggcgacgacc
    14521 atcaggtcag gctcgacgcc ggagtactcg aacgcaaaca tgcggccggt gcgcgcaaac
    14581 ccagattgga tttcatcggc gatcagcagg atcccgtggc ggtcgcacag tgcgcgcaag
    14641 cgctgcagaa actccgccgg ggcgatgtag aaaccaccct cgccaagcac cggctcaatg
    14701 atgaccgctg ccacgcgctc cgggtccact tccgatttga acaactgttc gattgccgcc
    14761 aggcaatcct ggggcgtgac agcgtggtag gccatcggga acggcacgtg aaacacatca
    14821 ggcagcagcg gtccgaagcc ggccttgtag ggttgcacct tgccggtgag cgtcaaggca
    14881 gcgaatgtgc gtccgtgaaa accagcgctg aacgcgatca gcgccgatcg tcgcgtgtag
    14941 tagcgtgcga tcttcagcgc attctccacc gcttcggcgc cggtggagaa cagcagggat
    15001 tttttcgccg tcggccccgg cgtgatcgca ttgaggcgct cggccagtgc gacatagccc
    15061 tcatacggga tgatctggaa gcaggtatgc gagaagcgct gcaactgctg ctcgatcgcc
    15121 gtcacgacgc gcggatgcag gtgacccgag ttcagcaccg cgatgccgct ggcgaagtcg
    15181 atgaactgcc ggccttcgac gtcccagatc tccgcgttgc gcgctcgctc gatgtagatc
    15241 gcgtgggcgt tggacacgcc tcgggcgaca gcggcaccgc gccgtcgatg cagctcggaa
    15301 ttgtcactca tgcgcagttc ctcctgatcg gggcactata acctcataac cggttttgtt
    15361 tgccctcgac gctgtcgcat cgctaaaatt ccaaagtttt gcagcccttt atccgtgagc
    15421 gtaacacctt gagcgaacaa cccatcacga ccgaagtggt catcattggc gccggtcctg
    15481 ccggcctgtt tcaggtattt gagctgggct tgctgggcat caagtgccac atcatcgata
    15541 cattgaaggc cccgggcggt cagtgcaccg agctgtatcc ggacaagccg atctatgaca
    15601 ttccggcgat accgatgtgc ggtgcgcagg agctggtgga ccggctgatg cagcagatca
    15661 aaccgttcgg cgcgcagttt catctcggcg aagagacggt ggagctgaag aagcaaccaa
    15721 gcggaa
//
LOCUS       DATMRM010000295        12905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1415938, whole genome shotgun sequence.
ACCESSION   DATMRM010000295 DATMRM010000000
VERSION     DATMRM010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12905
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415938"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VNZ06_08680"
     CDS             <1..551
                     /locus_tag="VNZ06_08680"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWW20865.1"
                     /translation="AGVELQFLPTDFIQVNAAVNRDLVSAAVRLLQPESDSQVLDLYC
                     GLGNFSLALARRAAHVVGVEGDTVLVERARTNARRNQIANAEFHSTDLSADLSHASWL
                     RRSYSHVLIDPPRIGAREVLPVVARLAPARLLYVSCHPATLGRDVGILVHEHRYRLLA
                     AGVVDMFPHTAHVESMALLAAC"
     gene            561..1052
                     /locus_tag="VNZ06_08685"
     CDS             561..1052
                     /locus_tag="VNZ06_08685"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HWW20866.1"
                     /translation="MAIEIERKFRVADDSWRGQIQSSTLLRQGYLANTDRASVRVRLA
                     GEQGWLSVKAMTPGRARAEYEVAIPMTDAGEMLDRLCEGPLIEKWRHIVIHDGDRWEI
                     DEFLGDNAGLVIAELELDCEHERFASPPWLGDEVTHDERYYNFRLSQQPWRCWPENLQ
                     AGR"
     gene            1049..2119
                     /gene="nagZ"
                     /locus_tag="VNZ06_08690"
     CDS             1049..2119
                     /gene="nagZ"
                     /locus_tag="VNZ06_08690"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HWW20867.1"
                     /translation="MSLGPVMLDIAGTALTAADREILSHPLVGSVILFTRNYVDPQQL
                     QGLVADIQSVRSPALLVSVDHEGGRVQRFRPGFSALPPLRRIGQAFDGDAAKGLEMAR
                     ALGWLMACEVLACGLDFSFAPCVDLDYGVSSIIGDRSFHARAPIVSQLAIAYMHGMRD
                     AGMAATAKHYPGHGAVVADSHHALPVDRRELVDMDEDLLPYRRLIANGLPAIMVAHVL
                     YPAVDDVPASFSRHWIGGVLRQELGFAGLVFADDLTMAGAAAAGDLLQRAQRALAAGC
                     DVLPVCNDRPGVERLLGSLRASADPASQLRLVRMRGRKQLRFENLTASAAWQSAREWL
                     AHSAASPPELSFKPGGSDAGGA"
     gene            2126..3058
                     /locus_tag="VNZ06_08695"
     CDS             2126..3058
                     /locus_tag="VNZ06_08695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWW20868.1"
                     /translation="MTQWSVDFLRGVLDAAPEGIVVCESRQADQLAVYVNSAFEKLAG
                     FSREELIGQDLRRLQAWDREQEGRVQLREAITRGESARALMRNYRKDGSAFWNEVFIQ
                     PLRGADGKVTHYVSFHRDVGERDRTGTRRISGLATWLREDRLSGLCSRAYFEELLQHD
                     WQVGVRESRQLTLLAFDICDLAAYNDTFGRSAGDACIRRVAGIIGASFKRGADVVARW
                     DGGCILALVRSGDLSLVPAFASSVAQKVLDQHIHHPRSSRQKFVTVNVAAASLNPLAE
                     KPCDVLIQATMKALKRAKEDREGRVAIARPEELA"
     gene            3055..3540
                     /locus_tag="VNZ06_08700"
     CDS             3055..3540
                     /locus_tag="VNZ06_08700"
                     /inference="COORDINATES: protein motif:HMM:NF021438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HWW20869.1"
                     /translation="MSTRFIARGLALCALAVLPEPGSRAAAPPEGQAASLIGRWLTDP
                     PDGIIEISLTPDGSYQGRIIGGDNPYRIDSKNPDASKRSARLLGQVIVQDLKYESLGH
                     WSGSSIYDPDSGHTYSCHLELLEANKLKVRGYLGVSLLGRTQIWTRYTGTSLVLPKVA
                     H"
     gene            complement(3572..4183)
                     /locus_tag="VNZ06_08705"
     CDS             complement(3572..4183)
                     /locus_tag="VNZ06_08705"
                     /inference="COORDINATES: protein motif:HMM:NF022425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsiV family protein"
                     /protein_id="HWW20870.1"
                     /translation="MKRIPIWLLCLAITLCLPAHAAAPAAAGAQGASPYYDVEIIVFR
                     ASSPGADEDWTAPVPGRGFGSTATRGGAAPAVVKVLGATDYHLNTLETALKNSGAWRP
                     IAHAAWIQTAANWGTHTGFALSDLGINVPGLSGTVYVERGTYIHLGFEIQYESGAGPT
                     YTINEMRSVKYNEKQYFDHPAVGIIALVSPIKRGSDATMPTAH"
     gene            complement(4194..7664)
                     /gene="mfd"
                     /locus_tag="VNZ06_08710"
     CDS             complement(4194..7664)
                     /gene="mfd"
                     /locus_tag="VNZ06_08710"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007966.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HWW20871.1"
                     /translation="MPPTTTLLHPPLPLPGDAASRWRGLHGSARSLALSEAVAADSRP
                     WVVIAADARELERLADELRFFGGDALTILTLPDWEVLPYDVFSPHPDIVSQRLLTLSR
                     LPQLTRGILLLSADSLLARLPPVSYVQARSFELKTGAALAIEPLRTRLIESGYASVSQ
                     VTSPGEFALRGSLFDVYPMGTDTPVRVDLLDDRIDSIRSFDPDTQRSLESIQQLKLLP
                     AREMPLDAESVKNFRRRYRARFEGDVARMPIYRGISEGIAPPGIEFYLPLFFEATAHI
                     SDYLPRDMVIASDLDVEQAIEQAWDGITARYEDRRHDIERPLLPPPDVFVEPGRVHET
                     LAAHPTVGIERFSSADLEHVAAVHDFRTAAPPEFRLDARAAQPLAPLTGFLDTYVGRV
                     LIAADSPGRREVIAEMLGAHGRRPRALRGWEAFTQGDAALAICVAEDLRGLALEQPAL
                     MLLGESQLFGTRARQERRRRRTSASDPAVILRDLQSLEPGAPVVHETYGVGRYVGLQL
                     MEIGEQSGEFLTLEYRDGDRVYVPVHALHLVNRYTGGAPESAPLHKLGTDQWARARRR
                     AAEAVRDVAAELLDLHARRAAHRAASLNAGELEYQTFANGFPFEETADQAEAIRQVLK
                     DMQGTTPMDRVVCGDVGFGKTEVAMRAAFVAVQAGRQVAVLVPTTLLAQQHAQSFRDR
                     FADWPVRIEALSRFRSSGETATVLDGLERGSVDIVIATHRLLHADARFQNLGLIVVDE
                     EHRFGVRDKERLKTMRAEVHVLTLTATPIPRTLNMALGGLRELSLITTPPAARLAIKT
                     FITEWHNATLREAALRELRRGGQVYVVHNEVQSIDKVAAEFAALVPEAAVRVGHGQMR
                     ERELEQLMVDFYHRRFDVLVCTTIIESGIDVPSANTILINRADRLGLAQLHQLRGRVG
                     RSHHRAYAYLIVPSRRGLTGDAAKRLEAIESLEDLGAGFVLATHDLEIRGAGEFLGEA
                     QSGQLSEVGLSMYLEMLEQAVKAMRENREPALERPLAAISEIELHVPALLPQDYVPDV
                     HLRLALYQRIAAADGSALQDMTAELIDRFGSLPEVANNLLRLANLRLTARELGVRRLD
                     LGSAGGSVQFEEHNRVDPSRVIALIQRQAKEFRLEGPLKLRITRSLPKSDQRFDYAQQ
                     LLTKFTTDSR"
     gene            complement(7730..8053)
                     /locus_tag="VNZ06_08715"
     CDS             complement(7730..8053)
                     /locus_tag="VNZ06_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20872.1"
                     /translation="MTEKSSFPLTLAVGLAAIVAGGMVSVARADLVVTDDQVSVIPSD
                     IARPSAGMTMQKVEEKFGAPIERHDAVGAPPITRWDYTNFSVFFEHDRVIHAVVTGAP
                     AAPSP"
     gene            8192..9439
                     /locus_tag="VNZ06_08720"
     CDS             8192..9439
                     /locus_tag="VNZ06_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HWW20873.1"
                     /translation="MFQPLSVFIGLRYVRSRQHKFFVSLITWVSLAGVGLGVAALIVI
                     LSVMNGFEGDLRSTLLSLSAHARVTAQGVPAADANAALAARLRRLPGVVGVAPYIDLT
                     ALVVHQTEMQPITLRGVDPAQETAVADVRPYLVEGDYSTLEAGSNAVILGRDVADQLG
                     VRVGDAVTVLVPMVSGDRMPEPRLRQFTVGGVFSSELPDHDNTLVLADIDDVRAFAPQ
                     VEGASGLRLRFTDAMQVTLYMPAVRAATGSGFEISDWTQDNASYFRAIRIEKTMMSLI
                     LLLIVAVAAFNIVAMLVMVVNDKRTDIAILRTLGASPGSVLRTFMTQGLVIGWFGVGV
                     GVLLGVLLASHVTSIVPALESLFHFQLLDSNVYYNTDIPSQLRTRDVLWISLAALILT
                     LCSTLYPAVRAARTPPADALRYE"
     gene            9452..10693
                     /locus_tag="VNZ06_08725"
     CDS             9452..10693
                     /locus_tag="VNZ06_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HWW20874.1"
                     /translation="MSYEWLIGTRYVRSTRGRGFLSFITVVSASGLAIGVAVLIVVLS
                     VMNGFGRELRTRILSVTSHATLTGLEGTLEDWRQDQAAALKLPGVIAAVPYIEEQALL
                     AHGARTAGTAVRGVEPEQERRAVGLAEHIQGGRLEALTDGGYRIILGDALADALSVKV
                     GDEVVLIAPRGNPTPVGIEPRMRRFRVAGIFHSGMYKYDRGLALLNLHDAARIYQLGD
                     AVTGVRLALRDPFAAPRTVHALALALGGGFYVSDWTRIESTLFRSIEITKSMLFVILL
                     LVVAVAAFNIVAALVMVVKEKRSDIAILRTLGAGPRNVLAAFSIQGGFIGLAGTLAGA
                     GLGLLLSANLESLVHGLEHLLGTHFLDAKVYLMSDLPGYAEPGDVLRICAVAFALCAL
                     ATLYPAWRAAGIQPADALRHD"
     gene            10686..11393
                     /locus_tag="VNZ06_08730"
     CDS             10686..11393
                     /locus_tag="VNZ06_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWW20875.1"
                     /translation="MTEGKPSPPVVLAAHAVCRSFREGSSTLEVLRDVELSVAAGERL
                     AIIGVSGSGKTTLLQILGGLDRPDRGSVQVLEQDIHALSETERGALRNRAIGFVYQFH
                     HLLPEFSALENVAMPLLVRRERTPVAIARARDILTRVGLAERLTHRPSQLSGGERQRT
                     AVARALVSSPSLVLADEPTGNLDGRNASQVFELMLELNRELGTSLVVVTHDSRLAARM
                     DRVLELSSGALLQLASP"
     gene            complement(11375..11914)
                     /locus_tag="VNZ06_08735"
     CDS             complement(11375..11914)
                     /locus_tag="VNZ06_08735"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HWW20876.1"
                     /translation="MPRRLVKQFVGFLHARRDRWYLSIFGDRLTDSHLWSLNRHSITA
                     AFGVGIAVSFIPLPVHLPLVVAICLWSRLNVAVGVAACYIVNPFTMVPIYYMAYRIGA
                     WLLRYRAHHFEFQLTWNWLEHGLGPAWRPFLLGCLVCGITFGLLGRYALEIGWRMATL
                     HKYRVRRERRRARAYGLAS"
     gene            12096..12701
                     /locus_tag="VNZ06_08740"
     CDS             12096..12701
                     /locus_tag="VNZ06_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWW20877.1"
                     /translation="MWPIILCSIVAAAIVLERIWTLQDRRVLPPDLTKKVWKLVESHR
                     IDDKIVAALEQNSPLGKLLAVGLANRDRPREVLMERLEDTGRHVVHELERFLNTLGTI
                     AGVSPLLGLLGTVTGIIKAFNAINAGGAGDPRMLSGGIAEALIATAAGLCVAIPSLVA
                     YRYLRGRVDRIVVEMEKHALRLADAVESASRRGSEPRAVNS"
     gene            12711..>12905
                     /locus_tag="VNZ06_08745"
     CDS             12711..>12905
                     /locus_tag="VNZ06_08745"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWW20878.1"
                     /translation="MKMKPRRSEEPEINVVSLIDVVLLLVVFFMLSSNFTAEGRLQIR
                     LPQANSGPTSRNSDEGLVVTV"
BASE COUNT         2234 a         3947 c         4310 g         2414 t
ORIGIN      
        1 atgcgggcgt cgaactgcag tttctgccga cggatttcat acaggtcaat gcagcggtca
       61 atcgtgacct ggtcagtgcg gccgtgcggc tgctgcagcc cgaatccgat tcgcaggtac
      121 tggacctgta ctgcgggctg ggtaatttct cactggcgct cgcgcggcgg gcggcccacg
      181 tggtcggtgt cgaaggcgac accgtcctgg tcgagcgtgc gcgcaccaat gcccgtcgca
      241 accagattgc caatgccgag tttcacagca ccgacctgag tgctgatctc agccacgcct
      301 cctggctcag gcggtcctac agccacgtgc tgatcgatcc gccgcggatt ggggcacgag
      361 aagtgctacc ggtggtcgcg cggctcgccc ctgcgcgcct gctgtatgtg tcgtgccatc
      421 cggcaaccct gggtcgcgac gtgggaatcc tggtccacga gcatcgctac cggctgctgg
      481 ctgccggggt ggtggacatg tttccccata ccgcccacgt ggaatccatg gcactgctgg
      541 cggcttgctg aagacatcgc atggctatcg agatcgagcg caaattcagg gttgccgatg
      601 acagctggcg cgggcagatt caatccagca cgctgctgcg gcaggggtat ctggccaata
      661 ccgatcgtgc ctcggtgcga gtgaggcttg ccggggagca gggctggctg tcagtgaagg
      721 ccatgacgcc gggacgggct cgggcggaat acgaagtggc gatcccaatg acggatgcgg
      781 gtgaaatgct ggaccgcctg tgcgaaggcc cgctcatcga gaaatggcgt cacatcgtca
      841 tccacgatgg cgatcggtgg gagattgatg agtttttagg agacaatgcg ggtctggtga
      901 tcgcggagct ggagctggat tgcgagcatg agcgatttgc ttcaccgcca tggctggggg
      961 atgaagtgac gcacgacgag cgttactaca atttccggct gtcgcagcag ccgtggcgat
     1021 gctggccgga aaatctgcag gcgggtcgat gagcctgggt ccggtgatgc tggacattgc
     1081 gggcacggcg ctgacggctg ccgaccgcga gatcctgtcg cacccgctgg tgggcagcgt
     1141 gattctgttc acgcgcaact acgtcgatcc gcaacagctc cagggactgg tggctgacat
     1201 tcagtcggtg cgcagcccgg cgttgctggt gtcggtggat catgaaggcg ggcgcgtgca
     1261 gcgcttccgc cctggattct cggcgctgcc gccgctgcgg cggataggac aggcattcga
     1321 cggcgatgcg gccaagggcc tggaaatggc acgcgctctg ggctggctga tggcttgcga
     1381 ggtgctggcg tgcggcctgg acttctcatt cgcgccctgc gtggatctgg actatggcgt
     1441 ctcgagcatc atcggtgatc gctcgtttca cgcccgcgcc ccgatcgtca gccagcttgc
     1501 gatcgcctac atgcatggca tgcgcgacgc gggcatggct gccaccgcca agcactatcc
     1561 gggacacggc gcggtggtgg cagactctca ccacgcactg ccggtggatc ggcgcgaact
     1621 ggtggacatg gatgaggatc tgttgcccta ccggcgtctg atcgccaatg gactgcccgc
     1681 catcatggtg gcgcacgtgc tctatccggc ggtcgatgac gtaccggcga gtttctcgcg
     1741 acattggatc ggcggggtgc tgcggcagga gctgggattt gcgggcctgg tattcgccga
     1801 tgatctgacc atggcggggg cggccgccgc cggcgatctg ctacagcgcg cacagcgcgc
     1861 cctggcagcc ggatgtgacg tattgccggt ctgcaacgac cggccgggtg tcgaacgact
     1921 gctgggtagc ctgcgagcct cggcggaccc cgcgtcgcag cttcggctgg tacgcatgcg
     1981 gggccgaaaa cagctccgat ttgagaacct gaccgcatct gcggcgtggc aaagcgcgcg
     2041 agaatggttg gcacacagcg cggcatcgcc gccggagctg agcttcaaac cggggggtag
     2101 tgacgcggga ggagcataag aaccaatgac gcagtggtcg gttgattttc tgcgcggtgt
     2161 gctcgatgcc gctcccgaag gtattgttgt ctgcgagtcg cgacaagccg atcagcttgc
     2221 agtttacgtt aacagcgcgt tcgagaaact ggccggattc agccgcgagg agctaatcgg
     2281 tcaggacctg cgccggctgc aggcctggga ccgggagcag gaaggacgcg tgcaactgcg
     2341 cgaggcgatc actcgcggcg aatctgcccg tgcgctgatg cgcaactacc gcaaggatgg
     2401 gtcggccttt tggaatgaag tgttcattca gccgctgcgc ggtgcggacg gcaaggtcac
     2461 ccactatgtg agttttcatc gcgacgtcgg cgagcgcgac cgcaccggca cgcggcgcat
     2521 ttcgggactt gcgacctggt tgcgggaaga ccggctttcg ggtctgtgtt cacgcgccta
     2581 cttcgaggag ctgctgcaac atgactggca ggtcggagtg cgtgaatcgc gccagctcac
     2641 gttgctcgca ttcgatatct gcgatctggc cgcctataac gacacctttg ggcgttccgc
     2701 gggcgatgcc tgcatccgcc gcgtcgccgg catcatcggt gcttcgttca agcgtggcgc
     2761 ggacgtggtg gcgcgctggg atggaggttg tatccttgcg ctggtgcgca gtggcgatct
     2821 gtcgctggtg cctgccttcg catcctcggt ggcgcaaaaa gtgctcgatc aacatattca
     2881 tcatccacgc tcgtcgcgtc agaaatttgt gacggtcaac gtcgcggcag ccagtctgaa
     2941 tccgctggca gagaaaccct gcgatgtgct gattcaggcg accatgaagg ctttgaagcg
     3001 ggccaaagag gatcgcgaag gacgggtcgc gatcgcacgt ccggaggagc tggcatgagc
     3061 acgcgcttca ttgcgcgtgg tctcgcgctg tgcgccctgg cggtgttacc cgagcccggt
     3121 tcccgggctg cggcgccccc agaggggcag gccgcgtcgc tgatcggccg gtggttgacg
     3181 gatcctccgg acggcatcat cgaaatcagt ctcacgcccg acggcagcta tcagggaagg
     3241 atcattggcg gcgacaatcc gtaccgcatc gacagcaaga atcccgatgc ttccaagcgc
     3301 tctgcgcggt tgctgggcca ggtgattgtg caggacctga aatacgagag tctcggtcac
     3361 tggtcgggta gcagcatcta cgatcctgac agcggtcata cctacagctg tcacctggaa
     3421 ctgctcgagg ccaacaagct caaggtgcgc ggctatctcg gcgtgtcact gctgggccgc
     3481 acgcagatct ggacgcgcta caccggcacg agcctggtgc tgccgaaagt cgctcactga
     3541 ccctcaggga tcgagcgatc ggcgtgacgg atcagtgtgc ggtgggcatc gttgcatcgc
     3601 tgccgcgctt gatgggtgag accagagcaa taatgccgac ggccgggtga tcgaagtact
     3661 gtttctcgtt gtatttgacg ctgcgcattt cgttgatggt gtaggtgggg ccggcgccgg
     3721 actcgtactg tatttcaaac cccaagtgga tataggttcc gcgttccaca tagaccgtcc
     3781 cgctcaaacc cggcacattg atgcccagat ctgagagcgc aaagccggtg tgtgtgcccc
     3841 agttggccgc tgtctgaatc caggccgcat gggcgatcgg cctccacgcg ccgctgttct
     3901 tcaacgcggt ctccagtgtg ttcaggtggt agtccgtggc gccgagcact ttgacgacgg
     3961 caggcgcggc gccgccgcga gtggcggtgc tgccaaagcc gcgcccgggc accggtgcag
     4021 tccagtcctc atcggcgccg gggctcgacg cgcgaaacac gatgatctcg acatcatagt
     4081 acggagaggc accctgagct ccagccgccg cgggagccgc ggcatgcgcc ggcagacaga
     4141 gcgtgatcgc gaggcacagc agccagatcg gtatgcgctt catgcggcct atcctacctt
     4201 gagtccgtag tgaatttcgt cagcaactgc tgcgcgtagt caaagcgctg atcggacttc
     4261 ggcaatgagc gcgtgatgcg cagtttgagc ggaccttcga gccgaaactc cttggcctga
     4321 cgctgaatca atgcaatcac gcgcgaggga tccacacggt tgtgctcctc gaactgcacg
     4381 ctgccgccgg ccgaacccag atccagacgc cgcacgccca gttcgcgtgc ggtcagccgc
     4441 aggttagcca gccgcagcag gttgttggcg acttccggca gactgccgaa gcgatcaatc
     4501 agttccgcgg tcatatcctg caacgcgctg ccatcggccg cggcgatgcg ctggtacaac
     4561 gccagccgca ggtgaacgtc cggtacatag tcctgcggca gcaacgcggg tacgtgcagc
     4621 tcgatttcgc tgatcgcagc cagcgggcgc tccagcgccg gttcgcgatt ctcgcgcatg
     4681 gccttgaccg cctgctcgag catttccagg tacatgctca agcccacttc actgagctgg
     4741 ccgctctgcg cttcgcccag gaactcgcca gcgccgcgga tttccagatc gtgtgtggcc
     4801 agcacgaatc cggcacccag atcctcgagc gattcaatgg cctccaggcg cttggccgca
     4861 tcgccggtga ggcccctacg cgacggcacg atcaggtacg cgtaggcgcg gtggtgcgaa
     4921 cgcccgaccc ggccgcgcag ctgatgcagc tgcgcgagcc ccagccggtc ggcgcgattg
     4981 atcagaatgg tgttggcgct gggaacgtcg atgccacttt cgatgatcgt ggtacacacc
     5041 agcacatcaa agcgccgatg gtagaaatcc accatcaact gctcgagttc acgctcacgc
     5101 atctgtccgt ggccgacgcg caccgcggcc tccggcacca gcgctgcgaa ctcggcggcg
     5161 accttgtcga tgctctgcac ttcgttgtgc accacataca cctggccacc acggcgcagt
     5221 tcgcgcagcg ccgcttcacg cagggtcgcg ttgtgccact cggtgatgaa ggtcttgatg
     5281 gccagacgtg ccgccggcgg tgtcgtgatc agcgacagtt cgcgcagccc ccccagcgcc
     5341 atgttgagcg tgcgcggaat gggtgtcgcc gtgagcgtca gcacatgcac ttcggcgcgc
     5401 atggtcttca gacgctcttt gtcgcgcaca ccgaatcggt gttcttcgtc aacgacgatg
     5461 agcccgaggt tctgaaagcg cgcatcggca tgcagcagcc ggtgggttgc gatcacaatg
     5521 tccaccgagc cgcgctccag accgtcgagc acggtggcgg tctcaccact tgaacgaaag
     5581 cgcgacaggg cttcgatgcg caccggccag tcggcaaagc gatcgcgaaa gctctgcgcg
     5641 tgctgctgtg ccagcaacgt cgtcggcacg agcaccgcca cctgtcttcc ggcctgtacc
     5701 gcgacgaatg cggcgcgcat cgccacttcg gtcttgccga agccgacatc gccacagacc
     5761 acacgatcca tcggtgtcgt gccctgcatg tccttgagca cctgacgaat ggcttcggcc
     5821 tgatcagcgg tttcctcaaa cgggaagcca ttggcgaaag tctgatactc gagttcgccc
     5881 gcgttgaggc tggccgctcg atgcgcagcg cgccgggcgt gcaggtccaa cagctccgcc
     5941 gcgacatcgc gtaccgcctc ggcggccctg cggcgcgcgc gcgcccactg gtcggttccg
     6001 agtttgtgca acggcgcgct ttccggtgca ccaccggtgt agcgattgac cagatgcagc
     6061 gcatgcaccg gcacatagac ccgatcgccg tcgcggtact cgagtgtcag gaactcgccg
     6121 ctctgctcgc cgatctccat gagctgcagg ccgacgtatc gcccgacacc gtaggtttcg
     6181 tgtaccactg gcgcgccggg ttcgaggctt tgcagatcgc gcaggatgac ggccggatcg
     6241 gaagcactgg tgcggcgtcg ccggcgctcc tggcgtgcgc gcgtgccgaa cagctgggat
     6301 tcacccaaca gcatcaacgc gggttgttcg agcgcgagcc cgcgcagatc ctcggccacg
     6361 cagatcgcca acgcggcgtc accctgcgtg aaggcctccc agccccgcag cgcgcgcggc
     6421 cggcggccgt gcgccccgag catttcggcg atcacctcac gccgtccggg cgaatccgct
     6481 gcgatcagca cgcgtccgac ataggtgtcc agaaacccgg tcagtggtgc cagaggctgc
     6541 gcggcgcgcg catcgagtcg aaactcgggc ggcgcggcgg tgcgaaagtc gtgtaccgcg
     6601 gcgacgtgct ccaggtcggc actcgagaag cgctcgattc cgaccgtcgg gtgtgcggcc
     6661 agcgtctcgt gcacccgtcc aggctcgacg aacacatccg gtggtggcag cagcgggcgc
     6721 tcaatatcat gacggcgatc ctcgtaacgt gccgtgatgc catcccaggc ctgctcgatc
     6781 gcctgctcca catccagatc gctggcgatc accatgtcgc gtggcagata gtcactgata
     6841 tgcgcggtcg cctcgaaaaa cagcggtaga tagaactcga tgccaggagg cgcgatgccc
     6901 tcgctgattc cccgatagat cggcatgcgc gcgacgtcgc cctcaaagcg agcgcgatat
     6961 cggcgacgga agttcttgac cgactcggca tcgagcggca tctcgcgcgc cggcaacagc
     7021 ttgagctgct ggatgctctc gagcgagcgc tgggtatcgg gatcaaagct gcggatcgaa
     7081 tcgatccggt catccagcag gtccacgcgc accggtgtgt cggtgcccat cgggtagacg
     7141 tcgaacagcg agccgcgcaa tgcgaattcg cccgggctgg tcacctgact gacgctggcg
     7201 tagccagact cgatcaggcg cgtgcgcaag ggttcgatcg ccagggcggc gccggttttc
     7261 aattcgaagc tgcgcgcctg aacgtagctg accggaggca gtcgggccag caggctgtcg
     7321 gcactgagca gcaggattcc gcgcgtgagc tgcggcaagc gcgacagcgt cagcaggcgt
     7381 tgcgagacga tatccgggtg cggcgagaac acgtcgtagg gcagcacctc ccagtccggc
     7441 agcgtcagga tcgtcagcgc atcgccacca aagaaacgca gttcgtccgc cagccgttcc
     7501 agctcgcgcg catcggcggc gatgaccacc caaggtctgc tgtccgcagc gaccgcttcg
     7561 gacaacgcca gcgagcgggc gctgccatgc agcccgcgcc agcgcgacgc ggcgtcaccg
     7621 ggcagaggca gcgggggatg tagcagggtc gttgtcgggg gcacaggggt gctagcgcag
     7681 caaagcgcgc gagcttaaac gccccggagc ggaattaaaa cttgacgggc tagggactcg
     7741 gcgccgccgg agcgcccgtg acgaccgcat gaatgacccg gtcgtgctcg aagaacaccg
     7801 agaaattcgt gtagtcccag cgggtaatcg gcggtgctcc cactgcgtcg tgacgctcga
     7861 tcggtgcgcc gaatttctct tccactttct gcatcgtcat gcccgcggag ggacgggcga
     7921 tgtcggaagg aatgacgctg acctgatcgt ccgtgacgac cagatcggct cgagccacgc
     7981 tgaccatgcc gcctgcaacg atggctgcca gtccgacagc cagcgtcagc gggaaggaag
     8041 atttctcggt catgatgcta cctgcgtaca gtcagtccga agcgaggact atatcacccg
     8101 aggccggggt gacgcctttt gtcacacatc aggcccgact tttgtgaacc aagtcctgtt
     8161 ttgagggggc ctgtgtttta atgcgctgcg catgttccag ccgctgtcag tcttcattgg
     8221 tttgcgctac gtacgctcgc gacagcacaa gtttttcgtc tcattgatca cctgggtatc
     8281 cctggcaggc gtcgggctgg gcgttgcagc cctgatcgtc atcctgtcgg tgatgaacgg
     8341 cttcgaaggg gatctgcgca gcacgctgct gtccttgagc gcccatgccc gggtgacggc
     8401 ccagggagtg cctgccgccg atgccaatgc ggcgctggca gcgcggctgc gtcgcttgcc
     8461 cggtgtggta ggcgttgcgc cctatatcga tttgaccgcc ctggtggtgc atcagaccga
     8521 aatgcagccg ataacactgc gcggggtcga cccagcgcag gagaccgcgg tggctgacgt
     8581 gcgaccgtac ctggtggagg gcgactacag cacgctcgag gcggggagca atgcggtcat
     8641 cctcggacgc gatgtcgccg atcagctcgg cgtgcgggtc ggggatgcgg tgacggtgct
     8701 agtgcccatg gtgtccggtg atcgcatgcc cgagccgcga ttgcgccagt ttacggtggg
     8761 cggggtcttc tcgtctgaac tgccggatca tgacaacacg ctggtactgg cggatatcga
     8821 tgatgtgcgc gcgttcgcgc cgcaggtcga aggtgcgagc gggctgcggc tgcgattcac
     8881 ggatgccatg caggttacgc tgtacatgcc agcggtcaga gccgccaccg gttcaggttt
     8941 cgagatcagc gattggaccc aggacaacgc cagctacttc cgcgcaatcc gcatcgaaaa
     9001 aacaatgatg tcgctgatcc tgctgctgat cgtcgcggtg gcggctttca atatcgtcgc
     9061 gatgctggtg atggtggtga acgacaagcg caccgacatt gcgattctgc gcacacttgg
     9121 ggcttcgccc ggcagcgtgc tgcgcacgtt catgacgcag ggactggtga tcgggtggtt
     9181 tggggttggg gtcggagtgc tgctgggggt attgctggca agtcatgtca cgtcgatcgt
     9241 gcccgcgctg gagagcttat tccacttcca gcttctcgac tcgaacgtct actacaacac
     9301 cgatattccc tcgcagctgc gcaccaggga tgtgttgtgg atcagcctcg cggcgctgat
     9361 tctgacgctg tgctcgaccc tctatccggc ggtgcgcgcc gcgcgcacgc cgcccgccga
     9421 tgcgctgcgc tatgaatagg ttcgcacggc gatgagctac gaatggctga tcggcacgcg
     9481 ctacgtgcgc tcgacccgtg gtcggggctt tctgtcattc atcacggtgg tgtcagcctc
     9541 ggggctggcg atcggcgtgg cggtgctgat cgtcgtgctg tcggtcatga acggcttcgg
     9601 gcgcgagctg cgtacgcgga tcctgagtgt cacctcgcac gcgacgctga cgggactgga
     9661 aggcactctg gaggactggc ggcaggatca ggccgcggcg ctgaaactgc ccggtgtgat
     9721 cgcggcggta ccctatatcg aggagcaggc actgctcgcg cacggcgcgc gcacggccgg
     9781 caccgcggtg cgtggggttg agccggagca ggaacgtcgc gccgtcggcc tcgccgagca
     9841 catccagggc ggtaggttgg aggctctgac cgacggtggc tatcggatca tcctgggcga
     9901 tgccctggcg gatgcactgt cggtgaaagt cggtgacgag gtggtattga ttgcgccgcg
     9961 gggaaatccc acaccggtcg ggatcgagcc acgcatgcgg cgttttcgcg tggccgggat
    10021 ctttcattcg ggcatgtaca agtacgatcg cggtctggcg ctgctgaatc tgcatgatgc
    10081 ggcgcgtatc tatcaactgg gcgatgcggt caccggcgtg cgcctggcgc tcagggatcc
    10141 tttcgcggcg ccacgcacgg tccatgccct ggcgctggcc ttggggggcg gcttctatgt
    10201 atccgactgg acgcgcatag agagcacgct gttccgctcg atcgagatta ccaagtcgat
    10261 gctattcgtg atcttgctgc tggtggtggc ggtggccgct ttcaatatcg tggcagcact
    10321 ggtcatggtg gtgaaggaaa agcgctccga tatcgcgatt ctgcgcacgc tcggcgccgg
    10381 gccgcgcaac gtgctggcgg cgttttccat tcagggcggc ttcatcgggc tcgccggcac
    10441 gttggccggg gcagggttgg gtctgctgtt gtcggccaat cttgaatcac tggtgcacgg
    10501 cctggagcac ctgctcggga ctcactttct ggacgccaag gtctacctga tgagcgatct
    10561 gccagggtat gcagagcccg gtgatgtgct gcgtatctgc gccgtggcgt tcgcgctgtg
    10621 tgccctggcg acgctgtacc cggcctggcg cgcggcaggc attcagccgg cggacgcgct
    10681 gcgtcatgac tgaaggcaag ccgtcgccgc cggtggtatt ggccgcacac gccgtgtgcc
    10741 gctcctttcg cgagggctcg agcacgctgg aagtgctgcg cgatgttgag ctgtcggtgg
    10801 ccgccggaga gcgcctggcg atcatcgggg tttcgggttc gggcaagacc accctgctac
    10861 aaattcttgg ggggctggat cgccctgacc gtggcagcgt gcaggtgctg gaacaggaca
    10921 ttcatgcgct gtcggagacc gagcgtggcg cgctgcgcaa tcgcgccatc ggtttcgtct
    10981 atcagtttca tcatctgtta ccggaattct ccgcactcga gaacgtcgcg atgccgctgc
    11041 tggtacgtcg cgagcgcacc ccggtggcga tcgcccgggc gcgcgacata ctgacgcgcg
    11101 tgggactggc cgagcgcctg acacaccggc cgtcgcaact gtcaggcggt gagcgccagc
    11161 gcacggcggt ggcgcgcgcg ctggtaagtt ccccatcgct ggtgctggcg gatgagccga
    11221 ccggcaatct cgatggccgc aacgccagtc aggtgttcga actgatgctg gagctaaacc
    11281 gtgagctggg caccagcctg gtggtcgtga cgcacgattc gcggctggcg gctcgcatgg
    11341 atcgggtact ggagctgagc tctggggctt tacttcagct cgcgagtccg taggcccgcg
    11401 cgcgacgtcg ttcgcggcgc acccggtact tgtgcagggt cgccatccgc cagccgattt
    11461 ccagcgcata gcgccccagc aggccgaagg tgataccgca caccaggcag cccaacagga
    11521 atggccgcca ggccggtccc agaccatgtt cgagccagtt ccaggtgagc tgaaactcga
    11581 aatgatgcgc tcgatagcgc agcagccacg cgccgatgcg gtaggccatg tagtagatcg
    11641 gtaccatcgt gaatggattg acgatgtagc aagcggcgac gcccaccgcg acgttcaggc
    11701 gcgaccacag gcagatggcg accaccagcg gcagatgcac cggcagcgga atgaagctca
    11761 cggcaatgcc gacgccaaag gcggccgtga tcgaatggcg attcagcgac cacagatgtg
    11821 aatccgtgag tcggtcaccg aagatgctca ggtaccaacg gtcccgccgc gcgtggagaa
    11881 aaccgacaaa ttgtttgact aatctacgag gcattgcatg agcactttaa cagttgcggc
    11941 cggccgggcc taagggccaa tctacgaaac tgagctttac cgcaggctct ttaggtatca
    12001 tgactatggt taagcgaatt gatgcggttt tgcaggcaac gtcacaggag ccgctctctt
    12061 aaatgtggga aatcgtgcag gcgggggggc cactcatgtg gcccatcatt ctgtgctcca
    12121 ttgtagccgc ggcgattgtc ctcgagcgca tctggaccct gcaggatcgg cgcgtcctgc
    12181 cacccgatct caccaagaaa gtctggaagc tcgtagagtc gcaccgcatc gatgacaaaa
    12241 ttgtggcagc cctcgagcag aattcgccgc tcgggaaact gctggccgtc ggactggcca
    12301 accgcgaccg tccgcgcgaa gtgctgatgg agcggctgga ggacaccggc cgtcacgttg
    12361 tgcacgaact cgagcgcttt ctaaacacgc ttggcaccat cgcgggggtc tcgccgctgc
    12421 tgggtctgct cggaaccgtg accggtatca tcaaggcctt caatgcgatc aacgccggcg
    12481 gtgcgggcga cccgcgcatg ctatccggtg gcattgccga agcactgatt gcgaccgcag
    12541 ccgggctgtg cgtggcgatt ccgtccctgg tcgcatatcg ctatctgcgc ggccgtgtcg
    12601 atcggattgt ggtggaaatg gagaagcacg cgctgcggct ggccgacgcc gtggaatccg
    12661 cttcacgccg cggcagcgag ccgcgagccg tcaacagcta gtcactcgcc atgaagatga
    12721 agccgcgccg cagcgaggag cccgagatca acgtcgtatc gctgatcgat gtcgtgttgt
    12781 tactggtggt gtttttcatg ctgtcgtcga atttcacggc cgaggggcgg ctgcagatcc
    12841 gcctgccgca ggcgaattcg gggccgacct cgcgcaacag cgacgagggg ttggtcgtca
    12901 ccgtc
//
LOCUS       DATMRM010000296         3839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_795561, whole genome shotgun sequence.
ACCESSION   DATMRM010000296 DATMRM010000000
VERSION     DATMRM010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795561"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VNZ06_08750"
     CDS             <1..744
                     /locus_tag="VNZ06_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWW20879.1"
                     /translation="IEVPALGADSAMPLGHDLDAMLTAITPQTRLVYLANPNNPTGTW
                     ADSAALQSFIERAGADTPDTLIVLDEAYLEYGRQRGTQDAIGWLAQHPNLVITRTFSK
                     AYGLAGARIGYAVSHPDVAEVLNRVRPPFNVNSLAQVAAIAALADQAYMHQVTAETMR
                     ELTRVEARIRQLGIWIAPSATNFVLARFGVPAAQVYQQLLRQGVIVRPLAAAGLPDAL
                     RISVGTPAQNERLLEALPGVLKALRAPHA"
     gene            741..2057
                     /gene="aroA"
                     /locus_tag="VNZ06_08755"
     CDS             741..2057
                     /gene="aroA"
                     /locus_tag="VNZ06_08755"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HWW20880.1"
                     /translation="MSSSLKRRYRALPSRAVRGELSVPGDKSISHRALMLGGIAQGLT
                     EISGFLASEDCLATLAALKAMGVRIERPQETQVRVYGAGRRGLSASSAPLDLGNAGTA
                     IRLLMGLLCGQRFDSTMIGDASLMRRPMERVARPLRDMGARIETTDGRPPVRIQGGAC
                     LVATDYVMPMASAQVKSAVLLAGLYATGTIRVTEPAPTRDHTERMLRDFGVALSQQGS
                     SVQMAGGQPLRGCQVQVPGDFSSAAFFIVAGCLGASDSLTLRGVGVNPTRTGLLDMLR
                     LMGAKIELRAVGSSATAEPVADITVRRSSLRGIRVPEALVPLAIDEFPIFFIAAAAAS
                     GETLVTGAGELRVKETDRLAVMAAGLATLGVRCELLPDGIRIQGGMPLRGGYVNSDGD
                     HRIAMSFAVASLLSAGAVEIDDVANVATSFPGFPTLARAAGLSLED"
     gene            2062..2742
                     /gene="cmk"
                     /locus_tag="VNZ06_08760"
     CDS             2062..2742
                     /gene="cmk"
                     /locus_tag="VNZ06_08760"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWW20881.1"
                     /translation="MSVPVVAIDGPSGSGKGTVSRMIARRTGWHLLDSGALYRLVGLA
                     GVLAGLNPDDVDGHARLARDMDVRFGADDEDRERIELNGSEVSASVRSEAAGAGASRV
                     AAWAPVREALLDRQRAFAQGPGLVADGRDMGTIVFRSAALKIFLTASAEERARRRYNQ
                     LKEKDSGVSLPALSLEIAERDRRDSTRSVAPLVPAADAIVLDSTAMTALEVVDEIMAI
                     GRARSLWT"
     gene            2900..>3839
                     /locus_tag="VNZ06_08765"
     CDS             2900..>3839
                     /locus_tag="VNZ06_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016568693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HWW20882.1"
                     /translation="MSEALSESFSEMFEQSLANQRIRPGMILTGLVVDVGSDVVIVNV
                     GLKSEAVIPLEQFKNERGEIEVAAGDQVEVALDSVEDGTGETRLSREKAKRARTWTRL
                     EEAFNNGEIVTGIISGRVKGGFTVEIDHVRAFLPGSLVDVRPVRDTGYLEGKPLEFKV
                     IKLDQKRNNVVVSRRAVVEQEFSAERTALMDNLQEGAVVRGAVKNLTDYGAFVDLGGI
                     DGLLHITDMAWKRVKHPSEVVKVGDEIEVRILKFDRERSRVSLGLKQLGADPWENIAR
                     RYPPNTRVFGKVTNIADYGAFVETEDGVEGLVHVSEM"
BASE COUNT          627 a         1190 c         1356 g          666 t
ORIGIN      
        1 atcgaggtac cggcgctcgg tgcggacagt gccatgccac tcggccacga tctggacgcg
       61 atgctcacgg cgatcacgcc gcagacgcgg ctggtgtacc tcgccaatcc gaacaatccg
      121 actggcacct gggccgacag cgcggcgctg cagagcttca tcgagcgcgc cggcgccgac
      181 acgcccgata ccctgatcgt gctggatgag gcctatctcg agtacggacg ccagcgcggc
      241 acgcaggacg cgatcggctg gctcgcgcag catccgaacc tggtgatcac gcgcacgttc
      301 tccaaagcct acggtctggc cggcgcgcgc atcggctatg cggtgtcgca cccggacgtg
      361 gccgaagtgc tcaatcgggt gcgcccgccc ttcaacgtca actcgctggc gcaggtcgcc
      421 gccatcgccg cgctggccga tcaggcctat atgcaccagg tcaccgccga gaccatgcgt
      481 gaactgacgc gcgtggaagc gcgcatccgc cagcttggca tctggattgc cccgtcagcg
      541 accaatttcg tactggcgcg tttcggtgtg cccgccgcac aggtctatca gcagctgctg
      601 cgccaagggg tgatcgtgcg cccgctcgct gccgcgggac tcccggatgc gctgcgcatc
      661 agcgtgggaa cgccggcgca gaacgagcgc ctgctcgagg cgctgcccgg ggtactcaaa
      721 gcgctgcgcg cgccgcacgc gtgagcagca gcctcaaacg ccgctatcgg gcgctgccct
      781 cgcgggctgt gcgcggcgag ctttcggtgc cgggggacaa gtcgatttcg catcgcgcgc
      841 tgatgctcgg cgggatcgcg caagggctca ccgagatctc gggttttctg gcgagcgagg
      901 attgtctggc caccctggcc gcgctcaagg cgatgggtgt tcgcatcgag cgcccgcagg
      961 aaacccaggt gcgcgtctat ggcgcgggca ggcgcggact gagcgccagc tcagcgccgc
     1021 tggatctggg caatgcgggc acggcgatcc gtctgctgat gggactgctg tgcggtcagc
     1081 gcttcgacag cacgatgatc ggtgatgcgt cgctgatgcg ccggccgatg gagcgcgtcg
     1141 cgcggccgct gcgcgatatg ggcgcgcgca tcgaaaccac cgatggcagg ccgccggtca
     1201 ggattcaggg cggcgcctgc ctggtggcga cggattatgt catgcccatg gccagtgccc
     1261 aggtgaagtc cgcggtgctg ctggccgggc tgtatgcgac cggcacgatc cgtgtcaccg
     1321 agccggcacc gacccgcgat cacaccgagc gcatgctgcg cgacttcggc gtcgccctgt
     1381 cgcagcaggg atccagcgtg cagatggccg gaggccagcc gctgcgcggc tgtcaggtac
     1441 aggtgccggg ggatttttcc tccgcggcgt tttttatcgt ggccggatgt ctgggcgcgt
     1501 ccgactcgct gacgctgcgt ggggtcggcg tcaatccaac gcgcaccggt ctgctggaca
     1561 tgctgcgact gatgggcgcg aagatcgagc tgcgcgcggt cggcagctct gcgaccgcgg
     1621 agcccgtcgc cgatatcacc gttcgacgca gcagcctgcg cggcatccgc gtgcccgagg
     1681 ccctggtgcc gctggcgatc gatgagtttc cgattttttt catcgccgcg gcggctgcca
     1741 gtggtgagac gctggtgacc ggcgcgggcg aactgcgggt caaggagact gaccggctgg
     1801 cggtgatggc tgcggggctt gcgacgctgg gtgtacgctg cgagctgctg ccggatggca
     1861 ttcggatcca gggaggaatg ccgctgcgcg gaggttatgt gaacagcgac ggggatcacc
     1921 ggatcgcgat gtcctttgcc gtggcaagcc tgctcagcgc cggagccgtg gagatcgatg
     1981 acgtggcgaa tgtcgcgacc tcatttccgg ggtttccgac gctggcccgg gccgccgggc
     2041 tctcactcga ggactgaggc cgtgtcggtg cccgtggtgg cgatcgacgg accgagcggc
     2101 tcgggcaagg ggaccgtcag ccgcatgatc gcccgccgta ccggctggca cctgctcgac
     2161 agtggcgctt tgtaccgcct ggtcggccta gccggggtgc tggccgggct caaccccgac
     2221 gatgtagacg gtcacgcccg cctggcacgc gatatggacg tgcggttcgg cgctgacgat
     2281 gaggaccggg agcgcattga gctcaacggc tccgaggtga gcgcctcggt acgctccgag
     2341 gcggccggtg ccggggcttc ccgggtcgcg gcctgggcac cggtacgtga ggcgctgctg
     2401 gatcgtcagc gcgccttcgc ccagggtccg ggtctggtgg cggatgggcg ggacatgggc
     2461 accatcgttt tccggtctgc cgccctcaaa atcttcctca cggcaagcgc tgaagagcgc
     2521 gcccgcagac gctataacca gttgaaagaa aaggattctg gtgttagcct tcccgccctt
     2581 tcgctcgaga tcgcggaacg cgaccgacgc gattcgacac ggtccgtagc gccgcttgtg
     2641 ccggctgccg acgcgatcgt cctggactcg accgccatga cggccctcga ggtcgtggac
     2701 gagatcatgg cgatcggccg cgctcgctcg ctctggacct gaatcgcgct cgtcgcccgg
     2761 tgccatggcg cgccggtccg tgtgactcga gcgttaagag acgtcgatgc cgcagggacg
     2821 cggtgtcttg aaaatgtaac ccgcatggcc ggaggccagg gcggccgagc aaaaagcacg
     2881 gcgacgtgcg agagagtgta tgagcgaagc ccttagcgaa agtttcagcg agatgttcga
     2941 acagagcctg gcgaatcagc gcattcgtcc gggcatgatt ctgaccggtc tggtggtgga
     3001 tgtgggttcc gacgtcgtga tcgtcaacgt cggactgaaa tccgaagcgg tgattccgct
     3061 cgagcaattc aagaatgaac gcggcgagat cgaagtcgcg gccggcgatc aggttgaagt
     3121 ggcactggac tcggtggagg atggcacggg tgagacccgg ctgtcgcgcg agaaggccaa
     3181 gcgcgcccgt acctggacgc ggctggagga ggccttcaac aatggtgaga tcgtcaccgg
     3241 catcatttca ggacgggtga agggtggctt caccgtcgag atcgatcatg tgcgtgcatt
     3301 tcttcccggt tcactggtcg acgtgcggcc ggttcgcgat accggctatc tggaaggcaa
     3361 gccgctcgaa ttcaaagtca tcaagctcga tcagaagcgc aacaacgtcg ttgtttcgcg
     3421 tcgtgcggtg gtggagcagg aattctccgc cgagcgcacc gcgctgatgg acaacctgca
     3481 ggaaggcgcg gtggtgcgtg gtgcggtcaa gaacctcacc gactacggtg cgttcgtgga
     3541 cctgggtggc atcgacggtc tgctgcacat caccgacatg gcatggaagc gcgtcaagca
     3601 tccgtcggaa gtcgtcaagg tcggcgatga gatcgaagtg cgcatcctga agttcgatcg
     3661 cgagcgctca cgcgtgtcgc tcggactcaa gcagctcggc gcggatccgt gggagaacat
     3721 cgcccgccgg tatcctccga acacgcgtgt gttcggcaag gtcaccaaca tcgccgacta
     3781 cggtgcgttc gtcgagaccg aggacggcgt cgaaggtctg gtgcacgtct cggagatgg
//
LOCUS       DATMRM010000297         5107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3886901, whole genome shotgun sequence.
ACCESSION   DATMRM010000297 DATMRM010000000
VERSION     DATMRM010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3886901"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VNZ06_08770"
     CDS             complement(<1..461)
                     /locus_tag="VNZ06_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20883.1"
                     /translation="MSAHWVAWQVLTRSQLREQPLRVLATIVAIALGVALGSAVYLIN
                     TAALGEFDLATRRLIGSADLVVRGSPQGFDESLFVQLVRDPQVALASPVLELELTLPG
                     DNAPLKVLGVDSFRASALQPQLMGELGGNATQLFAPDSIVLTRAAAQQLQLV"
     gene            complement(458..1123)
                     /locus_tag="VNZ06_08775"
     CDS             complement(458..1123)
                     /locus_tag="VNZ06_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWW20884.1"
                     /translation="MLRLQAISKRFRAEGAPVLDRLELTLEPGEYLAIMGESGAGKST
                     LLNVIAGLDRPDSGSVLFDGTELAALDDDAITRWRRAHMGFVFQAFHVLPYLRVEQNV
                     ALPLELLDVSDPQRREAVRQMLAACGIEALSERYPRELSGGELQRIAIARALVHRPQL
                     LLADEPTGNLDARTADQILRLLRAQLQATHAGAILITHSMTAARSADRILKLEGGQLQ
                     PLA"
     gene            complement(1123..1917)
                     /locus_tag="VNZ06_08780"
     CDS             complement(1123..1917)
                     /locus_tag="VNZ06_08780"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HWW20885.1"
                     /translation="MWPEFIHLWLIGLLFAFVLMAAIWGIALRIGNAGIVDIAWSAGF
                     APMAIFFALAGDGLWLRRAVIALLVSLWSLRLGGYLYVRVMSHHPKEDRRYAELRKAW
                     GSSTHRNLFWFFEMQALLLAILSAPFLLICLNAAPDMKPLEFAGILVFVIALSGESLA
                     DRQLARFRGDPANQGKVCDVGLWRLSRHPNYFFEWMVWVAYLILACASPFGWIALYCP
                     ALMLFFLLRVTGIPMTERLSLQSKGQAFADYQRRTSPFVPWFPKRS"
     gene            complement(1907..2398)
                     /locus_tag="VNZ06_08785"
     CDS             complement(1907..2398)
                     /locus_tag="VNZ06_08785"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HWW20886.1"
                     /translation="MLREAWQRRVVDVVLAQLRQGITPQKIALTVAIGATLGLFPILG
                     STTLLCFLAGLLLKLNQPIIQAINYLVYPLQVTGIYFFIRVGEWLTRTPPLQFSIAGL
                     LQQFRVAPLHFFQEFGMTALRGVLAWTLIAPFVAAALYFGLLPVLRRLARLRSPGVNV
                     NVA"
     gene            complement(2388..2726)
                     /locus_tag="VNZ06_08790"
     CDS             complement(2388..2726)
                     /locus_tag="VNZ06_08790"
                     /inference="COORDINATES: protein motif:HMM:NF018991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1475 family protein"
                     /protein_id="HWW20887.1"
                     /translation="MIWFLRFAFLGVLITMLAVTGWASSLLPFWHTPRSLVLHPWFIA
                     TLFDTYFGFLTFWLWLAYRETLWVARIVWLIAIVLLGNIAMSFYMLLIVWRLPPGAGM
                     RDVLLRPHAA"
     gene            2855..4300
                     /locus_tag="VNZ06_08795"
     CDS             2855..4300
                     /locus_tag="VNZ06_08795"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HWW20888.1"
                     /translation="MRSPTRTIAIIGAGFSGTVLAVNLLRLVSAQPLRIVVIESRAQV
                     GRGVAYQDDGHPHLLNVPAARMSAQSRDPTQFARYARARDASYRADQFLPRRLYGEYL
                     QALLQNAAQSAPAHVRLERIDARAEQIFCIDPRGPQLVILSNQQHLVADDVVLACGDP
                     PPAAPASAAAITGHGAYVGDPFSRGALGPEAETVLLLGSGLTMADVTLATASLNPSVR
                     IHAISRHGLLPQAQTSAPAASVTGDLLSYLPPGVITARALLRAFRALLTDVERQQGDW
                     RDAVNLARQGAAVAWQRLSLPQRARFLRHLRSRWDVHRHRMPPESDVRLKALRQSGQL
                     QLHAGYLLSMSEEAERIRVHWRERGSQSTQSLLVDRVINCTGTDRRLTQTPHRLLQAL
                     MAEGLATPDPLGLGLRTAEHGALIDREGRAAQHLFYVGPMLRAQHWEATAVAELRAHA
                     EGLAQALLRISVSQSAPLVAPAGRLDQLAFK"
     gene            complement(4241..4522)
                     /locus_tag="VNZ06_08800"
     CDS             complement(4241..4522)
                     /locus_tag="VNZ06_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HWW20889.1"
                     /translation="MLQRIAVGLSVGSLTAFRSTAHAADPLLNEQDPEARKVQYVEDA
                     SRAKEAQASGQKCSNCSVYTAMGDTQGSCGLFKGKLVKAAGWCNEWSGL"
     gene            complement(4552..>5107)
                     /locus_tag="VNZ06_08805"
     CDS             complement(4552..>5107)
                     /locus_tag="VNZ06_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWW20890.1"
                     /translation="LLVHGGSSGIGLAAVQLARHVGAEVLITAGSEQKCEACRSFGAA
                     LAVNYRTEDFVARVRDYTGGKGVDVVLDMVGGDYIPKNLGLLRDSGRLVFVAFLHGSR
                     VEVDFTPIMLKRLRVTGSTLRPRTVAEKAAICAALRREIWPDYTAGKLRSHIHASFPL
                     ERVAEAHRLMESSQHIGKIVLQAS"
BASE COUNT          970 a         1689 c         1616 g          832 t
ORIGIN      
        1 acgagctgca gctgctgcgc ggccgcgcgt gtcagcacga tgctgtccgg cgcgaacagc
       61 tgtgtggcat tgccacccag ttcacccatc agctgcggct gcagcgccga ggcgcgaaac
      121 gaatcgacac cgagcacctt cagtggcgca ttgtcacccg gcagggtcag ctcgagttcc
      181 agtacggggc tggcgagcgc gacctgcggg tcgcgcacca gttgcacgaa cagcgactcg
      241 tcgaatccct gtggcgaacc gcgcaccacc aggtccgcgc tgccaatcag tcgccgcgtg
      301 gccagatcga actcccccag ggccgcggtg ttgatcagat agaccgcact gcccagggca
      361 accccgagcg cgatcgcaac gatggtggcc agcacgcgca gcggctgctc gcgcagttgc
      421 gaacgcgtga gcacctgcca ggcgacccag tgcgcgctca tgcgaggggc tgcaactgcc
      481 cgccctccag tttcaagatg cggtcggcac tgcgcgccgc agtcatcgag tgcgtgatca
      541 ggatcgcgcc ggcatgcgtc gcctgcagct gtgcgcgcag cagccgcagg atctgatcgg
      601 cagtgcgcgc atccagattg ccggtcggct catccgccag aagcagctgc ggtcggtgca
      661 ccagcgcgcg cgcaatggcg atgcgctgca gctcaccgcc cgacagttcg cgcggatagc
      721 gttccgacaa cgcttcgatg ccgcacgccg cgagcatctg gcgcaccgcc tcgcggcgct
      781 gcgggtcaga cacgtccagc agttcgagcg gcagcgcgac gttctgctcg acgcgcaggt
      841 agggcagcac gtgaaacgcc tgaaacacga atcccatgtg cgcccgtcgc cagcgcgtga
      901 tcgcatcatc atccagcgcc gcaagctcgg tgccatcaaa caacacgctg cccgagtccg
      961 ggcggtcaag gcctgcaatc acgttcaaca gtgtcgattt gccggcgccg gactcaccca
     1021 tgatcgccag gtattcaccc ggctcgaggg tgagttcaag cctgtccagc accggcgcgc
     1081 cttcggcgcg gaagcgtttg ctgatcgctt gcagtcgcag catcagctgc gcttggggaa
     1141 ccagggaacg aaggggctgg ttcgccgctg atagtcagcg aaggcctggc ccttgctctg
     1201 gagcgagagt cgttcggtca tcgggattcc ggtcacgcgc agcaggaaga acagcatcag
     1261 cgcggggcag tagagcgcga tccagccgaa cggggatgcg caggccagta tcagataggc
     1321 aacccacacc atccactcga agaagtagtt cgggtggcgc gacaggcgcc ataggccgac
     1381 gtcacagacc ttgccctggt tggcggggtc acccctgaag cgcgccagct gccggtcggc
     1441 cagcgattca ccggacagtg caatcacgaa caccaggatg ccggcaaact ccaatggttt
     1501 catgtccggc gccgcattca ggcagatcag caggaagggc gccgacaaaa tcgcgagcaa
     1561 cagcgcctgc atctcgaaga accagaacag gttgcggtgg gtgctcgagc cccaggcttt
     1621 acgcagctcg gcgtagcgtc gatcttcctt gggatggtgc gacatcacgc gtacatagag
     1681 atagccgccc agtcgcagac tccacaggct caccagcagc gcgatcactg cccgccgcag
     1741 ccagaggcca tcgcctgcga gtgcgaagaa gatcgccatc ggcgcgaagc cggccgacca
     1801 ggcgatatcg acgataccgg cattgccgat gcgcagcgcg atgccccaga tcgcggccat
     1861 cagcacgaat gcgaacagca gtccgatcag ccatagatga atgaactcag gccacattga
     1921 cgttgactcc ggggctgcga agcctggcca ggcggcgcag caccggcagc aggccgaagt
     1981 acagcgcagc ggcaacgaac ggcgcaatca gggtccaggc cagcacgccg cgtagcgccg
     2041 tcatcccgaa ctcctgaaag aagtgcagcg gtgcgacgcg aaactgctgc agcaggcccg
     2101 caatcgagaa ctgcagcggc ggtgtgcggg taagccactc gccgacgcgg atgaagaaat
     2161 aaattcccgt gacctgcagc ggatagacca ggtagttgat cgcctggatg atcggctggt
     2221 tgagtttcag cagcaggccg gccaggaagc acagcagcgt ggtgctgccc aggatcggaa
     2281 acaatccgag cgtcgcgccg atcgccacgg tcagtgcgat cttctgtgga gtgattccct
     2341 ggcgcagctg cgccagtacc acgtccacga cgcgtcgttg ccaggcctca cgcagcatgg
     2401 ggacgcagca gcacgtcacg catgccggca ccgggtggca ggcgccacac gatgagcagc
     2461 atatagaacg acatcgcgat attgcccagc agcacgatcg cgatcagcca cacgatgcgt
     2521 gcaacccaga gcgtctcgcg ataggccagc cacagccaga acgtcagaaa cccaaaatag
     2581 gtatcgaaca gcgtggcgat gaaccacgga tgcagcacca gcgatcgcgg tgtatgccag
     2641 aagggcagga ggctgcttgc ccagcccgtc accgccagca tcgtgatcag caccccgagg
     2701 aaggcgaatc gcagaaacca gatcatgagg agaccttata tatcttaaaa tatattttga
     2761 gatagatctt aacgaaatct ggcatttacc gctcaacctg attgtcaccc gcgttcgact
     2821 gacttgatac cgcaggcccg cctatcgtgc cgccatgcgc tcacccactc gcaccattgc
     2881 gatcatcggt gctggtttca gcggtacggt actcgctgtc aatctgcttc gcctcgtatc
     2941 cgcgcaacct ctgcgcatcg tagtgatcga gtcgcgtgcg caggtaggcc ggggtgtggc
     3001 gtaccaggac gatggccatc cgcatctgct gaatgtgccg gccgcgcgca tgtcggcgca
     3061 gtcgcgcgac ccgacgcagt tcgcacgcta tgcgcgggcg cgcgatgcga gctaccgagc
     3121 cgatcagttc ttaccgcgcc ggctgtacgg ggagtatctg caggcattgc tgcagaacgc
     3181 cgcgcagtca gcgcctgcgc acgtcagact cgagcggatt gacgcccggg ctgagcagat
     3241 attttgcatc gacccccgag gaccgcagct tgtcattctc tcgaaccagc agcacctggt
     3301 tgccgacgat gtcgtattgg cctgcggcga cccgcctccc gccgctccgg ccagcgcagc
     3361 agccatcacg ggccatggcg cttacgtggg cgacccattc agccgcggcg cgcttgggcc
     3421 ggaggcggaa acggtgctgc tgctcggttc aggtctcacg atggccgatg tcacgctcgc
     3481 gaccgcctcg ctgaacccgt cggtacgcat tcacgcgatc tctcgtcacg gactgctgcc
     3541 gcaggcgcag acctcagccc cggcagcctc ggtcacgggc gatctgctgt cgtatcttcc
     3601 gcccggtgtg atcaccgccc gtgcgctgct gcgcgcgttt cgtgccctgt tgaccgacgt
     3661 tgagaggcag cagggcgact ggcgcgacgc ggtgaacctc gcacgccagg gcgccgctgt
     3721 ggcgtggcag cggctgagtc tgccgcaacg cgcgcgtttc ctgcggcacc tgcgtagccg
     3781 ttgggacgtg caccgccatc gcatgccgcc ggagagcgat gtgcgcctga aggcgctgcg
     3841 ccagtcgggt cagttgcagc tgcatgccgg atacctgtta tcgatgagcg aagaagctga
     3901 gcgtatccgc gtgcattggc gtgagcgtgg atcgcagtca acccagtcgc tgctggttga
     3961 ccgggtgatc aattgcaccg ggaccgatcg gcggctgaca cagacacccc atcggttgct
     4021 gcaggccctg atggccgagg gactggcgac gccagaccct ttgggactgg gcttgcggac
     4081 cgctgagcac ggcgcgctga tcgatcgcga gggccgcgcc gcgcagcatc tgttctatgt
     4141 ggggccgatg ctgcgggcgc agcactggga agccaccgcc gtggcggaac tgcgcgcgca
     4201 tgcggaaggg ctggcgcaag ccctgctgcg catcagcgtc tcacagtccg ctccactcgt
     4261 tgcaccagcc ggccgccttg accagcttgc ctttaaatag gccgcagctg ccctgagtgt
     4321 ctcccatcgc cgtgtagacg gagcaattcg agcatttctg gccggacgcc tgcgcttcct
     4381 tcgctcgact cgcgtcttcg acgtactgca ccttcctggc ctccggatcc tgctcgttca
     4441 gcagcggatc ggcagcgtgc gcggttgatc tgaacgctgt cagcgagccc accgacagcc
     4501 cgaccgcgat ccgttgcagc atcgcgcggc gggaaagagg aaagggtcgg gtcatgaggc
     4561 ctgcagaaca atcttgccga tgtgctgact gctttccatc agccgatgcg cctcagcgac
     4621 gcgctcgagc ggaaagctcg cgtggatatg cgacctcagc ttgccggcag tatagtccgg
     4681 ccagatctcg cgtctcaacg cggcgcagat cgccgccttc tccgcgaccg tgcgtggacg
     4741 cagcgtcgag cccgtgacac gcagacgctt gagcatgatc ggcgtgaagt ccacctcgac
     4801 gcggctgccg tgcaggaaag cgacgaacac cagccggccg ctgtcgcgca ataatccaag
     4861 attcttcggg atgtaatcgc ctccgaccat atcgagcacc acgtccacac ccttgccgcc
     4921 ggtgtagtcg cgcacgcgcg ccacgaaatc ctcggtgcgg tagttgactg ccagcgccgc
     4981 gccgaaactg cggcaggcct cgcatttctg ctcgctgccg gcggtgatca gcacctcggc
     5041 accgacatgc ctggcgagct gcacggccgc cagtccgatg ccgctcgagc cgccgtgcac
     5101 cagcagc
//
LOCUS       DATMRM010000298         3383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4526991, whole genome shotgun sequence.
ACCESSION   DATMRM010000298 DATMRM010000000
VERSION     DATMRM010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4526991"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /gene="dapE"
                     /locus_tag="VNZ06_08810"
     CDS             <1..905
                     /gene="dapE"
                     /locus_tag="VNZ06_08810"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF009557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HWW20891.1"
                     /translation="EEWTSDPFKPVVRDGLLYGRGAADMKTGLAAMVTATERFVAEHP
                     DHRGTVAFLITSDEEGPSVDGTKRVVETLRERGERIDYCLVGEPSSEQAVGDTIKIGR
                     RGSLSGRLTVHGVQGHIAYPQLAENPVHTFAPALAELAGRVWDQGNEHFQPTTFQVSN
                     LNAGTGAPNVIPGELKARFNLRYSPVQTLDGLRRTVEEVLKRHRVHYTIEWYVSGEPF
                     YTPPGPFSNAVCDAVRALTGRQPKLSTGGGTSDGRFIAPMGAQVVELGVVNASIHKVN
                     ECVRVEDIDTLHALYLQTLKNLLS"
     gene            complement(898..1389)
                     /locus_tag="VNZ06_08815"
     CDS             complement(898..1389)
                     /locus_tag="VNZ06_08815"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HWW20892.1"
                     /translation="MRRGLNALGLAACAGLLGFAYYAQFSLGLEPCPLCIFQRVGVFA
                     IGVIFLLAALHNPRSWGARVYGMLLLIAALITMGVSARHLWIQHLPEGAVPACGATLS
                     YMMQIFALSDVIRKVLTGSGECARITWSFLGLSMPGWVLLAAAGLGAYGLWVNRPAKR
                     AVS"
     gene            complement(1389..2135)
                     /gene="phoU"
                     /locus_tag="VNZ06_08820"
     CDS             complement(1389..2135)
                     /gene="phoU"
                     /locus_tag="VNZ06_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019694308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWW20893.1"
                     /translation="MTQATMEAADISHHTSRRYNQDLERVRSKVLAMGGFVEDQLSRS
                     LMALIDADSSLGRAVASDDYMVNGMELSIDEECSRILATRAPAAGDLRMIVATIKAIT
                     DLERIGDECEKVGLIAARLATAERPADRYREVKHLGRRVQMLVHDTLDAFARLDATAA
                     LETCRKDRAIDEEYEAIQRRCIADMTGDSSVIRRTLDIMWVVRSLERIGDHAKNICEY
                     VIYMVHGKDVRHTSLDHVERELAESAIRRG"
     gene            complement(2231..>3383)
                     /gene="phoR"
                     /locus_tag="VNZ06_08825"
     CDS             complement(2231..>3383)
                     /gene="phoR"
                     /locus_tag="VNZ06_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HWW20894.1"
                     /translation="WLRADQMALAPDLAGPWGELVAMVARLYRRKQFHKQRLLRLLRE
                     LRRSTAAMPDGVVVLNPQAEILWFNRTSARLLGFRGKSDFGLRIENLVRQPEFVRYLR
                     DGNYSGPVIVRSAVGGDQYLSLQIVPYGNGQLLTLVRDVTREYRLEAMRKDFVANASH
                     ELCSPLTVISGYLETLTQDSDIDPTLAGPLQEMRRQALRMTSIVQDLLELSRLESSDS
                     DAPRDPIDVAALMAQLRQDVMARSPHPTLVQVKADSSARLLGEEIQVHSAFSNLVDNA
                     AKYTPPEGSVQIRWWTDEKGGHFSVTDTGIGIAASHIPRLTERFYRVDPGRSRATGGS
                     GLGLAIVKHVLQRHGADLSIESEEGRGSKFTCHFPRERLQNVPVLAAVN"
BASE COUNT          670 a         1113 c         1015 g          585 t
ORIGIN      
        1 tggaagagtg gacctccgac ccattcaagc cggtcgtgcg cgatgggctg ctgtatggac
       61 gcggcgcggc cgacatgaag acggggctgg cggccatggt gacggcaact gagcgcttcg
      121 tggccgagca ccccgatcac cgcggtaccg tggccttcct gatcaccagc gacgaggaag
      181 gtccgtccgt agacggtacc aagcgagtgg tcgaaaccct gcgggaacgc ggcgagcgca
      241 tcgactattg cctggtgggc gaaccctcga gcgagcaggc ggtcggcgat accatcaaga
      301 tcggacgacg aggctcactg tcggggcgcc tgacggtgca cggggttcag gggcacatcg
      361 cttacccaca gttggcggaa aatccggtcc acaccttcgc gcccgcgctg gccgagttgg
      421 ccggacgggt ctgggatcag ggcaacgagc attttcagcc gacgaccttc caggtctcga
      481 acctcaacgc cggcaccggc gcgcccaacg tgatcccagg cgaactcaaa gcgcgcttca
      541 atcttcgcta ctcgccggtt cagacgctgg acgggctccg gcgcaccgtc gaggaagtgc
      601 tgaaacgaca ccgggtccat tacacgatcg agtggtacgt ctccggtgaa ccgttctata
      661 caccaccggg ccccttttcc aacgcggtct gcgatgcggt gcgtgcgctg accggccgcc
      721 agccgaaact ctccaccggc ggtggcactt ccgacggccg gttcatcgcc cccatgggcg
      781 cgcaggtggt ggagctcggg gtcgtgaatg ccagcattca caaggtcaat gaatgcgtgc
      841 gggtcgagga tatcgacacg ctgcacgcgc tctatctgca gacgctgaaa aacctgctca
      901 gctgactgcg cgcttcgcgg gccggttcac ccacagcccg taagcaccta agcctgccgc
      961 agccagcaac acccagccgg gcatcgatag ccccagaaaa gaccaggtga tccgggcgca
     1021 ctcgccggag ccggtgagca ctttgcggat cacgtcggac agcgcaaaga tctgcatcat
     1081 gtaagagagt gtcgcgccgc acgccggcac agcgccttcg ggcaggtgct ggatccacag
     1141 gtgccgcgcc gagaccccca tcgtgatcag cgctgcgatc agcaacagca ttccatagac
     1201 gcgcgcaccc cagctgcgcg gattgtgaag cgcggcaagt aagaaaatca cgccgatagc
     1261 gaacacgccg acgcgctgaa agatgcacag cggacagggt tcgaggccga gggaaaactg
     1321 ggcgtagtag gcgaaaccga gtagccccgc gcaagccgcc aggcccagag cgttcagtcc
     1381 acggcgcatc agccgcgacg gatcgccgac tcggccagct cacgctcgac gtgatcgagc
     1441 gaggtgtggc gcacgtcctt accatgcacc atgtagatca cgtactcaca gatgttcttg
     1501 gcatgatcgc cgatgcgctc gagggatctc acgacccaca tgatatccag cgtgcgacgg
     1561 atgacactgg aatcgccggt catgtcagcg atgcagcggc gctgaatcgc ttcgtactct
     1621 tcgtcgatcg ctcgatcctt gcggcaggtc tccagcgccg ctgtagcgtc cagccgtgcg
     1681 aacgcgtcga gcgtatcgtg taccagcatc tggacgcgcc gacccagatg cttgacctcg
     1741 cgataccgat cggccggccg ttccgcggtt gccagccgtg ccgcgatcaa ccctaccttc
     1801 tcgcactcat cgccgatgcg ttccagatcg gtaatagcct tgatggtggc gacaatcatc
     1861 cgcagatccc cggccgcagg agcacgcgtc gccagaatgc ggctgcactc ctcgtcgatc
     1921 gacagctcca tgccgttgac catgtagtca tcgctggcga ccgcccgacc caatgaactg
     1981 tcggcatcga tcaatgccat cagactgcgg ctgagctgat cctcgacgaa cccgcccatc
     2041 gccagtacct tgcttcgaac ccgctccagg tcctggttgt accgccgaga ggtatggtgg
     2101 gagatgtcag cagcttccat ggtggcctga gtcataactc atcgcgaaaa tacgcgaact
     2161 gtccagaata tagcgcaatc gttggccgac gcacgcgaaa tagatcgcac ctttgtgcgc
     2221 tgtcgcacac ttagttcacc gccgcgagca ccggtacgtt ctgcagcctt tcccgcggga
     2281 aatgacaggt gaatttgctg ccccgccctt cttcgctttc tatcgacaga tccgcaccat
     2341 gccgttgcaa tacgtgcttc acgatggcga gccccaggcc cgagccgccg gttgcacggc
     2401 tgcgacctgg atcgacccga tagaaacgct ccgtcaggcg cggaatgtgg ctggcggcaa
     2461 taccgatgcc ggtatcggtc acagagaaat gccccccttt ctcatccgtc caccagcgta
     2521 tctgcaccga accttcgggc ggcgtgtact tggcggcgtt gtctaccagg ttggagaagg
     2581 ccgagtggac ctgtatctcc tcacccagca agcgcgcgct cgaatcggct ttcacctgta
     2641 ccagcgttgg atgcgggctg cgcgccatga catcctggcg cagctgcgcc atcaaggctg
     2701 ccacgtcgat cggatcacgc ggcgcgtcac tgtctgagga ttccagacgt gaaagctcca
     2761 gcagatcctg cacgatgctg gtcatccgca gagcctgccg gcgcatctcc tgcagcgggc
     2821 cggccagcgt cgggtcgatg tccgagtcct gggtcagtgt ctccagatag cccgagatca
     2881 ccgtgagcgg ggaacacaac tcgtgcgacg cgttggcgac gaagtccttg cgcatcgctt
     2941 ccagccgata ctctcgggtc acatcgcgca ccagcgtcag caattgtcca ttgccataag
     3001 gcacgatctg cagcgacaga tactgatcac cgccgaccgc cgaacgcacg atcaccggtc
     3061 cggagtagtt gccatccctc aggtaacgca cgaactcggg ctggcgcacc aggttctcga
     3121 tgcgcaggcc gaaatcgctt ttgccgcgaa atcccagcag ccgagccgag gtccggttga
     3181 accacaggat ttcggcctgc ggattgagta ccacgacgcc gtcgggcatc gcagcggtag
     3241 agcgtcgcag ctcgcgcagc agccgcaaca agcgctgctt gtggaattgt ttgcgtcgat
     3301 agagacgcgc caccatcgca accagctcac cccagggccc agccagatcc ggtgccagcg
     3361 ccatctgatc ggcccgcagc cag
//
LOCUS       DATMRM010000299         9099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3250037, whole genome shotgun sequence.
ACCESSION   DATMRM010000299 DATMRM010000000
VERSION     DATMRM010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9099
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3250037"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VNZ06_08830"
     CDS             complement(<1..337)
                     /locus_tag="VNZ06_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20895.1"
                     /translation="MSGWWPNQVPLRLRVFFRSAFLLLALATVALALFELREEKQLSY
                     RAYRELFDRNTRQIGARLQHPSGQLALLNPSLPGATLTPLRPLLLPFSAIDFDDRTKA
                     QQAAEMAGCL"
     gene            complement(334..1023)
                     /locus_tag="VNZ06_08835"
     CDS             complement(334..1023)
                     /locus_tag="VNZ06_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW20896.1"
                     /translation="MAVIEDDVPTSNQIKGWIESARSGIRVDQWYTRDEATSAVDREP
                     YDLVVLDIELGRERHAGVAVINAINKRRKIPVLVVSGLPAGIYRSIMKALDAWDYLQK
                     SDFDENEFVETFLDMLRSMQDPSLPLIASVSNATHQLSLDPLRQNSPLWRGQRINLPL
                     TSQRILDALYQKRGRIVSYDELFQVVKSGRNRDNVRKHISTIREAFRELDPRFDLIEN
                     VPMRGFRWVDA"
     gene            1208..2398
                     /locus_tag="VNZ06_08840"
     CDS             1208..2398
                     /locus_tag="VNZ06_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWW20897.1"
                     /translation="MTDRFTAILELLRAMGASISAAMATVGAGLGRLRVQLLVLAALV
                     LIAWMIVRHPPFASVQRGEIAVRTNLLTGQVSQFREGSLWVIPGLQQVRYFPTRDQIY
                     RPAQSAKANGEAPFQSVEGLSLGVDLTVRYAIDPARMASIAKTLPDELDDVIEPAVKG
                     VVYPLFAGYTVKDIFTTRRAEIQKAIEEQLRPKLAADGFLLRSVQMGEVDLPRDYRLG
                     MEKLLAEELESEKMRYTLELKDKEVKARALEGEADKVRRQKAAEAAGEEQVIAAKAQE
                     EAMKHVLPFKQKQIEQRRLEAEAEKVARIRSAEGTADARRIEASGEADSRQKLADAEV
                     YRLEKINRATSEQMQREGALISQNPLLIQKTMADKLSDKVQVIIAAPPAEGGFIGSNL
                     LGAK"
     gene            2395..3807
                     /locus_tag="VNZ06_08845"
     CDS             2395..3807
                     /locus_tag="VNZ06_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20898.1"
                     /translation="MSALLASAMLAIVTQNQVALRAAPRDSAPQQAMLWQGEALDVRD
                     ARGDYLLVYDLKLEREGYIRSNQVRLTTLRPEEAPELLAVVRFLRDTPGSEALGISYA
                     AAYLKAIPASAMTAEPLDALGNMAQRLAQRASSVQSKSAQATISAHLEVVSQMGVTMT
                     GFERDGSVQICYDGEVFRKVLAMSSADPAELARAALGLTRHECVDPNMGPIERYQFDQ
                     WRADVLDRVPTDELILGLKSRVLMRRAGVLASLAFWQARRGLNPQLAAERSIEDLAGV
                     DKSVLDDEGLRDYAEAAVRVGASRLAALKMANRGGKLVVHTSAGAPGETCVSLFYMQK
                     QDGEPLIRRCTYGTVWSSSMSSNVEGTALTLAVQPLATWRELWVFRKTADGWTANVLP
                     PDLNGPGLGYIESAGWDPGTKRLLLCREALVSDHLLRRFELVRLDTLATVHQASTPEY
                     LTNFARWQDPVWRSNTVALR"
     gene            complement(3982..5742)
                     /locus_tag="VNZ06_08850"
     CDS             complement(3982..5742)
                     /locus_tag="VNZ06_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWW20899.1"
                     /translation="MKTLGRILLAALLLVGVTAGIGLLIMPTPLTLLGAVTNTVRTLS
                     NPPGTLSVEVRPGSPAPELGAAPTDTPDPNEWPSYNRTLTSQRFSPLDQINRQNIKSL
                     RVLCVYDSGIHEGFESGPIMVDGALIATGAFDTFSIDPSSCRQNWRVHENYTPRSPVL
                     ASRGAAYLQGRVFRGTLDGRVLAYDSHTGHRLWQASVADPKLGTNICSAPIAWDGMVF
                     AGICGGDSKGIKGRMYGIAADSGRIVWETYLVPKQAGDPSFGPAGRIPDGALQSWDNR
                     DTPGLPISGGGTWTSYTLDPATGWLYIPVGNPSPDFVDAARAGSDLYVDSVLILDAKT
                     GDYVNHFQMEPRDWHDYDMSNPPALIHTRAGARLMTFSPKNGYLYGVDAASNELRYRV
                     PVTRIENQDTEFSTRVHTHFCPGAGGGGEWNGAAYDPRTNLTFTAETDWCGSATRATD
                     AELKSVGVGSAWMGAGSHETFGRTDPISQWGGWVYATDADSGEWKWRARSNYPTLSGV
                     TPTAGGLVFLGDHGGNLYALNSSNGQPLWSLKVRGPIAGGIISYSAGGSQRIAVAYGM
                     TSALWVTVPSSAKIMILGLP"
     gene            6000..6086
                     /locus_tag="VNZ06_08855"
     tRNA            6000..6086
                     /locus_tag="VNZ06_08855"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6034..6036,aa:Leu,seq:taa)
     gene            6268..7380
                     /locus_tag="VNZ06_08860"
     CDS             6268..7380
                     /locus_tag="VNZ06_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWW20900.1"
                     /translation="MVARKSKLKPIPNADGSVSYVAQVRVKGFRPVAKSFCSADYEGA
                     AGAAKKAAEADAIQWREETIAELRKQSQQGAARSDISKLTIAGLNKEYLADPVTAAKA
                     YFGELKRNLSWWTSHYGTVRVLEFGVVHGRGARDKLMAGRGPATVDRYLSAQRSAWNF
                     GRKSGLVPKDRVWPPGLMLSEPDARVRYLDDEELPALLTAAEQAGPVLHAAILASIAC
                     GMRKGELLRLTWADIDFERSSVRILITKNGRARSVYLPTAAAAALKKLKASTVVSTTH
                     VFLNGEGKSLNWNELDKRWRPVRTAAKLENFRWHDLRHCCASFLAQSGASLLEIGSVL
                     GHRSAGVTLKYSHLIAGQAVTGHAKLNERLGGKTAS"
     gene            7377..7946
                     /locus_tag="VNZ06_08865"
     CDS             7377..7946
                     /locus_tag="VNZ06_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20901.1"
                     /translation="MTKPARRRRQQHLRLPHVVDSTRLVEPWLRVVDDAFGTVELRGS
                     LESYLKLSQVERLKPGESPPLAVMTIEDHRLKFPPIEYERYQSVKLQTFVDSLLRRVL
                     DGEDPRTVFALDKTKRGLKGNTARQIAATAEVARLVAEGFDRVEMTALVAEFFGTTPR
                     TVERWCAEWDDRKVNIAVYVRDLKTAGLL"
     gene            8045..8311
                     /locus_tag="VNZ06_08870"
     CDS             8045..8311
                     /locus_tag="VNZ06_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20902.1"
                     /translation="MARIPVPSVPHSWKLDDWPREIYPGAASKGRYVVRAHRTELIAR
                     GALVRIGRDLVVMGGPYASWMAQQGERVKEYQIAPNVRRAKAAA"
     gene            8382..8648
                     /locus_tag="VNZ06_08875"
     CDS             8382..8648
                     /locus_tag="VNZ06_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20903.1"
                     /translation="MSPTPAVYPPACIAQPDVDDASIALASAMDCWRDDAAAIEVALN
                     ALADLRVLLAYLPLSAAAAAIADDRIGEVELALRRFESPPGPIR"
     gene            8645..>9099
                     /locus_tag="VNZ06_08880"
     CDS             8645..>9099
                     /locus_tag="VNZ06_08880"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HWW20904.1"
                     /translation="MSAPELRPYQLGGLASIDAVRERGTNSLVAAAPTGSGKTIIAAE
                     YIRRQNDQGRRVLMLAPRRELVRQTVEKLDRFGVVPGVLLAGDRRQNLFAMTQVASID
                     TLRSRVRRLVFPDPHTIIVDEAHCYITRARTALIARWPNAFVLGLTATP"
BASE COUNT         1723 a         2731 c         2980 g         1665 t
ORIGIN      
        1 ccaggcagcc ggccatctcc gcggcctgct gcgctttggt cctgtcatcg aagtcgatcg
       61 cggagaatgg cagcagcaac gggcgcaggg gcgtgagcgt ggctcccggc agactgggat
      121 tgagcagcgc cagctgtccg cttgggtgct gcagtcgggc gccgatctgc cgcgtattgc
      181 gatcgaacag ctcgcggtag gcgcgatagc tgagctgctt ttcctcgcgc agttcgaaca
      241 gcgccagcgc gacggtggcg agcgcgagca acaggaaggc gctgcgaaag aacacccgca
      301 gccgcagcgg tacctgattg ggccaccaac cgctcacgca tcgacccagc ggaaaccgcg
      361 catcggcacg ttctcgatca ggtcgaacct cgggtccagt tcgcgaaacg cctcgcggat
      421 cgtgctgatg tgcttgcgca cgttgtcgcg gttgcggccg ctcttgacga cctggaacag
      481 ctcatcgtac gagacgatgc ggccgcgctt ctgatagaga gcatccagga tgcgctgcga
      541 ggtcagcggc agattgatgc gctggccacg ccacagcggc gaattctgtc gcagcggatc
      601 cagcgacagc tgatgcgtgg cgttggagac ggaggcaatc agcggcaggg atggatcctg
      661 catcgagcgc agcatgtcga ggaatgtctc gacgaattcg ttctcgtcaa agtcgctctt
      721 ctgcagatag tcccaggcat ccagcgcctt catgatgctg cgatagattc ccgctggaag
      781 cccggaaacc accagcaccg ggatcttgcg gcgcttgttg atcgcattga tgaccgccac
      841 gccggcgtga cgttcgcgtc cgagttcgat gtcgagcacg accaggtcat agggttcgcg
      901 atcgacggcg gaggtggcct catcgcgcgt gtaccactga tcgacccgga ttccggaacg
      961 agcgctttct atccagcctt tgatctggtt gctggtcggt acgtcatctt cgatgaccgc
     1021 cacccttacc atcgcgcctg tctgcctggt ttggttgtgc cgccacgata gcaatgggca
     1081 gctgaaccgt agctgactgg ccgtgaacga tccttccggc cgcggaacga agaggccccg
     1141 cgacggcatt gagcgctcca ccggcgcctt ccgatactgg cggcgacatc acaacaggag
     1201 tgctctcatg acggaccggt tcactgccat cctcgagttg ctgcgcgcga tgggtgcgag
     1261 catttctgca gccatggcca cggtcggggc cgggctcggg cggctgcgcg tgcagctgtt
     1321 ggtgctggca gcgctggtac tgatcgcttg gatgatcgtc aggcatccac cttttgcgtc
     1381 cgtgcagcgc ggagagatcg cggttcgcac caatctgctg accggacagg tctcgcagtt
     1441 tcgggaaggc agcctgtggg tgattcccgg attgcagcag gtgcgctatt tcccaacgcg
     1501 cgaccagatc tatcgccccg cacagagcgc caaggccaac ggtgaggcac cgttccagtc
     1561 ggtggaggga ttgtcgctcg gcgtggattt gacggtgcgc tacgcgatcg atccggcgcg
     1621 catggccagc atcgcgaaga cgctgccgga tgagctggat gatgtcattg agccggcggt
     1681 caagggggtg gtctacccgc tgtttgccgg ctacaccgtg aaggacatct tcaccaccag
     1741 gcgcgccgag atacaaaagg cgatcgagga gcagcttcga ccgaagctgg ctgccgacgg
     1801 ctttctgctg cgcagcgtgc agatgggaga ggtcgatcta ccacgcgact accggctcgg
     1861 catggagaag ctgctggccg aagagctcga gagcgagaag atgcgctaca cgctggagct
     1921 caaggacaag gaagtcaaag cgcgcgcact ggagggcgaa gccgacaagg ttcgccggca
     1981 gaaggccgcg gaggcggctg gcgaagagca ggtcatcgcc gccaaggcac aggaggaggc
     2041 catgaagcac gtcctgccct tcaagcagaa gcagatcgag cagcgccgtc ttgaggcgga
     2101 ggccgaaaag gtggcccgca tccgctcggc ggaaggtacc gcggatgcac ggcgcatcga
     2161 ggcctccggt gaggccgatt cgcgtcagaa gctcgcggac gctgaagtct accggctcga
     2221 aaagatcaac cgggccacca gtgagcagat gcagcgcgaa ggtgcgctga tcagtcagaa
     2281 cccgctgctg atccagaaaa cgatggcgga caaattgtcc gacaaggtgc aggtgatcat
     2341 tgctgcgcct ccggcagagg gcggtttcat cggttccaac ctattaggtg ccaaatgagt
     2401 gcgttactag cttcggcgat gcttgccatc gtcacccaga accaggttgc cctgcgcgcc
     2461 gcgccgcgtg acagcgctcc acaacaggcg atgctctggc agggagaggc gcttgatgtg
     2521 cgcgacgcgc gcggcgacta cctgctggta tacgacctga agctcgagcg cgaaggctac
     2581 atacgatcca atcaggtgcg gctgacgacc ctgaggcccg aagaggcccc cgagctgctg
     2641 gcggtggtgc gattcctgcg cgacaccccc ggctcggagg ctttggggat cagttatgcc
     2701 gcggcgtatc tgaaagcgat tcccgcttca gcaatgaccg ccgagccgct ggatgcgctg
     2761 ggcaatatgg cacaacgtct ggcgcagcgc gcctccagcg tgcagtcaaa gagcgcccag
     2821 gccacgatct cggcgcacct ggaagtggtc tcgcagatgg gtgtcacgat gacagggttc
     2881 gagcgtgatg gcagcgtgca gatctgctac gacggcgagg tgttcaggaa ggtgctggcg
     2941 atgtcgtcag ccgatccggc agaacttgcg cgtgcggcgc tcggactcac gcgccacgag
     3001 tgcgtggatc ccaacatggg accgatcgag cgttaccagt tcgatcagtg gcgagcagat
     3061 gtgctcgatc gcgtgccgac cgatgaactc attctggggc tgaagagccg ggtgctgatg
     3121 cggcgagcgg gtgtgctggc ttcgttggcc ttctggcaag cgcgccgcgg cctgaatcca
     3181 cagctcgccg ccgaacgctc gatcgaggat ctggccggtg tcgacaaatc ggtgctggac
     3241 gatgaaggct tgcgagacta cgccgaggcc gcggtgcgcg tcggcgcttc acggctggcg
     3301 gcactgaaga tggcgaatcg gggcggcaag ctggtggtgc atacctcggc cggcgcgccg
     3361 ggggagacct gcgtatcgct cttctacatg caaaagcagg atggcgaacc gctgatccgt
     3421 cgctgtacct atgggactgt gtggagcagc tcgatgagca gcaacgtgga aggcacggcg
     3481 ttgacgctgg cggtacagcc gctggcgacc tggcgcgagc tgtgggtgtt tcgcaaaacc
     3541 gcggacggct ggacggccaa tgtgctaccg ccggatctca acgggccggg gctcggctac
     3601 atcgaatcag ccggctggga cccgggcacg aagcgattgc tgctgtgtcg cgaagccctg
     3661 gtgagcgatc atctgctgcg acggttcgag ctggtgcggc tcgacacgct ggcgacggtg
     3721 catcaggcct cgaccccgga atatctcacc aactttgcgc gctggcagga cccggtctgg
     3781 cgcagtaaca ccgtggcgct gcgctgaagc gtcagggatg actgcgccac ccttggcgcc
     3841 aggtgacgaa cacggccgtc cagacgcaga tggtgcacct cgtccaggct ggcgaagtcg
     3901 atcggtgcca cctcgatgcc cgcgccgagc cagcgcccgg cgcgcatcct cggtggcggg
     3961 gtcttcgccg atgatatcaa tctagggcag ccccagaatc atgatcttcg cgctgctggg
     4021 gacggtcacc cataaagccg aggtcatccc gtaggcgacc gcaatgcgct gcgagccgcc
     4081 ggcggagtaa ctgatgatcc cgccagcgat cgggccgcgc actttgaggc tccacagcgg
     4141 ctgaccgttg ctgctgttca gcgcatacag attcccgccg tgatcgccca ggaataccag
     4201 tcccccggcg gtgggtgtca caccgctgag cgttggatag ttggagcgcg cgcgccattt
     4261 ccattcgccg ctgtcagcgt cggtcgcata cacccagccg ccccactgag aaatcggatc
     4321 ggtccgaccg aacgtctcgt gtgaacccgc ccccatccag gcgctaccca cgccgaccga
     4381 tttgagttcg gcgtcagtgg ctcgcgtcgc gctgccgcac cagtcggtct ccgcggtgaa
     4441 ggtgaggttg gtgcgcggat cgtaggcggc gccgttccat tcaccgccac ctccggctcc
     4501 cggacaaaaa tgagtgtgca cgcgggtgga aaactcagtg tcctgatttt cgatacgggt
     4561 gaccggcacg cgatagcgca gctcgttgct ggccgcatcg acgccgtaga gatagccgtt
     4621 cttgggcgaa aaagtcatca gtcgtgcgcc ggcgcgcgtg tggatgagcg cgggcggatt
     4681 ggacatgtcg tagtcatgcc agtcgcgcgg ctccatctga aagtgattga cgtagtcccc
     4741 ggtctttgca tccagtatca gcaccgagtc cacgtacagg tcactgcctg cgcgagcagc
     4801 gtcgacaaag tccggactcg gattgccgac cgggatgtac agccagccgg tggccggatc
     4861 cagggtatac gaggtccaag ttcccccgcc actgatcggc aggcccggcg tatcgcgatt
     4921 gtcccagctc tggagcgcgc cgtcgggaat cctgccggca ggcccgaaac tcggatcgcc
     4981 cgcctgtttc ggcaccagat aggtctccca gacaatgcgg ccgctgtcgg cggcgattcc
     5041 atacatgcgt cccttgatgc ctttggagtc cccgccgcag atgcccgcga acaccatgcc
     5101 gtcccaggca atcggcgctg aacaaatgtt ggtcccgagc ttgggatcgg ccaccgaggc
     5161 ttgccacagt cggtggccag tgtggctgtc gtaggccagt acgcgtccgt cgagcgttcc
     5221 ccggaaaaca cgtccttgta gataggccgc tccgcgagag gccagtaccg gcgagcgcgg
     5281 ggtgtagttc tcgtgcaccc gccagttctg cctgcaactg gacggatcga tcgagaaggt
     5341 atcgaaagcg ccggtggcga tcagtgcgcc gtccaccatg atcggtccgc tctcaaagcc
     5401 ctcgtggatc cccgagtcat agacgcacag cacgcgcagc gatttgatgt tctggcggtt
     5461 gatctgatca agcggggaga agcgctgcga ggtcagcgtc cggttatagc tcggccattc
     5521 gttgggatcc ggggtgtcag tgggcgcggc gccgagctcc ggcgccgggc ttccgggtcg
     5581 cacttcgacg ctcagtgtgc cgggcgggtt gctcagggtg cgaacggtat tcgtgaccgc
     5641 tcccagcagt gtgagcggcg tcggcatgat caagaggcca atgccggcag tcacgccgac
     5701 cagcagcaag gccgcgagca gaatccgacc cagagttttc atatcgcctg ttcttcagcg
     5761 aactcgttga atgcctccat gatcgcatgc acgtcggcca ggggatacgc agccgcgcgg
     5821 cgatcgagct ggcgcgaacg ccgttgagag agtgtcggat gacttcgcgc cgctcggcat
     5881 acccccttca agcccgacac acggaactga tgcataattg gcacacaggg tgagacgacc
     5941 ccgtgaaggt tgaccgaaca tcaggttcta gcagctgccc tgaaggcggg cgtcgaagcg
     6001 cgggcgtggc gaaatcggta gacgcagcag acttaaaatc tgccggcttt acggccatgt
     6061 gggttcgagt cccgccgtcc gcaccaatga cttagccgcg tttctgcaca ctgtgcacta
     6121 actcgatttg caccacgatt tgcactaaag attttctcgg cgtaccgtct gcgattgcgc
     6181 gtgctgggaa tgcagtaacg cgcgacaccg gagaggtgtt ggaagcacct ccccggcgtc
     6241 tggccacaac gcaacagagg tgcagtcatg gttgccagaa agtctaagct caaaccgatt
     6301 ccaaacgctg acggctctgt cagttacgtc gctcaagtgc gcgtcaaagg attccggccg
     6361 gtcgcaaagt cgttttgctc ggcggactac gagggcgctg cgggggcggc caagaaggct
     6421 gcagaagcgg atgcgatcca atggcgtgag gagacgatcg ccgaactcag gaagcaaagt
     6481 cagcagggcg cggcacgttc ggacatcagc aagctgacga tcgccggtct caacaaggag
     6541 tatctggccg acccagtgac agcggcgaag gcgtacttcg gggaactcaa gcggaacctc
     6601 tcctggtgga cgtcacacta tgggaccgtt cgcgtgctgg agttcggtgt cgttcacggg
     6661 cgaggagcgc gcgacaagct gatggctggc cggggcccgg ccacggtcga taggtatctt
     6721 tcggcgcaga gaagcgcgtg gaattttgga cgcaaatccg gattggttcc gaaggatcgc
     6781 gtgtggcccc ctggtctcat gttgagcgaa ccagatgcac gcgtacgcta cctggatgac
     6841 gaagagctgc cggcgctgct cacggctgcc gagcaggcgg ggccggtgct tcatgccgcg
     6901 atcctggcgt cgatcgcctg cggtatgcga aagggtgagc tgctgcgatt gacctgggcg
     6961 gatattgatt ttgagcgatc gtccgttcga atcctgatta ccaagaatgg ccgtgcacgc
     7021 agcgtctacc ttccaacggc agccgctgca gcactcaaga agctgaaagc ctcaactgtc
     7081 gtcagcacca cgcacgtgtt tctcaacggt gagggcaagt cgcttaactg gaacgaactc
     7141 gacaagcgct ggcggccagt gcgtacggcc gcgaagttgg aaaattttcg ttggcacgat
     7201 ctgcggcatt gctgcgcttc atttctggcg caatcgggcg cttcattgct tgagattggc
     7261 agcgtgctcg ggcaccgcag tgccggcgtg acgttgaagt acagtcattt gattgcaggc
     7321 caagccgtca ctggtcacgc gaagcttaat gagagattag gcggcaagac tgcatcgtga
     7381 ccaagcctgc caggaggagg cgtcagcaac acctgcggtt gccgcatgtg gtggattcca
     7441 ctcggctcgt ggaaccgtgg ttgcgggtgg tggacgatgc gtttggcacc gtggagcttc
     7501 gaggcagcct ggaatcctat ctgaagctat cgcaggtgga gagactcaag ccgggtgaat
     7561 caccacccct tgccgtcatg actattgagg accaccgctt gaagtttcca cccattgagt
     7621 atgagcggta ccaatccgtc aaactgcaga ccttcgttga tagcctcctg cgtcgagtgc
     7681 tcgacggcga agatccgcgc acggtctttg ccttggacaa gacaaaaagg ggtctgaagg
     7741 ggaacactgc gcgccagatc gcagcgacgg ccgaggttgc gcgtctggtc gccgaaggtt
     7801 tcgatcgcgt ggaaatgacc gcattggtgg cggaattctt cggaactact ccgcgaactg
     7861 tcgagcggtg gtgcgctgag tgggatgacc gcaaagtcaa cattgctgta tacgttcgcg
     7921 acctcaagac cgcggggctt ctctagcgcc gcgactctcc gacacaaatc gcgcgggtat
     7981 tgtgtcgtgc acatttccgt gcgtgctcat tagataggaa cactcaatct cttggagtgt
     8041 tcccgtggca agaatccccg ttccttctgt tccacattcg tggaagctcg atgactggcc
     8101 gcgcgaaatt tatccaggtg ccgccagcaa gggccgatac gtagtccgcg cacaccgcac
     8161 tgagctgatt gcccgcggtg ccctggttcg catcggtcgg gacttggtcg tcatgggtgg
     8221 cccctatgcg tcatggatgg cgcaacaggg cgagcgcgtg aaggagtatc agatcgctcc
     8281 caacgtgcgc cgcgccaaag cggcggcctg attcgggaga gtcggacgcg actgaccgcc
     8341 agttttcgag gcggacggcc agacgctgac gactccagac agtgagcccg acacccgcag
     8401 tgtatccgcc ggcatgcata gcgcaaccgg acgttgacga cgcaagcatc gcactggcga
     8461 gcgccatgga ctgctggcgc gacgacgctg ccgccatcga agtggcgttg aatgcgcttg
     8521 ccgacttgcg cgtactcctc gcttatctgc cgctttcggc cgccgccgcc gcgattgccg
     8581 atgaccgcat cggtgaggtc gagctggcac tgcgtcggtt cgagtcacca ccggggccga
     8641 tccgatgagc gccccggagc tgcggcccta tcagctcggc ggattggcat cgatagatgc
     8701 tgtgcgcgaa cgggggacca attccctggt ggctgccgcg ccgaccggca gcggcaagac
     8761 catcatcgcg gccgaataca tcagacgtca gaacgatcag ggccgccgtg tcctgatgct
     8821 cgcaccgcgg cgcgagctgg tgcgccagac cgtggaaaag ctcgatcgct tcggcgtggt
     8881 gccaggagtc ctgctggccg gcgaccgtcg tcagaacctg ttcgcgatga cacaggtcgc
     8941 cagcatcgac accctgcgat cacgtgtaag gcgtctagtg tttcccgatc cgcacaccat
     9001 catcgttgac gaagcgcatt gctacatcac gcgcgcacgc accgcgctga tagcacgatg
     9061 gccgaacgca ttcgttctgg gactgactgc cacgcccga
//
LOCUS       DATMRM010000300         8492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1420867, whole genome shotgun sequence.
ACCESSION   DATMRM010000300 DATMRM010000000
VERSION     DATMRM010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8492
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420867"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /gene="paaA"
                     /locus_tag="VNZ06_08885"
     CDS             <1..756
                     /gene="paaA"
                     /locus_tag="VNZ06_08885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HWW20905.1"
                     /translation="RKTILLAKVQDEAGHGLYLYCAAETLGQSRDQMLAALHSGKAKY
                     SSIFNYPTLSWADMGTIGWLVDGAAIMNQVPICRCSYGPYARAMIRICREESFHQRQG
                     YEGLLVMMQQGTKAQRAMVQNAVDRWWWPSVMMFGPPDAQSVHSAQSMRWGIKRVSND
                     ELRQKFVDASVEQARVLGVTLPDPAIQWNEARGHYDFGTIDWNEFWRVIAGHGPCNRE
                     RLRARVKAWDDGAWVREAALAYARKHAARSEMA"
     gene            753..1052
                     /gene="paaB"
                     /locus_tag="VNZ06_08890"
     CDS             753..1052
                     /gene="paaB"
                     /locus_tag="VNZ06_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HWW20906.1"
                     /translation="MSDPSQANEWPLWEVFVRSKSGLDHKHCGSLHAADAKMAIQAAR
                     DVYTRRQEGTSVWVVRSDQIIASDPGDKSMYFDPMVDKVYRHPTFYQLPQSVDQM"
     gene            1052..1858
                     /gene="paaC"
                     /locus_tag="VNZ06_08895"
     CDS             1052..1858
                     /gene="paaC"
                     /locus_tag="VNZ06_08895"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HWW20907.1"
                     /translation="MNERPSSIEPGRSPAVQYVLRLGDACLILAQRLGEWCGHAPVLE
                     EDIALTNTALDLIGQARALLTHAGQLSAAERGGAALDEDQLAFLREERDYLNPTLVEL
                     PRGDFALTTLRNAMVATWLQLLWQRLGASSDAELAGIAAKAVKEARYHQRHAADWVVR
                     LGDGTEESKRRLADALQELWPYSAELFETDAVDEYAAATGLGPRWSDLRQEWLSQMSA
                     LLTEAGLAIPADVPFRSTGRTGRTGQHSEHLGYLLTDLQYLQRAYPGGVW"
     gene            1855..2388
                     /gene="paaD"
                     /locus_tag="VNZ06_08900"
     CDS             1855..2388
                     /gene="paaD"
                     /locus_tag="VNZ06_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HWW20908.1"
                     /translation="MSAIGITHSSRVERAWRVLQQVLDPEVPALSVCELGIVREVLDH
                     GQSLEIVVTPTYSGCPATEVIEQRILEAIRAAGLGPARLRLQRAPAWSSDWISAEGRR
                     KLHDYGIAPPGPAATQAEAVVSVGSIRRRGAAPHVACPRCASTQTTLVSGFGSTACKA
                     LFRCLACLEPFEYFKPI"
     gene            2393..3478
                     /gene="paaE"
                     /locus_tag="VNZ06_08905"
     CDS             2393..3478
                     /gene="paaE"
                     /locus_tag="VNZ06_08905"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: protein motif:HMM:TIGR02160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HWW20909.1"
                     /translation="MSVLFHPLRVRSVEADTPEAVVVCFEVPQELRATFGFIQGQYLT
                     LRKRIDGQDLRRSYSICAGLDEGELRVGVRRVQGGTFSNWINTRLKAGDTLQVMAPQG
                     RFFVPLDPATHRHYVGIAGGSGITPILSIMKTVLAREPHSEFTLIYGNRALRSTMFKE
                     EVDDLKNRYLSRLAVHHVFSDEHADSPLNGGILNREKITEFLQQVIAPSVIDHAFICG
                     PFQMNDEAQAALLAVGVAEDRIHIERFGLAPQTDSAVGAVVHQPSPDDAERSRVTIIR
                     DGIRREFEFLQGQPSILDSASAAGIEVPFSCTSGVCGTCRARLMEGQVRMERCYALDK
                     AQVAEGFVLTCQSHPLTERVVLSFDER"
     gene            3491..4114
                     /locus_tag="VNZ06_08910"
     CDS             3491..4114
                     /locus_tag="VNZ06_08910"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWW20910.1"
                     /translation="MARTRDAGYEDQRRMILAHAAHLFARQGYAATSMNEVADACGLA
                     KASLYHYYRDKYSLLNSIADTHVSRLRAVVAEVGESGLAPRQELRELIRRFLAEYSDA
                     KDAHRVLTEDVRFLAAADRARVLGKEREVVAGFANAIARVRPQLRRAALVKPLTMLLF
                     GMINWMFTWMNAEGPLDYDAMAPVVADLFLGGVGAVKLTRDVATSIG"
     gene            4119..6164
                     /gene="paaZ"
                     /locus_tag="VNZ06_08915"
     CDS             4119..6164
                     /gene="paaZ"
                     /locus_tag="VNZ06_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HWW20911.1"
                     /translation="MTNTLQSYIGGRWFGREGNETLRSAVNGRPVSMTHRERIDFAEV
                     VHHARSVGLPGLLALDFQQRAARLKALAKFLGDHKEELYGLSTQTGATRADSAIDLEG
                     GIGTLHAYASVGSNELPSGNLLHEGPAMMLGKKGGFAGTHILVPRRGVAVHINAFNFP
                     VWGLLEKFAPSFLAAMPCIVKPATSTSYLTERLVRLMQNSAVLPEGSLQLVIGPIGDL
                     FERLEGCDVVSFTGSADTAARLRVHPNLIRHSIPFNAEADSLNCAILAPDVTPDDQEF
                     ELYLNEIVREMTAKAGQKCTAIRRALVPRAQLEAVALRLKQRLAAIVIGDPASEDVRM
                     GALASVAQQRSVAQQVEALLKESELIFGSRDGFDPQGEGVREGAFFAPTLLLCRDPIR
                     ASGVHEVEAFGPVTSLLPYEGIDEALALAVRARGSLVGTLVTHDAATAARAIPIFAAW
                     HGRLLILDREAGAESTGHGSPLPSLKHGGPGRAGGGEELGGLRAVTHYLQRCAVQGSP
                     SMLTAVTGEYVRGGAVKESEVHPFRRHFEELQIGESLLTHRRTVGESDISAFGGISGD
                     YFYMHFDEIAARDSAFGQRIAHGYFVLSAAAGLFVSPAPGPVLANYGLDTLRFVKPVG
                     IGDTIRARLTCKRKIDRQRKDAKGIGQGVVAWDVQVTNQNDELVASYDILTLVAKRG"
     gene            complement(6389..7732)
                     /locus_tag="VNZ06_08920"
     CDS             complement(6389..7732)
                     /locus_tag="VNZ06_08920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW20912.1"
                     /translation="MSKSARAWIALFILFAINLLNFFDRQLLGSLGEPIRKEFHLSDT
                     GLGTLGTVFTIMYAIVGLPVGRLSDKWSRTRLIALGTGVWSLLTAASGFVQNFTQMFV
                     CRLGVGVGEAVCAPAGQSLIGDLFPPVQRARAMGIFMMGLPLGLFLAYMSVAFIAPHY
                     GWRMVFKFAIIPGLLLALAALFLHEPIRGALDPARKAASATVSAPSMSPWKILSLPTM
                     WWIIASGILHNFNMYAIGGFSAPFLQRYHALSLFNANMTASVSSGLVGAVGLLVGGYL
                     GDKMSAKRRNGRLLLAACAMTIAAPCIFFAVDQPKGSVVAFVILMSLGSATMYVYYAT
                     VYAAIQDVIEPQLRGIAVAMYFCAMYLLGASLGPAGLGMLSDHFAHEAMRQAGATSMA
                     EAFRATGLHNAMYVIPIFALLAAGVLFAASRTVERDIRKQEAGHAALDAAAVPLT"
     gene            7947..8372
                     /locus_tag="VNZ06_08925"
     CDS             7947..8372
                     /locus_tag="VNZ06_08925"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWW20913.1"
                     /translation="MSNSDFPRVPHPFINDLGVEFLDSGAGRSRVALQLTQRHLNSWS
                     VAHGGVIMTLLDVAMAIAGRSLDTHAGGGVTVEMKVSFLQPAIESSRLIATGHAFHQS
                     KTMAFCEGEIHDAQGRLIAKSLGTFKYIRKRPTDQPADG"
BASE COUNT         1509 a         2592 c         2888 g         1503 t
ORIGIN      
        1 cgcaagacca tcctgctggc caaagtgcag gatgaggccg gtcacggcct gtacctgtac
       61 tgcgcggccg agacgctcgg gcagagccgc gatcagatgc tcgccgcgct gcattccggc
      121 aaagccaagt acagctcgat cttcaactac ccgacgctga gctgggccga catgggcacg
      181 atcggctggc tggtcgacgg cgcggcgatc atgaaccagg tgccgatctg ccgctgctcg
      241 tacgggccgt acgcccgcgc gatgatccgc atctgccggg aagagagttt tcatcagcgc
      301 cagggatacg aggggctgct ggtcatgatg cagcagggga ccaaggccca gcgcgcgatg
      361 gtgcagaacg cggtcgatcg ctggtggtgg ccgagcgtga tgatgttcgg tccgccggat
      421 gcgcagagtg tgcacagcgc gcaatccatg cgctggggca tcaagcgcgt ctcgaacgat
      481 gagctgcgac agaagttcgt ggacgccagc gtcgagcagg cgcgggtgct cggtgtcacg
      541 ctgcccgatc cggcgatcca gtggaatgaa gcacgggggc actacgattt cggcacgatc
      601 gactggaacg aattctggcg ggtgatcgcc ggccatggtc cgtgcaatcg cgagcggctg
      661 cgcgcgcgcg tcaaagcctg ggacgacggt gcctgggtgc gcgaggcggc gctcgcgtat
      721 gcgcgcaagc acgctgccag gagtgagatg gcatgagtga tccaagtcag gcgaacgagt
      781 ggccgctgtg ggaagtgttt gtgcgcagca aatcgggact cgaccacaag cactgcggca
      841 gtctgcatgc cgcggatgcg aagatggcga ttcaggcggc acgcgatgtg tacacgcgcc
      901 gtcaggaggg caccagcgtg tgggtggtgc gctcggatca gatcatcgcc agtgatccgg
      961 gtgataagtc gatgtacttc gacccgatgg tagacaaggt ctatcgtcac ccgaccttct
     1021 accagttgcc gcaatccgtg gaccagatgt gatgaacgag cggccgtcat cgattgaacc
     1081 cgggcgctcg ccagccgtgc agtacgtgct gcggctggga gatgcctgtc tgatcctggc
     1141 gcagcgctta ggggagtggt gcggccacgc accggtcctg gaagaggaca tcgcgctcac
     1201 caacaccgca ctcgacctaa tcggtcaggc gcgcgccttg ctgacgcacg ccgggcaact
     1261 cagtgccgct gaacgcggtg gcgctgcgct cgatgaggat cagctcgcgt ttctgcgcga
     1321 agagcgcgac tacctcaatc ccacgctggt ggagttgccg cgtggggact tcgccttgac
     1381 cacgctgcgc aatgcgatgg ttgcgacgtg gctgcagctg ctgtggcagc gcttgggcgc
     1441 atcgagcgac gccgagctgg cgggcatcgc ggcgaaagct gtcaaggagg cgcgctatca
     1501 ccagcgccac gccgcggact gggtggtgcg gctgggagat ggcaccgagg aatcgaagcg
     1561 tcgcctggcc gacgcgctgc aggagctgtg gccttacagc gcggagctgt ttgaaaccga
     1621 tgccgtggac gagtatgcgg cggcaacggg cttaggtccg cgctggtcgg acctgcgtca
     1681 agagtggctc tcacagatga gtgcgctgct cacggaagcc ggacttgcga ttccggcgga
     1741 cgtaccgttt cgcagcaccg gtcgcaccgg tcgcaccggt caacacagcg agcatctggg
     1801 atacttgctc accgatctgc agtatctgca gcgcgcctat ccgggagggg tgtggtgagc
     1861 gcgatcggca tcacgcactc gtcgcgcgtc gagcgtgcct ggcgcgtgct gcagcaggtg
     1921 ctcgacccgg aggtaccggc gctgtcggtg tgcgagctcg gcatcgtgcg cgaggtgctc
     1981 gatcacgggc agtcactcga gattgtagtg acgccgacct attcgggctg cccggccacc
     2041 gaggtcatcg aacagcggat tctggaggcg atccgggccg caggcttggg tccggctcgc
     2101 ctaagactgc agcgcgcgcc cgcctggagc agcgactgga tcagtgccga gggccgccgc
     2161 aaattgcacg attacgggat cgccccgcca ggcccggccg cgacgcaagc cgaagcggtg
     2221 gtatccgtgg gcagcattcg ccgccgcggc gcagcgccgc acgttgcctg cccgcggtgc
     2281 gccagcaccc agaccacgct ggtgtcaggg tttggttcga ccgcgtgcaa ggctttgttt
     2341 cggtgtctgg cctgtctgga accgtttgaa tacttcaagc cgatctagcc tcatgagtgt
     2401 gctgtttcac ccgctgcgcg tgcgttcggt cgaagccgat acccccgagg ccgtggtggt
     2461 gtgcttcgag gtgccgcagg agctgcgcgc gaccttcggt ttcattcagg gccagtacct
     2521 gacgctgcgc aagcgcatcg atggacagga cctgcgtcgt tcgtattcga tctgcgccgg
     2581 gcttgacgaa ggtgagctgc gggttggggt gcgccgcgtg cagggcggca ccttctcgaa
     2641 ctggatcaac acccgcctga aggcgggcga tacccttcag gtaatggcgc cgcaggggcg
     2701 cttcttcgtg ccgctggatc cggcgacgca tcgccactac gtgggtatcg ccggtggcag
     2761 cggcatcacg ccgattctgt cgatcatgaa aacggtgctg gcgcgcgagc cgcacagtga
     2821 gttcacgctc atttacggca accgagcgct gcgctcgacg atgttcaagg aggaggtgga
     2881 tgatctgaag aatcgctacc tctcgcggct ggccgtgcac cacgtgttct ccgacgagca
     2941 cgcggactcg cccctgaatg gcggcattct gaaccgcgag aaaatcaccg agttcctgca
     3001 gcaggtgatc gccccgtccg ttatcgatca cgccttcatc tgcggtccgt ttcagatgaa
     3061 cgatgaagcg caggccgcat tgctcgcggt gggagtcgcg gaggatcgca tccacatcga
     3121 gcgcttcgga ctggcgcccc agaccgacag cgcagtcggt gcagtggtgc atcagccgag
     3181 ccccgatgat gcggagcgct cgcgcgtcac gatcattcgg gatggcatcc ggcgtgagtt
     3241 cgaatttctt caaggtcagc cgagcattct ggacagcgcc tcggccgccg gcatcgaggt
     3301 gccgttctcc tgtacctccg gagtctgtgg cacctgtcgc gccaggctca tggagggcca
     3361 ggtgcgcatg gagcgctgtt acgcgctcga caaggcccaa gtggcggaag gattcgtcct
     3421 cacctgccag tcgcatccgc tgaccgagcg cgtggtgctg tcattcgatg aacgctgaca
     3481 ggatgcattg atggcacgca cccgtgacgc cggctatgag gatcagaggc ggatgattct
     3541 ggcgcacgcg gcccatctgt tcgcgcgtca gggttatgcc gcgacctcga tgaacgaagt
     3601 ggccgatgcc tgcggactgg cgaaggccag cctctatcac tactaccgcg acaaatacag
     3661 tctgctcaac agcatcgccg atacccatgt gtcgcggctg cgcgcggtcg tggccgaagt
     3721 cggcgaatca gggctcgccc cccggcagga actgcgcgag ctgatccgcc gcttccttgc
     3781 cgaatactcg gatgcgaagg atgcgcaccg ggtgctcacc gaggacgtgc ggtttctggc
     3841 agctgccgac cgcgcgcggg tcctgggcaa ggagcgtgaa gtggtcgcag gctttgcgaa
     3901 cgcgatcgca cgcgtgcgcc cgcagctgcg gcgcgcagcg ctggtcaaac cgctgaccat
     3961 gctgctgttc ggcatgatca actggatgtt cacgtggatg aatgcggagg gtccgctcga
     4021 ctatgacgcg atggccccgg tggtcgcgga tctgttcctg ggcggtgtgg gcgcggtcaa
     4081 actcacgcgc gacgtcgcga cgtcgatagg ataggagcat gacgaatact ctgcagagct
     4141 acatcggcgg ccgctggttc ggtcgcgagg gcaacgagac cctgcgcagc gcggtgaacg
     4201 gccgcccggt ctcgatgacg catcgggaga ggatcgattt tgctgaagtg gtccaccacg
     4261 cgcgcagcgt gggcctgccg gggctgctgg cgctcgactt tcagcagcgc gcggcacgtc
     4321 tgaaggcgct ggcgaagttc ctcggcgatc acaaggaaga gctctatggc ctgtccaccc
     4381 agaccggtgc cacgcgcgcg gacagcgcca tcgatctgga gggcggtatc ggcacgctgc
     4441 acgcctatgc cagcgtcggc agcaatgagc tgccctcggg caatctgctg cacgagggcc
     4501 cggcgatgat gctcggcaag aagggcgggt tcgccggcac tcacattctg gtgccgcgcc
     4561 gtggcgtcgc ggttcacatc aatgcgttca atttcccggt gtggggactg ctggagaagt
     4621 ttgcaccgag ctttctggcg gcgatgccat gcatcgtgaa gccggcgacc tccaccagtt
     4681 atctcaccga gcgactggtg cggctgatgc agaacagcgc tgtgctgccg gaaggcagcc
     4741 tgcagctggt gatcggtccg atcggcgatc tgttcgagcg gctggaaggc tgtgatgtgg
     4801 tgagctttac cggttcggcc gacacggcgg cgcggctgcg cgtgcacccg aacctgatcc
     4861 gccacagcat cccattcaat gccgaagccg actcgctcaa ctgcgcgatc ctggcgccgg
     4921 atgtgacgcc ggacgatcag gagttcgagc tgtacctgaa cgagatcgtg cgcgagatga
     4981 ccgcgaaggc cggacagaaa tgcaccgcga tccggcgcgc gctcgtgccg cgcgcacagc
     5041 tggaggcggt ggccttgcgc ctgaagcaac gcctcgccgc gatcgtcatc ggcgacccgg
     5101 cgagcgaaga cgtgcgcatg ggggcactgg cctcggtcgc gcagcagcgc tcggtggcac
     5161 aacaggtcga ggcgctgctg aaagaatcgg aactcatctt cggctcgcgc gacggtttcg
     5221 atccgcaggg cgagggggtg cgcgaggggg ccttctttgc gcccacgctg ctgctctgtc
     5281 gtgatccgat tcgcgcgagc ggcgtgcatg aggtggaagc cttcggtccg gtgacctcgc
     5341 ttctgcccta tgaaggcatc gatgaagcgc tcgcgctggc ggtgcgcgct cgcggcagtc
     5401 tggtcggcac cctggtcacc catgatgcgg ccaccgccgc gcgcgccata ccgatcttcg
     5461 ccgcctggca cggacgcttg ctgatactgg atcgcgaggc gggtgctgag tccacgggcc
     5521 atggctcacc gctcccgtcg ctcaagcacg gcggtccggg ccgtgccggt ggcggtgagg
     5581 agctcggggg actgcgcgcg gtgacacatt atctgcagcg ctgcgcggtg cagggatcac
     5641 cgagcatgct gacggccgtt accggcgaat acgtgcgtgg cggggcggtg aaggaaagcg
     5701 aggtgcatcc cttccggcgc cacttcgagg agctgcagat cggtgagtcg ctgctcacac
     5761 accggcgcac cgtgggcgaa tccgacatca gcgcgtttgg cggaatctcc ggggattatt
     5821 tttacatgca cttcgatgag atcgcggccc gcgacagcgc attcggccag cgcattgcgc
     5881 acggctattt cgtgctgtcg gcggcggctg gtctattcgt atcgcccgcg cccggtccgg
     5941 tgctggcgaa ctatggactc gacacgctgc gcttcgtgaa gccggtgggc atcggcgata
     6001 ccatccgcgc gcgcctgacc tgcaagcgca agatcgaccg ccagcgcaag gatgccaaag
     6061 gcatcgggca gggcgtggtc gcgtgggatg tgcaggtcac caatcagaat gatgagctgg
     6121 tggcctccta cgacatcctc acgctggtgg ccaagcgcgg ctaagccttg tccttcaggt
     6181 ccgcgcaacg gctcgcgggc tgttgaggac gctggcggcc atgattgctc cgtgctcggt
     6241 gaaggcgtaa ggcgcgaatc tggggtcacg gtacttttgc gtaccggtcg caaatttgcg
     6301 accggttcag cgctccgatc tgagctctag tgagccggaa catgaagtct tccgggaagc
     6361 gctcgcggtt gcgagacgcg attttcctct aggtcagcgg gaccgccgcg gcatccagcg
     6421 ccgcgtgacc ggcttcctgc ttgcgaatgt cacgctcgac cgtgcgcgag gctgcaaaca
     6481 gtaccccggc ggcaagcagc gcaaagatcg ggatgacgta catcgcgttg tgcaggccgg
     6541 tggccctgaa agcttcggcc atgctggtgg ctccggcctg ccgcatggcc tcgtgggcaa
     6601 agtgatcgct cagcatccct aggccggccg ggcccaagct cgcgcccagc aggtacatcg
     6661 cgcagaagta catcgcgacc gcaatgccgc gcagttgcgg ctcgatcacg tcctggatcg
     6721 cggcgtagac cgtcgcgtag tagacgtaca tggtcgcgct gcccaggctc atcaggatca
     6781 cgaacgcgac cacggatccc ttgggctgat cgactgcgaa gaagatacac ggcgccgcga
     6841 tggtcatcgc gcacgccgcc agcagcagtc ggccgttgcg gcgcttggcg ctcatcttgt
     6901 cgcccagata gccgccgacc agcaggccca ccgccccgac caggccggaa gagaccgatg
     6961 ccgtcatgtt ggcattgaac aggctcagcg cgtggtagcg ctgcaggaac ggcgcggaga
     7021 agccgccgat cgcgtacatg ttgaagttgt gcaggatgcc ggaagcaatg atccaccaca
     7081 tcgtcggcag gctcaggatc ttccagggcg acatgctcgg tgcggaaacc gtggcgctcg
     7141 cggccttgcg cgccggatcg agcgcgccgc ggatcggctc gtgcaggaac agtgcggcga
     7201 gtgccagcag cagtccgggg atgatcgcga acttgaatac catgcgccag ccgtaatgtg
     7261 gcgcgatgaa tgcaacgctc atgtaggcga ggaacaagcc cagcggcagt cccatcatga
     7321 agatgcccat ggcgcgtgcg cgctgcaccg gcgggaatag atcgccgatc aatgattgac
     7381 cggccggtgc gcagacggct tcaccgacgc cgacgcccag acggcagaca aacatctgcg
     7441 tgaagttctg cacgaagccg gacgctgcgg tgagcaggct ccaaacaccg gtgccgaggg
     7501 cgatcaggcg cgtgcgtgac catttgtcgc tgaggcgacc caccggcagg ccgacgatgg
     7561 catacatgat cgtgaacacg gttccaagcg tcccgagtcc ggtgtcgctg agatggaact
     7621 ctttgcggat cggctcgccg agtgagccga gcagctggcg atcgaagaag ttgagtaggt
     7681 tgatggcgaa taggatgaac agagcgatcc aggcgcgcgc ggacttgctc atggtagtgg
     7741 tcgatccccg aaagtgatga gcggccgcgc gtagctgctg taatattcta cgaaatcagc
     7801 ctctgagccc gataatctcc cggcgcgcgc aagcaagtca aggtgacgga ttttgttcaa
     7861 cgccgccgct agtagaggtt cagtctaccg ggcggctggt attgttacca actgtaatga
     7921 ccgcttgggt tagacttcgg cgcactgtga gcaattcaga cttcccgcgc gttccgcatc
     7981 cgtttatcaa cgatttaggg gtggagtttc tggactccgg cgctggccgc agccgggttg
     8041 ccctgcagct cacccagcgg catctgaata gctggagcgt ggcgcacggc ggcgtcatca
     8101 tgacgctgct cgacgtcgcg atggcgatcg cgggccgcag ccttgacacc catgccggtg
     8161 gcggtgtcac ggtggaaatg aaggtcagtt tcctgcaacc ggccatcgaa agttcacgtc
     8221 tgattgcgac cggccatgcg tttcatcagt caaagaccat ggcgttctgc gagggcgaga
     8281 tccacgatgc gcaagggcgc ctgatcgcca agtcgctcgg gacgttcaaa tacatccgca
     8341 agcgacccac ggatcaacct gccgacggct gagcattctt gctagcattc agtgaaacgc
     8401 cagccgattg gccgggcagc tgacagggga atccacatgc gtccgatcct gcaccactat
     8461 ccgacttcac ccttcgccga gaaggcccgc ac
//
LOCUS       DATMRM010000301         5491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2958782, whole genome shotgun sequence.
ACCESSION   DATMRM010000301 DATMRM010000000
VERSION     DATMRM010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2958782"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VNZ06_08930"
     CDS             complement(<1..863)
                     /locus_tag="VNZ06_08930"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HWW20914.1"
                     /translation="MIGSPIVGVIGVGVLLLLLASGVPIGVALGLVGFGGLSLLLGLE
                     PALIKSGVILFETLSRYELGVLPLFLLMAHLCFAANASRDFFDAAANLVGHRKGGLAL
                     AAVGACAGFGSISGSSLATAATVGLVALPEMRQRGYSSALATGAVAAGGTLGQMVPPS
                     GALIVFGIVAEQSIGRLFIAAIVPALTQLLLYCLVIVLLCLLRPTLAPATPRADWSQR
                     RKALFRVTDLGLLVLLVIGGIALGWFTPPEAASVGVLGALLLCARRRRLNGSSLQHAL
                     AETLGTSGMIYL"
     gene            complement(860..1312)
                     /locus_tag="VNZ06_08935"
     CDS             complement(860..1312)
                     /locus_tag="VNZ06_08935"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HWW20915.1"
                     /translation="MMASRGPLFWTGAAALLIVTGVDAAAVLGRHIGPPFPGSLELVQ
                     AAILLASTAGLVAATAVNRHASARLVLDRVPAVMRGWMLRLNALMSVLFFLLLAAGQL
                     WITSDLWHAHEDSDVLHIPFAPLRVLCVVGVLLAALIVSRRMIRPDQS"
     gene            complement(1309..2415)
                     /locus_tag="VNZ06_08940"
     CDS             complement(1309..2415)
                     /locus_tag="VNZ06_08940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HWW20916.1"
                     /translation="MDACDLLFTGDLVLDVPDADYWLSGIAAATRAAAVSIGHLEVPH
                     TRGPSVLHTDVPAPGADPRNLDALKAAGFSALTLAGNHIADCGAAGIADTIGRLEQLG
                     IRHCGAGANLTLARAPAHIQIAERTLALLSYNCVGPEEAWASEDRAGCAYVRINERAG
                     GRITPSAALTQPDARSLQAMQQDIRQARSDHDWVVVALHKGIVHTRARLAPYERQVAH
                     AAIDAGATLVVGHHAHILRGIEFYRERPIFHGLGNGCVVTRALGPDQSDPARAAWVQR
                     RRELFGFEPDPAYTLAPFHPEAIHALLARVRCSADGRVEAAFNPVFVEPPGRPVLAGN
                     DFRQSVMDYVSRITQEAGLPSLSMSVREDRIVVS"
     gene            complement(2546..3037)
                     /locus_tag="VNZ06_08945"
     CDS             complement(2546..3037)
                     /locus_tag="VNZ06_08945"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWW20917.1"
                     /translation="MPREQALKPSPRRRKQALAIAPVPINVGMLPNLLGYNVRRAQIA
                     LWRDLARSLGEVKVRPAEFSMMVLVDSNPGIAQIELANQLDVDKATIVGLVDQLQRQK
                     WAVRKQSTVDRRRQGIYLTALGQRELRRLEQTMIEHEARFTRLFSSEELTQLFAYLRR
                     IHP"
     gene            complement(3146..4519)
                     /locus_tag="VNZ06_08950"
     CDS             complement(3146..4519)
                     /locus_tag="VNZ06_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWW20918.1"
                     /translation="MKRVLTLATTLFCLMATASLASAQQIAGDWQGSLDTGAGKLRLV
                     LHITQGADGNLQSTLDSLDQGANAIPVASTTLKNSSLHLDVAAVRGSYDGTVSADGNS
                     ISGQWSQGLELPLTLTRVTDKSALVIKHPQVPVRPYPYHEEELTYENKAQNVTLAATL
                     TIPQGKGPFTAVLLISGSGPQDRDESLLGHKPFLILSDYLTRHGIAVLRADDRGVGKS
                     SGTFSTATTADFATDAEAGIAYLKTRVEVNPHKIGLIGHSEGGTIAPMIAARRKDVAF
                     IVMMAGTGVPGDQVIPAQVEAIALAGGKSAEEAAKAAAKERDILKLVETEKDEAVLEK
                     QLREKVAGEIPEAQIGMQLKAITSPWYRYFLTYDPASALRHVSCPVLAINGSLDRQVL
                     PDQNLPAIRQALQAAGNTHAQIDELPGLNHLFQTATTGSPSEYARIEETLSPLALEKM
                     ASWIAKR"
     gene            complement(4548..>5491)
                     /locus_tag="VNZ06_08955"
     CDS             complement(4548..>5491)
                     /locus_tag="VNZ06_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20919.1"
                     /translation="NVPLWWQLYRLRHALEVDCDARVLDAGHDLQAYGEALIAVGQQR
                     SAFIGAVAAMAESRTLLERRIEVMTARTHRRWTYGFALASILALSVAAAATQVTAPEA
                     PASQQQISVDATTLDRYVGNYQLGQRLLSVTRDGAQLSVQVSGQPKLPVYPETASKFF
                     WKIADIQMTFDSTGTGPSMSASIHQAGEDVVVLRVDEAAASRLAQQLEARSAAQQPQQ
                     GSEAALRKYIQGLESGTPDYDEMAPLIQSVTRRQLPLFQGQFARLGPIASVEFKGVSP
                     QGMDMYVVTHEGGQQSQWIISVNADGKIAEVGVLPRF"
BASE COUNT          981 a         1781 c         1780 g          949 t
ORIGIN      
        1 agatagatca tgccgctggt acccagcgtc tcggccagcg cgtgctgcag cgaactcccg
       61 ttcagccggc ggcggcgcgc gcacagcagc agcgcgccga ggactcccac ggaggccgct
      121 tccggcggag tgaaccatcc gagcgcgatt ccaccgatga ccagcagcac cagcaagccg
      181 agatcggtga ctcgaaacag cgccttgcgt cgctggctcc agtccgcgcg cggcgtggcc
      241 ggagcaagcg tcggtcgcag caggcacagc agcacgatga ccaggcagta gagcagcagt
      301 tgcgtcagcg ccggcacaat ggcggcgatg aacagccgac cgatcgactg ctcggcgacg
      361 attccgaaca cgatcagtgc cccggatggt ggcaccatct gtccgagtgt gcccccggcg
      421 gcgacagcac cggttgcgag cgcgctcgaa tagccccgct gtcgcatctc cggcaatgcc
      481 accagaccga cggtcgccgc ggtcgccaga ctcgagccac tgatggaacc gaaacccgcg
      541 cacgcaccga ccgctgccag cgcgagcccg cctttgcggt gtccgaccag attggcggcg
      601 gcatcgaaga aatcgcggct ggcattggcg gcgaagcaca gatgcgccat cagcagaaac
      661 aacggcagca cgcccagttc atagcgcgag agcgtctcga acagaatgac gccggatttg
      721 atgagcgccg gttccaaccc cagcagcaga ctcaggcccc cgaatcccac cagcccgagt
      781 gcgaccccga tcggcacacc gctcgccagc agcagcaaca gcacgcccac tccgatcaca
      841 ccgacaatgg gcgagccaat catgattggt cgggacggat catccggcgc gacacgatca
      901 gcgccgccag cagcaccccc acgacacaca gcactcgcag cggcgcgaag gggatatgca
      961 gcacgtcgct gtcctcgtgg gcgtgccaca gatccgatgt gatccagagc tggcctgccg
     1021 ccaacagcag aaagaacagc acgctcatca gtgcattcag ccggagcatc cagcctcgca
     1081 tgacggcggg cacgcgatcg agcacgaggc gcgcggaggc atgacggttg acggccgtgg
     1141 ccgcgaccag gccggcggtg gaggccagca ggatggccgc ctgcaccagt tccagcgagc
     1201 ccggaaacgg cggaccgatg tgccggccga gcaccgccgc ggcatcgacc ccggtcacga
     1261 tcagcagcgc ggccgcgcca gtccagaaca gcggaccacg cgaagccatc atgacacgac
     1321 gatcctatcc tcgcgcacgc tcatcgacag cgacggcagt ccggcctcct gcgtaatacg
     1381 gctgacatag tccatcacgc tctgacgaaa gtcatttccc gccagcaccg gccgcccggg
     1441 gggctcgacg aacacgggat tgaaggcggc ttcgacgcgt ccatcggcgc tgcatcgcac
     1501 gcgcgcgagc agcgcgtgaa tcgcctccgg gtgaaacggc gcgagcgtgt aggcgggatc
     1561 gggctcgaac ccgaacaact cgcggcgtcg ttgaacccag gccgcgcgcg cgggatccga
     1621 ctggtcgggt ccgagcgccc gggtgacgac gcagccgttg cccagcccgt ggaatatcgg
     1681 gcgctcgcga tagaattcga taccgcgcag gatgtgtgcg tggtgaccta ccaccagcgt
     1741 cgcgccggca tcgatcgcgg cgtgagcgac ctgtcgttcg tacggagcca gcctggcacg
     1801 ggtatgcacg atgcccttgt gcagggcgac gaccacccag tcatgatccg acctcgcctg
     1861 ccggatatcc tgctgcatgg cctgaagcga gcgtgcatcc ggctgcgtca gcgcggcgga
     1921 cggggtgatg cgcccgccag cgcgctcatt gatgcgcaca taggcgcagc ccgcgcgatc
     1981 ctcgctcgcc caggcctcct ccggtcccac acagttatag ctgagcagtg cgagcgtgcg
     2041 ctcagcgatc tgaatgtgcg cgggcgcgcg cgccagggtc aggttggctc cggccccgca
     2101 gtgccggatg cccagctgct ccagccggcc gatggtatcg gcgatacctg ctgcgccgca
     2161 gtcggcaatg tggttgcccg ccagggtcag tgcgctgaaa ccggctgcct tcagcgcatc
     2221 cagattgcgc ggatcggccc cgggagccgg cacgtcggtg tgcagcacgc ttggaccgcg
     2281 ggtatgcggc acttccagat gtccgatgct caccgcggcc gcccgcgttg cggcagcgat
     2341 accggaaagc caatagtccg catccggcac gtccagtacc agatcccccg tgaataacag
     2401 gtcgcacgcg tccatcggcg ccgctcagta tataatcgtt gagaacctca tccaattaat
     2461 ttggtgctga tcggcggcga atcgccaggt ggcataataa tagttgacga gttcaaccaa
     2521 tgggtgaggg ccggcggcag cgcctttagg gatggatgcg ccgcaggtag gcgaacagct
     2581 gtgtcagctc ctcggatgag aatagccggg tgaagcgtgc ctcgtgctcg atcatggtct
     2641 gctcgagccg ccgcagctcg cgctgtccga gcgccgtcag atagatgccc tggcggcggc
     2701 gatccaccgt ggactgcttt cgaaccgccc acttctggcg ttgtagctga tcgaccagac
     2761 cgacgatggt ggctttgtcc acgtcgagct gattggcgag ttcgatctgc gcgattccag
     2821 ggttggaatc gaccagtacc atcatgctga attcggccgg gcgcaccttg acttcgccga
     2881 gcgagcgcgc gaggtcgcgc cacagcgcga tctgggcccg acgaacgttg tagcccagca
     2941 gattgggcag catcccgacg ttgataggta ccggggctat ggcgagtgcc tgtttgcgtc
     3001 tgcgcggcga gggcttcaag gcctgctctc gcggcatctt tgctccggcc ataacattgc
     3061 gggtcaacct cggcggcagt gtaccgcaac gcccgccgac tatcgaaggc ggctgacggc
     3121 ccagcaggat gtcatgggtc ggtattcacc gctttgcgat ccagctcgcc atcttctcca
     3181 gcgccagcgg tgagagggtt tcttcgatgc gtgcatattc gctgggcgag ccggttgtgg
     3241 cggtctggaa cagatgattg agtccgggca gctcgtcgat ttgcgcatgc gtgttgccgg
     3301 cggcctgcag agcctggcga attgccggaa gattctggtc gggcaatacc tgccggtcga
     3361 gcgatccgtt gatggcgagc accggacagc tgacgtggcg tagcgcgctg gccggatcat
     3421 aggtgagaaa atagcgatac cagggggacg tgatcgcctt gagttgcatg ccgatctgcg
     3481 cctcggggat ctcgcccgcc actttttcgc gcagctgctt ttccaggacg gcttcgtcct
     3541 tttcggtttc gaccagtttc aggatgtcgc gttccttggc ggccgccttc gcggcctctt
     3601 cggcgctctt gccgccggcc agcgcaattg cctccacctg cgccggaatg acctggtcgc
     3661 cggggacacc ggtgcctgcc atcatcacga tgaaggcgac gtctttgcgg cgcgcggcga
     3721 tcatgggggc aatcgtgccg ccctcgctgt gtccgatcag tccgatctta tgagggttaa
     3781 cctcaacgcg cgttttcagg taggcgatgc cggcctcggc gtccgtggcg aaatcagcgg
     3841 tcgttgctgt ggagaaagtg ccgctggatt ttcctacgcc acgatcatcg gcgcgcagca
     3901 ccgcgatgcc gtgccgcgtc aggtaatcgg acaggatcag aaacggcttg tggcccagca
     3961 agctctcatc gcggtcctgc gggccggatc cgctgatgag cagcacggcg gtaaacggac
     4021 ccttgccctg cgggatcgtg agcgttgcgg ccagcgtgac gttctgcgcc ttgttctcgt
     4081 acgtgagctc ttcctcgtga tacggatacg gcctgacggg aacctgtggg tgcttgatga
     4141 cgagggcgct cttatccgtg acgcgcgtca gcgtgagcgg cagctccaga ccttgcgacc
     4201 actggcctga gatgctgttg ccatcggccg aaaccgtacc gtcatagcta ccgcgaacgg
     4261 ctgcgacgtc caggtgaaga ctggaattct tcagtgtcgt ggaggcgacc ggaatcgcgt
     4321 tggcaccctg gtcgaggcta tcgagcgtgg actgcaggtt gccgtcggcc ccctgagtga
     4381 tgtgcagtac cagtcgcagc ttgccggcac cggtgtcgag ggatccttgc cagtccccgg
     4441 cgatctgctg cgcgctggcg agactcgcgg tcgccatgag gcagaagagg gtcgtggcca
     4501 gggtgagaac gcgtttcatg ggcttgcctc aagaagcttc aaaagcttca aaagcgtgga
     4561 agtacgccga cttcggcaat cttgccgtcg gcattcacgc taatgatcca ttgcgactgt
     4621 tggccaccct cgtgggtgac cacgtacatg tccataccct ggggggagac gcctttgaac
     4681 tcgaccgagg cgatcgggcc gagtctggcg aattggccct ggaacaacgg caattgccgg
     4741 cgcgtcacgg actggatcag gggtgccatc tcatcgtagt cgggcgtacc actctcgagc
     4801 ccctgaatgt atttgcgcag cgccgcttcg gatccctgct gcggctgctg ggccgcggat
     4861 cgcgcctcca gttgctgcgc gaggcggctg gcagcggcct catccacgcg cagcaccacc
     4921 acgtcctcac cggcttgatg aatgctcgcg ctcatcgacg gcccggtccc ggtgctgtcg
     4981 aacgtcatct gaatatccgc gatcttccag aagaacttac tggcggtctc cggatagacc
     5041 ggcagcttgg gctgaccgct gacctgcacg ctcaactgcg cgccgtcgcg cgtcacgctc
     5101 agcaggcgct gaccgagttg atagttgccg acgtagcgat cgagcgtcgt ggcatcgacg
     5161 ctgatctgct gctgactggc cggagcttcc ggcgccgtga cctgggtggc ggcggctgcg
     5221 acgctcaggg cgagtattga ggcgagcgcg aacccgtagg tccagcgcct gtgagtgcgt
     5281 gcggtcatga cctctatcct ccgttccaac aatgtgcgcg actcggccat cgcggccaca
     5341 gcgccgatga aggcactgcg ttgctgaccc actgcaatca gggcctcacc gtaggcctgc
     5401 aagtcatgtc cggcgtccag aacacgggcg tcacagtcca cctccagcgc atgtcgcagc
     5461 cgatagagct gccaccagag cgggacgttc c
//
LOCUS       DATMRM010000302         2345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2339694, whole genome shotgun sequence.
ACCESSION   DATMRM010000302 DATMRM010000000
VERSION     DATMRM010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339694"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2345
                     /locus_tag="VNZ06_08960"
     CDS             <1..>2345
                     /locus_tag="VNZ06_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWW20920.1"
                     /translation="SEVIGQPIVRLLPELPLHGSVEQGLRALASRVDKDRGDLRPREF
                     SARHKDGHQFPAELVASKVRIEHREVFVICLRDTSERVFAEQALRDSEARYRTLVESA
                     PELIVLIDRETGHFVDANENALRFFGVSREKLPDLRPKDFSVVMQARGMDASQLFTAR
                     CERAAAGEPQVFEWLYHDSQGDEVETEVRLMALPGASGLLRASITDVTARRRAEKITA
                     GERNVFERIAADEGLAEVLDSIVELIESINGSFTAAISRLGPEGQSFVEVVGRRLPAR
                     WRAVEERMPIDIRNGSSAAAVYLGRPVLVGDIEIDAFWQRRRELALEAGFRASWAVPI
                     KSAAGRVLGSVTVFRPVVGTPSPRDLDLMVHAARLAGVAIERRRSEAALRASEAKFRR
                     LYERVLEGVYQCAPDGRILEVNPALVKMLGYGSAEEIYALPGIAMLYRNPSERAEFER
                     ALESHGEIHNAEFVLRCRDGSQVVVLESARVVRDDEGRIAAYDGTVANITARKRAEQA
                     MFAEKERAQVTLQSIGDAVITTDREGRIDYMNPVAEQLSGWRSDEARGEKLGTVLRLL
                     DQLTHSELENPLVRCLREGQLVHFAEHSVLQNRLGQEIEIEDSAAPIRDRSGEIVGAV
                     VVFRDVTKERRLKRALSYQASHDALTGLINRREFDNRLADALQSAHDGHGPHALLYVD
                     LDQFKVVNDTCGHPAGDRLLRDVTGLLQAHVRSADTIARLGGDEFGILVRHCTAEQAA
                     RIAENIRQGIRDYRFTWEQTTSSIGASIGVVEITLETDSVA"
BASE COUNT          413 a          699 c          816 g          417 t
ORIGIN      
        1 ctccgaagtg atcggccagc ccatcgtgcg actgctaccg gagctgccgc tgcacggttc
       61 ggtcgaacag ggattgcgcg cgctggcctc gcgggtggac aaggatcgcg gcgatctgcg
      121 tccgcgcgaa ttcagtgccc gccacaagga cggtcaccag ttccccgctg agctggtcgc
      181 cagcaaggta cgcatcgagc atcgcgaagt gttcgtgatc tgtctgcgtg acaccagcga
      241 acgcgtgttc gcggaacagg ctctgcgtga cagcgaagcg cgctatcgca cgctggtgga
      301 aagcgcgccc gagctgatcg tgctgatcga ccgggagacc gggcatttcg tggatgccaa
      361 cgagaatgca ctacgctttt ttggcgtcag ccgtgagaag ttgccggacc tgaggccgaa
      421 ggatttttcg gtcgtcatgc aggcgcgagg catggatgcc tcgcagctgt tcacggcgcg
      481 ctgcgagcgt gcggccgcgg gcgagccgca ggtcttcgaa tggctgtatc acgactcaca
      541 gggtgatgag gtcgagaccg aagtgcggtt gatggcgttg ccgggcgcga gcgggctgct
      601 gcgcgcgagc atcactgatg tgaccgcacg ccgacgcgcg gaaaaaatta ccgccggcga
      661 gcgcaacgtc ttcgagcgca tcgcggcgga cgaagggctg gcggaagtac tcgactcgat
      721 cgtcgagctg atcgagtcga tcaatggcag cttcactgcc gcgatcagcc gtttgggtcc
      781 tgaaggccag agtttcgtcg aggtggtcgg ccgacgtctg cccgcccgct ggcgtgcggt
      841 cgaagagcgc atgccgatcg acatccgcaa cggctcgagc gcggcagcgg tttatctggg
      901 acgtccggtg ctcgtcggtg acatcgagat cgacgcgttc tggcagcgcc ggcgtgagct
      961 tgcgctcgag gcggggttcc gcgcttcctg ggcggttccc atcaagtcgg ctgccggtcg
     1021 cgtgcttggc tcggtcacgg tgtttcggcc ggtggtgggc acgccgtcgc cgcgcgatct
     1081 ggatctgatg gtgcacgcgg cccgtcttgc cggcgtggcg atcgagcggc gacgcagtga
     1141 agcggcactg cgtgccagtg aagcgaaatt ccggcgcctg tacgagcgtg tgctggaagg
     1201 cgtgtatcag tgcgctcccg acgggcgcat tctggaggtt aacccggcat tggtgaagat
     1261 gctcggctac ggcagcgccg aggagatcta cgcgctgccg gggatcgcga tgctctatcg
     1321 caacccttcg gaacgtgccg aattcgaacg agcgctggag agtcacggcg agattcacaa
     1381 cgccgagttc gtgctgcgct gtcgggatgg ctcgcaggtg gtagtgctgg aaagcgcccg
     1441 cgtggtgcgc gacgatgagg gccgcatcgc cgcctacgac ggtacggtcg ccaacatcac
     1501 cgcgcgcaag cgcgccgagc aggcgatgtt cgccgagaaa gagcgcgcgc aggtgacgct
     1561 gcaatcgatc ggtgatgccg tgatcacgac cgatcgggaa ggccgtatcg actatatgaa
     1621 cccggtcgcc gaacagctct cgggctggcg ctcggatgaa gcgcgcggtg agaagctcgg
     1681 caccgtgctg cgactgctgg atcagctcac acacagcgag ctcgagaatc cgctggtgcg
     1741 ctgcctgcga gagggccagc tggtgcattt tgccgagcac agcgtgctgc agaatcgcct
     1801 cggccaggaa atcgagatcg aggattcggc cgcccccatc cgtgatcgca gcggcgaaat
     1861 cgtcggcgcc gtcgtggtgt ttcgcgacgt caccaaggag cggcgcctga agcgcgcgct
     1921 gtcctatcag gccagtcacg atgcgctgac cggactcatt aatcggcgcg agtttgacaa
     1981 tcgtctggcc gatgcattgc agagtgcgca cgacggtcac ggtccacacg cgctgctgta
     2041 cgtggatctg gatcagttca aggtcgtcaa cgatacctgc ggtcatccgg ccggagaccg
     2101 gctgctgcgc gatgtgaccg gcctgttgca ggcgcacgtg cgcagtgccg acaccattgc
     2161 gcgcctgggt ggggacgaat tcggaatcct ggtgcgtcac tgcacggccg aacaggctgc
     2221 gcgcatcgcc gagaacatcc gtcagggcat ccgcgactat cgcttcactt gggaacagac
     2281 caccagcagt atcggtgcca gcattggcgt ggttgaaatc acgctcgaaa ccgacagcgt
     2341 tgcca
//
LOCUS       DATMRM010000303         6598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_802602, whole genome shotgun sequence.
ACCESSION   DATMRM010000303 DATMRM010000000
VERSION     DATMRM010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802602"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VNZ06_08965"
     CDS             complement(<1..514)
                     /locus_tag="VNZ06_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
                     /protein_id="HWW20921.1"
                     /translation="MTNTVTQIANRPDEPDIRELMQVLGRAATAAAAQLALADTDRKN
                     RALRAAACALRAQHSAILAANADDVREAQAAGLTAALLDRLALDAQRVEAMAAGLDAI
                     AALADPIGGIIAQWSRPNGLRISRVRVPLGVIGIIYESRPNVTADAGGLCLKSGNASI
                     LRGGSESWRSS"
     gene            complement(511..1734)
                     /gene="proB"
                     /locus_tag="VNZ06_08970"
     CDS             complement(511..1734)
                     /gene="proB"
                     /locus_tag="VNZ06_08970"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HWW20922.1"
                     /translation="MSDRRANRPPSPLATAKRIVVKVGSALLVDAGTGRLNRAWLQTL
                     VEDLLRLRRRGAQVILVSSGAIALGRRHLNLPAGTLRLEESQAAAAVGQIRLAHAYKE
                     LLEESEVTVAQVLLTLEDSEQRTRYLNARATLESLLSLNAMPVINENDTVATAEIRYG
                     DNDRLAARVAQMASADCLVLLSDVDGLYTADPNLEPTAQFIEQVLSITPEIEAMAGRS
                     ASAVGSGGMATKIAAARIAVHAGCHMCIASGHQRHPIRRIETGARSTWFVPHSTPVAA
                     RKQWIAGTLRPAGAVRIDAGASRALQAGKSLLPAGVTGTLGRFEVGDTISVLTADGAE
                     IARGIAAYSDSDATRILGRRSGEIEAILGYRGRDELIHRDDLVILTHDRSSLDSPAVP
                     LGMAAAAAAAAEVKS"
     gene            1856..2191
                     /locus_tag="VNZ06_08975"
     CDS             1856..2191
                     /locus_tag="VNZ06_08975"
                     /inference="COORDINATES: protein motif:HMM:NF042799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6763 family protein"
                     /protein_id="HWW20923.1"
                     /translation="MAREYEPTAGQWYENLEENESFRVLSVDEDAETVEIEYLDGDIE
                     EIDLDTWAELDLDKIDEPEGWAGGDDEDDDDDDDDEEDEDLDDTWDDDDDDEDDDEWD
                     DDDEENDNY"
     gene            2241..3851
                     /locus_tag="VNZ06_08980"
     CDS             2241..3851
                     /locus_tag="VNZ06_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWW20924.1"
                     /translation="MNPPSLRRDVGALPLMLTGLGSIIGSGWLFGAWRAAGLAGPGAI
                     WAWILGALIIIMIALTYAELGAMFPESGGMVRYGHYSHGSLVGFIAAWSNWIAIVSVI
                     PVEAEASVQYMASWPYPWAHDLYVRIPGAAHGELASAGLQIAAALVIVYFLLNFWSVR
                     LFAHSNAVITLIKLVVPAATGIALIASGFHAGNLSLPAQADSAAIDFAAILNAVATAG
                     IVFSFNGFQSPINLAGEARNPGRNIPIAVLGSILLATVIYVLLQVAYLGAVPPDLLAK
                     SGWHGIDFRSPFAELAILLNLNWLAILLYADAFVSPSGTGITYTATTARMLYGMQRNG
                     TLPTFFGQLHARYGVPRAAMWFDLLVSYLFLWKFRGWSSLAAVISVATIISYLTGPVS
                     AITLRRTAASMHRPLRVPLLGVVAGLGFIFATELLYWAKWPLTGEIILLVIVALPVYF
                     YYQARSGWQDFGRQLKAAWWLIFYLPSIAAVSWAGSAKFGGRNYIEWSWDLLLVAALG
                     LVFFLWGARSGWSTPAIQQAQASNADTP"
     gene            3848..5266
                     /locus_tag="VNZ06_08985"
     CDS             3848..5266
                     /locus_tag="VNZ06_08985"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWW20925.1"
                     /translation="MSDQDPVSLRGELQSLIGSDSVLTHADDMAPLMTDHRRLYQGRA
                     LAVALPRTVEEVSRVVGWCNQRRIGLVPQGGNTGYCGGATPDESGEQLVLLLRRLNRI
                     RAIDPQNFSMTAEAGCVLAHLQQAADAVDRFFPLSLGSEGSCQIGGNLATNAGGLNVV
                     RYGMTRELVLGIEAVLPDGQILSNLKSLRKDNTGYNLKSLLVGSEGTLAVITAATLKL
                     WPKMRSSATAFIAIPKPENAIALLGTLRAGAGESLSSFELIPRIAVELTMRYIEGVSD
                     PFPASYPWYLLCELTSAGAEPLPEHLEQILSDAAERGELLDAALATSERVRAAFWKLR
                     ETVPEAQRHFGPSLKHDVSVPVASLPRFIDEASAWVRAHVPDGVLVCYGHVGDGNLHF
                     NVNRAEGAETAAFLAREPDIKRAIHDLVARYEGSFSAEHGIGRLKREELQRYSSSAAL
                     SAMRAIKQSLDPNGIMNPGKLL"
     gene            complement(5241..6143)
                     /locus_tag="VNZ06_08990"
     CDS             complement(5241..6143)
                     /locus_tag="VNZ06_08990"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWW20926.1"
                     /translation="MADPRISLEQWRALRGVVDAGGYAQAAARLHKSQSAITYAVQKI
                     ESLLKVKIFELQGRKAHLTGSGQLLYARARALLEEATSLEGAARHLAQGWESELQLAI
                     EIIFPTWLLLRCLAQFAQEHPETRIELYETVLSGTVEAMTQHRVDLAISSSVPAGFVG
                     EALMAMRFLAAAHPDHPLHKLRRKLTLQDLRKHRHLVVRDSGSQRSRDIPWLSANQRW
                     TVSHKATQIHAATQGLGFAWFAEETIRNELNQGLLKPLPMREAGDRVAQLYLVYADGD
                     YIGPGARRLADIIRDEVKAACRGS"
     gene            6256..>6598
                     /locus_tag="VNZ06_08995"
     CDS             6256..>6598
                     /locus_tag="VNZ06_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWW20927.1"
                     /translation="MSTLLQINASIHSDSGESSQLAAQFARGFKRQHPEVRIVQRDLA
                     TEVVPHLSADRFAAFLSTPEKRTDAQRAVLQFSDALIAELRGADVIVIGLPMYNFGIP
                     SQLKAYFDHVAR"
BASE COUNT         1164 a         2116 c         2062 g         1256 t
ORIGIN      
        1 cgctcgagcg ccagctctcg gagccgccac gcagaatcga ggcgttgccg gatttcaggc
       61 acagccctcc cgcgtccgcg gtcacgttcg gtcggctctc atagatgatg ccgatgacgc
      121 cgagtggcac acgcacccgg ctgatccgca gcccattggg acgactccac tgcgcgatga
      181 tgccgccgat cggatcggcg agcgcggcaa tggcgtccag gcccgcggcc atcgcctcca
      241 cgcgctgcgc atcgagcgcc aggcgatcca gcagcgccgc ggtcaggccc gcggcctgcg
      301 cctctcgcac atcatcggca ttggcggcca gaatcgcgct gtgttgcgct cgcagcgcgc
      361 atgcggccgc gcgcagggct cggttcttgc gatcggtgtc cgccagcgcg agctgcgcgg
      421 cagcggcagt cgcggcccgt cccaacacct gcatcagctc gcggatatcg ggttcgtccg
      481 gacggttcgc gatctgcgtg acggtgttgg tcatgacttc acctcggctg ccgcggcggc
      541 cgcggcagcc atgcccagcg gcacggccgg actgtccaac gaagaccgat catgcgtgag
      601 aatgaccaga tcgtcacgat gaatcagctc atcgcgcccg cgatagccga gaatcgcttc
      661 gatttcgccg ctcctgcggc cgaggattcg agtcgcatcg ctgtcggaat aggcggcgat
      721 gccccgtgca atctctgcgc catccgccgt gaggacgctg atcgtgtctc cgacctcgaa
      781 gcgtcccaag gtcccggtaa cccccgccgg caacagactc ttgcccgctt gcagggctct
      841 cgacgcgcca gcatcgatgc gcaccgcacc ggccggtcgc aaggttccgg cgatccattg
      901 cttgcgtgca gcgaccggtg tcgagtgcgg tacgaaccag gtcgaacgcg ccccggtctc
      961 gatgcgacga atcggatggc gctgatgtcc ggaggcgatg cacatgtgac agccggcatg
     1021 caccgcgatg cgggccgctg caatcttggt ggccatgccg ccggagccga ctgcggaggc
     1081 cgaccgcccc gccatggcct cgatctccgg cgtgatgctc aacacctgct cgatgaactg
     1141 cgcggttggc tcaaggttcg gatcggcggt gtacagaccg tccacatcgg acaacaacac
     1201 caggcagtca gcactggcca tctgtgcgac gcgggccgct agccggtcat tgtcgccgta
     1261 gcgaatctcg gcagtagcga ccgtatcgtt ctcgttgatg accggcatcg cgttcaatga
     1321 cagcagcgat tccagcgtgg cgcgcgcgtt cagatagcgt gttcgctgct cactgtcctc
     1381 gagcgtcaac agtacctgcg cgaccgtcac ttcggattcc tcgagcaact ccttatacgc
     1441 atgcgccagc cggatctgac cgacggccgc ggccgcctga ctctcttcca gccgcagcgt
     1501 tccggcgggc agattcaggt gccgccgtcc cagcgcaatc gcgccggatg acaccaggat
     1561 gacctgtgct ccccgacgac gcagacgcaa caggtcctcg accagcgtct gaagccaggc
     1621 ccggttcagc cgtccggtac cggcgtcgac caacaacgcg gatccgactt tgacgacgat
     1681 gcgctttgcc gtggccaacg gcgagggagg tcggtttgct ctgcgatcgc tcatacgctg
     1741 aaacttctat tccgtcggac agcgtaccgc aggtgattgc ctgactgcga atggcgccct
     1801 aatatcctgc agcgtcgcac actgtgcgta tcaaccaatc ctcgagggat agtcggtggc
     1861 cagagaatac gagcccactg ccggacagtg gtatgaaaac ttagaagaaa acgagagctt
     1921 tcgcgtgttg tcggtggatg aagacgccga gaccgtggag atcgagtatc tcgatggaga
     1981 catcgaggag attgatctgg atacctgggc ggaactcgac ctcgacaaga tcgacgagcc
     2041 cgagggctgg gctggcgggg atgacgagga cgatgatgat gacgacgatg acgaggagga
     2101 cgaagacctc gacgatacct gggatgatga cgacgacgat gaagacgatg acgagtggga
     2161 cgacgacgac gaagagaacg acaactactg atcatcctat gtaattgatt cgcaaggcct
     2221 gcctgagatt ctgatcgcgc gtgaatccac cttcccttcg acgcgacgtc ggcgcgctgc
     2281 ctttgatgtt gaccggactg gggtcaatca tcggatccgg ctggctgttc ggtgcgtggc
     2341 gcgcggcggg cctggccggc cccggagcca tctgggcctg gatactgggt gcgctgatca
     2401 tcatcatgat cgcgctcact tacgccgaac tcggtgcgat gtttcccgag tccgggggaa
     2461 tggtccgcta cggtcactat tcccatggct cgctggtggg tttcatagcg gcctggtcca
     2521 actggatcgc catcgtctcg gtgatacccg tggaggcgga agcctcggtt cagtacatgg
     2581 cgtcatggcc ctatccatgg gcgcacgatc tgtacgtgcg cattcccggt gcggcgcacg
     2641 gtgagctcgc gtcggccgga ctgcagatcg ccgctgcgct ggtcatcgtc tacttcctgt
     2701 tgaatttctg gagcgtgcgg ctgttcgcgc actccaatgc ggtgatcacc ctcatcaagc
     2761 tggtggttcc ggcggcgacc ggtatcgcac tgatcgcctc cgggtttcat gcgggaaatc
     2821 tgagcctccc ggcacaggcc gactcggcag cgatcgattt cgcggcgata ctcaatgcgg
     2881 tcgcgaccgc cggcatcgtc ttcagcttca acggttttca gagcccgatc aatctcgcgg
     2941 gcgaagcgcg caaccccgga cgcaacatac cgatcgcggt gcttggctcg attctgctgg
     3001 cgacggtcat ttacgtgctg ctgcaggtgg cctacctggg ggctgtgccg cccgacctgc
     3061 tggccaagtc cggctggcac ggaatcgatt tccgctcgcc cttcgcagag ctcgcgatcc
     3121 tcctcaatct gaactggctg gcgatactgt tgtatgccga tgccttcgtg agccccagcg
     3181 gtaccggcat cacctatacc gcaaccaccg cgcgcatgct ctacggcatg cagcgcaacg
     3241 gcacactccc caccttcttc ggccagctgc acgcgcgcta cggggtacca agggccgcga
     3301 tgtggttcga tctgctcgtg tcctatctgt ttttgtggaa atttcgaggc tggagttcgc
     3361 tggccgcggt gatctcagtg gcgaccatca tctcgtatct gaccgggccg gtgagcgcga
     3421 taaccttgcg acgcacggcg gccagcatgc accggcctct gcgcgtaccg ttgctgggag
     3481 tcgtcgccgg cctcgggttc atcttcgcca ctgaacttct ttattgggcg aaatggccgc
     3541 tcactggcga gatcatcctg ctggtgatcg tcgccctgcc ggtctatttc tactaccagg
     3601 cgcgcagtgg ctggcaggac ttcgggcgcc agttgaaagc cgcctggtgg ctcatcttct
     3661 atctgccttc gattgccgcc gtctcatggg caggtagcgc caaattcggc ggtcgcaact
     3721 atatcgagtg gagctgggat ctgctgctgg tcgcggcact cgggctggta ttcttcctgt
     3781 ggggcgcgcg ttcaggatgg agcacgccag cgatccaaca ggcgcaggcc agcaacgccg
     3841 atacaccatg agcgaccagg atcccgtgtc actgcgtggc gagctgcagt cgttgattgg
     3901 cagcgacagc gtgctcactc acgccgacga tatggcgccg ttgatgacgg atcatcgccg
     3961 gctgtatcag gggcgcgcgt tggcggtggc actgccccgc acggtcgagg aggtgtcgcg
     4021 cgtggtcggc tggtgcaacc agcgacgcat cggcctcgtt ccacagggtg gcaacaccgg
     4081 ctactgcggc ggagccacgc ccgatgaatc gggcgagcag ctggtgctgc tgttgcggcg
     4141 gctgaatcgc atccgtgcga tcgatccgca gaatttctcg atgaccgccg aggccggctg
     4201 tgtgctcgct catctgcagc aggccgccga tgcggtcgat cgtttctttc cgctgagtct
     4261 cggctccgaa ggcagctgcc agatcggtgg caaccttgcg accaacgctg gcggcctcaa
     4321 tgtcgtgcgc tatggcatga cgcgcgagct ggtactcggc attgaagcgg tactgcccga
     4381 tgggcagatc ctgtccaacc tgaagtcact gcgcaaggac aacaccggtt acaacctgaa
     4441 gtcgctgctg gtgggctcgg aaggcacgct ggctgtgatc acggccgcga cgctgaagct
     4501 gtggccgaag atgcgcagca gtgccaccgc attcatcgcc atcccgaagc cggaaaacgc
     4561 gattgcattg ctcggtacgc tgcgcgcggg ggcgggcgag tcgctgagct cgttcgagct
     4621 catcccgcgc atcgcggtcg agctcacgat gcgctacatc gaaggtgtca gcgatccgtt
     4681 cccggcaagc tacccatggt atctgctgtg cgagctgacc tcagccggcg ctgagccgct
     4741 gccggagcac ttggagcaga ttctgtccga tgcggccgag cgcggcgagt tgctcgatgc
     4801 agcgctcgcc accagcgagc gtgtgcgcgc cgcgttctgg aaattgcgcg agacggtacc
     4861 cgaggcgcag cgacacttcg ggcccagtct caagcacgat gtgagcgttc cggtcgcctc
     4921 actgccgcga ttcatcgatg aggcctcggc ctgggtgcgc gctcatgtgc ccgacggcgt
     4981 actcgtgtgt tacggacatg tcggcgacgg caacctgcat ttcaacgtca accgcgccga
     5041 aggcgccgag accgctgcgt tcctggcgcg cgaaccggac atcaaacgcg ccatccacga
     5101 tctggtcgct cgctacgagg gcagtttcag cgccgagcac ggcatcggcc ggctcaaacg
     5161 cgaagaattg cagcgctaca gctcatcggc ggctttgagt gcgatgcgcg cgatcaaaca
     5221 atcgctggac cccaacggga tcatgaaccc cggcaagctg ctttgacctc atcgcggatg
     5281 atgtccgcca gacgccgggc tcctgggccg atgtagtcgc cgtcggcata caccagatac
     5341 agctgtgcga cccgatcacc cgcttcccgc atcggcaggg gtttcagcaa tccctggttg
     5401 agctcattgc ggatcgtctc ctcggcaaac catgcaaacc ccagcccctg cgtggccgcg
     5461 tggatctggg tcgccttgtg actgacggtc cagcgctggt tggccgatag ccacgggatg
     5521 tcacggctgc gttgactgcc cgaatcgcgc accaccaggt gacgatgctt gcgcagatcc
     5581 tgcagcgtga gcttgcgcct gagcttgtgc aacggatgat ccgggtgcgc cgccgccaga
     5641 aaacgcatcg ccatcagcgc ctcgccgacg aatccggccg gcaccgagga gctgatcgcc
     5701 agatccacgc gatgctgcgt catcgcctcg accgtgccgc tgagcacggt ctcgtacagc
     5761 tcgatgcgcg tttccggatg ctcctgcgcg aattgcgcca gacaacgcag cagcaaccag
     5821 gtcggaaaaa tgatctcgat cgccagttgc agctcgcttt cccagccctg ggcgagatgg
     5881 cgggcggcac cctcgagtga ggtcgcttcc tccagcagcg ccctggcacg cgcatagagc
     5941 aactgaccac tgccggtgag gtgcgctttg cggccctgca gttcgaagat cttcaccttc
     6001 agcaatgact cgatcttctg caccgcgtag gtgatggccg actggctctt gtgcagccgc
     6061 gcggcggcct gcgcatagcc gccggcgtcg accacacctc gcagggcgcg ccactgctca
     6121 agcgaaatac gggggtcggc cataaactat ctaaccattc gattgatctg gccggattat
     6181 tgtacttttt tatcgattca acaaattgtt tactgtcgca acggacaggt tcgaggcaca
     6241 ccaggagaaa acgccatgag cacgctatta cagatcaatg ccagcattca ctccgatagc
     6301 ggtgagtcga gtcagctcgc ggcgcagttt gcccgaggat tcaaacgcca gcaccctgag
     6361 gtgcgaatcg tgcagcgcga cctcgccacc gaggtggtgc cacatctgag tgcggaccgc
     6421 tttgccgcct tcctgagcac gcccgagaag cgcaccgatg cgcagcgcgc ggtacttcag
     6481 ttctccgatg ccctgatcgc ggagctgcgc ggcgccgatg tcatcgtcat cggcctgccg
     6541 atgtataact tcggcatccc atcgcagctc aaggcctatt ttgatcacgt ggcccgtg
//
LOCUS       DATMRM010000304         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3251731, whole genome shotgun sequence.
ACCESSION   DATMRM010000304 DATMRM010000000
VERSION     DATMRM010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3251731"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VNZ06_09000"
     CDS             <1..691
                     /locus_tag="VNZ06_09000"
                     /inference="COORDINATES: protein motif:HMM:NF009205.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWW20928.1"
                     /translation="DFRPDRRNTMTDLRLNHPARADDPELPDVPVPPGPEPQEQGENK
                     AFTSPINELESRLLRQRKVLVFGAIDDKVARDVTGRLLALAADPGPIDVYVNSPGGHV
                     ESGDTIHDMIRFVDAVAPVRVIGTGWVASAGALIFLAGAKNRRLCLPNTRFLLHQPMG
                     GVRGPATDINIEAIEIIKMRERINRIIARETGQPLEKVERDTDRNYWMSAEEAVAYGM
                     VSRIITDLKGL"
     gene            809..2218
                     /locus_tag="VNZ06_09005"
     CDS             809..2218
                     /locus_tag="VNZ06_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HWW20929.1"
                     /translation="MAATSGDAVIEALRVENALLRAENAALLQRLADLERRLGLNSGN
                     SGKPPSSDGLKKPPRVSSLRQPSGKKTGGQKGHPGTTLRRTETPDATVDHYPEACAAC
                     GEPLTAAMATGHVARQVLDLPEPTPLIVTEHRAHDCRCLSCGTQTRAAFPEGVTAPVQ
                     YGKRIGAFVLYLLHYQLLPEKRLAALMADLFGVRLVTATIARISQDCAHRFMGFADAV
                     RDHVAAAPVKHLDETGFRTGGKTQWLHIASTVWLTFYRVSARRGSLLPKVTGIVVHDH
                     WKPYYTMTGVLHALCNAHHLRELKALVEIEKEDWARKMQRLLRRACHAANLAHQQGVP
                     LKAGLIALLDRCYDTILAEGLAFHQAQPALAKVRRRGRQPRRVGHNLLLRLSTRRLDV
                     LRFLTDPSVPFTNNLAEQDGRMMKLRQKISGGFRSEDGAKDFAVIRSVLSTARKQGWN
                     MLQTLTTAPTRLITDIRLA"
BASE COUNT          455 a          763 c          762 g          391 t
ORIGIN      
        1 agatttccgg cctgaccgga ggaacaccat gaccgatctc cgattgaacc atccggcccg
       61 cgctgacgat ccagaattgc cggacgtccc cgtgccgccg ggtccggaac cgcaggagca
      121 gggcgagaac aaggccttca cgtcgccaat aaacgagctg gaaagccggc tgctgcggca
      181 gcgcaaggtg ctggtattcg gcgccatcga cgacaaggtg gcgcgcgacg tgaccgggcg
      241 cctgctcgcg ctcgcggccg acccggggcc aattgacgtc tacgtcaact cgccgggcgg
      301 ccatgtcgag agcggcgata ccatccacga catgatccgc ttcgtggatg ccgtcgcacc
      361 ggtgcgggtg attggcaccg gctgggtggc gagcgccggt gcactgatct ttctggccgg
      421 cgccaagaat cggcgcctct gcctgccgaa tacccgtttc ctgcttcacc agccgatggg
      481 cggggtgcgc ggcccggcga ctgacatcaa catcgaggcg atcgagatca tcaagatgcg
      541 ggaacgcatc aaccgcatca tcgcccgcga gactggtcag ccgctggaaa aggtcgagcg
      601 cgacactgac cgcaattact ggatgagcgc ggaggaagcg gttgcctacg ggatggtgag
      661 ccgaatcatc acagatctga aggggctata ggctcggacc gtaactgccc aggtcggtct
      721 ctgatgggga ccattgacag gtcacgggcg agtacgagtc aacgtgtcga tggcagcgcc
      781 cgacgacacc ccgtcacgag acgaccgtat ggccgcgaca agtggcgatg cggtgataga
      841 ggcattgcgg gtcgagaatg cgttgcttcg tgcggagaat gctgcactgt tacagcgact
      901 ggcggacctg gagcgccggc tcgggctgaa cagcggcaac agcggcaagc cgccgtccag
      961 cgacgggctg aagaagccgc cgcgcgtcag cagtctgcgg caaccgtcag gcaagaaaac
     1021 cggtggtcag aaaggccatc cgggcacgac gcttcgccgg accgaaaccc ctgatgccac
     1081 cgtcgaccat tatccagaag cgtgcgcggc gtgcggtgaa ccgctgactg cggccatggc
     1141 aaccggccat gttgcccggc aagtgctcga tctccccgag ccgacgccac tgatcgtcac
     1201 tgaacaccgc gcacatgact gccgctgcct cagctgcggc acgcagacgc gggcggcgtt
     1261 ccccgagggg gtgaccgctc cggtgcagta cggcaaacgg atcggcgcgt ttgtgctgta
     1321 tctgctgcac tatcagttgc tgccggaaaa gcgccttgcc gcactgatgg ccgacctgtt
     1381 cggcgtcagg ctggtcaccg cgaccatcgc ccggatcagc caggattgtg cgcaccgctt
     1441 catgggtttt gccgatgccg tgcgcgacca cgtggcagcg gcaccggtca agcacctgga
     1501 tgagaccggc ttccgcactg gcggcaagac gcagtggctg cacatagcct cgactgtctg
     1561 gctcaccttc tatcgcgtct cggcgaggcg cggcagcctg ttgccaaagg tcaccggcat
     1621 tgtcgtgcat gatcactgga agccgtacta cacgatgact ggcgtgctgc acgcgctgtg
     1681 caatgcgcac catctgcgcg aactgaaggc gctggtcgag atcgagaagg aagactgggc
     1741 gcgcaagatg caacgcctgt tgcgtcgcgc ctgccacgcg gccaaccttg cgcaccaaca
     1801 gggcgtgcca ctgaaggcgg gcctgatcgc cttgttggat cgatgttacg acacgatcct
     1861 cgcggaaggt ctggcgttcc accaggcaca gcccgcactg gcgaaagtcc gacgccgtgg
     1921 ccgtcagccg cgacgggttg gccataatct gctgctgcgc ctcagcacgc gtcggctgga
     1981 cgtgctgcgc ttcctgaccg atcccagcgt gccgttcacc aacaacctcg cggagcagga
     2041 tgggcgcatg atgaaactgc gccagaagat ctccggcgga tttcgctccg aggatggtgc
     2101 gaaggatttc gccgtcatcc gctcggttct ctccactgcc agaaagcagg gttggaatat
     2161 gctgcagacc ttgaccactg ccccaacgcg cctgatcaca gacatccggc tggcttgatc
     2221 cgcctcttcg ctacctgggc tgttaccccc cgacgcgatt ccctcgtgca agctgatcga
     2281 ccaccgcgcc gattcgcggt gtgccgcaag gagcctgccg aacccagaac ccagaaatgc
     2341 caaagcccgc cggtgagggc gggccatgaa c
//
LOCUS       DATMRM010000305        18325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3252114, whole genome shotgun sequence.
ACCESSION   DATMRM010000305 DATMRM010000000
VERSION     DATMRM010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18325
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3252114"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VNZ06_09010"
     CDS             <1..671
                     /locus_tag="VNZ06_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWW20930.1"
                     /translation="RYNGVEHLVYASTSSVYGASTKMPFTVHEPASHPLSLYAASKRS
                     TELMAHNYSALFKLPTTGLRFFTVYGPWGRPDMALFLFARAIIEGRPIEVFNHGHHTR
                     DFTYVEDIAEGVVRATDRVARANSAWDSSNPDPASSSAPYRLYNIGSNRPIQLLRYIE
                     VLEDCLGRKAERVLKPLQLGDVPDTFANVDELITDVGYKPDTPIEVGVRNFVNWFKHY
                     YHYD"
     gene            675..1778
                     /locus_tag="VNZ06_09015"
     CDS             675..1778
                     /locus_tag="VNZ06_09015"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWW20931.1"
                     /translation="MDFIDLRSQYRALRGEIDARIARVLEHGQFILGPEVSELETRLA
                     AYVGTQHCAAVASGTDALLIALMALGIGPGDEVITTPFTFVATLEVVVLVGAKPVLVD
                     VEADTGNLDATQLERALTERTRAIIPVGLYGQPADMDAISQIAAKHGNTAVIEDAAQS
                     FGAEYRGRRSCNLSSIGCTSFFPSKPLGCYGDGGAIFTSDAAIDRACREIRVHGQSAR
                     YHHTRIGIAGRMDTIQCAVLLAKLERFPWELQRRAELARRYDERIRAAGVRAQRLSSR
                     PDRRSAWAQYTVLVDDRDGVQQRMQRLGVPTAVHYPRSMHRQPAYERQCKPGDYPVSD
                     DLASRVISLPFSADLTEEQLESVVQALRQATGS"
     gene            2028..3434
                     /locus_tag="VNZ06_09020"
     CDS             2028..3434
                     /locus_tag="VNZ06_09020"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HWW20932.1"
                     /translation="MSDAVVFKKSLAIALMSVLNAILPTTCVVACLYFCETGYGEPLN
                     TDFLVLELVVAVLSLVLLQPRGELTDRLSSGPAAMSISVALRWLILLSILFFIGYITK
                     SSAIYARRAILTWAVLTPCLLAIISLVIFAMLRRVVMDPANQRRAIIAGANEISATLC
                     LRLQEHSELGMSVSGFFEDRSPERVDVKCPAPILGRLPDLAAYVKAHQIDVIFVALPI
                     RHVHRVLNLIDELRDTTASIYYVPDIFVFDLIQARTMDIVGIPVVAMCETPFAGYRGV
                     VKRSMDIVLCLMALPILLPIMLVLGLLVAVSSPGPIIFRQRRYGLDGEQIIVYKFRTM
                     TVLEDGSKVTQATKDDTRVTPIGRLLRRYSLDELPQLINVVQGRMSLVGPRPHAVAHN
                     EEYRKLIKGYMVRHKVRPGITGLAQVNGCRGETSRLEDMQARVHHDLEYLRHWSALLD
                     LQIIAKTLLQLVSNEKAY"
     gene            3431..5389
                     /locus_tag="VNZ06_09025"
     CDS             3431..5389
                     /locus_tag="VNZ06_09025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWW20933.1"
                     /translation="MNSRADPSPASIVDRLLGRTAEISASLFAVRPPDQSGHRWPVLG
                     LIVVMIVGAWLRFWGLATWGLAGDEKTMALPTLHILQFGTPHMPAGMLYPRAVAQLYM
                     MAASASIFGLSEWAFRVPSVLCGIALIALAYPLGRRFLSPSWNFAFVVTVALLPALIA
                     DSQEARMYIFMVACLAGYTELVFRWESTGRTGYLLAAVLVMLLSLQFHVLSVFAGFLL
                     LFPGLVRGDARKLWLGALALAFVGCCFALLNRWIDSCYPPRMHSGLARTEGASHLQLL
                     RSGLGFAFALLEVALAAGIAWLTARKVPTRGIGLLVGCLVFAGLVAELGLHFHLGAVL
                     LLIALVVAKRQGMRILPYALLIVLLTAVVAGAEFAWLHTLTGDPARKIIGALLGEPSF
                     WSFLRAATYSPLAWSVVFLGLVYALWQVAQRRAVPDYWLFFVLAVWLPLFGLGFFSWY
                     FEVRYTEFALLPVLLVTFALLQQVFSRATTRAAGAPLVLALIAAALIINPLELIHSVG
                     PNPATHPDHKGAAAYIRSLHLAPNDILVAEDTLEQTYYLGHVDYWLHGRFDALQFVQS
                     KHGELRDIYTDTPLIGTGDELQALIQRHDRGTIYVIGSGEDYIDDRRYMRSDGIYEVM
                     QQPEFQQIFLGSDRTTRVWRIAPLPTRD"
     gene            5393..6844
                     /locus_tag="VNZ06_09030"
     CDS             5393..6844
                     /locus_tag="VNZ06_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWW20934.1"
                     /translation="MAGFARKAIVLTISRLANYALMIVSPVVLVRMLTKEDFGHYRNF
                     LLYASILQAVATFAINDSLLYFIPTHPKSPWRLVRETNVLTLGLSVAVLLVVLAANAL
                     SSGHLIGEYPLPVCIYVLLYANIDFWECYWLAEHRPNPVFAYTMARLMTRMLVVTSVA
                     ILTHDVWKIVLALIALEAVRMGMSIAAWGSFSQAAREPKVENIRREQLRFCVPAGLAT
                     ICYMISRNLGNIAVTRYLGDAALGVLTVGTFGEPIIMALRNSISTIVLPELVRRSAQD
                     APGSLLLWQRAVVVNCILLLPSAALVALFAEPLVLTAFKEAYAAAIPVMQIYALVIVR
                     SCFDFAPPLRAVNKTGPLMTSNFIAGIGSGVTLLLLLPVDGIVGAGWALVVSNMLEAI
                     YLAWSVKRHYKLTYASMLPWNVVLHVALCAGLSALPILAIEQYTQVNIIGAMLLGGVY
                     VAVFALLLLALRVEEAIKLGRRIKFSVLSFARS"
     gene            complement(6739..7965)
                     /locus_tag="VNZ06_09035"
     CDS             complement(6739..7965)
                     /locus_tag="VNZ06_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20935.1"
                     /translation="MPARALRSMVTHCRLVMWSRAAHLSQLLTGFAMLARRGAIRLTQ
                     ELQDIDYERPEAPSHLRHARREHLLVIVNHSLRVYFDTHDGNEIDPDAIRDCDMYFKR
                     SFSPSAVPSQFRHKVAPLGLNYEVYGDHFDLHELRRMLAFPAYATPRGKEFAAYLMRI
                     AGLHFGIGMQLPVSDMQAAPRLDARARVLFIAKAWDPAGVAGFSPRKAEERETMNEMR
                     AECISAMRARLGDRFLGGFQHDEFAARRYPKQLLQDSALSRKRTYLRLVRDSAICVTT
                     TGLHGSMGWKLAEYVAFSKAILSEQLLYKVTGDFGEWNNYLSFSSSGECAERAQQLLG
                     DLSLRRRLMNNNWTYYNAYLRPDALVGRAVNAALERRAVTQDLANDSTENLILRPSLM
                     ASSTRRASKSSAKTAT"
     gene            8209..9552
                     /locus_tag="VNZ06_09040"
     CDS             8209..9552
                     /locus_tag="VNZ06_09040"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWW20936.1"
                     /translation="MAYLLTLPILVYVIGATFGMPLSFGLGLSLKNGVVYLVGLMLCM
                     RIAIRGNYKFEQPLIFTCFAIMIIYAIVTWLMAGLVIHYDAYQIVPSAISLKGSLIDN
                     AIIFTLFFFGVRDAKDCILLTKVLVAVVALSNILCILSIEGIVYIHGVAATPGQEGRV
                     QGVYGDANETAALVAGMLPAFVAMVLSDGAAWALLWIVCAIGSIAMLFMTVSRGAFVA
                     MGIAYPVCIYTFRRYIAIRQVFIWAGALVGLGVVALAAAGPRFVTLFLTRFINESDVS
                     SLDYISSGRSAIWARAIGKMMSYPITLFTGFGWNVYDTMGFFYVPHNSYLMMWFELGL
                     VGLLSFLLILFRIVTTTRRAAEVADPVSRTYLIAFVFGFAAFLVAIFFVNTYLTWAYM
                     WMYAALSLRLALCVAQAPQRSRPAGAGTGRVLRRSRRKGVAEPGSGRARPVAGAS"
     gene            complement(9596..12514)
                     /locus_tag="VNZ06_09045"
     CDS             complement(9596..12514)
                     /locus_tag="VNZ06_09045"
                     /inference="COORDINATES: protein motif:HMM:NF025264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HWW20937.1"
                     /translation="MLKPTSCSALTLLVVCAGLLTGCGANSSPWGAQLEGTNGPTAPS
                     TALQAPQIVQQPQNATVSDGSPVSFSVVASGTGALVYQWLINGQGMPGGTGSTLVMRG
                     AALAQTGSLLSVIVRNTAGSATSRSATLTVTAVAPSISTQPTSQTVVPGATASFSVVA
                     KGSAPLAFQWNRNGVVIAGANTANYVTPAASLSDSGSIYTVTVSNGVGSVTSNNATLT
                     VSPVIVAPTIVTPPQNVTVPFGGTASFSVTASGTQPLTYQWTRNAANIAGATGTSYTV
                     TPALPQDNGAQFAVVVTNAAGSATSQPATLTVSGPAVGVALLAGQLGGSGSIDGAGAG
                     ARFYAPAGIATDAAGNVYVADSTRDIIRQVSPNGVVTTIAGTAEIALSSDGIGTNAYF
                     TNPGGVAVDPSGNLYVADTGNDTIRLIQPNGTVSTIAGTAGNVGNSDGTGNTASFNAP
                     GGIATDAAGNVYIADTGNNTIRKMTPAGAVSTLAGTAGVVGSQDGTGAGARFNAPEGL
                     TVDAAGNVFVADTLNNTIREITPTGVVTTIAGTAGVFGLSDGPGASAQFGNVYGIAID
                     ASGNLYVSDLGGMTIRMISAAPQFVVSTIAGNYLRGTVNGPEAQAEFNVPRGVALDGG
                     GNIYVSDYGDNTIRKLSSGLVTTLAGLQGQPGSTDATGPAASFNGPLLATCDNAGNVY
                     VADVNNNTIRKITLGGVATTLAGTAGVTGSADGSASSASFNAPVGIVFDGMSALYVTD
                     SGNNTIRKVTLDGVVSTVAGSVGTTGSVDGNGSAASFNGPTGIATDGQSNFYVTDTGN
                     NTIRRVTAAGAVATIAGTAGVTGTLDGAGAAAQFNAPQGIASDGAGNLYVADTGNSTI
                     RAITQAGVVTTLAGNPASPGPGDGVGSAANFLYPEDITLDNGVLYVSDTVNMSIRQVT
                     TAGAVTTVVGAPRSEGVILGALPGSLNNPYGIASIPGVPKSLIVTTAVENSILLVTFP
                     "
     gene            12877..13839
                     /locus_tag="VNZ06_09050"
     CDS             12877..13839
                     /locus_tag="VNZ06_09050"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWW20938.1"
                     /translation="MSQVSLITLTKGIATFVPGVNKLATRRTSGTSSARYCYSVWMRH
                     LAAARAVGAPWPVKAVAELGPGDSIGSGLSAILSGAQSYIGLDGFAFAAASSTLEIFE
                     ELVSLFKARAPIPNPDEFPDIWPRVDSYDFPHDLLTESLLSEALDPSRLEAIRKALRG
                     GLKSDSGQDGAGVTVRYAAPWYAKEVMRENSVDMIFSQAVMEHVDNVIETYQSARRWI
                     KTGGFMSQTIDYSSHRFTRDWYGHWTLSRPMWRMVAGRRKYTINRWPHSAHLKAMETT
                     GFRIIGESVMRDAALERAHLAPEFRDLTDVDLAAHAAFIQTLAV"
     gene            complement(13849..15423)
                     /locus_tag="VNZ06_09055"
     CDS             complement(13849..15423)
                     /locus_tag="VNZ06_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20939.1"
                     /translation="MRRAAWGKLEDFKMRVTLWVALAGLSLSSASFGSPDVNSTIGYP
                     RTMVQAISGAQCNISSANYTLAARYDIVMLGCNWENQGVNIGNVVNGIHAASNGTMVS
                     PVHLVGRYSILEVPSGANLSASANEDRQVQTAGWYLCPLGTSCNNATAVNDSDGHMAL
                     VNFSTYAPSYSGQTPYQWFASYVWNTYGLGKGGPVNGANANNAAPNVDFFYFDNTLPT
                     AKFSGDWLRNGTLPGTLANTPLALDTAIQNAQLQLCPALHSASGNKLCFVNQALGWTD
                     SLGVNYASGVGAFDGAMQQFIWGTGLSNAVETWGGFTYAMNEYKSQVAGGAPYVMVTG
                     PVANSTAADYQYFRYAFGMCTMDIGYCTFGLTSYYPSADSIDGSNTTTWPWFDEEQGG
                     TLKNVKWLGLPANPNNVSNSCGVPVSNTWTNGNTCAWKNGVYRRDFQNGIILVLPRQT
                     TGGAAVTVTTEVSYCHLQNSNDTVDASVNNGSCGTSFTLNPSAITSTGDALFLSRTPV
                     NSIKPNPPSNISASPH"
     gene            complement(15750..16892)
                     /locus_tag="VNZ06_09060"
     CDS             complement(15750..16892)
                     /locus_tag="VNZ06_09060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWW20940.1"
                     /translation="MGIGNLPALAPEYGHLGNGGAEVQQSVLAKALAARDFKVSMVVA
                     DHGQPDGAVWGGVTTYRAYRLNAGLPGLRFFHPRWSSVWAALRRADADIYYVGCAGVL
                     IWQVSLFTRMFGRKVVFGTMSDSDCDPNALLVKLWRDKKLYAQGLRRADLVLTQSALQ
                     QQMLRRNYRRDSKVVPAIGGMIGRRLTREQRDIDALWVANLRPLKRPEMFIELARELP
                     HLRFHMIGGAWDGLVDYFNSVREKAAACPNIHFHGSVSYHEVNSYFERTRVFVNTSEI
                     EGFPNTYLQAWGHGVPVVTFLDPDNLIAQNSIGRAVANLEELRTAVDAFTDDSDEWQR
                     ASTRSIDFMDRDYNPHTAVNAYVEAFTTLGIQGALESAMPLNASRK"
     gene            complement(16942..17949)
                     /locus_tag="VNZ06_09065"
     CDS             complement(16942..17949)
                     /locus_tag="VNZ06_09065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW20941.1"
                     /translation="MTQSDAEIQLSVIIPVGDRHADPQSLLAEYSAGLAAVGQRYEII
                     FVLDGPQTDFATALQDLTAEHNDISIVSLTRPFGEATALMAGFARAAGQLIINLPAYH
                     QIQGMEITKLVAGLDEADLVIARRWPRAGGSFEGLRRSAFHGLIASVTGQRFHDLGCG
                     ARVMRRRVLEEITLYGDQHRFLALLADRQGFRVREINVAQSALDRFTGHYRAREYAHR
                     VLDIFTVFFLVRFTKKPLRFFGMLGVSTFVIGGALLAYLVVDRLIFNVGLADRPALLL
                     SSLLVVLGLQLFALGLLGELIIFTHAKNIKDYQVDRVIEFTKASHSDYARRPDKSAIA
                     N"
     gene            complement(17957..>18325)
                     /locus_tag="VNZ06_09070"
     CDS             complement(17957..>18325)
                     /locus_tag="VNZ06_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20942.1"
                     /translation="HARQFGKSKYGLSRIYKVLLDLLVIKTVASFTARPLIWFTLLSL
                     PLVAIGLFALAYSGYVMVSEHRGMPLPVAGTGLIFLMSAFILVSAGAFGELVYNTGDM
                     REYDFARLTERVLKPPASGA"
BASE COUNT         3356 a         5428 c         5638 g         3903 t
ORIGIN      
        1 gccgctacaa cggggtcgag cacctggtgt acgcctcgac cagttcggtc tatggtgcca
       61 gcaccaaaat gccttttacc gtgcacgagc cggcttcgca tccgctgtct ctctatgccg
      121 ccagcaagcg ttcgaccgaa ctgatggcgc acaactactc ggcgctattc aaactgccga
      181 caacgggact gcgttttttc acggtctatg gcccgtgggg ccgacccgac atggcgctgt
      241 tcctgttcgc gcgagcgatc atcgagggcc ggcccatcga ggtgttcaat cacgggcacc
      301 acacgcgcga cttcacgtat gtggaggaca tcgctgaggg tgtggtgcgg gcgaccgatc
      361 gcgttgcgcg agcaaactcc gcttgggata gttccaaccc ggatcccgct tccagtagcg
      421 caccgtatcg gctctacaac atcggcagta accggccgat tcagctgttg cgctacatcg
      481 aggtgctcga ggactgcctt ggccgcaagg ccgagcgcgt cctgaagcct ttgcagttgg
      541 gagatgtgcc ggacacattt gccaatgtcg atgaactcat caccgacgtc ggttacaagc
      601 cagatactcc gatcgaggtt ggcgtgcgta actttgtcaa ttggttcaag cactactacc
      661 actacgacta acccatggat ttcatcgatc tgcgatcaca ataccgcgcg ctgcgcggcg
      721 aaattgacgc acgtattgcc cgcgttctag agcacggtca atttattctc ggtcccgagg
      781 tcagcgagct cgagacgcgg ctcgcggcat acgtcggtac gcagcattgc gccgctgtcg
      841 ccagcggtac ggacgccttg ttgatcgcct tgatggcgct aggcatcggg ccgggcgatg
      901 aagtcatcac gactccgttt acctttgtgg ccactcttga ggtggttgtg ctggtaggtg
      961 ccaagccggt gttggtggat gtcgaggccg acacgggcaa cctcgacgct acccaactgg
     1021 aacgggctct cacggagcgc acccgtgcga tcattccggt gggcctgtac ggacaaccgg
     1081 ctgacatgga tgcgatcagt cagattgcag caaagcatgg caacaccgcc gtcattgagg
     1141 atgccgccca aagcttcggc gccgagtacc gaggtcgccg cagctgtaac ctgtcttcca
     1201 tcggttgcac cagtttcttc ccgagtaagc cacttggatg ctacggcgac ggcggggcca
     1261 tctttaccag cgacgcggcg atcgaccgcg cctgccgcga gatccgggtc catggccaga
     1321 gcgcgcgtta tcaccatacc cgcatcggca tcgccggtcg catggatacg attcaatgcg
     1381 ccgtccttct ggcgaaactg gaacgcttcc cgtgggagct gcagcgccgc gcggaactgg
     1441 cccgccgcta cgacgaacgt attcgagcgg ccggtgtgcg cgcacagcgg ctgtcctcaa
     1501 ggcctgatcg ccgcagtgca tgggcgcaat acacggtgct ggtcgacgat cgggacggcg
     1561 tacagcagcg gatgcagaga ttgggggttc cgaccgccgt gcactaccca cggtcgatgc
     1621 accgtcagcc cgcttacgag agacagtgca agccaggcga ctatccggtc agcgatgatc
     1681 tggcgagccg ggtgatcagt ctgccgttca gtgcagacct caccgaggag caactggaat
     1741 ccgtggtcca ggcgctgcgt caggccacgg gctcatagct agcctcttgg cgcaatcttt
     1801 tcaatcagtt gcgatctgtc ggtctagagc ttggtcacag tatactgggc acgccgagac
     1861 tatagtcttt cgaccgtacg gcaaatggtg acataacgcg ctaccgacca agacccgatg
     1921 tcctctgatg ccgcgtcgac ccccagacat cctgaactta ccgcgcctgg tttcacctgg
     1981 cgctgcggca agcgttgcgt agagcttaac cagcccctgg agtagcgatg tccgatgccg
     2041 tggtgttcaa gaagagcctc gccattgcgc tgatgagcgt cttgaatgcc atcctcccga
     2101 ctacctgcgt cgttgcctgc ctgtacttct gcgaaaccgg atacggcgaa ccgctcaaca
     2161 cggattttct ggtgcttgaa ctggtggtcg cggtgctctc gctggtactg cttcagccac
     2221 gtggcgagct gaccgatcgg ctcagctccg gccccgctgc gatgtccatc agcgtggctc
     2281 tgcggtggtt gattttgctg agcattctgt tcttcatcgg ctatatcacg aagtcgtcag
     2341 caatctatgc gcgccgcgca attctcacct gggcagtgtt gacgccatgc ctgctggcga
     2401 tcatctcgtt ggtgatattc gcgatgctta gacgcgtcgt catggatcca gccaatcagc
     2461 ggcgcgcgat catcgctggc gccaatgaga tcagcgccac actgtgcctg cgtttgcagg
     2521 agcattcgga acttgggatg tcggtctcgg gtttcttcga ggaccgcagt cccgagcgcg
     2581 tcgacgtaaa gtgtccagca ccgatcctcg gcaggctccc ggatctggcg gcctatgtga
     2641 aggcacatca gatcgatgtg atcttcgttg cactgccgat ccgacatgtg caccgtgtcc
     2701 tgaatctgat cgatgagctt cgcgatacca cggcgtcgat ctactatgtg cctgacatct
     2761 tcgtgttcga cctcattcag gcgcgcacga tggacatcgt cggtataccg gtcgtcgcaa
     2821 tgtgcgagac acccttcgcc ggataccgcg gagtcgtcaa gcgctcgatg gacatcgtgc
     2881 tgtgcctgat ggcgctaccg atactgctgc ccatcatgct ggtactcgga ttgttggtcg
     2941 cagtgtcttc gcccggtccg atcatttttc gacagcgccg ctatggcctg gatggcgaac
     3001 agatcattgt gtacaagttt cgcacgatga cggtgctgga ggacggctcg aaggtgaccc
     3061 aggccacgaa ggatgacacg cgcgtgactc cgatcggcag gttgctgcga cgctattcgc
     3121 tggacgaact gccgcaactc atcaatgtcg tgcagggacg gatgagtctg gtgggcccaa
     3181 gacctcacgc ggtcgcccac aacgaggaat accgcaagct catcaagggc tacatggtca
     3241 ggcacaaagt gcgtccgggt atcaccggac tggcgcaggt caacggctgt cgcggagaaa
     3301 cctcgcggct ggaagacatg caggcacgag tgcatcacga tctagagtat cttcgtcact
     3361 ggtctgcgct gctggatctg caaatcatcg ccaagaccct cttgcagctg gtcagcaacg
     3421 agaaagccta ttgaactcgc gcgctgaccc ttcacccgcg tcgatcgtgg accgcctgct
     3481 tggccgtacc gccgagattt cagcgagcct gttcgccgtg cgccccccgg accaatccgg
     3541 tcatcgatgg ccggtgctgg gcttgattgt cgtgatgatc gtgggtgcgt ggctgcgctt
     3601 ctggggcctg gctacctggg gactcgcggg cgacgagaag accatggcgc tgccgacatt
     3661 gcacattctg caattcggaa cgccccatat gcctgcaggc atgctgtatc cccgggcggt
     3721 ggcgcagctc tacatgatgg ccgcgtcggc atcgattttc ggcctgtcgg aatgggcgtt
     3781 ccgcgtgcct tcggtgctgt gcggcatcgc tttgatcgcg ttggcctatc cgttgggtcg
     3841 gaggtttctg tcgccgtcct ggaatttcgc attcgtagtc accgtagcgc tgctgcctgc
     3901 gttgattgcc gattcccagg aagcgcgcat gtacatcttc atggtggcat gcctggcggg
     3961 ctataccgaa ttggtattca gatgggagag caccgggcga accggctacc tgctggcggc
     4021 ggtgttggtg atgctgctca gcctgcagtt tcacgtgctc tccgtgttcg ccggatttct
     4081 tctcctcttt ccgggattgg tgcgcggcga tgccaggaaa ctgtggctcg gcgctctggc
     4141 gctcgctttt gtcggctgct gcttcgcgtt gctcaatcgc tggatcgata gctgctatcc
     4201 gccgcgaatg cactctggcc ttgcacgcac ggagggtgcg tcgcatctgc aactgctgcg
     4261 atcgggactc ggattcgcct tcgcgctgct cgaagttgcg ctcgcggcgg gcatcgcgtg
     4321 gctgactgcc cggaaggtac cgactcgcgg catcgggctg ctggtgggct gcctggtgtt
     4381 tgcgggattg gttgccgagc tcggtttgca ctttcacctg ggagcggtgc ttctgctgat
     4441 cgcgttggtg gtagcgaagc ggcaaggtat gagaatcctc ccgtacgcgc tcctgattgt
     4501 gctgctgacg gccgtggtgg ccggagccga gtttgcgtgg ttgcacacgc tgaccgggga
     4561 tccagctcgc aagatcatcg gtgccttgct cggtgagccc agtttctgga gctttctgcg
     4621 cgcggcgacc tattcgccgc ttgcctggag cgtggtgttt ctcggcctcg tctatgcgct
     4681 gtggcaggtt gcgcagcggc gcgcggttcc ggactactgg ctgttctttg tgcttgcggt
     4741 ttggctacca ctgttcgggc tcggcttctt ttcctggtac ttcgaggtgc gctatacgga
     4801 gttcgcgctg ttaccggtgc tgctggtcac tttcgccctg cttcagcaag tattcagtcg
     4861 cgcaacgact cgagcggcgg gggcgccgct cgtgctcgcg ctgatcgccg ccgcactcat
     4921 catcaatccg ttggagttga tccactcggt cggcccgaat cccgcaacgc atccggatca
     4981 caagggagcg gcggcctaca tacgctcgct gcatcttgcc ccgaatgaca tcctggtggc
     5041 cgaagatacg ctggagcaga cttactatct cggtcatgtg gactattggc tgcacggtcg
     5101 attcgacgcg ctccagttcg tgcagtccaa gcacggggag ctgcgcgata tctatacaga
     5161 tacgccgctg atcggcacgg gcgacgaact ccaggcactg atccagcggc atgaccgcgg
     5221 caccatctac gtcatcggta gcggcgagga ctacatcgac gatcgtcggt atatgcgcag
     5281 cgacggcatc tacgaggtta tgcagcagcc ggagtttcag cagatctttc tgggaagcga
     5341 ccggacgacg cgagtttggc gcatcgcccc gctgccgacc cgggactgac acatggcagg
     5401 ttttgcccgc aaggccatcg ttcttacgat ttcgaggctt gctaactacg ccttgatgat
     5461 cgtgagcccg gtggtgttgg tccggatgct gaccaaggaa gacttcggcc attatcgaaa
     5521 tttcctgctg tacgcgagca ttctgcaagc ggtcgcgacg ttcgcaatca acgacagcct
     5581 gctgtatttc attcctacgc accctaagag tccctggcgc cttgtgcgcg aaaccaatgt
     5641 gttaacgctg gggcttagcg ttgccgtgct gctggtggtg ctggcggcca atgccctgag
     5701 ttccggccat ctgatcggtg aatacccgct gccagtgtgc atctacgttc ttctctacgc
     5761 gaatatcgat ttctgggaat gctattggct ggctgagcat cgtcccaatc ccgtgttcgc
     5821 atatacgatg gcgcgactga tgacacgcat gcttgtggtt accagcgtag cgatcttgac
     5881 ccacgacgtc tggaagattg tgctggcgct gatcgcgctc gaagccgttc gcatgggaat
     5941 gtcgatcgca gcatggggct cgttcagcca ggcagcgcgc gaaccgaaag tcgaaaacat
     6001 acgtcgcgag cagcttcgat tctgcgtccc ggcgggactg gcgacgatct gctacatgat
     6061 cagccgcaat ctgggcaaca tcgcggtcac ccgctacctg ggcgatgcgg ccctgggcgt
     6121 attgacggtg ggcaccttcg gcgagccgat catcatggcg ctgcgtaact ccatctcaac
     6181 catcgtgctg ccggaactgg tccgacgcag tgctcaggac gccccaggct cgttgctgct
     6241 gtggcagcgg gcggtcgtcg tcaattgcat cctgctgctg ccatcggccg ctctggttgc
     6301 gctgttcgct gagccactcg tgctgacggc gttcaaagaa gcttatgcgg cagcgattcc
     6361 agtgatgcag atctatgcgt tggtgatcgt gcgttcgtgt tttgatttcg cgccgccact
     6421 gcgcgcggtc aacaagaccg ggccgctaat gaccagcaat ttcatcgcgg gtatcggtag
     6481 cggtgtgacg cttctgttgt tgcttccggt agacggtatc gtgggcgcgg gttgggcact
     6541 ggtggtctcc aacatgctgg aggcgattta cctggcgtgg agcgtcaagc ggcattacaa
     6601 gctcacatac gcctcaatgc tgccatggaa cgtggtgctg cacgtggcgt tgtgtgccgg
     6661 cctcagcgcg ctgccaatcc tcgcgatcga gcagtacacc caggtcaaca ttatcggtgc
     6721 gatgttgctc ggcggtgtct atgtcgctgt cttcgcactg ctcttgctgg ctctgcgtgt
     6781 cgaggaagcc atcaagcttg gccgcaggat caagttctcc gtgctgtcgt tcgccagatc
     6841 ctgagtgacc gcgcgccgtt cgagcgccgc attcaccgcc cggccgacca gtgcatcggg
     6901 acgcagatag gcattgtagt aggtccagtt gttgttcatg agcctgcggc gcaggctgag
     6961 gtcgccgagt agctgctgcg cacgctcggc gcattccccg gagctcgaaa agctcaggta
     7021 gttattccat tcgccgaagt cgccggtgac tttgtacagc aattgttcgc tcaggattgc
     7081 tttggaaaag gcgacatact cagccagctt ccagcccatg gagccgtgca gcccggtggt
     7141 agtcacacag atcgccgaat cgcgcaccag gcgcaggtag gttctctttc tcgaaagcgc
     7201 gctgtcctgc aagagctgct tcgggtagcg gcgcgcggcg aattcatcgt gctgaaagcc
     7261 gccgaggaac cggtcgccga ggcgggcgcg catcgccgag atgcattcgg cgcgcatttc
     7321 attcatcgtt tcccgctctt cggcttttct tggcgaaaag cccgcgacac cagctgggtc
     7381 ccaggccttg gcgatgaaca gcacgcgcgc tctcgcgtcg agccgcggcg cggcctgcat
     7441 gtcggaaaca ggcagctgca tgccaattcc gaagtgcagc cccgcgattc gcatcagata
     7501 ggccgcgaat tccttgccgc gcggcgtggc gtacgccgga aatgcgagca ttctgcgcaa
     7561 ttcgtgcagg tcgaagtgat cgccatagac ctcgtagttc aggccaagag gggcgacctt
     7621 gtggcgaaac tgtgaaggaa ccgccgacgg tgagaagctg cgcttgaagt acatatcgca
     7681 gtctctgatc gcgtccgggt cgatttcatt gccgtcgtgt gtatcgaagt agacgcgcag
     7741 gctgtggttg acaatgacca gcaagtgctc acgccgagcg tgccgcagat gactgggggc
     7801 ttccggccgc tcgtaatcaa tatcctgaag ttcctgcgtc aggcggatcg ctccgcgtcg
     7861 tgccagcatg gcgaagcccg tcaggagttg cgacagatgt gcggcgcggc tccacatgac
     7921 caatcggcag tgcgtgacca tggatcgcag agctctggct ggcatggcgg ttcactattc
     7981 ctgcgtcgat cgttacccct caagttgtac tgatatgtca aagcggcggg cagtcggata
     8041 gcgagaagtt cgcccgtaag agagcctgaa cgccgtttgt acggcacgcg ggcctatgcg
     8101 attattcggc ctgatcacct tgcgcaatgc ggcacacttc ccgcaacgca gggtaagtat
     8161 cctcagggca tttggtcggc taacaacgcc ggcactggca aaatctgcat ggcttacctg
     8221 cttacccttc cgatcctcgt ctatgtgatc ggagcgactt tcggaatgcc gctcagtttc
     8281 ggactgggac tgagcctcaa gaacggcgtc gtgtatctag tcggtctcat gctgtgcatg
     8341 cgcatcgcga ttcggggaaa ctacaaattc gagcagccgc tcatctttac ctgtttcgcc
     8401 atcatgatca tctacgcgat cgtcacatgg ttaatggccg gtctggtgat ccactacgat
     8461 gcctaccaga tcgtaccgag cgccatttcg ctcaagggca gcctgatcga caacgccatc
     8521 atcttcacgt tgttcttttt cggtgtgcgt gacgcgaagg attgcattct gctcactaaa
     8581 gtactcgtcg ccgtcgttgc cctttcgaac atcctttgta ttctgtcgat cgagggaatt
     8641 gtgtacatcc acggcgttgc ggcgacgccg ggccaggaag gccgcgtgca gggcgtctac
     8701 ggtgatgcga acgagaccgc cgcattggtg gccggcatgt tgccggcctt tgtggccatg
     8761 gtgctctcgg acggggcggc ctgggcgctg ttgtggattg tctgtgcgat tggctcgatt
     8821 gcgatgctgt tcatgacggt atcgcgcggc gccttcgtcg cgatggggat cgcctatcct
     8881 gtctgcatct atacgttcag acgatacatc gccatacgcc aggtgtttat ctgggcgggt
     8941 gctctggtcg gtttaggtgt agtcgcactg gccgcggcgg ggccacgatt cgtaaccctg
     9001 tttctgactc gattcatcaa tgaatcggat gtgtcctcac tggactacat ttcgtcgggc
     9061 cgcagtgcga tctgggcacg agcgatcggc aagatgatga gttatccgat cacgctcttc
     9121 accggattcg gctggaacgt ttatgacacg atgggattct tctatgttcc gcacaacagc
     9181 tacctgatga tgtggttcga gctgggtctg gtcggcctgc tcagcttcct gttgatcctg
     9241 ttccggattg tgaccacgac gcgtcgcgcg gcggaggtcg cggacccggt ctcgcgtact
     9301 tacctcatcg cttttgtgtt cggcttcgcc gcgtttcttg tggcgatatt cttcgtgaat
     9361 acctacctga catgggcata catgtggatg tacgcggcgc tatcgctgcg gctggcgctg
     9421 tgcgtggcgc aagcgccgca gcgttcccgg ccggccggtg ccggaacggg cagggtgcta
     9481 cgccgctcgc ggcgcaaggg tgtcgcagag cctggctcgg ggcgcgcccg ccccgtggcc
     9541 ggagcgtcct aggtcggtcg ccagcgacgc agaggcttca gcgacgattg acgcgctagg
     9601 ggaacgtcac cagtagaatc gaattctcca ccgcagtggt gacgatcagg ctcttcggaa
     9661 cccccgggat cgaggcaatg ccgtatggat tgttgagcga gcccggcagt gcgccgagta
     9721 tcacgccttc tgatctgggt gcgccgacca ctgtagtcac cgcgcctgcc gttgtgactt
     9781 gtcggatcga catgttgacc gtatcgctga catacaggac gccgttgtcg agtgtgatgt
     9841 cctcgggata gagaaagttt gctgcagagc cgactccgtc cccggggccg ggcgacgcgg
     9901 gattaccagc cagcgtcgtg acgacgccgg cctgtgtgat tgcgcgtatg gtgctgttgc
     9961 cggtatcggc cacgtagagg tttccggcgc catcggacgc aatgccttgt ggtgcgttga
    10021 actgggccgc ggcgcctgcg ccgtcaaggg tacccgtaac gccggcggtt ccagcgatgg
    10081 tcgcgaccgc cccggcggcg gtcacgcgcc ggatagtgtt gttgccggtg tcggtcacat
    10141 agaaattgga ctggccgtcc gtggcaatac cggtcgggcc gttgaatgag gcggcgctgc
    10201 cattgccatc cacggagccg gtggttccta ccgatccagc gacggtgctg acgacgccgt
    10261 cgagagtcac tttgcggatc gtattgttgc ccgagtcggt cacgtaaagc gccgacatgc
    10321 cgtcaaacac gatgcccacc ggcgcgttga aggacgcact gctggcgctg ccatccgccg
    10381 agccggtgac gcccgcggtg cccgcgagcg tggttgccac accaccgagc gtgatcttgc
    10441 ggatcgtatt gttgttgacg tcggcgacgt agacgttgcc ggcgttatca caggtggcca
    10501 gcagcggtcc gttgaatgag gccgccgggc cggtcgcgtc ggtcgagccg ggttgtccct
    10561 gcagccccgc cagcgtcgtg accaatccgc tcgagagttt gcggatggtg ttgtcgccat
    10621 agtcgctcac gtaaatgttg ccaccgccgt ccagtgcgac gccccggggc acgttgaact
    10681 cggcctgtgc ctcggggccg ttcacggtac ccctcaagta gttaccagcg atggtgctga
    10741 ccacaaactg aggggctgcg ctgatcatcc ggatcgtcat gcccccgaga tccgaaacgt
    10801 acaggttgcc ggacgcgtca attgcaatgc cgtagacatt gccaaactgg gcggaagcgc
    10861 ctggcccgtc ggacaggccg aataccccgg cagtgcccgc gatggtcgtg acgacacccg
    10921 tcggagttat ttcgcggatc gtgttgttga gcgtatcggc gacgaagacg ttgccggcgg
    10981 cgtcgaccgt aagtccctcc ggggcgttga accgcgcgcc cgcaccggtg ccatcctgcg
    11041 agccgaccac gcctgcggtg cccgccagtg tcgataccgc cccagctggc gtcatcttgc
    11101 ggatcgtgtt gttgccggtg tcggcgatgt agacattgcc tgccgcatcg gtcgcgatcc
    11161 cgcccggcgc gttgaatgag gcggtgttgc cggttccgtc ggaatttccg acgtttccag
    11221 cggtgccggc gatagtgctc acggtgccat tgggttgaat caggcggatc gtgtcgttgc
    11281 cggtgtccgc cacgtagagg ttgccggagg ggtcgaccgc gacaccgccc gggttggtga
    11341 agtacgcatt ggtgccgatg ccgtcgctcg acagtgcgat ctcggcggtg cctgcaatcg
    11401 tggtgacgac cccgttgggg gagacctgac ggatgatgtc gcgagtggag tctgccacat
    11461 acacattgcc ggccgcgtcg gtagcgatgc ccgccggcgc gtagaagcgg gcgccggcgc
    11521 cggcgccgtc gattgagccg ctgccgccga gctggccggc cagcaatgca acgccgacag
    11581 cggggcctga gaccgtcaat gtagccggct ggctggtcgc actgcctgcg gcattggtga
    11641 ccacgaccgc gaactgcgcc ccgttgtcct gcggaagcgc gggagtgacc gtataggagg
    11701 tgcccgttgc gcccgcgatg ttggctgcgt tgcgcgtcca ttggtaggtc agaggctgcg
    11761 tgccgcttgc tgtgaccgag aagctagccg tgccgccgaa cggcaccgtc acgttctgcg
    11821 gcggcgtcac aatcgtcggg gcgacgatca cgggactgac cgtgagcgtc gcgttgttac
    11881 tggtgacact cccgacacca ttggagaccg tcacggtata gatcgagccc gaatcagaca
    11941 ggctcgccgc gggcgtcacg tagttggcgg tgttggcacc agcgatgacc acaccgttcc
    12001 tgttccactg gaatgccagc ggcgcgctgc cttttgctac cacggaaaag ctggcggtcg
    12061 cgcccggtac gaccgtttgg gaggtcggtt gcgtgctgat cgatggcgcc acggcagtga
    12121 cagtcagggt cgccgaacgg ctcgtggcgc tacccgcggt gttcctgacg atgacagaga
    12181 gcagtgatcc cgtctgagcc agagcagccc cgcgcatcac cagcgtactg cctgttccgc
    12241 ctggcattcc ctgcccgttg atcaaccatt ggtagacgag agcgcccgtg ccgcttgcga
    12301 ccacgctgaa ggagacgggg ctgccgtcgc tgaccgtcgc attctggggc tgctgtacga
    12361 tctgcggcgc ctgcagagcc gtactcggtg cggtcggccc gttggttccc tctagctgag
    12421 caccccacgg agaggagttt gccccgcagc cggtaagcag tcctgcacac accaccagca
    12481 gagtcagtgc agagcagctt gtgggcttga gcatatgggt gcttgatatc cccggtcgtc
    12541 gctttttcgc gacatgatgg gtacagtatc tgggggcgcc gctccgtagg ggtgcaattg
    12601 acagtatgtg cacgcggcta gtgcaaactg agcagtggtt ccgcttacag tatttatagt
    12661 tatattttaa gcgattggtt aagtcgcatc ggtcgccgcc aagcgtgaac ccgtacgatt
    12721 tttctgcgcg ccaaccttta ggatctacga cgtcgcgcga ccggcttcac tgatggattt
    12781 ttgcaacgac tggccgaaat cctgtgaaag ggatttgact aaactcgcaa gagccgcgcg
    12841 tctgtgattt catcagcatc agatagaggc ggtccaatga gccaagtcag tctgatcacc
    12901 ctcaccaagg gcattgcgac cttcgtcccc ggcgtcaaca aactggctac gcgaaggacc
    12961 agcgggacct ccagcgcacg ttattgctat tccgtgtgga tgcgacatct cgccgcagca
    13021 cgcgcggtcg gtgcgccatg gccggtcaaa gcggtcgctg agctcgggcc cggcgattct
    13081 atcggaagcg gcctgtcggc cattctctcg ggggcacaat cgtacatcgg actggacggc
    13141 tttgcgtttg cagcagcgtc atcgaccctt gagatcttcg aggaactggt gtcgctcttc
    13201 aaagcgagag caccaatccc aaatccggat gagttccccg acatctggcc gcgagtggac
    13261 tcctatgatt ttcctcacga cttgctgacg gaatccttgc tgtcggaggc cttggatccg
    13321 tcgcggctgg aggccatccg gaaagcactt cgtggcgggc tcaaatcaga cagcggacag
    13381 gacggcgctg gggtcacggt gcgttacgcc gctccgtggt acgcaaagga ggtcatgcgc
    13441 gagaactctg tggatatgat tttctcgcag gcggtgatgg aacacgtcga taacgtgatt
    13501 gaaacctatc agtcagctcg ccgctggatc aaaacgggcg gcttcatgtc gcagactatc
    13561 gactattcct cgcaccgctt tacgcgtgac tggtacggac actggacatt gtcgagaccc
    13621 atgtggcgca tggtcgcggg acgccgcaaa tacaccatca atcgatggcc ccatagcgcg
    13681 catctgaaag ccatggaaac gaccggtttc cgtatcattg gcgaatcggt gatgcgagac
    13741 gcagcgctcg agcgcgcgca tctggcccct gagtttcgag atctcaccga cgtcgactta
    13801 gctgctcacg ccgccttcat ccagacgtta gctgtctagt cccacagttc agtgcgggct
    13861 cgcgctgatg tttgacggcg ggttcggctt gattgagttc acaggcgtgc ggctcaggaa
    13921 cagcgcgtct ccggtggaag taatggccga gggattgagc gtgaagctcg taccgcagct
    13981 gccattgttc accgacgcat caaccgtatc gttgctgttc tgaaggtgac agtacgacac
    14041 ctcggttgtg acagtcacgg cggcgccgcc tgtcgtttgc cgcggcagta ccagaatgat
    14101 tccgttctgg aagtcgcgac gatagacgcc atttttccat gcacaggtgt tgccgttggt
    14161 ccaggtgttg ctaaccggta cgccgcagga attggagacg ttgtttggat tcgccggcag
    14221 cccaagccat ttgacgttct tgagagtgcc accttgctct tcgtcaaacc atggccatgt
    14281 tgttgtattg ctgccatcga tagagtcagc gctggggtag taagaggtca ggccgaatgt
    14341 gcaatacccg atgtccatgg tgcacatgcc gaaggcgtag cggaagtatt gatagtcggc
    14401 cgccgtgctg ttcgcgaccg gaccggtcac catgacatag ggcgcgccgc cggcaacctg
    14461 actcttgtat tcattcatcg cataggtgaa accgccccag gtttcgaccg cgttggacag
    14521 ccccgttccc cagatgaatt gctgcatggc gccgtcgaat gcgccgacgc cgctggcgta
    14581 attgacgccg aggctgtcag tccagcccag agcctggttg acgaagcata gtttgttccc
    14641 gctcgcactg tgaagtgccg gacatagctg caactgcgcg ttctgaatgg cggtgtccag
    14701 agcaagcggt gtattcgcca gcgtgcccgg caaagtgcca ttgcgtagcc aatccccgga
    14761 aaatttggcc gtggggagcg tgttgtcgaa gtagaagaag tcgacattcg gggcagcatt
    14821 gttcgcattt gccccgttga ccggaccacc tttgcccagt ccgtacgtgt tccacacgta
    14881 gctcgcaaac cactggtaag gcgtttgccc gctgtagctg ggagcgtagg tgctgaaatt
    14941 cacgagtgcc atgtggccat cgctgtcgtt cacggcggtc gcgttattgc agctggttcc
    15001 aagcgggcac agataccaac cggcagtctg gacctgccgg tcctcgttgg cagaagcgga
    15061 aagattggcg ccgcttggaa cttcaagaat ggagtagcgc ccgaccaggt gcacaggtga
    15121 aaccatggtg ccattgctgg cagcgtggat accgttgacc acgtttccga tgttgactcc
    15181 ctggttttcc cagttacagc cgagcatcac gatgtcgtag cgcgcagcca gcgtgtagtt
    15241 ggcggacgaa atattgcact gtgcgcccga gatggcctgc accatcgtgc gcgggtagcc
    15301 aattgtgctg ttcacgtccg gggagccgaa cgaagcggac gaaagcgaaa gccctgccaa
    15361 agcaacccat agcgttacgc gcatcttgaa atcctccagt tttccccacg ctgcccggcg
    15421 catgcgctgc cagcaacgtc caccatcaac aatcccacgt tagtaaaggg cactacctgc
    15481 cgtcgagcaa tgtgcctcat cagtatcgcc agaactgagc cagtcgtcgc agtttgtgca
    15541 atgtcggctt gctttctggt cgatcatggc ggctgcgtta catggcgggc ggatctcctg
    15601 tctgaaagaa gcttgccgcc agcaacgccg gccaattgct gtgacagcat tcaacaatag
    15661 gcctgatgag cgcgcaacca gtcgcggtgc accgtcatgt cggcaacaac cgacagggtg
    15721 cttggaaacc gcttctctag ttgcttcgct tatttgcgcg aggcgttaag cggcattgcc
    15781 gactcgagcg cgccctgaat gccgagcgtc gtgaacgcct cgacgtatgc gttcacggcg
    15841 gtgtggggat tgtagtcccg gtccatgaaa tcaatggaac gagtgctcgc tctttgccac
    15901 tcgtcgctat cgtcagtgaa tgcgtcgact gccgtccgta actcttcaag gttcgccacc
    15961 gcgcgtccga tcgaattttg tgcaatcaaa ttatccgggt cgagaaacgt caccacaggt
    16021 acgccatgtc cccaggcctg caagtaggta ttggggaagc cttcgatttc cgaggtgttg
    16081 acaaatacgc gcgtacgctc gaagtaagag ttcacctcgt ggtacgaaac tgagccatgg
    16141 aagtggatgt tcgggcaagc tgcggctttt tcccttactg aattgaaata atccaccaga
    16201 ccgtcccacg caccgccgat catgtgaaaa cgaagatgcg gcaattctcg cgcaagctcg
    16261 atgaacatct ccggtcgttt gagcggccgc aggttcgcaa cccacagcgc atcgatatcg
    16321 cgctgttcgc gcgtgaggcg ccgaccgatc atgccgccaa tcgcggggac gaccttgctg
    16381 tcgcggcggt agttcctgcg cagcatctgc tgctgcaggg ctgactgcgt caataccaga
    16441 tccgcgcggc gcaggccctg ggcatataat ttcttgtcgc gccacagctt caccaatagc
    16501 gcgttgggat cgcagtccga gtcgctcatc gtaccgaata caaccttgcg gccgaacatg
    16561 cgggtaaaaa gactcacctg ccagatcaac acgccggcgc aaccgacata gtagatgtct
    16621 gcgtccgcgc ggcgcagggc cgcccagacg ctgctccagc gcgggtgaaa gaaacggagg
    16681 ccgggaagac ccgcattgag ccgataggca cggtaggtag tcacgccgcc ccagaccgca
    16741 ccatcgggtt ggccgtgatc ggccacgacc atcgatacct tgaagtcacg tgcggccagc
    16801 gccttggcca agacgctttg ctgcacctcg gcgccgccgt ttccgagatg gccgtactcc
    16861 ggcgccagtg ccggtaagtt tccaatgccc acgaaacaga tgctgggctt gttcaggacc
    16921 gcagtgctcc gttacggaag ttcaattcgc gatcgcgctc ttatctggcc gccgtgcgta
    16981 atcagaatgg gaggctttgg tgaactcaat gacccgatcg acctggtagt ccttgatgtt
    17041 cttggcgtgc gtgaagatga tcagctctcc cagcagtccc agcgcaaaca attgcaagcc
    17101 caacacaacc aatagcgagg agagcaatag cgccggtcgg tctgccagac cgacattgaa
    17161 gatcaatcga tcgaccacca ggtaggcgag cagagctccg ccgatgacaa aggtcgatac
    17221 acccagcatg ccaaagaagc gcagcggctt cttggtgaag cgcaccagaa agaagacggt
    17281 gaaaatatcg agcacgcggt gagcgtattc gcgcgcccgg tagtggccgg tgaatcgatc
    17341 cagcgccgac tgcgcaacgt tgatttcgcg caccctgaag ccctgccgat ctgccagcag
    17401 cgccaggaaa cggtgctgat caccgtacag cgtgatttct tcgagcaccc ggcggcgcat
    17461 gacgcgtgcg ccgcagccca ggtcatgaaa tcgctggccc gtgacactgg caatcagacc
    17521 atgaaatgcc gatcgccgca gaccttcgaa tgagccgccg gcccgcggcc accggcgtgc
    17581 gatcacgaga tcagcctcat ccaggcccgc caccagcttg gtgatttcca tgccctgtat
    17641 ctggtgatac gcgggtaaat tgatgatcaa ctggcccgca gcgcgcgcga agcccgccat
    17701 gagcgcagtg gcttcaccga aggggcgcgt caatgacacg atcgaaatgt cgttgtgttc
    17761 ggcggtcagg tcttgcagtg cggtcgcgaa gtcagtctgc ggaccatcca gcacaaagat
    17821 gatctcgtag cgctggccta ccgcggccag gccggcggag tactcagcaa gcagggattg
    17881 cggatcagcg tgccgatccc cgaccgggat aataacggac aactgaatct cggcgtcgct
    17941 ttgcgtcatg gcaacctcag gcgccgctcg caggtggctt aagaacacgc tcagtaagcc
    18001 gggcgaaatc gtattcgcgc atgtcgccgg tattgtacac aagctcgcca aaggcccccg
    18061 cgctcaccag tatgaacgcg gacatcagaa agatgagtcc ggtaccagcc acgggcagcg
    18121 gcatgccgcg atgctcgctc accatgacgt acccgctgta ggccaaagcg aacaagccga
    18181 ttgccaccag cggcagtgac agcagtgtga accagatcag cggccgcgcg gtgaaagatg
    18241 ccacggtctt gatgaccagc agatccagca gcactttata gattcgagac agtccatact
    18301 tcgacttacc gaactgccgc gcgtg
//
LOCUS       DATMRM010000306        10788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4532257, whole genome shotgun sequence.
ACCESSION   DATMRM010000306 DATMRM010000000
VERSION     DATMRM010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10788
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4532257"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="VNZ06_09075"
     CDS             complement(<1..1024)
                     /locus_tag="VNZ06_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWW20943.1"
                     /translation="MIFEQIATGGCRSYLIGCQRSCAAVLIDPELTQIDRYLGLATHH
                     SVRIRYLIDTHTHADHFSATRELARRLEVPIVMHQASPAPFVDMRLGDGESIVLGDLR
                     IHVMHTPGHTRDSMCLRVDNHLFTGDTLLIGATGRTDLPTGDPEALHDSLFNRLLRMD
                     AALEVHPAHEYKNRSHTTLAQEIEHNPRLQKRERAEFVEMMRNLNLSMPTHLTEALRT
                     NLSGGTTITQLLADAAQLVSFMSLTELKSRVDTHDSELVILDVRERDAYAAGHVPGAR
                     LLPRGQLELRVNDELPDPTLRILTICEFGKLSTLAAATLRQIGFGRAIALDGGMKAWR
                     EAGYPTV"
     gene            1089..1232
                     /locus_tag="VNZ06_09080"
     CDS             1089..1232
                     /locus_tag="VNZ06_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20944.1"
                     /translation="MKRTMLFRYSHISGLLSLIIGLAAEAAAAGHLEDPGAQDLGRRT
                     ARL"
     gene            complement(1287..2870)
                     /locus_tag="VNZ06_09085"
     CDS             complement(1287..2870)
                     /locus_tag="VNZ06_09085"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HWW20945.1"
                     /translation="MAAAAVLAACGGGGGSGGLTDPATPPPTTSFAGRVTFKGAPLAD
                     ATVVAFDANSNATYATTTTDANGNYSFSGFNTSCTDNCTQTYVIWAFKSGYAFYPVMA
                     SNASGSRSTYQWDAASSTWYLASGAAVTRAGLNGQFTDGSSGAGIIFTTYNFSSVTQG
                     PKGPTDSVSGANFMAYDGSNPLVSLAASGQSVSYAAGDDAALHVGVSWPGTRFADNQD
                     GTVTDHLTGLVWLKDGGCLQPNLWSQAIGEVNELASGACGLSDGSVAGQWRMPNMWEL
                     ESLIDESAANPAVSAGSPFVNINGIYWTSTSYYGGIGGSPIAWAIRMADGRYINDGAT
                     NVKQSGSLGVWAVKGAGGGAVKLQATGLYVPFVAGDDGNAQAGVPLTFPRMRDNADGT
                     VTDTVTGLIWLKQANCINDTWAGALADVSALASGQCGLTDGSTAGSWRMPNRKEMESI
                     ADRAQNNQSDFFDTNWTSGNSTINSMNAVFSNFIQLQYYWTSTTNASDVTEAWTVFSC
                     DYGVYDISKGSTGYTLAVR"
     gene            3304..6027
                     /locus_tag="VNZ06_09090"
     CDS             3304..6027
                     /locus_tag="VNZ06_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWW20946.1"
                     /translation="MRRLQALQLACRNLLSRSAAFVARPGIVARLIVAFLAVGVLVFA
                     ANFLVVQAVLIERSTTVYQTIAPGEGKSRTSSHPAVPVLPVQRVDTAILRAALIRYRA
                     AVRGRIESNTDRAEGEQLAAAQELQHALSILSPAAAEIGRAADVERIRSLLSGYEADA
                     AAAITAADERRADIRRYDSLADELYALTKQSVDRSWKVFGLVIARHSLLDYSAALDDL
                     RRIATPEDLRGVEEPESRAQHLLIANESAFRRSLGAAWYTTQRKQLDQLVSLREKSAS
                     LTSELAGLNEHNLELGENLASSVPDVLRGSLPRAAVAVAAAPAQDPPPTIAPVVPAVS
                     APVTTQSVTYEDDHDRQRLIALISGGVLLLLIIIGVGTVLSIERPVRRLLAATARIAR
                     GETAVRVERGGIRELDTLAVAFNQMADELQQARSVALEYQQSLELKVAERTSELKNFA
                     ENDYLTALPNRRRFFAMLNDALLKAGHARRLLGVLFLDVDNFKYINDSLGHAFGDRVL
                     LSLANRLQHTVQPVGIAARFGGDEFTVLVDGVHSVDEIRAVGEQIVKAFQAPLSIDGR
                     DLIVGVSVGASIYPEHEREAETLLRAADAALFRAKKLGRSQLAVFTSDLLETAASNFT
                     TEQGLRRAIERGEFELVFQPEVDSSSFETIVVEALIRWRTHDGRLMSPADFLSVAEEA
                     GLIVEISDWVLGRAVQTAARWYHGSWPEVRVAVNISPRQLLDRGFASRLQRLLQEAQL
                     PARCIEIELTESVLQTGPTTLDALRQLQAQGISIALDDFGTGYSSLSSLQQLPLSRVK
                     LDRSLIADMDANARSRSMVRAIIGMCRQLGLEITAEGVERFEQFALLAGQKIYVQGFL
                     LSPPVAEEELAGARERIASRAEEFLITSRTGTHPVVHELSASRRTRVLNDR"
     gene            6151..7113
                     /locus_tag="VNZ06_09095"
     CDS             6151..7113
                     /locus_tag="VNZ06_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWW20947.1"
                     /translation="MKTGITALAVILAALSPAQAARRPVSELTQLATLRLGKTADWVA
                     IANNAVWVGATGPNAVVQIDPRNNSLGATVLLPGEPCAGLAVGFGSLWVPLCATPNEL
                     VRVDLRTHAITQFTHVGPADREGGVTVSTDSVWLILDRRGTLARIDPQSGRIRQKIRV
                     PPGSYNPLYGDGQIFVTHADGAEVTAVDANSGAILANIPTGPKPHFLTTSPGAVWSLN
                     QGDGTLTRIDSHTHDVRSTTALHTPGHGGDIKFGEGKIWSTLESVPLSIVNATSGELE
                     CQWSGPGGDSLDIGHGSLWLTNYEAGTISRYDLTTVLKACYGTL"
     gene            complement(7238..8971)
                     /locus_tag="VNZ06_09100"
     CDS             complement(7238..8971)
                     /locus_tag="VNZ06_09100"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HWW20948.1"
                     /translation="MSLRAAGAILALALAAGAAVAGGGTVHDLVFRNGVIYDGSGGPP
                     FRGDLAVDGDRISYVGPHASGGARTEIDVKGQAIAPGFINMLAHPEESLFADGRALSD
                     LTQGVTLEVIGEDSMGPLNEAMRKLMVARQSDIKYAVTWNTLGEYLETLERRGIAPNI
                     ASFVGAPAVRTYVLGEGDVQPTPQQLRQMQGLVHQAMEQGALGVTTALIYSPATYART
                     AELIALAQESARCGGIYTAHMRSEGDRIEAALQETIDIAKASGAPAEIYHLKVSGRDN
                     WGKLDGVIAQVERARASGVRLSANMYTYTAGATGLDAAMPSWVQDGGLEAWIARLKDP
                     SIRAQVIAEMRTLHPATWENLYAAAGAEGTLLLAFKNPVLKPLTGKTLAAVARERGVS
                     PEDVAIDLVIEDGTRVGVAYFLMSEDNVRREVALPWVSFGSDEAGDAPEGVFLLSAAH
                     PRAYGNFARVFAQYVRKDHVLSVQEAVRKLSALPADNLSLPQHGRLLTGKVADVVVFD
                     PDTIQDHATFDRPHQLSTGVSYVLVNGKLALREGKATGAATGRVVRGRAWKGASSGGC
                     RASAQAWTWSH"
     gene            complement(8977..10248)
                     /locus_tag="VNZ06_09105"
     CDS             complement(8977..10248)
                     /locus_tag="VNZ06_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWW20949.1"
                     /translation="MMLSVWILTAAVSGAFSHPTPAPAADIPVEVYRQRRERVLNSLE
                     GCLAVVSAQGGVSGVTEDFRQDGDFFWLTGINEPDAHLILAPRSRYTKTTLYLKSRDP
                     EAERWTGPRDSVSPALLSRYGVDRVRRGMPDAGLMDAAQEYDCIAIIVPASAGARDRN
                     DAELSLRVASRYGLRVVYRRGLMAGLREAHGPEEIARMQQAIAITRKGHDVAARATIA
                     GVSERDVQTQIEYAFYAAGATGLSYPSIVGSGPNGAVLHWDQNSRLLSDGDLVVIDAA
                     GEYGRYAADVTRTYPVTGHFTEEQAAVYRAVYQAQEDIFAAIRPGVTMADLQHAAEES
                     LRRSGHLADFIHGFGHFVGLDVHDAGAYEKPLPAGAIITVEPGVYLPQRNFGVRIEDM
                     VLITDKGYRLLTADIPRKLEDVEAWIAAARR"
     gene            complement(10295..>10788)
                     /locus_tag="VNZ06_09110"
     CDS             complement(10295..>10788)
                     /locus_tag="VNZ06_09110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWW20950.1"
                     /translation="LSDELLPDLQPKAKEYPPGTHPFGKRTVHAAPWMLFNLSAYTRM
                     LMSDYLANGGKIEIVEFHSASDLVAVRQRTMVNATGYGARALFGDESVVPVRGQLARM
                     IPQPDIHYGLFYKGTSFIPRRDGMVFQMVGENDYYGYGDDSAQPDRAEAALAVNTIAG
                     LFA"
BASE COUNT         1993 a         3537 c         3307 g         1951 t
ORIGIN      
        1 tcaccgtcgg gtagccggcc tcccgccagg ctttcatgcc accatcgagc gcaatcgcgc
       61 gcccgaatcc gatttgccgc aacgtcgcgg ctgcgagcgt ggacagcttg ccgaactcgc
      121 agatcgtcag gatacgaagc gtcggatccg gcagttcatc attgacgcgc agctcgagct
      181 gcccgcgcgg cagcaggcgc gcacccggta catggccggc tgcataagcg tcacgctcgc
      241 gcacatccag gatgacgagt tcggaatcgt gcgtgtcaac ccgggacttg agttcggtca
      301 gtgacatgaa cgacacgagt tgcgccgcat cggccagcag ttgcgtgatg gtcgtgccgc
      361 ccgacagatt ggtgcgcagc gcctcggtca gatgtgtcgg catcgacaga ttcaggttgc
      421 gcatcatttc gacgaactca gcgcgctcgc gcttctgcag acgcggattg tgctcgattt
      481 cctgggccag cgtggtgtgg ctacggttct tgtattcgtg cgccggatgc acttcgagcg
      541 ccgcatccat ccgcaacaga cgattgaaca ggctgtcatg cagtgcttcg gggtccccgg
      601 tgggcaagtc cgtcctgccg gttgctccga tcagcaatgt atcgcccgtg aacagatgat
      661 tgtctactcg cagacacatc gaatcgcgtg tgtgcccagg tgtgtgcatg acatggatgc
      721 gcagatcgcc cagcacgatc gattctccgt ctcccaggcg catgtcgacg aacggcgccg
      781 ggcttgcctg atgcatgaca atcggcacct ccagccgacg agcaagttcg cgggtagccg
      841 aaaagtgatc ggcgtgggta tgcgtgtcga tgaggtatct gatccgaaca ctgtggtgcg
      901 tcgcgagacc caaatagcgg tcaatctgag tcagctccgg atcaatcaga accgccgcgc
      961 agctgcgctg acaaccaatg agataagacc tgcagccacc ggttgcaatc tgttcgaata
     1021 tcatgaccac ccccgtccgt cgtgaaacgc actatgcacc agtctcacct ctctggagca
     1081 gcgtagactt gaaacgaacc atgttgttcc gatattccca catcagcggg ctgctgagcc
     1141 tgatcatcgg gctcgcggcc gaagcggccg ccgccggaca tctggaagat ccgggtgcgc
     1201 aagacctggg tcgcaggaca gctcgtctat agcgagacta ccgagagcga tcgacagcgc
     1261 ccctgatgaa tccgaccgcc cggaaactat ctcaccgcga gcgtatagcc ggtgctgccc
     1321 ttcgagatgt cgtagacgcc atagtcgcag ctgaacaccg tccaagcttc ggtgacatcc
     1381 gacgcattgg tggtggaagt ccaatagtac tgaagctgga tgaaattgct gaacacggca
     1441 ttcattgaat tgatggtcga attgccgctg gtccagttgg tatcgaagaa atcggactga
     1501 ttgttctgcg cgcgatcggc gatgctctcc atttcctttc gattcggcat gcgccagcta
     1561 ccggcggtcg atccgtccgt cagcccgcac tgaccgctcg cgagcgcact gacgtccgcc
     1621 agcgccccgg cccaggtatc gttaatgcaa ttggcctgtt tcagccagat cagtccggtc
     1681 acggtatcgg tcaccgtgcc atccgcattg tcgcgcatgc gcgggaaggt caatgggacg
     1741 ccggcctgcg cgttgccgtc atctcctgcc acaaacggca catagagacc ggtggcctgc
     1801 agtttcacgg caccgccgcc cgcgcccttc accgcccaga cacccagcga tccgctctgc
     1861 ttgacgttgg tggcaccatc attgatgtaa cgcccgtccg ccatccggat ggcccatgcg
     1921 atcggtgatc cgccgatgcc gccgtagtag ctggtcgagg tccagtagat accgttgatg
     1981 ttgacgaatg ggctgccggc actcacggcc ggattcgccg cactctcatc gatcaggctc
     2041 tcgagttccc acatgttcgg catgcgccac tggccggcga cggacccgtc gctcaaacca
     2101 cacgcgccac tcgcaagctc gttgacctcg ccgatcgcct gcgaccacag gttgggctgc
     2161 aggcagccac catccttcag ccaaaccagt cctgtgagat ggtcagtgac cgtgccgtcc
     2221 tggttgtcgg cgaaacgcgt gcccggccac gacacgccga cgtgaagcgc agcgtcgtct
     2281 ccggccgcgt agctgacgct ctggccgctc gcggcaaggc tcacgagtgg gtttgatccg
     2341 tcataggcca tgaaattggc gccgctgacc gaatcggtcg gacccttcgg tccctgggtc
     2401 accgaagaga agttgtaggt cgtgaagatg atgccggcgc cgctcgagcc gtcagtgaac
     2461 tgaccgttca gaccggcccg tgtcactgcg gctccacttg ccagatacca ggttgaggaa
     2521 gccgcgtccc attgataggt actgcgacta ccggacgcgt tgctcgccat caccggatag
     2581 aacgcgtagc cacttttgaa cgcccagatc acataggtct gggtgcaatt gtcggtacag
     2641 gaggtgttga aaccactgaa gctgtagttg ccattggcat cggtcgtcgt cgtggcatac
     2701 gtggcgttgc tattcgcatc gaatgcaacg accgtcgcgt ccgccagcgg cgcacccttg
     2761 aaagtcactc ggccggcgaa tgacgtggtc ggcggcgggg tcgcgggatc ggtcagtcca
     2821 ccgctaccac caccacctcc gcaggctgca agcacggcgg cggcggccac ggcaaaccat
     2881 ccgtttttcc gatgacatat cctaccgaga cgagtgttat gtcctgcgcg gtgcatgcac
     2941 gatccctgtc aggcgatgaa tatccagttg gttatcggcc agcccaggcg ctggtgtagg
     3001 cgcgcagagc ggcgcggtac gtgaccggat tcagggattc gaagcgcggg cgcgcccgcg
     3061 ggacataacg cgcccgccgc gcttgctggt tgccagcgcg ctgatagcct gcaagcaaca
     3121 ctaacgacga catgccgcta cgaaacggca ttgagatagg ttctagttgc ccgctctgcg
     3181 tgccatgaaa ctggcgacat gctcgttgat gaacgtgcgc tcgtcacaga ttgcagtgcg
     3241 cacaatcatc ggccgcgccc ggaccgctat attcgaaccc cgaagacagt acatcgcggc
     3301 cggatgagac gactccaagc cttacaactc gcctgccgca atctgctgtc gcgatcagcc
     3361 gcgttcgtgg cccgccccgg tattgtcgct cgcctgatcg tggcgttcct cgctgtgggt
     3421 gtactggtgt tcgctgccaa tttcctggtc gtgcaggccg tactgatcga aagatcgacg
     3481 accgtctacc agacgatcgc gccgggggag ggcaagtcgc ggaccagctc ccatcccgcg
     3541 gtcccggtgc tccccgtgca gcgtgtcgac accgccatcc tgcgcgccgc cctcatccgc
     3601 tatcgcgcag cggtgcgtgg ccgcatcgag agcaacaccg atcgcgcgga aggcgagcag
     3661 ctggccgcgg cgcaggagct gcaacacgcg ctcagcatcc tgtcgccagc agcggccgaa
     3721 atcggccggg ccgccgacgt cgagcgcatc cgctcgctgc tcagcggcta tgaagccgac
     3781 gccgcggcgg cgatcaccgc cgccgacgag cggcgcgccg acatccgacg atacgattct
     3841 ctggccgacg agctgtatgc gctcactaaa cagtccgtcg accggtcctg gaaggtcttt
     3901 ggcctggtca tcgcgcggca ttcgctgctg gactacagtg ctgcgctcga cgatttgcgg
     3961 cgcatcgcga cacccgagga tctgcgcggt gtcgaagagc ccgaaagccg ggcacagcac
     4021 ctgctgatcg ccaacgaaag tgccttccga cgctcgctcg gcgccgcctg gtacaccacg
     4081 cagcgcaaac agctcgatca gctcgtgagc ctgcgcgaga agagcgcgag cctgacttca
     4141 gagctggccg gtctcaacga gcacaatctc gagttgggag agaatctggc cagctcggta
     4201 cccgacgtgc tgcggggctc cctgccgcgg gccgcggtgg cggtggccgc agcacccgcg
     4261 caggatccgc caccaacgat tgcgcccgtc gttcccgcgg tcagcgctcc ggtcaccacg
     4321 caatcggtga cctatgagga tgatcacgac cggcagagat tgatcgcgct catcagtggc
     4381 ggtgtattgc tgctgctgat catcatcggc gtcggcacgg ttctgagcat cgagcgcccg
     4441 gtacgccggc tgctcgcggc cacggcacgc atcgcccgtg gtgagacggc ggtccgcgtc
     4501 gaacgcggtg gcatccgcga actcgacacg ctggcggtag cgttcaatca gatggccgat
     4561 gagctgcaac aggcgcgctc ggtcgcactg gagtaccagc agagcctgga actgaaggtc
     4621 gcggagcgta ccagcgagtt gaagaacttc gccgagaacg actacctcac ggccttgccg
     4681 aaccgcagac gcttttttgc catgctgaac gatgcgctgc tgaaagcggg gcacgctcgc
     4741 cggctcctgg gtgtgctgtt cctggatgtg gacaacttca agtacatcaa tgacagcctg
     4801 gggcacgcgt tcggcgaccg ggtactgctc agtctcgcca atcgactgca gcacaccgtc
     4861 cagcctgtgg gcattgctgc gcgattcggt ggtgatgaat tcacggtgct cgtggacggg
     4921 gtgcactcgg tcgatgagat ccgcgcggtc ggcgagcaga tcgtcaaggc ctttcaggcg
     4981 ccgctctcga tcgatggccg cgatctcatc gtcggcgtca gcgtcggcgc cagcatctat
     5041 cctgaacacg agcgggaagc cgagaccctg ctgcgagcgg ccgacgccgc gctgtttcgc
     5101 gccaagaagc tcggtcgcag tcagctcgcg gttttcacct cggacctgct cgagaccgcg
     5161 gccagcaact tcaccaccga acaggggctg cggcgcgcga tcgagcgcgg tgaattcgaa
     5221 ctggtgtttc agcctgaagt ggattcatcc tcattcgaaa ccatcgtggt ggaagcactc
     5281 atccgctggc gtacccacga tggacggttg atgtctccgg cagacttcct gtcggtcgcg
     5341 gaggaagcgg gcctgatcgt cgaaatcagt gactgggtcc tcgggcgcgc ggtacagacc
     5401 gcggcgcgct ggtatcacgg gtcgtggcct gaggtgcgcg tcgccgtgaa catctccccg
     5461 cgtcagctgc tcgaccgcgg ctttgcttcg cgcctgcaaa gactgctgca ggaagcgcag
     5521 ctacccgcca ggtgtatcga gatcgaactc accgagtcgg tgctgcaaac cggtcccacc
     5581 acgctggatg cgttgcgcca gctgcaggcg cagggcattt cgatagcgct tgatgacttc
     5641 ggtaccggct attcttcgct gtcatcgctg cagcagttgc cgctgtcgag agtcaagctg
     5701 gatcgctctc tcattgccga catggacgcc aacgcccgtt cgcgttcgat ggtacgggca
     5761 atcatcggca tgtgccgcca gcttggactg gagatcacgg ccgagggcgt cgagcgattc
     5821 gagcagttcg cgctgctcgc cggccaaaag atctacgttc aaggctttct gctgtcgcca
     5881 cccgtcgcgg aagaggaact ggccggcgcg cgtgagcgca tcgcctcgcg cgccgaggag
     5941 ttcctgatca ccagcagaac cggcactcac cccgtggtgc atgagttgtc cgccagccgt
     6001 cgcactcgcg tgctgaacga tcgctgaagt aggggcggaa ccacagggct tcagcgctca
     6061 tcggcatcga tcaatcgaac aaagtgccgc ttacccgcac gtgacgagtg agtgaatgtg
     6121 cagcggcggc gaagtgataa agtggtcccc atgaagaccg gtatcactgc cctggccgtg
     6181 attctcgccg cgctgtcccc ggcccaagcc gcacgccggc cggtcagcga actgacccag
     6241 ctggcgacgc ttcgcctcgg aaagacggcc gattgggtcg cgatcgccaa caacgcggtc
     6301 tgggtcggcg cgactggacc gaacgccgtg gtgcagatcg atccgcgcaa caactcattg
     6361 ggcgcaaccg tactgctgcc cggtgagccc tgtgcaggat tggcggtggg attcggcagc
     6421 ctgtgggtgc cactgtgcgc caccccgaat gaactggtgc gcgtggacct gcgcacccat
     6481 gcgatcacgc aattcaccca tgtcgggcca gcggaccgag aaggcggggt cacggtcagc
     6541 accgacagtg tctggctgat cctcgacagg cggggaacgc tggctcgtat cgatccgcag
     6601 tctggcagga tccgacagaa gattcgggtt ccgccgggct cctacaatcc gctctacggc
     6661 gacggtcaga tctttgttac ccacgcggac ggcgcggaag tcactgcggt cgacgccaac
     6721 agtggagcca tcctggcgaa catcccgacc gggcccaaac cgcacttcct cacgaccagc
     6781 cccggcgctg tgtggagctt gaatcagggc gatggcacgc tcacgcgcat cgacagtcac
     6841 acgcacgacg tcagatcgac gaccgcgctg catacgccag gccacggcgg cgacattaaa
     6901 ttcggcgaag gcaagatctg gtccaccctc gaaagcgtgc cgctgtcgat cgtgaatgcc
     6961 acgagcggtg aactggagtg ccagtggagc ggcccaggcg gcgattcact cgacatcggc
     7021 cacggctcac tgtggctgac gaactacgaa gccggaacca tctcgcgcta cgacctcacc
     7081 accgtactga aagcctgtta cggcacactg taagccgcta agccttcaga accgcactcg
     7141 aatctggccg ccgatgatcc gcggattatt cctggcgagc gtagctttcg gatggcgcac
     7201 tggaaaccgt agcgctcaac aacgacccgc ggccttgcta gtgcgaccag gtccaggcct
     7261 gcgccgaggc acggcagcca ccgctcgacg cacccttcca ggctctgccg cgcaccacgc
     7321 ggccggttgc cgctcccgtc gctttgcctt cccgcagcgc cagcttgccg ttgaccagca
     7381 catagctgac gccggtgctc agctgatgtg gcctatcgaa ggtcgcatga tcctggatgg
     7441 tatccggatc gaacacgaca acgtcggcga ccttgcccgt gagtagtcgt ccgtgctgcg
     7501 gcagcgacag attgtcggca ggcaatgcgc tgagcttgcg caccgcttcc tgcacgctca
     7561 gcacatgatc cttgcgcacg tactgggcga atacgcgagc gaaattaccg taggcgcgcg
     7621 gatgcgctgc ggacagcaga aacacgcctt ccggcgcatc accggcctcg tccgatccga
     7681 aactcaccca gggcagcgcc acctcccggc gaacgttgtc ctcactcatc aggaaatagg
     7741 cgacgccgac gcgcgttcca tcttcgatga ccagatcgat cgccacatcc tccggcgaga
     7801 caccgcgctc gcgggccacc gccgccaggg tcttgccggt cagcggcttg agtacgggat
     7861 tcttgaacgc cagcagtagc gtgccttcgg cgcccgccgc ggcatacaga ttctcccagg
     7921 tcgccggatg cagcgtgcgc atctcggcaa tgacctgggc gcggatggac ggatccttca
     7981 gccgtgcaat ccaggcctcc aggcccccgt cctggaccca ggagggcatc gcggcgtcca
     8041 gtcctgtggc ccccgcggtg taggtgtaca tattggcgct gagacgcacg cccgacgcgc
     8101 gcgcacgctc tacctgcgca atgaccccgt cgagcttgcc ccaattgtcg cgcccggaaa
     8161 ccttgaggtg atagatctcg gcaggtgcac cgctcgcctt cgcgatatcg atggtctcct
     8221 gcagcgcggc ctcgatccgg tcgccctcgc tgcgcatatg cgccgtgtag atgccgccgc
     8281 atcgggccga ttcctgcgcc agcgcgatga gttccgccgt cctggcatag gttgcaggcg
     8341 agtagatcag agccgtggtc acaccgagtg cgccttgctc catcgcctga tgcaccaggc
     8401 cctgcatctg cctcaactgt tgcggtgtcg gctgtacatc gccttcgccc agtacatagg
     8461 ttctgaccgc gggcgcgccg acgaaggacg caatgtttgg agcgatcccg cgacgctcga
     8521 gcgtctccag gtattcaccc aaggtgttcc aggtcaccgc atacttgatg tccgactggc
     8581 gcgcaaccat caactttcgc atggcttcat tcaacggacc catcgagtcc tcgccgatca
     8641 cctcgagcgt gacgccctgc gtgagatcgc tgagcgcgcg gccatcggca aacagcgatt
     8701 cttccgggtg cgcgagcatg ttgatgaagc cgggcgcgat cgcctgacct ttgacatcga
     8761 tctcggtgcg cgcgccgccg ctggcatgcg ggccgacgta actgatgcga tcgccatcga
     8821 ccgcgaggtc accgcgaaac ggcggcccac ccgagccgtc atagatgaca ccgttgcgaa
     8881 acaccagatc gtggaccgtg ccgcccccgg caaccgcggc acctgcggcg agtgcaagag
     8941 cgaggatcgc gcccgcggcc ctcaagctca tggacctcat cgtcgcgccg ctgcaatcca
     9001 cgcttcgacg tcctccagct tgcgcggaat gtccgcggtc agcagtctgt agcccttgtc
     9061 ggtgatcaac accatgtcct cgatgcgtac gccgaaattg cgctgcggca ggtacacacc
     9121 cggctctacc gtgatgatgg caccggcagg caaaggcttt tcgtaggcgc cggcatcgtg
     9181 cacgtcgagg ccgacaaaat ggccgaagcc gtggatgaag tcggccagat gcccggatcg
     9241 ccgcagcgac tcttcagccg cgtgctgcag gtccgccatc gtcacgcccg gcctgatcgc
     9301 cgcgaaaata tcctcctgcg cctgatagac ggcgcgatac accgcggcct gctcctcggt
     9361 gaagtgaccc gtgaccggat aggtgcgggt aacgtcggca gcatagcgac cgtattctcc
     9421 agccgcatcg atcacgacca gatcgccgtc gctgagcagg cgactgttct gatcccagtg
     9481 cagcactgcg ccgttgggac cactgccgac gatggaggga taagacaacc cggtcgcacc
     9541 ggcggcgtag aacgcatatt caatctgcgt ctgtacgtcg cgttccgaaa ccccggcgat
     9601 cgtggcgcgc gcggccacat cgtggccctt gcgcgtgatg gcaatcgcct gctgcattcg
     9661 ggcaatctcc tccgggccgt gcgcttcgcg caggcctgcc atcaggccgc gtcggtaaac
     9721 cacccgcagg ccgtagcggg aagcgacccg cagcgaaagc tccgcgtcat tgcgatcgcg
     9781 agcgccagca ctggccggca cgatgatggc aatacagtcg tattcctgtg cggcgtccat
     9841 caaccccgca tcaggcatgc cccgccgtac cctgtccacg ccgtaccggc tcagcagcgc
     9901 cggtgacacc gaatcacgcg gcccggtcca gcgctcggcc tccggatcgc gcgacttgag
     9961 gtacagcgtc gttttcgtgt agcgcgacct gggcgccaga atcaagtggg catcgggctc
    10021 gttgatgccg gtcagccaga aaaaatctcc gtcctgacga aaatcctcgg tgacaccgga
    10081 cacaccgccc tgtgcggaaa ccaccgccag gcagccttcg agtgaattga gcacgcgctc
    10141 gcggcgctgg cgatagacct cgaccggaat atccgccgct ggagcggggg tgggatgaga
    10201 gaacgcgccg ctcacggcgg cagtgagaat ccacacactg agcatcatga ccccctgaga
    10261 ttctcctggt cgtccgctac gccctggtcg tgcgctacgc gaatagtccc gcgatggtgt
    10321 tgacggccag cgccgcctcg gcgcgatcgg gctgggcgct gtcatcgccg tagccgtaat
    10381 agtcgttctc gccaaccatc tggaatacca tgccgtcgcg ccgcgggatg aagctcgtcc
    10441 ccttgtagaa cagcccataa tgaatatccg gctgcgggat cattcgcgcc agctgcccgc
    10501 gaaccggaac cacgctctcg tcgccgaaca acgcccgtgc gccgtagccg gtcgcattca
    10561 ccatcgtcct ctgacgcacc gcgaccagat cggaggcgct gtgaaactcg acgatttcga
    10621 tcttgccgcc gttggccaga tagtcggaca tcagcatgcg tgtgtaggca ctcagattga
    10681 acaacatcca cggtgcggca tgcacggtgc gcttgccgaa gggatgcgta ccgggaggat
    10741 attctttggc cttgggctgc agatcgggca gcagctcatc ggacagct
//
LOCUS       DATMRM010000307         4546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_804792, whole genome shotgun sequence.
ACCESSION   DATMRM010000307 DATMRM010000000
VERSION     DATMRM010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804792"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1641)
                     /locus_tag="VNZ06_09115"
     CDS             complement(196..1641)
                     /locus_tag="VNZ06_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWW20951.1"
                     /translation="MSKRRRNWRRRTTLALATSLLGSALHAQTPSAPDRPWPIPESAT
                     ERAAALETGPGDWVKKPYDLPALIDLAQRANPETRAAWEAARAAAAAIGLVESSYLPQ
                     LSMEALGGFERTPLPIPKNLDPKGYFVSESREIIPSLALKWLLFDFGRRDANLEAARA
                     DSFVANAGFTAVHQKIVLEVSQTYFALGASRGRLRAARKALTTAQTVETATQAKRDRG
                     LATIVALAQAQRQTAQARYSVAADEGAERTAMANLIAALGIPAATPLEVSDASDTPPP
                     ASPNQSIAAAINAAMAHRPDIIAALGKVDSAEASLKGAQRAYRPTIGLSAQVFQNVGA
                     LRSDGGPTSTVNQAGGSILFSFSIPLFDGGERASRISIARAKVRESQADLSQTRDSVA
                     QQVVRAYDELLSSLAEYDAAETVRRAADTSYDAALHSYQQGVGTYTDLAAEENAVADA
                     ERQVEDARAGAHTAAAGLAFAMGLNAVAQAP"
     gene            complement(1613..3307)
                     /locus_tag="VNZ06_09120"
     CDS             complement(1613..3307)
                     /locus_tag="VNZ06_09120"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HWW20952.1"
                     /translation="MERPASFLAFLGQELAPEEGRGLAVVRIAAACTITVAIAMVFRI
                     PLAAYMAYMVFLTSKDDSAATVTTGVGAVLAITLAVILTLGLSQIALGDPAIRLPAMA
                     LTTFLAMFSARTFALGPVSFLAGFLIVTVQSVVDVEGPEAFTRTTLWLWVVVVVPVVT
                     TVLINLLFGPLASALAERGVREVLTDLDSALTSGQIAGRLVQWRAILVPLIEKSRNPA
                     AIQRLLDALVILEAYPDPIPLRDRQRLSQLLRGCLSALERRSPVAEETPETASSPEAL
                     AATHAFTELQRELSRSDAHQASPAGHKPRQLFVPDMFSNPAHWQFALKTTLAIMIVYS
                     VYTMLDWPGLRTSIITCFFVALGSVGETVQKLLLRLSGAIIGGLLAGLSIVFLLPHFT
                     DIGQLSVLTAVVALFAGWVATSSERLSYAGLQIAFAFFLGLLQTDSPATDLTVLRDRV
                     VGILLGNIVMTVVFSTLWPESAITRLRAALADAQRYTATLLSSRVEQSANRQRTIEAL
                     TRARHWEELSRFELDLLPDRISQLARLPDVNEIERRAAAAFVATETEHVQTSPQLA"
     gene            complement(3312..4355)
                     /locus_tag="VNZ06_09125"
     CDS             complement(3312..4355)
                     /locus_tag="VNZ06_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWW20953.1"
                     /translation="MSPTDANSGVTQRGKLIALGSIAAALVLGTLVVFAIDRRPRTHD
                     ARLFAFSAAMASEINGRIVRVCIANDQYVSKGSPLILIDPVPYDMQLREARAQVAALK
                     AQIGLTGRQVESQDSGAQAAESQVQKAREQLKYARDTRERLEPLVAPGYVTQQQLDEA
                     RTNESSALAALSALTKSATQAHQAVGDTLSLAAQLEGAEAAEALAARNLELTTLRAPF
                     DGVVVGLQIAEGTFALSGRPLFSLIKSDAWYAIADFRETELPRIAVGDRATVWTMADS
                     GRAISGRVESLGSGVQSSDQDGPGLPQVGRDLNWVVVAQRFPVWVRLNSPPAEAMHVG
                     MTASVKVHHGDSH"
     gene            complement(4352..>4546)
                     /locus_tag="VNZ06_09130"
     CDS             complement(4352..>4546)
                     /locus_tag="VNZ06_09130"
                     /inference="COORDINATES: protein motif:HMM:NF028400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HWW20954.1"
                     /translation="PSRSILGSYFPSWMVCALIGLVAALVARVAFKATGLLGELPAPL
                     VVLLSIGCATTFALWLVWLA"
BASE COUNT          896 a         1390 c         1465 g          795 t
ORIGIN      
        1 ggttcaggga gagaaacaag gggcttgccg cagcgtattc ataggtgggg agcgggtaat
       61 ctcgcagcga accagaggac acgcccaccg agtgcgtcga gatgctctac gaaggtcaga
      121 cgcggagggg gaacctcaag attacgcgcc gccacgcctg atattggtgt ccgcatatat
      181 ggtggattgc aacggtcagg gagcctgagc gaccgcgttg aggcccatcg caaaggcgag
      241 ccccgccgcc gcggtgtgcg cgccggcgcg cgcatcttcc acttgtctct cggcgtcggc
      301 tacggcgttt tcttctgccg caaggtccgt ataagttccg acaccctgct ggtaagagtg
      361 aagcgcggca tcgtaggacg tatcggccgc ccggcgcacc gtttccgccg cgtcatattc
      421 cgcgaggctg ctcaacagct catcgtaagc gcggactacc tgctgcgcga cgctatcacg
      481 cgtctgcgac aaatcggctt gtgactcgcg caccttcgca cgtgcgatcg agatacggct
      541 ggcgcgctcg ccgccatcga aaagcgggat gctgaacgaa aacaggatgc ttccgccggc
      601 ctggttgacg gtcgaggtcg gtccgccgtc cgagcggagc gcgccgacgt tctggaatac
      661 ctgggcactg agcccgatcg tcgggcggta ggcacgctgc gcgcctttca gggacgcttc
      721 cgccgaatcc actttcccca gcgcagcaat gatatcgggt cgatgtgcca tcgctgcgtt
      781 aatggctgcc gctatactct gattgggcga cgcgggcgga ggagtgtccg aagcgtcact
      841 cacttccaat ggcgtggccg ccggaatgcc gagcgcggcg atcaggttgg ccatggccgt
      901 gcgctctgcg ccctcgtcgg cagcgactga atagcgagcc tgtgcggtct gtcgttgtgc
      961 ctgagccaga gcgacgatcg tggcaagacc acgatcgcgc ttcgcctgcg tcgctgtctc
     1021 gacagtctgc gcagttgtca gcgccttgcg agccgcacgc aagcgtccgc gcgatgcgcc
     1081 gagtgcgaag taggtctggg agacttcgag cacgattttt tggtggacgg cggtgaagcc
     1141 tgcgttcgcc acgaacgagt cggcgcgcgc cgcttcgaga ttcgcatccc gacgaccgaa
     1201 gtcgaacagg agccacttga gcgcgagcga aggaatgatc tcgcgtgact ccgatacgaa
     1261 ataacccttc gggtcgaggt tcttggggat cggcaacggc gtgcgctcaa aaccgcccag
     1321 cgcttccatc gaaagctgcg gcagatagga actctctacc aggccaatgg cggcggcggc
     1381 ggcgcgtgcc gcttcccagg ccgcgcgcgt ttcagggttg gcgcgttggg cgagatcgat
     1441 gagtgccggc agatcgtagg gtttcttaac ccaatcaccg ggaccagtct ctagagccgc
     1501 ggcgcgctca gtggcggact ctggaatggg ccagggtcgg tcgggagccg aaggtgtctg
     1561 cgcatgcaac gcacttccca gaagggacgt cgcgagcgcc agtgttgttc gcctacgcca
     1621 attgcggcga cgtttggaca tgctcggtct cggtcgcgac aaaagcggcg gcagcccgac
     1681 gttcgatctc gttcacgtcg ggcaaccgcg ctagctgcga aatacgatca ggtagcaaat
     1741 cgagctcaaa gcggctcagc tcttcccagt gtcgcgcgcg cgtgagagct tcaatcgtgc
     1801 gctgccggtt ggctgactgc tccaccctgg agctcaggag cgtcgcggtg tagcgctgcg
     1861 cgtctgctaa cgctgctcgg agtctcgtga ttgccgactc gggccatagg gtgctgaaca
     1921 ccactgtcat tacgatattc cccaagagga tacccaccac tctgtctcgc aaaaccgtca
     1981 ggtcggtggc tggcgagtcc gtctgcagca accctaagaa gaaagcgaag gcgatttgca
     2041 ggccggcata cgacagccgt tcgctgctcg tcgcgaccca acccgcgaac agcgcgacga
     2101 ccgcagtgag cacggataac tggccgatgt cggtgaagtg aggaaggagg aaaacgatcg
     2161 agagtccggc aagcagtcca ccgatgatgg cgcccgagag tcgcagcagc agcttttgca
     2221 ctgtctcgcc gacgctaccg agcgcaacga agaagcacgt tatgatggaa gtgcgaaggc
     2281 caggccaatc gagcatcgtg tacacgctat acacaatcat gatcgcgaga gtcgtcttca
     2341 gtgcgaactg ccaatgagcg ggattgctga acatatcggg cacgaacagt tgacgtggct
     2401 tgtgcccggc gggagaagcc tggtgcgcat ctgagcgaga gagctcgcgt tgtagctcag
     2461 tgaaggcgtg agtggcagca agtgcctccg gcgacgacgc cgtttcggga gtctcctcgg
     2521 ccacagggct tcgtcgctcc agtgcgctga ggcaacctcg aagcagttgg gacaggcgtt
     2581 gtcggtcgcg cagtggaatg gggtccggat acgcctcgag aataaccagc gcatccagca
     2641 accgctgaat cgcagctgga ttccgggact tctcgatgag cggcaccagg attgcgcgcc
     2701 actgcacaag tcgcccggcg atttggccgc tagtgagcgc ggagtcgaga tctgtgagga
     2761 cctctctaac gccgcgctcg gccagggccg atgccaaggg cccgaagagc agattgatca
     2821 gtaccgtcgt caccacggga acgactacga ccacccatag ccacagggtc gttcgcgtga
     2881 atgcctccgg accctccaca tcgacgaccg actgcaccgt cacgatcagg aagccggcga
     2941 ggaaggaaac aggcccgagc gcaaaggtgc gggcgctgaa catagccagg aaggtcgtca
     3001 aggccattgc gggcaggcgg attgccggat cacccaaggc gatctgtgag agtcccagtg
     3061 tgagaattac tgcaagcgtg atggcgagca ctgcgccaac gcccgtcgtt acagtcgcgg
     3121 cactgtcatc cttgctcgtc aggaagacca tgtaggccat gtacgcggcg agcggaatgc
     3181 gaaacaccat cgcaatcgca accgtaatcg tgcaggccgc ggctatacgc acgacggcga
     3241 gcccgcggcc ttcttccggc gcgagttctt gcccaaggaa cgcgagaaag gacgcggggc
     3301 gttccacgcg tctagtgaga gtcgccatga tgaactttga ccgatgccgt catgccgaca
     3361 tgcatcgctt cggccggtgg tgagttcaga cggacccata cggggaaacg ctgcgccaca
     3421 accacccagt tcagatcgcg gcccacctgg ggcagaccgg ggccgtcctg atccgatgac
     3481 tgcacgcccg aacccaaact ctcgacgcgc ccactgatag ctcgcccgct atccgccatc
     3541 gtccacaccg tcgcgcgatc gccgacagcg attcgcggca gctcggtttc gcggaagtcc
     3601 gcaatcgcat accaggcatc ggacttgatg agactgaata gcggacgacc ggaaagtgcg
     3661 aatgtgcctt ccgcgatctg cagcccgacg actacaccgt cgaatggtgc gcgcagggtc
     3721 gtgagctcga gattgcgcgc cgcaagtgct tctgccgctt cggccccttc caattgagcg
     3781 gcgaggctca gcgtgtcgcc gacagcctgg tgagcttgag tggcgctctt ggtcagtgcg
     3841 ctgagcgcag cgagcgcaga ggactcgttg gtacgcgctt cgtccagctg ctgctgggtg
     3901 acataaccgg gagcgacgag tggctccagg cgctcgcgcg tgtcccgggc gtacttcaat
     3961 tgttcgcgtg ctttttgaac ctggctctcc gccgcttgtg caccggaatc ttgcgattcc
     4021 acctgcctgc ccgtgagtcc tatctgcgct ttgagcgctg cgacctgcgc gcgggcttcc
     4081 cgaagctgca tgtcataagg aaccggatcg atcagaatga gcgggctgcc cttggaaacg
     4141 tactggtcgt tggcaatgca gacacgaacg atgcgtccgt tgatttcgga ggccatcgcc
     4201 gcgctgaacg caaacagacg agcgtcgtgg gtgcgcggcc gccggtcaat tgcgaacacc
     4261 actagcgttc cgaggaccag tgcggcggcg atcgagccga gcgcgatgag ctttcctcgc
     4321 tgcgtgactc cgctattcgc gtcagtgggg ctcatgcgag ccacacgagc cacagggcaa
     4381 acgtcgtcgc gcagccgatc gagagcagca caaccagcgg cgcaggcagc tcaccgagca
     4441 gcccggtagc cttgaacgcg acacgcgcga ccagtgcagc cacgagccca ataagggcac
     4501 acaccatcca cgacgggaaa tacgacccga gaatgctgcg cgaggg
//
LOCUS       DATMRM010000308        11435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3565145, whole genome shotgun sequence.
ACCESSION   DATMRM010000308 DATMRM010000000
VERSION     DATMRM010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3565145"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /gene="accA"
                     /locus_tag="VNZ06_09135"
     CDS             complement(<1..724)
                     /gene="accA"
                     /locus_tag="VNZ06_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HWW20955.1"
                     /translation="MAVSFLDFEQPIAELEAKIEELGHVSSDTEVNIEEEMTKLRAKS
                     RALTRSIFSSLTPWQITQLARHPQRPYTLDYVASMLTDFQELHGDRMYADDLAIVGGL
                     ARLEGAPVMVIGHQKGRDTKERVRRNYGMPKPEGYRKALRLMKMAERFGLPLITLIDT
                     PGAYPGVGSEERGQSEAIARNLFEMSTLRVPVVTVVIGEGGSGGALAIGVCDRLLMLE
                     FSTYSVISPEGCASILWKSADKK"
     gene            complement(807..2150)
                     /locus_tag="VNZ06_09140"
     CDS             complement(807..2150)
                     /locus_tag="VNZ06_09140"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWW20956.1"
                     /translation="MKLCKSISPVILIGLVSGCSLARSISVASAGTSHFLCSAVFVSG
                     LDADSTYRKLMAPKAGMNVINWTVHYQVDRERREVTTTIGGGFRSRALYRDGLGCVLQ
                     HDDDPLNIPVPLAASRADELPLVRPADARLQAALDQAFEDTPHQAPLNTQAVVLMHRG
                     QIIAERYASDGGIETPYAGWSMSKAVTNALIGILVRQGRLELDTQAPLNRSVSVNQLL
                     RQTSGIDLLQTESGFDPMSQALLLERDRVQYTRGRGFSATPGHEWRYTNGNYLVLSRM
                     LRDAVGGDAPHVLRFAHENLLDPIGMHRATIEFDATGTPYGETFVYATARDWARLGQL
                     YLDDGVVSGRRILPQGWVAYSTTQTLDTGYGASFWLNLTHTRIPHTGLLNVAWGLPGV
                     PPDAYFARGSFGQYVVIVPSEQLVVVRLGVSQAPLEHIQSVGRLVTEVIAALHAP"
     gene            complement(2228..3289)
                     /locus_tag="VNZ06_09145"
     CDS             complement(2228..3289)
                     /locus_tag="VNZ06_09145"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWW20957.1"
                     /translation="MGLQSADLMIRGGVLALLLLIAVLLLREHARATVARLGAAFAIS
                     TAAYVICSFWSHNDPVSDWQAPLYFVGGANCVLLWLLASALFDEEFHLRSWHAALWVA
                     MGLLTVTCLRLFGSSALHPFLQAGQLLATLSFAALALLRTVATWRADLLEVRRRLRLF
                     VVAAAALHLMISSLVRFLPMSARAVPLWSIANAAILLLIVGTITWLLLRTDIEALLPV
                     EEAAPPPAPAADEVYTSPSLIAEVERSMTRDQLYRDGALTIGALAAKLGLLEYKVRRI
                     INQGLGYRNFNAFLNHYRVEHAKAMFAERRQADTSVLSVAMDCGFQSLGPFNRAFKAQ
                     TGMTPSEFRRIRPVHSKIG"
     gene            complement(3298..6819)
                     /gene="dnaE"
                     /locus_tag="VNZ06_09150"
     CDS             complement(3298..6819)
                     /gene="dnaE"
                     /locus_tag="VNZ06_09150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWW20958.1"
                     /translation="MTATFVHLRLHTEYSLIDSVVRVPGLMQAVAAAGMPAVALTDQG
                     NLFAMVKFYREALSNGVQPIIGVDLMLRGLDEKQGPSRLTLLCQDLVGYQNLARLVSR
                     AFLEGQSQTAVPLVDRTWLTRETTAGLIALSGSHEGDVGRALLHDRMAQAEVALEFWC
                     SLFEDRYYLELQRIGRPEEQRYLPAALKLAVRYDLPVVATNDVRFLRAEDFESHEARV
                     CIHDGALLADAARPRRYTPQQYLRTAAEMQRLFEDVPEALANTVEIARRCSLQLTLGQ
                     ARLPDYPVPCGIDVNEYLRGQSALGLETRLSALAASEEAAVHYRARLASELDVICPMG
                     FAGYFLIVADFIRWAREHGVPVGPGRGSGAGSLVAYALRITDIDPLRYDLLFERFLNP
                     ERVSMPDFDVDFCMEGRDRVIEYVAEKYGHDKVSQIITYGTMAAKAVVRDVGRVLGHP
                     YGFVDRIAKLIPFELGITLEDALAKEPELKRLYAEDEDVRGLLDLARSLEGLTRNAGK
                     HAGGVVIAPSVLTDFTPLYCEHGGTSVITQFDKDDVEAAGLVKFDFLGLRTLTVLDWA
                     VATINASRTASDQPPLELGALPMDDVATYALLKACTTTAVFQLESRGMKDLIRRLQPD
                     CFEDIIALVALFRPGPLQSGMVEDFINRKHGKNQAAIDYLHPDLKPVLTPTYGVILYQ
                     EQVMQIAQVLAGYTLGGADLLRRAMGKKKPEEMAKQRSIFVDGARARGVSDRVAAHIF
                     DLMEKFAGYGFNKSHSAAYALLSYQTAYLKTHYPAAFMAAVLSADMDHTDKVVTLIDE
                     CAKIGLTVQPPDVNSSRYAFAVADARTIRYGLGAIKGVGRSAVEALVEERERGGRYRT
                     LVDLCRRVDLTRLNRRVFEALIRSGSLDGLAENRATLMARLDEALLGGEQNARRHEAG
                     QGDIFAGNAAVAASESLNLKPLAEWTASQRLAGERETLGLFLTGHPIAPFEADLPYFA
                     SGRIAEFASERASGPIDPARSYIDARNITLAGLIVEIRRRGPRVSFMLDDRSGRMEAT
                     MFEEVYQRHRDLIVKDALVQVEGALRFDEFSDAWRVAVKQVQKLEAVRERLARRLVLS
                     GNAAQFNGQTLLRLEGLLRDSLGGQCTILLCYRGTEAAGTLALSESWRIRPSSTLLEK
                     LEHLLGASSVRLVYGQEISSNSAALAR"
     gene            complement(6832..7464)
                     /locus_tag="VNZ06_09155"
     CDS             complement(6832..7464)
                     /locus_tag="VNZ06_09155"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWW20959.1"
                     /translation="MRELIAGVDEAGRGPLAGPVVAAAVILDPAQRIRGLRDSKQLSA
                     ERREVLAERIRERALAWAVAQSDHDEIDAINILQATLLAMRRALLGLSARPTQIIIDG
                     NCAPQLHDCFAECVIRTVVGGDASVACVSAASIIAKTYRDAWMVRQDERYPGYGLGKH
                     FGYCTRSHVKALVALGPSAIHRRSFNPLKSWLSGELRFGTLEMFPETAQE"
     gene            complement(7570..8757)
                     /gene="lpxB"
                     /locus_tag="VNZ06_09160"
     CDS             complement(7570..8757)
                     /gene="lpxB"
                     /locus_tag="VNZ06_09160"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HWW20960.1"
                     /translation="MPAASALKIAIVAGETSGDTLGGALIRALRERVATLEVRGVTGP
                     AMREAGCETLADAHELAVMGFIDPLLQLPRLLRLRSMLIERITAWKPDVFIGIDSPAF
                     NLGLAGRFKQQGIATVQYVSPQIWAWRQGRVRSIARRCDLILCLLPFEPAFYRDHAVR
                     AQFVGHPLADQIPLTSDRAAARASLGLPADPAQPVLALLPGSRLSEVRHLGSTFIAAA
                     LELHRRIPQLRLVAPMANAAARAEFSRALAATPGAGELPLKLLDGQAREALTAADAAL
                     IASGTATLQALLCRCPMVVAYRFGSLTAFLARTLRLVRLKYFSLPNLLAGEQVAPEFL
                     QEAVTSANLSAAVEQTLRDVPRRHYLEQKFLQVHRALQAGGAERAADEILRMLRERAP
                     SSR"
     gene            complement(8769..9539)
                     /gene="lpxA"
                     /locus_tag="VNZ06_09165"
     CDS             complement(8769..9539)
                     /gene="lpxA"
                     /locus_tag="VNZ06_09165"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HWW20961.1"
                     /translation="MISAQAIVASGAKIAPDAQIGPFSIIGADVVIGARTVVAPHVVI
                     NGHTTIGQDNRIFQFSSLGEAPQDLKHRGEPTRLEIGDRNSIREYCTISLGTVHDRAV
                     TCIGSDNLLMAYTHVAHDCVIGNNVVMSNLAMIGGHVHLGDWAILSGYAAVHQYVRIG
                     AHAFIAHHAGCTKDVPPYVMAVGNPAVPHSINIEGLKRRGFTAEQIRNLKNAYRILYR
                     SDLPLDTAVKQLNELAASQPELKVLLSFIENSERSLIR"
     gene            complement(9536..10024)
                     /gene="fabZ"
                     /locus_tag="VNZ06_09170"
     CDS             complement(9536..10024)
                     /gene="fabZ"
                     /locus_tag="VNZ06_09170"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HWW20962.1"
                     /translation="MNDATNLDINSVMLRLPHRYPFLLVDRVLDYVAGQSIRALKNVT
                     YNEPYFTGHFPNRPLMPGVMIIEALAQVAGLLAFLTANAFPDDNTRFYFVGIDKARFR
                     KPVAPGDQLILTANFERNFKGIWRLATRAEVGDEEVANALIMIAPDLKSSGGAAAVPE
                     LP"
     gene            complement(10021..11028)
                     /gene="lpxD"
                     /locus_tag="VNZ06_09175"
     CDS             complement(10021..11028)
                     /gene="lpxD"
                     /locus_tag="VNZ06_09175"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HWW20963.1"
                     /translation="MSIALGEIAVRFGCELRGDPDTRVDHIAPLESAGPGAISYLANP
                     RLTPLLASTRASAVVLDPRSADGSAVAALLDRNPHATFARIAALLYPSPVPHPGIHPS
                     AIVDCSASVDPSAEIGPYSVIGAGARIGARCLVGATCLVGRNARIGADGRLVSRVTIE
                     HDVMIGERVLIHPGVVIGADGFGLARDSDGWIKVPQVGSVRIGDDVEIGANTTIDRGA
                     MDDTVISSGVKLDNQIQIGHNVFVGEHTAIAGCTGVAGSTRIGARCMIGGHVGFAGHI
                     QICDDVVITGKSAVPHSIKQPGIYSGVVGLEPVRTWHRIVARMKLGVRREQRLRKDDT
                     E"
     gene            complement(11059..>11435)
                     /locus_tag="VNZ06_09180"
     CDS             complement(11059..>11435)
                     /locus_tag="VNZ06_09180"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HWW20964.1"
                     /translation="ALKTKEESLQRDSATMTPEQRNQAEKELREGNRQLSLKVNDLQD
                     EFNSRQNEELSKLQKTLVDEVQAYAQTQKFDLVLADGVIWAGPSLDITPQILSALQAR
                     GPRAPSTGSATPPKSTSPSNGK"
BASE COUNT         2128 a         3790 c         3516 g         2001 t
ORIGIN      
        1 ccttcttgtc ggcactcttc cacagaatcg acgcgcaacc ttcgggcgag atgaccgaat
       61 aagtgctgaa ctcgagcatc agcagccgat cgcacacacc gatcgccagt gctccgcccg
      121 acccgccctc accgatcaca acggtgacga ccggtacgcg cagtgtggac atctcaaaca
      181 ggttgcgcgc gatcgcttcg ctctgtccgc gctcctccga acccactccg ggataggccc
      241 cgggcgtatc gatcagtgtg atcagcggca agccgaagcg ctcggccatt ttcatgagcc
      301 tgagggcttt gcgatagccc tcaggcttcg gcatgccgta gttgcggcgc acgcgctcct
      361 tggtatcgcg ccctttttga tggccgatga ccatgaccgg cgctccctcc aggcgcgcca
      421 gcccgccgac gatggccaga tcgtcggcgt acattcgatc gccatgcagc tcctggaagt
      481 ccgtcagcat cgaggcgacg taatcgagcg tgtaggggcg ctgcggatgc cgcgcgagct
      541 gagtgatctg ccagggtgta agactggaga aaatcgaccg tgtcagcgcg cgactcttcg
      601 cgcgcagctt ggtcatctcc tcctcaatgt tgacttcggt atccgaactc acatggccga
      661 gctcttcgat cttggcttca agttcggcaa tcggctgctc aaaatccagg aagctgacgg
      721 ccatctaggg tcgactcttt tccctctaag cgggcctgaa gggtacgggt aggcgccctg
      781 ccgcgcaagg cgcccccgat cggcgctcaa ggggcatgca gtgccgcgat cacctccgtc
      841 accagccggc caacgctctg gatatgctcc agtggcgcct gcgatacgcc caaacgcacg
      901 accaccagct gctccgaggg cacaattaca acatactgac cgaagctgcc gcgggcaaaa
      961 taggcatccg gtggtacgcc cggcagaccc caggcaacgt tgagcaagcc ggtgtgcggg
     1021 atgcgagtgt gagtcaggtt gagccagaag ctcgcgccat atccggtatc cagcgtctgc
     1081 gtggtcgaat aggcaaccca gccctgcggc agaatgcgcc gcccggagac gaccccatcg
     1141 tccagataga gctgtccgag ccgcgcccag tcacgggcgg tcgcgtagac gaaggtctcc
     1201 ccgtagggtg taccggtagc gtcgaactcg atcgtcgcgc gatgcatgcc gattggatcg
     1261 agcaggttct catgcgcgaa gcgcagcaca tgcggtgcgt cgcctcccac ggcatcgcgc
     1321 agcatgcgcg acagcaccag atagttgccg ttggtatatc gccattcgtg gccgggggtc
     1381 gcgctgaaac cgcgcccgcg ggtgtactgc acgcggtcgc gttcgagcag gagtgcctgc
     1441 gacatcggat cgaagcccga ctcggtctgc agcaggtcga tgccgctggt ctgacgcagc
     1501 aactgattga cgctcaccga gcgattcagc ggcgcctgtg tgtccagctc gagccgtccc
     1561 tgcctgacca ggatgccgat gagtgcgttg gtgacggctt tgctcatcga ccagcccgca
     1621 tagggcgtct cgatcccacc gtccgaggca taccgttcgg cgatgatctg tccgcggtgc
     1681 atgagcacga cagcttgcgt attgaggggt gcctggtgtg gggtgtcttc gaacgcctga
     1741 tcgagcgcgg cctgcagccg cgcatcggcc ggacgcacca ggggcagctc gtccgctcgg
     1801 ctggctgcga gggggacagg gatgttcagc ggatcatcat cgtgctgcag gacacagccc
     1861 aagccatcgc ggtagagcgc gcgcgacctg aatccgccac cgatggtcgt ggtcacctca
     1921 cggcgctcgc gatcaacctg gtagtgcacg gtccaattga tgacattcat tcctgccttg
     1981 ggcgccatca gcttgcggta ggtggaatcg gcgtccaggc ccgagacaaa gaccgcggag
     2041 cacaggaaat gactggtccc ggccgaggcc acagagattg accgagcgag cgagcaaccg
     2101 ctgaccaacc cgatcagaat gaccggactt attgacttgc agagtttcat tttgccgcct
     2161 ccgtttcagt ggtgacggcg atttcaatgg caagcgcctg tccgaggctc gccggagtcg
     2221 aaatgcgtta gccgattttg gaatgcaccg gccggatgcg ccgaaattcg ctgggtgtca
     2281 tcccggtctg ggccttgaac gcccgattga aggggccgag agactgaaaa ccgcaatcca
     2341 tcgccacgct cagaaccgag gtatcggcct gacgccgctc cgcgaacatt gccttcgcgt
     2401 gctcgacgcg gtagtggttg aggaaggcgt tgaaattgcg atagccaagt ccctgattga
     2461 tgatccgccg gactttgtac tccagcagac ccagcttcgc cgccagagca ccgatcgtca
     2521 gagcgccatc gcggtagagc tgatcgcggg tcatcgagcg ctcgacttcc gcgatcaggc
     2581 tggggctcgt atagacctca tcggccgcag gcgccggtgg cggtgctgcc tcctcgaccg
     2641 gcagcaacgc ctcgatgtca gtgcgcagca gtagccaggt gatcgtgccg acaatcagca
     2701 gcagaatcgc tgcgttggcg atgctccaga ggggcaccgc acgcgccgac atcggcagaa
     2761 agcgcaccag gctgctgatc atcagatgca gggcggcggc ggcaaccacg aacagtcgca
     2821 accgacgccg cacctcgagc aggtccgcgc gccaggtagc cacggtgcgc aacaacgcga
     2881 gcgcggcgaa gctcagcgtg gcgagcagct gtcccgcctg cagaaacgga tgcagcgccg
     2941 atgacccgaa gagtctcaga caggtcaccg tgagtaggcc catggccacc cacagcgctg
     3001 cgtgccacga tcgcagatga aactcttcgt cgaacaacgc gctcgccagc agccacagta
     3061 gtacgcagtt ggcaccgccg acgaaataga gcggcgcctg ccagtcgctg accggatcgt
     3121 tgtgtgacca gaacgagcag atgacgtagg cagcggtgct gatggcaaag gctgccccga
     3181 gacgcgccac cgtcgcgcgc gcgtgctcgc gcagcagcag cactgcgatc agcagcagca
     3241 gtgccaggac gccgccccgt atcatcagat ctgccgactg aagccccata ggcctaccta
     3301 gcgcgccagc gccgccgagt tggacgagat ctcctggccg tataccagac gcacggaact
     3361 tgccccgagc agatgttcca gtttctccag cagcgtgctg ctcggccgga tcctccatga
     3421 ctcgctcagc gccagcgtgc cagcggcttc ggttccgcgg taacacaaca ggatcgtgca
     3481 ctgcccgccc agcgaatcgc gcagcagtcc ctccagacgc aacaatgtct gtccgttgaa
     3541 ctgcgctgca ttgcctgaca gcaccaggcg ccgtgccagt cgttcgcgca cggcttccag
     3601 cttctgcacc tgcttgacgg cgaccctcca ggcgtcgctg aactcatcga agcgcagcgc
     3661 gccttctacc tgcaccaggg catccttcac gatcagatca cgatgccgct ggtagacctc
     3721 ctcgaacatg gtcgcttcca tgcgaccgct gcggtcatcg agcatgaaac tcacccgtgg
     3781 accgcgccgg cggatctcga cgatcagacc cgcgagcgtt atgttgcgcg cgtcgatata
     3841 ggagcgcgcc ggatcgatcg gtccgctcgc ccgctcgctc gcaaactcgg cgatgcgccc
     3901 ggatgcgaaa tacggcagat ccgcctcgaa cggcgcgatg ggatgtccgg tcaggaacaa
     3961 gcctagcgtc tcgcgctcac cggccaggcg ctgtgacgcc gtccattccg ccagcggttt
     4021 caggttgagg ctttcgctcg ctgccaccgc cgcattgcca gcgaagatat cgccctgtcc
     4081 ggcctcgtgc cggcgcgcgt tctgctcgcc cccgagcagc gcctcatcca gccgcgccat
     4141 caaggtggcg cggttctcag ccaatccgtc gaggctgccc gatcggatca gcgcttcgaa
     4201 cacgcggcgg ttgagccgcg tcagatccac ccgccggcac agatcgacca gcgtgcgata
     4261 gcggccgccg cgttcgcgtt cctcaaccag agcctcgacc gccgagcgac cgacaccctt
     4321 gatcgcgccc aggccataac gaatcgtacg tgcgtcggcc acagcgaatg cgtagcgcga
     4381 tgaattcacg tccggaggct gcacggtcag tccgatcttc gcgcattcat cgatcagcgt
     4441 gaccactttg tcggtgtggt ccatgtcggc cgacaacacc gctgccatga aggcggccgg
     4501 atagtgcgtc ttcagatacg cggtctggta ggacaggagc gcgtacgccg ccgaatgcga
     4561 tttattgaac ccgtagccgg cgaatttctc catcaggtcg aagatgtgcg cggcaacgcg
     4621 atcactcacg ccacgggcgc gggcaccgtc gacaaatatc gaccgctgct tcgccatctc
     4681 ctccggcttc ttcttaccca tcgcccggcg aagcaggtcg gcgccaccga gcgtatagcc
     4741 ggccagcacc tgcgcgatct gcatcacctg ctcctgatac aggatcacgc cataagtggg
     4801 cgtcagtacc ggcttcagat ccgggtgcag atagtcgatc gcggcctggt tcttaccgtg
     4861 cttgcggttg atgaagtcct cgaccatgcc cgactgcagg ggacccggac gaaacagcgc
     4921 caccagcgcg atgatgtctt cgaagcaatc cggctgcagg cgacggatca gatccttcat
     4981 gccacgcgac tcgagctgga atacggccgt cgtcgtgcag gctttcagca acgcataggt
     5041 tgccacatcg tccatcggca aggccccgag ttcgagcggc ggctggtcgc tcgcggtgcg
     5101 actggcgttg atcgttgcca cggcccagtc cagcaccgtc agcgtgcgca gacccaggaa
     5161 atcaaatttc accaggcccg ccgcctcgac atcgtccttg tcgaactgcg taatgacgct
     5221 ggtaccgccg tgctcgcagt acagcggcgt gaaatcggtc agcaccgagg gcgcaatcac
     5281 cacgccgccg gcgtgcttgc cggcgttgcg cgtcaggccc tcgagcgagc gcgccagatc
     5341 gagcaggccg cgcacatcct catcttccgc atacagacgc ttgagctctg gttccttggc
     5401 cagcgcatcc tcaagtgtga tcccaagctc gaacggaatc agtttggcga tgcggtcaac
     5461 aaaaccgtag ggatggccca gcacccggcc cacatcgcgc accacggctt tggccgccat
     5521 ggtgccgtag gtgatgatct gggagacctt gtcgtgcccg tatttttcgg ccacgtactc
     5581 gatgacccga tcgcgccctt ccatgcagaa gtccacgtcg aagtccggca tcgacacgcg
     5641 ctcgggattc aggaagcgct cgaacagcag gtcgtaccgc aacgggtcga tgtccgtgat
     5701 gcgcaacgca taggccacga gcgagccggc gcctgagccg cgccccgggc cgaccggcac
     5761 gccatgctcg cgtgcccagc ggatgaagtc ggcgacgatc agaaaatatc ccgcgaaccc
     5821 catgggacag atgacatcga gttcgctggc caggcgtgcg cgatagtgca cggccgcctc
     5881 ctcggaggct gccaaagcgc tcaggcgtgt ctccaggccg agcgccgact gcccacgcag
     5941 gtattcattg acgtcaatgc cgcacggcac cgggtaatcc ggcagacggg cctgaccgag
     6001 cgtgagctgc aaactgcagc ggcgtgcgat ctcaacggtg ttggccagcg cctcgggtac
     6061 atcctcaaac agccgctgca tctcggcggc agtgcgcaga tactgctgcg gcgtgtagcg
     6121 acggggcctt gcggcatccg ccagcagcgc accatcgtga atgcagactc tggcctcatg
     6181 cgactcaaaa tcctccgcgc gcaggaaccg cacgtcgttg gtcgcaacca ctggcaggtc
     6241 gtaccgcacg gccagcttga gcgcggcggg cagatagcgt tgctcctcgg gcctgccgat
     6301 gcgctgcaac tccaggtagt agcggtcttc gaacagcgag caccagaact cgagtgccac
     6361 ttcggcctgc gccatccgat cgtgcagcag cgctcggccc acatcgccct cgtgcgagcc
     6421 agacagcgca atcaggcccg cagtcgtctc gcgcgtcagc caggtgcgat caaccagcgg
     6481 cacggcggtc tgtgactgcc cctccaggaa cgcacggctg acaagccgcg ccaggttctg
     6541 atagcccacc aggtcctgac acagcagcgt cagacgcgac ggcccctgct tctcgtcgag
     6601 cccgcgcagc atcagatcca cgccgatgat tggctgcacg ccgttcgaca gcgcttcgcg
     6661 atagaacttc accatcgcga acaggttgcc ctgatcggtc agcgccaccg ccggcatacc
     6721 ggcggcagca accgcctgca tcaacccggg gactcgcacc acgctgtcga tcagcgagta
     6781 ctcggtgtgc aggcgcagat gtacgaaggt cgcagtcatt tcagcgatgt ctcactcctg
     6841 ggccgtttcc ggaaacatct cgagcgtccc aaaccgcagc tctcccgaca gccagctctt
     6901 gagcggattg aagctgcgac gatgaatcgc gcttggccct aatgcaacca gtgctttcac
     6961 atgggatcgt gtgcaatagc caaagtgttt gcccagcccg tagcccggat agcgctcgtc
     7021 ctggcggacc atccacgcgt cgcgataagt cttggcgatg atcgaggcag cgctgacgca
     7081 ggccaccgaa gcatccccgc cgacgacggt tcgaatgacg cattctgcaa aacaatcatg
     7141 cagctgcggc gcacagttac cgtcgattat gatttgcgtt ggacgcgctg acagccccag
     7201 cagagcgcgc ctcatcgcca gcagcgtcgc ctgcagaata ttgatcgcat cgatttcatc
     7261 gtgatcggac tgcgccaccg cccaggcgag cgcgcgctca cggatgcgct cggccagcac
     7321 ttcgcgccgc tcggcgctga gctgtttgga atcacgcagg ccgcgaatcc gttgcgcggg
     7381 gtcaaggatt accgcggcgg cgaccacggg tccggcaaga ggtccgcgcc cggcttcgtc
     7441 cacccctgcg atcagttcgc gcaaccttgc ggccctcctt cagcgttccg aagcttaaga
     7501 tatattttag gatatatctt cgctcgcgcc gattttcttc ggcaccggat ctggcggcat
     7561 gtggcacccc tagcgcgaag acggcgcgcg ctcgcgcagc attctcagaa tctcatcggc
     7621 ggcgcgctcc gcgccaccgg cctgcaacgc acgatgcacc tgcaggaatt tctgctcaag
     7681 gtagtgccgg cgtggaacgt cacgcaacgt ctgttcgacc gccgcgctca ggttggcgga
     7741 cgtcaccgcc tcctgcagaa attcgggcgc cacctgctcg cccgccagca gattcggcag
     7801 cgagaaatac ttcagccgca ccagccgcag ggtgcgggcc aggaacgccg tgagggaacc
     7861 aaagcgatag gccaccacca tcgggcaacg gcacagcaac gcctgcaatg tcgccgtgcc
     7921 cgaggcgatc agtgcggcat cggccgccgt caacgcttca cgcgcctgcc cgtccagcag
     7981 cttcaacggc aactcaccgg cgccgggcgt tgctgctagt gcgcggctga actcggcacg
     8041 cgcggcggcg ttcgccatcg gcgccaccag acgcagctgc gggatgcgcc gatgcagctc
     8101 gagcgcggcg gcaatgaagg tcgagccgag gtggcgcacc tcgctcaggc ggcttcctgg
     8161 gagcagcgcg agcaccggtt gcgccggatc ggccggcagt cccaggctcg cgcgggcggc
     8221 ggcgcggtcc gacgtgagcg gaatctgatc ggccagcgga tggccgacga actgcgctcg
     8281 caccgcatgg tcgcggtaga acgccggctc gaacggcagc aggcacaaga tcagatcgca
     8341 gcgccgcgcg atgctgcgta cccgtccctg acgccacgcc cagatctgcg gactgacata
     8401 ttgaacggta gcgatgccct gctgcttgaa gcgtccggcc agccccaggt tgaacgccgg
     8461 cgagtcgatg ccaatgaaca cgtcgggctt ccacgccgtg atacgctcaa tcagcatcga
     8521 gcgcagacgc agcagccgcg gcagctgcaa cagcggatcg ataaacccca tgacggccag
     8581 ctcatgggca tcggccagcg tctcgcaccc ggcctcgcgc atggcaggcc cggtgacgcc
     8641 gcgaacctcg agcgttgcca cccgctcgcg cagcgcgcgg atcagcgcac cgcccagcgt
     8701 gtcgccggag gtctcaccgg caacgatcgc aatcttcagg gccgatgcgg cgggcatccc
     8761 ttttgcggtc agcggatcag gctgcgttcc gagttctcaa tgaagctcag caacaccttc
     8821 aactcgggct gcgaggcggc aagctcgttg agctgcttca cggcggtatc gagcggcaga
     8881 tcgctgcgat acaggatccg ataggcgttc ttcaggttgc ggatctgttc ggccgtaaag
     8941 ccgcgccgct tgagtccttc gatgttgatg gagtgaggaa ccgccggatt gccgacagcc
     9001 atgacgtagg gcggcacgtc cttggtgcaa ccggcgtggt gggcgatgaa agcatgcgca
     9061 ccaatcctga cgtactgatg aacagcggcg tagccgctca gaatcgccca atcacccagg
     9121 tggacgtggc caccaatcat cgccaggttg gacatcacga cgttgttgcc gatcacacag
     9181 tcgtgcgcga catgggtata cgccatgagc aggttatcac tgccgatgca cgtgactgcg
     9241 cgatcgtgga ccgtaccgag gctgatggtg cagtactcgc gaatgctgtt gcgatcgccg
     9301 atttccagtc gcgtgggctc gcctcggtgc ttgagatcct gcggcgcctc gccgagcgac
     9361 gagaactgga agattctgtt gtcctgcccg atcgtggtgt gcccgttgat gaccacgtgt
     9421 ggcgcgacca cggtgcgcgc cccgatcacg acgtcggcgc caatgatgct gaagggacca
     9481 atctgtgcgt cgggagcgat ctttgcgccc gacgctacga tcgcctgcgc actgatcatg
     9541 gcagctccgg caccgccgca gcgccaccgc tggatttcag atccggcgcg atcatgatca
     9601 gggcgttggc gacttcttcg tcgcccacct ccgcgcgcgt cgccagccgc cagatgccct
     9661 tgaagttgcg ctcgaagttg gccgtcagaa ttaactgatc gcccggcgcg accggtttgc
     9721 gaaaccgcgc tttgtcgatg cccacaaaat aaaaacgcgt gttgtcgtcg ggaaaggcat
     9781 tggctgtcag gaatgccagc aggccggcga cctgcgccag cgcctcaatg atcatcacgc
     9841 ccggcatcaa tggccggttg gggaaatgcc cggtgaagta cggttcgttg taggtaacgt
     9901 tcttcagcgc ccggatgctc tgtcccgcga catagtccag tactcgatcc accagcagga
     9961 atggatagcg atgcggcaat cgcagcatga cgctattgat gtcgaggttg gtcgcgtcat
    10021 tcattctgtg tcgtcctttc gcagccgttg ttcgcgccgc actccgagct tcatccgtgc
    10081 cacgatccga tgccaggtcc tcaccggctc gagccctacc accccggaat agattccggg
    10141 ctgtttgatc gagtgcggta cagccgactt accagtgatg accacatcat cgcaaatctg
    10201 aatatggcct gcgaatccta catggccgcc gatcatgcag cgcgcgccga tgcgggtact
    10261 gcccgccacc ccggtgcagc ccgcgatcgc ggtgtgctca ccgacgaaga cgttgtggcc
    10321 gatctgtatc tggttgtcga gcttcacgcc gctgctgatc acggtgtcgt ccatggcgcc
    10381 gcgatcgatc gtggtgttgg cgccaatctc cacgtcatcg ccgatgcgca cactgccgac
    10441 ctgcggcacc ttgatccagc cgtcgctgtc gcgcgccagg ccgaaaccat cggcgccgat
    10501 cacgacgccc gggtggatca gcacccgctc gccgatcatc acatcgtgct cgatggtgac
    10561 gcgggagacc agacggccat ctgcgccgat gcgagcgttg cggccgacca ggcaggtcgc
    10621 ccccaccaga cagcgcgcgc cgatgcgggc accggcaccg atcacgctat aagggccaat
    10681 ctctgcgctg ggatccacgc tcgcagagca atcgacgatc gcgctcggat gtatccctgg
    10741 atgcggcacg ggggagggat agagcagcgc tgcgatgcgc gcgaaagtgg cgtgcggatt
    10801 gcgatcgagc agcgccgcga ccgcagagcc gtcggcgctg cgcggatcga gcaccacggc
    10861 cgacgcccgg gtactggcca gcagcggcgt cagccttgga ttggccagat aactgatggc
    10921 gccggggcct gccgactcca gtggcgcgat gtgatcgaca cgggtgtccg gatccccgcg
    10981 caattcgcag ccgaagcgca ccgcgatctc gcccagcgcg atactcatgg cgaagcgccc
    11041 gtcttggctc gcctaagact atttgccgtt cgagggcgag gtactcttgg gtggggtcgc
    11101 agatccggtg ctcggtgcgc gcggtccgcg agcctgcagg gcactcagaa tctgaggagt
    11161 aatgtcgagg gacgggcctg cccagatcac cccatcggcc agcaccaggt cgaacttctg
    11221 cgtctgcgca taggcctgca cttcgtcgac cagcgtcttc tgcagcttcg acagctcttc
    11281 gttctgccgt gagttgaact catcctgcag atcgttcacc ttcagcgaca attggcgatt
    11341 gccttcgcgc agctccttct ccgcctgatt gcgctgctcg ggggtcatcg tcgccgagtc
    11401 acgctgcaga gattcttcct tggttttcag ggcct
//
LOCUS       DATMRM010000309         6618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1424202, whole genome shotgun sequence.
ACCESSION   DATMRM010000309 DATMRM010000000
VERSION     DATMRM010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1424202"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VNZ06_09185"
     CDS             <1..293
                     /locus_tag="VNZ06_09185"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWW20965.1"
                     /translation="AGQSNYSAAKAGVESMGVVWAKELARYGIRVASVAPGFTQTEIL
                     AAMRPEVLEKLTAPVPLKRLGKPEEIASAVQFIFENDFFTGRCLDIDGGLRL"
     gene            488..823
                     /locus_tag="VNZ06_09190"
     CDS             488..823
                     /locus_tag="VNZ06_09190"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HWW20966.1"
                     /translation="MTEFDPKSVLDAYRNAFAPVIKAQQESVKAIDRAGRYQYALAGD
                     YLEWSLAQAKAAVSAQSPAEFVSKQVELASVFGEKLRTRVQEFVTLTTEATSKVVDAT
                     KVDVTKKAA"
     gene            974..2689
                     /locus_tag="VNZ06_09195"
     CDS             974..2689
                     /locus_tag="VNZ06_09195"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWW20967.1"
                     /translation="METTVPQQAELERTMRAQLATITGGLAPDVYVNAWWDWYLNLAK
                     EPPKQLQIMQDALAKATDSWNFALKASSGQAMSPDPADPRYANSAWSQWPFNIYAHSY
                     NNLADWWQQAWSGVAGVASKNERVLGFMARTTLETLSPSHYLATNPELLEQTRLEAGQ
                     NLVRGFQNWLDDTERTFEHKAPAGTDRFVVGRDVAATAGKIVMRNDLVELIQYSPSTE
                     SVYAEPILLVPAWIMKYYILDLSAKHSLVQYLVSQGHTVFIVSWKNPNASDRNLGMDD
                     YLKLGIRAPLNAVANIVPDRKIHAVGYCIGGTLLSIGAAVLAAEGDQRLASLTLLAAQ
                     QDFSEPGELSLFISPSQLDMLEAVMQKAGVLKSEQMGGAFAMLRARDLLWTPAVNAYL
                     RGQRDSPNDMMAWNADGTRMPYRMHSEYLRGLYLNNALAKGEFLAAGKLVDLSKITVP
                     MFMIGTETDHVAPWQSVYKARGLTRSSDYTFLLTSGGHNAGIVSGPVHPKRRYRIRSW
                     NNTTDTLPAKDWFDTTPAQPGSWWPVWQQWLVRHSSEKVPARQPGSQAYPPLDEAPGS
                     YVLQK"
     gene            complement(2671..3978)
                     /gene="guaD"
                     /locus_tag="VNZ06_09200"
     CDS             complement(2671..3978)
                     /gene="guaD"
                     /locus_tag="VNZ06_09200"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HWW20968.1"
                     /translation="MSDPTRMAHRGSLLHFRSDPGAHDDPNAYEYWEQGALIVNEGRV
                     ERVGPAQPLLCAEPSLPVTDHGRCLMLPGFIDTHIHFPQIDIIAAGGRHLLEWLEHYT
                     YPAEQRFADASYARGVAQVFLDELLRHGTTTAQVLGTVHKGAIDSFFEVAAVRRLRMI
                     AGKTLMDRMAPEGLRDDAVSGEREMRELIDAWHGRGRLHYAITPRFALTSTPEQLRSA
                     GALAREYPDVFVHSHLAENRQEVEQVRRAYPERRSYLDVYDHVGLLRERSTFAHCIYL
                     DHEDRARMAASGAGAAFCPSSNLYLGSGLFDLAASDAAGMRIALATDVGGGSSFSMLH
                     TMADAYRVAHLTGQHLTPLRAFYLATLAGARMLKLSDRIGSFAVGNEADFIVLQPQAT
                     ALLARRMEQSRSLSERLLLYMMLGDDRAIARTYVLGQPYFCST"
     gene            complement(3994..4920)
                     /locus_tag="VNZ06_09205"
     CDS             complement(3994..4920)
                     /locus_tag="VNZ06_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWW20969.1"
                     /translation="MNIDTWVTIAASAVGAATPLVYAALGELIVERTGVLNLGVEGMM
                     LVGAVTGFAVGVSSGSLALGYLAAAVAGMLIALLFGFLSLSLQTNQVATGLALTLFGV
                     GLSAFAGRRFVGLPISRVPLLHIPGLSDLPVIGPLLFRYDLLVYLSVGLCALIAWVLR
                     RTRLGLRIRAVGEQPAVAHALGASVNRIRYVAVLFGGASSGVAGAYLSTAVTPMWTEG
                     MSAGRGWIALALVVFGTWKPWRVLFGAYLFGTVTVLQLYAQGLGVHIPSEFLSMLPYL
                     ATIVVLVLICRDARTILLNQPASLGRSFHPGS"
     gene            complement(4917..6023)
                     /locus_tag="VNZ06_09210"
     CDS             complement(4917..6023)
                     /locus_tag="VNZ06_09210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWW20970.1"
                     /translation="MSSKQSARMREARRWPWRLERRARASTVMSLSASPLAAVAMLLT
                     GFVALSALGTDPIKAFNVFFVEPVSSVYGVGELLLKTTPLLLCALGLAVGYRSNVWNI
                     GAEGQFTLGAIAGGGVGLYCSQWLGSACMPAMLVSGALGGMAWAAIPAFLRVRFRTSE
                     IFVSLMLVYIAQLLLSYLVHGPWRDPAGQNFPQSPPFSDHATLAALIDGARVNAGFLI
                     ALALAALSWWFGSMTAAGFRMRVGGLAPAAAAYAGISERRNVWLAMMIGGATAGIAGV
                     CEVAGPVGMLQPVISPGYGFAAIIVAFVGRLHPVGIVLASLLMSALYLGGESAQVELQ
                     LPASVTGLFQGTLLFFLLAAELFIHFRLRSVARA"
     gene            complement(5992..>6618)
                     /locus_tag="VNZ06_09215"
     CDS             complement(5992..>6618)
                     /locus_tag="VNZ06_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20971.1"
                     /translation="AAISGETPIVVHEAVRIAGQAVGRCSAARRRELGLRFVPEERLG
                     RGVVPEMHLADNSVLTGAGAGLVRHGIVQHRLAREFARATIAQFSVKCDGELAPANSL
                     SGGNLQKFIVGREIRLAPRLMFVAQPTWGVDIGASMQIHQALIDLRNAGTAVLVISED
                     LDELYALCDRLAVLARGRLSAALPTAQLPVEKLGVWMTGEFEAERTDA"
BASE COUNT         1371 a         2194 c         1995 g         1058 t
ORIGIN      
        1 atgcgggcca gagcaactac agtgctgcca aggccggagt ggaatcgatg ggtgtcgtct
       61 gggcgaaaga gttggcgcgc tatggcattc gcgtggcatc ggtggctcca ggattcacgc
      121 agaccgagat tctcgcggcc atgcgtcccg aagtgctcga aaagctgacg gcgcccgtgc
      181 ccctcaagcg ccttgggaag ccggaagaga ttgcgagcgc ggttcagttc atcttcgaga
      241 acgatttctt caccggccgc tgcctggata tcgatggggg attgcggctc tgagtctcct
      301 gcgttgcaca tttatttcaa tcaggtgtct ctacgctgcc gctagcacaa aaatttatct
      361 atataaaaca aatatatata taaatataag ctcagcctga atgtctttgc gattcttctt
      421 gcagtgcata caaactgcgg tatactgtca acaccctgat cgcactggac tgctaaggag
      481 aactgacatg accgagtttg accccaagag cgttctggac gcctatcgca acgcattcgc
      541 gcccgtcatc aaggcgcagc aggaaagcgt gaaggccatt gaccgcgctg gccgctatca
      601 gtacgcgctg gcgggcgatt atctggaatg gagcctggcc caggccaaag ccgcggtgag
      661 cgcgcagagc ccggccgagt tcgtttccaa gcaagtggaa ctggcgagtg tgttcggcga
      721 gaagctgcgc acgcgcgttc aggagttcgt gaccctgacg accgaagcga cttcgaaggt
      781 cgtggacgcc accaaagtcg atgtgaccaa gaaagcggcg taaagccgcc cgttcggcgc
      841 gtttcccccc actcgcgccg aacccgagct gccgcagact cgcgagcagc tcttgaggcc
      901 ggaggcgggg gcgccacagg cgccctcgcc gacagtctgg ctagtttcct cctctacagg
      961 cacgctgcac ggtatggaaa cgaccgtccc ccagcaagct gagctcgagc gcacgatgcg
     1021 cgcgcaactg gcgaccatca ccggcggact cgcccccgac gtctatgtga acgcctggtg
     1081 ggattggtat ctgaatctgg cgaaggaacc gcccaagcaa ctgcagatca tgcaggatgc
     1141 tctagccaaa gcgacggaca gctggaactt tgcgctgaag gcatcgagcg ggcaggcgat
     1201 gtcgccggac ccggcagatc cgcgctacgc caacagcgcc tggtcgcagt ggccgttcaa
     1261 catttacgct cacagctaca acaacctcgc agactggtgg cagcaggcgt ggagcggtgt
     1321 cgcaggcgtg gcgtcgaaga acgagcgcgt gctgggtttc atggcgcgca ccacactcga
     1381 gacgctctcg cccagccact atctggccac caatccggag ctgctcgagc agacacgcct
     1441 tgaagccggc cagaacctgg tgcgtggatt tcagaactgg ctggacgaca ccgagcgcac
     1501 gttcgagcac aaggcgccgg ccggtaccga cagatttgtc gtcggccgcg acgtggccgc
     1561 cactgctggc aagatcgtca tgcgcaacga tctggtggaa ctcatccagt attcaccgag
     1621 caccgagtcg gtgtacgcgg aaccgattct gctggtgccc gcctggatca tgaagtacta
     1681 catcctggac ctgtcggcga agcattcgct ggtgcaatat ctggtgtcgc agggtcatac
     1741 cgtttttatc gtctcgtgga agaatccaaa cgcgagcgat cgcaacctcg gcatggacga
     1801 ttacctgaaa ctcggtattc gagccccctt gaacgcggtg gccaacatcg tgccggaccg
     1861 aaaaattcat gcggtcggct actgcatcgg tggcacgctg ctgtcgatcg gcgcggcggt
     1921 gctggccgct gaaggcgatc agcgactggc cagcctgacg ctgctcgccg cgcagcagga
     1981 cttcagtgag ccgggcgagc tgtcgctgtt catcagccca agtcagctcg acatgctgga
     2041 ggcggtgatg cagaaggccg gcgtgctgaa gagtgagcag atgggcggcg ctttcgcgat
     2101 gctgcgcgcg cgcgatctgc tatggacacc ggcggtcaac gcctatctgc gcggccagcg
     2161 cgattccccc aacgacatga tggcgtggaa cgccgatggc acacgcatgc cctaccgcat
     2221 gcacagcgag tatctgcgcg gcctgtatct caacaatgcg ctggccaagg gtgagttcct
     2281 ggccgcaggc aagctggtgg atctgtcgaa aatcaccgtg ccgatgttca tgatcggcac
     2341 cgaaaccgac cacgttgctc cctggcaatc cgtctacaaa gcgcgcggcc tcacgcgctc
     2401 gagcgactac acgttcctgc tgacttccgg cggccacaac gcgggtatcg tcagcgggcc
     2461 ggtgcatccg aaacggcgct accgtatccg tagctggaac aacacgactg ataccctgcc
     2521 ggcgaaggat tggttcgata cgacgccagc gcagccgggc tcatggtggc cggtgtggca
     2581 gcaatggctg gtgcgacact catcggagaa agtgcctgcg cgccaaccgg gctctcaagc
     2641 ctatccaccc ctggacgagg cgccgggcag ttacgtgctg cagaaatagg gttgcccgag
     2701 cacgtaagtg cgcgcgatcg cacgatcatc tcccaacatc atgtagagca gcagtcgctc
     2761 gctgagtgag cggctttgct ccatgcgtcg cgccagcagc gctgtcgcct gcggctgcag
     2821 cacgatgaag tctgcttcgt tgccgacggc gaaactgccg atccgatccg acaacttaag
     2881 catccgcgcg cccgccagcg tcgccagata gaacgcacgc aatggcgtga ggtgctgtcc
     2941 ggtgagatga gccacccggt acgcatccgc catggtgtgc agcatgctga agctactgcc
     3001 accgccgacg tcggtcgcca gcgcaatgcg catgccggca gcatcgctcg ccgccagatc
     3061 gaacagcccg ctgcccagat acaggttcga gctcggacag aaggctgcgc ctgcgccgct
     3121 cgccgccatg cgcgcgcgat cctcgtggtc gagataaatg caatgcgcga aggtcgagcg
     3181 ctcgcgcagc aggcccacgt gatcgtaaac gtccagatag ctgcggcgct cgggataggc
     3241 tcggcgcacc tgttcgactt cctgccggtt ttccgccagg tgactgtgaa cgaacacgtc
     3301 cggatattcg cgcgccagcg ccccggcact gcgcagttgc tccggcgttg aggtcagcgc
     3361 aaagcgtggc gtaatggcgt agtgcagccg cccgcggccg tgccaggcgt cgatgagttc
     3421 gcgcatctcg cgttcaccgg aaaccgcatc atcgcgcagg ccctccggcg ccatgcggtc
     3481 catcagtgtt ttgcccgcaa tcatccgcag ccgccgcacg gcagcgacct cgaagaaact
     3541 atcgatcgca cccttgtgca cggtacccag cacctgcgcc gtggtggtgc catggcgcag
     3601 aagttcatcc agaaaaacct gcgccacgcc tcgcgcatag ctggcatccg cgaaccgctg
     3661 ctcggcagga taggtgtagt gctccagcca ttcgagcagg tgccgaccgc ccgcggcgat
     3721 gatatcgatc tgcgggaaat gaatatgtgt gtcgatgaac ccgggcaaca tcaagcaacg
     3781 cccgtgatcg gtaaccggca gtgagggttc ggcgcacagc aatggctgcg cgggtccgac
     3841 gcgctcgacc cggccctcgt tcacgatcag cgcgccctgc tcccagtact cgtaggcatt
     3901 cgggtcatcg tgcgctcccg ggtcggaccg aaagtgcagc agcgagccgc gatgtgccat
     3961 tcgggtcggg tcgctcatga ggcgtccttg gcttcaggag ccggggtgga agctgcggcc
     4021 aagggatgcc ggctgattca gcaggatcgt cctggcatcg cggcagatca gcaccagcac
     4081 gacgatcgtc gccagatacg gcagcatcga caggaattcg ctcgggatgt ggacgcccag
     4141 gccctgcgca tagagttgca gcacggtcac cgtaccgaac agatacgcgc caaacaacac
     4201 tcgccagggc ttccaggttc cgaacacgac cagcgccagc gcaatccagc cgcggcccgc
     4261 actcatgccc tcggtccaca tgggtgtcac cgcagtggac aggtacgcac cggctacgcc
     4321 gctggaggca ccaccgaaca acaccgcaac gtaacggatg cgattcacgc tcgctccgag
     4381 cgcatgcgcg accgcgggct gctcaccgac cgcacgaatg cgaagtccca ggcgtgttcg
     4441 cctgagcacc caggcgatca gcgcacacag tccgaccgac aaatacacca gcagatcgta
     4501 gcgaaacagc agcggaccga tcactggcag gtcactgagc cctggaatgt gcagcagcgg
     4561 cacgcgcgag atcggcagtc ccacaaagcg ccggccggcg aacgcgctca ggccgacacc
     4621 gaataaggtc agcgccaggc cggtggcgac ctgattggtc tgcagactca agctcagaaa
     4681 tccaaacagc agcgcgatca gcatgccagc caccgccgca gccagatagc cgagcgccag
     4741 gctccccgaa ctgacaccca ccgcgaagcc ggtgacggcg cccaccagca tcatgccctc
     4801 gaccccgaga ttgagcacgc cggtacgctc cacgatcagc tcgccgagcg cggcatagac
     4861 cagcggtgtg gctgcaccca ccgcactggc cgcaatggtg acccaggtat caatattcat
     4921 gcgcgcgcca ccgaccgcag ccggaaatgg atgaacaatt ccgccgccag cagaaagaac
     4981 agcagcgtgc cctgaaacaa tccggtgacc gacgctggca gttgcaattc aacttgcgcc
     5041 gattctcctc ccagatacag cgccgacatc agcagcgacg ccagtacgat gcccaccgga
     5101 tgcagacgcc cgacgaacgc cacgatgatc gcggcaaagc cgtagcccgg tgaaatcacc
     5161 ggttgcagca taccgaccgg tcctgcgacc tcgcacacgc cagcaatgcc ggcggtcgct
     5221 ccgccgatca tcatcgccag ccacacgttg cgtcgctcgc tgatgccggc ataggccgcg
     5281 gccgcgggcg ccagaccacc gacccgcatg cgaaaacccg ccgccgtcat cgatccgaac
     5341 caccacgaca acgccgccag ggcgagcgcg atcaggaaac ccgcattgac gcgcgcgccg
     5401 tcgatcaacg ctgcgagtgt tgcgtgatcg gaaaacggcg gcgactgcgg aaagttctgt
     5461 cccgccggat cgcgccatgg cccatgcacc agataggaca gcagcagctg tgcgatatac
     5521 acgagcatca ggctgacaaa gatctcgctg gtgcgaaaac gcactcgcag aaacgccggt
     5581 atcgccgccc aggccattcc gcccaacgcg cccgacacca gcatcgcagg catgcaagca
     5641 ctgcccaacc attggctgca gtaaagaccc actccgcccc cggcgatggc ccccagcgtg
     5701 aactgccctt cggcaccgat attccagaca ttggagcgat agccaaccgc cagacccaac
     5761 gcacacagca gcagcggtgt cgtcttcagc agcagctcgc ccactccgta gacggaactg
     5821 accggctcaa cgaaaaacac attgaacgct ttgatcggat cggtgccgag cgcactcagc
     5881 gcgacaaatc cggtgagcaa catcgcgacc gcggcgagcg gtgatgcgct caggctcatc
     5941 acggtcgacg ccctggcgcg ccgctccagc cgccacggcc atcgacgcgc ctcacgcatc
     6001 cgtgcgctct gcttcgaact caccggtcat ccagacaccc agtttctcca ccggcagctg
     6061 cgcggtcggc aatgccgccg agagccggcc gcgcgccagt accgccaggc gatcgcagag
     6121 ggcgtagagc tcatcaaggt cttcggagat caccagcacc gcggtcccgg cattgcgcag
     6181 gtcgatcagt gcctgatgga tctgcatcga ggcgccaatg tccacacccc aggtcggctg
     6241 cgcgacgaac atgagccggg gtgcgagccg gatctcgcgt ccgacgatga acttctgcag
     6301 gttgccaccg gaaaggctgt tggctggcgc cagctcgcca tcgcatttga ccgagaactg
     6361 cgcgatggtc gcccgcgcaa actcgcgcgc cagacgatgc tgaacaatac catggcgcac
     6421 caggcccgct cccgctccgg tcagcacgct attgtcggcc aggtgcattt cgggcacgac
     6481 gccgcgaccc agacgctcct ccggcacgaa acgcaggccc agctcgcgcc gtcgcgcagc
     6541 agagcatcgg ccgaccgcct gccccgcaat gcgcaccgcc tcatgcacga cgatcggcgt
     6601 ctcaccggaa atggcggc
//
LOCUS       DATMRM010000310         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3567357, whole genome shotgun sequence.
ACCESSION   DATMRM010000310 DATMRM010000000
VERSION     DATMRM010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3567357"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VNZ06_09220"
     CDS             <1..399
                     /locus_tag="VNZ06_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20972.1"
                     /translation="SIGAIGLDPSNPKNVWVGTGESWTRNSVSIGDGIYKSTDAGETW
                     THMGLSDSERISQIIVDPRSADTVYACVPGRLWSDSPQRGLYKTTDGGTSWELVLKGS
                     NLSTGCGSIALDLRAPQVLFASLWDFRRKG"
     gene            375..>2558
                     /locus_tag="VNZ06_09225"
     CDS             375..>2558
                     /locus_tag="VNZ06_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20973.1"
                     /translation="MGLPAQGLTFRSGGDGPSAPSGSGLYRSADGGKSWTEITPQANK
                     GFPPKPYGRIALALAPSDSRTLYAFVESTDSALLFSHDGGGTWERGDKSQWMVWRPFY
                     FAKLIVDPKDANRLYKTDGYLIMSEDGGKSFSNIGGFTGMHGDVHDVFIEATNPKHVF
                     AGDDGGLWISYDGANKWWKTDNLPISQFYHVSLDNADPYHVYGGLQDNSSWVGDSAYP
                     GGITSSRWENMFNGDGFWMFADTSDPDYVFAEYQGGSIGRVKMRTHETRDIQPKLGAA
                     DLKRYTKLRFNWNTPIALSPHEPGTVYIGAQFLFRSRDHGQTWERISPDLTSNDPQKQ
                     KQEQSGGITVDNSAAEMHTTVYSISESPVAAGLIWVGTDDGNLQLTRDGGQHWDNVVG
                     NVPGVARNAWVSWVQASAFAPGSAYVAFDRHTFGDMAPYVYATTDFGKSWRPLLNSQD
                     SKGVRGYAHVIKEDTVDSQLLFLGTEFGLWISVDGGAHWAQFKGGHLPAVAVRDLAIQ
                     PRDNDLVLATHGRGIWIVDDITPLRQLTPQLTSQDAAFVSARPAQQRIEANGGWANGA
                     AAFVGDNPAGGAVITYYQRTRHLFGKLRIEVLDSSGELIDELSASTRRGLNRVVWTMH
                     RHPPHVPPAAQLAQAGTQGPRVLPGTYVIRLHKNDRVYENQATIGLDTRVTWSLADRK
                     AQYEAAMKVYALFNDESALFARIAGLREQVAEAGKSRPKGDALLRR"
BASE COUNT          487 a          826 c          805 g          440 t
ORIGIN      
        1 tcgatcggag cgatcgggct cgatccctcc aatccgaaga acgtctgggt gggcaccggc
       61 gagagctgga cacgcaacag cgtgtcgatc ggcgatggca tctacaagtc cacggacgcc
      121 ggcgagacct ggacgcatat gggcctgtcc gactccgagc gcatctccca gatcattgtc
      181 gacccgcgct cggccgatac cgtctatgcc tgcgtgccag gcaggctgtg gagcgactcg
      241 ccccagcgcg gtctctacaa gactaccgac ggcggcacca gctgggagct ggtactcaag
      301 ggttccaatc tgtcgaccgg ctgcggcagt atcgcgctcg acctacgcgc cccgcaggtg
      361 ctgttcgcaa gcctgtggga cttccggcgc aagggctgac gtttcgctcc ggcggcgacg
      421 ggccgagcgc gccgtccggc agcggcctgt atcgcagtgc ggatggcggg aagagctgga
      481 ccgaaatcac tccgcaagcc aacaaaggct tcccgccgaa gccttacggt cgcatcgcgc
      541 tcgcattggc gcccagcgat tccaggacac tatacgcctt cgtcgagtcg accgactcgg
      601 cgctattgtt ctcgcatgac ggcggcggca cctgggaacg cggtgacaag agccaatgga
      661 tggtgtggcg accgttctac ttcgccaaac tcatcgtcga tccaaaggat gccaatcgcc
      721 tctacaagac cgacggctat ctcatcatga gcgaggacgg tggcaagagc ttcagcaaca
      781 tcggcgggtt caccggcatg cacggcgatg tgcacgacgt cttcatcgag gcgaccaacc
      841 cgaaacacgt gtttgccgga gacgacggcg gactgtggat ctcctacgac ggcgccaaca
      901 agtggtggaa gaccgataat ctgccgatct cgcagttcta ccacgtcagc ctcgacaacg
      961 ccgaccccta ccacgtctat ggcggcctgc aggacaacag ctcctgggta ggtgactccg
     1021 cctatccggg cggcatcacc agttcccgct gggaaaacat gttcaacggc gacggcttct
     1081 ggatgttcgc cgacacttcc gatcctgact acgtctttgc cgaataccag ggtggcagca
     1141 tcggccgcgt caagatgcgt acccacgaga cgcgggacat ccagccgaag ctcggcgcag
     1201 ccgatctcaa gcgctacacc aagctgcggt tcaactggaa cacccccatt gcgctgtccc
     1261 cgcatgagcc gggcacggtt tacatcggcg cgcagtttct gttccgctcg cgcgatcacg
     1321 gccagacctg ggagcggatc tcaccggacc tgaccagcaa cgatccgcaa aagcagaagc
     1381 aggaacagtc cggaggtatc accgtcgaca attccgcggc ggagatgcac accacggtct
     1441 attcgatcag cgagtcgccg gtggcggccg ggctcatctg ggtcggtacc gacgacggta
     1501 acctgcagtt gacccgcgat ggcggccagc attgggacaa tgtcgtcggc aatgtgccgg
     1561 gtgttgccag gaacgcctgg gtaagctggg tgcaggcgag cgcattcgcg cccggcagcg
     1621 cctatgtcgc ttttgatcgg cacactttcg gcgacatggc gccgtacgtt tatgcgacga
     1681 ccgactttgg aaagtcctgg aggccgctgc tcaactcgca ggattccaag ggcgtacgcg
     1741 gctacgccca cgtcatcaag gaagacaccg tcgattcgca gctgctgttt ctcggcaccg
     1801 agtttggact gtggatctcg gtcgacggcg gcgcgcattg ggcgcagttc aagggtgggc
     1861 accttccggc agtggcggtg cgtgatcttg cgattcagcc tcgcgacaat gacctcgtgc
     1921 tcgcgacaca tggccgcggc atctggatcg tcgatgacat cacgccgctg cgccagctca
     1981 cgccgcagct gacctcgcag gacgcggcct tcgtgtccgc gcgccccgca cagcagcgca
     2041 tcgaggccaa tggtggttgg gctaacggtg ccgccgcctt cgtaggcgac aatccggccg
     2101 gcggcgccgt gattacctat taccagcgca cccgccacct gttcggcaag ctcaggatcg
     2161 aagtgctgga ctcaagcggg gagctgatcg acgagctgtc cgcgagcacc cgccgcgggc
     2221 tgaatcgcgt ggtgtggacc atgcaccggc acccgccgca tgtcccgccc gccgcgcagc
     2281 ttgcgcaggc cggcacgcag gggccgcgtg tgctgccggg aacctatgtt attcgccttc
     2341 acaagaacga tagagtctat gagaaccaag ccaccatagg cctcgacact cgcgtcacgt
     2401 ggagcctggc ggatcgcaag gcgcagtatg aggcggcgat gaaggtttac gcgctgttca
     2461 acgatgagtc ggcactgttc gcccgcatcg ccggcctgcg cgagcaggtt gcggaggcag
     2521 gcaagagccg gccgaaaggt gacgccctgc ttcgccgg
//
LOCUS       DATMRM010000311        14736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1749830, whole genome shotgun sequence.
ACCESSION   DATMRM010000311 DATMRM010000000
VERSION     DATMRM010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14736
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749830"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VNZ06_09230"
     CDS             complement(<1..864)
                     /locus_tag="VNZ06_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20974.1"
                     /translation="MANPLRSGTDCAAWHELESLAPTFASAPTRELFARDAQRFQHCS
                     AEAAGLVYDYSRQRVDASVVNCLVTLADQLGLRSRIEAMFRGDAINSTENRAVLHTAL
                     RRSSTAALMVGSEDVSTLVRNERDRMLGFAEDVRQGIVRSSTDQSFNLVVNIGIGGSD
                     LGPAMAVEALRQFTSGAPRVAFVSNVDGCQLADLIASADPERTLFMICSKTFTTLETQ
                     TNAKVARDWILQRLGAKGLPQHFAAVSVNAKAMDEFGVHPDYRFAMWDWVGGRYSLWS
                     SIGVSLAIAIGR"
     gene            complement(921..1670)
                     /gene="dnaQ"
                     /locus_tag="VNZ06_09235"
     CDS             complement(921..1670)
                     /gene="dnaQ"
                     /locus_tag="VNZ06_09235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HWW20975.1"
                     /translation="MRQVVLDTETTGLESKLGHRIIEVGCVELINRRATGRIFHRYLN
                     PDREIDYGAVGVHGITRERLESAPRFAAIADELLAFVADSELIIHNAEFDVEFLDMEL
                     SRLGSATLPASLRSVCRVLDTLTLARARHPGQRNNLDALCRRYEIDNSHRELHGALLD
                     ARILADVYLAMTSGQNLLALDESGPEAGLGAHAALSAISVRPAEPLRVIRASAQECAA
                     HGELTRLLQKASGGKCIWLALAPPNPLSAAT"
     gene            complement(1682..2143)
                     /gene="rnhA"
                     /locus_tag="VNZ06_09240"
     CDS             complement(1682..2143)
                     /gene="rnhA"
                     /locus_tag="VNZ06_09240"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HWW20976.1"
                     /translation="MSQVVEIYTDGACRGNPGVGGWAAVLSAGGREKEISGAETLTTN
                     NRMELQAVISALQALKRAVEVRLYTDSQYVRRGVLEWLPQWKARGWKTADKKPVKNQD
                     LWQVLDGEVAKHRIEWHWVPGHSGIPGNERCDALANAAIDALLAVDSRQPS"
     gene            complement(2140..2862)
                     /locus_tag="VNZ06_09245"
     CDS             complement(2140..2862)
                     /locus_tag="VNZ06_09245"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWW20977.1"
                     /translation="MADPLPVLQQWQTESLGRALIQAEAQLLSTTFDDVFGLELLQVG
                     AWGVGRELLAPARIRRQSVVADVSQGTARGDIVASLAHLPISNATVDAVLLPHTLELA
                     ADPYAVLREADRVLGAEGQLIVLGFRPMGFWGLRAAASQLGFPPGLRRTLSASRVRDW
                     LGLLSYEILSVHPYLFRTPRPLRGSVEAAIPGILRRGWLYPWPAAAYLIKARKRVYTM
                     TPIRPRLRERVRVFGGLAEPSR"
     gene            3007..4758
                     /locus_tag="VNZ06_09250"
     CDS             3007..4758
                     /locus_tag="VNZ06_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWW20978.1"
                     /translation="MRLQPIWKASIAALVLALSACAHQPPRAPQSTAQLDIPFQPASA
                     APATDLQPPAAAPSAQVEQADSAPALEASVSQSVSEVPAAAGPVDSEGLPGPPPDSVV
                     VAREPDLFARLRSGFQLSDVQEPAVSSQLNWFANHPDFLERTWGRASLWLYYIVGQIE
                     QRKMPAELALLPVIESAFEPYAYSRARAAGLWQFISDTGHRFGLKQDYWYDGRRDPIE
                     ATRAALDYLQELHDEFNGDWLLAVAAYNCGELAVERAIERNQRLGKPTDFWHLKLPTE
                     TRGYVPELLAMRRLVSNPERFGLEFSPIPNEPYFITIQTGGQIDLKVVAQMAGVTADD
                     LYELNPAFHRWATDPTGPYHLLVPADSAEGLQQAVLQLTPEQRMSVEHYTVRHGDTIA
                     SIATHFRTRPEVIRELNDLDANELPVIDTSLRVPSPDIQLPEKAARAALLADAPPGRL
                     RRHGGKPRIHVVRRGDTLYALAQRLGTDPQTLAQINGLNPGDRLRAGQKLRMSSSNAL
                     VASAPSRTSTKTASSGSAADGARRMTYVVRRGDTLYSIARLLQVTVSDLADWNGVSGN
                     HSLKPGQILIAFVKSRT"
     gene            4777..5568
                     /locus_tag="VNZ06_09255"
     CDS             4777..5568
                     /locus_tag="VNZ06_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HWW20979.1"
                     /translation="MGILSGKRALIVGVATERSIAWGIAQAMHTHGAQLAFTYANDTF
                     KERVEPLAASIGSPLVMPLDVQLDEQIDSAFALLQREWQQIDILVHAVAFAPREALSG
                     RFTANTSREAFRIAHDVSSYSFTALARGAAPLMQGRAGALLTLSYLGAERSIPNYNVM
                     GLAKASLEANVRFLAADLGPQNIRVNAISAGPIKTLAAAGIGGFRKMLGQVAQIAPMR
                     RNVTLEDVGNAATFLCSDMAAGITGEILYVDNGFSNVGMSFDNEG"
     gene            complement(5589..6728)
                     /locus_tag="VNZ06_09260"
     CDS             complement(5589..6728)
                     /locus_tag="VNZ06_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20980.1"
                     /translation="MKETITTAVLLVLATGPALADITTQESMALDAAGIVKLHGTTTE
                     MTADDRQRRDSEFHCEGFMSLLCGNAKSGEIIRLDKDLTWQLRPDKKTYLETAFPTPE
                     ERAMAQQKMQETLDKMKQCQQQQAPRGAPSKGDTSNCDLSPPKVDMRTTDEHATIAGH
                     DARKSSVKMSQTCTDKKTGDICEMVYGFDIWMTQDPLEGTADKRAFQKAYLTKMGLDA
                     NSPQIKGIAQQFMAAYSGTLKDMQAKAASLKGYPLRTTFRLIMGGDNCSKSKQSDSDN
                     GAGAGGGGLSGLAANAGGKLLGGLFAKKNAAGANDPSQGAIAANAPPPLPDSYVQVIA
                     FTIETSAVSTAAVSADQFELPAGWTREYPKAAKNSEYSCPNNKDK"
     gene            6863..8878
                     /locus_tag="VNZ06_09265"
     CDS             6863..8878
                     /locus_tag="VNZ06_09265"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HWW20981.1"
                     /translation="MLMDTHRASKPWRVGRGVALAAAVLLIALVWFMALFDWNHLRGA
                     ISGLASAHLHRQVRMDHLSVRVWQRNPSVAIEHLSVSNPSWAAGNMVEVGQLTFAIEP
                     WPLLRGRLVLARLQIDRPQVVLLRDSGNRANWDFSDDQMKKKQADKPGPPAQLPVIHR
                     FTLNGGTLKVDDAIRKLRFDGTLSANEGATRALSHAFELKGQGQLNGKAFELTFGGSS
                     LFNLQLDEPYDYDAEIHAGPLQATARGVIDKPFDLGHLSANLDFKGENLAALYYLTGL
                     ALPFTPPFHLSGELRRDGMMYRLRKLQGTVGDSDLHGELSVEAAGTRPRLTAELISHA
                     LDLADLAPSVGAGVPNDNTDAKSDTQAPRAQADDKLLPTYQFDFDRLRSMDASVELRA
                     DSVKTQKVPITGVNLKLNLERGVLTLDPADLTLPQGKLGGAIRINAQNAVADTSIDLR
                     LSNVNLSELKSAKMSEAPLDGDLLSRVLLEGSGNSVHEIFASSHGEIVAVIPHGEMRQ
                     AFAELTGINAARALGLLLTGSRKEDGIRCGIAAFSVDHGKATAEPLVFDTDTVAVTGS
                     GGFDFNTEDLDLRLKGQSKKFEPLHVRAPITVRGTLGKPSIGLDPKSLAAQGGAAAAL
                     GVLATPLAAVAAFIDPGLAKNQDCSRLLSSAPEKAAEQPGAPQLAPK"
     gene            complement(8894..10813)
                     /locus_tag="VNZ06_09270"
     CDS             complement(8894..10813)
                     /locus_tag="VNZ06_09270"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWW20982.1"
                     /translation="MLQNLGDKLKSQRWLATLVLVPLALIFAIWGAYGVVNMSFGAPD
                     YALRVNGERISNQTINRAWQERQAQYQQMLNGSEMTDEMKSTLQQKLVDEFIRNTVLR
                     QRAEEDGYRATPQEIAEYMHSEPAFQVDGKYDPRAASIAIARIGMTDRAFLNERSQSM
                     AVGQLNSAIENSEFMTPAELDRVYALENEQRELRYAVLPADHFVSGLKIDDARIKAWY
                     DAHSADYMTPESVKLQYAELRLDALTPQVSVKPEDLQAYYEKVKARYTEPEKRHAHHI
                     LISIADPKDAKAEAAALARANQIEADLKSGKDFAELAKKNSADPGSAAQGGDLGWADQ
                     TAFVGPFADALFSMQPGQISDPVKTQFGYHIIRLDEIRPAHVRGLDEVRAELETEYRR
                     DQAAELFGDRQEKLQQRVESGSGDLDAIAKQFDLPTGEIQDFTRTTGGAPLGGSPAML
                     AAVFNDDMLAGGKIGGPVAISDDHLVIFRVLEHRAPAAQSIASVRDEIIAAVKKSEST
                     AAAKSAADNAVKQLDGGTPFDQVVRGLGISSAPAAYVGRNDPQIPMQVRQEAFQVPHP
                     NGKPVDRAVALDNGGAAVLAVSAVKPGAGGTNPQNDTQLVIQFLRRDRDASMNAYLAE
                     LQRRANVKRNPKIFE"
     gene            complement(10823..10899)
                     /locus_tag="VNZ06_09275"
     tRNA            complement(10823..10899)
                     /locus_tag="VNZ06_09275"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10863..10865),aa:Asp,seq:gtc)
     gene            complement(11028..11786)
                     /gene="lpxA"
                     /locus_tag="VNZ06_09280"
     CDS             complement(11028..11786)
                     /gene="lpxA"
                     /locus_tag="VNZ06_09280"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HWW20983.1"
                     /translation="MPSGHIHPTAIVEPGARLGTGCIVQAYAFVSRHCELGEAVVVHP
                     FALLGGDPQDLSFRGEVSSGVRIGARTIIREHVTISRATQADTCTEIGADCFLMASCH
                     VAHDCRIGDRVVIANAVLLAGHVHVDERVFLGGGAVVHQFCRIGENAMIGGGARISQD
                     VPPFCMATERSALIGLNAVGLRRRGLKAAALAELKRAYRALDVPVGNLREIAAALLGR
                     GDFVSPEAQTFLKFFCGGRRQFVRSRSRTTALEE"
     gene            11856..12158
                     /locus_tag="VNZ06_09285"
     CDS             11856..12158
                     /locus_tag="VNZ06_09285"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWW20984.1"
                     /translation="MRRIRRPSAHTLSLLEALSQRPRAWQYGYELSQITQLKSGTLYP
                     ILMRLSDRGFLESRWQPAQQPGRPPRHVYRLTSSGAAFAREAFARQEGEATRGEFA"
     gene            12155..12763
                     /locus_tag="VNZ06_09290"
     CDS             12155..12763
                     /locus_tag="VNZ06_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW20985.1"
                     /translation="MSAQHPLSRQVARALLAHAARVSPAGRRGWFVAMASELDHLPHG
                     ASVLQWALGCLLVAYTERIMDRSLTHLPRWLLATEMAVCLVPLTWLFVAVIMVTAQGH
                     MTLVWGLKYASAALVGPLGLALAARILFVRSRALGRAAATLLALLAAWTFIAELGQSL
                     DTGLPVSTWWRDFVLIAVLPAVAVSHLLWINADRRARALRAH"
     gene            complement(12795..12870)
                     /locus_tag="VNZ06_09295"
     tRNA            complement(12795..12870)
                     /locus_tag="VNZ06_09295"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12835..12837),aa:Val,seq:tac)
     gene            complement(12940..14733)
                     /gene="lon"
                     /locus_tag="VNZ06_09300"
     CDS             complement(12940..14733)
                     /gene="lon"
                     /locus_tag="VNZ06_09300"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:NF008053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HWW20986.1"
                     /translation="MRGRVKAQMEKSQREYYLNEQMKAIQKELGEMEDGTNEIAELEQ
                     KVVKAGMSKEAREKATAEINKLKMMSPMSAEATVVRNYVDWLIKAPWKKRTRLLKNIV
                     NAQKVLDEDHYGLDKVKERIVEYLAVLQRVEKIKGPILCLVGPPGVGKTSLGQSMARA
                     TNRKFVRMSLGGVRDEAEIRGHRRTYIGSMPGKIIQNLSKTGVHNPLFLLDEVDKMAM
                     DFRGDPSSALLEVLDPEQNATFADHYLEVDFDLSETMFVCTANSLNIPAPLLDRMEVI
                     RLPGYTEDEKLNIARRYLVPKQTKQNGLRDGELKISDGSILDIVRHYTREAGVRNLER
                     EIAKISRKAVKQLLLDKADKKSGSVAVTPRNLDKYLGVKRFRYGKAEEMNRVGQVTGL
                     AWTEVGGELLTIEAAVVPGKGKLLHTGQLGEVMQESIQAAMSVVRSRAAVLGLDVDFY
                     QKNDVHIHMPEGATPKDGPSAGIGMCTALVSALTRIPVRSDVAMTGEITLRGEVLPIG
                     GLKEKLLAAQRGGITTVLIPEENQKDLVEIPDNIKGKLEIKPVRWIDEVLQVALTRQP
                     TPRPEAPGEDAAAPEKPARRGRRNNKQVQAH"
BASE COUNT         2563 a         4886 c         4548 g         2739 t
ORIGIN      
        1 gcgcccaatc gcgatcgcca gcgatacgcc gatcgaggac cagagagaat agcgaccgcc
       61 gacccagtcc cacatggcga agcggtagtc gggatgcacg ccgaattcat ccatcgcctt
      121 ggcgttgacc gacacggcgg cgaagtgttg tggcaggccc ttggccccaa gccgctgcag
      181 aatccagtcg cgcgccacct tggcgttggt ctgtgtttcc agcgtggtaa aggtcttcga
      241 gcagatcatg aacagcgtgc gctcgggatc ggcgctggcg atcagatcgg ccagctgaca
      301 gccatcgaca ttcgatacga atgcgacgcg cggtgcgccc gaggtgaatt gccgcagcgc
      361 ctcgaccgcc atggcgggtc ccaagtcaga accgccgatg ccgatgttca ccaccagatt
      421 gaagctctga tcggtgctgg aacgcacgat cccctgccgc acgtcttcgg caaagccgag
      481 catgcgatcg cgctcattgc gcaccagcgt gctgacatcc tcggaaccca ccatcagcgc
      541 agcggtactc gagcgtcgca gtgcggtgtg cagcacggcg cgattctcgg tgctgttgat
      601 ggcatcgccg cggaacatcg cctcgatacg cgagcgcaat ccaagctgat cggcgagcgt
      661 caccaggcag ttcaccacac tggcgtcaac gcgctggcgg gaatagtcgt acaccaggcc
      721 cgcggcctcg gccgagcaat gctgaaagcg ctgcgcgtcg cgcgcaaaca gctcgcgcgt
      781 cggggcggaa gcgaatgtcg gcgccaggga ttcgagttcg tgccaggcgg cgcaatcggt
      841 gccggagcgc aggggattgg ccatttcgcg gtactccacg gacctgatgg gtccggcgat
      901 tctatcggtg cgcgcccgaa tcaggtagct gcggacaacg gattcggcgg cgccagggcg
      961 agccagatgc atttgccgcc actggccttt tgcagcagcc gggtcagttc gccgtgcgcg
     1021 gcgcactcct gcgcactcgc ccggatcaca cgcagcggct cggcgggacg cacactgatg
     1081 gcgctgagcg ccgcgtgcgc acccaacccg gcctccggac ctgactcatc gagcgccagc
     1141 agattctgac cgctggtcat cgccagatag acgtcggcca gaatgcgcgc atcgagcagc
     1201 gcaccgtgca actcgcggtg cgaattgtcg atctcgtagc gcctgcacaa cgcatccaga
     1261 ttgttgcgct ggccgggatg gcgcgctcgc gccagcgtca gcgtatccag tacccggcac
     1321 accgatcgca gcgaggccgg cagcgtcgcg ctgcccagcc gcgataactc catgtcgagg
     1381 aattccacat cgaactcggc gttgtgaatg atcaattcgc tatcggcgac aaaggccagc
     1441 agctcatcgg cgatcgcggc gaagcgtggc gcgctttcga gccgctcgcg ggtgatgccg
     1501 tgcacgccga ccgcgccgta atcgatctcg cgatccggat tcaggtagcg gtgaaagatt
     1561 ctgccggtgg cgcgccggtt gatgagttcg acgcagccga cctcgatgat gcgatggccg
     1621 agtttggact cgagacccgt cgtttccgtg tcgagtacga cctgtcgcat gagatctcgt
     1681 gtcagctggg ctggcgcgag tctaccgcaa gcaacgcatc gatcgcggca tttgcgagcg
     1741 catcgcaacg ctcgttgccg ggaatgccgg agtgtcccgg cacccaatgc cactcgatgc
     1801 ggtgcttggc cacttcgccg tcgagcacct gccacagatc ctgattcttg accggcttct
     1861 tgtctgcggt tttccagccg cgcgctttcc actgcggcag ccactccagc acgccgcggc
     1921 gcacgtattg tgaatcggtg tacaggcgca cctcgaccgc acgcttgagc gcctgcagtg
     1981 cgctgatcac cgcctgcagc tccattcgat tgttggtggt cagggtctcg gcgccgctga
     2041 tctccttctc tcgcccgccg gcggaaagca ctgcggccca gccgccgacg cccggattgc
     2101 cgcggcaggc gccgtcggta tagatctcca cgacctgact catcggctcg gctctgccag
     2161 gcccccgaag acgcgcactc gctcgcgcag ccgcgggcga atgggcgtca tcgtataaac
     2221 gcgcttgcgt gccttgatca gataggccgc ggccggccag ggatacagcc agccgcgccg
     2281 caggatcccc gggatggccg cctcgacgct gcctcgaagc gggcgcggcg tgcgaaacag
     2341 gtagggatgc acgctcagga tttcgtagct cagcagcccc agccagtccc gcacacgcga
     2401 ggccgacagc gtacgacgaa gtccgggcgg aaaacccaac tgcgaggcgg ccgcccgcag
     2461 cccccagaaa cccattggcc gaaagccgag cacgatcagc tgcccctcgg ctccgagcac
     2521 tcggtccgcc tcgcgcagca cggcgtaggg atcggccgcc agctccagcg tgtgcgggag
     2581 cagcaccgcg tcgaccgtgg cattgctgat cggcaggtga gccaggctgg ccacgatgtc
     2641 accacgggcc gtcccctgag agacatcggc cacgacgctc tgacgccgga tgcgggccgg
     2701 agcgagcagc tcccgtccga caccccaggc ccccacctgc aacagctcca ggccgaacac
     2761 atcatcaaag gtggtggaca gcaactgcgc ctcggcctgg atcaacgccc ggccgaggct
     2821 ttcggtctgc cattgctgca atacgggtag cggatcagcc atgtcccact atgctcaaga
     2881 tgtgaccggt tagtctagaa acactagcaa ttttgtgaac ttacctgata cattcggctc
     2941 gcctgcccgt agacttctgg gcattaccgt aacagatcca caaaaacccg ctgcggggtg
     3001 cagaagttga gattacagcc gatctggaag gcgtccatcg cggcgctggt tttagctttg
     3061 tccgcgtgcg cccaccaacc gccgcgcgca ccgcaatcca ccgcccagct cgacatccct
     3121 ttccagcccg ccagcgcagc gccggcaacg gacctgcaac ccccggcagc agcccccagc
     3181 gctcaggtcg agcaggccga ttccgccccc gcgcttgagg ccagcgtctc gcagagcgtc
     3241 agcgaagtgc cagccgccgc cggcccggtc gattcggaag gcctgccagg cccaccgccc
     3301 gacagtgtcg tcgtggcgcg cgagccggat ctgtttgccc gactgcgcag tggcttccag
     3361 ctcagcgacg tgcaggaacc tgccgtttcg agccagctga actggttcgc caaccatccg
     3421 gacttcctcg agcgcacctg gggccgtgcc tcgctgtggc tgtactacat cgtcggacag
     3481 atcgaacagc gcaagatgcc cgcggagctg gcgctgctgc cggtgatcga aagcgcattc
     3541 gagccctacg cctattcgcg tgcccgcgct gcgggtctgt ggcagttcat ttccgatacc
     3601 ggccatcgct tcgggctcaa gcaggactat tggtacgacg gccggcgcga tccgatcgag
     3661 gccacgcgcg ccgcgctcga ctacctgcag gaactgcacg atgaattcaa cggcgactgg
     3721 ctgttggcgg tcgcggccta caactgcggc gagttggcgg tagagcgtgc catcgagcgc
     3781 aaccagcgcc tgggcaagcc gaccgatttc tggcacctca aactgccgac cgagacgcgc
     3841 ggctatgtgc cggaactgct cgccatgcgg cgcctggtgt cgaaccccga gcgctttggt
     3901 ctggagttca gcccgatccc gaacgagccg tacttcatca cgatccagac cggcggtcag
     3961 atcgacctga aggtcgtggc gcaaatggcc ggtgtcacgg ccgatgacct gtacgagctc
     4021 aatccagcct ttcatcgctg ggccaccgat ccgaccggac cgtatcacct gctggtaccg
     4081 gcggattcgg ccgaggggct gcagcaggcg gtgctacagc tcacgcccga gcagcgcatg
     4141 agcgtcgagc actacacggt tcggcacggc gacacgatcg cctcaatcgc cacgcatttc
     4201 cgcacccggc cggaagtcat ccgcgaactg aacgacctgg acgcgaacga gttaccggtg
     4261 atcgatacct cactgcgggt gccctcaccg gatattcagc taccggaaaa ggccgcgcgt
     4321 gccgcgctgc tggccgatgc gcctccggga cgtttgcgcc ggcatggcgg caagccccgc
     4381 atccacgtcg tacgtcgtgg cgacacgctc tatgcgctgg cacagcggct aggaaccgat
     4441 ccacagacgc tggcgcagat caacggcctg aatccgggtg atcggctgcg cgccggtcag
     4501 aagctgcgta tgagttccag caacgccctt gtcgccagcg cgccgtccag gacctcgacc
     4561 aagactgcca gcagcggcag cgctgcggac ggcgcgcgac gcatgaccta cgtggtgcgc
     4621 cgcggcgaca cgctgtatag catcgcccgt ctgctgcagg tgacggtcag cgatctggct
     4681 gattggaatg gcgtgtcggg caatcacagc ctgaagccgg gccagatact gatcgccttc
     4741 gtcaagtcgc gaacctaagc gtcagagaca acgaccatgg ggattctgtc gggcaaacgc
     4801 gccctgatcg tcggggttgc gaccgagcgt tcgattgcgt ggggcattgc acaggcgatg
     4861 catacgcacg gcgcccaact cgcgttcacc tacgccaacg atacgttcaa ggaacgtgtc
     4921 gaaccgctgg cggcttcgat cggctcgccg ctcgtgatgc cgctggacgt gcagctcgat
     4981 gagcagatcg actccgcatt cgcgctgctg cagcgcgaat ggcagcagat cgatatcctc
     5041 gtgcatgcgg tcgcgttcgc accgcgcgaa gcgctcagcg gtcgtttcac cgccaacaca
     5101 tcgcgtgagg cgtttcgcat cgctcacgat gtgtcgagtt acagtttcac cgcgctggcg
     5161 cgcggtgccg cgccgctcat gcagggacgc gccggtgcgt tgctgacgct gtcatatctg
     5221 ggcgcggagc gctcgatccc aaactataat gtgatgggtt tggccaaggc gagcctggaa
     5281 gccaatgtgc gcttcctcgc agcggacctc gggccgcaga acattcgcgt caatgccatc
     5341 tccgctggcc ccatcaagac gctcgcggca gcgggcatcg gcggtttccg caagatgctc
     5401 ggacaggtcg cgcagatcgc gccgatgcgt cgcaacgtga cactggagga cgtcggtaat
     5461 gccgccacct tcctgtgttc ggatatggcg gccggcatta ccggcgagat cctctatgtc
     5521 gacaacggct tcagcaacgt tggcatgagc ttcgacaacg aaggctgaag gcctgcctac
     5581 cctgatcgct acttgtcctt gttgttcggg cagctgtact cgctgttctt cgccgccttc
     5641 ggatattcgc gcgtccagcc cgccggcagc tcgaactgat cggccgacac cgctgcggtg
     5701 ctcacggcgc tggtctcgat cgtgaacgca atcacctgaa cgtagctgtc aggcagcggc
     5761 ggcggtgcat tggcagcgat cgcaccctgg ctgggatcgt tggcgccggc agcgttcttc
     5821 ttcgcgaaca gtccaccgag cagttttccg ccggcgttgg ctgccagacc ggacaggcct
     5881 ccaccgcccg cgcccgcgcc attgtcggaa tcggattgtt tcgacttgga gcagttgtcg
     5941 ccacccatga tgaggcggaa ggtggtgcgc agcggatagc ctttgagtga tgccgccttc
     6001 gcctgcatat ccttcaacgt gccgctgtac gcggccatga actgctgcgc gatgcccttg
     6061 atctgcgggc tattcgcgtc caggcccatc ttggtcagat aggccttctg gaaggctcgt
     6121 ttgtcggcgg tgccctcgag tggatcctgg gtcatccaga tatcgaagcc gtacaccatc
     6181 tcgcagatat cgccggtctt cttgtcggtg caggtctgcg acatcttgac gctcgatttg
     6241 cgcgcgtcgt gcccggcgat cgtcgcgtgt tcatcggtgg ttcgcatgtc caccttcggg
     6301 ggactgagat cgcaattaga ggtatcgccc ttgctcggcg cgccgcgcgg tgcttgctgc
     6361 tgctggcatt gcttcatctt gtccagcgtc tcctgcattt tctgctgcgc catcgcccgc
     6421 tcctccggcg tcgggaacgc ggtttccagg tacgtcttct tatccggacg cagctgccag
     6481 gtgagatcct tgtccagccg gatgatctcg ccgctcttcg cgttgccgca cagcaggctc
     6541 atgaagcctt cgcaatgaaa ctcactgtcg cgacgctgcc ggtcgtcggc cgtcatctcg
     6601 gtggtcgtgc catgcagctt cacgatgcct gcggcatcca gcgccatgct ctcctgcgtg
     6661 gtgatgtcag ccaacgcggg accggtcgcc agaacgagca gcacagccgt ggtgattgtt
     6721 tccttcatgg atcccccaaa aacttccttt gatgagccgg tgagtctagc ggatcagtag
     6781 gcgatggacg acagtgatag atgacgcgcg cttcggcagc ccaccggctg cgcaccgccg
     6841 gggcgccgca caggctaccg caatgctgat ggatactcac cgcgcatcga agccgtggcg
     6901 cgtcggccgc ggggttgccc ttgcggcggc ggtcctgctc attgcgctgg tgtggttcat
     6961 ggcgctgttc gactggaatc acctgcgagg agcgatctcg ggcctggcaa gcgcgcacct
     7021 gcaccgccag gtccggatgg atcatctgtc cgtccgggta tggcagcgca atccttccgt
     7081 cgcgatcgag catctgagcg tatccaatcc gagctgggca gcgggcaaca tggtcgaagt
     7141 cggccagctc acgttcgcga tcgagccgtg gccactgctg cgcggacgac tggtcctggc
     7201 gcgactgcag atcgatcgcc cacaggtcgt gttgctgcgc gattcgggca atcgggccaa
     7261 ctgggatttc agcgacgatc agatgaaaaa gaagcaggcg gacaagcccg ggccgccggc
     7321 gcagctaccg gtgattcacc gctttacgct caatggtggc actctcaagg tggacgacgc
     7381 gatccgtaag ctgcgtttcg acggcacgct cagcgcgaat gaaggcgcga cgcgcgcgct
     7441 gtcgcacgcc ttcgagctca agggacaggg ccagctcaat ggcaaggcgt tcgagctgac
     7501 cttcggcggc agctcactgt tcaatctgca gctcgatgag ccctacgact atgacgccga
     7561 gattcacgcc ggaccgctgc aggccacggc ccgcggcgtg atcgacaagc cgttcgacct
     7621 gggacacctc agcgcgaacc tcgatttcaa aggcgagaac ctcgcagctc tctactatct
     7681 gacgggactg gccctgccct tcaccccgcc gtttcatctg tcgggggagc tgcggcgcga
     7741 cggcatgatg taccggctgc ggaaactcca gggcaccgtc ggcgacagcg acctgcacgg
     7801 cgaactcagt gtagaggccg cgggcacgcg ccccagactg acggccgagc tcatttcgca
     7861 tgcgctggat ctggcggacc tcgccccgtc tgtcggggcc ggcgtgccga atgacaacac
     7921 cgacgcgaag tccgatacgc aagcacccag ggcccaggct gacgacaagc tgttgccgac
     7981 ctatcagttc gatttcgacc ggctgcgttc gatggacgcc tcggtcgaac tgcgcgcgga
     8041 ttctgttaag acacagaagg taccgatcac cggtgtgaac ctgaaactga atctcgagcg
     8101 cggcgtgctg accctggatc cggccgacct gacgctgccg caaggcaagc tcggcggggc
     8161 cattcgtatc aacgcgcaga acgccgttgc cgacaccagc atcgacctgc gcttgagcaa
     8221 cgtgaacctc tccgaactga agagcgccaa gatgagcgag gcgcccctgg acggggatct
     8281 gctcagccgc gtgctactgg aaggcagcgg caattccgtg catgagatct ttgccagttc
     8341 gcacggagaa atcgtggcag tgatcccgca cggcgaaatg cgccaggcct tcgccgaact
     8401 caccggcatc aatgcggcgc gggcactggg actgctgttg accggcagca ggaaggagga
     8461 cggcatccgt tgcggcattg ccgccttttc tgtggaccac ggcaaggcca ccgccgagcc
     8521 gctggtgttc gataccgaca cggtcgcagt caccggcagc ggcggatttg atttcaacac
     8581 cgaggatctg gatctgcgac tgaagggtca gtcgaagaaa ttcgagccgc tgcacgtgcg
     8641 cgcaccgatc actgtccgcg gcacgctggg gaagccgtcc atcggactgg accccaagag
     8701 cctggcggcc cagggcggcg ccgccgccgc actcggggtg ctcgcaacac cgctcgccgc
     8761 ggtggcggct ttcatcgatc cggggctggc gaagaatcag gactgctccc gactgctctc
     8821 gagcgcgccc gagaaagccg ccgagcagcc cggagcaccg caactcgcac cgaaatagac
     8881 tcgacagcgc gcgctactcg aagattttcg gattgcgctt gacgtttgcc cggcgctgca
     8941 gttccgccag atacgcgttc atcgaagcgt cgcgatcgcg ccgcagaaac tggatcacga
     9001 gctgcgtatc gttctgcgga ttggtgccgc cggcgcctgg cttcacggcg ctgacggcca
     9061 gtaccgccgc gccgccgttg tccagcgcga cggcgcggtc caccggcttg ccgttcggat
     9121 gcggcacctg gaaggcctcc tgccgaacct gcatgggaat ctgcggatca ttgcgcccga
     9181 cataggcggc cggggctgaa ctgataccca ggcccctgac gacctgatcg aacggcgtgc
     9241 cgccatccag ctgcttgacc gcattgtcgg ccgcggactt ggccgccgcc gtactctcgc
     9301 tcttcttcac ggcggcgatg atttcatcgc gcacgctcgc gatcgattgc gctgccgggg
     9361 cgcgatgctc cagcaccctg aagatcacca gatgatcgtc cgagatcgca accggaccgc
     9421 cgatcttgcc cccggcaagc atgtcatcat tgaacactgc cgccagcatc gccggactgc
     9481 ctccgagcgg cgcgccgccg gttgtccgtg tgaagtcctg aatctctccg gtcggcagat
     9541 cgaactgttt ggcgatcgca tccagatcgc ccgagccgct ctccacccgc tgctgcagct
     9601 tttcctgccg atcgccgaac agctcggcgg cctgatcgcg ccggtactcg gtttccagct
     9661 ccgctcgcac ttcgtccaag cctcgcacat gcgccggacg aatctcatcc aggcggatga
     9721 tgtgatagcc gaactgcgtc ttgacaggat cgctgatctg tccgggctgc atcgagaaca
     9781 gcgcgtccgc aaacggaccc acgaacgccg tctgatcggc ccagcccaga tccccgccct
     9841 gcgccgcaga gccggggtcg gcggaattct tcttcgccag ctctgcgaaa tccttgccgc
     9901 tcttcagatc cgcttcgatc tgattggcgc gtgcgagcgc cgccgcttcc gccttggcgt
     9961 ccttgggatc cgcaatcgag atcaggatgt ggtgcgcgtg acgcttttcg ggctcggtat
    10021 aacgggcttt gaccttctcg taataggcct gcagatcttc aggcttcaca ctcacctgcg
    10081 gcgtcagtgc gtccagccgc agctcagcgt attggagttt gaccgactcc ggggtcatgt
    10141 agtcggcgga gtgcgcgtcg taccaggctt tgattctggc atcgtcgatc ttgaggccgc
    10201 tgacgaaatg atccgccggc aacaccgcat agcgcagttc gcgctgctcg ttctccagcg
    10261 cgtagacgcg atcgagttcc gcaggggtca tgaactcgga attctcgatc gcggagttca
    10321 gctgccccac cgccatcgac tgactgcgct cgttgaggaa ggccctgtcc gtcataccga
    10381 tccgcgcgat ggcgatcgat gccgccctcg gatcgtactt gccgtccacc tggaacgccg
    10441 gctcgctgtg catgtactcg gcgatctcct gcggcgtcgc gcggtagccg tcttcttcgg
    10501 cgcgctggcg cagcacggtg ttgcgaatga actcgtccac cagcttctgc tggagcgtgc
    10561 tcttcatctc atcggtcatc tctgagccgt tgagcatctg ctggtactgc gcctgacgtt
    10621 cctgccaggc gcggttgatg gtctggttgc tgatgcgctc gccattgacc cggagcgcgt
    10681 aatcgggcgc gccgaagctc atgttgacaa cgccgtaggc accccagatg gcgaagatca
    10741 gcgccagcgg gacaagcacg agtgtggcca gccaacgctg gctcttcagt ttatcgccaa
    10801 gattctgcag catttgagta gatggcggag cggacgggac tcgaacccgc gacccccggc
    10861 gtgacaggcc ggtattctaa ccagctgaac taccgctccg tattagaact gacatcgatg
    10921 gaataaatcc gcactggctg gcttgatgcc gaacttcgaa ttatagcggc cccactgacg
    10981 ctctgccaat tggcgtgcga cgcccgtgtg cagttccccg gggctggcta ctcttcgagc
    11041 gcagtcgtac gcgatcgcga acgcacgaat tggcgccgtc cgccgcagaa gaatttcaga
    11101 aaagtctgtg cctcagggct cacaaaatcc cctcgcccca gcagtgccgc ggcgatctcg
    11161 cgcagattgc ccaccggcac gtcgagcgcg cgataggcac gtttgagttc ggccagcgcc
    11221 gctgctttca gtccgcgacg acgcagcccg accgcgttca gcccgatcag cgcactgcgc
    11281 tcggtcgcca tgcaaaatgg cggaacgtcc tgcgagatgc gcgcgccacc accgatcatc
    11341 gcgttctccc cgatgcggca gaactggtgt accaccgccc cgccgcccag aaatacccgc
    11401 tcatccacgt gcacgtgccc cgccagcagc accgcgttgg caatcacgac acgatcccca
    11461 atgcgacagt cgtgcgccac gtgacagctt gccatcagga aacagtcggc accgatctcg
    11521 gtgcaggtat cggcctgggt ggcacggctg atcgtcacgt gctcgcggat gatggtgcgc
    11581 gcaccgatgc gcacgcccga ggacacctcg ccccgaaagc tcaggtcctg aggatcaccg
    11641 ccgagcaagg cgaacgggtg cacgaccacc gcctcaccga gctcgcagtg gcgactgacg
    11701 aaggcgtagg cctggacgat acagccggtt ccgagtctcg cgccgggttc gacaatggct
    11761 gtggggtgaa tgtgccctga cggcatcaca gtatggtaga gcaatggccg cgatgagtgt
    11821 tgacagaggt atctcaatat atatagactt agtggatgcg acgaatcaga cgaccgtccg
    11881 ctcacacgct gtcgctgctg gaagccttga gccagcgtcc gcgcgcctgg cagtacggct
    11941 acgagctttc ccagatcacg cagctcaagt cagggacgct gtatccgata ctgatgcgcc
    12001 tgtccgatcg cggatttctc gagtcacgct ggcagccggc gcagcagccc ggacgcccgc
    12061 cgcgtcatgt ctaccggctc acgagctcag gtgccgcctt cgcgcgcgag gccttcgcaa
    12121 gacaggaagg cgaggcgacg cgcggtgagt tcgcatgagc gcgcaacacc cgttgtcgcg
    12181 gcaggttgca cgcgcactgt tggcgcatgc cgcccgtgtg tcgcccgcgg ggcgccgcgg
    12241 ctggttcgtg gcgatggcga gcgaactgga tcacctgccg cacggcgcat ccgtcctgca
    12301 atgggccctc ggatgcctgc tggtcgccta tacggagcgc atcatggacc gatcactcac
    12361 tcatcttccg cgctggttgc tggccaccga gatggccgtg tgcctggtgc cactgacatg
    12421 gctgttcgtc gcggtgatca tggtcaccgc acagggtcac atgacgctgg tgtggggcct
    12481 gaaatacgcc tccgcggcgc tggtgggacc gctgggactg gcgctcgcag cacgaatcct
    12541 gttcgtccgt tcaagggcgt tgggccgcgc cgcggccacg ctgctggcgc tgctggccgc
    12601 gtggaccttc attgccgagc tcggacagtc actcgacacc ggactgcccg tctcgacctg
    12661 gtggcgggat ttcgtgctga ttgcggtgct gccagcggtg gccgtgtccc atctgctgtg
    12721 gatcaatgcg gaccggcgtg cgcgcgcact acgagctcac tgatacactg atagctcact
    12781 gatacacagg gaattggtgg gtgctgaggg gctcgaaccc ccgacattcg ccgtgtaaag
    12841 gcgacgctct accagctgag ctaagcaccc aggcgcctga ggccgatgcc gatgaagcgt
    12901 agcgccatcg cgcgcgctac gcatccgcca tttcaatgac tagtgggcct gaacctgctt
    12961 gttgttgcga cggccgcgac gcgccggctt ttccggagcc gccgcatcct caccgggcgc
    13021 ctcgggacgc ggcgtcggtt gacgcgtcag cgccacctgc agcacctcgt cgatccagcg
    13081 cacgggtttg atctccagct tgcccttgat gttgtccgga atctcgacca gatccttctg
    13141 gttttcctcc ggaatcagca ccgtagtgat gccgccgcgc tgagccgcca gcagcttctc
    13201 tttcaggccg ccgatcggca gcacttcacc gcgcagcgtg atttcgccgg tcatcgccac
    13261 atccgagcgc accgggatgc gcgtcagggc cgagaccagc gccgtgcaca taccgatacc
    13321 cgcgctagga ccgtctttcg gcgtcgcacc ctcgggcatg tgaatgtgca cgtcgttctt
    13381 ctggtagaaa tcgacatcca ggccgagcac cgcggcgcgg ctgcgcacca cggacatcgc
    13441 cgcctggatc gactcctgca tgacctcgcc cagctggccg gtgtgcaata gcttgccctt
    13501 gcccggtacc accgcggctt cgatcgtcag cagttcgccg ccgacctcgg tccaggcaag
    13561 accggtcacc tggccgacgc gattcatttc ttcggccttg ccgtagcgga aacgcttcac
    13621 gcccaggtac ttgtccaggt tgcgcggcgt gactgcgacc gaaccactct tcttgtcggc
    13681 cttgtcgagc agcagctgct tcaccgcctt gcggctgatc ttggcgatct cacgctccag
    13741 attgcgaacg cccgcctcgc gcgtgtagtg acgcacgatg tccagaatcg agccgtccga
    13801 aatcttcagc tcgccatctc tcaggccgtt ctgcttggtc tgcttcggca ccaggtagcg
    13861 ccgcgcgata ttgagcttct catcctcggt gtagccgggc agccggatga cttccatgcg
    13921 atccagcagc ggtgccggaa tgttcagtga gttcgcggta cagacgaaca tcgtttccga
    13981 cagatcgaaa tccacttcca gatagtgatc ggcgaaagtc gcgttctgct ccggatcaag
    14041 cacttccagc agcgccgagg atggatcgcc acggaaatcc atggccatct tgtccacttc
    14101 gtcgagcagg aacagcggat tgtggacacc ggtcttcgac agattctgaa tgatcttgcc
    14161 gggcatcgag ccgatatagg tgcgccggtg gccacggatt tccgcttcat cgcgcacccc
    14221 gccgagcgac atgcgcacga acttgcggtt ggtggcgcgc gccatgctct gaccgagcga
    14281 ggtcttgccg acgccgggcg ggccgaccag acacaggatc gggcccttga tcttctctac
    14341 gcgctgcagc accgccagat attcgacgat gcgctccttg accttgtcca ggccatagtg
    14401 atcctcgtcc agcactttct gcgcgttgac gatgttcttg agcaggcgcg tgcgtttctt
    14461 ccacggcgcc ttgatgagcc aatcgacgta gttgcgcacc acggtcgcct cggccgacat
    14521 cggtgacatc atcttgagct tgttgatctc ggcggtggcc ttctcgcgtg cctccttcga
    14581 cataccggcc ttgaccactt tctgctcgag ttcggcaatc tcgttggtgc cgtcctccat
    14641 ctccccgagc tctttctgaa tcgccttcat ctgctcgttg aggtagtact cgcgctggct
    14701 cttttccatc tgcgccttga cgcggccgcg gatgcg
//
LOCUS       DATMRM010000312         6780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3568066, whole genome shotgun sequence.
ACCESSION   DATMRM010000312 DATMRM010000000
VERSION     DATMRM010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3568066"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VNZ06_09305"
     CDS             <1..540
                     /locus_tag="VNZ06_09305"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HWW20987.1"
                     /translation="YGMLIFVIIAVFIAGLMVGRTPEYLGKKLQAFEMKMASLVILIP
                     PFFSLVGTAIAVSFATGRDAVGNNPGPHAFSEILYAFSSASNNNGSAFAGLSANNPFY
                     NSLLGIVMWTSRFWVIVPVLAIAGSLAAKKRVPASAGTLATHSPLFVVLLAATVVIVG
                     ALAFFPALALGPIVEELQF"
     gene            537..2612
                     /gene="kdpB"
                     /locus_tag="VNZ06_09310"
     CDS             537..2612
                     /gene="kdpB"
                     /locus_tag="VNZ06_09310"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012076314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HWW20988.1"
                     /translation="MSSAARSRPLFDPPLVKGAIRESFIKLAPWFQWRNPVMFVVLVG
                     SVLISALWLVQLGGSLRNEGHPSFVLAIALWLWATLLFANFAESLAEGRGKAQAAALR
                     LGRRDVSARRYPNGHIDQPYETIASRQLRRGDIVLLEAGDTIPGDGEVVWGTASVDES
                     AITGESAPVIREAGGDRSAVTGGTRLLSDTLRVRITANPGEAFLDRMIAMVENAKRQR
                     TPNEIALAILLAGLTVIFLKTTATLLPFSLYSVGAAGHGTPISITVLVALLVCLIPTT
                     IGGLLSAIGIAGMDRMIQANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRLASRLIP
                     APGVTPEELADAAQLSSLADETPEGRSIVVLAKQQYGIRGRDINALGARFIAFSAQTR
                     MSGVDIDGRQIRKGAAEAIDRWVAEHGGALPVEVTRAVESVARQGATPLVVADGAHAL
                     GVIELKDIVKGGIRERFAELRRMGIRTVMITGDNKLTAATIAAEAGVDDFLAEATPEA
                     KLRLIREHQQQGRLVAMCGDGTNDAPALAQADVAVAMNTGTQAAKEAGNMIDLDSNPT
                     KLIEVVEVGKQMLITRGSLTTFSIANDVAKYFAIIPAAFATTYPRLDALNIMRLSPNG
                     AILSAVIFNALIIIALVPLALRGVRYRPIGANVLLRRNLLIYGLGGILVPFIGIKLID
                     LILLALHLE"
     gene            2622..3194
                     /gene="kdpC"
                     /locus_tag="VNZ06_09315"
     CDS             2622..3194
                     /gene="kdpC"
                     /locus_tag="VNZ06_09315"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HWW20989.1"
                     /translation="MAKILLQSVCVLLLLSLLTGVVYPLIVTGIAQLCCARQSGGSLI
                     VRQGHVLGSSLMAQPFTEDKYFWPRPSATTPDPYDGLASGGSNLGPTNPAQIDAVKQN
                     LQALQQSDPSNRLPVPVDLVTASGSGLDPDISPAGADYQVARVARARGLDPSRVRQLV
                     AEHTDLRQWGLLGEPRVNVLELNLALDRLR"
     gene            3213..5921
                     /locus_tag="VNZ06_09320"
     CDS             3213..5921
                     /locus_tag="VNZ06_09320"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HWW20990.1"
                     /translation="MNASRPDPDLLLTRLREAEARARRGRLTIFFGANAGVGKTYAML
                     EAAQRRRAEGIDVVAGYVELHGRGETERLLAGLEQLPPMTIRVGETSRREFDLDAALA
                     RKPCLLLVDELAHTNISGGDPPPRHEKRWQDVEELLDAGIDVYTTVNVQHLESLSDVV
                     WQITGVRQLETLPDRLFDEADEIKLVDVTPEDLLQRLREGRIYRPQQAQQALENFFRK
                     GNLIALRELALRKTADRVDQAMRDYRDEKAVRNAWAARERLLVAVGPDAQAESLVRAG
                     KRTADRLDAEWLVVYVETPDLLRLSEHERNRRISILRLATSLGAEAITLGGASASDEI
                     LAYARTRNVSRILVGAPNRQRWQRLFRSSVTERLLERSRDIDVLVIARNQSRGPAPVV
                     EAAPDSIAPAESERQRWPRYLLAIAGTVVATLVCWTSYRWYPGLGQANLVMIYLLNSA
                     VIAVYGGRRAAILSSFLGVLAFDFVFVPPRFSFAVTDAQYVITFGVMLAVALIIGNLN
                     ASVRLQARVAGYRERRTSLLYAMSRQLAMARGRDEMANVAVHHVHQVFSSRAVILFPD
                     EHGKLPAPRLPQTPESFNDADLGVAQWVFDHAKPAGLGTDTLSGAAGLYLPLLGGDRV
                     FGVLGVLPDNPRRVTLPEQYRLLETFAAQIGQAMERADFAAHAQAAEVRAETEAIRNA
                     LLASISHDLRTPLATITGGAATLAGNLEALSESDRKALATSVSEEATRMSERLTTLLD
                     LVRLETGAIQPRYDDYAIEELVGSALHRLDSRLRHHRVRTELPETLPLVHVDGRLIEQ
                     VIENLLDNAVKYTPEGTEIRIAAQAQTRQVEVSVADDGPGLPATDPDVLFEKFQRGAP
                     EGSVGGIGLGLAICRTILGLHKGRIWAENRVPHGAVFRFTVPIEPP"
     gene            5918..6616
                     /locus_tag="VNZ06_09325"
     CDS             5918..6616
                     /locus_tag="VNZ06_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW20991.1"
                     /translation="MTHSSRAVLVVEDEPQLRRVLRTLLELEQFRVIESENAERGVLE
                     ARSHKPDLLIVDLGLPDRDGMSVIQDVRRWSRVPIIVLSARTDEAAIVAALDAGADDY
                     VTKPFGARELMARLQVALRHSVSAGAVGSMLNIGAWQIDLKARTVRDDAGNELHLTPT
                     EYRLLEVLAEHIGLVVTHRALLQQVWGPGSTQQTHYLRVFMKQLRSKLEPDPGRPNWL
                     LTETGVGYRLIQRD"
BASE COUNT         1177 a         2142 c         2245 g         1216 t
ORIGIN      
        1 tacggcatgc tgatcttcgt gatcatcgcg gtgttcattg ccgggctcat ggtgggtcgt
       61 acgcccgagt acctcggcaa gaagctgcag gcgttcgaga tgaaaatggc gtcgctagtc
      121 attctgatac cgccgttctt ctcgctggtg ggtactgcga tcgcagtctc gtttgccacc
      181 ggccgcgacg ctgtgggcaa caatcccggt ccgcatgcgt tctcggaaat cctgtacgcg
      241 ttttcatccg cctccaacaa caacggcagt gcgttcgcag gcctgtcggc caacaacccc
      301 ttctacaact cgctgcttgg catcgtcatg tggacgtcgc gcttctgggt gatcgtgccg
      361 gtgctggcga ttgccggaag cctggcggca aagaagcgcg taccggcgag cgccgggacg
      421 ctggccaccc acagcccgct gttcgtggtg ctattggccg ccaccgtggt gatcgtcggc
      481 gcacttgcct tctttcccgc gttggcgctg ggacccatcg tcgaggagtt gcagttttga
      541 gctccgccgc gcgtagccgc ccgctgttcg acccaccgct ggtgaagggc gcgatccgcg
      601 aatccttcat caaactggcg ccgtggtttc aatggcgaaa cccggtgatg ttcgtcgtgc
      661 tggtgggtag cgtactgatc agcgcactat ggctggtgca gctgggcggg agcttgcgca
      721 acgagggaca cccgagtttc gtgctggcga tcgcactgtg gctgtgggcg acgctactgt
      781 tcgccaactt cgcggagagt ctggccgaag gccgtggcaa ggcgcaggcc gcggcgctgc
      841 gcttgggaag acgtgacgtg tccgcgcggc gctaccccaa tggacacatc gatcagccgt
      901 acgagacgat cgcatcgcgt cagctgcgcc gcggcgatat cgtgttgctc gaagcaggcg
      961 acaccatccc gggcgatggc gaagtcgtat ggggaactgc ttcagtggac gagagtgcga
     1021 tcaccggtga atccgcaccg gtcatccgag aagccggtgg cgaccgctcc gcagtcaccg
     1081 gtggcacgcg gctgttgtcc gacacgctgc gcgtgcgcat cactgccaat ccgggcgaag
     1141 ccttcctcga tcgcatgatc gcgatggtgg agaacgccaa gcgtcagcgc acgcccaacg
     1201 aaatcgcact ggcgattctg ttggctggcc tgacggtcat tttcctcaag acgaccgcca
     1261 cactgctgcc gttctcgctc tacagcgtcg gagcggccgg gcacggcacg ccgatttcaa
     1321 tcacggtgct ggtggcgctg ctggtgtgcc tgattccgac gacgatcggc ggcctgctgt
     1381 cggcgatcgg catcgccggc atggaccgca tgatccaggc gaatgtcatc gccacctcgg
     1441 ggcgtgccgt ggaagccgcc ggcgacgtgg acgtgctgct gctggacaag accggaacca
     1501 tcacactcgg caaccggctc gcctcgcggc tgatcccggc gcctggtgtt accccggagg
     1561 agctggcgga tgctgcacag ctctcctcgc tcgcggatga aacgccggag ggccgatcga
     1621 tcgtcgtgct ggccaagcag caatatggca tccgcggccg ggacatcaat gcgctcggcg
     1681 cgcgcttcat cgccttcagc gcgcagaccc gtatgagcgg ggtcgacatc gacggccggc
     1741 agatccgcaa aggcgccgcg gaggcgatcg accgctgggt cgccgagcat ggcggcgcac
     1801 tgccggtgga agtgacgcgc gcggtcgaaa gcgttgcgcg ccaaggcgcc acaccgctgg
     1861 tcgtcgccga cggcgcccat gcgctcggcg tcatcgaact caaggacatc gtcaagggcg
     1921 gcatccgtga gcgcttcgcc gagttgcggc ggatggggat tcgaaccgtg atgatcacgg
     1981 gcgacaacaa gctcaccgcc gccactatcg ccgcggaggc gggcgtcgat gattttctgg
     2041 ccgaggctac gcccgaggcc aagctgcggt tgatccgcga gcatcagcag caaggacgcc
     2101 tggtggcgat gtgcggcgat ggcaccaacg atgcgccggc gctggcacag gcggatgtcg
     2161 cggtggcgat gaataccggc acgcaggcag ccaaggaagc cggcaacatg atcgatctgg
     2221 actccaaccc caccaagctg atcgaagtgg tggaggttgg caaacagatg ctcatcaccc
     2281 gcggttcact cacgaccttc agtattgcga acgacgttgc gaagtatttc gcaatcattc
     2341 ctgctgcctt cgccaccact tacccacggc tcgatgcgct gaacatcatg cgtctgagcc
     2401 ccaacggggc cattctgtcg gcggtgatct tcaacgcgct gattatcatc gcgctcgtgc
     2461 cgctggcgct gcgcggcgtg cgctaccgtc ccattggtgc gaacgtgttg ctgcggcgca
     2521 acctcctgat ctatgggctg ggcggcatcc tcgtaccgtt catcggcatc aagttgatcg
     2581 acctgatcct gcttgccctg cacctggagt aggaacgcat cgtggccaag atcctgttgc
     2641 aatccgtgtg cgtgctgctg ctgctgagct tactgaccgg cgttgtctat ccgctcatcg
     2701 tcactggcat cgcgcagctg tgctgtgcgc ggcagtcggg cggatcgctg atcgtgcgac
     2761 agggccacgt gctcggctcg agcctcatgg cccagccctt taccgaggac aagtacttct
     2821 ggccacggcc atccgccacg actcccgatc cctatgacgg tctggcgtcg ggtggttcga
     2881 atctggggcc gaccaacccg gcgcagatcg acgcggtaaa gcagaatctg caggctctgc
     2941 agcagtccga tccgtccaat cggctacccg tacctgtaga tctggtgacc gcttcgggca
     3001 gcgggctgga tccagacatc agtccggccg gggccgacta ccaggtcgcg cgcgtcgccc
     3061 gcgcccgggg actggatccc tcccgcgtgc gccagctggt ggccgaacac acggacttgc
     3121 gtcagtgggg tcttttgggt gagcctcggg tcaatgttct ggagctcaat ctcgcgctcg
     3181 accggctgcg ctgacgcact gactataaat cgatgaacgc aagccggccg gacccggacc
     3241 tgctgctcac gcgtctgcgt gaagccgagg cacgtgcgcg ccgcggtcga ctcaccattt
     3301 tctttggcgc caatgcgggc gttggcaaga cctacgcgat gctggaggcg gcgcagcgtc
     3361 gccgcgcaga gggcatcgat gtggttgccg gctacgtcga actgcatgga cgcggcgaga
     3421 ccgaacggct cctggccggc ctggaacagc ttccaccgat gactattcga gtgggcgaaa
     3481 ccagccggcg tgaatttgat ctggacgcgg cactggcgcg caaaccctgt ttgctgctgg
     3541 tggacgagct ggcacacacc aacatcagcg gcggtgatcc ccctccccgg catgaaaaac
     3601 gctggcagga cgtcgaagaa ctgctcgacg cgggcatcga tgtctatacc acggtgaacg
     3661 tgcaacacct cgagtccctg agcgacgtcg tgtggcagat cacgggcgta cgacagttgg
     3721 agacgctgcc cgatcggctg ttcgatgaag ccgatgagat caagctcgtg gacgtcacgc
     3781 ccgaggatct gctgcagcgg ctgcgcgaag gacgcatcta tcgaccgcag caggcgcaac
     3841 aggcactgga gaattttttt cgcaagggca atctgatcgc gttgcgcgag ctggcattgc
     3901 gcaagaccgc ggatcgcgtg gaccaggcga tgcgcgatta ccgcgacgag aaagcggtac
     3961 gcaacgcctg ggcagcgcgc gagcgattgc tggtcgcagt cggtccggat gcacaggctg
     4021 aaagcctggt gcgcgccggc aaacgtacgg ccgaccggct cgatgccgag tggctagtgg
     4081 tgtacgtgga aacgccggac ctattgcgtc tgtccgaaca cgagcgcaat cgccggattt
     4141 cgatcctgcg gctggccacc tcgctcggag ccgaagcgat tacgctcggc ggtgcctcgg
     4201 ccagcgatga aatactcgcc tacgctcgca cccgcaatgt ctcgcgcatc ctggtagggg
     4261 ctccgaatcg ccagcgttgg cagcgcctgt ttcgctcctc ggtgaccgag cggctgctgg
     4321 agcgcagtcg cgacatcgac gtgctggtga tcgcgcgcaa tcaaagccgc gggccagcgc
     4381 cggtcgtcga ggcagcgccc gactcgatcg cacctgccga gtcggagagg cagcgctggc
     4441 cacgctatct gctggccatc gcaggcactg tcgtcgccac gctggtgtgc tggacttcct
     4501 atcgctggta ccctgggctg ggacaggcga atctggtgat gatctatctg ttgaactccg
     4561 ccgtcatcgc ggtctacggc ggacggcgcg ccgcgatcct gagttcgttc ctgggcgtac
     4621 tcgcgtttga cttcgtattc gtgccgccgc gcttcagctt tgccgtgacc gatgcgcagt
     4681 atgtgatcac gttcggcgtg atgctcgcag tcgcgctgat catcggcaac ctgaatgcca
     4741 gcgtacggct gcaggcgcgt gtcgccgggt atcgtgagcg tcgcacctcg ttgctgtacg
     4801 cgatgtcgcg ccagttggcg atggcgcgcg gacgcgacga gatggcgaac gtcgccgtcc
     4861 accacgtgca ccaggtattc agcagtcgtg cggtgatcct gttcccggat gagcacggca
     4921 aactgccggc accgcgactg ccgcagacac cggaatcatt caacgacgcg gatctcggag
     4981 tggcgcagtg ggtgttcgat cacgccaaac cggccggact cggcaccgat acgctcagcg
     5041 gtgcggccgg gctgtatcta cccctgctcg gcggcgaccg tgtgttcggc gtactgggcg
     5101 tgttaccgga taacccacgg cgcgtcacgc tgcccgagca ataccggctg ctggagacct
     5161 ttgccgcgca gatcggtcag gcaatggaac gggcagactt cgcggcgcac gcccaggcgg
     5221 cagaagtgcg agccgaaacc gaggcgatcc gcaacgcact gttggcctcg atctcgcacg
     5281 atctgcgcac accgctggca acgattaccg gcggggcagc gaccctggcc gggaacctgg
     5341 aggcgctgag cgagtcggat cgcaaagcgc tggccacctc agtgagcgaa gaagccacgc
     5401 gcatgagtga gcgactgacc acgctgctcg atcttgtgcg tctggagacc ggcgccattc
     5461 agccccgcta cgacgactac gcgatcgagg aactggtggg ttcggcgctg catcgactgg
     5521 attcacggct gcgccatcat cgcgtgcgca ccgagctgcc tgagacgctg cccctggtgc
     5581 atgtggacgg ccggctgatc gaacaggtga tcgagaatct gctcgacaac gccgtcaagt
     5641 acacgcccga aggcaccgag atacgcatcg cggcgcaggc tcagacccgc caggtggagg
     5701 tgagcgtggc ggatgatggt ccgggactgc ccgcgaccga cccggacgtc ctgtttgaaa
     5761 agttccagcg cggcgcgccg gagggctccg tcggcggcat cggactgggg ctggcgatct
     5821 gccgcacgat tctcgggctg cacaaggggc gcatctgggc cgagaaccgc gtgccgcacg
     5881 gcgccgtgtt ccgtttcacc gtaccgatcg agccgccgtg acccactcca gcagggccgt
     5941 gctcgttgtc gaggatgagc cgcagttgcg acgcgtactg cgaacgttgc tggagctgga
     6001 acagtttcgc gtgatcgagt ccgagaacgc cgagcgtggc gtgctcgagg cgcgcagcca
     6061 caaacccgat ctgctgattg ttgatctggg tctgcccgac cgcgatggga tgagtgtcat
     6121 ccaggatgtg cgccgctggt cacgcgtacc gatcatcgtg ctgtcggcgc gcaccgatga
     6181 ggccgcgatc gtcgccgcgc tggatgccgg cgccgacgac tatgtcacca aaccgtttgg
     6241 cgctcgtgag ctgatggcac gactgcaggt ggcgcttcga cattcggtga gcgcgggcgc
     6301 cgtcggctcc atgctgaaca tcggcgcgtg gcagattgac ctgaaagcac gcacggtgcg
     6361 cgatgatgct ggcaacgaat tgcacctgac gcccaccgaa taccggctgc tcgaagtact
     6421 ggccgaacac atcgggctgg tggtcactca tcgcgccctg ctgcagcaag tgtggggacc
     6481 gggcagtacg cagcaaacgc actatctgcg cgtgttcatg aaacagctgc ggtcgaagct
     6541 ggaaccggac ccggggcggc cgaactggct gctgacggaa accggggtcg gctaccggct
     6601 gattcagcgc gactgacgtg aagcgcgcgg cggcgcctca ggccgcgatc ccgtattgcc
     6661 gcagtagcgc cgcgacctcc ggtttgcggc cgcggaactc aacgaaagcg tccagtgcgt
     6721 cgcggctgcc accacgcgag agaatgctgt cgagaaagcg ccgcgcggtg gtccgatcga
//
LOCUS       DATMRM010000313         5047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1750926, whole genome shotgun sequence.
ACCESSION   DATMRM010000313 DATMRM010000000
VERSION     DATMRM010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750926"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VNZ06_09330"
     CDS             complement(<1..811)
                     /locus_tag="VNZ06_09330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW20992.1"
                     /translation="MLNPADDGAAARRTILFCVLAALCEGIDLQAAGVAAGGIRAEFH
                     PTDDQLGNFFSASTFGLFFGALLGGWLADRIGRKWVLVCSIALFGLFSLLTPLAWDIQ
                     SLAYARLLTGLGLGGALPNILALVSESSRHDRRHANVALAYSGNPFGGAFISLVTLLL
                     AATRWRAIFVVGGVLPLILAPIMAFTLQESAAFRAAAHTPSPQISRGNLLAVLREGRA
                     IGTLLLWISFFLGLLTLYLLLNWLPILLQGDGFSRSQSAGAQIGFNIGGAIA"
     gene            complement(824..2662)
                     /locus_tag="VNZ06_09335"
     CDS             complement(824..2662)
                     /locus_tag="VNZ06_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HWW20993.1"
                     /translation="MTPDRLAWRPGSFAVSVHTDTDGSILLTPTTALAGYPLRVTDEL
                     ERWAALAPERIFVAQRDHQAAWRRVSYAQALQQVRRLANALIALNLSPDRPLAILSGN
                     SIEHLLLSLAALYIGVPYCPISPAYSQAAPDFTKLRYVMQLLTPGLVAAFNSARFERA
                     ILSAVAPGVPLLGDCDAIGERKLHPWQRFASDEAPLAQAAHQRVGADTIAKFLLTSGS
                     TGHPKAVITTHRMLCSNQIMLRSALPFLADEPPVLVDWLPWNHTFGGSHNVGAVLFNG
                     GTLYIDEGKPTAADFEATVRNLSEISPSVYFNVPKGFELLAQRMERDARLRESFYRRL
                     RACFFAGAGLTQYTWDALDRQALAARGQRLPILSGLGATETAPSVTFTTPDTDRAGVI
                     GLPAPGCVVKIAPVGDKRELRVRSPGVTPGYWRAPEQTRAAFDSQGFYRLGDAVRLID
                     PGDPTRGLVFDGRIAEDFKLSSGTWVSVGPLRAGLLQALAPLAVDVVIAGLNRDYLAV
                     LIVPDAPACSQELGAAGELSPPELARDARLLAKIAARLTMHARAHTGGATRVRRAMIV
                     PEAPSLDRGEITEKGSINQRLMLARWEDLLERLYGGEPAAHVMTID"
     gene            complement(2665..3537)
                     /locus_tag="VNZ06_09340"
     CDS             complement(2665..3537)
                     /locus_tag="VNZ06_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016883831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWW20994.1"
                     /translation="MKIDELIAIDVHTHAEVSCRQEPDEAWKPYEEAAGKYFKAGKRP
                     TIAETIAYYRERKIGLVMFTVDSEFQVGNRRIPNEEVADAARDNADMMIAFASIDPHK
                     GKMGVREARRLIEDGVIRGFKFHPTLQGFFPNDRMAYPLYELIAEHKLPALFHSGHSG
                     IGTGMPGGGGLRLKFSNPIHLDDVAADFPDMTIIIAHPSWPWQDEALSVCMHKPNVYI
                     DLSGWSPKYFPPQLVQYANSQLKHKMLFGSDFPLIAPDRWMRDFKDAGFKPDVHELIL
                     KSNAVRALQLSNGA"
     gene            complement(3534..>5047)
                     /locus_tag="VNZ06_09345"
     CDS             complement(3534..>5047)
                     /locus_tag="VNZ06_09345"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HWW20995.1"
                     /translation="AERSVDPASASDEVFDRMSARAQRDYPNRQAGLTAVAAVRAATG
                     MPFVAGLLEEERLVNLAKATPESRGAVHLFFAERQTRHVEGLPPSTPLAVKCAGIIGA
                     GTMGGGIAMCFANAGIPVRLLDTDSVALARGLSVVSANYDSMVKRGRIDADEKARRLG
                     LIQGSLDYADLAQADVVIEAVFESMDLKRKIFAALDQAARPGAILASNTSTLDVTQIA
                     RATSRPGDVIGMHFFSPANVMPLLEVVRTDLTSARTIHSVMELSRALRKTPVLARVCY
                     GFIGNRMMEGYAREAERMLLEGATPRQIDSALERWGMAMGILAVFDMAGVDVGVNVHT
                     ANAERFPPDPTYYQADFALSEAGRLGQKTGKGYYRYVPGDRARHDDPEAIEILRARAQ
                     KLGVTQHSHTEQEILERCLYPLLNEGFRILEEGVAQRASDIDVVWAAGYGFPRYRGGP
                     MFYAETIGLPTLLQGMQHYAKRCGPMHWQPASLLVQLVEQRRSIADWERERAS"
BASE COUNT          957 a         1715 c         1576 g          799 t
ORIGIN      
        1 ctgcgatcgc accaccgata ttgaagccga tctgcgcgcc agcggactgc gaacgcgaga
       61 acccatcacc ctgcagcaaa atcggcagcc agttgagcag cagatacagg gtcagcagtc
      121 cgaggaagaa actgatccac agcagcagcg tcccgatcgc acggccctcg cgcagcacgg
      181 ccagcagatt gccgcgggag atctgcggac tgggggtgtg cgcggcagcg cggaacgcgg
      241 cggactcctg caaggtgaac gccatgatcg gtgccagaat gagcggcagc acgccaccca
      301 ccacgaagat cgcgcgccag cgggtcgcgg cgagcaacag tgtcaccaga ctgatgaacg
      361 cgccaccgaa cggattgccg ctgtaggcca gagcgacgtt ggcgtgacgc cggtcatggc
      421 gactgctctc cgacaccagc gcaagaatgt tgggcagcgc ccctcccaga ccgagcccgg
      481 tcagcaggcg cgcataggcc agcgactgaa tgtcccacgc cagcggcgtg agcaatgaga
      541 acaggccgaa cagcgcgatc gagcacacca gcacccactt gcgcccgatg cgatccgcga
      601 gccagccacc cagcagtgca ccgaagaaca gaccaaacgt actggcactg aagaaattcc
      661 ccagctgatc gtctgtcggg tgaaactcgg cgcggatacc gccggccgcc acgcccgcag
      721 cctgcagatc gattccctcg cacaacgccg ccagcacaca aaaaagtatg gtgcgccgcg
      781 cggcggcgcc gtcatcggcc ggattgagca tgcagatccc ccttcagtcg atggtcatca
      841 cgtgcgcggc aggctccccg ccatagagtc gttccaacag atcctcccaa cgcgccagca
      901 tcaaccgctg attgatcgag cccttctccg taatctcacc ccgatccagc gacggcgcct
      961 caggcacgat catcgcgcgt cgcacgcgcg tggcaccgcc ggtgtgcgcg cgcgcatgca
     1021 ttgtcagccg ggcagcgatc ttcgccagca gacgtgcatc ccgcgccaac tccggcggcg
     1081 agagctcgcc cgcggcgccc agctcctgcg agcaggccgg cgcatccggc acgatgagca
     1141 cggccagata gtcgcggttg agaccggcga tcacgacatc gacggccagc ggcgccaagg
     1201 cctgcaacag cccggcgcgc aacggaccca cgctcaccca ggtgcctgaa ctcaacttga
     1261 aatcctcggc gatccggccg tcaaacacca gtccgcgcgt cggatcaccg ggatcgatca
     1321 gtcgcaccgc gtccccgaga cgatagaaac cttgcgagtc gaacgccgcg cgcgtctgct
     1381 cgggtgcgcg ccagtagccg ggcgtgacac cgggcgagcg cacccgcagc tcgcgcttgt
     1441 ctcccaccgg cgcgatcttg accacgcagc ccggcgccgg cagtccgatc acgccagcgc
     1501 ggtcggtatc cggagtggta aaggtcactg acggtgcggt ttcggtggcc ccgagtcctg
     1561 acagaatcgg caggcgttgg ccgcgcgccg ccagcgcctg acggtcgagt gcatcccacg
     1621 tgtactgggt cagccccgcg ccggcaaaga aacacgcgcg cagccggcga tagaagctct
     1681 cacgcagccg tgcatcgcgc tccatgcgct gcgccagcag ttcgaaaccc ttgggtacat
     1741 tgaagtagac gctcggagag atctccgaca ggttgcgcac cgtcgcctca aaatcagccg
     1801 ccgtcggctt accttcatcg atgtagagcg tgccgccgtt gaacagcacc gcgccgacgt
     1861 tgtgactgcc gccgaaggtg tgattccacg gcagccagtc caccagcacc ggcggttcat
     1921 cggccagaaa cggcaatgcc gagcgcagca tgatctggtt gctgcagagc atgcgatggg
     1981 tcgtgatcac cgctttgggg tggccggtcg aaccggaggt cagcaggaat ttggcgatcg
     2041 tgtcggcgcc gacgcgctgg tgtgccgcct gcgcgagagg cgcctcgtcc gaagcaaagc
     2101 gctgccaggg atggagtttg cgctcgccga ttgcatcgca gtcgcccaac agcggcacac
     2161 cgggcgccac cgcactgaga atcgcgcgct cgaagcgagc gctgttgaag gccgcgacca
     2221 gccccggggt cagcagctgc atcacgtagc gcagcttggt gaaatccgga gcagcctgag
     2281 aataggcggg cgatatcgga caatagggca cgccgatata cagggcggcg agactgagca
     2341 gcagatgctc gatgctgttg cccgagagga tcgcgagcgg ccggtcgggc gagagattca
     2401 atgcgatgag ggcgttggcg agccggcgca cctgctgcag cgcctgcgcg tagctgacgc
     2461 ggcgccaggc cgcctgatga tcgcgttgcg cgacgaaaat acgttccgga gccagggcgg
     2521 cccagcgttc gagttcatcg gtgacgcgca gcggataccc ggcgagcgcc gtagtcggtg
     2581 tcagaaggat cgaaccgtca gtatcggtgt gcacgctgac ggcaaacgag cccgggcgcc
     2641 acgccaggcg atcaggcgtc atcgctacgc cccgtttgag agctgcagcg cgcgcaccgc
     2701 gttgctcttc agtatcagct cgtgcacgtc gggcttgaag ccggcgtcct tgaaatccct
     2761 catccagcga tccggagcga tcagcgggaa gtccgagccg aacagcatct tgtgcttgag
     2821 ctgactgttg gcgtactgca ccagctgcgg cggaaaatat tttggcgacc agcccgacag
     2881 atcgatgtag acgttcggct tgtgcatgca caccgacaac gcctcgtcct gccatggcca
     2941 ggagggatgc gcaatgatga tcgtcatgtc cggaaagtcg gccgcgacat catccaggtg
     3001 aatcggattg gagaacttca ggcgcaggcc accgccgcca ggcatcccgg tgccgatacc
     3061 cgagtgcccg ctgtgaaaca gcgccggcag tttgtgctcg gcgatcaact cgtacagcgg
     3121 ataggccatc cgatcgttcg gaaagaatcc ctgcagcgtg gggtgaaact tgaatccgcg
     3181 gatgacgccg tcctcgatca ggcgtcgggc ctcgcgcacc cccatcttgc ccttgtgcgg
     3241 atcaatgctc gcgaaagcga tcatcatgtc ggcgttgtcg cgtgcggcat cggcgacctc
     3301 ttcgttcgga atgcgccggt tcccgacctg aaactcggag tccacggtga acatcaccag
     3361 cccgatcttg cgctcacgat agtaggcaat cgtctcggcg atggtcggtc gcttgccggc
     3421 cttgaagtac ttgcccgccg cctcctcata gggcttccag gcctcgtcgg gctcctgccg
     3481 gcacgagacc tcagcgtgag tgtggacatc gatcgcaatc agctcatcga ttttcatgac
     3541 gctcgctccc gttcccagtc agcgatgctg cgacgctgtt ccaccagttg caccagcaac
     3601 gaggccggct gccagtgcat cgggccgcag cgcttggcgt aatgctgcat gccctggagc
     3661 agggtcggca acccgatcgt ctccgcatag aacatgggcc cgccgcgata gcgcggaaat
     3721 ccgtaaccgg cggcccacac cacgtcgatg tcgctggcgc gctgtgccac accctcttcc
     3781 aggatgcgaa acccttcgtt cagcagcggg tagagacagc gctccaggat ttcctgctcc
     3841 gtgtgcgagt gctgcgtcac gcccagcttc tgcgcgcgcg cccgcagaat ctcaatcgcc
     3901 tccgggtcat catggcgggc gcgatcaccg gggacatagc ggtagtagcc cttgccggtt
     3961 ttctggccca gacgtccggc ctcggacagc gcgaaatcgg cctgatagta cgtcggatcc
     4021 ggcggaaagc gctccgcgtt cgccgtgtgc acattgacgc caacatccac acccgccata
     4081 tcaaacacgg ccaggattcc catcgccatg ccccagcgct ccagggcact gtcgatctgt
     4141 cgtggcgtcg ccccctccag cagcatgcgc tcggcctcgc gcgcatagcc ttccatcatg
     4201 cggttgccga tgaacccgta gcacacgcgc gccagtaccg gcgtcttgcg cagcgccctg
     4261 gacaactcca tgaccgagtg gatggtgcgc gccgaggtca gatccgtgcg caccacctcc
     4321 agcagcggca tcacattggc cggtgaaaag aaatgcatgc cgatgacatc gcccgggcgc
     4381 gaagtcgccc gcgcaatctg cgtgacatcc agcgtcgagg tgttgctggc cagtatcgcg
     4441 cccggtctgg cggcctgatc gagcgccgcg aagatcttgc gcttgagatc catgctctca
     4501 aacaccgcct cgatcacgac gtcggcctgc gcaaggtcgg cgtagtcgag cgaaccctgg
     4561 atgagcccca ggcgccgggc cttctcgtcg gcgtcgatcc tgccgcgctt gaccatggag
     4621 tcatagttgg ccgacaccac cgacaggccg cgcgctagcg caacgctgtc ggtatcgagc
     4681 agcctcaccg gtatgccggc attggcaaag cacatcgcga tgccaccccc catggtgccc
     4741 gcgccgatga tgcccgcgca cttcaccgcc agcggcgtgg atggcggcaa cccctcgacg
     4801 tgacgcgtct gtcgctcggc aaaaaacaga tgcaccgcgc cgcggctttc cggtgtcgcc
     4861 ttggcaagat tgaccaggcg ctcttcctcg agcaacccgg cgacaaacgg catgccggtt
     4921 gcggcgcgca ccgccgccac cgccgtcaga cccgcctggc ggttgggata gtcgcgctgc
     4981 gcccgcgcgc tcatgcgatc gaacacctca tccgaggcgc tggccggatc gacgctgcgc
     5041 tcggcgg
//
LOCUS       DATMRM010000314        10479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4211046, whole genome shotgun sequence.
ACCESSION   DATMRM010000314 DATMRM010000000
VERSION     DATMRM010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10479
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4211046"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3530
                     /locus_tag="VNZ06_09350"
     CDS             <1..3530
                     /locus_tag="VNZ06_09350"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWW20996.1"
                     /translation="QLAQLATGHRTTLIFANTRRMVERVARHLSERIGDENIAAHHGS
                     LAKEQRLSAEQRLKSGDLRVLVATASLELGIDIGEVELVCQLGTPRSISTFLQRVGRA
                     NHAVDGVPKGRLLPLSRDDLIECVALLQAVRSGELDALRIPPQPLDVLAQQLVAEISA
                     REYGEDELYELVRRADPYRELSRQNFEAVVQMLSEGISTRRGRQSAYLHRDSVNRRLR
                     ARRGARLTAITCGGAIPDNADYQVVLEPSATFIGTLNEDFAIESHAGDIFQLGNCSYR
                     ILRVEAGRVRVEDAKGQPPNLPFWLGEAPARSTELSCAVSALRAEVNAALPIVDASHI
                     ARAVALIAQRHQLPQVAARQLVEYLAAAKAVLGDLPTRHRVVFERFFDEAGDMHLVIH
                     SPYGARLNRAFGLALRKRFCRSFNFELQAAATEDAIVLSLGETHSFPLEEVAHYLHSN
                     TVEALLIQAVLDAPLFTTRWRWNATISLAVKRARAGKKTPAPLQRMAAEDLMAVVFPD
                     QLACAENMTGEREIPNHPLVQQTLYDSLHDAMDIHGLRTLLRAMERGEVVTVTRDLPA
                     PSPLAQEILTARPYAFLDDAPLEERRTNAVANRGWLDPETATRFGQLDPAAIDAVRSQ
                     SWPSCENADELHDALMLVGVMDSARAGADGLQPLFAQLLQQRRACEWQVDARRFWIAV
                     ENLPMLRAVYAQGRAVPELAVPAVYAARHWDSGEALRELLRGRMQAVGPTTAIDLAAS
                     LTLPAAAIDAALYALESEGFAMRGQFSPQAAASEWCERRLLARIHRYTLQSLRAQIEP
                     VSTADFMRFLLDWQGLTVQPRPEGPASLERALEQLEGFEIAAAAWEEEVLPARLQQYD
                     PSWLDTLCLSGRVYWARVSRGSGGGTGPVRATPIALLTRQHRSLWAQVASIERDDARL
                     SAYARAMADYLQCHGASFFDEMLQGTGLLQAHAESALAELVAAGLASADSFGGLRALL
                     MPLQRKRQLAARGRRRVLSGVEEAGRWSLLKRGSSVARSSDALEDVESVAWLLLHRYG
                     VVFRRLLTRESEWLPPWHQLLRAYRRLEAQGHVRGGRFVGGVSGEQYALPDAVGALRS
                     VRKRAPEGELIALSAADPLNLAGIVTPGARLAALAGNRLLLRDGITVATFAAGEVQFH
                     IELPPHEQWQTRHALLRKGARPARLARMG"
     gene            3558..4256
                     /locus_tag="VNZ06_09355"
     CDS             3558..4256
                     /locus_tag="VNZ06_09355"
                     /inference="COORDINATES: protein motif:HMM:NF025221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HWW20997.1"
                     /translation="MQAASPRTMIVAGLLIGLLSGPALALSLSDITNQEASGGIKGAL
                     NSGIDTAVGKLGVPGGFLNNSKVKIPLPPALEQVASGMRMLGQGKRADELVAKMNEAA
                     EQAVPLAKPLLVSAVKSMSLADAKQILTGGDDSVTKFFRAKTSAPLAVKFLPIVKHST
                     DRVGLAQQYDEFAGEGAKLGLVKGDAGSIEKYVTDKALDGLYLMIGEEERAIRRDPAA
                     AGSALVEKVFAALH"
     gene            4323..5009
                     /locus_tag="VNZ06_09360"
     CDS             4323..5009
                     /locus_tag="VNZ06_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWW20998.1"
                     /translation="MSLQFERGTVNAVIGTSGSGKSTLLRLLLGLEWPDSGSITIDGA
                     AMSREGRLALRQRIGYVIQEGGLFPHLRVRDNLALLPRHLRWSREHIDARARELLELM
                     QLPADALERFPGELSGGQRQRVAVMRALMIDPPALLMDEPLGALDPLVRFELQQRLRE
                     LFKVLAKTVIVVTHDLAEAAYLAPRLIMMQAGSVVQDGSTSDFVQRPANEFVRGFVAA
                     YRSGERALDA"
     gene            5006..6502
                     /locus_tag="VNZ06_09365"
     CDS             5006..6502
                     /locus_tag="VNZ06_09365"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWW20999.1"
                     /translation="MSKRGIGLACLALSCATALAASPPEVRIGSKNFTESVILSEMLR
                     LDAQRHGMTLEHRRAMGGSAILWQALRQGSIDAYPEYTGTLTQELLRGLPPSASHQQL
                     SQALLPLGLDLSESIGFEDRYALGVPEALGERLQLRSLSDLRAHPELRLALSNEFMNR
                     ADGWPGLRAAYALQPASVRGVEHSLAYRALASDAADVIDLYTTDAEIQSYHIRVLEDD
                     RHYFPGYLAVWLYRLDSARRAPRLLRAIADLTSRISDTQMQQLNARVQVQRRDETSVA
                     SEFLSSGAASGAASAGTWQRIRTRTVEHLELVSVSLGLAMLLGLPLGVAAAKLPRLGK
                     LVLGLAVVLQTVPSLAMLVFMIPLFGIGARPAIATLFLYSLLPIVRNTLTGLTSIPAR
                     LQESAASLGLPWQVRLLRVEAPLAARTIIAGISTAAVINVGTATLGALIGAGGYGEPI
                     LTGIRLNDLPLILEGAIPAAALALLVMAGFQLLERALTPRGLRLSFDG"
     gene            complement(6568..6873)
                     /locus_tag="VNZ06_09370"
     CDS             complement(6568..6873)
                     /locus_tag="VNZ06_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HWW21000.1"
                     /translation="MTTRKRWLASWLIVTASLAPAVSMSGVVIDIDVAPPAPQVEEVP
                     APRSGFVWAPGYWDWRGHNHVWVQGHWIQGRQGYHWVPDHWIQRGPHWHHEVGRWEH"
     gene            7218..7343
                     /locus_tag="VNZ06_09375"
     CDS             7218..7343
                     /locus_tag="VNZ06_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21001.1"
                     /translation="MTTRIILQLLLLPVVMIGVPLMFAVAWLHELIDDRRRSLAA"
     gene            7389..8540
                     /gene="dinB"
                     /locus_tag="VNZ06_09380"
     CDS             7389..8540
                     /gene="dinB"
                     /locus_tag="VNZ06_09380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HWW21002.1"
                     /translation="MTQAHTSTHIRRIAHLDMDAFYASVELLRYPHLRGLPLVIGGGR
                     AGEPRELRHYVGRGVVTTATYAAREFGVHSAMGLMKAAQLCPQALLLPADFEEYRKFS
                     RLFKTVIAEFTPFIEDRGIDEVYIDLTEVEGAQEQGGRTLALRLQQAIHERTGLTCSI
                     GVAPNKLLAKMASEFNKPNGISIVYESELADKVWPLPCRKIHGIGPRMSERLEQLGIR
                     TVGELARCEREWLVEHFGPNSGTWMHAAAWGWDDRPVVTHCEPVSMSRETTFERDLHA
                     VKDRSELSELFTSLCEHVAQDLQRHGYVGKTIGIKLRFDNFQRITRDQTIVDYTNDAA
                     TIRRTAGLCLKRAPLERRLRLLGVKVASLRRSDGRCAGKVETQGDLSLF"
     gene            complement(8550..9389)
                     /locus_tag="VNZ06_09385"
     CDS             complement(8550..9389)
                     /locus_tag="VNZ06_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HWW21003.1"
                     /translation="MGHRFLPIILVLSMLAMGTSPAAGATTPLDLARSVRTQGCRGHA
                     GIRTPLRYIGGLNEAALSVAQGAPLKTAVARSGYREQESTSIHISGDASALQQVLSNQ
                     LCDTLLNPDFGDVGIAQRGHDTWMIFAVPFTPPPATSAGPVGTELLQRINQARAKARR
                     CGSKLFAAAPALQPNLLLRAAAEAHARDMLEHNYFAHEGHDGSNPAQRVAAEGYGYRL
                     IGENIASGPTTAAEAVAGWLASPEHCENLMDARFTDSGIGFSASTHGLPRIYWVQEFG
                     TPR"
     gene            complement(9404..9805)
                     /locus_tag="VNZ06_09390"
     CDS             complement(9404..9805)
                     /locus_tag="VNZ06_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045735 - nutrient reservoir activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWW21004.1"
                     /translation="MTLAVPRIKIRKLKVHADERGSVFEPLEPTLLSGWQNVHTVITE
                     PGGVRGNHRHLRGTEVTSVLGPALVRYRNGDEVEEMNVPAGEAWRFLFPPGTAHAFKN
                     TGTRPLLLVSFNTELHDAAHPDAEREELIAP"
     gene            complement(9848..10423)
                     /locus_tag="VNZ06_09395"
     CDS             complement(9848..10423)
                     /locus_tag="VNZ06_09395"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWW21005.1"
                     /translation="MSSFYQGVIGTSWAVWVLYWIVSAANVKRTLRREPLHSRAAHFL
                     PMVLAFGLLAAPPGPDTEFLFARFVPVMTLLQDFATLMVVSGLLFAVWARVHLGSNWS
                     GRVTLKENHELIRTGPYQLVRHPIYTGLLLAILGTALALGEWRGLLATVMMVISFWRK
                     LQLEEALMRQTFGDEYRRYCEHTAALIPYVI"
BASE COUNT         1827 a         3237 c         3519 g         1896 t
ORIGIN      
        1 accagctggc gcagctcgcc accgggcacc gcacgacgct gatctttgcc aacacccgcc
       61 gcatggtcga gcgcgtggcg cgccacctga gcgagcgcat cggcgacgag aatatcgccg
      121 cgcatcacgg tagcctcgcc aaagagcaac gcctgtcggc ggagcaacgc ctgaagagcg
      181 gtgacctgcg ggtgctggtg gccaccgcgt cgctcgaact cgggatcgac atcggagagg
      241 tcgagctggt gtgccagctc ggtacgccgc gctcgatttc cacattcctg caacgggtgg
      301 gtcgcgccaa tcacgcggtc gatggtgtgc ccaagggtcg gctgctgccg ctgagccgcg
      361 acgatctgat cgagtgcgtg gcgttgctgc aggcggtccg cagcggtgaa ctcgatgcgc
      421 tgcgcatccc gccgcagccg ctggatgtgc tggcacaaca gctggtagcg gagatcagtg
      481 cgcgcgagta cggcgaggac gagctgtatg agctggtgcg acgcgctgac ccgtatcgcg
      541 agctgtcgcg ccagaacttc gaggccgtcg tgcaaatgct gtccgaaggc atcagcacgc
      601 gtcgcggccg ccagagcgcc tatctgcacc gcgacagcgt caaccggcga ctgcgtgccc
      661 gacgcggtgc gcgcctgacc gccatcacct gcggtggcgc gattccggac aatgcggact
      721 atcaggtcgt gctcgagccg agcgccactt tcatcggcac gctcaatgag gacttcgcga
      781 tcgagagtca cgccggcgac atctttcagc tcggcaactg ctcgtatcga attctgcggg
      841 tcgaggccgg tcgcgtgcgg gtggaggacg ccaagggcca accgccgaac ctgccgttct
      901 ggttgggcga agcacccgcc cgcagcactg aactgtcctg cgcggtctcg gcgctgcgcg
      961 ccgaggtcaa tgcggcgctg ccgattgtgg acgccagcca tatagcgcgc gcggtcgcac
     1021 tcatcgcgca gcgccatcaa ctaccgcaag tcgcggcgcg ccagctagtc gaataccttg
     1081 cggcagccaa agcggtactc ggcgatctgc ccacgcgaca cagggttgtg ttcgagcgat
     1141 ttttcgatga ggccggcgat atgcacctgg tgattcattc gccctacggt gcgcgcctga
     1201 atcgtgcatt cggcctggcc ctgcgcaagc gcttttgtcg ctcgttcaat ttcgaattac
     1261 aggcggcagc caccgaggac gcgatcgtac tgtcgctcgg tgagacgcac agctttccgc
     1321 tggaggaggt ggctcactat ctgcacagca atacggttga ggcattgctg atccaggccg
     1381 tgctcgatgc accgttgttc accacgcgct ggcgctggaa tgcaaccatt tcgctggcgg
     1441 tgaagcgtgc gcgtgctggc aagaagacac cggcaccgct acagcggatg gcggccgagg
     1501 atctgatggc ggtcgtgttc cccgatcagc tggcctgcgc cgaaaatatg acgggcgagc
     1561 gcgagatccc gaatcatccg ctggtgcagc agacgctgta cgacagcttg cacgatgcga
     1621 tggatattca cggcctgcgc acgctgctgc gagcgatgga gcgcggtgag gtcgtgaccg
     1681 tgacgcgtga cctgcctgcc ccgtccccct tggcacagga gatcctgact gcgcggccgt
     1741 atgcgttcct cgacgatgca ccgctggaag agcgtcgtac caatgccgtg gcgaaccgcg
     1801 gctggctcga tcccgaaacg gcgacccggt ttggacagct cgatccggcc gcgattgacg
     1861 cggtgcgctc tcagtcctgg cccagctgcg agaacgcgga tgaactgcac gatgcgctga
     1921 tgctggtcgg tgtgatggac agcgctcgcg ccggggccga cggcctgcaa ccgctgttcg
     1981 cgcagctgct gcagcagcga cgagcctgtg aatggcaagt cgatgcccgg cgcttctgga
     2041 tcgcagtgga gaatctgccc atgctgcgcg ccgtctatgc tcaaggacgc gccgtgcccg
     2101 agctggcggt gccggccgtc tacgccgcac gtcactggga cagcggcgag gcgctgcgcg
     2161 aactgctgcg cggtcgaatg caggcggtcg ggccgacgac cgcgatcgat ctggcggcat
     2221 cgctgacgct gccggctgcc gcgatcgatg cggcgttgta cgcgctggag agcgaaggtt
     2281 ttgcgatgcg cgggcagttc tcgccgcaag ccgcagcgtc ggaatggtgc gagcggcgcc
     2341 tgctggcgcg tattcatcgc tacaccctgc agtcgctgcg cgcgcagatc gaaccggtgt
     2401 caaccgccga tttcatgcgc ttcctgctcg actggcaggg cctcacggtg cagccgcgac
     2461 cggaagggcc ggccagcctg gagcgcgcac tcgagcagct tgaaggattt gaaatcgcgg
     2521 cggccgcgtg ggaggaagag gtcctgcccg cgcgcctgca gcagtacgac ccgagctggc
     2581 tcgacactct gtgcctgtcc ggacgtgtgt actgggcgcg agtgagccgt ggtagcggtg
     2641 gcggcacagg tccggtgcgc gcgactccga tcgcgttgct gactcgccag catcgctcgc
     2701 tctgggcgca ggtggcgagc atcgagcgcg acgatgcgcg gttgtcagcg tatgcacgcg
     2761 cgatggcgga ctatctgcaa tgccatggcg cctcgttctt cgatgagatg ctgcaaggta
     2821 ccgggctgtt gcaggcgcac gctgagagtg cgctggcgga gctggtggcg gcaggtcttg
     2881 cgagcgccga cagttttggc ggtttgcgag ccttactgat gcccctgcag cgcaagcgac
     2941 agcttgccgc gcgcggtcgg cgccgggtgc tgtcgggcgt cgaggaggcc ggccgctgga
     3001 gtttgctgaa gcgcggttcg agcgttgcgc gcagcagcga tgcgctggag gatgtcgagt
     3061 cggtcgcctg gctgctgctg catcgctacg gggtggtgtt ccggcggctg ctgacgcggg
     3121 agtccgagtg gttgccgccc tggcatcagt tgctgcgtgc ctatcggcgg ctcgaagcgc
     3181 agggacatgt tcgcgggggc cgtttcgttg gcggcgtcag cggcgagcaa tacgcactgc
     3241 cggatgccgt gggtgcactg cgatcggttc gcaaacgcgc gccggagggt gagttgatcg
     3301 cactcagcgc cgccgatccc ctcaatctgg cgggcatcgt gacacccggc gcgcggctcg
     3361 ccgcgctggc gggcaaccga ctgctgctgc gcgatggcat caccgttgcc acctttgccg
     3421 caggagaggt gcagtttcat atagagttgc caccgcacga gcaatggcag acacgtcacg
     3481 cgttgctgcg aaagggtgcg cgtccagcgc ggttggctcg catgggctga gacgtcagca
     3541 ttcgaaggag gtcgagcatg caagctgcat cgccgcggac gatgatcgtg gcgggtcttc
     3601 tgatcggcct tttgtcggga ccggccctcg cgctgagtct gtcggacatc accaatcagg
     3661 aagcctccgg cggcatcaaa ggtgcgctca acagcgggat cgacaccgcg gtcggcaaac
     3721 tgggggtgcc gggcggattt ctgaacaact ccaaggtgaa gatcccgttg ccgcccgcgc
     3781 tcgaacaggt cgccagtggc atgcgcatgc tgggacaggg caaacgggcc gatgaactcg
     3841 tcgccaagat gaacgaggct gcggagcagg ccgtgccgct ggcgaagccg ctgctcgtca
     3901 gcgcggtcaa atcgatgagt ctggctgacg cgaagcagat cctgaccgga ggcgatgatt
     3961 cggtaacgaa attctttcgt gccaagacct cggccccgct agctgtgaaa tttctgccga
     4021 tcgtcaaaca cagcaccgac cgcgtcggtc tggcgcagca atacgatgag ttcgcagggg
     4081 aaggcgcgaa actcggcctg gtcaaaggcg atgcgggcag cattgagaaa tacgtgactg
     4141 acaaggcgct cgacgggctc tacctgatga tcggggaaga ggagcgcgcc attcggcgcg
     4201 atccggcggc cgccggcagt gcgctggttg aaaaagtatt cgcagccttg cactaggggt
     4261 tcatcctgtt caggctggag cacgtcagta aacgattcgg cacgatagcg gcactggaag
     4321 acctgagcct gcagttcgag cgcggcaccg tgaatgcggt catcggtacc agcggctcgg
     4381 gcaagtccac tctgctgcgg ctgttgctcg gcctggagtg gccggacagc ggctcgatca
     4441 ccatcgacgg cgcggcgatg tcgcgcgaag gacgcctggc gctgcggcag cggataggct
     4501 acgtgattca ggagggcggc ttgttcccgc atctacgcgt gcgagacaac cttgcgctgc
     4561 tgccgcggca tctgcgctgg tcacgcgagc atatcgatgc gcgcgcgcgc gagctgctgg
     4621 agttgatgca gctgccggcg gatgcgctgg agcgctttcc gggagagctt tcgggcggac
     4681 agcggcaacg cgtggcggtg atgcgggcgc tgatgatcga tcctccggcg ctgctgatgg
     4741 atgagccact cggagcactc gatccgctgg tgcggttcga actgcagcag cgtctgcgtg
     4801 agctgttcaa ggtactcgcc aagacggtga ttgtagtgac tcacgatctg gccgaggccg
     4861 cgtatctggc accgcgactg atcatgatgc aggctggaag cgtggtgcag gatggcagca
     4921 ccagcgattt cgtgcagcgt ccagccaatg agttcgtgcg cggtttcgtg gccgcatatc
     4981 gatccggcga gcgcgcactc gacgcatgag caagcggggg atagggctcg cctgcctggc
     5041 gctgagctgt gcgaccgcgc tcgcggcatc gccgcccgag gtgcgaattg gctccaagaa
     5101 tttcaccgag tcggtcatct tgagcgagat gctgcggctg gatgcgcagc gccacgggat
     5161 gacgctcgag caccgccgtg cgatgggcgg cagtgcgatc ctttggcagg cgctgcgaca
     5221 gggaagtatc gacgcctatc ctgagtacac cgggaccctc acacaggaac tgctgcgcgg
     5281 tctgccgccg tcggccagcc accagcagct gtcgcaagcg ttgctgccgt tgggactcga
     5341 tttgagcgaa tccataggct tcgaagatcg ctacgcactg ggcgtgcccg aggcgctcgg
     5401 cgagcgtctg cagttgcgat cgctgtcaga tctgcgtgca caccccgaac tgcgcttggc
     5461 gttgtcgaat gagttcatga accgcgcgga cggctggcct ggactgcgcg ccgcttatgc
     5521 gttgcaaccc gccagcgtcc gaggagtcga gcacagtctc gcctatcggg cgctggcgag
     5581 tgatgccgcc gatgtcatcg atctctacac cacggatgcc gagatacaga gctatcacat
     5641 ccgggtgctc gaagatgacc ggcactactt ccccggctac ctggccgtct ggctttatcg
     5701 tctggacagt gcgcggcgcg cgccgcggtt gctgagggcg atcgcggatc tgacgtcgcg
     5761 catttccgac acgcagatgc agcagctgaa cgcacgggta caggtgcagc ggcgcgatga
     5821 gacctcggtg gcttcagagt tcctgtcctc gggagcggcc agcggtgctg cgagtgcggg
     5881 tacctggcag cgcattcgca cgcgcaccgt cgagcatctg gagctggtca gtgtctcgct
     5941 gggactggcg atgctgctcg ggctgccgct cggcgtcgcc gcggccaagc tgccgcgcct
     6001 ggggaagctg gtactcggcc ttgcggtagt gctgcaaacg gtgccatcgc tcgcgatgct
     6061 ggtgttcatg atcccgctat tcgggatcgg cgcccggcct gcgatcgcga cactgttcct
     6121 ctacagtctg ttgccgatcg tgcgcaatac gctcaccggc ctcacatcga ttccggcccg
     6181 tctgcaggag tccgccgcat cgctcggtct accctggcag gtgcggctgc tgcgcgtcga
     6241 ggcgccgttg gccgcgcgca ccatcattgc cgggatcagt accgcggcag tcatcaacgt
     6301 cgggaccgca acgctgggcg cactgatcgg cgcgggaggc tatggcgagc cgatcctcac
     6361 aggcatccgg ctcaacgatc tgccactgat tctggagggt gccattcccg ctgcagccct
     6421 ggcgctgctg gtgatggcgg ggtttcagct gctcgagcgc gcgctgaccc cacgcggctt
     6481 gcgcctgtca ttcgacggct gagacaaacc gcaccacagg cactcgcgct cccgcggccg
     6541 gtctggcatt cgacggctcg atagggctta gtgttcccag cgaccgactt cgtggtgcca
     6601 atgcggacca cgctgtatcc aatggtcggg tacccagtga tagccctgtc gtccctggat
     6661 ccaatggccc tgaacccaca catggttgtg tccgcgccaa tcccagtagc cgggtgccca
     6721 gacaaaccct gagcggggag cgggcacttc ctcgacctgc ggcgcaggag gcgcgacgtc
     6781 gatatcgatc accactccac tcatgctcac ggctggcgcg agcgaggcgg tgacgatcag
     6841 ccacgatgca agccatcgtt tgcgcgtagt cattctgcag tctccttctg acgcgtagat
     6901 cctaccgatc cctgaccgct aaatgctccc aagctctctc gcgggagtcg actccagagg
     6961 tcccacaagc cccgtccggc gctgtccggt tcagtgttcg cggacacctt gtcgagcgag
     7021 gtcgagcgag cgatgtgcca tgagcgatgt gccatcgata ctaataaaaa caatagatta
     7081 ccagaaataa cgagttggca cggactttgg cgcacatcac tgatcctgcc gttgcctgcc
     7141 tgaaacgcgc cgcggtagca cttcagcttc ggttcagggg atgcgctcca gactgctacg
     7201 ccaagaggac ctgaaaaatg accacccgaa tcatccttca gctattgctc ctgccggtcg
     7261 tgatgatcgg tgtgcccctg atgttcgcag tggcctggct gcacgagctc atcgacgatc
     7321 gcagacgcag tctggccgcc tagccacgaa tttcgacgag gtcggatcgg tccgcctatc
     7381 atggcggtgt gacgcaggca cacaccagca ctcacattcg acgcatcgcg catctggata
     7441 tggatgcgtt ctacgcctcg gtcgagctac tgcgctatcc gcatctacgc ggacttccgc
     7501 tcgtgatcgg cggtggtcgg gcgggcgagc cgcgagaact gcgccactat gtcggtcgtg
     7561 gagtggtcac caccgcaacc tatgcggccc gcgagttcgg cgtgcattcc gccatggggc
     7621 tcatgaaagc ggcgcagctg tgtccgcagg cgctgttgct gccagcggac tttgaggaat
     7681 accgcaaatt ctcacggctg ttcaaaacgg tgatcgccga gttcacgcca ttcatcgagg
     7741 atcggggcat cgatgaggtc tatattgatc tgaccgaggt cgaaggcgcc caggaacagg
     7801 gtggcaggac actcgcactg cgactgcagc aggcgatcca tgaacgcacg gggcttacct
     7861 gttcgatcgg tgtcgcgccc aacaagctgc tggccaagat ggcgagcgag ttcaacaagc
     7921 cgaacggcat ctcgatcgtc tacgagtcgg aactcgcgga caaggtctgg ccgttaccct
     7981 gccgcaagat ccatggtatc gggccacgga tgagcgagcg actcgagcag ctgggcatcc
     8041 gcacggtcgg cgagctggcg cgttgcgagc gcgaatggct ggtcgaacac ttcggaccca
     8101 actccggcac gtggatgcac gcggccgcgt ggggttggga tgatcgtccg gtggtcacac
     8161 actgcgagcc ggtgtcgatg agccgcgaga ccaccttcga acgcgatctg cacgcggtca
     8221 aagatcgatc ggagctgagc gaactgttca cctcactgtg cgagcacgtg gcgcaggacc
     8281 tgcagcgtca cggctacgtc ggcaagacca tcggcatcaa gctgcgattc gacaacttcc
     8341 agcgcatcac gcgcgatcag accatcgtcg actacaccaa cgatgcggct acgattcgcc
     8401 gtaccgccgg cctgtgtctg aagcgcgcgc cgctggaaag gcgcctgcga ttgctggggg
     8461 tcaaggtcgc aagcctgcga cgcagcgacg ggcgctgcgc cgggaaagtg gagacacagg
     8521 gcgacctgtc gctgttctag tagcccgcct cagcgaggcg tgccgaattc ctgaacccag
     8581 tagatgcggg gtagtccgtg ggtgctggcc gaaaacccga ttccggagtc ggtgaagcgc
     8641 gcgtccatca gattctcgca gtgctccggg cttgcgagcc aacccgcgac cgcttcggct
     8701 gcggtggtcg ggccggacgc gatgttctcg ccgatcaggc ggtagccgta cccttcggcc
     8761 gcgacgcgct gtgccggatt gctgccgtcg tgtccttcgt gcgcgaaata gttgtgctcc
     8821 agcatgtcgc gcgcgtgcgc ttcggccgca gcgcgcagca acaggttcgg ttgtagcgct
     8881 ggcgcggccg caaacagttt gctaccgcaa cggcgtgctt tggcgcgtgc ctggttgatc
     8941 cgctgcagca gttcggtgcc gaccgggccg gcgctcgtgg ccggcggtgg cgtgaacggg
     9001 acggcgaaga tcatccaggt atcgtgtcca cgctgggcaa tgccgacgtc gccgaaatcg
     9061 ggattgagca gtgtgtcgca cagctgattc gatagtacct gctgcagcgc ggaggcgtcg
     9121 ccgctgatat gaatcgaggt cgattcctgc tcgcggtagc cggagcgcgc gaccgcggtt
     9181 ttcagcggcg cgccctgagc gaccgacagc gccgcttcat tgagtccgcc gatgtagcgc
     9241 agcggcgtac gaatgccggc gtgaccgcga cagccttgcg tgcgcacgct gcgcgcgaga
     9301 tccaatggag tagtcgctcc ggccgcaggc gaggtaccca ttgccagcat cgacagcacc
     9361 agaatgatcg gcaggaagcg gtgtcccacg caactagaac cggtcaggga gcgatcagtt
     9421 cttcgcgctc ggcgtcggga tgggcggcgt cgtgcagctc ggtattgaag gaaaccagca
     9481 gcagcggtcg tgtgcccgtg ttcttgaaag catgcgctgt acccggcgga aacaggaagc
     9541 gccacgcctc accggccggg acattcattt cctcgacttc atcgccattg cgatagcgca
     9601 ccagcgccgg gccgagtacg gatgtcacct cggttccacg aaggtgtcga tgattgccac
     9661 gaactccccc cggctcggtg atcaccgtgt gcacgttctg ccagccactc agcagcgtgg
     9721 gttccagggg ctcaaacacg ctgccgcgct cgtcggcatg taccttgagc ttgcgaatct
     9781 tgattcgggg gacggcaagg gtcatttggc tctcatcata ggacggtgcg ttcctctgcg
     9841 gtgtgcatca gatcacatac ggtatcagcg cggcggtgtg ctcgcagtag cgacgatatt
     9901 cgtcgccgaa ggtctggcgc atcagcgcct cctccagttg caacttgcgc cagaaggata
     9961 tgaccatcat tacggtggcc agcaggccac gccactcgcc gagcgcgagc gcggtgccga
    10021 gaatcgccag caacaggccg gtatagatcg ggtgacgcac gagctggtaa gggccggtgc
    10081 ggatcagctc atgattctct ttgagcgtta cccggccgct ccagttcgag ccgagatgta
    10141 cccgggccca gacggcgaac agcagcccgc tcacgaccat caacgtggcg aagtcttgca
    10201 gcagcgtcat cacgggtacg aaccgggcga acagaaactc ggtgtccgga cctggcggtg
    10261 ccgccagcag tccaaaggcg agcaccatcg gcaggaagtg ggcggcgcgg gagtggagcg
    10321 gttcgcggcg tagcgtgcgt ttgacattgg cggccgagac tatccagtag agtacccaaa
    10381 cggcccatga ggtccctatc acgccttggt aaaaactact cacggccggg ccagtgtaat
    10441 aaatccgacg tctctacgtg tttgaatccg atagtcgcg
//
LOCUS       DATMRM010000315         7110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3572777, whole genome shotgun sequence.
ACCESSION   DATMRM010000315 DATMRM010000000
VERSION     DATMRM010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3572777"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VNZ06_09400"
     CDS             complement(<1..659)
                     /locus_tag="VNZ06_09400"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HWW21006.1"
                     /translation="MAKAKFTFAQFKELYPDDDACLLAILKRRYPTIDACPNCGVVGP
                     LTRIYDVLADGTRKGRRAFACKEGCHIYPCAGTVFEHSSTTLTKWFHAMYLMTATRNG
                     VAAKELERQLGVTYKCAWRIGHQLRQLMAARDKANTPEQLGGHTEIDEAYVGGHRRMK
                     YLGESRLDNKTAVVGMVERGGALKAQVVPDTKKDTLVPLVLQNVAPGTTVSTDEAHSY
                     K"
     gene            751..1665
                     /locus_tag="VNZ06_09405"
     CDS             751..1665
                     /locus_tag="VNZ06_09405"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWW21007.1"
                     /translation="MWLASEMAAFAHVAELKSFTAAARVLGVPKVAVSRAIVSLERRL
                     GTRLLTRTTRRVALTNAGLLLRPHCQRLVAEVDAVRMRFAPATDSVRSLRLIVDPGYG
                     RLLVAPLVPRFLERYGDFQLQVALVNSMPEEANKDWDLLICNAAPERPDLICTALGNP
                     SLLLCATPLYLARQGRPRAPTDLSLHSVLRVVATTEAEGGASLRLRRDCEEQQIRLKP
                     ALQVNDPALVHSSTVASLGIGVLPEFMCRQGLAMGKLERVLPDWSLAAPLRLQAVYPT
                     ELAGSAAIRLFIEFLLANMAPVLGGDAG"
     gene            complement(1662..3449)
                     /locus_tag="VNZ06_09410"
     CDS             complement(1662..3449)
                     /locus_tag="VNZ06_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWW21008.1"
                     /translation="MYQAPLRDLRFVLDDLLGVARLAELPRYRDFSIELTGAVLEEAA
                     NFAQDVLAPINRTGDRVGASWREGAVQMPTEFRNAYRRFVEGGWTQLSAPTEFGGQGA
                     PLVLSSAVEEIDFGANMAFTACPLLSRSAAEAIETVASKQLQSLLLPRIVSGEWTGTM
                     NLTEPQAGSDLAAIRTRATPDADHFRITGQKIFITYGEHDLTPNIVHLVLARIDGAPS
                     GIKGISLFAVPKFLIRADGTLGARNDVRCVSIEHKLGIHASPTCVMAYGDAGGAFGYL
                     VGQPHRGLEYMFIMMNAARLSVGIQGVGLSELARQQAAAWAHSRIQGRPIGIRVEQPV
                     PIVYHPDVRRMMLTLRANVEAMRALMLSAALQLDLARAHPDEAQRATALTRGELLIPI
                     VKGWCTEQATQMASLAIQVHGGMGFIEETGVAQTLRDARIAAIYEGTTAIQANDLLGR
                     KLGRDRGAAMAALLRDTLRELNDLRGTDATLRSVRNSGIEAITVLRDATEVLLYQMGE
                     DAGRAYAVSVPYLMLCGRVLGGALLARSAALANARLSDGTADPDFYRAKLQTARFYAE
                     HLLPESLSLLRVIKSGGACVSDAEPQLLV"
     gene            3610..4953
                     /locus_tag="VNZ06_09415"
     CDS             3610..4953
                     /locus_tag="VNZ06_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWW21009.1"
                     /translation="MMGFLDTPPSAKNGANATASGSAPAESRERRFNTDDISKQLLRI
                     LQPLHVDSLSLHDGAGELLWLNEGAFGPDEHGVVLDALDVFALDADREDLQLKLDDDR
                     TALCACARSGGGEVLGIALTIVETPASEEFAAKLAAPRLSGLMRRLAQLLAPSSPAAP
                     HATGPAAAASAVPAASKKRAEAISFSDSEDITEELEADPRPRQVVVPRERRTQPAPSA
                     KVAARSGTIHARRYARLRAGGAARRYEVKLAPNASFAADFTLATRVVEHLQRSGDRYT
                     QTPSSFAIPLSTQSVTQAGWLSKLQPILARANLPDHLIGFCLPPAAWDQELEATRRFI
                     GECEEQRCFVALDDFTLGSYGLTLLQSTAVKCLKLDGILISSVVNDKFAHATLAAIVQ
                     AARVLGLYCVAKQVSSASDVKWLAAAGIEFADGVSRGLAVAATMKSDDLSVHLGE"
     gene            5554..6372
                     /locus_tag="VNZ06_09420"
     CDS             5554..6372
                     /locus_tag="VNZ06_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21010.1"
                     /translation="MLAASPNPPDQDELPPLMPPPAPAAPPPSAAAAPAPAPGRIDRE
                     FVVKNRLVERYIGGRLPVRGAQDLERFCREHPDLLDEIALTDHINAALRLLDASGRAS
                     PWEPEPKKWWEQLWVPIAAATLALILGVTSLVLSGKLGGRDHTIAQLKQQVAQQPLEP
                     TTSTRSITVMPSRVAPSRHSMATLGGAASAEMADLKIDVSWTPLDQFRVTMDRIDQGR
                     VAIFHNLKRDSNGVLHLELNSSALGPGDYQLTIEGLDRWGNPIPQAWATISIVH"
     gene            complement(6385..>7110)
                     /locus_tag="VNZ06_09425"
     CDS             complement(6385..>7110)
                     /locus_tag="VNZ06_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWW21011.1"
                     /translation="QWWSAADRGKPWVSLSMEYRDSLGLKLGDRLRFDVAGESFDVTV
                     SSFRDVKWDSFRPNFFVVFAPGVLEGTAGTYMTSAFLQPSGGAMAQLVHRFPGVSIFN
                     VGDLLAQVRAVIDKAVTAVQSVFLFTLLAGLTLLLAAVQASREERLYETAVLRVLGAR
                     RSMIFTSMLVEFALIGALAGLLAACGASVGGAWLAHSLDLNYRFNAWLWSIGVFGALI
                     VVATAGTLATRSVVRVPPRAVLY"
BASE COUNT         1307 a         2280 c         2204 g         1319 t
ORIGIN      
        1 tctttgtagc tatgcgcttc gtcagtgctc actgtggtgc ccggagccac gttctgaagt
       61 acaagcggca ccagcgtgtc tttcttcgtg tcaggcacta cctgagcctt cagcgcgccg
      121 ccgcgctcga ccatgccgac taccgctgtt ttgttatcca agcggctttc acctaggtat
      181 ttcatacgac gatggccgcc aacgtaggct tcatcgatct cggtatggcc gccaagctgc
      241 tccggggtat tcgccttgtc cctagcagcc atgagttgcc gcagctggtg cccgatacgc
      301 catgcgcact tgtaggtgac tccaagctgg cgctctagct ctttggccgc gacaccattg
      361 cgggtagcgg tcatcaggta catggcgtgg aaccatttcg tcagagtcgt gctggaatgc
      421 tcaaagacgg tcccggcaca cggatagata tggcagccct ccttgcaggc gaacgcgcga
      481 cggcccttgc gagtgccatc ggcgagaacg tcataaatgc gcgtgagagg gccgaccacg
      541 ccgcaattcg ggcaggcgtc aatcgtcgga tagcgcctct tgaggatcgc tagcaggcag
      601 gcgtcatcgt ccgggtacaa ttccttgaat tgggcgaacg tgaattttgc tttggccatg
      661 gcgagatctc cctatggcca gaaggttact ccactatcta cttgctgtca agggataaag
      721 gcccgatcat aggggcggcg gagaaacaat atgtggctag cctctgaaat ggcggcgttc
      781 gctcacgtcg cagaactgaa atcgtttact gccgccgcgc gggtgctggg cgtccccaag
      841 gttgccgtga gtcgcgccat agtaagcctt gagcggcggc tcggcacccg gctgctgacg
      901 cgcacgaccc ggcgggtcgc gctgacgaat gccggtctgc tgcttcgtcc gcattgccag
      961 cggctggtgg ccgaagtcga cgccgttcgc atgcgtttcg cacccgccac cgacagcgtg
     1021 cgcagcctga ggctgatcgt ggacccgggc tacggccgac tgctggtcgc gccactggtg
     1081 ccccgctttc tcgagcgcta cggcgacttt caactgcagg tggcgctggt gaactcgatg
     1141 cctgaagagg cgaacaagga ctgggacttg ctgatttgca atgccgcccc ggagcggcca
     1201 gatctgattt gcaccgcact gggcaacccc tcgctgctgc tgtgcgcgac accgctttat
     1261 ctcgcgcgcc agggtcgtcc tcgcgcgccc accgatctga gcctgcactc ggtactgcgc
     1321 gtggttgcca ccactgaagc ggagggcggc gcaagcctgc gattgcggcg cgactgcgag
     1381 gagcagcaga ttcgcctcaa gcccgctttg caggtcaacg atcccgcgtt ggtccattcg
     1441 agcactgtcg ccagtttggg catcggcgtg ttgccggaat tcatgtgccg tcagggtctg
     1501 gcgatgggaa agctggagcg tgtgctcccg gactggtcac tggccgcacc gctgcggctt
     1561 caggccgtct atccaaccga gctggcaggg tcggccgcga tccgcctgtt catcgaattt
     1621 ctgctcgcga atatggcgcc ggtgctcggc ggcgatgccg gctagaccag cagttgcggc
     1681 tcggcatccg aaacgcaggc tccgcctgac ttgatgactc gcagcaagct cagcgactcg
     1741 ggcaacaggt gctcggcgta gaaacgcgcg gtttgcagtt tggcccgata gaagtccgga
     1801 tctgcagtgc cgtctgacag ccgcgcgttc gccagcgcgg cactgcgcgc cagcagtgct
     1861 cctccgagca cacgtccgca gagcatcaga tacggcacgg ataccgcata ggcgcgtccg
     1921 gcgtcctcac ccatctgata cagcaacact tcagtcgcat cgcgcaacac cgtgatcgcc
     1981 tcaatccccg aattgcgcac gctgcgcagg gtggcgtcgg tgccgcgcag atcattcagc
     2041 tcgcgcagcg tatcgcgcag caacgcggcc atcgccgcgc cacgatcgcg accgagcttg
     2101 cggccaagca gatcgttcgc ctgaatagcg gtggtgcctt cgtagatagc ggcgatgcga
     2161 gcatcgcgca gcgtctgtgc cacaccggtt tcctcgatga aacccatccc accgtgcacc
     2221 tgaatggcca gcgatgccat ttgcgtggcc tgctccgtgc accatccctt cacgatcgga
     2281 atcagcaatt cacctcgcgt cagagcggtg gcacgctgcg cttcgtcggg gtgagcgcgc
     2341 gccagatcga gttggagcgc agcgctcagc atcagtgccc gcatggcttc gacgttggcg
     2401 cgcagggtca gcatcatccg gcgcacgtcc gggtgataca cgatcggcac aggctgctcc
     2461 acgcgtatcc cgatcggacg accctggata cgactgtgcg cccaggcagc cgcctgctgt
     2521 cgcgccaact ccgacaatcc gaccccctga atacccactg acagacgtgc cgcgttcatc
     2581 atgatgaaca tgtattccag tccacgatgc ggctggccta ccagataccc gaatgcgccg
     2641 ccggcatcgc cgtacgccat cacgcaggtc gggctggcgt ggatgccaag cttgtgttcg
     2701 atcgacacac agcgcacgtc gttgcgtgca ccgagtgtgc cgtctgcccg gatcagaaac
     2761 ttggggaccg cgaacaatga gatgcccttg atgcccgacg gcgcaccgtc gattcgcgcc
     2821 agcaccaggt gcacgatgtt cggcgtgaga tcgtgttctc cgtaagtgat gaaaatcttc
     2881 tgcccggtga tgcgaaagtg atcggcgtca ggggtcgcgc gggtgcggat ggctgccaga
     2941 tcggagccag cctgtggctc agtgagattc atggtgccag tccattcgcc gctgacgatt
     3001 ctgggcagca atagcgattg cagctgcttg gaggcgaccg tttcaatcgc ttccgcagcg
     3061 ctgcgactga gcagcggaca ggcggtgaat gccatattcg cgccaaagtc gatctcttcg
     3121 acggccgatg acaacaccag cggcgcaccc tggcctccga actcggtggg cgcggaaagc
     3181 tgtgtccaac cgccttcgac gaagcgccga taggcgttgc ggaactcggt gggcatctgc
     3241 accgcgcctt cacgccagct cgcgcccact cgatcgccgg tgcggttgat gggcgccagc
     3301 acgtcctgtg caaagtttgc ggcttcttcc agtaccgcac cggtcagttc gatggaaaag
     3361 tcgcgatagc gaggcagttc ggccaggcgc gcgaccccca gcagatcgtc cagcacgaaa
     3421 cgcagatctc gtaagggtgc ctgatacatg gtgcaacggt ccgaaaaacg ctgagcggat
     3481 cacgctttca gcgcaatatg acaagtgtac gccgccagcc tgccgacagc ttgacagccc
     3541 gaaaaaaaca gcctcgaaga acgcgagcgc tgtcacgccg ctgcagtgcg aggcctgaga
     3601 cgattgggaa tgatgggttt tctcgacaca ccaccgtctg ccaagaacgg cgcgaatgcg
     3661 accgccagcg gctctgcgcc tgcggagagc cgtgagaggc gtttcaacac cgatgacatc
     3721 agcaaacaac tgctgcgcat cctgcagccg ttgcatgttg acagcctgtc gttacacgac
     3781 ggcgccggcg aactgctgtg gctcaacgaa ggtgcatttg gaccggacga gcatggcgtt
     3841 gtgctcgatg cgctagacgt gttcgccctg gatgcggatc gtgaagatct gcagctgaaa
     3901 ctcgacgacg accgcaccgc gctgtgtgct tgcgcgcgca gtggtggcgg tgaggtgctc
     3961 ggcatcgcat tgaccatcgt cgagaccccc gcgagtgaag aattcgccgc gaaactggct
     4021 gcaccgcgac tgagcggact catgcgccgt ctggcgcagc tgctcgcgcc gagctcaccg
     4081 gccgcgcccc acgccaccgg gccggccgca gcggcctctg cggtgccggc tgcatcgaag
     4141 aagcgcgccg aggcgatctc attttcggat agcgaggaca ttaccgagga gctggaagcc
     4201 gacccgcggc cgcggcaggt ggtggtaccg cgcgagcggc gaacgcagcc cgctccgagt
     4261 gccaaggtcg ctgcgcgcag cggcacgatt catgcccgtc gctatgcgcg gctgcgcgcc
     4321 ggcggtgcgg cccgacgcta tgaagtgaag ctggccccga acgcgtcgtt tgccgcggat
     4381 ttcacgcttg caacgcgcgt cgtggagcat ctgcagcgct caggcgatcg ctatacgcag
     4441 actccttcca gctttgccat tccactgagc acgcaatcgg ttacccaggc cggctggctt
     4501 tcgaagctgc agccgatcct ggcgcgcgcc aacctgcccg accatctcat tgggttctgt
     4561 ctgccgccgg cggcctggga ccaggaattg gaggccacgc gcagattcat tggtgagtgc
     4621 gaggagcagc gctgctttgt tgcgctggat gacttcacgc tcggaagcta cggcctgacg
     4681 ctgctgcaat ccaccgcggt caaatgccta aagctcgacg gcatcctgat cagctcagtg
     4741 gtgaacgaca aattcgccca cgcgacgctc gcggcgatcg tacaggcggc gcgggtgctc
     4801 ggcctgtact gcgtcgccaa gcaggtatcg tcagcgtcgg atgtgaagtg gctggctgcc
     4861 gctggaatcg aattcgcgga cggcgtcagt cgcggcctag cagtcgccgc caccatgaag
     4921 agcgatgatc tgtcggtgca tctgggcgag tagtgtttgg agcggccgct ggcgcgggct
     4981 tcacccctgg cgctttcatc gcagctttcg ctgcagcggc tggcgctgcg cgcttggcag
     5041 ttcccgcatt ggtggaataa ggatcgaatc cgactttgcg gctgtcgatt gaagatttct
     5101 tcttggactt gtccgaagcc gcagcgctgc tcgcaggttt ggcaggcgat gcttccggac
     5161 gagcaacctc ctcccctgct tcagattcca gcgacagacc ggtcagatcg agcttgcgta
     5221 acaattgcga ggtcgacgcc atggcggtgc gcgccgtgtc agcggcccaa tgccatacgg
     5281 cattgccacg gtcatcgtgc tgcacctgac cggtgttctg ctgtccgact tccggtcgct
     5341 tgtcgtcttt attcgccaaa gccttgcacc atctgcagat cggaaaacac tagcaaggcg
     5401 taacagtaga gcaaaggatc cagctctcgc cgccatgtgc tatggttaat ttggcacaaa
     5461 aatgagaact ggttcgcggt cctttcagtt cggccctcga attgtgagcg ccgcctacgc
     5521 ctgttatccc gtccgacggc accatagcga gcgatgctag ctgcgtcacc taaccctccg
     5581 gatcaggacg aactaccgcc gctgatgccg ccaccggccc ctgctgcgcc gccaccgtcc
     5641 gcagcggccg cgccggcgcc ggcccctggc cgtatcgacc gcgaattcgt cgtcaagaac
     5701 cgtctggtgg agcgctacat cggcggtcgc ctaccggtcc gaggagctca ggacctggag
     5761 cgtttctgcc gcgagcaccc tgatctgttg gatgaaattg cgctgaccga tcacatcaat
     5821 gctgcgctgc gtctactgga tgcgagcgga cgcgcgtcgc cgtgggaacc ggaacccaag
     5881 aaatggtggg aacagctctg ggtaccgatc gccgcggcga cgttggcgct gattctgggc
     5941 gtcacgagcc tggtgctgtc aggcaagctc ggcggacgcg accatacgat tgcacagctc
     6001 aagcagcagg tggctcagca gccgctggag ccaacgacct cgacacgttc gatcacggtc
     6061 atgcccagcc gggtggcgcc ctcgcgccac agcatggcga ccctcggtgg cgccgcgagc
     6121 gcggagatgg ccgatctgaa gatcgacgtg tcctggacac cgctggatca attccgggtc
     6181 acgatggatc gaatcgatca aggtcgcgtc gcgatctttc acaacctcaa gcgtgactcc
     6241 aacggcgtgc tgcatctgga actgaacagc tcggcattgg gtccgggcga ctatcagctc
     6301 acgatcgagg gactcgatcg ttggggcaat ccaatccctc aggcctgggc gacgatttca
     6361 atcgttcact gatcggcgcg ctcgctagta cagcaccgcg cgcggcggga cgcgcaccac
     6421 ggatcgcgtt gccagggttc cagccgttgc gaccacgatc agtgcgccga acaccccgat
     6481 gctccacagc caagcgttga agcgatagtt gaggtcgagc gagtgcgcca gccaggcgcc
     6541 gccgaccgag gctccgcacg ccgccaacag ccccgcgagt gcgccgatca gtgcgaactc
     6601 gaccagcatg ctggtgaaga tcatcgagcg gcgcgccccc aatacccgca atacggcggt
     6661 ctcatacagt cgttcttcgc ggctcgcctg aaccgcggcc agcagcaggg tcagtcctgc
     6721 gagcagcgta aacaggaata cgctctgcac agcggttacc gctttgtcga tcaccgcgcg
     6781 cacctgagcc agcagatccc cgacattgaa gatcgatacg cctgggaaac ggtgcaccaa
     6841 ctgcgccatc gcgccgccgc tcggctgcag gaaggcgctg gtcatgtagg tgcctgccgt
     6901 cccctcgagc acccctggcg cgaataccac gaaaaaattc ggccggaagc tgtcccattt
     6961 gacgtcgcga aaactgctga ccgtgacgtc gaagctctcg ccggcgacat cgaatcgcag
     7021 acggtcgccg agcttcaggc ccagcgaatc gcgatactcc atcgacagcg acacccaggg
     7081 tttgccgcgg tccgcggcgc tccaccactg
//
LOCUS       DATMRM010000316         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2967715, whole genome shotgun sequence.
ACCESSION   DATMRM010000316 DATMRM010000000
VERSION     DATMRM010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2967715"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /locus_tag="VNZ06_09430"
     CDS             complement(<1..1599)
                     /locus_tag="VNZ06_09430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HWW21012.1"
                     /translation="MTDFNTILGDRGPLAGTLEGFRARPAQQQMAAHIDAALRSRQHL
                     LVEAGTGTGKTYAYLVPALLSGLRVLISTGTRTLQDQLYHRDLPMLAGALGRPAQVTL
                     LKGRSNYLCRARFADIGRQEELLTASTDPLIGRLRDWSLATRSGDLSELPELADAHPL
                     RPQISSTRENCTGARCSEFSRCHVVEARRAAMEADIVVVNHHLLLADLALKEDGFGDL
                     LPSVDAVILDEAHQLPDLASEFFGVSVGSRQIEVLIADLKRELSVAGLSLRAATGVTA
                     GIEHCLTSARTRLGRTERSYRWEELGEEVREAFDALRRVLLELAAQLETSGGTAEVQQ
                     CAVRARAVASALGQIVEQQGGEEPALAGARTLTTQARGFSLQLLPYEIADRFRALVSK
                     AGNAWVFTSATLAVGEDFSHFANRLGLRDAATLRFDSPFDYAQQALLYLPSEMPDPGD
                     VEYGDAVIAMALPLIERAEGGAFLLFTSHRALRAAAQRLQSQPSESLPLLIQGSAPRE
                     QLLRRFRASGNAVLLGTASFWEGVD"
     gene            1738..>2914
                     /locus_tag="VNZ06_09435"
     CDS             1738..>2914
                     /locus_tag="VNZ06_09435"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HWW21013.1"
                     /translation="MATAPHTLASLSDVSKSVTDWVASVQELTQPEKIHWCDGSQMEF
                     QRLRGELVAKGELHELNQKTFPDCHLYRSDPSDVARVEHLTFICTRSKEDAGPNNNWM
                     DPLEARAKMRELFKGCMKGRTMYIVPYCMGPLDSAYSRCGVEITDSAYVVLNMAIMTR
                     IGRQALERIATDGTFVKGLHSIGELNPARRFIMHFPEDLSIESFGSGYGGNALLGKKC
                     HALRIASYQARTEGWLAEHMLIVGLQSPKGETHYVAAAFPSACGKTNLAMLIPPDTMP
                     GWKVFTVGDDIAWLHPGPDGRLWAINPEAGYFGVVPGTNPKTNRNAYETIRRHTLFTN
                     VAVTADNEPWWEGRTDGTPVTDWIGRPYDPKNGPAAHPNARFTVAATENPAYTKAAQD
                     "
BASE COUNT          581 a          949 c          876 g          508 t
ORIGIN      
        1 gtccacgccc tcccaaaaac tcgcggtacc cagcaacacc gcattgccac tggcgcggaa
       61 ccggcgcagc agctgctcgc gcggcgcgct accctggatc agcagtggca agctctcgga
      121 cggctgactt tgcagccgct gtgccgcagc gcgcagcgcg cggtgactgg tgaacagcaa
      181 aaacgcgccg ccctcggccc gctcgatcag cggcagcgcc atggcgatca cggcatcgcc
      241 gtattccaca tcacccggat ccggcatctc ggaaggcagg tacagcagtg cctgctgcgc
      301 gtagtcaaat gggctgtcaa agcgcagcgt ggccgcatcg cgcagtccca gccggttcgc
      361 gaaatgactg aaatcctctc ccaccgcgag ggtcgcggag gtgaacaccc aggcattgcc
      421 cgccttggag accagtgccc gaaagcggtc ggcaatttca tagggcagca gttgcaacga
      481 gaatccgcgc gcctgagtcg tcagagtccg cgcaccggcc agcgccggct cctcaccgcc
      541 ttgctgttcg acaatctgtc cgagtgccga agcgaccgca cgggcgcgca ctgcgcattg
      601 ctgcacttcc gcagtaccgc cgctcgtctc gagctgtgcg gccaactcga gcagcacacg
      661 gcgcaatgca tcaaaggcct cgcgcacctc ctcccccaac tcctcccagc gataacttct
      721 ctccgtccga ccaagccggg tgcgggcact cgtcaagcag tgctcgatcc cggcggtcac
      781 cccggtggcc gcacgcagcg acaatccagc cacgcttagt tcgcgcttga gatccgcgat
      841 cagcacctcg atctggcggc tgccgacact gacgccgaag aactcgctcg caagatcggg
      901 tagctggtgc gcttcatcca ggatcaccgc atcgacactg ggcagcagat cgccaaaccc
      961 gtcctccttg agcgcgaggt cggcaagcag cagatgatga ttcaccacga caatatccgc
     1021 ttccatcgct gcccggcgcg cctcgaccac gtggcaacgc gagaactcgc tgcagcgcgc
     1081 accggtacag ttctcgcggg tcgagctgat ctgcggccgc agcggatgcg cgtcggcgag
     1141 ttccgggagc tccgacagat cgccgctgcg cgtggcgagt gaccagtcgc gcagccgtcc
     1201 gatcagtggg tcggtcgatg cggtcaacag ctcttcctgt cgtcctatgt cggcgaagcg
     1261 cgcgcgacac agataattgg accgaccctt gagaagtgtc acctgggcgg gtctgcccaa
     1321 agcgcctgcc agcatcggca gatcgcggtg gtagagttgg tcctgcaaag tacgggttcc
     1381 ggtggagatc aacactcgca gtcccgatag cagtgcggga accagatagg cgtatgtctt
     1441 gcccgtgccg gtaccggctt ccaccagcag gtgttggcgt gagcgcagcg cggcgtcaat
     1501 atgggcggcc atctgttgct gtgcggggcg cgcccggaag ccttccagcg tccccgccag
     1561 aggcccccga tcgccaagaa tggtattgaa atcagtcatc tatcttgcat ttgacttatg
     1621 gaacaaactg ttcgcacacc aaattatgtt gcggggccgc gaaagcgccg caagtgacga
     1681 tcactcggta taattccgct tcccacgttt gtatcaacaa gaacctgagg atacgttatg
     1741 gcgacagcgc cccatactct cgcttccctg tcagatgttt caaaaagcgt cacggattgg
     1801 gtcgcgagcg tccaggagct gacacagccg gaaaagatcc actggtgcga cgggtcacaa
     1861 atggagtttc agcggctgcg cggggagctg gtcgccaagg gcgagctgca cgaactcaac
     1921 cagaaaacgt tccccgactg ccatctgtac cgatccgacc cgagcgatgt agctcgggtc
     1981 gagcacctca cctttatctg cacccggtcc aaagaggatg cggggccgaa caacaactgg
     2041 atggacccgc tcgaagctcg cgccaaaatg cgcgaactct tcaaagggtg catgaagggt
     2101 cgcacgatgt acatcgtgcc ctattgtatg ggtccgttgg actcagcgta ttcgcgttgc
     2161 ggcgtggaga tcaccgacag cgcctacgtg gtgctcaaca tggcgatcat gacgcgcatc
     2221 ggacgtcagg cgctggagcg tatcgccacc gatggtacct tcgtgaaggg tctgcattcg
     2281 atcggtgagc tgaacccggc gcgccgcttc atcatgcact tcccggaaga tctgtcgatc
     2341 gagagtttcg gctcgggata tggcggcaac gcgctgctcg gcaagaagtg ccacgcattg
     2401 cgcatcgcca gctatcaggc gcgcaccgag ggctggctcg ccgagcacat gctcatcgtc
     2461 ggactgcaaa gcccgaaggg tgaaacgcac tatgtggccg cggcgttccc ttccgcctgc
     2521 ggtaagacca atcttgcgat gctgatccca ccggatacga tgccgggctg gaaggtattc
     2581 acggtcggcg acgacatcgc ctggctgcac ccgggcccgg atggtcgtct gtgggccatc
     2641 aatccggaag ccggttattt cggagtcgtc ccgggcacca atccgaagac caatcgcaac
     2701 gcctacgaga ccatccgtcg tcacacgctg tttaccaatg ttgcggtaac cgctgacaac
     2761 gaaccctggt gggagggccg caccgatggc acgccggtga ccgactggat cggccgtccg
     2821 tatgatccga agaacggccc ggccgcgcat ccgaatgcgc gcttcacggt ggccgcgacc
     2881 gagaaccccg cctataccaa ggctgcacag gatc
//
LOCUS       DATMRM010000317         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4214551, whole genome shotgun sequence.
ACCESSION   DATMRM010000317 DATMRM010000000
VERSION     DATMRM010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4214551"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1806
                     /locus_tag="VNZ06_09440"
     CDS             187..1806
                     /locus_tag="VNZ06_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003123890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HWW21014.1"
                     /translation="MSNISALDDAARGMSTPAKPSKLSGHKTNPWFNVLAIIVVLIGI
                     VYAGYHLADDLSVVRDTRVYPYILLGVALFIALGFEFVNGFHDTANAVATVIYTNSMS
                     ANLAVLWSGTFNFLGVLTSTGAVAFTIVSLLPVELILQVGSGAGFDMVFALLIAAILW
                     NLGTWYFGLPSSSSHTLIGSIIGVGLMNQLMAASTDGTSGVDWSQATKIGETLLVSPL
                     IGFSAAWLLLSILKAVIKIPTLYKEPEGNKPPPVWIRGLLILTCTGVSFFHGGNDGQK
                     GMGLIMLILIGTVPTAYALNRTMHEQDVTTFMAASAQAQTALSHYGTGTTPADPRADV
                     LEMVRTRTVTAQGIPALAAMTATIAKQVGDYGSMDKVPPGSMINIRNQMYLVSEGLRF
                     MGKAKTLNVSEADQKSLGAYKKQLDRSTKFIPTWVKVAVAIALGLGTMIGWKRIVVTV
                     GERIGKTHLTYAQGASAELVAMATIGAAEMYGLPVSTTHVLSSGVAGTMVANHSGLQW
                     STVRNLLMAWVLTLPASIAISGGLYWLLRTL"
     gene            complement(1771..>2208)
                     /locus_tag="VNZ06_09445"
     CDS             complement(1771..>2208)
                     /locus_tag="VNZ06_09445"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HWW21015.1"
                     /translation="AEAMLRHLAAQEAPRLNLEIDSAGTADYHIGAPPDPRSQRAALQ
                     RGIDISGLQARQVTERDFMRFDLILAMDRANLRELESLKPAGANAQLKLFMEYAPGSV
                     VLEVPDPYYGDAAAFEVVLDLCAAASRGLLACLERAQQPIKAP"
BASE COUNT          446 a          659 c          677 g          426 t
ORIGIN      
        1 ccggcgccat tgccccgttc actgcggccg acagcaactg ccggcgcgcg agcagacgcg
       61 agaatggcag aacggcggcg gcatgagaga gagtgaaggg catgaaaaac tttggctgtc
      121 atccaactgt aataactcgg acctataagt tccggctcgg ccccattttc aactggatac
      181 aaaataatga gcaacatctc cgcgctcgac gacgcggccc gaggaatgag cacgccggcc
      241 aagccctcga agctcagcgg gcataaaacc aatccctggt tcaatgtgct ggccatcatc
      301 gtggtcctca tcgggatcgt atacgccggc tatcacctgg cggacgatct atcggtggtg
      361 cgcgacaccc gggtgtatcc ctacatactg ctgggtgtgg cattgttcat cgcgttggga
      421 ttcgagttcg tcaacggctt ccacgatacg gcaaatgcgg tcgccaccgt gatctatacg
      481 aactcgatgt cggcgaatct ggcggttctc tggtccggca cattcaattt cctgggcgtg
      541 ctgacctcga ccggcgccgt cgccttcacg atcgtgtcgc tgttgccggt cgaattgatc
      601 ctgcaagtgg gcagcggcgc cggattcgac atggtatttg cattgctgat tgccgccatt
      661 ctatggaatt tgggcacctg gtatttcgga ctgccctcat cgagttccca tacgctcatc
      721 ggctccatca tcggcgtcgg tctcatgaac cagctgatgg ccgcgagcac ggacggcacc
      781 agcggcgtgg attggagcca agccacgaag atcggcgaga ccctattggt ctcgccgctg
      841 atcggcttca gtgcggcgtg gttgctgctc tccatactca aggccgtgat caagatcccg
      901 acgctataca aagagccgga gggcaacaag ccgcccccgg tatggattcg cggcttgctg
      961 atcctgacct gtaccggcgt gagcttcttt catggcggga acgatggcca aaaaggcatg
     1021 ggactcatca tgctgatcct gatcggcacc gtgccgacgg cctatgcgct gaatcgcacc
     1081 atgcacgagc aagacgtgac taccttcatg gcggcctcag cgcaggcgca gacggcactt
     1141 tcgcactatg gaacgggcac gacgccggcc gatccgcgcg cagacgtgct ggagatggtg
     1201 cggacgcgta ccgtaacggc gcagggcata cctgcacttg ccgcaatgac cgcgacgatc
     1261 gcgaagcagg tcggcgacta cggttcgatg gacaaggtcc cgcccggatc catgatcaat
     1321 atccgcaatc agatgtacct ggtgagtgaa ggtctgcgct tcatgggtaa ggccaagacg
     1381 ctcaacgtat cggaagccga ccagaaatcg cttggcgcat acaagaaaca gctcgatcgc
     1441 tccacgaaat tcattccgac ctgggtcaag gtcgcggtgg ccatcgcatt ggggttgggc
     1501 acgatgatcg gctggaagcg aatcgtggtc acggtcggcg agcggatcgg caaaacgcat
     1561 ttgacctacg cacaaggcgc atcggcggaa ctcgttgcca tggcgaccat cggtgccgcc
     1621 gaaatgtacg gtttgccggt cagtaccacg cacgtgctgt cctcgggcgt cgccggaacc
     1681 atggtggcaa atcactcggg cctgcaatgg tcgaccgtgc gcaatctgct gatggcctgg
     1741 gtgctgacgc tgcccgcatc catcgcgata tcagggggcc tttattggct gctgcgcacg
     1801 ctctaggcag gcgagcagcc cgcgcgacgc ggcagcgcag agatcgagga ctacctcaaa
     1861 agcggccgcg tcgccgtagt aaggatccgg cacctcgagc actacggagc cgggtgcata
     1921 ctccatgaaa agcttgagct gagcgtttgc gccggcgggc ttcagtgact caagttcgcg
     1981 caggtttgcg cgatccatcg ccagaatgag atcgaagcgc atgaaatcgc gctcggtgac
     2041 ctgccgcgct tgcaatccac tgatatcgat gccgcgctgc aaggcggcac gttggctgcg
     2101 cggatccggc ggtgcgccga tatggtaatc agctgtgccc gcggaatcga tctcgaggtt
     2161 cagccgcggc gcttcctgcg cggcgagatg ccgcaacatg gcctccgc
//
LOCUS       DATMRM010000318        12972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3577163, whole genome shotgun sequence.
ACCESSION   DATMRM010000318 DATMRM010000000
VERSION     DATMRM010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12972
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3577163"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(354..494)
                     /locus_tag="VNZ06_09450"
     CDS             complement(354..494)
                     /locus_tag="VNZ06_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006750838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21016.1"
                     /translation="MKRLMILIPLTLLTLAGCVVAPARGYYYYHPRAVAVVPAPAIIV
                     RP"
     gene            complement(732..1061)
                     /locus_tag="VNZ06_09455"
     CDS             complement(732..1061)
                     /locus_tag="VNZ06_09455"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWW21017.1"
                     /translation="MARCGLGPRLPLNVISPWTKVHYVDHTVTDQTSVLRFVEDTFKL
                     DYIDGAAKFDPTNGARIPAFTLAPQRQSFDVITASLDNNFARVPNPARFFLDPVTGAV
                     LDDDDQR"
     gene            complement(1067..1837)
                     /locus_tag="VNZ06_09460"
     CDS             complement(1067..1837)
                     /locus_tag="VNZ06_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21018.1"
                     /translation="MRATSHRVPRLCCLLSLLGLVPQVKAQTDEIQVYDAQIVELGHW
                     NLTWHNNFTPSGRLQPDFPGGVVPDHALNGVPEWAYGVTDWFEAGVYLPLYTLTNTGQ
                     LQFDGAKLRTLFVSSHAAERTVFYGVNFEYSYNRPQWESHRFAGEIRPIIGTHLGPLD
                     LIVNPIVDTQYNGLRNLEFVPAERVAYNFPRFSLALEHYADFGRLRHFAPLAQQSQTL
                     FAVIDIGNSSWGLEFGVGHGFTAASDDLIIKLLLMRDL"
     gene            complement(1864..2523)
                     /locus_tag="VNZ06_09465"
     CDS             complement(1864..2523)
                     /locus_tag="VNZ06_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWW21019.1"
                     /translation="MPAELMPRESRNRAGALWRALRGSLMMLPLLAHAGGGPFGIDHE
                     LRRSDTGIWSRDVQTGLEEGVIVFDVAGALWLGNDQPFGHTLWQSADAMTISAIGAQL
                     LKYAFSRARPDQGDNPDAWFKGRGHQSFPSGEVTEQASFVTPFIIDYQREHPWVWALE
                     LLPAYDSVARLKSQAHWQSDVIAGWLLGSGIGYWAATRRTPLFVQVLPRGVTVGFYKR
                     L"
     gene            complement(2513..4426)
                     /locus_tag="VNZ06_09470"
     CDS             complement(2513..4426)
                     /locus_tag="VNZ06_09470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWW21020.1"
                     /translation="MPRPALAAALAALSISAMAAAQTPPSTQISEIPDNFVMPTAQND
                     YVKRIEMIAMRDGVRLNTVIIIPKGASHAPIVLTRTPYSAKERAANDNPSIYELLPET
                     DQDFARAGYIRVFQDIRGKYGSEGAFEMTRPPRGALNGTTTDETTDAWDTIDWLVKNL
                     PQSNGKVGMFGSSYEGFTVVMALLNPHPALKAAVPESPMVDGWMGDDFYHYGAFRQPN
                     LDYFAAQTEQRGSGKSVPRPAHDDYEVFLDAASVGAYAVSSGFTQLGWWNKMAAHPAY
                     DAFWQELALDRQLAAHPSNVPTLWEQGLWDQEDMWGANHAWLALKAAGHESNNWLVLG
                     PWYHSQANHFGWNTGVLKWPGDTAEQFRHDIVMPFFNQYLKDGPPANLARVIIYDPPR
                     GRWEDFKTWPSACESGCAHPMKALYLQADFGLSFDRPGGSHQGDSYVSDPAKPVPYVP
                     RPIQFSDRPAWQTWLVRDQRFVDGRPDVLSYESAPLTQALKIEGAPIADIVAQTTGTD
                     ADFVVKLIDVYPPQYPMQPELGGYELPIGIDIFRGRYRASFEHPAPIPANKSQHYRFT
                     LPNQNYVFLPGHRVMVQIQSTLFPLYDRNAQTYVDNPLFPKASDFRKATITILRSSQE
                     ASAVWLPVVPDAG"
     gene            4486..5586
                     /locus_tag="VNZ06_09475"
     CDS             4486..5586
                     /locus_tag="VNZ06_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HWW21021.1"
                     /translation="MSISLQRLLPLFERERQAGHAMVMATVLRTAGPTYSKSGALMLI
                     THSGEYAGILTGGCLEGDLSERSRAVLSQGSAVVTSYDMRGPDDLLFGLGSGCEGAMD
                     ILLQRLDPRGDWQPMDRLAQAWRAQRAASLVLVGRSGDSRWPSGSGVFAEDGVVFGTA
                     CADRAGAASLRVLAQQSLAQKSGRILAQALPGIDVLALYQERPSRILLLGGGPDAQPV
                     AELAAFLGWQITLIDHRPHYARSARFPAARAVLDTGPAGLRELLASEQAPFDSAIIMS
                     HHFATDLAYLRVVAETEIPYVGLLGPIVRRERLMAELGAGAARLRSRLRAPVGLDLGA
                     AGPESIALAIVAEIQGTLAGRESMGPMSRKQA"
     gene            5641..6105
                     /locus_tag="VNZ06_09480"
     CDS             5641..6105
                     /locus_tag="VNZ06_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWW21022.1"
                     /translation="MSYQVNINGTTHEVDADGDTPLLWVLRDVLGMTGTKFGCGVAQC
                     GACTVHANGTPMRSCQLALESVGKLSITTIEGIGATDLGKRVQQEWLSLDVPQCGYCQ
                     GGQILAATALLMHTPNPTDADIDKAMTGNICRCGCYTRIRKAIHNVASSLKA"
     gene            6119..8395
                     /locus_tag="VNZ06_09485"
     CDS             6119..8395
                     /locus_tag="VNZ06_09485"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWW21023.1"
                     /translation="MNHPSPLSEDVLSAGQVSRRQFLLASGLAGGGLLLTATIPSLGR
                     TAEAGAADAGAADAQITIYARIASSGTVTIFAPNPEMGQGTKTALPMIFAEELCVAWT
                     DVEIEMADYLGGKMGGQSSGGSFSTPSNWMPLRKAGAAGRDMLIGAAAQTWSVPAEEC
                     YAQDSQVIHRPSGRSLRYAQIAQKASTLPVPDMNKVVLKDESAFRIIGKSVVDPDKAR
                     VVVGKQTFGIDVKVPGMRYAVYQKGPVFDAEVKSANLEDIRRLPGVSHALVLKGAPPP
                     AGAQGMDDALRGGVAIVADTWWRAQKARRNLKVEWIEGAHAEDSTASFDAQAKKLFTQ
                     PPEKAVRVDGDPDGALKGAAKVVRAQYSYPFIAHVPMEPLNCVASWQDGKVEIWAPTQ
                     NPGPGRAGVARTLGIKPENVTVHLYRCGGGFGRRLANDYMIEAAAISKEIGAPVKVLY
                     AREDDIQHDLYRPGGYHDFSAGLDGAGALIAFHNHFVGFAKGDAISRLAMPGPEIFPA
                     GYVPNFALKTSKISFNIPIGPLRAPGDNAHAYVYQSFLDELAHAAGKDPIDFQIELLH
                     SPLPGEGEGKGGSPFGPGFLAKRMIAVLETVREMSGWKYRSSLPSGTGMGLGCFYSHL
                     GYVAQVHQVHVDSHGIITPQHVWVALDVGRHIINPTNAEHQVQGSVLDGISAALDQAI
                     TLEKGRVVQSNFHNYHLLRNGKIPTITTVFVKTDYAPTGLGEPAHPSALPAFCNAIYA
                     ASGKRVRKLPLSTAQFRI"
     gene            complement(8400..8825)
                     /locus_tag="VNZ06_09490"
     CDS             complement(8400..8825)
                     /locus_tag="VNZ06_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWW21024.1"
                     /translation="MILPDVNVLLYAFRADSENHARYKDWLETVVNGQGAYAISPQAL
                     SGVIRISTHPRIFARPSSRADAFSFCRQLLEQTHATIVAPGDRHWEIFEELCETSKPT
                     GNLIQDAWFAALAIEAGCEWITSDRDFARFPGLTWRAPF"
     gene            complement(8822..9067)
                     /locus_tag="VNZ06_09495"
     CDS             complement(8822..9067)
                     /locus_tag="VNZ06_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2191 domain-containing protein"
                     /protein_id="HWW21025.1"
                     /translation="MRTTVRLDQALMERAKAEAARRHTTLTSLIEEGLELLLRKPLKR
                     TRRPPIKLTECRAGGGTLPGIDLNDSASLLDRLEGRD"
     gene            9241..9867
                     /locus_tag="VNZ06_09500"
     CDS             9241..9867
                     /locus_tag="VNZ06_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWW21026.1"
                     /translation="MTNSRQRAIETAERLFRSQGYAATGLTQILHESGSPKGSFYFHF
                     PNGKHELALATLESYGQRVDGNIRAIALEHARDPIGFVRSLARFVAREMESSGWSLGC
                     LNHKLASELVPGDPAITEVTANVYRGWVTQVAYALSGIDGATRKDAERLAMSLLAGLA
                     GARGLARIYRSPEPFEAVAQGMIDAMRSFAVGHPRSAASVRRRKAHSR"
     gene            complement(9934..10608)
                     /locus_tag="VNZ06_09505"
     CDS             complement(9934..10608)
                     /locus_tag="VNZ06_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21027.1"
                     /translation="MGRTWLIVLGLAAAAAIGLYAWTFGPNTAWLPSAHTQDWARFGE
                     FVGGVFGMLALVGVLVTVEQQRRTLEWHQNQTTLDELLREARDLATIVETILAARVET
                     VALTAQQLSVLEKPSTVSGVLELVDARAASEVGRPMPPPAENKSAYRKAVAGSIAGLS
                     QKLDLLAAVLSEFVSRGGDTIFLLFYRDRFRATVQRLIGLEIAVSTGDWWIQSSEESE
                     SRRWRF"
     gene            10635..11054
                     /locus_tag="VNZ06_09510"
     CDS             10635..11054
                     /locus_tag="VNZ06_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21028.1"
                     /translation="MDAVKSLGKKAQGARLERMQASPRWLGERFANLHPIAPGLRDPN
                     TPMPTLGEFICGGGRRVPAAPLPALNPLVWWKRPPDSGLRATWLGHSTLLLEMGGYRV
                     LTDPVWGPRASPSRLLGPKRFSRFRYVCAPCRSSIWS"
     gene            11030..11767
                     /locus_tag="VNZ06_09515"
     CDS             11030..11767
                     /locus_tag="VNZ06_09515"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWW21029.1"
                     /translation="MPQLDLVVISHDHYDHLDYPTIRELARHPVPFVTSLGVGAHLEA
                     WGVPPERIVELDWWETHTVPNTGLSVTAAPSQHFSGRGLKDRNATLWSSMVIRSPRHA
                     VFFSGDTGLTSEYRSIGERLGPFDLVMLEVGAFHPAWGDIHLGPENALRALKFLGSGT
                     FLPIHWGTFALAMHAWDQPAEVMFDLAPKADVRLLMPRLGEPVEPVQVGAVTPWWRGV
                     DTMQASVPHPDEPMPMPIPKTMSWPID"
     gene            11803..12885
                     /locus_tag="VNZ06_09520"
     CDS             11803..12885
                     /locus_tag="VNZ06_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWW21030.1"
                     /translation="MKFRLAMLCLLAPLALIALLRAGSALADDAKRLRLITWSDYAPA
                     EVIAQFKQESGIQVEVTLSNNEEIISKLRATGGAGYDLAQPSQDRITGVQRDFGVYKP
                     IDLSQVKLDQFRSELLDITRKNATLDGKLYALPYVWGTDDLVVNTRAAKLADYTDLCR
                     ADLKGKSSVRLRRPTLMAFAFASGKDPFALYADSKAYAALMDSVGQTLSACKANVRFF
                     YDNKDQLLNAMRSGEVAAAMAWDTEGWRLNRENADIKVIVPRSGALGWMDTFALPARG
                     RNDAGAYAWINFTMRPDIAARITKSVGNWTAATGTETLVDATLRAQYNATFPEGAFKN
                     IHWYPAIPSGLEEIEGRVLDRIKAAR"
BASE COUNT         2327 a         4056 c         4260 g         2329 t
ORIGIN      
        1 gggcaatcgc ggtgggtgat cgctgctgcg accggatcag aggcaaggtt ggtgctggca
       61 agcgaacttg agatcgccag tagcccgatt agtcccgagt acaccgatgt gcgaacgcag
      121 tgacgcatgc tgaatttcca cagatcgcag ataaagcgaa agcgtcgcag gtgcgaggcg
      181 ccgcggcaag agggcaagag gggtagatga tgacccggta ggtaagcggt gtgctgcgga
      241 tcgcgctcag agagcgctta cagattatct gccgattgcg ccggggagag ggcccagacg
      301 atgtccctcc atcccggcgc gtactggtga cggcgctgca tggcgggtgt tgcttacggc
      361 cggacgatga tcgcaggggc cggcaccacc gctaccgcgc gcggatggta atagtagtaa
      421 ccccgggctg gcgccaccac gcaaccggcc agtgtcagca gggtcagcgg gatcaatatc
      481 attaaacgct tcatggtccg agactccttt agcaagtacg aatgagcttc ctatgagcgt
      541 tcaacgcatc gcccgctagc gggctgacag tggcgcgacc cttgtgtcgg tgtcacgaca
      601 atttgcctgg gggccgcgga ttatgtcccg ccccgaccga ccggtaggga cggccaatgc
      661 aaatcagtag cacaaaaaaa accgccgccg gcttggcgcc ggcagcgggc aggtggggcc
      721 agttctacga cttagcgctg atcatcgtcg tcaagcaccg caccggtgac cggatcgagg
      781 aagaagcgag ctgggttcgg gacgcgcgca aagttgttgt cgagcgaagc ggtgatcacg
      841 tcgaacgact gacgctgcgg agccagcgtg aaggctggaa tgcgcgcgcc gttggtcgga
      901 tcgaatttcg ccgcaccgtc gatgtaatcc agcttgaacg tgtcctcgac aaagcgcagg
      961 accgaggtct ggtcggtcac ggtgtgatcg acgtagtgga ccttggtcca cggcgagatg
     1021 acgttcagcg gcaggcgcgg tccgaggccg cagcgagcca cccgcgctac aggtcgcgca
     1081 tcagcagcag cttgatgatg agatcgtcgc tcgcggcggt gaaaccgtgg ccgacaccga
     1141 actccagtcc ccaggaactg ttgccgatgt cgatcacggc gaacagcgtc tgcgactgct
     1201 gtgccaacgg cgcgaagtga cgcaagcggc cgaagtccgc atagtgttcc agcgccaggc
     1261 tgaacctcgg gaagttgtag gcgacacgct ccgccggtac gaattcgaga ttgcgcaagc
     1321 cgttgtactg ggtgtcaacg atcggattga cgatcagatc caggggcccc agatgtgtgc
     1381 cgatgatcgg tcggatctca ccggcgaagc ggtgcgactc ccactgcggg cggttgtagc
     1441 tgtactcgaa attgacgccg tagaacacgg tgcgttcggc ggcgtgcgaa gacacgaaca
     1501 gggtgcgcag cttcgcgcca tcgaactgca gttgaccggt gttcgtgagg gtgtagagcg
     1561 gcaaatagac gccggcttcg aaccagtcgg tgacaccgta cgcccattcg ggcacaccgt
     1621 tgagcgcgtg atcgggcacg acgccgccgg gaaagtccgg ctgcagacgt ccggacggcg
     1681 tgaagttgtt gtgccaggtg agattccagt ggccgagctc aacgatctgc gcatcgtaga
     1741 cctggatctc gtcggtctgc gctttgacct gcggcacgag ccccagcaac gacagtagac
     1801 agcacagccg cgggacacga tgggacgttg cgcgcacggc gttactttat atcagcgagt
     1861 cgttcagagg cgcttgtaga acccgaccgt cacgccacgc ggcagcacct gcacgaacag
     1921 cggcgtgcga cgcgtcgccg cccagtagcc gataccggac ccgaggagcc agccggcgat
     1981 cacgtccgat tgccagtgcg cctgactctt cagacgtgcg accgagtcgt aggcgggcag
     2041 caactccagg gcccacaccc acgggtgttc gcgctgatag tcgatgatga atggcgtcac
     2101 gaagcttgcc tgctcggtga cctcgccact ggggaagctc tggtgcccgc ggcccttgaa
     2161 ccaggcgtcg gggttgtcgc cctgatcggg gcgtgcgcgg ctgaacgcat atttgagcag
     2221 ctgcgcaccg atagccgata tcgtcatcgc gtcggcggac tgccagagcg tatggccgaa
     2281 gggctggtcg ttgccgagcc agagcgcccc ggccacatcg aagacgatca ctccctcttc
     2341 gagtccagtc tgcacgtcac ggctccagat gcccgtgtcg ctgcgccgaa gttcgtgatc
     2401 gatgccgaag ggtccgccgc cggcatgagc gagcagcggc agcatcatca gactgccccg
     2461 cagcgctcgc cagagcgcgc ctgcgcgatt gcgtgattcc cgaggcatca gctcagccgg
     2521 catcaggcac cacgggcagc cacaccgcgc tggcctcctg cgacgaacgc aggatcgtga
     2581 tcgtcgcctt gcgaaagtcg ctcgctttcg ggaacagcgg attgtccaca taggtttgcg
     2641 cgttgcggtc gtacagcggg aacagcgtcg actgaatctg caccatcacg cggtggccgg
     2701 gcaggaacac gtagttctga ttgggtagcg tgaagcggta gtgctgtgac ttgttcgccg
     2761 ggatcggcgc ggggtgctcg aagctcgcgc gatagcggcc gcggaaaata tcaataccga
     2821 tcggcagctc atagcctccc agctcaggct gcatcgggta ttgcggcgga tacacatcga
     2881 tgagcttgac cacgaagtca gcatccgtgc cggtggtctg cgccacgatg tcggcgatcg
     2941 gtgcgccctc gatcttcagc gcctgcgtga gtggcgcgct ctcatagctg agcacgtccg
     3001 ggcggccgtc cacgaagcgc tgatcgcgca ccagccaggt ctgccacgcg ggacggtcgg
     3061 agaactggat cgggcgcggc acatacggca ccggtttggc aggatcggac acgtaagagt
     3121 cgccctgatg tgagccgccg ggtcggtcga acgacaggcc gaaatccgcc tgcaggtaca
     3181 gcgccttcat cgggtgcgcg cagccgctct cacaggcgct cggccaggtt ttgaaatcct
     3241 cccagcgacc gcgcggcgga tcgtagatga tcacgcgcgc cagattcgcc ggcggtccat
     3301 ccttcaggta ttgattgaaa aacggcatca cgatgtcgtg acggaactgc tccgccgtgt
     3361 caccgggcca cttcagcacg ccggtattcc atccgaaatg attcgcctgg ctgtgatacc
     3421 agggacccag caccagccag ttgttggact cgtgcccggc cgctttcagt gcgagccagg
     3481 cgtgattggc gccccacatg tcttcctgat cccacagtcc ctgttcccac agggtgggta
     3541 cattgctcgg atgcgcggcg agctgcctat cgagcgcgag ctcctgccag aacgcatcgt
     3601 aggccggatg cgcggccatc ttgttccacc acccgagctg cgtgaagcca ctcgatacgg
     3661 cgtatgcccc gaccgatgcg gcgtccagga agacttcgta gtcatcgtgt gccggacggg
     3721 gtaccgattt tcccgacccg cgctgctcgg tttgtgcggc aaagtagtcg agattcggct
     3781 ggcgaaacgc gccgtaatga taaaaatcat ctcccatcca tccatccacc atcggactct
     3841 ccggtaccgc ggccttgagt gccggatgcg gattgagcag cgccatcacc accgtgaagc
     3901 cctcatagga agagccgaac attccgacct tgccgttcga ctgcggcagg ttcttcacca
     3961 gccagtcgat cgtgtcccac gcatcggtgg tctcatccgt tgtggtgccg ttgagcgctc
     4021 cacgtggcgg tcgcgtcatc tcaaaggcgc cttccgagcc gtacttgccg cggatgtcct
     4081 ggaatacgcg gatatagccg gcgcgcgcga agtcctggtc ggtctccggc agcaactcat
     4141 agatcgaggg attgtcgttc gcggcgcgct ccttcgcgct gtatggcgtt cgggtcagca
     4201 cgatcggcgc atggctcgcg cccttcggga tgatgatgac ggtgttgagc ctcaccccgt
     4261 cgcgcatcgc aatcatttcg atgcgtttga cgtagtcgtt ctgcgcggtc ggcatcacga
     4321 agttgtccgg gatttcgctg atctgcgtgc tcggcggcgt ctgcgccgca gccatggcgc
     4381 tgatcgacag cgccgcgagt gcagcggcga gggcaggcct gggcatgatt aacctctgtt
     4441 aacaatcaag gcttttctgc atgtgaaaca tcatacaatc agccggtgag tatttcgctg
     4501 caacgtctgt tgccattgtt tgagcgtgaa cgccaggctg gccatgcgat ggtgatggcg
     4561 acggtgctgc gcaccgccgg cccgacctac tccaagtccg gtgctctgat gctgatcacg
     4621 cacagcggcg aatatgcggg gattctcacc ggtggctgtc tggaagggga tctgtccgag
     4681 cgcagtcgcg cggtgctctc acagggaagc gcagtggtca cgagctatga catgcgcggc
     4741 ccggatgatc tgctgttcgg actgggcagc ggttgcgagg gggcgatgga tatcctgctg
     4801 cagcgcctgg atcctcgtgg ggactggcag ccgatggacc ggctggcaca ggcctggcgt
     4861 gcgcagcgtg cagcctccct ggtgctggtc gggcgctccg gcgattcgcg ctggcccagc
     4921 ggctcgggcg tgttcgcgga ggatggcgtg gtgtttggaa cggcttgcgc cgatcgggcg
     4981 ggcgccgcgt cactgcgcgt gctggcgcaa cagtccctgg cgcaaaaatc cggccgcatc
     5041 ctcgcccagg ccctcccggg aatcgatgtg ttggcactct atcaggagcg accctcgcgg
     5101 atcctgctgc tcggtggcgg tcctgacgcg cagccggtcg cggagctggc cgcatttctc
     5161 ggctggcaga tcacgctgat cgatcatcgc ccgcactatg cgcgctcggc acgctttcct
     5221 gcggcgcgcg cggtgctgga taccgggcct gcgggcctgc gcgagcttct ggcatcggag
     5281 caggcgccgt tcgatagcgc gatcatcatg agccatcatt ttgcgaccga tctggcctat
     5341 ctgagggtgg tcgccgaaac cgagattccg tacgtggggc tgcttggccc catcgtgcgt
     5401 cgtgagcgac tgatggccga actcggcgcc ggcgccgcgc ggttacgctc gcgcttgcgc
     5461 gctccggtgg gtctggacct gggggcggcg gggccggaga gcatcgcgct cgcgatcgtg
     5521 gcggaaattc agggcacgct cgccggccgt gagagcatgg gaccgatgag ccgcaaacag
     5581 gcatagatta gtaggacgga ggtatcatag gctgtatgcg cctgtgacga ggaggggatc
     5641 atgagctacc aggtcaacat caacggcacg acccatgagg tcgatgcgga cggcgacacc
     5701 ccgctgctct gggtgctgcg cgatgtgctc ggcatgaccg gcaccaagtt tggctgcggc
     5761 gtggcgcagt gtggggcctg cacggtgcat gcgaatggca ccccgatgcg ctcctgccag
     5821 ctggcgctcg agagcgttgg caagctgtcc atcacgacca tcgaaggcat cggcgcgacc
     5881 gacctcggca agcgcgtaca gcaggagtgg ctgtcgcttg atgtcccgca gtgcggctac
     5941 tgtcagggcg gtcagatcct tgcggccacc gcgttgctga tgcacacgcc gaatccgacc
     6001 gatgccgata tcgacaaggc aatgaccggc aacatctgtc gctgcggctg ctacacccgc
     6061 attcgcaagg ccatacacaa tgtcgccagc tcgctgaagg cctgaacgga ggcacaccat
     6121 gaatcacccg tcaccgcttt ctgaagacgt gttgtcggcc gggcaggtca gccgacgaca
     6181 gttcctgctc gcgagtggcc tggcgggcgg aggcctgctg ctgaccgcga cgattccctc
     6241 gctcggacgc acggcggagg ccggggcggc cgatgccggg gcggccgatg cgcagatcac
     6301 gatctacgca cgcatcgctt cgagcggcac cgtcacgatc ttcgcgccca atccggaaat
     6361 ggggcagggc accaagaccg cgttgccgat gattttcgct gaggagctgt gcgtcgcctg
     6421 gaccgacgtt gaaatcgaga tggccgacta tctgggcggc aagatgggcg gccagtcctc
     6481 gggcggcagc ttctccacgc cgtccaactg gatgccgctg cgcaaggccg gtgccgccgg
     6541 acgggacatg ttgatcggcg cggccgccca gacctggagc gtgccggcgg aggagtgtta
     6601 cgcgcaagac agccaggtga ttcatcgccc gagcggccgc tcgcttcggt acgcgcagat
     6661 cgcgcagaaa gcctcgaccc tgccggtgcc ggatatgaac aaggtcgtcc tcaaggacga
     6721 gtcggccttc aggatcatcg gcaagtcggt cgtggatcct gacaaggcgc gcgtggtcgt
     6781 gggcaaacag acctttggca tcgacgtgaa agtgccgggc atgcgctacg ccgtctacca
     6841 gaagggcccg gtgttcgatg ccgaggtgaa gagcgccaac ctcgaagaca tccggcggct
     6901 tccgggtgtc agtcatgcat tggtactgaa gggtgcgccg ccaccggccg gcgcacaggg
     6961 catggacgat gcgctgcgcg gcggcgtggc gatcgtggcc gatacctggt ggcgcgcgca
     7021 gaaagcgcgc cgcaacctca aggtcgagtg gatagagggt gcgcacgccg aggattccac
     7081 cgcctcgttc gatgcgcagg ccaagaagct gttcacgcag ccaccggaga aagccgtgcg
     7141 cgtggacggc gatccggacg gcgcgttgaa aggcgctgcc aaggtggtgc gcgcgcagta
     7201 ttcctatccg ttcatcgcgc acgtgccgat ggagccgctg aactgcgtgg cctcctggca
     7261 ggatggtaag gtcgagatct gggcgccaac gcagaacccg gggcccggtc gtgccggtgt
     7321 ggcccgtacg ctcggcatca agcccgagaa cgtcacggtt catctgtatc gctgcggcgg
     7381 cggttttggc cgccggctcg ccaacgacta catgatcgaa gcggccgcga tctccaagga
     7441 gatcggcgcg ccggtcaagg tgctctatgc ccgtgaggat gatatccagc acgatcttta
     7501 tcggcccggc ggctatcacg atttttctgc cgggctggat ggcgcagggg cgctgatcgc
     7561 gtttcataat cacttcgtcg gcttcgccaa gggcgatgcg atctctcggc tcgcgatgcc
     7621 tggtccggag atcttccccg ccggatatgt accgaacttc gcgctcaaga cctccaagat
     7681 ctccttcaac attccaatcg gtccgttgcg tgcgcccggt gacaacgcgc atgcctatgt
     7741 ttatcagtcg ttcctcgacg agctggcgca tgccgccggc aaggatccga tcgattttca
     7801 gattgagctg ctgcactcgc cgctgcccgg agaaggcgaa ggcaagggtg gcagtccgtt
     7861 cggtccgggc ttcctggcga agcggatgat tgcggtgctc gagaccgtgc gcgagatgtc
     7921 gggctggaaa taccgctcat cgctgccctc cggcaccggc atggggctgg gctgcttcta
     7981 cagccatctg ggctacgtcg cgcaggtgca tcaggtgcac gtcgattcgc atggcattat
     8041 cacgccgcag cacgtctggg tggcgctgga tgtcggccgg cacatcatca atccgaccaa
     8101 cgccgagcat caggtacagg gttccgttct cgacggcatc tcggccgcgc tcgatcaggc
     8161 gatcacgctc gagaagggcc gggtggtgca gagcaatttt cacaactacc acttgctgcg
     8221 caatggcaag atcccgacga tcacaacggt gttcgtgaag accgactacg cacccacagg
     8281 actgggcgag ccggcccacc cgtcggcact gccggcattc tgcaacgcga tctacgccgc
     8341 atccggcaag cgcgtacgca aactgccgct cagtaccgcg cagttcagga tctgaccgcc
     8401 tagaaaggcg cgcgccaggt gagtccgggg aagcgggcga aatcccggtc gctggtgatc
     8461 cattcgcagc cggcctcgat ggccagcgcg gcgaaccacg cgtcctggat caggtttccc
     8521 gtgggcttcg aagtttcgca aagctcttca aatatttccc agtgacggtc accgggcgca
     8581 acgatcgtag catgggtctg ctcgagcagc tgacgacaga aggaaaacgc gtcagccctc
     8641 gaactcggcc tggcaaagat ccgcggatgc gtgctgatcc ggatgacacc cgacagtgcc
     8701 tggggtgaga tcgcataggc accttgaccg ttgacgaccg tctccagcca gtccttgtag
     8761 cgagcgtggt tctccgaatc cgcacgaaag gcgtagagca gcacgttcac atcgggcagg
     8821 atcaatcgcg gccctccagg cgatccagca ggctggcgct gtcgttcaga tcgatacctg
     8881 gcaatgtacc cccaccggcc cgacattccg tcagcttgat gggaggtcgg cgggtgcgct
     8941 tcagaggttt tctgagcaat aactcgaggc cctcttcaat cagcgacgtc agtgtcgtat
     9001 gacggcgcgc agcctctgcc ttggcgcgtt ccatcagcgc ctggtccaat cttacggtgg
     9061 ttctcatgcg ccagagcata ccacatgata tgtcagtatg tctgagcggc cttcaggcaa
     9121 tgccggcgca aagcctgcga cgcgcttgaa catgacgata tgtcgctcgc gatagcgcga
     9181 ctcatgaatg taccgtcgcg agcaggctga cctataatcg gctcgacggg gtggggcacc
     9241 atgacaaact cgcgacagcg agccatcgag accgccgaaa ggttgtttcg ttcgcagggc
     9301 tatgcagcga ccggactgac gcagattctg catgagagtg gcagtccgaa gggctcgttc
     9361 tatttccatt ttccgaacgg caagcatgag ctggcgctcg cgacactcga gtcgtacggg
     9421 cagcgcgttg acggcaacat ccgcgcgatt gcactggagc acgcgcgcga tccgatcggt
     9481 tttgtacggt cactggcccg cttcgtggcg cgcgaaatgg agagttcggg ctggagtctc
     9541 ggctgcctga atcacaaact ggccagtgag ctggtgccgg gtgatccggc gatcaccgag
     9601 gtgaccgcaa acgtgtatcg gggctgggtg acgcaggtcg cctacgcctt gagtgggatc
     9661 gacggcgcga cccgcaagga tgccgaacga ctggcgatgt cactgctggc cgggctcgcc
     9721 ggcgcgcgcg gcctggcacg catctaccgc agtcctgaac cgttcgaggc cgtggcccag
     9781 ggcatgatcg atgcgatgcg ctcgtttgcc gtcggacacc cccgctcggc ggcgagcgtc
     9841 cggcgccgca aggcccattc acgctaggct gtaacccttc gcccgttcca cgggcatgta
     9901 cggcagcgcg tgctcacgct gcacagtgcg ccctcagaag cgccagcggc gtgactcgga
     9961 ttcctcgctc gactggatcc accagtctcc ggtgctcact gcgatttcca gtccgatgag
    10021 ccgttgtacc gtggcgcgaa agcggtcgcg gtagaacagc aggaagatcg tgtccccgcc
    10081 gcgactgacg aactctgaca gcacggccgc cagcagatcg agtttctgac tcaggcccgc
    10141 gatgctgccc gcgaccgcct tgcgataggc gctcttgttc tcggccggcg gtggcatcgg
    10201 ccggccgact tccgaggccg cgcgcgcgtc caccagctcg agcacgccgg acacggtcga
    10261 gggtttttcc agcacgctga gctgttgcgc ggtgagcgcg acggtttcaa cgcgcgcggc
    10321 gaggatcgtt tcgacaatcg tcgccagatc gcgcgcttcg cgcagcaatt catccagcgt
    10381 ggtctgattc tgatgccact cgagcgtgcg tcgctgctgc tcgaccgtca ccagcacacc
    10441 gaccagtgcc agcatgccga acacaccgcc gacgaactca ccaaagcgcg cccagtcctg
    10501 ggtgtgagcg gagggcagcc aggccgtatt cgggccgaag gtccaggcat acagcccgat
    10561 ggccgccgcg gcagccagtc cgagcacgat cagccatgtt ctacccatgc ccttatcgta
    10621 gcggatctgc aaaaatggat gctgtgaagt ccctggggaa aaaagcacaa ggggcgcgcc
    10681 ttgaacgaat gcaggcctcg ccgcgctggc tgggcgagcg tttcgccaac ctgcatccca
    10741 ttgcgcccgg actgcgcgat ccgaacacac cgatgcccac gctcggcgaa ttcatctgcg
    10801 gcggcgggcg gcgcgtgccg gcagcgccgc taccggcgct gaacccgctg gtgtggtgga
    10861 aacgcccgcc ggacagcgga ctgcgtgcca cctggctggg tcattccacg ctcttgctgg
    10921 agatgggtgg ctatcgtgtg ctgaccgatc cggtctgggg tccgcgcgcc tcgccgtcgc
    10981 gactgctcgg tccgaagcgc ttcagccgat tccggtacgt ttgcgcgcca tgccgcagct
    11041 cgatctggtc gtgatctcac acgatcacta cgatcatctc gactatccga ccattcgaga
    11101 actggccagg cacccggtgc cgttcgtcac ctcgctcggc gttggcgcgc acctcgaggc
    11161 ctggggcgtg ccgcccgaac gaatcgtcga gctcgactgg tgggagaccc acaccgtgcc
    11221 gaacacgggt ctgtcggtca cggcggctcc atcgcagcat ttctccggtc gcggtctgaa
    11281 ggatcgcaac gccacgctct ggtcctcgat ggtgatccgc tcgccgcggc acgctgtgtt
    11341 cttcagcggt gataccggcc tgaccagcga gtaccgctcg atcggcgagc ggctcggacc
    11401 cttcgatctg gtgatgctcg aggtcggcgc gttccatccg gcatggggcg acatccatct
    11461 cggacccgag aacgcgctgc gtgcgctgaa gttcctgggc agcggcacct tcctgccgat
    11521 ccactggggc accttcgcgc tggcgatgca tgcctgggat cagcccgccg aagtgatgtt
    11581 cgatctggcg ccgaaggcgg acgtgcgact gctcatgccg cgactgggcg aaccggtgga
    11641 gccggtccag gtgggcgctg tcacaccctg gtggcgtggc gtggatacga tgcaggcctc
    11701 ggtgccgcac ccggatgagc cgatgccgat gccgataccg aagacgatgt cctggccgat
    11761 cgactagcct caccgcccgc agggctcaca ggggagggct gaatgaaatt ccgtctcgcc
    11821 atgctgtgct tgctggcacc gctggcactc attgcgctgc tgcgcgcggg cagcgccttg
    11881 gctgatgacg cgaagagact tcgcctgatc acctggtccg actatgcgcc cgccgaggtg
    11941 atcgcgcagt tcaaacagga gagcggcatc caggtcgagg tgacgctctc caacaacgag
    12001 gagatcatct ccaagctgcg cgccaccggc ggcgccggct atgacctggc gcagccctcg
    12061 caggatcgca tcaccggcgt gcagcgtgac ttcggcgtct acaagccgat cgacctgtcg
    12121 caggtgaagc tcgatcagtt tcgttccgag ctgctggaca tcacgcgcaa aaacgcgacg
    12181 ctggacggca agctctatgc gctaccctac gtgtggggca ccgatgatct ggtggtcaac
    12241 accagggcag cgaagctggc tgactacacc gatctgtgcc gtgcagatct caagggcaag
    12301 agcagcgtgc gtctgcggcg cccgaccctg atggcctttg cgttcgcctc cggcaaggac
    12361 ccgttcgcgc tctacgcgga tagcaaagcc tatgcggcgc tgatggacag cgtcgggcag
    12421 acgctgtccg cctgcaaggc gaacgtgcgc ttcttctacg acaacaagga tcaattgctc
    12481 aacgcgatgc gctcgggcga agtcgccgcc gccatggcct gggacaccga gggctggcgg
    12541 ctgaaccgcg agaatgccga catcaaagtg atcgtgccgc gctccggggc actcggatgg
    12601 atggacacct tcgcgctgcc ggcccgcggc cgcaacgacg ccggcgcgta cgcctggatc
    12661 aatttcacga tgcgcccgga catcgccgcg cgcatcacca aatcggtcgg caactggact
    12721 gccgccacag gcaccgagac gctggtggat gcgacgctgc gtgcgcagta caacgccacc
    12781 ttcccggaag gtgcgttcaa gaacatccac tggtatccgg cgatcccctc cgggctggag
    12841 gagatcgagg gccgggtgct ggaccggatc aaggctgccc gttgagcacg cccgatctgg
    12901 cggtggatgc ggtggcgaag cacttcgccg ggcatcgcgc cgtcgacgcg ctgtcgttcg
    12961 acgtcgcgcg cg
//
LOCUS       DATMRM010000319         7642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_511515, whole genome shotgun sequence.
ACCESSION   DATMRM010000319 DATMRM010000000
VERSION     DATMRM010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7642
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511515"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VNZ06_09525"
     CDS             <1..236
                     /locus_tag="VNZ06_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HWW21031.1"
                     /translation="LSTIAEVQARGGPVVAITQPGQNKLDVEAVFEVPQSLPEIDPIL
                     LNLPLQLLAYHVAMRRGRDVDQPRNLAKSVTVE"
     gene            422..991
                     /locus_tag="VNZ06_09530"
     CDS             422..991
                     /locus_tag="VNZ06_09530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWW21032.1"
                     /translation="MPTHAKDTGRSSEFGQFMQSYQDMVYSTAMRIVGNDAQAQDIAQ
                     DVFLKAFEHFDRLRDSPTIGGWLKTVTTNHCLNHLSRYRKRWRFFSEFQRPGEQDDEP
                     EIDFAVPDTLFDDLAADERHDIVERALGRLPEHQRVALVLYHFEELAYDEIARTLRVS
                     LAKVKTDILRGRVNLAKYLADARPSNEPN"
     gene            1000..1491
                     /locus_tag="VNZ06_09535"
     CDS             1000..1491
                     /locus_tag="VNZ06_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21033.1"
                     /translation="MKLNTEDQARLEKLIAAGLNRQPALKAPASLQSRVLAEIARRAA
                     LPWYHRSFRHWPMVMQVSFVLTALATVVLARSVILWSHPVHAASELAAPVSGSFSFLE
                     SLGSAWSAMASMFGAIANALLHRVPGFWLVVGIGVAFAMYAMLAGIGVAVYRTLDGGR
                     THA"
     gene            1848..3338
                     /locus_tag="VNZ06_09540"
     CDS             1848..3338
                     /locus_tag="VNZ06_09540"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWW21034.1"
                     /translation="MNVNVDSDDNSVFNFGGNSNLAAGDQADSVVSIMGSSTSAGDVS
                     QSVVSVLGNSHVTGPVGDSVVAVLGNAYVDSKVEHDVVAVLGNVTLGPNADIGGDIVA
                     VGGTVHQDPAAQVHGSVQNVGIGQHFTRGRNWVEHCLMMGRPLAFVSGIGWAWTLAFV
                     ALALYLFASLLFAPAVEHCIHVLEKYPGQSLLTMILTMIATPIVFVILFITVIGIVAV
                     PFLALALFLMKFFGKLVILAWIGRRLLRSHQPITAMDTVSAVLVGGLLVMLMYCVPVL
                     GFILYNLLGLVGLGVVIYALLLQIQGRRTTATAGTAPGGASPPPGATAAQMSAVLNGE
                     PEAAAAAGGAAATASDTQGAHVPPPLPAPGSARSGRVSGEAAIALPRAGFWIRMLALL
                     LDAVLVGVVMHLIGASSDMFLIVLAAYGVVMWRLRGSTVGGIIFNLAVVKHDGHEMDW
                     GTAVVRALSAFLSLFALGLGFIWIAIDSENRSWHDKIAGTVVVRVP"
     gene            complement(3354..3770)
                     /locus_tag="VNZ06_09545"
     CDS             complement(3354..3770)
                     /locus_tag="VNZ06_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21035.1"
                     /translation="MNGLPTGRVIRRMLGMGALLTCAGTAFSAEPAGAVAPAHGPMLM
                     IYVSQPLMSGASRVYGLRLDQVAQIVNAPITANGFYAASPQRSLVDLQIRRRADVRLE
                     LGNRVTWNLRRREFEFPDMRPTKPIDFVAQAHQNRP"
     gene            complement(3934..4308)
                     /locus_tag="VNZ06_09550"
     CDS             complement(3934..4308)
                     /locus_tag="VNZ06_09550"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HWW21036.1"
                     /translation="MSPPDAAPAAVPAAAPAAAPVFGEPWQAQAFALTLELSRRGHFS
                     WSQWTQALAEELQRARTRGELNDGSHYYHHWVAALERLVLTSNLCDASTLQQRKLAWI
                     HAYEHTAHGRPVELARNTPDLP"
     gene            complement(4295..4954)
                     /gene="nthB"
                     /locus_tag="VNZ06_09555"
     CDS             complement(4295..4954)
                     /gene="nthB"
                     /locus_tag="VNZ06_09555"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR03888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HWW21037.1"
                     /translation="MNGVHDMGGMHGLGPLEHPPNEPVFHAPWEARVFALRRAMGAWR
                     KWNIDASRHAIEKLPASQYLSLDYFQRQFEAFLQMLVAAGFITPQEIVDGQARGAQER
                     LTPAMNVDGAAALIRKGVPCRREAAVQAHFEVGQAVHTRNLQPSGHTRLPRYARDKVG
                     TIDRCHGVFVYPDSNAHFLGEKPQPLYSVRFEARELWGPQASATDSVYLDLWEDYLEP
                     A"
     gene            complement(4951..5568)
                     /gene="nthA"
                     /locus_tag="VNZ06_09560"
     CDS             complement(4951..5568)
                     /gene="nthA"
                     /locus_tag="VNZ06_09560"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR01323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HWW21038.1"
                     /translation="MSKLPDHAQDDAPGHSDVPSDIALRVKSLESLLVDKGLVDPAAL
                     DFLIDSYEQRIGPRNGARVVARAWTDPSYRQRLLSNADSAIAELGYAGMQGEHMVVVE
                     NTPRTHHLVVCTLCSCYPWPVLGLPPVWYKSHAYRSRAVIDPRGVLREFGVSLPEDVE
                     VRVWDSTAEIRYLVLPQRPPNTQSLDESALAGLVTRDAMIGVSAL"
     gene            complement(5565..5795)
                     /locus_tag="VNZ06_09565"
     CDS             complement(5565..5795)
                     /locus_tag="VNZ06_09565"
                     /inference="COORDINATES: protein motif:HMM:NF018500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HWW21039.1"
                     /translation="MPRSSTNGLPIPHRELSADALRGVVEAFVLREGTDYGMREFTLE
                     EKVSHVMAQLDRQEAVILFDPDTRSIHIVVVQ"
     gene            5856..>7642
                     /locus_tag="VNZ06_09570"
     CDS             5856..>7642
                     /locus_tag="VNZ06_09570"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HWW21040.1"
                     /translation="MYALVAAIVVMVLSGIIGWRAAQSSVAASQAIARNKDAQLNLDR
                     TLSAVRDAESGQRGYLLTGRDTYLQPYNAAVADLPSRLSQLRAGVSGDPTAVQQVDRL
                     QDTIAAKMEELARTIQLQQSRGRANAMAEVLTDRGAQLMDAVLEQVAVLQTQQSKLFE
                     SEIQQLSAARVRVAISVFAVRFLTIALLITMVLVVRRETRQLRLSEARLAITLRSIGD
                     AVIATDSQGRVVLLNTVAEELTGWTSARARNAPLDRVFRLVSESHREPVESPVARVMR
                     EGRVVGVGNHTLLLRRGGREVPIEDSAAPIRDDSGAIIGVVLVFRDATSERQLARQLL
                     EADQKKDEFIAVLAHELRNPLAPILQAVQISRRPGATPQQLSWSLDVIARQGRAMGHL
                     LDDLLDVSRITRGTVEVNKSRVLLSAVIDGAVETARPLIQDRQHSLVLDVPPQTVMIE
                     VDPLRISQVIANLLTNAAKYTNPGGVLQLKASIDESLLTLCVTDNGIGIDPKALPRVF
                     DMFVQVKGPLDRQEGGLGIGLAVSKKLVELHGGSVEARSEGPNRGSEFIVRMPCVVET
                     TEMLPPAPVSPAATKRGRLRVLVADDNRDA"
BASE COUNT         1362 a         2365 c         2484 g         1431 t
ORIGIN      
        1 acctgtccac catcgccgag gtacaggctc gtggtgggcc ggtggtggcc attacccaac
       61 ccggccagaa taagctggat gtcgaggccg tgtttgaggt gccgcagtcg ctgccggaaa
      121 ttgaccccat tctgctcaac ctgccgcttc agctgctggc ctatcacgtg gcgatgcgtc
      181 ggggccggga tgtcgaccag ccgcgcaacc tcgcgaagtc cgtcacagtg gagtagcgct
      241 agggggtgac ccacaatagg ggatctgggg atcgtattgt cgatataatt atcttaaata
      301 taaataactt atgacatttt attgagctca tttctgtcat cttgccggcc gtcccgaatc
      361 tgtaatcacc ctgcaacttt ggctgcgttg cggtgttctg tgcttaaacg ctgaaactca
      421 agtgccaaca catgccaagg acacgggtcg gtcgagcgaa ttcggacaat ttatgcagtc
      481 ctatcaggat atggtgtatt caacggcgat gcgcatcgta ggcaacgatg cgcaggcgca
      541 ggacatcgcg caggacgtgt tcctgaaggc attcgaacat tttgaccggc tgcgcgacag
      601 ccctacgatc ggcggctggc tgaagacggt gaccaccaat cactgcctga accatctgtc
      661 gcgctaccgc aagcgatggc gcttcttctc agagttccag cggccgggcg aacaggacga
      721 cgagccggag attgacttcg cggtaccgga tacgttgttt gacgatctgg ccgccgacga
      781 gcggcacgac atcgtcgagc gcgcgttggg tcgactgccc gaacatcagc gtgtggcgct
      841 ggtgttgtat cacttcgagg aactggcgta cgacgagatt gcgcgcacgc tgcgcgtctc
      901 gctcgccaaa gtgaagaccg acatccttcg cggacgcgtg aatcttgcca agtacctggc
      961 cgatgccagg cccagcaacg aacccaattg atgactgcca tgaaattgaa cactgaagat
     1021 caggctcgtc tggaaaaact catcgccgcg ggactgaacc ggcaaccggc gctcaaagca
     1081 cccgcctcat tgcagtcgcg cgtgctcgcc gagatcgcgc gccgcgcggc actgccctgg
     1141 tatcaccgct cgttccgtca ctggccgatg gtgatgcagg tcagttttgt gctgaccgcg
     1201 ctcgccaccg tggtgctggc acgcagtgtg atcctctggt cgcacccggt gcatgccgcc
     1261 agcgagctgg cagcaccggt cagcggcagc ttctcgttcc tggagagcct cggctcggcg
     1321 tggagtgcga tggcctcgat gttcggcgcg attgccaacg cgctgctgca tcgggttccc
     1381 ggattctggt tggtcgttgg tatcggcgtg gcattcgcga tgtacgcgat gctcgccggc
     1441 atcggcgtgg cggtctaccg aacactcgac ggcggcagga cgcacgccta actacacagt
     1501 tggtccatat gaatattcaa tcccgaaaat tgcaccggta cgcttcactg ctaggtgctt
     1561 tgctgctggt tggcatgatg ccggccacgt acgcgacggc aaccgcgaag agcagcgctc
     1621 cgcccgcgca tcccaacgcg ccagcggcca cggcaccagc ggccaccgca ccagcggcca
     1681 ccgcaccggc acagccctcc accacaccgc cggtggctcc gacgccgccc cagcaagatc
     1741 agtcggatca gtctgtccaa tctgaccagt cagatcagtc tgacagcggg tcgaattcct
     1801 ccgaaacgtt ccccaagcat ggccactggc atcgccatcg caatcacgtg aatgtcaacg
     1861 tggacagcga cgacaactcg gtcttcaact tcggaggcaa ttcgaatctc gccgcgggcg
     1921 atcaggccga tagcgtggtc tcgatcatgg gttcctccac cagcgccggc gacgtgagtc
     1981 agtccgtcgt ctcggtgctg ggcaattcgc acgtcaccgg tccggtcggg gacagtgtgg
     2041 tcgcggtgct cggcaacgct tatgtcgaca gcaaggtcga gcacgacgtg gtcgcagtgc
     2101 tcggcaacgt gacactcggt cccaatgctg acatcggcgg ggatatcgtc gcagtcggtg
     2161 gtacggtaca tcaggatccg gcggcgcagg tgcacggcag cgtacagaac gtcgggatcg
     2221 gacagcattt tacccgcgga cgcaactggg tcgagcactg cctgatgatg ggccggccgc
     2281 tggccttcgt atccggtatc ggctgggcct ggacgttggc gttcgtcgca ttggcgttgt
     2341 acctgttcgc ctcgctcctg ttcgccccgg cagtggagca ctgcattcat gtgctcgaaa
     2401 agtatcccgg acaatcgttg ctgacgatga tcctgacgat gatcgcaacg ccgatcgtct
     2461 ttgtgatcct gttcatcacg gtgatcggaa tcgtcgcggt gccgttcctg gcgctcgcgc
     2521 tgtttctcat gaagtttttc ggcaagctgg tcattctggc gtggataggc cgtcgcctgc
     2581 tgcgctcgca ccaaccgatc acggcgatgg atacggtgtc tgcggtactg gtgggcggat
     2641 tgctggtgat gctgatgtac tgcgtgccag tgctcggctt catcctctac aacctgctcg
     2701 ggctcgtggg tctgggcgtg gtgatctatg cgttgctgct gcagatccag gggcgccgta
     2761 cgactgccac tgcgggcaca gcgccgggtg gtgcatcgcc gccgccggga gccaccgccg
     2821 cacagatgtc cgcggtcctg aatggcgagc ccgaggccgc cgccgcagcc ggcggtgctg
     2881 cggccacagc cagcgacacg cagggcgcgc acgtgccgcc tccgctgcct gcgccaggca
     2941 gcgccaggtc aggcagagtg agcggcgagg cggcgatcgc gctgccgcgc gcgggtttct
     3001 ggatccgtat gctggcgttg ctgctggacg cggttctggt aggcgtcgtg atgcatctaa
     3061 tcggcgcttc gagcgacatg tttctgatcg tgctcgccgc ctacggtgtc gtgatgtggc
     3121 ggctgcgtgg cagcaccgtc ggcgggatca ttttcaatct ggcggtggtc aagcacgatg
     3181 gacatgagat ggattgggga accgcggtcg tgcgcgcact gagtgcgttc ctgtcactgt
     3241 tcgcgctcgg gctgggattc atctggatcg cgatcgattc cgagaaccgc tcctggcacg
     3301 acaagatcgc cggtaccgtc gtcgttcggg tgccgtagcg catcggctaa agatcagggc
     3361 cgattctggt gagcctgcgc gacgaagtcg atcggcttgg tcgggcgcat atcgggaaat
     3421 tcgaattcgc gccgtcgcag gttccaggtt acgcgattcc ccaactccaa ccgcacgtcg
     3481 gcccgacggc ggatctgcag gtccaccagc gaacgctgcg gtgaggcggc gtagaagcca
     3541 ttggcagtga tcggtgcgtt gacgatctgg gcaacctgat cgaggcgcag accatagacc
     3601 cggctggcgc cgctcatcag cggctggctc acatagatca tcagcatcgg gccgtgtgcg
     3661 ggggcgaccg cgcccgccgg ttcggccgag aacgctgtgc cggcacaggt cagcagggca
     3721 cccattccca gcatccgtct gatcacccgt cccgtcggaa gcccgttcat gccaagctcc
     3781 tccagatccg cacgatccgc agtcagtatg ctcgccgcga catgaacggc agcccacaat
     3841 gaccataagg ggcggtcagc ggccggttct gtgaccgaaa cgccggatta tgcaaacaag
     3901 ctgtctggtg gatgcaacgc tgaaagcacc cgcctacggt aggtcgggtg tattgcgcgc
     3961 gagttcgacc ggccggccat gcgccgtatg ttcataggca tggatccagg cgagcttgcg
     4021 ttgctgcagc gtgctcgcat cgcacagatt cgaagtcaat accaggcgct cgagggccgc
     4081 gacccaatga tggtagtagt gcgagccatc gttcagctcg ccgcgcgttc gcgcgcgctg
     4141 cagttcctcg gccagcgcct gcgtccactg cgaccagctg aagtgaccac ggcgtgagag
     4201 ctcgagcgtc agtgcgaacg cctgcgcctg ccatggctcg ccgaacactg gtgccgccgc
     4261 cggtgccgcc gccggcaccg ccgccggcgc cgcgtcaggc gggctcaaga tagtcttccc
     4321 acagatccag atacaccgaa tccgtggccg aggcctgcgg gccccacagc tcgcgcgcct
     4381 cgaagcgcac cgagtacagc ggttggggtt tttcacccag aaagtgcgca ttgctgtccg
     4441 gatacacgaa cacgccgtga caacgatcaa tggtgccgac cttgtcgcgc gcgtagcgcg
     4501 gcaggcgcgt gtggccgctg ggctgcagat tgcgggtatg caccgcctgg ccgacctcga
     4561 agtgtgcctg cacggcggct tcgcgacggc agggcacgcc cttcctgatc agggccgcgg
     4621 cgccgtcgac gttcatcgcg ggagtgagcc tttcttgcgc gccgcgcgcc tggccgtcga
     4681 caatttcctg cggtgtaatg aatccggccg cgaccagcat ctgcaggaag gcttcgaatt
     4741 gccgctgaaa atagtccagc gacagatact gtgaggcggg tagcttttcg atggcatgac
     4801 gggaggcatc aatgttccac ttgcgccacg cgcccatggc ccggcgcaga gcgaacacgc
     4861 gcgcttccca cggcgcatga aacaccggct cgttcggcgg gtgttcgagc ggacccaagc
     4921 cgtgcatgcc gcccatgtcg tgtacaccat tcatagcgca ctgactccga tcatggcgtc
     4981 gcgcgtgacc agccccgcca gggccgactc gtcgagtgac tgggtgttcg gcgggcgctg
     5041 cggcaacacg aggtagcgga tttccgcagt gctgtcccag acgcgcacct cgacgtcctc
     5101 gggcaagctg acaccgaatt cgcgcagcac accgcgcggg tcgatcaccg cccgtgagcg
     5161 gtaagcgtgc gacttgtacc agaccggcgg cagtccgagt accggccacg gatagcatga
     5221 acacagcgtg cagaccacca gatggtgcgt gcgcggcgtg ttctccacca cgaccatgtg
     5281 ctcaccctgc atgccggcat agccgagttc cgcgatggcg gaatcggcgt tgctgagcag
     5341 tcgctgccgg taggaggggt cggtccaggc gcgggccacg acgcgtgcgc cattgcgtgg
     5401 gccgatccgc tgctcatagc tatcgattag aaaatccagc gcggccgggt ccaccaggcc
     5461 tttgtccacc agcagggact ccagcgactt cacccgcagc gcgatatcgg acggcacatc
     5521 gctgtgccca ggagcatcgt cctgcgcgtg atcgggcaac ttactcattg gaccaccacg
     5581 atgtggatgc tccgggtatc cgggtcgaac aggattactg cctcctgacg gtcaagctgt
     5641 gccatgacgt ggctcacctt ttcttccaaa gtgaattcac gcataccgta gtcggtacct
     5701 tcgcgcaaca cgaaagcttc caccacaccc cgcagtgcgt ccgcgctcag ctcgcggtgc
     5761 gggatcggaa gtccgttcgt gctcgagcgt ggcatatact gcatcttacg tcggaggacg
     5821 gacttgaagg aatgccgcga tcaagtttga tagtcgtgta tgcactggtc gcggccattg
     5881 tcgtgatggt gctgtccgga atcattggtt ggcgtgccgc gcaatcgtcg gttgccgcga
     5941 gccaggcgat tgcgcgcaac aaggacgcgc agctcaatct ggaccgtacg ctgtcggcgg
     6001 tgcgggatgc ggaaagcgga caacgtggct atctgctgac cgggcgagac acctacctgc
     6061 agccgtacaa cgccgcggtc gctgacctgc cgtcacgact gtcacaattg cgagccggtg
     6121 tttcgggcga tccgaccgcc gtgcagcagg tggatcggct gcaggacacg atcgccgcca
     6181 agatggagga gctcgcgcgg accattcagc tgcagcagag ccgcggccgt gcgaatgcga
     6241 tggccgaggt actgaccgac cgcggtgcgc agctcatgga tgccgtgctg gagcaggtcg
     6301 cggtgctgca gacccagcag tcgaagctgt tcgaatccga gatccagcaa ctgagtgcgg
     6361 cgcgcgtgcg cgtcgcgatc agcgtattcg cggtgcgctt tctgacgatc gcattgctga
     6421 tcacgatggt gctggtcgtg cggcgcgaga cccgtcaact gcggctgagc gaagcgcggc
     6481 tggcgatcac gttgcgcagc atcggtgacg cggtgattgc gaccgattcg cagggacgtg
     6541 tggtgctgct gaatacggta gccgaggaac tgaccggctg gaccagcgcc cgcgcgcgca
     6601 atgcaccgct cgatcgcgtg ttcaggctcg tcagtgagtc gcatcgcgag ccggtcgaaa
     6661 gtccggtggc gcgcgtcatg cgcgaaggac gcgtggtcgg cgtcggcaac cacacactgc
     6721 tgttgcgccg tggcggtcgt gaggtaccga tcgaggacag cgccgctccg atccgcgacg
     6781 actccggcgc gatcattggc gtggtgctgg tatttcgcga cgcgacctcg gagcgccagc
     6841 tcgcgcggca actgctggag gcggatcaga aaaaggacga gttcatcgcg gtgctggcgc
     6901 acgagctgcg caatccgctg gcgccgatcc tgcaagccgt tcagatctcg cgtcggccgg
     6961 gagccacccc gcagcagctg agctggagtc tggatgtcat cgcacgacag ggccgggcga
     7021 tggggcatct gctggatgat ctgctcgacg tgtcgcgcat cacgcgcggc acggtcgagg
     7081 tcaacaagtc gcgtgtgctg ctctcggcgg tgatcgatgg cgcggtcgag accgcacggc
     7141 ctctgatcca ggaccgccag cactcgctgg tgctcgacgt cccgccgcag acggtgatga
     7201 ttgaggtgga tccgctgcgc atctcccagg tgatcgccaa tctgctgacc aacgcagcga
     7261 agtacaccaa tccgggtgga gtgctgcaat tgaaggcgag cattgacgag tcgctgctga
     7321 cgctgtgcgt caccgacaac ggcattggta tcgacccgaa ggcgctgccg cgcgtgtttg
     7381 atatgttcgt gcaggtcaag ggaccactgg accgccagga aggtggcctc ggcatcgggc
     7441 tcgcggtttc gaagaaactg gtggaactgc acggtggttc ggtggaagcg cgcagcgaag
     7501 ggccgaaccg cggcagcgag ttcatcgtac gcatgccatg cgtggtcgag actaccgaga
     7561 tgctcccacc cgcgccggtg agtcctgcgg cgaccaagcg cgggcgactt cgcgtgctag
     7621 tggcggatga caaccgcgat gc
//
LOCUS       DATMRM010000320         7636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3264339, whole genome shotgun sequence.
ACCESSION   DATMRM010000320 DATMRM010000000
VERSION     DATMRM010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3264339"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2324
                     /locus_tag="VNZ06_09575"
     CDS             <1..2324
                     /locus_tag="VNZ06_09575"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004666357.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWW21041.1"
                     /translation="FHYATRAGISFGIDDIVIPEQKTRIVTSADNEVKEIQDQYASGL
                     VTNGERYNKVVDIWSRANDQVAKAMMEKLGSEEATDLKGMRHRQKSFNSIFMMADSGA
                     RGSAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTAL
                     KTANSGYLTRRLVDVAQDLVVTEEDCGTTNGLSMAPLVEGGDVVEGLGERVLGRVTAT
                     DVQKPGTEEVLVEASTLIDEGLVRLIEQEGVDQINVRSPITCRTRYGVCSKCYGRDLA
                     RGRLINIGEAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASSVEIRNKGTLRFHH
                     IKTVEHEKGHLVAVSRSGEIGVVDEFGRERERYKIPYGATINAKEGAKVLGGQIVATW
                     DPHTHPVVTEVAGFIKFQDFVDGMTVATQVDDVTGLSSTVVMDAKQRGGKDLRPTIKL
                     LGPKGKEVTFANTNIPAVYTLPPGALINLEDGAKVSVGDIIARIPQESSKTRDITGGL
                     PRVADLFEARKPKDPAILAEKSGTVSFGKETKGKRRLVITSDDGEKYEELIPKWRQLN
                     VFEGETVERGEVIADGEPNPHDILRLQGVEALANYLVREIQDVYRLQGVKINDKHIEV
                     IIRQMLRKTEVVTSGETTLLRGEQIDRSRALDINDRAETDGKQAASLQPVLLGITKAS
                     LATESFISAASFQETTRVLTEAAVRGLKDDLRGLKENVIVGRLIPAGTGFAAHAHRRR
                     KLDESGRRSFMDVGGAVGDMDDVGVGEEESAAG"
     gene            2697..3362
                     /locus_tag="VNZ06_09580"
     CDS             2697..3362
                     /locus_tag="VNZ06_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21042.1"
                     /translation="MEYSELHIARAVHSDQQDEAQLATRLLENVTLYSSWEQSHGRFM
                     RDVAAAARPAVQLVELRKVSFSTLHRKAPFEYLRDRHITGPARRVLIQRLFGTQHYGR
                     TLVREHAAYISSACSYMCADSLCGEVLGDQVFCEALSSYEEAYAEYYRAYCDSLLVEA
                     AGQEATPLQSLLPYLRYQLKVIREHLLAGAPQKSDFKMLQSLYAKSGDTQKLPVLDLN
                     LAL"
     gene            complement(3366..4454)
                     /locus_tag="VNZ06_09585"
     CDS             complement(3366..4454)
                     /locus_tag="VNZ06_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWW21043.1"
                     /translation="MGRHRGGRGFQGFHPGGFGGDGGFGHRGRIRGRKIGSDDLQLLL
                     VLLLEERPRHGYELIKALEQRSSGFYSPSPGMIYPALTYLEELGYTTVSSEAGRKLYE
                     LAPAGRAYLNQHRAAAELVLAQLEQFGRRLFAGPEAAEPTAPSASPHHAPRRPLSLGV
                     NTDAVLARLRDEAERTRPPDGGPRHSGIHDPFQYAEYGFSISQEQGELIYLLCRQMRA
                     RRVIDFATSVGVSAIYFGAAMRDNQDGLVIGSEIVPQKVRTAEQNLNQAGVDFWVNIR
                     LGDARETLRDLDEPYDFALIDGWPGDGTRSLARDVVEIVAPTIRRGGMLMNDNGEPDY
                     LEYVRDPRNGFISITLPMKGGTELSLKR"
     gene            4631..6565
                     /locus_tag="VNZ06_09590"
     CDS             4631..6565
                     /locus_tag="VNZ06_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWW21044.1"
                     /translation="MLNFSDLTLRRGKRVLFSGASFNLFRGEKIGITGENGSGKSSLL
                     ALVRGELTPEAGSFEMPRQLALAHVSQELLPSERPAIDFVLDGDPQLRELELRLQEAE
                     TAEDGEHLAELHARYGEIGGYDAVSRAGQLMHGLGFAASDEQRFVSAFSGGWRVRLNV
                     AQALMCRSDLLLLDEPTNHLDLDAIVWLEGWLREYPGTLLLISHDREFLDRVTNRIVN
                     LDHGHARAYRGNYSAFEQQRASELAEQAALYARQQREIRHTQSFVERFRAKASKARQA
                     QSRLKALERMQRIAPAHVDSMFEFSFAPPTKLPRPLLALERQSVGYAARTVVSGISLS
                     IAPGDRVALLGRNGAGKSTVMKLLAGALSASVGTRTEARDLKIGYFAQHQLEQLNAKD
                     TPCQHLRELSESMADQATEQQLRDFLGAFGFQGDRVFEPIAPFSGGEKARLVLALLAY
                     QRPNLLLLDEPTNHLDLEMRQALAVALQDYEGAVMLVSHDRHLLRVVADRLLLVDQGR
                     LSEFDGDLEDYARRLSTNSPAPAPLTATAPAVAETPRESAEARKARKRAQAEFRSALA
                     PLRARIAELERELNRLLAERGRIEQQLANPRSDADRARLVQLAQARASIAKRVSEVES
                     TWLQASEQLEARIAEQQDVD"
     gene            6725..7099
                     /gene="rpsL"
                     /locus_tag="VNZ06_09595"
     CDS             6725..7099
                     /gene="rpsL"
                     /locus_tag="VNZ06_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HWW21045.1"
                     /translation="MATTGQLIRQPRASRAEKTKVPALGASPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGYEVSSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGTL
                     DCAGVGNRKQGRSKYGAKRPKK"
     gene            7116..7595
                     /gene="rpsG"
                     /locus_tag="VNZ06_09600"
     CDS             7116..7595
                     /gene="rpsG"
                     /locus_tag="VNZ06_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003369032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HWW21046.1"
                     /translation="MSRRAAAPTRTILPDPKFNSDMLAKFMNVVMHSGKKSAAEHILY
                     GAIERIGEKTGRQGSEAIELLSQALENVKPVVEVKSRRVGGATYQVPVEVRASRRQTL
                     AMRWVIEAARARSEKSMAFRLAHELLDAAENRGAAVRKREDTHRMAEANRAFAHYRW"
BASE COUNT         1476 a         2330 c         2486 g         1344 t
ORIGIN      
        1 gcttccacta cgcgacgcgc gccggaatct ccttcgggat cgacgacatc gtcattccgg
       61 agcagaagac ccgcatcgtc acctccgccg acaacgaggt gaaggaaatc caggaccagt
      121 acgcctcggg cctggtcacc aacggcgagc gctacaacaa ggtggtcgac atctggtcgc
      181 gggcgaacga ccaggtggcg aaggccatga tggagaagct cggctccgag gaggccaccg
      241 acctcaaggg tatgcgccac cggcagaagt cgttcaactc gatcttcatg atggccgact
      301 cgggcgcgcg tggttctgcg gcgcagatcc gacagctggc cggcatgcgc ggactgatgg
      361 ccaagcccga cggctcgatc atcgagacgc cgatcacggc gaacttccgc gaaggtctga
      421 acgtattgca gtacttcatc tcgacgcacg gcgcccgcaa gggtctggcc gacaccgcat
      481 tgaagacggc gaactcgggc tacctgaccc gtcgcctggt cgacgtggcg caggacctgg
      541 tggtcaccga agaggactgc ggaaccacga atggtctgtc gatggcgccg ctggttgagg
      601 gtggcgatgt ggtcgaaggc cttggcgagc gcgtactcgg ccgtgtgacc gcgaccgatg
      661 tgcagaagcc gggtaccgag gaagtgctgg tggaggcaag cacactcatc gatgaaggcc
      721 tggtgcgcct gatcgagcag gaaggcgtcg atcagatcaa cgtgcgttca ccgatcacct
      781 gccgtacgcg ttacggcgta tgttccaagt gctacggacg cgatctggcg cgtggccggc
      841 tcatcaatat cggcgaggcg gtgggcgtca tcgccgcaca gtcgatcggc gagccgggaa
      901 cgcagctgac gatgcgtacc tttcacatcg gtggtgccgc atcgcgcgcc gcggcggcca
      961 gcagtgtgga aatccgtaac aagggcacgc tgcggtttca tcacatcaag accgtcgagc
     1021 acgagaaggg gcacctggtc gcggtctcgc gttcgggcga gatcggcgtg gtcgatgagt
     1081 tcggtcggga gcgcgagcgc tacaagattc cgtacggcgc gaccatcaat gcgaaggaag
     1141 gcgcgaaggt actgggcggc cagattgtgg ccacctggga tccgcatacg cacccggtcg
     1201 tcaccgaggt cgcgggcttc atcaagttcc aggacttcgt cgatggcatg acggtggcga
     1261 cccaggtgga cgacgtgacc ggcctttcct ccacagtggt catggacgcc aagcagcgcg
     1321 gtggcaagga tctgcgtccg accatcaaac tgttgggccc gaagggcaag gaagtgacct
     1381 tcgccaacac caatatcccg gcggtgtaca cattgccacc gggcgcgctc atcaacctgg
     1441 aggacggcgc caaggtgtcg gtcggggaca tcatcgcccg cattcctcag gaatcatcga
     1501 agacgcgcga catcaccggc ggtctaccgc gggtagcgga tctgtttgaa gctcgtaagc
     1561 cgaaggatcc ggcgatcctg gcggagaaga gcggcacggt cagtttcggc aaggagacca
     1621 agggcaagcg tcgcctcgtg atcaccagcg atgatggcga gaagtacgag gagctgattc
     1681 cgaagtggcg ccagctgaac gtgttcgaag gtgaaaccgt cgagcgcggt gaggtgatcg
     1741 cggacggtga acccaatccg cacgacattc tgcgactgca aggcgtcgag gcactggcga
     1801 actatctggt gcgcgagatc caggacgtct accggttgca gggcgtgaag atcaatgaca
     1861 agcacatcga agtgatcatt cgccagatgc tgcgcaagac cgaagtcgtc acctccggtg
     1921 agaccacgct gctgcgcggc gagcagatcg atcgctcgcg cgctctcgac atcaatgatc
     1981 gggccgagac cgatggcaag caggcggcga gcctgcagcc cgtgctgctg ggcatcacca
     2041 aggcatcact ggcgacggaa tccttcatct ctgcggcttc gttccaggag accactcgcg
     2101 tgctgaccga agccgcggta cgcggactga aggacgatct gcgcggcctg aaggagaatg
     2161 tcatcgtcgg tcgcctgatt ccggcgggca ccggctttgc ggcccatgca catcgtcggc
     2221 gcaagctgga cgagagcggc cggcgcagct tcatggatgt cggcggtgcc gttggcgaca
     2281 tggatgacgt cggtgtgggc gaagaggaaa gcgctgccgg ctagtgccgc aagctcgttg
     2341 caatttagct gcaagccggc gccgcgtagt gtcacatcgc tacgcggcgc cgccgcgtta
     2401 tgtctacccg atccggggcg gtaccagacg ccagcgggtg tcagtcctac aagaattcta
     2461 tgtcctacaa gcgcttgccg gcggcgcgca acggcgccat gtcgccaggt gctaacatat
     2521 tggcagcgcc ggaactgtcg actgatcatt gacaacgcgc gcccggtact ctatttcaga
     2581 agagtgggat gagcgccaac agctcacggg aaacatactt aactacatcc atgccaccga
     2641 tcgccaggac aggtagtcag cgaccagcac accgacccct gagtgtcggg cgactgatgg
     2701 agtattcgga gctgcatatc gcacgtgcgg tccactccga tcaacaggac gaggcgcagc
     2761 tcgcgacccg cttgctcgag aacgttacgc tctacagcag ctgggagcag tcgcatgggc
     2821 gcttcatgcg cgacgttgct gccgctgcgc gtcccgcggt gcagttggtg gaactgcgca
     2881 aggtgtcgtt cagcacgctg catcgtaaag ctccgttcga atatctgcgc gaccggcata
     2941 tcaccggccc ggcgcgtcgt gtgctgattc agcggctgtt cggcacgcag cactacgggc
     3001 ggacgctggt gcgcgagcat gcggcgtaca tcagctcggc ttgcagctac atgtgcgcgg
     3061 attcgctgtg tggcgaggtg ctcggtgatc aggtcttctg cgaggccctc agcagctatg
     3121 aagaggccta cgccgagtac tatcgggcct attgcgattc tctgctggtc gaggcggctg
     3181 gccaggaggc cacaccgctg cagtcattgc tgccgtatct gcgctatcag ctcaaggtga
     3241 ttcgcgaaca cctgctggca ggtgcgccgc agaagagtga cttcaagatg ctgcagtcgc
     3301 tgtacgccaa gagcggcgac acacagaaac tgccggtgct cgatctcaat ctcgcactct
     3361 aggattcatc gcttcagcga cagctcggtt ccgcctttca tcggcagcgt gattgaaatg
     3421 aagccgttgc gcgggtcgcg cacatactcc agatagtcgg gttcgccgtt atcgttcatc
     3481 agcattccgc cgcgccggat agtgggagcc actatctcga cgacgtcccg cgcaagtgag
     3541 cgtgtgccgt cgcccggcca gccatcaatc aatgcgaagt catacggctc gtccagatcc
     3601 cgcagtgttt cgcgtgcatc gccgagtcgg atattgaccc agaaatccac acccgcttgg
     3661 ttcagattct gctccgcggt gcgcactttc tgtggaacga tctccgatcc gatcaccagg
     3721 ccgtcctggt tgtcgcgcat cgcggccccg aaatagatcg cggagactcc aactgaagtg
     3781 gcgaagtcga tgacgcgtcg cgctcgcatc tgtcggcaca acaggtagat gagttcgccc
     3841 tgttcctgag agatcgagaa accgtactcg gcgtattgga aggggtcgtg aatgccgctg
     3901 tgacgcggac cgccatcggg cggccgggtg cgctcggctt catcgcgcaa ccgcgccagc
     3961 accgcgtcag tgttgacgcc cagtgacagc ggcctgcgcg gtgcatggtg tggcgaggca
     4021 gaaggcgccg tgggctcggc cgcctcgggg ccagcgaaca ggcgtctgcc gaactgttcg
     4081 agctgggcca gcaccagctc ggcggccgcc cgatgctggt tgagataggc gcgaccggcg
     4141 ggcgccagct catagagctt gcggcctgcc tcggagctca ccgtggtgta gcccagctct
     4201 tcgagatacg tcagggccgg atagatcatg cccgggctcg gcgagtagaa gccggaggag
     4261 cgctgctcaa gcgccttgat cagttcgtat ccatgtcgag ggcgttcctc gagcagcagc
     4321 acgagcagca attgcaggtc atccgaaccg atctttcggc cgcggattct gccgcggtgg
     4381 ccaaagccgc cgtccccacc gaatccgccc ggatggaaac cctgaaagcc acggccgccg
     4441 cgatggcggc ccatgagtcc caaagaagct cgcataaggg gtgtcgtgtt ccgttttagt
     4501 cgacagtttt taaaatatat cttaagacat atttaaagat atatcttaat tctagtcaat
     4561 cgtttcttta agcgccaccc accgccttgc tgggcacaag cgtcgtctat acgctgccgc
     4621 gcctgccacc atgctgaact tctccgatct caccctgcgc cgaggcaagc gcgtgctgtt
     4681 ctccggcgcg agcttcaatc tgtttcgggg cgagaagatc gggattaccg gcgagaacgg
     4741 aagcggcaag tcgagcctgc tcgcgctggt gcgcggcgag ctcacgccgg aggcgggaag
     4801 ctttgagatg ccccggcagc tcgctctggc ccacgtgtcg caggagctgc tgccgagcga
     4861 gcggccagcc atcgatttcg tgctggacgg cgatccgcaa ctgcgcgagc tggaattacg
     4921 attgcaggag gcggaaaccg cagaggacgg cgaacatctg gcagagctgc atgctcgcta
     4981 cggggagatt ggtggctacg atgccgtcag tcgcgccggc caattgatgc acggactggg
     5041 cttcgccgcg tcggatgaac agcgcttcgt cagcgccttt tcgggtggat ggcgggtccg
     5101 tctgaatgtc gcgcaggccc tgatgtgtcg ttcggatctg ctgttgctgg atgagcccac
     5161 caaccacctg gacctggatg ccatcgtctg gctcgagggc tggctgcgcg aatacccagg
     5221 cacgctgctc ctgatttcgc acgatcggga attcctcgat cgggtgacca atcgcatcgt
     5281 caatctcgat catgggcacg cgcgcgccta tcgcggcaac tactcggcct tcgagcagca
     5341 gcgagcgagc gagctggccg agcaggcggc actgtatgcg cgccagcaac gcgagattcg
     5401 gcatacgcag tccttcgtcg aacgattccg ggccaaggcg tcgaaggccc gccaggcgca
     5461 gagccgactg aaggccctcg agcgcatgca gcgcatcgcc cccgcacacg tggactcgat
     5521 gttcgagttc tcgttcgcgc cgccgacgaa gctgccccgc ccgctgctgg cactcgagcg
     5581 ccagtcggtc ggctacgccg cccgcaccgt ggtgtcgggc atcagtctga gcatcgcacc
     5641 cggcgatcgc gtggcgctgt tgggccgcaa cggcgccggc aagtccaccg tcatgaaact
     5701 gctggcgggg gccttaagtg catcggtggg tacgcgcacc gaggcgcgtg acttgaaaat
     5761 aggttatttc gcccagcatc agctcgagca gctcaatgcg aaagacacgc cctgccagca
     5821 tctgcgcgag ctcagcgagt cgatggcgga tcaagccacc gagcagcagc tgcgcgactt
     5881 cctgggcgcg tttggctttc agggtgaccg cgtatttgag ccgatcgcgc cgttttcagg
     5941 aggggagaaa gcccgattgg tgctggcgct gctggcctat cagcggccca acctgctgct
     6001 actggatgag ccgaccaacc acctggatct ggagatgcgc caggcgctcg ccgtggcgct
     6061 gcaggattac gaaggggcgg tcatgctggt gtcccatgat cgacacctgc tgcgggtagt
     6121 cgccgatcgg ctgctgctgg tcgatcaggg gcggctcagc gagtttgacg gcgatctgga
     6181 ggactacgca cgccggctga gcacgaattc cccagcgccc gcgccactga ctgcgacggc
     6241 acccgctgtt gccgagacgc cacgcgagag cgccgaggcg cgcaaggcgc gcaagcgggc
     6301 acaagccgag ttccgcagcg ctttggcacc cctgcgtgcg cggattgccg agctggagcg
     6361 agagctgaac cggttgctgg ccgagcgtgg tcggatcgag cagcaactgg cgaatccgcg
     6421 atccgatgcg gatcgcgccc gcctggtcca actggcgcag gcccgagcct caattgccaa
     6481 gcgcgtcagt gaggtggaaa gcacctggct gcaagcttcc gagcagctcg aagcacgtat
     6541 cgcagagcag caagacgttg actaaacacg tgttgctacc tagaatcgcc ggcctttgtc
     6601 ggtcggcccc tgaacacggc cagaccgcaa aaccgtgaac caagcgccgg gccgtccctt
     6661 cgatgggacg gcccggcgct tctcatacgc cgcattaccg tcgatctaga ctgttggagt
     6721 ttgaatggcc actaccggtc aattgattcg gcaaccccgc gcctcgcgtg ctgagaagac
     6781 caaggtcccg gcgctgggcg catcgcccca gaagcgtggc gtctgcaccc gcgtctacac
     6841 caccactccc aagaagccga actccgcgct tcgcaaggtg gcgcgtgtgc gactgacgaa
     6901 tggctacgag gtcagcagct atatcggcgg cgaagggcac aatctgcagg agcactcggt
     6961 ggtgctgatc cgcggtggtc gcgtcaagga tctgccaggt gtgcgctatc acaccgtgcg
     7021 cggcacgctc gactgcgccg gtgtcggtaa ccgcaagcag ggccgttcca agtacggcgc
     7081 caagcggccc aagaaataac gaggtatcaa gaatcatgtc ccgtcgtgcc gctgctccga
     7141 ctcgcaccat cctgccggac ccgaaattca acagcgatat gctggcgaag ttcatgaacg
     7201 tcgttatgca cagcggcaag aagtccgccg ccgagcacat tctctatggc gcgatcgagc
     7261 gcatcggtga gaagaccggt cggcaggggt ccgaagccat cgaactgctc tcgcaggcgc
     7321 tcgagaacgt gaagccggtg gtcgaggtca agtcgcgccg cgtgggtggt gcgacctacc
     7381 aggtaccggt cgaagttcgg gccagccgac gccagacact ggcgatgcgc tgggtgatcg
     7441 aggctgcacg tgcgcgcagc gagaagtcga tggcgtttcg cctggcgcac gagctgctgg
     7501 atgcagccga aaaccgtggt gcggctgtgc gcaagcgcga agacacccac cgtatggcgg
     7561 aggccaaccg agcgttcgcg cattaccgct ggtagcgcgg tagtggttct ttccacaagg
     7621 gagcaggagt aaatcg
//
LOCUS       DATMRM010000321         7336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1761428, whole genome shotgun sequence.
ACCESSION   DATMRM010000321 DATMRM010000000
VERSION     DATMRM010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761428"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /gene="gmd"
                     /locus_tag="VNZ06_09605"
     CDS             <1..886
                     /gene="gmd"
                     /locus_tag="VNZ06_09605"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWW21047.1"
                     /translation="TNLIRIVQHVQPDELYNLAAQSHVAVSFETPEYTANSDALGTLR
                     LLEAIRILGLTKKTRFYQASTSEMFGRVQEVPQKETTPFYPRSPYGAAKVYGYWITVN
                     YREAYGMYACNGILFNHESPIRGETFVTRKITRGLARIAAGLQQTLYLGNLDSLRDWG
                     HARDFIRAQWLILQQAEPDDFVIATGEQHSVREFVTRAGARLGMNIEWRGQGVDEQGI
                     DTKTSRAVVKVDPRYFRPTEVDTLLGDPTKAKRTLGWSPEITFEQLVDEMVDGDLGLA
                     KRDALIAREGFKTFHHHE"
     gene            879..1820
                     /locus_tag="VNZ06_09610"
     CDS             879..1820
                     /locus_tag="VNZ06_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HWW21048.1"
                     /translation="MNKSSKIFVAGHGGLVGAAVVRRLQSAGYSGILTRSRAELDLTN
                     QSAVQRFFADTRPDYVVLAAAKVGGIQANASHPAEFIQDNIVIQANVIDAAYRNGAHK
                     LLFLGSTCIYPKHAPQPMSEDCLLSGPLEPTNEWYAVAKIAGLKTCQAYRRQYGMDAI
                     SAMPTNLYGPEDNFSLENSHVLPALIRKFHDAKVRGDAKVTIWGTGTPRREFLYIDDC
                     ADALVFLLENYSDESIVNVGVGNDLSIHELAELVKSSVGFQGGLEFDTSKPDGTPRKL
                     VDVSRLTSLGWKAKTSLADGIRLTYEWFAAHHHRLRL"
     gene            complement(1843..3189)
                     /locus_tag="VNZ06_09615"
     CDS             complement(1843..3189)
                     /locus_tag="VNZ06_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013358747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWW21049.1"
                     /translation="MNQPAKRKARILVVDDDPGLLRLLTIRLRAEQYDVEAMENGVQA
                     LAAATRFRPDLVISDLRMDQLDGIGLLKELQNRWPGLKVILLTAHGTIPDAVQATQMG
                     AFGFLTKPVEKQELLDQVQKALKISGFVDNDQDWRSDFMTRSPVMEEKLAQAHMVAGT
                     DARVLITGESGTGKELLARAIHKASPRRNRPFVSINCSAMAENMLESELFGHAAGAFA
                     GATDDHRGLFQTAEGGTLLLDEIGDMPMRLQVKLLRALQENLIRPVGSTQAIPANVRV
                     ISSTHSDLQQQMAGGQFREDLYYRLNVVHIEMPPLNKRREDIPVLVAHFLATITQESG
                     TRKIYAPEAVELLATSDWPGNVRQLQNVVRQNVALSQTPIIPVELVQQSIGGAQGKLP
                     SFDEARDEFTRSYLSQILQITGGNVSQAARLAKRNRTDFYKLLGRHQLVPDDFKGR"
     gene            complement(3186..3815)
                     /locus_tag="VNZ06_09620"
     CDS             complement(3186..3815)
                     /locus_tag="VNZ06_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21050.1"
                     /translation="MRRKVLMLLALCLSACTIAPATMPVAPAPRSDNSVSEDRDAAAA
                     MLVGSIFQNMQHLAQAPPAEQAEILSAARASFERTPQGSVQLRYAMLLAAPGHAGRDL
                     NLSQTLLRQLTAQPETLVPIERAVAAIQLADIDRELGLKAENDRMQMDAQRGDRERNT
                     ASQRRLQAEMDENARLRKQLEDAQAKLDAIANIERNLTDRKTPGEGRPQ"
     gene            complement(3805..5277)
                     /locus_tag="VNZ06_09625"
     CDS             complement(3805..5277)
                     /locus_tag="VNZ06_09625"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWW21051.1"
                     /translation="MPWLLPKSLNGLLLLGLGILAGPLLLAIVHAALQMRHLTDASQR
                     LVADSVQTTRQSQDMFTHIGSVERSARLYQVLGESQLLSFREADDKLGATVALLRGRL
                     QDSNARSQLQLLLDEQARIRAIIDKGRAPANRAEVSAELDRSFGNLDAIAAGIVKLVN
                     QQIDADVGTLQIGTDRAQRELFLESTLLVPLVLIVVLIFALRLSRPLRQIDRAIGELG
                     RGSFSNVVTVDGPVDLERLGHQLEWLRNRLLELAHERNRFLRHMSHELKTPLANIREG
                     TELLMDGAVGELDSGQREVTEILRDNGIKLQRLIENLLSFSAWQSNNTGLEVSEFRLR
                     PVVKQVLENQQLTLVSQRVRLDVLIEDVTLVADRGKIRLILENLLSNAIKYSPRGGVI
                     VVRAGASGEDLQLEVADSGPGIAPEERARIFDAFYTGRAPSGHVKGTGIGLSVLNEFV
                     NVHQGTVEIVDGEFPGAHFRIRMPLRASIPDPNKKQADAA"
     gene            complement(5361..6350)
                     /gene="galE"
                     /locus_tag="VNZ06_09630"
     CDS             complement(5361..6350)
                     /gene="galE"
                     /locus_tag="VNZ06_09630"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HWW21052.1"
                     /translation="MSKAILVTGGAGYIGSHTLLQLQARRERVVVIDNLSTGFRQAVR
                     AAPLIVGDIGDRALVAQALREHDVDTVMHFAANTIVPESVRDPLKYYANNTCAARNLL
                     EACMQQGVRQFVFSSTAAVYGTPPSGVAREDMPLAPINPYGTSKLMTEWILRDLAAVS
                     DFRYVSLRYFNVAGCDPEGRIGQSTRKATLLIKVACEAIVGKRSHVSIFGTDYDTPDG
                     TGVRDYIHIADLARAHLDALDYLRRDGSSTILNAGYSHGYSVRQVLDGVQRIAGQKLT
                     IREEPRREGDPPSLVAEATRIRSQLGWSPQLDNLDAIIESALRWERQLQREPW"
     gene            6462..>7336
                     /locus_tag="VNZ06_09635"
     CDS             6462..>7336
                     /locus_tag="VNZ06_09635"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HWW21053.1"
                     /translation="MLDAAEALELAPDGRLFALNSYENRVYRVGRVDAEPVVLKFYRA
                     QRWSDQQILEEHAFGCELAAAEIPVVAPLQLQGLTLHRHKAFRLAAFPLCAGSAPELD
                     QPGARELLGRTLARIHALGALRRFQYRPSLERAMLGTRARASLLASPLLPAHMKGRYE
                     EVSSLLLRGIQRAFLRATPVPMIRLHGDCHLGNILWQARGPLFVDLDDCLNGPRIQDL
                     WMFLSGNADEQRGQWAEIVEGYRQFGTIDFRELQLVEPLRALRMLNHAAWVAERWQDP
                     AFPRAFPWAAEPRYW"
BASE COUNT         1355 a         2237 c         2284 g         1460 t
ORIGIN      
        1 gaccaacctg attcgcatcg tgcagcacgt gcagccggat gaactctaca atttggcggc
       61 gcaaagccac gttgcggtgt cattcgaaac gcccgaatac actgccaatt ccgatgcgct
      121 gggaacacta cggttgctgg aagcgatccg tattcttggt ctcacgaaga aaacccgttt
      181 ctatcaggcc tcaacctccg agatgttcgg cagggtgcag gaagtgccgc aaaaagaaac
      241 cacgcccttc tacccccgtt ctccgtatgg ggcggccaaa gtctacggtt actggattac
      301 cgtcaattat cgcgaggcct acggcatgta tgcctgcaac ggcatcctgt tcaaccacga
      361 gtcccccatc cgaggcgaga ccttcgttac tcggaaaatc acccgtggcc tggcgcgcat
      421 tgcagctggg ctgcagcaga ccctctacct ggggaacctc gactcgctgc gcgactgggg
      481 acatgcgcgc gacttcatcc gggcgcaatg gctgatcctg caacaggccg aaccggatga
      541 tttcgtgata gccacgggcg agcagcattc agtcagggag ttcgtcacgc gcgccggggc
      601 gcgtctggga atgaatattg agtggcgggg ccagggtgtc gatgagcaag ggatcgatac
      661 caagacgtcg cgcgccgtcg tcaaggtgga tccgcggtac tttcgtccta ccgaggtaga
      721 tactctgctg ggagatccca cgaaggcgaa gcggactctt ggctggtcac cggagatcac
      781 tttcgagcag ctcgtcgacg agatggtgga cggcgacctg ggacttgcga agcgtgatgc
      841 cctgatcgct cgcgagggtt ttaagacctt tcatcatcat gaataagagc tcaaagattt
      901 ttgtagcggg tcacgggggc ctggtcggtg ccgcagtagt acgacggctg cagtcggcgg
      961 ggtattccgg catcctgacg cgcagccgtg ccgagctcga tctcacgaat cagagtgcgg
     1021 tgcagcgttt ttttgccgat acccgaccgg actacgtcgt gctggccgcc gccaaggtgg
     1081 gcggaattca ggccaatgca agccaccccg ccgagttcat acaggacaac atcgtcatcc
     1141 aggccaacgt gatcgacgcg gcttaccgca atggcgcaca caagcttttg tttctcggct
     1201 ctacctgcat ttatccgaag catgcacctc agccaatgtc cgaggactgc ctgctgagcg
     1261 ggccgcttga gcccaccaac gaatggtatg cggtcgcaaa gatcgcgggg ctcaagacct
     1321 gccaggcgta tcggcggcag tacggcatgg acgcgatttc agccatgccc accaatctgt
     1381 acggtcccga ggacaacttc agtctggaga actctcacgt actcccggcg ctgattcgca
     1441 agtttcacga cgccaaggtg cgcggcgatg ccaaggtgac gatctgggga accggaacac
     1501 cgagacgcga gtttctctac attgacgact gcgccgacgc tctggtgttc ttgctcgaga
     1561 actacagcga tgagagcatt gtcaacgtcg gcgtgggcaa cgacctgagc attcacgagc
     1621 tcgccgagct cgtgaagtca agcgtcggtt ttcaaggagg gctggagttc gataccagca
     1681 aacccgacgg cactccgcgc aagctggtgg acgtctcgag gctcacgagc ctgggctgga
     1741 aagcaaaaac ctcgctggcg gatggcatcc ggctcaccta cgaatggttt gccgctcatc
     1801 accaccggct gcggctctag agctctaggc gccagagtct ctctatctgc ctttgaagtc
     1861 gtccggcacc agctggtgtc ggcccagcag tttgtagaaa tcggtgcggt tgcgtttcgc
     1921 cagccgtgcg gcctggctca cgttgccgcc ggtgatctgc aggatctgcg atagatagct
     1981 gcgggtgaat tcgtcgcgcg cctcatcgaa ggacggcagc ttgccttgtg cgcccccgat
     2041 cgactgctgc accagttcca ccggaatgat cggcgtctgc gacaaggcga cattctggcg
     2101 cacgacgttc tgcagctgtc tgacattgcc tggccagtcg ctcgtggcga gcaactcgac
     2161 cgcctcgggc gcgtaaatct tgcgggtgcc gctctcctgt gtgatcgtcg ccagaaagtg
     2221 cgcgaccagc accggaatgt cctcgcgtcg cttgttgagc ggcggcatct cgatatgtac
     2281 cacgttcagg cgatagtaaa gatcctcgcg aaactgtcca cccgccatct gctgctgcag
     2341 atcgctgtgg gtcgacgaaa tgacacgcac gttggcggga atggcctggg tacttcccac
     2401 tggccgaatg aggttttcct gcaacgcccg taacagcttg acctgcaggc gcatcggcat
     2461 gtcgccgatc tcatccagca gcagtgtgcc gccttcggca gtctggaaca gaccgcgatg
     2521 atcgtcggtc gccccggcga aggcgccggc ggcatgtcca aacaactcgc tctcgagcat
     2581 gttctcggcc atggcgctgc aattgatcga cacgaacggg cgattgcgcc gggggctggc
     2641 cttgtggatc gcccgcgcca gcagctcttt acccgtgccg ctttcgccgg tgatcaacac
     2701 gcgcgcgtcg gtgccggcca ccatgtgtgc ctgcgccagc ttttcttcca tgacgggact
     2761 gcgggtcatg aagtccgaac gccagtcctg gtcattgtcc acgaacccgg agatcttcag
     2821 cgccttctgc acctgatcga gcagctcctg tttctctacc ggcttggtga ggaatccgaa
     2881 cgctcccatc tgggtcgcct gcacggcgtc gggaatggtg ccgtgcgcgg tcaggagaat
     2941 cactttcagg ccgggccatc gattctgcag ttccttgagc agaccgatgc cgtcgagctg
     3001 atccatgcgc aggtccgaaa tcaccagatc agggcggaag cgcgtggcag cggccagggc
     3061 ctgtacaccg ttttccatgg cttcgacgtc atattgttcc gcgcgcaggc ggatcgttag
     3121 caggcgcaac agacctggat cgtcatcgac gaccagaatg cgtgccttgc gcttggcggg
     3181 ctgattcatt gtggccgtcc ttcgccgggt gttttgcggt cagtgagatt gcgctcgata
     3241 ttcgcaatgg cgtcgagttt tgcctgcgcg tcctcgagct gcttgcgcaa tcgcgcgttc
     3301 tcgtccatct cggcctgcag tcggcgttga gaggctgtat tgcgctcacg atccccgcgt
     3361 tgagcgtcca tctgcattcg atcattctct gctttgaggc cgagctcccg atcgatatca
     3421 gccagttgaa tcgcggccac cgcgcgctcg atcggcacca gggtctccgg ttgcgccgtg
     3481 agctgccgca gcagcgtctg tgacaggttc agatcgcgtc ccgcatgccc cggcgcggcc
     3541 aatagcatcg catagcgaag ctgcacgctg ccctgagggg tgcgctcaaa ggaggctcgc
     3601 gccgctgaga ggatttccgc ctgctccgcc ggcggggcct gggccaggtg ctgcatattc
     3661 tgaaagatgc tgccaacgag catcgcagcg gccgcgtcgc ggtcctcgct gacactgttg
     3721 tcgctgcgtg gcgccggcgc cacgggcatg gtcgcaggag cgatcgtgca ggcactcagg
     3781 cacagcgcaa gcagcatcag gaccttacgc cgcatcggcc tgcttcttgt tggggtccgg
     3841 gatcgaggcg cgcagtggca tgcgaatgcg gaagtgcgcc cccgggaact cgccgtccac
     3901 aatttcgacg gtgccctgat ggacgttgac gaactcgttc agcaccgaga gcccgatgcc
     3961 ggtccccttg acgtggccgc tgggcgcacg cccggtgtag aaggcatcga atatacgggc
     4021 gcgctcctcc ggagcgatcc cgggaccgct gtccgcgact tccagctgca gatcttcgcc
     4081 gctggcaccg gcccgtacga cgatgacgcc gccgcgcggt gagtacttga tggcattgga
     4141 caatagattt tccagtatca atcgaatctt gccccgatcc gcgaccaggg tgacgtcttc
     4201 aatcagcacg tccagacgca ctcgctgaga caccaaggtg agctgttggt tctcaagaac
     4261 ttgcttcacc accggccgca accgaaactc cgagacctcc agtccggtgt tgttgctctg
     4321 ccacgcactg aagctgagca ggttctcaat cagacgctgc aatttaatgc cgttgtcgcg
     4381 caggatctcc gtgacctccc gctgtccgga atccagctcg ccaactgcac cgtccatcag
     4441 caattctgtg ccctcgcgga tgttagcaag aggggtcttc aattcgtgcg acatatgccg
     4501 cagaaatcgg ttgcgctcgt gcgccagttc caacaatcga ttgcgcagcc attcgagctg
     4561 atggcccagt cgttccagat ccaccggccc gtctaccgtg acgacgttgg agaagctgcc
     4621 ccggcccaac tcgccgatcg cacggtcgat ctgacgcaga ggtcggctca ggcgcagcgc
     4681 gaaaatcagc accacgatca ataccagcgg caccagcagt gtggactcga ggaacagttc
     4741 acgctgcgct cggtcggtcc cgatttgcag cgtaccgaca tccgcatcga tctgctgatt
     4801 gacgagtttg acgatgccgg cggcgatcgc gtcgaggttg ccgaatgagc ggtccagttc
     4861 ggcgctcacc tcggcgcggt tggccggcgc gcgccctttg tcgatgatcg cgcgaatgcg
     4921 cgcttgctca tccagcagca gctgcaactg gctgcgggca ttggaatcct gcaatcggcc
     4981 gcgcaacagc gcgacggttg cgccgagttt gtcgtcagct tcgcgaaagc ttagcagctg
     5041 cgactcgccc agtacctgat acagccgagc ggagcgctcg accgaaccaa tgtgcgtgaa
     5101 catgtcttgc gactgccgcg tagtctgcac gctgtctgcg accaagcgct ggctggcatc
     5161 ggtcaggtgt cgcatttgca gtgcggcgtg cacgattgcg agcagcagcg ggcccgccag
     5221 gatacccaga cccagcagca gcagcccatt gagagatttc ggaagtagcc agggcatcga
     5281 aatattctaa gccactaatg cgcgagatct agcgtgacct tcggtgaccc aggtcgcacg
     5341 gacgctaaat ttgagggcaa ttaccacggt tcgcgttgca attggcgctc ccagcgcagc
     5401 gcactttcga tgatcgcgtc gagattgtcg agctggggac tccagccgag ctgcgagcga
     5461 atgcgcgtgg cttcggccac cagactgggc ggatcgcctt cgcgccgcgg ctcttccctg
     5521 atcgtcagct tctgccccgc gattcgttgg accccatcca gtacctgccg cacgctatag
     5581 ccgtggctgt aaccggcatt gagaatcgtc gagctgccgt cgcgacgcag gtagtccagc
     5641 gcgtccaggt gtgcccgcgc caggtcggcg atgtgaatgt agtcgcgtac tccggtgcca
     5701 tcgggcgtgt catagtcggt gccaaagatt gaaacatgtg aacgcttacc tacgatcgcc
     5761 tcacaggcaa ctttgatcaa cagggtcgcc ttgcgtgtgg attgcccgat gcgtccctcg
     5821 ggatcgcagc cggcgacatt gaaatagcgc aggctgacgt aacgaaaatc gctaacggcg
     5881 gccagatcgc gcaggatcca ctcggtcatg agcttcgagg taccgtaggg attgatcggc
     5941 gcgagcggca tatcttcgcg cgcgacaccg gacggcggcg tgccgtagac cgccgccgtg
     6001 gacgaaaaca cgaactggcg cacgccctgt tgcatgcacg cctcaagcag gttgcgagcg
     6061 gcacaggtgt tgttggcgta gtacttgagc ggatcgcgca ccgattccgg tacgatggtg
     6121 ttggcagcaa agtgcatgac ggtgtccaca tcgtgctcgc gcagcgcctg ggccacgagc
     6181 gcgcggtcgc cgatgtcgcc gacgatcagc ggtgccgcgc gcacggcctg gcgaaagccg
     6241 gtagagagat tatcgatcac gacgacgcgt tcgcggcgcg cttgcagctg caggagcgtg
     6301 tgactgccga tgtagccggc gccgcccgtg accaggatcg cctttgacat agcgccgtga
     6361 cctcaccatg tatagattgc aaggtcgcga ataataacgg catttggttg ccatcgtacg
     6421 tggatatacg cacacccttt gcagaattga cgccggacgc ggtgctggac gccgcggaag
     6481 cgctggagct ggcgcctgat gggcggctgt tcgcgctgaa tagctatgaa aacagggttt
     6541 atcgcgtggg acgcgtggac gctgagccgg tcgtgttgaa gttctatcgc gcccagcgct
     6601 ggtcggatca gcagattctc gaagaacacg catttggctg cgagctggcg gcggcggaaa
     6661 ttccggtcgt ggcgccgctg caactgcagg gtctgacatt gcatcgtcac aaggcatttc
     6721 ggctggccgc ttttccgctg tgcgcgggct cggcaccaga actcgatcag cccggagcgc
     6781 gcgagctgct gggccgcacg ctggcgcgca tccacgcgct gggagcgctg cggcgatttc
     6841 agtatcgtcc gagtctggag cgcgcgatgc tcggaacgcg cgcgcgcgcc agcctgttgg
     6901 cttccccgtt gctgccggcg cacatgaaag ggcgttatga agaagtgagc tcgctgctgc
     6961 tgcgtggaat ccagcgcgcg ttccttcgcg ccacgccggt gccgatgatc cgcctccacg
     7021 gagactgcca cctcggcaat atcctgtggc aggcgcgtgg cccgctgttc gtcgatctgg
     7081 atgattgttt gaatggtcca cgcatccagg atctgtggat gtttctgtcc ggcaatgcgg
     7141 atgagcagcg ggggcaatgg gccgaaatcg tcgaaggata tcgtcagttt ggaacgatcg
     7201 acttccggga gttgcagctg gtggagccgc tgcgagcgct gcgcatgctc aatcatgccg
     7261 cgtgggtggc cgaacgctgg caggatccgg cctttccgcg cgcgtttccg tgggctgccg
     7321 aaccgcggta ctggga
//
LOCUS       DATMRM010000322         2639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3900286, whole genome shotgun sequence.
ACCESSION   DATMRM010000322 DATMRM010000000
VERSION     DATMRM010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3900286"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VNZ06_09640"
     CDS             complement(<1..394)
                     /locus_tag="VNZ06_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWW21054.1"
                     /translation="MNTLQGKTALVTGAASGIGRASAQLFAAEGAAVVALDRAPEVEA
                     TVASIKSAGGRAIALVKDSSSEADIAAAVATALSQFGALDVCYANAGVSGGMVPMPEQ
                     TADHWTEILKINLIGAFLAVKHASAEMVK"
     gene            449..1882
                     /locus_tag="VNZ06_09645"
     CDS             449..1882
                     /locus_tag="VNZ06_09645"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWW21055.1"
                     /translation="MPKSTGLRPRAVYRHAELDRILNPRSIAIVGASPKPGSFGDRVL
                     NHISTFSGDIYLVNPKYDRIGERDCYASVKDLPGSPDCVAVAVPRESVEPIVCDAASV
                     SAGGVLLYASGYAEMQRSEHIEQQQRLSAIARESGLKIIGPNCLGIVNYLKRFRLSFA
                     EYPKPRDQLGALIGIASQSGAMSQAMSQALERGIGISHALSAGNQSDVDVADYVSYLA
                     DEPGCRAIACAFEGMSSPERMLEAALIAWRAGKPLLINKLATGRAGAEAALRHTGSDA
                     GVDQAWRDVFERGGAIVLDEFEGLMEAAAFFAKAPPPAARGVAVVATSGGAAILCADK
                     ADEHGVPLPQPSERVHRILASLIPEFGCSLNPCDVTGQVVNNPQSLWECADALLSDPA
                     FGALIAPQTVVSEMNWQPRIDALQTVSAQRGKITCNVLLSNWLQGPGLYESERHTHIA
                     VFHSMDRCFRTLAAWHRRADRMARGEP"
     gene            complement(2002..>2639)
                     /locus_tag="VNZ06_09650"
     CDS             complement(2002..>2639)
                     /locus_tag="VNZ06_09650"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HWW21056.1"
                     /translation="TLFWTAIPYALQPEGAAHMFSGQPAEALKAFREDRAAFRSNIPP
                     MRPAESIQGMKIYLGQLEAMLHDGRAWLLGAAASIADFSVYHCLWFIHRGGPVAAIFD
                     NYPQVQGWYARMKAIGHGVSEPMDSAAAINIAASSKHAPVSAEEFLDTHRLPFGSRVS
                     VAATDYGTDPMVGELVISRPEEIGVRRTDPRAGTVVVHFPRLGFEVRKAEQ"
BASE COUNT          470 a          882 c          820 g          467 t
ORIGIN      
        1 gcttgaccat ttccgcgctc gcgtgtttga cagcgaggaa cgcgccgatc aggttgatct
       61 tcaggatttc cgtccagtga tccgcggtct gctcaggcat gggcaccata ccgccactga
      121 cccccgcatt ggcgtaacac acatcgagcg caccgaattg gctcagcgcc gtggcgacgg
      181 ctgcggcaat gtccgcttcc gaggacgaat ccttcaccag cgcgatcgcg cgcccacccg
      241 ccgacttgat cgacgcgacg gtcgcttcca cttcaggggc cctgtcgagt gcgacgacgg
      301 ccgcgccctc ggcggcaaac agctgcgcac tggcgcgtcc gataccgctg gccgcacccg
      361 tcacgagcgc agtcttgcct tgcagggtgt tcatgagatc ctcgttttct tatgctgaca
      421 tgatgaggtt atcgtaaatt gacggcacat gcctaaatcg accggattgc gacccagagc
      481 cgtctatcgg cacgctgaac tcgaccgcat cctgaacccc cgctcaatcg ccatcgtcgg
      541 ggcctcaccg aagcccggct cctttggcga tcgggtgctc aatcatatct cgaccttctc
      601 cggcgatatc tacctggtga atccgaaata tgaccgcatc ggcgagcgcg actgctatgc
      661 ctcggtgaag gacctgccgg gctcccctga ctgcgttgcc gtggcggtgc cgcgcgagtc
      721 ggtcgagccg atcgtgtgtg atgcagcctc ggtcagcgcc ggcggcgtgc ttctttacgc
      781 ctccggctat gctgagatgc agcgcagcga gcacatcgag caacagcaac gcctcagcgc
      841 gatcgcgcgt gagagcggac tgaagatcat cggtcccaac tgcttgggga tcgtcaacta
      901 cctgaagcgc ttccgcctgt cattcgccga atatcccaaa ccgcgcgatc agctcggcgc
      961 gctgatcggc atcgccagtc agtccggcgc gatgtcgcag gcgatgtcgc aggcgctcga
     1021 acgcggcatc ggtatcagcc atgcgctgtc ggcgggcaac cagtcggacg tcgatgtggc
     1081 cgactacgtg tcctatctgg ccgacgaacc aggctgtcgg gccatcgcct gcgcgttcga
     1141 gggcatgtcc agtcccgagc gcatgctcga ggccgccctg atcgcatggc gcgctggcaa
     1201 accgctgctg atcaacaaac tcgcgaccgg ccgggcggga gccgaggccg ccctgcggca
     1261 caccggcagc gacgccggcg tcgatcaggc gtggcgtgac gtgttcgagc gcggcggcgc
     1321 cattgtgctg gatgagttcg aaggcctgat ggaggctgcc gcattcttcg ccaaagcacc
     1381 gccaccggcc gcgcgcggcg tcgcggtcgt cgccacctcc ggcggggccg cgatcctgtg
     1441 cgccgacaag gccgatgagc acggtgtgcc gctgccacag ccgagtgaac gcgtgcaccg
     1501 gatactggcc tcgctgatcc ctgaatttgg ttgttcgctg aacccatgcg acgtcaccgg
     1561 ccaggtggtc aacaatccgc aatcactctg ggagtgcgcg gacgccttgc tctcggatcc
     1621 tgcctttggt gcgctgatcg cgccgcagac ggtggtctcg gaaatgaact ggcaaccacg
     1681 catcgacgcg ctgcagacgg tgtcggcgca gcgcggcaaa atcacctgca acgtgctgtt
     1741 gtccaactgg ctgcagggcc cgggactcta tgagtccgag cgccacacgc acatcgcggt
     1801 gttccactcg atggatcgct gctttcgcac cctcgccgcc tggcaccgtc gtgccgatcg
     1861 gatggcacgc ggcgagccat aaaataaggc gccctgatca catcgggcgc cgtgagccaa
     1921 gcaaagactg ccgagtgccg ctatttcgtg atgccgttcc acgtcagcga ccggttgcgc
     1981 gggcgcgcaa cctgattggg tctactgctc ggccttgcga acctcgaatc cgagccgcgg
     2041 gaagtgcacc accacggtac cggcacgcgg gtcagtgcgg cgcacgccga tctcctcagg
     2101 ccgggaaatc accagctcgc caaccatcgg atcggtgccg taatcggtcg ccgccacgct
     2161 gacgcgagag ccgaacggca gacgatgggt gtccaggaat tcttccgcac tgaccggcgc
     2221 gtgcttcgag gacgctgcaa tgttgatcgc ggccgcactg tccatcggct cagacacgcc
     2281 gtggccgatg gctttcatgc gcgcatacca gccttgcacc tgaggatagt tgtcgaagat
     2341 cgcggccacc ggaccaccgc ggtggatgaa ccacaggcag tgatagaccg agaagtctgc
     2401 aatcgatgcc gcagctccga gcagccaggc gcgtccatcg tgcagcatcg cctcgagctg
     2461 ccccagatag atcttcatgc cctggatcga ctcggccggc cgcatcggag gaatgttgct
     2521 gcgaaacgcg gcgcgatcct cacgaaaggc cttcagcgct tccgcaggct gaccactgaa
     2581 catgtgggcc gcgccctccg gctgcagcgc atagggaatc gcggtccaga acagcgtga
//
LOCUS       DATMRM010000323         5933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4545174, whole genome shotgun sequence.
ACCESSION   DATMRM010000323 DATMRM010000000
VERSION     DATMRM010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4545174"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VNZ06_09655"
     CDS             <1..492
                     /locus_tag="VNZ06_09655"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HWW21057.1"
                     /translation="EQGLELGRAQGIREVAERAQSGLGALAKVATAAEEAFALESAQL
                     ADACTEIVAEAFVKLAGRRLGTRKAAVSVVLQVLKRIKDGRELTIKVSAADLPMLQSQ
                     ESDIARVLGTRRWTLMADARVSLGGCLVESVLGTLDGRLEVQLRELFETLRAARVSRL
                     EVE"
     gene            489..1838
                     /locus_tag="VNZ06_09660"
     CDS             489..1838
                     /locus_tag="VNZ06_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HWW21058.1"
                     /translation="MNRPAHLLSSLRSADWIRRTGQVVRFVGLSVESRGPDAHLGEIC
                     EIQAHTSGARIPAEVIGLAEGRVMLMPDQDLYGIELGDEVTATGQRAHVLAGRHLLGR
                     VIDGNGAPLDGKPLSPGGLRYALYPNPINPLGRGLVCEVLETGVRAIDTLFTLARGQR
                     VGIFAGSGVGKSTLLGMLARQVQADVSVVALVGERGREVKAFVESGLSAAARARTVVV
                     AATSDQPPLIRRRAAFLATAVAEYFRDQGLHVCLTMDSLTRVAMAQREIGLASGELPT
                     ARGYTPSVFSLLPRLLERGGVRTEGGSLTALYTVLVEGDDIEDPIADNVRAILDGHIV
                     LSRQVAQRGRFPAIDVSHSISRLAATAAAAHEHQCANEAMELLALYESARDLIEVGAY
                     RAGANPALDRAVQRVPVLEKFLAQRPDEIEPRGAALAKLRMILEPQGGDGAAIRKTR"
     gene            1813..2295
                     /locus_tag="VNZ06_09665"
     CDS             1813..2295
                     /locus_tag="VNZ06_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21059.1"
                     /translation="MPRSARHDSATLRTLERHRGVAVEQAQAQHQQRATEQTRDEAAH
                     TQLQRSLESTYSSLRALVQPGEILAGPSLSLSYEYARSQVAQLQQAALIRERSRALVD
                     EAQAQLAARLEELKVIERLRERRRRGVARWVSRRNQWRIDELGTIKAWYREGLWRSVE
                     "
     gene            2301..2705
                     /locus_tag="VNZ06_09670"
     CDS             2301..2705
                     /locus_tag="VNZ06_09670"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HWW21060.1"
                     /translation="MNSNTATQSVTSTLTQQDFLSILLTQLQFQDPLKPMDDEQFVAQ
                     LAQFSALEVNTEESAKLDALISFASINQGVGLVGKSVVISANQGGINGAASATGTVTA
                     VSFDSGEPLLTVTTSSNTITNVPLTNVTLVHQ"
     gene            2762..3979
                     /gene="flgF"
                     /locus_tag="VNZ06_09675"
     CDS             2762..3979
                     /gene="flgF"
                     /locus_tag="VNZ06_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HWW21061.1"
                     /translation="MTILNAVYTGMSGMLAYQQGLTIIGNNVANLDTSGFKSTEPLFE
                     DLEFQTGTGGFAGSPGTPTIGGGVSVDDTHVSFAQGNEQATGDPLDAAVNGLGFFVVQ
                     RNGSDYYTRAGQFQFDQDGDLVQTTTGAKVMVSTSTSAIGSFNIKAYNSYAPVETTTV
                     ALSGNLASSGQTAPYVLPGITVVDGGGGTETLQATFTQDSANPLHWTVQVQDASNKII
                     GTGDLFFNADGSLATTTTPIVVTVTPSKVPAFKINLTFGTAGGFTGVTSLASNSSSQL
                     QVASQDGVQLGTLTGTSFGSDGSLTLTYSNGKTLNPAKLLLAQFEAPQQLKAVDNGLF
                     SAVGNALPVLGTALSTNFGSIVGGEVEMSNVDLSQEFTELIIIQRGYQASSQIASMAN
                     DMMQQLLTMGNQR"
     gene            3976..4821
                     /locus_tag="VNZ06_09680"
     CDS             3976..4821
                     /locus_tag="VNZ06_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor C-terminal
                     domain-containing protein"
                     /protein_id="HWW21062.1"
                     /translation="MSAAPAAIHAVPYLLLGESRQRQLQERLSELVMQWHRTWAPERS
                     TQPSVQLGPEGEAQTVRGEAWTYRVLSGADTLMQICVRADLLRILCGVGAGEGVFAAG
                     FAPAPASLAAELTQQILLALGQQVVRAALPAAECTVQRLVGTNCPDIVSGVRILSVFM
                     SADRSRPTALLTLSPTLTDALLGARPGPALREPMVGRRQASSEQLIRLRAVLGVATVS
                     WQELNALSVGDVIVLDQALSAPCSLMAGDEREIADVALGQMDNVMAAQVTRLRGAPLA
                     REMPK"
     gene            4847..5161
                     /locus_tag="VNZ06_09685"
     CDS             4847..5161
                     /locus_tag="VNZ06_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HWW21063.1"
                     /translation="MTQTVVNRLDMPALAAATGPKGQSLGQRLDLVEQVKVQLSVTLG
                     DAEVTMARLFALAAGDVVALDRQVDAPVDVRLHGKLIARGTLVAVGDKFGIRVTEIAT
                     PG"
     gene            5185..5592
                     /locus_tag="VNZ06_09690"
     CDS             5185..5592
                     /locus_tag="VNZ06_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21064.1"
                     /translation="MIVRALCIVALPLWAPAMSGASVDSPLPPPIAGMRAQPDVGLPS
                     ASRVIIGVAITFALAVGAIVLIRRFAPNAATRWGGGSTSSIKLLARTQLSRTLCAQVV
                     EFESTRILIVEAREGVGLTLLPKSETPGRPPSP"
     gene            5589..>5933
                     /locus_tag="VNZ06_09695"
     CDS             5589..>5933
                     /locus_tag="VNZ06_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21065.1"
                     /translation="MTGAAQRAAGFQRAAARRRYGVLLASVIGMLMASALMSTALAAA
                     PNSLGLSIEVGKPGAVPVADALKIILMLTALALAPGILIAMTSFTRTIIVLSMIRHGF
                     GMQDTPPNLVLLS"
BASE COUNT         1062 a         1806 c         2010 g         1055 t
ORIGIN      
        1 gaacagggac ttgaactcgg acgggcccaa gggatacgcg aggtcgcgga gcgcgcgcag
       61 tcgggccttg gcgcgctggc gaaagtggcc acggcggcgg aggaggcgtt tgcgcttgaa
      121 agcgcgcagc tcgcggatgc ctgcaccgag attgtggccg aggcgtttgt caaactcgcc
      181 ggccgacgtc tcggcacccg aaaggccgcc gtcagcgtgg tgctgcaggt gctcaagcgc
      241 atcaaggatg gccgggagct gacgatcaag gtcagcgcgg cggatctgcc gatgctgcaa
      301 tcgcaggaat ccgacatcgc acgggtgctc ggaacgcgtc gatggaccct catggctgat
      361 gcgcgcgtga gccttggcgg ttgcctggtg gaatcggtgc tcggcacgct cgacgggcgg
      421 ctcgaagtac agttgcgcga gttgttcgag acgctgcgag cggccagggt ttcacggctg
      481 gaggttgagt gaaccgtcca gcgcatctgc tgagcagcct gcgatcggcc gattggatca
      541 gacgcaccgg ccaggtggtg cgcttcgtgg gcctgagtgt ggagtctcgc ggtccggacg
      601 cgcacctggg cgagatctgc gagattcagg ctcatacttc gggcgcgagg attcccgcgg
      661 aagtcatagg acttgccgag gggcgtgtga tgttgatgcc ggaccaggat ctctacggca
      721 tcgagctggg cgacgaggtg actgccaccg gccagcgcgc gcatgtcctg gcaggcaggc
      781 atctgctcgg ccgtgtcatc gacggcaacg gcgcgccgct ggatggcaaa ccgttgtcgc
      841 cgggcgggct gcgctatgcg ctgtatccca accccatcaa ccctctgggc cgcggcctgg
      901 tgtgcgaggt gctcgagacc ggtgttcgcg ccatcgacac gctgttcact ttggcgcgcg
      961 gtcagcgcgt cggcatcttc gcgggtagcg gagtgggcaa gtccacgctc ctcggtatgc
     1021 tcgcgcgcca ggtgcaagcc gacgtatccg tggtcgcatt ggttggggag cgtggacgcg
     1081 aagtcaaggc cttcgttgaa agcggcctgt cggcagcggc gcgcgctcgc accgtcgtgg
     1141 tggcagccac ttccgatcag ccacccttga ttcggcggcg cgcggccttt ctggctaccg
     1201 ccgtggctga gtacttccgc gaccagggcc tgcatgtctg tctgacgatg gattcactga
     1261 cgcgcgtcgc gatggcgcaa cgtgaaatcg gactggccag cggggagttg cctacggcgc
     1321 gcggctacac gccctcggtg ttctcgttgc tgccgcgcct gctggagcgc ggaggcgtac
     1381 gaaccgaggg cggttcactg accgcgctgt acacggtgct ggtggaaggt gacgacatcg
     1441 aggatccaat cgcggacaac gtgcgcgcca ttctcgacgg acatatcgtg ttgtcgcgcc
     1501 aggtggctca acgcgggcgt tttccggcca tcgatgtttc ccacagcatc agccgcctcg
     1561 ccgcgaccgc ggcagcagct cacgagcatc aatgcgcgaa cgaggcgatg gagttgctgg
     1621 cgctgtacga gtccgcgcgc gatctgatcg aagtcggtgc gtatcgcgca ggggccaatc
     1681 cggcgttgga tcgcgccgtg cagcgggtgc cggtgctgga aaaattcctg gcacaacgcc
     1741 ccgatgagat tgagccccgt ggcgcggcgc tggccaaact gcggatgatt ctggagccgc
     1801 aaggaggcga cggtgccgcg atccgcaaga cacgatagtg cgacgctgcg caccctcgag
     1861 cgccaccggg gcgtcgcagt cgagcaggcg caggcacagc accagcagcg cgcaaccgag
     1921 cagacgcgcg acgaggccgc gcacacgcag ctgcagcgct cgctcgagtc cacttattca
     1981 tcgctgcgtg cgctggtgca accgggggag atactggcgg gaccgagcct gtctctgtcc
     2041 tatgagtatg cgcgatcgca ggtcgcgcag ttgcagcagg ccgcgctcat ccgggaacgc
     2101 agtcgcgctc tggtggacga agcgcaggca cagttggcgg cgcggctcga ggaattgaaa
     2161 gtgattgagc gtctgcggga gcgcagacgc cgcggggttg cacgctgggt ctcgcgccgc
     2221 aatcaatggc ggatcgatga gctgggtacg atcaaagctt ggtataggga gggactatgg
     2281 cggtcagtgg aataggcatc gtgaactcga acaccgcgac gcagtcggtg acatccacgc
     2341 ttacgcagca ggattttctg agcattctgc tcacccagtt gcagttccag gaccccctga
     2401 aaccgatgga tgacgagcag ttcgtcgcgc agctcgcgca gttcagcgcg ctggaggtca
     2461 ataccgagga gagcgcaaaa ctcgacgcgt tgatctcgtt tgccagcatc aatcaaggcg
     2521 taggcctggt tggcaagagt gtggtgatca gcgccaatca gggcgggatc aatggtgccg
     2581 ccagcgccac gggcacggtg acggccgttt ccttcgacag cggcgagccg ttgctcaccg
     2641 tgacgaccag cagcaacacc atcaccaacg taccactcac caacgtgaca ctggtccatc
     2701 agtagcaacc gaaccaccgt gtcgcgcggc acacggccat gactgagcga ggagagagca
     2761 catgacgatt ctgaacgctg tctacaccgg tatgtccggc atgctggcct accagcaggg
     2821 cctcacgatc attggcaaca acgtcgccaa tcttgacacc tcgggattca agtccaccga
     2881 accgttgttt gaggatctgg agttccagac cggaaccggc gggtttgccg gcagtccggg
     2941 taccccgacg atcggcgggg gtgtgagcgt ggacgacact cacgtgtcgt ttgcgcaggg
     3001 gaacgagcag gcgaccggcg atccgctgga cgcagccgtc aacggcctcg ggttcttcgt
     3061 cgtgcagcgt aacggcagcg actactacac ccgcgccggc cagtttcaat ttgatcagga
     3121 tggtgatctg gtgcaaacca ccaccggcgc gaaggtcatg gtctcgacct cgacctcggc
     3181 catcggaagt ttcaacatca aggcctataa ctcgtacgca cccgtggaga cgaccaccgt
     3241 cgcgttgagt ggcaatctgg cgagcagtgg acagacagca ccctatgtgc tgcccggaat
     3301 caccgtggtg gatggtggcg gtggcaccga aaccctgcag gcaaccttca cgcaggacag
     3361 cgccaatccg ctgcattgga ccgtacaggt acaggatgcc agcaacaaga tcattggcac
     3421 gggcgatctg ttcttcaatg ccgacggctc gctggcaacc acgacgaccc cgatcgtcgt
     3481 taccgtgacg ccgagcaagg tgccggcgtt caagatcaat ctgaccttcg gtaccgccgg
     3541 cggcttcacc ggcgtgacca gcctcgcgtc caattcgagc tcacagctgc aagtggcctc
     3601 gcaggatgga gtgcagctcg gcacgctcac cgggacgtcg ttcggctccg acggttctct
     3661 gacgctcacc tattccaatg gcaagactct caaccccgcc aaactgctgc tcgcgcagtt
     3721 cgaggcgccg cagcagctca aggccgtgga caacggtctg ttctccgcgg taggcaacgc
     3781 gctgccggtc ctggggacgg cactcagcac caactttggc agcatcgtgg gcggtgaggt
     3841 agagatgtcc aatgtggatc tgtcgcagga gttcacggag ctgatcatca ttcagcgcgg
     3901 ctatcaggca agctctcaga tcgcctccat ggccaatgac atgatgcagc agcttctgac
     3961 gatggggaat caacgatgag tgcagcgccc gctgcgatac atgccgtgcc ttatctgctg
     4021 ctgggggaga gtcgccagcg acagctgcaa gagcggctga gcgaactcgt gatgcagtgg
     4081 catcgcacct gggcgccaga gcgttcgacg caaccttcgg tgcagcttgg gcccgaaggc
     4141 gaagcgcaaa cggtgcgagg ggaggcgtgg acgtaccggg tgctcagcgg cgccgacacg
     4201 ctcatgcaga tctgcgtgcg cgcggatctc ttgcggatcc tgtgcggtgt gggtgcgggc
     4261 gagggcgtgt ttgcggcggg tttcgcaccc gcaccggcga gtcttgcagc ggagctgacg
     4321 caacaaatcc tgctggcgct gggccagcag gtcgtgcgcg cggcactacc cgcagccgag
     4381 tgcacggtgc agcgattggt cggcacgaac tgtccggata tcgtcagcgg cgtgcgcatc
     4441 ctgagcgtgt tcatgagcgc tgaccggtcg cgaccgacgg cgttgttaac gctctcaccg
     4501 acgctgaccg acgcgctgct gggtgcacgg ccggggccgg cgctgcgcga gccgatggtg
     4561 ggacgtcgcc aagccagcag cgagcagttg atccggctgc gcgcggtgct cggcgtcgcc
     4621 acggtgtcgt ggcaggagct caatgcgctg tccgttggag acgtcatcgt actggaccag
     4681 gcgctgtcag cgccgtgttc gctcatggcg ggtgatgagc gcgagatcgc cgacgttgcg
     4741 ctggggcaga tggacaacgt catggccgcg caggtaacgc gccttcgagg tgcgccactc
     4801 gcccgtgaga tgccgaaatg aataccttta gatgaaggac ggtgaaatga ctcaaacagt
     4861 ggtgaatcga ctcgacatgc cggctctcgc tgctgcgacg ggacccaagg gacaatccct
     4921 ggggcagcgc ctggatctgg tcgagcaggt caaagtgcaa ctatcggtca ccctcggaga
     4981 tgcggaggtc acgatggctc gcctgtttgc actggcagcg ggtgatgtcg tggcgctcga
     5041 tcggcaggta gacgccccag tggatgtgcg cctgcacggc aagctcattg ctcgaggcac
     5101 actggttgcc gtcggtgaca agttcggaat ccgcgtaacg gaaattgcca ctcccggctg
     5161 agtcgcgccg tgcccctgag actcatgatt gtgcgcgcgc tgtgcatcgt ggcgttgccg
     5221 ctgtgggcgc cggcgatgtc cggcgccagt gtcgattcgc cactgccgcc tcccattgcg
     5281 gggatgcgtg cgcagccgga cgtgggcctg cccagcgcgt cccgcgtgat catcggcgtg
     5341 gcgatcacct ttgcactggc ggtcggcgcg atcgtgctca tccgacgctt cgcgccgaat
     5401 gccgccacac gctggggcgg cggatccaca tcgtcgataa agttgctggc gcgcacccag
     5461 ctcagcagaa cgctgtgcgc gcaggtcgtg gaattcgaat cgacccgaat actgatcgtc
     5521 gaggcgcgcg agggtgtcgg gctgacgctg ttgcccaaat ccgaaacgcc cgggcgaccc
     5581 ccttccccat gacgggcgca gcgcagcgtg cagcaggctt tcagcgcgcg gcggcgcggc
     5641 gcagatatgg cgtgttgctt gcaagcgtca tcggcatgct catggcgagt gctttgatgt
     5701 ccaccgccct cgcagccgct ccgaattcat tgggactgag tatcgaggtt ggaaaacccg
     5761 gcgcggttcc cgtggccgat gcgctgaaga tcatcctgat gctcaccgcc ctggcactgg
     5821 cgcccggcat cctgatcgcg atgacctcgt ttacccgcac gatcatcgtg ctgtcgatga
     5881 ttcgccacgg ctttggcatg caggatacgc caccgaatct ggtgctgctc agc
//
LOCUS       DATMRM010000324         6391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3901202, whole genome shotgun sequence.
ACCESSION   DATMRM010000324 DATMRM010000000
VERSION     DATMRM010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3901202"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VNZ06_09700"
     CDS             <1..1011
                     /locus_tag="VNZ06_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWW21066.1"
                     /translation="VARGSFFSILGPSGCGKTTLLRMIAGFTPPDSGTIHIGGRNMHQ
                     VAPNRRPVNMVFQQLALFPMMSVGENVAFGLARRGVKRAERTRRAEAILERVGLAGAS
                     RKRIEQLSGGQRQRVAIARSLVLEPTLLLLDEPLGALDLKLREHMKIELKQLQAAFGT
                     TFVYITHDQSEALVLSDQVAVMNRGRFEQIGTPQQLYYRPGSAFVAGFVGANNRIDGH
                     VTAQDGELAELTTAQGWVIQAQRSGSLSTGSAAAVFVRPEAATLERSSGQLPAQSLKF
                     GARVQSLLFDGANSAVLLREERSGTEFRVALAQTGRFADLQAGEPVLFGFEPQRAICF
                     QT"
     gene            1022..1882
                     /locus_tag="VNZ06_09705"
     CDS             1022..1882
                     /locus_tag="VNZ06_09705"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWW21067.1"
                     /translation="MPKRTLILVLLLAPALLWLLLLIIVPHLELALLSLRERVAPHEY
                     RASLMQYQTFFREPLYWQVFVRTAIVSAVATALTLLVAFPIAWVITKLAGARAGGVLF
                     LVCLIPFWVSETVRSLGWLILLRESGVLPALLVRLGLTPVPVEMLYHDATILVGLVYT
                     SLLFMVVPLVNTLESLDNALIEAAYNLGASGFSILRRIVIPHAAPGITAGCIVVFMLT
                     LGNYLTPTLLGGKNSQWFTELIYTQFIVRFNWEQGAAFGFLLLALSTAIVALGLRLSG
                     QRFVDVMRRA"
     gene            1879..2736
                     /locus_tag="VNZ06_09710"
     CDS             1879..2736
                     /locus_tag="VNZ06_09710"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWW21068.1"
                     /translation="MIASLPRPLWLRTTTMLYIGAFLIFLFLPLLTVAVFAFNDADYP
                     APPWHGFTLDWFFGNGSRGRVGLFHDSELLGSIWTSVVVATWVTGLSVLIGSANAFLM
                     ERYRFPGKQALTVLMLLPLVVPGVILGISILAFFSRIAQLGDALFGWELDFLRPGLPL
                     VVLGQFSYIVTIATLLISARLKRFDLSLEEAALNLGASHAAVLRTITLPYLRPALLGS
                     AAISFLMSFENFNTTLMLVGADAPLTVAMYGRMREGASPVLNAVSLFLMTASALLALA
                     VMRRERQPA"
     gene            2823..3674
                     /locus_tag="VNZ06_09715"
     CDS             2823..3674
                     /locus_tag="VNZ06_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21069.1"
                     /translation="MSDAAQQMEAPSQPTQAPPPSAEPTPAAPPKTVTLANFLENSVV
                     LASATVSIECEYIGARYDLVVPDVHLFCNSPLCGKVMAFAPDAARWTVPDRTSWIELK
                     FLRYTCRSCQKSPKLYAVRIRFSNESLNEAAFTKIGEEPPNIGPTPKSLRDLLGDAWV
                     LYLKGVRAEMAGLELGAFVYYRRAVERIWPALLDKFIKLSQYDASLDQLRQLQDAREA
                     KPINRSMEGAKESIPTTLYVDGKNPLEALIEAYGDRTKEYPSAECTARAEGMRRALNQ
                     LASIGSG"
     gene            complement(3691..5598)
                     /locus_tag="VNZ06_09720"
     CDS             complement(3691..5598)
                     /locus_tag="VNZ06_09720"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21070.1"
                     /translation="MKRFPAVLAIFAAAISFTSLPGVAAAPIPAPEDRPYPGEIHLSV
                     DASDLQHRIVHVHESITGLGADAVLLFPKWLPGSHGPSGPLDRLAGLTIAANGNPLSW
                     TRDTIDVFAFHVPVPAGSSSVELDFQYLSGVAARGRPEISQNVMILNWNALILYPAGY
                     YSRQIPVQASLTLPEGWKLGSALEPLADAGTQTTFKTVTLNTLVDSPVYAGRYTAQLD
                     LDPGGVVPVHLDLFADRAEALYVRPEQLAAHRALVQQAYKLFGSHHYSHYDFLYSLSE
                     EVLQKGLEHHQSSENGANPDLFSAWEKTAWARDLLPHEFTHSWNGKFRRPADLWTPNF
                     NVPMQDSLLWVYEGQTQYWGSVLAGRSGLRTRQQALDQLAMTAAFYESQTGREWRPLQ
                     DTTNDPIFQMRRPEPWRDWQRSEDYYHEGLLIWLDADTLIRERSRGRRSLDDFARAFF
                     GVQDGSYVTLTYTFDDVVAALNAVEPYDWAKFLHERTDSVGKPAPLDGLRRGGYHLVY
                     TETPSEYEKQSEDGRHRNNQTYSIGLEIDQKDSVIATVIWDSPAFRANLLDGDAILAV
                     NGNAYNAEALSDAIRAAKGTPAKIELIVRNGGHYKIAVIDYHGGLRYPHLERDAAAAP
                     ALLDEILAPRR"
     gene            complement(5655..6179)
                     /locus_tag="VNZ06_09725"
     CDS             complement(5655..6179)
                     /locus_tag="VNZ06_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWW21071.1"
                     /translation="MRTLATVAVTLVSSLAALGAGAAVFVTSGAYNIGADDHHTTAVF
                     AMIEELRQRSIDVRSRTLAVPPNLDDDARIREGAAYYAAHCPLCHLAPGVDRDKADVR
                     RGMYPHPPNLSQEGVNDPGQAFWIVKHGIKMSAMPSWRKTLDDEDIWDVVAFLRKVPG
                     MTAERYQQLSGGDH"
     gene            complement(6185..>6391)
                     /locus_tag="VNZ06_09730"
     CDS             complement(6185..>6391)
                     /locus_tag="VNZ06_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HWW21072.1"
                     /translation="TSVKVKAGTRITWMNKDEEPHTIASDEGLFRSSALDTDESFSHE
                     FDKPGTYHYTCTIHPRMIGTITVE"
BASE COUNT          993 a         2070 c         2077 g         1251 t
ORIGIN      
        1 gtcgcgcgcg gcagcttctt ttccattctg ggaccttcgg gctgcggcaa gaccacgctg
       61 ctgcggatga tcgccggctt cacgccgccg gacagcggca cgatccacat cggcggacgc
      121 aacatgcatc aggtggcgcc caaccgccgg ccggtgaata tggtgttcca gcagctcgcg
      181 ctgttcccga tgatgtcggt cggcgagaac gtcgcatttg gtctggcccg tcggggcgtg
      241 aagcgcgccg agcgcacgcg gcgcgccgag gcgatcctcg agcgcgtcgg actggccggt
      301 gcgtcgcgca agcgcatcga gcagctctcc ggcggacagc gccagcgcgt cgcgatcgcg
      361 cgcagcctgg tcctggagcc gacgctgctg ctgctggatg agccgctcgg cgcgctggat
      421 ctgaagctgc gcgagcacat gaaaatcgag ctgaaacagt tgcaggccgc gttcggcacg
      481 accttcgtct atatcacgca cgatcagtcc gaggcgctcg tgctgtccga tcaggtcgcg
      541 gtgatgaatc gcggccgctt cgagcagatc ggcacgccgc agcagctcta ctaccggccg
      601 ggcagcgcat tcgtggccgg attcgtcggt gccaacaatc gcatcgacgg gcacgtgacc
      661 gcacaggacg gtgagctcgc cgaactcacc accgcgcagg gctgggtgat ccaagctcag
      721 cgcagcggca gcctcagcac cggcagtgcg gcggcggtgt tcgtgcgacc ggaggcggcc
      781 acactggaac gcagcagcgg gcagctgccg gcgcagtcgc tgaagttcgg cgcccgggtg
      841 cagagcctgc tgttcgacgg cgcgaactcc gcggtgctgc tgcgcgaaga gcgcagcggc
      901 accgagttcc gcgtcgcgct ggcgcagacc gggcgcttcg ccgatctgca ggccggagag
      961 ccggtgctgt ttggttttga gccgcagcgt gcgatctgtt ttcagacctg acgctcgagc
     1021 gatgccgaaa cgcacactga ttcttgtcct gctgctggcg ccggcactgc tgtggctgct
     1081 gctgctgatc atcgtgccgc acctggagct ggcgctgctg tcgttgcgcg agcgcgtggc
     1141 tccccacgag tatcgggcct cgctgatgca gtaccagacc ttctttcgcg agccgctgta
     1201 ctggcaggta ttcgtgcgca ccgcgatcgt gtccgcggtc gcgaccgcat tgaccctgct
     1261 ggtggccttt cccattgcct gggtcattac caagcttgcg ggagcgcgcg ccggcggtgt
     1321 gctgttcctg gtgtgcctga tccccttctg ggtcagcgag acggtgcgct cactgggctg
     1381 gctgattctg ctgcgtgagt cgggtgtgct gccggcgttg ctcgtgaggt tgggcctcac
     1441 gcccgtaccg gtggagatgc tctatcacga cgcgaccatt ctggtcggac tggtctatac
     1501 ctcgctgctg ttcatggtgg tgccgctggt gaacactctg gagagcctcg acaacgcgct
     1561 gatcgaggct gcctacaacc tgggcgccag cgggttctcg atcctgcggc gcatcgtcat
     1621 tccgcacgcg gcgcccggca tcaccgccgg gtgcatcgtg gtgttcatgc tgacgctggg
     1681 caactatctg accccgacgc tgctcggcgg caagaactcg cagtggttca cggagctgat
     1741 ctacacgcag ttcatcgtgc gcttcaactg ggagcagggt gccgccttcg gcttcctgct
     1801 gctggcgctg tccaccgcaa tcgtcgcgct gggcctgcgg ctgtcgggac agcgctttgt
     1861 ggatgtgatg cggcgcgcat gatcgcctca ctgccgcgtc cgctgtggct gcgcaccacg
     1921 acgatgctct acatcggcgc atttctgatc ttcctgttcc tgccgttgct gacggtggcg
     1981 gtgtttgcgt tcaacgacgc cgactatccg gcccctccct ggcacggctt cacgctcgac
     2041 tggttcttcg gcaacgggtc gcgcggacgg gtcggactgt tccacgacag cgagctgctc
     2101 ggcagtatct ggaccagtgt cgtggtcgcc acctgggtga cggggctgtc ggtgctcatc
     2161 ggcagtgcga acgcattcct gatggaacgc taccgcttcc cgggcaagca ggcgctcacc
     2221 gtgctgatgc tgctgccgct ggtcgtcccg ggtgtgattc tcggcatctc gatcctggcc
     2281 ttcttcagcc gcatcgcgca gctgggcgat gcgctgttcg gctgggagct ggactttctg
     2341 cggcccggac tgcccctggt ggtgctcggc cagttctcct acatcgtgac gatcgccaca
     2401 cttctgatca gcgcgcggct caagcgtttt gacctctccc tggaggaggc ggccctgaac
     2461 cttggcgcat cgcacgcggc ggtcctgcgc acgatcacgt tgccgtatct gcgcccggcg
     2521 ctgctcggat cggcggcaat ttctttcctc atgtcgttcg agaacttcaa taccaccctg
     2581 atgctggtcg gggcggatgc gcccttgacg gtggcgatgt atggtcgcat gcgggagggt
     2641 gccagcccgg tcctgaacgc ggtcagcctg ttcctgatga ccgcctcggc gctgctggcc
     2701 cttgcggtca tgcggcgtga gcggcagcca gcgtgaccgt tgtccgctgt ggggccggtt
     2761 cttgttagta ttctgcggaa tggatgctgg ttcaggacgc cgcccgaccc taacatgccg
     2821 ccatgagtga cgctgcccaa caaatggagg ccccttcgca accaacgcag gcgcctcccc
     2881 cgtctgccga accgaccccg gcggcccctc cgaagacggt gacgctggcg aacttcctgg
     2941 agaactccgt ggttctggcc agcgccacgg tgtcgattga gtgcgagtac atcggtgccc
     3001 gctacgacct ggtggtcccg gatgtgcatc tgttctgcaa ttccccgttg tgcggcaaag
     3061 tcatggcctt tgccccggac gcggcccgct ggaccgtgcc ggaccgcacc agctggatcg
     3121 agctgaagtt cctgcgctac acctgccgca gctgtcagaa gtccccgaag ctctacgcgg
     3181 tgcgcatccg cttcagcaac gagtcgctca acgaggccgc attcaccaag attggcgagg
     3241 agccgcccaa tattggtccg accccgaagt cgctgcgcga tctgctgggt gatgcgtggg
     3301 tgctctatct caagggggtg cgcgcagaga tggcgggcct ggagctgggc gctttcgttt
     3361 attaccggcg cgcggtcgag cgtatctggc ctgcgctgct ggacaagttc atcaagctct
     3421 cccagtacga cgcatcgctc gatcaactgc gtcagctgca ggatgccagg gaagccaagc
     3481 ccatcaaccg ctcgatggaa ggggcgaagg aatcgatccc gaccacgctc tatgtggatg
     3541 gcaagaatcc gctcgaggcg ctgatcgagg cctacggcga tcgcaccaag gaataccctt
     3601 ccgccgaatg caccgcacgc gccgagggca tgcgtcgcgc gctcaatcag ctcgccagca
     3661 tcggcagcgg ctgatggtcg gcgccgcgtg ctatctgcgc ggcgccagga tctcgtccag
     3721 cagcgcgggt gccgcggcgg catcgcgttc cagatgcggg tagcgcagtc cgccgtgata
     3781 gtcgatcacg gcgatcttgt agtgtccgcc gttgcgcacg atcagctcga tcttggccgg
     3841 cgtgcccttg gcggccctga tcgcatcgct cagggcttcc gcgttgtagg cattgccgtt
     3901 gaccgcaaga atcgcgtcgc cgtccagcag gttcgccctg aacgccggac tgtcccagat
     3961 gacggtggca atgacgctgt ctttctggtc gatctccagc ccgatcgagt aggtctggtt
     4021 gttgcggtgg cggccatcct cactctgctt ttcgtactcg ctgggcgtct cggtgtacac
     4081 caggtgatag ccaccgcgac gcagcccatc cagcggcgcc ggtttgccga cgctgtcggt
     4141 gcgctcgtgc aggaacttcg cccagtcgta gggttccacc gcattcagcg ccgcaaccac
     4201 gtcatcgaag gtgtaggtga gggtcacata gctgccgtcc tggacgccga agaacgcccg
     4261 cgcgaagtca tccagcgagc gcctgcccct ggagcgttca cggatcagcg tgtcggcgtc
     4321 gagccagatc agcagtccct cgtggtagta gtcctcggaa cgctgccagt cgcgccacgg
     4381 ttccggccgg cgcatctgaa agatcggatc gttggtcgtg tcctgcaggg gacgccactc
     4441 acgcccggtc tgtgactcgt agaatgccgc ggtcatcgcc agctgatcga gcgcctgttg
     4501 ccgtgtgcgc agccccgagc gcccggccag cacgctgccc cagtattgcg tctgtccctc
     4561 gtagacccac agcaggctgt cctgcatcgg cacattgaaa ttcggcgtcc acaggtccgc
     4621 cgggcggcgg aatttgccgt tccaggagtg cgtgaattcg tgcggcagca gatcgcgtgc
     4681 ccacgcggtc ttctcccacg cgctgaacag atccggattg gcgccattct ccgaggactg
     4741 gtgatgctcc agacctttct gcagcacttc ctcgctcaac gaatacaaaa aatcgtagtg
     4801 cgagtagtga tgcgagccga acagtttgta cgcctgctgc accagcgcgc ggtgcgccgc
     4861 gagttgctcg ggtctcacgt acagcgcctc ggctctgtcc gcgaacaggt ccaggtgcac
     4921 cgggaccacg ccgcccggat ccaggtccag ttgcgccgtg tagcgcccgg cgtagaccgg
     4981 actgtccacc agggtgttga gcgtgacggt tttgaacgtg gtttgcgtgc cggcgtcggc
     5041 cagcggctcg agcgccgagc cgagcttcca gccctcgggc agcgtcagcg aggcctgcac
     5101 cggaatctga cgcgagtagt agcccgccgg atacaggatc agggcgttcc agttgaggat
     5161 catcacgttc tggctgatct cgggtcgccc acgagcggcg acgcccgaca ggtactgaaa
     5221 gtccagctcc accgatgagg atccggccgg aacgggcacg tggaaggcga acacatcgat
     5281 cgtatcgcgc gtccagctca gcggattgcc gttcgcagcg atcgtcagac ccgcgagccg
     5341 gtccagagga ccgctcggtc cgtggctgcc tggcaaccac ttcgggaaca gcagtaccgc
     5401 gtcagctccc agtccggtga tgctctcgtg cacgtgcaca atgcgatgct gcagatcgct
     5461 cgcgtccacg ctcagatgta tttcacccgg gtagggcctg tcttcggggg cggggatcgg
     5521 cgctgcggcc acaccgggga gggaggtaaa gctgatggct gcggcaaaga tcgccaggac
     5581 ggcgggaaat cgcttcacgg ccgacacacc tcgttgctct tgttctgtgc cgccttagta
     5641 taaagcctga gtcgtcagtg gtccccgcct gacaactgtt ggtatcgctc ggccgtcatc
     5701 ccgggcacct tgcgcaggaa cgcgaccaca tcccagatgt cctcgtcgtc cagcgttttg
     5761 cgccaggagg gcatcgcgct catcttgatg ccgtgcttga cgatccagaa tgcctgccca
     5821 ggatcgttta ccccttcctg cgacagattc ggcggatgag ggtacatgcc gcgacgtacg
     5881 tcggctttgt cacggtccac gccgggcgcc agatgacaca gcgggcagtg cgcggcgtag
     5941 taggcggccc cctcccggat gcgggcgtcg tcgtccagat tcggcggcac cgccagcgtg
     6001 cgcgagcgca cgtcgatcga gcgctgccgc agctcttcga tcatcgcgaa caccgctgtg
     6061 gtgtgatgat catcggcacc gatgttgtac gcgccagaag tcacaaacac ggcggctccg
     6121 gctcccaatg cggccagcga gctgaccagc gtgaccgcga ctgtggcgag tgtgcgcatg
     6181 ccgcttactc aaccgtgatc gttccgatca tgcgcggatg gatcgtgcag gtgtagtgat
     6241 aggtacccgg cttgtcgaac tcgtgtgaga acgactcgtc ggtatccagc gcggacgatc
     6301 ggaacaagcc ctcgtcgctg gcgatcgtgt gcggctcctc atccttgttc atccaggtga
     6361 tcctggtgcc ggctttcacc ttcacgctcg t
//
LOCUS       DATMRM010000325         4355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_818555, whole genome shotgun sequence.
ACCESSION   DATMRM010000325 DATMRM010000000
VERSION     DATMRM010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818555"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3162)
                     /locus_tag="VNZ06_09735"
     CDS             complement(<1..3162)
                     /locus_tag="VNZ06_09735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW21073.1"
                     /translation="MSDLHFLARGGEMGTRMRAFDWSRTPLGAPQFWPQSLKTIVRMM
                     LDSRYAMWMAWGPELTFFCNDAYLPTVGIKRDWCLGSRSELVWQEIWPDIGPRIQHVL
                     SSGEATWDEALQLFLERSGFREETYHTFSYSPVYDDDSAIAGMLCVVTEVTDRVIGER
                     RLHALRDLAARSTGVQSVEDACRRVASVLRQDPFDLPYAGLYLVNSDRSKARLVASTR
                     ELSESQVPRQYLLHDPAVPSAMKELMLTGQRRRIDDMAGARLCISSAWPEPVEQAQLL
                     PIKLAGHERLEGFVLLGISPRRALDDAYVDFLDLIANQIASTISDAQSYEAERARAES
                     LAQLDRAKTAFFSNVSHEFRTPLTLIMSPLEQALRAPARDLSPALREEMLIAHRNSLR
                     LQRLVNALLDFSRMEAGGMRASFEPSNMAALSAQLAGTFRTTVERAGLTLQVDCPELP
                     QPVYLDRDLWEQILLNLLSNAFKYTLCGSIRVEVRSTATHAVLSVCDTGTGIPPDAVP
                     HLFERFYRVPNAHGRTFEGTGIGLALVHELVALHGGQIEVQTEVGKGSTFTILLPYGS
                     VHLPAQSLHRSSPSDKGKASASALVEEAMSWRMPESGHAEASLPLSPSGASEGTILLV
                     DDNADMRSYLQRLLQPHWDIVTAADGLQALERLKGIKPDLVLSDVMMPRLDGFGLLAA
                     IRANEPISDVPVVLLSARAGEEARVEGINASANDYLVKPFTARELVARVRAQIGIARL
                     QREARTAIQSAERASRTAVEALNRQLTLESEELRNLFEQAPSFTAVLKGPELIVAMSN
                     RACQTLLGGRSLLGQPLFEAVPELTAEPFPELLRQAFESGEPYVSRALPVKLQQLPGG
                     RLEELFLDLVFQPIKDSVGGVTGILVQGNDVTEHKRMENALRVSDRRKDEFLAMLAHE
                     LRNPLAPILNATELLSRSARDSEQTGAIAQLIRRQAAQMARLVDDLLDVSRITQGRIE
                     LRREVVEIGGIVSQALETVYPLLNERQHHVSVTTSYRPLYVDGDVTRLAQCIGNVLTN
                     AAKYTDRGGQIRLDIREQREEVVI"
     gene            complement(3331..4197)
                     /locus_tag="VNZ06_09740"
     CDS             complement(3331..4197)
                     /locus_tag="VNZ06_09740"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF042973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-polyphosphate phosphotransferase"
                     /protein_id="HWW21074.1"
                     /translation="MKIRSQDYRVSEGHRVRLRKWPTQVEPAYQSKQHYETLLARHVE
                     ELSSLQQLFYACNRYAVLLIFQAMDAAGKDGVIKHVMSGVNPQGCQVFSFKHPSATEL
                     QHDFLWRTTRDLPERGRIGIFNRSYYEEVLIVRVHPEILQSQGLPPGAAADPRIWEHR
                     YRSIVDFERHLYANDTRIVKFFLHLSRDEQRQRFVARIDEPQKNWKFSMADIEERGYW
                     KQYMQAYEQCLSATSTRRSPWYVVPADDKRNARLIVSSIVLDTFKALDMEYPRISAQR
                     LRELRAIRKLLI"
BASE COUNT          824 a         1421 c         1310 g          800 t
ORIGIN      
        1 aatcaccacc tcctcgcgct gctcgcggat gtcgagccga atctgtccgc cgcgatcggt
       61 gtacttggcg gcattggtga gaacgttgcc gatacattgc gccaaacgcg taacatcccc
      121 gtccacgtac agcggacgat agctggtggt caccgacacg tgatgctgcc gctcgttcag
      181 caacggatac acggtctcga gcgcctgact gacgatgccg ccgatctcca cgacctcgcg
      241 ccgcagttca atacgtccct gcgtgatccg cgaaacatcc agcagatcat ccaccagtcg
      301 cgccatctgc gcggcttggc gccggatcaa ctgcgcgatc gccccggtct gctcgctgtc
      361 gcgcgcgctg cgtgataaca attccgtggc gttgagaatg ggcgccagcg gattgcgcag
      421 ctcgtgcgcc agcatcgcca gaaactcatc cttgcgccgg tcggagacgc gcagcgcgtt
      481 ctccatgcgt ttgtgctcgg tgacatcgtt cccctgaacc aaaatgcctg tcacgccgcc
      541 gacgctgtcc ttgatgggct ggaagaccag atcgaggaat aactcctcga gccgaccgcc
      601 ggggagctgt tgcaatttca ccggcagagc ccggctgaca tacggctcgc cgctttcgaa
      661 cgcctggcgc agcagttccg gaaacggctc ggcagtcagc tcgggtaccg cctcgaacaa
      721 cggctgtccg agcagtgagc gcccgccgag cagagtttga caggcgcggt tgctcatcgc
      781 gacgatcagc tccggaccct tcagcacggc tgtaaagctc ggcgcctgct cgaacaggtt
      841 gcgcaattct tcgctctcga gggttagctg ccggttcaag gcttccactg ccgtgcggct
      901 cgcccgctcc gcgctctgta tcgcggttct ggcctcgcgc tgcaggcgcg cgatgccgat
      961 ctgcgctcgc acccgggcaa ccagttcccg cgccgtaaac ggcttcacca gatagtcgtt
     1021 ggcgctggca ttgatgccct cgacgcgggc ctcctcgccg gcgcgcgccg acagcagcac
     1081 cactgggaca tcgctgatcg gttcgttggc gcggatggcg gcgagcagtc cgaagccgtc
     1141 caggcggggc atcatcacat cgctcaacac cagatcgggc ttgatgccct tcagacgctc
     1201 caacgcctgt aagccatcgg cggcagtgac gatgtcccaa tgaggctgca gcaggcgctg
     1261 cagataggaa cgcatatcgg cattgtcatc caccagcagg atcgtgccct cgctggcccc
     1321 gctcggtgac aacggcaagc tcgcctccgc atggccgctt tcgggcatcc gccaggacat
     1381 cgcctcttcc accagtgcgc tcgcactggc tttgcccttg tccgaagggc tcgagcggtg
     1441 cagcgactgc gccggcaggt gcaccgaacc gtatggcaac aggatcgtga aggtggaccc
     1501 cttaccgact tccgtctgca cttcgatttg gccgccatgc agggcaacca gctcatgcac
     1561 cagcgccaga ccgattccgg tgccctcgaa ggtgcgcccg tgcgcattag gcacgcgata
     1621 gaagcgctcg aacaggtgcg gtaccgcgtc cggcggaatg ccggtaccgg tatcgcagac
     1681 actgagaacg gcatgcgttg cggttgaacg cacctcaacc cggatcgaac cgcagagcgt
     1741 gtatttgaaa gcattggaca gcagattcag caggatctgt tcccacaagt cccgatcaag
     1801 atagaccggc tgcggcagct cggggcaatc gacctgcagc gtcaggccgg cacgctcaac
     1861 cgtggtacga aatgtcccgg ccagttgggc tgacagcgcc gccatattcg acggttcgaa
     1921 actggcgcgc atcccgccgg cttccatgcg cgagaaatcc agcagcgcat tcaccaatcg
     1981 ctgcaaccgc agactgttgc gatgagcaat gagcatttct tcgcgcagcg ccggggacaa
     2041 atcacgcgcc ggcgcgcgca gcgcctgctc cagcggactc atgatcaatg tcagcggcgt
     2101 acgaaactca tggctgacat tggagaagaa cgccgtcttg gcgcggtcca gttgcgccag
     2161 gctctcggcg cgcgcccgtt cggcctcata ggactgcgca tccgaaatcg tcgaggcaat
     2221 ctgattggca atcagatcga ggaagtcgac ataggcatcg tccagcgcgc ggcgcgggct
     2281 gatcccgagc agcacgaatc cctccagtcg ttcgtgacca gcaagcttga tcggcaatag
     2341 ctgcgcctgc tcgaccggct ctggccaagc ggacgaaatg cacaggcgag cgcccgccat
     2401 gtcatcgatg cgccgacgct gacccgtgag catcagctcc ttcatggccg acggcaccgc
     2461 gggatcgtgc aacaggtact gccggggtac ctgcgactcg gaaagctcgc gggttgacgc
     2521 caccagccgc gctttgctgc gatcgctgtt cacaagatag aggccggcgt agggcaggtc
     2581 gaacggatct tgcctcagca ccgacgccac gcgccggcag gcatcctcca ccgattggac
     2641 acccgtcgaa cgtgcggcaa ggtcgcgcag cgcgtgcaga cggcgctccc cgatcacccg
     2701 atcggtcact tcggtgacga cgcacagcat gccggcaatg gcgctgtcat cgtcatagac
     2761 cgggctgtag gaaaacgtgt gataggtttc ttcgcgaaag ccactgcgtt cgagaaacag
     2821 ctgcagggct tcgtcccagg ttgcctcgcc gctgctcaac acatgctgga tgcgcgggcc
     2881 gatatccggc cagatctcct gccacaccag ctctgagcgt gaacccagac accaatcgcg
     2941 cttgataccg acggtcggca gataggcatc attgcagaag aaagtaagct caggtcccca
     3001 cgccatccac atcgcgtagc gcgagtcgag catcatgcgc acgatggtct tgagcgattg
     3061 cggccagaac tgcggtgctc cgagcggcgt gcgtgaccag tcgaaggcgc gcatccgcgt
     3121 gcccatctcg ccgccacgcg cgaggaaatg cagatcggac acttcgttcc gaaggcgcgg
     3181 gagcgagctg gccaaagcga tccgacctcc aactccgagg ccccaccgtg ttttgcagtt
     3241 gtagccggct gcggagtgtg gcgattgcac agcatccaac atcagcgggg ctgtaggacg
     3301 gccggagggc cggctgtcgg aaggtaccct ctagatcaga agctttcgaa tcgctcgcag
     3361 ttctcgcagg cgttgcgcgc tgattcgcgg atattccatg tccagggcct tgaaggtatc
     3421 cagaacaata ctcgacacga tcagacgggc gtttcgctta tcatccgccg gtaccacata
     3481 ccagggtgag cgccgcgtac tggtggcgct gaggcactgc tcataggcct gcatatactg
     3541 cttccagtac ccgcgctcct cgatgtcggc catgctgaac ttccagttct tctgcggttc
     3601 gtcgatgcgc gccacaaagc gttggcgctg ctcgtccctg gacagatgca ggaagaactt
     3661 aacgatacgt gtgtcgttgg catacaagtg ccgttcgaag tccacgatcg agcgatagcg
     3721 atgttcccag atccggggat ctgcggctgc acccggtggc agcccctggc tctgcagaat
     3781 ctcgggatgg acgcggacga tcagcacttc ctcgtagtag gaccggttga agattccaat
     3841 tcggccgcgc tcgggcaaat cgcgcgtcgt ccgccacagg aagtcgtgct gcagttccgt
     3901 ggcggagggg tgcttgaagc tgaacacctg gcagccctgc gggttcacgc cggacatcac
     3961 gtgcttgatg acaccgtcct tgccggccgc gtccatcgcc tgaaagatca gcagcaccgc
     4021 atagcggttg caggcgtaaa acagctgctg cagagaactc agctcttcga cgtgccgcgc
     4081 gagcagcgtc tcgtagtgct gcttggactg ataggctggc tcgacctgtg tcggccactt
     4141 gcgcaatcgg acccggtgtc cttcagatac gcggtagtcc tgcgatctga tcttcatcgg
     4201 cgccgtgacg ctctagtgcc ccgtaccatc acctttgcgg ccggcttcgt acaggaacca
     4261 ggtgcgacgc tcggtctcgt cgatccatac ctcgatcagg ctggcgctcg caatgtcccc
     4321 gccctcgtcc accacgccgt gcacttcacg caggc
//
LOCUS       DATMRM010000326         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2354919, whole genome shotgun sequence.
ACCESSION   DATMRM010000326 DATMRM010000000
VERSION     DATMRM010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2354919"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..147
                     /locus_tag="VNZ06_09745"
     CDS             <1..147
                     /locus_tag="VNZ06_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003089113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HWW21075.1"
                     /translation="DACQFYYECIGCYALLRPLAGHCCVFCSFGTVVCPPKQMQRACY
                     SADD"
     gene            1153..2268
                     /locus_tag="VNZ06_09750"
     CDS             1153..2268
                     /locus_tag="VNZ06_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWW21076.1"
                     /translation="MPDLPVGFEVRIAGGAFLLVSCGLMLLGLLARSIRPRALSDRIA
                     AAALFTAGLFSSLLVFTLLRDVVLWAAWLPLSAEHLQMLETSSAVLVVGLTVFATVLG
                     FANARRRARIKRVEIPLRNLPHALHGFSIAQISDIHVGPTIKRGYVDAIVDAVNALDA
                     DLIAVTGDVVDGSVQDLAPHTEPLARLRARHGAFFVTGNHEYYSGEQAWTAEFKRLGL
                     RVLLNEHVIVRHRGAPVVIAGVTDYSSHHFNTTQRSDPAAALVGAPSNAAAKILLAHQ
                     PRSAAAAATAGFDLQLSGHTHGGQFWPWTLFVRFQQPFTAGLHRLNSLWVYISRGTGY
                     WGPPNRFGAPSEITLLRLVPAKPADTLGTLEPLCRAL"
BASE COUNT          406 a          758 c          701 g          542 t
ORIGIN      
        1 gatgcctgcc agttctacta cgagtgcatc ggatgctacg cactgttgcg cccgctcgcc
       61 ggtcactgct gtgtcttctg ctctttcggc acggttgtct gccccccgaa gcagatgcag
      121 cgggcgtgtt atagcgccga cgactagcta agtatcacgc cgatggcaga gatcttcgcg
      181 cgctgcgaag gcttcggtgg caatacgttg cgccgccagc cccaagtcgc tgcgcccctg
      241 ttccctagtg ctgactctca agtatccgat tgccgcttgc atcgtgaggt cccccgtaga
      301 tcgcctccac gatatgccca tcaacgttcg ctcgccgctc ccatctcaca ttgaacggct
      361 gactgcgggc caactgcagc cggtgaggat gccgaatggc ctgctccgaa gcggacacgc
      421 cggttaacgg tgtatcctct attgcacgca gcgacagacc agcggggtgc aacgggtctt
      481 cgcccactgg tggtcgttag cggggtatga cgaagtgagg ctcagccaag gcatgctgcg
      541 caaacagata ctcgtaacga tctgcgcgac ggcggcggta gttacattgc taatgttgtg
      601 gctgcatttg gcggagctac aactcccctt gcccgacggc gacgaccaag tctcgcggct
      661 cgcgtttgcg gcacgatggc tgcttcttcc ggggttagct ctcttcgccg gaattgctgt
      721 agtggctaaa ttgcgattct ttgtacccga cgcaatcgat tctttgtacc cgacgcaata
      781 gatggcgcgc ccgcctcgcg gacgtgaagg agggattcga tccatcttgc ccctgcgatg
      841 ctcgtcgcgg agcttccaac aagtgattga aattagagac tctaaaatcc aaagggtgcg
      901 ctcataatgc cgaggtcgtg ggttcgagtc ccacccaagc caccaatcga tcaattactt
      961 gcagccgggc tgggctcgcg aaagcctcgc gcgctccgtt gaagtcttgg attccagtcc
     1021 catatcgcct tcttgaatcg tcttcaaccc gtaccagcgc catacccgac cagccgaatg
     1081 tcactccgcc gctccttgct ccttccagtg agcctcttag gacttcttca cgcttatatc
     1141 ggctggcggg tgttgcctga cctgccagtg ggcttcgagg tccggatcgc cggcggcgcc
     1201 ttcctgctcg tctcgtgcgg gttgatgctg ctgggtcttc tggcgcggtc gattcggccc
     1261 agggcattgt cggatcgcat cgccgcggcg gcgttgttca cggcgggcct cttttcctct
     1321 ctgctcgtgt tcaccctgct gcgcgatgtc gtgctgtggg cggcctggct gcctttgtcg
     1381 gcagaacatt tgcagatgct agaaaccagc tctgcagtgc tcgtggtagg cctcaccgtg
     1441 ttcgccaccg tgctcgggtt tgcaaacgcg cgccgccgtg cccgcatcaa gcgcgtcgaa
     1501 attccactgc gcaatttgcc acacgcgctg cacgggtttt cgattgcgca gatcagcgac
     1561 atccatgttg gtcccaccat caagcgcgga tacgtcgatg cgatcgtcga tgcggtaaat
     1621 gcactcgatg cagacctgat tgcggtgacc ggcgatgtgg tcgacggttc agtgcaggac
     1681 ctcgcgccgc acaccgagcc ccttgcgcga ttgagggcgc gccacggtgc atttttcgtg
     1741 acgggcaatc atgagtacta ctctggggag caggcctgga ccgccgagtt caagcggctg
     1801 ggattgcgcg tactgctcaa cgagcatgtc atcgtgcgac atcggggtgc tccagtggtc
     1861 atcgccggtg tgaccgacta cagctcgcat catttcaata ccacgcagcg cagtgatccg
     1921 gctgcggcgc tcgtgggcgc gccatcgaat gcggccgcga aaatcttgct agctcaccag
     1981 ccgcgctccg ctgccgcggc ggcgacggct ggtttcgatt tacagctgtc tggccatacc
     2041 catggcggcc agttctggcc ctggaccttg ttcgtgcgat tccagcagcc cttcacggcc
     2101 ggattgcacc gcctgaacag tctctgggtc tacatcagtc ggggaacggg ttactgggga
     2161 ccgcccaatc gctttggcgc accctcggaa atcactctac tgcggctggt accggcgaaa
     2221 ccggctgaca cgttgggcac acttgaacca ctctgccgcg ctctttgatg ggtgtttggg
     2281 cctttttgtc gaacgggctc cactccgttc gctcagacca cgcctgagcc tgcgcgtcgg
     2341 tcatgatctg cactcgcatc ttccgggtct tgaaatgaaa cttcacccat acgcgcacac
     2401 cggcagc
//
LOCUS       DATMRM010000327         7800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2973298, whole genome shotgun sequence.
ACCESSION   DATMRM010000327 DATMRM010000000
VERSION     DATMRM010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7800
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2973298"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VNZ06_09755"
     CDS             complement(<1..728)
                     /locus_tag="VNZ06_09755"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HWW21077.1"
                     /translation="MNTFNIATLAVEALLQEVDVWPKPGLVSPLDRGSHSDMNAGLLR
                     RSAICLLPYFEQLVAAGAHGVGMSVLRRIGVAAEAAMLQVTDGVNTHRGAIFSLGLLC
                     AAAGAAHATQGHGELGTIVRHRWGEAIGTPTTRQSTHGAEVWRRYGVGGARKEAAAGF
                     PHLYEIAWPALKQGRRLAGGDEAAARVQALFALMAALEDTNLLHRGGAPGLLFARELA
                     VQFIEQGGVGRGDWRERALRVHRL"
     gene            934..1746
                     /locus_tag="VNZ06_09760"
     CDS             934..1746
                     /locus_tag="VNZ06_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWW21078.1"
                     /translation="MDAKQYGPWALVIGGSEGVGAAFARKLAASGFKLVLIARKRGPL
                     EALAAQLRASSAEVRVLCADLAHPEALERARSVTDELEIGLLIYNAGANNTRGRFVEL
                     PKEVTQSVIAINVIGQADFARHYGALMCQRGRGGMILAGSLSGYMGSPTLAAYTASKA
                     FSRIFTEALWAECKAFGVDVLHLNIGFTATPAMARLGIDISVAEAPDKVAQEGLDNIA
                     NGPLWIVSTKGNIERARKLSVVDARAELVRSFAIPPREQMADLQRRQLQSSD"
     gene            1803..2225
                     /locus_tag="VNZ06_09765"
     CDS             1803..2225
                     /locus_tag="VNZ06_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWW21079.1"
                     /translation="MDADLLHVQPSAPEPIYRQIVEQLRRLIASGKLLPGVELPSVRE
                     IAGRHAINPMTVSRAYSLLEYEGLLERQRGKGMIVAATRRRPQSDDSRLELLQPRLQE
                     VARQARELGLPDRRVLERLQALLEEHDERIAHAVYKPR"
     gene            2188..3141
                     /locus_tag="VNZ06_09770"
     CDS             2188..3141
                     /locus_tag="VNZ06_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWW21080.1"
                     /translation="MSALLMPSTNLVEPAPLACAGVSKRFDGSTILSRLDLQIPAGAV
                     LGLIGRNGAGKTTLIRILMGLLAPDAGTATVLGEPALLMSDAAKTCLGYVPQTPDALK
                     WMRVGEMLDFIGRFYPRWDPDYVADSLLRWTISPEKPLAKLSPGERQRVALIRALALR
                     PRLLVLDEPAASLDPVARRDLLREIALRAGESGTTVLFSTHIVSDLERVASHVAFLHE
                     GSLVLKAPMDDLKETHTRLWLPPAGAAQLTGKLPGELRRRQLPDGALSIVLAREAGAA
                     WPAATALPGADMEVLGLEDLFVEITRGFQPDFAVGRQRLRL"
     gene            3138..4538
                     /locus_tag="VNZ06_09775"
     CDS             3138..4538
                     /locus_tag="VNZ06_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21081.1"
                     /translation="MNRDCVSTWRALLGGLRGGMDRQMVVIAIIFAAFCAAVAILFAT
                     AGLPQGKWIATSSLAIGSFALSHLCAQCLRRLVRGAQLSLLPGTGPYVRRAALTQWLI
                     IGLAPALLILTLGGSEIHFCAVALAALFGQVTGTYAALPGALLAATGAFFVVVFRRSI
                     VGLTQQSLWVVSVLLTVTLLSWLVWIWRAYLRSVPAETELPAGSRWGLPLSHLLRSPG
                     PAGRQQPLSPTMVMRTFMAAPYAPATAAQRALQVGLILVVMCVPAALAFALGRSHTLR
                     DLERLLHAAWAPTLWVSGLVIWGWLLRGQVTSFSRGSGNLDELALLPMLGSAAMRRRQ
                     LYQATLRPPILVLSMLFLVALVPVIGDADSFGIYTRIAYIFSLLPALTLLGWQLPGHP
                     TSRQAWMAAPVVLLGQSSTVFLFSHPVFNARELDLVRLICLTCWVLLGIAAVEVMLFK
                     SRRLAERPHPFVEIAS"
     gene            complement(4766..5437)
                     /gene="mdcG"
                     /locus_tag="VNZ06_09780"
     CDS             complement(4766..5437)
                     /gene="mdcG"
                     /locus_tag="VNZ06_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase
                     holo-[acyl-carrier-protein] synthase"
                     /protein_id="HWW21082.1"
                     /translation="MIESLQRHALLRVTTLGWQRALARSPALASRPYVPEWAGRGWPV
                     IVRRYLPEDAAGLIPVAIPLPPQAGKPGATLQLQPEEIHAPLPPVALSACLDAAPVAW
                     RETLHSLLASSERCGCAPAVFGSLLWQMLTGLTYLHERSDLDLLWRVTRHEQAEELVR
                     SIARCARKCPMRIDGELVLPDGAGVHWREWDGAAEVLVRTLHRVERRRTDQLFQESWR
                     AGQAV"
     gene            complement(5430..6161)
                     /locus_tag="VNZ06_09785"
     CDS             complement(5430..6161)
                     /locus_tag="VNZ06_09785"
                     /inference="COORDINATES: protein motif:HMM:NF018535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit gamma"
                     /protein_id="HWW21083.1"
                     /translation="MSLDELLRALFGHSFTVDERADGVLLGEARLAPAQALTLIGVVN
                     AMPLGVEGALTLAHRILAAVRSGQRQPILVLIDSGSQRMSRRDELLGLNEYLAHLAKC
                     LMLADRCGFPTISLLYGGGAAGAFVATALATRALVALPGANPAVMDLPSISRVTKLAL
                     QVLQRIGASTPVFAPGLTNMQLTGAITEVWDPHADLPGELSALLRRMSELPEDARDRA
                     GEQYGGRKLSARVAEAVRQQAGRRD"
     gene            complement(6148..7050)
                     /locus_tag="VNZ06_09790"
     CDS             complement(6148..7050)
                     /locus_tag="VNZ06_09790"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF005530.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HWW21084.1"
                     /translation="MLELAPSWYEQSARERIEALLDSASFRELLGPEQRAISPHLQAF
                     DLPPAFDDGMIVGSGRLEGRAVLIAAQEGRFMGGAIGEVHGAKLVGLLRAAAATPASV
                     LILFDTGGVRLQEANAGEVAVAEIMRAVLIARAAGVAVIGLVGGRAGCFGGGALIAAC
                     CSRLIVSEQGRIGVTGPEVIESNKGVEEFDSADRALVWRTFGGKHRRLIGSADLFVED
                     DAASFRKASLRLIDQPPPFDLVATEAEHARLARRLARAGDARDAQQMWTRLRVPDASK
                     VADMPTEEFIALVSGLPDVSHESR"
     gene            complement(7043..7360)
                     /gene="mdcC"
                     /locus_tag="VNZ06_09795"
     CDS             complement(7043..7360)
                     /gene="mdcC"
                     /locus_tag="VNZ06_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase acyl carrier protein"
                     /protein_id="HWW21085.1"
                     /translation="MESLKFQHQAARRLPGARRQALLGVVASGNLEALFERTLTGVEC
                     RIEVLTSISGYAEVWSAVIADFVERYSPGGLLISIHDGGARPDTVALRLAQGARVMER
                     EDA"
     gene            complement(7375..>7800)
                     /locus_tag="VNZ06_09800"
     CDS             complement(7375..>7800)
                     /locus_tag="VNZ06_09800"
                     /inference="COORDINATES: protein motif:HMM:NF028267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HWW21086.1"
                     /translation="RKLVVQLVETFGEKMAPTFVESLDALKFADRLKLDIAPIMIYGD
                     DVTHIVTEEGIANLLLCQDAPEREQAIRGVAGYTAVGRARDRAMVTRLRERGVIRRPE
                     DLGIDPLDADRRLLAARSVRDLVRWSGGLYRPPARFRNW"
BASE COUNT         1349 a         2561 c         2478 g         1412 t
ORIGIN      
        1 atcaggcgat gcacgcgcag cgcgcgctcg cgccagtcgc cgcgtccgac gccaccctgt
       61 tcgatgaact gcactgccag ctcgcgagcg aacagcagcc ccggggcgcc gccgcgatga
      121 agcagattgg tgtcctcgag cgcagccatc aacgcaaaca gggcctgcac gcgcgccgcg
      181 gcttcatcgc cgccggctag ccgccgcccc tgcttgagcg ccggccacgc aatctcatac
      241 agatgaggaa atccggctgc cgcttccttc cgggcgccgc ccacgccgta gcgccgccac
      301 acctcggcgc cgtgtgtgct ctggcgtgtc gtcggggtcc cgatcgcttc accccatcga
      361 tgtcgcacga tcgtacccag ctccccatgg ccctgcgtcg cgtgggcggc gccggccgcg
      421 gcgcacagca gacccagact gaagatcgcg ccgcgatgtg tgttgacgcc atcggtgacc
      481 tgcaacatcg cggcctctgc agccacgccg atccgccgca gcaccgacat gccgacaccg
      541 tgcgcgcccg cggccacgag ctgctcgaag tacggcagca ggcagattgc gctgcgcctg
      601 aggagtccgg cgttcatatc cgaatgactg cccctgtcga gcgggctcac aaggcctggt
      661 ttcggccaga catccacttc ctgaagcagc gcctcgaccg caagcgtggc gatattgaaa
      721 gtattcattg gcaatcgtgt ggcactgaaa tgccctagcc gacagggtaa ccatacgcca
      781 ctcgcatgat gaacgctccc actgctgtgc gtgggcgata ctcctggcct ccagcagctg
      841 ttctgggcgg agcgtgaaat ggcgcaactg ggcatccgac tcgattagta tcattcgatc
      901 gttgcgaaat gcgcgcaaga gcggtgaacg gcgatggatg cgaagcaata cggtccctgg
      961 gcgctcgtca tcggcggctc ggaaggcgta ggcgcagcgt tcgcacgcaa gctggcggcg
     1021 agcgggttca agctcgtgtt gattgcgcgc aagcgcgggc cgctcgaggc actggcggcg
     1081 cagctgcgcg caagcagcgc tgaagttcgc gtgctctgcg ccgacctggc gcatccggaa
     1141 gctttggaaa gagcgcgcag cgttactgac gagctcgaga taggtctgct catctacaac
     1201 gccggcgcca acaacacgcg cggccggttc gtcgaattgc cgaaagaggt gactcaatcg
     1261 gtcattgcga tcaatgtcat cgggcaggca gattttgccc ggcactacgg ggctctcatg
     1321 tgccagcgcg gtcgcggcgg gatgatcctg gccggatcct taagtggcta catgggatcg
     1381 ccgacactgg ccgcctacac cgcgtccaag gcgttcagcc gaattttcac cgaggcgctt
     1441 tgggcggagt gcaaagcctt tggtgtggac gtgcttcacc tgaacatcgg gttcactgcc
     1501 acccccgcga tggcgcggct cggaatcgat atcagtgtcg ccgaagcgcc cgacaaggtc
     1561 gcgcaggagg gactagacaa catcgcgaac ggtcctttgt ggatcgtcag caccaagggc
     1621 aatatcgaac gtgctcggaa actatcggtc gtcgacgctc gcgccgaact ggtacgctcg
     1681 ttcgctattc caccgcgcga acagatggcg gatctgcaga ggcgccagtt gcaatcctct
     1741 gactagagcg aagaccgccg gatttgccat ctcccgtcta gtgtattatt gtattgatac
     1801 acatggatgc ggatctgctg cacgttcaac cctcggcgcc tgagccgatc tatcgtcaga
     1861 tcgtcgagca gctgcggcgt ctgatcgcca gcggcaagct gctgccaggt gtcgaactgc
     1921 cttcagtgcg cgagatcgcc ggccgtcatg caatcaatcc gatgaccgtg tcacgcgcct
     1981 acagtctgct ggagtatgag ggcctgctgg agcgccaacg aggcaagggc atgatcgttg
     2041 cggccacgag acgtcggcct cagtcagacg acagccgact ggagttgctg cagcctcgcc
     2101 tgcaagaggt ggcgcgtcaa gcgcgtgagc tgggattgcc agatcggcgg gtgctggaac
     2161 gactgcaagc cctattggag gaacacgatg agcgcattgc tcatgccgtc tacaaacctc
     2221 gttgagcccg cccctctcgc ctgcgcggga gtcagcaagc gttttgacgg ctcgacgatc
     2281 ctcagtcgac tggatctgca gattccggcc ggtgcggtgt tggggctgat aggccggaac
     2341 ggcgctggca agaccacgct gatccgcatt ctcatgggtc tgttggcgcc ggatgcgggc
     2401 acggcgaccg tgctcggtga gccggctctg ctgatgagcg acgcggccaa gacgtgtctg
     2461 ggatatgtgc cgcagacccc tgatgcgttg aagtggatgc gagtgggcga gatgttggac
     2521 ttcatcggca ggttctaccc tcgctgggat ccggactatg tcgccgattc gctgctgcgc
     2581 tggactattt caccggaaaa accgctggcc aaactatccc ccggtgagcg ccagcgagtg
     2641 gctttgattc gcgctctggc actgcgtccg cggctattgg tcctggatga acccgccgcc
     2701 tcactcgatc cggtggcacg cagggatctg ctgcgcgaga tcgcactgcg cgcgggtgag
     2761 tccggcacca cggtgctgtt ctccacgcac attgtctcgg atctggagcg cgttgcctcg
     2821 cacgtggcct tcctgcacga gggcagcctg gtactcaagg cgccgatgga tgatctgaaa
     2881 gagacccata cgcggctttg gttacccccc gctggcgcgg cgcagctgac cggcaaattg
     2941 ccgggcgaac tgcggcggcg ccagcttccc gatggcgcgc tgagcatcgt gctggcgcgc
     3001 gaggcaggtg cggcgtggcc ggccgcaacg gcgttgcccg gtgcggatat ggaagtattg
     3061 gggctcgagg atctattcgt agagatcact cgaggtttcc aacctgattt cgcggttggg
     3121 cgtcagcggc tcagactatg aaccgcgact gcgtttcaac ctggcgtgct ctattgggtg
     3181 gcctgcgcgg tgggatggat cgccagatgg tcgtgattgc gatcatcttc gccgcgtttt
     3241 gcgctgcggt ggccatactg tttgcgaccg ccggacttcc gcaggggaag tggattgcaa
     3301 cctccagcct ggcgattgga agttttgcgc tttcgcacct gtgcgctcag tgtctgcgac
     3361 ggctggtacg cggtgcgcag ctgtctttgc tcccggggac cgggccctat gtacggcgcg
     3421 ccgcgctcac tcaatggctg atcatcggcc tggcgccggc actgctcatc ctgacgttgg
     3481 gaggttccga gatccacttc tgtgccgttg ctcttgccgc actcttcggg caagtcactg
     3541 gcacatacgc cgcactgccc ggagcgctgc tcgcagcgac gggagcgttt tttgttgtcg
     3601 tcttccggcg ctcgatagtg ggactgacgc agcagtcgct ttgggtggtc agcgtgctgt
     3661 tgacggtgac gctgctgagc tggctggtgt ggatctggcg cgcgtatttg aggtcggtgc
     3721 cagctgagac ggagttaccg gcgggcagtc gatggggttt acccttgtct cacctcctga
     3781 gatcgcccgg tccggctgga cgacagcagc ctctatctcc aaccatggtg atgcgcacat
     3841 tcatggctgc cccctacgcg cccgccacag cggcacagcg cgcgctgcag gtgggcctga
     3901 tactcgtggt gatgtgtgtg cccgccgcgc tggcttttgc gttgggcagg tcgcacacac
     3961 tgcgtgatct ggagcggctg ctgcatgctg cctgggctcc gaccctgtgg gtaagcggcc
     4021 ttgtgatttg gggatggctg ttgaggggcc aggtgaccag tttttcgcgc ggcagtggca
     4081 acctcgatga actggccttg ctgcccatgc tcggcagtgc agccatgcga cggcgtcagc
     4141 tctatcaggc cacgttgagg ccgcccatat tggtgttgtc gatgctcttc ctggtggcac
     4201 tggttccagt gataggggat gccgattctt ttgggattta cacccgcatc gcctatatat
     4261 tttcgctgct gccggcgttg actctcctcg gctggcagct gcccggtcac cctactagcc
     4321 ggcaggcgtg gatggcggca ccggtggtgc ttttgggcca aagcagtacc gtcttcctgt
     4381 tcagccaccc cgttttcaat gccagggagc tggacctcgt tcgactgata tgtctcacgt
     4441 gttgggtgct tctgggtatc gcagcagtcg aggtcatgct gttcaagagc cgccgactcg
     4501 cggagcgacc acaccccttt gtcgagatcg cgtcgtgagt tcgtccgaca ggtcactctc
     4561 gcgatgccgc gacagacttc gatctgaagg tgttgctcta tggggtcagg atcgcaaccg
     4621 gagattggcg aacagatctg cgcctgcaac cagacgaact ccggtcgttc cgggggccag
     4681 agcgaccgca cgagaggtca gggccaacga atcatcacgg cccactgccc gggcaaacaa
     4741 tcccgccgcg gcgagcgaat ccgcatcaga ctgcctgacc agcacgccac gactcctgga
     4801 atagttgatc agtcctccga cgctcgaccc gatgcagcgt gcgcacgagc acctcagcag
     4861 cgccgtccca ttcgcgccag tgcacgcccg cgccatccgg caagaccagc tcgccatcga
     4921 tgcgcatcgg acacttgcgc gcgcagcggg caatcgagcg caccagttct tctgcctgct
     4981 catggcgggt gacgcgccag agcaggtcca ggtcggagcg ctcatgcaga tacgtgagcc
     5041 cggtgagcat ctgccacagc aggctgccaa ataccgccgg agcgcagccg cagcgctcgg
     5101 agctcgcgag caacgaatgc agtgtctctc gccatgccac cggcgcggca tcgaggcagg
     5161 ccgacagcgc caccggcggc agcggtgcgt ggatctcctc gggttgcagt tgcagcgtgg
     5221 cgcccggttt gcctgcttgc ggtggcagcg ggatcgctac gggaatcaga cccgccgcat
     5281 cctcgggcag atagcgccgc acgatcaccg gccagccgcg accggcccac tctggtacat
     5341 agggtcgcga agcgagcgcc gggctgcgcg ccagggcacg ctgccagccc agggtcgtga
     5401 cgcgcaacag agcgtgccgc tgcagggact caatcacgac gcccggcctg ctgcctgacc
     5461 gcctcggcga cgcgcgccga caactttcgg ccaccgtact gctcgcccgc gcgatcgcgt
     5521 gcatcctcgg gaagttcact catccgccgc agcaatgccg agagctcacc aggcagatcg
     5581 gcgtgcgggt cccacacctc ggtgatggca ccggtcagtt gcatgttggt gagtcccggc
     5641 gcgaacaccg gcgtgctggc ccctatgcgc tgcagcactt gcagcgcaag cttggtgacg
     5701 cgtgagatcg aaggcagatc catcaccgcc gggttggccc cgggcaatgc caccagcgcg
     5761 cgggtcgcaa gcgcggtggc cacgaatgcg cccgcggctc cgccaccgta cagcagcgag
     5821 atcgttggaa aaccgcagcg gtccgcgagc atcaggcact tcgccagatg cgccaggtat
     5881 tcattgagac cgagcagttc atcgcggcgg ctcatgcgct ggctgcccga atcgatcaac
     5941 accagaattg gctgacgctg accgctgcgc acggcggcca gaatgcggtg cgccagcgtc
     6001 agcgcaccct cgacacccag cggcatcgca ttgacgacgc caatgagtgt cagcgcctga
     6061 gccggcgcga ggcgtgcctc gcccagcagg acgccatcgg ctcgttcgtc caccgtgaac
     6121 gagtgtccga acagcgcacg cagcagctca tcgagactca tggctgacat ccggcaggcc
     6181 cgagaccagc gcgatgaatt cctcggtcgg catgtccgcg actttcgatg cgtccggtac
     6241 ccgcagacgg gtccacatct gctgcgcatc gcgcgcgtca ccggcgcgtg ccaggcgacg
     6301 cgcgaggcgc gcgtgttccg cctcggtcgc gaccagatca aacggcggcg gctgatcgat
     6361 cagtctcaag ctcgccttgc ggaagctggc ggcgtcgtct tcgacgaaca gatcggcact
     6421 gccgatcaga cgacgatgct tgccaccgaa cgtgcgccac accagggcgc gatcggccga
     6481 gtcgaattcc tcgactcctt tgttggattc gatgacttcc ggtccggtga caccaatccg
     6541 gccctgctcg gagacgatca gccggctgca gcaggcggcg atcagtgcgc caccgccgaa
     6601 gcagccggcg cgtccaccta ccagcccgat gaccgccacg cccgccgcgc gcgcgatgag
     6661 caccgcgcgc atgatttcgg cgaccgccac ttctccggca ttcgcctcct gcagccgcac
     6721 accaccggtg tcgaacagaa tcagtacgct cgccggcgtg gcggctgccg cacgcaatag
     6781 tcccacgagc ttggcgccgt gaacctcacc gatggcgccg cccatgaaac gtccttcctg
     6841 cgcggcgatc agcaccgctc gtccctcgag ccggccgctg ccgacgatca tgccgtcgtc
     6901 gaacgccggc ggcagatcga acgcttgcag gtgcggactg atcgcgcgct gttccggtcc
     6961 cagcaactcg cgaaagctcg cgctatccaa cagcgcttcg atgcgctcgc gcgcactctg
     7021 ttcgtaccag ctgggcgcca gttcaagcat cctcgcgctc catcacccgc gcaccctgcg
     7081 ccagacgcag cgccacggtg tccgggcgtg cgccaccgtc atgaatggag atgagaagac
     7141 caccagggga atagcgctcg acgaagtcgg cgatcaccgc cgaccacacc tcggcatagc
     7201 cggaaatact ggtcaggacc tcgatccgac attccacgcc agtcagcgtg cgctcgaaca
     7261 gcgcttcgag attgccggag gccaccactc ccagcagcgc ctggcgacgc gctcccggca
     7321 gacgccgtgc ggcctgatgc tgaaacttaa ggctttccat ggccggctag tgtctcacca
     7381 gttgcgaaaa cgcgctggcg gacggtagag cccgcccgac cagcgcacca gatccctgac
     7441 cgagcgcgcg gcgagcaggc ggcgatcggc atccagcgga tcgatgccca gatcctctgg
     7501 acggcggatc acgccacgct cgcgcagtcg tgtcaccatg gcgcgatcgc gcgcacgccc
     7561 gaccgcggtg taaccggcca cgccgcgaat cgcctgctcg cgctcgggcg catcctgaca
     7621 cagcagcaga ttggcgatgc cctcctcggt cacgatgtgc gtcacatcat cgccgtagat
     7681 catgatcggg gcgatatcca gtttgagccg atcggcgaac ttcagcgcat ccagcgattc
     7741 gacgaaggtt ggcgccattt tttcaccgaa cgtctccacc agctgcacca ccagcttgcg
//
LOCUS       DATMRM010000328         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3269789, whole genome shotgun sequence.
ACCESSION   DATMRM010000328 DATMRM010000000
VERSION     DATMRM010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3269789"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /locus_tag="VNZ06_09805"
     CDS             <1..1722
                     /locus_tag="VNZ06_09805"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21087.1"
                     /translation="VVIVTAPATAIGLRIRGRLIESSLPPPLRVSAPEELLPTLSLLS
                     GAHTEPAARVAQLTDIAPWLAHNAWVHFLSPRGLEQYSGGGWGTRDVCQGPVELLLAL
                     DQTPPIRDLLLRVMRQQNPDGDWPQWFMFFERERNIRPGDSHGDIVFWPLLALGQYLI
                     ASGDAEVLNERVPFFDARSPSAGENATVWEHAQRALALVRRRVIPGTSLAAYGHGDWN
                     DSLQPADPKLREHMCSAWTVTLHCQTLNTLADALQQIHRDQDALALREWAGKVHQDFQ
                     RLLIVDGVLTGYAIFDGSAAVRYLLHPRDHSTGVLYSSLAMIHAILEDMLTPQQVREH
                     LKIIDTHLSGPDGVRLFNRPMAYQGGTQRLFQRAETATFFGREIGLMYMHAHLRYTQA
                     MAHIGEAERFFKALCQANPIGIRALVEPASLRQSNCYYSSSDAAFADRYHASDDYERV
                     GRGTIALDGGWRVYSSGAGIALSLIVRRFLGLNPHADHLSIDPVMPAALDGLRVQTRL
                     GGQTIELRYRIGAAGCGVRSVELNGKTLTFDRGPNPHRPGAANIARDAFNAALTGDQN
                     SLQITIG"
     gene            complement(1719..2349)
                     /locus_tag="VNZ06_09810"
                     /pseudo
     CDS             complement(1719..2349)
                     /locus_tag="VNZ06_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000070.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          419 a          695 c          760 g          475 t
ORIGIN      
        1 gtcgtgatcg tgacggcgcc ggcgaccgcc atcggtttgc ggatacgggg gaggctgatc
       61 gaatcctcgc tgccgccgcc actgcgagtg tcggcgccgg aggaactgct tccgactctg
      121 agtttgcttt ctggtgctca caccgaaccc gcggcacggg tggcgcagtt gaccgacatc
      181 gcgccctggc ttgctcacaa cgcatgggtt cactttcttt cgccccgagg actcgagcag
      241 tattctggcg ggggctgggg tacacgcgat gtgtgtcagg ggccggtcga gctgctgctg
      301 gcgctcgatc agacgccgcc gattcgcgat ctgctgctgc gcgtgatgcg acagcagaat
      361 ccggacggcg actggcctca gtggttcatg ttcttcgagc gcgaacgcaa cattcggccc
      421 ggggattcgc acggggacat tgtgttctgg cctctgctcg cgctggggca atacctgatc
      481 gcgtcgggcg atgccgaagt gctgaacgag cgcgtgccat tctttgatgc acgcagtccc
      541 tcggccggtg agaacgccac cgtctgggaa catgcgcaac gcgcgctggc tctcgttcga
      601 cgtcgcgtga ttccgggaac ctcgctggcc gcctacggcc acggtgactg gaatgattca
      661 ttgcagccgg ccgatccgaa gctgcgcgag cacatgtgca gcgcctggac ggtgacgctg
      721 cattgtcaaa cgctgaatac cctggcggac gccttgcaac agatccatcg cgatcaggac
      781 gccctggcgt tgagggagtg ggccggcaag gtgcatcagg attttcagcg actgctgatc
      841 gtcgatggcg tgctgaccgg ctatgccata ttcgacggca gcgcggcagt gcgctatctg
      901 ctgcacccgc gcgaccattc caccggtgtg ctgtatagct cactggcgat gattcacgcc
      961 attctcgaag acatgctgac gccgcaacag gtgcgcgagc atctaaagat catcgacacc
     1021 catctgagcg gccccgacgg cgtgcgcctg ttcaatcgcc cgatggctta tcaaggcggc
     1081 acccagcggc tgtttcagcg cgccgagaca gcgacattct tcggccgtga gatcgggctc
     1141 atgtatatgc acgcgcacct gcgctacacg caggcgatgg cacacatcgg cgaagccgag
     1201 cggttcttca aagcgctgtg tcaggccaat ccgattggaa ttcgtgcgct ggtggagccg
     1261 gcgagcttgc gccagtccaa ctgctattac tccagctccg atgccgcatt tgccgatcgt
     1321 taccacgcca gcgatgacta tgagcgcgtc ggtcgcggca cgattgcgct ggatggcgga
     1381 tggcgagtct attcgagcgg tgcggggatt gcgctgagcc tgatcgtgcg gcgttttctc
     1441 ggattgaatc cgcacgcgga tcacctcagt attgatccgg ttatgccggc cgcgctcgac
     1501 ggcctgcgcg tgcagacccg gcttggcggg cagaccatcg agctgcgcta tcgaatcggt
     1561 gcggccggat gcggagtcag gagtgtcgaa ctcaacggca agacgctgac attcgatcgt
     1621 ggacccaacc cgcatcgacc gggggctgcg aacatcgcaa gggacgcttt caatgccgct
     1681 ctgactggcg atcagaactc gctgcagatt acgatcggct agtgtcctgt cccattaatt
     1741 cgtccgcaaa ggcgggcaag tttgtcgagg attgagtctg ctgtggccgt ccaggtgaag
     1801 ggctggcagg tttggttgta gctgtcggcg aaggcagtga tgcggctggt gaggtccttg
     1861 acgctgcgga acgagccgcg gcggatggcg cgatcggtga tgatcgagaa gaaacgttcg
     1921 acctggttga gccagctgga gtaggttggc acgaaatgca tgtgccatcg cggtcgctgc
     1981 gcgagccagg cgcgcacttt tggatgcttg tggcttgcat agttatcggc gatgcagtgc
     2041 acgtccaact gctctggcac cgccttgtcg atggcacgca ggaactgcag gaactcctgg
     2101 tggcgatgcc gcggccggca ctgagccatc acctgaccgt tcagaacgtt cagcgcggcg
     2161 aacaaggtcg tggtgccgtg acgcacgtaa tcgtgcgtca cgccctcaac gtagcctaag
     2221 cccatgggca acatcggttg cgtgcgttcg agcgcttggc cactggctct tctcatcgac
     2281 acacagcact agtgcattat ccggcgggtt caggtacagg cccactacat cacgcaactt
     2341 ctcgatgaa
//
LOCUS       DATMRM010000329         7074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_516607, whole genome shotgun sequence.
ACCESSION   DATMRM010000329 DATMRM010000000
VERSION     DATMRM010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516607"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="VNZ06_09815"
     CDS             <1..1043
                     /locus_tag="VNZ06_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21088.1"
                     /translation="GAEPEHSAAESSTPAYASPQVLAGMRAEVQDDVFSIASISYGLL
                     SEGARPFGDKTSLEAHRARLCPAVIEGMPVEVFAVLVRSLAGDRLHRPAFAGEFYRDL
                     LGTGPDMACAMRRTLRAGDVGRDVMRRLSFAGVAAAALCAAVLLLPAAQNLIAGTSNS
                     VSAERMAPDVAAAIVPSAAASDTAASQPLTPVSFSPDATGNSDTSPFTLQGATRTSFA
                     PGIVTFESTALIAGSAQSMVAIPIKRLQSTRGAAAFEWEIESGSARPNIDYEPVTPQV
                     VSFRDGQSIRSLFIPLVRTAANGETRPPRSFTVSLRSVDGGARLGSVSRIKVTIVPQP
                     IYSGIGDKLARK"
     gene            1166..2296
                     /locus_tag="VNZ06_09820"
     CDS             1166..2296
                     /locus_tag="VNZ06_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWW21089.1"
                     /translation="MSLFSRLSHELIDIVQWTEDRQDILSHRFQRFNNEIKNGAKLTV
                     REGQLAVMVNEGQLGKDQVADVFKPGMYELSTHNLPILSTLQGWKYGFDSPFRAEVYF
                     FNTRKFTDLKWGTAGPAIMRDSEFGAVRVTAFGIYSIRVKDPKTFLVDLVGTQEDFTT
                     EDIEANLRGKVGIRVKEVMPEIGVPVIDLSSKIVVVGDKIRERIGADFEKLGLELCEV
                     QVQNIGLPEEVEHAIDQQGAMRAIGNMQNFGQYQAAQAIRDAAKNEGSAGAVLGLGVG
                     NMFGQSMGGVLQQPGQVGPGIGAVPATPPPIPGTLAFFIAVNGQQTGPFDLAALRGQI
                     SGGGLTRDTLVWKQGMSAWSKASEQADLAALFNNMPPPVPPQ"
     gene            2301..3398
                     /locus_tag="VNZ06_09825"
     CDS             2301..3398
                     /locus_tag="VNZ06_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21090.1"
                     /translation="MSSVPSRYHPCSGCGAKLEFAPGTLSLKCPYCGAQNAIAAASTD
                     QQAHALERLDFLAYLKDKAGNEPQLERQTVKCPGCGAAVQLPPNVTAERCPFCAGPLV
                     ASDAYAHRAIQPRAVAPFVIKETQARQSFKQWLSGLWFAPNALKQAAHAETGIKGVYL
                     PYWTYDANSQTPYTGERGELYYEDERYLEDGREKVRRVERVRWFPASGQVSVRFNDVL
                     VSASNSVPKAFVQGLYPWKLELLAAYNEDFVTGFTVEAYQLGLEPGFGQAQQQMEVGI
                     REAICRDIGGDRQRIIGMQPHYSDIAFKHILLPIWLSSYRYADKTYRFIVNGQTGTVQ
                     GERPWSAWKIAGAVLLAIVIVVVVVAIRHQH"
     gene            complement(3395..3835)
                     /locus_tag="VNZ06_09830"
     CDS             complement(3395..3835)
                     /locus_tag="VNZ06_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21091.1"
                     /translation="MDKPAPTMKVVSFYHRETGIFNGRHLMVSDPAMIPLNTPPDHIA
                     IDGDHYDHLTQRVDVATGKVIDHIPPQPSEDHEWNSISRCWQIKAEITAKHQARKAAL
                     ARITELELKALRALREHALGRNGAHERLSAIDAEISALREVLRD"
     gene            complement(3865..4791)
                     /locus_tag="VNZ06_09835"
     CDS             complement(3865..4791)
                     /locus_tag="VNZ06_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWW21092.1"
                     /translation="MEQREIGRSGLKVSAIGLGCNNFGWMIGADASYPVIARALDVGI
                     TFFDTADIYGESEVVLGKALGSRRKDLVIATKFGMAASGLPGGASRSYVLQAAERSLK
                     RLGTDYIDLFYLHRPDEATPLGETLEALDELIRQGKVRHAAASNMTAALLSESHAVAS
                     SRQLRGFIATQEHLSLLTRGAEASLIPTVKELHLGLIPYFPLASGLLTGKYQRGVAPP
                     QGTRFDAWKQRSSSLLTEQNFDKVERLEAFAGEHQRSIGELAIAWLLAKPFVPSVTAG
                     ATKAAQVDMNVKAATWKLTAREVAAVERLAPV"
     gene            complement(4854..5357)
                     /locus_tag="VNZ06_09840"
     CDS             complement(4854..5357)
                     /locus_tag="VNZ06_09840"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWW21093.1"
                     /translation="MPLLNPPILISSAGNEAHYALARVLVNEYVAALGVDLSFQGYEG
                     ELQSLERMYAAPRGVLLLAWRDSEAIGCIGVRPLSAKDCEMKRLYVRPSARGLGLGRT
                     LAERAIAWASDSGYRRICLDTLASMAAARALYRELGFEVIEPYNDNPLAGTTFMALAL
                     NRYGSAC"
     gene            complement(5345..5791)
                     /locus_tag="VNZ06_09845"
     CDS             complement(5345..5791)
                     /locus_tag="VNZ06_09845"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWW21094.1"
                     /translation="MKKISIEAIEPRYGSGYPKPFDEPCRQRSRRALGDAAGLTDFGV
                     NVLELSPGTWSSQRHWHSKEDEFVWVLEGKVVLVSEHGEEILDAGDCVGFKAGDPNGH
                     HFQNRSDGVVRLLEVGSRHLDDVSEYPDIDLRWGGPTPTHKDGCPY"
     gene            complement(5801..6436)
                     /locus_tag="VNZ06_09850"
     CDS             complement(5801..6436)
                     /locus_tag="VNZ06_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HWW21095.1"
                     /translation="MFGIHDYWLFIGTGILLNLTPGLDTMFIIGRSLTGGRRAGVASA
                     LGIAVGSICHTLAAALGFSAIIATSALAFTVVKLAGATYLIYLGVMMVCSKNPAAMPA
                     EQALPGPSAQPWPCFRQGIITNVLNPKVALFFLAFLPQFIDPHSPTKALAFLTLGATF
                     ITTGLIWCLILASGAARLRSFFQRNPNVRAIIDRAVGALFVALGVRLVWSR"
     gene            complement(6458..6838)
                     /locus_tag="VNZ06_09855"
     CDS             complement(6458..6838)
                     /locus_tag="VNZ06_09855"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWW21096.1"
                     /translation="MRYEISAQPITTRVCWCQLCQKIGAGSATVNICFSSAAVRIEGE
                     MRDYVSVADSGSVMHRRFCPTCGVHLFSGAESRPHLVFVRAGTFDDRSVVRPAATIWV
                     SEAPAWACIDPTLPKFDRQPPPAA"
BASE COUNT         1354 a         2172 c         2206 g         1342 t
ORIGIN      
        1 tgggcgctga gccggaacac agcgctgccg agagctccac ccctgcttat gcgagcccgc
       61 aggtgctggc tggcatgcgg gcggaagtac aggacgatgt gttcagcatc gcttcgatca
      121 gctatggcct gctgagcgaa ggtgcgcgcc catttggtga taaaacatcg ctcgaggcac
      181 atcgcgcgcg gctgtgcccc gcagtcatcg aaggcatgcc ggtcgaagtg tttgctgtgc
      241 tggtgcgcag tctggccggt gaccgcctgc atcgaccggc ctttgccggc gagttctatc
      301 gtgatctgct cgggaccggg ccggacatgg cctgcgcgat gcgccgcaca ctccgcgccg
      361 gagacgtcgg ccgggacgtg atgcggcgac tgagtttcgc aggcgtcgcg gccgcggcgc
      421 tgtgcgcagc ggtgctgttg ctgccggccg cgcagaacct gatcgccggc acatcgaaca
      481 gtgtttccgc cgagcgcatg gctcctgacg tggcggcggc aatcgttcca tcggctgcgg
      541 cgtccgacac cgcggcctct cagccgctca cgcccgttag cttctcgccg gacgcaaccg
      601 gcaactccga taccagcccg ttcacactgc aaggcgcgac gagaacgagt ttcgcgccag
      661 ggatcgtgac ctttgagtcg accgctctga tcgcaggctc agcgcaatcg atggtggcca
      721 ttccaatcaa gcgtcttcag tccacgcgag gtgccgccgc attcgaatgg gagatcgaga
      781 gtggtagcgc tcggcccaac attgactacg aacccgtcac gccgcaggtc gtgagcttca
      841 gagacggaca atcgattcgc agtctgttca taccgctggt acgtaccgct gccaatgggg
      901 aaacccgacc gccacgctcc ttcaccgtat cgctgcgatc ggtggacggc ggcgcccggc
      961 tcgggtctgt cagtcggatc aaggtgacga ttgtccccca gccgatctac tcggggatcg
     1021 gcgacaagct cgcgcggaaa taaccttaga cgataccctg cgcggccgcc gccgacctcg
     1081 gcctcatttc ggcccattcc cgctcaaagt gacagaacct gagccgccgc ctagaatcgg
     1141 caggccaaca ggacaggagg gcgcgatgag tttgttcagc aggctcagcc atgagctgat
     1201 cgacatcgtt caatggactg aagatcgcca ggacattctt tcacatcgct ttcagcgctt
     1261 taacaacgag atcaagaatg gcgcgaaact gacggtacga gaaggtcagc tcgccgtcat
     1321 ggtcaacgag gggcagctcg gcaaggacca ggtggccgat gtgttcaagc ccggcatgta
     1381 cgagctgagc acgcataacc tgccgattct gtctacgcta cagggctgga agtacggctt
     1441 cgactcaccg tttcgcgctg aagtctactt cttcaatacg cgcaagttta ccgatctgaa
     1501 atggggaacg gccggtcccg cgatcatgcg cgatagcgag ttcggggcgg tgcgtgtgac
     1561 ggcatttggt atttactcga tccgcgtcaa agatccgaag actttcttgg tcgatctggt
     1621 tggtacgcag gaagacttta cgaccgagga catcgaggca aacctgcgcg gtaaggtcgg
     1681 tatccgcgtc aaagaggtca tgccggaaat cggtgtgccg gtcatcgacc tgtcgagcaa
     1741 gatcgtcgtg gttggcgaca agatccgcga gcgcatcgga gcggatttcg agaagctcgg
     1801 gctcgagttg tgcgaggttc aggtccagaa catcgggctg ccggaagaag tcgagcatgc
     1861 catcgaccag cagggtgcga tgcgcgccat cggcaacatg cagaatttcg gtcagtatca
     1921 ggcggctcag gcgatccgcg acgcggcgaa gaacgaaggc agcgccggcg cggtgctcgg
     1981 cttgggcgtg ggcaacatgt ttggccagag catgggcggg gtgttgcaac agcccggtca
     2041 ggtcggcccc ggcatcggtg ctgtgcctgc gactccgccg ccgattccgg gcacgctcgc
     2101 tttcttcatt gctgtcaacg gccaacagac cggacccttc gatctcgctg cgcttcgcgg
     2161 tcagatcagc gggggagggt tgacccgcga cactctggtc tggaagcagg gcatgtcggc
     2221 ctggtccaaa gcctcggaac aggcggacct ggcggcactc ttcaacaaca tgcccccacc
     2281 ggtgccgccg cagtaatcgc ttgagcagcg ttcctagccg ctatcacccg tgctcgggtt
     2341 gcggcgcgaa gctggagttt gctcccggca cactgtcgct gaagtgcccg tattgcggcg
     2401 cgcagaacgc cattgcggcc gccagcaccg atcaacaggc gcacgccctg gagcggctgg
     2461 acttccttgc ctacctcaag gacaaggcag gtaacgagcc gcagctcgaa cgtcaaaccg
     2521 tcaagtgccc tggctgcggc gctgctgttc agttgccgcc gaacgtgact gcggagcgct
     2581 gcccgttttg tgccgggccg ctggtggcgt ctgacgcgta cgcgcaccgc gcgatccagc
     2641 cgcgtgcggt ggcgcctttc gtcatcaagg aaacgcaggc cagacagtcg ttcaagcaat
     2701 ggctatcggg gctgtggttc gcgcccaatg cgctcaagca ggcggcgcat gcggaaacgg
     2761 gcatcaaggg cgtctatctg ccgtactgga cctacgacgc caacagtcaa acaccgtaca
     2821 ccggtgagcg cggcgagctc tactacgagg acgagcgcta cctcgaagac gggcgcgaga
     2881 aggttcgacg ggtggagcgc gtgcggtggt tcccggcttc aggccaggtg agcgtacggt
     2941 tcaatgatgt tctggtgtcg gcgtcgaatt ccgtgcccaa ggcgttcgtg caaggcctat
     3001 acccatggaa actggagcta ctggccgcgt acaacgaaga cttcgtgacc ggctttacgg
     3061 tcgaagccta tcagctcgga ctcgagccgg gttttggcca ggcgcaacag cagatggaag
     3121 tcgggatacg cgaggccatc tgccgcgata tcggtggcga tcgccagcgc atcatcggca
     3181 tgcagccgca ttattcagac attgcgttca agcacatcct gctgccgatc tggctgtcat
     3241 cgtatcgtta cgcggacaag acctatcgct tcatcgtcaa tggccagacc ggtaccgtcc
     3301 agggtgaacg gccttggtcg gcctggaaaa tcgcgggcgc ggtgttgctg gcgatcgtga
     3361 tcgtcgtggt cgtcgtcgcg attcggcacc agcactagtc gcgcagcacc tcacgcagcg
     3421 ccgaaatttc cgcgtcgatg gcgctcaggc gctcatgagc cccgttcctg ccgagcgcat
     3481 gctcgcgcag tgcacgcagt gccttgagct cgagctccgt gatgcgtgcc agcgctgctt
     3541 tgcgtgcttg atgcttcgca gtgatctccg ccttgatctg ccagcatcgc gaaatcgagt
     3601 tccattcgtg gtcttccgag ggctgcggcg ggatgtgatc gatcaccttt ccggtggcga
     3661 catccacgcg ctgtgtcaga tgatcgtagt gatccccgtc aatcgcaatg tgatcgggcg
     3721 gcgtattgag cggaatcatc gctggatcgg acaccatcag gtggcggccg ttgaatattc
     3781 cagtttcgcg atgatagaaa gacacgacct tcatcgtcgg cgccggcttg tccatcgctt
     3841 gtctccccaa gagttccgtg atgatcacac cggggccagt cgctcgacgg ctgcaacctc
     3901 ccgtgctgtc agcttccatg tcgcggcctt cacgttcata tcgacctgcg cggccttggt
     3961 cgctccggcg gtaacgcttg gaacgaaagg cttggccagc agccatgcga tcgccaattc
     4021 gccgatcgac cgttggtgtt ccccggcgaa agcctccagc cgctcgactt tgtcaaagtt
     4081 ttgctcggtc aacagcgacg aacttcgctg tttccacgca tcgaaccgtg ttccttgcgg
     4141 cggggcgacc ccgcgttggt atttgccggt cagaagccca ctcgccagcg ggaaataggg
     4201 gatcaacccc agatgtaact ccttgacagt ggggatcagg ctggcctcgg cgccgcgcgt
     4261 cagcaggcta aggtgctcct gagtggcgat gaagccgcgc agctgcctgg acgaagcaac
     4321 ggcgtgcgat tcgctcaaca gcgcggccgt catgttggag gcggcggcat ggcgcacttt
     4381 cccctggcgg atgagctcat cgagcgcctc cagcgtctcg ccgagggggg tcgcctcatc
     4441 gggacggtgc agatagaaca ggtcgatata gtcggtgccc agtcgcttga gactacgctc
     4501 cgcggcctgt aggacatagc tgcgcgaggc gccgccgggt agtcctgacg ccgccatgcc
     4561 aaacttcgtg gcgatcacca agtctttgcg ccggcttccg agtgccttgc ccaatacgac
     4621 ctctgactcg ccgtaaatgt cagcggtgtc gaagaaagtg atcccgacgt ccagcgctcg
     4681 ggcgatgacc ggataggacg cgtcggcgcc gatcatccag ccaaagttgt tacaacccag
     4741 gccgattgcc gataccttca gacccgatcg tccgatttcg cgctgttcca tgagaggata
     4801 gtcccttaag gggaagtggc tcgattctag tcgagcagcg ctcgtcccgc ctgtcagcaa
     4861 gcgctaccgt agcgattgag agccagcgcc atgaatgtcg tgcccgccag aggattgtcg
     4921 ttgtacggtt cgatgacctc gaagcccagc tcgcgataga gagctcgtgc ggccgccatg
     4981 gatgccaggg tgtcgaggca tatgcgtcga tagccgctgt cggacgccca ggcgatcgcg
     5041 cgttcggcca gtgtgcgccc gaggccaagc ccgcgcgcgg acggacgcac gtacagacgt
     5101 ttcatctcgc aatctttggc actgagcgga cgcacgccaa tgcagccgat cgcctcgctg
     5161 tcgcgccacg cgagcagcag cactccgcgg ggcgctgcat acatgcgctc gagactttgc
     5221 agttcgcctt cgtatccctg aaagctcaag tccacaccga gtgctgcgac gtattcattg
     5281 accaacacac gcgccagcgc gtagtgcgcc tcgttccctg cagaggaaat gaggatcggc
     5341 gggttcagta ggggcaccca tccttgtgcg tcggggtcgg gccaccccag cgcaggtcaa
     5401 tgtccgggta ttcactgaca tcgtccagat gccttgatcc tacctcgagc agtctcacga
     5461 ctccgtcgga tcgattctga aaatgatgcc cgttgggatc gcctgctttg aatccgacac
     5521 agtcgccggc gtcgagaatc tcctcgccgt gttcgctcac cagcaccacc ttcccttcga
     5581 gcacccagac gaactcgtct tccttcgaat gccagtggcg ttgcgaagac caggtaccgg
     5641 gtgacagttc cagcacattg acgccgaagt ccgtgagacc ggccgcatcg cccagcgcac
     5701 ggcggctgcg ttggcggcag ggctcatcga agggcttcgg atagccggag ccgtagcgtg
     5761 gctcgatggc ctcgatggat atctttttca tgaaatcctc ttagcgcgac caaaccagac
     5821 gtacaccgag cgctacgaat aaagcgccga cggcacgatc aatgatagct ctgacattgg
     5881 ggttgcgctg aaagaacgaa cgcagtcgcg cagccccact ggcgaggatc agacaccaga
     5941 tgagtccggt tgtaatgaac gtcgcgccca gtgtcagaaa tgccagcgct ttggtcgggc
     6001 tgtgagggtc gatgaactgc ggcaggaaag ccagaaagaa cagcgcaact ttgggattca
     6061 gtacgttggt gatgatgcct tgccgaaagc acggccatgg ctgcgcggaa ggaccaggga
     6121 gcgcctgctc cgcgggcatg gccgccgggt tcttgctaca gaccatcatg acaccgaggt
     6181 agatcagata tgtggcaccc gctagcttca cgacggtgaa ggcgagcgcc gaggtggcga
     6241 tgatcgccga gaagcccaat gctgcagcca gggtgtgaca gatgctgccg acggcgatac
     6301 ccagcgccga ggccacgcct gcccggcgtc cgccggtgag gctgcgaccg atgatgaaca
     6361 tcgtgtccag gcccggtgtc agattgagca gaatcccagt gccgatgaac agccaatagt
     6421 catgaatgcc gaacatggaa ccttccgtct gtggcactca ggcggcgggc ggtggctgcc
     6481 gatcgaactt cggcaaagtc ggatcgatgc atgcccaggc tggggcctcc gacacccaga
     6541 tagtggcggc cggtcttacc accgaacgat cgtcgaaggt gcccgcgcgc acgaacacca
     6601 gatgtgggcg gctttccgcg ccgctaaaca ggtgtacacc gcaggttggg cagaagcgtc
     6661 ggtgcatgac gctgccgcta tcggccacgc tgacgtagtc gcgcatctca ccttcgatgc
     6721 ggaccgcggc gctcgagaaa cagatattga ctgttgcgct accggcgcca attttctgac
     6781 acagctgaca ccagcagaca cgggtcgtaa tgggctgcgc gctgatttca tagcgcacac
     6841 ccccacaaag gcatcctccg ctgaacggca cagctccctc cctcccgatg tattcgtacc
     6901 ttagggttgc aggtacctac caaaccaagc taacacacgc cgttgtgcat cttccagatg
     6961 tgccggatcg cgcagcgcgt gaccttcgtg cgggtagatc accatcgcgg tgggcacatg
     7021 cagatcccgc agtgcgtgcc agaactcctc ggtctgcggc gccgggcatt cgat
//
LOCUS       DATMRM010000330         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1766177, whole genome shotgun sequence.
ACCESSION   DATMRM010000330 DATMRM010000000
VERSION     DATMRM010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766177"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VNZ06_09860"
     CDS             <1..428
                     /locus_tag="VNZ06_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21097.1"
                     /translation="IQDLKKDVVDLNKDLFVLEEELLFPANTQVAVFVSVDVGDFFAL
                     DSITLKIDNRNVTNYLYTPRETAALLKGGVQRLYVGNLKAGEHELVAFFNGKGPHDRF
                     YRRGANLRFEKGVGAKYLELKVTDHAHAQEPEFEIKDWE"
     gene            436..>2176
                     /locus_tag="VNZ06_09865"
     CDS             436..>2176
                     /locus_tag="VNZ06_09865"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWW21098.1"
                     /translation="MRVRRLCLGLVCVSLLAAVAPAAWAKHKPDPHKLPSVRIQDLHY
                     GDVLFHYWSDEDSGLQTLTLLGAYSQWNLLQHHETDAQLLAAGLYLQLGMHNEAGNRF
                     ETLLGDRLPPAVRNQAWFYLGKIWYERGYYDRSEDALKHIHGQLAPQAEAERTHLLVN
                     VLMRQGRFDEAIAVLKNWHGPADWMAYAQFNLGVALVRANRLPEADPILTAVGTVHTY
                     SPELLNLRDKGNLALGYAYLQADMPAQALVPLARVRLEGPYSSRALLGDGWARAALKD
                     YQAALAPWLELHKRGLLDAAVQESYLAVPYAYGQLGAATQAAEAYEDALKSFASESDN
                     LDHAIENIRAGHLLDDLLSSDKSNANTYGWFYQLRSLPDSPQSRYLYSLLADNDFQEG
                     LKNWRDLGYLQRSLARWDDSMDAFAAMLDTREKAYANLLPKADALIAGNAPAHLSDAR
                     AAADSRLNAIEIGDDVAALGTPQEREQWAQLLKLEEAANAMPSSPERDAAVDKIRIIK
                     GVLYWRLDAAFKQRSYDQRHALHELDAQLDELQNRWVRVQKARATVPTNTGDFADRIA
                     GLAERIKSVRQRLA"
BASE COUNT          429 a          650 c          709 g          388 t
ORIGIN      
        1 agatacagga tctgaagaag gacgtcgtcg atctcaacaa ggacctgttc gtgctggaag
       61 aggaactgct gttccctgcg aacacgcagg tcgcggtatt cgtgtcggtg gatgtgggtg
      121 atttttttgc gctcgattca atcacgctca agatcgacaa ccgcaacgtc acgaactacc
      181 tgtatacacc gcgcgagacg gcggccctgc tcaagggcgg tgtacagcgg ctgtacgtgg
      241 gcaacctcaa ggccggcgaa cacgaactgg tggcgttttt caacggtaag ggcccgcacg
      301 accggttcta tcgccgcggc gccaacctga ggttcgagaa gggtgtgggt gcgaagtacc
      361 tggaactcaa agtcaccgat cacgcacacg ctcaagagcc tgagttcgag atcaaggact
      421 gggaataatc cgggcatgag agttcggcgc ctttgcctag gcctggtatg cgtatcgctg
      481 ctggcggctg tcgcgccggc ggcgtgggcc aagcacaagc ccgatccgca caaactgcca
      541 agcgtcagga tccaggacct gcactacggt gacgttctgt tccactactg gtcggatgaa
      601 gactcgggtc tgcagacgct gacgctgttg ggcgcgtatt cgcaatggaa tctgttgcag
      661 catcacgaga cggacgcgca gctgttggct gctgggcttt atctgcagtt gggtatgcac
      721 aacgaagccg gaaatcgctt cgaaacgctg ctcggcgacc gtctgccgcc ggccgtgcgc
      781 aatcaggcgt ggttctatct gggaaagatc tggtacgagc gcggctacta cgatcgcagc
      841 gaggacgcgc tcaagcatat tcacggccag cttgcgccgc aggcggaagc tgagcgcacg
      901 catctgctgg tcaatgtgtt gatgcgccag gggcgcttcg acgaggcgat cgcggtgctg
      961 aagaattggc acggtccggc ggactggatg gcgtacgcgc agttcaacct cggtgtggcg
     1021 ctggtgcgcg caaaccgcct gcccgaagcc gatccgatcc tcaccgcggt cggcactgtg
     1081 catacctata gcccggaact gctgaacctg cgcgacaagg ggaacctcgc cctcggctac
     1141 gcgtacctgc aggcagacat gccggcacag gcactggtgc cgttggcacg ggtgcgcctg
     1201 gaaggaccct attcgagccg cgcgctgctc ggcgatggct gggcgcgcgc cgcactgaag
     1261 gactaccagg cggcactggc gccctggctg gagctgcaca agcgcgggct gctcgacgcc
     1321 gcggtgcagg aatcctatct ggcagtgccg tatgcctacg gccagctcgg agccgccacg
     1381 caggcagcgg aagcgtacga ggacgcgctg aaatccttcg ccagcgagtc agacaatctg
     1441 gatcatgcca tcgagaacat tcgcgccgga catctgctcg atgatctgct gtcctccgac
     1501 aagagcaatg ccaatacgta tggctggttt tatcagctgc gcagtttgcc ggactcgccg
     1561 cagtcgcgct atctgtactc gctgctggcc gacaacgatt tccaggaggg tctgaagaac
     1621 tggcgcgatc tgggctacct gcagcgctcg ctcgcgcgct gggatgacag catggatgct
     1681 tttgccgcga tgctcgacac gcgtgagaaa gcctacgcca acctgctgcc gaaggccgat
     1741 gcgctcattg ccggcaacgc gccggcgcat ctgtcggatg cgcgggcagc ggccgactcg
     1801 cgactgaatg ccatcgagat cggcgatgac gtcgctgccc tgggcacgcc gcaggaacgc
     1861 gagcaatggg cgcagcttct gaagctcgaa gaagcagcca atgcaatgcc gtcaagtccg
     1921 gagcgggacg cagcggtgga caagatccgc atcatcaagg gcgtgctcta ctggcgcctg
     1981 gatgccgcgt tcaaacagcg cagctacgat cagcgccatg cactgcatga gctggacgcg
     2041 cagctcgatg aactgcagaa ccgctgggtg cgggtgcaga aggcgcgtgc cacggttccc
     2101 accaataccg gcgactttgc cgatcgcatc gctgggctgg ccgagcggat caagtccgtg
     2161 cggcagcggc tcgcgg
//
LOCUS       DATMRM010000331        13844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_173627, whole genome shotgun sequence.
ACCESSION   DATMRM010000331 DATMRM010000000
VERSION     DATMRM010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13844
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173627"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VNZ06_09870"
     CDS             complement(<1..655)
                     /locus_tag="VNZ06_09870"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HWW21099.1"
                     /translation="MSRDRVRFLLTLALGTAAIGIGTLARADNAPPTIPTCDKKIGTL
                     SVKEPPNNWWAEYHLDSPESLIKVMVSQSKCFTLVDRGKGLEAAQSERALASSGDLRG
                     GSNVGKGQMKAADYVLVPDIANRNRNSGGTNIGGAIAGLIPGGFGAALGGVSLKSKTA
                     DVVLTLTDVRSTEQVALEQGHAKKTDLGWAGGGGGFLGAFVAGGASSYANTEIGQVVM
                     "
     gene            complement(750..1280)
                     /locus_tag="VNZ06_09875"
     CDS             complement(750..1280)
                     /locus_tag="VNZ06_09875"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWW21100.1"
                     /translation="MALIPAGLGLGSYAIMRNEASVADYNLYCGNTKGCTPVSGSDPD
                     LPMVNISYDDIQKYAAWLSQATGARYRVPTRPEWVRAAGGRSKTDSDANCVVPGRDPR
                     GTSLRAATAGNANVYGLHNMFGNAQELTTTVNGGLAAVGGAIGDDLIYCQSEFLRPAN
                     GQPEGRTGFRLLREMQ"
     gene            complement(1817..4795)
                     /locus_tag="VNZ06_09880"
     CDS             complement(1817..4795)
                     /locus_tag="VNZ06_09880"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWW21101.1"
                     /translation="MSEFRAALEQFLAGTLGADEARRRVSAAAASEPQVAQAMLAVIE
                     AYRSSRRLTPEFAAELQQLVRQSAGLPTAATAPDEPTHLSVRGPPPPAAVSPPPPPPP
                     PPPPQAAAPPSPPSPRPASSPRSSSAGGWGTQVTVGTVLKDRFVLETLVAGGDKGGMG
                     VVFKALDRIKQEAQDRNPYVAIKVLNEDFKQHPDSMMALQREARRAQTLAHPNIITVY
                     DFDRDGDTVYMAMELLIGSSLDEVIRTHRDRGGLPVKDALQIITQLGRGLSYAHANNI
                     VHSDFKPSNAFMTKDGVVKVLDFGIARATKLPDGEKTRFDAGSLGAMTLPYASCEQLE
                     RQDPDPSDDVYALSIVSYELLTGRHPFERPDPDKPGKLLRTDSITARNGKMKPAPAPI
                     PGLSRAQGRTLQRGLSFRRAGRPRNAAEFLEGMTPRKVPVGALIAAIAAGVLLLVTTT
                     LLVSSYVQKSRLQALTQKLESNDPAVIAAALQSLQKYPPDERAPVLLNDAVQGNLINY
                     YIQRAHEQFNVEAARYDYAGALATLKEAQGLSKVYEDSRQLNDALDKLESDRKAEILK
                     QAQAIDSELSQVMLIASQGAQNVRSTLAIIRQLDPNHPLLNDKRVPIAFANQVRSNMD
                     AEHLAVAAALLAAGLQFAPNDPGLLDLQDRVTRQQNSAQLSVQAGELEQAVGPLAQPG
                     STLADFRSKRAQLQSLQQAQPNSPVLASAQDRLSSLIDPIVSDAVARSQVTDAQATLN
                     EFADLLPAAVLAKERSQIASVTGNTQPREDTTAQLRAKIEREAANPKSDEASVSGLQR
                     DLQDLQGLAGASDPGIAEVRSRVSQAYLADSRRMLGKAHFTEAQHLLDLARQFGLPAD
                     AYQTQSSALTQARAQAEAENRANENAARLSAAKQRVLDEATADHIDVAQTQFAELKKS
                     LPATDPFVTTDAPRAIPRPIWNARAMLRSKCTSMRRCRMPRPRSRRRPICRRSPMSCS
                     CFAVRATWLICWRALRIFAR"
     gene            5050..7800
                     /locus_tag="VNZ06_09885"
     CDS             5050..7800
                     /locus_tag="VNZ06_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWW21102.1"
                     /translation="MTVCAALFATAVQSSDHSAETATRAQIAAASFEDTIVVDCQLPG
                     RVQQLGAARTYLTAGPLMRLPAIDCRTRGGEYTMGDLASGTLSLKRWLPLANQDNVEA
                     QYYVARIYANGMDGVPVDYELAAKWYQRAVQKKYAPAMEELGYLYEQGLGVPKDPVAG
                     INLQRAASGLGEDLVYASTLAANKEQAEAQIALLTDDLKSADAVVVDLQGQLTQADDK
                     LAQNHSQLSEDQARLRDLRSKLEQARRDGGTADLANVQRLQQQLAASEAALKDKQDAI
                     NVLSADQGLRQAQLSAQLARSQATNTQLSNMLAAGQSENESLRARLAQAEQRAVQSQQ
                     EVASVRADFVRETRELSARSAELTQLREHGSDGLQSLLSSKQGELERQQKLLASLESQ
                     LASLRQQVSSAGSGSSEASARNKELEATLAALRIQYAQQQQQLQDQRADFARLQSQSK
                     DDRSALLARLSAQLDQKSAELQDKQHRLDSLQAESSRLRDDYNREHDARARDGTLSAS
                     QLQQDKEALRVAQDQLAQQRASLQQLELQSATQQLQLVQERERFARALAGGQQADQQR
                     IATLEAGVRARDQQIADARARVSTLEQQNSVAKTVSTAPANNAVSLSTPVSKSPVDTG
                     TTSTASAGTTSTVSYRLPQSAAASGNPFDMVQLVKGLGPANYYALVIGNSKYEKMQAL
                     KTAANDARDVAKLLEGRYGFQVKLLIDATRADIGRALYEYARSLSDSDRLLIYYAGHG
                     GSRVFPPERAYWTGVDSDPDNASSWIGAQDVSDDIAQIHARHILLVADSCFSSVITHQ
                     TSTIVAPSNDERSVRIKWARSARMVLTSGRNEPVVDSADPQQTHSLFAEQFLTVLRQN
                     TILLSGQGLAHELSGRVEDAAARTGLKQTPTYSNLQDQQHMFGDFFFVPLTSVQVAST
                     AR"
     gene            complement(7787..8632)
                     /locus_tag="VNZ06_09890"
     CDS             complement(7787..8632)
                     /locus_tag="VNZ06_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21103.1"
                     /translation="MRARRRPIRWSSLNVTLWCLLLGACASATQRARDFASQHALTDS
                     LIQGEAFEHQVFWSSHPDGKVLYVFIDGDGVPWSGHGTRVASDPTPLHPLALALAART
                     AHPVLYLGRPCYFSVHDRAGCTPGVWTSGRYSAQVVDSMAVAANGFAAAGDYQHLVLI
                     GYSGGGALAVLMAPKVPSTRAIITIAANLDVGEWARVHGYLPLDQSLDPATQPALPSA
                     IAQRHLFGGRDLTVPERSTVRYFQRLQASQLWRFPGFDHVCCWIRHWPDILKHLDEEM
                     ALNAP"
     gene            complement(8592..11291)
                     /locus_tag="VNZ06_09895"
     CDS             complement(8592..11291)
                     /locus_tag="VNZ06_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21104.1"
                     /translation="MKRQFQRLVRVTVYLATALVAACGGSSSSEFVQTPTALIATIGN
                     QPSRGSSAGSVSITVRAGADVQLSGEDSYGGNVSITGFGWTQTAGPAVTLLYRNSSTV
                     SFTAPSLSQSATLKFQLSVTNASGLTGTATAQVQVVPANDLNRFLNTQAAAPPLLPHT
                     FRVAVSTVAGLAGPTADVPVCVQLTRTVIYSPRQGGAESGAVTLPTKQIDAKWVAGVA
                     AAPGFSTPLSFSNPLVSFPLPVLNSEDVFVAYNAPGANLNNNQLVDSDVDTAFVRMAV
                     SATPGSCDGSQAGSALSASQLMLQLYDEAGDALGTPATASTAGTAVTIDTPLTSLPLT
                     PDGSASLTPEDFLRAQALTAGGPPSSFETRESAGAYYAAIDPNSQRSTLQGWLQQNCF
                     DPNDRNYGEGEAGFSVTHADYANNFDLAFGRDMYFANCTNGNMASVVVNYPSLGAAVN
                     RIGAFLAVAMEYTPAPGSTAPCFTNPADSTTNTGACYAKFYAFAPDDRTGVFQRVLSA
                     NFDRRGQRYLPGACTACHGGAPGFTPGQPYPAAGDVDATFMPWDVGAMLFADTDPAFP
                     CQVSTLSPVCGAVDASKFTQSAQQPRIQQLNALAWRTYTAPEMITTGSGASVDRYAAA
                     RALMSKWYGGDPAASTAHPFDDSATPDAWLTAGQSTGASNDLYHAVFRHYCRACHTQV
                     NGGAQIGNVQFQSLASFLFLDQPLAPGLPAFLQSLVFTQGTMPQSRLTADRFWVDFGG
                     GQSAAQTLATYLNSLPGATPVAVDSAQNVIGPGAPQLVTLNGRAALVSTEASPNASNT
                     LAAVAGILLDAASQSVFIGSYQWSLCSGGPPASPGGACPGSLLGLIGTPIVPAGSSAG
                     AAEAGAAFPAFATPVSGTYYLSVTAQGSVSGASTTATYPLVVP"
     gene            complement(11321..12829)
                     /locus_tag="VNZ06_09900"
     CDS             complement(11321..12829)
                     /locus_tag="VNZ06_09900"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HWW21105.1"
                     /translation="MRRGLSLACGALLLQIVGASAWAQTAPAPNGGSGCTIGVAGDGA
                     VPAPCLTTLVTTDNAFNFNGLELAAAQADDAAYVSLLKLCGLTGSGCSGAQLNLFIQL
                     RELEDNAAELLGFGQSAFSLHLSAQALGFALRWTADEEYSAQSSITNRLASNQFDAVT
                     NRLNAVRFITQTMRFARQGYQDDEQLADADAEQALGGGASADENLPGVGRWSVFASGS
                     YGSGSKAPTTFNDAFDFSGQQFLFGADVRLSAHSVFGIIGSEVSQHANFNSSESIASG
                     NVKGSGGGATVYFEQDWNSAYLNVSVGVQHMTLDTRRLVAYPSNNPQEPSVNTNFFSS
                     TGATSWLGTVGGGYSWYLHAFSATPYANLQYLGTRIGAFTETGSGSDPELALSVNGQS
                     VTSLQSILGLKFNYAWSTRLGVIIPYGYGEYRHEFRDKPQSVSSHYVSSQSVADQFQL
                     PTDNIDPNYWEVGGGVITELPHRFQLYAQYLKVLQLQYYSYYAISGGFRFTF"
     gene            complement(13058..>13844)
                     /locus_tag="VNZ06_09905"
     CDS             complement(13058..>13844)
                     /locus_tag="VNZ06_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21106.1"
                     /translation="AALEQWNRDPPDPRDDIYAFACVAYFIFGGKHPFARASARTAFE
                     AQLVPQRIDSLSRRQWDGVRRGLALRRADRIESVSEFLRLLAPQTWLQKNRLWIGALV
                     ATVLAMALFLGARFYSEYVQDQALNAQLWPPAVSAPEPVTEAQCRQIGDTLFLSADAL
                     KQASSMASTDEVEALLSKGANNLHDMLTQVLQCNPDNAQAKQIISQAARAYAGLARAR
                     LQQNDTAQALRLVTDGQNFEHNLQLLRLKQGICRRDPKICRAE"
BASE COUNT         2483 a         4458 c         4477 g         2426 t
ORIGIN      
        1 gcatcaccac ctggccgatc tcggtgttgg cgtagctgct ggcgccgccg gcgacgaatg
       61 cacccaggaa gcccccgcca ccgccggccc agccaaggtc ggtcttcttg gcatgaccct
      121 gctcgagcgc gacctgttcg gtcgagcgca catcggtcaa tgtcagcacc acatcggcgg
      181 tcttgctctt gagactcacg ccaccgagcg ccgcgccgaa gccacccggg atcaggcccg
      241 cgattgcgcc gccgatgttg gtgccgccgg aattgcgatt acggttcgca atgtcaggca
      301 ccagcacgta gtcagcggct ttcatctgac ccttgcccac attcgagcca ccgcgcagat
      361 cgccgctcga agcaagcgcg cgctcggact gggccgcctc cagacccttg ccgcggtcca
      421 ccagcgtgaa gcacttggac tgcgacacca tgaccttgat cagggactcg ggtgagtcga
      481 ggtgatactc cgcccaccag ttgttgggtg gctctttcac cgacagtgtg ccaattttct
      541 tgtcacaggt cggaatggtt ggcggcgcat tgtcagcacg cgccagcgta ccaataccga
      601 tcgcagcggt gcccagcgcg agcgtcagca ggaaacgtac acggtcacga gacataatcg
      661 gctcccttcc atgaatccgt cggtgggtca cgcccacaca atattgcgtg gccgggacgt
      721 atagttatca cgctatctta gcgcaactcc tactgcatct cacggagtaa gcggaaacct
      781 gtgcgcccct cgggctgccc attggcaggt ctcaggaatt ccgactgaca gtagatcagg
      841 tcgtcgccga tcgcaccgcc caccgcggcc agcccaccat tgacggtcgt ggtcaattcc
      901 tgcgcgttac cgaacatatt gtgtaaaccg tagacattgg cattgcccgc ggtcgctgcc
      961 cgcagcgacg taccacgcgg gtcgcggccg ggcaccacgc aattggcgtc cgaatcggtc
     1021 ttgctgcgac cacctgcggc gcgcacccat tccggtcggg tcggcacacg gtatctcgca
     1081 ccggtcgcct ggctgagcca ggcagcgtat ttctgtatgt cgtcatagga aatgttgacc
     1141 atcggcaagt caggatccga tccactcacc ggtgtgcagc ccttggtgtt gccgcaatac
     1201 aggttgtagt cggccacgct cgcctcgttg cgcatgatgg cgtacgagcc cagccccaga
     1261 cccgcgggaa tcagtgccat ctccggcccg cgcccacccc ttgtcagatt gtcgcggcag
     1321 aagatcaacg tgctggcgcc gggcgcaatc gcactgcagg gaccgaccgg ccccgcgctg
     1381 agcgcgggag caacgctctg cgcattgccc tgtgacagcg tctgcgcggg cggttcctga
     1441 ctcaccggcg gcggcgggcg gttgtcgttg gcctgtcgtt gctggcgctg attgggcggc
     1501 ggcgtgctcg tgacctgtga ttcgggcaca gccggggtct gctgtggcga cggcgcgatc
     1561 tggttctgat tctggttttg attctggttt tgattctggt tttgattctg ggcctgaacc
     1621 ttattctgcg gagccttcct cagctccgcg acgtgaggaa agatcggaag cgcactggcc
     1681 tgaaggcgcg cggcttccgt cgggttctgg gcgttcacgc gattgatgcg ctcggccacc
     1741 aggcgcccca gaccgattgc aattgctttg gaaccctggt cgtgttcgct ctggcgtacc
     1801 tgattgacct gcccgctcaa cgggcgaaaa tccggagcgc tctccagcaa atgagccacg
     1861 tcgcgcgcac cgcgaaacag ctgcagctca tcggcgatct ccggcagatt gggcgccgcc
     1921 tgctgcgcgg cctgggcatc ctgcagcgcc tcatcgaagt gcacttgctt cgcagcattg
     1981 cgcgcgcgtt ccagataggc ctcggaatgg cgcgcggggc atcggtggtg acgaacggat
     2041 cggtggccgg cagcgatttt ttcaactctg cgaactgggt ctgcgccaca tcgatgtgat
     2101 cggcggtcgc ctcatcgagc acgcgctgct tcgccgcaga gagccgcgcc gcgttttcgt
     2161 tcgcacggtt ctcggcctcg gcctgcgccc gcgcctgcgt cagcgcggag gattgtgtct
     2221 gataggcatc cgccggcagg ccgaactgcc tggccagatc cagcaggtgc tgcgcttcag
     2281 tgaagtgggc ttttcccagc atgcgtctgg agtcggcgag ataagcctgg ctcacacggc
     2341 ttcgaacctc ggcgatgcca ggatcggagg cacctgccag cccctgaaga tcctgcagat
     2401 cacgctgcag tccactgacc gaggcttcat cgctcttcgg gttggcggct tcccgctcga
     2461 tcttggcacg cagttgtgcc gtggtgtcct cccgcggctg ggtgttgccc gtgacactgg
     2521 cgatctgcga gcgctctttg gcaagcaccg ccgccggcaa caggtcagca aactcattga
     2581 gcgtggcctg cgcatccgtc acctggcttc gcgccaccgc gtcgctcacg atcggatcga
     2641 tcagcgagct cagccgatct tgcgccgaag ccaataccgg gctgttgggt tgtgcctgct
     2701 gcaacgactg cagttgcgcc cgcttcgagc ggaagtccgc cagcgtcgag cctggctggg
     2761 ccaatgggcc cactgcctgt tccagttcgc ccgcctgcac gctgagctgc gcgctgttct
     2821 gctgacgcgt gacccgatcc tgcagatcca gcagcccggg atcattgggc gcgaactgca
     2881 accctgccgc cagcagcgcc gcggcgaccg ccagatgctc agcatccatg ttgctgcgaa
     2941 cctggttggc aaaggcgatg ggcacacgct tgtcattcag cagcggatga ttcggatcga
     3001 gctgacggat gattgccagc gtcgagcgca cgttctgcgc accctgactg gcgatcagca
     3061 ttacctggct cagttcgctg tcgatcgcct gcgcctgctt caggatctcg gccttgcggt
     3121 cgctctcgag cttgtccagc gcatcgttga gctgccgcga atcctcgtac actttgctca
     3181 ggccctgcgc ttccttcagg gtcgcgagcg ccccggcata gtcatagcgc gcggcctcga
     3241 cgttgaactg ctcatgcgcg cgctggatgt agtaattgat cagattgccc tgcaccgcat
     3301 cgttcagcag caccggtgcg cgctcatcgg gcggatactt ctgcagcgac tgcagtgcgg
     3361 cagcaatcac ggccggatcg ttggattcca gtttctgcgt caacgcctgt aggcgcgatt
     3421 tctgcacgta cgaggagacc agcaacgtgg tcgtgaccag tagcagcacg ccagcggcga
     3481 tcgctgcgat cagcgcaccg accggcacct tgcggggcgt catgccctcg aggaactcgg
     3541 cggcgttgcg cggacgcccg gcgcgccgga acgacaatcc ccgctgcagc gtgcgcccct
     3601 gcgcccgcga cagcccggga atcggcgccg gcgccggctt catcttgcca ttgcgcgcgg
     3661 taatcgaatc ggtacgtagc aatttccccg gcttatcggg gtccggccgc tcgaacggat
     3721 gccggcccgt cagcagttcg taactgacga tggacaacgc gtagacgtca tcactgggat
     3781 ccggatcctg acgctcgagc tgctcgcagc ttgcgtacgg cagcgtcatc gcacccaggc
     3841 tgcccgcatc aaaccgggtc ttctcaccgt cgggcagctt ggtggcgcgc gcgatgccaa
     3901 agtccagcac cttcaccacg ccgtccttcg tcatgaaggc gttgctcggc ttgaagtccg
     3961 agtgcacgat gttgttggcg tgcgcgtagc tcaggcctcg tccgagctgc gtgatgatct
     4021 gcagcgcatc cttgaccggc agtccgccgc gatcgcgatg ggtgcgaatc acctcatcga
     4081 gcgagctgcc gatgagcagt tccatcgcca tgtagaccgt gtcgccatcc cggtcgaagt
     4141 cgtagaccgt gatgatgttg gggtgcgcca gggtctgggc gcggcgcgcc tcgcgctgca
     4201 gcgccatcat cgaatccgga tgctgcttga aatcctcgtt cagtaccttg atggcaacgt
     4261 acggattgcg gtcctgcgct tcctgcttga tgcggtcgag cgccttgaac accacgccca
     4321 tgccaccctt gtcgccgccg gcaacgaggg tttcgagcac gaagcgatct ttcagcaccg
     4381 ttccgacggt gacctgcgtg ccccagccac cggcggagga cgaacgcggt gaggaggccg
     4441 gtcgtggcga gggtgggctg ggtggcgcag cggcctgcgg tggcggtggc ggtggtggcg
     4501 gtggcggtgg cgagaccgcg gccgggggtg gcgggccgcg cacacttaaa tgtgttggct
     4561 catccggtgc cgtcgcggcc gtcggcaggc cggcactctg gcgtacgagc tgctgcagct
     4621 cggccgcgaa ctccggggtc agtctgcggc tggatcggta cgcctcgatg acggccagca
     4681 ttgcctgcgc gacctgcggt tcgctggcag ccgccgcgga tacacgtcgc cgggcctcat
     4741 ccgctcccag cgtccctgcc agaaactgtt cgagcgctgc tcgaaactcg ctcatcgatt
     4801 ccgatcctgt tccggttctt ccaacgactc gttcactgca atgcggatag gcactgaaaa
     4861 gtttacctgc tgactgccat ccgtgctcgc tttatgtccg caagttgaca caacgctcac
     4921 gcaaaatcgt ctaaggttga cgcatctgat gtgcgtctga gatcctgccc ctggctgata
     4981 ttttcccgcc acggcgaact gctttcagga agatcgacag tggccaacct atcaaaatca
     5041 ctctgctcga tgacggtttg cgccgccctg ttcgccaccg cggtccagtc ctcggatcat
     5101 agtgccgaaa cggccacgcg cgcgcagatt gccgcggcga gcttcgaaga caccattgtg
     5161 gttgactgtc agcttcctgg ccgcgtgcag cagctcggcg cggcgcgcac ctatctgacc
     5221 gcggggccgc tgatgcgact gcccgccatc gactgccgta cccgcggcgg tgagtacacg
     5281 atgggcgatc tggccagtgg cacgttgtcg ctcaaacgct ggctgccgct ggccaatcag
     5341 gacaacgtcg aagcgcagta ctacgtggcc cgcatctacg ccaacggcat ggacggcgtg
     5401 ccggtggact acgagctggc ggcgaagtgg tatcagcgcg ccgtgcagaa gaaatacgcg
     5461 cccgcgatgg aggaactcgg ttatctctat gagcaggggc tgggcgtgcc gaaggatccg
     5521 gtggccggga tcaacctgca gcgcgcagcc tccggactgg gtgaggatct ggtgtatgcc
     5581 tcgaccctgg cagcgaacaa ggaacaggcc gaagcccaga tcgcgctgct gaccgatgat
     5641 ctgaagagcg ccgatgcggt ggtcgtggat ctgcaggggc aactgactca ggccgatgac
     5701 aagctggcac agaatcactc gcagctgtcc gaagatcagg cacggctgcg ggacctgcgc
     5761 tccaagctcg agcaggcccg gcgcgatggc ggtacggccg atcttgccaa cgtgcagcgg
     5821 ctgcaacagc agctcgcggc cagcgaggcg gcgctcaagg acaagcagga tgcgatcaac
     5881 gttctgtccg ccgatcaggg tctgcgccag gcgcaactgt cagcgcagct ggccagatcc
     5941 caggcgacca acacccagtt gtccaacatg ctggccgccg gccagagcga gaatgagtcg
     6001 ctgcgagcga ggctggccca ggccgaacag cgtgcggtcc agtcgcagca ggaagtcgca
     6061 tcggtgcggg ccgatttcgt gcgcgagacc cgcgaactca gcgcccgtag tgcggaactg
     6121 acgcagctgc gtgagcacgg tagcgacggg ctgcagtcat tgctgtcctc caaacagggc
     6181 gaactggagc gtcaacagaa gctgctggcg tcgctcgaga gtcagctggc gagcctgcgt
     6241 cagcaggtga gcagcgcggg cagcggctcg agcgaagcga gcgcacgcaa caaggagctg
     6301 gaagccacgc tggcggcgct gcgcattcag tacgcgcagc agcagcagca gcttcaggac
     6361 cagcgcgcgg acttcgccag gctgcagtcg caatcgaagg atgaccgctc ggcgctgctg
     6421 gcgcgcctga gcgcccaact ggatcagaaa agcgccgagc tgcaggacaa gcagcaccgg
     6481 ctcgacagcc tgcaggccga atccagccgc ctgcgtgatg actacaatcg cgaacacgat
     6541 gcgcgtgcgc gcgatggcac gctcagcgcc agtcagctgc agcaggacaa agaggcactg
     6601 cgtgtggcgc aggatcagct cgcgcagcag cgcgccagcc tgcagcagct cgaactgcaa
     6661 tcggccacgc agcagctgca actggtgcag gagcgcgaga gattcgcgcg cgcgctggcc
     6721 ggtggccagc aggccgatca gcagaggatc gcgacgctgg aggcaggcgt gcgcgcgcgc
     6781 gaccagcaga tcgccgacgc ccgcgcgcgg gtcagtacac tggagcagca gaacagcgtc
     6841 gccaagaccg tcagcaccgc ccccgccaac aatgcggtga gcctcagcac gcctgtcagc
     6901 aagagcccgg tagacaccgg caccaccagc accgctagcg ccggcaccac cagcaccgtc
     6961 agctatcgcc tgccgcagag cgccgccgcc tcgggcaatc cgttcgacat ggttcagctg
     7021 gtgaagggtc tgggaccggc aaactactac gcgctggtga tcggcaatag caagtacgag
     7081 aagatgcagg cgctgaagac ggcggccaat gacgcgcgcg atgtcgccaa gctgctcgaa
     7141 gggcgctatg gcttccaggt aaagctgctg atcgatgcca cccgggccga catcggccgc
     7201 gcgctctacg aatacgcgcg cagcctgagc gattcggatc gactgctcat ctattacgcc
     7261 ggccatggcg gcagccgggt gtttccgccg gagcgcgctt actggacggg cgtggattcc
     7321 gaccctgaca atgcgagcag ctggatcggc gcgcaggacg tgagcgatga catcgcgcag
     7381 attcatgcgc gtcacatcct gctggtggcc gattcgtgct tctccagcgt gatcacccac
     7441 cagacctcca ccatcgtcgc gccctcgaac gatgagcgca gcgtcaggat caaatgggct
     7501 cgcagcgccc gcatggtgct gacctcagga cgcaacgagc cggtggtgga cagcgctgac
     7561 ccgcagcaga cgcattcgct gttcgccgag cagttcctga cggtgctgcg ccagaacacg
     7621 attctgctgt ccggccaggg gctggcgcat gagctcagcg gccgcgtgga ggatgcggcg
     7681 gctcgaacgg gcctgaagca gacgccgacg tactccaacc tgcaggatca gcagcacatg
     7741 ttcggcgact tcttcttcgt gccgctgacc agcgtgcagg tggcctctac ggcgcgttga
     7801 gcgccatctc ctcatccaga tgcttgagga tgtccggcca atgccggatc cagcagcaga
     7861 cgtgatcgaa gcccggaaag cgccagagtt gactcgcctg cagtctctgg aaatagcgta
     7921 cggttgaacg ctccggcacc gtgaggtcgc gaccgccgaa cagatggcgt tgcgcgatcg
     7981 cgctcggcag cgccggttgc gtggccggat ccaggctctg atcaagcggc aggtagccat
     8041 gcactcgcgc ccactcgccc acgtcaaggt tggctgcaat ggtgatgatc gcgcgcgttg
     8101 agggcacctt cggcgccatc agaaccgcca gcgccccgcc accgctatag ccgatcagta
     8161 ccagatgctg atagtccccg gcggccgcaa atccattggc cgcaactgcc atgctgtcca
     8221 cgacctgcgc ggaatagcgc ccggaggtcc aaacacccgg cgtgcatccg gcgcggtcgt
     8281 ggactgagaa ataacagggg cggcccagat agagcaccgg gtgcgcggtg cgcgcggcca
     8341 gggccagcgc cagcgggtgt aagggggtcg gatcgctcgc gaccctcgtg ccatgcccgc
     8401 tccagggcac accatccccg tcgatgaaca catagagcac tttgccgtca ggatgcgatg
     8461 accagaacac ctggtgctca aaggcctcac cctggatgag cgagtcggtc agggcatgct
     8521 ggctcgcaaa gtcacgcgcg cgctgcgtcg cactcgcgca cgcgcccagc agcaggcacc
     8581 acagcgtgac gttaagggac gaccagcgga taggtcgccg tcgtgctcgc accgctcacg
     8641 ctgccttgcg ccgtcaccga cagatagtag gtaccggaaa caggggttgc gaaagcgggg
     8701 aatgccgctc cggcctcggc ggcaccggca ctgctgcccg ccggtacgat cggtgttccg
     8761 atcagaccta acagggatcc ggggcatgcg ccgcctgggc tcgccggcgg tccgccggag
     8821 cacagcgacc actgatagct accgatgaag acactctggg acgccgcatc gagcaggatg
     8881 cccgcaacgg cggccagtgt gttgctggca ttcggactgg cttccgtact gaccagcgcg
     8941 gctctgccgt tgagcgtcac gagctgcggg gctccgggac cgatgacatt ctgcgcgctg
     9001 tccaccgcaa ccggtgtcgc acccgggagc gaattgaggt aggtagccag cgtctgcgcg
     9061 gcactctgcc cgccgccgaa gtcaacccag aatcgatcgg cggtcaggcg cgattgcggc
     9121 atcgtgccct gggtgaacac cagcgactgc aggaacgccg gcaagccggg cgccagcggt
     9181 tggtcgagga acaggaacga cgccagcgac tgaaactgca cgtttccgat ctgcgctccg
     9241 ccgttgacct gcgtgtggca ggcgcggcag taatgccgga acacggcgtg atacagatcg
     9301 ttcgaggcgc ccgtgctctg accggccgtc agccatgcat cgggtgtggc cgaatcatcg
     9361 aatgggtgcg ccgtagaggc tgccggatcg ccgccatacc atttgctcat cagcgcgcgc
     9421 gctgccgcgt agcgatcgac gctcgcaccg ctccccgtcg tgatcatctc cggggccgta
     9481 taggtgcgcc acgccagagc gttgagctgc tgaatgcgtg gctgctgcgc cgactgggtg
     9541 aacttgctcg catcgaccgc accgcacacc ggcgagagcg tggacacctg gcaggggaaa
     9601 gccgggtcgg tgtcggcaaa cagcatcgcg ccgacatccc acggcatgaa ggtcgcatca
     9661 acgtcgcccg ccgccggata gggctgaccg ggcgtaaatc cgggcgcacc tccatgacag
     9721 gcggtgcagg cgccgggcag atagcgctgt ccgcgacgat cgaaattggc cgacagcacc
     9781 cgctggaaaa cgccggtgcg atcatccggc gcgaaggcgt agaacttggc gtaacacgcc
     9841 cccgtattgg tggtcgaatc cgccgggtta gtgaagcatg gcgccgtgga acccggcgcc
     9901 ggcgtgtact ccatcgccac cgccaggaag gcgccgatgc ggttcaccgc cgcgcccagc
     9961 gacggatagt tcacgaccac ggaggccatg ttgccgttgg tgcagttggc gaaatacatg
    10021 tcgcggccga acgcgagatc gaaattgttg gcgtagtccg cgtgcgtgac cgagaagccg
    10081 gcctcgccct cgccgtagtt gcggtcgttc ggatcgaagc agttctgctg cagccagccc
    10141 tgcagcgtgc tgcgctgcga attcggatcg atcgccgcgt agtaagcgcc cgccgactcg
    10201 cgcgtttcaa acgacgacgg cggtccgcct gcggtcagtg cctgggcgcg cagaaaatct
    10261 tccggcgtca gactggccga gccgtccggg gtcagcggca ggctggtcag cggggtgtcg
    10321 atggtcacgg cagttcccgc agtgctggcg gtggccggcg tgccgagcgc atcgccggcc
    10381 tcgtcgtaga gctgcagcat cagctgcgag gccgacaggg cgctgccggc ttgcgatccg
    10441 tcacaggaac cgggcgtcgc gctgaccgcc atgcgtacga acgccgtatc gacgtccgaa
    10501 tccaccagct gattgttgtt gaggtttgcc cccggtgcgt tgtaagcgac aaacacgtct
    10561 tcgctgttca gcacgggcag cgggaacgac accaacggat tgctgaagga gagcggtgtc
    10621 gaaaatcctg gcgccgccgc gactccagcg acccacttgg catcgatctg cttcgtcggc
    10681 agcgtgacgg ccccggattc ggcgccaccc tggcgcgggg agtagatgac ggtacgggtc
    10741 agctgcacac acaccggtac atcggcggtc ggaccggcaa gaccggcgac cgtgctgacg
    10801 gccacccgga acgtgtgcgg cagcagcggc ggcgctgcgg cctgagtgtt gaggaaacga
    10861 ttgaggtcgt tggccggcac cacctgcacc tgcgcggtgg cggtgccggt gaggcccgat
    10921 gcattggtga cgctgagctg aaacttcaga gtcgcgctct gcgagagcga cggggcggtg
    10981 aatgagacgg tgctcgagtt gcggtacaga agagtgaccg ccggtccggc ggtctgcgtc
    11041 caaccaaaac ccgtgatcga aacattgcca ccgtagctgt cctcgccgga caactgcaca
    11101 tccgctcccg cgcgtactgt gatcgacact gagccggcgc tggagccgcg agagggctga
    11161 ttgccgatgg tcgcaatcag tgcggtgggt gtctgcacga actcactgct gctgctgcca
    11221 ccacaggccg ccaccagcgc ggtcgccaga tacaccgtca cgcggacgag acgttggaat
    11281 tgccttttca tcgatgccac ctggagcggg cacgcctgct tcagaacgtg aagcggaaac
    11341 cacctgagat tgcgtagtaa ctgtagtact gcagctgcag caccttcaga tactgcgcat
    11401 agagctgaaa gcgatgcggt aattcggtga tcaccccgcc accgacttcc caatagttcg
    11461 ggtcgatgtt gtcggtcgga agctggaact gatcggcgac cgattgcgac gaaacatagt
    11521 gcgagctgac gctctgcggc ttgtcgcgga actcgtgacg atattcgccg tagccgtagg
    11581 gaatgatcac gccgagcctg gtggaccagg cgtaattgaa cttcagcccc agaatgctct
    11641 gcaacgaggt gacactctgg ccattcaccg ataacgccag ctccgggtcg gagcccgagc
    11701 cggtttcagt gaaggcgccg atgcgcgtgc ccagatactg cagattggcg tacggcgtgg
    11761 cggaaaaggc gtgcagatac caggagtagc ccccgcccac ggtccccagc cacgaggtcg
    11821 cgccggtgct gctgaagaaa ttggtattga ccgagggctc ctgcggattg ttcgacgggt
    11881 aggccaccag gcggcgcgtg tcgagggtca tgtgctgcac gcccaccgac acattcagat
    11941 acgcgctgtt ccagtcctgc tcgaaataaa cggtggcccc gccgccgctg cctttgacat
    12001 tgccgctggc aatgctttcg cttgaattga aattcgcgtg ctgcgacacc tcgctgccga
    12061 tgattccgaa cacgctgtgg gcggagagtc gcacgtcggc accgaacaga aactgctgtc
    12121 cggagaaatc gaaggcatcg ttgaaggtcg tcggcgcctt gctgcccgag ccataagaac
    12181 cgctcgcgaa cacgctccag cgtccgacac cgggaagatt ctcatcggcg ctcgcgccgc
    12241 cgccgagcgc ttgctcggca tcggcatccg ccagctgttc atcgtcctga tagccctggc
    12301 gcgcgaagcg catcgtctgc gtgatgaagc gcaccgcgtt cagtcggttc gtgacggcat
    12361 cgaactgatt gctcgccagg cgattggtga tcgaggactg cgcggaatac tcctcgtcgg
    12421 cggtccagcg cagcgcgaat cccagtgcct gggccgacag atgcagactg aatgccgatt
    12481 gcccgaaacc cagcagctcg gccgcgttgt cctccagctc gcgcagctgg atgaaaaggt
    12541 tcagctgcgc gccgctgcaa ccgctgccgg tgagaccgca cagcttcagc aggctgacat
    12601 aggccgcatc gtcggcctgc gcggccgcca gttcgagccc gttgaaattg aatgcattgt
    12661 cggtcgtgac cagcgtggtc agacacggcg ccggcacggc gccgtcgccc gcgacgccga
    12721 tggtgcagcc ggacccgccg ttgggagcgg gcgcggtctg cgcccacgca ctagcgccta
    12781 cgatctgcag cagcagcgcg ccgcacgcga gcgaaagccc gcgacgcacg cctcgcgtac
    12841 caaaatcttg cctttgggac tggttgttgc gcattgcgcc agcttccctg ctacggattt
    12901 gacggccaca cggctgggcg tgccgacggt gctcgttcgt tgttaccgga ttagcttaca
    12961 caacgtaaag cgtaacgagt agagcttttg cgacatccac caacgatttt tgggacttcc
    13021 cgagcgttgc aaattctgcg cgtcaaccat cggaaagtca ttcggcccgg cagatcttcg
    13081 gatcgcggcg gcaaatgccc tgcttgagtc gcagcagctg gaggttgtgc tcgaagtttt
    13141 gtccgtcggt cacgagccgt agcgcctgcg cagtgtcgtt ttgctgcagt cgagcgcggg
    13201 caaggcccgc ataggcacgt gctgcctggc taataatctg ttttgcctga gcattgtcag
    13261 gattgcactg caggacctgg gtcaacatgt catgcaggtt gttggcaccc ttggaaagca
    13321 aggcctcgac ctcgtcggtc gacgccatgc tggaggcctg cttgagcgca tccgcggaca
    13381 ggaacagcgt atcgccaatc tgacggcact gcgcttcggt gaccggctcg ggcgcggaaa
    13441 ccgcgggagg ccacagctgc gcgttgagcg cctggtcctg cacatactca ctgtagaaac
    13501 gcgcgcccag gaacagcgcc atggccagta ccgtcgcgac cagggctccg atccacaacc
    13561 tgttcttctg cagccaggtc tgcggggcca gcaggcgcag gaactcgctg acgctctcga
    13621 tgcggtccgc gcgccgcagg gccaggccgc gccgcacgcc gtcccactga cgtcgcgaca
    13681 gggaatcgat gcgctggggt accagctggg cttcgaatgc ggtacgtgcc gaggcgcgtg
    13741 caaacggatg cttgccaccg aaaataaaat acgcgacgca ggcaaacgca tagatatcgt
    13801 cgcgcggatc cggtgggtcg cgattccact gctcgagtgc agca
//
LOCUS       DATMRM010000332         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_821834, whole genome shotgun sequence.
ACCESSION   DATMRM010000332 DATMRM010000000
VERSION     DATMRM010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821834"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..1113)
                     /locus_tag="VNZ06_09910"
     CDS             complement(274..1113)
                     /locus_tag="VNZ06_09910"
                     /inference="COORDINATES: protein motif:HMM:NF005065.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWW21107.1"
                     /translation="MIKTWFITGISSGLGRNMTEQLLARGDRVAGTARRLHALDGLVS
                     RYVTQLSVAALDVTDSAAVRRVVDGAFNSLGRIDVIVNNAGYGLFGAAEELNDEQMRQ
                     QIDTNLVGSIQVIRAVLPHLRAQGGGRILQVSSEGGQIAYPGFSLYHATKWGIEGFVE
                     AVSQEVAPFNIEFTIIEPGPTATDFGKGMISTPATAVYESTPVGEIRRAWSSGRFSVR
                     GDARKTARAMIDCADQRSGPGRLTLGSDAYERVHAALTARLTVLQGQKATARSTDLDP
                     LDR"
     gene            complement(1137..2018)
                     /locus_tag="VNZ06_09915"
     CDS             complement(1137..2018)
                     /locus_tag="VNZ06_09915"
                     /inference="COORDINATES: protein motif:HMM:NF007695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HWW21108.1"
                     /translation="MSSINRTGTFALGDRTVKRLGYGAMQLAGPGVFGPPRNTDAALT
                     VLREAVASGVNHIDTSGYYGPHITNQLIRRALHPYRDDLVIVTKIGARRGTDGSWLQA
                     FSSQELTQAVHDNLRNLGLDVLDVVNLRSMLGVHGPAEGSLAAPLSVLAELQRKGLVR
                     HIGLSNVTPAQIEEGRRIVKIVCVQNHYNLAHRADDALIDDLAINGIAYVPFFPLGGF
                     SPLQSSILSDVAQRLGATAMQVALAWLLRRAPNILLIPGTSSVAHLRENLAVAELDLP
                     NDAVVALDGIGAHADAA"
BASE COUNT          416 a          663 c          644 g          410 t
ORIGIN      
        1 cgacgcaagg gacagcaatc ttggcggtgg catctcccac ctccattggt agcgtcggct
       61 tccgtacccg atgtgaaaag tttggctaca cctcacctag aatcaatcca gaaatatcca
      121 gtaaaatgaa tgcgtcccaa cggctgcgtt tgacctcgag ctgaaattgg gaagccagaa
      181 cgttgacctg gcgcagacga cctgtatgcg atcggcgacg ctgccggcac ccatgggttg
      241 cgtacgcgcg tgcccgggat ggggcagtct gatctaacgg tccaatggat cgagatctgt
      301 tgagcgtgcg gtcgctttct gaccttgcag cacggtgagt ctcgctgtca gcgctgcatg
      361 aactcgttcg tacgcgtcgc ttcccaacgt gagccgtccg ggcccggagc gctgatcggc
      421 gcaatcgatc atcgcacgtg ctgtcttgcg ggcatctcca cgcacggaga acctgcctga
      481 gctccacgcc ctgcgaatct cgccgactgg cgtgctctcg tagaccgcag ttgcgggcgt
      541 gctgatcatg cctttgccga agtccgtggc cgtggggccc ggttcgatga tggtgaactc
      601 gatgttgaac ggtgccacct cctgcgacac cgcctcgaca aacccctcaa tgccccactt
      661 cgttgcgtgg tagaggctaa agcccggata ggcgatctgc ccgccctccg aggacacctg
      721 cagaatgcgc ccgccgccct gtgcccgcag gtgcggaagt acggcgcgga tcacctgaat
      781 cgaaccgacg aggttggtat cgatctgttg cctcatctgc tcatcattca gctcttctgc
      841 ggcgccaaac agtccgtagc cggcgttgtt cacgatgacg tcgatacgcc ccaaagagtt
      901 aaaggctcca tcgaccaccc gccgaacagc cgcactgtcc gtgacgtcca gcgccgcaac
      961 gctgagctga gtcacatagc ggctcacgag tccatcgagc gcgtgcagtc tgcgcgccgt
     1021 cccggcgact cgatcaccgc gggccagaag ctgctccgtc atgttccgtc ccagaccgga
     1081 ggagattccg gtgatgaacc acgtcttgat catcggaagc gctcctctta ctggcgtcag
     1141 gcggcgtcag cgtgcgcgcc aatgccgtct aaggccacga ccgcatcgtt gggcagatcc
     1201 aattcggcca cagccaggtt ttcgcgcaag tgcgcgaccg aggaggtacc cgggattagg
     1261 agaatgttgg gcgcgcggcg aagtagccat gcgagggcaa cttgcatggc cgtggcaccg
     1321 aggcgctgag ccacatcgga caagatggaa gactgcagcg ggctgaagcc gcccaaggga
     1381 aagaacggca cataggctat gccgttgatg gcaagatcat cgatgagtgc atcatctgcc
     1441 cgatgtgcca agttgtagtg gttctgcacg cagacgatct taacaatccg ccgcccttcc
     1501 tcgatctgcg caggcgtgac attgctcagt ccgatatgac gaaccagccc cttgcgctgg
     1561 agctcggcca gtacgctgag cggcgccgcg agtgagcctt cggcaggccc atggacaccc
     1621 aacatgctgc gaagattcac cacatcgagc acatccagac ccaggttgcg caggttgtcg
     1681 tgcaccgcct gggtcagctc ttgcgaagag aaagcctgta gccacgaccc gtccgtgccg
     1741 cgccgggcgc cgatcttggt cacgatgacg agatcatcgc gatatggatg cagggccctg
     1801 cgaatcagct gattcgtgat atgcgggccg tagtaaccac tggtgtcgat gtgattcaca
     1861 ccgcttgcca cggcttcgcg caggacggtc agtgcggcat ccgtgttcct gggcggcccg
     1921 aacacgccgg gccccgcgag ctgcatggcg ccgtagccga gtctctttac ggttcggtca
     1981 cccagggcaa atgtgccggt tctgttgatg ctggacatgg tcgatctcct ttcgagcagg
     2041 gacacaatct agccgctgtc ggcctgcgcg ataatagggc ccaatccgca cgggccgtgc
     2101 ggacaggcga tcagtgaaaa ctgaatctcg ctg
//
LOCUS       DATMRM010000333         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2042565, whole genome shotgun sequence.
ACCESSION   DATMRM010000333 DATMRM010000000
VERSION     DATMRM010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2042565"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VNZ06_09920"
     CDS             <1..309
                     /locus_tag="VNZ06_09920"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWW21109.1"
                     /translation="SKIGMNMLGQQLALSNAKYGIRVNTIMPGLLDTPMSIESTARAR
                     KISHSELRSERDRRVPLKGKMGTAWDVAHAALFLHSEESRFITGIVLPVDGGQSARIG
                     "
     gene            775..1197
                     /locus_tag="VNZ06_09925"
     CDS             775..1197
                     /locus_tag="VNZ06_09925"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HWW21110.1"
                     /translation="MSASSHAAADDVYKTVDGQGNVVYSDHAIAPTSKKVTVDVIQGN
                     AEDAARLAKQRALANADAAQQAKIAQQQAAEQQQQQAQEALKKRKCEAARNRYALFAA
                     GGRIFRTDEQGNRAYYSDAEIDAQRITTKAAMDAACSQ"
     gene            1215..2132
                     /locus_tag="VNZ06_09930"
     CDS             1215..2132
                     /locus_tag="VNZ06_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWW21111.1"
                     /translation="MSLAAGLLLIQINSAADELYKYHDANGNLVYTDRKPTSPAATIE
                     PLSVGLESKAPRIEIQAVSDDTKWRLVATNECLCVVEFGGEITEAHNLNLADGVTFHQ
                     ILAPRSQQALLEVAHSPRSGTPMARVAWKGILGAPGAEHSPPMPYRAPFALGASYKVT
                     QAYPVRLTHASAADQYAVDFALPDGTPVNAAREGTVINVKHDKYMGAATPLMMDQANM
                     VEILHDDGTIAIYAHLHWDSVRVQPGQHVRRGEYIADSGSTGFSTGAHLHFAVVRNTG
                     MSTESVPVQFAGPGGTAITAQTDALLTAY"
     gene            complement(2137..>2656)
                     /locus_tag="VNZ06_09935"
     CDS             complement(2137..>2656)
                     /locus_tag="VNZ06_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HWW21112.1"
                     /translation="VRRVVGMVFQSFNLFEHLTALENCMLAPMRVRRLPRKLAEAISL
                     DYLGRVHMLDFVSSYPAQLSGGQQQRVAIARALCMQPRVMMFDEPTSALDPEMTKDVL
                     DIIVGLSTTGMTMICVTHEMSFARQIADRVIFMDRGQIVEEGSPTDLFERPKTERLKN
                     FLNQVLHERQRQ"
BASE COUNT          588 a          819 c          760 g          489 t
ORIGIN      
        1 tcgaagatcg gcatgaacat gctgggccag cagctcgcac tgtcgaatgc gaagtatggc
       61 attcgggtca acaccatcat gcccggtctg cttgacacgc cgatgtcgat cgagagtaca
      121 gcacgagcgc gaaaaatctc acactcagaa ctgcgctccg agcgcgacag acgggtgccg
      181 ctcaagggca agatgggaac cgcatgggac gtggcgcacg ccgctctctt tctacattcc
      241 gaagaatcgc gcttcatcac aggaatcgtg ctacccgtag acggtggaca gagcgcgcgc
      301 atcggctagg gccttcgctt gtgtcacgct catgacggaa cttcgtcacg cactggcgca
      361 cccttcgctc ccagtgacca gctagccaac ggtcgatcct atgaagaaaa agttacgctc
      421 accaatcgcg gcactcagca cctgttcgcg ataacgtttg cgggtgcggc ctagccacca
      481 gcccgtcggt cccggcggcg ccagggtgcc tgatgccgct acaggtggac cgacttcgca
      541 tcggcagccg cgcccgcccc attgactagc gaccagcgga cactgcgatg ccagctcttt
      601 tggccctcga tggcagtcga tcaaaaccag tacacttccg tccacgaatt acttgccctg
      661 gatcacacat tgcagccagc cacggggcgt gataacgcct aaaatcagtt ccggtccgaa
      721 ggcacagata tgacacccgt aagctggttt gcgagaatcg gcgccgtact catcatgagc
      781 gcgagcagcc atgccgctgc cgacgacgtc tataagactg tggatgggca gggcaacgtc
      841 gtctattcgg accatgcgat tgcgccgaca tcgaagaagg tcactgtcga cgtgattcaa
      901 ggaaatgccg aagacgccgc gcggcttgcc aaacagcgtg cgcttgccaa cgcggatgcc
      961 gctcagcaag ccaaaatcgc tcaacagcag gccgcggaac aacagcagca acaagcgcag
     1021 gaagcgctga agaagcgcaa atgcgaagcc gctcgcaacc gctatgcgct cttcgccgcc
     1081 ggtggacgca tattcagaac ggacgaacag ggcaatcgcg cctactactc cgacgccgaa
     1141 attgacgcgc agcgcatcac taccaaggcg gcaatggacg cggcgtgttc ccagtgagtc
     1201 gagttcgctt ggtgttgtcg ctcgcggccg gcctgctcct gattcagatc aattcggctg
     1261 cagacgagct gtacaagtac cacgacgcga acggcaatct ggtttacaca gatcgcaagc
     1321 ccacctcccc tgccgcgacg attgagccgc tttcggtcgg cctggagagc aaggcgccgc
     1381 ggatcgaaat acaggccgtc agcgatgaca ccaaatggcg attggttgca acaaacgagt
     1441 gtctgtgcgt ggtcgaattc ggtggtgaga tcaccgaggc gcataacctg aacctcgccg
     1501 acggggttac gtttcaccaa atactggcgc cgcgttcgca acaggccctg ctcgaagtcg
     1561 cacattcgcc gcgcagcggc acaccgatgg cgcgcgtggc ctggaaaggc atccttggag
     1621 cacccggagc cgagcacagt cctccgatgc cttatcgcgc cccgttcgcc ctgggcgcaa
     1681 gttacaaggt gacccaggcc tatcctgtgc gcctcaccca cgcgagcgct gccgaccaat
     1741 acgccgttga tttcgcgctc ccggacggca ctccggtgaa cgccgcacgt gaaggcaccg
     1801 ttatcaacgt aaagcacgac aaatatatgg gtgcggcaac acccctgatg atggatcaag
     1861 caaatatggt ggagatcctg cacgatgacg gcacgatcgc catctacgcg catctgcact
     1921 gggattcggt gcgcgtgcag ccgggacaac atgtacgacg cggcgaatat attgccgact
     1981 cgggcagcac ggggtttagt accggcgcac atctgcattt tgcggtagtc agaaacacgg
     2041 ggatgtccac tgaatcagta ccggttcagt ttgccggccc cggcggaaca gcaatcaccg
     2101 cgcaaacgga cgcgctgctc acagcctact aggctttcac tggcgctgcc gctcgtgcag
     2161 cacctggttc aggaaatttt tcaatcgctc ggttttcggt cgctcgaaca agtcggtcgg
     2221 acttccctct tcgacaatct gaccgcggtc catgaagatt acgcgatcgg caatttgtcg
     2281 tgcgaatgac atctcatgcg tcacgcagat catggtcata cctgtggtac tcaggccgac
     2341 gatgatatcc agcacgtctt tggtcatctc cgggtcgagg gccgaagtcg gctcgtcgaa
     2401 catcatgacg cgtggctgca tgcacagggc gcgcgcgatg gcaacgcgtt gctgttgacc
     2461 acctgacaat tgcgccgggt agctgctcac gaagtcgagc atatgcacac ggcctagata
     2521 gtccagcgag attgcttcgg ctagttttct tggcaggcgg cgcacccgca tcggcgccag
     2581 catacagttc tcgagtgccg tcaggtgctc aaacagattg aaggactgaa acaccattcc
     2641 tacgacgcga cgcacg
//
LOCUS       DATMRM010000334         7095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2672841, whole genome shotgun sequence.
ACCESSION   DATMRM010000334 DATMRM010000000
VERSION     DATMRM010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2672841"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VNZ06_09940"
     CDS             complement(<1..1028)
                     /locus_tag="VNZ06_09940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWW21113.1"
                     /translation="MLRNPVRTAVAALIAMSALSGCTLEPHYKRPDLPVPEQWPLPEN
                     STPMPSADAGATGASQTSVWEIGWREFFTDPQLQQLIAQALANNRDVRVAVLNVELAR
                     AQYHVQRANLLPKVDANGSYTREKIPPALAFGTQLPPGTTIPLEKIYQVGVGVSSWEI
                     DLFGRVRSQSHAALQQYLAQEQARRSAQLSLIAQVAGAYLTLAADRELDRLALETLKG
                     QEASYDLTRQRHESGAVSGLDLAQARTTVEQARVDAARFEGNVAQDVDALALLIGGAV
                     DAGTLPRELDANITGLAPLPPALPSTVLLRRPDILQAEHQLRAANADIGAARAAFFPS
                     INLTGAIGS"
     gene            complement(1028..4207)
                     /locus_tag="VNZ06_09945"
     CDS             complement(1028..4207)
                     /locus_tag="VNZ06_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW21114.1"
                     /translation="MSNFFIDRPIFAWVIAIILMMAGIVSIVRLPVARYPQIAPPSIV
                     VNANYPGASAQTVQDAVTQIIEQNMIGLDGLLYMTSSSDSAGSIAITMTFGSGTNPDI
                     AQVQVQNKLQSAVPLLPQIVQQQGITVTKTNSNFVLAIGFVSDNPSLTTADVNDYLAT
                     NIAEPLSRIDGVGTVQLFGSGKYAMRIWLDPNKLATYSLMPEDIVTAIKAQNAQISVG
                     QLGGLPYVPGQQLNATVTALGRLSTPEQFRNIILRSNTDGSTLRLGDVARVELGASSY
                     GAEARFDGQPASGMGISLTSGANSLKTAAAVEEALQRFQPGFPAGLRYAVAYDTTPFV
                     RKSITEVVKTLTEAIVLVFLVMWLFLQNLRATLIPTIAVPVVLLGTFAVLKAAGFSIN
                     MLTMFAMVLAIGLLVDDAIVVVENVERLMRDEGLSPRDATRKSMQQITGALIGIATVL
                     TAVFIPMAFLNGSSGVIYRQFSLTIVAAMVLSVLMAIVVTPALCATLLKPVAKGEHHS
                     NRGLFRWFNQFFDRSNLRYQSTVARILSRPARFLLVYAALASIMGMLFFRLPTAYVPE
                     EDQGYLFTVVQTPVGATLQRTLNAMEKIKDYFLTQEKDAIEGSFLVAGFSPTGAGQNV
                     GFGYIVLKDWSLRKSSRLSLSAIQGRAYGALAAIKDAQAFVFAPPAINELGNSAGFDF
                     YLKDNKGKGHEALIDARDQFLRTARKDPLLANVRPAGVEDAPQFRLDINSQKVISLGL
                     SMDDVNDTLQIAWGGRYIDDFIDRGRVKHVLIQGDAPYRMDPEDANTWYVRNKVGSMV
                     PVASFASSHWEYGPPRLERFNGVSAVEINGQPAPGVSSGEAMKEIEKLVGQLPQGYSV
                     DWTGESYQERAAGAQTPALYTLSIIVVFLCLAALYESWSIPTAILMAVPLGVIGAVLT
                     TTARGLERDIYFQVGMLTTIGLTSKNAILIVEFAKEYVEAGMNTIEATLHAVRDRLRP
                     ILMTSLAFGLGVFPLAVATGAGAGAQRAIGTGVFGGMLGGTFLGIFFIPLFFVIVQRA
                     FHGVMEHAPTHAAENPSLMPPPVSPARGG"
     gene            complement(4216..5415)
                     /locus_tag="VNZ06_09950"
     CDS             complement(4216..5415)
                     /locus_tag="VNZ06_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWW21115.1"
                     /translation="MPALPTVSGRPSFVVAALTFGLLLAGCGQNSARQYARPAQQVTI
                     VTLKPQPVPLTRELPGRTAAYLVAEVRPQVSGIVKQRLFTEGAFVKTGQPLYELDDAP
                     YRAQYNNAKAALQKAEATVEAAGTTARRDADLVKIDAVSQQDNDNAVAAWRQAQADVV
                     AAQAAVDSAAVNLAYAHIVSPITGRIGKSSVTQGALVTQNQTDAMATVQQLDPIYVDV
                     NQSSSEWLQLRQEVDSGRVQGGGAGAAAKIMLENGTEYGHEGKLQFADVTVDPTTGEL
                     LLRAIVPNPDHILMPGMYVRAIIREGVVTNGLLAPQQGIARDPRGNATALVVNAQNKV
                     EAREVRVARTIGDQWLVEGGLSAGDRLIVEGVQKVQPGMQVQTVEQAVAGASGETAPV
                     AVATAQR"
     gene            complement(5646..5990)
                     /locus_tag="VNZ06_09955"
     CDS             complement(5646..5990)
                     /locus_tag="VNZ06_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21116.1"
                     /translation="MMTRTFKMAAIAAVIAAVPTIGAATDRPRALNGCVKAFMAELST
                     KMPNTLKLLNTRYLDEAGASNLAALASNSELELTARDAHDNRAVARVICTVNSQGEVL
                     DLRAASLGYEPF"
     gene            complement(6202..>7095)
                     /locus_tag="VNZ06_09960"
     CDS             complement(6202..>7095)
                     /locus_tag="VNZ06_09960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW21117.1"
                     /translation="SWLNDRASSANRGRLLAVYMAVLYVGLGVAQLLLMLANPRSPFP
                     FMLISALISLAMVPIVVSAQKMPECSLPRPVRYRELYRNSPLGVVAVTVSGLISAILF
                     SMGPVYARLSGLGVAGVATFMSVSILAAVVTQYPVGRLSDRIDRRTVIAGVCTIATLV
                     AGSIAVFSGMPRALFLMLAAVFSGSVLTLYSLAISHVNDKLEPAQMVAASSALLLLNG
                     AAAAVGPLLMGSLMAAFGPSVYFVTLASLTAALTIYDLWRKSRRKPVPRTQKHGFISV
                     QPQGMTAQVVTAPNMTRTASN"
BASE COUNT         1411 a         2229 c         2115 g         1340 t
ORIGIN      
        1 gagccaatcg cacccgtcag attgatgctc gggaagaacg cagcacgtgc cgcaccgatg
       61 tcggcattcg cagcgcgcaa ttgatgctcc gcctgtagaa tatccggccg ccgcagcaat
      121 accgtggacg gcagagccgg cggcaacggt gccagcccag tgatgttggc atccagctcc
      181 cgcggaagcg ttccggcgtc gacggctccg ccaatcagaa gcgccagcgc gtccacgtcc
      241 tgcgccacat tgccctcaaa gcgcgccgca tcgacgcgtg cctgctccac tgtggttcgg
      301 gcctgcgcca gatccagacc tgagaccgca cccgactcgt gacgctgcct cgtcagatcg
      361 tacgaggctt cctggccctt gagtgtctcg agcgcaagtc ggtcgagttc acggtccgcg
      421 gccagtgtca ggtaggcgcc cgccacctgg gcgatcagac tgagctgcgc actgcgccgc
      481 gcctgctcct gcgcaagata ttgctgcaac gctgcgtgcg actgactccg cacacgtcca
      541 aacagatcga tttcccatga gctcactccg accccgactt gatagatctt ctcaagcgga
      601 atggtcgtcc cgggtggcag ctgcgtgcca aaggccagtg cgggcggtat cttctcgcgg
      661 gtataggaac cattcgcgtc gaccttcggc aacagattgg cgcgttgcac gtgatactgc
      721 gcgcgtgcca gctcgacgtt gagcaccgct actcgcacgt cacggttgtt cgccagggcc
      781 tgcgcgatca gctgttgcag ctgtggatcc gtgaaaaact cccgccagcc gatttcccag
      841 accgatgtct gactggcacc ggtggcgccg gcatcggcag agggcatcgg cgtgctgttc
      901 tcgggcaggg gccactgttc cggcaccggc agatccggac gcttgtagtg cggctccagt
      961 gtgcagccgg acaacgcact catcgcaatg agtgcggcca ccgcggtgcg aaccgggtta
     1021 cgcaacatca gccaccccgc gccggagaga ccggcggcgg catcagtgaa ggattctccg
     1081 cagcgtgcgt gggtgcatgc tccatcacgc cgtggaatgc gcgctgcacg atgacgaaga
     1141 acagtgggat gaagaagatt ccgaggaacg taccgccgag catgcctccg aatacgccgg
     1201 tgccgatcgc tcgttgcgca ccggcgcccg caccggttgc cacggcgaga ggaaacacgc
     1261 ccaaaccaaa ggccagcgaa gtcatgagaa tcggtcgcaa gcgatcgcgc accgcatgca
     1321 gcgtcgcttc gatcgtattc atgcccgctt caacgtattc ctttgcgaat tcgacgatca
     1381 gaatcgcgtt cttgctggtg agtccaatgg tcgtgagcat cccgacctga aagtagatat
     1441 cgcgctccag accgcgcgcg gtggtggtga gcaccgcgcc gatgacaccc aacggtacgg
     1501 ccatcaggat cgcggttggt atcgaccagc tctcatagag cgccgccaga cacaggaaca
     1561 cgacgatgat cgacagtgtg tacaaggcgg gagtttgtgc tccggccgcg cgctcctgat
     1621 aggactctcc ggtccagtcc accgaatagc cctgtggcag ttgaccgacc agcttctcga
     1681 tctccttcat cgcctcgcca gaactcacgc caggagccgg ctggccgttg atttctaccg
     1741 ccgatacgcc attgaatcgc tccaggcgtg gcggtccgta ctcccaatgt gagctcgcaa
     1801 atgaggcgac tggcaccatg ctgcccacct tgttgcgcac ataccaggtg ttagcgtctt
     1861 cgggatccat gcgatacggt gcgtcgccct gaatcagaac gtgcttgacg cgaccgcgat
     1921 cgatgaagtc atcgatgtag cgaccacccc aggcaatctg caatgtgtcg ttgacatcgt
     1981 ccatcgatag acccagcgag atcactttct gtgaattgat atcgagccga aattgcggtg
     2041 catcctcgac tcccgccggt cgcacgtttg ccagcagcgg gtccttcctg gccgttctca
     2101 ggaactggtc gcgagcgtca atcagggcct catgtccctt gcccttgtta tccttcaaat
     2161 agaaatcgaa gccggcggag tttccaagtt cgttgatcgc gggcggcgca aacacaaagg
     2221 cctgcgcgtc cttgattgcc gccagcgccc cataggctcg gccctggatg gccgagaggc
     2281 tcagcctgct cgactttcgc aagctccagt ccttgagcac gatatagccg aagccgacgt
     2341 tctgcccggc tcccgtaggg ctgaaccctg ccaccaggaa ggagccttcg attgcatcct
     2401 tctcctgcgt cagaaagtag tccttgatct tttccatcgc gttgagcgta cgctgcagcg
     2461 tcgccccgac cggcgtctgc actaccgtga acagataacc ctggtcctcc tcgggcacgt
     2521 acgcggttgg caagcgaaag aacagcattc ccataatgga cgccagggct gcgtacacca
     2581 gcaggaaccg tgcgggacgg ctcaggatgc gcgcaaccgt actctggtag cgcaggttgc
     2641 tgcgatcgaa gaactgattg aaccagcgaa ataagccgcg gttggaatga tgctcgccct
     2701 tggccaccgg cttgagcaac gtggcgcaca gcgccggcgt cacgacgata gccatcagca
     2761 ccgacagcac catcgctgca acgatcgtca acgagaactg gcggtagatg acgccgctgg
     2821 aaccattgag aaatgccatt ggaatgaaga ccgcggtgag caccgtggca atgccgatca
     2881 gcgcaccggt gatctgttgc atggatttgc gagtagcgtc ccgtggcgaa agcccttcgt
     2941 ccctcatcag ccgctcgacg ttttcaacca ctacgatggc gtcgtccacc aacaggccga
     3001 tcgcaagcac catcgcgaac atcgtcagca tgttgatcga aaatcccgcc gccttgagta
     3061 cggcgaacgt gcccagtagc accaccggca ctgcgatcgt cggaatcagc gtcgcgcgca
     3121 ggttttgcag gaacagccac atcaccagaa acacgagtac gatcgcttcg gtaagtgtct
     3181 tcaccacctc ggtgatcgat ttgcgcacga atggtgtcgt gtcataggcg acggcgtaac
     3241 gcagaccggc cggaaatccg ggctggaagc gttgcaacgc ttcttccacg gcggcggctg
     3301 tttttagcga attggctccg gaggtgagcg aaatgcccat gccggaggcg ggctggccgt
     3361 cgaaacgcgc ctcggcgcca tagctcgatg cgccgagctc aacgcgcgca acgtccccca
     3421 ggcgcaacgt cgagccgtcg gtattgctgc gcagaatgat gttgcgaaat tgctccggcg
     3481 ttgagagcct gcccaaggcc gtgacggtcg cattgagctg ctgccccggc acgtaaggca
     3541 gtccacccag ttgcccaaca gaaatctggg cgttttgcgc cttgatcgcc gtaacgatgt
     3601 cttcgggcat caacgagtaa gtggcgagct tgttcggatc aagccagatg cgcatcgcgt
     3661 acttgccgct gccgaagagc tgcacggtcc caaccccatc aatgcggctg agcggttcgg
     3721 caatattggt agcgaggtaa tcgttgacgt cggccgtagt cagggagggg ttgtcggaca
     3781 cgaagccaat tgccagcacg aaattggaat tggtcttggt gacggtgatg ccctgctgtt
     3841 gcacgatctg cggcagcaag ggcaccgccg attgcagctt gttctgcacc tgaacttgcg
     3901 caatatccgg gttggtgcca ctgccaaagg tcatcgtaat ggcgatgctg ccggcgctgt
     3961 cactgctgga cgtcatgtag agcaggccgt cgagaccaat catgttctgt tcgatgatct
     4021 gcgtgaccgc gtcctgcacg gtttgcgccg aggcgcccgg atagttggcg ttgaccacga
     4081 tcgagggcgg tgcgatctgt gggtagcgcg cgaccggcag gcgcacgatc gagacgatcc
     4141 ctgccatcat caggatgatg gcgatgaccc aggcaaagat cggacgatca ataaaaaagt
     4201 tcgacataga cgcccttagc gctgcgcggt agcgactgct acgggcgccg tctcacccga
     4261 tgctccagcc accgcctgtt caaccgtctg cacctgcata ccgggctgca ccttctgaac
     4321 gccctcaaca atcaggcgat caccggccga caggccgccc tccaccagcc attgatcacc
     4381 gatggtgcgc gccactctta cctcgcgcgc ttcgaccttg ttctgcgcat tgaccaccag
     4441 cgcggtcgca ttgcctcttg ggtctcgagc gatgccctgt tgcggtgcca acagaccatt
     4501 ggttacgacg ccttcacgga tgatcgcgcg cacgtacatg cccggcatca ggatgtgatc
     4561 tggattggga acgatcgctc gcagcagcaa ctcaccggta gtcgggtcga ccgtgacatc
     4621 ggcaaattgc agtttgcctt catgcccgta ttcggtgccg ttctccagca taatctttgc
     4681 tgccgctccc gcaccgccgc cctgcactcg gccggaatcg acctcctggc gcaattgcaa
     4741 ccattcgctg ctggattgat tcacatcaac atagatcgga tcgagctgtt gcaccgtggc
     4801 catggcatcg gtctgattct gcgtgaccag cgccccctga gtgacactcg acttaccgat
     4861 gcgaccggtg attggcgaga caatatgcgc gtaggcgaga ttgaccgcgg cgctatcgac
     4921 cgcggcctgt gccgctacga catcggcctg agcctgtcgc cacgcagcca ccgcgttgtc
     4981 attgtcctgc tgactaacgg catcgatctt gactagatcc gcgtcgcgtc tggccgtcgt
     5041 gcctgccgct tccactgtgg cctcggcctt ctgtagcgcg gccttggcat tgttgtactg
     5101 cgcgcggtac ggagcgtcat ccagttcgta aagcggttga cccgttttga cgaacgctcc
     5161 ttcggtgaag agccgctgct tgacgatacc gctcacctgc gggcgcactt ccgcaaccaa
     5221 gtacgccgcc gtgcgtcccg gtagttcccg agtcagcggc acgggctgcg gcttcaacgt
     5281 cacgatcgtg acctgctgag ccggcctggc gtattgcctc gcgctgttct gaccgcagcc
     5341 ggccaacagc agtccgaaag tcaaggccgc gacgacgaaa gaagggcgac cgctgacagt
     5401 cggtaaagca ggcatgcgac accttggagg ttggaactgc gttccagggg cctcggcaaa
     5461 atctacagtg gccgcgggct cccgggcagg agtgaatgaa cgttcattcg cattctaggg
     5521 tgcggcaaac cgggtggcaa tcccgcaacg cgcgaatagg agaaccaaga gacgctcggc
     5581 agcggagtct gcgacgaagc cgcaggactc caccgctgcc gtcggggggg caggatgtca
     5641 ttgctttaga acggttcgta gcccagcgac gcggcgcgca agtcaagcac ctcgccctgc
     5701 gagttgacgg tgcagatgac gcgcgcgacg gcgcgattgt cgtgcgcatc gcgcgcggtc
     5761 agctccagtt cgctgttgct ggcgagcgct gccaggttgc tggcgccggc ctcgtcgagg
     5821 tatcgcgtat tgagcagctt cagggtgttc ggcattttgg tggacagctc cgccatgaac
     5881 gccttgacgc agccgttgag cgcgcgcggc cgatccgtag cggcgccaat cgtcggcact
     5941 gcagcgataa cagcggcgat cgcggccatc ttgaatgttc gggtcatcat tttgtcagct
     6001 ccataatcag ggtgggggga atccccatgg attctgacaa tttcgacaat agttagttcg
     6061 acaaactatc cattttcatc catggatggc tgcccgcctc ggagcaagca cactgtgtcg
     6121 aaacgtatgc attgtttcgg ctgcaacatt ccgacgcccg acatccctga tctgatggcg
     6181 gactgcgcaa gccatcgtgg gtcaattcga agcggtcctt gtcatattcg gcgcggtcac
     6241 cacctgggcg gtcattccct ggggctggac gctgatgaaa ccgtgcttct gagtacgcgg
     6301 aaccggcttg cggcggctct tgcgccacag atcgtagatc gtgagcgccg ccgtcaggga
     6361 agccagcgtg acgaagtaga ccgaaggccc gaatgccgcc atcagacttc ccatcaacag
     6421 cggtccgacg gcggccgcgg caccattcag gagcagcaac gcgctgctgg cagccaccat
     6481 ctgcgctggc tccagtttgt cattcacgtg cgatatcgcc aatgaataga gtgtcagtac
     6541 cgagccgctg aataccgccg cgagcatcag aaacagcgcg cgcggcatgc cactgaagac
     6601 cgcaatgctc ccggcgacca gagttgcgat cgtgcacacg ccggcaatca cggtacggcg
     6661 gtcgatgcga tcggacaggc gacccaccgg atattgagta acgacagccg ccagaatgct
     6721 cacgctcatg aaggtcgcga cgccggcgac acccagacca ctcaggcgcg cgtagaccgg
     6781 ccccatcgag aacaggatcg ccgagatcag gccggatacc gtcaccgcta ccacaccgag
     6841 gggtgaattg cggtacaact cgcggtaccg cacggggcgc ggcagcgagc actcgggcat
     6901 tttctgtgca gataccacaa tcggcaccat cgccaacgaa atcagcgcag agatcaacat
     6961 gaatgggaac ggactgcgtg gattggcgag catcagcaga agctgcgcga cacccagccc
     7021 cacatacagc accgccatgt agaccgcgag cagccgcccg cgattcgcac tgcttgcgcg
     7081 gtcattcagc cagct
//
LOCUS       DATMRM010000335         2976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3585182, whole genome shotgun sequence.
ACCESSION   DATMRM010000335 DATMRM010000000
VERSION     DATMRM010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3585182"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VNZ06_09965"
     CDS             complement(<1..888)
                     /locus_tag="VNZ06_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWW21118.1"
                     /translation="MQTSAIVQDPETHERFCASQIHDPVACAEGSRVLMQLARRVAAS
                     ECTVLIAGESGTGKEVLARYIHAHSARARRVFVGLNCAAIPENMLEAVLFGWERGAFT
                     GAHNAHPGKFEQAQSGTLLLDEISEIPMTLQAKLLRVLQEREVERLGSRNSIALDVRV
                     IATTNRRLREEVAAARFREDLYYRLNVFPITLQPLRSRRDDILPLAMRLLAGHCRAGE
                     RIPAVHPSAAQLLLTYAWPGNVRELENVMQRALVLCDGDLIRDAHIVFDPAPSVAAVQ
                     APTPLPASAAAANDRSLLHL"
     gene            complement(1003..2214)
                     /locus_tag="VNZ06_09970"
     CDS             complement(1003..2214)
                     /locus_tag="VNZ06_09970"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWW21119.1"
                     /translation="MPERAVVTGPFVSSDARTVGPNSGAVGSLPTGSSPLIQKVNHLI
                     NQVAAFDSNVLVLGESGTGKEVVARAIHEASPRRLRPFVPINCGAIPAELLESELFGH
                     EKGAFTGALVTRKGRFEIADGGTIFLDEIGDMNPTMQVKLLRVLQERVYERVGSCVSQ
                     RCDVRIIAATHRDLEQAIAKGTFREDLFYRLNVVPIEMPALRERGEDLPHLIADLSER
                     IIGAGRPTVQFSAPAIEALRAYRWPGNVRELSNLIERMSIQCGNRAVTVADLPSRYRP
                     VDWDPASEVAAENLLATAPVAAVLAPALEALSRELAVGYPLTQESTEGEPPPMDAGLA
                     DVMLEEIPDGFDLRNYLEGLEQRLIVRALEEAGGTVAHAARLLGLRRTTLVEKLRKYS
                     LTSADGAVSET"
     gene            complement(2340..2900)
                     /locus_tag="VNZ06_09975"
     CDS             complement(2340..2900)
                     /locus_tag="VNZ06_09975"
                     /inference="COORDINATES: protein motif:HMM:NF028133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWW21120.1"
                     /translation="MFEDIDTVVLYDQLAYHDVLPVQWLQLPSPPDRFELIGIEEANL
                     LLLHACVAVEEHPVRDKNEDLGALAGEISRLDFKLNLVMQLLSKLIAREKMPPATSIN
                     FNGLGASWTCIGKPPPVGAHGLLRVQLRSSVPQPLEIPAEVAAVDGSTAKVKFPDISE
                     ALVELIQKLAFLRHRRQIADVRKARG"
BASE COUNT          547 a          965 c          923 g          541 t
ORIGIN      
        1 gaggtgcagc agcgaccggt cgttggcggc cgcggcgctc gcgggcaacg gggtcggcgc
       61 ctgaaccgca gcgacgctcg gagccggatc gaacacgatg tgcgcgtcgc gaatgagatc
      121 gccatcgcac aacaccaatg cgcgctgcat cacgttctcc agttcgcgta cgttgccggg
      181 ccaggcgtaa gtcaacagca gctgagctgc ggatggatgc acggccggta tgcgctcgcc
      241 ggcgcgacag tggcccgcca gcaaccgcat cgccagcggc aggatgtcat cgcgccgcga
      301 gcgcaatggc tgcagcgtga tcggaaacac gttcagacgg tagtacagat cctcgcggaa
      361 acgagccgca gcgacttcct cgcgcaagcg ccgattggtc gtggcgatca cacgcacatc
      421 cagcgctatc gaattgcggg aaccgaggcg ttcgacttcg cgctcctgca gcacccgcag
      481 cagcttggcc tgcaatgtca tcggaatctc ggagatttca tcgagcagca gcgtgccgct
      541 ctgcgcttgt tcgaacttgc cgggatgagc attgtgtgcg cctgtgaacg cgccacgttc
      601 ccagccgaac agcacggctt ccagcatatt ctcggggatc gccgcgcagt tgaggccgac
      661 gaacacgcga cgcgcgcggg ccgaatgcgc atggatgtag cgtgcgagca cttccttgcc
      721 ggtgccggat tcaccggcaa tcagtaccgt gcactcgctc gccgccacgc gacgcgccag
      781 ctgcatgagt actcgcgagc cttctgcgca ggccacggga tcgtggatct gactggcgca
      841 gaagcgctcg tgcgtttccg gatcctgcac tattgctgat gtctgcatgg gacagcccac
      901 cgcctcttca agtatgactt gtgaggacag aggagcaatt tgcgtgccgt cggaaaagtg
      961 tgaccggtct cacaccggcg gacgattgcc gcctgatcga cgtcaagttt ccgacacagc
     1021 tccatcggcg ctcgtgagcg agtatttgcg cagcttctcg accagcgtcg tacgacgcag
     1081 acccagtaaa cgcgcggcgt gcgccaccgt gccgccggct tcctccagcg cacgcacgat
     1141 cagacgctgc tccagcccct cgaggtagtt gcgcaggtca aagccatcgg gaatttcttc
     1201 gagcatcaca tcggccagtc ccgcatccat cggcggcggc tcgccttcgg tactttcctg
     1261 tgtgagcgga tagccgacgg caagctcgcg tgacagcgct tccagcgcgg gcgcgagcac
     1321 ggccgcgacg ggtgccgtcg ccagcaggtt ctccgcggcg acctcactgg caggatccca
     1381 gtccacaggc cggtagcgcg agggcagatc ggccaccgtc acagcgcggt tgccgcactg
     1441 gatcgacatg cgctcgatca ggttcgacag ttcgcgcacg ttgcccggcc atcggtaagc
     1501 cctgagcgcc tcgatcgccg gggcgctaaa ctgcaccgtg ggtcggccgg ccccgatgat
     1561 gcgctcggac aggtcggcga tcaggtgcgg caaatcttcg ccgcgctcgc gcagagccgg
     1621 catctcgatc ggaaccacat tcagtcggta gaacagatcc tcgcggaagg tgcccttggc
     1681 aatggcctgc tccaggtcac gatgggttgc cgcgatgatg cgcacatcgc agcgctgcga
     1741 gacacagctg ccgacacgct cgtagacacg ttcctgcagc acccgcagca gtttgacctg
     1801 catggtcgga ttcatgtcgc cgatctcatc gaggaagatc gtgccgccgt cggcgatctc
     1861 gaatcgaccc ttgcgcgtca ccagggcgcc ggtaaaggcg cctttttcgt ggccgaacag
     1921 ctcgctctcg agcagctcgg ccggaatcgc gccgcagttg atcggcacga acgggcgcag
     1981 gcgccgcggc gacgcctcat gaatcgcgcg cgcgaccact tccttgccgg ttccggactc
     2041 accgagcacc aacacattgc tgtcgaatgc ggcgacctgg ttgatcaggt gattgacttt
     2101 ctggatcagc ggcgatgacc cggtaggtag acttccgact gcacccgaat tcgggccgac
     2161 agtgcgggca tcgctgctga caaagggtcc ggtcacaacc gctctttcgg gcatgaagtc
     2221 atttttccgt cacctgaaat cgtgggcgac agactattgg acgacttatg gcaacagtga
     2281 ggtattccgt cacagccgcc ctgagcacgg tgtctcagtt tcggcgcaga cgacgccagt
     2341 cagccgcgcg ctttgcgcac gtcggcgatt tgccggcggt gacgcaggaa cgcaagcttt
     2401 tgaatcaact cgaccagagc ttcggaaata tcggggaatt taacctttgc agtactaccg
     2461 tcaactgcgg ccacttcggc cggaatctcc agcggctgtg gcactgagct gcgcagttgt
     2521 acgcgcagca agccatgcgc gcccaccggc ggtggcttgc cgatgcaagt ccaggacgca
     2581 cccagtccgt tgaagttgat gctggtcgcc ggaggcatct tctccctggc tatcagcttc
     2641 gagagcagct gcatcaccag gttgagtttg aagtccagcc gtgagatctc accggccagc
     2701 gcgcccaggt cctcgttctt gtcgcgcacc ggatgctctt ccaccgccac gcaggcatgc
     2761 agcagcagca gattggcctc ttcgatgccg atcagctcga agcgatcggg aggtgacggc
     2821 agctgcagcc actgcaccgg cagcacgtcg tgataggcca gttggtcgta cagcactacg
     2881 gtatcgatgt cctcgaacat accgctccat tgtactgctt atggggtcgc cggggctccc
     2941 gccgtcgcta cgctgaaact gcgcacctcc gggccg
//
LOCUS       DATMRM010000336         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2360174, whole genome shotgun sequence.
ACCESSION   DATMRM010000336 DATMRM010000000
VERSION     DATMRM010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2360174"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VNZ06_09980"
     CDS             complement(<1..1207)
                     /locus_tag="VNZ06_09980"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW21121.1"
                     /translation="MKPVAFAAFQQRGFRRFFVGNATAMMADNCEHVISYWVMYQKFH
                     SPELGGFAVISHWVPFLLFSGYSGALASRIDPRRMIQLGMLLFMSVSIAWGLLIATDT
                     LQVWHAAILLTVHGCAGVLWGPPSQLLIHDIVRHDQLPSAVRLTATARYVGMLLGPAV
                     GNGLMLLLGPAHGIFANALIYLPMLLWLWRAPYGPRYRQAAGAPPALQGWGDAFATLR
                     SIRGNRIILSMTMLAGAVSFFIGNSYQAQMPNFATDLGHGNAGVSYAALTAADAAGAL
                     GAAMMLEARGLLTPRVATAFALSFLWCCALGIFALTHSYALALWALFCAGFLELAFSA
                     MAQALVQLNAPAPLRGHVIGVYAMAASGLRFVSGFTVGILGGLIGVHRSLAGAAAALC
                     VVIALLFVWL"
     gene            complement(1204..>2128)
                     /gene="purD"
                     /locus_tag="VNZ06_09985"
     CDS             complement(1204..>2128)
                     /gene="purD"
                     /locus_tag="VNZ06_09985"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HWW21122.1"
                     /translation="HRTFTRANYDARWLRSQQAPIVIKASGLASGKGVIIAQSLQEAE
                     SAVQEMFAGRFGEAGSEIVIEEFMQGEEASFIVMADGAHVLPLASSQDHKRLLDNDEG
                     PNTGGMGAYSPAPVVTPAVHRRVMREIIGPTIRGLREDGSPFTGFLYVGLMVGSDGTV
                     RVVEFNCRLGDPETQPILARLRSDLTTLCEAAVEGRLQTIEAKWDSRHALGVVLAAAG
                     YPDKVRTGDVIQGLESAAGKPGKLFHAGTRRVDGRCITSGGRVLCAVGLGRRISDARR
                     NAYGLVNCISYDGMNYRRDIGHRAIARERDS"
BASE COUNT          423 a          700 c          668 g          337 t
ORIGIN      
        1 caagccagac aaacagcagc gcaatcacga cgcacagcgc tgccgcggca cctgccaacg
       61 atcggtgcac gccgatcagc ccgccgagaa ttcccacggt gaagccgctg acgaagcgca
      121 gccccgaagc ggccatggca tacaccccga tcacgtgacc gcgcagaggg gcgggcgcgt
      181 tcagctgaac cagcgcttgt gccatggcac tgaacgcaag ttccaggaag ccggcgcaga
      241 acaatgccca gagtgccagc gcataggagt gcgtgagcgc gaaaatgccc agcgcgcaac
      301 accacaggaa gctcagcgcg aacgcggtgg ctacgcgcgg tgtcaacaac ccacgcgcct
      361 cgagcatcat cgctgcaccc agcgcaccgg ccgcgtccgc cgccgtcaga gcggcatatg
      421 agacgccggc attgccatga cctaagtcag tggcaaagtt cggcatctgc gcttgatagg
      481 aattgccgat gaagaaactg accgcaccgg cgagcatcgt catcgacaga atgatgcggt
      541 tgccgcgaat gctgcgcaga gtggcgaatg cgtcgcccca gccctgcagc gccggaggcg
      601 caccggccgc ttgtcgataa cgtggtccgt atggcgcccg ccacagccac aatagcatcg
      661 gcagatagat cagcgcgttg gcaaagatgc cgtgcgccgg tcccagcagc agcatcagtc
      721 cgttgccgac cgcaggcccg agcagcatgc ccacatagcg cgcggttgcg gtgagccgta
      781 ccgcgctcgg cagctggtcg tgtctgacga tgtcgtggat caacagttgc gagggcgggc
      841 cccacaacac gccggcgcag ccgtggaccg tcagcagaat ggcagcgtgc cacacctgca
      901 gcgtgtcggt cgcgatgagc agaccccagg cgattgagac cgacatgaac agcagcatgc
      961 ccaactggat catgcgtcgc ggatcgatgc ggctggcgag cgcgcccgaa tagccggaaa
     1021 acagcaggaa cggcacccag tgcgagatca cggcgaagcc gcccagctcc ggggagtgga
     1081 acttctggta catcacccag tagctgatga cgtgttcgca gttgtcggcc atcatcgcgg
     1141 tagcgttgcc gacgaagaac ctgcggaagc cgcgttgttg aaacgcggcg aaagcgaccg
     1201 gtttcacgaa tcacgttcgc gtgcaatcgc gcgatggccg atgtcacgac gatagttcat
     1261 gccgtcgtac gagatgcagt tgaccagccc gtaggcattg cgccgcgcat cgctgatgcg
     1321 cctgcctaag cccacggcgc acagcacgcg tccaccgctg gtgatgcagc ggccatcgac
     1381 tcgtcgcgtg ccggcatgga acagcttgcc cggttttcct gccgctgatt ccaggccctg
     1441 aatcacatcg ccggtgcgaa ccttgtcggg atagcccgct gccgcgagga ccacgcccag
     1501 ggcgtgacgc gaatcccatt tggcctcgat ggtttggagc cggccctcga cggcggcctc
     1561 gcacagcgta gtcaaatcgc tgcgcaagcg cgcgaggatc ggttgagtct ccgggtcccc
     1621 gagtctgcag ttgaattcga cgaccctgac agtgccgtcc gaccccacca tcagtccgac
     1681 atacaaaaac ccggtaaacg ggctgccgtc ctcgcgcaga ccgcggatcg tcggtccaat
     1741 gatttcgcgc atgacgcgcc ggtgcaccgc gggggtgacg accggtgccg gcgagtaggc
     1801 gcccatgccg ccggtattgg gaccctcatc gttgtcgagc aggcgtttgt gatcctgcga
     1861 gctggccagt ggcaatacgt gcgcgccatc cgccatcacg atgaaactgg cctcctcgcc
     1921 ctgcatgaac tcttcgatca cgatttcgga gccggcttcc ccgaagcgac cggcgaacat
     1981 ctcctgcacg gcgctttcgg cttcctgcag gctttgcgcg atgatcacgc ccttgcccga
     2041 tgccaggccg ctggccttga tcacgatcgg cgcctgttga ctgcgcagcc agcgggcgtc
     2101 gtagttggct cgggtgaacg tacgatgg
//
LOCUS       DATMRM010000337         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1439303, whole genome shotgun sequence.
ACCESSION   DATMRM010000337 DATMRM010000000
VERSION     DATMRM010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439303"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VNZ06_09990"
     CDS             complement(<1..812)
                     /locus_tag="VNZ06_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004654475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWW21123.1"
                     /translation="MRFATVFDVIVVGGGHAGTEAALAAARMGRSTLLLTHSIETLGA
                     MSCNPAIGGIGKGHLVREIDALGGAMARAADRAGIQFRMLNASKGPAVRATRAQADRQ
                     LYKRAIRSVLENQPGLSLFQQEASDLRLHGEQVCGVVTASGIEFEARAVILTVGTFLA
                     GRIHVGLTNHPGGRAGEAPSNSLALRLREIAPRTARLKTGTPPRIDGRTIDYSTLAVQ
                     HSDQPLPVFSFLGTAAEHPRQVPCHITHTNERTHAIIRAAFDRSPMFTGAIEG"
     gene            complement(942..2333)
                     /gene="mnmE"
                     /locus_tag="VNZ06_09995"
     CDS             complement(942..2333)
                     /gene="mnmE"
                     /locus_tag="VNZ06_09995"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019580343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HWW21124.1"
                     /translation="MEHQPSHRGHGREALTDTIVAAATPPGRGGIAIVRISGPQTEGI
                     GRQLVRTLPGARSATSSAFYGADGLPIDQGLACYFPAPHSYTGESVLELHCHGSPVVV
                     EALIRRVLQLGARRAGPGEFTQRAYLNAKLDLAQAEAVADLIDAASEGAARAALRSLQ
                     GEFSRRVVALSERLNELRTYVEAAIDFAEERLDLLSDRALGDRLQGAIAELHALQLTG
                     RQGRLLTHGITVVVAGRPNAGKSTLLNRLAGHDAAIVASTPGTTRDVLRERITLDGLA
                     LTLLDTAGLRPASDEVEAEGIRRARAAMQGADRILFVIDAAADPEARGFLEERDSLPA
                     QVPVTVLLNKIDLTTPAPPASLSTQSTAPILALSALTGAGIDALVSHLKQTAGMDSGT
                     DALSARARHLEALSAVDGNLSAAARLLSGGRTPELVAEELKRAQGALGEIVGAESSDE
                     LLGRIFSSFCVGK"
     gene            complement(2288..>3308)
                     /gene="yidC"
                     /locus_tag="VNZ06_10000"
     CDS             complement(2288..>3308)
                     /gene="yidC"
                     /locus_tag="VNZ06_10000"
                     /inference="COORDINATES: protein motif:HMM:NF002352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HWW21125.1"
                     /translation="QILRYNTPVERSYFHPDTYAYKGPALFDGQKFAKLDLTKTPTLN
                     QNIKDGWLAATQHDFVAAVVPPSGTDYRYQLQTQGNEFLLKAQASLQQLEAGASSDTR
                     ETLFVGPKVQKLLDQVHPKLNLLADYGTLRIIATPLFWLLEHVHSLIGNWGWTIIIVT
                     GLIKLLFYPLAETSGKSMAKMKALTPRMNSLRETYKDDREKLNRAMVELYQREKVNPL
                     SGCLPMLIQIPVFLAFYWVLRDSVEMRQAPFMLWINDLSSRDPFFVLPAIMAGAMYLQ
                     FKLNPQVGDPMQQKMFAIMPFAMSATFAFFPAGLVLYYVTNTLLSILQQWNINRRIGA
                     TVAKR"
BASE COUNT          620 a         1056 c         1041 g          591 t
ORIGIN      
        1 ccctcgatcg caccggtgaa catcggcgag cggtcgaacg cggcacggat gatcgcgtgc
       61 gtgcgttcat tggtatgggt gatatggcat gggacctgac gcggatgttc ggcagcggtt
      121 ccgagaaacg agaacaccgg cagcggttgg tcgctgtgct gaaccgccag cgtcgaatag
      181 tcgatcgtcc ggccgtcaat gcgtggcggc gtaccggtct tcagacgcgc tgttcgcggt
      241 gcgatttcgc gaagccgcaa cgccagcgaa ttcgacggtg cctcaccggc ccggccgccc
      301 ggatgatttg tcagcccgac atgaatgcgc ccagccagaa aggtcccgac cgtcaggatg
      361 actgcccggg cttcgaactc gatcccgctg gcggtgacga cgccgcacac ctgctcgccg
      421 tgcaggcgca gatcggaggc ttcctgctgg aacagcgaca gtcccggctg gttctcgagc
      481 acggatcgaa tcgcacgctt gtaaagctgg cgatcggcct gagcgcgggt ggcccgtaca
      541 gccggaccct tgctggcatt cagcatgcga aactggatgc ctgcacggtc cgcggcgcgc
      601 gccatcgcgc cgccgagcgc gtcgatctcg cgcaccagat ggcccttgcc gatgccaccg
      661 atggccgggt tgcagctcat cgcgccgaga gtttcgatgc tgtgcgtgag caacagggtc
      721 gagcgaccca tgcgggcagc cgccagcgca gcctcggtac cggcgtggcc accgccaact
      781 acgatcacat cgaagactgt cgcgaaacgc atggggcgcg cagtgtgcaa tccgatctgg
      841 gccgacgcaa ctagaggtca ggtcggcgca attgagtcat aaacgatgcc ttctctgacg
      901 catcggcccg cctattgaac caattgagat acattagacg gctatttacc cacacagaag
      961 gacgagaaaa tccggcccag caactcatca gacgactccg caccgacgat ctcccccagg
     1021 gcgccttgtg cgcgcttcaa ttcttcggcg accagctccg gggttctccc gcctgacaac
     1081 aagcgcgccg cggcggacag gttgccatcg acggcgctga gcgcctcgag atggcgggcg
     1141 cgcgccgaga gcgcatccgt cccgctatcc atccccgcgg tttgcttcag gtgcgacacc
     1201 agcgcgtcga tccccgcgcc cgtgagtgca gagagcgcca ggatcggtgc ggtagactga
     1261 gtggacaggc tcgccggcgg cgctggcgtg gtgagatcga tcttgttgag cagtaccgtg
     1321 accggtacct gtgccggcaa tgaatcgcgc tcctcgagaa aaccgcgtgc ctcagggtcc
     1381 gccgccgcat cgatcacgaa cagaatccgg tccgcgccct gcatcgccgc ccgtgcacgc
     1441 cggatcccct cggcctcgac ctcgtcgctg gcgggtctca agcccgcggt gtcgagcagg
     1501 gtgagcgcca gcccgtcgag cgtgatgcgt tcacgcagca catcgcgcgt ggttcccggg
     1561 gtcgatgcca cgatcgccgc atcgtgaccg gcaagccggt tgagcaatgt cgatttgcca
     1621 gcattcggtc tgccagcgac gacgacggtg attccgtgcg tcagcagccg accctggcgc
     1681 ccggtaagct gcagcgcatg cagttcggcg atcgcgcctt gcaagcgatc ccccagcgct
     1741 cgatcggaca gcaggtcgag acgctcctcg gcgaaatcaa tcgctgcctc gacatacgtt
     1801 cgcagctcgt tcaagcgctc actcaaagcg acgacgcggc gtgagaactc tccctgcagc
     1861 gagcgcaagg ccgcgcgcgc ggcaccctcg gaggcggcat caatcaggtc ggccaccgct
     1921 tcggcctgcg ccagatcgag cttggcgttc agataggcgc gttgagtgaa ttcaccgggt
     1981 ccggcgcgcc gggcgccgag ctgcagtact cggcgtatca gtgcctcgac caccaccgga
     2041 ctgccgtgac agtgcagctc cagcacggat tcgccggtgt aggaatgtgg cgctggaaag
     2101 tagcaggcga ggccttggtc gatcggcagc ccgtcagcac cgtagaaggc gctgctggtc
     2161 gcgctgcgcg cgccgggcaa tgtacgaacc agttgccgcc cgatgccttc ggtctgcggc
     2221 ccggaaatgc gcacgatcgc aatcccacca cgccccggcg gcgtggctgc cgcgacgatc
     2281 gtatcggtta gcgcttcgcg accgtggccc cgatgcgacg gttgatgttc cactgctgca
     2341 gaatcgacag gagagtgttg gtcacgtagt agagcaccaa tccagcggga aagaacgcga
     2401 aagtggctga catcgcgaac ggcatgatcg cgaacatctt ctgctgcatc ggatcgccga
     2461 cctggggatt gagcttgaac tgcaggtaca tcgccccggc catgatggcc ggtaacacga
     2521 agaacggatc gcgtgacgac agatcattga tccacagcat gaacggcgcc tggcgcatct
     2581 cgacgctgtc gcgcagcacc cagtagaagg ccaggaatac cgggatctgt atcagcatcg
     2641 gcagacagcc cgacagtggg ttgaccttct cgcgttgata gagttcgacc atcgctcgat
     2701 tgagcttctc gcgatcatcc ttgtaagtct cgcgcagcga gttcatgcgc ggcgtcagtg
     2761 ccttcatctt cgccatcgat ttgccgctgg tctccgccag tgggtagaac aacagcttga
     2821 tgaggccggt cacgatgatg atcgtccagc cccagttgcc gatcagtgag tgcacgtgct
     2881 cgagcaacca gaacaacggc gtggcgatga tccgcagcgt gccgtaatcg gccaacagat
     2941 tgagcttcgg atggacctga tccagaagct tctgcacctt cggcccgacg aacagtgttt
     3001 cgcgcgtgtc gctgctggcg cccgcctcca actgttgcag cgatgcttgc gccttgagca
     3061 gaaactcatt gccctgagtc tgtaactggt agcgatagtc cgtgcccgat ggcggaacca
     3121 ccgcggcaac gaaatcatgc tgagtcgccg cgagccagcc atccttgatg ttctgattca
     3181 gcgtgggggt cttggtcaga tccagcttcg cgaacttctg tccgtcgaac aatgccggcc
     3241 ctttgtacgc ataggtatcg ggatgaaagt acgagcgctc gaccggcgtg ttgtatcgca
     3301 gaatctgc
//
LOCUS       DATMRM010000338         8582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1439547, whole genome shotgun sequence.
ACCESSION   DATMRM010000338 DATMRM010000000
VERSION     DATMRM010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8582
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439547"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2582)
                     /gene="gltB"
                     /locus_tag="VNZ06_10005"
     CDS             complement(<1..2582)
                     /gene="gltB"
                     /locus_tag="VNZ06_10005"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HWW21126.1"
                     /translation="MDNQSTRGLYRESFERDGCGFGLIASLDDKPSHWLVKTAIASLN
                     RLTHRGAIATDGKTGDGCGVMLKKPEKFLRALARDAKIKLSQQFASGLVFLNQDATLA
                     EAARTELKTQLEREALVVAGWRVVPTTPEACGPEALKTLPSIEQLFVNCRIADIDEAS
                     FNRKLFLARRRAEKALPADAMFYVPSLSASTIVYKGMVMPQHLVEFYPDLADPRLESS
                     VVVFHQRFSTNTLPQWRLAHPYRYISHNGEINTIEGNRQWALARACVLRSPVLPDLSD
                     IAPLVTLTGSDSQSLDNMLEVLLMGGLDVPHALRLLIPPAWQSVDTIDSDLRAFYEFY
                     SAHMEPWDGPAGVVMTDGRYAACTLDRNGLRPARFVITKNRHLTIASETGVWDYAPED
                     VVRKGKLGPGEIVALDLQTGALLETRDVDAMLKSRHPYKAWLKKGVRYLQSDLIDPRL
                     AAEPMDRETLAVYQKMFHITAEERDEIIRVLAEDESEAVGSMGDDTPMPVLSHKIRSL
                     CDYFRQQFAQVTNPPIDPLRESIVMSVGTQIGPECNVFTPSPEHARQIVLSSPILSQR
                     KLRQILASDDVAEAHEFIDLQYEPPEGLKAAIVRVCDQAEAAVRDGKLVLLLSDRYLV
                     QGKLPMHALLATGAVQHRLVETGLRCKCNLLVETGIARDAHHFACLIGYGATAVYPYM
                     AYQTLYEMMRRGQVKLGYAEQREIGRSYRRGIRKGLYKIMSKMGISTIGSYRAAQLFE
                     IVGLSSEVVDQCFRHTTSRIEGASFEDLEGDTRYLAMRAWNPRVGVEQGGIYKYVHGG
                     EYHMYNPDVVGALQAAVVSGDYEHYRLFSQLVNNRPVSTIRDLLGIKQAAKPIPIDEV
                     ESIEA"
     gene            2954..3505
                     /locus_tag="VNZ06_10010"
     CDS             2954..3505
                     /locus_tag="VNZ06_10010"
                     /inference="COORDINATES: protein motif:HMM:NF018813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HWW21127.1"
                     /translation="MALNATLHSLEIQLSDSDRGVYQALAFRVAQHPSETRDYLVTRV
                     LAYCLEYTQGLAFSRGGLAEPDEPALTIRDLSGTLTNWIEVGLPEAARLHKAAKSAAR
                     VVVYPHRDPGAWLARIRSEAIHRMDRIEIYAIDRELIAALVARLSRRMRFDCVSSERH
                     LYLSFDDGTIDGSIARLHLSDAV"
     gene            complement(3740..4855)
                     /locus_tag="VNZ06_10015"
     CDS             complement(3740..4855)
                     /locus_tag="VNZ06_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21128.1"
                     /translation="MFNKTTQAQGADNFPDAQVTAWRESDGTVNLSIPNIDTWRMRGS
                     DLEHLTVDASEIYSSTNTASQITEDLHNYWHWMMGPYSLDGKTFYSLAHSEWYGCLLV
                     GNCNALPSSRARTNSWANTIDSLKSDDGGATWQLNSVNGNHTVADAAYTWTGSQALAS
                     QIYLHALDHTGIFGPSRVVKQAGYYYSIAYYVHRNFSAIDPGAGVFEAPVDKSGFVIL
                     RTSDITNPNGWSAWTGGSGYGPVANGTFAVFAPKIAGVTLNAASVQLIYDTVAQCFIL
                     VHTLNQDGSPVYFSTTKSLANPAWSDAQEIVGTATLTTDPTGPVIGFGELFYPSILDD
                     SSAGYNYEFTSSGRPQLFYSTYPSRNIYRVPLTITYK"
     gene            4881..5087
                     /locus_tag="VNZ06_10020"
     CDS             4881..5087
                     /locus_tag="VNZ06_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21129.1"
                     /translation="MLTMVLGVDAPLEELELAPELELELVARAPLELPPEGVPLDELP
                     PCDPLLDEPPAPELELPVPDTLAS"
     gene            5397..5600
                     /locus_tag="VNZ06_10025"
     CDS             5397..5600
                     /locus_tag="VNZ06_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21130.1"
                     /translation="MTVVLAEVLLLGGVPAGGVLGGADVAPLEGGTLLEFELAVSLLE
                     PLQAASVVNAAMSAARLQSKRME"
     gene            complement(5830..7254)
                     /locus_tag="VNZ06_10030"
     CDS             complement(5830..7254)
                     /locus_tag="VNZ06_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWW21131.1"
                     /translation="MRPARWTLRGTWSAAALTVTLALAIPASAEDPFGTMQTLQRRTP
                     GMADPIGHDCLLPGKALTFAAAVDLALCRNPQTRAAWAAAHEQAAALGAAESAWLPQI
                     VGQAEGSRDIGQHVDVTGATVGSSQNTLDAALNLTWTLYDFGARGGRIRSAHRLLDAA
                     AETLSSVNQQVVLSVVQSYYGVVAADELLVAARTTESVTAHSLEIARSLRTGGVGTLG
                     DVLQAETANDQAIITRVQAEATAKTARGTLAVTLGLQADHLLTLDPQSVPAEVPALNA
                     KIADLMTEATRQRPDLAAAVAQRDAAEANVSVARAAGLPTISVVAGKDFITTSSVPNQ
                     NFTQIGVNISVPIFTGFSATYGVRQAKAAVEASEVNAEQVRLGVTLGVWNAYYALDSA
                     GQQLTATGSLTRTAESNEEVSLGRYQSGVATIVDVLTAQTAAANARVTRINAELGWQV
                     ARAELALALGRLSGAEPLEKLTLP"
     gene            complement(7257..>8582)
                     /locus_tag="VNZ06_10035"
     CDS             complement(7257..>8582)
                     /locus_tag="VNZ06_10035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HWW21132.1"
                     /translation="EVVGAFESETAAVFLRTAPAHEHLILYALVAFLVVMITLASVVK
                     LDRVVTGGGVVVPTAGAMYVSPFDTGIVKQVNVRAGDVVKKGQVLATLDPTFAHADLQ
                     QLQQRTSSDEAQVARLEAEIAGSPYVFSNTNRDQMLQGGIWQKRQAEYHYNLADFDGR
                     IHGAEAQVAQYVADSQQYSKRLKLANEVEKTYQPLLEKGYVSKLQVVQAADERTEMTR
                     LLSDAQNQMISLRQTLASLKAQREAYVQKWNSDAGSELVTVRNDLDLTRQSLEKAQKM
                     SDLASLNAPSDAVVLKVGKVSSGSVTAGGGVQAINQDPLFTLVPLDAPLEADINVPAG
                     DIGFIKVGDPVRLKLDAYRFLQHGTAKGAIQTISEGSFTTDENNVPVPPYFKVAVAIK
                     DIHLKNVPSGFRLIPGMTLAGDILVGRRTILSYLVEGGMRTGSEAMREP"
BASE COUNT         1612 a         2705 c         2558 g         1707 t
ORIGIN      
        1 gcctcgatcg attccacctc atcgatggga atcggcttcg cggcttgctt gatcccaagc
       61 aggtcgcgaa tggtggacac cggtcggttg ttcacgagct gggaaaacag ccgatagtgc
      121 tcatagtcgc ccgagaccac cgctgcctga agagcgccga cgacgtcagg gttgtacatg
      181 tggtactccc cgccgtgcac atacttgtag atgccgccct gctcgactcc gacccgcgga
      241 ttccaggcgc gcatcgccag gtagcgcgta tcgccttcca ggtcctcgaa cgacgcccct
      301 tcgatgcggc tggtcgtgtg gcggaagcac tgatccacca cttcgctcga cagacccacg
      361 atctcgaaca actgggccgc gcgatagctg ccaatggtcg agatgcccat cttcgacatg
      421 atcttgtaca ggcccttgcg gatgccgcgc cgatagctgc ggccgatttc gcgctgttcc
      481 gcgtagccga gcttcacctg gccccggcgc atcatttcgt acagcgtctg gtaggccatg
      541 tacggataga ccgcggtcgc gccatagccg atcaaacagg cgaaatgatg ggcatcgcgc
      601 gcaataccgg tctccaccag tagattgcat ttgcagcgca gtccggtctc gaccagacga
      661 tgctgaaccg ctccagttgc cagcagcgcg tgcatcggca gcttgccctg caccaggtag
      721 cggtcggaga gcagcaggac gagcttgccg tcgcgcaccg cggcttccgc ctgatcgcat
      781 acccgcacaa tggcggcctt cagtccttcc gggggctcgt actgcagatc aataaactca
      841 tgggcctcgg ccacgtcatc cgaggccagg atctgacgca gcttgcgctg cgagagaatc
      901 ggggaactga gcacgatctg gcgtgcatgt tccggactgg gggtgaatac attgcattcc
      961 ggaccgatct gcgtgccgac cgacatgacg atcgattcgc gcagtggatc gatcggcgga
     1021 ttggtcactt gcgcaaactg ctgccggaag taatcgcaca gcgaacggat cttgtgtgac
     1081 agcaccggca tcggtgtgtc atcccccatt gacccgaccg cttcgctctc atcctccgcc
     1141 agcacgcgga tgatctcgtc gcgctcttcg gcggtgatgt gaaacatctt ctgatagacc
     1201 gcaagcgtct cgcggtccat cggctcggcg gcgagccgcg gatcgatcag gtcggactgc
     1261 aggtagcgca cacccttctt gagccatgcc ttgtaggggt gacgagattt cagcatcgca
     1321 tcgacgtcgc gggtctcgag cagcgcgccg gtctgcaggt ccagcgctac gatctcgccg
     1381 ggtcccaatt tgcccttgcg taccacgtct tcgggcgcgt agtcccaaac cccggtctcc
     1441 gatgcgatcg tcagatggcg gttcttggtg atcacgaagc gcgccggccg caggccgttg
     1501 cgatccagcg tgcaggcggc atagcgcccg tcagtcatca ccacacctgc cggtccatcc
     1561 cacggttcca tatgggcgga atagaactcg tagaacgcgc gcaggtcgga gtcgatcgta
     1621 tccactgact gccaggccgg aggaatgagc agccgcagtg cgtgcggtac gtccagaccc
     1681 cccatgagca atacctcgag catattgtcg agactctgag agtccgaccc cgtcaaggtc
     1741 accagggggg cgatgtcgct caagtctggc agaaccgggg agcgcagcac gcaggcccgc
     1801 gccagcgccc actggcggtt accctcgatg gtattgatct caccgttgtg cgagatgtag
     1861 cgataggggt gcgccagacg ccactgcggc agcgtgttgg tggaaaagcg ctggtgaaac
     1921 accacgaccg aggactcgag ccgaggatcg gccaggtccg ggtagaactc gaccagatgc
     1981 tgcggcatga ccatgccctt gtagactatg gtgctggccg acaggctggg cacatagaac
     2041 atcgcatcgg ccggcagcgc cttttcggcc cggcgacggg ccagaaacag tttgcgattg
     2101 aagctggcct cgtcgatgtc cgcgatgcgg cagttgacga acagctgttc gatgctgggc
     2161 agcgtcttca gggcttcggg tccgcaggcc tcgggcgtcg tgggcacgac ccgccacccg
     2221 gcgacgacga gtgcctcgcg ctcgagttgg gtcttcagtt ccgtccgcgc cgcctccgcc
     2281 agagtggcgt cctggttcag gaacaccagg ccggatgcga actgctggct gagcttgatc
     2341 ttggcgtcgc gcgcgagcgc acgcaggaat ttctcgggct ttttgagcat caccccgcag
     2401 ccatccccgg tcttgccatc cgtcgcgatc gcgccgcggt gggtcaggcg gttcagggag
     2461 gcaatggcgg tcttgaccag ccaatggctc ggcttgtcgt ccaggctggc gatcagcccg
     2521 aagccacatc cgtctcgctc aaacgattcg cggtaaagcc cgcgggtgct ctgattgtcc
     2581 ataggtcgcc cgtccctcgg tttaaccctt atcaggcgcg tcggtcgtgt ggccgcttag
     2641 gcgagggacc cgcgcggcga tcgagcagaa agcttgcttg cgatttccgg gactgaagag
     2701 gcgtcagcac cagctgccca tccaatatac cgatcgggga catgcggtca aattcattca
     2761 gtgttgattc atatgcgcag ccgtaatgtg tgtcccaggc gccaacggtg gacctcgaag
     2821 ctcccagcag cccttaacgc gcagctgggt ttgagccctc cctccgccgt gacgcctgaa
     2881 ccaacccaac ccggcgggct cgtgatgcga attgcgcgaa cccgccgggc cgcgttttag
     2941 ctactgttgc cgcgtggcac tcaacgcaac cctccacagc ttggagatcc agctcagcga
     3001 ctcggaccgg ggtgtctacc aagccctggc cttccgggtc gcgcagcacc cgtccgaaac
     3061 ccgcgactac ctggtgaccc gggtactggc ctactgtctg gagtacacgc agggactcgc
     3121 cttctcccgg ggcgggctgg ctgagcccga tgagcccgcg ctgacgatcc gggatctgag
     3181 cggcaccctg acgaactgga tcgaggttgg attgccggaa gcggcccgat tgcacaaggc
     3241 ggcgaagagc gcagctcgcg tcgtggtcta cccgcaccgg gatccgggcg catggcttgc
     3301 gagaatcagg tccgaagcga tccaccgcat ggatagaatc gagatttatg caatcgatcg
     3361 tgaattaatc gcggcgctgg tcgctcgact cagccgccgt atgcgattcg actgtgtctc
     3421 tagcgagcga catctctatc tttcgtttga tgacggtacg atagacggca gcatcgcgcg
     3481 cctccacctc agcgatgcgg tgtgaacgcg cacgatgaaa tgagaaacaa ctctcggtac
     3541 cgcacgaggc actaagagat atttaatccc gcttgagttc gaacgtgcga acgcaggcaa
     3601 gcggctccga gactcccagc agcccttttc ctgctcggat ttgagtctcg cccgctcgat
     3661 acgcaagacc tcgatccggc gggcccctcg aacctttgat cgtccggccc gccggccatc
     3721 cgcgcaagcc cgccagtcct tacttataag taatcgtcag ggggacgcgg taaatatttc
     3781 ggctcggata ggtactgtag aacagctgcg gcctaccgct gctggtgaac tcatagttgt
     3841 agcctgcgga gctgtcgtcc aaaatcgaag ggtagaacaa ctcgccaaat ccgatgacgg
     3901 gaccggtcgg atccgttgtc agggtggcag tgccgacgat ctcctgagca tcgctccacg
     3961 caggatttgc aaggctcttg gtggttgaga agtagaccgg gctaccgtcc tgattgagcg
     4021 tatgaaccag gatgaaacac tgggccacgg tgtcgtagat gagctgcacc gatgctgcat
     4081 tcaacgtcac accggcgatt tttggtgcga acaccgcgaa agttccattg gccacgggcc
     4141 catagccact acccccggtc caggccgacc atccattcgg gtttgtaatg tctgatgtgc
     4201 gcagtatcac gaagccagat ttgtcgacgg gcgcctcaaa cacgccggct cccgggtcga
     4261 ttgcactgaa gtttcggtga acgtagtaag cgatactgta gtagtagcca gcttgcttca
     4321 ctacacgact aggtccgaag atgcccgtgt ggtcgagcgc gtgcagatag atctgactgg
     4381 caagcgcctg cgagcccgtc cacgtatacg ccgcatccgc aaccgtgtga ttgccattga
     4441 cactgttcag ctgccaggtc gcacccccat cgtcagattt caacgaatca atcgtgtttg
     4501 cccaactatt cgtccgcgcg cgcgaactcg gcaacgcatt gcagttacct acgagcagac
     4561 acccatacca ctcgctgtgc gcgagggagt agaacgtttt cccgtccaga ctgtatgggc
     4621 ccatcatcca gtgccagtaa ttgtgcagat cctcggtgat ttggctggct gtattggtgc
     4681 tgctatagat ctcgctcgcg tcgacggtca ggtgctcaag atcactaccg cgcattctcc
     4741 aggtgtcgat gttcgggatc gacagattga cggtaccgtc cgattccctc cacgccgtca
     4801 cctgcgcatc cgggaagttg tccgcgcctt gcgcctgcgt ggtcttgttg aacacttcaa
     4861 cgggagcacc cgcgagctgg atgctgacga tggtcttggg cgtggacgca ccgctggaag
     4921 aactcgagtt ggcaccagag cttgagctgg aactcgtcgc tagggcgccg ctcgaactac
     4981 cgcctgaggg tgtaccactg gacgaactgc cgccgtgtga cccactgctc gacgaacctc
     5041 cggcgccgga acttgaacta ccggtccccg atacactcgc ctcctgagca atactgatcg
     5101 tcgccttgga taccgctccc aggctcgcgc cgtcggtact cgacatcaga cggacgctga
     5161 actgtttacc gatgccagcc gaggtgattg gcaccaaaat cgtcctcgtc gatacgtcgc
     5221 cgatgagcca gctcagagtg ccgttctgtg cgacgtaatc ggtgcccgaa gtggcagagc
     5281 cgttggcggt cgcatagttg accgagatcg actgagtggg cgagccgctg aggacgacat
     5341 caatacgcgc cgtaccggcg ctgacagcca ccgtataggc ggtcgctgag agctggatga
     5401 cggtcgtatt ggccgaggtg ctgctgctgg gaggtgtccc cgccggcggt gtgctcggtg
     5461 gcgccgatgt cgctccgctg gaaggcggaa cgctattgga gttcgagctg gcggtgtcgt
     5521 tgctcgaacc attgcaggca gcaagtgtcg tcaacgccgc gatgagcgct gcacggctcc
     5581 aatcaaaacg catggaatga gaccccgaat aaatcccagt caacgccagc atcctagcga
     5641 cgagatgcga cgcgacgaat gcgtagtgcc ggtgcgacac gtcgccaatc gcagaccaag
     5701 cagctcgcgt tcgcgatgtg cgagccacta cgcacggagc tgtggtccac tcctcaaagc
     5761 agtagcaatt ggttttactt ttgtcggtcc gtgcggaggg cccgccgatt tgccatattt
     5821 ggcaaagcgc tacggaagtg tcagtttctc cagcggctcg gcgcccgaaa gacggccgag
     5881 cgcaagagca agctccgccc gggccacctg ccagccgagc tccgcattga tgcgcgtcac
     5941 gcgcgcgttc gcggccgccg tctgcgcggt cagaacatca acgatcgtcg cgacgcctga
     6001 ctgatagcga ccgagtgaaa cctcctcgtt gctctctgct gtcctggtca gagaaccggt
     6061 cgctgtcagc tgctggcctg ccgaatccaa agcgtagtac gcgttccaca cgccgagtgt
     6121 cacacccagg cgcacttgct cggcattgac ttcactcgcc tcgaccgcag cctttgcctg
     6181 acgcacgccg taagtcgcgc tgaatcccgt gaagatcggt acgctgatgt tcacgccaat
     6241 ctgggtgaag ttttgattcg gcacgctgct ggtagtaatg aaatctttgc cagccaccac
     6301 cgatatcgtt ggcagcccgg cggcacgcgc gaccgacacg ttggcttccg cggcgtcacg
     6361 ctgcgcgacg gcagcggcca ggtcggggcg ctgccgcgtg gcttccgtca tcagatccgc
     6421 gatcttggcg ttcaatgcgg gcacttcggc cggcacagac tgcggatcga gggtcaggag
     6481 atgatcggcc tgcaggccga gtgtcacggc cagagtgccg cgcgctgtct tcgccgttgc
     6541 ctcggcctgc acgcgcgtga tgatggcctg atcgttcgcg gtctccgcct gcaatacgtc
     6601 gcccagagtt ccgacgccac cggtgcgcaa cgagcgtgca atctccaggc tgtgcgccgt
     6661 caccgattca gtggttcggg cggccacgag cagctcatcg gcagcgacca ctccataata
     6721 gctttgcacc acactcagca cgacctgctg attgacgcta ctgagtgtct cggccgcggc
     6781 atccagcaat cggtgcgcac tgcgaatgcg cccgccgcgc gcaccgaaat catagagcgt
     6841 ccaggtcaga ttaagcgctg cgtcgagcgt attttggcta gagcccaccg tggcacccgt
     6901 cacatcgacg tgctgcccga tatcgcggct accctccgct tgtccgacga tctgcgggag
     6961 ccaggcgctc tcggccgcgc cgagcgcagc cgcctgttcg tgtgctgccg cccaggccgc
     7021 tcgcgtttgt gggttgcggc acagagcaag atcgactgca gccgcaaagg tcagcgcttt
     7081 accgggcaat agacaatcgt ggccgatagg atccgccatt cccggagtcc gccgctgcag
     7141 tgtctgcata gttccaaacg gatcctcggc cgaggccggg atcgccagtg ccaatgtgac
     7201 ggtgagcgcc gcggccgacc acgtgccgcg cagagtccat cgtgcgggcc gcatctctag
     7261 ggttcccgca tcgcttccga accggtgcgc ataccacctt cgaccagata cgacagaatc
     7321 gtgcgccgtc ccacgagaat atcccctgcc aatgtcattc caggaatcaa tcgaaatccc
     7381 gacggaacgt tcttgagatg aatgtctttg atcgcgactg ccactttgaa gtatggcggc
     7441 accggcacgt tgttttcatc cgtcgtgaag gagccttcgc tgatggtttg gatcgcacct
     7501 ttggccgtcc catgctgcag gaatcggtag gcgtcgagct tgagccgaac gggatcaccc
     7561 accttgatga aaccgatgtc ccccgcgggc acgttgatgt ccgcttccag cggcgcgtcg
     7621 agcggtacca gcgtgaacag cggatcctga ttgattgcct ggacaccgcc accggccgtc
     7681 accgatcctg aggaaacctt gccgactttc agcaccactg cgtccgaggg cgcatttagg
     7741 cttgccagat cgctcatttt ttgcgccttt tccagacttt gacgagtcag gtccagatca
     7801 ttgcgaactg tcacaagttc cgagcccgcg tccgaattcc acttctggac gtacgcctcg
     7861 cgctgcgctt tgagcgatgc cagcgtttga cgcagggaaa tcatctgatt ctgagcatcc
     7921 gacagcaacc gcgtcatctc agtgcgttca tcggcagcct gcaccacctg aagtttcgag
     7981 acgtagccct tttcgagcag cggctgatac gtcttctcca cctcattcgc cagtttcagt
     8041 cgtttgctgt actgctggga atccgccacg tactgcgcga cctgtgcttc agcgccgtga
     8101 atgcggccgt cgaagtcagc cagattgtag tgatactcgg cctgccgctt ctgccagatc
     8161 cccccttgca gcatttgatc ccggtttgtg ttcgaaaaca cgtaggggct gcctgcgatt
     8221 tctgcttcga gccgcgcgac ctgcgcttca tcgctcgagg tccgctgctg cagttgttgc
     8281 agatccgcat gggcaaacgt cgggtcgagt gtcgccagca cctggccctt ttttacgacg
     8341 tcgcccgctc tgacgttcac ctgcttgacg atccctgtgt caaacggcga gacatacatg
     8401 gcgccggcgg tcggtacgac gacccctccg cccgtcacta cccggtcgag cttgacgaca
     8461 gacgccagcg taatcatcac aaccagaaat gccaccagcg cgtacaggat cagatgttca
     8521 tgtgcaggtg cggtgcgcag aaagaccgcc gcggtttcgg actcgaacgc gccaacgact
     8581 tc
//
LOCUS       DATMRM010000339         4560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4552956, whole genome shotgun sequence.
ACCESSION   DATMRM010000339 DATMRM010000000
VERSION     DATMRM010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4552956"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VNZ06_10040"
     CDS             <1..891
                     /locus_tag="VNZ06_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21133.1"
                     /translation="IALIIPALGWLVALLGALFSIYLLNMGLPFTMKCPPEKSVGYTA
                     VTIIVMIVIYLLIAFVVGRVSLMSGMNPYGAAFSSSLSERPTRGNFAPGSPGAALQSY
                     ADSLQKASKQMDTAQKSGDANAQGAALGAIVGAALGGNTKVESLAPDRIKPFIPDSLA
                     GLPRTSISVSRNGALGMQMSTGTATYSDGGQHSLHLEIADTGSLKGLVGFATGYAGTE
                     QDTETDTGYEKIYKSGGQLVHEKWDNRGSYGEYGVVVGDRFSVKVSGNASNIGELKSA
                     VNALDLSGLAALKGEGVQSN"
     gene            948..1691
                     /locus_tag="VNZ06_10045"
     CDS             948..1691
                     /locus_tag="VNZ06_10045"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HWW21134.1"
                     /translation="MNRLEGRFALITGATRGIGRAIAKSLAAEGATIAVNYQSSEADA
                     RSLVDELDKAGRKTLLFKCNVGERASWTTMLEKVKDNWGRLDILVNNAGITRDKSMRK
                     MTDDDWNAVLQINLNACYYGVSAFMPMMIDQKFGRIVNISSFVGQAGNFGQANYAASK
                     GAIIAFTKTAAIEMAKYNVTCNSLAPGFTDTSMLAKVPENVQQQILARIPMGRFGRPE
                     EIAKAVLFLAADGDYITGQQINVNGGVYM"
     gene            complement(1710..2663)
                     /gene="phaC"
                     /locus_tag="VNZ06_10050"
     CDS             complement(1710..2663)
                     /gene="phaC"
                     /locus_tag="VNZ06_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HWW21135.1"
                     /translation="MTGCSHKDVVWQSGKLTLYRYQPITRPLGAAPLLIVYALVNRPY
                     MMDLQEDRSLIRGLLASGRDVYLIDWGYPDGADRFTTLADYIDRLLAECVEHVLNAHQ
                     ISALNLLGVCQGGVLGLCYGALYPQRVSNLITMVTPVDFHTPADLLSKWVRGIDIAAF
                     VGEGNVSGEILNQLFLALMPFRLTQHKYVEMLSQASDPTQLKNFLRVEQWIFDSPDQA
                     GAAFREFVRCLYQENRLINHRLEIGGRRIDLRALQMPILNLIGKRDHLVPPASSRALG
                     ALVGSQDYTALELDLGHIGMYISARAQREVPAAIVDWLAAR"
     gene            complement(2660..3412)
                     /locus_tag="VNZ06_10055"
     CDS             complement(2660..3412)
                     /locus_tag="VNZ06_10055"
                     /inference="COORDINATES: protein motif:HMM:NF021246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HWW21136.1"
                     /translation="MSADPWGGWQAFSDAARSYMDSLSKASAQGASAQFGDFLRDQFA
                     SYSQPWGSQPWGSQPWGSAFAPAAASSPSSPLSPDVPAFGATREQQQRAQRMAQAAAR
                     MEAAQHRLQRLWSDVLREAASRFVSQLSASPPAAVTPESLRRLYDTWIDCAEDAYARV
                     AHGEAFCQAQAEFANAASQWRLEQQANIEHWSKFLDLPTRSEINSLTRRLRATEEQLR
                     SMSGSGGATRAPPRNSPSKPPTAKAKAKKKRT"
     gene            3611..4105
                     /gene="phaR"
                     /locus_tag="VNZ06_10060"
     CDS             3611..4105
                     /gene="phaR"
                     /locus_tag="VNZ06_10060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HWW21137.1"
                     /translation="MPSERLIRKYSNRRLYDTGASRHVTLEDLRQLIISGEKIKVVDD
                     KTGEDLTRAVLLQIISEQEQFGSPVLSAELLEMIIRFYGRPMQAMLSRYLEQSFSMML
                     RQQEVVQAEMTKALQSTPFAPLTELARKNMELWTEMQRATRDAFSGRTTAGSNPDDQD
                     PEGK"
     gene            4115..>4560
                     /locus_tag="VNZ06_10065"
     CDS             4115..>4560
                     /locus_tag="VNZ06_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWW21138.1"
                     /translation="MDVRKKTIVVTGAGRGIGRAIALQLAAHGANIAIFDLNSDDIEQ
                     TRSLCAAKAVDARGYHVNVADENEVTAAMATVVAEFGRLDGLVNNAGIVRDALLIKVK
                     DGAVVARMTLAQWNAVIGVNLSGVFLCAREAAAQMIQLGNGGVIVN"
BASE COUNT          951 a         1402 c         1401 g          806 t
ORIGIN      
        1 atcgcgctga tcattccggc gctgggctgg ctggtcgcgc tgctcggcgc actgttcagc
       61 atctacctgc tgaacatggg cctgccgttc acgatgaagt gcccgccgga gaagtcggtc
      121 ggctataccg ccgtgacaat catcgtgatg atcgtcattt acctcctcat cgctttcgtc
      181 gtcggccgcg tcagtctcat gagcggcatg aatccgtacg gtgccgcctt cagctcgagc
      241 ctgagcgagc ggccaacccg cggcaacttc gcaccgggca gcccgggcgc tgcacttcag
      301 tcctacgccg acagcctgca gaaagcgtcc aagcaaatgg atacggcgca gaaatccggc
      361 gatgcgaatg cgcagggcgc ggcgctcggt gccatagtcg gggcggcgct cggtggcaac
      421 accaaagtcg aatcgctggc gccggatcgc atcaagcctt tcatcccgga cagcctggct
      481 ggtctgccgc gcacatcgat ttccgtgtca cgaaacggtg cgctcggcat gcagatgtcc
      541 accggcaccg ccacctattc ggacggcgga cagcactcgt tgcatctgga gatcgcggac
      601 accggctcgc tcaagggact ggtcggattc gcgaccggct atgcgggcac ggaacaggac
      661 acggagaccg acaccgggta tgaaaaaatt tacaaaagcg gtggacagct ggtgcacgag
      721 aagtgggaca accgcggctc ctacggcgag tatggcgtgg tcgttggcga ccgtttctcg
      781 gtcaaggttt ctggcaacgc atcgaatatc ggcgaactga agtcggcagt gaacgctctg
      841 gacctctcgg ggcttgcggc actcaaaggc gaaggcgtac aaagcaacta agcattcatt
      901 cttcgccgtt acactcgtcg ctggcctggc aaacgggagt actcaccatg aacaggctcg
      961 aaggcagatt tgctctgatt accggcgcaa cacgtggcat cggcagagcg atcgccaaat
     1021 cgctcgccgc agaaggcgct acgatcgcgg tcaactatca gtcaagcgaa gccgatgccc
     1081 gctcgctggt ggacgaactc gacaaggcgg gacgcaagac gctgctgttc aagtgcaacg
     1141 tcggcgagcg agcgtcctgg acgacgatgc tggagaaggt caaggacaac tgggggcgac
     1201 tcgatattct ggtcaacaac gccggtatta cgcgcgacaa gtcgatgcgc aagatgaccg
     1261 atgatgactg gaacgcggta ttgcagatca atctcaacgc ctgctactac ggggtgtccg
     1321 ccttcatgcc gatgatgatc gaccagaaat tcggtcgtat cgtcaacatc agctcgttcg
     1381 ttggccaggc aggcaatttc gggcaggcca actacgcggc cagcaagggc gcgatcatcg
     1441 ccttcaccaa gacggcggcg atcgagatgg ccaagtacaa cgtcacctgc aattcgctgg
     1501 cgcccggttt caccgatacc agcatgctgg cgaaagtgcc ggagaacgtg cagcagcaga
     1561 tcctcgcccg cataccgatg ggacgtttcg gacgacccga agagatcgca aaggcggtgc
     1621 tgttccttgc cgcagacggc gactacatca ccggtcagca gatcaacgtc aacggcggcg
     1681 tctatatgta gtggtggcgg tgcttgcgcc tagcgtgccg cgagccagtc cacgatcgcg
     1741 gcgggcactt cacgttgcgc gcgcgcgctg atgtacatgc cgatgtgtcc gagatccagc
     1801 tcgagcgcgg tgtagtcctg actgccgacg agcgcaccca gtgcgcgcga agaggcggga
     1861 ggcaccagat gatcgcgttt gccgatcagg ttcaatatcg gcatttgcag cgcgcgcaga
     1921 tcgatccgcc gaccgccgat ctccagccgg tggttgatca gccgattttc ctgatagagg
     1981 cacctcacga actcgcgaaa cgcagcgccg gcctgatccg gactatcgaa gatccactgc
     2041 tcgacccgca ggaaattctt cagctgcgtc ggatcggagg cctgcgacag catctcgaca
     2101 tatttgtgct gcgtcagacg aaacggcatc aacgccagaa acaactgatt cagaatctca
     2161 cccgagacat tgccctcgcc gacgaacgcg gcgatgtcaa tcccgcgaac ccacttcgac
     2221 agcagatccg ccggcgtatg gaaatccacc ggggtcacca tggtgatcag attgctcacg
     2281 cgctgcggat agagcgcgcc atagcacaag ccgagcacgc ccccctgaca cacgcccagc
     2341 agattcagcg ccgagatctg atgggcgttc aacacgtgct cgacgcactc tgcgagcagc
     2401 cgatcgatat agtcggccag tgtcgtgaac cggtcggcgc catcaggata gccccagtcg
     2461 atcagataga cgtcgcggcc ggaagccagt aacccgcgga tcagcgagcg atcttcctgc
     2521 agatccatca tgtagggacg gttgaccagc gcgtagacga tcagcagtgg ggccgcaccc
     2581 aaggggcgcg tgatcggctg atagcgatac agcgtgagct tgccggattg ccagacgaca
     2641 tccttgtgcg agcagccggt catgttcgct tcttcttcgc tttcgccttc gctgttggcg
     2701 gtttcgaagg ggaatttcgg ggcggggcgc gtgtcgcccc gcctgagccg gacattgatc
     2761 gcagttgctc ctccgtcgcc cgcagacgac gggtcagcga gttgatctca ctgcgcgttg
     2821 gcaggtcgag gaacttggac cagtgctcga tattggcctg ctgctccaat cgccactgac
     2881 tggctgcgtt ggcaaactcg gcctgcgcct gacagaacgc ctcaccatgc gccacgcgcg
     2941 cgtaggcatc ctcagcgcag tcgatccatg tgtcatagag tctgcgcaga ctctctggcg
     3001 tgacggctgc cggcggcgac gctgagagct gcgacacgaa ccggctcgcc gcctcgcgca
     3061 gcacgtccga ccacagtcgc tgcaatcggt gctgcgcggc ctccatgcgc gccgcggcct
     3121 gcgccatgcg ctgcgcgcgt tgctgctgct cacgcgtcgc accgaacgct ggcacgtccg
     3181 gcgacaacgg ggaagacggc gacgaggccg ccgcaggcgc gaaggcactg ccccagggct
     3241 gcgaacccca gggctgcgaa ccccagggct gcgagtagct ggcgaactga tcgcgcagga
     3301 aatcgccgaa ctgggccgag gcgccctgcg cggatgcctt ggaaaggctg tccatgtagc
     3361 tgcgcgcggc atcgctgaag gcctgccatc caccccacgg atcggcgctc acgcttgcac
     3421 ccacgtatgc gctgcaagag agctccgatc ggcacgaaga tggccaccgg cccatccaat
     3481 ctcgacataa catactgttt caaaagagtt cataaatgca ccggttagtc aatccacacc
     3541 tcagaaagcc ccagagttgt gcattgcgaa agcttggcct agcatggctt acggatttaa
     3601 gagcgggatc atgccgtcgg aacgtctcat acgaaagtac tcgaatcgtc ggctgtacga
     3661 caccggagcg agtcggcatg tgacgctcga agatctgcga cagctgatca tctcgggcga
     3721 gaagatcaaa gtcgtagatg ataagaccgg cgaggacctg acccgcgcgg tgctgctgca
     3781 gatcatttcc gaacaggaac agttcggctc gccggtactg agcgccgaac tgcttgaaat
     3841 gatcatccgt ttctacggtc gcccgatgca ggcgatgctc agtcgttatc tggaacaatc
     3901 cttctcgatg atgctgcgcc agcaggaagt cgtgcaggct gagatgacca aagcactgca
     3961 gtccaccccg tttgctccgc tcaccgagct ggcgcgcaag aacatggaac tgtggacgga
     4021 aatgcagcgc gcgacgcgcg atgcgttcag cggccgcacg actgcgggct ccaatcccga
     4081 cgaccaagac cccgagggaa agtgaccaat ctgtatggac gtgcgcaaga aaaccattgt
     4141 tgtgacgggt gccggccgtg gcattggacg tgcgatcgcg ctgcagctcg ccgctcacgg
     4201 cgcgaacatc gcgatcttcg atctcaacag cgacgacatt gagcagacgc gctcgctatg
     4261 cgctgccaaa gcagtggatg cacgcggcta ccacgtcaac gtcgccgatg agaacgaagt
     4321 cactgccgcc atggcgacgg tcgtggcgga gtttggccgg ctcgatggcc tggtcaataa
     4381 cgccggcatc gtgcgcgatg ccctgctcat caaggtcaaa gacggcgcgg tggtggcccg
     4441 catgaccctg gcgcaatgga acgccgtcat tggcgtcaac ttgagcggcg tattcctgtg
     4501 cgcacgtgag gccgcggcgc agatgatcca gctcggcaac ggcggcgtga tcgtgaacat
//
LOCUS       DATMRM010000340        10389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_176292, whole genome shotgun sequence.
ACCESSION   DATMRM010000340 DATMRM010000000
VERSION     DATMRM010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10389
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176292"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3593
                     /locus_tag="VNZ06_10070"
     CDS             <1..3593
                     /locus_tag="VNZ06_10070"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWW21139.1"
                     /translation="QWQFWIDRGGTFTDVVAVKPDGALLTLKLLSDDPRRYEDAAVEG
                     VRRLMGVTADQALSASDIEQIRMGTTLATNALLERKGEPTVLIVTQGFRDALRIGYQN
                     RPRLFELHIHRPAELYARVIEVPERVDARGAILTALDLKAAEQSLRQAWQDGFRALAI
                     TLMHAYRYHAHELQLERLARAVGFTQISLSHKVSPLMRFVSRGDTTTVDAYLSPILRR
                     YVDQLASRLTGVELQFMQSHGGLTRADSFQGKDAILSGPAGGIIGMVRTAQSAGFSRI
                     IGFDMGGTSTDVSHFSGDFERQLETQIAGVRLTTPMLSIHTVAAGGGSILHFEEGRLR
                     VGPDSAGADPGPRAYRRGGPLTVTDCNVLLGKIQPAHFPQLFGKEGDEPIDTAGVREA
                     FTALAAQISAAIGRTRTPEQVAQGFIEIAVRNMANAIRRVSVERGYDVSDYVLNTFGG
                     AGGQHACLVADQLGMNRVFLHPLAGVLSAYGMGLADTSTMRERTAELELTDAALPVLE
                     SLLQQAEREARDELNGQGIAAARIRVLRKVHLRYQGTDTAVPVTFAGRESMQREFETL
                     YQQRFSFLLPERAIIAGVLTLEAIGVSERPTQAPLRAARHRDILAPLETVPVLYAAGW
                     LQTPLYRREGLRAADCIAGPAIIVEAHSTTVVEPGWQAQITPLDHLILSRVVARERGL
                     SATTRVDPMMLEVFANLFMAIAEQMGLRLQQSAHSVNIKERLDFSCAVFDRDGNLLAN
                     APHMPVHLGSMSESIKTVIRENAGRILPGDSFAINDPYRGGTHLPDITVITPVFASDQ
                     RSVLFYVGTRGHHADIGGITPGSMPSDSRHLDQEGVLFTNWKLVSAGRLLEAQTRALL
                     SSGPYPARNPTQNLADLRAQLAANAKGAQELLAMIEEFGSEVVQAYAGHVQDNAEEAV
                     RSVIGTLKNGSFRLETDAGSCIQVTVRVNAQARSAVIDFTGSSEQVADNFNAPASITV
                     AAVLYVFRSLVNRDIPLNAGCLRPLQIVIPEGSILNPRYPAAVVAGNVETSQCVTNAL
                     LAALGVCAASQCTMNNFSFGNDEHQYYETIAGGSGAGNGYAGTDAVQTHMTNSRLTDP
                     EVLESRFPVLLESFCIREGSGGAGRWRGGNGVARRLRFLAPMSASILSNNRRVAPFGI
                     RGGSAGACGSNSVQRRGGATERLSSCATVQMAAGEAFLIETPGGGGYGAA"
     gene            3672..4910
                     /locus_tag="VNZ06_10075"
     CDS             3672..4910
                     /locus_tag="VNZ06_10075"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW21140.1"
                     /translation="MRYNNTNSLSYGWVIVAVGALMTCVAVGAMFSLAVFLQPMSQVT
                     GWSRAGISAAMTLDFLSMGLAGFAWGTASDRFGARPVVLCGAVLLGTGLWLASRTRSL
                     TEFQLTYGLLVGVACGAFFAPMIATVTTWFDRRLSLAVSLVSVGVGVAPMTVSPLVRV
                     LISNYDWRFAMMSIGIVAWVLLIPAALFIRRAPARAGEPPAADEASTSGDAPIQSAAQ
                     ALRSPQFIVLAATYFGCCAAHSGPIFHMVSYAIGCGIPAMAAVSIYSVEGLAGLGGRL
                     LFGLAADRFGAKRILVGGLALQAVAICAYLSAGQLSQFYLLSVVFGTAYGGVMPLYAV
                     LARQSFAPRIMGTVLGAATLCSSFGMAFGPWAGGYVFDTFHSYQWLYLGSFAVALGAV
                     AIALAFPNRACATPAHMQPA"
     gene            5119..5769
                     /locus_tag="VNZ06_10080"
     CDS             5119..5769
                     /locus_tag="VNZ06_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWW21141.1"
                     /translation="MSKLRVLSFAVSLDGFAAGPHQDLQNPLGERGAELMEWFFNTRS
                     WRQMHGQPGGESGTDDGIAEQGFENVGAWILGRHMFGPVRGPWPDESWRGWWGEEPPY
                     HTQVFVLTHHARASLRMQGDTEFHFVTQGIHAALERARAAAGTRDVRLGGGVSTIRQY
                     LREGLIDELHLAVRPVLLGSGESLWRDLDMNALGYECWNSVAGERATHVYLRKRAR"
     gene            5766..6599
                     /locus_tag="VNZ06_10085"
     CDS             5766..6599
                     /locus_tag="VNZ06_10085"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HWW21142.1"
                     /translation="MIIHLIDGTYELFRQFYGTLRFAKAQDKPFAAVVGVLGGVLRMI
                     EDGATHLGVATDHVIESFRNELWSGYKTGEGIDPILFAQFHPLEDALQAMGVVIWPMI
                     ELEADDALASAATIAARDPRVQKISIWTPDKDLAQCVQGDRIVQVDAKSKTIRNADGV
                     RAKFGVRPELIPDYLALVGDSADGYPGIRGIGPKGAAGLLAKYGPLEAFPPNALGERQ
                     ELALLFKKLATLRTDAPLFGSVDELRWQGAGERFAACAQRMGAEPLIARAERAKAKLQ
                     L"
     gene            complement(6606..7052)
                     /locus_tag="VNZ06_10090"
     CDS             complement(6606..7052)
                     /locus_tag="VNZ06_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21143.1"
                     /translation="MRRLFNPYRLAAVVLVLNLLGHSFGALINTPDFGSESLRIAAIM
                     KSEHFQAQGFDVTWYGFYEGFGWFVSLFLAFSAWLAWTLGGWTAEFRRRLSAITLPLL
                     LSYVVDLVIVWKYFFPPPIIFTTIVVVLLGIGYWQDRRAVPAAGSP"
     gene            complement(7173..8147)
                     /locus_tag="VNZ06_10095"
     CDS             complement(7173..8147)
                     /locus_tag="VNZ06_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21144.1"
                     /translation="MRPTYAGALCLLLAVTGSARAAPESMEEAWWTGPLLAATASTLP
                     QGHGYFEPYLFDLIPYAHFDNHGHAHSVSHSNQFGSQSYLNYGLADGLTIGLIPRFGY
                     LRPVDAPSSDGVQVGDLTVQAQYRLSHFQPGNWLPTLSFNLQQVLPTGRYDHLDRLSD
                     GFGSGAYVTTPSIYSQAYFWMPNGRILRTRLNFSYSIASHVTLDDQSVYGTSAGFRGH
                     ATPGNSTFADLAFEYSATSHWVPALDVWYERDSSTGITGRYPSSAGSLASVALVSRSG
                     PGWELILAPALEYNWNARIGLIIGTRMIVAGSNETATLAPVIAFSYFH"
     gene            complement(8162..8284)
                     /locus_tag="VNZ06_10100"
     CDS             complement(8162..8284)
                     /locus_tag="VNZ06_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21145.1"
                     /translation="MSSAPAFQEHARLPHTRTFIERVCPLIDHRLDVVRATQVD"
     gene            8396..8572
                     /locus_tag="VNZ06_10105"
     CDS             8396..8572
                     /locus_tag="VNZ06_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21146.1"
                     /translation="MLESQRKNAISGCPLTAALAALGGKWKLLRAISHTVLPLLEQVR
                     LCGSEHSAQALHSH"
     gene            8586..9002
                     /locus_tag="VNZ06_10110"
     CDS             8586..9002
                     /locus_tag="VNZ06_10110"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005265768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWW21147.1"
                     /translation="MATTQEVAAFMSRAFPQSKCIVREVGARAATVSLPITDEQLRPG
                     GTVSGPTMMALADTALYVAILGEIGIVPLAVTTSLTINFLRKPAADKGIVAVCRLMKL
                     GKSLAVGEVAIYSEGEGEPVAHVVGTYSIPPRNHSD"
     gene            9046..9273
                     /locus_tag="VNZ06_10115"
     CDS             9046..9273
                     /locus_tag="VNZ06_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21148.1"
                     /translation="MEAFEELYCRISALELLVFAMAGQIDRTRFMQELALQKEKLLAT
                     ATFASLSTEVADRLTATIDRYARVLLQERRE"
     gene            9309..>10389
                     /locus_tag="VNZ06_10120"
     CDS             9309..>10389
                     /locus_tag="VNZ06_10120"
                     /inference="COORDINATES: protein motif:HMM:NF005686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWW21149.1"
                     /translation="MQGVALCAAIRSRQLSCAELMSACLDQIERLNPRFNAIVALQER
                     ALLLGLARQYDDELSGGQSRGPLHGVPFAVKDLELVNGIRTTFGSPLFKDFVPNTDSL
                     MVGRLRQAGAILIGKTNTPEFGLGSHTYNAVYGATRNAYDPSRSAGGSSGGAAVALAL
                     RMLPFADGSDYGGSLRNPAGWNNVFGFRPSIGRVPSDGRDLWLPSIGVLGPMARSVAD
                     LALLLSVQAGYDARAPLSIDGEGSLFCGPLEAELKGKRIAWGADLGGHTPCESGVLDL
                     CETALKTFESLGCVVEQAYPSYDLEALWRATISLRGWQQGAILLPFYRDPVRRALLTP
                     QALFEIETGLALSAYDISAASTIRTE"
BASE COUNT         1849 a         3208 c         3385 g         1947 t
ORIGIN      
        1 ggcagtggca gttctggatc gatcgcggtg gcacgttcac ggatgtcgtg gcggtgaagc
       61 ccgatggtgc gctgctcacg ctgaaacttc tatcggacga cccgcggcgc tatgaggatg
      121 cggccgtcga gggtgttcgc cggctgatgg gggtcacggc cgatcaggcg ctgtctgcga
      181 gcgacattga acagatcagg atgggcacga cgctggccac caatgcgctg ctggagcgca
      241 agggcgagcc gacggtgttg atcgtgacgc agggattccg ggatgcgctg cgcatcggct
      301 atcagaaccg tccgcggctg ttcgagctgc acatccatcg acccgcggaa ctctacgcgc
      361 gcgtgatcga agtgccggag agagtcgatg cgcgcggcgc gatcctgacg gcgctggact
      421 tgaaggctgc cgagcagtca ctgcggcagg cgtggcagga cggctttcgc gcactggcga
      481 tcacgctgat gcacgcctac cgctatcacg cacacgaact gcaactcgag cgcctggcca
      541 gagccgtcgg tttcacgcag atttcgttgt cgcacaaggt cagtcccctg atgcgcttcg
      601 tctcgcgtgg cgatacgacc acggtggatg cctacctgtc gccgatcctg cgccgttacg
      661 tcgatcagct cgcctcacgg ctgaccggag tcgaactgca gttcatgcaa tcgcacggcg
      721 gactcacccg cgccgacagc tttcagggca aggacgccat cttgtccggt ccggccggag
      781 gcatcattgg catggtgcgt accgcgcaat cggccgggtt cagcaggatc attggttttg
      841 acatgggcgg gacctccacc gacgtgtcac atttttccgg cgattttgaa cggcagctcg
      901 agacgcagat cgcgggcgtg cgactgacca cgccgatgct cagcattcac accgtggccg
      961 ccggaggcgg ctcgatcctg catttcgaag aggggcgtct gcgcgtcggc cctgattcgg
     1021 ccggggccga ccccggccca cgcgcctatc gtcggggcgg tccgctcacc gtcacggatt
     1081 gcaacgtgct gctgggcaag attcagccgg cccactttcc gcagctgttc ggcaaggagg
     1141 gcgatgagcc gatcgacacg gccggtgtgc gcgaggcgtt cacggcactc gccgcgcaga
     1201 tctcggcggc catcggacgc acgcgcacgc ctgaacaggt cgcacaggga ttcattgaga
     1261 ttgccgttcg caatatggcc aacgccatcc gacgggtatc ggtcgaacgt ggctacgacg
     1321 tcagtgacta cgtgctcaac acgttcggcg gagcgggcgg gcagcatgcc tgcctcgtcg
     1381 ccgaccagct cggcatgaat cgcgtgtttc tgcatcccct ggcgggcgtg ctgtctgcct
     1441 atggaatggg actcgccgac acgagcacga tgcgcgaacg caccgccgag ctcgagctga
     1501 cggatgcggc cttaccggtg ctcgagagcc tgttgcagca ggcggagcga gaggcgcgcg
     1561 atgaattgaa cgggcagggc attgccgctg cgcgcatccg cgtgttgcgc aaagtccatc
     1621 tgcgctacca gggtaccgat accgccgtgc cggtcacatt tgccgggcgc gagagcatgc
     1681 agcgggaatt cgagacgctc taccagcagc gcttttcctt tctgctgcca gagcgcgcaa
     1741 tcattgcggg cgtcctgacg ctcgaggcca tcggcgtgag tgagcggccc acccaggcgc
     1801 cgttgcgcgc cgcgaggcac cgcgacatcc ttgcaccgct cgagaccgta ccggtgttgt
     1861 acgctgccgg ctggctgcag accccgctct atcgacgcga gggactgcga gcagcggact
     1921 gcatcgcagg tcctgcgatc attgtggaag cccactccac gacagtggtc gagccaggat
     1981 ggcaggcaca gatcacaccg ctcgatcatc tgattctctc ccgggtcgtg gcacgcgagc
     2041 gcggcctgag tgcgaccacc cgcgtcgatc cgatgatgct cgaagtgttt gcgaatctct
     2101 tcatggcgat cgccgaacag atgggactgc ggctgcaaca gagcgcacat tcggtcaaca
     2161 tcaaggagcg gctcgacttc tcatgcgccg tgttcgatcg cgacggcaac ctgctcgcca
     2221 atgcaccaca tatgccggtg cacctcggct cgatgagcga aagtatcaag acggtcattc
     2281 gcgagaacgc cgggcgcatc ctccccggcg attccttcgc catcaatgat ccgtatcgcg
     2341 gtggcaccca cctgcccgat atcaccgtga tcacgcccgt gttcgcaagc gatcagcgat
     2401 cggtgctgtt ctatgtcggc acgcgcggcc atcacgccga catcggcggt atcacacccg
     2461 gctcgatgcc atccgactcc aggcacctgg atcaggaggg agtgctgttc accaactgga
     2521 agctcgtgtc ggctggccgg ctgcttgaag cgcagacccg cgcgctactg agctccggcc
     2581 cgtatccggc gcgcaacccc acccagaacc tcgcggacct gcgcgcgcag ctggccgcca
     2641 atgcgaaagg tgcacaggaa ctgctcgcga tgatcgaaga gttcggcagc gaggtggttc
     2701 aggcctatgc cgggcacgtt caggacaatg cggaggaggc cgttcgcagc gtcatcggta
     2761 cgctgaagaa tggatcattc cggctcgaaa ccgatgccgg atcctgtatt caggtcaccg
     2821 tgcgcgtgaa tgcgcaggcg cgcagcgcgg tgatcgactt taccggcagc agcgaacagg
     2881 tcgccgacaa cttcaacgct cccgcctcga tcaccgtcgc cgccgtgctc tatgtgttcc
     2941 gtagtctggt gaatcgcgat attccgctca atgccgggtg cctgcgcccg ctgcagatcg
     3001 tgattccaga aggctcgata ctcaatccgc gctatccggc cgcagtggtt gccggaaatg
     3061 tggaaacctc acagtgcgtc accaacgcgc tgctcgcagc gctgggtgtt tgcgcggctt
     3121 ctcagtgcac catgaataac ttcagtttcg gtaacgatga acaccagtac tacgagacca
     3181 tcgccggcgg ctcgggcgcc ggcaacggtt atgccggcac cgacgccgtg cagactcaca
     3241 tgaccaattc gcgcctgacc gatccagagg ttctggagtc tcgctttccg gtgttgctcg
     3301 agagtttttg cattcgcgag ggcagcggcg gagccggtcg gtggcgtggc ggtaacggtg
     3361 tcgcgcggcg tctgcgcttt ctggcgccga tgagcgcatc gatcctgtca aacaatcgtc
     3421 gcgtcgcccc gtttggtatt cgcggcggct ccgcgggtgc atgcggatcg aacagcgtgc
     3481 agcgacgcgg cggtgcaacc gagcggttat cttcctgtgc gaccgtacag atggcggcgg
     3541 gcgaggcgtt cctgatcgag acgccaggcg gcggaggcta cggtgcggcg tagtggaagg
     3601 cttcggttgc ggggaacgac ggtcctcgta tagcatacca actggtcggt acggacgcgg
     3661 tgcaaggggt catgcgctac aacaatacga actcactgtc ctatggctgg gtcatcgtcg
     3721 ctgtcggggc gctgatgacc tgtgtcgcgg tcggagccat gttctcgctc gcggtgtttc
     3781 tacagccgat gtcgcaggtc acgggctggt cacgggccgg catctcggcg gcgatgaccc
     3841 tcgacttcct gtcgatgggg ctggccggat tcgcttgggg cacggccagc gatcgcttcg
     3901 gtgcgcgccc ggtcgtgctg tgcggagcgg tgttgctcgg caccgggctg tggctggcca
     3961 gtcgtacccg ctcgctcacc gaatttcagc tcacctatgg cctgctggtg ggcgtggctt
     4021 gcggcgcttt tttcgcgccg atgattgcga cggtcacgac ctggttcgac cgacgtctga
     4081 gtctcgcggt ctcactggtg tcggttggag ttggcgtcgc gccgatgacc gtgtcaccgc
     4141 ttgtacgcgt gctcatctcc aactacgact ggcgcttcgc gatgatgagc atcggcatcg
     4201 tggcctgggt gctgctgatt cctgcggcac tgttcatccg ccgcgctccg gcccgagcgg
     4261 gcgaaccgcc ggcagccgat gaggcttcta cttccggcga tgccccgatc cagtccgcgg
     4321 cgcaggctct gcgatcgcca caattcatcg tcctggctgc cacctatttc ggctgctgtg
     4381 ccgcgcactc gggcccgatc tttcacatgg tcagttacgc catcggttgc ggcatacccg
     4441 ccatggcggc cgtcagcatt tatagcgtcg agggtctggc cggtcttggt ggccggttgc
     4501 tgttcggtct ggcggccgac cggttcggcg ccaagcgcat tctcgtgggc ggcctcgcgt
     4561 tgcaggcggt cgcgatctgc gcgtacctca gcgccggcca actcagccag ttctatctgc
     4621 tgtcggtcgt cttcggcacc gcctacggtg gtgtgatgcc gctgtatgcg gtgctggcgc
     4681 gtcagtcgtt tgccccgcgc atcatgggga ccgtgctcgg tgcggccacg ctgtgttcaa
     4741 gctttggcat ggctttcgga ccgtgggccg gcggctacgt gttcgatacc ttccactcct
     4801 atcaatggct gtacctggga tcgttcgcgg tcgctctggg cgcggtggca atcgcactgg
     4861 ccttcccgaa ccgcgcctgc gccactccgg cgcatatgca gcccgcctga gcacgcatcg
     4921 cagcgctcgc ccggcagtta acgcagccga ttctgtcggg tgatatattc tataattcat
     4981 tagcttatac actatactta atgttaccag tatggccgcc agaaccgcat ggcggatgaa
     5041 atcggcatca ctcgagccgc actgaagacc ttaagatccc aggcaacctc ccgttcccgt
     5101 tccgagccag gagttgccat gtcgaaactt cgtgtcttga gcttcgcggt ctcgttggac
     5161 ggattcgccg ccgggccgca ccaggatctg caaaacccgc tgggtgagcg tggcgcggaa
     5221 ctcatggaat ggtttttcaa tacgcgcagc tggcggcaga tgcacggaca gccgggcggg
     5281 gaaagcggta ccgacgacgg catcgccgag caggggttcg agaacgtcgg ggcctggatc
     5341 ctcggacgtc acatgttcgg gccggtgcgt ggaccgtggc ctgatgagag ctggcgaggc
     5401 tggtggggcg aggagccgcc gtatcacacc caggtatttg tgctcaccca tcacgcgcgt
     5461 gcgtcgctga ggatgcaggg cgacactgaa tttcatttcg tcacgcaggg cattcacgcg
     5521 gccctcgagc gcgcccgggc ggccgccggc actcgcgatg tccggctcgg aggtggcgtt
     5581 tccacgatcc ggcaatatct tcgcgaaggc ctgatcgatg aactgcacct ggccgtgcga
     5641 cctgtgctgc ttgggagcgg tgagtcgctg tggcgcgatc tggacatgaa cgcgctgggc
     5701 tatgaatgct ggaacagcgt cgcgggagag cgcgccacgc acgtctatct gcgcaaacgc
     5761 gcacgatgat catccacctg atcgatggca cctacgagct gttccgccag ttctatggaa
     5821 cgctgcgctt cgccaaagca caggacaagc cgtttgcggc cgtcgttggc gtgctcggtg
     5881 gcgtgcttcg catgatcgag gacggcgcca cgcacctggg agtggcgact gaccacgtga
     5941 tcgagtcctt ccgaaacgaa ctgtggagcg gctacaagac cggcgagggt atcgatccga
     6001 tcctgtttgc gcagtttcat ccgctcgagg acgcgctgca ggcgatgggc gtggttatct
     6061 ggccgatgat cgaactcgaa gccgacgacg cattggcatc ggccgcgacc attgccgcgc
     6121 gcgatccgcg cgtgcagaag atctcgatct ggacgccgga caaggacctt gcgcagtgcg
     6181 tgcagggtga ccgcatcgta caggtcgatg cgaagagcaa gaccattcgc aacgcggacg
     6241 gcgtacgcgc caaattcggc gtgcgcccgg aactgatccc cgactatctg gcgctcgtgg
     6301 gcgacagcgc ggacggatac cctggcatcc ggggcatcgg tccaaagggg gccgccggtc
     6361 tgctggcgaa gtacggtccg ctggaagcct tcccgccgaa cgcgctcggt gagcggcagg
     6421 aattggctct gctctttaag aagctcgcca cactgcgcac cgatgcgccg ctgtttggca
     6481 gtgtggatga gcttcgctgg cagggcgcgg gtgaacgctt tgcggcctgc gcgcaacgga
     6541 tgggcgccga gccgctgatc gcacgcgccg agcgggccaa agcgaaactg cagctgtgag
     6601 tggtgctatg gggatccagc cgctggtacc gcacgacgat cctgccagta gccgatgccc
     6661 agcaacacga ccacgatggt cgtgaagata atcggtggcg ggaagaagta cttccagacg
     6721 atcaccaggt caaccacgta gctcagcagc agcggcaaag tgatggcgga cagacgtcgc
     6781 cggaactcgg ccgtccagcc cccgagcgtc caggccagcc atgccgaaaa ggccagaaac
     6841 agcgagacga accagccaaa cccttcatag aacccgtacc aggtcacgtc gaatccctgc
     6901 gcctggaaat gttcggactt catgattgcg gcgatacgca ggctctcgct gccgaagtcg
     6961 ggtgtattga tcagcgcgcc aaagctgtgg cccagcagat tcagcaccag cacgacggct
     7021 gccaaacggt acggattgaa tagtcgcctc atggttgtga agtgcccagg gcaccctccc
     7081 gcagttttta ttggaattcg agcgcaacaa acgaagttct gccttcgtct ggcactgatc
     7141 gtgtggaccg gaccttagca gaattgcacc cgctaatgga aatagctaaa agcgatcaca
     7201 ggggcgaggg tcgccgtctc gttgctgcct gcaacgatca tccgggtacc gatgatgagg
     7261 ccgatccttg cattccagtt gtactccagc gccggcgcca gaatcagttc ccatcccgga
     7321 ccactgcggc tcaccagggc aaccgatgcc agcgaacccg cagaggatgg atagcggcct
     7381 gtgatgccgg tgctcgaatc gcgttcgtac cacacatcca gcgccgggac ccagtgactg
     7441 gtggcactgt actcaaaggc cagatcggcg aaagtcgagt tacccggtgt ggcatggcca
     7501 cgaaaaccgg cgctggttcc gtagacactc tgatcgtcca gcgtcacatg gctggcgatc
     7561 gaataagaga aattcaggcg cgtgcgcaga atgcgtccgt tcggcatcca gaaataggcc
     7621 tgcgaataga tcgacggggt cgtgacgtag gcgccactgc cgaagccgtc actgagcctg
     7681 tccagatgat cgtagcgacc ggtcggcagc acctgctgca gattgaacga cagcgtcggc
     7741 agccagttcc cgggttgaaa gtgcgataat cggtactgag cctgcacggt caggtcgccc
     7801 acctgcaccc cgtcgctgct gggtgcatcg accggccgca gatagccaaa tcgcggtatc
     7861 agaccaatgg tcaggccatc ggcgagtccg tagttgagat acgactgcga gccgaactgg
     7921 ttgctgtggg acacactgtg cgcgtggccg tggttgtcga aatgcgcata ggggatcaga
     7981 tcgaacaggt acggctcgaa gtagccgtga ccctgcggca gggtcgaggc cgtagccgcg
     8041 agcagcggtc cggtccacca tgcctcctcc atcgactccg gcgcagcgcg ggcgctgccc
     8101 gtcacagcca gcagcaggca gagcgcaccc gcgtaggtcg gtctcacggc gggcgcgctc
     8161 ttcaatccac ttgcgtcgct cgcacgacgt ccagtcggtg gtcgatgagt gggcatacgc
     8221 gctcgatgaa cgtgcgcgta tgcggcagtc gggcgtgctc ctggaatgcc ggtgcgctcg
     8281 acatcgattt ccttatctaa ccagcgcgta caagcgggcg aagatgacaa gcttcatgga
     8341 ccggctagag tagtgctccg gctacgcacg tgccagaagg caccttgagc caattatgct
     8401 tgaatctcaa cgcaagaatg cgatatcggg atgtccgttg acggccgcac tggcggcact
     8461 gggcggcaag tggaaactgc tgcgggcgat ctctcacacg gtgctgccac tgctggaaca
     8521 ggtgcgcctg tgcggtagcg agcacagcgc gcaagccctg cacagccact agacaggatc
     8581 ccgacatggc aaccactcag gaagtcgccg cgttcatgag ccgcgcgttt ccgcaatcga
     8641 agtgcatcgt gcgagaggtc ggcgctcgcg cggccacggt gtcgttgcca atcaccgacg
     8701 aacagctgcg cccgggtggt acggtgtccg gtcccaccat gatggcgctg gccgatactg
     8761 cgctgtacgt ggcaatactg ggggagatcg gcatcgtgcc gctggccgtc accaccagtc
     8821 tcaccatcaa tttcctgcgt aagccagcgg ccgacaaggg catcgtggcc gtgtgcaggc
     8881 tgatgaagct cggtaagtcg ctagcggtcg gcgaggtagc gatctactcg gagggtgagg
     8941 gcgagccggt cgcacatgtt gtgggcacct actccatccc gccgcgaaac cacagcgact
     9001 gacacagcgc gatccggcgt ccggggttgt tttatgattc gcagcgtgga agccttcgaa
     9061 gagctctatt gccggatcag cgcgctggaa ctgctcgtgt tcgccatggc cgggcaaatc
     9121 gaccggacgc gcttcatgca ggaactggcc cttcagaagg aaaagctact ggcgacggcg
     9181 accttcgcct cgctgtccac cgaggtggca gatcgactga cggcgacgat cgaccgctac
     9241 gcgcgagtgc tgctgcagga gcgccgggag taggcagtgt gaattcgggt gaggccgatc
     9301 cgctcctgat gcagggcgtg gcgttgtgcg cggcgattcg gtcgcggcag ctctcatgtg
     9361 ccgagctcat gagcgcctgt ctggatcaga tcgagcgcct caatccgagg ttcaatgcca
     9421 tcgtcgcgct acaggagcgt gcactgctgc tgggactggc acgacagtac gatgacgagc
     9481 tcagtggtgg acagtcgcgc gggccgctgc atggcgtacc cttcgcagtg aaggatctgg
     9541 aactggtgaa cggcattcgc accacgttcg gatcgccact cttcaaagac ttcgttccaa
     9601 ataccgacag tctcatggtg ggtcggttac gtcaggctgg cgccatcctc atcggtaaga
     9661 ccaatacacc cgaatttgga ctgggctcgc acacctacaa cgcggtgtat ggggcgaccc
     9721 gcaacgccta cgacccctcg cgctccgccg gcggcagcag tggcggggcg gccgtggcac
     9781 tggcgctgcg catgctgccc ttcgcggacg gatcagacta cggtggcagt cttcgcaatc
     9841 cggcaggatg gaacaacgtc ttcggatttc gacccagcat cggacgcgtg ccgtccgatg
     9901 ggcgcgatct ttggctgccg tcgatcggag tgctcggacc gatggccagg agcgtcgcgg
     9961 atctggcctt gctgctgtcg gtgcaggcag gctatgacgc acgggcgccg ctgtcgatcg
    10021 acggcgaggg ctcgctgttt tgcggcccgc tcgaggccga gctcaaggga aagcgtatcg
    10081 cctggggcgc ggatctgggt ggtcatacgc cgtgcgagag cggggtgttg gatctgtgcg
    10141 agacggccct caagactttc gagtcattgg gctgtgtggt cgagcaggcc taccccagtt
    10201 acgacctcga ggcgctgtgg cgtgcgacga tcagcctgcg cggctggcag cagggtgcca
    10261 tcctgctgcc gttctaccgg gacccggtcc gacgcgcgct gctcacaccc caggcgctgt
    10321 tcgaaatcga gactgggctg gcgctctcgg cctacgacat cagcgccgcc tcgacgatac
    10381 gcaccgagt
//
LOCUS       DATMRM010000341         5940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_824418, whole genome shotgun sequence.
ACCESSION   DATMRM010000341 DATMRM010000000
VERSION     DATMRM010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824418"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VNZ06_10125"
     CDS             complement(<1..670)
                     /locus_tag="VNZ06_10125"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HWW21150.1"
                     /translation="MNSVVKLAMRDINSIPFQEASLDIWDKKYRLTSKDGQALDGSMD
                     DTYKRVARALADVEDAERRDQWYERFLWALRHGAIPAGRVTSNAGAQEHKPATSTINC
                     TVSGTVHDSMDDILNKVHEAGLTLKAGCGIGYEFSTLRPRGAYVSGAGSYTSGPLSFM
                     DIYDRMCFTVSSAGGRRGAQMATFDVGHPDAIDFVRAKRENGRLRQFNLSLLVTDEFM
                     KAVRE"
     gene            1110..1550
                     /locus_tag="VNZ06_10130"
     CDS             1110..1550
                     /locus_tag="VNZ06_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWW21151.1"
                     /translation="MTLLRYEPWGVVARLQRHFDQAFADIDGSASNGESHAAWIPSVD
                     IHQESDKFVVRADLPGVEPKDIDVTAENGVLTVSGERKFERSEQQKGTSRLERVEGRF
                     VRRFTLPENVQTDHIRARHANGVLELTIPKAAAPEPKKISVETH"
     gene            complement(1552..2589)
                     /locus_tag="VNZ06_10135"
     CDS             complement(1552..2589)
                     /locus_tag="VNZ06_10135"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HWW21152.1"
                     /translation="MLTERRIVAVILMLGALTGCTSLGYLAQAAHGQFQLMRTRRPIE
                     QVIADPASNGALKTRLRLVEQARDFAVTDLKLPDNRSYRTYGDLGRPYAVWNVVAAPE
                     FSVTPLHWCFPFTGCISYRGYFHERSARKFAANLAGRGNDTLVAGVTAYSTLGHFADP
                     VTNTMLRYGDNDMIATIFHELAHQLIYVPGDSQFSESFAMSVELEGLRRWLSARERSD
                     ELQMILARHRDEQTIVKAFGEGRRQLAALYAQPVPVEQMRTQKQEILKVTGDHVLDLE
                     HQFHLPMYDLWINAGLNNAHLASIGTYYDCVPGFQRLLEANGGDLPSFYAAVKKMRRD
                     VSARRSLCSSR"
     gene            2598..3680
                     /gene="cca"
                     /locus_tag="VNZ06_10140"
     CDS             2598..3680
                     /gene="cca"
                     /locus_tag="VNZ06_10140"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645737.1"
                     /note="catalyzes the addition and repair of the essential
                     3'-terminal CCA sequence in tRNAs without using a nucleic
                     acid template; phosphohydrolase activities include
                     hydrolysis of pyrophosphate, 5'-nucleoside tri- and
                     diphosphates, NADP, and 2'-AMP with the production of Pi,
                     metal-dependent phosphodiesterase activity for 2',3'-cAMP,
                     2',3'-cGMP, and 2',3'-cCMP, and hydrolysis 2',3'-cyclic
                     substrates with the formation of 2'-nucleotides and
                     3'-nucleotides; these phosphohydrolase activities are
                     probably involved in the repair of the tRNA 3'-CCA
                     terminus degraded by intracellular RNases; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HWW21153.1"
                     /translation="MQVYLVGGAVRDELLGRPVHERDWVVVGATPEQMQQAGYRPVGR
                     EFPVFLHPQTHEEYALARRERKTGPGYRGFVTEFSPSVTLEQDLLRRDLTINAIARDS
                     NGTLIDPYGGVSDLEQRLLRHVSPAFVEDPVRILRVARLRARLASLGFQVAEETGALM
                     HEMVRNGEVNALVPERVWRETERALGEANPEVFFDILARCDALYVLFPGLSWSDDDRL
                     ALARAAALSQDGSVRFAALGAVVPAKALAALCDRLRAPAAYRDVARLCAQLTAQSRAG
                     LGNDPASTLLRLFEQADAFRRRERFEKVLLAAQARAKVDSRIPAALSAVAEVALAPDQ
                     MAAMHGADIASALHAARLERLRSFCG"
     gene            complement(3688..4848)
                     /locus_tag="VNZ06_10145"
     CDS             complement(3688..4848)
                     /locus_tag="VNZ06_10145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWW21154.1"
                     /translation="MFPALSPDMAEHSARVAAAVRARIQAGNGWLAFDEYLRLVLYAP
                     GLGYYSAGAEKFGPGGDYVTAPEISALFGHGIARQCAQVLEVSGGGVLELGAGSGALA
                     ESILMCLQEMQCLPAHYDILEISADLRQRQQQRLAALPASLRACIRWLDALPTQPVSG
                     VILANEVADALPFKRFVIGADAVLERGVGLSPSGHLIEFDRAADVLLSREITRIATDL
                     PDAWTPGLRSEVCLLIEPWIAALSAALARGTLLLVDYGSCRREYYHPQRAQGTVRCHF
                     RHRAHDDVLLYPGLQDITAWVDFTRVAEAATAAGLTVSGYATQAAFLLANGIEQELAS
                     VAAPVEHAKLASQARMLLLPGEMGESFKVIALTRGVDAPLRGFAYQDLRGRL"
     gene            4884..5642
                     /locus_tag="VNZ06_10150"
     CDS             4884..5642
                     /locus_tag="VNZ06_10150"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HWW21155.1"
                     /translation="MSKERPPSDRLRGRTVLITGGARRIGAHLARALHAAGANVVIHC
                     HRSRAEADDLSAELEAVRPASTAVLSADLLDVAQLPPLVRATCDRFGTLDVLINNASS
                     FYPTPIGSIELKAWDDLLGTNLRAPTFLTQAAAPELRRAQGLVLNLVDIHGLRPLREH
                     SVYSTAKAGLIMLTRALARELAPEVRVNAIAPGPVLWPESGIGQERQQKILAQTPLQR
                     PGSPEDIVRAVLFFATDAPFVTGQILAVDGGRSI"
     gene            complement(5659..>5940)
                     /locus_tag="VNZ06_10155"
     CDS             complement(5659..>5940)
                     /locus_tag="VNZ06_10155"
                     /inference="COORDINATES: protein motif:HMM:NF013456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWW21156.1"
                     /translation="IEARCGRTPAAPKWAPRAMDLDLLLYGELIGRGPGYTLPRPDLL
                     KRAYMLGPLAQLAPDLRYPPSGATIGQLWAQFPQAEGALTALELDLNAA"
BASE COUNT         1100 a         1903 c         1860 g         1077 t
ORIGIN      
        1 cttcgcgcac cgccttcatg aactcgtcgg tgaccagcag cgacagattg aattgccgca
       61 gccgcccgtt ttcgcgcttg gcgcgcacga aatcgatcgc atcgggatgg ccgacatcga
      121 aggtcgccat ctgcgctcca cgacggccgc ccgccgagga caccgtgaag cacatgcggt
      181 cgtagatatc catgaacgac agcgggccgg aggtatagga gccggcgccc gacacgtacg
      241 cgccgcgcgg gcgcagcgtc gaaaattcat agccgatgcc gcatcctgcc ttcaacgtca
      301 ggcccgcctc gtgcaccttg ttgaggatgt cgtccatcga gtcatggacc gttccggaca
      361 cggtgcagtt gatggtcgag gtcgcgggct tgtgctcctg cgccccggca ttcgaggtca
      421 cgcgaccagc cgggatggcg ccgtgacgta gcgcccatag aaagcgctcg taccactgat
      481 cgcgacgctc ggcgtcttcg acatccgcca gcgcgcgggc gactctctta taggtgtcgt
      541 ccatcgagcc atcgagtgcc tggccgtcct tcgaggtcag ccggtacttc ttatcccaga
      601 tatccagcga ggcttcctgg aagggaatcg agttgatgtc acgcatggcg agcttcacaa
      661 cggaattcat attggcggcg actcccctat tgcgcaaaat cggcgaccct gtcagtccac
      721 tgtcgaaaca caatatcttg ttgtgtgagg gagcgagact aatcaagctc ggagggcatc
      781 gtcaagatta tgaaataatt tattcttgtc tattttatag atataaatca gtatcttata
      841 ccataatccg ctgcatcttc ttgatcaata tctgagcact tccggcagat tttcttgccc
      901 ggctcagacc ccaagatctt gtgttcgtcc tatccaccag aggctcacag ctcacccaca
      961 ggctatgcac agcaggcgct tgcgggcgga tttccctggc agggcttgac acgggtcttg
     1021 aaacgcggcc gcgcaccctc agattgagtg catgcgatcg ggcggtaagg cagcccgaaa
     1081 gtttcacaaa ccctacggag tatctgacca tgacactgtt acgttatgaa ccctggggcg
     1141 tcgtcgcacg actccagcgg cattttgatc aggctttcgc cgatatcgac ggctcagcga
     1201 gcaatggcga gagccatgcg gcgtggattc cctcggtcga cattcaccag gaatccgaca
     1261 agttcgtggt tcgcgcggac ctgccgggcg tggaaccgaa ggacattgat gtgaccgctg
     1321 agaacggcgt gctcaccgtc agcggcgaac gcaagttcga gcgcagcgaa cagcagaagg
     1381 gcacgtcgcg tctagagcgc gtcgaaggcc gtttcgtccg tcgcttcacg ctgccggaaa
     1441 atgttcagac cgaccatatc cgggcccgtc acgccaatgg cgtgctcgag ctgacgattc
     1501 cgaaggctgc agcgccggag ccgaagaaga tctcggtcga gacgcactaa atcatcgact
     1561 cgagcacaag ctgcgccgcg cgctcacgtc gcggcgcatt ttcttgaccg ctgcataaaa
     1621 agagggcaga tcgccgccgt tggcttcgag caggcgctga aaaccgggca cgcagtcgta
     1681 gtaggttcca atggacgcca gatgcgcgtt gttgaggccc gcgttgatcc acaggtcgta
     1741 catcggcagg tgaaactggt gctcgaggtc gagcacgtga tcgcccgtga cctttagaat
     1801 ttcctgtttc tgcgtgcgca tctgctccac cggcacaggc tgtgcgtaca gtgctgccag
     1861 ctggcgccgt ccttcaccaa aagccttcac gatcgtctgt tcgtcgcgat ggcgggccag
     1921 gatcatctgt agctcgtcgc ttcgctcgcg cgccgagagc cagcgacgca gcccctccag
     1981 ttccacgctc atggcaaacg actcgctgaa ctggctgtcg cccggaacat agatgagctg
     2041 atgcgcgagt tcatgaaaaa tcgtcgcgat catgtcgttg tcgccatagc gaagcatcgt
     2101 gttggtcacc gggtcggcga aatgcccgag tgttgagtac gcggtcacac ccgctaccag
     2161 cgtatcgtta ccgcgccccg ccaggttcgc cgcaaacttg cgggcgctgc gttcgtggaa
     2221 gtagccgcga tagctgatgc agcccgtgaa cggaaagcac cagtgcagcg gcgtcacgga
     2281 gaactcgggc gcggccacga cattccagac cgcatatggg cgacccagat ccccataggt
     2341 gcggtagctg cgattgtcgg gcagcttcag gtcggtcacg gcaaagtcgc gcgcctgctc
     2401 gaccagcctc aggcgcgtct tcagcgcacc attgcttgcc ggatcagcga tgacctgttc
     2461 gatcggccga cgcgtgcgca tcagctgaaa ctggccgtgc gcggcctgcg ccagatagcc
     2521 cagcgaggtg cagcccgtca gtgcgccgag catcaatatc acggcgacga ttcgtctctc
     2581 ggttaacatg cgcgtccatg caagtctatc tcgtgggcgg cgcggttcgc gacgagctac
     2641 tcgggcgccc ggtgcacgag cgtgactggg tcgtggtcgg ggcaacccct gagcagatgc
     2701 aacaggcggg ctaccgtccg gtcggacgcg aatttccggt gttcctgcat ccgcagacgc
     2761 acgaggagta cgctctggcg cgccgggagc gcaagacggg tcccggatat cgcggcttcg
     2821 tcaccgaatt ctcgcccagt gtcacactgg aacaggatct gctgcggcgt gatctgacca
     2881 tcaacgcgat cgctcgcgat tccaacggca cgctcatcga cccgtacggc ggcgtgagtg
     2941 acctcgaaca gcgcttgttg cgacacgtct caccggcatt cgtcgaagac ccggtcagga
     3001 tcctgcgcgt ggcgcgcttg cgtgcgcggc tcgcatcgct gggattccag gtggcggagg
     3061 agaccggtgc gctgatgcac gagatggtac gcaacggcga ggtcaacgcg ttggtgccgg
     3121 aacgggtctg gcgtgagacc gaacgagccc tgggcgaggc gaatcccgag gtgttcttcg
     3181 acattctggc gcggtgcgac gctctgtatg ttctgtttcc cggattgtcg tggtcagatg
     3241 atgatcgtct cgcactggca cgcgccgctg cgctgagcca ggatggctca gtgcggttcg
     3301 cggcgctcgg cgctgtggtt ccggcgaagg ctctcgcagc gctctgcgat cgtctgcgag
     3361 cgccggccgc gtaccgcgac gttgcgcgac tgtgtgcgca gctcacagcg cagtcgcgcg
     3421 caggtctggg caatgatccg gccagcacac tgctgaggct gttcgaacag gctgacgcct
     3481 tccgccgccg ggaacgattt gaaaaggtgt tgctggcggc gcaggcccgc gcgaaagtcg
     3541 actcccgcat ccctgccgca ctgtctgccg tggccgaggt cgcgctggcg ccggatcaga
     3601 tggcggccat gcacggtgcg gacatcgcaa gcgctctgca cgctgcgcgg cttgaacggc
     3661 tgcgcagctt ctgtggctga cggggcctta caggcgcccg cgcaaatcct gataggcgaa
     3721 tccacgcaac ggcgcgtcca ctccacgtgt caatgcgatg accttgaagc tctctcccat
     3781 ctctccgggc agcagcagca tacgggcctg cgatgcgagc ttcgcatgct cgaccggtgc
     3841 ggcaaccgag gcgagttcct gctcgatacc attggcgagc agaaatgccg cttgcgtcgc
     3901 atagccactg accgtaagcc ccgcggcagt agcggcttcc gcgacccgcg tgaagtccac
     3961 ccacgcggtg atgtcctgca gccccggata cagcaacacg tcatcatgcg cgcgatggcg
     4021 gaagtggcag cgcaccgtcc cctgtgcgcg ctgcggatgg tagtactcgc gcctgcaact
     4081 accgtagtcc acaagcagta gcgtgccgcg cgccagcgcg gccgataacg ccgcgatcca
     4141 cggttcgatc aacaggcaga cctccgaacg caagcctggc gtccaggcat ccggcaggtc
     4201 agtcgcgatc cgtgtgattt cccgagagag cagtacatcg gccgcccgat cgaactcgat
     4261 cagatgtccc gagggtgaaa ggcccactcc acgctcgagc acggcgtcag cgccaatcac
     4321 aaagcgcttg aaaggcaatg catcggcgac ctcgttggcc agaatcacgc cgctgactgg
     4381 ctgcgtgggc agcgcatcga gccatcggat gcacgcgcgc aacgaggccg gcagcgcggc
     4441 cagccgttgc tgctgccgct gtcgcagatc ggcactgatt tccagaatgt cgtagtgcgc
     4501 aggcaggcac tgcatttcct gaaggcacat caggatgctc tcagccagcg ccccgctgcc
     4561 cgcaccgagc tcgagcacac cgccaccgct gacttcgagc acctgcgcgc actggcgggc
     4621 gatgccatgc ccaaacagcg cggaaatctc cggcgcggtc acatagtcac cgcccggtcc
     4681 gaatttttcg gcgcccgcgc tgtagtagcc aagcccgggc gcgtacagca ccaggcgcag
     4741 atactcatcg aacgcgagcc agccgttgcc ggcctggatg cgtgcacgca ccgctgcggc
     4801 tacccgggcg ctatgctcgg ccatgtcggg ggacaacgca ggaaacattg agactgactc
     4861 gcgcatcgtc ataagctgca ccgatgagca aggagcgacc cccctctgac cggctgcgcg
     4921 gccgtacggt attgatcacc ggcggtgcgc ggcgcatcgg cgcccacctc gctcgcgcgc
     4981 tgcacgccgc cggcgccaac gtcgtgatcc actgccatcg ttcccgcgcc gaggccgacg
     5041 acctcagcgc cgaactggaa gcggtgagac ccgcctcgac ggcggtgttg agcgccgatc
     5101 tgctcgacgt ggcacagctg ccaccgctgg tgcgtgccac ctgtgaccga ttcggcacgc
     5161 tggacgtgct catcaacaat gcttcgagct tctacccgac gcctattggc agcatcgagc
     5221 tcaaggcctg ggacgatctg ctcggcacca acctgcgcgc gccaaccttc ctcactcaag
     5281 cggcggcgcc ggagttgcga cgagcgcagg ggctggtatt gaatctggtg gatatccacg
     5341 ggctgcggcc actgcgtgaa cattcggtct attcgactgc caaagccggg ctcatcatgc
     5401 tgacgcgcgc gttggcgcgt gagctggcac ctgaagtgcg cgtcaacgcg atcgcaccgg
     5461 ggccggtgct gtggccggaa agcggtatcg gccaggagcg gcaacagaaa attctcgcgc
     5521 agacgccact gcagcgcccc ggttcgccgg aagacatcgt tcgtgcggta cttttcttcg
     5581 cgaccgatgc gccgttcgtc acgggacaga tcctggcggt ggatggcggt cgcagcatct
     5641 agccatcgcg cgcttggctt aagcggcatt caggtccagc tccaaggcag tgagtgcgcc
     5701 ctcggcctgc gggaattgcg cccacagctg gccgatcgta gctcccgaag gcgggtatcg
     5761 cagatccggg gccagttgtg ccagcgggcc gagcatatag gcgcgcttca acaggtcggg
     5821 ccggggcagc gtataccccg ggccgcggcc gatgagctca ccgtagagca gcaggtcaag
     5881 gtccattgcc cgcggcgccc atttgggtgc ggcgggcgtc cgtccacaac gcgcctcgat
//
LOCUS       DATMRM010000342         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2045694, whole genome shotgun sequence.
ACCESSION   DATMRM010000342 DATMRM010000000
VERSION     DATMRM010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2045694"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /gene="def"
                     /locus_tag="VNZ06_10160"
     CDS             complement(<1..485)
                     /gene="def"
                     /locus_tag="VNZ06_10160"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWW21157.1"
                     /translation="MALLPVLEFPDPRLRTRAKRVEEFDAGLKQLVADMLETMYAAPG
                     IGLAATQVNVHRQVVVIDIGGDSRAPQVYINPEILTRDGVEVSEEGCLSVPNVFEQVE
                     RAACVRVRAQDVDGQTFERDLDGLGAVCLQHEIDHLAGKLFVDYLSVLKRERIRRKLE
                     K"
     gene            772..1923
                     /locus_tag="VNZ06_10165"
     CDS             772..1923
                     /locus_tag="VNZ06_10165"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWW21158.1"
                     /translation="MFHHETAQSAVPPPATQVAQTDTTNASAPELQTVTDALSDTDEA
                     GTTTVMRDAGPALRAGAPKDYVVKRGDTLWGIANMFLKDPWLWPEIWYVNPDIHNPHR
                     IYPGDTVHLALGGDGRTALQVVRGSTVGGTRLEPLLRSSPLEGPIATIPYSAIAAFLS
                     RPGVMTREEVKAAPYVVALRDEHQVAGAGHDLYVKKLSGDAGTRYNVMHIDEPLKDPQ
                     SGRHLGYMAIYTGTAQLTRSGPIARATLVDSARETLQGDVLIAQTTNPTSDFLPHPPS
                     QPIDGRIIAVVDNVLLAGQYEIAALNRGSEDGLDRGTVLTIEEGQRVATDHCAHIDGS
                     STCFAGHSTQLPIETEGSLLVFKTYEKMSYALILSSTVPIHMNDKVRNP"
     gene            2020..>2804
                     /gene="dprA"
                     /locus_tag="VNZ06_10170"
     CDS             2020..>2804
                     /gene="dprA"
                     /locus_tag="VNZ06_10170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HWW21159.1"
                     /translation="MDAIDLQLALGRARGLTASKLRRYGATLGECAGQRGLPALFEQS
                     PPVRTALMAVDATLIAADRRWIQRESISLIDLTSPGYPSLLAQLRDAPALLYVKGDVA
                     ALSTPQLAIAGTRKASAPAQLTARNFAARLAREGLTVTSGLALGIDAASHEGALHVGG
                     RTIAVLGTGLDQIYPATHRVLAQRIAAQGALVSELPPGVPPLASNFPRRNRIISGLSL
                     GVLIVEAARDSGSLITAALARDQGRGVFAIPGSINNPLARGCHA"
BASE COUNT          537 a          910 c          832 g          525 t
ORIGIN      
        1 tctttttcga gcttgcgacg aatgcgctcg cgcttcagaa ccgacaggta gtccacgaac
       61 agcttgcccg ccaggtggtc gatctcgtgc tgcaaacaga ccgcgcccaa cccatccaga
      121 tcgcgttcga aggtctgccc gtccacgtcc tgcgctcgca ctctcacgca tgccgcacgc
      181 tcgacttgct caaacacatt cgggaccgac aagcagccct cctccgatac ctcaacccca
      241 tcgcgcgtca gaatttcggg attgatatag acctgaggcg cccggctgtc cccaccgatg
      301 tcgatcacga ctacctgccg gtggacattt acctgcgttg cagcaagacc gatacccggg
      361 gcggcgtaca tcgtttccag catgtcggcc acgagctgct tcaaccccgc atcaaactcc
      421 tcgactcgct tggcgcgggt gcgcagccgg ggatcgggga attccagtac gggcaacaat
      481 gccatccgcg ctatctccgt tcctaaactt taagaagtat tcttaaatgt tgggaaatat
      541 gccaaaattt caagaatcag agacgtatcg atccaccgcg tgatccagtg cacaaagtca
      601 gcaatcaacc gtgtgcgttt gcgccctctt aagtttataa cagctgaacg agaaggccct
      661 acccgacccc aagaggtagc agggatgtat ttgaatcatg tattgactgc cagacaggct
      721 cttaccgcct gcgtgagcct atccggggtc ctagccctcg gtggctgcag tatgttccat
      781 catgagactg cgcagtcggc tgtaccgccg cctgcgacgc aagtcgcaca gacggacacg
      841 actaacgcca gcgcgcccga actccaaacc gtcaccgacg cgctgagcga caccgatgag
      901 gcgggtacca ctaccgtcat gcgggatgcc gggcctgcac ttcgcgctgg cgccccgaag
      961 gattatgtcg tgaagcgtgg cgatacgctc tggggcatcg ccaacatgtt cctgaaggac
     1021 ccgtggctgt ggcccgagat ctggtacgtc aatccggaca tccacaatcc gcatcgcatt
     1081 tatcctggcg acaccgtcca cctggcactg ggcggcgacg gccgcactgc gctgcaggtg
     1141 gtgcgtggaa gcaccgtggg cggtacgcga cttgagccgc tgctgcgtag tagtccgctt
     1201 gaagggccga tcgcgacgat cccgtattct gcgatcgcgg cgttcctgtc acgtcccggc
     1261 gtgatgactc gcgaggaggt caaagccgcc ccttacgtgg tcgcgctgcg cgatgagcat
     1321 caggtcgcgg gcgcgggcca cgacctgtac gtgaaaaagc tctccggcga cgcgggcacg
     1381 cgctacaacg tgatgcacat cgatgagccg ctgaaggatc cgcagagtgg gcgtcatctg
     1441 ggctacatgg cgatctacac cggaaccgcg caactgacgc gcagcggacc gatcgcgcgc
     1501 gccacgctgg tggattctgc acgcgagacg ctgcagggcg atgtgctgat tgcgcaaacc
     1561 accaacccga cttccgattt tctgccgcac ccgccatcgc agccgatcga tgggcgcatc
     1621 attgcggtgg tcgacaacgt attgctcgcc ggtcaatacg agatcgcggc tctcaatcgc
     1681 ggctcggagg atggcctcga ccgcggcacc gtgctgacga tcgaggaggg gcaacgggtc
     1741 gctaccgatc attgcgctca catcgatggc agcagcacct gcttcgcggg ccactcgacg
     1801 cagctaccca ttgagaccga gggttcactg ctggtcttca agacctatga gaagatgagc
     1861 tacgcattga tccttagcag taccgttccg attcatatga acgacaaggt ccgcaacccc
     1921 tagtgcaatc aagcgtgcgc atagcgcaag cttgattttt ccggcgaata catcacgagt
     1981 cgatacgctt cgcgcgctgc gagcgttcta tgctgccgca tggacgcgat cgacttgcag
     2041 ctggcgctgg gccgcgcgcg cggcctgacc gcatcaaagc tgcgccgcta cggcgcgacg
     2101 ctgggtgagt gcgccgggca gcgcgggttg cccgccctgt tcgaacagtc gccgccggtg
     2161 cgcacggcgt taatggcagt cgacgcgacg ctcatcgccg ccgatcgccg ctggatccag
     2221 cgcgaatcga tcagcctgat tgatctgacc agccccggct atccctcgtt gctggcgcag
     2281 ctgcgtgacg ccccggcgtt gctgtacgtc aaaggtgatg tcgccgcact gtccacaccg
     2341 cagttggcga tcgcggggac tcgcaaggcc agcgctccgg cacaactcac cgcacgcaat
     2401 ttcgctgcgc gcctcgcccg tgaaggactg accgtcacca gcggtctggc actgggcatc
     2461 gacgctgcca gccacgaggg cgccctgcat gtcggcggcc ggactattgc ggttctcggc
     2521 acgggccttg atcagatcta tccggcaacg catcgcgtgc tcgcgcagcg catcgccgcg
     2581 caaggcgcgc tggtcagtga gttgccgccg ggtgtgcccc cattggcgtc aaactttccg
     2641 agacgcaatc ggatcatcag cggcctgagt cttggtgtgc tgatcgtgga agctgcgcgc
     2701 gatagcggct cgctcatcac agcggcactg gcgcgcgatc agggtcgagg agtctttgca
     2761 atccccggtt ctatcaacaa cccgctggcg cgcggatgcc atgc
//
LOCUS       DATMRM010000343         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_176565, whole genome shotgun sequence.
ACCESSION   DATMRM010000343 DATMRM010000000
VERSION     DATMRM010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176565"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="VNZ06_10175"
     CDS             <1..1102
                     /locus_tag="VNZ06_10175"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWW21160.1"
                     /translation="LGAEVLKIERPQSGDDFRDFARLPGWEVSPSFIAVNAGKRSITV
                     NLKAAEGREIIRRLVATADVLVENQRPNLLESLGLGWRELRQIKPDLIYCSLSGFGQT
                     GEMHSWPAYDHTVQAMSGMGWTGADDDIPSQGRGFSIDCFSGYVAFSSILSALFRRER
                     SGEGQFLDVAMLDSAMVLMAVGLVRQMLTGDRIKATQPIVHDRPTVGAFRTADGWIWI
                     SGNFQNHFLALCRVLGRSELAQDPRFSSSEARQQHADELKAELAGLIATRQAEELEIE
                     LMRAGAPAARVRTTQQALQIASLAERGMLQATQTPDGRPVTVFNSGFVADADSPALSG
                     PVPRIGAHTHELLAELGFDAGAIGAMKQKGII"
     gene            1117..2229
                     /locus_tag="VNZ06_10180"
     CDS             1117..2229
                     /locus_tag="VNZ06_10180"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HWW21161.1"
                     /translation="MSTALELLRAHYRDRLMAAQAVRASGASCVVGLTGPTAPVELVL
                     ACGGFPVRISPTEGIATPTAEHYLDAQLARDVQILFECAMHGDFEFLDLLVLTRGEDK
                     LFYFLKEMVRLGRAPAVPPMHMFDLMGSQRAAVREYNLQNLHWLMAAIERTSARTITD
                     EALSESIHLTNRCRQLQRMLLAQRWAGRLCGVEAMEVIGAGFCMHPIRYAQTLERYLR
                     ELEAAPVLKAAPRLMLASSEPLMNLQLHQRVEEAGGRVVAEDDPWGSRAPGEDIASAG
                     DPKQALLHKYWQDTAHAGVAPREARQAWFRLQIQRPDLDGVIFYVPPGDQQFGWDYPG
                     LAAQVKAAGKASLLLRQDAALGAAVPALIREFIACR"
     gene            2226..>2452
                     /locus_tag="VNZ06_10185"
     CDS             2226..>2452
                     /locus_tag="VNZ06_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21162.1"
                     /translation="MTDAERAASRARHEALTQLQVSRTLRTYQKAWFEKLRQEVFEQG
                     RPYAMGGALVPHEIWEALDLPFITDVWYSGLV"
BASE COUNT          412 a          749 c          856 g          435 t
ORIGIN      
        1 gctcggtgcg gaggtgctca agatcgaacg gccgcaaagt ggtgacgact ttcgcgattt
       61 tgcccgccta cccggctggg aggtcagtcc ctcgttcatc gcggtcaacg ccggtaagcg
      121 ctcgatcacg gtcaatctga aggccgccga agggcgcgag atcattcgcc ggctggtggc
      181 gacagcggat gttctggtcg agaatcagcg cccgaatctg ctcgagtcct tggggttggg
      241 ctggcgcgag ctgcgacaga tcaaacccga tctgatctat tgctcgctgt cgggctttgg
      301 ccagacgggc gagatgcata gctggcccgc gtacgatcac acggtgcagg cgatgtcggg
      361 catgggctgg accggcgccg acgacgacat cccctcgcag ggtcgcggct tcagtatcga
      421 ttgcttcagc ggctacgtgg ccttctcatc gatcctcagc gcgttgttcc ggcgtgagcg
      481 gagcggcgag ggccagttcc tcgacgtcgc gatgctcgat tccgcgatgg tgctgatggc
      541 agtgggtttg gtgcggcaaa tgctcacggg cgatcggatc aaggcaaccc agccgatcgt
      601 gcacgaccgg ccaaccgtcg gtgcatttcg cacggccgat gggtggatct ggatcagcgg
      661 caattttcag aatcactttc tggcgctgtg ccgggtgctc ggtcgcagcg agctggccca
      721 ggatccgcgt ttcagcagca gcgaggcccg tcagcagcac gccgatgaac tcaaggctga
      781 gctggccggg ctgatcgcca cccgtcaggc tgaggagctg gagatcgagc tgatgcgggc
      841 aggcgcgccg gcggcgcgcg tgagaaccac ccagcaggca ctgcagattg cttcgctggc
      901 cgagcgcggg atgctgcagg ctacccagac acccgatggc cggccggtca cggtgttcaa
      961 ctcgggcttt gtcgccgacg ccgacagtcc ggcgctctcg gggccggtgc cacgcattgg
     1021 agcacacacc cacgagctgc tggccgagct gggcttcgat gcgggtgcca tcggcgccat
     1081 gaagcaaaag ggcatcatct agaagctgca agcacgatga gcaccgctct ggaactgctg
     1141 cgggcgcact atcgggatcg tttgatggcg gcgcaggcgg tgcgcgcgag cggcgcatcc
     1201 tgcgtggtgg gtctcaccgg gcccaccgct ccggtcgaac tggtgctggc gtgcgggggc
     1261 tttccagtgc gcatcagccc gaccgagggc atcgcgacgc caacggccga gcactacctg
     1321 gacgcgcagc tcgcgcgcga cgtgcaaatc ctgttcgagt gcgcgatgca cggggatttc
     1381 gagttcctcg atctgctggt gctcactcgc ggcgaggaca agctgttcta ttttctgaag
     1441 gagatggtgc ggctcggacg agccccggcg gttccgccga tgcacatgtt cgatctgatg
     1501 ggcagccagc gcgccgcggt gcgcgaatac aatctgcaga atctacactg gctgatggca
     1561 gcgatcgagc gcacctcggc ccgcactatc accgacgagg cgttgagcga gtcgatacat
     1621 ctgaccaatc gctgccgcca attgcagcgc atgctgctgg cacagcgctg ggccggtcgc
     1681 ctgtgcggtg tggaggccat ggaggtgatc ggggcgggct tctgcatgca tccaatccgc
     1741 tacgcgcaga cgctcgaacg ctatctgcgc gaactcgagg ctgcaccggt gctcaaggcc
     1801 gctccacggc tgatgctggc cagctccgag ccgctgatga atttgcagct gcaccagcgt
     1861 gtggaagaag ccggcggacg tgtggtcgcc gaagacgatc catggggcag ccgcgcgcct
     1921 ggggaggata tcgccagtgc cggcgatccg aagcaagccc tgctgcacaa gtactggcag
     1981 gatacggcgc acgcgggtgt cgcgccgcgc gaggcgcgtc aggcgtggtt caggctgcag
     2041 atccagcgtc ccgacctgga cggcgtcatt ttctatgtgc cgcccggtga tcagcagttc
     2101 ggctgggact atccggggct cgccgcgcag gtgaaggcgg ccgggaaggc gtcgctgctg
     2161 ctgcggcagg atgccgcgct cggggccgcg gtgccagcac tcatccgcga attcatcgca
     2221 tgtcgatgac tgacgccgaa cgcgctgcct cgcgcgcgcg ccacgaggcg ctgactcagc
     2281 tgcaggtgtc gcgcacgctg cgaacctatc agaaggcatg gttcgagaag ctgcggcagg
     2341 aggtgttcga acagggtcgg ccctatgcga tgggcggcgc gctggtacca cacgagatct
     2401 gggaggcact ggatctgccg ttcattactg acgtctggta ctcgggcctg gt
//
LOCUS       DATMRM010000344         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_824833, whole genome shotgun sequence.
ACCESSION   DATMRM010000344 DATMRM010000000
VERSION     DATMRM010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824833"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VNZ06_10190"
     CDS             complement(<1..234)
                     /locus_tag="VNZ06_10190"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWW21163.1"
                     /translation="MAKNVICLWYNGTAEDAARFYAQTFPNSAVTAVHRAPGDYPAGK
                     QGQVLTVEFTVLGIPCLGLNGGPAFKHSEAFSFQ"
     gene            complement(377..994)
                     /locus_tag="VNZ06_10195"
     CDS             complement(377..994)
                     /locus_tag="VNZ06_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HWW21164.1"
                     /translation="MPENARSLLSPLSPLKPLSERLLFASNFLRHPNMLGSIIPSSRF
                     LVDQVLEPIDWGRTRVIVEYGPGIGTFTGEILRRMSSDAQLVAIETNRDFVRFLQRAL
                     PDPRLHVVHDSAAEVQPVLMRLALPAPRYIISGIPLGSMPDPVRTDIAVKSRAALEPG
                     GAFLVYQFTARALPTLQRTFADVRRRFERRNFPPAQLFLCTTQGA"
     gene            complement(1034..1864)
                     /locus_tag="VNZ06_10200"
     CDS             complement(1034..1864)
                     /locus_tag="VNZ06_10200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWW21165.1"
                     /translation="MSELAVITGTTHGIGRVTSRELARAGKAVVMLCRDVTAADETRD
                     EIMRHAPRARVEVVRCDLASLASVREAAAAVRRDYPPLGLLVNNAGMVSARHRTSVDG
                     FELTFATNHLGPFLLTALLSDHLDKVARIVMVASRIHYRGRLDLATVTDARARYGATA
                     AYARSKLANVMHTFALARRMAGTGISVNCLHPGVVATNLLPRWLRIIKPLFTRVVFDA
                     ERGARTTVHLALDQSVAGVSGRYFDEYQLPRSASALANDVELQESLWNMSAVWTGAPQ
                     "
     gene            complement(1861..>2222)
                     /locus_tag="VNZ06_10205"
     CDS             complement(1861..>2222)
                     /locus_tag="VNZ06_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HWW21166.1"
                     /translation="VNPIALRRIENLFCKNERAIIRCRLSASDYLITLVPVAAILVAS
                     IRLHCADVLLHLKYRGPNTIPCDAELRKGEEMGWFQHGSTMIAFAPPGMVLASGLGQG
                     SVIRMGEPLLSIAGRVS"
BASE COUNT          411 a          681 c          749 g          381 t
ORIGIN      
        1 ctggaacgag aaggcttcgc tgtgtttgaa agcggggccg ccgttcaggc ccagacaggg
       61 tattcccagc accgtgaact cgaccgtcag cacctgtccc tgcttgccag cagggtaatc
      121 cccgggcgcg cgatggacag cggtgacggc gctgtttggg aaggtctggg cgtaaaacct
      181 ggcggcatct tctgcagtgc cgttgtacca aaggcagatc acgttcttag ccatcgtctg
      241 tcgctcctca ttcgtcagtc ggtgcactcg tcctagtatg aatcgacggc ggacctgagg
      301 cgtatttacg gtcgcggtcg cggtcccgct ccatcagctg agcaaggtga cgtcgtttcg
      361 gccggcacac cggccttcag gccccctgtg tcgtgcatag gaacagctgc gctggcggga
      421 agttccgtcg ctcgaagcgg cgtcgcacat cagcgaaggt ccgctgcagc gtaggcagag
      481 ctcgggcagt gaactggtag accagaaacg ctccgcccgg ctcgagcgcc gcccggctct
      541 tcaccgcgat gtcggtgcgc accggatcgg gcatgctgcc gagtgggatg ccggaaatga
      601 tgtatcgcgg cgccgggagt gcgagccgca tcagaaccgg ctgcacctct gcggcggagt
      661 cgtgcacgac gtgcagccgc ggatccggta aggccctttg caggaaacgc acgaagtcac
      721 gattcgtctc gatggccacg agctgcgcat cgctgctcat gcgccgcagg atctccccgg
      781 tgaacgtgcc gatacccgga ccgtattcca cgatcacacg ggtacggccc cagtcgatag
      841 gctccagcac ctggtccacg aggaagcgcg agctcgggat gatcgaaccg agcatgtttg
      901 gatgacgcag aaagttgctc gcaaacagca gcctttcgct gagcggcttg aggggcgata
      961 gcggcgatag tagcgatcgg gcattttccg gcatgacgag cctctcagtc aggatggtaa
     1021 cgtgcgttat cggtcactgt ggcgctccgg tccacacggc gctcatgttc cacagcgact
     1081 cctgcagctc aacgtcgttc gcgagcgcgg aagcggaacg aggaagctgg tactcatcga
     1141 aatagcgacc gctgactccg gcgacactct gatcgagcgc aaggtggacg gtggtgcgcg
     1201 cgccgcgctc ggcgtcgaac accacccgag tgaacagcgg cttgatgata cgcagccatc
     1261 gcggcagaag attcgtggcc acgacgcctg gatgcaggca gttgacgctg atgccggtac
     1321 cggccatgcg gcgcgcgagc gcgaatgtat gcataacgtt ggcgagcttc gagcgggcat
     1381 aggcggcggt ggccccgtaa cgggcgcgcg cgtctgtgac cgtggcaaga tccaggcggc
     1441 cgcggtagtg gatccgggag gcgaccatga cgatgcgcgc caccttgtcc aggtgatccg
     1501 acaacagggc ggtgagcagg aagggaccca ggtggttggt cgcgaacgtg agctcgaagc
     1561 catcgaccga ggtgcgatgt cgagcgctca ccatgccagc gttgttgacc aggagcccga
     1621 gcggcgggta gtcgcggcgg acagcagccg cggcctcgcg cacggaagcg agcgaagcga
     1681 gatcgcagcg gaccacctcg acccgggcgc gaggcgcgtg ccgcatgatc tcatcgcgcg
     1741 tctcatctgc ggctgtcacg tcgcggcaca gcatgacgac cgctttcccc gcgcgcgcga
     1801 gctcacggct tgtcacgcgg ccgataccat gggtagtgcc ggtgatgact gcgagctcgc
     1861 tcatgacact ctgcccgcaa tactcagcag cggctcgccc atgcgaatca cgcttccctg
     1921 gcccagtccg gaagcgagca ccatgcccgg gggtgcgaag gcgatcatgg tggagccgtg
     1981 ctggaaccag cccatttcct cgcctttgcg cagctcggca tcgcagggaa tggtgttagg
     2041 tccgcggtac ttcaggtgca gcagcacgtc cgcgcagtgc agccggatgc tcgccaccag
     2101 gatggcggcg accggcacca gcgtgatgag gtagtccgat gcgctcagcc ggcagcgaat
     2161 aatggcgcgt tcgttcttgc agaagagatt ctcgatacgt cggagcgcga tcggattgac
     2221 gt
//
LOCUS       DATMRM010000345        17186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1440285, whole genome shotgun sequence.
ACCESSION   DATMRM010000345 DATMRM010000000
VERSION     DATMRM010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17186
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440285"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VNZ06_10210"
     CDS             complement(<1..550)
                     /locus_tag="VNZ06_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21167.1"
                     /translation="MDRLFPSWLARVERHERRAVVLACLCNLVLYASYYILRPVRDTM
                     ATVFGSGELQNLFTGTFIATMIASPIFAVIASRVKIRHLVPGVFWFLILGILLFDLVL
                     KDRTDNLWIAGSYYVWFSTINLFLVSVFWSLMVDTFTAAQATRLFGFIAFGQEIGAIL
                     GPVLTRTLVKYLQLGGLLLLAAA"
     gene            complement(552..1619)
                     /gene="lptG"
                     /locus_tag="VNZ06_10215"
     CDS             complement(552..1619)
                     /gene="lptG"
                     /locus_tag="VNZ06_10215"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HWW21168.1"
                     /translation="MTLLDRYIARTLLSAVALVMAALLMLGMLFVFIGEQGSVGVGHY
                     SIFDALLYAAMSLPQFAVESFPAGALIGALVGIGILARSNELTVMRASGMSKLRLSRS
                     VLISSAVLIGIAVLVGEFLAQPLAQLADAQKAFAKYSNVSFATAGGAWVRDGDTILNV
                     QGRSSAAEFGGMLIFELTEDNRLLAVGRADHANATGPQSWQLRDYYESRITDGNVTSV
                     HAPVRELRTTAGADFLQLAVTDPAELALHILYPAIRYLRANHLDAKQYEIAFWSRIAS
                     TLGILAAMLFAMPFGLGSLRSTSLSARTTFGVALGVVYIFAQRLVESGTQVFNLDPVL
                     LSGVPTALLALAAGVMIWRVR"
     gene            complement(1616..2701)
                     /gene="lptF"
                     /locus_tag="VNZ06_10220"
     CDS             complement(1616..2701)
                     /gene="lptF"
                     /locus_tag="VNZ06_10220"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HWW21169.1"
                     /translation="MRILQRYMLQEMTANFLGVTLVALALLLVNQVGSVLARAAQLQY
                     QKGFVLELIGLGTAQNVAILLPFGLMLGIVLAFGRLYHDSEMAAAQACGVGVGRVAVA
                     VWLLALPVTALIAWLNFDLAPRAASAEANMRSEALRAAAAAPLEPGTFRSFSGGRIVI
                     YARAAEKNGDLTDVFVKRSEGLAVETTVARRAHYQVSSDGESETITLFDGERVEGTPG
                     NNRYRIMQFDQQLIPVRTPETAARAQRLDEMPTLSLIGSRDPKLTAELQWRVGLPLMT
                     LVLTLLAVPLGRLRPRQGRYAHVWIAVLVFALYANLALAGRTWLSRAVIPQALGLWWV
                     HGLFLLASMGAMAGPSLVRLQRVRRQA"
     gene            2833..4329
                     /locus_tag="VNZ06_10225"
     CDS             2833..4329
                     /locus_tag="VNZ06_10225"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWW21170.1"
                     /translation="MRFETFSRRAADQRSDCLVIGAFEQGELGTEAAAVDRAMRGRVK
                     TLLSRGDFSGRSGETLLLPEVSGVRSQRLLLVGLGPKAQFNRKAWRRGMNQAITALAR
                     TRISSAAIALERPAARELDDYYFGRASAEITGSTLYRINDLKTARRAPLPRLGTVFFG
                     PVSTSATADVRLGLRNGAALSDSGELLRNLGNLPPNVCTPRYLGARARELQSRYRQRV
                     RVRVFDEAAIKRLKMGCFLAVTRGSEEPPRFIVLEYRGGRRGRAPVVLVGKGVTFDTG
                     GISLKDPPAMDEMKYDMCGAATVLAAIDFAARSKLRLNVVGLVPACENMPDGRAIKPG
                     DIVTSASGQTVEILNTDAEGRLLLCDALNYARRYSPDVVVDFATLTGACLVALGNHHS
                     GVMANDDALARELVDAGVRSDDRAWQLPLTEEYAEQLRSNFADMANMAGREGGAITAA
                     AFLGKFTQGMRWAHMDIAGTAWLSGAAKGATGRPLALVSDFLTRRAAG"
     gene            4329..4778
                     /locus_tag="VNZ06_10230"
     CDS             4329..4778
                     /locus_tag="VNZ06_10230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HWW21171.1"
                     /translation="MPQVAFYVLPSSDDRARLKFACRLTERAYLAGEQVFVALTDAAQ
                     MQQFDELLWTFADRSFVPHEPYRDDQQWHETPVLLGCGPTQPQQAFDLLVNLSDEIPA
                     ALAHAQRVSEIVDADESRRRAGRQRYRGYRDQGVIPETHNIAADQGS"
     gene            4814..7585
                     /locus_tag="VNZ06_10235"
     CDS             4814..7585
                     /locus_tag="VNZ06_10235"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWW21172.1"
                     /translation="MDSKYSPAEIEQRLYQRWEESGYFAPRPSSRHFCIVIPPPNVTG
                     TLHMGHAFQHTIMDALTRWHRMRGDCTLWQPGLDHAGISTQMVVERQLNAQGRKRTEL
                     SRGEFVERVWAWKNESGGIISGQMRRLGASVDWSRERFTMDEGLSRGVVEAFVRLHDE
                     GLIYRGKRLVNWDPKLLTALSDLEVLSEEEAGHLWYLRYPLADDSGHVIVATTRPETL
                     LGDVAVAVNPQDERYRSLIGRLVRLPLAKRDIPIIADDYVDPQFGTGCLKITPAHDFN
                     DYAIGQRHGLPLINVFTPDAKLNDQAPAHLRGLDRFEARKRIVDELQAAGLVDRIEPH
                     KLMVPRGDRSGEVLEPYLTDQWYVRIAPLATPAIKAVESARVRFVPENYDRTFFQWMH
                     HIQDWCISRQLWWGHRIPAWYDDQGTVYVARTEAQALQQARAKRGQDVELRQDEDVLD
                     TWFSSGLWPFSTLGWPDSTPELRRFYPTDVLVTGFDIIFFWVARMLMFGLKFADDVPF
                     RDVYITGLILDHEGQKMSKSKGNILDPLDVVDGIGLEALVAKRTTGLMQPHLKSRIEK
                     TTRKEYPQGIEAYGTDSLRFTLASLATQSRDLRFDLNRVAGNRNFCNKLWNAARFVML
                     SSESCPAGPSSAADFSVADRWIRSRLGETIRTVERAFSEYRFDFAASALYEFTWHEFC
                     DWYLEIAKPVLQNSADARAQASARGNLLTVLESLLRTLHPLMPFITEEIWMRVAPMAG
                     VGGPSIMLSPWPKADEFAPDPDADEEMHWVMQVVLGIRQIRGVLDIPASRKLPLLLHN
                     ASARDLQWLAKHRVLLMRLAGLESMQPLAHAEAAPPAAAELVGELVLLVPMAGLIDPA
                     SELERLEKRLARVEAELKSFRGKLANENFVRNAPPEVVAQEHERVSDRERARAGLIRQ
                     IEQVRKLLN"
     gene            7588..8499
                     /locus_tag="VNZ06_10240"
     CDS             7588..8499
                     /locus_tag="VNZ06_10240"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWW21173.1"
                     /translation="MSRISDTVCAVERVILGKSLQVRLCLTCILARGHLLIEDVPGVG
                     KTTLAHTLAHVLGLEWTRVQFTSDMLPADILGVSIFDRSTHKFQFRRGPIFTQLLLAD
                     EINRASPKAQSALLEAMEERQVTVDGETYPLPEPFFVVATQNPHEQIGTYGLPESQLD
                     RFLMRVSLGYPDRASERRVLLQGERKEILERTPSVLNGEQVLAFQRAARSMHVADALL
                     DYVQSLIARSRTQMSLGLSPRAAQGLLRAAQAWALLGGEKAVLPEHVQAVFPAIAAHR
                     LEPRESDGSSDGNQIADGLIKAVPVPV"
     gene            8465..9454
                     /locus_tag="VNZ06_10245"
     CDS             8465..9454
                     /locus_tag="VNZ06_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWW21174.1"
                     /translation="MDSSKPSRFRFDARGRLAARAQRWAQARQGDDSPPLTLLQRRIY
                     ILPTKAGVSTSVLLFAMLLAGMNYGNSLALFLCFTLSGVALVSMHECHRSLAGLKLLR
                     AHVENSFAGQVGELQLYFENVDTRLRDSLSVKCSPCDEVAFRVQPGEIKPVTVNFLAR
                     TRGRQRIDRLRLSTTAPMGLFRAWAWIHLPLEAIIYPAATGNRALPPRQGEPRLGRQL
                     THNTGEEEWAWLRNYQNSDPPRSVAWKAYARGAPLMVAHYDSPAGMQRTLRFSAVPGN
                     SVEQKLSQLTRWVLECERLGEDYALELPEATQASGHGVAHRRACLESLAQYGL"
     gene            9466..11430
                     /locus_tag="VNZ06_10250"
     CDS             9466..11430
                     /locus_tag="VNZ06_10250"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HWW21175.1"
                     /translation="MPARTVWLLLGGYLGAVLLNVHHTALWCLPLALGVAIWRARRLR
                     TSAGKSRRLARTVVIVILTAIVLIGFRTLNGVQAGASLLVAMAALKLTETSARRDWLI
                     MVGASLFLLLAACLDAQMLWRLPLYVAELCLLCMGMYALGAGDQVPSLAPLTHRTAGT
                     LLAAMPLAILLFLFVPRVAGSFWALPRSHEATTGLSDEMNPGNISSLSVSGDPAARVR
                     FDGPPPPPEQRYWRGFVLDKFDGNTWRRSRRDYGFAPPLTYLGTSYQYEVSLEASQLT
                     VLLALELPQGAPESLGDAHLSDDFQVIDSQPVTQRLTYRMQSYPLHRSLPQQQLTPEE
                     RQRYLGFPGARNLRSIELAHTLRASADSDLGYIRSVLDYFARGGFKYALDPELSNGNS
                     VDDLLFNTHEGFCGHYASAFAQLMRAARIPARVVTGYQGGIWNRYGNYLLIRQSDAHA
                     WAEVWLDASGWVRIDPTAVVAPNRLTRGSDDLTSNATLAARMFRWQWLANTVQAWQAV
                     NAWWQDEFVGFNFSKQQDLLGLLGIQRHYLRALAVMLAIGGSIWLGVIAWSLRPRHSR
                     RAEDVLGRTWRLLERKLQRAASPRASYEGPVSYAQRIGLERPELASRVSALARRYAWL
                     RYGPKPSNEEVEQFRRAVRLLKTMPARITR"
     gene            complement(11427..12671)
                     /locus_tag="VNZ06_10255"
     CDS             complement(11427..12671)
                     /locus_tag="VNZ06_10255"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW21176.1"
                     /translation="MTSKHLAQWSWSLSACANHGFITTIAVGFFPIFFDKYVATSLSG
                     TQQTFYLGLANSTATFIVMLLAPWLGALADRRGHKKRWLGIWTVLGSIGSAVLATVGQ
                     GEWVWALVVYGVGTVAFWAGSSFQDALIVQVADASEINRVSAQGYAFGYIGGGLLFLV
                     NVLMALHPSWFHLANGTQATRIAFLDVAVWWLLFTLPLFRHVPEGRPTGEPSGVAELW
                     ATIRKVMRERMVRNFLLAYWLYIDALNTVQLMAVDFGSKLGFPSDSLIVALLIVQFIA
                     FPFALLFGWLGDRFGTKGSIYLGLAVFVGVTFYAYFMQTVQQFYILAAIFGSVQGGVQ
                     ALSRSYFARLIPADRAGEYFGFYNMLGKFAAVLGPLAMGLVAMVTGNQRLSILVLEFF
                     FIGGGLLLARVRAPERSVCAMA"
     gene            complement(12700..14289)
                     /locus_tag="VNZ06_10260"
     CDS             complement(12700..14289)
                     /locus_tag="VNZ06_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HWW21177.1"
                     /translation="MTTLEQEIARRRTFAIISHPDAGKTTLTEKLLLFGGAIAMAGTV
                     KGRKAARHATSDWMRLEQQRGISVTSSVMQFPYRDHVINLLDTPGHEDFSEDTYRTLT
                     AVDSALMVIDCAKGVEERTIKLMDVCRLRDTPIMTFINKLDRDGRAPIELLDEIERVL
                     KIQCAPVTWPVGMGRELKGIYHLPEDRIYVYEAGERGRVGGNRVIDGLHSPAAAELFG
                     DSAQAFADEIELVRGATAAFDAQAYRAARQTPVFFGSAISNFGVEELLRSFVTHAPAP
                     LPRPSRERSVSAQEPKLSGFVFKIQANMDPGHRDRIAFLRLCSGRYQRGMRLKHVRLD
                     KEMRIADALTFMAADRSQAEEAYAGDIVGLHNHGTINIGDSFSEGESLHFTGIPNFAP
                     EMFRRAVLKDPLRMKALSKGLDQLCEEGATQLFRPLRNSELILGAVGQLQFEVVTFRL
                     QDEYNVQCVFESVTVHTARWVTAADEGKLEEFRTRAYEHLALDHGGALVYLAPSRVNL
                     QLTRERWPEIQFHETREYLMQ"
     gene            complement(14306..14755)
                     /gene="rimI"
                     /locus_tag="VNZ06_10265"
     CDS             complement(14306..14755)
                     /gene="rimI"
                     /locus_tag="VNZ06_10265"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HWW21178.1"
                     /translation="MPELSFRSARESDVLEILRIERLSYAFPWTEGIFRDCLRVNYTC
                     RVAEIGVAVAGYGIMSVAAGEAHLLNLCVGEDYRCRGIGRRLLAHLLQVAATVGAREA
                     FLEARPSNTSAIRLYQSLGFVQIGVRRGYYQAVDGREDAIVLKRHID"
     gene            complement(14794..15447)
                     /locus_tag="VNZ06_10270"
     CDS             complement(14794..15447)
                     /locus_tag="VNZ06_10270"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWW21179.1"
                     /translation="MRAREQYLQALGLDVWVPRFATAPAPPQAGAVWDELQARVRECT
                     RCGLCQTRTQTVFGVGQRRAELMVIGEAPGQDEDEQGEPFVGRAGQLLNSMLQAMGQP
                     RERVYIANILKCRPPNNRNPNPTEVASCLPYLQQQIDLIQPRMILAVGGIAAQNLLAT
                     DVSVGRLRGQVHRLGERATPLIVTYHPAYLLRSPGEKRKVWTDLKFARTELARARGN"
     gene            complement(15454..16236)
                     /gene="pssA"
                     /locus_tag="VNZ06_10275"
     CDS             complement(15454..16236)
                     /gene="pssA"
                     /locus_tag="VNZ06_10275"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HWW21180.1"
                     /translation="MDPESPPARQPARGIYLLPNLLTTGCLFSGFYAIVAAIDGNFER
                     AGVAVFIAMIFDGLDGRVARLTRTESAFGKEYDSLADMVAFGMAPAIVSYQWGVARIA
                     EYGKEWFRFGWLAAFFFAAAAAMRLAKFNSRVQDKRYFEGLPSPSAAAVVAAFLWTSS
                     EWWREPGLPGLILAFGITATAGALMVSRFPYSSGKGLDWTRPVSFVWLVVIAAIFVVI
                     GGAREIVLLVFFGSYAAYSPLMWVWRRLRRGKRPVRAVTPEA"
     gene            16303..>17186
                     /gene="proS"
                     /locus_tag="VNZ06_10280"
     CDS             16303..>17186
                     /gene="proS"
                     /locus_tag="VNZ06_10280"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWW21181.1"
                     /translation="MRLSQSPIATVKETPSEAEIVSHQLMLRAGIIRRLAAGIYTWLP
                     LGLRTLRKVENIIRQEMDRAGALEMLMPAVQPAELWQESGRWIKYGPELLRLKDRHQR
                     DFVVGPTHEEVITDIARRELKSYRQLPVNFYQIQTKFRDEVRPRFGVMRGREFIMKDA
                     YSFHVNQASLNEGYAAMSAAYSRMFKRMGLNFRAVRADSGAIGGNASEEFQVLAESGE
                     DAIAVSDGDDFAANIELAKALPPAGPRPAPGKPMTRVATPGAKTIEQVSELLQVEPAQ
                     CLKTLIVAGSEGTAVVLL"
BASE COUNT         3314 a         5569 c         5328 g         2975 t
ORIGIN      
        1 ctgcggctgc cagcagcagc agacccccca gctgcaggta cttcaccaac gttcgagtca
       61 gcaccggacc caggatcgct ccgatctcct ggccaaaggc gatgaaaccg aacagccggg
      121 tcgcctgagc cgcggtgaac gtgtccacca tcaggctcca gaacaccgag acgaggaaca
      181 ggttgatggt actgaaccac acatagtagc taccggcaat ccacaggttg tcggtccggt
      241 ccttgagcac aaggtcgaac agcaggatgc ctaggatcag gaaccagaac acgcccggca
      301 ccagatgcct gattttgacg cgcgaggcga tcaccgcaaa aatcggtgag gcaatcatcg
      361 tggcgatgaa cgtcccggtg aacagattct gcaactcgcc cgagccgaac accgtcgcca
      421 tcgtgtcgcg caccggacgc aggatgtagt aactggcgta cagcaccaga ttacacaggc
      481 aggccagcac gacggcgcga cgctcatgtc gctcgacccg cgccagccag ctcggaaaca
      541 gacgatccac gctatcgcac tcgccagatc atgacgccgg ccgcgagcgc cagcagcgcc
      601 gtgggtacac cggacagcag caccgggtcg aggttgaaga cctgggtgcc gctttcgacc
      661 agccgctgcg cgaagatata gaccacgccc agcgccaccc caaatgtggt gcgcgcgctc
      721 aatgaggtcg agcgcagcga gccgagaccg aacggcatcg cgaacagcat ggcggccaga
      781 ataccgagcg tgctggcgat gcgcgaccag aaggcaatct catactgctt cgcatcgagg
      841 tggttcgctc gcaggtagcg gatcgcagga taaaggatat gcagcgccag ctcggccgga
      901 tcggtcaccg caagctgcag aaagtcggcg ccagccgtgg tgcgcagctc tcgcaccggt
      961 gcatgcacac tggtgacgtt gccgtcggtg atgcgcgact cgtagtagtc gcgtagctgc
     1021 caggactgcg gtccggtggc gttggcatga tctgcccggc ccaccgccag caaccggttg
     1081 tcctcggtga gttcaaagat cagcatgccg ccgaactcag cggcactcga gcgtccctgc
     1141 acattcaata tggtatcgcc gtcgcgcacc caggcaccgc cggccgtggc gaagctgacg
     1201 ttcgagtatt tggcgaacgc cttctgtgca tccgcgagct gtgccagcgg ctgcgccaga
     1261 aactcaccca ccagcacggc gatgccgatc agcaccgcgg agctgatcag caccgacctg
     1321 ctcagacgca gcttcgacat gcccgatgca cgcatcacgg tcagctcgtt gctgcgcgcc
     1381 aggatgccga ttcccaccag cgccccgatc agcgcgcccg cagggaatga ctccactgca
     1441 aactgcggca gactcatcgc ggcgtacagc agcgcgtcaa atatgctgta gtgaccaacg
     1501 ccgacgctgc cttgttcgcc gatgaacaca aacagcatcc cgagcatcag caacgcggcc
     1561 atcaccagcg ccaccgcgga cagcagagtg cgggcgatgt aacgatcgag cagtgtcacg
     1621 cctggcgtct cacccgctgc agtcgcacca gcgacggccc ggccatcgcg cccatactgg
     1681 cgagcaggaa caagccgtgt acccaccaca agcccagtgc ctgcggtatc actgcgcggc
     1741 tcaaccaggt gcgaccggcg agggccagat tggcgtacag cgcgaacacc agcacggcga
     1801 tccagacgtg tgcgtagcga ccctgacgtg gacgcagccg tcccagtggc accgccagca
     1861 gcgtcagcac cagcgtcatc agcggcagac cgactcgcca ctgcagctcg gcggtcagtt
     1921 tcggatcgcg gctgccgatc agcgacagcg tcggcatttc atccagtcgc tgggctcgcg
     1981 ccgcggtttc gggcgtgcgc accgggatca actgttggtc gaattgcatg atccggtagc
     2041 ggttgttgcc aggggtcccc tcgacacgct cgccgtcgaa cagcgtgatc gtctcgctct
     2101 cgccatcgct cgagacctgg tagtgcgcgc ggcgcgccac ggtggtctcc accgccagtc
     2161 cttcgctgcg cttgacgaac acatcggtca gatcgccgtt cttttcagcg gcccgagcgt
     2221 agatcacgat acgcccgcca ctgaaactgc gaaaagtccc cggttccagc ggcgcggcgg
     2281 ccgcggcccg cagggcttca ctgcgcatgt tcgcctcggc gctggccgcg cgcggggcca
     2341 gatcgaagtt gagccaggcg atcagcgcgg tgaccggcaa cgccagcagc caaaccgcca
     2401 cagcgacccg gccaacgccg acgccgcagg cctgcgcggc ggccatctcg ctgtcgtgat
     2461 agagacgccc aaacgccaaa acaataccga gcatcagccc gaaaggcagc aggatggcga
     2521 cattctgagc ggttcccaga ccgatcagtt ccaggacgaa acccttctgg tattgcagct
     2581 gggccgctcg cgccagcacc gagccgacct ggttaaccaa caacaaggcg agcgcaacca
     2641 acgtcacacc aaggaagttg gcggtcattt cctgaagcat gtagcgctgc aaaattcgca
     2701 acgggtccgc accctccgtg cgccttctcg gctaaactaa aacctgcacc aagttgccgc
     2761 ggcgactggg aggtcgctcg ccgcggtggc tacgttatac ggataattcg cgggcaaaca
     2821 aggattaaac aaatgcggtt tgaaactttc agtcgcaggg ccgccgatca gcgaagcgat
     2881 tgcctggtca ttggcgcatt tgagcagggc gaactgggaa ccgaggccgc tgccgtggac
     2941 cgcgcgatgc gcggccgggt caagacgctg ttgtcgcgcg gggatttttc cggccgcagc
     3001 ggcgagaccc tattgctgcc ggaagtgagc ggcgtgcgca gccaacggct gctgctggtg
     3061 ggtctgggtc ccaaggcgca gttcaaccgc aaagcctggc gccgggggat gaatcaggca
     3121 atcaccgccc tggcccgcac gcgcatcagc agtgcggcga tcgcactcga acgtcccgcc
     3181 gcgcgcgaac tggatgacta ctatttcggc cgcgccagcg ctgaaatcac cggttcgacg
     3241 ctgtatcgca tcaacgatct caagaccgcc cggcgtgcgc cgctaccgcg cctgggcaca
     3301 gtgtttttcg gacccgtgag cacttcggcc accgcggacg tgcgcctcgg gctgcgcaac
     3361 ggtgcagccc tgagcgacag cggcgagctg ctgcgcaatc tgggcaatct gccgccgaat
     3421 gtctgcacac cgcgttacct cggcgcccgg gcccgcgagc tgcaaagccg ctaccgccaa
     3481 cgggtgcgcg tgcgcgtttt cgacgaggca gcgatcaagc gcctgaagat gggctgcttt
     3541 ctggcagtga cccgtggcag cgaagaaccc ccgcgcttca tcgtgctcga atatcgcggt
     3601 ggccgccggg gccgcgcgcc cgtcgtgctg gtgggcaaag gggtgacgtt cgacaccggc
     3661 ggtatctcgc tcaaggaccc gccagcgatg gacgagatga aatacgacat gtgcggtgcc
     3721 gccaccgtgc tggcggccat cgactttgca gcccgctcca aactcagact gaatgtcgtc
     3781 gggctggtgc cagcctgcga aaacatgccc gacggccggg ccatcaaacc cggggacatc
     3841 gtcaccagtg cttccggtca gacggtcgag attctcaaca ccgatgcgga aggtcggcta
     3901 ctcctgtgcg acgcgctcaa ctacgcgcgc cggtactctc cggacgtggt ggtggatttt
     3961 gcgacgctga ccggcgcctg cctggtggcg ctgggcaatc accacagcgg tgtgatggcc
     4021 aacgatgatg cgctggcgcg tgaactggtg gacgccggcg tacgcagcga tgatcgcgcc
     4081 tggcaactgc cgctgacgga agagtacgca gaacagctgc gcagcaattt cgccgacatg
     4141 gcgaacatgg ctggacgtga aggtggtgcc atcaccgccg cagcattcct cggcaagttc
     4201 acgcagggta tgcgctgggc tcacatggac atcgcgggta ctgcctggct gagcggcgct
     4261 gccaagggtg ccaccggacg gcccttggcg ctggtgagtg atttcctgac gcgccgcgcc
     4321 gccggctgat gccgcaggtt gctttctatg tactgcccag cagcgatgat cgggcgcgcc
     4381 tgaagtttgc ctgccgcctg acggaacgcg cctatctggc cggagagcag gtgttcgtcg
     4441 cgctgacgga cgcggcgcaa atgcagcagt tcgatgaact gttgtggacc tttgccgacc
     4501 gcagcttcgt tcctcacgaa ccctatcgag acgatcagca atggcacgag acgccggtac
     4561 ttctgggttg cggcccgacg cagccgcagc aagcattcga tctgctggtg aacctgagcg
     4621 atgagattcc agccgcgctc gcgcacgccc agcgtgtcag cgaaattgtg gacgccgatg
     4681 agtcgcgccg acgcgcgggc cggcaacgat atcgcggcta tcgtgatcag ggtgtgatcc
     4741 cggaaacgca caacatcgcg gccgatcagg ggtcataggc cctataattg cggttttccc
     4801 aagaccgcct gtgatggaca gcaagtactc acccgctgag atcgaacagc gtctgtatca
     4861 gcgttgggag gaatcggggt atttcgcccc gcgcccctcc agtcgccatt tctgtatcgt
     4921 catcccgcca cccaacgtga ccggaacgct gcacatgggc catgcgttcc agcacaccat
     4981 catggacgca ctgacgcgct ggcaccgcat gcgcggcgac tgcacgttgt ggcagccggg
     5041 gctggatcat gccggtatct ccacgcagat ggtcgtcgag cgccagctca atgcgcaggg
     5101 acgcaagcgc accgagctgt ctcgaggcga attcgtcgaa cgagtctggg cgtggaaaaa
     5161 cgagtctggt ggaataattt cagggcagat gcgacgcctg ggtgcatccg tggactggtc
     5221 gcgcgagcgc ttcacgatgg acgaaggcct gtcgcgcggc gtggtggagg ccttcgtgcg
     5281 cttgcacgac gaggggctca tctatcgcgg caagcgcctg gtcaactggg accccaagct
     5341 gctgacggca ctgtcggatc tggaggtgtt gtcggaagag gaagccggac atctgtggta
     5401 cctgcgctat ccgctggcgg acgactccgg ccacgtcatc gtggcaacca cgcgcccgga
     5461 aaccctgctc ggtgacgtcg cagtcgccgt gaatccgcag gatgagcgtt atcgctcgct
     5521 gatcggacgc ttggtgcgtc tgccattggc caagcgcgat attccgatca ttgccgacga
     5581 ctacgtggac ccgcagttcg gcacgggctg cctgaagatc acaccggcgc acgatttcaa
     5641 cgactacgcg atcggccagc gccatggcct gcccctcatc aatgtgttca cgcccgatgc
     5701 caagctcaac gatcaggcac ccgcgcattt acgcggcctc gatcgtttcg aagcgcgcaa
     5761 gcggatcgtg gacgaacttc aggctgcggg tctggtcgat cgtatcgagc cgcacaagct
     5821 gatggtgccg cgcggcgacc gatccggcga agtgctcgag ccctacctga ccgaccaatg
     5881 gtatgtgcgt attgcgccgc tggccacgcc cgcgatcaag gcggtcgaat ccgctcgcgt
     5941 gcgcttcgtg ccggagaact atgacaggac cttctttcaa tggatgcacc acattcagga
     6001 ctggtgcatc agccgccagc tttggtgggg tcatcgtatt ccggcatggt acgacgatca
     6061 gggcaccgtc tacgtcgccc gtaccgaagc gcaggcgctg caacaagcgc gcgccaagcg
     6121 tggccaggac gtcgagttgc gacaggacga ggacgtgctg gatacctggt tttcatccgg
     6181 cctgtggccg ttctcgacgc tcggctggcc ggacagcacg cccgagttgc gacgcttcta
     6241 tccgaccgat gtgctggtca ccggcttcga catcatcttc ttctgggttg cccggatgct
     6301 gatgttcgga ctgaagttcg ccgacgatgt cccgtttcgc gatgtctaca tcaccggact
     6361 gatccttgat cacgagggtc agaagatgtc caagtccaag ggcaatattc tcgacccgct
     6421 ggacgtcgtg gacggcatcg ggctcgaggc gctggtcgcc aagcgcacca ccggcctgat
     6481 gcagccgcac ctgaagagcc gcatcgaaaa gaccacgcgc aaggaatacc cgcagggcat
     6541 cgaggcctat ggcaccgatt cactgcgctt cacgctggcc agcctggcca ctcagagccg
     6601 ggatctgcgc tttgacctca atcgcgtcgc gggcaaccgc aacttctgca acaaactatg
     6661 gaatgccgct cgttttgtga tgctctcgtc ggagagctgc cccgctggcc caagctcggc
     6721 cgcggacttc agcgtcgctg atcgctggat tcgctccaga ctgggcgaga ccatccgtac
     6781 ggtcgagcgc gcgttcagcg agtatcgatt cgattttgcg gcatcggcgc tttacgaatt
     6841 cacctggcac gaattctgcg actggtatct cgaaatcgca aagccagtac tgcaaaactc
     6901 tgccgacgcg cgggcacagg cgtccgctcg tggcaatctg ctgaccgtac tggagtcgct
     6961 gctgcgcacg ctacacccgc tgatgccatt cattacggaa gaaatctgga tgcgagtcgc
     7021 cccgatggcc ggtgtcgggg gcccgagcat catgctctcg ccgtggccca aagcagatga
     7081 attcgcgccc gatcccgacg ccgacgagga aatgcactgg gtgatgcagg tcgtgctcgg
     7141 catccggcag attcgcggcg tactggacat tcccgcttcg cggaaattgc cattgctgct
     7201 gcacaacgcg agcgcgcgcg atctgcaatg gctggcgaaa caccgggtgc ttctcatgag
     7261 gctcgccggc ctcgagagca tgcaacccct ggcccacgca gaggcggcgc cccctgcagc
     7321 cgcggaactg gtgggcgagc tggtgctgct cgtgccaatg gcgggattga tcgacccggc
     7381 aagcgaattg gagcgcctcg agaagcgcct ggcaagagtg gaagcggaat tgaagagctt
     7441 ccgcggcaag ctggccaatg agaactttgt gcgcaatgcg ccgcccgaag tcgtagcgca
     7501 ggaacacgag cgcgtcagcg atcgggagcg tgcgcgcgcg ggactgatcc gccagatcga
     7561 gcaagttcgc aagctgttga actagacatg agccggatca gcgacacggt ttgcgcagtc
     7621 gagcgggtca tcctcggcaa gtcgctgcag gtgcggttgt gtctcacgtg cattctggcc
     7681 cgcggtcatc tgctcatcga ggacgtgccc ggcgtgggca agaccacgct cgcacacacg
     7741 ctggcgcatg tgctcggcct cgagtggacc cgagtgcagt tcaccagcga catgctgccg
     7801 gccgatattc tgggcgtgtc gatcttcgat cgcagcaccc acaagtttca gtttcgccgc
     7861 ggcccgattt tcactcagct gctgctggcc gatgagatca accgcgccag ccccaaggcg
     7921 cagagcgcgc tgctggaagc gatggaagaa cggcaggtga ccgtggatgg cgaaacctat
     7981 ccgctgccag agcctttctt cgtggtcgca acgcagaatc cgcatgagca gatcggcact
     8041 tacgggttgc ccgagtccca actggaccgc ttcctgatgc gagtgagctt gggatacccg
     8101 gatcgggcat ccgaacgtcg ggtcctgttg caaggtgagc gcaaggaaat cctcgagcgc
     8161 acgccttcgg tgctgaatgg cgaacaggtg ctggccttcc agcgcgcggc gcgcagcatg
     8221 cacgttgccg atgcgctgct cgactacgtg cagtcactga ttgcacgttc gcgtacgcag
     8281 atgtcgctcg gattgtcccc gcgcgctgcc cagggattgc tgcgcgcagc ccaggcctgg
     8341 gcattgctcg gcggggagaa agcggtgctc ccggaacacg tgcaggctgt gttcccggcg
     8401 atcgcggcac atcggttgga accgagggag agcgatggca gctcggacgg caatcagatc
     8461 gccgatggac tcatcaaagc cgtcccggtt ccggtttgac gcacgcggcc ggctcgcggc
     8521 ccgggcgcag cgctgggcgc aagcccggca gggtgatgac agcccgccgc tgacgctgct
     8581 gcagcggcgc atctacatcc taccgaccaa agcgggggtc agcacctccg ttctgctgtt
     8641 cgcgatgctg ctcgcgggaa tgaactacgg caacagcctt gcgctgtttc tgtgcttcac
     8701 cctgagcgga gtggccctgg tatccatgca cgagtgccat cgttcactcg ccggtctgaa
     8761 gctgctgcgg gcgcacgtcg aaaacagctt cgccgggcag gtcggcgagc tgcagctcta
     8821 cttcgagaat gtggacacgc ggctgcgcga ttcgctgagc gtgaagtgct caccgtgcga
     8881 tgaggtcgcc tttcgcgtcc aaccgggcga gatcaagccg gtgactgtga acttccttgc
     8941 caggacgcgc ggtcggcagc ggattgaccg gctgcgattg tcgaccacgg caccgatggg
     9001 actgtttcgc gcgtgggcat ggatccacct gccattggag gccatcattt acccggccgc
     9061 taccggcaac cgggcgttgc cgccgcggca gggagaaccg cgccttggcc ggcagctgac
     9121 gcacaacacc ggcgaggaag aatgggcctg gttgcgcaac taccagaaca gcgacccacc
     9181 gcgcagtgtt gcctggaagg cctatgcgcg cggcgcaccg ctgatggtgg cgcactacga
     9241 ctctccggcg ggcatgcagc gcacgctgcg cttctcggcg gttcctggca attcggtcga
     9301 gcaaaagctg tcgcagctga ccaggtgggt actggagtgc gagcgactgg gtgaggacta
     9361 cgcgctggag ttgcccgaag ccacgcaggc ctcgggccac ggcgtcgcac atcggcgcgc
     9421 gtgcctcgag agcctcgcgc agtacggtct atgacagaac ctgccgtgcc cgccagaacc
     9481 gtgtggttgc tgcttggcgg ctatctgggc gccgtgctgc tcaacgtcca tcacacggcg
     9541 ctctggtgtc tgccgctcgc gctgggcgtg gcaatctggc gggcgcgccg cttgcgcacg
     9601 tcggccggca agtcgcgccg tctggcacgt accgtggtca tcgtcatact cactgcaatc
     9661 gtgctgatcg gatttcgcac gctcaacggc gtgcaggccg gcgccagtct gctggtggcg
     9721 atggcggcac tgaagctgac cgagacctcc gctcggcgcg actggctgat catggtcggt
     9781 gcttctttgt tcctgctgct ggccgcttgc ctggatgcac agatgctatg gcgcctgccg
     9841 ctatacgtcg ccgagctatg tctgctgtgt atggggatgt acgccctggg cgcgggcgat
     9901 caagtacctt cgctcgcgcc actgacccat cgtactgcag gcactttgct ggccgcgatg
     9961 ccgctggcga tcctgctgtt cctgttcgta ccgcgcgtgg ccggctcgtt ctgggcgctg
    10021 ccgcggtcgc atgaggccac caccgggctg agcgatgaaa tgaaccccgg caacatttcc
    10081 tcgctgagtg tgtcgggcga tccggcggcg cgggtccggt tcgacggccc tccaccccca
    10141 cccgagcaac gctactggcg cggtttcgtg ctggataagt tcgatggcaa tacctggcga
    10201 cgcagtcggc gcgactatgg tttcgcaccg ccgctcacct acctgggcac ctcctatcag
    10261 tacgaagtct cgctcgaggc cagccagctc acggtcctgc tggcgctcga actgccgcag
    10321 ggcgcgcccg aatccttggg cgatgcccat ctgagcgatg actttcaggt gatcgactca
    10381 cagccggtca cgcaacgtct gacctatcga atgcagtcct atccgctgca ccgaagcctg
    10441 ccgcagcaac agctcacccc ggaggagcga cagcgctact taggctttcc gggcgcacgc
    10501 aacctgcgca gcatcgagct cgcccatacg ctgcgcgcta gcgccgacag cgacctcggc
    10561 tacatccgca gcgtgctcga ctacttcgcc cgcggcggct tcaagtacgc cctggatccg
    10621 gaactgtcca acggcaactc ggtggatgac ctgttgttca atacgcacga agggttctgc
    10681 gggcactacg cctcagcatt cgcgcagctt atgcgggcgg cgcgaatccc ggcgcgcgtg
    10741 gtgaccgggt atcagggagg gatctggaac cgctatggca actacctgtt gatccggcaa
    10801 tccgacgcgc acgcctgggc cgaagtctgg ctcgacgcta gcggatgggt tcgcatcgat
    10861 ccaacggccg tcgtcgctcc gaatcgtctc acccggggct cggacgacct cacatccaat
    10921 gccacgctgg ccgcgcggat gttccgctgg caatggctcg ccaacaccgt gcaggcctgg
    10981 caggcggtca acgcctggtg gcaggatgaa tttgtcggct tcaacttcag caagcagcag
    11041 gacctgctcg gacttctggg catccagcga cactacctgc gggccctggc cgtcatgctt
    11101 gcgatcggcg gcagtatttg gctcggcgtc atcgcctgga gtctgcgacc gcgtcattca
    11161 cggcgcgccg aggatgtgct cggccgaacc tggcgacttt tggaacgcaa acttcagcgc
    11221 gcggcctcgc cacgagcgtc gtatgaagga ccggtcagct acgcgcaacg tatcggcctg
    11281 gagcgacccg agctcgcgag ccgggtgagt gcactggcac gtcgctacgc ttggctgcga
    11341 tacggcccca aaccgagcaa cgaggaagtg gagcagtttc gccgcgccgt gcgtctgttg
    11401 aagacgatgc cggcgcggat cacgcgttaa gccatcgcgc agacagagcg ctctggcgcg
    11461 cgcacgcgcg ccagcagcag cccgccgcca atgaagaaga attccagcac caggatcgac
    11521 aggcgctgat tgcctgtcac catcgcgacc aatcccatcg cgagcggacc gagcactgcg
    11581 gcgaatttgc cgagcatgtt gtagaacccg aagtattcgc cggccctgtc cgcggggatc
    11641 aggcgcgcga aatacgaacg cgacagggcc tgcacgccgc cctgcaccga gccgaagatc
    11701 gcggccagaa tgtagaactg ctgcaccgtc tgcatgaagt aggcatagaa cgtgacgccg
    11761 acgaacactg ccagacccag atagatcgag cccttggtgc caaagcggtc tcccagccag
    11821 ccgaacagca gtgcaaacgg aaaggcgatg aactgcacga tcagcagcgc gacgatcagc
    11881 gaatcggaag ggaacccgag cttgctgccg aaatccaccg ccatcagctg caccgtattc
    11941 aaggcatcga tgtacagcca ataggcgagc agaaagttcc gcaccatgcg ctcgcgcatg
    12001 accttgcgaa tcgtggccca tagctcagcc actcccgatg gctcaccggt cggacggcct
    12061 tcgggaacat gtcggaacag cggcagcgtg aacagcagcc accagactgc gacgtcgagg
    12121 aatgcaatgc gtgtggcctg cgtcccgttg gcgagatgaa accagctcgg atgcagcgcc
    12181 atcagcacgt tgaccagaaa cagcagtcca ccgccgatgt agccgaacgc gtagccttgt
    12241 gccgaaacgc gattgatctc gctggcgtcg gccacctgca cgatgagcgc gtcctgaaag
    12301 gagctacccg cccagaacgc cacggtaccg accccgtaca ccaccagcgc ccagacccac
    12361 tcaccctgcc ccaccgtcgc cagcaccgcg ctgccgatcg atccgaggac ggtccagatg
    12421 ccgagccaac gcttcttgtg tccgcgccgg tcggccaggg ctcccagcca cggcgcgagc
    12481 agcatcacta tgaaagtagc cgttgagttc gccagtccca gatagaacgt ctgctgcgtg
    12541 cccgacagcg acgtcgcgac gtacttgtcg aagaagatgg gaaagaatcc gaccgcgatc
    12601 gtggtgatga aaccatgatt cgcgcacgcg gagagtgacc atgaccactg agccagatgt
    12661 ttgctagtca cggcgttctc ttgagcaatc cttggatcgc tattgcatca gatattcgcg
    12721 cgtctcgtga aactgaattt ccggccagcg ctcgcgggtc agctgcagat tcacccgtga
    12781 gggcgccaga tacaccagcg cgccgccatg atccagcgcc agatgttcgt aggcgcgggt
    12841 ccggaactcc tccagcttac cctcatcggc tgcagtaacc cagcgcgcgg tgtgcaccgt
    12901 cacactctca aacacacatt gcacgttgta ctcatcctgc aggcggaatg tgaccacttc
    12961 gaactgcagc tgtccgaccg cacccaggat cagctcgctg ttccggagcg gacgaaacag
    13021 ctgcgtcgcg ccctcctcgc acagctgatc caatcccttc gacagcgctt tcatgcgcag
    13081 tggatccttc aggaccgcgc gtctgaacat ttccggcgca aaattgggaa tgcccgtgaa
    13141 gtgcagcgat tccccttcac tgaagctatc gccgatgttg atcgtgccgt ggttgtgcag
    13201 gccgacgatg tccccggcat acgcctcctc ggcctgcgag cggtcagcag ccatgaacgt
    13261 cagcgcatcg gcaatgcgca tttccttgtc gaggcgcacg tgcttgaggc gcatgccgcg
    13321 ttgatagcgc ccggaacaga gccgaagaaa tgcgatccgg tcgcgatgac caggatccat
    13381 atttgcctga atcttgaaga caaagccgga cagcttcggc tcctgcgccg agacgctgcg
    13441 ctcacggctg gggcgcggca gcggtgccgg tgcgtgcgtc acgaacgagc gcagcagttc
    13501 ctcgaccccg aagttgctga tcgccgagcc gaagaaaacc ggcgtctggc gagcagctcg
    13561 atacgcctgc gcatcaaacg ccgcggtggc gccgcgcacc agttcgatct catccgcaaa
    13621 ggcctgcgcc gagtcgccaa acagttccgc cgccgccggc gaatgcagtc cgtcgatcac
    13681 ccgattgccg ccgacgcgcc cgcgctcgcc ggcctcgtag acgtagatgc ggtcctccgg
    13741 cagatggtag atgcccttga gttcgcgtcc catgccgacc ggccaggtaa ccggcgcaca
    13801 ctgaattttc agcacgcgtt cgatctcatc cagcagttcg atcggcgccc gtccgtcgcg
    13861 atccagcttg ttgatgaagg tcatgatcgg tgtgtcgcgc agccggcaca catccatcag
    13921 cttgatggtg cgttcctcca cgcccttggc gcagtcgatc accatcagtg ctgagtcgac
    13981 cgcggtgagg gtgcggtagg tatcctcgga gaagtcctcg tggccgggag tgtcgagcag
    14041 attgatgaca tgatcccgat agggaaactg catcacggaa ctggtgaccg agattccgcg
    14101 ctgctgttcc agtcgcatcc agtcagaggt ggcgtggcgt gcggctttgc gtcctttcac
    14161 agttccagcc atcgcgattg cgccgccaaa cagcagcagc ttttcggtca gagtggtctt
    14221 gccggcatcg ggatgcgaaa tgatggcaaa cgtgcgccgg cgcgcgattt cctgctcgag
    14281 cgtagtcatg agaaggcgcg cgattctaat cgatgtggcg cttcagcacg atggcatctt
    14341 cgcgtccgtc cacggcctgg tagtagccac ggcgcacgcc gatctgcaca aaacccagcg
    14401 actgatacag ccggatggcg gaagtgttcg aaggtcgagc ttccagaaag gcctcacgcg
    14461 cgccgaccgt ggcggccacc tgcagcaggt gcgccagcag gcgccttccg ataccacggc
    14521 aacgatagtc ctcgccgacg cacagattca gcagatgggc ctcgccggcc gccacgctca
    14581 tgatgccgta gcccgcgaca gcgacaccga tctcggccac gcggcaggtg taattcaccc
    14641 gcaggcagtc gcggaaaatg ccctcggtcc aggggaacgc ataggacaga cgctcgatgc
    14701 gcagaatctc cagcacgtcc gattcacggg cgctgcgaaa actcagctca ggcatcgcca
    14761 ccgcgtctag tcgaaccacc gccataagag gtgctaattc cctcgcgccc gcgccagttc
    14821 ggtgcgcgcg aacttcagat ccgtccaaac cttacgcttc tctccgggac tgcgcagcag
    14881 gtaagcagga tgataggtga cgatcagtgg cgtggcacgt tcgcccaagc gatgcacctg
    14941 cccgcgcagc cggccgaccg aaacgtccgt agccagcagg ttctgcgctg caataccccc
    15001 caccgccaaa atcatgcgcg gctgaatcag atcaatctgc tgctgcagat agggcagaca
    15061 ggaggcgact tcagtcgggt tcggattgcg gttgttgggc ggccggcact tgagaatatt
    15121 ggcaatgtag acccgctcgc gaggttgccc catcgcctgc agcatcgaat tgagcaactg
    15181 cccggcgcgg cccacgaacg gctcgccctg ttcgtcctca tcctggcccg gcgcctcacc
    15241 aatcaccatc agctccgccc ggcgctgacc gactccgaac accgtttgcg tgcgcgtctg
    15301 gcacaagcca caacgtgtgc actcacgcac tcttgcctgc agctcatccc agacagcgcc
    15361 ggcctgcggc ggcgctgggg ccgtggcgaa acgcggcacc cagacatcca gccccagggc
    15421 ctgcagatac tgttcgcggg ctctcacgtg agctcaggct tccggcgtga cagcgcgcac
    15481 cgggcgtttg ccacggcgca gccggcgcca tacccacatc aacggcgaat acgccgcgta
    15541 gctgccgaaa aacaccagca gtacgatttc gcgcgcgcca ccgatcacga caaagatcgc
    15601 agcgatgacc accagccaca cgaagctcac cggtcgcgtc cagtccaatc ccttgccgct
    15661 gctgtaagga aagcggctga ccatcaaggc ccctgccgtc gctgtaatgc caaacgcaag
    15721 aatcagaccc ggcagaccgg gttcgcgcca ccattcactc gaggtccata gaaacgctgc
    15781 aaccactgcg gccgccgacg ggctgggcag cccctcgaaa taacgcttgt cctgaacccg
    15841 gctgttgaac tttgccagcc gcatggccgc cgcggcggcg aagaagaacg cggccagcca
    15901 gccaaagcga aaccactcct tgccgtattc ggcaatgcga gcgacgcccc actgatagga
    15961 cacgatcgcc ggcgccatac cgaaggccac catgtcagca agactgtcgt attccttacc
    16021 gaacgccgac tcggtgcggg tgaggcgcgc gacgcgtcca tccagaccat cgaaaatcat
    16081 cgcaatgaag accgcgacac cggcgcgttc gaaatttcca tcgatggcgg cgacgatggc
    16141 gtagaagccg gagaacaggc aaccggtcgt cagcaggttt ggcagcaggt agatgccgcg
    16201 ggccggttgg cgtgcgggcg gactctctgg atccatggct caggcatcat agcctatctg
    16261 ttagcattac tgtttttacc acgcttaggc ttctcatcac gcatgcgtct gtcgcaatct
    16321 ccaatcgcca ccgtcaagga aaccccatct gaggccgaga tcgtcagcca tcagctgatg
    16381 ctgcgcgcgg gaatcattcg ccgcctggct gccggtatct atacctggct gccgctgggg
    16441 ctgcgcacgc tgcgcaaggt tgaaaacatc attcgccagg agatggaccg ggcaggcgcg
    16501 ctggagatgc tgatgccagc ggttcagcct gcagagctat ggcaggagtc gggtcgctgg
    16561 atcaagtacg gtccggagct gctgcgtctc aaggaccgtc accaacgcga cttcgtcgtc
    16621 ggtcccacgc acgaggaagt gatcacggac atcgcacgcc gcgaactgaa gagctaccgt
    16681 cagttgcccg tgaatttcta tcagatccag accaaattcc gcgacgaggt gcgtccgcgg
    16741 ttcggcgtca tgcgcggtcg cgagttcatc atgaaggatg cgtattcctt ccacgtcaac
    16801 caggcatcgc tgaacgaagg atacgcggcg atgagtgctg cctattcgcg catgttcaag
    16861 cgaatgggcc tgaactttcg ggcggtcaga gccgactcgg gtgccatcgg cggcaacgcc
    16921 tcggaggaat ttcaggtgct cgccgagtcc ggtgaggatg cgatcgcagt ctctgacggc
    16981 gatgacttcg ccgccaatat cgagctagcg aaggcactgc cgcccgccgg gcctcgcccc
    17041 gcgcccggca aaccgatgac tcgcgttgcc acgccgggag cgaagacgat cgaacaggtc
    17101 agcgaattgc tgcaggtgga acctgcgcaa tgcctcaaga ccctgatcgt cgccggcagt
    17161 gaaggcactg cggttgtgct gctgat
//
LOCUS       DATMRM010000346         3819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2675909, whole genome shotgun sequence.
ACCESSION   DATMRM010000346 DATMRM010000000
VERSION     DATMRM010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2675909"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VNZ06_10285"
     CDS             <1..311
                     /locus_tag="VNZ06_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWW21182.1"
                     /translation="AHVGVVTPLRDGMNLVAKEFVAAQDPEDPGALVLSKMAGAANEL
                     GGALLINPYDKNAVARALRQALSMPLAERRERHETNLKAVRANSISRWHQSFVSRLAA
                     "
     gene            311..2326
                     /locus_tag="VNZ06_10290"
     CDS             311..2326
                     /locus_tag="VNZ06_10290"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HWW21183.1"
                     /translation="MSQPQSQSRLDPRPPVAAGSLAAIIAPRVVIERLTPFVDGGRFP
                     AKAVVGQTVVVEADIFIDGHEKPGAQLLWRSAHEPTTQVVPMRPLGNDRWRAEFTPSV
                     PGAHSFNIEAWLDVWASYRSDLLKNAQAGAVNPLDIQEGREQVLAALQRTPERVSSAA
                     ADWEHRLADADIVQGRELLSSEELDAMMRAYGERRFITRLRSELLVDVERAAAGFASW
                     YELFPRSQTRSAAQHGTFDDVISRLPAIRSMGFNVVYFPPIHPIGVSHRKGRNNALTA
                     APSDPGSPYAIGAPAGGHDSVHPLLGGITAFRRLVAAAHEYDMEIALDFAVHCSRDHP
                     WLTEHPGWFSWRSDGSIRYAENPPKKYQDIVNFDFYAKDAKPSLWLALRDVVQGWVDE
                     GVRMFRVDNPHTKPVPFWQWLIADIRARTPEVIFLAEAFTRPAMMYRLGKIGFSQSYT
                     YFTWRNSKHELTEYFTELTTQPTKDFYRPHLFVNTPDINPYFLQTSGRPGFLIRAALA
                     ATLSGLWGLYSGYELCECAPLPGREEYRDSEKYEIRVRDWNAPGNIISEISRLNYIRK
                     LNPSLHSYLNLKFYNAWNDQILYYGKSTTDLSNFVLVLVSLDPHQVQETHFEAPLWEF
                     GLPDSAALEVEELMRGQRFIWRGKLQHWRFVPQEMPFAIFRIRPIKG"
     gene            2332..>3819
                     /gene="treS"
                     /locus_tag="VNZ06_10295"
     CDS             2332..>3819
                     /gene="treS"
                     /locus_tag="VNZ06_10295"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HWW21184.1"
                     /translation="MDVLASKDKVRRRRIPRHEFSADPLWYKDAVIYQLHVKSFYDAN
                     GDGIGDFEGLIQKLDYIAELGVNTIWLLPFYPSPRRDDGYDISDYTNVHPDYGLRADA
                     KRCISEAHARGLRVITELVLNHTSDQHPWFQRARNAPKGSWARDFYVWSDTDTRYAGT
                     RIIFLDTEKSNWTWDPVANAYYWHRFYSHQPDLNFDSPRVVNAVLAVLRFWLGVGVDG
                     FRLDAVPYLVEREGTNNENLPETHSMLKRIRAELDRTNPGKMLLAEANQWPEDVQQYF
                     GNGDECHMAFHFPLMPRMYMALAQEDRFPITDILRQTPDIPASCEWAIFLRNHDELTL
                     EMVTDRERDYLWRQYAADARARLNLGIRRRLAPLMQRDRRRIELLNSLLLSMPGAPVI
                     YYGDEIGMGDNIHLGDRDGVRTPMQWSLDRNGGFSRCDPAAAPAPPIMDSLYGFQALN
                     VEAQARDSHSLLNWMRRMLTIRKQQQAFGRGTLRLLYPTNRRVLAY"
BASE COUNT          712 a         1191 c         1199 g          717 t
ORIGIN      
        1 tcgcccacgt gggcgtggtg acacctttgc gtgacggcat gaatctggtg gccaaggagt
       61 tcgtcgcggc acaggatccc gaagatccgg gggcgctggt gctgtccaag atggctggcg
      121 cggccaacga gctcggcggc gcactgctca tcaatccgta tgacaagaat gcggtggcgc
      181 gagcgctgcg acaggcactg tcgatgccgc tggcagagcg tcgtgagcgg cacgagacca
      241 atctgaaagc ggtgcgtgcc aacagcatca gccgctggca tcagtctttc gtttcgaggc
      301 tggccgcctg atgtcccagc cgcagtcgca atcgcggctc gatccgcggc cgccagtcgc
      361 ggccgggagc ctcgcggcca tcatcgcacc gcgcgtcgtg atcgagcgtc tcacgccgtt
      421 cgtggacggt gggcgcttcc ccgccaaggc ggtggtcggt cagacagtgg tcgtcgaggc
      481 cgacatcttc atcgatgggc atgagaagcc cggcgcacag ctgctgtggc gttcggcgca
      541 tgaaccgacg acgcaggtcg tgccgatgcg gccgctcggc aacgatcgct ggcgcgctga
      601 gttcacaccg agtgtacccg gagcgcacag tttcaacatc gaggcctggc tggatgtatg
      661 ggcgagctat cgcagcgacc tgctgaagaa tgctcaggcg ggagccgtca atccgctcga
      721 tatccaggag gggcgcgagc aggtgctggc agccctgcaa cgcactcccg agcgcgtctc
      781 atccgccgcc gctgactggg aacaccgact ggccgatgcg gacatcgtgc agggccgcga
      841 gctgctgagt tccgaggagc tggatgcgat gatgcgcgct tacggcgagc gtcgcttcat
      901 cacgcgtctg cgctcggagc tcctggtcga tgtcgagcgc gcggccgccg gctttgccag
      961 ctggtacgaa ctgtttccac gttcgcaaac gcgctcggcc gcgcagcatg gcacgttcga
     1021 tgacgtgatc tcgcgcctgc cggcaatccg ctcgatggga ttcaatgtgg tgtacttccc
     1081 gccgatccat ccgatcggcg tgtctcatcg caaggggcgt aacaatgcgc tgaccgcggc
     1141 gccttcggat ccgggcagcc cttatgccat cggcgcgccg gccggtggac atgattccgt
     1201 gcatccgctg ctgggcggca tcacggcgtt tcgccgcctg gtggcggcag cgcatgaata
     1261 cgacatggag attgcgctgg atttcgcggt gcactgctcg cgtgatcatc cctggctcac
     1321 cgagcatccg ggctggttca gctggcgcag tgacggctcg attcgctatg cggagaatcc
     1381 gccgaagaag taccaggaca tcgtcaactt cgacttctac gccaaggatg ccaaaccctc
     1441 gctgtggctg gcgctgcgcg atgtggtgca gggctgggtc gatgagggcg tgcgcatgtt
     1501 ccgcgtcgac aacccgcaca ccaaaccggt gccgttctgg cagtggctga tcgccgatat
     1561 ccgggcgcgc acgccggaag tgatctttct ggccgaagca ttcacgcgcc cggcgatgat
     1621 gtatcggctc ggcaagatag gattttcgca aagctacacc tacttcacat ggcgcaatag
     1681 caaacacgag ctcaccgagt acttcaccga gctcacgacg cagccgacga aggatttcta
     1741 ccggccgcac ctgttcgtca atacacccga tatcaatccg tacttcctgc agacctccgg
     1801 ccgaccgggc tttctgatcc gcgcggcgct ggccgcgaca ctgtcgggcc tgtggggact
     1861 ctatagcggc tacgaactgt gcgagtgcgc gccactgcca gggcgtgagg aataccgcga
     1921 ctccgagaag tatgagatcc gggtacggga ctggaacgcg ccgggcaaca tcatcagcga
     1981 gatctcgcgt ctcaactaca tccgcaagct caatccgtca ctgcatagct acctgaatct
     2041 gaagttctac aacgcctgga acgatcagat tctctactac ggcaagtcca cgacggatct
     2101 gtcgaatttc gtgctggtgc tggtgagtct ggatcctcac caggtgcagg agactcactt
     2161 cgaggcgccg ctgtgggaat tcggtttgcc cgacagcgca gcccttgagg tcgaggagct
     2221 gatgcgcggc caacgcttca tctggcgcgg caagctacag cactggcgtt tcgtcccgca
     2281 ggagatgccg ttcgcgatct tccggattcg tccaatcaaa gggtaggcgc gatggatgtt
     2341 ctggccagca aagacaaagt caggcggcgc cgcataccgc gacatgaatt cagcgccgat
     2401 ccgctctggt acaaggacgc cgtcatctat cagctgcacg tcaagtcctt ctacgacgcc
     2461 aacggcgacg gcatcgggga tttcgaaggg ctgatccaga agctggacta catcgccgag
     2521 ctgggcgtca acacgatctg gctgttgccg ttctatccca gtccgcggcg cgacgatggc
     2581 tacgacatct ccgactacac caacgtgcat ccggactatg gattgcgtgc cgatgccaag
     2641 cgctgcatct ccgaggcgca cgcacgcgga ctgcgcgtca tcaccgaact ggtgctcaat
     2701 cacacctcgg accagcatcc ctggtttcag cgtgcgcgca acgcgcccaa gggctcctgg
     2761 gcgcgtgatt tctatgtctg gtcggatacc gacaccaggt atgccggtac gcgcatcatt
     2821 ttcctggaca ctgagaagtc caactggacc tgggatccgg tcgccaacgc atactactgg
     2881 catcgattct attcgcatca gccggatctg aatttcgaca gcccgcgtgt ggtcaacgcg
     2941 gtgctggcgg tgctgcgatt ctggctcggg gttggggtcg atggattcag gctcgacgcg
     3001 gtcccctacc tggtggaacg tgaaggcacc aacaacgaga acctgccgga aacgcattcg
     3061 atgctgaagc gcatccgggc ggagctggat cgcaccaacc cgggcaagat gctgctggcc
     3121 gaggccaatc agtggccgga ggacgtgcag caatactttg gcaacggtga tgagtgccac
     3181 atggcatttc actttccgct gatgccgcgc atgtacatgg cgctggcaca ggaggaccgc
     3241 tttccgatca ccgacatcct gcggcagacg cccgacattc cggccagttg cgaatgggcc
     3301 atctttctgc gcaaccacga tgaactgacg ctggaaatgg tgaccgatcg agagcgcgac
     3361 tacctctgga ggcagtacgc cgcggacgcc cgcgcccggc tcaacctcgg gatccggcgc
     3421 cgtctcgccc cactgatgca gcgtgaccgg cgccggatcg aactgttgaa ttcgctgctg
     3481 ctgtcgatgc cgggtgcgcc ggtgatctat tacggcgatg aaatcggcat gggagacaac
     3541 attcatctgg gcgatcgcga tggcgtgcgc acgccgatgc agtggtcgct cgaccgcaac
     3601 ggcggcttct cgcgttgcga cccggcggca gcaccagcgc cgccgatcat ggattcgctc
     3661 tacggctttc aggccctgaa cgtcgaagcg caggcgcgcg attcgcattc gctgctgaac
     3721 tggatgcgac gcatgctgac gatccgcaag cagcaacagg cattcggtcg cggtacgctg
     3781 cgcctgctgt atcccactaa tcgacgggtg ctcgcctac
//
LOCUS       DATMRM010000347         9451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2980053, whole genome shotgun sequence.
ACCESSION   DATMRM010000347 DATMRM010000000
VERSION     DATMRM010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9451
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2980053"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2084
                     /locus_tag="VNZ06_10300"
     CDS             <1..2084
                     /locus_tag="VNZ06_10300"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW21185.1"
                     /translation="EAPNTQPRVGLASWLGGGSVFVVLLAVAAIAIACGILLNRLVQQ
                     QALVRAQLAVATARELLRRGGEDALSDARVLAERPTLQRLLETEPNSGLEPFLRRYAE
                     STDTDIAAVLRNDTPVGQSDSALDWPQIATALQEQGERFLIAPKNGGPVIWGAAAAIP
                     GTSLRAVTLKFTSNALLANLSRQVGAQLSVTNFAAYHGPPDDEMTPLHTQALTGPGGA
                     AAGKLRHGDRYAATIVLAAQTGEVVGLLDARLGSGEFATATRNFEWILAVVAIVVATI
                     AGFLGVLYGRWLAQPVVALRALADRMGRGDFSVAIPAVAPLEVDALARSMDEMRRNLV
                     NLTDTLRRREAEAQTVLSGVVEGVFAVDLERRIRYANPQVARLLQRPESEIVGQFCGD
                     VLQPQPHNGERPCAHNCPILAARDSPHSSSREMLCLRDGTTRSTIVLSSPPVQGVQVQ
                     ILRDETDLEAARRARDSVLGNISHEFRTPLAAQLASIELLRDGMKDMPREAQMELLSN
                     VERGVLRLMRLIDNLLESVRIEAGQDTIRLQPVDLLDVVIDARDLIAPLLRQRQLSVS
                     IDDRGIGAIVRGDAQRLGQVFVNLLSNAAKFAPEGSTLRLGGQRSGAQVEVWIEDEGA
                     GVPEGSSSAIFERFQRGLGTEPDAPGLGLGLWIVKSIVERHGGTVRMERTPERRTRFT
                     LSLPQDLEDDA"
     gene            2081..2752
                     /locus_tag="VNZ06_10305"
     CDS             2081..2752
                     /locus_tag="VNZ06_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWW21186.1"
                     /translation="MKLLVVDDDADMLAVVGFALRQAGFPVVSANSYGTALNVFRTER
                     PDLAILDINLPGGSGFDLCDVLRKESTSPIMMLTARGEEADLVRALELGADDYLTKPF
                     SPRTLVARVKALLRRAGHEGSSQTQVGDIALDQDLLLLRIGGREIKVTPLESRLLQLL
                     LANAGQVVHTDRMLVHVWGHRGGGDRQLLKQLVHRLRHKIGDDPDQPKWLSTVPSVGY
                     MLRVK"
     gene            complement(2783..3832)
                     /locus_tag="VNZ06_10310"
     CDS             complement(2783..3832)
                     /locus_tag="VNZ06_10310"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21187.1"
                     /translation="MNRKTLSATVAFLSGILLTLVFLELVLRMLPVGRGAYAAEPDPA
                     WPAHHLVPNSSYTFSSGWDLEDVQHGHINNMGYVSPFDYSRGTGGVFVVGDSFIEDQM
                     TRYDESLQGALPSLLKRPMPVLGFGTSGGNLAHDLGVATLIGQQFKPTWAVVVVTRRN
                     FVNGFVTQGGYYQWTSGDEPGIGRAPDARHGLLVDSMRHLALLAYMRGNLEADRSTLF
                     AAKKWQQANVCHGGVLSRSDEALVRFSAQQLPIRFKLDPSHVVLVLDTDRAAIYNPPS
                     VPCPSRDDLALALLGREAAAAGIQVVDMDPVFREAYRLTGRRFDYLPVDGHWNPSAHA
                     LAAQQVARIINGATP"
     gene            complement(3838..5322)
                     /locus_tag="VNZ06_10315"
     CDS             complement(3838..5322)
                     /locus_tag="VNZ06_10315"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HWW21188.1"
                     /translation="MLFTTGVFLFLYLPITLAVFFICARLFGRSTAAFWLALASLFFY
                     GYWMPAYTLLLLASVIVNYAFGTLIGRSSGGTRRLGLIVAVAANLCTLAYFKYANFLL
                     QSVNYVASTHIPILDVILPLGISFFTFTQIAYLADVYAGKVQERNPIHYLLFVTYFPH
                     LIAGPVLHHAEMMPQFRLPENYRPRLEPFAIGLTFLTIGLAKKILVADSIAPIANDAF
                     DNRISQGIGVAHAWLGVSAYAFQLYFDFSGYSDMAVGLSLLVGVRLPYNFDSPYKARS
                     IIDFWRRWHMTLSRFLRDYLYFALGGNRLGKLRRHLNLMVTMLLGGLWHGASWTFVVW
                     GGLHGIYLVINHGWRYLLERLSAVRSASRPSWQSFLGQASSTLLTLVAVLIAWVFFRA
                     TSIDAALRLVSSMFVQPPHSSMSAADWHLLPWIVALSVVVLVAPNSQELIDGRVGTRL
                     LAWRSMRRFHAMLAFVVGVELVAIVLLAMIAARRSTAEFIYFNF"
     gene            5700..5912
                     /locus_tag="VNZ06_10320"
     CDS             5700..5912
                     /locus_tag="VNZ06_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21189.1"
                     /translation="MKIFDADDREMMRITSVSREGSQLVIQGMIFGSMPLTAKLRPEE
                     ARAAFRLMDLRTTWFLLTLLLRRRRD"
     gene            complement(5920..6387)
                     /locus_tag="VNZ06_10325"
     CDS             complement(5920..6387)
                     /locus_tag="VNZ06_10325"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HWW21190.1"
                     /translation="MTRARVLWLILTAAAVSGCSTPRFVIEAENARTNTHIVLAFEET
                     VFSKHHVREGFSRYVAPEYKEHDPAFPGGQDEAARGLVDQLTNEYPDSRVVVKRTVAQ
                     RDLVSVHAFWDQKPGATRGLVRVDIYRLVNARIVEHWVVEQAVPSGSDAEAMF"
     gene            complement(6387..7361)
                     /locus_tag="VNZ06_10330"
     CDS             complement(6387..7361)
                     /locus_tag="VNZ06_10330"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HWW21191.1"
                     /translation="MMARAIRIYQHGGPEVLRCEQVEVGTPGEQEVLVRHTAIGINFA
                     DVYLRIGLYPQPLPSGMGSEAAGVIEAIGRKVRGFKPGDRVAYSYPVAGAYSEHRVLP
                     AGALIRIPAGVDAEQVAAVLLKGMTCWFLLRQTYKVGRGDVVLVPAAAGGVGLILCQW
                     ARSLGAKVIGVVGSPDKATLAKRNGCHQVLVGYEDMAARVRKLNRGAGVAVVYDGVGR
                     DTQQASLDSLKPRGMLVSFGNASGPAAPLAPSELAKRGSLFFTRSRMADYVATAETRQ
                     TASRELFALMRRKSLRVHIGQRYALAEAAQAQRDLESRRTFGSSVLVP"
     gene            7496..8209
                     /locus_tag="VNZ06_10335"
     CDS             7496..8209
                     /locus_tag="VNZ06_10335"
                     /inference="COORDINATES: protein motif:HMM:NF025867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21192.1"
                     /translation="MPRPQLRKRPRAVGVSGRTPRIATRKRRGIGRPAAGSQTVGRDA
                     LIARTCEMLTELPPNRITRAEVARHMNVDPSLIRYYFRDRSTLLVAAAERLIETFQRL
                     LDEELAQSDMSAAGRLRARISALLKLNINYPFFHRLMIDELVKIESRPAQRFLEELTH
                     KGVVGYGAIVDAGVKEGSFRRVNSAFLFLAVIGVCEFFVNGMPILRIAMGKEFDERAI
                     AARYRDFICELLTTGLKSK"
     gene            8280..8867
                     /locus_tag="VNZ06_10340"
     CDS             8280..8867
                     /locus_tag="VNZ06_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWW21193.1"
                     /translation="MSKTTGDRLPPIPDDQQTPAQRKVVKALLAGSRKAVRGAFIALL
                     RSPDLCDRTQKLGEYLRYRSVVPIKLRELAILATARHWQQTYEWQAHATSAVEAGLPE
                     QVIDTLALEWDGAGLAPDEHTVLRFCRELHRDRVVSDRTYDAAKTLLGEKGVVELCGI
                     CGYYAMLAMVMNVAHTPPVSEGPPVPFSPPEAKTA"
BASE COUNT         1855 a         2992 c         2909 g         1695 t
ORIGIN      
        1 ctgaagcccc aaacactcag ccgcgtgtcg gcctcgcctc atggcttggc ggtggcagcg
       61 tgttcgtggt gctgctggcc gttgccgcca tcgcgattgc ctgcgggatt ctgctgaatc
      121 gtctggtaca gcagcaggcg ctggtgcggg ctcagctcgc ggtcgcgacc gcgcgcgagc
      181 tgctgcgacg gggaggcgaa gatgccctga gtgatgcgcg tgtgctggcc gagcgtccaa
      241 ccctgcagcg cttactggaa accgaaccaa acagcggcct ggagcctttc ctgcgtcgct
      301 acgccgaaag taccgatacc gacatcgccg ccgtgcttcg caacgacacg ccggtggggc
      361 agtcggattc ggccctcgac tggccacaga tcgccaccgc gctacaggaa cagggagagc
      421 gcttcctgat cgccccgaaa aatggtggac ccgtcatctg gggcgccgcc gccgcaattc
      481 cgggcacctc gctgcgggcg gtcacactga agttcacatc caatgcgctg ctggccaacc
      541 tcagccgtca ggtcggtgca caactcagcg tcacgaattt tgccgcctat cacggtccgc
      601 ccgacgatga gatgacgccg ctgcatacgc aggcactcac cggcccaggc ggtgcggccg
      661 cgggcaagct gcggcacggc gatcggtacg ctgcgaccat cgtgctcgca gcccagaccg
      721 gtgaagtcgt gggactgctc gatgcgcggc tcggcagcgg cgagttcgct accgcgaccc
      781 gcaacttcga atggatactg gccgtcgtgg cgatcgtcgt cgctacgatc gccggcttcc
      841 tcggggtgct ctatggacgc tggctggccc agccggtcgt ggcactgcgt gcgctggctg
      901 atcgcatggg tcggggggat ttttcggttg ccatcccggc ggtggcgccg ctcgaggtcg
      961 atgcgctggc gcgttccatg gacgagatgc ggcgcaacct ggtgaacctg accgacacgc
     1021 ttcgtcggcg ggaggccgaa gcccaaaccg tgctcagcgg tgtcgtggaa ggtgtgttcg
     1081 cagtcgatct cgaacgtcgc atccgttacg ccaatccgca agtcgcaaga ctgctgcagc
     1141 gacccgagag cgagatcgta ggtcagttct gcggcgatgt actgcagcct cagccacaca
     1201 acggcgagcg cccttgcgca cacaactgtc cgatactggc ggctcgagac tcaccgcata
     1261 gcagctcgcg cgaaatgctg tgcttgcggg acggcacgac gcgctcgacc atcgtgctga
     1321 gctccccgcc agtccagggc gtgcaggtgc agatcctgcg cgatgagacc gatctggagg
     1381 ccgcacgtcg cgcccgcgac agcgtgctcg ggaacatttc gcacgagttt cgaacaccac
     1441 tggcggcgca gctcgcctcg atcgagctgt tgcgtgacgg catgaaagac atgcctcgcg
     1501 aagcgcagat ggagcttctg tccaatgtcg aacgcggggt gctgcgcctg atgcgcctga
     1561 tcgacaacct gctggagagc gtgcgcatcg aggccggaca ggacacgatc cgactgcagc
     1621 cggtagacct gctggacgtc gtgatcgacg cgcgcgacct gatcgcgccg ctgttgcgcc
     1681 agcgtcagct gagcgtgtcg atcgacgacc gcggaatcgg cgcgatcgtg cgcggcgacg
     1741 cgcagcgcct gggccaggta ttcgtcaatc tgctgtccaa tgccgccaaa tttgctcccg
     1801 agggcagcac cctgcgcttg ggcggacagc gcagcggcgc tcaggttgag gtgtggatcg
     1861 aggacgaggg agcgggcgtg cctgagggaa gttcgagtgc catcttcgag cgctttcagc
     1921 gcggcctggg cactgaaccg gatgcgccgg gcctcgggct ggggctgtgg atcgtgaagt
     1981 cgatcgtcga acgccacggg ggtacggtgc gcatggagcg cacgcccgag cgtcgcaccc
     2041 gctttacgct gtcgttgccg caggatctgg aggatgacgc ttgaaactgc tggtagttga
     2101 tgatgatgcc gacatgctgg cggtggtcgg ctttgctctg cgccaggccg gatttcccgt
     2161 ggtatcagcc aacagctatg gcacggcact caatgtcttc agaaccgagc ggccggatct
     2221 ggcgatcctc gacatcaatc ttccgggagg atccggtttc gatctgtgcg atgtgctgcg
     2281 caaggaaagc acctcaccga tcatgatgct gaccgctcgc ggcgaggagg cggacctggt
     2341 gcgagcgctg gaactcggcg ccgacgacta tctgaccaaa ccgttcagcc cgcgcacgct
     2401 ggtggctcgg gtcaaggcgc tgctgcgccg ggccggccac gaaggcagca gccagacgca
     2461 ggtgggcgac atcgcactcg accaggacct gctgctgctg cgcatcggcg gccgcgagat
     2521 caaagtcacg ccgctcgagt ccaggttact gcagctgctg ttggcgaatg ccggacaggt
     2581 ggtgcacacc gatcgcatgc tggtgcatgt ctggggacat cgtggcggcg gggatcgcca
     2641 gctgctcaag cagctcgtac atcgcctgcg ccacaaaatt ggcgatgacc ccgatcagcc
     2701 aaaatggctg agcaccgtgc cgagtgtcgg ctacatgcta cgggtgaaat gacaggcgtt
     2761 gcgacacaaa agctcacaag gtctaaggcg tagcgccgtt gataatgcgt gccacctgct
     2821 gcgcggcaag cgcgtgagca ctcggattcc agtgtccgtc caccggcagg tagtcgaagc
     2881 gccgacctgt cagacgatag gcttcgcgaa acaccggatc catatcgacg acttggatgc
     2941 cggccgcggc cgcttcccgg cccaggagtg ccaaggcgag atcatcccgt gacggacaag
     3001 gaacagatgg cgggttgtaa atcgcagcgc ggtcggtatc caggaccagc acgacgtgcg
     3061 agggatccag cttgaaccgg attggtagct gctgcgcgct gaatcgcacc aacgcctcat
     3121 cggaccgcga gagcacgccg ccgtggcaga cgttcgcctg ttgccatttc ttggccgcaa
     3181 acagtgtcga gcgatccgcc tccagattgc ctcgcatata ggccaggagc gccagatgcc
     3241 gcatggaatc gaccagtagg ccgtgccgcg cgtccggcgc ccttccgatg cccggctcat
     3301 cgcccgaagt ccattggtag tatcccccct gcgtgacgaa gccgttcacg aagttcctgc
     3361 gagtgaccac cacgaccgcc caggtgggct tgaactgctg cccgatcaat gtcgccacgc
     3421 ccagatcatg ggccaggttg cccccggagg tcccaaatcc gagcaccggc atgggtctct
     3481 tgagcaggct gggaagcgcg ccctgtagtg actcgtcgta acgcgtcatc tggtcttcaa
     3541 tgaaactgtc gccgacgacg aacacccctc cggttccccg ggaataatcg aacggtgaca
     3601 cgtagcccat attgttgatg tggccatgct gtacgtcctc gaggtcccag ccgctcgaaa
     3661 atgtatagct ggagttgggt accagatgat gggcgggcca cgcaggatcg ggctccgccg
     3721 cataggcgcc ccggcccacc ggcaacatcc gcagcaccag ctccagaaaa accaacgtca
     3781 gcaggatgcc agagagaaac gcgacggttg cactgagagt cttgcgattc atggcgacta
     3841 gaagttgaaa tagatgaact cggcggtact tcgccgggcc gcaatcatcg ccagcagcac
     3901 gatcgcgacg agctcaacgc cgacaacgaa ggcgagcatc gcgtgaaacc ggcgcatcga
     3961 gcgccaggcc agcaggcgcg tgcccacccg accatcgatc agctcctgtg aattcggcgc
     4021 caccaggacc accactgaga gcgccacaat ccacggcaac agatgccaat cggcggccga
     4081 catgctcgaa tgtggcggct gtacaaacat ggagctgacc aggcgcaggg ccgcatcgat
     4141 gctggtcgca cggaagaaca cccaggcaat caggaccgcc accaaggtca gcagtgttga
     4201 agatgcctgt ccgaggaatg actgccatga gggcctggat gccgagcgca ccgcactcag
     4261 gcgctcgagc agatagcgcc aaccgtggtt gatcaccagg tagatcccgt gcagtccacc
     4321 ccatacgaca aaggtccacg aggcgccgtg ccacagtccg ccaagcagca tggtaaccat
     4381 cagattcaga tgccgccgca gcttacccaa ccgattcccg cccagcgcga aatagaggta
     4441 atcccgcagg aagcgcgaca gggtcatgtg ccaacggcgc cagaagtcga tgatgctgcg
     4501 cgccttgtaa ggtgagtcaa agttatatgg aaggcgcaca ccgaccagca gcgagagtcc
     4561 aaccgccata tccgagtagc ccgagaagtc gaaatacaac tgaaacgcgt aagcgctcac
     4621 gccgagccag gcgtgggcaa cgccgattcc ctggctgatc cggttgtcga acgcgtcgtt
     4681 ggcgatcggc gcaatgctgt cggccaccag tatcttcttg gccaggccga tcgtcagaaa
     4741 cgtcagtccg atcgcaaacg gctccagtcg cggacggtag ttctcgggaa gccggaactg
     4801 cggcatcatc tccgcgtgat gcagcaccgg gcctgcgatc aggtgcggaa agtacgtaac
     4861 gaacagcaga tagtgaatcg gattgcgctc ctgcaccttg cccgcgtaga cgtccgccag
     4921 gtacgcgatc tgggtaaacg taaagaacga aatacccagc ggaagaatca cgtccaggat
     4981 gggtatgtgc gtggaggcca cgtagttgac gctctgtagc aggaagttcg catatttgaa
     5041 atacgccagc gtgcacaggt tcgccgccac ggcgacgatc agaccgagtc ggcgcgtgcc
     5101 acccgaagac cgaccgatca gggtgccaaa ggcatagttg acgatgaccg atgccagcag
     5161 cagcaaggta taggccggca tccagtagcc gtagaaaaaa agcgacgcca gcgcgagcca
     5221 aaatgccgcc gtcgagcgac cgaacagacg agcgcagata aagaacaccg cgagcgtgat
     5281 cggcaaatac aggaacagga agacccctgt cgtaaacagc attgtcggtt tggaagatcg
     5341 tcgttataag ggagcgaatt cagtagtcat cttaacgaat tggattttat acaaatgtga
     5401 cgtttcactt aaggctaccc gttgcagcca agaagcagct gattgcagga tattgttaga
     5461 ttcgccttga ccgtcttgtc attcgcctga cgatttagaa ttcgcttctc gcggtacctt
     5521 cgcactggta accttcaggc cccgcgctcc cgcgctcccg cgctaccgcg ctaccgcgcc
     5581 agagccaaga gactagggag ggagagttcg ccagggatgg cgacttttcc gtttcgtgcc
     5641 gaacgtcccg atccaccctc ggtttgattc gatacagcgc ttcaatgcac gatggcgcta
     5701 tgaagatttt tgacgccgac gatcgggaaa tgatgcgtat cacctcggta tcacgcgagg
     5761 gttcgcagct ggtgattcag gggatgatct tcggctcgat gccgctgacc gcgaaattgc
     5821 gcccggaaga ggcgcgcgca gcattcaggc tgatggactt gcggacgacc tggtttctgc
     5881 tcaccctgct ccttcgtcgc cggcgcgact gatcaccgct tagaacattg cctcggcatc
     5941 gctgcctgac ggtaccgcct gctccaccac ccagtgttcc acgatcctgg cgttgaccag
     6001 gcgatagatg tccacccgca ccaagccgcg cgtcgcgccg ggcttctgat cccagaaggc
     6061 gtgcactgag accagatcgc gctgggccac ggtgcgtttc acgaccacgc gtgaatcggg
     6121 atattcattg gtaagttggt cgaccagtcc gcgcgcggcc tcgtcctggc ccccgggaaa
     6181 cgccggatcg tgttccttgt actccggcgc cacgtagcga ctgaaaccct cgcgcacatg
     6241 atgtttgctg aacaccgtct cctcgaacgc cagcacgatg tgtgtgttgg tacgggcgtt
     6301 ctctgcctcg atcacgaaac gtggggtgga acaaccactg acggccgcag ccgtcaggat
     6361 cagccacaga actctggcgc gcgtcatcaa ggcaccagaa cgctcgagcc gaacgtgcgc
     6421 cggctttcca ggtcgcgctg cgcctgggcc gcctccgcga gcgcatagcg ctgaccgatg
     6481 tgcacgcgca agctcttgcg acgcatcagc gcgaacagtt cgcgggacgc cgtctgacgc
     6541 gtttccgcag tcgccacata gtccgccatc cggctgcgag tgaagaacag cgaaccgcgc
     6601 ttggcgagtt ccgagggtgc gaggggtgcg gccggaccgg atgcgttgcc aaaactcacc
     6661 agcattccac gaggtttgag gctatcgagc gatgcctgtt gtgtatccct gccgacaccg
     6721 tcgtaaacca ccgccacacc ggcgccccga ttgagcttgc gaaccctggc cgccatgtcc
     6781 tcatagccta cgagcacctg gtgacagccg ttgcgtttgg cgagtgtcgc cttgtctggt
     6841 gaacccacca cgccgatcac cttcgcgccc agcgagcgcg cccactggca cagaatcagg
     6901 cccacacccc ctgcggccgc gggcaccagc accacatcac cacgtcctac cttatacgtc
     6961 tgccgcagca ggaaccagca ggtcatgccc ttcagcagca ccgcggcgac ctgctcggca
     7021 tccacgccgg ccgggatcct gatcagcgca ccggccggca atacccgatg ctccgagtag
     7081 gccccagcga ccggatacga atacgccacg cgatccccgg gtttgaatcc gcgcaccttg
     7141 cggccgattg cctcgatcac gcctgccgcc tccgaaccca ttcccgacgg cagcggctgc
     7201 gggtagagac caattcgtaa ataaacatcc gcgaagttga tgccgatcgc agtgtggcgc
     7261 accagtacct cctgttcgcc aggagtaccc acctcgacct gctcgcagcg caaaacctcc
     7321 gggccaccgt gctgatagat tcgaatcgct ctggccatca tgcgtcataa ttccctagta
     7381 aaagaatatc tgttcgctgc catcggccgg cggccttgga ttgcgcaacg aattttagcg
     7441 cctcttcgca ctcgagcgca ttgcgccccc gcgccccgcg tgcgataaag ccgccatgcc
     7501 caggccccag ctccgcaagc gtccccgtgc tgtcggcgtt tcggggcgta ctcccagaat
     7561 cgccacccgc aaacgccgcg ggatcggccg cccggcagcc ggctcacaga ccgtcggccg
     7621 cgacgcgctg atcgcccgca cctgcgaaat gctgaccgag ctgcctccga accggatcac
     7681 tcgggcggaa gtcgcgcggc acatgaatgt cgatccgtcg ctgattcgct actactttcg
     7741 cgaccgatcg acgttgctgg tggcggccgc cgagcgcctg atcgagacct ttcagcgact
     7801 gctggacgag gagcttgccc agtcggacat gtcagccgcc ggtcggctgc gtgcgcgcat
     7861 ctcggcgttg ctgaagctga acatcaacta tccgtttttt caccgcctga tgatcgatga
     7921 gctggtcaag atcgagtccc ggccggccca gcgctttctc gaggagctca cccacaaagg
     7981 ggtagtcggc tatggcgcaa tcgtcgatgc gggtgtcaag gagggctcgt tccgacgggt
     8041 caacagcgcc ttcctgttcc tggcggtgat cggtgtgtgc gagttcttcg tcaacggcat
     8101 gccgatcctg cgcatcgcga tgggcaagga atttgacgag cgagccattg cggcccgcta
     8161 tcgcgacttc atttgcgaac tgttgaccac cggcctcaaa tccaagtagg cgtcgaggcc
     8221 gaacctccgg tcatagaatt cccgaccggc ggaattacag atctggaggg agctgagcga
     8281 tgagtaagac aacgggcgat cgcctgccgc cgattccgga tgaccaacag acacccgcgc
     8341 agaggaaggt cgtgaaagca ttgctcgccg gttcacgcaa ggcggtgcgt ggggcgttca
     8401 tcgcgttgct gcgcagcccc gacctgtgtg accgcacgca gaagctgggc gagtacctgc
     8461 gctatcgcag cgtggttccg atcaagctgc gtgaactggc gatcctggct accgcccgtc
     8521 attggcagca gacctatgag tggcaggccc atgccacatc ggccgtcgag gcgggcctgc
     8581 ccgagcaggt gatcgatacg ctggcgctcg agtgggacgg cgcgggtctg gcgcccgacg
     8641 agcacaccgt gctccggttt tgccgtgaac tgcatcgcga ccgggtggtc agcgatcgca
     8701 cctacgatgc ggcgaagacg ctgctgggcg agaagggcgt tgtggagctg tgcggtattt
     8761 gcggctacta cgccatgctg gccatggtca tgaacgtggc acataccccg ccggtgtccg
     8821 aagggccgcc cgtgccgttc tcccctcctg aggcaaaaac tgcttaaaag ccctgggaag
     8881 ccaagcaaag cttacagcgc gggccaggcg gcccggcctt aaggcgttgg tgctcaaggt
     8941 tttaggttcc gggcgtgccc gaactcgtgt cggcagcctc ctacaagttc tcgcaagctc
     9001 ctgacttcgc agggcacccc cactccgcgg gcggtcgcaa gatgactacc gggtgttgcg
     9061 tggcatagac actgctcttt atcgttcact ggcccgcagc aggtagacga aattcttgac
     9121 ggttatccct ccctgtcgag ccgtcatact acttgtactg cgtcattagc cgcatcgtgg
     9181 ccactcccaa aacgatgcgg ctattttttt gtgccgtagg aatcgacgtg cagcccgggt
     9241 cttagggtgc acctacggcg gtgttccact ccaacgttac tgaacgactg aggagcgctt
     9301 cacagacctt gaacaatgaa tgctcggtag tccgcccctt tctttcggtg tggaatagcc
     9361 gcttgataac ccggactgtt gtaccaggcc ttagcatcgt cgactgtcgg gaattgcacc
     9421 atgaccattc cgtccggcgc cttgccttcc a
//
LOCUS       DATMRM010000348         9363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2048978, whole genome shotgun sequence.
ACCESSION   DATMRM010000348 DATMRM010000000
VERSION     DATMRM010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9363
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048978"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VNZ06_10345"
     CDS             <1..657
                     /locus_tag="VNZ06_10345"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW21194.1"
                     /translation="SVRGRDLGSTVAEVQDRIAHNIKIPTGYQIDWAGEFEDLKLAQQ
                     RLAVVVPIALLLILVLLYALFNSFKDSLFALAGIPFAAAGGIIALLIAGLPFSISAAI
                     GFVSLLGVSVMHGILMFTYYNTLRMDGMRSGEAIYHAAERRMRPMLMTALSACIGLLP
                     AALSHGIGSQVQRPLATVVVGGMLIGPMMLLVVVPALRMLISRKYDDTAPVSVSDLGH
                     "
     gene            1028..1192
                     /locus_tag="VNZ06_10350"
     CDS             1028..1192
                     /locus_tag="VNZ06_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21195.1"
                     /translation="MTATTLAVNVKRPVTGNGVLGGPHASAGGMIGGPAGNRNGIKAG
                     IDGTAFRHRK"
     gene            complement(1230..1835)
                     /locus_tag="VNZ06_10355"
     CDS             complement(1230..1835)
                     /locus_tag="VNZ06_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWW21196.1"
                     /translation="MEATRKRILCIEDDCETAALIAEELNDRNFAVSLAYNGRDGLDA
                     ILRCAPDLVLADVSMPVMSGFDLLERVTALEPRFCSMPFVFLTALADHDNELRGWQLG
                     ADDYVTKPVDFDLLAAVIASRLKRVARSTLWPRRIDLGERERETLTWAARGKTFAEIG
                     EILGVSRRTVEFHLDNARRKLGVPTRTQALIKAAVGELIEL"
     gene            2051..3646
                     /locus_tag="VNZ06_10360"
     CDS             2051..3646
                     /locus_tag="VNZ06_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW21197.1"
                     /translation="MKRTRSITIRLSLVFLFLFLVAAMLGAFGIGSLSYFNDVSSQVR
                     DRWLPSTGVLGDLNNHTSDFRGIEATTVLASTVTERAASDQEMAQLDRNIAASKDSYL
                     RLSHGADELSLYETFAVSWDQYRLSAAAARELARRGDIAGAIARYNSTSKAAYDVASD
                     ALDALTAHTLASARRASLRESQVYDRAWWLIVVTIGMTGLCVAGAMSYVRRSISAPIV
                     ALAVRMHRLAANDTGIEVHSTERYDEIGEMARAVVVFRNNAIELMNSRIGLERQATML
                     REKLAEEQRLMLLQRNFVSMASHEFRTPITIIDAHAQRLISMKERLSWEDVGERARKI
                     RNSARRMTHLIENLIDSMRVIDRDVRLYVHPTSVDVPALLHEVCQLQREIAPQAQILE
                     DFRAHRPELLGDASLLFQVFSNLLSNAIKYSPGGGLIAVEVVGEDRWVAVSVLDRGIG
                     IPDRDRAGLFERYHRGSNAGGIVGSGLGLYFVKTVVELHGGDVSAQPREGGGSCLRVR
                     LPRDSSLGQRLSFPKAPDAVPVA"
     gene            3898..5685
                     /locus_tag="VNZ06_10365"
     CDS             3898..5685
                     /locus_tag="VNZ06_10365"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWW21198.1"
                     /translation="MVTDSAKLPVAHATITAVHSGDGAIRATLSGSDGVYSFADLAPG
                     KWSVTTHVDGVPDVTGEALEVVARQATRFDIVTNAKGGAAPAVANAVAGNAPGGTDLS
                     ASALARAIAAVVPEALQAPDPAPPGVDTHTPFGQDDDIGWLNGQGREKSPIFDTKFFT
                     PEIRFDVDYMQDFNHPRDHTIDGSTEAFRSQEFQIEQVSFGGNFHWNNVQARFLSIAG
                     LFAVTTPRNDASYGVGQWDLQAAYKYFSEANAGYHFDNVGHGLNIDAGIFVSYIGLFS
                     YYNYDNWTYQPSFVSSNTPWFFNGLRIQYWPTQNLKIEPWLINGWQSYAMFNGHPGFG
                     GQILWIPNNNFKLVLNTYTLGQDNLHGIFQNCNGSNLNAPNLLNGNAPSAAQCANAPY
                     INAAKIQRIHEDDSVLYRYYQADGQQGNGISKAAFSLTWDIGCQYGGGVSCDKGPNKD
                     QFIGVMAYNRLWFDHDRFAVTTGGGFMNNPGRYLALLPPINGAVASLGTPYWTENPGQ
                     KLFQWDSQVNLQYMPKDWITWWTELTYRHSDVPYWTGENGITPPGAINGTQAGTVPGG
                     LATNTDGSQFYPDLRTREVIWGAGVLVKF"
     gene            complement(5740..6474)
                     /locus_tag="VNZ06_10370"
     CDS             complement(5740..6474)
                     /locus_tag="VNZ06_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW21199.1"
                     /translation="MTLGMHLALVVEDDRAVQKVLRMMLESNGFRVVIAENCDRGQRD
                     ARSQRPDIAIVDLGLPDQDGIEVIRSVRTWSAMPLIVLSARTAEAQRLAAFEAGADDY
                     VIKPFSAPELLARVRAALRRHSRGEIPSAKLELGDISIDLSLRVVHQRGGAEVRLTPL
                     EHRILETLARNRDRVVTHSQLMKEVWGPNRADTRSLRVFITTLRKKLELDPTRPAYIV
                     TEPGVGYRLVVDSNLGEGASGTQAGD"
     gene            complement(6471..8501)
                     /locus_tag="VNZ06_10375"
     CDS             complement(6471..8501)
                     /locus_tag="VNZ06_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HWW21200.1"
                     /translation="MDVTSKYHVASASAANAENRPSRPECLLVVLGSSTYDLDLIHAG
                     KQLAEALYAPWIVVCVQSPGLRILPDHERDRRALVGIAEALGAEAATLHGMSAVKTIA
                     AYARLRQATKILLGSPTRLGWHTLVQHFRIAALKRLTTNIEVMAVATQARRALRPAGT
                     APARVPARTHSRSNWGHYVWAITITALCTIFAFPLAGHVDLINLVMIYLLGAAAAGLM
                     LGRGPATLTAVTNTLAFDYFFVPPIFSFFVIDSTYIVTFSAMLLVALIIANLIIALRE
                     QTGAASAREQHTAALYAITRDLSVAREATAMVSLAVRHITEVLGIHARVLLCDESGQL
                     TDVSSAAASELPPAVNPTTAQWVAIRCERAGSGTRQFSDERSRYLPLRGSRVTIGVLV
                     VERADPADVMLPEQLRLLDAIADQLALALERARLAEVAHAANLAAERAAMRNTLLASI
                     SHDLRTPLSAIAGAGSIVAQSNFALDVYRRVTLGRLIEDKARDMTDLLTNVLELVRLE
                     SGADVLNKDWHSLSDLIGQAITRHESALTGWPIKTDLPADLPLLYVDSGLIVQLFSNL
                     LDNVTKYTPRDTRICLSASRDADLIRLVVEDSGPGLGIDAPDHLFEKFARGRLEGNHG
                     GMGLGLAICRAVARLHGGEIRAAVSALGGARFVITLPHVEEPSPIEAPPSIV"
     gene            8702..>9363
                     /locus_tag="VNZ06_10380"
     CDS             8702..>9363
                     /locus_tag="VNZ06_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HWW21201.1"
                     /translation="MSGTEISLMATVPRGLSDLLGRELQSFGALEIRERATGVSFRGT
                     LETAYRACLWSRVASRVLLQLAEFNAHDADEFHSRVRAFDWGAHIDHARTIACEFTGK
                     HPGITNSHFGALRLKDGICDRLRDDTGSRPDVALVRPAVRIRAHANGPQISLSLDLAG
                     EGLHRRGYRTDAGEAPLRENLAAGILLRARWDEAAQRGVEFLDPMCGAGTLVIEAAMI
                     AAR"
BASE COUNT         1811 a         2803 c         2950 g         1799 t
ORIGIN      
        1 agcgtccgcg gccgcgacct cggcagtacg gtggcggagg tgcaggaccg aattgcgcac
       61 aacatcaaga taccgacggg ctatcagatc gactgggcag gcgaatttga agatctgaag
      121 ctcgcgcagc agcggcttgc agttgtcgtg ccgattgcgc tgctgttgat cctggtattg
      181 ctgtacgcct tgttcaattc attcaaggac agcctgtttg cactggcggg catcccattc
      241 gccgctgctg gcggcatcat cgcactgctg atcgccgggc tgcccttcag catttcggca
      301 gcgatcggct tcgtgtcgct actcggtgtg tcggtgatgc acggcatcct gatgttcacg
      361 tactacaaca ccctgcggat ggatggcatg cgttccggag aggcgatcta tcacgctgcg
      421 gaacgcagaa tgcggccgat gctgatgacg gcattgtcgg cctgtattgg cttgctaccc
      481 gccgccctgt cgcacggcat cggtagccag gttcagcggc cgctcgcgac ggtcgtggtg
      541 ggtgggatgc tgataggacc gatgatgcta ttggtggtcg ttccggcgct gcgcatgctg
      601 atctcgcgca agtacgacga cacggctccc gtgtcggtga gcgatctggg gcattgatgc
      661 aatgagaaac gcagtgctta tgtggcttgc ctgcagcgca ctgctcgtga gctgtctggt
      721 gagcccatgg gctgccggca gcgagccggt gagctctggc gagcaggtca ctgcggccgc
      781 cgtgcgaacc aatgataggc cagggtccgg tcgtgcaaat gccgtccgcg gcatcgcgcc
      841 gcgggcgatg gggcatcaaa ctgcgcagcg caatccgcct cgcacccaga gggcggtctc
      901 gggctcccat gccttctcgg caaacccagc agcctcttcc ccggcgagcg gcaatgcatg
      961 gcgcaggtcg ggggtgactc agcttgcacg tcgtcccggg ccagcggcag cagcactgcc
     1021 gcgacggatg accgccacta cgctggcagt gaacgtcaag cggcccgtga ccggcaacgg
     1081 cgtgctcggc ggaccgcacg cctcagctgg cggcatgatc ggcgggccag caggcaacag
     1141 aaacgggatc aaggccggca tcgatggcac cgcatttcgc catcgaaagt gaggcgcgga
     1201 ttgtgctcgg cctgtccgcc gtcggtgggc tagagctcga tcagttcgcc gacggcggcc
     1261 ttgatgagcg cttgtgtgcg cgtcggtacg ccaagtttgc gacgcgcatt gtccagatga
     1321 aattcgacgg tgcgacgact gaccccgagg atctcgccga tctcggcgaa agtcttaccg
     1381 cgagcagccc acgtgagtgt ttcgcgctca cgctcaccca gatcgatccg tcgcggccag
     1441 agggtgctgc gcgccacacg cttgagccgc gacgcgatca ccgccgcaag cagatcgaag
     1501 tccaccggct tggtgacgta atcgtcagcg ccaagctgcc agccacgcag ctcgttgtcg
     1561 tgatcggcca gagcagtcag aaacacgaat ggcatgctgc agaagcgcgg ctccagtgcg
     1621 gtcacgcgct cgagcagatc gaagcccgac atcacgggca tgctgacgtc ggccagtacc
     1681 aggtccggcg cacagcgcag tatggcgtcg aggccatcac gaccgttgta cgcaaggctg
     1741 accgcgaagt tgcgatcgtt gagctcttcg gcgatcaggg ccgcggtctc acaatcgtct
     1801 tcgatgcaga ggattcgctt gcgggttgct tccattgtct tgctaacggc gacaggctcc
     1861 gcgttacggc gtgccgtcct ggattgaact gacactagca tcgacagtgt taagagaagg
     1921 taaaaaaacg tcccgacgct tccatcgtcc gcacgcgcgg cagatagtgc gctcaggctg
     1981 ccttggcaat ggccactgca gtgcgctcaa ttctgtacga ctggaatcgg tgctcgactg
     2041 cgatatgccc atgaagcgaa cacgctcgat cacgatccgc ctgtcgctgg tgttcttgtt
     2101 cctgtttctc gtcgcggcga tgctgggtgc ctttgggatc ggcagcttga gctatttcaa
     2161 cgatgtgtcg tcccaagtgc gcgatcgctg gctgcccagc actggcgttc tgggagatct
     2221 gaacaatcac acctcggatt tccgcggcat cgaggccacg accgtactcg cgtccaccgt
     2281 cacggagcgt gcggccagcg atcaggagat ggcgcagctc gatcgcaaca tcgccgcgtc
     2341 gaaggatagt tatctgcggc tctcgcatgg cgcggatgaa ctctcgctgt acgagacgtt
     2401 tgcggtgagc tgggatcagt atcgcctgtc ggcggcagcg gcacgagaac ttgcgaggcg
     2461 cggagatatc gccggtgcca ttgcgcgcta caattctact tcgaaggccg cctacgatgt
     2521 ggccagtgac gcgctggacg cactaacggc gcacaccctg gcgagcgccc gacgagcgag
     2581 cctgcgcgag agccaggtct atgatcgcgc gtggtggctg atcgtagtta ccatcggcat
     2641 gacgggccta tgtgtggcgg gggccatgag ctacgtccgg cgctcgattt ccgcgcccat
     2701 tgtggctctt gcggtgcgca tgcatcggct tgccgccaac gacaccggca tcgaagtgca
     2761 cagcaccgag cgctacgatg aaatcggcga gatggcacgt gcggtcgtgg tgtttcgcaa
     2821 caacgccatc gagctgatga atagtcgcat tggactcgag cggcaggcga cgatgctgcg
     2881 cgaaaagctg gccgaggaac agcgcttgat gctgttacag cgaaatttcg tgtccatggc
     2941 ctcacacgag tttcgtacgc cgattacgat tatcgatgct cacgcccagc ggctcatctc
     3001 aatgaaggag cgattgagct gggaggatgt gggtgagcgc gcacgcaaga tccggaactc
     3061 agcgcgacgc atgacacact tgatcgaaaa tctgatcgat tcgatgcgag tcattgatcg
     3121 tgacgtgcgc ctgtatgttc accctacgag cgttgacgtg ccggcgctgt tgcatgaggt
     3181 ctgccaacta caacgagaga tcgcacccca ggcacagatt ctggaggatt ttcgggcgca
     3241 tcggccggaa ctgcttggtg atgcgagcct gctgtttcaa gtgtttagca accttctgtc
     3301 gaatgcgatc aagtattccc caggcggtgg actgatcgcg gttgaggtgg tcggggagga
     3361 ccgctgggtc gcggtatccg ttctggaccg cggaattggc atcccggacc gcgaccgcgc
     3421 gggattgttc gaacgctacc accggggcag caatgccgga gggatcgtgg gaagcggcct
     3481 tgggctgtac ttcgtcaaga ctgtcgtgga gctgcacggt ggcgacgtgt ctgcgcagcc
     3541 ccgcgaaggg ggcggatcct gcctgcgcgt gcgattgcca cgcgactcat cactcggaca
     3601 gaggctgtcg tttccgaaag cgccggacgc ggttcccgtc gcataagctc gccgttaaga
     3661 aacattttct taacaacttt cggtctaggc gcgaccgagt cgcatcgcct ataagaacta
     3721 aacatcgcgc gacgggcggt cgtgtgcgaa taacgacgta tcgaaccaag tctcgaacgg
     3781 aagggatcaa tgatgatagg aaactggctg catcgaactc tggtgtgtgc tatggcgaca
     3841 gcatccttca ttgcggttgg gcaggccgca gactcgtcgc cgggaaccat tgtgggcatg
     3901 gtcacggatt cggcgaagct tccagtggca catgcaacga taacggcagt gcacagtggc
     3961 gacggcgcca tccgtgcgac attgtctggt agcgacggtg tctactcatt tgcagatctc
     4021 gctccgggca agtggtcagt gaccacgcac gtcgatggcg ttccggacgt gacaggggag
     4081 gcgcttgaag tagtcgcgcg ccaggccacc cgctttgaca tcgtgaccaa tgcgaaaggg
     4141 ggtgcagcgc cggccgtcgc caacgcggtc gcgggcaatg ctcctggagg caccgacctt
     4201 tccgcgtcag cgcttgcgcg ggcgatcgcg gcggtcgtgc ctgaggccct gcaggcgccg
     4261 gaccccgcgc cgcccggggt tgacactcac actccgtttg gccaggacga cgacataggc
     4321 tggctgaacg gccaggggcg tgagaaatcg ccgatatttg acaccaagtt ctttactcct
     4381 gaaatccgtt tcgacgttga ctacatgcag gacttcaatc acccgcgcga tcatacgatc
     4441 gacggatcga ctgaggcatt ccgatcgcag gaatttcaga tcgagcaggt cagcttcggc
     4501 ggtaactttc actggaacaa cgtgcaggca cgcttcctgt cgatcgcggg tctgtttgcc
     4561 gtgaccacgc cccgcaacga cgccagttat ggcgtgggtc agtgggacct gcaggccgcc
     4621 tacaaatatt tctcagaggc caatgccggc tatcactttg acaatgttgg acatggcctc
     4681 aatatcgacg cgggcatctt cgtctcttat attggtctgt tcagctacta caactacgac
     4741 aactggacgt accagccgtc gttcgtatca tccaacactc cctggttctt caatgggctg
     4801 agaatccagt attggccgac gcagaacctc aagatcgagc cgtggctcat caatggctgg
     4861 cagtcctacg cgatgttcaa cggccatcct ggctttggcg gacagatcct ctggatcccg
     4921 aacaacaatt tcaagttggt tttgaacacc tatacgctgg gtcaggacaa cctgcatggc
     4981 atcttccaga actgcaatgg atcgaacctc aacgctccga atctcctgaa cgggaacgct
     5041 ccgagtgccg ctcaatgtgc caatgcgccg tacatcaatg ccgccaaaat acagcgcatt
     5101 cacgaggacg acagcgtcct atatcggtac taccaagccg atgggcaaca gggtaacggt
     5161 atatccaagg cggcgttctc cctgacctgg gacattggct gccagtacgg tggtggcgtc
     5221 agctgcgaca agggaccaaa caaggaccag ttcattggtg tgatggcgta caaccgtcta
     5281 tggtttgatc atgaccggtt cgccgtcacc actggcggag gcttcatgaa caatcccggc
     5341 cgctacctgg cgctattgcc accaatcaat ggagccgtcg cttctctagg aacgccgtat
     5401 tggacggaga atccggggca gaagctgttc caatgggatt cgcaggtcaa tctccagtac
     5461 atgccgaagg actggattac ctggtggacg gagctcacgt accgccattc agacgtcccc
     5521 tactggactg gagagaacgg aatcacccct ccgggcgcca tcaatggcac tcaggcgggg
     5581 acagtgcccg ggggactggc caccaacacc gacggcagcc agttctaccc cgatcttcgc
     5641 acccgcgaag tcatttgggg tgcgggggtt ttggtgaagt tctagctgcc tccggggacg
     5701 acgggcgagg ggggcacgcc tcgccccgac agtcctcggc taatcgccgg cttgagtccc
     5761 gctggcgcct tcacccagat tggagtccac caccaggcga tatccgaccc cgggctctgt
     5821 cacgatatag gccggacgcg tcggatcaag ttcgagtttc ttgcgcaacg tcgtgatgaa
     5881 tacccgcagc gatcgcgtgt ctgcgcgatt gggtccccat acctctttca tcaactgaga
     5941 atgcgtgacc acgcgatcac gatttcgagc cagggtttcc aggattcgat gctcgagcgg
     6001 tgtgagcctc acctcagcac cgcctcgctg gtgcacgacg cgcagcgaga ggtcgattga
     6061 gatatcgccc aattcgagct tggcggatgg gatttcaccg cgcgaatgtc gtcgcagcgc
     6121 cgcgcgcacg cgagccagca gctcgggtgc gctgaagggt ttgatcacat agtcgtcggc
     6181 acctgcttcg aaggcggcca gcctctgggc ctcggcggtg cgagctgata acacgatcag
     6241 cggcattgcc gaccaggtgc gcacgcttct gatgacttca atgccatcct ggtcaggcag
     6301 acccagatcc acgatggcga tgtcggggcg ctgcgagcgc gcgtcgcgct ggccgcgatc
     6361 gcagttctcg gcgatgacga cccgaaagcc gttggactcg agcatcatcc gcagcacttt
     6421 ttgaacggcg cgatcatctt cgaccaccag cgcgagatgc atcccgagcg tcatacgatt
     6481 gatggcggcg cttcgatcgg ggacggctcc tcgacatggg gtagggtaat cacgaatcgg
     6541 gcgccgccca aggcactgac ggcagcgcgt atctcgccac catgtaggcg cgcgaccgcg
     6601 cgacagatcg ccagtccgag acccataccg ccgtgattgc cttccaggcg accgcgggcg
     6661 aacttttcga acagatggtc cggtgcgtcg attcccaggc caggccccga gtcctcgacc
     6721 accaggcgga tcaggtcagc atcgcggctg gcggacagac agatacgcgt gtcgcgcgga
     6781 gtgtatttcg tcacgttatc gagcagattg ctgaacagct gcacgatcag acccgaatcg
     6841 acatatagga gtggcaaatc cgctggcagg tcggtcttga tcggccagcc cgtcagagcg
     6901 ctctcgtgac gcgtgatggc ctgtccgatc aagtcgctca gcgagtgcca gtctttgttg
     6961 agcacgtccg cgcctgactc caatcgcacc agctccagca cattggtaag caggtccgtc
     7021 atgtcgcggg ctttgtcttc gatcaatcgg cccagcgtca ctcgccgata gacatccagc
     7081 gcaaagttgc tctgggcgac gatactgcct gcccccgcga tcgcggacag cggggttcgg
     7141 aggtcgtgtg aaatggaggc cagcagagtg ttgcgcatgg ccgcacgctc ggcggcaagg
     7201 ttcgcggcat gcgccacctc ggccagacga gcgcgctcga gcgctagcgc gagctggtcg
     7261 gcgatggcat ccagcaggcg cagttgctcc ggtagcatca catcagccgg atcggcacgc
     7321 tcgacgacca agaccccgat cgtgacacgg gagcctcgca gcggcagata tctggagcgc
     7381 tcatcggaaa actgtctcgt ccctgagccg gcgcgctcgc atctgatcgc tacccactgt
     7441 gccgtggtcg gattgaccgc aggcggtagc tcggatgcag ctgcagaaga cacgtcagtg
     7501 agctggccgg actcatcgca cagtagtacc cgcgcatgta tgcccagaac ctcggtgata
     7561 tgacgcaccg ccagagacac catcgccgtc gcctcgcgtg caaccgaaag atcacgggtg
     7621 atcgcgtata gcgctgccgt atgttgctcg cgggcgctcg ccgctccggt ctgctcccgc
     7681 agggcaatga tcagattggc gatgatgagg gccaccagca gcatcgcgga gaatgtgacg
     7741 atataggtcg agtcgatgac gaaaaaactg aaaattggcg gcacaaagaa atagtcaaac
     7801 gccaaggtgt tggtgacggc cgtcaatgtt gctggtccac gtcccagcat caatccggct
     7861 gcggccgccc ccaatagata gatcatcacg aggttgatga ggtcaacgtg tcccgcgagc
     7921 ggaaacgcga agatggtgca cagagccgtg atggtgatag cccagacgta gtggccccag
     7981 ttcgagcgcg aatgtgtccg ggcaggcacg cgcgccggcg cagtcccggc gggccgcagc
     8041 gcgcggcgag cctgcgttgc cacggccatg acctcgatat tggtggtcag tcgtttgagg
     8101 gctgcaatgc gaaaatgctg gaccagcgtg tgccatccaa ggcgggtcgg tgatccaagc
     8161 aggatcttgg tagcctgacg caagcgtgca taggccgcga tggtcttgac ggctgacatg
     8221 ccgtgtagag tcgccgcctc ggcgcccagc gcttcggcga tgcctacaag tgcgcggcgg
     8281 tcacgttcgt gatccggcaa gatacgcagg cccggactct gaacgcagac gacgatccat
     8341 ggtgcgtaca atgcctccgc gagctgcttg ccggcgtgga tcaggtccag atcgtatgtg
     8401 ctcgagccaa ggacgaccag cagacactca ggcctgctcg ggcggttttc ggcgttcgcg
     8461 gcagatgcag acgcgacgtg gtacttggaa gtgacgtcca tgttcaattg gggatcgggg
     8521 cgcggcgtcc agtgcagcgt acgagactcg ccattagttc gacgttaaga aatgctttct
     8581 cttgccgcca gcgcttgcac agtctgtcta aaaccgatca ggtgtagtgc aattcattga
     8641 taacgaaaga aaataggcga gtcagagaca tctgccctca tctggaagct ggcgcagggc
     8701 aatgagcggt acggaaatct cattgatggc gacggtaccg cgcggtctgt ccgatctgct
     8761 cggccgcgag ctgcaatcct tcggcgcgct cgagatccgc gagcgcgcca ccggcgtctc
     8821 gttccgcggc acgctcgaga cggcctaccg cgcgtgtctg tggtcacgcg tcgccagtcg
     8881 cgtgctgctg cagctggcgg agttcaatgc gcacgatgcg gatgaattcc actcccgcgt
     8941 gcgcgcattc gactggggtg cacacatcga tcacgcgcgc accatcgcgt gcgaattcac
     9001 cggcaagcat ccaggcatca ccaacagcca tttcggcgct ctgcgcctca aggatggcat
     9061 ctgcgatcgg ctgcgcgacg acaccggctc gcgtcctgat gtggcgctgg tgcgcccggc
     9121 cgtgcgcatc cgcgcacatg ccaatggtcc gcaaatcagc ctgtcgctgg atctggctgg
     9181 cgagggactg caccggcgcg ggtatcgcac cgacgccggc gaggcgccgc tgcgcgagaa
     9241 tcttgctgcc gggatcctgc tgcgcgctcg ctgggacgag gccgcgcagc gaggtgtgga
     9301 attcctggat ccgatgtgcg gcgccggcac tctcgtgatc gaagccgcca tgatcgccgc
     9361 gcg
//
LOCUS       DATMRM010000349        23817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4557444, whole genome shotgun sequence.
ACCESSION   DATMRM010000349 DATMRM010000000
VERSION     DATMRM010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 23817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23817
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4557444"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VNZ06_10385"
     CDS             <1..547
                     /locus_tag="VNZ06_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWW21202.1"
                     /translation="EHKSPQMLALNFRGRLPVLRDNDYVVFESLGILYYLDRKYPEPP
                     IFGRTPEESGVIMRVICEYQSYAEPPLKQITTALQQRHDRKVDETIAEAMHRVAGEAR
                     TIEQRLARSDWVVGESFSAADMMIYPDIRLLVRMLQKPQAHELAARFLPMEVNYPAIG
                     RWLDRVAALPGHERTWPPHWR"
     gene            674..2092
                     /locus_tag="VNZ06_10390"
     CDS             674..2092
                     /locus_tag="VNZ06_10390"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HWW21203.1"
                     /translation="MSQDKVLVDFPGRIVVVGFGSIGQGVLPLILRHIGVTAERITIV
                     TAEDVGADEAARYGVKFIKQRLTRENFRRTLDPILGRGDFLLNVSVDVSSIALIRYSW
                     DKGAMYLDTCIEPWPGGYTDPTIPAARRTNYALREEALALRSGSARAPTAVLTHGANP
                     GLVSHLVKQALLNVAADVGIGNVSPTSRAEWADLAQRVGLKVIHVAERDTQVSTIPKE
                     PNEFVNTWSVDGFVSEGSQPAELGWGSHERNFPRDGRRFDFGGGSAIYLGQPGVTTRV
                     RSWTPNYGSYHGFLITHGEAISIADYLTVRKDAQVLYRPTVHYAYHPSDAAVLSVHEF
                     CGSNYQIQNRKRILMDDISKGTDELGVLLAGHKKNAYWYGSQLTVDQARALAPYNNAT
                     SLQVTAAVLAGMVWAMENPNNGLTEPEDMDYRRVLALCMPYLGKIVGEYSDWTPLTDR
                     ERLFPEDLDKSDPWQFKNVRVS"
     gene            complement(2109..2609)
                     /gene="greB"
                     /locus_tag="VNZ06_10395"
     CDS             complement(2109..2609)
                     /gene="greB"
                     /locus_tag="VNZ06_10395"
                     /inference="COORDINATES: protein motif:HMM:NF002506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HWW21204.1"
                     /translation="MGRFRAPSKPGSKYITPAGAARLRAELEELWRRERPQVTASVQA
                     AAAQGDRSENAEYTYGKRRLREIDRRVRFLRARLDGMVIVDHPPSDRQRVFFGARVTL
                     ETSVGEELRYRIVGPDEIDLAGDCISMDAPLGKALLGKRLDDEVSVELPSGSQRYVIV
                     AIDYPA"
     gene            2811..3374
                     /locus_tag="VNZ06_10400"
     CDS             2811..3374
                     /locus_tag="VNZ06_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004732432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWW21205.1"
                     /translation="MAVAMLSSGCGYNRIQQQDEAVKATWAEVVNQYQRRADLVPNLV
                     NTVKGFAQQEQKVLIGVTEARSKVGSIQATPELVNDPQAFQKFEAAQGQLTQALKSLI
                     AVSENYPQLKSDQNFRDLQSQLEGTENRITVARNRYIQAVQDYNINIRSFPTNLTAMM
                     FKYQVKQNFTVQNEAEISKAPTVDFSH"
     gene            3390..4256
                     /locus_tag="VNZ06_10405"
     CDS             3390..4256
                     /locus_tag="VNZ06_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HWW21206.1"
                     /translation="MRLALAALLLLLAAALPGARAQALQPAPKLETRVTDLTGTLTAG
                     QQAELEQKLRDFESRKGAQIAVLLVPTTHPEEIEQYSIRVVEAWRLGRKKVDDGALLI
                     IAKNDHSLRIENGYGLEGVLTDAMSNRIIEDTMLPLLRQDQYYAAISAGTDQMIKLID
                     GEALPPPDQTWRRPRPLPLNLVVGLFFTFFIVSTVLRSIFGRMAGSLLCAGGMGLLVF
                     GQTQVVVFAIGAAVLAFVLAMIGVGSGWSSGRYGGYGGFGGGGFGTGGSGGGGFSGGG
                     GSFGGGGASGRW"
     gene            4256..4756
                     /locus_tag="VNZ06_10410"
     CDS             4256..4756
                     /locus_tag="VNZ06_10410"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HWW21207.1"
                     /translation="MHLLLRLLRHLTTFALRTRMLFAPPVLGQIEHAVADAEQHHSGE
                     IRFAIETALPLPAVWQGVTPRERALQVFAQLHVWDTHANNGVLIYVLRADRAVEIIAD
                     RGICARVSEAEWQQLCRAVESDYRAQRYAQGSVAAVAGVASLLGRHFPGGGSAVNELP
                     NQPVLL"
     gene            4779..6011
                     /locus_tag="VNZ06_10415"
     CDS             4779..6011
                     /locus_tag="VNZ06_10415"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWW21208.1"
                     /translation="MPTTPWAVAVLTATTLGTLAGCSLFNGREVHAPAPRMQAPPPPP
                     PKPQFEAPIDTHTFTLTNPDDEVVGAVQITHASKEDTLPDIARRFNIGYEEIVRANPG
                     VDPWIPGDGRSIVVPTRFVLPDAPREGIVINVAAMRLYFYPPHKKGEPQVVYTYPIGI
                     GKVGWSTPMGVTKIVLHEKDPIWRPSAALRKDHFNDNGEDLPAVVGPGPDNPLGKYEM
                     RLGWPSYLIHGTNKPYGIGLRSSHGCMRLYPEDIEKLYFMVPDGMSVRVVNQPFLFGW
                     KDRQLYLQAYTVLEDDSRDWNHAQQKLLAHGLAQHTQKQLKDSGIQIDWDSVKAITHE
                     PRGIPVSVTGTDNNIDAVLAAAPTVENRIPEGADWDGGDASQDNGNSAKPLLQEREPA
                     ANAKGPVTVPTAKRSSGG"
     gene            6017..6175
                     /locus_tag="VNZ06_10420"
     CDS             6017..6175
                     /locus_tag="VNZ06_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21209.1"
                     /translation="MAQLSEWLQLMLAEIARKQDEAQRASEEQQQRRNEAPQPTAPSP
                     PKYLSTQS"
     gene            6245..7819
                     /locus_tag="VNZ06_10425"
     CDS             6245..7819
                     /locus_tag="VNZ06_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21210.1"
                     /translation="MADLLVSAADTVRLIRPQATIRRAPTAITAFVGRALKGPVNHAI
                     SIGSFNDYQRVFGGLWQPSTLSYAVEQYFENGGRAAIVVRVVNGGRAPTMRLPAGNGS
                     LNLIGLSPGTREFLRASVDYDGIQPHETDRFNLILQRIRAPGSEFIEDQEIFRRLSIL
                     PGADRSILDLLTDSRLARLHGPLPSQRPDRPRGLPPIAAVGYVMSNGDGDDGDALSNY
                     DIIGDAKSGSGLFALQGTEHFNFLCVPPLTRELDVGLPALLVALRMCRLHHAMLLVDP
                     PARWSDASEVLNDLRAWPFYSEDAAMFYPRVQAYDRLRGRQEIFGSAAAAAGLLARLD
                     RTSPIWSPTDTEEAQMRPALRPATMVADSDRDRLLHAGVNLLQPSRMPRTRIKLRTLI
                     PEAGVKNEWRYLCARRFALFVMSSIERGTRWVLFEQPGAALWERVRLEVTAFLESLED
                     EGAFAGRSAEENYFVVCDERVNDTEHVAAGRFQLVFGFADSRPGDFQTFLVSHQPGHS
                     SARPASVNRYALLPQS"
     gene            complement(7788..8294)
                     /locus_tag="VNZ06_10430"
     CDS             complement(7788..8294)
                     /locus_tag="VNZ06_10430"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HWW21211.1"
                     /translation="MRPQLDWLTTTQTAPVSLTMHRYQRMQRWPWGKWRFERAAGEHL
                     PMLRTLRAHLQELRVGLCRAMMNAHRRVRTIDGEIDPIAIAALAQLAASFVIEVSVPE
                     GLYWNSRGLTIEHLAQPGDAAVALARLDKIDWGHPGLVGVPVTVSDRDNKEVARAVVS
                     FAVAARND"
     gene            8468..8995
                     /locus_tag="VNZ06_10435"
     CDS             8468..8995
                     /locus_tag="VNZ06_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWW21212.1"
                     /translation="MANRYVVFSHGKDSGPWGKKITALADVARAEGYDVESVDYRGID
                     TPRGRIDKLIEVCKKLSGDLVLVGSSLGGYVTVSAASLLHARGAFIMAPALYVEGLPP
                     LRERLLDCPTMIVHGWNDEVIPVEHSVQFAREYRAALHLLDGDHQLHSQLRFIRYLFE
                     YFLISLDLPAELGAS"
     gene            9007..11436
                     /gene="plsB"
                     /locus_tag="VNZ06_10440"
     CDS             9007..11436
                     /gene="plsB"
                     /locus_tag="VNZ06_10440"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF003441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HWW21213.1"
                     /translation="MQGLLRRILALWVRATLRPEDAAARLNGRTVPICYVLERVSASD
                     LAVLQNLCVRDRLPRPGRRLLARQLRSARSSLALTRAVGFWRSRDDRRPPELLLNLIN
                     ALRTDLSLDVSLVPAAVYWGRAPQRERSLIRLLVSEDWVIASRVRRVITVIFNGRNTT
                     VQLGDPISLRSLLGTEADTRVSARRVSRLLRTQLSRLRTALIGPDLSHRRTIVAEVLR
                     TRAVRAAVAQEVREQDQSTRRSVLLEARRYANEIAANYSHTFVSLMAMGLAKLWNRLY
                     DGVEFEHLDRLEQLAQGNEIIYVPCHRSHMDYLLLSYAIYQHGYAVPHIAAGLNLNLP
                     IVGRFLRKGGAFFLRRSLRGSSLYTVVFMKYLATMMARGHSIEYFIEGGRSRTGRLRP
                     PMGGMLAMTVRSYLREPRRPLVFIPVYFGYERIVEGSTYVGELSGRPKEKESVLGLLR
                     MIGKLRQRFGRVHVNLGEPLYLDALIDQFRPGWRQEEFDDQNRAPWVRALVEELALRI
                     MRRINAAATVTPINLLAMALLGTQRQAMLETDLISQIEAMLAVLRAVPYSNLVSLSTQ
                     SAPEMIKYGLELNVITRENQRAGHIVRMSPEHAVLATYYRNNVLHLFAMPSLIACCFL
                     SNSMMRTEDVLRLASRIYPYIAAELFLRWSEDEVPAVVAELLAAMAQLGLLVREGDLW
                     HRPAPVSAAAMHLSLLAQSTIQTIERYYLAIALLIRSGSGAITQKLLEENCELMAQRM
                     TALYGLNSPEFFDPSLFESFIDLLRSRDVVRTDEAGRLTFDEVLLRVEADAQLVLSEQ
                     IRHSILQVTHS"
     gene            complement(11495..12025)
                     /locus_tag="VNZ06_10445"
     CDS             complement(11495..12025)
                     /locus_tag="VNZ06_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21214.1"
                     /translation="MTLVALWLPILLSAVIAFMAGWLMHMFVRHHRNDFKAVPNEPSA
                     CQSLGSLGLTPGDYFIPHASQSQRSSAPVTVIMTVMRPGGVQLGSALVQWFLYQLAIS
                     FLCAYVASRTLAFAAPYLNVFRVVGTVGFMAYSVAYAHESIWWQRGWGITVRYMFDGL
                     VYSLLTAGVFGWLWPR"
     gene            complement(12066..12593)
                     /locus_tag="VNZ06_10450"
     CDS             complement(12066..12593)
                     /locus_tag="VNZ06_10450"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HWW21215.1"
                     /translation="MRVYSFPAIVADDARILILGSMPGVASLEAHHYYAHPRNRFWSI
                     LERLCGITGPLRYDARLQLLRAHHLALWDVLHSCVRPGSLDAAIEHHSAKPNDLMGLL
                     RATPSIRRICCNGGTAHSSLRRHFGAALRRDLPSIEVCQLPSTSPANASWSDARQLHA
                     WRRALQSALDIPADP"
     gene            complement(12616..16344)
                     /locus_tag="VNZ06_10455"
     CDS             complement(12616..16344)
                     /locus_tag="VNZ06_10455"
                     /inference="COORDINATES: protein motif:HMM:NF017257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase subunit BcsC-related outer
                     membrane protein"
                     /protein_id="HWW21216.1"
                     /translation="MRGAVKSVWRLIGAVVAIGVAGTGLHAGDFGWPPADLALIHSAR
                     LWEIRDRGDLAQLALRKLIAARPDSPQAMVELGELDIRIGQLDGAAQVLTQLQQRFKD
                     SLARRDFEIEYRLATRDRLQLALVRHMIDMRKDKQALESLHALVPNPVPGNAMGAEYY
                     RILARIAGGWFAAFQGLRRLAAEHPDDPNYQLALARQMLSRPEYAVSGVMTLYRLAQR
                     DDIRGGDVDELLAQAFRNLPARRIPPQVLRDYLKRHADDPLVLGLLAQQRRAIEQQQL
                     LANDGLSAVEPALQQRLRQQLTQQLESPGADSVAQRLLLMLRMFGPPVQPEAVPLDRH
                     FDDSALEAAVWWERAQRALSSQHLEIAAAEMQAALAFHTRDYEATIASAEQLESLGAS
                     AESGALLTGACQLDPRSNWLFETRLRWMIAHRQFDGAIALLQRRPIAGKWSADSRDAL
                     LALALDQRASGHYAAGEIEAAIADWQAALRLTPRDPWMRYRLATIDHSGHQDDQGVAL
                     MSEGVRQAPDEPQMRYAQALYLSSLEHYEAALVSLDAIPSAARTDAMNALHERLRVAQ
                     ARQRARDLFLAGDRDGARAALLAVEPLAARDLDRARELAYAWLEIGDPQHGIGLIEPY
                     AQGTGADSSAVLLSWAQILNSADDSTRLGAVLDTLHARGLSEPDRHELARLQRALDLR
                     IVRELERDAQYQEAARRLDAMLAADPDDRALRVARAETDMSLGKPGLARDRLASLVTE
                     RPDDFDARLSYVRALTESGDLALARVQLASIQERIPADDFELQLSLARRQLGLGQANE
                     ALITLRPLLAKTPARADVLLLAARAASSQHHFALAREYFKQAADIAGAADALDARRAA
                     DEIQARMRSGLATALQVLHQPGVSGISQLDVVTVPSAWVFARGYEQRLIGHADLVSID
                     TGHLGGDYEHAALLGKVQAAGPTAALAYSNERQTGIALSGGYQTDTLAADVGTTPLGF
                     DLPNIVGGVEWTPSWRSLDLTLGLARRAVTSSALSYAGLRDPVSGQKWGGVVANGPYA
                     GIGLYRERFSLSGSIRFSELTGTHVPDNQFLGARASGSWKFYNHGDEASAYTGLVLND
                     WNYQHNLANYTFGSAGYYSPRSYLSTALPLELIGVKAGWSYQIRASIAYSVSSIAQES
                     FYPDDAALQHAAARSPLPAGFDSPLFASSHGGAFSVSAYAAVERQVTRGLVLGAMLDI
                     DRTDFYHPTVISLYLRHAFTAFDTPLAIPPRPTRPYNP"
     gene            complement(16329..17444)
                     /gene="bcsZ"
                     /locus_tag="VNZ06_10460"
     CDS             complement(16329..17444)
                     /gene="bcsZ"
                     /locus_tag="VNZ06_10460"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF008305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HWW21217.1"
                     /translation="MLACVWLCGTGLTYAAEAQCDTWPQWQRFKQLYLSADGRVIDAS
                     NPAKITVSEGQSYALTFALIANDPQSFATVLQWTQNNLSGGDLGRTLPAWQWGQTPNG
                     AWGVLDQNSASDADLWIAYTLLQAGVLWHNEHYLTLGQALARRILLDEVALIPGLGPA
                     LLPGPRGFVEHENWRLNPSYAPIQLLRAIGHQTHEKLWDQVLQSSQRLIIASAPHGAA
                     SDWLDYRIADGFVTDSVTQGIGSYDAIRVYLWAGMLPGSDPAYQAFSHQFSPLLALLA
                     ARPGLPEVIEPDSLRTRGDAHHGFYAALLPLLSRAHAQQLLQRYRARVDSDALRDDQH
                     YYNDVLSLFGTGFLEDRYHFERDGALTPQWSKPCAAQ"
     gene            complement(17485..19761)
                     /gene="bcsB"
                     /locus_tag="VNZ06_10465"
     CDS             complement(17485..19761)
                     /gene="bcsB"
                     /locus_tag="VNZ06_10465"
                     /inference="COORDINATES: protein motif:HMM:NF008323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HWW21218.1"
                     /translation="MTRALARISGVVAVMLMTLSSLRGIAATAVHTPAPLPSPPTGAT
                     SAPVREIKLTFADLGSPAPIVLHGVQSVGSVNVGTRKDEVVVGATLHLRMTYSPAMLP
                     ELSHLRVMLNGQALAAMALPKQDAGREIEREVELDPRYFSDYNQVRFDLIGHYTLECE
                     DPQHSSLWATISPQSDLTLTLRPVELRDDLALLPAPFFDLHDSRRLTLPIVLPPHASR
                     DMVQAAGIAASWFGMLADYRGARFPVHFGDLPEQHALVFATNDTRPAELTLPQVQLPT
                     ISVIDHPHNPLLKLLVFQGKDTLQLHQAVEGIVLGNDVLTGARATISRVEHKPRAAYD
                     APRWLTTDHAVRLGELVDSPEQLQATGIAPGPIRVSLHLPPDLFTWNRAGVPVDLHYR
                     YTPPIVRDNSLLAVTINDQLLRSYRLPPESESGNGDKFLVPLLQSNASRQSQGLLIPA
                     FQLASNNQMQFQFSMEFHREGLCKEIFVDNTHESIDPDSTVDISSFPHYTAMPNLALF
                     ANAGYPFTRYADLSETAIVLPDAGDRVSIEQLFFVLGRMARQTGAVALNYQLLDSVQA
                     RQAGDRDLLLLSGTPSNALLQSWGKDLSLIISAADRNYHKLPNAAASTLDRTRSSADN
                     RTQSGDVTVQSEGSLGAYMSFQSPLSSGRTVVALVGTDRAAAESLVNVLEDEGKVSGI
                     RGELSIVRAGTVQSYQGNHFYYVGSLSWWQWLWFHVSGHPVLLTLVSLIAAIAVALWI
                     YGWLQRLVAKRLETRPAK"
     gene            complement(19758..20249)
                     /locus_tag="VNZ06_10470"
     CDS             complement(19758..20249)
                     /locus_tag="VNZ06_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21219.1"
                     /translation="MAKAGSTWLPALCAGVLCALWISAQPALADGSSGKTLPHSEAEV
                     RFQQGLKLAAANQSDAAVKVFSQLTLDYPLLPQPYVQLAALYARQGKLPRAVDALRVA
                     LEHRLDDGTLQEALGDLYLELAKQSYRGAIDAANPGPNAPDKYAALQKLNSPAVIRAT
                     ATP"
     gene            20353..22893
                     /gene="bcsA"
                     /locus_tag="VNZ06_10475"
     CDS             20353..22893
                     /gene="bcsA"
                     /locus_tag="VNZ06_10475"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF008558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HWW21220.1"
                     /translation="MLHADDTVQVRPIPILTPPGRPLEVGWAQEFARWLWHLFVLPQD
                     LARPWPAWAHRCIKPSLLALATHLGVSNPRSARSWALHLILLNPPRTRWWSNPTEYQR
                     AQRRKAPLFRALLQHLAAPLLGPFIYGIAARIEVYSRIMSRAFARAHVLVRVGIVVAV
                     LLMLVLSATTPLSMGQQCILLALMWSLTMLVRQLPGYGPGIIMMVFSVVASSRYIWWR
                     LTQTTDLNPGFEWFLGVGLLVAECYTWIIMVLGYFQNARPLRRRPAPLPDDRALWPTV
                     DIYIPTYNESLQVVTPTVLAALSLDWPADKLNVYILDDGKRAAFREFAQQCGAKYIIR
                     SNNSHAKAGNLNHALGITSGEFVAIFDCDHIPVRTFLTTTMGWFDRDPNCAMLQTPHH
                     FFSPDPFERNLGTFRRVPNEGNLFYGLIQDGNDLWNAAFFCGSCAVLRRRPLLEVGGI
                     AVETVTEDAHTALKLHRRGYSTAYIRQVLAGGLATESLSSHIGQRIRWARGMAQIFRL
                     DNPMTGPGLNFFQRLCYSNAMLHFFSGVPRLVFLTAPLAYLYFQFHIINAAALTLAAY
                     AVPHLLQSSLANSHLQGRRRHSFWNEAYEAVLSWYIALPTTVALINPRLGKFNVTAKG
                     GLVEHEFFDWRISAPYLVLVTLNFVGALLAIPRMLFWNSFEADTVIINLVWTLFNLIL
                     LGAVLGVAAETRQVRSVPRVTKSQPATLYLPDGRALSCESEDFSMHGLGLRTDIPLKL
                     NRGDPLRVGLTADRIEYLFPVEVAVVRGNRIGMQLGNLSMKQQQDYVRCTFGSPDAWS
                     DWNRNTEADHPLASFAEVFSFGATGYVRLLESVYHSIVGWWRGAPRAA"
     gene            22897..>23817
                     /gene="bcsG"
                     /locus_tag="VNZ06_10480"
     CDS             22897..>23817
                     /gene="bcsG"
                     /locus_tag="VNZ06_10480"
                     /inference="COORDINATES: protein motif:HMM:NF023088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HWW21221.1"
                     /translation="MGLWSAYFFAKLLLFAGGYLDFNPWLNLAFAAFTALPPKNGRQR
                     FAKNLIAVPLSIVLLYHDSWLPPISRALSQAGNLQAFSGAYLLELLGRFISLKVLAQL
                     AAMMAVYLLARRKLRLSTFVFLAILLIGLFPQGWPSFGPAAVANAAASTTPGLTPAVE
                     VDPRNMRPEALDALLTQFYAKERQRQVRFTPAADDVAPYDILILNVCSLAWDDLNVVK
                     RDKDPLFARFDLVLSSFSSGASYSGPAAIRLLRGNCGQTEHAQLYNPPKPECLVLDGL
                     QKAGFEPHWLMNHDGQFGNFYADVRDRGGVS"
BASE COUNT         4321 a         7446 c         7622 g         4428 t
ORIGIN      
        1 ggagcacaag tcgccgcaga tgctggcact gaattttcgc ggccggctgc cggtgctgcg
       61 cgacaacgac tatgtggtat tcgagtcgct cggcattctt tactacctcg atcgcaaata
      121 cccggagccg ccgatcttcg ggcgcacgcc tgaggaatcg ggcgtcatca tgcgggtgat
      181 ctgcgagtat cagtcttatg ccgaaccgcc gctgaagcag atcacgacag cgctgcagca
      241 acgccacgat cgcaaggtgg acgagacgat cgccgaagcc atgcaccgtg tggcgggcga
      301 ggcgcgtacc atcgaacagc gcctggcgcg ctcagactgg gtcgttggtg agagcttcag
      361 tgcggccgac atgatgattt atcccgacat ccggctgctg gtgcgcatgc tgcagaagcc
      421 gcaggctcac gagctggcag cacgatttct accgatggaa gtgaactatc cggcgatcgg
      481 gcgctggctc gatcgagtcg cagcgctgcc cggccacgaa cgcacctggc cgccgcattg
      541 gcgctgatcc ccgcgaggca ctgtcgtcaa ccgtttcgcg cacgccgcgg ctgttcatcc
      601 tcccctggaa tcaagcgcta gcgacttctc ttaccgattg gtaacctgcc ggcctcttcc
      661 acgcgggtag gtcatgagcc aggacaaagt gttggtggat tttccaggac ggatcgtcgt
      721 cgtcggattc ggcagcattg gacagggagt attaccgctc atcttgcgac acatcggcgt
      781 caccgccgag cgcataacga tcgtcactgc cgaagacgtg ggtgccgacg aggcggcccg
      841 ctatggcgtc aagttcatca agcagcgcct gacacgcgag aattttcgtc gcacgctcga
      901 tccgatactc ggacgcggcg attttctgct gaacgtttcc gtcgatgtgt ccagcatcgc
      961 actgattcgc tacagctggg ataagggcgc gatgtatctg gatacctgta tcgagccttg
     1021 gcccggcggc tataccgatc ccaccatccc cgccgcgcgc cgtaccaact acgcgctgcg
     1081 cgaggaagcc ctcgcgcttc gaagcggcag cgctcgcgcg cccaccgccg tgctgaccca
     1141 tggcgccaac ccgggtctgg tatcacattt agttaaacag gcgctcctca atgttgccgc
     1201 cgatgtcggc atcggcaatg tctcgccgac ctcgcgtgcg gaatgggccg accttgccca
     1261 gcgcgtcggt ctcaaggtaa tccatgtggc cgagcgcgac acacaggtgt cgaccattcc
     1321 caaggagccc aacgagttcg tcaacacctg gtcggtggac gggttcgtgt ccgagggctc
     1381 gcagccggcg gaactgggtt ggggttcgca cgaacgcaac ttcccgcgcg atggccggcg
     1441 cttcgatttc ggcggcggct cggcgatcta tctgggacaa ccgggtgtca ccacccgtgt
     1501 gcgcagctgg acaccgaact acgggtccta ccatggcttt ctgatcacgc acggtgaggc
     1561 gatttcgatc gccgactatt tgaccgtgcg caaggacgcg caggtgttgt atcggccgac
     1621 ggtgcactac gcgtaccacc cttccgatgc cgccgtgcta tcggtgcacg agttctgcgg
     1681 tagcaactac cagatccaga atcgcaagcg cattctgatg gacgacatca gcaagggcac
     1741 tgacgagctg ggagtgctgc tggccgggca caagaagaac gcctactggt acggctcgca
     1801 gctgacggtg gatcaggctc gcgcgctggc gccctataac aatgccacca gtctgcaggt
     1861 caccgcggcc gtgctggccg gaatggtctg ggccatggaa aacccgaaca acggcctgac
     1921 cgagccggag gacatggact acaggcgcgt gctggcgctg tgcatgccct atctggggaa
     1981 gattgtcggc gagtactcgg actggacccc tttgaccgac cgtgagcgat tgtttcccga
     2041 ggatctggat aagagcgatc cctggcagtt caagaatgtg cgtgtcagct agcgctggca
     2101 gcaccccttc aggccgggta gtcgatcgcg acgatcacat agcgctgcga gccggacggt
     2161 agttccacgc tcacttcgtc gtccaatcgc ttcccgagca atgccttgcc gagcggcgca
     2221 tccatgctga tgcagtcgcc ggccagatcg atctcgtcgg gtccgacgat ccggtagcga
     2281 agctcttctc cgacgcttgt ctcgagcgtg acccgtgctc cgaagaacac gcgctggcgg
     2341 tcgcttggcg ggtggtcaac gatcaccatg ccgtccagac gcgcgcgcag aaagcgcacc
     2401 cggcggtcaa tctcgcgcag gcgacgtttc ccgtacgtgt attcggcatt ctccgacctg
     2461 tccccctgtg ccgccgccgc ctggaccgag gcggtcacct gcgggcgctc ccggcgccac
     2521 agctcctcga gttcggctct cagacgcgcg gcgcccgcgg gcgtgatgta cttggaaccg
     2581 ggtttggatg gagcacgaaa tcgacccata ttcaatactg tgcagcagct cacagccagg
     2641 gcaagggggt gggcatagcc tacgctcatc ccctgggcgg gctcacaaag cccgcttgac
     2701 tcgccctggc cttgcagccg gggcgttttt ttttagcgcc agaacgtgtg aagatccacc
     2761 cctgtcgccc agggagaaca ccaatgcgca cgttagggat gtgcctcgtc ttggccgtcg
     2821 ccatgctgtc gagcggctgc ggctacaacc ggatccagca gcaggatgaa gcagtcaagg
     2881 ccacctgggc ggaagtcgtc aatcagtacc agcgacgggc cgatctggtg ccgaatctgg
     2941 tgaacacggt caaaggcttt gcccagcagg agcagaaggt tctgatcggt gtgaccgagg
     3001 cccgttccaa ggtcggcagc atccaggcga cgccggaact ggtcaatgat ccgcaagcct
     3061 tccagaagtt cgaggcagcc cagggacagc tcacgcaagc gttgaagagt ctgatcgcgg
     3121 tcagcgaaaa ctatccgcag ctcaagtcgg accagaattt ccgcgacctg cagtcgcagc
     3181 tcgagggtac cgagaaccgg attactgtgg cgcgcaatcg ctatatccag gcggtgcagg
     3241 actacaacat caatatccgc tcgttcccga ccaatctgac ggcgatgatg ttcaagtatc
     3301 aggtcaagca gaacttcacg gtgcagaacg aagcggagat ctccaaggct cctaccgtgg
     3361 atttctcaca ctagccgcgc ccgcacgcca tgcgcctggc gctcgctgcc ctcctgctgc
     3421 tgctcgctgc agcgctgcca ggtgcgcgcg ctcaggccct gcagccagca ccgaaactcg
     3481 aaacgcgggt cacggatctg accggaacgc tgaccgccgg tcagcaggcg gaacttgagc
     3541 agaagctgcg cgatttcgag tcgcgcaaag gcgcacagat cgccgtcctg ctggtgccga
     3601 ccacgcatcc ggaagagatc gagcagtact cgattcgcgt ggtcgaggcc tggaggctcg
     3661 ggcgcaagaa agtggacgac ggcgcgttgc tcatcatcgc caagaatgac cactcgctgc
     3721 gaatcgagaa cggctacggc ctggaaggag tactgaccga tgcgatgagc aatcgcatca
     3781 tcgaagacac gatgctgcca ttgttgcggc aggatcagta ctacgcggcg atcagcgccg
     3841 gtaccgatca gatgatcaag ctgatcgatg gggaagcact gccgccgccc gatcagacct
     3901 ggcggcgccc gcgtccgctg cccctgaatc tggtggtggg cctgtttttc acgttcttca
     3961 tcgtctcaac agtgctgcgc tcgatcttcg gacgcatggc cggatcgctg ctgtgtgctg
     4021 ggggtatggg actgctggtg ttcggtcaga ctcaggtcgt ggtcttcgcg atcggcgccg
     4081 ccgtgctggc cttcgtcctg gccatgatcg gagtcggatc gggctggagc agcggacgct
     4141 atggggggta tgggggattc ggcggcggag gctttggcac aggaggatcg ggcggaggag
     4201 gtttcagcgg cggcggtggc agtttcggag gcggcggagc ctcgggccgc tggtgatgca
     4261 cttgttgctg cgcctgttac gtcatttgac caccttcgcg ctgcgtacgc ggatgctgtt
     4321 tgcgccaccg gtgctcgggc agatcgagca cgctgtcgcc gatgctgaac agcatcattc
     4381 gggagagatc cgtttcgcca tcgaaaccgc actgccgttg ccggcggtgt ggcagggcgt
     4441 gacgccgcgc gagcgtgcct tgcaggtgtt cgcgcagctg cacgtctggg acacgcacgc
     4501 caacaatggc gtgctcattt atgtcttgag ggcggatcgt gcggttgaaa tcattgccga
     4561 ccggggcatc tgcgcccgcg tcagcgaggc cgaatggcag cagctgtgtc gggcggttga
     4621 aagcgattac cgggctcagc gctatgcgca aggctcggtg gcggcggttg cgggcgtcgc
     4681 gagcctgctc ggccgacatt tccccggcgg gggctcggcc gtgaacgaac tgcccaatca
     4741 gccagtactc ctctaaaatc ggaccgatga atcggggaat gcctactaca ccatgggcgg
     4801 tcgctgtgct gaccgctacg acgctcggta cgctggcggg ctgctcgctg ttcaatggcc
     4861 gcgaagtgca tgcccccgct ccccggatgc aggctccccc gccgccgccg cccaagccgc
     4921 aattcgaagc cccgatcgat acccatactt ttacgctgac gaaccccgac gatgaggtcg
     4981 tcggcgcggt acagattacg cacgcctcga aggaagacac gctgccggac atcgcccggc
     5041 gcttcaacat cggctatgag gagatcgtgc gcgccaatcc tggcgtggat ccctggattc
     5101 cgggtgacgg gcgcagcatc gtggtgccaa cgcgatttgt acttcccgat gcaccccgtg
     5161 agggcattgt catcaatgtg gcggcgatgc gcctgtactt ctacccgccg cacaagaagg
     5221 gcgagcctca ggtcgtctat acctatccga tcgggatcgg caaagtgggc tggtccacgc
     5281 cgatgggcgt caccaagatc gtgctgcatg agaaggatcc gatctggcgc ccctcggccg
     5341 ccctgcgtaa ggaccatttc aatgacaacg gcgaggatct gcccgcggtg gtcgggcccg
     5401 gtccggacaa cccgcttggc aaatatgaaa tgagactcgg ctggcccagt tatttgatcc
     5461 acggcaccaa caaaccctac ggcattgggt tgcgttcgag tcacgggtgc atgcgcctgt
     5521 atcccgagga tatcgagaag ctctacttca tggtgccaga cggtatgtca gttcgggtgg
     5581 tcaaccaacc attcttattt ggctggaagg accggcagct ctacctgcag gcctataccg
     5641 tcctggaaga cgactcacgc gactggaacc atgcacagca gaagctgctg gcgcatgggc
     5701 tcgctcagca cacccagaag caactgaaag actccggcat tcagatcgac tgggacagcg
     5761 tcaaagcgat cacgcatgaa ccgcgtggca tcccggtgtc ggtgacgggt actgacaaca
     5821 acatcgatgc cgtgctggcg gcagcaccca cggtcgagaa tcggatcccc gagggcgccg
     5881 actgggatgg cggcgatgcc agccaggaca atggcaacag cgccaagccg ctgctgcagg
     5941 aacgtgagcc cgccgccaat gcgaagggtc cggtaacggt cccgaccgct aaacgcagtt
     6001 cgggaggctg atcgtcgtgg cgcagttgag cgaatggctg cagctcatgc tggcggagat
     6061 cgcgcgtaag caggacgagg cgcagcgcgc gagcgaagag caacagcaac gccgcaacga
     6121 ggcaccgcag ccgacagcgc cgtcgccgcc caaatatcta tcaacgcaat cctgaaacgc
     6181 tgcgtgcaac aggcgcgctc gtgcgcgctg tgttacattg actgccaggc ttcgagggta
     6241 gggcgtggcc gacctgctgg tttctgccgc tgacactgta agactaattc ggccgcaggc
     6301 cacgatccga cgtgccccga cggccattac cgcttttgtc gggcgagccc tcaaaggtcc
     6361 ggtcaaccac gcgatttcga ttgggagctt caacgattat cagcgcgtgt tcggcggtct
     6421 atggcaaccg tcgacgctgt cttacgcggt agagcaatac tttgaaaacg gcggtcgcgc
     6481 ggcgattgtg gtacgagtcg tcaacggcgg tcgtgcaccg accatgcgtc tgccggcggg
     6541 caacggctct ctcaacctga tcggcctgtc accgggcaca cgcgagttcc tgcgggcctc
     6601 tgtcgactac gacggcattc agccgcacga aaccgatcga ttcaatctga tcctgcagcg
     6661 tatccgggca ccgggatccg agttcatcga agatcaggag atcttccgcc ggctgtcgat
     6721 cctgccgggc gccgatcgca gcatcctgga cttgctcacc gattcacgcc tggcgcgact
     6781 gcatgggcca ttgccctcgc agcgacccga ccggccacgc ggcttgccgc cgattgcggc
     6841 ggtcggctat gtgatgtcga acggtgacgg tgatgacggt gatgcgctca gcaactacga
     6901 catcatcggc gatgccaaga gcggcagtgg cctgttcgcg cttcagggca ccgagcactt
     6961 caattttctg tgtgtaccgc cgttgacacg cgaactcgac gtcggattac ccgcgttgct
     7021 ggttgcacta cggatgtgtc ggctacatca cgccatgctg ctcgtggatc cgccggcacg
     7081 ctggtcagat gcctccgagg tgctgaacga cctgcgcgcc tggccgttct acagcgagga
     7141 cgcggcgatg ttctatccgc gggtacaggc ctacgatcgg ttacgggggc gacaggagat
     7201 cttcgggtca gcggccgctg ccgccggact gctcgcgcgg ctggaccgca catcaccgat
     7261 ctggtcaccg accgacaccg aggaggccca gatgcgcccg gcgctgcgcc cggccaccat
     7321 ggtcgcggac agcgaccgtg atcgactgct gcacgccggt gtcaatctgc tgcagccatc
     7381 gcgtatgccg cgtacccgaa tcaagctgcg taccctgatc cccgaagcgg gcgtcaagaa
     7441 cgaatggcgc tatctgtgcg cgcgtcgttt cgccctgttc gtgatgagca gcattgagcg
     7501 ggggacgcgc tgggtgctgt tcgaacagcc tggggcggcg ctgtgggagc gggtgcgtct
     7561 ggaagtcacc gcattcctgg aatcactcga agacgaagga gccttcgccg gtcgtagtgc
     7621 cgaggagaac tactttgtgg tctgcgacga gcgcgtcaac gacaccgagc atgtggcggc
     7681 cgggagattt cagctggtgt tcggattcgc cgattcgcgc cccggcgatt ttcagacgtt
     7741 cctggtcagc caccagccgg gacacagcag tgcgcgcccg gccagtgtca atcgttacgc
     7801 gctgctaccg caaagctgac gacggcgcgc gcgacctcct tgttgtcgcg gtcgctgacg
     7861 gtgaccggta caccgaccag gcccggatga ccccagtcga tcttgtccag tcgggccagc
     7921 gcaacggcgg cgtcacccgg ttgcgccaga tgttcgatgg tcagaccacg gctgttccag
     7981 tacagaccct ccggtacgct cacctcgatg acaaagctgg cggccagctg tgccaatgcg
     8041 gcaatggcaa tcggatcaat ctcgccgtcg atcgttcgta cccgacggtg cgcattcatc
     8101 atcgctcgac acaacccgac ccgcagttcc tgcaggtgcg cccgcaaggt cctgagcatg
     8161 ggcaggtgtt cgccggcggc gcgttcgaag cgccacttgc cccagggcca gcgttgcatg
     8221 cgctggtagc gatgcattgt caggctgaca ggcgccgtct gcgtcgtcgt cagccaatcg
     8281 agttgcgggc gcacggcgcc gagttcgctg ctgatggtgg cggcctcttg atgcggtgga
     8341 tgtcgccaac gaatcaattg gcgaccgatc cactgtattc gagttgcaat cgcccctcaa
     8401 gtctcaagga gtatgattta ggataacgat ccgtgcatgc cggaacgaat gcaaggacaa
     8461 ccacaggatg gccaatcgct acgttgtatt ttctcacggt aaggacagtg gaccgtgggg
     8521 taagaaaatc acggcgctgg ccgacgttgc ccgcgcggaa ggctacgacg tcgagtcggt
     8581 ggactatcgt ggcatcgata cgccgcgggg tcgcattgac aagctgatcg aggtctgcaa
     8641 gaagctgtcc ggcgatctgg tgctggtcgg ctcaagcctc ggaggctacg tgaccgtgtc
     8701 ggcggcgtca ttactacatg cgcgcggcgc attcatcatg gcgcctgcgc tttacgtgga
     8761 gggactgccg ccgctgcgcg agcggctgct cgattgtccg acgatgatcg tacacggctg
     8821 gaacgatgag gtcataccgg tcgagcatag tgtgcagttc gcgcgcgagt accgagcggc
     8881 actgcatttg ctggatggcg atcatcagct gcattcgcaa ttgcgcttca tcaggtatct
     8941 gttcgaatac tttttgatca gcctggactt gcccgcggag ctcggcgcgt cctgatcacc
     9001 tgaaacatgc agggcttact gcgcagaatt ctcgcactgt gggtgcgagc cacgttgcgt
     9061 ccggaggacg ccgcggcgcg actcaatggg cgcacggtac cgatctgcta cgtgctggag
     9121 cgtgtcagcg ccagcgacct ggcggtgctg cagaacctct gcgtgcgcga tcgactgcca
     9181 cgcccgggac ggcggctgct ggcacgtcag ctgcgcagcg cccgctcctc gctcgctctg
     9241 acgcgcgcag tcggcttctg gcgcagtcgc gacgaccggc gaccaccgga gctgctgctc
     9301 aatctcatca acgcgttgcg cactgatctg agcctcgatg tcagtctggt gccggcggcg
     9361 gtctactggg gacgcgcacc acagcgagag cgttcgctga tccgcctgtt ggtctcggaa
     9421 gactgggtca tcgcgagccg ggtgcggcgc gtgattacgg tgatcttcaa cggccgcaat
     9481 accacggtgc agctcggtga tccgatctcg ctgcgcagcc tgctgggcac cgaggccgac
     9541 acccgggtga gcgcacggcg cgtttcgcga ctgctgcgta cgcagctctc gcgtctgcgc
     9601 acggcgctga tcggtccgga cctgtcgcac cggcgcacga tcgtggccga agtactgcgt
     9661 acccgtgcgg tgcgcgccgc ggtggcccag gaggtgcgcg aacaggacca gtcgacccgt
     9721 cgcagcgtat tgctcgaggc ccgacgctac gccaacgaga ttgcagccaa ctattcgcac
     9781 acgttcgtca gcctgatggc catggggctc gccaagctgt ggaaccggct gtacgacggt
     9841 gtcgagtttg aacacctgga taggctggag cagctcgcgc agggcaacga aatcatctac
     9901 gtgccctgtc atcgcagtca catggactac ctgctgctgt cgtatgcgat ttatcagcat
     9961 ggctacgccg tgccgcatat tgcggccgga ctgaatctga atctgccgat cgtcgggcgg
    10021 ttcctgcgca agggaggtgc attctttctg cgtcgatcgc tgcgtggcag ctcgctctac
    10081 accgttgtct tcatgaagta tctggcaacg atgatggccc gcggtcactc gatcgaatac
    10141 ttcatcgaag gagggcgcag ccgcaccggg cgcctgcggc caccgatggg cggcatgctg
    10201 gcgatgaccg tgcgcagcta tctgcgcgag ccgcgccgcc cgctggtgtt cattccggtg
    10261 tacttcggct acgagcgcat cgtcgagggc tcgacctacg tcggcgaact gtcagggcgg
    10321 ccgaaagaga aggaatcggt gctcgggctg ctgcgaatga tcggcaagct acggcagcgt
    10381 ttcggacgcg tacacgtcaa tctcggggag ccgctctatc tggatgcgct gatcgaccag
    10441 tttcgccctg gctggcgtca ggaggaattc gatgatcaga atcgcgcgcc ctgggtcagg
    10501 gcgctggtcg aagagctcgc gctgcgcatc atgcgccgga tcaacgccgc cgcgacggtg
    10561 acaccgatca acttgctggc gatggcgctg ctcgggacgc agcgccaggc catgctggag
    10621 accgatctga tctcgcagat tgaagccatg ctcgcggtat tgcgggccgt gccctacagc
    10681 aatctggtca gcctgagcac ccagagtgcg ccagagatga tcaagtacgg gctcgagctg
    10741 aacgtgatca ctcgtgagaa tcagcgtgcg ggacacatcg tgcgcatgag cccggaacac
    10801 gcggtgctcg cgacgtacta tcgaaacaac gtgctgcatc tgttcgcgat gccgtcactg
    10861 atcgcctgct gcttcctgtc gaattcgatg atgcgcaccg aggacgtgct gcggctggcc
    10921 tcgcgtatct atccgtacat tgcggcagaa ctgttcctgc ggtggagcga ggatgaagtg
    10981 cctgcagtgg tggcggaatt gctggctgcg atggcgcaac tggggttgct ggtacgcgaa
    11041 ggcgatctgt ggcatcgccc ggcaccggtt tcggctgccg ccatgcacct gtcgctgctg
    11101 gcgcagagca cgatccagac gatcgaacgc tactatctgg cgatcgcgct gctgatccgc
    11161 tcaggcagtg gcgccatcac ccagaagctg ctcgaagaga actgcgagct catggcccag
    11221 cgaatgacgg cgctgtacgg tttgaattcg cccgaattct tcgatccgag cctgtttgag
    11281 agcttcatcg atctgctgcg cagtcgcgac gtcgttcgca ccgacgaggc cggccgactg
    11341 acgtttgacg aggtgctgct gcgggtcgaa gccgacgcgc agctggtgct gtccgagcag
    11401 atccgacata gcatcctgca ggtcacgcac agctgacccg cccctcaacg cggccacagc
    11461 cacttttcta ccgctacggc aacgaagtta cgcgctaacg cggccacagc cacccgaata
    11521 ccccggcagt cagcagtgaa tacaccagtc cgtcgaacat gtagcgcact gtgatgcccc
    11581 aaccgcgctg ccaccagatc gattcatgcg cgtaggcgac cgaataggcc atgaagccga
    11641 cggtgccgac gactcgaaac acattcagat agggcgcagc gaatgccagc gtgcggctgg
    11701 cgacgtaggc gcacaggaac gatatcgcca actggtagag aaaccattgc accagcgcgc
    11761 tgccgagctg cacgccgccc ggtcgcatca cggtcatgat cacggtgact ggggcgctgc
    11821 tgcgctgtga ttgcgaggca tggggaatga aataatcccc tggggtcagt cccagcgacc
    11881 ccagggactg acatgctgac ggctcattgg ggacagcctt gaagtcgttg cggtggtgac
    11941 gcacaaacat gtgcattagc cagccggcca tgaaggcgat cactgcgctg agaagtatcg
    12001 gcagccacag agccacgagc gtcaccatgc gcctcccctc ccaggcatat tgtcaggcag
    12061 atgtactacg ggtcggcggg gatgtcgagt gcgctttgca gcgcgcgccg ccaggcgtgc
    12121 agttggcggg catcgctcca gctggcattg gcagggctgg tcgaaggcag ctggcatacc
    12181 tcgatcgacg gcaggtcccg ccgcagggct gcgccgaaat gtcgccgcag gctcgaatgc
    12241 gccgttccac cgttgcagca aatacgtcgt atcgacgggg tcgctcgcag caatcccatc
    12301 agatcattcg gcttggcgct gtgatgctcg atcgcggcat ccagactgcc gggtcgtaca
    12361 caggagtgca gcacatccca caatgccaga tgatgagcgc gcaacaattg aagccgcgca
    12421 tcgtagcgca gcggccctgt gatgccgcag agtctctcca ggatgctcca gaagcggttg
    12481 cgcgggtggg cgtagtagtg atgagcctcg agcgatgcaa cgcccggcat actgcccaga
    12541 atcaggatac gcgcgtcgtc cgcgacgatc gccggaaaac tgtagacccg cacgtatcga
    12601 gtctaacgca acgcactagg ggttgtacgg acgagtcggt cgtggtggga tcgccagcgg
    12661 cgtgtcgaag gcagtgaacg cgtggcgcag ataaagcgag atgactgtcg ggtgatagaa
    12721 gtcggtgcgg tcgatatcga gcatcgcacc cagtaccagc ccgcgcgtga cctgtcgctc
    12781 caccgcggcg taggctgaga cgctgaatgc gccgccgtgg gacgacgcga atagcggtga
    12841 gtcaaaaccg gccggcaaag gtgagcgagc cgctgcatgc tgcaacgctg cgtcgtccgg
    12901 atagaaactc tcctgcgcga tcgagctcac gctataagcg atcgaggcgc ggatctggta
    12961 gctccagccg gctttcacgc cgatcagctc cagcggcagc gcggtactca gataggaacg
    13021 cggactgtag tacccagcgc tgccaaaggt gtagttggcg aggttgtgct gatagttcca
    13081 gtcgttgagc acgaggccgg tgtaagcgct ggcctcgtcg ccatggttgt agaacttcca
    13141 gctgccggaa gcgcgcgcac cgagaaattg attgtccggt acgtgcgtac cggtcagctc
    13201 agagaatcgt atcgaaccgg agaggctgaa acgctcacga tagagcccga taccggcata
    13261 cggaccgttg gccaccacgc caccccattt ctgaccgctg accgggtctc gcagtccggc
    13321 atacgatagc gcactgctgg tgacggcccg gcgcgcgagc cccagcgtca ggtcgagcga
    13381 gcgccagctc ggcgtccact cgacaccgcc cacgatgttg ggcagatcaa accccagtgg
    13441 tgtcgttccg acatcggccg ccagcgtgtc ggtttgatag ccgcctgaca gcgcgatgcc
    13501 ggtttgccgc tcgtttgaat aggctaacgc cgcggtggga ccggctgcct gcaccttgcc
    13561 gagcagcgcc gcatgctcgt agtcgccgcc cagatgaccg gtgtcgatcg agaccagatc
    13621 ggcatggccg atcaagcgct gctcgtagcc gcgcgcgaag acccaggccg aagggacagt
    13681 gaccacatcg agttgcgaga tgcccgagac gcccggctga tgcagcacct gcagggcagt
    13741 ggcgaggccg gagcgcattc gcgcctggat ctcatcggcc gcacgccggg catcgagcgc
    13801 atcggcagca ccggcgatgt ccgcggcctg tttgaagtac tcccgggcaa gcgcgaaatg
    13861 atgctgcgat gacgcggcgc gcgccgccag cagcagcacg tcagcgcgtg ccggggtttt
    13921 tgccagcagt ggccgcaagg tgatcagcgc ctcattggct tgtccgagcc cgagctggcg
    13981 ccgagcgagg ctcagctgca gctcgaagtc atcggccggg atacgctcct gtatcgatgc
    14041 caactgcaca cgcgcgagcg ccagatcacc tgattcggtc agcgcgcgca cataggacag
    14101 gcgcgcatcg aagtcatccg gtcgctctgt gaccaatgac gccagacgat cgcgcgccag
    14161 tccgggcttg cccaggctca tatccgtttc ggcccgcgcc acgcgcaatg cgcgatcgtc
    14221 gggatcggcg gccagcatcg catccagccg cctcgcggct tcctgatact gtgcgtcgcg
    14281 ttcgagctcg cgcacgatgc gcaggtcgag tgcgcgctgc agtcttgcca gctcgtgtcg
    14341 atccggctcg ctcaagcctc gcgcgtgcag tgtgtcgagc accgctccga gccgggtgct
    14401 gtcatcggcg ctgttgagga tttgcgccca gctcagcaat accgccgacg aatcggcgcc
    14461 ggtcccttgc gcgtacggtt caatcagccc gatgccatgt tgcggatcgc cgatttcgag
    14521 ccatgcatag gccagttcgc gcgcccgatc aagatcgcgc gctgccagcg gttcgaccgc
    14581 cagcaaggca gcgcgtgccc cgtcgcgatc accggccagg aacaggtcac gagcccgctg
    14641 gcgcgcctgt gccaccctca ggcgctcatg cagcgcgttc atggcatcgg tgcgggcggc
    14701 gctggggatc gcatcgaggc tgaccagtgc cgcttcatag tgctcgagac tggacagata
    14761 cagcgcctgc gcatagcgca tctggggctc atccggagcc tgtcgcacgc cttcactcat
    14821 cagcgcgacg ccctgatcgt cctgatgccc agagtgatcg atcgtggcca gccgatagcg
    14881 catccaggga tcgcgcgggg tgagccgcag ggctgcctgc cagtccgcga tcgccgcctc
    14941 gatctcgccc gctgcgtagt gcccgctggc gcgctggtcg agtgccagcg ccagcagcgc
    15001 gtcgcgcgaa tcggcgctcc acttgccagc gatcggtcgc cgctgcaaca gtgcgatggc
    15061 gccgtcgaac tgtcggtggg cgatcatcca gcgcaggcgt gtctcgaaca gccagttcga
    15121 acgcgggtca agctggcagg cgccggtcag tagcgcaccg ctctcggccg acgcacctaa
    15181 cgattcgagc tgctcggcgc tggcgatggt cgcctcatag tcgcgagtat ggaacgccag
    15241 cgccgcctgc atctcggcgg ccgcgatctc caggtgctgc gacgagaggg cgcgctgagc
    15301 gcgctcccac cacaccgccg cttcgagcgc gctgtcatcg aagtgccggt ccagcggcac
    15361 ggcctcgggt tgcacgggcg gcccgaacat ccgcagcatc agcaacagcc gctgcgcaac
    15421 ggagtctgcg ccgggtgatt cgagctgctg cgtcagttgc tgtcgcagcc gctgctgtag
    15481 cgccggttcg actgccgaca ggccgtcgtt ggcaagcagc tgctgttgtt cgatggcgcg
    15541 gcgctgctgt gcaagcagcc cgagcaccag cggatcgtcc gcatgcctct tcagatagtc
    15601 gcgcagcacc tggggtggaa tgcgtctcgc cggcaggttg cgaaacgctt gcgccagcag
    15661 ctcatcgaca tcgccgccgc gaatgtcgtc gcgttgcgcc aggcgataga gggtcatgac
    15721 tcccgacacg gcatattcgg gccgactgag catttgccgc gcgagtgcga gctgatagtt
    15781 cggatcgtcc ggatgctcgg ctgcgaggcg gcgcagcccc tgaaacgcgg caaaccagcc
    15841 gcccgcaatg cgcgccagga tacggtagta ctccgcgccc atcgcattgc ccggcactgg
    15901 gttcgggacc agggcgtgca gcgactcgag tgcctgtttg tcctttctca tatcgatcat
    15961 atgacgcacg agcgccagct gcagccggtc acgcgtggcc aaccgatatt cgatctcgaa
    16021 atcgcgcctc gccagcgagt ccttgaagcg ctgctgcagc tgcgtcagta cctgcgcggc
    16081 gccatcgagc tggccgatgc ggatgtccag ctcgcccagt tcgaccatgg cctgcgggga
    16141 gtcggggcgg gccgcgatca gcttgcgcaa agcaagctga gccagatcgc cgcggtcgcg
    16201 gatctcccac aggcgagcgc tgtgaatcag cgcgaggtcc gcgggcggcc agccgaagtc
    16261 gccggcatga aggcccgtgc ccgcgacgcc gatcgcaact accgcgccga tcagacgcca
    16321 cacgctcttc actgcgccgc gcacggtttg ctccactgcg gcgtgagtgc accatcacgt
    16381 tcgaagtgat agcgatcttc gaggaagccg gttccgaaca ggctcaatac atcgttgtag
    16441 tagtgctgat catcgcgcag cgcatccgaa tcgacccgcg ctcgatagcg ttgcagcagc
    16501 tgctgtgcgt gcgcacggga cagcagcggc agcagcgctg cgtagaagcc gtggtgcgca
    16561 tcgccgcgcg tgcgcaagct gtcaggctcg atgacctcgg gaagccccgg gcgcgctgcc
    16621 agcaacgcaa gcagaggtga gaactgatgg ctaaaggcct ggtacgccgg atcggagccc
    16681 ggcagcatgc ccgcccacag atacacccgg atggcatcgt agctgccaat accctgcgtg
    16741 accgaatcgg tgacgaagcc gtcagcgatt cggtagtcca gccagtcgga agccgccccg
    16801 tgcggggcgg aggcgatgat cagacgctgc gaggactgca gtacctgatc ccacagtttc
    16861 tcgtgggtct gatggccgat cgcgcgcagt agttgaatgg gggcatagct gggattgagc
    16921 cgccagttct cgtgttcgac gaagcccctg ggaccgggca gcagcgccgg accgagtccg
    16981 ggaatcaacg ccacttcgtc gagcagaatg cgcctggcca gcgcctgacc caaggtcaga
    17041 tagtgctcgt tgtgccacag gacgccggcc tgcagcaggg tgtacgcgat ccacaggtcc
    17101 gcatcggacg ctgagttctg atccagcacg ccccaggcgc cgttgggtgt ctgcccccac
    17161 tgccaggcgg gcagcgtccg gccgagatca ccgccggaca gattgttctg cgtccactgc
    17221 agtactgtgg caaaggactg cgggtcattc gcgatcaagg cgaaagtcag tgcataggac
    17281 tgtccttcgg ataccgtgat tttggccgga ttgcttgcgt cgatcacccg tccgtccgca
    17341 ctgaggtaca gctgcttgaa gcgctgccac tgtggccagg tatcgcactg cgcttctgcg
    17401 gcgtaggtga ggcccgtgcc gcatagccag acgcaggcca gcacggcccg acacgcggcc
    17461 gccagcctgc gcgcagcgcg cggctcattt ggctgggcgc gtttccaggc gtttggcgac
    17521 gagccgctgc agccagccat agatccacag tgccacggcg atcgcggcga tcagagagac
    17581 cagcgtcagc agtaccgggt ggcccgagac gtggaaccac aaccactgcc accaggagag
    17641 tgaaccgaca tagtagaagt gattgccctg gtaactctgt acggtgcctg cgcgcacgat
    17701 cgacaactcg ccgcgtatgc cactgacctt gccttcgtct tccagaacat tcaccagcga
    17761 ttcggcggcc gcgcgatcgg tacctaccaa tgccaccacg gttcggccgg agctcagtgg
    17821 tgactgaaag ctcatatagg cgccgagcga gccttcggat tggaccgtga cgtctcccga
    17881 ctgagttcgg ttgtccgccg atgagcgcgt ccgatcgagt gtcgaggccg cagcgtttgg
    17941 gagtttgtga tagttgcgat ccgcggcgct gatgatcagc gacaggtcct taccccagga
    18001 ctgcagcagc gcattggatg gcgtgccgct cagcagcaac agatcgcgat cgcccgcctg
    18061 gcgcgcctgc accgagtcca gcagctgata gttgagcgcc accgcgccgg tctgccgagc
    18121 catccggccc agcacgaaga acaactgttc gatgctgacg cgatcgcccg cgtccggcag
    18181 cacgatcgcg gtttcggaca gatccgcgta gcgagtgaac ggatatcccg cattcgcgaa
    18241 cagcgccaga ttgggcatcg cggtgtagtg cggaaagctg gagatatcga cggtcgaatc
    18301 cggatcgatc gattcgtggg tgttgtctac gaagatctcc ttgcacaggc cttcgcgatg
    18361 gaattccatc gaaaactgaa actgcatctg gttgttgctg gcgagctgga aggccggtat
    18421 cagcagtccc tggctctgac gcgaggcgtt gctctgtagc aggggcacga gaaacttgtc
    18481 gccgttaccg ctctcggatt caggaggcag ccgataggag cgcagtaact ggtcgttgat
    18541 cgtcaccgcc agcagcgagt tgtcgcgcac gatcggtggt gtgtagcgat agtgcagatc
    18601 caccggaaca ccggcgcgat tccaggtgaa cagatccgga ggtaaatgca ggctaacgcg
    18661 aattggccca ggcgcgatgc ccgtggcctg caactgctcg ggtgagtcaa ccagctcgcc
    18721 gagcctcacg gcatgatcgg tagtgagcca gcgcggagca tcgtacgctg cgcgcggctt
    18781 gtgctcgacg cgtgagatgg ttgcgcgagc accggtcagt acgtcgttac cgagcacgat
    18841 gccctcgacc gcctgatgca gctgcagcgt gtccttcccc tggaacacca gcaatttgag
    18901 cagcggattg tgcgggtgat cgatcacgct gatggtgggt aattgcacct gcggcagcgt
    18961 aagctcggcc ggccgtgtgt cattggtcgc aaacaccagc gcatgttgtt ccggcagatc
    19021 gccgaagtgc accggaaagc gggcaccgcg atagtcggcc agcatgccga accaggaggc
    19081 ggcaattccg gccgcttgca ccatgtcacg cgaggcatgt ggcggcagca cgatcggcag
    19141 ggtcaggcgc cggctatcgt gcaggtcgaa gaacggtgcc ggcagcagcg ccaggtcatc
    19201 gcgcagctcc accggtcgca gcgtgagcgt cagatcgctc tgcgggctga tcgtggccca
    19261 cagcgaagag tgctgcggat cctcgcattc cagcgtgtag tgcccgatca gatcgaagcg
    19321 cacctggttg tagtcgctga aatagcgtgg gtcgagttcc acctcacgct cgatttcacg
    19381 accagcgtcc tgcttgggca gcgccatggc ggcgagtgcc tgaccattga gcatcactcg
    19441 caggtgcgag agctccggca gcatggccgg cgagtaagtc atccgcagat gcaacgtcgc
    19501 gccgacgacc acttcgtcct tgcgtgtgcc gacgttcaca cttccgaccg actgcacccc
    19561 gtgcagcacg atcggagcgg gtgatcccag gtccgcaaat gtgagtttga tctcacgtac
    19621 cggggcgctg gttgcccccg tcggcggcga tggcagtggc gctggggtgt gaacggccgt
    19681 ggcggcaatc cctcgcaggc tggagagcgt catcagcatc accgccacga cgcccgaaat
    19741 gcgggccagt gccctggtca tggagtcgcc gttgcgcgga tcacggccgg cgaattgagc
    19801 ttttgcaggg ctgcgtactt atcaggggca ttggggccag gattggccgc atcgatggcg
    19861 ccgcggtaag actgttttgc cagctcgagg tacagatcgc ccagagcttc ctgcagtgtt
    19921 ccgtcgtcca gcctgtgctc gagcgcgaca cgcagcgcat ccaccgcccg tggcaacttg
    19981 ccctgtcgcg catagagcgc ggcaagctgc acatagggct gcggcaatag tgggtagtcc
    20041 agcgtcagct gcgagaagac tttgacggcg gcatcacttt ggttggcggc ggcgagcttg
    20101 agtccctgct gaaaacgcac ttcggcctcc gagtgcggca atgtcttgcc gctcgagccg
    20161 tcggccaggg ccggttgagc actgatccac agtgcacaga gcacgccggc gcatagcgcc
    20221 ggaagccacg ttgaacctgc ttttgccatg acgttaggtt cgcgtggtca agcacttatc
    20281 gtccaatagt cgttgctgga agtgataagc ggaccacatt tgcgcaggcc cggcaccgct
    20341 atcttgaacc gcatgctcca tgcggacgac accgttcaag ttcgacccat cccgatcctc
    20401 actcctccgg ggcgtccgct ggaagtgggt tgggcacagg agttcgcgcg ttggctgtgg
    20461 catctgttcg tgctaccgca ggatctggcc cggccgtggc cggcgtgggc tcatcgctgc
    20521 atcaagccgt cattgctggc cctcgcgaca catctggggg tcagcaatcc gcgcagcgca
    20581 cgctcctggg cgctgcactt gatacttctg aacccgcccc gtacccgctg gtggtccaat
    20641 cccactgaat atcagcgtgc gcagcgtcgc aaggctccat tgttccgggc gctgctgcag
    20701 catctggccg cgccgcttct cgggccgttc atctacggca tcgcggcgcg catcgaggtt
    20761 tattcccgga tcatgagccg ggcgttcgcg cgtgcgcacg tgctggtacg agtgggcatc
    20821 gttgtcgcgg tcctgctgat gctggtgctg tcagcgacca caccactgag catgggacag
    20881 caatgcatcc tgctggcatt gatgtggtcc ctgacgatgc tggtgcgcca gcttcccggc
    20941 tacggaccgg gcatcatcat gatggtgttc tcggtggtcg cctcctcgcg ctacatctgg
    21001 tggcgcctga cgcagacgac cgacctgaat cccggatttg aatggttctt aggcgtaggg
    21061 ctgctggtgg ccgagtgcta cacctggatc atcatggtgc tcggctactt ccagaacgcg
    21121 cggccgctgc ggcgccgtcc agcgccattg ccggatgatc gcgcgctgtg gccaaccgtg
    21181 gatatctaca tcccgaccta caacgagtcg ctgcaggtcg tcacaccgac ggtgctcgcc
    21241 gcactcagcc tcgactggcc ggccgacaaa ctgaacgtct acatcctgga cgacggcaag
    21301 cgagcggcgt ttcgggagtt cgcacagcag tgcggcgcga aatacatcat ccgcagcaac
    21361 aactcacatg cgaaggcggg caatctcaac cacgcgcttg gcatcacgag cggtgagttc
    21421 gtcgcgatct ttgactgtga tcacatcccg gtcaggacct tcctcacgac cacgatgggc
    21481 tggttcgacc gcgaccccaa ctgcgcgatg ctgcagacgc cgcatcactt cttctcgccc
    21541 gatccgttcg aacgcaacct cggtaccttc cggcgcgttc ccaacgaagg caacctgttc
    21601 tacggactga tccaggacgg caatgacttg tggaacgcgg cgttcttctg cggctcgtgt
    21661 gcggtgctgc gccggcgtcc gctgctcgaa gtaggtggca ttgcagtcga gaccgtcacc
    21721 gaggacgccc acaccgcgct caagttgcac cgacggggtt attcgaccgc ctatatccgg
    21781 caggtgctgg ccggcggcct ggccaccgag agtctgtcga gccacattgg ccagcgtatc
    21841 cgctgggcgc gcggcatggc gcagatcttc cggctcgaca atccgatgac ggggcctggt
    21901 ttgaacttct tccagcgcct gtgctactcg aacgcgatgc tgcacttctt ctcgggcgtt
    21961 ccccggctcg tgtttctgac cgcaccgctg gcgtacctgt atttccagtt tcacatcatc
    22021 aacgcggccg cactgacgct ggcggcgtac gctgtgccgc acctgctgca gtcaagtctc
    22081 gcgaactcgc acctgcaggg acgtaggcgc cactcgttct ggaacgaggc ctacgaggcg
    22141 gtgttgtcct ggtacatcgc gcttccgacc acggtggcgc tgatcaaccc gcggctcggc
    22201 aaattcaacg tcacggccaa gggaggcctg gtcgagcatg agttcttcga ctggcgcatc
    22261 tccgcgccct acctggtatt ggtcacgctg aattttgtcg gcgccttgct ggccattccg
    22321 cggatgctgt tctggaactc gttcgaagcc gacacggtca tcatcaatct ggtgtggacg
    22381 ctgttcaatc tgatcctgct cggcgcggtg cttggagttg ccgccgaaac gcgtcaggta
    22441 cgttcagtgc cgcgggtcac caagagtcag ccggcgacgc tgtacctgcc cgatggccga
    22501 gcgctgtcgt gcgagagtga ggatttctcg atgcacggcc tggggctgcg caccgacata
    22561 ccgctgaagc tcaatcgcgg cgacccactg cgagtgggcc tgaccgccga tcgcatcgag
    22621 tacctgtttc cggtcgaggt ggcggtcgtg cgtggcaatc gtatcggcat gcagctcggt
    22681 aacctgtcga tgaagcaaca acaggactac gtacgctgca cgtttggttc gccggatgcg
    22741 tggagcgatt ggaatcgcaa taccgaggcc gatcatccgc tggcgagttt cgccgaggtg
    22801 ttctcgttcg gtgccaccgg ctatgtgcgt ctgctcgaga gtgtctatca cagcatcgtt
    22861 ggttggtggc gtggcgcgcc cagggcagcc tagcgcatgg gtctctggag cgcctatttc
    22921 tttgccaaac tgctgctgtt cgccggtggc tacctcgatt tcaatccatg gctgaacctg
    22981 gcgttcgctg ccttcacggc gctgccgccg aaaaatggaa ggcagcgctt tgccaagaat
    23041 ctgatcgcgg tgcccttgag catcgtgctg ctctatcacg actcctggct gccgccgata
    23101 tcccgggcgc tgtcgcaagc ggggaacctg caggcgtttt ccggcgcgta tctgctggag
    23161 ctgctgggaa ggttcatcag cctgaaagtg ctcgcgcagc tggccgctat gatggcggtc
    23221 tacctgctgg cacgccgcaa gctgcgtctg agcacgtttg tgttcctcgc gatcctgctg
    23281 atcggcctgt ttccacaggg ctggccgagc ttcgggccag ccgcggttgc caacgctgcc
    23341 gccagcacga cccccggtct gacgcccgca gtcgaagtcg atccgcgcaa catgcgcccc
    23401 gaggccctcg acgcgctgct gacacagttc tacgccaagg aacgacagcg tcaggtgcgc
    23461 tttacaccgg cggcggacga tgtggcgccg tatgacatcc tgatactgaa tgtctgctcg
    23521 ctggcgtggg atgatctgaa cgtcgtgaag cgtgataagg atccgctgtt cgcgcgtttc
    23581 gatctcgtgc tatcgagttt cagttccggc gcgagctaca gtggtccggc agccatccgg
    23641 ctgcttcggg gcaattgcgg tcagaccgaa cacgcgcagc tgtacaatcc gcccaaaccc
    23701 gagtgtctgg tgctcgacgg cctgcagaag gcgggttttg agccgcactg gctgatgaat
    23761 catgacggac agttcggcaa cttctatgcc gatgtgcgcg atcgtggtgg cgtctca
//
LOCUS       DATMRM010000350         3773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4559311, whole genome shotgun sequence.
ACCESSION   DATMRM010000350 DATMRM010000000
VERSION     DATMRM010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4559311"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VNZ06_10485"
     CDS             <1..838
                     /locus_tag="VNZ06_10485"
                     /inference="COORDINATES: protein motif:HMM:NF024682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21222.1"
                     /translation="AGQEALNDCMREHGGFEHNLQSLRTVDELEERYALFPGLNLTFE
                     CREGILKHCSLPNAKQLGDVGQRFLLRQQPGLEAQLANLADEIAYNNHDVDDGIRAGL
                     ITLEEAAQVPLVQRHYQQVMSQYPDLQGRRRVHETVRRIVNEVCIDVIQSSAQRLLQA
                     EPLDIDAVRAQSQPLMGMSEPMRAQHLELKQFLREQVYRHQRVLRMTTKAKRVVRELY
                     GAFMDDIKLMPAEYRDAAQRAQDQGGIAGRARIVADYIAGMTDRYAIVEYRRLFDPAE
                     RT"
     gene            complement(844..2547)
                     /locus_tag="VNZ06_10490"
     CDS             complement(844..2547)
                     /locus_tag="VNZ06_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWW21223.1"
                     /translation="MDRFWLREYPPGVPADIDPHEFASLKALIEDACQRYADRIAYRS
                     MGTALTYARMERDSRSFGAWLQLQAGLKSGDRIAIMLPNTLQYPIAMFGALRAGLTIV
                     NTNPLYTAAELEHQLNDSGAAAVVVLENFAATLQQVLPRTSVRCVIVTAIGDRLSWPM
                     GSIANFVLRHVRKQVPTFAIRSARRFNQVISQGQRQALRPVTLDHNDLAYLQYTGGTT
                     GVAKGAMLTHGNMVANILQARAWFQQLPMERTTFVCALPLYHIFSLTANCWLFAALGG
                     TGLLIANPRDFPTFIKDLRKYPPMFFMGVNTLFNALMHTPQFNGIDFSHLVVTVAGGM
                     ALQSGVAAQWKRMTGCKITQGWGLTEASPVGCANKTIGDDTDFNGSIGLPLPSTEITI
                     RDDDGRELPIGEVGEICLRGPQVMRGYWQRPEETAQVMLPDGWLRTGDIGRIDNRGYV
                     FIEDRKKDMIIVSGFKVYPNEIEDVLGKCPGVLEVAAVAQPDAHSGEVVALYIVKKDP
                     SLTEDAVRKYAHDHLTNYKRPHAIIFRDELPKTNVGKILRRQLRDELARVVAGSQGSH
                     P"
     gene            complement(2583..3233)
                     /locus_tag="VNZ06_10495"
     CDS             complement(2583..3233)
                     /locus_tag="VNZ06_10495"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HWW21224.1"
                     /translation="MGILLALWVAVAVPTALAAGSSTTPAALGHEPVILVFGDSISAG
                     YGIRVEHGWVNLLAQKITQLGYGFRVVNASVSGETTAGGLGRLPHTLEVQHPNILILE
                     LGGNDGLRSLPLETTHDNLDKMIQLARAQGVQVVLVGMRMPPNYGERYTSGFQKMYTD
                     LAQQYQLPLVPFLLEKVALTPTLMQADGIHPTEPAEPLLLDTVWPKLEPLLGSRKP"
     gene            3232..>3773
                     /locus_tag="VNZ06_10500"
     CDS             3232..>3773
                     /locus_tag="VNZ06_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003189864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWW21225.1"
                     /translation="MKRDIVLEARNLTKQVNSPEGPLTIVRDISFSVTQGESVALVGP
                     SGAGKSTVLALLAGLDLPSSGEVVLSGQTLSSLNEDGRAKLRAERVGFVFQSFHLVPS
                     LTALENVMLPLELSGRSDARERATQMLSRVGLNRRLGHYPRQLSGGEQQRVALARASV
                     GPPAVLFADEPTGNLDSVTG"
BASE COUNT          696 a         1162 c         1205 g          710 t
ORIGIN      
        1 cgccggccag gaggcactca atgactgcat gcgcgaacat ggcggtttcg agcacaacct
       61 gcagtcgctg cgcacggtcg atgagctcga agagcgctat gccctgtttc cagggctcaa
      121 tctgacgttc gagtgtcgcg agggcatcct gaaacactgc tcgctaccga acgcgaaaca
      181 gctcggcgac gtgggccagc gatttctgct gcgacagcag ccggggctag aggctcaact
      241 ggccaatctc gcggacgaaa tcgcgtacaa caatcacgat gtggacgacg ggattcgggc
      301 cggattgatc acgctcgagg aagcggcgca ggtgccattg gtgcagcggc actatcagca
      361 ggtcatgagc cagtatccgg atctgcaggg caggcgccgg gttcacgaga ccgtccgccg
      421 catcgtcaat gaggtgtgca tcgatgtcat ccagtcgagt gcacagcggc tgctgcaggc
      481 cgagccgctc gatatcgatg ccgtgcgggc acaaagccag ccgctgatgg gtatgagcga
      541 gcccatgcgc gcccagcacc tggagctgaa gcagttcctg cgtgagcagg tgtaccgcca
      601 tcagcgcgtg ctgcgtatga ccaccaaggc gaaacgcgtg gtaagagagc tctatggtgc
      661 cttcatggat gacatcaagc tgatgccggc cgaatatcgc gacgctgcgc aacgcgccca
      721 ggaccagggc ggcatcgcgg gccgagcgcg catcgtcgcc gattacatcg ctggcatgac
      781 tgaccgctat gcgatcgtcg agtatcgccg gctgttcgat ccggccgagc gcacctagga
      841 cgcttaggga tgggagccct gggatccggc gacgactctg gccagttcgt cgcgcagttg
      901 cctgcgaagg atcttgccca cattggtctt cggcagctca tcgcggaaaa tgatcgcgtg
      961 cgggcgcttg tagttggtca ggtgatcgtg cgcgtacttt cggaccgcat cctcggtcaa
     1021 tgacggatcc ttcttcacga tatacagtgc gacgacctcg ccggaatgag catccggctg
     1081 cgcaaccgcc gccacttcga gcacgcccgg gcatttccca agtacatcct cgatttcgtt
     1141 cggatacacc ttgaacccgg acacgatgat catgtccttc ttgcgatcct caatgaagac
     1201 gtagccgcgg ttgtcgatgc gcccgatgtc accggtgcgc agccagccgt cgggtaacat
     1261 cacctgcgcg gtctcctcgg gacgctgcca gtaaccgcgc atcacctgcg ggccgcgcaa
     1321 acagatctct cccacctcac caatcggcag ttcacgccca tcgtcgtcgc gaatcgtgat
     1381 ctcggtggac ggtagcggca gaccgatcga gccgttgaag tccgtgtcat ccccgatggt
     1441 cttgttggcg cagccgaccg gtgaggcctc ggtcagtccc cacccctggg tgatcttgca
     1501 gcctgtcatc cgcttccatt gcgcagcgac gccgctttgc agcgccatgc cgccggcgac
     1561 cgtgaccacc agatgcgaga agtcgatgcc gttgaattgc ggcgtgtgca tcagcgcgtt
     1621 gaacagcgtg ttcacaccca tgaagaacat cggcggatac tttctgagat ccttgatgaa
     1681 ggtcgggaaa tcgcgcggat tggcgatcaa caggccggtg ccacccagcg ctgcaaatag
     1741 ccagcagttc gcggttagtg agaagatgtg atagagcggc agcgcacaca cgaaggtggt
     1801 gcgctccatg ggcagctgct gaaaccacgc gcgtgcctgc aggatgttgg ccaccatatt
     1861 gccgtgcgtg agcatcgcgc ccttggccac accggtcgtg ccgccggtgt actgcagata
     1921 ggcgaggtca ttgtgatcca gcgtcactgg ccgcagcgcc tggcgctggc cttgactgat
     1981 cacctggttg aatcgccggg cgctgcggat cgcgaacgtc ggcacctgct tgcgtacgtg
     2041 gcgcaggacg aagtttgcga tcgatcccat cggccagctc aggcgatcgc cgattgccgt
     2101 cacgatcaca cagcgcacgc tggtgcgtgg caatacctgt tgcagggtgg cagcgaaatt
     2161 ctcgagcacc accaccgcgg cggcgcctga atcgttcaac tgatgctcca gctccgcggc
     2221 ggtgtacaac ggattggtgt tgacgattgt cagacccgcg cgcagcgcgc cgaacattgc
     2281 gatgggatac tgcagagtgt tcggcagcat gatggcgatg cgatcaccac tcttgagtcc
     2341 cgcctgcagt tgcagccagg cgccgaacga gcgcgagtca cgttccatcc gcgcgtaagt
     2401 cagcgccgtg cccatactgc ggtaggcaat acggtcggcg tagcgctggc aggcgtcttc
     2461 gatcagggct ttgagcgagg cgaattcatg cgggtcgata tccgccggta cgccgggtgg
     2521 gtactcgcgc agccagaacc tgtccatcgt gaatatccct taccccttgt agtgcgcgcc
     2581 gatcagggct tgcgtgagcc gagcaagggt tcgagtttgg gccacaccgt gtccagcaac
     2641 aacggttccg cgggttcggt cgggtggata ccgtcggcct gcatcagggt cggcgtgagc
     2701 gccacttttt cgagcaggaa cggaaccagt ggcaattggt actgctgcgc gaggtcggta
     2761 tacatcttct gaaacccgct ggtgtagcgc tcgccgtagt tcgggggcat tcgcatgccg
     2821 accagcacta cttggacccc ttgagcgcgt gccagctgga tcattttgtc aagattgtcg
     2881 tgcgtcgtct ccagcggcag gctgcgcaaa ccgtcgttgc cgccaagctc cagaatcaag
     2941 atgttcggat gctggacctc gagcgtgtgc ggcagacggc ccagcccgcc cgcggtggtc
     3001 tcaccgctga cgctggcgtt tacgacccta aatccgtaac ctaattgcgt gatcttctgc
     3061 gccagcaggt tcacccagcc gtgctcgacg cgaataccgt aacccgcgct gatgctgtcc
     3121 ccgaacacca agatgaccgg ttcgtggccg agcgcggccg gcgtcgtgct ggagccggca
     3181 gcgagcgccg tcgggaccgc gacggcgacc cacagtgcca acaggatgcc catgaagcgt
     3241 gacatcgttc tggaagcccg aaacctcacc aagcaggtta acagccccga agggccgctg
     3301 accattgtgc gcgatatttc attcagtgtg acgcagggtg aatccgtggc tctggtcggg
     3361 ccgtccgggg ccggcaagtc cacggtgctg gcgttgcttg cgggactgga tctgcctagc
     3421 tctggagagg tcgtgctctc ggggcaaacg ctgagctcac tgaatgagga cggacgcgcc
     3481 aagttacgcg ccgagcgggt cgggttcgtg ttccagtcgt tccacctggt gccgtcactg
     3541 acggcgctcg agaatgtgat gctgccgttg gaactgagcg gccgcagcga tgcgcgcgag
     3601 cgcgcgacgc agatgctgtc gcgcgtcggc ctcaataggc gtcttggtca ctacccgcgg
     3661 cagctttccg gcggcgagca gcaacgcgtg gcgctggcac gcgcctcggt cggcccaccg
     3721 gcggtgctgt tcgccgacga gccgaccggc aatctggaca gcgtcaccgg gga
//
LOCUS       DATMRM010000351        13928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2681795, whole genome shotgun sequence.
ACCESSION   DATMRM010000351 DATMRM010000000
VERSION     DATMRM010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13928
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2681795"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..624)
                     /locus_tag="VNZ06_10505"
     CDS             complement(46..624)
                     /locus_tag="VNZ06_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21226.1"
                     /translation="MLAHAIDTKVSPASRPLRQPKHNALEALFRTALSGGDGLTGAHC
                     VHELWMRGEMPVNIEHALEQLWKHAANSIPHWLPMRHIQWLPLAYEVAARFRADGGRN
                     NIYLVLLDYADRGGEPHGVYVGMSHYAPAVRFDQHKAGIRAAGSVLKRGLEVLTGPTL
                     HLQHIERVQAARIERQLAEALRDEGLLVQGGH"
     gene            819..1235
                     /locus_tag="VNZ06_10510"
     CDS             819..1235
                     /locus_tag="VNZ06_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21227.1"
                     /translation="MIKTFSLYEAATGLFTGKRLQCDEDGLVEARLPPGTLAIEGAFD
                     QMSQRVDLATGKIVAYHPLAPSPDHEWNAASKRWQVKKGARESEGRRAVVFARIHALE
                     TSQGRAIREATLGDPAAMKRLKEIDAEIAALRAELQ"
     gene            1304..2524
                     /locus_tag="VNZ06_10515"
     CDS             1304..2524
                     /locus_tag="VNZ06_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiA/YebS family transporter subunit"
                     /protein_id="HWW21228.1"
                     /translation="MSQPHPLHSAELIGCADCGRVQRVPPSERDVVPECACCGHEFAP
                     PTSGNFNAAQAYAVAALLLLLPPFLSPLLTINSFGISRHDWFPSGVEAMWDDGFGSLA
                     TLVFLFSIVFPFLYLSLMVWVLTSLQLGVTAWVGRIYRYAGWLRPWLMIEVYLVGCCV
                     AYTRLEQVGTVTIGLGGWCLIGACIAWILLALKLDARMIWETLPSAPPPAPGAATISC
                     LTCELLVDARRENSRCPRCSARLWKRKPDSFRRTTALVITGYLLYLPANLLPVLTIDR
                     FGRQTPSTILGGVHELIINNLWPLAIVVFSASIIVPLMKLLGLTLMLVQTQMRSTRWL
                     LFRTRLYRLIDMIGRWSNIDLFMISLLVALVQFGNLTTVRPQPGAIAFAAVVVITMIA
                     SRCFDTRLMWDATD"
     gene            2529..4148
                     /locus_tag="VNZ06_10520"
     CDS             2529..4148
                     /locus_tag="VNZ06_10520"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWW21229.1"
                     /translation="MADNDQNVSNAILRPRRWFSWVWVAPLGAAAIVIGLAIRGLEDR
                     GPLVTISFTEAEGLQPGETKIRHKDVELGTVERVYLTRDMSRVMVEARMRRSVTEHLN
                     EGSRFWIVRPRVGIGGISGLSTLVSGAYIEMYPGDGPSERNFVGLDEPPTLQPDAPGH
                     SFTLRAADLGQVVEGSPVDYRGVPVGEVEGFALDPSSKQIEIYAFVRAPYEHLVHPET
                     RFWNSGGVDVSIGPQGLRFRAESWQQLLSGGVSFDTPDTALEGTPSPEGSVFRLYANQ
                     SDAYEDPRGPTVVYRAIFRGASGNLGPGSAVELQGVEVGQVISAQLQYDDISGLPQVL
                     TTLQVDPGRIQIVHPQGDSHATATDALRARIAKLVTHGLRAELITASFITGNKVVSLD
                     IHKDAPAAKIELVDGLPQLPSVSGSDLTQILANVKSVLHHIDAATEGPELGHAIRELD
                     RTLTNLDQLTHEVQPQIQPLLQSLHDTAEATQRTLQSADRMLGGRASSNGDLGHLLRE
                     LTDAARSLRALTDYIDRHPESLIRGRQGETP"
     gene            4145..4729
                     /locus_tag="VNZ06_10525"
     CDS             4145..4729
                     /locus_tag="VNZ06_10525"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HWW21230.1"
                     /translation="MIRLRDTVLGLLACGALLCCGSSPPTRYYALNEIAPESASNASA
                     LEGAIPVRLEPVAIPSELDRPELVNHFGPNQVRFADLDRWAAPLDEQIRRTLSDDLSA
                     RLPAGLVADPNEPNTREPRRTLSISIGQFSADATCALTLNASWLLQRPHASAQSGEER
                     IQQPSAGACPAGLPAAMSRALAILADRLVPVLAR"
     gene            complement(4752..5354)
                     /locus_tag="VNZ06_10530"
     CDS             complement(4752..5354)
                     /locus_tag="VNZ06_10530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWW21231.1"
                     /translation="MQDFAARTLQYYRERAQEFWAGTRDHDVGQNIDALLRSIECAAP
                     FSILDFGCGPGRDLRAFAALGHTVIGLEGADEFVAMARESGCEVWQQDFLQLQLPPTH
                     FDGVFANASLFHVPSAQLPRVLRELHASLKARGVLFSSNPRGQNEEGWTGERYGVYHD
                     LAAWRGFLNSAGFTEVDHYYRPPGVPFERQRWLASVWRKS"
     gene            complement(5363..5734)
                     /locus_tag="VNZ06_10535"
     CDS             complement(5363..5734)
                     /locus_tag="VNZ06_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21232.1"
                     /translation="MKAIVAMVAIAGLCSTAAFADCPYPSGPKSIPDGNKATLDEMLT
                     MKKAVKQYDDDTANYLTCIQREHDAKLAELGEKVTDKQKADLDRIEADRHNAAVAQLQ
                     GVADKFNEQVRVYKAKNDKKP"
     gene            complement(5844..6326)
                     /gene="rraA"
                     /locus_tag="VNZ06_10540"
     CDS             complement(5844..6326)
                     /gene="rraA"
                     /locus_tag="VNZ06_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005251595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HWW21233.1"
                     /translation="MTQSTSDLYDHYGERLRVMPPVFQDFGGRTQFSGEVVTVRCFED
                     NSRLKELLSTPGAGKVLLVDGGGSQRCALMGDLIAGQAVTHGWEGVVIYGCVRDRIAL
                     KALNLGIRALGATPRKSLRRGEGQTQLPIEIGGVSCRPGDRLVADEDGIVLLDPNSSA
                     "
     gene            6366..7856
                     /locus_tag="VNZ06_10545"
     CDS             6366..7856
                     /locus_tag="VNZ06_10545"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWW21234.1"
                     /translation="MPRSAYNVESPGDRRRELVRTLRSQLPGDCVLSSAEELRPYECD
                     GLTAFRQPPIAVVLPRDEAQVCTALRLCREGSVPVVARGAGTGLSGGAMPHAEGIVLS
                     LARFDRILEIDPIGCTARVQPGVRNLAVSEAAAPHGLYYAPDPSSQIACTIGGNVAEN
                     SGGVHCLKYGLTVHNVLGVRGYSIEGELIELGGYSLDAPGLDLLALVLGSEGLLMLVT
                     EVILKLIPRPPLAQVVMASFDDVSRAGEAVAAIIAAGIIPAGLEMMDRKATEAVEPFV
                     RAGYDLKAEAILLAESDGTPDEVRDEVATMERILRAAGATACAVSGNEAERLRFWSGR
                     KNAFPAAGRVSADYYCMDGTIPRKSLGAMLKAIEEMERTYSLKCMNVFHAGDGNLHPL
                     ILFDANRPGEWERADRFGADILELCIKLGGTITGEHGVGIEKINSMCVQFSPAERAAF
                     FAVKSAFDPPGLLNPGKAIPTLRRCAEYGRMHVHRGQLPHPELPRF"
     gene            7858..8919
                     /gene="glcE"
                     /locus_tag="VNZ06_10550"
     CDS             7858..8919
                     /gene="glcE"
                     /locus_tag="VNZ06_10550"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008439.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HWW21235.1"
                     /translation="MDAILQDLGARIADAHASATPLRLRGAGTKDFYGEQVEGELLCL
                     RAYADVVDYEPSELVITARCGTPLSQVERLLAQHGQYLAFEPPAFGSDPTIGGVIAAG
                     LSGPQRARAGAARDFVLGAMLLGIDGKALHFGGKVMKNVAGFDVSRLLCGSLGILGPI
                     VEVSLKVQPLPRAQQTLCFDLSADQALSAFNRWNSQPLPISACAWHDARAWVRLAGTE
                     SALRTARERLGGEALEPERASQWWLSVRHQRHAFFEGAASLWRLSLPSSCASLPLSAS
                     PLIEWGGALRWYASDLPPASMRKIASAAGGSALHWRGGAAGARFHPLSASVRGLHRRL
                     KQQFDPHRIFNRGRLLADL"
     gene            8939..10198
                     /gene="glcF"
                     /locus_tag="VNZ06_10555"
     CDS             8939..10198
                     /gene="glcF"
                     /locus_tag="VNZ06_10555"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008434.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HWW21236.1"
                     /translation="MQTRLLPDLKDSVDGQAAEAILRACVHCGFCNATCPTYQLLGDE
                     LDGPRGRIYQIKQVLEGTPATPITQLHLDRCLTCRNCESTCPSGVEYGHLLDIGRCAV
                     EAQVKRPVRQRLLQALLRIGLSHRTLFGVAMRVGRALRSLLPAALRAKLQPARPPGPW
                     PRRLHTRRVIALRGCTQPAMLPSIDAATARVLDALGIQTLIAASAGCCGAIHQHLNDH
                     AGALDHMRRNIDAWWPLLSAGGAEAIIINASGCAVTVKEYGYLLRQDPAYAEKARRIS
                     QAARDLSEYLLPEICALRARIAAPAGQRIALQIPCTLQHGQKLAGTLESLLQGLGVQM
                     DPVAEAHLCCGSAGTYSLLQSELSRSLRDRKLGNLQQSQPQIILSANIGCIAHLQTGT
                     TLPVWHWIEWLDQSLQPATGSASGNRP"
     gene            complement(10181..11629)
                     /locus_tag="VNZ06_10560"
     CDS             complement(10181..11629)
                     /locus_tag="VNZ06_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HWW21237.1"
                     /translation="MDLDHDACYRALRLRDARFDGRFFTAVKTTGIYCRPICPARCPK
                     SKNVIFYPTAAAAQEAGFRPCLRCRPETAPDLGAWRGTSNTVARALRLIEMGALDDAS
                     IDALALRLGVGERQLRRLFRQHLGASPVSVAQTRRVLLAKQLLHETRMPMAEIAFASG
                     FGSIRRFNEIFQSLYHRAPSELRRARQPDVSVGAEGELTLLLRYRPPYDWEAMLQFLR
                     RRAIAGLEEVTERSYARSIQFDGVQGTVLVEPTDSAALRARIRLPALTALPKIIARLR
                     RVFDLAADTDAIAAHLARDPALAPLIRARPGLRVPGAWDGFELAVRAVLGQQITVIGA
                     ARIAARLVEQYGEPMARPVGSITRVFPLPQALAGHELEGLGVPRSRANTLSGLAAAVV
                     ADPHFFDANRDLSEAIAQLRAVKGVGDWTAQYIALRQLREPDAFPHGDVALIRALQAR
                     EGLRRTSSELLSRADVWRPWRAYAAQHLWSVA"
     gene            11769..12467
                     /locus_tag="VNZ06_10565"
     CDS             11769..12467
                     /locus_tag="VNZ06_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HWW21238.1"
                     /translation="MIDVHYWTTPNGHKVTMFLEEAGVPYRLFAVNIGAGDQFKPEFL
                     KISPNNRIPAIVDNEPKPGGAPISVFESGAILLYLAEKTGKFIPSDIRGRVETLQWLF
                     WQMGGLGPMLGQNHHFGLYAPEKIPYAIERYVKETNRLYGVLNKRLADREFVAGPYTI
                     ADMAAYPWIVPYERQGQKLEDFPHLKRWFEAIRERPAVQRAYARAKEINSAPTMSEEA
                     KKVLFGQTAASVAR"
     gene            complement(12464..12763)
                     /locus_tag="VNZ06_10570"
     CDS             complement(12464..12763)
                     /locus_tag="VNZ06_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21239.1"
                     /translation="MRRFDRSAKLKDPLRLISCGNCQRLMDFMDRPGHPPPATDPDKL
                     HPIFLKNPPAYSVLCTCGHYTFYVDEADRAHYEAKYFKSDRASAGSPAGDEPASR"
     gene            13006..13773
                     /locus_tag="VNZ06_10575"
     CDS             13006..13773
                     /locus_tag="VNZ06_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HWW21240.1"
                     /translation="MEIADRVFIVTGGASGLGEAVVRHLAARKARVLILDLDEARGRA
                     LVQELGAQTGFARTDVGSEVDVQAAVDRAMRDFGGLHGAVNCAGIATSVKVLGKEGPG
                     KLADFARVININLIGTYNVIRLAAHGMSSQAPNADGERGVLINTASVAAYDGQIGQPA
                     YAASKAGVVGLTLPVARELARFGIRCVTIAPGLFATPMMESLPPEVKEVLGKQVPFPA
                     RLGRPHEYALLVQQIIENPMLNGETIRLDGALRMPPK"
BASE COUNT         2441 a         4509 c         4489 g         2489 t
ORIGIN      
        1 cagccaaccc agagcagccc cgcgcatgcg caggtttctc atgagtcagt gccctccctg
       61 gacgagcaag ccctcatcgc gcagcgcttc ggccagctgc cgttcaatgc gcgccgcctg
      121 cacacgttcg atgtgctgca ggtgcagggt cggcccggtc agtacctcca gcccacgctt
      181 gagtacactg cccgcagcgc gaatgcccgc cttgtgctga tcgaaacgca cggccggcgc
      241 atagtgactc atgccgacgt agacgccatg tggctcgccg ccgcgatcgg cgtagtcgag
      301 cagcaccaga tagatgttgt tgcggccgcc gtccgctcga aagcgcgccg ccacctcata
      361 ggccaggggc agccattgta tgtggcgcat cggcagccag tgaggaatgg agtttgcggc
      421 gtgcttccac aactgctcga gcgcatgctc gatattgacc ggcatctctc cgcgcatcca
      481 cagttcgtgc acgcaatgcg cgcccgtcag accgtccccg ccggacagcg ccgtgcgaaa
      541 cagtgcctcg agggcgttgt gcttcggctg gcgcagcggc cgtgaggccg gcgacacttt
      601 ggtatcgatc gcgtgcgcaa gcatgccggg ttcctaaata atgagataac gtttacatcg
      661 taagcttttg ttttatatta ttgacgcaat ggacctagca ccgctcaaaa acccgacgtg
      721 tgacgtccgt cgattcgtag cccgtgaacc ctgtcgcata catcaggcga gtttcggtct
      781 taactattct cggacgattc tgtggatctg atcggtacat gattaagaca ttcagcctgt
      841 acgaggcggc taccgggttg ttcaccggca agcgtctgca gtgcgacgaa gacggactgg
      901 tggaagcgcg gcttcctccg ggcacgctgg cgattgaagg ggccttcgac cagatgagtc
      961 agcgcgtgga cctggctacc ggcaagatcg tcgcctatca cccgctcgcg ccctcgcctg
     1021 accatgagtg gaatgccgcc agcaagcgct ggcaggtcaa gaaaggcgcg cgcgagtccg
     1081 aaggtcgccg cgcggtggtg tttgcgcgca ttcatgcgct cgaaaccagc cagggccgtg
     1141 cgatccgcga ggccacgctc ggtgatccgg cagcgatgaa acggctgaag gaaatcgacg
     1201 ccgaaatcgc cgcgctgaga gccgagctgc agtaagcgct tcccggtccc gcctgcggcg
     1261 gtgacagcgt atagtccgcg tccatgacac ccgctgtgga cgcgtgagcc agccacatcc
     1321 ccttcatagt gccgaactca tcggttgcgc cgactgcggc cgggtgcaaa gagtaccgcc
     1381 ctccgagcgt gacgtggtac ccgagtgcgc ctgctgcggt cacgaatttg cgccgccgac
     1441 gagcggcaat ttcaatgcgg cccaggccta tgcggtcgca gcgctgctgc tgctgttgcc
     1501 gccgtttctg tcgccgctgc tgacgatcaa ctcgtttggt atctcacgcc acgactggtt
     1561 ccccagtggg gtcgaggcga tgtgggatga cgggttcggc tcgctcgcca ccctggtgtt
     1621 cctgttcagc atcgtcttcc cgttcctgta tctgtcgctg atggtgtggg tgttgacgtc
     1681 actgcagctc ggcgtcaccg cctgggtcgg caggatctac cgctacgccg gctggctgcg
     1741 gccgtggctc atgatcgagg tctatctggt gggctgctgt gtggcctata cgcgtctgga
     1801 gcaggtcggc accgtgacga tcggactggg cggatggtgc ctgatcggcg cctgcatcgc
     1861 ctggatcctg ctggcgctga agctcgacgc gcgcatgatc tgggagaccc tgcccagcgc
     1921 gccgccaccc gcgcccgggg cggcgacgat cagctgcctg acctgcgagc tgctggtgga
     1981 cgcgcgacgg gagaattcgc gctgcccgcg ctgtagcgcc cgcctctgga agcgcaaacc
     2041 cgattccttc cgacgcacca ccgcgctggt gatcaccggc tatctgctct atctgccggc
     2101 gaacctgctg ccggtactca cgatcgatcg tttcggacgc cagacgccga gcacgattct
     2161 cgggggcgtg cacgagctga tcatcaacaa cctgtggccg ctggccatcg tggtgttcag
     2221 tgccagtatc atcgtacccc tgatgaaact gctcggactg acgctgatgc tggtgcagac
     2281 gcaaatgcgt tcgacgcgct ggctgttgtt ccgcacgcgc ttgtatcgtt tgatcgatat
     2341 gattgggcga tggtcgaaca tcgatctgtt catgatctcg ttgctggttg cgctggtgca
     2401 gttcggaaac ctgaccacgg tccggcctca gccgggcgca attgcctttg cggccgtcgt
     2461 ggtcattacg atgatcgcct cgcgctgctt tgatacgcgc ctgatgtggg acgccaccga
     2521 ctagcaccat ggccgacaac gatcagaacg tttccaacgc gatactgcgc ccgcgccgct
     2581 ggttctcgtg ggtgtgggtc gcgccgctgg gcgccgcggc gattgtcatc gggctcgcca
     2641 tccgcggact ggaggatcgt ggtccgctgg tcacgatctc attcactgag gccgaaggcc
     2701 tgcagcccgg tgagaccaag atccgacaca aggatgtgga gctcggcacg gtcgaacgcg
     2761 tttatctgac gcgcgacatg tcacgcgtca tggtcgaagc gcgcatgcgc cgctcggtta
     2821 ccgaacacct gaacgaaggc tcacgcttct ggatcgtgcg accgcgcgtc ggcatcggcg
     2881 gtatctccgg actgtccaca ctggtctccg gcgcgtacat cgaaatgtat ccgggcgacg
     2941 gcccgtcgga gcgcaacttt gttggcctgg acgaaccgcc aacgctgcag ccggatgcgc
     3001 cgggacattc atttaccttg cgggcggccg atctgggaca ggttgtcgaa ggctcgccgg
     3061 tggactaccg gggtgtcccg gtcggtgagg tcgagggatt cgcgctcgac ccgagttcca
     3121 agcagatcga gatctatgca ttcgtacgcg cgccgtatga gcacctggtg cacccggaga
     3181 cgcgcttctg gaactccggt ggagtggacg tctcgatcgg tccacagggc ctgcgatttc
     3241 gcgccgagtc ctggcagcaa ttgctgtccg gcggcgtgag tttcgatacg cccgacaccg
     3301 cgctggaggg cacgcccagc cccgaaggct cagtgttccg tctatacgcc aatcaaagcg
     3361 atgcgtacga ggatccgcgt ggacccaccg tggtctatcg cgcaattttc cggggggctt
     3421 ccggcaacct cggtcccggc agtgcagtcg agcttcaggg tgtcgaggtc gggcaggtca
     3481 tcagcgcgca actgcaatac gacgacatca gtggcctgcc gcaggtgctc accacgctgc
     3541 aggtcgatcc gggccgcatc cagatcgtgc atccacaggg cgacagccat gccacggcga
     3601 ccgatgcgct gcgagcgcgc atcgcgaagc tcgtcacgca cggcctgcgc gcggaactca
     3661 tcaccgcgag cttcatcacc ggcaacaagg tggtctcgct cgacatccac aaggacgcgc
     3721 ccgcggccaa gatcgaactg gtcgacggcc tgccgcagct gcctagtgtc agcggctccg
     3781 acctgacaca gatcctggcg aatgtgaaga gcgtgctgca tcacatcgat gccgccaccg
     3841 agggtccgga actcggacat gccatcaggg aactggatcg gacgctgacc aatctggatc
     3901 agctcacgca tgaggtgcag ccgcagattc agccgttgct gcagagcctg cacgatacgg
     3961 ccgaggccac tcagcgcaca ctgcagagcg cggatcggat gctcggcggg cgagcctcca
     4021 gcaacggtga tctgggccat ctgctgcgtg aactgaccga tgccgcgcgc tcgcttcgag
     4081 cactgaccga ctacatcgat cgtcatccgg aaagcctgat tcgcggccgc caaggagaga
     4141 ccccatgatc cgcctgcgcg acaccgtgct cggcctgctc gcctgcggcg ccctgctctg
     4201 ctgtgggtcc agtccgccga ctcgctacta cgcgctcaat gaaatcgcgc ccgagtcagc
     4261 ctccaacgcc agtgcgctag aaggcgcgat cccggtgcgt ctggagcccg tggccatccc
     4321 ttcagagctc gatcggcccg agctggtcaa tcatttcggg cccaatcagg tgcgcttcgc
     4381 cgacctggac cggtgggccg cgccgctgga tgagcagatc cgccgcacgc tgtcggatga
     4441 tctgtcggct cgattgccgg ccggcctggt ggccgatccg aacgagccga atacacgcga
     4501 accacgccgc acgctgtcca tatcgatcgg gcagttcagt gccgatgcca cctgcgcgct
     4561 cacgctcaat gccagctggt tgctgcagcg accccatgcc agcgctcaga gcggggagga
     4621 gcgcatccag cagcccagtg ccggcgcatg cccggcgggg ctgccggcgg cgatgagccg
     4681 cgctctggcg atactggccg atcggctggt accggtgctg gcgcgatagt cgggggcgct
     4741 cggcggggga tctaggactt gcgccagacg ctggccagcc agcgctgccg ctcgaacggc
     4801 acgccgggcg gacggtagta gtgatcgact tccgtgaacc cggctgagtt caggaagccg
     4861 cgccacgcgg cgaggtcgtg atagacgcca tatcgctcgc cagtccaccc ttcctcgttc
     4921 tgaccacgcg ggttggaact gaacaacaca ccccgcgctt tgagcgacgc gtgcagttcg
     4981 cgcagcaccc gtggcagctg cgcgctgggt acgtgaaaca gcgaggcgtt ggcgaacacg
     5041 ccgtcgaaat gtgtcggcgg cagctgcagc tgcaggaaat cctgttgcca gacttcgcat
     5101 ccggattcac gcgccatggc cacgaactca tcggcccctt ccaggccgat gacagtatga
     5161 ccgagcgcgg cgaacgctcg caggtcacgc cccggtccgc aaccgaagtc cagaatgctg
     5221 aagggagcag cgcactcgat gctcctcagc agcgcatcga tgttttgccc gacgtcgtgg
     5281 tcacgggttc ccgcccagaa ctcctgcgcg cgctcccggt agtactgcaa ggtacgcgca
     5341 gcgaagtcct gcacggcgcg cgctagggtt tcttgtcgtt cttcgccttg tagacgcgga
     5401 cctgttcatt gaacttgtcg gcgacaccct gcagctgcgc gaccgccgca ttgtgccggt
     5461 cggcctcaat gcgatcgagg tccgctttct gcttatccgt caccttctcg ccgagctcgg
     5521 ccagcttggc atcgtgctcg cgctgaatgc aggtcaggta gttggcggtg tcatcgtcgt
     5581 actgtttgac ggccttcttc atcgtgagca tctcgtcgag tgtcgccttg ttgccgtccg
     5641 gaatcgactt cggccccgaa ggatacgggc agtcggcaaa cgccgctgtg ctgcagagac
     5701 ctgcgatggc gaccatcgcc acgattgctt tcataccctt agcttccccg gttgttattt
     5761 agacaattat tctagtgcga gcgccctgcc ccggccaact tctttgaagt ttaatccggg
     5821 cggacggctc gccggccgcg atgtcaagca gaggaattcg gatccagcag tacgattccg
     5881 tcctcgtcgg ctaccagccg gtcaccggga cggcagctga cgccgccgat ctcaatgggg
     5941 agctgggtct ggccctcgcc gcgccgcagg gatttgcgcg gtgtggcccc gagtgcgcgg
     6001 atccctaagt tcagcgcctt gagggcgatg cggtcgcgca cgcagccgta gatcacaacc
     6061 ccttcccaac catgggtcac ggcctgaccg gcaatcagat cacccatcag cgcgcagcgc
     6121 tgactgccgc ccccgtccac cagcagtacc ttgccggcac cgggtgtgct gagcagttcc
     6181 ttcaggcgcg agttgtcctc gaaacagcgc accgtgacga cctcgccgct gaactgcgtc
     6241 cggccgccga agtcctggaa caccggtggc atcacacgca ggcgttcgcc gtagtggtca
     6301 tacagatctg aggtggattg cgtcatcgaa agtagccccg ccgtaataac tgttaggatt
     6361 tgagcatgcc gcgctctgct tacaacgtcg agtcgcccgg cgaccggcgg cgcgagttgg
     6421 tccggacact gcgctcgcag ctgccgggtg actgcgttct gagcagcgcg gaggaactgc
     6481 gaccctatga atgcgacggg ctgacggcat tccgtcagcc tccgatcgcc gtggtgctgc
     6541 cgcgcgatga agcgcaggtg tgcacggcac tgcggctgtg ccgcgagggc tcggtgccgg
     6601 tggtggcacg tggagcgggc accggactgt ccggcggcgc catgccgcac gccgagggta
     6661 tcgtgttgtc cctggcgcgc ttcgatcgca ttctggaaat cgatcccatc ggctgcaccg
     6721 cgcgcgtgca accgggtgtg cgcaacctgg cggtctcgga agcggcagcc ccgcatggcc
     6781 tgtactacgc gcccgatccg tcttcccaga tcgcgtgcac catcggcggc aatgtggccg
     6841 agaattccgg cggcgttcac tgcctgaaat acggcctgac cgtgcataac gtgctcggcg
     6901 ttcgcggcta tagcatcgag ggcgagctca tcgagctcgg cggatattca ctggatgctc
     6961 cgggtctgga tctgctggca ttggtgctcg gctctgaggg actgctgatg ctggtcaccg
     7021 aggtgatcct gaagctgatc ccgcgaccgc cactggcgca ggtcgtgatg gcctccttcg
     7081 acgacgtcag cagggccgga gaggcggtcg ccgccatcat cgcggccggc atcattccgg
     7141 cgggtcttga gatgatggat cgcaaggcca ccgaggccgt cgaacctttc gttcgcgcgg
     7201 gctacgacct gaaagccgag gccatcctgc tggccgaatc ggacggcacc ccggatgaag
     7261 tgcgcgatga agtcgccaca atggaacgga tcctgcgcgc cgcaggggcc accgcctgcg
     7321 cggtctccgg caacgaggcc gagcgcctga gattctggtc cggacgcaag aacgcatttc
     7381 ccgccgccgg gcgcgtctcg gccgactact actgcatgga tggcaccatc ccgcgcaaga
     7441 gtctgggtgc gatgctcaag gcgatcgaag aaatggagcg tacctactcg ctcaagtgca
     7501 tgaatgtgtt ccatgccggc gatggcaatc tgcatccgct gatcctgttc gatgccaacc
     7561 gccccggcga gtgggaacgc gccgaccgtt tcggcgccga catcctggaa ctgtgcatca
     7621 aactcggcgg cacgatcacc ggcgagcatg gcgtgggtat cgagaagatc aattccatgt
     7681 gcgtgcagtt ctcgccggcc gaacgcgcag cgttttttgc cgtgaaaagc gcgttcgatc
     7741 cgccggggct gctcaatccc ggcaaagcga tcccgacact gcggcgctgc gccgagtatg
     7801 gccgcatgca tgtgcatcgc ggacagctgc ctcatcccga gctgccgaga ttctaggatg
     7861 gacgcgatac tgcaggatct gggggcacgc atcgccgacg cccacgccag tgctacgccg
     7921 ctgcggctgc gcggcgcggg aaccaaggat ttctacggcg agcaggtgga aggcgaattg
     7981 ctttgcctgc gcgcctacgc cgacgtcgtg gactatgaac cctcggaact ggtcatcacc
     8041 gcgcgctgtg gtacgccgct gtcacaggtc gagcggctgc tggcgcagca cggacagtat
     8101 cttgcattcg agcctccggc cttcggcagc gatccgacga ttggcggcgt cattgctgcg
     8161 ggactgtccg gcccgcaacg cgcacgcgcc ggggccgcgc gcgatttcgt gctgggtgcg
     8221 atgctgctcg ggatcgacgg caaggcactg cacttcggcg gcaaggtcat gaagaacgtc
     8281 gccggcttcg atgtctcgcg gctgctgtgc ggttcactgg gcatcctggg tcccattgtc
     8341 gaagtgtcgc tgaaagtgca gccgctgccg cgcgcgcaac agacgctgtg ctttgatctg
     8401 agtgccgatc aggccctgag cgccttcaac cgctggaatt cgcagccgct gccgatcagc
     8461 gcctgcgcct ggcatgacgc acgcgcgtgg gtgcggctcg cgggcaccga gtccgcgctg
     8521 cgcacggcgc gcgagcggct ggggggcgag gcgctcgagc ccgagcgagc ctcgcaatgg
     8581 tggctgagcg tgcgacatca gcgccatgcc ttctttgaag gcgcagcgtc gctgtggcgt
     8641 ctgtcgctgc cctccagttg cgcctcgctg ccgctatccg cctcgccctt gatcgaatgg
     8701 ggtggcgccc tgcgctggta cgcgagcgat ctgccgccag cctcgatgcg caagatcgcc
     8761 agcgctgcgg gcggctcggc gctgcactgg cgcggaggcg cggcgggcgc gcgtttccat
     8821 ccgctgtcgg ccagcgtgcg cggcctgcat cgccggctga agcagcagtt cgatccgcac
     8881 cggattttca atcgcggccg actgctcgcg gatctctaga caccgctcaa ggcaccgcat
     8941 gcagaccaga ctgctccccg atctcaagga cagcgtcgac ggtcaagccg ccgaggcgat
     9001 cctgcgcgcc tgcgtgcact gcggcttctg caacgcgacc tgtcccacct atcagctgct
     9061 tggggacgaa ctggacggac cgcgcgggcg catctatcag atcaagcagg tgctcgaggg
     9121 cacacccgcg acaccgatca cgcagttgca tctggatcgc tgcctgacct gccgcaactg
     9181 tgagagcacc tgcccgtccg gcgtggagta cgggcatctg ctggatatcg ggcgctgcgc
     9241 ggtggaagcg caggtgaagc gtccggtcag gcagcgcttg ttgcaggcgc tgctgcgcat
     9301 cggactcagc caccgtaccc tgtttggtgt ggcgatgcgc gtcggacgag cgctgcgcag
     9361 cctgctgccc gcggcgctgc gcgccaagct gcagccggca cggccgccag ggccctggcc
     9421 gaggcgcctg cacacccgcc gggtgattgc gctgcgcggc tgcacgcagc cggcgatgct
     9481 gccgtcgatc gatgccgcga ccgcgcgcgt gctcgatgcc ctcggtatcc agaccctgat
     9541 cgcggcgtcg gccggttgct gtggagcgat ccatcagcat ctgaatgatc acgccggtgc
     9601 gctcgaccac atgcgtcgca acatcgatgc ctggtggcca ttgctatcgg ctggcggtgc
     9661 ggaagcgatc atcatcaatg cctccggctg cgcggtcacg gtcaaggaat acggttatct
     9721 gctgcgccaa gatcctgcgt acgccgagaa ggcgcgtcgc atctcgcaag cggcacgcga
     9781 cctgagcgag tatctgctgc cggaaatctg cgcgctcagg gcgcgcatcg ctgcacctgc
     9841 cgggcagcgc atcgcgctgc agattccgtg cacgctgcag catggacaga agctggcggg
     9901 cacgctcgag tcgctgctgc agggattggg tgtgcagatg gacccggtgg ccgaagcgca
     9961 tctgtgctgt ggatcggcgg gtacctactc gctgctgcaa agcgaactgt cccgctcgct
    10021 gcgcgatcgc aagctcggaa atctgcagca atcgcagccg cagatcatcc tgtcggccaa
    10081 tatcggctgc atcgcgcatc tgcagaccgg cacgacgctg ccggtatggc actggatcga
    10141 gtggctcgat caatcactgc agcccgcgac tggcagtgcg tcaggcaacc gaccataggt
    10201 gctgggccgc gtacgcgcgc cacggccgcc acacgtcggc gcgcgacagc agttcggatg
    10261 aggtgcgccg caatccctcg cgcgcttgca gcgcgcgaat caacgcgaca tcgccgtgcg
    10321 gaaaggcatc cggctcgcgc agctggcgca gcgcgatgta ctgcgccgtc cagtcgccga
    10381 cacctttcac cgcacgcagc tgcgcgatcg cttccgacag atcgcgattc gcatcgaaga
    10441 aatgcggatc cgccaccacg gccgccgcca ggccgctcaa ggtattcgca cggctgcgcg
    10501 gcacgcctaa gccctcgagt tcgtgtccgg ccagcgcctg cggcagcgga aagacacgcg
    10561 tgatcgatcc caccggacgc gccatcggct cgccatattg ctccaccagc cgtgccgcga
    10621 tgcgcgcggc tccgattacc gtgatctgtt ggccgagcac cgcgcgcacc gccagctcga
    10681 acccatccca ggcgccgggc acgcgcagac ccggacgcgc ccggatcagc ggcgccagtg
    10741 ccggatccct ggccagatgc gccgcgatcg catcggtatc ggccgccaga tcgaacacgc
    10801 gccgcaggcg cgcaatgatc ttcggcagcg cagtgagcgc gggcaagcgg atcctcgcgc
    10861 gcaacgccgc ggaatcggtg ggctcgacca gcaccgttcc ctgaacgcca tcgaactgga
    10921 tcgagcgcgc gtagctgcgc tcagtgactt cctcgaggcc ggcaatcgcg cgccgacgca
    10981 ggaactgcag catcgcctcc cagtcatacg gcgggcggta acgcagcagc agcgtgagtt
    11041 caccctccgc accgaccgac acgtcgggtt gacgtgcacg ccgcagttca ctcggtgcgc
    11101 ggtgatacag cgactgaaag atttcattga aacggcgaat actgccaaag ccggacgcga
    11161 atgcgatttc cgccatcggc atgcgcgttt cgtgcagcag ctgtttcgcg agcagcacgc
    11221 gccgggtctg cgccacgctc accggggagg cgcccagatg ctgccggaac aggcggcgca
    11281 gctgtcgctc accgacaccc aggcgcagcg ccagcgcatc gatgctggcg tcatccagtg
    11341 cgcccatctc gatcagccgc agcgcacgcg cgacggtgtt cgacgtgccg cgccaggcac
    11401 ccaggtcggg cgcagtctcc ggacggcagc gcagacaggg ccggaagccc gcttcctgtg
    11461 ccgcggccgc cgtgggatag aagatcacgt tcttcgactt cggacaccgt gccggacaaa
    11521 tcggccggca gtagatgccg gtggtcttca ctgcggtgaa gaagcgcccg tcgaagcgcg
    11581 catcgcgcag gcgtagggcc cgataacagg cgtcgtggtc aaggtccatg gggcctgagt
    11641 ctgcgatcgg gccaggaagc tgtctagcgg ttttcggaca tcattggcgc gacatgaaag
    11701 tatcaagcgg attcgagtac gctaggcaac tcccacgcac cccaaccgat agcttggagg
    11761 taacgataat gatcgatgtc cattactgga cgactccgaa cggccacaag gtcacaatgt
    11821 ttctggaaga agcaggcgtg ccgtatcgcc tgttcgcggt caatatcggg gccggcgatc
    11881 aattcaaacc agagttcctg aagatctcgc cgaataaccg aattccggcc atcgtcgata
    11941 acgaaccgaa gccaggcggt gcgccgatca gcgtgttcga atccggcgcg atcctgttgt
    12001 acctcgccga gaagaccggc aagttcattc catccgatat ccgtggccgc gttgaaaccc
    12061 tgcagtggct gttctggcag atgggcggcc tgggcccgat gctcggacag aaccatcact
    12121 tcggcctata cgcgccggag aaaatcccgt acgcgatcga gcgctatgtg aaagagacca
    12181 atcgcctcta cggcgtactc aacaagcgcc tggcggatcg ggagttcgtt gccggtccct
    12241 acacgatcgc ggatatggcg gcctatccgt ggatcgtgcc gtacgaacgg cagggccaga
    12301 agctggaaga ctttccgcac ctcaagcgct ggttcgaagc gattcgcgag cgcccggcag
    12361 tgcagcgtgc ctatgcgcgt gccaaggaaa tcaacagcgc cccgacgatg agcgaggaag
    12421 ccaagaaagt actgttcgga cagaccgccg ccagcgttgc gcgctagcgg cttgccggct
    12481 cgtccccggc gggcgagccg gcgctggcac ggtcagactt gaagtatttt gcctcgtagt
    12541 gagcgcgatc ggcttcatcg acatagaacg tatagtgccc gcaggtacac agcaccgagt
    12601 aggcaggcgg gttcttcagg aagatcggat gcagcttgtc gggatcggtc gctggcgggg
    12661 gatgtcccgg gcggtccatg aagtccatca ggcgctggca gttgccacaa ctgatcagtc
    12721 gcagcggatc cttgagcttg gcggaacggt cgaaccttcg catgggcggc gaatggtcag
    12781 tgcatcaaag cgacggaccg cgtcgccccc gccatccatt caacaggaag gcgacgacca
    12841 gcagcagtaa aacaatcgag acagtgaata aatccaaggt tatctccgcc gtagggacta
    12901 ccgtgcgaag aatcttgcgc tgcagccggc ggacccggtg ataggacgct ctctcaagga
    12961 gagtgcaaat ttgacctaca gtggcccgac gtttagcgtg acgccatgga aatcgctgac
    13021 cgagtattca tcgttaccgg aggcgcttcg gggctcgggg aggccgtggt ccggcatctc
    13081 gcagcgcgca aggccagagt gctgatactg gacctcgacg aggcgcgcgg acgtgcgctg
    13141 gtccaggaac tcggggcgca gaccggattt gcgcgcaccg acgtcggctc cgaggtggac
    13201 gtacaggccg cggtcgatcg agcgatgcgc gacttcggag gattgcacgg cgcggtcaac
    13261 tgcgccggga tcgctacgtc ggtcaaggtg ctcggcaagg aaggcccggg caagctcgcc
    13321 gatttcgcgc gcgtcatcaa catcaatctg atcggtacct ataacgtgat ccggctggcc
    13381 gcacacggca tgagcagcca ggcccccaac gccgacggag aacgcggtgt cctgatcaac
    13441 accgcttcgg tcgcggcgta cgacggacag atcggtcagc ccgcttacgc ggcctccaag
    13501 gccggtgtgg tcggattgac gctgccggtc gcgcgcgaac tggcgcgttt tggcattcgt
    13561 tgcgtgacga ttgcacccgg attgtttgca accccgatga tggagtcgct gccgccggaa
    13621 gtgaaagagg tgctcggcaa gcaggtaccc ttccccgccc gtctcggtcg gccgcacgaa
    13681 tacgcgctgc tggtacagca gatcatcgag aatccgatgc tcaacggcga gaccatccgg
    13741 ctggatggcg cgttgcgcat gccgccgaaa taaccgaagg ttcaatgctg aatcgggctg
    13801 tagccgtcgt gctgtagcag cccgatgacc ccgtcgcgac caatcaggtg cagcgcgccg
    13861 acgatcacca ggtagttctt gtcgtcgctg agcatctgct cgattctcgg caaccactgg
    13921 cgattgcg
//
LOCUS       DATMRM010000352        10236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_831535, whole genome shotgun sequence.
ACCESSION   DATMRM010000352 DATMRM010000000
VERSION     DATMRM010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10236
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831535"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VNZ06_10580"
     CDS             <1..524
                     /locus_tag="VNZ06_10580"
                     /inference="COORDINATES: protein motif:HMM:NF040065.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21241.1"
                     /translation="FFRRTNINALGQPRSWTDHLLWHHACHTVDLFQYQTDQRVSECY
                     ALQGPAHPTLGIAMDMSIGLKVPGGALCTLSLSFNNDGPLGTFFRYICDNGTYICRYD
                     DLFDGKNNQIDVSRVDVSMNGIELQDREFFAAIAQQREPNASVAQCLTAMATLDRLER
                     MLTASAASGARSA"
     gene            521..1546
                     /locus_tag="VNZ06_10585"
     CDS             521..1546
                     /locus_tag="VNZ06_10585"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWW21242.1"
                     /translation="MIIDCHGHYTTAPGELQAFRDAQIEAFKQAGDASSVQAPRIGDD
                     QLRESLEKAQLKLQRERGTDLTIFSPRASAMAHHIGNEAVSAQWTRICNDLIARVCGL
                     YPENFVGVCQLPQSPGVPPAHCAAELERCVRDLGFIGCNLNPDPSGGHWNSPPLTDRH
                     WYPLYEKMVGLDVPAMVHVSASCNPNFHATGAHYLNADTTAFMQFLTSDLFKDFPTLR
                     FIIPHGGGAVPFHWGRYRGLAQDLKRPPLEELLLKNVYFDTCVYHLPGIELLFKVVPV
                     ENIIFGSEMVGAVRGVDPRSGHYYDDTRRYVDALDLSAADRQRVYAGNARRVFPRLDA
                     RLKAAGH"
     gene            complement(1543..2202)
                     /locus_tag="VNZ06_10590"
     CDS             complement(1543..2202)
                     /locus_tag="VNZ06_10590"
                     /inference="COORDINATES: protein motif:HMM:NF006731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HWW21243.1"
                     /translation="MREIARAAAAELSALGECGVATVHEAQGRIGLLASYLRPIYRPV
                     SICGSAVTVLVPPGDNWMLHVAMEVCRPGDVLVVAPSSACEDGYFGDLLATSCVAHGV
                     RGLIIDAGVRDIATLTDMKFPVWSKAVFAQGTVKASLGAVNVPVVCAGARVEPGDAIV
                     ADDDGVVVVSRSQIPAVREASQKRIANEQDKRKRLAAGELGLDIYKMREQLKALGLEY
                     R"
     gene            complement(2271..4613)
                     /locus_tag="VNZ06_10595"
     CDS             complement(2271..4613)
                     /locus_tag="VNZ06_10595"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWW21244.1"
                     /translation="MSSMLSGRYKEETYLWDVSSVCRSRPESAPRLLALIDRYKRLGR
                     MPAAQHEKVKARIEQVMSVIKPVTRKSSDSITRLAPPPATPAANEPSAADASALSDEE
                     AIAEFEDDSALPDDYSEESVTREFRANAAPTRAVAPASAAVVREALPRDAIPTRVLPP
                     SAMLEVMALESASRETRVLSPICEPPSEPGPAATRLLSPRPAAPASAALSPTPLPSTP
                     PALVSAITPIGVGAVLHERYELKQMLGRGGIATVYKAVDRYRVNLGLEDCYIAVKIVQ
                     PHPARPGSVAALGREFHNAQLLSHPNVINVYNIDHAGDASFYTMELLDGERVSQVVKR
                     VGVLPRRQALAIIRDIGAAIAHAHSRGVVHADLKPHNVMITRIGQVRVLDFGSGVIRA
                     REPWISEMSPDDSYRQATPAYASCEQLEGWSADPRDDIYALSCLAYLLLTGKHPFNML
                     PSLDARARRLQPRRPPGMRGEAWRALRRGLSWTREQRTMALEDWLQQLGVGSAVESLP
                     PLPLLQTGQPYNAWTHRAAAAAVIVAGLGLAAFTAEHQDQFDWHPTLAKAQNRLSDAW
                     RDLQSLTDAPSAAPAVAPPVAVEALPLPPPPAASSRKGSARGPSTATQRSVRTTAVDP
                     SAAAPAAEEIALAAPAADHQAITASGAALASTGYAEDPANSATPPRLEFSAPSYVVGG
                     TEPAARIVIRRNGSTEGDLNFVWWTEEASAKADVDYASLGRRIERIPSGTDKVTVYVP
                     IMSTPTRHEITQFYVALGEPGSLHSNGPGTRSLVTIDHGT"
     gene            4874..5494
                     /locus_tag="VNZ06_10600"
     CDS             4874..5494
                     /locus_tag="VNZ06_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21245.1"
                     /translation="MMPAKSYSTIFSFVALTTLQLLLSNSAVAASGDACGKLPATGFD
                     RPPNLPRTGRYVNTEYGYSVAIPAGLNGYVSAEGPERGFGIVLSWSPRAFIRVDAAYD
                     AFFDITSQGVHRSDLTTIRLHDSVVDDQSATFSLAHKQGGRYVTRLRCGTDPQIFIHD
                     QVIVMVNREIYRLELQTVPQRYDDDVKVINAMLQSWQWQRPAARSQ"
     gene            complement(5439..7274)
                     /locus_tag="VNZ06_10605"
     CDS             complement(5439..7274)
                     /locus_tag="VNZ06_10605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW21246.1"
                     /translation="MQGDDAAAQSTTALTPDHEMLVEMCGLVARVLQRMPWIESVLAI
                     SIGIFVYPFVPAPVFIGWAGLTIGVETMRAHYARGVLRRGHDIDPQRTHRIFVIHAGL
                     AGLAVGLSAVLFLPRLPILDQALLGIIVMIMPAAGVAVSQSSRYMAIAYSLGIVVPAV
                     GTWMALHPQQAAPTGILTTLYCALIFIVAAEGDQLLLRSIMIRHERDRLVRDLEQRNR
                     DVRAAMTQAEQSALARARVLAAASHDLRQPLHALSVYSAVLAANPPPDAMREVGSNID
                     QIVRSLGSLLHGLLDLSRLSSGHYTIEREPTSLDQLLRSVCAEFERPASEKGLTLNQC
                     LSPVSVVGDPVALGRIARNLLDNAIKYTERGSITVAASLDPSTSPPSAVMTVADTGKG
                     IPSGELSRIFEEFYQLDNPGRDRSKGVGLGLAIVQRLCELSGASISVESEVGVGTTFT
                     IRLSQLVESHGPLRKDEPTRERVVSLSGRKIYVVDDEIDIQKSMTQLLKLWGADARSA
                     ASPEAAVQLFETHGTPDLLITDLRLGAEENGAELALRLMRRFAHFPALITTGEISPEA
                     LKFTTDHGLGLLHKPITEEELRRAINQLIESAPPAAAIATTAASH"
     gene            complement(7278..7904)
                     /locus_tag="VNZ06_10610"
     CDS             complement(7278..7904)
                     /locus_tag="VNZ06_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWW21247.1"
                     /translation="MKVLLVDDHAMFRAGLRMLMTTICPGAAALEAATIAEALDLLAA
                     HPDAQLCLLDLSFKNEGGLAGLSRIKEAAPETAVVVLSGAEDTTTIHACLDAGAMSFI
                     PKSLGPDVLTKALQQVLQGKVYLPEQLIRAAIDMPPRPALTSRQLQVLQCLSRGLPTK
                     LIAREMSLSEHTVKEHIALVFQALSARNRTEAVIKGSQWRLLERPLAL"
     gene            8144..8557
                     /locus_tag="VNZ06_10615"
     CDS             8144..8557
                     /locus_tag="VNZ06_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21248.1"
                     /translation="MKPEPRLKSLGGVLSWFRRRTQVSTSERVNTRVVTNIRVTNPWH
                     AVAVSGGPSCCRASFLARSQRYLSTEAPPLPLAGCTQPKSCLCKYKHFSDRRAGPRRT
                     TDSNLYKNALSPHRIPRLTFQDRRGSKGRRADDGR"
     gene            8726..>10236
                     /locus_tag="VNZ06_10620"
     CDS             8726..>10236
                     /locus_tag="VNZ06_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21249.1"
                     /translation="MKFQIRDFLVALSALTLGLTHAVSTHADPAPFDLAGPDLTVTVT
                     RDGKTLPLGEVPNLAVGDKVYIHADLPATQSARYLLIAGLLRGSTNPPPSEWFFKCET
                     WTKPCAQAGLTLTVPADAQQVLLFLAPETGGDFRTLVNTVRGRPGAFVRASQDLNQAT
                     LDRSRLDAYLASIHQLDTSDPGRLKEVAPLLARSLAIKVDDKCLDRLPELQAPCLMQG
                     NTALILNDGHSTSIVEALTSGPAADLAMEASYTPQLQYGFYSPYIASVLDIARIFDSF
                     HIAQYQYIPALGTHRGERLTLSLNTPPSFHNPKSVIVIALPAVEAAQRPPLHAVDPKE
                     IFCARKSKLVLPVEGAPLVFSTAYAHDMTLSVAGSDGKNIDLPAKADAEQGGFVVDTT
                     VLGSAALGDSVHASLHGGWGFDDYRGPGFQLVNAHAQTWELASKDQATLIVGREDTVH
                     LQAGEVSCVDGLMIRDAAGKELKPEWKALKPTEVELKLPLQEVRPGSLTLLVS"
BASE COUNT         1879 a         3200 c         3266 g         1891 t
ORIGIN      
        1 atttcttccg ccgcacgaac atcaacgcgc tcgggcagcc acgaagctgg accgatcatc
       61 tgttgtggca ccacgcctgt cacacggtgg acctgttcca ataccagacc gaccagaggg
      121 tgtcggagtg ttacgcgcta caaggacccg cacatccgac actgggaatc gcgatggaca
      181 tgagcatcgg cctgaaagtt ccgggaggcg cgctctgtac gctgtcgctg tcgttcaaca
      241 acgacggtcc gctggggaca ttcttccgat acatctgcga taacgggact tatatctgcc
      301 gctatgacga tctgttcgat ggcaagaaca atcagatcga cgtgtcgagg gtggatgtgt
      361 cgatgaacgg cattgagctg caggaccgcg agttctttgc ggcgattgca cagcagcgcg
      421 agcccaatgc gagcgtcgcg cagtgcctga cggcgatggc gacgcttgac cggctcgagc
      481 gtatgctgac ggccagtgcc gcctcgggcg cacgatccgc atgatcatcg attgccacgg
      541 acattacacg acggcgcccg gggagctgca ggcgtttcgc gacgcacaga tcgaggcgtt
      601 caagcaagcc ggcgatgcct cctcggtaca ggcgccgcgc atcggtgacg atcagttgcg
      661 cgaaagcctg gaaaaggcgc agctcaaact gcaacgcgaa cgcggcaccg atctgacgat
      721 attctcgccg cgcgcatcgg ccatggcgca ccacatcggc aacgaggctg tgagcgcgca
      781 gtggacgcga atctgcaatg acctgatcgc gcgtgtctgc gggctctatc ctgaaaactt
      841 cgttggcgta tgccaattgc cgcagtcacc cggcgtgccg ccggctcatt gcgctgcgga
      901 actcgagcgt tgtgtgcgcg atctcgggtt cattggctgc aatctcaatc ctgatccttc
      961 aggaggacac tggaactcgc cgccgctgac cgacaggcat tggtatcccc tgtacgaaaa
     1021 gatggttggg ctcgacgtgc cggcgatggt gcacgtcagc gcttcctgca atcccaactt
     1081 tcacgccacc ggcgcgcact atttgaacgc ggacaccacc gcgttcatgc aatttctgac
     1141 ctcggacctg ttcaaggatt ttccgacgct acggttcatc atcccgcacg ggggtggcgc
     1201 cgtccccttt cactgggggc gctatcgggg cctggcgcag gatctgaaac ggccgccact
     1261 cgaagaactg ttgctcaaga acgtgtattt cgatacctgt gtgtatcacc tgcccggtat
     1321 cgagctgctg ttcaaggtcg tgccggttga gaacatcatt ttcggatcag agatggtggg
     1381 cgccgtgcgc ggcgtggatc cgcgctcagg tcattactac gacgataccc gccgctatgt
     1441 ggacgcgttg gacttgagcg cggcggaccg tcagcgagtc tatgccggca atgctcgacg
     1501 cgtattcccg cggttggatg ctcggctcaa agccgctggc cactagcgat attcgaggcc
     1561 gagggccttg agctgctcac gcatcttgta tatgtcgagg cccagctctc cggcggcaag
     1621 gcgtttgcgc ttgtcctgtt cgttcgcgat acgcttttgc gatgcttcgc ggaccgccgg
     1681 tatctggctg cgactgacca cgaccacgcc gtcatcgtcg gcgacgattg catcaccggg
     1741 ttccacccgg gcgccggcgc agaccacggg aacgttgacc gcgccgaggc tggctttgac
     1801 ggtgccctgg gcaaataccg ctttgctcca gaccggaaat ttcatgtcgg tcagggtcgc
     1861 gatatcgcgc acacccgcgt caatgatcaa tcctcttacc ccgtgcgcga cgcatgaggt
     1921 cgcgagcaga tcgccaaagt agccatcctc gcacgcagag ctgggggcca cgaccagtac
     1981 atcgcctggc cggcacactt ccatcgccac atgcagcatc cagttatccc cgggaggcac
     2041 cagcaccgtg accgcactgc cacagatgct gaccgggcga tagatcggcc gcaggtagga
     2101 agcgagcaac ccgatgcgac cctgtgcttc gtgcacggtg gcgacgccac attcacccag
     2161 tgcactgagc tcggcagcgg ctgctcgtgc gatttcgcgc acaacgacat gcttgctgat
     2221 ggcggctact ccctggggac gcgttgctgg cgtcgattat agcgatcgaa tcaggtgccg
     2281 tgatcgatgg tcaccagcga gcgcgtaccc ggtccgttgc tgtgcagtga gcccggctcg
     2341 cccagcgcga cgtagaactg cgtgatctca tggcgggtgg gcgtggacat gatcggcacg
     2401 tagaccgtga ctttgtcggt gccgctggga atgcgctcga tacgccggcc aagtgaggcg
     2461 tagtcaacgt cggctttggc ggaggcctct tcggtccacc agacgaaatt gaggtcgccc
     2521 tcggtgctgc cgttgcggcg gatcacgatc cgcgccgccg gctcggtgcc gccgaccaca
     2581 taactgggcg ccgaaaactc cagccgcggc ggggtggcgg aattcgccgg gtcctccgcg
     2641 tagcccgtgg acgcgagcgc agctccggaa gcggtgatcg cctgatgatc cgcggccggt
     2701 gccgccaacg cgatttcttc ggccgcaggc gcagcggcac tcggatccac agctgtcgtg
     2761 cgcacgctgc gctgcgttgc tgtcgagggg ccgcgcgcac tgcccttgcg agaggaggcc
     2821 gccggtggcg gaggcaacgg cagagcttcg actgcgacgg gcggcgcgac ggcgggagcc
     2881 gcgctcggtg catcggtcaa tgattgcagg tcgcgccagg catcgctcag gcggttctga
     2941 gccttggcca gtgtcggatg ccagtcgaac tggtcctgat gctcggctgt gaaggcggcc
     3001 aacccgagcc cggcgacgat cacggcggcg gccgccgcgc gatgcgtcca ggcgttgtag
     3061 ggctggccgg tctgtagcag cggtagcggt ggcagggatt ccacggcaga gccgacaccc
     3121 agctgctgca gccaatcctc gagcgccatc gtgcgctgct cgcgcgtcca cgataggccg
     3181 cgacgcagcg cccgccaggc ctcaccacgc atgcccggcg ggcgacgcgg ctgtaggcgg
     3241 cgtgccctgg catcgagtga gggcagcatg ttgaacggat gcttgcccgt gagcagcagg
     3301 tacgcgaggc aggacaatgc atagatatcg tcgcgcggat cagcgctcca gccttcgagc
     3361 tgctcgcagc tcgcatacgc cggggttgcc tgacggtagc tgtcgtcggg cgacatctcc
     3421 gaaatccacg gttcgcgggc gcggatcact ccactgccga agtcgagcac gcgaacctgg
     3481 ccgatgcgcg tgatcatcac gttgtgcggt ttcaggtccg cgtgtacgac accgcgcgag
     3541 tgcgcatgcg caatggccgc accgatgtcg cgaatgatcg ccagcgcctg gcgtcgcggc
     3601 agcacgccga cgcgcttgac aacctggctg acgcgttcgc cgtccaacag ctccatcgta
     3661 taaaacgacg cgtcaccggc gtgatcgatg ttgtagacat tgatgacgtt cggatgggac
     3721 agcagctgcg cattgtgaaa ctcgcgtccc aacgccgcca cgcttcccgg gcgtgctgga
     3781 tgaggctgaa cgatcttgac ggcaatgtaa cagtcctcga gaccgagatt gacgcggtat
     3841 cgatccactg ccttgtagac cgtggcgatg cctccgcggc ctaacatttg cttgagctcg
     3901 tagcgctcgt gcagcacggc accgacgcca atcggcgtga tcgcagaaac cagtgccggc
     3961 ggtgtggacg gcagcggtgt gggcgagagt gccgccgacg cgggcgctgc gggccgaggc
     4021 gatagcaacc gcgtcgcggc cggtccgggc tcgctcgggg gctcgcagat cggcgacagc
     4081 acgcgcgttt cacgtgaggc actttccaat gccatcacct ccagcattgc tgagggcggc
     4141 agaacacgcg tcggtatcgc gtcgcgcggc agcgcctcgc gtaccaccgc ggcgctcgcc
     4201 ggggcaacgg cccgggtcgg tgcggcgttg gcacggaact cgcgcgtgac cgactcttcg
     4261 gagtaatcgt ccggcaacgc cgaatcgtcc tcgaattcgg caatcgcctc ttcatcggac
     4321 agcgcgctcg cgtccgcagc gctgggctca ttggccgccg gtgtcgcagg tgggggcgcc
     4381 aaccgcgtga tgctgtcgga agacttgcgc gtcaccggct tgatgaccga catcacctgc
     4441 tcgatgcgcg ctttgacctt ctcgtgctga gcggcgggca ttcgtccgag tcgcttgtag
     4501 cgatcgatca gcgcgagcag tcgcggcgca gattccgggc gcgaacggca cacggaagag
     4561 acatcccaga gataggtctc ttctttgtag cggccgctca gcatcgagct catgagctct
     4621 cgcaatacgt cgtcgccgtc aaactgcttg cgtgtggtgg atccggacat acagttgtgg
     4681 acaataacgt cgagcggcaa tgcctataat tgcgtcgagg tgcacgaccc ctgtcgggag
     4741 catcctacct ccggttggac cactcgggca gtcctttgcc aaaaaagctt gcttgttgcg
     4801 ttccggttaa acgaggtggc gagatcgagg ttgacaacgg ctcaaagcgc gaggaaatga
     4861 cgaataattt caaatgatgc cggcgaagtc ctacagcaca atcttctcgt tcgtggcact
     4921 cacaacactg cagctactac tgtcaaattc ggccgtcgct gccagcggag atgcctgcgg
     4981 gaaacttccg gcgaccggat ttgatcggcc gcccaatctg ccgcgcaccg gtcgctacgt
     5041 gaataccgaa tacggctact cggtcgcaat accggcagga ctcaacggat acgtcagcgc
     5101 tgaaggacct gaaagaggat ttggcatcgt gctgtcgtgg agtccgcgcg catttattcg
     5161 cgtggacgcc gcatacgatg cgttcttcga catcacctcc cagggtgttc atcgcagcga
     5221 tctgacaacg atccgcctgc atgacagcgt cgttgacgat cagtccgcga cgttcagtct
     5281 ggcgcacaag caaggcgggc ggtacgtgac gcgcctgcgc tgcggcactg atccgcaaat
     5341 ctttattcac gatcaggtca tcgtgatggt gaatcgcgaa atttatcggc tggagctgca
     5401 gaccgtaccg cagcgctacg atgatgatgt caaagtaatc aatgcgatgc tgcagtcgtg
     5461 gcaatggcag cggccggcgg cgcgctctca atgagctgat tgatggcgcg gcgcaactct
     5521 tcctcggtga tcggtttgtg cagcaacccc aggccgtgat cggtggtgaa cttcagtgcc
     5581 tcgggcgaga tctcaccggt ggtgatcagc gccggaaagt gagcgaagcg acgcatgaga
     5641 cgcagcgcca gttccgcgcc gttctcctcg gctccgagac gcaggtccgt gatcagcaga
     5701 tccggcgtgc cgtgcgtttc gaacaattgc actgcggcct ccggggaggc cgcgctccga
     5761 gcgtccgcgc cccacagctt caacaactgc gtcatgcttt tctgaatgtc gatttcatca
     5821 tcgaccacat agatcttccg tccggacagt gacacaaccc gctctcgggt cggttcgtcc
     5881 ttgcgcaacg ggccgtgcga ctcgaccagc tgcgacagac ggatcgtaaa ggtcgtcccg
     5941 accccgactt cggactccac ggaaatgctc gcgccgctga gttcacacag tcgctggacg
     6001 atcgccaggc caagtccgac gcctttgctg cggtcacggc ccggattgtc gagctgatag
     6061 aactcctcga agatgcggct gagttcgccg ctcggaatgc ccttgccggt atcggcgacg
     6121 gtcatgaccg cgctgggtgg cgacgtgctc ggatcgagcg aggcggcgac ggtaatgctg
     6181 ccccgctcgg tgtatttgat cgcgttgtcg agcaaattgc gtgcgatgcg gcccagggcg
     6241 accggatcgc caacgacact caccgggctt aggcactgat tgagcgtcag acctttctcg
     6301 gaggccggac gctcgaactc ggcgcagaca ctgcgcagca gctgatcgag cgaagtcggc
     6361 tcgcgctcga tggtgtagtg cccgctggac agacgcgaca ggtcgagcag cccatgcagc
     6421 aggctgccca gcgagcgcac gatttgatcg atgttcgagc cgacctcgcg catcgcgtcc
     6481 gggggcggat tggccgccag caccgcgcta tacaccgaca aggcgtgcag tggctggcgc
     6541 aggtcgtgac tggctgcggc cagcacgcgc gcacgcgcca gggcggactg ctccgcctga
     6601 gtcattgcgg cgcgaacatc gcgattgcgt tgctccagat cgcgcaccag ccggtcgcgc
     6661 tcgtggcgga tcatgatcga gcgaagcaat agctgatcgc cttcggcggc gacgatgaag
     6721 atcagcgcgc aatacagcgt cgtcaggatg ccggtcggtg ccgcctgttg tgggtgcagc
     6781 gccatccagg tcccgacggc aggcacgacg atacccagag agtaggcgat cgccatgtaa
     6841 cgcgaggatt gagaaacggc gacccctgcc gccggcatga tcatcacgat gattcccagc
     6901 agcgcctgat ccaggattgg caggcgtggc aggaacagca ccgcgctcag tccgaccgca
     6961 aggcccgcca gcccggcgtg gatcacgaat atgcgatgag tgcgctgcgg atcgatgtca
     7021 tggccgcgtc ggagcacgcc acgggcgtaa tgcgcccgca tggtttcgac accgattgtc
     7081 agaccggccc agccaatgaa cacgggcgca ggcacgaagg ggtagacgaa aattccgatg
     7141 ctgatggcca gcaccgactc gatccagggc attctttgta ggacgcgcgc gaccagcccg
     7201 cacatctcga ccagcatttc atggtcgggc gtgagcgcag ttgtggactg tgctgccgcg
     7261 tcgtcacctt gcatgcatta cagggccaat ggtcgctcga gcagtcgcca ttggctgcct
     7321 ttgatcacgg cctcagttcg gttgcgggcg ctcagagcct ggaacaccag ggcgatatgc
     7381 tctttgacgg tgtgctcgga caggctcatc tcgcgcgcga tcagtttggt gggcagacct
     7441 cggctcaggc actgcagtac ctgcagctgg cgggaggtca gtgccggccg gggcggcatg
     7501 tcgatggcgg ctcgaatcag ctgttcgggc aagtacactt tgccctgcag tacctgctgc
     7561 aacgccttgg tcagtacgtc gggccccagg ctcttgggga taaagctcat ggcgccggcg
     7621 tccagacacg cgtgaatcgt cgtggtgtcc tccgcgccag agagcaccac cacagcggtc
     7681 tccggggcgg cctccttgat gcgactcagc ccggcgaggc cgccttcatt cttgaatgac
     7741 agatccagaa gacacaactg tgcgtccggg tgcgccgcca gcagatcgag cgcttctgcg
     7801 atcgtcgcgg cttcgagcgc ggcagctccg gggcagatcg tggtcatcag catgcgcagg
     7861 ccggcgcgaa acatcgcatg atcgtccacc agcaagactt tcactgcccc tcctgtcgca
     7921 ctttgcggta ctggattgat tgtagcgagg aaccggcgcg gtcgcggccc cctcttacga
     7981 ggggtgttgg aaggcggaga ggatcggcac gctgggctga accctacggt agggatcgct
     8041 gtcacgaagg atttcctatt tggcaattga acaatcagcc atcgcagtgc actcttgtgc
     8101 cttgacagaa cgcaacgatg actgcccagg gagtatcttc accatgaagc cagaaccccg
     8161 cttgaagagc ttgggcggcg tcttgtcgtg gtttcgccga aggacccagg tcagcacgag
     8221 cgagcgggtc aatacccgag tggtcaccaa tattcgtgtc acgaacccgt ggcacgcggt
     8281 ggccgtcagt ggcgggccgt cgtgctgccg cgcctctttt ctggctcgca gccagcgcta
     8341 tctgtcgact gaggcaccgc cgcttcctct ggccggctgc acgcagccga aatcctgtct
     8401 gtgcaagtac aaacacttca gcgatcggcg tgccggccca cgccgcacga ccgacagtaa
     8461 cctgtacaag aacgcgctat cgccgcacag gattccgagg ctgacatttc aggatcggcg
     8521 cggttccaag ggacgccgcg ccgacgacgg ccgctgattg tagaattttc agcttgcgcg
     8581 ccgtcgcgca acccagaacc acggtgacgc cgtggcggtc actactcccc tggcgctagc
     8641 gctactgtac cgcgcgatcc tcatcgcgcc taaaagcgtg cggcggttca tccatatcat
     8701 cgcaatgagg ctctaagata ctgagatgaa atttcaaatt cgcgattttc tggtggcgct
     8761 gagcgccctg acgctggggc tgacgcatgc cgtgagcact cacgccgacc cggcgccgtt
     8821 cgatctggcc ggtcccgacc tgactgttac ggtgacccgg gacggcaaaa cgttgccact
     8881 cggggaagtc cccaatctgg cggtggggga caaggtctac attcatgcgg atctgcccgc
     8941 cacccagtcg gcgcgctacc tgctgatcgc gggtttgctt cgaggctcca ccaatccgcc
     9001 gccgagcgag tggtttttca agtgcgagac ctggaccaag ccgtgcgccc aggcgggtct
     9061 gacactgacc gtgccagccg acgcgcagca ggtgctgttg ttcctggcgc ccgaaacagg
     9121 cggagacttc cggacgctgg tcaacacggt acgcggtcgg ccgggcgcct ttgtgcgtgc
     9181 ttcccaggat ctgaatcagg ccacgctcga tcgttcgcgc ctggacgcat acctcgcgtc
     9241 gatccatcag ctcgatacca gcgacccggg ccggctcaag gaggtggcac cgctgctcgc
     9301 gcgcagcctg gcgatcaagg tggatgacaa atgtctggat cgcttgccgg aactgcaggc
     9361 cccgtgcctg atgcagggca acaccgcgct catcctgaac gacggacaca gcacctcgat
     9421 cgtcgaagca ctcacctcag gccccgcggc ggatctggcg atggaagcca gctacacgcc
     9481 gcaacttcag tacggttttt acagcccgta catcgcttcg gtgctggaca ttgcgcgcat
     9541 cttcgattcc tttcacatcg cgcaatacca gtacattccg gcgctcggta cacaccgcgg
     9601 cgagcggctc acgctgtcgt tgaacacgcc gccgtcgttt cacaacccga agtcggtgat
     9661 cgtgattgcg ctgccggcag tcgaggccgc ccagcgtccg ccgctgcacg cggtggaccc
     9721 gaaagagatt ttctgcgccc gcaagtcgaa actggtgtta cccgtggagg gtgcaccgct
     9781 ggtgttctcg accgcgtacg cgcatgacat gactctgagc gtggcgggct cagacggcaa
     9841 gaacatcgat ctgccggcca aggcggacgc ggaacagggc ggcttcgtgg tcgatacgac
     9901 cgtgctcggc agcgcggcgc tgggtgacag cgtgcatgcc tcgctgcacg ggggctgggg
     9961 cttcgatgac taccggggcc cgggctttca gctggtcaac gcgcacgcgc agacatggga
    10021 actcgccagt aaggatcagg cgaccctgat cgtcgggcgc gaagacacgg ttcatctgca
    10081 ggccggcgag gtgagctgcg tcgacggact gatgatccgt gacgcggctg gcaaggaact
    10141 caagccggaa tggaaagccc tcaagccgac ggaggtcgag ctgaaactgc ctctgcagga
    10201 agtcagaccc ggttcgctca ccttgctggt gtcgca
//
LOCUS       DATMRM010000353         9235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3917380, whole genome shotgun sequence.
ACCESSION   DATMRM010000353 DATMRM010000000
VERSION     DATMRM010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9235
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3917380"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..653
                     /gene="pnuC"
                     /locus_tag="VNZ06_10625"
     CDS             54..653
                     /gene="pnuC"
                     /locus_tag="VNZ06_10625"
                     /inference="COORDINATES: protein motif:HMM:NF016837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HWW21250.1"
                     /translation="MIRDLLRALALTGPLELAAVGLGIVYVLLILKRNRLGWIAGGIS
                     SAIYVYLAASARLPMQSTLQAYYVVMAFYGWYSWTSAQQRPDGARIVRWPARHHLMAL
                     ACVAVLSLVSAQFLARETHAAWPYLDSFTTWVSLLATWMVARMKLENWLYWIIADAIT
                     AFLFAAQAHQASAALFVIYIVIAIIGFREWLQNYRLQLA"
     gene            650..1456
                     /locus_tag="VNZ06_10630"
     CDS             650..1456
                     /locus_tag="VNZ06_10630"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline/ethanolamine kinase family protein"
                     /protein_id="HWW21251.1"
                     /translation="MSALAPQVLAHVPGLEDGRPALRLEVLGGGSVNLVYRVDSPQGR
                     FVLRLDGPAWRRPGVDRARELSLHRLAAAAGIAPSVVAALPEQHGLLVTEFHEGRLWQ
                     AEDFGNPASLHRLGELLYRLHRVQAPQIAQFDPLQVGLGYVGLVRAEHAQAGRSAMRR
                     LEQACEPLRASERPAAVVHGDLSQGNLLEGERLWLLDWEYAQLTDPLMDLACLLAYYP
                     QAAPHAGELLAAAGFDARQHHQGLMARVEVYRMLTWLWHLARGESVAALS"
     gene            1531..1845
                     /locus_tag="VNZ06_10635"
     CDS             1531..1845
                     /locus_tag="VNZ06_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWW21252.1"
                     /translation="MPTVRVIDRDGVERELNAPTGSTLMEPLRDMDDGVAAICGGMCS
                     CATCHVYVDEAWVGKLPNPMSDETDMLGDLTSRKPNSRLSCQVVLNDALSGLKVTVAP
                     EE"
     gene            1849..2274
                     /locus_tag="VNZ06_10640"
     CDS             1849..2274
                     /locus_tag="VNZ06_10640"
                     /inference="COORDINATES: protein motif:HMM:NF018574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HWW21253.1"
                     /translation="MSGRWVYLILIVAAVLSIGRVIMTIRKARKLALHDDWDSQLVKN
                     LRAAGGNAFTPYQVDFFFTLPDEAACGSLRGLLEPEGFAVESRVATGEAATGYSVHAR
                     KQLRVTVSEMQEHSKRFRALAEQFGGYYDGWMTDPSRTA"
     gene            complement(2297..3049)
                     /gene="gpmA"
                     /locus_tag="VNZ06_10645"
     CDS             complement(2297..3049)
                     /gene="gpmA"
                     /locus_tag="VNZ06_10645"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF010713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HWW21254.1"
                     /translation="MPGKLVLVRHGQSIWNVENLFTGWTDVDLSEAGCQEAQQAGREL
                     LREGIVPQMVFTSVLKRAIRTQWLLLEVMDLLWLPVERTWRLNERHYGALQGLNKTQT
                     VERHGEAQVKIWRRSYDIPPPPLSADDPRHPRFDHRYADVDPNDLPATESLKDTLARV
                     LPYWSARIAPHLARGQHVLVVAHGNSLRALVKMLDGISEQAIVELNIPTGVPLLYEFD
                     ATMQARGSRYLGDPEAIRAAAEAVKQQTTQKL"
     gene            complement(3069..7856)
                     /locus_tag="VNZ06_10650"
     CDS             complement(3069..7856)
                     /locus_tag="VNZ06_10650"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HWW21255.1"
                     /translation="MSADGAPSRISRTRLTTSLLRAWRTSVPSEEWRTLDEAELRRAC
                     AAQLEFGTRRRRHQTLLRVTAAPPGGDGSYSVIELIVEDMPFLVDSLSMSLSQLSLSP
                     QVIVHPILRLRRDAAGHVRSLQPEIDVAPEESGGVRESWQYWRIDRISDEADCEHLRR
                     RLLSTLLDVRHAFNDWMRMRNSVLKLCNDISRNPPPLFADVIAESRALLQYMENHHFT
                     FLGYRESRIAPGSNGPVLAPVPHTALGLLRRRLPGARAPGAVTANIRRALRSRDLLII
                     TKANHRSTVHRAGYLDYIGVKRFNAAGRVIGEARILGLWATSAYRADLRHVPWLRHKM
                     KRVIERFRFAPNSHDAKRLVSILETLPRDELLQASVEDLIRCARAVLILQDRARVRLI
                     MRRDEFRRFWSCLVYLPRERCDPAAQARIETILRSACHGSEIDAALSITDSPLAQLHV
                     VVRVDALSNPRIDELKLEQAIAAALVSWRDRLRTALQARFGEAQAALLERRYATAFPD
                     SYRQDVDAALAVEDITDLQGVDAAVEKMQLRLYRPPQQRQQRLHLRILRREEALSVSE
                     VLPTFEHFGLRVIAERPYRLSWPDGSAAWVQDFELEHHELRRVEVASVAYELIAAFRA
                     VRAGELDDDGFHRLLIGAALTVRQVTVLRACCRYLLQTGIPFSQSYMERVLGAHAATA
                     RDLCELFEQRLNPRQVRGSAARAGVLEQRIRRAIEAVVSPDEDRILRAFLAVILATLR
                     TNYFRRDADGRPRAWLSMKFDPSKIPGLPVPRPAFEIFVHGPRVEGVHLRKGPIARGG
                     IRWSERPEDFRTEVLGLMKAQHVKNTVIVPVGAKGGFVARRLPPRAAREQQQREVIEC
                     YRSFIRGLLDITDNIVSARVLAPPDVHRLDGDDPYLVVAADKGTATFSDIANAISADY
                     GFWLGDAFASGGSSGYDHKKMGITARGAWECIKRHFRELNIDIQREAFTVAGIGDMSG
                     DVFGNGMLLSRQLRLVAAFNHQHVFLDPNPDAARSYRERARLFKLPRSGWNDYNQRLL
                     SRGGIIFERSVKSVTLSAQVRELLGLTQQRASPVEIIRAILCMRVDLIWNGGIGTYVK
                     ASDERNGEIGDRGNDAVRVDGRQVRARVIGEGGNLGLSQRGRIEYAAGGGKLNPDFID
                     NSAGVNTSDVEVNLKILLDAPGERALPRTVRDRLLSDATDEVAQLVLRNNYLQSEALS
                     LMERGAAEHLNEHRQMLRWLERYGELDRAVEFLPTDEELEERRRQGRGLTRPELALLF
                     AYGKIAMNRALTQTGSATDPYLARELERYFPQALRKRFADRIKRHRLRTQIIITTTTN
                     SIINRVGPTLLMQSGQQSGADAAAVARAYTIARDSADLRTLWAQIEALDGRVKTDDQY
                     DALLDTSRYLTHYTLWLLARRREYAEVGVSVTRLQPAIREFAHITPAALEGLDRERYL
                     ARSRRYIAQSLPTKLAENLAALEALRVAPDLVQLAARARAKARTVAGVHFGLSARLGL
                     DWLHGAIEQLPASGDWQRAAQTRLQAAALAAHLKISAGVLPHAGRGQRARAPGADPAL
                     ERWQQMLRDVRSLATPDFAALTVAVEALETLAGARHKTLVSLL"
     gene            complement(7853..9013)
                     /locus_tag="VNZ06_10655"
     CDS             complement(7853..9013)
                     /locus_tag="VNZ06_10655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWW21256.1"
                     /translation="MSVFKLPDLGEGLSEAEILRWHVKVGDHIEVDAPLLSVETAKAV
                     VEVPSPMSGRITRLHAAPGDRLEIGAPLVEFATDTPTPDSGTVVGHMPVASAEDVNSA
                     ALNAAHAALAARVRAVPAARALARSLGVELTGLTGTGRNGLITLDDVMAVGLPARTAG
                     SKMHPAAPRRSPDETGDVEVLRSLRRTMAQSMALSRDSVMECSVFDDADLHAWSARSD
                     YTGRVLRAITAGVGAEPVLNAWFDAQSQSRTLFEHIDVGIAVDTSDGLLVPVIRQVQK
                     RTPSQLREDLDRLKRAARDRTIGGEELRNFTFMLSNFGTMAGRYATPVVVPPAVAILG
                     TGRVRPDVVAVGDRIEIHLRMPLSLTFDHRVVTGGEAVRFLAAVIADLEQPE"
     gene            complement(9030..>9235)
                     /locus_tag="VNZ06_10660"
     CDS             complement(9030..>9235)
                     /locus_tag="VNZ06_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWW21257.1"
                     /translation="IVHEAPRTGGVGAEIAATVTEHALYDLQAPIRRVTGYDTIMPLY
                     RLEHDYIPSVARIVGGIRDTLAD"
BASE COUNT         1505 a         3046 c         3048 g         1636 t
ORIGIN      
        1 gagggaaatg acaaggcggt gttcgtgatg aaggttctcg gacccttcga ttcgtgatcc
       61 gggacctgct gcgggcgctg gcgctcaccg gtcccttgga gcttgctgcg gtggggctgg
      121 gtatcgtcta tgtgctgctc atcctcaaac gcaatcgcct cgggtggatt gccgggggga
      181 tcagttcggc gatctacgtg tatctggcag ccagtgcgcg cctgccgatg cagtccacgc
      241 tgcaggcgta ctacgtggtg atggcgttct atggctggta tagctggact tccgcgcagc
      301 agcggccgga cggtgctcgc atcgtgcgct ggccggcgcg ccatcatctg atggcgctcg
      361 cctgcgtcgc ggtgctgagc ctcgtgtccg cgcagtttct ggcacgcgaa acccatgccg
      421 cctggcccta tctggattcc ttcaccacct gggtcagtct gctggccacc tggatggtgg
      481 cgcggatgaa gctggagaac tggctctact ggatcattgc cgatgcgatc acggcgttcc
      541 tgtttgccgc gcaggctcac caggccagcg ctgcgctgtt cgtgatctac attgtcattg
      601 cgatcattgg cttccgcgaa tggttgcaga actaccgcct gcagctcgca tgagcgctct
      661 ggcgccgcag gtgctggcgc atgtgcccgg tctggaagac gggcggccgg cgctgcgcct
      721 ggaagtgttg gggggcggca gcgtcaatct cgtctatcgc gtagacagtc cacagggccg
      781 atttgtcctg cgcctggacg gtccggcctg gcgccgcccc ggggtggatc gcgcgcgtga
      841 gctgagcctg caccggctag cggccgccgc ggggattgcg cccagcgtcg ttgccgcgct
      901 tcccgagcaa cacggccttc tggtgactga atttcacgag ggacgcttgt ggcaggcgga
      961 agactttggc aatccggcgt cgttgcatcg actgggcgag ctcctgtacc gactgcaccg
     1021 ggtgcaggcg ccgcagattg ctcagttcga tcctctccag gtggggctgg gctacgtcgg
     1081 gctggtccgc gccgagcatg cgcaggccgg tcgcagcgca atgcggcggc tggagcaggc
     1141 ctgcgagccg ctacgcgcga gcgagcggcc ggcggccgtg gtgcatgggg atctgtccca
     1201 gggcaacctg ctggaaggtg agcgactgtg gctgctggac tgggaatacg cccaactgac
     1261 cgatccgctg atggatctcg cctgcctgct agcctactac ccgcaggccg cgccgcatgc
     1321 gggcgaattg ctggcggctg ccgggtttga cgcccgccag catcaccagg ggctgatggc
     1381 acgcgttgag gtctatcgta tgctcacctg gctgtggcac ctggcgcgtg gtgagagtgt
     1441 ggccgcgctg tcctaaagcc agctgcgagg gctggtaagc ggcaggcgtc gccggcaaac
     1501 tatcgcgccc caatcgctac tggagagacc atgccgactg tgcgagtgat cgaccgcgac
     1561 ggagtggaac gcgaattgaa cgcgcccact ggctccacac tgatggaacc cctgcgcgac
     1621 atggacgatg gggtggcggc gatctgcggc ggcatgtgct cctgcgccac gtgccacgtc
     1681 tatgtggatg aggcgtgggt cggaaagctc cccaatccga tgtccgatga gacagacatg
     1741 ttgggtgatc tgaccagccg caagcccaat tcacgcttgt cgtgccaggt tgtcctgaac
     1801 gatgcgctgt cgggtctgaa ggtcaccgtt gcgccggagg agtgatccgt gtcggggcgg
     1861 tgggtatatc tgattctgat tgtcgcggcc gtgctgtcga tcggccgggt catcatgacc
     1921 atcaggaagg ctcgcaagct ggccctgcat gacgactggg attcgcaatt ggtcaagaat
     1981 ctgcgcgccg ccggcggcaa cgccttcacg ccctaccagg tcgacttctt cttcacgctg
     2041 cctgacgagg cagcgtgcgg ctcactgcgc ggcctgctcg agccggaagg ctttgcggtg
     2101 gagtcacggg ttgcgacggg cgaggcggcc accggctatt cggtacacgc gcgcaagcag
     2161 ctgcgtgtga cggtgtccga aatgcaggaa cattccaagc gcttccgcgc gctggcggaa
     2221 caattcggcg gctactacga cggctggatg acggaccctt cccgcaccgc atagtgcgcg
     2281 acggtccgga tcggccctag agtttctgcg tggtctgctg tttgaccgct tccgcggccg
     2341 cgcgaatcgc ctccgggtcg cccagatagc ggctgccacg cgcttgcatc gtcgcatcga
     2401 attcgtacag cagaggcaca ccggtgggta tgttcagctc gacgatggcc tgctcggaga
     2461 tgccgtccag cattttgacc agggcccgca gactgttgcc gtgcgcaacc accaatacgt
     2521 gctggccgcg cgccaggtgc ggtgcaatgc gcgcgctcca gtagggcagt acgcgtgcca
     2581 gggtgtcttt cagcgattcg gtggccggca ggtcgtttgg atcgacgtcg gcgtagcggt
     2641 gatcaaagcg cggatgacgc ggatcgtcgg cgctcagcgg tggcggcggg atgtcgtagc
     2701 tgcggcgcca gatcttcacc tgggcttcac cgtgacgctc gaccgtctga gttttgttca
     2761 gaccctgtaa cgcgccgtag tgacgctcgt tcagccgcca ggtgcgctcg accggcagcc
     2821 acagcagatc catcacctcg agcagcagcc attgggtgcg gatcgcacgc ttcagcaccg
     2881 aggtaaaaac catctgcggc acgatgcctt cgcgcagcag ttcgcggcct gcctgctgcg
     2941 cttcctggca accggcctcc gacagatcga cgtcggtcca gccggtgaac aggttctcga
     3001 cgttccagat actctgaccg tggcgaacca gtacgagctt tccaggcacg gcttagctcc
     3061 tcaggggttt acaacaggct gacaagcgtc ttgtggcgag cgcccgccag cgtctccagc
     3121 gcctcgaccg ccaccgtcag tgcggcgaaa tccggcgtcg ccagtgagcg cacatcgcgc
     3181 agcatttgct gccagcgctc gagcgcagga tcggcgcccg gggcgcgcgc gcgctgtcct
     3241 cgccctgcgt gcggcagcac ccccgcgctg atcttcagat gtgcggcgag cgcggccgcc
     3301 tgcaaacgcg tctgtgcggc acgctgccag tcgccgctcg ccggcagctg ttcgatcgca
     3361 ccgtgcagcc agtccagtcc gagtcgggcg ctcaggccaa agtgcacgcc ggccacggtg
     3421 cgcgccttgg cgcgcgcgcg cgcggccagc tgcaccaggt ccggtgccac ccgcagcgct
     3481 tcgagcgccg ccaggttctc ggccagcttg gtgggcaggc tctgagcaat gtatcgccgg
     3541 ctgcgggcca ggtagcgctc gcgatccagc ccttcgagcg ctgcgggtgt gatgtgcgcg
     3601 aattcgcgga tcgcaggttg cagccgtgtc accgaaacac cgacctcggc gtactcacgg
     3661 cgacgcgcca gcagccagag cgtgtagtgc gtcagatagc ggctggtatc cagcagcgca
     3721 tcgtactgat cgtcggtctt gacgcgcccg tcgagggctt cgatctgcgc ccacagtgtg
     3781 cgcaggtccg cgctgtcgcg cgcgatggta taagcgcgcg ccaccgctgc ggcatcggca
     3841 ccgctctgct ggccgctttg catcagcagc gtcggaccca cgcggttgat gatgctgttg
     3901 gtggtggtgg tgatgatgat ctgggtgcgc agccgatgtc gcttgatgcg atcggcaaag
     3961 cgcttgcgca gcgcttgcgg gaaatagcgt tccagttcgc gcgccaggta cggatcggtg
     4021 gcactgccgg tctgcgtcag tgctcggttc atggcgatct tgccgtaggc gaacagcagt
     4081 gccagttccg gccgtgtcag tccgcgcccc tgccggcgcc gctcctccag ttcctcgtcg
     4141 gtcggcagga actccaccgc gcgatcgagt tcgccgtagc gctccagcca gcgcagcatc
     4201 tgtcgatgct cgttcagatg ctcggcagca ccacgctcca tgagactgag ggcttcgctt
     4261 tgcaggtagt tgttacgcag cacgagctgc gccacctcat cggtcgcatc ggacagcaag
     4321 cggtcgcgca ccgtgcgtgg caacgccctt tcccccggcg cgtccagcag gatcttcaga
     4381 ttcacctcca cgtccgaggt gttcacgccg gcggagttgt cgatgaagtc cggattgagc
     4441 ttaccgccgc cggctgcgta ctcgatgcga cctcgctgcg acagaccgag gttgccgcct
     4501 tcgccaatga cgcgcgcacg cacctgacgg ccatccacgc gcaccgcgtc gttgccgcga
     4561 tcgccgattt cgccgttgcg ctcatccgag gccttgacgt aggtgccgat accgccgttc
     4621 cagatcagat ccacccgcat gcacaggatc gcgcggatga tttccaccgg gctcgcgcgc
     4681 tgctgcgtca gtcccagcag ttcgcgcact tgtgctgaca gcgtgacgct cttgacgctg
     4741 cgctcgaaga taatgccgcc ccgggacagc aggcgctggt tgtagtcgtt ccagccggag
     4801 cgcggcagct tgaacaggcg tgcacgctcg cggtagctgc gggccgcatc cggattcgga
     4861 tccaggaaca catgctggtg attgaatgcc gccaccagcc tgagctggcg cgacagcagc
     4921 atgccattgc cgaacacatc gccggacata tcgccgatgc cggccaccgt gaaggcctca
     4981 cgctggatat cgatgttgag ctcgcggaag tgacgcttga tgcactccca ggcgccgcgc
     5041 gcggtaatgc ccattttctt gtggtcgtag ccgctggagc cgcccgaggc gaacgcatcg
     5101 ccgagccaga agccgtagtc ggccgagatc gcattggcga tgtcggagaa ggtcgctgtg
     5161 cctttgtccg ccgcgaccac cagatacgga tcgtcgccgt cgagccggtg cacgtcgggc
     5221 ggcgcgagca cgcgagcgct gacgatgttg tcggtgatat cgagcaggcc acggatgaaa
     5281 ctgcggtagc actcgatcac ttcgcgctgc tgctgttcac gtgccgcgcg cggcggcaga
     5341 cggcgtgcga cgaagccgcc tttggccccg accggcacga tgacggtgtt cttgacgtgc
     5401 tgcgctttca tcaggcccag cacttcagtg cggaaatcct ccggtcgctc ggaccagcgg
     5461 atgccaccac gggcgatcgg tcccttgcgc aaatgcacgc cctcgacgcg cggaccgtgc
     5521 acgaagatct cgaacgccgg acgcggcacc ggcaggccgg gaatcttgct cggatcgaac
     5581 ttcatcgaca gccatgcccg cggcctgccg tccgcgtcac gccggaaata gttggtgcgc
     5641 agcgttgcca gaatcaccgc cagaaatgcc cgcagaatgc ggtcttcgtc tggactgacc
     5701 accgcttcga tcgcacgccg gatgcgctgc tcgagcacgc cggcgcgggc cgccgagccc
     5761 cgcacctgac gcggattcag ccgctgctcg aacagttcgc acagatcacg cgcggtggcg
     5821 gcgtgcgcgc cgagcacccg ctccatgtag ctctggctga acggaatgcc cgtctgcagc
     5881 agatagcggc agcaggcccg cagcaccgtc acctgacgca ccgtgagcgc tgcgccgatc
     5941 agcagccggt gaaagccatc gtcgtcaagc tcgccggccc gcaccgcgcg aaatgcggcg
     6001 atcagctcat aggcaacgct ggccacttcc acgcgtcgca gctcgtggtg ttcgagctca
     6061 aagtcctgca cccacgcggc ggagccatcg ggccagctca ggcgatacgg ccgctcggcg
     6121 atgacccgaa ggccgaagtg ctcgaacgtc ggcagtacct ccgaaaccga cagcgcttcc
     6181 tccctgcgca ggatgcgcag atgcagacgc tgctggcgtt gctggggcgg ccgatacagc
     6241 cgcagctgca tcttttccac cgcggcatcc acaccctgca ggtcggtgat gtcctcgacc
     6301 gcgagcgcgg catcgacgtc ctgccggtag gagtcaggga aagcggtcgc atagcggcgc
     6361 tccagcaacg cggcctgcgc ctcgccaaag cgcgcctgca gggcggtacg cagccggtcg
     6421 cgccacgaca ccagtgcggc ggcgatcgcc tgctccagct tcagctcgtc gatccgcggg
     6481 ttgctgagcg cgtccacacg caccaccaca tgcagttgcg ccagcggtga gtcagtgatg
     6541 gacagcgccg catcgatctc gctgccatgg caggccgaac gcaggatcgt ttcgatgcgc
     6601 gcctgcgcgg ccggatcgca acgctcgcgc ggcagataga ccaggcacga ccagaagcgc
     6661 cggaattcat cgcgccgcat gatcaggcga acgcgcgccc gatcctgcag tatcagcacc
     6721 gcgcgcgcac agcggatcag atcctcgaca ctcgcctgca gcagttcatc gcgcggcagc
     6781 gtctcgagga tgctcaccag acgcttcgca tcgtggctgt tgggcgcgaa gcggaacctc
     6841 tcgatgacgc gcttcatctt gtgccgcagc cagggcacgt ggcgcaggtc ggcacggtag
     6901 gcgctggtgg cccacaggcc caggatgcgc gcttcaccga tcacacgtcc cgcggcgttg
     6961 aagcgtttca cgccgatata gtccagatag cccgcgcgat gcacggtcga gcgatggttg
     7021 gccttggtga tgatcagcag atcgcgcgag cgcaaggcgc gacgaatatt ggcggtcacc
     7081 gcgcccgggg cgcgcgcgcc gggcaatcga cggcgcagca accccagcgc ggtgtgcggc
     7141 accggcgcca gtaccggtcc gttcgacccc ggcgcgatgc ggctctcgcg atagcccagg
     7201 aacgtgaagt gatgattctc catgtactgc agcaacgcgc ggctctcggc gatcacatcg
     7261 gcgaacagcg gcggcggatt gcggctgatg tcgttgcaca atttcaatac cgaattgcgc
     7321 atgcgcatcc agtcgttgaa tgcgtgccgc acgtccagca gcgtcgacaa cagtctgcgg
     7381 cgcaggtgct cgcaatcggc ctcatcgctg atgcgatcga tgcgccagta ttgccaggac
     7441 tcgcgcacgc cgccgctttc ctccggcgct acatcgatct ccggttgcag ggacctgacg
     7501 tggccggccg catcgcggcg cagccgcagg atcggatgca cgatcacctg cggcgacagg
     7561 ctgagctgcg acagcgacat gctgagcgaa tccaccagga acggcatgtc ttcgacgatc
     7621 agctcgatga ccgagtagga gccatcgcca ccgggcggcg cggccgtgac gcgcagcagc
     7681 gtctgatggc gtcggcgccg tgtaccgaac tccagttgcg ccgcgcaggc gcgccgcagc
     7741 tcggcctcat cgagcgtgcg ccattcctcg ctcggcaccg aggtgcgcca cgcacgcagc
     7801 agcgacgtgg tcaatctggt gcggctgatg cgggacggtg cgccgtccgc gctcactccg
     7861 gttgctccag atcagcgatg actgccgcca gaaatctcac agcttcaccc cccgtgacga
     7921 cacggtggtc gaatgtcaat gataacggca tccgcaggtg aatctcgatt cggtccccaa
     7981 ccgcaaccac atccgggcgc acgcgcccgg ttcccaggat cgcaaccgcg ggcggcacga
     8041 ccaccggtgt cgcatagcga ccggccatgg tgccgaagtt cgacagcatg aaagtgaaat
     8101 tgcgcagttc ctcgccgccg atcgtgcgat cacgcgcggc gcgcttgagc cggtccagat
     8161 cctcgcgcag ttgcgacgga gtgcgcttct gcacctgcct gatgaccggc accagcagtc
     8221 cgtcgctggt atccacggcg atgccgacat cgatgtgctc gaacagcgtg cggctctggg
     8281 attgagcgtc gaaccacgcg ttcaacaccg gctcggcgcc tacgcctgcg gtgatcgccc
     8341 gcagcacgcg tccggtgtag tcgctgcgtg cgctccaggc atgcagatcg gcgtcgtcga
     8401 acaccgagca ttccatgacg ctatcgcgtg acagcgccat gctctgcgcc atcgtgcgtc
     8461 gcaggctgcg aagtacttcc acatctccgg tttcatcggg cgatcgacgc ggggcggccg
     8521 gatgcatctt gctgccggcg gtgcgcgctg gcagtccgac ggccatcacg tcatcgagcg
     8581 tgatcagtcc attgcgcccg gtgccggtca gaccggtgag ctccacaccg aggctgcgtg
     8641 cgagcgcacg ggcggccggc acggcgcgaa cgcgcgcggc gagggcggca tgtgccgcat
     8701 tcagcgccgc actgttgaca tcctctgcgc tcgccaccgg catgtggccg accacggttc
     8761 cggagtccgg ggtcggtgtg tcggttgcaa attcgaccaa cggggctccg atctccagtc
     8821 gatcgcccgg tgcggcgtgc agccgggtga tgcgaccgct catcggcgag ggcacttcga
     8881 ccaccgcctt ggcggtctcg accgaaagca gcggcgcatc cacttcaatg tgatcgccga
     8941 ccttgacgtg ccagcgcagg atctcggctt ctgacagacc ctcgcccaga tcgggtagtt
     9001 tgaagacgct cacctggtat cgcccttcgt tagtcagcga gcgtgtcacg gatgccaccg
     9061 acgatgcgcg ccacgctcgg aatgtaatcg tgctccagcc ggtacagcgg catgatcgtg
     9121 tcgtagccgg tcacacgccg aatcggtgcc tgcagatcgt acagcgcgtg ctcggtgacg
     9181 gtggccgcga tttcggcgcc gacaccgccc gtgcgggggg cctcatgcac gatca
//
LOCUS       DATMRM010000354         6230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_831800, whole genome shotgun sequence.
ACCESSION   DATMRM010000354 DATMRM010000000
VERSION     DATMRM010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831800"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..999
                     /locus_tag="VNZ06_10665"
     CDS             133..999
                     /locus_tag="VNZ06_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HWW21258.1"
                     /translation="MNASDETDTLQRMQALWRTVLNLQSVGPDDNIFDLGADSVAVVR
                     LVAAVAKEFEVSLAPRDIYASVTPRRVSSMLRVIVRARLRYSYMTRLPNGLAIRCPSA
                     AGARAQFDTVFRQRHYMRHGIVLPARAGVLDLGAGIGMFSMFAALEAPLASIVSVEAS
                     IPQFEMLKFNLVDVSDNAVALNADGRVSVCSLSDTLQAAYEHLRGNGLAAVDLLRVDL
                     NWAGAALLLGFKSNQWERIHQVVADVSDKDILGVCHTLESQGFRIRVDRSAPGAGAVS
                     VYGVRVSYAEHT"
     gene            1317..2237
                     /locus_tag="VNZ06_10670"
     CDS             1317..2237
                     /locus_tag="VNZ06_10670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HWW21259.1"
                     /translation="MDDNSEVAGKEPGVGVVRAPRISKLSAPSAPSASPTIISINPFK
                     CRVWALHDRLDDYVNEESCKAEIESFAAHGQLVPTLGRALRNDPDYDVEIIYGARRLF
                     VAQHLGVALKVELREMSDREAIVAMDIENRHRKDISAYERGRTYQRWLRAMHFASQDD
                     IARALNVSSSQVSRLLKLSQLPSVIVAAFASPLDIREGWGIELYRAWHEKGLQGLMAQ
                     RARAIAERVPRLSAAEVYERLLAPPGGGLVIRAQRRDEVVRSAEGALLFRIRHQRKVV
                     ALLVPAASIVPKNLDRVRRALSEILQDVSM"
     gene            complement(2297..3004)
                     /locus_tag="VNZ06_10675"
     CDS             complement(2297..3004)
                     /locus_tag="VNZ06_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWW21260.1"
                     /translation="MKRYRRGSHDIEVLQRVSVSIDQREFLALMGPSGSGKTTLLNLI
                     AGLDRPTGGQIVVNGSRLDRLSGDELAVWRARNIGFVFQFYNLLPNLTAQRNVEVPLL
                     LTKLSRAERRQRTRVALQAVGLTDRAGYRPSELSGGQQQRVAIARALVSDPLLLVCDE
                     PTGDLDRESAKGIMEVMQMLNRSHGKTILMVTHDPLAAVYASRVLHLDKGSLLEPKAN
                     DYRSARAGSLQVVSSSD"
     gene            complement(2940..3908)
                     /locus_tag="VNZ06_10680"
     CDS             complement(2940..3908)
                     /locus_tag="VNZ06_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWW21261.1"
                     /translation="MNTFARRVELPHCPELCLHAQQWGSEAPSLLLIHGFGDGRYIWN
                     HVLPDILHYGSVVTVDLRGHGDSDHGPTSAYATSTHAADVAVVLESFFARNVILVGHS
                     LGAQVAIHLATSHVQMVRAVVLVDGGPEMDTSMTAHVRRTFASQRWRYTSIDDYADVL
                     RERLPLAAHSLLQALASHALCRDDSGGYSLKCDWAMGKEQIPDNEELIWNLFRSIRCP
                     CLLVRGEGSAILSRAAAAKMAEANPVCCVQSVPLAGHAVFVDNPDGFLGTLDSFLPRI
                     IDQVKASRDARGYETNDLLGTGDQCREALSPRITRHRSASASQRFD"
     gene            3995..5917
                     /locus_tag="VNZ06_10685"
     CDS             3995..5917
                     /locus_tag="VNZ06_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HWW21262.1"
                     /translation="MRISSVRLDNSAEQAMVVRALHSPRSMPGELIQELFEQQVERTP
                     HAVALTCAGATLSFAELNELANQIAWHLRAQGVGADQAVGLCVGRSLEMVVGILGILK
                     AGGAYVPLEPAHPVQRLAFMLADCAPRVVLTHASARAVLETVTRGLHHQPMLLDLDTE
                     LWREQPNSNPPAESIGLSGRHLAYVIYTSGSTGQPKGVMVEHRNVISHWHALEQLYRH
                     PFDCRRIGVNAAFTFDVSVQQIVQLLSGCTLFLIPEALRRDAGKLLDFIERYQIEGID
                     CTPSQLRAWVAAGLLQRTASALRTVLVGGESLEAALWSRLRECQSITFYNVYGTTECT
                     VDSTAAHLRDASAASQIGRPLNNSGIYILDAERSSVAIGVVGEIYIAGAGVARGYLNR
                     PDLTAERFLPDPFSNDSQAFMYRSGDLGRWCADGSIEYLGRTDQQLKIRGHRVELGEI
                     EARLRQHDAVCDVAVLAREDSAADRRLVAYWTASDPQLSCDPEALREHMAGYLPDYMV
                     PAAFVRMPSFPLTPSGKLDRRALPAPQLEDHTARSYQAPQGEIEQTLASIWQELLNIE
                     RVGRFDNFFDLGGHSLLVILALERVRVRLGLALSLSSMFEHPTLASLSNFVVYQSCAA
                     ATASSNAGGAKGDQYD"
     gene            5910..>6230
                     /locus_tag="VNZ06_10690"
     CDS             5910..>6230
                     /locus_tag="VNZ06_10690"
                     /inference="COORDINATES: protein motif:HMM:NF036900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21263.1"
                     /translation="MTSAQALLSRLRSKGVELWLRGAELMYEAPAGALTGGEVDELCL
                     RKAELIHQLSTMTDLTAPEEITSDAERANLAPLSFSQLRLWFMAQMEGGDTAYHISLG
                     VRLGG"
BASE COUNT         1260 a         1788 c         1903 g         1279 t
ORIGIN      
        1 gttggtacgt cccgccccag agtgcaacct gcccgccatc tgtggtgttc gtgttccccg
       61 gttcgggtgg agtcctcttc gagcagtttg acgagctcgc tgctcgctaa ccctccaagc
      121 gtggtcgccg agatgaacgc atcggatgag accgacaccc ttcagcgtat gcaggctctc
      181 tggcgcaccg tactgaacct gcagagtgtg ggtccagacg acaacatatt tgaccttggc
      241 gcagattccg tagccgtggt ccgcctggtt gcagccgttg ccaaagagtt tgaggtgtca
      301 cttgctccac gagacattta cgcatccgtg acgccgcggc gggtgagttc gatgctgcgc
      361 gtcattgtgc gcgcaagact gcgctacagc tacatgacga ggctgccgaa tggacttgcg
      421 atccgctgcc cgtctgctgc gggcgctcgc gcgcaattcg acaccgtgtt ccgtcaaaga
      481 cactacatgc gtcatggcat cgtactcccc gcgcgcgcgg gagtgctaga tcttggcgct
      541 ggaatcggta tgttctcgat gttcgcggcg cttgaagccc ccctcgcgag catcgttagc
      601 gtggaagctt cgataccgca gttcgaaatg ctgaagttca accttgtgga cgtgagcgac
      661 aatgctgtcg cgctcaacgc cgacggcagg gtatcagtct gctcgctgtc ggacacactt
      721 caggcagctt atgagcacct gcgcggcaat gggcttgcgg ccgtggatct gctgcgagtc
      781 gatctgaact gggcaggagc agccctgctc ctcggcttca aatccaatca atgggagcgg
      841 atccaccagg tggtagccga tgtttccgac aaggatatcc taggagtttg ccacaccctt
      901 gaatcccagg gatttcgcat ccgcgttgat cgctcagcgc cgggagctgg tgccgtctca
      961 gtttatggcg tcagagtctc ctatgccgag cacacctgac gcaaacgacc taaaggtacc
     1021 cagcgttgag tgccacacga gcagggcgtg ttcgtatgat caggcttctt gggcggatcg
     1081 ctggatcggt gatggccttg aaaaaactgc catacgagaa acgtatagaa atggtgcagc
     1141 gactgcccta cttgtgtcgt ccaggtcgcc gtttctgcgg cgctgtcctt gagggtggcc
     1201 gacatcttaa attcatctgc ggacctctat tcctttcatc tggttgcgat gtagtctcca
     1261 gatgagtcat gcaacagtgt gactcatcga agttgcacgt cagtgtaggg gaaccgatgg
     1321 acgacaacag cgaagttgcg ggcaaggagc caggcgtcgg cgtagtgcgc gcgccaagaa
     1381 tttcgaagct ttcggcacca tcggcgccgt cggccagccc gacgattatc tcgatcaatc
     1441 cgttcaaatg cagagtttgg gcactccatg accgactcga tgactacgtc aatgaggagt
     1501 cgtgcaaggc cgagatagag agttttgccg ctcacggcca gttggttcca acactgggac
     1561 gagcgttgcg aaacgacccc gactacgacg tggaaattat ctacggggcc cgaagacttt
     1621 ttgtagcgca gcacctgggc gtagccctca aagtagagtt gcgtgagatg tccgatcggg
     1681 aggcgatcgt tgccatggac atcgagaacc gtcatcggaa agacatcagt gcatacgagc
     1741 gcggaaggac ctaccagcgc tggctgcgag cgatgcattt cgcgtcgcag gacgacatcg
     1801 ctcgcgccct caacgtctcg tcatctcagg tgtctcgcct gctgaaactc tctcagctgc
     1861 cctcggtcat cgtcgccgcg tttgcgagtc ccctggatat ccgtgaaggc tggggaatcg
     1921 agctataccg agcgtggcac gagaagggcc tgcagggact gatggcgcag agggcacgcg
     1981 ccatagccga gcgagtcccc cggctgagcg ccgctgaggt ctacgaaaga ctgctcgcac
     2041 caccgggagg tgggctggtg atacgggccc agcgtcgtga cgaagtggta cgcagtgccg
     2101 aaggtgcgct gctgttccgc attcgccatc aacgcaaggt ggtggccctg ctagtcccgg
     2161 cagcatcgat cgttccaaag aatctggaca gggtgcgccg tgcgttgagc gaaatcttgc
     2221 aggacgtaag tatgtaagcg attgaagatg tggacgatta tgcagattcg acagcgacaa
     2281 cggtgaactg attcactcaa tcgcttgatg agacgacttg aagcgatccg gcgcgggccg
     2341 atcggtaatc gttggccttc ggctcaagca ggctaccttt gtccagatgc agaactcgcg
     2401 aggcatagac ggccgcgagc gggtcatgag taaccatcaa tatcgtcttg ccgtggctgc
     2461 ggttcagcat ttgcatgact tccataatcc ctttggcact ctcgcggtcg aggtcgcccg
     2521 tcggctcgtc gcacacgagc aacaacgggt cggacaccag ggcacgcgca atcgctacac
     2581 gctgctgctg gccgccggac agttccgaag gtcggtatcc cgcacgatca gtcagtccga
     2641 ccgcctgaag agccacgcgg gtccgctgcc tacgttcagc gcggctgagc ttcgtcagaa
     2701 gcagcggcac ttcgacgttg cgttgcgcag tcaaattggg caacagattg taaaactgaa
     2761 aaacgaatcc gatattgcga gcacgccaga cagcgagctc gtcaccggac aggcgatcga
     2821 ggcggctccc gttgacgaca atctgacctc cagtcgggcg atcgagtcct gcgatgaggt
     2881 ttagcagcgt tgtcttgcct gagcctgagg gacccatcaa ggcaaggaat tcacgttggt
     2941 caatcgaaac gctgactcgc tgaagcactt cgatgtcgtg tgatccgcgg cgatagcgct
     3001 tcacgacatt ggtcgcctgt accaagcaaa tcattggttt catatcctcg cgcgtcacga
     3061 gaagccttga cctgatcgat aatccgaggc agaaaggaat cgagcgttcc cagaaagccg
     3121 tcgggattgt ctacaaatac cgcatgacca gcaagaggta cgctttgaac gcagcacacg
     3181 ggattggctt ctgccatctt tgcagcggcc gctctggata gaatcgccga accctcacca
     3241 cggacgagga ggcaggggca tctgatggat ctgaacaggt tccatatcag ttcttcgttg
     3301 tccgggatct gctcttttcc catcgcccag tcgcatttga gggaatagcc gccgctatcg
     3361 tctcggcaca gcgcgtgtga tgcgagtgcc tgcaacaacg agtgagccgc cagcggcagc
     3421 cgttcccgca gcacatcggc gtagtcatcg atggacgtgt atcgccagcg ctggcttgcg
     3481 aaggtcctgc gaacatgtgc ggtcatgctg gtgtccatct cagggccgcc gtcaaccagc
     3541 acaacggcac gaaccatctg cacatgcgag gttgcaagat gaatcgcaac ttgcgccccg
     3601 agcgaatgcc ccacaagaat gacgttccgt gcaaagaacg actccagtac gacggcgacg
     3661 tcagctgcat gcgtcgaagt ggcataagcc gatgtgggtc cgtggtcgga gtcaccatgc
     3721 ccgcgcagat ccactgtcac gacggatccg tagtgcagga tatcgggaag cacatgattc
     3781 cagatgtagc gtccatcccc gaatccgtgg atgagcagca acgaaggcgc ttcactgccc
     3841 cattgctgcg cgtgcaaaca cagctctggg cagtgaggca gttcaacccg cctggcgaat
     3901 gtgttcatgt gcgccaacag actcgaatgt acacgcgaag cctactatat tcgcgtgatc
     3961 tcacgcagca tcacttagag aaggagcgac caaggtgaga atcagcagcg tgcgcctcga
     4021 caactccgcg gagcaagcca tggttgttcg cgcgcttcat tcaccgcgct caatgccggg
     4081 tgagctgata caggagttgt ttgagcagca ggtggagcgc accccgcacg ccgtggcgct
     4141 gacctgcgcc ggcgcgacgt tgagttttgc ggagctcaat gagcttgcaa atcaaatagc
     4201 gtggcatctg agagcgcaag gcgttggcgc tgatcaggcg gtgggcctgt gcgtcggacg
     4261 ctcactggag atggtcgtgg ggatcctggg gatactgaag gcgggcggag catacgtgcc
     4321 gctcgagcca gctcatcccg tgcagcggct ggcatttatg cttgcagact gtgccccgag
     4381 ggtggtgctg acgcatgctt cggcgcgcgc cgtgctcgag acggtcacgc gtgggctgca
     4441 tcaccaacca atgttgctcg atctggacac cgagctgtgg cgcgagcaac ccaactcgaa
     4501 cccgccggcg gaaagcatcg gattgagcgg caggcacctc gcgtacgtga tctacacctc
     4561 aggctccacg ggtcagccca agggtgtcat ggtcgaacat cgcaatgtga tcagtcattg
     4621 gcacgcacta gagcagctgt accgtcatcc gtttgattgc cggcgtatcg gcgtcaatgc
     4681 ggcgttcaca tttgacgtgt cggtccagca aatcgtacag ctgctttcgg gatgtacgtt
     4741 gttcctcata cctgaagctt tacgccggga tgccggcaag ctgctcgact tcatcgagcg
     4801 ctaccagatc gagggcattg attgcacgcc gtcacaactc agagcctggg tggctgcagg
     4861 tttgctgcaa cgtactgcat cagcgttgcg cacggtgctg gtaggtggcg agtcacttga
     4921 agccgcactc tggagccgcc tgcgagagtg ccagtcgata acgttctata acgtctatgg
     4981 cactaccgag tgcacggtgg acagtacggc agcccatctg cgggatgcgt ccgccgcgtc
     5041 gcagatcggg cgtcccttga ataacagcgg catctacata ttggacgctg agcgcagttc
     5101 tgttgccatc ggcgtggtgg gggagattta tatcgcgggg gctggtgttg cacgcggcta
     5161 cctcaatcga cctgatctga ctgccgagcg cttcttgcct gatcccttca gcaacgattc
     5221 tcaggcattc atgtaccgga gcggtgattt ggggcgctgg tgcgccgatg gctccattga
     5281 gtatctaggc agaaccgatc agcagctgaa gatccgcggc caccgcgtcg agctcggcga
     5341 gatcgaggcg cgtttacgcc agcacgacgc ggtgtgcgat gttgctgtgc tcgcgcgcga
     5401 ggattcagcc gctgacaggc gactggtggc ctactggacc gctagcgacc cgcagctttc
     5461 gtgtgatcct gaggcgctgc gcgaacacat ggcggggtat ctgccggact atatggtgcc
     5521 tgcagcgttc gtacgcatgc cgtcctttcc gctcacgccc agcggtaagc tcgatcgccg
     5581 ggcgctgccg gcaccgcaac tcgaggacca tactgcgcgc tcgtatcagg cgccacaggg
     5641 agaaatcgag cagacactgg cctctatctg gcaggaactg ctcaacatcg agcgcgtcgg
     5701 ccgattcgac aacttcttcg acctcggcgg ccactccctg ctggtcatcc tggcgctaga
     5761 gcgagttcgc gtccggctcg gtcttgcact ctctctgtcg tccatgtttg aacacccgac
     5821 gcttgcatcg ctatccaact ttgtcgtgta ccaatcatgc gcagcggcaa cggcgagcag
     5881 caatgctggg ggcgcgaaag gggaccaata tgactagcgc gcaagcgttg ttgtcgcgcc
     5941 tgcgcagcaa gggagtggaa ctgtggctgc gaggcgcgga gctgatgtat gaagcgcccg
     6001 ctggcgcttt gactggcggc gaggtcgacg aactttgctt acgtaaggcg gaactgattc
     6061 atcagctatc aacgatgacc gatctgactg cgccggagga gatcacgtca gacgcggagc
     6121 gcgccaatct ggccccgctc tctttttcgc agttacggct atggtttatg gcgcagatgg
     6181 aaggcggaga cacggcgtat cacatatcgc tgggcgtaag gctcggtggg
//
LOCUS       DATMRM010000355         5087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3282107, whole genome shotgun sequence.
ACCESSION   DATMRM010000355 DATMRM010000000
VERSION     DATMRM010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3282107"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VNZ06_10695"
     CDS             complement(<1..577)
                     /locus_tag="VNZ06_10695"
                     /inference="COORDINATES: protein motif:HMM:NF025098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21264.1"
                     /translation="MLEFARWKYALIVVVLLIGTLFALPNVFQRDLALQVERKSHAPM
                     DEFARQSIEDLLHKQQITIKRDFIDDGRLVLAFDDVAAQLKARDTVESTMSEVYRSAL
                     AYASRAPAWMRNLGLKAMPLGLDLRGGLYLLYQVDLTGAVTQLLDSYEQSFRHALNEA
                     KLPFTDITTTTSSSASSTNPGGADGLLVTLPQ"
     gene            complement(596..931)
                     /gene="yajC"
                     /locus_tag="VNZ06_10700"
     CDS             complement(596..931)
                     /gene="yajC"
                     /locus_tag="VNZ06_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HWW21265.1"
                     /translation="MNLLVSDAYAQTPGGGFGGGGLGPNVIILVVFVAVFYFLLIRPQ
                     QKRMKDQQSMLSRLASGDEVVTTGGILGRIIEVNDTFITLEIADGVRIKVQKSQIAQL
                     MPKGTLKSA"
     gene            complement(969..2105)
                     /gene="tgt"
                     /locus_tag="VNZ06_10705"
     CDS             complement(969..2105)
                     /gene="tgt"
                     /locus_tag="VNZ06_10705"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012270584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HWW21266.1"
                     /translation="MRGALGFEVSARDGAARLGRFKLPHGYIDTPSFMPVGTYGTVKG
                     LTPEELEALGAQIVLGNTFHLMLRPGAQIVGLHRDGLHGFMGWERPILTDSGGFQVFS
                     LTGLRQIDEGGVSFHSPVDGERIRMSPEDSMDVQRVLRSDIAMAFDECTAYPVTQEHA
                     RESMRRSMRWAVRSYDHYYRDEPPGHLFGIVQGGMYEALRLESVEALSQRAFPGYAIG
                     GLAVGESEEERLRILECLGPHLPADRPRYLMGVGYPQDIVAAVARGVDLFDCVMPTRH
                     ARNGHLFTATGTINIRNAVHQRDTGPIDPSCSCSTCQRYSRAYLRHLDRCKEILGVRL
                     ATLHNVHFYLNLMREIRAAIAQGRFAQLAARVLAVPRRIGTPVA"
     gene            complement(2102..3133)
                     /gene="queA"
                     /locus_tag="VNZ06_10710"
     CDS             complement(2102..3133)
                     /gene="queA"
                     /locus_tag="VNZ06_10710"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HWW21267.1"
                     /translation="MSTSFQRSDFDYELPANLIAQQPLSQRSASRLLVLHGAQQVHQG
                     RQMSDLPQLLQSGDLLVFNDTRVLPARLLARKHTGGRVELLLERPLPQQRALVQIRDS
                     KSVRPGAVLETDGGTVRIRQRQQPFWEIELPGETVAFFQSYGQVPLPPYIDREPTAID
                     RERYQSLLARVPGAVAAPTASLHFDEVLLAALAARGIDRTTITLHVGAGTFQPLRSES
                     LTEHRMHSERYAVPEAAVAAIARARASGHRVIAVGTTVARALESAALNSGSTLASVQA
                     DTALFIHPGFHFQVIDALLTNFHLPGSTLLMLVAAFAGRDAVLAAYRHAVREQYRFFS
                     YGDAMLVWP"
     gene            complement(3165..3249)
                     /locus_tag="VNZ06_10715"
     tRNA            complement(3165..3249)
                     /locus_tag="VNZ06_10715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3213..3215),aa:Leu,seq:caa)
     gene            3287..3829
                     /locus_tag="VNZ06_10720"
     CDS             3287..3829
                     /locus_tag="VNZ06_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWW21268.1"
                     /translation="MKFCPACGQPIEMRIPPGDNLPRAVCAACGAIHYENPRMVVGCV
                     PEWEGRILLCQRAIQPRLGYWTTPAGFMENGETLQGAAARESLEEALAQVQIGSLLSI
                     THVLHARQVHINFRGRLLDGKFGVGTESLQVGLYRESEIPWDQIAFPSIEFALRSYFQ
                     DLHNGTESVHFNDINRPLRY"
     gene            3885..4208
                     /gene="fdxA"
                     /locus_tag="VNZ06_10725"
     CDS             3885..4208
                     /gene="fdxA"
                     /locus_tag="VNZ06_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016138692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HWW21269.1"
                     /translation="MTFVVTETCIKCKYMDCVEVCPVDCFHAGPNFLVIDPDECIDCT
                     LCEPECPVEAIFSEEELPSGQESFKQINAELAKKWPVITEKGTPPADAKEWEGKPDKK
                     NLLER"
     gene            complement(4227..5054)
                     /locus_tag="VNZ06_10730"
     CDS             complement(4227..5054)
                     /locus_tag="VNZ06_10730"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HWW21270.1"
                     /translation="MKPYAALVSLALFATVALSAPPASQQASANNSASPASPEPAAKP
                     PSAPPSGSAPLLAVSPETLRAVAKAAGEGVKPEDVRPTPLPGIFEMRRGADVLYVSQD
                     GKYVLVGDLYRVAGRENLTEVRRSELRRELIDQLPESKMVVFSPPNPKYTVTVFTDVD
                     CPYCRTLHSQIDEYNRLGVKVRYVFFPRSGPDTESWYKAEQVWCSTDRRAALTQAKLG
                     KPLDAKICANTPIAQEYELGRQIGLEGTPGIVLSDGTMIGGYMPPDKLLAQIKLQSP"
BASE COUNT          972 a         1617 c         1601 g          897 t
ORIGIN      
        1 cctggggcag cgtcaccagc agtccgtccg ctccgcccgg attggtgctc gaggcgctgg
       61 aggaggtcgt ggtcgtgata tcggtgaagg gcagcttcgc ttcgttcagg gcgtgacgga
      121 agctctgctc gtagctatcg agcaactggg tcaccgcacc ggtcagatcc acctgataga
      181 gaagatacag tccgccgcgc agatccagcc ccagcggcat ggccttgagt ccgaggttgc
      241 gcatccaggc cggcgcgcgt gaggcgtagg ccagggccga gcgatagacc tcggacatcg
      301 tcgactcgac cgtatcgcgc gccttgagct gcgcggccac gtcgtcaaac gccaggacca
      361 gtcggccgtc gtcgatgaag tcgcgcttga tggtgatctg ctgcttgtgc agcagatcct
      421 cgatcgactg ccgcgcgaac tcgtccatcg gcgcgtgact cttgcgctcg acctgcagtg
      481 ccaggtcgcg ctggaacacg ttgggcaagg cgaacagtgt tccgatcagc agcacgacta
      541 cgatcagtgc gtatttccag cgcgcgaatt ccagcatagg tcgaagctct tcggctcagg
      601 cgctcttgag ggtgcccttg ggcatcagct gtgcgatctg actcttctgt actttaatgc
      661 gcacgccatc ggcgatttcg agggtgatga acgtatcgtt gacttcgatt atgcgaccga
      721 ggataccgcc ggtggtcacg acttcatctc cactggccag ccgcgacagc atcgactgct
      781 gatccttcat gcgtttctgc tgcgggcgga tcaacaggaa gtaaaaaact gcgacaaaca
      841 ccaccagaat gatgacgttg gggcccaagc cgccgccgcc gaaaccgccc cctggggtct
      901 gggcgtatgc gtcgctgacc agtaaattca tgctcgcctc taagctaccg ccggcaaagg
      961 ggggcgcatt atgccacagg cgtcccgatc cgccggggca ccgccaagac ccgcgcggcc
     1021 agctgcgcga agcggccctg cgcaatggcc gcccggatct cgcgcatcag attcagatag
     1081 aaatgcacgt tgtgaagcgt cgccagacgc actcccagga tttccttaca gcgatccagg
     1141 tgtcgcaggt aggcgcgcga atagcgctgg caggtcgagc agctgcagct gggatcgatc
     1201 ggaccggtat cgcgttgatg cactgcattc cggatattga tggtcccggt ggcggtaaac
     1261 agatgcccgt tgcgggcatg acgggtcggc atcacgcaat caaacaggtc caccccgcgc
     1321 gcgaccgcgg ccacgatgtc ctgcggatag ccaacaccca tcagatagcg tggccgatcc
     1381 gccggcagat gcggacccag gcattccaga atccgcagtc gctcctcctc gctctctccc
     1441 accgccagtc cgccgatcgc gtacccggga aacgcccgct gtgacagggc ctcgaccgat
     1501 tcgagccgca atgcctcgta catgcctccc tgcacgatgc caaacagatg gcccggcggc
     1561 tcgtcgcggt aataatgatc atagcttcgc accgcccagc gcatcgaacg ccgcatggac
     1621 tcgcgcgcgt gctcctgcgt caccggatag gcggtgcatt catcgaacgc catcgcgatg
     1681 tccgagcgca gcacgcgctg cacgtccatc gagtcctcag gactcatgcg gatgcgctcg
     1741 ccgtccaccg gggagtgaaa gctgacaccg ccttcatcga tctgtcgcag cccggtcaga
     1801 ctgaagacct gaaagccgcc tgagtccgtg aggatcgggc gctcccagcc catgaagcca
     1861 tgcagaccgt cccgatgcag tccgacgatc tgcgctccgg ggcgcaacat cagatgaaaa
     1921 gtattgccga gaacgatctg cgcaccgagc gcttcgagct cctcgggagt cagccctttc
     1981 acggtgccgt aggtgccgac cggcatgaag ctcggcgtat cgatatatcc gtgcggcaac
     2041 ttgaaacgcc ccagtcgcgc agcgccatcc cgtgcactga cctcgaaacc cagcgcgccc
     2101 ctcacggcca caccagcatg gcatcgccgt agctgaagaa gcgatactgc tcgcgcacgg
     2161 catggcgata cgcggccagc accgcgtcgc ggccagcaaa tgccgccacc agcatcagca
     2221 gggtggatcc gggcagatga aaattggtca acaatgcatc gatcacttga aaatgaaatc
     2281 ctgggtggat gaacaacgcc gtgtccgcct gcacgcttgc cagcgtcgat cctgagttta
     2341 aggcggccga ttcgagtgcg cgcgccaccg tggtccccac cgcgatcact cgatgcccgg
     2401 acgcgcgggc gcgggcgatg gcggccacgg ccgcctccgg caccgcgtag cgctcgctat
     2461 gcatgcgatg ctcggtgagc gattcgctgc gcagcggctg gaatgtgccg gcgcctacat
     2521 gcagtgtgat tgtcgtgcgg tcgatcccgc gagcggccag agccgccagc agcacctcgt
     2581 cgaaatgcag gctcgcagtg ggcgcagcga ccgcaccggg tacccgggcc aacaggctct
     2641 ggtagcgctc acgatcgatc gcggtcggct cccgatcgat gtagggcggc aacggtacct
     2701 ggccgtagga ttggaaaaac gccacggttt ccccaggcaa ttcgatttcc cagaagggtt
     2761 gctggcgctg ccggatgcgc accgtgccac cgtcggtctc gagcaccgct cccggccgca
     2821 cggacttgct gtcgcgtatc tggaccaggg cgcgctgctg cggcagcggc cgctcgagca
     2881 gcagttcgac gcggccgccg gtgtgtttgc gcgcgagcag acgcgccggc aacactcgcg
     2941 tatcgttgaa caccagcaga tccccgcttt ggagcagctg cggcagatcg ctcatctgcc
     3001 ggccctgatg cacctgctgc gccccgtgca gcaccagcag gcggctggcc gagcgctgac
     3061 tcaggggctg ctgggcaatg agatttgctg gcagctcata gtcgaagtcg ctgcgttgaa
     3121 aggatgtgct caggtgcgtg ccggaagggt cggtggatgc aggatggtgg cctcgctcgg
     3181 agtcgaaccg agatggacaa gtccggtcga ttttgagtcg actgcgtcta ccagttccgc
     3241 cacgaggcca gcgtcgcgcg gtacaggtac gataccttaa acctatatga aattctgtcc
     3301 cgcctgcggc caaccgatcg agatgcgcat acccccgggc gacaacctgc cgcgcgcggt
     3361 gtgcgcggca tgcggcgcga ttcactatga gaacccgcgc atggtggttg gctgtgtacc
     3421 ggagtgggag ggacgcatcc tgctgtgcca gcgcgccatc cagccgcggc tcggttactg
     3481 gaccaccccg gccggattca tggagaacgg cgagacgctg caaggcgccg cggcgcgcga
     3541 gtcgctggag gaggcgctgg cccaggtgca gatcggctca ctgctatcga ttactcacgt
     3601 gctgcacgca cgacaggtcc acatcaactt tcgcgggcgg ctgctcgacg gcaagttcgg
     3661 agtgggtacc gaaagcctgc aggtaggctt gtacagggaa tccgagattc cgtgggatca
     3721 gatcgccttc ccgagcatcg aattcgcgct gcgcagctac ttccaggacc tgcataacgg
     3781 aacggaatca gtacatttca acgacatcaa ccggcccctg agatactgac tggccacgcc
     3841 ggcgatggca caatacgcgg ccgccgatct ggcccggagc ccaaatgacc tttgttgtta
     3901 ctgaaacctg catcaagtgc aagtacatgg actgtgtgga agtgtgcccg gtggattgtt
     3961 ttcatgcggg gccgaatttt ctggtgattg atcccgacga gtgcatcgat tgcacgttgt
     4021 gtgaaccgga atgcccggtc gaggccatct tctcggaaga agagctgccc tcaggtcagg
     4081 agagtttcaa acagatcaac gccgagctgg ccaagaagtg gccggtgatc accgagaaag
     4141 gaacgccgcc cgccgacgcg aaggaatggg aaggcaagcc cgacaagaag aacctgctgg
     4201 agcgctagcg gatttcccgg cgatcttcag ggcgactgca gtttgatctg cgcgagcagc
     4261 ttatccggcg gcatgtagcc gccgatcatc gtgccatctg agagcacgat tcccggcgtg
     4321 ccttccagac caatctgcct gccaagctcg tattcctggg cgatcggcgt attcgcgcag
     4381 atcttcgcat ccagcggctt accgagcttg gcctgagtca gcgcggcgcg ccgatcggtc
     4441 gagcaccaga cctgttccgc cttgtaccag gattccgtat ccggacccga tcgcggaaag
     4501 aacacgtagc gcaccttcac gccgaggcgg ttgtactcat cgatctggct atgcagggtc
     4561 cggcagtacg gacaatcaac atcggtgaat acggtgaccg tgtacttcgg attcggcggt
     4621 gagaacacca ccatcttcga ctcgggaagc tgatcaatca gctcgcggcg cagctccgag
     4681 cgacgcactt ctgtcaaatt ctcgcgcccg gcgactctgt agagatcgcc gaccagcaca
     4741 tatttcccgt cctggctgac gtacaggacg tccgctccgc ggcgcatctc aaagatgccc
     4801 ggcagcggcg tgggcctcac gtcctcgggt ttgacgccct cgcccgcggc tttggcgacc
     4861 gcgcgcagcg tctcaggaga taccgccagc agcggcgcac tgcccgaggg cggcgcggac
     4921 ggcggcttgg cagccggttc cggcgaggcc ggcgaggcgg aattgttggc cgaggcctgt
     4981 tgcgacgcgg ggggcgcgga aagcgccacc gtcgcgaaca gcgccagcga caccagcgcg
     5041 gcgtagggtt tcatgcgtca aggatagcgc gtaatgcgct acgcggc
//
LOCUS       DATMRM010000356         8863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_529881, whole genome shotgun sequence.
ACCESSION   DATMRM010000356 DATMRM010000000
VERSION     DATMRM010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8863
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529881"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1157
                     /locus_tag="VNZ06_10735"
     CDS             78..1157
                     /locus_tag="VNZ06_10735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWW21271.1"
                     /translation="MKIAIVSDAWRPQINGVVTTLGKTAEAAERLGHTVEVISADGSA
                     SIACPSYPEIRLAIRPGRLVRQRLRQFAPEMIHIATEGPLGLAARNHCVRSKLTFTTS
                     YHTQFPDYANARWGVPASWGYSYLRWFHKPAVRTLVGTETVRTNLHAWGFSHLVPWSR
                     GVDTTLFRPDPQARAMANARWPRPIMLYSGRVAVEKNLEAFLRLQLPGTKLIVGDGPA
                     RATLQSRYPDAVFLGYRFGAELAWHMAAADVFVFPSRTDTFGIVMLEAMACGVPVAAY
                     PVQGPLDVVRDGVTGVLDEDLASAVQRALRLDTKQCRAFAQLQSWERCTRQFIGHLAP
                     ACERPALQRLRAQDQGAAVAQLKKG"
     gene            1206..2888
                     /locus_tag="VNZ06_10740"
     CDS             1206..2888
                     /locus_tag="VNZ06_10740"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HWW21272.1"
                     /translation="MKHRTLLLSLSMGAACAVSAATAPAAGVDPARLLQHIKVLESDE
                     FEGRLPGTVGEQKSVAYITGQFKQLGLTAGNPNGTYLQDVPLEGIAGSPSMALTAGGR
                     AIPMEHGHDFVASTYRIVPQVLISNSPLVFVGYGVQAPEYNWDDYKGLDAHGKTLVML
                     INDPPVEDPKHPGQLDDKVFKGKAMTYYGRWTYKFEMASKLGADGVLIVHETIPAAYD
                     WSVVEHSWSGEAFVLADANGNAHSVPVQGWITLDKARALFAQSGQSFEALKVKAASRE
                     FKPVALNATVSVSIQNKLRDVHSHNVVASLPGSDPKLSHEWIVYTAHWDHFGRNPSLS
                     GDQIFHGAVDNGSGVASILEIARVFSETRPKPKRSILFMAVTAEEQGLLGSKYYAEHP
                     LHPLNETLADLNIDSVGTWGRARDLQVIGFGQSSLDDDLAQVLKSEDRVMVPDQQPEQ
                     GGYFRSDQFSFARVGVPGLFLSPGVDVIGKPAGFGQQQRDEYEAHRYHSPADRVYPDW
                     DLSGAAQDDTDLYKIGLALSAAAHWPEWRADSEFRAIRIQSLATRSKAALDH"
     gene            complement(2892..4157)
                     /locus_tag="VNZ06_10745"
     CDS             complement(2892..4157)
                     /locus_tag="VNZ06_10745"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 family protein"
                     /protein_id="HWW21273.1"
                     /translation="MRRHIWSVLLGVCVLTGCGGGGGSGGGVSTPPPAVLTNLTALTV
                     GPGPNGQNNINIPYANVMICQPNTSTCATINGLLVDTGSYGIRVLASALASAGLTLPN
                     TADPANANNTLAECIPFVDGYTWGPLATATIKINGETASSIPINVLNDNNTYNPTVPT
                     ACTSLTSNTSLDSLAALHANGILGVGIFPDDCGQGCETCASAGGGCTDQNDVYYSCNS
                     TANVCTPTPVPETAQTRNPVVSFTTDNNGVIIELPSIAASGSASVSGSLIFGIGTQSN
                     NGLGGAAVLTLSQNSFFTTTFNGQTLNSSFIDSGSNAYYFADASLATCSGNTDFYCPA
                     STVTLSATNQGGNGIMSNVSFQVANLNTLSNTNFAFNDVAGTAATSTGTDMLNNDFDF
                     GLPFFFGRHVFIAIDGAAAGGTTGPYYAY"
     gene            complement(4171..4629)
                     /locus_tag="VNZ06_10750"
     CDS             complement(4171..4629)
                     /locus_tag="VNZ06_10750"
                     /inference="COORDINATES: protein motif:HMM:NF022458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HWW21274.1"
                     /translation="MRLKPQLLGPALAILLWQAPAFAALGGDVRSVNADRAMMHGQLQ
                     TTSMQQYDLHEITTSGGTLIREYVTPHGQVFAVTWRGPFPPNLQQLFGSYYKQYQSAA
                     ASNATLGMHRIFSVTQSDFVAQALGRMRAYHGKAYVPSLVPSGVFLAALQ"
     gene            complement(4775..5785)
                     /locus_tag="VNZ06_10755"
     CDS             complement(4775..5785)
                     /locus_tag="VNZ06_10755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HWW21275.1"
                     /translation="MRISLLLISVGLAIASAQSHAAGTASMQAAVVTGSTLRIQAVPR
                     PSPAAGEVLVRLQYASVNPADWKRASGKPEDPSIGRPRDGQPAIPGLDAAGIIAALGP
                     AVSGFTVGDAVLLWSRHGGTYAQYVAVSAKDVIRRPDDLSPEKAAAIPHAGLAAWGML
                     MDIARVHSGQNVLVLGGAGGVGSAAVQIARMQGAHVTATASARNAAYLHQIGADRVID
                     YNSQHFEELVRNVDVVVNAVDADNAYRGLAVTKRGGYLVSVAGLPEAAQCAGRGVICV
                     ARDPALTSTRRALGQLADWARDGRFSVNIDRTFELKDVLQAWGYSQAGHTRGKAIIHL
                     GG"
     gene            complement(5849..6469)
                     /locus_tag="VNZ06_10760"
     CDS             complement(5849..6469)
                     /locus_tag="VNZ06_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWW21276.1"
                     /translation="MRHLLQSATALTAMAVTLMLATMPAHAQQSTGPLYGEGPYMGPY
                     VGGGVGQYNVRIDNAQEFGNVINHYSANDTAFKVFAGYRFAPFIALEGSYINLGTNRN
                     VIAPGVVAGSRIDGWSPELVATLPIGWFELFAEGGDYIYRYRRNLDVPGASFGSSSDT
                     FNHVEWGAGLGVVVARVIPIRLEYQEFNIQDTNRSNALWLTGAFRF"
     gene            6604..7686
                     /locus_tag="VNZ06_10765"
     CDS             6604..7686
                     /locus_tag="VNZ06_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWW21277.1"
                     /translation="MEKSDAQAQRPDWRAILAELGGLSQSMRGGPGYRGPRSDHFDGE
                     RFFNPGARSGKSFGEFLRWQRTRAQVPWPAWRAYQVVANPPSSVNVGDVAVTFINHMT
                     CLVQMGGFTLITDPVYSMRASPVQWAGPKRVHAPGLPFERLPRIDVVFVSHNHYDHLD
                     LPTLKRLARSQAALFVTGLGNGSFLREQGITHVLELDWWGQAILPHAILMFTPAQHWS
                     SRGLSNRNRTLWGGLWVSAGERTLYFSGDTGYTPLFAQLRRHRGSPDLALLPIGAYEP
                     RWFMCDQHMNPQEAVRAHMDLGARNSVGTHFGCFQLTDEGIDEPVIDLDRARREQGLT
                     AGEFYAPQPGETLLWRPGARALHVVA"
     gene            7683..8120
                     /locus_tag="VNZ06_10770"
     CDS             7683..8120
                     /locus_tag="VNZ06_10770"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HWW21278.1"
                     /translation="MNALRRITFAALLGATTLAAAASSTIQITDAWIRWLPGNLPAGG
                     YLTVINSGDHPVGLIGASSPDYARVTLHQSRQQGGVASMVPVGAVTILPHSTLAFEVK
                     GYHFMLEQPKKPLKPGDHVIITLQFDSRSSIAVQFEVRAAAAS"
     gene            8180..>8863
                     /locus_tag="VNZ06_10775"
     CDS             8180..>8863
                     /locus_tag="VNZ06_10775"
                     /inference="COORDINATES: protein motif:HMM:NF023863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HWW21279.1"
                     /translation="MSAQPAANLFSTSAAQGGAEAATRLREIPYNYTSFSDREIVVRL
                     LGEGMWALLDELRHERRTGRSARMLYEVLGDVWVVQRNPYLQDDLIDNPKRRRLLVSA
                     LYHRLAEVDGRRNAGADAERFVRVGELLEAVRAAVRSFEMQFAAWVQLRRKARRALAR
                     HTRADNIRFDAYARACHVTDATDWRVEYPFVVLMPDSEAQIPGLVRDCIELGFTLIPR
                     GGGTGYTGGA"
BASE COUNT         1643 a         2700 c         2882 g         1638 t
ORIGIN      
        1 cacgtgttta catcccatgg gcgtgccggc gaccggcttg agcagaaatg gcggcggtgc
       61 cgatgacgag gatgtagatg aaaattgcga tcgtttccga tgcctggcga ccgcagatca
      121 acggcgtcgt cacgacctta ggtaagaccg ctgaggccgc cgagcggctc ggccacacgg
      181 tcgaagtgat cagcgccgac ggatcggctt ccatcgcctg cccgagctac cctgagattc
      241 gcctggcgat ccgtccgggg cgcttggtgc gccagcggct gcgccagttc gcgccggaaa
      301 tgattcatat cgcgaccgaa ggtcctctgg gactggcggc gcgcaaccac tgcgtccgaa
      361 gcaagctcac gttcacgact tcctatcaca cccagtttcc ggactatgcg aatgcgcgct
      421 ggggtgtgcc ggcctcgtgg gggtattcgt atctgcgctg gtttcataag cccgcggtac
      481 ggaccctggt tggaaccgaa accgttcgca ccaatctgca tgcctgggga ttcagccatc
      541 tggtgccctg gtcgcgcggc gtggatacga cgctgtttcg ccccgatccg caagcgcggg
      601 cgatggcgaa cgcgcgctgg ccacgtccga tcatgttgta ctccggccgc gtagccgtcg
      661 agaagaacct cgaagcgttt ctgcgcttgc agctgcccgg aaccaagctg attgtgggtg
      721 acgggccggc acgggcaacg cttcagagcc gctacccaga cgccgtgttt ctgggctatc
      781 gcttcggcgc cgaactggcg tggcatatgg cagcggccga cgtcttcgtc tttccgagcc
      841 gtaccgatac attcggaatc gtgatgctcg aagcgatggc ctgcggtgtt cctgtcgcgg
      901 cctacccggt gcagggaccc ctcgacgtgg ttcgcgacgg cgttaccggc gtgctcgatg
      961 aggacctggc cagtgcggtg cagcgcgcct tgcgcctcga tacgaagcaa tgccgcgcgt
     1021 tcgctcagct gcagagctgg gaacgttgca cccgtcaatt catcgggcat ctggcgccgg
     1081 cctgcgagcg ccccgccctg cagcggctgc gcgcgcagga tcagggtgcc gcagttgcgc
     1141 agctgaagaa aggctgagcg cggccgcaag cgcaccctca tcgattgggg taggctctcg
     1201 accccatgaa acaccgaaca ttgctgctat ccctgtcgat gggcgctgcc tgtgccgtat
     1261 ctgctgccac cgctcctgct gccggggtcg atccggcgcg tctgcttcag cacatcaagg
     1321 tgctggaatc ggatgagttc gagggacgcc taccgggtac cgtcggtgag cagaagagcg
     1381 tcgcctacat caccggacag ttcaaacaac tgggcctgac tgcgggcaat cccaacggca
     1441 cgtacctgca ggatgtaccg ctggaaggaa tcgccggctc accctcgatg gcactgacgg
     1501 ccggtgggcg ggcgattccg atggaacacg gccacgattt cgtcgccagc acctatcgca
     1561 tcgtgccgca ggtattgatc agcaattcgc cgctggtgtt cgtcggttac ggtgtgcagg
     1621 ccccggaata caactgggat gactacaagg gtctggacgc gcatggcaag actctggtga
     1681 tgctgatcaa cgaccctccg gtggaggatc cgaaacaccc ggggcagctc gatgacaagg
     1741 tcttcaaagg caaggccatg acctactacg gccgctggac ctacaagttc gaaatggcgt
     1801 ccaagctggg cgccgacggc gtgctgatcg ttcacgaaac gattccggcc gcttacgact
     1861 ggtcggtggt cgaacactcg tggagcggtg aagcgtttgt gctggccgat gccaacggca
     1921 atgcccacag tgttccggta cagggctgga tcacgctcga caaggccaga gcgctgttcg
     1981 cgcagtccgg tcaaagtttc gaggcactga aggtcaaggc cgccagccgc gagttcaagc
     2041 cggtcgcgct caatgcgacc gtgagcgtct cgatccaaaa caagctgcgc gacgtgcatt
     2101 cacacaacgt cgtggcatcg cttccgggca gcgatccgaa actctcgcac gagtggatcg
     2161 tctacaccgc gcactgggat cacttcggac gaaatccgtc gctatcgggc gatcagatct
     2221 tccacggcgc ggtcgacaac ggctctggcg ttgccagtat tctggagatc gcgcgcgtgt
     2281 tcagtgaaac ccggccgaaa ccgaaacgct ccattctgtt catggcggtc accgcggagg
     2341 agcagggcct gctgggctca aagtactacg ccgaacatcc gctccatccg ttgaatgaaa
     2401 ccctcgcgga tctgaatatc gacagcgtcg gcacctgggg ccgcgcccgc gacctgcagg
     2461 tcattgggtt tgggcaaagc agcctggatg atgatctggc acaggtgctg aagagcgagg
     2521 accgtgtgat ggtcccggat cagcaacccg aacagggtgg ttatttccgt tccgaccagt
     2581 tcagcttcgc gcgcgtcggc gtaccgggcc tgtttctaag tccgggagtg gacgtcatcg
     2641 gtaagcccgc aggtttcggc caacagcagc gtgacgagta cgaagcgcat cgctatcact
     2701 cgccggcaga tcgggtgtac ccggattggg atctgtccgg cgcggcgcag gatgacaccg
     2761 acctgtacaa gatcgggctg gcgctatcgg cggcggcgca ctggccggaa tggcgcgcgg
     2821 attccgaatt ccgcgcgatc cggattcagt cgctggccac gcgcagcaag gccgcgctgg
     2881 accactagcg cctagtaagc gtagtaaggc ccggtggtgc cacccgccgc ggcgccatcg
     2941 atcgcgatga acacatggcg gccaaagaag aacggcaagc cgaagtcgaa gtcattgttc
     3001 aacatgtcgg taccggtgct cgtggccgcg gttccggcca cgtcgttgaa tgcgaagttg
     3061 gtgttggaca gcgtattcag attggcgacc tggaacgaga cgttgctcat gatgccgttg
     3121 ccgccctgat tggtagcgga gagcgtgacg gtcgaggccg ggcaatagaa gtcggtattt
     3181 cccgagcagg tcgcaagcga agcgtctgca aaatagtagg cgttcgagcc agagtcgatg
     3241 aagctgctgt tgagcgtctg gccgttgaaa gtcgtggtga agaacgagtt ctgactcaac
     3301 gtcagcaccg cagcaccgcc cagcccgtta ttgctttgcg tgccgatgcc gaatatcagc
     3361 gagccgctga cggatgccga gcccgaggct gcaatggacg gaagttcgat gatcacgccg
     3421 ttgttgtcgg tggtgaagga caccaccgga ttgcgcgtct gcgcagtctc cggcaccggg
     3481 gtcggtgtgc agacattcgc agtcgaattg cagctgtaat agacgtcgtt ctggtcggtg
     3541 caaccgccgc ccgcagaggc gcaggtctcg cagccctggc cgcaatcgtc ggggaaaatg
     3601 ccgacgccca gaatgccgtt cgcatgcagt gctgccagcg aatcgagcga tgtgttggag
     3661 gtcagtgacg tgcaggctgt gggaaccgtg ggattgtagg tgttgttgtc gttcagcacg
     3721 ttgatcggaa tgctgctcgc cgtctcaccg ttgatcttga tggtcgcggt cgcgagcgga
     3781 ccccaggtgt agccgtcgac aaacggaatg cactcggcca gcgtgttgtt cgcattggcg
     3841 ggatcggccg tgttcggcag cgtcaggccc gccgacgcca gggccgaggc cagcacacgg
     3901 attccgtagg agcccgtatc gaccagcaga ccgttgatgg ttgcgcaggt gctggtattg
     3961 ggctgacaga tcatgacgtt ggcgtacgga atattgatgt tgttctgacc gttcgggccg
     4021 ggtccgaccg tcagtgcggt cagattggtg agtaccgcag gaggcggggt cgacacgccg
     4081 ccgccgctgc cgccaccacc gccgcatccg gtcagaacgc aaacgccgag caacacgctc
     4141 cagatatgac gtcgcatcgt aagcctcact ttattgaaga gcagcgagga agaccccgga
     4201 gggcaccaga ctcggcacgt acgccttgcc gtgataggcg cgcatgcgtc ccagggcctg
     4261 cgccacgaag tcggactgcg tcacgctgaa tatgcggtgc atcccgaggg tcgcgttgga
     4321 cgcggccgcg ctctgatact gcttgtagta ggaaccgaac agctgctgaa gattcggtgg
     4381 aaaaggtccg cgccaggtga cagcgaacac ctggccgtgg ggcgtcacgt actcgcgaat
     4441 cagcgtgccg ccgctggtgg tgatctcgtg cagatcgtat tgctgcatcg aagtggtctg
     4501 caactggccg tgcatcatgg cgcgatcggc attgaccgag cgcacatccc cgccgagcgc
     4561 ggcgaaggcc ggtgcctgcc aaagaaggat cgccagtgcc ggaccgagta gctggggttt
     4621 caatctcatg gtgcagtcac ccgcgaagcg aggttgttag gaggatagct tgccaaaatt
     4681 gtcgcgcttg acatgagacg gcaatcactt tcgtcgaagt tgccgtcagg aaactgacgc
     4741 catctggcga cagtgagtcc atccgggagg cattctatcc gccaaggtgg atgatcgctt
     4801 tcccacgtgt atggccagcc tggctatacc cccacgcctg caggacatcc ttcagttcga
     4861 aggtccggtc gatattaacg gagaatcgac cgtcgcgcgc ccagtctgca agctgcccaa
     4921 gcgcccttcg ggtcgaggtc aacgcaggat ctcgtgccac gcaaatgacc cctcggccgg
     4981 cgcactgcgc agcctccggc aggccggcaa ccgacaccag atagccccca cgcttcgtga
     5041 ctgccagccc ccgataagcg ttgtcggcat ccaccgcgtt gacaacgacg tccacatttc
     5101 gcaccagctc ctcgaaatgc tggctgttgt agtcgatgac gcgatcggct ccgatctggt
     5161 gaagataggc ggcattgcga gccgatgcag tcgctgtgac atgtgcgcct tgcattctgg
     5221 cgatctgcac ggcggcggaa ccgaccccgc ccgcaccgcc caacaccagg acattctggc
     5281 cgctgtgtac ccgggcgata tccatcagca ttccccaggc ggcgagcccc gcatgtggaa
     5341 tggcggccgc cttttccggc gacaaatcat cgggccttcg tatcacgtcc tttgccgata
     5401 cggcgacgta ctgagcgtac gtcccgccgt gccgcgacca cagcagtacc gcatcgccca
     5461 ccgtgaagcc gctcactgca ggcccgagcg ccgcaatgat tccggccgca tcgagtcccg
     5521 gaatcgcggg ctgcccgtcg cgcggccttc cgatgctcgg atcctcgggt ttgccgctgg
     5581 cgcgcttcca gtcggccggg ttgaccgagg cgtactgcag tctgaccaat acttcaccgg
     5641 cggcggggct cgggcgcggt acggcctgta tgcgaagggt gctaccggta acgaccgcgg
     5701 cctgcatgct cgctgtgccg gcggcatgcg attgtgcgga tgcgatcgca agcccgacac
     5761 tgatgagaag aagcgagatc cgcaatggta agcaccctcc cgcgacatcc tgccacggaa
     5821 gggtctattc atgaaatgcc gcccgacttc agaatctgaa ggccccggtc agccataggg
     5881 cattggagcg gttggtgtcc tgaatgttga attcctgata ttccaggcgg atcggaatca
     5941 cccgagcgac caccacgccc aaccccgcac cccactcgac atgattgaat gtgtcgcttg
     6001 aggagccgaa actggcgccg ggaacatcga gattgcgccg atagcgatag atgtagtcgc
     6061 cgccttcggc aaacagttcg aaccagccga tcggcagcgt tgccaccagc tcgggcgacc
     6121 agccgtcgat gcgactgccg gcaaccacgc ctggcgcgat gacgttgcgg ttggtgccca
     6181 gattgatgta gctgccctcg agcgcgatga acggcgcaaa acggtaaccg gcgaagacct
     6241 tgaaggcggt gtcgttggcg gagtagtgat tgatgacgtt gccgaactcc tgggcattgt
     6301 cgatgcgaac attatattga ccgacgccac cgcctacgta cggacccata tagggtccct
     6361 cgccgtagag agggccggta ctctgctgcg catgggccgg catcgttgct agcatcagcg
     6421 tgacggccat cgcggtgaga gccgtagcgg actggagcaa atgacgcatg tgccgttcct
     6481 cgttcggtag aaggggtgcc agtgttggtc aagcggatcg cgcgcatcag tgcgcgaacg
     6541 gctgctctgt gcgtcggaga ttggctgtgc cgctgtaggc cccttgcccg taggaggacg
     6601 ctgatggaga aaagcgacgc ccaggcccag cggcccgact ggcgcgcgat actcgcggag
     6661 ctaggcggcc tgtcgcagtc gatgcgtggt ggaccggggt accggggacc gcgcagcgat
     6721 cacttcgatg gtgagcgctt cttcaatccc ggagcgcgct ctggcaagag cttcggggaa
     6781 tttctgcggt ggcagcgaac acgagcgcag gtgccgtggc cggcgtggcg agcataccag
     6841 gtggtcgcca acccgccctc gagcgtcaat gtgggcgacg tggccgtgac gttcatcaac
     6901 cacatgacgt gcctggtgca gatgggcggc ttcacactga tcactgatcc ggtctactcg
     6961 atgcgcgcca gccccgtaca gtgggccgga ccaaagcgcg tgcacgcacc cggcctgccg
     7021 ttcgagcgct tgccacgcat cgatgtcgtg ttcgtgagcc acaatcacta tgatcatctg
     7081 gacctgccaa cgctgaaacg tctggcgcgc tcgcaggctg cgctgttcgt caccggactg
     7141 ggcaatggca gtttcctgcg cgagcagggc atcacgcatg tcctggagct ggactggtgg
     7201 ggtcaggcga tcctgccgca cgccatcctg atgttcacgc cggcgcagca ctggtcgagt
     7261 cgcggattga gcaaccgcaa tcgcacactc tggggaggac tgtgggtgag cgcgggcgag
     7321 cgcacacttt atttttcagg agacaccggg tacacgccgc tgttcgcgca gctgcgccgt
     7381 caccgcggat cgccggatct ggcgctgctg ccgatcggcg cttatgagcc gcgatggttc
     7441 atgtgtgatc agcacatgaa cccgcaggaa gcggtacgcg cccacatgga tctgggcgct
     7501 cgcaacagcg tcggcacgca cttcggctgc ttccagctca ccgacgaagg catcgatgaa
     7561 ccggtcatcg atctggaccg tgcgcggcgc gagcagggac tcactgcggg cgagttctac
     7621 gcaccccagc cgggagagac cttgctgtgg cgtccgggcg cacgtgcgct gcacgtggtc
     7681 gcatgaatgc gctgcgccgt atcacgttcg cagcgctgct cggtgcgacc acgctggcgg
     7741 ccgccgccag cagtacgatt cagatcacgg acgcgtggat ccgctggctg ccgggcaacc
     7801 tgccagcagg cggctatttg acggtgatca attccggtga tcacccggtc ggtctgatcg
     7861 gagcatccag tccggactat gcccgcgtga cactgcatca aagccggcaa cagggcggcg
     7921 tggccagcat ggtgcctgtg ggcgccgtga cgattttgcc gcactcgacg ctggccttcg
     7981 aagtgaaggg ctatcacttc atgctcgagc agcccaaaaa accgctcaag ccaggcgatc
     8041 acgtgatcat cacacttcag tttgattcgc ggtcatcgat agcggtgcaa ttcgaggtac
     8101 gggctgcagc cgcgtcatga gcttatgagg acccagcggt cgcgggcggg tgagcccgcc
     8161 ggggtagaat gcccaggcaa tgagcgcgca accggcggcc aatctgttca gtaccagcgc
     8221 ggcgcagggt ggtgccgaag cggccacgcg cctgcgcgag attccttaca actacacgtc
     8281 gttctcagat cgggaaatcg tggtgcgtct gctcggcgag ggcatgtggg cactgctgga
     8341 cgagttgcgc cacgagcgcc gcaccggccg ctcggcccgg atgctgtatg aggtgctggg
     8401 tgatgtctgg gtcgtgcagc gcaatcccta tctgcaggat gacctgatcg acaacccgaa
     8461 gcggcgacgg ttactggtgt ccgcactcta tcaccgcctg gccgaagtgg atggtcgccg
     8521 caatgccggt gcagatgccg agcggtttgt gcgcgtcggt gaactgctgg aagcggtgcg
     8581 cgcggcggtg cgcagcttcg aaatgcagtt cgcggcctgg gttcaactgc ggcgcaaggc
     8641 gcggcgcgca ctggcccgcc acacgcgcgc ggacaacatc cgcttcgatg cctatgcgcg
     8701 ggcctgccac gtcaccgatg cgaccgactg gcgcgtggaa tacccgttcg tggtgttgat
     8761 gcctgactcc gaagcacaga ttcccggact ggtacgcgac tgcatcgagc tgggtttcac
     8821 gctcattccg cgcggcggtg gtaccggata taccggcggg gcg
//
LOCUS       DATMRM010000357         2816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2372328, whole genome shotgun sequence.
ACCESSION   DATMRM010000357 DATMRM010000000
VERSION     DATMRM010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2372328"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VNZ06_10780"
     CDS             <1..287
                     /locus_tag="VNZ06_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HWW21280.1"
                     /translation="HEILGEQVREQLIYYPTVTRELFSNSGRITNLIDAGTLFSDTGL
                     PPFSPERDRIMLCGSSATLRDTRALLEACGFDEGSQSAPGHYVVERAFVS"
     gene            346..906
                     /locus_tag="VNZ06_10785"
     CDS             346..906
                     /locus_tag="VNZ06_10785"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HWW21281.1"
                     /translation="MNRIKTLFATALVAVASASALAAPTDPAGFWTTPTIQGYGNMHY
                     LPDSAFKPQPGHTYKVVFALTEAAAQPDKANPALDHVARTVNLYAASGVPLAKLKFVA
                     VAYGAATPIALDDAHYRAAFGVPNPNLPLIAELKKAGVTIAVCGQAVAEHHFQYDWIN
                     RDVTLALSGLTTVTALQQQGYALMQL"
     gene            1026..2012
                     /locus_tag="VNZ06_10790"
     CDS             1026..2012
                     /locus_tag="VNZ06_10790"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWW21282.1"
                     /translation="MSAIGALIIGSAHAEGEPAPGTAGIAHSQGVYPPWQHGANNDSP
                     VRGLSFTEPDADNLADFHGDPVHPSLALYVGGNYFFAMAPLVQAFEARYPQYKGHVYW
                     ETIPPGLLVKQMADGGTVTVGNMTWTVPADVYLAGLKAVQKQIDVGRLQAPAVPYVTN
                     TLTIMVPHGNPAHISGLADLGRPEVRLAMPNPKFEGIARQIKTSLEHAGGDTLLNEVY
                     GTKVADGSTVLTYIHHRQTPLWIMQGKAQAGVTWQSEAMFEEQAGHPISHVDIPAADN
                     TTAIYAGAMTTNAPHAEAARRWLDFIRSPEGLTIFERYGFKPYQADANANAG"
     gene            complement(2098..2439)
                     /locus_tag="VNZ06_10795"
     CDS             complement(2098..2439)
                     /locus_tag="VNZ06_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21283.1"
                     /translation="MHDVGFGAAEHLLHLAGRALQDRNYDLWKARPEPCDRIGQQIAS
                     VRMSGGDDQFPLIVIHIVRAEAFQGFDLAKNPLGCRNDQFALVGDSTQPLAVARKDLD
                     AQFVFEFNDRH"
     gene            complement(2540..>2816)
                     /locus_tag="VNZ06_10800"
     CDS             complement(2540..>2816)
                     /locus_tag="VNZ06_10800"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWW21284.1"
                     /translation="HIGLQIDPLMSVSDTEAIKRMLLVQHAIAYVSLLSVKDELRRGE
                     LTVLEVDDLRIERPLHMVWLKGRSLSPSTQGFFDLVLARVAADAAAR"
BASE COUNT          565 a          910 c          833 g          508 t
ORIGIN      
        1 aacacgaaat cctcggtgag caggtgcgtg agcaactgat ctactaccca accgtcactc
       61 gcgagctgtt ttctaacagc ggtcgcatta ccaatctgat cgacgccgga acgttgttct
      121 ccgacaccgg actaccgcca ttttcgcccg aacgcgaccg catcatgctg tgcgggagct
      181 ccgcgacgct gcgcgacacg cgcgccttgc tcgaggcatg tggcttcgat gagggcagcc
      241 agtcggctcc tggacactat gtggtcgagc gggccttcgt ttcatgaccc gggcggcaag
      301 ccagcgtcat tcacctttga tacatcgcca ttgagagaat tagtcatgaa cagaatcaaa
      361 actcttttcg ctaccgcgct cgttgcggtt gcctctgcgt cagcgctcgc agcgccgacc
      421 gatccagccg gcttctggac gacacccaca attcagggct acggcaacat gcactatctg
      481 cccgacagcg cgttcaaacc acagcctggc catacctaca aggtggtctt tgcgctgact
      541 gaggccgccg cacagcctga caaggccaac ccagcgctgg atcacgtggc gcgcacggtc
      601 aatctctacg ccgcttcagg tgtaccactg gcgaaactga aattcgtagc tgttgcgtat
      661 ggcgcggcga caccgatcgc gctcgacgat gcgcactacc gggcagcgtt tggcgtgccc
      721 aaccccaatc tcccgctgat tgccgagctc aagaaggccg gcgtgacgat cgcggtatgc
      781 gggcaggcag tggccgagca tcatttccag tacgactgga tcaatcggga tgtcacgctt
      841 gcgctgtccg gcctgaccac tgtcactgcg ctccagcagc agggttacgc gctgatgcaa
      901 ctgtaactgc acgttcgtcg cgcgcgcggg tggtgcgaac tgccgtgcag tgcgcgtacc
      961 ttcgttgatc atctggagtc cactgtatgt caaccatcaa ccgaaacacg cttgccttgt
     1021 gcatgatgag cgccatcggc gccctcatca tcggatcagc ccatgcggag ggcgagccgg
     1081 cacccggcac cgccggcatc gcgcatagcc agggcgtcta tccaccgtgg caacacggtg
     1141 cgaacaatga cagtccggta cgcggcctgt cgttcaccga acccgacgcg gacaatcttg
     1201 ccgatttcca tggcgacccg gttcatccgt cgctcgcgct ctatgtcggt ggcaactact
     1261 tctttgcgat ggcgccgctc gtgcaggcgt tcgaagcgcg ttacccacag tacaaaggcc
     1321 acgtctactg ggagacgatt cccccgggcc tgctggtgaa gcagatggca gacggcggca
     1381 cggtcacggt cggcaacatg acctggactg tcccggctga cgtgtatctc gctgggctga
     1441 aggctgtgca gaagcagatt gacgtcggac gcctgcaggc gccggcggtg ccgtatgtga
     1501 cgaatacact gaccatcatg gtgccgcacg gcaaccctgc ccatatctca ggactcgccg
     1561 atctcggccg gcccgaggtt cggctggcga tgccgaatcc caaattcgag gggatcgcac
     1621 ggcagatcaa gacgtcgctc gaacatgcgg ggggcgacac gctgctcaac gaagtctacg
     1681 gcaccaaagt cgccgacggg tcgactgtgc tgacgtacat ccatcaccgg cagacgccgc
     1741 tgtggatcat gcagggcaag gcgcaagcag gtgtcacatg gcagtccgag gcgatgttcg
     1801 aggaacaggc cggtcatccc atcagtcacg tggacattcc ggcggccgac aacacgacgg
     1861 cgatctatgc gggcgccatg acaacaaatg cgcctcacgc cgaggcggcc cgacgctggc
     1921 tggactttat ccgctcgccc gaagggctca caatcttcga acggtacggg ttcaagccct
     1981 accaggccga cgccaacgca aacgcgggct gaacgcgcac accatccgct tgcatcgcgt
     2041 gccgggtgtg ctcggtcgcc cggcatgcgc ccgtcttaca cgccacagcc cggcatctca
     2101 atgtcgatca ttgaactcga agacgaactg ggcgtcgaga tctttgcgcg ccacggcaag
     2161 cggctgagtg ctatcaccga ccagggcaaa ctggtcgttg cggcagccga gcggattctt
     2221 cgcgaggtcg aagccctgaa acgcctcggc gcgcactata tggatgacga tcagggggaa
     2281 ctgatcatcg ccgccactca tacgtacgct cgcgatctgc tgcccaatgc gatcgcacgg
     2341 ttcagggcgc gctttccaaa ggtcgtagtt tcgatcctgc aaggctcggc cagccaggtg
     2401 caagagatgc tccgcagcgc cgaagccgac gtcgtgcata cgctagtaga actgctgtgc
     2461 ccgatgtctt cgcgggaaca cgttgagcag gccatgcgaa acgcggaggc caccgtgaca
     2521 ggcctcgcgg ccaacaacgt caccgcgctg cggcatccgc tgcgacccgc gccagcacca
     2581 ggtcgaaaaa cccctgcgta ctcggcgata gcgaccgccc cttgagccac accatgtgca
     2641 gcgggcgctc gatgcgaaga tcgtccactt cgagcaccgt cagctcgccg cggcgcaact
     2701 cgtccttcac actgagcagc gaaacgtaag cgatggcgtg ctggacaagc agcatccgct
     2761 tgatggcttc ggtgtcgctg acggacatta gtgggtcaat ttgcagaccg atatgc
//
LOCUS       DATMRM010000358         3141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3595338, whole genome shotgun sequence.
ACCESSION   DATMRM010000358 DATMRM010000000
VERSION     DATMRM010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3595338"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VNZ06_10805"
     CDS             complement(<1..650)
                     /locus_tag="VNZ06_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase"
                     /protein_id="HWW21285.1"
                     /translation="MRRSASAKLAQALSETGVVEALSHEGAGIIHAGKAAFVPGALPG
                     ETVRFRRRRRHRQYDEAQLEEVLSPSSERVTARCQHFDVCGGCALQHLSAEAQLRLKQ
                     DQLRDALQRLGGVEPERWLPPIPGPAFGYRRRARLGSRYVDRKERVVVGFRERSSSLI
                     AAIERCEVLSPPVDALIAPLCALIGQLSIRRAVPQIEVAVGDDAVALVLRVLQSPSA"
     gene            complement(647..1423)
                     /locus_tag="VNZ06_10810"
     CDS             complement(647..1423)
                     /locus_tag="VNZ06_10810"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWW21286.1"
                     /translation="MNCLVFDIETVPDVELGRRLYGLQGLDDAAVAKAMFAIRRADSG
                     SEFLPHIQHRVVAIACALRTGDQLKVWSLGDLQSSETELLRRFFDGIEKFLPDLVSWN
                     GGGFDLPVMHYRSLKAGLQAATYWQTGDGDTAFRYNNYLSRFHWRHIDLMDVLAGFQQ
                     RARASLADTAALLGFPGKLGFSGDQVWDAYLSGQLLAVRRYCETDVLNTYLIYLRFQL
                     LRGQIDPSGLESEIERVRAMLRSSGEPHHAEFLRAWDAMV"
     gene            complement(1420..1857)
                     /gene="acpS"
                     /locus_tag="VNZ06_10815"
     CDS             complement(1420..1857)
                     /gene="acpS"
                     /locus_tag="VNZ06_10815"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HWW21287.1"
                     /translation="MIFGIGIDVLQAQRMKDSYARFGTHLVERLLLPQERVQFERTRR
                     PDRYLAMHFAAKEAIVKAMGTGFAHGVWIRDVGTVQNKWGRPQVIYSARGERLRRKLG
                     VGEGHVTLTDEAGLVVAVAVLLRATRRRGTTKPVKRRSKRTRA"
     gene            complement(1854..2585)
                     /gene="pdxJ"
                     /locus_tag="VNZ06_10820"
     CDS             complement(1854..2585)
                     /gene="pdxJ"
                     /locus_tag="VNZ06_10820"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014605257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HWW21288.1"
                     /translation="MHTDISLGVNIDHVATLRQQRHTVYPEPMQAALIAEQSGADNIT
                     LHLREDRRHIQERDVRMLAGLLTTRMNLEMAATPDMIALACEIKPVDCCLVPERRAEL
                     TTEGGLDVIAAHPALREHCARLAAAGIRVSLFIDAEHSQIEAARASGAPAIELHTGVY
                     ADTRGAAQARELERLRTAARFAAGLGLSVHAGHGMNYHNVTPIAAIPEITELNIGHAI
                     VARALFDGFATAVRTMKALMLAARA"
     gene            complement(2585..>3141)
                     /locus_tag="VNZ06_10825"
     CDS             complement(2585..>3141)
                     /locus_tag="VNZ06_10825"
                     /inference="COORDINATES: protein motif:HMM:NF014607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HWW21289.1"
                     /translation="SWSGRGEAPQLTGAEHAGAAYLLPPARLMSAFYLNELLIKLLTR
                     HDPHPQLFDCYEATLAQLAQQDQGDASLRQFEARLLDYIGYGLNLTAEADTGRPVRAE
                     AHYHFRPGVHGFVIAEPDSLGAIQGEVLRRLASGGGIESDSELRQARMLMRAALDHCL
                     EGRELATRAVARSLHHYPRKERLG"
BASE COUNT          560 a         1072 c          983 g          526 t
ORIGIN      
        1 gccgagggtg attgcagcac gcgcaacacc agggccaccg catcatcgcc aaccgccact
       61 tcgatctgcg gcacggcgcg gcggatcgag agctgtccga tcagtgcgca cagcggcgca
      121 atcagcgcat ccaccggcgg actgagcact tcgcagcgct cgatggctgc aatcaaactg
      181 gaactgcgtt cgcgaaaacc gacgaccacg cgttccttgc gatcgacata ccgggacccc
      241 agccgcgccc gacgtcgata gccgaaggcg ggtcctggaa ttggcggcag ccatcgttcg
      301 ggctcgacgc cgcccagtcg ctgcagagcg tcgcgcaact gatcctgctt cagtcgcagc
      361 tgcgcctcgg cggacaggtg ctgcagggcg cagccgccac agacatcaaa gtgctggcag
      421 cgcgccgtga cgcgttcgct gctgggactg agcacttctt cgagctgcgc ctcgtcgtat
      481 tgacggtggc ggcggcggcg gcgaaagcgc accgtctcgc cgggcagtgc acccgggaca
      541 aacgcggcct tgccggcgtg aatgatgccc gcgccctcgt gactgagcgc ctccaccacg
      601 ccggtctcac tcagcgcctg ggcaagcttt gcgctggccg aacgtctcat accatcgcgt
      661 cccaggctct caggaactcg gcgtgatggg gttcgcccga gctgcgcaac atcgcgcgca
      721 cgcgctcgat ctccgattcc agcccgctgg ggtcgatctg accgcgcaac aactggaagc
      781 gcaggtagat caggtacgtg ttcagcacat cggtttcgca gtagcgccgc accgccagca
      841 gttgcccgct caagtaggca tcccagacct gatcgcccga aaagccgagt ttcccaggga
      901 aaccgagcag tgcggccgtg tcggcgagcg aggcgcgtgc gcgctgctga aatcccgcca
      961 gcacatccat cagatcgatg tgccgccagt gaaagcgcga cagatagttg ttgtaacgaa
     1021 aggcggtatc gccatcgccg gtctgccagt aagtcgcagc ctgcagacct gctttgaggc
     1081 tgcgatagtg catcacgggc agatcgaaac caccgccatt ccatgacacc aaatccggca
     1141 gaaacttctc gatcccatcg aagaaccgtc gcagcaattc ggtttcgctc gactgcagat
     1201 cgcccaggct ccacaccttg agctgatcgc cggtcctgag cgcgcaggcg atcgcgacca
     1261 cgcggtgctg gatgtgcggc aggaactcgc tgcccgagtc agcgcgacga atggcaaaca
     1321 tggccttggc gaccgccgcg tcgtccagcc cctgcagtcc atacagacga cgaccgagtt
     1381 cgacgtccgg taccgtttca atgtcgaaca cgaggcagtt cacgcccgag tcctcttcga
     1441 ccgacgcttc acgggttttg tggtacctcg tcggcgcgtc gcgcgcagca gcactgcaac
     1501 cgccacgacc agaccggcct catccgtgag cgtgacgtgc ccctcgccga cgcccagctt
     1561 gcgccgcaag cgctcgccgc gcgcggaata gatcacttgc ggcctacccc acttgttctg
     1621 caccgtaccg acatcgcgga tccagacgcc gtgggcgaac ccggttccca tcgctttgac
     1681 gatcgcttcc ttggcggcga agtgcatggc cagatagcga tccggacggc gagtgcgctc
     1741 gaactgcacc cgctcctgtg gcagcagcag ccgctcgacc aggtgcgtgc caaagcgcgc
     1801 gtaactgtcc ttcatgcgct gcgcctgcag cacgtcgatc ccgatgccga agatcatgcg
     1861 cgcgcggcca gcatcagcgc cttcatcgtg cgcaccgcgg tagcaaatcc atcgaacagc
     1921 gcccgcgcca caatcgcgtg cccgatgttc agttcggtaa tctccgggat cgcggcgatc
     1981 ggtgtgacat tgtggtagtt catgccgtgg ccggcatgta cgctcaatcc cagtccggcc
     2041 gcgaagcgcg ccgcagtgcg cagccgttcg agctcgcgcg cctgcgcggc gccgcgagta
     2101 tccgcataca cgccggtatg cagttcgatg gccggggcac cgctggcgcg ggcggcttcg
     2161 atctgagaat gctcggcgtc gatgaacagc gagacgcgaa tgccggccgc ggcgaggcgg
     2221 gcgcaatgct cgcgcagcgc cgggtgcgcc gcgatcacat caagaccgcc ttcggtcgtg
     2281 agctcggcac ggcgttccgg caccaggcag cagtccaccg gtttgatctc gcaggccaga
     2341 gcgatcatgt cgggcgtcgc cgccatttcc agattcatgc gggtcgtcag cagtccggcc
     2401 agcatccgca cgtcgcgctc ctggatgtgc cgtcggtcct cgcgcagatg caacgtgatg
     2461 ttgtcggcgc ctgactgctc cgcgatcagg gcagcctgca tcggctccgg gtataccgtg
     2521 tgccgctgct ggcgcagcgt ggcgacatga tcaatattca cgcccagcga aatatcggtg
     2581 tgcatcaacc cagacgctcc ttgcgaggat agtgatgtag cgagcgggcg acggctcgcg
     2641 tggcaagctc acgtccttcc agacaatgat ccagcgcagc gcgcatcagc atgcgcgcct
     2701 ggcgcagttc actgtcgctc tcgatgccgc cgccggatgc cagccggcgc agcacctcgc
     2761 cctgaatggc gccgaggctg tcgggctctg caatcacgaa gccgtgcaca ccgggtcgaa
     2821 agtggtagtg cgcttcggct cgcaccggcc ggccggtatc ggcctcggcc gtcagattca
     2881 gtccgtagcc gatatagtcc agcagccgcg cttcgaactg acgcagtgag gcgtcgccct
     2941 gatcctgctg tgccagctgc gccagcgtcg cttcgtagca atcgaacagc tgcggatgcg
     3001 gatcgtgccg ggtgagcagc ttgatcagca gctcattcag atagaacgcg ctcatcagac
     3061 gcgcgggcgg cagcaggtag gctgccccgg catgttccgc gccagtgagc tgcggcgcct
     3121 cgccgcgccc gctccaggac a
//
LOCUS       DATMRM010000359         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1450440, whole genome shotgun sequence.
ACCESSION   DATMRM010000359 DATMRM010000000
VERSION     DATMRM010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450440"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VNZ06_10830"
     CDS             complement(<1..548)
                     /locus_tag="VNZ06_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HWW21290.1"
                     /translation="MTASSLAHADIAVLGAGSWGTALAIQFARAGRPARLWGRDRSHV
                     QAMQRERSNARYLPDVGFPDGLHLLDSLAAAVDGVNDVLLAVPSHAFRSLLDQLKPML
                     PASIRLCWATKGFELETGRLPNQVAHEVLGPGRSVAVLSGPTFAAEVGRGLPTAMTIA
                     SPDVDYAEKLARDLSSSSFRAYT"
     gene            complement(562..1041)
                     /gene="secB"
                     /locus_tag="VNZ06_10835"
     CDS             complement(562..1041)
                     /gene="secB"
                     /locus_tag="VNZ06_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016772114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HWW21291.1"
                     /translation="MTNVQVPPGPAPGTPATTTLVLQGVYLKDCSFEAPQGPRGNGEW
                     NPQISLDLNTSVQPLGGDTREVVLTVTVSAKQAEQTLFLVEVKQAGAFTMQNLSADEY
                     KRAVGSICPTVLFPYARAVVSQLVSQGGFPQLLLPPVNFDALYANSQNDAAAGATKN"
     gene            complement(1069..1329)
                     /gene="grxC"
                     /locus_tag="VNZ06_10840"
     CDS             complement(1069..1329)
                     /gene="grxC"
                     /locus_tag="VNZ06_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HWW21292.1"
                     /translation="MAAPQIVMYTKSWCPYCERARALLKAKGVTFEEIDIESHPERRE
                     EMIRRSGRRTVPQIFVGTRYLGGSDDIHELEAAGGLDALLHV"
     gene            complement(1329..1751)
                     /locus_tag="VNZ06_10845"
     CDS             complement(1329..1751)
                     /locus_tag="VNZ06_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWW21293.1"
                     /translation="MDRLLEYISHHPWLAAFAVAAAMAVLINELRQRALGFAALGPQD
                     VIRLMNQGATLLDMRAQQEYQQGHISGARHFEPGQLATASDTLKRYKEKALIVYCDRG
                     ASAGAAVRALTKQGFTKVFNLRGGIAAWRAENLPLARD"
     gene            1825..>2719
                     /locus_tag="VNZ06_10850"
     CDS             1825..>2719
                     /locus_tag="VNZ06_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21294.1"
                     /translation="MRANFSVLVLILLALWLPAAILDAATPKKTEAELRALNQKIERM
                     TREVSRDALERERQSASLRAAEVSLGQVRGELSRLSREYTDRATHRAALAQSRQQEQQ
                     ALARERAALAGQLRAAYMIGQQEPLKLLLNQQDPLHSGRLYVYYSYFSRARAQEIAQI
                     QQHVEHLNELDAELEQQQSELVGLKSAQQAQLGQLERARNDRQTVLASFNAAAHTREE
                     SLARLKTQQADLERLLKELDHSLKTVPPPDIATAFGRSRGQLNWPVAGRITAQFGAKR
                     ASGVSWEGVVIATERAAPVSAV"
BASE COUNT          496 a          877 c          878 g          468 t
ORIGIN      
        1 gtgtaagccc ggaagctcga tgatgacaga tcgcgtgcca gcttctcggc atagtccaca
       61 tccggtgagg cgatcgtcat cgcggtgggc agtccgcgcc cgacctccgc agcgaacgtg
      121 ggaccggaca gcaccgccac cgaacgtccc ggtcccagca cttcgtgtgc gacctgattg
      181 ggcaggcgtc cggtctcgag ttcaaacccc ttggtggccc agcacaatcg aatggaagcc
      241 ggcagcatcg gcttcaactg atcgagcaac gagcggaacg cgtggctggg cacggcgagc
      301 agcacatcat tgacgccgtc gaccgcggcg gccagcgaat ccagcaaatg cagaccatcg
      361 gggaatccga cgtccggcag atagcgcgcg ttgctgcgtt cgcgctgcat ggcctgcaca
      421 tgcgagcgat cgcggcccca cagccgggcg ggacgtccgg cccgcgcaaa ctgaatcgcc
      481 agcgccgtgc cccaggagcc ggcgccgagg accgcgatgt cggcatgcgc gagggagctt
      541 gccgtcacgg cccgactggc cttagttttt ggtggcgccc gcggcggcgt cgttctgcga
      601 gttggcgtac agcgcgtcga agttcaccgg cggcagcagc agctgcggaa agcccccctg
      661 gctgaccagt tgcgacacca cggcgcgcgc atacggaaac aacacggtcg ggcagatgct
      721 gccgaccgcg cgcttgtatt cgtcggctga cagattctgc atcgtgaagg cgccagcctg
      781 cttgacctcg accagaaaca gcgtctgttc ggcttgcttc gccgacacgg tgacagtcag
      841 cacgacttcg cgggtgtcgc cgccgagcgg ctgcacactg gtgttcagat cgagtgagat
      901 ctgcggattc cattcaccat tgccgcgcgg accctggggc gcctcgaacg agcagtcctt
      961 gagatagacg ccctgcagca cgagtgtggt ggtggcgggc gtgccaggtg cgggtcccgg
     1021 tgggacttgt acgttggtca tgtgaaataa accctagctt cagtgaattt aaacgtgcag
     1081 cagcgcatcg agtccgcccg ccgcttcgag ttcgtgaata tcgtccgaac cgccgaggta
     1141 tcgtgtgccg acaaatattt gcggcacggt tctgcgtccg ctgcggcgga tcatctcctc
     1201 tcgccgctcg ggatgcgact cgatatcgat ctcctcaaaa gtcacgccct tcgccttgag
     1261 cagcgcccgc gcccgctcgc agtacgggca ccacgacttg gtgtacatca caatctgcgg
     1321 cgcggccatc agtcgcgcgc cagcggcaga ttctcagcgc gccaggccgc gatcccgccg
     1381 cgaaggttga ataccttggt gaacccctgt ttcgtcagcg cacgcaccgc ggccccggcg
     1441 ctggcgccgc ggtcgcaata gacgatcagc gccttctcct tatacctctt gagggtgtcg
     1501 cttgcggttg cgagctgacc cggctcaaag tggcgcgcgc cgctgatatg gccctgctgg
     1561 tactcctgct gcgcgcgcat gtcgagcagc gtcgcaccct ggttcatcag ccggatgacg
     1621 tcctggggcc cgagcgcggc aaaccccagc gcacgctggc gcagctcatt gatgagcacc
     1681 gccatggcgg cggcgacggc aaacgccgcc agccagggat ggtggctgat gtattcgagc
     1741 agtcgatcca tagtctcaag tcgtagccta gccgcgcatt ataactgcta ttggaaacgg
     1801 ccttgtccta gacttcccgg cggtatgcgg gcaaatttct cggtcctggt tttgatactg
     1861 cttgcgctgt ggctgccggc cgcgatcctg gatgcggcga ccccgaagaa gactgaagcc
     1921 gaactgcgtg ccctcaacca gaagatcgag cggatgaccc gagaggtcag tcgcgacgcg
     1981 ctcgagcgtg aacgtcagtc cgcgagtctg cgcgccgccg aggtatcact gggccaggtg
     2041 cgtggtgagc tatcgcgcct gtctcgcgaa tatacggatc gtgccactca ccgggccgca
     2101 ttggcgcaga gccggcagca ggagcagcaa gcactggcgc gcgagcgtgc ggcattggcc
     2161 ggacagctgc gcgcggcgta catgatcggt cagcaggagc ctctgaaact gctgctcaat
     2221 cagcaggacc cgctgcacag cggccgttta tatgtctact acagctattt cagccgcgcc
     2281 cgcgcccagg agattgcgca gatccagcag cacgtcgagc acctgaatga actcgacgcc
     2341 gagctcgaac agcagcagtc cgagttggtc ggcctgaaat ccgcgcagca ggcgcaactc
     2401 ggccagttgg agcgcgcccg caacgatcgg cagacggtgc tggccagttt caatgcggcc
     2461 gctcacaccc gcgaggagag cctggcgcgc ctgaagaccc agcaggccga tctggagcga
     2521 ttgctgaagg agctggatca ctcactgaaa acggtacccc ctcccgacat tgccaccgca
     2581 ttcgggcgat cccgcggaca gctcaattgg ccggttgcgg gtcgaatcac cgcgcagttc
     2641 ggtgccaagc gagccagcgg ggtgtcctgg gagggagtgg tgatcgccac cgagcgcgca
     2701 gcgccagtga gtgcggtcg
//
LOCUS       DATMRM010000360         5470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3920452, whole genome shotgun sequence.
ACCESSION   DATMRM010000360 DATMRM010000000
VERSION     DATMRM010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3920452"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="VNZ06_10855"
     CDS             <1..770
                     /locus_tag="VNZ06_10855"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWW21295.1"
                     /translation="TGRPLSCASLEDRLTMGRIFEVRKHVMFARWNRMAKQFARVAKD
                     INMAVKAGGPDPNSNPALRRILQNARAVNMPKDKVEAAIKRACGRDATHYTEVHYEGY
                     APHGIALMVETATDNPTRTVAHVRNIITKNGGHLGTTGSVAFQFQKMGVFRLDPSGIN
                     QDELELDMIDHGLEEMGESTGEKGESQLVLRCAFESFGRLQAALEARKIAPISAEHEY
                     VCSTPITLPEAQANEALELIDKLEQEEDVQRVFHTLA"
     gene            complement(818..4399)
                     /gene="putA"
                     /locus_tag="VNZ06_10860"
     CDS             complement(818..4399)
                     /gene="putA"
                     /locus_tag="VNZ06_10860"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HWW21296.1"
                     /translation="MNTRDSERGAIERLWRAPEHECVAPLIAAAQLDDAQRERVASRA
                     RRLIEGMRRHGGAEGVEALTRAFPLNSSAGQALLSLAEALLRVPDTANSNRLLRDRLS
                     RVDWSEQPNAGTLARLLQLATGMVRESNPIAPLAMPIVRLMAQIAIKALSGQFVFAED
                     IDTALRRAHREPHDSFRYSFDMLGEAAMNDHDAQRYLAAYEHAIHAVGRADRGRGPVA
                     GGSISVKLSAIHPRYNYAQHDRAMQELLPRLLSLARLARRYNIALAIDAEESERLDLS
                     LDLIECVMAQQDLAEWSGFGVVVQAYQKRAPAVIEYLLRLAARRPARLMVRLVKGAYW
                     DAEIKLAQMEGLSDFPVYTRKVYTDVAYLACARALLRARDRVMPQFATHNAQTVAQIL
                     ELAEGVHRDDFEFQCLYGMGAGLYHEVMHTPEFARPVRIYAPVGTRPTLLAYLMRRLL
                     ENGAAASFVQRAASSDCSIEQLIADPVGQACALGGAPHPKIEPPREIFGTRRNSSGVD
                     LSSVAQRAVLIDAIEHSRAAAIAVAPLIAAPANAKASTARCGREIRNPADRKELIGTV
                     ADASAQDLEAALAAAKAATGQWASTDVEMRARVLERAADLFEQELPALVALAVRESGK
                     TLANAVGEVREAVDYLRYYPARLRAEFSQGGQHPLGVLACISPWNFPLAIFTGQVAAS
                     LAAGNCVLAKPAEQSSAMGACAAQLLHQSGVPAAVLQLLPGDGEKVGAALVADPRVDG
                     VVFTGSTAAARSIARTLAQRGDVALIAETGGQNAMIVDSTALPEQVVADVLRSAFDSA
                     GQRCSALRVLCLQQEIADTVLAMLEGAMRELRVGDPADIATDVGPLIDEEARSRIDAH
                     LQAMRSRIRTQSALPERCRDGVFVPPTLIEIQSIAELQAEVFGPVLHVLRFERRELVA
                     LVESINATGYGLTLGIASRIDSTIKEIVAQASVGNVYVNRNVIGAVVGLQPFGGHGLS
                     GTGPKAGGPLYLHRLMKSSPGYDWQTAQPGSVPAGLKQLHDWLERGHDVVLSAQERQQ
                     LAQQVARYQQRALCGVHVALKGYVGESDELRLRPRGVLRATARSVGALLAQFAAALAT
                     GNTLTVDQPELTSKLAAALPHGLRRALKGVASHYEAVLVDAGDARSNPLWLQQLYRDV
                     AALDGPIVPVLLSGEGYALDRLLLEQTVTINTAAVGGDVRLLALGDA"
     gene            complement(4402..4929)
                     /locus_tag="VNZ06_10865"
     CDS             complement(4402..4929)
                     /locus_tag="VNZ06_10865"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HWW21297.1"
                     /translation="MAEVEIHTAHGAHADGFGQAVGILVGIIGILLAIVTILSHREHT
                     AAVVHKTEANDQWAYYQAKKIREHTAQVGAGLLTALASDPAKVAAISGKFTQDSERYA
                     READAISAVARERDGQSRHAEDRAIRFDLGEGFLELGLVMSSLYFLSRKRFFVALGSV
                     AAAIGAAIGVIGLLA"
     gene            complement(5050..5409)
                     /locus_tag="VNZ06_10870"
     CDS             complement(5050..5409)
                     /locus_tag="VNZ06_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HWW21298.1"
                     /translation="MPNNNPQGHNQYTKRREPMQPSATAAEGPLSDLTTGEREAIDTT
                     STASPDTTIPSTSGASQGPAKRGFAAMDEATQRTIASKGGQSQGKENNPGNFANDRDR
                     AREAGRKGGEAHGHRQP"
BASE COUNT          960 a         1879 c         1707 g          924 t
ORIGIN      
        1 caaccgggcg gccgctgagc tgcgcgtcgc tcgaggatcg acttacgatg ggccggatat
       61 tcgaagtacg taagcacgtc atgttcgcgc gctggaaccg catggccaag cagttcgcgc
      121 gcgtcgccaa ggacatcaac atggcggtca aggcgggtgg ccccgatcca aattccaacc
      181 ccgcgctgcg ccgcatcctg cagaatgccc gcgcggtcaa catgcccaaa gacaaggttg
      241 aggcggcgat caagcgggcc tgcggacgcg acgccaccca ctacaccgag gtgcactacg
      301 agggctacgc gccgcacggg atcgcgctga tggtggaaac agcgaccgac aatccaacgc
      361 gcacggtggc gcatgtgcgc aacatcataa ccaagaacgg cggccatctg ggcaccaccg
      421 gcagcgtcgc attccagttc cagaaaatgg gcgtgttccg gctcgatccc tcaggcatca
      481 atcaggacga gctggaactg gacatgattg atcacggcct cgaggaaatg ggcgagagca
      541 ccggagagaa gggcgagtcg cagctcgtgc tgcgttgtgc gttcgagagt ttcggcagac
      601 tgcaggcagc actcgaagcg cgcaagatcg cgcccatctc ggccgagcac gaatacgtct
      661 gctcgacccc gatcacgctg cctgaggcac aggcgaacga agcgctggaa ctgatcgata
      721 agctggagca ggaagaagac gtgcagcgcg tcttccacac actcgcctaa gccatcaggc
      781 gctgattcga ctacccgcgc cggcgaggcg acggccttca ggcatcgccc agtgccagca
      841 ggcgcacgtc gccgcccacg gccgcggtat tgatcgtgac ggtctgttcc agcagcagcc
      901 gatcgagcgc gtagccctcg cccgacagca gtaccggcac gatcggtccg tcaagtgccg
      961 ccacgtcgcg atagagctgc tgcagccata gtgggttcga gcgagcatca cccgcatcca
     1021 ccagcaccgc ttcgtaatgg ctcgcgactc ctttgagtgc tcggcgcagg ccatgcggca
     1081 gagccgcggc cagtttggat gtcagttccg gctgatccac ggtaagcgtg ttgccggtgg
     1141 cgagcgccgc ggcgaactgt gccagcagtg cgccgaccga cctggcggtg gcgcgcagca
     1201 cgccgcgcgg ccgcaggcgc agctcatcac tctcaccgac gtagcctttg agcgccacgt
     1261 gcactccgca cagggcgcgc tgctgatagc gcgcgacctg ctgcgcgagt tgctgccgct
     1321 cctgggccga cagcacgaca tcatggccgc gctccagcca gtcgtgcagt tgcttcagtc
     1381 ccgccggcac gctgcccggt tgcgccgtct gccagtcata gcccggggag ctcttcatga
     1441 ggcgatgcag atacagcggt ccaccggctt tcgggccggt acccgacaga ccgtgaccgc
     1501 cgaacggctg caggccgacg accgcgccga tcacgttgcg attcacgtag acgttgccga
     1561 cgctggcctg cgcgacgatc tctttgatcg tgctatcgat gcgcgaggcg ataccgagcg
     1621 tcaggccata gccggtggca ttgatcgact cgaccagcgc gaccagctcc cgccgctcga
     1681 atcgcagtac atgtaacact ggaccgaaca cttccgcctg aagctcggcg atcgactgaa
     1741 tctcgatcag ggtaggaggg acgaacacgc cgtcgcggca ccgttccgga agggcactct
     1801 gagtgcgtat ccgagaccgc atcgcctgca gatgtgcatc gatgcgcgag cgcgcctcct
     1861 catcgatcag cggcccgaca tcggtcgcga tatcggccgg atccccgacc cgcagctcgc
     1921 gcatcgcccc ttccagcatc gcgagcacgg tatcggcgat ctcttgctgc aaacacagga
     1981 cacgcagcgc cgagcagcgc tgccccgccg agtcaaaggc gctccgcagc acatcggcca
     2041 ccacctgttc cggcagcgcc gtggagtcca cgatcatggc gttctggccg ccggtctcag
     2101 caatcagcgc tacgtccccg cgttgtgcca gcgtgcgcgc aatcgagcgg gccgcagcgg
     2161 tcgagccggt gaacaccacg ccgtccaccc gcggatcggc gaccagcgcg gcaccgacct
     2221 tctcgccatc gccgggcagc agttgcagga ccgcagccgg tacaccggac tgatgcagca
     2281 gttgcgcggc acacgcaccc atggcgctgc tctgttcggc aggcttggcg agcacgcagt
     2341 tgcccgccgc gagtgaggct gcgacctgac cggtgaaaat cgccagtgga aaattccacg
     2401 gactgatgca ggcgagcacc cccagcgggt gctggccccc ttggctgaat tcagctcgca
     2461 ggcgggccgg gtagtagcgc agataatcca ccgcctcgcg cacttcaccc actgcattcg
     2521 ccagtgtctt gccggactct ctgaccgcga gcgcaaccag tgccggcaac tcctgctcga
     2581 acaggtcggc cgcccgctcg agcacacggg cgcgcatctc cacgtcggtc gaggcccact
     2641 gcccggtcgc ggccttggcc gccgccaggg ccgcttccag atcctgcgcg ctcgcatccg
     2701 ccacggttcc gatcagctcc ttgcggtctg cggggttgcg aatctcgcgt ccgcaccgtg
     2761 ctgtgctcgc tttggcattc gcgggcgccg cgatcagtgg cgccaccgcg atcgccgcag
     2821 cacgactgtg ctcgatcgca tcgatgagca ccgcgcgttg tgcgacgctg ctcagatcca
     2881 cgccgctgga attacgacgg gtaccgaaaa tctcgcgtgg cggttcgatc ttcggatgcg
     2941 gggcgccgcc tagtgcgcac gcctgcccga ccggatcggc gatcaactgt tcgatcgagc
     3001 agtcgctgct cgccgcacgc tgcacgaacg aggctgcggc accgttttcc agcaggcggc
     3061 gcatcagata cgccaacagc gtcggccgcg ttccgaccgg tgcgtagatt cgtaccggtc
     3121 gcgcaaactc cggtgtgtgc atcacctcgt gatagagccc ggcgcccatg ccgtacaggc
     3181 actgaaactc aaaatcatcc cgatgtacgc cctctgccag ttccaggatc tgggccaccg
     3241 tctgcgcgtt atgcgtcgcg aactgcggca tgacgcgatc gcgcgcacgc agcaacgccc
     3301 gcgcgcatgc cagatacgcc acgtcggtat agaccttgcg ggtatagacc ggaaagtcgc
     3361 tcaggccctc catctgcgca agcttgatct cggcgtccca gtaggcgccc ttcaccagcc
     3421 gcaccatcag acgtgccggg cggcgcgccg ccaggcgcag caggtactcg atcacggcgg
     3481 gagcgcgttt ttgataggcc tggacgacga cgccaaagcc cgaccactct gccaaatcct
     3541 gttgcgccat cacgcattcg atcagatcga gcgacaggtc cagccgctcc gactcctcgg
     3601 cgtcgatcgc cagtgcgatg ttgtaccggc gcgcgaggcg tgcgagcgac agcaggcggg
     3661 gcagtagctc ctgcatcgcg cgatcgtgct gcgcatagtt gtagcgcgga tgaatcgccg
     3721 atagcttgac cgaaatactg ccgcccgcga ccggtccgcg gccgcgatcc gcgcggccga
     3781 ccgcgtgaat cgcatgctcg taagccgcaa gatagcgctg cgcatcgtga tcgttcatcg
     3841 ccgcttcgcc cagcatgtcg aacgaatagc ggaagctgtc gtgaggttcg cggtgcgctc
     3901 gccgaagtgc cgtatcgatg tcttcggcaa atacgaactg cccgctcaga gccttgatcg
     3961 cgatctgcgc catcaggcgc acgatcggca tcgccagcgg agcaatggga ttggattcac
     4021 gcaccatgcc cgtcgccagc tgcagcagcc gggcgagcgt cccggcattg ggctgctcgc
     4081 tccagtccac tcgcgacagt cgatctcgca gcaaacgatt gctgttggcg gtgtccggca
     4141 cgcgcagcag cgcttccgcc agcgacagca aggcctgacc ggccgaactg ttgagcggga
     4201 acgcgcgcgt gagggcttcg accccctcgg cgcctccatg gcgccgcatg ccctcgatca
     4261 gacgccgcgc gcggctcgcc acgcgttcgc gctgcgcgtc gtcgagctgt gccgccgcga
     4321 tcaaaggtgc tacacactca tgctcgggcg cgcgccagag ccgctcgatt gccccgcgct
     4381 cggaatcccg ggtattcact gctaggccag caggccgatc acaccgatcg ccgcaccgat
     4441 cgcagccgct acgctgccga gcgccacgaa gaatcgtttg cgggacagga aatagagcga
     4501 cgacatcacc agcccgagtt ccaggaatcc ctctcccaga tcgaagcgga tggcccgatc
     4561 ctccgcatgc ctggactgtc catcccgctc gcgcgccacg gcgctgatgg catcggcctc
     4621 tctggcgtat cgttcgctgt cctgcgtgaa cttgccgctg atcgcggcga ccttggccgg
     4681 atcgctcgcc agcgcagtca gcagcccggc gccgacctga gccgtatgct cacggatttt
     4741 cttggcctga tagtaggccc attgatcgtt ggcctcggtc ttatgaacca ccgccgcggt
     4801 gtgctcgcga tggctgagaa tggtgacgat ggccagcaga atgccgatga tcccgacgag
     4861 gatacccacc gcctggccaa atccgtcggc atgtgcgcca tgcgcagtgt ggatttcgac
     4921 ttccgccatg ctgactccgt gccacgcgcg cagccacgcg caaggcgcgc gattctagca
     4981 gacggactcg agcgctccag aacctacgcg ggagctggcg gcgctgccag ctcccgcgta
     5041 tcgataggac tacggttgcc ggtgaccgtg ggcttcacca cctttgcgtc cggcctcgcg
     5101 cgcccgatct cgatcattgg cgaaattgcc agggttgttc tccttgccct ggctctgtcc
     5161 gcccttgctg gcgatggtgc gctgggtcgc ctcatccatc gcggcgaatc cgcgtttggc
     5221 aggtccctgt gatgcaccgg aagtcgacgg gatcgtcgtg tccggggacg ccgtcgaggt
     5281 ggtgtcgatc gcctcgcgtt cgccggtcgt caggtccgat aatggaccct ccgccgccgt
     5341 cgccgacggc tgcatcggtt cccggcgctt ggtgtactga ttgtggcctt gaggattatt
     5401 gttcggcatg agatcgcgtc ctcctggaaa tagacatggc aagcctcctg ccgatggttc
     5461 ctcctggaac
//
LOCUS       DATMRM010000361         6657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1139278, whole genome shotgun sequence.
ACCESSION   DATMRM010000361 DATMRM010000000
VERSION     DATMRM010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139278"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VNZ06_10875"
     CDS             <1..305
                     /locus_tag="VNZ06_10875"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWW21299.1"
                     /translation="VVGYSGPQRPRQKFFPPEWAVVRMLSVLPAERGGGLGRLLTQAC
                     IDRARRDRVATIGLHTSPAMPAALGLYLRMGFALCQTLPEMRGVPYALYTRHLTDD"
     gene            343..897
                     /locus_tag="VNZ06_10880"
     CDS             343..897
                     /locus_tag="VNZ06_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HWW21300.1"
                     /translation="MATQHERTEVQALARYTRTAISLHWVMFLLMAAGFTIGAYMADM
                     HVSPLKARLFSYHKWIGVTVLGFALIRVLWRLTHQPPPNLPMPRWQQIAAHSVHWLLY
                     ALMIATPMVGWLYSSAAGFPIVYLKLWQLPDLVHKNEALAKVLVQVHGFLAWTLLWVV
                     ALHALAALKHHFFDRDATLKRMWS"
     gene            930..1478
                     /locus_tag="VNZ06_10885"
     CDS             930..1478
                     /locus_tag="VNZ06_10885"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWW21301.1"
                     /translation="MKRNPLALTGALSVAAAFVATAAGDPSIDASKSTITATFKQENV
                     PVDAAFKSFNGRVDYDPAKPNASKAAIEVATGSFDMGTAEYNAEVKKKAWLDSATYPK
                     ATFISTSVKPGVAGHFDATGALTLKGKSQTLTVPVTATQNGNATVFDGALVISRAYFG
                     IGDAEWSDVVDDKVRIRFHLVE"
     gene            1616..1840
                     /locus_tag="VNZ06_10890"
     CDS             1616..1840
                     /locus_tag="VNZ06_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004644972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWW21302.1"
                     /translation="MYVCICHGITDRQICRAVDQGAHSLDEVQMQLPVGGCCGRCVDT
                     ARAIIRDHQHSPGRCAVRPSARVAEEPALA"
     gene            1948..2424
                     /gene="bfr"
                     /locus_tag="VNZ06_10895"
     CDS             1948..2424
                     /gene="bfr"
                     /locus_tag="VNZ06_10895"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HWW21303.1"
                     /translation="MKGDARILKHLNTVLRNELTSINQYFLHSRMYGNWGLQRLRQHE
                     YEESIDEMKHADRLIERILFLEGLPNLQDLGKLLIGEQVPEIIECDLKLERAAHADLK
                     IAIVDCEQVGDYVSRELFEKILESEEEHIDWLETQLDLIRRVGLENYTQSQMQSPS"
     gene            2461..2799
                     /gene="glnK"
                     /locus_tag="VNZ06_10900"
     CDS             2461..2799
                     /gene="glnK"
                     /locus_tag="VNZ06_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWW21304.1"
                     /translation="MKLITAIIKPFKLDEVRAALSELGVSGMTVTEVKGFGRQRGHTE
                     LYRGAEYVVDFVPKARLDIAVRSDLVEQVVDAIVRTAKTGKVGDGKIFISELQRVIRI
                     RTGETDNSAL"
     gene            complement(2827..3441)
                     /locus_tag="VNZ06_10905"
     CDS             complement(2827..3441)
                     /locus_tag="VNZ06_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21305.1"
                     /translation="MTSSIAPSDPHLTGQWRLDKSASDDADAKISVAVDAAESKLRRR
                     LANAGYSQYEQPAGGRRHGGNAGGGGASSNGAGLNGEEFSQTGYIGPDFNALRRNLQR
                     VLASPTTLRIDVKPDDVRIAGDDNPPRDYPPDDEFTRIDEYGTARIDTSWSGTTFNLK
                     LRYESHATVRESYSTDMHTDTLTITRTLSDPVAGKISVRSLYRH"
     gene            3619..4215
                     /locus_tag="VNZ06_10910"
     CDS             3619..4215
                     /locus_tag="VNZ06_10910"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HWW21306.1"
                     /translation="MYRVALYLIAGWSGLFVMGIELLGGRVLAPYFGSSIYVWGALIT
                     VFMLALSLGYLCGGRASMHSPSLRRLSVILVGAALATLPVLLLGEPLLDYLSSVLTDP
                     RFGSLCAASLLFFAPTLISGMVSPYAIRLLVADQASAGQRAGLLYFASTFGSAAGTIA
                     TSFYLVLWLEVNQILLLLMGISVLVSLGAWLLSARNDE"
     gene            4212..5132
                     /locus_tag="VNZ06_10915"
     CDS             4212..5132
                     /locus_tag="VNZ06_10915"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HWW21307.1"
                     /translation="MSANQDQRCFTGWFAALMCCFVLPAATQVPDQARLIHSEKSLYR
                     QVLVYEDDVNRCLCFTHQCAIGRQSCIDLKLPNHLVFEYTQMMLGSLFLVPDPQHVLV
                     IGLGGGTLPLTLEKLVPHADIDTVEVDPAVVIVAQRYFGFHADPRLHVIELDGRAYVR
                     QAIRAGKRYDLIMLDAYDNQYIPEHLLTREFLSEVRQLLRPGGIVAANTFSQSKLYSN
                     ESVTYRAVFGQFYNVKSGNRVILASNGPLPSIDTVTHNSRQFTNSFDALGISSKRLVG
                     QFSVEADWDTGARVLTDQYSPANLLNSAVP"
     gene            complement(5162..5929)
                     /locus_tag="VNZ06_10920"
     CDS             complement(5162..5929)
                     /locus_tag="VNZ06_10920"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HWW21308.1"
                     /translation="MQKHIWWVGAALVLIAAVVLFFSWRANRHAPPPAATAPAPSAPA
                     PAIQNPVPAAPTPQPLPPLQSSDGPLHEAMSELMGKRATDSLLKPEQLIRHIVVTVDN
                     LSRKKAAFESRPTKPLPGSFMAKGDEQHSILDPSNYQRYTAIMQVVQMVDSKQLAALY
                     FHYYPLFQQEYQNQGYPNGYFNDRLVATIDNLLATPDVSGDIVLVRPNVMYQFADPNL
                     EDLSAGQKALLRVGPQNAAIIKAKLRELRTQIASAPH"
     gene            6053..>6657
                     /locus_tag="VNZ06_10925"
     CDS             6053..>6657
                     /locus_tag="VNZ06_10925"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HWW21309.1"
                     /translation="MVRPARFAHNNQTAGSNRFQQSAAQSDRTAAMAAAEFDALSSGI
                     AMTGVAVCCVDDTPEPPKPDAAFPNNWVSFHRDGTVVMYPMLAPNRRAERRPEILSIV
                     EQRLQFRRRRLIDLSVHEREGRFLEGTGSLVLDHTQRIAYACRSGRTDEALVRAWSQQ
                     LGFEPLLFDAAGPDRTAIYHTNVLLSVGTAWAVVCTDTIAEG"
BASE COUNT         1250 a         1954 c         2057 g         1396 t
ORIGIN      
        1 gagtcgtagg ttacagtggg ccgcagcgcc cgcgacagaa gttctttccg cccgagtggg
       61 cggtggtacg catgctgtcg gtcctgccgg ccgaacgcgg gggaggactt gggcggctgc
      121 tcacgcaggc atgcatcgat cgcgcgcgcc gcgaccgggt cgccaccatt ggcctgcaca
      181 ccagtccggc catgccggcg gcactggggc tgtatctgcg catgggattc gccctttgcc
      241 agaccctgcc agagatgaga ggtgtgccgt acgccctcta cacccggcac ctgacggatg
      301 actagcaacc ggtctttgac cggccctggc ggagttacta acatggctac acaacacgaa
      361 aggactgaag tgcaggcact cgctcgttac actcgaacgg ccatctcgct gcactgggtg
      421 atgtttctgc tcatggcggc gggcttcacc atcggtgctt atatggccga catgcacgtc
      481 agtcctctca aggcgcgact gttttcgtat cacaagtgga tcggcgtcac ggtgctcggc
      541 tttgcgctga tcagggtgct gtggcgtctg acacaccagc caccgcccaa tctgccgatg
      601 ccacgctggc agcagatcgc cgcccacagc gtccattggt tgctgtacgc gctgatgatc
      661 gccacaccca tggtcggctg gctctatagc tcggccgccg ggtttccgat cgtctatctg
      721 aagctttggc agctgcccga tctggtgcac aagaacgagg cactggcgaa agtactggtg
      781 caggtgcacg gttttctcgc ctggacgtta ttgtgggtcg ttgcgttaca cgccctcgcc
      841 gcgctcaaac atcatttctt cgaccgagac gcgaccctga aacgcatgtg gagctgacaa
      901 cgcccccaaa ccaacctgag gagctgtgaa tgaaacgcaa tccactcgcc ctgaccgggg
      961 cactgtccgt ggcggcggcg ttcgtggcaa ccgcggccgg cgatcccagc atcgacgcgt
     1021 ccaagagcac catcacggcg acgttcaagc aggagaatgt gccggtggat gccgccttca
     1081 agagtttcaa tggccgcgta gactatgatc cggcgaagcc gaatgcttcc aaagcggcca
     1141 tcgaggtcgc taccggcagc tttgacatgg ggaccgcgga gtacaacgcc gaggtcaaga
     1201 agaaggcctg gcttgacagt gccacttatc cgaaggcgac cttcatttcc acctccgtca
     1261 aaccgggcgt ggccgggcat ttcgatgcca ccggagcact gaccctcaag gggaagagcc
     1321 agaccctgac cgtacccgtc accgccaccc agaatggcaa tgcgaccgtc ttcgacggcg
     1381 cattggtaat ctccagagcc tactttggca ttggcgacgc cgaatggagt gacgtcgttg
     1441 atgataaggt gcgcatcaga tttcacttgg tagaataata acttagctca aatcttggtc
     1501 tggggatccg ggctggatta cgtccaatta gtattgacaa tcattctcat tcacattacc
     1561 gttgaggcgt tgtcacagcc accggtctgc cccggtgaag gttcgaagcg tcaccatgta
     1621 cgtctgcatc tgccacggga tcaccgatcg tcagatctgc cgcgcggtcg atcagggcgc
     1681 gcattccttg gacgaagttc agatgcagtt gccggtgggt ggctgctgcg gccgatgcgt
     1741 cgataccgcg cgtgccatca ttcgcgatca ccagcattcg cccggccgct gcgctgtccg
     1801 tccgtcggct cgggtcgccg aagaacccgc gctcgcctaa gaatcacacc ccgaagcggc
     1861 gtgcatcgcg cgtgattgca tcacggccgc ggcgcactca atatcaaacc tgcctcaccg
     1921 atcggtacaa gaccggagcc atgcgctatg aagggcgacg ccaggatcct caagcatctg
     1981 aataccgttc tgaggaacga actgacctct atcaatcaat acttcctgca ttcgcgcatg
     2041 tatgggaact ggggtctgca gcgccttcgt cagcatgagt acgaggaatc gatcgatgag
     2101 atgaagcatg ccgaccgtct gatcgagcgg attctgtttc tcgaggggct gccgaacctg
     2161 caggatctgg ggaaactgtt gatcggtgag caggtgccgg aaatcatcga gtgcgatctg
     2221 aagctcgaac gcgcggcgca tgcggacctg aagattgcca tcgtggactg cgagcaggtg
     2281 ggcgactacg tctcgcgcga gctgttcgag aaaattctcg aatccgagga agaacatatc
     2341 gactggctcg aaacgcagct cgatctgatt cggcgcgtgg gtctggagaa ctacacgcaa
     2401 tcgcaaatgc aaagcccaag ctgataccat tcatcgtcgg ccgtcaggcc ggaggctgcc
     2461 atgaaactca tcaccgccat catcaagcca ttcaagctcg acgaagtgcg cgccgcgctg
     2521 tccgaattgg gcgtatcagg aatgacggtg acggaagtga agggcttcgg acgccaacgc
     2581 gggcacaccg agctgtatcg gggcgcggaa tacgtcgtgg atttcgtgcc caaggcgcga
     2641 ctggatatcg cggtgcgttc cgacctggtg gaacaggtgg tggacgcgat cgtgcggacc
     2701 gcgaagaccg gcaaggtggg cgacggcaag atcttcatca gtgagctgca gcgggtgatc
     2761 cggatccgca ccggcgaaac cgacaactcc gctctgtgat gtccttcagg ctcccaggag
     2821 ccgtcctcag tgacgataca gcgaccgcac gctgatcttg ccagccaccg ggtcggacag
     2881 ggtgcgcgtg atcgtcaaag tatcggtatg catgtcggtg ctatagcttt cgcgcaccgt
     2941 cgcatggctc tcgtagcgca gttttaagtt aaaggtcgtt ccggaccagc tggtgtcgat
     3001 acgcgcggta ccgtattcat cgatccgcgt aaattcatcg tccggcggat agtcgcgcgg
     3061 tggattgtcg tcgccggcga tgcgcacgtc atccggcttc acgtcgatcc tgagcgtggt
     3121 tggcgaggcg agcacccgct gcaggttgcg ccggagtgca ttgaaatcgg ggccgatgta
     3181 gccggtctgg ctgaactcct ctccgttgag tccggcaccg ttgctggagg cgccaccgcc
     3241 accggcattg ccgccgtggc gccgtccgcc ggccggttgc tcgtattgtg agtatccagc
     3301 gttggccagg cgcctgcgca gtttggattc ggccgcatcc accgcgactg agatcttggc
     3361 gtcggcatcg tcactcgccg acttatccag ccgccattgc ccggtcagat gcgggtccga
     3421 cggcgcgatt gaactcgtca cacaggaggc agcgcccagc gttaaggcaa ctgcgacagt
     3481 cagcggagca atggctttga acatcgaagt tcagtaggac cgtctaaagc gccgatggtt
     3541 cggtcagcgc ggggcacagg ctagagaggt gcggtaaccc tgtcaaaccg ggtatcggcg
     3601 cgcgctacac tgatagggat gtatcgcgtt gcgctgtatc tgattgccgg ctggtccggt
     3661 ctcttcgtga tgggtattga actgctcggc gggcgcgtac tggcgccgta cttcggcagc
     3721 agtatctacg tctggggcgc gctgatcacc gtgttcatgt tggccctgtc gctggggtat
     3781 ctgtgcggtg gccgcgcctc gatgcattcc ccgagtctgc ggcgactgtc ggtgatactg
     3841 gtgggagccg cgctggcgac gctgccggtg ttactgctcg gcgagccgct gctggactat
     3901 ctgtcgtcgg tattgaccga tcctcgattc ggttcactct gcgccgccag cctgctgttc
     3961 tttgcgccca ccctcatttc agggatggtc tcgccctacg cgattcgctt gctggtggcc
     4021 gatcaggcca gcgccgggca gcgcgcgggg ttgctgtatt tcgcttccac gtttggcagt
     4081 gctgccggca cgattgcgac gtcgttttat ctggtgctgt ggctcgaagt gaaccagatt
     4141 ctgctgctgc tgatgggaat ctccgtgctg gtgtcgctcg gggcctggtt gctttcggcc
     4201 cggaatgacg agtgagcgcg aatcaggatc aacgctgttt cacagggtgg tttgcggcgc
     4261 tgatgtgctg cttcgtgctg ccggccgcta cccaggttcc ggatcaagcg cggctgattc
     4321 attccgaaaa atcgctctat cgacaggtgc tggtctatga ggatgacgtc aatcgctgcc
     4381 tgtgttttac ccatcagtgt gcgattggac ggcagtcgtg catcgatctt aagctgccga
     4441 atcacctggt gttcgagtac acccagatga tgcttgggtc actgtttctg gtccccgacc
     4501 cgcaacacgt tctggtgatt ggcttagggg gagggacctt gccgctgaca cttgaaaaac
     4561 tagtgccaca cgctgacatc gatacggtgg aagtcgatcc cgcggtggtg atcgtcgctc
     4621 agcgttactt tggctttcat gccgacccgc gactgcacgt gatcgagctg gatggtcgtg
     4681 cttatgtcag acaggcgatt cgtgccggca aacgctatga cctcatcatg ctggacgcct
     4741 acgacaacca atacattccg gagcacctgc tgacacgcga attcctgagc gaggtgcggc
     4801 aactgctgcg acctggcggc atcgtggcgg cgaatacttt ttcgcagagt aaactctatt
     4861 cgaacgagtc agtgacttat cgcgccgtct ttggccagtt ctacaacgtc aagagtggca
     4921 accgtgtgat tctcgcgagc aacggtccgc tgccttcgat cgacacggtt acgcacaaca
     4981 gccggcaatt caccaactca ttcgacgcac tggggatctc cagcaagcgg cttgtggggc
     5041 aattctcggt tgaggctgat tgggacacgg gcgcccgcgt cctgactgac caatattccc
     5101 cggctaacct gctgaacagc gcggtgccct agagcatacg ttgccggcag atgcttcgcg
     5161 gctagtgcgg agcactggca atctgcgtgc gcaactcgcg cagcttggct ttgatgatgg
     5221 cggcattctg tggacccacg cgcaacaacg ccttctgtcc cgccgagaga tcctcgagat
     5281 ttggatcggc aaactgatac atcacattcg ggcgcaccag cacaatatcc ccgctgacat
     5341 cgggtgttgc cagcaggttg tcgatcgtag ccaccagacg gtcattgaaa tagccgttcg
     5401 gatagccctg attctggtac tcctgctgaa atagcgggta gtagtggaag tagagtgccg
     5461 ccagctgctt gctatcgacc atctgcacta cctgcatgat ggccgtgtag cgttggtagt
     5521 tggaaggatc gagtatcgaa tgctgctcgt cgcccttggc catgaagcta cccgggagcg
     5581 gtttggtcgg tctcgattca aacgccgctt tcttacgcga caggttgtcc accgtcacca
     5641 cgatgtgccg tatcaactgt tcgggcttta agaggctgtc ggtcgcacgc ttgcccatca
     5701 gctcggacat cgcctcgtgc agcggcccgt cgctggattg caggggtggc agcggctgtg
     5761 gtgtgggggc tgcgggaacc ggattctgaa tcgccggggc cggggcgctt ggcgcgggcg
     5821 cagtcgcggc agggggaggg gcgtgccgat ttgcgcgcca ggaaaagaac agcactacgg
     5881 cggcgatcag gaccagggct gcgccgaccc accagatgtg tttttgcatg attggaaacc
     5941 tagtctcctt gggatgcgat tgcaaccgca ctgcagcgct gctctcacgt attatctgcc
     6001 tgtgaatgca gtatccgagg gcctctctga acagagcgcg aacggcgtgc tgatggtgcg
     6061 accggcaagg ttcgcgcaca acaatcagac cgcgggcagc aaccgcttcc agcagtcggc
     6121 tgcgcagtcg gatcgcacgg cggctatggc cgcggccgag tttgacgcac tctccagtgg
     6181 aattgcgatg accggggtcg cggtgtgctg cgtggatgac acccccgaac cacccaagcc
     6241 ggacgcggca ttcccgaaca actgggtgag cttccaccgt gatggtaccg tggtgatgta
     6301 tccaatgctg gcacccaacc gtcgcgctga acgacggccg gagattcttt cgatcgtcga
     6361 acaacgactg caatttcggc gtcggcgcct gattgatctc agtgtccacg agcgcgaggg
     6421 ccgcttcctc gaaggcacag gtagtctggt gctggatcac acccagcgca tcgcctacgc
     6481 ctgtcgctca ggccgtaccg atgaggccct ggtgcgcgcc tggtcacagc aactgggttt
     6541 cgagccgctg ctatttgacg ctgccggtcc cgacaggact gcgatctatc acaccaatgt
     6601 attgttgagc gtcggcacag cctgggcggt ggtgtgcacc gataccatcg cagaggg
//
LOCUS       DATMRM010000362         3442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2989708, whole genome shotgun sequence.
ACCESSION   DATMRM010000362 DATMRM010000000
VERSION     DATMRM010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2989708"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VNZ06_10930"
     CDS             complement(<1..856)
                     /locus_tag="VNZ06_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWW21310.1"
                     /translation="MDTTTAANGSPPRLALRGITKRYASVTANERIDLSVAGGEIHAL
                     LGENGAGKSTLMKIVYGVTQPDAGSIEWQGEVVAIHSPAHARRLGIAMVFQHFALFET
                     MTVAENIALATDERLPPAALAVRIEEVSTRYGLPVDPQRLVHSMSVGERQRVEIVRCL
                     LQSPKLLILDEPTSVLTPQAVQALFQTLRQLAREGLSILYISHKLDEIRELCDVATVL
                     RNGRVSGAAVPREQSNAGLARMMMGSELKQCRLEPHPTGEVQLELVGLSLPSADPFGT
                     SLRRLDLSV"
     gene            complement(867..1913)
                     /locus_tag="VNZ06_10935"
     CDS             complement(867..1913)
                     /locus_tag="VNZ06_10935"
                     /inference="COORDINATES: protein motif:HMM:NF018249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside permease"
                     /protein_id="HWW21311.1"
                     /translation="MTRRALSWLLPAVLMAGAAVRGSATAPAAAELVPVKVMVVSMFG
                     LEAAPWLKALKITREIAVPGLSSDFPLVRCSADGVCQMTSSMGHANAAASIMAVAYSG
                     RFDLRRTYFLIAGIAGIDPDRGTIGSATWARFAVDAGIAHEVDVRDLPKDWNDGYYGV
                     LTNSPDQKPRFDYHTEVFRLNEALLQRALALSKDVQLADSDDVRAYRAHYPKAPANQP
                     PQVIQCDTASGDTWWAGPHLEEHARHWVKLLTDNQGVYCTTQQEDNATMIALTRASQS
                     GLVDLRRVAILRTASDFDRPYPGQSTVDGLHAQLAIAGAGTISTDNLVKAGMPLVEAI
                     VANWPQWQAGVPAN"
     gene            1993..3072
                     /locus_tag="VNZ06_10940"
     CDS             1993..3072
                     /locus_tag="VNZ06_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005251735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HWW21312.1"
                     /translation="MSHRLRPMLTTACLVALSAICSAASAPLKVAFVYVGPISDGGWS
                     YQHHLGKLALDKEFGPRVQSSYVESVPETADAERVIRQLAAKGNDIIFTTSFGYMDPT
                     LKVAKLFPKVKFEHASGYKTAPNMVAYEARFYEGAYLLGVLAGRTTRTNTLGFVGSFP
                     ISEVIRNIDAFTLGARSVNPKIHTKVVWVDTWYDPGKERQAAEALIAQGADVLCQNTD
                     SPATVQVAEEKGLHAFGWDSDMAKYGPHAQLTANTENWGVYYIDEIRKVFDGSWSGDR
                     KIKWGIKEGAVALTPLNPSVAADVAKLFESKKQAIAAGTLIPFAGPLKDNTGAPKVAA
                     GSAMTVDELVSINWYVEGVDGTVPK"
     gene            3119..>3442
                     /locus_tag="VNZ06_10945"
     CDS             3119..>3442
                     /locus_tag="VNZ06_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21313.1"
                     /translation="MKILVTGSGAQDATEDAARRTAVAYASQLRIWALVMCVVLAVTW
                     VLPISYAAVIAGHAPATAAARLWRGYWWLGAAPALAAALLPPGWFRPGAWERGGDIYK
                     PFGDGR"
BASE COUNT          631 a         1099 c         1103 g          609 t
ORIGIN      
        1 gtacgctcag atccagccgc cgcagcgaag tgccaaaagg atccgcactc ggcagcgaca
       61 ggccgaccag ttcgagctgc acctcgccgg tcggatgcgg ctccagccgg cactgcttca
      121 gctcactgcc catcatcatc cgcgcgagac cggcgttgct ctgctcacga ggaacggcgg
      181 cgccgctgac ccggccgtta cgcaacaccg tcgcgacatc gcacagctcg cggatctcat
      241 cgagcttgtg gctgatataa agaatgctca ggccctcccg ggcaagctgc cgtagcgtct
      301 ggaacagggc ctgcaccgcc tgcggtgtca gtaccgaggt cggctcatcg aggatcaata
      361 acttcgggga ctgcagcagg caacgcacga tctccacgcg ctgacgctcg cccaccgaca
      421 tgctgtgcac cagccgctgc ggatcgaccg gcagaccata gcgcgtggac acttcctcga
      481 tgcgcaccgc cagcgccgcc ggtggcaggc gctcatcggt cgcgagcgcg atgttctcgg
      541 ccaccgtcat cgtctcgaac agggcgaagt gctgaaacac catcgcgatg ccgagccgcc
      601 gggcgtgcgc cggcgaatga atcgccacca cctcgccctg ccattcaatg ctgccggcgt
      661 caggctgcgt cacaccgtag acgattttca tcagcgtgct cttgccagcg ccgttctcac
      721 ccaacagcgc atgaatctcg ccgccagcga cactcagatc gatgcgctcg ttggctgtga
      781 cgctggcgta tcgcttggtg atgccgcgca gtgccagacg cggtggcgag ccgttcgccg
      841 ctgtggtggt atccatcgca agtccttcag ttcgcaggta cgcccgcctg ccattgcggc
      901 cagttcgcga caatcgcctc caccagcggc atgccggcct tcaccagatt gtcggtcgaa
      961 atggtgcccg ccccggcgat cgcgagctgt gcgtgcagtc catccaccgt actctgcccc
     1021 gggtagggcc ggtcaaagtc cgaggcggtg cgcagtatcg ctacgcgccg cagatccacc
     1081 agtccggact gcgaagcgcg ggtgagcgcg atcatcgtcg cgttatcctc ctgctgcgtc
     1141 gtgcagtaga caccctgatt gtcggtcagc agcttcaccc agtggcgggc gtgttcctcc
     1201 agatgcggcc cggcccacca ggtatcgccc gaggcggtat cgcattgaat cacttgcggc
     1261 ggctgattgg ccggtgcctt ggggtagtgc gcccgatagg ctcgcacgtc gtcgctatcg
     1321 gcgagctgaa cgtccttgga aagcgccagc gctctctgca gcagcgcctc gttgagtcgg
     1381 aacacctcgg tgtgatagtc gaacctgggc ttctgatcgg gtgagttcgt cagtacgccg
     1441 tagtagccat cgttccagtc cttgggaagg tcgcgaacat cgacctcgtg cgcaatgcct
     1501 gcgtccacgg cgaaacgcgc ccaggtcgcc gaaccgatcg tgccccggtc cgggtcgatg
     1561 ccggcaatgc ccgcaatcag aaaataggtc ctgcgcagat cgaaccggcc gctataggcc
     1621 accgccatga tcgaggccgc ggcattggca tgacccatgc tgctcgtcat ctggcacaca
     1681 ccgtcggcgg agcatcgaac cagtggaaag tcgctggaca gtccgggcac ggcgatctcg
     1741 cgggtgatct tgagcgcctt cagccacggc gcagcttcca ggccaaacat gctcacgacc
     1801 atcaccttga ccggcacgag ctccgcagcc gcaggagcgg tggcggaacc acggacagcg
     1861 gcacctgcca tcaggacggc aggcaacagc catgacagtg ctcgtcgcgt catgttcgat
     1921 gtcctttgtg gcgcggggtt ggccgatcga tcgtatacaa tcggatcatc actggcgagg
     1981 ctaagaataa tcatgtcgca cagactccgc ccgatgctga cgactgcctg tctggtggca
     2041 ctcagcgcga tctgcagtgc cgcgagcgca cctctgaaag tggctttcgt ctacgtgggt
     2101 ccgatctccg atggcggctg gtcgtatcag catcatcttg gcaagctcgc gctggacaag
     2161 gagttcgggc cgcgggtgca aagcagctat gtcgagagcg tgccggagac cgcggacgct
     2221 gagcgcgtca tccgccagct cgccgccaag ggcaatgaca tcatcttcac gacctcgttc
     2281 ggctacatgg atccgacgct gaaggtcgcg aagcttttcc ccaaggtgaa gttcgagcac
     2341 gccagcggct ataagaccgc gccgaacatg gtcgcctatg aggcgcgctt ctacgagggt
     2401 gcttacctgc tcggcgtgct ggccgggcgc accaccagga ccaatacgct tggattcgtc
     2461 ggctcgtttc cgatctccga ggtgatccgc aacatcgatg ccttcacgct cggcgcgcgc
     2521 agcgtcaatc cgaagatcca caccaaggtg gtgtgggtcg atacctggta tgacccgggc
     2581 aaggagcgcc aggcggccga ggcgttgatc gcccagggcg cggatgtgct gtgccagaac
     2641 accgattctc cggcgacggt gcaggtcgcg gaagagaagg gactacacgc cttcggttgg
     2701 gactcggata tggccaagta tggcccgcac gcacagctga cggccaatac cgagaactgg
     2761 ggtgtctact acatcgatga gatcaggaag gtgttcgatg gcagctggag cggggaccgc
     2821 aagatcaaat ggggtatcaa ggaaggggcc gtggcgctga cgccgctgaa tccttccgtt
     2881 gcagccgacg tcgccaagct gtttgagtcg aaaaagcagg ctatcgccgc cggaacactg
     2941 atcccgtttg caggcccgct gaaagacaac accggcgcac cgaaggttgc cgcgggcagt
     3001 gcaatgacgg tggacgagct ggtgtcgatc aactggtacg tcgaaggtgt ggacggtacg
     3061 gtaccgaagt gaggatcggc gcgtgccgcg caacgttgaa atcaaatctg ctgacggcat
     3121 gaagatcctt gtcaccggca gcggggcgca ggacgcgaca gaagacgccg ccaggaggac
     3181 cgcagtggcc tatgcgtcgc agctgcgcat ctgggctctc gttatgtgcg tggtccttgc
     3241 ggtgacctgg gtgttgccga tcagctatgc ggcggtgatc gcgggccacg cgccggcgac
     3301 ggccgccgcg cgcctttggc gcggctactg gtggctgggc gccgcgcccg cacttgccgc
     3361 tgccctgctg ccgcccggct ggtttcggcc aggggcatgg gagcgtggcg gagacatcta
     3421 caagccgttc ggcgacggac ga
//
LOCUS       DATMRM010000363         4300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1140749, whole genome shotgun sequence.
ACCESSION   DATMRM010000363 DATMRM010000000
VERSION     DATMRM010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140749"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..768)
                     /locus_tag="VNZ06_10950"
     CDS             complement(139..768)
                     /locus_tag="VNZ06_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21314.1"
                     /translation="MLPHSARLFAAGALAAVLCGCATFGFGQRYPATPVSVQVSPDVN
                     PVVAALQLVSELPQKDPAGQAELFQAAKNAAELTPTTSNKLKFALALATPGHSGSDPV
                     AAQRHLAELLATPEDLLPVERYLARSELQQVERQLILDAENRRLRDDAPREARDKLAS
                     VNHRLAAETDENAKLRKELEDARAKLEAVSHIERSINDRNSTATEPSKP"
     gene            complement(755..2185)
                     /locus_tag="VNZ06_10955"
     CDS             complement(755..2185)
                     /locus_tag="VNZ06_10955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW21315.1"
                     /translation="MLIGFTFVAAPLLVAIVNAAVQMSRLSDRSEQLVVHGMQGTRNN
                     QLMFEQISALERTARLYQIIGNADLLDVYARNNDRLMATTRELLRLLPDAESHEQLGG
                     LQAEAQRLHVVLKQTPPASPEMSALINAYPRLLDMASRMSQHSNQQIDRELDDLQIAT
                     DLAQRELFWVTLLLVPVTFLAVGLFTFWFGRPIRAIDRAINELGRGKFSRPIAIRGPR
                     DLEKLAAQLDWLRLRLLELAQEKNRFLRHMSHELKTPLANIREGTELLIDGAVGELQG
                     SQREVVGILRENSMKLQHLIENLLSFSAWQAKSTGLEISEFKLRPLIKGVLENQQLTL
                     IAQRVRLDVKIEDLTPQADRGKLRLILDNLLSNAIKFTPRGGTIAIHALSEREQLIID
                     VIDSGPGIPADERNRIFEAFYQGKTPQGGHVKGTGIGLSVVTEFVGAHGGAIEIIDRP
                     DGGAHFRVRLPLRQTDAAAIRAHAAA"
     gene            2257..3237
                     /locus_tag="VNZ06_10960"
     CDS             2257..3237
                     /locus_tag="VNZ06_10960"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF008738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HWW21316.1"
                     /translation="MKEQTPYASLSPGVVQDALEAVGYLCDGHVLALGSYENRVYQVG
                     VETGEPVVVKFYRPGRWRDEAILEEHAFTLELAAQEIPVVPPSVRDGRSLHRHAGFRY
                     AVFARRGGRWPELGTTDEREWVGRFLGRIHAVGRAGRFLHRPRLSIQELGHKARETVL
                     GGDWMPDYLAAKYADVTQALLAEIEARAPLWGGAAQIRIHGDCHRNNILWTDAGPHFV
                     DLDDCQTGPAIQDLWMLLAGNMQEMRTQLRDLIAGYEKFLPFDRGELALIESLRALRM
                     MHYSAWLARRWHDPAFPQAFPWFATSRYWEDHYRSLDEQLTVLDAPVLEL"
     gene            3267..3803
                     /locus_tag="VNZ06_10965"
     CDS             3267..3803
                     /locus_tag="VNZ06_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21317.1"
                     /translation="MRRRSSPQSGAANARLGMCGAMLVLAACGGSKKVDTADQLAAKN
                     AAAAAKQHPSTSAEQTATMVQAPTSGKATAPMQLKFELAERPVAGQTVDMGLALMVTV
                     PAQSVTLHLADAPGIVYADAADRLLGAVVPDTVYRQDIKLSATAEGVYFVGVTAIMSR
                     DTLTETRNFSIPIIVSAE"
     gene            3864..>4300
                     /locus_tag="VNZ06_10970"
     CDS             3864..>4300
                     /locus_tag="VNZ06_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWW21318.1"
                     /translation="MTQQLRNIAIIAHVDHGKTTLVDQLLRQSGTLDARKDLQERVMD
                     SNVLERERGITILAKNTAINWGDYRINIVDTPGHADFGGEVERVLAMVDCVLLLVDAV
                     DGPMPQTRFVTQKAFKHGLHPIVVINKIDRDEARPGWVLDQTF"
BASE COUNT          797 a         1331 c         1355 g          817 t
ORIGIN      
        1 atcgacgacc aggatgcggg ccttgcgcgg ctggggcgtc gacgcctcgc gcttgccagc
       61 gggttgctgc aggccaaaat cgagcggaac cgtcgcggcg gtggcgtgcg gagtcacggt
      121 gtcgtctccc gtcggcattc agggcttgga aggttcggtg gcagtcgaat tacgatcatt
      181 gattgagcgc tcaatatggg aaactgcctc aagtttagcc cttgcgtcct ccagttcctt
      241 gcgcagcttc gcattttcgt cagtctccgc cgctaatctg tgatttacgc tggcaagctt
      301 gtcacgggcc tcacgcggtg catcatccct aaggcgcctg ttttcggcgt ccaggatgag
      361 ctgccgctcg acctgctgca gctcgctacg cgccagatag cgctcaacgg gcagcaaatc
      421 ctcaggcgtt gcaaggagtt ccgccaggtg tcgctgcgcg gcgacgggat cagatccgct
      481 gtgccccggg gttgcgagcg cgagtgcgaa cttcagtttg ttgctggtgg tcggcgtgag
      541 ctccgcggca ttcttggccg cctgaaacag ttcggcctgt ccggccgggt ctttctgggg
      601 caattcactg accagctgca aggcggcaac gactggattg acatcgggcg aaacctgcac
      661 tgaaaccggc gtcgcagggt agcgctggcc aaacccgaac gtggcgcagc cgcacagcac
      721 agcggcgagc gcgccagcgg cgaagagccg cgcgctatgc ggcagcatgc gcccgtatcg
      781 cggcagcgtc tgtctggcga agcggcaagc ggacccgaaa atgcgcgcca ccatccgggc
      841 ggtcaatgat ctcgatcgcg ccgccgtgcg cgccgacgaa ttccgtgacc acggagagcc
      901 caatacccgt tcctttgaca tgcccgccct gaggagtctt gccctggtag aaagcctcaa
      961 aaatccggtt gcgctcgtcg gccggtatcc caggcccgct gtcgatcacg tcgataatca
     1021 gctgttcgcg ttcgctcagc gcatgtatcg cgatggtacc gccacgtggc gtgaacttga
     1081 tggcattgga caataggtta tcaaggatca ggcgtaattt cccgcggtct gcctggggcg
     1141 tcaggtcctc gatcttcaca tcgaggcgca cgcgctgcgc gatcagcgtc agctgctggt
     1201 tctccagcac acctttgatc agcggacgca atttgaattc gctgatctcg agccccgtgc
     1261 tcttggcctg ccaggcactg aaggagagga gattctcaat caggtgctgc aacttcatgc
     1321 tgttctcgcg cagtatcccg acaacctcac gttggctgcc ctgaagttct cccaccgcgc
     1381 catcgatcag caattcggtg ccctcgcgaa tgtttgcgag cggcgtcttc agttcgtgcg
     1441 acatatgccg caggaatcga ttcttctcct gggccagttc gagcagccgc aggcgcagcc
     1501 agtcgagctg cgcggcgagc ttttccagat cgcgcggtcc gcgtatcgca atggggcggg
     1561 agaacttgcc tcgccccagt tcattgatgg cgcggtcgat cgcacggatg ggccggccga
     1621 accagaacgt gaacaggccg acggccagga atgtcacggg caccagcagc agggtcaccc
     1681 agaacaactc gcgctgcgca aggtccgtcg ctatctgcag atcgtcgagc tcgcgatcga
     1741 tttgctgatt gctgtgctgg gacatccgcg atgccatatc aaggaggcgc ggataggcat
     1801 tgatcagggc ggacatctcg ggcgacgcgg gtggcgtctg cttaagtacc acgtgcaggc
     1861 gctgcgcctc ggcctgcagg ccccccagct gctcatgcga ctcggcgtcc ggcagcaggc
     1921 gcagcaattc acgcgtggtc gccatcaggc ggtcgttgtt gcgtgcatac acatccaata
     1981 gatcggcgtt gcctatgatc tggtagagcc tcgccgtgcg ctcgagcgcg ctgatctgct
     2041 cgaacatcag ctggttattg cgcgtccctt gcataccgtg cacgaccagc tgctcactgc
     2101 gatccgacag gcggctcatc tgcaccgcgg cattgacgat cgcaaccagc agcggcgcgg
     2161 ccacgaacgt gaagccgatc agcatgagcc cgcttaatga cctgggacgc aacaagcgca
     2221 tgctggccat tctagcagcg ctgtagactc tcatatatga aggaacagac cccctacgcg
     2281 tcgctctcgc ccggcgtcgt tcaggacgca cttgaggcgg tcggctacct gtgcgatggg
     2341 cacgtgctcg cccttggcag ttacgagaat cgcgtctacc aggtcggcgt cgaaaccggc
     2401 gaaccggtcg tcgtcaagtt ctatcgtccc gggcgctggc gcgacgaggc gatcctggaa
     2461 gaacacgcgt tcacgctgga gctggccgca caggagattc cggtcgtacc cccgagcgtg
     2521 cgggacgggc gctcgctgca caggcacgcc ggctttcgct atgcggtgtt cgcgcgccgt
     2581 ggcgggcgct ggccggagct tgggaccacg gacgagcgcg aatgggtcgg ccgcttcctc
     2641 ggccgcatcc acgcggtggg gcgtgcagga cgattcctgc accggccaag actcagtatc
     2701 caggaactcg ggcacaaggc gcgcgagacc gtgcttggcg gcgactggat gccggactat
     2761 ctggccgcga agtacgccga tgtgacgcag gcgttgctcg ccgagatcga ggcccgggcg
     2821 ccgctgtggg gcggtgcggc gcaaattcgc atccatggag attgccatcg caacaacatc
     2881 ctgtggactg acgcaggtcc gcattttgtc gatctcgacg attgccagac cggacctgcg
     2941 attcaggatc tgtggatgct gctcgcagga aacatgcagg agatgcgcac gcagttgcgc
     3001 gacctgatcg caggctatga gaagtttctg ccgtttgacc gcggcgaact cgcgctgatc
     3061 gagtcgctgc gggccctgcg catgatgcat tattcggcat ggctcgcgcg ccgctggcat
     3121 gacccggcgt ttccgcaggc ttttccatgg tttgcgacgt cacgatactg ggaagatcac
     3181 tatcgctcgc tggacgaaca attgacagtc cttgacgcgc cggtgctgga gttgtgaaca
     3241 acccagattt tgaggatcat agtcccatga gacggcgatc tagcccccag agtggcgcgg
     3301 caaacgcccg gcttggcatg tgcggcgcga tgctcgtact ggctgcgtgt ggcggcagta
     3361 aaaaagtgga cactgcggat cagctggccg cgaagaatgc cgcggcggcg gccaagcaac
     3421 atccttccac ctccgccgag caaactgcga ccatggtcca ggcccccacc agcggcaaag
     3481 ccaccgcgcc gatgcaactg aaattcgagc ttgctgagcg accggtcgcc ggacagactg
     3541 ttgatatggg tcttgcgctg atggtcacgg tgccggccca atcggtgacg ctgcacctgg
     3601 ccgacgctcc gggtatcgtg tacgccgatg cggcggaccg gctgctcggc gcggtggtgc
     3661 ccgacacggt ctatcgccag gatatcaagc tgagcgctac ggccgagggc gtctatttcg
     3721 tcggcgttac ggcaataatg tcacgcgata cgctgaccga gacacgcaac ttcagcatcc
     3781 cgatcatcgt cagcgccgaa taaaacaaac tccccgcaag ggcgctgaag tcactataat
     3841 gatcggtttc ctggaacctc atcgtgaccc agcaacttcg caacatcgcc atcatcgcgc
     3901 acgtcgatca tggcaagacg actctggtcg atcagctgct gcgccagtcg ggcacgctcg
     3961 acgcgcgcaa ggatctgcag gaacgggtca tggactccaa tgtgctcgag cgcgagcgtg
     4021 gcatcacgat cctggcgaag aacacggcga tcaactgggg cgactaccgg atcaatatcg
     4081 tcgatacgcc gggccacgcc gatttcggcg gcgaggtcga gcgcgtgctt gccatggtcg
     4141 attgcgtgct gctgctcgtc gatgccgtcg acggaccgat gccgcagaca cggtttgtca
     4201 cacagaaggc attcaagcac gggctgcatc cgatcgtcgt gatcaacaaa atcgatcgcg
     4261 atgaggcgcg ccccggctgg gtcctcgatc agaccttcga
//
LOCUS       DATMRM010000364         7330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4568959, whole genome shotgun sequence.
ACCESSION   DATMRM010000364 DATMRM010000000
VERSION     DATMRM010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4568959"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="VNZ06_10975"
     CDS             complement(<1..1467)
                     /locus_tag="VNZ06_10975"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWW21319.1"
                     /translation="MVVTGSLIPQTDVSIQTPVTVISNEDIQARGFADIAEALQRSSF
                     STGSIQNGQFAGFTQAAKVTSLFGLDVSFTKYLIDGRPIADYPALYNGSENFVSISGI
                     PTVLVDHIDTLPGAQSSIYGSDAIAGVVNVVMKKNLDGPVVDARYGWTADGGGNDRRF
                     AIGDGFTIGNFNVVIGGQYENLSPIWGYQRPLTSQYFTQGTTPQTAERDYLVLGLFGQ
                     PNGDLYYFPDPNNCANTTEGFGGSERLYTRQNRGSYCGTTRSGFYTVANGDESTQGYL
                     HATYDVTDTLQIFVESLVNHDVSKFNVGQLFYGSSVDATSPFYYYEDPNLDAINVNNG
                     GAPDYYNVQHIFSPEESGGRDKNDDKDTNDSIRATLGVQGDFLSSWKYIADFTYTRNK
                     LTERTSLLFAPQVESFFANIFGPQLGVGPLGDFEYTPNYAKFYTALTPAQYDSFFGQI
                     NSYSQTEESFARAQLTTTDLFPLPGGNAGLAVELDGGDQ"
     gene            complement(1869..2099)
                     /locus_tag="VNZ06_10980"
     CDS             complement(1869..2099)
                     /locus_tag="VNZ06_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21320.1"
                     /translation="MWQQLLIGLVVLAAAGYVTWSFLSMPTRQRVLDALAKRGLLIEV
                     SRRHRARLSAPGCSNCAASPQQPRLARSEKQP"
     gene            complement(2109..3968)
                     /gene="feoB"
                     /locus_tag="VNZ06_10985"
     CDS             complement(2109..3968)
                     /gene="feoB"
                     /locus_tag="VNZ06_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HWW21321.1"
                     /translation="MQVRRVALVGAPNCGKTALFNRLTGSRQKVANYPGVTVERKEGA
                     FVGAQPQWLYRVMDLPGTYSLVPTSLDEAITRDAVLGRLNGETPPDLIVCVLDATNLR
                     LSLRLALELARLSIPMVVALNMSDLAHRRGFMLDAPALSRELGVPVVETVAVKSGGEA
                     NLIAALDQVVVGPARQAFTLPGSSHEELAATQREARRILSAVGYVEPLRDRALARIDA
                     VVMNPVLGPILLAALLFLVFQAVFSWAKSPVNWINAGITALGDAVQALLPEGPLRSLI
                     VGGVIAGAGSVLVFLPQILILFFFILLLEDSGYLPRAAFLLDRLMGGVGLSGRSFIPL
                     LSSFSCAIPGIMATRTIPNLRDRLATILLAPMMTCSARLPVYALIIGAFIPARSIGIF
                     NLQGLVLFSLYVLGVAGALLVALVMKRATLRSNYHPLLMELPEYHWPTVRNLLTGLWE
                     RTQIFLTRVGTIILALMVLLWFLASYPAPPSGADGPAIRYSFAGMLGAGLQHLFAPLG
                     FNWQISVALVPGIAAREVVVGALGTVYSLSGNGGADVERALKPMIAGSWSLATALSLL
                     AWYVFAPQCLSTLVTVRRETNSWRYPLIMAAYLFLLAYIAALVTYRVALALGA"
     gene            complement(4020..4322)
                     /locus_tag="VNZ06_10990"
     CDS             complement(4020..4322)
                     /locus_tag="VNZ06_10990"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HWW21322.1"
                     /translation="MDSCRSWHQTDAIQPLAQLRKGCRGIVSAISDLDAGHSLGDAVG
                     STVARRLMELGFIAGERIEVIEEVRPGGDPIAVRIGCSMFALRRREAQAVLVQVEA"
     gene            complement(4461..4940)
                     /locus_tag="VNZ06_10995"
     CDS             complement(4461..4940)
                     /locus_tag="VNZ06_10995"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWW21323.1"
                     /translation="MNTTSHGAAASQSGLTGLIERILNWLDRVPYSLLAIPARLAVAE
                     VFWSSFSTKIQNWDTTVALFEDEYKLPLLSPDAAAHLTAGIEATSSILLVLGLLTRPV
                     AFLLLGMSAYIEAFIYPRAWPTHIQWAAMLVFLLCRGPGKVSLDYLIKRWLGIGEAI"
     gene            complement(4937..5722)
                     /locus_tag="VNZ06_11000"
     CDS             complement(4937..5722)
                     /locus_tag="VNZ06_11000"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HWW21324.1"
                     /translation="MPRAKPMPALLEVQHSMRQRLLVPGPPNADLLQEEVLAIYRNTV
                     TSTLVYALRLSYPAVLRVVGAEFFEGAAHEFIAAHAPRSACLNDYGDRWPDFLAQFAP
                     AATLPYLPDVGRLEWAINRALHAADAAGIDITRLASLGDAALPGVSFAVHPALSLLQL
                     AFPADSIWRAVLDQDSAAMARIDLTSGPVQLLIERDAHGVQVRRLTAWAWEFTRSLAQ
                     GKPLHAALDETAAPEGDALNALLADHLSSGRFIDFSCTGEPKS"
     gene            complement(5688..6569)
                     /locus_tag="VNZ06_11005"
     CDS             complement(5688..6569)
                     /locus_tag="VNZ06_11005"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HWW21325.1"
                     /translation="MNESIRYSSATSPVPVSAGIGLRFPHHRQVLETTPPVAWLEVHS
                     ENFFGGGTVRQTLLNVRRHYPVSLHGVGLSLGSAEGLDPGHLRRLADLVDIVEPSLVS
                     EHLSWSVVGGEYLADLLPLPMTDEALQVVCRHVQQTQETLQRQILVENPSTYLQFQHS
                     SIPEWEFIAEVARRTGCGLLCDVNNIYVSACNHGWDADLYLRNLPARAIGEIHLAGHA
                     VRTLDSGRTIRIDDHGSRVAPEVWSLFEQALRLFGPKPTLIEWDTDVPALGVLLDEAG
                     LANERLRAARQADACAA"
     gene            complement(6608..6895)
                     /locus_tag="VNZ06_11010"
     CDS             complement(6608..6895)
                     /locus_tag="VNZ06_11010"
                     /inference="COORDINATES: protein motif:HMM:NF021559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HWW21326.1"
                     /translation="MNRLVLAASALSAAVGLALAVSPIASADPVKDNTAKAMKDHLEK
                     CYGINASGKNDCATGSHSCAGQSTTARDPKSFVLVPSGDCAKIAGGTTSAM"
     gene            6959..>7330
                     /locus_tag="VNZ06_11015"
     CDS             6959..>7330
                     /locus_tag="VNZ06_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWW21327.1"
                     /translation="MQKVTLDAVATDSTPAERDLAWSRLMRAAQAGERAAYERLLREV
                     TPLIRSVVRRHSNNRADIEEIVQDTLLTLHRVRHTYDPDRPFGPWLAAIASRRSIDAL
                     RRRIRLAKFEAPEQGAYETFAD"
BASE COUNT         1485 a         2296 c         2257 g         1292 t
ORIGIN      
        1 ctggtcaccg ccatccaact cgaccgccag accagcattg ccgcccggca gcgggaacag
       61 atcggtggtc gtgagctgcg cccgtgcgaa gctctcttcg gtctgagagt aggaattgat
      121 ctgaccgaaa aagctgtcgt actgcgccgg ggtcagcgcc gtatagaact tcgcgtagtt
      181 cggcgtgtac tcgaaatcac ccagcgggcc gacaccgagc tgtggtccga agatgttcgc
      241 gaagaagctt tcgacctgcg gagcaaacaa caaactggtc cgctccgtca gcttgttcct
      301 cgtgtaggtg aagtcggcga tgtacttcca gctcgacagg aaatcaccct gcacgccgag
      361 tgtggcccgg atcgaatcat tggtgtcctt gtcgtcgttc ttgtcgcggc cgcccgactc
      421 ctcgggtgag aagatgtgct gcacattgta atagtcgggg gcaccaccat tgttgacgtt
      481 gatggcgtcg agattcggat cctcgtagta gtagaacggg ctggtggcgt cgacgctgct
      541 accgtagaac agctgtccga cattgaactt ggacacgtcg tggttgacca ggctttccac
      601 gaaaatctgc agggtgtccg tgacgtcgta ggtcgcatgc agatagccct gcgtcgattc
      661 gtcgccgttg gcgacggtgt agaagcccga cctggtggta ccgcaatacg aacctcggtt
      721 ctgacgggtg tacaaccgct cgctaccgcc gaacccctcg gtcgtgttcg cgcaattgtt
      781 gggatccggg aagtagtaca aatcaccgtt gggctggcca aacagaccga ggaccagata
      841 gtcgcgctcg gcggtctgcg gtgtggtgcc ctgggtgaag tactggctgg tcagcggacg
      901 ctgatacccc cagatcggcg acaggttctc gtattgaccg ccgatcacca cgttgaagtt
      961 gccgatggta aagccatcgc caatcgcgaa gcggcgatca ttgccaccgc catccgcggt
     1021 ccatccgtag cgggcatcca ccaccggacc gtccagattc ttcttcatga cgacgttcac
     1081 gacgccggcg atggcgtcag acccgtagat cgaggactgc gcgcccggga gtgtgtcgat
     1141 gtgatcgacc agcactgtgg gaatgccaga gatgctgacg aagttctcgg agccgttgta
     1201 gagcgccgga tagtcagcga tcggacgtcc atcgatcagg tatttggtga agctgacgtc
     1261 caggccgaat agcgaggtta ccttcgcggc ctgggtgaaa cctgcgaact ggccgttctg
     1321 gatactgccg gtggaaaatg agctgcgctg cagcgcttcg gcaatatcgg cgaatcctct
     1381 cgcctggatg tcttcgtttg agatgaccgt gaccggggtc tggatcgaga catccgtttg
     1441 tggaatcaac gagccagtca ctaccacttc tttcaattcg gtctcgtgct tggtgcccga
     1501 ggcgtcgcca gtcgttggct ggtcgttgtt attattgttg tcggctgccg ctgaaagagt
     1561 ccaaggcaat agacctaaag caagcatcgc aatggcggca ctcgatgctt tgcgaaaaga
     1621 aaaatccaac cgaggtacgc gggtacccat agtgttttct ccctagttct agttctcgat
     1681 gaagccgctt tcgcctcgtc gtgcgggggg cgatagtcgg aaatccatcg tgttgcaatc
     1741 accgtgcaac tcgacagtag cacatagttt tgcccgagtg catccgttgt acgggtacga
     1801 caacggcccc gcagagggga cctgaatttc cgcgcggaaa ggacgcgcgg cgcacggtgg
     1861 gcaacgcttt acggttgctt ttcagatcgc gcgagtcgcg gctgctgcgg gctcgccgcg
     1921 caattgctgc aacccggcgc gctcaaccgc gcgcgatgcc gacgtgaaac ctcgatcagc
     1981 agtccacgct tggccagcgc gtcgagcacg cgctgacgtg ttggcatcga caggaatgac
     2041 caggtgacgt agccggcagc ggccagcacc acgagtccaa tgagtagctg ctgccacatg
     2101 gatcgaactc aagctcccaa cgccagcgcg acgcggtagg ttaccaacgc tgcgatatac
     2161 gccagcagga acagatacgc cgccatgatc agcggatagc gccacgagtt ggtctcgcga
     2221 cggaccgtca cgagtgtgga cagacactgg ggtgcgaaga cgtaccaggc cagcaatgac
     2281 agcgcagtgg ccagactcca gctgccggcg atcatcggct tgagcgcgcg ctcgacgtct
     2341 gcgccgccat tgccggacag cgaatacacc gttcccagtg cgccgaccac gacctcgcgc
     2401 gccgcgatgc cgggcaccag tgccacgctg atctgccagt tgaaccccag cggcgcaaac
     2461 agatgttgga gcccggcacc gagcatgccg gcgaagctat agcggattgc gggcccatcg
     2521 gcgccgctcg gtggcgccgg atagctcgcg agaaaccaca gcagcaccat cagcgccaga
     2581 atgatggtgc cgacccgtgt cagaaagatc tgcgttcgct cccacagacc ggtcaacaga
     2641 ttgcgcacag tcggccagtg atactcgggc agttccatca ggagtgggtg atagttggag
     2701 cgcagcgtgg cgcgcttcat gaccagggca accagcagcg cgccagcgac gccgagcaca
     2761 tacagcgaga acagcaccag tccctgcagg ttgaaaatgc cgatgctgcg agccggaatg
     2821 aacgcgccga tgatcagcgc gtacaccggc aggcgcgctg agcaggtcat catgggcgcc
     2881 agcagaatcg ttgccaggcg gtcgcgcaga ttggggatgg tccgtgtcgc catgatccca
     2941 gggatcgcgc aggagaaaga agacagcagc ggtatgaaag aacgccctga aagtccgacc
     3001 cctcccatca gtcgatccag caggaaggcc gcccgcggca ggtagccgct gtcctccagc
     3061 agcaggatga agaagaacag gatcaggatc tgaggcagga ataccagcac actgcccgca
     3121 cccgcaatga cgccaccgac gatcaggctg cgcagcggac cctccggcag cagcgcctgc
     3181 accgcatcgc ccagcgccgt gatgccggca ttgatccagt tcacaggcga tttggcccag
     3241 ctgaataccg cctggaacac caggaacagc aatgcagcca gcaggatcgg gcccagcacc
     3301 ggattcatca ccaccgcatc gatgcgtgcc agggcgcgat cgcgcaacgg ctcgacgtag
     3361 cccaccgctg agaggatgcg gcgcgcctct cgctgggtgg cagccagttc ttcgtgcgag
     3421 cttccgggga gcgtgaacgc ctggcgcgcg ggacccacga ccacctgatc gagtgccgca
     3481 atcaggttcg cctcgccgcc tgacttgacc gcgaccgttt ccaccaccgg cacacccagc
     3541 tcgcgcgaca gagctggcgc atcgagcatg aagccacggc gatgcgccag gtcgctcatg
     3601 ttgagagcga ccaccattgg aatggacaag cgcgccagct cgagcgccaa ccgcagcgac
     3661 agcctgaggt tggtggcatc cagcacgcat acgatcagat ccggcggcgt ctcgccgttg
     3721 agccgcccga gcaccgcatc gcgtgtgatc gcctcatcca gtgaggtcgg caccaggctg
     3781 taagtccccg gcaggtccat gacgcgatag agccattgcg gctgcgcgcc gacgaaagcg
     3841 ccttccttgc gctcgaccgt gactccagga tagtttgcga ctttctgccg gctgcccgtg
     3901 agccggttga agagcgccgt ttttccgcag ttgggcgctc caaccagcgc caccctgcgc
     3961 acttgaatat cgggattgtc tcgcgtcacg tcgcctcgcg acttagttaa ctggtagcgt
     4021 caggcctcga cctgaaccag gaccgcctgc gcctcgcgac ggcgcaacgc aaacatcgag
     4081 cagccgatgc gcaccgcgat cggatcgccg ccgggccgga cttcctcgat gacttcaatg
     4141 cgctcaccgg cgataaaacc cagttccatc agtcgccggg ccacggtgga gcccaccgcg
     4201 tccccgagtg agtggccggc atcgaggtcc gatatcgcac tgacaatgcc acggcagccc
     4261 ttgcgcaatt gcgccaaggg ttgaatggcg tcggtctggt gccatgaacg gcaggaatcc
     4321 acaggagcgc tcgcctcacc gcgtgaatga caggaaagta acaaatgcga atcactcgca
     4381 atcgcgtttg cagcatatcc cacgcggctc tcctgcgcca cttcagtagc gtcaggatga
     4441 gcgatcggtg gagcgctatg tcaaatcgct tccccgatgc ccagccagcg cttgatcagg
     4501 tagtcgagcg agaccttgcc gggacctcgg cagagcagaa acaccagcat cgcagcccat
     4561 tggatgtgtg tcggccacgc tcgtgggtag ataaaggctt cgatatacgc gctcatgccc
     4621 agcagcagga acgcgactgg acgggtcagc aggcccaaga ccagcagtat cgagctcgtc
     4681 gcttcaatgc cggcggtcag atgcgcagcc gcatccggag agagcagcgg cagtttgtac
     4741 tcgtcctcga acagcgccac cgtcgtgtcc cagttctgga ttttggtcga aaacgaactc
     4801 cagaagacct cagccaccgc caggcgtgcg ggtatcgcca gcaggctgta cgggacgcgg
     4861 tccagccaat tcaggatgcg ctcgatcagt ccggtcaacc ctgactggga tgctgcagcg
     4921 ccatggctgg tggtattcac gacttcggtt ctcctgtgca cgaaaaatcg atgaatcgtc
     4981 ctgaactcag atgatcggcc agcagtgcat tgagcgcatc gccttcgggc gcggcggttt
     5041 catcgagtgc ggcatgcagc ggtttgcctt gggccagcga tcgggtgaac tcccacgccc
     5101 atgcggtgag acgccgtacc tgtacgccat gcgcatcgcg ctcgatcaac agctgcaccg
     5161 gtcctgacgt cagatcgatc cgcgccatcg ccgcgctatc ctggtcgagt accgctcgcc
     5221 agatactgtc ggcgggaaag gccagctgca gcaggctcaa cgccgggtga accgcgaaac
     5281 tgacacccgg cagcgccgcg tcacccagtg acgccagacg cgtgatatcg attccagccg
     5341 catcggcagc atgcagcgcc cgattgatcg cccattccag gcgcccaacg tccggcagat
     5401 agggcagcgt cgcggccggt gcaaactgcg cgaggaaatc gggccaccga tccccatagt
     5461 cgttcagaca ggcgctgcgg ggcgcatgcg ctgcaatgaa ttcgtgcgcg gcaccctcga
     5521 agaactcggc gcccaccaca cgcagcacgg cggggtagga cagtcgcagt gcatagacca
     5581 gcgtgctggt caccgtgttg cgatagatcg ccagcacttc ctcttgtagg agatccgcat
     5641 tcggcggccc cggcaccagc aggcgctgac gcatcgagtg ctgtacttca agcagcgcag
     5701 gcatcggctt ggcgcgcggc acgcagtcgt tcgttggcca gtccggcttc atccagcagc
     5761 acccccaagg ccggcacatc ggtatcccac tcgatcaggg tcggcttcgg cccgaacagg
     5821 cgcagcgcct gttcaaacag cgaccacacc tcgggcgcga cgcgcgaacc atgatcatca
     5881 atgcggatcg tgcggccact atcgagcgtg cgcacggcat gtcccgccag atgaatctca
     5941 ccgatcgccc tggcgggcag attccgcaga tagagatccg catcccagcc gtgattgcag
     6001 gcgctgacgt agatgttgtt gacgtcgcac agcaggccgc atccggtgcg gcgtgcgacc
     6061 tcggcgatga attcccactc cgggatgctc gaatgttgaa actgcaggta cgtggacgga
     6121 ttctcgacca ggatctgccg ctgaagggtc tcctgggtct gctgcacatg tcggcagacc
     6181 acctgcagcg cctcgtcagt catcggcagg ggcagcagat cagccaggta ttcgccaccg
     6241 accacgctcc aggaaagatg ttcggagacc agcgagggct cgacgatatc caccaggtcg
     6301 gccaagcgcc gcaggtgccc gggatcgagg ccctccgcgc tgccaaggga aagtcctact
     6361 ccgtgcagcg ataccggata gtggcgacgc acattcagaa gcgtctgccg tacagtgccg
     6421 ccaccgaaaa aattctcgct gtgcacttca agccacgcga ccggaggcgt ggtctcgagc
     6481 acctggcgat gatgtggaaa gcgcagcccg atacccgcac tgacgggtac cgggctggtt
     6541 gcgctcgagt agcggatcga ctcgttcaat ggacgcactc aagcttgagg cgacaccctg
     6601 cgaatgctta catcgcgctg gtggtgccac ccgcgatctt ggcgcagtcg ccagacggga
     6661 ccagaacgaa cgacttcgga tcgcgcgccg tggtggactg accggcgcag gaatgagagc
     6721 cggtagcgca atcgttcttg ccggaagcgt tgatgccgta acatttttcg agatgatcct
     6781 tcatcgcctt cgccgtgttg tcctttaccg gatcggctga agcaatcggc gacacggcga
     6841 gtgcgagacc cacggcagcg gacagggccg aagcggccag taccaaacga ttcatgatga
     6901 tcccctcttt gactgacggg ccccgggatt ggggccacgt ggttaacttc gtggatacgt
     6961 gcaaaaggtt acactggatg cggtggccac cgattctacg cctgccgaac gagacctggc
     7021 ctggtcccga ctgatgcgcg cggcgcaggc cggcgagcgc gcggcttacg agcgactgct
     7081 gcgcgaggtc accccgctca tccggtcggt cgtgcggcgc cattccaaca accgggcgga
     7141 catcgaggag atcgtccagg acaccctgct gaccctgcat cgtgtccggc atacctacga
     7201 tccggatcga cccttcggtc cttggctggc ggcgattgct tcgcgccgaa gcatcgatgc
     7261 cctgcgccgg cgcatacggc tagcgaaatt cgaggccccc gaacaagggg cctatgaaac
     7321 ctttgccgac
//
LOCUS       DATMRM010000365        17739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2380896, whole genome shotgun sequence.
ACCESSION   DATMRM010000365 DATMRM010000000
VERSION     DATMRM010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17739
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2380896"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VNZ06_11020"
     CDS             <1..529
                     /locus_tag="VNZ06_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWW21328.1"
                     /translation="EAWLGAESGSQRILDAMNKGTTIQEILTARDRLRSCGIRVGFFI
                     QLGYLGEQLEDILATRSLIEQARPDAVGVSVSYPLPGTVFYNQVRQQLGRKTHWRESN
                     DLEMMFHGTYVSDFYRAVRDLLHEHVSAERLSALPNSEPHRHALQALRTRWEDLVARE
                     QQYRRDRSLVSVALN"
     gene            532..1797
                     /locus_tag="VNZ06_11025"
     CDS             532..1797
                     /locus_tag="VNZ06_11025"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWW21329.1"
                     /translation="MPPLRLVESALRQATEAFAAELASPGNRSPEWGELEWTMAKAAA
                     VLHGVAPLLAASLHWQGPPEWRRFVEQQNHHTLLRHRRIEALLACMDEATRRQGIAAL
                     ALKGAALHALGLYCAGQRPMADIDLLVRERDVQPMLRVLQSLGYQQTSATWKHRILEP
                     QRPAAVSRPQAQYALGEHSEAAIKIELHTRIAERLPVIEVDATECIVPSEPRPGLVGY
                     PSTAALLMHLLLHAAGNMVVRTLRLIHLHDIALLTARMNDAQWAQWLRSRVDHRSAWW
                     AVPPLELAARYYPHSIPSEVLAELRPECPRALRALSRRQTLSDVSYAALDVEAFPGLG
                     WAASLSEKWRCILARIRPGADWRVMRELVRAEPWSATGASPRQSLGRRGLRILLTRPP
                     RPASMYIVRAALASMTIHEIRSTPSHGPL"
     gene            1810..2607
                     /locus_tag="VNZ06_11030"
     CDS             1810..2607
                     /locus_tag="VNZ06_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWW21330.1"
                     /translation="MKVSIIIAVYNEAPTVAALLDRVWLQPLPGVTKEIIVVESNSTD
                     GSREIVADFLGSHGDESSARIQVIYQDRPKGKGNAIRQGFSVASGDILLIQDADLEYE
                     VSDYPDLLAPIISGRAAFVLGSRHLGAECWKIRRFAHHGLKAAFMNLGGMLFHGFFNL
                     LFATRLTDPTSMYKVFRVDCLEGLRFTCNRFDFDFELLGKLIRAGFIPLEIPVSYASR
                     GFEQGKKIRIFRDPWTWVAAILKCRFSAIKGTAHATVPQGDAVRDNA"
     gene            complement(2634..4112)
                     /locus_tag="VNZ06_11035"
     CDS             complement(2634..4112)
                     /locus_tag="VNZ06_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21331.1"
                     /translation="MSRKADRPFGLGRYDAAVLLGSVIYLYANLLTSAHTPLLLGGDE
                     QVFWMNAQRLLHGELVYRDFFEFTPPGTDLAYLAAFRLFGSQVWVTNLVVLLLGAALS
                     WLCYRVACSVLRPALAAVTAALFLVFDYGSWLDGTHHWFSMLAVMGALAALLSRTSNS
                     KLLICGALLGTASFFTQTRGAMAAMGVAGYLVWERFQTRDSWFHCLRRQSLLALSFLS
                     AWGVLSSYFIFRVGFPRLWYFQFTFVRQYMRNQWNGLPPDSVLSWPLRYSIAYFAMPA
                     VCSLALWKLLRTQRDMSSLCQRRIALLAFVGAALFLEVAQSPNWLRVYCVAIPSFVLA
                     MWLICDQVGSYRPHLTGLLCVGLVVYAAHQAWVRQLEHSVTVDLPGGRVAAVPLAGEK
                     LGWIAKHTEPGQYFFQSRLPGVYLPLALRIPTFTFLFHETRPEFLELSMRQLEQKRVR
                     YILWSPGLETPWYLFATFHQFLLDHYHKVWTFSDQEEIWERN"
     gene            complement(4109..5410)
                     /locus_tag="VNZ06_11040"
     CDS             complement(4109..5410)
                     /locus_tag="VNZ06_11040"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HWW21332.1"
                     /translation="MGIRGKMKHPAVFLDRDGVLNRALIREGTPHPPASVAELEILPN
                     VPEALDALRARGYALIVVTNQPDVARGTASKQMVIDINNHLKDRLNLDAVLTCFHDNA
                     DNCDCRKPRPGLLLRAAQDLGIDLSSSFMVGDRARDVEAGQRAGCRTFFVDGNYMEAP
                     PSRCDFRVGSLIEAANIILSTSARQKVTLDQLNVKLFADGANKAGMLDMCAKPYIKGL
                     TTNPTLMRKAGISDYRAFAREMLTEIRDKPISFEVLSDDFAQMEREALEIASWAQNVY
                     VKIPVTNTRCESCRSLVSRLSNRKVKVNVTAVMTLAQVRGIVEALNPAVPSYVSIFAG
                     RVADTGLDPLPLIAKALELLKLNSKAELVWASSREILNIFQADAIGCHVITVTQDILN
                     KLSLIGYDLTQYSLDTVKMFCDDAQSAGFSLEGSPLSTTAP"
     gene            complement(5389..5988)
                     /locus_tag="VNZ06_11045"
     CDS             complement(5389..5988)
                     /locus_tag="VNZ06_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWW21333.1"
                     /translation="MSYTQEYLAEAVAILQRLDVAAIERLVDSLARIKASAGRIFFLG
                     VGGSAANCSHAVNDFRKLVGIECYAPTDNVAELTARINDDGWESTLVEWLKISRLGAK
                     DAVFIFSVGGGDAEKNISRNLVAAADHAKAVGASVFGVVGRDGGHTARVADACVIVPT
                     VNPQTITPHTEAFQALIWHLLVSHPRLKPKQTKWESVGK"
     gene            complement(5985..6692)
                     /locus_tag="VNZ06_11050"
     CDS             complement(5985..6692)
                     /locus_tag="VNZ06_11050"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWW21334.1"
                     /translation="MLPLAILAGGLATRLGPVTSRTPKSLIAVAGRPFIYHQLELLRS
                     QGIDRVVLCVGHLADQIRASVGDGNGLGLTIRYSYDGDRLLGTGGAIKKALPLLGTDF
                     FVLNGDSYLPCSLAQIQSAYRSAGRPGLMTLVRNDNRWDRSNIVYRGGEIVEYDKHSI
                     RADMAHIDFGLSVFSKDVFAKYQPARSIDLADICRELVTSGQLAAFEVSGRFYEIGSQ
                     QGIIETQEFLSRRPANA"
     gene            complement(6695..7642)
                     /locus_tag="VNZ06_11055"
     CDS             complement(6695..7642)
                     /locus_tag="VNZ06_11055"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21335.1"
                     /translation="MGGGGTDLASYYAEREGFLIAAAIDKYVYVTVMRPFSPGIYLKY
                     SALEHVERIADVKHPIIREALAMQDMPNPQIEITTLADIPAGTGLGSSGSFTTALLKA
                     LYAYQRRVAHSQELAELACHIEIDRLGEPIGKQDQFIAAFGGLTCLTFHKDHRVTVQP
                     LRIGAKTMYDLEDNLLLFFTGSNRAAGSILKDQHERSLKREDDMIRNLDYVKALGHRS
                     REALESGDLGRFGELMHEQWEHKKRRCGSASNLKIDQWYSLAMKNGAVGGKLVGAGGG
                     GFLMFYAADRARLRYAMSQAGLEEVRFRFDFEGTKVVLS"
     gene            complement(7659..8648)
                     /locus_tag="VNZ06_11060"
     CDS             complement(7659..8648)
                     /locus_tag="VNZ06_11060"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWW21336.1"
                     /translation="MNVGIIGCGAVGQRRSQHLAGANLIHCADTDLPQAQRVAQRAPA
                     CACSADWRELLARPDIDAVVVATPPDLQAPIVRAAISAAKHVLVEKPAGRNVDELQEL
                     RRLAAEHDVRVRVGFNHRYHRAFRQARRLVDAGALGPLLFVRARYGHGGRVGYEQEWR
                     AQPQRSGGGELIDQGVHLIDLARWFLGEFVEVDGFAHTYFWNMPVDDNGFMLLKTATK
                     QVAFLHASCTEWKNIFSWELCGRDGKLQVDGLGGSYGMERLSWYKMLPQMGPPETTIW
                     EYPMADDSWSIEMEEFIEDVRSGREPAAGLQDAMAALAVVARIYADSGYDYRA"
     gene            complement(8645..9523)
                     /locus_tag="VNZ06_11065"
     CDS             complement(8645..9523)
                     /locus_tag="VNZ06_11065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWW21337.1"
                     /translation="MTRQLENRCAIITGASQGLGLEIARHYLAAGANLMICARGQEPL
                     QAACSTLRALAGVNQAVVAMTADVSDPDAVTTLVERTMSEFGRLDVLVSNAGIAGPSG
                     AVESVDWREWTRAIEINLLGPVLLSRAVLPHFKKAGRGKILQLSGGGATTALPMLSAY
                     AASKAAVVRFMETLAEETRSYHIDVNAIAPGVLDTRFLDGFIAAGPDKIGREFYERSL
                     QHKRNGGVPLSKGAELAVFLGSSASDGITGKLISAVWDPWRILPDHRDELNRTDIYTL
                     RRIQPRDRQMGWGEVD"
     gene            complement(9529..9708)
                     /locus_tag="VNZ06_11070"
     CDS             complement(9529..9708)
                     /locus_tag="VNZ06_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21338.1"
                     /translation="MTIRVLGRAVADFLHPDRVVVGIRSAQDREVLAGAYVSPGAGLP
                     AHLRYLDVSMPVKEP"
     gene            complement(9705..10676)
                     /locus_tag="VNZ06_11075"
     CDS             complement(9705..10676)
                     /locus_tag="VNZ06_11075"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWW21339.1"
                     /translation="MQEGQLRYLVTGCAGFIGSTLTQRLLATGHSVVGYDNFSTGQED
                     FVRSARQHANFHLVRADVLDLKTLSDSMAGSELIFHLAANADVRFGVEHPRRDLEQNT
                     IATHNVLEAMRRNGIDRIAFSSSGSVYGESSIAPTPEDAPFPVQTSLYGASKAAAEGL
                     IAAYSAAFGFQAYIFRLVSILGERYTHGHVFDFYRQLMADPGRLYVLGNGRQRKSYLY
                     VGDCIDAMLLAIERANDRVNVFNLGHDGFCEVNDSIRWICAALGLRPEIVYSGGDRGW
                     VGDSPFIFLDTHRIRALGWKPKRSIQQGVLATLEYLRSNPWLLAARQ"
     gene            10781..11965
                     /locus_tag="VNZ06_11080"
     CDS             10781..11965
                     /locus_tag="VNZ06_11080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HWW21340.1"
                     /translation="MHAVAPALTRLDTDFPDYLTAARIVADRGEVDRLYDNAWFQDQM
                     RRYHIGKPSEGKFAPFPPPTALLWVSLARLTPLDALRVVTGLSVLALAVSVTLLSRIL
                     GWSVLDSALLVLLSGVAAINAVRLGQPYMLVSLSCILGYYAYLRGRPLLAGLCFGLFT
                     PIKYFPVVILIYFAFCKQWRVVLGGAAAILAVVLVSIGLLGWKVHQEFLSSVLGMHLV
                     GQLSMQDPFTASFQSFDTLFRRLFVFDATSNPHPMLSSPELSGPAVAITKSLIFCTAI
                     ATLIRLARSAGERAPPASIGLLGILVLLLAPATATYHFVLLWLPIGLLINFLLREGAR
                     AFAYFILGVYALIGFFPYRLSVHFEGDGALTLLAYPRLFLLLAMFMACVIFVWRPRRG
                     MA"
     gene            11962..13500
                     /locus_tag="VNZ06_11085"
     CDS             11962..13500
                     /locus_tag="VNZ06_11085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWW21341.1"
                     /translation="MKTRQQNLVSYAALLLLGSLAFIHLMALPPFQDEGSQLRWVWRL
                     INAGDWRPSLSEGKPLEAWLVAPLALAGFAPLAAARTLHVLAGMAGTGLIYQLALQFG
                     DRTRAFLSALLFTICPFVVYLQRLALSDIFLCTAGIWVLLSIARLLKSPTWSHSLVTA
                     IALLLAALCKLPVGFVFLTSMPLALLFMPSSERQVLLRRPELTKLLAAFVPVLLLLLI
                     VAFTAMIQLRRGHAPGFGVADLMGIGMGRYVELARVLGVQRSSLLAELTTQMSWPVVI
                     IGLIGIVGGALSGDWHDRWLIAAGLLPMLAVAWLADFWFPRYLLFTLPPLIVAAVAGW
                     QFLSTRARSLARTVELVALTACMVFMGHQSALIILNPIAASWSPVDRTQYIEGGGSGY
                     GYPQAAKYLLQANDVPQVIYSLDGHSAFQLLTYLPAAWNSRVKTISYAQDGQELRSQE
                     ARFENLLEHAPAWIVVPDPVLTSYLESSFGMMRLNRITLRPVVRFDKPGGHEQLAIYE
                     VTRR"
     gene            complement(13521..15113)
                     /locus_tag="VNZ06_11090"
     CDS             complement(13521..15113)
                     /locus_tag="VNZ06_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21342.1"
                     /translation="MKLSRLSRVSRSPLTAPVKWWTAFVCLVIVARAVWILSGGPRRI
                     VFFVPDDAFYYFITATNFADTGEWTFDHGLSHTTGVQWLHAYALAGLSWLMKPKPGLR
                     LVYLAIVLSTACAVAGAVVTVRFVRRTCGEVAAASLALPLLSMGFLKGASSGMEWGWV
                     VLISSIIFAIFSRLGSAPLPRRQALGLLALGLLLELARSDAGALAAATLAGTGLVACW
                     RPNRPAMHGAGMLLAGATLGLLLTLGHTYALSGHLLSGSVRIKMLWGTRSSFSLRPFG
                     DLLLSAIGIPQRGRSTPVLAVLAVLILAAAAAPALRKPIPWLQALTLQQANLCALSLC
                     SLLIYSLLFGLNPSVLSWYTSLIALPVCVLVAACSQGAWRAGRPAMQMVAAVFWCALV
                     GYAVIDGFIERGHMQLLYYKAALRLGQELPGATVGCWSCGILSFFSAGPGRPPLINLD
                     GLMNDDVYPYIEANDLASYVVGNRIHYVLDESAYIEERRARVLGGYDSEQFLKMLTVI
                     ERFPYSETATDSTVPSLYRLDY"
     gene            complement(15124..16293)
                     /locus_tag="VNZ06_11095"
     CDS             complement(15124..16293)
                     /locus_tag="VNZ06_11095"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWW21343.1"
                     /translation="MDATAAHRAASSIGKVRELDGLRGLLSLLVVLNHLNGSSLPWVW
                     GCMDLFFALSGYLIGRGSLQHRHQPNFWWVYLRRRVVRIWPLYFAVLGFACAAAWIDV
                     HYFHAPEESLSWRVLQCVFFVQNSEFYWQPFTNGWGPGYPIMFGHAWSLALEEQFYLL
                     APLLAWLMFAAGTWLTKRSGWLPWAVLALFLIACNLLRAEGIYTFLLLGRLDAFVLGT
                     LVAVVEARSHAEPASSARAGRWFSALLIPGGGMAAVLALYSYSPSAPGGPLGLNAIPG
                     VSVFAIFAAVLIGAIIFRRAPGMTAFLGWGPLRYAGTISYSTYLWHVPVMVLMTRMFW
                     RASWIPGGYGLHAGLMASVSLTIAHFSYRLIEAPMLRAKPDYDRAYAGQPAQSPA"
     gene            complement(16313..17704)
                     /locus_tag="VNZ06_11100"
     CDS             complement(16313..17704)
                     /locus_tag="VNZ06_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWW21344.1"
                     /translation="MSTMRVTGYSTLWRLAALLCAMALSSCATAPRNAVGGNHDDGAL
                     NASADRLIVVTVDNRTRPLIGEPGSTPHGYSATNPYTVSDQARAVTAALAHDYGLREV
                     REWPILPLKVQCLVFAVPPSADRVALLRRLSADGRVKLAQPLQLFSALADGDVGNSSV
                     ARPELRVAPISGSPYNDPYYGLQHGFNEIDAAAAQQWSRGDGVSVAIIDTGVATTHPD
                     LDGRIAVIRNFVDEDMKAFLTDRHGTEVAGIIGADANNGLGIVGIAPGVRLLIYKACQ
                     PAHPQSMEAVCNSFTLARALGAAIDAHPQIVNLSLGGPADPLLSQLVSYGVQHGVIFV
                     GAVPESGRLDGFPLDVGGVIAVDQIGRSEHAPGVLHAPGRDIVSTAPGGSYDFVTGSS
                     FAAAHVTGAIALLRARVPAMSAKALSALLERTGVPRQMGDSIDICAALDAVQPSDHCM
                     HSARAVATATAAH"
BASE COUNT         3530 a         5556 c         5288 g         3365 t
ORIGIN      
        1 cgaggcctgg ctcggggccg aaagtggcag tcagcgcatc ctcgatgcga tgaacaaggg
       61 cacgacgatc caggagattc tcaccgcccg cgatcgcctc aggagctgtg gcatacgcgt
      121 cggcttcttc attcaactcg gctatctggg agagcagctc gaagacatac tcgcgacgcg
      181 ctccttgatc gagcaggcca gaccggatgc tgttggcgtc agtgtctcct acccgttgcc
      241 cggcacggta ttttacaacc aggtcagaca gcagctcgga cgcaagaccc attggcgcga
      301 gagcaacgat ctggagatga tgtttcacgg cacttacgtt tcagacttct accgggcggt
      361 gcgtgacctg ctgcacgagc atgtgtcggc cgaaagactg agcgcgttgc cgaacagcga
      421 accgcatcgt cacgccctgc aggcgctgcg aacccgttgg gaagatctgg ttgcgcgcga
      481 gcagcaatat cgccgcgatc gcagcctcgt ttcagtcgcc ttgaactgac catgccgcca
      541 ctgaggctcg tcgagtcggc actgcgtcag gcgaccgagg cgtttgctgc cgaacttgcg
      601 agtcccggga accgctcgcc tgaatggggc gagctcgaat ggaccatggc caaggcggcc
      661 gccgttctgc atggcgtcgc gccactgctg gccgccagct tgcactggca ggggccaccc
      721 gagtggaggc gatttgttga gcagcagaac catcacacgt tgctgcgaca ccggcgcatc
      781 gaggccctgc tcgcgtgcat ggatgaagcc acccgacggc aaggtattgc ggccctagcg
      841 ctcaagggcg cggcactgca tgcgctcggc ctgtactgcg ccggccagcg cccgatggcc
      901 gatatcgact tgctggtacg tgagcgcgac gtccaaccga tgctgcgggt gttgcaaagc
      961 ctgggatacc agcagacttc ggccacctgg aaacatcgaa tcctcgagcc ccaacggccg
     1021 gcagccgtat cgcgtcctca ggcccagtac gccttaggtg aacactccga ggcagcgatc
     1081 aagatcgagt tgcatacgcg gatcgcggaa cggctacccg tcatcgaggt ggacgcgacc
     1141 gagtgcatcg tgccttccga accacggcct gggctcgttg gctacccgtc gaccgcggca
     1201 ctgctgatgc acttgctgct ccatgcggcg ggcaatatgg tggtacggac gctgcgtctc
     1261 attcacctgc atgacattgc attgctgacg gcgcgcatga atgacgcgca gtgggcgcag
     1321 tggctgcgta gccgcgtcga ccatcgatcg gcgtggtggg cggttccgcc gctcgaactt
     1381 gctgcgcgct actacccgca cagcatcccg agcgaagtgc tcgccgaatt gagaccagaa
     1441 tgcccgcgcg ccttgcgagc cctatcgcga cggcagacac tttcggatgt ttcctatgct
     1501 gctctggacg tcgaggcgtt cccgggtctt ggctgggccg cctccttgtc cgagaagtgg
     1561 cgttgcatac tggctcggat tcggccgggg gcggattggc gggtgatgcg cgaactcgtg
     1621 cgagccgagc cgtggagcgc caccggcgcc tcaccacgtc agtcgctcgg tcgccgcggg
     1681 ctgcgaattc tgctgacccg accgccacgg ccagcctcca tgtacatcgt gcgcgcagcg
     1741 ctcgctagca tgacaatcca tgagattcgc tcaacgccaa gccatggccc gttgtaattt
     1801 ccggcatttg tgaaagtatc gatcatcatc gcggtgtaca acgaagcgcc aaccgtggcc
     1861 gcgctgctgg atcgtgtctg gttgcagccg ctgccgggtg tcaccaaaga gatcatcgtg
     1921 gttgaaagca attcgaccga tggttctcgc gagatcgtcg cggatttttt aggcagtcac
     1981 ggggatgagt cgtcagcgcg tattcaggtc atttatcagg accgtccgaa aggcaaggga
     2041 aacgcgatcc ggcaaggttt cagcgtcgcg agcggggata tcctgctgat tcaggatgcg
     2101 gacctggaat atgaggtctc ggactatccc gacttgctcg caccgatcat ctccggccgc
     2161 gcggctttcg tgctcggcag tcgtcatctc ggagcggaat gctggaagat tcgaagattt
     2221 gcccaccacg gactgaaagc ggcgttcatg aatctcggcg gcatgctgtt tcatggtttc
     2281 ttcaatctgc tcttcgccac ccgcctcacc gacccgacat ccatgtacaa ggtgtttcgc
     2341 gtggactgtc tcgagggcct gcggttcacg tgcaaccggt ttgatttcga tttcgagctg
     2401 ctgggcaagt tgattcgtgc gggcttcata ccgcttgaga ttcccgtgtc ctatgcatcc
     2461 cgtggattcg agcaggggaa gaagattcgc attttccgag atccctggac ctgggtcgcc
     2521 gccatactca aatgcaggtt cagcgcaatc aagggcactg cacacgccac cgtgccccaa
     2581 ggcgatgcag tgagggacaa cgcctgaacg ggccttggga gaaggcttgc tagttagttt
     2641 cgctcccaga tctcttcctg atcagagaac gtccacactt tgtgatagtg atccagcagg
     2701 aattgatgga aggtcgcgaa caggtaccac ggtgtttcca gaccgggtga ccagaggatg
     2761 tagcgtactc gcttctgttc caattgccgc atgctcagtt cgaggaactc gggacgcgtt
     2821 tcatgaaaca gaaaggtgaa cgtcgggatg cgaagtgcca gcggcagata cacgcccggc
     2881 aggcgcgact gaaaaaagta ttggccaggt tccgtgtgct tcgcgatcca acccagcttc
     2941 tcgccggcca gcggaaccgc ggcgaccctt ccgccgggca ggtccacagt cactgaatgc
     3001 tccagctgtc gcacccacgc ctgatgtgcg gcgtaaacca ccaatccgac gcatagcaat
     3061 cccgtcaaat gcgggcgata gcttcccacc tgatcgcaaa tcagccacat cgccagcaca
     3121 aagctgggaa tcgcgacgca atagacccgg agccaatttg ggctctgcgc gacttccagg
     3181 aatagcgctg cgccgacgaa tgccagcagc gcgatccgtc tctgacagag cgaggacatg
     3241 tctcgctgcg tacgcaatag cttccagagc gcgagcgagc acaccgctgg catggcgaaa
     3301 taggcgattg aatagcgcag cggccagctc agcacactat ccggtggcag cccattccat
     3361 tggtttctca tgtattggcg tacgaacgtg aactgaaaat accaaagcct gggaaacccg
     3421 accctgaaga tgaagtagct gctgagaact ccccaggccg atagaaaaga cagcgcgagt
     3481 agtgactgac gcctcaagca gtgaaaccat gagtcgcgcg tttggaatcg ctcccagacc
     3541 agatagccgg cgacgcccat tgccgccatc gcgccccggg tctgcgtgaa gaaagaagct
     3601 gtgcccagca aagcgccgca gatcagtagt tttgagttcg atgtccgcga cagcagcgcc
     3661 gccaatgcgc ccattaccgc cagcatgctg aaccaatgat gggtgccgtc aagccagctc
     3721 ccgtagtcga acaccaggaa cagcgcggcc gtcacagcag ccagcgccgg tctcaagact
     3781 gagcaggcaa cgcggtaaca gagccaggaa agcgctgcgc cgagcaacag cacgaccagg
     3841 ttggttaccc agacctgcga gccgaacaat ctgaacgccg cgagataggc caggtcggtg
     3901 cccgggggag tgaattcgaa gaagtcgcga tagacgagct cgccatgcag caaccgctgt
     3961 gcgttcatcc agaagacttg ttcgtctccg cccagcagca agggtgtatg cgcagaggtc
     4021 aacaggttcg catagaggta gataacgctc cccaggagca cggccgcatc atagcggcca
     4081 aggccgaatg gcctgtccgc cttccggctc acggcgctgt cgttgaaagc ggcgagcctt
     4141 ccagcgaaaa tccggcactc tgagcgtcgt cgcagaacat tttcacagta tcgagggaat
     4201 attgcgtcag atcgtagccg ataagcgaca gcttattgag aatgtcctgc gtgaccgtaa
     4261 tcacatgaca gccgatggcg tcggcctgaa agatattgag tatctcgcgc gaactcgccc
     4321 acaccagttc cgcctttgag ttgagcttca gcagttcgag ggccttggct atcagaggta
     4381 agggatccag gccggtgtcg gcaacccgtc cagcgaagat cgagacgtag cttggaacag
     4441 cagggttcag agcttccacg attcctcgca cctgtgccag agtcatcacc gcggtgacat
     4501 tgaccttgac cttccggttc gagagtcggc tgactagtga acggcaggat tcgcaccgag
     4561 tattcgtaac gggaattttc acgtagacgt tttgcgccca gctcgcgatc tcgagggctt
     4621 cgcgctccat ctgcgcgaaa tcgtcggaca gtacctcgaa cgagatcggc ttgtcgcgaa
     4681 tctcggtgag catctctctg gcaaaggcgc gatagtcgga aatcccggcc ttgcgcatca
     4741 aggtcggatt ggttgtcagg cctttgatat acggtttggc gcacatgtcg agcatgcctg
     4801 ccttgttcgc gccgtcggcg aacagcttca cgttcagctg gtccagcgtc actttctggc
     4861 gagccgatgt cgacagaatg atgttggcag cctcaatcag agagccgaca cgaaaatcgc
     4921 agcggctggg cggcgcttcc atgtagttgc catcgacaaa gaacgtccgg cagccggcgc
     4981 gctgtccagc ctcgacgtcc cgggcgcgat cacccaccat gaagctcgaa gacaaatcga
     5041 ttccgaggtc ctgcgctgcc cgaagcagaa gtcccggcct gggctttcta caatcacaat
     5101 tgtcggcgtt gtcgtgaaag cacgtcagca cggcatcgag gttgagtctg tccttcagat
     5161 ggttgttgat gtcgatgacc atctgcttgg aggcggttcc ccgggcgacg tcaggctgat
     5221 tggttacgac gatgagagcg tagccacgtg cacgcagggc atccaaggct tccggaacat
     5281 tgggcaggat ctcgagttcc gccacactgg cgggcggatg cggagttcct tcgcggatca
     5341 gagcccgatt gaggacccca tcgcggtcca gaaagacggc gggatgcttc attttcccac
     5401 ggattcccat tttgtctgct tgggcttaag tcgcgggtga gaaaccagaa gatgccagat
     5461 caaagcctgg aatgcttcgg tgtgcggcgt aatggtctgc ggattgaccg tcggcacgat
     5521 cacacaagcg tccgccaccc gcgccgtatg tccaccgtcg cgccccacta caccgaaaac
     5581 actggcgcca accgccttgg cgtgatccgc agcagcgacc agattgcggc taatattctt
     5641 ctcggcgtca ccgccgccga cggagaatat gaacacggca tccttggcgc ccagcctgct
     5701 gattttaagc cactcgacca atgtgctctc ccagccgtca tcgttgattc gggcggtgag
     5761 ctctgcaaca ttgtcggtcg gcgcgtagca ctcgatccca acgagcttgc gaaaatcgtt
     5821 gaccgcatgc gagcaattgg cggcactgcc gcccacaccc aggaagaata tcctgcccgc
     5881 ggaagctttg atccgggcga gcgaatccac cagccgttct attgcggcaa cgtccagcct
     5941 ctgcagaatc gctactgctt cagccagata ttcctgcgtg tagctcatgc gttggccggt
     6001 cttcgtgaca ggaactcttg cgtctcgatg atcccctgct gcgagccaat ctcgtagaaa
     6061 cgacctgaga cttcgaacgc cgcaagctgt ccgctcgtca caagttcccg acagatgtcg
     6121 gccaggtcga tggatcgggc tggttgatac tttgcgaaga catccttcga aaacaccgat
     6181 aacccgaagt cgatgtgcgc catatcggcg cgtatcgagt gcttgtcgta ttcaacgatt
     6241 tcgccgcctc gatacacgat gttgctgcgg tcccagcgat tgtcattgcg taccagcgtc
     6301 atcaatcccg gacggccggc gctacggtag gcggattgaa tctgcgccaa cgagcacggc
     6361 agataggaat cgccgttcag aacaaagaaa tccgtaccga gcaggggcag ggccttcttg
     6421 atcgcaccac cagtccccag tagccgatca ccgtcgtacg aatagcggat tgtcaggccc
     6481 agcccattgc cgtcaccgac gctcgcccgg atctgatcgg cgaggtgacc gacgcacagc
     6541 acgacccggt cgataccctg gcttcgcagc agctcgagct ggtgatatat gaaaggccga
     6601 cccgcgaccg caatcaggga cttcggagtc ctcgacgtca ccggacccaa ccgagtcgcc
     6661 aacccgccgg ccagtatcgc aagcggcaac atgctcagga caggactacc ttggtgcctt
     6721 cgaaatcaaa gcgaaagcga acctcttcaa gtccggcctg gctcatcgcg taacgcaacc
     6781 gggctcgatc ggcggcgtag aacatcagga agcccccgcc tcccgcgcca accaacttgc
     6841 cgcctacagc gccgttcttc atagccagtg agtaccattg atcaatcttg aggttgctcg
     6901 ccgacccgca gcggcgcttc ttgtgctccc actgctcatg catcaactca ccgaatcgcc
     6961 ccagatcgcc ggactcgagg gcctccctgc tgcgatgccc caatgccttg acataatcca
     7021 gattccggat catgtcatcc tcccgcttga gcgatcgctc gtgctggtcc ttcaggatgc
     7081 tgcctgcggc cctgttagag ccggtgaaga agagcagcag attgtcttcc aggtcgtaca
     7141 tggtcttcgc gccgatgcgc aaaggctgga cagtgacccg atgatccttg tggaaggtga
     7201 ggcaggtgag cccgccaaag gcggcaatga attgatcctg cttgccgatc ggctcgccga
     7261 ggcgatcgat ttcgatgtga caggctagct ccgccaattc ctgcgagtgg gcgacccggc
     7321 gctggtacgc gtacagagcc ttcagcaacg ccgtggtgaa gctgccggag gagccgagcc
     7381 ccgttcccgc agggatgtcc gccagtgtcg taatttcgat ctgtggattc ggcatgtcct
     7441 gcatcgccaa tgcttcgcga atgatggggt gcttgacgtc cgcgatacgc tccacgtgct
     7501 ccaatgcgga atacttcaaa taaatccccg ggctgaacgg gcgcatgacc gtgacgtaga
     7561 cgtacttgtc gattgccgcc gcaatcagga atccttcacg ttcagcgtag taggaagcga
     7621 ggtcggtgcc gccgccgccc aacgtgatgc gaagcgggct acgcgcgata atcatagccg
     7681 ctgtccgcgt agattctagc caccaccgcc agcgccgcca tcgcatcttg caatcccgcg
     7741 gcgggctcgc gcccgctgcg cacatcctcg atgaactcct ccatttcgat cgaccatgag
     7801 tcgtcggcca ttgggtattc ccagatcgtg gtctcgggcg gccccatctg cggcagcatc
     7861 ttgtaccagg acagtcgctc catcccgtag ctgccaccga gtccatccac ctgcagcttg
     7921 ccgtcgcgac cgcagagttc ccacgagaaa atattcttcc actcggtgca actggcatgc
     7981 aggaaggcca cctgtttggt cgcggtcttc agcagcatga agccattgtc gtcaaccggc
     8041 atgttccaga aatacgtgtg ggcaaatccg tccacttcaa cgaactcgcc cagaaaccag
     8101 cgcgccagat caatcaggtg aacgccctgg tcgatcaact cgccaccgcc cgacctttgc
     8161 ggttgggcgc gccactcctg ctcatacccg actcgaccgc cgtgcccata tcgggcccgc
     8221 acgaacagca gcggccccaa agctccggcg tccaccagtc gtctggcctg tcgaaaggcg
     8281 cggtgatagc gatggttgaa gccgacgcgc acacgcacgt catgctcggc cgccaggcga
     8341 cgcagctcct gcaattcgtc gacatttcta cccgcaggct tctctaccag cacatgcttg
     8401 gccgcgctga tcgcggcgcg cacgatcggc gcctgcaagt ctgggggcgt tgccacgacg
     8461 acggcatcga tgtcaggacg tgccagcagc tcgcgccaat ccgcactgca agcgcacgcg
     8521 ggcgcgcgct gcgcgacccg ctgcgcttgc ggcaggtccg tgtcggcgca gtgaatcagg
     8581 ttcgcgcccg ccaggtgctg cgacctcctt tgtccgactg cgccacaacc gatgataccg
     8641 acgttcaatc gacctctccc caccccatct ggcggtcccg cggctgaatg cgacgcagcg
     8701 tatagatgtc ggtgcgattc agttcatcgc ggtgatccgg cagtatcctc caggggtccc
     8761 agaccgcact gatcaatttc ccggtgatgc cgtcactggc cgatgaaccg aggaatacgg
     8821 ccagttcggc ccccttgctc agtggtacgc cgccattgcg tttgtgctgc agtgaccgct
     8881 cgtagaattc ccgtcctatc ttgtcaggac cggcggcgat gaatccatcg aggaagcggg
     8941 tgtcgagaac acccggcgcg atcgcattga catcaatgtg gtaggagcgt gtctcttccg
     9001 cgagcgtctc catgaagcgc acgaccgcgg ctttggacgc cgcgtaggcg ctcagcatgg
     9061 gtagcgccgt ggtcgcgcct ccgcccgaga gttgtagaat tttcccgcgc ccggctttct
     9121 tgaaatgcgg caacacggcc cgactcagca gcacggggcc gagcaggttg atttcgatcg
     9181 cccgcgtcca ttcgcgccag tccacgctct ccaccgcgcc gctcggaccg gcgatgcccg
     9241 cattgcttac caggacatcg agccgaccga attcagacat cgtccgttcc accagcgtcg
     9301 tcacggcgtc cgggtcggag acatcggcgg tcatcgcaac gaccgcctga ttgacgcccg
     9361 caagcgctcg cagagtcgag caggcagcct gcagcggctc ctggccgcgc gcgcaaatca
     9421 tcagatttgc gccggccgcc agatagtgtc tggcgatttc cagacccaga ccttgactgg
     9481 ccccggtgat gatcgcgcat cgattctcaa gctgtctggt caccgtactc acggttcctt
     9541 caccggcatg ctcacgtcaa gataccgcag gtgggctggc agacccgctc ccggcgaaac
     9601 atacgccccg gccaggactt cccgatcctg cgcagatcgg atgcccacga cgactcggtc
     9661 aggatgcagg aagtcggcga ccgcccggcc caagacacgg atcgtcattg gcgtgcggcc
     9721 agcagccagg ggttggatct caggtactcc agggtggcga gcacgccctg ctgaattgag
     9781 cgctttggtt tccaacccag tgcgcgaatc ctatgagtgt caaggaagat gaatggactg
     9841 tcaccgaccc atccccggtc gccaccgcta tatacgatct cgggcctgag ccccaatgca
     9901 gcgcagatcc aacggatcga atcattgacc tcgcaaaagc catcgtgccc cagattgaac
     9961 acgttgactc gatcattcgc gcgctctatc gccagcagca tcgcgtcgat acaatcaccg
    10021 acgtacagat atgatttgcg ctgccggcca tttcccagca cgtatagtcg cccagggtcg
    10081 gccatcaact gccggtagaa atcgaacacg tggccgtgcg tatagcgctc gcccaggatc
    10141 gacaccaggc ggaaaatata ggcctgaaag ccgaatgcgg cgctgtatgc cgcgatcaaa
    10201 ccttctgcgg cagccttgga cgctccgtac aatgacgtct gaaccggaaa cggagcgtcc
    10261 tccggcgtcg gcgctatgga tgactcaccg tacacggaac cagaggaaga aaaggcgatt
    10321 cgatcgatgc cgttgcgccg cattgcctcc agcacattgt gagtggcaat ggtgttctgc
    10381 tccagatccc gacgtggatg ttcgacgccg aaccgcacgt cggcattcgc cgccaggtga
    10441 aatatgagct ccgatcccgc catgctgtcg ctaagagtct tcaagtccag gacatcagca
    10501 cgcaccagat gaaagttggc atgctgtcgc gccgagcgga cgaagtcctc ctgcccagtg
    10561 gaaaagttgt catagccaac cacggagtgc cccgttgcga gcagccgctg cgtcaaggta
    10621 ctaccaatga aaccggcaca accggtgacg agataccgca attgaccttc ctgcaccgcc
    10681 ccggggtttt atgacagggc acaccatcgt tggtaaatcg ttggtgcttc cacgtgttgc
    10741 cgcggtttta ctgtttgtcg cactggcggg gaactttgct gtgcacgcag tcgcgcccgc
    10801 gctgacccgc ctcgataccg actttcccga ctatcttacc gcggccagga tcgtagcaga
    10861 ccgaggggag gtggaccgcc tgtacgacaa cgcatggttc caggaccaga tgcgtcgcta
    10921 ccacattggc aagccctccg agggcaagtt cgcgccattt ccgccgccca cggcgctgct
    10981 gtgggtgtcg ctcgcgcggc taacgccgct cgatgcgctc agggtggtga cgggtctgag
    11041 cgttctggct ctggctgtct cggtcactct actgtcgcgt attctcggtt ggagcgtgct
    11101 tgacagcgca ctgctggtgt tgctttcggg ggtcgcagcc atcaacgccg tgcgacttgg
    11161 gcaaccctac atgctcgtgt cgctgtcctg catcctcggg tactacgcct acctccgggg
    11221 ccgcccgctg ttggcgggat tgtgtttcgg actgttcacg ccgatcaaat actttcccgt
    11281 agtcattctg atctacttcg cgttctgcaa gcagtggagg gtcgtgctcg ggggagcagc
    11341 cgcgatcctg gcggtcgtgc tggtcagcat cgggctgctg ggatggaagg ttcaccagga
    11401 attcttaagc tcggtgctcg gcatgcatct ggtgggccag ctgagcatgc aggatccatt
    11461 caccgccagt tttcagtcgt tcgatacgct gtttcgtcgg ttgttcgttt tcgatgcgac
    11521 atccaatccg catccgatgc tgtcgtcgcc tgaactcagc ggccctgcgg ttgcgatcac
    11581 gaagtccttg atcttctgca ccgcgatagc gacgctcatc cgcctggccc gcagcgcagg
    11641 ggagcgcgca ccgccagcgt cgatcggatt gctgggaatt ctggtattgc tgctcgcgcc
    11701 ggccaccgcc acctatcatt ttgtgctgct ctggctgccg atcggattgc tgatcaattt
    11761 cttgctgcgc gagggcgctc gcgcattcgc ctacttcatt ctgggtgtct acgcgctgat
    11821 cggattcttt ccgtaccgct tgagcgtcca ctttgaagga gatggagcgc tgaccctctt
    11881 ggcctatccg aggctttttc tactgctggc gatgttcatg gcctgtgtga ttttcgtctg
    11941 gcgtccgcgg cggggtatgg cgtgaagacg cggcagcaga acctggtttc gtatgcggcc
    12001 ttgctgctgt taggttcgct ggccttcatt cacctgatgg cgctgccgcc gttccaggac
    12061 gaaggaagtc agttgcgctg ggtgtggaga ctcatcaatg cgggggattg gcggccctcg
    12121 ttgagcgagg ggaaaccact ggaagcgtgg ctggttgcac cgctggcgct cgcgggtttc
    12181 gcaccactgg cggccgcgcg tacgctgcac gtattggccg gcatggcagg caccgggttg
    12241 atctatcagc tcgcgcttca gttcggcgac cgtacgcggg ccttcctgag cgcccttctg
    12301 ttcaccatct gtccgttcgt ggtctatctg cagcgcctgg ccctgtccga cattttcctg
    12361 tgcactgccg gcatctgggt gctgctgagc atcgcgcggc tgctcaagtc gcccacctgg
    12421 tcgcattctc tcgtgacggc catcgcactg ctgctggccg cgttgtgcaa actccctgtg
    12481 gggttcgtgt ttctgacttc aatgccgctg gcattgctgt tcatgcccag cagtgagcgc
    12541 caagtgcttc tgcgtcggcc tgagctgact aagctgcttg ccgcgtttgt tccggtactg
    12601 ctgctgctgc tgatcgtggc gtttacagcg atgatccagt tgcgacgtgg ccatgcgccc
    12661 ggctttggcg tggcggatct gatgggaatc ggcatggggc gctacgttga actggcgcga
    12721 gtacttggcg ttcagcgcag cagcctgctg gccgaactca cgacgcagat gtcatggcct
    12781 gtggtcatca ttggcctgat cggaatcgtc ggcggcgcgc tgagcggcga ttggcacgac
    12841 cgttggctca ttgctgcggg actgctgccg atgctcgcgg tcgcgtggct ggccgatttc
    12901 tggtttccgc gctacctatt gttcacgctg cctccactga tcgttgcagc tgtggccggc
    12961 tggcagtttt tgtcaacgcg tgcccggagc ctcgcgcgaa ctgtcgagct ggtcgccttg
    13021 acggcttgta tggtgttcat gggccaccaa tcggcattga tcatcctcaa tccgatcgcc
    13081 gcgagctggt cgcctgtgga ccgaactcag tacattgaag gcggcggctc cggttacggt
    13141 tacccgcagg cggcgaaata cctcctgcag gcgaacgacg tgccgcaggt catttactca
    13201 ctggatggac acagcgcgtt ccagctgctc acctatctgc cggccgcatg gaatagccgc
    13261 gtcaagacga tcagctatgc gcaagacggc caggagttgc gcagccagga agcgcgcttc
    13321 gagaacctgc tcgaacacgc accggcgtgg attgttgtcc cggatcccgt cctgacgagc
    13381 tatcttgaat ccagctttgg catgatgcgc ctgaaccgga tcaccttacg tccagtcgtc
    13441 cgcttcgaca aaccgggtgg tcacgaacaa ctggcgatat acgaagtgac gcggcgctag
    13501 ctggccagcc gagcatgtcg tcagtaatcc agtcggtaga ggctcggaac agtggagtcc
    13561 gttgccgtct cagagtatgg aaatctctcg atcaccgtca gcatcttgag gaactgttcg
    13621 ctgtcgtagc cgccaaggac acgcgctcgt cgctcttcga tgtaggccga ctcatcgagt
    13681 acgtagtgga tacgattccc gacgacgtag ctcgccagat cgttcgcctc gatgtacgga
    13741 tacacatcgt cgttcatgag gccgtccaaa ttgatcaaag gcggccgccc ggggcccgcg
    13801 ctgaagaagc tgagtattcc gcaactccag cagccgacgg tggcgcccgg cagttcctgc
    13861 ccgagacgca gcgccgcctt gtagtagagc aattgcatgt gaccgcgctc gataaaaccg
    13921 tcaatcaccg catagcccac cagagcgcac cagaagacgg cagccaccat ctgcatcgcc
    13981 gggcggcccg cgcgccaggc accctgtgag cacgccgcaa ccagcacgca aacgggcaat
    14041 gcgatgagac tggtgtacca actcagcacc gagggattca gtccgaacag aagcgagtag
    14101 atcaagagtg aacacaggct gagcgcgcac aggttggctt gttggagcgt caaagcctgc
    14161 agccatggga tcggcttgcg caatgccggt gccgctgccg ccgccagaat gagcacagcc
    14221 agcacggcca ataccggcgt gctgcggccg cgctgcggga tgccgatcgc gctcagcaat
    14281 aaatcgccga acggccgtaa gctaaagctg ctgcgcgtac cccacagcat cttgatccgc
    14341 acgctgccac tgagcagatg gccgctcaag gcataggtgt gcccgagcgt cagcaacaag
    14401 ccgagcgtgg cgccagccag cagcatgccg gcgccgtgca tggcaggtcg attgggccgc
    14461 cagcatgcca ccaggcccgt gccagccagg gtcgcggcag ctaaagcccc cgcgtcgctg
    14521 cgcgccagtt caagtagcag gccgagcgca agcaacccaa gcgcctgccg gcgcggcagt
    14581 ggcgcggagc caagccggga gaaaattgcg aagatgatgc tgctaatcag gacgacccag
    14641 ccccactcca taccggagga agcacccttc aggaagccca tagacagcag cggcagcgcc
    14701 agcgatgcgg ctgcgacctc gccgcacgtc cggcgtacga accgcacggt aacaacggcg
    14761 ccagccacgg cgcaggcggt ggacagcaca atggccaggt acaccagcct caagccgggc
    14821 ttcggcttca tgagccaact caaaccggcg agcgcgtaag cgtgtaacca ctgcacgccg
    14881 gtggtgtgcg ataggccgtg gtcgaacgtc cattcaccgg tgtcggcaaa attggtagca
    14941 gtgatgaagt agtaaaacgc atcgtccgga acgaagaaca cgatgcgccg cggtccgccg
    15001 ctaaggatcc acaccgctcg cgccacgatg accaggcaga cgaacgcagt ccaccacttc
    15061 accggcgccg tcagcgggct gcgcgatacc cggctcaagc ggctgagttt cacgatcaat
    15121 ggcctatgca gggctttgtg ccggctgacc ggcatatgca cggtcataat cgggtttcgc
    15181 ccggagcatc ggagcctcga tcagtcggta actgaaatga gcgatcgtga gcgacacaga
    15241 tgccatcaga cccgcatgca ggccgtagcc gccaggtatc caggatgcgc gccaaaacat
    15301 ccgcgtcatc aggaccatga caggtacatg ccagagatag gttgaatagc tgatggtgcc
    15361 agcatagcga agcggtcccc agccgagaaa ggctgtcata ccaggcgcgc ggcggaagat
    15421 gatggcgcca atcaggactg cagcgaaaat cgcaaaaacg cttacgccgg gaatggcatt
    15481 gagtcccaag gggccgccag gcgcacttgg cgaatacgaa tagagcgcca gaacagcggc
    15541 catgccaccg ccaggtatca ggagcgcgct aaaccaacgg cctgcacgag cgctgcttgc
    15601 tggttcggca tggctgcgcg cctccaccac ggcgaccagg gttccgagca caaaggcgtc
    15661 gagccgccct agcaacagaa acgtatagat tccctccgcg cgcagcaggt tgcaggcaat
    15721 caggaacagg gcgagcacgg cccaaggtag ccaccccgag cgcttcgtaa gccacgtgcc
    15781 tgccgcaaac atcagccagg caagcagcgg cgccagcaga tagaactgtt cctccagcgc
    15841 taacgaccag gcatgtccga acatgatcgg atagcccgga ccccacccgt tcgtgaacgg
    15901 ctgccagtaa aactcgctgt tctggacgaa gaacacacac tgcagcactc gccaactcaa
    15961 gctctcctcg ggcgcgtgga agtaatgaac atcgatccag gctgccgcgc acgcaaagcc
    16021 aagcactgca aagtacaacg gccagatccg caccacacgg cggcgcaggt acacccacca
    16081 gaagttgggt tggtgcctat gttgcaacga cccccgacca atgagatagc ccgacaacgc
    16141 aaagaacaaa tccatgcagc cccacaccca gggcaacgag gacccattca ggtggttgag
    16201 caccaccagc agcgacaaca agccgcgcag cccatcgagt tcgcgcactt tgccgatcga
    16261 cgatgctgct ctgtgggcag ctgtcgcgtc catgcgccaa taactacgct agctagtgcg
    16321 ccgcggtcgc ggtggccact gcgcgcgccg agtgcatgca atggtcactc ggctgcaccg
    16381 catcgagcgc tgcgcagatg tcgatactgt cgcccatctg tcgcggcacg ccggtacgtt
    16441 ccagcagcgc cgataacgcc ttcgccgaca tcgcgggtac ccgtgcacgc agcagcgcga
    16501 tcgcgccggt cacgtgcgct gcggcaaacg aagaacccgt gacgaagtca tagctgccac
    16561 cgggtgcggt gctgacgata tcgcgacccg gggcgtgcag gacgcccggc gcgtgttcgg
    16621 aacgtccgat ctgatcgacc gcaatcacgc cgccgacgtc gaggggaaac ccgtcgagcc
    16681 ggccgctttc gggtactgcg ccgacgaaga tcacaccgtg ctgcacaccg taactgacca
    16741 gctgcgacag cagcggatcg gcaggcccgc cgagactcag attgacgatc tgcgggtgcg
    16801 cgtcgatcgc ggcacccaag gcacgcgcca gagtgaagga attgcacacc gcttccatgc
    16861 tctgcgggtg ggccggttgg cacgccttgt agatcagcaa ccgcactccg ggggcaatgc
    16921 cgacaatacc caatccattg ttggcatcgg cgccaatgat gccggcgacc tcggtaccgt
    16981 gacgatcggt caggaacgcc ttcatgtctt cgtcgacaaa attgcggatg acggcgatgc
    17041 gtccgtccag atccggatgc gtggtcgcaa cgccggtatc gatgatggcg acgctgacgc
    17101 cgtcaccgcg cgaccattgc tgcgcagccg cagcgtcgat ttcgttgaag ccgtgctgca
    17161 gaccgtagta cggatcgttg tacgggctgc cgctgatagg tgccacgcgc agctccggcc
    17221 gcgctactga cgaattgccg acgtcgccat cggcgagcgc actgaacagt tgcagcggct
    17281 gggccagctt gacgcgaccg tcagcggaca gccgtcgcaa cagcgcgacg cgatcagcgc
    17341 tgggcggcac agcgaacacg aggcactgca ccttgagcgg caggatcggc cattcgcgca
    17401 cttcccgcag accgtagtcg tgcgccagtg cggcggtgac ggcgcgcgcc tggtcgctga
    17461 ccgtataggg gttggtggcg gaatagccgt ggggagtcga tccgggctcg ccgatcagcg
    17521 gacgcgtccg attgtccacg gtcacgacta tcagacgatc ggccgaggca ttcagtgcgc
    17581 catcatcgtg attgccgccc actgcgttgc gcggcgccgt ggcacacgat gacagtgcca
    17641 tcgcgcacag cagcgctgcc aggcgccaga gcgttgaata cccagtcacg cgcatcgtgc
    17701 tcatcaatct tagggcgaac ccccaatgac ttcagcaaa
//
LOCUS       DATMRM010000366         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1786700, whole genome shotgun sequence.
ACCESSION   DATMRM010000366 DATMRM010000000
VERSION     DATMRM010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1786700"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /gene="pyk"
                     /locus_tag="VNZ06_11105"
     CDS             <1..1366
                     /gene="pyk"
                     /locus_tag="VNZ06_11105"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWW21345.1"
                     /translation="MVQAGLDVARINFSHGTRDSQRKRVEMVRAAAKQAGRYIGLLAD
                     LAGPKIRIESFREGKVLLAEGASFALDTSLDPEAGSQTEVGVAYKNLTADVRSGDTLL
                     LSDGQIVLEVEKVVGTRIFNTVRSGGELSNRKGVNRQGGGISAPALTEKDLEDLRLAA
                     ELGIDYLAVSFARDADDMRRAQSELRKWRGEARVVAKIERHEALDNLAGILAVTDAVM
                     VARGDLGVEMGYAELTGLQKTIIRQSLARNRVVITATQMMESMIHAPIPTRAEVSDVA
                     NAVLDGTDAVMLSAETAAGKFPVKAVQAMAEVIRGAEKYQLGSPRAMQRAEEEFGNSE
                     EAIAMAVMYTANHMRVRAIVAMTESGMTPLWMSRIRADIPVYAFTRHEATRRRVTLYR
                     GVYAVPFDIVHTNTEALYSSIFDRLLELGLVDVNDLVILTKGELTGVSGGTNSMQILR
                     VVKR"
     gene            1434..>2313
                     /locus_tag="VNZ06_11110"
     CDS             1434..>2313
                     /locus_tag="VNZ06_11110"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWW21346.1"
                     /translation="MAAQITRSRAPIGSTVGLVLPGGGARAAYQVGVLKGIAEMFPPG
                     THNPFPIIVGTSAGAVSAAVLATEAYQWQGAVGSLERVWANFRVQQVFLVDTWNMMRS
                     GLHWLLSLISGGVLLPPPLALFDNSPLRSLLAHNIHWSELQRSIDGGDLEALALCATS
                     YGSASSVAFFQGGNKVTEWSRQQRIGRRTVLRLDHVMASLGVPFLFPPVQLGDEYFGD
                     GAQRQLWPLSPAIHLGADRLLVIGVRAEQGAGVNARPRTVVRPPTPGQLFGYMLDTLF
                     MDQIYANVEHVQRLNSV"
BASE COUNT          402 a          703 c          775 g          433 t
ORIGIN      
        1 catggtgcag gcgggcctgg acgtcgctcg catcaatttc tcgcacggca ctcgcgattc
       61 gcagcgcaag cgcgtcgaga tggtgcgcgc cgctgccaag caggcaggcc ggtacatcgg
      121 actgctggcg gatctggcgg gtccgaagat ccgcattgag agtttccgcg aaggcaaggt
      181 gctgcttgcc gaaggggcct cgtttgcgct cgacacctcg ctcgacccgg aggccgggag
      241 ccagaccgag gtcggcgtgg cttacaagaa tctgactgcc gatgtgcgct ccggcgatac
      301 gctgctgctg tccgatggac aaatcgtgct cgaggtcgag aaagtcgtcg gcactcgcat
      361 cttcaacacc gtgcgctcgg gcggagagct gtccaatcgc aagggtgtca atcgtcaggg
      421 cggcggcatt tccgcaccgg ccctgaccga gaaggacctc gaagacctcc ggttggccgc
      481 cgaactcggc atcgactatc tcgcggtgtc cttcgcgcgc gacgctgacg atatgcgccg
      541 agcgcagtcg gagctgcgca agtggcgcgg cgaggcgcgc gtagtcgcca agatcgaacg
      601 tcacgaggcg ctcgacaacc tggcgggcat tcttgccgta accgatgcgg tgatggtggc
      661 gcgcggcgat ctcggcgtgg aaatgggcta cgctgagctg accggactgc agaagaccat
      721 tattcgccag tcgctggcgc gcaatcgcgt cgtgatcacc gctacgcaga tgatggagtc
      781 gatgattcac gcaccgattc caacccgtgc ggaggtcagc gacgtcgcca atgcggtatt
      841 ggacggcacc gatgcagtca tgctctcggc cgaaaccgcc gccggcaagt ttccggtcaa
      901 ggcggtgcaa gcgatggccg aagtcatccg tggtgctgag aagtaccagc tcggctcccc
      961 gcgggcgatg cagcgcgccg aagaggagtt cggcaacagc gaagaggcga tcgcgatggc
     1021 cgtcatgtac acggccaatc acatgcgcgt gcgcgccatc gtggcaatga ccgaatcggg
     1081 catgacaccg ctgtggatgt cgcgcattcg cgccgacatt ccggtctacg ccttcacccg
     1141 gcacgaagcg acccggcgac gcgttacgct ctatcgcggc gtctatgcgg tgccgttcga
     1201 catcgtgcac accaacaccg aagcactcta cagctcgatc ttcgatcgcc tgctcgaact
     1261 ggggctggtg gatgtcaacg acctggtgat cctcaccaaa ggtgaactga ccggtgtctc
     1321 cggcggcacg aactcgatgc agatcctgcg ggtcgtcaaa cgctgagacg ccggtagcag
     1381 aatcggaacg atccgctatg ggtcgttgat ccagggtccg ctatgcttga cgcatggccg
     1441 cgcagataac caggtcccgc gcaccgatcg gctcgacggt cggactggta ttgcccggtg
     1501 gcggcgcgcg cgcggcctat caggtgggtg tgctcaaggg catcgcggag atgttcccgc
     1561 ccgggacgca caatccgttt cctatcatcg tgggcacctc ggccggcgca gtctcggccg
     1621 cggtgctcgc gaccgaggcc tatcagtggc agggggcggt aggctcgctc gagcgcgtgt
     1681 gggcgaattt ccgggtgcag caggtgtttc tggtggacac ctggaacatg atgagatcgg
     1741 gactgcattg gctgctgtcg ctgatctccg gcggtgtgtt gctgccgccg ccgctggcgc
     1801 tgttcgacaa ttcgccgctg cgctcgctgc tcgcgcacaa cattcactgg agcgagctgc
     1861 aacgcagtat cgatggcggt gatctggaag cgttggcgct gtgcgcgacc agctatggtt
     1921 cggcgtcatc ggtggcattt tttcaaggtg gcaacaaggt cacggaatgg tcgcgtcaac
     1981 agcgcatcgg ccgtcgtacc gtgttgcgac tcgatcacgt gatggcaagt ctcggcgtgc
     2041 cgttcctgtt tccaccggtg cagctcggcg acgagtactt cggcgacggc gcgcagcgcc
     2101 agctctggcc gctgagtccg gcgattcatc tgggggccga tcggttgctg gtgatcggcg
     2161 tgcgagccga gcaaggcgcc ggtgtcaatg cccgtccgcg caccgtggtg cgcccaccga
     2221 cgccgggtca gctcttcggt tacatgctcg acacgctttt catggatcag atctatgcca
     2281 atgtcgagca cgtgcagcgg ttgaacagtg tgg
//
LOCUS       DATMRM010000367         3520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3291718, whole genome shotgun sequence.
ACCESSION   DATMRM010000367 DATMRM010000000
VERSION     DATMRM010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3291718"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2236
                     /gene="ppc"
                     /locus_tag="VNZ06_11115"
     CDS             <1..2236
                     /gene="ppc"
                     /locus_tag="VNZ06_11115"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HWW21347.1"
                     /translation="IAWLMRLRDGGMLETGEGEVLEQAITRQIALLWQTRALRHERLY
                     VADEVDTALAYMREIFLPTIPALYARFERVLGSRPPSFLRLGSWIGGDRDGNPHVKAE
                     SLHLALGRASQTVLADYLSQLHLLGAELSISSELASVSEAVAGLAHRSGDVNPARADE
                     PYRRAITGIYARLAATYQRLFERPPARPASVTAAPYNDADSLRQDLLAVERSLLASPG
                     AALGQGGALTRLIRAVETFGFHLATLDLRQNADVHARVVGELLRVAGVEPSYAALEEA
                     ARVELLRRELQSERLLASPYATYSEETLSELAIVRAAARAHELYGSACITTYIISKCE
                     SVSDLLEVNILLKEAGLYRAHGATRAAIMAVPLFETIGDLKASPQIMRAWLSLPEVAA
                     VTGAKGYQEVMVGYSDSNKDGGYLTSVWSLHEATHTLAQVFEQFGTVMQIFHGRGGAV
                     GRGGGSSFAAIRAQPDGTVRGRIRITEQGEVIAAKYGTRENASANLEAITSATLLASL
                     EDASVPSALRVRFAESMEALSSSAFRAYRALVYETAGFSTVFRQMTPLREIAELKIGS
                     RPASRTNSDRIEDLRAIPWVFSWAQARVMLPGWFGVGQALSSFPDRSLLREMVAAWPF
                     FRATLDNLEMVLSKSDMSIAARYATLVQDQELGQQIFGRIRDAWSVTLEALLAVTGQS
                     RLLEKHPALDASVRLRLPYIEPLNWLQVELLKRHRVGENDLRVREGLQLSINAIATAL
                     RNSG"
     gene            2308..2454
                     /locus_tag="VNZ06_11120"
     CDS             2308..2454
                     /locus_tag="VNZ06_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21348.1"
                     /translation="MASIPIHPSVDHGVKAGSPGFAEPYDCLSPALMDLIATHTTKAK
                     GVLA"
     gene            2451..>3520
                     /locus_tag="VNZ06_11125"
     CDS             2451..>3520
                     /locus_tag="VNZ06_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013202337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HWW21349.1"
                     /translation="MKTRAAIAHAAGKPLEVVTLDLEGPKAGEVLVELKATGICHTDE
                     FTRSGADPEGLFPVIFGHEGAGIVVDIGQGVTSVKKGDHVIPLYTPECRQCKSCLSRK
                     TNLCTAIRATQGKGVMPDGTSRFSIGGQKVHHYMGCSTFSNFTVLPEIAVAKIREDAP
                     FDKVCYIGCGVTTGVGAVINTARVEPGANVVVFGLGGIGLNVIQGARLVGANRIVGVD
                     INPRRKALAEKFGMTHFVNPREAGADLVAHLVALTDGGADYSFECVGNVQLMRQALEC
                     CHRGWGVSVIIGVAGAGQEIQTRPFQLVTGRVWKGTAFGGARGRTDVPRIVDWYMDGK
                     ISIDPLITHKMPLERINDAFDL"
BASE COUNT          612 a         1048 c         1184 g          676 t
ORIGIN      
        1 catcgcctgg ctgatgcgac tgcgcgacgg cggcatgctc gagacgggcg aaggtgaagt
       61 gctcgaacag gccatcaccc gtcagattgc gttgctgtgg cagacccggg cgctgcgtca
      121 cgagcgtttg tatgtggcgg atgaggtcga taccgcgctt gcttatatgc gcgagatttt
      181 cctgccgacc atacccgcgc tgtatgcgcg cttcgagcgc gtgctcggca gccgtccgcc
      241 cagtttcctg cgcctgggga gctggatcgg aggcgatcgc gacggcaatc cgcatgtaaa
      301 agccgagtcc ttgcatctgg cgctcgggcg cgcctctcaa acggtgctgg ccgattattt
      361 gtcgcagctg catctgctcg gcgccgagct gtcgatctcg agcgagctgg cgagcgtttc
      421 ggaggcggtc gccgggctgg cgcaccgtag cggcgatgtg aatccggctc gtgccgatga
      481 accgtatcgc cgcgcgatca ccgggatcta tgcgcggctc gcggcaacct atcagcggct
      541 gtttgagcgc cctccggcac gtccggcctc ggtcacggcc gctccctaca acgatgccga
      601 cagtctgcgg caggacctgc tcgcggtcga gcgctcgctg ctggccagtc ccggggctgc
      661 tctgggccag ggcggggcct tgacgcgcct gattcgggcg gtcgagacct tcggttttca
      721 tctggcgacg ctcgatctgc ggcagaacgc cgatgtgcac gcgcgcgtgg tgggcgaact
      781 gctgcgggtg gctggagtcg agccgagcta tgcggcactg gaggaggctg cgcgggtcga
      841 gctgttgcgg cgtgagctgc aaagtgagcg cctgctggcc agtccctacg ctacctacag
      901 cgaggagaca ctatcggagc tggcgatcgt gcgtgccgcg gcacgggcgc acgagcttta
      961 tggcagcgcg tgtatcacga cctacatcat ctccaaatgc gagagcgtct cggatctgct
     1021 cgaagtcaat atcctgctga aagaggctgg cctgtaccgc gcgcacggtg caacccgtgc
     1081 agcgatcatg gcggtaccgc tattcgagac catcggggac ctgaaggctt cgccgcagat
     1141 catgcgtgcg tggctgtcgc tgccagaggt cgccgcggtc accggtgcca agggttatca
     1201 ggaagtcatg gtgggttact cggactccaa caaggacggt ggctatctga cttcggtgtg
     1261 gagtctgcac gaggcaaccc acaccctggc gcaggtattc gagcagttcg gtacggtgat
     1321 gcagattttc catggccgcg gtggtgctgt gggtcgcgga ggcggctcct cgttcgccgc
     1381 gatccgcgcg cagcctgacg ggacggtgcg cggtcgcatc cgcattaccg agcagggtga
     1441 ggtgatcgca gcgaagtacg gtacccgcga gaatgcatcc gcgaacctgg aagcgatcac
     1501 ctcggccacg ctgctggcgt cgctcgagga tgccagcgtg ccttcggcac tgcgggtgcg
     1561 tttcgctgaa tcgatggagg cgctctcgag cagcgccttt cgcgcgtacc gagcactggt
     1621 gtatgagacc gcggggttct cgaccgtctt ccggcaaatg acgccactgc gcgaaatcgc
     1681 cgagctgaag atcggctccc ggcccgcctc gcgtaccaat tccgatcgca tcgaggactt
     1741 gcgtgccatt ccctgggtat tcagctgggc gcaggcgcgc gtcatgctgc caggctggtt
     1801 cggagtgggt caggcgctca gcagcttccc tgacaggtcg ctgcttcggg aaatggttgc
     1861 cgcctggccg ttcttccgcg ccacgctcga caacctggaa atggtgctgt caaagtcgga
     1921 catgagtatc gcggcgcgct acgcgacgct ggtacaggat caggagctcg ggcagcagat
     1981 cttcggtcgt atccgtgacg cgtggtcggt gacgctcgag gctctgctgg cggtgaccgg
     2041 tcaatcgcga ctgctggaaa agcatccagc actcgacgcc tcggtgcgtc tgcgcctgcc
     2101 ttacatcgag ccgctcaact ggctgcaggt cgaactgctc aagcgccatc gggtcggtga
     2161 gaatgatctg cgggtgcgag agggactgca gctatcgatc aacgcgatcg cgaccgcact
     2221 gcgcaacagc ggctgacggc cgactggact atgatgccgt gccggcggcg cgcgtccctt
     2281 tcaacctaca aagcgaggca tccagccatg gcatccatcc ctattcaccc gtcggtcgat
     2341 cacggggtca aggcgggtag cccagggttt gctgagccgt acgactgcct ctcgccggcg
     2401 ctgatggatc tgattgccac ccacactacg aaggccaagg gcgtgctggc atgaagacac
     2461 gtgccgcgat tgcccatgcc gccggcaagc cgctggaagt cgtcacgctc gatctggagg
     2521 gaccgaaagc cggcgaggtt ctggttgagc tgaaggcgac cgggatctgc cacaccgacg
     2581 agttcacgcg ttccggcgcg gacccggaag gactgtttcc agtcatcttt ggacacgagg
     2641 gtgccggtat cgtcgtggat atcggtcagg gcgtgacctc ggtgaagaag ggcgatcacg
     2701 tgatcccgct ctacacgccg gagtgccggc agtgcaagag ctgcctgtca cgcaagacca
     2761 acctctgcac ggcgattcgc gccacccagg gcaaaggtgt gatgcctgac ggcaccagcc
     2821 ggttttcgat cggcggccag aaggtgcatc actacatggg atgttccacc ttctccaatt
     2881 tcaccgtgct gccggagatc gcggtcgcga agattcgcga agacgccccg ttcgacaagg
     2941 tttgttacat cggctgtggc gtgacgacag gcgtcggcgc agtcatcaat accgcgcggg
     3001 tggaacccgg tgccaatgtg gtcgtgttcg gtttgggggg catcgggctg aatgtcattc
     3061 agggtgccag gctggtcggc gccaatcgca tcgtcggcgt ggacatcaat cccaggcgca
     3121 aggcgctggc cgagaagttc ggcatgacac acttcgtcaa cccaagggag gccggcgcag
     3181 acctggtggc ccatttggta gcactcaccg atggtggtgc cgactacagt ttcgaatgcg
     3241 tgggcaatgt gcaactgatg cgtcaggcgc tcgaatgttg tcatcgcggc tggggtgttt
     3301 cggttatcat tggtgtcgcc ggggcagggc aagagataca aactaggccg ttccaactcg
     3361 tgacaggtcg ggtatggaag ggcacggcgt tcggtggggc gcgagggcgc accgacgttc
     3421 caaggattgt cgactggtat atggacggca agatttccat cgatccattg atcacgcaca
     3481 agatgccgct cgaacgcatc aacgacgcgt ttgatctcat
//
LOCUS       DATMRM010000368        13385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_192390, whole genome shotgun sequence.
ACCESSION   DATMRM010000368 DATMRM010000000
VERSION     DATMRM010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13385
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192390"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /gene="hutI"
                     /locus_tag="VNZ06_11130"
     CDS             <1..1002
                     /gene="hutI"
                     /locus_tag="VNZ06_11130"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HWW21350.1"
                     /translation="RSGEFEQRLRGASYQDILRAGGGILSTVRAVRAADATELTEQTI
                     ARAARLAALGVTTVEIKSGYGMSLADERKQLQAARSVGERVAMRVSKTFLGAHALPPE
                     FASRRSEFLAYMGGEVMPTLAAEGLIDAVDAFCESIAFSCDEVAGLFETAQALGLPVK
                     LHADQLSDGAGAELASRYRALSADHLEYSNARGIEFLARAGTVAVLLPAAFYFLREQQ
                     LPPVDQLRSAGVRIAIASDCNPGSAPLLSPTCVMNLACVQFRLAPHEALAGYTREAAR
                     ALGLLHETGTIEVGKSADFAIWRCSSPAELSYWIDGLAPVGRVFRGQPDPYGAGILQ"
     gene            999..1727
                     /gene="hutC"
                     /locus_tag="VNZ06_11135"
     CDS             999..1727
                     /gene="hutC"
                     /locus_tag="VNZ06_11135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HWW21351.1"
                     /translation="MSPPGNADDRPGALYEQVKRHIVERIRSGEWVSGARVPSENELV
                     QLLGVSRMTVHRALRELSSQGLLARVQGVGTFVAPAHAKSELLEVRDIGDDVVARGHE
                     HSVKVVAQDAVRASPDMAVMFDLRPGAKVFHSLLVHFEDELPIQQEERFVRPSFAPSY
                     LEQDFTRQTPGNYLLSIALPTELDHVVFAVLPDKRAQRLLKVGATEPCLMLTRRTWVT
                     AVPVTRSSFIYPGSRHSLGSRHKL"
     gene            1775..2356
                     /locus_tag="VNZ06_11140"
     CDS             1775..2356
                     /locus_tag="VNZ06_11140"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HWW21352.1"
                     /translation="MSQTKRILVLGLGVAGLAAAALLVHAAQPALNIKLGLWETHTEA
                     KLSGDMSAMIPEDQLAKMTPEQKARIQAAMQQSMAAMQKPHYAKECMTAEKVAKGFDM
                     GDEKSGNCKNTISTNTGSQYEARVVCTGNYGQQAMTVHITADSPTHVIGTVKGDESQG
                     SKGMSYSGTFEGRFLGSDCGAIKDRQEEPGPSK"
     gene            2450..3283
                     /locus_tag="VNZ06_11145"
     CDS             2450..3283
                     /locus_tag="VNZ06_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HWW21353.1"
                     /translation="MSDAPSQLSKEPWYLPVGDEVQMFEAAYRSRLPVLLKGPTGCGK
                     TRFVEYMAWRLYRQPGSPHRNIERPLVTVACHEDLTATDLVGRYLLSGDATVWMDGPL
                     TSAVRTGAICYLDEVVEARKDTTVVVHSLTDHRRVLPIEKTGELLDAHPDFLLVMSYN
                     PGYQSVLKDLKPSTRQRFVSLEFNYPEIEVETRIIAHEGGIDAQLAQRLALIGQKVRN
                     LREHGFEEGVSTRLLVYAAQLVARDISPRRACDVAVASAVTDDPDVRQAISSIVDVLL
                     P"
     gene            3280..5268
                     /locus_tag="VNZ06_11150"
     CDS             3280..5268
                     /locus_tag="VNZ06_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWW21354.1"
                     /translation="MNPDQRVLLADIQRPIALFAQGLVGHFVHLRPTSRNAPESRSSD
                     GMGTAGDGIELPAQIAEFDTRVHNLGAYRVTVLRQIGYLTDGPAHFSLARAAERLAIP
                     FAVSAAPKLSELEAFLALGSRPVLLRTVFLTLESRRIDGLIAQRYPGAGGDLARLRAR
                     ALATRPQTRAGNALTGVVEALVRYTLGGERAGLADVLLALADAAIAPGADVYATASAA
                     VAFCRALDPRFRLRAPLSGAIGTQSLEPDAQAAEYLPAPEDLASLGIDFPGEMILQTL
                     NARRRRGGQIAGPQSPSSPVLTSGMPDTATRDLKAADVGARAAASSTQSRESARSYHY
                     DEWDYHAQQYLRAWCRLYEQRLRGERFDFIDDVHRRHALLARQVRRQFGFIRPEQWHR
                     VRRTNDGDELELDGVIEAVIDRRLAGASDSQLYIRRDRALRDVAAAFLMDMSASTGFP
                     LPEASTPAAATHAPAAQYAPYFYGGLDDVAVSKPPPKRRVIDVARDALALMCEALQRL
                     GDSCAIYGFSGDGREQVEFLVAKDFRDRLSAQTWAALAAMEPLRSTRMGPAIRHALMK
                     LLREPARMKVLIIVSDGYPQDHDYGPDRRDDSYGIEDTACALREAERAGVIAFCVTID
                     PAGHDYLRRMCADSRYMVIDDVMALPRELTKIYRTLTA"
     gene            5336..6793
                     /locus_tag="VNZ06_11155"
     CDS             5336..6793
                     /locus_tag="VNZ06_11155"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HWW21355.1"
                     /translation="MKEISLLKSQNYIDGQWVAADSGAVVEVRNPANGERLTSVPNSG
                     SAETRRAIEAARRSFPEWKARTAEDRGRILRRWFELMMTHQEDLARLMTSEQGKPLTE
                     SRGEIAYAASFIEWFAEEGRRLYGDVIPSPWRDKRIVVTREPIGVCAAITPWNFPAAM
                     ITRKVGPALAAGCTMVVKPALQTPLSALAMAELAARAGVPAGVFSVVTGSSRAIGAEL
                     TGNPIVRKLSFTGSTEVGRLLTAQCAPTLKHLSLELGGNAPFIVFDDADLDAAVEGAI
                     TSKYRNTGQTCVCANRLFVQDSVYEAFSRKLVAAVSRLKVGNGIDSGIEQGPLIDQAA
                     LSKVEQLVGDAVGQGARALVGGHRHSLGGTYYEPTVLVDVTPTMQIAHEEIFGPVAPL
                     FRFATEADAICMANDTEFGLAAYFYARDVGRVWRVSSALECGIIGINTGLISTAVAPF
                     GGMKQSGIGREGSRYGIDEYLEMKYLCMGGTAQPG"
     gene            complement(6976..8073)
                     /gene="ald"
                     /locus_tag="VNZ06_11160"
     CDS             complement(6976..8073)
                     /gene="ald"
                     /locus_tag="VNZ06_11160"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HWW21356.1"
                     /translation="MRIGVPKEIKVHEYRVGLVPAAVRELTSAGHEVLVQSGAGGGIG
                     CSDEDYRSAGARIAPDADAVFKDSQMIVKVKEPQLTECSRLRRDQVLFTYLHLAADRA
                     QAEGLIKSQCVAIAYETVTASDGSLPLLTPMSEVAGRMAIQVGAQYLLQANGGRGILL
                     GGVPGVAPAKVVVIGAGVAGTHAIEMAHGLRANVIAIDRSIKRLRELDVQFRGSVRTL
                     YSTAEAIETFVADADLVVGAVLLPGAAAPKLVNGAMIKRMRPGSVVVDVAIDQGGCFE
                     TSRPTTHAAPTYVVDGVIHYCVTNMPGAVPRTSAFALNNATLPYVRALADKGWERATA
                     DDRGLAAGLNIVRGSVVHPAVAKELGLPAAA"
     gene            8224..8691
                     /locus_tag="VNZ06_11165"
     CDS             8224..8691
                     /locus_tag="VNZ06_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWW21357.1"
                     /translation="MQLDRTDRKILRELQRDARQSNSALAAGVGLSESACLRRVRLLE
                     QAGVIERYTAMLNAARIGVSVGFIIRITLKGQTDRDLGAFEQAVQAVPEVTECDLTTG
                     ESDYVLRVVARDASDFERLHSKVLTKLPGVARVDSSFVLRSVVKNAGLPIDTQ"
     gene            8741..9391
                     /gene="maiA"
                     /locus_tag="VNZ06_11170"
     CDS             8741..9391
                     /gene="maiA"
                     /locus_tag="VNZ06_11170"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HWW21358.1"
                     /translation="MLKLYTYFRSSAAFRVRIALNLKGLPYESIPVHLLRGAGEQHSE
                     SYATRNAARLVPTLADGALSLGQSLAIMEYLEETHPAPALLPADALTRAKVRELALSV
                     ACDIHPLNNLRVLRYLHAPLQIEEERRNEWARHWISLGFTAIEHTLERSGALSTGYCF
                     GTQPTLADCCLIPQVFNARRVSLPMDEYPSIQQVHDHCMQLPAFIKASPGAQSDAE"
     gene            9381..9839
                     /locus_tag="VNZ06_11175"
     CDS             9381..9839
                     /locus_tag="VNZ06_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HWW21359.1"
                     /translation="MPNRQRRLRLQDYLPYRLSVATNAVSRLIARAYESRFALKIPQW
                     RLIAVLADEGPLTQQALCVRTIMDKVTVMRAAQGLLERGLIRRLPNAQDRRSHRLIMT
                     AAGQRLYDQIVPMALEYQARLLHGMDRADILQLEQLLRRLEQSAIALSGG"
     gene            9940..11361
                     /locus_tag="VNZ06_11180"
     CDS             9940..11361
                     /locus_tag="VNZ06_11180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWW21360.1"
                     /translation="MTAALSARIGRACVVAIAFLTLDGCSVGPIYRHPEMPLPSAWRN
                     AAPVPQDWPRVDWWQDFGSAQLNEFMTQARAGNDDIAAAIARVRQADAQVRIAGSPLL
                     PTIDLGASAARQRTLSVSGGRTPIYNEFSPQLSASYELDFWGKNRAVRSAAVAAAQAS
                     RYDRDTIALSVYTSVALTYFQALEAHDRLVVAQENLANAQHILEGLQLEQQVGIATAL
                     DVAQQATTVATVNATLPPLALQLQQNIDALAILAGQSPESVKLTIESGLDDLTPPPVI
                     AGMPSELLTRRPDVAQAEAQLRAANADIVAARAAFFPNIELTASGGFASTVLAGLLRP
                     GNEVFSVAASATQTIFDFGARSGQLAFSRARYTELLSSYHKTVLTAFGNVEDALVAVQ
                     QTADEQVRQQDAVDKAGRAFEFAQLQMQAGTTNVLTELNTEIALFTAKDALVQVKFLH
                     LQALVDLYQALGGGWHQAQEPTT"
     gene            11358..12509
                     /locus_tag="VNZ06_11185"
     CDS             11358..12509
                     /locus_tag="VNZ06_11185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWW21361.1"
                     /translation="MKIRGWLLVGAVLLAAGWLWLTRKSADAAGPKRAAAAVAVDSAL
                     AVRHDVPVYLEGLGNVQAFYTAKISSRVDGQLDRVGFVEGQLVKKGDLLAQIDPRPLQ
                     AALGQADATQAKDAAQLESARRDLERYAVLAPKNLVSQQILDAQKALVAQLEAQVKVD
                     GAAIDNARTQLQYASITAPFSGRTGIRLVDPGNIVHATDTTGIVVLTQIQPISVVFTL
                     PEDAISQVTQAISAGKVTVTAMSRDDRTQLDSGTLALVDNQIDMTTGTVKLKATFDNT
                     RNALWPGQFVNVRILLQQRHDAITVPTPAVQRGPDGMFAYVVKADLTVEPRPIETAEE
                     ADGLTVITGGLAAGERVVTTNQYRLQPGASVQLLAPTSTQSVETSAPTS"
     gene            12506..>13385
                     /locus_tag="VNZ06_11190"
     CDS             12506..>13385
                     /locus_tag="VNZ06_11190"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW21362.1"
                     /translation="MSISEPFVRRPIATSLLMSGILLVGIVAWPLLPVAPLPQVDFPT
                     VVVTGQLPGASPETMASSVATPLEYQFAQIPGLTQMTSTSVLGSSQVTLQFDLDRPID
                     AATEDVQLAIQAAGGQLPKNLPNPPTIRKINPADSPIVILSVHSDVLPLTSVDDYCEN
                     VIAQQISQIPGVAQVTIGGQQKPAVRVQVDPAKIATLGLQLEDIAGVIAAATVDTPKG
                     SINGPLHSFAIYDNDQLLKAAPWNDVVVAYKNGAPIRIRDIGVAVDGPENNQIVGWQN
                     NHYGIQLQIFKQPGANV"
BASE COUNT         2407 a         4210 c         4372 g         2396 t
ORIGIN      
        1 cgcagcggcg agttcgagca gcgcctgcgc ggcgcgagct atcaggacat tcttcgggcc
       61 ggcggcggca tcctctccac ggtgcgcgcg gtgcgtgcgg cggatgcgac cgaactcacc
      121 gagcagacca ttgcgcgtgc ggcgcgcctg gccgcgctcg gcgtgacgac ggtcgagatc
      181 aagtccggct atggcatgag tctggcggat gaacgcaaac agctgcaggc tgctcgcagc
      241 gtcggtgaac gggttgcgat gcgcgtcagc aagaccttcc ttggggcgca cgcgttgcct
      301 ccggagtttg cctcacggcg ctccgagttc cttgcctata tgggcgggga ggtcatgccg
      361 acgctggcgg ccgaaggcct gatcgacgcg gtggacgcgt tctgcgagag catcgccttt
      421 tcatgcgatg aggtggcggg tctgttcgag accgcgcagg cgctgggact gccggtgaag
      481 ctgcatgcgg accagctctc cgatggcgcc ggcgcggaac tcgcatcgcg ctaccgtgcc
      541 ctgtcggccg atcatctcga gtacagcaat gcgcgcggca tcgaatttct ggcgcgggcg
      601 gggaccgttg cggtgttgct gccagccgcg ttctatttcc tgcgcgagca gcaactcccg
      661 ccggtcgatc agttgcgctc cgcgggcgtc aggatcgcga tcgccagcga ctgcaatccg
      721 ggctcggcgc cgctgctcag tccgacctgt gtcatgaacc tggcgtgtgt ccagttccgc
      781 ctcgccccgc atgaggcgct ggccggctat acgcgtgaag cggctcgcgc gctcggctta
      841 ctgcacgaga cgggtaccat tgaagtcggc aagagcgccg actttgcgat ctggcgctgc
      901 tcgagccctg ccgaactgag ctactggatc gacggactgg cccccgtcgg gcgtgtgttc
      961 cgtggtcagc ccgatccgta cggcgcgggc atcctgcaat gagtccgccg ggcaatgctg
     1021 atgacaggcc aggggccctc tacgagcagg tgaagcgtca cattgtcgag cggatccgca
     1081 gcggtgaatg ggtcagtggc gcgcgcgtgc cttccgagaa tgaactggtg cagctgctcg
     1141 gcgtttcgcg catgaccgtg catcgggcgc tgcgagagct gtcgtcgcag ggattgttgg
     1201 cacgcgtgca gggcgtgggc accttcgtcg cacccgcaca cgccaaatcc gagctgctcg
     1261 aagtacgcga catcggtgac gatgtggtcg cgcgcggcca tgagcacagc gtcaaggtcg
     1321 tcgcgcagga tgccgtgcgc gccagtccgg atatggcggt gatgttcgat ttgcggcccg
     1381 gcgccaaggt gtttcattcc ttgctggtgc atttcgagga cgaattgccg attcaacagg
     1441 aagagcgctt cgtccggccg tcgttcgccc cgtcctatct ggagcaggac ttcacgcgcc
     1501 agactccggg aaactacttg ctgagcatcg cgcttcccac cgagcttgat cacgtggtat
     1561 tcgcggtgct gccggacaag cgtgcgcaaa gattactcaa ggtcggggcc acggagccgt
     1621 gcctgatgct gacacggcgt acctgggtga ccgcagtgcc ggtgacccgc agcagcttca
     1681 tctatccagg ttcgcgccac agccttggca gcaggcataa actatgatcc tgcgcagtca
     1741 tatcaaatca cgccattgac tttaggaggg tgagatgtct caaacgaaac gcatcctggt
     1801 tctgggtctc ggggtcgccg ggttggccgc ggccgcgctg ctggtacacg ccgcacaacc
     1861 ggcgctcaac atcaaactgg gactttggga aacgcacacc gaagcgaaac tgtcgggcga
     1921 tatgtcagcg atgattcccg aagaccagct ggcgaagatg acaccggagc agaaggcgag
     1981 aatccaagca gcgatgcagc agtcgatggc ggcgatgcag aagccgcact atgcaaagga
     2041 gtgcatgacc gcggaaaaag tggccaaggg tttcgacatg ggtgatgaga agtccggtaa
     2101 ctgcaagaat acgatcagca cgaacaccgg atctcaatac gaggcgcgcg tggtgtgcac
     2161 gggcaactat ggccagcagg cgatgacggt tcatattacg gccgacagtc cgacccacgt
     2221 gatcggcacc gtcaagggcg atgagagcca gggatccaag gggatgagct acagcggtac
     2281 cttcgagggc cgatttctcg gctccgactg cggagccatc aaggaccgac aggaagagcc
     2341 cggtccgtcg aaataatggc gtagacgctt cgcagcagcg gtggccgtgc gcggtcaccg
     2401 ctgctcaaac ccgtttcctt cataggcgat ctgcggcatc ctgctgcgtg tgagcgatgc
     2461 ccccagccaa ttgtcgaagg agccgtggta tctgcccgtc ggcgacgaag tgcagatgtt
     2521 cgaagcggcc tatcgctcgc gattgccggt gctgctgaaa ggccccaccg gctgcggcaa
     2581 gacgcgcttc gttgagtaca tggcgtggcg gctgtaccgg caacctggca gtccacatcg
     2641 aaacatcgag cgcccgctgg tgacggtcgc ctgtcacgag gatctgacgg cgaccgatct
     2701 ggtcgggcgc tatctgttgt cgggcgatgc gaccgtttgg atggacggac cgctcacttc
     2761 ggcggtgcgc accggcgcca tctgctatct agacgaggtc gtggaggcac gcaaggacac
     2821 gaccgtggtg gtgcattcac tgactgatca ccgcagggtg ctgccgatcg agaagaccgg
     2881 cgagctgctg gatgcgcatc cggacttcct gctggtgatg tcgtacaacc cgggctacca
     2941 gagcgtgttg aaggacctga aaccctccac acgccagcgc ttcgtgagtc tggagttcaa
     3001 ctaccccgag attgaagtcg aaacccgcat catcgcgcac gaaggcggca ttgacgcgca
     3061 gctcgctcag cgcctggcgc tgatcggtca gaaggtgcgc aacctgcgtg agcacggctt
     3121 tgaagaaggc gtcagtacac gcctgctggt atacgccgcg cagctggttg cacgcgacat
     3181 atccccgcgg cgcgcgtgcg atgtcgcggt ggcgagcgcc gtcacggacg atccggacgt
     3241 gcgacaggcg atcagctcaa tcgttgacgt tctgttgccg tgaaccctga ccagcgcgtc
     3301 ctgctggccg atattcaacg tccgattgca ctgtttgctc agggcctggt ggggcatttc
     3361 gtgcacctgc ggccaaccag ccgcaacgca cccgagagcc gctcctcgga tggcatggga
     3421 acagcagggg acggcattga gctacccgcg cagattgccg agttcgacac gcgcgtacac
     3481 aacctcggtg cctaccgggt cacggttctg cgccagattg gctatctgac cgacggtccc
     3541 gcgcacttca gcctcgcacg ggccgccgag cggctcgcga tacccttcgc agtgagcgct
     3601 gctccgaaac tgtccgagct ggaggcattt cttgcgctcg gatcgcggcc ggtactgctg
     3661 cgtacggttt ttctgacgct ggagagccgg cggatcgatg gcctgatcgc gcagcgctat
     3721 cccggtgcgg gcggcgatct tgcgcgcctg cgcgcccgag cgctggccac tcgcccgcag
     3781 acgcgcgcag gcaacgcgct gaccggcgtc gtggaggcac tggtgcgcta cacgctcggg
     3841 ggtgagcgtg cagggctagc cgacgtgctg ctggcgctgg ccgatgcggc gatcgcgccc
     3901 ggtgctgatg tgtacgcgac cgcgagcgcg gccgtggcgt tctgccgcgc gctcgatccg
     3961 cgctttcggc tgcgtgcgcc gctatccggt gcgataggca cgcaatcact tgagcctgac
     4021 gcgcaggcgg ctgagtactt gccagcgccc gaggatctgg cgagtctggg aatcgacttc
     4081 cccggcgaga tgattcttca aacgctgaac gctcgccgcc gccgcggtgg ccagatcgcg
     4141 ggcccgcagt ccccctcatc cccggtcctg acttccggca tgccggacac cgcgacccgg
     4201 gatctgaagg ctgctgacgt gggcgcacgc gcggcggcgt catcgacgca gtcgcgtgaa
     4261 tccgcgcgca gctatcacta cgatgaatgg gactatcacg cacagcaata cctgcgtgcg
     4321 tggtgtcgcc tgtacgaaca acgactgcgc ggcgagcggt tcgatttcat cgacgacgta
     4381 caccgtcgac atgcgctgct cgcgcggcag gtgcgccgtc agttcggctt catccggccc
     4441 gagcagtggc atcgggtgcg ccgcaccaat gacggcgatg agctggaact ggacggcgtg
     4501 atcgaagcgg tcatcgatcg acgcctggcg ggggccagcg actcacagct ctacattcga
     4561 cgcgaccgtg cactgcgcga cgtggccgcg gcgttcctga tggacatgag cgcctcgacc
     4621 ggattcccgc tgccggaagc ctcgacaccc gctgcggcaa cccacgcacc cgccgcgcag
     4681 tatgcgccgt acttctacgg ggggctcgac gacgtagcgg tgagcaagcc gccgcccaag
     4741 cgtcgggtca tcgatgtggc cagggatgcg ttggcgctca tgtgcgaggc attgcagcgg
     4801 ctcggggaca gttgcgccat ctacggtttt tccggagacg gccgtgagca ggtcgagttt
     4861 ctcgttgcca aggacttccg tgatcggctt tcggcccaga cgtgggcggc gctggcagcg
     4921 atggagccgc tacgctcgac gcgcatgggc cccgccatcc gccacgcgct gatgaaattg
     4981 ctgcgcgagc cggcgcgaat gaaagtgcta atcatcgtct cggatggcta tccgcaggac
     5041 cacgactatg gtccggatcg cagggacgac agctacggaa ttgaagacac ggcctgcgca
     5101 ttgcgggaag ccgagcgcgc cggcgtgatc gctttttgcg tgacgatcga tcctgccggt
     5161 cacgactacc tgcgtcgcat gtgcgctgac agccgctaca tggtcatcga tgatgtcatg
     5221 gcgctgccgc gcgagctgac caagatctac cgcacgctga cggcctgagg cacagctgcc
     5281 gacgccgacg catcgctacg aaccacaaac agagttaatc ttggaaccta tgcaattgaa
     5341 agaaatcagt ctgctgaaga gccagaacta catcgatggc caatgggtcg ccgctgacag
     5401 cggcgcggta gtcgaggtgc gcaaccccgc caatggcgag cgcctgacca gtgttccgaa
     5461 ctccggcagc gcggagacgc ggcgggcgat cgaggcggcg cgacgctcct ttccggagtg
     5521 gaaggcccgc accgctgagg atcgtgggcg catcctgcgc cgttggttcg agctgatgat
     5581 gacgcatcag gaggatctcg cgcgcctgat gaccagcgag cagggcaaac cgctgaccga
     5641 atcgcgaggc gaaatcgcgt atgcggcatc tttcatcgag tggtttgccg aagaggggcg
     5701 ccgcctctat ggggatgtca tcccttctcc ctggcgagac aagcgcattg tggtcacacg
     5761 cgagccgatt ggcgtttgtg cggcgatcac gccgtggaat ttcccggcgg cgatgatcac
     5821 ccgcaaagtg ggacccgcgc tggctgccgg gtgcacgatg gtggtcaagc cggcgttgca
     5881 gaccccgctg tcggcgctgg cgatggccga gctggcagcg cgcgccggcg tgccggcggg
     5941 cgtgttcagc gttgtcaccg gcagttcgcg tgcaatcggt gcggagctca ccggcaatcc
     6001 gatcgtccgc aaactcagct ttaccggctc gaccgaagtt ggtcgcctgc tgaccgcgca
     6061 gtgcgctcca acactcaaac atctgtcgct ggaactcggc ggcaatgcgc cgttcatcgt
     6121 gttcgacgat gccgatctgg acgcggcggt ggaaggcgcg atcacttcca agtaccgcaa
     6181 taccgggcag acctgtgtgt gcgcgaatcg cctgttcgtc caggactcag tctacgaggc
     6241 cttctcgcgc aagctcgtcg ccgcggtgag caggctcaag gtcgggaacg gcatcgacag
     6301 cgggatcgag caggggccgc tgatcgacca ggccgcgctg tcgaaggtcg agcaactggt
     6361 cggcgacgcg gtcggtcagg gcgcccgagc cctggtgggt ggtcatcggc acagtctcgg
     6421 cggcacctac tatgaaccca cggtgctggt ggacgtgaca ccgacgatgc agatcgccca
     6481 tgaggaaata ttcggacctg ttgcgccgtt gtttcgcttc gcgaccgagg cagacgcgat
     6541 ttgtatggcg aacgacacag agtttggact ggctgcctac ttttacgcgc gcgatgtcgg
     6601 ccgggtgtgg cgcgtatcga gcgcactcga gtgcggcatc atcggcatca acaccggttt
     6661 gatcagcact gcggtcgcgc cgttcggagg aatgaagcag tcgggtatcg gtcgggaagg
     6721 ttctcgctac ggaatcgatg agtatctcga gatgaaatat ttgtgcatgg gtggaacggc
     6781 gcagccgggt tgacgctata atctatggac ttgtcagcac ggacccatct cgtgatgaga
     6841 cgtgatgtga cgcagagcgg cgacgacggc cttgttgttt gattcccgat cggcccggag
     6901 gtgctccccc gcatccctgc gctgagtttg caaggaagcg atcgccgctc ttttccttac
     6961 ctattaatta ctgaatcacg ccgccgctgg cagtccgagt tccttcgcga ctgccggatg
     7021 cacgacgctg ccgcgcacga tgttgagtcc ggccgccaat ccgcgatcat cggctgtcgc
     7081 acgttcccag cccttgtcgg ccagcgctcg cacgtagggc aacgtcgcat tgttgagagc
     7141 gaatgcggag gtgcgcggca ccgcgccggg catgttggtg acgcagtagt ggatgacgcc
     7201 gtccaccaca taggtaggtg ccgcatgcgt ggtcgggcga ctggtttcaa agcatccgcc
     7261 ctgatcgatc gcgacgtcca ccacgactga gcccggacgc atgcgcttga tcatcgcgcc
     7321 gttgacgagc ttcggtgccg ccgcgcccgg cagcaacacc gcaccgacca ccagatcggc
     7381 gtcggcaaca aatgtctcaa tcgcctccgc cgtcgagtac aaggtccgca ccgaaccgcg
     7441 gaactgaaca tcgagctcgc gcagccgttt gatggaacgg tcgatcgcga tgacattggc
     7501 gcgcaggcca tgcgccattt cgattgcatg agtaccggcg acgccggcgc cgatcacgac
     7561 cactttcgcc ggcgccacac cggggacccc gccgagcagg attccgcgac cgccgttggc
     7621 ctgcagcaga tactgcgcgc cgacctggat tgccatgcgc ccggcgactt cgctcatcgg
     7681 cgtcagcagc ggcagtgagc cgtcgctggc cgtgacggtc tcgtaagcga tcgccacgca
     7741 ctgcgacttg atcaggcctt cagcctgggc gcgatcggcc gccagatgca gataggtgaa
     7801 cagcacctga tcgcgccgca gccggctgca ctcggtaagc tgaggctcct tgaccttgac
     7861 gatcatctgc gaatccttga ataccgcatc ggcatccggg gcgatcctcg ctccggcact
     7921 gcgatagtcc tcatcgctgc atccaatgcc gccaccggcg ccactctgca ccagcacctc
     7981 gtgcccggcg ctggtgagtt cgcgcaccgc cgcggggacc aggccgaccc gatactcgtg
     8041 aaccttgatc tctttgggaa cgccgatgcg catcgctgtc tccgtggtgg ccgcgggcta
     8101 agtaccgcgt agatgaatcc ggtaattgtc gcgctcacgg gctcgcaggc gattgcaaaa
     8161 ttcgccctcc cagccgctat gggcggcaga ttcttgcgaa aaactgtcat ataatagctg
     8221 aatatgcagc tggatcgcac agaccgcaag attctccgcg agctgcagcg ggatgcgagg
     8281 cagtcgaatt cagcgctcgc cgcgggggtc gggctttccg agtcggcctg tctgcgccgt
     8341 gtgcggctac tggagcaggc cggcgtcatc gagcgctaca ccgcgatgct gaacgcggcg
     8401 cgtatcggtg tcagcgtggg gttcatcatt cggatcacgc tcaagggtca gaccgatcgt
     8461 gatctgggtg cattcgaaca ggcggtgcag gcagtgccgg aagtgaccga atgcgatctg
     8521 accaccggcg agtcggacta cgtgctgcgg gtggtggcgc gcgatgcgtc cgatttcgaa
     8581 cggctgcaca gtaaggtgct caccaagctg ccgggagtcg cgcgcgtgga ttccagcttc
     8641 gtgctgcgat cggtggtcaa gaacgccggc ctgccgatcg acacccaata gttacagatg
     8701 caaccactgt atgattgctc agcgaccagg aagaaccgac atgctcaagc tttacactta
     8761 tttccgcagt tccgcggcct ttcgcgtcag gattgctctc aatctgaagg gcctgccgta
     8821 cgagtcgatt ccggtacatc tgctgcgcgg agccggtgaa caacacagcg agagctacgc
     8881 gacgcgcaat gcggcgcgct tggtgccgac gctggcggat ggcgcgctga gcctcggcca
     8941 gtcgctggcc atcatggaat acctggaaga gacgcacccg gcgccggcgc tgctgcccgc
     9001 ggatgcgctc acgcgcgcca aggtgcgtga gctggccctg agcgtcgcct gcgacattca
     9061 tccgctcaac aatttgcggg tgctgcgcta tctgcacgct ccgctgcaga tcgaagagga
     9121 acgtcgcaac gagtgggcgc ggcactggat ctcgctgggc ttcacggcga tcgaacacac
     9181 gctggagcgc agcggcgcgc tgagcactgg ctattgtttt ggcacgcagc caacgctcgc
     9241 cgactgctgt ctgattccac aggtgttcaa cgccaggcgc gtctcgctgc cgatggatga
     9301 gtatccctcg atccagcagg tgcatgatca ctgtatgcag ctaccggcct tcatcaaggc
     9361 atccccggga gctcagagcg atgccgaata ggcagcgcag gctgcgactg caagactatc
     9421 ttccctaccg tctgtcggtc gcaaccaatg ccgtctctcg gctgatcgcg cgcgcctacg
     9481 aatcccggtt cgcgctgaag attccgcaat ggcgtctgat cgcggtattg gccgatgagg
     9541 ggccgttgac gcaacaggct ctgtgcgtgc ggacgatcat ggacaaggtc acggtcatgc
     9601 gggcggcaca ggggttattg gagcgagggc tgatccgcag gcttcccaac gcgcaggacc
     9661 gtcgctcgca ccgtctgatt atgacagccg ccggtcagcg cctttacgat cagatcgtgc
     9721 cgatggcact ggaataccag gctcggctgc tccacggcat ggatcgcgcc gatatcctgc
     9781 agctcgagca gctgctgcgg cggctcgagc aatcggcgat cgcgctgagt gggggttaga
     9841 ggtaacaacc ctgaaggccg ttgcggcgat gcggtatcat taatgtcctt taatcttccg
     9901 gccacctcgc cttaaccggt acaagggtag cgtgcaccgg tgacagcagc cctttccgct
     9961 cggatcggac gcgcctgtgt cgttgccatc gcgtttctga cgctcgatgg ttgcagcgtc
    10021 ggtccgatct atcgtcatcc ggaaatgcca ctcccctcgg cgtggcgcaa tgctgcgccc
    10081 gtgccgcagg actggccgcg cgtggactgg tggcaggact tcggctccgc gcagctcaac
    10141 gaattcatga cgcaggccag ggccggcaac gacgatatcg cggcggcgat cgcgcgcgtc
    10201 aggcaggcgg acgcgcaggt gcgcatcgcc ggatcgccac tgctgcccac gatcgatctg
    10261 ggggcctccg ctgcgcgtca gcgcacgctg tcagtcagcg gcgggcgcac ccccatatat
    10321 aatgagttct cgccgcagct cagtgcgagc tacgagctgg atttctgggg caagaatcgc
    10381 gccgtgcgca gtgccgccgt cgccgccgcg caggccagcc gttacgatcg cgacaccatc
    10441 gcgctgagcg tctacaccag tgttgcgctg acctattttc aggcgctgga agcgcacgac
    10501 cggctggtgg tcgcacagga gaatctcgcc aacgcgcagc acatcctcga gggcctgcag
    10561 ctcgagcagc aggtgggtat cgccaccgca ctggatgtcg cacagcaggc cacgacggtt
    10621 gcgaccgtga acgccaccct gccgccgctt gcgctgcaac tgcagcagaa catcgacgcg
    10681 ctcgccattc tggcggggca gtcgccggag agcgtcaaac tgacgatcga gtccggactg
    10741 gacgatctga caccgccgcc ggttatcgcc ggcatgccct ccgagctgct gacgcggcgg
    10801 ccggacgtgg cgcaggcgga ggcgcaactg cgtgcagcca acgcggacat cgtcgccgcg
    10861 cgcgcggctt tcttcccgaa catcgagctg accgccagcg gaggatttgc cagcaccgtt
    10921 ctggccggtc tgctgcggcc ggggaacgag gtattctccg tcgccgccag tgccacgcag
    10981 accatctttg atttcggcgc gcgcagcggt cagctggcgt tttcgcgtgc ccgctatact
    11041 gagttgctgt cgagctatca caagaccgta ctcacggcgt tcggcaacgt cgaggatgca
    11101 ctggtggcgg ttcagcagac cgcagacgaa caggtgcggc agcaggatgc ggtcgataag
    11161 gccggtcgcg cattcgagtt cgcgcaactt cagatgcagg ccggcaccac taacgtgctg
    11221 accgagctga atacggagat tgctttgttt acggcgaaag atgcgctggt acaggtcaag
    11281 ttcctgcatc tgcaggcgct ggtcgatctg tatcaggctc tcggcggcgg ctggcatcag
    11341 gcgcaggagc cgacaacgtg aagattcgcg ggtggctgct agtcggcgcg gtgctgctgg
    11401 cggcgggttg gctctggctg acccgcaaga gcgccgatgc ggccggtcca aagcgagccg
    11461 cggccgccgt tgcggtggac agcgcgctcg ctgtgcgcca cgacgtgccg gtctatctgg
    11521 agggtctcgg caacgtgcag gccttctata ccgccaagat cagctcgcgc gtggacgggc
    11581 agctcgaccg cgtcggattc gtcgaggggc agctcgtcaa aaagggcgat ctgctcgctc
    11641 agatcgatcc ccggccgttg caggccgcac tggggcaggc cgacgccacg caggccaagg
    11701 acgcggcgca gctcgagagc gcccggcgtg atctggaacg ctatgccgtg ctcgcgccga
    11761 agaatctcgt cagccagcaa attctcgatg cgcagaaagc gctggtggcc cagctcgagg
    11821 cgcaggtcaa ggtcgatggc gcagccatcg acaacgcccg cactcagcta cagtacgcga
    11881 gcatcaccgc tccgttctcc ggacgtaccg gcatccgcct ggtggacccc ggcaatatcg
    11941 ttcacgccac cgataccacc ggaatcgtgg tgctgacgca gatccagccg atctcagtgg
    12001 tgttcacgct gccggaggat gccatctcgc aggtgacaca ggcgatttcc gccggaaagg
    12061 tgacggtcac ggcaatgtcg cgtgacgatc gtacgcagct cgactccggc acgctggcgc
    12121 tggtggacaa ccagatcgat atgacgaccg ggaccgtgaa gctgaaggcg acgttcgaca
    12181 atacgcgcaa tgcgctgtgg ccaggacagt tcgtcaacgt tcgcatcctg ctgcagcaac
    12241 gtcacgacgc gatcaccgtg ccgacgccgg ccgtccagcg cggcccggac ggaatgtttg
    12301 cttacgtcgt caaggccgat ttgaccgtgg agccgcgtcc gatcgaaacg gcggaggaag
    12361 ccgacggcct gacagtcatc accggcggcc ttgcggccgg cgagcgcgtc gtcaccacca
    12421 atcaatatcg actgcagcca ggggccagcg tgcagctgct ggcgccgacc tcgacccagt
    12481 ccgtggaaac cagcgccccc acctcatgag catttccgag ccgttcgttc gtcggccgat
    12541 cgcgacgtcg ctgctgatgt ccggcattct gctggtcggc atcgtcgcgt ggccactgct
    12601 gccagtggcg ccgctgccgc aggtggactt tccgacggtg gtggtgaccg gtcagctgcc
    12661 cggtgcaagt ccggagacca tggcctcatc ggtcgccaca ccgctcgaat atcagttcgc
    12721 gcagattccg ggcctgacgc agatgacctc gaccagcgtg ctcggcagct cgcaggtcac
    12781 cctgcagttc gacctggacc gcccgatcga tgcggccacc gaagatgtgc agctggcgat
    12841 ccaggccgcc ggcggccagc tgcccaagaa tctccccaac ccacccacca tccgcaagat
    12901 caatccggcg gactcaccca tcgtgattct gtcggtgcat tcggatgtgc tgccgctgac
    12961 gagcgttgat gattactgcg agaacgtcat tgcgcagcag atctcgcaga ttccaggtgt
    13021 cgcgcaggtc accatcggcg gacagcagaa acctgcggtg cgggtgcagg tcgacccggc
    13081 gaagatcgcg acgctgggac tgcagctgga ggacatcgcc ggcgtgattg ccgccgcaac
    13141 ggtcgacacg ccgaaaggat ccatcaatgg cccgctgcac agcttcgcga tctatgacaa
    13201 cgaccagttg ctgaaggcgg caccctggaa cgatgtcgtc gtcgcctaca agaatggcgc
    13261 accgatccgc attcgcgaca ttggcgtcgc ggtggacgga cccgagaaca atcagatcgt
    13321 gggctggcag aacaatcact acggcatcca gctgcagatc ttcaaacagc ccggtgccaa
    13381 cgtcg
//
LOCUS       DATMRM010000369        11386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_539195, whole genome shotgun sequence.
ACCESSION   DATMRM010000369 DATMRM010000000
VERSION     DATMRM010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11386
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539195"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..588
                     /locus_tag="VNZ06_11195"
     CDS             262..588
                     /locus_tag="VNZ06_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21363.1"
                     /translation="MQQPSYLDTLLYSLVMDLRVLPADAYPVRESEKLPPPLKRIVEM
                     TTQVGQSWGCWTDERHHWLFIAKLPLTRGKPLLHLNRYNEAGDLEETSNWYCDDKDQW
                     QRVTVP"
     gene            complement(844..1398)
                     /locus_tag="VNZ06_11200"
     CDS             complement(844..1398)
                     /locus_tag="VNZ06_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWW21364.1"
                     /translation="MLIAFNKPFRVACKFSPEPERKTLADYIDIRHVYPAGRLDTDSE
                     GLLLLTDDGILQARIAEPKHEIAKVYWAQVEGAPSQAAIGHLRQGVNLGDFMTQPAHV
                     NLIAEPQELWPRDPPIRHRAKIPTAWIELAIHEGKNRQVRRMTAKVGFPTLRLLRVAI
                     GKVSVKGLAPGTWREIAPDAPWSG"
     gene            complement(1409..3343)
                     /locus_tag="VNZ06_11205"
     CDS             complement(1409..3343)
                     /locus_tag="VNZ06_11205"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HWW21365.1"
                     /translation="MRILSIIPPMTQLNTPYPSTSYLTGFLRSRGFHAEQTDLSIALA
                     LKLLSAEGLRAIHQQVSALPRKQHTVATRFFAEHFEAYRTTISSVIRFLQGRDPSLAH
                     RIASRRYLPEGPRFRSLEHYVDGEQSEGDALDWAFGALGLQDRAKHIATLYLNEIANV
                     IRDAVDPRFEFVRYAESLAASQPTFDHLQTALAAPHSLVDAFLHALTLDVLSQHQPTL
                     VLITAPFPGNVYGAFRIAQTIKQTSPSIVTVLGGGYCNTELRAMNEARVFDYFDFITL
                     DAGERPLLALIEHLQNKRPLDRLVRTYTRERQYVDSKEPDIAFSECGTPTYAGLPLDD
                     YLSLLDLLNPMHRLWSDGRWNKLTVAHGCYWKKCSFCDVNLDYISRYDLAGTDVLIDR
                     IESLIRETGQTGFHFVDEAAPPKALKSLAKALLQRKVDISWWGNIRFEKPFDAALCQL
                     LADSGCIAVTGGLEVASDRLLKLMKKGISVEQVARVTRAFSDAGILVHAYLMYGFPTQ
                     TAQDTVDALEYVRQLFAAGCIQSGFFHRFTCTVHSPVGKHPEQYGVTLSAPRDTPFAN
                     NDIHFTDSTGVDHDLFGAALKKALYNYMHGIGLDLDVREWFEPRASVRRGKSKAARRR
                     ASHALPRTTVSPNLVERALA"
     gene            complement(3603..3932)
                     /locus_tag="VNZ06_11210"
     CDS             complement(3603..3932)
                     /locus_tag="VNZ06_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HWW21366.1"
                     /translation="MRISDIPFGTTDWSQVERTEHRGERGVAYWRTRQFGDIRVRMVE
                     YTAGYLADHWCEKGHILLCTAGELHTELKDGRRLILKPGMSYQVADGAEAHKSFAPLG
                     ATLFIVD"
     gene            4082..5308
                     /locus_tag="VNZ06_11215"
     CDS             4082..5308
                     /locus_tag="VNZ06_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21367.1"
                     /translation="MNAPQRASWRLVLLGNLVVFVSACSSGGSSAPPVIPSPAISRQP
                     GNQSVPMGLTATFSVVASGSALQYQWLKDSVEITGATANSYVTPSTSSPDNGSSYSVV
                     VTNAGGAVRSSAAMLTVTARAPAPGDLRFQQVDAPGTVNGWSVVSAVNTNVDGRGAVF
                     YSPAIGTPLYVGAGDCVAPAANDGTGCSWLYSVFAVSTPGVTTAYGGDLYDNFASDLQ
                     TGATGLLTFADSGIAPTSPNSVIHSLDIEQASDLFGLSWFQSSQQTGFVLAQNTVAPT
                     DLQAAAVGEGAAGRVITAISANNARVTYFAYAWQADTASVYEAQIVTASSADAPNAAA
                     QLASEGYIITATGLADGTGDILLVGTRVLGDTMPRPFMSASGAQVRTLQQQGYAIVGV
                     IFNAALNDPYTFLGER"
     gene            complement(5315..6100)
                     /gene="panB"
                     /locus_tag="VNZ06_11220"
     CDS             complement(5315..6100)
                     /gene="panB"
                     /locus_tag="VNZ06_11220"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWW21368.1"
                     /translation="MKLTAQSLRQMKAVAQIPMLTAYTAPMARSLEHAGIPVIMVGDT
                     VGMVEMGFDSTRHTTIEHMQYHIGAVRRGAPATHVIGDLPFASYSNPEIALSSARALI
                     NAGADSVKLEGPKFEVISHLVSNGIYVVGHTGLTPQTSKDFRQVGRSSEDAQRILDEA
                     VGIDKAGAFMLVLEHIPAALAEKITRAVTSPTIGIGAGAGCDGQVLVLNDAIGLGDYW
                     PPFSRQYAYVGKTIVDVAASFMKEVRDGTFVNSVVRINALRPD"
     gene            6420..7946
                     /locus_tag="VNZ06_11225"
     CDS             6420..7946
                     /locus_tag="VNZ06_11225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWW21369.1"
                     /translation="MSNALRAAIAAALALSACAVGPNFKQPAKPDASAYTREPLAPQT
                     QATDVSGGESQHFQLGAELPAQWWRLFESAQLDALIEQGIRNYPTIRAQQAALRQARQ
                     GVRAQQGVFFPQVSGSLNPQRSKSSASEAQTQDIVTLQAEGIPVPPGFTFPEDSPALY
                     TSVLTASVSVSYTFDLFGGERRSLENAQAQADEARFQLEASYLTLTSNIASTAIQMAS
                     VREQIAATHEIIALEEKELNLIQRQYDVGSRARADVLQQQSNLASFRATLPALQQQLS
                     VADHQLAVLVGHFPHDAEPVDFELSNLKLPQDLPVSLPSALVEQRPDIKAQEMVMRQA
                     NALVGVATANMLPQLTLQASSGDQAALFRQVISSSASIWSIAGNLAQPIFEGGTLRAK
                     RRAAIDAYDQAAAQYQQTVLNAFENVADSLTALENDALSVNAQFDAQSAAKASLDLIQ
                     RQYDAGAVGYVSLLAAQQSYQQARIAYVQAVAHRYADTVALFQSLGGGWWNRSKPQSL
                     "
     gene            8086..9225
                     /locus_tag="VNZ06_11230"
     CDS             8086..9225
                     /locus_tag="VNZ06_11230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWW21370.1"
                     /translation="MDQLVRPEFIVSPPRRRHFQMAIGAAILGILAITSWWLLRPHAP
                     AAAKSAAPLPLVTVVSPTLGEVSASVSLTGLISARNDMPIGTEGDVGRIAAVLVEAGD
                     RVHRGQVLARLNPITGQSGVDSAEASLAEATANAAVAQAEWRRAEQGPDLFSKEEDDR
                     RRTTALASDAKVKAAEAQLADMRNRLAHTTVIAPTDGIVLTRTAEVGQIAVPGTSVLF
                     RLARDGQVEMRGQVAESDLPKLKIGQRAQVRLDGVAKSFDGQIWQLGAVIDSNTRQGT
                     VRIALPSDNLDLRPGAFARADVHVDSVRGVLLPQTAVLADDKGNYVLVVGQDNKVQRR
                     DVTVAGARSEGLLVSAGLAGSERVIAIAGAFLRAGESVLIARPST"
     gene            9249..>11386
                     /locus_tag="VNZ06_11235"
     CDS             9249..>11386
                     /locus_tag="VNZ06_11235"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW21371.1"
                     /translation="MRRISAWAITHPIFPLVLFAVLLVFGIVSFIRLPITLSPDLSSP
                     FVSVYVNQPGAAPSEMETQIIQKVEGAVANVGNVKNITSFAAEGSATINIEFQIGTPI
                     DRATTDVRDAVARVRPDLPQGIQEPQVQRQDIDGGPIAYYAVSTTGMSEEQLSWFVDD
                     TISKRLLAVQGVAQVSRSGGVTREIRVDLDPARMESFGITAGEVNQQLRQVNLDAAGG
                     RATVGGSEQSIRVLGGARDALELGKVRLTMPDGRVARLNEIANVYDGTQEVRSICRLN
                     GRPATTFFVFKAKGFSDVETLKKIESELHKVTQETPAVSLKQVFTTVDDTKHSYNDSI
                     EALVGGALLAVVIVFVFLRDWRATIIAATAIPLAAMPTFAVMKWLGFTLNGISLLALS
                     LITGVLVDDAIVEVENITRHMKMGKSAYQAAFDAADEIGLAVVACSMTIIAVFLPVGM
                     MGGLIGQYFVQFGFTVAVAVFMSLLVARLVSPLLAAYLLKPHARLRGADGPVMQWYLR
                     VLRWSTTHRVKTTLAGLLVFLLSMAGLALIPKAFVPDSNGTLSALTIELPPGVLLSQT
                     ADASGQAYDILKRHPQVLNVVEALGEDQNGEVRIGTLYVQLAPPQQRKLTQMQLQKQL
                     SEELSVIPDARVHFQAQSDGDGRDLTLYVVGSNPQLAQNAAHELVTQMRTLKQLRDPR
                     INGDMARPELVVKPRLDEAAQLGVTVASIS"
BASE COUNT         2108 a         3417 c         3683 g         2178 t
ORIGIN      
        1 catcccgctg ggattcatat tggctgcgct ttgggcgtcg cggatctatt caaagcgccg
       61 caaacgctga tggaccgctt cctatcaatg ccaatttgac ttccgaggag cggtcatccc
      121 tgagtgtcaa ctacgggtca agtgcgggct agccagacga ttaaaccacc actattgcga
      181 attgctgccg cattgagcct tagacgcgag caccgtcggg cgttatcatc gcaaggtatg
      241 agaacgctca cgctacgaaa catgcagcag ccgtcatatc tcgacacgct gctgtattcg
      301 ttggttatgg atctgcgggt gctccccgca gatgcgtatc ccgtgcgaga gagtgagaaa
      361 ttaccgcccc ctctaaaacg gatcgtagaa atgacgaccc aggttgggca gagctggggg
      421 tgctggaccg atgagcgcca tcattggcta ttcattgcta agctgccgct gacgcgcggg
      481 aaacccctgc tgcatctcaa tcgctacaac gaggcgggtg acctagagga aaccagtaat
      541 tggtactgtg acgataaaga tcaatggcag cgcgttaccg tcccgtaagc ttggcgctac
      601 acatgcaccc cgcgacggcg cggatctggc ttgaaagaac ccggtcaaat tagctgctta
      661 gaggtccgcg cctcatagag ccggccgact ctcggcacac tgcttcaaca ggtggacgaa
      721 gggggcgtca tcgttgtcac cgacaaaggg agggacgacg tgcgctgggc tgaggtcacg
      781 cacgttcgcg tcatcacgac gagcgctggc ccctgggggg gcaggttgcc tgtcatctag
      841 cgtttagcca ctccacgggg catccggcgc aatctcgcgc caagtgcccg gtgccaatcc
      901 ctttacactg accttgccga tggccacacg taacaggcgc agcgtcggaa atcccacctt
      961 cgccgtcatg cgccgcacct ggcggttctt cccctcatgg attgcgagct caatccaggc
     1021 cgtcggaatt ttcgcgcgat ggcggatcgg cggatcgcgc ggccagagct cttgcggctc
     1081 cgcgatgaga ttcacgtgcg cgggctgtgt catgaagtcg ccgagattca ccccttggcg
     1141 caagtgcccg atggctgcct gagatggagc gccttccacc tgcgcccagt acaccttcgc
     1201 gatctcgtgc ttgggctcgg cgatgcgcgc ctgcaggatg ccatcgtcgg tgagcagcag
     1261 caggccttcg ctgtcggtat cgagacggcc agcgggatag acgtgtctga tgtcgatgta
     1321 atccgcgagc gtcttgcgtt ccggctccgg gctgaatttg caggcgacac ggaatggctt
     1381 attgaaggcg atgagcacgc gcgtgcgctc aagccagcgc gcgctcgacc agattcggcg
     1441 acaccgtcgt gcgcggcagt gcgtggctcg cgcggcggcg agctgccttg ctcttgccgc
     1501 gccgaacgga cgccctcggt tcgaaccatt cgcgcacgtc gagatcaagt ccgatgccgt
     1561 gcatgtagtt gtaaagtgct ttcttgagcg ccgcaccaaa caggtcatga tcgacgccgg
     1621 tggaatcggt gaagtggatg tcgttgttgg cgaacggggt atcgcgcggc gcagataacg
     1681 ttacgccgta ctgttccgga tgtttgccga ccggtgaatg cacggtgcag gtgaagcgat
     1741 ggaagaaacc cgactgtata caacccgccg cgaacaactg gcgaacgtat tccagggcgt
     1801 caacggtatc ttgcgcggtc tgagtgggga agccgtacat gagataagcg tgtacgagga
     1861 tcccagcgtc cgagaaggcg cgcgtgaccc gggccacctg ctcgaccgat atgcccttct
     1921 tcatgagctt gagcaggcga tcggatgcca cttccaatcc gccggtgacg gcgatgcaac
     1981 cgctatcggc cagtagctgg caaagcgcgg catcgaacgg cttctcgaaa cggatgttgc
     2041 cccaccatga gatatcgacc ttccgctgca gcagtgcctt cgcaagcgac ttgagcgcct
     2101 tgggcggcgc ggcttcgtcg acgaagtgga aaccggtctg tccggtctcg cgaatcaggg
     2161 attcgattcg atcgatcagc acatcggtgc ccgcgaggtc gtagcgcgag atgtagtcaa
     2221 ggttcacatc gcagaagctg catttcttcc agtagcaacc gtgtgcgacc gtcagcttgt
     2281 tccagcggcc gtccgaccag agtcggtgca tgggattgag caggtcgagc agtgacagat
     2341 agtcgtccag cggcaggccc gcataggtgg gcgtgccgca ttcagagaaa gcgatgtcgg
     2401 gctctttgga gtcgacatac tgacgctcgc gggtgtaggt gcgcaccagc cgatcgagcg
     2461 gtcgcttgtt ctggagatgt tcgatgagag cgagcagagg acgctcgccg gcatcgagtg
     2521 tgatgaaatc gaagtagtcg aacacgcgcg cttcgttcat cgctcgcagt tccgtattgc
     2581 agtacccgcc gcctaggacc gtcacgatgg aagggctggt ttgcttgatg gtttgcgcga
     2641 ttcgaaaagc accgtagacg ttgcccggaa agggcgcggt gatgagtacc agggtcggtt
     2701 gatgctgcga cagcacgtcg agggtcagcg catgcaggaa tgcatcgacc aggctgtgag
     2761 gcgcagcgag cgcggtttgc agatgatcga acgttggttg gcttgccgcc agcgattcgg
     2821 catagcgcac gaattcgaag cgcggatcga ccgcatcgcg aatgacgttc gcgatctcat
     2881 tgagatagag ggtagcaatg tgcttggcac gatcctgcag ccccagcgcg ccaaaggccc
     2941 agtcgagcgc gtcaccttcg ctttgctcgc cgtcaacgta gtgctcgagc gaccgaaagc
     3001 gtgggccttc gggtaggtag cgcctcgaag caatgcgatg cgccaggctc ggatcgcgac
     3061 cctgcagaaa ccgaatgacc gaggagatcg tcgtgcgata ggcctcgaag tgttcagcga
     3121 aaaaacgcgt cgccacggtg tgctgctttc gcggcagcgc gctcacctgc tggtgaatcg
     3181 cccgcaaacc ctccgccgac agcagtttga gggccagggc aatggacaga tccgtctgtt
     3241 ccgcgtggaa gccgcgcgat cgcagaaatc cggtgagata cgacgtggag ggatacggcg
     3301 tattgagctg cgtcattggc ggaatgatcg acaggatacg catttcggtc acattccgga
     3361 gtatatcgcg gtaggctgtc gtgctcttat ggagccgtcc tggaactttc cgcttacttg
     3421 aatcggatac gttttgacgg cagcgtccgg ctaacgacga caagatggta tgtcggcgac
     3481 gaggcggttg gtgctatgaa atgaatggtg tgatggggtg ggcgagctgt ctctcgctgg
     3541 agcacccgat gactaagctg gggcgatgaa tgggccccac gcggcgccgc tgcggtattc
     3601 gctcaatcca cgatgaacag ggtcgcgccc aacggagcga aggatttgtg cgcttcggcg
     3661 ccgtcggcaa cctggtagct catacccggc ttgaggatca ggcgtcgccc gtccttcagt
     3721 tcggtatgca gctcgccggc ggtacacagc agaatgtgcc ccttctcgca ccaatgatca
     3781 gcgagatatc cggcggtgta ttcgaccatg cgtactcgaa tatcgccgaa ctggcgggtc
     3841 cgccagtacg cgacgccgcg ctcgccccgg tgctccgttc gttccacctg cgaccagtca
     3901 gtcgtaccga aagggatgtc tgagattcgc acgtgctcaa tgctagcact gccttcccgg
     3961 tattttcgat cgcgaggagt ctgccgccgc gggcgtactg atgctattgt tacgcatggc
     4021 gcaggcgtct cgacgataag gggtagtcgc tgcagcagtc tcaaccaggc aaggtgatgg
     4081 cttgaatgcc ccacagcgcg cttcgtggcg cttggttctg ctgggtaacc ttgtcgtctt
     4141 cgtcagcgcg tgtagttctg gtggcagctc cgcgccgccg gtcatcccct ctccggccat
     4201 ctcgaggcag cccggcaacc agtccgtgcc gatgggtttg acggcgacct tctctgtcgt
     4261 cgccagcggt agcgctctgc agtaccagtg gttgaaggac agtgtcgaga tcaccggtgc
     4321 tacggccaac agctatgtca ctccgtcgac gtcctcaccg gacaatggat cgagctactc
     4381 ggtcgtggtg acgaatgcgg gaggcgcggt gcgcagcagc gcggccatgc tgacggtgac
     4441 cgcgcgcgcg ccggcgcccg gagatctgcg ctttcagcag gtggatgctc ccggaaccgt
     4501 caatggctgg agcgtcgtgt ccgccgtgaa taccaatgtg gacggccgag gcgcggtctt
     4561 ctactcgccc gccatcggca caccgctcta tgtcggcgcc ggcgattgcg ttgcgcctgc
     4621 ggccaacgac ggcacgggat gttcctggct ttacagcgtt tttgcggtgt ccacccccgg
     4681 cgtgacgacc gcatacgggg gcgacctgta cgacaacttc gcgtccgatc tgcagaccgg
     4741 cgccacgggc ctgttaacgt tcgctgatag tggcatcgca ccgacgtcgc caaactcggt
     4801 gatccattcc ctggatatcg agcaggccag cgatctgttc ggcctgtcct ggttccagtc
     4861 ctcacaacaa acgggctttg ttctggctca gaacacggtc gcgccaaccg acttgcaggc
     4921 tgcggccgtc ggggaagggg cggccggtcg ggtcatcacg gcgatctccg ccaacaatgc
     4981 tcgggtgacc tatttcgcct acgcctggca ggccgatacg gccagtgtgt acgaggcgca
     5041 gatcgtcacg gcatcgtccg ccgatgcacc caacgcggcg gcgcagcttg ccagtgaggg
     5101 ttacatcatc accgcaacgg gcctggctga cggcaccggg gatattctgc tcgtcgggac
     5161 gcgcgtgctg ggggatacca tgccgcgtcc cttcatgagc gcatcgggcg cgcaggtccg
     5221 gacgttgcag cagcagggct atgcgatcgt cggtgtgata ttcaatgccg cgctcaacga
     5281 tccgtatacc ttcctcggtg agcgctagcc tcgcctagtc cgggcgtagg gcgtttatcc
     5341 gcacgacgct gttgacgaat gtcccgtccc gcacttcttt catgaacgac gccgcgacgt
     5401 cgacgatcgt tttcccaacg taggcgtatt gccgggagaa cggcggccag tagtcgccca
     5461 ggccgatggc atcgttcagc accagcacct ggccgtcgca gccggcacct gcgccgatgc
     5521 cgatggtagg agatgtgacc gcacgggtga ttttttccgc cagtgcggcg ggtatgtgtt
     5581 ccagcaccag catgaaggcg ccggccttgt cgatgcccac cgcctcatcc agaatccgct
     5641 gagcgtcctc gctgcttcgg ccgacctgtc tgaagtcctt ggatgtctgc ggcgtgagcc
     5701 cggtgtgacc gacgacgtag atgccattgg agaccaggtg cgagatgacc tcgaacttcg
     5761 gtccttccag cttgacgctg tcagcgccgg cattgatcaa agcgcgcgca ctgctcagcg
     5821 cgatttccgg attgctgtag cttgcgaacg gcagatcgcc gatcacgtgt gtcgcgggcg
     5881 cgccgcggcg aacggcgccg atgtggtact gcatgtgctc gatcgtcgtg tggcgggtgc
     5941 tgtcgaagcc catctccacc atgccgacgg tatcgcccac catgatgacc gggataccgg
     6001 cgtgctcgag ggagcgggcc atcggtgcgg tgtacgccgt aagcatggga atctgcgcca
     6061 cggccttcat ttgccgcagc gactgagcgg tcagtttcat ttgtttacct gttgtgggga
     6121 catccccatt ttatcggata tcgcgccgcc aattgcccac tgccgcggcg cggccacgca
     6181 ggggctctga tttcgttcaa caaatagtgc aacgcgtacg cgccggcggg tcgagggcct
     6241 ggctgatgcc gtcaaaaagt actctccgcc tgaaacaatt ctgcgttgac ggggtattac
     6301 gcttgccagg ggcgaggacg cactgctaag cgcggccgca atctcaatgg agctggcagg
     6361 ttgtgcaccg aagttatcgt agaattggag agagaaatct ctcttgaggg aggctccccg
     6421 tgtccaatgc actccgcgca gcgatcgcgg cagcgctggc gttgtccgcc tgcgctgtcg
     6481 gacccaattt caagcagccg gccaagccgg atgccagtgc ttacacccgc gaaccgctgg
     6541 ccccgcagac gcaggccacc gatgtcagcg gtggagagtc gcagcatttt cagttgggcg
     6601 cggaactgcc ggcccagtgg tggcggttgt ttgagtccgc gcagctcgat gcgctgatcg
     6661 aacaaggcat ccgcaactat ccgaccatcc gggcacagca agcggcgctg cgtcaggcgc
     6721 gccagggtgt acgtgcgcag cagggcgtgt tttttcccca ggtcagcggg agcctgaacc
     6781 cgcagcgtag caagtcgagc gcctcggaag cccaaacaca agatattgtg acgttacagg
     6841 ccgagggcat accagtcccc cccggtttca catttccgga ggactctccg gcgctctaca
     6901 ccagcgttct gacggcgagc gtcagcgttt cctatacgtt cgatctgttc ggcggcgagc
     6961 ggcgctcgct cgagaatgct caggcgcagg cggatgaggc gcgcttccag ctggaggcaa
     7021 gctacctgac cctcacctcc aacatcgcct cgaccgcgat ccagatggcc tctgtgcgcg
     7081 aacagatcgc ggccacgcac gaaatcatcg cgctggagga gaaggaactg aacctgattc
     7141 agcgccagta cgatgtgggc agtcgcgcgc gtgccgacgt gctgcaacag cagtcgaatc
     7201 tggcgagttt ccgtgcaacc ctgccggcac ttcagcagca gttgtcggtt gcggatcatc
     7261 agctcgcagt gctggtcggg cactttccgc acgatgcgga gccggttgac ttcgagctgt
     7321 cgaatctgaa actgccgcag gacctgccag tgagtctgcc gtccgcgctg gtcgaacagc
     7381 ggcccgatat caaggcgcag gaaatggtga tgcgccaggc caacgcgctg gtcggagtgg
     7441 ccacggccaa catgctgccg caactgacat tgcaggcatc gagcggggat caggctgcgc
     7501 tgttcagaca ggtgatcagc tcatcggcgt cgatatggag cattgcgggc aatctggcgc
     7561 agcccatctt tgagggaggt acgctgcgcg ccaaacgtcg ggcagccatc gatgcgtacg
     7621 accaggcggc cgcgcagtac cagcagaccg tgctcaacgc ctttgagaac gtcgccgatt
     7681 cgctcactgc gctggagaac gatgcgctgt cagtgaacgc acagttcgat gcgcaaagtg
     7741 ctgcaaaggc gagtctggat ctgatacagc gtcaatatga cgctggcgcg gtgggttacg
     7801 tctcgctgct ggccgcgcag caaagctatc agcaagctcg tatcgcctat gtgcaggcgg
     7861 tggcgcaccg ctatgccgat acggtcgcac tgtttcagtc cctgggcgga ggctggtgga
     7921 atcgctccaa gccgcaatct ctgtagaaca atctctgtag aacatagggt gcaacttttg
     7981 tgcttgcaac gggttctata gttgaagaca aatgagaggg agttgaacgc cacgggcgtg
     8041 gatccctcgt aggctttgcg gtccaccaaa agcggaataa ctaagatgga tcaattggtt
     8101 agacctgagt tcattgtcag tccaccccgg cgacggcact tccagatggc catcggtgcc
     8161 gccatcctag gcatcctcgc aatcacttcg tggtggctgc tgcggcctca cgcgccggcc
     8221 gctgccaaga gcgccgcgcc cctgcctctg gtcactgtcg tgtcgccgac gttaggtgag
     8281 gtcagcgctt cggtatcgct caccgggctg atcagcgcgc gcaacgacat gcccatcggc
     8341 accgaggggg atgtcggccg cattgcggcg gtcctggtgg aagccggcga tcgcgtacac
     8401 cggggtcagg tgctggcgcg cctgaatccg atcaccgggc agtccggggt cgacagcgcg
     8461 gaggcatcgc tggccgaggc gaccgccaac gccgcggtgg cacaggccga atggaggcgt
     8521 gccgagcagg gtccggacct gttttcgaag gaagaggatg accggcgccg caccactgcg
     8581 ctggcctctg acgcgaaggt gaaagcagcc gaagcgcagc tggcggacat gcgcaaccgc
     8641 ctggcgcata cgacagtgat cgcacccacc gatggcattg tgctcactcg caccgcggag
     8701 gttggacaga ttgcggtgcc cggcacctcg gtgctgttca ggctggcgcg cgacggacag
     8761 gtcgagatgc gcggacaggt cgccgaatcg gatctgccga aactcaagat cggacagcgg
     8821 gcgcaagtgc gcctggacgg cgtggccaag tcgttcgacg gacagatctg gcagctcggg
     8881 gcggtcattg attcgaacac gcgccagggc acggtgcgca tcgcgctccc gagcgacaac
     8941 ctggatctgc gtccaggcgc gttcgcgcgt gccgacgtgc acgtggacag cgtgcgtggc
     9001 gtgctgctgc cacagacggc ggtgctggcc gacgacaagg gcaactacgt gctcgtggtc
     9061 ggtcaggaca acaaggtgca gcgacgcgac gtgaccgttg ccggggcgcg ctcggaaggt
     9121 ctgctggtca gcgcagggct ggcgggcagc gagcgcgtga tcgccatcgc cggagcattc
     9181 ctgcgcgccg gggaatccgt gttgatcgcc cgtccttcca cctgaagcct cctcaccgga
     9241 agcaacctat gagacgcatc tccgcctggg ccattacgca cccgatattt ccactggtgc
     9301 tgttcgcggt actgctggta ttcggcatcg tctcgttcat tcgactgccg atcacgctca
     9361 gtccggatct gtcctcgccg ttcgtttcgg tctacgtcaa ccagcccggt gcggcgccct
     9421 cggagatgga gacccagatc atccagaagg tcgagggcgc agtcgccaac gtcggcaatg
     9481 tcaagaacat cacgtcgttt gctgcggaag gctccgcgac catcaatatc gagtttcaga
     9541 tcggcacgcc gatcgatcgc gcgaccaccg atgtgcgcga tgccgtcgcg cgggttcggc
     9601 cggatctgcc gcagggcatt caggagccgc aggtgcagcg tcaggatatc gacggcggtc
     9661 cgatcgccta ctacgccgtc agcaccaccg gcatgagcga ggagcagctg tcgtggttcg
     9721 tcgatgacac gatcagcaag cgcctgctgg cagtgcaggg cgtcgcgcag gtgagccgct
     9781 cgggcggcgt cacgcgcgaa atccgcgtgg atctggaccc ggcgagaatg gagtcttttg
     9841 gcatcactgc aggcgaagtc aatcagcagc tgcgtcaggt caatctggac gcggccggcg
     9901 gacgggccac cgtgggcggc tcggaacaat cgatcagagt gctcgggggc gcacgtgacg
     9961 cgctggagct tggcaaagtc cggctgacga tgcccgacgg gcgcgtggcg cgcctgaatg
    10021 agatcgccaa cgtctacgac ggcacgcagg aagtgcgcag catctgccgg ctgaacggac
    10081 ggccggccac gacgtttttc gtcttcaaag ccaagggttt ttccgacgtc gagacgctga
    10141 agaaaatcga atccgaactt cataaggtca cccaggagac gcccgccgtc tcgctcaaac
    10201 aggtgttcac gaccgtcgat gacaccaagc attcctacaa cgactcgatt gaggcgctgg
    10261 tgggcggtgc gctgctggca gtcgtgatcg tgttcgtgtt cctgcgcgac tggcgcgcga
    10321 ccatcatcgc ggcaaccgcg atcccactgg cggcgatgcc tacgtttgcg gtcatgaagt
    10381 ggctcggttt cacgctcaat ggcatcagcc tgctggcgct gtcgctgatc accggggtgc
    10441 tggtggacga tgcgatcgtc gaagtggaga acatcacgcg ccacatgaaa atgggcaaga
    10501 gcgcctacca ggccgcgttc gatgcggccg atgagatcgg cctggccgtg gtggcgtgct
    10561 cgatgacgat catcgcggtg ttcctgccgg tcggcatgat gggcggactg atcgggcagt
    10621 attttgtgca gtttggtttc accgtcgcgg tagccgtatt catgtcgttg ctggtcgcgc
    10681 gcctggtcag tccgctgctg gcggcctacc tgctcaagcc gcatgcccgg ctgcgcggcg
    10741 ctgacggtcc ggtcatgcag tggtatctgc gggtcctgcg ctggtcgacg acacatcggg
    10801 tcaaaaccac gctcgcaggc ctgctggtgt tcctgctgtc gatggccggg ctggcgctga
    10861 ttccgaaggc gttcgttccg gacagcaacg gcactctctc ggcgctgacc atcgagctgc
    10921 ctcctggcgt gctgctgtcg cagacggcgg acgcctcggg gcaggcgtac gacattctga
    10981 agcgccaccc gcaggtgctg aacgtcgtcg aagctctggg tgaggaccag aacggggaag
    11041 tgcgaatcgg gacgctgtac gtgcagctgg cgccgccaca gcagcgcaaa ctgacccaga
    11101 tgcagcttca gaagcagctg tccgaggagc tgagcgtcat tcctgacgcc cgggtgcatt
    11161 tccaggccca atccgacggc gacgggcgcg atctgacgct ctatgtggtc ggcagcaatc
    11221 cgcagctcgc gcagaacgcc gcccacgagc tcgtgacgca gatgcgcacg ctcaagcagc
    11281 tgcgcgatcc gcgcatcaac ggcgatatgg cgcgtcccga gctggtggtg aaaccgcgtc
    11341 tggatgaggc cgcgcagctg ggcgtcacgg tcgccagcat cagcga
//
LOCUS       DATMRM010000370         3531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2992492, whole genome shotgun sequence.
ACCESSION   DATMRM010000370 DATMRM010000000
VERSION     DATMRM010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2992492"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2172
                     /locus_tag="VNZ06_11240"
     CDS             <1..2172
                     /locus_tag="VNZ06_11240"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF006515.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HWW21372.1"
                     /translation="AQGIEARWWDAREGLKAETHLLRSGRQNFLAATCDFAPDPVFRA
                     RCEEHTGVIVTQGFIASDAMNDTVLLGRGGSDTSAAYFAAKLTARRLEIWTDVPGMFS
                     ANPRHTPQARLLRSLHYDEAQEIASSGAKVLHPRCILPAKAHQIPISVFATQSPHLEG
                     THISADGGDGVAQVKAVCLKRGVTLVTLDTPGMWHEVGFLADAFAVFKQHGLSIDLVS
                     TSETNVTVSLDPAANSLDPEVMTRLTADLSALGRAQVIGPCTSVSLVGRNIRAILHQL
                     GEAFVLFEEQRIYLLSQAANDLNFTFVVDEGQGDRLVNELHERLIEPVRGGSILGPTW
                     QELFAPTVASRVPAPSWWRARRETLIGLAAQHECAFVYDLQVVHSAVDTLQSLKSVDR
                     VLYAMKANSNGAILQLLANRGVGFDCVSRGEIEHLLNCVPGLERSRILFTPNFAPRAE
                     YEWALAHNILVTIDNLFVLREWGELFRGHNVFLRIDTGTGRGHHHHVRTAGSHAKFGV
                     PMSEIHQLVELTRQHGITVSGLHAHSGSGIFDADNWSEVAGRLLDLGQRFPQLHTIDV
                     GGGLGVADGQDQRGLDIGELDTVLLAARQSRPGIALWIEPGRYLVAGAGALIARVTQT
                     KSKDDVHYVGIATGMNSLIRPALYGAWHEIVNLSRLDEPPTEIVNIVGPICESADFLG
                     HERLLPPTREGDVLLIANTGAYGRAMSSRYNLREPAVELLI"
     gene            complement(2233..2874)
                     /gene="hisIE"
                     /locus_tag="VNZ06_11245"
     CDS             complement(2233..2874)
                     /gene="hisIE"
                     /locus_tag="VNZ06_11245"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HWW21373.1"
                     /translation="MNFSDADIERIDWNKGDGLVPAVAQHLHTGTVLMLGYMNPEALK
                     LTLQRGRVVFYSRTRQRLWEKGEESGNILKLIDMRLDCDVDALLVTAIPTGPVCHTGT
                     ATCFGDQAVTDAERLSFLLELEQLIGKRIAEKPDGSYTARLYESGMRRMAQKVGEEGL
                     EVALAAVSEPDDPFLGECADLLYHLLVLLRARSLRLDTVIEEMKHRHADRSRR"
     gene            complement(2871..>3531)
                     /locus_tag="VNZ06_11250"
     CDS             complement(2871..>3531)
                     /locus_tag="VNZ06_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014998871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HWW21374.1"
                     /translation="TRYRDEGADELVFYDITASPEGRSVDRSWVNRVARVLDIPFCVA
                     GGIGSVSDAEAVLNAGAEKISVNSPALGDPGLIDRLSKRFGAQCVVVGIDSQSTPEGF
                     RVYQFTGDPSRSRDSRRDTLDWVREVQQRGAGEIVLNCMASDGTRHGYDVLQLKQVRE
                     ICTVPLVASGGAGAVEHFTAAFRDAAVDATLAASVFHSGAIAIPDLKRQLRGQGIEVR
                     L"
BASE COUNT          607 a         1123 c         1157 g          644 t
ORIGIN      
        1 gcgcagggca tcgaggcgcg ctggtgggat gcgcgtgagg gcctgaaggc cgagacgcat
       61 ctgttgcgct cgggccggca gaattttctg gcggcgacct gcgatttcgc ccctgatccg
      121 gtgtttcgcg cccgttgcga agaacatacc ggcgtcatcg tcacgcaggg cttcatcgcc
      181 agcgacgcga tgaatgacac tgtgctgctt gggcgcggcg gttcggatac ttccgccgcc
      241 tacttcgctg cgaaactcac cgcacgccgg ctcgagatct ggaccgacgt gccgggcatg
      301 ttcagcgcca atccccgcca cacgccgcag gcacgcctgc tgcgctcgct acattacgat
      361 gaagcacagg agatcgccag cagcggggcg aaggtgctgc acccgcgatg catcctgccg
      421 gcgaaggcgc atcagattcc gatctcggtg ttcgccacgc agagtcctca cctggaaggc
      481 acgcatatca gtgctgacgg cggcgatggg gtggcgcagg tcaaggcggt atgtctcaag
      541 agaggcgtca cgctggtcac gctcgacact ccgggcatgt ggcatgaagt cggctttctg
      601 gcggacgcat ttgcggtgtt caagcagcac gggctgtcga tcgacctggt gtccacctcg
      661 gagaccaacg tgacggtctc actggatccg gctgccaact cgctcgatcc ggaggtcatg
      721 acgcggctga ctgcggatct gtccgcactc gggcgcgcgc aggtcatcgg cccgtgcacc
      781 tccgtcagcc tggtcggtcg caacatccgc gcgatcctgc atcagctggg cgaggcgttc
      841 gtgctgttcg aggagcagcg tatctatctg ctcagccagg cggcgaacga cctgaatttc
      901 acgttcgtgg tggatgaggg ccagggcgat cggctggtga atgaactgca cgagcggctg
      961 atcgaaccgg tgcgcggcgg ttcgattctg gggcccacct ggcaggaact gtttgcccca
     1021 accgtggcgt cgcgggtgcc ggcgccgagc tggtggcgcg cgcgccgtga gaccctgatc
     1081 gggcttgccg cccagcacga gtgtgccttt gtctacgacc tgcaggtggt gcactcggcg
     1141 gtggacacac tgcagtcgct gaaatcggtg gatcgcgtgc tgtatgcgat gaaggcgaat
     1201 tcgaacggcg ccatcttgca gctgctggca aatcggggcg tcggtttcga ctgcgtctcg
     1261 cgtggggaaa tcgagcacct gctgaactgc gttccggggc tcgagcgcag ccgcatcctg
     1321 ttcacaccca atttcgcgcc gcgtgcggaa tacgagtggg cgttggcgca caacatcctg
     1381 gtgacgatcg acaatctgtt cgtgctgcgc gagtggggcg aactgttccg cgggcacaac
     1441 gtgttcctgc gcatcgatac cggcaccggg cgcggccacc atcaccacgt gcgcaccgcc
     1501 ggttcgcacg ccaaatttgg cgtgccgatg agcgagatcc atcaactggt cgagctcacc
     1561 cggcagcacg gcatcacggt gagcgggctg cacgcgcatt ccggcagtgg catcttcgat
     1621 gccgacaact ggtcggaagt ggccggccgc ctgctcgatc tgggccagcg ctttccacag
     1681 ctgcacacca tcgatgttgg cggtggactc ggcgtggccg acggacagga tcagcgcggc
     1741 ctggacatcg gtgagctcga tacggtgctg ctggccgcgc gccagagccg ccccgggatc
     1801 gcgctgtgga tcgagcccgg ccgctatctg gtggcaggcg ccggagcgct gatcgcgcgc
     1861 gtcacgcaga ccaagtccaa ggatgatgtg cactacgtcg gcatcgccac cggcatgaat
     1921 tcattgatcc gtccggcact gtatggcgcg tggcatgaga tcgtgaacct gagccgcctg
     1981 gatgagccgc cgaccgaaat cgtcaatatc gtcggcccga tctgcgagag cgccgatttt
     2041 ctcggacacg aacggctgct gccgccaacc cgtgaggggg acgtgctgct gatcgccaat
     2101 accggcgcct acggccgggc gatgagttcg cgctacaacc tgcgcgagcc ggccgtggaa
     2161 ctgctgatct gaacgggtga tcgaaagggc caggtcaggc atccggcgac gtggccgtaa
     2221 agcgtgcacc gcctagcgcc ggctacgatc cgcgtggcgg tgcttcatct cctcgatcac
     2281 ggtgtcgagc cgcagtgagc gcgcgcgcag cagcaccagc aggtgataca gcagatcggc
     2341 gcactcgccg agaaacgggt catccggctc gctgacggcg gccagcgcca cttccagtcc
     2401 ttcctcgccg actttttgcg ccatgcgtcg catccccgat tcatacagtc gggcggtgta
     2461 gctgccgtcg ggcttctcgg cgatgcgctt gccgatgagc tgctcgagtt cgagcagaaa
     2521 actcaggcgc tcggcatcgg tcacagcctg atcgccaaag caggtggcag tgccggtatg
     2581 acagaccggt ccggtcggaa tcgcggtgac gagcagggcg tcaacgtcgc agtcgagccg
     2641 catatcgatc agtttcagaa tgtttccgga ttcctcgcct ttctcccata gacgctggcg
     2701 ggtgcggcta tagaacacca cccgtccgcg ctgcagtgtc agcttcagcg cctccggatt
     2761 catgtagccc agcatcagca ccgtaccggt atgcaggtgc tgcgcgactg cgggcaccag
     2821 gccgtcaccc ttgttccagt cgatgcgttc gatgtcggcg tcactgaagt tcaaagtcgt
     2881 acctcgattc cttggccgcg cagttgacgt ttgagatccg ggatggcgat cgcgccgctg
     2941 tggaacacgc tggctgccag cgtcgcgtcg accgcagcat cgcgaaacgc agcggtgaag
     3001 tgctcgacag cgccggcgcc accggacgcg accagcggca ccgtgcagat ctcgcggacc
     3061 tgtttgagtt gtaggacatc gtacccatgt cgtgtaccgt cggaggccat gcagttgagc
     3121 acgatttccc cggcgccgcg ttgctgcact tcacgcaccc agtccagtgt atcgcgacga
     3181 ctgtcgcgcg agcgtgacgg atctccggtg aattggtaaa cccggaagcc ctccggggtc
     3241 gactggctgt cgatgccgac cacgacgcac tgggcgccaa agcgcttgct gagccgatcg
     3301 atcagccccg gatcgcccag cgccggtgag ttgaccgaga ttttctcagc gccggcattg
     3361 agtacggctt cggcgtcgct gaccgagccg atgccaccgg ccacacagaa cggtatatcg
     3421 agcacccggg cgacgcgatt cacccaggag cggtccaccg agcggccttc cggacttgcg
     3481 gtgatgtcgt agaacaccag ctcatccgcg ccctcgtcgc gatagcgcgt c
//
LOCUS       DATMRM010000371         4539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3601796, whole genome shotgun sequence.
ACCESSION   DATMRM010000371 DATMRM010000000
VERSION     DATMRM010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3601796"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VNZ06_11255"
     CDS             complement(<1..781)
                     /locus_tag="VNZ06_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWW21375.1"
                     /translation="MPLENFHPAVARWFASQFTAATAPQLSAWPAIAAGGHALIAAPT
                     GSGKTLAAFLAAIDALVRQGIAQELTDATQVVYVSPLKALSNDVQRNLEQPLAGISAE
                     LEAMGLCCPPIRAQVRTGDTSQIDRVAMRRTAPHILVTTPESLYLLLTSESGRRMLSS
                     TRTVIVDEIHAVAASKRGAHLALSLERLAGLCDRPVQRIGLSATQKPIERIARFLVGG
                     AAVDADVTIVDSGYVRRRDLSLVLPDAPLDAVMSNEVWENIY"
     gene            832..1362
                     /locus_tag="VNZ06_11260"
     CDS             832..1362
                     /locus_tag="VNZ06_11260"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWW21376.1"
                     /translation="MSAYTLFATAVGTCGIAWGTAGIVGVQLPECNPSATLSRLLRKL
                     GGDASPGLPPSDVQRTIDDMTALLAGEHRDLRHARLDLTAIPEFFQRVYAIVRQIPPG
                     QTLSYGEVATRCADPAAARTVGQAMARNPFPIIVPCHRVLAAHGKNGGFSAHGGVSTK
                     LRLLAIEGAGTLSLPW"
     gene            1419..1994
                     /locus_tag="VNZ06_11265"
     CDS             1419..1994
                     /locus_tag="VNZ06_11265"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWW21377.1"
                     /translation="MNAMKFAVSAAVLSVAVATTAWAAPTTYKSDPNHTFVRFSYQHM
                     GFSTQESRFNKVTGTITFDPAARSGSVDISIDTKSVDTGSDLFNGHIQGADFLDTTNF
                     GSATFRSTSVKFEGDRPVSIEGNLTLKGVTKPVTLMVTDFKSGQNMMKKDAIGADATA
                     KISRTAFNMSKYVPNVGDDVTLSIAIEAAAP"
     gene            complement(2050..2436)
                     /locus_tag="VNZ06_11270"
     CDS             complement(2050..2436)
                     /locus_tag="VNZ06_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21378.1"
                     /translation="MRTAVLIAVCIAALGGCLTPVKRVSTPLVLTPFDDDPVSFLVVR
                     RFTPGEVAIVRVCVAPDRTIISADLVESSGDARFDSMALAWAQSVRLRSAPASGTPMA
                     ACGEVRVEIRVPAEPRVFSGSDTSLG"
     gene            2503..2988
                     /locus_tag="VNZ06_11275"
     CDS             2503..2988
                     /locus_tag="VNZ06_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWW21379.1"
                     /translation="MRHAKSSWDNPDLADVDRPLSARGRHAARKMGRRLAKRHADPDL
                     IVSSPAVRALKTARILARRLNHPRRGILVNPRIYACTAEHLWHTVQVLEDRFLTVILV
                     GHNPEITALAHRFCGQITHMPTCAVVRLRFDIDCWRDIGTTMPLQTEFDYPKRPKRGR
                     T"
     gene            2985..>4539
                     /locus_tag="VNZ06_11280"
     CDS             2985..>4539
                     /locus_tag="VNZ06_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWW21380.1"
                     /translation="MNSRARLILRVVLLVIVVIVLGRRYWHLPGTEARPQLTASPAAA
                     AKEAQQLRVGSVVFKSCEIGHRAEAGPANATAAAFCTRFDVPEDWNAPQGRHIQLHVA
                     LVKSGAAHPPPDLVTYLDGGPGGAATETYPAIVQAFAPLKEKRDILLIDQRGTGASNP
                     LRCEQQKDEEKSAVKDLARGVELVQKCLEHLRTHSAPEHYTTTDANRDLEAVRQALGA
                     PPLNLIAISYGTRAAQQYAGHYPGAIRSVVLDSTVPNPLVLGSEHARNLEAALRAEFA
                     LCTQNPRCEKNFGDTYKTLYRLRDHLRGHPEDVAMRDPNTFEPTRLTMSAPDLAGIVR
                     MYLYSPLTASLLPLMLHEADHGNYEPLLSQKKLLSDSLGAEISGGMELSVICSEDADL
                     MSARPEDADTLIGNALIERIKAACSVWPKGQRPQDFHQPWTSALPVLVLAGQYDPVTP
                     PAYGETVVKTLSHSRLLIVPGQGHSVIGAGCMPKLVSQFIDDLQPGKIDADCLTELGD
                     TPAFVDFNGP"
BASE COUNT          891 a         1459 c         1403 g          786 t
ORIGIN      
        1 cgtagatgtt ctcccagacc tcattcgaca tcaccgcatc gagtggcgca tccggcaaca
       61 ccaacgacag atcgcggcgg cgcacgtagc cgctgtccac gatcgtgaca tcggcgtcga
      121 cggcagcacc gcctaccagg aagcgggcga tgcgctcgat cggtttttgt gtggccgaca
      181 gaccaatgcg ctgcaccggt cggtcacaca atcccgccag tcgttccagc gacagtgcca
      241 ggtgagcacc gcgtttactg gcggcaaccg catggatctc atccacgatg acggttcgcg
      301 tactcgacag catgcgacgc ccggactcac tggtgagcaa cagatacagc gactcgggtg
      361 tggtgaccag aatgtgcggc gcagttcggc gcatcgcgac gcgatcgatc tgcgaggtat
      421 cgccggtgcg cacctgggca cggatcggcg ggcagcacaa ccccatggct tcaagctcag
      481 cgctgatgcc agcgaggggc tgctcgagat tgcgctgcac gtcgttcgac agcgccttca
      541 gcggggagac atataccacc tgtgtcgcgt ccgtgagctc ctgcgcgatg ccctgacgca
      601 cgagcgcatc gatggccgcc aggaaagcgg ccaaggtctt acccgaaccg gtgggtgccg
      661 cgatcagtgc atgaccgccc gccgcgatgg ccggccaggc cgacagctgc ggggcggttg
      721 ccgctgtaaa ctggctggca aaccagcgcg cgacggccgg gtgaaagttc tccagcggca
      781 tggatcaatt ctactcccgc acccccggcg cgcggtaaaa tccgccccac catgagcgcc
      841 tacacgttgt ttgcgaccgc cgtgggtacc tgtggcatcg cctggggtac ggcaggcatc
      901 gttggggtgc aattgcccga atgcaatccg tcggcgacgt tgtcgcgcct gctgcgcaag
      961 ctgggcgggg atgccagtcc cgggctgccg cccagcgacg tgcagcggac catcgacgac
     1021 atgacggcgc tgctggccgg cgagcaccgt gatctgcggc acgcgcgtct ggatctgacc
     1081 gcgataccgg aattctttca gcgggtatac gccatcgtac gacagattcc gccgggccag
     1141 accctgtcct acggcgaagt ggccacgcgc tgcgctgatc cggcggccgc ccgaacggtc
     1201 ggccaggcca tggcgcgcaa tcctttcccg atcatcgttc cctgccatcg cgtgctggcc
     1261 gcgcacggca agaacggcgg cttttcggca cacggcgggg tcagcaccaa actcaggcta
     1321 cttgcgattg agggcgccgg caccctgtcc ctgccctggt agcacaggat aagatgcgca
     1381 tcccctagaa tctgcaaatt tccaccgaag gagtcccgat gaatgcaatg aagtttgccg
     1441 tctcggccgc ggtcctgtcg gtcgcggtcg cgaccacggc ttgggctgca ccgaccacct
     1501 acaagagcga tccgaaccac accttcgtgc gtttctcata ccagcatatg ggcttttcga
     1561 cgcaggagtc gcgcttcaac aaggtcaccg gcacgatcac tttcgacccc gccgccagga
     1621 gtggctcggt ggacatctcc atcgatacca agtccgtgga cacaggctcg gacctgttca
     1681 atgggcacat tcagggtgct gattttctgg acaccacgaa tttcggaagc gccaccttca
     1741 ggtcgacctc ggtgaagttc gagggtgacc gaccggtcag tatcgaaggc aatctgacgc
     1801 tgaagggtgt gacgaaaccc gtcacgctca tggtcaccga tttcaagagc ggacagaaca
     1861 tgatgaaaaa ggatgcgatc ggcgcggacg cgaccgccaa gatcagtcgc accgccttca
     1921 acatgagcaa gtacgtgccg aacgttggcg acgatgtcac gctctcgatc gcgatcgagg
     1981 ctgcggcccc ctgagccgtt cccttcatca ttgacagcgg gtgcgatcca agcgcgatcg
     2041 cacccgctgt catccgagtg aagtgtccga tcccgagaat actctgggct cggcaggcac
     2101 gcggatttcc acccgtacct ctccgcaggc agccatcggg gtcccgctgg caggagcgct
     2161 gcgcaacctg accgactgcg cccacgccag tgccatgcta tcgaagcgcg catcccccga
     2221 agactcgacc agatcggcac tgatgatggt tcggtcggga gcaacacaga cccggacgat
     2281 ggcgacttcc cccggagtga atcgccgtac caccaggaat gaaaccggat catcgtcgaa
     2341 cggtgtcagc acaagagggg tcgaaacgcg tttcacaggc gtgaggcagc cgcccagggc
     2401 ggcgatgcac acggcgatga ggacggcggt tcgcatcgat ctatcccgcc cagtgagtat
     2461 acgcctcggc gcgcggggtt tgatttgaag acgctttttc tcgtgcggca cgcaaaatcc
     2521 agttgggaca atcccgatct ggccgacgtg gatcgcccac tgagtgcccg cggcaggcat
     2581 gccgcccgca aaatggggcg ccgactggcc aaacgtcacg ccgatccaga tctgatcgtc
     2641 tcgagcccgg cggtgcgggc tctcaagacc gccagaatcc tcgcccgccg actcaaccat
     2701 ccccgccgag gcatcctggt caacccgcgt atctacgcct gcaccgcaga acacctgtgg
     2761 cacactgtgc aggtcctgga ggatcggttc ctgacggtca tactggtggg gcacaacccg
     2821 gaaatcactg ccctggcgca ccgcttctgc gggcaaatca cgcatatgcc cacctgtgcc
     2881 gtcgtgcgct tgcgtttcga catcgattgc tggcgagaca tcggcacgac gatgccgctg
     2941 cagaccgaat ttgactatcc aaagagaccc aagaggggac gcacgtgaat agcagggcgc
     3001 ggctgatcct acgcgtcgtt ttgctcgtga tcgttgtcat cgtgctcggg cggcgctatt
     3061 ggcatttgcc gggtaccgaa gcgcgcccgc agctgaccgc gagccccgca gccgcggcca
     3121 aagaggcgca gcagctacgg gtcggaagcg tcgtgttcaa atcctgtgag attggccatc
     3181 gcgccgaagc cggccctgcg aatgccaccg cggccgcgtt ttgtacgcgc tttgatgtgc
     3241 cggaagattg gaacgcgccg caagggcgtc acattcagct gcacgttgct ctggtaaaga
     3301 gtggcgccgc gcatccgccg cccgatctgg tcacttatct ggacggtgga cccggaggtg
     3361 cggcgaccga aacctatccg gccatcgtgc aggcgttcgc gcccctcaag gagaagcgtg
     3421 acatcctgct cattgatcag cgcggtaccg gcgcctccaa tccgctgcga tgcgagcagc
     3481 agaaggacga ggagaaaagc gcagtcaagg atctcgcccg gggtgtcgaa ctggtgcaga
     3541 aatgcctgga acacctgcgc actcactcgg caccggagca ttacacgacc acggatgcga
     3601 accgcgatct ggaagccgtt cgacaggcac tcggcgcacc gccgctgaac ctcatcgcta
     3661 tctcctatgg cacgcgcgcg gctcagcagt acgccggcca ctacccgggg gcgatccgca
     3721 gcgtggtgct ggacagtacg gtaccgaacc cgctggtgct tggcagcgag cacgcgcgca
     3781 atctggaggc agcgctgcgc gcggagttcg cgctgtgcac acagaacccg cgctgcgaga
     3841 agaatttcgg cgacacttac aagacgctct atcgattgcg cgatcatctg cgtggccatc
     3901 ccgaagatgt cgcgatgcgc gatccgaata cctttgaacc gacgcgtctg acgatgagcg
     3961 ccccggatct ggccggtatc gttcggatgt atctctacag tccactgacc gcctcgctgt
     4021 tgccgctgat gctgcacgag gccgatcacg gcaattacga gccgctgctg agccagaaga
     4081 agctgctatc ggactcgctg ggtgcggaga tcagcggcgg catggaactg tcggtcatct
     4141 gcagcgaaga cgccgatctg atgtcggctc gccccgagga tgctgacaca ctcattggca
     4201 acgcgttgat cgagcggatc aaagcagcct gcagtgtgtg gccgaaaggc cagcggccgc
     4261 aagacttcca ccagccctgg acctcggcgc taccggtact ggtgctggcg gggcaatacg
     4321 atccggtgac accgccggcc tatggtgaaa cggtggtgaa gaccctgagc cattccagac
     4381 tgttgatcgt accgggccag ggacactcgg tcatcggcgc cggctgcatg ccgaaactgg
     4441 tgagccagtt catcgacgat ctgcaaccgg gcaagatcga tgccgactgc ctgacggagc
     4501 tcggtgatac accagcattt gtcgatttca acggaccgc
//
LOCUS       DATMRM010000372         3906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1789726, whole genome shotgun sequence.
ACCESSION   DATMRM010000372 DATMRM010000000
VERSION     DATMRM010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789726"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2324
                     /locus_tag="VNZ06_11285"
     CDS             <1..2324
                     /locus_tag="VNZ06_11285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW21381.1"
                     /translation="ASGMGISLASGANALKTAAAVEEALHRFEPGFPAGMHFAVAYDT
                     TPFVRKSIVEVLKTLTEAIALVFLVMWLFLQNLRATLIPTIAVPVVLLGTFAVLKAAA
                     FSINMLTMFAVVLAIGLLVDDAIVVVENVERLMTDEGLSPRDATRKSMQQITGALIGI
                     ATVLSAVFVPMAFLNGSSGVIYRQFSLTIVAAMVLSVIMAIVVTPALCATLLKPVAKG
                     EHHSNRGLFGWFNRFFDHSSRRYQGLVALILGRPTRFVLVYAALASIMGLLFFRLPTA
                     YVPEEDQGYLFTVLQTPVGATLQRTLNAMEKIKDYFLTQERDAVDNTFLVGGFSPTGA
                     AQNVGFGYIVLKDWSLRRSKNLSAAAIQGRAYRALAGIGDAMAFVFAPPAIAELGNSA
                     GFDFYLKDNKGKGHAALIAVRNQLLGMAMKDPLLANVRPAGIEDAPQFRLDINPQKAT
                     SLGLSMEDVNDTLQAAWGGRYIDDFLDRGRVKRVIIQSDAPFRMNPEDINGWYVRNKA
                     GGMVPVASFASSHWDFGPPRLERFNGVSAVEINGQPAPGVSSGQAMKEIEKLVGELPP
                     GYSVDWTGESYQERAAGAQTPALYTLSIIVVFLCLAALYESWSIPTAILMAVPLGIVG
                     AVLTTTLRGLERDIYFQVGMLTTIGLTSKNAILIVEFAKDYVGSGMNVIEATLRAVRD
                     RLRPILMTSLAFGLGVLPLAAATGAGAGAQRAIGTGVFGGMLGGTFLGIFFIPLFFVV
                     VQRVFHGVHEHTPLEPAPESVPLIPPPATPGST"
     gene            2324..3796
                     /locus_tag="VNZ06_11290"
     CDS             2324..3796
                     /locus_tag="VNZ06_11290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWW21382.1"
                     /translation="MHACARTLGVVVFGLLSSCTLEPRYRVPPLPVPDQWPLPENGAP
                     LPAADANPTDAPAWEIGWREFFVDRQLQQLIAQALTNNRDLRVSILNVELARAQYHIQ
                     RADLSPQVNANGSYTREKLPPALIFGGTLPSGTKIAIEKIYQVGVGVTSWEIDLFGKV
                     QSQTHAALQQYLAQEQARRSAQLSLIAQVVSAYLTLAADRELDRLALETLKSQEASYE
                     LTKKRHDSGAVSGLDLAQAQTTVESARADAARFEGNVAQDLDLLTLLVGAQIDTAQLP
                     NSMDANPIGLAPLPPSLPSTVLLRRPDILQAEHVLRSANADIGAARAAFFPTISLTGD
                     VGSASTTLSGLFKAGSMTWLVEPQATLPIFHGGALVGNLSAARTQREIAVAQYEKAIQ
                     SGFRDVADALALTATLDRQHRAQQALVDATSQAYELSQQRYKTGADSYLTLLDAQRSF
                     YAAQQGLISTRLAEQSNRVTLYKALGGGWQEHSGKTASNQ"
BASE COUNT          703 a         1194 c         1242 g          767 t
ORIGIN      
        1 cggcttcggg catgggcatc tcgcttgcct ccggcgccaa cgcgctgaaa acggcagccg
       61 cggtcgaaga ggcactgcac cgcttcgagc ctggatttcc cgccggtatg cactttgccg
      121 tggcttacga cacgacgccg ttcgtgcgca agtcgatcgt cgaagtgctg aagacgctga
      181 ccgaagcaat cgcactggtg ttcctggtga tgtggttgtt cctgcagaac ctgcgcgcga
      241 cgctgattcc aaccatcgct gtgccggtgg tgctactggg taccttcgca gtactcaagg
      301 ccgcggcgtt ttccatcaac atgctcacaa tgttcgcggt ggtgctggcg atcggcttgc
      361 tggtggacga cgccattgtg gtggttgaga acgttgaacg cttgatgacg gacgaaggcc
      421 tgtcgccgcg ggacgccact cgcaaatcga tgcaacagat caccggtgcg ctcatcggca
      481 ttgccacggt gctgtctgcc gtcttcgtac cgatggcatt tctcaatggt tccagcggcg
      541 tgatctaccg ccagttctcg ctgacgatcg tcgcggccat ggtgctgtcg gtgatcatgg
      601 ctatcgtcgt gacgccagcg ctgtgcgcca cgttgctcaa accggtggcc aagggcgagc
      661 atcattccaa ccgcggtcta ttcggttggt tcaatcgatt ctttgatcac agcagccggc
      721 gctaccaggg cctggtggct ctcatactgg gccgtccgac gcgctttgtg ctggtgtacg
      781 ccgcgttagc gtcgatcatg gggctgctgt tctttcgcct gccgaccgct tacgtgcccg
      841 aggaggatca aggctatctc tttaccgtgc tgcagacgcc ggtcggcgcc acgctgcagc
      901 gcacgctcaa cgcgatggaa aagatcaagg attacttcct cacgcaggaa agggacgccg
      961 tcgataacac cttcctggtg ggaggtttca gccccaccgg agctgcacaa aacgtcggct
     1021 tcggctatat cgtgctgaag gactggagct tacgccggtc gaaaaatctg agcgccgcag
     1081 ccatccaggg tcgcgcctat cgcgcgctgg cgggaatcgg cgacgcgatg gcctttgtgt
     1141 ttgcgccgcc ggcgatcgct gagcttggga attccgccgg cttcgatttc tatttgaagg
     1201 acaacaaggg caaggggcat gctgcgctga ttgccgtccg taaccagtta ctaggcatgg
     1261 ccatgaagga tccgctgctc gcaaacgtgc ggccggcggg catcgaggac gcgccgcaat
     1321 ttcggctcga tatcaatccg cagaaagcga cgtcgctcgg tctgtcgatg gaggatgtca
     1381 acgacacgct gcaggctgcc tggggcggcc gttacatcga tgacttcctg gatcgcggac
     1441 gcgtcaagcg tgtgatcatt cagagcgacg ccccgtttcg catgaatccg gaagatatca
     1501 acggctggta cgtgcgcaac aaggcgggcg gcatggtgcc ggtcgcgtcc ttcgcgagct
     1561 cacactggga tttcggaccg ccgcgactgg agcgattcaa cggcgtgtcg gcggtggaga
     1621 tcaacggtca gccggcaccg ggagtgagct caggccaggc gatgaaggag atcgagaaac
     1681 tggtgggcga attgccgcca ggctattccg tggactggac cggggagtcc tatcaggagc
     1741 gtgcggctgg cgcacaaacc ccggccctgt acacactatc catcatcgtc gtatttctgt
     1801 gtctggccgc gctctatgag agctggtcga taccgaccgc gatcctgatg gcggtgccac
     1861 ttggcatcgt cggtgcggtg ctcaccacca ccctgcgcgg actggagcgc gacatctact
     1921 ttcaggtcgg catgctcacg accattggac tgaccagcaa gaatgcgatc ctgatcgtcg
     1981 agttcgccaa ggattacgtc gggtcgggta tgaatgtgat cgaggcgacg ctgcgcgcgg
     2041 tgcgcgatcg tctgcgaccg attctgatga cctcgctggc atttggcctg ggcgtattgc
     2101 cgctcgcggc cgcgaccggc gcgggcgccg gcgcgcaacg cgcgattggc accggggtgt
     2161 tcggcggcat gctgggcggc acgttcctgg ggatcttttt catcccgctg ttcttcgtcg
     2221 tcgtgcagcg cgtgttccat ggcgtgcatg aacacacgcc gctggaaccg gcacccgagt
     2281 ccgtgccgct gattccaccg ccagcgacgc cgggtagcac ctgatgcatg cctgcgcacg
     2341 cactctgggc gttgtggtgt tcggcctgct cagcagctgc acgctcgaac cgcgctatcg
     2401 cgttccgccg ctgccggtgc ctgaccagtg gccgctgccg gagaatggcg cgcccttgcc
     2461 ggccgcggac gcgaatccca cggatgcgcc cgcgtgggaa atcgggtggc gcgagttctt
     2521 cgtcgatcgg cagctgcagc agctcatcgc gcaagcgctg actaacaacc gcgacctgcg
     2581 cgtctcgata ctcaacgtcg agctggcgcg cgcgcagtac cacattcagc gagcagatct
     2641 gtcgccgcag gtcaatgcca acggctccta tacccgcgag aagctgccac cggcgctaat
     2701 cttcggcggt acgcttccca gcggtacgaa gattgcgatc gaaaagatct atcaggtcgg
     2761 cgtgggcgtg acgtcttggg aaattgatct gtttggtaag gtgcagagtc agacgcatgc
     2821 ggccttgcag cagtatctgg cgcaggagca ggcacgacgc agcgcgcagc tgagtctgat
     2881 cgcgcaagtg gtcagtgcct atctgacctt ggcagcggat cgggaactgg atcggctggc
     2941 gctcgaaacg ctcaagagtc aggaagcttc ctacgagctc accaagaagc gccacgattc
     3001 cggtgcagtc tcgggcctgg atctcgcaca ggcgcagacc acggtggaga gcgcacgcgc
     3061 cgatgccgcg cgctttgaag gcaacgtcgc gcaggacctg gatttgctca cgctcctggt
     3121 gggcgcacag atcgataccg cgcagctgcc gaacagcatg gatgccaatc cgatcggatt
     3181 ggcaccgttg ccgccgagtc tgccatccac ggtgctcctg cgccgaccgg acattctgca
     3241 ggccgagcac gtcttgcgtt ccgccaacgc cgatattggc gcggcccgtg cggcgttctt
     3301 tccaaccatc agtctcaccg gtgatgtcgg ctcggcgagc acgactctct cgggactctt
     3361 caaggccgga tcgatgacct ggttggtaga accccaggcg acgctgccga tctttcacgg
     3421 cggcgcgctc gttgggaatt tgtccgcggc tcgcacgcag cgagagatcg cggtggctca
     3481 atacgaaaag gcgattcaat ccggctttcg cgacgtggcc gatgcgctgg cattgaccgc
     3541 tacgctggat cggcagcacc gcgcgcagca agcactggtg gatgccacct cacaagcata
     3601 tgaactctca caacagcgct acaagaccgg cgccgacagc tatctcacgc tgctcgatgc
     3661 gcagcgcagc ttttatgccg ctcaacaggg gctgatttcc acccggcttg ccgagcaaag
     3721 caaccgggtg accttgtaca aggctctagg tggcggatgg caggaacaca gtggcaaaac
     3781 cgcttccaat cagtgagtcc ggcggcaacg gcgcggccga gcgtgccgcc caccaacgtc
     3841 aacgcattct ggatgcggcg gagagatgtt tcatcgatcg tggctttcac gccgcgggca
     3901 tggcgc
//
LOCUS       DATMRM010000373         2592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3925723, whole genome shotgun sequence.
ACCESSION   DATMRM010000373 DATMRM010000000
VERSION     DATMRM010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3925723"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VNZ06_11295"
     CDS             <1..976
                     /locus_tag="VNZ06_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWW21383.1"
                     /translation="LLTALPARIETVMVIDPDVKICGRHEGSAVELERVVADLQQSGA
                     AAACPRVAIDRDGFLARFQALEYALAFVVGRRSLADFGVTSGVSIYRRDALEHALDQH
                     SLSIYAEDLENAVILLHDGERIYYDGRLVVSTEGPGSLRRWFSQRVGWYYGLLRVYTQ
                     RFHELWRIGRRTPFAMYNFIAYMGVLGLGLHLLRIVSAALLLVSVAAILDNLFVFNLL
                     PNAGAINPVYFAAALGSYLALGALALFVVVPRSERAYIAPIVPIYFLYVLMHVAPITV
                     GFANWVALRLWGRRLYRDHYQSHEDGAAFDIKRRSSSASALRAPAASP"
     gene            complement(1025..1963)
                     /locus_tag="VNZ06_11300"
     CDS             complement(1025..1963)
                     /locus_tag="VNZ06_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21384.1"
                     /translation="MSGDAELKIVVNPEGMNADIGVRFLARCFGTCWTEAMYRWYLQR
                     AFGGQSPDRLILMDGERVVASCGLAYRLLRTPDGTVHPVSVVVAACTAPGERGRGCYA
                     RVLRAAVDRSALRGCTAVLGFVTADNATGRGLRRLGAAAVPSAYLASHGVSRVPGAMT
                     LQLRSAKVAAGWPAHASARLRNPPLQAGFHYPDVSDWRAQMVDRPHPVQSLRVGTTCR
                     ALIECVGDIDRLQWLDGDPRERVAAIRAIAAHAHRRKRHFFMYSTRPGDAAVALRLGL
                     IATPGYMMALAPQTRHEPTVRGWATMSWHVQSGDRM"
     gene            complement(1942..>2592)
                     /locus_tag="VNZ06_11305"
     CDS             complement(1942..>2592)
                     /locus_tag="VNZ06_11305"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HWW21385.1"
                     /translation="YLNALPRTDCALHGLNHAHPGPRMAVEFQEQSRSECGALLQEGR
                     QIFATAGLRHVQGFAAPAWNTPPSLCAALSDADFHFVTSARDLDTPVSDRARSAMSGL
                     RGASLIQPMWIRCAPSDKATSASAGPSRLVHFTTNFQATSTIERARDIIETGGLLSIK
                     AHIFKTGGGITMLDGLDAAYCDYLERIWRELDARYGDALWWTSLAEVAGRCRATQS"
BASE COUNT          431 a          830 c          867 g          464 t
ORIGIN      
        1 cctgcttacg gcgctaccgg cacgcattga gaccgtaatg gtcatcgatc cggacgtcaa
       61 aatttgcggt cggcatgagg gcagtgccgt cgagctcgag cgtgtcgtcg ccgatttgca
      121 gcagtcgggg gcagcggcag cgtgcccacg tgtggcgatc gaccgggacg ggtttctggc
      181 gcggttccag gcgctggagt atgccctggc gttcgtggtc gggcgccgaa gtcttgccga
      241 tttcggggtc acgtccggag tgtccattta tagacgcgat gcgctcgaac acgcgctcga
      301 tcagcattca ttgtctatat atgccgagga tctcgagaat gcggtgatcc tgctgcacga
      361 tggagagcgc atctactatg acggtcgcct ggtggtgagc accgaggggc cgggctcatt
      421 gcgtcgctgg ttctcgcagc gggtcggctg gtactacggt ctgctcaggg tttacaccca
      481 gcgtttccac gagctgtggc gtatcgggcg gcgcacgccg tttgcaatgt acaacttcat
      541 cgcctacatg ggcgtgctag ggctgggcct gcatctgctc aggatcgtga gtgccgcgct
      601 attgctcgtg agcgtcgccg ccatcctcga caatctgttc gtgttcaatc tgctgccgaa
      661 cgctggggcg atcaatccgg tgtacttcgc ggctgcgctg ggcagctatc tcgcgctcgg
      721 tgcgctcgca ctgtttgtgg tcgtacccag gagcgagcgc gcctacatag caccaatcgt
      781 gcccatctac ttcctctacg tgttgatgca tgtcgcgccc atcactgtcg gattcgccaa
      841 ctgggtagcg ctgcggctct ggggtcgccg gctctaccgg gaccactacc agtcgcacga
      901 agacggtgcc gctttcgaca tcaagcgccg cagctccagc gcatctgcgc tgcgagcgcc
      961 tgcagcgtct ccatgagcac tgccgtctga accgtcatgt gccgcgatcc ggtgcgtcgc
     1021 ggcgctacat tcgatcaccc gactggacgt gccaggacat cgttgcccag ccgcgtacgg
     1081 tgggctcgtg tcgggtctgc ggcgcgaggg ccatcatgta tcctggggtc gcgatcaatc
     1141 cgagtcggag cgcgaccgcg gcatcgccgg ggcgcgtcga gtacatgaag aaatggcgtt
     1201 tgcggcggtg tgcgtgtgcg gcgatggcgc ggattgccgc tacgcgttca cgcgggtcgc
     1261 cgtcgagcca ctgcaggcga tcgatgtcgc cgacacactc gatgagggcc cggcaggtgg
     1321 ttccgacacg caatgactgc acgggatggg gacgatccac catttgcgct cgccagtcgc
     1381 tcacgtcagg atagtgaaag cccgcctgta gcggcgggtt gcggaggcgc gcgctggcgt
     1441 gcgccggcca ccccgccgcg accttcgcgc tgcgcagctg caatgtcatg gcgccgggga
     1501 cgcgcgaaac gccgtgcgaa gcgaggtacg cgctgggaac cgctgcggcg cccagccgtc
     1561 gcaggccgcg gccggtggcg ttgtcggccg tgacgaatcc aagcaccgca gtacaacccc
     1621 gtagcgcact tcgatcaacc gcggcccgga gcacgcgcgc gtaacagccg cgtccgcgct
     1681 cgccgggcgc ggtgcaggcc gcgaccacaa cgctgaccgg gtgcaccgta ccatccggcg
     1741 tacgcagcaa ccgataggcg agaccacagc tcgccacgac acgctcgccg tccatcagta
     1801 tcaggcgatc ggggctttgg ccgccgaaag cgcgctgcag ataccagcgg tacatagcct
     1861 cggtccagca cgtgccaaaa cagcgcgcga gaaaacgaac gccaatgtca gcgttcattc
     1921 cctcggggtt aacgacgatc ttcagctctg cgtcgcccga catcggccgg ccacctcggc
     1981 gagggaggtc caccacagag catcgccgta gcgggcgtcc agctcgcgcc agatccgttc
     2041 gagatagtcg cagtacgcgg catcgaggcc gtcgagcatc gtgatgccgc cgcctgtctt
     2101 gaagatgtga gccttgatcg agagcagccc cccagtctca atgatgtcgc gcgcgcgctc
     2161 gatcgtggag gtggcctgga agttcgtcgt gaagtgcacg agacggctgg gcccggcact
     2221 ggcgctggtg gccttatcgc tgggggcaca ccggatccac attggctgga tgagagaagc
     2281 gccgcgcagc ccgctcatcg cgctccgggc tcggtcactc accggcgtgt ccaggtcacg
     2341 cgccgaggtg acgaagtgga aatccgcgtc actcagcgcc gcgcacagcg aaggcggcgt
     2401 gttccacgcc ggtgccgcaa agccctggac gtggcgtagc ccggcggtcg caaagatctg
     2461 acgaccctcc tgcagcagcg cgccgcactc gctgcgggac tgttcctgaa actccacggc
     2521 catgcgtgga ccgggatggg catgattcaa gccgtgcagg gcgcaatcgg tgcgcggcag
     2581 ggcgttcaag ta
//
LOCUS       DATMRM010000374         5841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_194944, whole genome shotgun sequence.
ACCESSION   DATMRM010000374 DATMRM010000000
VERSION     DATMRM010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194944"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VNZ06_11310"
     CDS             <1..566
                     /locus_tag="VNZ06_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21386.1"
                     /translation="PLGGYIVPVAYARDIGEKLTLHGIAFEALPEAIEQFEAEHFRAS
                     RVDYSSAPFEGRMRATLSGAWQTRTQRLTAGSLYVPLAQPLARLIVALLEPQAPDSFA
                     AWGFFNACFEQKEQLEPYVAEQVALNMLNGNAALAAEFHARLRDDACFAADPVARLEF
                     FLRRHNCWDERHNLYPIYRVAARPARH"
     gene            721..2196
                     /gene="adh"
                     /locus_tag="VNZ06_11315"
                     /pseudo
     CDS             721..2196
                     /gene="adh"
                     /locus_tag="VNZ06_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            2205..2600
                     /locus_tag="VNZ06_11320"
     CDS             2205..2600
                     /locus_tag="VNZ06_11320"
                     /inference="COORDINATES: protein motif:HMM:NF017426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HWW21387.1"
                     /translation="MSELASDVERVSATADALTLIARLERKHGPLMFHQSGGCCDGSS
                     PMCYPRGDLIVGAQDILLGEIGGHPFYIGAAQFEYWQHTHLIIDVVPGRGGMFSLEGP
                     EGMRFLTRSRLYTDDEARRLEDQPPRPAA"
     gene            complement(2623..3060)
                     /locus_tag="VNZ06_11325"
     CDS             complement(2623..3060)
                     /locus_tag="VNZ06_11325"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HWW21388.1"
                     /translation="MLDLVLACLHHLLIFAIFGLILCEFVMLKPGIDATAIKRISRVD
                     LLYGMTAVLIIVVGFSRAIFAAKGWHYYSHNAWFWAKMGTFGVIGLLSIPPTLALGRW
                     RKSSVVPEQGAIQSVRRLLHYELGLFLLLPLFAAAMARGFGEF"
     gene            complement(3121..4395)
                     /locus_tag="VNZ06_11330"
     CDS             complement(3121..4395)
                     /locus_tag="VNZ06_11330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HWW21389.1"
                     /translation="MNDVTLAAEAPAIRESRTLDRVFRDRILQLLRGLRDCKVIVREG
                     SSSIELGTAAMQLGQTLRTSLRVRDPEFYRRTALNGSVGAAEAYMDGMWDCDDLVGLV
                     RILVRNQERLDAMERGPARLGAALLRMWHSLRRNTRQGSRRNIAAHYDLGNDFFELFL
                     DENLMYSSAVYDSPYESLEVASTRKLDRICHKLQLSADTRVVEIGTGWGGFALHAART
                     TGCHVTTATISREQYNLANARIAAAGLQDRVKVLLADYRELTGRYDRLVSIEMIEAIG
                     HQYLDTYFRKVSSLLEEDGMALIQAITIEDHRYEQALRSVDFIKRYIFPGSFIPSISA
                     MLKSVTGVSNLKLFNLEDIGPSYALTLREWRRRFMAQHDQVRAMGYPERFVRMWEFYL
                     AYCEGGFIERSTGDVQMLLVKPGARRAQFIPN"
     gene            complement(4392..5360)
                     /locus_tag="VNZ06_11335"
     CDS             complement(4392..5360)
                     /locus_tag="VNZ06_11335"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWW21390.1"
                     /translation="MNQTLQPLQRRIATAFARWLDNEAGADDQQNSDRIDWLRTLPFI
                     AVHVACLTVIWVGVSGFAVGVTLALYAIRMFAITGFYHRYFSHRAFRTSRPVQLAFAL
                     IGASSVQRGPLWWAAHHRNHHRFADGDRDPHSPLRLGFFRSHLGWFLTPRGFRTDWKV
                     IRDLASFPELRWLDRFDILMPILLAVALYLLGSYLHGAHPELGTSGFQLVVWGFCIST
                     VALFHATFMINSLAHRWGSRRFSTRDESRNNWLLALITFGEGWHNNHHRFPGAARQGL
                     RWWECDLTFYLLSLMSFLGLIHDLKPYPASMRVRVVPSCEPQGESL"
     gene            complement(5435..>5841)
                     /locus_tag="VNZ06_11340"
     CDS             complement(5435..>5841)
                     /locus_tag="VNZ06_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21391.1"
                     /translation="AVIDAGFDAFVREHWNDLTTPGVAPLPFLVPSELRFVDFSAKKL
                     RDEQIRGRDVSWFRLKLDGWYAFVLPHIDVAYDTQNHDLFEYRGLSNIRGNGNHNLDV
                     TIQFPPEEHRGDLSMPEVQRAAQMPLTGRCAS"
BASE COUNT         1192 a         1836 c         1796 g         1017 t
ORIGIN      
        1 caccgctggg cggctatatc gtgccggtgg cgtacgcgcg ggatatcggc gagaaactga
       61 cactgcacgg catcgccttc gaagctttgc ctgaggccat cgaacagttc gaggccgagc
      121 actttcgcgc ctcgcgggtc gactactcct ctgccccgtt cgaggggcgt atgcgtgcca
      181 cgctgagcgg agcctggcag acacgcacgc agaggctgac cgcgggcagt ctctatgtcc
      241 cgctggcgca acccctcgcc cggctcatcg tcgccctgct cgaaccgcag gcccctgact
      301 ccttcgcggc ctggggattt ttcaatgcct gcttcgagca gaaggaacag ctcgaaccct
      361 atgttgcaga gcaggtggcg ctcaacatgt tgaacgggaa cgccgcgctt gcagcagagt
      421 ttcatgcaag gctgcgagat gacgcctgct tcgccgccga tccggtcgca cggctggaat
      481 tcttcctgcg acgccacaac tgctgggacg agcgccacaa cctctatccg atctaccgcg
      541 tcgccgcgcg tccggcgcgg cactgacgct gtgctcgaac tggcatctcg cgcgcctcgc
      601 ctggctcagc gcaacgcgtt acgatgccgc gcacggggta cgcgcaagcg gcacagataa
      661 tttgaggaga acaacgtgac catcgcacca agcacacacg gcagtttgaa ggtcgcattc
      721 aagacccgct acggcaacta catcggtggc aaatgggtcg agccggtcaa gggcgaatac
      781 ttcgagaaca cgactccaat taccggcgcg cgcttatgcg aaattccgcg ctccacggcg
      841 gaagatatcg agctggcact ggatgccgcg cacgctgcca aggccgcgtg gggcaagact
      901 tcggcggccg aacggtccaa catcctcaac aagattgccg atcggatcga aaccaatctc
      961 aatctgttgg cccatgtgga gacctgggac aacggcaagc ctattcgcga gaccatggcg
     1021 gccgatctgc cgctcgcgat cgatcacttc cgctacttcg cgggttgcat ccgtgcgcag
     1081 gaaggctcac tgtcgcagat cgatcacgac acggttgcct atcattttca cgagccgctc
     1141 ggcgtcgtcg gtcagatcat tccatggaat tttccgctgc tgatggcggt atggaaactc
     1201 gcaccggcac tggcggcagg caactgcgtg gtcatcaagc ccgcggagca gaccccggcg
     1261 tcgatcctgg tactgatgga gctgatctcg gatctgctgc caccgggcgt gctcaacgtc
     1321 gtcaacggat ttggcgtcga agcgggcaaa ccgctggcct ccaacaagcg cgtggcgaag
     1381 atcgcgttca ccggggaaac caccaccggc cgactgatca tgcagtacgc ctcgcagaac
     1441 ctgattcctg tgacgctgga actcggcggc aagtcgccga acgtgttctt cgaggatgtg
     1501 tgcgccaagg acgacgcgtt cttcgacaag gcgctggaag gcttcacgat gttcgccctc
     1561 aatcagggtg aagtgtgcac ctgtccgtcg cgcgcgctca ttcaggactc gatctatgac
     1621 cgcttcatgg agcgcgcgct caagcgcgtc gcagccatca agcaaggcga cccgctgaat
     1681 ccggcgacca tggtcggcgc tcaggcctcc agcgagcagc tgcagaagat tctttcctac
     1741 ctggatattg cgcgacagga aggcgccaaa gtgctggccg gaggcgaacg cgcgaagttc
     1801 gacggcgatc tgggcgacgg cttctacgtg aaacccacgg tgctcgaggg caacaacaaa
     1861 atgcgcgtgt tccaggaaga gatcttcggg ccggtcgtgt cggtaacgac cttcaaggac
     1921 gaggcagagg cgctggcgat tgccaacgac acgctgtacg ggttgggagc cggtgtctgg
     1981 acacgcgatg ccaacaccgc gtatcgcatg gggcgcgcga ttcaggccgg ccgcgtctgg
     2041 accaactgct atcacgccta tccggcgcac gccgcgtttg gtggctacaa gcagtccggt
     2101 gtgggtcgcg agacgcacaa gatgatgctg gatcactacc agcagaccaa gaacatcctg
     2161 atgagctact cacccaaggc gctggggttc ttctaagggc agcgatgagt gagctcgcca
     2221 gtgacgttga acgcgtcagt gccacagctg atgccctgac gctcattgcg cggctcgaac
     2281 gcaagcacgg tccgctgatg tttcaccagt cggggggctg ctgcgatggc agctccccga
     2341 tgtgctatcc gcgtggcgat ctgattgtcg gtgcgcagga catcctcctg ggcgagatcg
     2401 gcggccaccc cttctacatt ggcgcggcgc agttcgaata ctggcagcac acccatctga
     2461 tcatcgatgt ggtccccggg cgcggcggca tgttctcgct cgagggtccg gaaggcatgc
     2521 gctttctgac ccgatccaga ctctataccg acgatgaagc caggcggctc gaggatcagc
     2581 cgccgcggcc tgccgcctga ctgaagaatc ccgaagaatg ctctagaatt ccccgaatcc
     2641 ccgcgccatc gccgccgcaa acagcggcaa cagcaggaac agtcccagct cgtagtgcaa
     2701 tagccgccgc accgactgga tagcgccctg ctcgggcacc acgctggact tgcgccatcg
     2761 acccaatgcc agggtgggtg gaatcgacag caggccgatc acgccgaagg tgcccatttt
     2821 tgcccagaac caggcgttgt gtgagtagta gtgccagcct ttggcggcaa agatggcgcg
     2881 gctgaaccca acaacgatga tcagcacggc cgtcatcccg tacagcaggt caacccgcga
     2941 gatgcgcttg attgccgtcg cgtcgatacc cggtttcagc atcacgaact cacacaggat
     3001 gagcccgaaa atagcgaaga tcagcaggtg atgcaggcag gcgagtacca ggtcgagcat
     3061 gcgcgcatcc tacccgccaa cccggcctcg ttgccagcgc gacgcccgac cggcaagcgg
     3121 tcagttcggg atgaactgcg cgcggcgcgc gcccggcttc accagcagca tctgtacgtc
     3181 gccggtggag cgctcgatga aaccgccctc gcaataggca aggtagaact cccacatgcg
     3241 cacgaagcgc tccggatagc ccatcgcgcg cacctgatcg tgctgcgcca tgaaccgtcg
     3301 gcgccattcg cgcagcgtca gcgcgtagct cggaccgatg tcttcgagat tgaacagctt
     3361 cagattgctg acaccggtca ccgacttcag catcgcgctg attgacggga tgaagctacc
     3421 cgggaagatg taacgcttga tgaaatccac tgagcgcagc gcctgctcgt agcgatgatc
     3481 ctcgatcgta attgcctgga tcagcgccat cccgtcctct tccagcaggc tgctgacctt
     3541 gcggaagtaa gtgtcgaggt attgatggcc gatagcctcg atcatctcga tcgacacgag
     3601 gcgatcatac cggccggtga gctcgcggta gtcggccagc aacaccttga cgcgatcctg
     3661 caagccggct gcggcaatgc gggcattggc gaggttgtac tgctcgcgcg agatggtcgc
     3721 agtggtgaca tggcagccgg tagtccgggc ggcatgcagc gcaaagccgc cccaacccgt
     3781 gccgatctcc accacgcgtg tatcggccga gagctgcaac ttgtggcaga tgcggtcgag
     3841 tttgcgcgtg gaagccactt ccagcgactc gtacggcgag tcatagaccg ccgatgagta
     3901 catcagattc tcatcgagga acagctcgaa gaaatcgttg cccagatcgt agtgcgcggc
     3961 gatgttgcga cggctgcctt ggcgcgtatt gcgacgcagg gagtgccaca tgcgcagcaa
     4021 cgctgcgccc agacgcgccg gaccacgctc catcgcgtcc aagcgctcct gattgcgtac
     4081 caggatgcgc accagcccga ccaggtcgtc gcagtcccac ataccatcca tataggcttc
     4141 ggccgcgccg acgctgccgt tgagcgcggt acgacgatag aactccgggt cgcgcactcg
     4201 caggctggtg cgcaatgtct ggccaagctg catcgcagcc gtgcccaatt cgatcgagct
     4261 cgagccttca cgcacgatga ctttgcaatc gcgcaaaccg cgcagcagct gcaggatgcg
     4321 gtcacggaat acccgatcga gagtccgcga ttcgcgtatg gcaggcgcct cggccgccag
     4381 agtgacatcg ttcacagcga ttctccttgc ggctcacagc tgggtacaac gcgcacgcgc
     4441 atgctcgccg gatacggttt caggtcatgg atgagtccca ggaaggacat caggctcagc
     4501 aggtagaacg tcagatcgca ctcccaccag cgcagcccct ggcgcgcggc gccagggaaa
     4561 cgatgatgat tgttgtgcca accctcgcca aaggtgatca gtgccagcag ccagttgttg
     4621 cggctctcgt cgcgcgtgct gaagcggcgt gatccccaac gatgcgccag cgaattgatc
     4681 atgaacgtgg catggaacag tgccaccgtc gatatgcaga atccccagac gacgagctga
     4741 aagccgctgg tacccaactc cggatgcgcg ccgtgcagat acgaacccag caggtacagc
     4801 gccactgcca gcaatatcgg catcaaaatg tcgaagcggt ccagccagcg cagttccggg
     4861 aagcttgcga ggtcacggat gaccttccaa tcggtgcgga agccgcgcgg ggtcaggaac
     4921 cagccgaggt gactgcggaa gaagcccaac cgcaggggtg aatgcgggtc acggtcgccg
     4981 tcggcgaacc ggtgatggtt gcgatgatgc gccgcccacc agagcggacc acgctgcacg
     5041 ctgcttgccc cgatcagggc gaacgcgagc tgcaccgggc gcgaggtgcg aaacgcccga
     5101 tgggagaagt agcggtgata gaaacccgtg atcgcaaaca tgcggatcgc atacagcgcg
     5161 agggtgacgc ccaccgcgaa gccagacacg ccaacccaga tcaccgtgag gcacgcgacg
     5221 tgcacggcga tgaacggcag ggtgcgcagc cagtcaatgc gatcgctgtt ttgctgatcg
     5281 tcggcaccgg cctcgttgtc gagccagcgc gcgaaggcgg tcgcgatccg ccgctgcagc
     5341 ggctgaaggg tctgattcat cgcctgtcga catccttttt tcaagactct agcggaaata
     5401 cgcccgtcga tgccacgcgg atgcaagggt tacgtcaaga ggcgcaccga cctgtcaagg
     5461 gcatctgggc cgcccgctgc acctccggca tgctgaggtc accacggtgc tcttctggcg
     5521 ggaattggat ggtgacatcc aggttgtggt tgccattgcc gcggatattg ctcaatccgc
     5581 ggtactcgaa caggtcgtgg ttctgggtgt cgtacgcgac atcgatatgc ggcaggacga
     5641 aggcatacca gccatccagc ttcagccgaa accagctcac gtcgcggccg cggatctgct
     5701 catcgcgcag tttcttggcg ctgaagtcga caaatcgcag ctcgctcggc accaggaacg
     5761 gcagcggtgc gacccctgga gtagtcaggt cattccagtg ctcgcgtacg aacgcatcaa
     5821 agcccgcatc gatcaccgca t
//
LOCUS       DATMRM010000375         3380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2064111, whole genome shotgun sequence.
ACCESSION   DATMRM010000375 DATMRM010000000
VERSION     DATMRM010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2064111"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /gene="tldD"
                     /locus_tag="VNZ06_11345"
     CDS             complement(<1..1352)
                     /gene="tldD"
                     /locus_tag="VNZ06_11345"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HWW21392.1"
                     /translation="MSVPETAVPADPLRYAREAILAPAGLDEHRIEQALGLVLGSAID
                     AADLYFQLSHEESWALEDGIVKDGHASIEQGVGVRAMAGEKTGFAYSDEIIMPALEEA
                     ARAARAIAVSRGSGAMQAWQPSAPGHHLYLPVDPRISLKDEDKIGWLERVDRETRRMD
                     PRVSQVMASVHAVHEMILVARSDGVLAGDVRPLVRMNVTVLVEHNGRRERGFAGCGGR
                     YTLDELVAQDRPLALAREAVRQALVNLEAVPAPAGTMTVVIGPGWPGILLHEAIGHGL
                     EGDFNRKGTSAFSGRIGERVAAPGCTVVDDGTLARRRGSLNIDDEGTPTQCITLIEDG
                     VLRGYMQDRMNARLSGTKSTGNGRRESFAHMTMPRMTNTYMLPGPHEPKEILASVQRG
                     IYAVNFGGGQVDITSGKFVFSASEAYLIENGRIGAAIKGATLIGNGPDVLTRVSMVGN
                     "
     gene            complement(1379..2185)
                     /locus_tag="VNZ06_11350"
     CDS             complement(1379..2185)
                     /locus_tag="VNZ06_11350"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HWW21393.1"
                     /translation="MKVAALQMTSGPDVGANLAAARLLLEQARAGGARVAVLPENFSF
                     MGMKSGDKREIAEADGDGPVQQFLSQQARELSLWIIGGTTPLRIERGGRVAAACLVYS
                     DDGRRAARYDKMHLFDVDIPGRTESYRESADIAAGRRPILVPTPAGLLGLSVCYDMRF
                     PELYRQLAAAGAQWLSIPSSFTVPTGQAHWEVLLRARAIENLCYVVAPAQWGHHANGR
                     QTYGDSLIVDYWGVVTARLASGNGVVVAELDLAAQAAARRNFPALTHRVL"
     gene            complement(2182..2781)
                     /locus_tag="VNZ06_11355"
     CDS             complement(2182..2781)
                     /locus_tag="VNZ06_11355"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWW21394.1"
                     /translation="MTEPTAPLLVLASASPRRSELLHQIGVAHRVEPADIDETRKRGE
                     DIDECVQRLARSKALQVQQRLKSLPVLGADTIVVVDDALLGKPRDRADGIEMLRRLSG
                     RSHQVLSAVVLASDRGLHQGISRNVVRFRALSEQECAAYWDSGEPQGKAGAYAIQGLG
                     AVFIEYLSGSYSGVMGLPLYETAQLLKAVGIEIPGPMAR"
     gene            complement(2774..3247)
                     /gene="rlmH"
                     /locus_tag="VNZ06_11360"
     CDS             complement(2774..3247)
                     /gene="rlmH"
                     /locus_tag="VNZ06_11360"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HWW21395.1"
                     /translation="MRLRVMAIGTRMPDWVDSAFNDYWRRMRALWKLELIELPLASRR
                     ESGGATQTAKSAEGRRLLALISPRDFIVTLDEHGTQKSTVELSLWLEQRRASGKDLAF
                     IIGGPDGLAEEVLVRAHLRWSLSRLTMPHALVRVLLAEQIYRATTVLSGHPYHRD"
BASE COUNT          614 a         1184 c         1001 g          581 t
ORIGIN      
        1 tcgttgccga ccatagagac gcgcgtgagg acatccggtc cgttgccgat cagcgtcgcg
       61 cccttgatcg cagcgccaat gcgtccattc tcgatcagat aggcctcgct ggcggaaaaa
      121 acgaattttc ccgacgtgat gtcaacctgc ccgccaccaa aattcaccgc gtagatacca
      181 cgctggaccg aggcaagaat ctccttcggc tcgtgcggac cgggcagcat gtaggtattg
      241 gtcatgcgcg gcatcgtcat atgggcgaat gattcacgac ggccgttgcc ggtggatttc
      301 gtgccgctca aacgcgcgtt catccgatcc tgcatgtagc cacgcagcac gccgtcctcg
      361 atgagggtga tgcattgagt cggggtgcct tcatcgtcga tgttgagcga accgcggcgg
      421 cgcgccagcg tgccatcatc gacgaccgtg catccgggcg ccgccacgcg ctcgccgatg
      481 cgaccgctga atgccgacgt gcccttgcga ttgaagtcac cttccagccc gtggccgatc
      541 gcctcgtgca gcaggatgcc aggccagcct gggccgatca caaccgtcat cgttccggcc
      601 ggagcgggta ccgcctccag attcaccagc gcctgccgta ctgcctcgcg cgccagcgcc
      661 agcggtcgat cctgagccac cagctcatcg agcgtgtagc gcccgccgca accggcgaat
      721 ccacgctcgc gccggccgtt gtgttcgacc agcacggtga cattcatgcg caccagcggc
      781 cgcacatccc ccgccagcac cccgtcgctg cgggcgacca gaatcatctc gtgcacggcg
      841 tgcacgcttg ccatgacctg cgagacgcgt ggatccatgc gtcgtgtctc gcggtcaacg
      901 cgctcgagcc agccgatctt gtcctcgtcc ttgagactga tgcgcggatc aaccggcaga
      961 tataaatgat gcccgggcgc tgacggctgc caggcctgca tcgcaccgct cccgcgcgag
     1021 accgcgatcg cgcgcgccgc gcgtgcggct tcttccagcg ccggcatgat gatctcatcc
     1081 gaataggcga acccggtctt ctcgccggcc atcgcgcgca cgccgacgcc ctgctcgatg
     1141 ctggcgtggc cgtccttgac gatgccatcc tcgagcgccc agctctcctc gtgcgagagc
     1201 tgaaaataca gatccgcggc atcgatggcc gagcccaaca ccagtccgag cgcctgttcg
     1261 atgcggtgct cgtccagtcc cgcgggcgca aggatagcct cacgcgcgta gcgcagcgga
     1321 tcggccggaa cagcggtttc ggggactgac atcggtacct ctcgaggact cctgacgctc
     1381 acaatacccg atgcgtcagg gcggggaaat tgcgcctggc cgcggcttga gcggccaggt
     1441 ccagctcggc cacgaccacg ccgttgccac ttgccagccg cgcggtgacg acgccccaat
     1501 aatccacgat caggctgtcg ccataagtct gtcggccatt cgcgtggtgg ccccattgcg
     1561 ccggtgccac cacataacac aaattctcta tggcgcgcgc ccgcagcagc acttcccagt
     1621 gcgcctgacc ggtgggaacc gtgaacgagg acggaatcga cagccattgc gcccccgccg
     1681 cggcgagctg gcgatacagc tccggaaaac gcatgtcgta gcagacggac agtcccagca
     1741 aacctgcggg ggtcggcacc agtattgggc gcctgccggc cgcgatatcg gcggactcgc
     1801 ggtagctttc ggtgcggccc ggaatgtcca catcgaacag gtgcatcttg tcgtaacggg
     1861 cggcgcgacg cccatcatcg ctgtacacca gacacgccgc cgcgacgcga ccgccgcgct
     1921 cgatcctcag tggtgtcgtt ccgccgatga tccacaacga tagttcgcgc gcctgctgcg
     1981 acaggaactg ctgcaccggt ccgtccccgt ccgcctcagc gatctcgcgc ttgtcgccgg
     2041 atttcatgcc catgaacgaa aaattctccg gcagcacagc aacccgtgca ccgccggcac
     2101 gtgcctgctc cagcagcagg cgcgcggccg ccaggttcgc acccacgtcc gggccggaag
     2161 tcatttgtag cgccgccact ttcatcgagc catcggacct ggaatttcga taccgactgc
     2221 cttcagcaat tgggccgtct catagagcgg caaacccatc actcccgagt agcttcccga
     2281 taggtactca atgaatactg cgcccaatcc ctgaatggcg taagcgccgg ccttgccctg
     2341 aggctcaccg ctgtcccaat aagcggcgca ctcctgctcg ctcaaggctc taaagcgcac
     2401 cacgttgcgc gaaatcccct ggtgcagccc tctgtcgctg gccagcacca ccgccgacag
     2461 cacctggtgc gagcgcccag acaggcgccg cagcatttca atgccgtcgg cccgatcacg
     2521 cggtttgccc agcagcgcat catcgaccac gactatcgtg tcagcgccca gcaccggcag
     2581 cgacttcaac cgctgctgca cctgtagtgc cttgctgcgc gccagacgct ggacgcattc
     2641 gtcgatgtcc tcaccgcgct tgcgcgtctc atcgatatcc gccggctcga cgcgatgtgc
     2701 aacgccgatc tgatgcaaca gttccgagcg ccggggcgat gcggaagcca gcacgagcag
     2761 cggcgcagtc ggttcagtca cgatgatagg gatgcccgga aagcaccgtg gtggcgcgat
     2821 agatctgttc ggcaagcaac acccgtacca gagcgtgggg catggtcagg cgcgacagcg
     2881 accagcgcag gtgtgcccgc accagtacct cttcggccag accatcgggt ccgccaatga
     2941 taaaggccag atccttgccc gatgcgcgcc gctgctcgag ccacagcgac agttccacgg
     3001 tggatttctg cgtaccgtgc tcgtccaacg tcacgatgaa gtcccggggc gagatcagcg
     3061 ccagcagacg ccggccttcg gcactcttgg ccgtctgcgt ggccccacca ctctcacgcc
     3121 gactggcgag gggcaactcg atcagctcca gtttccacag cgcacgcatc cggcgccaat
     3181 aatcgttgaa tgcggaatca acccagtcgg gcatccgcgt tccaatggcc atcacacgca
     3241 gtcgcatgct tgaggcctaa ggttgcgatg ccacccgcgt tgcctaaaat tcgcgtcgcg
     3301 cgcgttactg cgctgccgag cgcgaggagg cgctgtccgg gacaacgctg cgacgtccac
     3361 gttcaggttt ggaggcgggt
//
LOCUS       DATMRM010000376         4377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3606988, whole genome shotgun sequence.
ACCESSION   DATMRM010000376 DATMRM010000000
VERSION     DATMRM010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3606988"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VNZ06_11365"
     CDS             complement(<1..301)
                     /locus_tag="VNZ06_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21396.1"
                     /translation="MIRGRHPRAAPLLLAVAASEGATRDRPDASLVIVIQLYPAIEPY
                     EHGMLEVGDGNRVYWEICGNPRGKVAVVLHGGPGSGCTMWHRRLFDSTVYRVVLFD"
     gene            complement(298..849)
                     /locus_tag="VNZ06_11370"
     CDS             complement(298..849)
                     /locus_tag="VNZ06_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21397.1"
                     /translation="MKKIATLTLLAGCVILAGTRPASAQGNGNSPRIAQVVALDECDP
                     TTFNAALGADECKNVASGFSTMLGNLVAGAEAGTPDPGWDYEPDTLTINSGTVVSVVN
                     QGGEVHTFTEVKAFGNGFIPPLNPGAATTAIPECSGGFGSVAVAKTRILQGSHLDITG
                     LSKGKHLFECCIHPWMRMEVDVK"
     gene            complement(922..1926)
                     /locus_tag="VNZ06_11375"
     CDS             complement(922..1926)
                     /locus_tag="VNZ06_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21398.1"
                     /translation="MKQLNLILVFVVAMFVVCGRCAPALAQEASAAGTLDSQAQDVAA
                     SMKACKPTSTSARCLLTAITTLQAQVTTLQGQVTTLTSQVSSLQTTLTAVQNNHALAL
                     GRYVSVDSSAENGLKGPHIIFSGANVHIESGSGSTVDSSGLGNLVIGYNEDAGSTSII
                     DGNRSGSHNLVVGPQHEFTGSGSVVFGYANFTSANFASVTGGECSAAGLTAYPFVCVD
                     SSGAADAASVTGGFKNRASAFVSSVSGGAVNTASGSGSSVSGGDGNTASGGESSVSGG
                     GGNTASGGESSISGGFVNTASGLDSSVSGGSDNTASGEFSSILGGSDESVTTESGFFP
                     "
     gene            2113..>4377
                     /locus_tag="VNZ06_11380"
     CDS             2113..>4377
                     /locus_tag="VNZ06_11380"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWW21399.1"
                     /translation="MPEDLVQFEDFVLDRGACELRRGDAVVPLQRIPLELLCLLVERR
                     GQLVTREEILERVWGKGVFVDSENSINTAVRKVRRALSDDPDAPRFVATLPGRGYRFL
                     AEVTRAATNGRTVTIPSMPEGPEPIVVGRETELTRLQSWSAQVIEGRRRVVFVAGEAG
                     IGKTTFLKAFLDSLDGSAARIGRGQCVEQYGAGEPYMPVLEALTRLCQESRGQHVVDL
                     LHQFAPTWLAQMPSLLSYADRERLQASTQGVTQQRMLREMTQALEALAAETPLVLALE
                     DLHWSDFSTLELISAIARRTEPARLLILGTYRPTEMLASDHPLRTMKQELELHRNCEE
                     LRLKLLSEADVAAYLAKRFADDNTARSLAGVAPLIHERTDGNPLFMVNVVDYLVEQGS
                     PLNADKIEAPRSILQMIERNLERLNPDEQRVLEAASVVGAEFSAATVAAALERPLSEI
                     EATCTRLSRHEQFVTAHGISEWPDGTVASRFRFHHALYGDVLYGRMPAGHRVELHLRI
                     AEREEKAHGARVGEIAAELAHHYSRANDRNKAIEYFQIAGERAAVRHAMVEAERHYAK
                     ALKLLDELPETPERDRRELALQILLGSAVAAVKGIGAPERERSLLRARDLSERLGEQP
                     HVFPVLWQLFHFHAQRGELQTARECAEHALAVAQSQCDAVMLAYARTALGLCLFYIGD
                     FAACRRQFELAFAQDIPLQKRWYLDERRPDVLAGALSALAEGILGYPDRCCVGVGEVV
                     KRARTQSSLPDLAFA"
BASE COUNT          817 a         1365 c         1327 g          868 t
ORIGIN      
        1 gatcgaacag cacgacgcga tagacagtcg aatcgaacag cctgcgatgc cacatcgtgc
       61 atcctgatcc gggaccacca tgcagcacga cggccacctt gccgcgcgga ttgccgcaaa
      121 tctcccagta cacgcgattc ccgtcgccaa cctcgagcat cccatgttcg taaggttcga
      181 tcgctgggta tagctggatc acgatgacca gactcgcatc tggacggtcg cgtgtcgccc
      241 cttccgatgc agcaaccgcg agcagcagtg gtgctgcccg cggatgccgc ccgcgaatca
      301 cttcacgtcc acttccatcc gcatccatgg atggatgcag cactcgaaca gatgttttcc
      361 ctttgacagg ccagtgatat cgagatggct gccctgaaga attcgggtct tggccaccgc
      421 gacgctgcca aagccgccac tgcattccgg aatggcggtc gtcgccgcac cggggttaag
      481 cggcggtatg aatccgttac cgaatgcctt cacctcggta aaggtgtgaa cctcaccgcc
      541 ctggttgact acggaaacga cggtccccga attgatcgtt agcgtgtcgg gttcgtaatc
      601 ccagcccgga tcgggagttc cagcctccgc ccctgcaact agattgccca gcatggtgct
      661 gaatccagac gcaacgttct tgcattcgtc agcgcccagt gcggcgttaa acgtggtcgg
      721 atcgcattcg tccagtgcca caacttgggc aatgcgcggc gagttaccat ttccctgcgc
      781 actcgcgggt ctcgtgccgg ctagaatcac gcacccagcc agcagagtta gtgttgctat
      841 ctttttcatt acctcgttcc tccttcgaac ttttgaagtg aattattcgg ggggttaatc
      901 ttgcaccccc agccgcgccc gctagggaaa gaaaccggac tcggtggtca cgctttcatc
      961 gctaccgccg agaatgctcg aaaactcgcc gctcgcagtg ttgtcactgc cgccgctgac
     1021 gctcgagtcc aggccgctcg cagtgttgac aaagccaccg ctgatgctcg actccccgcc
     1081 gctcgcagtg ttgccaccgc cgccgctgac gctcgactcc ccgccgctcg cagtgttgcc
     1141 atcgccgccg ctgacgctcg acccactccc gctcgccgtg ttgacagcgc cgccgctgac
     1201 gctcgatacg aaagcgctcg ctcggttctt aaaaccacca gtgacgctgg cggcgtcggc
     1261 agcgcctgaa gaatctacgc acacgaatgg gtaggcagtc aggcccgcag cgctgcattc
     1321 gccgccggtc acactggcga agttggcgct ggtgaagttc gcgtagccga agaccacgct
     1381 gccgctgccg gtgaactcgt gctgcgggcc gaccaccagg ttatgtgacc cgctccggtt
     1441 gccgtcgatg atcgatgtgg accctgcgtc ctcgttatag ccgatcacca ggttccccag
     1501 cccgctgctg tctaccgtgg agcccgaccc gctctcgatg tggacattgg ccccgctgaa
     1561 gatgatgtgc ggtcccttaa gaccgttctc cgcgctgctg tccacgctga cgtagcgtcc
     1621 cagcgccagc gcatggttgt tctgcacggc agttagcgtc gtctggagcg acgagacctg
     1681 tgaggtcaag gtggtgacct ggccttggag ggtggtgacc tgtgcctgga gcgtcgtgat
     1741 cgccgtcagc aggcacctgg ccgaggtact ggtgggtttg caggctttca tggacgcggc
     1801 gacatcctgt gcctgagagt ccaatgttcc cgcggcggac gcctcctgcg ccagcgccgg
     1861 cgcgcatcgc ccgcatacca cgaacattgc caccacgaag accaggatta ggttgagctg
     1921 cttcatgtgc tctccaatta ctcgttcctc ctttttgaag tgaatcatcg gttatcggcg
     1981 cccatcttca ggcatgctga gtcgcttgcc aatggaccgt ggctgaacat ctcctgaact
     2041 ctcgctgaac tcgctgacac gtcgcggcta tgcgcttgcc cacggcgcga gtagaatcgg
     2101 cgacctgaac caatgcccga agatctcgtc cagtttgagg attttgtgct cgaccgcggt
     2161 gcctgtgagc tgcgccgggg tgatgcagtc gttccgctcc aaaggattcc gctcgagctg
     2221 ctatgcctgc tggtcgaacg ccgcggacag ctcgttacgc gcgaggaaat tctcgagcgg
     2281 gtctggggca agggcgtctt cgtcgatagc gagaacagca tcaataccgc ggtacgaaaa
     2341 gtccggcggg cgttgagcga cgatcccgac gcgccgcgat tcgtcgctac cctgccgggc
     2401 aggggctacc gctttctcgc cgaggtcacg agagcggcaa ccaatggcag gacggttacg
     2461 ataccctcca tgccggaggg accggagccg attgtggtcg ggcgcgagac ggagctcacg
     2521 cgattgcaaa gctggtctgc gcaggtgatc gaaggccggc ggcgcgtggt gtttgtcgca
     2581 ggcgaagcgg gcatcggcaa gacaaccttc ctgaaggcct tcctcgattc tctagacggc
     2641 agtgctgcac gaatcgggcg cgggcaatgt gtcgagcaat acggcgcggg tgagccatac
     2701 atgcccgtgc tcgaggcgtt gacgcggctc tgtcaggagt cccgcggtca gcacgtggtc
     2761 gatctgctgc accagttcgc gccgacctgg ctcgcacaga tgccatcatt gttgagctac
     2821 gccgatcgcg agcggctgca agcctcgaca caaggcgtta cccaacagcg gatgctgcgc
     2881 gagatgacgc aggcgctgga ggccctcgcg gcggaaacgc cgctggtgct cgcgctcgaa
     2941 gatctccact ggagtgattt ctcgacgctc gaacttattt cggcaattgc ccggcggaca
     3001 gagcctgcgc gcttgctgat tctcgggacc tatcggccaa ccgagatgct cgcgagcgat
     3061 cacccgctgc gcacgatgaa gcaggagctc gaacttcatc ggaattgcga ggagttgcgg
     3121 ctcaagctgc taagcgaagc ggatgtcgcg gcttatcttg ctaagcgctt tgctgatgac
     3181 aataccgcgc gatctttggc cggggttgcg ccgctgattc acgagcgtac cgatgggaat
     3241 ccactcttca tggtcaacgt ggtcgattac ctggttgagc agggctcgcc actcaatgcg
     3301 gacaagatcg aagcgccgcg cagtattctc cagatgatcg agcgcaacct tgagcgcctc
     3361 aaccctgacg agcaacgagt cctcgaagcc gcgagcgtcg tcggcgctga gttctccgcg
     3421 gcgacagtcg cagcggcact cgaacgtccc ctcagcgaga tcgaagccac ttgcacacga
     3481 ttatcgcgcc acgaacagtt cgtaaccgcg catggaatca gcgaatggcc cgacggaact
     3541 gttgcttcaa ggtttcgctt tcatcacgca ttgtatggcg acgtgctcta cggacggatg
     3601 ccggccgggc atcgtgtcga gttgcatctc aggatcgcag agcgagagga gaaggctcac
     3661 ggggcgcgcg ttggcgagat cgcggcggaa ctcgcgcatc attacagccg cgccaacgat
     3721 cggaacaaag cgatcgaata tttccagatt gcaggcgagc gggccgcggt ccgccacgcg
     3781 atggtcgagg ctgagcgtca ttacgcgaaa gcgctcaagc tgcttgacga attgcccgaa
     3841 acgccggaac gagatcgtcg ggaacttgcc ttgcagatcc tgctcggttc ggccgttgca
     3901 gccgttaagg gaattggcgc gcctgaaaga gagcgcagcc tcttgcgggc tcgcgatctg
     3961 tcagagcgcc tcggagagca gcctcacgtg ttcccggtgc tctggcagct cttccatttc
     4021 catgcacagc gcggtgaact gcaaaccgct cgcgaatgcg ctgaacacgc attggcagtg
     4081 gcccagagtc agtgcgacgc ggtgatgctc gcgtatgctc gaaccgccct cggcctctgt
     4141 ctcttttaca tcggcgactt tgctgcctgc cgcaggcagt tcgagctcgc gttcgcacag
     4201 gacatacctc tgcagaaacg ctggtattta gacgaacgca ggcccgacgt gctggcagga
     4261 gcgctaagcg cgctcgctga gggaattctg ggttatccgg accgatgctg cgtaggagtt
     4321 ggcgaagttg ttaagcgcgc gcggacgcag tcgagcctcc ctgacctggc gttcgcg
//
LOCUS       DATMRM010000377        11130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2065802, whole genome shotgun sequence.
ACCESSION   DATMRM010000377 DATMRM010000000
VERSION     DATMRM010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11130
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2065802"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /gene="mrdA"
                     /locus_tag="VNZ06_11385"
     CDS             <1..1278
                     /gene="mrdA"
                     /locus_tag="VNZ06_11385"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWW21400.1"
                     /translation="HGKNGYREILVNAQGRSVQREGAFAPELHSQAPVAGDDLVLSID
                     LATQQAAEDGLGEHRGAVVALDPNTGDVLALASKPGFDPAAFARGLSRSELAALNDDI
                     QKPLFNRALRGTYPSGSTIKPALGLVALTDHEIAADAKVFCNGVFSLPGSAHLWRADK
                     DEPRGMLDLTEAIARSSDVYFYKLASTMGIDRMDAGLAPFGYGQLTGIDISGEKAGLL
                     PTPAWKERVYKQPWFPGETVNMGIGQGYLLVTPLQLAHIAGVLAERGRSFRPRLVKGL
                     RDTDGNIKWLAAVEEKPVSGISDADWRTVIEAMVHTTHCEPYCGTAWVAFKGAAYDAG
                     GKTGTAQVYTVAQNAKYNAKTLPETLRDHAWFIAFAPAEAPKIAVAVLVENAGFGSSN
                     AAPIARKVMDTYLLRKDTPKEVETATTQARTGD"
     gene            1271..2410
                     /gene="rodA"
                     /locus_tag="VNZ06_11390"
     CDS             1271..2410
                     /gene="rodA"
                     /locus_tag="VNZ06_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017903129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HWW21401.1"
                     /translation="MIDLADTGIARTRRTLFSSAKLLSVLNLDGPLLVGLALLALYGL
                     AILYSASGQSLDTVIRTIMRMALGVAAMLALTHASPNFLRRISPWIYVIGVLLLLIVE
                     VTGVIGKGAQRWLNLGIMRFQPSEIMKLGVPMLAAWYLHDRPLPPDWKSLLMVAVIIF
                     LPVGLVAIEPDLGTAALIAASGALMILMAGLSMRIVLALMVLGVGAAFAGWHVLHDYQ
                     RQRVLTFLNPQNDPLGAGYHIIQSQIALGSGGVFGKGWMHGSQAQLQFLPERSTDFIF
                     AVVGEEFGLIGLLFLLALYLFIVSRAVWLAMQTNDTYSRLLALSIALTFFVYVFINAG
                     MVSGLVPVVGVPLPLVSYGGTSVVTLLAGFGILMGLYSHRKLVGS"
     gene            2410..3444
                     /gene="mltB"
                     /locus_tag="VNZ06_11395"
     CDS             2410..3444
                     /gene="mltB"
                     /locus_tag="VNZ06_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HWW21402.1"
                     /translation="MRRIQWLLATGNVLITLAFSASAQQPPPATPIVEPAHFDLARPD
                     IRAYIEQAVTLGVDRVRLMSLLSAAEPQQKIIDAITKPAEKTLQWWEYRARMLTPLRI
                     EAGATLWREHKELLDRIAVEYQVAPEYLIAVLGVETQYGRVTGRYRVLDALTTLGFDY
                     PTRASYFQSELTQYLLLAREEGFDPLSIRGSYAGAMGALQFMPSSYRKFAVSGRHASH
                     RDLWGDWGDIFASTANYLHQAGWQYGGPVLAEAQLAPDQSVTPPERMVLNETLGGLRS
                     HALTIPSTLPDDTPAVLLAAPQQDAMGYRVGFQNFYVITRYNSSPLYAMAVYDLAQAV
                     KQQLIREAAM"
     gene            3441..4349
                     /locus_tag="VNZ06_11400"
     CDS             3441..4349
                     /locus_tag="VNZ06_11400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWW21403.1"
                     /translation="MRSRRLYGGLSLLLLAGCFSPPARRTPPAPTPAPTAPSTVLPSN
                     PPSVNDIPDAIPRFEPRSVYGNPPFYVVAGHRYAVLPSSAGYVERGVASWYGTEFHGL
                     KTSTGESYDMFAMTAAHKTLPLPCYARVTNLSNGRSAVVRINDRGPFVANRIIDLSYS
                     AARKLDMIRNGTAFVQLEVLTPAAPMVTAELPVSVPAISASVIGASTAPPIPGPPPAV
                     PPPAALPSSTFFIQVGAYAKSQNAQSVARRLRASGIDRVITLAPTDAQPLQRVRIGPI
                     VSVQQFDALISQLATLGYPNARLAQD"
     gene            4386..5618
                     /locus_tag="VNZ06_11405"
     CDS             4386..5618
                     /locus_tag="VNZ06_11405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HWW21404.1"
                     /translation="MRSRRYGLALALGLSLLLPATLMAKAKPAHPKPHAAAAASRIEG
                     TAVAGVTETPGVPNPPEVFAKAYILIDYASGQVLAQHDADARMEPASLTKLMSCYAVF
                     HALKAGTLKLQDSITISEHAWRAEGSRTFVQVGSQVPAEILIKGMIVQSGNDASIALA
                     ERIGGTEPAFVQMMNDYAKQLGMKNTHFDDSTGLPSPTHYTTARDLALLATALVRDFP
                     EYYTWFSIKEFVWNNIKQDNRNGLLERDPTVDGLKTGHTDSAGYCLVTSAKRDTMRLI
                     GVVLNSPSIKGREDASAALLNYGFTFYQTVTVKPKDSVILKPRVYKASEEYFSIGPAS
                     DVIMTLPRATAGSIQTTANAQRRLIAPIPKTTPVGELQIVINDKPVKSVPLFPLADVP
                     AGGIWSRLSDTVVLWFQK"
     gene            5623..6477
                     /locus_tag="VNZ06_11410"
     CDS             5623..6477
                     /locus_tag="VNZ06_11410"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HWW21405.1"
                     /translation="MAEPLPICYLNGKLLPLTQARISPLDRSFLFGDGVYEVIAVRHG
                     LARRLPGNLARLERSLNELRILNPHTPAQWGEIVARLIRANGAGNLYVYLQVSRGAEL
                     GRNHAPLPAVEPTVFGFCAPLPLPTPEALERGVACITAVDTRWARCDIKSVALLANVL
                     LRQKAVEAHASETILLRDGMLTEASASSVYVISGGRICTPPNSNLLLPGTTRTLVEEL
                     ADQAGISRHVDGVTEVELRCADEVWLSAATRGVVAVTSIDGNPVADGKPGPLWQRMHE
                     LVEAYWAP"
     gene            complement(6489..7844)
                     /locus_tag="VNZ06_11415"
     CDS             complement(6489..7844)
                     /locus_tag="VNZ06_11415"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWW21406.1"
                     /translation="MTPSQARAELEQPSTPSDPLQSWWMPFTHNRHFKAHPRLIESAS
                     GAYYTLAGGRRVFDCLSGLWCTPLGHAHPKIVHAVQQQVQRLDFSPGFQMGHRAAFEL
                     AAKIAALAGRPSDRVFFVNSGSEAVDTALKIAVAYHRARGEASRTRIIGRERGYHGVG
                     LGGISVGGIPANRKMFAPLMIPGVDHLPHTYNAAEMRFSRGQPQWGAHLAEELERLVA
                     LHDASTIAALIVEPMQGSAGVIVPPRGYLERLREICTRHGILLIFDEVITGFGRLGTP
                     FASQYFNVAPDLTTFAKAVTNGVVPMGGVLTRAGIYDAFMTGPEHAIEFFHGYTYSGH
                     PLAVGTALAAIESLLDEKLIERSAQLAKVLEEAIHTLRDEPHVADIRNFGLAAAVELE
                     SQPGLAGMRGMRVLERGLDEGILLRVTGDTIAVAPPFIASVEEVKAMVESLRRTLRGL
                     S"
     gene            7977..10508
                     /locus_tag="VNZ06_11420"
     CDS             7977..10508
                     /locus_tag="VNZ06_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWW21407.1"
                     /translation="MTVRTAMISHPGQLAAGLALGLLASTHPVHAADENGTAAASEEI
                     ATLQEVVVTSTRREENLSKVPISVTALTQDQLDTKGIKDITDVARFTPGINVDNSGTG
                     AVAIRGIASSGGAGTTGIYIDDTPIQIRALAFNPDETLPKSFDIDRIEVLRGPQGTLF
                     GAGSEGGTVRYITTQPSLTQTTIYSRAEASYTEGGEPSYEAGVAAGGPIIDGTLGARA
                     TVWYRRDGGWIDRVDPTAPNPQSAIVDSNVNHGETVLVRLAAVWAPAGAWKITPSLYY
                     QNRQLHDADTYWPIYSNPIRDKYVSGAPQAIPEPDKFYLPSLKIDGDLGPVELISNTS
                     YYHRKDTTGYDGTLYNLGFYQLYPQSGFLDIAGNPLAFLPGTQFPLLDGTGLHLPASI
                     ANYRSPATVDNDQQNLTQEIRLLSNNKDAKFIWTAGVFFSVNHQQYLEQIHDPLLNNF
                     WNALVGEGYTNIFFYTDNNGNNVPVTYNAAFPNDAYFLQTHATDQQIAVYGEGTYAIT
                     DTLKATVGARYSNLRFYFNTLTGGPQLFLPNQINSGDKRENSFTPKVNISWQADPNNL
                     FYATYSKGFRPGGANNPVPQVACSTDFANFGITAAPSTYSSDTVDSYEIGAKNNFGNR
                     LKIASSIYYIQWHNIQQRIVPPVCQISFIANLGQAAVKGADFQSEFAFTERFTGELTA
                     GFTEARFTKNSVFTANLVPGATPAPIVQSGDAIIGESGQPVSPVTASAGLEYHFRAFD
                     RESFWRADYEYTSHSRWTPPTQDPNTQLFDPANFTLEDTHFVSLRAGMKFGEMQLEPF
                     IDNLFDTHTVTNYSFSVYPGPGISRLESDFTFRPRTFGITFTYRH"
     gene            10811..>11130
                     /locus_tag="VNZ06_11425"
     CDS             10811..>11130
                     /locus_tag="VNZ06_11425"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWW21408.1"
                     /translation="MSASLPRVAIVGATGAVGTELIACLQQRQFGLSDLRLFASERSA
                     GKRMPYANESLSVETLSEDAFRRSDIALFCAGGARARQFAPFAVRAGAIVIDNSSAFR
                     MDSDV"
BASE COUNT         2120 a         3658 c         3336 g         2016 t
ORIGIN      
        1 catggcaaga acggctaccg tgagatcctg gtcaacgctc aggggcgctc ggtgcagcgc
       61 gaaggcgctt tcgccccgga gctgcattcg caggcgccgg tggccgggga cgacctggtc
      121 ctgtcgatcg atctggccac ccagcaagcg gccgaagatg gcctgggcga acatcgcggc
      181 gcggtggtag ccctcgaccc caacaccggc gatgtgctgg cgctagccag taaacccggt
      241 ttcgatccgg ccgctttcgc gcgcggtctg tcgcgcagcg agctcgccgc gctgaacgac
      301 gacatccaga agccactgtt caaccgcgcc ctgcgcggta cctacccttc cggatccacc
      361 atcaagccgg cgctggggct ggtggcactg accgatcacg agatcgcagc ggacgccaag
      421 gtgttctgca acggcgtatt tagtctgcct ggcagcgctc acctgtggcg cgccgacaaa
      481 gacgagccgc gcggcatgct cgacctgacc gaggccatcg cccgctcctc ggacgtctac
      541 ttctacaaac tcgcctcgac catgggcatt gataggatgg acgcgggact ggccccgttc
      601 ggctacggac agctcaccgg tatcgacatc agcggcgaga aggcgggctt gctgccaacg
      661 ccggcctgga aggaacgcgt ctacaagcag ccctggttcc ccggagagac cgtcaatatg
      721 ggtatcggtc agggctatct gctggtgaca cccctgcagc tcgcgcatat cgccggggtg
      781 ctggccgagc gcggccgcag cttccgtccg cgcctggtga aggggttgcg cgacaccgac
      841 ggcaacatca agtggcttgc cgccgtcgag gaaaagccgg tcagcggcat ttccgatgcc
      901 gactggagga ccgtgatcga ggccatggtt cataccacgc actgtgaacc gtattgcggc
      961 actgcctggg tcgccttcaa gggcgccgcc tacgatgccg ggggcaagac cgggacagcg
     1021 caggtgtaca ccgtggcgca gaacgccaaa tacaacgcca agacgctccc ggaaacgctg
     1081 cgtgaccatg cctggttcat cgcctttgca ccggccgagg ccccgaagat tgcggtagca
     1141 gtgctggtgg aaaatgcggg gttcggctcg agcaacgccg caccgatcgc gcgcaaagtg
     1201 atggacacgt acctgctgcg caaagacacg ccgaaggaag tcgaaaccgc taccacgcag
     1261 gctcgcaccg gtgattgacc tcgctgatac cggaatcgca cgcacacgcc gtacgctgtt
     1321 ttcctcagcg aagctgctgt ccgtgctcaa cctcgacgga ccgctgttgg tgggcctggc
     1381 gctgctcgca ctctacggcc tggccattct ctacagcgcc tccggacaga gcctggacac
     1441 ggtgattcgc accatcatgc gcatggcgct cggggtggcg gccatgctgg ccctgactca
     1501 tgcgagcccc aacttcctgc gccggatctc cccgtggatc tatgtgatcg gcgtgctgtt
     1561 gctgctgatc gtggaggtca ccggtgtcat cggcaaggga gcgcagcgct ggctcaacct
     1621 cggcatcatg cgttttcagc cctccgagat catgaagctg ggtgtgccga tgctcgcggc
     1681 ctggtatctg cacgatcgtc cgctgccgcc ggactggaag tcgctgctga tggtcgcggt
     1741 catcattttt ctgccggtgg gcctggtggc gatcgagccg gatctgggta cggccgcgct
     1801 gatcgcggcg agcggcgcgc tgatgattct gatggcggga ctgtcgatgc gaatcgtgct
     1861 ggcactgatg gtgctgggtg tcggcgccgc tttcgccggc tggcacgtgc tgcacgacta
     1921 tcagcgccaa cgcgtactga cgttcctcaa tcctcaaaac gatccgctcg gggcgggcta
     1981 tcacattatt caaagccaga tcgcgctcgg atcgggcggc gtgttcggca agggctggat
     2041 gcatggcagt caggcgcagc tgcagttcct gcccgaacgt tccaccgatt tcatcttcgc
     2101 cgtcgttggc gaggagttcg ggctgatcgg cctgctgttc ctgctggcgc tgtacctgtt
     2161 catcgtctcg cgcgccgtct ggctcgcgat gcagaccaac gacacctatt cacgcctgct
     2221 cgctctgagt atcgcgctga ccttctttgt ctatgtgttc atcaacgccg gcatggtgag
     2281 cggtctcgtg ccggtcgtcg gtgtgccgtt gccgcttgtc agttatggtg gaacatcagt
     2341 cgtgaccctg ctggcgggct tcggtattct gatgggcctt tattcacatc gtaaactcgt
     2401 cggatcctga tgcgtcgaat acagtggctg ctggcaacgg ggaacgtttt gatcacgctc
     2461 gcattcagcg cgagtgccca acagccacca cccgcgacgc cgatcgtcga gccggcgcat
     2521 tttgatctgg cgcgcccgga tatccgcgcc tatatcgaac aggctgtcac gctcggagtg
     2581 gaccgagtac ggctcatgtc gctgctgtcg gccgccgaac cgcagcagaa gatcatcgat
     2641 gcgatcacca agccggccga gaaaacgctg caatggtggg aatatcgcgc acgcatgctg
     2701 acgccgcttc gcattgaagc cggcgcgaca ctctggcgtg agcacaagga gctgctcgat
     2761 cggatcgcgg tcgaatacca ggtggcaccc gagtacctga tcgcggtgct gggggtcgag
     2821 actcaatacg gacgggtgac cggacgctac cgggtgctcg atgcgctgac cacactcggc
     2881 ttcgactacc ccacccgcgc cagctatttc cagagcgaac tgacacagta cctgctgctg
     2941 gcgcgcgaag aaggcttcga tccgttgagc atccgcggct cgtatgccgg tgccatgggg
     3001 gcattgcagt tcatgccatc gagctatcgc aagtttgccg tcagcggtcg ccacgcctcg
     3061 caccgggacc tgtggggcga ctggggggac atcttcgcca gcaccgccaa ctatctgcat
     3121 caggcaggct ggcaatacgg tggcccggtt ctggctgaag cgcagctcgc accggatcaa
     3181 tcggtcacgc cacctgaacg catggtgctc aatgaaaccc tcggcggcct gcgctcgcac
     3241 gcactgacga ttccgagcac cctgccggat gacacgcctg ccgttctgct ggccgctccg
     3301 cagcaggatg cgatgggtta tcgcgtcggc ttccagaact tctacgtgat cacacgctac
     3361 aacagcagtc cgctgtacgc gatggccgtc tacgatctgg cgcaggccgt gaagcaacag
     3421 ctcatccgcg aggcggcgat gtgagatcgc gcaggctgta tggcggtctc agcctgctgc
     3481 tgctcgcggg ctgtttcagc ccgcctgcgc gtcgcacgcc gcctgcgccc acgccggcac
     3541 cgaccgcccc gagcacggta ctgccgagca acccgccaag cgtcaacgac atcccggacg
     3601 cgatacctcg cttcgagccg cgcagtgtgt atggcaatcc gcccttctac gtggtcgccg
     3661 gtcaccgtta cgccgtgctg cccagctcag ccggatacgt cgaacgcggt gtcgcctcct
     3721 ggtacggaac tgagtttcat ggtctgaaga cctccaccgg cgagtcgtac gacatgttcg
     3781 ccatgaccgc cgctcacaaa acgctgccac ttccgtgcta cgcgcgcgtg accaacctat
     3841 caaacggccg cagcgcggta gtgcgcatca acgaccgcgg gccgttcgtc gccaatcgca
     3901 tcatcgatct gtcgtactcc gcggctcgca agctcgacat gatccgcaac ggcaccgctt
     3961 tcgttcagct cgaggtgctg acgccggcag cgccgatggt caccgccgag ctgccggtct
     4021 ctgtaccggc gatctccgct tcggtcatcg gcgccagcac tgcgcccccg atcccgggcc
     4081 cgccgcccgc ggtaccaccg ccggccgcgc taccgagcag cacgtttttc attcaggtag
     4141 gagcctacgc caagtcgcag aacgcccaga gcgttgcgcg cagactgcgc gccagcggca
     4201 ttgatcgtgt gatcacgctg gccccgaccg atgcgcagcc tctgcagcgc gttcgcatcg
     4261 gtccgatcgt cagtgtgcaa cagttcgacg cgctcatctc gcagctcgcg accctgggat
     4321 atccgaacgc gagactcgca caggactgaa ttcagccgct acacttgcga cccatgaaaa
     4381 tgaaaatgag atcacgtcga tacggacttg ccctggcgct gggactgtcg ctgctgttgc
     4441 cagcgacgct gatggccaaa gccaaaccgg cgcacccgaa accgcatgcc gccgctgcgg
     4501 cctcgagaat tgagggaacc gccgttgccg gcgtcaccga aacccccggc gtgcccaatc
     4561 cgccagaggt ctttgccaag gcgtacatcc tcattgacta cgccagcgga caggtgctgg
     4621 cgcagcacga tgctgatgcc cgcatggagc ctgcgagcct caccaagctg atgagctgct
     4681 atgccgtgtt tcatgccctc aaggccggaa cactcaagct gcaggactcg atcacgatca
     4741 gcgagcatgc gtggcgggcc gaggggtcac gcactttcgt gcaggtggga agccaggtac
     4801 ccgcggagat actcatcaag ggaatgatcg tgcagtccgg caacgatgcc agcattgcgc
     4861 tggccgagcg tattggtggc accgagccgg cgtttgtaca gatgatgaac gactacgcca
     4921 aacagctcgg aatgaagaac acgcatttcg acgacagtac cggactgccc tcgcccacgc
     4981 actacaccac cgcgcgcgac ctggcattgc tggcgacggc actggtccgc gactttcccg
     5041 agtactacac ctggttctcg atcaaagagt tcgtctggaa caacatcaaa caggacaacc
     5101 gcaatggcct gctggagcgc gatcccacgg tggatggtct gaagaccggg cacaccgaca
     5161 gcgccggcta ttgcctggta acctcggcca agcgcgatac catgcgcctg atcggcgtgg
     5221 tgctgaattc accgagcatc aagggccgcg aggacgcgag cgcggcgctg ctcaactacg
     5281 gttttacgtt ctatcagacc gtcacggtca agccgaagga cagcgtgata ctgaagccgc
     5341 gcgtgtacaa ggcatccgag gagtacttca gcatcggacc ggccagcgac gtcatcatga
     5401 cgttgccgcg tgccacggct ggcagcattc agaccaccgc caacgcgcag cgccgactga
     5461 ttgccccgat accgaagacg acgccggtgg gcgaactgca gatcgtcatc aacgacaaac
     5521 cggtgaagtc cgtgccactg tttccgctgg ccgatgtgcc tgccggtggt atctggagcc
     5581 ggttgagcga cacggtcgtg ctctggtttc agaagtagcc ccatggccga gccgctgccg
     5641 atctgctatc tgaacggcaa gctgctaccg ctcacgcaag cgcgcatatc cccgctcgat
     5701 cgaagcttcc tgttcggcga tggtgtctat gaggtcatcg ccgtgcgtca cgggctcgcg
     5761 cgcaggctcc ccggcaacct ggcacgtctc gagcgcagcc tgaacgagct gcgcattctc
     5821 aatccgcaca cgcccgcgca atggggcgag atcgtcgcca gactgatccg cgccaatggc
     5881 gctggcaatc tctatgtcta cctccaggtg tcgcgcggcg ccgaactggg ccgcaaccac
     5941 gcgccgctgc cggcggttga acccacggtg ttcggcttct gtgcacccct gccgctgccc
     6001 acgcccgaag cgctcgagcg cggcgtcgcc tgtatcaccg cggtggatac gcgctgggcg
     6061 cgctgcgaca tcaaatcggt ggcgctgctg gccaatgtgt tgttgcgcca gaaggcggtc
     6121 gaagcccatg ccagcgaaac catcctgctg cgtgacggca tgctgaccga ggccagtgcc
     6181 tccagtgtct acgtgatcag cggcggtcgc atctgtacgc cgcccaattc caatctgcta
     6241 ttgcctggca ccacgcgtac gctggtcgaa gagctggcgg accaggctgg catttcccgc
     6301 cacgtggacg gcgtcaccga ggttgagctg cgctgcgccg atgaggtgtg gctatcggca
     6361 gccacgcgcg gcgtggtcgc cgtcacgtca atcgacggca atccggtcgc cgatggaaag
     6421 cccggaccac tctggcagcg catgcacgag ctggtcgagg cctactgggc gccttgaagc
     6481 gccaaccgtc aggacagccc ccggagcgtg cgtcgcaacg attcgaccat tgccttcacc
     6541 tcctcgacgc tcgcaatgaa cggcggcgcc acggcgatgg tatcgcccgt cacgcgcagc
     6601 agaatcccct catccagacc gcgttccagt acacgcattc cgcgcatgcc ggcgagtcct
     6661 ggttgggact ccagctcgac cgcggccgcg agaccgaagt tgcggatatc ggcgacgtgc
     6721 ggctcgtcac gcagtgtatg aatcgcctcc tccagaactt tggccagctg agccgaacgc
     6781 tcgatcaact tctcgtccag caagctctcg atggccgcca gagcagtgcc gaccgcgagc
     6841 gggtgtcctg aataggtgta gccatggaag aactcgatcg cgtgctccgg gccggtcatg
     6901 aacgcatcgt agatcccggc gcgcgtcagc accccaccca tcgggaccac gccgttggtg
     6961 accgctttgg cgaacgtcgt cagatcgggc gcgacattga agtactgtga agcaaacggc
     7021 gtacccagcc gcccaaagcc ggtaatgacc tcatcgaaga tcagcaggat gccgtgacgc
     7081 gtgcagatct cgcgcagccg ctccagatag ccgcgcggcg gaacgatgac gcccgcagaa
     7141 ccctgcatcg gctcaacgat gagcgccgca atggtggacg catcgtgcag cgccaccagc
     7201 cgctcgagct cttccgccag gtgggccccc cactgtggct gaccgcggct gaagcgcatc
     7261 tcggcagcgt tgtaagtgtg cggcaggtga tcgacgccag ggatcatcag tggcgcaaac
     7321 attttccggt tggcgggaat tccaccgacc gatataccgc cgagccccac gccatgatag
     7381 ccgcgctcgc gtccgatgat gcgggtgcgc gaagcctcgc cccgcgcacg atggtaggcc
     7441 acggcgatct tcaatgccgt atcgaccgcc tccgagccgg aattgacgaa gaacacccga
     7501 tcgctcggac gacccgcgag cgcggcgatc ttggccgcga gctcaaacgc cgccctgtgc
     7561 cccatctgga aaccgggact gaagtccagg cgctgcacct gctgctggac cgcatgcacg
     7621 atttttggat gcgcgtgccc tagcggagtg caccacagac ccgataggca atcgaacacc
     7681 cgcctgcctc cggcaagcgt gtagtaagcg cccgatgcgc tttcgatcag acgcggatgc
     7741 gccttgaaat ggcggttgtg cgtaaacggc atccaccacg actgcagcgg atcgctgggt
     7801 gtggatggct gctcgagttc ggcgcgtgcc tgagaaggtg tcatagaggg cccccggtgc
     7861 gaactacgtt accatgagcg catgatgcaa tgttttgccc accgctcgcg gcttcgccta
     7921 tgattctagc gcgaaagcct acgacgatcg ccgcacgcga taagcaaaaa gggagaatga
     7981 cagtgcgcac cgcaatgatc agccaccccg gccagcttgc tgccggactg gccctaggac
     8041 tgctggcgag cactcacccg gttcatgcgg cggatgagaa cggtaccgcc gccgcgagcg
     8101 aggaaatcgc cacgttgcag gaagtcgtgg tcacctccac ccgtcgtgaa gaaaacctga
     8161 gcaaagtgcc aatcagcgtt accgccctga cgcaggacca gttggacacc aagggcatca
     8221 aggacatcac cgacgtcgcg cgcttcacgc cgggtatcaa tgtcgataac tccggcactg
     8281 gcgccgtcgc gatccgcggc atcgcctcct ccggtggcgc cggaacgacc ggtatctata
     8341 tcgacgacac gccgatacag atccgcgcgc tggcattcaa tcccgatgag acactgccaa
     8401 agtcattcga catcgaccgc atcgaggttc tgcgaggccc gcagggcacg ctgttcggtg
     8461 ccggatcaga aggcggcacg gtgcgttaca tcaccactca gccgagcctg actcagacca
     8521 ctatttacag tcgcgctgaa gcgtcgtaca ccgagggcgg tgagccgagc tatgaggccg
     8581 gtgtcgccgc gggcggtccg atcatcgacg gcacactcgg cgcacgcgcc accgtctggt
     8641 accggcgtga tggcggctgg atcgatcggg tcgatccgac cgcgcccaat ccgcagagcg
     8701 cgatcgtcga cagcaacgtc aatcacggcg agacggtgct ggtgcggctg gcagccgtct
     8761 gggcgccggc cggagcctgg aagatcaccc cgagcctcta ctaccagaac cgccagcttc
     8821 acgatgcgga tacctattgg ccgatctatt ccaatcctat cagggacaaa tacgtcagtg
     8881 gcgctccgca ggcgatcccg gaaccggaca agttctacct gccctcattg aagatcgacg
     8941 gcgatctcgg tccggtggag ttgatctcca acacctcgta ttaccatcgc aaggacacta
     9001 ccggttacga cggcacgctg tacaacctcg gtttctatca gctctatccg cagagtggct
     9061 tcctggatat tgcgggaaat cctctggcct tcctcccggg aacacagttc ccgctgctgg
     9121 acggcaccgg cctgcatctg ccggcctcca tcgccaacta tcgctcaccg gcgaccgtgg
     9181 acaatgatca acagaacctg acgcaggaga ttcgtctgct gtcgaacaac aaggacgcga
     9241 agttcatctg gaccgccggt gtgttcttct cggtcaatca ccagcagtat ctggagcaga
     9301 tccacgatcc gctgctgaat aatttctgga acgcgctggt cggtgagggc tacacgaaca
     9361 tcttcttcta caccgacaac aacggcaaca acgttccggt cacctacaac gcggcgtttc
     9421 cgaacgatgc ctacttcctg cagacacacg ccaccgatca gcagatcgcg gtctacggcg
     9481 aaggcaccta tgcgatcacc gatacgctga aggcgacagt tggcgcgcgc tattcgaatt
     9541 tacgcttcta cttcaatacg ctgaccggtg ggccgcagct gtttctgccg aatcagatca
     9601 acagcggcga caaacgcgaa aactcgttta cgcccaaggt gaatatttcc tggcaggcag
     9661 acccgaacaa tctgttctat gccacttatt ccaagggctt ccgacccggc ggcgccaaca
     9721 acccggtgcc gcaggtcgcg tgctccacgg attttgcgaa cttcggcatc acggcagcgc
     9781 cgtcgaccta tagctcggac accgtggaca gctacgaaat cggtgccaag aacaatttcg
     9841 gcaaccgcct gaagatcgcg agcagcatct actacattca gtggcacaac attcagcagc
     9901 gcatcgtgcc gccggtgtgc cagatctcgt tcatagcgaa tctcggacag gccgccgtca
     9961 aaggcgcgga cttccagagc gagttcgcct tcaccgagcg cttcaccgga gaattgacgg
    10021 caggctttac cgaggcccgc ttcaccaaga actcggtctt caccgccaat ctggtgcccg
    10081 gcgctacacc cgccccgatc gtgcaaagcg gcgatgccat catcggcgag agcggtcagc
    10141 cggtatcgcc ggtgaccgcc tcggctggcc tcgagtatca cttccgggcc ttcgatcgcg
    10201 agtccttctg gcgtgcggac tatgagtaca ccagtcactc acgctggacc ccgccgacgc
    10261 aggaccccaa cacacagctg ttcgaccccg ccaacttcac gttggaagat acccatttcg
    10321 tgtcgctgcg tgcgggcatg aagtttggcg agatgcaact cgaacccttc atcgacaacc
    10381 tgttcgacac gcacaccgtc acgaactaca gcttctcggt ctatccggga ccaggcatca
    10441 gccggctgga gtcagacttc acgttccggc cacgcacgtt cggcatcacg ttcacctacc
    10501 ggcactgaca tccggcctgc gtcgccagca cgccatttaa ctgtacgccc cctggactgt
    10561 gatgccagca gacatcgcct gcttcgaggc tttaccagca atgcgtcatt cgcatatgct
    10621 gtcgcgcatg ctgctcgtct gcctgaccac gaccaaaacc aaaacccgcg ccgtcggggc
    10681 ggttcggtcg tgtccgtgtt gagaccagca acataaggtt cacccgaaag gccccgccgg
    10741 aaaggacggg gccttttggt tttgacgctc cttcctccgg tccagcatcg aattgaagga
    10801 gtagtgagtc atgtccgcat ctcttccacg tgtcgcgatc gtcggtgcca ccggcgcggt
    10861 cggcaccgaa ctcatcgcct gcctgcagca gcgtcaattc ggcttaagcg atctgcgact
    10921 gtttgcctcg gagcgctcgg ccggcaaacg catgccgtac gcgaacgagt cgctgagcgt
    10981 ggagacgctg agcgaggatg ccttcagacg tagcgatatt gcgctgttct gcgctggcgg
    11041 cgcacgcgcg cggcagtttg caccgttcgc ggtacgggcc ggggcgatcg tgattgacaa
    11101 ctcctcggcc ttccgcatgg acagcgacgt
//
LOCUS       DATMRM010000378         3046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2066219, whole genome shotgun sequence.
ACCESSION   DATMRM010000378 DATMRM010000000
VERSION     DATMRM010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066219"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VNZ06_11430"
     CDS             complement(<1..237)
                     /locus_tag="VNZ06_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21409.1"
                     /translation="MIVHVMAALPLIAWLYLLLARAGFWRVSPALLPTKRPPDTAVGV
                     VAVIPARDEVLTIGDTVASLLRQDFAGSIQVIVVD"
     gene            268..2499
                     /locus_tag="VNZ06_11435"
     CDS             268..2499
                     /locus_tag="VNZ06_11435"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWW21410.1"
                     /translation="MRRICRVLFLLMPLSGMSSELTIERIFADPALSGPTPRAVKVSP
                     DGRRVGLLRGRPTDQHQLDLWTYDVKDASMQMRVDSKRLAPTEQLSDAERARRERERI
                     ADFHGIVNYDWAPDSHQVLFTLNGNLYLYDLDAPAATALRQLAHGAQAIVDPQVSPRG
                     RYVSFVREQNLWVIDLNSGAEHQLTTDGGGAVHNAEAEFIAQEEMGQTSGYWWAPDDS
                     MIAYKRFDESGVPIARRFEVYAERIDVVEQRYPAAGDPNVSVKLGLVAPTASATRWID
                     LGSNADIYLVRADWTPDARQLAFQRLSRDQKRLDLVLVAADTLKETGLVAELSNTWIN
                     LSDDLHFLKRQRALIWSSERTGSKHLYLIDLDGHVRYPLTAGAWHVDDLLAVDEPAGL
                     LYFAANKDAVIDAQIYTARLDGRDPSHPNRVSQGDGWHAAQFSRQAPRVALYVDTYSD
                     PTTPPEVSINAPDGHRLAWIEANPLDSNHPYWPYHERHVAPEFGHIQAEDGQELEYAL
                     MKPPRFDPSRRYPVFLPVYGGPTVQRVQRHWPDLINEYMAQRGYIVFELDNRGSSRRN
                     RAFQDPIHLRLGEIEARDQLAGARWLQTQAWVDPKRIGVFGWSYGGYMTLMLLAKGSN
                     VIAAGAAVAPVTDWLLYDTCYTERYLGMPKDNSLGYRQSSVFSALDAMTSPLFLAHGM
                     ADDNVLFVNSTQLMSELQRREVQFELMTYPGGKHGLSTPQMKTHVYSAIQRFFDTHLK
                     AGS"
     gene            complement(2565..>3046)
                     /locus_tag="VNZ06_11440"
     CDS             complement(2565..>3046)
                     /locus_tag="VNZ06_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21411.1"
                     /translation="NSVVAVATLDASKSFGPSAFGPLQFRVVANGTAGDWQPLVTLVR
                     LPSLRELQCPATPELACKLSGSNLFLVDAVSSDAQFGHPVQVPDGFPGYTLPVPRPTD
                     GQLYVRLRDDPSVVNQAILVAQELPASAEEAARAAARHAAANHTDASTSAATVSSPD"
BASE COUNT          603 a          951 c          932 g          560 t
ORIGIN      
        1 gtccacgacg atcacttgga tcgagccggc gaagtcctgg cgtaacaggg atgcaacggt
       61 atcgccgatg gtcaggactt catcgcgcgc cgggatcacc gcgacgacac ccacagcggt
      121 atcaggcggc cgcttggtgg gtaacaaggc aggtgagacc cgccagaatc cggcgcgtgc
      181 cagcaacaga tacaaccagg cgatcaacgg caacgcggcc attacgtgta cgatcacgca
      241 cggaatgata gtccaggagc ccattcgatg cgtcgcatct gccgtgtgct gtttctgctg
      301 atgccgttgt cgggcatgtc aagcgaactc acgatcgaac gtatctttgc cgatcccgct
      361 ctgtccgggc cgacgccccg ggcagtcaag gtttcaccgg acggtcgcag agtcggactg
      421 cttcggggac ggcctaccga tcagcatcag cttgatctgt ggacctacga cgtcaaagac
      481 gcctcgatgc agatgcgggt ggactcgaaa agacttgccc cgaccgagca actctccgat
      541 gcggagcgcg cgcgacgcga gcgcgagcgg atcgcagact ttcacgggat cgtgaactac
      601 gactgggcac ccgacagtca tcaggtgctc tttacactca acggcaatct atacctgtac
      661 gacctggacg cacccgccgc caccgctttg cgacaactgg cgcacggcgc gcaggcgatt
      721 gtggatcctc aggtgtcccc ccggggccgt tacgtatcgt tcgtgcgcga gcagaatcta
      781 tgggtcatcg atcttaacag cggcgccgag catcagctga cgacggatgg gggcggtgca
      841 gttcacaacg ccgaggcgga gttcatcgcg caggaggaga tgggacaaac cagcggctac
      901 tggtgggcac cggatgactc gatgatcgcc tacaaacgct tcgatgagag tggagtgccg
      961 atcgctcgac gcttcgaggt ctacgccgag cgtatcgacg tggtcgagca gcgctatccg
     1021 gccgcaggcg atccgaatgt cagcgtcaag ctcggcctcg tggcgccgac ggcttcggcg
     1081 acgcgctgga tcgatctggg ctccaatgcg gacatctacc tggtacgcgc cgactggaca
     1141 cccgatgcac gccagctcgc ctttcagcgt ctgtcgcgcg atcagaagcg gctggatctg
     1201 gtgctggtgg cggccgatac gctgaaagaa accgggctgg tcgcggaact ttcaaacacg
     1261 tggatcaacc tgtccgacga cctgcatttt ctaaagcgcc agcgggccct gatctggtct
     1321 tccgagcgca ccggtagcaa gcacctctac ctgatcgact tggacgggca cgtgcgatat
     1381 ccgctgaccg ccggcgcgtg gcacgtcgac gacctgttgg cagtggatga gcctgcgggg
     1441 ctgttgtact tcgccgcgaa caaggatgcc gtcatcgacg cacagattta cacggcaaga
     1501 ctcgacgggc gcgatccctc tcatcccaat cgcgtcagtc aaggcgacgg ctggcatgcc
     1561 gcgcagttct cgcgccaagc tccgcgcgtc gcgctgtatg ttgacacgta ctccgatccc
     1621 accaccccgc ctgaagtgag tatcaacgcc ccggacgggc atcgtctcgc ctggatcgaa
     1681 gccaatccgc tcgactcaaa tcacccctac tggccttatc acgagcgaca tgtcgcgccg
     1741 gagttcggcc atattcaggc tgaagacggc caggagttgg agtatgcgct gatgaagccg
     1801 ccgcgcttcg atccgtcacg gcgctatccg gtctttctcc cggtctacgg aggtccgaca
     1861 gtgcagcgtg tgcagcgtca ctggcctgat ctcatcaacg aatacatggc gcagcgcggt
     1921 tacatcgtgt tcgaactgga taatcgtggc tcctcacgcc gcaaccgcgc gtttcaggat
     1981 ccgattcatc tgcgcttggg agagatcgaa gcgcgcgatc agttggcggg cgcacgttgg
     2041 ctgcaaactc aggcatgggt ggaccccaaa cgcattggcg tcttcggctg gagctacggg
     2101 ggctatatga cgctgatgct cctcgccaag ggatcgaatg tcattgccgc cggggccgcc
     2161 gtcgcgcctg tcaccgattg gctgctgtac gacacctgct acaccgagcg ctatctggga
     2221 atgccgaagg acaactcgct cggctaccgg cagtcctcgg tattctcagc gctcgacgcg
     2281 atgacatcgc ccttgttcct ggcgcatggc atggctgacg acaacgtgct gttcgtcaac
     2341 tccacgcaac tgatgtcaga actgcaacgg cgcgaagttc aattcgagct gatgacctat
     2401 cccggtggca aacatgggct ctcgactccg cagatgaaaa cgcatgtcta ctccgcgatt
     2461 cagagatttt tcgacacgca tttgaaagct ggcagttgac atgcacccgc cgcgggcagc
     2521 tgcacatctg cggttggtac cgcgggcgca cccgcagcct tcaatcaatc aggcgagctg
     2581 accgttgcag ccgacgtcga ggcgtcagtg tgattggcag cggcgtgtcg ggcggccgca
     2641 cgcgccgctt cctccgcaga tgcaggcagt tcctgcgcga cgaggattgc ctgattcacc
     2701 accgacggat cgtcgcgcaa tctgacatac agttgaccgt cggtcggccg cggaaccggc
     2761 agcgtgtatc cgggaaatcc gtcaggcacc tgaaccggat gtccgaactg cgcatcgctc
     2821 gacaccgcgt ccaccaggaa caggttgctg cccgacaatt tgcaggccag ttccggcgtc
     2881 gcgggacact gcaattcgcg cagcgacggt aaacgaacca gcgtgaccag cggctgccag
     2941 tcgccggcgg taccgttggc gacgacgcga aactgcagtg ggccgaatgc ggatgggcca
     3001 aaggacttcg aggcatccag cgtcgccact gcgacgacgg aattct
//
LOCUS       DATMRM010000379         6065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2066610, whole genome shotgun sequence.
ACCESSION   DATMRM010000379 DATMRM010000000
VERSION     DATMRM010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2066610"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VNZ06_11445"
     CDS             complement(<1..183)
                     /locus_tag="VNZ06_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21412.1"
                     /translation="MPSRTPVMRWSALLAAAFGTCQAKPAGSAEPPAMVLHAARLLDV
                     ATGRMVTPGEVLVQGQR"
     gene            complement(173..727)
                     /locus_tag="VNZ06_11450"
     CDS             complement(173..727)
                     /locus_tag="VNZ06_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HWW21413.1"
                     /translation="MEKLFISADSLLRDSMELARLIVRSGLRPTYLVAMWRGGSPIGI
                     AVQEVLEYHAIEVDHIAIRTSSYLGIDQQNKTVRVHAVDYLVSRLSADDQLLLIDDVF
                     DSGRSLEAVIAELKRRCRRNLPDVIRIATVYYKPMRNRSTLIPDYYVHATDRWLVFPH
                     ELVGLTREEILQHKPVGEDFFNAV"
     gene            848..2008
                     /locus_tag="VNZ06_11455"
     CDS             848..2008
                     /locus_tag="VNZ06_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWW21414.1"
                     /translation="MESEFASLWGLDRSVLYLNHGSFGACPTAVLAAQTRLRTRMESE
                     PVDFLSATLPAGLHTARSALAHFLGANEQDLAFAPNATTAVNAVLQSLAFERDDELLL
                     TSHTYAACRKAVDWIAARSGVHVVVAQLPFPVRSEEEIVAAILTCVSPQTRLALLDHV
                     TSPTALVMPLGRLVRELQDRDVDCLVDGAHAPGMVPIRLNELGAAYYTGNAHKWLCAP
                     KGAAFLYARRDRQSALHPTVISHGYAAGFPEEFDWTGTFDPTPWLCIGESIHYLQGLL
                     PGGWPELMARNHALALRARTILLQALNIEAPCPEEMIGSMASIPLPTPSDGSPAARLD
                     FDGLHGWFRERGVETWLYPQPMLLVRLSAQLYNSVPQYERLATLLREAVYGR"
     gene            2332..2940
                     /locus_tag="VNZ06_11460"
     CDS             2332..2940
                     /locus_tag="VNZ06_11460"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWW21415.1"
                     /translation="MTAPAQRTCVTVLFVDLRGYTRLAERLPATRVVPLLDEFLNMLT
                     MTTERYGGQIYHVAGDGLMAGFGVHDPGQDGTLEAIAAGRAMLVRFEPIAVRWHREFS
                     IVTGIGIGIHRGEVARAYLGPPGNKTLTLIGDTVNVAARLCNRARAGEVLFSGSVAQS
                     DQIVARPIPEPKPFLRLPQFALKGRSAPLDIYCVPAPDRVAV"
     gene            3042..3749
                     /locus_tag="VNZ06_11465"
     CDS             3042..3749
                     /locus_tag="VNZ06_11465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HWW21416.1"
                     /translation="MSSIKALLFDVFGTLVDWRESIAREAARILIRQDIAVDWHRFAD
                     SWRDQYQPAMEEVRAGRLPFCKLDVLHRRNLDIVLKQFGLEHVDEAARTELNLAWHRL
                     DAWPDVASGLTRMRERFLLAPCSNGNVSLMVDLARRNGFPWDAILGAEIARDYKPKPI
                     VYRASAAAFDCDPEQTMMVAAHSSDLAAAARAGLRTAFVARPDERGPGRGESRASVLV
                     DLEVNDLNALADALLRD"
     gene            complement(3767..4333)
                     /locus_tag="VNZ06_11470"
     CDS             complement(3767..4333)
                     /locus_tag="VNZ06_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21417.1"
                     /translation="MLTTKLRFIPSVLFGLSMVGTAVATESESQSPQPAVWQTHHAQF
                     DYFGVTSRYTCDGLETKVRQILEYLGARPDLYVVATGCPRGPESFSRTAWVRVDFSTL
                     SAAAASGSPADAIAANWTPLKMNAQRPAFMGDGDCELVDHMRKLLTDNFSWRGKVAYR
                     TDCPIDTTEFHDYQIQGEVLKASGSAHR"
     gene            4534..5076
                     /locus_tag="VNZ06_11475"
     CDS             4534..5076
                     /locus_tag="VNZ06_11475"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWW21418.1"
                     /translation="MRLITLRALLAGGALLAACLSLQAQQPTAPNLDTVPDKMPFSEP
                     YGAPISAQRAQSLIQAAATEATKKGWPMNIAVVDAGANLVAFLRMDGAQLASIAIAEH
                     KARTAAKYRRPTKAFEDAIQKNDYKYILTLDDIVASRGGIPLVANGKVIGAVGCSGGT
                     GSQDEVVCQSAVAALAKSGQ"
     gene            complement(5070..5552)
                     /locus_tag="VNZ06_11480"
     CDS             complement(5070..5552)
                     /locus_tag="VNZ06_11480"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HWW21419.1"
                     /translation="MASVLFVCMGNICRSPMAEAVFRAYARHAAPELPIDVDSAGTHD
                     YHIGSAPDPRARRVAAGHGIDMSGLRARVLCLPDFERFEWIVAMDRQNMQAARRLAPS
                     ERRERVRLLLDYAPQQPLREVPDPYYGETADFELVLELTQQAAPGLLQFLRSELAKTY
                     "
     gene            complement(5561..>6065)
                     /locus_tag="VNZ06_11485"
     CDS             complement(5561..>6065)
                     /locus_tag="VNZ06_11485"
                     /inference="COORDINATES: protein motif:HMM:NF021571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2299 family protein"
                     /protein_id="HWW21420.1"
                     /translation="GGLMQQQQIESWLRQLGCTPNAVKSIEGNEWQMQFVFPPGSQQN
                     MACFSPRTPAGSTVISVNYPLGRGEAAYDAMSDLQKGAFLQELRLVLSRDYLYHDISP
                     GPTPTSSPRSFAVFDMRFDDGLTLDSFARSIIAVSNTALSANLCCARHLAGARPAPAA
                     TSAAQVQ"
BASE COUNT         1160 a         1876 c         1854 g         1175 t
ORIGIN      
        1 acgttgcccc tgaaccagca cttcgccggg tgtgaccatg cgccccgtgg caacgtcgag
       61 tagccgcgct gcgtgcagca ccatagcagg gggttctgcc gaacctgcgg gtttggcctg
      121 acaggttccg aacgcggccg ccagcagtgc gctccagcgc ataacaggcg ttctagacgg
      181 cattgaaaaa atcctccccc accggcttgt gctgcaatat ctcctcgcgc gtcaacccca
      241 cgagctcatg cgggaacacc agccagcggt cggtcgcatg cacgtaatag tcaggtatca
      301 gcgtgctgcg attgcgcatt ggcttgtagt agaccgtcgc gattcggatc acatcaggca
      361 gattgcgtcg acagcgccgc ttgagctccg cgatgacggc ctccagactt cgacccgaat
      421 caaatacgtc gtcgatcaac agcagctgat catccgccga caggcgcgac accaggtagt
      481 ccaccgcatg cacccgcacg gtcttgtttt gctggtcgat ccccaggtac gacgaagttc
      541 gaatcgcgat gtgatcgact tcgatcgcgt ggtactccag cacttcctgt acagcgatgc
      601 cgatggggct accgccgcgc cacatcgcaa ccagatacgt tggacgcagg ccgctgcgaa
      661 cgatcagtcg cgccaactcc atcgaatcac gcagcagcga atcggccgaa ataaacagtt
      721 tttccatcgc gtccgcactc ctgaaagtgc aattatgccc tctgcgtacg cgactggcgt
      781 tgtagggcga tatcaaccat cctagagacg accttgctgc cacgctcgct gcgaagagag
      841 gagcgacttg gagtcggagt ttgcgtcgct ttggggcctg gatcgctccg tgctgtattt
      901 gaatcacggc tcctttggtg cctgtcccac cgccgttctg gcggcccaaa cccgcctgag
      961 gacgcggatg gagagcgagc cggtggactt cctgtcggcg acgctgccgg ccggcctgca
     1021 caccgcacgc agtgcgctcg cacactttct cggcgcaaac gagcaagatc tggcgttcgc
     1081 tcccaatgcc accacggccg tgaacgccgt acttcaatcg ctggcattcg aacgggacga
     1141 cgagctgctg ctaaccagcc acacctacgc cgcctgtcgc aaagccgtcg attggatcgc
     1201 cgcacgcagc ggcgtgcacg tggtcgtggc acagctgcca tttccggtgc gcagtgagga
     1261 ggagatcgtc gcggccattc tgacctgcgt ctcaccacaa acgcggctcg ccttgctgga
     1321 tcatgtgacc agtccgactg cattggtgat gcccctcggc cgcctggtgc gcgaattgca
     1381 ggatcgcgat gtcgattgtc tggtcgatgg agctcatgca cccggcatgg tacctatccg
     1441 tttgaatgag ctcggcgctg cctactacac gggcaacgct cacaaatggc tatgcgctcc
     1501 gaagggcgct gcctttctct atgcacgccg cgaccggcaa tccgcgcttc acccgactgt
     1561 gatcagtcac ggttatgcgg cagggtttcc agaggagttt gactggaccg gaaccttcga
     1621 cccgacgcca tggctatgta tcggcgaatc aattcactac ctgcagggcc tattgccggg
     1681 aggttggccg gagctgatgg ctcgcaatca tgcgcttgcc ctgcgggctc ggacgattct
     1741 gttgcaagca ttgaacatcg aggcaccgtg tccggaagag atgatcggct cgatggcttc
     1801 cattcccctg cccaccccca gtgatggatc ccctgccgcg agactggatt tcgacggact
     1861 gcacgggtgg tttcgtgaac gcggagtcga gacctggctg tatccgcagc ccatgctgtt
     1921 ggtgcgtctc tcggcgcagc tctacaacag tgtgcctcaa tacgaacggc tcgcgacgtt
     1981 gttgagagaa gccgtgtatg gccgatgacg cagccgcgcg ccggcggtta ctgccgggtg
     2041 gtggcggacc gactggccgt tgttcagagc aggccatcgg ctgttcgatc gtccctggct
     2101 tggctttttc gagccgtaga ccacaggttt agtggcgtct gggcggcacc tgcggaccgt
     2161 tatgtccggg ttgcctcagc ggaatgggtc tggcacccga tctgacagaa aactggagtc
     2221 tgtccagatg agcgtgaccc atttatcgct tagaaatgtt caattccccc ttatgtaccg
     2281 gacgttggca ggatatgctc tcgctcgttg tgagcaacca tcgataagac catgaccgcg
     2341 ccggcacaga ggacctgtgt aaccgttctg tttgtagacc tccggggcta caccaggctg
     2401 gcggagcggt taccggcaac gcgggtcgtt ccgctgttgg acgaatttct caacatgctg
     2461 acgatgacca ccgaacgcta cggtgggcag atctaccacg tcgccggcga cggtctgatg
     2521 gccgggttcg gcgtccacga tcctggacag gatggaacgc tcgaggcgat cgcagcagga
     2581 cgcgcgatgc tcgtccggtt cgaacccatc gcggtccgat ggcaccgtga gttctccatc
     2641 gtcacgggaa tcgggatcgg cattcaccgc ggtgaagtcg cgcgcgcgta cctggggcct
     2701 cccggcaaca agacgctcac cttgatcggc gataccgtga acgtcgctgc gcgattatgc
     2761 aatcgcgccc gcgccggaga ggtgctgttc tccggttcgg tcgcgcagag cgaccaaatc
     2821 gttgcgcgac ccattccgga gccgaaacct tttctgcgat tgccgcagtt tgcactgaag
     2881 ggacgcagcg ctccgcttga catctattgc gtgcccgccc ccgatcgcgt tgcagtgtga
     2941 tcattttgtg atcaccccag gcaacgtggc atatttccgc atcgccggtt aagtcgccgc
     3001 gttttttgca cagacttatc caccggttct gtggataagt catgtcgtcc atcaaagctc
     3061 ttctgttcga tgtgttcggc accctggtcg actggcgcga atcgatcgcg cgcgaagccg
     3121 ccagaatatt gatccgacaa gacatcgcgg tcgattggca tcgctttgca gatagctggc
     3181 gcgatcaata tcaaccggcg atggaggaag tgcgcgctgg acgtctgccg ttctgcaagc
     3241 tcgatgtgct ccatcggcgc aatctcgaca tcgtgctcaa gcagtttgga ctggaacacg
     3301 tcgacgaggc cgcccggact gaactcaatc tcgcctggca tcgactggat gcctggcccg
     3361 acgtcgcgtc aggattgacg cgcatgcgcg agcgattcct gttggcgccc tgctcaaacg
     3421 gtaatgtctc cctcatggtg gatctggcgc ggcgcaatgg cttcccgtgg gacgccattc
     3481 tgggcgccga gatcgcccgc gactacaaac ccaaaccgat tgtctatcgg gcatcggctg
     3541 cagccttcga ttgcgatccg gagcagacga tgatggttgc ggcgcactcc tccgacctgg
     3601 cggccgccgc gcgcgctgga ctgcgcacgg cattcgtcgc gcgacctgac gagcgcgggc
     3661 ccggcagggg agaaagccgc gctagcgtgc tcgtcgatct cgaggtaaat gacctgaacg
     3721 ctctcgccga cgcgctgctg cgcgactgag ccgagggtga gtcgtttcaa cgatgcgctg
     3781 aaccgctggc cttcagtact tcaccttgga tctgatagtc gtggaactcc gtggtgtcga
     3841 tcgggcagtc ggtgcgatag gccaccttac cgcgccagct gaaattgtcg gtgagcagct
     3901 tgcgcatgtg atcgacgagt tcgcagtcgc catcccccat gaacgcgggt cgctgcgcat
     3961 tcattttgag cggtgtccag ttggccgcga tcgcatcggc cggactgccg ctggcagctg
     4021 cagcggacag cgtgctgaaa tccactctca cccatgccgt gcggctgaac gactcggggc
     4081 cccgcgggca gccggtcgcg acgacgtaca ggtcagggcg cgcgccgaga tattcgagaa
     4141 tctggcgtac tttggtctcg aggccgtcgc aggtgtagcg cgatgtgacg ccaaaataat
     4201 caaactgtgc gtgatgggtc tgccaaaccg ccggctgggg gctttgactc tccgactccg
     4261 tagcgacagc cgtacccacc atgctgaggc cgaacaatac tgacgggatg aatcgaagct
     4321 tagtggtcag cataggatga tgcctcgcgc gcagagcact ccatggaaga accccatact
     4381 agtccagcgc ggcacgttga gaaacgttga ccttgcgcac agcgttgccg cacggcagcg
     4441 agcttcggac ccgatccgat gcgctcgcac agcgcacgtg agccaatcgt gtagagtacg
     4501 acccgcatcg gaccacacag ggggcaaaca actatgcgtc tcattactct gcgcgcgctg
     4561 ctggccggcg gcgcgctgct ggcggcctgt ctatcccttc aagcgcagca acccacagcg
     4621 ccgaacctgg acacggtgcc ggacaagatg cctttcagcg aaccgtatgg tgctccgatc
     4681 agcgcccagc gcgcgcagtc gctgatacag gccgccgcga ccgaggcgac caagaagggc
     4741 tggccgatga acatcgccgt ggtggacgcc ggtgccaatc tggtcgcatt cctgcgcatg
     4801 gacggcgcac agctcgcttc cattgcgatc gccgaacaca aggcgcggac cgcggccaag
     4861 taccgtcgtc ctaccaaggc attcgaagac gcgattcaga agaacgacta caaatacatc
     4921 ctgacgctcg acgacatcgt cgcatcgcga ggcggaattc cgctggtcgc aaacggcaag
     4981 gtcatcggcg cggtgggttg ttcgggagga accggctcgc aggacgaggt ggtttgtcag
     5041 tctgccgtcg cggcactggc taagagcggt cagtaggttt tggccagctc gctgcgcagg
     5101 aactgcagca atcctggcgc ggcttgttga gtgagttcga gtacgagttc gaaatcggcc
     5161 gtctcgccgt agtaaggatc gggtacctcg cgcagcggct gttgcggggc atagtcgagc
     5221 agtaatcgta cacgctcgcg gcgctccgaa ggcgcgagac gccgcgcagc ctgcatgttc
     5281 tggcgatcca tcgccacgat ccattcaaag cgctcgaaat ccggaaggca caggacacga
     5341 gcgcgcaaac ccgacatgtc tatcccgtgg ccggcggcga cgcgccgcgc gcgtgggtcc
     5401 ggtgcgcttc caatgtgata gtcgtgggtc ccggccgaat cgacgtcgat cggcagctcc
     5461 ggagcagcgt gtcgtgcata ggcgcggaac accgcctccg ccatcggcga acgacagatg
     5521 ttccccatgc agacaaacaa gacgctggcc aagtacgccg ctactgcacc tgcgcggcag
     5581 acgttgcagc cggcgctgga cgcgctcctg ccagatgacg cgcgcagcac aggttcgcag
     5641 acagcgctgt gttgctgacg gcgatgatcg atcgggcgaa cgagtcgagt gtcaagccgt
     5701 catcaaaccg catgtcgaac accgcgaacg agcgcggcga ggacgtcgga gtgggaccgg
     5761 gagagatatc gtgataaaga tagtcacgac tgagcacaag tctcagctcc tgaagaaacg
     5821 cgcccttctg cagatcgctc atcgcatcgt aggccgcctc gccacgtccc aacggatagt
     5881 tgacggaaat gacggtcgat cccgcaggcg tacgcggaga gaagcaggcc atgttctgct
     5941 gacttccggg cgggaacacg aactgcatct gccactcgtt gccttcgatc gacttcaccg
     6001 cgttcggtgt acagccgagc tggcgtaacc aggattcgat ttgttgctgt tgcatcagac
     6061 ctccg
//
LOCUS       DATMRM010000380         8831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4252386, whole genome shotgun sequence.
ACCESSION   DATMRM010000380 DATMRM010000000
VERSION     DATMRM010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8831
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4252386"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..478)
                     /locus_tag="VNZ06_11490"
     CDS             complement(89..478)
                     /locus_tag="VNZ06_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWW21421.1"
                     /translation="MIDHSGVVVSDIARSKEFYRRALEPLGYRILAEFPAAMTGTVDV
                     AGFGVPPKPDFWISNGRPNVPPIHIAFRVDSRTLVEAFYRSALAAGGRDNGAPGPRPH
                     YHEHYFGAFVLDPDGHNIEAVCHDPVA"
     gene            complement(475..1710)
                     /locus_tag="VNZ06_11495"
     CDS             complement(475..1710)
                     /locus_tag="VNZ06_11495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW21422.1"
                     /translation="MADREVWTSTQRHVVTASFMGWMLDAFDFFLVVFVINQLSSEFH
                     APVKDVSEALFLTLAMRPVGAFLFGRIADHFGRKPALMIAVVFYSVAELATAASPSLT
                     AFLVLRALFGIGMGGVWGVGASLAFEAVPTSSRGFVSGLLQVGYPTGYLLASVVYGLL
                     FDHIGWRGMFILGAAPALLVFYIHRAVPESSVWTEMRNQPKASGLLHSLKGHWGLALY
                     AMILMACFNFFSHGTQDLYPTFLQKQHGFDTHMTSRIAITYNIGAIIGGLLFGTLSSR
                     MGRRKTIALASALALVAIPFWAYGTTAVGLAIGAFFMQVLVQGAWGVVPAHLNELAPA
                     GIRATFPGVVYQLGNLIASRNSVIQATLAEQHGSVAHPDYSYALALIAGVAAVLLVVL
                     ALIGPEKRDVHFGNPSPPP"
     gene            1822..3630
                     /locus_tag="VNZ06_11500"
     CDS             1822..3630
                     /locus_tag="VNZ06_11500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWW21423.1"
                     /translation="MLLRLDKVSLSFGARPLLDQVSLQIDRSERIALVGRNGEGKSSL
                     LRLILGEVEPEAGTLWLRPGARVAHLAQDITALSNETVAQIVTGGVPRAGAHDHEEWQ
                     VEQRVATLCSRLGLDPASRFDQLSGGWRRRALLARALIGQPEILLLDEPTNHLDVEAI
                     EWLEQFLLEYAGAALFVSHDRAFINHLATRIVELDRGALTATLGDYETYRRHKAHRLE
                     VEAAQQALFDKKLAQEEVWIRKGIEARRTRNEGRVRALYQLRRERRARRERTGQVQLQ
                     QHAAPESGSLVFDVAHLGLAYGDRTIIRDFSVRIMRGDRVGLVGPNGAGKSSLIKALL
                     GQIEVSQGEVRSGTRLEVAYYDQERAQLKLDESVMQNVAGRNDQVIVNGQSQHVSSYL
                     RDFLFRADQLNTPAGALSGGERNRLMLARLFSQPANLLVMDEPTNDLDIDTLELLEEY
                     VSEFHGTLLLVSHDRAFLDNVVTELLVFEGAGVVREFVGGYSDWAQFREQQKGSEPPR
                     RAVQTPQGVDRSPRQRRLSYKDQRELNSLPEQLERLEAQKQQLEAELSDTALYEGAPE
                     HVQERLGRLRALTQALEAGYARWGELEALSGQTLKP"
     gene            3646..4110
                     /locus_tag="VNZ06_11505"
     CDS             3646..4110
                     /locus_tag="VNZ06_11505"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HWW21424.1"
                     /translation="MKSRKTALAAVLLSAAVTLPLQAHHSFAEFDTTKTLTVICTVKE
                     FQWVNPHSWLIVVAPNEQGIAQEYRFEGVPPAVLTRSGWTDDTLLVGDRITLDYHPLK
                     DGTPGGAYVAVTLPNGKHLGTSGTSFVPFSPANTPPPAPRQPPPPTHPIIID"
     gene            4189..4920
                     /locus_tag="VNZ06_11510"
     CDS             4189..4920
                     /locus_tag="VNZ06_11510"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWW21425.1"
                     /translation="MKRTSTRRAILLAAASGAATLAPWARAVNQDDSQRNEDRGPGEA
                     KDVGSVEDLMREHGVLRRILLVYQQTAAKLRRGAAVDISALNHAAQLFRSFGEDYHEK
                     MLEESFIFPALRKVRGPAASYPEVLIAQHQRGRKITDYILAATNRRKLSGGHAVALAN
                     AFDGFALMYQNHAAREDTLVFPAWKQALAERDIDQIGDKFEDIEQQQFGKEGFETIVE
                     QVGAIEKSLGYEDLAQFTAPMPPTT"
     gene            4934..5413
                     /locus_tag="VNZ06_11515"
     CDS             4934..5413
                     /locus_tag="VNZ06_11515"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF003806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HWW21426.1"
                     /translation="MTRTIYLLNGPNLDLLGEREPQIYGRFTLADIEALCRTVTDRHG
                     LTLTAHQTNAEHEMIQWLHDAREAAGIVINPAAFCYHSVPILDALKMCAGPIVEVHIS
                     NIYRREDWRSKSILATAASGVITGLGIDGYRLAMEYLVGKLPAPKTASRTSAKNKRS"
     gene            complement(5416..6615)
                     /locus_tag="VNZ06_11520"
     CDS             complement(5416..6615)
                     /locus_tag="VNZ06_11520"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HWW21427.1"
                     /translation="MREPILIINAGSSSLKFSVFETLENGALAPKLHGQVETVGSESH
                     LRVADASGKSLADERIKRGGDEAIRALQHWFAAHTGTQKGFAGVGHRIVHGGTTYSQP
                     VLIDGSVMAGLQQLVPLAPLHEPQQIAAIRAIRATSPHLKQVACFDTAFHSNQPPIAR
                     MFALPREYTARGIRRYGFHGLSYEYIVAALPALAPECVGARLIVAHLGNGASLCAIHQ
                     GQSIATTMGFTALDGLVMGTRCGSLDPGVLLYLLQHEHLGAAEIERLLYERSGLLGVS
                     GVSSDMRELLASDRPEAAEAVELFVYRISRELGSLASALGGVDALIFTGGIGEHAAPI
                     RARVCQQAAWLGVTLDASANDRGERRICDAGSRVSAWVIPTDENLMVARHTRRLLDAA
                     AVRTSAT"
     gene            6771..7052
                     /locus_tag="VNZ06_11525"
     CDS             6771..7052
                     /locus_tag="VNZ06_11525"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HWW21428.1"
                     /translation="MPTQPTPTSRSPRRSTAKTAATKSTGTTGSRAADATAPAKGIDP
                     EDRRALIEQAAYFRAENRNFAPGFEVQDWLSAEAEVDTLLALGVPTTDH"
     gene            complement(7061..7390)
                     /locus_tag="VNZ06_11530"
     CDS             complement(7061..7390)
                     /locus_tag="VNZ06_11530"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HWW21429.1"
                     /translation="MRIELTEVLWFEEHSITLAELSELCALPLPLLQELVGGGAIEPL
                     ETAGSEPRFGAQALNAARAARRLRDDFDLDASGLLLALGLLERVQELEREIRTLRARL
                     PGPLNPR"
     gene            complement(7380..8333)
                     /locus_tag="VNZ06_11535"
     CDS             complement(7380..8333)
                     /locus_tag="VNZ06_11535"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HWW21430.1"
                     /translation="MKYKDYYAILGVERAASEADIKKAYRRLAHKYHPDVSKEAGAEE
                     RFKEVAEAYQTLKDSDKRAAYDQLGSHSAGQDFQPPPDWRRQWQPSEEAGFSVDELDL
                     SALFEHLRSGRGAGGGRRSMAGQDYEVAVPITIEQAYSGAEIDLDLEMPEYDAAGRVH
                     RTPHAFKARIPKGATDGQRLRIPGKGGKGINGGRNGDLYLNVSLRPHPYYRAAGHDLY
                     LDLPLAPWEAVLGTSVEVPTPGGSVMLKVPAGTQAARQLRLPGRGLPKPKSGQGDLYA
                     VVQIVVPGTVSERERALYRELSESSAFDPRAHFVAEGSHAN"
     gene            8565..>8831
                     /locus_tag="VNZ06_11540"
     CDS             8565..>8831
                     /locus_tag="VNZ06_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21431.1"
                     /translation="MYRTLSLVGAALLSTVLSACGSDSTTATPSASHAHGTLAISPPF
                     RIASVDAATFQSQLSANSGGPQLLQIAGTPVCGVDFYYINFWTQG"
BASE COUNT         1743 a         2851 c         2733 g         1504 t
ORIGIN      
        1 gggtaggaac ttagagaaaa tttgccgtca cactgcacta attgccatga attaatttca
       61 cagcgaccat gcaatgcggc aacgcgtttt acgccacggg gtcgtggcag accgcttcga
      121 tgttgtgccc gtcgggatcg agaacgaacg cgccgaaata gtgctcgtga taatgcggcc
      181 gcgggcccgg ggcgccgttg tcacggccac cggccgccaa cgcgctccgg tagaacgcct
      241 ccaccagcgt ccgactgtca acccgaaacg cgatgtgaat cgggggcacg ttcgggcggc
      301 cattcgaaat ccagaaatca ggcttgggcg gaacgccaaa gccggccacg tccacggtac
      361 cagtcatcgc cgcggggaac tcggcaagta tgcgatagcc gagcggctcc agcgcgcggc
      421 gatagaactc tttgctgcgc gcgatatcac tgacgacgac accgctgtga tcgatcatgg
      481 tggggggctc gggttgccga agtgcacgtc gcgcttttcg ggtccgatga gcgccagcac
      541 caccagcagc accgcggcga cccccgcgat cagggccagc gcgtacgagt agtcaggatg
      601 cgcgacgctg ccatgttgtt cggccagcgt ggcctgaatg acggaattcc tggacgcaat
      661 cagattgccg agctggtaca ccacacctgg aaatgtggcg cgaatgcctg ccggcgccag
      721 ctcattcaga tgggcaggca ccacgcccca ggcgccctgc accagcacct gcatgaagaa
      781 cgcgccgatc gcgagcccga ctgcggtggt cccataggcc cagaacggaa tggccaccag
      841 cgccagggct gaggccagcg cgatggtctt gcgccgaccc atgcggctcg aaagcgtgcc
      901 gaacagcaga ccgccgatga tcgccccgat gttgtaagtg atcgcgatac gcgaggtcat
      961 gtgcgtatcg aatccgtgtt gcttctgcag gaacgtcgga tacagatcct gcgtcccgtg
     1021 gctgaagaag ttgaagcacg ccatcaatat catggcgtag agcgcaagcc cccaatgtcc
     1081 tttgagcgaa tgcagcaagc cgctcgcctt gggctggttg cgcatctcgg tccagacgct
     1141 cgactccgga accgcgcgat gaatatagaa caccagcagc gctggcgccg cgcccagaat
     1201 gaacatgccg cgccagccga tgtgatcgaa cagcaatcca tagaccaccg aggccagcag
     1261 atacccggtg ggataaccca cctgtaacag tcctgacacg aagccgcgcg agctggtcgg
     1321 aaccgcctcg aaggcgagcg atgcgccgac accccacacc ccgcccatac cgatcccaaa
     1381 caaggcacgc agcaccagga acgccgtcaa cgacggcgaa gcagcggtgg caagttcggc
     1441 gaccgagtaa aacaccacgg cgatcatcaa tgcgggtttg cgtccaaagt gatcggcgat
     1501 gcgtccgaac aggaaagcgc ccaccggacg catcgcgagc gtcaggaaca acgcttcgga
     1561 aacatccttc accggcgcgt ggaattccga ggacagctga ttgatcacga atacgaccag
     1621 gaagaaatcg aacgcgtcga gcatccatcc catgaaactc gcggtgacga cgtgacgctg
     1681 ggtggaagtc cagacttcgc gatcagccat gatccccgtc cctgttgtcg ttgtaacggc
     1741 acacccatca tagcgccgcg cacacgaacc gtcagtgtga ccgttagaat gtaactttct
     1801 tttacttggt acggagtccg attgttattg cgcctcgaca aggtgtcgct cagttttggc
     1861 gctcggccgc tgcttgacca ggtatcgctg cagatcgacc ggagcgagcg catcgcgctg
     1921 gtcggacgaa acggcgaggg caagtcctcg ctcctgcgcc tcattctggg agaggtggaa
     1981 ccggaggcgg gcaccctctg gctgcggccg ggtgcgcggg tagcccattt ggcgcaggac
     2041 atcaccgccc tctcgaacga gacggtcgca cagatcgtca ccggcggcgt cccccgtgcg
     2101 ggcgcgcacg atcacgagga gtggcaagtc gagcagcgtg tcgcgacgct gtgttcacgc
     2161 ctggggttgg acccggcatc gcgattcgac cagttgtccg gcggctggcg gcggcgcgct
     2221 ctgttggctc gcgccctgat agggcagccg gagatcctgc tgctcgatga gcccacgaat
     2281 catctggatg tcgaagccat cgaatggctg gagcagttcc tgctcgagta cgccggcgct
     2341 gcgctgttcg tcagtcatga ccgcgccttc atcaatcacc tggctacccg catcgtcgaa
     2401 ctcgatcgcg gcgcattgac cgcgacactc ggcgactacg aaacctatcg tcgccacaag
     2461 gcccaccggc tcgaggtgga ggccgcccag caggcgctgt tcgacaagaa gctggcacaa
     2521 gaggaggttt ggatccgcaa aggcatcgag gcgcgtcgta cccgcaacga aggccgcgta
     2581 cgcgcgctat atcaactgcg ccgtgagcga cgcgcacggc gcgaacgcac gggtcaggtt
     2641 caattgcagc aacacgcggc gccggagtcg ggaagtctcg tgttcgacgt cgcacacctc
     2701 gggctggcgt acggggaccg gacgatcatc cgggatttct cggttcgcat catgcgcggc
     2761 gatcgtgtcg gtctggtcgg tcccaatggc gccggcaaga gctcgctgat caaagcgcta
     2821 ctgggacaga tcgaagtgtc acagggcgag gtgcgcagcg gcacgcggct ggaagtcgcg
     2881 tactatgatc aggaacgcgc acagctcaag ctcgacgaat cagtcatgca gaacgtggcc
     2941 gggcgcaacg atcaggtgat cgtcaatggt cagtctcagc acgtttccag ctatctgcgt
     3001 gacttcctgt ttcgtgctga ccagctcaat accccggccg gcgcgctgtc cggcggtgag
     3061 cgcaatcgcc tgatgctggc acggctgttt tcccagccag ccaatctgtt ggtgatggat
     3121 gagcccacga acgacctgga catcgatacg ctggagttgc tcgaggaata tgtctccgag
     3181 ttccacggga cgctgctgct ggtgagccat gatcgcgcgt ttctggacaa cgtcgtcacg
     3241 gagctgctgg tgtttgaggg cgccggcgta gtacgtgaat tcgttggcgg ctacagcgat
     3301 tgggcgcagt ttcgcgagca gcagaagggc tcagagccgc cgcgtcgcgc cgtgcagaca
     3361 ccgcaggggg tggatcgaag ccccaggcag cgccggcttt cctacaagga tcagcgcgaa
     3421 ctgaactcgc tgccggagca actggaacga ctcgaggctc agaagcagca gctcgaggcg
     3481 gagttgtccg atacagctct atatgagggc gcacccgaac acgtgcagga acgcctgggc
     3541 cgactgcggg cactgacgca ggcactggaa gccggctacg cgcgctgggg cgagctcgag
     3601 gccttgagtg gccagactct caagccctga gagataatgc ggtggatgaa gtcgcgcaag
     3661 accgcgctgg ccgctgtgct gttgtcggca gcagtgacgc tgcctctgca ggcgcaccac
     3721 tcctttgcgg agttcgacac cacgaaaacc ctgaccgtga tctgcacggt caaggagttc
     3781 cagtgggtca atccgcacag ctggctgatc gtcgtggcgc ccaacgagca agggatcgcg
     3841 caggaatacc gctttgaggg cgtgcccccg gccgtcctga cgcgcagcgg ctggaccgac
     3901 gacaccctgc tggtaggaga cagaatcacg ctggactatc acccgctgaa ggacggcacg
     3961 cccggaggcg cctatgtcgc ggtcacactg cccaatggca agcacctggg aaccagcggt
     4021 acctcgttcg tgccgttttc gccggccaac acaccacctc cagcaccgcg gcagccacct
     4081 ccgcccactc atcctataat cattgactag taccgctggc ggccgcggcg tcgcgacgcg
     4141 agccgcgctt cccctacaat gctcgagggt gaacggagaa accggggcat gaagagaacc
     4201 tcgacacggc gtgcgatcct gctggctgcg gcatcaggcg ccgccaccct cgccccgtgg
     4261 gcgagagcgg tcaatcagga tgacagtcag cgcaacgagg atcgcggtcc cggggaggcc
     4321 aaggacgtcg gttcggttga ggatctgatg cgcgagcacg gcgtgctgcg ccggatcctg
     4381 ctggtgtacc agcagacggc agccaagctg cgccgcggag ctgccgtgga tatcagtgcc
     4441 ctgaaccacg ccgcgcagct gtttcggtct ttcggcgagg actatcacga aaagatgctc
     4501 gaggaatcct tcattttccc ggcgctgcgt aaggtgcggg gacccgcggc ctcctatcca
     4561 gaggtgctga ttgcgcagca tcagcgcggc cgcaagatca ccgactacat tctcgctgct
     4621 acgaaccgtc gcaagctctc cggcggccac gccgtggcgc tggcgaatgc cttcgatggc
     4681 ttcgcactca tgtaccagaa ccacgcggcg cgtgaagaca cgctggtgtt tccagcatgg
     4741 aagcaggcac tggccgaacg cgacatcgat cagatcggtg acaagttcga agacatcgaa
     4801 cagcagcagt tcggcaaaga aggctttgag acgatcgtcg agcaggtcgg cgcgatcgaa
     4861 aaatccctcg gctacgaaga tctggctcag ttcacagcgc ccatgcctcc gacgacctaa
     4921 ggtagcaccg acgatgacgc gcacgattta cctgctcaat ggaccgaacc tcgacctgct
     4981 cggagagcgc gagccgcaga tctatggccg cttcacgctg gcggacatcg aggcactgtg
     5041 tcgcacggtg acggaccggc acggtctgac tttgacggcg catcaaacca acgccgaaca
     5101 cgaaatgatc cagtggctgc acgacgcgcg cgaagccgca ggaatcgtca tcaacccggc
     5161 cgcgttctgc tatcactcgg ttccgattct ggatgccctg aagatgtgtg ccggaccgat
     5221 tgtcgaagtg cacatcagca acatctatcg ccgcgaagac tggcgcagca aatccattct
     5281 cgcgaccgcg gccagcggcg tgatcacagg gcttggcatc gacggctacc gcctggcgat
     5341 ggaatacctg gtcggcaaac tgcccgcgcc gaagacagcg tcccgcacgt ctgcgaagaa
     5401 caagcgcagc tagcttcagg tggcgctcgt ccggacagcg gcagcatcca gcagccggcg
     5461 cgtatgccgg gcaaccatca gattctcatc ggtgggaatc acccacgccg acacgcggct
     5521 gcccgcatcg caaatgcgcc gctctccgcg gtcattggct gatgcgtcca gcgtcacgcc
     5581 cagccaggcc gcttgctgac acacgcgcgc gcgaatcggg gccgcgtgtt cgccgatgcc
     5641 accggtgaaa atcagggcgt ccacgccgcc gagcgccgac gccagtgaac ccagttcgcg
     5701 gctgatgcga tagacgaaca gttccaccgc ctcggcggcc tccggccgat cactcgccag
     5761 cagctcgcgc atatcggacg agacgcccga gacgcccagc agccctgagc gttcgtacag
     5821 cagccgctcg atctccgcgg ccccgagatg ctcatgctgc agcaggtaga gcagtacgcc
     5881 cggatcgagc gacccgcaac gcgtgcccat caccagacca tccagcgcgg tgaatcccat
     5941 cgtcgttgcg atgctttgac cctgatgaat cgcacacagg ctcgcgccgt tccccaggtg
     6001 cgccacgatc aggcgtgctc cgacgcactc cggggcgagc gcgggcaagg ccgcgacgat
     6061 gtattcgtag gacagcccgt ggaaaccata gcgccggatg ccgcgcgccg tgtattcacg
     6121 cggcagggca aacatccgcg cgatcggcgg ctggttggaa tgaaaggccg tatcaaaaca
     6181 ggccacctgc ttcaaatgcg gcgaggtcgc gcggatagcg cggatcgcgg cgatctgctg
     6241 cggttcatgc agcggtgcca gcggcacgag ttgctgcagc cccgccatca cgctgccgtc
     6301 gatcagcact ggctgcgaat aggtggtgcc accgtgcacg atgcgatgtc ccactcccgc
     6361 gaaccccttt tgggtaccgg tatgggccgc aaaccaatgc tgcaacgccc gaatggcctc
     6421 atcaccacct cgcttgatgc gttcgtccgc gagcgatttg ccgctggcat ccgccacgcg
     6481 cagatgcgac tcactgccga cggtttccac ctgtccgtga agtttcgggg cgagcgcacc
     6541 attctccagg gtctcgaaaa cggaaaactt caggctcgat gagccggcat tgatgatcag
     6601 gattggctca cgcatcgcag cgaaccctcg acggcgaata gcgtgcgcat gatagcgacg
     6661 tttgtgtacc gacaggcgcg actgtcggca aaaaggaacg ttggtctttt cagatgaata
     6721 gagcgaagat aatcagcgct tgaagtgcac ttctaaatgg aggttcttct atgccgacac
     6781 agcctacgcc gacgtcgcgt agtccgcgtc gctcaaccgc caagacggcg gccactaaga
     6841 gcacggggac tacaggttcg cgggcggcgg atgcgactgc accggccaag ggcatcgatc
     6901 ctgaggatcg acgcgccctg atcgagcaag ccgcttactt tcgcgccgag aatcgcaact
     6961 ttgcgcctgg ttttgaagtt caggactggc tgagcgcaga ggcggaagtc gataccttgc
     7021 tagcgttggg agtaccaacg accgaccact gaccgatgcg ctaacgcggg ttcaatggac
     7081 ccggcaggcg ggcacgcaac gtgcgaatct cccgctcgag ttcctgtacg cgctcaagca
     7141 gtccgagcgc cagcagcaac cccgaggcat ccagatcgaa atcatcgcgc agtcgccgcg
     7201 ctgcgcgcgc cgcgttcaat gcctgcgcgc cgaagcgagg ctcggatccc gccgtctcca
     7261 ggggttcgat cgccccgcct ccgaccagtt cctgcagcaa tggcagcggc agagcgcaca
     7321 actccgacag ttcggccagc gtgattgagt gctcctcgaa ccacagcacc tccgtcagct
     7381 caattcgcat gactgccctc cgcgacgaaa tgcgcgcgcg gatcaaaggc agaactctcc
     7441 gacagctccc gatagagcgc gcgctcgcgc tcggaaaccg taccaggcac cacgatctgc
     7501 accaccgcgt ataggtcacc ctgtccactc ttcggcttcg gcaggccgcg tcccggcagg
     7561 cgcaactgcc gcgcggcctg cgtcccggcc ggtactttca acatgacgga gccaccgggc
     7621 gtgggtacct cgacgctcgt gccgagtacc gcttcccacg gggccagtgg caggtccagg
     7681 taaaggtcgt gaccggcagc gcggtagtac ggatgcggcc gcaaactgac gttcaggtac
     7741 agatcgccat tgcgcccgcc gttgataccc ttgccgccct tgcccgggat gcgcaaacgc
     7801 tgcccatcgg ttgcaccttt ggggattcgt gccttgaagg cgtggggtgt ccgatgcacg
     7861 cgtcccgccg catcgtactc tggcatctcc agatccagat cgatttccgc gcccgagtaa
     7921 gcctgctcga tcgtgatcgg gaccgcgacc tcgtagtcct gcccggccat cgacctgcgc
     7981 cccccgcctg ctccgcgacc ggagcgcaga tgctcaaaca gcgccgacag atccagttcg
     8041 tcgacgctga agcctgcctc ctcagagggc tgccattgcc gccgccagtc cggcgggggc
     8101 tgaaagtcct gccctgccga gtgcgagccg agctggtcat aggccgcgcg cttgtcggag
     8161 tccttcaggg tctgataggc ctcggcgact tccttgaagc gctcttcggc gccggcctcc
     8221 ttcgaaacgt ccggatggta cttgtgcgcc aggcggcgat aggccttctt gatgtcggcc
     8281 tcgctcgctg cacgctcaac gccgaggatc gcgtagtagt ccttgtattt catgagtgtt
     8341 gccgccgttc aggatcataa cctagcacct gccggctcag agttcgcctc cccagcatca
     8401 ccgacccatc gcatgctgct ttgagtgggg acggcgcttg ccgttctcaa ccggccgatt
     8461 cgagcggccg gccgtcggtg cattcccacc ggccctcaag cggatgccga ctggcggccg
     8521 cgtgtagaat cgcgccgcgc cgcagcggta ttcgggagga tcgaatgtat cgaacactga
     8581 gtctggtggg cgcggcgttg ctgagcactg tgctgagcgc atgcggatcg gatagcacca
     8641 ccgccacgcc ttccgcgtcc cacgctcacg ggacgctagc catcagtccg ccgtttcgga
     8701 ttgcttcggt cgacgccgcg accttccagt cgcagctttc agccaacagt ggcggcccac
     8761 aattgctgca gatcgccggc acgccggtct gcggggtaga tttctactac atcaatttct
     8821 ggacccaggg c
//
LOCUS       DATMRM010000381         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_198753, whole genome shotgun sequence.
ACCESSION   DATMRM010000381 DATMRM010000000
VERSION     DATMRM010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198753"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VNZ06_11545"
     CDS             complement(<1..530)
                     /locus_tag="VNZ06_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013844528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HWW21432.1"
                     /translation="MPWTKITREQYRRDELRYASDMTDAEWRLIERLLPRAHRRGRPR
                     RTDLRSVVEAVLYILSTGCQWRALPQEFPPYSTVQGYFYAWRDTGRWQRIVSVLVRRA
                     RKKLGRAPRPTAAVIDSQSAPTTQAGGPRGYDAGKRIYGRKRHIVTDTNGLLLGVHVH
                     PANVQDCHGAVPLLKKV"
     gene            complement(694..1038)
                     /locus_tag="VNZ06_11550"
     CDS             complement(694..1038)
                     /locus_tag="VNZ06_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HWW21433.1"
                     /translation="MIRQSGEAPQIIEYLKNPPDRARLIELISAMGIPVRALLREKGT
                     PYAELGLADPKWSDDQLIDFMLAHPILINRPIVQTPKGTKLCRPSEAVLDLLDHPVGC
                     FVKEDGEVVTRT"
     gene            complement(1092..>2030)
                     /gene="arsB"
                     /locus_tag="VNZ06_11555"
     CDS             complement(1092..>2030)
                     /gene="arsB"
                     /locus_tag="VNZ06_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HWW21434.1"
                     /translation="VNLPVAGLVWLMIIPMLLKIDFGALGQVREHWRGVGVTLFINWA
                     VKPFSMALLGSLFIGHLFAPWLPSTQISSYIAGLILLAAAPCTAMVFVWSNLCEGEPH
                     YTLSQVALNDVIMIFAFAPLVGLLLGVASITVPWATLLISVMLYIVVPVIVAQLWRRA
                     LMRSGPAALERTLHVLQPVSLVALLTTLVMLFGFQGEQIIAQPLVIVLLAVPILIQVY
                     LNAGLAYWLSRRFGVAWCVAAPAALIGASNFFELAVAAAISLFGLNSGAALATVVGVL
                     VEVPVMLSVVYLVKASRRWYEAGAEPSVAVNKEART"
BASE COUNT          427 a          674 c          578 g          351 t
ORIGIN      
        1 accttcttca gcagcggtac cgcgccatgg cagtcctgga cgttggccgg atgaacatga
       61 accccgagaa gcaggccatt agtatcggtc acaatatgcc gcttgcggcc gtagatgcgc
      121 tttcccgcgt catagccgcg cggcccgcca gcctgcgtag ttggtgcact ttggctgtcg
      181 atcacggcgg ctgtcggtct tggcgcgcgt cccagctttt ttcgtgcccg ccggacaagg
      241 acactgacga tcctttgcca ccgtcccgta tctcgccacg cataaaaata gccctgcact
      301 gtcgaataag gcgggaactc ctgcggcaac gcccgccact ggcagcccgt cgacagaata
      361 tagagaaccg cctccacaac actacgcaga tcggtcctgc gcggccgacc gcgccggtgt
      421 gctcgcggca aaaggcgctc gatcagcctc cactccgcat cggtcatatc gcttgcataa
      481 cgcagttcgt cccgccgata ttgctcccga gtgattttag tccagggcat cgtgatctcc
      541 ttcgaatctt cgcaaatccg atggaatcac acctcactga gatcactcac ccatttttca
      601 gtcagactct gaggagcgcg ctcttgcgcg cgtctcgaag gatgagcgac atggttcgag
      661 acgccgcttt gcggctcctc accatgaggc cttctacgtc cgcgtgacga cctcgccgtc
      721 ctctttgaca aagcacccga cgggatgatc gagcaggtcg agcaccgctt ccgacggtcg
      781 gcagagtttc gtgcccttgg gcgtttgcac gatcggtcgg ttgatcagga tcggatgcgc
      841 cagcatgaaa tcgatcaact ggtcgtcgct ccatttcgga tcggcaagcc caagctccgc
      901 atagggagtg cccttttccc gcaacagcgc gcgaaccggg atacccatcg cgctgatcag
      961 ttcgatcagc cgcgcccgat ccggcgggtt cttcagatat tcgatgatct ggggcgcctc
     1021 gccgctttgc cggatcattt ccagcgtgtt gcgcgacgtg ccgcaagccg gattgtgata
     1081 gatcgtgacg ctcacgtccg cgcttccttg ttgacggcaa cggaaggctc agcaccggcc
     1141 tcataccagc ggcgcgacgc cttcaccagg tagaccaccg agagcatcac cggcacctcg
     1201 accagaactc ccaccacggt ggccagcgcc gccccggagt tgagaccgaa caggctgatg
     1261 gccgcggcga ccgcaagttc gaagaagttg ctggcgccaa tcagcgccgc cggcgcggca
     1321 acgcaccagg ccacgccgaa gcgccggctc agccagtagg caagccctgc gttgagatag
     1381 acctggatca ggatcggcac cgcgagcagc acgatcacca gcggctgcgc gatgatctgt
     1441 tcgccctgaa agccgaacag catcaccaac gtggtcagca gcgcgaccag cgataccggc
     1501 tgcagcacat gcagcgtccg ctctagcgcc gccggcccgg acctcatcag cgcacgccgc
     1561 catagctgcg caacaatgac gggcaccacg atatacaaca tcaccgaaat cagcagggtc
     1621 gcccagggca cggtgatcga ggcgacgccg agcaagagac cgaccagcgg cgcgaacgca
     1681 aatatcatga taacgtcgtt aagcgcgacc tggctcaagg tgtagtgcgg ctcaccttcg
     1741 cacaggttag accatacgaa caccatggcc gtgcaaggcg ctgccgccag cagaataagc
     1801 ccggcgatat aggacgaaat ttgtgtcgat ggcagccatg gcgcgaagag atggccgatg
     1861 aacagcgagc ccagcaacgc catcgaaaac ggtttcaccg cccagttgat gaacagcgtc
     1921 acgccgacgc cgcgccagtg ctcccggacc tggccgagcg cgccgaaatc gatcttcagc
     1981 agcatcggaa tgatcatcag ccagaccagc ccggccaccg gcagattgac
//
LOCUS       DATMRM010000382         2271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1799386, whole genome shotgun sequence.
ACCESSION   DATMRM010000382 DATMRM010000000
VERSION     DATMRM010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799386"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VNZ06_11560"
     CDS             complement(<1..768)
                     /locus_tag="VNZ06_11560"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HWW21435.1"
                     /translation="MSGNITTLTGGISLRASSLIALATVSALALSSCGDGFCHSCLAY
                     LPPGPASQLSFGLVAGNFANNALPSIVETSTLDNGYSPNPGNLLTYLSLTTGSYPTSP
                     TQTPDGNDPVYLGAADLNGDHLVDLVSANSQDGTLAVFFNSASSPGTFSTPLVLDSPG
                     ASQLAIADMNGDGLPDLISADFNVSLFLQSSPGAFANPISLYSGGANWVAVGDLNHDG
                     SPDVALTDNTGVKIVFHTGAAGATTYAAATQVYTESAN"
     gene            complement(896..1390)
                     /locus_tag="VNZ06_11565"
     CDS             complement(896..1390)
                     /locus_tag="VNZ06_11565"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWW21436.1"
                     /translation="MIAQRARVSIRVDLPPALAFEIFTGEIDRWWRRGPRFRHAGQRG
                     GFIRIEPQVGGRLFESIDSPHGQSVFEVGRVRVWQPPSRVAFSWRNANFAPEESTDVE
                     VEFSATRSGTLVTVTHQGWEALPEEHPARHGTHGADFVRMIGMWWGQQMSSWRELTIE
                     YRKP"
     gene            complement(1394..1738)
                     /locus_tag="VNZ06_11570"
     CDS             complement(1394..1738)
                     /locus_tag="VNZ06_11570"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWW21437.1"
                     /translation="MGPDLLDATFAALADPTRRAVIRALLQKRRRAGELAILVKMSPP
                     ALSRHLRVLRKAGLIVEQYREDDARVRVFTVDRRALGPVRDWLEQVDEMWQTQLEAFK
                     AHAERRGSSRGS"
     gene            complement(1748..2203)
                     /locus_tag="VNZ06_11575"
     CDS             complement(1748..2203)
                     /locus_tag="VNZ06_11575"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWW21438.1"
                     /translation="MKAAPADWPRISSALCYQDAARAIDWLCDAFGFEVRLKVEGQDG
                     RIEHSELTYGEGLIMVAQEDTQGTRHLKTVLRSPKSLGGKGTQSLMFFVDDVDAHCAH
                     ARARGARIIEEPTLQDYGTEYWADRSYGALDPEGHVWWIVQRVRSAPRQ"
BASE COUNT          457 a          707 c          699 g          408 t
ORIGIN      
        1 attcgcactc tcggtgtaga cctgagtggc ggcggcatag gtcgtggcgc cggcagcgcc
       61 ggtgtggaac acaattttga caccggtgtt gtccgtcagt gcgacatccg gtgatccgtc
      121 gtggttcaga tcgcccaccg cgacccagtt ggcgccaccc gaatacaggc tgatcgggtt
      181 ggcgaacgcg cccggtgagc tctgcaggaa cagcgagaca ttgaagtcgg cggagatcag
      241 atcgggcaac ccgtcgccgt tcatgtcagc gatcgcgagc tgcgaggcgc cgggggaatc
      301 gagtacgagt ggggtgctga aggtgccggg actgctcgca ctattgaaga acacggccag
      361 cgtgccgtcc tgggagttcg ccgataccag atccacgaga tgatcgccat tcagatcggc
      421 cgcgcccagg tagaccggat cattgccatc gggcgtctgt gtcggagagg tcggatagga
      481 ccctgtggtc aatgacagat acgtcagcag gttgcccggg ttcggtgaat acccgttgtc
      541 gagggtgctg gtctcgacga tcgatggcag tgcgttgttg gcaaagttgc cggcgacgag
      601 gccaaatgaa agctgactgg caggccctgg cggcaggtag gcaaggcagc tgtggcagaa
      661 gccatcgccg cagctggaaa gggcaagcgc ggaaacggtg gcaagggcga tgagggacga
      721 ggcgcgcaga ctgattccgc cagtaagcgt cgtgatgttg ccagacatgg gtgcctccca
      781 ttcgatccaa accggtacaa cgacaacggc ctaaagccta cgaggttgac gttgccaatg
      841 ccgcctaatc tatcgaaggg aatcgctcac agggtggcaa aaattaccag caaattcacg
      901 gttttcgata ctcgatggtc aattcccgcc atgaactcat ctgctgcccc caccacatgc
      961 cgatcatgcg gacaaaatcg gcgccgtggg tgccatgcct ggccggatgc tcctcaggca
     1021 gcgcctccca gccctggtgt gtcacggtca cgagcgtgcc ggagcgcgtg gccgagaatt
     1081 caacctcgac gtcggtggac tcttccggcg cgaagtttgc gttgcgccag gagaacgcca
     1141 cacggctggg cggctgccag actcgcacgc gtccgacttc gaatacgctc tgaccgtgcg
     1201 gcgagtcgat cgactcgaac aggcgcccgc ccacctgcgg ctcgatgcga atgaatccgc
     1261 cgcgctgtcc ggcgtgtcga aatctcggcc cccgacgcca ccatcggtcg atctcgccgg
     1321 tgaagatttc gaacgccagc gccggtggta ggtcgacgcg gatgctgacg cgggcacgct
     1381 gcgcgatcat gctctaagat ccgcgcgacg agcctcgccg ttcggcgtgc gctttgaagg
     1441 cctcaagctg agtctgccac atctcgtcca cctgctcgag ccagtcgcga accggaccca
     1501 gggcgcgccg atcgaccgta aagacacgca cgcgtgcatc gtcctcgcgg tactgctcga
     1561 cgatgagacc ggccttgcgc agcacccgca gatgacgact cagcgccgga ggactcatct
     1621 tgaccagaat ggccagctcg cccgcccgcc gacgcttctg cagcagcgcc cgaatcacag
     1681 cgcggcgtgt cggatcggcc agtgccgcaa aggttgcgtc caacagatca gggcccacgt
     1741 cgtgaactca ctgtctcggt gcactgcgaa cgcgctgcac gatccaccag acgtgaccct
     1801 caggatcgag tgcaccgtag ctgcgatccg cccagtactc ggtgccgtag tcctgcagcg
     1861 tcggctcctc gatgatgcgt gcgccgcgtg cgcgcgcatg agcgcagtgc gcatcgacat
     1921 catcgacgaa gaacatcaac gattgcgttc ccttgccgcc aagcgatttc ggactgcgca
     1981 gcacggtctt cagatgacgc gtaccctggg tatcctcctg cgccaccatg atcagacctt
     2041 cgccataggt cagctcgcta tgctcgatgc ggccgtcctg cccttcgacc ttgaggcgca
     2101 cttcgaaacc aaatgcatcg cacagccaat cgatggcgcg ggcagcgtcc tggtagcaca
     2161 gcgcgctcga aatgcggggc caatcggccg gagcagcttt catacgcacc tccaatcttc
     2221 catatagtta aatgcatagt taaatattaa cgacatcgtt aacaatatgc a
//
LOCUS       DATMRM010000383         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3300617, whole genome shotgun sequence.
ACCESSION   DATMRM010000383 DATMRM010000000
VERSION     DATMRM010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3300617"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1069
                     /locus_tag="VNZ06_11580"
     CDS             56..1069
                     /locus_tag="VNZ06_11580"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006850.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HWW21439.1"
                     /translation="MEAFIRGLPKAELHLHLEGTLEPALALRLARKHRIELPHSTIAE
                     LQHSYQFTDLQSFLDIYLAASKVLRDEDDFHALTSAYLRRAHSQGIVHVEVFFDPQSH
                     TERDIAFVTVLGGIRRALLQGEREFGISHRLILCFLRHLSAAEAMRTLEQALPHKAMI
                     AAVGLDSTESGHPPAKFADVFERARGEGLLAVAHAGEEGPASYVRDALDLLKVQRIDH
                     GVRCEEDPQLVDRLLRERIPLTMCPLSNVRLKVFPRVADHNIRRLLQRGLCVTINSDD
                     PAYFGGYLNENYLAVQQQLGATPDELVQLARNSFEASFLPTPQKSRWLRAIDDYVRRT
                     PVQ"
     gene            1120..1446
                     /locus_tag="VNZ06_11585"
     CDS             1120..1446
                     /locus_tag="VNZ06_11585"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWW21440.1"
                     /translation="MTYETALQCLSDSTRRRVFERLRAGPQSVGALAKGMPVSRPAVS
                     QHLKALKEAGLVSDRAEGARRVYYIDPNGLGPLRRWIDEFRDDALQSFKRQVEAANPT
                     AGRKRA"
     gene            1523..1942
                     /locus_tag="VNZ06_11590"
     CDS             1523..1942
                     /locus_tag="VNZ06_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011214128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWW21441.1"
                     /translation="MKYLHTMVRVTDLAASCRFYCDALGLHEVRRKEVEKGRFTLVFL
                     AAPGDEEAQVELTYNWDPENYSGGRNFGHLAYEVEDIYATCRRLIERGITINRPPRDG
                     HMAFVRSPDQISIELLQKGGALTPAEPWVSMPNVGVW"
     gene            complement(1950..2105)
                     /locus_tag="VNZ06_11595"
     CDS             complement(1950..2105)
                     /locus_tag="VNZ06_11595"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HWW21442.1"
                     /translation="MEKYDVATVGHSGSGGEYPTQDGFGWTNGVMRKFSALYPADLAR
                     TAVEQCR"
BASE COUNT          397 a          682 c          657 g          384 t
ORIGIN      
        1 ggattggaga tgcaattgcc cgcagccggc gcagttgcgg tacgtataat gcagcatgga
       61 agcgttcatc cgcggcctgc ccaaggcgga actgcacctg catctggaag gcactctgga
      121 acccgcgctg gcgctgcggc ttgcccgcaa acaccgtatc gagctgcctc attccaccat
      181 tgcagaactg cagcattcct atcagttcac cgatctgcag tcgttcctgg acatctacct
      241 ggcggcatcc aaagttctgc gcgatgaaga tgacttccac gcactgacct cggcctatct
      301 gcggcgcgcg cacagccagg ggatcgtgca cgtcgaggtg tttttcgatc ctcaaagcca
      361 cactgagcgc gacatcgcat tcgtcacggt gctcggcggc attcgccgcg ctctgttgca
      421 aggggaacgg gagttcggaa tctctcaccg cctgattctg tgtttcctgc gccaccttag
      481 cgccgccgag gcgatgcgca cgctggagca agcgctgccg cacaaggcga tgatcgcggc
      541 cgtgggactg gattcgaccg agagcggcca tccaccggcc aagttcgccg atgtattcga
      601 acgcgcacga ggcgagggat tgctcgcggt cgcgcacgct ggcgaggagg gtccggcgag
      661 ctacgtgcgc gacgcgctcg acctgctcaa ggtgcagcgt atcgaccacg gcgtgcgctg
      721 cgaagaggat cctcagctgg tggaccgtct gctgcgcgag cgcatcccgc tgacgatgtg
      781 cccgctgtcc aacgtcagac tcaaggtgtt cccgcgcgtt gccgatcaca acatccgccg
      841 gctgttgcag cggggactgt gcgtcacgat caactcggat gaccccgcct acttcggcgg
      901 ctatctgaac gagaactatc tggcggtcca gcagcaactg ggtgccaccc ccgacgaact
      961 ggtgcagctg gcgcgcaatt cgttcgaagc ctccttcctt ccaacgccgc agaagagccg
     1021 ctggctgcgc gcgatcgacg actacgtgcg gcgcacaccg gtccagtgat ctcatccgcg
     1081 ctgctttgac attcggtaag tttataatta ccgtaagcca tgacttacga gacagccttg
     1141 cagtgcctga gtgactccac gcgccggcgg gtattcgagc ggctgcgcgc gggaccgcaa
     1201 tccgtcggag cgctcgcaaa gggaatgccg gtcagccgtc cggccgtctc ccagcatctg
     1261 aaggcgctga aggaggcggg acttgtgagc gatcgggccg aaggcgcgcg gcgcgtctac
     1321 tacatcgatc ccaatggcct gggtcccctg cgccgctgga tcgacgagtt ccgggacgac
     1381 gccctgcaat cattcaaacg ccaagtcgag gcagcgaacc cgaccgccgg gaggaagcga
     1441 gcgtgatgac gaacccgcct gagcggcgct gcacgcggat gtggcgctgc gcgccggccg
     1501 cagcccgtgg cagaatgcgg gcatgaaata cttgcacacg atggtccgtg tgaccgacct
     1561 cgccgcctcc tgcagattct actgtgatgc actcggactg catgaagtcc ggcgcaagga
     1621 agtcgaaaaa ggacgcttca cgctggtatt tctcgccgca cccggcgacg aagaagcgca
     1681 ggtcgaactg acctacaact gggatccgga gaattactcg ggcggccgca acttcggcca
     1741 tctcgcctac gaggtcgaag acatctacgc gacctgccgc cgactgatcg agcgcggtat
     1801 cacgatcaac cgccctccgc gcgatggtca catggcgttc gttcgatcgc cggatcagat
     1861 ttcgatcgag ctgctgcaaa agggtggcgc gctgacgccg gcagagccct gggtatcgat
     1921 gccgaatgtc ggggtgtggt agtcgcgcac taacggcact gctcgaccgc agtgcgggca
     1981 agatcggccg gatagagcgc gctgaacttg cgcatcacgc cgttggtcca tccgaatccg
     2041 tcctgcgtcg gatactctcc accgctgcca ctgtggccga cggtcgcgac gtcgtacttt
     2101 tccaccagct tgccgctctc
//
LOCUS       DATMRM010000384         7525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_552003, whole genome shotgun sequence.
ACCESSION   DATMRM010000384 DATMRM010000000
VERSION     DATMRM010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7525
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552003"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VNZ06_11600"
     CDS             complement(<1..210)
                     /locus_tag="VNZ06_11600"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWW21443.1"
                     /translation="MTSREDAASSAFQNAGSHAKPRVRDRIFDTACELFYRHGIRAVG
                     VDAIASEAGTNKMSFYRNFASKDELV"
     gene            385..1635
                     /locus_tag="VNZ06_11605"
     CDS             385..1635
                     /locus_tag="VNZ06_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWW21444.1"
                     /translation="MSLRSISSKASSIALLGIAAAVLSACGHSQAAVPAASLTLPQVT
                     VAPVLVQPLRHWHDLTGTLQAVDTVEVHPRVSGYINSVHFVEGAQVAKGELLFQIDPR
                     PFQFEVERLRAEVERARSHLELTRADHARAERLISQNAIAREEYEQLSSAETEATAAL
                     AATQAQFDAARLNLEFTQVRSPINGRASRALITPGNLVSNASALTTVVSDDPIYAYFD
                     ADEATYLTFTQRAAAHSGHDASSRSAPVYMGLVGEDGYPHQGRLDFLDNQIDSRSGTI
                     RARAVFDNRDGHFTPGLFARIKLVSADSYQAVLVDDRAIGTDLDKKYVLVLQPDHSVA
                     YRPVQLGPDIDGLRVVQSGLAAGDVIVVNGLQHVTPGAKVQPSVARMQGNREALGQVA
                     ASDPVETPPARPDGSMLAVARRAR"
     gene            1632..4814
                     /locus_tag="VNZ06_11610"
     CDS             1632..4814
                     /locus_tag="VNZ06_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HWW21445.1"
                     /translation="MNISRFFIERPIFAAMLSGLILLGGLIALPGLPISEYPEVVPPT
                     VVVNAQYPGANPSTIAETVASPLEQSITGVEDMLYQYSQATSDGRMRLTVTFKLGADV
                     DKAQVQVQNRVAQALPKLPQEVQRLGVTTNKSSPDITLAVFVYSPDARYDQTYLANYV
                     ALHIQDELSRLDGVGDVQRFGGGDYSMRVWLDPDAVAARNMTAGDVVAAIREQNVQVA
                     AGQLGAPPVGDAANFQLAINTKGRLVDEQDFSNIILKTGTDGQIVRLRDVARVELGSD
                     NYVLRSMLNNRTAVGIPIFQRPGSNAIKVSDEVRAKMAELSKSFPAGIDYNIAYDPTV
                     FVRGSINAVAHTLLEAIGLVVIVVLLFLQTWRASIIPLLAVPVSLIGTFAFMRIFGFG
                     LNALSLFGLVLAIGIVVDDAIVVVENVERNIGNGLSPLDATRQAMREVTGPIIATALV
                     LCAVFVPTAFISGLSGEFYKQFALTIAISTVISAFNSLSLSPALSAVLLRAHDAPPDR
                     LTSVLNAAFGWLFRPFNRLFTRASHAYVGGVARVMRVSAIALFVYGGLMALTYVQFAR
                     TPTGFVPSQDKQYLVAYAQLPDAASLGRTEGVIRRMTDIALGTPGVESVPAFPGLSIN
                     DFTNATNAGIAFTPLNSFESRRSPEQSAGAIAAKLNAQYGAIQEAQIAVFPPPPVQGL
                     GTIGGFKLQIEDRGNLGPTELYRQTQNLIAKARTRPELAGLFSSFQVSVPQVQVDVDR
                     DKAKVADVALQDLYDTMQVYLGALYVNDFNRFGRTYQVNAQAQPGFRLTAEDIGRLKT
                     RNQRGDMIPLSSFVTVHESAGPDRVMHYNTFLSADINGAPAPGYSIGQAQKAIEEVAR
                     ADLPRGMTFEWTELTYQQILAGNTMVYVFPLVVLLVFIVLAALYESLSLPLVVILIVP
                     MVLFSAILGVQLSGGDNNIFTQIGLIVLVGLACKNAILIVEFAKDREAAGESIWQAVL
                     DACRLRLRPILMTSVAFIMGVVPLVLSRGAGAEMRHAMGVAVFSGMLGVTAFGLLLTP
                     VFYLTIRKLTARRARAVKALALHPAPGGAI"
     gene            4811..6211
                     /locus_tag="VNZ06_11615"
     CDS             4811..6211
                     /locus_tag="VNZ06_11615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWW21446.1"
                     /translation="MNRRLPAIALWSGFVLMLMAGCAVGPNYQPPAQAAMVAHNLDAA
                     HFSATAPASNWWTQFQDPELSSLERRALQADPDVHTAIARVSAARASFHEAALDFAPH
                     LPINASYSQSKEQYPGLSTERIDIRSYAAGFDASWELDLFGHTRREAQAASAQLQFQQ
                     ATLQDTQVTVAAEVARNYFELRGVQHELAVTRENLDNEREALHLTQIRYDAGRVTELD
                     VDSAQARLKATEAELPQREAQQKAYGYRLAVLLGVRPGDLDSELERQTPSPARDTPLP
                     IGDVSNLLRHRPDVRAAERALAASSAQVGVATAELFPRVTFDGVIGFLTGDASQFGKA
                     ASRAWSYTPTVSWTGLDFPTAQARLRAAKADSEVALAAYQKAVLTALEDFENACVSYR
                     TQQTRLASVLEQEQASRRAADLAQIQYREGRINFLVLLDAQRTLLQADEDVAAAQTAV
                     NTSAVAVYKALGGIGT"
     gene            complement(6222..>7525)
                     /locus_tag="VNZ06_11620"
     CDS             complement(6222..>7525)
                     /locus_tag="VNZ06_11620"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HWW21447.1"
                     /translation="LVSVTELEKLAPQYPWNAALSAAGISRTTPRGERSLIISEKSAF
                     PELAAIFAQTPVAVWRDYLTLHYLHAHADYLSKQIDDVDFGFYGTVLGGQQQQLSRDL
                     RGIHLLDELMGEALGKLYCAQYFPPEAKVKSQRLVANLLKAYEEDIKTLQWMTPATRQ
                     KALEKIHTFNPKIGYPDKWRDYSALSIRRDDLIGDVRNANVFEHERQIHRIDLPVDKA
                     EWFMSPPTNNAYYNPTLNEIVFPAGILQPPYFDPNADDAVNYGEIGATIGHEISHGFD
                     DQGSKFDASGRLHNWWTPTDRKDFDARTTLLAKQYDSYEPLPGLHLNGRLTLGENIAD
                     LAGLVIAHKAYQIALGGKPAPVLNGYSGDQRFYIAYGQSWREIWSDGLTRRIVLSNPH
                     SPSKFRVDGVVRNDDGWYAAFPQIKAGDTYYLAPQQRVRLW"
BASE COUNT         1316 a         2417 c         2396 g         1396 t
ORIGIN      
        1 gacgagttcg tccttggagg cgaaattccg atagaaactc atcttgttgg tgccggcctc
       61 gctggcgatc gcgtccacgc ccaccgcccg aatgccatgc cgatagaaca gctcgcatgc
      121 ggtgtcgaaa atgcgatcgc gcacgcgcgg cttggcgtgg ctgccggcgt tctggaacgc
      181 gctcgaggcg gcgtcttccc tgctcgtcat atcgtcatgg ctcctcggcg atcgtggcca
      241 gtattgacat tcgacgtgac cgatcggtaa catcgcgccc agatgtgact gaacggtaac
      301 tcacggcgcc tagaatgaac cttcgacgcg cctcggtcaa gcggttcagc tcattgagta
      361 tcaacgaatt ttccgggagc ccctatgtcc ttgcgaagca tttcctccaa agcctcctcg
      421 atcgcactgc tcggtatcgc tgccgccgtg ctcagtgcct gtggccacag tcaggcggcc
      481 gtgcccgccg ccagcctcac actgccgcag gtcacggtcg cgccggtgct ggtgcaaccg
      541 ctgcgtcact ggcatgacct caccggcacg ctgcaggcgg tggacacggt ggaagttcat
      601 ccacgggtca gcggttacat caacagcgtg cactttgtgg aaggtgcgca ggtcgcgaaa
      661 ggcgagctgc tgtttcagat cgatccgcgt ccgtttcagt tcgaggtcga gcgcctgcgc
      721 gccgaagtcg agcgcgcacg ttcgcacctt gagctgacgc gcgccgatca cgcccgcgcc
      781 gaacggctga tatcgcagaa tgcgattgct cgtgaggaat acgagcagct cagctccgcc
      841 gagaccgaag ccaccgcggc gctggcggcc acccaggcac agttcgatgc cgcgcgcctc
      901 aacctggaat tcacccaggt gcgctcgccg atcaacgggc gcgcctcgcg ggcactcatc
      961 acgccgggaa acctggtctc caacgccagc gcgctgacca cggtcgtcag tgatgatccg
     1021 atctacgcct acttcgacgc ggatgaggcg acctatctga ccttcacaca gcgcgcagcg
     1081 gcccattccg gccatgacgc ctcctcgcgc tcagctccgg tctacatggg actggtgggt
     1141 gaggacggct atccgcatca gggtcggctc gatttcctgg acaaccagat cgactcccgc
     1201 agcggtacga tccgggcgcg cgcagtgttt gacaaccggg atggccactt cacgccggga
     1261 ctgtttgccc gcatcaagct ggtgtcggcc gacagctatc aggcggtgct ggtggatgat
     1321 cgcgcgatcg gcaccgatct cgacaagaag tacgtgctgg tgctgcagcc ggatcattcg
     1381 gtggcctatc gtccggtgca gctcggtccg gatatcgacg gactgcgggt cgtgcagagc
     1441 ggactggcgg ccggtgacgt aatcgtcgtc aacggactgc agcacgtcac acccggcgcc
     1501 aaggtgcagc cgagcgtcgc gcgcatgcag ggcaaccggg aggctttggg ccaggtcgcc
     1561 gcgagcgatc cggtcgaaac accgcccgcg cgtcccgacg gctcgatgct cgccgttgcc
     1621 cggagagcaa gatgaacatc tcgcgcttct tcatcgagcg gccgatcttc gccgcgatgc
     1681 tctcgggcct gatcctgctg ggcgggctga tcgccctgcc cggcctgccg atcagcgagt
     1741 atccggaagt cgtccccccg accgtggtgg tgaacgcgca gtaccccggt gccaatccga
     1801 gcacgattgc cgagacggtg gcctcgccgc tggaacaatc gatcactggc gtcgaggaca
     1861 tgctctatca gtactcgcag gcgacctcgg atggccgcat gaggctgacg gtgacgttca
     1921 agctcggcgc cgacgtcgac aaggcgcagg tgcaggtgca gaaccgggtc gcgcaggcgc
     1981 tgccgaagct gccgcaggaa gtgcagcggc tgggcgtcac caccaataag tcctcaccgg
     2041 atatcacgct cgcggtgttc gtgtattcgc ccgacgctcg ctatgaccag acctatctgg
     2101 cgaactacgt ggcgcttcac atccaggatg agctgtcacg actcgacggt gtcggggacg
     2161 tccagcgctt tggcggcggc gactactcga tgcgggtatg gctcgatccg gatgccgtgg
     2221 ccgcgcgcaa catgactgcg ggcgatgtgg tggcggcgat tcgcgaacag aacgtccagg
     2281 tcgcggccgg ccagctcggt gcgcccccgg tcggggacgc ggccaacttt cagctcgcga
     2341 tcaacaccaa gggacgactg gtcgatgagc aggatttctc caacatcatt ctcaagaccg
     2401 gcacagacgg gcagatcgtg cggctgcgcg acgtcgcgcg ggtggaactg ggctcggaca
     2461 actatgtgct gcgttcgatg ctcaacaacc gcacggcggt cggcatcccg atcttccagc
     2521 gcccgggcag caacgccatc aaggtatccg atgaagtgcg cgccaagatg gccgaactca
     2581 gcaagagttt tcccgcgggc atcgactaca acattgccta cgatccgacc gtattcgtgc
     2641 gcgggtcgat caacgcggtc gcgcatacgc tgctcgaggc gatcgggctg gtcgtgatcg
     2701 tcgtgctgct gttcctgcag acctggcgcg cctcgatcat cccgctgctg gccgtaccgg
     2761 tgtcgctgat cggcaccttc gccttcatgc gcatcttcgg cttcggactc aatgcgttgt
     2821 cgctgttcgg cctggtgctg gcgatcggca ttgtggtcga tgatgcgatc gttgtagtcg
     2881 agaacgtgga gcgcaacatc ggcaatggcc tctcgccgct cgatgcgacg cgccaggcga
     2941 tgcgcgaagt caccggtcca atcatcgcca cagccctggt gctgtgcgcg gtgttcgtgc
     3001 cgaccgcgtt catcagcggc ctgtcgggcg agttctacaa acagttcgcc ctgacgatcg
     3061 ccatcagtac ggtgatctcg gccttcaact cgctcagcct gtctccggcg ctgtcggcgg
     3121 tgctgttgcg tgcgcacgat gcgccgcccg atcgcctgac gagcgtgctc aacgctgcct
     3181 tcggttggct gttccggcca ttcaaccgcc tattcacgcg cgccagtcac gcctatgttg
     3241 gaggcgtcgc gcgcgtgatg cgggtgagcg cgattgcact gttcgtctac ggcggactga
     3301 tggcgctgac ctatgtgcag ttcgcacgca cgcccaccgg ctttgtcccc tcacaggaca
     3361 agcaatacct ggtggcctac gcgcagctgc cggatgcggc cagtctcggg cgcaccgagg
     3421 gcgtgatccg acggatgacc gacattgcgc tgggcacgcc gggtgtcgaa tcggtcccgg
     3481 catttccggg tctgtcgatc aacgatttca ccaacgcgac caacgcgggc atcgccttca
     3541 caccgttgaa ttcttttgaa agccgtcgct cgcctgagca gtcagccggc gccatcgccg
     3601 ccaagctcaa tgcgcagtac ggcgcgattc aggaggctca gatcgcggtg tttccgccac
     3661 cgccggtgca gggcctgggc acgatcggcg gcttcaagct gcagatcgag gaccgcggca
     3721 atctcggtcc cacggaactg tatcgccaga cacagaacct gatcgccaaa gcgcgcacgc
     3781 ggcctgagct ggccggcctg ttctcgagct tccaggtgag cgtgccgcag gtacaggtcg
     3841 atgtcgaccg cgacaaggcg aaggtcgccg acgtcgcgct gcaggatctg tatgacacca
     3901 tgcaggtcta cctgggcgcg ctctacgtca acgacttcaa tcgattcggg cgtacctatc
     3961 aggtgaatgc gcaggcgcag ccgggcttca ggctcacggc cgaggacatc ggccgcctca
     4021 agacccgcaa ccaacgtggc gacatgattc ccttgagcag tttcgtcacc gtgcatgaga
     4081 gtgccggtcc ggaccgggtc atgcactaca acaccttcct gtccgctgat atcaacggcg
     4141 cacccgcccc gggctacagc atcggccagg cgcagaaggc gatcgaggaa gtcgcgcgcg
     4201 ccgatctgcc gcgtggcatg actttcgagt ggactgaact cacctatcag cagattctgg
     4261 ccggcaacac catggtctac gtgtttccgc tggtggtgct gctggtgttc atcgtgctgg
     4321 cggcgctcta cgagtcgctg agcctgccgc tggtggtgat cctgatcgtg ccgatggtgc
     4381 tgttcagcgc aatcctggga gtgcagctct ccggtggcga caacaacatc ttcacgcaga
     4441 ttggtctcat cgtgctggtc ggtctggcct gcaagaatgc gatcctgatc gtcgagttcg
     4501 caaaggaccg cgaagccgct ggcgagagca tctggcaggc ggtgctcgat gcctgccggc
     4561 tgcgtctgcg gccgattctc atgacctcgg tcgcattcat catgggagtg gtgcccctgg
     4621 tgctatcgcg cggtgcgggc gccgagatgc gccatgcgat gggcgtcgcc gtgttctccg
     4681 gcatgctcgg cgtcacggca ttcggactgc tactgacccc ggtgttctat ctgaccatca
     4741 ggaagctcac cgcccgcaga gcgcgcgccg tgaaggccct tgcgctacac cctgcaccgg
     4801 gaggtgcaat atgaatcgcc ggctgcctgc gatcgcgtta tggagtgggt tcgttctcat
     4861 gctgatggcc ggatgcgcgg tcggcccgaa ctatcagccc ccggcgcagg cggcgatggt
     4921 ggcacacaac ctggatgccg cgcacttctc ggcgaccgcg ccagcgagca attggtggac
     4981 gcagtttcag gaccccgagc tgtccagcct cgagcggcgc gcgctgcagg ccgatcccga
     5041 cgtgcacacg gcgatcgcgc gagtgagtgc cgcacgcgcc tcattccatg aggccgcact
     5101 ggacttcgct ccgcacctgc caatcaacgc ctcctacagc cagtcgaagg agcaataccc
     5161 gggactgagc accgagcgta tcgacatcag gagctatgcg gccggattcg atgcgagctg
     5221 ggaactggat ctgttcggcc acacgcgccg cgaagcgcag gccgctagcg cgcagctgca
     5281 gttccagcaa gccacgctgc aggatacgca ggtgacggtc gcggccgaag tggcccgcaa
     5341 ctacttcgag ctgcgcggcg tacagcacga actggccgtg acgcgcgaga atctcgataa
     5401 cgagcgcgaa gcgctgcatc tgacgcagat tcgttatgac gcgggtcgcg tcacggaact
     5461 ggatgtagac agcgcacagg cgcgtctgaa ggctaccgag gccgagttgc cgcaacggga
     5521 ggcgcagcag aaggcctacg gctatcgcct ggccgtgctc ctgggcgtgc gacccgggga
     5581 tctggattca gaactggaga gacagacccc ttcgccagcg cgcgacaccc cgctcccgat
     5641 cggggatgtc tccaacctgc tgcgtcaccg gcccgacgta cgtgcggcgg aacgcgctct
     5701 ggcggcctcc agcgcacagg tgggcgtggc cacagcggaa ttgttcccgc gcgtgacctt
     5761 tgacggtgtc atcggctttc tgacgggcga tgccagccag ttcggcaagg cggccagccg
     5821 cgcctggtcg tataccccga cggtgagctg gaccgggctg gattttccga ccgcgcaggc
     5881 ccgtctgcgc gcggccaagg ccgacagcga ggtcgcactg gcggcgtatc agaaggcggt
     5941 actcacggcg ctcgaggatt tcgagaatgc gtgcgtcagc taccgcacgc agcagacgcg
     6001 gctggcgagc gtgctcgagc aggaacaggc ctcccgccga gccgctgacc ttgcgcaaat
     6061 acagtaccgc gagggccgca tcaatttcct ggtgctgctg gatgcgcagc gaacgctgct
     6121 gcaggcggac gaggacgtgg ccgccgccca gaccgccgtc aataccagtg cggtggcggt
     6181 ctacaaagca ctgggtggca tcggcacgtg acgagcaccg cctaccacag gcgcacgcgc
     6241 tgctgcggtg ccaggtagta cgtatcgccg gctttgatct gcgggaaagc ggcgtaccag
     6301 ccgtcgtcgt tgcgcaccac accgtccacc cggaacttcg agggactgtg cgggttcgac
     6361 aggacgatcc tgcgcgtcaa gccgtcgctc cagatctcgc gccagctctg cccgtacgcg
     6421 atatagaagc gctgatcgcc gctgtagccg ttgagcaccg gcgccggctt gccgccgagc
     6481 gcgatctgat aggccttgtg agcaatcacc agcccggcga gatcggcgat gttttccccg
     6541 agggtcagtc ggccattgag atgcagaccc ggcagtggct cgtaactgtc gtattgcttc
     6601 gcgagtagtg tggtgcgcgc atcgaaatcc ttgcggtctg tcggggtcca ccaattgtgc
     6661 agcctgcccg aagcgtcaaa cttgctgccc tgatcgtcaa agccatgact gatctcgtga
     6721 ccgatcgtcg cgccaatttc gccgtagttg accgcatcat ccgcattcgg atcgaagtac
     6781 ggaggctgca gaatgcccgc cgggaacaca atctcgttca gcgtcgggtt gtaataggcg
     6841 ttgttggtcg gcggactcat gaaccactcg gccttgtcca ccggcagatc gatgcggtga
     6901 atctggcgct catgctcgaa cacgttggca ttgcgtacat cgccgatcag atcatcccgc
     6961 cggatcgaga gcgcgctgta gtcacgccac ttgtccggat agccgatctt cggattgaag
     7021 gtatggatct tctcgagcgc tttctgtcgc gtggccggcg tcatccactg cagcgtcttg
     7081 atgtcttcct cgtacgcctt gagcagattg gccaccagcc gctggctttt gactttggct
     7141 tccggcggaa agtactgcgc gcaatacagc tttccaagcg cctcgcccat cagttcatcc
     7201 agcagatgga tgccgcgcaa atcgcgtgac agctgctgct gctgaccgcc gagcaccgtc
     7261 ccatagaaac cgaaatcgac gtcgtcgatc tgcttcgaca gatagtcggc gtgcgcgtgc
     7321 aggtagtgca gcgtcaggta atcgcgccag acggccacgg gggtctgcgc gaagatggcg
     7381 gccaactctg gaaacgccga tttctcgctg atgatgagac tgcgctcgcc gcgcggggtg
     7441 gttcgtgaaa tgccggcggc gctgagcgcc gcattccagg gatactgcgg cgccagcttc
     7501 tccagctcgg tgacgctcac caggt
//
LOCUS       DATMRM010000385         4302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4257462, whole genome shotgun sequence.
ACCESSION   DATMRM010000385 DATMRM010000000
VERSION     DATMRM010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4257462"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2047)
                     /gene="smc"
                     /locus_tag="VNZ06_11625"
     CDS             complement(<1..2047)
                     /gene="smc"
                     /locus_tag="VNZ06_11625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HWW21448.1"
                     /translation="MRLNKVRLAGFKSFVDPTSVHLPGNLTGVVGPNGCGKSNIIDAV
                     RWVMGELSARHLRGESMADVVFNGSSARKPVGNASVELVFDNSDGKIGGAYAAYSEIS
                     LKRAVSRDGSSAYFINGGRCRRKDITQLFLGTGLGSRSYAIIEQGMISRVIEARADDM
                     RAFVEEAAGISLYKERRRETESRMVDTRENLARLQDLRDEVDKQIRHLQRQANVARRY
                     QELKAQERTLTAELLALRLRELDSGAAVQDSAVRECELTMQQALADQRSAEAAIERQR
                     EFHTELSDTASRVQGRFYELGAEVTRAEENIRYTRELRERARNDLAQVNVSLTSIAQQ
                     IAHDEQQLAALRAEIATLEPQVEARRGSEQSSAGALADAEAELATWQQNWENFNRELG
                     AASQSEQVESARIEQLESQWHRLRAQADRLELEQDSLTSQHADSPIDGLSEREAAARS
                     AADALGRELESALNQVQQLRASQQSTDARVERLRRERDQARSELMSLEALQKAALREK
                     NPRIGAWLAALGRTPRPRLAETIDVQSGWERAVETVLGDYLEAVGVDTLEQLESSLPG
                     LDTGHLTFFEHLTAGAKATAGTLAEQVRGPNAALNVLERVHTAPSLAAALQRRSQLPN
                     GESLITAAGEWVGRDWLRVNRGGDVHAGVLEREHRLKGLRELCGAADARVVQLEQEAV
                     "
     gene            2139..2528
                     /gene="queF"
                     /locus_tag="VNZ06_11630"
     CDS             2139..2528
                     /gene="queF"
                     /locus_tag="VNZ06_11630"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HWW21449.1"
                     /translation="MPTQPSTEIKTFPNPTPERDYTIRMRIPEFTCLCPMTGQPDFAT
                     LELEYVPDRACVELLALKLYIWSFRNRGAFHEAVTNEILSHLADAVSPRFMRLSAVFN
                     VRGGIYTTVVAERRAADWRPAEPVRLP"
     gene            2670..3464
                     /gene="dksA"
                     /locus_tag="VNZ06_11635"
     CDS             2670..3464
                     /gene="dksA"
                     /locus_tag="VNZ06_11635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HWW21450.1"
                     /translation="MAGKKPGSKPSKVKKPPSAKVAPRKVEPRKAATTASSRKAVAAT
                     SVKAEVKGARKPAVAHEHPGATAERRTPERAEAPIRAAAPEAAHPAKDPSVTARTRAL
                     HEAEEAEAESEGGEISSLLAGPRNVKPYVAKRGELYMSKEQLTHFQHILSTWKRDLME
                     EVDRTVSHMKDEAANFPDPNDRATQEEEFSLELRTRDRERKLIRKIDEALKRIEDGTY
                     GYCLETGEEIGIKRLEARPVATLSIEAQERRERRERQYGDRDDRYR"
     gene            3653..4093
                     /gene="phaR"
                     /locus_tag="VNZ06_11640"
     CDS             3653..4093
                     /gene="phaR"
                     /locus_tag="VNZ06_11640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HWW21451.1"
                     /translation="MSELRIIKKYPNRRLYDTVESRYITLDDVRRLVLGRIDFCVIDK
                     RTQQDITRPVLLQVIAEQEQRNGTILSQDFLSELIRSHDVGLEGMLSSYLEQNLRLFL
                     SEKPPIGEPAAAASHLANQNYQRWHSAQEEIFRTLMRAANATPS"
     gene            complement(4142..4285)
                     /locus_tag="VNZ06_11645"
     CDS             complement(4142..4285)
                     /locus_tag="VNZ06_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HWW21452.1"
                     /translation="MVHISQISDERVERVSDKLKEGDQIRVKVLEVDRQGRVRLSMRN
                     VDA"
BASE COUNT          792 a         1434 c         1328 g          748 t
ORIGIN      
        1 ccacggcctc ctgctcgagc tgcaccacgc gcgcatccgc ggcgccacac agttcgcgca
       61 gccccttcag acgatgctcg cgctcgagca cgccggcgtg cacatcaccg ccgcgattga
      121 cgcgcagcca gtcgcgccca acccattcac cggccgcagt gatcagcgac tcgccgttcg
      181 gcagctgcga gcgccgctgc agcgcggccg ccagcgacgg cgcggtatgc acacgctcca
      241 ggacgttgag cgccgcgttc ggaccgcgca cctgctcggc cagcgttccg gcggtggcct
      301 tcgcaccggc agtcaggtgc tcaaagaaag tcagatgccc cgtgtcgagg cccggaagcg
      361 aggattcgag ttgctcgagc gtgtccacgc cgaccgcctc cagatagtct ccgagcacgg
      421 tctcgactgc ccgctcccaa cccgactgca catcgatggt ctcggccagc ctcggccggg
      481 gtgtgcggcc gagcgccgca agccaggcgc cgatgcgtgg attcttttcg cgcagcgcgg
      541 ctttttgcag cgcctcgagc gacatcagtt ccgagcgtgc ctggtcgcgc tcgcgccgca
      601 ggcgctccac gcgtgcatcg gtgctctgct gcgaggcacg cagctgctgc acctgattga
      661 gcgcggactc cagttcacgg ccgagcgcat cggcggcgct gcgggcggcc gcctcgcgct
      721 cactcaagcc atcgatcggt gagtcagcgt gctgcgaagt gagtgaatcc tgctcgagct
      781 ccagacgatc ggcctgcgcc cgcaggcgat gccactggct ctcgagctgt tcgatcctgg
      841 ccgattcgac ctgctcgctt tgactggcgg cgccgagctc gcgattgaaa ttctcccagt
      901 tctgctgcca ggtggccagt tccgcctctg catcagcgag cgcaccggcg gagctctgtt
      961 cgctgccgcg tcgcgcctcg acctgtggct ccagcgtggc aatctccgcg cgcaacgcgg
     1021 ccagctgctg ctcgtcgtgc gcgatctgct gcgcgatgga ggtcagcgac acattcacct
     1081 gtgccagatc gttgcgtgcc cgctcgcgca gctcgcgcgt gtaacggatg ttctcctccg
     1141 cgcgcgtgac ctcggctccc agctcgtaga aacggccctg tacacgcgag gccgtatcgc
     1201 tcaattcggt atggaactcg cgctggcgtt cgatcgccgc ctcggccgag cgctgatcgg
     1261 ccagggcctg ctgcatcgtc agctcgcact cgcgcaccgc gctgtcctgc acggccgctc
     1321 cgctgtccag ctcgcgcagg cgcaacgcca gcagttcggc ggtcagagtg cgctcctgcg
     1381 ccttgagctc ctgataacga cgtgcgacgt tggcctgacg ctgcagatga cggatctgct
     1441 tgtccacctc atcacgcaga tcctgcagtc gcgccaggtt ctcgcgcgta tcgaccatgc
     1501 gcgactcggt ctcgcggcgc cgctccttat aaagagagat gcccgcggcc tcttccacga
     1561 aggcgcgcat atcgtcagcg cgcgcttcga tgacccgaga gatcatgccc tgctcgatga
     1621 tggcgtagct gcgcgagcct agacccgtgc cgaggaacag ctgcgtgatg tccttgcgcc
     1681 ggcagcgacc gccgttgatg aaataagcgg aactgccatc gcgtgagacc gcgcgcttga
     1741 gcgagatctc gctgtaggcg gcgtaagcac cgccgatctt gccgtcactg ttgtcgaaca
     1801 ccagttcgac cgaggcattg ccgaccggct tgcgggcact cgagccgttg aatacgacgt
     1861 cggccatcga ttcgccgcgc aggtgccggg ccgacagttc gcccatgacc cagcgcacgg
     1921 catcgatgat gttggatttg ccgcagccgt tggggccgac gacaccagta agatttccgg
     1981 gcagatgaac agaagtcggg tcgacgaaag atttgaatcc ggcgagtctg actttgttta
     2041 gtcgcaattt actagcatcc ggttcctggc agtcaagtat cccttggcga gctggcgcgc
     2101 cggtagtgta gattaagatg acagatattt gaacgccaat gccgactcag ccttcaaccg
     2161 agatcaaaac ctttcccaat ccgactccgg agcgcgacta cacgatccgg atgcgcattc
     2221 ctgagttcac ctgcctgtgt ccgatgacgg gtcagcctga cttcgccacg ctcgaactcg
     2281 aatacgtccc cgatcgggcg tgcgtcgagc tgctggcgct gaaactctac atctggtcgt
     2341 tccgcaaccg gggcgctttc cacgaagcgg tgaccaacga gatcctcagc catctggccg
     2401 acgccgtctc gcctcgcttc atgcgtctga gcgcggtgtt caatgtccgt ggcggcatct
     2461 acaccacggt cgttgcagaa cggcgcgcag cggattggcg gccggccgag cccgtccggc
     2521 tgccctagag cgcggtccca cgccccaggg cgacccctga aggcggtaaa ttgctgccaa
     2581 gtcagcattt ttctgactct gtacagtccg atttgcgtcg gtataatcgc gcgttcttta
     2641 aacgaccggc gaatgtcagg agcgattcga tggccggcaa gaagcccgga tccaaaccga
     2701 gcaaagtgaa aaaacccccc agtgcaaagg ttgccccgcg caaggtcgaa ccgcgcaagg
     2761 ccgccaccac ggccagcagc cgcaaagcgg tggcagcgac ctccgtcaaa gctgaggtga
     2821 aaggcgccag gaaacccgct gtcgcgcacg aacacccggg cgcgacggcg gagcggcgca
     2881 cccccgagcg agcggaggct cccattcgcg cggcggcgcc tgaggcggcg caccctgcga
     2941 aggacccctc ggtgactgcc cgtacccgcg cgctgcatga ggctgaagag gcggaggctg
     3001 aaagcgaggg cggggagatc agcagcctgc tggccggacc ccgcaacgtc aaaccgtacg
     3061 tcgccaagcg tggcgagctg tacatgagca aggaacagct cacccacttc cagcacatcc
     3121 tttccacctg gaaacgcgat ctgatggagg aagtggaccg cacggtctcg cacatgaagg
     3181 atgaggcggc caatttcccc gatccgaacg accgggccac tcaggaagag gaattcagcc
     3241 tggaactgcg cacgcgcgat cgcgagcgca agctcatccg caagatcgat gaggcgctga
     3301 agcgcatcga ggatggtacc tacggctact gcctcgaaac cggcgaggaa atcggcatca
     3361 agcgcctgga agcccgaccg gttgccacgc tgtcgatcga agcgcaggag cggcgcgaac
     3421 ggcgcgaacg tcagtacggg gatcgggacg accgctatcg gtagtcgcag gccggcgccc
     3481 acgccggccc gtcagctgat ctcctaccga ttcgaagcca ggcaccccac acgaggtgtg
     3541 tgatcggctg cccattcgac aatacagtaa atttgggttc cggccgcgct tgcttgtact
     3601 atgcgtcagg gctatgaggg tctgcgtgct ttgatcacac gggggcttcc ttatgagcga
     3661 gctgcgcatc atcaagaaat atcccaaccg tcgactctac gacacggttg agagccgcta
     3721 tatcacgctc gacgatgttc ggcggctggt actcggtcga atcgacttct gcgtcatcga
     3781 caagcgcacc cagcaggaca tcacgcgacc tgtgttgctg caggtgattg ccgagcagga
     3841 acagcggaat ggaacgattc taagtcagga ctttctgtcg gagctgatcc gcagtcacga
     3901 cgtcgggctg gaaggcatgc tctcgagcta cctggagcag aatctgcgtc tgttcctgag
     3961 cgagaagccc ccgatcggcg aaccggcggc cgctgcgagt cacctggcga atcagaacta
     4021 ccagcgctgg cacagtgcgc aggaagagat cttccgcacg ctgatgcgag cggccaacgc
     4081 gacgcccagc taaatcgcca gctaaatcgc ccggctgata cgccgcccgg ccgtggagcg
     4141 gctaggcgtc cacgttgcgc atggacagac gcacgcggcc ctggcgatcg acttccagga
     4201 ccttcacccg tatctgatcg ccttccttca gcttgtcgga cacgcgctcg acgcgctcat
     4261 ccgagatctg cgagatgtgc accagaccgt cgcgcccggg ga
//
LOCUS       DATMRM010000386         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1467680, whole genome shotgun sequence.
ACCESSION   DATMRM010000386 DATMRM010000000
VERSION     DATMRM010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467680"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VNZ06_11650"
     CDS             <1..571
                     /locus_tag="VNZ06_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWW21453.1"
                     /translation="VGLAFPSVVQGGIARTAANVDKTWIGCAGGARLSQLIGRPVVFL
                     NDADAAGVAEMSVGGAGAGVKGVVILLTFGTGIGSALFLDGELVPNTELGHMEFHGQD
                     AEYTASARVRTEERLDWAAWCARVNDYLARLHALFWPDLFILGGSISEDFDQYQHLLK
                     SPTPIRRAVHGAQAGVIGAAFAAAHQTQR"
     gene            576..1739
                     /locus_tag="VNZ06_11655"
     CDS             576..1739
                     /locus_tag="VNZ06_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HWW21454.1"
                     /translation="MAFRSKLPQVGTTIFTVMSRRATELQAVNLGQGFPDYDIDPRLA
                     ELVHEAMLQGHNQYAPMPGMPQLLRAIAAKLERSYGVQVDPEREITVTLGATEAIYSA
                     VQALVAPGDEALVFDPCYDSYEPAIQCSGAACIHVPLQPPQFRYDWERLKAALTPRTR
                     LLIINSPHNPACTCMTAQDLSRLAELLRDRGIYLISDEVYEHIVFDGRKHQSVLSSPE
                     LRARSVAVYSFGKTLSATGIRVGYAVAPAGLTTELRKVHQFNTFTIATALQVAIARYL
                     DERPDTGLGLSGFFAAKRDRLIAALRGTPFKATAAEGSFFQLIDYAERSRATDVEFAD
                     ELLTRAGVASIPLSVFYQQPPPMTLLRLCFAKRDETIDRAAARLRDYARGGSA"
     gene            1736..>2765
                     /locus_tag="VNZ06_11660"
     CDS             1736..>2765
                     /locus_tag="VNZ06_11660"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HWW21455.1"
                     /translation="MRKPTLVNHPPAVPVPADNRPVVAPIHQTVKFEFETVDETLRSL
                     RGDRPGFFYLRSSNPTTRQLELTLAQLQGREECVVCASGVGAIAQTLLSLTRTGDHVL
                     CFIETYGPTRQLIRRMLSKFGVTHTMLSIEDLAGIERVLASHPTRLIVFESPTNPVNK
                     IADLAAITRLARTHGALTLLDNTFAGVHQHGQFEIDIYLHSLTKFVSGHGDVMGGAAI
                     ANAGIIRTLRPDFTLLGGVLDPHAAFLLQRGLKTYFVRYRAQCASAQRIAEFLCTHPA
                     VERTHYPGLLGAASNALARAQMSEFGAVLSFDLRSGAAGGRVFAESLELFSMAASLGS
                     TESLVMPPQ"
BASE COUNT          471 a          885 c          883 g          526 t
ORIGIN      
        1 ggtgggtctg gcatttccgt ccgtggtaca gggagggatt gcgcgcaccg ccgccaacgt
       61 cgacaagacc tggatcggct gtgcaggcgg cgcacgactg tcgcagctga tcggccgtcc
      121 ggtggtattc ctcaacgacg ccgatgcggc cggtgtcgcc gagatgtcgg tcggtggcgc
      181 gggcgcggga gtcaaaggtg tcgtcatcct gctcacgttc ggtaccggta tcggctcggc
      241 gctgtttctg gacggggaac tggtgccgaa caccgaactc ggtcacatgg agtttcacgg
      301 tcaggatgcc gaatacaccg cctcggcccg ggtacgcacc gaggagcgtc tcgactgggc
      361 cgcctggtgc gcgcgcgtga acgactatct ggcccggctg cacgcactgt tctggccaga
      421 cctgttcatt ctcggaggat cgatcagtga ggacttcgat caataccagc atctgctgaa
      481 gagtccgact ccgatccggc gtgcagtcca tggcgcgcaa gccggcgtga tcggcgctgc
      541 gttcgcggcg gcacatcaga cgcagcgtta ggcgcttggc gtttcgcagc aagctgccgc
      601 aggtcggcac cacgatcttc acggtgatgt cgcgccgcgc aacggaactg caggccgtca
      661 atctgggcca aggcttcccg gactacgaca tcgatccgcg cctggcggag ctggtccatg
      721 aggccatgct tcaggggcac aatcagtacg cgccgatgcc gggaatgcct cagctgctac
      781 gcgcgatcgc cgccaagctc gagcgcagct atggcgtgca ggtcgatccg gagcgggaaa
      841 tcaccgtgac gctgggtgcg accgaggcca tctactccgc tgtccaggct ttggtggcac
      901 ccggggatga agcgctggta ttcgatccct gctacgactc ctacgagccg gcgatccagt
      961 gctcgggcgc cgcctgcatt cacgtgccgc tgcagccacc gcagtttcgc tatgactggg
     1021 agcgcctcaa agccgcgctc acaccgcgta cgcgtctgct gatcatcaac tctccccaca
     1081 atccggcctg tacctgcatg acggcacagg atctgtcaag gcttgcggag ctgttgcgtg
     1141 accgcgggat ctacctgatc tccgatgagg tttacgaaca catcgtgttc gacggtcgca
     1201 agcatcagag cgtactgagt tcgccggaac tgcgtgcccg cagcgtcgcg gtctattcgt
     1261 tcggtaagac actgagtgcc acgggcatcc gtgtgggcta cgcggtggcg cctgcggggc
     1321 tcaccaccga gctgagaaag gtgcaccagt tcaatacctt caccatcgcc accgcgctgc
     1381 aggtcgcgat cgcgcgctat ctcgatgagc gtcccgacac cgggctcggg ctgtccggat
     1441 tttttgccgc caagcgcgat cgcctgatcg cggcgctgcg tggcacgccc ttcaaggcca
     1501 ctgccgccga aggctcattc tttcagctga tcgactacgc tgaacgcagc cgtgcgaccg
     1561 acgtcgagtt cgcggacgag ctgctgacgc gggccggagt tgcaagcatt ccgctgtcag
     1621 tgttctacca gcagccaccg ccgatgacgc tactgcgcct gtgctttgcc aagcgcgatg
     1681 agacgattga ccgggctgca gcccggctgc gcgactacgc gcgaggaggc agtgcgtgag
     1741 aaaacccacg ctggtgaacc atccgcccgc ggtaccggtt ccggccgaca accggccggt
     1801 ggtcgcgccg attcatcaga cggtcaaatt tgaattcgag acggtcgatg agacgctgcg
     1861 ctcgctgcgc ggcgatcggc cgggcttctt ctatctgcgc agttccaatc ccaccacgcg
     1921 acagctcgag cttacgctcg cgcagctgca gggccgggaa gagtgtgttg tgtgtgcctc
     1981 gggagtcggc gctattgctc agacgctgct gagtctgacg cgtaccggcg atcacgtcct
     2041 gtgtttcatc gaaacctacg gcccgacgcg ccagctcatc cgccgcatgc tctcgaagtt
     2101 cggcgtcacg cacaccatgt tgtcgatcga ggatctggcc ggtatcgagc gggtgctggc
     2161 atcgcacccg acgcgactga tcgtgttcga aagtccgacc aatccggtca acaagatcgc
     2221 ggaccttgcg gcaatcacgc gcctcgcacg cacgcacggc gcgctgacct tactcgacaa
     2281 taccttcgcc ggcgtgcacc agcatgggca gttcgagatt gatatctatc tgcacagcct
     2341 caccaagttc gtctccggac acggcgatgt gatgggaggt gcggcgatcg cgaacgccgg
     2401 aatcatccgg acgctgcgcc cggatttcac attgctgggc ggtgtgctgg atccgcacgc
     2461 ggcgttcctg ttgcagcggg gcctgaagac ctacttcgta cgctaccgcg cgcagtgtgc
     2521 cagtgcgcaa cggatcgccg agttcctgtg cacgcacccg gcggtcgagc gcactcacta
     2581 tccgggtctg ctgggagcgg cgtccaatgc gctggcacgc gcccaaatga gtgaattcgg
     2641 cgctgtgctg agtttcgatt tgcgctcggg cgcagccggt ggccgcgttt tcgcggagag
     2701 cctggaactg ttctcgatgg ccgccagcct cggttcaacc gagtcgctgg tgatgccgcc
     2761 gcaat
//
LOCUS       DATMRM010000387         3007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1804524, whole genome shotgun sequence.
ACCESSION   DATMRM010000387 DATMRM010000000
VERSION     DATMRM010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1804524"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VNZ06_11665"
     CDS             <1..844
                     /locus_tag="VNZ06_11665"
                     /inference="COORDINATES: protein motif:HMM:NF019944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HWW21456.1"
                     /translation="DSAVVRSEQNSEESSRGGAGNGGIPGALTNQPPAGGTAVAAGAK
                     PATPPAPGAGGKNANAAADAAAAAAAAAAAATPQNSSKQSTKNYEIDRTLSYARQPAG
                     RIKRVSVAVLVDNVHQSSEDGTAKDRALTKEELEGITKLVKDTVGFDEARGDSVNVVN
                     QPFLPHTEEPIKPETIPLWQKPMVRDIARLVLGALVLVALALGVLRPLIKNITASLPA
                     ASSPALLSVGEGAAGGVAASVPVVAAVPGQPAPYEQQLVQARTMVAQDPKRVAQVVKT
                     WVGE"
     gene            845..1861
                     /gene="fliG"
                     /locus_tag="VNZ06_11670"
     CDS             845..1861
                     /gene="fliG"
                     /locus_tag="VNZ06_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HWW21457.1"
                     /translation="MAETPPIPRSGVDRAAILLLALGETDAAQVLKHLGVKDVQRVGA
                     AMAQLSGVSRDEVQGILTNFSSTVDQQTSLGVGNDDYVRKVMVEALGADKAAGVVDRI
                     LRGRSGRGLESLKWMEAKSVAEMLRFEHPQIISIVLSYLESDQAAEVLTVLPENMRGD
                     ILMRIATLEGVHPTALNELDEVLERQFAGGGAPKASSGFGGPKAAAGILNLVGTALEG
                     TILAQIAKFDEPLSVKLQELMFVFEDLIEVDDRGMQELLREVPGDKLIIALKAADEKL
                     KEKFFKNMSERAAQMMKDDLESKGPVKLSDVEAAQKEILMTTRKMADEGRLQLGGKGG
                     EEYV"
     gene            1854..2483
                     /locus_tag="VNZ06_11675"
     CDS             1854..2483
                     /locus_tag="VNZ06_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HWW21458.1"
                     /translation="MSDKAVASAWELPPIDGPVLARRRRVEQLDALEREAWEKGYAEG
                     REAGTAAVRQEEQAALAEIERRSRQIASILDFMAKPIADLDDQVQRQLALLAGAIARQ
                     VVRRELKTHPDQIIAAIRETVSLLPMTARDVRVHLHPEDAKLVRTRLAEATSERAWTI
                     TEDPILTQGGCRVTSEDSTIDAQVEARIGAAIAAVLGDERSAVAGRPTP"
     gene            2480..>3007
                     /locus_tag="VNZ06_11680"
     CDS             2480..>3007
                     /locus_tag="VNZ06_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21459.1"
                     /translation="MNPVYTLAAQRWTHGLARFQERLANVAPPVVAGRLTRMVGLTLE
                     AAGCQAALGDRCEIVSTDNNKIEAEVVGFASDRLYLMPTGDVHGLKPNARVLPRPHAG
                     TVPVGPKLLGRVVDGACLPLDGRGPIETETSVRLSGVPINPLMRRPITQPLDVGVRAI
                     NGLLTIGRGQRVGLFA"
BASE COUNT          569 a          904 c         1045 g          489 t
ORIGIN      
        1 cgacagcgcc gtggtacgca gcgaacagaa ctcggaggag tcatcgcgtg gcggcgccgg
       61 caatggtggc atccccggcg cgctgaccaa tcagcctccg gccggaggca cggcggtcgc
      121 cgccggtgcg aagccggcaa ctcctcccgc tcccggtgcc ggaggcaaga acgccaacgc
      181 ggcggccgat gcggccgctg ctgccgcagc cgccgcggcg gctgcgacac cacagaacag
      241 ctcgaagcag agcaccaaga actacgagat cgatcgcacg ctcagctatg cgcgtcaacc
      301 cgccggtcgc atcaaacgcg tctcggtggc ggtgctggtc gataacgtgc accagagcag
      361 cgaggatggc acggccaagg atcgagcgct caccaaggaa gagctcgaag gcatcaccaa
      421 gctggtgaag gataccgtcg gtttcgatga ggctcgcggc gacagcgtca acgtcgtcaa
      481 ccagccgttc ctgcctcata ccgaagagcc gatcaaaccc gaaacgattc cgctgtggca
      541 gaagcccatg gtgcgcgaca tcgctcgtct ggtactgggc gcgctggtac tggtcgcgct
      601 cgctctgggc gtgctgcgcc cgctgatcaa gaacattacc gccagtctgc ctgcggcatc
      661 ctcgccggct ctgctatcgg taggggaagg cgcagcgggt ggcgttgcgg cgagcgttcc
      721 ggtcgtcgca gcagtgcccg ggcagccggc gccgtatgaa cagcaattgg tccaggcgcg
      781 cacgatggta gcgcaggatc cgaagcgggt agcgcaggtc gtgaagacct gggtgggtga
      841 ataggtggct gaaactcctc cgatcccgcg ctcaggcgtg gatcgcgccg cgattctgtt
      901 gctggccttg ggtgagacgg atgccgcgca ggtgctgaag catcttggcg tcaaggatgt
      961 gcaacgcgtg ggcgccgcga tggcgcagct ctcaggggtt tcgcgcgatg aagtgcaggg
     1021 catactgacc aacttttcca gtaccgtcga ccagcagacc tcgctcggcg tgggcaacga
     1081 tgactacgtg cgcaaggtca tggtcgaggc gctgggggcc gacaaggccg ccggcgtggt
     1141 cgatcggatt ctgcgcggcc gcagcggtcg cggactcgag tcgctcaaat ggatggaagc
     1201 gaagtcggtc gcggagatgc tgcgcttcga acatcctcag atcatctcca tcgtgctgtc
     1261 ctatctcgag tcggatcagg cggcggaagt tctgacggtc ctgcccgaga acatgcgcgg
     1321 cgacattctg atgcgtatcg cgacgctcga aggggttcat ccgacagcgt tgaacgaact
     1381 ggacgaagtg ctggaacgac agttcgccgg tggcggggca cccaaggcat ccagcgggtt
     1441 tggcggaccg aaagcggcgg ctggcattct gaatctggtc ggtacagcgc tggaaggcac
     1501 catcctcgcc cagatcgcga aattcgatga gccgctgagc gtcaagctgc aggaactgat
     1561 gttcgtgttc gaggacctga tcgaagtgga cgatcgcggc atgcaggaat tgctgcgcga
     1621 agtgccgggc gacaagctga tcattgcact caaggccgcg gacgagaagt tgaaggagaa
     1681 attcttcaag aatatgtccg agcgcgcagc gcagatgatg aaggacgatc tggaatccaa
     1741 ggggccggtc aaactgtccg atgtcgaggc ggcgcagaaa gagattctga tgactacgcg
     1801 caagatggcc gacgagggcc gcttgcagct cggtggcaag ggcggagagg agtatgtctg
     1861 acaaggcagt cgcctcggcc tgggaattgc caccgatcga cggcccggtg cttgcgcgtc
     1921 ggcggcgggt cgagcagctg gatgcgctgg agcgcgaggc ctgggaaaag ggctatgccg
     1981 aaggccgcga agcgggcacc gcagctgtgc gccaggaaga gcaggcggct ctggcagaga
     2041 tcgagcgacg ctcgcgacag atcgcatcga tcctggactt catggccaag ccgatcgccg
     2101 atctggacga tcaggtgcag cgccagctgg cgttgctcgc gggagcgatc gcgcgtcagg
     2161 tcgtgcgccg cgaactgaag acccaccccg atcagatcat tgccgcgatc cgcgagacgg
     2221 tatcgctgct gccgatgacc gcgcgtgatg tgcgtgtgca cttgcatcct gaggacgcga
     2281 agctggtgcg aacccgcctg gccgaggcca ccagcgagcg tgcctggacg attactgaag
     2341 atccgatcct cacccagggc ggctgccgcg tgacgagtga ggattctacg atcgacgcac
     2401 aggtcgaagc gcgcattggc gccgcgattg ccgccgtgtt gggcgatgag cgcagcgccg
     2461 ttgccggcag acccacgcca tgaacccggt ctacaccctg gcagcgcaac gctggaccca
     2521 tggactggcc cgctttcagg aacgcctggc gaacgtcgcg ccgcccgtgg tcgctggacg
     2581 tctcacacgc atggtgggcc tgaccctcga ggccgcgggc tgccaggcgg cgctcggcga
     2641 tcgctgcgag attgtgagca ccgacaacaa taagattgaa gccgaagtcg tgggattcgc
     2701 cagcgaccgg ctctatctga tgcctaccgg cgatgtgcat ggactgaagc ccaatgcacg
     2761 ggtgctgcca cgtccccacg ccggtaccgt tccggtgggc ccgaagctgc tgggtcgggt
     2821 cgttgacggc gcctgtctgc cgctggacgg ccggggcccg atagaaaccg aaaccagcgt
     2881 ccgcctgagt ggtgtgccca tcaatccgct gatgcgccgg ccgatcaccc agccgttgga
     2941 cgtgggtgtg cgggccatca atgggctgct gaccatcggc cgtgggcagc gggtcggtct
     3001 gtttgcg
//
LOCUS       DATMRM010000388         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_554070, whole genome shotgun sequence.
ACCESSION   DATMRM010000388 DATMRM010000000
VERSION     DATMRM010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554070"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VNZ06_11685"
     CDS             <1..379
                     /locus_tag="VNZ06_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21460.1"
                     /translation="TERVNGKVLWANLALLFWLSLVPFVIRWIDETGFTALPTAAYGI
                     VLTGAAMSYHVLQQQLIAYNGHDSRLAAAVGNDFKGKLSLILYLAAIVLAFVRPWMAI
                     TLYVAVALLWLVPDRRIESLVKK"
     gene            383..1246
                     /locus_tag="VNZ06_11690"
     CDS             383..1246
                     /locus_tag="VNZ06_11690"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWW21461.1"
                     /translation="MLERIETRAAEIGGGLIVRRALPNRRRRMVGAWCFLDHAGPMEF
                     GRGGGMGVGPHPHIGLQTFTWMIEGAVMHRDSLGNEQLITPGQVNLMTAGRGIAHAED
                     SDSAAGGRLHAVQLWIALPDAQRHCPPAFRNYPHLPLVELGGFSVRVLAGSAFGHTSP
                     AEVYSPLVGLDLVATGPARISLPLTASFEHAALVLSGAASIEGEELTPDTLLYLGSAR
                     ERLAVSCEAATRIILVGGVPFGEDVLLWWNFVARSAQEMEQAARDWNTGRRFGPVHGS
                     PSARLTAPARS"
     gene            complement(1257..2126)
                     /gene="purU"
                     /locus_tag="VNZ06_11695"
     CDS             complement(1257..2126)
                     /gene="purU"
                     /locus_tag="VNZ06_11695"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HWW21462.1"
                     /translation="MRFATDHPYVLTASCPNRMGTTAAITGFLAARRFYLIEMHQFDD
                     TISQRFFVRITFCGVAGEGVDLGRLRQEFQAIATAEEMEWRIHDTRDRTRVLIMVSNL
                     DHCLEDLLYRHRTGELKMDITAVVSNHVTLQPTAARHNLPFFHLPVTAGTKPEQERRL
                     LELIDSTRTELLVLARYMQILSDELCGRLRGRAINIHHSFLPGFKGAKPYHQAYDRGV
                     KVIGATAHYVTHDLDEGPIIEQIVERVDHGYTPDMLVAAGRDSERRALSAAVRYATEH
                     RVFLNGGRTVVFK"
BASE COUNT          378 a          769 c          793 g          422 t
ORIGIN      
        1 caccgagcgc gtcaacggca aggtgctgtg ggccaacctg gcgctgttgt tctggctgtc
       61 gctggtgccg ttcgtgatcc gctggatcga cgagacggga ttcactgccc tgccgacggc
      121 tgcttatggc attgtgctga cgggcgctgc gatgagctat cacgttctgc agcagcagct
      181 catcgcctat aacggccacg actcgcgcct ggctgccgcg gtcggtaacg acttcaaggg
      241 caagctgtcc ttgatcttgt acctcgccgc catcgtgctg gcgttcgtgc gaccgtggat
      301 ggcgatcacg ctgtatgtgg cggtggcgct gctctggctc gtgccggacc ggcgcatcga
      361 gtcgctggtg aagaagtaac gcatgcttga acgcattgaa acacgtgccg cggaaatcgg
      421 cggcgggctc atcgtcaggc gggcgctgcc caaccggcgg cgtcgcatgg tcggagcctg
      481 gtgcttcctc gatcacgccg ggccgatgga attcgggcgc ggcggcggca tgggcgtcgg
      541 accccatccg cacatcggac tgcagacctt cacctggatg atcgagggcg cggtgatgca
      601 ccgtgacagc ctcggcaatg agcagctgat cacgcccggc caggtcaatc tcatgaccgc
      661 gggccgcggc atcgcgcatg ccgaagattc cgactccgcc gccggcggcc gcctgcatgc
      721 cgtgcagttg tggatcgcgc tgcccgatgc gcagcgccac tgcccgccag ccttccggaa
      781 ctatccgcac ctgcccctgg tcgagctcgg cggcttcagc gtgcgggtgc tggcaggcag
      841 cgccttcggt cacacctcgc ccgccgaggt ctattcaccg ctcgtgggcc tggatctggt
      901 tgccaccggc cccgcccgaa tcagcctgcc cctgacggcc tcgtttgagc acgccgcgct
      961 ggtgttgagc ggcgccgcga gcatcgaagg tgaagagctg acaccggaca cgctgctcta
     1021 cctcggcagt gcccgcgagc ggctcgctgt gagctgcgag gcggccacgc gcatcatcct
     1081 ggtgggcggc gtgccgttcg gcgaagacgt cctgttgtgg tggaacttcg tggcacggag
     1141 cgcgcaggaa atggagcagg cggcgcggga ctggaatacc gggcggcgat tcgggcccgt
     1201 ccacggcagt ccttccgcgc gtctgaccgc cccggcccgt tcctaacgcg ccgcgcctac
     1261 ttgaatacca ccgtccggcc gccgttcaga aatacgcgat gctcggtcgc gtagcgcacc
     1321 gccgccgaca gcgcgcgccg ttcggagtcg cggccggctg cgaccagcat gtcgggcgtg
     1381 tagccgtgat cgacgcgctc gacgatctgc tcgatgatcg gaccctcatc gagatcgtga
     1441 gtgacgtaat gggcggtggc cccgatcacc ttgacgccgc ggtcgtaggc ctggtggtag
     1501 ggtttcgcac ccttgaatcc cggcaggaac gagtggtgaa tgttgatggc gcggccccgc
     1561 agtctgccgc acagctcgtc tgacaggatc tgcatgtagc gcgcgagcac cagcagctcg
     1621 gtgcgggtcg aatcgatgag ttccagcagg cgccgctcct gttcgggttt ggtgcccgca
     1681 gtgaccggca ggtgaaagaa cggcaggttg tggcgggccg cagtcggctg cagcgtcacg
     1741 tggttcgaca ccaccgccgt gatgtccatc ttgagttcac cggtgcgatg ccggtagagc
     1801 agatcctcca ggcagtgatc gagattcgac accatgatga gtacccgcgt acggtcgcgg
     1861 gtatcgtgga tccgccattc catctcctcg gcggtggcga tcgcctggaa ttcctgccgc
     1921 agccgcccca gatcgacccc ctcgcccgca acgccgcaaa acgtgatgcg cacgaagaag
     1981 cgctgcgaga tcgtatcgtc gaactggtgc atctcgatga ggtagaagcg ccgggcggcc
     2041 aggaaacccg tgatcgccgc ggtcgtgccc atgcggttcg ggcaactcgc ggtcagcaca
     2101 tagggatgat cagtcgcaaa cctcactcac ccgctcctcg cgccatgtcg gggtcgccag
     2161 cgtgacagcg cctccggccg gcctgtgatc caaatgggac ggcggctggc gcgtacgcgt
     2221 catggccacg cgaagatctc gatgaggatc cgcattcacg agtcgggaaa tgccaccgag
     2281 aaagctccgt cgcggctctg cggcgttccc ggctcgatct cgtagtagtc gcccttgtcg
     2341 gcgacgaaga tatggcaggc ga
//
LOCUS       DATMRM010000389         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3617179, whole genome shotgun sequence.
ACCESSION   DATMRM010000389 DATMRM010000000
VERSION     DATMRM010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3617179"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="VNZ06_11700"
     CDS             <1..984
                     /locus_tag="VNZ06_11700"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWW21463.1"
                     /translation="AILHLLYSRFWTRVMRDFSMVQLSEPFGRLMTQGMVLNHIYYRT
                     GANGRRQYIDPADVEPRFDANHLPVGGVSLTDHQPVEYGGMGTMSKSKGNGVDPQIFI
                     AKYGADTVRLFIMFASPPEQTLEWSEEGVQGQFRFLRRLWKAVYDHVSAGKACAIDKS
                     ALSSAARELRYETHHTLTRVTGDIGRRRHFNTAIAAVMKLLNALGAYDEDGPNAHAVR
                     QEALEIAVLALSPIVPHVAHALWHELGHTGAIIDERWPRADAEALRQTTVEVIVQING
                     KLRGKLQLPVGSQRELALEAAVTDPNVQRFIAGKQIRKVIHVPDKLVNLVI"
     gene            981..1481
                     /gene="lptE"
                     /locus_tag="VNZ06_11705"
     CDS             981..1481
                     /gene="lptE"
                     /locus_tag="VNZ06_11705"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HWW21464.1"
                     /translation="MRFACLALLLGLSGCGFHLEGSTPLPAALAVVRVDTSDRQTDFY
                     FGLRKALMAAGTRIDDDVKDGDGAVIHILTDSSPQPRTLTVSTLNVPTEYELTYTVRF
                     SVTVKGHEVIAPEEHFLVRDYSYSESAQLAKEREYAILSQALAHELVSVVMRRLASLS
                     GSVAHS"
     gene            1486..1968
                     /locus_tag="VNZ06_11710"
     CDS             1486..1968
                     /locus_tag="VNZ06_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21465.1"
                     /translation="MLLLSGTDRTAVTRLLLGYGLDLREIQPDENIPGSYWGESEAGL
                     RGTTLFARLDTPLHSILHESAHFICMSPERRVGLDRDAGGDDAEESAVCYLQILMAEA
                     LAGVGSTRLCADMDAWGYSFRLGRTALWFEQDAQDARAWLHRHGIIDDTGGLTGALRA
                     "
     gene            complement(1973..>2491)
                     /locus_tag="VNZ06_11715"
     CDS             complement(1973..>2491)
                     /locus_tag="VNZ06_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21466.1"
                     /translation="DDYQSLDRIVELATAEADRRILVDLAAQTHQPLSRWMEESRLLD
                     LTQQFNITLMYWHVMDNSFDSVELLRRLLDQFGDKLKLVLVLNHIRGERFELLEASCQ
                     RTRAEQSGAKIVALQRLADATMHKLDADSRSFWSAMNHAAGADGNLGMLERQRVWVWM
                     KRAYEEIDQVGV"
BASE COUNT          449 a          763 c          815 g          464 t
ORIGIN      
        1 gcgatcctgc acctgctgta ctcgcgtttc tggacgcgag tgatgcgcga tttctcgatg
       61 gtgcagttat ccgagccgtt cggccggttg atgacccagg gcatggtgct caatcacatt
      121 tactatcgga ccggcgccaa cggacggcgg caatatatcg acccagccga cgtggaaccg
      181 cgtttcgatg ccaatcatct accggtcggc ggggtgtcgt tgaccgacca tcagccggtc
      241 gagtatggcg gcatgggtac gatgtcgaag tcgaaaggca acggcgtcga tccacagatc
      301 ttcatcgcca agtacggcgc cgataccgtg cggctgttca tcatgttcgc ctcgccacca
      361 gaacaaaccc tggaatggtc cgaggagggt gtgcagggcc aattccgctt tctgcgccgc
      421 ttgtggaagg cggtctatga tcatgtctcg gcaggcaaag cgtgcgcgat cgacaagtct
      481 gcgttgtcga gcgcggcacg ggagctgcgc tacgagacgc atcacacgct gacgcgggtg
      541 actggcgaca tcgggcggcg ccggcacttc aacaccgcga tcgccgcggt catgaagctg
      601 ttgaacgcgt tgggcgccta cgacgaagac gggcccaatg cgcacgcggt gcggcaggag
      661 gcgctggaaa tcgcagtgct cgcactgtcg ccaatcgtgc cgcacgtcgc gcatgcgctg
      721 tggcatgagc tcgggcatac gggagccatc atcgatgagc gctggccgcg ggccgacgcc
      781 gaagcgctgc ggcagacgac ggtcgaggtc atcgttcaga tcaacggcaa gctgcgcggc
      841 aagctgcagt tgccggtcgg ctcgcagcgt gaactcgcgc tggaagcggc ggtaaccgat
      901 ccgaacgtgc agcgcttcat cgccggcaag cagatccgca aggtcattca cgtgccggac
      961 aagctggtca atctggtcat atgagattcg cgtgcctcgc gctgctgctc ggcttgagcg
     1021 gctgtggctt tcatctggag ggcagcacac cgttgccggc cgcgctcgcg gtcgtgcgcg
     1081 tggacaccag cgatcggcag accgatttct atttcggctt gcgcaaggcg ttgatggccg
     1141 caggtacacg gatcgacgat gacgtcaagg atggggatgg cgcggtgatc cacatcctca
     1201 ctgactcctc accgcagccg cgcacgctga cggtatccac gctcaatgtc ccgaccgaat
     1261 acgagctcac ctacacggta cgcttctcag tcacggtcaa aggccacgaa gtcatcgcac
     1321 ccgaggagca cttcctggtg cgcgactaca gctacagcga aagcgcgcag ctcgccaaag
     1381 agcgcgaata cgcgatcctc agccaggcgc tggcgcacga gctggtttcg gtggtgatgc
     1441 ggcgcttggc gagcctgtcg ggcagcgtag cgcattccta gcgccatgtt gctgttgtcc
     1501 ggcaccgatc gcaccgccgt gacgcggctg ctgcttggct atggcctcga tctgcgtgag
     1561 atccaaccgg acgagaatat tcccggttcc tactggggcg agagcgaggc tggtttgcgc
     1621 ggcaccaccc tgttcgcgcg gctcgacacg ccgctgcact cgatcctgca cgagagcgcg
     1681 catttcattt gcatgagccc ggagcgtcgc gtcgggctcg atcgcgacgc cggtggcgac
     1741 gatgcggagg aaagcgccgt gtgctacctg cagatcctga tggccgaggc gctcgcagga
     1801 gtgggcagca ctcggttgtg cgccgacatg gatgcgtggg gctacagttt tcgtctcggc
     1861 cggaccgcgc tgtggttcga gcaggatgcg caggatgcgc gcgcctggct gcaccggcac
     1921 ggcatcatcg acgatactgg cggcctgacc ggagcgctgc gcgcctagcg tcctagacgc
     1981 cgacctggtc gatctcttcg tacgcgcgct tcatccacac ccacacgcgc tgacgctcga
     2041 gcatgccgag attgccatcg gcaccagcgg cgtgattcat cgccgaccag aagctgcggc
     2101 tgtcagcgtc gagcttgtgc atcgtggcat ccgccagacg ctgcagcgcg acgatcttgg
     2161 cgccggactg ttcggcacgg gtacgttggc acgaggcttc gagcagttcg aaacgctcgc
     2221 cgcggatgtg attgagtacc agtaccagct tgagtttgtc gccgaactga tcgagcaggc
     2281 ggcgcagcag ctcgaccgag tcgaagctgt tgtccatcac gtgccagtac atcagcgtga
     2341 tgttgaactg ctgggtcaga tccagcagac gcgattcttc catccagcgc gacagtggct
     2401 ggtgggtctg tgcggccaga tcgaccagga tacggcgatc ggcttcggcc gtagccagtt
     2461 cgacgatccg gtcgagactc tgatagtcgt c
//
LOCUS       DATMRM010000390         7227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_555090, whole genome shotgun sequence.
ACCESSION   DATMRM010000390 DATMRM010000000
VERSION     DATMRM010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7227
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555090"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2167
                     /locus_tag="VNZ06_11720"
     CDS             <1..2167
                     /locus_tag="VNZ06_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWW21467.1"
                     /translation="PRASSVNIYDERGELLWATHLSMSLELTTLVQESIADAIENPSV
                     TGRFKQMGEVPVYLFWLRREDPANGPPLASLVVRLKAGGNIESCSFGFVQQIVQPALD
                     VLRQDLLAREEIQRLRDSMQEQTQELKVLEVVAGRGTETGQPGAEDLKSILANSAKHM
                     KVGMAALIVPDKGLAMTVAGDGGKLDYRLLAKAHRHLVSVARMRREPLMINKVSLPGQ
                     EQSEYRILAAPVFRPDGHAIGVFALFRTSSSPEFTASHSRVIELLARRLASVVAYEYD
                     ELSGLLTRQAFERRMKQILQDPKRAQSEWTALYIDIDHMHAINDAHGMHNGDRILKQL
                     GELIRRHIPPGALATRVSADRFAILLPARLDDSVKVGEALCQNAPQLSASLGVDKVSV
                     SVSVGVARVESMEQDFTHVLAHAETACKAAKARGANRVESYQEDNESIVRRMTELRLV
                     ANLQRALAENTLQLFAQLIAPLGDRQRPPQFELLLRLIDEKGKRVGPDQFMSAAQRHE
                     LMPAIDRWVIQTTLESLKPHASLLAQDLVGFSVNVSGHALRDASFAEDIESVIRASGL
                     APGGLCFEISEDAMMGNLAPAENFMRRLSKLGCRIALDDFGTGLSALAYLRTLPIGTL
                     KIAGSFVRDVLVDSKADSMVASIAHLAHSMGLTTVAKQVETEEVCTRVASLGADYAQG
                     FSIASPVPLREILSELPLYTTPAKPYLAVQMASGKIGNA"
     gene            2318..2746
                     /locus_tag="VNZ06_11725"
     CDS             2318..2746
                     /locus_tag="VNZ06_11725"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWW21468.1"
                     /translation="MPDRVLDRGLRQPSACGDHLQTRTNPSLAPIGAVSQNRALKFYT
                     QQLGFEVATDQAFDEKQRWIELRVAHSHTRVVLFTPEGHEDRIGTQFNGSLACDDVDA
                     TYRQLRQRGVEFIAEPQKQPWGTFAVFKDPDGNQFVLSSA"
     gene            complement(2782..3336)
                     /locus_tag="VNZ06_11730"
     CDS             complement(2782..3336)
                     /locus_tag="VNZ06_11730"
                     /inference="COORDINATES: protein motif:HMM:NF019819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HWW21469.1"
                     /translation="MHLFRAALAAPLLSILLLACASTRSPVRTVDQVDLPRYMGDWYV
                     IAEIPNFAEKHCVDSVESYALRADGGIDNWFSCRKKSFDAPMKRITSARAMIADQRTN
                     ATWHLRFLKVISVNYFILDLDPNYRWVMIGHPSRSYGWVLARTKDLPDALYQSILERA
                     HSQGYDPTKFIRVPQYPTDAAGAR"
     gene            3422..3859
                     /locus_tag="VNZ06_11735"
     CDS             3422..3859
                     /locus_tag="VNZ06_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21470.1"
                     /translation="MAKVANTITPEYLRKLRLYELAIAMVPGLVRKGASVPYTAVNGN
                     MSSYLHPRGEVALRLAEQQRKEFLKQYKARLFEAYGIVQKEYVTVPEALLADTMALGA
                     HFRASLDYVGGLKAKPPKKKAAQNKTVKSRAGNRTRSAAKRKR"
     gene            3892..5214
                     /locus_tag="VNZ06_11740"
     CDS             3892..5214
                     /locus_tag="VNZ06_11740"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWW21471.1"
                     /translation="MKTRRMVGYCVASLAMGIASQLATAAGAPGTAVPIALKAAHLFD
                     SISGNLIDHGVVVISGNKIQSVGADLKIPADAKVIDLGDATLLPGFIDAHVHLDSEMT
                     SNYYLDAYEGIMRFPAEQALHGARNAKVTLEAGVTTVRDLGSTDYISLGLRNAIRDGL
                     IPGPRMLIANYAIGSTGGHADQDPYPPQRIAVAGPIQGVCNGPDECREAVRYQIKYGA
                     DVIKFMPSGGVLSLSDPVDNAQLTQEEMNAIVTEAHNWGRKVAAHCHGDKAAKMAIAA
                     GVDSIEHGSFLKDDTLLEMKRRHVYLVATLFAGFWANGHADTFPAPIAAKAHAAAAQA
                     QLMFQHAVKLGVPLAMGTDAGVEPHGLNAREFSLMTENGLSAAQALIAGTANSAELLG
                     LSDLTGTLAVGKLADIIAVAGNPLTDMQTTEHALFVMKEGSIYVSPTR"
     gene            complement(5211..5852)
                     /locus_tag="VNZ06_11745"
     CDS             complement(5211..5852)
                     /locus_tag="VNZ06_11745"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWW21472.1"
                     /translation="MEKPEIIGSLRSSYTRAVCMACLEKGVPYTLTQSLLGGPEVLAI
                     NPFGKMPVLRHGDFELYESVAIAVYLDRVFTGPALFPSDPRQLARALQWICVVNSLLY
                     GTFRSYLLAVIAPQTADGAPDLAAIEALKPPLQLQLSVLDGALTANHYLAGESLSFAD
                     LNLVTILHNLRLLPGGPEMLAGTAHLMRYHDRLAARDSYRTTLPPPGPPRRAA"
     gene            6160..>7227
                     /locus_tag="VNZ06_11750"
     CDS             6160..>7227
                     /locus_tag="VNZ06_11750"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWW21473.1"
                     /translation="MAIILAWLLLVPGVSMAQHADLDAGSSSGPLEEVDVIANTPLPG
                     TAIDADKIPANVESIRAADLTREGSAALAGTLNRRLGGINLSDTLADPFQPDLIYRGF
                     AGSPVLGTPEGLAVYQNGVRINEAFGDAVNWDLIPDIAIDRVDVVSANPVYGLNALGG
                     AASITMKNGFSYQGAEAELSGGSFRQRSGVVQIGATEGDFGFYAAGKVLDENGWRFFA
                     HDHIRQFYGALSRHTDALSLDLSYTHDDNQLAGQGAAPVQSLALDPHNVFTGPQSNLN
                     RLNFATLNASLALPDALSLQGALYYRDYQQIVANGNTTSFTACTDPTLAGVLCQSDGV
                     TPLTNSAGQFLPDVSKGGAQVI"
BASE COUNT         1426 a         2099 c         2346 g         1356 t
ORIGIN      
        1 accacgtgca agtagtgtga acatttacga cgagcgcgga gagttgctct gggcgaccca
       61 tctgtcaatg tcgctggagc tgaccactct ggtccaggag agcattgccg acgcgatcga
      121 gaatccttcg gttacagggc gcttcaagca gatgggtgag gtgccggtct atctgttctg
      181 gctgcggcgt gaagatccgg ccaacggtcc gccgttggca tcactggtgg tgcgactgaa
      241 ggcaggcggg aacatcgagt cctgctcatt tggtttcgtg cagcagattg tgcaaccggc
      301 gcttgacgtg ctgcgccagg acctgttggc ccgtgaggag atccagcgcc tgcgcgattc
      361 gatgcaggag cagacccagg aactcaaggt gttggaggtg gtggccgggc gcggaacgga
      421 aaccggtcag ccgggcgccg aggatctgaa gtcgattctg gccaattccg ccaagcatat
      481 gaaggtaggc atggcggcct tgatcgtacc ggacaaggga ctggcgatga ccgttgccgg
      541 cgacggtggc aagctagact accgcctgct cgccaaggcg catcgtcacc tggtgtcggt
      601 cgcacgcatg cggcgcgagc cgctgatgat taacaaggtg tcgctgcccg gtcaggaaca
      661 gtccgagtac cggatcctgg cggcccccgt ttttcggccg gacggacacg ccatcggcgt
      721 gtttgcgctg tttcgcacca gcagcagccc cgagttcacc gccagtcaca gtcgcgtcat
      781 cgaactgctg gcgcgccgtc tcgccagtgt ggtggcctac gagtacgatg aactgagcgg
      841 actgctgaca cggcaggcgt tcgagcgtcg catgaagcag attcttcagg atccgaagcg
      901 agcccaatcg gagtggacgg cgctctatat cgatatcgat cacatgcacg cgatcaacga
      961 cgcgcatggg atgcacaacg gcgaccggat cctgaagcaa ctcggcgagc tgattcgccg
     1021 tcacataccc ccgggcgcac tggcgacgcg cgtgtcggcg gatcgcttcg ccatcctgtt
     1081 gccggcgagg ctggatgatt cggtcaaggt cggtgaggcg ctatgccaga acgctccaca
     1141 attgagtgct tcgctgggcg tggacaaggt gtcggtctcc gtcagtgtcg gtgtggcccg
     1201 tgtggagtca atggagcagg atttcacgca tgtgctagcc catgccgaga cggcgtgcaa
     1261 agccgccaag gcgcgcggtg cgaatcgcgt cgagagttat caggaagata acgagagcat
     1321 cgtgcgtcgc atgactgagc tgcgtctggt cgcaaacctg cagcgcgcgc tcgccgaaaa
     1381 cacactgcag ctgttcgcgc agttgattgc gccgctgggt gaccggcagc gaccaccgca
     1441 gtttgaactg ctgctgcgtc tgattgatga aaagggcaag cgcgtcggtc ccgatcagtt
     1501 catgtcggcc gcgcagcggc acgagctgat gccggcgatc gatcgctggg tgattcagac
     1561 gacgctcgaa tcgctcaagc cgcacgcatc tctgctggcg caggacctgg tagggttcag
     1621 tgtcaacgtg tccggccatg cgttgcgcga cgccagtttc gccgaggata tcgagagcgt
     1681 cattcgggcc agcggtctgg ctcccggcgg gctatgcttt gaaatcagtg aagacgcgat
     1741 gatgggtaat ctcgcgccgg ctgagaactt catgcgccga ctatccaaac tcggatgccg
     1801 gatcgcactg gatgactttg ggaccggact gagcgcgctg gcttaccttc gcaccttgcc
     1861 gattggtacg ctcaagatcg ctggcagctt cgtacgcgat gtgctggtcg actcgaaggc
     1921 tgactcgatg gtcgcgagca tcgcgcacct ggcgcactcc atggggctga ccaccgtggc
     1981 caaacaggtg gagaccgagg aggtatgcac acgggtggct tccctggggg cggactacgc
     2041 acagggattc agcattgcct caccggtacc cctgagggag atcctctcag agctgccgct
     2101 gtacaccacg ccggcgaagc cctatttggc cgtgcaaatg gctagcggga agatcggaaa
     2161 tgcctgatgt atatcgctaa gaattattga taactagatg atttaattat ataagagtta
     2221 atcacgggcc tgcactgatc cacggacaaa gcacccgcct gaccggggcc gtcgtcgtcg
     2281 ccttaagcgc tggatccagc aaccgacgcc gctgcagatg ccggatcgcg ttctggaccg
     2341 aggacttcga cagcccagtg cgtgtggcga tcatctgcag actcgcacaa acccttcgct
     2401 ggcgccgatc ggcgctgtgt cgcagaaccg ggcgctgaag ttctacaccc agcaactcgg
     2461 cttcgaggtg gcaacggacc aggcgttcga tgagaagcag cgctggatcg agctgcgcgt
     2521 tgcgcactct catacgcggg tggtcctctt cacccctgag ggtcatgagg accgcatcgg
     2581 tacgcaattc aacggatcgc tcgcttgcga tgatgtcgat gcgacctatc ggcaactgcg
     2641 tcaacgcggc gtcgagttta tcgcggagcc gcagaagcag ccgtggggca cgttcgcggt
     2701 gttcaaggat ccggacggaa accagttcgt gctgtcctcc gcctaaaaaa gggtgttgtt
     2761 gcgcgccgct gttgggggcg gtcagcgcgc gcccgccgcg tcggtcgggt actgcgggac
     2821 cctgatgaat ttggtcgggt cgtacccctg gctgtgcgca cgctccagaa ttgactggta
     2881 gagcgcatca ggcaggtcct tggtccgggc gagcacccag ccgtagctgc gcgatgggtg
     2941 cccgatcatc acccaccgat agttcggatc caggtccagg atgaagtagt tgaccgagat
     3001 caccttcagg aaccgcagat gccaggtggc gttggtgcgc tgatcagcga tcatggctct
     3061 ggcgctggtg atgcgcttca tcggcgcatc gaatgatttt ttgcggcagc tgaaccagtt
     3121 gtcgataccg ccgtccgctc gcagtgcata gctctcgacc gaatcgacac agtgtttctc
     3181 ggcaaaattt ggaatctcgg cgatgacata ccaatccccc atgtagcgcg gcagatcgac
     3241 ctgatcgacc gtgcgcaccg gcgaacgcgt gctcgcgcag gccagcagga gtatcgatag
     3301 caggggagcg gccagggctg cgcgaaacag gtgcatgcga gcattattgc agacccggag
     3361 gccggcttgc attcgtcgcg agggaggcgg aaagtcccgt ccgagccgga cgaggtggat
     3421 catggcgaaa gtcgcgaaca cgataacgcc ggaatacctg cgcaaactca ggttgtacga
     3481 gttggcgatc gcaatggttc cggggctggt acgcaagggt gcgtcggttc cctacactgc
     3541 cgtcaacggt aacatgtcgt cctacctgca ccctcgcggg gaagtggctc tgcgtttggc
     3601 cgagcaacaa cgcaaggaat tcctcaagca atacaaagcc cggctgttcg aagcctatgg
     3661 catcgtccag aaggagtacg tgacggtgcc ggaggcactg ttggccgata cgatggcgct
     3721 gggcgcgcat tttcgggcga gcctggacta tgtcggcgga cttaaagcca agccgcccaa
     3781 gaagaaagca gcgcagaaca agaccgtaaa gagcagggca ggcaacagga caaggtctgc
     3841 cgccaaaaga aaacgataga ttcctgacta cacatcacaa cgggtgagcg aatgaaaacg
     3901 agaaggatgg ttggctactg cgtagccagt ctggcgatgg gtattgcgtc gcaactggcc
     3961 actgcggcgg gtgcgccggg gacggcggtg ccgatcgcgc tgaaggcggc ccacctgttc
     4021 gactccatct ccggcaatct gatcgatcat ggcgtggtcg tgatctcggg caacaagatt
     4081 cagtcggtcg gggccgacct gaagattccc gccgatgcga aagtcatcga tcttggggat
     4141 gcgaccctgc tgcccggctt catcgatgcg cacgtgcatc tggacagcga gatgacgtcg
     4201 aactactacc tcgatgccta cgaagggatc atgcgctttc cggccgaaca ggccctgcac
     4261 ggcgcgcgca atgccaaggt gacgctcgag gcgggcgtca cgacagtgcg tgacctggga
     4321 tccactgact acatttcgct gggactgcgt aacgcgatcc gcgacggtct cattcccgga
     4381 ccgcgcatgc tgatcgccaa ctacgcgatc ggatcgaccg gaggtcatgc cgaccaggat
     4441 ccgtatccgc cgcagcgcat cgctgtcgcc gggccgattc agggtgtctg caacggtccg
     4501 gacgaatgcc gcgaagcggt gcgctatcag atcaagtatg gggcggatgt cattaagttc
     4561 atgccatcgg gcggcgtact gtcattgtcc gatcccgtgg acaatgcgca gctcacgcag
     4621 gaggagatga acgccatcgt caccgaggcg cacaactggg gacgcaaagt cgctgcgcac
     4681 tgccacggtg acaaagccgc caagatggcg atcgccgccg gtgtggactc gatcgagcac
     4741 ggcagttttc tgaaggatga cacgctgctg gagatgaaga ggaggcacgt gtacctggtc
     4801 gcgaccctgt ttgccggatt ctgggccaat ggtcatgcgg atacgtttcc cgccccgatt
     4861 gcggccaagg ctcacgccgc ggcggcgcag gcgcaattga tgtttcagca tgccgtcaag
     4921 ctgggtgtgc cgctggcgat gggaaccgac gccggagtgg agccgcacgg gctgaacgcg
     4981 cgcgagttct cattgatgac cgagaacgga ctgtctgcgg cacaggcgct gatcgcaggc
     5041 accgcgaaca gcgcggaact gttgggtctg agtgatctga ccggaacgct ggcagtcgga
     5101 aagctcgccg acatcattgc ggtcgcgggc aatccgctca cggacatgca gaccaccgag
     5161 cacgcgctgt tcgtgatgaa ggaagggagc atctacgtga gcccgacgcg ctaggcggcg
     5221 cgacggggtg ggccgggtgg cggaagagtg gtgcggtagc tgtcgcgcgc agccaggcga
     5281 tcgtggtagc gcatcagatg agcggtgcct gccagcattt ccggcccgcc gggcagcagc
     5341 cgcaagttgt gcagaatggt cacgaggttc agatccgcga agctcagcga ctcaccggcc
     5401 agatagtggt tagccgtcag cgcgccgtcc agcaccgaca gctgtagctg caacggcggt
     5461 ttcagcgcct cgatcgccgc gagatccggc gcgccgtccg cggtctgtgg cgcgatgacg
     5521 gccagcagat aactgcgaaa ggtgccgtaa agcagtgagt tgacgacgca gatccattgc
     5581 agggcgcgcg ccaactgcct gggatcggac ggaaacaggg cgggccctgt aaagacacga
     5641 tccaggtaca cggcgatcgc gaccgactcg tacagttcaa aatccccgtg acgcaggacc
     5701 ggcatcttgc cgaatgggtt gatcgcaagc acctccgggc cgccgagcag ggattgagtg
     5761 agcgtatagg ggacaccctt ttccaggcaa gccatgcaca ccgctcgggt gtacgaggag
     5821 cgcagcgagc cgatgatttc aggcttttcc ataggtcgta cacactcctc tcgtcgcgcg
     5881 ctcaaatcgt gttgtcccaa tgacctggca ggaggtgcca ctgtgagatg cgctactccc
     5941 agtgccctgg cgcccacaca taaccggctt gcggcttcgg gacggcttcg tggttgactt
     6001 gcgacagagc ctaagctcgc ccgatatcca ccgatagcct acatctctat atagaatcca
     6061 tcgtcacaga cgatatgtcg agcccgagat cacgcgcctg tatgagtagc attgggggcg
     6121 ctctcaacgc cgagcctttc gcggatggag aatggatagt tggcgatcat cttggcatgg
     6181 ctgctgttgg ttccgggtgt ctcaatggcg cagcatgcgg acctggacgc cggttcatcc
     6241 tccggaccgc tggaggaagt ggatgtcatc gccaatacgc cgctgccggg taccgcgatc
     6301 gatgctgaca agatcccagc gaatgtcgag tcgatcaggg ctgcggatct gactcgcgag
     6361 gggagtgccg cactggcggg caccctcaac cgtcgcctcg gtggcatcaa tctcagcgac
     6421 acgttggccg atccgtttca gccggatctg atctatcggg ggttcgccgg atcgccggtg
     6481 ctcggcacgc ccgagggcct ggcggtgtac cagaacggcg tgcgcatcaa tgaggcgttc
     6541 ggtgatgcgg tgaactggga cctgatcccg gatatcgcga ttgatcgcgt cgatgtggtg
     6601 agcgccaatc cggtctacgg cctgaatgcg ctgggtggcg ccgcgtcgat cacgatgaag
     6661 aatgggttca gctaccaggg agccgaggcg gaactgtcag gtggctcgtt tcgccagcgc
     6721 tccggcgtcg tacagatcgg cgcgaccgag ggagacttcg ggttttatgc ggccggaaaa
     6781 gtactggatg agaacggctg gcgttttttc gcgcacgatc acatccggca gttctatggc
     6841 gcactgagcc ggcataccga cgcgctgtcg ctcgatctgt cctacacgca cgacgacaac
     6901 caactcgccg ggcagggtgc tgccccggtt cagagcctgg cgctcgatcc gcacaacgtc
     6961 ttcaccggcc cgcagtcgaa tctcaatcgg ctcaattttg cgacgttgaa cgcgtccttg
     7021 gctctgccgg acgcgctatc gctgcagggt gcgctctact atcgcgacta tcagcagatc
     7081 gttgccaacg gtaacaccac ctcatttacc gcctgcaccg atcccacgct ggcgggagtt
     7141 ctgtgccagt ccgatggcgt gacgccgctg accaattccg ccggtcagtt tctaccggac
     7201 gtttcgaagg gtggtgcgca ggtgata
//
LOCUS       DATMRM010000391        11379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2077560, whole genome shotgun sequence.
ACCESSION   DATMRM010000391 DATMRM010000000
VERSION     DATMRM010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11379
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2077560"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VNZ06_11755"
     CDS             <1..976
                     /locus_tag="VNZ06_11755"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HWW21474.1"
                     /translation="VVSQTITEPQWHHRRHDEFPSQLVENLYWLGRYSERCEDKARLL
                     RATLAVRTNIAIWHRGREECVRQGVFSPEGEPTESLYDDGQPFGLAADLGRLGWCATQ
                     SRSLLSAEHWRSISVMQRHFQEAGNSLADPLETLDRLLVQLTALCGFALDDMTQDDGW
                     RMLMLGRRLERIQFLASLIASSLEDGTTVKQSELDWLLDIAGVSITYRTRYVSRPQLG
                     PALQLLIFEDGSPRALAYQWRKIGRTLADLAKSIGPISDPEFDEPVAQLRAVGTDGVE
                     DEGGAGAERRQQMSAALVALAAAAGRVSDRIALRYFSLVEPDVHSVAS"
     gene            973..1995
                     /locus_tag="VNZ06_11760"
     CDS             973..1995
                     /locus_tag="VNZ06_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWW21475.1"
                     /translation="MSDVCYSVRHDTLYRYEGDVAHSHQQLHLTPRDSERQHCLSHRI
                     EVIPEPSERSEYQDAFGNTVTRVELEKPHDRLEVVAEMRVRLAPLPELDLASGEPWES
                     VRDQLRYNAQSRSVSWLEAMRFRGESPYIRIKRVFSDFAQECLIPDTSVVSVAHGVMS
                     KVHSEFRYQPGATDIATPLMEVFSNRCGVCQDFAHLMIASLRSAGLAARYVSGYLYTR
                     PSQPKRPRDSKAAKPSTQPAAKASGIKEAPARQSAIAKADEAPAAMVGADASHAWVAV
                     FAPPHGWIGFDPTNNLIINQEHVALAWGRDFGDVSPLRGVILGGGSHQLSVKVAVEKT
                     SHSAAP"
     gene            2005..4842
                     /gene="glnE"
                     /locus_tag="VNZ06_11765"
     CDS             2005..4842
                     /gene="glnE"
                     /locus_tag="VNZ06_11765"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HWW21476.1"
                     /translation="MQAIEASRQRALSALTERSPTTAARLGEFPAVIRDSLAAVLGAS
                     DFVLGALARDETLISALADRADVLFAGAPMKLPEPDAASEQQFMTQLRRWRRAEFARI
                     AWRDLAGWASLDETLGDLSQAAEAALRLAHEYALRALSERYGQPRSPTASLQQLIIVA
                     MGKLGGRELNFSSDIDLVLLYPSSGETDGPRSISNEEFFTRLAQAMIRLLETPTIEGF
                     VFRADLRLRPFGDSGPVVTNAAALEDYLQLHGRDWERYAWVKARPITNESGYDELFQQ
                     CIQPFVYRRYLDFGVFESLRDMKALIEREVQRRELADNIKLGDGGIREIEFIVQSFQL
                     IRGGQDRRLQGTSLRQALARLEGSKLLPPSAVAELDAAYVFLRRLENRLQMHADQQLH
                     RLPATEDGFEILAGAMGLASVAELRGEIERQRQRVAMHFHSVVFGGDARRSAEESPAR
                     ALPLEEADREMLAVRLREMGYADGVAGARLLLDFRQLAYVRRLDAIGARRLTALLPVL
                     VTEASGAPEPAVALRRLLRVIEAIGARSAYLALLLHNSRARQRLVRLASHGDFLVDQI
                     ASHPLLLDELIDERIFEVPPDRAGLSADLEQRMAGADQSDEEQLVERLRHFQRAAIFR
                     LGVADLLARLPVMQVSDRLTDLAELIVERAIVLAWRQLRPQLGTPMCLDAGELRPVTI
                     CVIGYGKLGGMELSYSSDLDLVFLHDSSGARQETQGARVIDNQVFFVRFAQRLVHMLT
                     MHSAAGRLYEVDMRLRPSGKGGLLITHIDAFAEYQRTEAWTWEHQALLHARSVAGAGS
                     LRARFEQVRLDILAHHVRRDSLREDVRAMRNRMRNELSRAGAGEFDLKQDPGGIADIE
                     FLAQYWALWWAQKYPPVALFSDTIRQLESVASADLVPQERIDVLTGIYRLYRTHLHHR
                     ALDDKGAIVPASEFESQRHAIITIWDEVMGA"
     gene            4868..5062
                     /locus_tag="VNZ06_11770"
     CDS             4868..5062
                     /locus_tag="VNZ06_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HWW21477.1"
                     /translation="MDSRANLRQPNAQNHYTVTLKDLPLACPMPDMSLWNSHPRVYLP
                     IEATGFAKCPYCGAEYTLER"
     gene            complement(5138..5620)
                     /locus_tag="VNZ06_11775"
     CDS             complement(5138..5620)
                     /locus_tag="VNZ06_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019823277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HWW21478.1"
                     /translation="MPSFDVVSEINLHELTNAVDQAVRELEQRFDFKGSGAKFELQET
                     TVTMSAQADFQLKQMLDILKLRIAKRGIDIGCLEVKEPEVTLANAKQQVILKHGIDAD
                     TGKQVSRLIKDSKLKVQAQIQRDKVRITGKKRDDLQDAIALLKKSELGIPLQFNNFRD
                     "
     gene            complement(5634..6032)
                     /locus_tag="VNZ06_11780"
     CDS             complement(5634..6032)
                     /locus_tag="VNZ06_11780"
                     /inference="COORDINATES: protein motif:HMM:NF039364.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6165 family protein"
                     /protein_id="HWW21479.1"
                     /translation="MDTLLVPISPGELLDKITILRIKSARLNDQAKLANVKYELTLLE
                     QIWRRSLPNDPAVAADEAQLQQVNAQLWDIEDRIREHEAQQRFDTTFVELARSVYLRN
                     DERAAIKHRINRKLDSAIIEEKSYQPYRKP"
     gene            complement(6038..7111)
                     /locus_tag="VNZ06_11785"
     CDS             complement(6038..7111)
                     /locus_tag="VNZ06_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWW21480.1"
                     /translation="MLHLSERPKSLCILRLSAIGDTCHVVPLVRTLQQAWPATQLTWI
                     IGRAEARLMSLLPEIEFITIDKRDGLRAARRLRSALQGRRFDVLLHLQLSLRASLLSA
                     FVPATLRLGFDRARARELQWLFTNARIEPRTRQHVLDSFQGFLSALGLEPGHLEWNLP
                     LPEDAQRYATSLIPDSRPTLVISPCSSHAARNWRPERYAAIADYAASVYGMRVILSGG
                     PTVLERETAAAIERHAQAPLVNQTGRDTLPGLLALLSRATVLLAPDSGPAHMATMVNT
                     PVLGLWAATRLERTGPYLSRQWCVDRYEAAARKYHGRSADELPWTEKIEPADVMDLIE
                     VADVRERLDALMAARSPPPQLLT"
     gene            7110..7889
                     /locus_tag="VNZ06_11790"
     CDS             7110..7889
                     /locus_tag="VNZ06_11790"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: protein motif:HMM:NF002475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HWW21481.1"
                     /translation="MSKILIWPEGANVRQKPIRGGAMLYDASRAGNAEASWFDPIWWS
                     GRGSVREGEEGRGSTLFIEADGRDLVLRQYRRGGFAARLSRNRYLWQGGSRTRSFSEW
                     HLLYLMRHAGLPVPTPIAACYRRTGRYLYTAQILTELIPNVTSLAARLISAPVPFMMW
                     VETGRALRRFHAEGVFHADLNAHNVLLNDGPEVWLVDFDRGALRKPGLWCDANLVRLR
                     RSIDKITATLPPERFSDADWASLLSGYFAGLAQPAPELTVA"
     gene            7889..9142
                     /gene="waaA"
                     /locus_tag="VNZ06_11795"
     CDS             7889..9142
                     /gene="waaA"
                     /locus_tag="VNZ06_11795"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF004388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HWW21482.1"
                     /translation="MRRLYTLLLYLALPWVTLVVLLRGLRDREYWRGWSERFGAGPAR
                     GGGGVWVHAVSVGEVQAAVILIEALRSRDSALDVALTCATPTGRTRAATLLPGVAVSY
                     APYDLPGSVRRFLKRSRPRMLIVLETELWPNLLFEAWRAGVPTVIASARVSERSTARY
                     QRFPSLLHDALAANVWVGAQSSADAQRFAVLGVPPARRRVLGNIKFDRTFAADLPQRG
                     LGLRARYASGRPVWVAGSTHPGEEQPILLAHRQLLQQLPQALLVLAPRHPQRSDEVAD
                     GIAALGLSFERRSLSAAASTAVLLLDTLGELMDFYAACDVAFVGGSLVPIGGHNLLEP
                     AAAGVPVLTGPHQFNSPQIARAMTDAHAVTIVHGAAELAGALIRLLQDPAARTAQGMA
                     ARAVIDANRGALARMLELIEQVGAV"
     gene            complement(9143..10549)
                     /locus_tag="VNZ06_11800"
     CDS             complement(9143..10549)
                     /locus_tag="VNZ06_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HWW21483.1"
                     /translation="MKKCLWPIGVLSVVTCGNAAAVNLLDVYNRSLDSDPLWQQASFT
                     HLAVRENRTQALLNLLPIDVTANKNFIGIGGDNIRTPAFAAASLAINLFSWDNWVALK
                     AADATVAQGEANYAAAAQSLIQRVSSQYFAVLAAQDTLAATQSALQSVQIQLDQAEQR
                     YKVGLIAITDVELAQASRDSTSATVIAAKRALATQEDLLRAITNETYQGLAGPRDDMP
                     LLNPDPASEDQWVNSAMNQNANLIASRLATDIAHDNLLTAYGGHLPTVNVSVSRNWAL
                     EHGHFNGTNPAVTQNELVGSINGLPTVVDTNDVFWSLGISVPIFTGGATQSKVRQARY
                     SWQAAKAGTDFASRQTEEQTRDAYQGLISQIAQVQALKQAVESDRVALEATQAGYQVG
                     TKTVVDVLTQRAQLLQDDTNYALAKYGYLNDLVALRLAAGNLDRATIEQINGWLIEPR
                     PPPPSVPAPAPLTPPAPP"
     gene            complement(10560..10883)
                     /locus_tag="VNZ06_11805"
     CDS             complement(10560..10883)
                     /locus_tag="VNZ06_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWW21484.1"
                     /translation="MAAEEIDPTEFKRKLDAGAIVRLIDVREPWEVAIAGLAQAINIP
                     LNELPQRLSELDPKADTVVMCKSGGRSRRATAYLASRGFERVANLTGGIDAWTHDIDP
                     SLQSY"
     gene            complement(10889..>11379)
                     /locus_tag="VNZ06_11810"
     CDS             complement(10889..>11379)
                     /locus_tag="VNZ06_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWW21485.1"
                     /translation="CGKRMLRPMIAGRIVQELALRGGDEVLEVGTGSGYLSACLARLG
                     GRVRSLELHPELAGFARANLQATVPTLPCEVVVADGMQLSDEPRYDVVVLTASLPIYQ
                     SRFEQALKVGGRLFVVVGTGTLQEANLVTRLGDNEWQRQSLFETSIEALEHAPQPEAF
                     GF"
BASE COUNT         1897 a         3555 c         3767 g         2160 t
ORIGIN      
        1 cgtcgtttca cagacgatca ccgagccgca gtggcatcac cgccgacacg atgagttccc
       61 gtcgcagctg gtggaaaatc tctactggct gggtcgctac tccgagcgct gcgaagacaa
      121 ggcgcgcttg ttgcgcgcga cgctggcggt gcgaaccaac atcgcgatct ggcaccgggg
      181 gcgcgaggag tgcgtacgcc agggagtgtt cagtcccgag ggggagccca ccgagtcgct
      241 gtatgacgat gggcagccct ttggcctggc cgcggacctt ggacgtctcg gctggtgcgc
      301 gacgcagtcc cgcagtctgc tatcagctga gcattggcgc tcgatcagtg tcatgcagag
      361 gcatttccag gaggccggca actctctggc cgaccctctc gagacactgg atcgattgct
      421 ggtgcagctg accgctctgt gtggcttcgc actcgatgac atgacgcagg acgatggttg
      481 gcgaatgttg atgctcggcc gacggctgga gcgtattcag tttctcgcga gcctgatcgc
      541 ctcgagtctg gaagatggca cgacggtcaa gcagtccgaa cttgactggc tgctggacat
      601 tgcgggcgtc agcatcacct atcgcacgcg ctacgtttcc agaccgcagc tcggtccggc
      661 gctgcaactg ctgatcttcg aggacggcag cccgcgcgcg ctggcctacc agtggcgcaa
      721 gatcgggcgc acgctcgccg atctggcgaa atccatcgga ccgatcagcg accctgagtt
      781 cgatgaaccc gtggcgcagt tgcgcgcggt cggcaccgat ggggtcgagg atgagggcgg
      841 tgccggcgcc gagcgcaggc agcagatgtc cgccgcgctg gtggcgctcg ccgcggctgc
      901 gggtcgggtc tcggaccgga tcgcgctgcg ctacttctcg ctggtggaac cggacgtgca
      961 ttcggtggct tcatgagcga tgtgtgttat tcggtgcgac acgatacgct ctatcgctat
     1021 gaaggcgatg tcgcgcactc gcatcagcag ctgcacctga cgccacgcga ttccgaacgc
     1081 cagcactgtc tgtcacaccg gatcgaagtc atccccgaac cctcggagcg ctccgagtac
     1141 caggacgcat tcggcaatac ggtgacacgg gtcgagctgg agaagccaca cgaccggctt
     1201 gaggtcgtgg ccgaaatgcg tgtgcggctt gcgccgctgc cggagttgga tctggccagc
     1261 ggcgaaccct gggagagcgt gcgcgatcaa ctacgctaca acgcgcagtc acgcagcgtc
     1321 tcgtggctcg aagcgatgcg ttttcgcggc gaatcaccct acatccgcat caagcgcgtg
     1381 ttcagcgact tcgcgcagga gtgcctgatc ccggacacga gcgttgtgtc ggtcgcccac
     1441 ggcgtcatgt ccaaggtgca cagcgagttt cgttaccagc cgggcgcgac cgacatcgcg
     1501 actccgctga tggaggtgtt cagcaatcgc tgcggcgtgt gtcaagattt cgcgcatctg
     1561 atgattgcaa gcctgcgcag cgcgggtctg gcggcacgct acgtcagtgg ctatctatac
     1621 acgcgcccgt cgcaaccgaa acggcccagg gactccaaag ccgccaaacc atcgacccaa
     1681 cccgctgcca aggcgagtgg catcaaggaa gcgccggcca gacagtcagc cattgccaaa
     1741 gccgatgagg cgcccgccgc gatggtgggc gccgatgcct cgcatgcgtg ggtggcggtg
     1801 tttgctccgc cgcacgggtg gatcggcttt gatcccacca acaacctgat catcaatcag
     1861 gagcacgtgg cgcttgcatg gggtcgcgat ttcggcgacg tgtcgcctct gcgcggcgtg
     1921 atcctgggcg gcggttcaca tcagctgagc gtcaaggtgg cggtagagaa gacatcccac
     1981 agcgccgcac cgtagcattc acccatgcag gccattgagg cgtcacggca gcgcgccctg
     2041 tcggctttga cagagcgctc gccgaccacc gcagcccgac tcggggaatt ccccgcagtc
     2101 atccgtgatt cgctcgccgc ggtgctcggt gcgagcgatt tcgtgctcgg agcactggca
     2161 cgcgacgaga ccctgatcag cgcgctggct gatcgcgccg atgtgctgtt tgccggcgcg
     2221 ccgatgaaac tgcccgaacc cgacgctgca agcgaacagc agttcatgac gcagctgcgt
     2281 cgctggcggc gcgccgaatt cgcccgcatc gcctggcgtg atctggccgg ctgggccagc
     2341 ctcgacgaga cgctgggaga tctctcgcag gcggcagagg cggcattgcg tctggcgcac
     2401 gagtatgcgc tgcgcgcact gagcgagcgc tacggtcagc cgcgatcgcc gactgcgtcg
     2461 ctgcagcagc tgatcatcgt cgcgatgggc aagctaggcg gtcgcgaact caatttctca
     2521 tctgacatcg atctggtact gctctatccg tcctcgggag aaactgacgg gccgcgctcg
     2581 atcagcaacg aggaattctt cacccgcctg gcacaggcaa tgatccgctt gctcgagaca
     2641 ccgacgatcg aaggcttcgt gtttcgcgcc gatctgcggc tgcgaccctt tggcgacagc
     2701 ggtcccgtgg tcaccaatgc cgcggcgctc gaggactatc tgcagttgca cggccgtgac
     2761 tgggagcgtt atgcgtgggt gaaagcgcgc ccgatcacca acgagtccgg ctacgacgag
     2821 ctgttccagc agtgcatcca gccgttcgtt tatcgccgct atctggattt cggtgtattc
     2881 gaatcattgc gcgacatgaa ggcattgatc gaacgtgagg tccagcgccg cgagcttgcg
     2941 gacaacatca agctcggcga cggcggaatc cgcgagatcg agttcatcgt gcaatccttt
     3001 cagctcatcc gcggtggaca ggatcgccgg ctacagggca cttcgttgcg gcaggcgctg
     3061 gcgcggctgg agggttcgaa gctgctgccg ccatccgcag tcgcggaact ggatgccgcc
     3121 tatgtgttcc tgcggcgcct ggaaaaccga ttgcagatgc atgccgatca gcagctgcat
     3181 cgactgccgg cgaccgaaga tggatttgag atcctcgccg gtgccatggg cctggcttcg
     3241 gttgccgagc ttcgcggcga aatcgaacga cagcgccagc gggtggcgat gcatttccat
     3301 tcggtagtct tcggcggcga tgcccgccga tcggccgagg agtcacccgc gcgcgcgctg
     3361 ccccttgaag aagctgatcg cgagatgctg gcggtacggc tgcgggagat gggctatgcc
     3421 gatggcgtgg ccggcgcgcg gctgctgctc gatttccgcc agctggcgta tgtgcgtcgg
     3481 ctggatgcaa tcggtgccag acgcctgacc gcgctgctgc cggtgctggt caccgaagcc
     3541 tctggtgccc ctgaacctgc ggtggcgctg cgccgactgc tgcgtgtgat cgaagccatc
     3601 ggcgctcgtt cggcctatct ggcgttgctg ctgcacaatt cgcgggcccg gcagcgcctg
     3661 gtgcggctgg catcgcacgg cgattttctg gtcgatcaga tcgcctcgca cccgctgctg
     3721 ctcgacgagc tgatcgatga gcgcatcttc gaggtcccac ccgaccgcgc cgggctcagt
     3781 gcggatctcg agcagcgcat ggccggggcc gatcaatccg atgaagagca attggtggag
     3841 cggctgcgtc actttcagcg cgcggcgatc ttccgcctgg gcgtggcaga tctgttggcg
     3901 cggctacccg tgatgcaggt cagcgaccgc ctgacggatc tcgccgagct gatcgtcgag
     3961 cgcgccatcg tgctcgcgtg gcgccagctg cgaccgcagc tcgggacgcc gatgtgcctc
     4021 gacgccgggg aattgcggcc agtcactatc tgcgtcatcg gatatggcaa gctcggcgga
     4081 atggagctca gctacagctc ggacctcgac ctggtgtttc tgcacgactc ctccggcgca
     4141 cgtcaggaaa cacaaggggc gcgggtgatc gacaaccagg tgttcttcgt gcgcttcgcg
     4201 cagcgcctgg tgcacatgct gaccatgcat tcggcggccg gccgactcta cgaggtggac
     4261 atgcggctgc ggccgagcgg caagggcggc ctgttgatca cccatatcga cgctttcgcc
     4321 gagtatcagc gcaccgaggc gtggacctgg gaacatcagg cgctgctgca tgcgcgctcg
     4381 gtcgcaggcg cggggtcgct gcgcgcgcgc ttcgagcagg tgcggctgga catccttgcg
     4441 caccatgtgc gtcgcgattc gctgcgcgaa gacgtgcgtg cgatgcgcaa tcgcatgcgc
     4501 aacgagctgt cacgggcggg cgccggtgaa ttcgatctga agcaggaccc cggcggcatt
     4561 gctgacattg aatttctcgc ccagtactgg gctctatggt gggcacagaa atatccgccc
     4621 gtggcactgt tctcggacac catccgacag ctcgagtcgg tggcgtcggc ggatctggtc
     4681 cctcaggagc ggatcgacgt cctcacgggg atctaccgct tgtatcgcac ccatctgcat
     4741 caccgcgcac tggacgataa aggggcgatc gtgccggcca gcgaattcga atcgcagcgt
     4801 cacgccatta ttacaatctg ggatgaggtg atgggcgcat gaaagggctg cagctataat
     4861 cgcgcccatg gactcgcgag ccaacctgcg tcagccgaac gcgcagaatc actacaccgt
     4921 cacgctcaag gacctgccgc tggcgtgtcc gatgcccgat atgagtctct ggaactcgca
     4981 cccgcgggtc tatctgccga ttgaggccac cggttttgcc aagtgtccgt actgcggggc
     5041 ggagtacacg ctggagcgat aacatcgctc gggcacgcgg ctatccggga acattcgctg
     5101 atgcgagtgc gccagtcgat gcgccggcga cccaggccta atcgcggaag ttattgaact
     5161 gcagcggaat accgagttcc gacttcttca gcagtgcgat ggcgtcctgc agatcgtcgc
     5221 gcttcttgcc ggttatccgg accttgtcgc gctggatctg cgcctgcacc ttgagtttgg
     5281 aatccttgat gagccgtgac acctgcttgc cggtgtcggc gtcgattccg tgcttcagga
     5341 tcacctgttg cttcgcgttg gcgagcgtga cttcgggctc cttgacctcg aggcagccga
     5401 tatcgatgcc gcgcttggcg atgcgcagtt tgagaatgtc gagcatctgt ttgagctgga
     5461 agtccgcctg cgcgctcatg gtcacggtgg tctcttgcag ctcgaacttg gcgccgctgc
     5521 ccttgaagtc aaagcgctgc tcgagctcgc gtaccgcctg atccaccgcg ttggtgagct
     5581 cgtgcaggtt gatttcggag acgacgtcga aggagggcat ggcgagtcac ccttcagggc
     5641 ttgcgatagg gctgatagga tttttcttcg atgatggccg agtcgagctt gcgattgatg
     5701 cgatgtttga tcgcggcgcg ctcgtcgtta cgcaggtaca ctgagcgggc gagctcgaca
     5761 aaggtggtgt cgaagcgctg ctgcgcctcg tgctcgcgaa tgcgatcctc gatgtcccac
     5821 aattgcgcat tgacctgctg cagttgcgcc tcgtcggcgg caaccgccgg atcattcggt
     5881 agggagcgcc gccagatctg ctccagcagc gtcaattcat atttgacgtt ggcgagcttg
     5941 gcctgatcgt tcagacgcgc gctcttgatt cgaaggatcg taatcttgtc gagcagctcg
     6001 cccggagaga tcggaaccag cagagtgtcc atgaccgcta tgttaacagt tgggggggtg
     6061 gcgaccgggc agccatgagt gcgtcgaggc gttcgcgtac atcggccact tcgatcaggt
     6121 ccatgacgtc ggctggctcg atcttctcgg tccacggcag ttcgtcagca ctgcggccgt
     6181 gatacttgcg cgccgctgcc tcgtaacgat caacacacca ctggcgcgaa agatagggtc
     6241 cggtgcgctc gaggcgtgtg gccgcccaca ggccgagtac cggtgtgttg accatggttg
     6301 ccatgtgcgc tgggccggag tcgggcgcca gcagcacggt ggcgcgcgac agcaatgcca
     6361 acaggccggg tagggtgtca cgtccggtct gattgaccag gggtgcctgg gcgtgccgct
     6421 cgatggcagc ggcggtctcg cgctcgagca ccgtcggacc gccgctcagg atcacgcgca
     6481 tgccgtaaac gcttgcggcg tagtcggcga tggcggcata gcgttcgggg cgccagttgc
     6541 gcgccgcgtg actcgaacat gggctgatca cgagcgtcgg ccgggagtcc ggaatcagag
     6601 acgtggcata gcgctgcgca tcctcgggaa gcggcaggtt ccactccaga tgacctggct
     6661 ctaggcccaa cgccgacagg aaaccctgga agctgtccag cacgtgctgc cgcgtacgcg
     6721 gctcgatccg ggcattggtg aacaaccact gcagctcgcg tgcccgcgcg cgatcgaatc
     6781 ccagccgcag cgtcgctggc acgaaggcgg agagcaggct ggcccgcagc gaaagctgca
     6841 gatgcagcaa cacatcgaag cggcggccct gcagcgcgct gcgcagcctg cgcgccgcac
     6901 gcaggccgtc gcgtttgtcg atagtgataa actcgatctc aggcaacaga ctcatcagcc
     6961 gcgcctcggc gcgaccaatg atccaggtca gctgtgtcgc cggccaggcc tgttgcagcg
     7021 tccgcaccag cggcacgacg tgacaggtat cgccgatcgc cgagagccgc aggatgcaca
     7081 ggctcttggg tcgctctgag agatgcagca tgtccaaaat tttgatctgg cccgagggcg
     7141 caaacgtccg ccaaaagccc attcgcggcg gcgcgatgct atatgacgcg tccagggccg
     7201 gcaacgcgga agcctcctgg ttcgacccca tctggtggtc gggccgaggc agcgtgcgcg
     7261 aaggtgagga aggccgcggc tcgaccctgt tcatcgaggc agacggccgc gacctggtat
     7321 tgcgccaata tcggcgcggg ggattcgccg cccggctttc ccgcaatcgc tacctgtggc
     7381 agggcggtag ccgtacccgt tcattttcgg aatggcatct actctatctg atgcggcacg
     7441 ccgggttgcc ggtacccaca ccgatcgccg cctgctaccg ccggactggc cgctaccttt
     7501 acacggcgca gatcctgacc gaactgattc cgaatgtgac ttcgctggcg gcacgtctga
     7561 tcagcgcccc ggtgcccttc atgatgtggg tcgagaccgg ccgtgcgctg cgtcgttttc
     7621 acgcagaggg tgtatttcat gccgatctca acgcacacaa cgtgctgctg aatgacgggc
     7681 cggaggtgtg gctggtggat tttgaccgtg gcgccctgcg caagcccggt ttgtggtgcg
     7741 atgccaatct ggtgcggctg cgtcgctcga tcgacaagat caccgcgacg ctgccgccgg
     7801 agcgcttctc cgatgctgac tgggcatcct tgctgagcgg ctacttcgcc ggtctggcgc
     7861 aacccgcccc ggaactgaca gtcgcctagt gcgccgtctg tacacgctgt tgctgtactt
     7921 ggcgctgccg tgggtaacgc tggtggtact gcttcgcggg ctgcgcgatc gtgagtactg
     7981 gcgcggctgg agtgagcgct ttggtgcagg accggcccgc ggcggcggcg gggtgtgggt
     8041 gcatgcggtc tccgtcggag aggtgcaggc cgcggtcata ctcatcgagg cactgcgctc
     8101 gcgcgatagc gcgctggacg tggcgctcac ctgtgccacc ccgaccggac gtactcgtgc
     8161 cgccacgctg ctgccggggg tcgctgtgag ctatgccccc tatgatctgc cgggcagcgt
     8221 acgtcgcttt ctcaagcgtt cgcgtccgcg aatgctgatc gtgctggaaa ccgagctttg
     8281 gcccaatctg ctgttcgagg cgtggcgcgc cggggtgcca accgtgatcg ccagcgcacg
     8341 cgtctccgaa cgcagcacgg ctcgctatca aagattcccg tctctgctgc acgatgcgct
     8401 ggcggccaat gtctgggtag gggcgcaaag cagtgccgat gcccagcgct ttgccgtgct
     8461 cggcgtgccg ccggcgcgcc gacgcgtgct cgggaacatc aagttcgatc gcacgttcgc
     8521 agcggatctg ccgcagcgag ggttggggct gcgcgcgcgc tatgcatcag ggcgcccagt
     8581 gtgggtcgcg ggcagcacgc atcccggcga ggagcaaccg atactgctgg cgcaccggca
     8641 gctgctgcaa cagctgccgc aggcgttgct ggtgctggca ccgcgtcatc cgcagcgttc
     8701 tgacgaggtc gctgacggca ttgcggcgtt gggcttgagt ttcgagcgcc gctcgctgtc
     8761 ggcggccgcg tccactgcgg tactgctgct ggacacgctg ggcgaactca tggacttcta
     8821 cgcggcgtgc gatgtcgcct tcgtgggcgg aagtctggta ccgatcggcg gccacaacct
     8881 gctcgagccg gcggcggccg gagtgccggt tctgacgggt ccccatcagt tcaacagccc
     8941 gcagatcgcg cgcgcgatga cggatgctca cgcggtcacg atcgttcacg gcgccgccga
     9001 gctggcaggt gccctgattc gattgctgca agatccggcc gcacggacgg cgcaaggcat
     9061 ggccgcgcgt gcagtgatcg atgcgaatcg cggtgcgctg gcgcgcatgc tcgagctcat
     9121 cgagcaggtc ggagcggtct agctacggcg gcgccggcgg ggtcagcggg gcgggcgcgg
     9181 gcacggaagg cggtggcggt cgaggctcga tcagccagcc attgatctgt tcgatcgtcg
     9241 cgcgatccag gttgccggcg gccaggcgta gcgctaccag atcattcagg tagccatact
     9301 tggcgagcgc gtagttggtg tcatcctgca acagctgcgc ccgctgggtc agcacatcga
     9361 ccacggtctt ggtgccgacc tggtagccag cctgggttgc ctccagcgcc acgcggtcgg
     9421 attcgacggc ctgcttcaac gcttgcacct gcgcgatctg gctgatcaat ccctggtacg
     9481 catcacgcgt ctgttcttcg gtctggcgcg aggcgaaatc agtaccggcc ttcgcggcct
     9541 gccagctgta gcgcgcctgg cggacctttg actgggtggc cccgccggtg aaaatcggca
     9601 ccgatattcc gagcgaccag aagacatcgt tggtgtcgac cacggtgggc agaccgttga
     9661 tcgagccaac cagctcattc tgcgtgacgg ccggattggt gccgttgaag tgaccgtgct
     9721 cgagtgccca gttgcgcgac acggacacgt tgacggtcgg caggtgtccg ccataggccg
     9781 tcagcagatt gtcgtgcgcg atgtcagtgg ccagcctgct cgcaatgagg ttggcattct
     9841 gattcatcgc gctgttgacc cactgatcct cgctggccgg atccggattg agcagcggca
     9901 tgtcgtcacg cgggccagcc aggccctgat aggtttcgtt ggtgatcgcg cgcagcagat
     9961 cctcctgggt cgccagtgcg cgcttggccg cgatcacggt ggcgctggtg ctgtcgcgcg
    10021 acgcctgcgc gagttcgacg tcggtgatcg cgatcagtcc taccttgtag cgctgctcgg
    10081 cttgatcgag ttgtatctgc accgattgta acgcgctctg cgtcgccgcc agcgtgtcct
    10141 gcgccgccag cactgcaaag tattgtgagg acacgcgctg gatcaggctc tgggccgctg
    10201 ccgcatagtt ggcctcaccc tgtgccaccg tcgcgtcggc cgccttgagt gcgacccagt
    10261 tgtcccacga gaacaaattg atcgcgagcg atgcggcggc gaaagccggg gtgcgaatgt
    10321 tgtcgccgcc gatgccgatg aaattcttgt tggcggtaac gtcgatcggc agcaggttca
    10381 gcagtgcctg cgtgcgattc tcacgcacgg ccaaatgggt gaaggacgcc tgctgccaca
    10441 gggggtcact atcgagtgag cggttgtaga cgtcgagcag attgaccgct gcggcattgc
    10501 cgcatgtcac gacgctgagc acgccgatcg gccacagaca cttcttcatg agtgtcgaac
    10561 taataactct gcaggctcgg atcgatgtcg tgtgtccagg cgtcgatgcc tccggtcaga
    10621 ttggcgaccc gctcgaagcc acgcgacgcg agatacgcgg tcgcgcggcg gctgcggccg
    10681 ccggacttgc acatcacgac ggtgtcggcc ttcggatcca gctccgacag gcgctgaggc
    10741 aactcattga gaggaatatt gatcgcttgc gccaggcccg cgatggccac ctcccacggt
    10801 tcgcgtacgt cgatcaggcg cacgatcgca cccgcgtcca gcttgcgttt gaattcggta
    10861 ggatctattt cctcggcagc catagtcgtt agaagccaaa agcctccggt tgtggtgcgt
    10921 gttccagcgc ctcgatcgag gtttcaaaca gcgactggcg ctgccactca ttgtcaccaa
    10981 gcctggtcac aaggttggct tcctgcaacg tgccggtgcc gaccacgacg aacaggcgtc
    11041 caccgacctt caatgcttgc tcgaaccggc tctgatagat tggcagcgag gcggtcagca
    11101 ccaccacgtc gtagcgcggc tcgtccgata gctgcatccc atccgcgacc accacttcac
    11161 agggcagggt gggtacggtt gcctgcagat tggcgcgggc gaaaccggcc agctccgggt
    11221 gcagttcgag cgatcgtacg cgccctccca ggcgggccag gcaggcgctc aggtagcccg
    11281 atccggtgcc aacctccagc acttcgtcgc cgccccgtaa cgccagctcc tgcacaatgc
    11341 gtcccgcgat catcggccgc agcatgcgtt tgccgcacg
//
LOCUS       DATMRM010000392        12143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3304144, whole genome shotgun sequence.
ACCESSION   DATMRM010000392 DATMRM010000000
VERSION     DATMRM010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12143
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3304144"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="VNZ06_11815"
     CDS             complement(<1..1291)
                     /locus_tag="VNZ06_11815"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HWW21486.1"
                     /translation="MRLDQLVRPIVILALLALLALLAPLSARSAEAKYPDPPQVVYKD
                     LFVAVQKQALFQDEKAFPDATARSVPAGILLQYHKLHPTSPDALRGFVDAHFSLPAEA
                     SAPPSPPQQVPILRHIDSLWDTLTHTSSTAPRYSSLLSLPHPYVVPGGRFREIYYWDS
                     YFTMLGLEQSNRHDLVEDMVRDFGYLIDTYGHIPNGTRSYYLSRSQPPLFFAMVALFS
                     PKDPAAAYARYLPQLKREYAFWMDGATALQPGKAHRRVVAMPDGSILNRYWDDRNTPR
                     DESYREDVELAHQSARPAQQVYREIRAAAESGWDFGSRWFADGHTRSSIDTTEIVPID
                     LNSLIYGLENAIRSGCHRRSDQTCAREFEKRAKKRAAAIDHYLWDDSAGVYYDYRWTQ
                     SATVARLSAATLYPLFVGLASAAQAESVGTATTRDLLK"
     gene            complement(1288..2589)
                     /locus_tag="VNZ06_11820"
     CDS             complement(1288..2589)
                     /locus_tag="VNZ06_11820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HWW21487.1"
                     /translation="MTAHSAPDAQADGAPTDLGAASQDAPDLRIFVMGLFFIFGGITS
                     LNDVLIPKLKELFTLSHADAMLVQSAFFVSYFIVSLPAAAVVRRFGYLRTAVIGLLTM
                     TAGCLLFVPASSAGTFVAFLLALFVLGAGITTVQVVANPLISMLGAPATAHSRLTFAQ
                     AFNSLGTTIFPYVGSILILGSLAATDASKLTGAALSAYRTQATHAVVLAYLGLAAGCA
                     IVALLVWLRRNRLVETPLESVTFRHALNLLRDHRFGFGALCIFLYVGAEVSIGSLIVN
                     YLEQTNVLGLAAEDAGKHVPYYWGGAMFGRFIGSALLRLFSPGKVLAFAGAMNVLLLV
                     ISMTLVGPVSGWSLLAIGLFNSIMFPTIFSLASEGLGPRAAEGSGIICVAIVGGAVVP
                     RLTGLAVDWINASDLKSGLFIPAICYLIILAFGIFARRPKA"
     gene            complement(2700..3146)
                     /locus_tag="VNZ06_11825"
     CDS             complement(2700..3146)
                     /locus_tag="VNZ06_11825"
                     /inference="COORDINATES: protein motif:HMM:NF027545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4880 domain-containing protein"
                     /protein_id="HWW21488.1"
                     /translation="MSEVVRLRTRAEIDEQAASWVWRLDAGPLAADQQRAYEAWLRQG
                     PRHRRSMEELSRVWVALDQLAESGLTDPHLAVEPESSRQAFPGQRHLWWVAAAAALTA
                     AVIGVVWLQPGSESQVYSTTLVNQGQVEVRSAPPAAAIPAHTDPRR"
     gene            complement(3143..3634)
                     /locus_tag="VNZ06_11830"
     CDS             complement(3143..3634)
                     /locus_tag="VNZ06_11830"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWW21489.1"
                     /translation="MADARHDWFTTEVCRHRTALQRYLSRFASGAADIEELVQDTFVR
                     VYALDDYRSVESPKALLFRIGHKLAVERARRQPHPATDRGMDGLAPLCRRVLQLRKVF
                     KLSLEEISQVLDLSPSAIEKHVVRGLVGYRDYLRESGLLETMDPKLKPGTVCRLRDGG
                     DAE"
     gene            complement(3657..4973)
                     /locus_tag="VNZ06_11835"
     CDS             complement(3657..4973)
                     /locus_tag="VNZ06_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWW21490.1"
                     /translation="MSSSDDRPAGPPCVHAQLEVGENDEALPHMMRDFAALGDLYRVV
                     IPSRERDTWVLSDPESIKRVLVSNHRSYTIGVGLDRVKVLVGNGIIVSEGEVWRRQHR
                     MMQPMFQRAQVERFGSTIEAITKRRLADWAAKSVSGEPFNITRETSEGALEVVLRATF
                     GADYDEMSQTEGGNPFAMLSEDTSRDLRFAYRFRQLGKHIDQVIGRREASPPGDSAID
                     WLAMMMAARDRDGKPMSHRELIDEVMSLIVAGHETTAAVLNSVWYLLAQHPAVEAKLH
                     AEVDAIELPDLRLESLESLHYTHQVIAEALRLYPPVWVLSRRCIHADRLLGHQAPAGA
                     DVLMSPYVVHRHPQHWSDPEQFRPERFAPEAMADQHRYAYIPFSAGPRHCVGETFAIY
                     EMAIHLYHAARSFRLRSERTGSLEMEAKINLRVREELRMTVERRRE"
     gene            complement(5006..6418)
                     /locus_tag="VNZ06_11840"
     CDS             complement(5006..6418)
                     /locus_tag="VNZ06_11840"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWW21491.1"
                     /translation="MPQNWTSLLALGLPLLHFALAASVTVHVLMTKRNPGSSVAWIGL
                     AWLSPVIGSLLYALLGVNRVRRRARSLHEPGNPLRQPPPIGGDRSDHLAPLEFAGRRI
                     TQRCVAEGNDIRILVNGDAAYPQMLAAIDASSTSVVLSSYIFRADRAGNAFIRSLIAA
                     NQRGVEVRVLIDGYGAGYLWSRTYYELRRARVPVARFLHSAMPWRMPFLNLRSHRKIL
                     IVDGSLAFTGGLNIGAENLVSTHPRYPVLDTHFRVAGPVVSQLCGAFAELWTFTTGEA
                     LLGSTWCPLLQRAGDSIARAVTSGPDQDLEKIEFLVLSAVACAQFSIQIMTPYFLPDD
                     RIITALSLAAIRGVTIDIVLPESSNHPSVDWSTRAHIEPLLIAGCRVWTHSLPFDHSK
                     LLTVDSTWCFIGSANWDMRSFRLNFEINLEAYHSALVEQVSAIIRANQKTRLTLQDLE
                     RRSWPVRLRDSAARLMLPYL"
     gene            complement(6438..7247)
                     /locus_tag="VNZ06_11845"
     CDS             complement(6438..7247)
                     /locus_tag="VNZ06_11845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWW21492.1"
                     /translation="MLPISLVVITYNEQDAIARCLDSVDFAAEKVVVDCGSEDQTCTI
                     AAAHGARVVHQPWLGFGAQRNFADSLASHEWILSLDADEALTPELAQELKARLAALLN
                     STASAGVLYRTAWFMGRPLHWYRAMVRERKARIYHRGRARWSEARVHESLRYQGGEVV
                     FQAPLMHFLNPTLVHHELKSLRYAELKALDWRDQRHSSVPALWPLVFAFTFLKDYLLR
                     LAVLDGWRGWIVAYLAADYALYKRLRYFEMQRFAPSVELASQELKAHKLER"
     gene            complement(7234..8274)
                     /locus_tag="VNZ06_11850"
     CDS             complement(7234..8274)
                     /locus_tag="VNZ06_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWW21493.1"
                     /translation="MNARPLVVRFGALGDMVILTVAIRHLQARFGQPVDVIAAAEWTA
                     ELLGDQPGVGDIQAIGSRKRPYWLSRPQQRLVRWLRERGPGPTWLFDHDNRKIESLLR
                     RAGWTAEHWCHHEDMQGLMGSHFCDRWLRFAYRNPPILGGEDLPVGASDAHGELVVTP
                     AQRLLVARWLQQRGWPAQRLILIQVGNKRTMRRGLQRRRSNLKYWPEENWAAVLRGLR
                     AMHPAHEILLLGVPQEAALNERILGLASIPHAHNVVHELSIPKLIGLSELATGMVSVD
                     TGPAHLAAAVGCSVVTLFGSTEPFMYAPRGRNARVSCLIGARPGGRSMLYIEPSEVLT
                     AWREVVIEHATH"
     gene            complement(8271..9284)
                     /locus_tag="VNZ06_11855"
     CDS             complement(8271..9284)
                     /locus_tag="VNZ06_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21494.1"
                     /translation="MRVRLIGKANGVGLSRDISLLGAALRDSGCQVEELPCNRRERKR
                     RRSIFTQWKARIARARPHASRARCEVNIMLEHAWPQFLHEAALNVLVPNPEWFDRRDA
                     ALLARFDRIWAKTTATERIFAAQGCRVVQIGFDSEDRMTAGVQRQPRFLHLAGRSELK
                     GTTRLLEQWHRHPEWPALTVIQDEAAVTTAHRVTAGNVLLQTSFLDDAQLRTLQNSHR
                     FHLCLSEAEGWGHYIAEALSVGAVTLTCDAAPMNELVTAERGLLLAAPLAGMHNLSRV
                     ALFEPAALAAAVTQSQTMPAEHLNALGQAARQWFLDNKRDFPARVRRAMGDLETELRR
                     VRA"
     gene            complement(9289..10398)
                     /locus_tag="VNZ06_11860"
     CDS             complement(9289..10398)
                     /locus_tag="VNZ06_11860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWW21495.1"
                     /translation="MRLRISRGFAALRHAFRRRLGLVLARLLGPRHYQSVLSLDGVRS
                     VLVCRINGRMGNTLFLTPLIRHLHELLPDAVIDLAVANARAPELLGDMPGVRQVVLFP
                     HKTDRMVRRFLSALRRVRATRYDLAIDPVPESNSGRAVMTLCRARRRLGFVTNFQWAP
                     LTHAVSPPRDVMHQAAHPVYLIDRAIGREYSPGAVRLWLPLREDERYAGQQVVARAIG
                     RTDLSATRQVIGYFAHAAGYKMLGREWWLQFWNAFTALEPDAIAVECLPTAQHQRTDE
                     RFAGFHVASPRGLSAAIASMRMFVSTDTGPMHLASSTSVPTVALFRASDPMQYGPLKP
                     NDLSIDVSNVSPNEAARLCQAIWQASAGTAWPSNV"
     gene            complement(10444..11427)
                     /gene="rfaD"
                     /locus_tag="VNZ06_11865"
     CDS             complement(10444..11427)
                     /gene="rfaD"
                     /locus_tag="VNZ06_11865"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HWW21496.1"
                     /translation="MYLVTGGAGFIGSNLAAELAARGESVVVCDWMGSDERWRNLAKH
                     EIADVIAPEELTGWLRQASRKLRAVLHMGAVSTTTETDVDRIVRNNIRATFDLLEWCR
                     ESATPFIYASSAATYGDGESGFDDDFGVEPLARLRPLNAYGWSKHLVDRRVARLVEQG
                     ASLPPQWVGLKFFNVYGPNEYHKGTMRSVVARNFGSVRAGEPMRLFKSYRSQYADGGQ
                     LRDFIYVKDCVDVILWLLEHPKVSGLFNVGTGKARTWLDLAHALLRACGREPAVEFVE
                     MPPTLADKYQYFTQARMQRLRLAGYERAFTSLEDGVSEYVQRYLATADPYR"
     gene            complement(11427..11978)
                     /locus_tag="VNZ06_11870"
     CDS             complement(11427..11978)
                     /locus_tag="VNZ06_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWW21497.1"
                     /translation="MSGQVSGRAVILDRDGTMVVDRQYLSDPDGLMFLPGAAEGLRSI
                     YEQGYRLIVITNQSGVGRGMFSIQTVRQMNVRLMEMVRQAGARLEGVYYCPHRPEEHC
                     SCRKPGTQLMRDAASELGFEPRRAIVIGDKISDVEFGRQSGATTMLIAPDPASAQARS
                     AHPDYAVRDLREAATILLGLSEH"
BASE COUNT         2335 a         4046 c         3765 g         1997 t
ORIGIN      
        1 gcttgagcag gtcgcgcgtc gtcgcagtgc cgaccgattc ggcctgtgca gcggaggcca
       61 gacccacgaa cagtggatac agcgtcgccg ccgacagccg cgcaaccgtg gctgattgcg
      121 tccagcgata gtcgtagtag accccggcgc tgtcgtccca cagatagtgg tcgatcgctg
      181 ccgcacgctt cttggcgcgc ttttcgaact cgcgcgcgca cgtctgatcg gagcggcggt
      241 ggcagccgga gcggatcgcg ttctccaggc catagatcag gctgttgaga tcgatcggta
      301 cgatctcggt cgtatcgatg ctgctgcggg tatgcccgtc cgcgaaccag cgtgatccaa
      361 aatcccagcc gctctcggcc gcggcacgga tctcgcggta cacctgctgc gcgggccgcg
      421 cgctctgatg cgcgagctcg acgtcttcgc gatacgactc gtcgcgcggt gtgttgcgat
      481 catcccagta gcgattgagg atcgagccat cgggcatggc gaccacacgg cggtgcgcct
      541 tgccaggctg cagcgcggtc gccccatcca tccagaacgc atattcgcgc ttgagctgcg
      601 gcagatagcg cgcgtaagcg gcggccggat ctttcgggga aaacagtgcc accatcgcaa
      661 agaagagcgg cggctgtgag cggctcaggt aatagctgcg ggtgccgttt gggatgtgcc
      721 cataggtatc gatcagatac ccaaaatcgc gcaccatgtc ctcgaccaga tcgtgccggt
      781 tgctctgttc caggcccagc atcgtgaaat acgaatccca gtagtagatt tcccgaaacc
      841 gaccgccggg taccacgtag gggtgtggca gcgacagcag cgaggaatag cgcggcgcag
      901 tgctgctggt atgggtcagc gtgtcccaca gcgaatcgat gtgtcgcagg atcggaacct
      961 gttgtggcgg cgacggtggc gcgctcgctt ctgcaggcaa tgaaaaatgc gcatcgacaa
     1021 accctctgag cgcatccggg ctcgtcggat gcagcttgtg gtactgaagc aagatcccgg
     1081 caggaacact cctggccgtg gcgtccggaa acgccttctc atcctgaaac agcgcctgct
     1141 tctgcacggc gacgaacaga tccttataga ccacctgcgg aggatccggg tactttgcct
     1201 ctgcagaccg tgcgctcagc ggcgccagca gcgccagcag cgccagcagc gccagtatca
     1261 cgatcgggcg caccagctga tcaagtctca cgccttgggc ctgcgcgcga aaattccaaa
     1321 cgcgagaatg atcaggtagc agatagccgg gatgaacagt ccggacttca ggtcgctggc
     1381 gttgatccag tccaccgcca gaccggtcag tctcggcacc accgcgccgc cgacgatcgc
     1441 gacgcaaatg atgcctgagc cctccgcggc acgcggcccg agtccttcgc tggcgagaga
     1501 aaaaatggtc ggaaacatga tcgagttgaa cagcccgatt gcaagcagcg accagcccga
     1561 gaccggtccc acaagggtca tcgagatcac cagcaacagc acgttcatcg cgccggcaaa
     1621 ggccagcacc ttgcccgggg agaacaggcg caacaacgcg gagccgatga agcgtccgaa
     1681 catggccccg ccccagtagt acggcacgtg cttgccggca tcttcggccg cgagccccag
     1741 cacattcgtc tgttccagat agttgacgat cagcgaaccg atggagacct ccgcacccac
     1801 gtacaggaag atgcacagcg cgccgaaacc aaagcgatga tcgcgcagca gattgagagc
     1861 atgacgaaac gtcacggatt ccagcggcgt ctcgaccagt cgattgcggc gcagccacac
     1921 cagcagtgca acgatggcgc atcccgccgc cagacccaga taggccagca ccaccgcatg
     1981 ggtcgcctgg gtgcggtagg ccgacagcgc ggcccccgtg agcttggaag cgtcggtcgc
     2041 ggccagcgac cccaggatca gaatcgatcc gacgtaggga aatatcgtgg taccgagcga
     2101 gttgaaggcc tgcgcgaagg tcagccggct atgcgcggtg gctggcgcac cgagcatcga
     2161 gatcagcgga ttggctacca cctggaccgt ggtgatgcct gcacccagga caaacagcgc
     2221 cagcagaaag gcgacgaagg ttccggccga ggacgccgga acgaacagca gacatccggc
     2281 agtcattgtc agcaggccga tgacggcggt acgcaggtaa ccaaagcgtc gtaccaccgc
     2341 cgctgccggc agcgacacaa tgaaatacga cacgaagaac gccgactgca ccagcatcgc
     2401 gtccgcatgc gagagcgtga acagctcctt cagcttcgga atgaggacat cgttgagact
     2461 ggtgatgcca ccaaagatga agaacagtcc catcacgaaa attcgcaggt ccggtgcgtc
     2521 ctgagaggcc gcgcccaggt cggtaggcgc gccgtccgct tgggcgtcgg gcgcagagtg
     2581 cgctgtcatg gataccccgc ttgttggccg cacgacctta cctgatgggc cgcgcaaaat
     2641 catgacgccc ggatcctaca tatccggcct gggcgttcga ctgcgtacag cgcttgcgct
     2701 cagcggcgcg gatcggtatg cgccggtatc gcggccgcgg gcggtgccga gcgcacttcg
     2761 acctgacctt ggttgacgag cgtcgtggaa tagacctgcg actcactgcc gggctgcagc
     2821 cagacgacgc cgatcaccgc ggcggtgagc gccgcggcag ccgcgaccca ccacaggtgg
     2881 cgttggcctg ggaatgcctg ccgcgatgat tcaggctcta cggccagatg aggatccgtc
     2941 aatccggact cagccagctg atccagtgcg acccaaaccc tggacagctc ctccatcgag
     3001 cgccggtgcc gcggaccctg gcgcagccac gcttcatagg cgcgctgttg atcggctgcg
     3061 agcggtccgg cgtccaggcg ccagacccag ctcgcggcct gctcatcgat ctcggcgcgg
     3121 gtgcgcagtc ggaccacttc gctcattcgg catctccgcc gtcacgcaga cgacataccg
     3181 tgcccggttt gagcttcgga tccatggttt ccagcagccc tgactcgcgc aggtagtccc
     3241 ggtagcccac cagtcccctg accacgtgct tttcgatggc gcttggagag agatcgagca
     3301 cctgcgaaat ctcctccagc gaaagcttga acaccttccg caactgcaat acgcgccggc
     3361 acagcggggc cagcccgtcc atcccccgat cggtcgccgg atggggctga cggcgagcgc
     3421 gctcgacggc cagcttgtgg cctattcgaa acagcagggc tttgggcgat tccacgctcc
     3481 gatagtcgtc gagcgcgtag acccgaacga acgtgtcctg gaccagctcc tcaatgtctg
     3541 cggcgcccga cgcgaatctc gacaggtacc gctgcagcgc cgtgcgatgg cggcagactt
     3601 ctgtcgtgaa ccaatcgtgc cgtgcatcgg ccatcggaaa ccccctaggg gctagtctac
     3661 tctcggcggc gctccacggt catcctcagc tcctcacgca cgcgcagatt gatcttggct
     3721 tccatctcga gcgacccggt gcgctcgctt cgcaagcgaa aggagcgcgc cgcgtgatag
     3781 agatgaatcg ccatttcgta gattgcgaac gtctcgccga cgcagtgccg cgggccggcg
     3841 gaaaacggaa tgtacgcata gcgatgctga tcggccatgg cctccggcgc gaagcgctcg
     3901 gggcggaact gctccggatc gctccagtgt tgcgggtgcc ggtgtacaac gtacggactc
     3961 atcagtacgt cggcgcccgc tggcgcctga tgacccagca gtcgatcggc atggatacaa
     4021 cggcgcgaca gcacccacac cggcggatat aaccgcaacg cctcggcaat cacctgatgc
     4081 gtatagtgca gcgactcgag tgactcgagc cgcagatccg gcagctcgat cgcgtccact
     4141 tcggcgtgca gcttcgcctc cacggcagga tgttgcgcca gcaggtacca caccgagttg
     4201 agcactgcgg cggtggtttc gtggccggcg acgatcagcg acatcacttc gtcgatgagt
     4261 tcacgatgtg acatcggttt gccgtcgcga tcgcgcgccg ccatcatcat cgccagccag
     4321 tcgatcgcgc tgtcgcccgg cggtgaggct tcacgccggc cgatgacctg atcgatgtgc
     4381 ttgccgagct ggcggaaccg atatgcaaag cgcaggtcgc ggctcgtgtc ctcgctcagc
     4441 atcgcgaacg gattgccacc ttcggtctgg ctcatctcgt cgtagtcggc accgaaagtc
     4501 gcgcgcagca caacctcgag cgcaccttca ctggtttccc gggtgatgtt gaacggctcg
     4561 cccgagaccg acttggctgc ccaatcggcc aggcggcgtt tggtgattgc ctcgatggtg
     4621 ctgccgaagc gctcgacctg cgcgcgctgg aacatcggct gcatcatgcg atgctgccgc
     4681 cgccacactt cgccctcact cacgatgatg ccgtttccca ccagcacctt gacgcgatcc
     4741 agacccactc cgatcgtgta actgcggtga ttggacacca gcacgcgctt gatgctctcc
     4801 ggatcggaca gcacccaggt atcgcgttcg cgactcggaa tgacgacacg gtagagatca
     4861 ccgagggccg cgaaatcgcg catcatgtgc ggcagcgctt cgtcgttctc tcccacctcg
     4921 agctgcgcgt gcacgcacgg tgggccggcc ggcctgtcgt ccgaggaact catgtcgcaa
     4981 tataccctat ggttgctcga ggccgtcaga ggtagggcag catgagacgc gccgcgctgt
     5041 cgcgcaggcg caccggccac gaacggcgct ccaggtcctg cagcgtcagt cgggttttct
     5101 ggttggcccg aatgatggcg ctgacctgct cgaccagcgc tgagtgatac gcctcgaggt
     5161 tgatctcgaa gttcaggcgg aagctgcgca tgtcccagtt ggcgctgccg atgaagcacc
     5221 aggtgctatc gacggtcagc agcttggagt gatcgaacgg cagcgagtgc gtccagacac
     5281 gacagccggc gatcagcagc ggctcgatgt gcgcgcgcgt ggaccagtcc acgctcggat
     5341 gattgctgct ctccggcagc acgatgtcga tcgtgacgcc tcgaatggcc gccagcgaca
     5401 acgccgtgat gatgcgatcg tcgggcagaa aatacggcgt catgatctgg atcgagaact
     5461 gtgcgcaggc gaccgccgac agcaccagaa attcaatctt ttccagatcc tgatcgggcc
     5521 cagaggtcac cgcgcgcgcg atgctgtctc ccgccctctg cagcagcggg caccaggtgc
     5581 taccgagcaa ggcctcgccg gtcgtgaacg tccacagctc cgcaaacgcc ccgcacagct
     5641 gcgagaccac cggcccggca acccgaaagt gcgtgtcgag taccgggtag cgcggatgag
     5701 tgctcaccag attttcggcc ccgatgttca agccgccggt gaatgccagg ctgccatcca
     5761 cgatcagaat cttgcggtgt gagcgcagat tcaggaacgg catgcgccac ggcatggccg
     5821 agtgcagaaa gcgcgcgacc ggcacccggg cgcggcgcaa ttcatagtag gtgcgcgacc
     5881 agaggtagcc agcgccatac ccatcgatca gcacacgaac ttcgacgccg cgctggttgg
     5941 ctgcaatcag cgaacgaatg aacgcattgc cggcacggtc ggcgcgaaag atgtagctcg
     6001 acagcaccac gctggtactc gaagcatcga tcgcggcgag catttgcgga taggcagcgt
     6061 cgccattcac cagaattcgg atgtcattgc cttcagcgac acagcgctgg gtgatgcgcc
     6121 gccctgcgaa ttccagcggc gccagatgat cgctgcgatc accgccaatc ggcggcggct
     6181 gacgcagtgg attgcccggc tcgtgcagcg agcgcgcgcg tcggcgcacg cggttgacgc
     6241 cgagcaacgc atacagcagg ctgccgatca ccggcgacag ccaggccaga ccgatccacg
     6301 ccaccgatga gcccggattg cgcttggtca tcagcacatg cacggtcaca ctcgcagcca
     6361 gcgcaaaatg cagcagtggc agcccgagcg cgagcagtga ggtccagttt tgtggcatca
     6421 gaggctaggg ttccgggtca gcgctcgagc ttgtgcgcct tcagttcctg actggccagc
     6481 tcgaccgaag gcgcgaagcg ctgcatctca aagtaccgca gccgcttgta gagtgcgtag
     6541 tcggccgcga ggtacgccac aatccatccg cgccagccat ccaggaccgc caggcgcagc
     6601 agatagtcct tgagaaaagt gaatgcgaaa accaggggcc acaacgccgg caccgacgag
     6661 tgccgctgat cacgccagtc gagcgccttg agctcggcat agcgcagcga cttcaattcg
     6721 tggtggacca gtgtcggatt cagaaagtgc atcagtggcg cctggaagac gacctcgccc
     6781 ccctgatagc gcagcgattc gtgtacccgg gcctcgctcc agcgggctcg cccgcgatga
     6841 tagatacgcg ctttgcgctc acgcaccatc gcgcgatacc agtgcagcgg tcggcccatg
     6901 aaccacgctg tgcgataaag cacgccggcc gacgccgtgg agttcagcag cgccgccaat
     6961 cgggccttga gttcctgcgc cagctcgggc gtgagcgcct catccgcatc cagcgacagg
     7021 atccactcat ggctggcgag gctgtcggca aagttgcgct gtgcgccaaa ccccagccaa
     7081 ggctgatgaa cgacgcgtgc accatgcgcc gcggcgatcg tacaggtctg atcctcgctg
     7141 ccgcagtcca cgacgacctt ctcagcggcg aaatccacac tgtcgaggca gcgcgcgatc
     7201 gcgtcttgct cgttgtaggt gatgacgacc agactaatgg gtagcatgct cgatcaccac
     7261 ctcacgccag gcggtcagaa cctcggacgg ttcaatgtat agcatcgatc gccctcccgg
     7321 acgcgcgccg atcagacagc tcacccgtgc atttctgccg cgcggggcat acatgaaggg
     7381 ttcagtgcta ccaaacagcg tcacgacgct acaacccacc gcggccgcca gatgcgcggg
     7441 gccggtatcc accgagacca tgccggtagc cagctccgac agtccaatca gtttcggaat
     7501 cgacagctca tgcaccacgt tgtgagcgtg ggggatgctg gcaagcccca agatgcgctc
     7561 attcagcgcc gcctcctgag gaacacccaa tagcaggatc tcatgcgccg ggtgcatcgc
     7621 tcgcagccct cgcagcacgg ctgcccagtt ctcttccggc caatacttga gattgctgcg
     7681 ccggcgctgc agaccgcggc gcatcgtgcg cttgttgccc acctgtatca gaatcagccg
     7741 ctgcgccggc caaccgcgct gctgcagcca gcgggcaacc agcaggcgtt gcgccggggt
     7801 aaccaccagc tcaccgtgcg cgtccgaggc accgaccggc agatcctctc cgcccagaat
     7861 gggtgggttg cggtaagcaa agcgcaacca gcgatcgcag aagtgtgagc ccatgagccc
     7921 ttgcatatcc tcgtggtgac accagtgctc agccgtccag ccggcccgcc gcagcaggct
     7981 ttcgatcttg cgattgtcgt gatcgaacag ccaggtggga cccgggccac gttcgcgcag
     8041 ccaccggacc agtcgttgct gcgggcgact cagccagtac ggtcgcttgc ggctgccaat
     8101 tgcctggatg tcgccgacac ctggctgatc acccagcagc tcggccgtcc actcagcggc
     8161 cgcgatgacg tcgaccggct gaccgaagcg cgcctgcaga tgccggatcg ccacggtcag
     8221 gataaccata tcacccaatg cgccgaagcg gaccaccagc ggacgcgcat tcatgcgcgc
     8281 acgcggcgca gctcggtttc gaggtcgccc atcgcgcgcc gcacccgcgc cggaaagtcg
     8341 cgcttgttgt ccagaaacca ttggcgcgca gcctggccca gcgcattcag atgctcggcc
     8401 ggcatcgtct gcgactgcgt gacggccgcc gccagcgcgg ccggctcgaa cagcgccacc
     8461 cgggaaaggt tgtgcatgcc ggccagaggc gcggccagca gcaggccgcg ttctgccgtg
     8521 accagctcgt tcatcggcgc cgcgtcgcag gtgagcgtga ccgcgccgac actcagggcc
     8581 tcggcgatgt agtggcccca gccttcagcc tccgacaggc acagatgaaa tcgatgcgag
     8641 ttctgcagcg ttcgcagctg cgcgtcgtcc aggaagctcg tttgcagcaa cacattgccc
     8701 gcagtcaccc ggtgagcggt cgtgaccgcc gcctcatcct gaatgaccgt cagcgccggc
     8761 cactccggat gccggtgcca ctgctcaagc aagcgcgtcg tgcccttgag ctcgctgcgc
     8821 ccggccagat gcagaaagcg gggctgacgc tgcacccctg ccgtcatgcg atcttcgctg
     8881 tcgaaaccga tctggaccac acgacagccc tgcgccgcga agatccgctc ggtcgccgtg
     8941 gtcttggccc agatccggtc gaagcgcgcc aagagcgcgg catcgcggcg atcgaaccat
     9001 tccggattgg gtaccagcac gttcagcgca gcctcgtgca gaaactgtgg ccacgcgtgc
     9061 tccagcatga tgttgacctc gcaccgcgca cggctcgcgt gcgggcgcgc gcgagcaatg
     9121 cgcgccttcc actgtgtgaa gatcgagcgc cggcgcttgc gctcgcgtcg gttgcacggc
     9181 agctcctcga cctggcagcc gctgtcgcgc agcgccgcac caagcaggct gatatcgcgc
     9241 gacaggccaa caccgttcgc cttgcctatg agccggactc gcatgcgttc agacgttcga
     9301 cggccaggct gtgcccgcgc tcgcttgcca gatggcctga cacaggcgag cggcttcgtt
     9361 cggcgagacg ttgctcacgt cgatcgagag gtcattgggc ttgagcggac cgtattgcat
     9421 cggatcggac gcgcgaaaca gggcgacggt cggcaccgaa gtcgagcttg ccagatgcat
     9481 cggcccggtg tcggtggaaa cgaacatccg catcgaggca atggcagcgg acaaaccgcg
     9541 cggtgaggcg acatggaagc ccgcgaagcg ctcatcggtg cgctgatgct gcgcagtggg
     9601 caggcactcg accgcaatcg cgtccggctc gagtgcagtg aaggcgttcc agaactgcag
     9661 ccaccattcg cgtccaagca tcttgtatcc ggccgcgtgc gcgaaataac cgatgacctg
     9721 acgcgtggcc gacagatccg tgcgtccgat cgcgcgggca acgacttgct gtccggcgta
     9781 ccgctcatct tcgcgcaacg gcaaccacag acgcaccgct cccggcgagt actcccgccc
     9841 gatcgcccga tcaatcagat acaccggatg cgcggcctga tgcatcacat cgcgcggcgg
     9901 cgacaccgca tgcgtcagcg gtgcccactg aaaattggtc acgaatccga gccggcgtcg
     9961 cgcacgacac aacgtcatca cggctcgccc gctgttggat tccggaaccg gatcgatcgc
    10021 cagatcatag cgcgtggcac gcacacgccg cagcgccgac aggaaccgac gcaccatgcg
    10081 atcggtcttg tgtgggaata gcacgacctg cctcaccccc ggcatatcac ccagcagttc
    10141 cggcgcgcgc gcattcgcca ccgccagatc aatgaccgca tcgggcagca gctcatgcag
    10201 atgtcggatc agaggcgtca ggaacaaggt gttccccatg cggccgttga tgcgacacac
    10261 cagcaccgaa cgcacgccat cgagcgacag cacgctctgg tagtgacgcg gccccagcag
    10321 gcgtgccaac accagaccga gccgccgccg aaatgcgtgg cgcagggcgg cgaacccgcg
    10381 gctgatccgc agcctcacag gtgcacgtga aaccggtggc gtcagcgcct gaatggttga
    10441 ctcctagcgg taaggatccg cagtggccag atagcgctgc acgtactcgc tgacgccgtc
    10501 ttccagcgac gtgaacgcac gctcatagcc tgccaggcgc aggcgctgca tccgggcctg
    10561 ggtgaaatac tggtacttat cagcaagcgt cggtggcatc tccacgaatt cgaccgccgg
    10621 ctcgcgcccg caggcccgca gcaacgcatg cgccagatcc agccaggttc gtgccttgcc
    10681 ggtgccgaca ttgaacagcc ccgagacctt cggatgttcc agcagccaga ggatcacatc
    10741 cacgcagtcc ttgacgtaaa tgaagtcacg cagctgcccg ccgtcggcgt actgcgagcg
    10801 atagctcttg aacagccgca tcggctcacc ggcgcgcacg ctgccgaaat tgcgggccac
    10861 gacgctgcgc atcgtgcctt tgtgatactc attcggaccg tagacgttga agaatttcaa
    10921 gcccacccac tgcggcggca gcgacgcgcc ctgctctacc agacgcgcga cacgccggtc
    10981 caccaggtgc ttgctccagc cataggcatt cagcgggcgc aggcgcgcga gcggctcaac
    11041 accaaagtcg tcgtcgaagc ctgactcgcc gtcaccatag gtggcagccg aggaggcgta
    11101 gatgaatgga gtggccgact cgcggcacca ctcgagcagg tcgaacgtcg cgcggatgtt
    11161 gttgcgcacg atccggtcca cgtcggtctc cgtggtcgtg ctgacagcgc ccatgtgcag
    11221 caccgcgcgc agcttgcgcg aggcttgccg cagccagccg gtgagttcct ccggcgcgat
    11281 cacatcggca atctcgtgtt tggcgagatt gcgccagcgc tcatcgctgc ccatccagtc
    11341 gcacaccacg acactctcac cgcgcgcggc caactcggcc gccagattgg agccgatgaa
    11401 tccggcgccg cctgtcacga ggtacatcag tgctccgaca gtccgagcag aattgtcgcg
    11461 gcctcgcgca ggtctctcac cgcgtagtcg gggtgcgccg atcgcgcctg cgctgaggcc
    11521 ggatccgggg cgatcagcat cgtggtggcg ccactctggc gtccaaattc gacatcactt
    11581 attttgtctc caatcacaat ggcccgacgg ggctcgaatc cgagttcaga ggcggcatcg
    11641 cgcatcagct gggtgccggg tttacggcac gagcaatgct cctcaggacg gtgcggacag
    11701 tagtaaacgc cctcaagacg cgcccccgcc tgcctgacca tttccatcag cctcacgttc
    11761 atctgccgca ccgtctggat agagaacata ccgcgcccga cgcccgactg gttggtgatc
    11821 acgatgagac ggtacccctg ctcatagatc gaccgcaggc cttcggcggc tccgggcaga
    11881 aacatcagcc cgtccggatc gctcaggtat tgtcggtcga ccaccatggt gccatcgcgg
    11941 tcgaggatca cggcccggcc gctgacctgg ccgctcacgc tttccgggca gccgacatgc
    12001 gtgcaagggt actgctggtg ctgtgcgccg gcagcagctc cgccagcagc actttgccgc
    12061 cgcggcgctg aaccaggtca gcgccgacga cggtttcgac cgtgtagtcg gcacccttga
    12121 ccagtacatc cggcgccagc gca
//
LOCUS       DATMRM010000393         8804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1158846, whole genome shotgun sequence.
ACCESSION   DATMRM010000393 DATMRM010000000
VERSION     DATMRM010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8804
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158846"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="VNZ06_11875"
     CDS             complement(<1..1391)
                     /locus_tag="VNZ06_11875"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HWW21498.1"
                     /translation="MLASLGIDLPTAILLCLALLLALSFEFVNGFHDTANAVATVIYT
                     HSLKPAYAVVWSGSWNLIGVLTSSGAVAFGIVALLPVELVINVGSGAGFAMVFALLVS
                     AIVWNVGTWYLGLPASSSHTLIGSIVGVGLMNSLLSPDAAFGDGVNWAKVQDTVKALL
                     LSPLLGFVLAGVLLLLLKSIVRSKHLYEAPEGNKPPPWWIRLVLIGTCTGVSFAHGSN
                     DGQKGMGLIVLILVGIVPTAFALDMSASGDALAKLSAAAVSFERNLEQVVPAGTAIPE
                     TDAAHKNLTGYLRKSKASELTLPSLEAANKRVIESMTGVTLLSNVPAAQRRSVRLDIY
                     LVSETLARMAKAKQLPAGLDAKQAGDYQKRLRALTNYIPNWVKIAVALALGLGTMIGW
                     KRIVVTVGEKIGKAHLTYAQGASAELIAFGTIEAADVLGLPVSTTHILSSGIAGTMVA
                     NNSGLQKETVRNI"
     gene            complement(1565..2296)
                     /locus_tag="VNZ06_11880"
     CDS             complement(1565..2296)
                     /locus_tag="VNZ06_11880"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWW21499.1"
                     /translation="MATPHPVLTNYYPDEAHRRDYLRDLFDRTAEDYDRVERMMGFGS
                     GPWYRRRALVRAGLASGMSVLDIGTGTGLVARQAAFIVGDSALVTGVDPSAGMIDHAK
                     VPRGVRLIAGRAETIPVADASADFLSMGYALRHISDLLIAFHEFHRVLKPGGRLCLLE
                     ITRPERALGRAMLRAYMRGWVPLLSRVIARQSATPELMRYYWDTIEACTAPGVILGSL
                     SVAGFQQVHRHVELGIFSEYRARKS"
     gene            2517..3029
                     /locus_tag="VNZ06_11885"
     CDS             2517..3029
                     /locus_tag="VNZ06_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21500.1"
                     /translation="MRRERLQLVGSDRTRYYAPVAICVYLAGLCAATIVTSAFLRDHH
                     ESIAVSAAGLFGLMMSCALGAALLFTQLRELRFVSVHTHSDAQSQYQRILALARDQGW
                     RITGDEAGQRLEARTTGSVLDAGELVVVQFRPDEVLVASICDPSIGFSLVGQRRCQHN
                     RELVLHALAS"
     gene            3145..5304
                     /locus_tag="VNZ06_11890"
     CDS             3145..5304
                     /locus_tag="VNZ06_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWW21501.1"
                     /translation="MPRFRPRALPMLLWLAGASAQAALAQVSLDEVQSSTSNSPSAER
                     LETVLIEATRVGGPQVSPTGANDYAVTARDISALPAAGTSPITDVLTHMPGVALDQNQ
                     QIHIRNTEGPQFQYQINGVLIPLDVNTNPPFVSMINPMFVQQLDLLDGVLPARYSYAT
                     GGVVEIRTKDGCQQSGADISVTAGQRSSFSPSFQYATCTAGLSSYVSVLYQQSNTAFS
                     SATPEADAIHDRTQSGQAFGFFSYPLDSSMTVTLLLSGASSTNQLPNVPDLPPAFALD
                     AVPRPPNSAQIGSHLDFGDYLALVSLIGTPSSELSYQLAYTEHGISQRFRPDQVGELV
                     YQGVASSASHIDHDHTLQGDLTYSIGDHVLGSGFYAGDYLVSANDSSRVFPRDPSCAN
                     TDGCVQSSNVPVTVINDAEASNGLLGVYLNDLWQIAPQLRANLGLRWDHLNGFTQRGQ
                     FDPTINFIYTPSHDTTIHAGFARYFQVPSFLGISPTAQTAFVNTTAAGPPGVAAPDTE
                     DDYEWDVGIAQQLTGRFTMSVDSFFERTHRYLDTGQFSDVPIFAPFNYHWGYIWGTQL
                     ATKYRGDHLSAYGNLTIGLNRQRAVLTGQFNFDPVELAYIDSHSITLDHQPLYGASGG
                     ASYQWGSYALNLDGLHSSGLRGGFADLEPLPSVTQFNLSADYSITLPGIGVLTNRLGL
                     LNLLDRVNLIRPAQGIGIFQSAYGPRFTLFDSISVHF"
     gene            5414..5764
                     /locus_tag="VNZ06_11895"
     CDS             5414..5764
                     /locus_tag="VNZ06_11895"
                     /inference="COORDINATES: protein motif:HMM:NF016244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HWW21502.1"
                     /translation="MATSDAVDWDAITRDPRFQSLHKRKSAFLWGLMAVSVIYYFLLP
                     IGAAYFQDLFKIRVWGVVNVGLLFALSEFAVAWIVAWIYSRKASRDFDQLAAQICAEC
                     THTPPLQAAAGGLP"
     gene            5761..7578
                     /locus_tag="VNZ06_11900"
     CDS             5761..7578
                     /locus_tag="VNZ06_11900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HWW21503.1"
                     /translation="MSKQTITFLVFAVVVAITMYVTYVAAKRVRSVSDFYTAGGGVSG
                     WQNGWAIAGDYLSAASFLGIAGLISLYGYDGFMYSVGWLVAYVTVLLLIAEPCRNIGR
                     YTLSDILAYRNDPTKVRTVGAISTITVSTFYLTAQMVGAGVLVKTLIGISYETSVACV
                     GVLMLLYVLFGGMVATTWVQIIKAVLLVTASIIIVLLVWTPYGFSLPAFLKDVVGDAK
                     VQARVAALLGDASKNMSASELGQRFLEPGLYFKAPIDQISLGMALVFGTAGLPHILMR
                     FFTVPTAQQARKSVVWAMVVIGGFYVLTLFLGFGAAHHVGPAAIAAVDAGGNMAGPLL
                     AQFIGGGPDSMLGNFMLAFVSAVAFATIVAVVSGLVLASASAMAHDLYVGVIRHGKSV
                     TPQGQVTAARIATLIVGALAIGIGLAAKGQNVAALVGLAFAVAASANFPCVLLTLYWK
                     RCNTGGIVTGMVVGTIAAVGLTLVSPNLTYPKAVKAAAHKVLDGEAARRAAVSAALGA
                     SDEKAASQAKKELAALDKNVAKAKDDIAKWGDTNTSFMGLQEPMTQLKNPGIISIPLG
                     FLAVIFGSLLFRDKRAEDMWNEVYVRQNTGLLVAPPTSH"
     gene            complement(7603..8370)
                     /locus_tag="VNZ06_11905"
     CDS             complement(7603..8370)
                     /locus_tag="VNZ06_11905"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWW21504.1"
                     /translation="MSWNPEQYLRFSQPRLRPAIDLLGRIALDSPQRIYDLGCGAGNV
                     TALLRARWPAAALTGVDSSAAMLEKAATALPHVSWLQQSLDQWRAEAAADLIFSNAAL
                     HWLGDHEHLFPRLLTSLRAGGVLAVQMPRNFAAPSHTLIADTVRAGPWRSQLESLLGP
                     PPVSEPAFYYSVLAPLSQTLDIWETEYLQVLSGEDPVKEWTKGTALIPYLQRLSPAQH
                     RPFESDYAQRLRTAYPRRADGTTLFPFKRLFILLQRK"
     gene            complement(8367..>8804)
                     /locus_tag="VNZ06_11910"
     CDS             complement(8367..>8804)
                     /locus_tag="VNZ06_11910"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWW21505.1"
                     /translation="ELGIVIGTRCKEVSAEQAGEYIFGYTCVNDVTAADHFKETADFE
                     QWTRAKSYDTFGVLGPVIATGLDVSQLNVVTRLDGVERQNYPVADMIFSPAQIVSRIS
                     QDMSLLSGDVIACGTSLGVGSMRPGARVEITIAGIGALTNVVG"
BASE COUNT         1635 a         2821 c         2662 g         1686 t
ORIGIN      
        1 atgatattgc gcaccgtctc tttctgcagc ccggagttgt tcgcaaccat cgtgccggcg
       61 atgccggagg acagaatatg cgtcgtactg accggtagcc ctaagacatc ggcggcctcg
      121 atggtgccga aggcgatcag ctcggcactc gcgccctgcg cgtaggtcag gtgcgccttg
      181 ccgatctttt caccgaccgt cacgacgatc cgcttccagc caatcatggt gcccagaccc
      241 agcgccagag cgaccgcgat cttgacccag ttgggaatgt agttcgtcag tgcgcgcagc
      301 cgcttctgat agtcgccggc ctgcttcgcg tcgagccccg cgggtagctg cttggccttg
      361 gccatgcggg ccagcgtctc ggacaccaga tagatgtcca ggcgcactga gcggcgttgg
      421 gccgcgggga cattcgacag gagggtgacg ccggtcatgc tctcgatcac gcgcttgttg
      481 gcggcctcga gtgacggcag cgtcagctcc gaggccttgc tcttgcgcag atacccggtc
      541 agattcttgt gggcagcatc cgtttcaggg atcgccgtcc cggccgggac cacctgctcg
      601 agattgcgtt cgaagctgac cgccgcggct gacagcttcg ccagcgcgtc gcccgaggcg
      661 ctcatatcca gcgcaaacgc ggtcgggacg atgccgacca ggatcagcac gatcaagccc
      721 atgcctttct ggccatcatt cgaaccgtgc gcgaagctga caccggtgca ggtaccaatc
      781 agcaccaggc ggatccacca tggcgggggc ttgttgcctt ccggggcctc atacaagtgc
      841 ttggagcgca cgatcgactt cagcagcagc aacaacactc cggccagcac aaagccgagc
      901 agcggcgaga gcagcagtgc cttgacggtg tcctgcacct tggcccagtt gacgccatcg
      961 ccgaaggccg cgtcagggct cagcagggag ttcatgagac caacgccgac gatcgagccg
     1021 atcagtgtgt gtgaactcga ggccggcagg cccagatacc aggtgcccac gttccatacg
     1081 attgccgaga ccagcagcgc gaacaccatt gcaaagccgg cacccgagcc gacgttgatg
     1141 accagttcga ccggcagcag agccacgatg ccaaacgcga ccgcacccga ggaggtcagc
     1201 acaccgatca ggttccagct tcctgaccag accacggcat aagccggttt tagggaatga
     1261 gtgtagataa ccgtcgcaac tgcgttcgcc gtatcgtgaa atccgttcac gaactcaaag
     1321 ctcagcgcga gcagcaatgc cagacacagc agaattgcgg tcggcaagtc gatgcctagc
     1381 gatgcaagca cgggatccct ctctattgat gttataggtt cgaaagaaag tgcgaaggca
     1441 gactatgacc gtcctgtgac gctttcatga cgcgccttgg tacgcgagat taatttcgtc
     1501 agcaagcacc tgtccctgcg gacctacccc ttgggtattc tctgacagct atttcgaagg
     1561 gtcttcagct ctttcgtgca cgatattcgg agaaaattcc caactcgacg tggcgatgga
     1621 cctgctgaaa tcccgccacc gacaatgagc ccagaatcac gccaggtgcg gtgcaggcct
     1681 ctatcgtgtc ccagtagtac cgcatcagct caggcgtagc agactgacgc gcgatcacgc
     1741 gcgacagcag cggcacccag ccgcgcatgt acgctctgag catcgcgcgc cccagcgctc
     1801 gctcaggccg cgtgatctcg agcagacaca gtcgcccgcc gggcttcagg acccgatgaa
     1861 actcatggaa ggcgatcagc agatcgctga tgtgccggag cgcgtacccc atactcagaa
     1921 aatctgctga cgcgtcggcg accggaatgg tttccgcgcg gccggcgatc aggcgcacgc
     1981 cccgaggaac cttggcgtga tcgatcatgc cggcactcgg atccacaccg gtgaccagtg
     2041 ctgaatcgcc gacaatgaac gccgcttggc gcgccacgag cccggtgccg gtgccaatgt
     2101 cgagcaccga catgcccgag gccagtcccg cacgcaccag cgcccggcgc cgataccatg
     2161 gccccgagcc aaaccccatc atgcgctcta cccgatcgta gtcctcagcg gttcgatcga
     2221 agaggtcccg aagatagtcg cgccgatgcg cttcatcagg gtaatagttg gtgagaaccg
     2281 gatgcggtgt cgccatggtg cgcaggctac gccatgagcg gtgccgaaag ctatccgatg
     2341 cccgtcacgc catccgtgag gcatgcaacg cagctgcaac tccacgcaca cctcgctttc
     2401 accagcgcga cacagttgca aatcgcttct ggggctacat aaagaagaaa ggcgtgcgag
     2461 cttgtgtgaa cttcatcgct ggcgcttcgc ggggaatctg ttatctattg aatgccatgc
     2521 gtcgtgaacg acttcaactg gtcgggagcg acaggacgcg ctactacgcg cccgtcgcga
     2581 tctgtgtcta tcttgccggc ctgtgtgccg cgaccatcgt cacgtcggcc ttcctccgcg
     2641 atcaccacga gagcattgca gtcagcgcgg cgggcctgtt tggcctgatg atgtcgtgcg
     2701 cgctgggtgc cgcgctgctg ttcacgcagc tgcgggagct gcgcttcgta tccgttcaca
     2761 cccattcgga tgcgcagtcg cagtatcagc gcattctggc gctggcccgc gatcagggct
     2821 ggcggatcac cggcgatgag gccgggcagc gcctggaagc gcgcaccacc ggatcggtgc
     2881 tcgacgccgg agaactcgtg gtggtgcagt tccgaccgga cgaagtgctg gtcgccagca
     2941 tctgcgatcc gagcattggt ttctcgctcg tgggccagcg ccgctgccag cacaaccgcg
     3001 agctggttct gcacgctctc gccagctagg gcgctgcacg ccgtctttct cgctcgtctc
     3061 ttgacggcgc caattgggcc gtcctaatat ggctaatacg aacgattatc gttagcattc
     3121 gtgttaacag aacgaggagc ggcagtgcct cgattccgcc cccgggcctt gccgatgttg
     3181 ctgtggcttg ccggcgcgag tgctcaggcg gccctcgcgc aggtctccct cgacgaggtg
     3241 caatcctcca cctccaacag ccccagcgcc gagcgtctgg aaacggtcct catcgaggcc
     3301 acgcgggtcg gaggtccgca agtctcccct accggcgcga acgactacgc ggtgacggcg
     3361 cgcgatattt ccgcgttgcc cgccgcgggt acctccccga tcaccgatgt cctgacgcat
     3421 atgccgggtg tcgcgctcga ccagaaccag cagatccaca tccgcaacac cgagggaccg
     3481 cagttccagt accagatcaa cggcgtgctg attcccctcg acgtcaacac caatccaccg
     3541 ttcgtctcga tgatcaatcc gatgttcgta cagcagctgg acctgctgga tggggtgctg
     3601 cccgcgcgtt acagctacgc caccggcggt gtggtcgaga tccgcaccaa ggatggctgc
     3661 cagcagtcgg gcgcggatat cagtgtcacc gccggccagc gcagcagctt cagcccgagc
     3721 tttcaatacg cgacctgtac cgcaggcctg agttcctacg tcagtgtgct ctatcagcag
     3781 agcaacaccg cgttcagttc cgccacaccc gaagcggatg caatacacga tcgcacccag
     3841 tcagggcagg cgttcggctt cttttcctac ccgctcgaca gctcgatgac agtcacactg
     3901 ttgctgtcgg gcgccagcag caccaatcaa ctgccgaacg tcccggatct gccgccggcg
     3961 tttgcgctgg acgcggttcc tcgtccgccg aactccgcgc agatcggttc gcacctggat
     4021 ttcggtgact acctggcgct ggtttcgctg atcggcacgc cgagcagcga actgagctat
     4081 cagctggcgt acaccgaaca cggcatctca cagcgctttc gcccggatca ggtcggcgag
     4141 ctcgtctatc agggggtcgc ctccagcgcc agtcacatcg accacgatca cacactgcag
     4201 ggggatctca cctacagcat cggcgatcat gtactcggca gcggttttta tgccggggat
     4261 tatctggtga gcgccaacga ttcgtcacgg gtgtttccgc gggatccctc gtgcgccaac
     4321 accgatggct gcgtacagag cagcaacgtg ccggtcactg tgatcaacga cgccgaagcc
     4381 agcaacgggc tgctcggagt ctacctcaac gatctgtggc agattgcacc gcagctgcgt
     4441 gcgaacctcg ggctgcgctg ggatcatctg aacggtttca cgcagcgcgg ccagttcgat
     4501 ccgacgatca atttcatcta tacgcccagt cacgacacca cgattcacgc cggattcgcg
     4561 cgctactttc aggtgccgtc gttcctcggc atctcgccca ccgcccagac cgcattcgtc
     4621 aatacgacgg ccgcgggccc gccaggtgtt gcggcaccgg acacggagga tgactacgaa
     4681 tgggacgtcg gtatcgcgca gcagctcacc ggccgcttca cgatgtcggt ggacagcttc
     4741 ttcgagcgaa cgcatcgcta tctggacacc ggtcagttca gcgacgtccc gatcttcgcg
     4801 cccttcaact accactgggg ctacatctgg ggcacgcagc tcgccaccaa atatcgcggc
     4861 gatcacctca gcgcctacgg taacctcacg atcggactga atcgccagcg cgcagtgctg
     4921 accgggcagt tcaacttcga tccggttgaa ctcgcctaca tcgatagcca ctcaatcacg
     4981 ctcgatcatc agccgctgta tggcgcctcc ggtggcgcga gctatcagtg gggctcatac
     5041 gcactgaacc tcgacgggct ccatagcagt ggtctgcgag gcggctttgc cgacctggag
     5101 cctctgccct cggtcacgca gttcaacctc agtgccgact actcgatcac gctccccggc
     5161 atcggcgtgc tgaccaatcg gctcggactg ctgaacctgc tcgaccgtgt caatctgatc
     5221 cgtccggccc aaggcatagg tatatttcaa tccgcttacg gcccgcgttt cacgcttttc
     5281 gacagtattt ctgtccactt ctgatatgcc ttgggggggc catgacaccg atttgccggc
     5341 gggcgggagg tgcgctacct tgcacacggg cctagggacg aatgcccact ataaaaacca
     5401 aagggggtct gtcatggcaa catccgatgc cgtggattgg gacgcgatca ctcgcgaccc
     5461 gcgcttccag agtcttcaca agcgcaagag cgcgttcctg tggggactga tggcagtgtc
     5521 ggtgatctac tatttcctgc tcccgattgg cgcggcctat ttccaggatc tgttcaagat
     5581 ccgggtctgg ggcgtagtca atgtaggact gctgtttgcg ctgagtgaat tcgcggtcgc
     5641 gtggatcgtg gcctggatct actcgcgcaa ggcgagccgc gacttcgatc agctcgccgc
     5701 acagatctgt gcagagtgca cccacacgcc cccgctgcag gctgccgccg gaggtctgcc
     5761 gtgagtaagc agactattac cttcctggta ttcgccgtgg tcgtagccat cacgatgtac
     5821 gtcacctacg tggccgccaa gcgagtgcgc tcggtgtcgg acttctacac cgccggtggc
     5881 ggtgtcagcg gttggcagaa cggctgggcg atcgccggcg actacctgtc ggcggcatcg
     5941 ttcttaggca tcgcgggatt gatctcgctg tatggctatg acggcttcat gtactcggta
     6001 ggctggctgg tggcatatgt caccgtgctg ctgctgatcg ccgagccctg ccgcaacatc
     6061 ggccgttaca cgctgtcgga tatcctggcg taccgcaacg atccgaccaa agtccggacg
     6121 gtcggtgcga tttcaacgat caccgtctcg acgttctacc tcaccgcgca gatggtgggt
     6181 gccggtgtgt tggtcaaaac cctgatcggc atcagctatg aaacatccgt agcgtgcgta
     6241 ggcgtactga tgctgctgta cgtgctgttc ggcggcatgg tcgcgaccac ctgggtgcag
     6301 atcatcaagg cggtcctgct ggtcaccgca tcgatcatca tcgtgctgct ggtgtggacg
     6361 ccctatggat tctcgctgcc ggccttcctc aaggatgtcg tgggggatgc caaggtgcag
     6421 gcgcgtgtgg ccgccttgct gggcgatgcc tcgaagaaca tgtccgcgtc cgaactgggc
     6481 cagcgcttcc tcgagcccgg tctgtatttc aaggcaccga tcgatcagat cagcctcggc
     6541 atggcattgg tattcggtac tgccggattg cctcacatcc tgatgcgctt ctttacggtg
     6601 ccgacggcac agcaggcgcg caagtcggtc gtgtgggcga tggtcgtcat cggcggtttc
     6661 tatgtgctga cgctgttctt ggggttcggc gcggcacatc acgtcgggcc cgctgccatt
     6721 gcagcggtgg acgccggcgg aaacatggcc ggcccgctgc tcgcgcagtt cataggcggc
     6781 gggccggact cgatgttggg caacttcatg ctggcattcg tgtcagcggt cgccttcgcc
     6841 accatcgtgg cggtggtctc gggcctggtg ctggcctcgg cttcggcgat ggcgcatgac
     6901 ctctacgtcg gcgtgattcg tcacggcaag tccgtcactc cccagggtca ggtcaccgcg
     6961 gcacgcatcg cgacactgat cgtcggtgcc ctcgccatcg gcatcggact ggccgccaag
     7021 ggccagaacg ttgccgcact ggtgggactg gcctttgccg tggccgccag cgcgaacttc
     7081 ccgtgcgtac tgctgacgtt gtactggaag cgctgtaaca ccggcggcat cgtcaccggc
     7141 atggtcgtcg gaaccatcgc tgcggttggg ttgacgctgg tatcaccgaa cctcacctac
     7201 cccaaggccg tcaaggccgc ggcccacaag gtactggatg gggaagcggc caggcgtgcg
     7261 gcggtgagcg cggccctggg cgcctcggat gaaaaggccg cctcgcaggc gaagaaggag
     7321 cttgccgcgc tcgacaagaa tgtcgcgaag gccaaggacg acatcgccaa gtggggcgat
     7381 accaatacgt ccttcatggg gctgcaagag ccgatgaccc agctcaagaa tccgggcatc
     7441 atttcgattc cgctcggatt cctcgcggtg atcttcggct cgctgctgtt ccgcgacaag
     7501 cgcgcagagg acatgtggaa tgaagtctac gtgcggcaga acaccggact gctggtagcg
     7561 cctccgacca gccactagtt gacgcggaag attttagcga cgctacttcc gctgcagcag
     7621 aatgaacagg cgcttgaatg gaaacagcgt cgtgccgtcg gcgcggcgcg gataggcagt
     7681 gcgcaggcgc tgcgcgtagt cgctctcgaa cggcctgtgc tgtgccggac tcaggcgctg
     7741 cagatacgga atcagcgccg tacccttggt ccattccttg accgggtcct caccgctcaa
     7801 tacctgcagg tattcggttt cccagatatc gagcgtctgc gacaacggcg ccagcacgga
     7861 ataatagaac gccggttccg acaccggcgg cggtccgagc agcgattcga gttggctgcg
     7921 ccagggaccg gctcgcaccg tatcggcgat caaggtgtgc gacggcgcgg cgaaattgcg
     7981 cggcatctgt accgccagca cgcctccggc tctgagtgag gtcagcagtc gcggaaacag
     8041 atgctcatga tcgcccagcc agtgcagcgc ggcgttggaa aagatcagat ccgccgctgc
     8101 ttcggcgcgc cactgatcca gcgattgctg cagccacgat acatgcggca gcgctgtcgc
     8161 agccttttcc agcatcgccg ctgagctgtc cacaccggtg agggccgccg ccggccagcg
     8221 agcccggagc aatgcggtga cgttaccggc gccgcatcca agatcgtaga tgcgttgcgg
     8281 agaatcgagc gcgatgcggc cgagcagatc gatcgcaggt cgcagtctcg gttgtgagaa
     8341 tctcaggtac tgctcggggt tccagctcat ccaaccacat tggtcagggc accaattccc
     8401 gcaatggtga tttcgacccg cgcaccgggc ctcatcgagc ccacgccaag cgaggtgccg
     8461 caggcgatca catctcccga cagcagcgac atgtcctgcg agatgcggct gacgatctgc
     8521 gccggcgaaa aaatcatatc ggcgaccgga tagttctgtc gctcaacgcc atcgagccgg
     8581 gtcacgacgt tcagctgcga gacatccaac ccggtcgcga tcaccggccc cagcaccccg
     8641 aaggtgtcgt agctcttggc gcgcgtccat tgctcgaaat ccgccgtctc cttgaaatga
     8701 tcggcagccg tgacgtcgtt gacgcaggtg tatccgaaga tgtactcgcc ggcctgctcg
     8761 gcgctgactt ccttgcatcg cgtgccgatg acgatgccga gctc
//
LOCUS       DATMRM010000394         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4585802, whole genome shotgun sequence.
ACCESSION   DATMRM010000394 DATMRM010000000
VERSION     DATMRM010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4585802"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2443
                     /locus_tag="VNZ06_11915"
     CDS             <1..>2443
                     /locus_tag="VNZ06_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW21506.1"
                     /translation="PAVVSVTALRDAAGAIIGYLLIGTDNTARKQIEAEQTQLAQRLR
                     DHQFYTRSLFESNIDALMTTDAPGIITDVNKQMEALTGCTRDELIGAPFKTFFTDPQR
                     AEAGIKLALSKRKVVDYELTARDRDGKETVVSYNATTFYDRDRRLQGVFAAVREITER
                     KQYERSLREATQKAEQANRAKSEFLANMSHEIRTPLNAVIGLGYLLEHTRLSEDQRQL
                     LTKIQFGGRALLGVINNVLDLSKIEAGEMSLEDEPFDLQQLVRDLSQMLAPQAVAKGI
                     ELIVQAAPTLPRMVNGDASRLRQILTNLLGNSIKFTTAGHVELKVFCTEQGSDRIRLS
                     CTVQDTGIGIEPAALEGLFRPFTQADASTTRRFGGTGLGLSIARRFVELMGGEIGVIS
                     TVAVGSTFWIEIPLRIAHGTDDTLSADGARGLRILIADYNGDALAGPAAMVRALGWSP
                     QLVETGEQILEVMTNTEPNAWPDVLILNLHLLDTPARQLIARLEKECTQGELPPVIVV
                     ADVAPHEPLKRTTDVLLVRPVASSTLFNAVNAAVSRRHDGHERIFQSINFDLLHAQWL
                     AGVRVLVVDDSDINLEVARGILEKQGATVTTCTDGAAALEHVRANHEQLDVVLMDVQM
                     PVLDGNEATRRIRGERQLQKLPIVALTAGALVGERQRSLEAGMNDFVSKPFDPEALIR
                     KVRRLVEQARGQPIPIVVLDTKSVCHAVDGPLMSSIDAGVVKQMFGDDLSLFKSLLAR
                     MLRDYADLALPIRVLPDDQAIRSQLQGRTHKLKGSAGMIGATKVMLLAGAAEEALQEC
                     RPVDIVEGILRQLSSA"
BASE COUNT          494 a          738 c          772 g          439 t
ORIGIN      
        1 ccccgccgtc gtgtcggtta ccgcactgcg cgatgcggca ggtgcgatca tcggctacct
       61 tctgatcggt accgataaca ccgcccgcaa gcagatcgag gcggagcaga cgcagctggc
      121 gcaacgcctt cgcgaccacc agttctacac ccgctctctg ttcgaatcaa acatcgacgc
      181 attgatgact accgatgcgc ccggcatcat cactgacgtc aacaaacaaa tggaggcgct
      241 caccggctgc acgcgcgacg agttgatcgg cgcgccgttc aaaacgttct tcaccgaccc
      301 ccagcgcgcc gaagcgggta tcaaactggc gctgagcaaa agaaaggttg tcgactacga
      361 gctgaccgca cgcgaccggg acggcaagga gaccgtcgtg tcctacaacg cgactacctt
      421 ctacgatcgc gaccggagac tgcagggcgt gttcgctgca gtgcgtgaaa tcacggagcg
      481 taagcaatac gagcgatcgc tgcgtgaggc gacgcaaaag gctgagcagg cgaaccgggc
      541 gaagagcgag ttcctggcca acatgagcca cgaaatccgc acccctttga acgccgtcat
      601 cggactgggg tatctgctcg aacacaccag gttgagtgaa gaccaacgcc agctactgac
      661 caagatccag tttgggggcc gcgccctgct gggcgtcatc aacaatgtgc tggatctgtc
      721 aaagatcgag gcgggcgaga tgtcgctcga ggacgagccc ttcgatttgc agcagctcgt
      781 gcgggacctc agccagatgc tggccccgca ggcggtggca aaggggatcg agttgatcgt
      841 tcaggctgca cccaccttgc cgcgcatggt caacggcgat gcgtcgcgcc tgcgccagat
      901 cctcaccaac ctgctgggca attcgatcaa gttcaccacg gccggccacg tggagctgaa
      961 ggtattctgc accgagcaag gatccgatcg gatccgtctg agctgcacgg tgcaggatac
     1021 cggcataggc atcgagcctg cggcgctcga gggtctgttc aggccattca ctcaagccga
     1081 tgcgtccacg acccgtcgct ttggcggtac cgggctgggt ctgtctatcg cgcgccgctt
     1141 tgttgaattg atgggtggcg aaattggcgt catcagcacc gtagcagtgg gcagcacttt
     1201 ctggatcgag atacccctgc gaatcgcgca cggcaccgat gacacgctca gcgcagatgg
     1261 agcgcgtggc ctgcgaatcc tcattgctga ttacaacggg gatgcgctgg cgggccccgc
     1321 cgccatggtg cgtgcgcttg gctggagccc gcaactggtc gaaaccggcg aacagatact
     1381 cgaagtcatg accaacaccg agccgaacgc atggccggat gtgctcatcc tcaatctgca
     1441 cctgctcgat acacctgcac gtcagctgat tgcacgcctg gagaaggaat gcacccaggg
     1501 cgagttaccg ccggttatcg tcgtcgccga tgttgcgccg cacgaaccgc tcaagcggac
     1561 cacggacgtt ctgctggtgc gtccggtagc gagttcaacg ctgttcaatg cggttaatgc
     1621 cgccgtctcg aggcggcatg acggtcatga acggatattc caatcgatca attttgatct
     1681 gttgcatgcg caatggctcg ccggcgtgcg cgtgctggtg gtcgatgaca gcgacatcaa
     1741 tctggaggtc gcgcggggta tcttggagaa gcagggggca accgtcacaa cctgcaccga
     1801 cggcgcggcg gccctggagc atgtgcgcgc caatcacgag cagctcgatg tcgtgctcat
     1861 ggatgtgcag atgccggtcc tcgatggcaa cgaagcgacg cggcgcatcc ggggcgaacg
     1921 gcaactgcag aagctgccca tcgtggcgct gacggcgggc gcactggttg gtgagcggca
     1981 acgctcgctc gaggcgggca tgaacgattt cgtcagcaag ccgtttgatc ccgaggcact
     2041 tatccgcaag gtgcggcgcc tggtggaaca ggcacgcggc cagcctatac cgattgtggt
     2101 cctcgacacg aaatccgtgt gtcacgccgt tgacgggccg ctcatgtctt ccatcgatgc
     2161 cggtgtcgtt aagcagatgt tcggagatga cctgtcgctg ttcaaatcat tgctggctcg
     2221 catgctgcgg gactatgcgg acctggcgct gccgatccgc gttttgcccg atgatcaggc
     2281 catccgcagc cagctgcagg ggcgcaccca caagctaaag ggcagcgcgg gaatgatcgg
     2341 cgcgaccaaa gtcatgctgc tcgcgggcgc ggccgaggaa gcactccagg agtgtcggcc
     2401 ggtcgacatc gtggagggga tactcaggca actgtcgtcg gcg
//
LOCUS       DATMRM010000395        11185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2398489, whole genome shotgun sequence.
ACCESSION   DATMRM010000395 DATMRM010000000
VERSION     DATMRM010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11185
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2398489"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VNZ06_11920"
     CDS             complement(<1..305)
                     /locus_tag="VNZ06_11920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW21507.1"
                     /translation="MTHAAPLLSVVIPVYNEEAVLASLFARLYPALDALGRSYEIVFV
                     NDGSRDRSAGLLRAQFEARPDVTRVVLFASNFGQHRAIMAGFAHARGQYVITLDADL"
     gene            complement(319..1482)
                     /locus_tag="VNZ06_11925"
     CDS             complement(319..1482)
                     /locus_tag="VNZ06_11925"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HWW21508.1"
                     /translation="MSSKEFLPFARPSIDEATIAGVAEVLRSGWITSGPQVRDFEARL
                     SAYFNGRPVRALNSATGGMEIAMSLVGVGPGAEVITTPLSWVATANVIVRAGGTPVFV
                     DVDPRTRNMDLSRIEAAITPHTRALFPVDLAGLPVDRTRLYTIARKHKLRVVEDAAQS
                     MGASWQGERIGVIGDIVSISFHANKNITTSEGGCLVLNDEDEAKRCELWRLQGVQRFP
                     DGDLDATLPGGKYNLTDVAARIGIGQLARLDEFNVRRHLLAHRYFERFDRSLGCELPV
                     EDFSNSNWHMFQVVLPTHVKRGDFIQQMYAAGIGVGVHYPAIHLLTLYRQMGFKPGDF
                     PNAEHIGAGIVTLPLFPAMRDEDVDRVCAEITRVIKSIAKPTRVATAAVASRG"
     gene            complement(1491..1640)
                     /locus_tag="VNZ06_11930"
     CDS             complement(1491..1640)
                     /locus_tag="VNZ06_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21509.1"
                     /translation="MKGADLALILAGVLLKALAAWLLFAQVLSSARVIGIGVIIVGCW
                     LVARS"
     gene            complement(1637..3298)
                     /locus_tag="VNZ06_11935"
     CDS             complement(1637..3298)
                     /locus_tag="VNZ06_11935"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWW21510.1"
                     /translation="MSSDPAIASHPLGGRGLFIFWFLLALLWFLPLNSPHLFEPDEGR
                     YAEIPREMVASGDWVTPRLDAIKYFEKPALQYWTTAAAYKLFGEHAWTVRLWPALTGF
                     LGLLLTWSLARRIYDERAGLLAVMVQASALLYIAMARIATLDMSLGFTLQIAMTALAL
                     MVRLSTASNWRLPALLGVGVTLAVLTKGLVGILIPGATACLFMLIHRDWYLPLRARAW
                     WTLGILVVVGAPWFVLTSQRNPEFAHFFFVVQHFQRYLSPQGFDRYQPWWFFIGILAA
                     GFLPWTTFLPGALVTAVRSARAGDRASSFLVIWALFVLVFFSISQSKLAPYIVPLLPA
                     LSLLTGREIARLTPGRVSAHLSLIFLFALTVVLAMLVLWATPLGAALVARASTLSIAG
                     FLVAFGALGICCARAAYLARAGAVISAVSVTSLGSLLLTQSALMGADYLPRMHRVVEL
                     EEHLRPALTPATHVYCVNEYLQPIPFYLQRPCTLVGYRGELDFGLTQEPWRFLPDLRS
                     FATDWGEESDALAILRPEDYQQLETMGVPMRVIYTATSYVAVARE"
     gene            3370..4497
                     /gene="moeB"
                     /locus_tag="VNZ06_11940"
     CDS             3370..4497
                     /gene="moeB"
                     /locus_tag="VNZ06_11940"
                     /inference="COORDINATES: protein motif:HMM:NF004281.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HWW21511.1"
                     /translation="MALSPSELRRYSRHLSLAEIGVVGQEKLRTSSVLIVGAGGLGSP
                     AALYLAAAGIGTLGVLDFDRVDESNLQRQVLFDTASVGQSKADSARYRLAALNPELRI
                     VAHDVELRAANVQRLFADYDLIVDGTDRIATHYLVNDACVLFAKPLVTAAIYRFEGTI
                     MSYLPDRGPCYRCLFPQIDETSAPNCAEVGVLGVLPGVLGTLQATEAVKLLLGIGEPL
                     IGRLLTYDALELSFQELRFQRRADCAVCGGDPSITAPLDPPGFCSAEQLAAVRSIDAK
                     QLARELTATAVRPQLIDVREPPEFAAGHLPGSINLPMSRIEREGMTLLDPQRVIFVCR
                     SGVRSARAAALAAQAGLHGARQLAGGLLSYRDQIEPTLSVV"
     gene            complement(4501..5688)
                     /gene="pobA"
                     /locus_tag="VNZ06_11945"
     CDS             complement(4501..5688)
                     /gene="pobA"
                     /locus_tag="VNZ06_11945"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HWW21512.1"
                     /translation="MATVRTQVGIVGAGPAGLLLWHLLRRQGIEAVVIEARSRAYIEE
                     RVRAGVLEQGTVDIVQGLGLGARMQREGLQHAGIYLRFGGSSHHIDFRELVGRSVTVY
                     GQQEVVRDLIGACLESGGRIHFEVSDVALADLTGTHPLVNYRDATGAAQQLRCDFVAG
                     CDGYHGICRPSIPEDVLKVFERDYPFAWLGILAHASPVAEELIYSNHPAGFALFSMRS
                     PQVVRYYLQCRPDEDLAQWPDARVWDELDHRLEGEHCPSLKRGEVLQKSVTPMRSFVV
                     EPMRHGRLFLAGDAAHIVPPTGAKGMNLAAADVRALSMALAEFYARGSTERLDRYSEL
                     CLRRVWKAQRFSWWMTSMLHRFEQHTPFEQRLQQAELDYVCTSTQASKSLAENYVGLP
                     FED"
     gene            complement(5752..6816)
                     /locus_tag="VNZ06_11950"
     CDS             complement(5752..6816)
                     /locus_tag="VNZ06_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HWW21513.1"
                     /translation="MNTDALRVTPGFQFASDNTAGICPEACTALIEANQGYAASYGAD
                     PWTVRACDLIRKLFERPSAQVFFVFNGTASNALSLSHLTRSYHGIVCHEAAHAQSDEC
                     GAPEFFSGGAKLIALPGADAKLVPDTLQRLIDTRDDLHFPKPRALSLSQSTEWGTYYR
                     PAEIASLAQVAHQHHMLVHMDGARFANAVAACAGSTAADLSWRAGVDVLSLGGTKNGL
                     NTTEAVVFFDASLAREFEYRCKQGGQLSSKMRFQSCQWQAVLESGAWLRHASHANAMA
                     QRLGGAVREITGVKLTRPVEANAVFLDMSVKCARALDSAGWHFHAMHGAGHRLMCSWA
                     TSEADIEAFARDLRRLALTS"
     gene            6855..7781
                     /locus_tag="VNZ06_11955"
     CDS             6855..7781
                     /locus_tag="VNZ06_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HWW21514.1"
                     /translation="MYAIAIHGGAGALPKGGLSPERQREYRQSLSQALTAGQALLERA
                     GNALDAVEAAVTALEDDPLFNAGRGAVLCEDGSVELDAAIMSGSDLRAGAVAGIRYIR
                     NPIRLARRVMEKSPHVFLTGAGAEAFADVNGFERVPNEYFITPLRSQQLQALLRPAST
                     SGPAAVAALDEPPLGTVGAVARDSHGRLAAATSTGGMTGKLRGRIGDSPVIGAGTYAD
                     DRSCAVSTTGHGEWFLRTVQAYDIAARLNYGGYLLADAVNDAIAHRLTRLGATGGLIA
                     VNRDGEIVMRYNTPAMFRACVRRGEPATIEIE"
     gene            7974..9854
                     /locus_tag="VNZ06_11960"
     CDS             7974..9854
                     /locus_tag="VNZ06_11960"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWW21515.1"
                     /translation="MSLLVGFGATVSDAQPVDPAWLQQSVEAASTELRIDPETGRHQL
                     ESALKQLHVAPDADTEIRAHLALFEYYLGRDRAAAEAEANAADQLLIKASRPGLQAGV
                     LTSRAKLLVVMGDSAQAAELYDRAASIAEDTRDDEMLAEVLLSRGYLRELRGDYAHGL
                     ADLRRAQALFEQNEMPLRALLALNAIAIVYNRMGDASEATHIFERTLEALHNAGFRRD
                     EAVTLLAQGEAYENLQQFALARSAFQAALELARQMDNMRAAGYALRGLASVASENENP
                     NGALSMLDRAADLQQQISDARLQAQIELTRGIALHRLSRLRQSHAALSQALTLFHQIG
                     SQSELATTYGELAAVSGELGNWRDAYEYRSLSQTLTTQLLRSQLDQRFATLKVEYDTA
                     SKEKENELLIRENVADQAALAQRAKAGNLQTAVIVLVVLLLGVLATLALHQRRASSRL
                     RLLAMTDELTGVPNRRAVLTLLSQILRRSPDPISILIIDLDHFKSINDRHGHLIGDEA
                     LKRVAADLRDAVIDPAFFGRLGGEEFAAVLPATRIEAASAIAELLRERVLRLDLSRWL
                     GDRRITVSIGIATSLPGRDSVTTMLRRADSALYSAKDAGRNCVRTKSSEEGERELAPR
                     VA"
     gene            complement(9875..10840)
                     /locus_tag="VNZ06_11965"
     CDS             complement(9875..10840)
                     /locus_tag="VNZ06_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWW21516.1"
                     /translation="MASAEVTLQIIQAARTRIAGWVRRTPVLESETLNTLAGARLYFK
                     CENFQEGGAFKARGAANAVFSLSEEDLRAGVVTHSSGNHGAALARAARLRGVRAQIVM
                     PENSSAAKFEAVRSFGGEVLTCAPTLAAREAMAEQIIRQSGAVFVHPYDDPRVIAGQG
                     TIALELFEDVPELDVLLLPVGGGGLASGISTAAKGLRSTIRVVAVEPDGADDAYRSFV
                     SGKLTGNTRLDTIADGLRGALSVRTFGLIREHVDQIVTVSEAAIVRAMRTLWQQLKIV
                     VEASAAVPYAAVLDHRVDVREQRVGIVLTGGNVDLDTLPWVMTKP"
     gene            10924..>11185
                     /locus_tag="VNZ06_11970"
     CDS             10924..>11185
                     /locus_tag="VNZ06_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21517.1"
                     /translation="MNPLKHMRIGYRLGVVFGFTLLMFVALLALALAEEFGMHSALAY
                     YDQGVVPSIRAIHTALEGVDTTRRYEVQHLLSNDAAELSGLEG"
BASE COUNT         2148 a         3624 c         3517 g         1896 t
ORIGIN      
        1 tgcagatcgg cgtcgagcgt gatcacatac tggccgcgcg catgcgcaaa gcctgccatg
       61 atcgcgcgat gctggccgaa gttgctggca aacagcacga cgcgcgtgac atcgggccgc
      121 gcttcgaact gtgcgcgcaa cagtcctgcc gagcgatcgc ggctgccgtc gttgacgaac
      181 acgatctcat agctacgacc gagcgcatcg agcgccgggt agagtcgcgc gaacagactc
      241 gccagcaccg cttcctcgtt gtacacggga atcacaacgc tcagcagtgg cgctgcgtga
      301 gtcatgatcg cccgagtatc agccgcggga ggcgacagcg gcggtggcga cgcgtgtggg
      361 cttggcaatg gatttgatga cacgtgtgat ctcggcgcac actcgatcga catcctcatc
      421 gcgcattgcc gggaacagcg gcagcgtcac gataccggct ccaatgtgct cggcgttcgg
      481 aaaatcgccc ggtttgaatc ccatctggcg atagagggtc agcagatgaa tcgctggata
      541 gtgcacgccc acgccgatac cggcagcgta catctgctgg atgaaatcgc cgcgcttcac
      601 gtgcgtcggc aagaccacct gaaacatatg ccaattgctg ttgctgaaat cctcgaccgg
      661 cagctcgcag ccgagcgagc gatcaaaacg ctcgaaataa cggtgcgcca gcaggtggcg
      721 gcgcacattg aactcatcga gccgggcgag ctgtccaatg ccgatgcgcg ccgcgacatc
      781 ggtcagattg tatttgcccc ccggcagcgt ggcatccaga tccccgtctg gaaaacgctg
      841 cacaccctgc agccgccaca gctcgcagcg cttggcctcg tcctcatcgt tgagcaccag
      901 acagccaccc tcggaagtgg tgatgttctt gttggcgtgg aagctgatcg atacgatgtc
      961 gccgatgacg ccgatgcgtt ctccctgcca gctcgctccc attgactgcg cggcgtcctc
     1021 gaccacacgc agtttgtgtt tgcgcgcgat ggtgtacaga cgggtgcgat cgaccggcaa
     1081 accggccagg tccacaggaa acagtgcgcg ggtatggggt gtgatcgcgg cctcaatgcg
     1141 cgacaaatcc atgttgcggg tgcgcggatc gacgtcgaca aacaccggcg tgccgcccgc
     1201 tcgcacgatg acgttggcgg tcgcgaccca ggacaacggc gtggtgatga cttcagcgcc
     1261 cggtcccaca ccgaccagcg acatcgcgat ctccataccg ccagtcgcgg aattgagcgc
     1321 acgcaccgga cggccattga agtacgccga cagccgcgct tcgaaatcgc gcacttgcgg
     1381 gccgctggtg atccagcccg aacgcagcac ctcagcgaca ccggcgatgg tcgcctcatc
     1441 gatcgacgga cgggcgaagg gtagaaactc tttgctactc acatgtgcct ctagctgcgc
     1501 gccacgagcc agcagccaac gataatgact ccgataccga tcacacgcgc ggagctcagc
     1561 acttgcgcaa acagcagcca cgctgccagg gccttgagca gcacccctgc cagtatcagc
     1621 gccagatccg cgcccttcat tccctcgcca ccgccacgta ggacgtcgca gtgtatatca
     1681 ccctcatcgg cacgcccatg gtctcaagct gttgataatc ctcaggacgc aggatagcca
     1741 gcgcgtcgct ttcctcgccc cagtcggtgg caaagctcct gaggtccggc agaaagcgcc
     1801 agggttcctg ggtcagccca aaatccagct ccccgcggta gcccaccagc gtgcacgggc
     1861 gctgcaggta gaagggaatc ggttgcaggt actcgttcac gcagtacacg tgagtcgccg
     1921 gggtcagtgc gggtcgcagg tgttcctcga gttccaccac gcggtgcatg cgcggcaggt
     1981 agtcagcgcc catcagcgcc gactgggtaa gcagcaggct accgagcgac gtcaccgaaa
     2041 cggcactgat cacggcgccg gcgcgcgcca ggtaggccgc gcgggcgcag cagatcccga
     2101 gcgcgccgaa cgccaccaga aagccggcga tactgagggt tgaggcacgc gcgaccagcg
     2161 cagctcccag gggggtggcc cacagcacca gcatcgcaag cacgaccgtc agggcaaaca
     2221 ggaatatcag gctcaggtgc gccgacacac gcccgggtgt caggcgcgcg atctcacggc
     2281 cggtcagcaa actcagcgcc ggcagcagcg gtacgatgta gggcgccagc ttggattgcg
     2341 agatgctgaa gaatacgagc acgaatagcg cccagatgac caggaacgag ctggcgcgat
     2401 cgccggcgcg cgccgatcgc accgccgtca ccagcgcacc cggcagaaat gtggtccagg
     2461 gcagaaatcc ggcagccagt atcccgatga agaaccacca cggctggtag cggtcgaatc
     2521 cctgcgggct cagatagcgc tggaaatgct ggaccacgaa aaagaaatgc gcgaattcgg
     2581 gattgcgctg tgaggtcaga acgaaccacg gggcgcccac gaccaccaga attcccagcg
     2641 tccaccaggc acgcgcccgc agcggcaaat accaatcgcg gtggatcagc atgaacaggc
     2701 aggctgtcgc gccgggaatg aggatcccga cgagtccttt ggtcagcacc gccagcgtca
     2761 cgcctacgcc gagcagcgcc ggcagcctcc agttcgatgc cgtggagagc ctcaccatca
     2821 gtgccagcgc cgtcatcgcg atctgcagcg tgaaccccag ggacatgtcg agtgtcgcga
     2881 ttcgcgccat cgcgatgtaa agcagggcgc tggcctgcac catgaccgcc agcagtccgg
     2941 cgcgctcgtc gtagattcgc cgcgccagcg accaggtcag caacaatccc agaaagccag
     3001 tcagcgccgg ccatagccgc accgtccagg catgctcgcc aaagagcttg tacgccgcgg
     3061 cggtagtcca atactgcagc gccggtttct cgaagtactt gatggcatcc aggcgtggcg
     3121 tgacccaatc cccgctggcc accatttcac gcgggatctc ggcgtagcgc ccctcatccg
     3181 gttcgaacag gtgcggcgag ttcagcggca ggaaccacag cagcgcgagc agaaaccaga
     3241 agatgaacaa gccgcggccg cccaacgggt gcgaggcaat ggctgggtca gatgacacgg
     3301 cggacacggc tgcggttcat aatcgagcag tttgacaggc ttatgccgaa gcggcgatgc
     3361 tgaaccgcca tggcgctctc gccctccgag ctccggcgct acagccgcca cctgtcgctc
     3421 gctgaaattg gcgtagtcgg ccaggagaag ctgcgcacct cgagtgtgct gatcgtgggt
     3481 gcgggtgggc tcggttcgcc ggctgcgctc tatctggccg cagcgggcat tgggacgctg
     3541 ggagtgctgg atttcgatcg ggtcgatgaa tccaacctgc agcgccaggt gctgttcgac
     3601 actgcaagtg ttggccaatc caaagccgac agcgcccgct accggctcgc ggccctcaat
     3661 cccgagcttc gtatcgtcgc tcacgatgtc gagctgcgag cggccaacgt gcaacgcctg
     3721 tttgctgact acgatctgat cgtggacggc acggatcgca tcgctacgca ctatctggtg
     3781 aatgacgcat gcgtattgtt cgccaaacca ctcgtgacgg cagcgatcta tcgcttcgaa
     3841 ggcacgatca tgagctatct gcccgatcgc ggaccgtgct accgatgtct gtttcctcag
     3901 atcgatgaaa cctccgctcc gaactgcgcg gaggttggcg tgctgggtgt cctgccgggt
     3961 gtgctgggca cgttgcaggc cacggaagcg gtgaagctgc tgctcggaat cggcgagccg
     4021 ctgatcggac gactgctcac ctacgatgca ctggaactga gctttcagga gctgcgcttc
     4081 cagcgccgcg ccgactgtgc cgtctgcggc ggcgaccctt cgatcaccgc gccgcttgat
     4141 ccgccgggat tctgcagcgc cgagcaactg gccgccgtac gctcgatcga tgcgaagcag
     4201 ctggcgcgcg agctcaccgc caccgctgtg cgcccgcagc tgatcgatgt gcgtgaaccg
     4261 ccggaattcg ccgctgggca tctgcccggt tctatcaatc tgccgatgag tcgcatcgaa
     4321 cgcgagggca tgacgctgtt ggatccgcag cgcgtcatct tcgtatgccg cagcggcgtg
     4381 cgcagcgcgc gtgccgccgc gctcgccgct caggctggcc tgcacggtgc tcgccaactg
     4441 gccggaggtc tgttgtccta tcgggaccag attgaaccga cgctgagcgt ggtgtgagga
     4501 tcagtcctcg aacggcaaac cgacatagtt ctccgccagc gacttggacg cttgcgtcga
     4561 cgtgcacacg tagtccagtt cagcttgctg taggcgctgc tcgaacggtg tgtgctgttc
     4621 aaaccgatgc aacattgagg tcatccacca ggagaagcgc tgcgctttcc acacccgacg
     4681 caggcacaac tccgagtagc gatcgagacg ctccgtactg ccccgggcat agaactcagc
     4741 gagtgccatc gacagtgccc gcacgtccgc cgcggccagg ttcatcccct tggcacccgt
     4801 cggtgggacg atatgcgctg catcgcctgc gagaaacagc cgtccgtgcc gcatgggctc
     4861 gaccacaaaa ctgcgcatcg gtgtgacgct cttctgaagg acctcaccac gcttcagaga
     4921 cgggcaatgc tcgccctcga ggcggtggtc cagctcgtcc cagacccgcg catcaggcca
     4981 ctgagccagg tcctcatccg ggcggcactg caggtagtag cgcacgacct gcggcgaacg
     5041 catgctgaag agcgcgaagc cggccggatg attcgagtag atgagttcct cggcgactgg
     5101 agaggcgtgg gccagaattc cgagccaagc gaacggatag tcgcgctcga agaccttcag
     5161 aacatcttcc gggatcgagg gacggcaaat gccgtggtag ccatcgcaac cggcgacgaa
     5221 atcgcaccgc agctgctgtg ccgcgcccgt ggcgtcgcga tagttcacca gcgggtgcgt
     5281 gccggtcaga tcggcgagcg cgacatcgct gacctcaaaa tgaatgcggc cgccggattc
     5341 gaggcaggcg ccgatgagat ccctgaccac ttcctgctgg ccgtagaccg tgacactgcg
     5401 gccgaccagc tctcgaaaat cgatatgatg gctcgagccg ccgaaacgca gatagatccc
     5461 ggcgtgctgc aacccctcgc gctgcattcg agcgccgagt cccaggccct ggacgatgtc
     5521 gaccgttccc tgttcgagca ccccggcgcg tacccgctct tcgatgtacg cgcggctgcg
     5581 cgcttcaatg acgacggcct cgatgccctg tcgacgcaac aggtgccata gcagcagacc
     5641 ggcgggtcct gcgccgacaa tgcccacctg cgtcctgacg gtagccacca cggcacggta
     5701 gccgaaggcc cgcggtcgct aatgtccaaa aaatactacc gacttgtact tttatgacgt
     5761 caacgccaac cgacgcagat cccgtgcgaa ggcttcgata tccgcttcgc tcgtggccca
     5821 ggagcacatc aggcggtgac ccgcgccgtg catggcgtga aagtgccaac cggcactatc
     5881 cagcgcccgc gcgcatttca cgctcatgtc cagaaacacc gcgttggcct ccaccgggcg
     5941 tgtcagcttc acgccggtga tctcacgcac cgcgcccccg aggcgctgcg ccatcgcatt
     6001 ggcgtgcgac gcgtgacgca gccacgcacc ggactcgagt accgcctgcc actggcagct
     6061 ctgaaatcgc atcttgctgg agagctgtcc gccctgcttg cagcgatatt cgaactcgcg
     6121 cgccagcgac gcatcgaaga agaccaccgc ctccgtggtg ttcagcccgt tcttggtgcc
     6181 acccagcgac agcacgtcca cgcccgcacg ccagctcaag tccgccgccg tgctgccggc
     6241 gcaagcagcc actgcattcg caaaacgcgc gccatccata tgcacgagca tgtggtgctg
     6301 gtgcgcgacc tgcgccagcg aggcgatttc ggcggggcgg tagtaagtac cccattccgt
     6361 ggactggctt aggctcaaag cccgcggctt cgggaaatgc aggtcgtcgc gtgtgtcgat
     6421 caagcgctgc agcgtatccg gtacgagttt tgcgtccgct ccgggcaagg cgatgagctt
     6481 tgcgccgcca ctgaaaaact cgggcgcgcc acattcatcg gactgtgcgt gcgcggcctc
     6541 gtggcagaca atgccgtggt agctgcgggt caggtgcgac agcgacagcg cattggacgc
     6601 ggtaccgttg aaaacgaaaa acacctgtgc tgacggacgt tcgaacagct tacggatcag
     6661 atcgcaggcg cgcacggtcc agggatctgc gccgtagctg gcagcgtagc cctgattggc
     6721 ctcaatcaac gcagtgcatg cctcgggaca gataccggcc gtgttgtcgc tggcgaattg
     6781 aaacccggga gtcacacgca aagcatcggt attcatgcgc acaggctagc gcagttcgcg
     6841 tacgcttgcg tgccatgtac gcgatcgcga ttcatggggg agccggcgcg ctcccgaagg
     6901 gcggactgag ccccgagcga caacgggaat accgccaatc actgtcgcag gcactgacgg
     6961 ccggtcaggc cctgctcgag cgcgcaggca atgcgctgga cgctgtggaa gcggcagtca
     7021 ccgcgctcga agacgatcca ctcttcaatg ccgggcgggg cgcggtgctg tgtgaggacg
     7081 gaagcgtcga gctcgatgcc gccatcatga gcggcagcga tctgcgagcc ggtgcggtag
     7141 cggggatccg atacatcaga aacccgatcc ggctggcgcg acgcgtgatg gaaaaatctc
     7201 cccacgtttt cctgacgggt gcgggtgccg aagccttcgc cgatgtgaac ggctttgagc
     7261 gagtgccgaa tgaatacttc atcacacctt tgcgctcaca acagctgcag gcgctgctgc
     7321 gaccggcatc gacttcaggg ccggccgcag tcgccgcgct cgatgagcct ccgctgggaa
     7381 ccgtgggggc agtcgctcgc gactcgcatg gccggctggc tgcggcaact tcaactggcg
     7441 gaatgacggg caaactgcgt ggccgcatcg gcgattctcc ggtcatcggc gcgggcacct
     7501 acgccgatga tcgcagttgc gcggtctcta cgaccgggca cggcgaatgg ttcctgcgaa
     7561 ccgttcaggc ctacgacatc gcagcgcgct tgaactatgg cggctacctg cttgccgatg
     7621 cagtgaatga tgcgattgca catcgtctga cgcgattggg cgccaccggc gggttgatcg
     7681 ccgtcaatcg cgacggtgag atcgtcatgc gctacaacac gccggcgatg ttccgcgcct
     7741 gtgtgcgccg cggcgaacca gccacaatcg agattgagta gcaagcgaaa tcgccgcgcc
     7801 tgtcctgctt atgtcacacc ggacgccggc atccgccgac tgcccacaat tacagccacg
     7861 cgcgagcggc aagcttttag aatagcggtc tgcctcgaac aggataaaga cggataactc
     7921 gattgccgat tcgtcgcgtc cgcgtcaccg ctcggtctgg cgtgctggca gccttgtcgc
     7981 tgttggttgg ctttggcgcg actgtcagcg acgcgcagcc cgtggacccg gcttggctgc
     8041 agcaatcagt ggaagcggcg agcaccgaac tccgcatcga tcctgagacc gggcgtcacc
     8101 agctcgaatc tgcgctgaag cagctgcacg tcgcgcccga tgccgacacg gagatccgtg
     8161 cgcatctggc tctgttcgag tactacctcg gacgcgatcg tgctgctgct gaggccgaag
     8221 ccaacgccgc ggatcagttg ctgatcaagg cctcgcgtcc gggtctgcag gcgggcgtat
     8281 tgacgagccg cgccaagctg ctggtcgtca tgggtgatag tgcgcaggcc gccgagctgt
     8341 acgatcgggc agcgtcgatc gccgaggaca cccgcgatga tgaaatgctg gccgaagtgc
     8401 tgctgtcgcg cggctatctg cgcgagctgc gcggcgacta cgcgcacgga ttggcggatc
     8461 tgcgccgcgc gcaggcgctg ttcgaacaga atgagatgcc gctgcgagct ctactggcac
     8521 tgaatgcgat tgcgatcgtc tataaccgca tgggcgacgc cagcgaggcg acccacattt
     8581 tcgagcgcac gctcgaagcg ctgcacaatg ccggattcag acgcgatgag gcagtcaccc
     8641 tgctggcgca gggcgaggcg tatgaaaacc tgcagcagtt cgctctggcg cgcagcgcct
     8701 ttcaagcagc gctggagctg gccaggcaga tggacaatat gcgcgctgcg gggtatgcac
     8761 tgcgcgggct cgcaagtgtc gccagcgaga atgagaatcc caacggcgcg ctctcaatgc
     8821 ttgaccgcgc cgcggacctt cagcagcaga tctccgacgc gcgcctgcag gcgcagatcg
     8881 agctgacccg tggcattgcg ttgcatcggc tgagccggct gcgccaaagt cacgctgccc
     8941 tgtcgcaggc gctgaccctg tttcaccaga tcggctcgca aagcgagctg gcgacgacct
     9001 atggcgagct ggcggccgtc agcggcgaac tcggcaactg gcgcgatgcc tacgaatacc
     9061 gcagcctgtc gcagacgctc accactcagc tgctgcgcag ccagctcgat cagcgctttg
     9121 caacgctcaa ggtcgaatac gacaccgcgt cgaaggaaaa agagaacgag ttgttgatcc
     9181 gcgagaacgt ggcggatcag gccgcgctcg cgcagcgtgc caaggccggg aatctgcaaa
     9241 ccgccgtgat cgtgctggtc gtgttgctgc tgggcgtgct ggcaacgctg gcgctgcacc
     9301 agcgccgggc gtcctcgcgc ttgcggctgc tggcgatgac tgacgagctg accggcgttc
     9361 cgaatcggcg ggcggtactg acgctgctat cgcagatact gcggcgcagc ccggatccga
     9421 tctcgatcct gatcatcgat ctggatcact tcaagtccat caacgaccgc catggccacc
     9481 tgatcggcga tgaagcactc aaacgcgtag ccgcggactt gcgcgacgcg gtgatcgacc
     9541 cggccttctt cggccggctg ggcggcgaag aatttgccgc cgtgctcccg gccacgcgga
     9601 tcgaagcggc cagcgcgatc gcagaattgc tgcgcgaacg ggtactgcgg ctggatctgt
     9661 cgcggtggct gggtgatcgg cgcatcaccg tcagcatcgg catagcgacc tcgctgccgg
     9721 gacgtgattc ggtcaccacg atgctgcggc gggcggacag cgcgctctat tcagcgaagg
     9781 acgcgggccg caactgtgtg cgcaccaaat cgagcgaaga aggggaacgc gaacttgcgc
     9841 cgcgcgtggc gtgagcggat ggtcgactga ccgcctaggg cttcgtcatc acccacggga
     9901 gcgtgtccag gtcgacattg ccgccggtca gcacgatgcc gacgcgctgc tcgcgcacgt
     9961 ccacgcgatg gtcgagcact gccgcgtagg gcaccgcggc tgaggcctca accacaatct
    10021 tcaactgctg ccagagggtg cgcatcgcac gcacgatcgc cgcctcgctc actgtcacga
    10081 tctgatccac atgctcccgg atcaggccaa acgtacgcac actcagggcg ccgcgcagcc
    10141 cgtcggcgat cgtgtccagc cgcgtattgc cggtcagctt gccggagaca aaggagcgat
    10201 aggcatcgtc ggcaccgtct ggttccacgg ccaccacccg aatcgtcgag cgtagccctt
    10261 ttgctgccgt tgaaataccg ctggccagcc cgccaccgcc gaccggcagc agcagcacat
    10321 ccagctcagg cacgtcctcg aacagctcca gagcaatcgt gccctgaccg gcgatcacgc
    10381 gcggatcgtc gtacgggtga acaaacacgg cgccgctctg acgaatgatc tgctcggcca
    10441 tggcctcgcg cgctgccagc gttggcgcgc acgtcagcac ctcgccgcca aagctgcgta
    10501 ccgcctcgaa tttggccgct gatgagttct ccggcatcac aatctgcgct cgcacaccac
    10561 gcagccgcgc cgcccgcgcg agggcggcgc cgtggttgcc ggatgagtgt gtcacgacgc
    10621 cggcgcgcag atcctcctct gacagcgaga acaccgcatt ggcggcacca cgtgccttga
    10681 aggcacctcc ttcctgaaag ttctcgcatt tgaagtacaa gcgtgcgccg gcgagtgtgt
    10741 tcaaagtctc actttccaga acgggggtgc ggcgcaccca acccgcgatt cgggtgcgtg
    10801 ccgcctggat gatctgcagt gtgacctcgg cggatgccat atgcatgtct cttaccgata
    10861 agcatagcga atcggcgact acctcacaga ccgggaagcc ggggacgttc tattttgtta
    10921 agtgtgaacc ccttgaagca tatgagaatc ggctatcgat tgggagtcgt cttcggcttc
    10981 acgctgctga tgttcgtggc gctgctggcg ctggcgctgg cggaagaatt cggcatgcat
    11041 tccgcgctcg cgtactacga ccagggcgtg gtgccctcga ttcgcgcgat ccacacggca
    11101 ctggaaggcg ttgacaccac acggcgatac gaggttcagc atttgctgtc caatgatgcc
    11161 gccgaattga gcggcctgga aggca
//
LOCUS       DATMRM010000396         4039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3307325, whole genome shotgun sequence.
ACCESSION   DATMRM010000396 DATMRM010000000
VERSION     DATMRM010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3307325"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VNZ06_11975"
     CDS             <1..196
                     /locus_tag="VNZ06_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21518.1"
                     /translation="SIRAPYGCEIEETRCPVFTCLKRAHENFHVLPKAGCGIGCFRRR
                     RCRMILDPDCVGGIINMSRV"
     gene            629..1630
                     /locus_tag="VNZ06_11980"
     CDS             629..1630
                     /locus_tag="VNZ06_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWW21519.1"
                     /translation="MLVRRGEAAALRRLIEFLLREKVIPAQKVPPARLTAVEHCVQDF
                     ESYLRDERVLARATIVNYVPFIREFLQGRFGRERVKLSRLRAGDVVRFVQRQALRLHM
                     RRAKLMTTALRSFLQYTRYRGEASLDLAAAVPAVANWSMTSVPRGIAPDQVRQLLASV
                     DRATPIGRRDYAILLLLARLGVRSGEAASLELDDIDWSLGLLSVRGKGGRRNELPLPA
                     DVGKAIAEYLRRGRPPSTSRRVFLRAKAPIRGFIGATGVGAVVQHCIRRAGIKAPTHG
                     AHQFRHGLAAQMLRKGASLSEIGDLLGHRHPQTTMIYTKVDLVALRSLALPWPGSAP"
     gene            1627..2580
                     /locus_tag="VNZ06_11985"
     CDS             1627..2580
                     /locus_tag="VNZ06_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWW21520.1"
                     /translation="MNTLRQAVADYLRMRRGLGFKLIDAGKGLCDFVTFMQQRRAPYI
                     TQALALAWAQQPSKARPTRWAQRLCFVRGFARHRSATDPRTQIPPPGLLPFRPKRARP
                     YLYSDQEIEQLLRATLNMPLSARHCKSSALLPWVYYCLFGLLSVSGLRLCEARNLELR
                     DVDLKAAVLTIRGTKFGKTRLVPLHASTCAVLATYIARRKRHWARRAVSSYLFVSSRG
                     NRLDGGQIHRAFYASSRQIGLRSASDSRGPRLHDMRHRFATNTLVRWYKSEQDPERRL
                     PILCAYLGHVHVADTQWYLDGSPQLMREAIGRLEHRWEARQ"
     gene            2577..3575
                     /locus_tag="VNZ06_11990"
     CDS             2577..3575
                     /locus_tag="VNZ06_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWW21521.1"
                     /translation="MSAPTSFASLLQCFFTQRLMQQRQASPHTISSYRDTFRQFLKFI
                     QLRLHKPPSRLHLEEIDAPLIVAFLDDLEKHKGLSVRSRNLRLTAIHSFFRYAAFEAP
                     AHSIQIQRVLAIPSKRFTRTLVSFLTRAEVEALLAAPDRRHWSGRRDHAFVLVAVQTG
                     LRLSEMTGLKREDLILGAGAHVRVIGKGRKERCTPLTRATLAVLSTWLREPQRGTDDV
                     LFPSARGRRLSVHGVQYLLNKHRASACASCPSLKKKRVTVHRLRHTMAMDLLQAGVDR
                     AVIALWLGHESVETTQIYLEATIAMKEQALAKTAPLQAKPGRYHPADRLLSFLNTL"
     gene            complement(3632..>4039)
                     /locus_tag="VNZ06_11995"
     CDS             complement(3632..>4039)
                     /locus_tag="VNZ06_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWW21522.1"
                     /translation="SGLRDRAMLETLYSTALRRMELPGLALYDVDLTRRLVMVREGKG
                     KRDRVVPIGARAAAWVDKYLQEARPQLLIAESESLFLTDYGEPVTPEYVAERVRRYMH
                     FAGIQKPGACHLFRHYLPYRTMSSNLRSIATFA"
BASE COUNT          746 a         1307 c         1189 g          797 t
ORIGIN      
        1 gagtattcgc gctccctatg gatgcgagat cgaagagact cggtgcccgg tgttcacctg
       61 cctgaaacga gcgcacgaaa atttccacgt gctgccgaaa gctgggtgtg gcattgggtg
      121 tttccgtcgg cgacgctgtc gcatgatcct cgatccggac tgtgtcggcg gcatcatcaa
      181 tatgagcagg gtgtgagccg agcgatcact cgcgcaagcc atcgagcggg gatcacgagg
      241 cgcgtgactg cgcacacgct gcggcactat ctaccgttcc tgactatgtc tgctacttcg
      301 atggcaagct gatttcgccg gctgattcgc gagtctgtgt agacatagtc ttgactcctt
      361 gcatcacccc agatcgggaa aggagtcacc catgcagtac atcatcagtg ccgacggcgt
      421 tctatcgtcc acacttaagg gcccgttagc gccccatctt gcctcgtttg ccgagcacgt
      481 gagtgcgctc ggatacaccc tggacagcct gcagaagcag gttcggatcg cggctttctt
      541 tagccgctgg ctccaacgca acggtattgc gttgcgcgaa gtttcatccg gccatgcaca
      601 acggttcctg cgacattgca accgacggat gcttgtccgc agaggtgagg ctgcagcgct
      661 cagacggctc attgagtttc tactgcgcga gaaggttatt cccgcgcaga aagtgccacc
      721 ggctcggtta acggccgtcg agcattgtgt gcaggacttt gagagctacc tgcgggatga
      781 gcgcgtcttg gcgagagcca cgatcgtgaa ctacgtgcca ttcatccgcg agtttctcca
      841 gggtagattc ggccgggagc gcgtcaagct ctcgcgcctg cgtgccggcg atgtcgtgag
      901 atttgtccag cgccaggcgt tacgcctgca catgaggcga gcgaagctaa tgaccactgc
      961 gttgcgctcg ttcctgcagt acacacgcta tcgcggagag gcgagcctgg atctggccgc
     1021 tgccgttccg gccgtggcga actggtctat gacgtcggtt ccgcggggga ttgcgccgga
     1081 tcaggttcga cagctactcg ccagtgtcga tcgggccacg ccaatcggtc gtcgcgacta
     1141 cgccatcttg ctcctgctcg caagactggg cgtgcgctcg ggcgaagcag catctcttga
     1201 gctcgatgac attgattgga gcctcggact gttgagcgtt cgcggcaagg gcggtcggcg
     1261 caacgagctg cctttacctg ccgacgtcgg caaagcaatc gctgagtacc tgcggcgtgg
     1321 ccgaccgccc agcaccagtc ggcgcgtgtt tctgcgcgcg aaggcgccga tccgtggctt
     1381 tatcggtgcc accggcgttg gggcggtcgt tcagcattgc atcaggcgcg ccggcatcaa
     1441 ggccccgacc cacggcgcac atcagtttcg gcacggattg gccgcccaga tgctacgcaa
     1501 gggcgcatcg ctcagcgaga tcggcgactt gttgggccat cgtcatccgc agacgacgat
     1561 gatctacacc aaggtcgatc tggtggcact gcgctcactg gcgctgccct ggccagggag
     1621 tgcgccatga acacgctgcg acaggccgtt gccgattatc tgcgtatgcg tcgtggtctt
     1681 ggattcaagc tgatcgatgc gggcaaggga ttgtgcgatt tcgtcacctt catgcaacag
     1741 cgccgcgccc cctacatcac ccaggcattg gctcttgctt gggctcagca accttccaag
     1801 gcccgcccga cgcgctgggc acaacgcctg tgcttcgtac gcggcttcgc ccgccatcgc
     1861 agcgcaaccg atccacgcac gcagattcca ccgcccggat tgttgccgtt ccgaccgaag
     1921 cgggctcggc cgtatctgta ctcggatcag gagattgagc agttactgcg tgccacgctc
     1981 aacatgcccc tgtcagcacg ccactgtaag agcagcgcat tgttgccgtg ggtgtactac
     2041 tgtctatttg ggctgctgag cgtctcgggc ctgcgcctgt gcgaagcacg caacctcgag
     2101 ctccgggacg tcgatcttaa ggcagccgta ttgacgatcc gcggcaccaa gttcggcaag
     2161 actcgtctgg tgccgctcca tgcctcgacc tgcgcggtgc tcgcaaccta catcgccaga
     2221 cgcaagcgtc attgggcgcg acgggcggta tcatcctacc tattcgtctc cagccggggc
     2281 aatcgcctcg atggcggaca gattcaccga gccttctatg cgtcgtcgcg acagattgga
     2341 ttgcgcagcg cttccgacag ccgcggcccg cgcctgcacg acatgagaca tcgcttcgca
     2401 accaacacgc tcgtgcgctg gtacaagtcc gagcaggatc ccgagcgccg cctgcccatc
     2461 ttatgcgcgt accttggcca tgtgcacgtt gccgataccc agtggtatct ggatggctca
     2521 ccgcagttga tgcgcgaggc gatcggccgc ctcgagcatc gctgggaggc tcgccaatga
     2581 gcgcacccac cagctttgcc tcgctactgc agtgtttctt tactcagcgc ctgatgcagc
     2641 agcgtcaagc cagcccgcac acgatcagct cctatcgcga taccttccgg cagttcctaa
     2701 agttcattca gctgcggctg cacaagccac cgtcccgcct gcaccttgag gagatcgatg
     2761 ctccgctgat cgtcgctttt ctcgacgacc tggagaagca caaaggcctc agtgttcgca
     2821 gccgcaacct gcgcctgacc gcgattcact ccttcttccg ctacgccgct tttgaggccc
     2881 ccgcgcactc catacagatc cagcgggtgc ttgccatccc cagcaagcga tttacccgca
     2941 cgctggtctc cttcctcacc cgcgctgagg tagaagcgct gctggccgct ccagatcggc
     3001 gccactggtc tgggcggcgt gatcacgcat ttgtcttggt cgcggtacag accggactgc
     3061 gcttgtcgga gatgaccggt ctcaaacgtg aagacctcat tctcggggcc ggcgcccatg
     3121 tgcgcgtcat cggcaagggc cgcaaggagc gctgtacacc gctcaccaga gcgacgctcg
     3181 ccgtactaag cacatggctc cgagagccgc aacgtggtac cgacgacgtg ctgttcccca
     3241 gtgccagggg acgacgtctg agcgtccacg gtgtgcagta cctgctgaac aagcatcgtg
     3301 cgagcgcctg cgcatcatgc ccttcactga agaaaaagcg cgtcaccgtc caccgcctga
     3361 ggcacaccat ggccatggac ctcttgcaag cgggcgtcga tcgcgcagtg attgctctgt
     3421 ggctcggtca cgaaagcgtc gagacgacgc agatctacct cgaagcgaca atcgcgatga
     3481 aggagcaagc cctcgcaaag accgcgccgc tacaggcaaa gccaggtcgc tatcaccccg
     3541 ctgatcgact tctgagcttc ctcaatacct tgtgaaacaa aggactatgt cgggtgcatg
     3601 accggcgcct ccatcgcgtc ggcctataga actaggcaaa agtcgcgatg ctccgcaaat
     3661 tactcgacat agtccggtag ggtagatagt ggcggaacag gtggcatgca ccgggctttt
     3721 ggataccagc gaagtgcatg taacggcgca cgcgctcggc cacgtactcc ggggtcaccg
     3781 gctcgccgta atcggtcaag aacagcgact cgctctcggc gatcagcaac tgcggtcgcg
     3841 cctcttgcag atatttatcg acccacgccg ccgcacgcgc accgatcggc accacccgat
     3901 cgcgcttgcc cttgccctcg cgcaccatca ccagccggcg cgtgaggtcc acgtcataga
     3961 gcgcgagccc tggcagctcc atgcgccgca acgccgtcga gtacagcgtc tccagcatcg
     4021 cccgatcgcg cagtcctga
//
LOCUS       DATMRM010000397         8230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_560241, whole genome shotgun sequence.
ACCESSION   DATMRM010000397 DATMRM010000000
VERSION     DATMRM010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560241"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VNZ06_12000"
     CDS             <1..703
                     /locus_tag="VNZ06_12000"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HWW21523.1"
                     /translation="SMLLAFLGVLLLRRAPRLGRALVVIGIASIWLLSTPIVADQLTV
                     WVEHYAPLDLRQPTGAQAIVILGGGGQRRYAPEYAGPEAEPYLLERVAYGAFVAQRTG
                     LPILVTGFGIEALAMRESLKRHFSIEARWVDDQSYDTFENARNSVRILKSAGIQRTIL
                     VTRSTHLWRSVHEFEAAGIEVVPAPVGSFAVRDLNIFRFLPDTEALTRSHAAIYEALG
                     DLVRRALATTHLRRH"
     gene            753..2798
                     /locus_tag="VNZ06_12005"
     CDS             753..2798
                     /locus_tag="VNZ06_12005"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HWW21524.1"
                     /translation="MSPDNPFFHPSALPYQFPPFDRIRSTDFVPAYLAGIEQHRQEVQ
                     AICANPAPATFDNTIVALERSGRLLDRVDSAFSNLSSSDGDAAMLQIESEMAPRLAAH
                     EDAIYLLPPLFARIEQLYAHRGQLKLDAESLQLLERYRVQFVRAGALLSERDKSRLRE
                     LNSRLSTLTTQFRQNVLRSSRDSAVLIDDEAQLDGLAPDELAAAAANAAARGLGGKWV
                     IALQNTTTQPLLSQLTRRDVRERIFRVSTQRARGGEADNTGIIAEIVRLRAERARLLG
                     CPDHATYVLKDETAGSSAAVDAMLQRIAAAALYYARQQAAGIEQLIGAQSTLQPWDWD
                     FYAAQVRKTKYDFDLSQVKPYFELNRVLQDGVFFVASELFGLSFVERHDLPAYHPDVR
                     VFEVFEADGSALGLFVADFYARDNKQGGAWMNHFAVQSQLLGRKPVVVNQLNIPKPAS
                     GAPSLLTFEDVTAMFHEFGHALHGLLSNVCFPLLSGIRVPRDFVEFPSQYNEMWTREP
                     AVLANFARHYQTGAAMPTALLEKVLAAQNFDQGHRTSEYVQSAIIDQAWHRLSAEQTP
                     PAAEVVQFEADILRRRGVAYDPIPPRYHSWYFLHIFSGGYSAGYYAYIWSEVLARNVG
                     RWLHERGGLTRANGQCLREKILSRGRTVDPIRLFEDFYGGPPQIEPLLEYRGLVAPA"
     gene            3007..3387
                     /locus_tag="VNZ06_12010"
     CDS             3007..3387
                     /locus_tag="VNZ06_12010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21525.1"
                     /translation="MSNSTLTRTLKFTGFAASLALAVCLSLAPFATRADEKVTFHFAP
                     FSINDAQLFEFVASRLLSTEGAEQLDTLRDELMFTRLGPDSFMVMARGFYLAEPSHGV
                     FKRISPNWNEEAATVPVKLIARSP"
     gene            complement(3464..3964)
                     /locus_tag="VNZ06_12015"
     CDS             complement(3464..3964)
                     /locus_tag="VNZ06_12015"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HWW21526.1"
                     /translation="MKAQISLVVAVADNGVIGRDGKLPWRLPDDMKHFRNLTLGKPLL
                     MGRRTFESIGRPLSGRRNLVLSHTAMVPLAEVETVRSLEQALALIGEQELCVIGGAAV
                     FALALPSASCIYLTRVHGRPEGDTRFPLSALREWQEIERSEHPADERHGYAMSFVRLQ
                     RTAATG"
     gene            complement(3961..5850)
                     /locus_tag="VNZ06_12020"
     CDS             complement(3961..5850)
                     /locus_tag="VNZ06_12020"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWW21527.1"
                     /translation="MAAQAQGPQQVKNSRTTATCVLGAMGVVYGDIGTSPLYALESSL
                     TAAGVPTDRMAILGVLSLIFWSLAVVVTLKYVVLIMRADNEGEGGILSLFALVQRNLA
                     VGNRRQRVIVMLAALGAALFYCDALITPAISVLSAVEGLQVLSPDTAHAVVPVTIIIM
                     IVLFAIQHRGTERVGRMFGPIMLTWFLALSASGVSALVKNPSVLAALSPGYGVLLLTH
                     RPGVALAIIGGVFLAMTGGEALYADMGHFGKKPVRIAWFAFVWPALVLNYFGQGAMIL
                     MHPSASHLPLYGLFPESMLLPAVVLATLATIIASQATITGAFSVTRQCIQLDLLPRLR
                     IRQTSALEHSQIYVPVVNWFVLAAVCMFVLAFRSSDALGAAYGAAVAGTMFITTLLGA
                     QLALARWRWHYWQVLAVFLPLSLVDLMFLTGNMTKFAEGAWVPLTLAMILFAVFMIWR
                     SGRRLLRSALRAVAVPLQQLPKLLADVERVPGTAVFLASDPKHIPTALMRNLEHNHVA
                     HERIVFLNMEIARTPRQDPADRVRIETLMPEIYAVTARFGFMETPDVNEALKQCRARG
                     LKLFAQDCSFFLGWHLVAPRPRGGYEGVRRRLFAWMQRSSAQAAEFFRMPERRVIVLA
                     TQVEL"
     gene            complement(5817..6272)
                     /locus_tag="VNZ06_12025"
     CDS             complement(5817..6272)
                     /locus_tag="VNZ06_12025"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HWW21528.1"
                     /translation="MMRVRRSRVHGRGVFALRLIRKGTRIIEYLGDRITHRQADVRYE
                     DKNDRDSHTFLFIVDRGVVIDAGVNGNDARFINHSCEPNCESVIDDRRVFIEAIRTIR
                     AGDELCYDYQIGRDRSDPPDVDEIFACRCGAKACRGSMLWPPKRKARSK"
     gene            complement(6365..7159)
                     /gene="thyA"
                     /locus_tag="VNZ06_12030"
     CDS             complement(6365..7159)
                     /gene="thyA"
                     /locus_tag="VNZ06_12030"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HWW21529.1"
                     /translation="MKAYLELVRAIRTRGVRKDDRTGTGTLSLFGQQMRFDLSAGFPL
                     VTTKKLHLPAIVYELLWFLRGDTNVRYLREHGVTIWDEWADPNGELGPVYGKQWRSWG
                     TADARQIDQISEVVNQLRSNPNSRRILVSAWNVGELPQMALAPCHAMFQFYVAANRLS
                     CQLYQRSADVFLGVPFNIASYALLTCMLAQQCDLQLGEFVWTGGDCHLYLNHLEQADL
                     QLARSPLPMPQLKMRRRPASIFEYQYEDFEFMDYQFHPAIRAPVAV"
     gene            complement(7156..7974)
                     /gene="lgt"
                     /locus_tag="VNZ06_12035"
     CDS             complement(7156..7974)
                     /gene="lgt"
                     /locus_tag="VNZ06_12035"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HWW21530.1"
                     /translation="MLPYPNINPIALKLGPLQIHWYGIMYLLGFAGAWWLGRRRAAQP
                     NSSWSAVDVDDFLFYGMLGVIVGGRIGYVLFYGMPLWRADWLYPLKIWEGGMSFHGGL
                     LGVLAATAFFAWKHGRKVWDVVDFAAPWPGIGLFFGRLGNFINGELWGRPTEGWWAML
                     VPDPNGGQPVPRHPSQLYEATLEGIVLFTLVWIYSSRPRPRYAVSGLFAICYGVARIV
                     VEFVREPDIGIGYLAYGWVTMGQVLSLPLLLAGIFLMGLAYSRHEPSGNFKPPQ"
     gene            complement(7992..>8230)
                     /locus_tag="VNZ06_12040"
     CDS             complement(7992..>8230)
                     /locus_tag="VNZ06_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="RlmI/RlmK family 23S rRNA methyltransferase"
                     /protein_id="HWW21531.1"
                     /translation="LNQIAMRLLERDALLVSCSCSYHLSGDELLGLIQSAARHTSRFA
                     QVLHCGGQAQDHPIHPAIPETRYLKAFFCRVTRE"
BASE COUNT         1601 a         2627 c         2529 g         1473 t
ORIGIN      
        1 atcgatgctg ctcgcattcc tcggggtgct gctgctgcgc cgtgcgccac gtctcggacg
       61 cgcgctggtg gtgatcggga tcgcatcgat ctggttgctg tccaccccga ttgtcgccga
      121 ccagctcacg gtctgggtcg agcactatgc gccgctcgac ctgcgtcagc cgaccggcgc
      181 gcaggcgatt gtgatactgg gaggaggagg ccagcgcagg tacgcgccgg aatacgcggg
      241 gccggaagcc gagccctatc tgctggaacg ggtcgcgtac ggcgcctttg tcgcacagcg
      301 cacgggactg cctatcctgg tcaccggctt tgggattgaa gcgctggcca tgcgcgaatc
      361 actcaagcgt cacttcagca tcgaagcacg ttgggtggac gatcaatcct atgacacttt
      421 cgagaacgcg cggaactcgg tacggatcct gaagtccgca ggtattcaac gcacgattct
      481 ggtgacccgt tccacccacc tgtggcgctc ggtacacgag ttcgaggccg caggcattga
      541 ggtggttccg gctccggtag gcagtttcgc ggtgagagat cttaatattt tccgctttct
      601 cccggacaca gaggcgttga cgcgctcgca cgcggcgatc tatgaagccc tgggcgacct
      661 ggtgcggcgg gcgcttgcca cgacgcatct gcgccggcac tgacggttct gtcaggttct
      721 gttccgactg cgggatattg gttgtcacga tgatgagccc agacaatccg ttttttcatc
      781 cgagcgctct gccgtaccag ttcccgccct tcgaccgtat ccgaagtacc gacttcgtgc
      841 ccgcctacct ggccggaatc gagcagcacc ggcaggaggt tcaggcgatc tgcgccaatc
      901 cggccccggc aacgttcgac aacaccatcg tcgcgctgga gcgttcgggg cggctgctcg
      961 atcgggtgga cagcgcattt tcaaatctca gcagctcgga cggcgatgcg gcgatgctgc
     1021 agatcgaatc cgaaatggca ccgaggctgg cggcacacga agacgccatc tacctgctgc
     1081 cgcctctgtt tgcccgcatc gagcagctct acgcacaccg cgggcaactc aagctcgatg
     1141 ccgaatcgct gcagctgctc gagcgttatc gcgttcagtt cgttcgcgcg ggagctctgc
     1201 tgtccgagcg cgacaagagc cggctgcggg agctgaactc acgcctgtcc acgctgacca
     1261 cgcaattccg ccaaaacgtg ttgcgctcca gccgtgacag cgcggtgttg attgacgatg
     1321 aagcgcagct ggacgggctc gcgcccgatg agctcgcggc cgcggcggcc aacgctgcag
     1381 cgcggggcct gggcggcaaa tgggtgatcg ctctgcagaa caccacgacc caaccgctgc
     1441 tgtcgcagct cacgcgacgt gacgtgcgcg agcggatctt tcgcgtctcg acgcagcggg
     1501 cccgtggcgg cgaggctgac aataccggga tcatcgccga gatcgtgcga ctgcgtgccg
     1561 aacgcgcgcg cttgctcggc tgcccggatc atgcaaccta tgtgctgaag gatgagaccg
     1621 ccggcagctc cgcggcggtg gacgcgatgc tgcagcggat cgccgctgcc gccctgtact
     1681 acgcccgaca acaggccgcc gggatcgagc agctgatcgg cgcgcagagc acgttgcaac
     1741 cgtgggactg ggacttctat gctgcgcagg tccgcaagac gaaatacgat tttgacctct
     1801 cgcaggtcaa accctacttc gaactgaacc gcgtcctgca ggatggcgtg ttctttgtcg
     1861 ccagcgaatt gttcgggctg tcgttcgtcg agcgccacga ccttccggcc taccaccccg
     1921 acgtacgcgt gttcgaggtg ttcgaggccg acggctcggc gctgggactg tttgttgccg
     1981 atttctatgc gcgcgacaac aagcagggtg gcgcctggat gaaccacttc gcggttcaat
     2041 cgcagctgct gggacgcaag cccgttgtgg tcaatcagct caacataccg aaaccggcat
     2101 ccggcgcccc ctcgctactg acgttcgagg atgtgaccgc gatgttccac gagttcggtc
     2161 acgcgctgca tggcctgctc tccaacgtct gctttccatt gctgtccggc attcgcgtgc
     2221 cgcgcgactt cgttgaattc ccgtcgcaat acaatgaaat gtggacgcgg gaacccgcgg
     2281 tgctggccaa cttcgcgcgg cactatcaga ccggcgcggc gatgcccacg gcgctactgg
     2341 aaaaggtact ggcggcgcag aatttcgatc aagggcaccg cacctccgaa tatgtgcagt
     2401 cggcgatcat cgatcaggcc tggcaccggc tcagcgccga gcaaacaccc ccggccgctg
     2461 aggtcgtgca attcgaagcc gacatcctgc gacggcgcgg tgtcgcctac gaccctatcc
     2521 cgccgcgcta ccactcgtgg tatttccttc acatcttttc tggcggctac tcagcgggct
     2581 actacgcgta catctggagc gaagtgctgg ctcgcaatgt gggacgctgg ctgcatgagc
     2641 gcgggggact tacccgcgcc aacggacaat gcctgcggga gaagattctt tcccgcggac
     2701 gcacggtcga tccgatacgt ctgttcgagg atttctacgg cggtccgccg cagattgaac
     2761 cgctgctcga ataccgtggt ctggtcgcgc cggcttgagg acttgccata acaggtcgta
     2821 gcggcctgga ctcgttatgt acgcgctcga ccctaacgcg cgtgatgcac atcaccgttg
     2881 taaaagtccg ctggcgtctg tgatgcacga cagccattcg ccggttgcag aagagtaatt
     2941 tggcgccatc gttgtctgga agccacatcc tcaaccaact cttccgagtc aacgatggag
     3001 ggagtcatgt ccaactcgac attgacgcgt acattgaaat tcactggctt cgcagcctcg
     3061 ctcgcgcttg cagtttgcct gtcgctcgcg ccgttcgcga cccgtgctga cgagaaggtc
     3121 acgttccatt ttgcgccgtt ctccatcaac gacgcgcagc tctttgaatt cgtcgcttcg
     3181 cggctgctca gcaccgaagg cgccgagcag ctcgatacgt tgcgcgacga actgatgttc
     3241 acgcggctcg gaccggactc gtttatggtg atggctcgcg gcttctacct ggcagagccc
     3301 agtcacggtg tgttcaagcg catcagcccg aactggaacg aagaagctgc cacggttccg
     3361 gtgaagctca tcgcgcgtag tccgtaaaca tacaagcctt caggtttgtg ttggccgtgt
     3421 cggcgtggtc atggatggcc cgccgacatt gctatttcca acgctatcct gtcgccgccg
     3481 tgcgttgtag ccgcacaaag ctcatcgcat aaccatggcg ctcgtcggcc ggatgctcgg
     3541 agcgttcaat ctcctgccac tcacgcagag ctgacagcgg aaaacgggtg tcgccctcgg
     3601 gtctgccgtg cacccgggtg agatagatac aactggctga cggcagcgca agtgcaaaca
     3661 cggcggcgcc accgatcacg cacagctcct gctcgccgat cagcgccagc gcctgttcca
     3721 ggctacgcac agtctcgacc tcggcgagcg gcaccattgc agtgtggctc agcaccagat
     3781 tgcgccggcc ggacagcggc ctgccgatgg attcaaaggt gcgtcttccc atcagcagtg
     3841 gcttgccgag cgttaggttt ctgaagtgct tcatgtcgtc gggcagccgc cacggaagct
     3901 tgccgtcgcg gccaatcacg ccgttgtcgg caaccgccac taccagcgaa atctgcgctt
     3961 tcaaagctct acttgggtgg ccagaacaat cacgcgccgc tcaggcatgc ggaaaaactc
     4021 ggccgcctgc gcgctgcttc gctgcatcca ggcaaacagt cgccgccgca ccccttcgta
     4081 gccacctcgc ggccgcggcg ccaccagatg ccatccaagg aagaacgagc aatcctgcgc
     4141 aaacaatttc agcccgcgcg cgcggcattg tttgagcgcc tcgttcacat ccggggtttc
     4201 catgaagcca aagcgcgcgg tgaccgcgta gatctccggc atcagggtct cgatgcgcac
     4261 ccggtcggcc ggatcctgac gcggcgtgcg tgcaatctcc atgttgagaa atacgatgcg
     4321 ctcgtgcgcc acgtggttgt gttcgaggtt gcgcatcaac gccgtcggta tgtgcttcgg
     4381 atcgctagcc agaaagaccg cggtccccgg cacacgctcc acgtcggcca gcagctttgg
     4441 caattgctgt agcgggacgg ccacggcgcg caacgcactg cgcagcaggc ggcggccgct
     4501 gcgccagatc atgaagaccg cgaacaggat catcgccagt gtcagcggca cccacgcgcc
     4561 ctcggcgaac ttggtcatat tgccggtcag aaacatcaga tcgaccagcg ataacggcag
     4621 gaacacggcg agcacctgcc agtagtgcca gcgccaccgg gcgagcgcga gctgtgcacc
     4681 cagcaacgtg gtgatgaaca tggtgccggc aacggctgcg ccataggcgg cgccgagcgc
     4741 atcggaggag cggaaagcga gtacgaacat gcaaaccgcg gcgagcacga accagttcac
     4801 gaccggaacg tagatctggc tgtgttccaa tgcggaggtc tgccggatgc gaagccgtgg
     4861 cagcagatcg agctggatgc attggcgtgt taccgaaaag gccccggtga tggtggcctg
     4921 cgatgcgatg atggtagcca gtgtcgccag caccaccgcc ggcagcagca tgctctccgg
     4981 gaacagtccg tacagtggca agtgtgaggc agaggggtgc atcagaatca tcgcaccctg
     5041 gccgaagtag ttgagcacca gtgcgggcca gacgaacgcg aaccaggcga tgcgaaccgg
     5101 cttcttgccg aaatgtccca tgtcggcata cagcgcctcg ccaccggtca tcgccagaaa
     5161 cactccaccg atgatcgcca gtgccacgcc cggccgatgc gtcaacagca gcaccccgta
     5221 gccgggactg agtgccgcca gcaccgaggg gttcttgacc agcgcagaaa cgccggatgc
     5281 cgacagcgcc agaaaccagg tcagcatgat tgggccgaac atacgaccga cgcgctcagt
     5341 gccacgatgc tgaatcgcga acaacacaat catgatgatg atcgtcaccg gtaccaccgc
     5401 gtgcgccgtg tccgggctga gcacctgcag accttcgacc gcgctcagca ctgagatcgc
     5461 cggcgttatc agcgcgtcgc aatagaacag agcggcaccc agcgccgcga gcatcacgat
     5521 gacacgctgg cggcggttgc ctaccgcaag gttgcgctgt accagcgcga acagtgacag
     5581 aatgccgccc tcgccttcat tgtccgcacg catgatcagc accacgtact tgagcgtgac
     5641 cacgaccgcg aggctccaga aaatcagcga cagcaccccc agtatcgcca tgcgatcggt
     5701 cgggacgccg gcggcagtga gcgatgactc cagcgcgtac agcggactgg taccgatgtc
     5761 gccatagacg acacccatcg cgcccaatac gcaggtcgcg gtggtacgcg agttcttcac
     5821 ttgctgcggg ccttgcgctt gggcggccat agcatcgagc cgcgacaagc tttggccccg
     5881 cagcggcagg caaaaatctc gtcgacgtcc ggcgggtcgc tgcggtcgcg accgatctgg
     5941 tagtcgtaac acagctcgtc accggcccga atggtgcgga tcgcctcgat gaacacccgg
     6001 cgatcgtcga tcacggattc gcagttgggc tcgcaggagt gattgatgaa tcgcgcatcg
     6061 ttgccgttca cgccggcatc gatcacgaca ccgcgatcga cgatgaacag aaaggtgtgg
     6121 ctatcgcggt cgttcttgtc ctcgtaacgc acgtccgcct gcctgtgcgt gatgcgatcg
     6181 ccgagatact cgatgatccg cgtgcccttg cggatcagac gcagcgcgaa gacgccgcgt
     6241 ccgtgcacac gcgagcggcg gacacgcatc atcttgggtc ggctggcgat cctcgcaacg
     6301 atgcttttgc ggcgcttggc cggaagagtc ttgcgcgctg tgcgcttgtt ccggcgcgct
     6361 acgctcatac cgccacgggc gcgcggatcg ccggatgaaa ctgatagtcc atgaactcga
     6421 agtcctcgta ctggtactca aaaatgcttg cggggcggcg ccgcatcttc agttgtggca
     6481 tcggcagcgg gctgcgggcg agctgcaggt cggcctgctc cagatgattc agatagagat
     6541 gacagtcgcc accggtccag acgaattcac cgagctgcag gtcgcactgc tgggcgagca
     6601 tacaggtcag caatgcgtag ctggcaatgt tgaacggcac gccgaggaac acatcggcgc
     6661 tgcgctgata gagctgacag gacagccggt tggcagcgac atagaactgg aacatcgcgt
     6721 gacacggcgc gagcgccatc tgcggcagtt cgccgacgtt ccaggcgctg accaggatac
     6781 ggcgcgaatt cggattgctg cgcagctgat tgaccacttc actgatctga tcgatctggc
     6841 gagcgtcggc ggtgccccag ctgcgccact gtttgccgta caccgggccg agttcgccat
     6901 tcgggtcggc ccactcatcc cagatcgtca ccccatgctc gcgcaggtac cgcacgttgg
     6961 tgtcgccacg caggaaccac agcagctcat agacaatcgc cggcagatgc agtttcttcg
     7021 tggtcacgag cgggaatccg gcggacagat cgaagcgcat ctgctgcccg aagagtgaca
     7081 gcgtgccggt accggtgcga tcgtccttgc gcacaccgcg cgtgcggatc gcgcgcacca
     7141 gctcaaggta ggccttcatt gcggcggctt gaaattgccc gacggttcgt gacgcgagta
     7201 ggcgagtccc atcaggaaaa tcccggccag cagcaacggt agcgagagca cctgacccat
     7261 cgtgacccag ccatacgcca gatagccgat gccgatgtcc ggttcgcgca cgaactccac
     7321 tacgatgcgg gccactccat agcagatcgc gaacagcccg gacaccgcgt agcgcggtcg
     7381 aggacggctg ctgtagatcc acaccagggt gaacagcacg ataccctcca gggtggcctc
     7441 gtagagctgc gaggggtgtc gtggcaccgg ctgcccgcca ttcggatccg ggacgagcat
     7501 cgcccaccag ccttcggtcg ggcgccccca cagctcaccg ttgatgaaat tgccgaggcg
     7561 cccgaagaac agtccgatac cgggccacgg cgcggcgaaa tccaccacgt cccagacctt
     7621 ccgtccgtgc ttccaggcga agaacgccgt ggccgccagc acgccgagca ggccgccgtg
     7681 aaacgacatg ccgccttccc agatcttgag cggatagagc cagtccgcgc gccacagcgg
     7741 catgccatag aacagcacat agccgatgcg accgccaaca atgacaccga gcatgccgta
     7801 gaacagaaaa tcatcgacgt cgaccgcgct ccagctcgaa ttcggttgtg ccgctcggcg
     7861 gcggcccagc caccaggcac cggcaaaccc cagcaggtac atgatcccgt accagtggat
     7921 ctgcaacgga ccgagcttca gggctatcgg attgatattc ggatagggca gcatgcgcgg
     7981 attctaccgt gtcactcgcg cgtgactctg caaaagaaag ctttgagata gcgggtctcg
     8041 ggaatggccg gatggatcgg atgatcctgc gcctggcctc cacagtgcag aacctgggcg
     8101 aagcggctgg tgtgccgggc cgcagactgg atcaatccca gcaattcgtc acccgacagg
     8161 tgataggaac aggaacagct gaccagcagc gcgtcgcgct cgagcagtcg catcgcaatc
     8221 tgattgagct
//
LOCUS       DATMRM010000398         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_858787, whole genome shotgun sequence.
ACCESSION   DATMRM010000398 DATMRM010000000
VERSION     DATMRM010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858787"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="VNZ06_12045"
     CDS             <1..1521
                     /locus_tag="VNZ06_12045"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWW21532.1"
                     /translation="IEDNGYGISVKSELQTPGGNIAANLASFRKLQILDGNGSDLPSA
                     ATLMREAVMAARNDEGPVLLRLTVPRLSGHSGQDTQAYKSPQEVAEEQSRDPLSALHE
                     MLVPEHMTQDQWRQAEADARHEVLQALERVEARPTPDARQVTRFAFSEPRAGGGLELQ
                     QQGGIRSHGVQPAAGSDTVRAEGARINMLTAIRRTLEHELAVNSRVLVFGEDVGRKGG
                     VHAATLGLQERFGAQRVFDTSLSEEGIIGRAVGMALAGLMPVPEIQFRKYAEPAAEQL
                     NDCGTLRWRTANRFAAPMVVRMPGGFFKCGDPWHSQNNEVQFVHAIGWRVAMPSNAED
                     AVGLLRAALRGNDPTIFFEHRAMLDGAWARRPYPGDDYVLPFGRARCIRAGTEVTVIC
                     WGAMVERCEQAAAESRIDAEVLDLRTLVPWDREAVMRSVARTHRCLIVHEDNLSAGFG
                     AEIAASLARDAFYELDAPIERLAMPDVPSPHNPLLLEAVLPSVARIAQSLRDLASL"
     gene            1518..>2155
                     /locus_tag="VNZ06_12050"
     CDS             1518..>2155
                     /locus_tag="VNZ06_12050"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWW21533.1"
                     /translation="MSSTPGSIEVRAPRDQAEGTRSQILRWLKATGDALAEHEPLVEV
                     ETDKVTIEIASPASGVLREILKQEQEEISPGDLLARIEVSAAAASATAAVGASAGAGQ
                     STHAAAASAGSDAQLPARPRGRREPDSVSPAVRRLLQERGLSEQDVTGTGPGGRISVD
                     DVLRTDSAAPHASAPPSWAVSGAGTASHHVAHSATRKRIAQRMVHSLLHTAP"
BASE COUNT          359 a          694 c          779 g          323 t
ORIGIN      
        1 atcgaggaca acggctacgg catctcggtg aagtcggaac tgcagacgcc cggcggcaat
       61 atcgcggcga acctggcctc gtttcgcaaa ctgcagattc tggacggcaa cggcagtgat
      121 ctgccctcgg cggcgacgct gatgcgcgaa gcggtgatgg cggcacgcaa cgacgagggt
      181 ccggtgctgc tgcgactgac ggtgccgcga ctgtccggac actccggtca ggacacgcag
      241 gcctacaaat ccccgcagga agtcgcggaa gagcagtcgc gcgatccgtt gagcgcactg
      301 cacgaaatgc tggtgcccga gcacatgact caggatcagt ggcgtcaggc ggaagcggac
      361 gcacgccacg aggtgctgca ggcgctcgag cgtgtggaag cgcggccgac gcccgatgcg
      421 cggcaggtga cgcgctttgc tttcagcgag ccgcgtgccg gtggcgggct ggagctgcag
      481 cagcagggtg ggatccgctc acacggcgtg cagccggctg ccggttccga cacggttcgc
      541 gccgagggcg cgcgcatcaa catgctgaca gcgatccgtc gcacgctcga gcacgaactg
      601 gcagtcaatt cgcgcgtgct ggtgttcggt gaagacgtcg gccgcaaggg cggtgtgcac
      661 gcggcgaccc tggggttgca ggagcgcttc ggtgcacagc gcgtgttcga taccagtctg
      721 tcggaagagg gcatcatcgg ccgcgctgtc gggatggcgc tcgccgggct gatgcccgtg
      781 ccggagatcc agtttcgcaa gtacgccgag ccggccgcgg agcaactcaa cgattgcggt
      841 accctgcgct ggcggacggc gaatcgattt gccgctccga tggtggtgcg catgccaggc
      901 gggttcttca agtgcggcga tccctggcac agccagaaca atgaagtaca gttcgtgcac
      961 gcgatcggct ggcgtgtggc catgccgtcg aacgccgaag atgcagtcgg tctgctgcgt
     1021 gcggcgttgc gcggcaacga tccgacgata ttcttcgagc atcgcgcgat gctcgatggc
     1081 gcctgggcgc gccgccccta tcccggtgat gactacgtgc tgccgttcgg tcgtgcacgc
     1141 tgcatccgcg ccggcaccga agtcaccgtg atctgctggg gcgcgatggt cgagcgctgc
     1201 gagcaggccg cggccgagtc caggatcgat gccgaagtac tggatctgcg cacgctggtg
     1261 ccgtgggatc gcgaggcggt gatgcgctcg gtcgcccgga cgcatcgctg cctgatcgtg
     1321 cacgaagaca acctcagcgc cgggttcggc gccgagatcg cggcgagcct ggcgcgcgat
     1381 gcgttctatg agctcgatgc accgatcgag cgtctggcca tgcccgatgt acccagtccg
     1441 cacaatccgc tgctgctgga ggccgtattg ccgagcgtgg cgcgcatcgc gcagtcgctg
     1501 cgcgatctcg ccagcctgtg agctcaaccc ccggcagcat cgaggtgcgc gcgccgcgcg
     1561 accaggcgga gggcacgcgc tcgcagatcc tgcgctggct gaaagccacc ggcgatgcgc
     1621 tcgccgagca cgagccgctg gtcgaagtcg agaccgataa ggtcacgatc gagattgctt
     1681 cgcccgcgag cggtgtgctg cgtgagattc tcaagcagga gcaggaggag atcagcccgg
     1741 gcgatctgct ggcgcgcatc gaagtcagcg cggccgcggc gtcggcgacc gcggcagtgg
     1801 gcgcatcagc gggcgccggt caatcaaccc acgcggcagc ggcgagcgcc ggatcggatg
     1861 cgcagttgcc ggcgcggcca cgcggacggc gcgagccgga tagtgtcagt cccgcggtgc
     1921 gacgcctgct gcaagagcgt ggactgagcg agcaggacgt caccggcacc ggccccggcg
     1981 ggcgcatcag cgtggacgac gtgctgcgca cggacagcgc tgcgccacat gcgagcgcgc
     2041 caccctcatg ggctgtgtcc ggcgcgggca ctgcgagcca tcatgtggcg cacagcgcca
     2101 cccgcaagcg catcgcgcag cgcatggtgc acagcctgct gcacaccgcg ccgca
//
LOCUS       DATMRM010000399         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2087502, whole genome shotgun sequence.
ACCESSION   DATMRM010000399 DATMRM010000000
VERSION     DATMRM010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2087502"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..485)
                     /locus_tag="VNZ06_12055"
     CDS             complement(72..485)
                     /locus_tag="VNZ06_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW21534.1"
                     /translation="MSPRSLSVLLVEDSRVLAERLRETLLSVPGVQLAETVDSEADAV
                     AALQRHPLDVVLLDLHLRQGTGFGVLRAIPGSRAKDVVVIVLTNYDLAEYRRAAAALG
                     AQHFLDKLRDFDRLPSLLQQIGAGPGNSGTPESLN"
     gene            complement(610..1242)
                     /locus_tag="VNZ06_12060"
     CDS             complement(610..1242)
                     /locus_tag="VNZ06_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWW21535.1"
                     /translation="MSKVLLADDHAMVRAGLRQFLGDDHSITEIGEAASGNETLDQLR
                     TGSWDLVILDINMPDRSGLDILRYIRASYPDTKVLVLSGFPERQYAINVLKAGAGGYL
                     PKECAPEELLKATRAVMQGRRYVSANLAELLLSDLDGDTDQPLHRRLSEREFQIFCKL
                     AAGRAVSSIADELCLSVKTVSTYRTRILEKMSLTTNADITTYALRNGIIQ"
     gene            complement(1254..>2252)
                     /locus_tag="VNZ06_12065"
     CDS             complement(1254..>2252)
                     /locus_tag="VNZ06_12065"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWW21536.1"
                     /translation="AERQETGMTDATNIMVGRSPVIQAVRDLVERVAPTDATVLISGE
                     SGSGKELVAQAIHARSARSSGPFLGINCGALPPTLIESELFGFERGSFTGAQRNHAGM
                     VERASGGTLLLDEVTEMSADMQTRLLRFLETRRFFRVGGTQEIQTDVRVVAATNRSPV
                     QAVREGSLREDLLYRLAVFPIALAPLRERGEDVVLLADHFLLELNARNGTDKTFGPGA
                     QRKLREYHWPGNVRELKNAIERAYILCDRELELEPMNLHAVVAASERTVAHDEHGGVR
                     VPIGSSLAQAERWLIEATLAHWAGNKNRAAKMLGCSLKTLYNKLAAYERQATQAHA"
BASE COUNT          431 a          705 c          659 g          457 t
ORIGIN      
        1 ccgttgactg atatcctaaa gtccgcgcga ccgaaattca ttccggtcgc cgcaagcgtc
       61 tagggcaacc gctagtttag gctttcagga gtaccgctgt tgcctggacc ggcaccgatc
      121 tgctgcagca gtgacggcaa acggtcgaag tcccgcagtt tatccaggaa gtgctgggca
      181 cccagtgcgg cggctgcgcg gcgatactcc gccagatcgt aattggtgag tacgatcacg
      241 acgacgtcct tcgctctact gcccgggatc gcgcgcaaca cgccgaagcc ggtgccttgg
      301 cgcaggtgca aatcgagcaa cacgacatca agcggatgac gttgcagcgc agcgacggca
      361 tcggcttcgc tgtccacggt ctccgccaac tgtacgccgg gtacgctcaa cagggtctcg
      421 cgcagccgct ctgcaaggac ccgcgaatct tcgaccagaa gtaccgacag acttcgcggg
      481 ctcatctgag tcttcggtat gaaggagggt tgcacatcca tttcctggcc aatgtaaggc
      541 gagcgccgcg ttgaatgaaa cgtcgagatt tgaccgttcc gtgatcggag actgcctaca
      601 gcgacgccgt cattggataa ttccgtttcg cagagcgtag gtggtgatgt cggcgttggt
      661 cgtgaggctc attttttcca gtattcgagt gcgataggta ctcaccgtct tgacggacag
      721 acacagctcg tcggcgatgg aagagaccgc ccgaccggcg gcgagcttac agaatatctg
      781 gaattcgcgc tcggatagcc gacgatgcaa tggttgatcg gtgtcaccgt ccagatccga
      841 gagcagcaat tccgccagat tggcgctgac gtagcgtcgg ccctgcatca ctgctcgagt
      901 ggctttcagc agttcctcgg gtgcgcactc tttcggcaaa taaccgcccg ccccggcctt
      961 cagaacgttg atcgcatact gacgctccgg aaagccgctc aaaacaagca ctttggtgtc
     1021 cggatagctc gcgcggatgt aacgcagaat atcgagcccg ctgcgatcgg gcatattgat
     1081 gtcgaggata accagatccc agctgccggt gcgcaactga tccagcgtct cattgcccga
     1141 ggcagcctcg ccgatttccg tgatgctgtg atcgtccccg aggaactggc gaaggcccgc
     1201 ccgcaccatc gcgtggtcgt cagctagaag tacctttgac atgaatgcct cactcaggcg
     1261 tgagcttgcg tcgcctggcg ctcgtacgct gcaagcttgt tatacaacgt tttaagacta
     1321 catcccagca tcttggctgc gcggttcttg ttgccagccc aatgcgccaa cgtggcctca
     1381 atgagccagc gttcggcttg tgcgagtgac gatccgattg gaacacgcac tcccccatgc
     1441 tcgtcgtgag cgacggtgcg ctcgcttgct gccacaacgg catgcagatt catcggctcc
     1501 aattcaagtt cgcggtcaca caggatatag gcccgctcga tcgcgttttt gagctcgcgc
     1561 acattgcccg gccagtgata ttcgcgcagt ttgcgctgcg cgcccgggcc aaacgtcttg
     1621 tctgtgccgt tgcgggcatt tagctccagc agaaagtgat ccgcgagcag caccacgtcc
     1681 tcgccgcgct cgcgcagtgg agccagagca atcggaaaca ccgccaggcg atacaggaga
     1741 tcctcgcgca ggctgccctc acgtaccgcc tgcaccggcg agcgattggt cgccgccacc
     1801 acccgcacgt cggtctggat ctcttgggtg ccgccgacgc ggaagaagcg ccgcgtttcc
     1861 agaaaacgca gcaggcgcgt ttgcatgtcg gcgctcatct cggtcacttc atccagcagc
     1921 aacgtgccgc ccgaggcacg ttccaccatg ccggcgtggt tgcgctgagc gccggtaaag
     1981 ctgccgcgct cgaagccgaa cagttccgac tcgatcaggg tcggcggtag tgctccgcaa
     2041 ttgatgccca ggaacggtcc tgaactgcgc gcgctgcgcg cgtggatcgc ttgagccacc
     2101 agctctttgc ccgaaccgct ctcaccggat atcagaacgg tcgcgtcggt gggcgcgact
     2161 ctctccacca ggtcccgtac cgcctgaatc acgggcgaac ggccgaccat tatgttggtt
     2221 gcgtcagtca ttcctgtctc ctgtcgttcg gc
//
LOCUS       DATMRM010000400         7331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3623484, whole genome shotgun sequence.
ACCESSION   DATMRM010000400 DATMRM010000000
VERSION     DATMRM010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3623484"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1170
                     /locus_tag="VNZ06_12070"
     CDS             262..1170
                     /locus_tag="VNZ06_12070"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWW21537.1"
                     /translation="MKSRLKRVPSFMLYGESSAFATAERLHIEDIQARSRRYLWEIAP
                     HTHRGLYQCLYVAAGPAATHVDDNHAQLSGPALVILPPGCVHAFRFSADTEGHVLTIA
                     PEVLFEGSDAAARVSFEALFAVPHVLRFEARDALALRLHPLFQRLLEEYRAPEGHGSP
                     VCVWLARSILWLIGQELQRRHELDGGTHRAHQSLTRFQALIELHYPDHWPISRYARLI
                     SMSEGRLNRLCRAQREHSAFELLQQRLLREARRRLAYVGMSVAQVARELGFRDPAYFC
                     RFFKRHTGVSPSRFRQQHLKEAALAD"
     gene            complement(1167..1619)
                     /locus_tag="VNZ06_12075"
     CDS             complement(1167..1619)
                     /locus_tag="VNZ06_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWW21538.1"
                     /translation="MLKVRAAAPADLPVIYELIGELADYERLRSQMVGKIDDLRRHLF
                     EEPRFAHVLIAEWEGIAAGFALYFLNYSTFLCRPGLYLEDLFVRPVHRGRGLGLALLE
                     ELERRARELNCGRFEWSVLDWNKPAIEFYRRFGANPMDEWTLFRKDLL"
     gene            1798..3408
                     /locus_tag="VNZ06_12080"
     CDS             1798..3408
                     /locus_tag="VNZ06_12080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWW21539.1"
                     /translation="MEQPARHPSASPEERFDSHAVTVTTDSSPGASARPAPPVVTLSE
                     DPMLLEAISAATLDLVQVIVAPSADRFIDQVVATGGELALIDASAVPDDLAQFLVSIH
                     QQFPQLQLLLAGPGNVQHLIGSQITDGTVYRFVHKPASAQRLKLFVDAALRERQTRIT
                     AQILRSPFPAPAAAEPAVRDSGRPGWLMAAAGMVLLIAAAGAVIWYSSLSEPPVAKAQ
                     PPALAPAVVVPRPAPAPIATRAAAPPAARATHTASPGALTEAEQAAVDRAAAERSERS
                     EKERLAAEAEARQAAQAEQARRADSDARLAQIHQWVQLARSRIASGALIEPANDSART
                     YVSDAQEQAPDDQEVRAVSVALGDALINSFRKALAAGNTAAAEEWLKACRAYQISRAT
                     LDQMAVQLESFRAAQVAQSGATKAVENALAADALVTAPATPVAGPKPVQASTQILQEG
                     DLHRIDFNPPKYPPDALMRGETGTVELDFTVTPAGTVTDIKVTRANPIGVFEQASIAA
                     LVHNRYEPVLRDGAPVAQRAHIRMRFAL"
     gene            3405..4697
                     /locus_tag="VNZ06_12085"
     CDS             3405..4697
                     /locus_tag="VNZ06_12085"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWW21540.1"
                     /translation="MNLRLLIIGDERELRLWLHHQVDVLWPDAVVDEVEQAQLEARAG
                     TLRMRELQLVLLCVKCDPASDDPAADLEPLRSLLRHRDVPPTVVVTSGGNELTAVRAI
                     QLGANDYLPRELLSGQRLATALRSAMKAPRHAPPARTRARRSPRRKIDPAHLNLPQYS
                     ILHKLGESSRAAVYLAHSVSLGRNVALKISKPMMEDADECNEFAREYAAISALHHRSV
                     VEIYDYGFHDGREFISMEYFPCGDLKVRLQQPITARESLEFAHRICMALEVIHARGLI
                     HRDLKPPNVMLREDGSIVLIDFGIAKGVEIGGRSTAVGVLRGSPYYMSPEQAQGIPLD
                     ARSDLYSVGVILFEMLSGSKPYTGTSAMEVMQQHVHGPRPRLPIAQVEYEALLEHFMA
                     RQRDERFPDAISASGALSDALERLTREASPAPVAAHGG"
     gene            4699..5742
                     /locus_tag="VNZ06_12090"
     CDS             4699..5742
                     /locus_tag="VNZ06_12090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW21541.1"
                     /translation="MSDTAQQPQMIIPALQSELDSLRDQRDLYRSLLLCEPEPLAEFI
                     CVALETVETLRSTLRMPTRDSGAFRGKIERLQMELMSMEQALTGMHLPTVSLRLQTAV
                     AAVNEIGLRAEITGNDLLPAMVLLEELCSHVLIASNAAAVHVPIADEDEQEEELEASE
                     RRAQRKLGVALRQMCDKLSAEHGKKMSLVTIGFEEMPGDWSSVLFDLLGQLLRNSIEY
                     GIEPADARLAAGKPDVGTMAIEFVDRGTQGFELNVQDDGAGLDTDRITDVAVRLGLLP
                     PDCVRGLDPGRLLNLIFQPGVTTSQDPARRGLGMQIVREHVQRLGGKMQIAAKRGQYL
                     RFQISLPPLTESD"
     gene            complement(5749..6765)
                     /locus_tag="VNZ06_12095"
     CDS             complement(5749..6765)
                     /locus_tag="VNZ06_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HWW21542.1"
                     /translation="MNAAVGRLRPRGRVDLGDYPVDAAWSNDGKTLIVAGGEGAILRL
                     SSSGTDPAQVIGRHPSGVLAVSWQKAGRLFVTSGQDGAVLLWDARSLTPQKIQQAAQW
                     SQRLAFSDSGRWLAVASGKTLHLYDDSGKLQHRLDDHPGSIAAIGWRPKSHEVAAAGN
                     NGLRVHRLEPELQLRDFRARGACLTAAWHPEGRVLAAGMQDGTINLWNVATGTQSDIQ
                     GLGSRVFATEWSSNGRYLAAAASSALVGWDSQSKSSQSSQPFELRAHSDRLTALSFRP
                     SGTWLVSAARDRRLLLWRVGAAEHPQDAHLLPDEGTVLRFSRDGALLAVGDALGGLTI
                     YECS"
     gene            complement(6762..>7331)
                     /locus_tag="VNZ06_12100"
     CDS             complement(6762..>7331)
                     /locus_tag="VNZ06_12100"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWW21543.1"
                     /translation="AADGDAAALDSLLDSTGKIFQSDPELIAPGETIHAQRRSRLQLG
                     QHASFKLEISSSGPWALFTEHAPIEFGMHLHAPAPIVTRRFGSHHHDLAIGSIGLTDE
                     RELDPNKVNQWLSYLLQSRGQDILRMKGVLNLKAQPRRFVFHGVHMMFDGQLERPWGA
                     GTPRVSRLVFIGRDLDRNELEAGFESCVA"
BASE COUNT         1361 a         2364 c         2348 g         1258 t
ORIGIN      
        1 cggatgcgct tcgctgccaa tcagcagcag ctcgccgtca tagccctcct ggcgcagcga
       61 ttccgcgatc tgcactccgg cctgcccggc gcccactatc agaacaggat gcgacatggg
      121 ttgaaggata cccgactacg aaccatcacc cgcgacaaac cgcgtgccac ttgtactttt
      181 gtggtgcgag agcccatccg cacccagtgt tatgatagta gccctgcgtc tcggcaaccg
      241 gtagcttatg tggaaatgca catgaaatcg cggctcaagc gcgtacccag cttcatgctg
      301 tacggcgaat cgagtgcctt tgcgaccgca gaacgcttgc acattgagga cattcaggcg
      361 cgcagccgac gctacctgtg ggagatcgcc ccgcacaccc atcgcggact gtatcaatgc
      421 ctgtatgtgg ccgcaggacc cgcggccacg catgtcgatg acaatcacgc gcagctgagt
      481 ggtccagccc tggtgatcct gccgccgggc tgcgtgcacg ccttcaggtt cagcgccgac
      541 accgaagggc atgtgctcac gatcgcaccc gaggtgctgt tcgagggcag cgatgcggcc
      601 gcgcgcgtat cgttcgaagc gctattcgcg gtaccgcatg tattgcgctt cgaagcgcgc
      661 gatgcactgg ccctgcgcct gcatccactg ttccagcggc tactggagga atatcgtgct
      721 cccgaaggcc acggctcacc ggtgtgcgtg tggctcgcgc gcagcattct gtggctcatc
      781 ggacaggagc tgcagcgccg tcacgaactc gacggcggca cgcaccgggc gcatcagagc
      841 ctcacccgtt ttcaggcgct gatcgagttg cactatcctg atcactggcc gatctcgcgt
      901 tacgcgcgcc tgatcagcat gagtgaggga cgcctgaacc ggttatgccg ggcacagcgc
      961 gaacattcag ccttcgaact gctgcagcaa cggctgttgc gcgaggcgag acgtcgtctg
     1021 gcgtacgtgg gtatgtcggt cgcacaggtg gcgcgggaac tcggctttcg cgacccggct
     1081 tatttttgcc gcttcttcaa acgccacacc ggcgtgtcgc ccagccggtt tcgccagcag
     1141 cacctcaagg aagcggcgct ggcagactag agcagatcct tgcgaaacag ggtccactca
     1201 tccatcgggt tggcaccgaa gcgacgatag aactcgatcg ccggtttgtt ccaatccagt
     1261 accgaccact cgaatcggcc gcaattgagc tcgcgggccc gccgctcgag ttcttccagc
     1321 agcgcaagac ccagaccccg gccgcgatgc accggtcgca cgaacaggtc ttccagatac
     1381 aggcccggtc ggcacaggaa tgtcgaatag ttcagaaagt acagcgcgaa tccggccgcg
     1441 atcccctccc attccgcgat cagcacgtgc gcgaagcgcg gctcctcaaa cagatgacgg
     1501 cgcaagtcat cgattttgcc caccatttgc gaccgtagcc gctcgtagtc cgccagttca
     1561 ccgatcagct cataaatgac aggcaggtcc gcaggcgcag ccgctcgcac tttcagcatc
     1621 ttcgctctcc gcaacaggtg aggcagttca cagtatcggc gggcgggcat cggtcaaagt
     1681 cagattttgt taaggcctgc gaccgggcca cattctcggc gtggacagag tcattagagt
     1741 ctgcaggatc ggacgacgct ctgcccgaca cgattgattt taagccacgg aagaaggatg
     1801 gaacagccag cgcgacaccc ctctgcttca cctgaagagc gattcgattc ccatgccgtc
     1861 acggtaacga ctgacagttc gccgggagca tcggcgcgcc cggcaccgcc ggtcgtgacg
     1921 ctgtcggaag atccgatgct gctcgaggcg atatcggcag cgaccctgga tctcgttcag
     1981 gtcatcgtgg cgccatcggc ggatcgcttc atcgaccagg tggtggccac tggcggcgag
     2041 ctggcgctga tcgatgcatc cgcggtaccg gacgatctgg cgcagttcct ggtgtcgata
     2101 caccagcagt ttccgcagct gcagctgcta ctggccggac ccggaaacgt gcagcatctg
     2161 atcggctcgc agataacgga cggcacggtg taccgtttcg tccacaagcc agcctcggcc
     2221 cagcgactga aactgttcgt ggacgcggcg ctgcgcgagc gtcagacgcg gatcaccgcg
     2281 cagatcctgc gttcgccgtt tccggcgcct gcggcggccg agcctgctgt gcgcgactca
     2341 ggacgccccg ggtggctgat ggccgctgca ggcatggtgt tgctgattgc agccgcgggc
     2401 gccgtgatct ggtattcatc gttgtcggaa cctccggtgg cgaaagcgca gcccccggcg
     2461 ctcgctccgg cggttgtggt gccacgcccg gcgccagcac cgatcgcaac acgcgcggcg
     2521 gcacccccgg ccgcgcgagc cacgcataca gccagtcctg gcgcgctcac cgaagccgag
     2581 caggccgcgg tcgatcgggc tgcggcagag cgctccgagc gctcggaaaa agagcgcctg
     2641 gccgcagagg ccgaggcacg ccaggcggcc caggccgaac aggcgcgccg ggctgacagc
     2701 gacgcgcgcc tggcgcagat ccatcaatgg gtgcagctgg cgcgcagtcg cattgccagt
     2761 ggcgcgctga ttgaaccggc gaatgatagt gcacgcactt acgtcagtga tgcgcaggaa
     2821 caggcaccgg atgaccagga agtgcgcgct gtgtcggtgg ccctgggtga cgcgttgatc
     2881 aattccttcc gcaaagcgct ggcagccggc aatactgcgg ctgctgagga gtggctcaag
     2941 gcatgccgcg cctatcagat cagccgggcg acactcgatc agatggcggt gcagctcgag
     3001 agcttccgcg ctgcacaggt ggcgcaaagt ggagcgacga aagccgtgga gaatgcgctc
     3061 gcggccgacg cgctggtgac agcgccagcc acgccggttg ccgggccgaa accggttcag
     3121 gcgagcacac agatcctgca ggagggtgac ctgcaccgca tcgatttcaa cccgcccaag
     3181 tatccgccgg acgccctgat gcgcggcgaa accggcaccg tcgaactgga cttcaccgtc
     3241 acgcccgcgg gcaccgtcac tgacatcaag gtcacgaggg ccaatccgat cggggtgttc
     3301 gagcaggcgt cgattgcggc gttggtacac aaccgctacg agccggtact acgcgatggt
     3361 gcaccggtcg cgcagcgcgc tcacattcgg atgaggtttg cgttgtgaac ttgcgcctgc
     3421 tgatcatagg cgacgagcgc gaactgcgcc tgtggctgca tcatcaggtc gacgtgctct
     3481 ggcccgatgc ggtggtggat gaggtggagc aggcgcagct cgaagcacgc gctggaacgc
     3541 tgcggatgcg cgagctgcaa ctggttctgc tatgcgtgaa gtgcgatccg gcgtccgacg
     3601 atccggccgc tgacctcgaa cccctgcgca gtctgctgcg acatcgcgat gtaccgccga
     3661 ccgtcgtcgt gacctccggc ggcaacgagc tgacggcagt gcgcgccata caactgggcg
     3721 ccaatgacta cctgccgcgc gaattgctca gtggtcagcg cctggcgacc gcgctgcgca
     3781 gcgcgatgaa agcgccccgc cacgcgccac cggcgcgcac gcgcgcgcgc aggtcgccgc
     3841 gacgcaagat cgatccggct cacttgaatc tgccgcagta ctcgatcctg cacaagctcg
     3901 gagagtcctc gcgcgcagcg gtctacctgg cgcacagcgt gagcctgggg cgcaacgtgg
     3961 cgctcaagat cagcaagccg atgatggagg acgcggacga gtgcaacgag ttcgcgcgtg
     4021 agtacgccgc catttcggcg ctgcatcatc ggtcggtagt cgagatctac gactacggct
     4081 ttcatgacgg gcgtgaattc atctcgatgg aatattttcc gtgcggcgac ctgaaggtca
     4141 ggttgcaaca gccgatcacc gcgcgtgaga gcctggaatt tgctcaccgc atttgcatgg
     4201 cgctggaggt cattcatgcc cgtggactga tccaccgcga tctgaaacca ccgaacgtga
     4261 tgttgcgcga ggacggctcg atcgtgctga tcgactttgg catcgccaag ggtgtcgaaa
     4321 tcggcgggcg cagcacggcg gtgggcgtac tgcggggttc gccctactac atgagcccgg
     4381 aacaggcaca aggtatcccg ttggacgcgc gcagcgacct ctacagcgta ggcgtgatcc
     4441 tgttcgagat gctcagcggg agcaaaccct ataccggtac cagtgcgatg gaggtcatgc
     4501 aacagcacgt gcacggaccc cggccacggc tgccgatcgc gcaggtagag tacgaggcac
     4561 tgctcgagca tttcatggcc cgtcaacgcg atgagcgttt cccggatgcg atctcggctt
     4621 cgggcgcgct cagcgacgcg ctcgaacggc tgacgcgcga agcgagcccg gcgccggtcg
     4681 cagcgcacgg tggctgagat gagcgacacg gcgcagcaac cccagatgat cattcccgca
     4741 ctacaaagcg aactcgactc gctgcgcgat caacgcgatc tgtatcgctc gctgctgctg
     4801 tgcgaacccg agccgctggc cgaattcatc tgtgtggcac tggaaacggt cgagaccctg
     4861 cgctcgacgc tgcggatgcc gacccgcgac agcggcgcgt ttcgcggcaa gatcgagcgc
     4921 ctgcaaatgg aattgatgtc gatggaacag gccctcacgg gcatgcattt gcccaccgtc
     4981 agcctgcgcc tgcagaccgc cgtggcggcc gtcaatgaaa tcggcctgcg cgccgagatc
     5041 accggcaacg acctgctccc ggccatggtt ttgctggagg agctgtgtag ccacgtgctg
     5101 atcgcgtcga atgccgcggc ggtacacgta ccgatcgccg acgaggacga gcaggaagag
     5161 gaactggagg cgagcgagcg gcgggcacag cgcaaactcg gggtcgcctt gcgccagatg
     5221 tgcgacaagt tgtcggccga gcacggcaag aaaatgagcc tggtcacgat cggatttgaa
     5281 gagatgccag gggactggag cagcgtgctg ttcgatctgc tgggtcaact gctgcgcaat
     5341 tccatcgagt atgggatcga gcctgcggat gcgcgcctcg ccgccgggaa gccggatgtc
     5401 ggaacgatgg ccatcgaatt cgtcgatcgc ggcacacagg gattcgagct caacgttcag
     5461 gacgatggag cgggactcga caccgatcgc atcaccgacg ttgccgtcag acttgggctg
     5521 ctgcccccag actgcgtgcg tggtctggat ccgggacgtc tgctgaatct gatctttcag
     5581 ccgggtgtca cgacgtcgca ggatccagcg cggcgagggc tcgggatgca gattgtccgc
     5641 gagcacgtgc agcggcttgg cggcaagatg cagattgccg ccaagcgcgg tcagtacctg
     5701 cgcttccaga tttcattgcc gccgcttacc gagtccgact gacactggtc acgagcactc
     5761 atagatcgtc agtccgccga gcgcatcgcc gacagccagc aacgcgccgt cgcgcgagaa
     5821 gcgcagcacc gtgccctcat ccggcaacag atgcgcatcc tgagggtgct cggcggcgcc
     5881 gacccgccac aacagcagcc ggcggtcgcg cgcggccgag accagccagg tgccgctggg
     5941 tcggaaactc agcgcagtca ggcggtccga gtgggctcgc agctcaaacg gttgcgagct
     6001 ctgcgaagac ttgctttgcg agtcccagcc gaccagcgcg ctactggctg ccgcggccag
     6061 atagcgacca ttgctgctcc actcggtcgc aaatacacgc gatcccagcc cctggatgtc
     6121 ggattgcgtg cccgtggcga cgttccacag attgatcgtg ccgtcctgca ttcctgctgc
     6181 gagcactctg ccctcgggat gccaggcggc agtcaggcac gcaccgcgcg cacggaagtc
     6241 gcgcaactgc agctcaggct cgagtcgatg aacgcgcaga ccgttgttgc ccgcggccgc
     6301 gacctcatga ctcttcggtc gccagccgat cgcggcgatc gagccgggat gatcgtcgag
     6361 ccgatgctgc aacttcccac tgtcgtcata caggtgcagc gtctttccgc tggcgacagc
     6421 gagccagcga ccgctgtccg agaacgcgag tcgttggctc cactgcgcgg cctgctgaat
     6481 cttctgcggg gtgagcgagc gagcgtccca cagcaacacc gcgccgtcct gacctgaggt
     6541 gacaaacagt cgtcccgcct tctgccagga cacagccagt acgccggatg ggtgacgacc
     6601 gatcacttgc gcaggatcgg tcccactgct cgaaaggcgc agtatggcgc cctcgccacc
     6661 cgcaacgatc aacgtcttgc cgtcattcga ccaagcggca tcgacgggat agtcgccgag
     6721 atccacccgc ccgcgcggtc gcagtcttcc gaccgccgcg ttcatgccac gcaggattcg
     6781 aagccggctt cgagttcgtt gcggtctaga tcgcgaccga tgaacaccag ccggctgaca
     6841 cgcggcgtgc ccgcacccca gggccgttcc agctgcccgt cgaacatcat gtgcacgcca
     6901 tggaacacga agcgcctggg ctgcgccttg aggttcagca cgcccttcat gcgcaggatg
     6961 tcctgtccgc ggctctgcag caggtaagag agccactgat tgaccttgtt cgggtcgagt
     7021 tcccgctcat cggtcagccc gatcgagccg atggcgagat cgtgatgatg cgagccgaag
     7081 cgacgcgtga cgatgggcgc cggggcgtgc aggtgcatgc cgaactcgat cggtgcgtgt
     7141 tccgtgaaca gcgcccacgg cccggacgag gagatctcca gtttgaagct ggcgtgctga
     7201 cccagttgca gccgggatcg tcgctgcgcg tgtatcgtct ctcccggcgc gatgagttcc
     7261 gggtcggact ggaaaatctt tccggtcgag tccagcaggc tgtccaacgc tgccgcgtcg
     7321 ccgtcggctg c
//
LOCUS       DATMRM010000401         9299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3311612, whole genome shotgun sequence.
ACCESSION   DATMRM010000401 DATMRM010000000
VERSION     DATMRM010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9299
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3311612"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VNZ06_12105"
     CDS             <1..271
                     /locus_tag="VNZ06_12105"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWW21544.1"
                     /translation="QLTRRNTHSEYFLSIQVSYPNADDVRRAHSHHMDPGGSIMIHGL
                     PNNLRHSPDYYATADWTDGCIALSNSDMVEVWLMTQDNVRVEILP"
     gene            340..1725
                     /gene="ppnN"
                     /locus_tag="VNZ06_12110"
     CDS             340..1725
                     /gene="ppnN"
                     /locus_tag="VNZ06_12110"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF038390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HWW21545.1"
                     /translation="MQTSIVLPESVDARVGPRGSLEILSQAEIDKLLDTSQTGLHALF
                     RNCALAVLNSGSDNDNAKALFERYRNFDLRIVRHAWGIKLEMTNAPPAAFVDGEMIRG
                     IKEHLFAVLRDIIYTANEIIGAGRLDSRDSASITNAIFHILRNARLLDFNTRPNLVVC
                     WGGHSIGPVEYDYAKRVGYELGLRGLDVCTGCGPGAMKAPMKGAGVGHAKQRITQGRF
                     VGVTEPGIIAAEPPNAIVNQLVILPDIEKRLEAFLRLAHGIVVFPGGAGTAEEILYLL
                     GVLLEPANAGQPLPVILTGPRASAAYFDQITRFIERTLGPAALTRLEVIVDDAAGVAR
                     ALVKAFKQVRAFRREQNDSYTFNWLLRVPYELQQPFEVTHASMRALALQRYQPLHRLA
                     ANLRRAFSGIVTGNVKEYGVAAIERFGPYELSGDADLMALLDELLTAFISQGRMKLPG
                     SVYRPCYRLVA"
     gene            1924..2148
                     /locus_tag="VNZ06_12115"
     CDS             1924..2148
                     /locus_tag="VNZ06_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21546.1"
                     /translation="MSKPEAPKVETADEALAQSLTAPSGAEAYARWLEHMQRTRTRHS
                     AITRNLYTWSNYKNWAEKVRHDWLAEDTKP"
     gene            complement(2145..2885)
                     /locus_tag="VNZ06_12120"
     CDS             complement(2145..2885)
                     /locus_tag="VNZ06_12120"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWW21547.1"
                     /translation="MQRILVLNPKGGSGKTTVAINLASHFALRGERPVLMDYDKQGSA
                     GHWIKKRRPTQPHINLISAFENSLRVTRSFQVRIPQDTGRVVIDTPAAVSAQDMPELT
                     RTADKILVPVLPSDIDIHACSRCVQNLLLIAKVQREDNRLGIIANRVRRNTLIYRSLT
                     RFLGTLGIPIVATLRDSQNYVRAAEQGLGLEEMKAAQVSEDLAQWQPLLQWLDQPRGA
                     VAPVVPPPPAVEVPAIAEPERSAEPVLA"
     gene            3249..5150
                     /gene="htpG"
                     /locus_tag="VNZ06_12125"
     CDS             3249..5150
                     /gene="htpG"
                     /locus_tag="VNZ06_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HWW21548.1"
                     /translation="MSSERETLGFQAEVRELLHLMIHSLYSHREIFLRELISNASDAN
                     DKLRFLALSDPQMLGKESELGIWIDTDAKAGTITVRDNGVGMSREDAVVHLGTIAKSG
                     TREFFKRLTGDQQKDAQLIGQFGVGFYSAFIVADKVEVLTRRADTPAEAGVRWESSAD
                     GQFTVESVNRTERGTSVTLHLKEDAKEFSDPWRLRSLVRRYSDHIAFPVRMSKEGAAT
                     LEYEEVNQAQALWTRARSEIKDEEYRDLYRHISHDGADPLAWSHNRVEGKREYTSLLY
                     IPSRAPFDLWQREAARGLKLYVRRVFIMDDAEQFLPLYLRFVRGIVDSNDLPLNISRE
                     LLQQDREVEAMRSAITRRALELLARLAKEETDKYAQIWREFGRVLKEGLSEDPTNRDR
                     LLPLLRYASTHQEANEENVSLSDYVARMREGQERIYYIVAETVDAARASPHLELLKQK
                     GIEVLLMGDRVDDWVMGQISEFEGKKFKDVSRGDLELGGLADQAERARIDEERKEHKA
                     LLKRFKDALGDRVLEVRVSERLRDSPACLVHGEGEISAQMRRILSASGQSLPAGKPAL
                     EVNVAHPLVRHMDGIAQEPAFQELALLLYDQATLTDEGQVANPADFSRRLNNLLVRLV
                     AGSAQPAAP"
     gene            5189..5590
                     /gene="msrB"
                     /locus_tag="VNZ06_12130"
     CDS             5189..5590
                     /gene="msrB"
                     /locus_tag="VNZ06_12130"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HWW21549.1"
                     /translation="MGTETPTDAELKARLTPLQYQVTQKKGTEPAFTGKYAYNKEKGL
                     YNCVCCGQELFSSDTKYDSGSGWPSFWLPLASDRVRTRIDTSHGMERTEVTCSRCGAH
                     LGHVFDDGPQPSGLRYCINSASLGFDSVKKP"
     gene            5587..6234
                     /locus_tag="VNZ06_12135"
     CDS             5587..6234
                     /locus_tag="VNZ06_12135"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWW21550.1"
                     /translation="MSFRPPKAQALSIRGPAGALESLLEDPQAPVAAGFAVICHPHPL
                     FGGTMQNKVVHTLGRACQEFGLATLRFNFRGVGASDGSHDEGRAEIEDAMAVIAWGRQ
                     RWPDGPLTLAGFSFGAMVALAAAVRSAPSKLITVAPAVGRPEFSSAAKPNCPWLIVQG
                     DADELVDIRDVRAFAARFEPAPKLVVMSGVEHFFHGRLTQLRDEVLAFLKNEEAR"
     gene            complement(6296..6607)
                     /locus_tag="VNZ06_12140"
     CDS             complement(6296..6607)
                     /locus_tag="VNZ06_12140"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWW21551.1"
                     /translation="MAKKGGAPTKSEILAQISKDSELSRKQVSEVFESLNGIIKKSLR
                     GNGLFTLPGLLKLKVVKRPATKAREGINPFTGEKMTFKAKPASKKVRALPLKNLKSMV
                     N"
     gene            complement(6789..7316)
                     /locus_tag="VNZ06_12145"
     CDS             complement(6789..7316)
                     /locus_tag="VNZ06_12145"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HWW21552.1"
                     /translation="MDALIYLVDTLLSLCLLAVLLRLLLQWSRADFRNPLARSLVHIT
                     NPIIVPLRRLLPAIGRLDTASCVAVIVFALLEVAAAPLLKGFGPLPVLLWLRFAAIEI
                     VRTTLWTYFFAIVVYALLSFVAPGTYSPAQGLLVALCEPVLRPFRRVIPPLGGVDFSP
                     MWAGILIQMLLILVH"
     gene            complement(7317..8168)
                     /gene="proC"
                     /locus_tag="VNZ06_12150"
     CDS             complement(7317..8168)
                     /gene="proC"
                     /locus_tag="VNZ06_12150"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HWW21553.1"
                     /translation="MQQSVAVRIAFIGGGNMARALIGGLKRHAVPAEHISVGEPDAQA
                     RAGLQRDFGIRVSADNQEMLDGAQLVVLAVKPQQAGHILQALRPALQASRPLLFSIAA
                     GLRIADLRTACGPGIAIVRAMPNRPALLGAGVTGLFAAPGISAEQHRDAELIGQASGQ
                     AVWLRSEDELDVVTALSGAGPAYFFLLAEKLAHAAEGLGLTRDTALQLARATLHGAGM
                     LANEGVGLVEERAAVTSKGGTTEAAVRVLEQGGFTQLVASALQAGVARSAELANSLSA
                     TTSPERK"
     gene            complement(8180..8908)
                     /locus_tag="VNZ06_12155"
     CDS             complement(8180..8908)
                     /locus_tag="VNZ06_12155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HWW21554.1"
                     /translation="MLIAPHNSAADTLISGNLQQLRAQVAEAAGAARRTVESITVIAV
                     SKGHEASSVRAAAVSGVLHFGESYLQEATRKLESLRDLQASWHFIGRLQANKSRSVAE
                     SFDWVHGIDRLKIAQRLSAQRPHYAPALQVCLQVNIAGESGKAGVAPERVSELARAVA
                     LLPRLQLRGLMCILPAQLSSADNRRHFGALRRLQEQLNAQGAGLDTLSMGMSADFRDA
                     ILEGATLIRVGTAIFGPRTASSDE"
     gene            8980..>9299
                     /locus_tag="VNZ06_12160"
     CDS             8980..>9299
                     /locus_tag="VNZ06_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein PilT"
                     /protein_id="HWW21555.1"
                     /translation="MAVDIAQLLAFAVKNNASDLHLSAGVPPMIRVDGDMKRINMPAL
                     THKEVHGMVYDIMNDKQRKAYEEFFETDFSFEIPKLARFRVNAFNQNRGAGAVFRNIP
                     SAIL"
BASE COUNT         1794 a         2891 c         2905 g         1709 t
ORIGIN      
        1 ccagctcact cgccgcaata cccatagcga atacttcctg tcgatccagg tgtcctatcc
       61 gaatgccgac gacgtacggc gcgcgcacag ccatcacatg gatcccggcg gctccatcat
      121 gatccacggg ctgccgaaca acctgcgtca ttcgcctgac tactacgcca ctgccgactg
      181 gaccgacggc tgcatagcac tgtcgaattc ggatatggtg gaggtatggc tcatgacgca
      241 ggacaacgtg cgggtcgaaa tcctccccta agacttatcc ttccgcgctg tggcggcgtg
      301 atgcgcccgc gcgcccgccg ccactgaaag cttcccttca tgcaaaccag tatcgttctc
      361 ccggaatcgg tcgatgcacg cgtcggtccg cgcggcagtc tggagatcct gtcgcaagcc
      421 gagatcgaca agttgctcga taccagccag accggcctgc acgcgctgtt tcgcaactgc
      481 gcactggcgg tgctcaattc gggcagcgac aacgacaatg ccaaggcgct gttcgagcgt
      541 tatcgcaact tcgatctgcg gatcgtgcgt cacgcctggg gaatcaaact ggaaatgacc
      601 aacgcgccgc ccgcggcttt cgtggacggc gagatgattc gcggcatcaa ggagcatctg
      661 ttcgcggtgc tgcgggacat catctacacc gcgaacgaga tcatcggcgc cgggcggctc
      721 gacagccgtg acagcgcttc gattaccaac gcgatctttc atatcctgcg aaatgccaga
      781 ctgctcgact tcaacacccg gccaaacctg gtggtgtgct ggggagggca ttcgatcggg
      841 ccggttgaat atgactacgc caaacgcgtg ggctatgagc tgggcctgcg agggctggat
      901 gtctgcactg gctgcgggcc cggcgcgatg aaagcgccga tgaaaggtgc cggcgtcgga
      961 cacgccaagc aacgcatcac gcaggggcgc ttcgtcggcg tcaccgaacc cgggatcatt
     1021 gccgccgagc ctcccaacgc catcgtcaat cagctcgtga tcctgcccga tatcgagaag
     1081 cgcctggagg cgttcctgcg cctggcgcac ggcatcgtgg tgttcccggg cggcgcgggc
     1141 acggccgagg agatcctgta tctgctcggg gtgctgctcg agccggccaa tgccggtcag
     1201 ccgctgccgg tgattcttac cgggccgcgc gccagtgccg cgtacttcga tcagattacc
     1261 cggttcatcg agcgtacgct gggcccggcg gcgctgacgc ggctcgaagt catcgttgat
     1321 gacgccgcgg gcgtcgcgcg agcgctcgtc aaggcgttca aacaggtgcg cgcgttccgc
     1381 cgagagcaga acgattcgta cacgttcaac tggctgctgc gcgtgccgta cgagctgcag
     1441 caaccgttcg aagtcaccca cgcatcgatg cgcgcgctgg cactgcagcg ctatcagccg
     1501 ctgcatcgac tggcagcgaa tctgcggcgt gcgttctcgg gaattgtgac aggtaacgtg
     1561 aaggagtatg gcgtcgcggc gatcgagcgt ttcgggcctt acgaactgtc gggcgatgcc
     1621 gatttgatgg ctctgctcga tgaattgctc actgctttca tttcccaggg gcggatgaaa
     1681 ttgccgggca gtgtgtaccg accttgctat cggctcgttg cctgaagata ttttttctac
     1741 tgttaagtga cgcgctgtgt ccgctcgcat cggcggattt aacgtttgcg tcggtaagct
     1801 accgtgcgcg tcatactcgc atcagagtgc gaagtgcgag cggcaccgtt cagaaccgct
     1861 gtgatccagt tcctgtcgag tgccgctgca gcctcgtagg cttcgcgacc aaggagccac
     1921 accatgagca aacccgaagc accgaaggtt gagactgctg acgaggcgct ggcgcaatcg
     1981 ctgaccgcgc cgagcggtgc cgaggcttac gcccgctggc tggagcacat gcagcgcaca
     2041 cgcacgcggc actccgccat cacgcgcaat ctgtatacct ggtcgaacta caagaactgg
     2101 gcggagaagg tccgccacga ctggctggcg gaagacacca agccctaggc caacaccggt
     2161 tccgcgctgc gctccggctc agcgatcgca ggcacttcca ccgcaggcgg tgggggcacg
     2221 accggggcga cggcgcctcg gggctgatcc agccactgca gcagcggctg ccactgcgcc
     2281 agatcttcgg acacctgggc agccttcatc tcctccagac ccagcccctg ctcggcggcc
     2341 cgcacgtaat tctgcgaatc gcgcagggtc gcgacgattg gaatgccaag ggttcccaga
     2401 aaacgggtca acgagcggta aatcagtgta ttgcgccgca cgcggttggc gatgatcccc
     2461 aggcggttgt cttcgcgctg caccttggca atcagcagca gattctggac gcagcgcgag
     2521 caggcgtgga tgtcgatatc cgacggcagc accggtacca ggatcttgtc ggcagttcgc
     2581 gtcagttccg gcatgtcctg cgcggaaacg gccgccgggg tatcgatcac gacccgaccc
     2641 gtatcctgcg ggatccgcac ctgaaacgag cgcgtcacgc gcaggctgtt ttcgaaggcc
     2701 gagatcaggt tgatatgcgg ctgcgtgggc cgacgtttct tgatccagtg gccggcggag
     2761 ccctgcttgt cgtagtccat caataccggc cgctcacccc gcagcgcaaa gtgactggcg
     2821 agattgatcg ccaccgtcgt cttgcctgat ccgcccttcg ggttcagcac cagaatgcgc
     2881 tgcatgtagt tctcgtagag ccgtgaaaat tatgtcctgt ttgcagacta cgcagcgctc
     2941 agggggcccg caagcgccgc cgtcagaact gagcactcgg tcttacttaa tcgcgaattt
     3001 gcctgaccaa tctgcgagca cgcgcacggg gtgcctgtga gtcagaacac tccatcggcc
     3061 acgtccgatc tttaggcttc cccacggtca cacaaaggtg cgcaatggaa agattcctgc
     3121 tgctctggga cgacatggac gactggctgt ccgccgccct gcacgtgttt ggccgccgga
     3181 tggcttgaaa ccttaacgct tagcccctat accgctgctt ccattcggta acactagcgg
     3241 cgggtaccat gtcgagtgag cgcgagactc tgggcttcca ggctgaagtc cgggagttgc
     3301 tacatctgat gattcattcc ctgtacagcc acagggaaat cttcctgcgg gaactgatct
     3361 caaacgcgtc ggacgcgaat gacaaactgc gcttcctggc gctgtcggac ccgcagatgc
     3421 tgggcaagga gagcgaactc ggtatctgga tcgataccga cgccaaggcc ggcacaatta
     3481 cggtgcgcga caacggcgtc ggtatgagtc gcgaggatgc cgtcgtccac ctcggcacga
     3541 tcgccaagtc cggtacgcgc gaattcttca agcgcctgac gggcgatcag cagaaggacg
     3601 ctcagctcat cggtcagttc ggcgtcgggt tctattccgc gttcatcgtg gccgacaagg
     3661 tcgaagtact gacccgccgc gccgataccc ccgcggaagc aggggtgcgc tgggaatcca
     3721 gcgccgatgg ccagttcacc gtggagagcg tgaaccgcac cgagcgcggc accagcgtga
     3781 ccctgcacct caaggaggac gccaaggaat tcagcgaccc ctggcgcctg cgatcgctgg
     3841 tacgccgtta ctcggatcac atcgcgttcc ccgtccggat gagcaaggag ggcgcggcca
     3901 cgctggaata cgaggaggtc aaccaggcgc aggcgctgtg gacgcgagcg cgtagcgaga
     3961 tcaaggacga ggagtaccgt gatctctatc gccacatatc gcacgacggt gcggatcctc
     4021 tggcgtggag ccacaaccgc gtcgaaggca agcgcgagta tacgagcctg ctctatatac
     4081 cttcgcgcgc gccgttcgac ctgtggcagc gtgaagctgc ccgcggcctc aagctgtacg
     4141 tacggcgcgt gttcatcatg gacgatgcag agcagtttct gccgctgtat ctgcgcttcg
     4201 ttcgcggaat cgtcgattcc aacgacctgc cgctgaatat ctcgcgcgag ctgctgcagc
     4261 aggatcgcga agtcgaggcc atgcgttcgg cgatcacccg gcgcgcgctc gagctattgg
     4321 cacgtctcgc caaggaagag accgacaagt atgcccagat ctggcgcgag ttcggccggg
     4381 tgctgaagga aggcctgagc gaggatccga ctaaccgcga ccgattgctg ccgctgttgc
     4441 gctatgcaag cactcaccag gaagccaatg aggagaatgt cagcctgagc gactacgtcg
     4501 cgcgcatgcg cgaaggccag gagcgcattt actacatcgt ggccgagaca gtggatgcgg
     4561 cgcgggcgag cccgcatctg gagctgctca agcagaaggg cattgaagtg ctgctgatgg
     4621 gcgaccgtgt cgatgattgg gtgatgggtc aaatcagcga attcgaaggc aagaaattca
     4681 aagatgtctc gcgcggtgat ctcgaactgg gcggcctggc cgaccaggcc gaacgcgccc
     4741 gaatcgatga ggagcgcaag gagcacaagg cattgctcaa gcgcttcaag gacgccctgg
     4801 gcgatcgcgt actggaagtg cgggtcagcg agcggttgcg cgactctccg gcatgtcttg
     4861 tgcatggcga gggcgagatc tcggcgcaga tgcggcgtat cctctcggcc agcgggcagt
     4921 cgctacccgc cggcaagccg gcgctcgaag tgaatgtcgc gcatccgctc gtgcgccaca
     4981 tggacgggat cgcgcaggag ccggccttcc aggaattggc gctgctactc tacgatcaag
     5041 cgacgctcac ggacgagggc caggttgcga acccggctga tttcagccgc cgactgaaca
     5101 atctgctggt gcgcctggtg gcgggctcgg cgcagccggc ggcgccttga cggatcgctg
     5161 ttcgattgac ctgagagggg aacaagcaat gggcactgaa acaccgaccg acgcagaact
     5221 caaagcacgt ttgactccgc tgcagtatca ggtgacacag aagaagggca ccgagccggc
     5281 ctttaccggc aagtacgctt acaacaagga gaagggtcta tacaactgcg tgtgctgcgg
     5341 acaggaattg ttcagctcgg acaccaaata cgactccggc tccggctggc ccagtttctg
     5401 gctgccgctg gcgagcgacc gcgtgcgcac ccgcatcgac acgagccatg gtatggagcg
     5461 caccgaagtc acctgctcgc gatgtggcgc ccacctcggg cacgtcttcg atgacggtcc
     5521 gcagccatcg gggctgcgtt actgcatcaa ctcggcatca ctcggattcg attccgtcaa
     5581 gaaaccttga gtttcagacc gccgaaggcg caagcgctca gcatccgggg tccggccggg
     5641 gcgctcgagt cgctgctgga agatccgcag gcgcccgtag ccgccggatt tgcagtgatc
     5701 tgtcatccgc atcccttgtt tggcgggaca atgcagaaca aggtggtgca caccctcgga
     5761 cgtgcctgcc aggagttcgg gctcgcaacg ctgcgattca actttcgcgg ggtcggcgcg
     5821 agcgatggca gccacgatga gggtcgggca gaaatcgagg atgccatggc ggtgatcgcg
     5881 tggggacgcc agcgctggcc cgatggacca ctgacacttg ccggcttttc tttcggcgcc
     5941 atggtggcgc tggcggccgc ggtgcgctcg gcaccctcaa agctgatcac ggtagcgcca
     6001 gcggttggcc gacctgagtt ttccagcgcg gccaaaccca actgcccctg gctgattgtg
     6061 cagggggacg ccgatgagtt ggtggatatc cgcgacgtgc gtgcatttgc ggcgcgcttc
     6121 gagccagcgc cgaaactggt ggtgatgtcc ggcgtcgaac acttctttca cggccgcctg
     6181 acgcaactgc gcgacgaagt gctggcgttc ctgaaaaacg aagaagcccg gtaaagcttc
     6241 actctaccgg gcttccctta agatgcttgt gcggtgcgag cgatccttca gtcgctcaat
     6301 tcaccatcga cttgagattc ttcaacggga gcgcgcgcac cttcttgctc gcgggctttg
     6361 ccttgaaggt catcttttcg ccggtgaatg gattgatgcc ttcacgcgcc ttggtcgccg
     6421 gccgcttcac gaccttcagc ttgagcagac cgggaagcgt aaacaagcca ttgccgcgca
     6481 ggctcttctt tatgatgcca ttcagcgact cgaaaacctc gctgacctgc ttacgggaaa
     6541 gttccgagtc cttggaaatc tgggccagaa tttccgactt cgtcggtgcc ccaccttttt
     6601 ttgccattgc cgaatctcct ggtgtatgcg acatctgtct cgatgatcgc gtagcactga
     6661 aaagctacac gaaacggcgc gcaacatagc ataccgctga gctgacgcaa atgctttttc
     6721 ggtcacacgc actgcgaaaa tgcctttttt tataggcccg cgtgcatcga cgtgcgtcgc
     6781 caggcgcgtt agtggacgag aatcagcaac atttggatga gaatgccggc ccacatcggc
     6841 gagaagtcga cgccgcccag tggcggaata acgcgccgaa acggccgcag caccggctcg
     6901 cacagcgcca ccagcaggcc ctgggccggc gaataggtac ccggggcgac aaacgatagc
     6961 agcgcataga cgacaatggc gaagaaatag gtccacaggg tcgtgcgaac gatctcaatg
     7021 gccgcgaacc gcagccacaa cagcaccggc agcgggccga agcccttcaa cagtggtgcg
     7081 gccgcgactt ccagcaacgc aaatacgatc accgctacgc atgaagcggt atccagcctc
     7141 ccgatcgcgg gcagcagacg tcgcagcggc acgatgatcg ggttggtgat gtggaccagg
     7201 gatctggcca gggggttgcg gaaatccgca cgcgaccatt gcagcagcag ccgcagcagc
     7261 accgcgagca ggcaaaggga caatagggta tcgaccaggt agatcagcgc atccatctat
     7321 ttgcgctcgg gtgaggtggt tgcgctcaga ctattggcga gttcggcgct gcgcgcaacg
     7381 ccggcctgta gagccgatgc aaccagctgc gtgaagccgc cctgctccag gacgcgcacg
     7441 gccgcttccg tggtaccgcc tttcgaggtg acggctgcac gttcttcgac caggccgaca
     7501 ccttcgtttg ccagcatgcc agcaccatgc agggtcgcgc gcgccagctg caaggcggtg
     7561 tcgcgcgtca gtcccaggcc ctcggcagcg tgcgccagct tctcagccaa tagaaagaag
     7621 tacgccggtc ctgcgcccga gagggccgtc acgacgtcca gctcatcttc actgcgcagc
     7681 cagacggctt gtccggacgc ctgtccgatc agttccgcgt ccctgtgctg ctcggcgctg
     7741 atgcctgggg ctgcgaacag gccggtgacg ccggcaccga gcagtgccgg gcggttaggc
     7801 atcgcgcgca cgatggcgat gcccgggccg caggcggtgc gcaagtccgc aatacgcagg
     7861 cccgcggcaa tcgagaacaa cagggggcgc gaggcctgca aggccgggcg cagcgcctgg
     7921 aggatgtgac cggcctgctg cggtttgacc gccaacacca ccagctgcgc accgtcgagc
     7981 atttcctggt tgtcggcgct gacacgtatg ccgaagtcgc gttgcagtcc cgctcgcgcc
     8041 tgcgcatcgg gctcgccgac gctgatgtgt tcagccggca ccgcgtgccg cttcaaaccc
     8101 ccgatcagcg cccgggccat gttgcccccg ccgatgaagg cgatacgaac cgctaccgat
     8161 tgctgcatga caccgcattc tactcgtcgg acgatgcggt tcgcgggccg aaaatcgccg
     8221 tgccaacgcg gatcagggtc gcaccctcca ggatggcgtc gcgaaaatcg gcgctcattc
     8281 ccatggagag cgtatccagg ccagcgcctt gcgcgttcag ttgttcctgc agccgacgca
     8341 gcgcgccgaa gtgtcgccgg ttgtctgcgc tgctgagctg cgctggcagg atgcacatca
     8401 ggccccgcag ctggagccgg ggcagaagcg ccacggctcg ggcaagttcg cttacccgct
     8461 ccggtgcgac gcccgccttg ccggactcac cggcgatgtt gacctgcagg cacacctgta
     8521 gtgccggcgc gtaatgcggg cgctgtgcgg acaggcgctg cgcgatcttc agccggtcga
     8581 taccgtggac ccagtcaaag ctttcggcga ctgagcggct cttattggct tgcaagcgcc
     8641 cgatgaagtg ccagctggct tgcagatcac gcagcgattc cagtttccgg gtagcctcct
     8701 gcaggtagct ctcgccgaag tgcagcactc cactgaccgc ggcagctcgc acgctggacg
     8761 cttcgtggcc cttgctgacc gcaatgactg tgatcgattc cacagtccgg cgcgctgcgc
     8821 ctgccgcttc ggcgacctgg gcgcgcagct gctgtagatt ccccgaaatc aatgtgtctg
     8881 ctgctgaatt gtgcggagcg attaacatat gggttgtcgg ccccctccgc tatactcagg
     8941 ccgcttgccg ctggcaccag cggaataggg taggatcgaa tggctgtcga tatcgcgcaa
     9001 ttgcttgcgt ttgcagtaaa gaataacgct tcggatctgc atctgtcggc cggtgtaccc
     9061 cccatgatcc gggtagacgg ggacatgaag cgcatcaaca tgcccgcact gacccacaag
     9121 gaagtgcacg gcatggtgta tgacatcatg aacgacaagc aacgcaaagc gtacgaagaa
     9181 ttcttcgaga cggacttttc gttcgagatc cccaagctgg cgcgtttccg cgtcaatgca
     9241 ttcaatcaga atcgcggagc cggtgcagtc ttccgtaaca ttccgtccgc gattctcag
//
LOCUS       DATMRM010000402         8255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1814940, whole genome shotgun sequence.
ACCESSION   DATMRM010000402 DATMRM010000000
VERSION     DATMRM010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8255
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814940"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..639
                     /gene="sucD"
                     /locus_tag="VNZ06_12165"
                     /pseudo
     CDS             1..639
                     /gene="sucD"
                     /locus_tag="VNZ06_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010327286.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
     gene            906..2537
                     /locus_tag="VNZ06_12170"
     CDS             906..2537
                     /locus_tag="VNZ06_12170"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HWW21556.1"
                     /translation="MADNDTLKVALAQINLLVGDVRGNLERAITAARRARDELRADLV
                     LLPELTLSGYPPEDLLFHRGLRLQIERALIELQKAVPEIGVMVGFPEYVEAQIYNAAA
                     LLHGGAELARHRKACLPNYRVFDEKRYFTPGTQPTVVSFKGMRLGLLICEDVWEAGPV
                     AAAAKQGAQALMIINASPYELHKQREREQVLSDRIREVHLPAAYVNLLGGQDELVFDG
                     NSFAMDAQAQVAMRADAFKDGLYCVEFARRDGALVPLAASIAPELADEESVYRALVLG
                     VRDYVTKHGFPGVVLGLSGGVDSALTLAIAVDALGANSVQAVMMPSRYTSQMSREDAT
                     AQARTLGVCLSSISIEDMYQVTLQALQGEFAGRQPDATEENIQARCRGVLLMGISNKT
                     GRMLLTTGNKSEMSVGYATLYGDMAGGFAPIKDCSKMLVYRLARYRNSLSPAIPRRVI
                     EREPTAELRHDQRDTDSLPPYDVLDPILEAFIEDDLSVDEIVERGFDRAVVARVLDMV
                     KRAEYKRRQAPPGVRVSRRAFGRDWRYPITNGYRR"
     gene            complement(2563..3402)
                     /locus_tag="VNZ06_12175"
     CDS             complement(2563..3402)
                     /locus_tag="VNZ06_12175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HWW21557.1"
                     /translation="MSSKFSAASGRWRLLGALFLVSLALLGGCQSRATRNAKKLNAEQ
                     LYAAAHKSMLANDYEASIKQYEALSSRFPFTNVAKQARIDLIYVYYRKGEKETAIDAA
                     DQFLRENPTHPRVDYAWYMKGMIYFERTPYSIERFFGVDMAKRPPSDALKSINAFSTV
                     VTQYPNSDYAHDALKRMTYLRNRLAEYEVNVARWYIKRGAYIAAARRAEGVVEQYDGA
                     PAEQPAMAILLQCYERLGYTELASNVKRVYDYNYPPQSESVNARGKRWWQFWASARPT
                     AIN"
     gene            3458..4438
                     /gene="rluD"
                     /locus_tag="VNZ06_12180"
     CDS             3458..4438
                     /gene="rluD"
                     /locus_tag="VNZ06_12180"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: protein motif:HMM:NF008385.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HWW21558.1"
                     /translation="MSPQFQTHQFDIPAAAAGQRLDQALAAALPQYSRARLQRWIREG
                     AVRVAGRAARARDRVHGGEQIELNATFSADSSVEPEAMSLAIVHQDHALLVVNKPAGL
                     VVHPGAGNPSHTLQNALLAHDPTLARVPRAGLIHRLDKDTSGLLIVARTPEAHTRLVT
                     QLAAREIHREYLALVLGAPTGGARIDQPIGRHRSQRTRMSVRGDGRQALTHYRIEERF
                     LAHTLLRVQLETGRTHQIRVHLAHAGYPVVGDPVYGGRRRLVAGERGELNELLRAFQR
                     QALHAQRLRFAHPLEAHALTLEAPAPQDFQSLLKALRADARAAESAAARR"
     gene            4435..5202
                     /gene="pgeF"
                     /locus_tag="VNZ06_12185"
     CDS             4435..5202
                     /gene="pgeF"
                     /locus_tag="VNZ06_12185"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HWW21559.1"
                     /translation="MSTLSLLPVHWPAPRPVRAACTLRDGGVSADPYASFNLAAHVGD
                     DPRAVASNRARLLTGLRLPHEPLWLNQVHGTQVLEASGALSPGAGPPPQADAATTGTP
                     GIVLAVLVADCMPILLCRRDGAGVAVAHAGWRGLAAGVVEAAMAKLGVGAEQVLAWLG
                     PSIGPSHFEVGDEVRAAFCAHDARAESGFTRNQRGRWQCDLYQIATQRLRSLGVGSIH
                     GDRLCTYSERERFFSHRRDGRTGRMAALIWLEPNRFA"
     gene            5199..5981
                     /locus_tag="VNZ06_12190"
     CDS             5199..5981
                     /locus_tag="VNZ06_12190"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HWW21560.1"
                     /translation="MTLLWIVGATFLGGALSAALASAFLLLGDQQRGWLLPHLISFAT
                     GAMLAAALVGLLPEAITSAGPLHVQSIGVAVLAGIGLFFVLEKLVLWRHCHVEQCDTH
                     SPHEVGRDQAAGWIVLFGTGMHNAFDGVLIAAAFLTDTRLGMMTTIAITAHEVPQELG
                     DLAVLLHARMSARRALWFNAASSLTSILGALAGYFALRGALGILPYAISVAAASLLYV
                     AVADLIPGLHRRVDPRGASAQVLLIAAGASLIWWAQRWLPDA"
     gene            6086..>8255
                     /gene="clpB"
                     /locus_tag="VNZ06_12195"
     CDS             6086..>8255
                     /gene="clpB"
                     /locus_tag="VNZ06_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HWW21561.1"
                     /translation="MRMDKLTSRFQAALADAQSLAVGRDHQFIEPVHLLAAMLDAEGG
                     GVRPLFVKAGVDVNRLRTLLGNALDRLPRVEGTSGDMQVSTELNRLLNVTDKLAQQRG
                     DQYIASELFVLAAFEDRGVIAKALKEVRASREAIEKAIAEVRGGEGVNDAGAEEKRGA
                     LEKYSIDLTARARAGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LALRIVNGEVPEGLKSKSILALDMGSLIAGAKFRGEFEERLKAVLSELAKQEGKVILF
                     IDELHTMVGAGRAEGAMDAGNMLKPALARGELHCVGATTLDEYRKYIEKDAALERRFQ
                     TVLVDEPSVEDTIAILRGLKERYEVHHGVEITDPAIVAAATLSHRYITNRQLPDKAID
                     LIDEAAARIRMEIDSKPESLDRLERRIIQLKIEREALNKEEDEASKRRLATLEHTLST
                     LEKEFADLQEVWKAEKASLQGATQIKEDLEHAKLEFEQARRASDLARMAELQYGRIPE
                     LEKRLAGAQGAEQTTPQLVRNSVTDEEIAEVVSKWTGIPVSKMLEGEKDKLLRMEQAV
                     EKRVVGQDEAVRIVSDAIRRSRAGLSDPRRPNGSFLFLGPTGVGKTELCKALAEFLFD
                     TEEALVRIDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAIRRRPYAVVLLDEVEKA
                     HQDVFNVLLQVLDDGRLTDGQGRTVDFNNTVIIMTSNLGSQ"
BASE COUNT         1458 a         2494 c         2830 g         1473 t
ORIGIN      
        1 ccctacgccg ccgatgcgat cctggaggcg gtcgatgccg gtatcgagct ggtggtgtgc
       61 atcaccgagg gcttgccggt gaatgacatg gtgaaggtca aggcggcgct cgccggttcg
      121 cccacgcggc tgatcggacc gaactgcccc ggtatcatca cgccggagga atgcaagatc
      181 ggcatcatgc cgggcttcat tcacaagcgt ggttgcgtgg gcattgtttc acgctctggc
      241 accctcactt atgagaccgt gtttcagacc acgaacaatg gactcggaca gagtacctgc
      301 atcggtatcg gcggcgatcc ggtgcgcggc atgaatttca ttgacgtcat tgaactgttc
      361 gaacgcgatc cgcagaccga aggcatcatc atggtgggtg agatcggagg ctcggacgaa
      421 gaggctgccg ccgaacacat acgacgctac gtgagcaagc cggtcgtcgc ttacatcgcc
      481 ggcgtcaccg ctcccgcggg caagcgcatg gggcatgccg gcgccgtgat cgcaggcggt
      541 aaaggtaccg ctgccgacaa gtatcgcgcg ctggatgccg ccggcgtgcg caccgtgaaa
      601 tcgccagccg aactcggcag tgccatggcg gagctgctgg gcaagcgtcg agccggggcc
      661 gcgagacagg ccgcgcgcga tgcagctcag gccgccaagg atgccaaagc gctgcgcgag
      721 cgatccgcga agcacccgcg cagcgcgact ggccggcgtc aacatcagcc gactacccag
      781 gccacgaaac gcgtgccgaa gcgcccgccg cggagcgtgc gcgccaaggc cgcgaagcga
      841 gccaagcgtg ctggtagagc acggcgctag aacgcacgcg cccatgctga ttcaccggag
      901 agccggtggc cgataacgat acgctcaagg tggcgctggc gcagatcaac ctgctggtcg
      961 gcgacgtgcg tggcaatctg gaacgcgcga tcacggcggc ccgtcgtgcg cgcgacgagt
     1021 tgcgtgctga tctggtgctg cttccggagc tgacgctgtc gggctatccg cctgaagatc
     1081 tgctgtttca tcgcggactg cgcctgcaga tcgagcgcgc gctgattgag ctgcagaaag
     1141 ccgtgcccga gatcggcgtg atggtcggct ttccggagta cgtcgaagca cagatctaca
     1201 atgccgcggc attgcttcat ggcggcgcgg agctcgcgcg gcatcgcaaa gcctgtctgc
     1261 cgaactaccg tgtgttcgat gaaaagcgct atttcacgcc ggggacgcag cccacggtcg
     1321 tgtcgttcaa gggcatgcgg ctcggactgc tgatctgtga ggacgtctgg gaggcggggc
     1381 cggtcgcggc ggcggcgaag cagggcgcgc aagcgctgat gatcatcaac gcttcgccct
     1441 acgagttgca caagcagcgc gagcgcgagc aggtgctcag cgatcgaatc cgcgaagtgc
     1501 acctgccagc agcctacgtg aatctgctgg gcggtcagga tgagctggta ttcgacggca
     1561 attctttcgc gatggatgca caggcgcaag tggcgatgcg tgccgatgcg ttcaaggacg
     1621 gtctgtactg cgtcgagttt gcgcgtcgcg acggcgcgct ggtaccgctg gcggcgagta
     1681 tcgcccctga gcttgcggat gaggagagtg tttaccgggc cctggtgctc ggtgtgcgcg
     1741 attacgtgac gaagcacggc tttcccggtg tcgtgctcgg cctgtcgggc ggggttgatt
     1801 cagcgctgac gctggcgatc gcagtggatg cgctgggcgc caacagcgtg caggcggtga
     1861 tgatgccctc gcgctatacc tcgcagatga gtcgcgagga tgcgaccgcg caggcgcgta
     1921 cgctgggagt ctgcctgagc tccatttcga tcgaggacat gtatcaggtg acactgcaag
     1981 ccctgcaggg cgagttcgca ggccgtcaac ccgacgccac ggaggagaac atccaggcgc
     2041 gctgtcgcgg cgtgctgctg atgggcatct ccaacaagac cggccgcatg ctgttgacaa
     2101 ccggcaacaa gagcgagatg tccgtgggct atgccacact ctacggcgac atggcgggcg
     2161 gtttcgcccc catcaaggat tgcagcaaga tgctggtcta tcgattggca cgctaccgca
     2221 actcgctgtc gccggcaatt ccgcgcagag tgatcgagcg cgaaccgacg gcggagttgc
     2281 gccacgatca gcgcgacacg gattcgctgc cgccctatga cgtgctcgat ccgatccttg
     2341 aggccttcat cgaggatgat ttgtcggtgg atgaaatcgt cgagcgcgga ttcgaccgtg
     2401 ccgtggtcgc tcgcgtgctg gatatggtga agcgcgccga atacaagcgc cgccaggctc
     2461 cgcctggcgt gcgcgtcagc cgtcgggcct tcggacgcga ctggcgctac ccgattacga
     2521 acggctatcg tcggtaagcg gccgggtcgc tagcgaggat ggctagttga tcgccgtggg
     2581 cctggcgctc gcccagaact gccaccagcg tttgccccgc gcgttcaccg actcactctg
     2641 cggcggatag ttgtagtcat agacgcgctt cacgttgctg gccagttcgg tatagcccag
     2701 tcgctcgtag cactggagca ggatcgccat cgcgggctgt tccgccggtg cgccatcgta
     2761 ttgctcgacc acgccctcgg cgcgtcgcgc ggcggcgatg taagcgccgc gtttgatgta
     2821 ccagcgggcg acgttgactt cgtattcggc caggcggttg cgcagatagg tcattcgctt
     2881 gagtgcgtcg tgcgcgtagt cgctgttggg gtattgggtg acgacggtac tgaaggcgtt
     2941 gatcgatttg agcgcatcgc tcggcgggcg cttggccatg tccacaccga agaagcgctc
     3001 gatggagtag ggtgtacgtt cgaagtagat catccccttc atgtaccagg cgtagtccac
     3061 gcgcggatgg gtcgggtttt cgcgcaggaa ctggtcggcc gcatcgatcg cggtttcttt
     3121 ttcgcccttg cggtagtaga cgtagatgag atcgatgcgc gcctgtttgg cgacgttggt
     3181 gaacggaaat cgggagctga gcgcctcgta ctgcttgatc gacgcctcgt agtcgttggc
     3241 aagcatcgat ttgtgagccg cggcatagag ctgctcggca ttgagtttct ttgcgttcct
     3301 ggtggcgcgc gactggcagc cgcccagcag ggccagcgat accaggaata aggcgcccag
     3361 cagccgccag cggccggagg ccgcgctaaa ttttgagctc aatgatggac ctaagggaaa
     3421 atcaattgat aatcgaccca gtatagctga ccgcccgatg tcaccgcagt tccagaccca
     3481 ccagttcgat attcccgctg cggcggctgg ccagcggctg gatcaggccc tggcggcagc
     3541 cttgccacag tattcacggg cacgcctgca gcgctggata cgtgaaggcg cggtgcgggt
     3601 ggccggccgg gcggcgcgcg cccgagatcg cgtgcacggg ggtgagcaga tcgagctgaa
     3661 cgcaaccttc agcgccgaca gctcggttga gcccgaggcg atgtcgctcg cgatcgtgca
     3721 tcaggaccac gcgctactgg tcgtgaacaa gcccgccgga ttggtggtcc atcctggcgc
     3781 cggcaatcct tctcacacgt tgcagaatgc cctgctggcg cacgatccaa cgctggcgcg
     3841 ggtgccacgc gcaggactca tacaccggct cgacaaggac accagcggtc tgctgatcgt
     3901 tgcccggaca cccgaagcgc acacgcggct ggtcacgcaa ctggccgcgc gcgagatcca
     3961 tcgcgagtat ctggcgctgg tgctgggcgc gcccacgggc ggggctcgca tcgatcagcc
     4021 gatcggtcgt catcgcagcc agcgcacgcg catgagcgtg cgcggcgatg gtcggcaggc
     4081 gctcacgcac tatcggatcg aggagcgctt tctcgcccat acgctgttgc gcgtgcaact
     4141 ggagaccggc cgcacccatc agattcgcgt gcatctcgca cacgccggct acccggtggt
     4201 cggtgacccg gtctatggcg gacggcgcag actggtcgcc ggcgagcggg gcgagctgaa
     4261 tgagctgctg cgggcgttcc aacgccaggc gctgcacgcc cagcggctgc gcttcgcgca
     4321 tccgctcgag gctcatgcgc tgacgctcga agctccagcg ccgcaggatt tccagtcgtt
     4381 gctcaaggcc ctgcgcgccg atgcacgcgc ggccgaatcc gcagccgcgc gccgatgagc
     4441 accctgtcgc tgctgcccgt gcactggccc gcacccaggc cggtgcgcgc ggcctgcacg
     4501 ctgcgcgatg gcggggtcag cgccgatccc tatgcatcct tcaatctcgc ggcacacgtc
     4561 ggtgacgatc cgcgggccgt cgcctccaac cgcgcgcgct tgctgactgg gctgcgcttg
     4621 ccgcacgagc cgctctggct caatcaggtg cacggtacgc aggtgctcga ggcgagtggt
     4681 gccctcagcc ctggcgcagg cccaccgccg caagcggatg ccgcgacgac tggcacgccg
     4741 ggaatcgtgc tcgccgtgct ggtcgccgat tgcatgccga tattgctgtg tcgtcgcgac
     4801 ggagccgggg tcgcggtcgc gcacgccggc tggcgcggcc tggcagccgg agtcgtcgaa
     4861 gcggcgatgg ccaagctggg cgtgggggcc gaacaggttc tggcctggct ggggccctcg
     4921 atcggcccgt cgcatttcga agtgggcgat gaagtgcgcg cggctttttg tgcgcacgat
     4981 gcgcgtgccg agtcgggatt cacccgcaac cagcgaggac gctggcaatg cgatctgtac
     5041 cagatcgcga cgcagcgcct gcgctcgctc ggcgtcggtt cgatccatgg cgacaggctc
     5101 tgtacctaca gtgagcgcga gcgtttcttt tcccatcgcc gagacggcag gaccgggcgt
     5161 atggcggcgt tgatctggct ggagcctaat cggttcgcat gacgctgcta tggattgtcg
     5221 gtgcgacgtt cttgggtggt gcactcagtg ccgcgctggc gagcgccttc ctgctgttgg
     5281 gcgaccagca gcgtgggtgg ctgctgccgc atctgatcag tttcgccacc ggcgccatgc
     5341 tggcagccgc gctggtgggc ttactcccgg aagcgatcac aagcgcagga ccgctgcacg
     5401 tgcagtcgat cggcgtggcg gtgctggccg ggatcgggtt gttctttgtc ctggaaaagc
     5461 tggtgctgtg gcgccactgc catgtcgagc agtgcgatac acactcgccc catgaagtcg
     5521 gtcgcgacca ggcggccggc tggatcgtgc tgttcggcac cggcatgcac aatgcgttcg
     5581 acggtgtgtt gatcgccgcg gcgtttctga ccgatacccg gcttggaatg atgacgacga
     5641 tcgcgatcac ggcgcacgaa gtcccgcagg agctgggcga tctggctgtg ctgctgcacg
     5701 cccggatgag tgcccggcga gcactgtggt tcaacgcggc cagcagtctg acctcgatcc
     5761 ttggagctct ggcaggctac ttcgcgctgc gtggtgcgct ggggattctg ccttatgcga
     5821 tttcggtggc tgccgcctcg ctgctctacg tggcggttgc cgatctgatc ccgggcttgc
     5881 accgcagggt cgatccgcgc ggcgcgagcg cccaggtgct gctgatcgca gcaggagcaa
     5941 gcctcatctg gtgggcgcag cgctggctgc cagacgcttg aattcactgg cttcctcccc
     6001 cactgaaggc agtacgttaa tcacggattg cgcgcccgag ggttagtcgc gcaagctgca
     6061 attttagggt acaggtcaaa gacttatgcg gatggacaaa ctcaccagcc ggttccaggc
     6121 ggcgcttgcg gatgcgcagt cgctggccgt gggccgcgac caccagttca tcgaaccggt
     6181 acatctgttg gcggcgatgc tcgatgccga gggcggcggc gtacggccgc tgttcgtcaa
     6241 agccggcgtc gacgtcaatc gcctgcgtac cctgctcggc aacgcgctcg atcgcctgcc
     6301 cagggtggaa ggcacctcgg gcgacatgca ggtctccact gagctgaacc gactgctgaa
     6361 tgtcacggac aaactggcgc aacagcgcgg cgatcagtac atcgccagcg agctgttcgt
     6421 gctggcggcg ttcgaggatc ggggcgtgat cgcgaaggcg ctcaaggagg tgcgcgcctc
     6481 ccgggaagcg atcgagaagg ccatcgccga agtgcgtggt ggcgagggtg tgaatgatgc
     6541 gggtgccgag gagaagcgtg gcgcgctcga gaaatactcc atcgatctga ccgctcgcgc
     6601 acgtgccggc aagctcgatc cggtgatcgg acgcgacgat gagattcgtc ggaccattca
     6661 ggtactgcag cgtcgcacca agaacaaccc ggtgctgatc ggcgagccgg gcgtcggcaa
     6721 gacggcgatc gtggagggcc tggcactgcg cattgtcaat ggcgaggtgc cggaaggact
     6781 caagagcaag agcatcctgg cactcgacat gggctcgctg atcgcaggcg ccaaattccg
     6841 cggcgagttc gaagagcgcc tcaaggcggt cttgagcgaa ctggcgaaac aggaaggcaa
     6901 agtcatcctg ttcatcgatg aactgcacac catggtcggc gccggtcgcg ccgaaggcgc
     6961 gatggatgcc ggcaacatgc tcaagccggc cctggcgcgc ggtgagctgc actgcgtggg
     7021 tgcgaccacg ctcgatgaat atcgcaaata catcgagaag gacgccgcac tggagcgtcg
     7081 cttccagacg gtactggtcg atgagcccag cgtcgaagat acgatcgcca tcctgcgcgg
     7141 cctgaaggag cgctatgagg tgcatcacgg ggtcgagatc accgatccgg cgatcgtggc
     7201 cgccgccacg ctatcgcatc gctacatcac caaccgccag ctgcctgaca aggcgatcga
     7261 tctgatcgat gaggctgcgg ctcgcatccg catggaaatc gattccaagc ccgagtcgct
     7321 ggatcggctg gagcgcagaa ttattcagct caagatcgaa cgcgaggcgc tcaacaaaga
     7381 ggaggacgag gcctccaagc ggcggctggc gacgctggag cacaccctga gtacgctcga
     7441 gaaggagttt gccgatctgc aggaagtgtg gaaggccgag aaggcctcac tgcagggtgc
     7501 cacgcagatc aaggaagacc tggaacacgc gaagctcgag tttgagcagg cacgtcgggc
     7561 gagcgatctg gcccgcatgg ccgaactgca gtacgggcgc attccggaac tggagaagcg
     7621 gttggctggc gcgcagggcg cagaacagac cacaccgcaa ctggtgcgca acagcgtcac
     7681 cgatgaggaa atcgcggagg tggtttccaa atggaccggc attccggtgt cgaagatgct
     7741 ggaaggcgag aaggacaagc tgttgcggat ggaacaggcg gtcgagaagc gcgtggtggg
     7801 tcaggacgag gccgtacgca tcgtttccga cgccatccgt cgctcgcgcg cgggactgtc
     7861 cgatccgcgt cggcccaatg gctcgttcct gttcctgggt cccaccggcg tgggtaagac
     7921 cgaactgtgc aaagcgctgg ccgagttcct gttcgacact gaagaggcgt tggtcaggat
     7981 cgatatgtcc gagttcatgg aaaagcactc ggtcgcgcgc ttgatcgggg cgcctccggg
     8041 ctatgtcggc tatgaggaag gcggctacct gaccgaggcg atccggcgcc gtccctatgc
     8101 ggtggtgctg ctcgacgaag tcgagaaggc gcaccaggac gtgttcaatg tgctgttgca
     8161 ggtgctcgat gatggtcgtc tgacagacgg ccagggtcgc accgtggact ttaacaacac
     8221 cgtgatcatc atgacctcga atctggggtc gcagg
//
LOCUS       DATMRM010000403         6972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4269549, whole genome shotgun sequence.
ACCESSION   DATMRM010000403 DATMRM010000000
VERSION     DATMRM010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4269549"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VNZ06_12200"
     CDS             complement(<1..508)
                     /locus_tag="VNZ06_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21562.1"
                     /translation="MKRKPSVLMMFALTASFVSACGGGDNGQHGNSGSSGLGRGALLQ
                     TPPTLTMSITAQTLAARLTADATAGGAQTVLTSVLTLAGFPQCDINVYHVEYVTVGGQ
                     NETTTASGALMVPSGGGASCSGPRPIVLYAHGTQASKSFNIADLSNSENAEGILLAAF
                     FAARGYIVV"
     gene            890..1444
                     /locus_tag="VNZ06_12205"
     CDS             890..1444
                     /locus_tag="VNZ06_12205"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWW21563.1"
                     /translation="MKYLPAVTLLLGLTSVSGAEVYNLDTTATRVGFDIEHFGLHWVS
                     AHFRSFQGDFVFDRTGSASRVDVIVQTDSVDCGDSHWNPHLRSPEWLDVQQFPLMTYR
                     SRHIEFEGGDRAEASGDLTLHGVTHPVVLDVSQLRCNGDGTDQACRFVAHARVKRSDY
                     GLPHGFWTGGDQVDITIVGVGMHH"
     gene            1511..3160
                     /locus_tag="VNZ06_12210"
     CDS             1511..3160
                     /locus_tag="VNZ06_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005246259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HWW21564.1"
                     /translation="MRGLMMDMPMLISRLICHADREHGDTEIVSRTVEGPIHRYTFHD
                     AHHRSRQLANALLTQGVRFGDRIATLAWNTYRHFELFYAISGIGAICHTVNPRLFAEQ
                     LLYIFNDAEDRYIFLDLTFVEIVQRIAPQLPHVRGFVILTDRAHMPQTSLPNALCYEE
                     LVGAASDQFEWPQFDEHTASSLCYTSGTTGNPKGVLYSHRSNILHAYAMALPDTYCLS
                     ATDIMLPAVPLFHANAWGIPYACALVGTKMVLPGSQLDGASLHELFEREGATVTAGVP
                     TVWFGLLGYMKQHGLRFSTLNRALIGGSACPAAMIQTFEEEYGVRVLHAWGMTELSPL
                     GTLTAFKAKHTQQSAAERRALQSKQGRVIFGVEMKIVDDAGNELPRDGRTFGNLMVRG
                     PWICQHYFKAEHVQILQDGWFGTGDVATIDPDGYMQITDRAKDVIKSGGEWISSIELE
                     NCAVGHPDVAEAAVVGVPHAKWGERPLLLLVRKPGASPSREDVLQYLAPRIAKWWLPD
                     DVVFVDALPHTATGKLLKTKLREDFRDHLLKQTDRPATQPR"
     gene            complement(3148..3348)
                     /locus_tag="VNZ06_12215"
     CDS             complement(3148..3348)
                     /locus_tag="VNZ06_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21565.1"
                     /translation="MKLLTLLVWCLLFVVCWPVALFALILWPIVFLLTLPFRLVGLTF
                     DALFAFLRAILMLPARVLGHRG"
     gene            complement(3351..4682)
                     /locus_tag="VNZ06_12220"
     CDS             complement(3351..4682)
                     /locus_tag="VNZ06_12220"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HWW21566.1"
                     /translation="MATIQSGKRAKLISAREAAELVKSDDWVDYGFALGQPDLFDQAL
                     GARASQLQRVKIRGALTVRPRAILDADPGGEHFLWYNWHFSGYDRRQHDAGRCNYIPM
                     NFGEAPDYYRRFVEPIAVACVKTAPIDDNGYFNFGPSTSYHRAMIERAQVVIVETNTN
                     VPRVVGPDTGLHASEVDFVIDGGSAALPELPAGQVSAVERQVARLIAQEIQDGACLQI
                     GIGGMPNAVCSLLREANVRDLGIHTEMMVDGIGELIEMGIVTNRRKQIDTGRSVYSFA
                     LGTSKLYRLLDGNPAIWSYSVDYTNLPVNIAQNEHVVSINNTTQIDLQGQAASESSGH
                     RQISGTGGQLQFVRGAYASRGGKSFICLSSTCEVKGQRVSRIVSTLTPGNIVTTPRTD
                     VMYVVTEYGMVNLKGKSVAERAQALISIAHPDYRETLTRAAREQQLIPKGY"
     gene            4790..5581
                     /locus_tag="VNZ06_12225"
     CDS             4790..5581
                     /locus_tag="VNZ06_12225"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006008.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWW21567.1"
                     /translation="MNANVQPPVASTDDSALVSVQARGPITWLTLNRAQKYNVLSAAM
                     IAELKATLEHIAEDRNVRVIVLAAGGRAFCAGHDLGEMGAEVGLDALRALFNACGDLM
                     LTIQRIPQPVIARVQGIATAAGCQLVATCDLAVASSDARFAVSGINLGLFCSTPAVAI
                     SRNMLQKPALEMLLTGDFIDAPTARERGLVNRVVPADQLDAEVEKLAGTIASKSADAV
                     ASGKRLFYQQRELTIEAAYRRAADVMAQNAILDETQKGIARFSKR"
     gene            5586..>6972
                     /locus_tag="VNZ06_12230"
     CDS             5586..>6972
                     /locus_tag="VNZ06_12230"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HWW21568.1"
                     /translation="MTLLADLVGTSQRVRATSSRLAKVRELVAFLRILSPDEVQIAVL
                     YLSGETPQGHNGLGFANLRSAAAASTAASTASLSIASTDQALEAIAAIRGAGSAARRA
                     QSLRDLLARATPDERQFLLPLLLGELRQGAQAGVMLDAIASAAALPAATLRRAAMHSR
                     SLGAVARAALFDGPLALAKFQLQLFAPVAPMLAQTAADVGEALRELGDEVIFEWKMDG
                     ARIQLHKRGDEVRLYTRALNDVTAALPEIVEMARSLSPQELMLDGEAIAFDAMERPLP
                     FQVTMRRFGRKLNVEELRHTLPIRAFFFDCLYINGHSIADGTTAERFRALEQAVPIAL
                     RVPRLVSSSEQAARNFYEAALAAGHEGVMAKSQQAPYEAGNRGASWLKIKRSHTLDLV
                     VIAVEWGSGRRTGKLSNLHLGALDPVTGEYVMLGKTFKGLTDEMLAWQTRELLAREIR
                     RDHYTVYVRPEL"
BASE COUNT         1332 a         2244 c         2131 g         1265 t
ORIGIN      
        1 ccaccacgat gtagccgcgc gcagcaaaga atgccgccag caagatgccc tcggcatttt
       61 ccgaattgct cagatcggcg atattgaagc tcttcgaagc ctgcgtaccg tgtgcataca
      121 acacgatcgg gcgcggaccg ctgcagctcg cgccgccgcc gctcggtacc atcaacgcac
      181 ccgacgccgt ggtcgtttca ttctgtccac cgacggtcac gtattccacg tgatagacgt
      241 tgatgtcgca ttgaggaaag ccggcgagag tgagcacact cgtcagcacg gtttgcgcgc
      301 cgcccgcggt ggcatccgcg gtcagtcgcg ccgccagggt ctgagcagtg atcgacatcg
      361 tcaatgtggg cggggtctgc agcaacgcac cgcgccccaa gcccgaggag ccagaattgc
      421 cgtgctggcc gttgtcaccc ccgccgcagg cgctcacaaa cgacgcggtc agagcgaaca
      481 tcatcaaaac agacggtttg cgcttcatgg ccgcctaccc tgccgcagcg tggccagcac
      541 ccacaaggga aagaccgagc gaaacgctgt aggttcagcc ctgcaaggcc cgccgcccca
      601 atcgggcaac caacgcgaac attatcaaag cgatggcgct gtcgagaacc tgccaggcgg
      661 gccagcccgg ctttcgtcaa gcgccacatc gcccgtgggg tcaacgccac aacgctcaag
      721 caagcccgga cgcacgctgc ccgtcaatct ttactggcag aatgcccgcc tgccggtccc
      781 cctgctcgac cgcctgacac gcgctgtcat tgcggcggcg ccagtgtggc ttgccgtttg
      841 aaccggtatt gacggtgtgg gcactcaccc gtagaggttt gcgcgagaga tgaaatacct
      901 gccggcggtc accctgttgc tcgggctcac cagcgtgtca ggggccgagg tctacaatct
      961 ggacaccacg gcgacgcgcg tcggtttcga tattgagcac tttggcctgc actgggtcag
     1021 cgcgcacttc cgcagctttc agggtgactt cgtattcgac cgcaccggct cggccagccg
     1081 cgtggacgtc atcgtccaga cagactccgt cgattgcggc gactcgcact ggaaccccca
     1141 tctgcgctcg cccgagtggc tcgatgtaca gcagttccca ctgatgacct atcgctcccg
     1201 gcacatcgag ttcgaagggg gagaccgggc tgaggcgagt ggcgacctga cgcttcacgg
     1261 tgtcacgcac cccgtggtac tggatgtcag tcagctgcgc tgtaatggcg atggcaccga
     1321 ccaggcgtgc cggttcgtcg cgcacgcccg cgtcaagcgc tccgactatg gcctgccgca
     1381 cgggttctgg accggcggcg atcaagtgga cataacgatc gtcggcgtcg gcatgcacca
     1441 ctgacgcaaa gccccgcgct ggcgccataa ttgccaggcc gctaggaagc gataacaaca
     1501 agggggcgcg atgcgtggcc tgatgatgga tatgccgatg ctgatttcga ggctgatctg
     1561 ccatgccgac cgtgagcacg gcgacaccga gatcgtcagc cgtacggtcg aaggcccgat
     1621 ccatcggtac acctttcacg atgcgcacca tcgttcgcgc cagttggcca acgcgctgct
     1681 gacccaggga gtcagattcg gagaccgcat cgccacgctc gcgtggaaca cctaccgcca
     1741 cttcgagttg ttctatgcga tctcagggat cggtgcgatc tgccacacgg tcaatccgcg
     1801 gctgttcgcc gaacaactgc tctacatttt caatgacgcg gaagatcgct acatcttcct
     1861 cgatctcacg ttcgtcgaga tcgtgcagag gatcgcgccc cagctgccgc acgtgcgcgg
     1921 cttcgtcatt ctgaccgatc gggctcacat gccgcagaca tcgctgccca acgcgctttg
     1981 ctacgaggag ctggtgggtg ccgcatcgga tcagtttgag tggccgcagt tcgacgagca
     2041 caccgcctca tcgctgtgct acacctcagg caccaccggg aatccgaagg gagttctcta
     2101 cagccatcga tccaacatcc tgcacgcgta cgcgatggca ctgccggata cctactgcct
     2161 gtcggcgacc gacatcatgc tgcccgccgt gccgttgttc catgccaatg cctggggtat
     2221 tccgtatgcc tgcgccctgg tcggcacgaa aatggtattg cccggcagtc agctcgacgg
     2281 cgccagcctg catgaactgt tcgagcgcga gggtgccacg gtcaccgcag gcgtacccac
     2341 ggtgtggttc gggttgctgg ggtacatgaa gcaacatgga ctgcggttca gcacgctgaa
     2401 ccgcgcgctg atcggcggat cggcctgtcc ggccgcaatg atccagacat tcgaggagga
     2461 atacggcgtg cgggtactgc acgcatgggg tatgaccgaa ctgagcccac tcggtacgct
     2521 gacggctttc aaggcgaaac acacgcagca atccgcggcc gagcggcgcg cattgcagag
     2581 caaacagggc cgcgtgattt tcggcgtgga gatgaagatc gtggacgatg ccggcaacga
     2641 gttgccacgc gacggacgca ctttcggcaa tctgatggtc agggggccgt ggatttgcca
     2701 gcactacttc aaggctgagc acgtccagat cctgcaggat ggctggttcg gcacggggga
     2761 tgtcgcgacg atcgatccgg acggatacat gcaaatcacc gatcgcgcca aagatgtgat
     2821 caagtcgggc ggtgaatgga tcagctcaat cgagctggaa aactgcgccg tcggacaccc
     2881 ggacgtcgcg gaggcggccg tggtgggcgt accgcacgcg aagtggggtg agcgtccgct
     2941 gctgctgctg gtgcgcaaac caggcgcctc gccttcgcgc gaggacgtgc tgcagtacct
     3001 cgcccccaga atcgccaagt ggtggctgcc ggatgatgtg gtgttcgtgg acgcgctgcc
     3061 gcacaccgcg accggcaaac tgctgaagac caaactgcgc gaggactttc gcgatcatct
     3121 gctcaagcag accgaccggc cggccactca gccgcggtga ccgagtactc gcgcgggcaa
     3181 catgaggatg gcccgcagga atgcaaacag cgcatcgaac gtcaggccga ccagccggaa
     3241 cggcagcgtc agcagaaata cgatcggcca caggatcagc gcgaacagcg ccaccggcca
     3301 acacaccaca aacaacaaac accacaccaa taaggtcagc aatttcatgg tcagtacccc
     3361 ttcgggatga gctgctgctc gcgagccgcc cgcgtcagcg tctcgcgata gtccggatgc
     3421 gcgatcgaaa tcagtgcctg tgcgcgctcg gcgaccgact tgcccttcaa atttaccatg
     3481 ccatattcgg tcaccacata catcacatcg gtccgtgggg tggtgacgat gttgccgggc
     3541 gtcagcgtcg acacgatccg gctgacgcgc tgacccttca cctcgcaggt ggacgacaga
     3601 cagatgaaag acttgccacc tctggacgca tacgccccgc gcacgaattg caactgtcca
     3661 ccggtgccgc tgatctggcg atggcctgaa gactcagaag ccgcctgccc ctgcagatcg
     3721 atctgcgtgg tgttgttgat cgacaccaca tgctcatttt gcgcaatatt caccggcaga
     3781 ttggtgtagt ccaccgaata ggaccagatg gccggattcc catccagcag tcgatacagt
     3841 ttcgatgtgc cgagcgcgaa cgaataaacg cttcgcccgg tatcgatctg tttgcgacga
     3901 ttggtgacga ttcccatttc gatcagctca ccgataccgt cgaccatcat ctccgtgtgg
     3961 atgccaaggt cgcgtacgtt cgcctcgcgc agcagcgaac acaccgcgtt gggcatgcca
     4021 ccaataccga tctgaaggca cgcaccgtcc tggatctcct gcgcaatgag ccgcgcgacc
     4081 tgtcgctcga ccgcactgac ctggccggcc ggcaactccg gtagcgccgc acttcctccg
     4141 tcgatcacga agtccacttc gctcgcgtgc agaccggtat ccggcccgac cacacgtggt
     4201 acgttggtat tggtctcgac gatcaccacc tgcgcgcgct cgatcatcgc acggtgatag
     4261 gaagtgcttg gcccgaagtt gaaatagccg ttgtcatcga tcggcgccgt cttcacacag
     4321 gccactgcga tcggctcgac gaagcggcgg tagtagtccg gcgcttcccc gaaattcatc
     4381 ggaatgtagt tgcaacgccc ggcatcgtgc tggcgacggt cgtaacccga gaagtgccag
     4441 ttgtaccaca ggaagtgctc accgcccgga tccgcatcca ggatcgcgcg cggccgcacc
     4501 gtgagagcgc ctcgaatctt gacgcgctgc agctgtgacg cgcgtgcgcc cagggcctga
     4561 tcgaacagat cgggctgccc gagcgcaaac ccatagtcca cccagtcgtc cgacttgacc
     4621 agttcggccg cttcccgcgc gctgatgagt tttgcccgct ttccggactg tatcgtcgcc
     4681 acgaaccgcc cccgactggc tttggccggt ctttgcgaca atcaatttac cggcaaacgc
     4741 cgggcttgaa caggcaagct acaatgggct cctccactca aggtgtcgta tgaacgcaaa
     4801 cgtccaacca ccggtcgctt cgaccgatga cagtgcactg gtcagcgtgc aggcgcgcgg
     4861 accgattacc tggctcacgc tcaatcgagc ccagaaatac aatgttttat cggccgcgat
     4921 gatcgctgaa ctcaaggcga cgctcgaaca catcgccgag gatcgcaacg tgcgcgtcat
     4981 cgtactggct gcgggcggac gcgccttctg tgccggtcac gacctgggtg aaatgggcgc
     5041 ggaagtcggc ctcgacgccc tgcgcgcact gttcaacgcc tgtggcgatc tgatgctgac
     5101 gatccagcgc ataccgcaac cggtcattgc gcgcgtccag gggatcgcga ccgcggccgg
     5161 ctgccaactg gtagccactt gcgatctggc ggtggcgagc agcgatgcgc gctttgcggt
     5221 ctcgggtatc aatctggggc tgttctgttc gaccccggcg gtggccatct cgcgcaatat
     5281 gttgcaaaag ccggcgctgg agatgctgtt gaccggcgac ttcatcgatg cgccaaccgc
     5341 gcgcgaacgc ggtctcgtca atcgcgtggt gcctgccgat cagctcgacg cggaggtcga
     5401 aaaactcgcc ggcaccatcg cctccaagtc cgccgacgcc gtcgcctcgg gcaagcgcct
     5461 gttctatcag cagcgcgaac tgacgattga ggccgcttat cgtcgggccg cggatgtcat
     5521 ggcacagaac gcgatcctcg acgaaacaca gaagggcatc gcgcgcttct ccaagcgctg
     5581 aatcgttgac gctgctcgcc gatctcgtag gcacctcgca gcgcgtacgc gccacctctt
     5641 cgcgactggc gaaagtgcgc gaactggtgg cgttcctgcg gatcctgtcg cccgatgagg
     5701 tgcagatcgc ggtcctgtat ctgtcgggtg agacccctca gggccacaac ggcttgggtt
     5761 tcgccaacct gcgctcggcg gccgccgcca gtaccgccgc cagcacagct tcgctttcga
     5821 tcgcgagtac ggatcaagcg ctcgaggcga ttgccgccat ccgcggcgcc ggctcagcgg
     5881 cccgaagggc gcaatcgctg cgggatctac tggcgcgcgc gaccccggat gagcgccagt
     5941 tcctgctgcc gttgctgctg ggcgagctgc gccagggtgc gcaggccggt gtgatgctcg
     6001 atgccatcgc ctcggccgcg gcgctgccgg cagcgacact gcggcgtgcc gcgatgcatt
     6061 ccaggagcct gggtgcggtc gcccgcgcag cgctgttcga cggtccgttg gcgctcgcga
     6121 aattccagct ccagctgttc gccccggtcg caccgatgct tgcgcagacg gcggccgatg
     6181 tcggcgaggc cttacgtgag ctcggcgacg aggtgatctt cgaatggaag atggacgggg
     6241 ctcgcattca gctgcacaag cgcggtgatg aggtgcgcct ctacacgcgc gcgctcaacg
     6301 acgtgaccgc tgcgttgcct gagatcgttg aaatggcacg ctcgctttca ccgcaggagt
     6361 tgatgctcga cggcgaggcc atcgcattcg atgcgatgga gcgaccactg ccctttcagg
     6421 tcacgatgcg ccgcttcgga cgcaaactga acgttgagga attgcgccac acactgccga
     6481 ttcgggcgtt cttcttcgat tgtctctaca tcaatggcca cagcatcgca gatggcacga
     6541 ccgcggagcg ttttcgcgcg ctcgaacagg cggttcccat cgcactgcgt gtgccgagac
     6601 tggtcagctc atccgaacag gcggccagga atttctacga agccgcgctc gccgcgggcc
     6661 atgaaggcgt gatggctaaa tcacagcaag caccttatga ggccggcaat cgtggagcca
     6721 gttggctcaa gatcaaacgc tcgcatacgc tggatctcgt ggtgattgcc gtggaatggg
     6781 gcagtggtcg ccgcaccggc aagctgtcga atctgcatct gggtgcactg gatccggtca
     6841 ccggcgagta cgtcatgctc ggcaagacct tcaagggtct gaccgatgag atgctcgcgt
     6901 ggcagacacg cgaactgttg gcgcgcgaga ttcggcgcga ccactacacg gtgtatgtgc
     6961 gcccggaact cg
//
LOCUS       DATMRM010000404        17559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1815485, whole genome shotgun sequence.
ACCESSION   DATMRM010000404 DATMRM010000000
VERSION     DATMRM010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17559
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815485"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VNZ06_12235"
     CDS             <1..318
                     /locus_tag="VNZ06_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HWW21569.1"
                     /translation="TYALSLLKPGVCCRDVYAQHNAYLRRQNLSEERRLSIHGMGYDM
                     VERPLIRSDEQMSLEEHMAIVCHPGIYNERMFVHNTDIYLIEAQGVSECLHRTPKQIF
                     DLT"
     gene            complement(311..1525)
                     /locus_tag="VNZ06_12240"
     CDS             complement(311..1525)
                     /locus_tag="VNZ06_12240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW21570.1"
                     /translation="MSREFRIVTVMALGFGLVGIDRFLIATLYPVIARDLHLSYGDIG
                     VITGALALAWGVSALFTGNASDRLGRRLVLVGSLIVFSALIGASGLATGLAGLVAVRV
                     VMGFADGAYTPTSIAATLEASAIHRRGLNIGIQQMMLPLCGLGIAPLLVSWLLKVINW
                     RWVFLLFVLPGLALAYVVSRLFRAGTSNTSQRAHVQLRWRDWLDVLKYRNVVLGGVLM
                     LCWLTCLITTSAFLPNYFVDHLNLPFGQMSTVMSAIGLGSTVGTIALPWLSDATGRKP
                     IMVGSTIGALASLLMLAKTGVQPNTLFAWLFMVHFFNNALITLTVGPLCSETVPPALM
                     ATASGVVIAIGELVGGGLTPIVVGQIAQRIGIEHLLRVPCVAMVLGLLVSLLTRETRP
                     TARRASLLSAST"
     gene            1596..2432
                     /locus_tag="VNZ06_12245"
     CDS             1596..2432
                     /locus_tag="VNZ06_12245"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWW21571.1"
                     /translation="MVSIRGITFEENTRNAMGLEFYDLSHPWGLGQPCWPYFEDVKIE
                     RLHTMAKSGVLTQRITTVMHSGTHVDAPGHVVEGTPFVDEVPLPHFFGTGVVVAIPKD
                     EWGVITADDLERATPKIQPGDIVIVNTGWHKYYGDNQKYYGYSPGFYKEAGEWFAAKK
                     VKMIGTDTQALDHPLGTAIAPHGPGWPNGLLPFVCQDYKRRTGHDVLQDFPEWEPCHR
                     AILSAGICGFENVGGEIDLVTGKRVTFAAFPWRWTKGDGCIVRLVAIIDPTGKYRIET
                     GR"
     gene            2444..2791
                     /locus_tag="VNZ06_12250"
     CDS             2444..2791
                     /locus_tag="VNZ06_12250"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWW21572.1"
                     /translation="MHVKRLAQASPYKAPNHFEVSALRLQGFESAGPKSAWVGLSYFL
                     PGGGAGPDSSALEKTYVVLAGSITVRASGQEVTLGPMDSCYIAPNELREVRNLSNEVA
                     SMLVVMPYPEGSP"
     gene            2788..3591
                     /locus_tag="VNZ06_12255"
     CDS             2788..3591
                     /locus_tag="VNZ06_12255"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWW21573.1"
                     /translation="MTAASRREPASLFDIRNKVAVVTGASGAFGALAARVLAAAGARL
                     VLAAGRPAELEEVARQCRDAGAEVIAVARRPNAEQDCDAIMEAAKSGFGSLDILVVGS
                     GLNIVSPIVDMPPEKFLEVMNGNVTGAWLLCRAFGKVVVPQGRGGKVVLMSSARGALG
                     HPAGYSAYCASKAATDGLTRALGCEWGKHAICVNAIAPTVFRSALTAWMFEDDDKART
                     TRAGMLARIPVGRLGEPEDLAGPLLFLVSRASDFYTGHILYADGGYTAG"
     gene            3595..4509
                     /locus_tag="VNZ06_12260"
     CDS             3595..4509
                     /locus_tag="VNZ06_12260"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HWW21574.1"
                     /translation="MSSSTPPIAIIGAGLMGHGIAQVFALSAHPVRMFDPSASALSSV
                     HQRIAANLAALGQGQDALRHVSLHETLGNAVKDAHFVFECGPEDLSLKQALFAEIEAG
                     APASAILASNTSVIPITAIMRRVRDATRALGTHWWNPPYLIPLVEVIRTVRTSNESIE
                     RTLALLTSVGKTPVRVERDVPGFVGNRLQHALWREAIALVQAGICDARTVDTVVKASF
                     GRRLAVLGPLENADLVGTDLTLAIHNTVLPHLNRSDSALPLLRQLVSEGKLGMKSGEG
                     FRRWSQNDRAEISARLLRHLQRLGSDVS"
     gene            4532..5470
                     /locus_tag="VNZ06_12265"
     CDS             4532..5470
                     /locus_tag="VNZ06_12265"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HWW21575.1"
                     /translation="MARPTTNKVIITCAITGAIHTPTLSAALPYTPGDIAQQSLDAAR
                     AGAAILHLHARNPADGSPTGDPDVFAQFLPQIHAECDAVINLTTGGSPDMTVEQRLAA
                     ALRFQPEMCSLNMGSMNFAFHGLASASSDWKFPWERRYIEDSEAFIFRNTFRDIARVY
                     RLMGDFGTRYEHECYDIGHLYNLAHFLDKGLAKPPLWIQSVFGILGGIGADPDNVVFM
                     RRTADRLFGKDYVWSVLAAGRHQMPLTSQAAMMGGHVRVGLEDSLFIGRGKLAGSNAE
                     QVLKIRRILEELGFEIASPSDARQLLSLKGRQAVKI"
     gene            5528..6433
                     /locus_tag="VNZ06_12270"
     CDS             5528..6433
                     /locus_tag="VNZ06_12270"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWW21576.1"
                     /translation="MDLHRLDLNLLVALDALLAERSITRAAERLHLSPSATSGALSRL
                     RTYFEDDLLKQEGRRMVPTPLGETLQARVRDCLLHVQATVDVRPQFDPGSSTRKFTLM
                     MSDYVSAVLMPDVIARVQREAPGVVIELIANSQPWAALDRGDADFLVIPENFTRREHP
                     SETLFEDEFICVCWNENPLISDSISAEQFLKLSHVVARIGTERPPTIDAWFFERYGHV
                     RRVAVVAMNFTSVPQLLIGTPRIALMHRRLAITVAKSSPIKLLKAPFEMPKLVEVIQW
                     HRYRDADPGRKWLHGLLKSSAAAAH"
     gene            6396..7865
                     /locus_tag="VNZ06_12275"
     CDS             6396..7865
                     /locus_tag="VNZ06_12275"
                     /inference="COORDINATES: protein motif:HMM:NF044822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21577.1"
                     /translation="MVCSSHRPPQPTSDRSRASVVLDIGKSVSKASLWTRDGRLLERR
                     TRTNARIHLHSYDALDATGIEYWLADALRDFGRIAEVAAIIPVAHGAAAGIVQGDHLA
                     VPAFDYESSIPDAVRREYDPLRDAFALTGSPALPHGLNLGAQLYYQQSLQPRLLEQRA
                     LILPWPQYWSWLLSGTAASEVTSLGCHSDLWRPRDAAPSHLSVSRGWASQVAPLRAAG
                     EVLGALSSHWCARTGLSHDVQVHCGLHDSNAALLAARGYPQIADQDATVLSTGTWFVA
                     MRSLIDADSFDMHSLPEDRDCLVNVDVQGKPVPSARFMGGREIELLTGADGFRIELPA
                     HQAALLSAVARVLSSATVLLPSFAPGSGPYGSGSGTWVSAPTDPYERAAAICLYIALL
                     ADVSLDLIGSKHALLVEGRFAQCQLLVRALAALRPGSAVYVARAQHEVAFGALRVLHS
                     ALGRDDSLVRVTALDDDLIDYRCRWRQLVTARSLSLDRR"
     gene            7882..9969
                     /locus_tag="VNZ06_12280"
     CDS             7882..9969
                     /locus_tag="VNZ06_12280"
                     /inference="COORDINATES: protein motif:HMM:NF006189.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HWW21578.1"
                     /translation="MMISIQNQWDPAYAATLSPEQLLLYRSNLLGADLRITNYGGGNT
                     SAKLVGKDPVSGQQIPVLWVKGSGGDLGTMGLDGFATLYQDKLLALQTHYRGPEDEDR
                     MVGLYPHCAFNLNPRAPSIDTPLHALIDRPHVDHMHPDAVIAIAASSDSRQLTEQIWG
                     QQIGWLPWIRPGFELGMQLNRFLQAHPQARGVLLEAHGLFTWGNCAHSCYENTLDIIN
                     RAIAWLEARSDATLAFGPSRVTALPPDRRRDLAAALMPRIRGLICGDTPKIGHFDDSA
                     EVLQFVNSRKLEALAAMGTSCPDHFLRTKIRPLVLAYDATQENERDLLERLPQAIATY
                     RQQYQDYYERCKRGFSPKMRDPNAVVYLVPAVGMITFAADKATARIASEFYVNAINVM
                     RGAAAVSTYRGLPEQEAFDIEYWPLEEAKLQRLPPRQALAGRIALVTGGAGGIGMASA
                     RRLAAEGACVVLCDIDPAALAQAQSELRAQFGADSAIGVHADVTNERTINDLFRETAW
                     RYGGLDICVSNAGIASASPIEDTTLELWNRNLSILTTGYFLVSRASVRLLKAQGLGGS
                     IVFIGSKNALAASAGAAAYCTAKAAELHLARCLALECAGDNIRVNVVNPDAVLRGSRI
                     WQGEWAEQRARQNNTTPEALEKLYRERSLLKASVYPEDVAEAVAFFASNRSAKSTGNI
                     INVDAGNATAFTR"
     gene            10010..11302
                     /gene="rhaI"
                     /locus_tag="VNZ06_12285"
     CDS             10010..11302
                     /gene="rhaI"
                     /locus_tag="VNZ06_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose catabolism isomerase"
                     /protein_id="HWW21579.1"
                     /translation="MTDPTNPQFIRDSNAQALAAHQADYDALGAQLQRLGRDIESVVD
                     AVSRFAVAIPSWGVGTGGTRFARFPGPGEPRNVYEKLEDCAVIQALTRATPAVSLHLP
                     WDRTGDYAQLREVASGLGLAFDAMNSNTFQDQTGQPHSYKFGSITHTDRATREQAIAH
                     NIDCIQAGKQLGSRALTLWVGDGANFPGQHHLTRAFERYLESAKQIYAALPPDWTLLL
                     EHKMYEPAFYASVIQDWGTSLLAAQSLGPQARCLVDLGHHAPNVNIEMIVARLIYAGK
                     LGGFHFNDSKYGDDDLDAGSIEPFRLFLVFDQLVDAQYRRVSSFSPAYMLDQSHNVTD
                     PIESLMSSAIELQRAFAQALLVDREALSQHQHSNDVLLAAQTLKRAFTLDVHPILCEA
                     RRRRGGAIDPIHTYRRSGYRAARAQARPAVAGSGSGIV"
     gene            complement(11311..12108)
                     /locus_tag="VNZ06_12290"
     CDS             complement(11311..12108)
                     /locus_tag="VNZ06_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HWW21580.1"
                     /translation="MHSTEREALILDSLKHKGFISFRELERRVKASPATIRRDLARMS
                     TAGLITRVRGGAMLGETASSGSASAQASLGLQGVPFHENIHRHRLQKQAIGRAAAKLC
                     KSGQGVMIDGGSTTLQMCRHLQGKTMQVLTNSLHIVSALLAQPGTRVLMPGGQVFREQ
                     NIVLSADGDDVMPKFHAPQLFMGAASMGPAGLMQADILLIAAERRLIERAEQIIVLVD
                     SSKFEGHSGHVVCRLEDIDIVVTDSAIASKHVRMLEKAQVKTIIARD"
     gene            12189..13925
                     /locus_tag="VNZ06_12295"
     CDS             12189..13925
                     /locus_tag="VNZ06_12295"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HWW21581.1"
                     /translation="MKYLTRLPLALITAGLCPLAQAAEWTSGSATAAHCEQLAPQMSE
                     RWPDRSTRVISVKWQPAGPIMVMNLGGPPSAIAAPDHCELIGEMQSRSGANGQHYAIR
                     FHLRMPAQWNRRFYFQGGGGSNGVLGDALGRYSQSAAPALLQGFAVVSQDSGHDNQTN
                     NDASRGGEITFGFDQTARVNYGHASLPRVADAAKAAIRQFYGASAAHSYFVGCSKGGQ
                     EALAAAERYGREFDGIASGDPAMSLPRAAIAEAWNTQTFARLVRAQPGSPITVDQLQT
                     VMTNEDFERLRAAVLSACDAADGVSDGIVGDFEHCTTQHVWPALEALKCAAGQSDHCL
                     SSAKIDALVRVIKGPVDSHGKALYSDFPWDASIAAPGWRMWNLGSVMPPIPILNVVLG
                     GPSLATVFTTPPTTINGNPPSALQFLLQFDFDKDAPKIYASDAAFPTSGWDNVSARSA
                     DLSLFRAHHGRLIVFHGSADPIFSINDTIAWWNEVDRGNGGRASGFVRLFPVPGMNHC
                     FGGDATDQFDVLAPLVDWVERNKAPEQIMASADPMSPWPKRTRPLCPYPRIARYRGTG
                     DVDTASSFECRR"
     gene            14132..15136
                     /locus_tag="VNZ06_12300"
     CDS             14132..15136
                     /locus_tag="VNZ06_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21582.1"
                     /translation="MDIRSRRNAMIAATILAAGLASAQSPPPAPAPLDLAKMRADISE
                     YRKKPDTPGTGQYPAIKEMVASLPNHVIYRPADLSKFGPRKLGVLAWGNGGCAADGAG
                     ARFHLSEIASHGYLAIAAGTILSGPGAPAGAEPLQPALPGAPGPGQPGFSPPPLATQA
                     SSLREAIDWALSENERSGSPYYHRINPKWIAVSGWSCGGLQALQVASDPRVQTVVIHN
                     SGIFPAGASPIKGLDIDKGALKKLHTSIIYILGGPTDIAYANGMDDFKGIDSVPAMVV
                     NTNVGHQGTFLEANGGRAASVAVSWLDWQLKGDQQAAQRFVGKDCGLCKDAKWTVQRK
                     "
     gene            complement(15137..16345)
                     /locus_tag="VNZ06_12305"
     CDS             complement(15137..16345)
                     /locus_tag="VNZ06_12305"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HWW21583.1"
                     /translation="MRIATLARCAALGLVLFTGVTDAHTKSTSYSEWNVEGASVRLVY
                     TVPLVESARLKRADETQPADARLALYLAAHLSVTAGTQPCSPSPPRVLQASAQFRRVE
                     IDYRCAGSAHIVLHSSAFFDLVPSHVSLAQIRLSDGRVIQQIFTRDSQTLATSGDANS
                     ARDAGILRYIQMGIVHIFTGTDHMAFLLGLVLISRRPRDLIFVVTGFTMGHSITLALA
                     VTGIIRPHAQYIDALVALTIAMIGAENIAVATHRPGVLAAWLGSSLLLMAAASALGTG
                     TMPALVLLGAGLFAANYLMLAGHLPDAARLRIVVTVVFGLIHGFGFAANLLEQRLPSA
                     QLLHILFGFNLGVEIGQLTLVLGVLALVALLVRLKLALPRPIVVETVSAGLVGLGTYW
                     FISRAFAPPS"
     gene            complement(16342..17250)
                     /locus_tag="VNZ06_12310"
     CDS             complement(16342..17250)
                     /locus_tag="VNZ06_12310"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWW21584.1"
                     /translation="MTEQAAVFDPAQASARRSMLLLSLGALLGLAVAGYGLFTAKGTR
                     SRSVPPEAVAIVNQRPILRSDFMTQTQVQFQVPYAQSTAEQRHRVLQDMINEELMVQR
                     GLEMDLPSYDPDVRSALVAGVELEVTADVLARQPTEDELRQYYAAHSDRYSTEGVMRL
                     RDLVTADPTVAAAAVSELRAGKPLDAVMLRHALKDSGKFMKAGQVDTDDLYQFAVRAK
                     LDDPLFQAAQSLQSGQVSDPIKQADGVHLLFMVAHRLAVAQSFEQSRARVWNDLKQQA
                     QSQVREANLRYLRGRADILVAADFPQ"
     gene            complement(17247..>17559)
                     /locus_tag="VNZ06_12315"
     CDS             complement(17247..>17559)
                     /locus_tag="VNZ06_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWW21585.1"
                     /translation="QSLAHRPYDAQAAERGRGVFGGNGNCYDCHAGDARGVPEIGAPA
                     LTGPTFLYGGDGNTEFRSVYFGRHGQCPAWINRLTALQVRALAVYLVSTAEAATSGGH
                     G"
BASE COUNT         3320 a         5634 c         5462 g         3143 t
ORIGIN      
        1 acctacgccc tgtcgctgct gaagccgggt gtgtgctgcc gcgacgtcta cgcgcagcac
       61 aacgcttacc tgcgccgaca gaatctgtcc gaagagcgac gcctgagtat ccacggcatg
      121 ggatatgaca tggtcgaacg accgctgatt cgctcggatg agcagatgtc cctcgaagag
      181 catatggcca tcgtctgcca tccgggcatc tataacgaac gcatgttcgt gcacaacacc
      241 gacatctatc tgatcgaggc tcagggcgtc tccgaatgtc tgcaccgcac gcccaagcag
      301 atcttcgatc tcacgtagac gcggacaata gcgaggctct gcgggccgtc ggccgcgtct
      361 cgcgcgtcag caggctgacg agcagcccaa gcaccatcgc cacgcacggc acccgcagca
      421 gatgttcgat gccgattcgc tgcgcgatct gacccacgac aatcggcgtc agccctccgc
      481 caaccaattc tccaatggcg atcacgacgc cggaggccgt ggccatcagc gccggtggga
      541 ctgtctccga gcacagcggg ccgaccgtca gcgtgatgag cgcgttgttg aagaagtgaa
      601 ccatgaagag ccacgcgaac aaagtgttcg gttggacccc ggtcttcgcc agcatcagca
      661 gtgacgccaa ggcgccgatg gtcgagccca ccatgatcgg cttgcgtccg gtcgcatccg
      721 acaaccaggg caacgcgatc gttccaacgg tcgagcccag tccgatggcg gacatcacgg
      781 tgctcatctg gccaaacggc aggttcagat ggtccacgaa atagttggga agaaacgcgc
      841 tggtcgtgat gaggcacgtg agccagcaca gcatcagcac gccaccgagt acgacgttgc
      901 gatacttcag tacatcgagc cagtcgcgcc agcgcagttg cacgtgagcg cgttgcgatg
      961 tgttggaagt gccagcgcga aacagtcgcg acacgacgta ggcgagcgcc agccctggaa
     1021 gcacgaatag caggaacacc cagcgccagt tgatcacttt cagcagccac gacaccagca
     1081 gcggtgcaat ccctaggccg cacaacggca acatcatctg ctgaatgccg atattcagcc
     1141 cgcgacgatg gatggccgat gcctccagcg tcgcggcaat gctggtcgga gtgtaagcac
     1201 cgtcggcaaa gcccatcacg actcgaaccg cgaccagccc cgcaagcccg gtggccagtc
     1261 cgctggcgcc aatgagcgca ctgaacacga tcagtgagcc caccagtacc aggcgacggc
     1321 caagacggtc cgatgcattg ccggtgaaca gcgccgacac accccatgcc agcgccagcg
     1381 ccccagtgat gacgccgatg tcgccatagc tcaggtgcag atcgcgggcg atcaccgggt
     1441 acagggtcgc aatcagaaag cggtcgatgc ccaccaggcc aaagcccagc gccatgaccg
     1501 tcacgatcct gaactcacgt gacacgcaac cccctgcccg cattgtggac ttgtagtacc
     1561 accatacgat acgctctggc gacaaggagt gcgatatggt cagcatccgt ggcatcacat
     1621 tcgaggaaaa cacccgcaac gcaatggggc tcgagttcta tgacttgagt catccctggg
     1681 gcctgggaca gccgtgctgg ccgtatttcg aagacgtgaa gatcgagcgg ctgcacacca
     1741 tggcgaagtc cggcgtgctc actcagcgca tcaccacggt gatgcactcc ggcacgcacg
     1801 tcgatgcgcc gggacacgtg gtcgaaggca cgccattcgt ggacgaagtg ccgctgccgc
     1861 atttttttgg aaccggcgtg gtagttgcca ttccgaagga tgaatggggc gtcatcactg
     1921 ccgacgatct ggagcgcgca acgcccaaga ttcagcccgg cgatatcgtc atcgtcaaca
     1981 ccggttggca caaatactac ggcgacaatc agaagtacta cggctattca cccgggttct
     2041 acaaggaagc cggtgaatgg tttgcggcca agaaagtcaa gatgatcggc accgacaccc
     2101 aggcgctcga tcatccgctg ggcacggcca ttgcaccgca cggccccggc tggcccaacg
     2161 gcctgctgcc gtttgtctgc caggactaca agcgccgcac cggtcacgac gtgctgcagg
     2221 acttcccaga atgggaaccc tgtcatcgcg cgattctgtc agccggcatc tgcggctttg
     2281 agaatgtcgg cggcgaaatc gatctcgtga ccgggaaacg ggtcacgttc gcggcattcc
     2341 cgtggcgctg gaccaagggg gacggttgca tcgtgcgcct ggtcgcgatc atcgatccga
     2401 cgggcaagta tcgcatcgag accggacgct aggagccgag ctcatgcacg tcaaacgact
     2461 cgcgcaggcc tcgccgtaca aggcgcccaa ccacttcgag gtgagcgcgt tgcggctgca
     2521 gggcttcgag agcgcaggtc cgaagagtgc gtgggtggga ctatcctact tcctgccggg
     2581 cggcggcgcg ggtccagact cctctgcgct ggagaaaacc tacgtcgtgc tggccggctc
     2641 gatcacggtc cgggccagcg gacaggaagt cactctcggt ccgatggaca gttgctacat
     2701 tgcgccgaac gaactgcgtg aagtgcgcaa tctcagcaac gaggtcgcga gcatgctggt
     2761 ggtcatgccc tatccggaag gctccccatg accgcggcca gccgaaggga gcctgcctcg
     2821 ctgttcgaca tccgcaataa ggtcgcggtc gtgaccggcg cttcgggcgc ctttggtgct
     2881 ctggctgcac gggtgcttgc ggcggccggt gcgcgactgg tgctcgccgc gggccgaccg
     2941 gcggaactcg aggaggtggc acgacagtgc cgcgatgcgg gcgctgaggt gattgcggtc
     3001 gcgcgccgtc cgaatgccga gcaggattgc gacgccatca tggaagcggc caagagtggc
     3061 ttcggatcgc tcgacattct ggtggtgggc tccgggctga atatcgtctc tccgatcgta
     3121 gacatgccgc cggagaagtt tctggaagtc atgaacggca atgtcaccgg tgcctggctg
     3181 ctgtgccgtg ccttcggcaa ggtcgtggtt ccgcaggggc gaggcggcaa agtcgtactc
     3241 atgtcatcgg ctcgaggagc gctgggtcat ccggccggat acagcgctta ctgcgcttcc
     3301 aaagcggcga ccgatggttt gacgcgtgcg ctgggctgcg agtggggcaa gcacgccatc
     3361 tgcgtcaatg cgatcgctcc cactgttttt cgctctgcac tgacggcgtg gatgttcgag
     3421 gacgacgaca aggcgcgcac cacgcgcgcc ggcatgctgg cgcgcatacc cgtggggcgc
     3481 ctcggagagc cggaggatct tgccggaccg ttgctgtttc ttgtctcacg cgcatcggat
     3541 ttctataccg ggcacatcct gtatgcggac ggcgggtaca ccgcgggctg acccgtgagc
     3601 tcatcgacgc cccccattgc gatcattggc gccggactca tgggacacgg catcgcgcag
     3661 gtattcgcgc tgtcggctca tccggtgcgg atgtttgacc cgtcggccag cgctttgagc
     3721 tcggtgcatc aacgtatcgc cgctaacctc gccgcgctcg gtcaaggtca ggatgcgcta
     3781 cggcatgtgt cgctgcacga aacccttggc aatgcggtca aggacgcgca tttcgtattc
     3841 gaatgcgggc cggaagatct gagcctcaag caggcactgt ttgccgagat cgaggccggt
     3901 gcgccagcga gcgcaatcct cgccagcaac acctcggtca tcccgatcac cgccatcatg
     3961 cgcagagtcc gcgatgcgac gcgtgcgctg ggcacccact ggtggaaccc accgtacctg
     4021 attccgctgg tcgaagtgat tcgcacggtg cgcacctcga acgagtccat tgaaaggacg
     4081 ctggctttgc tgacaagtgt cggcaagaca ccggttcgcg tcgagcgcga tgtccccggg
     4141 ttcgttggca accggctgca gcatgcgctg tggcgcgagg cgattgcctt ggtgcaggcc
     4201 ggtatctgcg atgcgcgcac ggtcgacacc gtcgtcaagg cgagcttcgg acgacgcctc
     4261 gccgtgctgg gtccgctgga aaacgcggat ctggtcggca ccgacctgac gctggcgatc
     4321 cacaacaccg tattgccgca tctcaaccgg tccgacagcg ccctgcccct gctgcggcaa
     4381 ttggtcagcg agggcaagct tggcatgaag tccggcgagg gcttccggcg ctggagccag
     4441 aacgatcggg ccgagatcag cgcacgcctc ctgcgacatc tgcaaaggct tggaagcgat
     4501 gtgagctaag cggccgacgg ccgggggcac tatggctaga ccaaccacca acaaagtcat
     4561 catcacctgc gccatcacgg gcgcgataca cacgccaacg ctgtcagcgg ctttgcctta
     4621 cacgccgggc gacatcgcac agcaatcact cgacgcagcc cgcgccggcg cagccattct
     4681 gcacctgcat gcgcgcaacc ccgccgacgg ctcccccacc ggggacccgg acgtcttcgc
     4741 gcagttcctg ccgcaaatcc atgccgagtg cgatgcggtc atcaacctga ccaccggtgg
     4801 cagtcccgac atgaccgtcg agcaacgcct ggccgcggcg ctgcgctttc agcccgagat
     4861 gtgctcgctc aacatgggtt cgatgaactt cgccttccac ggcctcgcga gcgcatccag
     4921 cgattggaag tttccgtggg agcgcaggta catcgaagac agtgaagcct tcatctttcg
     4981 caacaccttt cgtgacatcg cgcgcgtcta ccgattgatg ggcgatttcg gcacgcgcta
     5041 cgagcacgaa tgctatgaca tcggccatct ctataacctt gctcacttcc tcgacaaggg
     5101 cctggccaag ccgccgctgt ggattcaaag cgtgttcggg attctcggtg ggatcggcgc
     5161 cgatccggac aacgtcgtgt tcatgcgtcg caccgccgat cgtctgttcg gcaaggacta
     5221 tgtctggtcg gtgctggccg ccggacgaca tcaaatgccg ctcacctctc aggccgcgat
     5281 gatgggcggt cacgtgcgtg tgggactgga ggactcactg ttcatcggac gcggcaagct
     5341 cgccggcagc aacgctgagc aggtgctcaa gatccggcgc attctggaag aactcggctt
     5401 cgaaatcgcg agcccgagcg acgcgcgaca actgctgtcg ctgaagggta gacaggctgt
     5461 caaaatctaa gcgcgataca ctgccggcac gcggctcgcg ccgctggcag cagaggtgag
     5521 tgggggtatg gatctgcatc gcctggattt gaatctattg gttgcgctcg acgcattgct
     5581 ggcggagcgc agcatcacac gcgccgccga gcggctgcac ctgagtcctt cggcgaccag
     5641 tggggcgctg tcgcggctgc gcacctactt tgaggatgat ctgctcaaac aggaaggccg
     5701 gcgcatggtc ccgactccgc ttggggagac gctgcaggcc cgtgtacgag actgtctgct
     5761 gcacgtgcag gcaaccgtgg atgttcggcc gcagttcgac ccgggcagct ccacgcgaaa
     5821 attcacgctc atgatgtccg actacgtctc ggcggtgctg atgccggacg tgattgcgcg
     5881 ggtgcagcgt gaggcacccg gggtcgtgat cgagctgatc gccaacagcc agccgtgggc
     5941 cgcactcgat cggggcgacg cggacttcct ggtcattccc gaaaacttca cccggcgaga
     6001 gcacccgtcc gagacactgt tcgaggatga attcatctgc gtgtgttgga acgagaatcc
     6061 gctgatcagc gacagcatct cggccgaaca gtttctgaag ctcagccacg tcgtcgcccg
     6121 cattggcact gaacggccgc cgaccattga cgcctggttc ttcgagcgct acggacacgt
     6181 gcgtcgtgtg gcggtggtgg cgatgaattt cacatcggtg cctcagttgc tgatcggcac
     6241 gccgcggatc gcattgatgc atcgccgttt ggcgatcacg gtggcgaaat cctcgccgat
     6301 caagctgctg aaggcgccgt ttgaaatgcc gaagctggtg gaggtgatcc agtggcatcg
     6361 ctaccgcgac gctgatcccg gcaggaagtg gctgcatggt ctgctcaagt catcggccgc
     6421 cgcagcccac tagcgatcgc agccgggcca gcgtggtgct ggacatcggc aagagcgtat
     6481 caaaggccag cctgtggacc cgggacggca ggctgctcga gcgtcgcacc cgcaccaacg
     6541 cgcgcatcca tctgcactcg tacgacgcat tggacgccac aggcatcgag tactggctgg
     6601 ccgatgccct ccgggacttt gggcgcatcg ctgaagtcgc ggcgattatc ccggtggcac
     6661 acggcgccgc tgcggggatt gtgcagggcg atcatctcgc ggtgcctgcg ttcgactacg
     6721 agagctccat tcccgacgcg gtacgccgcg aatacgaccc gttgcgcgat gcgtttgcgc
     6781 tcaccggctc acccgcgctc ccccacggcc tgaacctggg agcgcagttg tactatcaac
     6841 agtcgctgca accgcgacta ctcgagcagc gcgccctcat cctgccgtgg ccgcagtact
     6901 ggagttggct gctgtcaggg accgcggcgt cggaggtgac cagcctggga tgtcacagcg
     6961 atctgtggcg gccgcgcgac gcagcgccct cgcacctgtc ggtctcgcgc ggttgggcat
     7021 cgcaggtggc gccgctgcgt gccgccggag aggtcctcgg agctctgagt tcgcactggt
     7081 gcgcgcgcac cggactgtca cacgatgtac aggtgcattg cggactgcat gactccaacg
     7141 cggcgctgct cgccgcgcgc ggctacccgc agatcgcgga tcaggatgcc acggtgttgt
     7201 ctaccggcac ctggttcgtc gcgatgcgca gtctcatcga tgcagacagt ttcgacatgc
     7261 acagcttgcc cgaggatcgc gattgcctgg tgaacgtcga cgttcaggga aagccggtgc
     7321 cctccgcacg cttcatgggc ggccgcgaga tcgagctgct gactggtgcc gatggctttc
     7381 gcatcgaatt gcccgctcac caagccgcac tgctgagcgc ggtggctcgt gtcctgtcat
     7441 ccgccacggt gctgttaccc agcttcgccc ctggaagcgg tccctacggg agcggatccg
     7501 gcacctgggt gtctgcgccg accgatccct acgaacgagc ggcagcgata tgcctgtaca
     7561 ttgcactgct ggccgatgtc tcactggatc tgattggatc caagcacgcg ctattggtgg
     7621 aaggccgctt cgcgcagtgc cagctcctgg tgcgcgcact cgccgcgctc cggcccggca
     7681 gtgcggtcta tgtggcgcgg gcgcagcacg aggtcgcatt tggtgcactg cgcgtgctgc
     7741 actccgcgct gggacgagac gattcgttgg tgcgggtcac tgcgctagat gacgatctga
     7801 tcgactaccg ttgtcgctgg cggcaactcg tgaccgctcg gtccttgtcg cttgatcgaa
     7861 gatgatcaat actgatcatt aatgatgatt tcgatccaga atcaatggga tccggcgtat
     7921 gccgcgacgc tctcgcccga gcagctactg ctctatcgct ccaatctgct gggcgcggac
     7981 ctgcgcatca cgaactacgg cggtggcaac acgtcagcaa aactggtcgg caaggatccg
     8041 gtcagcggtc agcagatccc cgtgctatgg gtaaaaggtt ccggcggaga tctcggcacg
     8101 atgggcctgg atggatttgc aacgctctat caggacaagc tgctggcgct gcagacgcac
     8161 taccgcggcc ccgaggatga agaccgcatg gtcgggctct accctcattg cgccttcaat
     8221 ctcaatccgc gcgccccgag tatcgacacc ccgctgcacg ccttgatcga tcgcccgcac
     8281 gtcgatcaca tgcatccgga tgcggtgatc gcaattgccg ccagcagcga cagccgccag
     8341 ctcactgagc agatctgggg ccagcagatc ggctggctgc cgtggatccg cccgggcttc
     8401 gaactgggca tgcaactcaa ccgctttttg caggctcatc cgcaggcacg cggcgtcctg
     8461 ctcgaggcgc acggactgtt cacctggggc aactgcgcgc atagctgcta tgagaacacg
     8521 ctcgacatca tcaaccgcgc catcgcctgg cttgaggctc gttccgatgc gacgctcgcc
     8581 tttggtccca gccgagtgac tgcgctcccg ccggatcgtc gtcgagacct ggcggccgcg
     8641 ctgatgccac ggattcgcgg gctgatctgc ggtgacacgc caaagatcgg ccatttcgat
     8701 gacagcgccg aggtgctgca gttcgtgaac agtcggaagc tggaagctct ggcggcgatg
     8761 ggtacctcct gtcccgatca ctttcttcgt actaagattc ggccgttggt gctggcatac
     8821 gacgccactc aggagaacga gcgcgatctg cttgagcgct tgccgcaggc gattgcgacc
     8881 tatcgccagc agtaccagga ctattacgag cgctgcaagc gcggcttttc accgaaaatg
     8941 cgcgatccga acgctgtggt ctatctcgtc cccgcagtcg ggatgatcac gttcgcggcg
     9001 gacaaagcca ccgcgcgcat cgccagcgaa ttctacgtca acgcgatcaa tgtcatgcga
     9061 ggcgccgccg cagtctccac ctaccggggt ctgcccgaac aggaagcctt cgacatcgaa
     9121 tactggccgc tcgaggaagc caagctccag cgcctgccac cgcgccaggc actggccggc
     9181 cgaatcgcgc tggtcaccgg tggtgcgggc ggcatcggaa tggccagcgc ccggcggttg
     9241 gccgccgagg gtgcgtgcgt cgtgctctgc gatatcgacc cggcggccct cgcccaagcg
     9301 cagagcgaac tgcgtgcgca gttcggggcc gacagtgcca tcggcgtcca cgccgatgtg
     9361 accaatgagc gcacgatcaa tgatttgttt cgcgaaacgg catggcgcta cggcgggctc
     9421 gatatctgcg tctccaatgc gggcattgca tccgcctcac cgatcgaaga cacgacgctc
     9481 gaactgtgga accgcaacct gagcattctg accaccggct atttcctggt gtcgcgcgcc
     9541 agcgttcgtc ttctgaaggc acagggactc ggcggttcca tcgtcttcat tggatcgaag
     9601 aatgctctgg ccgcatccgc gggtgccgcc gcctactgca ccgcgaaggc cgccgagctt
     9661 caccttgcgc gctgcctggc gttggagtgc gccggcgata acatccgcgt caacgtcgtc
     9721 aatccagacg ccgtgctcag aggatccaga atctggcagg gcgagtgggc cgaacaacgc
     9781 gcccggcaaa acaacaccac acccgaagcg ctcgagaagc tctatcggga acgcagcctc
     9841 ctgaaggcca gcgtgtatcc ggaggatgtg gcggaggccg tggccttctt tgcttcgaat
     9901 cgttcggcca agtccacggg caacattatc aatgtggacg ccggcaatgc aacggccttc
     9961 acgcgttagg gcatcgggct gaaaccggtt cgggtttccg ggggaaaatg tgaccgatcc
    10021 aaccaatcct caattcatcc gcgacagcaa cgcccaggcg ctggcggcac atcaggccga
    10081 ctatgatgcg ctcggtgcgc aactgcagcg cctcgggcga gacatcgaat ccgtggtgga
    10141 cgcggtcagc aggttcgcgg tcgcgattcc ctcctggggc gtgggtaccg gcggcacgcg
    10201 cttcgcacgt ttcccgggac ccggggaacc tcggaacgtg tacgagaaac tcgaggactg
    10261 cgccgtcatt caagccctga cgcgcgcgac gcccgcggtg tcattacatc tgccgtggga
    10321 tcgcaccggg gactatgcgc agttgcgcga agtcgcgagc gggctggggc ttgcgtttga
    10381 tgcgatgaat tccaatacct ttcaggacca gacaggacag ccgcactcct acaagttcgg
    10441 cagcatcact cacaccgacc gggccacgcg cgaacaggcg atcgcgcaca acatcgactg
    10501 catccaggcg ggcaagcaac tcggatccag ggcgttgact ctctgggtcg gcgatggtgc
    10561 caacttcccc ggtcaacatc atctcacgcg cgccttcgag cgctacctgg agtccgccaa
    10621 gcagatctac gcggctttgc cgccggattg gacgctgctg ctggaacaca agatgtacga
    10681 accggcgttc tacgccagcg tgattcagga ttggggcacc tcgctactgg cggcgcagag
    10741 tctgggaccg caggcgcgat gcctggtcga cctgggtcat cacgcgccga acgtgaacat
    10801 cgagatgatc gtcgctcggc tgatctatgc cggcaagctg ggcggcttcc acttcaacga
    10861 ctccaagtac ggcgatgacg atctggacgc gggttccatc gaacctttcc gactgttcct
    10921 ggtgttcgat caactggtgg acgcgcagta ccggcgggtc tccagcttct caccggccta
    10981 catgctcgat caatcacaca acgtgaccga tccgatcgag agcctgatga gcagcgcgat
    11041 tgaactgcag cgagcgtttg cacaggccct gctggtcgac cgtgaggccc tgtcgcagca
    11101 ccagcacagc aacgatgtgc tgctggcggc gcaaacactc aagcgcgcct tcactctcga
    11161 cgtccatccg attctgtgcg aagcgcggcg acgccgtggc ggagccattg atcctatcca
    11221 cacctatcga cgcagcggct atcgcgccgc ccgcgctcaa gcgcggccag cggtcgctgg
    11281 tagcggcagc ggaatcgtct aaagtcgcct ctagtcacgg gcgatgatcg tcttcacctg
    11341 agccttctcc aacattctca catgcttcga agcgatggca gagtcagtga cgacgatatc
    11401 gatgtcctcg agccgacaca cgacatgccc ggagtgaccc tcgaacttcg agctgtcgac
    11461 cagcacgatg atctgctcgg cgcgctcgat cagacgtcgc tcggcggcaa tcagcaggat
    11521 atccgcctgc atgaggccag cgggccccat cgacgccgct cccatgaaca gctgcggcgc
    11581 atgaaacttg ggcatcacat catcgccgtc ggcggacagc acaatgttct gttcgcgaaa
    11641 cacctgccct cccggcatca gaacccgggt tccgggctgc gccagcagcg cactgacgat
    11701 gtgcaaggag ttggtcagca cttgcatggt cttgccttgc agatggcgac acatctgcag
    11761 ggtggtggac ccgccgtcga tcatcacgcc ctgccccgac ttgcagagtt ttgctgccgc
    11821 gcgaccgatc gcttgcttct gcaggcgatg gcgatggata ttctcgtgaa acgggacacc
    11881 ctgcagaccc agactggctt gcgccgacgc gctgccggac gaggcagtct cgccaagcat
    11941 cgctccaccg cgcacgcgcg tgatcagtcc ggccgtgctc atgcgcgcca ggtcgcgtcg
    12001 gatcgtcgcc ggcgatgctt tcacgcgtcg ctcgagctcc ctgaaggaaa tgaagccctt
    12061 gtgcttcagg ctgtcgagaa tgagcgcttc acgctcagtc gaatgcatgc tcgctccgcc
    12121 tgtttgagac gcggctactg tggccggtgt cgccgggcaa ttcaatcaaa atcgctcacg
    12181 gcaggaccat gaaatatctg acgcgattgc cactcgcact catcacggca ggattgtgcc
    12241 cgctggcgca ggctgccgaa tggacgtccg gcagcgccac tgcagcgcat tgcgaacagc
    12301 tcgcgccgca gatgagcgag cgctggccgg atcgcagtac gcgggtgatc tcggtcaaat
    12361 ggcagccggc agggccgatc atggtcatga atctgggtgg acctcccagc gctatcgcgg
    12421 cacccgatca ctgcgaactg atcggtgaga tgcagtcacg cagcggtgcc aacggtcagc
    12481 actacgcgat tcggtttcat ctgcgcatgc ccgcacagtg gaaccggcgc ttctactttc
    12541 agggtggagg cggaagcaac ggcgtgctgg gcgatgcgtt gggccgatat tcgcagagcg
    12601 cagccccggc cctgctgcag ggcttcgcgg tggtcagcca ggattcgggc cacgacaatc
    12661 agacgaacaa tgatgcatcg cgcggtggcg aaatcacgtt cggcttcgat cagactgccc
    12721 gggtcaacta tggccacgca tccttgccgc gggttgccga cgccgccaaa gccgcgatcc
    12781 gccagttcta tggagcttca gccgctcact cctatttcgt cggctgctcg aaaggcggtc
    12841 aggaggcgct ggccgctgcc gaacgctatg gaagagaatt cgatgggatc gcctccggag
    12901 atccggcgat gtcgctgcca cgcgcggcga tcgcggaggc ctggaacacg cagaccttcg
    12961 cccgactggt cagagcgcaa ccgggcagcc cgatcacggt cgatcaactg cagaccgtca
    13021 tgaccaacga ggacttcgag aggctccgcg cagcggtctt gagtgcgtgt gatgccgccg
    13081 acggcgtcag cgacggcatc gtgggcgatt ttgaacattg caccacgcaa cacgtgtggc
    13141 ctgccctgga ggcgctcaag tgtgccgccg ggcaatcgga tcattgcctc agcagcgcca
    13201 agatcgacgc gctggtacgc gtcatcaaag gcccggtcga cagccatggc aaagcgctgt
    13261 actcggattt tccgtgggat gcgagcattg ccgcgccggg ctggcgcatg tggaatctgg
    13321 gctcggtgat gccaccgata cccattctca acgtcgtctt gggtggcccc tcactggcga
    13381 ccgttttcac cacaccgccg accacgatca atggcaaccc accctcggcg ctgcagttcc
    13441 tgctgcagtt cgactttgat aaggatgcac cgaagatcta cgcctctgat gccgcgttcc
    13501 ctacctcagg ctgggacaat gtctcggccc gttcggcgga tctgagcctg ttccgggccc
    13561 accacggcag gctcatcgtg ttccacggta gtgccgatcc tattttctcg atcaacgaca
    13621 cgattgcctg gtggaacgaa gtggaccgtg gcaacggcgg ccgggcgtcg ggcttcgtca
    13681 ggctatttcc cgtgccgggt atgaatcatt gcttcggagg cgatgcgacc gatcaattcg
    13741 acgtgctggc gcccctggta gactgggtcg aacgcaacaa ggcgcccgag cagatcatgg
    13801 cgtccgccga tccgatgtca ccctggccca agcgaacacg acccctgtgc ccgtatccgc
    13861 ggatcgcccg ctaccgaggc accggcgatg tcgatactgc ttcctcgttc gagtgccgcc
    13921 gatagtgcgc gttccgaagt tcgaatttgt ctacgagtgc gacgtgacgc tcgctggggg
    13981 ctgcggtgct gagtcgccta ttcgaagtgg tctgataccg ccatcccagg cacgttgctc
    14041 gtttcggtgc tgccgaaagc taccctcgtg aaaaacggat tctcaatcgg cgcgcgggcc
    14101 actaccgtat cggaaaatac aggggaccct catggacatt cgatcgcggc gcaacgccat
    14161 gatcgctgcg acgattctcg cggcgggact cgcgagcgct cagagcccgc cgcccgcacc
    14221 cgctccgctc gacctggcga agatgcgcgc ggacatttcc gaataccgca agaagcccga
    14281 cacgccgggt accggacaat accctgcgat caaagaaatg gttgcctcac tgccgaatca
    14341 cgtcatctac cggccggcgg atctttccaa attcggcccg cggaaactgg gcgttctcgc
    14401 ctggggcaac ggcggatgcg cagcggatgg cgccggagcg cgctttcatt tgagcgaaat
    14461 cgcctcccac gggtacctgg cgatcgctgc cggaaccatc ctcagtggtc ccggtgcacc
    14521 tgcgggcgcg gaaccactac agcccgcact ccccggcgca cccggtcccg ggcaacccgg
    14581 gttctcgccg cctccgctgg cgacacaggc atccagcttg cgtgaggcca tcgactgggc
    14641 cctgtcggaa aacgaacgtt caggcagtcc ctactaccac cgcatcaacc cgaagtggat
    14701 cgcggtgtcg ggctggagct gcggcggtct gcaagcgctt caggtcgcga gcgacccgag
    14761 agtgcaaacg gtggtcattc acaacagcgg catctttccc gccggtgcgt caccgatcaa
    14821 aggcctggac atcgacaagg gcgctctgaa gaaactgcat acctcgatca tctacatcct
    14881 gggtggcccg acggatattg cctacgccaa cggcatggac gacttcaaag ggatcgattc
    14941 cgtgccagcg atggtcgtca acaccaacgt cgggcaccag ggcacgtttc tggaagccaa
    15001 cggaggtcgt gccgcctcgg tcgcggtcag ctggctagac tggcagttga agggtgacca
    15061 gcaagccgcc caacgcttcg tcggcaagga ctgtggcctg tgtaaggacg cgaaatggac
    15121 cgtgcagcgc aagtgactag ctcggcggag caaacgcgcg gctgatgaac cagtaggtgc
    15181 cgagcccgac cagacccgct gatacggttt ccaccacgat cggtcgcggc aacgccagtt
    15241 tcaatcgaac cagcagggcg accagtgcca gcacccccag taccagcgtg agctgcccga
    15301 tctccacgcc aagattgaag ccaaagagga tgtgcagcag ttgggcggaa ggcagccgtt
    15361 gctcgagcag attggctgcg aacccgaagc catgaatcag gccaaacacc accgtcacga
    15421 cgatacgcag gcgtgccgcg tccggcagat gccccgcaag catcaggtag ttcgcggcga
    15481 acaggcccgc gccgagcagc accagtgcgg gcatggtgcc ggttcccaac gcactggcag
    15541 cggccatcag cagcagtgag ctacccagcc acgccgccag cacgcctggc cggtgggtcg
    15601 ccacggcaat gttctccgca ccgatcatgg cgatggtgag cgccaccagc gcgtcgatgt
    15661 actgcgcgtg cggccggatg atgccggtca ccgccaatgc gagcgtgatg ctgtgaccca
    15721 tggtgaagcc ggtgacgacg aatatcaggt cgcgcggccg ccgggagatc aacaccagac
    15781 ccagcaggaa agccatgtga tcggtgccgg tgaagatgtg cacaatgccc atctgaatgt
    15841 agcgcagaat tcccgcatcc cgcgcgctat tcgcgtcgcc ggaggttgcc agagtctggc
    15901 tgtcccgggt aaagatctgc tggattaccc tgccgtcgct caggcggatc tgcgccagcg
    15961 agacatgtga cggcaccagg tcaaagaacg ctgacgaatg cagcacgatg tgcgctgagc
    16021 ccgcacatcg ataatcgatc tcgacgcgcc ggaattgcgc gctcgcctgc agtactcggg
    16081 gcggggaagg ggaacacggc tgtgtacctg cagtcaccga caggtgcgcg gcgaggtaga
    16141 gggcgagtcg tgcgtccgcg ggttgcgttt catctgcacg tttgagtcgc gcggattcca
    16201 ccagcggcac ggtatagacc agccgcaccg acgcaccctc gacgttccac tcagaatagc
    16261 tcgtgctctt ggtgtgcgcg tcagtgactc cggtgaacag caccaggccc agggctgcgc
    16321 accgcgccaa cgttgcgatc ctcattgagg aaagtccgcc gccaccagaa tgtcagcgcg
    16381 accgcgcaga taccgcagat tggcctcgcg cacctggctc tgtgcctgct gtttgaggtc
    16441 gttccacacc cgtgcccgcg actgttcgaa actctgggca actgccagac gatgcgcaac
    16501 catgaacagc agatgcaccc cgtcggcctg tttgatcgga tcggagacct gcccactctg
    16561 cagcgactgc gcggcctgga acaagggatc atcgagtttg gcgcggaccg cgaactggta
    16621 cagatcgtcg gtatcgacct gcccggcctt catgaatttg ccggagtctt tgagcgcatg
    16681 ccgcaacatg accgcatcga gtggcttgcc tgctcgtagc tccgagacgg ccgcggcggc
    16741 cacagtcgga tcggccgtga cgaggtcgcg cagccgcatg acgccctctg tcgaatagcg
    16801 atcgctgtga gccgcgtagt actgccgcaa ctcatcctcg gttggctgcc gcgccagcac
    16861 gtctgccgtc acttccagtt cgacgcccgc aaccagcgcc gagcgcacgt ccggatcgta
    16921 agacggcaga tccatttcga gtccacgctg caccatgagc tcttcgttga tcatgtcctg
    16981 cagcacccgg tgacgttgct cggcagtgct ttgcgcgtac ggcacctgga actgcacctg
    17041 cgtctgagtc atgaagtcgc tgcgcaggat cggtcgttga ttgacgatgg cgaccgcttc
    17101 gggcggtacg ctgcgcgaac gcgtaccctt cgccgtgaac agtccgtacc cggcgacggc
    17161 cagtcccagc aatgcgccga ggctcagcaa cagcatcgag cgtcgcgcac tcgcctgcgc
    17221 cggatcaaac accgccgctt gctcggtcat ccatgtcctc cggacgtcgc agcctctgct
    17281 gtgctgacaa ggtagacggc cagggctcgc acctgaagcg ctgtcaggcg attgatccac
    17341 gccgggcatt gcccatgtcg tccgaaatag acggagcgaa actctgtgtt gccgtcgccg
    17401 ccgtagagaa acgtgggccc ggtgagcgcc ggagccccga tctcggggac gccacgcgca
    17461 tcgcccgcat gacagtcgta gcagttgccg ttgccgccga agacgccccg accgcgttcg
    17521 gcggcctgag cgtcataggg tcgatgtgcc aggctctgc
//
LOCUS       DATMRM010000405         2864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2405386, whole genome shotgun sequence.
ACCESSION   DATMRM010000405 DATMRM010000000
VERSION     DATMRM010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2405386"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VNZ06_12320"
     CDS             complement(<1..537)
                     /locus_tag="VNZ06_12320"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HWW21586.1"
                     /translation="MNDRLINGRPTDCIDSNDRGLHYGDGLFETISCLNGRPRWLEFH
                     LERLQRGLARLRIPFPEPDVLRSEIAALSSVHERCVIKVIVTRGVATRRGYAPSGDET
                     PTRMVSRHPWPASAGTVGEFRVGVSAVCLSASPTLAGLKHLNRLEQVMAQMECADRQL
                     AEVLMLTATRHVISGSMSN"
     gene            complement(534..1955)
                     /locus_tag="VNZ06_12325"
     CDS             complement(534..1955)
                     /locus_tag="VNZ06_12325"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF006563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HWW21587.1"
                     /translation="MFNEVHSGGLLVQPAAVTAQTLRQLAYHDPEHFPVLLDSAAVGP
                     LSRYSILAAYPQSALWLDAHGVLQSSGSLEVAAGQGFLTALENAWRRESRIGQHVSDC
                     GMPFRGGWIVFLGYELAGEVEPRLALPSGGATALEGPGAFALRVPAGVIYDHVESRAW
                     MFAEGHALQLLPALEAALRTVREDTVASEPVSVLLREEPAERFQSRVEAALEYIAAGD
                     IYQANLSRPWRGEWVVAGGTQAVACDLYERLRRVNPSPFAALAQFQDWRLLSCSPERL
                     VRVTGSRVETRPIAGTRPRSGIDDRHALEIAGLIEHPKERAEHVMLIDLERNDLGRIC
                     RAGSVRPDEFMAIESYAHVHHIVSNVSGELRSDLTPIDVLRAVFPGGTITGCPKFRCM
                     QIIAELEGEARQAYTGALGYLNHDGSMDFNILIRTLALQAGRLEFRTGAGIVADSDWQ
                     RELAETRAKARGILHGLNADTPP"
     gene            complement(1989..>2864)
                     /locus_tag="VNZ06_12330"
     CDS             complement(1989..>2864)
                     /locus_tag="VNZ06_12330"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWW21588.1"
                     /translation="GRNPRKISPFFVSSTIINMISGHLSIRYGLRGPNLGVVTACTTS
                     THALGIGMRTIQYGDADMVLAGGSEMATTPTGLGGFCQAKAMSTRNDSPQTASRPWDR
                     DRDGFVLADGGGAIVLEDLEHAKKRGACIYGELIGFGMSGDAHHMTSPPEDGDGARRA
                     MVSALRDAQINPDQVQYMNAHATSTQLGDSAETIAIKNAFGDYAKKLAVSSTKSMTGH
                     LLGAAGVLEAIFSVLAIRDQVAPPTINLDNPGPDCDLDYVPHTARQMKIDITASNSFG
                     FGGTNGTLVFRRFSG"
BASE COUNT          540 a          944 c          862 g          518 t
ORIGIN      
        1 attcgacatg ctgccactga tcacgtgccg cgtcgcggtc agcatcagca cttctgccag
       61 ttgtcgatcg gcgcactcca tttgcgccat cacctgctcc aggcggttca gatgtttgag
      121 acccgcaagc gtgggactgg cggacaaaca caccgccgac acgcccacac gaaattcacc
      181 caccgtgcct gccgacgccg gccatgggtg gcggctgacc atgcgcgtcg gcgtttcatc
      241 tccgctggga gcatagccgc gccgcgtcgc aacgccacgc gtaacgataa ccttgatgac
      301 acagcgctcg tggaccgaag acagcgccgc gatctccgaa cgcagtacat cgggttccgg
      361 gaagggaatc cgcagccgcg ccagcccacg ctgcaggcgt tcgaggtgga actccagcca
      421 acgaggccga ccgttcaggc agctgattgt ttcgaacagc ccgtcgccat agtgcagtcc
      481 acggtcattg ctgtcgatgc agtccgtcgg ccgtccgttg atcaggcggt cgttcacggc
      541 ggcgtatccg cgttcagtcc atgaagaatc ccgcgcgctt tggcccgagt ttcagccagt
      601 tcgcgctgcc aatcggaatc cgcgacgatc ccggcaccgg tccgaaactc gagccgcccc
      661 gcctgcaatg cgagcgtgcg gatcagaatg ttgaagtcca tcgacccgtc atgattgaga
      721 taccccagtg cgcccgtata ggcctgcctg gcctcgcctt cgagttccgc gatgatctgc
      781 atgcagcgga atttggggca cccggtgatc gtgccaccgg ggaacaccgc ccgcagcaca
      841 tcgatcggtg tgagatcgct gcgtagctcg ccgctgacgt tcgagacgat atgatgcacg
      901 tgggcgtagc tctcgatcgc catgaactca tctggacgca cgctccccgc ccggcaaatg
      961 cgtccgagat cattgcgttc cagatcgatc agcatgacat gttcggcccg ttctttcgga
     1021 tgctcgatca accctgcgat ttcgagcgca tgccggtcgt caatcccgct gcgcggccgc
     1081 gttcccgcga tcggccgggt ctcgacccgg cttccggtga cgcgtaccag tctctccggt
     1141 gagcagctca gcagtcgcca gtcctggaat tgagccagcg cggcaaacgg cgacggattg
     1201 acgcgccgaa gtctttcata caagtcgcag gcgacagcct gtgtgccacc ggcaacgacc
     1261 cattcaccgc gccacggtcg ggaaagattc gcctgataga tatcgcccgc cgcgatgtat
     1321 tccagcgcag cttccacgcg ggactggaag cgttcggccg gctcttcccg cagtagcacg
     1381 ctgaccggct cactcgcgac cgtatcctcg cgcaccgtgc gcagcgcggc ttccagtgcc
     1441 ggcaataact ggagtgcatg cccttcggca aacatccagg ctctggattc gacgtgatca
     1501 taaatcacgc ccgccggcac ccgcagtgcg aacgcacccg gcccctccag cgcggtcgcg
     1561 ccgccagatg gaagagccag gcgaggctcg acttcgccgg cgagttcata gccgagaaat
     1621 acgatccagc caccgcgaaa tggcattccg caatctgata cgtgttgccc gatcctggat
     1681 tcgcggcgcc aggcgttctc cagcgctgtg agaaagccct gaccggcagc gacttcgaga
     1741 ctgccactgg attgcagcac gccgtgcgcg tcgagccaca gagcgctttg cggatacgcc
     1801 gccagaatcg aatagcggct caatgggccg acggctgcgc tatcgagcaa caccggaaag
     1861 tgctcggggt cgtgataggc gagctgccgc agcgtctgcg cggtcacggc ggcaggctgc
     1921 acgagcagcc cgccggaatg aacctcgttg aacatcgatg ccgctcgagt aactgcggcc
     1981 ggccagagct agccgctgaa gcgacgaaat accagcgtgc cgttggtgcc gccgaacccg
     2041 aaagagtttg aggccgtgat gtcgattttc atctgtctgg cggtatgcgg cacgtagtcc
     2101 aggtcgcagt ccggacccgg attgtcgaga ttgatcgtcg gcggcgccac ctgatcgcga
     2161 atggccagca cgctgaatat tgcctcgagc acacccgcgg caccgagcag gtgcccggtc
     2221 atcgacttgg tggagctcac tgccagcttc ttcgcgtagt cgccaaacgc gttcttgatc
     2281 gcgatggtct cagcgctgtc acccagctgc gtggaggttg cgtgcgcgtt catgtactgc
     2341 acctgatcgg ggttgatctg cgcatcacgc agagccgaga ccatggcgcg tcgggcacca
     2401 tcgccatcct ccgggggaga ggtcatgtgg tgggcatcgc cactcatgcc aaatccaatc
     2461 aactcgccat agatacacgc gccacgcttc ttcgcgtgct ccagatcctc gagcactatc
     2521 gcgccgccgc catccgccag cacaaagccg tcgcgatcgc gatcccacgg gcgactggct
     2581 gtctgcggcg agtcattacg ggtggacatc gctttggcct ggcagaaacc accgaggccg
     2641 gtgggggtgg tcgccatctc cgaaccgccc gccagcacca tgtcggcgtc gccgtattga
     2701 atagtccgca taccgattcc caacgcgtgg gtcgaggtgg tacatgccgt caccacaccc
     2761 aggttcgggc cgcgcagccc gtagcgaatt gagagatgcc ctgaaatcat gttgatgatc
     2821 gtggaggaca cgaagaacgg agagatcttg cgcgggttac gccc
//
LOCUS       DATMRM010000406         5186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1484236, whole genome shotgun sequence.
ACCESSION   DATMRM010000406 DATMRM010000000
VERSION     DATMRM010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484236"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VNZ06_12335"
     CDS             complement(<1..588)
                     /locus_tag="VNZ06_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21589.1"
                     /translation="MLQWAFYALVVAALISLSGWLIEQALRRRQLPTRWAWVAAMSLT
                     VLLAYLSLPVSIAPRAPQPRLLRESWTAIQRVAPLGLPPLPFASVAAARHTGTARGNA
                     LRFDTLLIDSWFGLSGLLALSLALCGARVSWSRRRWPLRLVESHCVGVARNVGPAVVG
                     LRYPTIVIPRWVLDRPAAEQQLILAHEASHLTARDP"
     gene            complement(599..958)
                     /locus_tag="VNZ06_12340"
     CDS             complement(599..958)
                     /locus_tag="VNZ06_12340"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HWW21590.1"
                     /translation="MPHQLTDREADIMNVLWELGPSTVAEVRAALKSRFAHTTVQTML
                     RILESKGFVAHQEEGRQHRFRPLVQEGTARQSALKHLLAKMFRGSSELLLTQLVSDQK
                     LTAEQVRRMRKLLSKKE"
     gene            complement(1075..2073)
                     /locus_tag="VNZ06_12345"
     CDS             complement(1075..2073)
                     /locus_tag="VNZ06_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21591.1"
                     /translation="MTRRAALPIETWALLYFLSYAPYAVLVRWLASVPYPAFHRALTG
                     LEILPVTTILSGVFTFLFAWLSGWSRHAHRIHIAGASFPFPTAWTFASGIGTALLLFT
                     VPLSFTFQGVSIPYMQLLMRGDVLLIAPLVDVLAGRRVRWYSWAALGLVALGLSLTIH
                     ARGGLRLPPLALLTVLLYTVGYFVRLAVMTRIAKSGDPDMVKRYFVEEKIVAIPLAVL
                     MLALLSLVNFGTQSGQLGFGFIGVWSSGQMTPILLLSLLLFVISVFSILILIDQRENT
                     FCVPIERSASILAGIAAAYVLAQLSLGSLPTNAEMAGALLLIAAIVLLSLGASRAR"
     gene            complement(2083..3267)
                     /locus_tag="VNZ06_12350"
     CDS             complement(2083..3267)
                     /locus_tag="VNZ06_12350"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWW21592.1"
                     /translation="MTAAREPQDFDPLIAESFDSPHEVYRELRARCPVAHSDAWGGFW
                     ALTRYAEVQQALQQSSVFITSVQNVVPRLAFTGRRPPLHLDPPEHTPYRRALNPLFKP
                     ERIARLEPKIRALAASLLQAMIARGGGDLCSDYSSHLTIRVFAEWMDVPESMVPDLQQ
                     VGRAYNIAVQSANGEMVRETSLKLYDMARAMIASRRQEPRDPATDPTAALLAARIDGA
                     PFPDEMILGTIRQVLVVGIIAPTIVVGSIAVHLARDPPLQQRLRADLSAMPRAIEEFL
                     RLYTPYRGFARTANRDVEINGRAIREGEPIALLYASANRDEAVFEHADQFDMNRPNID
                     QHLAFGMGPHMCAGMPLARLELRIAVEELLKHTRGFELSGEPTQTRFPEIGALSVPLR
                     FL"
     gene            3331..4863
                     /locus_tag="VNZ06_12355"
     CDS             3331..4863
                     /locus_tag="VNZ06_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21593.1"
                     /translation="MESLRHCARTGPRFGIVLGLLALNPVAGLAQNDSYDGANWWQSG
                     SGQTLGASAQYDDALGRVGLLNAAGAIDTRGHPFFEPIGGNGRACVTCHQPANAMSSS
                     AAAIRARWQATNGRDPLFAAVDGSNCPSAPQELESSHSLLLRHGLFRIYLPWPPKAAD
                     GSTITPEFDLELVSDPSGCNSDPVYGLHSAHPQVSVFRRTRVVANMKYVTGGGGAAFN
                     IKDGSPMARDPETGRFVNMNLLSDARAPTLKTQAIDAAMTHLQASAAPSAAQLLSIVQ
                     FEDQVYVAAEFSSKGGSLTEPGAPQDLGPAAMFKEQPGLGDNFYQPVFGYFDQWAGLS
                     GADPQSQFRASIARGMQVFFTRPFFIRDVTHLNTVGLGNPIRRSCATCHNARMTGMDF
                     APGWMDLGTGNKPWADDLPMTSELPLFKLTCKASAPSHPFLGPVIYTHDPGRALISGR
                     CIDIGAITMQQFRGLAARAPYFSNGSADDLRALVEYYDKRFEAHYTEQEKQDLINFLS
                     VL"
     gene            4860..>5186
                     /locus_tag="VNZ06_12360"
     CDS             4860..>5186
                     /locus_tag="VNZ06_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21594.1"
                     /translation="MSSQLSDRAFRLAVATALALLASCLAAAADPGREIVFVGCPILR
                     NTELPCWLGESGGELYYLGAQGDLTSQFYPPQFDHRMLVEGIVSDEARICGGRVLKPV
                     KVSVLPQ"
BASE COUNT          974 a         1639 c         1703 g          870 t
ORIGIN      
        1 cggatcgcgt gcggtgagat gcgatgcctc gtgggcgagg atcagttgct gctccgcggc
       61 gggtctatcc agcacccagc ggggtatcac aatggtgggg tagcgcagac cgaccacggc
      121 tggaccgacg ttgcgggcaa cgccgacaca atgcgactca accagccgca ggggccagcg
      181 gcggcgcgac caggagaccc gtgcgccgca cagcgccagc gacagggcca acaaccccga
      241 gagcccgaac cacgaatcga tgagcagcgt atcaaagcgc aatgcattcc cgcgcgcggt
      301 cccggtgtga cgagcggcgg cgacagaggc aaagggcagc ggcggcaaac cgagcggtgc
      361 aacgcgctga atggccgtcc agctctcacg cagcagcctc ggctgcggcg ctcttggcgc
      421 aatcgagacc ggcagcgaca gatacgcgag cagcacggtg aggctcatcg ccgcgaccca
      481 tgcccaccgg gttggcagct ggcgccgacg cagggcctgc tcgatcagcc agccggataa
      541 cgatatcagt gctgcgacga ccagtgcgta gaacgcccat tgcagcatgg gatgaccctc
      601 actccttctt ggagagcagc tttcgcatgc gccggacctg ctcagccgtg agcttctggt
      661 ccgagacgag ctgagtgagc aacagctccg aggatccgcg gaacatcttt gccagcagat
      721 gcttcagggc gctctgtcgt gccgtgccct cctgcaccag tgggcggaaa cggtgctgcc
      781 ggccttcttc ctggtgcgcg acgaaaccct tggactcgag gatgcgaagc atggtctgga
      841 cagtggtatg agcgaagcgg ctcttcagcg ccgcgcgcac ctcggccact gttgacggcc
      901 ccaattccca aagcacgttc atgatgtcgg cttcgcggtc cgtgagctga tggggcatgt
      961 gcgtctgacg tcatctccta aggtgttagg agattagcga tggcatcggc gcatgtcaac
     1021 taaaccttta ggagatggcc ccacccactg actttcgccc agctcgcgca gccactagcg
     1081 ggctctgctc gctcccagcg acagcagcac gatggccgcg atcagcagca gcgcgccagc
     1141 catctcggcg ttggtgggaa gcgagccgag cgacagctgg gccaggacgt aagcggcggc
     1201 gataccggcc agaatactcg cggagcgctc gatggggacg cagaacgtgt tctcgcgctg
     1261 atcaatcaga atcaggatcg aaaacaccga gatcacgaac agcagcagcg acagcagcag
     1321 aatcggggtc atctgcccgc tgctccacac gccgatgaat ccgaagccga gctggccgct
     1381 ttgcgtgccg aagttcacca gcgacagcag cgcgagcatc agcaccgcca gcggaatcgc
     1441 gacgatcttc tcctcgacga agtagcgctt gaccatgtcc ggatcgccgc ttttcgcgat
     1501 ccgcgtcatg accgcgagtc gcacgaagta acccacggta tacagcagca ccgtgagcag
     1561 cgcgagcggc ggcagcctca ggccgccacg tgcatggatg gtcagtgaca gtcccagcgc
     1621 gaccagtccc agcgcggccc acgaatacca gcgcacccgg cgcccggcca gcacatccac
     1681 cagcggggcg atgagcagca cgtcgccacg catgagcaat tgcatgtagg gaatgctgac
     1741 accctggaac gtgaacgaca gcggcacggt gaacagcaac agcgcggtgc cgatgccgga
     1801 ggcaaaggtc caggccgtcg gaaacgggaa ggatgcgccc gcgatgtgga tgcggtgagc
     1861 gtgccgcgac cagccgctga gccatgcgaa caggaacgtg aagacgccac tcaggatcgt
     1921 cgtcaccggc aggatttcca ggcccgtgag cgcgcgatga aacgctggat acggcacgct
     1981 cgccagccag cgcaccagca ccgcgtacgg tgcatagctc aggaaataca gcagcgccca
     2041 ggtctcgatc ggcaatgcgg cccgtcgcgt catcgcccgc tactaaagaa agcgcagcgg
     2101 taccgacagc gcgccgatct ccggaaagcg ggtctgcgtg ggttcgcccg acaattcgaa
     2161 tccacgggtg tgcttgagca gctcctcgac cgcaatgcgc agttccaggc gcgccagcgg
     2221 catgcccgca cacatgtgcg gtcccatgcc gaacgccaga tgctggtcga tgttgggtcg
     2281 attcatgtcg aactgatccg catgttcgaa aacggcctca tcccggttgg ctgatgcata
     2341 cagtagcgca atcggctcac cttcccgaat cgcgcggccg ttgatttcga catcgcggtt
     2401 ggcggtacgg gcaaatccgc ggtagggcgt gtacagacgc aggaactctt cgatcgcgcg
     2461 cggcatcgcc gacaggtcgg cgcgcagccg ctgctgcagc ggcggatcgc gcgccaggtg
     2521 caccgcgatg ctgcccacga cgatcgtcgg ggcgatgatg ccaacgacca gcacctgacg
     2581 gatcgtgccc aggatcatct cgtcagggaa gggtgcgccg tcgatgcgcg cggccagcag
     2641 cgcggcggtg ggatccgtcg ccggatcgcg tggctcctgc cgccgactgg cgatcatggc
     2701 gcgtgccatg tcatagagct tgagactggt ttccctgacc atttcaccgt tggccgactg
     2761 cacggcgatg ttgtaggcgc ggccgacctg ctgcaggtcg ggcaccatcg attcgggcac
     2821 gtccatccac tcggcaaaga cacgtatggt caggtggctc gagtagtcgg aacacagatc
     2881 accgccaccg cgcgcaatca tggcttgcag cagcgatgcg gccagcgctc gaatcttggg
     2941 ttccagccgc gcgatgcgct cgggtttgaa cagcggattc agggcgcgcc ggtagggcgt
     3001 gtgctccggc gggtccaggt gcagcggcgg gcgccggccg gtaaaggcca gcctcggaac
     3061 cacattctgt accgacgtga tgaaaaccga ggactgttgc agtgcttgct gcacctcggc
     3121 gtagcgggtg agggcccaga aaccacccca cgcgtcggag tgtgccaccg ggcagcgcgc
     3181 gcgcagttcg cggtagacct cgtgcggact gtcgaagctc tcggcaatga gcggatcgaa
     3241 gtcctgcggc tcccgggcag ctgtcatgcg gtcaatgtag catcgttcca ctatctatga
     3301 aagcgcttac aaaagcgtac actctgcagc atggaaagcc tccgtcattg cgccaggact
     3361 gggccgcgct ttggcatcgt actcggcctg ctggcgctga acccggtcgc ggggctggcc
     3421 cagaatgact cgtacgatgg cgccaactgg tggcagagcg gaagcggtca gacgctgggc
     3481 gccagcgcgc agtatgacga tgcgctcgga agagtcggct tgctcaatgc cgcgggcgcc
     3541 atcgatacca ggggccaccc gttctttgag ccgatcgggg gcaatggccg tgcgtgtgtg
     3601 acctgtcatc agcccgccaa cgcgatgagc agctcggcgg cggcgatccg cgcccgatgg
     3661 caggctacca acggccgcga cccattgttc gccgccgtgg atggctcgaa ttgtccgagc
     3721 gcgccgcagg aactcgaaag ctcgcattca ctgctgctgc gccatggcct gtttcgcatc
     3781 tatctgccgt ggccgccgaa agcggcggac ggctcgacca tcacaccgga gttcgacctg
     3841 gaactggtga gtgatccgag cggctgcaac tcggatcctg tctacgggct gcacagtgcg
     3901 catccgcagg tgtcggtgtt ccgccgcacg cgcgtggtcg cgaacatgaa gtatgtgacc
     3961 ggtggcggcg gtgcagcgtt caacatcaag gatggctcgc cgatggcgcg cgatccggag
     4021 accggccgct tcgtcaacat gaacctgtta tccgacgcgc gcgctccgac gctgaaaacc
     4081 caggcgatcg atgcggcgat gacccatcta caggcctccg cggcgccgtc cgcagcgcaa
     4141 ctgctgagca tcgtgcagtt cgaggatcag gtgtacgtgg ccgcagagtt ctccagcaag
     4201 ggcggcagtc ttaccgagcc cggcgcgccg caggatctgg ggccggccgc gatgttcaag
     4261 gaacagcccg gcctgggaga caatttctac cagccggtgt tcggctattt cgatcagtgg
     4321 gcggggttga gcggcgccga tccgcagtcg cagtttcgcg cctcgatcgc acgcggcatg
     4381 caggtgttct tcacgcgccc gttcttcatt cgcgacgtca cgcatctgaa tacggtgggc
     4441 ctcggcaatc cgatccggcg tagctgcgcg acctgtcaca acgcgcggat gaccgggatg
     4501 gactttgcgc ccggctggat ggatctcgga accggcaaca agccgtgggc cgatgatctg
     4561 cccatgacct cggagttgcc gttgttcaaa ctgacctgca aggcctcggc cccgtcgcat
     4621 ccgtttctgg ggccggtcat ctatacgcat gatccgggca gggcgctgat ctccggacgc
     4681 tgcatcgaca ttggagccat cacgatgcag cagtttcgcg gtctggccgc ccgcgcgccg
     4741 tatttttcga acggctcggc cgatgatctg cgggcactgg tggagtatta cgacaagcga
     4801 tttgaagccc actacaccga gcaggaaaag caggatctca tcaacttcct gagcgtgcta
     4861 tgagcagcca gctgtcggat cgcgcatttc ggctcgccgt cgccacggcg ctcgctctgc
     4921 tcgcgtcctg tctggccgcg gcggcggatc cggggcggga gatcgtattc gtcggctgcc
     4981 cgattctgcg caataccgag ctgccgtgct ggctgggaga gtcgggcggg gagctctatt
     5041 atcttggcgc gcagggggat ctgacctcgc agttctatcc gccgcagttt gatcatcgca
     5101 tgctggtgga aggcatcgtc agtgacgagg ctcgcatctg cggcgggcgg gtgttgaagc
     5161 cggtgaaagt ctcggtgctg ccgcag
//
LOCUS       DATMRM010000407         3448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_217198, whole genome shotgun sequence.
ACCESSION   DATMRM010000407 DATMRM010000000
VERSION     DATMRM010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217198"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="VNZ06_12365"
     CDS             complement(<1..1143)
                     /locus_tag="VNZ06_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWW21595.1"
                     /translation="MTAKSQRDAYDVIVVGSGAAGGQSAYTLCMEGAKVLMLEAGRRY
                     SPETETPMFQTPDLAPLAAVGTPQKPFGFYDATLDGGWEVPGEPYVRASEEAAGRFEW
                     WRARMLGGRTNHWGRISLRYGPYDFKPHGRDGLGFDWPIGYQDIAPYYDKVELLIGVY
                     GSNEGLENTPDSSPGCLLPPPKPLVSDLLMAQRARRLGIPAIPGHRAVLTRPLDFRSS
                     PARLHPGNVTAQKILADDMRHRAACIWATPCGRGCSIRANYQSTTVHLPPALASGNLD
                     ITTDAMVYEVTLGRDGRANGVSFVDRTTGRQQHATARVVILAASACESVRILLNSRSA
                     RFPNGLANSSGRVGRYLMDTVGSTVSGQIPLLESLPPLNEDAAGGDQ"
     gene            complement(1173..2384)
                     /locus_tag="VNZ06_12370"
     CDS             complement(1173..2384)
                     /locus_tag="VNZ06_12370"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HWW21596.1"
                     /translation="MKLSILTAALQELTPRDRRDADPDVAIEEWLEFARQIGCPNIEL
                     SAALHPTESDVPAAALLDPVANTLDLRQPFNAERATRVRRTMQATGVGLSDLAYFDNM
                     LAADESVRRKKHELMRRVFDAAVLLGTDAVAGFVGRNPLLGMDANLVMFEQVFVPLLK
                     EAQARGLTFRVEQCPMPGWNITDAWHNNIAYAPGPWIALHRICERYGVGDQFRIHYDP
                     SHAILMGQDSRSVFQYLKDTGYNFLITGFHVKGQVIDSRGISAWGYGGQTMQRGDWHG
                     DRPSADPAQQRNAWKKQTVFCEHELPGTARHDPLAYLQNRSVDWLDHQLAARELLSIA
                     PEKTFLVVEHEYPKARVQDKARLAPILAGSLAFTRAIDEAAAAMYALQHEVLAAQGIS
                     VQGIGREAYRS"
     gene            complement(2496..>3448)
                     /locus_tag="VNZ06_12375"
     CDS             complement(2496..>3448)
                     /locus_tag="VNZ06_12375"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW21597.1"
                     /translation="KAFYPCLLAYMILYMPTLALVNTISFRQMDEPARHFSRVRMWGT
                     IGWIVAGLGVSYLFAWDSPAGMAQGSLGNTFLMCSIASIALGIYSFTLPRTPPLPKPE
                     EQARLGHVLGLDALVLLRHKNFVVFFVASILICIPLAFYYQNANQFLTEIHVANATGK
                     QTIGQMSEVLFMLLIPLFLNTFGMKTTLLLGMLAWVSRYLLFAFGNAHGLAFMLILGI
                     ALHGACYDFFFVSGQIYTDSKAGARHKAAAQGLITLATYGVGMLVGFWAAGLIDDSYS
                     AGGAHDWKSIWLYPAAFAAVVLVLFAATFRNERVAYATAH"
BASE COUNT          715 a         1079 c         1033 g          621 t
ORIGIN      
        1 ctggtcgcca cctgcagcgt cctcattgag cggcggcaga ctctcgagca ggggaatctg
       61 cccgctcacg gtgctgccaa ccgtatccat cagatatcgc ccgaccctgc cgctggagtt
      121 ggccagtccg ttcggaaacc gcgcgctcct ggaattgagc agaatgcgga ccgattcaca
      181 cgcactcgcc gccaggatca ccacccgtgc ggtggcgtgc tgttgcctcc cggtcgtgcg
      241 gtcaacgaag ctcacgccgt tggcccggcc atcgcgcccg agcgtgacct cgtaaaccat
      301 cgcatcggtc gtgatatcga gattcccgct ggccagcgcg ggcggcaaat gcaccgtggt
      361 cgactgatag ttcgcgcgga tggagcagcc gcgcccgcac ggtgttgccc agatacaggc
      421 cgcgcggtga cgcatgtcgt cagcgaggat cttttgcgcc gtaacattgc ccgggtgcaa
      481 gcgtgccggg gagcttctga aatccagcgg ccgggtaaga accgctcgat ggcccggtat
      541 cgcaggtatg ccgaggcgcc tggctcgctg ggccatcaac aggtcactca ccagcggctt
      601 gggcggcggc agcaggcagc ccggtgagga atccggcgtg ttctccaggc cctcgttgct
      661 accgtatacg ccaatcagca actcgacctt gtcgtagtac ggcgcgatgt cctggtagcc
      721 gatgggccaa tcgaacccca gcccatcccg tccatgcggc ttgaagtcgt acggaccgta
      781 gcgcagcgaa attcgccccc agtgattggt gcgaccaccc agcatgcgcg cccgccacca
      841 ttcgaagcga cccgcagcct cttcgctcgc gcgcacataa ggctccccgg gtacctccca
      901 gcccccgtcc aacgtcgcgt cgtagaagcc aaagggcttt tgcggtgtac cgacagccgc
      961 aagcggcgcc aaatccggcg tctggaacat gggtgtctcg gtttcggggg aatacctgcg
     1021 cccggcctcg agcatgagta ccttcgcacc ctccatacac agcgtatagg ctgactgtcc
     1081 gccagcggcc ccggagccaa ccacgatgac atcgtaggcg tccctttggg atttcgccgt
     1141 cacgaagggc ataccgatac cactcgatca gttcaggatc gataggcttc gcggccgatg
     1201 ccctgcacgg atatcccctg ggcggcgagc acctcatgct ggagggcgta catcgccgcg
     1261 gcggcctcgt caatcgcgcg cgtaaaggcg agggacccgg ccagaatcgg cgccagccgt
     1321 gccttgtcct gtacgcgagc cttgggatat tcgtgttcca cgaccaggaa ggtcttttcc
     1381 ggcgcgatgc tgagcagctc ccgtgcggcc agctgatggt ccagccagtc taccgatcga
     1441 ttttgcagat acgcgagcgg atcatggcgc gcagtgccgg gcagctcgtg ctcgcaaaag
     1501 acagtttgct ttttccaggc attgcgctgt tgagccggat cggcgctcgg ccggtccccg
     1561 tgccagtcgc cgcgttgcat cgtctggcca ccgtagcccc atgctgagat accgcgcgaa
     1621 tcgatgacct ggcccttcac atgaaagccg gtgatcagaa agttataccc tgtgtccttc
     1681 agatactgaa acacggagcg cgagtcctgc cccatcagga ttgcatgcga gggatcatag
     1741 tggatgcgga attgatcgcc gacgccgtac cgttcacaga tccggtgcaa tgcaatccac
     1801 ggacccggag cataggcaat gttgttatgc cacgcatcgg taatattcca ccctggcatg
     1861 ggacactgct cgacccgaaa ggtcagcccg cgggcttggg cctccttcag cagcggcacg
     1921 aacacctgtt caaacatcac cagattcgcg tccattccca acagcggatt gcggccgaca
     1981 aagcccgcga ccgcatcggt cccgagcagt actgcggcgt caaagacgcg ccgcatcagt
     2041 tcatgcttct ttctccgcac tgattcatct gcggcgagca tgttgtcgaa gtaggcgaga
     2101 tccgacaggc ctacgcccgt ggcttgcatt gtcctgcgga cgcgtgtggc gcgttcagca
     2161 ttgaagggct ggcgcagatc cagggtgttg gccacaggat caagaagtgc cgccgccggc
     2221 acatcgcttt ccgtcgggtg cagggccgca gagagttcaa tatttgggca accgatctgc
     2281 ctggcaaact ccaaccactc ctcgatagcc acatccggat cggcgtcgcg ccggtcgcgg
     2341 ggagtcaatt cctgtaacgc cgccgtaagt atgctgagct tcatcgctct cggccggaac
     2401 tctctgcccg gtgatgtcgg tacaggtgaa ctggcgtgtt cgctcaagat ggcgtgctcc
     2461 gaagtaacca agcgctcaat ggcgcccgca aaacattagt gcgcagtggc atacgcgacc
     2521 ctctcattgc gaaacgtcgc ggcgaacagc acgagcacca cggctgcaaa ggcggccggg
     2581 tacagccaga tggacttcca gtcatgcgcg ccgccggcgg agtagctatc gtcaatgagg
     2641 ccggcagccc agaacccgac cagcatgccg acaccgtagg tcgccaaggt tatcaggccc
     2701 tgggctgcag ctttatgccg tgcgcccgcc ttggagtcgg tgtagatctg gccggagaca
     2761 aaaaagaagt catagcacgc gccatgcagc gcaataccca ggatcagcat gaaggccagt
     2821 ccatgcgcat tgccaaaagc gaacagcaga taccgactga cccaggccag catacccagg
     2881 agcagagtgg ttttcatgcc gaaggtattc aggaacagcg gaatcaacaa catgaacagc
     2941 acttccgaca tctgaccgat ggtctgcttt ccggtcgcat tggcgacatg aatctccgtc
     3001 agaaactgat tggcgttctg gtaatagaac gcgaggggaa tacagatcag gatcgacgct
     3061 acaaagaaca ccacaaagtt cttgtgccgc aataggacca gtgcatcaag acccaggacg
     3121 tgcccgaggc gcgcctgttc ctcgggctta ggcaggggcg gggtgcgcgg caacgtgaag
     3181 ctgtagatgc cgagcgcaat cgaggcaatc gagcacatga gaaaggtatt gccaagcgag
     3241 ccttgtgcca tgcccgcagg tgaatcccag gcgaacagat aactgacacc caagccggcg
     3301 acgatccagc cgatcgtgcc ccacatgcga acacgggaga agtgtctggc gggctcatcc
     3361 atctgccgaa acgagatggt gttgaccagc gcgagcgtcg gcatatacag aatcatgtac
     3421 gcgagcaggc agggatagaa ggctttga
//
LOCUS       DATMRM010000408         4338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1171861, whole genome shotgun sequence.
ACCESSION   DATMRM010000408 DATMRM010000000
VERSION     DATMRM010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171861"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..668)
                     /gene="pth"
                     /locus_tag="VNZ06_12380"
     CDS             complement(66..668)
                     /gene="pth"
                     /locus_tag="VNZ06_12380"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HWW21598.1"
                     /translation="MAGLSLKLIVGLGNPGGEYARARHNAGWWWVDELARRYRGSWRA
                     QPREHTELARVALEGSELWLLKPTSHMNNSGTPTAAVARFHRISPSEILVVHDEIDLP
                     PGIARLKSGGGHGGHNGVRDVMAHLGADFWRLRLGVGHPGSKDLVIGSVLDRPTAAEH
                     QAIWDAISRSLDIVPDLLRSGAQKAMHTLHSANPKQSADS"
     gene            complement(827..1579)
                     /locus_tag="VNZ06_12385"
     CDS             complement(827..1579)
                     /locus_tag="VNZ06_12385"
                     /inference="COORDINATES: protein motif:HMM:NF004128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HWW21599.1"
                     /translation="MRITYQVGADSRHDQGKGASRRLRRAGKVPAVLYGGHGQPLSIV
                     LDHQQLLTLIDKEKFYSSIISINVDKQGQPAIVRDVQMHPARNAVVHVDLQRVIENEK
                     LKIHLPIHFKGEATAPGVKLQGGIVSHLIQDIEVSCLPKDLPEFLELDLSGMNLNDTK
                     HLSDIPLPTGVTIPALAHQNPPVVSVHSPRVAEPEPEAAAAAATEGAAAAAPAAGAEA
                     AAAPAAGAAGKKEEPKKEDAKKEAAPKKEAKK"
     gene            complement(1648..2604)
                     /locus_tag="VNZ06_12390"
     CDS             complement(1648..2604)
                     /locus_tag="VNZ06_12390"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HWW21600.1"
                     /translation="MTESSELALFTGNANPALAHDIARHLRLPVGRASVGRFSDGEVN
                     IEIMENVRGRDVFIVQPTSPPANDHLMELLVMVDACRRASASHITAVVPYFGYARQDR
                     RPRATRAAITAKLVADMIGQVGVDRLLTLDLHSDQIQGFFAIPVDNVYASPVLLGDVW
                     KQGYANLIVVSPDVGGVVRARAIAKRLDDADLAIIDKRRPRPNESKVMNIIGEVEGRT
                     CVLVDDMVDTAGTLCLAAQALKDEGALKVVAYATHAVLSGGAVERVSKSAIDELVVTD
                     TIPLSQNARDCGRIRQLSVSSLLAETIRRIRDDDSVSSLYID"
     gene            complement(2630..2704)
                     /locus_tag="VNZ06_12395"
     tRNA            complement(2630..2704)
                     /locus_tag="VNZ06_12395"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2670..2672),aa:Gln,seq:ttg)
     gene            complement(2716..3582)
                     /gene="ispE"
                     /locus_tag="VNZ06_12400"
     CDS             complement(2716..3582)
                     /gene="ispE"
                     /locus_tag="VNZ06_12400"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HWW21601.1"
                     /translation="MTELSWWPAPAKLNLFLHVVGRRADGYHDLQTLFQLIDRSDQIG
                     IAVREDARIIRTRGLAAVAPEQDLTVRAALALQQHTGCRRGAEIQVRKHIPVGGGLGG
                     GSTDAATVLLVLNHLWGTQLSLPELAKLGLGLGADVPVFIHGTSAWGEGRGERLEPMH
                     LPERWFLVLHPGVSVSTGEMFQAPELTRNSPLITIRAFSEGGTANAFEPVACARHPEV
                     ALALDWLRSQIDAHNAPIKARMTGTGACLFAGFEREVDAERIAARVPDRWNSFVARAL
                     NRSPLHARLGWD"
     gene            complement(3575..4321)
                     /gene="lolB"
                     /locus_tag="VNZ06_12405"
     CDS             complement(3575..4321)
                     /gene="lolB"
                     /locus_tag="VNZ06_12405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HWW21602.1"
                     /translation="MESGVDGRSGQRSGEGRAAALWCADAGEQRQRHLNLSAGRRSAA
                     GGVAVSILIAAIVLPGCALMSKAPQNTLELVPWAERLTALQALYHFEFQGRVAITNGS
                     DGFSAGLRWQQNDDQALVDLSAPLGFGAAHIQQTDNILRVTTSKGVTLESDAATDELR
                     SLLGFDAPLRSLRYWIVGASDPQSPSGVSLDSLQRLTRLDQDGWRIDYGDYTAVKNRA
                     WLPLSLTITHETLKLRIVIHDWQLYSLLGD"
BASE COUNT          810 a         1438 c         1260 g          830 t
ORIGIN      
        1 aagagcgtgg acttgccgac attgggcagg ccaacaattc cgcattgaat gggcattcga
       61 acctctcaac tatccgcgga ctgcttagga ttcgcgctgt gcagcgtatg catcgccttc
      121 tgcgcgccgg accgcagtaa atcaggaacg atgtcgagcg aacgcgatat cgcgtcccaa
      181 atcgcctgat gttcagccgc ggtcggccga tccagtaccg agccgatcac cagatccttc
      241 gatccggggt gaccgacccc gagccgcagg cgccagaaat ctgccccgag atgcgccatc
      301 acatcgcgca cgccattgtg tccgccgtga ccgccgccgc ttttgagtcg ggcgattccc
      361 ggcggcaaat cgatctcatc gtgcacgacc aggatttcag acggtgaaat ccgatggaac
      421 ctggcgactg cggcggttgg tgtgccgctg ttgttcatgt gcgaggtcgg cttcagcagc
      481 cagagctcac tgccttcgag cgcaacgcgt gccagctccg tgtgctcgcg cggctgcgct
      541 cgccagctgc cgcgatagcg cctggccaac tcatcgaccc accaccagcc ggcgttgtga
      601 cgcgcgcgcg cgtattcccc gccgggattg cccagaccga caatgagctt gagtgaaagt
      661 ccagccatag aaggccacgc ccagtcaatc gaaaaccctg aacaccgcgc tgtgctggga
      721 gccgactgga tgcgccgacc tatacacgca tttcttctag cagaaatgcg tgtgcagctc
      781 agggcatccg attgactgcc aatgcagcgc tgttcgtgtt tccgatttac ttctttgctt
      841 ccttcttggg ggccgcctct ttcttggcgt cctccttctt cggctcttcc ttcttgccag
      901 cagcaccggc ggccggtgcc gccgcagctt ccgctcccgc agccggggcc gcagccgcgg
      961 caccttcggt cgccgccgca gcagccgctt ccggctccgg ctcggcgact cgcggactat
     1021 gcaccgacac gaccggcgga ttctggtgcg ccaatgccgg aatcgtgacg ccggtcggca
     1081 gcggaatgtc cgacagatgc ttggtgtcgt tcaggttcat gcccgacaga tccagctcga
     1141 ggaattccgg gagatccttc ggcagacagc tgacttcgat gtcctgaatc agatgcgaca
     1201 cgatgccgcc ctgcagcttc acgccgggcg ccgtggcctc acccttgaag tgaatcggca
     1261 gatgaatctt cagtttctca ttctcgatca cgcgctgcag atcaacatgc accacggcgt
     1321 tgcgcgccgg atgcatctgc acgtcgcgca cgatcgcagg ctgaccctgc ttgtccacgt
     1381 tgatgctgat gatcgaggaa tagaattttt ccttgtcgat cagcgtcagc aactgctgat
     1441 ggtcgagaac gatgctcagc ggctggccgt ggccaccgta gagcacggcg gggactttgc
     1501 cggcacgacg caggcggcgg ctcgcacctt tgccctgatc gtgacgcgag tccgcgccaa
     1561 cttgataggt aatacgcatg ttcaaactcc tgactattga cgaacacgaa cgcccaccgc
     1621 gaccagtgag cgttcaccag aaaaaagcta gtcgatatac agcgaactga ccgaatcgtc
     1681 gtcgcggata cgacggatcg tttcggccag cagcgaagac accgacaact gacggatgcg
     1741 tccacaatca cgcgcgttct gcgacagcgg aatcgtgtcg gtcaccacca gttcatcgat
     1801 cgccgatttc gaaacccgtt ccacggcgcc gccggacagc accgcgtgcg tggcataggc
     1861 cacgactttc agcgctcctt cgtccttcag cgcctgcgcc gccaggcaca acgtgccggc
     1921 ggtatccacc atgtcgtcga ccagcacgca ggtgcgtcct tcgacctcgc cgatgatgtt
     1981 catgactttc gattcgttcg gtcgcggtcg ccgcttgtcg atgattgcca gatccgcatc
     2041 gtccagacgc ttggcgatgg cacgcgcgcg cactacacca ccgacgtccg gcgacacgac
     2101 gatcagattc gcgtagccct gcttccacac atcgcccagt agcaccgggg acgcgtagac
     2161 gttgtcgacc ggtatcgcaa agaatccctg gatctggtcc gaatgcagat cgagcgtcaa
     2221 caaccggtcg actcccacct gcccgatcat gtccgcgacc agcttggcgg tgatcgccgc
     2281 gcgtgtggca cgcggccgcc ggtcctggcg tgcataacca aaataaggca ccaccgccgt
     2341 gatgtgcgat gccgaagcac gccggcaggc gtccaccatc accagcagtt ccatcagatg
     2401 atcgttggcg ggcggactgg tcggctgcac gatgaataca tcgcgcccgc gcacgttctc
     2461 catgatctcg atgttgactt caccatcact gaaacgaccg accgaggccc gaccgaccgg
     2521 tagccgcaag tgtcgtgcga tgtcatgcgc cagcgcaggg ttcgcattcc cggtaaacag
     2581 cgccagctct gacgattccg tcatcggtcc ctatcgttcc tgttgttcgt ggctggggtg
     2641 gaaggattcg aaccttcgta tgacgggatc aaaacccgtt gccttaccac ttggcgacac
     2701 cccaacaatt tccaatcagt cccatccaag tctcgcgtgc aatggcgagc gattcagcgc
     2761 gcgcgctacg aaactgttcc accggtctgg aacccgtgcc gcgattcgct ccgcatccac
     2821 ctcgcgttca aaaccggcaa agaggcacgc accggtgccc gtcatgcgag ccttgatcgg
     2881 tgcgttgtgc gcatcgattt gcgaccgcag ccaatccagc gccagcgcga cctcggggtg
     2941 gcgcgcgcac gcgaccggct caaacgcatt ggcggtaccg ccttccgaaa aggcgcgtat
     3001 tgtgatgagg ggggaatttc gtgtcaattc aggggcctgg aacatctcac cggtactgac
     3061 gctaaccccc gggtgcagca ccagaaacca gcgttccggc aagtgcattg gctcgagccg
     3121 ctccccgcgc ccttcccccc aggcgctggt tccgtgaatg aacaccggta cgtccgcccc
     3181 aagcccaagt cctagtttcg ccagctccgg caggctcaat tgcgtccccc acagatggtt
     3241 gagtaccagc aggacggtgg cggcgtcggt acttcccccg cccagccctc cgccgaccgg
     3301 aatatgtttc ctgacctgga tctcggcacc ccgtcgacag ccagtgtgct gctgcagggc
     3361 cagcgctgcc cggactgtca aatcctgttc gggggcgacc gccgccagtc cacgcgttcg
     3421 gatgatccgt gcatcctccc gcaccgcaat gccaatctga tcgctacggt cgatgagctg
     3481 aaagagcgtc tgcagatcgt ggtagccatc ggcgcgtcgg ccaaccacgt gcaggaacag
     3541 attcagtttg gccggggcgg gccaccacga gagctcagtc accgagaaga ctgtacagct
     3601 gccaatcgtg gattacgatc ctgagtttca gtgtttcgtg tgtgatcgtc aggctcagcg
     3661 gcagccacgc cctgttcttg acggccgtgt agtcgccgta gtcgattcgc cagccgtcct
     3721 gatccagacg ggtcaatcgc tgcagcgagt cgagcgagac cccggaagga ctctgcggat
     3781 cgctggcacc cacgatccag tagcgcagac tgcgaagtgg cgcgtcgaag cccagcaggc
     3841 tacgcagttc atcggtcgcg gcatcgctct cgagcgtcac acccttgctg gtggtgaccc
     3901 gcagaatgtt gtcggtctgt tggatgtgcg ccgcgccaaa gcccagcgga gccgacagat
     3961 ccaccagcgc ctgatcgtca ttctgctgcc agcgcagtcc ggcgctgaac ccgtcggagc
     4021 cgttggtgat tgcgacccgt ccctgaaact cgaagtgata cagtgcctgc agagcggtca
     4081 gacgctcggc ccatggcacg agttcgaggg tgttctgcgg cgccttcgac atcaaggcgc
     4141 agcccggcag cactatggct gcgatgagaa tcgataccgc aaccccgccc gcggcggagc
     4201 gccggccggc gctcagattg aggtgccttt gccgttgctc tccagcgtcg gcacaccaaa
     4261 gcgctgctgc gcggccttca cctgagcgtt gtccggatcg accatcaacg cccgattcca
     4321 ggtttcgcgt gccttggc
//
LOCUS       DATMRM010000409         3372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3629739, whole genome shotgun sequence.
ACCESSION   DATMRM010000409 DATMRM010000000
VERSION     DATMRM010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3629739"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VNZ06_12410"
     CDS             <1..712
                     /locus_tag="VNZ06_12410"
                     /inference="COORDINATES: protein motif:HMM:NF013118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HWW21603.1"
                     /translation="QPGGVLTRAGHTEAGCDLARLAGFQSAAVIVEIMNDDGTMARRP
                     QLEAFAASHQLKIGTIADLIRHRLQTERSVERIAEQTVLTEHGEFRLVCYEDHVNHDV
                     HLALVHGDLRTDTPLVRVHLTDTLRDLIGVRQGSKAWTLNAALQRVARSPCGVVVLLR
                     QAEQPRELVDAVRSVGMPTSEPSPQLSGPVLRTYGIGAQILQDLGVRRMRVLSAPKHM
                     YSISAFGLEIVDYVGESG"
     gene            709..1188
                     /gene="ribH"
                     /locus_tag="VNZ06_12415"
     CDS             709..1188
                     /gene="ribH"
                     /locus_tag="VNZ06_12415"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HWW21604.1"
                     /translation="MKLGTAAQQSADFSASDCKFAIVAARYNEDIVSALLQGAQAAWR
                     AHGGAETALRIERVPGAFELPMAARAFALEGAVDAVIALGCVIRGDTAHFDFVAGECA
                     HGLQRVMLDTGVPVSFGVLTVENHPQALERALPDKLDKGGEAVTTALAMVHLLRRLK"
     gene            1185..1622
                     /gene="nusB"
                     /locus_tag="VNZ06_12420"
     CDS             1185..1622
                     /gene="nusB"
                     /locus_tag="VNZ06_12420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWW21605.1"
                     /translation="MSGRPERSSGARTVARKLALQALYRWQLNSCEWQDLVQEFSSDP
                     DMPRADAEYFQALISGIVRSRDELDGALKPLLDREPTQLDPIEHAVLLIGGYELQHRP
                     EVPFRVVINEAVSLARRFGATDGHKYINGVLDRAAHLWRSHEH"
     gene            1644..2597
                     /gene="thiL"
                     /locus_tag="VNZ06_12425"
     CDS             1644..2597
                     /gene="thiL"
                     /locus_tag="VNZ06_12425"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HWW21606.1"
                     /translation="MDETHLIDRYFRDLGARRGDVALGVGDDAALLRVPTGAELVLTT
                     DALIEGAHFLPGAAAHSLGHRAMAINLSDIAAMGATPAWALLSLNLPAADEAWLREFA
                     DGFGALAARHNVALVGGNLGRGPLSVTVQLAGLVPMGQALRRDRACPGDCLYVSGCIG
                     DAAQGLLQLGSTGGDADAQYLRSRLEYPTPRVALGEALRGVASACIDISDGLYVDAQR
                     LLRASGCGAALQIEALPLSDALRRAAGSDAWQRGLRGGEDYELCFSAAPQFSDRVAEL
                     AQTTGQVVSCIGRLCRETGITLRSGNSVIQFSTSGWDHFRD"
     gene            2740..>3372
                     /locus_tag="VNZ06_12430"
     CDS             2740..>3372
                     /locus_tag="VNZ06_12430"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWW21607.1"
                     /translation="MAASAPITATVRTIPERIGKYLIINEVGRGSTGNVFLSHDPYYG
                     RDVAIKVYNIDSGGDDERARIARKMFLSEAHMVGMLHHPNILPIYDAGEESGHCYIVT
                     EHVHGARTLSTYCRADNLLRLDDVVELMFKCAKALHYAHSRGVIHRDIKPSNIMLTND
                     SDVRIIDFGIALVAGSEISRIEGIAGSPSYMSPEQVQSLELTNRSDLYSLG"
BASE COUNT          563 a         1050 c         1137 g          622 t
ORIGIN      
        1 ccagcccggt ggggtgctca cgcgggccgg tcacaccgag gcgggctgcg atctggcgcg
       61 tctggccggc tttcagtccg ccgccgtgat cgtcgagatc atgaacgatg acggcacgat
      121 ggcgcggcgt ccgcagctgg aggcatttgc cgccagccac cagctcaaga tcggcacgat
      181 cgccgacctg atccggcatc gcctgcagac cgagcgttcg gtcgagcgca tcgcggagca
      241 gaccgtgctg accgagcacg gtgagtttcg tctggtctgt tacgaggacc acgtgaatca
      301 cgatgtgcat ctggccctgg tgcatggcga tctgcggacc gatacgccgt tggtgcgcgt
      361 gcatctgacc gacacgctgc gcgatctgat cggggtgcgt cagggatcca aggcctggac
      421 gctcaacgca gcgctgcagc gcgtcgctcg ctcaccctgc ggagtcgtgg tgttgctgcg
      481 ccaggccgag cagccacggg aactggtgga tgcggtgcgc tcggtgggca tgccgaccag
      541 tgagccgagt ccacagctga gcggtccggt gcttcgcacc tatggtatcg gtgcgcagat
      601 cctgcaggac ctgggtgtgc gtcgcatgcg cgtgctgtcg gcgcccaaac acatgtacag
      661 catttccgcc ttcggcctcg aaatcgtcga ctacgttggc gagagcggtt gaagctggga
      721 acggcagccc aacaatctgc cgacttttcg gcgagcgact gcaaattcgc gatcgtcgct
      781 gcccgctata acgaggacat cgtgtcggcg ttgctgcagg gagctcaggc cgcgtggcgg
      841 gcgcatggtg gtgccgagac ggccctgcgg atcgaacgcg tgcccggcgc tttcgaactg
      901 ccaatggcag cgcgtgcttt cgcactggag ggcgcagtgg atgcggtcat cgcactcggc
      961 tgcgtgattc gcggcgatac ggcgcacttc gattttgtcg cgggcgaatg cgcccacggg
     1021 ctgcaacggg tgatgctcga caccggtgtg ccggtgagct tcggtgtgct gacggttgag
     1081 aatcacccgc aggcgctcga gcgcgcgctg ccggacaaac tcgacaaagg cggcgaggcg
     1141 gtcaccactg cgttggcgat ggtgcacctg ctgcgccggt taaagtgagc gggcgtcccg
     1201 agcgcagctc gggcgcgcgc acggtcgcac gcaagctcgc actgcaggcc ctctatcgat
     1261 ggcagctcaa cagctgcgag tggcaggatc tggtccagga attctcctcc gatccggaca
     1321 tgccgcgcgc ggacgccgaa tactttcagg cgctgatttc gggcatcgtg cgttcgcgcg
     1381 acgaactgga cggcgcattg aagccgctgc tcgatcgtga gccgacgcag ctcgatccca
     1441 ttgagcacgc agtgctgctg atcggtggct acgaactgca gcatcgtcct gaggttccgt
     1501 tccgcgtcgt gatcaacgaa gcggtcagcc tggcgcggcg ctttggcgct accgacggtc
     1561 acaagtacat caacggtgtg ctggaccgtg cggcgcacct gtggcgttcg cacgagcact
     1621 gacgccaggc gcggccgcgc acgatggacg agacgcacct gatcgatcgg tactttcgcg
     1681 atctcggcgc gcgtcggggc gacgtggcct tgggggtagg cgatgacgcg gcgctgctgc
     1741 gcgtacccac gggtgcggaa ctggtgctca cgaccgatgc gctgatcgaa ggtgcgcatt
     1801 tccttcccgg agccgcggcc cattcgctcg ggcatcgcgc gatggcgatc aacttgagcg
     1861 acatcgccgc gatgggcgca acccccgcct gggcactgct gtcgctgaac ctgcccgccg
     1921 cggatgaagc ctggttgcgc gaatttgcgg acggcttcgg cgcactggcc gcgcgacata
     1981 atgtcgccct ggtcggcggc aatctgggtc gcggaccact gtcggtgacg gttcagctcg
     2041 caggtctggt gcccatgggg caggccctgc ggcgcgatcg ggcctgtccg ggggactgtc
     2101 tgtacgtcag cggctgcatc ggtgatgccg cgcaggggtt gctgcagctg ggcagcaccg
     2161 gcggcgatgc cgatgcgcaa tacctgcgca gtcggctcga gtatccgaca cccagagtgg
     2221 cgctgggcga agcgctgcgc ggtgtggcca gcgcctgtat cgatatttca gacggactgt
     2281 acgtggacgc gcagcgcttg ctgcgggcgt cgggctgcgg cgcagcgttg cagatcgagg
     2341 ctctgcccct gtcggatgcg ttgcgacgcg ccgccggcag tgacgcctgg cagagggggc
     2401 tgcgtggcgg tgaggactac gaactgtgtt tcagcgctgc gccgcaattc agcgaccgcg
     2461 ttgctgaact ggcgcaaacc accgggcagg tggtgagctg tatcgggcgt ttgtgtcggg
     2521 aaaccggcat cactttgcga tcgggcaatt cggtgatcca gttctcgact tcggggtggg
     2581 atcactttcg cgactgaaca atgtgtggcg gtcttttgac gctccttgcg gcgcatattc
     2641 ggcgcagttt gggccaccaa cgatccgcga tccgcgtcac agccatggcg ggcaggcgcc
     2701 gcttattctg gcgtccgcac acaaagaccc gcgacaagaa tggcagcgtc tgcaccgatc
     2761 acggccaccg tccgcacgat tcccgagcgc atcggcaagt atctcatcat caatgaggtc
     2821 ggccgcggca gcaccggcaa cgtcttcctg tcgcacgatc cctattacgg gcgcgacgtc
     2881 gcgatcaagg tctacaacat cgattccggc ggtgatgacg agcgcgcgcg catcgcgcgt
     2941 aaaatgtttc tgtcggaagc acacatggtc gggatgctgc atcacccgaa catactgccg
     3001 atctacgacg ccggcgaaga aagcggtcac tgttacatcg tcaccgagca cgtgcacggc
     3061 gcgcgcacgc tgtccacgta ctgccgcgcc gacaacctgc tgcggctcga cgatgtggtc
     3121 gaactgatgt tcaagtgcgc caaggcactg cactacgcac attctcgcgg tgtgattcac
     3181 cgggacatca aaccctcgaa catcatgctc accaacgata gcgatgtgcg catcatcgat
     3241 ttcggtatcg cgctggtcgc aggctcggag atttcccgca tcgagggcat cgccggctcg
     3301 ccgagctaca tgtctcccga gcaggtgcag agtctcgagc tcaccaaccg ctcggatctg
     3361 tactctctcg gt
//
LOCUS       DATMRM010000410         6938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3018237, whole genome shotgun sequence.
ACCESSION   DATMRM010000410 DATMRM010000000
VERSION     DATMRM010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3018237"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..604
                     /locus_tag="VNZ06_12435"
     CDS             224..604
                     /locus_tag="VNZ06_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003199125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HWW21608.1"
                     /translation="MSFLVVDDFSTMRRIIKNLLNDLGYANITEADDGTTALPMLKNG
                     KFDFLITDWNMPGMPGLDLVKHVRADARLGKMPVLMLTAEAKREQIVEAVQAGVNGYV
                     IKPFTAATLKEKIEKILASQTAAA"
     gene            611..1321
                     /locus_tag="VNZ06_12440"
     CDS             611..1321
                     /locus_tag="VNZ06_12440"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HWW21609.1"
                     /translation="MSGRLLDNSEFVSWLGAMSRALAEGNEEALGTALAGFEGVRNAT
                     VTMQVRRVASDLQFALDDFHANAKLVDMAQRRVPDARHRLAHVLRLTDDAAHRTMDLV
                     ERSAPLADHATHEADRLIQLQQQGSTAQQIAPQLTAFVTLVSASMGAVRSNLAEVLIA
                     QGYQDLTGQIIRGVMKLTQELESSLADLLRIAGPANGEEAINATAYGTHGPAVPGVVE
                     DAVGGQQDVDALLSQLGV"
     gene            1321..3417
                     /locus_tag="VNZ06_12445"
     CDS             1321..3417
                     /locus_tag="VNZ06_12445"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HWW21610.1"
                     /translation="MTIDLTQFHDAFYEESSEAIGQMEHALLRLDAGTPDPELINTIF
                     RVAHSIKGGAATFGFAEITSFTHTLETLLDELRGQRLKVTVTLSDLLLRSIDVMREML
                     RAQQAQQPIDQQRVADLQFDLELAVAQKVSAPAAASAPESAAVPGVTSALEVQAPLLP
                     ERHHGTTYQINFRPYLHLLARGNDPLRMFRELRDLGDLQVTVNLAALPPIEELDPENC
                     YLSWQLMLNTDSPRQSVIEVFDWAEGDCELEVITLDAPGSAAAAIAAATAAQSDGPGA
                     AAANGAEALHARAHKANEAVRAVEAQKSGEAGSIRVSVEKIDDLMNSVGELVITQSML
                     GQLGGLLDDALGERLRSGLAQLERNVRELQESVMRVRMVPISFVFSRFPRMVRDLSQR
                     LNKQVELKMTGGETELDKTVLEKIGDPLVHLVRNSVDHGIEMPAQRLAAGKDPCGVVH
                     LHAYHRGSTIVVEVTDDGGGLRRERILEKARERGLIGPTEELTDEAIFNLIFVAGFST
                     QEHANDVSGRGVGMDVVKRNVEELGGSIEVHSAPGGGSRFTITLPLTLAIVDGQTIAV
                     GEETYIVPLTAIIESLQMKTAAVNQVVGHGEVLTFRGEYLPVVRLQKHFNSRADRARE
                     ASEGLIMVVEGDGRRVGLCVDELLGQQQVVVKTLEANYGHVEGIAGATILGDGSVALI
                     LDVANLTRTVTEKLAA"
     gene            3571..3957
                     /locus_tag="VNZ06_12450"
     CDS             3571..3957
                     /locus_tag="VNZ06_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW21611.1"
                     /translation="MAKTILAVDDSASMRQMVGVTLRAAGYEVVEASDGDEALEYARK
                     HRVDLVLADVNMPRMDGITLIAQLRTLPNYHLTPLLLLTTESSPERKMQGKQAGATGW
                     MVKPFHPEQLLATLERVLGNQTAVSA"
     gene            3963..4475
                     /locus_tag="VNZ06_12455"
     CDS             3963..4475
                     /locus_tag="VNZ06_12455"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWW21612.1"
                     /translation="MDNGANKADAKSGHSANVEQFLTFQLVGQRYGVQILQVQEIKGW
                     EKPTRLPHSPAHVQGVINLRGAVVPILDLRKRFGLGEAEYGRTTVVIVVKVETPRGEL
                     TAGMVVDGVCEVCNITAEELRAPPEMAAAIDTDFVRGLAMIDDKMLILLDVTRLVSNT
                     LADTAGTKAA"
     gene            4488..4895
                     /locus_tag="VNZ06_12460"
     CDS             4488..4895
                     /locus_tag="VNZ06_12460"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWW21613.1"
                     /translation="MANSRKSPRRSSAASPDEVNSAAASKPAEAAVIDLPESSAQPVN
                     VLTLGASLSIREVAECAVQLKGLLAAGPLDIDAGKLETIDTAGIQLLLAAAAAAQRRG
                     FKLKLRGAQGLKTGAARSLGLSDHLGELAEILP"
     gene            4892..5470
                     /locus_tag="VNZ06_12465"
     CDS             4892..5470
                     /locus_tag="VNZ06_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21614.1"
                     /translation="MSVAESQALAEQPQDGSATTGTKFAGELRRFLELGAAQLDAAIR
                     ESDARVDKLAGAVTSVATDAREIEMQLPALDSPNSAESERARTRIRQLTDALQSHVQA
                     TITSLQFYDKLIQRLTHVRDGLAIPSDSTMHGVDKSSDWSAMLEQVRSRYSMVEERVL
                     FDFMMRGLSADQMLKALTGLRGTATPGELEVF"
     gene            5481..6353
                     /locus_tag="VNZ06_12470"
     CDS             5481..6353
                     /locus_tag="VNZ06_12470"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HWW21615.1"
                     /translation="MAGDTTAIAGGADGKPSREYEFSDQDFQRVRRMVHQQLGIALSE
                     SKRQLVYGRLSRRLRALHLREFDTYLRRVESGDAEELQHFRNAITTNLTSFFRENHHF
                     EFLARQLMPHLEFQNRSSRRIRIWSAGCSTGEEPYSIAMVLLESAPHLAQNWDVRILA
                     TDIDTNVLEHATRGVYSSDRVEKMDSGRLLRWFARKPGEEQFQVSDAVKQLVTFRALN
                     LISDWPMKGPFDVIFCRNVVIYFDRDTQRQIVGRMEGLQRPGDYLILGHSESLLDIST
                     RYRLVGNTIHQRTA"
     gene            6353..>6938
                     /locus_tag="VNZ06_12475"
     CDS             6353..>6938
                     /locus_tag="VNZ06_12475"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21616.1"
                     /translation="MAAQSEDSAPPLAFTREQQRDRERDFGHIQRRFDARLNAVVATL
                     LPGDYYVATEGEMISTVLGSCVSACIRDRRLRIGGMNHFMLPVDESEGQSTWGTTASS
                     TTRFGNVAMERLINSMLKLGAHRSDLEVKLVGGGKVLDAVTDIGARNIQFVREYVRAE
                     GFSVLGEDLGDVYPRKVMYHPLTGSARVKRLTRTD"
BASE COUNT         1303 a         2106 c         2304 g         1225 t
ORIGIN      
        1 ccggattgac gctgttttcg atggggctgt acctgcgtgc tgcctggccg catctgtccg
       61 cgcgagcctg agcggcaacc aattatgtga accgtgtcgc gaccggtgca cgtcctcgga
      121 gcagtgccgg gacattcgta aagaattttt gtatacacgc cgatagatcg gacgacattc
      181 cagtccatac gggtcctgga gcgcgatggg cgtgcaaagc aatttgagtt ttctggtggt
      241 ggacgatttc tccaccatgc gccgcattat caagaacctg ctcaatgacc tcggttacgc
      301 caacatcacc gaggccgatg acggcacgac ggcgctgccg atgctcaaga acggcaagtt
      361 cgatttcctg atcaccgact ggaacatgcc gggcatgccc ggcctggatc tggtcaagca
      421 tgtgcgcgcg gatgcgcgtc tgggcaagat gccggtgctg atgctgaccg ccgaagccaa
      481 acgcgagcag atcgtggaag ccgtgcaggc cggagtcaac ggctacgtca tcaaaccctt
      541 caccgccgcg acgctgaagg aaaagatcga aaagattctc gcgtcgcaga cggcggcggc
      601 ctgacctgcg atgagcggcc gattactgga caacagcgag ttcgtctcct ggctgggggc
      661 gatgtcacgc gcgctggccg agggcaacga ggaggcgctt gggaccgcac tggcgggttt
      721 tgaaggcgta cgcaacgcca ccgtcaccat gcaggtgcga cgcgttgcca gcgacctgca
      781 gttcgctctg gacgattttc acgcgaatgc gaagctggtg gacatggccc agcgccgcgt
      841 gcccgacgcg cgtcaccgcc tggcgcacgt gctgcgcctg accgatgatg ccgcgcaccg
      901 cacgatggac ctggtcgaac gcagcgcgcc gctcgccgat cacgcaaccc acgaggccga
      961 tcggctcatt caacttcagc agcagggctc cacggcgcag cagatcgcgc cgcagctgac
     1021 cgcgttcgtg acgctggtgt cggccagcat gggcgcggtt cgcagcaatc tggccgaggt
     1081 gctgatcgcg cagggctatc aggacctgac cggacagatc attcgtggcg tgatgaagct
     1141 gacgcaggag ctcgagtcct cactggccga tctgttgcgc atcgcagggc cggcgaacgg
     1201 cgaagaggcc atcaacgcga ccgcgtacgg tacccatgga ccggcggtgc cgggtgtcgt
     1261 cgaggatgcg gtcggcggtc agcaggacgt cgacgcgttg ctgtcgcagc tgggtgtctg
     1321 atgacgatcg acctgacaca gttccacgac gctttctacg aggagagctc cgaggccatc
     1381 ggccagatgg agcatgcgct gctgcgcctg gatgccggca cgcctgaccc tgaactgatc
     1441 aacacgatct tccgcgttgc gcactcgatc aagggcggcg cggcgacctt tggctttgcc
     1501 gaaatcacct cgttcacgca taccctggag acgctgctcg atgaactgcg cggtcagcgc
     1561 ctcaaggtga ccgtgacgct gtctgacctg ctgctgcgtt cgatcgacgt catgcgcgag
     1621 atgctgcgtg cgcaacaggc gcaacaaccg attgatcagc agcgcgtggc tgacctgcag
     1681 ttcgatctgg agctggccgt ggcgcagaag gtgtcggcgc cggcggcggc ctcggccccg
     1741 gagtccgcag cggtgcccgg cgtgaccagt gcgctcgagg tgcaggcgcc gctgttgccg
     1801 gagcggcatc acggcaccac ctaccagatc aatttccgtc cgtaccttca cctgctggca
     1861 cgcggcaacg atccgctgcg catgttccgc gagctgcgcg acctgggcga tctgcaggtg
     1921 acggtcaatc ttgccgcgct cccgccgatc gaggagcttg atccggaaaa ctgctatctg
     1981 tcctggcagc tgatgctcaa taccgattca ccgcgccagt cggtcatcga ggtattcgac
     2041 tgggcggagg gtgactgtga gctcgaagtc atcacgctgg atgcgccggg gagtgccgcc
     2101 gcggcgatcg cggccgcgac tgcggcgcag tctgacgggc ccggggcggc cgctgccaac
     2161 ggcgcggaag ccttgcatgc ccgagcgcac aaggcgaacg aggccgtccg ggcggtcgag
     2221 gcccagaaat cgggcgaagc cggttcgatc cgcgtcagcg tcgagaagat cgacgacctg
     2281 atgaacagtg tgggcgaact ggtgatcacg caatcgatgc tcggacaact cggcgggctg
     2341 ctggatgatg cgctcggcga gcgtctgcgc tcgggcctgg cgcaactcga gcgcaatgtg
     2401 cgcgagttgc aggagagcgt catgcgcgtg cgcatggtgc cgatcagctt tgtcttcagc
     2461 cgctttccgc gcatggtacg cgatctgagc cagcgcctga acaagcaggt cgaactgaag
     2521 atgacgggcg gcgagaccga actggacaag acggtgctcg aaaagatcgg cgacccgctg
     2581 gtgcatctgg tgcgcaacag cgtcgatcac ggtatcgaaa tgccggcaca gcgtctggcg
     2641 gccggcaagg atccgtgcgg cgtggtgcat ctgcatgcct atcaccgcgg cagcacgatc
     2701 gtggtcgagg tgacggacga tggcggcgga ctcaggcggg agcgcattct cgagaaggcg
     2761 cgcgagcgcg gtctgatcgg tcccaccgag gagctgaccg acgaggcgat cttcaatctg
     2821 attttcgtgg ctggcttctc gacccaggag cacgccaacg atgtgtccgg ccgcggcgtc
     2881 ggcatggacg tcgtcaagcg caacgttgaa gagctgggtg gcagcatcga agtgcattcg
     2941 gcacccggcg ggggatcgcg gttcacgatc acgctgccgc tgacgctcgc gatcgtggat
     3001 gggcagacca tcgccgtcgg agaggaaacc tacatcgtac cgctgacggc gatcatcgag
     3061 tcgctgcaga tgaagaccgc cgcggtgaac caggtggtcg gacacggcga ggtgctgacc
     3121 ttccgcggcg agtacctgcc ggtggtgcgg ctgcagaaac acttcaattc acgcgctgat
     3181 cgggcgcgtg aggcgagcga aggactgatc atggtggtgg agggcgacgg gcgtcgcgta
     3241 ggactgtgcg tcgatgaact gcttggtcag caacaggtgg tggtcaagac actggaggcg
     3301 aactacggtc acgtcgaggg aatcgcgggt gcgaccatcc tcggtgatgg ctcggtcgcg
     3361 ctgattctgg acgtggcgaa ccttacccgc acggtcaccg agaagctggc ggcctagggc
     3421 gtctgaaatt cgtttttgcg acgcattgtg gagacagtgt gcgctggtcg gctcaatgtt
     3481 gggcgcgcgc tgccgatagt tgtccgaagg ctcattgaaa ggtttctggc ccaatggccg
     3541 gatttgacgt aatgacggtt ggagcaggca atggctaaga caatcctcgc ggtggacgac
     3601 tccgcctcga tgcggcagat ggtaggtgtc accctgcgcg cggctggcta cgaagtcgtg
     3661 gaggcctcgg acggcgatga ggcgctcgag tatgcgcgca agcatcgcgt ggacctggtg
     3721 ctggccgacg tgaacatgcc tcgcatggat ggcatcacgc tgatcgcgca gcttcgcact
     3781 ctgcccaatt atcacctcac gccgctgctg ctgctgacca ccgagtccag ccccgagcgc
     3841 aaaatgcagg gcaagcaggc cggtgccacc ggctggatgg tcaagccgtt tcacccggaa
     3901 caactgctcg cgacgctcga gcgggtgctg ggcaatcaga ccgccgtcag cgcctgacaa
     3961 acatggacaa cggcgccaac aaagcggatg cgaagtccgg tcatagcgcg aacgtcgaac
     4021 agtttctgac gttccagctc gtcgggcagc gctacggcgt gcagatcctg caggtgcagg
     4081 agatcaaagg ttgggagaag cccacccgac tgccgcattc acccgcccat gtgcagggtg
     4141 tcatcaatct gcgcggtgcc gtggtgccga ttctggatct gcgcaagcgc ttcggactgg
     4201 gcgaggcgga atacggtcgc accaccgtcg tgatcgtcgt caaggtggag acaccgcgcg
     4261 gcgagctgac cgcgggaatg gttgtggacg gcgtctgcga ggtttgcaac atcactgcgg
     4321 aggaactgcg cgcaccgccg gaaatggcgg cggcgatcga tacggatttt gtgcgcggcc
     4381 tggcgatgat cgatgacaag atgctgatcc tgctggacgt gacgcgtctg gtcagcaaca
     4441 cgctggcgga taccgccggc accaaggctg cctgaagctc cccgttcatg gcgaactccc
     4501 ggaaatcacc gcgccgcagc tcggcggctt cccccgacga ggtcaactcc gccgcggcct
     4561 cgaagccggc ggaagcagcg gtgatcgatc tgcccgaatc gtccgcacag cccgtgaatg
     4621 tgctgacact cggcgcgtcg ctgtcgatac gcgaagtggc tgagtgcgcg gtgcagctca
     4681 aggggctgct ggccgccgga cccctggata tcgatgccgg caagctcgaa accatcgaca
     4741 cggccggcat tcagttgttg ctggcggccg cggcggccgc gcagcgccgc ggattcaaac
     4801 tcaaattgcg gggcgcccag ggactgaaga ccggcgccgc ccgctcgctg ggactgagtg
     4861 atcatctggg cgaactggcg gagatattgc catgagcgta gccgagagcc aggccctggc
     4921 cgagcagccg caggatggca gcgcgaccac cggtaccaag tttgccggcg agctgcgtcg
     4981 atttctggag ctcggtgccg cgcagctcga tgcggcgatc cgcgagagcg atgcacgggt
     5041 ggacaagctc gccggcgcgg tgacctcggt cgccacggat gcgcgtgaga ttgaaatgca
     5101 gctaccggcg ctggattcgc cgaactcggc cgagtccgag cgtgcgcgta cgcgcattcg
     5161 tcagctcacc gatgcgctgc agtcgcatgt gcaggccacg atcacgtcac tgcagtttta
     5221 cgacaagctg atccagcgcc tgacccacgt gcgtgacggt ctggcgattc cgtcggattc
     5281 gacgatgcac ggtgtagata agagctcgga ctggagcgcc atgctcgaac aggtacgttc
     5341 gcgctacagc atggtcgagg aacgggtgct gtttgacttc atgatgcgcg gcttgagtgc
     5401 cgatcagatg ctcaaagcac tcaccggtct gcggggcacg gccacgcccg gcgaactcga
     5461 agtattctga cggcggggcc atggccggcg acaccacagc catcgccggt ggggctgacg
     5521 gcaaaccatc acgcgagtac gaattctccg atcaggattt tcagcgggtg cgccgtatgg
     5581 tgcatcagca gctcggcatc gcgctgtccg agtccaagcg ccagctggta tacggacgac
     5641 tgagccgccg gctgcgcgcg ctgcatcttc gggagttcga tacctacctg cggcgcgttg
     5701 agagcggtga tgccgaagag ttgcagcact ttcgcaacgc catcaccacc aacctgacct
     5761 ccttcttccg cgaaaaccat catttcgagt ttctcgcccg gcaactgatg ccgcatctgg
     5821 aattccagaa ccgcagcagc cgccgcatcc gcatctggtc ggccggctgc tcgacgggcg
     5881 aggagccgta ttcgatcgcg atggtgctgc tggaaagcgc tccgcacctg gcacaaaact
     5941 gggacgtgcg cattctcgcg accgatatcg ataccaatgt actggaacac gccacccgcg
     6001 gcgtgtacag cagcgatcgg gtggagaaga tggattccgg gcgcctgttg cggtggtttg
     6061 cccgcaaacc cggcgaagag cagttccagg tgagcgatgc ggtcaagcaa ctggtgacct
     6121 tcagggcact caacctgatc tcggactggc ccatgaaggg gccgttcgac gtgattttct
     6181 gccgcaatgt ggtcatctat ttcgaccgtg atacccagcg tcagatcgtc ggccgcatgg
     6241 aagggctgca gcgccccggc gactatctga ttctcggaca ctccgaatcg ctgctcgaca
     6301 tcagcacccg ctatcgcctg gttggcaaca cgattcacca acggacggcc tgatggccgc
     6361 gcagtcggaa gactcggctc cgccgctcgc cttcacccgt gagcagcaac gcgaccgcga
     6421 acgcgatttc ggtcatatcc agcgacgctt cgatgcacgt ttgaacgccg tggtggcgac
     6481 tctgcttccg ggcgactact acgtcgcgac cgagggcgag atgatctcca ccgtgctcgg
     6541 ctcgtgcgta tcggcctgca ttcgcgaccg ccgcctgcgt attggcggaa tgaaccactt
     6601 catgttgccg gtcgacgagt ccgagggcca aagcacctgg ggtacgaccg ccagctccac
     6661 tacgcgcttc ggcaatgtcg ccatggagcg actcatcaac tcgatgctca agcttggtgc
     6721 acaccgcagt gacctggaag tcaagctggt gggcggcggc aaagtgttgg atgcagtgac
     6781 ggacatcgga gcgcgcaata tccagttcgt acgagaatat gtgcgcgccg agggtttttc
     6841 ggtactggga gaggatctgg gtgacgtgta tccgcgcaag gtcatgtatc accccctgac
     6901 cggaagcgcg cgcgtcaagc ggctgacccg caccgacc
//
LOCUS       DATMRM010000411         5043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4277999, whole genome shotgun sequence.
ACCESSION   DATMRM010000411 DATMRM010000000
VERSION     DATMRM010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4277999"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VNZ06_12480"
     CDS             complement(<1..477)
                     /locus_tag="VNZ06_12480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWW21617.1"
                     /translation="MIELAGNAVAVTGAASGIGRALAQQLAQRGCDLALADVDEVGLK
                     SVSDELIRAGRVNVLARRVDVANAAQVHEFAREAILSFPKLCVVINNAGVALQGQFEE
                     LDLAQIQWLMGINFWGVVHGTHAFLPHLQRQSWAHIVNISSIFGIIGPPGNCAYAAS"
     gene            complement(588..2048)
                     /locus_tag="VNZ06_12485"
     CDS             complement(588..2048)
                     /locus_tag="VNZ06_12485"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HWW21618.1"
                     /translation="MKTRVFVRAAVLGAALLPLGALASSHREAPTIAGLPQLDGTDFY
                     MFRSYEPGRSAFVTLIANYFPLQDPFGGPNYFNLDPNATYSIHIDNDGDAKSDITFEF
                     NFRTIFKDLTVNANGVQTSIPLILAGPIDVRGDNANVTQDYTVTVVRNGERQLASNET
                     NGGHTFFRPVDNIGNKTFPDYDMYASQFVAQIAIPGCNTPGRVFAGQRHEGFIVNLGP
                     TFDLVNLNPVGPRDGNPNNVTNKNITSLAIEVPIACLTNGKDPVIGGWTTSSVRDGDG
                     DDGRRQTSRLGSPLVNEVVIGLQDKDKFNASKPINDAQFAHYVTNPYLPVLLNAEFGN
                     AALIPQSPRDDLVAIFLTGIKGLNQPLKVTPSEMLRLNTSIPVTAPSAQNDLGVLAGD
                     LAGYPNGRRPYDDVVTIELRAAEGAVCGAVGNCGSETSDPNRGNPYTDGARAAGPDVA
                     DSHVTGAINAQDTYLPTFPYLNNPLPGSPDGANGVH"
     gene            complement(2181..3089)
                     /locus_tag="VNZ06_12490"
     CDS             complement(2181..3089)
                     /locus_tag="VNZ06_12490"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWW21619.1"
                     /translation="MHIERNELRCFHAVLDAGGFSRAAERLDLSQSAVSQAIANLEHR
                     LGASLLHRGNPPQPTEAGLRLLRFAQTVLNEERETLADINQIKSGALSTLSLALSPAA
                     NSKIGIALLKEFCERNPLTRLKVVVAPSREIVHGVAEGRWELGFGPFHHNMPAHFALH
                     PCLSEVRRLMIARDHPARAALLRDPVGTVRTLPLVTSSLDESARRASGERLRDSFGSV
                     WEVSHMELRLALVADGKGVTYVSDLFAAIPQQLVPVQGLPYSEIERQVGAYCLKHQPL
                     SQAGARFLALCHERWPRHRESQASVS"
     gene            3219..4352
                     /locus_tag="VNZ06_12495"
     CDS             3219..4352
                     /locus_tag="VNZ06_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HWW21620.1"
                     /translation="MNAVMESKPDIKKGLEGVIVDTTSTSLVDGEAGRLYYRGYPIER
                     LVQHPFAAVAHLIVFGDLPAAPRLREFEEFLWQAGKLPSDLAATVAQLSRLGGHPMAT
                     LQAAMPLMACEPPAVTMGRGVDEQEALLVAARLPAAHALLLALRTGRTPTTYPDTRSY
                     GERFLQLLHNRKPDARAVNIFEQTQVLQIDHGFNASTFTARVVTSTLAPPASALAAAM
                     GSLYGPLHGAADQGALEMAEEVGDPTHADAFVAKCLAEGRLVMGMGHREYRVVDPRSK
                     IVKILAEQIASDAHQRRLIATLKAVDEAFVTQTAHKKRSLRANLEFYKGVVYLGLGLP
                     KEFFTATFAIARIFGWSAHLVEQRKDNRIVRPASHYIGPAPQA"
     gene            complement(4432..>5043)
                     /locus_tag="VNZ06_12500"
     CDS             complement(4432..>5043)
                     /locus_tag="VNZ06_12500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW21621.1"
                     /translation="CDNGVGIPAELLPHVFDLFVQSDRSLDRSLGGLGIGLAVVKRLV
                     EMHGGRVSASSEGLGKGARFEIRLPVTRPLAAVEVAAPRAMIAPRRILVVDDNVDAAV
                     SLRHLLELDGHQSLAVYGAQEALESVSSFRPEIVLLDIGLPHMNGYEVARRLRACDGG
                     PSLKLIALTGYGQSEDRLKALAAGFDAHLAKPVDLGALERLLT"
BASE COUNT          978 a         1572 c         1548 g          945 t
ORIGIN      
        1 cgaggccgcg taggcgcaat tgcccggcgg cccgatgatt ccgaagatgc tcgatatatt
       61 gacgatgtgc gcccaggatt gacgttgcag gtgcggcaga aacgcatgcg tgccgtgcac
      121 cacgccccag aaattgatcc ccatcagcca ctggatctgc gccaggtcca attcctcgaa
      181 ttgtccctgc agcgcgacgc cggcgttatt gatcacgacg cacagtttcg gaaagctcaa
      241 aatcgcttca cgagcgaatt cgtgcacctg agccgcgttg gcaacgtcaa cgcggcgcgc
      301 tagaacgttt acccgaccgg cgcggatgag ttcgtcagac accgatttga gaccgacctc
      361 atcgacatcg gccagcgcca gatcgcagcc gcgctgcgcg agctgctgcg ccagtgcgcg
      421 cccgataccg ctggccgcgc cggtcacagc cactgcattg ccggcaagct caatcatcgc
      481 tgctcacccc tcctttggaa tcgagcatgt taccggaaat agaaaaggcg ccgcggatct
      541 cacgatccgc gacgccagct tggaatcaac tgaggttgat cttgagctca gtgcacgccg
      601 ttggctccgt cgggtgaacc cggcagcgga ttgttcaggt acgggaacgt cggcaggtag
      661 gtatcctgcg cgttgatcgc gcctgtcaca tgcgagtcgg ccacatccgg accggcagca
      721 cgcgcaccgt cggtataagg attgccgcgg ttcggatcac tggtttccga gccgcagtta
      781 cccaccgctc cgcacaccgc gccttcggcc gctcgcagct cgatcgtcac gacgtcgtca
      841 tagggacggc ggccgttcgg atagccggcc agatctccag cgagtacacc gaggtcgttc
      901 tgagccgagg gcgcagtgac cgggatggag gtgttcaggc gcagcatctc gctcggcgtg
      961 actttcagcg gctgattcag tcccttgatg ccggtcagga agatcgcgac caggtcatcg
     1021 cggggcgact gaggaatcag tgcggcattg ccgaactcgg cgttcagcag cacgggcaga
     1081 tacggattgg tcacgtagtg cgcgaactgt gcgtcgttga tcggcttgct ggcgttgaac
     1141 ttgtccttgt cctgcaggcc gatgaccact tcattgacca gcggtgagcc cagacgcgag
     1201 gtctggcgcc ggccgtcatc gccatcgcca tcgcgcacgc tggaggtggt ccagccaccg
     1261 atgaccggat ccttgccatt ggtgaggcag gcaatcggca cttcaatggc cagcgacgtg
     1321 atgttcttgt tggtcacgtt gttgggattg ccgtcacgcg gtccgaccgg attcagattg
     1381 accaggtcga acgttgggcc gaggttcacg ataaaccctt cgtgacgctg accggcgaac
     1441 acacgtcctg gcgtattgca gccggggata gcgatctgcg cgacgaactg gctggcatac
     1501 atgtcgtagt ccggaaaggt cttgttaccg atgttgtcga ccggacggaa gaacgtgtgg
     1561 ccaccgtttg tctcgttgct cgccagctgt cgctcgccgt tgcgcaccac ggttacggtg
     1621 tagtcctgcg tcacattggc gttgtcgcca cgcacgtcga tcgggccggc cagaatcagc
     1681 ggaatcgaag tttgcacgcc gttcgcgttg accgtcaggt ccttgaaaat cgtcctgaag
     1741 ttgaactcga aggtgatgtc cgacttcgca tcgccatcat tgtcgatgtg aatgctatag
     1801 gtcgcgttgg ggtcgagatt gaagtagttc ggtccgccga atggatcctg cagcggaaag
     1861 tagttggcaa tcagcgtgac aaatgcgcta cgaccaggct cgtagctgcg gaacatgtag
     1921 aaatcggtgc cgtcgagttg cggcagaccg gcaatcgttg gtgcttcgcg atggctggat
     1981 gccagagcgc cgagtggcag tagcgccgct cccagcacag ctgcgcgtac aaagactctc
     2041 gtcttcatag agaatgtccc ctagatgagt gaataagtgg gcgttaagac gcggattgct
     2101 ccccgatctc atcagttcta acgcctgggg atagcgctcg gatgcatgcg gcgagcaaga
     2161 aaagtcgccg gcaggaattg tcaggaaacg ctagcttgcg actcgcgatg tcgtggccag
     2221 cgttcatggc agagcgcaag aaagcgcgcg ccagcctgcg acaacggttg atgtttcagg
     2281 cagtaagctc cgacctggcg ttcgatctcc gaatacggca ggccctgtac cggaacgagc
     2341 tgctgaggga tggcggcgaa caggtcggac acataggtga cgcccttacc gtccgcgacc
     2401 agggccagtc gcagctccat gtgactgact tcccagaccg agccgaatga atcgcgcaga
     2461 cgctcgccgc tggcgcgacg agcggactca tccagtgatg aggtgaccag cggcagggtg
     2521 cgcaccgttc ccaccggatc gcgcaacagc gcggcgcgtg ccggatgatc gcgcgcgatc
     2581 atcagccgac gcacctccga caggcacgga tgcaaggcga agtgcgccgg catgttgtga
     2641 tgaaacgggc caaagcccag ttcccagcgc ccttctgcca cgccgtgaac gatctcgcga
     2701 ctcggagcca ccaccacttt caatcgcgtg agcggattgc gctcgcagaa ctccttcagc
     2761 agcgcaatgc cgattttcga gttggccgcc ggtgagagag cgagcgacag cgtcgacagc
     2821 gcgccggact tgatctgatt gatgtccgcc agcgtctcgc gctcctcgtt gagcacggtc
     2881 tgcgcgaagc gcagcaacct cagaccggcc tcggtcggct gtggcgggtt tccacgatgc
     2941 agcagactgg ctcccagacg gtgctccaga ttcgcaatcg cctggctgac tgccgactgc
     3001 gacagatcga gccgctcggc agcgcggctg aaaccacccg catcgagcac cgcgtgaaag
     3061 cagcgcagtt cattacgttc aatgtgcatg cgtcaaagga tagcccttac ggcggcgatt
     3121 cgtctgcgtt atccgtctgt ctgatagccc gatcacttga gcgctctggc ccctatcagg
     3181 cgttcgccat agcctttgca ctatcaagga gaccccccgt gaatgccgtg atggaatcca
     3241 aacctgatat caagaaaggc cttgaaggcg tcatcgttga caccacctcg acatcgctgg
     3301 tcgacggcga ggccgggcgg ctctactacc gcggctatcc gatcgagcgc ctggtacaac
     3361 atccgtttgc tgcagtggct cacctgatcg ttttcggtga tctgcctgct gcaccgcgac
     3421 tgcgagaatt cgaggaattt ctgtggcagg ccggcaagct gccgtccgac cttgccgcca
     3481 ccgtcgcgca gctctcgcgc ctgggcggac atcccatggc gaccctgcag gctgccatgc
     3541 ccctgatggc atgcgagccg cccgccgtca cgatgggacg cggcgtggat gaacaggagg
     3601 cgttgctggt cgcggcacga ctgccagccg cccacgcatt gctgctggcg ctgcgcaccg
     3661 gtcgcacgcc gacgacctat ccggacaccc gcagctacgg cgagcgcttt ctgcagttgc
     3721 tccacaaccg caagcccgat gcgcgcgccg tgaacatatt cgaacagacg caggtgctgc
     3781 agatcgatca cggtttcaac gccagcacct ttacggcacg ggtggtcacg tccacgctcg
     3841 cccctccggc ctcggcactg gcggcggcga tgggttcact ttatggaccc ttgcacgggg
     3901 cggccgatca gggcgcgctc gagatggccg aagaagtggg cgatccgacg cacgccgatg
     3961 cgttcgttgc caagtgcctg gccgagggcc ggctggtgat ggggatgggc caccgcgaat
     4021 accgcgtggt cgatccacgt tcgaagatcg taaagattct ggccgaacag attgcgagcg
     4081 acgctcatca gaggcggctg atcgcgacac tcaaagcggt agacgaggcg ttcgtcacac
     4141 agacggccca caagaaacgc tcactgcggg ccaatctgga gttctacaaa ggcgtggtgt
     4201 acctagggct agggttgccg aaagagttct tcacagccac gttcgcgatc gcccgcatct
     4261 tcgggtggtc cgcgcatctg gtggagcaga gaaaggacaa tcgcatcgtc cgacccgcgt
     4321 cacattacat cggacccgca cctcaagcgt gagattgacg gtacgcgcgt gcatgctgtt
     4381 ctcggaagac cctggccacc atcagggcac gaaccgatgc ctgatgatgt atcaggtcag
     4441 caggcgctcc agggcgccga ggtccaccgg cttcgccaga tgggcatcga agccggcagc
     4501 cagcgccttc agtcggtctt cgctttggcc gtaaccggtc agggcaatca gtttcaggct
     4561 cggaccccca tcgcaggcac gcagccggcg cgcgacctcg tagccgttca tgtgcggcag
     4621 gccgatatcc agcaacacaa tttccggtct gaagctgctc acactctcga gcgcttcctg
     4681 cgcgccgtac accgccagcg attggtgtcc atcgagttcc agcaggtgcc gcagcgacac
     4741 cgcggcatcg acgttgtcgt cgaccaccag aatgcggcgt ggcgcgatca tcgctctagg
     4801 cgcggcgacc tccaccgctg cgagcggccg tgtcaccggc agacggatct caaagcgggc
     4861 acctttgcca agaccttcac tgctggcgct tacacgtcct ccatgcattt cgaccaggcg
     4921 tttcaccact gccaatccaa ttccaagtcc gccgagcgac cgatcgagcg agcgatcact
     4981 ctgcacgaac agatcaaata cgtgcggtag cagctcggcg ggaattccca cgccgttgtc
     5041 gca
//
LOCUS       DATMRM010000412         4981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3320309, whole genome shotgun sequence.
ACCESSION   DATMRM010000412 DATMRM010000000
VERSION     DATMRM010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3320309"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..881
                     /locus_tag="VNZ06_12505"
     CDS             306..881
                     /locus_tag="VNZ06_12505"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HWW21622.1"
                     /translation="MPHDPSETVTKLLDHWAQGDQQALAALLPLVYNELRRVAHYQLQ
                     RERPDHTLQSTALVHEAYLRLLGNKAPELQGRSHFIAISSRLIRQILVDYARERRAAK
                     RDGGCRVAMELIDAAPVLQDADLIALDEALNDLYRTDERQAKIVDMKFFGGLSSPEVA
                     DVLGMSRATVDRDWSTARAWLHRQIQRTASS"
     gene            878..4603
                     /locus_tag="VNZ06_12510"
     CDS             878..4603
                     /locus_tag="VNZ06_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWW21623.1"
                     /translation="MTDERWERVKDLLHQALRLTEDQRSAFLGEVCRADEPLRRELES
                     LLAADAQANPNFLRSGMHLEALELPDGIDSAHSLIAGHIFEQRFRLLRALGEGGMGQV
                     WLAEQFVPVRREVALKLIRAGMYDEAVLQRFRAERQSLAIMDHPYIAKVFEAGSTAQG
                     QPYFVMEYVPGLAITEYCDHKRLAIPERLELFMKACEGVQHAHQKAIIHRDLKPANIL
                     VIEVDGVPTPRIIDFGLAKPLADASAERTMPTQFGQFLGTPGYMSPEQADPIAAKDID
                     TRTDVYSLGVILYVLLTGSRPFDAHHEAPQPFDEWLRQLRTVDPPRLTALLTRDHVDS
                     AAAARASHTGQLVRQLRGDLEWIASKAVWRERGSRYASPAQFAEDLRRYLCHETVSAR
                     PASVTYQVRRFIRRHRLVATFAALISISAIAAAIAGLLAVRRQHEAEYQQRQAQYQTQ
                     QALQAQRHLLTEVAAERLANRDVAGAQQIIVELLTHPSLPQERSPAAISVFQMTRAAD
                     TEIAVLSGHTARVLSARYSPDGARIVTGSGDNTARVWDAHSGAALLMMSGHTDRVFDA
                     AYSPDGTRIVTGSHDKTARVWDARTGSLQLTLNGHGDRVDSARFSPDGQRIVTASWDK
                     TVRIWAAASGKLLTTLTGHSDAVYSAVYSGDGRRIVTASQDKSARIWDATSGKPLAVL
                     TGHGDYVACAAYSADGQRIVTASADRTARVWDARSGAPLAVLSGHGDVVYSAAFSPDG
                     RRIVTASWDKSARIWDASTGRQLAVLAGHQDTLASAEYSPDGAYLVTASQDHTARIWD
                     ARVSMELTTLSGHRDAVYSAAYSPDGRRIVTASNDKTARIWDARTAETLQTLSGHAGA
                     IDFAQYSHDGRRIATASQDRSARIWDATSGKQLVGLSGHTDRVYSVAFSPDDARVVTA
                     SRDKSARLWNALTGAAQAVLFGHTDRVYSGAWSHDGQRIVTASRDRTARIWDAAKATT
                     LHTLQGHDDQIVTAAYSPDDSRIVTASSDGTARIWDARSGAQLTVLTGHGGALTSAAY
                     SPDGQQIVTTSEDRTARVWNAQSGEQLAVLAGHAAAVLFAAYSPDGTRIVTASEDQTA
                     RIWDARPPGNLAMQIQWYAAAEIAPMSDGERLQLGLPRDPRVTTSTPERAAASSGAVS
                     GPDEPEALARRAEQEELAAIGETEPALRDQRLLRALTLYATAAERARLQGRAGDRYQH
                     WCYRRGSLARVLAREGWMQQAAESYREALQRARNSGG"
     gene            complement(4559..>4981)
                     /locus_tag="VNZ06_12515"
     CDS             complement(4559..>4981)
                     /locus_tag="VNZ06_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWW21624.1"
                     /translation="EYSRSLWMRDRRDSVPGVHLPETSARKFPRAAESWVWHWVFPSA
                     TLSNDPRSGLCRRHHQYEQGVSRAITRASHRAGITRRVTAHTLRHSFATHLLESGVDI
                     RRIQQLLGHSDVSTTMIYTHVLGSSAAGVASPLESLSI"
BASE COUNT          912 a         1625 c         1575 g          869 t
ORIGIN      
        1 catcgctgcg acatcctcga gaccggcaac gacagctatc gcttcaagaa gcgcaaatga
       61 gcgacagaag atctgccgct gacgcggcag actgcgctgc ggcttgagac ctcaaaggaa
      121 gccaaagacc aaaaccccgg aacgaaagac cggacgcgcc attacccctg gtcaatcttc
      181 aacgccgaaa gctggtcaat gttcaacgcc ggttgacagt tagcgatgcc gcaagccttt
      241 gatttgacta gcttgtggac cttacctata gtcggttgcc acgccttgcg gataggccat
      301 cccccgtgcc ccacgatccg tccgagaccg tcacgaagct gctggatcat tgggcccagg
      361 gcgatcagca ggcgctcgcc gcgctgctgc cactggtcta caacgaactg cgacgcgtgg
      421 cgcactatca actacagcgc gagcgaccgg atcacacgct gcaaagcacg gcgctggtgc
      481 acgaggcgta tctgcgcctg ctgggcaaca aggcgcccga gcttcagggg cgctcacact
      541 tcatcgcgat ttcctcgcgg ctgattcgtc agatcctggt cgactatgcg cgcgaacgtc
      601 gcgccgccaa acgcgatggc ggttgccgcg tggcgatgga gctgatcgat gcggccccag
      661 tgctgcaaga cgcggatctg atcgcgctcg atgaggcgct gaatgatctg tatcgaaccg
      721 acgaacggca ggcgaagatc gtcgacatga aattctttgg cggtctgtcc tcacccgagg
      781 tcgctgacgt ccttgggatg tcgcgcgcaa cggttgatcg cgactggtca accgcccggg
      841 cgtggttgca tcggcagatt caacggaccg catcgtcgtg accgacgagc gctgggagcg
      901 cgtcaaggat ctgctgcacc aggcattgcg gttgaccgag gatcagcgca gcgcgtttct
      961 tggcgaggtg tgtcgtgccg acgagccgct gcgccgcgag cttgaatcgc tgctggcagc
     1021 cgacgcgcag gcaaacccca attttctgcg ctccggcatg cacctcgagg ccctggagct
     1081 gccggatggc atcgactccg cgcacagcct catcgccggt catattttcg aacagcgctt
     1141 tcggctgctg cgggcgctgg gcgagggagg aatggggcaa gtgtggctcg ccgagcagtt
     1201 cgtgccggta cgacgcgagg tcgcgctgaa gctcattcgc gccggcatgt acgacgaggc
     1261 ggtattgcag cgcttccggg ccgagcgtca atcactcgcc atcatggacc atccttacat
     1321 cgccaaggtg ttcgaggccg gtagcaccgc acaaggccag ccctactttg tgatggaata
     1381 cgtgccaggg cttgcgatta cggaatactg cgatcacaag cggcttgcca tcccggagcg
     1441 gctcgagttg ttcatgaagg cgtgcgaggg cgtgcagcac gcgcaccaga aagcgatcat
     1501 tcaccgcgat ctgaagccgg ccaacatcct ggtgatcgaa gtggatggcg tgccgacacc
     1561 gcgcatcatc gattttggtc tggcgaagcc attggcggac gcctccgccg agcgaacgat
     1621 gcctacgcag ttcggccagt ttctcggcac ccccggctac atgagcccgg agcaggccga
     1681 cccgattgcg gcgaaggaca tcgatacccg caccgatgtc tattctctgg gggtgatcct
     1741 gtatgtgctg ctgacaggat cgaggccatt tgacgctcat cacgaggcac cccagccatt
     1801 tgatgagtgg ctgcggcaac tgcgcaccgt cgacccgccg cgtctgacgg cattgctgac
     1861 ccgggaccat gtggatagcg ccgctgcagc gcgcgcgagc cataccggac agctggtgag
     1921 gcagcttcgc ggcgatctcg agtggatcgc gtcgaaagcg gtgtggcgcg agcgcggcag
     1981 ccgctatgcc tcgccagcac agtttgccga agacctgcga cgctacctgt gccacgaaac
     2041 agtgagcgcg cggcccgcaa gcgtcactta tcaggtgcgt cgattcattc gccggcatcg
     2101 tctggtcgcg acgttcgccg cgctgatcag catctctgcg atcgcggcgg cgattgccgg
     2161 cctgttggcg gtacgccgtc agcatgaggc cgagtatcaa cagcgtcagg cccaatacca
     2221 gacgcaacag gcgctgcagg cgcagagaca tctgctcact gaagtggctg ccgagcgcct
     2281 cgccaaccga gatgtggccg gtgcgcagca gatcatcgtc gaactgctca cgcatcccag
     2341 cctgccgcag gagcgctcgc cggccgcgat cagcgttttt cagatgaccc gcgctgcgga
     2401 tactgagatt gcggtcttgt caggacatac ggcgcgcgtg ctatcggcgc gatattcgcc
     2461 ggatggcgct cgcatcgtga ccggctcggg cgacaacacc gctcgcgtct gggatgcgca
     2521 cagcggtgcg gcactcctga tgatgagcgg acacaccgac cgcgtgttcg atgcggccta
     2581 ctcgccggat ggcacgcgga tcgtcacggg ctcgcacgac aagacggcac gcgtgtggga
     2641 tgcgcgcacc ggcagcttgc agctgaccct caatgggcac ggtgatcgcg tcgactcggc
     2701 gcgcttttcc cccgacggcc agcgcattgt caccgcctcc tgggacaaga cggtgcgcat
     2761 ctgggccgcc gccagcggca agctgctgac cacactgacc gggcacagcg atgcggtgta
     2821 ctcggccgtc tactccggcg atggccgccg tatcgttacc gcctcccagg acaagagcgc
     2881 tcgcatctgg gatgccacga gtggcaaacc gctagccgtc ctgaccgggc acggcgacta
     2941 cgtcgcctgc gctgcgtatt ccgctgacgg acagcgcatc gtcaccgcct ccgccgacag
     3001 gaccgcccgt gtctgggacg cccgctcggg agcgccgctc gcggtgctat ccggacatgg
     3061 cgatgtggtc tattcggccg ccttctcacc cgacggccgg cgcatcgtca cggcttcatg
     3121 ggacaagtcc gctcgcattt gggatgcgag cacgggtcgg caactggcgg tgctcgcagg
     3181 ccatcaggac acattggctt ccgctgagta ctcaccggat ggcgcatacc tcgtcacggc
     3241 gtcccaggac cacactgccc gcatctggga tgcgcgggtg tcgatggagc tgacaacgct
     3301 ctcgggacat cgcgatgcgg tttactccgc cgcctactca ccggacggcc gccggatcgt
     3361 cacggcatcc aacgacaaga ccgcgcgtat ctgggacgcg cgcaccgctg aaacactgca
     3421 aacgttatcc ggacatgcgg gcgcgatcga ttttgcgcag tactcgcacg acggccggcg
     3481 catcgcgacg gcctcccagg atcgcagcgc gcgcatctgg gatgccacca gcggcaaaca
     3541 gctcgtcggt ctgtcaggtc acaccgatcg tgtctacagc gttgcattct cgcctgatga
     3601 tgcgcgcgtc gtcaccgcct cgcgtgacaa gagtgcgcgt ctgtggaatg cactcaccgg
     3661 cgctgcgcag gcggtgttgt tcggccacac cgatcgcgtc tactctggag cctggtcgca
     3721 cgatgggcag cgcattgtca cggcatcgcg cgatcgcacg gctcgaatct gggacgccgc
     3781 caaagcgacc acgctgcaca cgctccaggg ccacgacgat cagattgtga cggccgccta
     3841 ttcgcccgac gacagtcgaa tcgtgacggc ctcgtccgat ggcaccgcga ggatctggga
     3901 cgcgcgctcc ggtgcgcagc tcacggtgct gacaggccat ggcggggcac tgacctcggc
     3961 cgcgtactcg cccgatggcc aacagatcgt gacgacttcc gaggatcgga ccgcacgggt
     4021 ctggaacgcg cagtcgggcg agcaactcgc ggttcttgcc ggccatgcgg cggctgtcct
     4081 gtttgccgcc tactcacccg atggcactcg catcgtcacg gcatcggaag atcagaccgc
     4141 gcgtatctgg gatgcgcgcc ctcccgggaa tcttgcgatg caaatccagt ggtatgcggc
     4201 tgccgagatc gcgcccatgt ccgacggcga acgtcttcag ctcgggctac cacgcgatcc
     4261 gcgcgtcacg acttcaactc cagaacgcgc agcggccagc tctggcgccg tctcaggccc
     4321 tgacgaaccc gaggcgctgg cgcgtcgcgc ggagcaggag gagctagccg ccatcggcga
     4381 gaccgaaccg gcgctccggg atcagaggct gcttcgggca ttgaccctgt acgctaccgc
     4441 cgctgagcgg gcgcgtctgc agggacgagc cggcgatcgc taccaacact ggtgctatcg
     4501 gcgcggctcg ctggctcgag tgctcgctag agagggctgg atgcagcagg cggctgaatc
     4561 atatcgagag gctctccagc gggctcgcaa ctccggcggc tgacgaaccg agaacgtggg
     4621 tgtagatcat cgtcgtgctg acgtcgctgt gacccaacag ctgctgtatg cgacgaatat
     4681 ccactcccga ttcaagcagg tgcgtcgcaa acgaatgccg cagcgtgtgc gcagtcacgc
     4741 gcctcgtgat ccccgctcga tggcttgcgc gagtgatcgc tcggctcaca ccctgctcat
     4801 attgatgatg ccgccgacac agtccggatc gaggatcatt cgacagcgtc gccgacggaa
     4861 acacccaatg ccacacccag ctttcggcag cacgtggaaa ttttcgtgcg ctcgtttcag
     4921 gcaggtgaac accgggcacc gagtctcttc gatctcgcat ccatagggag cgcgaatact
     4981 c
//
LOCUS       DATMRM010000413         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1493989, whole genome shotgun sequence.
ACCESSION   DATMRM010000413 DATMRM010000000
VERSION     DATMRM010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493989"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VNZ06_12520"
     CDS             complement(<1..522)
                     /locus_tag="VNZ06_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HWW21625.1"
                     /translation="MVFNSLTFVVFFACVLALHALPLPWTTKKINLLIASYLFYAAWN
                     PPFVILLWVSTVVDWYAAQGLVKAQRAATRHAWMLLSVIANLGLLGYFKYGQFLLDNF
                     TSVMSAVGIAYHPAHYDIVLPVGISFYTFATMSYTLDVYLRRAAPAQNLLDYALFVTF
                     FPHLVAGPIMRPTE"
     gene            complement(639..1115)
                     /locus_tag="VNZ06_12525"
     CDS             complement(639..1115)
                     /locus_tag="VNZ06_12525"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWW21626.1"
                     /translation="MATNPIPDGYRTVTPYLIIKGAAEAIEFYKRAFGATELLRMADP
                     QGRVGHAEIKIGDSVIMLADEQPAMGYRGPRSLGGSSVSILLYLEDVDAVFERAVRAG
                     ARAQRPVADQFYGDRSGTLEDPFGHVWTVATHVEDVPAEEMQRRAEAAMKSAAPAA"
     gene            complement(1208..>2001)
                     /locus_tag="VNZ06_12530"
     CDS             complement(1208..>2001)
                     /locus_tag="VNZ06_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWW21627.1"
                     /translation="WGVWVAVIIAIFSYFSIEMIAVAAGEAAQPEIAISRAFRATVFR
                     LILFYLLTLALVLAIVPWTAAGIEESPFVKVMRALRVPGAAGIFNLVILVAALSAMNS
                     QLYITTRMMFSLSRANQAPRRFGLLNRRGVPVQALLLSSIGITVAALLSIVAPKSAFI
                     LMVSISAFGTMFAWMMIFVTHYRFRRVQARTGAAPLRFRMPGFPATTLLGATLMAAVL
                     LTTALTEAFRMTLVFGVPFLLCLTVTYWIWYRRNARTAVSSAGAA"
BASE COUNT          406 a          654 c          642 g          299 t
ORIGIN      
        1 ctccgtcggg cgcatgatgg gaccggccac caggtgcggg aagaacgtca cgaacagggc
       61 gtaatcgagc aggttctgcg ccggcgccgc gcgccgcagg tacacgtcca gcgtgtacga
      121 catggtcgcg aacgtataga aggagatgcc gacgggcagg acgatgtcgt agtgggcggg
      181 gtgataggcg atgccgaccg ccgacatcac cgaagtgaag ttatcgagca ggaactgtcc
      241 gtatttgaaa taccccaaca ggcccaggtt cgcgatcacc gacagcagca tccaggcatg
      301 ccgggtcgcg gcgcgctgcg ccttcaccag tccctgcgcg gcataccagt cgaccacggt
      361 cgacacccac agcaggatca cgaacggcgg attccacgcc gcatagaaca ggtagctcgc
      421 gatcaggagg ttgatttttt tcgtggtcca gggcagaggc agcgcgtgca gcgcaagcac
      481 gcacgcgaag aacacgacga acgtgagcga gttgaaaacc atgatggccc gccgcccgcg
      541 cgcacgcgga ctggcagtct ctcccccggc acgcccctat ggggcttttg ggcatcatgt
      601 acgagcaggg ccgcgtcagg caaatcgccc cggcgacctc aggcggcggg cgccgccgac
      661 ttcatggctg cctcggcacg ccgctgcatc tcctccgccg gcacatcctc cacgtgcgtg
      721 gcgaccgtcc agacgtgacc gaacggatct tccagcgtgc ccgagcggtc accgtagaat
      781 tgatccgcca ccggccgctg cgccctcgcc ccggccctga cagcgcgttc gaacacggcg
      841 tccacatcct cgaggtacag caggatgctg acgctcgatc caccgagcga gcgtgggccg
      901 cggtagccca tcgcgggttg ctcgtcggca agcatgatca cggagtcgcc gatcttgatc
      961 tcagcgtgac cgactctgcc ctgcgggtcg gccatgcgca gcagctccgt cgcaccgaag
     1021 gcgcgcttgt agaactcgat cgcctcggcc gcacccttga tgatcaggta cggggtcacc
     1081 gtgcggtagc catcgggaat cgggtttgtg gccatgggtt tggctcctcc attcttagtt
     1141 ccgcagctta ctccggggca ggtggcaaat taatgacacg cgcaaggcag cggcacccgt
     1201 ccagtgctca ggcagcgccg gcactggaga cagcggtgcg cgcgttgcgc cggtaccaga
     1261 tccagtacgt caccgtgaga cacagaagaa acggcacacc gaacaccagc gtcatgcgga
     1321 acgcctccgt gagggcggtc gtcagcagca cggctgccat gagcgtggcg ccaagcagcg
     1381 tcgtggcggg aaagcccggc atgcgaaagc gcaacggcgc agccccggtc cgcgcctgca
     1441 cgcggcggaa gcgatagtgg gttacgaaaa tcatcatcca ggcgaacatg gtgccaaagg
     1501 ccgagatcga caccatgaga atgaacgccg acttgggcgc gacgatgctc agcagcgccg
     1561 ccaccgtgat gccgatgctc gagagcagca gtgcctgtac cggcacgccg cgccggttca
     1621 ggagaccgaa gcgacgcggc gcctgattcg cccgtgacag gctgaacatc atccgcgtgg
     1681 tgatgtagag ctggctgttc atcgccgaca gtgccgcaac caggatgacg aggttgaaaa
     1741 tgcccgcggc gccaggcacc cgcagcgcgc gcatgacctt cacgaacggg ctctcctcga
     1801 tgcctgccgc ggtccacggc actatcgcca gcaccagggc gagggtcagc agatagaaaa
     1861 ggatcagccg gaataccgtc gcacgaaacg cgcggctgat ggcaatctcg ggctgcgcag
     1921 cctcgcccgc ggcgacggcg atcatctcga tgctgaagta actgaatatt gcgatgatca
     1981 ccgcgaccca tacgccccac a
//
LOCUS       DATMRM010000414         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3637843, whole genome shotgun sequence.
ACCESSION   DATMRM010000414 DATMRM010000000
VERSION     DATMRM010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3637843"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VNZ06_12535"
     CDS             <1..615
                     /locus_tag="VNZ06_12535"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HWW21628.1"
                     /translation="TALRCGLLSCHSCGLLVKSTAHVHGAACPRCGAHLHARKPDSIA
                     RTWAFLIAAIVLYIPANALPVMNTSSLFGSEKDTILSGVVYLWTSGSWPLAAVVFIAS
                     IAVPMLKILALMYLVISTQRRSVMLLQQRTRIYRLVELVGRWSMLDIYVITILVALVQ
                     FNALATIQAGPAAVAFGSVVVLTMFAAMSFDPRLIWDAMEDHHV"
     gene            707..2218
                     /locus_tag="VNZ06_12540"
     CDS             707..2218
                     /locus_tag="VNZ06_12540"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWW21629.1"
                     /translation="MLIGGWLAVKAVLEQGPTVTISFTTGEGLEAGKTKIKFKNVDVG
                     VVKSVTLSRDHRSVVATAELVKDASNMLVDDTRFWVVRPRISGGTVSGIGTLLSGSFI
                     GMDLGASTKSRRDYVGLETPPVFASGVPGREFVLKSEDMGSLDIGSPVFFRRLQVGQI
                     TSYSLDADGKGVTLRVFVNAPYDKYVQDDTRFWHASGVDVSLGTDGVKVNTQSVVAIL
                     IGGLAFQTPDETTGQSEAAVRTEFMLFHDRAEAMKRHDRIIESYVFNFNDSVRGLTVG
                     APVDFRGIVVGEVAAIYTRFDQAKKQFSIPVEVRLYPERFTSRYQSGGSGGRLSSDPH
                     RMAEWLVSQGLRAQLRTGNLLTGQLYVALDFFPAAPKAEVNWSANPPELPTVPGGLQS
                     LQDSVTSLVAKLNKIPFEGIGKDAQKTLADADSLLKQLRTEVMPQARDTLAAAQTALD
                     SANGALQPNSQLQQGTTDAMRELARTAAAFRTLADYLERHPEALIRGKLEDKK"
     gene            2215..2793
                     /locus_tag="VNZ06_12545"
     CDS             2215..2793
                     /locus_tag="VNZ06_12545"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HWW21630.1"
                     /translation="MMRRSLPISMAVALAALCGCASSPMSEFYTLSPQAPQASAPAAT
                     PVTVLIGVVTVPDLVDRPQIVVRSSDNKVEINEFARWADPLKTQIPSVVRADLARLLG
                     SAFVSTRPMAGDPASAYRVQLDVQRFDGTLGDAATVDVLWSVSPPGKGSPRSGRSTVQ
                     EHCAGAGYDALVAAYSRALVTVSRDISASIRP"
     gene            2816..>3057
                     /locus_tag="VNZ06_12550"
     CDS             2816..>3057
                     /locus_tag="VNZ06_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21631.1"
                     /translation="MGPVFVDVAEQARLNAAREAQVPWKKWGPYLSERQWGTVREDYS
                     ENGDAWNYFTHDQARSRAYHWGEDGLAGISDDKQQL"
BASE COUNT          521 a          954 c         1001 g          581 t
ORIGIN      
        1 accgccctgc gttgcggcct gctgagctgc catagctgtg gcctgctggt gaagtcgacg
       61 gctcatgtgc acggcgccgc gtgtccgcgc tgcggcgccc atctgcatgc gcgcaaaccc
      121 gacagtatcg cgcgcacctg ggccttcctg attgccgcca tcgtgctgta tatcccggcg
      181 aatgcgctgc ccgtgatgaa caccagttcg ctgttcggct cggagaagga caccatcctg
      241 agcggcgtgg tctatctctg gacctcgggt tcatggccgc tcgccgcggt ggttttcatc
      301 gccagcatcg cggtgccgat gctgaagata ctggcgctga tgtacctcgt aatctccacg
      361 cagcgacgct cggtcatgct cctgcagcaa cgaacccgca tctatcggct ggtggagctg
      421 gtgggccgct ggtcgatgct cgatatctac gtcatcacca ttctggtcgc gctcgtgcag
      481 ttcaacgcgc tggccacgat tcaggccggg ccggccgcgg tcgcgttcgg ttcggtagtc
      541 gtgctgacaa tgttcgcggc catgtcgttc gacccgcgcc tgatctggga cgcgatggag
      601 gatcatcatg tctagacctc ccgtgccacc agagcttcct gaagcggtgg ccacgcctcg
      661 ctcgcgctgg cgcatgcaga tcgtctggct ggtgccgatc attgcggtgc tgatcggcgg
      721 ctggctcgcg gtcaaggcgg tcctcgaaca ggggccgacg gtcaccatca gcttcacgac
      781 cggcgaaggt ctcgaagcgg gcaagaccaa aatcaagttc aagaacgtgg atgtcggtgt
      841 ggtcaagagc gtgaccttgt cgagagacca ccgcagcgtt gtcgcgaccg ccgaactggt
      901 aaaggacgcg tcaaacatgc tggtcgacga tacgcgcttc tgggtcgtac gcccacggat
      961 ttccgggggc accgtatcgg gcatcggtac gctgctgtcc ggttccttta tcggcatgga
     1021 tctgggcgcc tcgacgaagt cgcggcgcga ctatgtcggt ctcgagacgc cgccggtttt
     1081 cgccagcggc gtgcccgggc gggagttcgt gctgaagagc gaggatatgg gctcgctcga
     1141 tatcggctcc ccggtcttct tcagacgcct gcaggtggga cagatcacct cgtattcact
     1201 cgacgcggac ggcaagggtg tcacgctgcg ggtcttcgtc aacgcgccct atgacaagta
     1261 cgtgcaggac gatacgcggt tctggcacgc aagcggcgtc gacgtgtccc tgggtaccga
     1321 tggcgtcaag gtcaacacgc aatctgtggt cgcgatcctg atcggcggcc ttgcgttcca
     1381 gacgccggat gagacgaccg gtcagtccga ggctgccgtt cgtaccgaat tcatgctgtt
     1441 ccacgatcgc gcggaggcga tgaagcgcca tgaccgcatc atcgagagct atgtcttcaa
     1501 tttcaacgac tcggtgcgcg ggttgacggt cggtgcgccg gtcgatttcc gcggcatcgt
     1561 ggtgggcgag gtggccgcga tctatacgcg tttcgatcaa gccaagaagc agttcagcat
     1621 tccggtcgag gtgcgccttt atccggagcg gttcacatcg cgttaccagt ctggcgggtc
     1681 cggaggccgg ctctcatcgg atccccaccg gatggcggaa tggctggtct cgcagggcct
     1741 gcgagcgcag ttgcgaaccg gcaacctgtt gaccggacag ttgtatgttg cgctcgattt
     1801 cttccccgct gcgcccaaag ccgaggtgaa ctggagcgcg aacccgccgg aactgccgac
     1861 cgtgcctggc ggactgcagt cactgcagga ttcggtcacc agtctggtgg ccaagctgaa
     1921 caagatcccg ttcgaaggga tcggcaagga cgcgcaaaag acgctggcgg atgcggattc
     1981 acttctcaaa cagctgcgca ccgaggtgat gccgcaggct cgggacacgc tcgccgccgc
     2041 gcagacggcg ctggactcgg cgaacggtgc gttgcagccg aactcgcagt tgcagcaggg
     2101 caccaccgat gcaatgcgtg aactcgcgcg cactgcggcg gccttccgta ccctcgcgga
     2161 ctatctcgaa cgccatccag aggcgctgat ccgcggcaag ctggaggata agaaatgatg
     2221 cgccgatcct tacccatttc catggcggtg gcgctcgccg cgttgtgcgg ttgtgcgtcg
     2281 tcgccgatgt cggagttcta tacattgagt ccccaggcgc cgcaggccag cgcccccgcc
     2341 gccactccgg tcacggtgct gatcggggtg gtgacggtgc ctgacctcgt cgaccggccg
     2401 cagatcgtgg tccgctccag cgacaacaag gtcgagatca acgaattcgc ccgctgggcg
     2461 gatcccttga aaacccagat cccgagcgtg gtccgcgccg atctcgcgcg tctgcttggc
     2521 agcgcattcg tgtccacccg cccgatggcc ggcgatccgg cctcggccta ccgggtgcag
     2581 ctcgatgtcc agcgctttga cggcacgctc ggcgatgcgg ccacggtcga cgtgttgtgg
     2641 tcggtgtcgc cgcccgggaa aggttcgccg cgcagcggac gcagcactgt gcaagagcac
     2701 tgtgcaggcg ccggttacga tgcgttggtg gctgcctata gccgggccct cgtcacggtc
     2761 agccgcgaca tctccgcttc gatccgcccg tagcccatcc gcacggagga aaagcatggg
     2821 tcccgtattc gtggacgtag cggaacaggc gcgcttgaac gccgcgcgtg aggctcaagt
     2881 cccatggaag aaatggggac cctatctcag tgagcgacag tgggggacgg tccgggagga
     2941 ctacagcgaa aacggcgatg cctggaacta cttcacccat gaccaggcgc gttcgcgcgc
     3001 ctatcactgg ggcgaggacg ggctggcggg catctcagac gacaagcagc agctttg
//
LOCUS       DATMRM010000415         6790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2101197, whole genome shotgun sequence.
ACCESSION   DATMRM010000415 DATMRM010000000
VERSION     DATMRM010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2101197"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VNZ06_12555"
     CDS             complement(<1..749)
                     /locus_tag="VNZ06_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21632.1"
                     /translation="MRTPLLFSALVVASCGLVYELVAGTLASYLLGDSVTQFSTIIGA
                     YLFAMGIGSWLSRYIDGSLVDNFVRVEIVLGLIGGFSAAILFLVFGEVSHFRVALYSL
                     VGLIGVMVGLEIPLLLRILQNEYALADLVARVLAADYVGALIASLLFPLLLVPRLGLI
                     RTSFLFGLLNVAVAWVLLLRPSQEPRTALRFAGRHRLSACAAMVLLVGGFAYADDIMA
                     VSEEGQYPGQAVFSKSTPYQRIVVTREADDIR"
     gene            complement(782..997)
                     /locus_tag="VNZ06_12560"
     CDS             complement(782..997)
                     /locus_tag="VNZ06_12560"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HWW21633.1"
                     /translation="MNFTNLGPVGNSILFAAIGLVIYGVGFWLLDRITPYHLWQEINE
                     KQNIALAVLIGSMAIGLAMIISAAIHG"
     gene            complement(1014..1169)
                     /locus_tag="VNZ06_12565"
     CDS             complement(1014..1169)
                     /locus_tag="VNZ06_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21634.1"
                     /translation="MMRQFAYVLVCGAALLYLVGANARGYVPFAGPPTRYASSYGHSG
                     GGFFFHK"
     gene            complement(1166..2446)
                     /locus_tag="VNZ06_12570"
     CDS             complement(1166..2446)
                     /locus_tag="VNZ06_12570"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HWW21635.1"
                     /translation="MAAPGSAKMIACPACGASITLRAIGQSVMIACPSCGSHIDVSRP
                     DIQLIQKYTLVTRNLHIPLGTRGTLRKQTFEVIGALGRSSGAGGRWEEYLLFNPYLGF
                     RWLVYDEGHWNLGQMIKDTSTVHPDVGVPYQGHRFRKFDMDKAVVDWVVGEFYWRVST
                     GESVLATDYIAPPLMLSCEKSGQEVTWTLLEYIEPAEVEAAFHNHSPARSYLGINQPN
                     TAAQTLRSVRRLALWAIVAAFLVQIVCAALAKSTVIPLGTYNLKHKLSDETQVYGPFS
                     LPRAHSVDEVVASGNIDNAWVELQGTLTNTDTGQSYRFVNAFQYYHGYDSDGSWSEGS
                     RNGGALIPNVPAGTYTLLVDGTSGDNSGRPLDTSIQIGLRYDVVVWRNFWIAVLLILS
                     YPIYLAFRSGVAERERWSDSDYSPTGANSQRVRR"
     gene            complement(2446..3087)
                     /locus_tag="VNZ06_12575"
     CDS             complement(2446..3087)
                     /locus_tag="VNZ06_12575"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HWW21636.1"
                     /translation="MGSPTCPSCGAPVQFRSAAPYSVCPYCRSLLVRRDACLEAIGRV
                     AEVPDDLSPLQLHTAGVFEKGHFTLVGRIRKTWEQGSWSEWCANFDSPQFGWLAEAQG
                     DLVMTFEHPMKELGGIASVEALKTAPPGTTVNIAGRAYSVSDIKVVTVTAAEGDLTSA
                     AITGMNMTSIDLRGPGVAFATIELAAQQSRLYVGRYVEFDECKFSNLRQLEGW"
     gene            complement(3180..4079)
                     /locus_tag="VNZ06_12580"
     CDS             complement(3180..4079)
                     /locus_tag="VNZ06_12580"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HWW21637.1"
                     /translation="MADYRVPAFDQGKLIRYVGYGVLVILGLVFIWPFYTVPTGSRGV
                     VTTFGAITGIEGEGLAVLPPWRKLTIFSIRAEQANVDNAEGSTYDQQPVKVSLTVRYS
                     IATDRVSEVFEKYSHNGDLSSYVETATQEIFKAVTARYTAPDLIAQRAKVSGDINQAL
                     SEKLTKYGAQVINIDMRNFSFSDSYMRAINEKVTQEQLRLAAENKLKTVEAEQKQKVA
                     VAEAEASAQRARADGEAYSQLKIAEAQAEAIKVQNSALAQGGNVLELRRIEVERTKAD
                     KWDGKLPEAIYAGAPIPFLNVSK"
     gene            complement(4201..5271)
                     /locus_tag="VNZ06_12585"
     CDS             complement(4201..5271)
                     /locus_tag="VNZ06_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWW21638.1"
                     /translation="MSDVKPIDVALLRRFSPFEGMKKDNLDALARKVVVNKLAANRVL
                     FKEGDREKRSYWLVAGLLELREGERTIAMIRAGSPEARNPIAPQLPRKVTARAVDPIE
                     YLVIDSELLDMMITWDQTGTYEVAELQAHMGTNPGDDWMTTLLQTQAFQRIPPANIQA
                     IFMRMQRISCRAGETIIQQGSEGDFFYAIVSGKCLVTRETPMNRGGLKLAELSVGDTF
                     GEEALISEAKRNATVAMLTDGVLMRLNKADFRALMNEPLVQWINYPQAKEMVVKGAKW
                     LDVRLPSEYQNLAIEGATNIPLYFIRLKISTLNRQTKYVTYCDTGRRSSAAAFILMER
                     GFDAYVLRGGLDSAERDVRRAS"
     gene            complement(5353..>6790)
                     /locus_tag="VNZ06_12590"
     CDS             complement(5353..>6790)
                     /locus_tag="VNZ06_12590"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HWW21639.1"
                     /translation="FIRSTPPRHRPPLAERVLSWPMVRPFIRPALLRQVARSARREHY
                     PAPYAIVDLWSKYGAQGEAAFTAEAHSIASLFHSDSARNLIRVFLLQDRLKGLGVGKG
                     GLAIERIHVIGAGVMGGDIAAWCALRGLNVTLQDREMKFVEPALERAQALFAKRLNGP
                     ERAAAAGRLQADVSGAGVASADIVIEAIFEDLSAKQALYAVAEPRMRTAAVLASNTSS
                     LTLESLATTLHDQGHLVGLHFFNPVPQMPLVEVVHYPATRPEVLSGAMAFARRIDKLP
                     LPCRSSPGFLVNRVLFPYLHEAFHAAGEGVDFNAIDRAATDFGMPVGPIELSDVVGLD
                     VVLHVGEIVTRELKQEPPAFVSTVRDLVAARKLGRKTGQGFYTWQEGKVVRSPGVGAA
                     PEDLADRLILSVVNECVACLREQVVEDADLVDAGVIFGTGFAPFRGGPLTYARARGID
                     QCLARLKSFEQRYGERFRPDPGWAAINA"
BASE COUNT         1283 a         2126 c         2143 g         1238 t
ORIGIN      
        1 cgaatgtcat ccgcctcgcg tgtgacgacg atgcgctgat agggcgtcga cttggagaac
       61 accgcctgac cgggatactg accctcttcg ctcaccgcca tgatgtcatc ggcgtaagcg
      121 aaaccgccca cgagcagcac cattgccgcg cacgccgaga ggcgatgccg acccgcaaag
      181 cgcagggcgg ttctcggttc ctgactgggt cgcaacagga gaacccaggc aaccgccaca
      241 ttgagcaggc cgaacagaaa actggtacgg atcaggccga gacgcggcac caacagcagc
      301 gggaacagga gcgaggcgat caacgccccg acatagtcgg cggccagtac acgtgccacc
      361 aggtcggcga gcgcatattc gttctgtagg atgcgcagca gtagcggaat ctcaaggcct
      421 accatgaccc cgatcaaccc gaccagcgaa tacagagcca cgcggaaatg cgaaacctcg
      481 ccgaacacca gaaacaggat cgctgccgaa aagccgccga tcagtcccag gacgatctcg
      541 acccgcacga agttgtcgac cagcgagccg tcgatgtagc gcgagagcca ggaaccgatg
      601 cccatcgcga acagatacgc accgatgatg gttgagaact gcgtgactga gtcgccgagc
      661 aggtagctgg ccagcgtgcc ggcgaccagc tcatagacca atccgcagct ggcgacgacc
      721 agtgccgaga acagcagcgg cgtgcgcatc gagcgcatcg ttggagtgcg gctcgcgctg
      781 gctagccgtg aatcgccgcc gaaatgatca tggccaggcc gatcgccatc gaaccgatca
      841 ggaccgcgag ggcaatgttc tgcttttcgt tgatctcctg ccacaggtgg tagggagtga
      901 tgcggtcgag cagccagaat ccgacgccgt agataaccag tccgatcgcg gcgaacagga
      961 tcgagttgcc gacgggtccc aggttggtga agttcacgac agtgctcctt ggattatttg
     1021 tgaaagaaga aaccgccacc ggagtggccg tagctggagg cgtaacgtgt gggcgggccg
     1081 gcgaacggaa cgtagccgcg cgcgttggcc ccgaccagat acagcagcgc cgccccgcat
     1141 accaggacat aggcgaattg cctcatcatc gacggaccct ctgggagttg gcgccggtgg
     1201 gactgtagtc gctgtctgac cagcgctcgc gttcggccac gccgctgcgg aatgcgagat
     1261 agatcggata gctcaggatc agcagcaccg cgatccagaa gttgcgccat accaccacgt
     1321 cgtagcgcag cccgatctga atgctggtgt cgagcgggcg gccggagttg tcaccgctgg
     1381 tcccgtccac gagcagggtg tacgtgccgg ccggcacatt cgggatcaag gctccgccat
     1441 tgcgcgagcc ttcgctccag gaaccatcgc tgtcgtagcc gtggtagtac tgaaacgcat
     1501 tgacgaaacg gtagctctga ccggtgtcgg tgttggtgag cgttccctgc agttcgaccc
     1561 aggcgttgtc gatgttgccg gaggcgacca cttcatcgac cgaatgcgcg cggggcaacg
     1621 agaaaggtcc atagacctgc gtctcgtcgc tgagcttgtg cttgaggttg taggtgccca
     1681 acggtatcac cgtgctcttt gccagcgcgg cgcacacgat ctgaaccagg aacgccgcga
     1741 caatcgccca caaggccagt ctgcgcaccg accgcagtgt ctgcgcagcg gtgttcggtt
     1801 gattgatgcc cagatagctg cgagcggggc tgtggttgtg aaacgcggct tcaacctccg
     1861 cgggttcgat gtactcgagc agggtccagg tgacctcctg cccggatttc tcgcacgaga
     1921 gcatcagtgg cggcgcaatg tagtcggtcg ccagcacact ctcgccggtg gacactcgcc
     1981 agtagaactc accaaccacc cagtcgacga ccgctttgtc catgtcgaac ttgcggaagc
     2041 ggtgtccctg atagggcacg ccgacgtcgg ggtggacggt agaggtgtcc ttgatcatct
     2101 ggcccagatt ccagtgaccc tcgtcgtaca ccagccagcg aaagccgagg taggggttga
     2161 acaacaggta ctcctcccag cgtccgccag cacccgacga gcgacccagg gcgccgatca
     2221 cctcgaaggt ctgcttacgc agcgtgccgc gtgtaccgag cgggatgtgc aggttgcggg
     2281 tgaccagcgt gtacttctgg atgagctgga tgtccggtcg ggacacgtcg atgtggctgc
     2341 cacaactcgg gcaggcaatc atcacgctct gaccgatcgc acgcagcgtg atgctggctc
     2401 cgcaggccgg gcaggcgatc atttttgcgc tgccgggagc ggccattacc aaccctccag
     2461 ctgccgcagg ttcgagaatt tgcactcgtc gaactcgacg tagcgtccga cgtacagccg
     2521 cgactgctgc gcggcgagtt caatcgtcgc gaaggcaacg ccgggacctc gcagatcgat
     2581 gctggtcatg ttcatgccgg tgatcgcggc gctggtgagg tcgccttcgg cggcggtcac
     2641 cgtgacgact ttgatgtcgg agacactgta agcccggccg gcgatgttga ccgtggtgcc
     2701 tggcggagcg gtcttgagcg cctcgacaga ggcgataccg cccagttcct tcatcgggtg
     2761 ctcgaaggtc atcaccagat cgccctgggc ttccgccagc cagccaaact gcggcgaatc
     2821 gaaattggcg caccactcgc tccagctgcc ttgttcccag gttttgcgga tacgccccac
     2881 cagggtgaaa tgccccttct cgaacacgcc agcggtatgc agctgcagcg gactcaggtc
     2941 atcgggcact tcggcgactc ggccgatggc ttcgaggcag gcgtcgcgac gcacgagcag
     3001 cgaacggcaa tacgggcaca ccgaataggg cgcagcgctc cggaattgca ccggagcgcc
     3061 acacgaaggg cacgtcggac tccccatccg cctctctccc tggccgcttt tgtgtggcag
     3121 ggaatatacc ggaagacggc gttggtgcgg gtgcggcgat cgtgcgccgg cggcggtgcc
     3181 tacttgctga cgttgaggaa ggggatcggg gctccggcgt agatcgcttc cggcagtttg
     3241 ccgtcccact tgtcagcctt ggtgcgctcg acctcgatgc ggcgcaattc cagcacattg
     3301 ccgccctggg ccagcgcgga attctgtacc ttgatcgcct cggcctgcgc ctcggcgatc
     3361 ttgagctgcg agtaggcctc gccatcggca cgcgctctct gggcgctggc ttccgcttcg
     3421 gcgaccgcga ccttctgctt ctgctcggct tcgacggtct tcagcttgtt ctccgccgcg
     3481 aggcgcagct gctcctgcgt gaccttctca ttgatggccc gcatgtacga atcggagaac
     3541 gagaagttgc gcatgtcgat gttgatgacc tgcgcgccgt acttggtgag tttctccgac
     3601 agcgcctgat tgatgtcgcc cgagaccttg gcgcgttgcg cgatcagatc cggagcggtg
     3661 tagcgcgcgg tgaccgcctt gaagatttcc tgggtcgcgg tttccacata ggaggacagg
     3721 tcaccgttgt gcgagtactt ctcgaacact tcggagacgc ggtcggtagc gatgctatag
     3781 cgcacggtca ggctcacctt caccggctgc tggtcgtagg tgctgccctc ggcattgtcc
     3841 acattggcct gttcggcgcg gatgctgaaa atcgtcagct tgcgccatgg cggcaggacc
     3901 gccagtccct cgccttcgat gccggtaatg gcgccaaagg tggtgaccac gccacggctg
     3961 cccgtgggga cggtataaaa cggccagatg aacaccaatc ctaagatgac cagcacgcca
     4021 taaccgacgt agcggatgag cttgccctga tcgaaagccg ggacacgata gtccgccata
     4081 gaccctccca ccttgggctt tagcgccctt atcaccccaa tcttgggggt ggggtcggtt
     4141 atttcaagga gtcgttgtag gcgtggagcg ccgagcgcaa gtcaggctgc aatcgctcgt
     4201 ctaggaggcg cgccgaacat cgcgctcggc actgtccaga ccaccgcgca gcacgtaggc
     4261 gtcgaagccg cgttccatca ggatgaacgc tgccgcggag ctgcgccgtc cggtgtcgca
     4321 gtaggtgacg tatttggtct ggcgattgag cgtcgagatc ttgagacgaa tgaaatacag
     4381 cggaatattg gtggcgcctt cgatcgcgag attctggtac tcggacggca gacgcacgtc
     4441 gagccatttc gcgcccttga cgaccatttc cttggcctgc ggatagttga tccactgcac
     4501 cagcggctcg ttcatcagcg ctcggaaatc ggccttattg aggcgcatca gcacgccgtc
     4561 ggtcagcatc gcgacggtgg cattgcgctt ggcctcggag atcagcgctt cctcaccgaa
     4621 ggtgtcaccg acgctgagct cggcgagctt caggccgcca cgattcatcg gtgtctcgcg
     4681 cgtgacgagg cacttgccgc tgacgatcgc gtagaagaaa tcgccctcgg agccttgctg
     4741 gatgatggtc tcgccggcgc gacacgagat gcgctgcatg cgcatgaaaa tcgcctggat
     4801 gttggcgggc gggatgcgct ggaatgcctg ggtctgcagc agcgtggtca tccagtcatc
     4861 gccaggattg gtacccatgt gcgcctgcag ctcggcgacc tcataggtgc cggtctgatc
     4921 ccaggtgatc atcatgtcga gcagctcgct gtcgatcacg aggtactcga tcggatcgac
     4981 cgcgcgggcg gtgaccttgc gcggcagctg gggcgcgatg gggttgcgcg cttcgggact
     5041 gcccgcgcga atcattgcga tcgtgcgttc gccttcgcgc agttcgagta gaccggcaac
     5101 cagccagtac gagcgcttct cgcgatcgcc ttccttgaac aggacgcgat tcgcggcgag
     5161 cttgttgacg acgactttac gggccagcgc gtcgagattg tctttcttca ttccctcgaa
     5221 tggcgagaat cttcgaagca gcgccacatc gatgggcttc acgtcgctca tgcgcgagcc
     5281 tcagttcctc atatgattca agttaacaaa tcgatggtag cacagcccct ttgctgctcc
     5341 cctgacgcca gttcacgcat ttattgcggc ccaacccgga tccggtcgga agcgctcgcc
     5401 ataacgttgt tcgaatgact taagtcgggc gagacattga tcaatgccgc gggcccgagc
     5461 gtaggtgagc gggccgccgc ggaacggtgc gaaaccagta ccgaagatca cgccagcgtc
     5521 gaccaggtcg gcgtcctcga cgacctgttc acgcaggcaa gcgacgcatt cattgacgac
     5581 cgacagtatc agacgatccg ccaggtcctc cggtgccgcc ccgacgcctg gcgaacgcac
     5641 cactttgcct tcctgccagg tatagaaacc ctgacccgtc ttgcggccaa gtttcctggc
     5701 cgcgaccaga tcgcgcaccg tgctgacgaa agccggcggc tcctgcttca gttcgcgcgt
     5761 cacgatctcc ccgacgtgca atacgacgtc aaggcctacc acgtcggaca gttcaatcgg
     5821 tcccacgggc atgccgaaat ccgtggccgc gcgatcaatc gcgttgaaat ccacaccttc
     5881 ccccgccgcg tggaaagcct cgtgaaggta agggaacagc acgcgattca ccagaaatcc
     5941 ggggctgctg cggcacggaa gcggcagttt gtcgatgcgt cgcgcgaacg ccatcgcgcc
     6001 gctgagcacc tcgggacgag tggccgggta gtgcacgacc tccaccagcg gcatctgcgg
     6061 taccgggttg aagaagtgca gtccgaccag gtgtccctga tcgtgcaggg ttgtggcgag
     6121 cgattcgagc gtcagactcg aggtattgct ggcgagtacc gctgcggtgc gcatgcgcgg
     6181 ttccgccacc gcatacagcg cctgcttggc ggacaggtcc tcgaagatcg cctcgatcac
     6241 gatgtccgca ctggcaacgc ccgcgccaga gacgtccgct tgcagacgac ccgcggcggc
     6301 ggcacgctcc gggccgttca gtcgcttggc gaacagcgcc tgggcgcgct cgagtgccgg
     6361 ctcgacaaac ttcatctcgc ggtcctgcaa cgtcacgttg aggccacgca gcgcgcacca
     6421 ggccgcgata tcccccccca tgacaccggc gccgatcacg tgaatgcgct caatcgctag
     6481 gccgcctttg cctactccca agcccttcaa tcggtcctgc agcaggaaga cgcgaatcag
     6541 gttgcgggcg ctgtcgctgt gaaacagcga agcaatcgag tgagcctcgg ccgtgaaggc
     6601 ggcctcgcct tgcgccccgt acttcgacca caggtccacg atggcatacg gggcggggta
     6661 gtgctcgcgg cgcgcgctac gggcgacctg gcgcagcagt gcggggcgaa tgaacggccg
     6721 caccatcggc cagcttaaga cgcgctccgc caacggcggc cggtgccgcg gcggtgtgct
     6781 acggatgaaa
//
LOCUS       DATMRM010000416         6711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3322137, whole genome shotgun sequence.
ACCESSION   DATMRM010000416 DATMRM010000000
VERSION     DATMRM010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3322137"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1849
                     /locus_tag="VNZ06_12595"
     CDS             <1..1849
                     /locus_tag="VNZ06_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWW21640.1"
                     /translation="AEQVSRGEFRQALVSKSGDKLGELEHAFENMRLALRNTTFSRNY
                     LHSVLNSMTDAVFVTAPGGVVRIANEAACRLTGYPDGELIGMDLINLLDQPEKLGTDP
                     LSIAREAGESVLRTRTGQTIPVSFVGSSIATEDPQFQGEIYVARDITERKRAERRIRY
                     LARYDALTKIPNRMQFQHLLQQGIARSRRSGTGVALLYVDMDRFKEVNDTFGHASGDR
                     VLEVLTERLTRVLNTEAVLGRLAGDEFALFVDSVNENAEEAGAQVAQLARTVLHAAGE
                     AFHINQQEVFLTVSIGIALCPRDAENVIDLIRNADAAMYYSKQNGGNTYAFYSPEMNA
                     AAVERLMLKSKLRRAVERDEFVVRYQPKIDLRDGRVVGAEALLRWRLPGHGDIPPTQF
                     IPLAEENNLISAIGEWVLERVCMDFRALRRHVPDPGRISLNLSLKQLKQASFILNCRT
                     VFERHAVPTRNLELEITESTLMADPRRTVEMLNQLHDMGLHLSIDDFGTGYSSLSALQ
                     QFPIGTLKIDQSFVRDAAEDPADAVLVRTIIDMGHSLGMEVVAEGVESTRQLEFLRDS
                     RCNFAQGQLFGPARSIEELLHLLQVQQNGRSEFASLIPSTPASARPISA"
     gene            1866..3386
                     /locus_tag="VNZ06_12600"
     CDS             1866..3386
                     /locus_tag="VNZ06_12600"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWW21641.1"
                     /translation="MRAFTLRWLLRLIALTLLVAWIGSTLWHSDRRLPPGIHIGGSWE
                     NMPVSQIRFLRDLSAADASGAPLSERQIDAELQRMIEGAREIIVIDAGLFGDLPAAGP
                     KAPRLRVAPTVAAALTDTLLKAKQDQPALQVLMLVDPASSELSSASGPIDRLRAAGID
                     LVPVATGRLRSPNAAFVAFWQLCCGWWSHGKGAGSWPNPIGVGPPGVAMGLWGRIPPY
                     QRAHRQLIVADDGNGNLDGMIFSRPLNAEAALHSATALRVSGPALDATLESEFAVAQF
                     SGWFGGGAMQARAQRMLERQRQSSSGPPPPETARARVVSESMIGESVVSLIDATGRHD
                     SIEVAALYLCERDLVRALLDAARRGVAVRLLLDPDKDGYGYDRSGLPNRVVASELVAS
                     SDGAVRVRWYRTHGEQFSAGVVLIRSSRRSLLAVGTADLSRRDLDDYNLAADFIVEVA
                     PGSAPASDALNWFDTLWFNRASGGIEYSSDADVYADASQLRYWQYRLLEATGAAFD"
     gene            3459..4493
                     /locus_tag="VNZ06_12605"
     CDS             3459..4493
                     /locus_tag="VNZ06_12605"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWW21642.1"
                     /translation="MRYKDNREQSAELLRMVLPLMARHSAGFHPLSYAVWYEYVSGTN
                     PALHAAVDARLAQGAKLGDRDIEALFDAHVAMRDLDTSARMRSEIARLVKHVEGVAVE
                     AGQEVHHYGEELDGYRERLLVDSGKESIVEVVESLIDETMRVRVKTEIYHEHLKKSSE
                     EVERLRGELEMVQGLAQTDPLTGLLNRRGFEQQRQRLCGNSAAGFTVLTLDIDHFKAI
                     NDAHGHLLGDRVIVAVANVLRSCVGERGPIARQGGEEFAVLLPHTSSAGGVELAERIR
                     ASVEHGKIRRADTEETIGNVTVSIGVASCGENEQFEAMLARADGALYQSKTSGRNRVT
                     VAERVFRETA"
     gene            4564..5562
                     /gene="gnd"
                     /locus_tag="VNZ06_12610"
     CDS             4564..5562
                     /gene="gnd"
                     /locus_tag="VNZ06_12610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HWW21643.1"
                     /translation="MKIGMIGLGRMGSNMVRRLMKAGHSCVVFDNTAAAVTALAGEGA
                     TASSSTADLSSKLSSPRVVWMMVPAAGVDPLIQALLPHLGRGDILIDGGNSHYADDIR
                     REQELAARGIRYLDIGTSGGLWGLERGYCLMIGGEDQAVQYLDPVLAALAPGPSQSGI
                     GSGTASRGYLHCGSAGAGHFVKMVHNGIEYGLMAAYAEGFNLLHAANKPAQAHEASAE
                     HAPQRGVLPKTREFKMDQIAELWRHGSVVSSWLLDLLAQQLDKNPNLEDFAGHVADSG
                     EGRWTIEAAIDTSVPVPVLATALFSRFSSRGEDDYANRTLSAMRLGFGGHHEIAGK"
     gene            complement(5622..>6711)
                     /locus_tag="VNZ06_12615"
     CDS             complement(5622..>6711)
                     /locus_tag="VNZ06_12615"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HWW21644.1"
                     /translation="VALTLQRDGDYRINVDLNGTTTVLVRSGATHLTNRSGEGISLRD
                     GQGVVFAADGTLDVADAHSADSFDHWCSQRDTQWQQRQRQQQTQTPVQQDLPGTDQLN
                     DSGQWNTQSEYGAVWFPAQVGAGWAPFQNGRWAWVVPWGWTWIDKAPWGFAPFHYGRW
                     VKFNGRWGWVPPPAQAHRSFAPALIAAPASVTSTPPLIAHATHVNAPPAQILHRPVVT
                     TRDPVSAAATAMPHVVVNAPASRPLNSYQPIPSPIGPTIYARDVRPVEHSAPADNTTP
                     RDSAVHAPWVTSPARASSLTPTPPSAPRTTVSRATPIPGTAPMPATPPAPPAQPLHRI
                     APAPQPHTTPPRPSQSSAPARAAARPSS"
BASE COUNT         1196 a         2027 c         2231 g         1257 t
ORIGIN      
        1 tgccgagcag gtgagccggg gtgagtttcg ccaggcattg gtgagcaaga gcggcgataa
       61 gctcggcgaa ctcgagcacg cgttcgagaa catgcgcctg gcgctgcgca ataccacctt
      121 ttcacgcaac tacctgcaca gcgtgctcaa cagcatgacg gatgcggtgt ttgtcaccgc
      181 accaggcggg gtggtgcgta tcgcgaacga ggcggcgtgc cgcctgacgg gctacccgga
      241 cggcgagctg atcggaatgg atctgatcaa cctgctcgat cagcccgaga agctcggcac
      301 cgatcctctg tctatcgcgc gcgaagccgg cgagtcggtg cttcgtaccc ggaccggaca
      361 gacgatcccg gtttctttcg tcggctcgag tatcgccacc gaagacccgc agtttcaggg
      421 cgaaatctat gtcgcgcgcg acatcaccga acgcaagcgc gccgaacgcc gtattcgtta
      481 tctggcgcgt tacgatgcac tgaccaagat tcccaaccgc atgcagttcc agcacctgct
      541 gcagcagggc attgcccgct cgcgccgcag cggtaccggt gtggccctgc tgtatgtgga
      601 catggaccgc ttcaaggagg tcaacgatac cttcggccac gcgtccggtg atcgtgtgct
      661 cgaagtgctg accgaacgtt tgacgcgggt tctgaacacc gaggccgtgc tcggccgtct
      721 ggccggtgac gagttcgcgc tgttcgtgga tagcgtcaac gagaacgccg aagaagccgg
      781 cgcccaggtc gcgcagctcg cgcgcacggt gctgcatgcg gccggagagg cgttccatat
      841 caatcagcag gaagtgttcc tgaccgtcag catcggcatc gcgctgtgcc cgcgtgacgc
      901 ggaaaacgtc attgatctga tccgcaatgc cgatgcggcg atgtactact cgaagcagaa
      961 cggcggcaac acgtacgcct tctactcgcc ggagatgaat gcggccgcgg tcgagcggct
     1021 gatgctcaag agcaagttgc gccgcgcggt ggagcgcgat gagttcgtgg tgcgctatca
     1081 gccaaaaata gatttacgcg acggccgcgt ggtcggcgcc gaagcgctgc tgcgctggcg
     1141 attgccgggg cacggcgaca ttccgccgac gcagttcatc ccgctggctg aagagaacaa
     1201 cctgatcagc gcgatcggag aatgggtgct ggagcgcgtg tgcatggact ttcgagcgct
     1261 gcgccgtcat gtgccggatc cggggcgcat ttcgctcaac ctgtcgctga aacagctgaa
     1321 gcaggccagt ttcattctta actgccgcac cgtgttcgag cgacacgccg tgccgacgcg
     1381 gaacctggaa ttggagatta ccgaatcgac attgatggcc gatccacgcc gcacggtcga
     1441 gatgctcaac cagctgcacg acatgggcct gcatctgtcg atcgatgact tcggtaccgg
     1501 ctactcctcg ctgtcggcgc tgcaacagtt cccgatcggt acgctcaaga tcgatcagtc
     1561 gttcgtgcgc gatgccgctg aagatcccgc tgatgccgta ctggtacgca cgattatcga
     1621 catgggccat agcctgggga tggaggtggt cgcggaagga gtcgagtcca cgcgtcaact
     1681 ggagttcctg cgcgacagtc gttgcaattt tgcacagggt cagctgtttg gtccggcgcg
     1741 cagcatcgaa gagctgttgc acctgttgca ggtacagcag aacggacgct cggagttcgc
     1801 cagcctgata ccctccacac ccgcctcagc gcgcccgata tccgcctgac gggcacaatg
     1861 cccgtatgcg cgcgttcacg ctacgctggc tgttgcggct gattgctctg acgttgctgg
     1921 tcgcctggat cggcagcacg ttgtggcaca gcgaccgccg cttgccgcca ggcattcaca
     1981 tcggcggatc ctgggaaaat atgccggtca gccagatacg atttctgcgc gatctgtccg
     2041 cggccgatgc cagtggtgcg ccactgagtg agcggcagat cgacgctgag ctgcagcgca
     2101 tgatcgaggg agcgcgcgaa atcattgtga tcgatgcggg cctgttcggc gatctgccag
     2161 cggcgggccc caaagcgcct cgtctgcgtg tcgccccgac ggttgcggcg gcgctcaccg
     2221 acacgctgct gaaggccaag caggatcaac ctgcgctgca ggtactcatg ttggtcgacc
     2281 cagcgagcag cgagctgagt tcagcttccg gtccgatcga tcgactgcgt gccgccggca
     2341 tcgacctcgt gccggtcgca accggccgac tgcgatcccc caacgccgcg ttcgtggcgt
     2401 tctggcagct gtgctgtggt tggtggagtc acggcaaagg cgccggcagc tggccgaatc
     2461 cgatcggcgt cggaccgccc ggtgtggcga tgggcctgtg gggtcgcatt ccgccctatc
     2521 agcgcgctca tcgccagttg atcgtcgctg acgatggtaa cggcaatctg gacggaatga
     2581 tcttctcccg accgctgaac gccgaggcgg cactgcactc ggcgaccgcg ctgcgtgtgt
     2641 cgggtccggc gctggacgcc acgctggaat ccgagttcgc agtcgcgcag ttttccggct
     2701 ggttcggcgg tggcgccatg caggcgcgcg cgcagcgcat gctcgagcgg caacgccaga
     2761 gttcttccgg ccccccgcct ccggaaaccg ctcgcgcccg ggtcgtgagc gagtccatga
     2821 tcggtgaatc agtggtatcg ctgatcgatg ccacgggtcg gcacgacagc atcgaggtgg
     2881 cggcactcta tttgtgtgag cgcgatctgg tacgtgcact gctcgatgcg gcgcgtcggg
     2941 gtgtcgcggt acgtctgctg ctcgatccgg acaaagatgg ctacggttac gatcgctcgg
     3001 gactacccaa tcgtgtggtg gcctccgaac tggtggccag cagtgacggc gcggtgcggg
     3061 tgcgttggta tcgcacccac ggcgagcagt tcagcgcggg agttgtcctc atccgcagca
     3121 gccggcgaag cctgctggcc gtgggaaccg ctgacctcag ccggcgcgac ctggacgact
     3181 acaacctggc tgctgatttc atcgtcgagg tagcacccgg ctcagcgcct gcctccgacg
     3241 ccctgaactg gttcgatacg ctctggttca accgggcatc cggtgggatc gagtacagca
     3301 gtgatgccga cgtctatgcg gatgcctcgc aattgcgcta ctggcagtac cggctgctgg
     3361 aggccactgg cgctgcgttc gactgagacg cactcgacat ccaacgcccg ggatttatgg
     3421 ccataatcaa gcaattaagg gcagggagca tcggcttgat gcgctacaag gacaaccgtg
     3481 agcaatccgc agaattgctc aggatggtgt tgccgctgat ggcgcgccat tcagccggtt
     3541 tccacccgct aagctatgcg gtctggtacg aatacgtttc cgggaccaac ccggcgttgc
     3601 acgccgcggt cgatgcacgt ctggcgcaag gcgccaaatt gggtgaccgg gacatcgaag
     3661 cgctgtttga cgctcatgtt gcgatgcgcg acctggatac ctccgcccgc atgcgcagcg
     3721 agattgctcg cctggtcaaa catgtcgagg gcgtcgccgt cgaggccggt caggaagtgc
     3781 accactacgg ggaagagttg gatggctatc gtgagcgcct gctggtggat tccggcaagg
     3841 aatcaatcgt cgaggtggtg gaatcgttga tcgacgagac catgcgtgtg cgcgtcaaga
     3901 ccgagatcta ccacgagcat ctgaagaaaa gctccgaaga ggtcgagcgc ctgcgtggcg
     3961 aactggagat ggtacaggga ctggcgcaga ccgacccgct gaccggactg ctgaatcgtc
     4021 gtggattcga gcagcagcga cagcgcctgt gcggcaacag tgccgccggg ttcacagtgc
     4081 tgacccttga tattgatcat ttcaaggcca tcaacgatgc gcacggtcat ctgctgggcg
     4141 atcgcgtgat cgtcgcggtg gccaatgtgc tgcgcagttg cgtcggtgag cgcggaccga
     4201 ttgcccggca gggtggcgag gagttcgcgg tgctgttgcc gcacacatcc agcgctggcg
     4261 gcgtcgaact ggccgagcgc attcgtgcat cggtcgaaca cggcaagatc cgtcgtgccg
     4321 ataccgagga aaccatcggt aacgtaacgg tttcgatcgg tgtcgcaagc tgcggcgaga
     4381 acgaacaatt cgaagcgatg ttggcccgag ccgacggcgc gctctatcag tccaaaacct
     4441 ccggccgcaa tcgggtgacg gtggccgagc gcgttttccg agaaacagcc tagtcccgat
     4501 ttcacccgtc gcagttttgt gctctgatgc gcgtcggttc caccgcatca ggataggtgc
     4561 tgcatgaaaa tcggaatgat cggactgggt cggatgggct ccaacatggt ccgccgactc
     4621 atgaaagcgg gtcacagctg tgttgtattc gacaataccg ccgctgcggt gacggctctc
     4681 gctggcgaag gtgcgaccgc ttcttcatcg actgcagatc tgagttccaa actatcctct
     4741 ccgcgtgtcg tgtggatgat ggtgcctgca gccggtgtcg atcctctgat ccaagcgctg
     4801 ctgccgcatc tcggacgcgg cgacatcctg atcgacggtg gcaactcgca ttacgccgat
     4861 gacattcggc gcgagcagga gctcgccgcg cgtggcatcc gttatctgga cattgggacc
     4921 agcggcggac tgtggggtct ggaacgcggg tattgtctga tgattggtgg cgaggatcag
     4981 gctgtgcagt atctcgatcc ggtcctggct gcgctggcgc cgggaccttc gcagtccggc
     5041 atcgggagcg ggacggcaag tcgcggttac ctgcactgcg gctcggccgg ggccggtcat
     5101 ttcgtcaaga tggtccacaa cggcatcgaa tacggtctga tggccgccta cgccgaaggc
     5161 ttcaatctgc tgcatgcggc gaataagccc gcccaggcac acgaggccag cgccgagcac
     5221 gctccgcagc gtggcgtgtt gccgaagacg cgtgagttca agatggatca gatcgccgaa
     5281 ctgtggcgtc acggcagtgt ggtcagctcc tggctgctgg atctgctggc gcaacagctc
     5341 gacaagaatc caaacctgga agacttcgcc ggtcacgtcg cggattccgg cgaaggccgg
     5401 tggacgatcg aggccgcgat cgatacctcg gtgccggtac cggtgctggc aaccgcgttg
     5461 ttctcgcgtt tcagttcgcg tggcgaggat gactacgcga atcgaaccct gtcagcgatg
     5521 cgcttggggt tcggcgggca tcacgaaatc gccggcaaat agaagtgacc tgacgcgcca
     5581 gcgtggatgc aatcgaggcg gcctgaaggc attcagacgc ttcagctgct cgggcgtgca
     5641 gccgctcttg ccggcgcgct cgactgcgag gggcgaggcg gggtcgtgtg cggctgtggc
     5701 gcgggcgcga tccggtgcag cggctgggct ggcggcgcag gcggtgttgc gggcatcggc
     5761 gcggtccccg ggataggagt cgctcgggat acggttgtcc tgggtgcgga aggcggcgtc
     5821 ggcgtgagcg atgaagcgcg cgccggtgat gtcacccacg gggcgtgcac tgcgctgtcg
     5881 cgcggcgtcg tattgtcagc cggtgctgaa tgctccaccg ggcgcacgtc gcgcgcgtag
     5941 atcgtcggtc cgatcggcga cgggatcggt tggtacgagt tcaacgggcg tgacgcaggc
     6001 gcattgacca ccacgtgcgg catcgcagtc gcggccgctg acaccggatc gcgtgtcgtc
     6061 acgaccggac gatgcaggat ctgcgccgga ggagcattca catgagtggc atgcgcgatg
     6121 agcggtggtg tggacgtcac ggacgcgggc gcggcgatca gggccggcgc gaaactgcga
     6181 tgagcctgcg caggcggcgg cacccagccc cagcgaccat tgaacttgac ccagcgacca
     6241 tagtgaaacg gcgcgaatcc ccacggcgct ttgtcaatcc aggtccagcc ccacgggact
     6301 acccatgccc agcgcccgtt ttgaaaggga gcccatcccg cacccacctg agccgggaac
     6361 cacacagcgc cgtattcgga ctgagtgttc cattgtcctg agtcattgag ctggtcggtg
     6421 cccggaaggt cctgctgaac cggcgtctgc gtctgctgtt gccgttgcct ctgctgccac
     6481 tgcgtgtcgc gttgcgagca ccagtggtcg aaactgtccg cgctgtgcgc atcagcgacg
     6541 tcaagtgtgc cgtcagcggc gaacaccacg ccctgtccat cgcgcagact gataccctcg
     6601 cccgagcggt tggtgagatg tgtggcgccg ctacgcacca gcaccgtggt cgtcccgttc
     6661 agatccacat tgatccgata gtcgccatcg cgctgaagcg tcagcgcgac t
//
LOCUS       DATMRM010000417        11336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_224058, whole genome shotgun sequence.
ACCESSION   DATMRM010000417 DATMRM010000000
VERSION     DATMRM010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11336
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224058"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VNZ06_12620"
     CDS             complement(<1..876)
                     /locus_tag="VNZ06_12620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW21645.1"
                     /translation="MRRVRRMMTLRMLVLLSGILLYAMNALLAATVAPSAIREFGGIA
                     YITWLTAAYLASSIAAASAGGVLTARIGARQAFIAAGVVFGAGALTCASATGMAQMIV
                     GRFIQGAGGGLLSSLSYVLVRSSLPQSQWARAFAAISGMWGVAVLFGPLIGGLFANAG
                     SWRNAFLAVAVAAIALAITAARALEHDRPVPQPGRAAFPIGQLSLMGFSIAAICVAQV
                     CEHSALKGALILLAVMALCMVLRHNRRAGALFPTDAFSLRSALGVALWMALLLSIAND
                     PFSIYGPLFLQELHGL"
     gene            complement(922..1194)
                     /locus_tag="VNZ06_12625"
     CDS             complement(922..1194)
                     /locus_tag="VNZ06_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21646.1"
                     /translation="MKKRKKAAEPKKSTSKSAFLKAVRNAQQRAAEAKRAARVAKLQW
                     KAARSAYKEARDAAHAAKKAVRAATKALPGALRESRKAADKAKRRK"
     gene            1339..2256
                     /locus_tag="VNZ06_12630"
     CDS             1339..2256
                     /locus_tag="VNZ06_12630"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWW21647.1"
                     /translation="MISWQAMTLNGLLRLTFKRRGKKPIDLGRLRRDPVRFALAVPAG
                     FVVETLRTEDGLDFDVVASRGAPGSTAGVVVLYLHGGGYIFGSPKTHRQALIAMAKAC
                     GAPVFGLDYRLAPEHPFPAAVDDAARAYQWLLRQYPASEIVLAGDSAGAGLAIATALG
                     VRDQGLKQAAAIVGFSPYSDLAVTGDSVEANARSCAMFTPQGVREAAAMYLAGANPRD
                     PRASPLYADLRGLPPMLLFASRHELLRDDTLRLAERARAAGVKVELVVRDRLPHVWPV
                     FVALLPEAREAFATVATFARQIAAPRAQA"
     gene            2253..3788
                     /locus_tag="VNZ06_12635"
     CDS             2253..3788
                     /locus_tag="VNZ06_12635"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWW21648.1"
                     /translation="MSVLREHFDVLIIGAGLSGVGAAVTLRRDCPKRTYAILEARDSM
                     GGTWDLFRYPGIRSDSDMYTLGYSFRPWKDAKSLADGASILKYIRDTASESRIDQHIR
                     FQHCVTRASWSSDEALWTVAVERGAQRQQVEFTCSFLFTCTGYYSYAEGYSPLFPGAG
                     RFTGTVIHPQKWPTSLDYAGKRVIVIGSGATAVTLIPAMAKSAAHVTMLQRSPTYIVS
                     APNRDGVVNFLRDHLPETVAYRLVRWRNVLFGAAFYQLCRRYPLAIKNFILSGVRAAL
                     GPDFDVATHFTPRYNPWDQRMCLVPDGDLFDAIKAGSASVCTGEIETFTEHGIRLKSG
                     QQLKADIVVTATGLNVVVLGNMQLLVDGQTIDPAKTLSYKGCMYSGIPNLASTFGYTN
                     ASWTLKCELTCNYVCRVLNYMERSGRDMCLPQNLDPSVQPETFLNLTSGYIQRAAEKL
                     PMQGSKRPWKLYQNYFLDLLTFRFGAVDDGVLKFDTRRTPAASEVRHARKQLTLAAAR
                     SAH"
     gene            complement(3785..3976)
                     /locus_tag="VNZ06_12640"
     CDS             complement(3785..3976)
                     /locus_tag="VNZ06_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21649.1"
                     /translation="MRIVLTIIGVILAFFGIVWILQGLNILRQGFMAGDIKWTVIGGI
                     VLMIAIVILVTTNRRKKSA"
     gene            complement(4084..5250)
                     /locus_tag="VNZ06_12645"
     CDS             complement(4084..5250)
                     /locus_tag="VNZ06_12645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HWW21650.1"
                     /translation="MIRYSPAVRRVALGCALSIAPLIAAAQNYTQTNLVSDISGMAVS
                     TDANLKNPWGLARGAGSPFWPTNEGTGTAVLYTGSGGIVPLVVTIPNATGVSGPSQPT
                     GIIFNGTADFALSVDSSPALFMFATKEGTIAGWNQGLTPLNEAVTVVDESKQGSVFLG
                     LTWVEVEGQHFLLAANFAGRRIEAFNTHFASVPLDAFQFFDPFIPRDFAPYNVQAVGN
                     NVVVTYARQNATKTAADDSNCSDGCGVVDVFTKRGIFVQRLQGGKWLQSPWGVALAPQ
                     DFGFFSHDLLIGNRASGTIAAFNPVDGRFIGNLEDTNSDTLVISGLWAIEFDNRAPNE
                     FGTTTTALPVIGGSTTLSSTGPALFFVAGINNYADGLYGALTPTAPELNAEDHE"
     gene            complement(5570..8584)
                     /locus_tag="VNZ06_12650"
     CDS             complement(5570..8584)
                     /locus_tag="VNZ06_12650"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWW21651.1"
                     /translation="MREPALIRTLERRLRRETQGEVLCDPASRGRYATDASIYQIMPL
                     GVFIPRADSDVSAAIDIARELGIPILPRGAGSSQCGQTVGEALVIDVSRHLRHVLSID
                     ATARTALVQPGVVLDDLNAQLKAHGLWFPVDVSTGAQATLGGMAGNNSCGSRSIAYGN
                     MVHNVLGLSAWLSDGSAVDFGPVPALQGRASQIASFVARLASEHRAQIIERWPKLLRR
                     VGGYNLDIFENQSERPYSRDGHVNLAHLLVGSEGTLALTRTLRLQLRELPRRTVLAVL
                     SFPSFRAAMQAPQHLVRLKPSAVELIDRIMLELSAANPVYRRLIEPVLMRQPAAVLLV
                     EFSGEDPQRLLRQLEELSTVMADFGLEGEVIRLTEPEAQRRVWEVRKAGLNIMMSLKG
                     DGKPVSFIEDCAVPLQHLADYTDALTEVFARHGTHGTWYAHASVGTLHVRPILDMRRD
                     GEGGGAVRMRRIAQEASELVRRYQGAYSGEHGDGLCRGEWIRWQFGPALYEALRAIKQ
                     HIDPIGLFNPGRIIDPPRMDDVALLRFAPPSAPRPYRTQALKAALDWSVWDVQNDPMT
                     QTLTAPGTGGDNTGGFAKAVEMCNNNGHCRKFDAGAMCPSYRVTRDEQHVTRGRANTL
                     RLALSGQLGPGGLASEAVHEAMDLCVSCKACRRECPTGVDMARMKIEFLSHYTAQHGL
                     TLKDTLIARLPDYAHALSRIPWLPNLRERVPGARWLGERLLGLSATRTLPRWRRDTYW
                     RQQRLIPTVSRDEALQRAASDRKVAVLFVDTFTGLFETENAFAAARVLTTAGYSIHVL
                     RRAGGHYCCGRTYLSCGQVSAAKSRLQELISAAEPFARAGIAVVGLEPSCLLTLRDEA
                     LVLRLGPAAHTVASQAMLFEEFVAREQRSGRLTLRLKSTSTPVLVHGHCHQKAFAAVD
                     AMLEVLRLVPGANPTLIQSSCCGMAGSFGYEAAHQEISRQMAEASLLPAVRAVPDAVV
                     IADGTSCRQQIWEGTARRALHSARLIDQLSEG"
     gene            8646..9893
                     /locus_tag="VNZ06_12655"
     CDS             8646..9893
                     /locus_tag="VNZ06_12655"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWW21652.1"
                     /translation="MLQLDAHASGHHWLQVPGPGPVPDRILRAISLPTIDHRGPEFGQ
                     LGINVLTAVKTLFRTTQPVIIYCASGTGAWEAALSNTLSPGDHVLMYDTGHFATLWSA
                     LATRLGLSVELLGWQGADPELPSVPSWRRGVHAELIEQRLRRDDAHTIRAVCVVHNET
                     STGTLSDIAAVRRALDGARHPALLMVDTISSLGCTDYRHDEWGVDVTISGSQKGLMLP
                     PGLSFNAISKRALHAMKSARLPRCYWSWDEMLESNRNGYWPHTPNTNLLYGLSEALEM
                     LRAEGLERVFARHQRWGACVRAAVQAWGLPIACIEPERYSPVLTAVITPQEVDAEALR
                     RLIYQRFDLSLGTGLGKLKGRTFRIGHLGASNDLTVLATLVACEMGLKEAGIRLKSSG
                     VQVAMHYLAQHPAPVPILKSAAQ"
     gene            complement(9903..>11336)
                     /locus_tag="VNZ06_12660"
     CDS             complement(9903..>11336)
                     /locus_tag="VNZ06_12660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HWW21653.1"
                     /translation="FPVALAFDSVAAGVVFGLVPVMWIVFNALVLYNIAVTSGRFDAF
                     CDWLLANMPNDRRVALVVIGFCFGCLMEGISGFGTPVAITSALLILIGMPPLEALTFT
                     LIFNTAPVAFGALGAPITTLGQVTDLPAVKLGAMVGRQLPFIAFILPFYVMGIYGGMK
                     SMRALWPMLFVAGGSFALAQFFCSNYVDYSLTDVLSAIISLAVTLIFVRLWSPSPDPE
                     YAVRRPAPSAPSGQTRAPVAGWQGWIPWLVVSAVVIIWTREKIYLIGQQVIHWPGLHN
                     AVYITLYKKQYAANWNFQPLGTGTAILVAAIITSALVGVGPGRFIKVLADTWRQSRLA
                     ILTVALIVGLAYLMNYSGMNYTLGLGVSSAGIFFPLLSAFLGWVAVFLSGSDTSGNAL
                     FGNLQVVAAHQLNLNPLLIAATNSSGGVMGKMISPQNIATGVSVTNLKGQEGVVLART
                     FKHSIILTLLLGLLVLAQQYLMPWMVP"
BASE COUNT         2163 a         3624 c         3494 g         2055 t
ORIGIN      
        1 caggccatgc agctcctgca ggaacagcgg accgtagatc gagaacggat cgttcgcgat
       61 cgacagcaac agagccatcc aaagagcgac gcccagcgca ctgcgcagcg agaaggcatc
      121 tgtcggaaac agcgcgcctg cgcgccggtt gtggcgcagc accatgcaga gtgccatcac
      181 ggccagcagg atcaacgcac ccttcagagc gctatgctcg cagacctgtg cgacgcatat
      241 cgcagcgatg gaaaagccca tcaacgagag ctgcccaatg ggaaacgccg cccgccccgg
      301 ctgaggtacg ggacgatcgt gttcgagtgc gcgcgccgca gtgatggcaa gtgctatcgc
      361 tgccaccgcc accgccagga atgcgtttcg ccacgaaccg gcgttggcga acaatccgcc
      421 gatgagcggc ccgaacagca ccgcgacgcc ccacataccg gagatcgccg caaacgcgcg
      481 cgcccactga ctctgcggta gtgagcttcg caccagcaca taggagagcg acgacagcag
      541 gccgccgccg gctccctgga tgaagcggcc gacgatcatt tgcgccatgc cagtagcgct
      601 cgcgcaggtg agtgcaccgg cgccaaagac gacaccggca gcgatgaatg cctgccgcgc
      661 gccgatgcgt gccgtcagca cgcccccggc cgaggccgcg gcgatcgacg aggccagata
      721 cgccgccgtg agccaggtga tgtacgcgat gccaccgaac tcacgtattg cactgggagc
      781 gacggtcgca gcgagcaacg cgttcatcgc gtacagcagt atcccgctca gcagcacgag
      841 cattcgcagc gtcatcatgc ggcgcacacg acgcacgacc cggcgtcgtt ttgtttcaga
      901 tttgaggcag tttcttgaac gctatttgcg tcgtttggcc ttgtcggcgg ccttgcggct
      961 ttcgcgcaat gcccccggca acgccttggt cgccgcgcgc actgctttct tcgccgcatg
     1021 agcggcatca cgcgcttctt tgtaggccga ccttgcggct ttccactgca gcttcgccac
     1081 gcgtgccgcg cgcttggctt cggccgcccg ctgctgcgcg ttgcgcaccg ctttcaaaaa
     1141 tgcgcttttc gaagtggatt tcttcggttc cgccgctttt tttcgctttt tcataatgaa
     1201 gatcggacat tgaacctgcg ctacggattg acattttaat tgactctcgc tccagccgct
     1261 aattttgcgc gtttcgtgcc tgagtactct agttcgctcc ggcgggaatt gtgagatcga
     1321 tactgtaata tggcgttcat gatcagttgg caggccatga cgctcaacgg ccttctgcgc
     1381 ctgacgttca agcgccgggg caagaagccg atcgacctgg ggcgactgcg ccgggatccg
     1441 gtacggtttg cgctcgcggt tccggccggt ttcgtcgtcg aaacactgcg caccgaggac
     1501 gggctcgatt ttgacgtggt tgcctcccgt ggcgcgcccg gctcgaccgc cggcgtcgtc
     1561 gtactgtacc tgcacggcgg cggatacatc ttcggctcgc cgaagacgca ccgtcaggcg
     1621 ctgatcgcga tggccaaagc ttgcggtgcg ccggtctttg gcctcgacta tcgactggcg
     1681 ccggaacatc cgttccctgc cgcagttgac gacgcggctc gagcctacca atggctgctg
     1741 cggcaatacc cggcgagtga gatcgtactg gccggagatt cagccggagc cggactggca
     1801 attgcaaccg cgctgggtgt gcgggaccag ggactcaaac aggccgccgc gatcgtaggt
     1861 ttctcgccgt attcggatct tgccgtgacc ggtgactcgg tcgaagccaa tgctcgcagc
     1921 tgcgcgatgt ttacgccgca gggcgtgcgc gaagccgcgg cgatgtacct ggccggtgcc
     1981 aacccgcgcg acccgcgcgc ctcaccgttg tatgcggact tgcgcggctt accgccgatg
     2041 ctgctgtttg ccagccgcca cgagctcctg cgtgatgaca cactgcggct ggcggaacgc
     2101 gccagggctg ccggtgtcaa agtggaactg gtggtgcgtg atcggctgcc gcacgtctgg
     2161 cccgttttcg ttgcattgtt gccggaagct cgcgaggcgt tcgcaaccgt tgccacattt
     2221 gctcggcaga ttgccgcgcc gcgcgcgcag gcgtgagcgt gttacgcgaa cactttgatg
     2281 tcctgatcat cggcgccggt ctgtccggcg tgggtgcggc ggtcacactg cggcgcgact
     2341 gcccgaagag gacctacgcg atcctggaag cgcgcgacag catgggcggc acctgggatc
     2401 tgtttcgcta tccggggatt cgttctgatt cggacatgta tacgctcggg tattccttcc
     2461 gcccgtggaa ggatgccaag tctctggccg acggtgcctc gattctgaag tacatcaggg
     2521 acaccgccag cgaaagccgc attgatcaac acatccgctt ccagcactgc gtgacacgcg
     2581 cgtcatggtc atccgacgag gcgctgtgga cggtcgcagt ggagcgcgga gcgcagaggc
     2641 aacaggtcga attcacctgc agctttctgt ttacctgcac cggctactac agctacgcag
     2701 agggctattc gccgctgttc cccggcgcgg ggcgctttac cgggacggtc attcacccac
     2761 agaaatggcc gacgtcgctc gactacgccg gcaagcgcgt gatcgtcatc ggcagcgggg
     2821 ccacggctgt gacgctgatt ccggcgatgg cgaagagcgc cgctcacgtc acaatgctgc
     2881 agcgatcgcc cacgtatatc gtgtcggcgc cgaaccgcga tggcgtggtg aattttcttc
     2941 gcgaccatct gccggaaacg gtggcttacc gactggtgcg ttggcgcaat gtgctgttcg
     3001 gcgccgcctt ctaccagcta tgccggcgct atcctctcgc gatcaagaat ttcatcctca
     3061 gcggagtgcg cgcagcgctc gggccggact tcgacgtcgc cacgcacttc acgccccgat
     3121 acaacccctg ggatcagcgc atgtgtctgg taccggatgg cgacctgttc gacgccatca
     3181 aggcgggcag cgcttcggtc tgcaccggag agatcgagac cttcacggaa catggcatca
     3241 ggctgaagag cgggcagcag ctcaaggcgg atatcgtcgt cacggcaacc ggtctgaacg
     3301 tcgtggtact gggaaacatg cagctgctgg tggacggtca gacgatcgat ccggccaaga
     3361 ccctcagcta caagggctgc atgtacagcg gcattcccaa tcttgcctcc acgttcggtt
     3421 acaccaacgc ctcgtggacg ctcaagtgtg agctgacctg caactacgtg tgccgagtgc
     3481 tcaactacat ggagcgcagc gggcgtgaca tgtgcctgcc gcagaatctc gatccgtcgg
     3541 tacagccgga aacgttcctg aacctgacat cgggttacat ccagcgcgcc gccgaaaagt
     3601 tgccgatgca gggatcaaag aggccgtgga agctgtatca gaactacttc ctcgatctgc
     3661 tcacttttcg cttcggcgcg gtcgacgacg gtgtgctgaa attcgacacg cgtcgtacgc
     3721 cggcggcatc tgaggttcgg catgcgcgca agcaattgac gctcgcagct gcacgctcag
     3781 ctcactaggc cgactttttc cggcgattgg tggtcacgag gatgacgatc gcgatcatca
     3841 gcacgatccc accgattacc gtccacttga tgtcgccggc catgaagccc tgtctgagga
     3901 tattcaggcc ctgcagaatc cacacgatac cgaagaaggc cagaatcaca ccgatgatag
     3961 tcagaacgat tcgcacgttg tccccctgtg taagccgcag ccttgaagcc tcaagcaaag
     4021 ggcccggccg atctgcggcg gccgggcccg tgacggacga tcaccctgta ttctagctca
     4081 aggctattcg tggtcttcgg cattcagctc cggagccgtc ggcgtcaatg ccccgtacag
     4141 accgtcggca tagttgttga tgccggccac aaagaacagc gcaggtccgg tcgagctcag
     4201 cgtggtgctg ccccctatca ccggcagtgc cgtggtggtg gtgccaaact cgttcggcgc
     4261 ccggttgtcg aactcgatgg cccatagccc gctgatgacc agcgtgtcgc tgttggtgtc
     4321 ctcaagattg ccgatgaatc tgccgtctac cgggttgaag gcggcaatcg tgccactggc
     4381 gcggttgcca atcagcaggt catggctgaa gaaaccaaag tcctgcggtg ccagcgcgac
     4441 cccccacggg gattgcagcc actttccgcc ctgcaggcgc tgcacgaaga ttccccgctt
     4501 ggtgaataca tcgacgacgc cgcaaccgtc gctgcagttc gaatcgtctg cggccgtctt
     4561 ggtggcattc tggcgcgcat aggtgaccac gacattgtta cccacggcct gcacgttgta
     4621 cggggcaaaa tcgcgtggga tgaatggatc gaagaactgg aacgcgtcca gcggcacgct
     4681 ggcaaagtgg gtgttgaacg cttcgatacg acgaccggca aaattggccg ccagcaggaa
     4741 gtgctgcccc tcgacttcca cccacgtcag accgaggaac accgaaccct gcttggattc
     4801 gtccacgacc gtgactgcct cgttgagcgg agtcagaccc tggttccagc cggcgatcgt
     4861 gccttccttg gtcgcgaaca tgaacagcgc cggtgagctg tccacgctga gtgcgaaatc
     4921 agcggtaccg ttgaagatga tgccggtcgg ttgcgaaggc ccgctgacac ctgtggcgtt
     4981 gggaatcgtt accactaacg gtacgatgcc accactgccg gtgtacagca ccgccgtacc
     5041 ggtgccttca ttggtcggcc agaacggtga gccggcgcca cgcgccagtc cccacggatt
     5101 tttcagattg gcatcggtgc tgacggccat gccagagata tcggacacca ggttggtttg
     5161 cgtgtagttc tgcgctgcag cgatcagcgg cgcgatcgac agtgcgcagc ccagagcgac
     5221 tcggcgtacc gcaggtgagt atcggatcat ttgaaggctc ctcccctaag gactgaaaac
     5281 gggattgaaa cgaacagccc tggggctgtc gcgcgctcga ctataggcgc agcaaaagcg
     5341 cgcggcacgc actataagtt tgcgaaccta tcaaactatt ccgctccccg ggtgtactca
     5401 catccttggc agcgttgata gggtctgtca cgcagctgtc attgcgaaca cagcaatcag
     5461 cattcggcga tagtttatgc atcgcgcgct gcatagccgg aaggcgatgc cgagtttgta
     5521 agaactatcg tgtcaatgct tgcgcgttga actattcagc gccgacgcat cagccctcac
     5581 tcagctgatc gatgagtcgc gcgctgtgca aggcgcgtcg cgcggtgcct tcccagatct
     5641 gctgccggca gctggtgcca tccgcgatca ccacggcatc cggcaccgcg cgcacagcgg
     5701 gcagcagtga cgcctctgcc atctgccgcg agatctcttg atgtgcggcc tcatatccga
     5761 aactgccggc catgccgcaa cacgagctct ggatgagtgt cggattcgca cccggaacca
     5821 ggcgcaggac ctccagcatc gcatccaccg cggcgaacgc cttctgatga caatgaccgt
     5881 gcaccagcac cggcgttgag gtgctcttca gtcgtaacgt gagccgcccg cttcgctgtt
     5941 cgcgtgcgac aaattcttca aacagcatgg cctgggacgc cacggtatgc gccgccgggc
     6001 ccagacgcag taccagcgcc tcatcgcgca aggtcagcag gcacgagggc tcaagtccaa
     6061 ccacggcaat gccggcacgc gcgaacggct ccgcggcact gatcagttcc tgcagccgcg
     6121 acttcgcagc gctcacctga ccgcagctca gataggttcg cccgcagcaa tagtgaccgc
     6181 cggctctcct cagaacatga atcgagtagc ccgccgtcgt gagcactcgc gccgccgcaa
     6241 acgcgttctc ggtttcgaac agaccggtaa acgtatccac gaacaacacg gcgaccttgc
     6301 ggtcagaggc cgcccgctgc agtgcctcat cgcgcgacac cgtcggaatg aggcgctgct
     6361 gccgccagta ggtgtcgcga cgccagcgcg gcagcgtgcg agttgccgac aggcccagca
     6421 aacgctcgcc cagccaacga gcaccgggca cgcgctcgcg cagattcggc agccacggga
     6481 tgcgactgag cgcgtgcgca tagtccggaa ggcgcgcgat cagagtgtct ttgagcgtga
     6541 gcccatgctg cgctgtgtag tgcgacagga actcgatctt catcctggcc atgtccacgc
     6601 cggtcgggca ttcgcgccgg caggccttgc agctcacgca cagatccatc gcctcatgca
     6661 ccgcctcgct cgccagtccg cccggcccga gctgtcccga tagcgcgagc cgcagtgtat
     6721 tggcgcggcc gcgcgtcacg tgctgctcat cgcgtgtcac gcgatagctg gggcacatcg
     6781 caccggcatc gaacttgcgg cagtggccgt tgttgttgca catctcgacg gccttggcga
     6841 agccaccggt gttgtcgccg ccagtgccag gggcggtgag ggtctgcgtc atgggatcgt
     6901 tctgcacatc ccacaccgac cagtcgagcg ccgccttcag tgcctgggtg cgatagggcc
     6961 gcggcgccga tggcggcgca aagcgcagca acgcgacatc gtccattcgc ggcgggtcga
     7021 tgatgcgccc tgggttgaac aggccgatcg gatcgatgtg ttgtttgatg gcgcgcagcg
     7081 cctcatacag cgcaggcccg aactgccagc gaatccattc gccgcggcac agtccatcgc
     7141 catgctcgcc gctgtacgcg ccctggtacc ggcgcaccag ttccgaggcc tcctgcgcga
     7201 tgcggcgcat acgcaccgcg ccgccgccct cgccgtcccg acgcatatcc agtatcggcc
     7261 gcacatgcag cgttccgacc gaagcatgcg cataccaggt gccgtgagtc ccatggcggg
     7321 cgaacacttc ggtcagtgca tcggtgtagt cggccagatg ctgcagcggt acggcacagt
     7381 cttcgataaa actgaccggc ttgccgtcac ccttgaggct catcatgatg ttgaggccgg
     7441 ctttgcgcac ttcccacacg cgccgctgcg cctcgggttc ggtcaggcgt atcacctcgc
     7501 cttccaatcc gaaatccgcc atcacagtgc tgagctcctc gagttggcgc agcagccgct
     7561 gcggatcctc gccgctgaac tccaccagca gcaccgctgc cggctgcctc atcaacaccg
     7621 gctcgatcag cctccgatag acaggattgg ccgcactgag ctcgagcatg atgcgatcga
     7681 tcagctcgac cgcactgggc ttgagtcgaa ccagatgctg tggcgcctgc atcgccgcac
     7741 gaaaactggg gaaactgagc accgcgagca ccgtcctgcg cggcagctcg cgcagctgca
     7801 acctcagcgt gcgcgtcaag gcgagcgtgc cctccgagcc caccagcaaa tgggcaagat
     7861 tcacgtggcc gtcgcggcta tacggtcgct cgctctggtt ctcaaagatg tcgaggttgt
     7921 agccgccgac gcgccgcagt agcttcggcc agcgctcgat gatttgcgct cggtgctcgc
     7981 tcgcaagcct ggcaacgaag ctcgcgatct ggctggcccg tccctggagt gccggcaccg
     8041 gtccgaaatc gacagcgctg ccatcggaaa gccaggccga taacccgaga acgttatgca
     8101 ccatgttgcc gtaggctatc gagcgcgaac cgcaggaatt gttgccggcc atgccgccga
     8161 gcgtagcctg tgcaccggtg gagacatcca ctggaaacca cagcccgtga gctttgagct
     8221 gtgcgttgag atcgtcgagc accacccccg gctgcaccag cgccgtgcgc gcggtcgcat
     8281 caatgctcag cacgtggcga aggtgcctgc tgacgtcgat gaccagcgct tcaccgaccg
     8341 tctggccgca ttggctgctg ccagcccctc gcggcagaat cgggatgccc agttcgcggg
     8401 cgatgtcgat cgccgctgac acgtcgctgt ctgcccgggg aatgaacacc ccgagaggca
     8461 tgatctggta gatcgaggcg tccgtggcgt agcggccacg ggaggccgga tcgcacaaga
     8521 cctcgccctg tgtttctcgc ctcaggcgcc gttccagggt ccggatcagt gcgggctctc
     8581 gcatgacttg gcaggcatct taaacgcttt gaatgtggtg tcagctattg cgacaattca
     8641 cgttgatgct acaactcgac gcacacgcca gcggccatca ttggctgcag gtcccgggtc
     8701 ctggcccggt gccggatcgg atcctgcggg ccatcagctt gccgacgatt gatcatcgcg
     8761 gaccggaatt cggtcagctc ggcatcaacg tactgactgc ggtcaagaca cttttccgca
     8821 ccacgcaacc ggtgatcatc tactgtgcgt ccggcaccgg tgcctgggag gcggccctca
     8881 gcaacacctt gagcccgggc gatcacgtgc tgatgtatga caccgggcac ttcgccacgc
     8941 tgtggtcggc gctggcaacc cggctcggac tgtcggttga gctgctgggc tggcagggag
     9001 ccgatcctga gttgccgagt gtgccgagct ggcgacgcgg tgtacacgcg gaactgatcg
     9061 agcaacggct gcgacgcgat gacgcgcaca cgatcagagc agtgtgtgtg gttcacaacg
     9121 aaacctccac cggcacactg tcggacatcg ctgccgtgcg gcgggcgctg gatggtgcac
     9181 gtcatccggc attgctcatg gtggacacca tttcaagcct gggttgcacc gactatcggc
     9241 acgatgagtg gggtgtggat gtcacgatca gcggctcgca gaaaggcctg atgctgccgc
     9301 ccggcctgag tttcaatgcg atctccaagc gtgcgctgca tgcgatgaag agcgcgcgcc
     9361 tgccgcgctg ctactggtcg tgggacgaga tgctcgagtc caatcgcaac ggctattggc
     9421 cccatacacc gaacaccaat ctgctctatg gattgtccga ggcgctcgag atgctgcgcg
     9481 ccgaggggct ggaaagagtg tttgctcgcc accagcgctg gggcgcctgc gtgcgcgccg
     9541 cggtccaggc ctgggggctg cccattgcgt gcatcgaacc cgagcgctat tccccggttc
     9601 tgaccgcggt catcacaccg caggaagtgg atgctgaggc gctgcgccga ttgatctacc
     9661 agcgcttcga tctgtcgctc ggcaccggac tgggaaagct caagggacgg accttccgta
     9721 tcggacatct gggcgccagc aacgatctga cggtgctggc gacgctggtc gcatgcgaga
     9781 tggggctgaa agaggcaggg attcgcctga aatccagcgg cgtgcaggtt gccatgcact
     9841 atctggcaca gcaccctgca ccggtgccga ttcttaaatc cgccgcgcag taatcacacg
     9901 cgctagggca ccatccaggg catgaggtac tgctgcgcca gtaccagcag accgagcagc
     9961 aacgtaagaa tgatgctgtg cttgaaagtg cgcgccagca cgactccttc ctgtcccttg
    10021 aggttcgtga ccgatacacc cgtggcaatg ttctgcggcg agatcatctt gcccatcaca
    10081 ccaccggagg agttggtggc tgcgatcagc agcggattga gattgagctg atgcgcggcc
    10141 acgacctgca gattgccaaa cagcgcattg cccgaggtgt cactgcctga caggaacacc
    10201 gcgacccagc caaggaacgc ggacagaagc ggaaagaaga tgccagccga cgacacgccc
    10261 agaccgagcg tgtagttcat cccggaatag ttcatcaggt atgcgaggcc gacaatcagc
    10321 gcaaccgtca ggatcgcgag acgactctgg cgccaggtgt cagccagtac cttgatgaag
    10381 cgccccgggc cgacaccgac cagcgcggac gtgatgatcg ccgcgaccag aatggcagtg
    10441 ccggtcccca gcggctggaa attccagtta gccgcgtact gcttcttgta caacgtgatg
    10501 tagaccgcgt tgtgcagtcc cggccagtga atgacttgct gtccgatgag gtaaatcttc
    10561 tcccgggtcc agatgatgac cacggccgag accaccagcc acgggatcca tccctgccag
    10621 ccggccaccg gtgcacgcgt ttggcccgag ggtgcgctcg gcgcggggcg ccgaacggca
    10681 tactccggat caggggatgg ggaccatagg cgcacgaaga tcagtgtcac ggccagtgag
    10741 atgatcgcgg acaacacatc cgtcagcgag taatccacat agttggagca gaaaaactgc
    10801 gccagcgcaa aactgccgcc cgccacgaac agcatcggcc acagtgcgcg cattgacttc
    10861 atgccgccgt agatgcccat cacatagaac ggcaggatga acgcgatgaa cggcagctga
    10921 cgccccacca tcgcgcccag tttgacggca ggcagatccg tgacctggcc gagtgtcgtg
    10981 atcggtgcac cgagtgcgcc gaaggccacc ggcgcggtat tgaagatcag cgtgaaggtc
    11041 agtgcttcca acggcggcat cccaatcagg atcagcagcg cgctggtgat ggccaccggg
    11101 gtgccaaaac cggaaatgcc ttccatcaga cagccgaagc agaagccgat cacgaccagt
    11161 gcgacgcgcc ggtcattggg catgttcgcc agcagccagt cgcagaacgc gtcgaagcgc
    11221 cccgacgtca cagcgatgtt gtagagcacc agggcgttga agacgatcca catgaccgga
    11281 accaggccaa acaccacacc ggccgcgacg ctgtcgaacg cgagggcgac gggaaa
//
LOCUS       DATMRM010000418        11587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4285392, whole genome shotgun sequence.
ACCESSION   DATMRM010000418 DATMRM010000000
VERSION     DATMRM010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11587
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4285392"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..759)
                     /locus_tag="VNZ06_12665"
     CDS             complement(172..759)
                     /locus_tag="VNZ06_12665"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWW21654.1"
                     /translation="MLMMLRNQMIRLGFAAYSWATRMKRVLRHAFQPRVGAPDHVTIA
                     VRDLDVARRFYCEVLGAEHFMTIDDETFRRFGRPPAPNKGEGSHHVSVYPGGSTRIDL
                     FLQQGGQPAAAAGHPHYAFKVPARHLLKWKARFEQRGIPSEGPLQLGSAGQASLYFND
                     PFGNHLELVCLGFAHPIPVRPAVMTSVIWAQEARG"
     gene            1021..1599
                     /locus_tag="VNZ06_12670"
     CDS             1021..1599
                     /locus_tag="VNZ06_12670"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HWW21655.1"
                     /translation="MNPRVDWYFAKNARWEKELRKLRTIALDCRLTEEVKWGCPCYTL
                     NASNIVLIHVFKQYCALLFFKGALLKDGHRVLVQQTKNVQAARQIRFTNVREVLKMQR
                     VVKAYILEAIEVQKSGLQVELRTTAEFEMPAEFKEKLKKLPALSKAFYALTPGRQRGY
                     LLHFSMAKQSKTREARVAKCVQPILHGKGLDD"
     gene            1720..2373
                     /locus_tag="VNZ06_12675"
     CDS             1720..2373
                     /locus_tag="VNZ06_12675"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWW21656.1"
                     /translation="MTPTITAFERSPDRGRGLARDMRIRWALEEVGQPYEVRLVSFTE
                     MKEPAHRRLHPFGQIPTYEDGDLALFESGAIIFHIAERHAGLLPDDANARARAITWMF
                     AALNTVEPPILDRGMAMLVERDKAWYEARLPILEDRVRVRLGELSSRLGDADWLDGAF
                     SAGDLLMVTVLRRLTGSGILEKYSNLSAYVARGEARLAYKRAFDAQLAVFTGKPPTG"
     gene            complement(2583..2658)
                     /locus_tag="VNZ06_12680"
     tRNA            complement(2583..2658)
                     /locus_tag="VNZ06_12680"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2623..2625),aa:Lys,seq:ctt)
     gene            complement(2824..3792)
                     /locus_tag="VNZ06_12685"
     CDS             complement(2824..3792)
                     /locus_tag="VNZ06_12685"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HWW21657.1"
                     /translation="MQPPQQKPLPIYLRYPHWFAYTLLAVVILISDLVTIASKYPFLR
                     YYDRALLPWEPYVLEFSSSILVLTAVPLLVAFDRRLPISWDTWRINLLRHAVATVVFA
                     AYKLAGELVLRKLGYAAFGSHYDFNMSILGAHFPARTVVATEYLPDAQAYVELLGLSY
                     AYRFIVLQLYGEAREFTKPEAGAPVESLERPQRFLVRKLGKEFLVAVDDIEHLEAQGN
                     YVNLHVRGHAYPLRSTMSAIGSKLDPTKFVRTHRSHIVNLNFLAEIHPLDAGDASLIL
                     RDGTPVPCSRNYRASLRERAGREPVSTSADRQKFSYRTDVSIQAPD"
     gene            3924..5207
                     /locus_tag="VNZ06_12690"
     CDS             3924..5207
                     /locus_tag="VNZ06_12690"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HWW21658.1"
                     /translation="MNAIIAATSNTERRLDLDWVRIIAFALLIGYHVACFYAAITPHN
                     QPLSPRTYGWLIVPMLALSPWRLLILFTVSGAATRFMADKMAPGTLYKTRSARLLPPA
                     LLACVVIVPPIAFVTVEQWFGYHGSFIAFLGRYFSADNHFCRAGQCMPLPLPNPFHLW
                     FVCYLWIYTVLLIGLLAWAPGALPALQRALESALRGWGLILWPAVYLGLARFALEGRF
                     PQTLDVIHDWYNHAVYLGGFLFGFSIAKSQAIWTALERIRARTLIGALMSYAAIISMA
                     IIVLGSDFNWGPKAAPALHSSLLPALTALVAGFEQWLWIAAAFGFARHYFSQNDGPVR
                     RYLTDAIFPFYIVHELTIVVGGYYLTKLGFDVRLEAALLIAGTALSCLVTYEIVRRVA
                     WLRPWFGLKRLPANMGWPSQLKPATAGVIVWDDTN"
     gene            complement(5284..5359)
                     /locus_tag="VNZ06_12695"
     tRNA            complement(5284..5359)
                     /locus_tag="VNZ06_12695"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5324..5326),aa:His,seq:gtg)
     gene            complement(5371..5447)
                     /locus_tag="VNZ06_12700"
     tRNA            complement(5371..5447)
                     /locus_tag="VNZ06_12700"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5411..5413),aa:Arg,seq:tct)
     gene            complement(5462..5538)
                     /locus_tag="VNZ06_12705"
     tRNA            complement(5462..5538)
                     /locus_tag="VNZ06_12705"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5502..5504),aa:Pro,seq:tgg)
     gene            5638..6525
                     /gene="folD"
                     /locus_tag="VNZ06_12710"
     CDS             5638..6525
                     /gene="folD"
                     /locus_tag="VNZ06_12710"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF008058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HWW21659.1"
                     /translation="MSGTSSPGARRIDGKAIAFKVLQQVGSAVRTLTVSGLRPPGFAV
                     VLVGSDPAARSHARRKRSACEQAGMHSVTHDLPATSSEAELLGLIDALNEDAAIDGIL
                     LQFPLPAHIRPGAVLEAIDPAKDIDGLHPYNIGRLLQRKPVLHPCTPYAVIKLIEHTG
                     VSPKGLDALVVGDSDILGRPMALELLLAGATTTICHADTRDVREHVARAELLVATAGR
                     ALMLRGDWIRPGAIVIDAGLNRLADGKLVGDVEFDGAAQRASWITPVPGSVGPMTIAM
                     LLRNTLEASQRRQGFRIDG"
     gene            complement(6509..8548)
                     /locus_tag="VNZ06_12715"
     CDS             complement(6509..8548)
                     /locus_tag="VNZ06_12715"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HWW21660.1"
                     /translation="MRGVRCVAWAMAVVGILGIGASGRTEASGTSPYLPLNLSPEIER
                     KIERVMVLAGKPVLTRPIAIAKVLAALPRARKHDPALCAQIDSYLDRYFAGSGVTNAS
                     GEVAGANHSTQTIPNERGERIDSPVDASVDLFYRPYDHLLFSAGAVAYAGTEGRITPD
                     GTMVSFGNQYAQMDIGWRDHWFSPLTDSSMLISTEAPPMPSFTLSNQVPIGSLGFEYE
                     LFWARMSSSDEILYKNRLNEGAPKLAGAHIGFSPLPGWSISGNGTWQYGGGGRPENIH
                     QFFDSLFSRTVIAVTPQGASTDSRFANRTVSVTSAYTFPTRNPFEAYVEYGARDTLHG
                     NRLRFHDTSLSAGVHFPELYKHFDLTIEASEWQNLWYSDYVWLEGLTEYGYPIGNWGA
                     DWRLSNADAVGAQSVMAQLGWTLPSGDEVDARVRTLQNQPYGVGNAVPVDYRRATMLT
                     LEYAQPRDGYTRGLQLDVGRDVYGKGYGRLAAFARFDGGNRTGDRGYADSEPDSDSDE
                     EAEARHAGLERFADVGLSGGRLQLNLGGFSTAQEGAQPATKNVISPHIGLGVRKQVAA
                     HQDLGVRVEVDDVSSNAMLGLRIIDYRYRLDRHLAVGAFFGFARYAAPTPAQGYYEGA
                     GIQWRDLLPHWDLALEARYFDHLQRDKVLASDPQNGDPVEWYTLFAPTLTLSRRF"
     gene            complement(8615..10060)
                     /gene="cysS"
                     /locus_tag="VNZ06_12720"
     CDS             complement(8615..10060)
                     /gene="cysS"
                     /locus_tag="VNZ06_12720"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HWW21661.1"
                     /translation="MLRIHNSLHGRKEDFRPIHPGRVGMYVCGVTVYDYCHVGHARCY
                     LVFDIVQSWLRYRGYDVNYVRNITDIDDRIIQRAAENHESIGVFSQRFIDAMHEDFRA
                     LGIGQPDHEPRATEHIPGIIAMITRLIELGHAYVGLDHDVLYSVSKFPEYGQLSGKRI
                     DDLRAGARVEVDLSKRDPLDFVLWKAAKRGEPCWPSPWGDGRPGWHIECSAMSHALLG
                     AHFDLHGGGMDLKFPHHENEIAQSVPVCGAFVNLWVHNGFVNIDNEKMSKSLGNFFTI
                     RDVLKHVRHPEVLRMFLLSSHYRGPINFSQVQLQQADAALTRLYTAMRELPLAAATVR
                     AHAGQTLDGSLRQRFEEALDDDFNTPEALAVLQALARELNAARVRSEPGRVESLAAEL
                     RALASLLGVLQVPAEQWFRFTAQIPLEVQSGSPRTTQLDEAQIEALIAARQAARKAGD
                     FAAADRIRAQLAAAGVLLEDKPGGVTSWKRV"
     gene            complement(10081..11469)
                     /gene="gltX"
                     /locus_tag="VNZ06_12725"
     CDS             complement(10081..11469)
                     /gene="gltX"
                     /locus_tag="VNZ06_12725"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWW21662.1"
                     /translation="MSVVTRFAPSPSGELHLGNLRTALFNYLLALKHGGHMVLRVEDT
                     DAARTREALVTALCADLAWMGLQWQEGPDRPGPHGPYRQSQRREIYDRYFEVLDRAGL
                     AYPCFCTQLELEVSRRAQQAAGRPPRYAGTCRELSLAERQAKLDRAIRPTLRFRVPTG
                     RRIEFDDLVHGPQSFLSDDIGDFIIRREDGTAAFFYSNALDDALMHITHVIRGEDHLS
                     NTPRQRLLLEALNLPAPQYGHVSLLLGQDGAPLSKRHGAASVRELRETGFSAMALCNQ
                     LFRLGHSTPNNAVLSLSQMAGEFDLKHLQRSPAHFDLAQLRHWQSEWVRGLSSEQARS
                     WLGPILSPELTVSQTEAFICAVLPNIVLAEDARLWQQIIFGEALAFEEPALRAAQEAG
                     PEFFTAAAGAVTERPDLGLLRTATGRKGAAFFMPLRAALTGRLHGPELAPLLAAMPTQ
                     NVRARLLHFAAG"
BASE COUNT         2200 a         3658 c         3546 g         2183 t
ORIGIN      
        1 ccgacagcgt gctctcttgt atctgctgca gacggcgcaa cagcagcacc tgttgcaacg
       61 tgaccgactc ctcggtcagc actgagagcg tgttgccctg aaggtgcgag atccggttga
      121 cgaactcctg cagcagctcg cccagctggt cgtcggcggc gactggagct ttcatccgcg
      181 cgcctcctga gcccagatga ccgaggtcat caccgcagga cgcaccggga tcgggtgcgc
      241 gaatccgagg cacaccaatt ccaggtgatt gccaaaagga tcgttgaaat aaagcgaggc
      301 ctgcccggca gagcccagct gcagcgggcc ctcgctcggt attccgcgct gctcgaaccg
      361 agccttccac ttcagcaggt gccgggccgg aaccttgaac gcatagtgcg gatgacccgc
      421 cgcggcggcc ggctggccgc cctgctgcag aaacagatcg atccgcgtgc taccacccgg
      481 atacaccgac acatgatgcg agccttcacc cttgttcggt gccggtggcc gaccgaagcg
      541 cctgaaggtc tcgtcatcaa tcgtcatgaa gtgttcggct ccgagcactt cgcagtagaa
      601 gcgccgggcg acgtccagat cgcgcacggc aatcgtgacg tgatcaggcg cgcccacacg
      661 aggctggaac gcatgtcgca gcacgcgctt catgcgggtg gcccagctgt atgccgcgaa
      721 gcccagccgg atcatctggt tgcgcaacat catcagcatg atcaaatctc cagtcgtagt
      781 taacctttca ttcacattga cgattattgc ggacaagtgt caagccgtga cgcttggcac
      841 tgggcgggaa caccccaatg tgtcgctgga atgcggtccc gaccgggaac cggaagcttg
      901 cattgcttgc ctcgatcgcg ccacatccgg ccgttcctgc ttacaatggt ggcctctact
      961 gtcgtcggga tccgtatggc caagtcctcg agttcttctt cgaatccgcc cagcggcgca
     1021 atgaatccac gggtcgattg gtacttcgcc aagaatgcga gatgggagaa ggagctcagg
     1081 aagctcagaa cgatcgccct cgactgtcgg ctgaccgaag aggtgaaatg gggctgcccg
     1141 tgctacacgc tgaacgcaag caacatcgtg ctgatccacg tgttcaagca gtattgcgcg
     1201 ctcctgttct tcaaaggagc gttgctgaag gacggccatc gcgttttggt tcaacagaca
     1261 aagaacgtgc aggccgcgcg ccagatccgg ttcaccaacg tgcgcgaagt gctgaagatg
     1321 caacgcgtcg tgaaggccta catccttgaa gcgattgaag tgcagaaatc cggtctgcaa
     1381 gtggaactgc ggacgaccgc ggagtttgaa atgcccgcgg agttcaagga aaagctaaag
     1441 aaactgcctg ccttgagcaa ggcgttttac gccttgacac ctggacgtca gcgcggctac
     1501 ctcctgcact tctccatggc caaacagagc aagacgcgcg aagccagagt ggcgaagtgc
     1561 gtgcagccaa tcctgcatgg aaagggattg gacgattaga tgcggaaacc actcttattc
     1621 catcagaccg tgtccctctt catacttggc gcgcagtgac ctcgacgcga gcggtagcta
     1681 cgcttgcttg aaccgcatgc aatcccccgg agccctccaa tgacccccac cattaccgcc
     1741 tttgaacgct cgcccgatcg cggcaggggc ctggcgcgtg acatgcgtat tcgctgggcg
     1801 cttgaagaag tgggtcagcc ttacgaggtt cgccttgttt cgttcactga gatgaaggaa
     1861 cccgcgcatc gtaggcttca tccgttcggg cagattccga cctatgaaga cggcgatctc
     1921 gccctgttcg agtcgggggc gatcattttc cacatcgcgg agcgtcatgc ggggctgctg
     1981 ccggacgatg cgaatgcccg ggcgcgtgcg atcacatgga tgtttgccgc gctcaacacg
     2041 gtggagccgc cgatccttga tcgcggaatg gccatgctcg tggagcgcga caaggcctgg
     2101 tatgaggcgc gcctgcctat cctcgaggat cgcgtccgcg tgcggctggg cgaactttcc
     2161 agtcggcttg gcgatgccga ctggctcgat ggtgcattca gcgccggtga tctattgatg
     2221 gtgacggtgc tgcgcaggct gaccggatcg ggaatattgg agaaatactc gaacctgtct
     2281 gcctatgtcg cccgtggcga agcgcggctc gcctacaagc gtgctttcga cgctcaattg
     2341 gcggtattta ctggcaagcc accgaccggc tgatgagttc ggcgccgcgc gacaagttgc
     2401 cacaggccaa ctcgccgacc ataaagggat gaatccacac ctcggtggat tcgagaagtt
     2461 ctcttagctt gatgttgcga ccacggagat gctcgaccca aactgaggta tcgaccagca
     2521 cgattggtta acgagctgca cgagcgccgc gagctggcgg gaaccgcagg ttccgcttga
     2581 attggtgggc cgtgtaggga tcgaacctac gaccaagaga ttaagagtct cctgctctac
     2641 cagctgagct aacggcccct gcttgtaagg ggtgggacga aaggatcaaa tcgtccacgg
     2701 cattgtcttt atcccgcggc ttggcggggt gtgagcccca ttccgcgggg gcgcgcagtc
     2761 tagcatcggt ggcgaccatt aaaaaagacc agggctggag cccgtcaggt ctgctgcacc
     2821 ccatcagtcg ggtgcctgaa tggaaacgtc cgtccgataa gagaatttct gacggtcggc
     2881 agatgtgctc accggctctc tgccggctct ctctctcaag gaagcacgat agttgcgact
     2941 gcagggaacc ggggttccat cgcgcagtat caggctggca tcgccggcgt ccagcggatg
     3001 aatctccgca agaaagttga gattcacgat atgactgcga tgggtgcgga caaatttggt
     3061 cggatcgagt ttcgatccga tcgccgacat ggtcgagcgc aagggataag cgtgaccacg
     3121 cacgtgcaag ttcacgtaat ttccctgcgc ttccaggtgt tcgatgtcat cgaccgccac
     3181 cagaaattcc ttgccgagct tgcgcaccag aaatcgttgc ggcctctcca gtgattcaac
     3241 cggtgcgccc gcctccggtt tcgtgaactc cctcgcttcg ccgtagagct gcagcacgat
     3301 gaagcggtac gcgtacgaga ggcccaggag ctcgacgtac gcctgcgcat cgggcagata
     3361 ttcagtcgcg accacggttc gcgctggaaa atgagcgcct aagatggaca tattgaagtc
     3421 gtagtggcta ccgaaagccg cgtagccgag cttgcgaagc accaactcgc cggcgagctt
     3481 gtaggcggca aacaccacgg tcgcgacggc atgcctgagg aggttgatgc gccaggtatc
     3541 ccagctgatg ggaaggcggc gatcgaatgc cacgagcagt ggcactgccg tgagaacgag
     3601 aatcgagctc gaaaactcca gaacgtaggg ctcccaaggc agaagagccc gatcgtagta
     3661 gcgcagaaaa gggtacttgg aggcgattgt gacgagatcg ctgatcaaga taaccacggc
     3721 caagagcgtg tacgcgaacc aatgcggata gcgcaggtag atcggcaggg gcttttgctg
     3781 aggtggctgc atgcggttaa ctaaattcgt cccggcatcc ctcgccctgt cacaaattgg
     3841 cgcccactgg tcacttcacc gtcgtcgcca cagtcccggc gagctaggtt tgcccaatat
     3901 caaggcaaac ctaggacctg ccgatgaacg cgattatagc cgccacctcg aataccgaac
     3961 gccggctcga tctggactgg gtccgcatca tcgccttcgc gctgctgatc gggtaccacg
     4021 tcgcctgctt ctatgcggcg atcacgcccc acaatcagcc ccttagcccg cgcacttacg
     4081 gatggctcat tgtcccgatg ctcgcgctca gcccctggcg gctgctgatc ctgttcaccg
     4141 tatccggcgc tgcgacccgc ttcatggccg acaagatggc ccctggaacc ctttacaaga
     4201 ctcgcagcgc gcgactgttg ccccctgcgc tattggcctg cgtggtcatc gtcccgccga
     4261 ttgcctttgt gaccgttgag caatggtttg gctaccacgg tagtttcatc gcctttctgg
     4321 gtcgctattt cagcgccgac aaccacttct gccgggccgg acagtgcatg ccgttgcccc
     4381 tgcccaaccc ctttcatctc tggttcgtct gctatctgtg gatttacacg gtgctgctga
     4441 ttggccttct ggcttgggcc ccgggcgcct tgccggctct gcaacgcgcc ttggagagcg
     4501 cattgcgggg ttggggcttg attctatggc ccgcagtcta tctcgggttg gctcgcttcg
     4561 cgctcgaagg ccgctttcct caaaccctgg atgtgatcca tgactggtac aaccacgccg
     4621 tctatctggg cggcttcctg ttcggatttt cgattgccaa atcgcaggcc atctggacag
     4681 cgttggagcg catccgcgca cgcacgctga tcggcgctct gatgagctac gcggccatca
     4741 tcagtatggc aatcatcgtg cttggctcag atttcaactg gggtcccaag gccgcaccgg
     4801 cgctccactc ttcgctgcta cccgcgctga ccgcactggt cgccgggttc gagcagtggc
     4861 tgtggatcgc ggccgcattc ggttttgcac gccattactt cagccagaac gatggaccgg
     4921 tgcgacgcta cctgacggat gcgatcttcc cgttctacat cgtccatgag ctgaccatcg
     4981 tggtcggcgg ctactacctc accaaactcg gattcgacgt gcggctggag gcggcgctgt
     5041 tgatcgccgg gacagcgctg agctgcctcg tcacttacga gatcgtgcgg cgcgtggcgt
     5101 ggctgcgacc ctggttcggc ctcaagagac ttccggcaaa catggggtgg ccctcgcaac
     5161 ttaaacccgc gaccgccgga gtcatagtct gggacgacac caactagagc gcctatcgca
     5221 cgaccgggtc cgcagcatcg cccggacgtc ttgtagcgcg aattttcttc agtcttcagg
     5281 gaatggggtg gacgacggga ctcgaacccg cgacagccgg aatcacaatc cgggactcta
     5341 ccaactgagc tacgtccacc gtcgaatcgt tggcgcgccc ggcaggactc gaacctgcga
     5401 ccaccggctt agaaggccgg tgctctatcc agctgagcta cgggcgcatt gccgtttgag
     5461 ctggtcgggg caacaggatt tgaacctgcg accctctgct cccaaagcag atgctctacc
     5521 aggctgagct atgccccgat gctcaagctt aaagagacgc acgccgcgcc ctcgtgtcgg
     5581 gccgcgcatg atacggaacc cctcttgagc gcgtcaatcg atgccacact atcagatatg
     5641 agtggcacct caagccccgg cgctcgccgg atcgacggca aagccattgc gttcaaggta
     5701 ttacagcagg ttggcagcgc cgttcgtacc ctcaccgtca gcggtcttcg gccgcccggg
     5761 tttgccgtcg tgctcgtggg cagcgatccg gccgcccgaa gccacgcgcg caggaaacgc
     5821 tctgcctgtg agcaggcggg catgcactcg gtcacacacg atctgccggc cacgagcagc
     5881 gaagccgagc tgctggggct gatcgacgcg ctcaatgagg acgccgccat tgacggcatt
     5941 ctgctgcagt tcccgctgcc cgctcacatc cgccccggcg cggttctcga agccatcgat
     6001 ccggccaagg acatcgacgg ccttcatccc tacaacatcg gccggctgtt acagcgcaaa
     6061 cccgtgctgc atccatgcac gccttacgca gtcatcaaac tgattgagca caccggcgtg
     6121 tcgccgaaag gactggacgc gttggtggtc ggcgactcgg acatcctcgg gcgaccgatg
     6181 gcgctcgagt tgttgctggc cggggccacg accaccattt gtcatgcgga tacgcgcgat
     6241 gtgcgtgagc acgtggcacg cgccgagctg ctggtcgcga ctgccggcag ggcgctgatg
     6301 cttcgcggtg actggatccg tcccggcgcg attgtgatcg acgcgggact gaaccgcctt
     6361 gcagacggca agctggtcgg cgacgtggaa ttcgatggcg ccgcccagcg agcgtcctgg
     6421 attacaccgg tgccgggcag cgtcgggccg atgaccattg cgatgctcct tcgcaacaca
     6481 ctcgaggcga gtcagcgcag gcagggattt agaatcgacg gctgagggtc aacgtcggcg
     6541 caaacagcgt gtaccactcg accgggtcgc cgttctgcgg gtcgctcgcc agtaccttgt
     6601 cgcgctgcag atggtcgaag tagcgagctt ccaatgccag gtcccagtgc ggcagcagat
     6661 cgcgccactg gatacccgcc ccctcgtaat agccctgggc cggggtgggc gcggcgtagc
     6721 gggcgaagcc aaagaacgca ccgaccgcca gatgtcggtc gagtcgatag cgatagtcga
     6781 tgattcgcag cccgagcatc gcgttcgaag agacgtcatc gacctcaacc cgcaccccca
     6841 gatcctggtg tgccgccacc tgcttgcgca caccaagacc gatatgcggt gatatcacgt
     6901 tcttggtggc gggctgcgca ccctcttgcg cagtgctgaa accgccgaga ttgagctgca
     6961 gcctgccgcc ggagaggccc acgtcggcga aacgctccag gccggcatgg cgagcttcgg
     7021 cttcttcgtc gctgtcgctg tcgggttcag agtcggcgta accgcggtca ccggtgcggt
     7081 tgccaccgtc gaagcgcgcg aacgcggcca gtcgtccata gcctttgccg tacacgtccc
     7141 ggccgacatc cagttgcagc ccgcgggtgt agccatcgcg cggctgcgcg tattccagcg
     7201 tcaacatcgt cgcgcggcgg tagtcgaccg gtactgcgtt accgacgcca tacggctggt
     7261 tctgcagggt gcgcacgcgc gcgtcaacct catcgcccga cggcagagtc caacccagtt
     7321 gcgccatcac cgactgcgct cccactgcgt cggcgttcga gaggcgccaa tcagcgcccc
     7381 agttgccgat cgggtagccg tattcggtca ggccctcgag ccacacatag tcgctgtacc
     7441 acaggttctg ccactccgag gcttcaatgg tgaggtcaaa gtgcttgtac agctccggaa
     7501 agtgcacgcc tgcggacagc gaggtgtcgt gaaagcgaag ccggttgcca tgcagcgtgt
     7561 cccgcgcgcc gtactcgacg tatgcctcaa acgggtttcg cgtcggaaac gtataggcgc
     7621 tggtgaccga aacggtccga ttcgcgaatc ggctgtcggt actcgcgccc tgcggtgtca
     7681 ccgcaatcac cgtacggctg aacagactgt cgaagaactg gtgaatattt tccggccgcc
     7741 cgccaccgcc gtactgccag gtgccattgc cggagatcga ccagccggga agtggcgaga
     7801 agccgatgtg cgcaccggcg agcttcggtg ccccttcgtt gaggcggttt ttgtagagga
     7861 tctcatcgct gctgctcatg cgcgcccaga acagctcgta ctcaaacccc aggctgccga
     7921 tcggcacctg attggacagc gtgaaggaag gcatcggcgg ggcttcggtg ctgatcagca
     7981 tgctgctgtc ggtcagcggc gagaaccagt gatcgcgcca gccgatatcc atctgcgcgt
     8041 actgattgcc gaagctcacc atcgtgccat ccggcgtgat gcgcccttcg gtgcccgcgt
     8101 aggcgacggc gcccgcggag aacagcaggt gatcgtaagg acggtagaaa aggtctactg
     8161 aggcgtccac cggcgaatcg atacgctcgc cgcgttcgtt cgggatcgtc tgcgtcgagt
     8221 ggttcgcgcc ggcgacttca ccgctggcgt tggtgacacc gctaccggcg aagtagcgat
     8281 ccaggtacga atcaatctgc gcgcacagcg caggatcgtg cttgcgcgcc ctgggcagcg
     8341 ctgccagcac ctttgcaatg gcgatcggtc gggtcagcac cggtttgccg gccagcacca
     8401 tcacgcgctc gatcttgcgc tcgatctcag gcgacagatt cagaggcaga tagggactgg
     8461 tgccgctcgc ctcggtccgg ccgctggcgc cgattcccag gatccctacc acggccatgg
     8521 cccatgcgac gcagcgaaca cccctcacgt cgcgatctct agttgtcgtc cgactgcgtt
     8581 ttgcatgctg cgcaaagtat agccgacggg cctttcagac ccttttccag ctggtgaccc
     8641 ctccgggttt gtcctccagc aggacccctg ctgccgccag ctgcgcgcgg atccgatcgg
     8701 ccgcggcgaa atcgcctgcc ttgcgcgccg cctgacgcgc ggcaatcagc gcctcgatct
     8761 gcgcctcatc gagctgcgtg gtgcgcgggc tacccgattg aacctcgagc ggaatctgcg
     8821 ccgtgaagcg gaaccactgc tcggccggca cctgcagcac gcctaggagc gaggccaggg
     8881 cgcgcagttc ggctgccagc gactcgacgc gcccgggttc gctgcgcaca cgggcggcat
     8941 tcagttcacg cgccagtgcc tgcaacacgg ccagagcctc cggcgtgttg aagtcatcat
     9001 ccagtgcttc ctcgaaacgt tgccgcaagg aaccatccaa cgtctggccg gcatgagcgc
     9061 gtacagtcgc agccgctaac ggcagctcgc gcatggccgt gtacagccgg gtaagtgccg
     9121 cgtcggcctg ctgcagttgc acctgactga aattgatggg cccgcggtag tgactcgaca
     9181 gcaggaacat ccgcagcact tccggatggc gcacatgctt gagcacatcg cgaatcgtga
     9241 agaaattgcc gagcgacttc gacatcttct cgttgtcgat gttgacgaag ccgttgtgca
     9301 cccagaggtt gacgaatgcg ccgcacaccg gcactgattg cgcgatttca ttctcatgat
     9361 gcggaaactt cagatccatg cctccgccgt gcagatcgaa atgggcgccc agcagagcat
     9421 ggctcatcgc cgagcactcg atgtgccagc ctggccgtcc atcgccccac ggtgatggcc
     9481 agcacggctc gccgcgcttg gccgccttcc acagcacgaa atcgagcgga tcgcgcttgc
     9541 tgagatcgac ttcgacacgc gcgccggcgc gcaggtcatc gatacgtttg ccggagagct
     9601 ggccgtactc ggggaacttg gagactgagt acagcacgtc gtggtccagg cccacgtagg
     9661 cgtgaccgag ttcgatcaga cgcgtgatca tcgcgatgat gccggggatg tgctccgtgg
     9721 cgcgtggctc gtgatcgggc tgcccgatcc cgagtgcgcg aaagtcctca tgcatcgcat
     9781 cgatgaagcg ctggctgaat acaccaatcg actcatgatt ctcggcggcg cgctgaatga
     9841 tccggtcatc gatatcggtg atgttgcgca cgtagttgac gtcgtagccg cggtaacgca
     9901 gccagctctg aacgatatcg aacacgaggt agcagcgcgc atgccccaca tgacagtagt
     9961 cgtacacggt cacgccgcag acgtacattc cgaccctacc gggatggatc ggacgaaaat
    10021 cttccttacg cccgtgcaaa gagttgtgta tgcgcagcat ccagtgacag tcgctgtggt
    10081 tcagccggct gcgaaatgca gcaggcgcgc acgaacattc tgagtgggca tcgccgccag
    10141 gagcggtgcg agttccggtc catgcaatcg tccggtcagc gccgcgcgca gcggcatgaa
    10201 gaacgccgcg cccttgcgtc cggtggcggt acgcagcagc cccaagtccg gccgctcggt
    10261 gacggcgccg gcggccgccg tgaagaactc cggccccgct tcctgagcgg cacgcagcgc
    10321 cggctcttcg aacgccaggg cttcgccgaa gatgatctgc tgccacagac gtgcatcttc
    10381 ggcgagcacg atattcggca gaaccgcgca aatgaacgct tcagtctgac tgaccgtgag
    10441 ctcgggtgag agaatcggcc ccagccagct acgggcctgc tcgctcgaca gaccccgcac
    10501 ccattcgctc tgccagtggc gcagctgcgc cagatcgaaa tgcgcgggcg agcgctgcag
    10561 atgcttgaga tcgaactcac cggccatctg cgacagcgac aacaccgcat tgttcggcgt
    10621 ggaatgcccc agacggaaca gctggttgca caacgccatg gcggaaaagc ccgtctcgcg
    10681 caattcgcgc acgctggcag cgccatgccg cttggaaagc ggcgcaccat cctggccgag
    10741 caacagcgat acgtggccgt actgcggtgc tggcagattc agcgcctcca gcagcagccg
    10801 ctgtcgcggc gtattgctca agtggtcctc accgcggatg acgtgcgtga tgtgcatcag
    10861 cgcatcgtcc agcgcgttgc tgtagaagaa tgccgcggta ccgtcctcgc ggcggatgat
    10921 gaaatcgccg atgtcatccg atagaaaact ttgcggaccg tgcaccagat catcgaactc
    10981 gatgcgccgg cccgtcggca cgcgaaagcg cagcgtcggg cggattgcgc gatcgagttt
    11041 tgcctgtcgc tcggccaagc tcagctcacg gcaggtaccg gcatagcgcg gcgggcgtcc
    11101 ggccgcctgc tgcgcgcggc gtgacacctc cagctccagc tgcgtgcaga agcacgggta
    11161 cgccagcccc gcgcgatcca acacctcgaa gtagcgatca tagatctcgc ggcgctgtga
    11221 ctgccggtag ggtccatgcg gaccgggccg gtccggtccc tcctgccact gcagacccat
    11281 ccaggccaga tcggcgcaca gcgcggtgac cagggcctcc ctggtgcgcg cggcgtcggt
    11341 atcctccacc cgcagcacca tgtgtccgcc gtgcttgagt gccagcagat agttgaacag
    11401 cgccgtgcgc aggttgccca gatgcagctc accgctggga ctgggtgcaa agcgcgtaac
    11461 gacgctcaag gtggacatcc gtacaatgta gcctaactac acgcttcggg cgatccgacc
    11521 gtttgagcac gctgttcatc tctgacctgc acctggatgc ttccgaaccg gcggctggcg
    11581 cgcactt
//
LOCUS       DATMRM010000419         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3322760, whole genome shotgun sequence.
ACCESSION   DATMRM010000419 DATMRM010000000
VERSION     DATMRM010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3322760"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VNZ06_12730"
     CDS             complement(<1..824)
                     /locus_tag="VNZ06_12730"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWW21663.1"
                     /translation="MIPHRRILNRLQSLSVGLALCALSLVSGGEALAAATSGAEPLVR
                     AVDSIGIPVTNITRSVDFYTRVLGFHQTLDRTVSGDAYEHLFGLYGMRLRMVRMQLGD
                     ESIELMQFLSPQGRPAPTDSSSNDRWFQHIAIIVSDMQRAYAVLRANDVTPASSEPQV
                     LPAWNANAAGIAAYYFFDPDGNHLELLQFPPDKGAAKWHLPTKSPFLGIDHTAIVVLN
                     TRASLAYYCDTLGFSVAGHSDNYGPEQERLNNVFGAHLRITALRAGAGPGVELLEYL"
     gene            complement(821..2323)
                     /locus_tag="VNZ06_12735"
     CDS             complement(821..2323)
                     /locus_tag="VNZ06_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWW21664.1"
                     /translation="MNAGHFDAIIVGSGAGGSAAAYRLSEAGLRVLILEKGPRLPVDG
                     STLDIHHVVRGRRFLSMEPWIGPRGEPLQPEEHFNLGGKTKWYGAALLRFSPVEFEAE
                     PDYAALGWPFSYAELEPFYDEAERLLGVRRFACEPSLKRILDGLGSAESAWQSSSLPM
                     ALMDGILMNAHEAAHFDGFASVGNFKGDADVGLLAKISHRPTVQIRTDTEVTELLPAD
                     RDSGRIGGVRLRNGDELHATSILLAAGALHSPRLLARYLTRAGLDKRLPSAHLVGRYL
                     KLHHLTALVAMSRQRQRDLLRKTMLTVHTGYPHSSVQALGFDAELIGTLIPRFVPRAI
                     ACRMADRAYGFFLQTEDGSHADNRVAEQAGPGGSTEPQRVLNYEPSRTPLSRLEHRRF
                     TRAFRRSLLRLGFSTFIQRIGLNGTAHACGTLVMGSDARHAVVNTRGAVHGIDGLYVV
                     DGSVLPRSSRVNPALTIYAWSLRVAALLTKSLQGHSRPANSALGQHEAPL"
     gene            complement(2320..>2572)
                     /locus_tag="VNZ06_12740"
     CDS             complement(2320..>2572)
                     /locus_tag="VNZ06_12740"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HWW21665.1"
                     /translation="VALYIWLEGPVSGMSMNPARTLASALPGGLWQSLWIYLTAPCLG
                     MWLAAWLYRGWASRAEVVSGCAKLCHSADVPCIHCGYQP"
BASE COUNT          527 a          824 c          784 g          437 t
ORIGIN      
        1 aggtactcga gcaattccac gcccggtcca gcgccggcgc gcaacgccgt gatgcgcaga
       61 tgcgctccga acacattgtt gagtcgctcc tgttcgggac catagttatc gctgtgtccg
      121 gcgacgctga atcccaacgt atcacaatag taggcgaggc tggcgcgggt gtttagtacc
      181 acgatcgcgg tgtgatcgat gccaaggaac ggtgacttcg ttggcaggtg ccacttggcg
      241 gcgcccttgt cgggcggaaa ctgcagtagt tccaggtgat tgccgtccgg gtcgaagaaa
      301 taatatgccg cgatgccggc ggcgttcgca ttccatgccg gcagcacctg aggctcgctc
      361 gaggctggcg tgacatcatt ggcacgcagc acggcatagg cgcgctgcat gtcactgacg
      421 atgatcgcga tgtgctggaa ccagcgatcg ttgctgctgg agtccgtagg tgccggacgg
      481 ccctgaggag acagaaactg catcagctcg atcgactcgt ctcccaactg catgcgcacc
      541 atgcgaaggc gcataccgta caggccgaac aggtgttcgt aagcatcgcc cgacacggta
      601 cgatccagtg tctggtgaaa cccgagcacg cgcgtataaa agtccacgga gcgagtgata
      661 ttcgtcaccg gaataccgat ggaatccact gcgcgcacca gcggttcagc gccggaagtg
      721 gcggcggcaa gcgcctcgcc tccactgacc aggctgagcg cgcagagtgc aaggccaacg
      781 ctcaggctct gcaaccgatt caatatcctg cgatgaggga tcatagcggg gcctcatgtt
      841 gacccagagc gctgttggca ggccgtgagt gaccttgaag cgacttggtc agtagcgcag
      901 ccacgcgcag actccaggcg taaatcgtga gggcgggatt gacccggctc gagcgcggca
      961 acacgctgcc gtcaaccacg tagagcccat caattccatg caccgcgcct ctggtattca
     1021 ccaccgcatg ccgggcatcg ctgcccatga ccaaggtgcc acaggcatgg gcggtgccat
     1081 tcagaccaat gcgctgaatg aaggttgaaa accccagacg cagcagtgag cggcgaaagg
     1141 cccgagtgaa gcgccgatgc tcgagtcgcg atagaggagt acgggatggc tcatagttca
     1201 gcacgcgttg cggctcggtg cttcccccag gcccagcttg ttcggcgacc cgattatccg
     1261 cgtgcgaacc gtcctcggtc tgcagaaaga agccgtaggc tcggtcggcc atgcgacagg
     1321 caatcgcgcg cggcacgaat ctcggaatca gggttccaat caattcggca tcaaacccca
     1381 gcgcctgcac actgctgtgc gggtagcctg tatgcaccgt cagcatggtt ttgcgcagca
     1441 ggtcccgctg ccgctgcctc gacatcgcga ccagcgccgt cagatgatgc agcttcaggt
     1501 agcggcccac taagtgggca ctcggcaggc gcttgtccag tccagcgcgc gtcaggtagc
     1561 gagcgagcag ccgcggggaa tgaagcgcac ccgccgcgag cagtatgctc gtcgcatgca
     1621 gttcatcgcc gttgcgcagt ctgacgcctc cgatgcggcc gctgtcgcga tcggccggca
     1681 gcaactcggt gacctcagtg tcggtgcgga tctgcacagt cggccgatgc gagatcttgg
     1741 ccaacagacc gacatccgca tcacctttga aatttccaac actcgcgaag ccgtcgaaat
     1801 gcgcagcctc gtgcgcattc atcaaaatgc cgtccatcag agccatcggc aacgacgaag
     1861 actgccaggc gctctcggcg ctacccaacc cgtccaggat gcgcttgagg ctaggctcgc
     1921 aggcaaatct gcgcacgccc agcagtcgtt ccgcctcgtc ataaaatggc tccagctcgg
     1981 cgtaactgaa cggccagccc agcgccgcat agtccggctc ggcttcgaac tcaactggcg
     2041 agaagcgcag cagcgcggcg ccgtaccact tggtcttccc gcccagattg aagtgttctt
     2101 ccggttgcag cggctcaccg cgtggcccga tccacggctc catgctcaga aaacgccgcc
     2161 ctcgcaccac atggtggatg tcgagcgtgc tgccatctac cggcagccgg ggacccttct
     2221 cgagaatcaa cacccgcagg ccggcctcgg atagccgata ggccgcggcg cttccgccgg
     2281 cgccagagcc aactatgatg gcgtcgaagt ggccagcgtt cacggttgat atccgcaatg
     2341 aatgcacgga acatcggccg aatggcacaa tttggcgcaa ccggacacga cttccgcacg
     2401 cgaggcccac ccgcgataga gccacgccgc gagccacatc ccgagacacg gcgcggtcag
     2461 atagatccac agcgattgcc acaacccgcc gggtaacgcc gatgccagtg tgcgcgcggg
     2521 gttcatgctc atgccggaca ccgggccctc gagccagatg tagagcgcga cc
//
LOCUS       DATMRM010000420         5938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3024039, whole genome shotgun sequence.
ACCESSION   DATMRM010000420 DATMRM010000000
VERSION     DATMRM010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3024039"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VNZ06_12745"
     CDS             <1..1062
                     /locus_tag="VNZ06_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21666.1"
                     /translation="PNYAGYDTSTLSYHPFLVAMQQADDMIDGLTASRSALAAIGKSM
                     TAQTTDNGQLFITGYSQGGYVAMATQRALQALNMTVTAGAPMSGPYALEAFVDAEFAG
                     QVSQGAPVVATLLLDSYQNSYGNLYSQPSELISAQFATGFTSLLPSTTSRSQLYSAGK
                     LPQFALFSATPPNATYASITPATQPAAFAASFAQGFGDPPLIINSYRLSYLQDMAASP
                     DGNFPNLTTGVPASSPQVAWRQELKANDLRDWTPTSPTLLCAADQDPSVYFFNTQFMQ
                     QYWMAHPSSNPSTALSVLDLDSASVAAGADGSLQTAFQAAKAAYAAAAGASAVAGAYH
                     ETLVPAFCLGAVISFFNQF"
     gene            1087..2559
                     /locus_tag="VNZ06_12750"
     CDS             1087..2559
                     /locus_tag="VNZ06_12750"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HWW21667.1"
                     /translation="MDASNDGSRDLLRGIARRAMQARGLEPDFSVEAQQQTQALQHAP
                     AMTADNARDQRALLWCSIDNDDSRDLDQLSVAEPLSGGAVRILVAIADVDAWVKRDSP
                     IDLHAQANTTSVYTIAQIFPMLPEPLSTDLSSLTEGQDRLAIVIQMTIGPDGTLSDPD
                     IYRARVRNKAKLAYDGVAAWLTGTAAAPARVSAVPGMDQQLRVQDQVAQRLRQVRHSH
                     GALELESIEARPVFDGDLLRDLRPDESNRAKQLIEDFMIAANGVTARFLATRGLPSLR
                     RVLRTPEKWDRIMMLARAQADTLPTAPSAQALNEFLLRRRAADPAGFPDLSLSVIKLL
                     GRGEYSLEMPGQSIQGHFGLAVRDYTHSTAPNRRFPDVIAQRLLKAALAGAPTPYSPE
                     QLRSLAAHCTAQEDNASKVERLVRKSAAAWLLASRGGHRFDGIITGAADKGTWVRIDS
                     PTAEGRVVRGFENLQVGQRVGVRLVHTDVQRGFIDFERAD"
     gene            2562..3335
                     /locus_tag="VNZ06_12755"
     CDS             2562..3335
                     /locus_tag="VNZ06_12755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWW21668.1"
                     /translation="MPHDTTPTPMLAYDDPEFLASDDGRALRILAEYLEPLHRFRLAS
                     IRDTIVFFGSARLTADGPLGRYYQDARELARLLTLWSQSLQAGSHRYVVCSGGGGGIM
                     EAANRGATEAGGRTIGLNISLPHEQRPNPYLTPDLTFEFHYFFMRKLWFAHLARAMIA
                     FPGGFGTLDELAEILTLAQTHKLEGPIPVILYGRSYWEEILNFQALVRHGVISREDLD
                     LFEFADDPAGALQILKRKLEPAPSAPGFAHSRTGESNSD"
     gene            complement(3332..4150)
                     /locus_tag="VNZ06_12760"
     CDS             complement(3332..4150)
                     /locus_tag="VNZ06_12760"
                     /inference="COORDINATES: protein motif:HMM:NF003738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HWW21669.1"
                     /translation="MPDSLRSFIERHERLFVLTGAGCSTESGIPDYRNADGEWKRSPP
                     VMFQDFMRQFSVRARYWARSLIGWPHFHAAKPNPVHRALVSLEQRARIGLLATQNVDR
                     LHQLAGSRDVVDLHGRLDVVRCMSCPQRLPREQLQRDLLQHNPGWALLDARAAPDGDA
                     ALEGRDFSQFQVPSCAACGGILKPDVVFFGEIVPPERVTRSMQAVLEADAMLVLGSSL
                     MVWSGYRFVQAAAEAGKPIAAVNLGVTRADELFQLKVVQPCSRALAFLLQSAAA"
     gene            complement(4143..5318)
                     /locus_tag="VNZ06_12765"
     CDS             complement(4143..5318)
                     /locus_tag="VNZ06_12765"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWW21670.1"
                     /translation="MSVAATVYLKECRESLRDRRVLLNALLLGPLLGPILFTMILRVS
                     IGMELDKADRPLPVVVVGAERAPNLIEALKQQGLVPRPPVADVDAAVRAQTIDVALRI
                     SESYATDWDAGRPAQVELIYDSSRRGNGTQVARLREMLRFYERRTGVMRLVARGLSPA
                     VGTPLVVADRDQATPQARGALLFAMLPYFLILTCFIGGIWLAIDSTAGERERQSLEPL
                     LANPVPRGQILLGKLAACCTFSFTSLTLGLIAFVGAAHFLPVQQLGMTLNIGPRDVAT
                     ILPIMIPLVLLIVITQMLVSAFAKTYREAQTYLGLLQLVPIIPSILLAVTPFNPPPWM
                     YAVPLLGQQLAITQALRGEVIAQTTLFLCTVTTLAAFGAVFFIARKIYESERLAVNA"
     gene            complement(5315..>5938)
                     /locus_tag="VNZ06_12770"
     CDS             complement(5315..>5938)
                     /locus_tag="VNZ06_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWW21671.1"
                     /translation="GKTTTLRMLYTLLKPDRGRISVDGLDAAAHALQVRQRLGVLPDA
                     RGLYKRLSARENIEYYAQLHALPVQTVRTRIEALVQALGMQEIIDRRVEGFSQGERVK
                     TAIARALVHDPRNVVLDEPSNGLDVMSTRNLREFLRHLRSEGRCVLFSTHIMQEVAAL
                     CDRIVIIAGGRVVANGTPESLREQAHEQSLEEAFVKIIGAATVGALA"
BASE COUNT         1094 a         1900 c         1906 g         1038 t
ORIGIN      
        1 ccgaactatg ccggctacga cacctcgacg ctgtcgtacc acccttttct ggtggccatg
       61 caacaggccg acgacatgat cgacggcctg accgcctcgc gctccgcgct ggctgcgatc
      121 ggaaagtcga tgacggcaca gaccaccgac aacggacagc tgttcatcac cggctattca
      181 cagggcggct atgtggcgat ggcgacgcag cgcgcgctgc aggcgctgaa tatgacggtg
      241 accgcggggg ccccgatgtc cggtccgtat gctctggagg cattcgtcga tgcggaattc
      301 gccggccagg tgagccaggg cgcgccggtc gtcgcgaccc tattgctcga tagctatcag
      361 aattcatacg gcaacctcta ttcgcagccg agtgagctca tctcagcaca gttcgcaacc
      421 ggattcacga gcctgttgcc cagtacgact tcgcgcagcc agctctacag cgcaggtaag
      481 ctgccgcagt ttgcgctctt cagtgcaacg ccacccaatg cgacctacgc atcgatcacg
      541 cctgcgacgc agccggccgc gttcgccgcg agcttcgcac agggctttgg cgacccgcct
      601 ctgatcatca acagctatcg cctgagctat ctgcaggaca tggcggccag tcccgatgga
      661 aattttccga atctgacgac cggcgtgccc gcgagctctc cacaagtggc gtggcgccag
      721 gagctcaagg ccaatgacct gcgcgattgg acacccacct cgccgaccct gttgtgcgcg
      781 gcagatcagg acccgagcgt gtacttcttc aacacgcagt tcatgcagca atactggatg
      841 gcgcacccct catccaatcc ctcgactgcg ctgtcggtgc tggacctgga ctcggctagc
      901 gtggcggcag gagcggatgg ctcgttgcag accgcatttc aggcagcaaa ggccgcgtat
      961 gcggccgcgg cgggtgccag cgccgttgca ggcgcctatc acgagacgct ggtaccagcg
     1021 ttttgtctgg gcgcggtgat ttcatttttt aatcaattct agcggacgcg cccgtatcat
     1081 gggcgcatgg acgcttccaa cgacggcagc cgggatctgc ttcgtggtat cgcgcggcgc
     1141 gcaatgcagg cgcgtggcct ggagcccgac ttctcggtcg aagcccagca gcagacgcaa
     1201 gcgctgcaac atgcacccgc gatgaccgcg gacaacgcgc gtgaccagcg cgctctgctg
     1261 tggtgttcca tcgacaatga tgattcgcgc gatctcgatc aactctcagt agcggagcct
     1321 ctgagcggcg gtgcggtcag aatcctggtc gccatcgccg acgtggacgc gtgggtcaag
     1381 cgcgactctc ctattgatct gcacgcgcaa gccaacacca cctcggtcta cacgatcgcg
     1441 cagatttttc cgatgctgcc cgaaccgttg tcgaccgatc tgagttcgct caccgagggc
     1501 caggatcggc tcgcgatcgt gatccagatg acgatcggtc cggacggaac actgtcagat
     1561 cctgatatct accgcgccag ggtgcgcaat aaggccaagc tcgcctacga cggtgtggcg
     1621 gcctggctga ccggcacggc cgcagcgccg gcacgtgtct cggcagtgcc gggcatggat
     1681 cagcagttgc gtgtccagga tcaggtcgcg cagcggctgc gccaggtgcg ccactcgcac
     1741 ggggctttgg aactggagtc gatcgaagcg cgaccggtat tcgatggcga tctgcttcgc
     1801 gatctcaggc cggacgagag caatcgcgcg aaacaattga tcgaggactt catgatcgcg
     1861 gccaatggcg tgacggcccg ctttctggcc acgcgcggac tgccctcgct gcgccgtgtg
     1921 ctgcgtacac cggagaagtg ggaccgaatc atgatgctgg cacgcgcaca ggcggatacg
     1981 ctgccgacgg cgcccagcgc ccaggcgctc aatgagtttc tgcttcgccg ccgggcggcc
     2041 gatccggctg gttttcccga cctgtcgttg tcggtgatca aactgttggg acggggcgaa
     2101 tacagtctgg agatgccggg acagtcgatt caggggcatt tcggtctcgc cgtgcgcgac
     2161 tacactcact cgaccgcgcc gaatcgacgc ttcccggacg tgattgcgca gcgactgctg
     2221 aaagcggcac tggccggcgc gcctacgccc tacagcccgg agcaactgcg ctcgctggca
     2281 gcgcactgca ccgcgcagga ggacaatgcc tccaaggtcg agcgactggt gcgaaaatcc
     2341 gccgccgcgt ggttgctggc ctcgcgcggc gggcacaggt tcgacggcat catcaccggc
     2401 gccgctgata agggcacctg ggtacgcatc gattcaccga cggcggaagg tcgggtggtg
     2461 cgtggcttcg aaaatctgca ggtcggtcaa cgtgtagggg ttcgactcgt gcataccgac
     2521 gtgcagcggg gattcatcga cttcgagcgc gcggactaac catgccccac gatacgaccc
     2581 ctacgccgat gctggcctat gatgatccgg agttcctggc cagcgatgac gggcgagcgc
     2641 tgcgcatcct ggcggagtac ctggagccgc tgcatcgttt tcgcctggcg agcattcgcg
     2701 acacgatcgt ttttttcggc tcggcgcgtc tgaccgccga cgggccgctg ggtcgttact
     2761 accaggacgc gcgcgagctg gcgcgtctgc tgacgctctg gtcgcagagt ctgcaggccg
     2821 gctcacaccg ctacgtggtt tgctcgggcg gcggcggtgg aatcatggaa gcggccaatc
     2881 gcggagcgac ggaagcgggt ggccgaacga tcggattgaa catcagtttg ccgcacgagc
     2941 agcggcccaa tccgtatctg acgccggacc tgacgttcga atttcactac ttcttcatgc
     3001 gcaaactgtg gttcgcgcat ctggcgcggg cgatgattgc gttccctggg ggattcggca
     3061 cgctcgatga gctggctgaa atcctgacgt tggcgcagac gcacaaactc gaaggtccga
     3121 ttcccgtcat tctttacggc cgcagctact gggaggagat tctcaacttt caggcgctgg
     3181 tacgacatgg cgtgatcagt cgcgaggatc tcgatctgtt cgaatttgca gacgatccgg
     3241 ccggtgcact gcagatcctg aagcgcaagc tcgagcccgc cccgtcagcg cccggattcg
     3301 ctcattcgcg caccggggaa tcaaacagcg actaggccgc tgcgctctgc agcaggaagg
     3361 cgagcgcacg gctgcagggt tgcacgacct tgagttgaaa caactcgtcc gcccgtgtga
     3421 cgcccagatt caccgcagcg atcggcttgc cggcctcggc cgcggcctgt acgaagcgat
     3481 agccggacca caccatcagc gaggagccga gcaccagcat cgcgtcggcc tcgagcaccg
     3541 cctgcatgct gcgcgtcacg cgctccggcg gcacgatctc gccgaagaac accacgtcgg
     3601 gtttgagtat gccgccgcac gcggcgcagg aagggacctg aaattgtgaa aaatcccgac
     3661 cctccagagc cgcatcgcca tccggcgcgg cgcgcgcgtc aagtaaggcc cagcctggat
     3721 tgtgctgcag cagatcccgc tgcagctgct cgcgcggtag gcgttgcgga cagctcatgc
     3781 accggaccac gtccaggcgg ccgtgcagat ccacgacgtc gcggctgcct gccagttgat
     3841 gcagtcggtc gacgttctgc gtcgcgagca gtccgatgcg agctcgctgc tcgaggctga
     3901 cgagtgcgcg atgcacgggg ttgggttttg cggcatgaaa gtgcggccaa ccgatgagac
     3961 tgcgcgccca gtagcgtgcc cggaccgaga actgacgcat gaagtcctga aacatcaccg
     4021 gcggcgagcg cttccattcg ccgtcggcgt tgcgatagtc cgggatgccg gactccgtgc
     4081 tgcagccggc ccccgtcagc acgaacagcc gctcatgccg ctcgatgaag ctgcggaggc
     4141 tgtcaggcat tgacggcgag tcgctccgac tcgtagatct tccgcgcgat gaaaaagacc
     4201 gcgccaaagg ctgcgagcgt ggtcacggtg cacaaaaaca gcgtcgtctg cgcaatgact
     4261 tcgccgcgca gcgcctgggt gatggccagt tgctggccga gcagcggcac ggcatacatc
     4321 catggcggag gattgaacgg tgtcaccgcc agcagaatcg acggaatgat cggcaccagc
     4381 tgcagcaggc ccagataggt ctgcgcttcg cgataggtct tcgcgaacgc cgataccagc
     4441 atctgcgtga tgacgatcag cagtaccagc ggaatcatga tcggaagaat cgtggcgacg
     4501 tcgcgcggtc cgatattcag cgtcattccg agctgctgta ccgggaggaa atgcgccgcg
     4561 ccgacaaatg cgatcagacc gagcgtgagg ctggtgaaac tgaaggtgca acaggcagcg
     4621 agcttgccca gcaggatctg gccacgcggc accgggttgg cgagcagcgg ttccagggac
     4681 tggcgctcgc gctcaccggc ggtcgagtcg attgccagcc agataccgcc aatgaagcag
     4741 gtcagaatca ggaagtaggg cagcatcgcg aacagcagcg cgccgcgggc ctggggtgtg
     4801 gcctgatcac gatcggcaac caccagcggt gtgcctaccg ccggcgatag gccccgggcg
     4861 acgagccgca tcacaccggt ccggcgctcg tagaacctca gcatctcgcg cagccgcgcg
     4921 acctgagtac cattgccgcg ccgcgaggaa tcatagatga gttccacctg cgccggtcgg
     4981 ccagcatccc agtcggtcgc atagctctcg ctgatgcgca gcgcgacgtc gatcgtctgc
     5041 gcccgtacgg cagcgtcgac gtcggccacg ggcggcctgg gcaccagacc ctgctgcttc
     5101 aatgcctcga tgagattggg cgcacgttca gcgccgacga ccacgaccgg caggggtcgg
     5161 tcagccttgt cgagctccat cccgatcgat acgcgcagga tcatcgtaaa aagaatcggt
     5221 ccaagcagtg gacccagcag cagcgcattg agcagcacgc gacgatcgcg caggctctcg
     5281 cggcattcct tgagatagac cgtggccgcg acactcatgc gagcgctccc acggtggcgg
     5341 cgccgatgat cttgacgaag gcttcctcca ggctctgctc gtgcgcctgc tcgcgcagcg
     5401 attccggtgt gccgttggcg accacgcggc ctccggcgat gatgacgatc cgatcacaca
     5461 gcgccgcgac ctcctgcatg atgtgggtcg agaacagcac gcaacgcccc tcggatctca
     5521 agtggcgcag gaactcgcgc agattgcgcg ttgacatgac gtccagtccg ttgctgggtt
     5581 catcgagcac cacattgcgc ggatcgtgaa ccagcgcgcg tgcgatggcg gtttttaccc
     5641 gttcaccctg ggagaagccc tcgacccgac ggtcgatgat ctcctgcatg ccgagcgcct
     5701 ggacgagagc ctcgatgcgg gtgcgcaccg tctgcacggg cagcgcgtgc agctgcgcat
     5761 agtactcgat gttctcacgc gcgctcaggc gcttgtacag tccgcgtgca tccggcagca
     5821 cgccgaggcg ctggcgcacc tgcagcgcgt gagcggcggc gtccaggcca tccacgctga
     5881 tgcggccgcg atcaggcttc agcaaggtgt agagcatgcg cagcgtggtg gtcttgcc
//
LOCUS       DATMRM010000421        11441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3024107, whole genome shotgun sequence.
ACCESSION   DATMRM010000421 DATMRM010000000
VERSION     DATMRM010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11441
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3024107"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VNZ06_12775"
     CDS             complement(<1..433)
                     /locus_tag="VNZ06_12775"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HWW21672.1"
                     /translation="MSHADLERVATAMVAKDKGLLAADESTSTIKKRFDKIGLESTEE
                     HRRAYRDMLFSTPGVAQWISGVILYDETLRQKSKEGVPFPDYLTKLGIIPGIKVDLGA
                     KPLALFPGETITEGLDGLRERMMEYYKLGARFAKWRAVIDIA"
     gene            543..1004
                     /locus_tag="VNZ06_12780"
     CDS             543..1004
                     /locus_tag="VNZ06_12780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HWW21673.1"
                     /translation="MLRISKLTDYATVILARLAAQPERRFTAAQISAETRLAAPTVSK
                     LLKQLHRHGLVLSTRGLHGGYLLARPAGEISAAQILDALEGPVAITECASSASHCCIE
                     STCLVSKSWQRVNFAVRRSLQEITLLELAGLTASPASSERHTAPLIQRGSL"
     gene            1001..2443
                     /gene="sufB"
                     /locus_tag="VNZ06_12785"
     CDS             1001..2443
                     /gene="sufB"
                     /locus_tag="VNZ06_12785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HWW21674.1"
                     /translation="MSAPDLSELVNRSYRHGFITDIDSDTLAPGLDEDVVRVISRKKA
                     EPQFLLDWRLKALRHWRDMREPHWAHLRYQPIDYDAISYYSAPKQKSDGPKSLSEVDP
                     KLLATYEKLGVPLHERARLAGVAVDAVFDSVSVATTFKERLAAAGVVFCPFSEAVQKH
                     PQLIEQYLGSVVPYSDNFFATLNSAVFTDGSFVYVPRGVRCPMELSTYFRINAAKTGQ
                     FERTLIIADVGSYVSYLEGCTAPMRDENQLHAAVVELVALEDAQIKYSTVQNWYPGDE
                     QGRGGIYNFVTKRGDCKGRNSRISWTQVETGSAITWKYPSCLLRGENSIGEFYSVATT
                     NNYQQADTGTKMIHIGRNTRSTIISKGISAGHGQNTYRGLVKIMPGATGARNFTQCDS
                     LLLGQQCGAHTFPYVEVRNPSATVEHEASTSRISEEQLFYCQQRGIGTEDAVNMIVSG
                     FCRSVFKELPMEFAVEAQNLLAVSLEGAVG"
     gene            2440..3201
                     /gene="sufC"
                     /locus_tag="VNZ06_12790"
     CDS             2440..3201
                     /gene="sufC"
                     /locus_tag="VNZ06_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HWW21675.1"
                     /translation="MSAELLKVSDLHVNVAGQAVLRGVSLSVRAGEVHAIMGPNGSGK
                     STLAHVLAGRPGYEIVCGSVTFEDRDLLALAPEERARAGLFLGFQYPVEIPGVNNAYL
                     LRAAVNAVRHERGETEFDAFEFLQVVRAKIKLMQIDESFLSRGVNEGFSGGEKKRNEV
                     LQMLVLEPKLAVLDETDSGLDIDALKVVAHGINSLRAPSRATVLVTHYQRLLDYVVPD
                     FVHVLARGRIARSGDRTLALELERRGYDWVSEQAA"
     gene            3213..4454
                     /locus_tag="VNZ06_12795"
     CDS             3213..4454
                     /locus_tag="VNZ06_12795"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HWW21676.1"
                     /translation="MAPSAVLERILHDFQSVASQLPDSVVPREGRQRAAAELTRLGWP
                     SARDEQWRYANLRAFERVAQFRSPAASSSEGLNLPDPLPGLERLVFVDGHRCLVGANA
                     VATAVAPVWPAEQRLGLLCDMFASDIAALRIHGNAAIELVFITSERAAGAAVYPRIQI
                     QLEAGSELKLVERHLGSPDRPTLVSTNASIALAHGAQLSHYRLQQCGANTLFSDTLSA
                     DVAADASYRVRQVGVGSASARTSAQVRLNGRAAAVDWQAIAVGRGEQVHDLALKVEHR
                     ARDTRTEEVFRGIAEGTAQIAFSGHIHIAEAAGGSDARQSLRGLIEGARAEIDLRPRL
                     EINTDEVRARHGATTGQLDQNLLFYLLARGIDPKTARALLKWAFLGDVLRTIDLPALR
                     SAAEQSAAGQLPDVAAIGALT"
     gene            4451..5716
                     /locus_tag="VNZ06_12800"
     CDS             4451..5716
                     /locus_tag="VNZ06_12800"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HWW21677.1"
                     /translation="MRAHSAAAQSNPVAASTFDSARVRADFPILARQIHGKPLTYLDS
                     AASSQRPRAVLRALEHYETSLHANVHRGVHSLSQWATEAYEGARETVRRFLNARSTRE
                     IVFVRGTTEAINLVANSWGRANLKSGDEILITYLEHHSNIVPWQLVCAATGARLRAAP
                     VRPNGEVDLDAFREMLNPRTRLVAVAHVSNALGTILPVHDIVRVSHARGVPVLLDGAQ
                     AVAHHRVDVQDIDCDFYAFSSHKIYGPTGIGALYGKESLLSAMPPWQGGGDMILSVTI
                     ESSTWNDLPWKFEAGTPNISGAVGLGAALQYVESLGRDAIVTHEQELLARTQEALSHL
                     PRVTLIGTAPSKVAVVSFTMEGVHPHDIGTILDSEGIAIRTGHHCAMPIIEQYGLAAT
                     ARASFGCYNNASDVERLVAGLAKVRDMFG"
     gene            5737..6180
                     /locus_tag="VNZ06_12805"
     CDS             5737..6180
                     /locus_tag="VNZ06_12805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HWW21678.1"
                     /translation="MDLKDLYREVILDHNRRPRNFGRVTSANAEAKGHNPLCGDKLDL
                     TARVQGERLEDIRFEGQGCAISTASASLMTEAVKGKTRQEIAQLFEEVHELLTDPSHV
                     PARDLGKLMALAGVAEFPARVKCASLCWHTLNAALGSASAPVSTE"
     gene            6184..6738
                     /gene="sufT"
                     /locus_tag="VNZ06_12810"
     CDS             6184..6738
                     /gene="sufT"
                     /locus_tag="VNZ06_12810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HWW21679.1"
                     /translation="MRVAENEPVVIHREVDAVMVPSGQPIKLKMGLAGYITQALGGSF
                     TLYVEGNLYRIAGDQADAIGKEVVLPPTLPPNATEEDVRELAWEQMRGCYDPEIPINI
                     VDLGLVYECKVTPNEDHTRNLSVSMTLTAPGCGMGDVLVQDVKEKLAAIPTVQAVDVQ
                     LTFDPPWSQSMMSEAARLQTGMTW"
     gene            6751..7230
                     /locus_tag="VNZ06_12815"
     CDS             6751..7230
                     /locus_tag="VNZ06_12815"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWW21680.1"
                     /translation="MKQLLLLRHAEAQPASPHIKDFDRILTERGRQEAAQAADALAAA
                     RLTIDEILVSPSRRTRETADSVMHRLGLRMPARAVAELYLAAPGVMLETLEGCQVTSS
                     TVLLIAHNPGISELARQLGGGSGGLSLRTAGLCHLSLAQDRWDDIGSERASAWQLLR"
     gene            7243..7845
                     /locus_tag="VNZ06_12820"
     CDS             7243..7845
                     /locus_tag="VNZ06_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HWW21681.1"
                     /translation="MKTRVVLFDVGGVLVELGGVDALLGWLGSGMSAEQLWTVWLRSP
                     AVRAFETGRIEPLAFAHALLSELGLTISAEVFLESFATWPTRPYPGALELVASIPPGY
                     QRALLSNSNTLHWPRVIDDMAFGSSFDHCFSSHLTGKIKPDREAFEHVVHRLGCQPSE
                     ILFLDDNQLNVDAARRVGMQAQRARGVEESRLALRQAGVL"
     gene            complement(7842..8555)
                     /locus_tag="VNZ06_12825"
     CDS             complement(7842..8555)
                     /locus_tag="VNZ06_12825"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWW21682.1"
                     /translation="MAAGLSRILAFDIGGTNLKAALIDAKGRMLTERLKTPTPEGCRP
                     KQMVAALVRLAKPFPRHDHIAIGFPGVVRDGRVVTAPHWGTEKWAGFELAKELSQRLG
                     NAPARLINDAEMQGLAVIEHKGLELVLTLGTGAGTALFRDGQIMPHLELAHHPIHEDM
                     TYNDYVGNAALKKHGKKRWNKRVRHMIEILESLLHYDHLYIGGGNATRVAFKLPDNVS
                     LVSNEAGLEGGAVLWGDAA"
     gene            complement(8555..9658)
                     /gene="tal"
                     /locus_tag="VNZ06_12830"
     CDS             complement(8555..9658)
                     /gene="tal"
                     /locus_tag="VNZ06_12830"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HWW21683.1"
                     /translation="MKATQQLHDLGQSLWIDNITRGMLTSGTLAKYIRELSVTGLTSN
                     PTIFEKAIKDSNFYDAAIAQKSAAGSSGEALFFDLAIEDLTQAADLFLPAHRATNGVD
                     GFVSLEVSPLLAYDTANTIKAAIELHGRAKRPNLYIKIPGTREGLPAIEQSIASGVPV
                     NVTLLFSADQYQAAAEAYMRGIEQRLKAGKDPKIESVASVFISRWDGSANGKVPKEMH
                     NRLGVAIARRTYKTCLDLWASPRGQALIKAGGRPQRLLLASTGTKDPAASDTLYIEAL
                     AAPNTVNTMPENTLLAFADHGKVSGVMPKDGGDAESVIAAFNKAGVSDAALAADLQRD
                     GAKAFEKSWNDLMSVLATKGAQLQRAGAGTGRR"
     gene            complement(9688..9840)
                     /locus_tag="VNZ06_12835"
     CDS             complement(9688..9840)
                     /locus_tag="VNZ06_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HWW21684.1"
                     /translation="MSLHLYLGPLAALIAGILILILPRLLNFIVAIYLILIGLYGLFG
                     GAVHLH"
     gene            complement(10283..>11441)
                     /locus_tag="VNZ06_12840"
     CDS             complement(10283..>11441)
                     /locus_tag="VNZ06_12840"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWW21685.1"
                     /translation="ILARTPNAPSGVKGISLFLVPKLLLREDGALGAANDVRCLSIEH
                     KLGIHASPTCVMQFGSDDAGSDPTRGAVGWVVGEENRGLEYMFIMMNMARFSVGVQGL
                     GLAERAYQQALGYARERRQGGVTIVQHPDVRRMLLTMKSTIEAMRALAGVVAEATDHA
                     RAHPDAQARQRAQRFMSLLIPVVKGWSTESAVTLTSLGVQIHGGMGFIEETGAAQYLR
                     DARITPIYEGTTGIQANDLVGRKILRDGGRAAFDLIASMSQLKVELSRSHHVHLSALA
                     GSLERAIQSLDQAVRFVLERGREHIHVAQAGAEPLLNLFGVVAGGWQLARGALAAERR
                     LQRRQGDLQYYETRIVTARFYGDTVLPRASGLAHAVVHGADSILGLADEQL"
BASE COUNT         2116 a         3605 c         3579 g         2141 t
ORIGIN      
        1 cggcgatgtc gatgaccgcg cgccacttgg cgaagcgcgc gccgagcttg tagtactcca
       61 tcatccgctc gcgcagacca tccaggccct cggtaatggt ttcgccgggg aacagcgcca
      121 gtggtttggc gcccagatcg accttgatgc ccggaatgat tcccagtttc gtcaggtaat
      181 cggggaacgg cacgccttct ttgctcttct ggcggagggt ttcatcataa agaatcactc
      241 cgctgatcca ctgcgccacg ccgggggttg agaacagcat gtcgcggtag gcgcggcggt
      301 gctcttcggt ggactccagc ccgatcttgt cgaagcgctt cttgatcgta ctggtgctct
      361 catcggcagc cagcaggccc ttgtccttgg ccaccatggc ggtggccacg cgttcgagat
      421 cggcgtgact catggctttg tacctcctgt tcgggatgcg gagaatacca aacgcctcaa
      481 gagcctagcg cttctcattc ggtccatata gtactaaact agtcctagtt taaggcggac
      541 ctatgctgcg tatcagcaaa ctgaccgatt acgccaccgt catcctggcg cgactggccg
      601 ctcagcccga gcggcgcttt acggctgccc agatttccgc cgaaacccgg ttggccgcac
      661 ccacggtgtc caagcttctg aagcagctgc atcggcacgg cctggtgctg tctacccgcg
      721 gtctgcatgg cggctatctg ctcgcccgcc ctgcgggaga gatcagcgca gcccagattc
      781 tcgatgcgct ggaaggtccg gtcgcgatta ccgagtgcgc cagcagtgcc agtcactgct
      841 gcatcgaaag cacctgcctg gtcagcaagt cctggcagcg cgtcaacttc gcagtgcgcc
      901 gctcgctgca ggaaatcacc ctgctggagc tggcgggcct gactgcctcg cccgcctcca
      961 gcgagcgtca cactgcaccg ttgatccagc gaggaagctt gtgagtgctc ccgatctcag
     1021 cgagctggtg aaccgcagtt accggcacgg tttcatcacc gacatcgact cggacacgct
     1081 cgcccctggg ctggacgaag atgtcgtgcg tgtgatctcg cgcaagaaag ctgagccaca
     1141 gttcctgctc gattggcgtc tgaaagccct acgtcactgg cgcgacatgc gcgagccgca
     1201 ttgggcacat ttgcgctatc agccgatcga ctacgacgcg atttcctatt actcggcgcc
     1261 caagcagaag tcggacggcc cgaaaagtct gtccgaagtg gatccgaagc tgctggccac
     1321 ctacgagaaa ctgggtgtgc cgctgcacga gcgtgcccgt ctggccggtg tcgcggtgga
     1381 cgcggtgttc gacagcgtct cggtcgccac gaccttcaag gagcgcctgg ctgcagccgg
     1441 cgtcgtgttc tgcccgttct ccgaggcggt gcagaagcat ccgcagctca tcgagcagta
     1501 tctgggctcc gtcgtgccct atagcgacaa cttcttcgcg acgctcaatt cggcggtgtt
     1561 caccgacggc tccttcgtct acgtaccgcg cggcgtgcgt tgtccgatgg aattgtccac
     1621 ctacttccgc atcaacgccg ccaagaccgg acagttcgag cgcacgctga tcattgccga
     1681 tgttggcagc tatgtgagct acctggaagg atgcactgcg ccgatgcgcg atgaaaacca
     1741 gctgcacgcg gcagtggtgg aactggtggc gctggaggat gcgcagatca aatactcaac
     1801 cgtgcagaac tggtatccag gcgatgagca gggccgcggc ggcatctaca acttcgtcac
     1861 caagcgcggc gactgcaagg gtcgcaattc gcgcatctcc tggacgcagg tggagaccgg
     1921 ctcggcgatc acctggaagt atccgagctg tctgctgcgc ggcgagaact cgatcggcga
     1981 gttctattcg gtcgcgacga cgaacaacta tcagcaggcc gataccggca ccaagatgat
     2041 ccatatcggc cgcaataccc gcagcaccat catttccaag ggcatttcgg ccggccacgg
     2101 ccagaatacc tatcgtggcc tggtgaagat catgccaggc gccaccggcg cacgtaactt
     2161 cacgcagtgc gattcgctgc tgcttggaca gcaatgcggc gcacatacct tcccgtacgt
     2221 cgaggtgcgt aaccccagcg cgacggtgga gcatgaggcc agcacctcgc gtatcagcga
     2281 agagcagctg ttctattgtc agcagcgcgg catcggcacc gaggacgcgg tcaacatgat
     2341 cgtcagcggc ttctgtcgca gcgtcttcaa ggagctgccg atggagttcg cggtcgaagc
     2401 acagaatctg ctggcggtga gtctggaagg agcggtggga tgagcgccga gttgttgaag
     2461 gtcagcgatc tgcacgtcaa tgtcgccgga caggcggtgc tgcgcggcgt gtccttgagc
     2521 gtgcgggcgg gcgaagtcca cgccatcatg gggccgaacg gttcgggcaa gagcacgctg
     2581 gcgcacgtgc tggcagggcg tccgggctat gagattgtct gcggcagcgt cacgttcgaa
     2641 gatcgcgatc tgctggcgct ggcgcccgaa gagcgcgccc gcgccggact gttcctgggc
     2701 tttcaatacc cggtagagat tcccggggtc aacaacgcct atctgctgcg cgccgccgtg
     2761 aatgcggtcc gccacgaacg cggtgagacc gaattcgatg cgttcgagtt cctgcaggtg
     2821 gtgcgcgcca agatcaaact gatgcagatc gatgagagtt tcctgagccg cggtgtcaac
     2881 gaagggttct ccggcggcga gaagaagcgc aacgaggttc tgcagatgct ggtgctggaa
     2941 ccgaagctgg cggtgctgga tgaaaccgat tccggtctgg acatcgatgc gctcaaagtc
     3001 gtcgcgcatg gcatcaattc gctgcgcgcg ccatcgcgtg ccaccgtgct ggtgacacac
     3061 tatcagcgct tgctcgatta cgttgtgccg gacttcgtgc atgtgctggc gcgtggtcgc
     3121 atcgcccgca gcggcgatcg gactctggcg ctggaactgg agcgccgcgg ttatgactgg
     3181 gtcagcgagc aggccgcctg agcttacgcg ccatggcccc gtcagccgta ctcgaacgca
     3241 tcctgcacga tttccagtcg gtcgcgtcgc agctgcccga cagtgtcgtc ccacgcgagg
     3301 gacgccaacg cgccgcggcg gaattgacac gcttaggttg gccgagtgcc cgcgatgagc
     3361 agtggcgcta cgccaatctg cgcgcgtttg aacgtgtcgc ccagtttcgc tcgcccgctg
     3421 cctcgtcctc cgaggggctg aacctgccgg atcccctgcc cggcttggaa cgcctggtgt
     3481 tcgtagacgg gcatcgctgc ctcgtcggcg ccaacgcggt cgccaccgcc gtggccccgg
     3541 tgtggccggc ggagcagcgc ctgggattgc tgtgcgacat gttcgccagc gacatcgcgg
     3601 cccttcgtat ccacggcaat gcggccatcg agctggtgtt catcaccagc gagcgcgcgg
     3661 ccggtgctgc ggtgtatccg cgaattcaga ttcagctcga ggccggtagc gagctcaagc
     3721 tggttgagcg gcatctgggc tcgcccgacc ggccaacgct ggtcagcacc aacgccagca
     3781 tcgctttggc ccacggtgcg cagctttcgc actaccgcct gcagcagtgc ggcgcgaaca
     3841 cgttgttcag cgacacgctg agcgcggacg tggcggccga tgccagctat cgcgtgcgcc
     3901 aggtcggcgt cggcagcgcg tccgcccgca ccagtgcgca agtgagactc aacgggcgcg
     3961 cggccgctgt ggattggcag gcgatcgcgg tcgggcgtgg cgagcaggta cacgatcttg
     4021 cgctcaaggt cgagcatcgc gcccgtgaca cccgcaccga agaagtgttt cgcggcatcg
     4081 cggaaggtac cgcgcagatt gcattcagcg gccacattca tatcgcggaa gccgcaggcg
     4141 gctcggatgc gcgacagtcg ttgcgcgggt tgattgaggg agcgcgcgcc gagattgatc
     4201 tgcggccgag gcttgagatc aataccgacg aggtacgcgc acggcacggc gcgaccaccg
     4261 gtcaactcga ccagaatctg ttgttctacc tgctcgcgcg cggcattgat ccgaagactg
     4321 cgcgcgcctt gctgaaatgg gcattcttag gggatgtgct gcggacgatc gatcttcccg
     4381 ccctgcgcag cgccgccgaa caaagtgccg ccggacaact gcccgacgtc gcggccatag
     4441 gagcgctgac atgagggccc actcggccgc cgcccaatcc aatccggtcg ccgcaagcac
     4501 atttgacagc gctcgtgtgc gcgcggactt cccgattctg gcgcgtcaga tccatggcaa
     4561 gccgcttacg tatctggaca gcgcggcctc gtcgcagcgt ccccgcgcgg tactgcgcgc
     4621 gctcgaacat tacgagactt cgctgcatgc caatgtgcat cgcggcgtcc actcgctgag
     4681 ccagtgggcc accgaggcct acgaaggcgc gcgtgagacg gtgcgccgct tcctcaatgc
     4741 ccgctcgacc cgcgaaatcg tgttcgtgcg tggcaccacc gaagcgatca acctggtggc
     4801 caacagctgg ggccgcgcca acctcaagag cggtgacgag attctgatca cctatctcga
     4861 gcatcactcg aatatcgtgc cgtggcagct ggtgtgcgcg gcgaccggag ccagactgcg
     4921 cgccgcgccc gtgcgaccca atggtgaagt cgatctggac gcgttccggg agatgctgaa
     4981 cccgcgcacc cgtctggtgg cagtggcgca cgtgtccaat gcgctcggca ccatccttcc
     5041 ggtgcatgac atcgtgcgcg tgagtcacgc ccgcggcgtg ccggtactgc tggacggagc
     5101 ccaggcggtt gcccaccacc gggtggacgt acaggacatc gattgcgatt tctacgcctt
     5161 ttccagccac aagatctacg ggccgaccgg catcggtgcg ctctacggca aggagtcgct
     5221 gctgtcagcg atgcccccgt ggcaaggcgg cggcgatatg attctgtcag tgactattga
     5281 atcgagcacc tggaacgatc tgccatggaa atttgaagcc ggcaccccca atatctcggg
     5341 tgcggtcggc ctgggtgcgg cgctccaata cgtggaatcc ttgggccgcg acgctatcgt
     5401 aacgcacgag caggagctgc tggcgcgcac gcaggaggca ctctcgcatc tgccgcgcgt
     5461 cacactgatc ggcaccgcac cctccaaagt ggcagtggtc tcctttacga tggagggggt
     5521 gcatccgcac gacatcggta cgattctcga cagcgaagga atcgccattc gcaccgggca
     5581 tcactgtgcg atgccgatca tcgagcaata cggattggcg gccaccgcgc gggcgtcgtt
     5641 cggttgctac aacaacgcct cggacgtcga acgtctggtc gcgggacttg ccaaagtgcg
     5701 cgacatgttt ggctgaagcg ggcgggtcag gagcacatgg atctcaaaga cctctatcgg
     5761 gaagtcattc tggaccacaa ccgccgcccg cgtaatttcg ggcgcgtgac gagcgccaat
     5821 gcggaagcga aaggtcacaa tccgctgtgc ggggacaagc tcgatctgac ggcgcgcgtc
     5881 cagggcgagc gcctggagga catccgcttc gaaggtcagg gctgcgcaat ctcgaccgca
     5941 tccgcatcgc tgatgaccga ggccgtgaag ggcaagaccc gccaggaaat cgcccaactg
     6001 ttcgaggagg tccatgagct gctgaccgat ccctcgcacg tgccagcgcg cgatctgggg
     6061 aaactgatgg cgctggccgg cgtggccgag tttcctgcgc gcgtcaaatg cgcgagcctg
     6121 tgctggcaca cgctcaacgc ggcattgggc agcgcctcgg ctccggtcag cacggagtaa
     6181 gccatgcgcg ttgccgagaa tgaaccggtc gtcatccacc gcgaggtcga tgcggtgatg
     6241 gtgccatctg gtcagccgat caagctcaag atgggactgg ccggctacat tactcaagca
     6301 ctgggcggca gcttcacgct gtacgtcgag ggcaatctgt atcgaattgc cggtgatcag
     6361 gctgatgcga tcggcaagga agtggtgctg ccacctacgc tgccgccgaa tgcgaccgaa
     6421 gaggacgtgc gcgagctggc ctgggagcag atgcgcggct gctacgaccc ggagataccg
     6481 atcaacatcg tcgatctggg cctggtctat gaatgcaagg tcacgcccaa tgaggaccac
     6541 acccgcaatc tcagcgtctc gatgacgctc actgcgcccg gctgtggcat gggcgacgtg
     6601 ctggtacagg acgtcaaaga gaagctggcg gcgattccca cggtacaggc ggtggacgtg
     6661 caactcacgt tcgatccgcc atggagccaa tccatgatgt cggaggctgc cagacttcag
     6721 accggaatga cgtggtagca ggcgtcgagc gtgaagcagc tgctactgtt gcgacacgcc
     6781 gaagcgcaac ccgcgtcccc tcacatcaaa gacttcgatc ggatactcac cgagcgcggc
     6841 cggcaggaag ccgcccaagc cgctgatgcc ctcgccgcag cgcgtttgac gatcgatgag
     6901 atcctggtca gcccctcgcg gcgcacgcgc gagacggccg atagtgtgat gcaccggctc
     6961 gggctcagga tgccggcccg ggcagtggcg gaactgtatc tggcagcacc cggcgtgatg
     7021 ctcgagacgc tcgaaggctg ccaggtcacc tcgagcacgg tgctattgat cgctcacaac
     7081 ccgggaatca gcgagctggc ccgtcagctc ggcggcggat cggggggcct ctcgctgcgt
     7141 accgccggcc tgtgtcatct gagcttggcg caggatcgat gggatgacat cggcagcgag
     7201 cgcgccagcg cctggcagtt actgcgctag cgctcacttg tgatgaagac gcgcgttgta
     7261 ctgtttgatg tgggcggtgt actggtcgag ctgggcggag tggatgcgtt gctcggctgg
     7321 cttggatcgg gtatgagcgc cgaacaactc tggacggtct ggttgcgttc cccggcggtg
     7381 cgcgcttttg agaccggccg gatagagccg ctagccttcg cgcacgctct tctctcagag
     7441 ctgggcctga cgatttcggc cgaggtgttt ctggaatcct tcgccacctg gccgacgcgc
     7501 ccgtatcccg gagcgctcga actggtggcc agcattcccc cgggctatca gcgcgcgcta
     7561 ctgtccaaca gcaacactct gcactggccc cgggtgatcg acgacatggc atttggctcg
     7621 agcttcgatc actgcttctc gtcccacctg acgggcaaga tcaaaccgga ccgcgaagca
     7681 ttcgagcacg tcgtgcatag gctcggctgc cagccctcgg agatcctgtt cttggacgac
     7741 aatcagttga atgtcgacgc cgcgcgccgg gtcggtatgc aggcgcagcg ggcacgcggt
     7801 gtcgaggagt cgcgactggc gctgcgacag gccggggtgc tttaggcggc gtcgccccaa
     7861 agcacggccc caccctccag gcccgcctcg ttggaaacca gactcacatt gtccggcagt
     7921 ttgaacgcga cacgcgtggc attgccgccg ccaatgtaga gatgatcgta atgcagcagt
     7981 gattcgagaa tctcgatcat gtgccgcacg cgcttgttcc agcgtttctt gccgtgtttt
     8041 ttgagggcgg cgttgccgac gtagtcgtta taggtcatgt cctcgtgaat cggatgatgc
     8101 gccagctcca ggtgaggcat gatctgacca tcacggaaca gcgccgtgcc ggcgccggtc
     8161 cccagcgtca gcaccaattc cagccccttg tgctcgatga cggccagtcc ctgcatctcc
     8221 gcgtcgttga tcagccgagc gggcgcgttg cccaaccgct gtgagagctc tttcgccaac
     8281 tcgaagccgg cccacttttc ggtgccccaa tgcggggcag taacgactcg gccgtcgcgc
     8341 accacacccg ggaagccgat cgcgatgtgg tcgtgtcgcg gaaacggttt cgccagtcgc
     8401 acgagcgcag ccaccatctg cttgggacga cagccctcgg gcgtgggcgt tttgagccgt
     8461 tctgtcagca tcctgccctt ggcatcgatg agggcggcct tcagattggt gccgccgata
     8521 tcaaacgcaa ggatgcgcga gagcccggcc gccatcagcg acgacctgta cccgccccgg
     8581 cgcgctgcag ttgtgcgcct ttggtcgcca gcacgctcat caggtcattc caggatttct
     8641 cgaacgcttt ggcgccgtcg cgctgcagat ccgcggccag ggccgcatcg ctcaccccag
     8701 ccttgttgaa cgcagcgatg acgctctcgg catcaccgcc gtccttcggc atgacgccac
     8761 tgaccttgcc gtgatcggcg aacgcgagca gtgtattttc gggcatggta ttgacggtat
     8821 tcggtgccgc cagtgcctcg atatacagcg tgtcggacgc cgccggatcc ttggtgccgg
     8881 tgctggcaag cagcaggcgc tgcggacggc cgccggcctt gatcaaggcc tgaccgcggg
     8941 gcgaggccca cagatccaga caggtcttgt aggtacgtct ggcgatcgcc acgcctaagc
     9001 gattgtgcat ttccttcggc accttgccgt tcgccgaacc atcccaacgg ctgatgaaca
     9061 cggaggcaac cgactcaatc ttcgggtcct tgcccgcctt gaggcgctgt tcgatacccc
     9121 tcatgtaggc ctcggcggcg gcctggtact ggtcggcgga gaacagcagc gtcacattca
     9181 ccggcacacc ggaggcaatc gactgctcga tcgccggcag gccttcacgc gtgcccggaa
     9241 tcttgatgta aaggttggga cgctttgcac gcccgtgcag ttcaatcgcg gccttgatcg
     9301 tgttcgcggt gtcataggcc agcagtggcg agacctccag cgagacgaac ccatctaccc
     9361 cgttggtcgc gcgatgcgcc ggcaggaaca gatcggcagc ctgcgtcaga tcctcgattg
     9421 ccagatcgaa gaacaacgct tcaccggacg aaccggcagc gcttttctgt gcgatcgcgg
     9481 catcgtagaa attgctgtct ttgatggcct tctcgaagat cgtcggattg gacgtcaatc
     9541 cggtcaccga tagctcgcgg atgtacttgg ccagcgtccc gctggtgagc attccgcgcg
     9601 tgatgttgtc gatccaaagg ctctgaccga gatcgtgaag ctgctgcgtg gctttcatgg
     9661 cgactccgac tccttaaggc aacttggcta atgcaggtgc actgccccac caaagaggcc
     9721 gtacagtcca atgagtatca gatagattgc gacgatgaaa ttgagcaatc gcggcagaat
     9781 caggatcaga atgccagcga tcagcgccgc gagagggccc aggtacagat gcaagctcat
     9841 gtttacttcc cctcaccagc gcctgatgcc agatgctggt aaaaattgca accaaatagt
     9901 caacagcgtt ttataaatat acgttgtccc gttcgttgca actgtcagcg cgcgtctgaa
     9961 ctatcgatgc tcagttacgc ttgctgtaca gtagcgtatt caatcgattg gatggagttt
    10021 atagatgcca aaagtcaaaa aagcacgttg gaagagaatt ctctggaccg ccgcggacgt
    10081 ccggctgctg aaaagtcagg ctcgccgtca acccgtgtcc aagatcgcga aacagcttcg
    10141 ccgatccgag gcagcagttc gttacaaagc caccacactc aagatctcat tggccctgcg
    10201 acaccggtga accgacatcg tgtgcgatat caatagattg cacacctaga aggttaccgc
    10261 ttcgagaacg ggggtcgttg gtctaaagct gctcgtccgc caatccaaga atggagtccg
    10321 cgccgtggac aaccgcgtgc gcgaggccgg aagctcgggg caacacggtg tcaccgtaga
    10381 accgcgcagt cactattctc gtttcgtaat attgcagatc gccctgccgc cgctgcagtc
    10441 ggcgctcagc ggccaacgcc cccctggcca gctgccagcc tccggcgacg acgccgaata
    10501 ggttcagcag tggttcggcg cccgcctgtg ccacatggat gtgctcgcgg ccacgttcca
    10561 ggacgaatcg aacggcttga tcgagcgact gaattgcgcg ctccagcgac cccgcgagcg
    10621 cgctcaggtg aacgtggtgc gaccgcgaca gctcgacctt gagctgcgac atcgacgcga
    10681 tcagatcgaa ggcggcccgc ccgccgtcac gcaatatctt gcgacccacg agatcattgg
    10741 cctgaattcc ggttgtcccc tcatagatag gtgtgatgcg ggcatcgcgc agatactggg
    10801 ccgccccggt ctcctcgatg aaacccatac cgccgtgaat ctgtacaccc aacgaggtca
    10861 gagtcaccgc cgactcggtt gaccagccct tcaccaccgg aatcagcagc gacatgaagc
    10921 gctgtgcccg ctgtcgcgcc tgcgcatcgg ggtgagcgcg cgcgtgatcg gttgcttcgg
    10981 ccaccacgcc ggccagggct cgcatggcct cgatcgtgga tttcatcgtc aacagcatcc
    11041 ggcgcacatc cggatgctgc acgatcgtga ccccaccctg gcgacgctcc ctcgcataac
    11101 ccagcgcctg ttgataggcg cgctccgcca ggcccagtcc ctgcacaccg acgctgaagc
    11161 gggccatgtt catcatgatg aacatgtatt cgagcccgcg attctcctcg cccaccaccc
    11221 aacccacggc tccgcgggtt gggtcggagc ccgcatcgtc gctgccgaac tgcatgacgc
    11281 aggtcggact ggcgtggatt ccgagcttgt gctcgatcga caggcagcgc acatcgttgg
    11341 ccgcgccgag cgcgccgtcc tccctgagca atagtttcgg gaccaggaac agcgatatac
    11401 ccttgacgcc ggagggagcg ttcggcgtac gtgccaggat c
//
LOCUS       DATMRM010000422         2559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2728197, whole genome shotgun sequence.
ACCESSION   DATMRM010000422 DATMRM010000000
VERSION     DATMRM010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2728197"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VNZ06_12845"
     CDS             <1..225
                     /locus_tag="VNZ06_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21686.1"
                     /translation="DLVNGNVVSGLSLGTSYAAAPKLVTEVGGAIAYTTNGSGENSNG
                     TCTSIPGTTSCIPGWTNKDSTSHGAWQEVR"
     gene            226..516
                     /locus_tag="VNZ06_12850"
     CDS             226..516
                     /locus_tag="VNZ06_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21687.1"
                     /translation="MKATIARKAVLVAAILVCSLPALAAKLVLPQVSGHVTAITANVS
                     ITIDGKQYAIAAGSPAATAVRSVQVGDVVGVMLDGPVGKATTHVTSILKQSN"
     gene            519..944
                     /locus_tag="VNZ06_12855"
     CDS             519..944
                     /locus_tag="VNZ06_12855"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HWW21688.1"
                     /translation="MRNAQRGFTMIELVVAMTVIGILVAVAIPSYNSYTLKSHRTDAI
                     RSLTSLRQALERCYSQNFTYVNAVTTPCPAAPGTAVLSANNYYSITYPTLAATSYSLL
                     ATAIGIQTKDGACATFTIDSTGKQASTGTGTTQTCWGSN"
     gene            1034..1561
                     /gene="aroK"
                     /locus_tag="VNZ06_12860"
     CDS             1034..1561
                     /gene="aroK"
                     /locus_tag="VNZ06_12860"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HWW21689.1"
                     /translation="MLGKRNIFLIGPMGSGKTAVGRSLSRLLDFPFYDSDHEIERRSG
                     VDVPLVFEREGEPGFRRREREVIADLTRQEPIVLATGGGAILDPHNRGDLAARGWVVF
                     LDTSITQQAERAGRTHHRPLLQGVDPTVRLGELMQVREPLYREIADYAVSTDQRRVNW
                     VAERVLREYKAARDT"
     gene            complement(1623..1790)
                     /locus_tag="VNZ06_12865"
     CDS             complement(1623..1790)
                     /locus_tag="VNZ06_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21690.1"
                     /translation="MSKFIGAALLMIGLSGAAMAVQTVGAPEIDPGSAMTGLAMLAGT
                     LAIVRGRRVRK"
     gene            complement(1914..>2559)
                     /locus_tag="VNZ06_12870"
     CDS             complement(1914..>2559)
                     /locus_tag="VNZ06_12870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HWW21691.1"
                     /translation="AAAADAGNPTAAYLLPFLAILAAGIVSRAGLPGFEKLYGLRLLA
                     ALLALAAYHRSFAALNWRFTWRGPLIGVAVCALWLVGAHFLSSHRDMPSELASLSPLA
                     RVLWISIRVAAAVITVPIAEELAFRAFLMRRLVSADFEGVPFVSVGVVPLLISSLAFG
                     ALHGALWPLAIAAGVAYGLVLIRTQALGEAVAAHATTNALIAVAVLVGGQWQLW"
BASE COUNT          513 a          792 c          814 g          440 t
ORIGIN      
        1 gatctggtga atggcaacgt cgtctcgggg ctgtcgctgg gtacttccta cgctgcggcg
       61 cctaaactgg tgacggaagt cggtggcgcg atcgcctata ccaccaacgg ttccggtgag
      121 aacagcaacg ggacctgcac ctcgattcct ggcaccacga gttgcatccc cggttggacc
      181 aacaaggaca gcacatcgca cggtgcatgg caggaggttc ggtaaatgaa agctacaatc
      241 gctcgcaagg ctgtgctggt cgcagccatt ctggtgtgct cattgccggc gctggcggca
      301 aagctggtgc tcccgcaggt gagcggccat gtcaccgcaa ttaccgcgaa cgtctccatc
      361 acgatcgatg gcaagcagta cgcgattgcg gccggcagcc cggcggcaac cgctgtgcgg
      421 tcagtgcagg tcggggacgt ggtcggtgtg atgctggacg ggcccgttgg caaggcaacc
      481 acgcatgtca ccagtatcct gaaacagagt aactagcgat gcgtaacgct cagcgcggtt
      541 tcacgatgat tgaactggtc gtcgccatga cggtcatagg gattttggtc gccgtggcga
      601 tcccctcgta caacagctac accttgaagt cccatcgcac cgatgccatc cgatccctga
      661 cgtcattgcg tcaggcgctg gaacggtgct attcgcagaa ctttacctat gtgaacgcgg
      721 tgaccactcc gtgcccggcc gctcccggca ccgcggtgct ctcggccaac aattactaca
      781 gcatcactta tccgactctg gctgcgacgt cgtattcctt gctggccacg gcgatcggga
      841 tccagaccaa agacggcgcg tgcgccacgt ttacgattga ttcgaccggc aagcaggcgt
      901 ccaccggtac cggcacgacc caaacctgct ggggcagtaa ctagcctgcg tttcgcgtcg
      961 cgcggcatct gcgactaaga tcacgcagca ccgattgaag gtgctgcgtc agggtctagc
     1021 ttcaggatgc cggatgctcg gcaagcgtaa tatctttctg atcggaccga tgggatcggg
     1081 caagacggcg gtcggcaggt cgctctcgcg gctgctcgat tttcccttct acgacagtga
     1141 ccatgagatc gagcggcgca gcggcgtcga cgttccgctg gtgttcgaac gcgagggaga
     1201 gcccggcttt cgccgtcgcg agcgagaagt cattgcggac ctgacccggc aggagccgat
     1261 tgttctcgcc accggtggag gcgccatcct ggatccgcac aaccgaggtg acctcgcggc
     1321 gcgaggttgg gtcgtgttcc tggacacctc gatcacccag caggccgagc gcgccggacg
     1381 cacgcaccat cggcccctgc ttcagggggt cgatccgacc gtgcgcctgg gcgaactgat
     1441 gcaggtgcgc gagccgctgt atcgcgagat tgccgactat gcggtatcga ccgatcagcg
     1501 gcgggtgaat tgggtggccg agcgggtact gcgcgaatac aaggctgctc gggatacttg
     1561 aagcgaggct ggcccggcag ctcgttgtgt ctgccggacc agctctcttt ggataagaag
     1621 acttatttgc ggacgcggcg tccgcgaaca atggcgagcg tgccagccag catggccagc
     1681 ccggtcatcg cagaacctgg atcaatttcc ggggccccca ccgtttgcac agccatcgct
     1741 gcaccgctca gcccgatcat cagaagtgcc gcaccgatga acttcgacat gttgtgtgtc
     1801 tccctgttgg tccctaagcg cccgaaaatc atcatagccc gaagggcggg ggaagaccag
     1861 atttatccgt gccaaggcat ttgcaatcaa tgagttaaca gaagacgcgg ttttcaccat
     1921 aactgccact ggccgcccac cagtacggca acggcgatca gagcgttggt cgtggcatgg
     1981 gccgcgacgg cctctcccag ggcttgggtg cgaatgagca cgaggccgta cgccacaccc
     2041 gcggcgatcg ccaacggcca cagcgcgccg tgcaaagcgc cgaaggccag cgaactgatc
     2101 agcagcggta cgacacccac gctcacgaat gggacgccct cgaagtccgc gctgaccaac
     2161 cggcgcatca ggaaggcgcg aaatgccagc tcttcggcga tcggtaccgt gatcacggcg
     2221 gcagcgaccc ggatgctgat ccagagcacg cgggccaatg gcgacagcga tgccagctcg
     2281 ctcggcatgt cacggtggga agacagaaag tgcgccccca cgagccagag cgcacacacc
     2341 gcgaccccga tcaggggtcc gcgccaggtg aaacgccaat tcagtgccgc gaacgagcga
     2401 tggtaggcgg ccaaggccag cagtgcggcc agcagacgca ggccatagag cttttcaaag
     2461 ccgggaagcc ctgctcggga gacaatgccg gccgcgagga tcgcgagaaa cggcaacaga
     2521 taggcggcgg tgggattgcc ggcgtcggcg gccgccgcg
//
LOCUS       DATMRM010000423         7283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3323442, whole genome shotgun sequence.
ACCESSION   DATMRM010000423 DATMRM010000000
VERSION     DATMRM010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3323442"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VNZ06_12875"
     CDS             <1..855
                     /locus_tag="VNZ06_12875"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWW21692.1"
                     /translation="AAAQLGAAAALVRSVGGADYRLPHTGMTTWDEKQQPIPAAALSA
                     EDADLVARLAAQGPVSMKLLLTPKTLPDADSANVIADWPGSERPNEYVVVSGHLDSWD
                     LATGATDDGVGVMAAAGVIQVLHQLNLHPRRTIRFIAWANEENGGRGREGYFNSVKDS
                     VASHVGAIESDTGAGRSLGLLASVTPQASMGLQPVLSALAPIGATALERRDDELGSDI
                     DPLQQVGVPGFAPLVDSRHYFDYHHTAADTLDKVDPQNLNTQVATMAVLAYFLAQLPE
                     SLPGVKTP"
     gene            944..1867
                     /locus_tag="VNZ06_12880"
     CDS             944..1867
                     /locus_tag="VNZ06_12880"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWW21693.1"
                     /translation="MTALLSACTTVLLTAANVPAMFAPVRRTAHLAFGPGARDRLDVY
                     QPSTASARPQPVIIFWHGGGWVDGQKSYYRFVGATLAQLGYVTILPNYRVYPEVRFPA
                     FLDDGARAVAWVQQHAADYGGDPHRIVLMGHSAGAHLAAMLAVNHSYLQHAGASTHDI
                     IGLIGLSGPYDLKPNTPVLNTIFSAPYSPRDWKVLPYVNADSPPALLIHGGADTLVWP
                     SNTLGMAAALRAHGVPVETRIYPGRSHADTVAALSVPARARGPVLKDVAEFMKQLSAT
                     GAATARPGRQSGLEAAAHPASRQPASLRSRS"
     gene            complement(1851..2468)
                     /locus_tag="VNZ06_12885"
     CDS             complement(1851..2468)
                     /locus_tag="VNZ06_12885"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWW21694.1"
                     /translation="MVISSSTAQLVRRTLVSLGVATALSVCMAAGAMAQEQTPTPHSD
                     SVGAAVSDTAITAKVKSSFVGDERLKGSHIKVVTTNGVVTLTGTAPTSDSKSAAEELA
                     QGVKGVRSVDDQVGTPASGGTVHKAVASTERVGSDSWITTKVKSEIMADSVTKGFDVH
                     VKTLHGVVMLRGTLPNKDAIDHVKDIAQKIDHVKSVDTTELRTAT"
     gene            complement(2538..2702)
                     /locus_tag="VNZ06_12890"
     CDS             complement(2538..2702)
                     /locus_tag="VNZ06_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002053759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HWW21695.1"
                     /translation="MLYYAIVFLVIALIAGALGFFGVAGAAVGIAKILFFVFLVLFVV
                     SLLIGRGRTL"
     gene            2965..3150
                     /locus_tag="VNZ06_12895"
     CDS             2965..3150
                     /locus_tag="VNZ06_12895"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWW21696.1"
                     /translation="MNKDQLKGRVEEAKGAIKETAGRVTGNADLKDRGTLEKAAGKVQ
                     KTYGDAKEEVKKEWKKP"
     gene            complement(3191..3970)
                     /locus_tag="VNZ06_12900"
     CDS             complement(3191..3970)
                     /locus_tag="VNZ06_12900"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HWW21697.1"
                     /translation="MSHALDRPLHGQPVPANRLHPRLYAAEAAGTALLVAVGLSIVIA
                     IFARHGPIAAVLPSPGARRALAGAGFGTIGALIAISPLGRLSGAHINPAVSLAFWLEG
                     KLAWRDLLGYVLAQMLGAAAGAVGLLLWGDIARELDFGATLVGSGVPLWLAFLGEAFA
                     TFALVLAIFISASHERTRAFTPWTIPPLFAWLVWWEAPLSGASANPARSFGPALISGL
                     WGDFWIYVFAPLMGAALGIGLLRLELLGQHRVEVARVARHD"
     gene            complement(3974..4756)
                     /locus_tag="VNZ06_12905"
     CDS             complement(3974..4756)
                     /locus_tag="VNZ06_12905"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWW21698.1"
                     /translation="MDLKLNGKRVLVTGGNSGLGAAMVRAFAAERARVAINYLVHPEV
                     TEQMASELKPSAGDVLGLQGDISKASDVTAMFQKIDAAWGGIDVLINNAGIDGPRALS
                     WEADPDAWNRVLQINLVGAFLCAREALKRMVPKKAGVIIHTSSVHEQIAWSGYSAYCA
                     AKAGLSMMSKTMAQEAGEFGVRVLCIAPGAIRTAINADVWKDPAGAQDLLSKIALQRV
                     GEAQDIAAMAVVLASDAAGYVTGTSVFVDGGMTDYPAFAHGG"
     gene            complement(4847..5554)
                     /locus_tag="VNZ06_12910"
     CDS             complement(4847..5554)
                     /locus_tag="VNZ06_12910"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HWW21699.1"
                     /translation="MCGRYITKEQRAIERELAYIDVVRWPDMEARYNVSPTQSVPVVR
                     WAEGQFEGTMMRWGLIPYFARGVAPKYSTINARLESIETAPAYRGPWKRAQRCVLPAS
                     GFYEWHLSESGARQPFYIHLADQEVFGFAGLWDRSIGPQGPVESCTLITMPANALLSQ
                     IHNVEHRMPAILQPADHEAWLRGDAAQARALLHEYPADTMVAWPVSTRVNSPRNDGAA
                     LIEPLVVQPPPEQSTLL"
     gene            5639..5956
                     /locus_tag="VNZ06_12915"
     CDS             5639..5956
                     /locus_tag="VNZ06_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21700.1"
                     /translation="MIRRRDPYNPLGKFSKPRWLIVWDMQFNVLDSTELAPYTDLAEV
                     MRGAAQRRRDEGWCVESDARFGSFFCHRDGMRVLVAISAADPAAATGGGPSWHESCPT
                     CGE"
     gene            complement(5966..6439)
                     /locus_tag="VNZ06_12920"
     CDS             complement(5966..6439)
                     /locus_tag="VNZ06_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21701.1"
                     /translation="MNPKYSIEEFERRWLADPSRLGEYQSCPHRIIQDRYLTGTGLRL
                     RKIEPVEGETLYKLGKKYGKSGSYVEPITNIYLSPQEYAALAGLDGRSVRKIRYAFAG
                     GSLDLYSNPNDGLIIFELEFPSVDAASRYLPPAFVREEITDNLRYSGAALAFPLV"
     gene            6479..>7283
                     /locus_tag="VNZ06_12925"
     CDS             6479..>7283
                     /locus_tag="VNZ06_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21702.1"
                     /translation="MSPSTFLRYLLLPATSFTVLVFIAALSLGFTLALHAGFLGLPLS
                     LLLSSWVFNYGFVVLEAIANGRREPPVLAIEMLNPLHEWRPMALVGMVFVTAMLLSVI
                     AVFGDARLAVALGIVFFVALPASTGALAVGSSTWQALNPAVVWQIVRALRFSYAAIVA
                     VVLLYGLLDWQLLRADIASWTVLDSGVFDPRGWMILAWAVFGWLSVFALIGGALYEHR
                     LELGHEPMDSPERRDERRQAETDRERARILDRVHAQARSGNLAGAWDTIQ"
BASE COUNT         1308 a         2334 c         2328 g         1313 t
ORIGIN      
        1 gccgcggcgc agctgggtgc ggcggcggct ctggtgcgct cggtcggtgg cgccgactac
       61 cgtctgccgc acaccggcat gaccacctgg gatgagaagc agcaacccat tccggctgcc
      121 gcgctgagcg cggaagatgc cgatctggtt gcgcggctgg ccgcacaggg acccgtgagc
      181 atgaagctgc tgctgacacc gaagacgctg cccgatgccg acagcgccaa cgtgatcgcc
      241 gactggcccg gcagtgagcg gccgaatgaa tacgtcgtcg tctccggtca ccttgattcg
      301 tgggatctgg ccaccggggc gactgacgat ggcgtcgggg tgatggccgc ggccggcgtc
      361 atccaggtac tgcatcagtt gaacctgcat cccaggcgca cgatccgctt catcgcctgg
      421 gccaatgaag aaaatggcgg tcgtggtcgc gagggctact tcaacagcgt gaaagacagt
      481 gttgcgagcc acgtcggggc gatcgagagc gataccggcg cgggccgctc gctgggcctg
      541 ctggcgagcg tcacgccgca ggcatcgatg gggctgcaac cggtattgag cgcgctggcc
      601 ccgattggcg ccacggccct tgagagacgc gacgatgagc tgggttcgga tatcgatccg
      661 ctgcagcagg tgggcgtccc cggcttcgcg ccgctggtgg attcgcgtca ctattttgac
      721 tatcaccaca ccgccgccga cacgctcgac aaggtcgatc cgcagaacct caacacccag
      781 gtcgcgacga tggcggtgct ggcctacttt ctggcacagc tgcccgaaag cctgcccggg
      841 gtcaagacac cttgagagca ccggcgtcgg tcgcatccac gttcagacat gcaacgtatt
      901 gttccggatg agcgggatcc ttaaaagagt tgcgctgctc gtgatgacag cactgctgtc
      961 ggcctgtacg acggtgctgc tgacagccgc gaacgtgccg gcgatgttcg cgcctgtgcg
     1021 ccgcaccgcc catcttgcct tcggcccggg cgcgcgcgac cggctcgatg tctatcagcc
     1081 ctcgacggcc tcggcgcgac cgcagccggt catcatcttc tggcatggcg gcgggtgggt
     1141 ggacgggcag aagagttact accgcttcgt gggtgccacg ctggcgcagc tcggctatgt
     1201 gactatcctg ccgaactatc gggtctatcc ggaagtgcgc tttccggctt ttctcgatga
     1261 cggcgcgcgc gcggtcgcct gggtacagca gcacgcggcc gactatggcg gcgatccgca
     1321 ccgcatcgtg ctgatgggac attcagccgg cgcccacctg gcagcgatgc tcgcggtcaa
     1381 tcacagctac ctgcagcatg ccggcgccag cacacacgac atcatcggcc tgatcggcct
     1441 gtccggccca tacgatctga agcccaacac gccggtgctc aataccatct tcagcgcacc
     1501 gtattcaccc agggactgga aagtgctgcc gtatgtgaac gccgattcgc cgccggccct
     1561 gctgatccat ggcggagcgg ataccctggt gtggccgagc aacaccctcg gcatggcggc
     1621 ggcgctgcgc gcacacggtg tacctgtgga aacgagaatc tatccgggcc ggtcacacgc
     1681 cgatacggtc gctgcgctgt cagttccggc gcgcgcacgc gggccagtgc tcaaggatgt
     1741 cgcggagttc atgaagcagc tctcggccac aggcgcagcc actgcgcgac ccgggcggca
     1801 gtcaggcttg gaggctgctg cacacccggc gtcgcgccaa cccgcctcac tcaggtcgcg
     1861 gtcctgagtt ccgtcgtgtc cacgctcttg acgtggtcga tcttctgcgc gatgtccttc
     1921 acatgatcga tcgcatcctt attgggcagc gtgccgcgca gcatgacgac gccgtgcagc
     1981 gtcttcacgt gcacatcgaa gcccttggtg acgctgtcgg ccatgatctc ggatttcacc
     2041 ttggtggtga tccagctatc ggatccgacg cgctcagtgc tggcgaccgc tttgtggacc
     2101 gtgccgccgc tcgccggcgt gccaacctga tcgtccacac tgcgaacgcc cttcacaccc
     2161 tgcgcgagct cctcggccgc cgactttgag tcggatgtcg gcgccgtgcc ggtcagtgtg
     2221 accacaccat tggtggtgac gaccttgata tgcgagccct ttaggcgctc atcgccaacg
     2281 aatgacgatt tcaccttggc ggtgatcgcc gtatcgctga ccgctgctcc gacgctgtcg
     2341 ctatgcggcg tcggcgtctg ttcctgcgcc atcgcccctg ctgccatgca aacactcagt
     2401 gccgtggcaa cgccgagcga caccagcgtg cgacgcacca gctgagcagt acttgatgaa
     2461 atgaccattg aaaatctcct gaaacctgga aggacgtgat tgttgacaaa actcccttaa
     2521 cactcgctta acggcagcta cagggttctg cctcggccga tgagcagcga aacaacgaac
     2581 agcacgagaa atacgaagaa cagaatcttc gcgatgccta ccgcggcgcc tgcgactccg
     2641 aaaaatccaa gcgcaccggc gatcaatgca atgacgagaa atacgatggc gtaatacagc
     2701 atgttgatct cctggtaacc gcaacggtcc tttgttatcg actcgacctt tccctgatcg
     2761 ccgcccatgc ggctgtggat agtccatgcg tcgttataca gccccgctgc gcgcgtgtag
     2821 ggcctcaatc agcgcatcgg actgtcggcg ccctctgatt cgaagaatgt caggagcctc
     2881 ctacgcagcg agtaccgtgc tgcacatggt gagcctgcat cgcgcgttca tactgactgc
     2941 acacactgtt agaaaggagt tgcgatgaac aaggatcaac tgaaggggcg cgtcgaggaa
     3001 gccaagggcg cgatcaagga gacagccgga cgcgtgacgg gcaatgcgga tctcaaggac
     3061 cgcggcacgc tggagaaagc agccggcaag gtgcagaaga cctacggcga tgcgaaggag
     3121 gaagtcaaga aggagtggaa gaagccttaa gcctcagccc gcgggacacg agcgagcgat
     3181 cactgttcgc tcaatcgtgt ctcgcgaccc gcgccacctc gacgcggtgc tggcccagaa
     3241 gctcgaggcg cagcaatccg atccccagcg ccgcacccat caacggcgcg aacacataga
     3301 tccagaaatc accccacagc ccgctgatca gcgccgggcc aaaactgcgc gccggattgg
     3361 cactggcgcc ggacaagggt gcttcccacc acaccagcca cgcaaacagc ggcggaatcg
     3421 tccagggtgt gaaggcgcgg gtacgctcgt gtgaggcgct gatgaagatc gccagcacca
     3481 gggcgaaggt ggcgaacgcc tcgccgagga acgcgagcca gagcggcacg ccgctgccga
     3541 ccaaagtcgc gccaaagtcg agttcacggg cgatgtcccc ccatagcagc agcccgacag
     3601 cccccgcggc ggcgcccagc atctgcgcca gcacgtaacc gagcagatcg cgccaggcga
     3661 gttttccctc gagccagaat gccagcgata ccgccggatt gatgtgcgcg ccgctgaggc
     3721 gcccgagtgg tgaaatcgcg atcagcgcgc cgatggtgcc gaagccggcg cccgccagtg
     3781 cgcggcgcgc gccgggcgag ggcaacacag cagcgatcgg accatggcga gcaaagatgg
     3841 caatgacgat cgacaggccc actgccacca gcagcgcggt gccggcggcc tccgcggcat
     3901 agagccgcgg gtgcagtcga ttggcaggca ccggctgccc gtgcagcggg cgatccagcg
     3961 cgtgcgacac gcttcaacca ccatgcgcga aggccggata gtcagtcatg ccgccgtcga
     4021 cgaacacact ggtcccggtg acgtagccgg ccgcgtcgga ggccagcacc accgccattg
     4081 cggcgatgtc ctgcgcttca ccgacgcgct gcagggcgat cttgctgagc agatcctgcg
     4141 caccggccgg atccttccag acatcggcgt tgatcgcggt gcggatggcg ccgggcgcaa
     4201 tgcacagcac gcgtacgcca aactcgcccg cttcctgcgc catggtcttg gacatcatcg
     4261 acaggccggc cttcgctgcg cagtaggcgc tgtagccgct ccaggcgatc tgctcgtgca
     4321 cgctgctggt gtggatgatg acgccagcct tcttggggac catgcgcttc agggcttcgc
     4381 gcgcacagag gaatgcgccg accagattga tctgcagaac acgattccag gcatccgggt
     4441 ccgcctccca cgaaagcgca cgcggaccgt cgatgccggc gttgttgatc agcacatcga
     4501 taccgcccca ggcggcgtcg atcttctgaa acatcgcggt cacatccgat gccttgctga
     4561 tgtcgccctg aagccccagc acatcgccgg cgctcggctt cagttcgctc gccatttgct
     4621 cggtgacttc cggatgcacc agatagttga tggccacgcg cgcgcgttca gcggcgaacg
     4681 cgcgcaccat cgcagcgccg agtccggagt tgccaccagt gaccaatact cttttgccgt
     4741 tgagcttcag atccatgtgg ggcctcgttg gctgtccatc gttggagacg cgcgtgcgag
     4801 catagcccag ctttgcgccg gacggtcacc tcaagacgag gctgacttac agcagcgtcg
     4861 actgctcggg aggcggctgc accaccagcg gctcgatcag ggccgcccca tcattgcggg
     4921 gtgagttcac gcgtgtactc accggccagg caaccatggt atcggccgga tactcgtgca
     4981 gcagcgcgcg cgcctgtgcc gcatcacccc tgagccaggc ctcatgatcg gcaggctgca
     5041 ggatcgcagg catgcgatgc tcgacgttgt gaatctggct cagcagcgca ttggccggca
     5101 tcgtgatcag ggtgcagctc tccaccgggc cctggggtcc gatcgagcga tcccagagac
     5161 ctgcgaaccc gaacacttcc tgatcggcca gatgaatata gaacggctgg cgcgcgcccg
     5221 actcgctcag atgccattca tagaagccac tggccggcag gacgcagcgt tgcgcacgct
     5281 tccacggacc gcgataggcc ggcgccgtct cgatcgattc gaggcgggcg ttgatcgttg
     5341 aatatttggg agccacgccg cgtgcgaaat acggaatgag cccccagcgc atcatcgtgc
     5401 cttcgaactg accctcggcc cagcgcacca cgggcaccga ctgggtcgga ctgacgttgt
     5461 aacgcgcctc catgtcgggc cagcgcacga cgtcgatata cgcgagctca cgttcgatcg
     5521 cgcgttgttc tttggtgatg taacggccgc acatgagatc tcacattaat tgtatacagc
     5581 ttgctgcggc gcggactact ggatggaccg ggcggcgtgg atgcctatgc tcacggacat
     5641 gatccgacgg cgtgacccct acaatcccct gggcaagttc tccaagccgc gctggctgat
     5701 cgtctgggac atgcagttca acgtgctcga ttcgaccgaa ctggcccctt acaccgatct
     5761 ggccgaagtc atgcgcggcg ccgcacagcg ccggcgggat gagggctggt gcgtcgagag
     5821 cgacgctcgg tttggttcgt tcttctgcca ccgcgatggc atgcgggtac tggtggcgat
     5881 ctcggcggct gatccggccg cggcaacggg cggcggtccg tcgtggcatg aatcctgtcc
     5941 gacctgcggt gaatgaagcg ccgctctaga cgagcggaaa tgccagcgcg gcgccgctgt
     6001 agcgcagatt gtcagtgatc tcctcgcgca cgaacgccgg cggcaggtag cggctggcgg
     6061 catcgaccga ggggaactcg agttcgaaga tgatcagccc gtcgttcggg ttggagtaga
     6121 ggtcaagcga cccgccggca aaggcgtaac ggatcttgcg taccgagcgg ccgtccagtc
     6181 ctgcgagcgc tgcgtattcc tgcggcgaga gatagatatt ggtgatcggc tcgacgtagc
     6241 tgccgctctt gccgtacttc ttgccgagtt tgtagagcgt ctcgccctca accggctcga
     6301 tcttacgcag tcgcaggccg gtgccggtca gatagcgatc ctggatgatc cggtgtgggc
     6361 agctctgata ctcgcccagc cgcgatggat cggcgagcca gcgccgctcg aattcctcga
     6421 tcgaatactt ggggttcatc taagatcgaa gtttaatccc gcggctaccc tgttagcgat
     6481 gtcgccgtcc acattcttgc gctatctgtt gctgccggcg accagtttta cggtgctggt
     6541 attcatcgcc gcgctctcgc tgggcttcac actggcactg cacgcgggct ttcttggctt
     6601 gccgctgtcg ctgctgctga gttcgtgggt attcaactac gggttcgtcg tgctcgaagc
     6661 gatcgccaat ggtaggcgcg aaccaccggt gctggcgatc gagatgctca atccgctcca
     6721 tgaatggcga ccgatggcgc tggtaggaat ggtcttcgtg accgccatgc tgctgagcgt
     6781 gatcgcggtg ttcggggatg cgcggctcgc cgttgcgctg gggattgtgt tctttgtcgc
     6841 actgccggcc tccactggcg cgctggcggt cggctcgagc acctggcaag cgctcaatcc
     6901 ggcggtggtg tggcagatcg tgcgtgcact gcgcttcagc tatgcggcga tcgtcgccgt
     6961 ggtgctgctc tacggtttgc tggactggca gctgctgcgc gccgacatcg cgagctggac
     7021 ggtgctcgac tcgggagtgt ttgacccccg gggctggatg atcctcgcct gggcagtctt
     7081 tgggtggttg tccgtgttcg cgctgatcgg cggcgcgctg tatgaacatc gccttgaact
     7141 cggccacgag ccgatggatt caccggaacg tcgtgacgag cgccggcagg cggagaccga
     7201 ccgcgagcgc gcccgcattc tggatcgggt gcacgcgcag gcgcgcagcg gcaatctcgc
     7261 cggcgcctgg gacacgatcc agg
//
LOCUS       DATMRM010000424         6148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2102822, whole genome shotgun sequence.
ACCESSION   DATMRM010000424 DATMRM010000000
VERSION     DATMRM010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2102822"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            304..1602
                     /locus_tag="VNZ06_12930"
     CDS             304..1602
                     /locus_tag="VNZ06_12930"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWW21703.1"
                     /translation="MSKPHDANGVNSVVDGRYVLEQPLGGGGMGVVYRARDKLMEKHR
                     DRDPYVALKLISESLKGDEQARSLLQRECSRAQRLSHPSIVRVFYFGCDPTTDTDYLT
                     MELLRGSSLDRLIKANPQGLAWDRAAPLIEALLSGLHYAHAEGIIHSDIKPSNLFVTE
                     SGHLKILDFGIAAPVRNADTTRGETLFNPRRMGAVAPRHSSLEMFLGKDADASDDVYS
                     AACVIYELVTGKHPYKGLETPRAAELNLVPDTVRSLSRAQNSALCKGLAFRRSERAAT
                     IAELRLGLFPPARPAATRRTAPYAIAAGTVVALVLVAGYRMAAPDRVSASRPAALPHA
                     PALTSAPALRTSVTTPDPAPTPASEAAAAEPAASTTLPRIVAPAGRLPSHAINPSSTK
                     AQTPSTKKSLSGRCQSILERQGLGEFNEDDKNYLDANCRR"
     gene            1559..2923
                     /locus_tag="VNZ06_12935"
     CDS             1559..2923
                     /locus_tag="VNZ06_12935"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWW21704.1"
                     /translation="MKTTRTISMPTAAVSASVRLALSLIAFGAIAGCVPMQGEGPRPS
                     ELDRMVAATKQAPPAPTPAAAPVASAPGTEAAPAPPAPPATPPPLPPILPFDEAITTA
                     ARNVFKAALAQDPNAKFVVIDPLIDGVTGYQSKATRTIQDVIIKVARQDFPQYLVKSI
                     TPEILEQKPRILVGTFTPVNAQNKPAGEREAFRFCLVMGDLKTGKVIAKSVVRTPIAQ
                     ADSTPTSTFGDSPVWSEDASVQAYIATCQGSKVGDPIKAEYVGGLFAAALINEASQAY
                     DEGRYSEALDLYRTAHKADAGDQLRVYNGIYLSLMKLGKMDQAQVAFKDLVDYGLRNK
                     RLAVKFTFRPGSVRFANDPKTTNTYDMWLHEIASEASDKRVCLHITGHTSPTGPAALN
                     QELSLERAEYVQTKLEGDAPYLQKRTTAEGRGSRDNIIGTGRDDASDAIDRRVELNPI
                     EPCT"
     gene            complement(2979..3692)
                     /locus_tag="VNZ06_12940"
     CDS             complement(2979..3692)
                     /locus_tag="VNZ06_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21705.1"
                     /translation="MSEPQEHFWFQSLEQRAELAGLALRGAFHPASGEFEQLSSLGPL
                     GTVILLGFTADLQWPIFAQSSEAKDGLAHPLDRWSHRVIGALAREVGARDFYPDDAPR
                     LPFQQLAMRCEAVHPSPIGLLIHPRWGLWHAYRGGLTFADRLPLPPRTSAPSPCASCE
                     AKPCLNACPVGAFSFNGYHLAACVAHVSSLAGAQCRQGGCLARHACPVGPAPGYAPQQ
                     RQFHMNAFLAAFKAGDRAG"
     gene            complement(3707..4237)
                     /locus_tag="VNZ06_12945"
     CDS             complement(3707..4237)
                     /locus_tag="VNZ06_12945"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWW21706.1"
                     /translation="MFEFQPELIGQIIQLRPLRPADFDSLCQVASDPLIWDQHPDKSR
                     STMAGFRTFFANALESGGTLVAVDRRQRLTIGCSRFHAYREPQNDVIVGYTFLARSHW
                     GGTYNAEMKRLMLDHAFGFVENVRFLIAEQNLRSRRAIEKLGAVCIDSEVHQELRIVH
                     LVYQLGRAQYLGPQSK"
     gene            complement(4254..5963)
                     /locus_tag="VNZ06_12950"
     CDS             complement(4254..5963)
                     /locus_tag="VNZ06_12950"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HWW21707.1"
                     /translation="MGTRCLSLAQLIGGMILSISSFSVAADPADLRKIAHDYYQWRDA
                     AYPVATSGLGDHRFDSRLTDYRMSEITARRQHISELIAQADALKTDGWSRDDRVDLVL
                     FQSQLAAADFFDRRFDPESSDPQLYVNECSNAIFSLLQKDYADHRTRALAAIARLEQM
                     PALLQTARTNLTQPVKLYAQLAIQAARGGDELYTVSLMTLADRPSSAERKRLVAARDK
                     AIRALHDFADWLSTSLPKMPDWRPMGEESYNYLLKHILLLPFDAHDVAHLGEVELARY
                     RALEAMLKDPNLASPDPARAAHVPKDQAEFLAAYQARLKEIVDFLNANHLVTIPTYIG
                     PFTIRQLPDAFKPTSPGGFMNPPGIYDQDPGGFYFIPAYNPKSGNFYIRAAIEDPRPI
                     LGHEGIPGHFLQLSVANHLTDEIRRQHSDSVFVEGWALYGEEMMLREGLYPTNSAGAA
                     QVLRLARYRAARIGVDVNLQTGRWTFEQAVKYFMEAGGLDREAAEGEAAGAASNPSQK
                     ICYITGKWQIMRLLGRYRDRQGANFRLGAFHDQLISYGSLPLSVIEWLMFDDDSSVRA
                     ALQ"
BASE COUNT         1284 a         1898 c         1853 g         1113 t
ORIGIN      
        1 tgcgcccgca gcggaactgg ctgtgtcgtg gggctgacag aagcgcggca acctcatcgc
       61 cagcatcgcc ccatgggtca tgcagcgaca ttcagctcgc agttcgctag ccagttgagc
      121 accttgccga tggcaggcgc tagcagcggc gcgccgccgc gctactcaaa aaaagccgaa
      181 cgtcgtacga taaacaccgt acctcgagcg ctcaaggaag gcgtgtccag gccacccgca
      241 aaggccggaa cgactgcagt ggatatcagg gacccggtcg cccaggcggc gacggagctg
      301 tcagtgtcta aacctcatga cgccaatggc gtcaattccg tggtggatgg tcgctacgtt
      361 ctcgaacagc ctctcggcgg cggcggcatg ggtgtggtct atcgcgcacg cgacaagctc
      421 atggaaaagc atcgcgaccg cgacccttac gttgcgctca agctcatcag cgaatcgttg
      481 aaaggggacg agcaggcgcg ctcgctgctg cagcgtgaat gcagccgtgc acaacgattg
      541 tcccacccga gtattgtgcg cgtgttctat tttggttgcg acccgacaac cgacaccgac
      601 tacctgacga tggaactgct gcgtggcagt tcgttggacc gtcttatcaa agccaaccct
      661 caggggctgg cctgggatcg ggcagcaccc ctgatcgaag cgctgctcag cggtctgcac
      721 tatgcgcacg ccgaaggcat catccattca gatatcaagc ccagcaacct gttcgtgacc
      781 gagagcggtc atctcaagat tctggacttt ggcattgccg cgccggtgcg caatgccgac
      841 accacccgcg gcgagacgct gttcaacccc cgacgcatgg gcgccgtggc accgcgccat
      901 tcatcgctcg agatgttcct gggcaaagat gcggacgcca gcgatgacgt ctatagcgcc
      961 gcgtgcgtca tctacgaatt ggttacgggg aaacacccgt acaaagggct ggaaaccccg
     1021 cgcgccgccg agctcaatct cgtgcctgac accgttcggt cactgagtcg ggcacagaat
     1081 agtgccctat gcaaaggact tgcgtttcgc cgctccgaac gcgcggcaac gatcgcggaa
     1141 ctgcgcctgg gactgtttcc ccctgctcgc cctgcagcaa ccaggcgaac ggcgccctac
     1201 gctatcgcgg caggcaccgt cgtcgcactg gtgctggtgg cgggctaccg gatggcagcg
     1261 cccgatcgcg tatcggcatc acgacccgcg gcactgccgc acgcgcccgc tctgacgagc
     1321 gcgccggcct tgcgtacatc ggtcacaaca ccggacccag caccgactcc cgcgtcggag
     1381 gctgcggccg cagagcctgc ggcgtcgacg acgctgccga gaatcgtggc cccggcagga
     1441 cgtcttccga gccacgctat caatccatca tccaccaagg cgcagactcc gtcgacaaag
     1501 aagtcgctca gcggtcgctg tcagtccatc ctcgaacgac aaggtttagg agaattcaat
     1561 gaagacgaca agaactatct cgatgccaac tgccgccgtt agcgcttcag tacgactcgc
     1621 actgtcgctc atcgccttcg gggcgatcgc tggctgcgtg ccgatgcagg gtgagggacc
     1681 tcgtccttcc gaactcgata ggatggtggc cgcgactaag caggctcccc cggcgccgac
     1741 ccccgccgcc gcacccgtcg cgtctgctcc ggggaccgag gcggcccccg ctccgcccgc
     1801 cccgccggcg acaccgccgc cgctgccgcc gatcctgccg tttgacgaag cgatcaccac
     1861 cgctgcgcgc aatgtcttca aggcagcgct ggcgcaggat cctaacgcca agtttgtggt
     1921 gatcgacccg ctgatcgatg gggtcaccgg ctatcagtcc aaggccacgc gcaccattca
     1981 ggacgtgatc atcaaagtgg ccaggcagga ctttccgcaa tacctggtga agagcatcac
     2041 accggagatt ctggagcaga agccgcgcat cctggtggga acgtttaccc cggtgaatgc
     2101 gcagaacaag cccgctggcg agcgtgaagc ctttcgtttc tgtctggtga tgggtgacct
     2161 caagaccggc aaggtcatcg ccaaatccgt ggtgcgcact ccgattgccc aggctgactc
     2221 gactccaact tccaccttcg gcgacagccc agtgtggtcg gaggacgcca gcgtccaggc
     2281 ctacatcgcg acctgccagg gcagcaaggt gggcgatccg atcaaggccg aatacgtcgg
     2341 aggactgttt gcggcggcgc tgatcaatga agccagtcag gcgtatgacg aaggtcgcta
     2401 cagcgaggcg ctggatctgt atcgcaccgc tcacaaggcc gatgccggcg atcagctacg
     2461 cgtgtacaac ggcatctatc tgtcgctcat gaagctggga aagatggatc aggcgcaagt
     2521 cgctttcaag gatctggtgg actacggcct gcgcaacaag cgattggcgg tcaagttcac
     2581 gttccggccg ggtagtgtcc ggtttgcgaa tgatcccaag accaccaata cctacgacat
     2641 gtggctgcac gagattgctt cggaggcttc cgataagaga gtctgtcttc atatcaccgg
     2701 acataccagc ccgacgggcc ctgcagccct gaaccaggag ctatcgctcg aacgcgccga
     2761 gtatgtgcag accaagctgg aaggcgatgc gccttatctg cagaagcgca ctaccgccga
     2821 aggacgagga tcgcgcgaca acatcatcgg cactgggcgc gacgatgcgt ccgatgcgat
     2881 cgatcgcaga gtggagctca atccgatcga accgtgcacc tgagcttcca acgacgcgtt
     2941 gaatgacgct actgccgtgg atgacccgcg cccaccgatt agcccgcgcg atcacccgcc
     3001 ttgaacgccg cgagaaacgc gttcatgtgg aactggcgct gttgcggagc atagcccggc
     3061 gcaggtccga ctggacaggc atgccgagcg agacagccgc cttgtcgaca ctgcgcaccc
     3121 gcgaggctcg agacgtgcgc tacgcaggcc gcgagatggt agccgttgaa tgaaaacgct
     3181 cccaccgggc acgcattcag acaaggtttg gcctcgcaac tcgcgcacgg gctaggcgcc
     3241 gaggtacgcg gcggcaacgg caggcgatca gcaaaagtga gcccgccgcg atacgcgtgc
     3301 cacagacccc agcgcggatg aatcagtagc ccaatcggcg acgggtggac cgcttcgcac
     3361 cgcatcgcaa gctgttgaaa cggaagccgg ggcgcatcat cgggatagaa atcgcgagcg
     3421 ccgacctcac gggccaacgc gccaatgacg cgatgtgacc agcggtcgag cggatgcgcc
     3481 aggccatcct tggcctccga ggactgtgcg aagatcggcc attgcagatc cgcagtgaat
     3541 cctagaagaa tgaccgttcc aagggggccc agcgaagaga gctgttcgaa ctcgcccgat
     3601 gccggatgga aggcgccgcg gagcgcaaga cccgccagct cggcgcgttg ctccaggctc
     3661 tgaaaccaga aatgctcttg cggctcggac actgaactca gctgtctcac ttcgactgcg
     3721 ggccgagata ctgcgcgcgg ccgagctgat acaccaggtg aacgatcctc aattcctggt
     3781 gaacttcaga gtcaatgcaa accgcaccga gtttctcgat ggcgcgtcgc gagcgcagat
     3841 tctgctcagc gatcaggaat cgcacgttct ctacgaatcc aaacgcgtga tcgagcatca
     3901 ggcgtttcat ttctgcgttg taggttccac cccagtggct gcgagccagg aacgtatatc
     3961 caacgatgac gtcattttgc ggctcgcgat aggcatgaaa tcgtgagcaa ccgatcgtga
     4021 gccgttgtcg gcgatcgacc gcgacgaggg taccgcctga ctccaaggcg ttggcgaaga
     4081 atgtcctgaa gcccgccatc gtgctgcgtg acttatcggg atgctgatcc cagatcagcg
     4141 gatcggaagc gacctgacag agcgagtcga agtcggcggg ccgtaacggg cgcaactgga
     4201 tgatctgtcc gatcagctcc ggttgaaact caaacatggg ttgtctcgct gactcactgt
     4261 agtgccgcac gcaccgagct gtcatcatcg aacatcagcc attcgatgac ggaaagcggc
     4321 agagagccat agctgatcag ctgatcgtga aaggccccca aacggaagtt ggcgccctgc
     4381 cgatcacgat agcgacctag cagacgcatg atctgccact ttccagtgat gtagcagatc
     4441 ttctgtgaag gattcgaggc cgcaccggcg gcttcccctt cggctgcctc acgatccagt
     4501 ccgcccgctt ccatgaagta cttcacggcc tgttcgaagg tccagcgacc cgtctgcagg
     4561 ttcacatcca ctccgatccg tgctgcacga tagcgcgcca gtcgcagcac ctgggcagca
     4621 cccgcggaat ttgtcgggta cagaccttcg cgcagcatca tctcttcacc gtagagtgcc
     4681 cagccttcga cgaacacact gtcggagtgc tgacgacgga tttcatcggt cagatgattg
     4741 gcgaccgaga gttgcagaaa gtggccgggg atgccttcgt gacccaggat cgggcgcggg
     4801 tcttcaatcg ctgcgcggat gtagaaattt ccgctcttgg ggttgtaggc cggaatgaaa
     4861 tagaacccgc ccggatcctg atcatagatg ccgggaggat tcatgaaacc accgggcgag
     4921 gtaggcttga acgcgtcggg aagctgacga atggtgaacg gcccgatata cgtgggaatg
     4981 gtcaccagat ggttggcatt caagaagtca acgatctcct tcaagcgcgc ctggtaagcg
     5041 gcaaggaact ccgcctgatc cttcggaacg tgcgcggcgc gtgcgggatc gggtgacgca
     5101 agattgggat ccttgagcat cgcctcgagc gcgcgatagc gcgcgagctc aacctcaccc
     5161 agatgcgcca cgtcgtgtgc gtcgaacggc agcagcagaa tgtgcttcag cagatagttg
     5221 tagctctctt cacccatcgg ccgccagtcg ggcattttcg gcaggctggt ggaaagccag
     5281 tccgcaaagt catgcagcgc ccggatggct ttgtcgcgcg ccgcaaccag tcgcttgcgc
     5341 tcggctgacg acggccgatc ggccaatgtc atcaggctga cggtgtagag ttcatcgcct
     5401 ccgcgcgccg cctggatggc aagctgcgca tagagcttga ccggctgcgt gaggttggtg
     5461 cgtgcggttt gcagcagtgc aggcatctgc tcgaggcgcg caattgccgc cagggctctc
     5521 gtacgatgat cggcgtagtc cttctgcagc agactgaaga tcgcattgct acattcgttg
     5581 acatagagct gcggatccga ggattccgga tcaaatcggc ggtcgaagaa gtcggcggcc
     5641 gccaactgcg attgaaacag caccagatca acgcggtcgt cgcggctcca cccatcagtc
     5701 ttcaacgcgt cggcctgcgc gatcagttcg ctaatgtgct gacgccgcgc ggtaatctca
     5761 ctcatccggt aatcggtgag tcgcgaatca aatcggtgat cgcccagacc gctcgttgcc
     5821 accggatagg ccgcgtcacg ccattgatag tagtcgtgcg caatcttgcg aagatctgca
     5881 gggtcggctg caacactgaa cgaactgatg gaaaggatca ttccgccgat gagctgcgcc
     5941 aaggacagac agcgtgttcc catagttcgc tttccgctga gatggactga tgcgcaagtg
     6001 tgatcgcaat tgggtatatg tcaaagcacg cgtcgcccat gtactttgta agacaaagta
     6061 cttgacgtca tttgttttga cgcatagagt gaacgccatg gtcgcacctg tgaaatccgt
     6121 cctgtctgcc ggcaacccca tctccatg
//
LOCUS       DATMRM010000425         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3324135, whole genome shotgun sequence.
ACCESSION   DATMRM010000425 DATMRM010000000
VERSION     DATMRM010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3324135"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VNZ06_12955"
     CDS             <1..700
                     /locus_tag="VNZ06_12955"
                     /inference="COORDINATES: protein motif:HMM:NF021472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HWW21708.1"
                     /translation="PIEWLSRGWDDLKQIGRAGLAHGLLIAILGAVLLMLGSTHLYLT
                     AAAVTGYLLVGPVMTTGLCELARRRAAHEPLGFDESLQGLTRNPDGLMHFGGVLALIA
                     LSWFVLSAFLLQSVLNASVPSLAVALWGGSADVSAAQILGYVGCGAVLAGTVFVMSVV
                     AVPLIIDRHASASDAIRASLRATLLNLPAMLVWAVFIVGLTVLGFVTLLAGMVIVAPL
                     LGYATWHAYRDLIA"
     gene            782..1087
                     /locus_tag="VNZ06_12960"
     CDS             782..1087
                     /locus_tag="VNZ06_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21709.1"
                     /translation="MTASSITEGHNEFLTGLQIIEREIIRQVREAGGQIGPSNFQWHE
                     GKDLLPPPSAIALKISTPERTINATFSRGAVQDSRDRVDRGDVRARIRALVEELTKL"
     gene            1360..1692
                     /locus_tag="VNZ06_12965"
     CDS             1360..1692
                     /locus_tag="VNZ06_12965"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWW21710.1"
                     /translation="MAQRHDTDVLFKALADPSRRKLLDLLHAHDGRTLNELCEHLDMT
                     RQGVTQHLDLLEAANLVATVRRGREKLHFLNPVPLQEIYERWIAKFEKPRLKALSDLK
                     QRLENADG"
     gene            1685..>2145
                     /locus_tag="VNZ06_12970"
     CDS             1685..>2145
                     /locus_tag="VNZ06_12970"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWW21711.1"
                     /translation="MARSTFVYVTYIRTTPEKLWSALTDDVEFMKQYWFGTHCESQWK
                     AGSSWKMVSGDGHITDAGEIVEAEPPRRLVIRWQHQVKPELKAEGESLCTMVLEPSGT
                     AVKLSITHTIEHEPSKLIEAVSGGWPKILSNLKSLLEAGSAVLKDPYPTGN"
BASE COUNT          400 a          658 c          696 g          391 t
ORIGIN      
        1 acccatcgag tggctgagcc gcggctggga cgatctgaag cagatcggcc gcgccggtct
       61 cgctcacgga ctgctcatcg cgattctggg agcggttctc ctgatgctcg gcagcaccca
      121 cctgtacctc accgcagctg ccgtcacagg ctatctgctc gtcggtccgg tgatgaccac
      181 cggtctgtgc gaactcgcgc gacggcgcgc ggcgcacgag ccgttgggtt ttgatgagtc
      241 gctgcaggga ctgacgcgca accccgacgg cctaatgcac ttcggaggcg tgctggcgct
      301 catcgcgctc tcgtggtttg tcctgtccgc cttcctgctg cagtcggtgc tcaatgcttc
      361 ggtgccgagc ctcgcggtcg cgctgtgggg cggatccgcc gatgtgagcg cggcacagat
      421 cctgggctac gtgggatgcg gcgccgtgct cgcgggcact gtcttcgtga tgtcggtcgt
      481 ggccgtgccg ctgatcatcg accggcacgc cagtgcgtcg gatgcgatcc gcgcgagtct
      541 gcgtgcgacc ctcttgaacc tgcccgcgat gctcgtgtgg gcggtcttca ttgtgggact
      601 caccgtgttg ggtttcgtga ctctgctggc cggcatggtg atcgtcgcgc cgctgctcgg
      661 ctatgcgacc tggcatgctt accgggacct catcgcctag acgcgtgcga agcgcacctg
      721 cggcggggtg gccgtggacg ggtacccggc cccggagcag acgaactgag gaggtgcgcg
      781 catgacggca tcgtcaatca ccgagggaca caacgagttc cttaccggcc tgcagattat
      841 cgagcgcgag attatcaggc aggtgcgcga agccggtggg cagattggcc cgagcaactt
      901 tcagtggcac gaagggaagg atctgcttcc gccgccaagt gcgatcgcgc tgaagatcag
      961 cacgccagaa agaactatca acgcaacgtt cagccgcgga gcagtgcaag actcccggga
     1021 cagggttgat cggggcgacg ttcgggcacg gattcgcgcc ttggttgagg aactcacgaa
     1081 actctgagac gccggccaac gcgcaccccc gcccgtgctc ccaagggatg cgtacccgcc
     1141 gtgtcggccg acggctacgc ggcctgactg atagccctgt cccagtcttc gatcagctgc
     1201 ctaccgaata gacgatgacg tcgatccagc ggtagaccag cagcgccaac ccgcgaagcg
     1261 gcctcgacta gtgggcgcgt tgtcgatcgt tgaccagcgc ttgacacgca aggcgcgact
     1321 tgcctatcat ttaggcaagt aagtacttgc gtataacaca tggcacagcg ccacgacacc
     1381 gacgtgcttt tcaaggccct ggccgatccc agccggcgca agctgctgga cctgctgcat
     1441 gcgcacgacg gccgcacact caacgagctg tgcgaacacc tggacatgac gcggcagggc
     1501 gtgacgcagc acctggactt gctggaggcg gcgaacctgg tcgccaccgt gcggcgcggc
     1561 cgcgaaaagc tgcacttcct caatccggta ccgctgcagg aaatctacga gcgctggatt
     1621 gccaagttcg aaaagccgcg cctcaaggcg ctttcggatc tgaaacaacg actggagaat
     1681 gccgatggct agatcgactt ttgtctacgt gacctacata cgcaccacgc cggagaagct
     1741 gtggtcagcg ttgacagatg acgtggagtt tatgaagcag tactggttcg gcacgcactg
     1801 cgagagccag tggaaggcgg ggtcctcgtg gaagatggtg tccggggacg gacacatcac
     1861 cgacgccggt gagatcgtcg aggctgagcc accacggcgg ttggtgattc gctggcagca
     1921 tcaggtcaaa cccgagctca aggctgaagg tgagtcgctg tgcacgatgg tgctggagcc
     1981 cagtggaacg gcggtgaagc tttccatcac acacacaata gagcacgaac cctcgaagtt
     2041 aattgaggcc gtctctggcg gctggccgaa gatcctctcc aacctgaagt ccttgctgga
     2101 ggcgggctcc gcagttctga aggacccgta cccgacgggc aatca
//
LOCUS       DATMRM010000426         4744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1828614, whole genome shotgun sequence.
ACCESSION   DATMRM010000426 DATMRM010000000
VERSION     DATMRM010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828614"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..315
                     /locus_tag="VNZ06_12975"
     tRNA            230..315
                     /locus_tag="VNZ06_12975"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:264..266,aa:Tyr,seq:gta)
     gene            384..457
                     /locus_tag="VNZ06_12980"
     tRNA            384..457
                     /locus_tag="VNZ06_12980"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:416..418,aa:Gly,seq:tcc)
     gene            542..617
                     /locus_tag="VNZ06_12985"
     tRNA            542..617
                     /locus_tag="VNZ06_12985"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:575..577,aa:Thr,seq:ggt)
     gene            646..1836
                     /gene="tuf"
                     /locus_tag="VNZ06_12990"
     CDS             646..1836
                     /gene="tuf"
                     /locus_tag="VNZ06_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWW21712.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAALTKVMAETHGGTF
                     VAYDQIDKAPEEKARGITISTAHVEYQSKARHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPHIVVFMNKADMVDDKELLELVEMEVRELLTT
                     YKFPGDKTPIIIGSALKALEGDKSEIGVAAVVKLVEEMDKYIPVSKREVDKPFLMPVE
                     DVFSISGRGTVVTGRIERGIVKVNDEVEIIGLKATQKTTCTGVEMFRKLLDEGQAGDN
                     VGVLLRGTKREEVERGQVLAKPGSITPHTHFECEVYVLTKEEGGRHTPFFKGYRPQFY
                     FRTTDVTGMIELPGNTEMVMPGDNIQMTVELIAPIAMEQGLRFAIREGGRTVGSGVVS
                     KILK"
     gene            1869..2180
                     /gene="rpsJ"
                     /locus_tag="VNZ06_12995"
     CDS             1869..2180
                     /gene="rpsJ"
                     /locus_tag="VNZ06_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HWW21713.1"
                     /translation="MANQNIRIRLKAFDHRLIDNSAKEIVDTARRTGATVRGPVPLPT
                     KMERFTLLISPHADKDARDQLELRTHKRLMDILNPTDKTVDALMRLELPAGVDVQIRL
                     N"
     gene            2435..3070
                     /gene="rplC"
                     /locus_tag="VNZ06_13000"
                     /pseudo
     CDS             2435..3070
                     /gene="rplC"
                     /locus_tag="VNZ06_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
     gene            3163..3768
                     /gene="rplD"
                     /locus_tag="VNZ06_13005"
     CDS             3163..3768
                     /gene="rplD"
                     /locus_tag="VNZ06_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HWW21714.1"
                     /translation="MKLKVSNGGAEIAVSDENFGREYNPALVFQVVNAYRAAGRAGTK
                     AQKTRAQVRGGGRKPHAQKGTGSARAGSIRSPIWVGGGRTFAAQPRDFSQKVNRKMYR
                     GAIRSMLSELVRQDRLVITPSLAVEQPKTKLLVQQLKSLALEDVLIILEAPDEKLELA
                     ARNLPHVELVAAAAVNPVSLARHEKVLATVGAVKLLEERLQ"
     gene            3765..4088
                     /gene="rplW"
                     /locus_tag="VNZ06_13010"
     CDS             3765..4088
                     /gene="rplW"
                     /locus_tag="VNZ06_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004145338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWW21715.1"
                     /translation="MTATRKPLGKESLFNVLLAPHVTEKTSMALQERNQYAFRVRRDA
                     TRSDVRRAVELMFEVQVEGVQIVNEPGKTRRFGQRMGRTQDSKKAYVRLAAGQSIDYE
                     AQGKR"
     gene            4107..>4744
                     /gene="rplB"
                     /locus_tag="VNZ06_13015"
     CDS             4107..>4744
                     /gene="rplB"
                     /locus_tag="VNZ06_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWW21716.1"
                     /translation="MALIKLKPTSPGTRFQIRVSKSHLFKGGPYEALTVQQSKTGARN
                     HFGRITTRHVGGGSRQKYRLIDFRRDKDGIAAKVERVEYDPNRTAHIALLLYADGERR
                     YILAPKGVKQGDELRSGSESPIKAGNAMSLRHIPVGSTVHNIELKPGRGGQLARSAGG
                     SAQYTSREGIHAYIRLKSGETRKVHIDCRATLGELSNDEHNLRVYGKAGAKR"
BASE COUNT          965 a         1376 c         1583 g          820 t
ORIGIN      
        1 caatgccgta cgcaagggcg ttagctatcc gctgcgcgtc cgggcccgga tcgacgaatt
       61 tggcatgctg gatctggact tcttcaccgc gccggtcctg gcgcccgccg tccgcagcgc
      121 ggtggacggg tatctgaacg cacgcggcct ctaagaaagg gtgatttggc ccagaatatg
      181 cattgacagc ttcagacgtc gaaatcagaa tacgcggctc gtttttcgcg gaggggtacc
      241 caagcggcca acgggagcag actgtaaatc tgccggctta cgccttcgta ggttcgaatc
      301 ctaccccctc caccaggaga gagtcaggac tcgaagccgg cagacgcgct ggtcggaatt
      361 gaggacttcg gcgtcatcac taggcgggtg tagttcaatg gtagaacctc agccttccaa
      421 gctgatgacg tgggttcgat tcccactacc cgctccaaat cgattggttc gttgaagtat
      481 caccacgacc gaagctgtag ctcgcaaggc gaattgtaga gaggcgtttt aggtaccgtc
      541 cgcccacgta gctcagtcgg tagagcacgt ccttggtaag gacgaggtca ccggttcgat
      601 tccggtcgtg ggctccagtg actagtcata ttttgaggag tcgtcgtgtc gaaagagaaa
      661 tttgagcgca ccaagccgca cgtgaacgtg gggacgatcg ggcatgtgga ccatggcaag
      721 acgacgctga cggcggcgct gacgaaggtg atggccgaga cgcatggcgg cacgtttgtg
      781 gcgtacgacc agattgacaa ggcgccggag gagaaggcgc gcgggatcac gatttcaacc
      841 gcgcacgtgg agtatcagag caaggctcgc cactacgctc atgtggactg ccccgggcac
      901 gccgactacg tgaagaacat gatcaccggg gcggcgcaga tggacggggc gattctggtg
      961 gtgtcggccg cggacggtcc gatgccgcag acgcgcgagc acattctgct ggcgcgtcag
     1021 gtgggcgtgc cgcacattgt ggtgttcatg aacaaagcgg acatggtgga tgacaaggag
     1081 ctgctggaac tggtggagat ggaggtgcgc gagcttttga ccacctacaa gtttccgggt
     1141 gacaagaccc cgatcatcat cggcagtgcg ctcaaggcgc tggaagggga caagagcgag
     1201 atcggtgtgg cggcggtggt gaagctggtg gaggagatgg acaagtacat ccctgtgtcc
     1261 aagcgcgagg tggacaagcc gtttttgatg ccggtggagg atgtgttttc gatctcaggg
     1321 cgcggcacgg tggtgaccgg gcgcattgag cgcgggatcg tgaaggtcaa tgacgaggtg
     1381 gagatcattg ggctgaaggc gacgcagaag accacgtgca cgggagtgga gatgttccgc
     1441 aagctcctgg atgaggggca ggcgggggac aacgtgggag tgctgctgcg aggcaccaag
     1501 cgcgaggaag tcgagcgcgg gcaggtgctg gccaagcccg ggagcatcac gccgcacacg
     1561 cattttgagt gcgaggtgta cgtgctgacg aaagaggaag gcgggcggca cacgccgttt
     1621 ttcaaggggt atcggccgca gttttacttt cgcacgaccg atgtgacggg gatgatcgaa
     1681 ttgcctggga acaccgagat ggtgatgccc ggggacaaca tccagatgac ggtggagctg
     1741 atcgccccga tcgcgatgga gcaggggctg cgctttgcga tccgcgaggg tggtcgtacc
     1801 gtcggctccg gcgtcgtgtc caagatcctg aagtgatgac atcgttacag gcttataggt
     1861 agacgagcat ggccaaccag aacattcgca tccgattgaa ggcgtttgat catcgcctga
     1921 tcgacaactc cgccaaggaa atcgtcgata cggcacgccg taccggcgcg actgtgcgtg
     1981 gtccggtgcc gctgccgacc aagatggagc gcttcacgct gctgatctcc ccgcacgccg
     2041 acaaggacgc gcgcgatcag ctcgagctgc ggacccacaa gcgcctgatg gacatcctga
     2101 accccaccga caagaccgtg gacgccctga tgcgtctgga actgccagcc ggtgtggacg
     2161 tccagatccg cttgaactga agcgccccaa gcgcttgaac cgcgcggtct aactgctata
     2221 ctgcgcggcc tttttcacag gctggaaaca cgcttcgaac gtggctccgg cccccgccgc
     2281 gaaagcggat ttatcgggcg cggttggcct gacggttggg ccaagaccac tcgagagcta
     2341 cggcgctata tcgcgctgcc ctcctgtagt cacgcttgaa aacaaacggc actcaccggc
     2401 attcggtgcg cgcctattga gaaggactgg agtcatggct atcggtctgg tcggccgcaa
     2461 atgcggcatg acgcgcgtgt tcaccgacgg cggtgaatcg ctggcggtca cagtggtgga
     2521 agtgctgccg aaccgcgtca ctcaagtcaa atccgttgaa aaggacggct atcgtgcggt
     2581 gcaggtgacc tttggtgccc gccgcccgca gctgctgtcc aaggccgcag ccgggcactt
     2641 cgccagggcc aatgtcgaac ctggccgtgg cctggtcgaa tttcgtctag gtcccaagga
     2701 cggcgctgat ctggccgcag gcaaagagct gaaggtggac ctgttctctg aaggacagat
     2761 cgtcgatgtc accggtatca ccatcggtaa gggctatgcc ggtgcgatga aacgccacaa
     2821 ctttgccggc ggcatggcca cccacggtgc ctcgctggtg catcgcatgc cgggatcgat
     2881 cggacagcgt cagacgcccg gtcgcgtatt cccgggcaag cgcatgtcgg gacatatggg
     2941 ggtggtgcgt cgcaccaccg agaatctgaa ggtcgtcggc gtcgacggca aacgcaacct
     3001 gctgctgatt cacggctcgg ttccgggcgc cgaaggcggc caggtcatcg tgcgagcctc
     3061 ggtgaaagct gcccgcaagg cgcaccgcaa gacgatcgcg ccgagcaagg tcggggctcc
     3121 gaaggccgct gcggccgcag cccctgccaa gaagtgagcg ctatgaaact caaagtcagc
     3181 aatggcggtg ccgagattgc ggtatctgat gagaacttcg gccgcgagta caacccggcg
     3241 ctggtgtttc aggtcgtcaa tgcctacagg gcggccggcc gtgcgggcac caaggcgcag
     3301 aagacccgcg ctcaagtgcg cggtggcggt cgcaagccgc acgcccagaa gggcaccggc
     3361 tcggcacgcg caggctcgat ccgcagcccg atctgggttg gcggtggcag gaccttcgcc
     3421 gcgcagccac gtgacttctc gcagaaagtg aaccgcaaga tgtatcgcgg cgccatccgc
     3481 tccatgctct cggagctggt acgtcaggac cgactggtca tcaccccgtc gctggcggtc
     3541 gagcagccga aaaccaagct cctggtgcag cagctgaagt cactcgcgct cgaagacgtg
     3601 ctgatcattc tggaagcccc ggatgagaag ctcgaactgg ctgcgcgcaa tctgccgcac
     3661 gtcgagctgg tcgccgccgc tgcggtcaat ccggtgagtc tggcccggca cgagaaggtg
     3721 ctggcgaccg tcggtgcggt gaaactgctc gaggagcgcc tgcaatgact gcgacacgca
     3781 agccgctggg caaggagagt ctgttcaacg tgctgctcgc accgcacgtc actgagaaga
     3841 cctcgatggc gctgcaggag cgcaatcagt acgcgtttcg cgtgcgccgc gacgcgaccc
     3901 gcagcgatgt gcgccgcgcg gtcgagctga tgttcgaagt gcaggtcgag ggcgtgcaga
     3961 tcgtcaacga gccgggcaag acccggcgtt tcggccagcg catgggccgc acgcaggatt
     4021 cgaagaaggc gtatgtgcgt ctggccgccg gacagagcat cgactacgaa gcgcagggca
     4081 agcgctaatc gcgtgaagga atcatcatgg ccctgatcaa actcaaaccc acttcgcccg
     4141 gcacgcgctt ccagattcgc gtcagcaaga gccacctgtt caaaggtgga ccatatgagg
     4201 ctttgacggt tcagcagagc aagaccggcg cgcgcaatca tttcggccgc atcaccacgc
     4261 ggcacgtcgg cggcggttct cggcaaaagt atcgcctgat tgatttccgt cgcgacaagg
     4321 acggtatcgc cgccaaggtc gagcgcgttg aatacgatcc gaatcgcacc gcgcacattg
     4381 cgctgctgct gtacgcagac ggcgagcgtc gctacattct cgcgcccaag ggcgtgaagc
     4441 agggcgatga gctgcgttcg ggcagcgagt caccgatcaa ggccggtaat gcgatgtcgc
     4501 tgcgtcacat cccggtcggc tcgaccgtgc acaacatcga gctgaagcca ggtcgcggtg
     4561 gtcagctggc gcgcagcgcc ggcggctctg cgcagtacac ctcgcgcgag ggcattcacg
     4621 cctacatccg cctgaagtcc ggcgaaacgc gcaaggtgca catcgattgc cgcgccacac
     4681 tcggcgaact gagcaacgac gagcacaatc tgcgcgtata cggcaaggcc ggcgccaagc
     4741 gctg
//
LOCUS       DATMRM010000427         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3025213, whole genome shotgun sequence.
ACCESSION   DATMRM010000427 DATMRM010000000
VERSION     DATMRM010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3025213"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            330..2627
                     /locus_tag="VNZ06_13020"
     CDS             330..2627
                     /locus_tag="VNZ06_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWW21717.1"
                     /translation="MSTILLLCLLFGIAAILLLVLYFLQRRQLNALEQVSQQVQRVAI
                     GGSLRTKVDVQTDQPELAVLVTAVNHLLARVGSAAERARVPNAPISSLGDRVHEAVLI
                     HGQAIRYANPQFANLIGSRVDEVVGKRLQELVPGEFAELVADNMRRRLVGESTAERYE
                     IDLVAPGGLTSRLELSSWPIQFDGEPALLIVGVEVLPTQTVQALQAPGSRSQARRALE
                     SLSEALVTTDVDGRVDYMNPAAGRLLGVGVEDAIGSSLDDLAKVVDEADRKLLADPVR
                     QALAVGTPLNLGRRAILIQAPESAERLVELSAAPIRLETGETVGAVMLFHDVTEMRGI
                     ARQMSYQATHDALTGLVNRREFERRLLEALETAHRGDGSHVLCYLDLDRFKAINDAGG
                     HGAGDAMLRETAKILRDAVRDSDTVSRIGGDEFGMLLAGCPLEKARQIADDVCRAVNE
                     FRFVWKDKIFNVGVSVGLVEIGRDSGTIEEALAAADSACYVAKRQGGHVAVYSARDEI
                     FARQTGEIQWLQTLQSALRDSRFELYCQPIIAALAGNEEGPAMEVLVRLRDEKTGEHL
                     SAGEFVRAAERYRLMVLVDRWVVRTTLTALGHGGIALAPKRSVAINISGQTLADAQFL
                     EFVVDCFDSTGANPEQVCFEIAENAVVANPEQARRFVGVLHGMGCRFALDDFGSGLGS
                     FSHLKNLAVDYLKIDGSFFKNLARDTVNQAMVAAMIKLARSLNFKVIAEQVEDASALE
                     AARRIGVDYLQGYAVGRPEKLSMAA"
     gene            complement(2663..>3374)
                     /locus_tag="VNZ06_13025"
     CDS             complement(2663..>3374)
                     /locus_tag="VNZ06_13025"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HWW21718.1"
                     /translation="DPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHNLEAHLGEK
                     VSFSLSDPAVIIGLFIGGMVPYLFGAMAMEAVGRAAGSVVTEVRRQFREIKGIMEGTA
                     KPDYSRAVDLLTRAAIKEMVIPSVLPILVPVIVAFGMNRLMGPGAGIRALGGLLIGTI
                     VTGLFVAISMCTGGGAWDNAKKYIEEGNFGGKGSDTHKAAVTGDTVGDPYKDTAGPAI
                     NPLIKIINIVALLLVPLL"
BASE COUNT          623 a         1005 c         1103 g          643 t
ORIGIN      
        1 cgaacgccag atctcgccat gcctcaggta cgaagcgctc gcgcggtact gcacgcaagg
       61 tgtcgagcac gcgcgggtcg agaacatccc aggcgcgcag ctgctgctcg atcatttgct
      121 cgcgcgcgaa ctccgtctgc attgaactta atccagattg ggcggggacg cggcacgtta
      181 agggtttttg ccgcgcctgc caaggccggc ggcacgatat ggtgaaatga cgtagggcct
      241 tgctgatact ccgctatgct gctaatgcga gcgatggcag agcttggccc cacgccgagg
      301 aaaaagcatc gcttgtcaga acgactttga tgtcgacgat tctcctgctc tgcctgcttt
      361 tcggtattgc ggcaatactg ctattggtgc tgtattttct gcagcgccgc cagctcaatg
      421 cgcttgaaca ggtttcgcag caggtacagc gggttgccat cggtggctcg cttcgaacca
      481 aagtggatgt gcaaaccgac cagccggaac tggcggtact ggtgaccgcg gtcaaccatc
      541 tgctggcgcg ggtcggttca gccgccgaac gggcgcgtgt gccaaacgcc ccgatcagct
      601 ccctgggcga tcgggtgcac gaagcagtac tcattcacgg ccaggcgatc cgctacgcca
      661 atccgcaatt tgccaacctg atcggtagtc gggtggacga ggttgttggc aagcgtttgc
      721 aggaactggt gccgggcgaa ttcgcagaac tggtcgccga caacatgcgt cgccggcttg
      781 tgggcgaatc tactgccgaa cgctacgaga tcgacctggt cgctccaggc ggcctgacct
      841 cccggctcga gttgtcgagc tggcccatcc agtttgacgg tgagcctgcg ctgctgatcg
      901 tcggagtcga agtgctaccg acccagactg tgcaggcact gcaggcaccg ggctcgcgct
      961 cgcaagcgcg ccgcgcactc gaatcgttgt ccgaagcact ggtcaccact gacgtggacg
     1021 gacgcgttga ctatatgaat ccggccgccg ggcgcctgct gggcgtaggg gtcgaggatg
     1081 ctatcggctc ctcactcgat gatctggcaa aggtcgtgga cgaggccgat cgcaaactgc
     1141 tggccgatcc ggtgcgtcag gcgctggcgg tgggcacacc gttgaatctg ggcagacgcg
     1201 cgatattgat acaggcgccg gagagcgctg agcgactcgt ggagctttcg gcggcaccga
     1261 tccgtctgga gacaggggag acggtcggtg ccgtcatgtt gtttcacgat gtgacggaga
     1321 tgcgcggtat cgcgcgtcag atgtcctacc aggcaacgca cgatgcgctc acgggattgg
     1381 tcaatcggcg ggaattcgaa cgccgcctgc tggaggcgct ggagacagca catcgtggcg
     1441 acggttcgca cgtgctgtgc tacctggatc tcgatcgctt caaggccatc aacgacgccg
     1501 gcggtcatgg cgcaggcgat gcgatgctgc gcgagaccgc caagattctc cgcgatgcgg
     1561 tccgtgattc cgacacggtg tcacgcatcg gcggagacga gttcggcatg ctgctggcgg
     1621 gctgtccgct cgagaaggca cgacagatcg cagatgatgt ctgccgcgcg gtcaacgaat
     1681 ttcgcttcgt ctggaaggac aagattttca atgtcggggt gagcgtcggc ctggttgaaa
     1741 tcgggcgcga cagcggcacc atcgaagaag cgctggctgc ggctgattcg gcctgctacg
     1801 tggccaagcg tcagggcgga cacgtcgcgg tgtattcagc gcgcgatgag atctttgccc
     1861 ggcagaccgg tgagatccag tggctgcaga cactgcagtc ggccctgcgc gacagccgct
     1921 tcgaactcta ttgccagcca atcattgccg cgttggcggg caacgaggaa ggcccggcga
     1981 tggaagtgct ggtgcggctg cgtgatgaga agaccggcga acatctgtcc gcgggggaat
     2041 ttgttcgtgc cgccgagcgc tatcgtctga tggtgttggt ggatcggtgg gtcgttcgca
     2101 ccacgctcac ggcactggga catggcggca tcgcactggc acccaagcgc agtgtggcca
     2161 tcaatatatc gggacagacg ctggcggatg cgcagtttct ggagttcgtc gtggactgct
     2221 tcgattccac cggggccaac cccgaacagg tgtgcttcga gattgcagaa aacgctgtgg
     2281 tagccaatcc cgaacaggcg cggcgcttcg ttggggttct gcatggcatg ggctgccgct
     2341 ttgcactgga tgacttcggc agcggcctcg gctcattctc gcacctcaag aatctggcgg
     2401 tcgactacct gaagatcgac ggttccttct tcaagaatct ggcgcgcgat acggtcaatc
     2461 aggcgatggt tgccgccatg atcaaactgg cgcgcagcct gaacttcaaa gtgatcgccg
     2521 aacaggtcga ggacgcgagc gcgctggagg ccgcgcgccg catcggggtg gactatctgc
     2581 aggggtacgc cgtcggccgg cctgaaaagc tctccatggc ggcctgaagg ccccgcaaaa
     2641 gggggccgcc gttatcgcaa cgctacagca gcggtaccag cagcagcgcc acgatattga
     2701 tgatcttgat gaggggattg atcgcaggtc cagccgtatc cttgtaggga tcgccgaccg
     2761 tatcgccggt caccgcggcc ttgtgcgtat cggagccttt gccaccgaag ttaccttcct
     2821 cgatgtactt cttggcgttg tcccacgcgc cgccgccggt gcacatcgag atcgccacga
     2881 acaggccggt cacgatcgta ccgatcagca ggccgccgag cgcgcgaatc ccggcgccgg
     2941 gtcccatcag gcggttcata ccaaaggcga cgattaccgg caccaggatt ggcagcaccg
     3001 acgggatcac catctccttg atcgccgcgc gggtgagcag atccaccgcg cgcgagtagt
     3061 ccggcttggc ggtgccttcc atgatgccct tgatctcgcg gaactggcga cgcacttcag
     3121 tgactaccga gcccgcggcg cggccgacgg cttccatcgc catcgcgccg aacagatagg
     3181 gcaccatgcc gccgatgaac agaccaatga tcacggccgg atccgacagc gagaagctga
     3241 ccttttcccc gaggtgcgct tcgaggttgt gtgtgtagtc ggcgaacagc accagtgccg
     3301 ccaggccggc cgatccgatc gcgtagccct tggtgaccgc tttggtcgtg ttgccgaccg
     3361 catccagagg atcg
//
LOCUS       DATMRM010000428         5658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1499290, whole genome shotgun sequence.
ACCESSION   DATMRM010000428 DATMRM010000000
VERSION     DATMRM010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1499290"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VNZ06_13030"
     CDS             <1..433
                     /locus_tag="VNZ06_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW21719.1"
                     /translation="PTVTREKTDRRTFALVVDDSITVRRVTQRLLERNGMRVMTARDG
                     MDAVAVLAENVPDIILLDIEMPRMDGYEVAAHVRNDARLRDVPIIMITSRVGEKHRAR
                     AIELGVDDYLGKPYQESQLIDAIAPLVERRRARARASGAGR"
     gene            430..918
                     /locus_tag="VNZ06_13035"
     CDS             430..918
                     /locus_tag="VNZ06_13035"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWW21720.1"
                     /translation="MTERIAEVYSLLIPLADSRLLVPRACVAEVIGYQMPTPMESAPP
                     WYLGVVNWNARSVPLVSFEGASGLMIPPPSGRSRVVVFHALAGKAESGFIGIVSQGFP
                     QLVRVSNDVVRPDNSCSFADRVPVLCQVRMLNETPLIPDLERLESMIAEETSVMPERD
                     TG"
     gene            1065..2234
                     /locus_tag="VNZ06_13040"
     CDS             1065..2234
                     /locus_tag="VNZ06_13040"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW21721.1"
                     /translation="MIRILFIDDDSQILDGIRRSMHCMRAEWQMRFCSEPVAALNELS
                     AEPADVVVSDMRMPVMDGSEFLAEVKQRYPQTIRILLSGQAESESIIRATRSAHRYLS
                     KPCDAPTLKAAITRTANLKAVLNDGQLAAMVGSVDALPTPPKAYQELVDCIRNPNSAI
                     ADAARIIRQDVAMTAKVLKLANSGFFGLREPVQSIDRAVAFVGMEAMSALVLGQELFN
                     NKNPVTLPGFSLENLGRHSFECAAWARAVALSQQCSARVADAAFLCGVLHDVGRLVFA
                     TRVPPSAPAQRRDWLSETAMNMKTHHARVGGYLLGLWGFPEAVVESIVWHHTPSRCGD
                     MGFGLCGLLHVGDYLSHASQPHPAEAAGPELEPGYLQDRGLAEKLPEWLLTRAQK"
     gene            2231..3892
                     /locus_tag="VNZ06_13045"
     CDS             2231..3892
                     /locus_tag="VNZ06_13045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW21722.1"
                     /translation="MTDEHQIWSIGLDWLPQPIAVLNAAGMVVYCNRAWLAHRQSNLG
                     GWFVEAFSGNGLGPEARTEVAAALQLALSGADAMQSALYACGAESESHCYRVSLRRFQ
                     VKDETYVLVHHEDVTAVRRLEAELQHRIEQSHSADQTVRRVREDEVRWKLALEVNNVA
                     VWDFDALARTVVGSKRWLELWDAREGDPDVRRSGIPLPIDRIQPDELPQFLHDWYELL
                     AGMRPALEVGVNVLVLDDYRFMRLRGRVVERDAFGTALRVVGTLVDIHDARRMQMQAA
                     NASKLESIGELAAGIAHEINTPTQYVGDNVRFLGDVFGSLQQCVDELSRMTEEARDSI
                     PIERVRQCVDKADMSYLREEIPKAIAQSLEGIQRIAKIVAAMKEFSHPSQERTPTDLN
                     HAISNTITVATNEWKYVAKVETQLDSSLPQVPVIPGEFNQVILNMVVNAAHAIADTRE
                     QHPTRIGTIRIVTRRLPDWAEINISDDGCGMPKNIHEKIFDPFFTTKPVGKGTGQGLA
                     IAHNVIVKRHYGTIAVKSEAGVGTTFTIRVPLEAKEGAEPAESAA"
     gene            3905..5044
                     /locus_tag="VNZ06_13050"
     CDS             3905..5044
                     /locus_tag="VNZ06_13050"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW21723.1"
                     /translation="MAEAVLFVDDEPHLLDGIARSLRQHFSIHTATSGAAGLRLLQSA
                     GPFAVVVSDMRMPEMDGVQFLSKVAALAPDTVRVILTGQADLAQTIAAVNEGHIFRFL
                     NKPCTTQFLQGAVENAIAQHRLITAEKVLLEQTLTGAVNMLLEILSAILPSAHGRARR
                     LQRYIVALARALALPVDWQWPLAASVSQVGCISIPKDILAKVEAEQPLSDDEQRLFDS
                     HPRMAGKMLEAIPRLEGVAAIVTAQNMPLSQAETFARLSQLDARTAGRILLRAVIEYD
                     RYVVEGHSAAAAAQAVRASAAGLPAEVLETLPGMPIARREYILRSVRLIDLAPGMLLD
                     EALVTLKGVCLVPAGHEVTPTLLQRLCGIDAGLQVREPFRVQVPT"
     gene            5056..5649
                     /locus_tag="VNZ06_13055"
     CDS             5056..5649
                     /locus_tag="VNZ06_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW21724.1"
                     /translation="MTSNRATILLVDDESHVTDALSRHFPRERFEVLSARSAADAYEI
                     LDRRRVDVVISDERMPGEAGSEFLARVRQLHPATIRMILSGQASLDAAIRAINEGEVY
                     RFFLKPCNPVDLLFTVQRALEHRRLAERSRALLAEYRKQAALLNVVERMQPGLLKFEQ
                     DESGAIIVEDDSTDSVEDLLREIEQSIAARHPEYDHA"
BASE COUNT         1068 a         1688 c         1839 g         1063 t
ORIGIN      
        1 gccgaccgtg acccgtgaga agaccgaccg gcgcacgttt gccctggtcg tcgatgattc
       61 gatcaccgtg cgtcgggtga cgcagcgtct gctcgaacgc aacggcatgc gtgtcatgac
      121 cgcgcgcgac ggtatggatg ccgtggcggt actggcggaa aacgtgccgg acatcattct
      181 gctcgacatc gagatgccgc gtatggacgg ctatgaagtc gcagcgcacg tgcgcaacga
      241 tgcccgactg cgcgacgtgc cgatcatcat gatcacctcg cgcgtcggcg agaagcatcg
      301 cgctcgcgca atcgagctgg gtgtggatga ctatctgggc aagccctatc aggagagtca
      361 gctcatcgat gcgatcgcac cgttggttga acgccggcgt gcccgcgcgc gtgcctcggg
      421 ggccggacga tgaccgagcg gatcgccgag gtctacagcc tgctgatccc gctggcggac
      481 tcccggctgc tggtgccgcg cgcgtgtgtc gccgaggtca tcggctatca gatgccgacc
      541 ccaatggaga gtgctccgcc ctggtatctg ggcgtggtca attggaatgc gcgcagcgtg
      601 ccgctggtgt cgttcgaagg tgccagcgga ctgatgattc cgccccccag cggtcgctca
      661 cgcgtcgtgg tgtttcatgc gctcgctggc aaagccgagt cgggcttcat cggtatcgtc
      721 tcgcagggat tcccgcaatt ggtgcgcgtc agcaatgacg tggtccgccc ggacaattcg
      781 tgcagttttg cggaccgtgt gccggtgctg tgtcaggtgc gcatgctcaa tgaaacccct
      841 ctgattcctg atctggagcg actcgagtcc atgatcgcgg aagaaacgtc ggtgatgccc
      901 gagcgcgata ccggctgagc gcgcaatcgg aaatcgatcg gcaatctcca ccacgcaatt
      961 gttcgagtgt catctctgag gatgtgacgc gcttcgcgta accagctcaa gcgctcgcgc
     1021 ccggagccga tgcaccggaa tatctgccgg aaggtagaac tgccgtgatt cgcatcttgt
     1081 tcatcgacga tgatagtcag attctggacg gaatccgacg cagcatgcat tgcatgcgcg
     1141 ccgaatggca gatgcgtttt tgcagcgaac ccgtcgctgc gctgaatgaa ctgtccgccg
     1201 agcccgctga cgtggtcgtt tccgacatgc gcatgccggt gatggacggt tcagagtttc
     1261 tggccgaggt gaagcagcgc tatccgcaga ccatacgcat actcctgtcc ggacaggccg
     1321 agtccgagtc gatcattcgg gccacgcgca gtgcccaccg ctacctgtcg aaaccctgcg
     1381 acgcaccaac actgaaggcg gccatcacgc gcacagcgaa tctgaaggcg gttctgaatg
     1441 acggtcagct ggccgccatg gtaggcagcg ttgacgcgct gccgacgccg ccaaaggcct
     1501 atcaggagct ggtggactgc atccgcaacc caaactcggc gattgccgat gcggcccgca
     1561 tcatccgcca ggacgtggcg atgacggcca aagtcctgaa gcttgccaac tccggattct
     1621 tcggccttcg cgagccggtg cagagtattg accgggccgt tgccttcgtc ggtatggagg
     1681 ccatgtccgc gctggttctt ggacaggagc tgttcaacaa caagaacccg gtgacgctgc
     1741 cgggattcag tctcgagaac ctcggtcgtc attcattcga gtgtgcggcc tgggcgcgcg
     1801 cggtagcgct ctcccagcag tgcagcgcac gggtcgccga tgccgcgttt ctgtgtggcg
     1861 tgctgcatga cgtgggccgg ctcgtgttcg ccacccgcgt gccgccgtcc gcgccggcgc
     1921 agcgccggga ttggctgtcc gagactgcga tgaacatgaa gacccatcac gccagagtcg
     1981 gcggctactt gctagggttg tggggttttc cggaggcggt cgtggagtcc atcgtgtggc
     2041 atcacacgcc cagccggtgc ggtgacatgg ggttcggtct gtgtggtctg ctgcacgttg
     2101 gcgactatct gtcgcacgca agtcagccac atcctgcgga ggcggcaggc ccggaactcg
     2161 aaccaggtta cctgcaggac cgggggttgg ctgaaaaact tcctgaatgg ttgctgacgc
     2221 gagcgcaaaa gtgactgacg aacaccaaat ctggtcgatc gggctcgact ggctgccgca
     2281 gccgattgcg gtattgaacg ccgccggcat ggtggtgtat tgcaatcgcg catggctcgc
     2341 gcatcgccag tccaatctgg gtggctggtt cgtcgaagcc ttttcgggca acggcctggg
     2401 gcccgaagct cgcactgagg tcgctgccgc cctgcagctc gcgctttccg gagccgacgc
     2461 gatgcaatcg gcgctctacg cctgcggcgc tgagtccgaa tcgcactgct accgcgtgag
     2521 tctgcgccgc tttcaggtga aggatgagac ctacgtgctg gtacaccacg aggacgtcac
     2581 cgcggtgcgc cggctggaag ccgaactgca acaccgaatc gagcaatccc acagcgccga
     2641 tcagacggtg cggcgggtgc gcgaggacga ggtgcgctgg aagctggcgc tggaagtcaa
     2701 caacgtcgcg gtatgggatt tcgacgcact cgcacgaacc gtggtcggct ccaaacgctg
     2761 gctcgagctc tgggatgcgc gcgagggcga tccggacgtg cggcgcagtg gcattccgct
     2821 gcccattgat cgcattcaac cggacgaatt gccccagttc ctgcacgatt ggtacgaact
     2881 gctggccgga atgcggccag cgctggaagt gggcgtaaat gtgctggtgc tggatgacta
     2941 tcgtttcatg cgtctgcgcg ggcgggtcgt ggagcgcgat gcattcggta ccgcgctgcg
     3001 agtggtcggc actttggtcg acattcacga cgcccgccgg atgcagatgc aggccgccaa
     3061 tgcgagcaag ctggaatcca ttggcgaact ggcggcgggc attgcccatg aaatcaatac
     3121 accgacacag tacgtgggcg acaacgtgcg cttcctgggc gatgtctttg gatctctgca
     3181 gcagtgcgtc gatgagttga gccgaatgac tgaggaagcg cgcgacagca ttccgatcga
     3241 gcgcgttcgc cagtgcgtcg acaaggccga tatgtcctac ctgcgcgagg agattcccaa
     3301 agcgatcgcg cagtcgctcg aaggtattca gagaatcgcc aagatcgtcg cggcgatgaa
     3361 ggagttctca caccccagtc aggagcgcac gcccaccgat ctgaatcacg cgatcagcaa
     3421 cacgatcacc gtggcgacca atgagtggaa gtacgtggcg aaggtggaaa cgcagctgga
     3481 ttcgtcgctg ccgcaggtgc cggtcatccc cggagagttc aaccaggtga ttctgaatat
     3541 ggtggtcaac gccgcgcacg cgatcgctga cacgcgcgag cagcatccaa cgcgcatcgg
     3601 tacgatccgc atcgttacgc gacgattgcc cgactgggcg gagatcaata tttcggacga
     3661 cggctgcggc atgccgaaga acattcacga aaagatcttc gatccgttct tcaccaccaa
     3721 accggtgggc aagggtaccg gacagggcct ggcgatcgcg cataatgtca tcgtaaaacg
     3781 acattacggt acgatcgccg tgaagagcga agcgggcgtg ggtacgacct ttacgatccg
     3841 tgtgccgctc gaagccaaag aaggcgcaga gcccgcggag agcgccgcgt aaccggcggc
     3901 aaccatggca gaggcggtgc ttttcgttga cgatgagccg cacctgctcg acgggattgc
     3961 ccgctcgttg cgacagcatt tctcaattca caccgcgacc agcggggcag caggtctgcg
     4021 cctgcttcag agcgccggcc cgttcgcggt cgtcgtctcc gatatgcgta tgccggagat
     4081 ggatggtgtt cagtttctgt ccaaggtcgc ggcgctggca ccagacaccg tgcgcgtgat
     4141 cctgaccggg caggccgatc tcgcccagac aatcgcggcg gtcaacgaag ggcacatttt
     4201 ccgattcctg aacaagccgt gcacgacgca gttcctgcag ggagcggtgg agaacgcgat
     4261 tgcgcagcac cgactgatta ccgcagagaa agtactgctc gagcagacgc tcaccggagc
     4321 ggtgaacatg ctgctggaaa tcctaagcgc cattctgccg tcggcccatg ggcgggcgcg
     4381 gcgcctgcag cgctacatcg tcgcgctggc cagagcgctg gcgctgccgg tggactggca
     4441 atggccgctg gcggcctcgg tttcgcaggt cggttgcatt tcgataccga aggacattct
     4501 ggcgaaagtc gaagccgagc agccgctgag tgatgacgag cagcgcttgt tcgactcaca
     4561 tccgcgcatg gccggaaaga tgctcgaggc gatcccgcga ctggaaggcg tggcggccat
     4621 cgtgacggca cagaacatgc cgttgagcca agcggagacc tttgcgcggc tgtctcagct
     4681 cgacgcccga acggccggca ggattctgct gcgagcggtc atcgagtacg accggtacgt
     4741 tgtcgaaggt cacagcgccg cagcggccgc ccaggccgtg cgtgcctccg ctgccggatt
     4801 accggctgaa gtgctcgaga cattgccagg tatgccgatc gcacggcgcg aatacatcct
     4861 gcgcagcgtg cgcctgattg atctcgcccc cggcatgctg ctcgatgagg cattggtcac
     4921 actcaaaggc gtgtgcctgg tgccggctgg gcatgaagtc acacccactt tgttgcagcg
     4981 gctgtgcggt atcgacgcgg gcttgcaggt tcgagagccg tttcgggttc aggttccgac
     5041 atagagggca cgatcatgac gtccaacagg gccactattc tgctcgtcga tgatgagtca
     5101 cacgtaaccg atgcgctcag tcggcacttt ccaagggagc gttttgaggt gctgagcgcc
     5161 cggtccgccg cggatgcgta tgagattctc gatcgacggc gcgtcgacgt ggtgatatcg
     5221 gatgagcgca tgccgggcga ggcgggttcc gagttcctgg cgagagtgcg gcaactgcat
     5281 ccggccacga tccgcatgat cctgtccgga caggcgagtc tggacgccgc tattcgtgcg
     5341 atcaacgaag gagaggtgta ccgattcttt ctcaaaccgt gcaatccggt cgatctgctg
     5401 ttcacagtgc agcgtgcgct ggagcatcgt cgtctggccg aacgcagtcg cgcactgctg
     5461 gccgagtatc gcaagcaggc ggctctgctc aatgtcgtgg agcgcatgca acctgggctg
     5521 ctcaaattcg aacaggacga atccggcgcc atcatcgtgg aggatgacag caccgattcg
     5581 gtggaagacc tgttgcgcga gatcgaacaa tccatcgccg cccgccaccc cgaatacgat
     5641 cacgcttagc ctttgagc
//
LOCUS       DATMRM010000429         3026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1832534, whole genome shotgun sequence.
ACCESSION   DATMRM010000429 DATMRM010000000
VERSION     DATMRM010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832534"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VNZ06_13060"
     CDS             complement(<1..574)
                     /locus_tag="VNZ06_13060"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HWW21725.1"
                     /translation="MACTCLLLFACASQAPRPRSDIRPAASPEALTNLRRQDALWLER
                     VGFGVDSVMVKDYRRLGRTRWLDQQLGAHDETLPPAIAAQLQNMEVSHLDAQHLLADV
                     QARQKAINAMADGPDRESARRTLNDSGNRAAYDSIRRQLLRAVYSPNQLREQMVWFWL
                     NHFSVYQYKSNLRWLIGDYEETAIRPYALGH"
     gene            761..1159
                     /locus_tag="VNZ06_13065"
     CDS             761..1159
                     /locus_tag="VNZ06_13065"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWW21726.1"
                     /translation="MKGIIRHTGGCHCGRVRFEVQAPAHIEASDCNCSICSKAGYLHL
                     TVPKSRFRLLSGEDSLTTYQFNTRTAKHLFCCVCGIKSFYVPRSHPDGFSVNARCLDA
                     GTVTGMSVKPVDGRNWEQVYPKGRGAPLEE"
     gene            1179..2486
                     /locus_tag="VNZ06_13070"
     CDS             1179..2486
                     /locus_tag="VNZ06_13070"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HWW21727.1"
                     /translation="MHPHDLLFAMFRAALDAASPSRCVPSHLPERPDGRVVVVGAGKA
                     AAEMARAVEERWGDAVTGLVVTRYGHRSPCQHIEVVEAGHPNPDAASYNAAVRIRNLV
                     SGLTADDLVLCLWSGGGSSLLSLPAQGITLADKRGINQQLLRSGAGIAEINCIRKHLS
                     AIKGGRLALLAQPAQVLSLIISDVPGDDVSVVASGPTMADSTTREQALEILERYDLNV
                     PAHVMEWLRSEGAETPKPGDERLARVRNVLIATPLASLMAAAEYARAQGVRPLILGDA
                     IEGEARECARVHAGIAISCATHGHPLPAPCVLISGGETTVTVRGGGRGGRNAEFLLAL
                     ALALQAHPQVWAIACDTDGIDGTEDNAGARIAPDTLDRASAWGVNAVTSLEDNDAYRV
                     FQVLNDLIVTGPTRTNVNDFRAVLVERGSVSGAGVQLALPSAD"
     gene            complement(2463..>3026)
                     /locus_tag="VNZ06_13075"
     CDS             complement(2463..>3026)
                     /locus_tag="VNZ06_13075"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HWW21728.1"
                     /translation="TYQAASGAGAAAMQELREATRASLAGEAFEPRVLRHPYAFNLFS
                     HDASVDPITGSNDEETKVGRELRKIMAAPDLRVSVTCIRVPVLRAHAIALNVEFERTV
                     TPESARELLQRAAGVRVVDDIERNHFPMPNEASGQGDVFVGRIRRDSSDPSGHSLSMF
                     VAGDQLLKGAALNAVQIAERLVGARQG"
BASE COUNT          557 a          912 c          976 g          581 t
ORIGIN      
        1 agtgcccgag cgcataggga cggatggcgg tttcttcgta gtcgccgatc aaccagcgaa
       61 gattgctctt gtattgatac acgctgaagt gattgagcca gaaccagacc atctgctcgc
      121 gcagctgatt gggcgagtag accgcgcgca ggagttggcg tcggatgctg tcgtaggcgg
      181 cgcgattacc actgtcgttg agcgtcctgc gcgccgattc gcgatcaggc ccgtcggcca
      241 tcgcattgat tgccttctgg cgcgcctgaa cgtcggcgag caggtgctgc gcgtcgaggt
      301 gcgagacttc catattctgg agttgcgcgg caatcgcggg cggcaaggtc tcgtcgtgcg
      361 cgcccagctg ctgatcgagc catcgcgttc tgccgaggcg gcgatagtcc ttgaccatca
      421 cactgtcaac accgaagccg actctttcga gccacaacgc atcctgtcgt cgcagattgg
      481 tgagagcctc cgggctggct gccggtcgga tgtcgctgcg cggccgtggc gcctgagagg
      541 cgcaggcaaa caacagcagg caggtgcagg ccacgagacc caagcgagtc gctgtgacag
      601 agcctagttt cgatggttgg tccaccatgg cagtcccgtg cgcatccggc ggcggtcctg
      661 aaactcaacg agccggcgag gtgtgcgttg acatgtgtcg caggcggcag cgtaaacttt
      721 gcaacttgag acagcttata cgtcaagtat cggagatctt atgaagggga tcatccgcca
      781 taccggcggc tgccactgcg gaagggttcg gtttgaggtg caggcgccgg cgcacatcga
      841 ggcatcggac tgcaattgtt caatatgctc caaggcgggc tacctgcatc tgaccgtgcc
      901 gaagtcacgg tttcgattgc tgtccggcga agattctctg accacctatc agttcaacac
      961 gcgcaccgcc aaacatctct tctgctgcgt atgcggcatc aaatcgttct acgttcctcg
     1021 ttcgcaccct gacggcttca gcgtcaacgc ccgatgtctg gatgccggta ccgtcaccgg
     1081 catgagcgtc aaaccggtgg acggaagaaa ctgggagcaa gtctatccga agggtagagg
     1141 cgcgccgctg gaagagtagc cgcacgccgc ttcctcgcat gcatccgcat gatttgctgt
     1201 ttgcgatgtt tcgcgccgcg ctggacgctg cgagtccctc acgctgcgtg ccttcgcatc
     1261 tgcccgaacg acccgatggt cgagttgtcg tcgtcggtgc gggtaaagcc gcagcggaaa
     1321 tggcgcgggc ggttgaagag cggtggggcg atgctgtcac aggactggtc gtcacacgtt
     1381 acggtcaccg ctcgccctgc cagcacatcg aagtggtcga agcgggccat ccgaatcccg
     1441 atgccgcaag ctataatgcc gccgtacgca ttcgcaatct ggtcagcggg ctgacagccg
     1501 acgacctggt gctgtgtctg tggtccggcg gcggatcatc actgctgtca ctgcccgccc
     1561 aaggcatcac gctcgctgac aaacgaggga tcaatcagca gttgctgcgc agcggtgcgg
     1621 gcatcgcgga gatcaactgc atccgcaagc atctgtcggc gatcaaggga ggtcggttgg
     1681 cgttgctggc acagccggca caggtgctca gtctgatcat ctcggatgtt ccgggtgatg
     1741 acgtatcggt agtcgcttca ggacccacga tggccgattc gactacgcgt gagcaagcgc
     1801 tggaaatact ggagcgctac gacctgaatg tgcccgcgca tgtcatggaa tggttgcgat
     1861 cggaaggggc cgagacgcca aagcccggtg acgagcggct cgcgcgagtg cgcaatgtct
     1921 tgattgcgac acctctggca tcgctgatgg ccgcggcgga atacgcgcgt gcccagggtg
     1981 tgcgtcctct catccttggc gatgcgatcg aaggggaagc gcgcgaatgt gcaagagtgc
     2041 atgcgggtat cgcgatcagt tgtgcaacac atgggcatcc gctgcccgcg ccctgcgtgc
     2101 tgatttccgg tggcgagacc accgttaccg tgcgcggagg cggccgtggc ggacgtaacg
     2161 ccgaattcct gctggcgctg gcgctggcat tgcaggcaca tccacaggtg tgggcgatcg
     2221 cttgcgatac cgatggcatc gatggcaccg aagacaatgc cggcgcgcgc atcgctccgg
     2281 acaccctgga cagggcgagc gcctggggtg tcaacgcggt gacctcgttg gaggacaacg
     2341 atgcctaccg cgtgttccag gtgctcaacg atttgatcgt gaccggtccg acgcgcacca
     2401 acgtcaatga ctttcgcgct gttctcgttg agcgtggatc ggtgtctggg gcaggggtgc
     2461 agctagcctt gccgagcgcc gactagccgc tccgcaatct gcaccgcgtt caacgcggca
     2521 cccttcaaca gctgatcgcc ggcaacgaac atcgataacg aatgtcctga cggatcgctg
     2581 ctgtcgcgac ggatgcgccc cacgaacaca tcgccctgac cgctggcctc attcggcatc
     2641 ggaaaatgat tgcgctcgat atcatcgacg acccgcacac ctgcggcgcg ctgcaacagc
     2701 tcgcgcgcgg actcgggagt cacggtgcgc tcgaactcca cgttcagtgc gatcgcatgc
     2761 gcgcgcagca ccggcacacg gatgcaggtg acgctcacgc gcagatccgg cgccgccatg
     2821 atcttgcgca gctcgcggcc gactttggtc tcctcatcgt tcgagccggt gatcggatcg
     2881 acggacgcgt catgactgaa aagattgaac gcgtacggat gtcgcaatac gcgcggctcg
     2941 aatgcttcgc ctgcgagcga tgcgcgtgtg gcctcgcgta gctcctgcat cgctgcagcg
     3001 ccggcgccgg aggccgcctg gtaggt
//
LOCUS       DATMRM010000430         3537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1833157, whole genome shotgun sequence.
ACCESSION   DATMRM010000430 DATMRM010000000
VERSION     DATMRM010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833157"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..1167)
                     /gene="pip"
                     /locus_tag="VNZ06_13080"
     CDS             complement(178..1167)
                     /gene="pip"
                     /locus_tag="VNZ06_13080"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HWW21729.1"
                     /translation="MPRSVRRVAGTLYPAIRPYRKAYLRVSPLHELYYEESGNPRGKA
                     VVFLHGGPGAATNPAMRRFFDPRRYRIVLFDQRGCGASRPHAELRENSTWELVSDIEQ
                     LRTHLGIERWMVFGGSWGSTLALAYAQRHPEYVTELVLRGIFLLRRSELEWFYQSPQG
                     AASLFPDLWEHYVAPVPPEERGDMMRAYYRRLTSDDPKTRASAARAWSMWEGATSYLR
                     TNSSYLSKFEDPEYAAAFARIEAHYFVNRGFMDSDDQLLRDAHRLRDIRGVIVQGRYD
                     LVCPMRSAWDLHRAWPQAQLRIVPDAGHSAMETGITRELVGATDRLARRGGKG"
     gene            complement(1172..1513)
                     /locus_tag="VNZ06_13085"
     CDS             complement(1172..1513)
                     /locus_tag="VNZ06_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005695575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HWW21730.1"
                     /translation="MPTLFERIIQGELPAKFVYKDDRVVAFRDIHPRAPTHVLIVPNK
                     PIPTANEITAEDESLVGHMVAVAAQIARSEGIADDGYRLIVNCNRNAGMEVYHLHLHL
                     LGGKPLGPMVS"
     gene            1528..2556
                     /gene="holA"
                     /locus_tag="VNZ06_13090"
     CDS             1528..2556
                     /gene="holA"
                     /locus_tag="VNZ06_13090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HWW21731.1"
                     /translation="MDAGDRVSLRYRDDDAQQHEGAHQVRLSLEALGTHLAQTLLPIY
                     LVSGDEPLLVGEAGDAIRARARIAGFAERQVFFIERSAAVWEQISQEAQALSLFASSR
                     VVEIRMPGGKPGTAGATALKRLFEAARSDLLVLIITGELDRDAQGADWVYAAQQRGAW
                     LPIRTVERSRLPQWLQSRFAAQGLKATDQAITLLAERSEGNLLAARQEIDKLALLLPP
                     GATVSVAEVEASSADSARFDVFQLTDAIRSADPARALRVLSGLSAEGSEPPLLLWALS
                     RELKGMQQRAGAASRIAPARLIARAARADRMAKGLSIGDAWDELALLVVEMTGKRTLP
                     LVRTGVEP"
     gene            2606..3253
                     /gene="nadD"
                     /locus_tag="VNZ06_13095"
     CDS             2606..3253
                     /gene="nadD"
                     /locus_tag="VNZ06_13095"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000839.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HWW21732.1"
                     /translation="MAEPIGLFGGTFDPIHFGHLRTAFELLQSLKLSQVRFLPTGDPP
                     HREAPLANGQLRFQMVSAAVVGQPGFTVDDREIKREGVSYTIDTLTELRQENPQRSLC
                     MLLGMDAFLGMPTWRRWREIFDLCHVVVAHRPGWKAPVTGPLGEVMVDRGTGSVRDLH
                     ASPAGKIYVHGVTQLEIASTDLRALIMSGRDLRYLVPDAVRDLILRSGCYAARNA"
     gene            3274..>3537
                     /gene="rsfS"
                     /locus_tag="VNZ06_13100"
     CDS             3274..>3537
                     /gene="rsfS"
                     /locus_tag="VNZ06_13100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HWW21733.1"
                     /translation="MTTSARPRRAPRRNSVLEKLVLTALDEMKAVNIKVLDVRGMTDI
                     ADAMIVASGTSDRHVRAIADRVIEKAKIEGHRPLGVEGQRDGEW"
BASE COUNT          664 a         1080 c         1171 g          622 t
ORIGIN      
        1 cgatgactac cggtgaggtg tagcttgcgt agaaacgcag caaggccgca tgcgaacggt
       61 cggcatcgaa gcccaggaac gggatactgt gatcgatgaa atattgcgcc agtatccgcg
      121 cgaccagcga tttgccgaca ccacctttct cgccaccaac cagatgaatc gtcgccatca
      181 gcctttacct cctcgacgcg ccaagcgatc ggtcgcgccg actagttccc gcgtaatgcc
      241 tgtttccatc gccgagtgac cggcgtccgg tacgatgcgc agctgcgcct gcggccacgc
      301 ccgatgcaga tcccaggcgc tgcgcatcgg gcacacgagg tcgtaccggc cctgcacgat
      361 gacgccacgg atgtcgcgca gacggtgtgc gtcgcgcagc agttgatcgt cgctgtccat
      421 gaagccccga ttcacgaagt agtgagcttc gatgcgcgcg aatgccgcgg cgtactcggg
      481 atcttcgaac ttcgacaggt agctcgagtt ggtgcgcagg taactggtcg caccttccca
      541 catcgaccag gcgcgcgcgg cactcgcacg cgtcttcgga tcgtcgctgg tcaggcgtcg
      601 gtaataggcc cgcatcatgt cgccgcgctc ctcgggcggg acgggtgcga cgtagtgctc
      661 ccacagatcg ggaaacagcg aggcggcacc ctgcgggctc tgatagaacc attcgagctc
      721 cgagcgccgc aacagaaaga ttccgcgcag taccagctcg gtaacgtact cgggatgccg
      781 ttgcgcgtag gcaagcgcca gcgtcgagcc ccacgagccg ccgaacacca tccagcgctc
      841 gatgccgaga tgtgtccgca gctgttcaat atcggacacc agctcccagg tgctgttctc
      901 gcgcagctcg gcatgcgggc ggctggcacc gcatccgcgc tgatcaaaca gcacgatgcg
      961 atagcgccgg ggatcgaaga accggcgcat ggcggggttg gtggccgcgc ccggtccgcc
     1021 gtgcaggaat accaccgctt tgccgcgtgg attgccgctt tcctcgtaat acagttcgtg
     1081 cagcggcgag acgcgcagat acgctttgcg atagggccgg atggcaggat agagcgttcc
     1141 cgcgacacgt cgaacggagc gcggcacggg ttcaactgac catggggccg agcggcttgc
     1201 cgcccagcag atgtaggtgc aggtggtaga cctccatacc ggcgttcctg ttgcagttga
     1261 caatcaggcg gtagccgtca tcggcaatgc cctcgctgcg cgcgatctgt gccgcgaccg
     1321 ccaccatgtg gccgaccagc gactcgtcct cggcagtgat ttcattggct gtaggtatgg
     1381 gcttgttcgg gacaatgaga acgtgagtcg gagcgcgcgg atgaatgtcg cgaaacgcca
     1441 caacgcgatc gtccttgtaa acaaatttgg ccgggagttc gccctggatg atgcgctcga
     1501 aaagtgtcgg catgattgcg acgctcgatg gatgcgggag atcgggtcag cttaagatat
     1561 cgcgatgacg atgcacaaca gcacgagggt gcgcaccagg tgagactgtc gctcgaggct
     1621 ctgggcaccc atttggcgca gacactgctg ccgatctatc tggtgtcggg cgacgagcca
     1681 ttgctggtcg gagaggctgg cgacgcaatc cgggcacggg cgcgcattgc aggatttgca
     1741 gagcgccagg tattttttat cgagcgcagc gccgccgttt gggagcagat cagccaggaa
     1801 gcccaggcgc tgtcactgtt tgccagttcg cgggtcgtcg aaatccgtat gccaggcggg
     1861 aaaccgggca ccgccggcgc caccgcgctg aagcgactgt tcgaggcggc ccgttccgat
     1921 ctgctggtgt tgatcatcac cggagagctg gatcgggacg cacagggcgc cgactgggtc
     1981 tatgcagcgc aacagcgcgg tgcctggctg cccatccgca ccgtggaacg atccaggctg
     2041 ccgcagtggt tgcagtcgcg ctttgccgcg cagggcctga aggcgaccga ccaggcgatc
     2101 acgctgttgg cggaacgcag cgagggcaat ctgctcgcgg cgcgccagga gatcgacaag
     2161 ctcgcgttgc tactgccgcc gggcgcgacg gtatccgtgg ccgaggttga ggccagcagt
     2221 gccgacagcg cgcgatttga tgtgtttcaa ttgacggatg cgatacgcag tgcggacccg
     2281 gcacgggcac tgcgagtctt aagcgggctg agcgcggagg gcagtgagcc gccgctgctc
     2341 ttgtgggcac tttcccgcga actcaagggg atgcagcaac gcgcaggtgc cgcgtcgcgg
     2401 atagcgcctg cgcgcctgat cgcacgcgcg gcgcgcgccg accgcatggc caagggactg
     2461 agcatcggcg atgcctggga cgaactggcc ctgctggtgg tcgaaatgac tggcaaacgc
     2521 accctgccgc tggtgcgcac gggcgtggag ccataaggcg cgcaaacttg cactgcgccg
     2581 cagccgccac cacactggcg atgacatggc agaaccgata ggcctattcg gcgggacctt
     2641 tgacccgatt catttcggtc atctgcggac cgcgttcgag ctgctgcagt ccttgaaact
     2701 gtcgcaggtg cggtttcttc cgaccgggga tcctccgcat cgcgaagcgc cgctggcgaa
     2761 cgggcaattg cgtttccaga tggtcagcgc tgcggtggtc ggccagcccg gattcacggt
     2821 ggatgaccgc gagatcaagc gcgaaggcgt gtcatatacg attgatacgc tcaccgagtt
     2881 gagacaagag aatccgcaac gctcgctgtg catgctgctc ggaatggatg cctttctggg
     2941 catgccaacg tggcggcggt ggcgcgaaat cttcgaccta tgccacgtcg tggtcgcgca
     3001 tcggccgggc tggaaagccc ctgtgaccgg cccgctcggc gaggtgatgg tggatcgagg
     3061 aaccgggagt gtgcgcgatc tgcacgcctc gccagccggc aaaatctatg tccatggcgt
     3121 cacgcaactg gagatcgcat cgaccgacct gcgcgcgctg attatgtcag ggcgggatct
     3181 gcgttacctg gtacccgacg ccgtccgcga tctcattctg cgcagcgggt gctatgctgc
     3241 gcgtaacgct tgaagggagg cacacctaac cgcatgacaa catccgctcg cccgcgccgc
     3301 gctccgcgac gcaactccgt gctcgagaaa ctcgtgctca cggcgctgga tgagatgaag
     3361 gcagtcaaca tcaaagtact cgatgtgcgg ggaatgaccg acatcgccga cgccatgatc
     3421 gtggcctcgg gaacttccga ccgtcatgtg cgcgcgatcg ccgatcgcgt gatcgaaaaa
     3481 gccaagattg aaggccaccg gccgctgggt gtggaaggcc agcgcgacgg cgaatgg
//
LOCUS       DATMRM010000431         5198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3643938, whole genome shotgun sequence.
ACCESSION   DATMRM010000431 DATMRM010000000
VERSION     DATMRM010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3643938"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..840)
                     /locus_tag="VNZ06_13105"
     CDS             complement(103..840)
                     /locus_tag="VNZ06_13105"
                     /inference="COORDINATES: protein motif:HMM:NF005489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWW21734.1"
                     /translation="MQRILIVGATSAIAEATAREFAARGDALFLAGRNAPLLQTIADD
                     LRLRGASIAGTVVIDVRELERLPALIASGVESLGGLDQALIAHGTLSDQNACEDSIEA
                     LREEFTVNALSVMVLCLALARVFAARGAGVLAVISSVAGDRGRHSNYAYGAAKASVTA
                     FLSGLGQQLYARGVRVLTIKPGFVDTPMTAAFPKGALWAKPARVAHDIRRAMDRGSVM
                     IYTPWFWRAIMFIVRSVPETLFRRRRG"
     gene            complement(825..2138)
                     /locus_tag="VNZ06_13110"
     CDS             complement(825..2138)
                     /locus_tag="VNZ06_13110"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWW21735.1"
                     /translation="MALGDSWGRWPRHNQQIVALRSRFEPLPDFAPLLPYGNGRSYGD
                     VCLNDGGTLLSTRSLDHLIAFDAATGVLECEAGVLLSEIIELLAPRHWMLPVVPGTAL
                     VTVGGAIANDVHGKNHHRVGTFCHHLLDFELLRSNGEFLICSPHQNTELFRATIGGLG
                     LTGLIRSARLQLRTIDGPWIEGETQRFANMEEFLALSAASDDEHEYTVAWFDSTAPAQ
                     QRGRGVFMRANHCAITDPAPIRQGQVRVPLTPPFSVVSQFTTRILNGLYYRRSATRPK
                     QRQWLQQRFLFPLDRILDWNRVYGPRGFFQYQCVLPAQGATDAFKTMLGLLERHRQGS
                     FVSVLKKFGSAPSLGLLSFARPGVTVAMDFPNRGADTLRLLASFDSVTEQAGGAVYPA
                     KDARMSAASFQRYFPAWEAFRQFVDPRFSSSFWRRVTGQPSCSAS"
     gene            complement(2147..3541)
                     /locus_tag="VNZ06_13115"
     CDS             complement(2147..3541)
                     /locus_tag="VNZ06_13115"
                     /inference="COORDINATES: protein motif:HMM:NF006088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWW21736.1"
                     /translation="MTDAAIPLCIDFDGTLTANPLQLELLLLLAKQSPVRALAARPTD
                     RPDSTTDVDVLGLPLRHDLIHWLQNERRSGRRLVLVVNGDRRIADQVTTPLGLFDEYA
                     WVDPRGGSPAERKRSALVARFGEQGFDYAGSQRSDQIVWKAARRAVVVGDTALANEVR
                     RVSDVEQVFPRSTPSISIWMRAVRLHQWVKNTLVFLPALLAHTIFQATVLRSCALAFI
                     AFGLCASSVYLTNDLFDLAADRAHPHKRTRPLACGEISPAAGAWASLLLLLGAAAVSA
                     ALDLRFALALVGYYLLTCAYSVRLKRVALLDVMVLATLYTIRIIAGAAASNVPVSFWL
                     LAFSVFMFMSLAFIKRYAELESTRRTERPAGRGRDYSAEDMPAIMSLGSASGLCAIVV
                     TALYINSPESQAIYRYHHVLWLICPLMLFWISRAWMLAARGRLHDDPLVFAVQDGVSL
                     VTLGLLVVIVYLAI"
     gene            complement(3718..3867)
                     /locus_tag="VNZ06_13120"
     CDS             complement(3718..3867)
                     /locus_tag="VNZ06_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21737.1"
                     /translation="MTNKISIKTVGVQTYVISELAAGNWYFTLTSVNSAGAESIASSA
                     VEATL"
     gene            3962..4519
                     /locus_tag="VNZ06_13125"
     CDS             3962..4519
                     /locus_tag="VNZ06_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21738.1"
                     /translation="MTLLELVPAPEELLLPEPLPELELAPELLVGAEPLELEVAPELE
                     VAPELEVAPELEWPPELELAPELLVGAEPLELEVAPELEVAPELEVAPEPEWPPELVL
                     LPLVPPPELPPCEPPVVPLELPDCFRFCTITVAEAGLPKLAWPSMLFREIEKSLPAAP
                     AVTGTRIVFALVSPAFQLRVPLLGV"
     regulatory      complement(4922..5014)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT          999 a         1576 c         1669 g          954 t
ORIGIN      
        1 atctggaaga caatcaggct gcggcacgcg gccgcttacc ggatgccgcg atgcgacagc
       61 ggatggagaa attctgggac gcgcgagcct aaaggcaatc cgctagcctc gccggcgcct
      121 gaacagagtt tccggcacgg atcgcacgat gaacatgatt gcacgccaga accacggcgt
      181 gtagatcatc actgaaccgc gatccatggc gcgtcgaata tcgtgtgcca cgcgagccgg
      241 tttggcccat agcgcacctt tgggaaaggc tgcggtcatc ggagtgtcca cgaatcccgg
      301 tttgattgtg agcacgcgta cgccgcgcgc gtacagctgc tggcccaatc cgctcaggaa
      361 agcggtcacc gaagctttgg cggcgccgta ggcgtagttg gaatggcggc cgcggtcgcc
      421 ggcgaccgat gaaatgaccg ctagtacacc tgcgccccgc gcagcaaata ctcgcgccaa
      481 cgccagacac agcaccatga cgctcagcgc gttgacggtg aattcctccc gcagtgcttc
      541 gatcgagtct tcgcaggcgt tttgatccga caacgtgccg tgtgcgatca gcgcctgatc
      601 cagcccgcct aggctctcaa cgcccgaggc aatcagggca ggcagccgtt ccagctctcg
      661 tacgtcaatg actaccgtgc ccgcgatgct tgcgccacgc agtctcaggt cgtcggcgat
      721 cgtctgcagc agcggcgcgt tgcggcccgc gagaaacagc gcatccccgc gcgcggcgaa
      781 ttcgcgtgcc gtcgcctccg cgatcgccga tgtcgcaccg acgatcagga tgcgctgcac
      841 gagggctgtc cggtcacgcg gcgccagaac gatgaggaaa agcgcggatc gacgaactgc
      901 cggaacgctt cccaggccgg gaagtacctt tggaacgatg cagccgacat gcgtgcatcc
      961 ttggccggat agaccgcgcc accagcctgt tcggtaacag agtcaaacga tgccagaagc
     1021 ctgagcgtgt ccgcccctcg gttgggaaaa tccatggcca cggtgacgcc cggtcgtgcg
     1081 aacgacaaca ggccgagcga aggggctgag ccgaatttct tcagcacgct gacgaaggaa
     1141 ccttgacggt ggcgttcgag cagaccgagc attgtcttga atgcgtcggt cgccccctgt
     1201 gcgggcagga cacattgata ctggaaaaaa ccccgcggac cgtatacccg attccaatcg
     1261 aggatgcgat ccagaggaaa tagaaagcgt tgctgcagcc attggcgctg ctttggccga
     1321 gttgccgagc gacggtagta gagtccgttc aggatccgtg tggtgaactg cgagaccact
     1381 gagaacggcg gagtgagcgg gacgcgcacc tgtccctgcc ggatcggagc tgggtcggtg
     1441 atggcgcaat gattcgcccg catgaacacg ccacgaccgc gttgctgcgc cggcgccgtg
     1501 ctgtcgaacc atgcgacggt gtactcatgc tcgtcatccg atgcggcgct gagcgccagg
     1561 aattcctcca tgttggcgaa gcgctgtgtt tcgccttcga tccacgggcc gtcgatcgtg
     1621 cgcagctgca ggcgcgcgct gcggatcagt ccggtcaggc caaggccacc gatggtggca
     1681 cggaacaatt ccgtattctg atgaggcgag cagatcagga actcgccgtt cgagcgcaag
     1741 agttcaaaat cgagcaggtg atggcagaag gtgccgaccc gatggtgatt cttgccgtgt
     1801 acatcgttgg ctatcgcgcc gccgacggtg accaacgcgg tgcctggcac caccggaagc
     1861 atccaatgac gtggagcgag cagctcaatg atttcgctca acagcacacc cgcctcgcac
     1921 tccagcactc cagtggctgc atcgaaggcg atcagatgat cgagcgagcg cgtactgagc
     1981 aacgtgccgc cgtcgttcag acagacatcc ccgtagctgc gaccgttacc gtagggcagc
     2041 aacggcgcga agtccggcag gggctcgaat cggcttctga gagctacgat ctgctgattg
     2101 tggcgaggcc aacggcccca cgagtctccc agtgccacgg ttgtgtctag attgccaggt
     2161 acacgatcac gaccagcaat cccagagtga ctaggctgac gccgtcctgt accgcgaata
     2221 ccagcgggtc gtcgtgcaac cggccgcgcg ccgcgagcat ccacgcacgg ctgatccaga
     2281 acagcatcag cgggcagatc aaccacaaca cgtggtgata gcggtaaatc gcttgcgatt
     2341 cggggctgtt gatgtagaga gcggtcacga cgatagcgca cagaccggag gccgagccca
     2401 ggctcatgat ggccggcatg tcttccgcgc tgtagtcgcg gccccggcct gccgggcgtt
     2461 cggtgcgccg tgtggattcc agttcggcgt aacgtttgat gaacgccagg ctcatgaaca
     2521 tgaacacaga gaatgcgagc agccagaacg atactggcac gttactggcg gcggcgccgg
     2581 cgatgatacg gatcgtatag agcgtcgcga gcaccatcac gtcgagcaaa gcaacgcgtt
     2641 tgaggcggac cgagtaggca caggtcagca ggtagtagcc aaccagcgcc aatgcgaatc
     2701 gcaggtccag agccgcggac accgcggcgg cgccgagcag cagcaacagg gacgcccagg
     2761 cgcccgccgc cggtgagatt tcaccgcagg ccaggggtcg ggtgcgcttg tgggggtgtg
     2821 cccggtcggc cgccagatcg aacagatcat tggtcaggta gacgctggac gcacagagtc
     2881 caaaggcaat gaatgccagg gcgcagctgc gcagtaccgt cgcctggaag atcgtgtgcg
     2941 ccagcagggc aggcagaaac accagagtgt ttttgaccca ctgatgcaag cgaacggcgc
     3001 gcatccagat gctgatggag ggggttgaac gggggaacac ctgctcaacg tccgacacgc
     3061 gcctgacctc gttggccagt gctgtatcgc cgaccaccac cgcgcgtctg gcggctttcc
     3121 agacgatctg atccgaccgc tgactgccgg cgtagtcaaa tccctgttcg ccgaaacgcg
     3181 ccaccagggc gctgcgcttg cgttcggcgg gcgatccgcc ccgcggatcc acccaggcgt
     3241 actcgtcaaa taaccccagc ggtgtcgtga cctgatccgc gatgcgccgg tcaccgttga
     3301 cgaccagtac cagccgtctt ccggagcgtc gctcattctg cagccaatga atcaggtcat
     3361 gtcgcagtgg cagacccaaa acatcgacat cagtggtgga gtcggggcgg tcagtaggtc
     3421 gtgcggccag cgctctgacc ggggactgtt tggcgagcag gagcaatagt tcgagctgca
     3481 gcggattcgc cgtcagtgtg ccatcgaagt caatgcagag cggaatagcc gcgtcggtca
     3541 tccggatcct accgtgcgct ggcggcagtg tgccatcagc gctcggcaat tactaacgga
     3601 tccctgtcga gcctgagctg gcgagcgctt catgcgctcg ccagctcttt gacactcatc
     3661 ccggcagcgt gccgctgagg gcgtggcggc gcgcgacagg tgggtgtctc aagttagcta
     3721 gagagtggcc tcgaccgcgc tggacgcgat gctttcggct cctgcactgt tgaccgaggt
     3781 gagcgtgaag taccagttgc ccgcagcaag ttcactgatc acgtaggtct gcacgcctac
     3841 cgttttgatg ctgatcttgt tggtcatggc attggtgctg ttgccatagt agatgttgta
     3901 gccgactaga ttggtcagcg cagtaccatt ggtgttttcg gtcggcgctg cccaggaaag
     3961 ggtgacactg ttggagctcg tgcccgcgcc cgaggagctg ctgctgcccg agccgctacc
     4021 cgagctggag ctcgcgcccg aactgctggt tggagctgaa ccgctcgagc tggaggtcgc
     4081 acctgagctg gaggtcgcgc ctgagctgga ggtcgcgcct gagctggaat ggccgcccga
     4141 gctggagctc gcgcccgaac tgctggttgg agctgaaccg ctcgaactgg aggtcgcgcc
     4201 tgagctggag gtcgcacctg agctggaggt cgcacctgag ccggaatggc cgcccgagct
     4261 cgtgctgcta ccgctggtgc cgccgccaga gctgccgccc tgcgagccgc ctgtggtgcc
     4321 actcgagctg cctgactgct tcaggttttg cacgataacc gtcgcggagg ccggattgcc
     4381 gaagctggcc tggccttcaa tgctattcag ggagatcgag aagtccttgc cggctgcgcc
     4441 ggccgtgacc ggcaccagaa tcgtcttcgc gctggtgtca ccggcgttcc agctcagggt
     4501 tccgctgctc ggcgtgtagt cctcgccaga tactgctgag ccgttgatcg tgccgtaggt
     4561 tacggtcgcg tctcccgaag ctgacccgct gcggtagacg gtgatgatgg cactggacgc
     4621 gggagcgacc tgataatcag cggctgacag tgcgacggtc cccaccgccg aagctgtcgc
     4681 gctcgaaggg gtgctaccgc cggtgccgga gttcgatgac tcggtcgctc cgccacaccc
     4741 ggtgagtgct gcgccgacca tcagaattcc gatcaggctt atccctcgtc cctttgccct
     4801 aacttgcatt tcgcgtcgcc ctcgtagttc aggttgttcc ccgagctctc cgggcggcga
     4861 tcgtcttgat ggtgggaagg aggttcgacg gctgcagcac gatcgtgacg atcgtgtgac
     4921 tgccggcaac cccgctgtct caccgatcga atgactggat cggagaccgg aggctttgcg
     4981 tccccgcctt gcggcgggtt tgcccttaac tcgcgagcgt agtaaacgaa aaaaaaaact
     5041 cgttaacaaa gtgtctgcag cgacacacac gtcgccatct tgcgacgctg gttttcacgg
     5101 catttgagtg atcccagaga ggaaatctcg gtcggattta ttgaagtgtt tcgttgtcaa
     5161 tcgcgcgatc gtgcttcgtt gtgtgacgaa gttgtctt
//
LOCUS       DATMRM010000432         2890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2109242, whole genome shotgun sequence.
ACCESSION   DATMRM010000432 DATMRM010000000
VERSION     DATMRM010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2109242"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VNZ06_13130"
     CDS             <1..528
                     /locus_tag="VNZ06_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWW21739.1"
                     /translation="AVTTSNYCGPSATEIINGQPVLSTNCPNQVNDYRFTSASGFSQP
                     SIVAPSGPEAPSGTQLPVAPKFKANLVARYTFPLANGDAHVQAGYVYQTSSEPLLRLV
                     DQINLGKLPSYGLLDLSSGLTRNRLSLELTLSNVLDKRAQLTRFVECATTTCTQPYVI
                     PTQPRTVMLRFGQKF"
     gene            complement(648..2057)
                     /locus_tag="VNZ06_13135"
     CDS             complement(648..2057)
                     /locus_tag="VNZ06_13135"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HWW21740.1"
                     /translation="MAAINGLIIIDPLRWHMALRTLAVGFAAVLTLVSPGYAQETTQA
                     VDPRIAAALKEVSPVRIQANIERLVSFGTRATLSAQDPASIAAGRGIGAAREWIKSQF
                     VRYSKECGGCLEVKTDAFTQVPAERIPQPTEITNVYALMKGTDPQAAKRIVLVTGHYD
                     SRVTDVLDFKSAAPGANDDGSGTAVSLECARVLSKLKFPSTIIFLTVAGEEQGLNGSR
                     HFAQMAKAQGWDIVAALNNDIVGGDKGVGQDPGVVRVFSEGVPVSAGDKELKQIRSLG
                     GESDSPSRELARYIAEIGAAYDPGVSALLVFRLDRYLRGGDHSAFNQQGYAAVRFTEY
                     REDFDHQHQTLRTENGIEYGDLAKFVDFEYVAKVARLNALTLAALASAPGSPSNVRLV
                     TSNLDNDSTLKWEPSGDASGYEVIWRATSSPVWEHAQNVGNVTTTTLKLSKDNVILAV
                     RAVDREGHRSLPVVPLPER"
     gene            complement(2160..>2890)
                     /locus_tag="VNZ06_13140"
     CDS             complement(2160..>2890)
                     /locus_tag="VNZ06_13140"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HWW21741.1"
                     /translation="SAAGTIGWCASMSAMGKMSMPGGWTMSMVWMRMPGQTWPAAGAS
                     FVGMWVVMMVAMMLPCLVPMLWRYRQALAHTGKRRLGPLTALVGVGYFFVWTVLGMIA
                     YPAGVALAAVEMQQAALANAVPIAAGVVVLIAGGLQFTAWKARQLACCRAAPGRGCLL
                     PADAGTAWRCGLRLGLHCSSCCATLTAILLVVGVMDLRVMAFVTAATTFERLAPAGER
                     VARATGALIVASGMWLIAHGAGLV"
BASE COUNT          546 a          985 c          859 g          500 t
ORIGIN      
        1 gccgtgacca cttcgaacta ctgcgggccg agcgcgaccg agatcatcaa cggacagccg
       61 gtcctgtcga ccaactgtcc taaccaagtc aacgactacc ggttcacctc tgcaagcggc
      121 ttctcgcaac cgagcatcgt cgcaccgtcc gggcccgagg cgccatctgg tacccagctg
      181 ccggtcgcgc cgaagttcaa ggccaatctg gtcgcgcgct acacgttccc gctcgccaat
      241 ggggacgccc atgtacaggc cggctacgtg tatcagacga gcagcgagcc gctcttgcgc
      301 ctggttgacc agatcaatct cggcaaactg ccctcctacg gcctccttga tctgtcgagc
      361 ggcctgacgc gcaaccgcct gtcgcttgag ctgacgctga gcaacgtgct cgacaagcgg
      421 gcccagctca cacgcttcgt cgagtgcgcg actacgacct gcacgcagcc gtacgtcatt
      481 ccaacgcagc cgcgaacagt catgctgagg ttcggacaaa agttctgagc atctggtgcg
      541 gcggggcctg cgccgagtgg accagtcagg agcctccggc tacatccgca gcggtgtggc
      601 cgggggtgcg cggccgcaag actgcgcgcc catggtccct tgcgctctca gcgctcgggc
      661 aacggcacga ccggcaggct ccgatgccct tcgcggtcga ccgcccgaac tgccaaaatc
      721 acgttgtctt tggagagctt aagcgtggtg gtcgtgacat tgccgacgtt ctgagcgtgc
      781 tcccaaacag gactgctcgt ggcccgccag atgacctcat acccactggc gtcgccggag
      841 ggctcccatt tgagggtgga atcattgtcg aggttactgg tcaccagtct gacgttgctc
      901 ggtgatcccg gcgccgaagc gagcgcggcc agcgtcagtg cattcagtcg cgccactttc
      961 gccacgtact cgaagtccac gaacttcgcc agatcaccat actcgatgcc gttttcggtg
     1021 cgtagcgtct gatgctggtg atcgaagtct tcgcggtact cggtgaagcg gaccgcggcg
     1081 tatccttgct ggttgaaggc ggaatgatcg ccgccgcgca aataccgatc aagccggaag
     1141 acgaggagcg cgcttacgcc cggatcatag gcggcgccaa tctctgcgat gtagcgcgcc
     1201 agttcccgcg acggcgaatc gctctcgccg ccgagagagc ggatctgctt gagttcctta
     1261 tcaccggcgc tgacaggcac cccctcggaa aacacgcgca ccacgcccgg atcctgtccc
     1321 acacccttgt caccaccgac gatatcgttg ttcagagcgg ccacgatatc ccatccctgc
     1381 gccttcgcca tttgcgcgaa gtgcctgctg ccgttcagac cctgctcttc gcctgcgaca
     1441 gtcagaaaga tgatggtcga gggaaatttc agcttgctca agactcgcgc acactcaaga
     1501 ctcaccgccg tcccgctgcc gtcatcattg gcacccggcg ccgcgctctt gaagtcgaga
     1561 acgtccgtga cgcgtgaatc gtagtggccc gtgaccagca caatgcgctt agcggcctgc
     1621 ggatctgttc ccttcatcag cgcatacacg ttggtgattt cggttggctg gggaatgcgc
     1681 tcggcaggca cctgtgtgaa ggcgtccgtc ttcacttcca ggcaaccgcc acattccttc
     1741 gaatagcgca cgaactgcga cttgatccat tcgcgcgcag ccccaatgcc acgcccggcg
     1801 gcgatggacg ccggatcctg cgccgagagc gtggcgcgcg tgccgaagct tacgagcctt
     1861 tcgatgttgg cctgaatgcg caccggcgag acttccttca gcgcagcggc gatcctaggg
     1921 tccactgcct gggtggtctc ctgtgcatat cccggggaca caagagtcag caccgctgcg
     1981 aagccgaccg caagcgtgcg cagggccatg tgccatcgca gggggtcgat aataattagg
     2041 ccgttgatcg ctgccatatc ggaatttatt ggggccaagg agggagtcga accctcgacg
     2101 cggcattatg agcgcggttc ttggctcaag ctaaccctgc acgccgaaga ctggattggt
     2161 cacacaagcc cggcaccgtg cgcaattagc cacatccctg atgcgacgat gagggctccg
     2221 gtggctcgcg cgacgcgctc accggcgggt gcgagacgtt cgaaggtggt agccgccgtc
     2281 acgaaagcca tcacccgcag gtccatgacg ccgacgacga ggaggatcgc cgtcagggtg
     2341 gcacagcaag agctgcagtg cagaccaagg cgcagaccgc atcgccaggc cgtgcccgca
     2401 tcagccggca acagacagcc gcgccccggt gcggcccgac agcacgcgag ctgacgtgcc
     2461 ttccacgcgg tgaactgcag cccgcctgcg atcaggacga ccacgccggc agcgatcggt
     2521 acggcattcg ccagcgccgc ctgctgcatc tctacggccg caagcgcgac gcccgccgga
     2581 taggcgatca ttcccaacac ggtccacacg aagaagtacc ccacacccac cagcgcggtc
     2641 agtggcccga ggcgcctctt gcctgtgtgg gcgagagcct gccggtatcg ccacagcatt
     2701 gggaccaggc acggcagcat catcgccacc atcatgacaa cccacatacc cacgaaggag
     2761 gcccccgcgg cgggccaggt ctgtccgggc atccgcatcc ataccatgga catcgtccac
     2821 ccgccgggca tcgacatctt gcccatcgcc gacattgacg cgcaccagcc gatcgtcccc
     2881 gccgcgctgg
//
LOCUS       DATMRM010000433         2537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_878293, whole genome shotgun sequence.
ACCESSION   DATMRM010000433 DATMRM010000000
VERSION     DATMRM010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878293"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VNZ06_13145"
     CDS             <1..278
                     /locus_tag="VNZ06_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21742.1"
                     /translation="TIMPVNWSSVWRHSLPSRFADHGALNLAVGIVGARQRPVPQGSL
                     EYRIQVKCNAVAERFNLPPAKIVVIAHMYQLHGRIGHPDPVRTGKAR"
     gene            complement(363..968)
                     /locus_tag="VNZ06_13150"
     CDS             complement(363..968)
                     /locus_tag="VNZ06_13150"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HWW21743.1"
                     /translation="MKALADEELVRTGCADGVRLGPAFLRLVGKLHTDVVAVAAPHLQ
                     SLSDDIGETVALGRISGRELAFIHVVVAEQELRVVPRVGANLPLATTAGGRALLALGA
                     DEEALMLLQLPDSKRTDSGELLKELKRVRRIGYAVDDDETTPGVVSLAVGVDTILGRF
                     AVSVPAPAVRVAAAGRPRIVERLLACRDVLLGEIGCNRPDE"
     gene            970..1425
                     /locus_tag="VNZ06_13155"
     CDS             970..1425
                     /locus_tag="VNZ06_13155"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWW21744.1"
                     /translation="MRWIVERGRSTAFAIGPTLSLPVFEGGRLRAQLKLREAQQQEAA
                     LAYQRTVPGAWNEIDNTLSALSTEQQRRTSLAANVAQSDHALQLSTSPYQAGAGTRLD
                     ALATQRTLLAAKLEWIRSDADVSLNAIRLYKALGGGWEGAAGETPLAAR"
     gene            complement(1646..1739)
                     /locus_tag="VNZ06_13160"
     tRNA            complement(1646..1739)
                     /locus_tag="VNZ06_13160"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1703..1705),aa:Ser,seq:gct)
     gene            complement(1948..>2537)
                     /locus_tag="VNZ06_13165"
     CDS             complement(1948..>2537)
                     /locus_tag="VNZ06_13165"
                     /inference="COORDINATES: protein motif:HMM:NF025210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWW21745.1"
                     /translation="YQVKTRVLSSLTDPLIPLDTEMKSGTLITFEEDETMEKAVISGI
                     AFQRDEARIAVMGVPDKPGIAYQILGPVADANIDVDMIIQNQSVEGKTAFTFTVGRGD
                     YQRAMDILTGQVKGHVQAEQVLGDPKVSKVSVVGVGMRSHVGIASTMFRTLSEEGINI
                     QMISTSEIKISVLIDEKYMELAVRALHKAFELDQA"
BASE COUNT          495 a          840 c          720 g          482 t
ORIGIN      
        1 tgacgatcat gccggtgaac tggtcgtccg tctggcggca ctccttgcct tcgcgcttcg
       61 ccgatcacgg cgcgctcaac cttgcggtcg gcatcgtcgg ggccagacaa cggcccgtgc
      121 cacagggctc acttgaatac aggatccagg ttaagtgcaa tgctgtcgcc gaacgattca
      181 acctgccacc ggccaagata gtcgttatag ctcacatgta ccagttgcac gggcgaattg
      241 gccaccctga cccagtaagg accggcaagg cccggtagtg tgtcgcgcca ggcttgtgct
      301 cccgcacctc gctggggtgc ggcagcaaag actgtgttca tatccgtcat ttccacctca
      361 tttcattcat cggggcgatt gcaccctatc tcacccagca agacatcccg gcacgccagc
      421 aacctctcca cgattcgtgg tcgtccggct gccgcgacac ggactgccgg agccggaacg
      481 gataccgcaa agcgcccgag gatggtatca acgccgacag caagggacac aacaccgggc
      541 gtcgtttcgt cgtcgtcgac cgcatagccg atgcgcctca cacgcttcag ttccttcagt
      601 agttcgccgc tgtccgtccg cttggagtct ggcagttgaa gcagcatcaa cgcctcctcg
      661 tccgcaccga gcgccagtaa cgccctcccg cccgccgtgg tcgcgagcgg caggttcgcc
      721 ccgacacgcg gcaccacacg cagttcctgc tcggctacca ccacgtgaat gaacgccagc
      781 tcgcggccgc tgatccggcc gagcgccacc gtttcaccaa tatcgtcgct cagcgactgc
      841 aggtgcgggg ccgccacggc taccacgtcg gtatgcagtt tgccgaccag ccgcaggaac
      901 gccggcccga gccgcacgcc atcagcgcac ccggtgcgca cgagctcctc gtcagccagc
      961 gccttcacga tgcgctggat cgtggaacgg ggtagatcaa ccgccttcgc gatcggcccg
     1021 acgctcagcc tgccggtctt cgaaggcgga cgactgcgtg cccaactgaa gctgcgtgag
     1081 gcacagcagc aggaggccgc gctcgcgtat cagcgtacgg tgcctggcgc ctggaacgag
     1141 atcgacaaca cgctctccgc gctctcgacg gagcagcagc gacgcacgag tctcgcggcg
     1201 aacgttgcgc aaagcgacca tgccttgcag ctctcgacgt cgccctacca ggccggcgcc
     1261 ggcacccggc tcgatgcgct cgcaacgcag cgaaccctgc tcgcggcaaa gctcgaatgg
     1321 atacgcagcg atgcggacgt atcgctgaac gcaatacgtc tgtacaaggc gcttggcggc
     1381 ggttgggaag gcgccgcggg cgaaaccccg ctcgccgcgc gctgacgaat cacgctgttg
     1441 cgtcacgtca ccgatgcaac agctcgcctt tccttgatcg accgcgggct atcgcacgat
     1501 tgctgctcca attttcccga ccagaaccgt gatgggtctg ccatgggaga cggacttacc
     1561 aggaaatccg tgatgggcta gctgatgggc tgaaaaagaa aaagccccgt aagtcgttga
     1621 acttacgggg ccttgtcacc gcttttggcg gagacggagg gattcgaacc ctcgatccag
     1681 gttttggccc agatgctccc ttagcagggg agtgccttcg acctctcggc cacgtctccc
     1741 aaactttcgg ttgcgcaggg agtcagcgca acgaagccaa gataatagcg ggccgaaccg
     1801 cgaaggtcaa tgtctgccgc acattttttg tgccagcttt gtcactgacg acgcaattaa
     1861 ttgacattaa cttgcgtcac attagccggg cgtaccgcac acattcagag atgaggtgct
     1921 cttgtacacc ccaccccgca cagcaaatta cgcttgatcc agttcgaacg ccttatgcag
     1981 cgcgcgcacg gcgagctcca tgtacttctc gtcgatcagc accgaaatct tgatttcgga
     2041 ggtcgagatc atctggatgt tgatgccctc ttccgacaac gtgcggaaca tcgtgctcgc
     2101 gatacccacg tgcgaacgca tgccgacgcc gaccaccgat accttcgaca ccttcggatc
     2161 gcccagcacc tgctcagcct gcacatggcc cttcacctgg ccggtgagga tgtccatggc
     2221 gcgctgataa tcgccgcggc cgaccgtgaa cgtaaaggcc gttttgcctt cgacgctctg
     2281 gttctggatg atcatgtcga cgtcgatatt cgcgtccgcc accgggccga gaatctgata
     2341 cgcgatgccc ggcttgtcgg gcacacccat cacggcgata cgagcttcgt cgcgctggaa
     2401 cgcgatgccc gagatgactg ctttttccat ggtctcgtct tcttcaaaag taatcagggt
     2461 gcccgacttc atttcagtgt cgagcgggat cagcggatcg gtcaggctcg acagcacacg
     2521 cgtcttcacc tgatatt
//
LOCUS       DATMRM010000434         3202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_879127, whole genome shotgun sequence.
ACCESSION   DATMRM010000434 DATMRM010000000
VERSION     DATMRM010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879127"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VNZ06_13170"
     CDS             complement(<1..260)
                     /locus_tag="VNZ06_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009315099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21746.1"
                     /translation="MLENVGCEPRHDWTLEQAESLFELPFADLIVTAQRVHRAWHEPN
                     SVQMSTLLSIKTGACPEDCAYCPQSVRFDTGLEAQPLMSLSQV"
     gene            complement(358..1338)
                     /gene="ubiA"
                     /locus_tag="VNZ06_13175"
     CDS             complement(358..1338)
                     /gene="ubiA"
                     /locus_tag="VNZ06_13175"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR01474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HWW21747.1"
                     /translation="MSRSLEAPPEIERVPAAVVAAEAAGAPPVLAPDSETRREHWLVR
                     RLADFGRLMRLDRPIGIWLLLWPELWALWIAAAGHPNLRLLSIFLCGTVLMRSAGCVI
                     NDLIDRNIDPHVKRTRSRPLAARVIGPYEALWLFGVLILSAGLLALQLNAFTLRLAFV
                     GVGLTVTYPLLKRFFPLPQLYLGLCFGWAVPMAFAATQGSVPRVGWLLLVAAVLWAGV
                     YDTMYAMTDRDDDLRIGVQSSAILFADLDRFMIGVMQLMMLFALMLVGRTLHFGPAYR
                     DGLISGAVFFLWQQWLIRHRQRDACLRAFHNNQYFGIAVFAGILLQYSAA"
     gene            complement(1436..>3202)
                     /gene="recG"
                     /locus_tag="VNZ06_13180"
     CDS             complement(1436..>3202)
                     /gene="recG"
                     /locus_tag="VNZ06_13180"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HWW21748.1"
                     /translation="TRVRCHGEVRRGPLGLEIVHPEYRRTETLNEPLPQTLTPIYPAT
                     EGMTQGRHRARVTRALTALTQHRVQDLLPEALLAHLGVPCLHAALEFVHRPPVGTELI
                     ELASGRHPAQRRLAFEELLAHQLSLLELRHQLRQDQAAALDDTSGLQARLLGSLPFCL
                     TPAQRRVLLEVERDLIGTTPMARLVQGDVGCGKTAIAALAAARAIGSGAQVALMAPTE
                     LLAEQHARTLCGWFDPLGVSVSLLTGSQPARTRRSALQAVASGRAQLCVGTHALFQDE
                     VRFSELSLAIIDEQHRFGVHQRLQLAAKSGTAVSGAPRMPHQLIMSATPIPRTLAMAL
                     YADLDVSIIDELPPGRTPVKTVVVPEEKRAAIVARIDQACRGGRQAYWVCPLIEESEE
                     LAASAAEETATALAEALPSLRVELLHGRMPAKQKEQIMRRFLAGAVHLLVATTVIEVG
                     VDVPNASLMVIENSERMGLAQLHQLRGRVGRGAHESSCVLLYRAPLSQMARARLAAIR
                     DSNDGFRIARRDLELRGPGEILGQRQTGLAQLRVADLMRDADLLDQARTVAEQMLQTS
                     PQSVAPLRARWIGEQEQYGRVG"
BASE COUNT          585 a         1056 c         1037 g          524 t
ORIGIN      
        1 acctgcgaca gcgacatcaa cggctgggcc tccaagccgg tgtcgaaacg cacgctctgt
       61 ggacagtagg cgcagtcctc ggggcaggcg ccggtcttga tcgacagcag cgtgctcatc
      121 tgtacgctgt tcggttcgtg ccaggcgcgg tgcacccgct gcgcggtgac aatcagatcc
      181 gcgaacggca gctcgaagag gctttccgcc tgctccagtg tccagtcatg acggggctcg
      241 caaccgacgt tttcaagcat gaggtctccc cctgaggttg agtcggctgg catgttcggc
      301 ggccgcccgc agcgctgtca acctgcagga tctcttgtgg ttgacgagcg cagggaatca
      361 ggcggcggag tattgcagca ggatgccggc aaacaccgcg atcccaaagt actggttgtt
      421 gtgaaatgcc cgcagacagg catctcgctg gcgatgtcgg atcagccact gctgccacag
      481 aaagaatacc gcgccgctga tcagaccgtc tcgataggcc gggccgaaat gcagggtgcg
      541 acccaccagc atcagcgcaa acagcatcat cagctgcatg acgccgatca tgaagcgatc
      601 cagatccgcg aacagaatcg cgctcgactg cacaccgatg cgcaggtcgt cgtcgcgatc
      661 ggtcatggca tacatcgtgt cgtagacgcc ggcccacaac acggcggcga ccagcagcaa
      721 ccatccaaca cggggcacag agccttgcgt tgcggcgaat gccatcggca ccgcccatcc
      781 aaagcaaagc cccagataga gctgcggcag tggaaagaac cgcttgagca gcggataggt
      841 caccgtcaat ccaacgccaa cgaaggccag tcgcagggtg aacgcgttga gctgcaacgc
      901 cagcaaccct gccgacagga tcaacacgcc gaacagccat agcgcttcgt aggggccgat
      961 cacgcgtgca gcgagcggtc gcgagcgcgt gcgcttgacg tgcggatcga tgttgcggtc
     1021 gatcagatcg ttgatgacgc agcccgcgct gcgcatcaga accgtgccgc acaggaagat
     1081 gctcaacagg cgcagattcg gatgccccgc ggcggcgatc cacagtgccc atagttccgg
     1141 ccacagcagc agccagatac cgatcgggcg gtccagacgc atcagacggc caaagtcggc
     1201 gagccggcgc accagccagt gctcgcgtcg tgtttcggag tcgggtgcca ggacgggagg
     1261 cgcaccggcc gcttcagccg caaccaccgc cgccggtacg cgttcgatct cgggaggtgc
     1321 ttccagcgag cggctcatgc gcggtccggg gcgcggcgca gcaagcgcgg ctccatcggg
     1381 gggcgtatcc ggcgatgcgt cttgcgccga gatgagcacg agctcaatct gggtatcacc
     1441 cgacgcggcc atattgttcc tgttctccaa tccagcgagc gcgcaacggc gcgacgctct
     1501 gcggcgaggt ttgtagcatt tgctcggcca cggtgcgcgc ttggtcgagc aggtcggcgt
     1561 cgcgcatcag gtccgcgacg cgcagctgcg ccaatccggt ctggcgttga ccgagtattt
     1621 ctccgggccc acgcaactca aggtcgcgcc gcgcgatacg aaagccgtcg ttgctgtcgc
     1681 ggatcgccgc caggcgtgca cgggccatct gtgacagggg tgcccgatac agcaacacgc
     1741 agctgctctc atgcgcgccg cggccgacgc gtccgcgcag ctggtgcagc tgtgcgagcc
     1801 ccatgcgttc agagttttcg atgaccatca agctcgcatt cggcacgtcc acgccgactt
     1861 caatcaccgt ggttgccacc agcaggtgca ctgcgcccgc caggaagcgc cgcatgatct
     1921 gttctttctg cttggctggc atgcgaccat gcagcagttc gacgcgcagc gagggtagcg
     1981 cttcggccaa cgccgtcgcg gtctcctcgg cggcgctcgc cgcgagctct tcggattcct
     2041 cgatcagcgg gcacacccaa tacgcctggc ggcccccgcg gcaggcctga tcgatacgcg
     2101 ccacgatcgc ggcgcgtttc tcttccggta caaccaccgt ctttacgggc gtacggcccg
     2161 gtggcagctc gtcgatgatc gagacatcca ggtctgcgta cagtgccatg gccagggttc
     2221 gcggtatcgg cgtcgcactc atgatcagct gatgtggcat gcgcggggcg ccggacaccg
     2281 ccgtgccgct cttggctgcc agctgcagcc gctggtgaac gccaaagcga tgttgttcat
     2341 cgatgatggc gagtgaaagc tcgctgaagc gcacctcatc ctgaaacagc gcgtgcgtac
     2401 ccacacacaa ttgagcgcgt ccgctcgcca cggcctgcaa tgcgctacgc cgggtgcggg
     2461 cgggttgcga acccgtcagc aggctcacgc tgacgcccag cggatcgaac cagccgcaca
     2521 gcgtgcgggc atgctgctcg gccagcagtt ctgttggcgc catcagagcg acctgcgcac
     2581 cgctaccgat cgcgcgagcc gcggccaagg cggcaatcgc ggtcttgccg caaccgacgt
     2641 cgccctgaac caggcgcgcc atgggggtgg tgccgatcag atcacgctcg acttcgagca
     2701 gcacccgccg ctgcgccggc gtcaggcaga acggcagtga gccgagcagt ctcgcctgca
     2761 gccccgaggt gtcatcgagc gccgctgcct gatcctggcg cagctgatgt cgcagctcga
     2821 gcagcgagag ctgatgcgcg agcaactctt cgaacgccag gcgacgctgt gccggatgtc
     2881 gaccactcgc aagttcgatg agctcagtgc ccaccggcgg acgatgcacg aactcgaggg
     2941 cggcgtgtaa gcagggcacg ccgaggtgcg ccaacagcgc ttccggcagc agatcctgca
     3001 cgcgatgctg cgttagtgcg gtgagcgcgc gggtaacgcg tgcccgatgc cgcccctgcg
     3061 tcatgccctc ggtcgccgga tagatcggtg tgagggtctg aggcagtggt tcattcaagg
     3121 tttcggtgcg ccggtattcc gggtgcacga tctccaatcc gagcgggccg cgtcgtacct
     3181 ccccatggca gcgcacgcgc gt
//
LOCUS       DATMRM010000435         4989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4616594, whole genome shotgun sequence.
ACCESSION   DATMRM010000435 DATMRM010000000
VERSION     DATMRM010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4616594"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VNZ06_13185"
     CDS             <1..849
                     /locus_tag="VNZ06_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HWW21749.1"
                     /translation="DAHAILGRDVRQWVLAATPAQLYANASAQLLCCLQALAAWRALD
                     LQQALPGEEIVLAGYSVGELASWGCAGLIAPADVLRLAIVRAEAMDRAAGSDTGMASV
                     RGLARASMERLCRAHGCEIAIALSEDSVVVGGRREALASLVREALASGARRSTALPIT
                     VAAHTSLLAAASTELRARLCEVPLAGGLLAGVRLLSGIDGDTVLDRAAGLDRLAQQLS
                     HTLDWHACLTACREAGVTRVLELGPGKALANMAREALPQARCRALEEFRSLDGVHTWL
                     RADRQE"
     gene            822..1238
                     /locus_tag="VNZ06_13190"
     CDS             822..1238
                     /locus_tag="VNZ06_13190"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HWW21750.1"
                     /translation="MAARRPTRIVPAAPGGALKRRYEALCALALKLPGVEISSSYRTP
                     SIKVKGKFLGRLRTEAEGAFAIRCDFLDRQILMQAHPAAFFVTDHYLNYPMVLVRLDQ
                     VGRRELSDLLERAWRMVATPALIRQKEEGSASSPRQ"
     gene            complement(1224..4235)
                     /locus_tag="VNZ06_13195"
     CDS             complement(1224..4235)
                     /locus_tag="VNZ06_13195"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWW21751.1"
                     /translation="MIPSRQGVIRAQDREDGPLMDKGPTSIAEARPLWSGWLVLIVAG
                     SIALLVFIAWQASRSLETLALRADWLNHTERVRYEIGRVLQSLTDVETDERGYAISGN
                     DSFLQPFQDAGSGLASELASLRQLTSDNPRQQALIQTLSRIVQQRIQQAQAVIEKARA
                     GDLAGARAEVAQGTGTSSTNEARQIIAQMQDEEKRLLGIRAEADARARSLATSLFLAL
                     GGLAILLLALIALVTARDAARLHRTERELAATLRSVGDAIISTDSQGIVRFVNPVAEK
                     LTGWLQPDARGAPLEQVFHIVNERTRARVESPVTRVLREGTIVGLANHTLLIARDGSE
                     RPVEDSGAPVFGANGQLAGVVLVFRDATLERAAQRAIQVSEQRFRAAIAAIEGVLWTN
                     TAEGEMRGEQPGWAALTGQSAAEYQGLGWSKAVHPQDAQPTINAWLEAVHSKQPFRFE
                     HRVRDRSGDWRAFSIHAIPVLEPNGAIREWVGVHADITEQRQFESQLRERERRFAALA
                     NSIPQLAWTNRADGTHEFFNRQWYEYTGLTPTESVRPEAWEKVVHPEDYPHSHRLWMQ
                     VLGTGERYDAEFRMRRHDGVYRWFLVRANADRDEQGHIIEWFGTCTDVDASKRTEEEL
                     RRTEASLREADVRKDVFLATLSHELRNPLAPIRTAARLLEMPTLSPQDLERSRAIITR
                     QVRHMASLLEDLLDVSRITRGALTLKKQCVHVQQRVAEAIETVQPALEARKQTLDLQL
                     PPDPIEIAADPVRLVQIVANLLANAAKYTPAGGYIALRALIQEDQLVLSVRDTGIGLA
                     PENLSKVFEMFTRISAITDRTEGGLGIGLALVKGLVELHGGRVEAHSEGVGRGSEFRV
                     MLPGPVPASARRASQEPSLPKGDGRAAGLRVLVADDNRDAAETLGMLLSVAGYQVELC
                     HSGPEAVSKITQWRPNVAILDIGMPGMDGFEVARQVRAQSWGNQIRLIALTGWGQEED
                     LRASRSAGFDHHLTKPVDLPELEQVIASLSG"
     gene            complement(4245..>4989)
                     /locus_tag="VNZ06_13200"
     CDS             complement(4245..>4989)
                     /locus_tag="VNZ06_13200"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWW21752.1"
                     /translation="HSKPDAPLPAYATPLSIADPDGEQLAALILAADVAHDSGLWVLH
                     LHGNADSAFSAGQLRHAGQLHALGLNVMVFDYRGFGHSAGTASESHIEEDAEAAYQNL
                     IRRGVDPSRIILWGHSLGSGPAVWLASRYPAAALVLFGAYASIPEVAQDTYPWLPVRW
                     LVGIHFDSLDRIGSVRAPVLIAHSRQDALVAFHHAQELFAAAHEPRQLLELPGPYTDG
                     LGGHVDSLYDHLDLIVPWLQKLTGASLTP"
BASE COUNT          824 a         1631 c         1652 g          882 t
ORIGIN      
        1 gacgcacacg ccattctcgg ccgggatgtc aggcaatggg tcctggccgc gacaccggcg
       61 cagctgtacg ccaatgcatc cgcgcagctg ttgtgctgcc tgcaggcgct cgcggcgtgg
      121 cgtgcactgg atctgcagca ggcgctgccc ggagaagaga tcgtgctcgc gggttacagc
      181 gtcggcgagc tggcatcgtg gggatgcgcc ggattgatcg cgcccgccga cgtgctgcgt
      241 ctggcgatcg tgcgtgccga ggcgatggat cgtgccgcgg gatcggacac cgggatggcc
      301 tcggtgcgcg gcctggcgcg tgcgtcgatg gagcgtttgt gtcgcgcaca cggttgcgaa
      361 atcgcgattg cgctgagcga ggacagcgtg gtcgtgggcg gtcggcgcga ggcgctggca
      421 tccctggtac gcgaggcgct ggcttcaggt gcgcggcgca gtaccgcgct gccgatcact
      481 gttgccgccc atacctcact gctggccgcg gcgagcaccg agttgcgcgc gcggctgtgt
      541 gaggtaccct tggccggcgg cctgcttgcg ggggtacggc tgctgagtgg catcgatggc
      601 gataccgtgc tggacagggc ggcaggactc gaccgactcg cgcaacagct ctcgcacacg
      661 cttgactggc atgcctgcct gaccgcctgt cgcgaagcag gggtcacacg tgtgctggaa
      721 cttggacccg ggaaagcctt ggcgaacatg gcgcgcgagg ccttgccgca ggcgcgctgt
      781 cgcgcgctcg aggaattcag gagtctggac ggggtgcata catggctgcg cgcagaccga
      841 caagaatagt tcccgccgca cccggcggcg cattgaaacg tcgctatgag gcgctgtgcg
      901 cgctggcact gaagctgccg ggagtggaga tttcaagctc ctaccgcaca ccctcgatca
      961 aggtgaaggg caagtttctc ggacggctgc gcaccgaggc ggagggtgcg ttcgcgatcc
     1021 ggtgtgattt tctggatcgc cagattctga tgcaggcgca tccggcagcg ttcttcgtca
     1081 ccgatcacta tctcaactat cccatggtgc tggtgcgcct cgatcaggtc gggcgacgcg
     1141 aactcagtga tctgctcgag cgagcgtggc gcatggtggc cacgccggca ttgatccggc
     1201 agaaggagga gggcagcgcc tcttcacccc gacagtgaag cgatcacttg ctccagttcc
     1261 ggcagatcga ccggtttggt caggtgatga tcaaaccccg ctgagcgcga tgccctcaga
     1321 tcttcttcct gaccccagcc ggtgagggcg atcagccgga tctgatttcc ccagctctgg
     1381 gcacgcacct gacgcgcgac ctcgaaacca tccatgcccg gcatcccgat gtcgaggatc
     1441 gcaacgttcg gccgccactg cgtgatcttg ctgacggctt ccgggccgct gtggcacaac
     1501 tcgacctgat agcccgcgac cgacagcagc atccccaggg tttcggccgc atcgcgattg
     1561 tcgtcggcga ccagcacccg cagtcctgcc gcccgcccat cgccctttgg cagcgatggc
     1621 tcctggctcg cgcggcgcgc ggaggccggc acgggtccgg gcaacatcac ccggaattcg
     1681 ctgccgcgac cgaccccttc gctgtgcgcc tcgactcttc caccgtgcag ttcgaccaga
     1741 cctttgacca gtgccagtcc gatacccagt ccaccctcgg tgcgatccgt gatcgctgag
     1801 atgcgggtga acatctcgaa cactttggac aggttctccg gcgccagacc gatgcccgtg
     1861 tcgcgcaccg acagcaccag ctgatcctcc tgaatcagcg cgcgcagcgc gatatagcct
     1921 ccagcgggcg tgtacttggc ggcattggcc agcaggttgg cgacgatctg aaccagcctc
     1981 accgggtcgg ccgcgatctc gatcggatcg ggcggcaact gcagatcgag cgtctgcttc
     2041 ctggcctcga gtgccggctg cacggtctcg atcgcttcgg ccacgcgctg ttgcacatgg
     2101 acgcactgtt ttttcaacgt cagtgcgccg cgcgtgatgc gcgagacgtc cagcaggtcc
     2161 tccagcagtg aggccatgtg ccgcacctgt cgcgtgatga tcgcgcggct gcgctcgagg
     2221 tcctgcgggc tcagtgtcgg catttccagc aaccgggcag cggtgcggat cggtgccagc
     2281 ggattgcgca gctcatgcga cagcgtcgcg aggaagacgt ccttgcgcac atcggcttcg
     2341 cgcagtgagg cttctgtgcg gcgcagctcc tcttcggtgc gcttgctggc atcgacatcg
     2401 gtgcaggtac cgaaccactc gatgatatgg ccctgctcgt cgcgatcggc gttggcacgc
     2461 accaggaacc agcggtagac cccatcatgg cgccgcatcc ggaactcggc gtcgtagcgc
     2521 tcaccggtac cgagcacctg catccacagc cggtgcgaat gcggatagtc ctccgggtgg
     2581 acgacttttt cccaggcctc aggtcgcacc gactcggtgg gtgtcaggcc cgtgtactcg
     2641 taccactggc gattgaagaa ctcgtgagtg ccgtccgcgc gattggtcca tgcgagctgc
     2701 ggaatgctgt tggccagcgc cgcgaaccgc cgctcgcgct cgcgtagctg gctttcgaac
     2761 tgccgctgtt cggtgatgtc ggcgtggaca ccgacccatt cacgaatcgc gccgttgggc
     2821 tcgagtaccg ggatggcgtg gatcgagaac gctcgccaat cgccggatcg atcacgtacg
     2881 cgatgctcga agcggaaggg ctgtttgctg tgcacggctt ccagccaggc gttgatcgtg
     2941 ggctgcgcgt cctgcgggtg caccgccttc gaccagccga ggccctgata ctccgccgcc
     3001 gactggccgg tgagcgctgc ccagcccggt tgctcgccgc gcatctcgcc ttcggcggtg
     3061 ttggtccaca gcacgccctc gatggcggca atcgcggccc gaaagcgctg ttcgctgacc
     3121 tggatcgcgc gctgcgcggc acgttccagc gtcgcgtcgc gaaagaccag caccacgccg
     3181 gcgagctgtc cgttcgcgcc gaacaccggg gcgcccgaat cctcgaccgg ccgttcactg
     3241 ccgtcccggg cgatcagcag cgtgtgattg gccagaccga cgatcgtacc ttcgcgcaac
     3301 accctggtca ccgggctctc caccctcgca cgcgtgcgct cgttgacgat gtgaaacacc
     3361 tgttcgagcg gtgcgccgcg agcgtcgggc tggagccagc cggtgagctt ctcggccacc
     3421 ggattgacga aacgcacgat gccctgcgag tcggtcgaga tgatcgcatc cccgacactg
     3481 cgcagcgtgg cggcgagttc tcgctcagtg cgatgcaggc gcgccgcgtc acgtgcagtg
     3541 accaacgcga tcagagcgag cagcagaatg gcgagcccgc ccagtgccag aaacaaggag
     3601 gtcgcgagcg agcgtgcccg ggcatcggct tcagcgcgaa tacccagcag gcgtttctcc
     3661 tcatcctgca tctgagcgat gatctgacgg gcctcgttcg tcgagcttgt gccggtgccc
     3721 tgggcgacct cggcacgcgc ccccgcgaga tccccggcgc gcgccttctc gatcacggcc
     3781 tgcgcctgct ggatgcgctg ctgcacgatt cgcgagagcg tctggatcag ggcctgttgg
     3841 cgcggattgt cggatgtcag ctggcgcagg ctcgccagct cgctggcaag gccggaaccg
     3901 gcatcctgga aaggttgtag aaaggaatca ttcccgctga tcgcgtagcc gcgctcatcg
     3961 gtttcgacat cggtgagtga ctgcaggacg cggccgatct cgtaacgcac gcgctcggtg
     4021 tgattgagcc agtcggcccg caacgccagc gtctcgagac tgcgcgaggc ctgccacgcg
     4081 atgaagacca gcagcgcgat gctgccggcc acaatcagca ccagccagcc tgaccacaga
     4141 ggccgcgctt ctgcgattga ggtagggccc ttgtccataa gcggtccgtc ctcacgatcc
     4201 tgcgcgcgaa tgaccccctg tctgctggga atcatatccg taagttacgg tgtcagcgaa
     4261 gcacccgtca gtttctgcag ccaaggcacg atgagatcca gatgatcgta gagcgagtcg
     4321 acgtgtccgc cgagaccgtc ggtgtagggt cccggcagtt cgagcagctg ccggggctcg
     4381 tgagcggccg cgaacagctc ctgcgcatgg tggaatgcaa ccagcgcgtc ctgccggctg
     4441 tgtgcgatca gcacgggtgc gcggaccgat ccgatgcgat cgagcgaatc gaagtgaatg
     4501 ccgaccagcc atctcaccgg cagccatgga tacgtgtcct gcgccacctc cggaatcgag
     4561 gcgtacgccc cgaacagcac cagcgcggcg gccggatacc ggctcgccag ccatactgca
     4621 gggccggaac cgagcgagtg cccccaaagg atgattctgg agggatcgac gccgcgtcgg
     4681 atcaagttct gatacgccgc ctcggcgtcc tcctcgatgt gagactcgga agcggttccc
     4741 gcgctgtgac cgaaaccgcg gtagtcgaac accatcacgt tcagtcccag ggcgtgcagc
     4801 tgaccggcat gcctgagttg accagccgag aacgctgaat cggcgttgcc gtgcaggtgc
     4861 aggacccaga gccccgaatc gtgtgccaca tcggccgcca ggatcagggc cgcgagctgc
     4921 tcgccatcag gatcggcaat gctcaggggc gttgcatagg cgggaagcgg tgcatcgggt
     4981 ttgctgtga
//
LOCUS       DATMRM010000436         5884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2735354, whole genome shotgun sequence.
ACCESSION   DATMRM010000436 DATMRM010000000
VERSION     DATMRM010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2735354"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VNZ06_13205"
     CDS             <1..1179
                     /locus_tag="VNZ06_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWW21753.1"
                     /translation="GLRLAGGLLRRSMAVGEHISDVHRQSMSSTADAFYALKQLKSHA
                     QERLLRNGFSARQQQLQASMLRYVRIAGLSDAGFQMFAACSLAATLWLAIHEFHFDIA
                     RTAILILIFARLVPGIATIPQTGREFLVAVPALKHVLNTIAQLEAFKAVPTPGTHAFR
                     TPWRVIRFEDVGFQYAEGGASILKHLNFTLRANTTTVLLGQSGAGKTTLVDLLIGLLR
                     PSEGRILIDDDPLTDVDPTGWRNRLAYVAQDGILFNSSIRDNLTFGLSAVSDDQLTRA
                     LALAGATEIVATAEHGLDTAVGDRGARFSGGERQRLALAQALLRQPALLILDEPTSSL
                     DAANRDLIGQTLDRLHGEMTIVIATHDAQLAMLADQTLHIRGGSGRAELRNLVSALHA
                     "
     gene            1194..2030
                     /locus_tag="VNZ06_13210"
     CDS             1194..2030
                     /locus_tag="VNZ06_13210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWW21754.1"
                     /translation="MNPQNLDGRRPEPMLADTQAAFDSVADIYDGPRGNNALIQRMRE
                     LIWQVIAQRVPRGGRLLDIGCGTGIDALHFATLGYSVLATDWSPQMVARAAARATTHG
                     LAERLQSARIGAQQLHQVSGQFDGAYSNFGPLNCVPDLREVARECARVLRPEGHMVFS
                     VIGRICPWELAYYLTRLRPGRALVRLARGMTPVRLNQHIVWTRYYTPREFYRQFAEQF
                     ELVHYRALSLFLPPPYLLGVYERHAALSRGLAWLDDRIGGWPALRAAGDHFLMILRKR
                     SS"
     gene            2047..3195
                     /locus_tag="VNZ06_13215"
     CDS             2047..3195
                     /locus_tag="VNZ06_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21755.1"
                     /translation="MDQAIVLRNGLVASAAGGTAEIRDLLLTPGDGASRRYEMDLQDH
                     TVLPALINAHDHLHRNAVPPLKSGPFANSYAWAAAFRSHFDDPEVKTALAVPAILRHW
                     QGGLKNALCGATTVMHHDSPSPILEHPHFPVRTLRPYGWAHSLHLEFGPPVAQSFQAT
                     PPNVAWFIHLAEGVDDVAAAELQELVALECLRENTVLIHGVGLSDADIGRVRAAGASV
                     VWCPSSNLAILGQTITPNRLRTLFSAGCLALGTDSRLSGAFDLLEELRVAARHSDFSP
                     CELLRLVTVDAGRLLRTASVHNDVIIFRSHSGDPFADALRLQRHELRAVVRDGELLVA
                     DLDFEEWFVRQRIAYTPIQLDGHPKLCASTLLSPHAGPMIDLEPGLVC"
     gene            3195..3866
                     /locus_tag="VNZ06_13220"
     CDS             3195..3866
                     /locus_tag="VNZ06_13220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWW21756.1"
                     /translation="MTTRELSPEEGYALWASNYPPCAHNAVMRAEERAMLGWLPASLH
                     GRRVIDAGCGSGRYSLHARQRGATVFGVDASLEMLQVAAGHDLRVAVGSLTHLPIASG
                     WADLVLCGLTLGHIPALPQALMELGRVLRDAGTALCSDFHPVGHALGWQRSFTLECQR
                     YAVRYVAHQYSDWHRACRMAGLTIEEVAEPRLDLADIPQDAHFDPRALELPVAIAFKL
                     RKGRG"
     gene            4026..5162
                     /locus_tag="VNZ06_13225"
     CDS             4026..5162
                     /locus_tag="VNZ06_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21757.1"
                     /translation="MNFRARLHSSAKLALLCAVSFLALSHAQRALAEPYLAIQNGYKC
                     VQCHVNPTGGGLRSTFGDVFAENVMAMNNLPAGAPVWLGQAVQDIIRVGGNLRADYSV
                     VTTPGAPSITEFDLQQFRLYADVTLIPNLLGIYVDEQVTPGAQTMEAYARIGSTSDWY
                     IKAGRFYLPFGWRLQDQTAFVRTASLISMALPDNGVEFGIEHGNLSAQIDLTNGVVNN
                     GTGYQVTSNVVWVESAWRVGASGSFTEARSANQFGNPPGNRWQEGLYAGVRTGPVAWL
                     TEIDLIRQAGNAAPGPGTQTQIPAFVEANWLIHKGNNLKLTFEYYDPEKQVSNNGQTR
                     WSVVYELTPIPFFQVRAGFRRNEGIPQAPTQNQTLAFVEAHAFL"
     gene            5213..>5884
                     /locus_tag="VNZ06_13230"
     CDS             5213..>5884
                     /locus_tag="VNZ06_13230"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HWW21758.1"
                     /translation="MLSILVAHSYFLRFDRKQQERAKPYPPLATLHVASMLRQAGHTV
                     RVFDAMLAEGLDEYEQLLADMRPQLVLLYEDSFNFLSKMCLERMRQASCQMISKARQD
                     GARVIAVGSDASDTPEPYLRAGADTVLFGEGLQSLQALSERLDTNPSLPISSLIEGLG
                     DLAAVLGGQLKVFKNAHPSPSRRQMDPPAWDLIDIERYRTVWQRAHGFFSLNMAASRG
                     CSFRCN"
BASE COUNT         1067 a         1828 c         1843 g         1146 t
ORIGIN      
        1 ggcttgcgtc tggccggcgg attgctgcgg cgctcgatgg cggtcgggga acatatcagc
       61 gacgtgcacc gacagtcgat gtcgagcacg gcggacgcgt tctacgcgct caagcagctc
      121 aagagccatg cgcaggaacg actgcttcgc aatggcttct ctgcccgcca gcaacagctg
      181 caggcgtcga tgttgcgcta tgtgcgcatc gcagggctct cggacgccgg tttccagatg
      241 tttgcggcct gcagtctagc ggcaacgctg tggctggcga ttcacgagtt tcacttcgac
      301 atcgcgcgca ctgccattct cattctgatc ttcgcgcgcc tggtgcctgg catcgccacc
      361 attccgcaga ctgggcgcga gtttctggtc gccgtgccgg cgttgaaaca cgtgctgaac
      421 accattgcac agctcgaggc attcaaagcg gtgcccaccc ctggcacgca tgcatttcgg
      481 accccgtggc gcgtcatccg tttcgaggac gtcggctttc agtatgcgga gggcggagcg
      541 agcatcctga agcatctgaa tttcaccctc cgcgcgaaca cgaccacggt gttgctcgga
      601 cagtccggtg ccggaaagac cacgctcgtc gatctgctga tcggtctgct gcgtccgagc
      661 gagggccgga tcctgatcga tgatgatccc ctgacggacg ttgacccgac gggctggcgc
      721 aaccgactcg cctacgtggc acaggacggc attctgttca acagcagcat ccgcgacaat
      781 ctcacattcg gcctgagcgc tgtcagcgat gatcagttga cgcgggcact ggcgttggcg
      841 ggcgcaactg aaattgttgc gacagcagag cacgggctgg acacagcggt tggagatcgc
      901 ggcgcgcgct tctcgggcgg tgagcgtcag cgcctggcgc tcgcccaggc gttgctgcgg
      961 cagcccgcgc tgttgattct ggacgaaccc accagctcgc tcgacgccgc caaccgagat
     1021 ctcatcggcc agaccctgga ccggctgcac ggtgaaatga cgatcgtcat cgccacacac
     1081 gatgcacaat tggccatgct cgccgatcag acgctgcata tcagaggggg tagcggccgg
     1141 gccgaactac gcaacctcgt gtcggcacta cacgcgtgac gatgcaggta gacatgaatc
     1201 cgcaaaacct cgatgggcgc cgccccgagc cgatgctggc cgatacccaa gccgcattcg
     1261 acagcgtggc cgatatctac gacggtccgc gcggcaacaa cgcgctgatc cagcgcatgc
     1321 gtgaactgat ctggcaggtg attgcgcagc gagttccgcg cggcggccgc ctgctcgaca
     1381 tcggctgcgg caccggcatc gatgccctgc actttgcgac gctcggctac tcggtgctcg
     1441 ccacggattg gtcgccacag atggtggcgc gagcggcagc tcgtgcgacc acgcatggct
     1501 tggccgaacg tctgcaatcc gcgcgcatcg gcgcccagca gctgcatcag gtcagcggtc
     1561 aattcgatgg tgcctactcc aatttcgggc cgctcaactg cgtgcccgat ctgcgtgagg
     1621 tcgcgcgcga atgcgcgcgc gtgctgcgac cggaaggcca tatggtattt tccgtgatcg
     1681 gccggatctg cccatgggag ctggcgtact acctgacgcg actgcgaccg gggcgggcac
     1741 tggtgcgcct ggcccgcggc atgacaccgg tcagactcaa tcaacacatc gtctggactc
     1801 gctactacac gccgcgcgaa ttttaccggc agttcgccga gcaatttgag ctggttcact
     1861 atcgggcgct tagtctgttt cttccgccac cctatctgct cggtgtctat gagcggcacg
     1921 cggccctgag tcgcggcctc gcgtggctgg atgaccgcat cggtggatgg cccgcgttgc
     1981 gcgctgcggg cgatcacttc ctgatgatcc tgcgcaagcg ttcaagctga ttgacggggc
     2041 cgggctgtgg atcaggcgat tgtgctgcgc aacgggctgg tcgcctccgc cgcgggtggc
     2101 acagccgaaa tccgcgacct gctgctgact cctggcgatg gcgccagcag gcgctacgag
     2161 atggatctgc aagaccatac tgtgctgcct gcgctgatca acgcgcacga tcacctgcat
     2221 cgcaatgccg tccccccgct gaagtcgggg ccgttcgcca acagctacgc gtgggcagcg
     2281 gcgtttcggt cgcactttga tgaccccgag gtcaagaccg ctctcgccgt acccgcgatc
     2341 ttgcggcact ggcaaggcgg tctgaagaat gcgctgtgtg gcgcgaccac cgtcatgcat
     2401 cacgattcac cgtcgccgat actcgaacat ccacactttc cggtacgcac gctgcggccc
     2461 tatggctggg ctcactcact gcatctggag ttcgggcccc ccgtggcgca gagctttcaa
     2521 gcgaccccgc ccaatgtcgc ctggttcatt cacctcgccg aaggtgtgga cgatgtcgcg
     2581 gcagccgagc tgcaggaact ggtcgcgctc gagtgcttgc gcgagaatac cgttctgatc
     2641 catggcgttg ggctcagcga cgctgatatc gggcgcgtga gggcggccgg agcgagcgtg
     2701 gtgtggtgtc cttccagcaa cctggccatt ctgggacaaa ccatcacccc gaatcgcctg
     2761 cgcacgctgt tcagcgcggg atgcctcgcg ctcggcaccg actcgagact cagtggggca
     2821 tttgacctgc tcgaggaact gcgtgtcgcc gcccgccaca gcgatttttc gccgtgcgag
     2881 ctgttgcgac ttgtgacggt ggatgcaggc cgtttgctgc gcaccgcttc cgtgcacaat
     2941 gacgtcatca tttttcgcag ccacagcggc gatccattcg ccgacgcact gaggttacag
     3001 cgccatgagc tgcgcgcggt ggtacgtgat ggtgagttgc tggtcgccga cttggatttt
     3061 gaggagtggt ttgtccgcca gcgtatcgcc tatacaccga tccaactgga cgggcacccc
     3121 aagctgtgcg ccagcacgct attgtcaccg cacgcaggtc cgatgataga tttggaacca
     3181 gggttagtct gctgatgacg acgcgagagc tcagccccga ggaaggttac gctctgtggg
     3241 caagcaacta tccgccttgc gcgcacaacg cggtgatgcg cgccgaggag cgagcgatgc
     3301 tcgggtggct gcccgcatcg ttgcacggcc gccgcgtgat agatgcgggc tgcggcagtg
     3361 gacgttattc gctgcatgcg cgccaacgcg gcgcgactgt gtttggggtc gacgcatcgc
     3421 tcgagatgct gcaagtggcc gcagggcatg atctgcgggt ggcggttggc agcctgactc
     3481 atctgccgat cgccagcggc tgggccgacc tggtgttgtg cggactgacg ctcggccata
     3541 ttccggcgct accgcaagcg ctgatggagc ttggacgcgt actgcgcgac gcgggcacag
     3601 ccctgtgcag cgattttcat ccggtcggac atgcattggg atggcagcgc agcttcacgc
     3661 tcgagtgcca gcgttacgcc gtgcgctatg tcgcgcatca atactcggac tggcaccggg
     3721 cctgccgtat ggcggggctg accatcgagg aggttgccga gccgcgactt gacctggcgg
     3781 acatccccca ggacgcccac tttgatccga gagcgctcga attgccggtc gccattgcct
     3841 tcaaattgcg caagggccgg ggttagatta tgttgagtct aaaaccaagc gggcgccgta
     3901 cggcacatat aggcggcggc actcaactat gttcgcccga aaacggcgta tgtccaggaa
     3961 ggggatccat gcttgcctct tgcgatcgaa tcgtcactcg ctaccgtcac caacacgaat
     4021 acccaatgaa tttccgcgcc cgtctacaca gttccgccaa gctcgccctg ctctgcgccg
     4081 tttcgttcct ggcattgagc cacgcgcaac gcgcactcgc agagccctat cttgccatcc
     4141 agaacggcta caaatgcgtc cagtgccatg tgaatcccac aggcgggggt ctgcgcagca
     4201 ccttcggcga cgtgttcgct gagaacgtca tggcgatgaa caatttgccg gcgggagctc
     4261 ccgtgtggct ggggcaggcg gtacaggaca tcatccgcgt tggtggcaat ctgcgtgctg
     4321 actatagtgt cgtcacgacc ccaggggcgc ctagcattac ggagttcgac ctccaacagt
     4381 ttcgactgta cgccgacgtc actttgatcc cgaatctctt agggatctac gtcgacgaac
     4441 aggtgacgcc cggggctcaa accatggagg cctatgcgcg gatcggcagc acctcggact
     4501 ggtacatcaa ggccgggcgc ttctatctgc cgttcggctg gcgtttgcag gatcaaaccg
     4561 cattcgtgcg taccgccagc ctgatcagca tggccttacc ggacaacggg gtcgaattcg
     4621 gaatcgaaca cgggaatcta tcggcacaaa tcgacttgac caatggcgtc gtcaacaacg
     4681 gtaccggcta ccaagtaacc tccaacgtcg tatgggtcga gtcagcctgg cgcgtcggcg
     4741 cgtcgggttc ctttaccgag gcccggtcag cgaatcagtt cgggaatcca ccggggaatc
     4801 gatggcagga gggtttgtac gctggcgtgc ggactggacc tgtggcgtgg ctgactgaaa
     4861 tcgatctcat ccgacaggcg ggcaatgcag caccgggacc aggcacgcaa actcagatcc
     4921 ctgccttcgt cgaagctaac tggctgattc acaagggcaa caatctgaag ctgacctttg
     4981 agtactacga tccggagaag caggtttcga acaatggcca gacgcgctgg agtgtggttt
     5041 atgagctgac tcccattccg ttctttcaag tgcgagccgg cttccggcgc aatgagggga
     5101 tcccgcaggc gcccacgcag aatcagacct tggcatttgt cgaggcccat gcgttcctgt
     5161 agcaaccgcg tgtagcgcca cgacagcgag tcgttcacaa ggccaccgat ttatgttgtc
     5221 catactggtc gcccattcct acttcttgcg cttcgatcgc aagcagcagg agcgcgccaa
     5281 gccctatccg ccgctagcaa cgctgcacgt cgcgtcgatg ttgcgccagg caggacacac
     5341 ggtgcgggtc ttcgacgcga tgctcgccga ggggttggat gaatacgaac agctgctggc
     5401 agacatgcgg ccacaactgg tgctgctgta cgaggacagc ttcaatttct tgagcaagat
     5461 gtgcctggag cgcatgcgtc aggcgagttg ccagatgatc agcaaggccc ggcaggacgg
     5521 cgcgcgcgtg attgccgtgg gatccgatgc atcggacaca ccggaaccgt atctgcgggc
     5581 cggcgccgac acggtgctgt ttggtgaagg tctgcaatcg ctgcaagcgc tgagcgaacg
     5641 actggatacg aatccgagtc tcccgatatc aagcctcatt gagggtctgg gggatctggc
     5701 ggcagtgctc ggcggccagc tgaaagtctt caagaacgcg cacccctcgc cttcccgtcg
     5761 gcagatggat ccgccggcct gggacctgat cgacatcgag cgctatcgca ccgtctggca
     5821 gcgcgcgcat gggttcttca gcctgaacat ggcggcctca cgcggttgct cgtttcgttg
     5881 caac
//
LOCUS       DATMRM010000437        14589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1837882, whole genome shotgun sequence.
ACCESSION   DATMRM010000437 DATMRM010000000
VERSION     DATMRM010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14589
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837882"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VNZ06_13235"
     CDS             <1..216
                     /locus_tag="VNZ06_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21759.1"
                     /translation="GQSLLTEGMFRDRHALAAFAADLRALQSNPSDAALLWKYGLGES
                     MRVEALRLLEMRNWLETEVQPRSKRHG"
     gene            complement(329..1117)
                     /locus_tag="VNZ06_13240"
     CDS             complement(329..1117)
                     /locus_tag="VNZ06_13240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWW21760.1"
                     /translation="MNRSVTRALIAALSCVALSSAFAGIALAQDPSASASAPKRSAPP
                     PKTMSDEQKTLYALGALLSRNLNAFSLTDAEFATVRQGFSDGFHHKPETKDAESAIPQ
                     VQALQKDRAQKVSDAYLAKAEKAPGAKKTASGLIFVSVKEGSGPSPTRSDNVKVSYEG
                     RLTDGTVFDSSKSAPGGTASFQLSRVIACWTEALQLMKVGGKARIVCPSSIAYGQRQM
                     GPKITPGSTLEFDIELLDIEAPPAAPAGSSSGAATGAPSPPVSH"
     gene            complement(1217..1666)
                     /locus_tag="VNZ06_13245"
     CDS             complement(1217..1666)
                     /locus_tag="VNZ06_13245"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HWW21761.1"
                     /translation="MKTESEINVFVNGVLHYFNTTVQQTAECGTPHLAIGRYPDVSDY
                     TGMIKISGKRTGMVFFTAPKGMLSVMLMRMQETDMSHDNLCDLVGEIANTLSGNARRD
                     FGHQFNISVPSVVHGRGTAVEYPEKTRPIIVPIEWRTYHARLVVCLD"
     gene            complement(1674..2048)
                     /locus_tag="VNZ06_13250"
     CDS             complement(1674..2048)
                     /locus_tag="VNZ06_13250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW21762.1"
                     /translation="MNAPAHRLMVVDDSNVIRRRIERAQDLPWVEFVGAARNGREALE
                     LHEKLKPTLVTMDLTMPEMDGGECVAHLVKRDPSIKILVISALADKVTAIDAIEKGAS
                     GFLCKPFTDRQLNEALRKLVGG"
     gene            complement(2054..4252)
                     /locus_tag="VNZ06_13255"
     CDS             complement(2054..4252)
                     /locus_tag="VNZ06_13255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW21763.1"
                     /translation="MNRGQWVQIGATVMFATVLTLLLVLGIRRASELQSASAALQLAS
                     ELSSRPELMRSELTLIQRGLETQTYIGQSLRNLASMRETSNQSYNLLQDDLKAAKLDG
                     DTAISQPLRQAFDRWQTLDEQLTALGKMRETELYTDSANASDLSAPGKKLKAAVDDML
                     VKETPNMKTMSESLGQLGVALRTAVSDSGRNLRSLLLAGTLVAALLLGAMLYYGYRST
                     KSAAEALEAQRQVENILGTVREGLFLIGRDLKLGSTCSGSLKDLLHIENPAGQTFEEV
                     LRTLVDEKTTKAALKFLGLLWRDKVHEDLIESINPLNQVEVAFAAPTGGTDTRYLAFS
                     FRRVRGDDTSGDYILGVVGDVTDRVLLARELEQVKADNDSQAAVLLQILKVDPQQMQS
                     FLTNADVAFRKSNAMLTAPGKEQEDLKKKLNGVFRELHAAKGEAASLGLSSFVQRIHA
                     AEDYLSTLRNKAQLNGNDFMPVVVKLDELMSHAASIHGMQDRVAGFRVTPQAAGADKG
                     KKGKAKDKDKPAGAGDTTEVQSQAEAEHMPIGPSIDSQLQTLSQDVSLSQSRPVKLVT
                     KGLDRVPARLAGTVKDICIQMIRNSIMHGIESPEARRDAGKLEEGTLQVTFTEDGDQE
                     FLLTIEDDGRGLNYEQIIDKALKQGMVKPQQAMTLERAAVYKLIFQPGFSTASEVSEH
                     AGRGVGLDAVSNWVRENGGNIAVSTVSGQYTRFKVVLPKTASTVLSSTAA"
     gene            complement(4302..6035)
                     /locus_tag="VNZ06_13260"
     CDS             complement(4302..6035)
                     /locus_tag="VNZ06_13260"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWW21764.1"
                     /translation="MRPRRVDVVVVSGDDEFLIEIGPLFSEGFRSRPVDSPAAIATVL
                     AAQGEDSPPTMIMLDAAAVGDPRAAVAQMEAAHPNLPIIVAAASRDESYWSAAIARGA
                     IIDVVARYDLGTDKFKDVLNRAEARARSAPQSAPPGEVTPPAKNNSKLLIAAAVAVVL
                     AAGAFFALHKSGSSGPTAPTAAVSAQQSSAASATKPMSTIELLSAARIAFRDQKLLPR
                     DGESHGDSALELYSQVLAQEPKNDEAIDGMQRLFSVIKSRVLADLASNKLDDAQRLIG
                     AFKATNVESDGVKELDAQVAAARPKFYAARAQEMIQANDFTAADAMITQLATLDRKSA
                     DELRRTMESRKLEQATQAQLTQLAASVKQAIDAGNLLEPANDSARSRLSAMRQVSRNH
                     PLTMSAQRDLQAALINRAQEQSNKDQFDAASKLIAAAGEVAATAEVAEAKKQLQGQID
                     AANQRAAAAAAARKAAEAAQAAAKQQDAAASAAAAAGPAYIAARPTTPLRVVYPSAAA
                     DNRVQGYVIVEFMLQADGRPAQPSIAESNPARIFDAAAIDAVMGAKYDTSKLTDKQPR
                     RARVRLTFKPS"
     gene            6396..8330
                     /locus_tag="VNZ06_13265"
     CDS             6396..8330
                     /locus_tag="VNZ06_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWW21765.1"
                     /translation="MRAVVLSYLMDAVLLFGFALAGTIPMPIPLAYLAAGLADSGWFY
                     LLSRRMPEGRSQWHTLTCPRMFISSAIQLTFIALVPQIGFYFVTVLFIVYGFGSMALS
                     VWQSVATWSSVALVTAVLLANGAQAAWIPQANLTERLLVWLCFISVLGRCVLLGVFGR
                     SLRERLQRRSRQLTESVGALKQRDQSLERVNAELQYQATHDALTGLANRVLFAEELEK
                     AIAAQRPFAVCVLDLDRFKVINDSLGHGAGDALLKLVGRRLLSTTRTDDAIARAGGDE
                     FLLLLQELGAREDVEKLAARWMSALSEPYRVHGTELHISPSIGIARYPIDSADGEELL
                     ARADEAMYQAKQAGRNMFRFFDADVMGSSRERLAIEADLRQAISQSQFQLHYQPKIDI
                     ASGMTRSVEALLRWQHPIRGRMMPGDFIAIAEDTGLILPIGDWVVREACHQARLWQQQ
                     GLPFLRVAVNVSPTQFRQSGFPNMVREALQAYALDPSYLEIELTEATLMSNAERSVTM
                     LEQLSRLGVLVSIDDFGTGYSSMTYLQRFPIDKLKIDRSFIRDLDSNPDDASIVRAII
                     SLAHGLRLKVVAEGVETSAQLDILRRMGCDQYQGYLRSPAVSASDIPALLAAPQISEA
                     SPAADQTFSKLARLVRSRPQ"
     gene            8591..10531
                     /locus_tag="VNZ06_13270"
     CDS             8591..10531
                     /locus_tag="VNZ06_13270"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWW21766.1"
                     /translation="MLGIAASYVTDTLLLLGFTLTGTITLSVPCCYLAAGLAECLLFH
                     HWTKRSLPLSDPQNVDRVVLIRMSVSIAIQILFMAIAPQVGFYFVGVLFIVVGFGSMS
                     STSARAAMAWSLVSLITGLLLLRAQPTDWIAHASPGQRLVVWACIVTLLARCSLLGMF
                     ARSLRLRLENRSDQLRESIAALKRRDESLARVNAELEHRATHDALTGLPNGVLFADRL
                     REAIKDARPFAVALLDLDRFKLINDSLGHGAGDATLKLVARRLLSSTHAEDTVARSGG
                     DEFLLLMRDAKSPAYLQGLAQHCLQTLSEPLRIRGAELHLTASIGVARYPADADEGQV
                     LIARADESMYQAKAAGRNAVRFYDAQLMGLAQERLQLENELRLALTSGQLELLYQPKL
                     DIASDDMRSVEALLRWKHPVRGRLAPDVFLAVAEDSGLIQSIGSWVINEACHQAKRWK
                     AQGLPHLRVSVNVSGVQFRHPEFIGIVHEALKRDALHPSYLEIELTESALMANTEKSG
                     AVLEQLSRLGVVVAIDDFGTGYSSISCLQRFPIDKVKIDRSFIRQMQHNAGDASIVRA
                     IISLAHGLRLKVIAVGVESAAQLAILRRMGCDQYQGFYRSQAVPAADIELMIAAQRAQ
                     AANDALAEPSVSKLARLVQRQR"
     gene            complement(10528..11163)
                     /locus_tag="VNZ06_13275"
     CDS             complement(10528..11163)
                     /locus_tag="VNZ06_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21767.1"
                     /translation="MSLSAVSDLGWRLQRDLLGVSGPADPSLPAALAAGRVAAAATES
                     SDTADAGDATRHTEGGAAQHVQLFMQALFQALSSAATAPSQSPGSAGNSAAVFSAPSG
                     LTYSRGSVARYRSHAYQQGSKGLQGKIQSLISALSDPQEAPDSNVSGLQGAFNRLLQD
                     LEAGGTSIASSSFGGGATLTLRSWLLGLQHNLQQPGTTAFSAVGNMVDVVV"
     gene            complement(11254..12237)
                     /locus_tag="VNZ06_13280"
     CDS             complement(11254..12237)
                     /locus_tag="VNZ06_13280"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HWW21768.1"
                     /translation="MGGRSAALILGGIALSVGAPLPAAEAPPPPGLTQLEEISVTGER
                     PGPRLWKVSREDHVLWLLGTLTHIPRKMTWRSTQVETALGSSQELLDSGFSISASIGP
                     ISAVRLYFQFRHTERTPEHTTLKTWVPPPLYARFEQVKSNFDNSDRSIEDLRPSAAAL
                     RLYDKAVDAAGLTERETVEQQVVRLARKLRVPVQRPKFELTNPSDTLKQVGDLSPALE
                     VDCLDATVTRIETDLQNMQQRALAWSVGDVDRLRALPFPNQREVCINAISNAPRIKEM
                     VDRAAQLWMDEAEAALRRNRVTFAMRPIYDLLDVRGPLAKFRAEGYSVEGP"
     gene            complement(12213..12749)
                     /locus_tag="VNZ06_13285"
     CDS             complement(12213..12749)
                     /locus_tag="VNZ06_13285"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWW21769.1"
                     /translation="MKRVMALLWAGSCAGTALAALPNGASAPDFTTQATLAGKPFQFS
                     LADALKTGPVVLYFYPAAFTSGCTVEAHEFAEATDQFKSLGATVIGVSHDDIEKLNKF
                     SVSECRNKFAVAADPDQHIMKEYDAVLKLAPSWANRTSYVISPEGKVLYSYTALNPDK
                     HVENTLAVVKQWAADRPH"
     gene            complement(12854..13753)
                     /locus_tag="VNZ06_13290"
     CDS             complement(12854..13753)
                     /locus_tag="VNZ06_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21770.1"
                     /translation="MAMISGLRWSGLCSLLAVGMISAPSPGLARSCDERCLAGIMQQY
                     LQQMPRHEPGKLPLAASINARENSEALALGQGSWKSVDGVLPGLLFADASTGEVVYAG
                     GAKRAGRIGSLFVRLKTVGGKITESELWTRGGEPTAQGGDSGRDMAGLAEPDILYEAV
                     VPSERRSSREQLIAIVRGYLEGISKHDGSIPHFSYRCDRYNAGFKWTNNPDNPESRGG
                     GTCASSFTNLTGQPVVNVRIPVVDVAHGVVAALFIIPHPERKTPGATYVAEVFKIVDG
                     KVRSIDEFGGPGGYPPNSGFADH"
     gene            complement(13786..>14589)
                     /locus_tag="VNZ06_13295"
     CDS             complement(13786..>14589)
                     /locus_tag="VNZ06_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21771.1"
                     /translation="RRDQAMKRKTGCGLFAVSLLSGAQALAASSPAPPDWSGLWHVKG
                     SVALISAPEGRMFVRGTADDAPLKPQYLAQYKADLVRALRQGDPQGVDVLTDSNTLHC
                     FAGMPRFVATPFQYEFINTPKETYIIVDKAVRHVFTDGRPWPPQDARWPLMIGRSRGH
                     WEGGTLLIETINMRDDMWLDTTPLTVSTQATVFERLRRIDPNTIENQVTIRDPVKFTH
                     DWSFTRQYVRRESTEWPDDPELCGGPEDRNPIVNGRVTTQVPSDLEPKK"
BASE COUNT         2506 a         4682 c         4561 g         2840 t
ORIGIN      
        1 ggacagagcc tgctcacgga gggcatgttc cgcgacaggc acgcactcgc agcgtttgcg
       61 gccgacctgc gcgctttgca gtcgaacccc tcagatgcgg cgctgctttg gaaatacggc
      121 ttaggggagt cgatgcgcgt cgaggcgctg cggcttctgg aaatgcgcaa ctggctcgag
      181 actgaagtgc agcctcgctc aaagcggcat ggctaacagc acctgggcgc agctcaaggg
      241 gccgcgccca ggttcgatga gttactcgaa gtaattacga tatcaaattg ataaatatac
      301 tgatttatcc cgctttcctt cggacctgtc agtgactgac tggcggactg ggtgccccgg
      361 tcgccgcacc cgagctgctg ccggctggcg ctgcgggcgg tgcctcgatg tcgagcagct
      421 cgatatcgaa ttccagcgtc gagccgggcg tgatcttcgg tcccatctgg cgctggccgt
      481 aagcaatcga cgacgggcag acgatgcgtg ccttgccgcc cactttcatc agctgcaggg
      541 cttcggtcca gcaggcgatg acgcggctga gctggaagga tgcggtaccg ccgggtgcgc
      601 tcttggaact gtcgaatacc gtgccgtccg tcagcctgcc ttcatagctt accttgacgt
      661 tgtcagagcg cgtcggactt gggccgctgc cctccttcac ggaaacgaag atcagtccgc
      721 tggcggtctt cttcgcgcca ggcgcctttt cagccttcgc cagatacgca tcgctcacct
      781 tctgcgcccg gtccttctgc agcgcctgga cctgcggaat cgccgactcg gcatccttgg
      841 tttccggctt gtgatgaaaa ccgtcggaaa acccctgccg gaccgtcgcg aactccgcat
      901 cggtgaggga aaacgcattc aggttacggc tgagcagcgc accgagcgcg tagagagttt
      961 tctgctcatc gctcatggtc ttcggtgggg gcgctgaccg ctttggggcg ctagccgagg
     1021 cgctcggatc ctgcgccagg gcaatgcctg cgaaagcgct actgagggct acacagctca
     1081 gcgcggcgat gagcgcgcgc gtcacggatc ggttcataca acactcctga atcgggggcg
     1141 cgcatgatac caaggatgca tgaaatgacc ggcacgaggc cggtcatttc gctacggcca
     1201 ggaagctttg gaagcctcag tcgaggcaga ccaccaggcg ggcgtgatag gtacgccact
     1261 cgatcggaac gatgatcggc cgggtctttt ccggatactc gaccgcagtg ccacgcccgt
     1321 ggaccaccga gggcaccgag atattgaact gatggccaaa gtcgcgccgg gcgtttccgg
     1381 agagcgtatt ggcgatttct cccaccaggt cgcacaggtt gtcatggctc atgtcagttt
     1441 cctgcatgcg catcagcatg acgctgagca tccccttcgg agcggtaaag aacaccatgc
     1501 cagtgcgctt gccgctgatc ttgatcattc cggtgtagtc cgagacgtcc gggtagcggc
     1561 caatcgccag atgcggtgtg ccgcactctg cagtctgctg cacggtcgta ttgaagtagt
     1621 gcagcacacc attgacgaac acattgattt cggattctgt tttcatctct gtctcagccg
     1681 cccacaagct tgcgcagtgc ttcgttgagt tgccgatcgg tgaagggttt gcacaggaag
     1741 ccactcgcgc ccttctcaat cgcgtcgatg gcggtgacct tatcggccaa agccgagatc
     1801 accagtatct tgatggacgg atcgcgcttc accaggtgcg cgacacactc gccgccatcc
     1861 atttctggca tggtcaggtc catcgtgacc aatgtgggct tgagcttctc gtgcagttcg
     1921 agcgcttcgc gaccgttgcg cgccgcgccc acgaattcca cccatgggag atcctgggcg
     1981 cgttcaatgc gccggcggat cacattcgag tcatccacaa ccattagccg gtgtgctggt
     2041 gcgttcatga ttcttacgct gccgtcgagg acagaacggt gctggccgtc tttggcaaca
     2101 ccaccttgaa tcgcgtgtac tgaccggaga ccgtcgaaac ggcgatgttg ccgccgttct
     2161 cgcgcaccca gttgctgacc gcgtccagac ccacgccgcg accggcgtgc tcggacacct
     2221 cgcttgcggt cgagaagccc ggctggaaga tcagcttgta caccgcagcg cgctccaggg
     2281 tcatcgcctg ctgcggcttc accatgccct gcttcagagc cttgtcgatg atctgctcgt
     2341 agttcaagcc acggccatcg tcctcgatcg tcagcaggaa ttcctggtcg ccgtcttcgg
     2401 tgaacgtcac ctgcagcgtg ccctcctcga gcttgccggc gtcgcggcgc gcttcggggc
     2461 tttcgatgcc gtgcatgatc gagttgcgga tcatctgaat gcagatgtcc ttgactgtcc
     2521 cggcgagccg cgcgggaacc cggtcgagtc ccttggtgac gagtttgacc ggacgcgact
     2581 gcgacagcga cacgtcctgg ctcagggttt gcagctgcga atcgatcgac gggccgatcg
     2641 gcatgtgctc cgcctcggcc tgagactgca cttcggtcgt atcaccggca ccggcgggtt
     2701 tgtctttgtc cttggccttg cccttcttgc ccttgtcagc gccggccgct tgcggggtga
     2761 cgcggaagcc agcgacacga tcctgcatgc cgtggatgct ggccgcatgg ctcatcagct
     2821 cgtcgagctt cacgaccacg ggcatgaagt cgttaccgtt gagctgcgcc ttgttacgca
     2881 gtgtcgagag atagtcttcg gcagcgtgga tgcgctgcac gaagctggac aggcccagcg
     2941 aggccgcttc gcccttggcg gcatgcagct cgcggaacac gccgttgagc ttcttcttga
     3001 ggtcttcctg ctccttgccc ggcgccgtca gcatcgcgtt gctcttgcgg aacgcgacgt
     3061 cggcgttggt gaggaacgac tgcatttgct gcggatcgac cttgaggatc tgcagcagca
     3121 cagcggcctg cgaatcgttg tcggccttga cctgttcaag ctcgcgcgcc agcagcacgc
     3181 gatcggtcac gtcgcccacg acacccagga tgtagtcgcc cgaggtatca tcgccacgca
     3241 cccggcggaa cgagaacgcc agatagcgcg tgtcggtgcc accggtcggt gcggcaaagg
     3301 ccacttcgac ctggttcaga ggattgatcg actcgatcag atcttcgtgc accttgtcac
     3361 gccacagcag gccgaggaat ttcagcgccg ccttggtggt cttctcatcc accagcgtac
     3421 gcagcacttc ctcaaaggtc tgacccgccg gattctcgat gtgcagcagg tccttcagcg
     3481 agccggagca ggtcgaaccg agcttcaggt cgcgtccgat cagaaacagt ccttcgcgca
     3541 ccgtacccag aatgttctcg acctggcgct gggcttccaa cgcctcagcg gccgacttgg
     3601 tcgaacgata accgtagtag agcatcgcac cgagcagcag cgcggcaacc agggtaccgg
     3661 ccagcagcag cgagcgcagg ttgcggccgc tgtcgctgac tgcggttcgc agtgcgacgc
     3721 ccagctggcc caggctctcg gacatggtct tcatgttggg tgtctctttc accaacatgt
     3781 catccacggc ggccttcagc ttcttgccgg gtgcactgag gtctgaggca ttggccgagt
     3841 cggtgtagag ctcggtttcg cgcatcttac cgagcgccgt gagctgctcg tccagcgtct
     3901 gccagcgatc gaacgcctgg cgcagcggct gagagatggc ggtgtcgccg tcgagcttcg
     3961 cggccttcag atcatcctgc aacaggttat aggactgatt gctggtctcg cgcatcgagg
     4021 cgagattgcg cagcgactgc ccgatgtagg tctgcgtctc cagtccgcgc tgaatcagcg
     4081 tcagctccga gcgcatcagt tccggccggg aactcagttc cgacgccagc tgcagcgcgg
     4141 ccgaagccga ctgcaactcg gatgcgcggc ggataccgag caccagcagt agggtcagta
     4201 cggtggcgaa catcacggta gcaccgatct gcacccactg tccgcgattc attgttaagc
     4261 gtcgagccat tcggattctt cctgccgtta gctgatttct attaactggg tttaaaggtc
     4321 agacgcacgc gcgcgcggcg cggctgtttg tcggtcaact tgctggtgtc gtacttggca
     4381 cccatcaccg cgtcgatcgc agcggcatcg aagatgcgcg ccgggttcga ctccgcaatg
     4441 ctcggctgcg ccggccggcc atccgcctgc aacatgaatt cgacgatcac gtagccctga
     4501 accctgttgt ccgcggccgc tgacggatac accactcgca gcggcgtggt cggccgcgca
     4561 gcgatgtatg ccgggcccgc cgccgccgcc gctgaggccg ccgcgtcctg ttgcttggcc
     4621 gcggcctggg ccgcttcggc cgccttcctg gccgccgcgg ctgccgcagc gcgctgattg
     4681 gcggcatcga tctggccctg cagctgtttc ttggcctcgg cgacctccgc ggtcgcagcc
     4741 acctcaccgg ccgccgcaat cagcttcgag gcagcatcaa actgatcctt gttgctctgt
     4801 tcctgcgcgc ggttgatcag tgctgcctgc agatcgcgct gggcgctcat ggtcagcgga
     4861 tgattgcgcg acacctgacg catcgcgctc aggcggctgc gggcgctatc gttcgcgggc
     4921 tcgagcagat tgccggcgtc gatggcctgc ttgaccgagg cggccaactg cgtgagctgc
     4981 gcctgggtcg cctgttcgag cttgcgcgat tccatcgtgc gccgcaactc gtcggcggac
     5041 ttgcgatcga gcgtcgccag ctgcgtgatc atcgcatccg cagcggtgaa gtcgtttgcc
     5101 tgaatcatct cctgcgcgcg cgccgcatag aacttcggtc gtgccgcggc gacctgcgcg
     5161 tccagttcct tcacgccatc ggattcgaca ttggtggcct tgaacgcgcc gatcaggcgc
     5221 tgcgcatcgt cgagcttgtt ggaggcaaga tccgccagga cgcgcgattt gatcacgctg
     5281 aacagacgct gcatgccatc gatggcctca tcgttcttcg gctcctgcgc cagcacctgg
     5341 gaatacagct cgagcgcgct gtcgccatgc gactcgccat cgcgcggcag cagcttctga
     5401 tcgcggaatg caatgcgggc agccgacagc agctcgatcg tcgacatcgg cttggtcgcc
     5461 gaagcggcag aggactgctg agcgctgacg gccgcggtcg gagctgtcgg accgctcgat
     5521 ccggacttgt gaagtgcgaa gaaggcccct gccgccagca ccacggccac ggccgctgcg
     5581 atcaggagct tgctgttgtt cttggccggc ggcgtcacct cgcccggcgg cgcactttgc
     5641 ggtgcactgc gggcgcgggc ctcggcgcga ttgagtacgt ccttgaactt gtcggtgccc
     5701 aggtcgtagc gggcgaccac atcgataatg gcgccgcgtg cgatcgccgc gctccaatac
     5761 gactcatcac gtgatgcggc cgccacgatg atcggcaggt tcgggtgtgc ggcttccatc
     5821 tgcgccacgg cagcgcgcgg atccccgacg gctgccgcat ccagcatgat catggtcggc
     5881 gggctatcct cgccctgtgc ggccagcacc gtggctatgg cggccggcga atcgactggg
     5941 cggctacgga agccctcgct gaacaggggc ccgatctcga tcagaaactc gtcatccccc
     6001 gagacgacca cgacatccac tcggcgcggc cgcatacgcg tcggttctac tgcgggaatc
     6061 gggtcgttcg caacgggcat catcgggcta taccttgtgc gtgagggaac acgtgccgcg
     6121 aactctggtg ttacagagga aaagcgacag cgcatcggtt cacattttgt aagctcgcga
     6181 tctcggcact ggcgtcgccc tttcgctgat gagcgccagg tcacataatt caccccaccc
     6241 ccggtcgccg tttcgatgtg catcacgtca cggcagctgc ccagacaggg gcgtaaggtt
     6301 tccggcttct atcgctatgt ctgcggcggg ccgggtcccg gatggaacag atcgctcgtg
     6361 ggcagactcc cacgcgtcag ttgggtatcg ggacaatgcg cgccgttgtc ctcagctacc
     6421 tgatggacgc tgtcctgctg ttcggttttg ccctggccgg taccatcccg atgccgattc
     6481 cgctggccta cctggcggcc gggcttgccg attcagggtg gttctatctg ctgtcccgcc
     6541 gcatgcccga gggtcgcagt cagtggcata ccctgacctg cccgcgaatg ttcatctcga
     6601 gcgcgatcca gctcacgttc atcgcgctgg tcccgcagat cggcttctat ttcgtcaccg
     6661 ttctgttcat cgtttacggc ttcggctcca tggcgctgag tgtgtggcag tcggtggcga
     6721 cctggagcag tgtcgcgctc gtgaccgccg tgctgctcgc caacggcgcg caggcggcct
     6781 ggattccgca ggccaacctc accgaacgct tgctggtgtg gctgtgcttc atatcggtac
     6841 tgggccgctg cgtgctgctg ggtgtgttcg gtcgctcgct gcgcgagcga ctgcaacggc
     6901 gcagccggca gctcaccgaa tcggtcggcg ccctgaagca gcgcgatcaa tcactcgagc
     6961 gtgtcaacgc ggagctgcag tatcaggcca cccacgatgc actgaccgga cttgccaacc
     7021 gagtgctgtt cgccgaagag ctggagaaag caatcgccgc gcagcggccg ttcgcggtat
     7081 gcgtactgga cctggatcgg ttcaaagtca tcaatgactc gctcggtcac ggtgcaggag
     7141 atgcgctgct gaaactggtc ggccggcgac tgctgtccac cacgcgcacc gacgatgcga
     7201 tcgcgcgcgc gggcggcgat gaattcctgc tgctgctaca ggagctgggt gcccgcgaag
     7261 acgtcgagaa actggcagct cgctggatga gcgcgctgtc ggaaccctat cgcgttcatg
     7321 gaaccgagct gcacatctcg ccgagcatcg gcatcgcgcg ctacccgatc gattcggccg
     7381 atggtgagga actgctggcg cgggccgatg aggccatgta tcaggccaaa caggccggcc
     7441 gcaacatgtt tcgcttcttc gatgcggacg tgatgggatc ctcgcgcgag cgtctcgcga
     7501 tcgaggctga cctgcgccag gccatctcgc agtcgcagtt tcagctgcac tatcagccga
     7561 agatcgacat tgccagcggc atgacacgca gcgtcgaggc gctgctgcgc tggcagcacc
     7621 cgatccgtgg ccggatgatg ccgggcgact tcatcgcaat cgccgaggac accggactga
     7681 tcctgccgat cggggactgg gtggtacgcg aggcgtgtca ccaggcgcgg ctctggcagc
     7741 agcagggcct gccgttcctg cgcgtcgcgg tcaatgtctc gccgacgcaa ttccgccaga
     7801 gcggctttcc aaatatggtt cgcgaagccc tgcaagccta cgcgctcgat ccgtcgtatc
     7861 tggagatcga gctgaccgag gcgacactga tgagcaacgc cgagcgctcg gtgacgatgc
     7921 tcgaacagct cagccgcttg ggggtgctgg tgtcgatcga tgacttcggc accggctatt
     7981 cgagcatgac ctatctgcag cgcttcccga tcgacaaact gaagatcgac cgcagtttca
     8041 tccgcgacct ggatagcaac cccgatgatg cctcgatcgt acgcgccatc atctcgctgg
     8101 cacacggtct gcggctgaag gtcgtggccg agggcgtcga aaccagtgca cagctcgaca
     8161 tcctgcgacg catgggctgc gaccaatacc aggggtatct gcgcagcccc gctgtgagtg
     8221 cctcggacat tccggccctg ctggcggcgc cgcagatctc ggaggcttcg cccgcagcgg
     8281 accagacctt ctccaagctt gcgcgactgg ttcgctcccg accgcaatga gcgcagcccc
     8341 ggtggaaatt gaaggctcga tgaaggccgc tccggcaccg gcgctgttga cctgccgcgc
     8401 aaccgaactt tcagcaaact tgccagactc ttacgagcta aaccttagct aatttagcat
     8461 ctagctgtgg agcgcgtcac agtgctgcgt gtcatccggg cgtaacgttc ctttccgctt
     8521 cctttcaacc cgcgacgacc gcgcccgcgc catgaggact tccgttggct tagcaaacag
     8581 ctcccgcgtg atgcttggca tcgcggcaag ctacgtcacc gacacgctgt tgctgctggg
     8641 attcacgctg accggaacga tcacgctgtc ggtcccctgc tgctatctgg ccgccgggct
     8701 tgccgagtgc ctgctgttcc atcactggac caaacgatcg ctgccgctga gcgacccgca
     8761 gaatgtcgat cgcgttgtgc tgatacgcat gagcgtgtcg atcgcgatcc agatcctctt
     8821 catggccatc gcgccgcagg tcggtttcta tttcgtcggc gtgctgttca ttgtcgtcgg
     8881 cttcggttcg atgagttcga cctccgctcg cgctgccatg gcctggtcgc tggtgtcgtt
     8941 gatcactggc ctgctgctgc tgcgggcgca gcccacggac tggattgctc acgccagccc
     9001 cgggcagcga ctggtggtgt gggcctgtat cgtgaccttg ctggcccgat gcagtctgtt
     9061 ggggatgttc gcgcgctcac tgcggctgcg actggaaaat cgcagcgatc agctgcgcga
     9121 atcgatcgcg gcgctcaagc ggcgcgatga atcgctggcc cgcgtcaacg ccgagctgga
     9181 gcatcgcgcc acgcacgacg ccctcaccgg ccttccgaac ggcgtgctgt tcgccgatcg
     9241 cctgcgcgag gcgatcaagg atgcccgtcc cttcgcggtc gcgctgcttg acctggatcg
     9301 attcaaactg atcaacgact cgctcggcca cggcgcgggc gatgccacgc tgaagttggt
     9361 tgcgcgccgg ctgctgtcca gcacgcacgc cgaagacacc gtcgcgcgca gcggtgggga
     9421 tgagttcctg ctgctgatgc gcgacgcgaa gtcaccggcc tatctccagg ggcttgcgca
     9481 gcactgcctg cagacgctgt cggaaccatt gcgcattcga ggcgccgaac tgcacctcac
     9541 cgccagcatt ggagtggcgc ggtacccggc ggacgcggat gagggacagg tgctcatcgc
     9601 acgcgccgat gaatcgatgt atcaggcgaa ggcagcgggc cgcaatgcgg tgcgtttcta
     9661 cgatgcgcag ctgatgggac tggcgcagga gcggttgcag ctcgagaacg agctgcggct
     9721 cgcgctgacc agcggtcagc tcgaactgct ctatcagccc aagctggaca tcgccagcga
     9781 cgacatgcgc agtgtcgaag cgctgctgcg ctggaagcac ccggtgcgcg ggcggctggc
     9841 accggacgtc tttctggccg tggcggaaga ttccggcctc atccaatcca ttggctcctg
     9901 ggtaattaac gaagcctgcc atcaagcgaa acgctggaag gctcaggggc tgccgcacct
     9961 gcgtgtctcg gtcaacgtgt ccggcgtaca gtttcgacat cccgaattca ttggcatcgt
    10021 gcacgaagcc ctcaagcgcg acgccttgca tccgtcctat cttgaaatcg agctgaccga
    10081 atccgcgctg atggcaaaca ccgaaaaatc gggcgcagtg ctggaacaac tcagccgcct
    10141 gggcgtcgtg gtcgcaattg atgatttcgg caccggctac tcgagcataa gctgtctgca
    10201 gcgattcccg atcgacaagg tcaagatcga tcgcagcttc atccgccaga tgcagcacaa
    10261 cgccggcgac gcatcgatcg tgcgcgcgat catctcgctg gcgcacgggc tgcgtctcaa
    10321 ggtgatcgcg gtaggggtcg aatcggccgc gcagctcgcg atcctgaggc gcatggggtg
    10381 cgatcagtat caggggttct accgcagtca ggcggtaccg gccgctgaca tcgagctgat
    10441 gatcgccgcc cagcgcgcgc aggccgcgaa cgacgcactg gcggaaccga gcgtcagcaa
    10501 gctcgcgcga ctggtccagc gccagcgcta gacaacgacg tccaccatgt tgccgaccgc
    10561 cgagaatgcg gtcgtacctg gttgctgcag gttgtgctgc aatccgagca gccagcttcg
    10621 cagcgtcagt gtcgcaccgc cgccgaagga actcgacgca atgctcgtac cgccggcttc
    10681 caggtcctgc agcagtcgat tgaaggctcc ttgtaatccg ctgacgttgc tgtccggcgc
    10741 ctcttgcgga tcgctcaggg cactgatgag tgattgaatt ttgccctgca gtcccttcga
    10801 gccctgctga taggcatggg agcgatagcg ggcgaccgat ccgcgcgaat aggtcagacc
    10861 cgagggagca ctgaagaccg ccgcgctgtt gccggcgctg ccgggggatt ggctgggcgc
    10921 ggtcgctgcg ctcgacagcg cttgaaacag cgcctgcatg aagagctgca catgctgcgc
    10981 ggcgccgccc tcggtgtgac gggtcgcatc gcccgcatcg gcggtatcgg agctttcggt
    11041 cgcggctgca gccacccgac cggcagcaag ggccgcagga aggctcgggt cggcggggcc
    11101 ggacacaccc agcaggtcac gctggagacg ccatcccagg tcggagactg cggataacga
    11161 cattgaagca gtctaacagt ccgcggcaag gatggggtgc gcccgctcgg cgctaaaagt
    11221 gcgaaccagt gccgggattg atcaaaccgc gctctaagga ccttcgacgc tgtaaccttc
    11281 cgcgcgaaat ttagcgagcg gcccgcgaac atccagcagg tcgtagatcg ggcgcatcgc
    11341 aaaggtgacg cgattgcggc gcagagccgc ttccgcttcg tccatccaca attgcgccgc
    11401 acgatccacc atttccttga tgcgcggcgc gttcgagatg gcgttgatgc acacctcacg
    11461 ctgattcgga aagggcaacg cgcgcaaccg atccacatca ccgactgacc aggccagcgc
    11521 tcgctgctgc atgttctgca agtcggtctc gatacgggtg accgtcgcat caagacaatc
    11581 gacttcgagg gcgggcgaga ggtccccgac ctgcttgagc gtatcggacg gattggtcag
    11641 ctcgaacttc ggtcgctgca ccggcacgcg cagtttcctg gcaagcctca ccacctgctg
    11701 ctccacggtc tcgcgctcgg tcaggcctgc cgcatccacg gccttgtcat acaggcgcaa
    11761 cgccgcggca ctcggccgca gatcctcgat gcttcggtcg ctattgtcga agttcgattt
    11821 gacctgctcg aagcgcgcgt agagcggtgg cggcacccat gtcttcagcg tggtgtgttc
    11881 gggagttcgc tcggtgtgac ggaactgaaa gtacagcctt accgccgaga tcggcccgat
    11941 gctggccgaa atggaaaacc cgctatcgag cagctcctgc gaactgccga gcgcggtttc
    12001 gacctgcgtc gagcgccagg tcatcttgcg cggaatgtgc gtcagtgtgc ccagcaacca
    12061 caggacgtgg tcttcccggg agaccttcca caggcgaggt ccgggccgct cgccggtgac
    12121 actgatctcc tcgagttgcg tcagtcctgg cggaggcgga gcctcggccg ctggcagcgg
    12181 cgcgccgacg ctcagtgcta ttccgccgag gatcagtgcg gccgatcggc cgcccattgc
    12241 ttcaccacgg ccagcgtatt ctcgacgtgc ttgtcgggat tcagcgccgt gtagctgtaa
    12301 agcaccttgc cctcgggcga aatgacgtac gaggtgcggt tcgcccacga cggcgccagc
    12361 ttcagcaccg catcatattc cttcatgatg tgctgatccg gatcggcggc caccgcaaac
    12421 ttgttgcggc actcgctcac cgaaaacttg ttgagctttt cgatgtcgtc atgcgatacg
    12481 ccgatgacgg tcgcaccgag actcttgaac tgatcggttg cctcggcaaa ctcgtgcgcc
    12541 tcgacggtac agccgctcgt gaacgcggcc gggtagaaat acagcacgac cggccccgtc
    12601 ttcagggcgt cggccaacga aaactgaaac ggcttccctg ccagcgttgc ttgggtcgtg
    12661 aagtccggtg cactcgcacc gtttggcagc gccgccagtg ccgtcccggc acaggagcct
    12721 gcccatagca gggccatcac tcgtttcatc aggcgtacct cgagggtctt gggcgcaggg
    12781 acgccattat ggaggccgtc cgcaggcgga gcaatccgcg agccccgcgg ggttttgagc
    12841 gagcgaactg gacttagtgg tcggcgaaac cggagttggg tggatagccg cccggcccgc
    12901 cgaactcatc gatcgagcgg accttgccat cgacgatctt gaagacttcg gcgacgtagg
    12961 tggcgccggg agtcttgcgc tccgggtgcg ggatgatgaa aagcgcagcc accacgccat
    13021 gcgccacatc caccaccggg atgcgcacat tgacgacggg ctggccggtg aggttggtga
    13081 agctcgaggc gcaggtgcca ccgccacggc tctccgggtt gtccggattg ttggtccact
    13141 tgaacccggc gttgtagcga tcgcagcgat agctgaagtg cgggatcgag ccgtcgtgtt
    13201 tgctgatccc ctcgaggtag ccccgcacga tcgcgatcag ctgctcgcgg ctggaccggc
    13261 gctcgctcgg taccaccgct tcatagagga tgtccggctc ggccagtccg gccatatcac
    13321 gaccggaatc accgccctgc gcggtaggct cgccgccgcg ggtccacaac tctgattcgg
    13381 tgattttgcc gccgacggtc ttcaagcgaa cgaacagcga gccgatccgt cccgcgcgct
    13441 tggcgccgcc ggcgtagacc acctcgccgg tggaggcatc ggcgaacagc agaccaggca
    13501 gcacgccgtc aacgctcttc catgaaccct ggcccagcgc cagcgcctcc gagttctcgc
    13561 gcgcgttgat cgatgctgcg agcggcagct tgcctggctc atgacgcggc atctgctgca
    13621 ggtattgctg catgatgccc gccaggcagc gctcatcgca tgacctcgcc agccccgggc
    13681 tcggtgcgct gatcatccca accgccagca gtgagcacag tccactccaa cgcaagccag
    13741 atatcatcgc cacagcctct ctcctaagat cgttgatgca accgattatt tcttcggctc
    13801 cagatcgctc ggtacctgcg tggtcacgcg gccattgaca atggggttgc gatcctcggg
    13861 cccaccgcac agctccggat catcgggcca ttcggtgctc tcccggcgca cgtactggcg
    13921 cgtgaaactc cagtcgtgcg tgaacttgac cgggtcacga atggtgacct gattctcgat
    13981 ggtattggga tcgatcctac gcagccgctc gaataccgtc gcctgcgtgg acacggtcag
    14041 cggcgtggtg tcgagccaca tgtcatcgcg catgttgatc gtctcgatga gaagcgtgcc
    14101 gccttcccag tgtccacgcg accgaccaat catcagtggc cagcgcgcat cttgcggcgg
    14161 ccagggacgg ccatcggtga agacatggcg taccgccttg tcgacgatga tgtaggtctc
    14221 cttcggcgta ttgatgaatt catattgaaa cggtgtcgcc acgaagcgcg gcatgcccgc
    14281 aaagcaatgc aaggtattcg agtcggtcag cacatcgacg ccttgcggat caccctgtct
    14341 gagcgcgcgc accagatcgg ctttgtactg cgccagatac tgcggcttca ggggcgcgtc
    14401 atccgcggtg ccgcgtacga acatcctacc ctcaggcgcc gatatcaacg cgaccgagcc
    14461 tttgacatgc cacagtccgg accaatcggg cggtgccggg cttgacgctg ccaacgcctg
    14521 tgcaccgctc agcagcgaga cggcgaacag gccgcatcct gtctttcgtt tcattgcctg
    14581 gtctcgccg
//
LOCUS       DATMRM010000438         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_584508, whole genome shotgun sequence.
ACCESSION   DATMRM010000438 DATMRM010000000
VERSION     DATMRM010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584508"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..454
                     /locus_tag="VNZ06_13300"
                     /pseudo
     CDS             306..454
                     /locus_tag="VNZ06_13300"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            483..716
                     /gene="dcd"
                     /locus_tag="VNZ06_13305"
                     /pseudo
     CDS             483..716
                     /gene="dcd"
                     /locus_tag="VNZ06_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785767.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
     gene            complement(720..1424)
                     /locus_tag="VNZ06_13310"
     CDS             complement(720..1424)
                     /locus_tag="VNZ06_13310"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HWW21772.1"
                     /translation="MLRAVAVSTLLGALCGSAHSHAAELSPFEASYAWSWHGATVAVS
                     TLKLENRGRNTWAYTSSTEPRGLGRLYPYRPRLTSLLRVTERGVEPQHFRADAGSKDR
                     DADVEFNWETGRATGTYDGVAVDLPIKDGVQDDLSVQVELIVDLLRGKTPDTLWLIDK
                     NTIRDYHYQREGDETLDTPLGHIATTIYSSQHTGSPRITRFWCAPSLGYLPMRVEQKR
                     IDSVEWTMEIRALTRN"
     gene            complement(1417..2076)
                     /gene="purN"
                     /locus_tag="VNZ06_13315"
     CDS             complement(1417..2076)
                     /gene="purN"
                     /locus_tag="VNZ06_13315"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HWW21773.1"
                     /translation="MAVLISGGGSNMAAIARACTQGLIAARIVQVIADRASAGGIELA
                     RGLGLTATLLDSGSYPTREAHELAIAQLIEHSGAGLIALAGYMRVLGASFVRRYTGRV
                     LNIHPSLLPKYKGLHTHRRALQAHEREHGASVHFVTAELDGGPLICQARVPVLPDDSE
                     QTLAARVLTHEHRIYPKAVGLIADGRIALSDDRVLLDGQPLLAPLNEEDTPAVSQARH
                     A"
     gene            complement(2100..>2840)
                     /gene="purM"
                     /locus_tag="VNZ06_13320"
     CDS             complement(2100..>2840)
                     /gene="purM"
                     /locus_tag="VNZ06_13320"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HWW21774.1"
                     /translation="VQGAEPLFFLDYYATGRLNVDVAATVLSGIVEGCAQAGAALVGG
                     ETAEMPGMYHGDDYDLAGFCVGVVEKEAIIDGRRVNAGDAIIALASSGPHSNGYSLIR
                     KLIAVAGATSQTQLEGRGLYEQLLTPTRIYVKSLLALIKTMPVHGLAHITGGGLTDNI
                     PRVLRDGLEARLQRVRWPEMPIFDWLARTGAIAPAEMYRTFNCGIGMIAIVAPERAEE
                     AVRCLSQHGEQAMLIGEVQPGARGVVIE"
BASE COUNT          541 a          880 c          892 g          527 t
ORIGIN      
        1 gatttggcat cttctcgctg atccgcgaga agataacgag catcggtacg caagcatgca
       61 agattcagat cactgacaag gatcagatcc agcgttttgc ggagcgaatt ggattttcac
      121 ccgggtgcgc gaaacaacaa cggctggaaa cgcagatcct gccgatgatt cagtcgcagc
      181 ccaaggtgaa gaacaacttc gatacgctgc ctgccgaggc gtgggcgatc gtcagcgctg
      241 cagctagagt gggcggcgtt tcgctgagat ctgtcggcgt tgccgccagc aagcagtcag
      301 tatccatgaa ctcggcgcgg cgggtcgcat ttcggaccgg agatctctat ttggatccgc
      361 tgattgaagg tccgctgtgg gatgtcgtgg aacaaataga gcccgccggc acggaagaag
      421 tgtttgatat ttctgttccg aacctgcata acttcgttgc gaacgatttg atcgttcata
      481 attcaacgta cgcacgctgc ggtataatag tgaatgtaac tcccctggaa cccgagtggg
      541 aaggccacgt gaccctcgag ttctccaaca ccacgccgct gccggcgaag atctacgcca
      601 acgaaggcgt cgcacagatg ctgttctttg aatcggatga ggtatgtgaa acctcatacg
      661 ccgatcgcgg cggcaaatat cagaagcagc gcggcgtcac cttgccaaag gcatgatgtt
      721 cagttgcgcg tcagcgcgcg gatctccatg gtccactcga ccgaatcgat gcgtttctgc
      781 tccacgcgca tcggcaggta gccgagggaa ggagcacacc agaagcgcgt gatgcgcggc
      841 gagccggtgt gctgactgct gtagatcgtc gtggcgatgt ggccgagcgg agtatcgagc
      901 gtctcgtcgc cttcgcgctg atagtgatag tcgcggatcg tgttcttgtc gatcagccac
      961 agcgtatccg gggtcttgcc gcgcagcaga tcgacgatca actcaacctg aaccgacagg
     1021 tcatcctgca cgccgtcctt gatcggcaga tcgaccgcga caccatcata ggttccggtc
     1081 gcccgtcccg tctcccagtt gaactcgaca tccgcgtcgc ggtctttgct gccagcgtcg
     1141 gcacgaaaat gctgcggctc gacaccgcgc tcagtgaccc gcagcaggct ggtcagccgc
     1201 ggccggtagg gatacagccg gcccaatccg cgcggttcgg tgctggaggt gtaggcccag
     1261 gtgttgcggc cccgattttc gagcttgagc gtggagacgg ccacggtagc accgtgccag
     1321 ctccaggcgt aggaagcttc aaacggggat agctcggcag cgtgcgaatg agcgctgcca
     1381 cacagcgcgc ccagcaacgt ggacacggcg acggccctaa gcatgtcgtg cctgcgacac
     1441 cgcgggcgtg tcctcctcat tgaggggagc caacagcggc tggccgtcga gcaggacgcg
     1501 atcatcgctc aacgcaatcc gcccgtcggc aatcaaaccc accgctttcg gatagatcct
     1561 gtgctcgtgc gtcagcaccc gtgcggccag cgtctgctcg ctgtcgtcgg gtagcaccgg
     1621 gacgcgcgcc tggcatatca gcggtccgcc atccagttcc gcggtgacga aatgcacact
     1681 ggcgccgtgc tcgcgctcat gagcttggag ggctcgtcgg tgcgtatgca ggcccttgta
     1741 cttcggcaag agcgacggat ggatgttgag cacgcgcccg gtgtatcggc gcacgaacga
     1801 tgcgcccaga acgcgcatat agccggccag ggcgatcaga ccggcaccac tgtgctcgat
     1861 gagttgcgcg atggccagct catgcgcctc gcgcgtcgga tagctcccgg aatcgagcag
     1921 cgtcgccgtc aatccgaggc cgcgtgcgag ttcgatgcca cctgcgctgg cgcgatcggc
     1981 aatgacctga acgatccggg ccgcgatcag cccctgggtg caggcacgcg cgatcgcggc
     2041 catgttgctg ccgcctcccg agatcaatac agccacgggc agccgctcgc gtggcatcgt
     2101 cattcgatga cgacaccgcg cgcgccgggt tgcacctcgc cgatcagcat cgcctgctcg
     2161 ccgtgctggc tcagacatcg caccgcctct tcggcgcgtt cgggtgccac gatggcaatc
     2221 atgccgatgc cgcaattgaa ggtgcgatac atctcggccg gcgcgatggc accggtgcga
     2281 gccaaccagt cgaaaatcgg catctctggc cagcgcacgc gctgcaggcg ggcctcgagg
     2341 ccatctcgca gcacccgcgg gatgttgtct gtcagtccgc cgccggtgat gtgcgccagt
     2401 ccgtgcactg gcatcgtctt aatgagtgcc agcagcgatt tcacataaat gcgtgtcggg
     2461 gtcagcagct gctcatagag gccgcgaccc tcgagctgcg tctgtgaggt cgcaccagcg
     2521 accgcgatga gcttgcggat cagtgaatag ccgtttgaat gcgggccgga ggaggccagt
     2581 gcgatgatcg catcgccggc gttgacccgg cggccatcga tgatggcctc cttctcgacc
     2641 acgccgacgc aaaatccggc cagatcgtag tcgtcaccgt ggtacatgcc gggcatttcg
     2701 gcggtttcgc cgcccaccaa tgccgcgccg gcctgtgcgc agccttcgac gatgccgctg
     2761 agcaccgtgg ccgcaacgtc gacgttcagt cggccggtgg cgtagtaatc cagaaaaaac
     2821 aggggttccg cgccctggac
//
LOCUS       DATMRM010000439         6526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4618703, whole genome shotgun sequence.
ACCESSION   DATMRM010000439 DATMRM010000000
VERSION     DATMRM010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4618703"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      117..300
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            422..2314
                     /locus_tag="VNZ06_13325"
     CDS             422..2314
                     /locus_tag="VNZ06_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWW21775.1"
                     /translation="MRIQWLGSIVGVLAASASGVARADSPDFADTLQPVVVTATKIPT
                     PESQLASSVTVISAEQIAAMQATTLPDVLKYVPGLNLVQSGGPGGQTSVFMRGTNSNH
                     VKVTVDGIDVSDPGTPNGSFDFGQFLVQDIERIEVLRGPQSGLYGSDAIGGVINVITK
                     NGSGPPQLVAALEAGSFQTFNQAVAVSGSQDAFHYAVSLAHEHAGSTPVTPLDLLAPG
                     EGRRNDYDDNLTVSTKLSYVANSALDWGVVGRYTDTHLHFTGDNFNLVPAVPDANQSE
                     TDTGSYYGRVFQHLALLDGRLDQTLDVAYTRNRSDNFSLDNGESLAIGQRLKFDYQGA
                     FKIIDSQTLVFGAEHARDEISEPIDAGLSIDSGYLELQSQLAQRLFSAINVRYDSNSL
                     FGHKVTFRIAPTYVIAETGTQLRASVGTGFKAPTLSQLFQNFPPTFFANPDLKPESSI
                     GWDLGAEQALLAGTVRAGATYFHIRLRDLIDSNDTFTTNINVDKATTSGVESFISYRP
                     WAALTLRLDYTYTEATDDTTQQELLRRPKNKTSLDGNWQATAALSFNTDLLYVGPWND
                     FPRYGFATAQAPGYFTVNVAGNYKLRSNVEVYARMNNLLDRRYEDPSGFLQPAFGVFA
                     GIKARL"
     gene            2362..3126
                     /locus_tag="VNZ06_13330"
     CDS             2362..3126
                     /locus_tag="VNZ06_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWW21776.1"
                     /translation="MMSKELQAALDAARAAAEVIQGMYRRNLAVTLKADKSPVTEADV
                     KAEQAIRSVLQARFPDHGFYGEETGRHGVDAENVWLVDPIDGTKSFVRDCPFFSTQIA
                     LLRAGRLVLGVSCASAYGELAWAEEGGGAFLDGQAISVSPVSELGSAILSTGNLKTLS
                     ASATGWLRFGALVGRVNRLRGYGDFLHYHLLARGALDAVLESDVNILDIAALTVIVRE
                     AGGRFTDLSGGEVGLQTTTVLASNALLHPQLLEALS"
     gene            complement(3127..3903)
                     /locus_tag="VNZ06_13335"
     CDS             complement(3127..3903)
                     /locus_tag="VNZ06_13335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWW21777.1"
                     /translation="MNLAAHPLQATGISITAGERTLVQDLTLTVRRAEFLAILGRNGS
                     GKSLSLHALAGLNRVAEGKVLLGGEDISTMPRRTIARQLGLLLQDREEALSLSALEAA
                     LIGRHPHLKFWQREGADDLAIAQAALRRMGLGDFTRRNVHTLSGGEQRRAAMASLLTQ
                     QPRIYLLDEPTNHLDPHHQIHIMQVFRELCSQGASVIATLHDPTLAERFADRVLLLYG
                     DGRYQLGPAAQVLTAEELSALYLTPIEAIGVAPRRAFIAA"
     gene            complement(3913..4602)
                     /gene="pdxH"
                     /locus_tag="VNZ06_13340"
     CDS             complement(3913..4602)
                     /gene="pdxH"
                     /locus_tag="VNZ06_13340"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HWW21778.1"
                     /translation="MPFHREHLPEQLPAEPLSIVADWLQQATARRAQPNPNAMVLATV
                     NSQGSPAARVVLCKQIVPHPGYITFYTNYLSRKGCELSANPRAAAVMHWDAMHRQVRL
                     EGIVSRSSSAESDAYFSGRSWQSRIGAWASQQSAPLLSRSDLERAAQRMAQRFGTPSP
                     ISSDDTAPDPGVPIDRPPHWGGFHLWIDGCELWVEGAGRLHDRARWTRVLSLEQGIYQ
                     GGPWQAIRLQP"
     gene            4632..5783
                     /locus_tag="VNZ06_13345"
     CDS             4632..5783
                     /locus_tag="VNZ06_13345"
                     /inference="COORDINATES: protein motif:HMM:NF042869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HWW21779.1"
                     /translation="MHATKLPFALQVLVDMTLDELRRHLDELDERLLQIIAQRQSTSR
                     EIARVKRSTGVPTRDYGREREVILGARGQAMKIGVSPDLAETFMRLLIRSSLTTQEQA
                     SVVAHGAGSGQRALLIGGAGKMGAWFASFLTSQGFDVQIADPRAMSGPNVIADWHDSA
                     LTQDYIVVAAPLGATNQILQQLAERKPRGVVLDLGSLKSPLRAGLEALRAAGIMVTSI
                     HPMFGPDTELLSGRHVIFIDLGNAEALARARTLFAPTMAEQVVMGLDEHDRLIAYVLG
                     LSHALNIAFFTALAESGEAAPRLARLSSTTFDAQFDIASKVAEESPELYFEIQRLNDF
                     GAESLNALARAVEAVRSAVFDNDQARFTALMRQGNEYVKDRRDSAARRV"
     gene            complement(5796..>6526)
                     /locus_tag="VNZ06_13350"
     CDS             complement(5796..>6526)
                     /locus_tag="VNZ06_13350"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWW21780.1"
                     /translation="FRNPLADPLVLGVSGGAAIGALLAMLTGADLAVMHGYATAGGTL
                     IGLSVLLMARGGGTTRLLLCGVVLASACGALITVLLTLADNGQLRGMLFWLAGDLGWA
                     QRPLLELVAALVALAAAFVGGRWLNVLGSGELRSATAGLAAGSARISIFCAGAALTSI
                     AVIGGGMIGFVGLIAPHMVRLSFKSSDHRVIAPAAALAGGTLLCLADIIGRTVAEPRE
                     LPVGAVMALIGAPVFIVLLRRQTR"
BASE COUNT         1198 a         2030 c         2120 g         1178 t
ORIGIN      
        1 gaccggtggt gccgtcgtcc ccggtgcggg tgacgatctt cgatagacga ttccccatgc
       61 agctacttta ctacagcgcg ccggacttcc ggcgcgagtt aaccgtcgtt atactcggcc
      121 ccgtttgagc acagggttcc cgcaagggat ttaagggaac gcggtaaatg ccgcggctgc
      181 ccccgcaact gtaaccggcg agcgatcgtc ttgaatgcca ctgaggcgtc atgcctcggg
      241 aaggtggacg aaagccagga cccggaagtc aggagacctg ccttgtgctg tcgtcctgtt
      301 tccggtcggg gtgtgccggg atcgcggtaa cccgttgcga cgacacggtg gagtcgtcgc
      361 gggggttctt catctcaagg ctttcgcgtg ccgactcctg gaatgaaaac caggaggatc
      421 aatgcgcatt caatggttag gttcaatcgt aggggtgctg gcagccagtg ccagcggtgt
      481 cgcgcgtgcg gatagcccgg acttcgcaga cacactgcag ccggtggtgg tcacagcaac
      541 caagataccg acgcccgaat cacagctcgc cagcagcgta acggtcatta gcgcggagca
      601 gatcgccgcc atgcaggcga cgacgctccc ggacgtgctg aaatacgtgc cagggctgaa
      661 tctggtgcag tcgggcggtc caggcgggca gacctcggtg ttcatgcgcg ggaccaactc
      721 caatcacgtc aaggtgacgg tggatggcat cgacgtcagc gatccaggca cgccgaacgg
      781 ctcgtttgat ttcggccagt tcctggtgca ggacatcgag cgcatcgaag tgctgcgcgg
      841 gccgcagagc ggcctgtacg gctcggatgc gatcggtggc gtcatcaacg tcatcaccaa
      901 gaatggttcg ggtccaccgc agctggtggc tgcgctcgag gctggcagct tccagacttt
      961 caatcaagcg gtggctgtca gcggctcgca ggacgcgttc cactatgcag tgagcctcgc
     1021 gcatgagcac gccggctcga ctcccgtgac accgctggac ctgctggcgc cgggcgaagg
     1081 acggcgcaac gattacgacg acaatcttac cgtgtccacc aagctcagct acgtggccaa
     1141 ttcggccctc gactggggtg tcgtcggccg ttacaccgac acccatctgc atttcaccgg
     1201 tgacaacttc aacctcgtgc cggctgtacc cgatgcgaat cagagcgaga cagataccgg
     1261 cagttactat gggcgagtgt tccagcacct ggcgctgctg gacggccggc tcgatcagac
     1321 gctggacgtc gcctacacgc gcaaccggag cgacaacttc tcactcgaca acggcgagtc
     1381 gctcgctatc ggtcagcgcc tgaagttcga ctaccagggc gcattcaaga tcatcgattc
     1441 gcagaccctg gtcttcggtg ccgagcacgc acgcgatgag atcagcgagc cgatcgatgc
     1501 cggcctgtcg atcgactccg gctacctgga gttgcagtcg caactcgcgc agcggctgtt
     1561 ctccgcgatc aacgttcgct acgacagcaa ctcgctgttc ggtcataagg tcaccttccg
     1621 catcgcgccc acctatgtga ttgccgagac gggcacgcag cttcgcgcca gcgtcggaac
     1681 cggcttcaaa gcaccgacgc tgtcgcagct gtttcagaac tttccgccga cgttctttgc
     1741 caatcccgac ctcaagcccg agagcagcat tggctgggat ctgggggccg agcaggcgtt
     1801 gctggccgga acggtgcgtg cgggagcgac ctatttccac atcaggttac gtgatctgat
     1861 cgattccaac gacaccttca ccaccaacat caatgtcgac aaggcgacga catcgggcgt
     1921 cgagagcttc atctcgtacc ggccgtgggc ggcgctgacc ttgcggctgg actacactta
     1981 taccgaggcg accgacgata cgacgcaaca ggagctgctg cgtcggccga agaacaagac
     2041 cagcctggac ggcaactggc aggcgactgc ggcgctctcc ttcaataccg atctgttgta
     2101 cgtgggccca tggaatgatt ttcctcgcta tggctttgca accgcgcagg cgccgggcta
     2161 tttcaccgtg aatgttgccg gcaactacaa gctgagatcg aacgtcgagg tctatgcgcg
     2221 catgaacaat ctgctggatc gtcgctatga ggatccgtcg gggttcctgc agccggcctt
     2281 cggcgtgttc gcaggcatca aggctcgcct gtgaaacgcc gcgcttgacg ccggagggcc
     2341 gcccgcgcgc aaactagcgg catgatgtcg aaggaactgc aggcggctct ggatgcggcg
     2401 cgcgcggcgg ccgaggtgat ccaggggatg tatcggcgca atttggcggt gacgctgaag
     2461 gctgataaat ccccggtcac cgaggccgat gtcaaagccg agcaggcgat ccgctcggtg
     2521 ctgcaggcgc gctttccgga ccatggtttc tacggcgagg agaccggtcg gcacggggtc
     2581 gacgctgaga atgtctggtt ggtcgatccg atcgacggca ccaagtcctt cgtccgcgac
     2641 tgccctttct tctcgacgca gattgcgcta ctgcgcgctg ggaggttggt gctgggtgtc
     2701 tcgtgcgcct cggcctatgg cgagctcgcg tgggccgaag agggcggcgg tgcgttcctt
     2761 gacggccaag ctatcagcgt cagcccggtg tccgaactgg gcagcgccat cctttccacc
     2821 ggcaatctca agacattgtc ggcgtccgct accggctggt tgcgattcgg agcgctggtc
     2881 gggcgcgtca atcggttgcg cggttacggt gatttcctgc actaccatct gttggcgcgc
     2941 ggcgcgctcg acgcggtgct cgaatcggac gtcaacatcc tggatatcgc ggcgctgacc
     3001 gtcatcgtgc gcgaagcggg tggccgcttt accgatctgt cgggcggcga agtgggtctg
     3061 cagaccacta ccgtgctggc gagcaatgcg ctgctgcacc cgcagctact ggaggcgttg
     3121 agctgatcag gcggcgatga atgcccggcg gggggctacg ccgatggcct cgatgggtgt
     3181 caggtagagg gctgagagtt cttccgccgt cagcacctgc gcagccggcc cgagttgata
     3241 gcgtccatcg ccatacagta gcagcacacg gtcggcgaat cgttcggcga gcgtcggatc
     3301 gtgaagggtt gcgatgacgc tggcgccctg actgcacaat tcgcggaaca cctgcatgat
     3361 gtggatctga tgatgcggat ccaggtgatt ggtaggttca tcgagcagat agatccgtgg
     3421 ctgctgcgtc agcaatgaag ccatggcagc gcgccgctgc tcgccgccgg aaagcgtgtg
     3481 cacgttgcgg cgtgtgaaat ctcccagtcc catacggcgc agtgccgcct gagcgatcgc
     3541 cagatcgtcg gccccctcgc gttgccagaa tttaagatga gggtgtcggc cgatgagcgc
     3601 cgcctccaat gcgctcagac tcagggcctc ttcgcgatcc tgcagcagca gacccagttg
     3661 ccgcgcaatc gttcggcgcg gcatggtcga gatatcctcg ccgccgagca gtaccttgcc
     3721 ttccgccacg cgattcaggc cagcgagcgc gtgcaggctc aggctcttgc cactgccgtt
     3781 gcgtccgagg atcgccagga actctgcgcg ccgcacggtc agcgtcaagt cctgaaccag
     3841 ggtgcgctca ccggcggtaa tggaaatccc ggtcgcctgt agcggatgag cagccaagtt
     3901 catgtgacgg ctctagggct ggagccgtat ggcctgccag gggccgccct gatagatacc
     3961 ctgctccaag ctcagcacgc gcgtccagcg cgcacgatcg tgcagacgtc cggcgccctc
     4021 gacccaaagt tcgcagccgt cgatccagag gtggaatccg ccccagtggg gcggacggtc
     4081 gatcgggacg ccaggatcgg gtgccgtgtc atcgctgctg atcggcgatg gcgtaccgaa
     4141 tcgctgtgcc atgcgctgtg ccgcgcgttc gagatcgctg cgcgagagca ggggcgcact
     4201 ttgctgactg gcccaggcgc cgatgcggct ctgccaggag cgtccggaaa aataggcatc
     4261 gctttcggcc gagctgctgc gtgaaacgat gccttccaga cgtacctggc ggtgcatcgc
     4321 atcccaatgc atcaccgccg cggcgcgagg attggctgac agttcgcacc ccttgcgcga
     4381 caggtagttg gtgtagaagg tgatgtagcc ggggtggggc acaatctgct tgcataacac
     4441 cacccgcgct gcagggctgc cctgactatt gaccgtggcg agcaccatgg cgttgggatt
     4501 gggctgtgcg cgccgggcgg tcgcctgctg cagccagtcg gccacgatcg acaggggttc
     4561 tgcgggcagc tgctcaggca aatgttcacg gtgaaacggc atcgcgacat ggtagctgca
     4621 gtggggcatc ggtgcatgct acaaagcttc cctttgccct tcaggtattg gtcgatatga
     4681 cactggatga gctgcgtcgg cacctcgacg aattggatga gcggctgctg cagatcattg
     4741 cgcagcgtca gagtaccagc cgcgagatcg cgcgcgtgaa acgctcgacc ggcgttccga
     4801 cgcgcgacta tggtcgcgag cgcgaggtga ttctgggcgc gcgtggccag gctatgaaga
     4861 tcggcgtatc gccggatctg gcggaaacat tcatgcggct gctgatccgc tcgtctctga
     4921 ccacacagga gcaggccagc gtcgtggcgc acggagccgg cagcggacag cgtgcgctgc
     4981 tgatcggtgg tgccggcaag atgggcgcct ggttcgcctc atttctgaca tcgcaggggt
     5041 tcgatgtgca gatcgcggat cctcgagcga tgtcgggtcc caacgtgatc gcggactggc
     5101 acgattcggc gctgacgcag gactacatcg tcgtcgcggc gccgctcggt gccaccaatc
     5161 agattctgca gcagctcgcg gagcgaaagc cgcgcggcgt ggtgctggat ctgggctcgc
     5221 tcaagtcgcc gctgcgtgca ggactcgagg cgctgcgcgc agccggcatc atggtcacgt
     5281 ccattcatcc catgttcggc ccggacactg aactactgtc gggccggcat gtgattttca
     5341 tcgatctggg caacgcagaa gcgctggcgc gtgcccgcac gctgtttgcg ccgacgatgg
     5401 cggagcaggt cgtgatggga ttggatgagc acgatcgact gatcgcctac gtgctggggc
     5461 tgtcgcacgc gctcaatatc gcgttcttca cggcactggc ggaaagtggc gaggccgcac
     5521 ccaggttggc gcggctgtcg agcacgacgt tcgacgcgca gttcgacatc gccagcaagg
     5581 tggccgagga gagtccggaa ctctatttcg aaatccagcg gctgaacgat ttcggcgccg
     5641 aatcgctcaa cgccctggca cgggcagtgg aggccgttcg cagcgctgta ttcgacaacg
     5701 accaggcccg attcacggca ctcatgcggc aaggcaatga atatgtcaaa gaccggcgcg
     5761 acagtgcggc ccgcagagtc tgagaatcac cgggcctagc gggtctgacg tctcagcaag
     5821 acgatgaaca ccggtgcgcc gatcagcgcc atgaccgcgc cgaccggcaa ttcgcgcggt
     5881 tccgcgaccg ttcggccgat gatgtcggcg agacacagca gcgtgccgcc ggccagggcc
     5941 gcagccggtg cgatcacgcg atggtcgctg ctcttgaagc tcagtcgcac catgtgaggt
     6001 gcgatcaggc ccacgaatcc gatcatgccg ccgccgatca ctgcgatcga cgtgagcgca
     6061 gcgccagcgc aaaaaatgct gatgcgcgcg ctgccggcgg ccagcccggc cgtggcactg
     6121 cgcagttcgc ctgatccgag cacattcagc cagcgtccgc cgacgaacgc cgccgccagt
     6181 gcgaccaggg ccgcgaccag ctccagcaac ggccgctgag cccagcccaa atcgcctgcg
     6241 agccagaaca gcattccgcg caactggccg ttatcagcga gtgtcaggag cactgtaatc
     6301 agagcgccgc aggcactcgc cagcaccaca ccacacagca gcaggcgcgt tgtgccgcca
     6361 ccgcgcgcca tcagcaggac gctcaggcca atcagcgtgc cgccggcggt cgcatagcca
     6421 tgcatgactg ccagatctgc tccagtgagc atcgcaagca gcgcgccgat cgccgcgccc
     6481 cccgagaccc cgagcaccag cggatccgcc aacggatttc gaaaca
//
LOCUS       DATMRM010000440         8029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3967036, whole genome shotgun sequence.
ACCESSION   DATMRM010000440 DATMRM010000000
VERSION     DATMRM010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8029
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3967036"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VNZ06_13355"
     CDS             <1..251
                     /locus_tag="VNZ06_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009618124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWW21781.1"
                     /translation="EREGLDRIFIEAGAEWRESGCSMCIAMNGDSVAPGEYAISTSNR
                     NFEGRQGPGARTLLASPLTAAASAVRGRVTDPREFLNG"
     gene            261..839
                     /gene="leuD"
                     /locus_tag="VNZ06_13360"
     CDS             261..839
                     /gene="leuD"
                     /locus_tag="VNZ06_13360"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWW21782.1"
                     /translation="MEPIRQIISRTVVLPYTNIDTDQIIPARFLRTTTREGLGKQLFS
                     DWRYEANGASKPEFPLNRPEASGCKVLVGGRNFGCGSSREHAPWALLDYGFRAVISSE
                     IADIFRTNSLKNGLLPLVVDEGTHQWLLANPGVEVSVDLSTSTLRLPTGIVANFPIER
                     FARHCLLNGVDELGFLLSKESETAGYEQRTNR"
     gene            836..1897
                     /gene="leuB"
                     /locus_tag="VNZ06_13365"
     CDS             836..1897
                     /gene="leuB"
                     /locus_tag="VNZ06_13365"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HWW21783.1"
                     /translation="MKARIAVIAGDGIGPDVTAEAVRALQSIAKRFGHEFSLSEAPFG
                     GVAIDRHGDPLPASTLQTCLSSEAVLLGAIGGPKWSSSNASVRPEAGLLRLRRELGVY
                     ANLRPVTVHPALRTISPLKAETVEDVDLLFVRELTGGIYFGAKSRDAHQASDLCVYTV
                     AEIERIVRVAARIALTRRRRLLSIDKANVLETSRLWREVCERVVRTEFPGLQLEHMLV
                     DSASMHLIRRPRDIDVAVTENMFGDILTDEASMLAGSLGLLPSASLGDGTRGLYEPIH
                     GSAPDIAGQGIANPYGAILSAALLLRHSLKLEEEARAVELAVHRSIDDGVRTPDIVAP
                     NHRAATTREAGDAVLAALG"
     gene            2018..3148
                     /gene="argE"
                     /locus_tag="VNZ06_13370"
     CDS             2018..3148
                     /gene="argE"
                     /locus_tag="VNZ06_13370"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF005710.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HWW21784.1"
                     /translation="MNTRELLDSLIAFPTVSRDSNLDLIEFVRMFLAARGITSELFID
                     ATGKKANLFATVGSKDRGGIMLSGHTDVVPVDGQDWSSDPFRMVERSGSLYGRGTADM
                     KGFIASALAAADRATRRTLRSPLHLALSYDEEIGCVGVRSLIDAMKQWHVRPRLCIVG
                     EPTLLRPAIAHKGNTALHAVCTGHAVHSALAPHGVNAIYLASDFIQRVRGLQRQIERS
                     GSRDPDFDVPYTTLHIGLIRGGLALNIVPDHCELVLEIRNMPGESPHALVESIRSEAA
                     EVGAPRQSIEITVTNEYPGLSTPADAEVVELASSATGERGRIKVGFGTEAGLFSAELR
                     IPCVVCGPGSISDAHRPNEFVSLDQLARCDAMLDSVLDTLSR"
     gene            complement(3145..3765)
                     /locus_tag="VNZ06_13375"
     CDS             complement(3145..3765)
                     /locus_tag="VNZ06_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWW21785.1"
                     /translation="MKRSSTEARASDRIRATAAELFYRQGIRAVGVDELVTRAKATKP
                     SLYRSFVSKDELIAAYLRDFAQAFWQRFDAAVDAHPGNPRAQLRELFARSARRTAHAH
                     YRGCGLTNAAIEYPDRAHPAHQVVQRTKREVRRRLRSIARRLGVREPHLLADGLTMLL
                     EGAYVSRQIFGTDSPGRSLDRLANQMIDRWPRRKRGKLGGRAPSRR"
     gene            3848..5149
                     /locus_tag="VNZ06_13380"
     CDS             3848..5149
                     /locus_tag="VNZ06_13380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW21786.1"
                     /translation="MTLALRRRLGQDYVFVVVAVTFLALLISAGLRATPSVLIQPLES
                     AFAWPRSLISLAAAIGIVLYGLVGPFAAAVMQQFGIRRTVLGAMALMAIATGTSAFMT
                     QPWHLVLLWGVLSGIGSGCVASVLGATIVNRWFVTRRGLVMGLMTASTATGNLVFLPI
                     LAAIAQRHGWRPVVVTVALATAFLVPVVHWLLPERPIDIGLRRFGALPTTAEESPAVG
                     GNPFSIALRALCGAMRRRDFWYLFTTFFICGFTTNGLVGTHLIALCGDHGIMEVRAAG
                     LLALMGVFDLFGTTASGWLTDRYDPRKLLFAYYSLRGAALVYLPYSNFSLTSLSVFAV
                     FYGLDWIATVPPTLRLTSDVFGDSAAPVIFGWILAGHQLGAGCAAFLAGYLRTVQGDY
                     VDAFVLAGLSGVIAAAIALLIRRSPTQRTLVGLAGQPSSAS"
     gene            complement(5093..5785)
                     /locus_tag="VNZ06_13385"
     CDS             complement(5093..5785)
                     /locus_tag="VNZ06_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21787.1"
                     /translation="MTARSFGKAVALDDLARTDHPHLTAADQCWCLARYVSGPGYRAG
                     QVNQLIANLKCAPSIAAADPARAGHKRRAIEEAAIAVRRAVSSGWAESVTWIPIPPSY
                     PPGDRNYDDRLMRVLRVAFASDDADVRSLLYQVRTEPADHLRVRRLSVDRLYESVRVN
                     VAALACRPVRASIVLFDDVLTTGKHYKCCERRLLQVLPHVPVSGLFLARRALSARGRR
                     LPRQPYQRALGR"
     gene            complement(5782..6738)
                     /gene="mobQ"
                     /locus_tag="VNZ06_13390"
     CDS             complement(5782..6738)
                     /gene="mobQ"
                     /locus_tag="VNZ06_13390"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HWW21788.1"
                     /translation="MYFQITPISRGRGRSAVAAAAYRAGERIRDERTGKLHNYARRDD
                     IPHKEILLPAGLDSERISWVKNRAQLWNAAERAERQRNSRIAREFQLGLPHELSPEQR
                     LNLARTFSQELCDRYRVVVDLAMHLPRPGSDPRNFHAHLMLTSREITANGFGGKAGLD
                     LQPGQMRARGLREGIAEIKLMRERWASLANEALRDANIDERVDHRTLAAQGIDRMPRA
                     RVPWSTVIRERRGLRSDIGERVRANHQARVQAKRQRQGERDPSAAKDPAKEATKDPSS
                     IKDVQRQAREAWMQMRRGASVDHDRSDDHSPRSPAPDDDFSL"
     gene            complement(6757..>8029)
                     /locus_tag="VNZ06_13395"
     CDS             complement(6757..>8029)
                     /locus_tag="VNZ06_13395"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HWW21789.1"
                     /translation="DRYRRGTMIAPGRAAHQRQRRVRISSDASRLRLAGVPIALLDET
                     KHFKLIGTTGTGKSTAIRQLLAQALARGDRAVVADPDSGYLASLAHRYRGDVILNPFD
                     AQSVQWDPFGELDAPYDADLLANALIPCCPDPAAQEWRSYARTLVSSLLRRCQSHHGS
                     SSELWRLLAVAGPEELRALLAGTPAQPFLEAENARMFGSIRAVGVSAMAALEHVRRQR
                     SQPFSVRRWIREGRGVLFVPYLASQISALRCVISAWMRLAIFEAMSQPEGQDLRLWFV
                     VDELDALGAIDGLKDALARLRKFGGRCVLGFQSLSQVSTTYGTGEAQTIIENCGNTLI
                     LRCSGSEQGGTSAFASRLIGEREVIRRQASHSRDREGLFGRQSMRRSLQISEQRLSES
                     AVLASELEQLPDLRGYLKTASSPVWRPVRMQ"
BASE COUNT         1420 a         2581 c         2481 g         1547 t
ORIGIN      
        1 cggaacgcga aggcctcgac cgaatcttca tcgaggccgg cgccgaatgg cgcgaatccg
       61 gatgctcgat gtgtatcgcg atgaacggcg actcggtggc tcccggcgag tacgcgatca
      121 gcaccagcaa ccgcaatttc gagggccgtc agggcccggg agcccggacg ctgctggcca
      181 gtccactcac cgcggcggcc agtgcagtcc gcggccgagt gactgatccg cgtgaattcc
      241 tgaacggttg agtgcgagcc atggaaccga ttcgacagat catttcgcgt acggtagtcc
      301 tgccctacac caatatcgac accgaccaga tcatcccggc gcgcttcctg cggactacta
      361 cgcgcgaagg cctcggtaaa cagctgttct cggattggcg ctatgaggca aacggcgcga
      421 gcaaacccga atttccgctc aatcgtcccg aggcgagcgg ctgcaaagtg ctggtcggag
      481 gtcgcaactt cggctgcgga tcctcgcgcg agcacgcgcc ctgggcattg ctggactatg
      541 gctttcgagc ggtcatcagc agcgagatcg ccgacatttt ccgcaccaac tctctcaaga
      601 atggtctgct gccgctcgta gtggacgaag gcacccacca atggctattg gccaatccgg
      661 gtgtagaggt gagcgtggat ctgagcacca gcaccctgcg gttgcccact gggatcgtgg
      721 cgaatttccc gattgagaga ttcgcacgac attgcctgct gaacggcgtg gacgaactgg
      781 gttttctgct ctccaaagaa tccgagaccg ccggttacga gcaaaggacg aatcggtgaa
      841 ggcgcgcatt gcggtcatcg ccggcgatgg catcggcccg gatgtgaccg ccgaagcggt
      901 gcgagcactg cagagcatcg cgaaacgctt cggtcacgaa ttttcgctat cggaagcgcc
      961 ttttggtggc gttgcgatcg atcggcacgg tgatccgctg cccgccagca cattgcagac
     1021 ctgcctttcc tctgaggcgg tcttgctcgg tgccattggc ggaccgaaat ggtcttcctc
     1081 caatgcgtcg gtgcgacccg aagccggcct gctgcgtctg cgcagggaac tgggcgtcta
     1141 tgcaaatcta cgaccggtca cggttcaccc agcgctacga acgatctcgc cgctcaaggc
     1201 ggagaccgtc gaggacgtgg acctgctgtt cgtgcgtgaa ctgactggcg gaatctattt
     1261 tggcgccaag agccgcgatg cccatcaagc cagcgacctt tgcgtctaca cggtagcgga
     1321 gatcgagcgc atcgttcgag tcgcagcgcg tatcgccctc actcgtcgtc ggcggctgct
     1381 gtcgatcgac aaggccaacg ttctagaaac ctcacgtctg tggcgcgagg tctgcgagcg
     1441 cgtggtgcgc actgaattcc ctggcctgca gctcgagcac atgttggtgg attctgcatc
     1501 gatgcacctg attcgccgtc cgcgcgacat cgatgtagca gttaccgaaa atatgttcgg
     1561 cgatattctc accgacgaag cgtccatgct ggccggatcg ctgggactgc tgccatcggc
     1621 atcgctcggt gatggaaccc gcggcctcta tgagccgata cacggttcgg cgccggacat
     1681 cgccggccag ggcatcgcca atccctacgg tgcgatcctc agcgccgccc tgctgctacg
     1741 ccattcactc aagctggaag aggaagcgcg cgccgtcgag ctcgctgtcc accgctcaat
     1801 cgacgacggc gtgcgcactc ctgacattgt cgcgcccaat catcgagcag ccaccacgcg
     1861 tgaagccggc gacgccgtac tggcggcatt aggctgagac gacgcgggac cggccccatt
     1921 tcatcgcgta gtagtacagc ggcgaggtca ccaccagccc gacgatccag gatacgttga
     1981 tcgtcgaaaa cagtgcgcgt acgagtaaga tcggaacgtg aacacgcgcg agctactgga
     2041 cagcctgatc gcttttccca ctgtcagccg ggactccaac ctggatctga tcgagttcgt
     2101 gcgcatgttc ctggccgcgc gtggaatcac cagcgagttg ttcatcgacg ccactggcaa
     2161 aaaggcgaac ctttttgcaa cagtcggctc gaaggatcgc ggcggcatca tgctgtcggg
     2221 tcacaccgac gtcgtgccag tggacggcca ggactggagc agcgaccctt tccgcatggt
     2281 ggagcgcagc ggctcgctgt acggccgcgg aaccgccgat atgaagggtt tcattgccag
     2341 cgccctggcc gctgccgatc gagccacccg acgcacgctg cgctcaccgc tgcacctggc
     2401 tctctcctac gatgaggaga tcggctgcgt tggcgtgcgc tccctgatcg acgccatgaa
     2461 gcaatggcat gtgcgtccca gactgtgcat cgtcggggag ccgaccttgc tgcgcccggc
     2521 gatcgcacac aagggcaata ccgccctgca tgctgtctgt accggacatg ccgttcactc
     2581 ggcacttgca ccgcacggcg tgaatgccat ttacctcgcg agtgatttca tccagcgtgt
     2641 ccgggggctg cagcgacaga tcgagcgctc cggaagtcgc gaccccgact tcgatgtgcc
     2701 ctacaccacg ttgcacatcg gcctcattcg cggtggcctg gcactgaata tcgtcccgga
     2761 tcattgcgag ctggtactgg aaatcaggaa catgccgggt gagtcgccgc acgcgctggt
     2821 cgagtcgatc cgaagcgaag ctgcggaagt tggcgcgccg cggcagtcca tcgaaatcac
     2881 tgttacgaac gagtaccccg gcctgtcaac gccggcggat gccgaagtcg tcgaactggc
     2941 cagttccgcc accggcgagc gcggccgcat caaagtgggt ttcggcaccg aggccggcct
     3001 cttcagcgcc gagctccgca tcccttgtgt ggtgtgcggt cctggctcga tcagcgacgc
     3061 tcatcggccg aacgagttcg tcagtctgga tcagctcgcg cgctgcgatg ccatgctcga
     3121 ttccgtgctg gatacgctta gccgctagcg ccgcgacggt gcgcggccgc cgagcttgcc
     3181 acgcttgcgg cgcggccagc ggtcgatcat ctgattggcg agtcggtcga gtgagcgccc
     3241 cggactgtcg gttccaaaaa tctgacgact gacataggca ccctcgagca acatggtcag
     3301 cccatccgcc agcaggtggg gctcacggac tccaagccgc cgcgcgatcg agcgcagacg
     3361 acgacgcacc tcgcgtttgg tccgctgcac gacctggtgt gccggatgcg cccggtccgg
     3421 atattcgatc gctgcgttcg tgaggccgca gccccggtaa tgcgcgtgag cggttcgcct
     3481 ggccgagcgc gcaaacagtt cgcgcagctg cgctcgcgga ttccccgggt gcgcatccac
     3541 tgccgcgtca aacctctgcc agaacgcttg cgcaaagtca cgcaggtacg cggcaatgag
     3601 ctcatccttg gaaacgaagc tgcgatacag gctgggcttc gtcgccttgg cgcgggtaac
     3661 caattcgtcc accccgacag cgcggatgcc ctgccggtag aacaattcgg cggcagtggc
     3721 gcgaatccga tcactggcgc gggcttcggt gctggagcgc ttcatttttt tatagcgatg
     3781 atgacttgac ttcgttaccg atcggtacgt agcattatct gccccaagat acgtaccggt
     3841 cagtaacatg actctagcat tgcgccgccg gcttggccag gactacgtct tcgtggtcgt
     3901 cgcggtgacc ttccttgcgc tgctgatcag cgccggtctt cgagcgacgc cgagcgtttt
     3961 gattcagccg ttggaatctg cgtttgcctg gccacgctct ctcatatcgc tggcagccgc
     4021 gatcggcatc gttctgtacg ggctggtcgg accgttcgca gccgcggtga tgcagcagtt
     4081 cgggattcga cgcaccgtgc tcggtgcgat ggctctgatg gccattgcga ccggcaccag
     4141 cgccttcatg actcaaccct ggcacttggt gctgctgtgg ggcgtgctgt cagggatagg
     4201 cagcggttgc gtggcatcgg tgctcggcgc gacgattgtc aatcgttggt tcgtgactcg
     4261 ccgcggcctg gtcatgggat tgatgaccgc gagcaccgcg accggcaatc tggttttcct
     4321 tcccattctg gcagccatcg cgcagcgtca tggatggcgg ccagtggttg tcacggtcgc
     4381 gctggcgacg gctttcctgg ttcccgtcgt tcactggctg ctgcctgaga ggccgatcga
     4441 tatcggattg cggcgctttg gcgccctacc gaccacggcc gaggagtcgc ccgctgttgg
     4501 cggtaacccg ttcagcattg cgttgcgagc gctctgcggc gcgatgcgcc gacgcgactt
     4561 ctggtacctg ttcacgacct ttttcatttg cggctttacg accaacggcc tggtaggtac
     4621 acatctgatc gcgctgtgtg gcgaccatgg gatcatggaa gttcgggccg cgggactgct
     4681 ggcgctgatg ggagtgttcg acctattcgg cacgactgcg tccggctggc tcacggaccg
     4741 ctacgatccg cgcaaactgc tgtttgccta ttacagcctg cgcggcgcgg cgctcgtcta
     4801 cttgccctac tccaatttct cgttgacgag cctgtccgtg ttcgccgtgt tctacggtct
     4861 ggactggatt gccaccgtcc cgcccacgct gcgcctcacg tctgacgtat tcggagatag
     4921 cgcagccccg gtcattttcg gctggatact ggccggtcat caattaggag ccgggtgcgc
     4981 tgcctttctt gccggatatc tgcggaccgt ccagggcgac tatgtggacg ctttcgtcct
     5041 cgcgggatta tcgggagtca ttgccgcagc catcgcattg ctcatcagac gatcaccgac
     5101 ccagcgcacg ctggtagggt tggcggggca gccgtcgtcc gcgagctgac agggcgcgtc
     5161 gcgccaggaa caatccgctg actggcacgt gtggtagtac ctggagcagg cggcgctcgc
     5221 agcatttgta gtgtttgcca gtggtcaaga catcgtcgaa cagcacgatc gaggctcgca
     5281 ccggccgaca tgcgagcgcg gccacattga cgcggacgga ctcatacagg cggtccacgc
     5341 tcaggcgccg cacccgcaag tggtccgcgg gttcggttcg cacctggtag agcaggctgc
     5401 gcacgtcggc gtcatcgctg gcgaacgcta cgcgcagaac acgcatgagg cggtcatcgt
     5461 aattccgatc ccctggcggg taggatggcg gtatcggaat ccacgtgacg ctttcagccc
     5521 agccgctgct gacggcgcgg cgcaccgcga tcgccgcctc ctcgatggcg cggcgcttgt
     5581 gaccggcccg tgccgggtcg gccgcggcga tagagggtgc acacttgaga ttggcgatca
     5641 gctggttgac ctgacccgcc cggtagcccg ggcccgacac atagcgcgcc agacaccagc
     5701 actgatcggc ggcagtcaga tgcgggtgat cggttcgagc cagatcgtcc agggccaccg
     5761 ccttaccgaa gctgcgcgca gtcatagcga gaagtcgtcg tccggagcag gagaccgcgg
     5821 cgaatggtcg tcggagcggt catgatcaac cgacgctccg cgtcgcatct gcatccaggc
     5881 ctcacgcgcc tgccgctgca cgtctttgat gctcgatgga tccttggtag cttcctttgc
     5941 gggatccttt gccgctgacg ggtcccgttc accctgtcgt tgtcgctttg cctgcacccg
     6001 cgcctgatga ttggcgcgga cgcgctcacc gatatcgctg cgcaggcccc tgcgctcacg
     6061 tatcaccgta ctccagggga ctcgtgccct gggcatgcga tcgataccct gagccgccag
     6121 cgtgcgatga tcgactcgct catcgatatt cgcgtcccgc aacgcctcgt tagccaagct
     6181 ggcccagcgc tcgcgcatga gtttaatctc cgcaatgcct tcgcgcagtc ctcgcgctcg
     6241 catctgtcct ggctgcagat caagtccagc tttgccgcca aatccgttgg cggtgatctc
     6301 acgcgacgtg agcatcaggt gggcatggaa atttcgtgga tcgctgccgg ggcgcggtag
     6361 gtgcatcgca agatccacca cgacgcgata gcgatcgcag agctcctgcg aaaaagtgcg
     6421 cgcgagattc aatcgttgct cgggcgaaag ctcgtgcgga agaccgagct ggaactccct
     6481 ggcgatgcgc gaattgcgct gtcgttcggc acgttcggcc gcattccaca actgagcgcg
     6541 attcttgacc cacgaaatgc gctcgctgtc gagccccgca ggcagcagaa tctccttgtg
     6601 cggtatgtcg tcgcggcgag cgtagttgtg aagtttcccg gtgcgctcgt cacggatccg
     6661 ttcaccggcg cggtacgcag ccgccgccac ggcactgcgt ccccggccac gcgaaatcgg
     6721 cgtgatctga aagtacatga tcgccacagg acggtcctat tgcatccgta cggggcgcca
     6781 gacgggggaa gatgcggtct tcagataccc ccgaagatcc ggcaactgtt ccagctcgga
     6841 ggcaagcacc gcactttccg agagccgctg ctcgctgatc tgtagcgacc ggcgcatcga
     6901 ttgccggccg aaaagccctt cccggtcgcg gctgtgtgag gcctgcctcc tgatcacttc
     6961 gcgctcgcca atcaggcgcg acgcgaacgc tgaggtcccg ccctgctcgc tgcccgagca
     7021 gcgcaggatc aacgtgttgc cgcagttttc gatgattgtc tgtgcctccc cggtgccgta
     7081 ggtcgtagac acctgcgaaa gcgattgaaa tcccagtacg cagcgtccgc cgaacttgcg
     7141 cagtcgggcc agtgcatcct taagaccgtc aatcgcaccc agggcatcca actcgtccac
     7201 cacgaaccac agtcgcaggt cttgtccctc cggttgactc atcgcctcaa agattgccag
     7261 tcgcatccac gcggagatca cacaacgcaa tgcggatatc tgcgaagcca ggtaagggac
     7321 aaacaacact ccccgccctt ctcgaatcca gcgtcgcacc gaaaacggct gggatcgctg
     7381 ccgacgcacg tgctccagag ctgccatggc cgacacgccc actgcgcgaa tcgatccgaa
     7441 catgcgtgcg ttctccgcct ccaggaatgg ttgcgccggc gttccggcga gcagagctcg
     7501 cagctcctca ggtcccgcga ccgccagtag ccgccacagc tcactactgc tgccgtgatg
     7561 ggattgacag cgccgcagga gcgaggagac gagcgtgcgg gcgtagctgc gccactcctg
     7621 cgccgccgga tccggacaac agggtatcaa tgcgttggcc agcagatcgg cgtcgtaggg
     7681 cgcatcaagc tccccgaagg gatcccactg caccgattgc gcatcgaacg gattgagtat
     7741 cacgtctccg cgatagcgat gcgcgaggct ggccaaatac ccgctgtccg gatcggccac
     7801 cacagcccga tcgcctctgg ccagggcctg cgccaacaat tgccgaatcg cggtcgactt
     7861 gccggtgccg gtagtaccga tcagcttgaa atgtttggtt tcatcgagca gcgcaatcgg
     7921 cactccggcc aaccgcaatc gcgaggcatc gctcgagatg cgcacccggc gctgcctctg
     7981 atgcgctgca cggcccggcg caatcatcgt accgcgccgg tagcgatcg
//
LOCUS       DATMRM010000441         6229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1190864, whole genome shotgun sequence.
ACCESSION   DATMRM010000441 DATMRM010000000
VERSION     DATMRM010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190864"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VNZ06_13400"
     CDS             complement(<1..629)
                     /locus_tag="VNZ06_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HWW21790.1"
                     /translation="MTRLWDKGTSLDQRVLQYTAGEDYALDERLVSYDVRASIAHAQM
                     LHSQKLLSSTDLEQIVNGLTALGEEHQRGLWRIELADEDVHTALERHLTARIGEAGGR
                     VHLGRSRNDQVLTALRLYLRDAIEQLDAGAHRLIGALEQLAAREASTSLPGYTHMQQA
                     MPSSVALWAGGYAAELLDDARGIQSVHRRIEKNPLGSAAGYGTPGLPIDR"
     gene            complement(626..1561)
                     /gene="argB"
                     /locus_tag="VNZ06_13405"
     CDS             complement(626..1561)
                     /gene="argB"
                     /locus_tag="VNZ06_13405"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWW21791.1"
                     /translation="MILSRSDHSATIRALRNASPYIRLYKGKIFVVKAGGAVFGDPAL
                     TRALVEQISILHHLGVRVVMVHGGGPQLTAVSEALGVPTRMVQGRRITDERSIDATAM
                     VLNGLINTRILAICRDLDIAAVGLSGVDAGLVRAHRRPPMRLHGEVDMVDFGFVGDID
                     MIDASVIRKHLDNGLMPVISPLSADDQGVLLNINADTVAAALGAALGAEKLILCTGAP
                     GILERVEDLTSVVSYTDLAGLKRLREEGRIVDGMLPKAKAIEDAIRGGVRRVHVISYS
                     AQDSILAEVFTNEGTGTLIVADTNALTPAEQSAHK"
     gene            complement(1572..2576)
                     /locus_tag="VNZ06_13410"
     CDS             complement(1572..2576)
                     /locus_tag="VNZ06_13410"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HWW21792.1"
                     /translation="MRRFVDLADFSRDEVLTLLELARRLETRPEPHALSGKMLGLLFM
                     NPSLRTLASFQSGMARLGGASFVVTPGAGTWQLETRPGVVMDGAAAEHVTEGLPVLAS
                     YCDALGIRSFADGKDLAADVSEQKFNAMSRLIDKPLINLESAVNHPCQSLADWKTLDE
                     IGVPRNGKFVLSWAYHPRPLPLAVPAATLHMAAMRGMEVVVLRPNGFALPDALMEKAR
                     RAAGAAGGSVRETSDRREALEGTHIVYVKEWALTANYGDEAADAAARRQLKDWCCVES
                     WFSTARPDARLMHCLPVRRNVAVADAVLDSSRSVVLRQAYNRLPAQMAVLYRLLKQAE
                     "
     gene            complement(2586..3755)
                     /locus_tag="VNZ06_13415"
     CDS             complement(2586..3755)
                     /locus_tag="VNZ06_13415"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWW21793.1"
                     /translation="MNAQPQSQSGALHDYLAPVFAQYPLPVASGEGVWLNTTDGRRVL
                     DLYGGHAVAALGYGHPAWTRALTEQARTLSFQTNAVPIDVRVRAARKLIHFSQLDFNS
                     VFFVNSGAEANENALKMAFRITGRSHVAALEQSFHGRTAGAGAVTWGSAAKWYGFPRT
                     PFDVSFLPRRDLPAIAQQVTDRTAAVIIEPVQGQAGAFDHGAAYLNALRQRCTEVGAL
                     LIFDEVQCGMGRVGEPFAARLYGVQPDMITTAKALGSGFPCSALLMSPRVASAVKLDS
                     LGTTFGGGPMACALIEAVIDTIESERLLERVRATGALIRSRCIVGPITGHQGAGLLVG
                     LRTGRPAKEVHAALLERGILAGTAADPNILRLIPPFVLEAGHVELLRAALLDVGA"
     gene            complement(3752..4813)
                     /gene="argC"
                     /locus_tag="VNZ06_13420"
     CDS             complement(3752..4813)
                     /gene="argC"
                     /locus_tag="VNZ06_13420"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HWW21794.1"
                     /translation="MGPSIPTVVLGGSGYVAGELLRLIAGHPHFSLAGVLSDSQPGER
                     VAQAFGHLAPVLPEERFRPLSEIEAIVAREDSTAIFSAAPHGAAAALIDSLLCKAEAA
                     QRKPHVVDISADYRYRSADAYQAVYKHPHGAPQRICAFSCAVPEHLPELTTAHVAHPG
                     CYATAALLASVPLLALSLSTPELFVSAVTGSTGSGRKPGEGTHHPLRHGDLYSYGGLT
                     HRHTPEITACARAASGVDAQFAFIPHSGPFARGIHATVQARLRQPLDTAQLLAALRRF
                     YQGKPFVRVSDTMPRVKEVAGSNYAQLSGVSDGRTVAVMCALDNLNKGAAGGAVQWMN
                     RLYGLAETAGLTAPAAGWT"
     gene            complement(4815..5894)
                     /locus_tag="VNZ06_13425"
     CDS             complement(4815..5894)
                     /locus_tag="VNZ06_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21795.1"
                     /translation="MKTVIVDKIASVTQALELNHELRLSSDIPAEEGVVVVAEILNNK
                     ANYNTLELTSGRMAKVAKGDIVVGALGPRRALFGYSGHVPTSVKPGDIIQMLNIGGVM
                     GICDSATPDKGKPFDCRVLGVVLQFPYLGERIGVPARVGMQQLDFNAHLDTGRVPVVA
                     LAGTCMEAGKTAAAAAIVGRMRHRGLVVDAFKATGVSLRRDILAFSDAGARRTMIFTD
                     LGVVTTTPRTGPALTRTMLTELAAGKPDAIVFELGDGLLGTYGVDAILECADIRKSLT
                     AVVLSANDPVAAWGGVKLLRERFHVDPCAVTGPSTDNQVGVEIIEQQMKVAAYNALSN
                     AAALGDFVIESLGIASQARAAAGAE"
     gene            complement(5891..>6229)
                     /locus_tag="VNZ06_13430"
     CDS             complement(5891..>6229)
                     /locus_tag="VNZ06_13430"
                     /inference="COORDINATES: protein motif:HMM:NF044952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21796.1"
                     /translation="RQQRIKESVAQPYGDLVHEGQLLDPVCRDIEALMVSSQERVTGE
                     VHVLFRPGNLWVEGVDSPHSLMAASKGVYGEAAGEWTAIDALGFSKIVALSGVFYRRA
                     GDSAAQGSKP"
BASE COUNT         1123 a         2065 c         1967 g         1074 t
ORIGIN      
        1 cgatcaatcg gcagaccggg ggtgccatag ccagcagccg atccgagcgg attcttctcg
       61 atgcgccgat ggacgctctg gattccgcgt gcgtcgtcca acaactcggc ggcatagccc
      121 cctgcccaca gtgcaaccga actcggcatc gcctgttgca tgtgggtata gcccggcaat
      181 gaagtggagg cttcgcgcgc cgcaagttgc tcgagtgcgc cgatgaggcg gtgcgcgcca
      241 gcgtcgagtt gctcgatggc atcacgcagg tacagccgca gagccgtcaa cacctgatcg
      301 ttgcgcgagc ggcccagatg tacccggccg ccggcctcac cgatgcgcgc cgtcagatgt
      361 cgctccagcg ccgtgtgcac gtcttcgtcg gccagctcga tgcgccacag ccctcgttga
      421 tgctcttcgc ccagtgccgt caggccgttc acgatctgct cgagatcggt gctcgacagc
      481 agcttctgcg aatgcagcat ctgcgcgtgg gcgatggatg cgcgcacgtc gtagctgacc
      541 aggcgctcgt cgagtgcata gtcctcgccg gcggtgtatt gcaatacccg ttgatcgagc
      601 gacgtaccct tgtcccacag ccgcgtcatt tgtgcgctga ctgctccgca ggcgtgagtg
      661 cgttggtatc cgcgacgatc agggtgccgg tgccctcatt ggtgaacacc tcggccagaa
      721 tgctgtcctg tgcgctatat gaaatgacat gcacgcggcg cacaccgcct cgaatcgcat
      781 cttcgatcgc tttggctttc ggcagcatgc catcgacgat ccgcccctcc tcgcgcaagc
      841 gcttcagccc ggccaggtcg gtgtaggaga cgaccgaagt gagatcttca acgcgctcca
      901 ggattcccgg tgccccggtg cacagaatca gcttctcggc tcccaatgcg gcaccgagcg
      961 ccgcggccac ggtatcggca ttgatgttga gcaacactcc ctgatcgtcg gccgacagcg
     1021 ggctgatgac tggcatcagg ccgttgtcca gatgcttgcg gatcacactg gcgtcgatca
     1081 tgtcgatgtc accgacaaat ccgaagtcca ccatgtcgac ttcaccgtgc aggcgcatcg
     1141 gaggccgccg atgtgcccgg accagacccg cgtccacacc cgataaaccc acggccgcga
     1201 tatccagatc acgacagatc gccaggatgc gggtgttgat caatccgttc agcaccatgg
     1261 cggtcgcatc gatcgagcgc tcgtcggtga tccgccgacc ctgcaccata cgtgtcggca
     1321 ccccgagagc ttcactcact gcggtgagct gcggtccgcc gccatgcacc atcacgacgc
     1381 gcacgcccag gtgatgcagg atcgagatct gttcgaccag cgcgcgagtg agcgccgggt
     1441 cgccgaagac agcgccgccg gccttcacga caaagatctt gcccttgtaa aggcggatgt
     1501 agggcgacgc gttgcgaagc gcccggatgg ttgcggaatg atcggatcga gagaggatca
     1561 tacggtcgtg gttactccgc ttgcttgagc aatcgataca gcactgccat ctgcgccggc
     1621 agacggttgt aggcctgccg cagcacgacg ctgcgcgagc tgtcgagcac ggcatcggcg
     1681 accgcgacat tacgccgcac cggcagacag tgcatcagcc gcgcgtccgg acgcgcagtg
     1741 gaaaaccagg attcgacgca gcaccagtcc ttgagctggc ggcgcgctgc ggcgtcggcg
     1801 gcttcgtcgc cgtaattcgc agtcagcgcc cactccttca catacacgat gtgcgttcct
     1861 tcgagcgctt cgcggcgatc gctggtttca cgcaccgagc cgccggccgc gcccgctgcg
     1921 cggcgcgcct tttccatcag cgcgtcgggc agtgcgaagc cattcgggcg tagcaccacc
     1981 acttccatgc cgcgcatcgc ggccatgtga agcgttgcgg ctggcaccgc gagcggcagc
     2041 ggtcgcggat gataggccca gctaagcacg aatttgccat tgcgcggcac tccgatttca
     2101 tccagggtct tccagtccgc caacgactgg caggggtgat tcaccgccga ttccagattg
     2161 atgagcggct tgtcgatcag tcggctcatc gcattgaatt tctgttcgct gacgtccgcg
     2221 gccagatcct tgccatccgc aaacgaacgg ataccgagcg catcgcagta ggaggcgagc
     2281 accggcaagc cttcggtgac atgttccgca gcggctccgt ccatcacaac gcccggccgg
     2341 gtttcaagct gccaggttcc ggcaccgggt gtaacgacga aagaggcccc gcccaaacgc
     2401 gccatgccgc tctgaaacga cgccagcgtg cgcaacgaag gattcatgaa caagagcccc
     2461 agcatcttgc cgctcaacgc atgcggttcg ggccgcgttt ccaaccggcg cgcgagctcc
     2521 agcagcgtca gtacttcatc acgcgagaaa tcagccaggt ccacgaatcg acgcacgatg
     2581 gacctctagg cgccgacgtc gagcagcgcg gcgcgcagca gttcgacgtg gccggcttcc
     2641 agcacgaacg gcgggatcag gcgcaggatg tttgggtcgg ccgctgtgcc tgccagaatt
     2701 ccacgctcga gcaacgccgc gtgaacctcc ttggccggtc gtccggtgcg caatccgacc
     2761 agcaggccgg cgccctgatg gccggtaatc gggccgacga tgcagcggct gcggatcagc
     2821 gctccggtgg cacgcactcg ttccagcaac cgctcggatt cgatcgtatc gatgaccgcc
     2881 tcgatcagcg cacaggccat cgggccgccg ccgaatgtcg tgccgagcga atccagcttc
     2941 acggcgctgg cgacgcgcgg tgacatcagc aatgccgagc acggaaagcc gctgcccagc
     3001 gccttcgccg tcgtgatcat gtccggctgc acgccataga gccgggcagc aaacggttcg
     3061 ccaacgcgtc ccatgccaca ttgcacttca tcgaagatca gcagcgcgcc gacttcggtg
     3121 cagcgctggc gcagcgcgtt cagataagca gcgccgtgat cgaatgcacc ggcctgaccc
     3181 tgcaccggct cgatgatcac cgcggcggtg cgatcggtca cctgctgcgc gatcgccggc
     3241 aggtcgcgtc gcggcagaaa gctgacgtcg aacggggtgc gcggaaagcc gtaccacttg
     3301 gccgccgatc cccaggtcac ggcaccggcg ccagcggtac ggccgtgaaa cgactgctcg
     3361 agcgcggcca catgcgagcg gccggtgatc cggaacgcca tcttgagcgc gttctcgttc
     3421 gcctccgcac cgctgttgac gaagaacaca ctgttgaaat cgagctgcga gaagtgaatc
     3481 agcttgcgcg cggcacgcac gcgcacgtcg atcggcaccg cgttggtctg aaagctcagg
     3541 gtgcgcgcct gctcggtgag cgcccgcgtc caggccggat gcccgtagcc cagagccgcg
     3601 accgcgtgac cgccatatag atcgagcacg cgacggccat ccgtcgtgtt cagccacacg
     3661 ccctcgccgg acgcaaccgg caaagggtac tgtgcgaaca ccggcgccag atagtcatgc
     3721 aatgcgccgg actgtgattg cggttgtgcg ttcatgtcca tcctgccgct ggtgcggtga
     3781 gtccggcggt ttctgcaaga ccatagagac gattcatcca ctgcaccgca ccgcccgcgg
     3841 cgcctttgtt gagattgtcg agcgcgcaca tcacggccac cgtgcgaccg tcactaacgc
     3901 ctgacagctg cgcgtagttg ctgccagcca cttccttgac gcgcggcatg gtatcgctga
     3961 cgcgcacgaa gggcttgccc tgatagaacc ggcgcagcgc ggcgagcagc tgcgcggtgt
     4021 cgagtggctg ccgcagccgt gcctgcactg ttgcgtggat gccgcgcgca aaaggtccgg
     4081 agtgcgggat gaaggcgaac tgcgcatcga caccactggc tgcgcgtgcg cacgcagtga
     4141 tttccggtgt gtgccggtgc gtgagtccgc catagctgta gagatcacca tgccgcagcg
     4201 gatgatgcgt gccctcgccc ggcttgcggc cggaaccggt gctgccagtg accgcggaga
     4261 cgaacaattc cggcgtgctg agcgacagcg ccagtagcgg caccgatgcg agcagcgccg
     4321 cggttgcgta gcatcccgga tgcgcgacat gcgcggtagt cagttcgggc aagtgttccg
     4381 gcacggcaca gctgaacgcg cagatgcgct gtggtgcgcc gtgaggatgc ttgtagactg
     4441 cctggtaggc atcggcagag cggtagcggt agtccgcgga aatgtccacc acgtggggct
     4501 tgcgctgcgc ggcttcggct ttgcagagca gcgaatcgat cagtgccgcg gcggcgccat
     4561 gcggagccgc tgagaagatc gccgttgagt cctcgcgcgc gacgatcgct tcgatctcgc
     4621 tcaaaggtct gaatcgctcc tcgggaagca ccggtgccag atgaccaaac gcctgcgcga
     4681 cacgctcacc cggttggctg tctgacagca cgcccgcgag cgagaagtgt ggatgaccgg
     4741 cgatcagtcg cagcagctca ccggcgacat agccgctgcc acccagcacg accgtgggaa
     4801 tgctcgggcc catgctactc cgccccggcg gcggcgcgtg cctgagaggc gatccccagc
     4861 gactcgatca caaagtcacc cagcgcggcg gcattgctca gcgcgttgta cgccgccacc
     4921 ttcatctgct gttcaatgat ttcgactccg acctggttgt cggtcgacgg accggtcact
     4981 gcgcacgggt cgacgtgaaa gcgctcgcgc agcagcttga caccgcccca ggctgcgacc
     5041 gggtcgtttg ccgacagcac gacggcagtc agagacttgc gaatgtcggc gcattccaga
     5101 atcgcgtcga cgccataggt gcccagcagc ccgtcgccca gctcgaacac aatggcgtcc
     5161 ggcttgccgg cggccagttc cgtgagcatc gtgcgcgtga gtgcagggcc ggtacgaggc
     5221 gtcgtcgtga ccacgccgag atccgtgaag atcatcgtgc gtcgcgcgcc agcgtccgaa
     5281 aaggcgagga tatcgcgtcg cagcgagacg ccggtggcct tgaaagcgtc gaccacaagt
     5341 ccgcgatgac gcatgcgtcc gacaatggcc gccgccgcgg cggtcttgcc ggcttccatg
     5401 caggtgccgg cgagtgcaac caccggcaca cggccggtat ccagatgggc gttgaagtcc
     5461 agttgctgca ttccgacgcg cgctggcacg ccgatgcgtt ccccgaggta gggaaactgc
     5521 agcaccacac ccagtacgcg gcagtcgaac ggcttgccct tgtcgggcgt ggccgaatcg
     5581 cagatgccca tgacgccgcc gatgttgagc atctgaatga tgtcgcccgg tttgaccgat
     5641 gtcggcacat gacctgaata gccgaacagc gcacgccgtg gaccgagcgc gccgaccacg
     5701 atatcgccct tggccacctt ggccatacgt ccgctggtga gttcgagcgt gttgtagttg
     5761 gccttgttgt tgagaatctc ggccaccacg acgacacctt cctccgccgg aatgtcgctg
     5821 gataggcgca actcgtgatt gagctcgagc gcctgcgtga cggaagcgat cttgtcgacg
     5881 atgacggttt tcatggcttg ctaccttgcg cggcgctgtc accggctcga cggtaaaaca
     5941 cgccgctgag cgcgacgatc ttggagaacc ccagtgcatc gatggcggtc cattcgcccg
     6001 cggcctcacc gtacacaccc ttcgatgccg ccatcagtga atgcggcgag tcaactcctt
     6061 cgacccacag attgcccggg cggaacagga catgcacttc gcccgtgacg cgctcctgcg
     6121 agctgaccat gagcgcctcg atgtcgcggc acaccggatc gagcagctgc ccctcgtgca
     6181 ccaggtcgcc atagggctgc gccaccgatt ccttgatgcg ctgctggcg
//
LOCUS       DATMRM010000442         4778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3338676, whole genome shotgun sequence.
ACCESSION   DATMRM010000442 DATMRM010000000
VERSION     DATMRM010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3338676"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VNZ06_13435"
     CDS             complement(<1..868)
                     /locus_tag="VNZ06_13435"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HWW21797.1"
                     /translation="MMRLLFVDDDPVALEQIRREISHARLHYDVVFVDSGRGALAALE
                     GGEFDAIASDLRMPDMDGPTLLAQVRNQFPNVVRVCMSAGMEDADFMRAMPVTHQFLS
                     KPIVGDALREVIERACALNSILHHQGIRALVGRLNALPATAQTFQDLSDAMTEPNTHT
                     ADISRIVSKDTALCVKTLQIVNSSFFRRRQAITSIPAAVTFVGMEMIKSLALSACVFN
                     ALDASPAALKLMRDLQARSLRKAHFARSLLKDSRHADEAFTAALLLDIGQAVLAMCSP
                     ETFAEVIQIARES"
     gene            complement(865..2844)
                     /locus_tag="VNZ06_13440"
     CDS             complement(865..2844)
                     /locus_tag="VNZ06_13440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWW21798.1"
                     /translation="MQKEDPAAEVPEPFRGPATWSLDLRTGEMHSSPARARLMGYDPQ
                     SVVPSSQWWDRALHPDERLAVYTARSRHLQGTDSGYRSEYRVQRQDGEWMWLLEIGCI
                     ERDNAGSAISVHGVLLDITVDREAQFRTQQRLERLSTIFDYIYQLTGLLSPAGVVLEI
                     NRSALRAAEASRAEALGMPLWETVLWQHQSAGETERLRDGIRRASRGEFVRFQMQADH
                     PQGGQKRIIDFSLLPVLDDEGAVTSILSEGRDVTDIVTAQAAVRNMEDRLAIAASTAN
                     LGLWDVDLRTGEGWHNDYCWTMLGYEPHEVATGIQQWLSMIHPDDLAQTILLMQDQRD
                     TGGDFRAEYRMRAKDGSWRWIHCFGRTVANPDPNAPKRMAGVHLDVTERREAEIRSHA
                     AERLESVGKLAAGVAHEINTPVQFVSDSIYFVRDGLYELLALTDRLRMLASRDQCNAN
                     SVAALSTHLPYLVENLPKALERSLDGLKRVSEIVSSMRELAHPDRPEMSDIDVNHVIQ
                     NAMVVARSEYKYVAEIETELTPLPPVRGHAGELNQVILNLLVNASHAISDVVGESQQK
                     GLIRITTQLDGDSVLISVSDTGSGIPANIQHRIFEPFFTTKDVGRGTGQGLAISHNII
                     VKRHGGSIEFDSTVGQATVFRVRLPYDRKAAEQVA"
     gene            2950..3951
                     /locus_tag="VNZ06_13445"
     CDS             2950..3951
                     /locus_tag="VNZ06_13445"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWW21799.1"
                     /translation="MKAHNVATLTARYVQFASGLALGLSLGLAAPARATVYELSDPDQ
                     QVVGEDQTLATVYEDTLYDLARQYSLGSEEVIRVNPGIDPWLPGAGKQVIIPGRHILP
                     PGPHEGIIVNLPEHRLYYFPKPQRGGKHPIVISYPVSIGKMDWHTPIGVTHVIQKEKD
                     PVWFPPESVRKEHAANGDPLPVRVPAGPDNPLGAFAMRLAAGNGTYMIHGTNNPIAVG
                     LAVTHGCIRMYPEDVSQLFPLIPVGTQVRLINEPVKVAWVNGELLLEAHPPVDAEGQS
                     FEPDVDQFAELLKTAVGDNTVAIHWDYARDVLQKADGILATVGLEADLSGVRNVASQ"
     gene            4127..4345
                     /locus_tag="VNZ06_13450"
     CDS             4127..4345
                     /locus_tag="VNZ06_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21800.1"
                     /translation="MSERENESFDLDEEFVVETEDSTDYDGLLARVDKRARAQGKRGK
                     AAWSRLEEVLADRKLEKDLRDIFDDEEV"
     gene            4400..>4778
                     /locus_tag="VNZ06_13455"
     CDS             4400..>4778
                     /locus_tag="VNZ06_13455"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21801.1"
                     /translation="MAPGESSWVVLKFGGTSVSTPSNWRNIAQVVRGRLADGARVMLV
                     HSALTGVTDRLEKLLVAALGGAHEPLLRVIEERHCELAVELGVGVSPELDGYFAVLRQ
                     MAAGIALMNEVSDRTRARVMACGE"
BASE COUNT          865 a         1516 c         1529 g          868 t
ORIGIN      
        1 tcgattcgcg cgcgatctgg atgacctccg cgaatgtctc cggcgagcac atcgcgagca
       61 ccgcctgccc gatgtccagc agcagcgcgg cggtgaaggc ctcatcggcg tggcgtgagt
      121 ccttcagcaa tgagcgtgcg aagtgcgcct tgcgcagcga acgggcctgc aggtcgcgca
      181 tcagcttgag tgcggccggt gaggcgtcga gcgcgttgaa cacgcaggcc gacagcgcca
      241 gcgacttgat catttccatg ccgacgaaag tgaccgccgc cgggatcgag gtgatcgcct
      301 gccggcggcg gaagaatgac gagttgacga tctgcagggt cttgacgcac agcgccgtgt
      361 ccttgctgac gatgcgtgag atgtccgcgg tgtgcgtatt cggctccgtc atcgcatcgg
      421 acaggtcctg gaaagtctgt gcggtggcag gcagcgcatt cagacgaccc accagcgcgc
      481 ggatgccctg atgatgcaga atcgaattga gcgcacaggc gcgctcgatc acctcgcgca
      541 aggcatcgcc cacgatcggt ttgctcagga actggtgagt caccggcatc gcgcgcatga
      601 agtcggcgtc ctccatcccc gcggacatgc acacgcgcac cacgttcgga aactgattgc
      661 gcacctgcgc cagcagggtg gggccgtcca tgtccggcat gcgcaggtcg ctggcgatcg
      721 cgtcgaactc gccgccctcg agggccgcca gcgcgccccg gccggaatcg acgaacacca
      781 cgtcgtaatg caggcgcgcg tgactgatct cgcgccggat ctgctcgagc gccactggat
      841 cgtcatcgac gaacagcagg cgcatcatgc gacttgctcc gcggccttgc gatcgtacgg
      901 cagccgcacg cggaacaccg tagcctggcc gaccgtgctg tcaaactcga tcgagccgcc
      961 atgacgcttg acgatgatgt tgtgcgagat cgccagcccc tgaccggtgc cgcgaccgac
     1021 gtccttggtg gtgaagaacg gctcgaagat gcgatgctgg atgttcgcgg gaatgcccga
     1081 gccggtatcg ctgacgctga tcagcacact gtcgccgtcg agctgggttg tgatccggat
     1141 cagtcccttc tgctggctct cgccgacgac gtcgctgatg gcgtgtgagg cattcaccag
     1201 cagattcagg atcacctgat tgagctctcc ggcatgaccg cgcaccggcg gcagcggcgt
     1261 cagctcggtc tcgatctcgg cgacgtactt gtactccgag cgcgccacca ccatcgcgtt
     1321 ctggatgacg tgattgacgt cgatgtcgga catctccggc cggtccggat gcgccagttc
     1381 gcgcatcgag ctgacgattt ccgatacccg cttcagcccg tcgagcgagc gctcgagcgc
     1441 cttcggcaga ttctcaacca ggtagggcag gtgcgtgctc agcgccgcca ccgaattggc
     1501 gttgcactga tcgcgtgagg ccagcatgcg caaccggtcg gtgagcgcca gcaattcata
     1561 cagcccgtcg cgaacgaaat agatgctatc gctgacgaac tgcaccggcg tgttgatctc
     1621 gtgtgccacg ccagcggcga gtttgcccac cgactcgagc cgctccgccg catgcgagcg
     1681 gatctctgct tcgcgccgtt cagtcacatc cagatgcacg ccagccatgc gcttgggcgc
     1741 gttcggatcg ggattggcca ccgtgcggcc gaagcaatgg atccagcgcc acgagccgtc
     1801 cttggcgcgc atgcggtact cggcgcggaa atcaccgccg gtgtcccgct gatcctgcat
     1861 cagcagtatc gtctgggcca ggtcatccgg gtggatcatg gaaagccact gctggatgcc
     1921 ggtggcgact tcgtgcggct cgtagcccag catcgtccag cagtaatcgt tgtgccagcc
     1981 ctcgccggta cgcagatcca cgtcccacag tccaaggttc gccgtgctgg cggcgatggc
     2041 cagccggtcc tccatgttgc gcactgccgc ctgcgcagtg acgatgtcag tgacgtcgcg
     2101 gccttcggac aggatcgagg tgactgcgcc ctcatcgtcc agcaccggca gcagcgagaa
     2161 gtcgatgatg cgtttctgcc cgccctgcgg gtggtcggcc tgcatctgaa agcgcacgaa
     2221 ttcaccacgg ctcgcgcgtc tgatcccatc gcgcaggcgc tcggtttcgc cggccgactg
     2281 atgttgccac aacacggtct cccacagcgg catgccgagc gcttcggcgc gcgaggcttc
     2341 agcggccctg agtgcggagc gattgatctc gagcacgacg ccggccgggg agagcaggcc
     2401 ggtgagctga tagatgtagt cgaaaatggt gctcaggcgc tcgagccgct gctgggtgcg
     2461 aaactgcgcc tcgcgatcca ccgtgatgtc gagcagcacg ccgtgtacgc tgatcgcgct
     2521 gccggcgttg tcgcgctcga tgcagccgat ctcgagcagc cacatccatt cgccatcctg
     2581 gcgctggacg cgatactcgc tgcgatagcc gctgtcagtg ccctgcagat gtcgtgagcg
     2641 ggcggtgtag accgcaaggc gctcatccgg gtgcagcgcg cgatcccacc actgcgagct
     2701 cggtacgacc gactgcgggt cgtagcccat cagccgcgcg cgcgcgggac tcgagtgcat
     2761 ctcaccggtg cgcagatcca ggctccaggt agccggtccg cgaaacggtt cgggcacttc
     2821 ggcagcgggg tcctcctttt gcataccggc gttatcggcg ctcgggagcg ggacttgagg
     2881 aactaagtcc gcaatctgcc tcgggaaacc atgcgcaatc aacggctcgt gcgtggtact
     2941 ctagaaatcg tgaaagcaca taacgtcgca acgctgacag cccgttatgt ccagttcgcg
     3001 tcggggcttg ctttgggcct gagcctgggg ctcgctgcgc ccgcccgcgc aacggtctac
     3061 gagctgtcgg accctgatca gcaggtggtc ggtgaggatc agaccctcgc caccgtctat
     3121 gaagacactc tctacgatct ggcgcgccaa tacagcctgg gctccgagga ggtcatccgc
     3181 gtcaatccgg gaatcgaccc ctggctgccg ggtgccggca agcaggtgat cattccgggg
     3241 cggcacatcc tgccgccggg tccgcacgaa gggatcatcg tcaatctgcc cgagcaccgt
     3301 ctgtactatt ttccgaagcc tcagcgcggc ggcaagcatc cgatcgtcat cagctatccg
     3361 gtcagcatcg gcaagatgga ctggcacacg ccgatcggtg tcacgcacgt gatccagaaa
     3421 gaaaaggatc cggtgtggtt tccgcctgaa tcggtgcgca aggagcatgc cgccaacggc
     3481 gatccgctgc ccgtgcgcgt gccggccggt cccgacaatc cgctcggggc attcgcaatg
     3541 cgactggcgg ccggcaacgg cacctacatg atccacggta ccaacaatcc gatcgcggtc
     3601 gggctggcgg tcacgcatgg ctgcatccgc atgtacccgg aagatgtgtc acagcttttc
     3661 ccactcattc cggtcggcac ccaggtgcgg ctcatcaatg agccggtgaa agtggcctgg
     3721 gtgaacgggg agctgctgct ggaggcccac ccgccggtgg acgccgaagg ccagagcttc
     3781 gaaccggacg tcgatcagtt cgcggaattg ctaaaaaccg ctgtcggcga caacaccgtg
     3841 gctatccact gggactacgc tcgagacgtc ctgcaaaagg ccgatggcat attggccacc
     3901 gtgggtctgg aagccgatct gtccggtgtc cggaacgtcg cctcccaata gcccgccaga
     3961 gggccctgac gcaggcgtgg gtcagtggcc ggaacaagct gccggccctg ccggtctacc
     4021 ctatggttga acagaagtac agatcagcta tagttcgcag cctgtaggtt tgacctattt
     4081 gtacttaatt aattgattta tctggcattt attcagcaga aatacgatgt ccgaacgcga
     4141 aaacgagagc ttcgacctgg atgaggaatt tgtcgtcgag acggaagaca gcaccgacta
     4201 tgacggactg ctggcccggg tagacaagcg cgcccgcgcc caaggcaagc gcggcaaggc
     4261 ggcctggagc cgtctggaag aggtgctcgc cgaccgcaaa ctcgagaaag acctgcgcga
     4321 tattttcgac gacgaagaag tttgatcgtc cgcgcggtca catctttagg ctccgcgcca
     4381 ctttaggcga gtaatgtcca tggctcctgg cgagtcttcc tgggtcgtgc tcaagttcgg
     4441 cggcacgagc gtttccactc cttccaattg gcgcaacatc gcgcaggtcg tgcgagggcg
     4501 cctggccgac ggcgcgcgcg tcatgctggt gcattcggcg ctcaccggtg tcaccgaccg
     4561 tctggaaaaa ctattggtcg ccgcccttgg tggtgcccac gagccgctgc tgcgggtgat
     4621 cgaggaacgt cactgcgaac tggcggtcga gctcggcgtc ggcgtcagcc ccgaactgga
     4681 tgggtacttt gcggtgctgc gacagatggc agccggcatc gcgctgatga acgaagtcag
     4741 cgatcgcacg cgagcgcgcg tcatggcctg cggcgaac
//
LOCUS       DATMRM010000443         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2424653, whole genome shotgun sequence.
ACCESSION   DATMRM010000443 DATMRM010000000
VERSION     DATMRM010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2424653"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VNZ06_13460"
     CDS             complement(<1..843)
                     /locus_tag="VNZ06_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWW21802.1"
                     /translation="MKIVRPTLAAALILALGSNAHASVVLAEKVLRVAYNEDVATFDP
                     DGAIILFGLDVSRVLYEGLVQYKPGTTQIVGWLAPSWSISPDGRTYRFTLRDGVVFHD
                     GQAMTAADVLVYFRRRQTPSLPLSYFLDGVESMSAPDSHTFVITLKAPQPAFLDRLAS
                     AWAPKVIGPEALVDHAGGDLGVGWLNEHADGTGPFKLLEFARGQRYVLVRNTNYWGPK
                     PYFDKIEILIVPSTNQQMLMLERGEIDIVEHGYPFDQLGKLPPGLKVDAHDGLGLEMA
                     YFNQT"
     gene            complement(855..1646)
                     /locus_tag="VNZ06_13465"
     CDS             complement(855..1646)
                     /locus_tag="VNZ06_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWW21803.1"
                     /translation="MPRIEAAAVCMLFVITVIAFAAPYLAPADPVLKIGEPYQAPSLH
                     YLFGTDEIGRDMLARIIYGVRLTWLPSLVIIAAGVAIGTAVGVGGAIAGGWVDLLLAR
                     LTDLFIVIPSTMVALAAVAALGPGIWHTVATMSVFWWPWYARIVRSEVKAVAGRPHAE
                     AARVSGVRGARLVMRYLLPAALPTVLVTATLDVANVILVLSLFSFLGLGAPAPAPELG
                     AMTARSLSSLTTHWWIPILPAIAIFLLVVTANLAGDGLRSLMKTV"
     gene            complement(1682..>2521)
                     /locus_tag="VNZ06_13470"
     CDS             complement(1682..>2521)
                     /locus_tag="VNZ06_13470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWW21804.1"
                     /translation="SCFRTGDVTPALWASASAQWPETAAAKPGILFEMAMVEKAFSLP
                     RRLFAGAHRSVRALDKINLRIYRGESVAVVGCSGSGKSTLLRAAAGLLRPDSGDLIHD
                     RGEPPQAIFQDAKGSLTPWLSIGEQISERLRPLRLTKADREARVLSALEQVGLDTRLG
                     RARARDLSGGQCQRAALARAIVVPPSLLLCDEAISAIDVTSAAAMLNLIGSLRRRLGM
                     AVLFVTHDLAAARFIADRIVVMSCGGIVEMGDAERIMTAPVHPATVALIEAMPDRLAR
                     AVA"
BASE COUNT          525 a          826 c          750 g          420 t
ORIGIN      
        1 cgtctggttg aaataggcca tctccaggcc cagcccatcg tgcgcatcga ccttcagtcc
       61 gggtggaagc ttgccgagct gatcgaacgg ataaccatgt tcaacgatat cgatctcccc
      121 gcgttccagc atcagcatct gttggttggt cgaggggacg atcaatatct cgatcttgtc
      181 gaaatagggc ttcggacccc agtaattcgt gttacggacc agcacatagc gctggcctcg
      241 tgcaaactcg agcagcttga atggaccggt gccgtccgcg tgttcgttga gccagccgac
      301 acctagatcg ccgccggcgt gatcgaccag ggcttcgggc ccgatcacct ttggcgccca
      361 cgcgctcgcc agtcggtcga gaaaggccgg ctgcggcgcc tttagcgtga tcacgaaagt
      421 atgcgagtcc ggtgccgaca tactttctac cccatccagg aaataggaca ggggcagact
      481 cggagtctgc cgtcgccgga aatagaccag cacgtcggcc gccgtcatcg cttggccatc
      541 gtggaagacg acaccgtcgc gcaaagtgaa gcgataggtg cgaccgtcgg gcgaaatcga
      601 ccagctgggc gcgagccagc cgacgatctg cgtggtgcct ggcttatatt gaaccagtcc
      661 ttcatagagt acgcgtgata catctaggcc aaacaggatg atcgcgccgt ccggatcgaa
      721 cgtcgcgacg tcctcgttgt aagcgacccg cagcaccttc tcggcaagta caaccgacgc
      781 gtgcgcgttg gaccccaggg ccagaattaa tgcggccgcc aaggttggcc tcacgatctt
      841 cacgcgcaac ccccttagac cgtcttcata agcgagcgca gaccgtcacc cgctaaattg
      901 gccgtcacga caagcaggaa aatcgcgatc gcaggcagga tcgggatcca ccagtgggtc
      961 gtcaggctgg agagactgcg ggctgtcatg gcaccgagtt cgggcgcggg cgccggcgcg
     1021 cccagcccga ggaacgagaa tagcgacaac accaggatca cgttcgcaac atcgagcgtc
     1081 gccgttacca ataccgtcgg tagcgcggcg gggaggagat aacgcatcac aagccgcgct
     1141 ccgcgcacac cggacacccg tgccgcctcc gcatgggggc gtccggccac tgccttgact
     1201 tcgcttcgca cgatccgcgc ataccagggc caccaaaaga cgctcatcgt cgcgacggtg
     1261 tgccagatgc cggggccgag cgcggcgacg gcggccaacg caaccatggt cgatgggatg
     1321 acaatgaaca gatcggtcag gcgcgcgagc agaagatcta cccagccacc ggcaatagcg
     1381 cccccgacac caacggctgt tccgatggca accccggcag cgatgatgac caagctaggc
     1441 agccaggtaa gtcgcacgcc gtagataatc ctggccagca tgtcgcgtcc gatctcatcg
     1501 gtgccgaaca gataatgcag cgagggcgcc tggtacggtt cgccgatttt cagcaccggg
     1561 tcggcaggcg caagataggg cgcggcgaac gcgatcaccg taatcacaaa cagcatgcac
     1621 acggccgccg cctcgatgcg cggcatctgc atcagtgtct tgcgcgcgcg cgcgcctatc
     1681 attacgcgac ggcccgcgcc aaccggtcag gcatcgcctc gatcagcgct accgtggcgg
     1741 ggtgtaccgg cgccgtcatg atcctttctg catcccccat ctcgacgatc cctccgcacg
     1801 acatcaccac gatccggtct gctatgaacc tcgcggccgc aaggtcatga gttacaaaca
     1861 ggaccgccat accgagtctg cggcgcaagc tgccaatcag gttcaacatg gccgcggccg
     1921 atgtcacatc gatggcgctg atcgcctcat cgcacagcag caggctgggt ggtaccacga
     1981 tcgcccgagc cagagcagct ctttggcatt gaccgcccga cagatcgcgc gcccgtgcgc
     2041 ggcccagccg cgtgtccagt ccgacctgct ccagcgccga caagacccgc gcttcgcgat
     2101 ccgccttggt caggcgcaat ggccgcagcc gttcgctgat ttgctcgccg atcgatagcc
     2161 agggagtcag cgaccccttc gcatcctgga agattgcctg gggcggctcg cccctgtcgt
     2221 ggattagatc gccgctgtct ggcctcagca gccctgctgc agcgcgcagt aaagttgatt
     2281 tacccgagcc gctgcaaccg acaactgcaa cagattcgcc ccgatagata cgcaggttga
     2341 tcttatccaa agcgcgaacc gaacgatgag caccggcgaa cagacggcgg ggtagcgaga
     2401 atgccttctc caccatcgcc atctcgaaga gaattccggg cttagcggca gcagtctcgg
     2461 gccattgtgc cgacgcgctc gcccagagcg caggagtgac atcaccggtg cgaaagcacg
     2521 a
//
LOCUS       DATMRM010000444         3557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4296475, whole genome shotgun sequence.
ACCESSION   DATMRM010000444 DATMRM010000000
VERSION     DATMRM010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4296475"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VNZ06_13475"
     CDS             <1..937
                     /locus_tag="VNZ06_13475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW21805.1"
                     /translation="TREIVLAMIAPQIALLAAGLSLAWVNVNRGLKPLTDLAKQIEAR
                     DHENLAPVSEHGLPVEARALVLRLNELFVRVREGIVSQERFVADAAHQLRTPLAALVL
                     HADAAERAGETDAQRRALRSLRISADRAARLSGQLLVLMRAGPAAAAAVQFSEVDLTQ
                     LVRRVGEEWVPQMLARGVDFGLAVPATAVPISASDALLAELLSNLLDNARHYGKPSGR
                     VTLGVVALPTPALYVEDDGPGIDPAEQPRIFERFYRSPGTAGDGCGLGLAIVKEIAEL
                     HHASVHVHSDPLVGGTRVSVVFGAGAGSGAPRDGG"
     gene            complement(938..1252)
                     /locus_tag="VNZ06_13480"
     CDS             complement(938..1252)
                     /locus_tag="VNZ06_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21806.1"
                     /translation="MKRLVITGMVLFAQLAAAPVFAGNCSEPTPFTEIPSGASATREQ
                     MLAAQRAMKAYDIAVKAFSDCLKDAGDTSNRANVAIDKLEHMAQRFNVELHAFKERNG
                     AS"
     gene            complement(1496..1888)
                     /locus_tag="VNZ06_13485"
     CDS             complement(1496..1888)
                     /locus_tag="VNZ06_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21807.1"
                     /translation="MSTPNRGFDERMGPRRGRALLLVLAVLSLLVIVTVSAWLVLDGL
                     RHERSIQSIGIGVLSLMAILLWLRIVRARWLSLQVQTYVAPSASEETGVWGVGGPGMR
                     TPGATGIYTALQGGGRIDDPPGEGPRPR"
     gene            complement(1916..>3557)
                     /gene="ggt"
                     /locus_tag="VNZ06_13490"
     CDS             complement(1916..>3557)
                     /gene="ggt"
                     /locus_tag="VNZ06_13490"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWW21808.1"
                     /translation="GWLLLRAAGAHDAATLDAAAVASPDRYGALAAQEVLKAGGNAVD
                     AAMATGFALAVTYPEAGNLGGGGFMTLYMSGKPYFLDYRETAPAAASPNMYLDAHGDP
                     IPDLSIVGNLSSSTPGSVRGLYEAHRRFAKLSWQRDLAPAIRYARDGFTVTAQLIAIR
                     DGSQPAFLSGTNFEHYFAGMHPGARFKQPELAATLERIAQGGVQEFYSGRTAQRLVEQ
                     MQRGPQKGLISLSDLARYHSMWREPVVGDWQGMTVITAPPPSSGGIALLQMLRMKADA
                     AALFKGVKLNSVQYIHLIAEIEKRVFADRAEYLGDPDFVTVPVAQLIAPDYLAARARQ
                     INPEKPSATLSVKPGLEKPQTSHYSIVDRWGNAVSNTYTLNGWFGSGVVVEGAGFLMN
                     DEMDDFSSKPGVPNMYGVVGWDANAIAPFKRPLSSMTPSILLKDGHVAMVLGTPGGSR
                     IFTSVFQVLINVYDYHLGLSAALAQFRFHHQLLPLDTVFDEPYRRIPPDVVQALTGRG
                     YRIENQDYNGDVEAIQIIGRRPIAAADPRARGVAMLVR"
BASE COUNT          636 a         1150 c         1159 g          612 t
ORIGIN      
        1 gacgcgcgag atcgtgctgg cgatgattgc gccgcagatc gcactgctgg cggcgggcct
       61 gagcctggcc tgggtcaacg tgaatcgcgg gctgaaaccc ctcaccgacc tggcgaagca
      121 gatcgaagca cgtgaccacg agaatctggc gcccgtatcc gaacacggcc tgccggtgga
      181 agcgcgcgcg ctggtgctgc ggctcaatga actgttcgtg cgcgtgcgag aaggaatcgt
      241 ctcgcaggag cgcttcgtcg cggatgccgc gcatcagctg cgaactccgc tggccgccct
      301 ggtcctgcac gccgatgccg cggagcgcgc cggggaaacc gacgcacagc gccgcgcgct
      361 gcgcagcctg cgcatttccg cggaccgcgc cgcgcgcctg agtgggcagc tgttggtgct
      421 gatgcgcgcg gggccggctg ccgcggcagc cgtgcagttc agcgaggtcg atctcacaca
      481 gctcgtgcgg cgggtgggcg aggaatgggt accgcagatg ctcgcacgcg gcgttgactt
      541 cgggctggca gtgcccgcaa cggcggtgcc gatttcagcc tccgacgcct tgttggctga
      601 gctgctgtcg aatctgctgg acaacgcgcg ccactacggc aaaccctccg ggcgggtcac
      661 gctgggtgtc gtggccctac cgaccccggc gctgtatgtc gaggacgacg gaccgggtat
      721 tgaccctgcc gagcagcccc gcatcttcga gcgcttctat cgcagcccgg gtacggcggg
      781 ggatggctgc ggtctcggac tggcgatcgt gaaggaaatt gccgagttac atcacgccag
      841 cgtgcatgtg cattccgacc cgctggtcgg cggtacacgt gtgagtgtgg tgttcggcgc
      901 gggcgcgggt tcgggtgcgc cccgcgacgg cggctgatca actggcgccg ttgcgttcct
      961 tgaaggcgtg cagctcgacg ttgaaacgct gagccatgtg ctccagtttg tcgatcgcca
     1021 cgttggcgcg gttgctggtg tcaccggcat ctttcaggca gtcagagaag gccttgacgg
     1081 cgatgtcata ggccttcatg gcacgctgcg ccgcaagcat ctgctcgcgg gtagcgctgg
     1141 caccgcttgg aatctcggtg aacggggtcg gttcgctgca gttgcccgcg aagacgggcg
     1201 ccgcggcgag ctgcgcgaac agtaccatcc cggtgatcac cagacgcttc atgcgtacgc
     1261 ttgactccag ccaacatcgg ccatgcggag ggcccgcatt gacaaaactt taagatttca
     1321 gacccccgtt gtgacgtgcc gcacgtctag actggtgcac acccttgtgt gaacttcgcc
     1381 gcaaaacgcg agatccgcgg tagacctcag agagcggcca cgggcggggc tgcgacgcag
     1441 catggatcga ctgccaggga gcggaaaggc aagtcgcgcg ccggtaggca cgaactcagc
     1501 gcggacgggg tccctcaccc ggagggtcgt cgatgcggcc gccaccctgc agagcggtgt
     1561 agataccggt cgcccccggc gtgcgcatcc ccggtccgcc gacgccccag acgccggtct
     1621 cctcgctggc agagggcgct acataggtct gcacctgcaa cgaaagccag cgggcacgca
     1681 cgatccgcag ccacagcagg atggccatca ggctcagcac accgataccg atgctctgga
     1741 tgctgcgttc atgtcgcaag ccatccagca ccagccaggc gctgaccgtg acgatgacca
     1801 ggagcgacag gactgcgagg accagcagca gcgctcggcc gcgtcgcggt cccatccgct
     1861 catcgaaacc ccggtttggc gtactcatga tccatcaccc ccaagcgtgc ccttctcagc
     1921 gcacgagcat cgcgactccg cgcgctcgcg ggtcggcggc ggctatcggc ctgcgtccaa
     1981 tgatctggat cgcctcgacg tcaccattgt agtcctggtt ctcgatacga taaccacgcc
     2041 cagttaacgc ttgcacgaca tcgggcggga tcctgcgata cggctcgtcg aacaccgtat
     2101 ccagcggcag caattgatga tggaagcgaa actgcgccag cgcggcactc aggcccaggt
     2161 gatagtcgta gacattgatc agcacctgaa acacggaggt gaagatcctc gagccgcctg
     2221 gggtaccgag caccatcgcg acgtggccat ccttcagtag gatactgggt gtcatcgatg
     2281 acagcggccg tttgaacggc gcgatcgcat tggcatccca tccgaccaca ccgtacatgt
     2341 tgggcacgcc gggtttggat gagaaatcat ccatctcatc gttcatcaga aagccggccc
     2401 cttcgaccac gacacccgag ccgaaccagc cgttgagggt ataggtgttg gagaccgcgt
     2461 tgccccagcg atcgacgatc gaatagtgcg aggtctgcgg cttctcgagg ccgggtttga
     2521 cgctcaacgt cgccgagggc ttctccggat tgatctggcg tgcgcgcgcc gccagatagt
     2581 ccggagcgat caactgtgcc acgggtaccg tcacaaaatc cggatcaccc aggtattcgg
     2641 cacgatcggc gaacactcgc ttttcgatct ccgcgatcag gtggatatat tgcactgaat
     2701 tgagcttcac acccttaaac agcgcggcgg catctgcttt catccgcagc atctgcagca
     2761 gtgcaatgcc gccggagctc ggcggcggtg cggtgatcac ggtcatcccc tgccagtcgc
     2821 cgaccaccgg ttcgcgccac atcgagtgat agcgtgccag atcgcttaag ctgatcaggc
     2881 ccttctgcgg cccgcgctgc atctgctcga ccagccgctg tgcggtgcgg cccgaataaa
     2941 actcctgcac cccaccctgc gcgatgcgtt cgagcgtggc ggcgagctcg ggctgtttga
     3001 agcgtgcgcc cggatgcata ccggcgaaat agtgctcgaa gttagtcccg ctgaggaacg
     3061 ctggctgact gccgtctcga atagcgatca gctgcgccgt caccgtgaaa ccatcgcgcg
     3121 catagcggat ggccggcgcc agatcgcgct gccaggacag cttcgcgaag cgacggtggg
     3181 cttcatagag cccacgcacc gatcccggcg tgctgctgga gagattgccg acgatactca
     3241 ggtcgggaat cggatcgccg tgcgcatcca gatacatgtt ggggctggcg gcggccggag
     3301 cggtctcacg atagtccagg aagtacggct tgccgctcat atagagcgtc atgaagccgc
     3361 cgccgcccag gttgccggcc tccggatagg tcaccgccag tgcgaaaccg gtggccatcg
     3421 cggcatcgac cgcgttgccg ccggctttga gtacttcctg ggcagcgagc gcgccgtagc
     3481 ggtcaggcga ggccacggca gcggcatcca gcgtggccgc atcgtgagcg ccggccgcgc
     3541 gcagcagcag ccaaccc
//
LOCUS       DATMRM010000445         3472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3034641, whole genome shotgun sequence.
ACCESSION   DATMRM010000445 DATMRM010000000
VERSION     DATMRM010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3034641"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VNZ06_13495"
     CDS             complement(<1..328)
                     /locus_tag="VNZ06_13495"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HWW21809.1"
                     /translation="MLTIQLSPNCSLTPRGAALFFGSLCAVTFSISTLMLAKGLWPIF
                     PFAGLEMLLLGWALRTSLRRRHFSQTITVTDEQVAVETSEREQHDRVVFPRHWAQVKL
                     RRADTHL"
     gene            complement(376..1749)
                     /locus_tag="VNZ06_13500"
     CDS             complement(376..1749)
                     /locus_tag="VNZ06_13500"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWW21810.1"
                     /translation="MQRFVKSAALIVAPFLLAGLIAGAAFRIDRPLRLGVNTVAHELC
                     SKTFISGLDPHEVFAESLALREGPKLIARYVRYEVDPLRHEVRAELRHLGLVRARAVF
                     YPGLGCIAIHGAPPADLPNPSQLMAARSNATAMLPDMAGPEVVETRDKGLQQALTQAF
                     TESDAREPKRTKAVVVVRDGRIIAERYAPGFGVDTPILGFSLTKSMMNALVGILVMQG
                     QLKITDPAPIPEWSASNDPRRAITIEQLMRMTSGLDLDEEDAGLADPSDRMLYLERDM
                     ATYARSAKLMVPPGTRFAYSSASTQILSSIVRAGAGPAAIDALEFADRELFAPLGMRH
                     VTVEVDSTGTMIGCNDMFASARDWARLGLLYVNDGIVGDRRILPSGWIDYSTAPTLGT
                     AYGAGFWTARGKGGLAEGLRSFALPSDSFLGSGNLGQRLLIIPSQHLVIVRLGDATGP
                     TNDAVHS"
     gene            complement(1784..2689)
                     /locus_tag="VNZ06_13505"
     CDS             complement(1784..2689)
                     /locus_tag="VNZ06_13505"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWW21811.1"
                     /translation="MSKPSASRTEAEFSLDRKALLRGFAKAAARSESSTRSLERLNAE
                     LLERLQYFELEPRHVLDLGAGVGSASLKLRERYPGAQVLAVDFVLAMLENAPRSWWPR
                     ARFHRVAADATRLPFSDGRFDLVYSNLLLPFCDRPHLVFREIARVLREGGLLVFATLG
                     PETLKELRSAWASVDDGAHVSVFPGLPQLGDALMHSGLREPVMDTEQHQLHYGDVRAL
                     MRELKDSGAQNSVGTRFHGLTGRGRLRAMVEAYEAVRTPSGIPATFEVIFGAAFASAP
                     GVQGAHQGGTEVAVPLANLKNPFRR"
     gene            complement(2751..3472)
                     /gene="bioB"
                     /locus_tag="VNZ06_13510"
                     /pseudo
     CDS             complement(2751..3472)
                     /gene="bioB"
                     /locus_tag="VNZ06_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009315099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="biotin synthase BioB"
BASE COUNT          683 a         1100 c         1072 g          617 t
ORIGIN      
        1 gcaggtgcgt gtcagcgcgg cgcagtttaa cctgcgccca atgccgcggg aacacgacgc
       61 gatcatgctg ttcgcgttcg ctggtctcca cggccacctg ttcgtccgtc acggtgatcg
      121 tctgcgaaaa gtgccgacgt cgcaggctgg tccgcagcgc ccatcccagc aacagcattt
      181 ccagtcccgc gaacgggaaa atgggccaca gccctttcgc cagcatcagc gtggaaatcg
      241 agaacgtgac cgcgcagagg ctgccgaaga acagcgccgc gcctcgcggc gtcagtgagc
      301 agttcggcga gagctgtatg gtcagcacgc gcggcatcat acgaaaatag gcttttctat
      361 cgcaaggtca gagacttagg agtgcaccgc atcgttggtc ggaccggtgg cgtcccccaa
      421 gcgcacgatg accagatgtt gcgacggaat gatcagcaga cgctgtccca ggttgccgga
      481 gcccagaaaa ctgtcggagg gcagagcaaa gctgcgcagc ccttctgcaa ggccgccttt
      541 accgcgagcg gtccagaagc ccgcaccgta ggcggtgccc agggtagggg cggtggaata
      601 gtcgatccat cctgaaggca ggatgcggcg atcccccaca atgccatcgt tcacgtagag
      661 caaccccagc cgggcccaat cgcgcgcact ggcaaacata tcgttgcagc cgatcatcgt
      721 gccggtcgag tccacttcga cggtcacatg ccgcatcccc agcggggcga ataattcacg
      781 gtctgcgaac tcaagcgcgt caatcgcggc ggggcccgcg ccagcacgca caatgctgga
      841 gaggatctgt gtgcttgcac tgctatacgc gaagcgcgtt ccgggtggga ccatcagctt
      901 agccgagcgc gcgtaagtgg ccatgtcgcg ctccagatag agcatgcggt ctgacgggtc
      961 ggcaagcccc gcatcctctt catccagatc gagcccgctg gtcatgcgca tgagctgttc
     1021 gatcgtgatg gcgcgacgcg gatcattgga cgcagaccat tcggggatcg gcgcaggatc
     1081 ggtaattttc agctgcccct gcatcaccag aatgcccacc agcgcattca tcatcgattt
     1141 ggtcagcgaa aaaccgagga tgggtgtgtc gacgccgaat ccgggtgcat agcgttccgc
     1201 aatgattcgg ccatctcgga cgaccacgac cgctttggtt ctctttggct cgcgcgcatc
     1261 ggattccgta aatgcctggg tcaatgcctg ctgcagcccc ttatcgcgcg tttccacgac
     1321 ctcggggcca gccatgtccg gcagcatcgc ggtcgcgttg gagcgcgcag ccatgagctg
     1381 cgacggattg ggcaggtccg cgggtggggc accgtgaatc gcgatgcaac cgagtccggg
     1441 atagaacacg gctcgggcgc gcaccaggcc aaggtgtcgg agctcggccc gtacttcatg
     1501 acgcaacggg tcgacctcat accgtacgta gcgggcgatc aatttcggtc cttcccgcaa
     1561 ggcgaggctc tcggcgaaaa cctcatgggg atcaagacct gaaataaatg tctttgagca
     1621 cagctcgtgg gccactgtgt tgacgcccag gcgcaacggg cggtcgatcc ggaatgcggc
     1681 gcccgcaatg aggccggcaa gcaggaaggg cgcgacaatg agtgccgcgg atttcacaaa
     1741 tctttgcatc gatcgcgcac catataacgg tgatattcgt caattatcgg cgaaatgggt
     1801 tcttaaggtt cgcaagtggg acggcgactt ccgtgccgcc ctgatgggca ccctggaccc
     1861 ccggtgcgct ggcgaaagcg gcaccaaaga tgacctcgaa cgtggctggg atacccgacg
     1921 gtgtgcgcac cgcttcgtag gcctccacca tcgcgcgtag ccgaccgcga ccggtcaggc
     1981 cgtgaaatcg ggtgccgacc gaattctggg cgccgctgtc cttgagttca cgcatcagcg
     2041 cgcgaacatc gccgtagtgc agctgatgct gttcggtatc catcacaggc tccctcagtc
     2101 cggagtgcat caacgcatca cccagctgag gcagaccggg aaacacactc acgtgagcgc
     2161 cgtcgtccac acttgcccac gccgagcgca gttccttcag cgtctccgga cccagggtcg
     2221 cgaataccaa cagtcctccc tccctgagta cgcgtgcgat ctctcgaaat accagatgcg
     2281 gtcgatcaca aaacggcagc aacagattgc tgtacaccag atcgaagcgc ccatcgctga
     2341 acggcagacg cgtggcgtcg gcggcaacgc gatgaaagcg ggcgcgcggc caccatgaac
     2401 gcggcgcgtt ctcgagcatc gccaggacga aatccaccgc gagtacctgc gcgccaggat
     2461 agcgctcgcg cagcttcagg ctggcgctgc caacgcccgc acccagatcc agcacatgac
     2521 gcggctcgag ctcaaagtac tgcaaacgct cgagcaattc ggcattcaat cgctccaggg
     2581 atcgagtgga tgattcactg cgcgcagccg ccttggcgaa gccgcgtagc agggcttttc
     2641 ggtcgagcga aaattcggcc tccgtgcggc ttgccgatgg cttgctcatg atgcggcagt
     2701 gtagccagcc cggctcaatc gcaggtcggc ttcgatgttc gctcggcgga ttcaggcctg
     2761 atccctaatc gcccgagcag ctcgcgatcg tgtgtcacat cgggattgtc ggtcgtcaac
     2821 aatttctcgc catagaagat cgaattcgcg ccggccagaa acgccagcgc ctgcagttca
     2881 tcgctcatct gggcccgccc ggcagacagg cgcacgtgtg ccttgggaat catcacgcgc
     2941 gccaccgcaa tggtgcggac gaaatcgaac ggatccacgt cggctgcctc tcccagcggc
     3001 gttcccggca cgcgcaccag ttggttgatc ggtacacttt ccggctgctg tggcagattg
     3061 gccagcgtga cgagcagtgc cgcgcgatcg cgctgcgtct cgcccatgcc gacgatgccg
     3121 ccgcaacaga cctggattcc cgcttcacgc acggcttcga gcgtgtcgag ccgatcctga
     3181 taggtgcggg tggaaatgat tttccggtag tactcttccg aagagtcgag gttgtggttg
     3241 tagaagtcga gacccgcgtg cttgagctgc cgggcctgcg ggggcgtgag catgccgagc
     3301 gtcacgcagg tctccagccc gagtgcccgc accgcgccaa ccatctgcga gattgcatcc
     3361 agttcgcgtg ctttgggtga tcgatacgcc gcgcccatgc agaaacgcgt ggcgccggcg
     3421 tcgcgggcag cgcgcgcccg ctccagaacc tgcgacagcg acatcaacgg ct
//
LOCUS       DATMRM010000446         5140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_235531, whole genome shotgun sequence.
ACCESSION   DATMRM010000446 DATMRM010000000
VERSION     DATMRM010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235531"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VNZ06_13515"
     CDS             complement(<1..190)
                     /locus_tag="VNZ06_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21812.1"
                     /translation="MGAVIGCGRMGVFPSESVRRFAPRCWFPLSHADAIQRHPDLQLG
                     ALCDTSPQALQRAAAAHPG"
     gene            complement(190..1209)
                     /locus_tag="VNZ06_13520"
     CDS             complement(190..1209)
                     /locus_tag="VNZ06_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWW21813.1"
                     /translation="MARIESRVAYLNAPRQLEFRSEPLDSGYVAAGQVLCETLVTAIT
                     PGTELAAYVGLPALREGTGYPRLQGYCNVARVLAVGAGVSHIAPGERVLSFASHRSHF
                     SVPADDVLQVLAAEARSEDMACAYLFHLGYNAVLRSGVRPGIRVLVIGLGVLGLTSVA
                     MAAIAGARVVGVSDHVLPQRLGLEFGAQAILPRADEGAIREALTADLADVVITTSMDW
                     GDWLLALQLAGNRATIACLGFPGRNRPPGEFNPLDSRYFYAKQLRIEAVGMSPERPEP
                     RGFLRFNERDNLDYVVALIRAGRLKPASLVAGTYAGKDLALAYEALLARERPAATCLL
                     RWAPG"
     gene            complement(1213..2460)
                     /locus_tag="VNZ06_13525"
     CDS             complement(1213..2460)
                     /locus_tag="VNZ06_13525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HWW21814.1"
                     /translation="MPPDGQLQFCARCLYGADHPLGLVIDEEGICSGCRVHEEKDRLD
                     WSERWQKLESLVRPFRSRDGRSYDCIVPVTGANDSYFIVHLVKERLKLNPLLVSYNKY
                     YNTALGIRNLANLRIRFNCDILLQNVNPDSVRRITRASLRHFGSLYWHCLAGQTVFPV
                     QTAVRYRIPLIIWGAHQGLEQVGMFSHEHEVQMTRRYRKDHDLMGVEAAGLLSGFDTL
                     TEADIWQYRYPDDRALEAVGVTGIYLGNYVRWDPKAQHELMMAQHAYHTARFARTFDC
                     YDHVDCFNYMNLHDQLKLYKHGYSKVTDHACREIRHGRITRVQGLALVRRHELRPVER
                     VELFCEWLGVTEGGLQFLMDLHRNPRFWHQTLPGRWEFRGWSTASPSSAPREDEGSAG
                     FTATGELDTRAPPSYIVVGKGYP"
     gene            complement(2464..3231)
                     /locus_tag="VNZ06_13530"
     CDS             complement(2464..3231)
                     /locus_tag="VNZ06_13530"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HWW21815.1"
                     /translation="MLKKRLVGVITVKDGRAVQSFGYGRHLPLGTAQCLAENLDRWGA
                     DEILVLCIDRSRRGLGPDLALLRQLGSLGLSTPLSFGGGIRTAADAAAVIQSGAERVC
                     LDALLRTNPAVVGEISTHIGAQAVIGVLPLAFADGTLQWLDYLSGRSTPLSVANLALF
                     ASRTVSEALVIDWRNEGRAGGFDERLVRHFPLAQVPLIAFGGVSEAAQLDTLLAHDQV
                     VAVGSGNFLAYREHAVQKLRQRSSARSLRSPIFGSEG"
     gene            complement(3224..3835)
                     /gene="hisH"
                     /locus_tag="VNZ06_13535"
     CDS             complement(3224..3835)
                     /gene="hisH"
                     /locus_tag="VNZ06_13535"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HWW21816.1"
                     /translation="MSRITVGIVDYGVGNHASVRHTLNELGLRCRVSDDPAVLDACNM
                     LLLPGVGAFRPAIEALREHHLDGYVTEQAARGRPLLGICLGMQLLAEASHEDGFTRGL
                     GVVPGEVVSLPAPRWHIGWNVIRTVPTEPLFRASDGEAFYFNHSYVYRGPAQLEVCRT
                     TASEEFVSGFRHGRIVGVQFHPEKSQAAGRTLLRQLISGLCDA"
     gene            complement(3832..4962)
                     /locus_tag="VNZ06_13540"
     CDS             complement(3832..4962)
                     /locus_tag="VNZ06_13540"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HWW21817.1"
                     /translation="MKYCVQCLQPDTRPNTLFTDAGICPACNYFAQLQQVDWRERYET
                     LQDLLERYRRKPGQYFDCIIGVSGGKDSTRQALFVRDKLGLRPLLACLSYPPQQVTER
                     GVDNISNLIELGFDTVLLSPAPETWRKLMRVSFDRFTNWARSTELALFSAVPQLAIRY
                     GIPLILWGENPGLQLGDLKTLGRTGYDGNNLRHMNTLSGGGMEWILEAGFSAQELLPY
                     RYPAPEEFDAAGLQIVYLGWFLGDWSLVNNGAYACAYGLAKRDDTVENTGDLYGLTSL
                     DEDWVTLNQMIKYYKFGFGRVTDYVNEEIRAQRMTRARGIDLVERYDDSCAPQYIASF
                     CEFIDISVEQFWSQVHAAVNRELFSVHVDGSIRRRFQVGVGL"
     gene            complement(4959..>5140)
                     /locus_tag="VNZ06_13545"
     CDS             complement(4959..>5140)
                     /locus_tag="VNZ06_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21818.1"
                     /translation="PLASDLAWADAVAGLQSYALVVALASSRRAISYLPPGAPPCALR
                     YPGVERLATLPKVSP"
BASE COUNT          952 a         1717 c         1638 g          833 t
ORIGIN      
        1 tgcccggatg ggctgcggcc gcgcgctgca gggcctgcgg gctggtgtcg cacagcgcac
       61 cgagctgcag atccgggtgg cgttgaatgg cgtcggcgtg actcaagggg aaccagcagc
      121 gtggtgcaaa tcgtcgcacc gactcgctgg gaaacactcc catccgtccg caaccgatga
      181 cagcacccat catcccggcg cccaccgcag caggcaggtg gccgccggac gctcgcgcgc
      241 cagcagcgcc tcgtaggcga gcgcgaggtc tttgccggcg taggtaccgg ctaccagcga
      301 ggccggtttc aaccgcccgg cgcggatcag cgcaactacg taatcaaggt tgtcgcgctc
      361 gttgaacctg agaaacccac gaggttctgg ccgctcgggc gacatgccga ccgcttcgat
      421 gcgcagctgt ttggcgtaga agtaccggct gtcgagcgga ttgaattccc cgggcggccg
      481 gtttctgcct gggaagccaa gacacgcgat agttgcgcga ttgccggcaa gctgcagggc
      541 gagcagccag tcaccccaat ccatgctggt cgtgataacg acgtccgcaa gatcagcggt
      601 cagggcctcg cggatggcgc cctcgtcggc tctgggaaga atcgcctgcg cgccgaattc
      661 cagacccagc cgttgtggca gcacgtggtc cgagaccccg acgacgcgcg cgccggcgat
      721 cgcagccatc gcaaccgagg taagcccgag cacgccgagg ccaatcacca gcacgcggat
      781 gcccgggcga acgccggatc tgagtaccgc gttgtagccg aggtgaaaga ggtacgcgca
      841 tgccatgtcc tcgctcctgg cctcagcagc cagaacctgc agcacatcat cagcaggcac
      901 gctgaagtga ctgcggtgcg aggcgaaact gagtacccgc tcacccggcg cgatgtggct
      961 gacaccggcg ccgaccgcga gtacgcgggc gacgttgcaa tagccctgca accgcgggta
     1021 gccggttccc tcccgcagcg ccggcagccc gacataggcc gccagctccg taccgggggt
     1081 gattgccgtc accagggttt cgcaaagcac ctggccggcg gcgacatagc cgctgtcaag
     1141 aggctcgctg cgaaactcca gctgccgcgg agcattcaga tacgcaaccc tcgactcgat
     1201 gcgcgccatc gcctacggat atcccttgcc aaccacgatg tacgagggag gggcacgcgt
     1261 gtccagctct cccgtggcgg taaacccagc ggaaccctca tcttcccgcg gcgcactgct
     1321 ggggctcgcg gtgctccagc ccctgaactc ccagcggccc ggaagggtct ggtgccaaaa
     1381 gcgcggattg cggtgcagat ccatcagaaa ctgcagaccg ccctccgtca cgccgagcca
     1441 ttcgcaaaac agctcgaccc gctcaacggg gcgcagctcg tggcgccgta cgagcgccag
     1501 gccctgaacg cgggtgatgc gtccgtggcg tatctcgcgg cacgcgtggt cggtcacttt
     1561 cgaatagccg tgcttgtaca gtttcagctg gtcgtggaga ttcatgtagt tgaagcagtc
     1621 gacgtgatcg tagcaatcga aggtgcgcgc aaaacgcgcg gtatggtacg cgtgttgcgc
     1681 catcattaac tcatgttggg ccttgggatc ccagcgcacg tagttcccca ggtagatccc
     1741 cgtcacccca accgcctcca gcgcacggtc atccggatag cggtactgcc agatatccgc
     1801 ttcggtcagg gtatcgaagc ctgacaggag tcccgcggcc tcgacgccca tgaggtcgtg
     1861 atccttgcga tagcgccggg tcatctgcac ctcgtgctcg tgcgagaaca ttccaacctg
     1921 ctccagcccc tggtgcgcgc cccagatgat cagcgggatg cgatagcgca ccgcggtctg
     1981 cacgggaaat accgtctgtc cggcaaggca atgccagtac aggctgccga aatggcgcag
     2041 cgaggcgcgc gtgatccggc gtacggaatc cgggtttacg ttttgcagga ggatgtcgca
     2101 gttgaaacga atccgcaggt tcgccagatt ccgaatcccc agcgcggtgt tgtagtactt
     2161 gttgtagctc accagcagcg ggttgagctt caggcgctcc ttgaccaggt gcactataaa
     2221 ataagagtcg ttggcgccgg tcacgggcac gatgcagtcg taactgcgcc cgtcgcggct
     2281 gcgaaagggg cgcaccaggc tctcgagctt ctgccagcgt tcgctccagt cgagccggtc
     2341 cttctcttca tggacgcggc agcccgagca gatcccctcc tcatcgatga ccagcccgag
     2401 cggatgatcc gcgccgtaca ggcagcgcgc gcagaattgc aactggccgt ccggtggcat
     2461 gcgtcagcct tcgctgccga aaatcgggct gcgcagggat ctcgcgctgc tgcgctgtcg
     2521 caatttctgc accgcgtgct cccggtaggc cagaaagttg ccgctgccga ccgccacgac
     2581 ctggtcatgg gcgagcaggg tgtccagctg ggcagcctcg ctcacgccac cgaatgcaat
     2641 cagcggcacc tgcgcgagcg ggaagtgccg caccagccgt tcatcgaagc cgccggcgcg
     2701 tccctcgttt cgccagtcga tcaccagcgc ctcggacacc gtgcgactcg cgaacaacgc
     2761 cagatttgcc actgacagcg gcgtggaccg acccgacagg tagtcgagcc actgcagtgt
     2821 gccgtcggca aaagccagcg gcagcacgcc gatgactgcc tgcgcgccga tgtgcgtgga
     2881 aatttcgcca accaccgcag gattcgtgcg cagcagggca tccagacaca cccgttccgc
     2941 gccggattga atgactgccg cggcatctgc ggcggtgcgg attccgccac cgaagctcag
     3001 cggcgtggag agtcccagcg agccgagttg ccgcagcagc gcaagatccg gcccgagtcc
     3061 tcgccggctg cgatcaatgc acagcaccag gatctcgtcg gccccccagc gatcgagatt
     3121 ctcggcgaga cactgcgcgg tgccgagcgg caggtggcgg ccataaccga aggactgcac
     3181 ggcgcgcccg tccttgaccg tgatcacgcc gacgaggcgc ttcttaagca tcgcacaggc
     3241 ctgagatcag ctggcgcagc agcgtccggc ccgccgcctg actcttttcg ggatggaact
     3301 gcacgccgac gatgcgtccg tgccgaaagc ccgagacgaa ctcctcggag gcagtggtgc
     3361 ggcagacctc aagctgcgcg gggccgcggt agacatagga gtgattgaag tagaaggctt
     3421 ccccgtcgct cgcgcggaac agcggctccg tcggcaccgt gcggatcacg ttccagccga
     3481 tgtgccagcg cggtgccggc agactcacta cctcgccggg aaccacgccg agacccctgg
     3541 tgaaaccgtc ctcgtgcgag gcctcagcga gcagctgcat ccccaggcag atccccagca
     3601 acgggcggcc gcgtgcggcc tgctccgtga cgtagccgtc gaggtggtgc tcgcgcagcg
     3661 cctcgatcgc cggccggaag gcaccgacac ccggcagcag cagcatgttg caggcatcca
     3721 gcaccgcggg gtcgtcgctg acgcggcaac gcagtccaag ctcgttcagc gtgtggcgca
     3781 cggaggcgtg attgcccacc ccgtagtcga caataccgac cgtaatcctg ctcacagacc
     3841 caccccgacc tggaagcgtc gccggattga gccatcgacg tgaacgctga agagctcgcg
     3901 attcacggcc gcgtgcacct gcgaccagaa ctgctcgacg gagatgtcta tgaactcaca
     3961 gaaactggcg atgtactgcg gggcgcaact gtcatcgtag cgctccacca ggtcgatgcc
     4021 ccgcgcgcgc gtcatgcgtt gcgcacggat ttcctcgttc acgtagtcgg tgacgcgccc
     4081 gaagccgaac ttgtagtact tgatcatctg gttgagagtc acccagtcct cgtccagaga
     4141 cgtgagcccg tacaggtcac cggtgttctc caccgtgtcg tcgcgctttg ccaggccata
     4201 tgcgcacgcg taagcgccgt tgttcaccag cgaccagtcg ccgaggaacc agccgagata
     4261 cacgatctgc agtcccgccg cgtcaaactc ctcgggcgcc gggtagcgat agggcaacag
     4321 ttcttgggcg ctgaaaccgg cctccaggat ccactccatg ccgccgccgc ttaaggtgtt
     4381 catgtggcgc aggttgttgc catcgtagcc ggtgcgaccg agcgtcttga ggtcccctag
     4441 ctgcaggccc ggattctcgc cccacaggat cagcggaatg ccgtagcgga tggcgagctg
     4501 cggcaccgcg ctgaacaacg cgagctccgt ggagcgcgcc cagttggtaa accgatcgaa
     4561 ggagacgcgc atcagcttgc gccaggtttc cggcgccggc gacagcagca ccgtatcgaa
     4621 tccgagttcg atcaggttgg aaatgttatc cacaccgcgc tcggtcacct gctggggcgg
     4681 gtacgacagg cacgcgagca gcggccgcag tccgagcttg tcgcgcacga acagtgcctg
     4741 ccgcgtgctg tccttgccgc cgctcacgcc gatgatgcag tcgaagtact ggccgggctt
     4801 gcggcggtag cgttcgagca gatcctggag cgtctcgtag cgctcgcgcc agtccacctg
     4861 ctgaagctgc gcgaagtagt tgcacgccgg acaaatcccg gcatccgtaa acagcgtgtt
     4921 gggccgcgtg tcaggctgca gacactgcac gcagtatttc acgggctgac cttaggcagc
     4981 gtggccaggc gctcgacccc cgggtagcgc agcgcacacg gcggcgcgcc gggaggcagg
     5041 taactgatcg cacgccggct cgacgccagg gccactacca gcgcgtagga ctgcaagcct
     5101 gctacggcat ccgcccacgc cagatccgac gccagcggag
//
LOCUS       DATMRM010000447         2205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2428158, whole genome shotgun sequence.
ACCESSION   DATMRM010000447 DATMRM010000000
VERSION     DATMRM010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2428158"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VNZ06_13550"
     CDS             <1..288
                     /locus_tag="VNZ06_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21819.1"
                     /translation="LHTLRRMSVSVNGMLQAGKEPVLEGSIVKDLGTVWQQQLPHKAR
                     DLTAFLDSDQNDRAHFDEVLRYATTVVPKLTIQGGTTEVLRGIIARGLGLR"
     gene            307..>2205
                     /locus_tag="VNZ06_13555"
     CDS             307..>2205
                     /locus_tag="VNZ06_13555"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HWW21820.1"
                     /translation="MPDLSALLSPRSIAVVGAAPDVSILRGRTLKVLLRHPYIGKIYP
                     VSRSHREVQGLAAYASVDQIPAPVDLAVLIIPADQVPQELERCGSAGVRAAVILTSGF
                     AEQRGDQGMPLQRRLREIAARYDMAVCGPNAEGFVNAAANLCATFSPAVDNMEIPLLP
                     AWRRSGHVAVVAQSGGMGFAFFDRGRPKELPFSYIVTTGNEACLAAFDVVDYLLDEGR
                     SEVFLMFLEDIKHADSFRRAADKALRAGKPIIVAKVGGSAAGRRAAASHTGALAGQHE
                     IYRAMFRQYGIIEADTLDQMVDIAAAFSFYRTRLPAGKRVGIGTGSGGGGGWMADACA
                     AEGLEVPELDRPTRALIDPHLPAYGTSQNPVDGTAQAIRAIGYGELARLIALSDQVDA
                     VAMVITARNPEALEREKAHLQSISESGHKPILMWTYTLPAAASVRILSETGYPLFTDM
                     RHCAGALAALCAYRTLRERYLCARDTSTAYDRARAARIRQRLVQPVLCEYQVAPLLAD
                     YGIPTMESQLASSAAEAARCAVSLGAPVALKVQSPDITHKTDAGAVALRVWGDEAVQR
                     HYDEILQHARRHSPAARIDGVLVQRMAGAGLEVIVGVRRDPLFGPMMMLGMGGVGVEI
                     EHDLALAPA"
BASE COUNT          393 a          688 c          721 g          403 t
ORIGIN      
        1 ctgcacacgc tgcgacgcat gtcggtttcc gtcaacggca tgctgcaggc gggaaaggag
       61 ccggtactgg agggttcgat cgtcaaagat ctgggtacgg tctggcaaca gcaactaccg
      121 cacaaggcac gcgacctgac agcgttcctc gatagcgatc agaatgatcg cgcgcacttt
      181 gatgaggtgc tgcgttacgc caccaccgtg gtaccgaaac tcacgatcca gggcggtact
      241 accgaggtgt tacgcggcat catcgcgcgg ggcctgggtt tgcgttagtc cgcaccgtct
      301 taagctatgc ccgatctgtc tgcgctgttg tctccccgct cgatcgccgt cgtcggcgca
      361 gcccctgacg tcagcattct gcgaggacgt accctcaaag tgctgctgcg ccatccctac
      421 atcggcaaaa tctatccggt cagccgcagc caccgcgaag tgcaaggact ggcagcctac
      481 gcctcggtcg atcagatccc cgcgccggtc gatctcgcgg tgctcatcat tccggccgat
      541 caggtgccac aggagctcga gcgctgcggg agcgccggcg tgcgcgcagc cgtcattctg
      601 acttcggggt tcgcggagca gaggggcgat caaggcatgc cattgcagcg gcggctgcga
      661 gagatcgcag cgcgctatga catggccgtt tgcggaccca acgcggaagg cttcgtcaac
      721 gctgcggcaa atctgtgtgc caccttcagc ccggcggttg acaacatgga gataccgttg
      781 ctgccagcgt ggcggcgcag cggccatgtg gcggtggtcg cgcagagcgg cggcatggga
      841 ttcgcgttct tcgaccgcgg acgtccaaaa gaactgcctt tcagttacat cgtgaccacc
      901 gggaacgagg cctgtctggc ggccttcgat gtcgtggact acctgttgga tgaaggcaga
      961 tcggaagtgt ttctgatgtt cctggaggac atcaaacacg ctgacagctt tcgtcgcgcg
     1021 gccgacaagg cgctgcgcgc gggcaagccc atcatcgtgg cgaaagtggg aggcagcgcc
     1081 gcgggccggc gcgccgcggc ttcgcacacg ggcgccttgg cgggtcagca cgagatctat
     1141 cgggcgatgt ttcgccaata cggcatcatc gaggccgaca cgctggacca aatggtcgat
     1201 atcgccgcgg cgttttcgtt ctatcgaacg cgtttgccag cgggaaagcg cgtcggtatc
     1261 ggcaccggat ccggtggcgg cggcgggtgg atggccgatg cctgtgcggc ggaaggtctg
     1321 gaggtgcccg aactggatcg gccgacacgg gcgctgatcg acccacatct gccagcgtat
     1381 ggcacttcgc aaaatccggt agacggcacg gcgcaggcga tccgagcgat cggctacggg
     1441 gagctggccc ggctgattgc cttatccgat caggtcgatg cggtcgcaat ggtcatcact
     1501 gcgcgcaacc ccgaagcgct cgagcgtgag aaagcccatc tgcagagcat ctccgaatcc
     1561 ggccacaaac ccattctgat gtggacctac actttgcccg ctgcggcgtc ggtccggatt
     1621 ctgagcgaga ccggctaccc gctgttcacc gacatgcggc attgcgcagg tgcactcgcg
     1681 gcgttgtgcg cctatcgcac gcttcgcgag cgctacctct gcgcaagaga cacgagcacc
     1741 gcgtatgacc gggcccgagc cgcgcgcata cgacagcgac tggtccagcc agtgctgtgt
     1801 gaataccagg ttgccccgct cctggctgac tacggcatcc cgactatgga gtcgcagctg
     1861 gcgtcgagcg ccgcggaagc ggcgcgctgc gcagtctcac tcggcgcacc ggtagccttg
     1921 aaagtgcagt cgccggacat tacgcacaaa accgatgccg gcgcggtcgc tttgcgcgtt
     1981 tggggcgatg aggccgttca gcggcactac gatgaaattc ttcagcacgc gcgccgccat
     2041 tcgcccgcag cccgcatcga tggcgtgttg gtccagcgaa tggcaggcgc cggtctcgag
     2101 gtgatcgttg gcgtccgccg tgatccactg tttggcccaa tgatgatgct cggcatgggt
     2161 ggtgtcggag tcgagatcga acacgacctg gcgcttgccc ccgcc
//
LOCUS       DATMRM010000448         2456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_589311, whole genome shotgun sequence.
ACCESSION   DATMRM010000448 DATMRM010000000
VERSION     DATMRM010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589311"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VNZ06_13560"
     CDS             <1..184
                     /locus_tag="VNZ06_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21821.1"
                     /translation="TDYLTVVTSQTAVLQTQLEALNLDTLQLRASVDLIRALGGGWDD
                     SMTAQSGKAMDSQHPG"
     gene            complement(356..778)
                     /locus_tag="VNZ06_13565"
     CDS             complement(356..778)
                     /locus_tag="VNZ06_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWW21822.1"
                     /translation="MVKTWFITGISSGLGRNMTEQLLTRGDRVAGTARKLHALDGLKS
                     RYANQLSVAALDVTDGAAVRRVIDGAFNSLGRIDVIVNNAGYGLFGAAEELNDEQMRQ
                     QIDTNLVGSIQVIRAALPHLRAQGGGRILGDCKQRRDI"
     gene            complement(802..1710)
                     /locus_tag="VNZ06_13570"
     CDS             complement(802..1710)
                     /locus_tag="VNZ06_13570"
                     /inference="COORDINATES: protein motif:HMM:NF007695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HWW21823.1"
                     /translation="MSLLERRSTMSTINRTGTFALGDRTVVRLGYGAMQLAGPGVFGP
                     PRDTDAALTVLREAVESGVNHIDTSDYYGPHITNQLIRRALHPYRDDLVIVTKIGARR
                     GTDGSWLQAFSPQELTQAVHDNLRNLGLDVLDVVNLRSMLGAHGPAEGSLAAPLSVVA
                     ELQREGLVRHIGLSNVTPAQIEEGRRIVKIVCVQNHYNLAHRADDALIDDLAINGIAY
                     VPFFPLGGFSPLQSSILSDVAQRLGATAMQVALAWLLRRAPNILLIPGTSSVAHLREN
                     LAVAELDLPNDAVVALDGIGAHAGAA"
     gene            complement(2117..>2456)
                     /locus_tag="VNZ06_13575"
     CDS             complement(2117..>2456)
                     /locus_tag="VNZ06_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21824.1"
                     /translation="SAPHQNVTSVSLGLGVGGAAGLVYVGTVAAAMHGIRALYVPVRE
                     DWLWNAVLPTLAYATVLAMAILCRRKLEYSLYGVGAVSIVLLFIGIHNAWDVAVWNSI
                     SSQNDSKSDT"
BASE COUNT          483 a          768 c          751 g          454 t
ORIGIN      
        1 gaccgactac cttaccgtag ttacctccca aaccgcggtg ctgcagaccc agctggaggc
       61 actcaatctg gataccctgc agctacgcgc cagcgtcgac ctgatccgtg cgctcggcgg
      121 tggctgggac gattccatga cggcgcagag cggcaaggcg atggattcac agcaccccgg
      181 gtaggtcggc tcgacggatg ggttggatca tcaagaaacc cgtgcgcacc tcgcgtcgcc
      241 gagcgtcgga gcacaccaaa actcagccga cccgggtcgg tttactcgcc cccgatctgc
      301 tgtcccaagc aagagcaggg ttgccggctc ggggacagac gctggactac ccgtctcaaa
      361 tatcgcgccg ctgtttgcag tcgcccagaa tgcgcccgcc accctgtgcc cgcagatgcg
      421 gaagcgcggc gcggatcacc tgaatcgaac cgacgaggtt agtatcgatc tgttgcctca
      481 tctgctcatc attcagctct tctgcggcgc caaacagtcc gtagccggcg ttgttcacga
      541 tgacgtcgat gcgccccaaa gagttgaagg ctccatcgat cacccgccga acagccgcac
      601 catccgtgac gtccagcgcc gcaacgctga gctgattcgc atagcggctc ttgagtccat
      661 cgagcgcgtg cagtttgcgc gcagtcccgg cgactcgatc accgcgagtc agaagctgct
      721 ccgtcatgtt ccgtcccaga ccggaggaga ttccagtgat gaaccacgtc ttgaccatcg
      781 gaagcgctcc tcttactagc gtcaggcggc gccagcgtgc gcgccaatgc cgtctagggc
      841 cacgaccgca tcattgggca agtccaattc ggccacagct aggttttcgc gcaagtgcgc
      901 gaccgaggag gtacccggga ttaggagaat gttgggcgcg cggcgaagta gccatgcgag
      961 tgcaacttgc atggccgtgg caccgaggcg ctgagctaca tcggacaaga tggaagactg
     1021 cagcgggctg aacccgccca agggaaagaa cggcacataa gctatgccgt tgatggcaag
     1081 atcatcgatg agtgcgtcat cggcccgatg tgccaagttg tagtggttct gcacgcagac
     1141 gatcttaacg atccgccgtc cttcctcgat ttgcgcaggc gtaacattgc tcagtccgat
     1201 atgacgaacc agcccctcgc gctggagctc ggccactacg ctgagcggcg ccgcgagtga
     1261 gccttcggca ggtccatggg cacccaacat gctgcgaagg ttcaccacat cgagcacatc
     1321 caggcccagg ttgcgcaggt tgtcgtgcac cgcctgcgtc agctcttgcg gagagaaagc
     1381 ctgtagccac gatccgtccg tgccgcgccg ggcgccgatc ttggtcacga tgacgagatc
     1441 atcgcgatat ggatgcagtg ctctgcgaat cagctgattc gtgatatgcg ggccgtagta
     1501 atcactcgtg tcgatgtgat tcacaccgct ttccacggct tcgcgcagga cggtcagtgc
     1561 ggcatccgtg tctctgggcg gcccgaacac gccgggcccc gcgagctgca tggcgccgta
     1621 gccgagtctc accacggttc ggtcacccag ggcaaatgtg ccagttctgt tgatggtgga
     1681 catggtcgat ctcctttcga gcagggacac gatctagccg ctgtcggcct gcgcgatcat
     1741 aaggtccaag ccgcacgggc tgtgcgggaa aggcgaacaa tgaagacggc tctcgctgat
     1801 ctgaccgccg ttgtagcagt cgcacgcgcc aggggcttga gcgaagcggt gcgccgcctc
     1861 aaaacgcagc ttggcgtgcg gctcgtcaat agcagcggtc gcctcagtgg gcgagtaccg
     1921 gccagagaat ctcagcgatc caatccatga acacgcgcac gcgcttgcgg agattgcgcc
     1981 gatgcgcata tggcagcgag agggacatcg gctcgcgatt aaaagaggcg atcgcgccgg
     2041 attcgtcgtg gctctcgcac atggcgactc ggaatcgcac gccaggcgtg ggtgcggttc
     2101 atcgtcgcat tgccggctat gtgtccgact ttgagtcgtt ttggctgctg atgctgttcc
     2161 agactgccac gtcccatgcg ttgtggattc caataaagag caggacgatc gaaaccgcgc
     2221 cgacgccgta cagactgtat tcgagtttgc gcctgcacag gattgccatc gccaacacgg
     2281 tcgcgtaggc aagcgtgggc agtacggcat tccacagcca atcctcgcgc actggcacgt
     2341 acagcgcgcg gatgccgtgc atggcagcgg caacggtgcc gacgtatacc agccccgcgg
     2401 caccgccgac tcccaatccc aacgacacgc tggtcacgtt ctggtgcggt gcgctt
//
LOCUS       DATMRM010000449         6238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1843077, whole genome shotgun sequence.
ACCESSION   DATMRM010000449 DATMRM010000000
VERSION     DATMRM010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1843077"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VNZ06_13580"
     CDS             <1..472
                     /locus_tag="VNZ06_13580"
                     /inference="COORDINATES: protein motif:HMM:NF022144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2491 family protein"
                     /protein_id="HWW21825.1"
                     /translation="VDLNGSYLHRYYLDDDATWLQVKTDGGLDDAHISECLVWQYWDT
                     KTPANRDELKFIAGPSSLIGLPTYQVGAYLYQRVWGVSPGNTELIPLTERVFTDSDSI
                     SKYSCAHLSMLYRRSMTGTDRQEYVLISVEECPDSLQVVTSIGIDLLQADLTVT"
     gene            510..923
                     /locus_tag="VNZ06_13585"
     CDS             510..923
                     /locus_tag="VNZ06_13585"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HWW21826.1"
                     /translation="MNHPWTAALTGLPAFLLYFVTAIAVLLLFAYVYTRLTTHDEFGL
                     IRAGNGTAAIALGGSLIAFALPLCSALIHSVSLLDFLVWAAIAFVIQVATFFTVRIFD
                     SDLSARIARNEISAGIFVACSSIAVGLINAACMTP"
     gene            940..1488
                     /locus_tag="VNZ06_13590"
     CDS             940..1488
                     /locus_tag="VNZ06_13590"
                     /inference="COORDINATES: protein motif:HMM:NF018408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HWW21827.1"
                     /translation="MKRSRSIELTLMSAVPFLLVACDSPHGAASASLVYRSVADCIAG
                     GQVPEQTCRSEYDNAQALHQQQAPRFSSLEDCEARYGYDQCTRGHSADGSSIFLPLMA
                     GYLIGHAFGNHGYPYGYYYGGWHGQPIYRDRGDRAAWRTGTGAAVAYGEGIRGPAAMS
                     TAQTLSRGGFGMSSAARASWGG"
     gene            1492..2655
                     /locus_tag="VNZ06_13595"
     CDS             1492..2655
                     /locus_tag="VNZ06_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HWW21828.1"
                     /translation="MQRVSVAERSDWRKQAAQMGFHFHTLQGVPYWDESAYYRFSLNA
                     VENDLEKVTEEVHELCMDLVSRAVRDEVYLRRLAIPEHYWDFIRHSYRSGEPHIYGRM
                     DLAYDGNGPAKLYELNYDTPTSIYEAGVFQWLWLQQCSERGLIPRSSDQFNSLHDRLH
                     EVFRFIAAKVQSPLYFASVRDCVEDRATVDYLRDVAQQAGLTTHYLPIEDIGDTGDGA
                     YTDLDDQIIRAIFKLYPWEYMFADEFGRYLPTANTTWFEPPWKCILSNKAALALLWEL
                     HPGHPNLLATYIEQGSRSDLAAGWVRKPFYSREGANVEIATPAGQRLLNDGPYTEGPF
                     VRQAFHPLPRFGDNYALVGSWVIGDCAAGIGIREAAELITTDTSRFVPHIIAD"
     gene            complement(2684..3538)
                     /locus_tag="VNZ06_13600"
     CDS             complement(2684..3538)
                     /locus_tag="VNZ06_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013203448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HWW21829.1"
                     /translation="MKTPPALQPLIDDGVIDEVIRALKSGKEATVYLVRSGAQTRCAK
                     VYRDMRQRSFQRRAQYQEGRKPRGSRQTRAMSKRTRFGRSEQETAWKNAEVDALYRLQ
                     AAGVRVPKPYGYFNDVLVMELVTDSDGHPAPRLGEVELSSEQAREYHAFLIRQIVRML
                     HIGLIHGDLSEFNVLVDSHGPVIIDLPQVVNAAGNNAALAMLERDVNNIRGTLGRFAP
                     ELLCTEFAREMWSVYEQGELTADKKLTGQFARDETQADPESVLRVVNDAREEAMQREL
                     GRQGTIDR"
     gene            3791..5218
                     /locus_tag="VNZ06_13605"
     CDS             3791..5218
                     /locus_tag="VNZ06_13605"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWW21830.1"
                     /translation="MNSKCRALMFCLAALAAASAPVHTSAGDGLDAFGTGEQLHRLTP
                     GLSDPLMQMCEVPGRPLTLAMSVDLALCRNPTTRTAWAAAHQQAALLGEAKSAWLPDA
                     TATGTASRTIGQHLELTGEPSTSDQNTVDAAVNLSWTLYDFGARTGRISNARYLLDAA
                     AATAGSSVQQTILSVVQSYYGLAAADASLAAAKTTESVTAHSLEMARTLRSAGPGTLA
                     DVLQAETAYDQAVLAREQADAAAQAARGTYAVTLGLIADYGVKIDAAPVPAEVPAQKA
                     RMADLMAEAMRQRPDLAAAQSQRSAAEANVQVARAAGLPVITINAGGDYITSTGIPNQ
                     HYGLIGINVSIPLFTGFSTAYGVRRAKAALEESEVALEQTRLAVSLGVWNAYYALELA
                     NEQLGTTAGLARTAEDNERVALSSYQAGAGRFIDVVTAQTAAATARQVRISAELNWRV
                     SRAQLALALGRLSSVEPLQETRTIP"
     gene            complement(5221..>6238)
                     /locus_tag="VNZ06_13610"
     CDS             complement(5221..>6238)
                     /locus_tag="VNZ06_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="chitinase"
                     /protein_id="HWW21831.1"
                     /translation="TPIHLNEPSLAPPAASVRAASAPEWPPGTFAPYVDATLWPQLSL
                     LDASKALRMRHFRLGFVVARSAHDPAPTWGGTRSATSSFRLREINALRTLGGDVAIAF
                     GGQAGTELASAAKSGAELATRYRSVIDAYGARVIEFDLEGTALTDHQSIQRRSEALAL
                     LRKGMAAEQRPLEVWLTLPVTPTGLTPQGLEVIRSAIGQRVAIRGINAMTMDYGDAAA
                     PQPAGRMGSLAITAARAVRDQLRALYPANKTTMARTDLWQMIGITPMIGHNDIPSEVF
                     ELSDADQLLKFATEQHLGSLSLWSLNRDRACEPPAVTVSPSCSGIVQQPYEFARVLRA
                     FEAL"
BASE COUNT         1139 a         1950 c         1924 g         1225 t
ORIGIN      
        1 ggtggatctg aatggcagct atctgcaccg ctactatctg gatgacgatg ccacctggct
       61 gcaggtcaag actgacggcg gcttggacga tgcgcacatc tccgaatgtc tggtgtggca
      121 gtactgggac accaagacgc ccgcgaatcg cgacgagctc aagttcatcg ccggtcccag
      181 cagcctgatc ggtctgccta cctatcaggt cggcgcctac ctgtatcaac gcgtgtgggg
      241 ggtatcgcca ggaaataccg agctgatacc gctcaccgaa cgcgtattca ccgacagcga
      301 cagcatctca aaatattcct gcgcccactt gtcgatgcta taccgacggt caatgaccgg
      361 taccgaccga caggagtacg tgctgatctc cgtggaggaa tgtccggata gcctccaggt
      421 cgtcacctcg atcggcatcg atctgctgca ggccgatctg acggtcacct gaaatccaac
      481 tatcaacggt caatgaaagg gtctcgagca tgaatcatcc ttggaccgcc gcgctcactg
      541 ggttaccggc tttcctgctg tacttcgtca cagcgatcgc cgtgctgctg ctgtttgcgt
      601 acgtctatac ccgcctcacg acgcatgacg aatttggcct gatccgcgcc ggcaatggca
      661 ccgcggccat cgcactcggt ggctcactga tcgccttcgc tctgccactc tgcagcgcgc
      721 tgattcattc agtgtcgctg ctcgacttcc tcgtgtgggc ggcgatcgct ttcgtcatcc
      781 aggtcgccac atttttcacg gtgcggattt tcgattctga tctgtccgcg cgcatcgcgc
      841 gcaatgaaat ctctgccggc atctttgtcg cctgttcatc catcgccgtg ggactgatca
      901 acgcggcctg catgacaccg taattcatcg gagactctta tgaagcgctc gcgctcgatt
      961 gaattgacgc tgatgagtgc cgttcccttt ttgttggtgg cctgcgactc accgcatggc
     1021 gcggcttcgg cgtcgctggt ttaccggtcg gtcgcggact gcatcgccgg cgggcaggtc
     1081 cccgaacaga cctgccgcag cgagtatgac aacgcgcagg cgttacacca gcaacaggcg
     1141 cctaggtttt cttcgctcga ggactgcgag gcgcgatacg gctacgacca atgcacccgg
     1201 ggtcatagcg ccgacggctc gagcatcttc ctgcctctga tggccggcta tctgatcggc
     1261 catgcctttg ggaatcacgg ttacccttat ggctactact atggtggatg gcacgggcag
     1321 ccgatctacc gggatcgggg cgatcgggcg gcgtggcgca cgggcaccgg tgccgccgtc
     1381 gcctacggcg agggtattcg tgggcccgct gcgatgagta ctgctcagac cctatcacgc
     1441 ggcggctttg gaatgagttc ggccgcgcgc gctagctggg gcggctgaac catgcaacgg
     1501 gtatcggtgg ccgagcgctc agactggcgg aaacaggccg cgcagatggg gtttcacttc
     1561 cacacacttc agggtgtgcc ctactgggac gagtccgcct actatcgatt ttcgctcaac
     1621 gccgttgaaa acgacctgga gaaggtcacc gaagaagttc acgaactgtg catggatctg
     1681 gtcagccgcg cagtccggga cgaagtctat ctgcgaaggc tagcgattcc tgagcactat
     1741 tgggatttca tccgccactc ctaccgatcg ggtgaaccgc acatttatgg aaggatggat
     1801 ctggcctatg acggcaacgg gcccgcgaaa ctgtacgaac tgaactacga cacgccgacc
     1861 agcatctatg aggccggagt gtttcaatgg ttgtggctgc agcagtgttc ggagcgcggc
     1921 ctgattccgc gttccagtga ccagttcaat tcgttgcacg atcggctcca tgaggtgttc
     1981 cgcttcatcg ccgcgaaagt tcagtcgccg ctgtacttcg cctcggtacg tgactgcgtc
     2041 gaagatcgtg cgacggtgga ctatctgcgc gatgtggccc aacaggccgg cctcacgaca
     2101 cactatcttc cgatcgagga tatcggcgat accggggacg gcgcctacac cgatctggac
     2161 gatcagatca ttcgggccat cttcaagctg tatccatggg aatatatgtt tgcggatgag
     2221 tttggtcggt atctgccgac ggcgaatacc acttggtttg agccaccctg gaaatgcatt
     2281 ctttcaaaca aggccgccct ggcgcttcta tgggaactgc atcccggtca tcccaatctt
     2341 ctggcgacct acattgagca gggctcgcgc agtgatctgg cggcgggctg ggtgcgcaaa
     2401 cccttctatt cgcgcgaggg tgccaacgtt gaaatcgcaa cgccggccgg gcagcgattg
     2461 ctcaacgacg ggccctacac cgaaggcccg tttgtgcgcc aggcatttca tccgctgccg
     2521 cgctttggcg ataactatgc gctggtcggt agctgggtga tcggcgactg cgcggccggc
     2581 atcggaatcc gggaggccgc cgaactcatt acgaccgata cctccagatt cgtaccgcac
     2641 atcatagcgg actagcggat tcagcccgaa cgcgcgctca acgttatcga tcaatagtgc
     2701 cttgccgtcc caactcgcgc tgcatcgcct cttcgcgcgc atcgttgaca acccgcagca
     2761 cgctctcggg atccgcttgc gtctcatcgc gtgcgaactg cccggtgagc tttttgtctg
     2821 ccgtcagctc gccttgctcg tagacactcc acatctcgcg cgcgaactcg gtgcaaagca
     2881 gttcgggagc gaagcggcca agagtgccgc gaatgttgtt gacgtcgcgc tcgagcatgg
     2941 ccagcgcggc gttgttaccg gcggcgttca ccacctgcgg caggtcgatg atgacgggcc
     3001 cgtgtgagtc aaccagcaca ttgaactcgg acagatcacc gtggatgagt ccgatatgca
     3061 gcatgcgcac gatctgccgg atcagaaagg cgtgatactc acgagcctgc tcgcttgaca
     3121 gttcaacttc gcccagtcgc ggtgcggggt gcccgtcgga atcggtcacc agttccatga
     3181 ccagcacgtc gttgaaatag ccgtagggct tgggcacgcg cacgccggcc gcctgcaggc
     3241 gatacagcgc gtccacttcg gcgttcttcc aggccgtttc ctgttcgctg cggccgaagc
     3301 gcgtgcgctt gctcatggcg cgcgtttgcc ggctgccgcg cggcttgcgg ccttcctgat
     3361 attgcgcgcg gcgctggaag cttcgctgcc gcatgtcgcg atagaccttg gcacagcgcg
     3421 tctgtgcgcc gctgcgtacc aggtagaccg tggcttcttt gccgctcttg agtgcgcgaa
     3481 tgacttcgtc gatgacgccg tcgtcgatca gcggctgtag ggcagggggt gtcttaataa
     3541 agtgagatcc ggtggcgagg gtgcggcctt gacttggaat tgcacgagct tgaccgcgta
     3601 agcaactgat tatagccgat cagcgcggga aactcattca ggaactatca tgcccctgaa
     3661 gccctgcttg gcttgaaccc ccgcttctcg acattcattc gtgatagctt ggcgcacaac
     3721 caataggtag aagagccggc gggactgctc gtggaacaga gagttctcgc cgctctcggt
     3781 acgcagctcc atgaactcca agtgccgcgc tctgatgttc tgtctggctg cgctcgctgc
     3841 agcgagcgcg ccggtgcaca caagcgccgg cgacggattg gatgcattcg gcaccggcga
     3901 gcagcttcac agactgacgc caggtcttag cgatccgctg atgcagatgt gcgaggtacc
     3961 gggccgcccg ctgacgctgg caatgtcggt cgatctggct ctgtgccgca atccaaccac
     4021 acgtacggcg tgggcggccg cgcaccagca ggcagccttg ctcggcgagg cgaaaagtgc
     4081 ctggctgccc gatgcgacag cgaccggaac cgcgagccga accatcggac agcatctgga
     4141 gctgaccggt gagccgtcca cctctgatca gaacacagtg gatgcggcgg tcaacctctc
     4201 ctggaccctt tacgactttg gcgcgcgcac cgggcgtatc agcaacgcca ggtatctgct
     4261 cgatgcggcg gccgcgaccg ccggtagctc cgttcaacag accatcctga gcgtggtgca
     4321 gtcctactat ggcctcgcgg cggccgacgc cagtctggcg gcggccaaga ccacggagtc
     4381 ggtgacggcg catagcctgg aaatggcccg cactctgcgc agtgccggtc caggcacact
     4441 cgcggatgtc ctgcaggcgg aaaccgcata cgatcaggcg gtgctggcgc gcgaacaggc
     4501 ggatgccgcc gcgcaggccg cacgcgggac ctatgccgtg acgctcggat tgatcgccga
     4561 ttacggggta aagattgatg cggcaccggt tccggccgag gtgcccgctc agaaggcgcg
     4621 aatggcggac ctcatggccg aggcgatgcg tcagcgaccg gatcttgccg ccgcacagtc
     4681 gcaacgctcc gctgcggagg ccaatgtaca ggtggcgcgc gccgcggggt tacccgtgat
     4741 cacgatcaat gcgggcggcg attacatcac cagcaccggc attcccaatc agcattacgg
     4801 tctgatcggc atcaatgtgt cgatcccgct cttcaccggc ttcagtaccg cctatggtgt
     4861 acggcgcgcg aaggcagcgc tggaagagag tgaagtggcc ctggagcaga cacgactcgc
     4921 ggtttcactg ggtgtctgga acgcctacta cgcgcttgaa ttggcgaatg aacagctcgg
     4981 caccaccgct ggcctcgcca ggaccgcgga agataacgag cgcgtcgcct tgagcagcta
     5041 tcaggccggc gcaggcagat tcatcgacgt ggtgacggct caaaccgccg ccgccactgc
     5101 ccgtcaggtg cgtatcagtg cggagctgaa ctggcgcgtg tcgcgtgcgc agcttgcttt
     5161 ggccctgggg cgactgtcca gcgtcgagcc actgcaggag actcggacca ttccttgaag
     5221 tcacagcgcc tcgaaggccc gaagcacgcg cgcgaattcg tatggctgct ggacaatccc
     5281 gctgcagctc gggctcaccg tgacggccgg cggctcgcac gcgcgatcgc gattcagcga
     5341 ccatagcgat agcgagccca gatgctgctc ggtcgcgaac ttgagcagct gatcggcatc
     5401 gctcagctcg aacacctctg atgggatgtc attgtggcca atcatcggcg tgatgccgat
     5461 catttgccac agatcggtgc gagccatggt tgtcttgttt gcgggataga gcgcgcgcaa
     5521 ttgatcgcgc acggcgcgcg ccgccgtgat cgccaagctc cccattcggc ccgcgggctg
     5581 cggtgcggcg gcatcgccgt agtccatcgt catcgcattg attccgcgaa tcgccacgcg
     5641 ctgtccgatc gccgaacgaa tcacctccaa tccttggggc gtgagcccgg taggggtcac
     5701 cggcaacgtc aaccagactt ccagcggccg ctgctcggcc gccatcccct tccgcagcag
     5761 cgcgagcgct tcgctgcgac gctgaatcga ctggtgatcg gtaagcgctg tcccctccag
     5821 atcgaactcg atgacccgcg cgccataggc atcgatcacc gacctgtatc gcgtggccag
     5881 ctctgccccc gacttggccg cactcgccag ctcagtcccc gcctgacctc caaacgcgat
     5941 cgcgacatcc ccgccgagag tccgaagcgc gttgatttca cgcagacgaa aagacgaggt
     6001 cgcactgcgt gtgccgcccc aggtgggagc tggatcgtga gccgacctgg cgacgacaaa
     6061 gccgagtcgg aagtggcgca tgcgcagagc cttcgatgcg tcgagcaggc ttagttgagg
     6121 ccagagcgtc gcatcgacat agggtgcgaa ggtgcccggc ggccattcgg gcgcgctcgc
     6181 cgccctgacg ctggcggcgg gcggggccag cgacggttca ttcaggtgga tcggtgtg
//
LOCUS       DATMRM010000450         5258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4624787, whole genome shotgun sequence.
ACCESSION   DATMRM010000450 DATMRM010000000
VERSION     DATMRM010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4624787"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VNZ06_13615"
     CDS             <1..1097
                     /locus_tag="VNZ06_13615"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HWW21832.1"
                     /translation="LGTFLVRSGVLVSVHSFAADPSRGIFILAFLIAMIGGALVLYAW
                     RAPLMKSNAGFDFISRESFLLFNNILLAIACAVVFGGTLAPLIAETLKLGTLSVGPPY
                     FNPSFLVPVVPMLALVALGMHARWRRGGLGTARRAVAYALGVAVVLGLSLSLGVYTHP
                     LLLTPVGAIVGCWIILAALIDPIERLRQRQRIPSSVLGMSIAHIGLGVFALGITFVQS
                     NTIERDIALKPGQSTQLGDYLFRYQGGNIIDGPNYEGYRGRVDVERDGKPVTVLYPEK
                     RRYWVQGTVQTTAAIAPGLNRDLLASLGDDVGAGAWSLRLQYRPLIRLVWIGALIMAI
                     GGVVAVVGRRERAASPLRESLPAGTQGEGA"
     gene            1094..1651
                     /locus_tag="VNZ06_13620"
     CDS             1094..1651
                     /locus_tag="VNZ06_13620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HWW21833.1"
                     /translation="MTRFLLPLGAFALLVVVLIVGLRHAPEKNIIPSPLIGKSAPQFA
                     LQNLSDGHSPVSAALLRGHWSLVNVWGTWCPECRAEHEALLQIKREGQVPIIGIDWKD
                     ADDEAQAWLSQLGNPYSTVGTDHDGRVAIDWGVYAAPESFLINPDGVVVYKVIGAMTA
                     EIWHQKCLPLIQARSMPATQAGSGS"
     gene            1648..2100
                     /locus_tag="VNZ06_13625"
     CDS             1648..2100
                     /locus_tag="VNZ06_13625"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HWW21834.1"
                     /translation="MRGVRLAYALLGMSTLLAALPAMAVDNVPPLPTPQLQQRYYQLT
                     HELRCMQCQNEALADSPVGLAADLRLQVRELLIAGQTDEQIRQHMVDRYSEFILFRPR
                     MNWRNAWLWGAPGVLMLVGVVVAWRVIRSRSQLVDSEEADPNAESLNS"
     gene            2097..3080
                     /locus_tag="VNZ06_13630"
     CDS             2097..3080
                     /locus_tag="VNZ06_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21835.1"
                     /translation="MSLFVILAILLALLAALAVAWPLLRPRPSLPSAPIATMVTVLVV
                     LAGSALVYSRLGSPASARELPNADDTHSISALARHVESQPEDQAGWMALGAAYGSVGQ
                     FPLAIRAYQRANRLNDGGDANALLGIGEAMVLSGDASLGEKAPEYIERALQINPRSPK
                     ALFYGAVIAYHQERLPIARERFEALLALSPPQNVRVAVLKEIDDIDARLHPKIDESTA
                     IHLRVTLAAELASKVPPNALLFVFVPSPNGGPPLAVKRNAATLPQEVALSAADSMMAG
                     RAIQAGQKVSVVARISASGSPLPAKGDLYGQIDYVVGKSGERALQIDKLNP"
     gene            3103..4014
                     /locus_tag="VNZ06_13635"
     CDS             3103..4014
                     /locus_tag="VNZ06_13635"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HWW21836.1"
                     /translation="MTDGVLQRRLRAGEFAVTSEITPALSADPQTLLEKALPMRGLAD
                     AVNITDGASARAHLDPLTAAGILVANGVEPVLQLTCRDRNRIALQSMLVGAAALGVRN
                     LLALKGDDPKAGDQPDARPVFDLDSNALIVTARTIRDQGRLPHGRVVGGSAAFFIGAA
                     DAPIDPAPGWRPDSLLAKAQAGAQFIQTQFCMDIGVVRRYLARLCEFGVPQRLHILIG
                     LAPLASARSARWIRRHLFGSIIPDALIARLESASDERLEGQRICLELMQQLRQVPGVA
                     GVHLMAPLNETSLPAVIQQFRDGAARG"
     gene            complement(4011..4682)
                     /locus_tag="VNZ06_13640"
     CDS             complement(4011..4682)
                     /locus_tag="VNZ06_13640"
                     /inference="COORDINATES: protein motif:HMM:NF044762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWW21837.1"
                     /translation="MKVERASQHFAPAARRTDRRRVRTRAALLSAGRALLATRDIDGI
                     SVDEIVAAADVAKGSFYNHFSDKDVFAREIGGAVRRQVEQAVALANAGVGDPPLRVAR
                     GLCVYLRFAIEHRDSARVLWRLNSGSTMADAPINRELKRDLMQGMASRRFSSVDLEVA
                     LLLVMGAVVISMRHVLEERVSTPPFLVAANMAAGMLRSLGVARAEARRIAEGAAKGIF
                     SAVAR"
     gene            4753..>5258
                     /locus_tag="VNZ06_13645"
     CDS             4753..>5258
                     /locus_tag="VNZ06_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWW21838.1"
                     /translation="MQRSVTVAVSTHGDVALITVNNPPVNTINAAVRADLAAVLTQPS
                     VRNARALLLCCDGRTFFSGADIGEFSGPPKEAEYRALFATLEGMPVPVVAAMHGTALG
                     GGLEIALACHYRVAAPDARFGFPEVTLGIIPGAGGTQRMPRLIGAAGALELILSAKPM
                     GVPEAMQLG"
BASE COUNT          828 a         1669 c         1785 g          976 t
ORIGIN      
        1 tgctgggtac cttcctggtg cgttccggcg tactggtctc ggtgcattcg ttcgcggccg
       61 atccgtcgcg cggtatcttc attctcgcat tcctgatcgc gatgatcggc ggggcactgg
      121 tcctgtacgc ctggcgcgca cccctcatga agtccaacgc cggtttcgat ttcatctcgc
      181 gtgaatcgtt tctgctgttc aacaacatcc tgctcgcgat cgcctgcgca gtggtgttcg
      241 gcggaacgct ggcgccgctg atagcggaga cgctcaagct cgggacgctg tcggtcgggc
      301 cgccgtactt caatccgagt ttcctggtgc cggtggtgcc gatgctcgcg ctggtcgcgc
      361 tcggcatgca cgcgcgctgg cggcgcggtg ggctgggaac ggcccggcgt gcggtggcgt
      421 atgcactggg cgttgccgtg gtgctcggat tgtcgctgag cctgggcgtc tacacgcatc
      481 cgctgttgtt gacgccggtg ggcgcgatcg ttggctgctg gatcatcctg gccgcgctca
      541 tcgatccgat cgagcggcta cgccagcgcc agaggatccc gtcgagcgtg ctcggcatgt
      601 cgatcgcgca catcgggctc ggtgtgttcg cgctgggtat cacgttcgtg cagtccaaca
      661 ccatcgagcg tgatatcgcg ctcaaaccgg gtcagtccac gcagctcggt gactatctgt
      721 tccgctatca gggcggaaac atcatcgacg gcccgaacta cgaaggctat cgcggccgcg
      781 tggatgtgga acgcgatggc aagccggtca ccgtgctcta tccggagaag cgccgctact
      841 gggtgcaagg cacggtgcag accaccgctg cgattgcacc cgggttgaac cgcgatctgc
      901 tcgcatcgct cggcgatgac gtcggcgcgg gcgcctggag tctgcgcctg cagtatcgtc
      961 cgctcatccg gctggtgtgg atcggcgcgc tgatcatggc gatcggcggc gtggtggcgg
     1021 tcgtcggccg gcgcgagcgc gcggcctcgc cgctgcgcga atcgttgcct gcgggtacgc
     1081 agggcgaggg cgcatgaccc gattcctgtt gccgctcggg gcgttcgcac tgctggtggt
     1141 cgtgctgatc gtgggcttga ggcatgcgcc tgagaagaac atcattccgt caccgctgat
     1201 cgggaagagc gcgccgcagt tcgctctgca gaacctgtcg gatgggcaca gtccggtcag
     1261 cgccgctttg ctgcgcgggc actggtctct ggtcaatgtc tgggggactt ggtgcccgga
     1321 atgccgcgcc gagcatgagg cgttgctgca gatcaaacgc gagggtcagg tgccgatcat
     1381 cggcatcgac tggaaagatg ccgatgatga agcgcaggcc tggttgtcgc agctcggcaa
     1441 tccgtactct accgttggga cggatcatga cggtcgcgtc gcgatcgact ggggtgtcta
     1501 cgcggcaccg gagagcttcc tgatcaaccc cgacggcgtc gtggtctaca aggtcatcgg
     1561 cgcgatgacg gccgagatct ggcatcagaa atgtctgccg ctgattcagg cgcgcagcat
     1621 gccggcgacg caggcagggt ccggttcatg aggggcgtgc gactggcgta tgcgctgctt
     1681 ggtatgagca cgttgcttgc cgcgctgcct gcgatggccg tggacaacgt gccgccgctg
     1741 cccacgccgc agctgcagca gcgctactac cagctcaccc acgagctgcg ctgcatgcag
     1801 tgtcagaacg aggcgctcgc cgactcgccg gtggggctcg ctgccgatct acgcctgcag
     1861 gtgcgtgagc tgctgatcgc aggtcagacc gatgaacaga tccgccagca catggtggat
     1921 cgatacagcg aattcattct ctttcggccg cgcatgaact ggcgtaacgc ctggctctgg
     1981 ggcgcaccgg gcgtgttgat gctggttgga gtcgtggttg cctggcgggt gatccgctcg
     2041 cgctcgcaac tggtggatag cgaggaagcc gaccccaacg ccgagtccct gaattcatga
     2101 gtctgttcgt gatcctcgca atcctgctgg cgctgctggc ggcgctggcc gtggcctggc
     2161 cgctgctgcg gccgcgcccc tcgctgccgt ccgcccccat tgccacgatg gtgacggtac
     2221 tggtggtgct ggccggcagt gcgctggtct acagcagact cggctcgccg gcgagcgcgc
     2281 gcgagctgcc caatgcggat gacacgcact cgatctcggc gctggcgcgt cacgttgaaa
     2341 gtcagcccga ggatcaggct ggctggatgg cgctgggcgc ggcctacggc agcgtcgggc
     2401 agtttccgct ggcaattcgc gcttatcagc gtgccaaccg actgaacgac ggcggcgacg
     2461 ccaacgcgct gctgggcatc ggtgaggcga tggttctaag tggcgatgcc tcgctgggag
     2521 agaaagcgcc ggaatatatc gagcgcgcgc tgcagatcaa tccgcgctcg ccgaaggcgt
     2581 tgttctacgg cgcggtgatc gcctatcacc aggagcgcct gccgatcgcc cgcgagcgct
     2641 tcgaggcact gctggcgctg tcgccgccgc agaatgtgcg cgtcgcggta ctcaaagaga
     2701 tcgatgacat tgatgcacgc ctgcacccga agatcgatga atccacggcc atccatctgc
     2761 gcgtgacgct ggcggccgag cttgcctcca aggtgccccc caacgcgtta ctgttcgtat
     2821 tcgtgccgtc ccccaacggc gggccgcccc tggccgtgaa gcgcaatgcc gcgacgctgc
     2881 cgcaggaggt cgcgctgtca gcggccgatt cgatgatggc cggacgggcg atccaggccg
     2941 gtcagaaggt atcggtggtt gcacggattt cagcctccgg cagcccgttg ccagccaagg
     3001 gcgatctgta tggccagatc gactacgtgg tcgggaaaag cggcgagcgg gcgctgcaga
     3061 ttgataaact aaatccataa taagccaccc ggcctcgtaa gcatgacaga cggagttttg
     3121 caaaggcgac tgcgcgccgg tgagtttgcg gtcacctcgg aaatcactcc ggcgctgtcg
     3181 gccgatccgc agaccctgct cgagaaggca ctgcccatgc gcggtctggc cgacgcggtc
     3241 aatatcaccg atggtgccag cgcccgcgcc catctggatc ctctgaccgc cgccggtatc
     3301 ctggtggcca acggggtcga gccggtgctg cagctgacct gtcgcgatcg caatcgcatc
     3361 gcactgcaga gcatgctggt tggagcggca gcgctcggtg tgcgcaatct gctcgcgctc
     3421 aagggggatg atccgaaagc cggcgatcag cccgatgcca ggccggtgtt cgatctggat
     3481 tcgaacgcgc tgatcgtcac tgcgcgtacg attcgcgacc aggggcggtt gccgcatggg
     3541 cgcgtggtcg ggggcagtgc ggccttcttc attggtgcgg cggatgcgcc gatcgatccg
     3601 gcgccgggct ggcgaccgga ttcactgctg gccaaggcac aggccggagc gcagttcatc
     3661 cagacacagt tttgcatgga catcggcgtg gttcgccgct atctggcccg gctatgcgag
     3721 tttggagtgc cgcagcgcct gcatattctg atcgggttgg caccgctggc atcggcgcgc
     3781 tcggcgcgct ggattcgcag gcatctcttt ggctcgatca ttccggatgc actgatcgcg
     3841 cggctcgaga gcgcgagcga tgagcgactg gagggccagc gcatttgcct ggagctcatg
     3901 cagcagctgc gccaggtgcc gggagtggcc ggggtgcacc tgatggcgcc gctgaatgaa
     3961 accagtctgc cagccgtcat ccagcagttt cgcgacggcg cggcgcgcgg ctagcgcgcc
     4021 acggcgctga agatgccttt ggcggcaccc tcggcgatgc gacgcgcctc ggctcgcgca
     4081 accccgagcg agcgcagcat gccggccgcc atgttggcgg cgaccaggaa aggcggcgtg
     4141 ctgacgcgct cttcgagcac gtgccgcatt gaaatgacga ccgcccccat caccagcagc
     4201 aacgccacct ccagatccac cgaactgaag cgccgcgagg ccatgccctg catcaggtcg
     4261 cgtttgagtt cccgattgat cggcgcatcc gccatggtgg agcccgaatt cagccgccac
     4321 agcaccctgg cgctgtcgcg atgttcgatc gcgaagcgca gatagacgca cagtcctcgc
     4381 gccactcgca gcggcggatc gcctaccccc gcgttggcga gtgcgaccgc ctgttccacc
     4441 tggcgccgca ccgcgccgcc gatctcgcgc gcgaacacgt ccttgtcgct gaagtgattg
     4501 tagaagcttc ccttggcaac gtccgcagcc gcgacgatct catcgacact gatgccgtcg
     4561 atatcgcgcg tcgccagcag cgcgcggccg gcgctcagca gtgcggcgcg cgtacgtacc
     4621 cggcgccggt cggttcggcg ggccgcgggc gcgaagtgct gcgatgcgcg ttctactttc
     4681 atgtcaggcc gtatttgact atgtagttta aaaatgacta aatagtcaat aacattgtta
     4741 ccggggatcc gcatgcaacg cagcgtgacc gtagcggtga gcacccatgg cgacgtggcg
     4801 ctcatcacgg tcaacaatcc tccggtcaac acgatcaacg ccgcggtacg cgccgatctg
     4861 gccgcggtgc tcacccagcc ttcggtgcgc aacgcccggg cgctactgct gtgctgcgac
     4921 ggccgcacgt ttttctccgg cgctgacatc ggggaattca gcggcccacc gaaggaggcg
     4981 gagtatcgtg cgttgttcgc gacgctcgag ggcatgccgg tgccggtggt ggctgcgatg
     5041 cacggcaccg cgctgggagg cggcctcgag atcgcgctcg cctgtcacta tcgcgtcgcc
     5101 gctcccgacg cacgcttcgg atttccggaa gtgaccctcg gcatcattcc cggggcgggc
     5161 ggcacgcaac gcatgccgag gctgatcggg gcggccggcg cgcttgaact gatcctgtcg
     5221 gccaagccga tgggggtgcc cgaggcgatg cagctggg
//
LOCUS       DATMRM010000451        10121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1508525, whole genome shotgun sequence.
ACCESSION   DATMRM010000451 DATMRM010000000
VERSION     DATMRM010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508525"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VNZ06_13650"
     CDS             complement(<1..262)
                     /locus_tag="VNZ06_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21839.1"
                     /translation="MKIQPVPWLLMIAAGLHAVPAAAVVPDAQARVEQALHARLSLAY
                     PSVTRWEIEAMPAVRRAARTMTATGPQVQQIVITRIGARSAVW"
     gene            complement(267..1049)
                     /locus_tag="VNZ06_13655"
     CDS             complement(267..1049)
                     /locus_tag="VNZ06_13655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HWW21840.1"
                     /translation="MFDSLYIGATGMVAHESDVDTIANNLANLNTVGFRRGVVSFSEV
                     SAQLAISALDPSGVASAPATVPGAGSVAQTSLSTLAGPLQTTNQPLDLAIDGSGFLEV
                     LRADGTLACSRAGSLQVNPDGMLAIADGTPLSPQIQIPPDAQSIAISPTGQVTALVGS
                     STTPVLLGRIGLVNFANPAALTIVGNNQYSAPPAAGNPQTGAPGSAGFGLLRQGFLEA
                     SNVQMTQEMVSLMLAQRGFELNSKMVQTADQLWSITNGLVRS"
     gene            complement(1059..1772)
                     /locus_tag="VNZ06_13660"
     CDS             complement(1059..1772)
                     /locus_tag="VNZ06_13660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HWW21841.1"
                     /translation="MANVMGILASTMRTDAEALRVIAQNVSSADNVAYRRQILVSRPD
                     FDSLAHSAQAQLLLPKVQTAIDSRPGTLKSTGESLDLAIQGDAYFVVSTAQGELLTRR
                     GDFHVDPTGVLSAFNGDPVLGTEGTIRMPEGTPKIDLDGTVRIGSETVGHLRLEELAP
                     EASVRPMGDALFASQGGAPVASPSAQVHQGFLETSNVSPVNEMLHLLQAVRHFEAGQR
                     FVRTYDEMLDRALSDLGKI"
     gene            complement(1772..1969)
                     /locus_tag="VNZ06_13665"
     CDS             complement(1772..1969)
                     /locus_tag="VNZ06_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21842.1"
                     /translation="MSTQHPQLLSAPHDPRQLERLYRVAADQPVEPDLMRVMASLLIH
                     VLGVDEASQPNPPEPFTQEKD"
     gene            complement(1966..3234)
                     /locus_tag="VNZ06_13670"
     CDS             complement(1966..3234)
                     /locus_tag="VNZ06_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HWW21843.1"
                     /translation="MSVPHIASHASGHSPTQSRPLPLMRELSPRWEIGQAVSVKVQQP
                     LHPPFYLAQIAGGPSQFVESAVALSAGANVRAVVVAVGARLELKLVDGTHDASIVVSG
                     SDPGAAGLSELEASAPLVASAMAPGSSDSIAAQVRTLSAQYRLTLGERDQAQLITAVS
                     RAKEQPAMLLAGLYLASIDVPLTRNALEALYLAQTRQPLRRLASDAATALNLTALTGA
                     SAAPAQAALEAVVGRMDRVINGEATGQLTQMSVAAPEVATAGAPSDSGAPGDRWSDAS
                     GDHVAELAMQLLNVSDRPAVARHFGTFPVLVSGQLLELQFVAIRHDDARCEQPASRRL
                     LMALTTPSLGRIQVSAQAQGKRLILQIAGHSTRATEILAQHAGEVRELAMRLGWQVDS
                     VLYRVDPQPGGAASEAMELALREGDMDQLL"
     gene            complement(3231..5276)
                     /gene="flhA"
                     /locus_tag="VNZ06_13675"
     CDS             complement(3231..5276)
                     /gene="flhA"
                     /locus_tag="VNZ06_13675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HWW21844.1"
                     /translation="MMGWLRQVFAEQSELVLALMLGGVLLVLFAPIPPWLLDFLLITN
                     FAGALLILLLTFYTARPLEFSTFPSLLLVATLFRLGLNVAATRLILSQANAGHVIAAV
                     GRYAVGGNYVIGLIVFIVLIVVQYVVVTNGAQRVAEVAARFTLDAMPGKQMSIDADLN
                     MGLIDQTEARERRRGVEREANFYGAMDGASRFVKGDAIAGILIILVDIIGGLAIGVAQ
                     NGMSWSESLQTYTLLTVGDGIVTQIPALVIATGTGIIVTRAASDARLGAEVSRQILGH
                     PRTIAIVAVVLLALGLLPGIPVVPTLVLGTLMAILFWVARRHGKSAAVAQPPPPSSAA
                     DKAEDLYKTLKVDPVEIVISTTLMSLVGEEGRILADKLTGIRKQYAADMGVVLPLVRV
                     RDERRQTSNRYEVCVFGARVAEGEVVPERLLAINPGGTRPPLEGQVALDPAYRLPAVW
                     IEEHQRVAARAAGYTVVDALTVFMTHVAEVLKQNAHNLITRAETERLIARVREQQSSL
                     IDELIPKVLSLGDVQRVLQGLVRERVPIRNIDAILEVLTDHGSKVKEADSLTELVRER
                     LGRMICQQLVDSHGELQVLTFDPAVEQSLAAAVRQVDDKPTLIVEPRMAEQVLRKLSE
                     EVERMARGNLRPVLLCAPTLRRHVRRLTERLVPQLAVLSLNEVPTQLNLRAFGTVKV"
     gene            complement(5273..6346)
                     /locus_tag="VNZ06_13680"
     CDS             complement(5273..6346)
                     /locus_tag="VNZ06_13680"
                     /inference="COORDINATES: protein motif:HMM:NF013479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HWW21845.1"
                     /translation="MQQQEQERTEQATPFKLEESRDRGEIQRSGDFTTFALLAAMVAM
                     LVGWGAASWGHLQRTSVEWLSSASGSSELLVPLVLLGRDLLQVVVPFGLVGMALAILG
                     NILQSGPVFSFAPVMPRFARINPISGFRRLFSKRIIFETFKSLLKLGLLSGVAYGFFR
                     ALWPQATEPGQSTPAGVLEFIGRHSVALLVRFAAVLLIIGLIDWAFVRWRYRQQMMMS
                     RREVKEEIKRREGDPQIRSRLRELQRENLKQSRALGRLPDADVLITNPDHVGVALCYV
                     RGEMTAPCVIAKGTGLWLVQMKALAWRHSVPVRQQPPLARRLFRYGRIDQPIPSDTYQ
                     DVARLYADLAAERRRTARYEVHT"
     gene            complement(6347..7114)
                     /locus_tag="VNZ06_13685"
     CDS             complement(6347..7114)
                     /locus_tag="VNZ06_13685"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HWW21846.1"
                     /translation="MTVSFNLGWLVATALLSIRVATATALTAVFGPTQVPGAVRVLVA
                     SMLAGLMVSVGGFAAPQIETMGQLLVAGVGEALIGAALALGFLAAYAATQVAGRVLDT
                     QMGFGIASIFNPATRDVSPLLGTVLGMAAISIFLAIDGHHQIIRALALSIKSYPPGSS
                     LSAMNAGALLLQSGLMFSFALALAAPTMCMLLMADIAMAVFARSMPQLNIFVMGFAIK
                     IMLGLIVLIGSIRLSLPVFNALFRATFNYWSRIAVGA"
     gene            complement(7126..7392)
                     /locus_tag="VNZ06_13690"
     CDS             complement(7126..7392)
                     /locus_tag="VNZ06_13690"
                     /inference="COORDINATES: protein motif:HMM:NF013480.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HWW21847.1"
                     /translation="MSPDFIVGLISQLLKTAALLIGPLIGVTTLVGLVTSVLQGVTQI
                     QESSLTFVPKLIAAGAVLLVMGGWMLKVLTEYASTLINNIPNYF"
     gene            complement(7819..8673)
                     /locus_tag="VNZ06_13695"
     CDS             complement(7819..8673)
                     /locus_tag="VNZ06_13695"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWW21848.1"
                     /translation="MPHRAQRNPDSASGDWYVDNRCIDCAAAREVAPGLIIHRGGKSV
                     FARQPATPEEEAAAWRAALLCPTASIGTAAGEHPQPPHLFPQPLAPGVYRCGYNARSS
                     FGAHSYLARRAAGNVLIDSPRFTRPLVDAIREWGGLAEVLLTHRDDVADAGRYAQEFG
                     ARVWIHEEDAAAAPYATNLLRGYEATSLGADLIAIAVPGHTRGSVAYLWQDEYLFTGD
                     SLAWSHATHDLAAFREVCWYSWAELRKSLSQLTNYAFSWVLPGHGHSVHLPRDDMQAR
                     LRRLAESM"
     gene            8759..9625
                     /locus_tag="VNZ06_13700"
     CDS             8759..9625
                     /locus_tag="VNZ06_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21849.1"
                     /translation="MSSGIQRLSQSGAVAALLTLGGCGADSMNMNMDMPAASSTSSST
                     SMTLMATFDSIQANVFTPICASCHGGANPAANLALDAMHSYNDLVNVPSSEQPTIVRV
                     KPFDPNNSYLIIHLQKQGDGAPSVDVPVIAQWISDGAMPGMSMMGGGMGMSMQASAHF
                     QVSATQPNSGDVMTAPPPRVIIGFTQELDASSVNPGSVRLERVDPVPESSGSMTTVPT
                     VMSVTPGNARALILTPSSALSPGNYQVVLESSVGSDLRSQLGAALSAPAPQSSGLRVV
                     ARFSVAPPAAAE"
     gene            9769..>10121
                     /locus_tag="VNZ06_13705"
     CDS             9769..>10121
                     /locus_tag="VNZ06_13705"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HWW21850.1"
                     /translation="MTDPKLTKLELQVMEALWSGSPRSIRNIQQTFPARKRPAYTTIQ
                     TIVYRLEGKKAVRRVRKVGNFHVFEAAVTRDIAQGRLIDELLALFGGRSQPVMARLIE
                     SGKLTLADIKEAEKTL"
BASE COUNT         1965 a         3360 c         3026 g         1770 t
ORIGIN      
        1 cccaaacggc gctacgcgcg ccgatccgtg tgatcacaat ctgctgtacc tgcggtccgg
       61 ttgccgtcat cgtgcgcgcc gcacgccgca ccgcaggcat cgcttcgatc tcccagcgcg
      121 tcacggacgg gtaggcgaga ctcagcctgg cgtgcagggc ttgctcgaca cgggcctgcg
      181 catcggggac taccgccgcc gcaggcactg cgtgcagccc cgctgcgatc atcaaaagcc
      241 acggcaccgg ctgaatcttc atgcgactac gaacgcacca gtccgttggt gatcgaccag
      301 agctgatcgg cggtctgcac catcttgctg ttgagctcga agccgcgctg agccagcatc
      361 agcgacacca tctcctgcgt catctgtacg ttcgacgctt cgagaaatcc ctgacgcagc
      421 agaccgaaac cggcggaccc tggcgcgcca gtctgtggat tgcctgcggc gggcggcgcg
      481 ctgtattgat tgttgccgac aatggtcagc gcggcgggat tggcgaaatt gaccaagccg
      541 attcgtccca gcagcaccgg cgtcgtcgaa ctacccacca gcgccgtcac ctgcccggtg
      601 ggcgagatcg cgatgctttg tgcgtccggc gggatctgaa tctgtgggga cagcggcgtg
      661 ccgtcggcga tcgcgagcat gccatccgga ttgacctgca gcgagccggc gcgcgaacac
      721 gccagcgtgc catccgcgcg cagtacttcg aggaaaccgg atccgtcgat tgccagatcc
      781 agcggctggt tggtggtctg aagcggcccc gccagggtag acaggctggt ctgcgccacc
      841 gagcccgcgc ccgggacagt cgccggcgcg ctggcaacgc cgcttggatc cagcgcgctg
      901 atggccagct gcgcactgac ttcggaaaac gagacgacgc cgcgccggaa accgaccgta
      961 ttcagattgg ccagattgtt ggcaatcgtg tccacgtcgc tctcatgtgc caccatgccg
     1021 gtggcgccga tataaagtga atcaaacatg ttcttctcct agatctttcc cagatcactc
     1081 aaagcgcgat cgagcatttc gtcataggta cgcacgaagc gctgtccggc ctcgaagtga
     1141 cgtaccgcct gaagcagatg cagcatttca ttcaccggcg agacgttgga ggtctcgagg
     1201 aatccctgat gaacctgggc actggggctg gcgaccggcg cgccgccctg acttgcgaac
     1261 aacgcgtcgc ccatcggccg cacgctcgcc tccggcgcca gctcttcgag ccgcaggtgg
     1321 ccgaccgtct cgctgccgat ccgaacggtc ccatccagat caatcttcgg cgtgccctcc
     1381 ggcattcgaa tcgtgccctc ggtcccgagc accggatcac cattgaaagc actgagcact
     1441 ccggtcgggt ccacgtgaaa atccccgcgg cgcgtgagca gctcgccttg cgcggtactc
     1501 acaacaaaat aggcatcgcc ctgaatcgcg aggtcgagcg attctccggt gctcttcagc
     1561 gtccctgggc ggctgtcgat tgccgtctgc accttcggca gcagcagttg cgcctgggcc
     1621 gagtgcgcga gactgtcgaa gtcgggcctg ctcaccagta tctggcgtcg gtaggccacg
     1681 ttgtcggcgc tggaaacatt ctgcgcgatc acgcgcagcg cctcggcgtc ggtgcgcatc
     1741 gtgctcgcaa gaatgcccat cacattcgcc atcagtcttt ctcctgcgtg aacggttccg
     1801 gcggattggg ctgtgaggcc tcatcgaccc ccagcacatg gatcaacagc gaagccatga
     1861 ccctcatcag gtcaggttcg accggctgat ccgcggccac tcgatacagt cgctcgagct
     1921 gcctggggtc gtgaggcgct gacagcaact gcgggtgctg cgtactcata gcagctgatc
     1981 catgtcgccc tcgcgcaggg ccagttccat ggcctcactg gcggcgccac ccggttgggg
     2041 atctacacga tagagcaccg aatccacctg ccagcccagg cgcatggcga gctcgcggac
     2101 ttcgccggcg tgttgcgcca ggatttcggt ggcacgcgtt gaatgcccgg cgatctgaag
     2161 gatcaggcgc ttgccctggg cctgagcact cacctggatg cggcccaggg acggggtcgt
     2221 cagcgccatc agcaaacggc gcgaggcggg ctgctcgcaa cgcgcgtcat cgtgccggat
     2281 ggcgacgaac tgcagctcca ggagctgtcc ggacacgagc accggaaaag tgccgaagtg
     2341 acgcgcaacc gcaggccgat ccgaaacatt caatagctgc attgcaagct cggcgacgtg
     2401 atcacccgac gcatcgctcc accgatcgcc cggtgcgcct gagtccgacg gcgcaccggc
     2461 ggtagcgacc tccggcgctg ccaccgacat ctgcgtcaac tggccggtcg cctcgccatt
     2521 gatcacccga tccatgcgtc cgaccaccgc ctcgagtgcg gcttgcgccg gcgcggcact
     2581 cgcacccgtc agcgcggtca gattgagcgc cgtggcagca tcactcgcga gccgcctcag
     2641 gggctgacgg gtctgcgcca gatacaacgc ttccagtgcg ttgcgagtca gcggcacgtc
     2701 gatcgacgcc agataaagac ccgccagcag catcgcgggc tgctctttgg cgcgactcac
     2761 cgcggtgatc agctgcgcct gatcgcgttc accgagtgtc aaccgatatt gagcgctcag
     2821 tgtcctgacc tgtgcggcaa tgctgtcgct ggagcccggc gccatggcgc ttgcaacgag
     2881 gggcgcactc gcttcgagct ctgacagtcc ggcggcgccg gggtccgatc cgctcaccac
     2941 aatgctcgcg tcatgggtcc cgtccacgag cttgagctcc aggcgcgcgc cgaccgccac
     3001 cacgacggcg cgtacgttcg ctccggcgct cagcgcaacg gcgctttcaa cgaactgcga
     3061 cgggccaccg gcgatctgcg ccaggtagaa cggcggatgc agcggttgct gcaccttgac
     3121 gctgaccgcc tgtccgatct cccaccgcgg cgatagctcg cgcatcaatg gcaacggccg
     3181 cgattgcgtc ggagagtgcc cggaggcatg actggcaata tggggcacgc tcatactttc
     3241 accgtgccaa atgcccgaag gttcagctgg gttgggacct cattgagcga gagcaccgcc
     3301 agctggggca ccagtcgttc ggtcaggcgt cgtacatgcc gacgcaaggt cggcgcacac
     3361 agcagcacgg gtcgcaggtt cccgcgcgcc atgcgctcga cctcttccga cagcttgcgc
     3421 agaacctgct ccgccatgcg cggctcgacg atgagcgtcg gcttgtcatc cacctgacga
     3481 acggctgccg ccagcgactg ttcgacagca ggatcgaagg tcaggacttg gagttcgccg
     3541 tgtgaatcga ccagttgttg acagatcatc cgtccgaggc gctcgcgcac caactcggtc
     3601 agcgagtctg cctccttgac cttcgagccg tgatcggtga gtacctcgag gatcgcgtcg
     3661 atattgcgga tggggacgcg ttccctgacc aggccctgca acacccgctg cacatccccc
     3721 aacgagagca ccttgggaat cagctcatcg atcaggctgg attgctgctc gcgtacccgc
     3781 gcgatcagcc gctcggtctc agctcgggtg atgaggttgt gcgcgttttg cttgagcacc
     3841 tcggcgacgt gggtcatgaa aaccgtcaag gcatcgacga ccgtatagcc agcggcgcgc
     3901 gccgccaccc gctgatgctc ctcgatccat acggcaggca gccgataggc cggatccaac
     3961 gcgacctgac cttcaagcgg tggccgcgtg ccaccggggt tgatcgcgag cagccgctcg
     4021 ggaacgacct caccctcggc caccctcgcc ccaaagacgc agacttcata gcgattggag
     4081 gtttgacgcc gctcgtcacg cactcgaacc agcggcaaca ccacacccat gtccgccgcg
     4141 tactgtttgc gaattccggt aagcttgtca gcgaggatgc ggccctcctc tcccaccagc
     4201 gacatcaatg tcgtgctgat gacgatctcc accggatcca ctttgagggt tttgtacaga
     4261 tcttcggcct tgtcggcggc gctactcggc ggcggcggct gagccacggc ggcactcttg
     4321 ccatgccggc gcgcaaccca gaaaagaatt gccatcagcg tcccgagtac gagcgtcggc
     4381 acgacgggga ttcccggcag cagtcccagc gccaacaaca ccaccgcgac aatcgcgatg
     4441 gtgcgcggat gacccagaat ctgacgtgag acttccgcac ccaggcgcgc atcggaggcg
     4501 gctcgcgtga cgatgatgcc ggtcccggtc gcaatcacga gcgccgggat ctgtgtcacg
     4561 atgccatcgc ccacggtcag cagcgtatag gtctgcagcg actcggacca gctcatgcca
     4621 ttttgcgcca cgccaatcgc aagcccgccg atgatatcca ccagaatgat cagaatgccg
     4681 gcgatcgcat cccctttgac gaagcggctg gctccgtcca ttgcgccata gaaattcgcc
     4741 tcgcgctcga cccctcgacg ccgttcacgc gcttcggttt ggtcgatgag acccatgttc
     4801 aggtcggcat cgatgctcat ctgcttgccg ggcatcgcgt ccagtgtgaa tctggcggcg
     4861 acttcggcga ctcgctgtgc accgttggtg acgaccacgt actgcacgac gatcagcacg
     4921 atgaacacaa tcagtccgat gacgtagttg ccgcccacag cgtagcgacc gaccgcggcg
     4981 atcacatggc cggcattggc ctgcgacagg atcaaacgcg tcgccgccac attcagtccc
     5041 aaccgaaaca gcgtcgccac cagcagcagt gaggggaacg tggagaactc gagcggccgg
     5101 gccgtgtaga acgtcagcag cagaatcaac aaggcgccgg cgaagttggt gatgagcagg
     5161 aagtccagca gccatggtgg gataggcgca aacagtacaa gtagaactcc gccgagcatt
     5221 agcgccagca ccagctcact ttgctcggcg aacacctggc gcaaccagcc catcatgtgt
     5281 gcacctcata gcgtgcggtg cgtctgcgct cggcggcgag gtcggcgtac agccgcgcca
     5341 cgtcctggta ggtatcggat gggatcggtt gatcgatgcg gccgtaccga aacaggcggc
     5401 gcgcgagcgg tggctgctgt cggaccggga ccgagtggcg ccaggccagc gctttcatct
     5461 gcaccagcca cagcccggtt cctttggcga tcacgcacgg cgccgtcatc tcgccgcgca
     5521 catagcacag cgccactccc acatgatccg gattggtgat cagcacatcc gcatccggca
     5581 gacggcccag cgcacgcgac tgcttcagat tctcccgttg cagttcgcgc aagcggctcc
     5641 tgatttgtgg atcaccctcg cgccgtttga tctcttcctt gacttcacga cggctcatca
     5701 tcatctgttg acgatagcgc catcgcacaa aggcccaatc tatgagtcct atgatcagca
     5761 ataccgcggc gaagcgaacc agcagcgcta cgctatgcct gccaataaac tccaggactc
     5821 cggccggcgt gctttgtcct ggttctgtcg cctgcggcca cagcgcccgg aagaacccgt
     5881 aggccactcc agagagcaga ccgagcttca gaaggctctt gaacgtctcg aagatgatcc
     5941 gcttgctgaa gagacgtcga aagccgctga tcggattgat gcgcgcaaag cgcggcatga
     6001 cgggcgcgaa gctgaacacc ggcccggatt gcagaatatt tcccaggatg gccagtgcca
     6061 taccgaccag gccgaatgga accacgacct gcagcagatc acggcctagt agcaccagtg
     6121 gcacgagcag ctcagagctg ccgcttgccg aggacagcca ctccacgctc gtgcgttgaa
     6181 ggtgccccca cgaggccgct ccccacccca ccaacatggc gaccatggct gccagcaatg
     6241 caaatgtagt gaagtcaccg ctgcgctgga tctcgccgcg gtctctggat tcctccagtt
     6301 tgaacggggt ggcttgttcc gttctttcct gttcctgttg ctgcatctag gcgccgaccg
     6361 caatacggct ccagtagttg aaggtcgcgc ggaacaaggc attgaacacc ggcagcgaca
     6421 gccggatcga cccgatcaga acgatgagac cgagcatgat cttgatcgca aagcccatga
     6481 caaagatatt cagctgcggc atgctgcgcg cgaataccgc catggcgatg tccgccatga
     6541 gtagcatgca catggttggc gccgccagcg ccagcgcaaa gctgaacatc aggccagact
     6601 gcagcagcag cgctcccgca ttcatcgcgc tcaacgagga gcctggcgga tagctcttga
     6661 tcgacagcgc gagcgcgcga atgatctgat gatggccgtc aatggccaga aagatgctga
     6721 tggcagccat tccgagcacc gtacccagca acggcgaaac atcacgcgtt gccggattga
     6781 agatgctggc gataccaaat cccatctgtg tgtcgagcac gcgcccggcc acttgcgttg
     6841 ccgcataggc cgccagaaat ccgagcgcca gtgcggcccc aatgagtgcc tcgccgacac
     6901 cggccacgag cagctgcccc attgtttcaa tctgcggcgc cgcgaagccg ccgaccgaga
     6961 ccatcagtcc cgccagcatc gacgccacga gtacccgcac cgctccgggc acttgagtcg
     7021 gaccgaatac cgccgtcagc gcggtcgcgg tcgcgacgcg tatcgaaagc agtgcggtcg
     7081 caacgagcca acccagattg aacgagactg tcacaggttt cgtggctaga agtagttggg
     7141 aatgttattg atgagcgtcg aggcgtattc ggtcaggacc ttcagcatcc aaccacccat
     7201 gaccagcagt accgcgccgg ccgcgatcag tttcggaacg aacgtcagcg aggactcctg
     7261 gatctgcgtc actccctgca gcacgctggt taccagtcca acgagagtcg tcacaccaat
     7321 caatgggcca atgaggagcg cggccgtctt cagcagttgc gagatcaaac ccacgatgaa
     7381 atccgggctc atatcaccga ccacgaccga tcccctggcg tgagacgcct agtcttcgtt
     7441 tcaatgcaac gacaagaatt tgcaagcctc gccatcgcgg tgcttctcat cgcacctgaa
     7501 ttagacggcg acccgaaccc tcaactgtac gacacagcaa tccttagctg tcagaccctg
     7561 actcctggtt tctgatgtta tttctttgtc atctgtccta tcgatctcgt gacatcagat
     7621 ttatcacatt tgaatgcaat ccggcaatcg cgaaccgctg cctggggatc attaatgcga
     7681 ccgacggctt atcgatgcct cgaagccgac gcgacggcaa gcgctactgt taagtccgag
     7741 tggcgcggaa cctacagaat gtgcaccgat acgccgccgc gcgggatgcg cgatacggca
     7801 cagtccgggc gtcgagcgtc acatgctctc ggccagtcgt cgcagccgtg cctgcatatc
     7861 gtcgcgcggc agatgaacgc tgtgcccgtg cccaggcaac acccagctga atgcatagtt
     7921 cgtcagctgg gagagcgact tcctgagttc agcccacgag taccagcaca cctcgcggaa
     7981 ggcggccaga tcgtgcgtgg catgactcca cgccaatgaa tcgcccgtga acagatactc
     8041 gtcctgccac agataggcga cgctgccgcg tgtgtgtccg ggcaccgcga tcgcgatcag
     8101 atccgcaccg agggaggttg cctcgtatcc gcgcagcagg ttcgtggcgt acggtgcggc
     8161 cgctgcatcc tcttcgtgaa tccacacccg cgcaccgaac tcctgcgcgt aacgcccggc
     8221 gtctgccaca tcatcgcgat gcgtcagcag cacctccgcc agtccgcccc actcccgaat
     8281 cgcgtccacc agaggccggg tgaagcgcgg cgagtcgatc agaacgttgc cggccgcacg
     8341 ccgcgccaga taggagtgcg cgccgaatga ggaacgggcg ttatacccgc agcgatacac
     8401 gccgggcgcg agcggctgag gaaacagatg cggtggctgg gggtgttcgc ccgcagccgt
     8461 accgatggag gcggtcggac acaacagcgc cgcgcgccag gcggcggcct cctcttccgg
     8521 cgttgcgggt tgccgcgcaa acaccgattt gccaccgcga tgaatgatca accccggtgc
     8581 gacttcccgc gccgcggcgc agtcgatgca acggttgtcg acgtaccagt cgccggaggc
     8641 cgaatccgga ttgcgttgcg cgcggtgtgg cataggattt cgctatttta aaccgccaag
     8701 ccatccgcgg gcactcacag agcgtagaac cagaagtgca gcgcaggtct tactctgcat
     8761 gtcatcaggc atccaacgac tctcccagag cggcgcagtg gccgcactgt tgacgctggg
     8821 cggctgcggc gctgattcga tgaacatgaa tatggacatg cccgcggcct ccagcacgag
     8881 cagttccacc tccatgacac tgatggcgac gttcgattcg atccaggcca atgtttttac
     8941 gccgatttgc gccagttgcc acggcggcgc caatccggcg gccaatctcg cccttgacgc
     9001 gatgcacagc tacaacgacc tggtcaacgt cccgagcagc gagcagccca cgatcgtccg
     9061 cgtcaagccg ttcgatccga acaacagcta cctgatcatt catctgcaga aacaaggcga
     9121 tggcgcgcca tccgtcgacg tcccggtcat cgcacagtgg atctcggacg gtgcgatgcc
     9181 aggcatgtcg atgatgggcg gcggcatggg aatgagcatg caggccagcg cccattttca
     9241 ggtatccgcg acgcagccca actccggcga cgtcatgaca gcgcctccgc cacgggtgat
     9301 catcggattc acgcaggaac tggatgcaag cagcgtgaac ccgggttcgg tcagactcga
     9361 gcgcgtggat ccggtgccgg agagctccgg ctcgatgacg accgttccga cggtgatgtc
     9421 ggtgacgccg ggcaacgccc gggccctgat actcacgccc tcctcggcac tgtcacctgg
     9481 caactatcag gtggtgctgg aatcctccgt cggcagtgac ctgcgttcac agttgggcgc
     9541 ggcactgagc gcaccggccc ctcagagcag cggcctgcgc gtggtcgcac gcttcagcgt
     9601 cgcgcctccc gccgccgccg agtaataccc ctctaccccc cgccgcaggt gtcccgcgat
     9661 ggcccagtgc cgtcgcggcg cgcctgcttt cccggccgcc tttctatcac ggttgcactt
     9721 tcgcacatcg tcatagtagc atcacctaca tctgtagtag gtactactat gaccgaccca
     9781 aagctcacga aactggaatt gcaggtgatg gaagcgctct ggagcggcag cccgcgctcg
     9841 atccgcaaca ttcagcagac ctttccggcc cgcaagcgcc ccgcctacac gacgatccag
     9901 accatcgtct accgcctgga gggcaagaaa gccgtgcgac gcgtgcgcaa ggtcgggaat
     9961 ttccacgtat ttgaggcggc agtgacgcgc gacatcgccc agggtcggct catcgacgag
    10021 ctgctggcgc tgtttggcgg gcgcagccag ccggtcatgg cccggctcat cgaatccgga
    10081 aagctcacgc tcgctgatat caaagaggcg gaaaaaaccc t
//
LOCUS       DATMRM010000452         6137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2116392, whole genome shotgun sequence.
ACCESSION   DATMRM010000452 DATMRM010000000
VERSION     DATMRM010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6137
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2116392"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..1299
                     /gene="purD"
                     /locus_tag="VNZ06_13710"
     CDS             13..1299
                     /gene="purD"
                     /locus_tag="VNZ06_13710"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HWW21851.1"
                     /translation="MKVLIVGGGGREHALAWKCAQSSRVDEVIVAPGNAGTQCEPRVR
                     NVDIAACDTARLIELAQREAVQLTIVGPEAPLVAGIVDAFQAQGLRCFGPSEHAARLE
                     GSKAFAKDFLQRHGIPTAAYRSFTQNNYDPAWLRAQHAPIVIKASGLASGKGVIIAQT
                     LDEAEAHVQAMFHGRFGESGAEVVVEEFMHGEEASFIVMADGEHVLPLASSQDHKRLY
                     DGDLGPNTGGMGAYSPAPVVTRAIHARVMREVIGPAIRGLKQEGNPFTGFLYAGLMIG
                     QDGTVRVVEFNCRLGDPETQPILARLRSDLPALCEAALDGRLDSMQAKWDPRPALGVV
                     LAAAGYPEKVRGGDAIQGLEAATRCAGKMFHGGTRRVEGDFVTSGGRVLCAVGLGKRV
                     SEAQRNAYALVDCIRYEGMQYRRDIGARAIAREQDS"
     gene            1296..2528
                     /locus_tag="VNZ06_13715"
     CDS             1296..2528
                     /locus_tag="VNZ06_13715"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW21852.1"
                     /translation="MKPVAFAAFQQRGFRMFFVGNATTMMADNVEHVISYWVMFQKFH
                     SPELGGFAVISHWVPFLLFSGYSGALAARLDPRRMIQLGILLFMSVSLCWGVLIATGT
                     LQIWHAAVLLSIHGCAGVLWGPPSQLLIHDIVGKQALPSAVRLTATSRYVGMLLGPAV
                     GNALMLLLGPSHGIFANAAIYLPMLLWLWRAPYGPRFRRGDAPRLGVGGLADAIATLR
                     SIRGNNTILSMTMLAGAVSFFVGNAYQAQMPNFATDLGHGDAGVSYAALTAADAAGAL
                     GGAIMLEARGLLAPRVGTAFILSMLWCIALSGFALTHSYPLALGLLFCAGFLELAMSA
                     MTQALVQLNAPPQLRGHVIGVYAMAASGLRFVSGMTVGLLGGLLGVHASLSSAAGALL
                     VVIALLFIWQRVREVREV"
     gene            complement(2532..3359)
                     /locus_tag="VNZ06_13720"
     CDS             complement(2532..3359)
                     /locus_tag="VNZ06_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21853.1"
                     /translation="MRSWWFGAAMLAVGVSAHSQEYLSPPPEAASPITDHLAFRAIYF
                     LGKVTTHAQFDPSANINNGVGTNFTAEQLLGLSEKADQFRVELVFRLEDRNRLRVNFL
                     DLRRAGDRELTQQLQFGNTTFNVGEAVQSEFDYREFDLTYTYSFLKGRWYELGAGLGV
                     QLLEAEADADVAATPRFAKFSGAGPFATPALDGTIVFDRHWSLNARAQYLHIAVKSAG
                     GLWEDYHGDLQYRWRRNLAAGLGFEYQEIGLNLNHSNPAGLVRLKLVGPEAFLRASF"
     gene            complement(3452..4708)
                     /gene="rho"
                     /locus_tag="VNZ06_13725"
     CDS             complement(3452..4708)
                     /gene="rho"
                     /locus_tag="VNZ06_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWW21854.1"
                     /translation="MNLTELKAKPIHELVKMAESMALEGMARSRKQDIIFSILKAHAR
                     NGEDIYGDGVLEILQDGFGFLRSADGSYLAGPDDIYVSPSQIRRFNLRTGDTISGLIR
                     PPKDGERYFALLKVGEINFDSPDSSRNKVLFENLTPFHPTRRMKLERGNGSTEDLTAR
                     TIDLVAPIGKGQRGLIVSPPKAGKTMLLQNIATSITANHPEVYLIVLLIDERPEEVTE
                     MQRTVKGEVVSSTFDEPAARHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTILATALIDTGSKMDDVIYEE
                     FKGTGNSEIHLDRRISEKRVFPAVNINRSGTRKEELITDPGELAKMWILRKLLHPMDE
                     LAAIEFLLDKMKDTKANAEFFEAMKR"
     gene            complement(5011..5337)
                     /gene="trxA"
                     /locus_tag="VNZ06_13730"
                     /pseudo
     CDS             complement(5011..5337)
                     /gene="trxA"
                     /locus_tag="VNZ06_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
     gene            5627..>6137
                     /locus_tag="VNZ06_13735"
     CDS             5627..>6137
                     /locus_tag="VNZ06_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWW21855.1"
                     /translation="MSDHHLSSMTFGSLPLAPELMQGIVDAGFTYCTPIQAQTLPSAL
                     QGRDIAGQAQTGTGKTAAFLVALYQSLLSKPPTPTRSPTSVRALIVAPTRELAVQIHK
                     DAALLGKHTPLKLAVVFGGTDYEKQRRSLTDGVDILVGTPGRIIDYFKQGVFDLRHAH
                     VLVLDEADRM"
BASE COUNT         1076 a         1863 c         1954 g         1244 t
ORIGIN      
        1 cactagcgct cgatgaaagt cctgatcgta ggtggcggcg gccgcgagca cgcactggcc
       61 tggaagtgcg cgcagtcgtc gcgagtcgac gaagtcatcg tcgctccggg caacgccggt
      121 acgcagtgcg aaccgcgggt acgcaatgta gacatagcgg cttgcgacac cgcgcgactg
      181 attgaactgg cgcagcgcga ggcggtgcaa ctgaccattg tcgggccgga ggcaccactg
      241 gtcgctggca tcgtcgacgc gttccaggca caggggctgc gctgcttcgg acccagtgaa
      301 cacgccgcgc ggctcgaagg ctcgaaagcc tttgccaagg atttcctgca acgccacggc
      361 ataccgaccg cggcctatcg cagcttcacg cagaacaact acgaccctgc ctggctacga
      421 gcgcagcacg cgccgatcgt catcaaggcc agcggactgg cctcgggcaa gggcgtcatc
      481 atcgcgcaga cgctggatga ggccgaagcc cacgtgcagg ccatgttcca cggtcgcttc
      541 ggtgaatcgg gcgccgaggt cgtcgttgaa gaattcatgc acggtgaaga agccagtttc
      601 atcgtgatgg ccgatggcga gcatgtgctg ccgctggcca gctcgcagga tcacaaacgc
      661 ctttatgacg gggacctggg accgaacacc ggcggcatgg gagcctattc gccggcaccg
      721 gtggtcacgc gtgcaatcca cgcgcgcgtc atgcgcgagg tgatcggacc cgcgatccgc
      781 ggcctgaagc aggaaggcaa tccgttcacc ggcttcctgt acgcagggct gatgatcggc
      841 caggatggta cggtacgggt ggtcgagttc aactgtcggc tgggcgatcc ggagacgcag
      901 ccgatcctgg cgcgactgcg cagcgatctg cctgcgctgt gcgaggcagc gctcgacggc
      961 aggctcgata gcatgcaggc aaagtgggat ccccgcccgg cgctgggcgt ggtactggcg
     1021 gcggcgggtt atccggagaa ggttcgcggc ggcgacgcca tccaggggct ggaggccgcg
     1081 acccggtgcg ccggcaaaat gtttcacggc ggcacccgcc gcgtcgaagg cgacttcgtc
     1141 accagtggcg ggcgggtgct gtgtgcggtc ggcctgggta agcgggtcag cgaagcccag
     1201 cgcaacgcct atgcgctggt ggactgcatc cgctacgagg gcatgcagta ccggcgcgac
     1261 atcggcgcgc gcgcgatcgc tcgcgaacag gattcgtgaa accagtcgcg ttcgccgcgt
     1321 ttcagcaacg cggatttcgc atgttcttcg tcggcaacgc caccacgatg atggccgaca
     1381 acgtggaaca cgtcatcagc tactgggtga tgttccagaa gtttcactcg cccgaactgg
     1441 gcggcttcgc ggtgatttcg cattgggtgc cgttcctgtt gttctccggt tactccggtg
     1501 ccctcgccgc tcgactcgat ccacgtcgca tgatccagct gggaatcctg ctattcatgt
     1561 cggtatcgct gtgctggggc gtgttgattg cgaccggcac gctgcagatc tggcacgccg
     1621 cggtgctgct gagcatccac ggctgcgcgg gggtactgtg gggtcctccc tcgcagctgc
     1681 tgatccatga catcgtcggc aagcaggcgc ttcccagcgc cgtacgcctg accgcgactt
     1741 cacgctacgt cggcatgctg ctggggcctg cggttggcaa cgctctgatg ttgctgctcg
     1801 gtccatcgca cggcattttc gccaacgcgg ccatttatct tccgatgttg ctgtggctgt
     1861 ggcgtgcacc gtacggtccg cgctttcggc gcggcgatgc tccgcgctta ggcgtaggcg
     1921 ggctggccga tgcgatcgcc acgctgcgaa gtattcgcgg caacaacacc attctgtcga
     1981 tgacgatgct ggccggcgcg gtatcgttct ttgtcggcaa cgcctatcag gcgcagatgc
     2041 cgaactttgc gaccgattta ggccacggcg atgccggcgt ttcctatgcc gccctgaccg
     2101 cggcggacgc agcaggtgcg ctgggagggg cgatcatgct ggaagcgcgc ggcctgctcg
     2161 cgccgcgcgt ggggaccgcg ttcatcttga gcatgctgtg gtgtatcgcg ctgagcggtt
     2221 tcgcgctcac gcattcctac ccgctggccc tgggcttgct gttttgcgcc gggttcctcg
     2281 agcttgcgat gagcgccatg actcaggcgc tggtacagct gaacgcgccg ccgcaattgc
     2341 gcggtcacgt gatcggcgtg tacgcgatgg ctgcctcagg actgcgcttc gtgagcggca
     2401 tgacggtggg cctgctgggt gggctgttgg gtgtgcacgc ctcgctgtcg agcgcggccg
     2461 gtgcgctgtt ggttgtcatc gcgctgctgt tcatttggca gcgcgtccgc gaagtccgtg
     2521 aagtctaaag gctagaaact cgcccgcagg aatgcttccg gaccgacgag cttgaggcgt
     2581 accaagcccg ccggattgga gtgattgaga ttcaggccga tttcctggta ttcgaagccg
     2641 aggcccgcgg ccagattgcg tcgccaacgg tattgcaggt cgccgtggta gtcctcccac
     2701 agaccgcctg ccgacttgac cgcgatatgc aggtactgcg cccgcgcatt cagcgaccag
     2761 tggcgatcga acacaatcgt gccgtccaga gccggtgtgg caaatgggcc ggcacccgaa
     2821 aacttcgcga aacgcggggt cgccgcgacg tcggcatccg cttctgcctc aagtaactgc
     2881 actccgagac cagcgcccag ctcataccag cggcccttca ggaacgagta ggtataggtc
     2941 agatcgaact cgcgatagtc gaactcggac tgaaccgcct ctcccacgtt gaatgtggtg
     3001 ttgccgaact gcagctgctg agtcagctcg cgatcgccgg cgcggcgcag gtccaggaag
     3061 ttcacccgca ggcgattgcg gtcttccagc cggaagacca gctcgacacg aaactggtcg
     3121 gctttttcgc tcagacccag cagctgctcg gccgtaaaat tcgtgccgac cccgttgttg
     3181 atgttggcgc tcggatcgaa ctgcgcgtgg gtcgtgacct tgcccagaaa gtagatcgcc
     3241 cggaacgcga gatgatcggt gatcgggctc gccgcctcag ggggcggact caaatactcc
     3301 tgcgagtgcg ctgagacacc caccgcgagc attgccgcac cgaaccacca tgaacgcatg
     3361 cttgagccga ctcctgcaac ctcagaagga tttcagccta agggcgcgac cgctcaccgt
     3421 gacgcaccac tcagtttgcg acgagccgct cctaccgttt catcgcctcg aagaactcgg
     3481 cgttcgcttt cgtatccttc atcttgtcga gcaggaactc gatggcggcc agttcgtcca
     3541 tggggtgcag cagtttgcgc aggatccaca tcttcgccag ctcacccggg tcggtgatca
     3601 gctcttcctt gcgcgtgcct gaacggttga tattgaccgc gggaaataca cgtttttcgg
     3661 aaatccggcg atccaggtga atttcgctgt tgccggtacc tttgaactct tcgtagatga
     3721 catcgtccat cttcgagcca gtatcgatca gcgcggtggc caggatcgtc agactcccgc
     3781 cctcctcgat gttgcgcgcg gcgccgaaga agcgtttcgg tcgttgcagc gcattggcat
     3841 cgacgccgcc ggtgagcacc ttgcccgatg agggcacaac ggtgttgtaa gcacgcgcca
     3901 gacgggtgat cgagtcgagc aggatgacca catccttctt gtgttcgacc agacgcttgg
     3961 ccttctcaat caccatctcc gcgacctgca cgtggcgcgc cgccggctcg tcgaaggtac
     4021 tggaaaccac ttcgcctttg accgtgcgct gcatctcggt cacttcctcc ggccgctcgt
     4081 cgatgagcag cacgatcagg tagacctcgg gatgattggc agtgatcgag gtggcaatgt
     4141 tctgcagcag catcgtcttg ccggccttcg gcggcgagac gatcaggccg cgctgaccct
     4201 tgccgatcgg ggcgaccagg tcgatcgtgc gcgcggtgag gtcctcggtg ctgccgttgc
     4261 cgcgctcgag tttcatgcgg cgggtcggat ggaatggggt caggttctcg aacagcacct
     4321 tgttgcgcga gctgtccgga gagtcgaaat tgatctcgcc gaccttcagc agcgcgaaat
     4381 aacgctcacc atccttcggc ggtcggatca gtccggaaat cgtatctccg gtgcgcagat
     4441 tgaagcgtcg gatctggctc gggctcacat agatgtcgtc cggacctgcc aggtacgaac
     4501 catccgcgga acgcaggaag ccgaagccgt cctgcagaat ctcgagcacg ccatcgccgt
     4561 agatgtcctc gccattgcgt gcgtgggcct tgagaatcga aaagatgatg tcctgcttgc
     4621 gcgaacgggc catcccctcg agcgccatcg actcggccat cttgaccagc tcgtgaatcg
     4681 gcttggcctt gagctcggtc aggttcatcg agcggcgcga agcggccaga tcggctggac
     4741 tgattatctc ggcatcatct tccccgatcg gttccagatc gggcagcatc tgatcgtcgg
     4801 gacggccgtt accgcggttg ccgtcgcggt ttccgtggcg cgggcctttg ttgttgttag
     4861 gtcgattgcg accctgattg ccgaggtagc ctctcatact ttaccggtcg tacgcgccgc
     4921 gggcgcaggt ggaatggtgc cggtggtacg cgccgtgggc gcggccgcaa tggtgccggt
     4981 cgtgcgcgta gcgtgtgctg ccggaatcgg caggtgctga tccaggaagg tcgccagctg
     5041 cgatttcgac agcgcaccca ccttttgcgc ctcgaccgtg ccgtccttga acaggatcag
     5101 ggtcggaatg ccgcgaatgc cgtatttcgg cggggtctgc gggttctcgt caatgttgag
     5161 cttggcgatc ttgagccggt ctttgtactc ggtggcgatc tcgtccagga tcggcgcgat
     5221 catcttgcat gggccacacc actcagccca aaagtccagc aacacaggcc tgtcggcctt
     5281 gaggacgtcc tgatcgaagc tgccgtcaga agtatgcaca atgtgctcgt tactcacgta
     5341 gttgagcctc cgttgaatgg gagttctttg agattgggaa cagaaaagat ttaaaaaggt
     5401 ggattcttag caaataaccg aactgaattt gtcgcggcag agcagctgtc gtgccccggg
     5461 aagccgtcta acggttacac ttggatctgg gttagccctg agatgcttgc gtcaggttgg
     5521 cttggcaggt gcggacagaa gagccgctgg ttgaggttat ttttagccct ttcgggggta
     5581 ctttgcaagc cttaggttac gacgtcgcca attacaaaca gcttgaatga gtgatcatca
     5641 tctcagttcc atgaccttcg gttcgctgcc tctggcaccc gaactgatgc agggcatcgt
     5701 tgatgccggt ttcacctatt gcacgcctat ccaggcacaa acgttgccga gtgcactgca
     5761 gggccgcgat attgccgggc aggcgcagac cggcacgggc aaaaccgcgg cctttctggt
     5821 tgcgctgtat caatcgctat tgtcaaagcc gccaaccccc acccgcagtc cgacctcggt
     5881 tcgtgcgctg atcgtcgcgc ccacgcgcga gctggcggtg caaatccaca aggatgcggc
     5941 gcttctcggc aagcacacac cactcaaact cgcggtggta tttggcggta ccgactacga
     6001 gaagcagcgg cgcagcttga cggacggggt cgatatcctg gtcggcactc ccggtcgcat
     6061 catcgactat ttcaagcagg gcgtattcga cctgcgccac gcccatgtac tggtactcga
     6121 tgaggccgat cgcatgt
//
LOCUS       DATMRM010000453         6469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4301019, whole genome shotgun sequence.
ACCESSION   DATMRM010000453 DATMRM010000000
VERSION     DATMRM010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4301019"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VNZ06_13740"
     CDS             complement(<1..811)
                     /locus_tag="VNZ06_13740"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWW21856.1"
                     /translation="MRNDSASTFRPDIEGLRGIAVFVVVAFHCGLPGFSGGFIGVDVF
                     FVLSGYLITGLLLQELETTSTIGLLNFYARRVRRLLPASVLMLLVTLAVGALVLAPQE
                     LDFAARAARATAVYLGNVFFAQQARDYFAADVRTNPLLHMWSLAVEEQFYLFWPLLML
                     LLWRRAKSRATLAAAFALLTVVSLIACVWLTVHSRTVAFYALYTRAWEFGIGGLASLV
                     PRGRSGAPASVWGLAGWLGVIAIGLSSALLTPAAAFPGWLATIPVVGTALAL"
     gene            1104..2282
                     /locus_tag="VNZ06_13745"
     CDS             1104..2282
                     /locus_tag="VNZ06_13745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW21857.1"
                     /translation="MAKGINILFLIDWFHRTGGTEKHLVQLIAGLPADRFRCTVVAFD
                     MGVNPLLDELRARDVPVIHLPVGREYVPNAAVQAWRLSRLIRRNRYDIVQTFHQKADT
                     FGALIVWLSGARRLVSSKRDTGDLRKPLHVFLNRRLRSLFQAFIVVAEAVRVAVVARD
                     RLPDARVTTIYNGVDLSKFTPPTAQQRVEARTRFGFGSGDFVAGMVAGFRPEKSHDVF
                     FAGLAQALSSIPTLKVLAVGGGELLSQFQEQISHGMLAQRVVFTGELTDVLPCLWSMD
                     VACLTPGSNEGFSNALIEQMAVGLPLIVTDVGGNAEAVIDGVNGYVVPVGDAGALSAA
                     LIRMYQNREAQRAMGRASRQRAEERFSLHRMCAEHARLYQALCPAGQLVKAEQVDREY
                     "
     gene            2320..3348
                     /locus_tag="VNZ06_13750"
     CDS             2320..3348
                     /locus_tag="VNZ06_13750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWW21858.1"
                     /translation="MGFSVAVTSSQGFEREILRLRNANRDRPETSQYINWRYQHLAGA
                     PPPQVFWLLAPQGERIGMASVVFRPYWLNGTRVQTAVVGDISLDARWRGRGLGRLLLQ
                     HMTAYLDEHFPQYPGFVIPTEAARHALAKAGWSAPGTLVPHVYLVDVTRYVRAIVRST
                     WLTAVIAPPWRWGARAFARLRRPRAGELILSDTPGEAVHEFLRSVAPSEGAMHDLGAQ
                     TLRWRYQQHPNTPFVFATYTRGGEVRGVLVFEETSLEGICHVYDLAARAPADMRAMLA
                     LFLLRSSDTPGIASVRMLTDDRHPARSTLRVLGFSPRRAEAVYQVHSRDGSAERYTWR
                     VTQGDKDT"
     gene            3380..4471
                     /locus_tag="VNZ06_13755"
     CDS             3380..4471
                     /locus_tag="VNZ06_13755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWW21859.1"
                     /translation="MSALRVRRWTNEEWLANQASWAALQARSSADRLFLSWEWLTLWW
                     RCYGSTLGRTPDILAFYRGADLVGLAPLYHRRVLRGGVLLAHSVQVIGLSWRDPEPLI
                     SEYLDVIAAREDLDEVRRACAQVLLEQPSWSELVIGFTAAADCWRDAFAPPVSSTQRY
                     VRNTDRCVSYQADLSEGFGPYLRTLGQSTRRSVWNLRRRLASLGEVRVEQLRDDEIEE
                     GFADLNRLHALRWRRPAFTGKRLSFHTSLARLLVARGELAFSRLSIGGEVVSVLYDIR
                     REQRQYNLKMGFDPAFSVRVSLGLIHLGYAMEAAAAEQISVYDFLAGPGRTSDYKRHL
                     SQVRRDLSCVQILRGRFLPSLYRWRDRAR"
     gene            complement(4468..5406)
                     /locus_tag="VNZ06_13760"
     CDS             complement(4468..5406)
                     /locus_tag="VNZ06_13760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW21860.1"
                     /translation="MSDSVRVSVVMPLYNAEKYVKSALGSALASDLRELEVIVVDDGS
                     RDASAAIVAAIDDPRVTLLRTTPPSGGPSRPRNTGIARARAPYIALLDADDLLKPHKL
                     SAAIAALERHPQAGFVFGDFERIDAQGALLEASTLAGYPVFQSLAAVELQPPWRLIPQ
                     AELARGLLYENFIGTSGVVMRKELLDEIGGFDETLVCSEDRDLWFRAAHHGAALYCNE
                     VGHSYRVIPSSLSHAPVIRNARCRIAVLQREKARWTDAAARAQVKQLITENLANIAYA
                     YWRSRRRFAAVLLFLQVFARSGQLRWLRGAFGSLLP"
     gene            complement(5471..>6469)
                     /locus_tag="VNZ06_13765"
     CDS             complement(5471..>6469)
                     /locus_tag="VNZ06_13765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW21861.1"
                     /translation="RVGKGIYEFYEQAIARPLNNPQAVGVVRMALESQPDLILAHRLS
                     SMSVFMRLAAELNGRPLFFDLDDIEHVAFARRLLHHPGYPRERLMLLQTPRLWAAELQ
                     ALRLATSTFVCSEPDRRYLSRFVGAARVQTVPNSARFPSLNEEDMSNPTILFIGSMAN
                     QPNAQAVDVLVQRIWPKVRAEVADARLVIIGSGAEATRAYPAKDPSVSFAGFVGDLAP
                     WYRDARVVCCPIYHGSGTRVKIIEAAAHAKAIVSTRLGAEGLEFEDGREIMLRDTPQA
                     LADACVQLLQSASSARRLGLAARARAAELYEHGAIVRRLERIFASAGDSARVPEPR"
BASE COUNT         1195 a         2003 c         2103 g         1168 t
ORIGIN      
        1 tgagcgcgag cgcggtcccg actaccggga tcgttgcgag ccatcccggg aaggccgctg
       61 cgggtgtcaa gagcgcgctc gagagcccga tggcgatgac gcccaaccag ccggccaggc
      121 cccagacgct ggcgggcgct ccggagcggc cgcgcgggac gagactcgcg agacctccaa
      181 ttccaaattc ccaggcgcgc gtatacaagg catagaaggc aaccgtgcgg ctgtgtaccg
      241 tcagccacac gcacgcgatc agtgatacga ccgtgagcag cgcaaaagct gcggcgagtg
      301 ttgcgcgtga ttttgcacgg cgccacagca gcagcatcag cagcggccag aacagataga
      361 actgctcctc cactgcgagt gaccacatgt gtaacagcgg attcgtccgc acatcagcgg
      421 cgaaatagtc gcgtgcctgc tgcgcaaaga agacgttgcc gaggtacacg gcggtggcac
      481 gggcggcgcg cgcggcaaag tccagctcct gtggcgcgag cactaaggcg cccaccgcca
      541 gggtcaccag aagcatcagc acactcgccg gaaggagtcg gcgcacccgg cgggcgtaga
      601 agttcaacag cccaatggtg gaggtcgtct cgagttcctg cagcaacaac ccggtaatga
      661 gataacccga caggacgaaa aagacatcga ccccgatgaa gcctccggag aagcctggca
      721 atccgcagtg aaacgcgacc accacaaaga ccgcgatgcc gcgcagtccc tcgatatcag
      781 gtcgaaacgt gcttgccgag tcgttgcgca tcaaaccttg ctgagccttt cctttagcgg
      841 cggcgagcgc gcaatccgcc gcatgcgcca ggcaaagacg cggacgattg cgatcaagaa
      901 tagcgcccct cggcggatgt caccgtgcac cctgtcccgg cgccaagcgg acacatgggc
      961 ccaggcaccg agtccgccct ggcgccgtaa ggcattgatg taactaacaa ttgttgagat
     1021 tgcagagtga gtgcggcggc gcgggggcag ccgcaggaga tggatatact ggccgcgcgg
     1081 gttggcggtc cggcgatgta tgaatggcta agggcatcaa tattctcttt ctgatcgact
     1141 ggtttcaccg cacgggcggt accgaaaaac atctcgtgca actgatcgca gggctgcctg
     1201 cggatcgctt tcgctgcacc gtcgtcgcct tcgacatggg ggtgaatccg ctgctggacg
     1261 aactgcgggc ccgcgatgtt ccagtcattc acctgccggt aggacgcgag tacgtgccca
     1321 acgcggcagt gcaggcgtgg cgtttgtcac ggctgatacg gcgcaacaga tatgacattg
     1381 tgcagacctt tcaccagaaa gccgacacct tcggtgcgct catcgtgtgg ctctccggcg
     1441 ccaggcgctt ggtatcgagc aagcgcgaca ccggtgatct gcgcaaaccg ctgcatgttt
     1501 ttctcaaccg tcgcctcaga tcgctctttc aggcgttcat tgtagtagca gaggccgtac
     1561 gcgtggccgt ggtggcgcgg gatcgactgc cagacgcacg cgtaaccacc atctacaatg
     1621 gcgttgacct cagtaaattc actcccccta ctgcgcagca acgggtcgag gcacgcacgc
     1681 gctttggttt tggctcaggg gatttcgtgg cgggtatggt agcgggcttc cggccggaaa
     1741 agagccatga cgtgtttttc gcagggttgg cgcaagcgct atcgagcatt cccaccttga
     1801 aggtcttagc ggtggggggc ggggaactgc tgagccagtt ccaggagcag atctcgcacg
     1861 gcatgcttgc acagcgcgtg gtgtttaccg gcgagctcac cgacgtattg ccctgtcttt
     1921 ggagcatgga cgtcgcctgc ttgacgcccg gtagcaacga ggggttctcc aacgcgctga
     1981 tcgaacagat ggccgtgggt ttgccgctga tcgtcactga cgtcggcggt aacgccgaag
     2041 cggtcatcga cggtgtcaac ggttacgtcg ttccggtggg tgacgcaggt gcactatctg
     2101 ccgcgctgat caggatgtat cagaatcgcg aagcccagcg cgccatggga cgcgcctcgc
     2161 gccagcgtgc agaggagcga ttttcgctgc atcgcatgtg cgccgagcac gcgcgactct
     2221 accaagcgct ttgtccggct gggcaacttg tgaaggccga gcaggtagat cgtgaatact
     2281 gatccgagtg ggtcgcgcga ttcggatagg cgcgcgaccg tgggattttc cgttgcggta
     2341 acctcctcgc aggggttcga gcgggaaata ctgcgcctgc gaaatgccaa ccgcgaccga
     2401 cccgaaacct cgcagtacat caactggcgt tatcagcacc tggcaggtgc accgcccccg
     2461 caggtgttct ggttgctcgc gccgcagggt gagcgcatcg gtatggcttc ggtcgtgttt
     2521 cgaccatact ggttgaatgg cacacgggta cagaccgccg tcgtcggtga catttcactc
     2581 gatgcacggt ggcgcggacg gggcctgggc cgcttgctgc tgcagcacat gacggcctac
     2641 ctcgacgaac acttcccgca ataccccggc tttgtgattc ccaccgaggc agcacgccac
     2701 gcgctggcca aggccggctg gagcgcgccc ggcacgctcg tgccacacgt ctacctggtc
     2761 gatgtcacgc gctacgtgcg agcgatcgtg cgcagcacct ggctgaccgc cgtgatcgcg
     2821 cccccgtggc gctggggcgc gcgtgccttt gcgcgcctgc ggaggccgcg cgcgggtgaa
     2881 ctcattctga gcgatacgcc cggtgaggct gtgcacgaat tcctgcgcag cgttgccccg
     2941 agcgagggcg cgatgcacga cctcggcgcc cagacgctca gatggcgcta ccagcagcac
     3001 ccgaacactc cttttgtttt cgccacctac acgcgcggcg gtgaagtgcg cggtgttctg
     3061 gtcttcgagg agacctcgct cgagggaatc tgccacgtct acgacctggc cgcgcgcgca
     3121 ccggcagaca tgcgcgcgat gttggcgctt ttcctgctgc gcagttcgga cacgcccggg
     3181 atcgcaagcg tgcgcatgct gaccgatgat cgtcaccccg cgcgcagcac tttgcgagtc
     3241 ctcgggttca gccctcgtcg tgcggaagcg gtgtatcagg tgcattcgcg cgatggcagc
     3301 gccgagcggt acacctggcg cgtgacccag ggcgacaagg acacctgatg gcacgccatg
     3361 cgtcagcgag gaggggacga tgagcgcgct gcgggtgcgc cggtggacga acgaggagtg
     3421 gcttgcgaat caagcgtcgt gggctgcgct gcaggcgcgc tcgagcgcgg atcggctgtt
     3481 cctttcctgg gagtggctga ccctctggtg gcgctgctac gggagcacgc tgggtcgcac
     3541 gccggatatc ttggcgttct accgcggtgc ggatctggtg ggtcttgccc ccctgtatca
     3601 ccgccgcgtc ctgcgcggtg gagtgctgct ggcgcactcg gtgcaggtca tcgggttgtc
     3661 gtggcgcgat ccggaaccgc tgatttccga gtacctggat gtcattgccg cgcgcgagga
     3721 cctggatgag gtgcgccgcg cgtgcgcgca ggtgttgctc gaacagccct cgtggagcga
     3781 actcgtcatc ggctttactg cggctgccga ttgctggcgt gatgcgtttg cgccaccggt
     3841 gtcgagcacg cagcgctatg tgcgcaacac agatcgctgc gtcagctacc aggcggacct
     3901 tagcgagggc tttggcccat acctgcgcac gctcggacag tcgacgcgtc gcagtgtctg
     3961 gaacttgcgc cgtcgcctcg ccagcctcgg cgaggtgcgt gtcgagcaac tgcgtgacga
     4021 tgagatcgag gagggtttcg cagatctgaa taggctccac gctctgcgct ggcggcggcc
     4081 ggcgttcacc ggcaagcgac tgtcatttca tacgagcctg gcgaggctgc tggtggcgcg
     4141 cggggagctg gcgttttcgc gcctgagtat tggcggtgaa gttgtttcgg tgctctatga
     4201 catccgcaga gagcagcggc aatacaatct gaagatgggc tttgatcccg cgttcagtgt
     4261 gcgggtatcg ctcggactca ttcacctggg ctacgccatg gaggcggccg cggccgagca
     4321 gattagtgtc tacgactttc ttgccggacc tggtcgcacc agcgactaca agcgccatct
     4381 gagccaggta cgacgcgacc tgagctgtgt ccagatccta agaggccgtt tcctgccttc
     4441 gctatatcgc tggcgggacc gggcgcgcta aggcaacagc gagccgaatg ctccgcgcaa
     4501 ccagcgcagc tggcctgaac gagcaaacac ctgcagaaaa agcagcaccg ctgcgaagcg
     4561 gcgtcgcgac cgccagtatg cgtaggctat gttggcgagg ttttcagtaa tcagctgttt
     4621 gacctgagcg cgcgcggccg cgtctgtcca acgggctttc tcccgctgca ggacggcgat
     4681 gcggcaacgc gcgttacgga ttaccggggc gtggctgagg ctgctgggga ttacgcggta
     4741 ggaatgacca acctcgttac agtacagcgc cgcgccgtgg tgggccgccc gaaaccacag
     4801 atcacgatct tccgagcaga cgagagtttc gtcgaagccg ccgatctcat ccagtagctc
     4861 tttgcgcatc acgacgccag aggtgccgat gaagttctcg tagagcagcc cccgggcaag
     4921 ttcggcctgc ggtatgaggc gccacggagg ctgcagctcg acggccgcga gcgattggaa
     4981 caccgggtat cccgcgagcg tggatgcttc aagcaacgcg ccttgagcgt cgatgcgctc
     5041 gaagtcgcca aagacgaacc cggcctgcgg atgccgctcg agcgcggcga ttgccgccga
     5101 gagcttgtgc ggcttgagca gatcatcggc atccagcagc gcgatataag gcgcccgcgc
     5161 ccgggcaatt ccggtattgc gtgggcgcga agggccacca ctgggcggcg tggtccgcag
     5221 caacgtgacg cgcggatcat ctatagcagc aacaatggcg gcagaggcgt cgcgcgagcc
     5281 gtcgtccaca acaattacct cgagctcgcg cagatcagac gcaagcgcgg agccgagggc
     5341 ggactttacg tacttctcgg cgttgtaaag tggcatcacc acgcttacgc gcacgctatc
     5401 agacacgtac gagtcccttg gccttcaggg tgtcctgcac acgagcactg acagccacgg
     5461 cgttaccacc tcaacgcggt tccggcacac gtgccgaatc accggcgctc gcaaaaatgc
     5521 gctccagtcg cctcacgatc gcgccgtgct cgtacagctc cgcggcgcgt gcgcgcgctg
     5581 cgagccccaa gcgccgagcg ctgcttgcgc tctgcagcaa ctgaacgcag gcgtcagcaa
     5641 gcgcttgcgg agtgtcgcgc agcatgatct cgcgaccgtc ctcaaactcc aatccttcgg
     5701 cgccgagccg tgttgacacg attgccttgg cgtgcgctgc tgcttcgatg atcttcaccc
     5761 gtgtgccgga gccatgatag atggggcaac atacgacgcg cgcatctcgg taccagggcg
     5821 cgaggtcccc gacgaaaccg gcaaagctca cactcggatc ctttgcaggg taagcgcgcg
     5881 tcgcttctgc accgcttccg atgatcacca ggcgcgcgtc ggccacctcg gcgcgtacct
     5941 tgggccaaat ccgctgcacc aacacatcga ccgcttgcgc attgggctgg ttcgccatgc
     6001 tgccgataaa taatatggtc gggttcgaca tgtcctcctc gttcagactg gggaacctgg
     6061 cactgttggg gacagtctgt acgcgcgcgg cgcccacaaa tcgcgacaga tagcggcgat
     6121 cgggctcgga gcacacgaaa gtggatgtcg caagccgcag cgcctgcagt tctgccgccc
     6181 agagccgcgg cgtctgcaag agcatcaggc gctcacgcgg atagcccggg tggtgcaaca
     6241 ggcgccgtgc gaaggccacg tgctcgatat catcgaggtc gaagaagagg ggacgtccgt
     6301 tcagctcggc cgcgagtctc atgaagacac tcatcgagga taagcggtgt gccagaatca
     6361 gatccggctg actttcaagg gccatccgca cgaccccgac ggcttggggg ttgttcagtg
     6421 gccgagcgat agcctgctcg taaaactcat agattccttt cccgactcg
//
LOCUS       DATMRM010000454         6902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_591773, whole genome shotgun sequence.
ACCESSION   DATMRM010000454 DATMRM010000000
VERSION     DATMRM010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591773"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VNZ06_13770"
     CDS             complement(<1..398)
                     /locus_tag="VNZ06_13770"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWW21862.1"
                     /translation="MKLVNFGPEASADSLRIVDGPTPVPGDGQILIEVKFAGVNRPDV
                     LQRMGRYNPPQDASPILGLEVSGRVAALGPNVSQWRVGDQVGALVHGGGYAQFCVACA
                     SHALPVPNGLSLEQAAAFPENWFTVWANLI"
     gene            545..988
                     /locus_tag="VNZ06_13775"
     CDS             545..988
                     /locus_tag="VNZ06_13775"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HWW21863.1"
                     /translation="MASKKELSAAAADTLLKVDGLSQSDVERLVTASYRVRGYEVRAV
                     KAVDHHDAEDLLLTKDNHRLLLQCKHWKTRKVGEMPVRELYGSMAAQSATGGVMLCCG
                     VFTLEATRFANFGGIELLDGPRLRSLLQRRPADGAHSPATRSMAG"
     gene            1032..2219
                     /locus_tag="VNZ06_13780"
     CDS             1032..2219
                     /locus_tag="VNZ06_13780"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWW21864.1"
                     /translation="MALRDWIQTARKRGASDLHLEAGCAVVARVRGELQPIGEPVAGA
                     DLKRMGQELLGEEGWQQFLGRGSADLSTMLGGSRCRINLYQTVRGLAMAIRLLSSSVN
                     DLRGCNLHPDLRTLLQAHSGLVVVSGPTGCGKSTTLAALIEEINLSRACHIITLDSPL
                     EYLFTNRRSYIRQREIPTHSPTFEQAIIDSLRENPDVMVVSEMRSPEVMRLTLNAAET
                     GHLVLATMHSSNCAEAMSRICMSFAPEVQGSVRAQLADCLVGVLCQRLDYLEAQQLRL
                     PHCELMLASSAVRGTLRSGQLGQLSNVIQSGGEEGMWSFDRYQRWMSQQKDWVRASVV
                     AQVADDPLPAARAASLTPVAPAAKRAGAPSSSDSAAEIVIDENLDLNEIAELAKRIER
                     RTP"
     gene            complement(2221..2715)
                     /locus_tag="VNZ06_13785"
     CDS             complement(2221..2715)
                     /locus_tag="VNZ06_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21865.1"
                     /translation="MSTHVHIYDLKTGLFTGKSHHTNVREPAAIASFIDESTPPGHGA
                     YVGKYVDYLSKKVDVHTGLLVEYQPPQPSRKHTWNPEAKRWQIKSREQRELTLKRIAE
                     LESSTHRAVRETLIRACEALDAIKEAVASKSPEVREAVDKLYSCVARLEVSEAELEEL
                     RRDL"
     gene            3059..3973
                     /locus_tag="VNZ06_13790"
     CDS             3059..3973
                     /locus_tag="VNZ06_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWW21866.1"
                     /translation="MQALSLLSLSILFAASCLGLGAGAADAGQSVMPAPFAESWHDGL
                     HDEPIMQVQRVDADTYVLRQSVKTNFEAPFMFLFFGHERALLMDTGAGGLKIRPTIDQ
                     LISQWMAEKGLASLQLVVAHTHSHGDHIAGDGEFSDRPNTVVVGHSPQQVAAFFHIRS
                     WPNDIAVFDLGGRELNIIPMPGHEKAEIGLFDRHTHLLLTGDALYPGRLYIPTDPSNQ
                     FPAYRDSIDGVVNYTRSLQVWWILGNHIEMTDKPGRDYAMHTPTHPQEHVLQLPYSSL
                     LELQKAVDAMGENARLEVHDDFIIYPLP"
     gene            complement(3958..4758)
                     /locus_tag="VNZ06_13795"
     CDS             complement(3958..4758)
                     /locus_tag="VNZ06_13795"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWW21867.1"
                     /translation="MTRALRGTFLALLALFLSAPLIVVTGVSFNGNARMNFPPQHVSV
                     IWYGNFFSDAGWRSAFTLSLVIAALSSLVALSIALPLAYAVWRHGSRAARALQALARI
                     SFLLPSVVVSIVFLVFWSAIGHAGHIEDTILSHAVVFVTLPLTTIGLGFQSIDPALID
                     AARTLGARDSEVLRTVIRPILLPYAICGLVFVFILSLNEYIIAYMVAGFEVETLPIKV
                     FNNLRMGFTPTMCVGAVLFMLLGVLGFGVIALIGDLPKLLGGRSQGNG"
     gene            complement(4755..5591)
                     /locus_tag="VNZ06_13800"
     CDS             complement(4755..5591)
                     /locus_tag="VNZ06_13800"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWW21868.1"
                     /translation="MKRARLSLLLLWPGLVTLSLTVVPVLLLLRISLAPQDPSAPWGT
                     GVSSEAYASLLAADSLRSLGRSLELALSVALISLGAGFPLAYCITRMRRRVQVLWLVF
                     LLATLTLSDVLIAFSWQVLLSKRIGLSKLLVALGLMDQPDSLAPSVGGVIGCLVYLVI
                     PFTVLTLYPSLSQLDRGLIEAARTLGAPAWRSFTSVVIPLTRVPAVIAFVLSAVLTLG
                     SFVPPLALGRPEHWPMSVLIGNAALAGHNLPRASAMSVLLLLVTALLAWGAAQTLRRR
                     AA"
     gene            complement(5588..6658)
                     /locus_tag="VNZ06_13805"
     CDS             complement(5588..6658)
                     /locus_tag="VNZ06_13805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWW21869.1"
                     /translation="MSIGLEVRQLSKHYGAQRVVSDVSLSVPNGEFVCFLGPSGCGKT
                     TLLRMIAGLEVPSAGRILMNHQDITDTPVHQRNFAMVFQALALFPFLNVEENIAYSLR
                     FRTINAQQRHEMVRDLLALIRLPDIGQRRIDQLSGGQRQRVAIARALAQEPMLFLLDE
                     PLSALDAKLRDHMQVELRQLQQKLHITTVLVTHDQREAMSLADTLVVMADGRIQQIGS
                     PTQIYRQPANRFVASFIGRSNLIDAQVEDRWRVNVGRASIGPLSVPRDLPAGAGVTLA
                     VRPEDVRVLTAGDTTPNCLPGRVTFVRDVGNEIELRIDCDGREIIAATSPAAWAALSS
                     TIHGVGSIGVLLPPESCHLLSS"
     gene            complement(6672..>6902)
                     /locus_tag="VNZ06_13810"
     CDS             complement(6672..>6902)
                     /locus_tag="VNZ06_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21870.1"
                     /translation="IDEAQEFINFCCTPEAQQLIARFVGSAPVLDRKLTNLTDQEFLT
                     VSSNTRPIYAATEARFQFTDYMEQQFTRMLTA"
BASE COUNT         1355 a         2166 c         2160 g         1221 t
ORIGIN      
        1 tcgatcagat tggcccacac cgtgaaccag ttttccggaa acgctgccgc ctgctccagg
       61 ctcaacccgt tgggcaccgg cagcgcgtga ctggcgcagg cgacgcagaa ctgcgcatag
      121 ccgccgccat gtaccagcgc gcctacctga tcgcccactc tccactgcga cacgttcggt
      181 cccagcgcgg ccacgcggcc tgagacttcg aggccgagta tcggtgaggc atcttgcggg
      241 gggttgtatc gccccatgcg ttgcagaaca tccggtcgat tgaccccagc gaatttgacc
      301 tcgatcagaa tctgaccgtc gccaggcacc ggagtcgggc cgtcaacaat ccgcaggctg
      361 tcagctgacg cttcaggacc aaaattgacc agtttcatca ctccaaggct gccagtgccg
      421 cgcgcatttg acaatgtgtc tcgcgcacga cagccgcgag cgcagatctg cgaccagaat
      481 cgtatccctt ggcaggccgc tcagccgacg atgagcgact acgacagaca tcgtgaaagg
      541 atctatggcc tcgaagaaag aactctctgc cgctgccgcc gacacgttgc tgaaggtcga
      601 tggactgtca caatcggatg tcgaacgcct ggtcacggcc tcgtatcgcg tgcgcggcta
      661 cgaggtgcgc gcggtgaaag cggtcgatca tcacgacgct gaggatctgc tgctgaccaa
      721 ggacaatcat cgcctgttac ttcagtgcaa gcactggaag acgcgcaagg tcggagagat
      781 gccggtgcgc gaactgtacg gttcgatggc ggcgcaatcg gccaccggcg gcgtgatgct
      841 ctgctgcggc gtgttcacgc tggaagcgac ccgatttgcc aatttcggcg gcatcgagct
      901 gctggatggc cccaggctcc ggtccctgct gcagcgtcgg cctgccgatg gcgcccattc
      961 acctgcgaca cgctctatgg caggctgagc gtcagcggaa ctttccgcca ggaagcagga
     1021 ctgaaggctt cgtggcattg cgagactgga ttcagacggc gcgcaagcgc ggcgcgagtg
     1081 acttgcacct ggaagcgggc tgcgccgtgg tcgcccgtgt gcgcggcgaa ctgcagccga
     1141 tcggcgagcc ggttgccggc gctgatctga agaggatggg acaggagctg ctcggcgaag
     1201 agggctggca gcagtttctg ggccgcggtt ccgccgacct gtcaacgatg ctgggcggat
     1261 cgcgctgtcg catcaatctg tatcagactg tgcgcggtct ggcgatggcg atccggctgc
     1321 tgagcagctc ggtcaatgat ctgcgcggct gcaatctgca ccctgacctg cgcacgctgc
     1381 tgcaggcgca ctccggtctg gtggtggtgt caggtcccac cggatgcggc aaatccacga
     1441 cgctggcggc gctgatcgag gagatcaatc tctcacgtgc ctgccacatc atcacactgg
     1501 atagcccgct ggagtacctg tttaccaacc gccgctcgta catccgtcag cgcgagatcc
     1561 caacgcattc gcccacgttc gagcaggcca tcatcgactc gctgcgcgag aaccccgacg
     1621 tgatggtggt gagcgagatg cgctcgcccg aggtcatgcg actgacgctc aatgccgcgg
     1681 agaccggaca tctggtgctg gcgaccatgc attcctccaa ctgcgccgaa gcgatgagcc
     1741 gcatctgcat gtctttcgcg ccggaggtcc agggcagcgt gcgcgcacaa ctggccgact
     1801 gcctggtcgg tgtgctgtgt cagcggctgg actatctgga agcccagcag ttgcggctac
     1861 cgcactgtga gctgatgctg gccagctccg cggtgcgggg cacccttcgc tcaggccagt
     1921 taggtcaact ctcgaacgtc atccaatccg gcggcgaaga gggtatgtgg agcttcgatc
     1981 ggtatcagcg ctggatgagc cagcagaaag actgggtgcg tgcttcggtc gtcgcccagg
     2041 tagcggatga tccgcttccg gcagcgcgcg ccgcgtcgct cacgccggtg gcgccggctg
     2101 ccaaacgcgc tggcgccccg agtagttccg acagcgccgc ggagatcgtg atcgatgaga
     2161 acctggatct gaacgagatc gcggagctcg cgaaacgtat cgaacgacgc acgccctagg
     2221 ctagaggtcc cggcgcagct cctcgagctc agcctcgctg acttccagtc gcgccacaca
     2281 ggagtagagc ttgtccacgg cctcgcgcac ttcgggcgac ttggaggcaa ccgcctcctt
     2341 gatggcatcg agggcttcac aggcgcgaat caacgtctcg cgcaccgcgc gatgcgtgct
     2401 cgattccagt tcagcgatgc gcttgagggt gagctcgcgc tgctcgcggc tcttgatctg
     2461 ccagcgcttg gcttctggat tccaggtgtg tttgcgcgat ggctggggcg gctggtattc
     2521 caccagcaat ccggtgtgaa catccacctt cttggatagg tagtccacgt atttgccgac
     2581 gtaggcgccg tgccctggcg gagtggactc atcgatgaag gaggcgattg ccgccggctc
     2641 cctcacattg gtgtgatgcg atttaccggt gaacagcccg gtcttcaggt cataaatatg
     2701 aacatgtgta ctcacgattc gtgtcccgcg gcgtaaattt atttgatttt cggggccgcc
     2761 ctccgcagcc cggaaactag catgcttaac aataatccca caaccggaga accaagaatg
     2821 ccggctatcc atttgatctt caattgattt ggcccaatct gcgacggacg tcaatcaata
     2881 cttgtgcaca ccggcaagta gaagccattt gtaatcaatc ggcggtatcc atcggtgctg
     2941 tcacaggtta cgccgatccg ccgtatgttc tcacgcaagt tcggttgcac gccccgacat
     3001 tcgtccgatc ccatgcgggt gctaagtttc accatcgcaa tcgccgcagt cggtgcccat
     3061 gcaagctttg agtttgctca gcctatcgat cctgttcgcc gcgagctgcc tggggctcgg
     3121 cgccggagcc gccgatgccg gccagagcgt catgcctgcg ccgttcgctg aaagctggca
     3181 tgacggcctt cacgacgagc cgatcatgca ggtgcagcgg gtcgatgcgg atacctatgt
     3241 actgcgccag tcggtcaaga ccaatttcga ggcccctttc atgttccttt tcttcggaca
     3301 tgaacgcgca ttgctgatgg acacgggagc cggaggcctg aagataaggc cgaccatcga
     3361 tcagctgatc agccagtgga tggcggaaaa aggacttgca tcgctgcaac tggtcgtcgc
     3421 ccacacccat tcacatggtg atcacatcgc cggtgacggc gaattcagcg accggccgaa
     3481 cacagtcgtt gtcggccaca gtccccagca ggtcgcggcc ttctttcata tccgctcgtg
     3541 gccgaacgat atcgcggtgt ttgatctcgg cggacgcgag ctgaacatca tcccgatgcc
     3601 tgggcacgaa aaggcagaga tcggtctgtt cgaccgtcac acgcacctgt tgctgacggg
     3661 cgatgcgctc tacccggggc ggctgtacat ccccaccgat ccgagcaatc agtttccggc
     3721 ctatcgcgac agcatcgatg gcgtggtcaa ctacacgcgc tcactgcagg tttggtggat
     3781 cctggggaat cacatcgaga tgacagacaa gccagggcgt gactatgcga tgcacacgcc
     3841 gacgcacccg caggagcacg tgctgcagct gccctattcg agcctgctgg agttgcagaa
     3901 ggcggtggat gcgatgggcg agaatgcgcg gcttgaggtg cacgacgact tcatcatcta
     3961 tccattgccc tgagagcggc ctccgagcag cttcggcaga tcgccgatca gggcgatcac
     4021 cccgaagccc agcacgccca gcagcatgaa caggacggcc ccgacacaca tcgtcggggt
     4081 gaaacccatg cgcaggttgt tgaacacttt gatcggcagt gtctcgacct cgaagccggc
     4141 caccatgtag gcgatgatgt attcgttgag actcaggatg aagacgaata ccaggccgca
     4201 gatcgcgtaa ggcagcagga tcgggcggat cacggtgcgc agcacttcgc tgtcgcgcgc
     4261 cccgagcgta cgcgccgcat cgatcagcgc cgggtcgatc gactggaatc cgaggccgat
     4321 cgtggtcagc ggcagcgtca cgaataccac ggcgtgactc aggatcgtgt cttcgatgtg
     4381 gccggcgtgg ccgattgcgc tccagaagac cagaaaaacg atcgacacca ccaccgacgg
     4441 cagcagaaaa gaaatgcgcg ccaaagcctg cagtgcccgc gccgcgcgcg atccatggcg
     4501 ccacaccgca taggccagcg gcagtgcaat gctgagcgct accagcgatg acagcgcggc
     4561 aatgaccaac gaaagcgtga acgctgacct ccagccggca tcgctgaaga aattcccgta
     4621 ccagatgacg ctgacgtgct gcggcgggaa attcatgcgc gcgttgccgt tgaagctgac
     4681 gccggtcacc acgatcagcg gtgccgacag aaacagcgcc agcagcgcca gaaatgtacc
     4741 tcgcagcgcc ctcgtcatgc cgcccgccgg cgcagcgtct gcgccgcgcc ccacgccagc
     4801 agtgccgtca ccagcagcag cagcaccgac atcgccgagg ctcgtggcag attgtgtccc
     4861 gccagtgcgg cattgccgat cagtaccgac atcggccagt gttcgggccg gccgagggcc
     4921 agcggcggga cgaacgagcc gagggtcagt accgctgaca ggacgaacgc gatcacggcc
     4981 ggcacacgtg tgagcggaat gaccaccgag gtgaagctgc gccacgcggg cgcccccagg
     5041 gtacgtgctg cctcgatcag tccgcggtcc agctgcgaca acgacgggta cagcgtgagc
     5101 acggtgaacg gaatcaccag ataaaccaga cagccgatga cgccaccgac actcggagcc
     5161 agcgaatcgg gctggtccat gagtcccagc gcgaccaaca gcttggacag tccgatgcgc
     5221 ttcgacagca gcacctgcca cgagaacgcg atcaatacat ccgacagggt cagcgtcgcg
     5281 agcagaaata ccagccacaa cacttgtacc cgccggcgca tccgggtgat gcaataggcg
     5341 agcggaaagc cagcgccgag actgatcagc gccacgctca gcgccagttc cagcgagcgc
     5401 cccaggctcc tgagcgaatc ggccgccaac aggcttgcgt aggcttcgga cgagacaccc
     5461 gtgccccacg gcgccgaggg gtcctgtggc gcgaggctga tgcgtaacag cagcagcact
     5521 ggcaccacgg tcagcgacag cgtcaccagc cccggccaca gcagcagcag gctcaggcga
     5581 gcacgcttca tgagctgagc aagtgacagc tctcgggcgg cagcagcacg ccgatcgagc
     5641 ccacaccgtg gatcgtcgag ctcagcgcag cccaggccgc aggtgaggtg gccgcgatga
     5701 tctcccgtcc gtcgcagtcg atgcgcaact cgatttcatt ccccacatcg cgcacgaagg
     5761 tgacccggcc gggcaggcag ttcggggtcg tatcgccggc cgtgagcacc cgtacgtcct
     5821 caggtcgcac cgcgagcgtc acgccggcac cggcaggcag gtcgcgtgga accgaaagcg
     5881 ggccgatcga tgcgcggccg acattcacgc gccaccggtc ctcgacctgc gcgtcgatca
     5941 ggttgctgcg accgatgaag ctggcgacaa agcgattggc gggttgacgg tagatctgcg
     6001 tcgggctgcc gatctgctga atccggccgt cagccatcac gacaagggtg tcggcgaggc
     6061 tcatcgcctc gcgctgatcg tgggtcacca gaaccgtcgt gatatgcagc ttttgttgca
     6121 gctggcgcag ttccacctgc atgtgatcac gcagcttggc gtccagtgcc gacaacggtt
     6181 catcgagcag aaacagcatc ggctcctgcg ccagggcccg ggcgatcgcc acccgctggc
     6241 gctgtccgcc cgacagctga tcgatccggc gctggccaat gtccggcagt cggatcagcg
     6301 ccagcagatc gcgcaccatt tcgtgacgtt gttgcgcgtt tatggtgcga aagcgcaggc
     6361 tgtacgcgat gttctcctcg acgttgagga acgggaacag cgccagtgcc tgaaacacca
     6421 tggcgaagtt acgctggtgc acgggcgtgt cggtgatgtc ctgatggttc atcagaatgc
     6481 ggcccgcgct cgggacctcc aggccggcga tcatccgtag cagcgtggtc ttgccgcagc
     6541 ccgaaggtcc cagaaagcag acaaactcac cattcggaac gctcagtgac acatcgctga
     6601 cgacgcgctg cgcgccgtag tgcttgctga gctggcgcac ctccagcccg atggacatgc
     6661 ggcgtggccc ttcaggcagt cagcattcgg gtgaactgct gttccatgta gtcggtgaat
     6721 tgaaagcgcg cttcggtcgc ggcgtagatc ggtcgggtgt tcgaggagac ggtgaggaat
     6781 tcctgatcag tgaggttggt gagcttccga tccagcactg gcgcggaccc gacgaaacgg
     6841 gcgatgagct gctgtgcctc gggcgtgcag cagaaattga tgaattcctg tgcctcatcg
     6901 at
//
LOCUS       DATMRM010000455         7356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3655076, whole genome shotgun sequence.
ACCESSION   DATMRM010000455 DATMRM010000000
VERSION     DATMRM010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3655076"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VNZ06_13815"
     CDS             <1..244
                     /locus_tag="VNZ06_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21871.1"
                     /translation="PPDSGFALIPEPSREPPPTRAQLRGRTLGAALTFLLGLFAAAYL
                     GRPGGNRIAATLAALLLIVGLCWLIVTILQNVTARK"
     gene            complement(258..1343)
                     /locus_tag="VNZ06_13820"
     CDS             complement(258..1343)
                     /locus_tag="VNZ06_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HWW21872.1"
                     /translation="MGRPPGGAIAYLHLADLGAPPEFAHSGVAGIMDLTAALQWVREN
                     IENFGGDPGNVMIFGQSGGGAKTSVLLATPKAKGLFHRAAVQSGSLLRLTPRERASAH
                     AEQLLQQLQMPVSRISELQQLPWQQILEAQAVVFGVNPFGLAPVLDGDYLPHEPFEPQ
                     APPESADIPMIVSSTLEDAGLVLTQFGLTESAPQTTLQERFQDQAAPMLSLYRKHYPD
                     KSPYRMLAMILTDGGFRRFAIQQAERKAAQASAPVYMYQWDWESAAFGGRYGATHGLD
                     VGASFREARAGNDEARVAHELSSAWVAFARSGNPNTPALPSWPSYDIRTRATMVFDEP
                     TTHLQNDPRSQLRQFWEKMPQFASLIG"
     gene            complement(1446..2561)
                     /locus_tag="VNZ06_13825"
     CDS             complement(1446..2561)
                     /locus_tag="VNZ06_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HWW21873.1"
                     /translation="MSRRAKAVVSRQVGAPVVVETIEVQEPRHNEVTVQMMACGVCHS
                     DLSATNGTINYPLPMVLGHEGAGVIVAIGEGVTRYKVGDHVISSFVNMCGHCHYCQTG
                     RPQLCVESLKARFTLPDGTVRTYDSSGSALNVFSGCGVMAEFCTLHEDSVVKVDKEMP
                     LDKAALISCGVMTGVGAATNTARVQAGSVCMVFGCGGVGLNAIQGCVISGASMVVAVD
                     TSDEKLDLARKFGATHVFNVTGQDSPGKALFKMTGGGADYAFDCVGVGKVSEQAWNVL
                     RRGGTAVIVGISNAKDQIALNAQMVATSEKVLTGSYYGSARPELDFPRLIALYRKGHL
                     KLDELITQTYTIDEAPQAFAALDRGRQGRGVILFAHA"
     gene            complement(2592..3734)
                     /locus_tag="VNZ06_13830"
     CDS             complement(2592..3734)
                     /locus_tag="VNZ06_13830"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWW21874.1"
                     /translation="MDFEFTDEQRQLREALERFLIKEYSFDRYRAIKASPQGWDKQVW
                     HQLAELGVLGVNVPSEQGGLGFGPLETLAIMDVCGPSMLLEPLTSSAVIATTLLNGFV
                     ADSAVSELLRHLAAGEQIAVLSHHDADARSELQWVSASARRAGNGYVLNGHKAVALHA
                     NLADTLLISARTAGQPGDARGISLFRVPHDAAGVTLIGYPTMGGQLAAEVVLKDVQVP
                     GTHRLGEEGKVLPAIESALDVGLAALCAESVGVMQALFKATVEYLRTRQQFGQPIGRF
                     QALQHRVADMLIHLEQARSMSYLAAMRCISTDLPERRRALSAAKIIVGQAGRFIGQQA
                     VQLHGGMGMTEELNVSHLFKRLTASEFLFGDTDSHLQKLAALTRST"
     gene            complement(3749..4954)
                     /locus_tag="VNZ06_13835"
     CDS             complement(3749..4954)
                     /locus_tag="VNZ06_13835"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWW21875.1"
                     /translation="MHFELNDEELAFRDSVREFVRTKLPESISRKIFEGQDVSRDDMV
                     TWQKVLAAQGWGGVGWPKQFGGPGWSAMQMHIFDEESALAGAPRLNPFGLRMVAPVIM
                     AFGTAQQQQRYLPKIASGEEWWCQGYSEPGSGSDLASLKTRAVRDGDSYVVSGQKTWN
                     TLGQFADWIFCLVRTDPQAKLQEGISFLLISMKTPGVTLRPIKLLDGSVEVNDVFFDQ
                     VRVPIENRIGDENRGWTYAKFLLGHERTNIAGVGIAKRELRRLRRIAGEERKNGKPLI
                     DDPLFAARVAQIEADLMALELTTLRTISAEKNKRAPGPEASVLKVRGTEIQQAISELT
                     MLAVGPYALPWRREALEAGYQVDPNAEVGPPYAATAASRYFNWRKLSIFGGTNEIQKN
                     IISKMMVGL"
     gene            5088..5945
                     /locus_tag="VNZ06_13840"
     CDS             5088..5945
                     /locus_tag="VNZ06_13840"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWW21876.1"
                     /translation="MERQARIRKIAAHITPHPAFPRKSAGTLPDRHFVTALARGLEVL
                     ACFRHGERWLGNQELARRCALAKSTVSRLTHTLTRLGYLIHDKEQSKYGLGPAIITLG
                     SSMLSRLDIRKLAHPKMQELAEFAQCWVSLGSRDRLSMISIDAVHGSAAVRFRLDSGS
                     RIHIASSAMGRAYLAAVTQDERDELMRRVRERADVDVNRWPELRRGVVKALRDIRELG
                     VCTSFGEWKQGINAIAIPVRPGGGLPPMAISCGAPAYMVSKEFMLDSVRPRLIALANE
                     LEDSLGTHT"
     gene            6056..6823
                     /locus_tag="VNZ06_13845"
     CDS             6056..6823
                     /locus_tag="VNZ06_13845"
                     /inference="COORDINATES: protein motif:HMM:NF006070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWW21877.1"
                     /translation="MTVREMFELNGRVALVTGGSRGLGLQIATALGEMGAKIAISARK
                     AEELAEARQTLEAKGIETFTEVNDLSDPRQAQPLCDAVASHYGTIDILVNNAGTTWGA
                     RAEEHPLPAWSKVMTLNTTSVFALSQAVANRCFIPRRSGNIIVISSTAALRVGGLKAV
                     AYSASKAGALHLARALAIEWGQYNIRVNAICPGFFPSKMTSGLLKDIEKQVIERTPLG
                     RMGDDTDLMGAAVFLASNASRHVTGQYIAVDGGSTSI"
     gene            6820..>7356
                     /locus_tag="VNZ06_13850"
     CDS             6820..>7356
                     /locus_tag="VNZ06_13850"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWW21878.1"
                     /translation="MTERVVPLEETSGGLRRPPPQLAFDPAPLGAWMAANVPGFAGPI
                     NVLQFAGGQSNPTFLIETPTRRYVMRRKPPGKLLPSAHAVEREYRVIAALGATRVPVA
                     RAYGLCEDISVIGSAFYIMEYVQGRIFWDAALPEVPPAGRRAVYLEMIRVLAELHSVD
                     YQAVGLGDYGKPDHYIERR"
BASE COUNT         1328 a         2389 c         2313 g         1326 t
ORIGIN      
        1 cccgcccgac tcgggattcg cgctgattcc agaaccctcg cgtgagcctc cgccgacacg
       61 tgcgcagctg cgagggcgga cgctgggggc cgcgctgaca tttctgctag gactgttcgc
      121 agccgcctac ctgggccgtc ctggcggcaa ccggattgcc gcgaccctgg cggcgctgct
      181 gctgatcgtg ggactgtgct ggctgatcgt gacgatcctg cagaacgtga cggcgcgcaa
      241 ataggcgccg aaagcaccta accgatcagg ctcgcgaact gcggcatctt ctcccagaac
      301 tgacgcagct gcgagcgcgg gtcgttctgc agatgcgtcg tcggctcatc aaacaccatc
      361 gtcgcgcgcg tgcgaatgtc gtagctcggc caggacggca gcgccggtgt gttcggattg
      421 ccgctgcgcg cgaacgccac ccaggccgat gagagctcgt gtgcgacgcg cgcctcgtca
      481 ttgccagcgc gcgcctcgcg gaacgatgca ccgacgtcca gtccgtgcgt ggcgccatag
      541 cgcccaccga atgccgccga ctcccaatcc cattgataca tataaaccgg agcgctcgcc
      601 tgcgcggcct tgcgctcggc ctgttggatc gcaaagcgcc ggaagcctcc atcggtcagg
      661 atcatcgcca gcatccgata gggcgactta tccggatagt gcttgcggta cagcgacagc
      721 atcggtgcgg cctgatcctg gaagcgctcc tgcagcgtgg tctgcggtgc cgactcagtg
      781 agcccgaact gggtgagcac cagacctgca tcctcgagtg tcgatgaaac gatcatcggg
      841 atgtcggccg attcgggcgg cgcctgcggc tcgaacggct cgtgtggaag gtagtcgcca
      901 tcaagcaccg gcgccaggcc gaacgggttg acaccaaaga ccacggcttg cgcttccaga
      961 atctgttgcc acggaagctg ctgcagctcg ctgatgcgac tgaccggcat ctgcagctgc
     1021 tgcagcagct gctcggcgtg cgcgctggcg cgctcgcgcg gcgtcagacg cagcaatgat
     1081 ccgctctgta ccgccgctcg atggaacagg cccttggcct tcggcgttgc cagcagcacg
     1141 ctggtcttcg cgccgccgcc ggactgaccg aagatcatca cgttgcccgg atcgccaccg
     1201 aaattctcga tgttctcgcg cacccattgc agcgcggcgg tcagatccat gatgccggcg
     1261 acaccggagt gagcaaattc cggcggtgcg cccagatcgg ccaggtgcag ataagcgatt
     1321 gcgccccccg gtgggcgccc catagggaag gcccttgaac tgcttgatgc cggtgctttg
     1381 gatgccctgc acgcggccgg aagcggtctg cgtctcaacg aacagtttgg gacgcgaggg
     1441 ctcgctcatg cgtgagcgaa caggatcacg ccgcgaccct gcctgccgcg atccagcgcc
     1501 gcgaaggcct gcggcgcctc atcgatggta taggtctgcg tgatcagctc atcgagcttc
     1561 agatgtccct tgcgatacag tgcaatcagc cgcgggaaat ccagctccgg acgggctgag
     1621 ccgtagtaac tgccggtcag cactttttcg ctggtggcga ccatctgcgc attgagcgcg
     1681 atctgatcct tggcattgga aatgccgacg atgaccgcgg tgccgccacg ccgcagcaca
     1741 ttccacgcct gctcagagac cttgccgacg ccgacgcaat cgaacgcgta gtccgcacca
     1801 ccgccggtca tcttgaagag cgccttgccg gggctgtcct gaccggtcac gttgaagacg
     1861 tgcgtcgcgc cgaacttgcg cgccagatcc agcttctcat cgctggtgtc cacggctacg
     1921 accatgctgg caccggagat cacgcagccc tgaatcgcgt tcagtccgac gcccccgcag
     1981 ccgaacacca tgcacaccga gccggcctgc acacgcgcgg tgttggtggc cgcgcccacg
     2041 ccggtcatca cgccgcagga aatcagcgcg gctttatcca gcggcatctc cttgtccacc
     2101 ttgaccacgc tgtcctcgtg cagcgtgcag aactcggcca tcacgccgca accgctgaac
     2161 acgttgagcg ccgagccgct cgaatcgtag gtgcgaaccg tgccatcggg cagcgtgaag
     2221 cgcgctttga gagactcgac gcacagctgc ggacggccgg tctgacagta gtggcagtgg
     2281 ccgcacatgt tgacgaagga gctgatgaca tgatcgccga ccttgtagcg cgtgacgccc
     2341 tcgccgatcg cgacgatcac ccccgctcct tcatggccca gcaccatcgg caagggatag
     2401 ttgatggtac cgttggtggc ggacaaatca ctgtggcaga cgccacaggc catcatctgg
     2461 accgtcacct cattgtgacg cggctcctga acctcgatgg tttcaacgac tacgggcgca
     2521 ccgacctggc gcgacacgac cgccttggct cgtctgctca tggctgctcg atccccttca
     2581 atttaagacg ttcaggtact gcgcgtgagg gccgctagtt tctgcaggtg gctatccgta
     2641 tccccgaaca ggaactcgct ggccgtgagt cgtttgaaca ggtggctcac gttgagttcc
     2701 tcggtcatcc ccatgccgcc gtgcaattgt acggcctgct ggccgatgaa gcgcccggcc
     2761 tggccgacaa tgattttggc ggccgacagc gcccgccgtc gctctggcag atccgtggag
     2821 atgcagcgca tcgcggcgag atagctcatc gagcgggcct gctccagatg aatcagcata
     2881 tcggccacgc ggtgctgcag tgcctggaac cggccgatcg gctgaccaaa ctgctggcgc
     2941 gtgcgcaggt actccacggt cgccttgaac agcgcctgca tcacaccgac cgattcggcg
     3001 cacagggctg ccagacccac atcgagcgcg gattcgatcg ccggaaggac cttaccctct
     3061 tcacccagcc ggtgggtgcc gggcacctgc acgtccttga gcacgacctc ggccgcgagc
     3121 tgcccgccca tggtcgggta gccgatcagt gtcacaccgg ccgcatcgtg cggcacgcga
     3181 aacagcgaga tgccccgggc atccccgggc tgaccggcag tgcgcgccga gatcagcagc
     3241 gtatcggcga gattggcatg cagcgccacg gccttgtggc cgttgagcac gtagccgttg
     3301 ccggcgcgcc tggcgctggc gctgacccac tgcagctccg agcgcgcgtc ggcgtcatgg
     3361 tgcgacagca cggcgatctg ttcgccggcc gccaggtgcc gcagcagctc tgaaacggca
     3421 ctgtcggcga cgaagccgtt cagcagcgtg gtggcaatca cggcgctgct ggtcagcggc
     3481 tcgagcagca tgctcggacc gcacacgtcc atgatcgcca gggtctcgag cggaccgaat
     3541 cccaaaccgc cctgctcgct gggcacgttc acacccagca cgccgagttc ggccagctgg
     3601 tgccagacct gcttgtccca accttgcggt gaagccttga tggcccgata gcgatcgaag
     3661 ctgtattcct tgatcagaaa gcgctcgagc gcctcgcgta gctggcgctg ctcgtcagtg
     3721 aactcaaaat ccatcgcgac tcaagctcct acagaccgac catcatcttg gagatgatgt
     3781 tcttctgaat ttcattggtg ccgccgaata tcgacagctt gcgccagttg aagtagcgcg
     3841 atgcggcggt agccgcgtag ggcggcccga cctcggcgtt cgggtccacc tggtagcccg
     3901 cctccagcgc ctcgcgccgc cacggcagcg cgtagggccc gactgcgagc atcgtcagct
     3961 cggagatcgc ctgctggatc tcagtgccgc gcactttcag caccgaggct tccggaccgg
     4021 gcgcacgctt gttcttctcg gcggaaatcg tgcgcaatgt ggtcagctcg agggccatca
     4081 ggtccgcttc gatctgcgcc acgcgcgcgg cgaacagcgg gtcatcgatc agcggcttgc
     4141 cgttcttgcg ctcctcgccg gcaatgcgcc gcagccgtct gagctcgcgc ttggcgatgc
     4201 cgaccccggc gatgttggtg cgctcgtgac cgagcaggaa tttggcgtac gtccagccgc
     4261 ggttctcatc accgatgcga ttttcgatcg gcacgcgtac ctggtcgaag aagacatcgt
     4321 tcacctcgac gctgccgtcg agcagtttga tcgggcgcag cgtcacgccg ggagtcttca
     4381 tactgatgag caggaaggag atgccctcct gcagcttcgc ctggggatcg gtgcgcacca
     4441 ggcagaagat ccagtcggcg aactgcccga gcgtgttcca ggtcttctgg ccgctgacga
     4501 catagctgtc gccatcgcgc accgcgcgcg tcttgagcga agcaagatcc gaacccgagc
     4561 caggctcgga atagccctga caccaccact cctcgcccga ggcgatcttg ggcagataac
     4621 gctgctgctg ctgcgcagtg ccgaacgcca tgatgacggg cgccaccatg cgcaagccaa
     4681 acggattcag acgcggggcg cccgcgagcg cgctctcctc gtcgaagata tgcatctgca
     4741 tcgcgctcca gcccggacca ccaaactgct tcggccaacc gactccgccc cagccctgag
     4801 cggcgagcac cttctgccag gtgaccatgt catcgcgact gacgtcctgg ccttcgaaga
     4861 tcttgcgact gatcgactcc ggcagtttgg tgcgcacaaa ttcgcgcacc gaatcgcgaa
     4921 aggccagctc ttcgtcgttc agctcgaaat gcatggatgt gacctcagga gactctccgc
     4981 agcggcggcg atgaaattgc cgcgcttgag aaattcagta ctgcagagca gaatttagtt
     5041 ccaaattcag actcgcaaat tatcctcagc cgatccttac cttgtcaatg gaacgccagg
     5101 ctcgcatccg caaaattgcg gcgcacatca ctccgcatcc ggctttccca cgcaagtccg
     5161 cggggaccct gcccgatcgg catttcgtga ccgcactggc ccgggggctc gaagtgctcg
     5221 cctgctttcg tcacggcgag cgctggctcg gcaaccagga gctcgcgcgg cgctgcgcac
     5281 tcgcgaaatc caccgtttcg cgcctgacgc acacgctgac gcggctgggc tatttgattc
     5341 atgacaagga gcagtcgaag tacgggctcg ggccggccat catcacgctc ggcagctcga
     5401 tgctgtcgcg gctggatatt cgcaagctcg cgcacccgaa aatgcaggag ttggccgaat
     5461 tcgcgcaatg ctgggtaagc ctgggcagcc gcgatcggct ctcgatgatc tcaatcgatg
     5521 cggtgcacgg ctcggccgcg gtgaggttcc ggctcgatag cggctcgcgc attcacatcg
     5581 ccagttcggc gatgggccgc gcctacctgg cggcggtcac gcaggatgag cgcgacgagc
     5641 tgatgcggcg ggtgcgcgag cgggccgacg tcgacgtcaa tcgctggccc gaactgcgtc
     5701 gtggcgtggt caaagcgctg cgtgacatcc gcgagctggg ggtgtgcacc tcgttcggcg
     5761 agtggaaaca ggggatcaac gcgattgcca ttccggtccg gccgggaggc gggctgccgc
     5821 cgatggcgat cagctgcggg gcgccggctt atatggtgtc caaggagttt atgttggatt
     5881 cggtgcgtcc gagactgatc gcgctggcca atgaactgga ggattcgctc ggtacacata
     5941 cttagacatg ttctatttat taaacttcct gcatactaaa aactcgggtg gcttaactca
     6001 cgctcgcatg agctccaagc gaaacgaccc ccgaaactac agaggttgag cgacaatgac
     6061 ggtgcgcgaa atgttcgagt tgaatggccg cgtggctctg gtcacgggtg gctcacgcgg
     6121 tctgggtctg cagatcgcga ccgctctggg cgagatgggc gcgaagattg ccatcagcgc
     6181 gcgcaaagcg gaggaactgg ccgaggccag gcagacgctc gaggccaagg gcatcgagac
     6241 tttcaccgag gtgaacgatc tgtcggaccc gcggcaggcg cagccgctgt gcgatgcggt
     6301 cgcatcgcac tacggcacga tcgacattct ggtcaacaac gccggcacca cctggggagc
     6361 acgcgcggaa gaacatccgc tgcccgcctg gagcaaagtg atgacgctga acaccaccag
     6421 tgtgttcgcg ctcagtcagg cggtcgccaa ccgctgcttc ataccgcgcc gctcgggcaa
     6481 catcatcgtg atttcctcga ccgcggcgct gcgggtcggc ggtctgaaag ctgtcgccta
     6541 cagcgcatcg aaagcgggtg cactgcacct ggcgcgggcg ctggcgatcg aatggggcca
     6601 gtacaatatt cgtgtgaacg ccatctgccc aggattcttc ccatcgaaga tgaccagcgg
     6661 actgctcaag gatatcgaga agcaggtgat cgagcgtacg ccgctcggtc gcatgggcga
     6721 tgacacggac ctcatgggcg ccgcagtgtt tctggcgtcg aacgcatcgc ggcacgtcac
     6781 cggtcaatat attgcggtcg acggaggatc gacatccata tgaccgaacg ggtcgtaccg
     6841 ctggaagaga catcgggcgg cttacgtcgc cctcccccgc aacttgcgtt cgatcccgcg
     6901 ccgctggggg cctggatggc cgcgaacgtt ccggggtttg ccggcccgat caatgtcttg
     6961 cagtttgcgg ggggtcaatc gaacccgacc tttttgatcg agacaccgac gcggcgctat
     7021 gtcatgcgcc gcaagccccc gggcaaactg ctgccctcgg cgcatgcggt ggagcgcgag
     7081 tatcgggtaa tcgcggcgct gggcgccacg cgcgtgccgg tggccagagc ctatggcctg
     7141 tgcgaagaca tctcggtgat cggcagcgcc ttctacatca tggaatatgt gcagggccgc
     7201 atcttctggg atgccgcact gcctgaagtg ccgcctgccg ggcgacgtgc ggtctatctg
     7261 gaaatgatcc gcgtgctggc cgagctgcat tcagtggatt accaggcggt gggattgggc
     7321 gactacggca agcccgatca ctacatcgaa cgccgg
//
LOCUS       DATMRM010000456         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1199955, whole genome shotgun sequence.
ACCESSION   DATMRM010000456 DATMRM010000000
VERSION     DATMRM010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199955"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(522..>2268)
                     /locus_tag="VNZ06_13855"
     CDS             complement(522..>2268)
                     /locus_tag="VNZ06_13855"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWW21879.1"
                     /translation="ADLTAESELHQRRLSLARAWQSLVRLPFVLGRLALELLPVLVFV
                     GVATMLLGSEIGDAPTARLVILAVVNAYALSRGLICVVRALAGPFGLFRVRAETAAYI
                     EIWARRILAVGVSGIAFANVALLLGLHHAGYTALLHLVMLIVHLFIVVIILQCRRQVA
                     EAIRAPAGREGVAARVRNRFAGLWHYLAIALDLALWAVWALNIRNGYSLLLQYFVGTI
                     AVAMITRLATIVVLGLIDRGFRISPDILQRFPGLETRANRYLPLLRKIVSTVTAFIGF
                     VALLEVWGVDAIVWFYGGQIGSRLLSAVVTIGVAALAAAAIWEASNALMDRQLNVLSR
                     DGHFARAARLRTFQPMLRTALLCLIVTVVGLTALSEIGVNVAPLLAGAGIVGIAIGFG
                     SQKLVQDLITGLFLLLENTVHVGDNVSVSGLSGTVENVSIRTIRLRAADGSVHIVPFS
                     AVTTITNSSRGAGNAAVSVNVSYKEDTDRAGQILKDIVAEMRREPEYRQAIRGDLELW
                     GVDKVDGSMASIVGQIRCTDSGRWPVQREFNRRMKRRFQECGIEIASAGQTILMQIPA
                     PADVASNSMPRRVAG"
BASE COUNT          485 a          704 c          702 g          377 t
ORIGIN      
        1 cgccagcaat ctgccaattc gggtgccacc tgcgaccacc cgtgctctgg ctgctgtccg
       61 acagcgggcg caatataggc gatttgaagc agaagttctg tgcttgaact gcaactcacg
      121 gtttttcaaa tatgactgca aaatggccgt tgatggatcg tttttgggct ccggaacttg
      181 caaagcaagc ttgctccagg gcggtcaatt tggcgattgg agatgccgtt tgctggggca
      241 tgagtccgtt tgtggcactt aacggtcatg gcgcgatgtc cgacctgagt ccgctctcgg
      301 tggcatgggt caagaccaac cgaacgaagt tagccgggtt caatcagtag gacggcgccg
      361 accaaactgc tgctggccgg gccgcagcac ctaagagggg cggcgacttc gccgcgtctc
      421 gaaggtttag ggtgtacagc tcatggttcg agacggcgca ccggacagca caacacgagc
      481 gctgccggtg cgcctcctca ccgtgaaggt ggaatttccg attagccagc cacccgtcga
      541 ggcatcgaat tcgaggctac gtcggccggc gcgggaatct gcatcagaat ggtttgacct
      601 gcggatgcga tttcgatgcc gcactcctgg aatcgtcgct tcatgcggcg gttgaattcg
      661 cgctgcactg gccagcgacc gctgtcggtg cagcgaattt ggccgacgat cgacgccatc
      721 gagccgtcca ccttgtcgac gccccacagt tcgagatcgc cgcggatcgc ctgtcggtat
      781 tcgggctcgc gacgcatttc agcgacgata tccttgagta tctggcccgc gcggtcggtg
      841 tcttccttgt aggaaacgtt gacgctgacg gccgcgttgc cggcaccacg gcttgaattg
      901 gtgatcgtcg tcacggcgct gaaaggaacg atgtgcaccg agccgtcggc ggcccgcaga
      961 cgaatggtgc ggatcgaaac attctcgact gtccctgaca ggccggacac gctgacgttg
     1021 tcaccgacat ggacggtatt ttccagcaag agaaacagcc cggtaatgag gtcctgcacc
     1081 agtttctgcg agccgaagcc gatggcgata ccgacgatcc cggcgccggc cagtagcggc
     1141 gcgacgttga cgccgatttc gctcagcgcc gtcaaaccaa ccaccgtgac gatcaggcac
     1201 agcagcgccg ttcgcagcat cggttgaaag gtacgcagtc gcgcggcgcg cgcgaagtga
     1261 ccgtcgcgcg acagcacatt gagctggcga tccatcagcg cgttgcttgc ctcccagatc
     1321 gccgccgccg caagcgcggc gacgccgatc gtcactaccg ccgacaacaa ccggctaccg
     1381 atctggccgc catagaacca gacgatggca tccacacccc agacttcaag cagtgcaacg
     1441 aagccgatga aggccgtgac agttgagacg atcttgcgca gtagcggcag atagcgattg
     1501 gcgcgggttt cgaggcccgg gaagcgctgc aggatatccg ggctgatcct gaaaccccga
     1561 tcgatcaggc ccagcaccac gatggtggcg agacgagtga tcattgcaac cgcaatggtg
     1621 ccaacaaaat actgcagcag cagcgagtaa ccgttgcgga tattcaacgc ccacacagcc
     1681 cacagtgcga ggtccaaggc gatcgcaaga tagtgccaca ggccggcgaa gcgattgcgc
     1741 accctcgccg cgacgccctc gcggccggcc ggcgcgcgaa tggcctcggc gacctgccgg
     1801 cggcactgca ggatgataac aacgataaag aggtgcacga tcagcatcac cagatgcagc
     1861 aacgccgtat agccggcgtg atgcaggccc agcagcagcg ccacattcgc aaaggcgatg
     1921 cctgacacgc cgacggcgag gatgcgccgc gcccagatct cgatataggc cgcggtctcg
     1981 gcgcgaacgc gaaacagacc gaaaggtccc gccaacgccc gcacgacaca gattagcccg
     2041 cgcgacagcg cgtaggcatt cacgaccgcg aggatcacca gccgggcggt tggggcatcg
     2101 ccgatctcgg aacccaacaa catcgtggcg acaccgacga agacgagcac cgggagcagc
     2161 tcgagcgcga gacgtcccaa cacgaacggc agtctgacca gcgattgcca ggcgcgcgca
     2221 agactgagac gccgttggtg cagttcggac tcagcggtta gatccgcc
//
LOCUS       DATMRM010000457         5660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3038914, whole genome shotgun sequence.
ACCESSION   DATMRM010000457 DATMRM010000000
VERSION     DATMRM010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3038914"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VNZ06_13860"
     CDS             <1..355
                     /locus_tag="VNZ06_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21880.1"
                     /translation="SVVEVAPSAVPGMHKVATMVIAPSADLRVIATRHVRSLPRGLRS
                     LLRVMGARGSAGAQLASYLMFESSFTRELIQLGYQDAMNRREDLLGFLAGAPLAATTI
                     LPALELADVADQLPT"
     gene            360..707
                     /locus_tag="VNZ06_13865"
     CDS             360..707
                     /locus_tag="VNZ06_13865"
                     /inference="COORDINATES: protein motif:HMM:NF005297.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor Sui1"
                     /protein_id="HWW21881.1"
                     /translation="MSIVYRTGVGRICPGCRRPIADCVCKSATGQSLRVHAGAVRVAR
                     QTQGRAGKAVTVVTGLPLDQPELEALATQLKRRCGSGGTVRQGVIEIQGEHRDTVVAE
                     LIRRGYAAKRSGG"
     gene            complement(713..1609)
                     /gene="rapZ"
                     /locus_tag="VNZ06_13870"
     CDS             complement(713..1609)
                     /gene="rapZ"
                     /locus_tag="VNZ06_13870"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HWW21882.1"
                     /translation="MRLIFVSGLSGSGKSVALHMLEDLDFYCVDNIPAALLKAFISHT
                     VRCDSNVYQRTAIGIDARNPGAEISTVPMLLDELKRSGIHCELVFLVASDEELLRRFA
                     ETRRKHPLSRPGESLHEAIAGERDLLEPLIQAADLVVDTSRMGVHDLRELIDRRVGRR
                     PRDRMSVLFESFGFKHGIPGDADFVFDARSLPNPYWHPTLRPLTGRDDAVAHFLAGQP
                     AVARFIDEVATFIEARIPEHQAASRRYLTIAIGCTGGQHRSVYIVEQLAARVVKLWAQ
                     VAVRHSALPELAKGSPAQDVAS"
     gene            complement(1655..2023)
                     /gene="raiA"
                     /locus_tag="VNZ06_13875"
     CDS             complement(1655..2023)
                     /gene="raiA"
                     /locus_tag="VNZ06_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HWW21883.1"
                     /translation="MNAGESTESDFQGEHAMHLNVTGHHVDVTPPLRSYVEKKLGRIM
                     RHFDHLIDSHFVLTVEKLQHKAEATLHVRGETIHAVAIEADMYASIDALADRLERRVR
                     KHKEKLCDHHAQEAQKSARL"
     gene            complement(1995..3431)
                     /locus_tag="VNZ06_13880"
     CDS             complement(1995..3431)
                     /locus_tag="VNZ06_13880"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HWW21884.1"
                     /translation="MLKPTLQLRLGQQLTMTPQLQQAIRLLQLPTIELQAHIRELLES
                     NVMLEQDEDTESGIYEQLPAAETAPETVARAEEPPVEVVDENWADQSVGPAENPWNNG
                     GSSDEDRQQEVADSSGQSLRDHLQWQLELAKLNARDLSIGRAVVDAINDDGYLSEPLD
                     VIGASLKPELDPGVEEVERVLMIIQMFDPAGVGARSVSECLQLQLEQLGPDTPGLATA
                     KAIARDHLELLADRELTALRRELRASDEELETAVALVRSCNPRPGSIISGSAAEYVVP
                     DVFVRRTPRGWSVEINAATLPRVRLNQSYANLVGRSSSHASMRTQLQEARWLLKSLEI
                     RNDTLTKVARSIVERQSEFLEHGEEFMRPMILRDIAEAIEMHESTVSRVTSGKYMHTP
                     RGVFELRYFFSSQVEGSDGSGTSSTAIRAKIRKLIREEDPTAPLSDSRIAEILSGEGI
                     PVARRTVAKYRETMGLAPSNERRRVNGK"
     gene            complement(3516..4241)
                     /gene="lptB"
                     /locus_tag="VNZ06_13885"
     CDS             complement(3516..4241)
                     /gene="lptB"
                     /locus_tag="VNZ06_13885"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005096670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HWW21885.1"
                     /translation="MTLLAARSLQKSYRSRQVVRDLSIEIHTGEVVGLLGPNGAGKTT
                     AFYMIVGLIPADGGSIFLDDRNLTALSIDQRARLGLGYLPQEASVFRRLTVEDNVMAI
                     LELRDELDRAGRERRLEELLSELRIAQVRRTRGMALSGGERRRVEIARALAAEPRFIL
                     LDEPFAGVDPLSVLDIQRIIRELAERGIGVLITDHNVRETLGVCGRAYIVDRGVVIAS
                     GSAEQVLANPQVREVYLGQAFRL"
     gene            complement(4238..4924)
                     /locus_tag="VNZ06_13890"
     CDS             complement(4238..4924)
                     /locus_tag="VNZ06_13890"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HWW21886.1"
                     /translation="MRPRPSAVVWLLLAAAEVHGAATPPVDYKALLRSCREEVCLNGS
                     HLEATLTQTRQTWHDITIVYSARSAVVVGDLGEGEVSSRDSKNSHWTVSGHVQVFTLE
                     GHLSADRATVQIVNGRVTTVTAEGAPAQFERSPDTASPANLSPSAQAALEHAHGHAHE
                     IVFDVDRSQLEFSGDAYFTAGCSEFSSEHVVYDMANQKIEGDPRDHSFVSGTYKSGTS
                     GCGGPAAHKP"
     gene            complement(4932..5492)
                     /gene="lptC"
                     /locus_tag="VNZ06_13895"
     CDS             complement(4932..5492)
                     /gene="lptC"
                     /locus_tag="VNZ06_13895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HWW21887.1"
                     /translation="MIWRILFALVSLTALGALLYLRQSDSANDVSIVQELPPELGYVA
                     THGDLVETGDNGHPLYRLDADRIEQPVPHGMIYLSSPRLDYQSEPDDHWILTAQKGEL
                     PQDAQYADLSGTVHVEGRPTGSNDLMRIDTDALHLDMPQQIATTPVKVTARWNGMTLS
                     GHGMRYEMKRNALELQAEVHGALGQR"
BASE COUNT         1003 a         1804 c         1782 g         1071 t
ORIGIN      
        1 cagtgtggtc gaagtggcac cgagcgcggt gccggggatg cacaaagtcg caacgatggt
       61 gatcgcgcca agcgcggacc tgcgggtgat cgcgacccga catgtgcgca gcctgccacg
      121 cggcctgcgt tcactgctgc gtgtgatggg tgcgcgcggt tccgctggcg cccagctggc
      181 cagctacctg atgttcgagt catcgtttac gcgtgagctg atccagctcg gctatcagga
      241 cgcgatgaac cggcgcgagg atctgttggg atttctggca ggagcgccgc tggcggccac
      301 cacgatactg ccggccttgg agctggccga cgtcgctgac caactgccga cctgaagaag
      361 tgagcatcgt ctatcgcacc ggtgtcggcc ggatctgtcc cgggtgccgt cgtccgattg
      421 ccgactgcgt ttgcaagagc gccacggggc agtcgctgcg tgtgcacgcc ggcgccgtgc
      481 gggtcgcacg ccagacgcag ggccgcgccg gtaaagcggt caccgtcgtg accggcctgc
      541 cgctggatca gccggaactg gaggcgctcg ccacgcagct caaacgtcgc tgcggcagcg
      601 gcggtaccgt caggcaaggc gtgatcgaaa ttcagggtga gcaccgcgac acggtggtcg
      661 ctgaactgat ccgtcgcggg tatgcggcca aacgctccgg cggctgagct cttcagctcg
      721 ctacgtcctg cgccggtgat cccttcgcaa gctcgggcaa cgcggagtgt cgcacggcca
      781 cttgcgccca gagcttgacc actctggccg cgagctgctc gacgatataa accgagcggt
      841 gctggccacc ggtacagccg attgcgatcg tcagataccg ccggctggcg gcctgatgtt
      901 ccgggatgcg cgcctcgatg aaggtcgcaa cctcgtctat gaaacgcgcc acggcgggtt
      961 gtccggcgag aaaatgagcc accgcgtcat cgcgtccggt cagcggccgc agggtcggat
     1021 gccagtacgg attgggcagc gagcgcgcgt cgaacacgaa gtcggcgtct ccggggatgc
     1081 cgtgtttgaa accgaaggac tcgaacagga cggacatgcg atcgcgaggt cggcggccga
     1141 cccgacgatc gatcaattcg cgcaggtcat gcacgcccat gcgcgaggta tcgactacca
     1201 gatccgcggc ctgaatcagc ggttcgagca gatcgcgttc accggcaatc gcctcgtgca
     1261 ggctttcgcc aggacggctc aagggatgtt tgcgccgtgt ctcggcgaaa cgccgtaaca
     1321 gttcctcatc gctggcaacc agaaacacca gttcgcaatg aatgccgctg cgcttgagct
     1381 catccaatag catcggcacg gtgctgattt ccgcgccggg attgcgagcg tcaatcccga
     1441 tcgccgtgcg ctgataaacg ttggagtcgc agcgcacggt atgtgagatg aaggctttga
     1501 gcagagcggc cggaatattg tcgacgcagt agaaatccag gtcctcgagc atgtgcagcg
     1561 ccacgctctt gccggaaccc gacagaccac tgacgaaaat cagccgcacg gcgcccccct
     1621 gtccgacaac gccgtggctg agtgccgctg ggggctagag tcgggcgctt ttctgggctt
     1681 cctgagcatg gtggtcgcag agcttttcct tgtgcttgcg cacacggcgc tcgagtctgt
     1741 cggccaatgc atcgatgctg gcatacatat ccgcctcgat ggccacggca tgaatcgtct
     1801 caccgcgcac gtgcaacgtc gcttccgcct tgtgctgcaa cttttccact gtcagaacga
     1861 agtggctgtc aatcaggtga tcgaaatggc gcatgatgcg gccgagtttt ttctccacgt
     1921 aactgcgcag cggcggggtg acgtcgacgt gatggccggt gacgttcaaa tgcatggcat
     1981 gttctccttg gaaatcactt tccgttgact cgcctgcgtt cattggatgg tgccagtccc
     2041 atcgtttccc ggtacttagc tacggtccgg cgcgcgaccg ggatgccctc gccagacaaa
     2101 atttctgcaa tccgactatc gctcaacggc gcggtcggat cctcctctcg tatcagtttg
     2161 cggattttgg cgcgaatggc cgtggaagaa gtgcccgagc cgtcgctgcc ttcgacctgg
     2221 ctcgagaaga agtagcgcag ctcgaacacg ccgcgcggtg tgtgcatgta cttgcccgag
     2281 gtgacgcgcg agacggtgga ctcgtgcatc tcgatcgctt cggcgatatc gcgcaggatc
     2341 atcggccgca tgaattcttc accgtgctcc aggaattcac tctgacgctc gacgattgac
     2401 cgcgccactt tggtgagcgt gtcgttgcgt atctccagac tcttgagcag ccagcgcgct
     2461 tcctgcagct gggtgcgcat gctggcatgg ctgctgctgc ggccgactag attggcatag
     2521 gactgattga gccgcacgcg tggcaaggtg gcggcgttga tctccaccga ccagccgcgc
     2581 ggtgttcgcc gcacgaacac atccggcacc acgtattcgg ccgcactgcc gctgatgatc
     2641 gagccgggtc gggggttgca tgagcgcacc agtgcgaccg cggtctccag ctcctcatcc
     2701 gaagcgcgca gctcccggcg cagcgccgtg agctcgcgat cagcgagcag ttcaagatga
     2761 tcgcgtgcga tggctttcgc cgtggcaaga cccggggtat ccggcccaag ctgctcgagc
     2821 tgcagttgca ggcattcgct caccgagcgg gcgcccacgc ccgcgggatc gaacatctga
     2881 atgatcatca gcacgcgctc gacttcctcg accccaggat cgagttccgg cttgaggctt
     2941 gccccaatca catccagcgg ttcgcttaag tagccgtcgt cattgatcgc atcgacgacg
     3001 gcgcgcccga tcgacaggtc acgggcattg agtttggcga gctcgagctg ccactgcaga
     3061 tgatcgcgca gcgactggcc gctcgagtcg gcgacctctt gctggcgatc ctcgtcgctg
     3121 ctgccgccat tgttccaggg attctcagcc ggcccgaccg actgatcggc ccagttctca
     3181 tccaccacct cgaccggcgg ttcttccgct cgggcgacgg tttccggtgc ggtttcagcc
     3241 gccggcaact gctcgtagat accgctttcc gtgtcttcgt cctgctcgag catcacgttg
     3301 gactcgagca gctcgcgaat gtgcgcctgc aattcgatgg tcggcagttg tagcagcctg
     3361 atcgcctgct gcagctgcgg agtcatcgtc agctgctgtc caagtcttag ctgcagggtc
     3421 ggcttaagca tgaatcatgc ccgaaggcgc ctaggtcgcg gcggatacat tcataaccat
     3481 ctgataatta agcaataaga tatcgacgat gaccgctaca ggcggaatgc ctgacccaag
     3541 tacacctcac gcacttgggg gttggcaagg acttgctcgg cagacccgga agcgatgaca
     3601 accccccggt ccacgatata ggcgcggccg cagaccccta aggtttcgcg cacattatgg
     3661 tcggtgatca gcacgccgat gccgcgttca gccagttcac gaatgatgcg ttgaatatcc
     3721 agtaccgaga gcggatccac accggcgaaa ggctcatcca gcaggatgaa acgcggttcc
     3781 gccgcgaggg cgcgtgcgat ctcaacacgg cggcgctcac cgccggacag cgccatgccg
     3841 cgggtgcggc gcacctgcgc aatgcgaagc tcggacaaca gctcctccag ccgacgttcc
     3901 cggccggcgc ggtccaactc atcgcgcagt tccaggatcg ccatgacgtt gtcctcgacc
     3961 gtcaggcgcc ggaataccga ggcctcctgc ggcaaatagc ccagacccag gcgcgcgcgc
     4021 tgatcgatgc tcagcgcagt caggttgcgg tcatccagga agatcgaacc gccatcggcc
     4081 ggtatcaggc cgacgatcat gtagaacgcc gtggtcttgc cggcgccgtt cggtcccagc
     4141 aggccaacaa cctcacccgt gtggatctcg atcgacagat cgcgcaccac ctgacgcgag
     4201 cggtagcttt tctgcagtga gcgcgctgcc agcagtgtca tggcttgtgc gcagctgggc
     4261 cgccgcatcc cgaggtgccg gatttgtacg taccgctgac gaaggagtgg tcgcgcggat
     4321 cgccctctat cttctgattg gccatgtcgt acacaacgtg ttcgctgctg aattctgaac
     4381 agcctgcggt gaaataggcg tcgccactga attcgagctg actgcggtca acgtcaaaca
     4441 caatttcgtg cgcatgcccg tgggcgtgct cgagtgccgc ctgcgcactg ggggaaaggt
     4501 ttgccggcga cgcggtgtcg ggtgagcgct caaattgcgc cggggccccc tctgcggtca
     4561 ccgtggtcac tcgaccgttg acgatctgga ccgtcgcccg gtctgcgctc aggtggccct
     4621 ctagtgtgaa cacctgtaca tgcccggaca cggtccagtg gctgttcttc gagtcgcggc
     4681 tgctgacctc gccctcgccc agatccccta ccacaaccgc cgagcgtgcc gagtacacga
     4741 tcgtgatgtc gtgccaggtc tgacgcgtct gcgtcagtgt tgcctcaagg tgggaaccat
     4801 tcaggcacac ctcttcacgg cagctgcgca gcagagcttt gtagtccacc gggggcgtcg
     4861 ccgcgccatg aacctctgcc gcggccagca gcagccatac cacagcgctt gggcgtggcc
     4921 tcatggcgtg ctcagcgctg acccaacgca ccgtgcacct ccgcttgcag ctcaagtgca
     4981 ttgcgcttca tttcgtagcg catgccgtgg ccggaaaggg tcatgccgtt ccaacgggca
     5041 gtcaccttca cgggggtggt ggcgatctgc tgcggcatgt ccagatgcaa cgcatcggtg
     5101 tcgatgcgca tgagatcgtt ggaacccgtg ggtcggccct cgacatgcac cgtgccgctc
     5161 aggtcggcgt actgcgcgtc ctgcggcagc tctcctttct gcgccgtcag tatccagtga
     5221 tcgtcaggtt ccgactgata gtccagccgt ggcgaggaca ggtagatcat gccgtggggg
     5281 acaggctgct cgatgcgatc ggcatccagc cggtagagcg ggtggccatt atcgcccgtt
     5341 tcgaccaggt cgccgtgtgt ggcaacgtag cccagctccg ggggtagctc ctgcacgatg
     5401 ctgacatcgt tggcgctgtc gctctgacgc aggtacagca gcgccccgag cgcggtcagc
     5461 gacaccagcg caaacagaat gcgccagatc atgcgcgccg cgcctgcagc agccagtcac
     5521 agacttcacg gaccgcaccc gcgccacccg gcagacgggt gcgacggtgc gcactcgagg
     5581 cggcgcaggg gtgtgcgtcg gcgacggcaa ctgccagacc cgcgagctgc aacagcggca
     5641 catccggttc gtcatcgccc
//
LOCUS       DATMRM010000458         8426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3656357, whole genome shotgun sequence.
ACCESSION   DATMRM010000458 DATMRM010000000
VERSION     DATMRM010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3656357"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2526)
                     /gene="cofG"
                     /locus_tag="VNZ06_13900"
     CDS             complement(<1..2526)
                     /gene="cofG"
                     /locus_tag="VNZ06_13900"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HWW21888.1"
                     /translation="MKIWAVMPVKALQTSKQRLATALGIRRERFVRALLHQTLFALAH
                     SRRVSGILVVTNDAKVAAEAREAGAEVCQEDADLNAACVLGLGEVRTRGCEVCVIIHA
                     DLAWLSPQGVDALVNAYLEHRRVQGEGVIGLVRCKEGTGTNAVLLDPRLPFEPAFGPA
                     SFAAHQLGLGARAQELRSDEVAFDVDTATDLQTLAAGPVPARLRPFVAGPFAAGPLVV
                     GNESQLDPMSLIDLPPEKLLVQAARLRDEGHDALLTYSRKVFLPLTQLCRDSCHYCTF
                     AQSPRRAGRPYMSVEAVLTVATAGAERGCKEALFTLGEKPELRYQVARSWLSEAGFES
                     TLHYVAHLAAAVRDKTGLLPHINAGCMTAQEMAMLRPVSASMGLMLESASERLCAKGG
                     PHYGSPDKAPALRLATIAEAGRQQVPFTTGILIGIGETRAERIESLLAIRALHEQFGH
                     IQEIIIQNFLPKVGTRMAKAAAPSIDELLWTVAVARILFGPLMNIQAPPNLNPGALGR
                     LVEAGVNDWGGVSPLTPDFVNPEAPWPQIERLRADTAAAGKILAERLTIYPSYAQAPQ
                     MWLDPAMQRSVLELRDGSGRAREDAWRAGRSTNLPPSPVTGRLTASARLSRVLGDIVD
                     HGADWIGVDEIARLFEARDADFAAVCATADALRAAAVGDVATYVVNRNINYTNMCTYH
                     CGFCAFSKGTRKHEGAEKPYLFDVREIEARVREAWERGATEVCLQGGIHPSFTGETYI
                     DIVRAVKRGAPGMHVHAFSPLEVWHGASTLKLGLRAYLQRLRDEGLSSLPGTAAEILD
                     DGVREVLCPDKINTSQWLKVIETAHNIGLRTTATIMYGHVDDYR"
     gene            complement(2544..3212)
                     /gene="npdG"
                     /locus_tag="VNZ06_13905"
     CDS             complement(2544..3212)
                     /gene="npdG"
                     /locus_tag="VNZ06_13905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HWW21889.1"
                     /translation="MNDLTIAVLGGSGKEGSGLALRWAKAGHPVILGSRSADKAQASA
                     AELNQILGAIHVQFADNATAAAKADVVVLAVPYSAQQSTALGIRDRLVGKILIDVTVP
                     LKPPKVSMVNLPAEGSAVVALQRALGPQVRVVSAFQNVSAAHLRDLDHSVDCDVLICT
                     DDEAAGEIAVALAQSAGMRGWRCGPLANSVVSEGLTSVLIAINQRYKIPGSGIRITGT
                     PKAT"
     gene            complement(3209..4357)
                     /locus_tag="VNZ06_13910"
     CDS             complement(3209..4357)
                     /locus_tag="VNZ06_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21890.1"
                     /translation="MFKDYNPATPLHRADAFRFLTQNLGQAFDLALETKDTHFPVIHA
                     FVTPFCKLGGDCADFVYQQAWIDGESVYKISGNRGSARFLNITVQGPRPDKQPGTDWP
                     SLNEPFGDIPEANLFGHQLETAWDGSFELFIGGPRRTPNWLPTTPGSRKLFMRQGFDR
                     WSELPARLRIERVGMAEPRPLPGPQQLVTAIDWAGHFITGLMDDWPDHPYQYSGGVVD
                     AVHVNVFPPEPKGDAASDKRRGRMIANLCWQLAPDEALIVEFNSHDGFWMMTNMGVFF
                     NSMDFLYRPVSYTPSRATIDSDGKIRLILCHEDPGYHNWLDTQGFVRGNLTYRNLMSQ
                     AQTPFRTELVKRSRLVDALPADSVKTSPEDRRCQMHLRFDAIRQRYSL"
     gene            complement(4460..5275)
                     /locus_tag="VNZ06_13915"
     CDS             complement(4460..5275)
                     /locus_tag="VNZ06_13915"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HWW21891.1"
                     /translation="MHPRISVNTLCFPGASVGDMAGHWRELGVRRVSLVSNLLLEEGV
                     PAAQEALRTGDYQVETISHAFMFGRHLELREESWREARDTLARVIEAAKIIGARSIYM
                     VTGGHGSLLWEEAAQAFRAAIAPCIAQASAAGIPLAIEDASALYAHANLAHTLRDTVV
                     LAEMADVGVCIDFFACWTEAGLQQSIERAAPRCRIVQVCDYVYGDRSLPARAVPGDGN
                     IPIKRMLDWILKAGYTGAFDLELIGPRIDREGHLLAVRRAAENLGAMLQSLGA"
     gene            complement(5291..6460)
                     /locus_tag="VNZ06_13920"
     CDS             complement(5291..6460)
                     /locus_tag="VNZ06_13920"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWW21892.1"
                     /translation="MASGNDTLNADSLLAQAEAQTGLRDYGDDTLPARFRLAVGYLQS
                     VRMDDAGQRAAASVCLWLLTSRLELLQDRRRYPIAQEKIERPLFATGEPRSGTTLLHA
                     LLSLDPNGRALRFWEVMYPSPPPGLAEPDDVRRARADEDWREILRKLPKWLISHPYND
                     MLGNGLPECERTWAFDFRVMTPTAWWRVPMGMNIGGLPTDPLAQYRLHKMMLQQCQYA
                     RPQKYWVLKGFHALRLPALFETYPDARIIWVHRDPVQVTASRIVLTGELVEGLAGHVD
                     WKEQARLHLAATRAGFQSTLNNPMIDDPRIHHVRYPDFMADPVGTIGGFYKKCGVPFN
                     SDTEAAMHAYLRNNKGDRYGKFRYSTDIIGTDIQALHAEFEPYRKRFGLDIEQRK"
     gene            complement(6464..7582)
                     /locus_tag="VNZ06_13925"
     CDS             complement(6464..7582)
                     /locus_tag="VNZ06_13925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWW21893.1"
                     /translation="MNVQPAAFVRTTLPLDLSKLDALDSGRYHSVWLPDHMVSFWPDA
                     IWTAEFTDLATTSASPHRHLDGLAVAAAVAALTKNVTIATSVVDTVRRHPSLLAQSAL
                     TIDHLSRGRFILGVGSGETENTVPYGFDFAKPVARFEEALQVIQLLWKSEGPVDFSGQ
                     FYKLRHARLDTEPYEGRVPRIWAGGSGPRMLDIIGRYCDGWWPAGAYSPEDYAAKLKV
                     VRDSATRAGRDPMTITPAFIWTCLIADNDAELAKIVEAPLVKAYVLQIPAAIMRQYGF
                     EHPLGDQWRGYQDINPEVLPRERILAMLKKVNVEAILAVVPHGTPQQVARTLKGFVEA
                     GLRVPKILDYGGMAGLQYAARSAQKVRDAEDELMRLVG"
     gene            complement(7675..>8426)
                     /locus_tag="VNZ06_13930"
     CDS             complement(7675..>8426)
                     /locus_tag="VNZ06_13930"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HWW21894.1"
                     /translation="SVAQGWGREGESWSFMEAMRSLGGPHWFALGDGDLALHVLRTQR
                     LRSGEALSAIIADFATAWRIKAKILPMSNDPVATRVQAEEGELEFQQYFVARKCEPVV
                     KAIRFHGAASAQAAPGVLEALRDAHTRAILIAPSNPFLSIDPILAVPSIRQALMQTDV
                     PVIAVSPLVGGTAVKGPTAKLMRELGIEVSATALVRHYGELIDGMLIDERDGPAAFEL
                     AQARADTLMKTLDDRIRVARAALELADSIRT"
BASE COUNT         1628 a         2696 c         2489 g         1613 t
ORIGIN      
        1 tcggtagtcg tcgacgtgcc cgtacatgat agtggccgtg gtgcgcaaac cgatgttgtg
       61 cgcggtctcg atgactttca accactgact ggtgttgatc ttgtcgggac acagcacttc
      121 gcgcacgccg tcatccagta tctccgccgc ggtgcccggc agactgctca gcccttcgtc
      181 ccgcagacgc tgcagatagg cacgcaaacc cagcttcagg gtgctggcgc catgccagac
      241 ttccagcggc gaaaatgcat gtacatgcat gcccggcgcg cctcgcttca ccgcacgcac
      301 gatatcgata taggtctcac cggtaaaact tggatgtatg ccaccctgaa ggcacacttc
      361 ggtggcgccc cgctcccacg cttcgcgaac gcgtgcctca atctcccgga catcaaacag
      421 atagggcttc tccgctccct catgcttacg cgtgcccttc gagaaagcgc agaaaccgca
      481 gtgataagta cacatattgg tgtagttgat gttgcggttc acgacgtacg ttgcgacatc
      541 tcccaccgcc gccgcccgca gtgcgtcggc ggtcgcgcag acggctgcga agtcagcgtc
      601 gcgcgcctcg aacaggcgcg cgatctcatc caccccgatc caatccgcac cgtgatcgac
      661 gatgtcaccg agcactctcg atagccgcgc cgaggccgta agccgacccg tcacgggcga
      721 tggcggcagg ttggtcgagc gtccggcccg ccaggcatcc tcccgggcgc ggccactgcc
      781 atcgcgcagt tccagcaccg accgttgcat ggccggatca agccacatct gcggagcttg
      841 cgcgtaggac ggataaatcg tcaggcgctc agccagaatt tttcctgccg ctgcggtatc
      901 cgcgcgcaaa cgctcgatct ggggccatgg ggcctctgga ttcacaaagt cgggagtcag
      961 cggcgagacg ccgccccaat cattcacacc ggcttccacc aggcggccca gtgcgcccgg
     1021 attgaggttc ggtggcgcct ggatgttcat gagcgggccg aacagaatgc gcgccactgc
     1081 caccgtccac agcagttcgt cgatcgatgg cgcggcggct ttggccatgc gcgtgccgac
     1141 tttcggcaga aaattctgga tgatgatttc ctggatgtga ccaaattgct catgcaatgc
     1201 acgaatcgcc agcaatgact caatgcgctc ggcccgtgtc tcgccgatgc cgatcaggat
     1261 accggtggtg aatggcacct gctgacgccc agcctcggca atcgtcgcca gcctcagcgc
     1321 tggcgctttg tcgggagaac cgtagtgcgg gccacctttt gcacacagtc gttctgaagc
     1381 gctttccagc atcagcccca tcgaagccga caccgggcgt aacatcgcca tttcctgcgc
     1441 agtcatgcac cctgcgttga tgtgaggcag cagcccggtc ttgtcgcgca ccgcggccgc
     1501 gaggtgcgcg acatagtgca gcgtgctctc gaagcccgcc tccgacagcc atgatcgcgc
     1561 gacctggtag cgcaactcgg gtttttcgcc caacgtaaag agagcttcct tgcaaccgcg
     1621 ttcagcccct gcggtcgcca ccgtcagcac ggcctctacc gacatgtacg gccggccggc
     1681 gcgccgcggc gattgggcga aggtgcagta gtgacaactg tcgcggcaaa gctgcgtcag
     1741 cggtagaaat actttgcgcg agtaagtgag cagcgcatcg tggccttcat cgcgcagacg
     1801 ggcggcctgc accagcagct tctccggtgg cagatcgatg agagacatcg gatccagctg
     1861 cgattcattc ccaacgacta acgggccagc ggcgaatggg ccagctacga acgggcgcag
     1921 ccgcgctggt accgggcctg cagccagagt ttgcaaatcg gtggcggtgt cgacgtcgaa
     1981 ggctacctca tcggaacgca gttcctgggc acgcgcaccg aggccaagct ggtgggccgc
     2041 gaagctggcg gggccgaacg ctggctcaaa cggcagtctg ggatcgagca gcacagcgtt
     2101 ggtaccggtg ccttccttgc agcgaaccaa tccaatgacg ccctctccct gcacgcgccg
     2161 atgttccaga taggcgttca ccagggcatc gacaccttgc ggcgacagcc atgcgagatc
     2221 cgcgtggatg atcacacaca cctcgcaccc tcgagttcgc acttctccta agccgagtac
     2281 gcaggccgca ttgagatccg catcctcctg gcagacttcg gcgcccgcct cgcgcgcttc
     2341 ggcagcgacc ttcgcatcgt tggtcaccac caggattccg ctcacgcgtc gcgagtgcgc
     2401 aagggcgaac aatgtctgat gcaacagggc gcgaacgaag cgttcccggc ggattcccaa
     2461 cgcagtggct aacctttgtt tcgaggtctg cagcgctttg accggcatca ccgcccaaat
     2521 tttcaacgcc tgcgccggca tcttcaggtg gccttcggtg tgccggtgat ccggatgccg
     2581 gaaccgggga ttttgtagcg ctggttgatc gcgatcagca cagaggtcaa gccttcgctg
     2641 accacggaat tggccagagg cccgcagcgc cagccgcgca tcccggccga ctgcgccaga
     2701 gccacggcga tctcgcccgc cgcctcatca tcggtgcaga tcagcacgtc gcaatccacc
     2761 gaatgatcga gatcgcgcaa atgcgcagcg gagacgttct gaaacgccga cacgactctg
     2821 acctgcggtc cgagcgcacg ctgcaaggcc acgaccgcgg acccttcggc gggcagattg
     2881 accatgctga cctttggcgg tttcagcggg acggtgacat caatcagaat ctttccgacc
     2941 agccgatcac gaatccccag cgcggtcgat tgttgcgcgc tgtagggaac ggccagcacc
     3001 accacatcgg ccttcgccgc agcggtggcg ttgtcggcga actgaacgtg aatggcgcca
     3061 agaatctggt tgagctccgc agcgcttgcc tgcgccttgt ctgcgctgcg actacccagt
     3121 atcacggggt gccccgcctt ggcccaccgc aatgcaaggc cgctgccttc cttgccgctg
     3181 ccaccgagta ccgcgatcgt gagatcgttc atagactata gcgttgacgt atcgcgtcga
     3241 agcgcaggtg catctgacac cgtctgtcct ccggtgatgt cttgacgcta tcggcgggca
     3301 gggcgtcaac aagccgcgag cgcttgacca gctcggtgcg aaacggcgtc tgcgcctggc
     3361 tcatcagatt ccgataggtc agattacctc gtacgaaccc ctgagtgtcc agccaattgt
     3421 gatagccggg atcctcatgg cagaggatca acctgatctt gccatcgctg tcgatcgtgg
     3481 cacggctggg ggtgtagctc accggccgat agagaaagtc catgctgtta aagaaaactc
     3541 ccatattcgt catcatccaa aagccgtcgt gactgttgaa ctccacgatc aatgcttcgt
     3601 cgggagccaa ctgccaacac aggttggcga tcatacggcc acgtcgcttg tctgaagccg
     3661 catcaccctt tggctctgga gggaagacat tgacgtggac ggcgtcgacc acaccgccac
     3721 tgtactggta gggatgatcg ggccagtcgt ccatcaatcc ggttatgaag tgacccgccc
     3781 aatcgatcgc ggtcactagt tgctggggcc caggcagtgg tcgcggttcg gccataccca
     3841 cgcgctcgat gcgcaagcgc gccggtagct cgctccagcg atcaaatccc tggcgcatga
     3901 acagcttgcg cgaaccaggc gtcgtcggca gccaattcgg agtgcgtcgc ggaccgccga
     3961 tgaaaagttc aaagctcccg tcccaggccg tttcgagctg atggccgaac aggttggctt
     4021 cgggaatatc gccgaaaggc tcattgaggc ttggccaatc ggttcctggt tgcttgtcgg
     4081 gccgcggacc ttgaaccgtg atgttcagaa agcgcgcgct acctctgttg ccggaaatct
     4141 tgtagaccga ttcgccgtca atccaagcct gctgataaac aaaatcggcg cagtcgccgc
     4201 cgagtttgca gaatggagtc acgaaagcat gtatgacggg aaaatgcgta tccttcgttt
     4261 caagcgccag atcgaacgcc tgtcccaggt tctgcgtcag aaagcggaag gcatccgctc
     4321 gatgtagcgg tgtcgccgga ttatagtctt taaacactcg gtcgcctgcc ttctgtaggc
     4381 gctcacagaa gacgccccag gcagcgcgca atgactcgtc gtcgcgacaa tctccaaacg
     4441 ccatcgactt gctctcccgt caggctccga gcgattgcag catcgcgcca aggttttccg
     4501 ctgccctgcg aacagcgagc agatgtccct ccctatcgat acgcggcccg atcaactcga
     4561 ggtcgaaagc accggtgtac cccgctttca gtatccagtc gagcattcgc ttgatcggaa
     4621 tgttgccgtc tccgggcacc gcccgcgctg gcaaagatcg atcgccatag acatagtcgc
     4681 agacttgtac gatacggcag cgcggcgctg cgcgctcgat ggattgttgt agtcccgcct
     4741 cggtccaaca ggcaaagaaa tcgatgcaca cgcccacatc cgccatttcg gcgagcacca
     4801 cggtgtctcg caacgtatgg gccagattcg cgtgggcata gagcgccgag gcatcctcga
     4861 ttgccaacgg gattccggcc gcgctggcct gcgcaatgca gggtgcgatc gccgctcgga
     4921 aggcttgcgc agcttcctcc cagagcagag aaccatgacc gccggtcacc atgtagatag
     4981 acctcgcacc gatgatcttt gcggcctcga tcacgcgcgc cagcgtgtca cgggcttcgc
     5041 gccagctctc ttcgcgcaac tccagatgcc ttccgaacat gaaggcgtga gaaatggttt
     5101 cgacctggta atcaccggtt cgcagtgcct cctgcgcagc cggtacgccc tcctccaaca
     5161 gcaggttgct caccaggctg acgcggcgca cgccaagctc ccgccagtga ccggccatat
     5221 cgcccacact ggcgcccgga aagcacagtg tattgacgga tattctggga tgcatgtgcg
     5281 cccgcgccac ttacttgcgc tgttcgatat ccagaccaaa acgcttgcga tagggctcga
     5341 actccgcgtg cagcgcctgg atatcggtgc cgatgatgtc ggtcgaataa cggaacttgc
     5401 cgtaccgatc gcctttgttg tttcgcagat aggcgtgcat ggcagcctca gtgtcggagt
     5461 tgaacggtac accgcacttt ttgtagaaac caccgatcgt gccgacggga tcggccatga
     5521 agtccggata tcggacgtgg tgtatgcgcg gatcgtcgat catgggattg ttcaaggtcg
     5581 actggaagcc tgcgcgggtc gcggccaggt gcaggcgtgc ctgctccttc cagtccacat
     5641 gaccggcgag cccctcgacc agttcgccgg tcagtacgat gcgcgacgcg gtgacctgaa
     5701 ctggatcgcg gtgcacccaa ataattctcg cgtccgggta ggtctcaaac agcgccggca
     5761 gccgcaaggc gtgaaagccc ttcagcaccc agtatttctg aggtctcgcg tactgacatt
     5821 gctgcaacat cattttgtgc agacgatact gagcaagcgg atcggtcggc aggccgccga
     5881 tattcatgcc catcggtacg cgccaccagg cggtcggcgt catcacgcgg aaatcgaatg
     5941 cccaggtgcg ctcacattcc ggcaagccgt tgccgagcat gtcgttgtaa ggatggctga
     6001 tcagccactt gggcaatttc ctgaggatct ctcgccagtc ctcgtccgcg cgtgcgcgcc
     6061 gcacatcgtc tggctctgcg aggccaggcg gtggcgaggg atacatgacc tcccaaaacc
     6121 gcaacgcgcg cccattggga tcgagcgaca gcagcgcgtg cagcaaggtc gtgccggaac
     6181 gcggctcgcc ggttgcgaac agtggccgct cgattttctc ctgtgcgatg gggtatcgcc
     6241 tgcggtcttg cagtaactcc aggcgcgagg ttagaagcca gaggcacacc gacgcggcgg
     6301 cacgttgacc ggcatcatcc atccgcacgc tctgtaggta gcccaccgcc aggcgaaatc
     6361 tggccggtag cgtgtcgtcg ccatagtcgc gcagaccggt ctgcgcctcg gcctgagcca
     6421 gcagactatc cgcgttcaaa gtatcgttgc cggaagccat gattcatccc acgagacgca
     6481 tcagttcatc ctcggcgtcc cgcaccttct gcgctgatcg tgccgcgtat tgcagacctg
     6541 ccatcccgcc gtaatcgagt attttgggga cccgcagtcc ggcttccacg aagcccttga
     6601 gcgtgcgggc gacctgttgt ggtgtgccat gcggcacgac tgcaagaatt gcttcgacgt
     6661 tgaccttctt cagcatcgcc agtatgcgct cgcgcggcag aacttcggga ttgatgtcct
     6721 gatagccacg ccattggtcg cccagaggat gttcgaagcc atattggcgc atgatcgccg
     6781 caggtatctg caacacgtag gccttgacca gcggtgcctc gacaatcttc gccaattccg
     6841 catcgttgtc ggcgatcaga caagtccaga tgaacgccgg tgtgatagtc atcggatcgc
     6901 gacctgcgcg cgtggctgaa tcgcgcacca ccttgagctt ggctgcatag tcttctggtg
     6961 aatacgcgcc tgcgggccac cagccgtcgc agtagcggcc gatgatatcc agcatgcgcg
     7021 gtccgctgcc accggcccag attctcggaa cacggccttc atacggctcg gtgtcgagcc
     7081 gtgcatgacg cagcttgtag aactgccctg agaaatccac cgggccttcg cttttccaca
     7141 gcagttggat gacctgcagc gcttcctcga agcgggctac cggcttggca aaatcgaatc
     7201 cgtagggcac ggtattttcg gtctcgccgc tgcctacgcc caggatgaag cggccccggg
     7261 atagatgatc gatggtcagc gccgactgcg ccaacaggga cggatgacgt cgcaccgtgt
     7321 ccacgacgct ggttgcaatc gtcacattct tggtcagggc ggcgacggcc gcggccacgg
     7381 cgagtccatc cagatgacga tgcggcgagg cagaggtagt cgcgagatcg gtaaattccg
     7441 cagtccagat cgcatccggc cagaagctca ccatgtgatc gggaagccag accgagtggt
     7501 aacggccgct atccagggca tcaagcttgg ataggtccag tggaagcgtc gtacgaacaa
     7561 aagccgccgg ttgaacgttc attgctcgct ccattcgtca gcgaatctcg gccccgaaga
     7621 ttattaccga gccatccttc gtcaagccca agccggtcca tctgcatacc gttctcatgt
     7681 gcggatagaa tccgccagct cgagtgccgc gcgcgcaacc cgtatccgat catccagcgt
     7741 cttcatcagg gtatcggcgc gtgcctgagc aagttcgaat gcggcgggcc cgtcgcgctc
     7801 atcgatcagc attccgtcga tgagctcgcc gtagtgccgg acgagcgcgg tagcgctgac
     7861 ctctatgccg agttcgcgca tcaatttggc ggtcggtccc ttcaccgcgg ttccgccgac
     7921 gagcggcgac accgcaatca cgggcacgtc ggtctgcatg agcgcctgtc ggatggaggg
     7981 caccgccaga atgggatcga tgctcagaaa aggattggat ggtgcgatca gtatggcacg
     8041 cgtgtgcgcg tctcggagcg cctccagcac gccaggcgcg gcttgcgcgg acgccgcgcc
     8101 gtggaagcgg attgccttca ccacgggctc acactttcga gcgacgaaat actgctggaa
     8161 ttcaagttca ccctcctcag cctgtactcg cgtcgcaacg ggatcgttac tcattggcag
     8221 gattttcgcc ttgatccgcc aggctgtcgc aaagtcggcg atgatcgccg acagtgcctc
     8281 tcccgagcgc agccgctgcg tacgaagcac atgcaaggcg aggtcgccgt ccccgagcgc
     8341 gaaccaatgc gggcccccga gtgaccgcat cgcctccatg aagctccagc tctcaccctc
     8401 gcgaccccag ccctgtgcga cgcttg
//
LOCUS       DATMRM010000459         2720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1513592, whole genome shotgun sequence.
ACCESSION   DATMRM010000459 DATMRM010000000
VERSION     DATMRM010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513592"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1263)
                     /locus_tag="VNZ06_13935"
     CDS             complement(64..1263)
                     /locus_tag="VNZ06_13935"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWW21895.1"
                     /translation="MNDLRIVDVSEIIAQTASALSGASGCTVKIDEMQRVSDERRRNF
                     IGRARAVDAEGRVRSVLVKATRSVSYDPAAEDALENSGLVREWVAAAYIAAQAPGRGH
                     GAALLAGDVSRGLLVFEDLGIDLPSLVDPLLKCTADEAERALTLYAGALGHLHADTAN
                     CLASHHATFQSIFGADRPRRPSGWRIEKQADLIVDRLGGAPPASELELLSARHRDPGP
                     WQCLVHGDPCPDNALIVDGSVRLIDYEHARPSHALLDGIYWQIGFPTCWCAGRVPADV
                     ARRIADVYRAEITKAMPLARDDFAYRAELAYVAAIWLFTCLSWHLEAALKDDSKWGIW
                     SIRGRLLWYLQAVIEMTTSADVLPGLNEAAHRWLSELRRRWPDALPLGLYPAFAASCD
                     SSAMDFR"
     gene            complement(1322..2113)
                     /locus_tag="VNZ06_13940"
     CDS             complement(1322..2113)
                     /locus_tag="VNZ06_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21896.1"
                     /translation="MIARADEQLAGVRERIASVDEEIARVTERLSKMERDDAGNPSAR
                     PEDDARNPVARPGPQPSREKSAVPGSINLLPIGVLLAACLVVAALVPQLYRDAVTLIA
                     ARWTPQRVSTSFSQPANPPLSAQPDPPFKLIMAEEAAPAQAAPLARTGPQDAASAAAG
                     ISELMQLQQATARDVANLKQEIEQLKVKQDQIATDNSKAIEQLKAMREDVARLFAKVS
                     EQSALPKTSPPPARPTLSLQARMRPQAPPPRRRYSPRWQYYDDEW"
BASE COUNT          526 a          847 c          841 g          506 t
ORIGIN      
        1 gacgcaaagc ccgtgtatca tttcgcttgg acaaacgcga caccatgacc atccgagcgc
       61 ggcctagcgg aaatccatcg ccgacgagtc gcagctcgcc gcgaatgccg gatagagtcc
      121 gagcggcaag gcatcgggcc agcgacgccg cagctcggac agccagcgat gcgctgcctc
      181 attgaggcca ggcagcacgt ccgcggatgt ggtcatttcg atcacggcct ggagatacca
      241 gaggagccga cctcttattg accagatgcc ccatttcgaa tcgtccttca gcgccgcttc
      301 gaggtgccag gaaaggcacg tgaagagcca gatcgccgcc acgtaggcga gttcggcacg
      361 ataggcgaaa tcatcgcgcg caagcggcat ggcctttgtg atctcggccc gatagacgtc
      421 ggcgatacgt ctcgcgacat cggccggaac acgacccgca caccagcagg tcggaaatcc
      481 gatctgccag tagatgccat caagcagcgc atgcgatgga cgcgcatgct cgtagtcgat
      541 caggcgcacg ctgccgtcca cgatcagggc gttgtccggg cagggatcgc cgtgaacgag
      601 acattgccat gggccgggat cacggtgtct cgcggatagc agctccaatt cgctggccgg
      661 tggcgcgccg cccagcctgt ccacgatcaa atcggcctgc ttctcaatgc gccatccaga
      721 tggacggcgc ggacggtcgg cgccgaagat ggattggaac gtcgcatgat gcgacgccag
      781 gcaattggct gtatcggcat ggaggtgccc caatgcacct gcgtaaagcg tgagtgcgcg
      841 ctctgcctca tcggcagtgc atttgagaag cggatcgacg agcgacggca ggtcaatgcc
      901 gagatcctcg aagaccagaa ggccgcgcga aacatcgccg gccagaagcg ctgcgccgtg
      961 gccacggcct ggagcttgcg cggcaatata tgcagcggct acccactccc tcaccaggcc
     1021 tgagttttcc agcgcatcct cggcggcggg atcatagctc accgatcgcg tcgccttcac
     1081 gagaacagag cgaacacgcc cctcggcgtc aaccgcgcga gcgcggccaa taaagtttcg
     1141 cctccgctca tcactgacac gctgcatctc gtcgatcttt accgtgcagc cggaggcgcc
     1201 ggagagcgcc gaggcggtct gggcaatgat ttctgaaacg tcaacaattc gcagatcgtt
     1261 catggcgagc catcgattgc gttcgagcgc cagactttca acgattgcta cgttactgcg
     1321 attaccattc gtcgtcgtag tactgccacc gcggcgagta ccgccggcgc ggcggtggcg
     1381 cctgcggccg cattctggct tgcagcgata gggtcggtct tgccggaggc ggtgacgtct
     1441 tgggcaacgc gctttgctcg gaaaccttgg caaacaggcg cgccacgtct tcccgcatcg
     1501 ccttgagctg ctcaatggcc tttgaattgt cggtggcgat ctggtcctgc ttcaccttca
     1561 gctgttcgat ctcttgcttc agattcgcga catcgcgcgc cgtcgcctgc tgcaactgca
     1621 tcagctcgga gatcccggca gccgctgacg cggcgtcttg cggcccggtc cgagcgagcg
     1681 gtgctgcttg cgcgggcgcg gcctcctctg ccatgatcag cttaaagggt ggatcgggct
     1741 gcgcggacaa cggcgggttt gctggctgcg agaatgacgt tgaaacgcgt tgcggcgtcc
     1801 aacgggctgc aatcagcgtg acggcgtcac gatacaactg cggcacgagg gctgcgacga
     1861 caaggcacgc cgccaacagc acgccgatag ggagcaggtt gatcgagcca ggcaccgctg
     1921 acttttctcg cgatggctgc gggccgggtc tggccacggg attgcgggca tcatcctcgg
     1981 gtctggccga agggttgcct gcatcatccc gctccatttt tgaaagccgt tcagtcacgc
     2041 gtgcgatctc ttcatctaca ctcgcgatcc gttcacggac acccgcgagt tgttcatcgg
     2101 cgcgcgcgat caagacatcc tttgtattcg gtgtggagtt cattggcgtt tccctctccg
     2161 tccaatgtca tccgcggatg gggtcaaaca tccagcaggt cgaccgccag ccaaagacct
     2221 caatgcaatc ttgtaagact cggacgacag aatgtacagg accacgtcac ctgacgcgcc
     2281 aagtccgacc gtgcggccag cgcgcgcggg ggaatggagg cttcaatgaa gtgcctgcgc
     2341 attgactcga cagcggatgg cgtgtcgcat ttcgatcaag tcgaaatttc cgacaagctc
     2401 ccggcaggtt cacctccaga ccacagcgtt cgaattgtcg gcaaattatg cggcgacacg
     2461 ccatccgttt cacgcgcatt ccgtcgggcg cgcgacattc agccaacctt gctcgcccgc
     2521 accgacggag gtgatcgagt agggctcgct ttgctgcggt gcatgagtcc gaaaaggcgg
     2581 cgatctgcgc cacggtcaag gccacaccgg agtttgccga gcgcgccgag atcattgaaa
     2641 gtgtgccggg tctggccgaa acggcctctg cgaccctcat tgcagggatg cctgaactcg
     2701 gacaggtgag cgacgtggtt
//
LOCUS       DATMRM010000460         5097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1203336, whole genome shotgun sequence.
ACCESSION   DATMRM010000460 DATMRM010000000
VERSION     DATMRM010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203336"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VNZ06_13945"
     CDS             complement(<1..461)
                     /locus_tag="VNZ06_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HWW21897.1"
                     /translation="MIRGQQIPEEVGRYSLLAIALLYLVALVVLPLLIVFFEAFAKGI
                     GPYIDALLDPYALAAMRLTLLTSAVAVPLNVIFGVCAAWAIAKFEFRGKNALITLIDV
                     PFAVSPVISGLIYVLMFGAQGLLGPYLMEHDIHIIFAVPGIVLATTFVPFP"
     gene            complement(458..1342)
                     /gene="cysT"
                     /locus_tag="VNZ06_13950"
     CDS             complement(458..1342)
                     /gene="cysT"
                     /locus_tag="VNZ06_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HWW21898.1"
                     /translation="MTDASIAMTLQPDEDPAPRARSSFRILPGFGLTMGFTITYVSLL
                     VLIPLSVTFVRSAELGPEKFWQVVTAPRVLASLRLSFGAALLAAAVNAVMGFIIAWAL
                     TRQRFPGRRLFDAIIDLPFALPTAVAGIALTALYAPNGWIGQLLVPFGVRVAYTPLGV
                     VVALAFVSLPFVVRTLQPVLGDLDREVERAAETLGASRLQTFLWVVLPSVWPTLLTGA
                     ALGFSRAVGEYGSVIFIAGNIPKVSEIAPLMIMTKLEQYQYAEATAIAVVMLTASFGL
                     MFGINALQLWAQSRRQTR"
     gene            complement(1347..2384)
                     /locus_tag="VNZ06_13955"
     CDS             complement(1347..2384)
                     /locus_tag="VNZ06_13955"
                     /inference="COORDINATES: protein motif:HMM:NF008022.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWW21899.1"
                     /translation="MPAVVTRRRALSLLAGASGALAAGCSENSQPSGKEPLTLLNVSY
                     DPTRELYEAINKAFIAQWLAQSGEQLTIRQSHGGSGKQARAIIDGLQADVATLALAYD
                     LIELHKQARLIPEDWAARLPNNSAPYTSTIVFLVRRGNPKNLRDWDDLIRPGVQVITP
                     NPKSSGGARWNYLAAWGWAAKQPGGDDAHAGEYMGKLFANVPVLDSGARGATVTFVDR
                     GIGDVLLAWENEALLAKQNLGADKLEIVIPSLSILAEPPVTVVDKVVDRRGTRKVAEA
                     YLQYLYTNEGQRIIAQNFYRPRAAEVMQQFSKQFPALKLVTIDDTFGGWEQAQHKHFD
                     DGGSFDQIFGH"
     gene            complement(2384..4246)
                     /locus_tag="VNZ06_13960"
     CDS             complement(2384..4246)
                     /locus_tag="VNZ06_13960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWW21900.1"
                     /translation="MSINFEQITKRYHSAPVVNDVSLQIEAGEFFVLLGPSGSGKSTL
                     LRIAAGLTDVDHGRISFHGRDVTHVRARDRGVGLVFQHYALFRHMTVGENVEFALRVR
                     GVRRRDRARRRDELLQLVALEGFAGRLPGQLSGGQQQRVAVARALAHEPQVLLLDEPF
                     GALDAKIRVELRDTIRQVQRRLGMTTILVTHDQEEAFALADRIGIMHNGRLLETGRAE
                     TLYRRPASRFVATFLGAANLFLGERSHQGLRIGNHFLASADSLKAADAGNEVVTIVRP
                     EDVEIVEDTERLRSAHLSDGHVLSVDFGGVFERLRIQLTRESGVVSAIGREHLPDASE
                     AAIHTPDVVIDAMRTNTEQGVFPLQVGKRVTLGIRRFHVLPTPISSFRILSAAAAAVN
                     LLMRSPLLRQLVQSTQAPVLDAQDTRERDTAQTGVGVIAGGAGAINEIVAAAAQGRHR
                     VLVIPPDSTLPRRMLIYVQGEAARSATLGLVASLMRHLHAEATFVSVQSPMAPRSEVT
                     NSFRRLLDARAELQETHGLDIRTDVQIGDLNSWVSHLAASTESSLIVLGMDGSPRELE
                     QRLTEDFRPLLNEHARSPVLLSFSGLPWIADGRVGGIHSLFDSQADDAIQGNAN"
     gene            4405..>5097
                     /locus_tag="VNZ06_13965"
     CDS             4405..>5097
                     /locus_tag="VNZ06_13965"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HWW21901.1"
                     /translation="MKVTIFGSGYVGLVTGACLADTGNHVVCVDIDASKVERLNRGEV
                     PIHEPGLDVLIKRNREAGRMEFTTDSARGIEHGLFQLIAVGTPPDEDGSADLRHVLSV
                     ARAIGTHLTRYAIVITKSTVPVGTADRVRMEVHSALAARGAAVEFDVVSNPEFLKEGA
                     ALDDFMKPDRVVVGTDNPRTTELMRALYEPYTRNHDRLVVMDIRSAELTKYAANAMLA
                     TKISFMNELANLA"
BASE COUNT          991 a         1605 c         1618 g          883 t
ORIGIN      
        1 aacgggaagg gcacaaacgt ggtggccagc acgatgccgg gaacggcaaa aatgatgtga
       61 atgtcgtgct ccatcagata gggcccgagc aatccctgag cgccgaacat cagcacatag
      121 atgaggccgg agatgaccgg cgagaccgcg aacggcacat cgatcagtgt gatgagcgcg
      181 ttcttgccgc ggaactcgaa cttggcaatg gcccacgccg cgcacacacc gaagatgacg
      241 ttgaggggca cggcgaccgc ggaggtcagc agtgtcagcc gcatggcggc cagcgcgtag
      301 ggatccagca gcgcatcgat gtaagggccg atccctttgg cgaaggcctc gaagaacacg
      361 atcagcaacg gcagcaccac cagcgcgacc agatagagca gggcgatcgc gagcaacgaa
      421 tagcgcccga cttcctccgg aatctgctga ccgcgaatca tcgcgtctgg cggcgcgact
      481 gcgcccacag ttgcagggcg ttgataccga acatcagtcc gaaggaggcg gtcagcatca
      541 cgaccgcgat cgcggtggct tcggcgtact gatactgctc gagcttcgtc atgatcatca
      601 gcggggcgat ttccgatacc ttcgggatgt tgccggcgat gaagatgacc gaaccgtatt
      661 caccgaccgc acgtgagaag cccagtgccg cgccggtgag cagtgttggc cacaccgaag
      721 gcagtaccac ccacaggaag gtctgcagcc gcgaggcgcc gagtgtctcg gcggcacgtt
      781 caacctcgcg gtccaggtcg cccagcaccg gctgaagcgt gcgaaccacg aacggcagac
      841 tgacgaaagc gagcgccacg accactccca aaggggtata ggccacccgt acgccaaacg
      901 gcaccagcag ctgaccgatc caaccgttcg gcgcatagag cgcggtgaga gcaatgccgg
      961 ccacagcggt aggcaatgcg aacggcagat cgatgatggc gtcgaacaac cgccggcccg
     1021 ggaaacgctg ccgcgtcaat gcccaggcaa tgatgaatcc catcaccgca ttgacggccg
     1081 ctgccagcag cgcggcgccg aaactcaggc gcagcgaggc cagaacacgc ggtgcggtga
     1141 ccacctgcca gaatttttcc gggccgagct ccgccgatcg cacaaacgtt accgatagtg
     1201 ggatcagcac cagcagcgac acatacgtga tcgtgaagcc catggtcagt ccgaagccag
     1261 gcaggatgcg gaagctgctg cgggcgcgcg gtgccggatc ctcgtcgggc tgaagcgtca
     1321 ttgcgatgct ggcgtcggtc atcgattcag tgtccgaaga tctggtcgaa gctgccgccg
     1381 tcatcgaaat gcttgtgctg ggcctgctcc cagccgccga aggtatcgtc gatcgtcacc
     1441 agcttcagcg ccgggaactg cttcgagaac tgttgcataa cctccgcggc gcgcggccgg
     1501 tagaagttct gcgcgatgat ccgttgcccc tcgttggtat acaggtactg caggtacgcc
     1561 tctgccactt tgcgcgtacc gcgtcggtcg acgaccttgt ccacgactgt caccggaggt
     1621 tcggccagga tgctcagtga ggggatcacg atctccagtt tgtctgcacc cagattttgc
     1681 ttggcgagca gcgcctcgtt ctcccacgcg agcagtacgt cgccgatacc gcgatcgacg
     1741 aatgtgacgg tcgcaccgcg cgcccccgag tcgaggaccg gcacattggc gaacagcttg
     1801 cccatgtact caccggcatg ggcgtcatcg ccaccaggct gtttggccgc ccagccccag
     1861 gccgccagat agttccaacg cgcaccgccc gagctcttgg ggttcggtgt gatcacctgt
     1921 acacccgggc ggatcagatc gtcccagtcg cgcaggttct tgggattgcc ccgacgcacc
     1981 aggaagacga tggttgaggt atagggtgcg ctgttgtttg gcagtcgcgc cgcccagtct
     2041 tccggaatca gccgcgcctg cttgtgtagc tcgatgaggt cgtaggccag cgccagtgtg
     2101 gcgacgtccg cctgcaagcc gtcgatgatg gcgcgggcct gcttgcccga gccaccgtga
     2161 ctctgtcgga tggtgagctg ctcgccgctc tgcgcgagcc actgggcaat gaaggccttg
     2221 ttgatcgcct catacaactc gcgggttggg tcatacgaga cgttcagcag ggtcagcggc
     2281 tccttgcctg aaggctgaga attctccgag cagccagctg ccagggcgcc tgacgcgcca
     2341 gccagcaggc tcagcgcgcg ccgtcgggtc acaacagctg gcatcagttg gcgttccctt
     2401 gaatggcgtc gtcggcctgt gagtcaaaca aagagtgaat gccgccgacg cggccgtcgg
     2461 caatccacgg caatccagag aaggagagca ataccggact gcgcgcgtgc tcattcagca
     2521 gcgggcggaa atcctcagtg aggcgctgtt ccagctcgcg cggcgagccg tccattccca
     2581 gcacgatcag cgaggactcg gtcgatgcgg ccaggtgcga gacccagctg ttgagatcac
     2641 cgatctgcac gtcggtccgg atatccagac catgcgtctc ctgtagttcc gcacgcgcat
     2701 ccagcaggcg tcggaacgag ttggtgactt ccgaacgcgg tgccatgggg ctttgcacgc
     2761 tgacgaaagt cgcctcggcg tgcaggtgcc gcatgaggct cgcgaccagt ccgagggtgg
     2821 cggaacgcgc cgcttcgccc tgcacgtaga tcagcatgcg acgcggcagc gtggagtccg
     2881 ggggtatgac gagcacacgg tgtcggccct gtgccgccgc ggcaacgatt tcgttgatcg
     2941 cgccggcgcc cccggcgatg acgccgacac cggtctgcgc ggtatcgcgc tcacgcgtgt
     3001 cctgcgcatc cagcaccgga gcctgcgtgc tttgcaccag ctggcgcagc agcggcgagc
     3061 gcatcaacag gttcacggcg gcagcagcgg cgctgagaat ccggaaactc gagatcggcg
     3121 tgggcagcac gtggaatcgc cgaataccga gcgtcacacg tttgcctacc tgcagcggaa
     3181 acacgccctg ttcggtgttg gtgcgcatgg cgtcgatcac gacgtcaggg gtgtggatcg
     3241 ccgcttcgct tgcatcgggc agatgctcac gaccaattgc cgaaaccacg ccgctttcgc
     3301 gagtgagctg aatgcgcagc cgttcaaaca cgccaccgaa gtccaccgac aggacatggc
     3361 cgtcgctcaa atgcgcgctg cgcagccgct cggtgtcttc cacgatctcc acgtcctccg
     3421 gccggacgat ggtcacgacc tcgttgcccg cgtcggccgc cttgaggctg tcggcgctcg
     3481 caagaaaatg gttgccgatc cgcagcccct gatgactgcg ctcgccgagg aacagattgg
     3541 cggcgcccag gaaggtggcg acgaagcgac tggcgggtcg ccggtagagt gtttccgccc
     3601 ggccggtctc gagcagccgc ccgttgtgca tgatgccgat gcgatcggcc agcgcgaacg
     3661 cttcctcttg atcatgcgtg accagaatcg tcgtcatgcc gaggcgtcgc tgtacctggc
     3721 ggatcgtgtc gcgcaactcg acgcggatct tggcatcgag cgcgccgaaa ggctcatcga
     3781 gcaatagcac ctggggctca tgcgccagcg cgcgcgcgac cgccacccgt tgctgctgtc
     3841 cgcccgacag ctgtcccggc aggcgcccgg caaaaccctc gagcgccacg agttgcagca
     3901 gttcgtcgcg acggcgtgcc cgatcacgac gccgcacccc gcgcacccgc aacgcgaatt
     3961 cgacgttctc cccgacggtc atatgacgga acagggcata gtgctggaag accagtccga
     4021 cgccgcgatc tcgggcacga acgtgcgtta cgtctcgtcc atgaaaggag attcgcccgt
     4081 gatcgacgtc ggtcaatcct gcggcgatgc gcagcagggt actcttgccg ctaccgctgg
     4141 gtccgaggag tacaaagaac tctccggcct caatctgtag cgagacgtca ttgacgaccg
     4201 gggcgctgtg atagcgcttc gtaatctgct cgaaattgat cgacatgcat gcttaccgcc
     4261 cgggtatcgt atgaatttcc gggtgccggg tcagttccaa gctggtaaca atgcagttat
     4321 gaaaaatcga tcacggttta cgctttgcct tgacccagcg cttgcgctat cgtgcgtttt
     4381 ccttccattt caaagattta acgaatgaaa gtcaccatct tcggctcagg ctacgtgggg
     4441 ctggtcaccg gcgcttgctt ggccgatacc ggcaatcacg tggtctgcgt ggacatcgat
     4501 gccagcaagg tcgagaggct gaaccgaggc gaggtgccga ttcatgagcc ggggctcgat
     4561 gtcctgatca agcgcaatcg cgaagccggg cgtatggagt tcaccaccga cagcgcccgc
     4621 ggtatcgagc acgggttgtt ccagctgatc gcggtgggaa cacccccgga cgaagacggt
     4681 tcggccgacc tgaggcatgt gctgagtgtg gcgcgcgcca tcggtacgca cttgacgcgc
     4741 tatgcgatcg tgatcaccaa gtccactgtg ccggtcggta ccgctgaccg ggtccggatg
     4801 gaagtgcact cggctttggc cgcgcggggc gcagcggttg aattcgacgt ggtctccaac
     4861 cctgagttcc tgaaggaggg cgcggcgctg gatgacttca tgaagccgga tcgtgtggtc
     4921 gtcggcaccg acaacccgcg caccaccgag ttgatgcgag cactgtacga accctacacg
     4981 cgcaaccacg atcgcctggt ggtcatggac attcgctcgg cagaactcac caagtacgcc
     5041 gccaacgcca tgctcgcgac caagatcagc ttcatgaatg agctggccaa tctggcg
//
LOCUS       DATMRM010000461         4861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1516521, whole genome shotgun sequence.
ACCESSION   DATMRM010000461 DATMRM010000000
VERSION     DATMRM010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516521"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..520)
                     /locus_tag="VNZ06_13970"
     CDS             complement(197..520)
                     /locus_tag="VNZ06_13970"
                     /inference="COORDINATES: protein motif:HMM:NF027545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4880 domain-containing protein"
                     /protein_id="HWW21902.1"
                     /translation="MNDRDDRARDVTQEAAEWYVANRAGLDATQRVRFTHWLKFSPAN
                     VREYLAIAAVVRDLRGGPWTVLLSAPSAACAAVSGPSPESKRGVAAGTLGASLQDGSS
                     YHGEA"
     gene            complement(1068..1610)
                     /locus_tag="VNZ06_13975"
     CDS             complement(1068..1610)
                     /locus_tag="VNZ06_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21903.1"
                     /translation="MKRKFLTAVTAGTWLAFSALAGAQSAGAPGAAAPGKDQHVISSQ
                     DLDLLRHDIRSQKKQLIAQNLTLTDSDATKFWPIYDKFTAELVKINDKKYAALQEYAD
                     HWGSLTDDQAVALAKQVQEVDVQAAQLRAKYLPIVTQAIGGKKAATFTQLERRISLLI
                     DLQLASKIPLVQSQNQGQSQ"
     gene            2082..4313
                     /locus_tag="VNZ06_13980"
     CDS             2082..4313
                     /locus_tag="VNZ06_13980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWW21904.1"
                     /translation="MKLAALISECTRRLAINPGRLPQVELTSILQPLLERAQLDVFCL
                     VRMPAGASPEIVYHVEPHGSPLPAQAARANQWLPWLAEKFAKDPKQLLIRSVAELSPS
                     VRAAWRSLSGGRVRSLLLVPALMSQEVRYVGVFGCSDDHAAGTDGDARHLKKLAEGVA
                     LVIGRCDSHEVTDQARKFEQLMSRLLRGFGAAHWTQIDAQIGRALEDIRNFLDVDQCA
                     LLTIGPGDGEATIANLASRDIDFPLGVPVNYSVAVPWLFGRLVNRRETIAFSRPEDLP
                     SDAAIDRRYLESVRTHAAVYVPYTMDGLARFIFSVVVNDRARSWSPGVIEQIEAVGAA
                     FLSPIRRKTTGAAGAQAQEQLVSAQQFSQATLDALHKYVAVIDVTGQLVRASDLWGSI
                     GGDTPCVLDAVKGGGNLLAALETLQGPLRERALRLSEGVRSVLSGDVPCFDTELNLQP
                     SQAPRWLHVRVQQFVVYGSVFAAVSYEDVTQRHQREQELQLLRDHQWHSDRVAQTGVL
                     IASLAHELSQPLAAVLINAQTGLRLLADDKLIPQLNREILSDIVADCKRAGAVIESLR
                     IMLRRRKTEQKPEDLAEIVSRIVRLLHSEAVDQRVEIAERYQDGCVVTADRAQIQQVA
                     LNLIVNAIEAMADVPAPNRHLRVSVARWRDDKVLLSVRDSGVGITEEQMDKVFEAFWT
                     TKAHGTGMGLPICQAIVETHGGTIWIKRQKVGSTFYVALPLAHVQPLTKQLQSRGRRG
                     KAA"
     gene            4310..>4861
                     /locus_tag="VNZ06_13985"
     CDS             4310..>4861
                     /locus_tag="VNZ06_13985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW21905.1"
                     /translation="MIGTATDDTTIFLIDDDESLRRSLSRQLTHAGWKVEAFASALDF
                     LERASYEGTGCVVLDVRLPEMMGPELHVEMVNRGMTLPVVFLTGHGDVPTSVNAMKRG
                     AVDFLTKPVDGDLLAATIRAAIDRHAVQRRSDIQQQAFEERLRLLSPREREVLDFVVA
                     GCLNKQIADKMGITLKTVKVHRAR"
BASE COUNT          919 a         1494 c         1546 g          902 t
ORIGIN      
        1 tctatttcct cgataacgaa gtaatcatcg aaccatcgcc cggcgaccgg cgcgcttggc
       61 ccgggcagca gcggcgcgtc tttcccatga attcgatgct gatactcggg gtaagcgtaa
      121 ggggcgacga acgcgaaaac cgccgtggcc actgcgagga tggccagtga caatgagacg
      181 cgacgccgca cggagttcat gcctcgccat gataagaaga tccgtcctgc aatgaagcgc
      241 cgagcgtacc ggcagccaca ccgcgcttcg actcggggct cggccccgag actgccgcgc
      301 acgcggctga cggcgctgag agcaacaccg tccacggccc tcctcgaagg tcacggacga
      361 cagccgcgat ggccaggtat tcccttacat tggccggcga gaacttaagc cagtgggtga
      421 acctcacccg ctgagttgcg tccagccctg cgcggttcgc gacgtaccac tcggcggctt
      481 cctgcgtcac gtcgcgcgcc cgatcatccc ggtcgttcat gccacctcaa tccgtgctgc
      541 gggccggcgg cccaataacg gccgtcaccg ggggaatcat cgcctgaagg cgggcacctc
      601 cgcggaagct cagacgagcg aaggagctca agtcacgatt gcgcctggcg acgggctgct
      661 ccgggcagcg ctattgcgcg caaggactta agcgctctcc aggcctccgg ggtcaacagc
      721 aaagagccgc attcgtgaag ttcgggtacc agaaaacgca gcgccttggc gcagacacca
      781 acagcgccga gccctcgtga ggcgacgaag gcgcgagcgg tagctgaaac tctgcctggg
      841 tcctcagggc aaaaactgcc tgcagcagtc cagggtcgat tgaggatctg tatctcggga
      901 actggcggcc gactgccgtg cgcctaggaa tcctgcgttg tgcttattct gctggcaacg
      961 cggcttgcgt ggcgctcccg aaaaaggagc gccacgcatc atcagccgac atgcgctcga
     1021 ctcgccagct ccaacatccg gacagccgca gggtctaagt tagtgcgcta ctggctctgg
     1081 ccctggttct gactctggac cagggggatc ttggacgcga gctgcaggtc gatcagcagc
     1141 gaaatgcgac gctcgagttg cgtaaacgtc gcggccttct taccaccgat ggcctgcgtc
     1201 acgataggca gatacttcgc ccgcagttgt gcggcctgga cgtctacttc ctggacctgc
     1261 ttagcgagag cgacggcctg atcgtcggta agactgcccc agtgatccgc atattcctga
     1321 agcgctgcgt acttcttatc gttgatcttc accaactcgg ccgtgaactt gtcgtagatc
     1381 ggccagaatt tcgtggcgtc actgtcggtt agagtcaggt tctgcgcgat cagctgcttt
     1441 ttttgcgagc gaatatcgtg acgcagcagg tccagatcct gcgagctgat cacgtgctga
     1501 tccttaccgg gtgcggcggc gcccggagcg ccggccgact gtgccccggc caatgccgag
     1561 aaagcgagcc acgtgcccgc tgtcacagcc gttaaaaact ttctcttcac aatcatctcc
     1621 ttcgttgtat tgcaacaaat actgcgagac cgcttgtatg caacacatac tgccagaccg
     1681 cctgaatggc cttcccgccc gcagaatcga gttgaaaccg gcaagacccc tgatttgcga
     1741 ttcagctttc gagctctgat gttcggttga ccgcttaaat ccgaacgtgg actgagccca
     1801 agcagcgtga ggtcgtacgg gcgaccctac ggagttcgac ggcgaccaca attggacctt
     1861 gggcccattc gcgcatgatc aaagccggaa attccgctaa gctgtagacc gcgtccaagg
     1921 aaatctccat ctggtcatgg tcgcccggat gaagatgctc gcagggtcga aagaagaaaa
     1981 tgccaccgga tccgtataaa tctggcagcg tgctgtcgga accctttgag aggtttggag
     2041 cactccggcc ccgaccgcgg gacgtcccgc gttctcgatc ggtgaagctc gcagcgctta
     2101 tttccgagtg cacgcgccgg ctggccataa atcccggacg gctgccgcag gtcgagctga
     2161 cgtcgatact gcagccgctg ctcgaacgtg cgcagctcga cgtgttttgc cttgtcagga
     2221 tgccggcggg cgcgagcccc gagatcgttt atcacgtcga gccacacgga tcaccgttac
     2281 ctgcgcaggc cgcgcgcgcc aaccagtggt tgccgtggct ggcggagaag ttcgccaagg
     2341 acccgaagca gctgctgatc cggagcgtcg ccgagctgtc gccgtccgtg cgggccgcct
     2401 ggcgtagctt gagtggcggg cgtgtgcggt cattgctgtt ggtgcccgcg ctgatgagtc
     2461 aggaagtgcg ctatgtgggc gtgttcggtt gcagcgacga ccatgccgcc ggtaccgacg
     2521 gcgacgctcg acacctgaaa aagcttgccg aaggcgttgc cctcgtcatc ggccgatgtg
     2581 attcgcacga ggtaacggac caggcgcgca aattcgagca gctgatgagc cggctactgc
     2641 gtgggtttgg tgctgcgcac tggacgcaga tcgacgcgca gatcggtcgc gccctggaag
     2701 acatccgcaa cttcctggat gtggaccaat gcgcgctgct gacgattggg ccaggcgacg
     2761 gtgaggcgac catcgccaac ctggctagtc gtgacatcga ctttccgctg ggcgtaccgg
     2821 tcaactactc cgtcgccgtg ccgtggctat ttggcaggct ggtaaatcgg cgcgaaacga
     2881 tcgcgttcag ccggccagag gatctgccgt cggacgccgc gatcgaccgg cgttaccttg
     2941 agagtgtgcg cacccatgcg gcggtgtacg tgccttacac gatggatggt ctggcgcgct
     3001 tcatcttctc ggtggtcgtg aacgaccgtg cgagatcctg gtcgccgggt gtgatcgagc
     3061 agatcgaggc cgtcggcgcg gcctttctga gtccgattcg ccgcaagacc accggtgctg
     3121 cgggagcgca ggcacaagag cagctggtca gtgcacagca gttctcccaa gcgacgctcg
     3181 atgccctgca caaatacgta gccgtcatcg atgtcaccgg tcagctcgtc cgtgccagcg
     3241 acctgtgggg cagcattggc ggcgacaccc cgtgtgtgct cgacgcggtg aagggcggcg
     3301 gtaacctctt agccgcactc gagaccctac agggaccgct gcgcgagcgg gcactcaggc
     3361 tttcagaggg cgtgcgcagc gtgctcagcg gtgatgttcc gtgcttcgac acggagctga
     3421 atctgcagcc ctcgcaggca ccgcgctggc tgcacgttcg cgtgcaacag ttcgtagttt
     3481 acgggtcggt gtttgcagcc gtgtcctacg aggacgtgac gcaacggcac cagagggaac
     3541 aggagttgca gctgctgcgc gatcaccagt ggcattccga ccgggtcgcc cagaccgggg
     3601 tcctgatcgc atcactggcg cacgaactca gtcaaccgct ggcggccgtc ctgatcaatg
     3661 cgcagacggg cttgcggctg ttggccgacg acaagctcat cccgcagctc aatcgcgaaa
     3721 ttctcagcga catcgtggcc gattgtaagc gcgccggagc ggtcatcgag tcgctgcgca
     3781 tcatgctgcg gcgccggaag accgagcaaa aacccgaaga tctggcggaa attgtcagtc
     3841 gcatcgtccg cttgctgcac agcgaggccg tggatcagcg cgtggagatc gccgaacgct
     3901 accaggacgg ctgcgtcgtc accgccgatc gggcgcagat ccagcaggtg gcgctgaacc
     3961 tcatcgtgaa tgcgatcgag gcgatggcgg acgtgcccgc gccgaatcgg cacctgcggg
     4021 tcagcgtcgc gcgctggagg gacgataagg tgcttctgtc ggtgcgtgac agcggcgtcg
     4081 gcattaccga ggagcagatg gacaaagtct tcgaggcgtt ctggaccacg aaggcccacg
     4141 gcacggggat gggacttccg atctgccagg cgattgtcga aacgcacggc ggcacgatct
     4201 ggatcaagcg ccagaaggtc ggttcgacct tttacgtcgc gctgccgttg gcgcatgtac
     4261 aacctttgac taagcaattg cagtcgcgcg gccggagggg caaggcggca tgattgggac
     4321 ggcgacggac gacaccacca tctttctgat tgacgacgac gagagcctgc gtcgcagtct
     4381 ttcccgccag ctgacgcacg cgggctggaa ggtcgaggca ttcgcgagcg cgctggactt
     4441 tcttgaacgc gcctcgtacg agggcaccgg gtgcgtcgtg ttggacgtgc gcttgccgga
     4501 aatgatggga ccggagttgc acgtcgagat ggtgaatcgc ggcatgactc tgcccgtcgt
     4561 ctttctcaca ggtcacggcg acgtgccaac ttccgtcaac gccatgaagc gcggcgctgt
     4621 cgactttctc accaagcctg tcgatggcga tttactcgcc gcgaccatcc gcgcggcgat
     4681 tgaccgtcac gcggtccagc gccgctcgga tatccaacag caggccttcg aggaacgtct
     4741 gcgactgctc tcgccgcgcg agcgcgaggt gctggatttc gtggttgccg gctgccttaa
     4801 caagcagata gcggacaaaa tgggaatcac cctgaaaacc gtcaaggtgc accgcgcccg
     4861 c
//
LOCUS       DATMRM010000462         4052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2747697, whole genome shotgun sequence.
ACCESSION   DATMRM010000462 DATMRM010000000
VERSION     DATMRM010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2747697"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1681
                     /locus_tag="VNZ06_13990"
     CDS             <1..1681
                     /locus_tag="VNZ06_13990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW21906.1"
                     /translation="LMQILKNLLSNAFKFTDHGGVRLHVGVAAGGWSADHPVLSQAQP
                     VIEFSVSDTGIGIPPEKQRIIFEAFQQADAGTARKYGGTGLGLAISRELAHLLGGEIR
                     LSSIPGSGSTFTLYLPLAYTGPASKPHGLAGPSATASSIAQTGLHPIIVPAVRADDLV
                     DDREALQPGESVLLIVEDDPHYARVLLSLARDRGFKALVAKNGTDALDLARRYKPMAI
                     TLDVFLPDMLGWTVLSNLKRDVATRHIPVQIITIEEERQYGLERGAFSFINKPLTTDG
                     LEVAFDRIKRFTEPRQRELLVVEDDSAEQLSIRELIAHNDVVITMAGSGNEALGLLAE
                     RSFDCIVLDLRLPDMTGFELLDRMQADDRLADVPVVVFTGRELSDLEQNELKRKAKSI
                     VLKGVQSPERLFDETALFLHRVIADLPPSKQKLIETLHQSDESLRNRKVLVVDDDIRN
                     IFALNSLLERHNMQVISANNGQEAIRLVETTPELALVLMDIMMPEMDGYETMRRIRTH
                     HEFRRLPIIALTAKAMKGDREKCLEAGASDYVAKPVNTEQLLSLVRMWLHR"
     gene            1666..3366
                     /locus_tag="VNZ06_13995"
     CDS             1666..3366
                     /locus_tag="VNZ06_13995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW21907.1"
                     /translation="MAASIDTAAGDRRADKVNILLVDDQPARLMSYETILSDLGQNLI
                     MARSGLEALERLMQHEFAVVLLDVNMPGMDGFETASMIHEHPRFEKTPIIFVTGVHVS
                     ELDRLQGYQLGAVDYVSIPVVPEILRSKVAVLVELYCQRRELKRLNCSLEEANTALAQ
                     ANSTLQAEKNRELEQLNATLREANRLKDEFLAMLAHELRNPLAPIHNAVQLMRSPVLP
                     AAQLSWARDVIGRQVGHLTRLVDDLLDVSRITRGKINVKRELIEIATVVARAVETVQP
                     MLAGQSHELVMNVEPTPLYVEGDLTRLTQIVGNILSNSIKYTDAGGRIELNVSAVRDR
                     VEIRVRDNGIGIESAALPAVFDLFTQAARSAERAQAGLGIGLALVKRLVELHGGLVEA
                     HSDGLGQGSEFIVWLPRVIREADRSIVPLQPAPTAANCASGSRRILLADDNRDALDSL
                     AELLAIAGHETHKAGDGAQALEAATRLRPDVILLDIGMPGMDGYEVARRIRAQPWGRE
                     PTLVALTGWGQDNDRKRSSEAGFDAHWVKPLELNRLWNLLESLPGADRSAAAARVGTN
                     "
     gene            3380..>4052
                     /locus_tag="VNZ06_14000"
     CDS             3380..>4052
                     /locus_tag="VNZ06_14000"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWW21908.1"
                     /translation="MNSAPIPITGSTAAEAAGLQRSLAPRHVTMISMGGIIGAGLFVG
                     SSAAICAGGPAIVLSYLLAGIMVLMIMRMLGEMAVALPQVRSFSEFARAGLGDWAGFL
                     AGWLYWYFWVVVIPIEAIAGANILHGFLQGSIDLPVWVLGLALMGLMTGVNLMSARSF
                     GEFEFWFASIKVAAIVVFIAIAGAYAFGLTAPHGATLANLTDAGGFMPHGWVAVLAGV
                     TTVFFA"
BASE COUNT          724 a         1191 c         1394 g          743 t
ORIGIN      
        1 cctgatgcag atcttgaaga acctgctgtc gaacgcgttc aagttcaccg accacggcgg
       61 tgtgcgcctg cacgtcggcg tcgcggccgg cggctggagt gcggatcatc cggtgctcag
      121 tcaggcgcag ccggtgatcg agttctcggt cagcgacacc ggtatcggca ttccgccgga
      181 gaagcagcgc atcatcttcg aagcgttcca gcaggccgat gccggtactg cgcgcaagta
      241 cggtggcacc ggcctggggc tggcgatctc gcgcgagctg gcgcacctgc tcggtggcga
      301 gatccgcctg agcagtattc ccggcagcgg cagtaccttc acgctgtatc tgccgctggc
      361 ctataccggg ccggcctcca agccacatgg gctggcgggc ccgtcggcga ccgcgagctc
      421 gatcgcgcag accggtctgc atccgatcat cgtgccggcg gtacgggccg atgatctggt
      481 ggacgaccgc gaagcgttgc aacccggcga gtcggtgctg ctgattgtcg aggacgatcc
      541 gcactacgcg cgtgtgctgc tgagtctggc gcgcgatcgc ggcttcaagg cactggtggc
      601 caagaacggc accgatgcgc tcgatctggc gcgccgttac aagccgatgg cgatcacgct
      661 ggacgtgttc ctgcccgata tgcttggctg gactgtgctc agcaatctca agcgcgatgt
      721 ggcgacccgc cacatcccgg tacagatcat cacgatcgag gaggagcggc agtacggact
      781 ggagcgcggt gcgttctcgt tcatcaataa gccgctgacc acggacggac tggaagtcgc
      841 gttcgatcgc atcaagcgtt tcaccgagcc gcggcagcgc gaactgttgg tggtcgagga
      901 cgactcggcc gagcagctca gtatccgcga actgattgcg cacaacgacg tcgtcatcac
      961 catggccggc agcggcaacg aggcgctggg gctgctggca gagcgcagct tcgattgcat
     1021 cgtgctcgat ctgcgcttgc cggacatgac cggcttcgaa ctgctcgacc ggatgcaggc
     1081 ggatgaccgc ctggcggatg tgccggtggt ggtgttcact ggtcgcgagc tgtcggactt
     1141 ggagcagaac gaactcaagc gcaaggccaa gagcatcgta ttgaaaggcg tgcagtcacc
     1201 ggaacggctg ttcgatgaga ccgcgctgtt cctgcaccgg gtcatcgccg acctgccgcc
     1261 gtcgaagcag aaactgatcg agacgctgca ccagagcgat gagtcgctgc gcaaccgcaa
     1321 ggtgctggtg gtcgacgatg acattcgcaa catctttgcg ctcaacagtc tgctcgagcg
     1381 acacaatatg caggtgatca gcgccaacaa cggccaggag gcaatccgtc tggtggagac
     1441 gacacctgag ctggcgctgg tgctgatgga tatcatgatg cccgaaatgg acggctacga
     1501 gaccatgcgg cgcatccgca cccatcacga gttccggcgg ctgccgatca tcgcgttgac
     1561 ggcgaaagca atgaagggcg accgtgaaaa atgcctggag gcgggcgctt cggactacgt
     1621 agcgaaaccg gtcaataccg agcagctgtt gtcactggtg cgtatgtggc tgcatcgata
     1681 gacacggctg ccggcgacag gcgcgccgac aaggtcaata ttctgctggt tgacgatcag
     1741 ccggcacggc tgatgtcgta cgagacgatt ctgagcgacc tcggccagaa cctgatcatg
     1801 gcgcgctcgg gtctcgaagc gctcgagcgc ctgatgcagc acgagtttgc tgtggtactg
     1861 ctcgacgtca acatgccggg gatggacggt ttcgagaccg cgagcatgat tcacgagcat
     1921 ccgcgcttcg agaagacacc gattattttt gtgaccggcg tgcacgtgtc cgaactggat
     1981 cgcctgcagg gctatcagct cggcgcggtg gactatgtat cgatcccggt ggtgccggag
     2041 atcctgcgca gcaaggtcgc ggtcctggtc gagctgtatt gtcagcggcg cgaactcaag
     2101 cggttgaact gcagcctcga ggaggccaac acggcgctgg cgcaggccaa ttcgaccctg
     2161 caggccgaaa agaatcgcga gctcgagcaa ctgaacgcga ccctgcgcga ggccaaccgg
     2221 ctgaaggatg agtttctggc gatgctggcg cacgaactgc gcaatcctct ggcgccgatc
     2281 cacaacgcgg tacagctgat gcgaagtccg gtgctgccgg cggcgcagct gagctgggcg
     2341 cgtgatgtga tcgggcgtca ggtcgggcat ctgacgcggc tggtagacga cctgctggac
     2401 gtgtcgcgca tcacgcgcgg caagatcaac gtcaagcgtg agctgatcga gatcgcgacc
     2461 gtcgtagcgc gcgcggtgga gaccgtccag ccgatgctgg cagggcagag tcacgaactg
     2521 gtgatgaacg tggagccgac cccgctgtac gtcgagggtg atctgacgcg cctgacgcag
     2581 atcgtcggca acatcctcag caattcgatc aagtacaccg acgccggtgg gcgcatcgag
     2641 ctgaacgtga gcgcggtgcg ggatcgcgtc gagatccgtg tgcgcgacaa cggcatcggc
     2701 atcgaatcgg cagcgctgcc ggcggtgttc gatttgttca cgcaggcggc tcgttcggcg
     2761 gagcgcgcgc aggcgggtct gggcattgga ttggcgctgg tgaaacgtct ggttgagttg
     2821 cacggcggtc tggtggaagc gcacagcgac ggtctgggtc agggcagtga gttcatcgtc
     2881 tggctgccac gcgtgatacg cgaggcggat cgatcgatcg tgccgctgca gcccgcgccg
     2941 accgccgcga actgcgcatc ggggagccgt cgcattctgc tggcggacga caatcgcgac
     3001 gcgctcgaca gtctggccga actgctggcg atagccggtc acgagactca caaggcgggg
     3061 gacggcgcgc aggcgctgga agcggccacg cgtttacggc ccgatgtcat tctgctggat
     3121 atcggcatgc ccggcatgga cggctatgag gtggcgcgcc gcatccgggc gcaaccctgg
     3181 ggacgcgagc cgacgctggt ggcattgacg ggctggggcc aggacaatga tcgcaaacgc
     3241 tccagcgagg ccggtttcga tgcgcactgg gtcaagccgc tggagctgaa ccggctctgg
     3301 aacctgctgg aatcgctgcc aggagcggac cgttccgccg cggcggcgcg tgtcggcaca
     3361 aactgagtac actgcgcgga tgaactccgc gccgatacct atcaccggat ccaccgccgc
     3421 tgaggcggct ggcctgcaac gctccctggc cccgcgtcac gtgaccatga tttcgatggg
     3481 cggaatcatc ggtgcgggac tgttcgtcgg cagcagcgcg gcaatctgcg ccggtggccc
     3541 ggcgatcgtc ctgagctatc tgctggccgg aatcatggtg ctgatgatca tgcgcatgct
     3601 gggagagatg gcggtggcgt tgccgcaggt gcgctccttc agcgaatttg cgcgcgccgg
     3661 cctgggtgac tgggccggat ttctcgcggg ctggctgtat tggtacttct gggtggtggt
     3721 gattccgatc gaggcgatcg ccggggccaa tatcctgcac ggctttctgc agggctcgat
     3781 cgatctgccg gtgtgggtgc tgggacttgc actgatgggt ctgatgacgg gtgtgaatct
     3841 gatgtcagca cgctcgttcg gcgagttcga gttctggttt gcttcgatca aggtcgccgc
     3901 catcgtggta ttcatcgcga tcgcgggcgc gtatgccttc ggactgaccg caccccacgg
     3961 cgcgacgctt gccaatctga cggatgccgg cggattcatg ccgcacggtt gggtcgccgt
     4021 tctggcgggt gtcaccacgg tattttttgc aa
//
LOCUS       DATMRM010000463        10190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3353969, whole genome shotgun sequence.
ACCESSION   DATMRM010000463 DATMRM010000000
VERSION     DATMRM010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10190
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3353969"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(332..781)
                     /locus_tag="VNZ06_14005"
     CDS             complement(332..781)
                     /locus_tag="VNZ06_14005"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HWW21909.1"
                     /translation="MAEPIAEVAEVVRTAVAPVFLLSGVGVTLTMLTNRLARVVDRAR
                     SLESSERDTHEQDLHELQSRLATLARRARLLGRSITLCTLCALLVCMVVVTLFVGAFV
                     NFHLSQVIAVLFVLAMFSFIAGLLLFLREVFVATRALRIGLRGIRPV"
     gene            complement(785..1444)
                     /locus_tag="VNZ06_14010"
     CDS             complement(785..1444)
                     /locus_tag="VNZ06_14010"
                     /inference="COORDINATES: protein motif:HMM:NF017966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0017009 - protein-phycocyanobilin linkage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpcT/CpeT family chromophore lyase"
                     /protein_id="HWW21910.1"
                     /translation="MPKQTVRILKTLLLGLWLLPQTGTVLADSRKKEEAEFVEFLHSL
                     PGDYDNLAQTQDEKDAQHTAVVLSIKPLNLQALGRLVLLARETAANDKRRVLAQRIWT
                     LERDKEHHIVQRVYLFKEPQRWSQSIDNPEVLQALLPDDLQQLFGCELLWTRTDSGFS
                     GSIRPNACRPAAEHEGMLVETSAELKGDDLLLSEQQAGTGRLSAQTDPASSYRFQRRG
                     G"
     gene            complement(1445..2470)
                     /locus_tag="VNZ06_14015"
     CDS             complement(1445..2470)
                     /locus_tag="VNZ06_14015"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21911.1"
                     /translation="MKKLRALVVVHSSLVPPDSLDGYSEKEIDEWRTEYDVVSALRGI
                     GHEVQTLGVLDSLTELRAALIDWRPEVVFNLLEEFNGIVTYDQHVVAFLELMRAPYTG
                     CNPRGLLLSRDKVLCKQLLAFHRIPTPQFAVFRRGQRVSVPRRLRPPLFVKSATEDAS
                     LGIAQASVVDDLAKLRERVQFMHEQIGSDALVEEYIEGRELYVGVLGNERLTRLPVWE
                     MRFGSLPDSLPAIATRKVKWDRAYQKRYEITTCAAQELPPAVLQQLDRLSRRIYRALH
                     LSGYARIDFRVRSDGGVFMLEANANPNLTRDEDFSESAAAAGVDYRELLERIMTLGRN
                     YRVEWRE"
     gene            complement(2467..3501)
                     /locus_tag="VNZ06_14020"
     CDS             complement(2467..3501)
                     /locus_tag="VNZ06_14020"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HWW21912.1"
                     /translation="MPHRPARARKVDWTRLPDEELLRTRICDLRLSLRSSPVQRYVQR
                     LYNELDGRGIRFRPHVWLSEEWFSPDGVPGIAVPFYLAHPRLRRLERKMTREVEGGNG
                     NWLMRILRHEAGHAFDSAFRLRRRAHWRALFGPASTPYRSRYRARPASRHHVQHLGDW
                     YAQSHPTEDFAETFAVWLAPRSGWQRHYKSWPALRKLRFVQQFAGEVGAHRPPVCCRD
                     RVEPLEFNQRTLAQHYRNKLARARTHRGLLADRLLLRVFGSEPDHRALSAASFLRANK
                     QGLLASLIRATGVTRYAAYQVLRTAIHRSERLQLYVRGAQRESLRHTRITLRRLAKLY
                     MRSQGLRLRA"
     gene            complement(3552..4997)
                     /locus_tag="VNZ06_14025"
     CDS             complement(3552..4997)
                     /locus_tag="VNZ06_14025"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HWW21913.1"
                     /translation="MDRPLENSYWVVPGSLLAGEHPSEGDDTETLARVQRLIDAGIDS
                     FLDLTHSGERPEYLRLLPQRVRVQRSPIRDTTVPLNVAEMRAIQSYIADSLAGGRCLY
                     VHCRAGIGRTGTVVGCYLAETGLDGPSALAELNRLWQQSGRAASWPEVPQTLEQADFI
                     RHWPRFRRSQAESAALSSRRDMRERFVGCLLGLAIGDALSARSQHRSPGTFMAITEIA
                     GGGVFELPVGHWSDDTALSLCVAEGLLNCQGWVAGDQAERFGRWQKEGHLTAGGYCTG
                     LTPGTARWLHRQVPGAPRPALASTAINFEAAPLSRVAPLTMFHFAQGVQAVRAAAEAA
                     AALDPAPAVIDASRGLAAMLHSALHGEPLARVLSPRPSLFGEQPLSQALETLLDRDPT
                     RSPEPQAQPALTALSTARWVLATSGAFRSGALRIANMGGDSDVIGAVHGQLAGAFYGQ
                     ASIPGGWLRTLARADRIEAFAVRLYDAGAPP"
     gene            complement(5004..5885)
                     /gene="ttcA"
                     /locus_tag="VNZ06_14030"
     CDS             complement(5004..5885)
                     /gene="ttcA"
                     /locus_tag="VNZ06_14030"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HWW21914.1"
                     /translation="MSGPTPGEPSATVRRLGARLRGLVGKAIEDYRMIEAGDRVMVCL
                     SGGKDSYTLLDMLRSLQRSAPVRFELLAVNLDQKQPGFPGSVLPDYLQSIGMPYHIIE
                     QDTYRVVKRLIPEGKTMCSLCSRLRRGALYRFASEHGITKIALGHHRDDIIETLFLNL
                     FHGGRLKAMAPKLRSQSGRHVVIRPLAYVNERDIARYARGLGFPLIPCTLCGSQPNLQ
                     RSAIKAMLAGWEREQPGRLESIFTALRNVQPATLADSRLFDFSGLRALPADPRGPALT
                     AMSEEEERALEAELSGD"
     gene            complement(5882..7384)
                     /locus_tag="VNZ06_14035"
     CDS             complement(5882..7384)
                     /locus_tag="VNZ06_14035"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HWW21915.1"
                     /translation="MSATPYQQLEQEWRRLHAFLGALSLLRWDAAVMMPRGSSDVRGE
                     QLAALETEYHALLTTPRITRLLDRAQANSQGLEDWQLANLREMRRQRDHAIATPVSLI
                     SRLARATSRAEVQWAEARRQNNFELFAPHLEEVVNLVRDKAALLGQALGLPPYDALMD
                     EFSPGISAADMDAIFKALARRLPGLIREILVLQSTHQPLPLNGKFAPGKQRTLVIEVM
                     RAIGFPFDRGRLDESEHPFTEGVPGDIRVTTRFDGGDLFSGLLGAMHETGHAMYDLGL
                     PQAWRDQPVGRDRGMALEESQSLLLEMIVGRSRPFVEYLRPLLQKHFAVTGPEWEADN
                     IYRKLNYVQRSLIRVDADEVTYPLHILLRYELEKKLLSGELAVREVPEAWNAGMEQRL
                     EVRASGPADGCLQDIHWALGSFGYFPSYALGAVIAAQLWESLHTHNPEVERQIARGEF
                     SGLFGWLREHVHGLGAKVTVNELMKDATDQPLSANALLRYLETKYLEGAA"
     gene            complement(7386..7523)
                     /locus_tag="VNZ06_14040"
     CDS             complement(7386..7523)
                     /locus_tag="VNZ06_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21916.1"
                     /translation="MRSLIVYFTVAVVLAISIGVGFIASDWPRWCHRAQWCAADWSPG
                     R"
     gene            7581..9038
                     /locus_tag="VNZ06_14045"
     CDS             7581..9038
                     /locus_tag="VNZ06_14045"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HWW21917.1"
                     /translation="MPLRICFASAEMSPFAKAGGLADVSGALVKYLHADGHDIRLFMP
                     AYSSIVRKGLEIYPVDFLQNMWVQLGTHRYGFSVLTARLPGSEAFVYLIDCPEAFHRS
                     SLYTTAADEHRRFLVLTHAAFICCQYMRFSPQIMHFNDWHTAAGTLLRRATYAWDRLF
                     EHTRSVLTIHNIGYQGDVSGAVADEILPGGPHQLLNVGGLHLGHINLLRTGIAYADLI
                     TTVSPTYAREIQSPEYGMGLQDLLRARSDSLIGILNGVDYDEWDPRRDPHLPHHYGAN
                     QLGVKAELKQQFLQRLNLASGSRTALIGIVARMATQKGFDLLLEALLPLLQSREFVLV
                     ALGSGEKRYEDFFFSLQRRFPARVHFHRGYSDALAHWIEAASDIFLMPSRYEPCGLNQ
                     MYSLRYGTIPIVRRTGGLADSVQHFDRTSGQGTGVVFNDYDVGGVIWGVNTALDFYKE
                     KALWRRLVQNAMAQDFSWSSQVQHYVDTYEALLRR"
     gene            complement(9054..9509)
                     /locus_tag="VNZ06_14050"
     CDS             complement(9054..9509)
                     /locus_tag="VNZ06_14050"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HWW21918.1"
                     /translation="MPRRWSGMLLVSLTLMLSACASLLPKLQAPQLSVVGIRLGPTGN
                     MQQQPVQLRLHAVNPNDQAIPVRGIVCKLEIESMPFAEGQTEQAFVLPANGEIDFNVN
                     VIANLNSALIALVGGLGSRNVNYRVYGEVHLKGSILHSIPFDQKGRVRL"
     gene            complement(9521..>10190)
                     /locus_tag="VNZ06_14055"
     CDS             complement(9521..>10190)
                     /locus_tag="VNZ06_14055"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HWW21919.1"
                     /translation="DHFAFWRTRLSRQLQRFDLVRIDHFRGLAGYWSVPAGARTARDG
                     RWCPAPGRALFRMLERDFPALPLVAEDLGVITPDVEQLRTEFRMPGMRVLQFGFDGGA
                     DNPHLPHNFTRDVVAYTGTHDNDTTRGWARSLSAEPAQRASFYLRADPDSLEESMQRA
                     VLSSVAQLAIIPAQDLLRLGSEARLNTPGTVSGNWLWRLPPHALGRPLAQQYGALNRI
                     FGRS"
BASE COUNT         1938 a         3349 c         3109 g         1794 t
ORIGIN      
        1 tcgattcagt ttgctgcatg attccttcct gctggaaatc cgcggcgacg tcagtcaacg
       61 attcagttga gcatgaaacc cgcgcagttc cagataacct catcacgaaa atgagtagca
      121 agaaccctgc cgatctggca gacgcgtgat tcgagtgcta acacctctgc caaaacatgt
      181 ccgacatcac aattgtcggg caccgaaagg gcaagtgact gtccagggaa tgacattgcc
      241 ctcagcggcg gcgggcaggc cccaggcgcg gacgagccgg cgagggcatt ggttccggga
      301 atctgaccgc cgcgaacgag gatgcctacc ttcagacggg acgtataccg cgcaaaccga
      361 ttctcagagc acgcgtggca acgaacactt cgcgcagaaa cagcagcaat ccggcgataa
      421 aactgaacat cgccagcacg aatagcacgg cgatgacctg tgacagatga aaatttacaa
      481 aggcgcccac gaacaatgtc accacgacca tgcacaccag cagcgcgcag agggtgcaca
      541 gagtgatcga acgccccagt aagcgcgcac gccgtgccag agttgcgagt ctgctttgca
      601 attcgtgcag gtcctgctcg tgcgtatcgc gctcactgct ctcgagcgat cgtgcccgat
      661 cgaccacacg cgccagacga ttggtcagca ttgtcagcgt gacgccgaca cccgacagca
      721 gaaacaccgg cgcgacggcg gtgcgcacga cctcagcgac ctcggcgatc ggttcagcca
      781 tcgttcatcc accccggcgc tgaaagcggt agctggaggc cggatcggtt tgcgccgaca
      841 gtcggccggt gccggcctgc tgctcgctca gcagtaagtc atcgcctttg agttcggcgc
      901 tggtctccac cagcatgcct tcatgctcgg ccgcggggcg gcaggcgttc ggccgaatcg
      961 agccgctgaa tccgctatcg gtccgggtcc acaacagctc gcagccgaac agctgctgca
     1021 gatcgtccgg taacagtgct tgcagcacct ctggattgtc gatcgactgc gaccagcgct
     1081 gtggctcctt gaacagatag acccgctgaa cgatgtggtg ctccttgtcg cgctcgagcg
     1141 tccagatacg ctgcgccaac actctgcgtt tatcgttggc cgcggtttca cgcgccagca
     1201 gcaccagacg accgagcgct tgcaggttca gcggtttgat cgacagcacc acggccgtgt
     1261 gctgcgcgtc cttctcatct tgcgtctgcg ccaggttgtc gtagtcaccc ggcaggctgt
     1321 gcagaaactc cacaaactcg gcctcttcct ttttcctgct gtccgccagc acggtgccgg
     1381 tctgcggcag caaccacagt cccagcaaca aggtcttcag gatcctgacc gtctgcttag
     1441 gcatttattc acgccactcc acacgatagt tgcgacccag cgtcatgata cgttcgagca
     1501 gctcgcgata gtcgactccc gcagctgcgg ccgattcgga aaaatcctcg tcacgtgtca
     1561 gattgggatt ggcgttggct tccagcatga acacgccgcc gtcgctgcgc acacgaaaat
     1621 cgatccgcgc ataccccgac agatgtaacg cacgataaat gcggcgcgac agccgatcga
     1681 gctgctgcag caccgccggc ggcagctcct gcgcagcgca ggtggtgatt tcgtagcgct
     1741 tttgataggc acggtcccac ttgaccttgc gggtcgcaat ggccggaagc gagtcgggca
     1801 aagagccaaa gcgcatctcc cataccggca gccgcgtgag acgctcgttg ccgagcactc
     1861 cgacatacag ctcgcgccct tcgatgtatt cctcgaccag tgcatccgag ccaatctgct
     1921 cgtgcatgaa ctgtactcgt tcgcgcagct tggcgagatc gtccacgacc gatgcctgtg
     1981 cgattcccag cgacgcatcc tcggtcgccg atttcacgaa cagcggcggg cgcagtcgtc
     2041 gcggcacgct gacgcgctgg ccgcgacgga acaccgcgaa ctgcggcgtt ggaatgcgat
     2101 gaaacgccag caattgcttg cacaacacct tatcgcgcga cagcagcagg ccgcgtggat
     2161 tgcagccggt gtacggcgcc cgcatcagct cgaggaatgc gaccacgtgc tgatcgtagg
     2221 tgacgatacc attgaattcc tcgagcagat tgaacaccac ttccgggcgc caatcgatca
     2281 gcgctgcacg cagctcggtc aatgaatcca gcacgccgag cgtctggact tcgtgtccga
     2341 tgcctcgcag cgcactgacg acgtcatact cggtgcgcca ctcatcgatc tccttttcag
     2401 agtagccatc caggctgtcg ggtggaacca gactcgaatg cacgaccacc agcgcgcgca
     2461 gttttttcat gcccgcaatc gcaatccctg acttcgcata taaagctttg ccaggcgccg
     2521 cagcgtgata cgcgtgtggc gcagtgactc gcgctgcgct ccacgcacgt acaactgcag
     2581 ccgctcgctg cgatggatcg cagtgcgcag cacctgatag gcggcatagc gggtgacgcc
     2641 ggtggctcgg atcagcgatg ccagcaggcc ctgtttgttg gcgcgcagga agctggcggc
     2701 gctcagggcg cgatgatcag gctcactccc aaacacgcgc aacaggagcc ggtcggccag
     2761 caggccgcgg tgcgtgcggg ctcgggccaa cttgttgcgg tagtgctgtg ccagcgtgcg
     2821 ttgattgaat tccaggggtt caacgcgatc gcggcagcat accggcgggc ggtgagcacc
     2881 cacctcgccc gcaaactgct gcacgaaacg cagctttcgc aacgctggcc atgatttgta
     2941 atgccgctgc cagcctgaac gcggcgccag ccagaccgca aaggtctcgg caaagtcttc
     3001 ggtcggatga ctttgcgcgt accaatctcc cagatgctgc acgtgatggc ggctagccgg
     3061 tcgtgcacgg tagcgcgagc gatacggtgt cgatgccgga ccaaacagcg cacgccagtg
     3121 ggcgcgccgt cgcagccgaa acgccgagtc gaacgcatgg ccggcttcgt gccgcagaat
     3181 gcgcatcagc cagttgccgt tgccgccctc cacctcacgg gtcatcttgc gttccaggcg
     3241 cctcaggcgc gggtgcgcga gatagaacgg caccgcgata ccggggactc catccggcga
     3301 gaaccactcc tcggacagcc agacgtgggg acggaaacga atgccgcgcc cgtcgagctc
     3361 gttgtacaaa cgctgcacgt agcgctgcac cggcgagctg cgcagcgaca ggcgcagatc
     3421 gcagatacgc gtacgcagca gctcttcgtc ggggagacgg gtccagtcga ctttgcgagc
     3481 tcgtgccggc cgatgtggca tcgagaaact ctaaccaatc gcgaccgcgc tgtccgccac
     3541 tgcaaggggt cttagggcgg agcgccggca tcgtacaatc gcacggcaaa ggcttcgatc
     3601 cggtcggcgc gcgccagtgt gcgcagccag cctcccggaa tcgaagcctg tccgtagaac
     3661 gcgccggcaa gctggccgtg tacggctccg atcacgtcgc tatctccgcc catgttcgcg
     3721 atccgtagcg ctccggaacg aaaggctccg ctcgtggcca gcacccaacg cgccgtactc
     3781 agggcggtca gagcgggctg cgcctgcggc tcgggcgagc gcgtcggatc ccgatcaagc
     3841 agtgtctcga gcgcctggga gagcggctgc tcgccgaaca gactgggcct ggggctcaag
     3901 acccgagcca ggggttcacc gtgcaaggca ctatgcagca tcgctgccaa tccccgggag
     3961 gcgtcgatga cagccggggc tggatcgagc gcggccgcgg cctcggcggc ggcgcgaacc
     4021 gcctgaacac cctgcgcgaa atgaaacatt gtcagcggcg cgacccgcga cagcggcgcg
     4081 gcctcgaagt tgatggccgt cgaagccagc gctgggcggg gagcgcccgg cacctgccgg
     4141 tgcagccagc gcgccgttcc cggtgtgagg cccgtgcagt aacctcctgc cgtcagatgc
     4201 ccctccttct gccagcgacc gaagcgttct gcctggtcgc cggccaccca gccctggcag
     4261 ttcagcaatc cctcagccac acacagactc agcgccgtat catcgctcca gtggcccact
     4321 ggcaactcga acaccccgcc accggctatc tcggtgatcg ccatgaaagt gcccggggag
     4381 cgatgctggc tgcgagcact cagggcatcg ccaatggcaa gcccgagcag gcaaccgacg
     4441 aagcgctcac gcatgtcacg gcgcgaggac agcgcggcgg actcggcctg gctgcgccga
     4501 aagcgcggcc aatgtcgtat gaaatcagcc tgttcgaggg tctgcggcac ttccggccag
     4561 ctcgccgcac gcccgctctg ctgccacagc cggttgagtt cggccagtgc actgggtcca
     4621 tccagcccgg tctccgccag atagcagccg acgacggtac cggtgcggcc gatcccggcc
     4681 cggcaatgca cgtacaagca cctgccaccg gcgagcgagt cggcgatata tgactggatg
     4741 gcacgcatct cggcgacatt gagggggacg gtggtatcac ggatggggct gcgctgcacc
     4801 cgtacgcgct gcggcaacag tcgcaggtac tcggggcgct cgcccgagtg ggtgaggtcg
     4861 aggaacgaat cgatgccggc gtcgatcagg cgttgcacgc gtgcgagggt ttcggtgtcg
     4921 tcaccctcgg acggatgctc gcccgcgagc aagctccccg gcacgaccca gtagctattc
     4981 tccagcggtc gatccatgga caactaatcg cccgagagtt ccgcctcgag cgcccgctct
     5041 tcttcttcgc tcatcgccgt cagcgccgga ccgcgcggat ccgcaggcag tgcccgcaat
     5101 cccgagaaat cgaacaggcg tgaatcagcc agcgttgccg gctgcacatt gcgcagcgcg
     5161 gtgaagatgg actcgaggcg cccgggttgc tctcgctccc agcccgccaa catcgccttg
     5221 atggcgctgc gctgcagatt gggctgcgag ccgcacagcg tgcatgggat cagcggaaag
     5281 cccaggccac gcgcatagcg cgcaatgtcg cgttcattga cgtacgcgag cgggcggatc
     5341 acgacgtgcc gtccactctg cgagcgcagc ttgggcgcca tcgctttcag tcgtccgccg
     5401 tgaaacagat tcaggaacaa cgtttcgatg atgtcgtcgc ggtggtgtcc caacgcaatc
     5461 ttggtgatgc cgtgttcgga agcgaaacgg tagagcgcac ctcgccgcag ccgcgaacac
     5521 agcgagcaca tcgtcttgcc ttcagggatc agccgcttga cgacgcgata ggtatcctgc
     5581 tcgatgatgt ggtacggcat accgattgat tgcagatagt ccggcagcac gcttcccggg
     5641 aatccgggct gcttctgatc aaggttcacg gcaagcagtt cgaaccgcac cggcgcgctg
     5701 cgctgaagcg atcgcagcat atccagcaat gtgtaggagt ccttgcctcc cgacaggcac
     5761 accatcacac gatcgcccgc ctcgatcatg cgatagtctt cgatcgcctt gccgaccagt
     5821 ccgcgcagcc gcgcgccgag gcgccgcact gtcgccgacg gctcgcccgg ggttggcccg
     5881 ctcacgccgc accctccagg tatttggttt ccagatagcg tagcagtgca ttggccgaca
     5941 gcggctgatc ggtcgcgtcc ttcatcagct cgttgaccgt caccttggca cccaggccat
     6001 gcacgtgctc gcgcagccag ccgaacagcc cgctgaattc tccgcgggcg atctggcgtt
     6061 ccacttccgg attgtgagta tgcaggctct cccagagttg tgcggcaatc accgcaccca
     6121 gggcatagga cgggaagtac ccaaacgagc ccagcgccca gtgtatatcc tgcaggcacc
     6181 catcggccgg gccgctggcg cgcacctcca gccgctgttc catgccggca ttccacgcct
     6241 cggggacctc tcgcaccgcg agttccccgg acagtagctt cttctccagc tcgtagcgca
     6301 acaggatgtg cagcggatag gtcacctcat cggcgtcgac ccggatcagg ctgcgctgca
     6361 cgtagttgag cttgcggtag atgttgtccg cctcccactc agggccggtc accgcgaaat
     6421 gtttctgcaa taagggccgc aggtactcga cgaacgggcg gctgcgccca acgatcatct
     6481 ccagcagcaa cgactgactc tcctccagcg ccatgccgcg atcgcgtccg accggctggt
     6541 cacgccaggc ctgcggcagg cccaggtcgt acatggcgtg tccggtctcg tgcatggcac
     6601 cgagcaagcc tgaaaacagg tcgccgccgt caaagcgcgt ggtgacccga atatcgcccg
     6661 gcacgccttc ggtgaaagga tgttcgcttt catcgagccg accgcggtca aacggaaaac
     6721 cgatcgcccg catcacctcg atcaccagtg tgcgttgctt tcctggcgca aatttgccgt
     6781 tcagcggcag cggctgatgc gttgactgca gcaccaggat ctcgcggatc aggccgggca
     6841 ggcgtcgcgc cagcgctttg aagatggcat ccatgtcagc cgcagaaatc cccggcgaaa
     6901 actcatccat cagcgcgtcg tacgggggca atccgagcgc ctggccgagc agcgctgcct
     6961 tgtcgcgcac cagattcacg acttcctcga ggtgcggcgc aaacaattcg aagttgttct
     7021 gccttcgagc ctccgcccat tgcacttccg cacgcgacgt cgcgcgcgcc aggcgcgata
     7081 tcagcgacac gggagtcgcg atcgcatgat cgcgctgccg gcgcatctcg cgcagattgg
     7141 ccagctgcca atcctcgagc ccctgcgagt tcgcctgcgc ccggtcgagc agacgcgtga
     7201 tgcgcggagt ggtcagcagc gcgtggtact cggtttccag tgccgcgagc tgctcgccgc
     7261 gcacgtcgga actgccccgc ggcatcatca ccgccgcatc ccagcgcagc aaggacaacg
     7321 cgcccagaaa tgcgtgcaga cggcgccact cctgctcgag ctgctgatac ggtgtggccg
     7381 acatgtcagc gtcccggtga ccagtcggcc gcgcaccact gggccctgtg gcaccagcgt
     7441 ggccagtcgg aagcgatgaa ccccaccccg atcgaaatcg cgagcaccac ggccacggtg
     7501 aagtaaacaa tcagcgagcg catatgtgac atgataatga cttcgcaaaa gcgcgtctcg
     7561 cgctgcgcta agatcgaacg atgccccttc gcatctgctt cgcctccgcg gaaatgtcgc
     7621 cgttcgccaa agccggcggg ctcgcggacg tttcaggtgc actcgtcaaa tacctgcacg
     7681 cagacgggca tgacatccgt ctgttcatgc cggcttattc gagcatcgtg cgcaagggac
     7741 tcgagatcta cccggtggac tttctgcaga acatgtgggt gcagctcggt acccaccgct
     7801 acggtttttc ggtactgacg gctcgcctgc caggctccga agcatttgtc tacctgatcg
     7861 attgtcctga agccttccat cgcagctcac tctacaccac ggccgccgac gaacaccgcc
     7921 gcttcctggt cctgacgcac gctgccttca tctgctgtca gtacatgcgg ttctcgccgc
     7981 agatcatgca cttcaacgac tggcacacgg cggccggtac gctgctgcgc cgggcgacct
     8041 atgcctggga ccggctgttc gagcatacgc gctcggtgct gacgatccac aacatcggtt
     8101 atcagggcga tgtcagtggc gcggtggcgg atgagatcct gccgggcggc cctcatcagc
     8161 tgctcaatgt gggtggactg catctcggac atatcaatct gctgcgtacc ggtatcgcct
     8221 acgccgatct gatcacgacc gtcagtccga cctacgcgcg cgaaattcag agccctgaat
     8281 acggtatggg actgcaggat ctgctgcgcg cacgcagcga ttcactgatc ggaatcctca
     8341 acggtgtgga ctacgatgaa tgggatccgc gccgcgatcc gcatctgccg catcactacg
     8401 gggccaacca gctcggtgtg aaagccgagc tgaagcagca attcctgcag cgactcaatc
     8461 tggcgagcgg atcgcgcacc gcgctgatcg gcatcgtggc acgcatggcg acgcagaagg
     8521 ggttcgacct gttgctcgag gcactgcttc cgctgctgca gtcgcgcgag ttcgtgctgg
     8581 tggcgctcgg cagcggcgag aaacgctacg aggatttttt cttcagcctg cagcggcgtt
     8641 tcccggcgcg agtacatttt catcgcggtt acagcgacgc gctggcgcac tggatcgaag
     8701 cggcaagcga catcttcctg atgccttcac gctacgagcc gtgcggactg aaccagatgt
     8761 acagcctgcg ctacggcacg attcccatcg tgcggcgcac cggcgggctt gccgattcgg
     8821 tgcagcattt cgatcgaacc agcggccagg gtaccggtgt ggtcttcaac gactacgatg
     8881 tcggcggcgt gatctggggt gtgaacaccg cgctggattt ctacaaggag aaggcactct
     8941 ggcgtcgact ggtgcagaac gccatggctc aggatttttc ctggagcagc caggtgcagc
     9001 actacgtcga tacctacgaa gcgctgctgc gccgctaaag cgcgcggtcc gacctagagt
     9061 cggacgcggc ccttctgatc gaatgggatc gagtgcagga tcgaaccctt caggtgtact
     9121 tcaccgtaca cgcgatagtt gacgttacgg cttcccagtc cgccgaccag agcgatcaag
     9181 gcgctattga gattggcgat cacgttgaca ttgaaatcga tctcgccgtt ggccggcaga
     9241 acaaacgctt gctcggtctg accttcggcg aacggcatgc tttcgatctc caatttgcag
     9301 acgatgccgc ggactggaat ggcttgatca ttcggattga ccgcgtgcag ccgcagctgc
     9361 acgggctgct gctgcatgtt gccggtaggg ccgagccgga taccgaccac gctcagctgt
     9421 ggcgcctgca gtttgggcag cagcgacgcg caggcgctca gcatcagcgt cagactcact
     9481 agcagcatcc cagaccatcg tcgtggcatg gctttcacgc tcaggaccgt ccgaagatcc
     9541 gattcagtgc accgtactgc tgcgccaacg gcctacccag cgcgtgtggc ggcagccgcc
     9601 acagccagtt gccgctgacg gttcccggcg tgttcagtcg cgcctccgag cctagccgca
     9661 gcagatcctg cgccgggatg atcgccagct gcgcgacgct gctgagcacc gcgcgttgca
     9721 tgctctcttc cagcgagtcc ggatcggccc gcaaatagaa cgatgcacgc tgggccggtt
     9781 cagcgctgag gctgcgggcc cagccacggg tcgtatcgtt gtcgtgtgta ccggtgtacg
     9841 cgaccacgtc gcgggtgaag ttgtgcggca gatgaggatt gtcggcgccg ccatcgaatc
     9901 cgaactgcag cacacgcata ccgggcatgc gaaattcggt gcgcagctgc tcgacatccg
     9961 gggtaatcac acccagatcc tccgccacca gcggcaaggc cggaaaatcg cgctccagca
    10021 tgcgaaatag cgcgcggcct ggcgccgggc accagcgccc gtcgcgcgcg gtgcgtgccc
    10081 cggccggcac cgaccagtag ccggccaacc cgcgaaagtg atcgatgcgc accaggtcga
    10141 agcgttgtaa ctgacgcgac agccgcgtgc gccagaacgc gaaatgatcg
//
LOCUS       DATMRM010000464         8282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_598740, whole genome shotgun sequence.
ACCESSION   DATMRM010000464 DATMRM010000000
VERSION     DATMRM010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8282
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598740"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VNZ06_14060"
     CDS             complement(<1..585)
                     /locus_tag="VNZ06_14060"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HWW21920.1"
                     /translation="MPDSNEPKGFFARLKARLNHGSASIARDLRSLLTGRKIDAGVLE
                     DLETRLIMADVGVEATSDILKDLNRRVSLRELDDVEALLGALRERLTQILKPCEQPLP
                     IDRMRKPYVILIVGVNGSGKTTSIGKLARQLREQHLNVMLAAGDTFRAAAVEQLHIWA
                     ERTGSQFSAQQTGADPGAVIFDSIKAAQARGIDVL"
     gene            637..1212
                     /gene="rsmD"
                     /locus_tag="VNZ06_14065"
     CDS             637..1212
                     /gene="rsmD"
                     /locus_tag="VNZ06_14065"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HWW21921.1"
                     /translation="MKRQERTLRIIAGQWRGRKLRFPEGEIRPTADRVRETLFNWLRT
                     RIQGARCLDLYAGSGALGLEALSRGAGSVVFVEQQRAVANALQALLQQWGAVGAQVHC
                     LQARDYLSRPPGAGRFDVVFLDPPFAAGSSGELLAALSALAGGWLAPRATLYVEHSKS
                     DASLWSELQWRELRAGTAGEVRYHLLEAAES"
     gene            1228..1710
                     /gene="coaD"
                     /locus_tag="VNZ06_14070"
     CDS             1228..1710
                     /gene="coaD"
                     /locus_tag="VNZ06_14070"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HWW21922.1"
                     /translation="MKTNAMYPGTFDPLTRGHEDIVRRAASIFTNVLVAVASNPNKKP
                     MFDIDERCQLARTVLADLINVEITSYSGLTVEFAHQRGYSVVVRGLRAVSDFEFEFQL
                     ASMGRHLQPDIETVFMVPKEKFTFISSTRVREIAELGGDVSSFVHPAVEAAFKQKMKR
                     "
     gene            complement(1738..3453)
                     /locus_tag="VNZ06_14075"
     CDS             complement(1738..3453)
                     /locus_tag="VNZ06_14075"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HWW21923.1"
                     /translation="MSIASRLTATLSAALIAQLACATDAPTPGTLQTGRHAIVVAANP
                     LAAQAGLHVLQQGGSAADAAVAVQVVLGLVEPQSSGLGGGAFISYYDGRTHKISAYNG
                     RETAPAGATDRLFLDDQGKPLSYPAAVLSGRSTGVPGAIAVLHLLQHEHGKLAWKGLF
                     AEGEQLATEGFTVSPRLARMISSRAPQASAPDAMRYFTKPDGARYTTGDVLRNPDYAR
                     TLQLIAAQGPDALLHGSVAQDIVNRVHQGDLPGSLSLDDLAHYRPIESEPLCRDWERY
                     RLCTPPPPGGGIAVLESIGLLEHTDIATRGPADPQGWVELGEAERLMYADRNRYVADP
                     AATAVPINGLLDPNYLRDRAKLIGTHIAESAPEAGHPPGARKVGADHTVEPGGTTNFI
                     IVDKDGNVVCMTTTVESIFGDGRMVDGFFLNNQLTDFNFAPRDSDGGEAANAVGPGKR
                     PRSAMSPIIVLDRQGRFVGAVGSAGGPAIISYVLKTLIGTFDWHLSMQDAISLPNLVA
                     HGANFSGEADKFAPGMLEKLNALGLNIRPGQFEESGLTGVKVDADGTLHGGVDPRREG
                     VALAY"
     gene            complement(3450..4211)
                     /locus_tag="VNZ06_14080"
     CDS             complement(3450..4211)
                     /locus_tag="VNZ06_14080"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21924.1"
                     /translation="MLKTTAAHKVLKTDPIMARLIEKAVHYEPDARLEQSPFEVLARA
                     IAHQQLHATAANNILKRFVALFDETPFPLPRDVIGADQARLRAVGFSYAKIAALKDLA
                     AKALTGVVPGRQGLDSLSDEQIIERLTQVRGVGRWTVEMLLMFHLQRPDILPVDDFGI
                     RNGFRLAYGLKGMPTPKALAEFGERWRPYRSIAAWYLWRANDLAKQNLLFKPRNPPRL
                     ALQKPPAARKNKLARKAAKPRAKAKRAPATRRRRA"
     gene            complement(4214..5029)
                     /gene="mutM"
                     /locus_tag="VNZ06_14085"
     CDS             complement(4214..5029)
                     /gene="mutM"
                     /locus_tag="VNZ06_14085"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWW21925.1"
                     /translation="MPELPEVETTRRGIERAARGLRISEFRIYDHRLRWPIDRSLPQQ
                     LADQQIVEVERRAKYLLLRLTKGTLILHLGMSGSLRLLPGQTERISHDQFDLLLDSGH
                     TLRFNDPRRFGSLHYTIEDPAAHRLLRSLAPEPFDVAFNADYLFRLTRRRSAAIKQVL
                     MNGRLVTGVGNIYASEALFRARVSPRRAARKLTRAECARIVRAVRAALQAAIRAGGTT
                     LRDYVGTDGNPGYFRQKLYVYERDGKACRRCATLVRKITQGQRSTYFCPQCQH"
     gene            complement(5094..5249)
                     /gene="rpmG"
                     /locus_tag="VNZ06_14090"
     CDS             complement(5094..5249)
                     /gene="rpmG"
                     /locus_tag="VNZ06_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HWW21926.1"
                     /translation="MREKVKLLSSAGTGHFYVAMKNKRLHPDKMEVRKYDPVVRKHVA
                     YKETKIK"
     gene            complement(5261..5497)
                     /gene="rpmB"
                     /locus_tag="VNZ06_14095"
     CDS             complement(5261..5497)
                     /gene="rpmB"
                     /locus_tag="VNZ06_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018677334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HWW21927.1"
                     /translation="MTRVCQVTGKRPVTGNTVSHANNRRRRRFLPNLHTQRFWLEGEK
                     RWVTLRISTNAMRTIEKKGLEAVIAEMQARGEKI"
     gene            complement(5646..5996)
                     /locus_tag="VNZ06_14100"
     CDS             complement(5646..5996)
                     /locus_tag="VNZ06_14100"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWW21928.1"
                     /translation="MSYMLLIVEPSGQRDARTLQQGKEVYQRMLDYGESLRVKGVLVD
                     CNSLRSDDAVRLNVRSGKATFTDGPFAEAKELVGGFFLLNCRTRDEALEYARQCPAAE
                     WATIEVRGVGPCYL"
     gene            complement(5996..7300)
                     /locus_tag="VNZ06_14105"
     CDS             complement(5996..7300)
                     /locus_tag="VNZ06_14105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWW21929.1"
                     /translation="MKSNAVLSSWSMGNTDISRSIEAIWRIESAKVIASLARMLRDVG
                     LAEDFAHDALVIALEQWPRVCVPDKPGAWLLTTARHRAIDQLRRRATLTRTHDMLGHE
                     LDMEQEDSNQPFETVAREGIDDDLLRLLFVACHPILSREGRTALTLRLLGGLTTAEIA
                     RAFLVPEPTISQRIVRAKRTLREAGVAFEIPSADERQSRLASVLEVIYLIFNEGYSAT
                     AGNDWMRPALCEEALRIGRILAELAPDDAEAHGLVALMEIQASRFGSRVDARGDPILL
                     FDQDRTRWNRLLIQRGLNALTRVDALKQTRGPYVLQAQIAACHARAAAAGDTDWPAIE
                     GLYAELVHLTASPVIELNRAVAVGMARGPAAGLALADQLSAGGALDSYHLLPSVRADL
                     LQKLGRIAEACSEFERAATLTQNERERALLQRRAAACMRGTV"
     gene            complement(7307..7729)
                     /locus_tag="VNZ06_14110"
     CDS             complement(7307..7729)
                     /locus_tag="VNZ06_14110"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWW21930.1"
                     /translation="MKVMVIVKATRDSEAGVLPAQERLAEMGQFNEQLVKAGVMLAAE
                     GLQSSAKGVRVRFDSGKSTVLDGPFAETKELIAGFWIWQVRSLDEAVEWIRRSPFRKS
                     HEEIEIRRVFEPEDFGDNLTPELRTQEARLRAQSTGRG"
     gene            complement(7888..>8282)
                     /locus_tag="VNZ06_14115"
     CDS             complement(7888..>8282)
                     /locus_tag="VNZ06_14115"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWW21931.1"
                     /translation="LGFTCLQMFAGAVFQLVMSVVDREWDGFEPSHIQLQSVLAVAYL
                     VVFGSLVAVNCYSFLVAHVAAQKVTTYALVNPVIALALGAIVLNERITPSAMVSTLLV
                     LIGVALILFQRHTPQASVASPGLAEQRQ"
BASE COUNT         1499 a         2580 c         2616 g         1587 t
ORIGIN      
        1 cagcacatcg ataccgcgtg cctgcgctgc cttgatcgaa tcgaaaatta cggctcccgg
       61 atcagcgccg gtctgctgcg cgctgaactg cgagccggtg cgttcggccc agatatgcaa
      121 ctgttcgacg gcggccgcgc gaaatgtgtc gccggccgcc agcatcacat tcaggtgctg
      181 ttcgcgcagc tgccgggcaa gcttgccgat gctggtggtt ttaccggagc cgttgacgcc
      241 aacgatcagg atcacgtagg gcttgcgcat gcggtcgatg ggcagcggct gctcgcacgg
      301 tttcagtatc tgcgtcagac gttcccgcag cgcgccgagc agtgcctcca cgtcgtccaa
      361 ctcgcgcaga ctcacgcgac gattcagatc cttcagaatg tcgctggtgg cctcgacacc
      421 gacgtccgcc atgatcagac gggtctcgag atcttccaat acgccggcat cgatcttgcg
      481 cccggtcagc aggctgcgca gatcgcgtgc aatggaggca ctgccgtgat tgaggcgcgc
      541 tttcagacgc gcgaaaaacc ccttgggctc attcgaatct ggcatgaggg cattgtcgca
      601 cggacgcatt cctcagagct gcggtaggct agcgcggtga aacgccagga gcggacgctg
      661 cggatcattg ccggccaatg gcgcgggcgg aagttgcggt ttccggaagg cgaaatccgc
      721 ccgacagccg atcgcgtgcg agagacgctg ttcaactggc tgcgcacccg catccagggc
      781 gcgcgctgtc tggatctgta tgccggatcg ggcgcgctcg gactcgaggc cctgtcgcgc
      841 ggagccggtt cggtggtatt cgtcgaacag cagcgcgccg tggccaatgc gttgcaggcg
      901 ttgttacagc agtggggcgc ggtgggcgcg caggttcact gcctccaggc gcgcgactat
      961 ctctccaggc cgcccggtgc cgggcgcttc gatgtggtgt ttctcgatcc gccgtttgcc
     1021 gccggcagct ccggcgagtt gttggcagcg ctgtcggccc tcgctggcgg ctggcttgcc
     1081 ccacgggcga cactttatgt cgagcattcg aaatccgacg cgtcgctctg gtccgagctg
     1141 cagtggcgcg aactgcgtgc cggtaccgcg ggcgaggtca ggtatcattt gctggaggcc
     1201 gctgagagct aggggggata ggccggcatg aaaaccaacg caatgtatcc aggcaccttc
     1261 gatccgctga cccgcggaca cgaagacatc gtgcgtcgcg cagccagcat ctttaccaat
     1321 gtgctggttg cggtcgcaag caatcccaac aagaagccga tgtttgacat cgacgagcgc
     1381 tgtcagctgg cccgcacggt actggcggat ctgatcaatg tggagatcac cagctattcg
     1441 ggcctgacgg tagagttcgc gcatcagcgt ggctacagcg tggtggtgcg cggcttgaga
     1501 gccgtatcgg acttcgaatt cgagtttcag ctcgccagca tgggccggca tctgcagccg
     1561 gacatcgaga cggtgttcat ggtgccaaag gagaagttca ccttcatctc ctctacccgt
     1621 gtccgggaga ttgccgaact gggcggtgac gtcagctcat tcgtgcaccc agccgtcgag
     1681 gccgccttca agcagaagat gaagcgctga tcgcggggcg aagccgcgcg catcgaatca
     1741 atacgccagt gccacgcctt cacgccgcgg atccactccg ccgtgcaagg ttccatccgc
     1801 atccactttg acaccggtca gtccggattc ctcgaactgt ccgggacgga tattcagacc
     1861 gagcgcattc agcttctcga gcatgcctgg cgcgaacttg tccgcttcgc ccgagaagtt
     1921 ggcgccgtgc gccacgaggt ttggcaggct gatcgcatcc tgcatcgaca ggtgccagtc
     1981 gaacgtcccg atcagcgtct tcagcacata ggagatgatc gccggccctc ctgcggatcc
     2041 cacggcgcca acgaaccgtc cctggcgatc cagcacgatg atcggggaca tcgccgagcg
     2101 tggacgcttg cccggtccca cggcgttggc cgcttcccca ccgtcactgt cgcgcggcgc
     2161 gaaattgaaa tcagtcagct ggttgttgag gaaaaagcca tccaccatgc gaccgtcgcc
     2221 gaagatgctt tccacggtcg tggtcatgca gaccacattg ccgtctttgt cgacgatgat
     2281 gaaattggtg gtgccgccgg gttcgacggt gtgatcggcg ccgaccttgc gtgctccggg
     2341 tggatgaccg gcctcggggg cggactccgc gatgtgagtg ccgatcagct ttgcgcggtc
     2401 gcgcagatag ttcgggtcga gcaggccatt gatcggcacc gcggtggcgg ccggatctgc
     2461 cacatagcga ttgcggtcgg cgtacatcag ccgctcggct tcacccagct caacccagcc
     2521 ctgcggatcg gcaggaccgc gcgtggcgat gtcggtgtgc tcgagaagtc cgatcgattc
     2581 gagcaccgcg atgccaccgc ccggtggtgg tggagtgcac agccgatacc gctcccagtc
     2641 gcggcacagg ggttccgatt cgatcggcct ataatgcgcg agatcatcga gcgacagtga
     2701 gcctggcaga tcgccttggt gcacgcgatt gacgatgtcc tgcgcgaccg aaccgtgcag
     2761 cagtgcatca ggtccctgcg cggcgatcag ctggagcgtg cgggcgtagt cgggattcct
     2821 cagtacgtcg ccggtggtat agcgtgcgcc gtccggcttg gtgaaatagc gcatcgcgtc
     2881 aggtgccgac gcctgcggag cgcgactcga tatcatgcgc gcgagccgtg gactgacagt
     2941 gaagccttcg gtggcgagtt gttcgccttc agcaaagagg cccttccacg cgagcttgcc
     3001 gtgttcgtgc tgtagcaggt gcagcaccgc gatggcgccg ggcacaccgg tcgaacgccc
     3061 gctcaacaca gcggccggat aagacaacgg tttgccctga tcgtccagaa acagtcgatc
     3121 ggtcgcgccg gcgggcgcgg tttcgcggcc gttgtatgcg gaaatcttgt gtgtacgccc
     3181 gtcgtagtag ctgatgaatg caccgccgcc caggccagaa ctctgcggct cgaccagacc
     3241 cagcacgacc tgcaccgcga ccgcggcatc tgccgccgat ccgccctgct gcagcacgtg
     3301 cagaccggct tgggcggcca gcggattggc agccaccacg atcgcgtggc gcccggtctg
     3361 cagagtgccc ggcgtcggtg cgtcggtcgc gcaggccagc tgcgcgatca gtgcagcgct
     3421 caaggtggcg gtcaaccgcg aggcgatgct catgcgcgcc tccggcgcgt ggcaggcgcg
     3481 cgtttggcct tcgcacgagg tttcgccgct ttgcgcgcga gtttgttctt tcgcgccgcg
     3541 ggcggcttct gcagggccag tcggggcgga ttgcgcggtt tgaatagcag gttctgcttt
     3601 gccagatcgt tggcgcgcca caaataccag gccgcgatgc tgcgataggg cctccagcgc
     3661 tcgccgaatt cagccagtgc cttcggtgtc ggcatgccct tcagtccgta ggccaggcga
     3721 aaaccgttgc ggataccgaa atcgtccacc ggcagtatgt ctggccgctg caggtgaaac
     3781 atcagcagca tctcgaccgt ccagcgtccg acgccacgca cttgcgtcag gcgctcgatg
     3841 atctgctcat cgctgagcga atcgagccct tggcgaccag gcacgacgcc ggtcagtgct
     3901 ttggccgcga gatccttgag tgccgcgatc ttggcgtatg agaagccgac ggcgcgcagg
     3961 cgtgcctggt cggcaccgat gacatctcgt ggcaagggga atggcgtttc gtcaaacagc
     4021 gccacgaagc gcttcaggat gttgttggcg gccgtggcgt gcagttgctg atgcgcaatg
     4081 gcgcgcgcga gcacctcgaa cggtgactgc tcgagccgcg cgtcgggttc gtagtgaacg
     4141 gctttctcga tcaggcgcgc catgatcgga tcggttttca acactttgtg ggcggcggtg
     4201 gttttgagca tcgtcaatgc tgacactgtg gacagaagta agtagagcgc tggccctggg
     4261 tgatcttacg cacgagcgta gcgcaccggc gacaggcctt gccatcgcgt tcgtagacgt
     4321 agagcttctg acggaagtaa cccggattgc cgtcggtgcc gacgtagtcg cgcagcgtag
     4381 tgccgccggc acgaatcgcg gcctgtaatg ctgcgcgtac cgcgcgcacg atgcgcgcgc
     4441 actcggcgcg cgtcagtttg cgcgccgccc ggcgtgggct gacgcgagcg cggaacaggg
     4501 cttcactcgc gtagatgttg cccacgccgg ttaccagccg gccattcatc agcacctgtt
     4561 tgatggcggc actgcgccgg cgcgtgaggc gaaagaggta gtcggcattg aaggcgacat
     4621 cgaatggctc cggtgccagt gagcgcagca gcctgtgtgc ggcagggtct tcgatcgtgt
     4681 agtgcagact gccgaagcgg cgcgggtcgt tgaaacgcag cgtgtgcccg gaatccagca
     4741 gcagatcaaa ctggtcatgg ctgatacgct cggtttgccc cggcagcagt cgcaaacttc
     4801 ccgacatgcc cagatgcagg atcagggtgc ctttggtgag gcgtagtaac aggtatttgg
     4861 cgcgtcgttc cacttcgacg atctgttgat cggccagctg ttgcggcaga gaacggtcga
     4921 ttggccagcg cagccggtga tcgtagatac gaaactcgct gatgcgcaga ccccgcgctg
     4981 cgcgttcgat gccgcggcgg gtggtttcga cttctggcaa ctctggcatg catcgacctt
     5041 aaacaaaaaa cccgcctcgc ggcgggtttt actgcgatca gacaggcgcg gcgctacttg
     5101 atcttcgttt ccttgtaggc gacatgcttg cgcaccaccg ggtcgtactt gcgcacttcc
     5161 atcttgtccg gatgcagacg cttgttcttc atcgcgacgt agaagtggcc ggtgccggcc
     5221 gatgacaata gtttgacctt ctcacgcatg attcaaaccc tcaaatcttc tcaccacgcg
     5281 cctgcatttc ggcgatcacg gcttcaagac ccttcttctc gatggtgcgc atcgcgttgg
     5341 tcgaaatacg cagggtgacc cagcgcttct cgccctcgag ccagaatcgc tgcgtatgca
     5401 gatttggcag gaaacgacgc cgacgccggt tgttggcgtg agagaccgta ttacccgtca
     5461 ccggacgctt gccggtcacc tgacagacgc gagtcataag ctcaaaacct caacaatcaa
     5521 tacgttacgc gcacatggcc cgtcgccgga agcgacgaaa ggggcgcttt ataccagtac
     5581 ctcagcgact taggcaagcg aggtgagtgc ggcggtgaaa ttggctggaa tttttaagat
     5641 atatcttaca ggtaacaggg tcccacccca cgaacctcga tcgtcgccca ctcggccgcc
     5701 gggcattgcc gcgcatattc gagcgcttcg tcgcgggtcc ggcagttcag cagaaaaaac
     5761 cctcccacca gctccttggc ctcagcgaac ggtccgtccg taaaggtggc tttgcccgac
     5821 cgaacgttca ggcgcaccgc atcgtcgctg cgcagtgagt tgcagtccac caaaacaccc
     5881 ttaacgcgta acgattcgcc gtagtccagc atacgttggt agacctcttt gccctgctgc
     5941 agcgttcgtg cgtcacgctg gccactgggt tcgacgatca gaagcatgta cgacatcaga
     6001 cagtccctct catgcaggcg gctgcgcgac gctgcagcag cgcccgctca cgttcattct
     6061 gggtcaacgt cgcggcgcgt tcgaattcac tgcacgcttc cgcgatccga ccgagctttt
     6121 gcagcagatc cgcgcgcacg ctcggcagca gatgatagga gtccagggcg ccgccagcgc
     6181 tgagctgatc ggccaatgcc agccccgcgg ccggaccgcg cgccatgccg accgcgaccg
     6241 cgcgattcag ttcgatgacc ggcgaggcgg tcaggtggac gagctctgca tacaaccctt
     6301 cgatagccgg ccagtcggta tcgccggcag cggctgcgcg tgcatgacag gccgcgatct
     6361 gcgcctgcag tacatacggt ccgcgcgttt gcttgagtgc atcgacgcga gtgagtgcat
     6421 tcagccctct ttgtatcagc aggcgattcc agcgtgtgcg gtcctgatcg aacaagagta
     6481 tcggatcgcc ccgggcgtcc acgcgcgagc caaagcgcga ggcctgtatc tccatgagtg
     6541 caaccaatcc atgagcttcc gcgtcgtcgg gtgccagttc cgccaggatg cgtccgatgc
     6601 gcagcgcttc ttcgcacaat gcggggcgca tccagtcgtt gccggccgtg gctgaatagc
     6661 cttcgttgaa gatgagatag atgacttcca gcaccgaggc caggcgactc tggcgctcgt
     6721 cggcagaggg aatttcaaac gcgacacccg cttcgcgcag ggtgcgctta gcgcgcacga
     6781 tccgctgaga aatggttggc tccggtacca ggaaggcgcg cgcgatctcg gcagtggtga
     6841 gtccaccgag cagtcgcagc gtcagcgcgg tgcgaccttc gcgcgagagg atcggatggc
     6901 aggccacgaa cagcaggcgc agcaggtcat catcgattcc ctcgcgcgcg accgtttcga
     6961 acggttgatt gctgtcctct tgttccatgt caagctcgtg tccgagcatg tcgtgggtgc
     7021 gtgtcagcgt ggcgcgacgg cgcagctgat cgatcgcgcg atgccgtgcc gtggtgagca
     7081 gccaggcgcc gggtttgtcc ggaacgcaga cgcgcggcca ttgctcgagc gcgatcacca
     7141 gcgcgtcgtg ggcaaagtcc tcggccagcc cgacgtcgcg cagcatgcgt gccagcgagg
     7201 cgatcacctt tgccgattcg atccgccaga tggcttcgat cgaacgcgaa atgtccgtat
     7261 tgcccatgct ccacgatgac agcaccgcgt tggatttcac gcaagctcaa ccccgtcctg
     7321 tcgactgagc gcgcagccgc gcttcctgag tacgcagctc tggcgtgagg ttgtcgccga
     7381 aatcctcggg ttcgaacacg cggcggattt cgatctcctc atgggacttg cggaacggtg
     7441 agcggcggat ccattcaacg gcctcatcga gcgatcgcac ctgccagatc caaaagcccg
     7501 cgatcagctc cttggtctcg gcgaacggcc catcgagcac agtggacttg ccactgtcaa
     7561 agcgcactcg tacgcccttg gcgctcgact gcagtccttc cgccgccagc atcacgccgg
     7621 ccttgaccaa ctgctcgttg aactgcccca tctcggcaag ccgctcctga gcgggcagca
     7681 ctccggcctc tgaatcccgc gttgccttca caatcaccat gaccttcatg tcgttgctcc
     7741 tctgactgga ttttcttggg tccaccgtca tacgacgaac gtgcgaccac ggtttcgaca
     7801 ccatcctaga gcatcagttg gagcatcagt ggcgactctc gggcatcgcg acttttccag
     7861 aagtatcctt aagttacggg ctagccatca ctggcgctgt tcggccagcc cggggctggc
     7921 aaccgatgct tggggagtgt gtcgctggaa caaaatcagg gccacaccga tgaggaccag
     7981 tagcgtggac accatcgcgc ttggcgtgat ccgctcgttg agcacgatcg cacccaacgc
     8041 gagcgcgatg acaggattga cgagcgcata ggtggtgacc ttctgggcag ccacgtgcgc
     8101 caccaggaag gaatagcaat tgacggcgac caggctgccg aacaccacca gatacgccac
     8161 cgcaagtacg gactgcagtt gaatgtgtga tggctcaaag ccgtcccatt cccggtcgac
     8221 caccgacatc acgagctgaa acactgcgcc ggcgaacatc tgcaggcagg tgaagccgag
     8281 ca
//
LOCUS       DATMRM010000465         4008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3354448, whole genome shotgun sequence.
ACCESSION   DATMRM010000465 DATMRM010000000
VERSION     DATMRM010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3354448"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VNZ06_14120"
     CDS             complement(<1..613)
                     /locus_tag="VNZ06_14120"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21932.1"
                     /translation="MDSRVRIKDHWNEQRIFSQRTLGAFVIIAALVLTLIGRLTYLQV
                     FRHDYYLNLSQGNRVRLDPIPASRGLIRDRNGAILADNEPAYQLELIREQVPDLNNTL
                     KRLSTLGLIDPDALEDTRRVIFSRRSFDVVPVRLRLTDEEIGRFAVHRYQFPGVDLAT
                     RQTRHYPYGELAVHALGYVSAISDQDLEHIDRNAYAGTTLIGKL"
     gene            complement(643..1113)
                     /gene="mreD"
                     /locus_tag="VNZ06_14125"
     CDS             complement(643..1113)
                     /gene="mreD"
                     /locus_tag="VNZ06_14125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HWW21933.1"
                     /translation="MSPAYPRLTIFTTSLIALLLTVAPLPTWGAIVRPAFLVLTVLYW
                     SIMIPRVGLLLAFVGGLAIDVFQGSLLGEHAFALVFVSYLGIRLNLLVRAKPLFEQSL
                     FVFAALMVYEFLLWVIDGWSGHPLTTPARWVHTLTGGLIWPLVVGILGRFQPTR"
     gene            complement(1110..2048)
                     /gene="mreC"
                     /locus_tag="VNZ06_14130"
     CDS             complement(1110..2048)
                     /gene="mreC"
                     /locus_tag="VNZ06_14130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HWW21934.1"
                     /translation="MAVFGTDVDRPLYGRGPSAGLRCTGYAALSIALMYYDQHGHLAQ
                     RLRFALDAVAYPVQVAVSSPGAGWRYLTQNFHSRDELRTENQQLKARVTDLELRVIRE
                     AALEQENNELRALRQSLPPFIKHWQLAEIISVETNPLRQRVVINKGTRDGVVASQAVI
                     DGSGVLGQVARVGPFSAEVILVTDPEHAIPVEVTRNSLRSIAVGSGNSGELALPYLAV
                     NSDVKSGDLLVSSGLGGVFPAGLPVAKISGVRREANQLLAQVRAQPLATVDKDREVIL
                     LDFEPSHPAAPASNPPIDPEPQPKAKPAPKILDPGL"
     gene            complement(2068..3111)
                     /locus_tag="VNZ06_14135"
     CDS             complement(2068..3111)
                     /locus_tag="VNZ06_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019408857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWW21935.1"
                     /translation="MFKRLRGYFSSDLSIDLGTANTLIYVRDKGIVLNEPSVVAVQDE
                     PSRGGKIIVAVGQDAKSMLGRTPGHITAVRPMKDGVIADFTYTEKMLQFFINKVHGNR
                     FLKPSPRVLVCVPFGSTQVERRAIRESAEGAGARNVAIVSEPMAAAVGAGLPVQEARG
                     SMVLDIGGGTSEVAVLSLNGIVYANSARIGGDRFDDAIMNYVRRNYGILIGESTAERI
                     KIEIGSAYPGQQVRELSVKGRNLSEGVPRSFTLNSNEILEALQEPLQGIVSSVKQALE
                     QTPPELGADVAERGIVLTGGGALLRDIDKLLMEETGLPVVVADDPLTCVARGGGRILE
                     LMDQMGPGHFGLE"
     gene            3237..3527
                     /gene="gatC"
                     /locus_tag="VNZ06_14140"
     CDS             3237..3527
                     /gene="gatC"
                     /locus_tag="VNZ06_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004574467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HWW21936.1"
                     /translation="MSLNRRDIENIALLARLALTEAEIPVYVESLSRIMGLIGELDKA
                     DTSQIEPMAHPLPGQRQRLRADTVTETDQHALYQANAPQVEAALYLVPRVIE"
     gene            3524..>4008
                     /locus_tag="VNZ06_14145"
     CDS             3524..>4008
                     /locus_tag="VNZ06_14145"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWW21937.1"
                     /translation="MSELHHKTVAELATALRAREVSSVELTRACLQRIEQHQGELNAF
                     ISVTAHAALAQAAEADQRRAAGKAGPITGVPIAHKDIFCTRGEKTTCGSRMLANFVSP
                     YDATVVERLRAAGVVMLGKTNMDEFAMGSSNETSYFGPVRNPWDTARVPGGSSGGSAA
                     AV"
BASE COUNT          768 a         1262 c         1281 g          697 t
ORIGIN      
        1 ccagcttgcc gatcagcgtg gtgccggcgt aggcattgcg gtcgatgtgc tcaagatcct
       61 ggtcgctgat cgcactcacg taaccgagcg cgtggacggc aagctcgcca taggggtagt
      121 gccgcgtctg acgcgtcgcc agatcgacgc ccgggaactg gtaacgatgc accgcgaagc
      181 gtccgatctc ctcatccgtg agtcgcaggc gtaccgggac gacgtcaaag ctgcgccgcg
      241 agaaaatgac gcggcgggta tcctcgagcg catccggatc aatgagcccg agcgtactga
      301 gccgcttgag cgtgttgttc aggtcgggca cctgctcgcg aatcagctcg agctgatagg
      361 caggttcgtt gtcggccagg atggcgccgt tgcgatcgcg tatcaggccg cgactggccg
      421 gaatgggatc caggcgcaca cggttgccct ggctgagatt caggtagtag tcgtggcgaa
      481 agacctgcag gtaggtcagc cggccgatca gggtcaggac cagggcggcg atgatgacga
      541 acgcgccgag cgtgcgctgc gagaagatgc gctgctcgtt ccaatggtcc ttgatgcgaa
      601 cgcgagagtc cacgagtgct tgacgccgcc tgcctttgac tactagcgag tgggctggaa
      661 acgaccgagg atgccgacga cgagcggcca gatcagacca ccggtcaagg tgtgtaccca
      721 gcgcgccggg gtcgtcagcg gatgtccgct ccaaccatca atgacccaca gcagaaattc
      781 ataaaccatc agcgctgcga acacaaacag cgattgctca aagagcggct tggctcgcac
      841 taagagattg aggcggatcc ccaggtagct cacgaacacc agtgcgaacg cgtgctcgcc
      901 cagcagcgag ccctggaaca cgtcaatcgc cagtccgccg acgaacgcca gcagcaggcc
      961 aacgcgtgga atcatgatcg accagtagag caccgtcagc accaagaagg cgggtcgcac
     1021 gatcgcgccc caggtgggaa gcggggcgac cgtgagcagc agcgcgatca ggctggtcgt
     1081 gaaaatcgtc agacgagggt aggcagggct cacaggccgg gatccagaat cttaggcgca
     1141 ggcttggcct tgggttgagg ctcaggatcg atcggcgggt tgctggctgg tgccgccgga
     1201 tgagagggct cgaagtcgag cagaatgact tcgcggtcct tgtccaccgt tgccagcggc
     1261 tgtgcgcgca cctgcgccag cagctgattg gcttcgcgtc gcacgccgct gatctttgca
     1321 acgggcagtc ccgccgggaa cacgccgccc agacctgaac tgaccaacag gtcgccgctc
     1381 ttcacatccg agttcaccgc cagatacggc agtgccagct cgccgctgtt gccagagccg
     1441 accgcgatcg agcgcaggct gttgcgcgtg acctccaccg gaatcgcgtg ttcggggtcg
     1501 gtcaccagaa tgacctcggc gctgaacggt cccacgcgtg cgacctgacc gagcacgccg
     1561 ctgccgtcaa tcacggcctg actggcgacg acgccatcgc gcgttccctt gttgatcacg
     1621 acccgctggc gcagtggatt ggtctccacg ctgatgatct cggccagttg ccagtgcttg
     1681 atgaacgggg gcaaggattg ccgcagagct cgcagctcat tgttttcctg ctccagggcg
     1741 gcctcgcgga tgactcgcag ctcaagatcg gtgacccgcg ctttcagctg ctggttttcc
     1801 gtgcgcagct cgtcgcgact atgaaagttc tgggtaagat agcgccagcc ggcgccaggg
     1861 gaactgaccg ccacctgcac cgggtaggcg accgcgtcca gggcaaaacg cagtcgctgc
     1921 gccaggtggc cgtgctggtc gtagtacatc agggcgatcg acagcgccgc gtagccggta
     1981 cagcgcagac cggcagacgg tccacgcccg tacagaggtc gatcgacatc agtaccgaag
     2041 accgccatag ctgcggccta ctcgccgcta ctccagcccg aaatgtccag gccccatttg
     2101 atccatgagt tcgagaatgc gaccgccacc gcgtgcgacg cacgtcagcg ggtcatcggc
     2161 gaccacgacc ggcagtccgg tctcttccat cagcagtttg tcgatgtcac gcagcagcgc
     2221 gccgccaccg gtcagaacaa tgccgcgctc ggcgacgtcc gcacccagtt cgggcggagt
     2281 ttgttcaagg gcctgcttga cggaactcac gatcccctgc agcggttcct gcagggcctc
     2341 gaggatctcg ttgctgttga gcgtgaaact gcgcgggaca ccttccgaca gattgcggcc
     2401 tttgactgac agttcccgca cctgctggcc gggataggcc gatccgatct cgatcttgat
     2461 gcgctcggcg gtggactcgc cgatcagaat tccatagttg cggcgcacgt agttcatgat
     2521 cgcgtcgtca aagcgatcgc caccgatgcg cgccgagttg gcgtagacga tgccgttcag
     2581 cgacagcaca gccacctcgc tggtgccgcc accgatatcc agcaccatcg acccgcgtgc
     2641 ctcctgcacc ggcagaccgg cgcctaccgc ggcggccatt ggctcgctga cgatggcgac
     2701 gttgcgtgcg ccggcgcctt cggccgattc gcggattgcg cggcgttcga cctgcgtcga
     2761 accgaacggc acgcacacca gcacgcgcgg cgagggcttc aggaagcggt tgccgtgcac
     2821 cttgttgatg aagaactgca acatcttttc cgtatacgtg aagtcggcga tgacgccgtc
     2881 cttcatcgga cgtactgcgg tgatgtggcc cggtgtgcga ccgagcatgc tcttggcgtc
     2941 ctgaccgact gccacgatga ttttgccgcc gcgcgagggt tcgtcctgta ccgctacgac
     3001 cgagggctcg ttgagcacaa tgcccttgtc gcgcacgtag atcagcgtgt tggcagtgcc
     3061 gaggtcgatc gacagatcac tggaaaaata gccgcgcagg cgtttgaaca tgagagcggc
     3121 ggaccttctt cataacgtct taaaatcggg cgctaatcta gcaaccgaca ggacattcca
     3181 caaggcgagg tgtatgattt cgcgctgttt tttctgcgaa atttgaacga atccacatga
     3241 gtctcaaccg acgcgacatc gaaaatatcg cgctgctcgc gcgtctcgcg ctcactgaag
     3301 cggagattcc ggtctacgtc gagtcacttt cgcgcatcat gggattgatc ggcgaactcg
     3361 acaaggccga taccagtcag atcgagccga tggcgcatcc gctgcccggg cagcgccagc
     3421 gtctgcgcgc tgatacggtg accgagaccg accagcatgc gctgtaccag gccaatgcac
     3481 cacaggtcga ggcggccctg tatctggtcc cacgagtgat cgaatgagtg agctgcatca
     3541 caaaacggtc gcggaattgg ccaccgcgct gcgcgccaga gaggtttcca gcgtcgagct
     3601 gacccgcgcc tgcctgcagc gcatcgagca gcaccagggc gagctcaacg cattcatcag
     3661 cgtgacggcg cacgcggcac tcgcccaggc ggccgaagcc gatcagcgcc gggcggctgg
     3721 caaggccggg ccgatcaccg gcgtgccgat cgcgcacaag gatatcttct gtacccgcgg
     3781 cgagaagacc acctgcggct cgcgcatgct ggccaacttc gtgtcgccgt acgacgccac
     3841 ggtggtcgaa cggctgcggg cggcgggcgt ggtgatgctg ggcaagacca atatggacga
     3901 gttcgccatg ggatcgtcca acgaaaccag ctacttcggc ccggttcgca acccctggga
     3961 cacggcgcgt gtgccgggcg gctcttccgg cggctcggcg gcggcggt
//
LOCUS       DATMRM010000466         5535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1518637, whole genome shotgun sequence.
ACCESSION   DATMRM010000466 DATMRM010000000
VERSION     DATMRM010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518637"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VNZ06_14150"
     CDS             complement(<1..212)
                     /locus_tag="VNZ06_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWW21938.1"
                     /translation="MADPRARRQSRYIRVDLRNVRLARDGKPVLRDVSLSIRPGQRWV
                     LIGPNGAGKTQLLKLLAGDVWPTPSRG"
     gene            complement(503..1324)
                     /locus_tag="VNZ06_14155"
     CDS             complement(503..1324)
                     /locus_tag="VNZ06_14155"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWW21939.1"
                     /translation="MLRPTNRLAVSVRADAVGEHRHQSAPPAAQATRQSLSLTGEIAA
                     ILLLIYRMLAAGGSLRNRIAHMHFIPVRTREGIRLILLEVTRSLHQYLVCLVLTNTLL
                     GLAVWGAFALTGVHYALAWGFAAAVLHFVPYVGPAAIAIGSTVFAAVQFDSLANGMII
                     GGMTVLLSTLICVVLQTWLTGRSVRMSTIAVFVSLLFWSWIWGLPGLVLGIPIMIVIK
                     AICSQIPQMGWFDALLAQRTRRRASPASRANLSPVPRDGAGASEFALPEVTASRR"
     gene            1381..1677
                     /locus_tag="VNZ06_14160"
     CDS             1381..1677
                     /locus_tag="VNZ06_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21940.1"
                     /translation="MSEPTDRNANDTVQGEGDYDAARRYRDKVADFIKRSDVEDLARK
                     SAPLSAREARDDALADERARGRSKGDIPADIGVMYPHQGSSAHAETSTDPQRKK"
     gene            complement(1715..2329)
                     /locus_tag="VNZ06_14165"
     CDS             complement(1715..2329)
                     /locus_tag="VNZ06_14165"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWW21941.1"
                     /translation="MTGTEREQPYPFLSEIAELRRRARQHIADGAVTVNYAADREVVV
                     RLLNEALATELVCVLRYRRHYFMASGSLAEAIKDEFLKHSQEEQQHADQLAERIVQLG
                     GQPNFSPQGMLDRSHSEYVDGESLAEMVEEDLIAERIAIESYREIIQYIGEKDTTTRR
                     LFESILAIEEEHAEELASMRQDLLRHGRGSAGNNAIPIQSGELQ"
     gene            complement(2361..2549)
                     /locus_tag="VNZ06_14170"
     CDS             complement(2361..2549)
                     /locus_tag="VNZ06_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21942.1"
                     /translation="MRALTGQHGRNMLRNAALALAMSRPKTAWRLAGLAPLLRPMILR
                     YLAARILRPRKPSFSVTN"
     gene            complement(2677..3102)
                     /locus_tag="VNZ06_14175"
     CDS             complement(2677..3102)
                     /locus_tag="VNZ06_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21943.1"
                     /translation="MLEALQRLAPAVLRHLIAYGELLCEEAGDALRQSRRRALGLAVT
                     VTAGVMALSLACVWAIAASWDGPNRLAVVGMLCFSFVIVALAGAAYARGAVQPGRPVP
                     FERLRAEWQADMRELAQLDPSLGILTRAHASAPQSLNGD"
     gene            complement(3122..3508)
                     /locus_tag="VNZ06_14180"
     CDS             complement(3122..3508)
                     /locus_tag="VNZ06_14180"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HWW21944.1"
                     /translation="MNANLNDTAESLHRAGKDATADFRRRGANIRDYASDELRAFLAD
                     VEDLVKKVANVGDADVARLRARVSGAIGDVRQTIGDTANSLRDRARMAATVTDDYVHD
                     SPWTAIGLAATVGVIVGVGVAAASRR"
     gene            3683..5101
                     /locus_tag="VNZ06_14185"
     CDS             3683..5101
                     /locus_tag="VNZ06_14185"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HWW21945.1"
                     /translation="MIRSGRISRALTLSVAFLLLVALVVIAEIGQNGQKEIYRLINLS
                     QERQVLLAELVSELSDAEAGQRGFLLTNDQNYLMPYQIAHDRVEPTLNKLTDLFRDND
                     KLLMSREQRDTVRHLRTLVGTGLGELAASLALYTTQGPEQALALVRTDLGSRTMGEIR
                     AEALKLRNFERDGVTAALARAERLRVISRLLMGGVVILNVGLMVMAAQLVARQARRRA
                     ELTEQLATENEELERRVRRRTAELSALSSHLQQLSEKEKASLARELHDELGGLLIAVK
                     MDVSWLQKRWPNSDPDIQARWTRVLKVLDDGVDFKRRIVESLRPTLLDNMGLLPAVRW
                     ITQETCSRAGLQCAEIYPEHEPELIDDAAIMVFRLVQESLINIVKHARATQVKVEIAL
                     TDSEMTVKIEDNGVGIDSDRREAVGSHGLATMRHRVRSFGGTFDIDAPPQGGTCIQAR
                     ILLRDILKTPRPNLLTVAATVA"
     gene            complement(5109..>5535)
                     /locus_tag="VNZ06_14190"
     CDS             complement(5109..>5535)
                     /locus_tag="VNZ06_14190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW21946.1"
                     /translation="QRAYMSPRNLSVLLVEDSRVLAERLRETLLSVPGVQLAATVDSE
                     AEAVAALQRHPVDVVLLDLHLRQGTGFGVLRAIPGSRAKDVVVIVLTNYDLAEYRRAA
                     AALGAKHFLDKLRDFDRLPSLLLQIGAGPGGVPPPENLN"
BASE COUNT         1089 a         1767 c         1709 g          970 t
ORIGIN      
        1 ccgcgcgagg gtgtgggcca tacgtcgccc gccagcagtt tcagcagctg cgtctttccc
       61 gccccattgg ggccgatcag aacccagcgc tgcccgggac gaatggacag gctgacgtcg
      121 cgaagcacgg gcttgccgtc ccgcgctagc cgcacgttcc tgagatcgac acgaatatac
      181 cgggattgcc ggcgcgcgcg cgggtccgcc atcggacgcg cactgtagca aacgaaaaaa
      241 aaacgctgtc ggtattcggc gcccaccgcg gggggtgttt gtgatgagcg ctagtaccga
      301 cagcgttgct gtccgcccga atgagggctg cagttacaga cgaaaatcgc ctggtgaacg
      361 tgagaatgca accgctatgc cagtgcggtc tgtcatggcg tcgacagaag gcagacacgg
      421 tggcgcaatg gctcgtttac ctaacgatta caataggtta catagattct ggcgaacacc
      481 ccgaaggccg ggccgcctcc attcagcgtc gggatgcagt gacctctggc agcgcaaact
      541 ccgaggcgcc cgcaccatca cggggaacgg ggctcaagtt ggcacgagat gcgggcgatg
      601 cgcgtcttcg cgttcgctgg gccaacagcg catcgaacca ccccatctgc ggtatctgac
      661 tgcagatcgc cttgatgaca atcatgatcg gtatgccaag caccaggccg ggcaatcccc
      721 agatccagga ccagaacaac aggctcacga ataccgcaat cgtgctcatg cgaacactgc
      781 gccccgtaag ccaggtctgc agcaccacgc agatcagcgt ggacaacaac accgtcattc
      841 caccgatgat catgccgttc gccagtgaat cgaactgcac agcggcgaat acggtggaac
      901 cgatggcaat cgcggccggt ccgacgtacg gcacgaagtg cagcaccgcg gcggcgaatc
      961 cccatgcgag cgcgtagtgc acaccggtca gagcaaacgc gccccacacc gccagcccca
     1021 gcagcgtgtt ggtgagcacc agacacacca ggtactgatg cagcgagcgc gtgacctcca
     1081 gcaaaatcag ccgaatgccc tcgcgcgtcc gtacgggaat gaaatgcatg tgagcgattc
     1141 gatttcgcag gctcccaccc gccgccagca tccgatagat cagcaacagt atggctgcta
     1201 tctcgccggt gagcgacaga gactgccggg tggcctgcgc cgcaggcggt gcgctctgat
     1261 gtcggtgctc accgaccgcg tcagcgcgca ccgacacagc aagccgattc gtgggccgca
     1321 gcaagggcct aagcttggac gcagtattaa attgctcttt tgtttctttc cggagacccc
     1381 atgagcgaac ccactgatcg caacgcgaat gacacggtac agggcgaagg cgactacgat
     1441 gccgcgaggc gctatcgcga caaagtcgcc gacttcatca agcgatccga tgttgaagat
     1501 ctggcgcgca agagcgcacc gctgtccgcg cgagaagcgc gcgacgacgc gctggccgat
     1561 gagcgcgcac gcggtcgctc caagggggat attcctgcgg acatcggcgt catgtatccg
     1621 caccagggtt cgtctgcgca cgccgagacg agtaccgacc cgcagcggaa aaaatgatcc
     1681 acgccggaca gatcgaacgc tcgcactcca taggtcattg cagctcgccg gattgtatgg
     1741 ggattgcgtt gttgccagca ctaccgcggc cgtgacgcag cagatcttgg cgcatgctcg
     1801 cgagctcttc ggcatgctcc tcttctatgg cgagaatcga ctcaaataac cgccgggtgg
     1861 ttgtgtcctt ctcgccaatg tattgaatga tttcccgata gctttcgatt gcgatacgct
     1921 ccgcgatcag atcctcttcc accatttccg ccagtgactc gccatcgacg tactcagagt
     1981 ggctgcgatc gagcatcccc tgaggactga aattcggctg cccgcccagc tgcacgatgc
     2041 gctcggccag ctggtcggcg tgctgctgct cctcctgtga atgtttcagg aattcgtctt
     2101 tgatggcttc ggccagcgag ccgctcgcca tgaagtaatg tcggcggtac cgcagcacac
     2161 acaccagctc ggtcgccaat gcctcgttca gcagccgcac taccacttcg cgatcggccg
     2221 catagttgac ggtcaccgcg ccgtcggcga tgtgctgacg cgcgcggcga cgcagctcgg
     2281 cgatctcgct gagaaagggg taaggttgct cccgttcggt gccagtcatg gctttagaca
     2341 tgatcgcgac cctcggtgtt tcagtttgtg acggagaatg acggcttgcg aggtcgcagg
     2401 attcgcgccg ccagatatcg aagaatcatt ggccgcagca gcggcgccag cccggccagc
     2461 cgccacgcgg tcttcgggcg agacatcgcc agtgccagcg ccgcgttcct gagcatgttg
     2521 cgtccgtgct gcccggtcag ggcccgcatg atgcggcttc ggggaaaaac gttcgggtcg
     2581 gcgggccgcg gctgatcgcc tttcagttca tgcgcggctt ggaagatctc gcgccgtgag
     2641 ctccgcaggc gccggcacag gtcatccctg tgctcatcag tcgccattga ggctctgggg
     2701 cgcggacgcg tgcgcccggg tgagaattcc cagtgaggga tccagctgcg ccagctcgcg
     2761 catatcggcc tgccattcgg ccctgagacg ctcgaagggc acgggccgcc ctggctgcac
     2821 ggccccacgg gcataggccg cgccggccag cgccacgatg acgaaggaaa aacacagcat
     2881 gccgaccacc gccaggcggt tgggtccatc ccagctcgca gcgatcgccc aaacgcacgc
     2941 cagcgacagc gccatgaccc cggccgtgac ggtcactgca agaccaagcg cgcgcctgcg
     3001 agactgacgc agtgcatcgc cagcctcctc gcagagcagt tcgccgtaag ctatgaggtg
     3061 ccgaagcacc gccggggcca ggcgttgaag tgcctcgagc acgggtccgc aaccgggttg
     3121 tctagcggcg cgaggctgcc gccacaccga cgccaacgat gacgccgact gtcgcggcca
     3181 atccgatcgc ggtccacggg ctgtcatgga cgtagtcgtc ggttaccgtc gcggccatac
     3241 gcgcccgatc gcgcagggaa ttcgcggtat caccgatcgt ctgacggaca tcgccgatcg
     3301 cacccgatac ccgggcgcgc agccgcgcca cgtccgcatc gccaacgttg gcgactttct
     3361 tcacgaggtc ttcgacatcg gcaaggaagg cacgcaactc gtcgctggcg tagtccctga
     3421 tattcgcacc gcggcgacgg aagtcggccg tagcgtcctt gcctgcccga tgcaagctct
     3481 cggcagtgtc attcagattc gcattcattg attggtctcc tggtttgccg gcccgcgccg
     3541 ctgctcgccg cagcgatggg tggcagctcg attgccgagc ctcaatcgag catggcattg
     3601 tgtattcagc tatattatcg gctccgtgcg gcaagcgttg taggatcagc ctcacagcgg
     3661 aaaacactgc ggaaaacgat tcatgattcg ctccggtcgc atctcgcgag cgctgacact
     3721 gtcagtcgcc tttctcctgc tggtggcgct ggtagtcatc gccgaaatcg gtcagaacgg
     3781 ccagaaagaa atctatcgcc tcatcaatct ctctcaggaa cgacaggtgt tgctcgccga
     3841 gctggtgagc gagctatcgg atgcggaagc gggccagcgt ggctttctgt tgaccaacga
     3901 tcagaactac ctgatgccct atcaaatcgc gcacgatcgc gtcgagccca cactcaacaa
     3961 acttaccgat ctttttcgcg ataacgacaa gctgctgatg agccgcgagc agcgtgacac
     4021 ggtgcggcac ctgcgcaccc tggtcggaac gggcctgggt gagctcgccg cctcgctggc
     4081 gctctacacc acgcagggac ccgagcaggc actcgcgctg gtgcgcaccg atctcggcag
     4141 ccgcaccatg ggcgagatcc gtgccgaagc gctgaagctg cgtaacttcg aacgcgacgg
     4201 agtcaccgcg gcactggctc gcgcggaacg gctgcgcgtc atttcgcgcc tgctgatggg
     4261 cggtgtggtg atactgaatg tcggtctgat ggtcatggcc gcgcagttgg tggcgcgcca
     4321 ggcgcggcgg cgcgccgagc tcactgagca gctcgctacc gaaaatgagg aactggagcg
     4381 gcgggtacgc cgtcgcaccg cggaactgtc cgcgctctcg agccatctgc agcagctctc
     4441 cgaaaaagag aaggcatcct tggcgcgcga gctgcacgat gagctcggcg gactgctgat
     4501 cgcggtcaag atggacgtct cgtggctgca gaagcgctgg ccgaacagtg atccggacat
     4561 tcaggcacgt tggactcgcg tgctgaaggt gctggacgac ggcgtggact tcaagcgccg
     4621 catcgtcgag agcctgcgcc cgacgctgct ggacaacatg ggactgctgc cagcggtgcg
     4681 ctggatcacg caggagacct gcagtcgtgc cggtctgcaa tgcgccgaaa tctatcctga
     4741 acatgagccc gagctgatcg atgatgcggc gatcatggtc ttccggctgg tgcaggagtc
     4801 gttgatcaat atcgtcaagc acgcccgtgc aacgcaggta aaggtcgaga tcgcactgac
     4861 agacagcgag atgacggtca agatcgagga caacggcgtc ggcatcgaca gcgaccgccg
     4921 cgaagcggtc ggatcgcatg gattggcgac catgcggcac cgtgtgcgca gttttggcgg
     4981 tacctttgac atcgatgcgc cgccacaggg tggcacttgc attcaggcac gcattctgct
     5041 cagggacata ttgaagacgc cgcggccgaa tctgctgacg gtcgccgcca cggtcgcttg
     5101 aagaggccct agttcaaatt ctccggtggc gggaccccgc ccggtccggc cccgatctgc
     5161 agcagcagcg acggcaggcg atcgaaatcg cgcagtttgt cgaggaagtg ctttgccccg
     5221 agcgctgcag cggcacgccg gtattctgcc aggtcgtagt tcgtgagcac gatcacgacc
     5281 acgtccttgg ccctgctgcc cggaatcgcg cgcaacaccc cgaaaccggt tccctgccgc
     5341 agatgcagat ccagcagcac gacatccacc gggtggcgct gtaacgcggc aacggcttca
     5401 gcctcgctgt ccacggttgc cgccagctga actccaggca cgctcagcaa cgtttcacgc
     5461 aagcgctcag ccaaaacccg cgaatcttcg accagcagca ccgagaggtt acgggggctc
     5521 atgtaagccc tctgt
//
LOCUS       DATMRM010000467         7314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3983506, whole genome shotgun sequence.
ACCESSION   DATMRM010000467 DATMRM010000000
VERSION     DATMRM010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3983506"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VNZ06_14195"
     CDS             <1..898
                     /locus_tag="VNZ06_14195"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWW21947.1"
                     /translation="GTPALSSVFETDLTGLTRIHRGKVRDIYAVDTEHMLIVSTDRLS
                     AFDVVLPDPIPGKGRVLNEISLFWFARTQQIVPNHLSGRAVDSVVTDRDQQRLLEGRC
                     VIVKRLRALPIEAVVRGYLIGSGWKEYQAGGSVCGIALAPGLQQAARLPEPIFTPATK
                     APAGAHDENISFEATAKLIGAPLAARVRETALALYHHAAEHARARGILIADTKFEFGL
                     DQNERLTLIDEVLTPDSSRFWPADTWRVGISPPSFDKQYVRDYLETLNWNKRAPGPKL
                     PLEIIQRTGDKYREALRRLTAP"
     gene            855..2288
                     /locus_tag="VNZ06_14200"
     CDS             855..2288
                     /locus_tag="VNZ06_14200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWW21948.1"
                     /translation="MTSTAKRCAGLPRRRRIDLQHGQASGESAWDRLQNLPDKLLFGP
                     EAEAPGASGRLRRAARYPYALIRDLLGGKLTLHAMGLVYTSLLAIVPLLAFSFGILKA
                     FHAQGALEPLVHEFFRPMGSAAEDLTARTMDFANKVRGDLVGSVGLVLLIWTLIGTMK
                     KVEDAFNFIWHVDVPRSFARRMGEYLTLLITGPVLLGALIGLSHLAASSVPVKLLSEL
                     PLLTRLKTLSVELAPFVVVSLLLTALYIGIPNTRVRLTAALAGGFSAGILWAAIGRFF
                     TAFVVYSTRLTVVYAGLAILVSALVWTYFNWIILLLGARVSFYTQNPNYLRLGLNELR
                     LSCVDTERLALSIMYVIAERYRAGLPRLSMSALASVLGYPQIAVARLVHTLESAQLLT
                     AAEDATLLPARDITQLPLLDIVTVARTSSSGLIRTSSGIPSVVRQFCSEMDQAWRAHS
                     AGVMLSDLIERERDTQQQPIVVVNQSR"
     gene            complement(2290..3633)
                     /gene="pmbA"
                     /locus_tag="VNZ06_14205"
     CDS             complement(2290..3633)
                     /gene="pmbA"
                     /locus_tag="VNZ06_14205"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF008268.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HWW21949.1"
                     /translation="MPQPIDASSISELKAVVASALTEAKQGGASQAEADASLQRGLSV
                     TVRLGEVETIEFHRDRGLSVTVYFGTAKGSASTADLRPEAVRETVAKASAIAKHTSAD
                     PYAGLADPAELATRIPQLDLYHPWEIEAEQAIDLARECEAAGLACDARLKNSEGGSVS
                     TGSGARVHGNSLGFLEGLTSTSHSVSCTLVAQERDDMQRDYWYSISRHPSALESPKAI
                     GRRAAQRTLRRLGARQLSTRRSPVLFAPELARGLLGSFVAAVRGPAQYRRASFLLNAA
                     GEQVFPSFLNLVERPHLLQGLASSPYDAEGVATRDRDLVRDGVLQGYVLGSYSARKLG
                     LKTTGNAGGIHNILVEARQTGSFDELLRRMGTGFFVTELMGEGVNAVTGDYSRGASGF
                     WVENGAIAYPVHEITIAGNLREMLRSIVAVGTDVDAQGAIRTGSILLEAMTIAGE"
     gene            3715..4026
                     /gene="yjgA"
                     /locus_tag="VNZ06_14210"
     CDS             3715..4026
                     /gene="yjgA"
                     /locus_tag="VNZ06_14210"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HWW21950.1"
                     /translation="MGEDEDNTEDGDVYISRSQRKRRAEALQRLGVRLTTLRPGRLQK
                     LQLPPELLDALMQARQLRSRAALARQRQFIGRLMRGLDPAALERAQAEVADTHDAKMP
                     R"
     gene            4023..4514
                     /gene="purE"
                     /locus_tag="VNZ06_14215"
     CDS             4023..4514
                     /gene="purE"
                     /locus_tag="VNZ06_14215"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017341565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HWW21951.1"
                     /translation="MKALVGIIMGSSSDWETMSAAADTLTRLGVAHETRVVSAHRTPD
                     LLFEYASSAASRGLEVLIAGAGGAAHLPGMTAAKTLLPVLGVPMPSRALNGIDSLLSI
                     VQMPAGVPVATFAIGSPGATNAALFAAALLAPRYPEIAQALRLSREKQTAAVLSKPDP
                     RTP"
     gene            4511..5614
                     /locus_tag="VNZ06_14220"
     CDS             4511..5614
                     /locus_tag="VNZ06_14220"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HWW21952.1"
                     /translation="MTIGIVGAGQLGQMLALAGYPLGLDFLFVDRSADAPAARVAPIL
                     TGELNDPRLLAQLARRSDVVSFDWENVDVTALRKAAGRTRLAPPLRALAVAQDRLSEK
                     KAFVRLSIPTTHFEAVDSRAALEQAVTRIGLPGVLKTRRLGYDGKGQYVLRTVADIQH
                     AWQTLGSAPLIYEELVPFDYEASIIGARSRSGEIAIYPLNRNYHLGGILRLTLAPWPA
                     PRLNRLAAAHLRRVLRAFNYVGVLAIEYFVHRGRLVANEMAPRVHNSGHWTIEGAVTS
                     QFENHIRAITGLPLGDVSARGHSAMINLIGNIPRADRFLREPGLHWHDYGKQPRPGRK
                     LGHCTLVEQTATRRNVRARTLLARLYPQLPLKP"
     gene            5629..6894
                     /locus_tag="VNZ06_14225"
     CDS             5629..6894
                     /locus_tag="VNZ06_14225"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWW21953.1"
                     /translation="MYLEQFRLKELPFRLSPDPEFLYLSKAHARAKAYMESTIWFADG
                     FVVITGEIGCGKTTLIETFLKELQQDVVVAQVNQTQVSSIEFLQSVLVQFGFKPFRMK
                     KAELLATLNDFLVEQYRAGRKLVLVVDEAQNLTSTVLEEVRLLSGIETTKEKVLRIIL
                     AGQPELNEKLDAPDLEQLAQRIRLRFHLPALSSSEMRNYILHRLEVAGSQGREIFTAD
                     CFDLIYRYTGGVPRLVNTLCDTALIAAATENRDVVARDDIMAVIEELQWSEYASRSGT
                     RLMRVTPDDFAAAATGERRTPEAAPPALARILLASGGRTVAERTLAPGRLIIGRTAAN
                     DLQIESRFVSRHHCQIVTTQQSCVIEDLNSTNGIFLHSKRVRYHNLNDGDVITIGQHE
                     LLYIDERSLQRAPSEAPQAVESHPADAES"
     gene            complement(6920..7165)
                     /locus_tag="VNZ06_14230"
     CDS             complement(6920..7165)
                     /locus_tag="VNZ06_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21954.1"
                     /translation="MRIANPLYESLPLLYVLGGGVLLYGSYRLHSGTLSLVLMIVGAC
                     GAIAGVAIWLRRRDFRAANAEYRSLPGRPDSQDERLR"
BASE COUNT         1305 a         2412 c         2294 g         1303 t
ORIGIN      
        1 cggcacgccc gccctatcct cggtgtttga gaccgatctc acaggtctga cgcgcattca
       61 tcgcggcaaa gtgcgcgata tctatgccgt agacaccgag cacatgctga ttgtttcgac
      121 tgaccggctg tcggccttcg atgtcgtgct gccagacccg attcccggca agggccgcgt
      181 cctgaacgag atttcgctgt tctggttcgc gcgcacgcaa cagatcgttc ccaatcattt
      241 gagtggccgg gcggtcgatt ccgtggtgac ggaccgcgac cagcagcggc tgctcgaagg
      301 gcgttgcgtg atcgtgaagc ggctgcgtgc cttgccgatc gaagcggtgg tgcgcggcta
      361 tctgatcggt tcgggctgga aggaatacca ggccggtggc agcgtgtgcg gcattgcctt
      421 ggccccgggt ttgcaacagg ccgctcgcct gcccgagccg attttcacgc cggcgaccaa
      481 ggcaccggcc ggagcgcacg atgagaacat ctcgttcgaa gccaccgcca agctgatcgg
      541 tgcgccgctc gcggcgcgtg tgcgcgagac cgcgctggcg ctctatcacc acgcggccga
      601 gcacgcccgg gcgcgcggca tcctgatcgc ggacaccaag tttgagttcg gtctcgatca
      661 aaacgagcgg ctgaccctga tcgatgaagt gctgacaccc gactcctcgc gcttctggcc
      721 ggccgacacc tggcgtgtcg ggatcagtcc gccctcgttt gacaagcaat acgtgcgcga
      781 ctatctcgag acgctcaact ggaacaagcg cgctccgggg cccaaactgc cactggaaat
      841 catccagcgc accggtgaca agtaccgcga agcgctgcgc aggcttaccg cgccgtagga
      901 gaatcgatct gcagcacgga caagcttcag gcgagagcgc ttgggaccgg ctgcagaacc
      961 tgccggacaa gctgctgttc ggtccagaag ctgaagcgcc cggcgccagc ggccgactgc
     1021 gacgcgcggc acgctatccg tacgcactga tccgtgacct gctcggcggc aagctgacgc
     1081 tgcacgcgat ggggctggtc tacaccagtc tgctggcgat cgtgccactg ctcgccttca
     1141 gcttcgggat tctcaaagcc ttccacgcgc agggcgcgct tgagccactg gtgcacgagt
     1201 tctttcgccc catgggcagc gcggccgagg atctcaccgc tcgcacgatg gactttgcaa
     1261 acaaggtacg cggcgatctg gtcgggtcgg taggcctggt actgctgatc tggacgctga
     1321 tcgggaccat gaagaaggtc gaggacgcct tcaacttcat ctggcacgtt gacgttccgc
     1381 gcagtttcgc gcgccgcatg ggcgagtatc tgaccctgct gatcaccggg cctgtgctgc
     1441 tgggtgcgct gatcggtctg tcgcacctgg ccgcgagcag cgtacccgtg aagctgctgt
     1501 ccgagctgcc gctgctgacg cgtctcaaaa cactcagcgt ggagctggcg cctttcgtgg
     1561 tggtctcgct cctgctgacc gcgctctaca tcggcatccc caatacccgg gttcgcctaa
     1621 ccgccgcact ggcaggaggt ttttctgccg gcatcctgtg ggctgcgatc gggcgcttct
     1681 tcaccgcgtt cgtggtgtat tccacccggc tcaccgtggt ctacgcgggc ctcgccatac
     1741 tggtgtctgc gctggtgtgg acctacttca actggatcat cctgctgctc ggcgcgcgcg
     1801 tgtcgttcta cacgcaaaac ccgaactacc tgcggctggg gctgaatgag ttgcgcctgt
     1861 cgtgcgtcga caccgagcgt ctggcactga gcatcatgta cgtcatcgcc gagcgctatc
     1921 gtgcgggcct cccgcgtctg tccatgtccg ccctggcatc cgtgctgggt tatcctcaga
     1981 tcgcggtcgc acgtctggta cacacgctgg aatccgcgca gctgctcacc gcagcggaag
     2041 acgcgacact gctgcccgca cgcgacatca cacagctacc gttgctcgac atcgtcacgg
     2101 tagcgcgcac ctccagcagc ggactgatcc gtaccagttc cgggatcccg tcggtggtgc
     2161 ggcagttctg cagcgagatg gatcaggcgt ggcgggctca cagcgccggc gtgatgctga
     2221 gtgatctgat cgagcgtgaa cgtgacaccc agcaacagcc gatcgtcgtc gtgaaccaga
     2281 gccgctaacc tactcgcccg cgatcgtcat cgcttccagc agaatcgatc cggtgcgaat
     2341 cgcgccctgg gcatcgacgt cagtgcccac cgcgacgatc gagcgcaaca tctcgcgcag
     2401 attccccgcg atcgtgatct cgtgcaccgg gtaggcaatc gcgccatttt ccacccagaa
     2461 gccgctggcg ccacgcgagt agtcgccggt cacggcattc acgccttcgc ccatgagctc
     2521 agtgacaaaa aaacccgtgc ccatccgccg cagcagctcg tcgaagctgc cggtctggcg
     2581 cgcctctacc agtatgttgt gaatcccgcc ggcattgccg gtggtcttca gacccagttt
     2641 gcgcgccgag tagctgccga gcacatagcc ctgcagcacg ccgtcgcgca ccagatcgcg
     2701 atcgcgcgtg gcaacccctt cggcgtcgta cggactgctc gcgagcccct gcagcaggtg
     2761 cggccgctca accagattca ggaacgatgg aaagacctgc tcgccggccg cattgagcag
     2821 aaacgacgcg cgccgatact gcgccgggcc gcggaccgca gccacgaaag accctaacag
     2881 tccgcgcgcc agctccggcg cgaacaatac cggtgagcgc cgcgtcgaca actgcctcgc
     2941 gcccagtcgg cgcagcgtgc gctgcgcagc acgtcgtccg atggctttgg gagactcaag
     3001 ggcgctcgga tggcgggaaa ttgaatacca gtagtcgcgc tgcatgtcgt cgcgttcctg
     3061 ggcgaccagc gtacaggaca cggaatggct ggtgctggtg agtccctcga ggaagcccaa
     3121 actgttgccg tgtacccgcg cgccgctgcc ggtgctgacg ctaccgcctt cggagttctt
     3181 cagtcgcgcg tcgcaggcca gtcccgccgc ctcgcactcg cgcgccagat cgatcgcctg
     3241 ctccgcctcg atttcccacg gatgatagag atccagctgc gggatgcgcg tggccagctc
     3301 tgccggatcg gccaggccag cgtacgggtc cgcagaagta tgcttggcaa tggccgatgc
     3361 cttggccacg gtctcgcgca cggcctcggg acgcaaatcg gcggtgctgg ccgagccctt
     3421 ggcggtaccg aagtacaccg tcaccgacag cccccggtcg cgatgaaact cgatggtttc
     3481 cacctcgccc aatcgaaccg taacggacag tccgcgttgc agactcgcat ccgcttcggc
     3541 ctggctggct ccgccctgtt tcgcttccgt cagcgcgctg gcaaccactg ctttcagttc
     3601 gctgatgctg cttgcatcga tcggctgtgg catgagtcga cgtccttagg cagcacccga
     3661 ggcgctgtgc catgatctga tcggctgatt gtagcagcgc gaggcgagcc ggctttgggc
     3721 gaggacgagg acaataccga agacggcgat gtgtacatca gccgctcgca gcgcaagcgc
     3781 cgcgcggagg cgctgcagag gctcggcgtg cggctgacca cgctgcggcc gggacggctg
     3841 caaaagctgc aacttccgcc cgaactgctg gacgcgctga tgcaggcccg acagctgcgc
     3901 agccgcgcgg cactggcccg gcagcgccag ttcatcggcc ggctgatgcg tggactggac
     3961 ccggcggcgc tcgagcgggc gcaggccgaa gtcgcagaca cccatgatgc taaaatgccg
     4021 cgatgaaagc gctggtcggc atcatcatgg gatcctcttc cgattgggaa acgatgagcg
     4081 ctgccgccga tacgctgacc cggctcggag tcgcgcacga aacgcgcgtc gtgtcggcgc
     4141 accgcacgcc ggatttgctg ttcgaatacg cctcgagcgc ggcctcacgg ggtctggaag
     4201 tattgatcgc gggagccggc ggcgccgcac acctgcctgg aatgaccgcg gccaaaacat
     4261 tgctcccggt gcttggcgta ccgatgcctt cgcgtgcact caatggcatc gattcattgc
     4321 tgtcgatcgt gcagatgccc gccggagtgc cggtcgcgac cttcgcgatt ggcagccccg
     4381 gggcgaccaa cgcggcgctg ttcgccgccg cgctgctggc gccacgctat ccggagattg
     4441 cgcaggccct gcggctgtcg cgcgagaaac aaacggccgc ggtactgtcc aagcccgatc
     4501 cgcgcactcc atgaccattg ggatcgtggg cgccgggcag ctcggtcaga tgctcgcctt
     4561 ggccggctat ccactcgggc tcgacttcct attcgtcgat cgcagcgccg atgctccggc
     4621 cgctcgcgtc gcccccattc tgaccggcga gctgaacgat ccgaggctac tggcgcaact
     4681 ggcccgtcgc agcgacgtcg tcagcttcga ttgggagaat gtggacgtca cagcactgcg
     4741 caaggcggcc gggcgaaccc ggctggcgcc cccgctgcgg gcgctggcgg ttgcccagga
     4801 tcgcctgagt gaaaagaaag ctttcgttag gctttccatc ccgaccactc actttgaggc
     4861 ggtggacagt cgggccgccc tggaacaggc agttacgcgt atcggcttgc ccggggtact
     4921 gaagacccgt cgcctcgggt atgacggcaa gggccagtac gtgctgcgta ccgtcgcgga
     4981 tatccagcac gcgtggcaga ctctgggcag cgcgccgttg atatacgaag agctggtacc
     5041 gttcgactac gaggcctcga tcatcggtgc acgcagccgc tccggagaga ttgcgatcta
     5101 tccgctcaat cgcaactacc atctgggcgg aattctgcgc ctgacactgg ccccgtggcc
     5161 ggcgccgcgc ctgaatcgtc tggcagccgc tcacctgcgg cgcgtgctgc gggcgttcaa
     5221 ctacgtcgga gtactcgcca ttgagtactt cgtacaccgt ggccgcctcg tggccaacga
     5281 gatggctccc cgtgtccaca actcagggca ctggacgatc gaaggggcgg tgaccagtca
     5341 gttcgaaaat cacatccggg cgatcaccgg actgccgctg ggggatgttt cagcgcgggg
     5401 acactcggcc atgatcaacc tgatcgggaa tattcccagg gcggatcgat tcttacggga
     5461 accggggctg cactggcatg attacggtaa gcaaccccgc ccgggacgca aacttggcca
     5521 ttgcacactg gtggagcaaa cggccacgcg acgtaatgtc agggcccgga cgctcctggc
     5581 aaggctctat ccgcaactac cactcaagcc gtagtatcct gcgcgcccat gtatctggag
     5641 cagttcagac tcaaggagtt accgttccgg ctgagcccgg acccggagtt cctttatctc
     5701 agtaaggccc acgcacgcgc gaaggcctac atggaatcga ccatctggtt tgccgacggc
     5761 ttcgtcgtca tcaccggtga gatcggatgt ggcaagacca cactgatcga gaccttcctg
     5821 aaggagctgc agcaggatgt ggtggtggca caggtcaatc agacccaggt gtcatcgatc
     5881 gaattcctgc agtcggtgct ggtgcagttc ggcttcaagc cgttccggat gaagaaggcg
     5941 gaactgctgg cgacactcaa cgattttctg gtggagcagt accgcgccgg ccgcaagctg
     6001 gtgctcgtag tggatgaggc ccagaacctg acgtccacgg tgctcgaaga agtgcgtttg
     6061 ctgtcgggca tcgagactac gaaggaaaaa gtcctgcgca tcattctggc gggacagccc
     6121 gagctcaacg agaagctcga tgccccggac ctcgaacagc tcgcgcagcg catacgcctg
     6181 cgctttcatc tgccagcgct gtcgagctcg gagatgcgca actacatcct gcaccgtctc
     6241 gaagtggcag gctcgcaggg ccgtgagatt ttcactgctg actgctttga tctgatttac
     6301 cgctataccg gtggcgtacc gcgcctggtc aacacgctgt gcgacacggc actgatcgcg
     6361 gcggccacgg aaaatcgcga tgtcgtagcg cgcgatgaca tcatggcagt gattgaagag
     6421 ctgcagtggt ctgaatacgc ctcgcgcagc ggcacccgtc tgatgcgggt gactcccgac
     6481 gacttcgcag cggcggccac gggcgagcga cgcaccccgg aagccgcgcc accggcgctg
     6541 gcgcgcatcc tgctggcctc cggcggtcgg acggtcgccg agcgcacgct ggcaccgggc
     6601 cggctgatca tcggtcgcac cgcggccaac gatctgcaga tcgagagccg gttcgtgagt
     6661 cgtcaccatt gccagatcgt gactacgcag caaagctgcg tgatcgagga tctgaacagt
     6721 accaacggga tctttctgca ctccaagcga gtgcgctatc acaatctcaa cgacggcgat
     6781 gtgatcacca tcggtcagca cgaactgctc tatatcgacg agcgttcatt gcagcgcgca
     6841 ccgtccgagg caccgcaggc ggttgaatcc cacccggcgg acgccgaaag ctgagctgcg
     6901 atcccgcggc ggcgctgcgc taacgcagtc gctcatcctg cgaatcgggc cggccgggaa
     6961 gcgagcggta ctcggcgttg gccgcgcgaa aatcacgccg gcgcagccag atcgcaaccc
     7021 cggcgatcgc gccacacgcg ccgacgatca tcagaactaa gctcaacgtg cccgaatgca
     7081 ggcgatagct gccgtagagc aacacgccgc cgcccagcac gtatagcagg ggcaggctct
     7141 cgtagagcgg attggcaatt cgcatgtccg cgccctcccg cgcgcgcgaa acgcgcctga
     7201 agccgcttca ggcggtcccg ccgaccgtga gaccgtctac ccgcaacgtc ggctggccga
     7261 cccccacggg cacactctgg ccttccttgc cgcacacacc gatgccatcg tcga
//
LOCUS       DATMRM010000468         3922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_601565, whole genome shotgun sequence.
ACCESSION   DATMRM010000468 DATMRM010000000
VERSION     DATMRM010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601565"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VNZ06_14235"
     CDS             complement(<1..204)
                     /locus_tag="VNZ06_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21955.1"
                     /translation="MSALEDSALSAAQLLESARRTFDIEAAGLAALRARVDDSFVAAC
                     ALCLKTQGRIVTTGLGKTGHVAHK"
     gene            287..541
                     /locus_tag="VNZ06_14240"
     CDS             287..541
                     /locus_tag="VNZ06_14240"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HWW21956.1"
                     /translation="MDPRQVADLIRQAIPDAQIDVRSDDNTHFAAVLVGTAFEGLRPL
                     ARHQLVYRALGERVGREIHALSIEAYTPREWSDRTGAAGG"
     gene            534..1793
                     /gene="murA"
                     /locus_tag="VNZ06_14245"
     CDS             534..1793
                     /gene="murA"
                     /locus_tag="VNZ06_14245"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWW21957.1"
                     /translation="MDKLNIVGGQPLEGEVRISGAKNATLPILAGALLAEGPVTIGNV
                     PHLQDVTTTIELLGRMGVTVTIDERMRIEVDSGTIREYFAPYELVKTMRASILVLGPL
                     LARFGRADVSLPGGCAIGARPVNIHVAGLQAMGADIHIEGGYIRARAARLKGARLVLD
                     TVTVTGTENLMMAAALADGETVIENAAREPEVVDLANFLVAMGAIIAGAGTDRIVVQG
                     VRKLCGVNYDVLPDRIEAGTYLVAGTITRGHVRIKNVNPAHLDAVIVKLREAGASVAA
                     GDSWVEVDMRGRRAKAVDVRTAPYPAFPTDMQAQFAALDTVAAGVGTVVETIFENRFM
                     HMLEMRRMGAEIRLEGNTAIIHGVERLTAAPVMATDLRASASLVLAGLVAEGQTEIER
                     IYHIDRGYETIEEKLAQLGARIKRVPN"
     gene            complement(1801..3069)
                     /locus_tag="VNZ06_14250"
     CDS             complement(1801..3069)
                     /locus_tag="VNZ06_14250"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWW21958.1"
                     /translation="MNSNRVVRALEFFAGTVVGGLALAFVIVYIHPQWLQPRAATAPE
                     TPAALSPAPVPAVHAEPVSVPPDRASALAHESDQLSRDLARLPANASATGSFALAVKL
                     ASPAVVNISTQRLVTEHIQPSAFDQIFGDLQPFYRRRVESALGSGVIIDSAGHIVTNN
                     HVIANADVINVQLADGRVSRATEVGRDPDTDLALLSVKLKDLPVMPLGRSDELQVGDA
                     VLAIGNPLGLSQTVTHGIVSATGRSQLGVATFENFIQTDAAINAGNSGGALVNEHGEL
                     IGINTAVLNKSGGGRISVEGIGFAIPVNLVRGVIRDILDHGRVIRGWIGILPEDVDDE
                     QAHQAGLSRGGVVITNMYRNSPAVDASLRIGDIITAVDGRPVHSAQEALSQIAIKKPG
                     ANLRLHVLRARTEVDVAVHVSERPKLMQGT"
     gene            3176..3820
                     /gene="petA"
                     /locus_tag="VNZ06_14255"
     CDS             3176..3820
                     /gene="petA"
                     /locus_tag="VNZ06_14255"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HWW21959.1"
                     /translation="MSDVGAIADLPDPEIDASRRKFLTVATSATAAVGAVIAAIPFVE
                     SWTPSERARALGAPVEVDLTKLAVGQMVTPTWRRQVIYVVRRDKAMVDSLAKHDANLK
                     DAKSGDSIQPDYAKNEMRSRREDLLVLIGICTHLGCLPKPFFDAGDPVLGPDWPGGFR
                     CPCHGSRFDLAGRVFNGSPASTNLSIPPYSFKGDNTLVVGLDDAAQTTQKEGAA"
BASE COUNT          684 a         1207 c         1304 g          727 t
ORIGIN      
        1 cttgtgggcg acatgaccgg tcttgccgag tccggtcgtg acgatccggc cctgcgtctt
       61 caggcacaga gcgcaggcgg ccacgaagct gtcgtcgacg cgcgcccgca gcgccgccag
      121 gcctgcggcc tcgatatcaa aggtgcgtcg cgcggactcg agcagttgcg ccgcgctcag
      181 ggcgctgtct tccaaagcac tcattaagcc gggcatgata gcgccttcac gccgtgcgca
      241 tcgcgccctg cgcctgcctg cgcaattgcc ttaaagtagc gaccccatgg atccaagaca
      301 ggttgcggac ctcatccgcc aggccattcc ggatgcgcag atcgacgtgc gctcggacga
      361 caacacgcat tttgccgcag tgctggtagg gaccgccttc gagggcttgc gcccgctcgc
      421 gcgccatcag ctggtgtacc gtgcactggg cgagcgcgtc ggccgtgaaa tccacgcctt
      481 gtcgatcgag gcgtacaccc ccagggaatg gtccgatcgc accggggccg ccggtggata
      541 agctcaatat tgtgggtggc cagccgctgg aaggtgaggt ccgcatttcc ggcgccaaga
      601 acgcgacact gccgatcctg gccggggctt tgctggccga agggccggtc accatcggca
      661 atgtgccgca cttgcaggat gtgacgacga ccatcgagct gttgggacgc atgggggtca
      721 cggtcacaat cgatgagcgc atgcgcatcg aggtcgattc ggggacgatt cgtgagtact
      781 tcgcgcccta tgagctggtg aagacgatgc gcgcgtcgat cctggtgctg ggcccgttgc
      841 tggcgcgctt cggtcgtgcg gacgtctcac tgccaggcgg ctgcgcgatt ggcgcgcgac
      901 cggtcaatat ccacgtcgct ggcctgcagg cgatgggcgc cgacattcac atcgagggtg
      961 gctatatccg cgcccgtgcc gcacgcctca aaggcgcgcg cctggtgctg gataccgtca
     1021 cggtgaccgg gaccgagaac ctgatgatgg ccgccgcgct ggcggacgga gagaccgtca
     1081 tcgagaacgc ggcgcgcgag cccgaggtcg tggacctggc caattttctc gttgcgatgg
     1141 gggcgatcat cgccggcgcc ggaaccgacc ggattgtggt ccagggtgta cgcaagctct
     1201 gcggcgtgaa ctatgacgtg ctgccggacc ggatcgaggc gggcacctat ctggtggccg
     1261 ggaccattac ccgcggacac gtgcgcatca agaacgtcaa tcccgcgcat ctggatgcgg
     1321 tgatcgtgaa gctgcgcgag gccggggcct cggtcgctgc cggcgacagt tgggtagagg
     1381 tggatatgcg tggccggcgc gcgaaggcgg tggatgtgcg taccgcgcct tatcccgcgt
     1441 ttcccaccga tatgcaggcg cagttcgcag cgctcgacac cgtcgctgcc ggggtcggta
     1501 ccgtggtcga aaccattttc gaaaaccgct tcatgcacat gctcgaaatg cggcgcatgg
     1561 gcgcggagat tcgtctggaa ggcaataccg cgatcatcca cggggtcgag cgtctcacgg
     1621 cggcgcccgt catggcgacc gatctgcgcg cctcggccag tctggtactg gcgggcctgg
     1681 tggccgaggg ccagaccgag atcgagcgca tttatcacat cgatcgcggc tacgagacga
     1741 ttgaggaaaa gctcgcgcag ctgggcgccc gcatcaagcg ggtgccgaac taagcttgct
     1801 tcaggttccc tgcatcaatt tcggtcgttc gctcacatgt accgcgacgt ccacctcggt
     1861 gcgcgcccgc agcacatgca accgcagatt ggcaccgggt ttcttgatgg cgatttgcga
     1921 cagggcttcc tgtgcactgt gcacggggcg tccgtcgacc gcagtgatga tgtcgccgat
     1981 ccgcaagctg gcatcgaccg ccgggctgtt gcgatacata ttggtgatga cgacgccgcc
     2041 tcgtgacaac ccggcctgat gcgcctgctc gtcatcgaca tcctcaggca gaatcccgat
     2101 ccagccgcgg atcacgcggc catgatccag gatatccctg atcacgccgc gaaccagatt
     2161 caccggtatc gcgaacccga ttccttcgac gctgatgcgg ccgccaccgc tcttgttcag
     2221 taccgcggta ttgatgccga tgagctctcc gtgctcgttg accagcgcgc cgcccgagtt
     2281 gccggcattg atcgcggcgt cggtctgaat gaaattctcg aaggtcgcga ctcccagctg
     2341 gctgcggccg gtagcgctga cgatcccatg ggtgacggtc tgcgacaggc ccaagggatt
     2401 gccgatcgcg agcaccgcat cgccgacctg tagttcatcc gatcggccca gtggcatgac
     2461 gggcaggtct ttcagcttga ccgacagcag cgccagatcc gtgtccggat cacggccgac
     2521 ctcagtagct cgcgacaccc gcccgtccgc aagctgcacg ttgatgacgt cggcgttcgc
     2581 gatgacgtga ttgttggtga cgatgtgccc ggccgaatcg atgatgacgc ccgagcccag
     2641 agcgctctcg acccgacggc ggtaaaacgg ctgtaaatcg ccgaagatct gatcaaatgc
     2701 ggagggctga atatgctcgg tgaccagtcg ctgtgtcgaa atattgacga ccgccggtga
     2761 ggcgagtttg acagcgagcg cgaacgagcc ggtggcgctg gcgttggccg gcagccgggc
     2821 cagatcgcga ctgagctgat cggactcgtg agccagcgca ctggcgcgat cgggcggaac
     2881 cgagaccggt tcggcgtgca ctgccggtac cggtgccggc gacagtgcag ccggggtttc
     2941 tggcgctgtg gcggcgcgtg gttgcaacca ttgcgggtgg atgtagacga tcacgaaggc
     3001 cagcgccaag ccgccgacca ccgtgccggc gaagaattca agcgcacgta ccacgcgatt
     3061 cgagttcatg agtcgtccga gagcctccga ttggttgtgt ataatgcgat agttcccgaa
     3121 cgtttacgta gccttgcgtc atgcgtggcg tctcttttga tagggtgagc gatacatgag
     3181 cgatgtcggt gcgattgccg atctgccgga tccagaaatc gatgcgagcc gccgcaagtt
     3241 cctgacggtg gcgaccagcg ctaccgcggc ggtgggggca gtcatcgcag cgattccgtt
     3301 cgtggagtcc tggacgccgt cggagcgcgc ccgcgcactc ggtgcgccgg tggaggtgga
     3361 cctgacgaag ctggctgtcg gccagatggt cacgccgacc tggcggcgcc aggtcatcta
     3421 cgtcgtgcgg cgcgataagg ccatggtcga ctcgctggcc aagcacgacg ccaacctcaa
     3481 ggatgcgaag tccggagatt ccattcagcc cgactatgcg aagaacgaaa tgcgctcgcg
     3541 ccgtgaggat ttgctggtgt tgattggcat ctgtacccat ctgggctgcc tgcccaagcc
     3601 gttttttgat gccggagatc cggtgctcgg tccggactgg ccgggcggtt tccgctgtcc
     3661 gtgccatggg tcgcgtttcg atttggccgg acgcgtattc aacggctcgc cagcttccac
     3721 caacctttcc attcccccgt acagcttcaa gggcgacaac acattggtag tcgggctcga
     3781 tgatgctgcc cagaccacgc agaaagaagg agcggcctga tggcgtacga ggcaacgagc
     3841 aaggcagcga gcgctcctgg cttctgggga tggctcaaca agcgattgcc gctggattct
     3901 ctgtggcgct cccagtgggg cg
//
LOCUS       DATMRM010000469         3739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3044898, whole genome shotgun sequence.
ACCESSION   DATMRM010000469 DATMRM010000000
VERSION     DATMRM010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3044898"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VNZ06_14260"
     CDS             <1..765
                     /locus_tag="VNZ06_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWW21960.1"
                     /translation="LISLACVLIPMLASREGVTPHYELLSQPPMAAHWLGTDPLGRDM
                     LLRVMWGCRISLAIGLSASLISVLIGVSWGAIAGYAGGMTDSLMMRAVDILYSIPYVP
                     FVIVLTVLFGHQFWLLFVAIGAVSWLDIARIVRGQTLSLKQREFIEAARVSGTQGFGM
                     IRRHIVPNLLGIVMVYATLTIPTVILFEALLSFLGLGVQSPLASLGSLVADGAAEMQT
                     HPHLLIVPAVFLGLIIYCLNYLGDGLRDAFDPRDHG"
     gene            765..1763
                     /locus_tag="VNZ06_14265"
     CDS             765..1763
                     /locus_tag="VNZ06_14265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWW21961.1"
                     /translation="MGDAPLLQIHALSVGYPGPRGPIGALRGVDLALAPGECLAVVGE
                     SGSGKTQLLLSILGVNGANARLGGSIRYRGQELIGLSGAAFNAVRGGRIGIVFQDPMT
                     ALNPYLRVGVQITEVLRQHRGVAQAAAERRALELLELLQVPDAAHRMRQYPHELSGGL
                     RQRVTIAMAMIGEPEILLADEPSTALDVTVQAQVLTLLRELRARTGTAIVLVTHDLGV
                     TAQLADRVAVMYAGRIVEQAPVRELFARPQHPYTEALQGSILRIDSARRTRLPSIAGA
                     PPDPAALPAGCPFAPRCLYAAALCRSVEPALTQTAPGHWKACHHEGALGRLRGDAA"
     gene            1760..2755
                     /locus_tag="VNZ06_14270"
     CDS             1760..2755
                     /locus_tag="VNZ06_14270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HWW21962.1"
                     /translation="MTVPAASLAPLLSVRALAVAYRTLYQGRSVTLPAVNGVSFDLAP
                     GEALGVVGESGSGKSSLARALLRLVKAASGSAQFQGVDLLRVRGAQLRSLRRHLQLVF
                     QDPLASLDPRMTVSDIIAEPLLTFEPRLSAAQRLERVAAMLRVVGLDADHLPRYPHEF
                     SGGQAQRIAIARALIAGPDLIVCDEPLSALDVSIKSQISNLLKDLQRERGLSLLFISH
                     DLAAVRFLCDRVLVLYLGRVLEIAATEALFGEACHPYTRALISAAPVPDPERVPEPLA
                     LLDAEIPSPLSAPCGCAFRTRCPMAIERCAREVPQLRAVATSLVACHRAEEVRGG"
     gene            3046..>3739
                     /locus_tag="VNZ06_14275"
     CDS             3046..>3739
                     /locus_tag="VNZ06_14275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWW21963.1"
                     /translation="MAAATHRAAHQVLEHGRIFSDPLAVAILGVDQDDIVRHARQTPS
                     SRRMRLFINIRTRFAEDSLASAYAHGVRQLVVLGAGLDTYAYRKELPDGLRVFEVDHP
                     ATQAFKRERLAAAHIDLPSALSYVPVDFERDGCGESLSAAGFDPNARTFVTWLGVIPY
                     LTEQAIWSTLNYIARLPGGAEVVFDYSNPPDQLPPERRAEHEQRAAHVAELGEEWLSY
                     FDTETLHERLFAI"
BASE COUNT          569 a         1209 c         1261 g          700 t
ORIGIN      
        1 ctgatcagcc tcgcctgtgt gctgatcccg atgctcgcca gccgggaggg cgtgacaccg
       61 cactacgagc tgctgtcaca gccaccgatg gccgctcact ggctcggaac cgatccgctc
      121 ggtcgcgaca tgctgctgcg agtgatgtgg ggctgccgca tttcgctggc catcgggctc
      181 agtgcgagcc tgatcagcgt gctgatcggc gtgagctggg gagcgatcgc gggttacgcc
      241 ggcggcatga ccgactcgct gatgatgcga gcggtggaca tcctgtattc gatcccgtac
      301 gtgccgttcg tcattgtgct gacggtgctg ttcggacatc agttctggct gctgttcgtt
      361 gcgatcggtg ctgtctcgtg gctggacatc gcgcgcatcg tgcgcgggca gacgctgtcg
      421 ctgaagcagc gtgaattcat cgaggcagcg cgcgtctcag gcacgcaggg attcggcatg
      481 atcaggcgcc acatcgtgcc gaatctgctc ggcatcgtga tggtctatgc gacgcttacg
      541 attccgacgg tgattctgtt cgaagcgctg ctgagctttc tgggactcgg ggtgcagtcg
      601 ccgctggctt cgctcggttc gctggtggcc gatggcgccg ctgaaatgca gacccacccc
      661 catctgctga tcgtgcccgc cgtgttcctg gggttgatca tctactgcct gaactatctg
      721 ggtgatggcc tgcgcgatgc tttcgacccg agggaccacg gatgatgggc gacgcgccgc
      781 tgctgcagat ccacgcgctg tcggtcggct acccgggtcc gcgcggaccg atcggggcgc
      841 tgcgcggcgt ggatctggcg ctggcgccgg gtgaatgcct ggcggtggtg ggtgagtccg
      901 ggtcgggaaa gacgcagttg ctgctgtcga tcctgggagt gaacggcgcg aatgcgcgcc
      961 tgggcggcag catccgctac cgcgggcagg aactgatcgg gctgtctggc gctgcgttca
     1021 atgcggtgcg cggcggtcgc atcgggatcg tgtttcagga tccgatgacg gcactcaatc
     1081 cgtacctgcg ggttggcgtt cagatcaccg aagtgttgcg gcaacatcgt ggtgtcgcac
     1141 aagctgcggc cgaacgacgc gcgctcgagc tgctcgagct gctgcaagtg cccgacgcgg
     1201 cacaccgcat gcggcaatac ccgcacgaac tgtcaggcgg cctgcgccag cgcgtcacga
     1261 tcgccatggc gatgatcggg gaacccgaga tcctgctcgc ggacgagccg agcaccgcgc
     1321 tggatgtgac ggtacaggcg caggtgctca cgctgctgcg cgaactgcgt gcccgcaccg
     1381 gtacggccat cgtgctggtg acacacgatc tgggcgtcac ggcgcagctc gcagatcgtg
     1441 tcgcggtgat gtacgccggt cgtatcgtcg agcaggcacc ggtgcgcgaa ctgttcgcgc
     1501 gcccgcagca tccctacacc gaggcgctgc agggttcgat cctgcgtatc gattcggcgc
     1561 ggcggacacg gctgcccagt attgccggtg caccgcccga cccggccgcg ctgcccgcgg
     1621 gctgcccgtt cgcgccacgc tgtctgtacg ccgcggcgct gtgtcgcagc gtcgagcccg
     1681 cgttgacgca gaccgcgccc gggcactgga aggcctgtca ccacgagggc gcactcggaa
     1741 gactgcgcgg agatgccgca tgactgtgcc ggcggcatcg ctcgcgccgc tgctgtcggt
     1801 acgagcgctc gcggtcgctt atcgaacgct ctatcaggga cggtccgtga ccttgcccgc
     1861 ggtcaacggt gtgagtttcg atctcgcgcc cggtgaggca ctgggcgtgg tcggggaatc
     1921 gggcagcggc aaatcgagcc tcgcgcgcgc gctgcttcgc ctggtgaagg ctgcgtccgg
     1981 cagcgcgcag tttcagggtg tggatctgct gcgcgtgcgg ggtgcgcagc ttcgctcgct
     2041 acggcgccat ctgcagctcg tgttccagga cccgctcgcc tcgctcgatc cacgcatgac
     2101 ggtcagcgac atcatcgccg agcccctgct cacgttcgaa ccacgactct cggccgcgca
     2161 gcgcctggaa cgcgtcgctg cgatgctgcg cgtggtcggt ctggatgccg atcatctgcc
     2221 acgctatccg cacgagttct cgggcggcca ggcccagcgc atcgcgatcg ctcgcgcgct
     2281 gatcgccggc ccggacctga tcgtgtgcga tgagccgctg tctgcgctcg atgtatcgat
     2341 caagtcgcag atcagcaatc tgctgaaaga cctgcagcgc gaacgagggc tgtccttgct
     2401 gttcatttcg catgatctgg cagcggtgcg attcctgtgc gatcgcgtgc tggtgctcta
     2461 tctgggccgt gtgctggaga tcgccgccac cgaagcattg ttcggtgaag cctgtcatcc
     2521 gtatacgcgc gcgttgatca gcgccgcccc cgtgccagat ccggagcgag tgcctgaacc
     2581 gctggcactg ctcgacgccg agattccctc accgctgtcc gcaccctgcg gttgcgcgtt
     2641 tcggacacgc tgtccaatgg cgatcgagcg ctgcgcgcgc gaggtgccgc agttgcgggc
     2701 ggtggccaca agcctcgtgg cctgccaccg cgctgaggaa gtgcgcggcg gataaatacg
     2761 tccccatatc gcgacatgag agcggcttcg cgatgacatt tgtcggccac ttgaagctga
     2821 ccctccaacc gcgctggccc gacgacctcc ttcagcctcc ccaatctaac gccgggagtc
     2881 tggtatttcg cgatgatggc ctacacctcg gctggcaacc agagcgatct cagcagcacc
     2941 gtgtcggcga tgattcagta ggccgggcga actcaggtgt tcgccgccgc gccgccgtgg
     3001 taagttggca gctatatgga taccaagggt actagccgga ctgcgatggc tgccgcgaca
     3061 caccgagcgg ctcaccaagt actcgagcat ggacgcatct tcagcgatcc ccttgcggtc
     3121 gcaatcctcg gggtggatca ggacgacatc gttcgtcacg cgcgccagac gccttccagc
     3181 cggcgcatgc ggttgttcat caacatccgg acccgtttcg ccgaggactc gctggctagc
     3241 gcctacgcgc acggcgtgcg ccagctagtg gtgctgggcg ccggactgga cacttacgcc
     3301 tatcgcaagg aactgcccga tggcctgagg gtattcgaag tcgatcaccc ggcgacgcag
     3361 gccttcaaac gcgagcgcct ggcggccgca cacatcgatc tgccgagcgc cctgagctac
     3421 gtgccggtcg atttcgagcg cgatggctgc ggcgagagtc tgtccgcggc cggtttcgac
     3481 cccaacgcgc ggactttcgt gacctggctg ggcgtgatcc cgtatctgac tgagcaggcc
     3541 atctggtcca cgctcaacta catcgcccgg ctgcccggcg gagccgaggt ggtgttcgac
     3601 tacagcaatc cgcccgatca gctgccgccc gagcggcgtg cggagcatga gcagcgggcg
     3661 gcgcacgtcg ccgagctcgg agaggagtgg ctgagctact tcgacaccga gacgctgcac
     3721 gagcggctgt tcgcgatcg
//
LOCUS       DATMRM010000470        15095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3984913, whole genome shotgun sequence.
ACCESSION   DATMRM010000470 DATMRM010000000
VERSION     DATMRM010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15095
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3984913"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VNZ06_14280"
     CDS             <1..411
                     /locus_tag="VNZ06_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21964.1"
                     /translation="AEDKEAGSSQFEQRTRELWLQSADALPGSVRSRLTQARYAALAA
                     HSSKSTLSFTRRWMPAGAVAGVVLALFVVLAPHGSSNSVARLALVGGPPVEDLDMLTD
                     SDALPLNGDQDVDFDFYEWAAEEATVASAPAVGS"
     gene            408..716
                     /locus_tag="VNZ06_14285"
     CDS             408..716
                     /locus_tag="VNZ06_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21965.1"
                     /translation="MRFRGGALTIGFGWLALMAAAEAADKPDPALLEFLGSVDSEDKD
                     WHDYLAHTDIEQVAKRAVAARGNPAPASSPAPVTASSPPKTPPVSPTPPANPAPVNQP
                     "
     gene            713..1252
                     /locus_tag="VNZ06_14290"
     CDS             713..1252
                     /locus_tag="VNZ06_14290"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HWW21966.1"
                     /translation="MIRRQVWWLALLLTAAASAWSQEPAPAAGPPPVNWSSLSEQQQR
                     LLENFHGQWDSLPPARQQALSNGAQRWSQMTPEQRNGAQNRFHNWQHLPPAQQSLIRQ
                     RWQRFQQLAPEQQAAIRQNYHAFSGLPPAQRLQLRQRWLNATPQQRLQMLQNQRDRRL
                     RGPAPFRTPHFPPPPEEHR"
     gene            complement(1287..2684)
                     /locus_tag="VNZ06_14295"
     CDS             complement(1287..2684)
                     /locus_tag="VNZ06_14295"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HWW21967.1"
                     /translation="MDLGSVHIGADALIQTSYLITAFLFIMGLKRMSSPVTARSGILW
                     AGAGMVVATAATFFFPGMGNFALMGAAIFVGGIIAWVSGRRVAMTNMPQMIALYNGMG
                     GGGAAAIAAVELYSGRQITSTEATLAVVGGIIGAVSFSGSLIAFAKLQGLIKGSLRFG
                     GQQILNLLILAAALVLGAVIASGYNVTTPVVSALFGAALVLGVSMTLPIGGADMPVVI
                     SLYNALTGLAVAFEGFVLGNAAMIIAGTVVGSAGTLLTQLMARAMNRSLGNVLFSGFG
                     DTGGTSSGTAITGSMKPIEASDAGVMMAFAQKVIIVPGYGMAVAQAQHKVWELTQLLI
                     DRSVTVKFAIHPVAGRMPGHMNVLLAEAGVPYDLIADLDEINAEFDGADVALIIGAND
                     VVNPVARNDKSSPIYGMPILNADKAKNVIVIKRGQGAGFSGIENALFYLDNTRMLYGD
                     GQSAVASLIAAVKSV"
     gene            complement(2684..2992)
                     /locus_tag="VNZ06_14300"
     CDS             complement(2684..2992)
                     /locus_tag="VNZ06_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HWW21968.1"
                     /translation="MTGIVALYIFMLAAFTGYEVIAKVPVILHTPLMSGSNFVHGIVL
                     VGAMVALGAATTPLEKTIGFIGVLLAAGNAVGGYAVTERMLEMFKSSGAKGGGAKGHH
                     "
     gene            complement(2989..4119)
                     /locus_tag="VNZ06_14305"
     CDS             complement(2989..4119)
                     /locus_tag="VNZ06_14305"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HWW21969.1"
                     /translation="MPVTIGALRERVPGETRVALVPEVADKFAAAGARVLIERGAGVS
                     AQFPDALYKKVEWVDDAQKILSSADILLSVQPLSVAQIQSLKAGAVVAGFMQAHAQTD
                     AVKALRDRQITAFAMELVPRISRAQSMDALSSQAAIAGYKAVLIAANTLDKFLPMLTT
                     AAGTIRPAQVLIIGVGVAGLQAIATAKRLGAVVEAYDVRSATKEQVKSLGAKFVETGV
                     SAEGSGGYARELTDEEKKKQQEVLDSRIAVADAVVTTASVPGRRAPRIISKAAVERMK
                     AGAVVVDIAAEQGGNCELTQAGQTVIHQGVKIVGPVNLPAQLALNASEMYARNLYNFL
                     KPAIDKQANLAIDWQDEVFAQSCLTHAGAIKHEPTAKLVEGT"
     gene            4253..4900
                     /locus_tag="VNZ06_14310"
     CDS             4253..4900
                     /locus_tag="VNZ06_14310"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWW21970.1"
                     /translation="MTVDPDQIFLVPHSATHGLSQMVLRTDVAGMPLEWIDYKEAARL
                     YHQDQIAYSCGSLIYRLYGGVNALSGLRTVLEVNSIVATLGHTGNPGNTRHDYVPPLN
                     NETLFRRDAYLCMYCAGRFPSRELSRDHVRPFSQGGLDVWTNVVTACRHCNNEKAWRT
                     PEQAKMQLIAVPFTPTYAEYIFLKGRRVLADQMEYLLAHFPRTSPLHDRIARWLS"
     gene            4932..5822
                     /locus_tag="VNZ06_14315"
     CDS             4932..5822
                     /locus_tag="VNZ06_14315"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HWW21971.1"
                     /translation="MVYLIDASFYVFRAWHSMPPDLIDGQGNPTHALYGFARFLGDLL
                     ERRRPNYVAVAFDESHGKSFRHKLYPAYKANREAAPLELRRQFTLCREFCRHLGIAEF
                     ASPDYEADDLIGTLVHRCRAQGLRVTLVSRDKDLAQLIGPGDVFWDYSDSAEYRYHDI
                     AERFGALPERMADYLALMGDSVDNVPGVPGVGAKTAGALMKLFASLDELFERVDVVAD
                     LPLRGAASLPQRLLEHRGTAYQARALTQIFCNVPLNAARTDLLCRAPDLRRIEAFCDF
                     HGFGSLVRRQAQRLAAVRAA"
     gene            complement(5840..6175)
                     /locus_tag="VNZ06_14320"
     CDS             complement(5840..6175)
                     /locus_tag="VNZ06_14320"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HWW21972.1"
                     /translation="MSEPNSMSRRDALKGFVLLCGAASALGPNREAQAVELPHLSAED
                     PTAKALGYREDAKSVDKKKFPTYAAGQVCSTCMQFQGKPGDAYSGCNIFSGKAVNANG
                     WCQVWVKKA"
     gene            6322..8523
                     /gene="acs"
                     /locus_tag="VNZ06_14325"
     CDS             6322..8523
                     /gene="acs"
                     /locus_tag="VNZ06_14325"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HWW21973.1"
                     /translation="MAKNSEKAKHIESVLNENRSFPPSAEFIARARIKASELAQLHRR
                     AQEDYTRFWGDLARRSLHWHKPFSITLDDSKAPNYRWFTDGELNVSWNCLDAHLGERG
                     HKTAIIFEGEPGDSRRLSYRELHSDVCRFANALRKLGVGHGDRVVIYMPMVPEAVIAM
                     QACARIGAVHSVVFGGFSAIALRDRIRDAEAKLAITADGGWRGGHALNLKGAVDAALA
                     DGCPTIRSVVVLKRTHTQITMHPGRDVWWHDLVDGESPQCEPEWVNAEHPLYLLYTSG
                     STGKPKGIQHSSAGYLLNTRLTCEWVFDLREDDVFWCTADVGWVTGHSYVAYGPLAAG
                     ATIVMYEGAPMIPDGGRFWKICAAHGVTVFYTAPTAIRSLMKLGDEIPSRYDLSKIRL
                     LGSVGEPINPEAWMWYHRVIGRGRCPIVDTWWQTETGAIMISPLPGVTVAKPGSCTQA
                     LPGIFADIVDDRGESVKQPGAGGYLVIRKPWPSLARTIWKDNDRYLSTYWEKFNNRFY
                     LAGDGAHRDSEGYFWIVGRIDDVLNVAGHRLGTMEIESALVAHPRIAEAAVVGRPHEV
                     KGESVFAFVVCRGARPDADLGKLTEELRSWVAQQLSPIAKPDDVRFADALPKTRSGKI
                     MRRLLRAIARGEEITQDVSTLENPAIVAQLRGENMAAHAASIRSSTPAPHKPKARNTT
                     KSKVKAKAKAKTKVTAKAGRKPVRRLTARHRPPSKAKAKTKVSRRKVRARR"
     gene            8520..9233
                     /locus_tag="VNZ06_14330"
     CDS             8520..9233
                     /locus_tag="VNZ06_14330"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWW21974.1"
                     /translation="MKWRVVSAAALVSAGLCLSACRTPPGPKTTPGALVQPAAPPGAL
                     VPGTHYTVDAQRSQVLVLVYRDGAMAHLGHNHVISVRQLSGSVSMGSDPGHSSFQIDF
                     PVDAMSVDEAALRAAEGADFQSTVDEAAIAGTRDHMLGEALLNSKQFPQIHLASEQIH
                     DSGGSLSALTTIVVRNTTAHVQIPVTLQTSGDDLTVNGEFDLTHTQLGLTAYSVALGA
                     LRVAERIHIRYRLIAHRQP"
     gene            complement(9279..9998)
                     /locus_tag="VNZ06_14335"
     CDS             complement(9279..9998)
                     /locus_tag="VNZ06_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21975.1"
                     /translation="MFGRFLEIGLATRDITASFTFYDRLGFGQLMTSDAWPHRYGVLS
                     DERLYVGLHEREMTSPSVTFVLPDLVHALPRLRSQRLEPDLALLGEDRLNQVVLRDPA
                     GHAVTLLEARTYSPAPQGSAKQSLCGYFSHLSLPQTDFDSARIFWELAGFVALAEEDL
                     PFPHLPLTSDHLDLAFHQRRTFDAPLLVFECVDIAAAMQRMRELELPTSTHLPRGLDR
                     KRSCLIESPEGTALLIVPVSD"
     gene            complement(9998..11035)
                     /gene="hemH"
                     /locus_tag="VNZ06_14340"
     CDS             complement(9998..11035)
                     /gene="hemH"
                     /locus_tag="VNZ06_14340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HWW21976.1"
                     /translation="MNDSSLNPGGVSPAVLLVNTGTPEAASTAAVRRFLRRFLSDPRV
                     IELPRVLWLPLLYGLVLPVRSPRSAHKYQRIWRDEGSPLLVYTGQLREALEREIHALR
                     GHVQVEQAFLYSAPFVGQTIRRLRDAGVRRLLVLPLFPQSSGSTTGAVYDQVAQALRD
                     WRALPQLRYIANYHAEPDYIRALAASLTDYRRLHGKGGHLLMSFHGIPLSYVTAGDRY
                     ADECRQTAALLAAAMDLEQRDWSLTFQSRFGANRWLTPATDEVLAQLPRRGVRAVRVV
                     CPGFASDCLETLEEIALVGREIFLKAGGEQFDYVPALNARSDHAAMLARLVARSIADW
                     SAPSGMRAPAS"
     gene            complement(11054..11722)
                     /locus_tag="VNZ06_14345"
     CDS             complement(11054..11722)
                     /locus_tag="VNZ06_14345"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWW21977.1"
                     /translation="MNQFQSLMARDGHTFSAYLASACDRARGAVVVVQEIFGLTAHIR
                     RVADHFAHEGYLAIAPALFDRIHRNVVLGYTPEDAELAMGYRLQVPLAKALLDIAAAA
                     AVVRHAGRVATVGFGWGGTLTWAAAGSVPMAAAVCYDGAKMLEHSPKTPPCPTQFHFG
                     EHDSGHAASEVPQLREAYPAGEYYLYPAAPDFANQDRPDRYRAEDAALARSRTLAFLA
                     QRVG"
     gene            complement(11719..13011)
                     /locus_tag="VNZ06_14350"
     CDS             complement(11719..13011)
                     /locus_tag="VNZ06_14350"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HWW21978.1"
                     /translation="MQVGSTTRRVAIIGGIRTPFARSNTAYASSDDQELLIASLRGIV
                     DHFNLHGERLGDVIAGAVIKHPKDYNLTRESLLSSGLDPQTPGLDVQRACGTSLEAAI
                     LVANKIALGQIEAGIAAGVDTTSDPPIAYPRSFQQKLLQSARGRSFGQRMRPWLSLRP
                     RDFKPVLPAVVEPRTGLSMGQSCELMVKTWNISREDQDRIAYDSHVKAATAWREGFYE
                     DLVIPFRDLNTDNNVRTDTSVERLGKLKAVYDFSGQGSLTAGNSTPLTDGSAALLLAS
                     EQWAARRNLPILAYITAGKSWAVDFASGKEGLLMAPAYAVAEILKDTGLRLQDFDFYE
                     IHEAFAAVPLCLFKAWESADYCRDRLGLSEALGSIDYAKLNLKGGSVAIGHPFAATGA
                     RILATLAKQLSLAPQSKRGIASACTAGGMGVTAILERA"
     gene            13116..14687
                     /gene="gshA"
                     /locus_tag="VNZ06_14355"
     CDS             13116..14687
                     /gene="gshA"
                     /locus_tag="VNZ06_14355"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HWW21979.1"
                     /translation="MLDRVYERRLAGLINSREQRLLCEGPRGLERESLRVHPDGRIAQ
                     TPHPVGLGSALTNPHVTTDYAEALTELVTPTFQSNAALLQYLQELHQFVYRRIADELL
                     WATSMPCALRGDADVPIARYGPSHQGRLKSIYRHGLLIRYGGIMQAISGVHFNYSFPQ
                     QFWPLYAQVYQSHDEGQDFVSARYFDMLRNYRRHGWIVSYLFGVSPALCRSFLQNRQD
                     HQLEPLGDDTFIGPHATSLRMSDIGYRNRGQSEVAVSVNHIDEYLRDLRRAVTTPHPP
                     FLKLGVKVDGEYRQLSGNLLQIENEYYSYVRPKRALRAGERTTHALARAGVEYVEVRA
                     LDNSAFDPVGVNLRKLYFLEAFVQLLMFKSSAPIDASEEESIDRNHLLVARHGREPGL
                     TLVRDGRNVPMIGWAEELLDSMTGICELLDSGHPERPYSATLKDQQAKLMDVEQTPSA
                     RLLRELREHGESFSALALRYSTEHRRSIDAQPTNDRRSQEFAAEAEQSLEAQRSLEFS
                     QQGSFDEYLARYLAD"
     gene            14856..>15095
                     /locus_tag="VNZ06_14360"
     CDS             14856..>15095
                     /locus_tag="VNZ06_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWW21980.1"
                     /translation="MKLVTAIIKPFKLDDVRAALSELGVSGMTVTEVKGFGRQRGHTE
                     LYRGAEYVVDFVPKTRVEVAVRAELVDQVIEAVLKA"
BASE COUNT         2785 a         4768 c         4785 g         2757 t
ORIGIN      
        1 gccgaggata aggaagcagg aagcagccag ttcgagcagc ggacccgcga gctatggctg
       61 caaagcgcgg atgcgctgcc gggttcggtg cgttcgcgtc tgacgcaggc tcgctacgcc
      121 gcactcgcgg ctcactcttc gaaaagcaca ctgtcgttta cccgtcgctg gatgccggcg
      181 ggcgccgtgg ccggtgtcgt actggcattg ttcgtggtgc tggcgcctca cggcagcagc
      241 aacagcgtgg cacgactggc gctggtcggc ggccccccgg tggaggacct cgacatgctg
      301 accgactcgg acgcactgcc gctcaatggc gatcaggacg tggatttcga tttttatgag
      361 tgggctgccg aggaagcaac cgtcgcctcg gcgcctgcag ttggatcgtg aggtttcgag
      421 gcggcgcact gactatcggc ttcggttggc tggcactgat ggcggccgcg gaggctgctg
      481 acaaacccga ccccgcgctg ctcgaattcc tcggatcagt ggatagcgaa gacaaggatt
      541 ggcatgacta tctggcgcat accgacatcg agcaggtcgc caagcgtgcg gtggccgcgc
      601 gaggcaaccc tgcgccagcg tcatcgccgg ccccggtcac cgcatcgagt cctccgaaga
      661 cgccgccggt cagtcctacg cctcccgcca atcctgcgcc ggtgaatcaa ccatgattcg
      721 caggcaggta tggtggctgg cactgctgct caccgcagcg gcttcagcct ggtctcagga
      781 gcccgcgccg gctgccggcc cgccgccggt caactggtcg tcactgtcgg agcagcagca
      841 gaggctgctg gagaatttcc acggccaatg ggacagtttg ccgccggcgc gccagcaggc
      901 cctcagcaac ggcgcgcagc gctggtccca aatgacgccc gaacagcgca acggtgcgca
      961 aaaccgtttt cacaactggc agcaccttcc tccggcgcag caatcgctga tccgtcagcg
     1021 ctggcagcgg ttccagcagt tggcgcctga acagcaggct gccattcgcc agaactatca
     1081 cgccttttca ggtttgccgc cggcgcagcg tctgcagctg cgccagcggt ggctgaatgc
     1141 gacgcctcag cagcgtctgc agatgttgca gaaccagcgc gaccgccgcc tgcggggccc
     1201 ggcgccgttt cgcacaccgc atttcccacc gccgcccgaa gagcatcgtt agtcgtagta
     1261 cgcgatggcg cgccgaacgg atggggtcag accgacttca ccgccgcgat cagtgacgcc
     1321 acggcggatt ggccatcgcc atacagcatg cgtgtgttat cgaggtagaa cagcgcgttc
     1381 tcgataccgg agaatccggc accctgtccg cgcttgatga cgatgacgtt cttcgccttg
     1441 tcggcgttga gaatcggcat gccgtagatc gggctcgact tgtcgttgcg cgcgaccgga
     1501 ttgacgacgt cgttggcgcc gatgatcagc gcgacatccg caccgtcgaa ctcggcattg
     1561 atctcatcca ggtcggcgat caggtcatac ggtacgccgg cctccgcgag caacacgttc
     1621 atgtggccgg gcatgcggcc ggcgacggga tgaatggcaa acttcaccgt cacgctgcga
     1681 tcgatcagca gctgcgtgag ctcccagacc ttgtgctgtg cctgcgcgac cgccatgccg
     1741 tatccgggga cgatgatgac cttctgggca aaggccatca tgacgccggc atcgctggct
     1801 tcgatcggct tcatgctgcc ggtgattgca gtgccggagc tcgtcccccc ggtatcgcca
     1861 aaaccggaaa acagcacatt gccgagcgag cgattcatgg cccgcgccat caactgcgtg
     1921 agtagcgtgc cggccgaacc gaccaccgtg ccggcgatga tcatcgcagc gttgccgagc
     1981 acgaaccctt cgaaagcgac cgccaggccg gtaagggcgt tgtacaggga gatcaccacc
     2041 ggcatgtcgg caccgccgat cggcaacgtc atcgagacgc ccaataccag cgccgcaccg
     2101 aacagcgcac tgacaaccgg cgtggtgacg ttgtagcccg aggcgatcac tgcgccgagt
     2161 accagtgctg ccgccaggat cagcaggtta agaatctgct ggccgccgaa gcgcagcgag
     2221 cccttgatca gtccctgcaa cttggcgaat gcgatcagac tgccgctgaa cgacacggcg
     2281 ccgatgatgc cgccaaccac cgccagcgtg gcctcggtgc tggtgatctg ccggccgctg
     2341 tagagctcga ccgcggcgat ggccgccgcg ccgccaccac ccatgccgtt gtacagtgcg
     2401 atcatctgcg gcatgttggt catcgcgaca cggcgcccgc tgacccaggc gatgatgccg
     2461 ccgacgaaga tcgcggctcc catcagggca aaattgccca tgccggggaa gaagaacgtg
     2521 gccgcggtgg ccacgaccat gcccgcgccg gcccacagga tgccgctgcg cgccgtgacc
     2581 ggcgagctca tgcgcttcag tcccatgatg aacaggaacg ccgtgatcag gtagctcgtc
     2641 tgtatcagcg cgtccgcgcc gatgtgtacg cttccgaggt ccatcagtga tgccccttgg
     2701 ctccgccacc cttcgcgccg ctggatttga acatctcgag catgcgttca gtgacggcgt
     2761 agccgcccac ggcgttgcca gccgcgagca gcacgccgat gaacccgatc gttttctcga
     2821 gcggcgtggt cgcggcgccg agcgccacca tggcgccgac cagcacgatg ccgtgaacga
     2881 agttcgatcc cgacatcagc ggggtgtgca gaatgaccgg caccttggcg atcacttcat
     2941 agccggtgaa cgcggccagc atgaaaatat agagcgcgac gattccggtc atgtgccctc
     3001 caccagctta gcggtaggtt cgtgtttgat ggctcccgcg tgggtcaggc atgactgggc
     3061 gaacacctcg tcctgccagt cgattgcgag gttggcctgt ttgtcgatgg ccggctttaa
     3121 aaaattgtaa aggttgcggg cgtacatttc gctcgcattc agcgcgagtt gcgctggcag
     3181 attgacgggt ccgacgattt tgacgccttg atgtatcacg gtctgccctg cctgggtaag
     3241 ttcgcagttg ccaccttgtt cggcggcaat gtccaccacc actgccccgg ctttcatgcg
     3301 ctccaccgcc gctttggaga tgatgcgcgg cgcacgtcgt cccggtaccg atgccgtcgt
     3361 gacgaccgca tcggcgaccg cgatacgaga gtcgagtact tcctgctgct ttttcttttc
     3421 ctcgtcggtg agctcgcggg cgtaaccgcc gctgccttcc gccgatacgc cggtttcgac
     3481 gaatttcgcg ccgagcgact tcacctgctc cttggtggcc gagcgcacgt cgtacgcttc
     3541 gacgaccgct ccgaggcgtt tggcggttgc aatagcctgc aagcctgcca cgccgacgcc
     3601 gatgatcagc acctgagccg gccggatggt acccgcggcg gtggtgagca tcggcaggaa
     3661 cttgtcgaga gtgttggcgg cgatcagcac ggccttgtag cccgcgatcg cggcctggga
     3721 ggacagcgcg tccatcgatt gcgcgcgcga gatacgcggt accagctcca tcgcaaaggc
     3781 ggtgatctgc cgatcgcgca gggctttgac cgcatcggtt tgggcgtggg cctgcataaa
     3841 gcctgcgacc accgctccag ctttcaggct ctggatctgc gccacgctca gaggctgaac
     3901 cgacagcagg atatcggcgc tggaaaggat cttctgggcg tcgtcaaccc attcgacttt
     3961 cttgtacaaa gcatcgggaa actgcgcgct gacgccggct ccgcgctcga tcagtacccg
     4021 ggcgccggcg gcggcaaact tgtcggcgac ttccggaacc agggcgacgc gtgtttcccc
     4081 tggcacgcgc tcgcgcagcg cgccaatcgt aactggcata gtgttctccc taccttgagg
     4141 cgcgaccgca tccgaaggca cgtgcacgtt tggagtgtgc acgcgttcaa atccttatgc
     4201 gttgaattct cgcataatcc tgccgtatct tacagtttat gagggttaag tggtgacggt
     4261 cgatccggat cagatatttc tggtaccgca ttctgcgacg catggactgt cgcagatggt
     4321 gctgcggacc gatgttgccg gaatgccgct ggaatggatc gactacaagg aagcggcccg
     4381 tctctaccac caggaccaga tcgcctacag ctgcggatca ctgatctacc ggctctacgg
     4441 aggcgtcaac gccctcagcg ggctgcgtac ggtgctggaa gtcaattcga tcgtggcgac
     4501 gctgggtcat accggcaatc caggcaatac ccggcacgac tatgtacctc ccctcaacaa
     4561 cgaaaccctg ttccgtcgcg acgcctacct gtgcatgtat tgcgctgggc gttttccgag
     4621 ccgggaattg tcgcgtgatc acgtacgccc gttctcgcag ggcggcctgg acgtgtggac
     4681 caacgttgtc acggcctgcc gtcactgcaa caacgagaag gcctggcgta ccccggaaca
     4741 ggccaagatg cagttgatcg ccgtaccgtt cacgcccacc tacgcggagt acatcttcct
     4801 caagggcagg cgggtgctgg cggatcagat ggaatacctg ctggcgcact ttccacgcac
     4861 cagcccgttg cacgaccgga ttgcgcgctg gctcagttag cgcgcgcgtt gggttagact
     4921 gaccgcccat agtggtctac ctgatcgacg catcgttcta cgtctttcgc gcctggcact
     4981 cgatgccgcc cgatctcatc gacgggcaag gcaatcccac acacgccttg tacgggttcg
     5041 cgcgctttct gggcgatctg ctggagcgca gacgtccgaa ctacgtcgcg gtggctttcg
     5101 atgagagcca cggcaagtcg tttcggcaca agctctatcc ggcgtacaag gccaatcgcg
     5161 aggccgctcc gctcgagctg cggcgccagt tcactctgtg ccgcgagttt tgccggcact
     5221 tggggatcgc cgagttcgcc agccccgact acgaagccga cgatctgatc ggcacactgg
     5281 tacaccggtg ccgggcccag gggctgcgcg tcacgctggt ttcgcgcgac aaggaccttg
     5341 cacaactgat cggccccggt gatgtgttct gggactacag cgattcggcc gaataccgct
     5401 accacgacat cgccgagcgt ttcggcgcgc tgccggaacg gatggccgat tatctggcgc
     5461 tgatggggga cagtgtcgat aacgtgcccg gggtacccgg ggtgggagcg aagacggccg
     5521 gagcgctgat gaagctgttc gcgtcgctgg acgagctgtt cgagcgcgta gacgtggtcg
     5581 ccgacctgcc gctgcggggt gcggcctcat tgccgcaacg actgctcgag catcgcggta
     5641 ccgcttatca ggcgcgggcg ttgactcaga ttttctgcaa cgtgccgctc aatgcggcgc
     5701 gcacggacct cttatgccgt gccccggatc tgcggcgcat cgaagcattt tgcgactttc
     5761 acggcttcgg cagcctggtg cgccgccagg cgcagcgcct ggcggcggta cgggccgcct
     5821 gaagatcagg ccggtcgctt caggccttct ttacccagac ctgacaccat ccgttcgcgt
     5881 tcaccgcctt gcctgagaag atgttacagc ccgagtaggc atctccaggt ttgccctgaa
     5941 actgcatgca ggtgctgcac acctggccgg cggcataggt cgggaacttc ttcttgtcca
     6001 cgctcttcgc gtcctcgcgg tagcccaggg ccttggccgt cggatcctcg gccgacaggt
     6061 gcggcagctc cacggcttgc gcttcgcgat tgggccctaa ggcactggcg gcaccacaga
     6121 gcagtacaaa gcccttcagt gcatcgcgac gtgacatcga atttggttcg ctcatggata
     6181 gatcctttgc tctatagaca gttaatgcgg tgccagtccg tagcgtttca tggtcaagct
     6241 attttactgc ttctgcagcc ggtggaagcg attccgtacg attgaggtat cctgcgaccc
     6301 taagtaacgg agttcgctgc catggccaag aatagcgaaa aagcaaagca catcgagtcc
     6361 gtcctgaatg aaaaccgcag ctttccaccc tccgccgaat tcatcgccag ggcacgtatc
     6421 aaagcctcag agctggcgca gctgcatcga cgcgcgcaag aggactacac cagattctgg
     6481 ggcgatctgg cacgccggtc attgcattgg cacaaacctt tttcaatcac gctcgacgac
     6541 agcaaggcac ccaactaccg ctggtttacc gacggcgaac tgaacgtatc ctggaattgc
     6601 ctggacgcac atctgggtga gcgcggtcac aaaaccgcaa tcattttcga aggtgagccg
     6661 ggggatagcc gacgtttgag ctatcgcgaa ctgcactccg atgtgtgccg cttcgccaac
     6721 gcgctgcgca aactcggagt gggtcacggt gaccgcgtgg tcatctatat gccgatggtc
     6781 ccggaggcgg tgatcgcgat gcaggcctgc gcgcgtatcg gcgcagttca ctccgtggta
     6841 ttcggcggct tctcggcgat cgcactgcgc gaccggattc gcgatgccga ggccaagctc
     6901 gcgattaccg cggacggcgg ctggcgcggc gggcacgcct tgaacctcaa gggtgcggtt
     6961 gacgcggcgt tggccgacgg ttgtccgacc atcaggtcgg tcgtggtcct gaagcgcacc
     7021 cacacccaga tcaccatgca tcccggtcgc gacgtgtggt ggcatgacct ggttgatggc
     7081 gaatcgccgc agtgcgagcc ggagtgggtc aatgccgaac atccgctgta ccttctctat
     7141 acctcgggat ccaccggcaa gcccaaaggc attcagcatt cgagcgccgg ctatctgctg
     7201 aacacccggc taacttgcga gtgggtgttt gatttgcgcg aagacgatgt cttctggtgt
     7261 accgcagacg ttggctgggt caccggtcac agctatgtcg cgtatggacc gctcgccgcc
     7321 ggcgccacca tcgtgatgta tgaaggcgca ccgatgattc ccgacggcgg acgcttctgg
     7381 aagatctgtg cggcgcacgg cgtgaccgtc ttctataccg cgccgactgc gattcgctcg
     7441 ctcatgaagc tgggcgatga gattccttct cgttacgatc tgtcgaagat ccgactgctg
     7501 ggcagtgtcg gcgagcccat caacccggaa gcctggatgt ggtatcaccg agtgatcgga
     7561 cgcggacgct gtccgatcgt tgatacctgg tggcagaccg agaccggcgc catcatgatc
     7621 tcgccattgc ccggcgtgac cgtcgccaag ccgggctctt gcacgcaggc gctgcccgga
     7681 atcttcgccg acatcgttga tgaccgcggc gaatcggtca aacagccagg cgccggcggt
     7741 tatctggtca tccgcaagcc ctggccgtcg ctggcgcgca ccatctggaa ggacaacgac
     7801 cgctatctga gcacctactg ggagaagttc aacaaccgtt tctacctcgc cggcgacggt
     7861 gcccatcgcg actcagaagg gtacttctgg atcgtcggcc ggatcgacga cgtgctcaac
     7921 gttgccggac atcggctcgg cacgatggag atcgaatccg cgctggtagc acatccgcgc
     7981 atcgccgagg cggccgtggt cggccgtccg cacgaggtca agggggagtc ggtgttcgcg
     8041 ttcgtcgtct gtcgtggcgc gcgcccggat gccgacctcg gcaagctcac cgaagagctg
     8101 cgttcctggg tcgctcagca actcagcccg atcgccaagc ctgacgacgt ccgctttgcc
     8161 gatgcgctgc cgaaaacgcg ctccggcaag atcatgcgcc ggctgctgcg cgcgatcgcg
     8221 cgcggtgaag agatcacgca ggatgtctcg acgctcgaga atccagccat cgtcgcgcag
     8281 ctgcgcggcg agaacatggc cgctcacgct gcgagtatca ggtcgagcac gcctgcgcca
     8341 cacaagccca aggccaggaa cacaaccaaa tcgaaagtca aggcgaaggc gaaggcgaaa
     8401 acgaaagtca cggccaaggc cggccgcaag ccggttcgcc ggctcaccgc gcgccaccgc
     8461 ccgccgagta aggcgaaggc caagacgaag gtttccaggc gcaaggtacg cgcgcgccga
     8521 tgaaatggcg cgtcgtttcc gcggccgcgc tggtttcggc ggggctgtgc ctgagtgcat
     8581 gtcgcacgcc tccggggccg aagacgactc cgggtgcgct cgttcaaccc gctgccccgc
     8641 caggtgcgtt ggtcccggga actcactaca cggtcgatgc gcagcgctcg caggtgttgg
     8701 tgctggtcta tcgcgatggt gcgatggcgc acctcgggca caatcacgtg atttctgtgc
     8761 ggcaactcag cggcagcgtg tcgatgggtt ccgacccggg ccactcatcg tttcagatcg
     8821 acttcccggt cgatgccatg agcgtggatg aagcggcgct gcgcgccgcg gaaggcgcgg
     8881 attttcagag cacggtcgat gaggcggcga ttgccggcac gcgcgatcac atgctgggcg
     8941 aagcgctgct caactcgaag cagttcccgc agattcacct cgcgtccgag cagattcatg
     9001 acagcggcgg cagtctgagt gccctcacca cgatcgtggt acgcaacacc acggctcatg
     9061 tgcagatacc ggtgacgcta cagacctccg gcgatgatct gactgtgaac ggtgagttcg
     9121 atctgaccca cacgcagctc ggattgactg catacagcgt cgcgctcggc gcgctgcgcg
     9181 tggccgagcg cattcacatc cggtatcgac tgatcgcgca ccgacaaccc taggagcctg
     9241 tccgcggtcg cggtcacgcg catggctcag acagcctcct agtcggatac tggcacgatc
     9301 agcagcgcgg tgccttccgg ggactcgatc agacagctac gctttcgatc cagaccgcgc
     9361 ggcaagtggg tcgatgtcgg tagctccagt tcgcgcatcc gctgcatcgc ggccgcaata
     9421 tcgacgcact cgaataccag caatggagcg tcgaacgtgc ggcgctggtg gaacgccaga
     9481 tccaggtgat cgctggtcaa cggcagatgc gggaacggta gatcttcttc cgccagcgcg
     9541 acgaaaccag cgagctccca gaatatacgg gcgctgtcga aatcggtctg cggcagactc
     9601 aggtgcgaga agtaaccgca caaggattgt ttggcagagc cctgcggtgc gggcgaatag
     9661 gtacgcgcct ccagcaaggt cacggcatgt ccggccggat cgcgcaacac cacttgattc
     9721 aggcggtcct cacccagcag ggccaggtcg ggttcaagcc gctgcgaacg caggcgcggc
     9781 agcgcgtgca ccagatcagg cagcacgaag gttaccgagg ggctggtcat ttcacgctcg
     9841 tgcagtccga cgtatagacg ctcgtcgctg agtaccccgt agcggtgagg ccacgcgtcg
     9901 gaggtcatca gctgcccgaa gccgaggcga tcatagaaag tgaacgacgc ggtgatgtca
     9961 cgagtcgcca gcccgatttc taggaaacgc ccaaacatta cgacgccggg gctcgcatgc
    10021 ctgacggggc tgaccagtcc gcgatgctgc gcgcgaccag ccgtgcgagc atcgcggcgt
    10081 ggtcggatcg ggcattgaga gccggcacgt agtcaaattg ctccccgccg gccttcagga
    10141 aaatttcgcg tccgaccagc gcaatctctt cgagcgtctc gaggcaatcc gaggcgaatc
    10201 ccgggcatac gaccctgaca gcgcgcacgc cgcgtcgcgg cagctgcgcc aatacctcgt
    10261 cggtcgcggg ggtgagccag cgattggcac caaaccgcga ctgaaaagtc aggctccagt
    10321 cgcgctgttc cagatccatc gcggccgcca gcagtgcggc ggtctggcgg cactcatcag
    10381 cgtagcgatc gccagcggtg acgtaagaca gcggaatgcc gtggaacgac atcagcaggt
    10441 gcccaccttt accgtgcagg cgccgatagt cggtaagact ggcagccagg gcgcgaatgt
    10501 agtccggctc ggcgtggtag ttggcgatat agcgcagctg cggtagcgcg cgccagtccc
    10561 gcaaggcctg tgcgacctga tcgtagacgg caccggtggt gctgccactg ctttgaggga
    10621 acagcggcag aaccagcagc cgacgcacgc cggcatcacg cagccggcgg atggtctggc
    10681 cgacgaacgg cgcggaatac aggaacgcct gttcgacctg cacgtgaccg cgcaacgcat
    10741 ggatctcacg ctccagagcc tcgcgcagct gcccggtgta gacgagcaag ggtgagcctt
    10801 cgtcgcgcca gatccgctgg tacttgtgcg cgctacgtgg tgagcgcacg ggcagtacga
    10861 gcccatacag cagcggcagc cacagcacgc gcggcaattc gatgacccgc ggatccgaca
    10921 ggaagcggcg caggaatcgg cgcaccgcgg cggtactggc cgcctcgggg gtaccggtat
    10981 tgaccagcag taccgcgggt gagacgcctc cggggttcag cgacgaatcg ttcacggcaa
    11041 ttcggctggc acgctaaccc acgcgctgtg ccagaaacgc cagggtacgg ctgcgtgcca
    11101 gggcggcatc ttccgcacgg tatcgatcgg ggcggtcttg gttggcgaag tcgggggctg
    11161 ccggatacag gtagtactcg cccgctggat aggcctcgcg cagctgcggc acttcgctgg
    11221 ccgcatggcc gctgtcgtgt tcgccgaaat gaaactgggt gggacaaggc ggtgtcttgg
    11281 gcgagtgctc gagcatcttc gcgccgtcat agcacacggc agccgccatc ggtaccgagc
    11341 cggcagccgc ccaggtcagc gtaccgcccc agccgaaacc gaccgtggcg acccggccgg
    11401 cgtggcgcac gaccgcggcg gcggcggcga tgtccagcag tgctttggcc aatggaacct
    11461 gcagccgata gcccatcgcc agctcggcgt cctcaggcgt gtaccccagc accacgttgc
    11521 gatgaatgcg gtcgaacagc gccggggcga tggccagata gccctcatgc gcaaaatggt
    11581 ccgcgacccg ccggatatga gcagtcagtc cgaagatctc ctgcaccacc accacggcgc
    11641 cgcgggcgcg atcgcaggca ctggcaaggt aggcgctgaa ggtatgccca tcgcgggcca
    11701 tcagcgactg gaattgattc acgcacgttc caggatcgcc gtcacaccca tgccgcccgc
    11761 cgtgcacgcc gaagcgatgc cgcgcttgga ttgcggagcg agcgatagct gcttcgccag
    11821 agtggccagg atgcgtgctc ccgtggctgc aaagggatga ccgatcgcga cactgccgcc
    11881 cttgagattg agcttggcat agtcgattga accgagtgct tccgaaagtc ccaggcgatc
    11941 ccggcagtaa tcagccgact cccacgcttt gaacaggcac agcggcaccg ccgcgaacgc
    12001 ttcatggatc tcatagaaat cgaagtcctg cagtctcagg ccggtatctt tgagtatttc
    12061 ggccacggca tacgcaggcg ccatcagcag cccctctttg ccgctggcga aatccaccgc
    12121 ccagctcttg ccggccgtta tgtaggccag gatcggcaag ttacgccggg cggcccactg
    12181 ctcggaggcg agcagcagtg ccgcggaccc gtcagtcagc ggtgtgctgt tgccggccgt
    12241 gagactgccc tggccgctga agtcgtaaac ggccttcagc ttgcccagtc gctccaccga
    12301 ggtatcggtg cgcacattgt tgtcagtgtt cagatcacga aacgggatca ccagatcctc
    12361 gtagaaaccc tcgcgccagg ccgtagcggc cttgacgtga ctgtcatacg caatgcgatc
    12421 ctgatcttcg cggctgatgt tccaggtctt gaccatcagc tcgcagctct gccccatcga
    12481 cagacctgtg cgaggttcca ccactgcggg caacaccggt ttgaaatcac gcgggcgcag
    12541 actgagccag ggtctcatgc gctgaccgaa gctgcggccg cgcgcgcttt gcaggagttt
    12601 ctgctgaaag gacctcggat aggcgatcgg gggatcgctg gtggtgtcga caccggcggc
    12661 gataccggcc tcgatctgcc ccagcgcgat cttgttggcc accaggatgg ccgcctcgag
    12721 gctggtgccg caggcgcgtt gcacgtccaa tccgggcgtt tgcggatcga gcccggaaga
    12781 cagcaggctt tcgcgcgtca ggttatagtc ttttgggtgt ttgatgacgg cgccggcaat
    12841 gacatcgccg aggcgttcgc cgtgcagatt gaagtgatcc acgattccgc gcagggaagc
    12901 gatcaacagc tcctgatcgt cggagctggc ataagccgta ttggagcgtg cgaacggcgt
    12961 gcgtattccg ccgatgatcg cgacgcgtcg ggtggtgctt cctacctgca tgattggcga
    13021 ctccgtgaaa acgcctagga tgccatgccc gtgcgggcgc ggcgacagat ggccatcttg
    13081 cataacaacg agcattgcgg gagtgacggg ctaaagtgct tgatcgtgta tacgagcgcc
    13141 gtctcgccgg actgatcaac agtcgtgagc agcgcctgct ttgcgaaggg ccgcgcgggc
    13201 tggaacgcga gtcgttgcgt gtccatccgg atgggcgtat cgcccagacc ccgcacccgg
    13261 tcgggctggg atcggccctc accaatccgc acgtcacgac cgattacgcc gaagcgctga
    13321 cggagctggt cacaccgacc tttcagagca atgctgcgtt gctgcagtac ctgcaggagc
    13381 tgcatcagtt cgtctaccgc cggatcgctg acgagctgct atgggcgact tcgatgccct
    13441 gcgcgttgcg gggcgatgcc gacgtcccga tcgcccgcta cgggccgtcc caccagggac
    13501 ggctgaagtc gatctaccgg cacgggctgc tgatccgcta cggcgggatc atgcaggcga
    13561 tctccggcgt gcatttcaat tactccttcc cgcagcagtt ctggccgctc tatgcgcagg
    13621 tgtatcagtc gcatgacgaa ggccaggact tcgtgtcggc gcgctacttc gacatgctgc
    13681 gcaactatcg tcgccatggc tggatcgtct cctatctgtt cggcgtgtca cccgctttgt
    13741 gtcgctcgtt cctgcagaat cgtcaggatc accagctcga gccgctggga gacgatactt
    13801 tcatcggtcc gcatgccacc tcgcttcgca tgagtgacat cggctaccga aatcgcggcc
    13861 agagcgaggt cgcggtctcg gtcaaccata ttgatgagta tctgcgtgac ctgcgcaggg
    13921 cggtgaccac accccacccg ccgttcctga agctgggcgt caaggtggac ggcgaatacc
    13981 gccagctctc cgggaatctg ctgcagatcg agaatgagta ctacagctac gtgcgaccca
    14041 agcgcgcgct gcgcgcaggg gaacgcacga ctcacgcgct ggctcgtgcc ggggtcgagt
    14101 atgtcgaggt gcgggcgctg gacaacagcg cgttcgatcc ggtcggggtc aacctgcgca
    14161 agctctactt cctcgaggcg ttcgttcagc tcctgatgtt caagtccagc gcgccgatcg
    14221 atgcgagtga agaagagtcg atcgaccgca atcatctgct cgtggcgcgt catggccgcg
    14281 aacccgggct gacgctggtt cgcgacgggc gcaacgtgcc gatgatcgga tgggccgagg
    14341 agctgctgga ttcgatgacg ggcatttgcg agctgctgga cagcggacac cctgaacggc
    14401 cctacagcgc aacccttaag gatcaacagg ccaagctcat ggacgtcgag cagacaccgt
    14461 ccgcacggtt gctgcgcgaa ctgcgagagc acggcgagag cttttccgcg ctagcactgc
    14521 gctattccac cgagcaccgc cgttccatcg atgcgcaacc gaccaacgat cggcgcagcc
    14581 aggagttcgc ggcagaagcc gagcagtcgc tggaagcgca gcgctccctg gagttctcgc
    14641 aacagggcag cttcgatgaa tacctggcgc gctatctggc cgattgaaat gttcagcacc
    14701 agacatgggc gtatattcgg cgaccgcgcg ccgtgatggt gccgtgagcg gtaccatttg
    14761 cgcctggatg agggcactca cgtggcacga aaggtgcacc ttcggatcct gcctagcctg
    14821 gggatcgaac aagaattgag gacgggagct ctcgcatgaa actggtcacc gcaatcatca
    14881 agcccttcaa actggacgat gtgcgcgcgg cgttgtctga gcttggcgtg tcaggcatga
    14941 ccgttaccga ggtcaagggc tttggccgcc agcgcgggca cactgagctg tatcgcggtg
    15001 ccgaatatgt cgtggatttt gttccgaaaa cacgcgttga agtcgcggtt cgcgccgaac
    15061 tggtcgacca ggtgattgag gccgtgctga aggcg
//
LOCUS       DATMRM010000471         3372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1211370, whole genome shotgun sequence.
ACCESSION   DATMRM010000471 DATMRM010000000
VERSION     DATMRM010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211370"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VNZ06_14365"
     CDS             <1..1004
                     /locus_tag="VNZ06_14365"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HWW21981.1"
                     /translation="PDPESGSGTLALDDTWALSPALRDTIGALYQQRQVAFVPFAGTD
                     DLTRSHFETQDSIELGQPLGGRHDFRSGFMARLSNSLLAGSTPPAPIAFTDALPLCFQ
                     GPAQIPNLSLKNVGKPPFDERQSRILAGMYAGHSLQSAVSNGLELRQEVAQAMADEMT
                     AANRNAINSKGFELEAERMGRLMRDNYRIGFIDIGGWDTHVNEGAAQGALATNLAGLG
                     RGLQAFAQSLGSEWNNTVVVVLSEFGRTFRENGNRGTDHGHGTVYWVLGGAIAGGRIA
                     GEQQPLERVNLFQDRDYPVLNDYRALLAGLFRSMWSLTAAQLEPVFPQARSIDLKLL"
     gene            complement(1013..1525)
                     /locus_tag="VNZ06_14370"
     CDS             complement(1013..1525)
                     /locus_tag="VNZ06_14370"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HWW21982.1"
                     /translation="MSNALTAANPPPLPRKIDGLRLIALFKFAKVLLLIATSYGVHKL
                     LDANIIERLYSWSSTLSDDRLERKLIERALDWVEDLGAKRIQIFVAVTVAYTAVALIE
                     GIGLWMRKTWAEWLTTVFTASLIPFELWELVKRPPGHRLPIAATLLVNLIIVAYLVWF
                     LRRQQQAARH"
     gene            complement(1518..2369)
                     /gene="sseA"
                     /locus_tag="VNZ06_14375"
     CDS             complement(1518..2369)
                     /gene="sseA"
                     /locus_tag="VNZ06_14375"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HWW21983.1"
                     /translation="MAGDTLKALVSTDWLAGELGAADLRVFDASWYLPTEQRDAKAEY
                     ERAHIAGARFFDIDAVADTQSSLPHMVPTTAQFERMIGALGVCNASRIVFYDQKGLFS
                     APRAWWLMRLFGHEQCAVLDGGLPKWRAEDRALQQTQAPAPAVTSYRAAFNARRLRGL
                     GDVLANVTTRRELLLDARSAERFHARVPEPRPGLRGGHVPGSRNLPFSELLTPQQTLL
                     PAGELKARFQASGVDASREVITSCGSGLTAAILSLALHEAGLPMGALYDGSWSEWGAR
                     QDTPIDV"
     gene            complement(2382..>3372)
                     /locus_tag="VNZ06_14380"
     CDS             complement(2382..>3372)
                     /locus_tag="VNZ06_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21984.1"
                     /translation="VACGQITQARRIGDALLWAQGHDRFWHDGRLRNAYAAGVVRTDT
                     VKLAGWWDPNARKWLEDRYQVGSDTGNVAWAMLALLTLEKNTGEARYLDGALQLARWL
                     QGRADARGAGGFTGGDFGHEPTPQRLSWKSSEHNTDLAAAFHWLARATGDVQWQDRAR
                     AAQNFVIAMWRADTGYFAVGTADDGVTLNLLLALDAQIWPLMAIPGAATQYADALGTC
                     DRRLRFQTGYTYSQAGGGLWSEGTAQVAVLLQLLHRDSAARAVQASLQTQRAQDGGYY
                     ASDVPATPTGFMLATDPSKPRVYFHLEHLGAAAWAALSEQRFNPFTGRSELAQ"
BASE COUNT          611 a         1137 c         1039 g          585 t
ORIGIN      
        1 agccagatcc cgaatcaggc agtggcacgc tggcactcga tgacacctgg gcgttgagcc
       61 cggcgctgcg cgacacgatc ggcgcgctgt accagcagcg tcaggtcgcc ttcgtcccgt
      121 ttgcgggcac cgacgatctc acgcgcagtc atttcgaaac tcaggacagc atcgaactcg
      181 gccagccctt gggcggccgc catgacttcc gctccggctt catggcgcgg ctgtccaatt
      241 ccctgctcgc cggcagcacc ccaccggcgc ccatcgcatt caccgacgcg ctgcccttgt
      301 gtttccaggg gccggcccag attccgaacc tatcgctcaa gaacgtcggc aagccgccat
      361 tcgacgaacg ccagtcgcgc attctggccg gaatgtatgc cggccattcc ctgcaatcgg
      421 cggtttccaa cgggctggag ctgcgccagg aagtcgctca ggccatggcc gatgagatga
      481 cggcagccaa tcgcaacgcc atcaacagca agggcttcga gctggaggcc gagcgcatgg
      541 gcaggctgat gcgggataac tatcgcatcg gcttcatcga catcggaggc tgggacacgc
      601 acgtcaacga aggcgccgcg cagggcgcgc tggcgaccaa tctggcaggc ctgggccggg
      661 gcctgcaggc tttcgcgcag tccctgggat cggaatggaa caacaccgtg gtcgtcgtgc
      721 tgtccgagtt cggacgcacg ttccgcgaga acggcaaccg cggtaccgac cacggccacg
      781 gcacggtcta ctgggtcctg ggcggcgcca tcgccggggg tcgcatcgcc ggtgaacagc
      841 agcccctgga gcgtgtgaac ctgttccagg atcgggacta tccggtgctg aatgattatc
      901 gcgcgctgct cgcgggctta ttccgttcga tgtggtcgct gactgcggcg cagctcgaac
      961 cggtcttccc acaggcccgc agcatcgatc tgaagctgct ctgacctggg ctctagtgac
     1021 gcgccgcctg ctgttgtcgg cgcagaaacc acaccaggta ggcaacgatg atgagattga
     1081 ccagcagcgt ggccgcgatc ggcaggcgat gtcccggcgg tcgcttgacc agctcccata
     1141 gttcaaacgg gatcagggaa gcggtgaaca cagtggtgag ccattccgcc caggtcttgc
     1201 gcatccacaa cccgatgccc tcgatcagtg cgaccgcggt ataggcgaca gtgaccgcaa
     1261 cgaagatctg aatgcgcttc gcgcccagat cctccaccca gtcgagcgcg cgttcgatca
     1321 gcttgcgctc cagccgatcg tcactgagag ttgacgacca ggagtacagc cgctcgatga
     1381 tattggcatc gagcagcttg tgcacaccat agctggtcgc gatcagcaac agtactttgg
     1441 cgaatttgaa aagcgcgatc aggcgcagcc cgtcgatctt gcgcggcagc ggaggcggat
     1501 tggcggcggt gagtgcgtta gacatcgatc ggtgtgtcct ggcgtgcgcc ccactcactc
     1561 cacgaaccgt cgtacagcgc acccatcggc agaccggcct cgtgcagtgc cagactcagt
     1621 atggcggcgg tgagtcctga gccgcagctg gtaatgactt cacggctggc gtcgacgccg
     1681 ctcgcctgaa agcgcgcctt gagctcgccg gcgggcaaca gggtttgctg cggcgtcagc
     1741 agttcgctga acggcaggtt gcggctgccc ggcacatgcc cgccccgcag tccgggccgt
     1801 ggctccggta cgcgcgcatg aaagcgctcg gccgagcgtg cgtcgagcag cagctcgcgc
     1861 cgcgtggtca cattcgccag cacatccccc aacccacgca ggcggcgggc gttgaacgcg
     1921 gcgcgatagc tggtgacggc cggggccgga gcctgtgtct gctggagcgc gcgatcctcc
     1981 gcgcgccact tgggcagccc accatcgagc accgcgcact gctcgtgtcc gaatagtcgc
     2041 atcagccacc aggcgcgggg cgcggagaat aggcccttct ggtcgtagaa cacgatgcgg
     2101 ctggcattgc agactccgag cgcgccgatc atccgctcga actgcgcggt cgttggcacc
     2161 atgtgcggca atgaagactg cgtgtcggcc accgcatcga tgtcgaagaa tcgcgctccg
     2221 gcaatgtgcg cgcgctcata ctcggctttg gcatcacgct gctcggttgg caggtaccag
     2281 ctggcatcga acacgcgcag atccgctgcg ccgagctccc ccgccagcca atccgtgctc
     2341 accagtgcct tcaaagtatc ccccgccatt gaaaccttta ctcactgtgc caactcagag
     2401 cgacccgtaa acggattgaa gcgctgctcc gatagcgccg cccaagcagc tgctcccaga
     2461 tgctcgagat gaaaatagac ccgcggcttg ctcggatcgg ttgccagcat gaatccggtc
     2521 ggcgttgccg gtacatcgct ggcgtagtag ccgccatcct gtgcgcgctg cgtctgcaat
     2581 gacgcttgca cggcccgcgc ggcgctgtcc ctgtgcaaca actgcaacag taccgcgacc
     2641 tgcgcggtac cttcgctcca caaaccgcca cccgcctggc tgtaagtata tccggtctgg
     2701 aagcgcaatc gccgatcgca ggtgcccagc gcatcggcat attgagttgc cgcgccgggt
     2761 attgccatca gcggccagat ctgcgcgtcc agcgcgagca gcaggttgag cgtgacgccg
     2821 tcgtccgcgg ttccgactgc gaaatagccg gtatccgcgc gccacatggc tatcacgaag
     2881 ttctgcgcgg cgcgtgcccg atcctgccac tggacgtcgc ccgtggcgcg cgccagccaa
     2941 tgaaacgccg ctgccagatc ggtattgtgc tccgaggact tccacgacag gcgctgcgga
     3001 gtcggttcgt gaccaaaatc gccaccggtg aaacctccgg caccgcgcgc atcggccctg
     3061 ccttgcagcc aacgcgcgag ctgtaaagcc ccgtcgagat agcgtgcctc gcccgtgttt
     3121 ttttccagcg tcagcagcgc cagcatggcc caggcaacat tgccggtgtc gcttccgacc
     3181 tgatatcgat cctccagcca cttccgtgca ttgggatccc accagcctgc cagcttcact
     3241 gtgtcagtgc ggacaactcc cgcagcgtac gcattgcgca aacgcccatc gtgccagaag
     3301 cgatcgtgac cctgcgccca cagtagtgca tcgccaatgc ggcgcgcctg agtgatctga
     3361 ccacaggcca cc
//
LOCUS       DATMRM010000472         2832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1211407, whole genome shotgun sequence.
ACCESSION   DATMRM010000472 DATMRM010000000
VERSION     DATMRM010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211407"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..226)
                     /locus_tag="VNZ06_14385"
     tRNA            complement(151..226)
                     /locus_tag="VNZ06_14385"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(191..193),aa:Phe,seq:gaa)
     gene            complement(283..558)
                     /locus_tag="VNZ06_14390"
     CDS             complement(283..558)
                     /locus_tag="VNZ06_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HWW21985.1"
                     /translation="MNRTVNCVLLKREAPALDRPPYPGPLGQRIFQNVSKEGWERWRA
                     HQIMLINEYRLTPIEPKARKFLEGEMEKFFFGAGSSKPGEFVEPERK"
     gene            complement(555..1595)
                     /gene="mutY"
                     /locus_tag="VNZ06_14395"
     CDS             complement(555..1595)
                     /gene="mutY"
                     /locus_tag="VNZ06_14395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HWW21986.1"
                     /translation="MDFAQTLIDWHLRHGRHDLPWQHHKTPYRVWVSEIMLQQTQVAT
                     VVGYYERFVHRFPDVETLAAAPIDEVLHLWAGLGYYSRARNLHRASQIIVQAHRGEFP
                     GEVEVLAELPGIGRSTAAAVVSIAFDRRATILDGNVKRVLARYFAIEGAPNERRVEQE
                     LWQRADGCTPDSQVAIYTQAIMDFGATLCTRHDPLCMHCPLRKGCKAFAAGRVSDLPT
                     PRKRPNRPTRQVVMLFAVNGRGAVLLQRRPARGIWGGLWSPPEFDSAEAATRFCKSEL
                     RSEPSHTECMSLVRHAFTHFDLEITPLRVSLAGPPLETAGEGALWYNARNPERIGLPA
                     PIATLLAGMELQ"
     gene            complement(1618..>2832)
                     /locus_tag="VNZ06_14400"
     CDS             complement(1618..>2832)
                     /locus_tag="VNZ06_14400"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWW21987.1"
                     /translation="LDIDAQKRAEVALRASEARLQTAVWGARMGLWETELTHDTTRWF
                     DSWCAQYGIDPCEGQKHQERWLANVHPSDAAGAAQRYQDHLEGRQDFYDSEYRIMTAE
                     GQWRWIYARARVTERDESNAPLRMVGVCMDVDARRRAELQKHFTQPWLEAALQVARCG
                     MWHWQAEPFEVTYTDSYYRLFGVDPIAGRAQLRFWYNNIHPDDRERAMTIANNMVAGK
                     TESYEQEYRMRCADGSWMWVLDRACAMVRDEKGGGKQMVGFVIDFTERRAQREALRAS
                     DELFRHATMAARGMVFEVDFVSGWVNRFGSERLLGYTSEELGHSREEWVARIHPEDVG
                     CFDAMRPVDTAEGHTAVIEYRVKHKAGHWVWLRGSGVTLTDGNGKPTRRIGFLQASEA
                     SEHPIPPQGSNS"
BASE COUNT          516 a          936 c          835 g          545 t
ORIGIN      
        1 ggctggtgag ctttatgcag tacgatctgg gcttctttga tcacgagacc gggcggataa
       61 ccagcgccga gaaccccttc ggcgcaaaag tgttacccat gtgcccggaa tgaaacgtta
      121 ccgatgtggt cggaacggac cgcaatttga tggtggccag gggtggaatt gaaccaccga
      181 cacgcggatt ttcagtccgc tgctctacca actgagctac ctggccgtcg gaggggacgc
      241 gtattagacc ggtcggccgg cgggaccgtc aaccccttga ctctacttgc gttccggctc
      301 gacaaattcg ccgggtttgg atgatccggc tccgaaaaag aacttctcca tctccccttc
      361 caggaacttg cgggccttgg gctcaatcgg tgtcagccga tattcgttaa tgagcatgat
      421 ctggtgcgct cgccagcgct cccagccctc tttggagaca ttctggaaaa tgcgctggcc
      481 cagagggcct ggataaggtg ggcggtccag tgccggcgcc tctcgcttca gcaacacgca
      541 attgacagtt cggttcattg cagctccatc ccggccagca gtgtcgcgat gggcgccggc
      601 agcccaatcc tttcagggtt tcgtgcgttg taccacagcg ccccctcacc ggcggtctcc
      661 aacggcgggc cagcgagact tacacgaagg ggcgtgatct ccagatcaaa gtgcgtgaat
      721 gcatgacgca ccagggacat gcactcagta tgcgatggct cgctgcgcaa ttcgctcttg
      781 cagaaacggg tcgcagcctc agcgctgtcg aactccggcg ggctccatag ccctccccag
      841 atgccgcgtg ccgggcgcct ctgcagcagc accgctcccc gtccattgac cgcaaacagc
      901 ataacgacct gacgcgtcgg gcgattcgga cgctttcgcg gcgtgggcaa gtccgacacg
      961 cgaccggcag cgaaggcttt gcatcccttt cgcaacggac agtgcatgca caatggatcg
     1021 tgtcgggtac acagggttgc accaaaatcc atgattgcct gggtatagat ggcaacctgc
     1081 gaatcaggcg tgcacccgtc ggcgcgttgc caaagctcct gctcgacgcg gcgttcattg
     1141 ggtgcccctt cgattgcgaa gtagcgtgcc agaacgcgct tgacgttgcc gtccagaatc
     1201 gttgcgcgcc ggtcgaaggc gatcgagacg accgccgcgg cggtcgaccg gccgatccct
     1261 ggaagctccg cgagcacttc aacctcgccg ggaaactcac cgcggtgcgc ttgcacgatg
     1321 atttgcgatg cgcggtgcag attacgcgca cgggagtagt agccgagccc ggcccacaga
     1381 tgcagcacct catcgatcgg agctgcagcc agagtctcta catccggaaa acggtgcacg
     1441 aagcgctcgt agtagccgac gaccgttgcc acctgcgttt gctgcagcat gatctcggag
     1501 acccacacac ggtacggcgt tttgtgatgc tgccacggaa ggtcgtgacg gccgtggcgc
     1561 agatgccagt cgatcagagt ctgggcgaag tccatccgcc cattctcgcc gcttcgctca
     1621 gctgttggaa ccctgcgggg gaatcgggtg ttcggacgcc tcgctcgcct gcaggaagcc
     1681 aatgcggcgc gtcggcttgc cattgccatc ggtcagcgta acacccgagc cccgcagcca
     1741 gacccagtgt cccgccttgt gcttgacgcg atattcgatc accgcggtgt gaccctcggc
     1801 cgtgtcgacc ggacgcatgg cgtcgaagca gcccacatcc tccggatgaa tgcgcgccac
     1861 ccactcctcg cggctgtgtc ccagttcttc actggtatag ccgagcagcc gctcactgcc
     1921 gaagcggttg acccagccgc tgacaaagtc gacctcgaac accatgccgc gcgcagccat
     1981 cgtcgcatga cgaaacagct catcgctggc gcgcagcgcc tcgcgctggg ctcgccgttc
     2041 ggtaaagtcg atcacaaagc cgaccatctg tttacccccg cccttctcgt cgcggaccat
     2101 cgcgcaggcg cgatccagca cccacatcca gctgccgtcg gcgcagcgca tgcggtattc
     2161 ctgctcatag ctttcggtct taccggccac catgttgttc gcgatcgtca tcgcacgctc
     2221 tcggtcgtcc ggatgaatgt tgttgtacca gaagcgcagc tgcgcgcgcc cggcaatggg
     2281 gtcgacgccg aacaggcggt aatagctatc ggtgtatgtg acttcgaacg gctcggcttg
     2341 ccaatgccac atcccgcagc gcgcgacctg cagcgccgcc tcgagccacg gctgggtaaa
     2401 atgcttttgt agctccgctc ggcgccgcgc atccacgtcc atgcacacgc cgaccatgcg
     2461 cagcggcgca ttgctctcat cgcgttcggt cacgcgtgca cgcgcataaa tccagcgcca
     2521 ctgcccctcg gcggtcatga tgcggtattc ggagtcgtag aagtcctgcc gcccttccag
     2581 atgatcctga tagcgctgcg cggcaccagc ggcatcgctc ggatgaacgt tggccagcca
     2641 gcgttcctga tgtttctggc cctcgcacgg atcgatcccg tactgcgcgc accagctgtc
     2701 gaaccaacgt gtcgtgtcgt gcgtcagttc ggtttcccac agacccatac gagcgcccca
     2761 gacggctgtc tgcagccgcg cctcgctcgc acgcagcgcc acttcggcgc gtttctgcgc
     2821 atcgatgtcg ag
//
LOCUS       DATMRM010000473         2636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3046004, whole genome shotgun sequence.
ACCESSION   DATMRM010000473 DATMRM010000000
VERSION     DATMRM010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3046004"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VNZ06_14405"
     CDS             <1..676
                     /locus_tag="VNZ06_14405"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HWW21988.1"
                     /translation="FMQFLEHRAANAEALYILGDLFETWVGDDDDEPYRNRICRSLAA
                     LSSWGVPCYVMHGNRDFLFQSGFEQRTGARLIADPVVTELYGEQVLLTHGDALCIADR
                     PYQRLRGIVRNPRWQRRFLRLPLSLRRGLADRARRGSQRHTQRTAYEIMDVDLPAVSE
                     AMRACGVRTLIHGHTHRPAVHGFQLDGQDARRIVLGSWHQAGSCLEWGAQGFQLLALP
                     RRGGPE"
     gene            complement(637..1248)
                     /gene="miaE"
                     /locus_tag="VNZ06_14410"
     CDS             complement(637..1248)
                     /gene="miaE"
                     /locus_tag="VNZ06_14410"
                     /inference="COORDINATES: protein motif:HMM:NF017939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HWW21989.1"
                     /translation="MNILAAVTPQAWVDAACEGWRELLVDHANCEKKAASSALAMIFA
                     YPQDQALTTTLSRLAREELRHFEQVSQMMRRLQVEYVRQRPGRYAAGLRAAVRQHEPV
                     RKLDLMCAHALIEARSCERFRLLAARLPAPLAAFYESLRHCEARHFELYVRLAQLAAD
                     AADPSQAQTRLAELAAVEAELATLPDEQLRFHSGPPRLGSASN"
     gene            complement(1298..2566)
                     /locus_tag="VNZ06_14415"
     CDS             complement(1298..2566)
                     /locus_tag="VNZ06_14415"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWW21990.1"
                     /translation="MGIRVLLIEEDPKVRALIRHHLSCEWPEAQLTAYPPQQRGPLPA
                     EYLAQGHDAVLLSADANDGHGLMWLRDLSSRRGFAPIIYLLEDDDDQERQRERAMESG
                     AFGVASKGRMDHDGFIRLLEAASRQHRREMADWRVSPEALESRRFGEARIPGYRRARL
                     LARGSVSQLYVAESEKAGTLVVLKVTPSRRDDSGVDQAFARFLQEYEIAQRVHHPNIV
                     RLYELGVADDHAYLAMEYFTHGDLRRRMRAGISPSEALGFASQIARALGALHSAGILH
                     RDLKPGNVMLRADDQIALIDFGLAKHEALELEITDTGLIFGTPHYMSPEQGHGQDIDL
                     RSDLYSLGVMLFEMLSGQKPYTATNPMAIIYMHRNAPLPRLPEPLADMQPLLDRMMAK
                     EPADRFVDALETARQIDSTRNAWLLKGALV"
BASE COUNT          439 a          880 c          852 g          465 t
ORIGIN      
        1 cttcatgcag tttctggagc atcgggccgc gaacgccgag gcactgtata tcctcggcga
       61 tctgttcgag acctgggtgg gggatgatga tgacgagccc tatcgcaacc gcatctgccg
      121 ctcgctggcg gcactgagta gctggggcgt gccgtgctat gtcatgcacg gcaatcgcga
      181 ctttctgttt cagagtggct tcgagcagcg caccggcgct cgcctgatcg ccgatccggt
      241 cgtgaccgag ctgtatggcg agcaggtgct gctgacacac ggtgatgcgc tctgcatcgc
      301 cgaccgcccc tatcaacgtc tgcggggcat cgtgcgaaat ccgcgctggc agcgcagatt
      361 cctgcgtctg ccgctgtcgt tgcgtcgcgg attggccgat cgcgcacgcc gcggcagtca
      421 gcgtcacacg cagcgcaccg cctacgagat catggatgtc gatctgcccg ccgtcagtga
      481 ggcgatgcga gcctgcggtg tgcgcacgtt gattcacggt catacgcacc ggccggcggt
      541 gcacgggttt cagctcgacg gacaggacgc gcgccgcatt gtgctcggct cctggcacca
      601 ggccggcagc tgtctcgagt ggggagcgca gggatttcag ttactggcgc tgcccagacg
      661 cggcggaccg gagtgaaagc gcagctgctc atccggcaga gttgccagtt cggcttccac
      721 cgctgccagt tcagccagcc gtgtctgcgc ctgcgacgga tccgccgcat cggccgcgag
      781 ctgtgccaga cgcacgtaca gttcgaagtg gcgcgcctcg cagtgccgca gtgattcgta
      841 gaaagccgcg agcggtgcgg gcagacgcgc ggccagcaac cgaaagcgct cgcacgaacg
      901 cgcctcgatc agtgcgtgcg cgcacatgag gtccagcttg cgcaccggct cgtgctggcg
      961 cactgccgcc ctcaggcccg cggcatagcg accggggcgc tggcgcacat actccacctg
     1021 caggcgccgc atcatctgtg acacctgctc gaagtgccgc agctcctcgc gcgccagccg
     1081 cgacagtgtg gtggtgagag cctgatcttg cggataggcg aagatcatcg ccagcgcgct
     1141 ggacgcggcc ttcttttcac agttggcgtg atcgaccagc agttcgcgcc aaccttcgca
     1201 cgcggcatcg acccacgcct gcggtgtcac ggccgccaaa atgttcatgc cgatgtccta
     1261 cccgcgcgag cagccgaggc cggcgccgcg aggaccttca caccagagct cctttgagga
     1321 gccaggcgtt gcgggtcgag tcgatctggc gcgcggtctc gagggcatcg acgaagcggt
     1381 cggctggctc cttggccatc atccgatcga gcagcggctg catatcggcc agcggctcgg
     1441 gcaggcgcgg caacggcgca ttgcgatgca tgtagatgat cgccatcgga ttggtggcgg
     1501 tgtaaggctt ctgcccgctc agcatctcga acaacatgac gcccaggctg tacaggtcgc
     1561 tgcgcagatc gatgtcctgg ccatgcccct gctccgggct catgtagtgg ggtgtgccga
     1621 agatcagacc ggtatcggtg atctccagct ccagcgcttc gtgcttggcg aggccgaagt
     1681 cgatcagcgc aatctgatca tcggcgcgca gcatgacgtt gccgggcttc agatcccgat
     1741 gcaggatgcc ggccgaatgc agcgcgccca gtgcgcgcgc gatctggctg gcaaacccca
     1801 gagcctccga gggcgatatg ccggcgcgca tccgtcgtcg cagatcaccg tgcgtgaagt
     1861 attccatcgc caggtacgcg tgatcgtcag cgaccccgag ctcgtacagc cgcacgatgt
     1921 tcggatggtg gacgcgctgc gcgatttcgt attcctgcag gaagcgcgcg aaggcctgat
     1981 ccacgcccga gtcatcgcgc cgcgagggcg tcactttcag caccaccagc gtgccggctt
     2041 tctcgctctc ggccacgtag agttgcgaga ccgagccgcg cgccaacaat cgcgcacgac
     2101 ggtaaccggg aatgcgcgcc tcaccgaagc gacgcgattc cagcgcctcg ggcgagaccc
     2161 tccagtcggc catctcccga cggtgctgcc gcgatgccgc ctcgagcagc ctgatgaaac
     2221 cgtcgtggtc catccgtcct ttggaggcca ccccgaacgc gccactttcc atcgcgcgct
     2281 cacgctgacg ctcctgatca tcgtcgtctt cgagcagata gatgatcggg gcgaagccgc
     2341 ggcggctgct cagatcacgc agccacatca atccgtgtcc gtcgttggcg tctgccgaca
     2401 gcagcaccgc gtcatggccc tgagccagat actccgcggg cagcggtccg cgctgctggg
     2461 gtggataggc ggtgagctgc gcctccggcc actcgcagct caggtggtga cggatcagtg
     2521 cccgcacctt gggatcttcc tcgatcagca atacgcgtat gcccactcga gcgcccccgg
     2581 aagctgacaa cgagcttata gcccgccgat cgctttcaga cccatgcggg ctgcgc
//
LOCUS       DATMRM010000474         9009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_605781, whole genome shotgun sequence.
ACCESSION   DATMRM010000474 DATMRM010000000
VERSION     DATMRM010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9009
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605781"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1527
                     /locus_tag="VNZ06_14420"
     CDS             103..1527
                     /locus_tag="VNZ06_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWW21991.1"
                     /translation="MFKYKSRSLQAAFAVLAAALLVLALGKPLTATADEAPAAAATAP
                     APAAAPAATPAATTPAAPTEPQIVSFDKLNSGDTAWMLTSTALVLMMTIPGLALFYGG
                     MVRKKNVLATLMQSFAITCLVTILWWAIGYSLAFTPGSKYLGGFSRLFMHGVLYDHDG
                     MKVSVSHLALTIPETVYVMFQATFAIITPALICGSFAERMKFSAMMWFMGIWLLVVYC
                     PVAHWMWESTSGWFASTQLDFAGGTVVHINAGIAGLATALMLGKREGFGKEKMAPHNL
                     TLTVIGASLLWVGWFGFNAGSAVASDGRAGMAFAVTHIATATAALAWMFTEWLSKGKP
                     SVLGIASGAVAGLVAITPASGFVDPGSSVIIGVAAGVICYIASTAVKKMFGYDDSLDA
                     FGVHCIGGIVGALLTGALVSKLISGAEGSVLLQLKGVSATLVWSFVMSMIILKLIDLT
                     IGLRVTTEQEREGLDISQHGESIE"
     gene            1547..1885
                     /locus_tag="VNZ06_14425"
     CDS             1547..1885
                     /locus_tag="VNZ06_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWW21992.1"
                     /translation="MKLITAIIKPFKLDEVRTALTTLGVSGMSVTEIKGFGRQRGHTE
                     LYRGAEYKVDFVPKSRVEIAVRAELVDQVVEAIINAAKTGKVGDGKIFISDLQRVVRI
                     RTGETDDSAL"
     gene            complement(1960..2280)
                     /locus_tag="VNZ06_14430"
     CDS             complement(1960..2280)
                     /locus_tag="VNZ06_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW21993.1"
                     /translation="MNNRYLTLLAMGLLTASNVYGSTLCDDQLMTSLHECEHIVGSLR
                     PDKMGQMRVFASDGSEFTAGQAQWMKSQLKQVAAECAGGNVADASRQLAQVKDLLAQH
                     HRAG"
     gene            complement(2416..2856)
                     /locus_tag="VNZ06_14435"
     CDS             complement(2416..2856)
                     /locus_tag="VNZ06_14435"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWW21994.1"
                     /translation="MYQRILVAIDGTDSSSRACTEAINLAVDQKARLRLLHVVSYAYV
                     SAVLGGASSGDVMRRLREDGQDVLSAAAAPARAAGLEVEPRLFEHHSTQIGEGIIEDA
                     QVWHADLIVMGTHGRRGFARAVLGSDAEFVARHAPVPVLLVRKA"
     gene            complement(2885..4402)
                     /locus_tag="VNZ06_14440"
     CDS             complement(2885..4402)
                     /locus_tag="VNZ06_14440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWW21995.1"
                     /translation="MSDVRAALLVASATLAASCAVGPRYHRPGAPANPGYASEPLPQS
                     SASAPVHAGETQRLISGRDIPFKWWEQFQSPALNSLVERAFKANPSIAAAQAALIQAQ
                     ELVYAQRGYFFPTLGANYNFARTKIAGNFTVDDSPGTQGDGHNLNPPLLDLQNSPHTA
                     PLIYNYHTAQLTVGFVPDLFGANWRQMESLAAQTQAQRFALEATYITLASNVVAAAIQ
                     EASLRAQIGATRDIIAADENSLRILRNQLQLGFAMRIDVAAQETALAQARANLPPLQK
                     QFEQTRDLIRALVGNLPNQDLSETFEFDALQLPMELPLSLPARIVEQRPDVRAAEAHL
                     HAANAQVGVAIAAMLPQFSITGALGGNADEIPWLFRKGGPFWNLVGNVTQPLFEGGTL
                     LHRKRAADDALKQAAAQYQSSVIAAYQNVADTLHASLSDADALAANVRAENAAKVTYD
                     LTRRQMEVGYVNDLILLNAEASYNQALLARVQAQATRFGDTVALFQALGGGWWNR"
     gene            complement(4399..5928)
                     /locus_tag="VNZ06_14445"
     CDS             complement(4399..5928)
                     /locus_tag="VNZ06_14445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWW21996.1"
                     /translation="MVRSLLLVSSAALAAGCAVGPRYHRPEAPAAAGYAPAPLPQASA
                     SARIHGGEAQRLISDRDIPFEWWELFKSSALNALVERAFKANPTIAAAQAALVQAQEL
                     VYAQRGYFFPAIDANYGFERQKIAGNLTLDNVPGVEGDGHNDLPPVQSLDTYPHTHPF
                     FYDLHTAQLTVGFVPDVFGANWRQVETLAAQRDVQRYTLEATYVTLASNVAAAAIQEA
                     SLRAQIEATREIIAADIKSLQILRDQLRLGFVMRIDVAAQEAALGQAKTLLPPLQRQL
                     EQTRDLMRALVGNLPNDDVPQTFELDALQLPPEMPLSLPARIIEQRPDVRAAEAQLRA
                     ANAQVGVAVAAMLPQFSITGNLGGTADEFAWLFRTGGPFWSIVGSVTQPVFEGGTLLH
                     MKRAANAALKQAAAQYQSTVIAAYQNVADTLHASLSDADALAADLDAENSAKVTYELT
                     RRQLEVGYASTLALLSAESAYQQTLLTRVQAQATRYGDTVALFQALGGGWWNRKVVAA
                     R"
     gene            complement(5931..7451)
                     /locus_tag="VNZ06_14450"
     CDS             complement(5931..7451)
                     /locus_tag="VNZ06_14450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWW21997.1"
                     /translation="MHKLCSRTLLVTCCVLATSCAVAPTYRRPEIPASAGYAPTPLPE
                     VTASAPIQGGDLQRFISGRELPFDWWKLFQSPALNALVAQAFKANPTIPAAQAALAQA
                     QEFVRAQRGFYYPSVAANFQAERVKVAGNNTQSSSLGIQGNGENFGQPLQAAVPLYYQ
                     FYTAGLAVGFVPDAFGANRRQVESLAAQSDAQRFALEATYITLASNVVGAAIQEASLR
                     AQIEATRQIIAADEQCLQILRDQFRLGLAMRIDVAAQEAALAQAQTQLPPLQKQLEQT
                     LDLIRALVGNLPNQDVPQTFELQALQLPPELPVSLPARIIEQRPDVRAAEAQLRAANA
                     QVGAAVAAMLPQFPISGTLGGNAAQIPLMFRGDGPFWTLVGGVSQPVFAGGTLLHTKR
                     AASAALKQAASQYQIAVITAYQNVADSLQASLSDAEALAGAVEAEDAAKVTYDLTRRQ
                     MEAGYVNTLALLYAQTAYNQALLTRIQDQATRYGDTVALFQALGGGWWNRGEVAAR"
     gene            7625..8788
                     /locus_tag="VNZ06_14455"
     CDS             7625..8788
                     /locus_tag="VNZ06_14455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWW21998.1"
                     /translation="MSAGAVLAAVAAGLLAYFSARAETADKPKANAGSKASDSTTVEL
                     SDSQLQLVNLGAVSTYAFPLQREAVGSIDFNEDMTTQVFPPYQGRIAQLFATLGDNVT
                     KGQPLFTIESPDLIQAESTLIAAAGVLDLTSRALDRAKQLHDVQGMADKDLEQAVSDQ
                     QSAEGALRAARNGVAVFGKAQSEVDRIVQTRTIDPFLVVLSPVSGRVTARNAAPGVFV
                     QPGNTPAPYSVADISRVWLNASVMESDMPLVKKGQQIRVSVMAFPERSFEGRISTVGS
                     TVDTQLHRGLVRAEIEDPKHELLPGMFVSFVIVIGDPMSAPAVPLDGVVREGDGSMTV
                     WVTTDGHHFVQRTVKLGLQSGGYDQVIEGVRAGERVVTKGAIILDNMVNGGET"
     gene            8792..>9009
                     /locus_tag="VNZ06_14460"
     CDS             8792..>9009
                     /locus_tag="VNZ06_14460"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW21999.1"
                     /translation="MLKGILAFGLTRRAIIVLGLLVFSCAGLAAFLKLNIEAYPNPAP
                     VILEITAQAAGLSAEEMERYYTVPMEVGL"
BASE COUNT         1632 a         2705 c         2932 g         1740 t
ORIGIN      
        1 gtcttcggat gaccactcac ccggtcgaat ttgacatgta ttacagcgtc tgagcgatat
       61 caatagaaaa ccagggagtt gcaagctatg cgcatgagtc gaatgttcaa atacaaaagc
      121 agaagcctgc aggcggcttt cgccgtgctg gccgccgctc tgctggtgct tgcattgggc
      181 aagccgctga cggcgactgc ggacgaagca cccgcggcag cagcgacggc gcccgcgcct
      241 gcagcggcac ccgcagcgac gcctgctgcg acgacgccgg cggcgccgac cgagccgcag
      301 atcgtgagct tcgacaagct caattcgggc gataccgcct ggatgctcac ctccacagcg
      361 ctggtgttga tgatgaccat tccaggcctc gcattgttct acggtggcat ggtccgcaag
      421 aagaacgtgc tggccacgct gatgcagagc ttcgcgatca cctgtctggt gacgatcctg
      481 tggtgggcga tcggctactc gctggccttc acaccgggtt cgaagtacct cggcgggttc
      541 tcgcgactgt tcatgcacgg tgtgctctac gatcacgatg gcatgaaggt gagcgtgtcg
      601 cacctggccc tgacgatccc tgagaccgtc tacgtgatgt tccaggcgac cttcgccatc
      661 atcacgccgg cgttgatctg cggctcgttt gccgagcgca tgaaattctc ggcgatgatg
      721 tggttcatgg gcatctggct gctggtggtg tactgcccgg tggcgcattg gatgtgggaa
      781 tcaacctcgg gctggtttgc atccacgcag ctggacttcg cgggcggcac ggtggtgcac
      841 atcaatgcgg gtattgccgg ccttgcgacc gcactgatgc tgggtaagcg cgaaggcttc
      901 ggcaaggaga agatggcgcc acacaacctg accctgaccg tcatcggcgc atcgctgctg
      961 tgggtgggct ggttcggctt caatgccgga tcggcagtgg cctcggacgg ccgcgccggt
     1021 atggcgttcg ccgtgacgca catcgccacc gcaaccgcgg cgctggcctg gatgttcacc
     1081 gagtggctga gcaagggcaa gccgagcgtg ctgggcatcg cctcgggtgc ggtggccggt
     1141 ctggtggcga tcacaccggc ttcaggcttc gtcgaccctg gctcctcggt gatcatcggc
     1201 gtagcggcgg gagtgatctg ctacatcgcc tccaccgcag tcaagaaaat gttcggctac
     1261 gacgattcgc tggacgcgtt cggtgtgcac tgcatcggcg gcatcgtcgg cgcgttgttg
     1321 acgggagcat tggtcagcaa gctgatcagc ggcgccgagg gtagtgtgct gctgcagctc
     1381 aagggtgtga gcgccacgct ggtgtggagc ttcgtcatga gcatgatcat cctgaagttg
     1441 atcgatctga cgatcggact gcgcgtgacg actgaacagg agcgcgaagg gttggacatc
     1501 tcgcaacacg gcgagagcat cgaataatca tcgggagaaa tgcgccatga aactaatcac
     1561 agcgattatc aagccgttta agctcgatga ggtgagaacc gccctgacga cgctgggtgt
     1621 ctcgggaatg tcggtgacgg agatcaaggg ctttggccgt cagcgcggtc acaccgagct
     1681 ataccgagga gcggagtaca aggtggattt cgtgcccaag tcacgcgtgg agatcgcggt
     1741 gcgggccgag ctggtcgatc aggtggtgga ggcgatcatc aacgccgcca agaccggcaa
     1801 ggtcggtgac ggcaagatct tcatcagcga tctgcaacgc gtggtacgta tccgtaccgg
     1861 cgaaaccgac gatagcgcgc tctaagcagg cgacgtcggg agaccttgag gctcgggggc
     1921 gggtgtgccg cccccgagcg tccatccaga ccagcccgtt cagcctgcgc ggtgatgctg
     1981 ggccagcaaa tccttcacct gtgcgagctg gcggctggca tccgcgacat tgccgccggc
     2041 acactccgcc gcaacctgct tcagctgcga cttcatccac tgagcctggc cggccgtgaa
     2101 ctccgaaccg tcggatgcga acacacgcat ctgtcccatc ttgtcagggc gcagcgagcc
     2161 gacgatgtgc tcacactcgt gcagtgaggt catgagttgg tcgtcacaca gggtggaacc
     2221 atagacgttc gaggccgtca gcagacccat cgccaagagc gtcaaatatc ggttgttcat
     2281 gatcgcttct ccaaacgtcg ctgtagcgac ggcgcgaaca ttgacaactt cggcggcggc
     2341 tgcaaagaca gcaacgcgtt aagattttcc aaattgcggc gcgcgatagg ttctgcatat
     2401 ttctttgcga catccttatg ctttgcgcac cagcagcacc ggaacagggg cgtggcgtgc
     2461 aacgaactcg gcatcgcttc ccagcacggc tcgggcaaag ccgcgccgac cgtgggtccc
     2521 catgacaatc aggtcggcgt gccaaacttg cgcatcctcg atgatgcctt cgccgatctg
     2581 cgtgctgtgg tgttcgaaca gacgtggctc aacttccagg ccggcagcgc gggccggcgc
     2641 tgccgccgcg ctgagcacgt cctggccatc ttcacgcaat cttcgcatca cgtcgccgct
     2701 cgaagcgcca ccgagcacgg cgctaacata ggcgtagctg accacgtgca gcaggcgcag
     2761 ccgggccttt tgatccacgg ccagattgat cgcctcggtg caggcacgcg atgagctgtc
     2821 agtgccatct atggcgacca ggattctttg atacattgca acccccattg gcaatagcag
     2881 acgactatcg gttccaccaa cctccaccca gcgcctggaa cagagcgaca gtgtcgccaa
     2941 agcgggtggc ctgggcctgc acgcgtgcga gtagggcctg gttgtagctg gcctcggcgt
     3001 tcagcaggat cagatcgttg acgtatccga cctccatctg acgacgagtc agatcgtagg
     3061 tcactttagc ggcattctcc gcacgcacgt ttgcggccaa cgcatcggca tcggacaacg
     3121 aggcgtgcag ggtgtcggca acgttctgat aggcggcgat gacgctgctc tgatactgcg
     3181 ccgcagcctg cttcagcgca tcgtccgcag cgcgctttct atgcagcaac gtaccgcctt
     3241 cgaacaaggg ttgcgtgacg ttgccaacca gattccagaa tggaccgccc ttgcggaaca
     3301 gccacgggat ttcatccgcg ttaccgccca gcgctccggt gattgagaat tgcggcagca
     3361 ttgcggcgat cgcgacccct acctgtgcgt tcgcagcgtg caagtgtgcc tcggcggcgc
     3421 gtacatcggg gcgctgttca acgatcctcg ccggcaggct caacggcagt tccattggca
     3481 gctgcagcgc atcgaactcg aaggtctcgg ataggtcctg gtttggcagg ttgccgacca
     3541 gagcacgtat cagatcgcgt gtctgctcga attgtttctg gagtggaggc aggttcgccc
     3601 gcgcctgtgc cagcgcggtc tcctgcgcgg cgacatcgat gcgcatcgcg aagccgagct
     3661 ggagctggtt gcgcaggatc cgcagggaat tctcatcggc cgcaatgatg tcgcgggtgg
     3721 cgccgatttg tgcgcgcagc gacgcttcct ggatggcagc ggccacgaca ttggaggcca
     3781 gtgtgatgta agttgcctcg agcgcgaagc gctgcgcctg cgtttgcgcc gccagcgatt
     3841 ccatctgtcg ccagttggcg ccgaacagat cgggcacgaa tcccacggtg agctgcgcgg
     3901 tgtgatagtt gtagatgagg ggtgccgtgt gaggactgtt ctgcaggtca agcaggggtg
     3961 ggttgaggtt gtgtccgtcg ccctgcgtgc cgggagagtc gtcgacggtg aagttaccgg
     4021 ctatcttcgt gcgcgcgaag ttgtagttcg cgcccaatgt cggaaagaag tagccccgct
     4081 gcgcgtagac cagctcttgg gcttgaatca gcgccgcctg agccgccgcg atgcttggat
     4141 tggccttgaa tgcccgctcc accagtgagt tgagcgccgg tgattggaac tgctcccacc
     4201 acttaaaggg tatgtcgcgg ccgctgatca ggcgctgtgt ctctccggca tgtaccggtg
     4261 ccgaagcgct tgattgcggc aggggctccg acgcgtagcc gggattcgca ggggctccgg
     4321 gtctatggta gcgagggccg accgcgcagc ttgcggcgag tgtcgcggac gcaacgagca
     4381 gcgcggcgcg gacgtcgctc atctcgccgc gactactttt cgattccacc agccgccgcc
     4441 gagcgcctga aacagcgcga cggtgtcgcc atagcgcgtc gcctgggctt gtacgcgcgt
     4501 gagcaaggtc tgttggtagg cgctctcggc gctcaatagc gcaagagtgc tcgcgtagcc
     4561 aacttccagt tggcggcgcg taagctcgta agtgaccttt gcggaatttt ccgcatcgag
     4621 gtcggcggcg agcgcatcgg cgtcggacag cgacgcatgc agggtatcgg cgacgttctg
     4681 gtaggctgca atcaccgtgc tttggtattg cgccgcggcc tgtttcaatg ccgcgttcgc
     4741 ggcgcgcttc atatgcagca gcgtaccgcc ttcgaagaca ggttgtgtga cactgccgac
     4801 gatgctccag aacgggccgc ccgtacgaaa caaccaggcg aattcgtccg cggtgccgcc
     4861 aagatttccg gtgattgaaa attgcggtaa catggcggcg accgccacgc ccacctgagc
     4921 gttcgcggcg cgcagctgcg cctcggcggc ccgcacgtcc ggacgctgtt cgatgatcct
     4981 cgccggcagg ctgagcggca tctccggcgg caactgtaac gcatcgagct cgaaggtctg
     5041 cggcacgtcg tcgtttggca ggttgccgac caatgctcgc atcagatcac gcgtctgctc
     5101 gagctgcctc tgtaatggcg ggagcagagt ctttgcttgc cccagagcgg cttcctgagc
     5161 ggcgacatcg atgcgcatca caaatcccag acgcagttga tcgcgcagga tctgcaggga
     5221 ttttatatct gccgcaatga tctcacgggt ggcttcaatc tgtgcccgca gcgacgcttc
     5281 ctggatggcg gcggcggcga cattggaggc cagcgtgacg taagtggcct ccagcgtgta
     5341 gcgctgcacg tctctttggg ctgccagggt ctccacttgc cgccaattgg cgccaaagac
     5401 gtccggcacg aatccgacag tcagctgcgc agtgtgcaag tcatagaaga aggggtgggt
     5461 gtgaggatac gtgtccaggc tctgtacggg gggcaggtcg ttgtggccgt caccttctac
     5521 gccgggaaca ttgtctagcg tgaggttgcc agctattttc tggcgttcga agccatagtt
     5581 cgcgtcaatc gctggaaaga agtaaccgcg ctgcgcgtag accagctctt gggcttgaac
     5641 cagcgccgcc tgtgccgccg cgatagtggg attggccttg aacgcccgct cgaccaacgc
     5701 attgagggcc gatgacttga acagctccca ccactcgaag ggaatatcgc gatcgctgat
     5761 caggcgctgc gcttcgccgc catgaattcg cgccgaggcg ctcgcctgcg gcaacggcgc
     5821 cggcgcgtag ccagcggcgg caggtgcttc cggtctgtgg taacgcgggc cgacggcgca
     5881 gcctgcggcg agtgccgcgg agctgacaag cagaagtgag cgcaccatcc tcatcttgcg
     5941 gccacttcgc ctcggttcca ccagccgccg cccagtgcct ggaacaacgc aacagtgtcg
     6001 ccatagcgtg tcgcctggtc ctgtatgcgc gtgagcagcg cctggttgta cgctgtctgg
     6061 gcgtataaca gagccagagt gttcacatac cccgcttcca tttgccggcg tgtcaggtcg
     6121 taggtcactt tggcggcatc ctcggcctcc accgcgccag cgagcgcctc ggcatccgat
     6181 agcgatgcct gcaagctatc cgctacattc tggtaggcgg tgatcacggc gatctgatac
     6241 tggctcgcag cctgcttcag cgctgcgctg gcggcacgct tggtatgcag cagcgtgccg
     6301 cctgcgaata ccggctggct aacgccgccg acaagtgtcc agaacgggcc gtcgccgcgg
     6361 aacatcaagg ggatttgggc cgcgttgccg cccagagttc cgctgattgg gaactgcggc
     6421 agcatcgccg cgacggcagc gcccacttgt gcgttcgcgg cgcgcagttg cgcctcggcg
     6481 gcccgcacgt ccggacgttg ttcgatgatc ctggccggta agctcaccgg cagttccggc
     6541 ggcagttgca gtgcctgcag ctcgaatgtc tggggcacat cctggttcgg caagttgccg
     6601 accagagcac gaatcagatc gagcgtctgc tcgagctgct tctgcagggg gggcagctgg
     6661 gtctgcgcct gtgcgagggc cgcctcctgg gccgcgacgt caatgcgcat cgcgaggccc
     6721 aaacgaaact ggtcacgaag aatctgcaga cactgctcat cggctgcgat gatctgccgg
     6781 gtcgcctcaa tttgcgcccg cagcgacgct tcctggatgg cagcgccgac aacattggag
     6841 gccagcgtga tgtaagtcgc ctccaacgcg aagcgttgcg cgtcgctctg cgccgccagc
     6901 gactcgacct ggcgccgatt ggcgccgaat gcgtcgggta cgaatccaac ggcgagcccc
     6961 gcggtgtaaa actggtagta gagaggcacg gccgcctgca gcggttgccc aaagttctcg
     7021 ccgttgccct ggatgccgag cgaggaactt tgagtgttgt tgccggctac cttgacacgc
     7081 tcggcctgga aattggccgc aacgctcgga tagtagaagc cgcgttgcgc acgcacgaac
     7141 tcctgcgcct gagccagcgc ggcctgtgcc gcggggatgg ttgggttggc tttgaacgcc
     7201 tgtgcgacca gcgcgttgag cgccggagac tggaatagtt tccaccagtc gaacggcagc
     7261 tcgcggccac tgatgaaacg ctgcaggtcg ccaccctgaa tcggtgcgga cgcagtgact
     7321 tctggcaagg gcgtcggcgc gtaacccgcg ctcgccggta tctctggtcg gcggtaggtc
     7381 ggcgcaaccg cgcagctcgt ggcaagcacg cagcacgtga cgagcaaagt tctgctgcag
     7441 agcttgtgca tatcaggagc gatactgcgt tggtacgtcg caggccgaca actcggaaat
     7501 cacacagttg tcagtcgtag gagttgccgc cattgtccgc ccggatcctg aagcgggagt
     7561 tgcgatggac aatccgtggt tttcggttcg gcgtgcttgg cagttaaggc cctttcggcg
     7621 ctccgtgagt gcaggagcag tgctggcagc cgtcgccgcc ggactgctgg cctacttcag
     7681 cgctcgagcc gaaacggcag acaagcccaa ggccaacgca ggctccaagg catccgattc
     7741 cacgaccgta gagctgtcgg atagtcagct gcagctggtg aatctcggcg cggtcagcac
     7801 ctacgccttt ccgctgcagc gcgaggcggt cggcagcatc gacttcaacg aagacatgac
     7861 tacgcaggtg ttcccaccct accaggggcg catcgcgcaa ttgtttgcca cgctcggcga
     7921 caacgtcacg aagggtcagc cgctcttcac gatcgagagt cctgatctga ttcaggccga
     7981 gtcgacccta attgctgcag cgggagtctt ggacctgaca agtagggcgc tcgatcgcgc
     8041 caagcagctt cacgacgtgc agggcatggc ggacaaggat cttgagcagg ctgtctcaga
     8101 tcaacaaagc gcggaagggg ctctgcgggc ggcgcgcaat ggagtggcgg ttttcggtaa
     8161 ggctcagtct gaggtcgacc gcatcgtgca gactcgcacg atcgatccgt ttctggtcgt
     8221 gctcagtccg gtcagcgggc gggtcacagc gcgcaatgca gcccccggcg tcttcgtgca
     8281 acccggaaac accccggcgc cctattcagt cgccgatatc tctcgtgtgt ggttgaacgc
     8341 cagcgtcatg gaaagcgaca tgccactggt aaaaaagggt cagcagattc gagtctcggt
     8401 catggcattt ccggagcgca gcttcgaggg ccgcatctca accgtcggat ccacggtgga
     8461 cacgcagctg caccgcgggc tggtgcgcgc agagatcgag gacccgaaac acgagctgct
     8521 gccgggcatg ttcgtatcgt tcgtcattgt catcggcgat ccgatgagtg cgcccgcagt
     8581 accactcgac ggcgtggtgc gggagggcga tggatcgatg actgtctggg tgaccaccga
     8641 cggacatcac ttcgtacagc gcacggtgaa actgggtctt cagagcggcg gctacgatca
     8701 ggtgatcgaa ggcgtgcgtg cgggtgagcg tgtcgtgacc aagggcgcaa tcattctcga
     8761 caacatggtc aatggcgggg agacctgacc ggtgttgaag ggcatccttg cgttcggcct
     8821 gacgcgccgg gcgatcatcg tgctcgggct gctggtattc agctgcgccg gccttgctgc
     8881 gttcctcaag ctcaacatcg aggcatatcc aaacccggcg ccagtgatcc tcgaaatcac
     8941 tgcgcaggcg gctggtctct cggccgagga gatggagcgt tactacacgg tgccgatgga
     9001 ggtggggct
//
LOCUS       DATMRM010000475        11361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2753194, whole genome shotgun sequence.
ACCESSION   DATMRM010000475 DATMRM010000000
VERSION     DATMRM010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11361
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2753194"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /gene="gcvPA"
                     /locus_tag="VNZ06_14465"
     CDS             complement(<1..916)
                     /gene="gcvPA"
                     /locus_tag="VNZ06_14465"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HWW22000.1"
                     /translation="MPFIPHTDDDVREMLAVIGVPSIETLFEEIPAGLRVQSLAEVPE
                     GLSELEVMQLMTERAHADGRPKCFIGAGAYEHHIPAAVWSTVTRGEFYSAYTPYQAEA
                     SQGTLQTIYEYQTMMASLTGMQVSNASLYDGATAVAEASLMAVRANRRSKSARILLPR
                     SLHPHYRDVTLASASNQGLKFEELPYDRATGQLAPGALSGLDGQDITALVIQQPNFLG
                     VMEEVDALTDWAHAHGALVVAVVNPISLALLKPPGQWGAKGADIAVGDGQPLGCPLSS
                     GGPYFGFMTTRMEHVRQMPGRIVGRTVDL"
     gene            complement(938..1327)
                     /gene="gcvH"
                     /locus_tag="VNZ06_14470"
     CDS             complement(938..1327)
                     /gene="gcvH"
                     /locus_tag="VNZ06_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HWW22001.1"
                     /translation="MSNIPTDLKYARTHEWLRTLSDGNVEVGISDHAQAALGDLVFVQ
                     LPDVGRAVAAGDACAVVESVKAASDLYAPVAGHVVAINEALSKEPELINQDPYGRGWV
                     MRLKPDGATAGLLSAADYQKVADESEG"
     gene            complement(1340..2428)
                     /gene="gcvT"
                     /locus_tag="VNZ06_14475"
     CDS             complement(1340..2428)
                     /gene="gcvT"
                     /locus_tag="VNZ06_14475"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HWW22002.1"
                     /translation="MGRHTCLYDLHVALGARMVDFGGWDMPVAYGSQIEEHQAVRRSA
                     GVFDVSHMCIVDVTGARARDLLSRLLANDIGRLTTLGKALYTCMLNDSGGVIDDLIVY
                     RMEGGDYRLVVNAGTRDKDMAWIGAHAASLDVSLLERRDLAMLAIQGPQARERTASLL
                     SYDGSRTVLALETFFAARLGEWFVARTGYTGEDGFEVMLPASEAGSFWQRLNGAGVRC
                     CGLGARDTLRLEAGMNLYGNDMDEQTHPLESGLGWTVAFEPSARDFIGRAALEAIRIR
                     GSDNKLVGLVLEERTVLRSHQKVKVPGVGDGEVTSGTFSPTLGRSIGLARVPSATQAT
                     VEVEVRGRFLPARVVRPPFVRHGKVLVQ"
     gene            2587..3825
                     /gene="ispG"
                     /locus_tag="VNZ06_14480"
                     /gene_synonym="gcpE"
     CDS             2587..3825
                     /gene="ispG"
                     /locus_tag="VNZ06_14480"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWW22003.1"
                     /translation="MESKRHLSQPVSIAGVTIGGRAPIVVQSMTNTDTADVEATARQV
                     QQLAAAGSELVRITVNAADAAAAVPHIRDRLRQLRCNVPLIGDFHFNGHKLLQEHPAC
                     AEALDKYRINPGNVGRGSKRDSQFAEMIEIAIRNQKPVRIGVNWGSLDQDLLVRLMDE
                     NNRLPEPVSAAEVTREAIVRSAIESAERAVELGMPCNRIILSAKVSGVQDLIAVYTQL
                     ASRCEFALHLGLTEAGMGSKGIVASSAAMGILLQQGIGDTIRVSLTPEPGGDRTREVI
                     VAQELLQTMGLRSFTPLVTACPGCGRTTSTYFQTLADRIQRHLRERMPQWRDTYRGVE
                     SMTVAVMGCVVNGPGESKHANIGISLPGTGERPVAPVYEDGEKTVTLKGDRIAEEFQE
                     LVERYVERTYARRSVAEVQA"
     gene            3839..4177
                     /locus_tag="VNZ06_14485"
     CDS             3839..4177
                     /locus_tag="VNZ06_14485"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002019.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HWW22004.1"
                     /translation="MSDFAERHCQPCEGKVAAYTTAQAGAALTQLAPGWQLIEDGRAI
                     KREFKFRDFFRTMSFVNALAHIANIEDHHPDLELGYNYCRVRFSTHALGGLSDNDFIC
                     AAKIDRLPLH"
     gene            complement(4202..4795)
                     /locus_tag="VNZ06_14490"
     CDS             complement(4202..4795)
                     /locus_tag="VNZ06_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22005.1"
                     /translation="MNTTPWRRALAFYWAFAWRACLICLGLIIAFYLVAAPIESATRM
                     PPRAAALFDLGALLLLLAAGACLALQWCSRARFGAYRLRVEPRSAIGLSFAQAARVAW
                     AQFWRSAVVTAPIYFIVRWLLAPLILWLLDPTTPWQHLLDRQLIYLPFTAAIGFWAMR
                     EAVELKYRGFRLQWMPEPLRDDPVADAQRARPAAALQ"
     gene            complement(4911..6140)
                     /locus_tag="VNZ06_14495"
     CDS             complement(4911..6140)
                     /locus_tag="VNZ06_14495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWW22006.1"
                     /translation="MRHDFDVVVVGGGLAGACAAALLARHAGLAAERVGLLSEESPVA
                     RVAGDPAELRVVAISRASERVLRAAGAWSRLDPERLCAYERVRVWHETSSATGPSALR
                     FDAADIAEPNLGFIAENSALVRACLDSFTAAGGSRIDSMLERASFDAAPVILQAAGGR
                     ELSARLVVGADGSQSVVRASAGLSARTLDYRQLAIVATVRTSQSHENTAWQRFLRTGP
                     LALLPLFDGHSSIVWSVDQGPARELLDLSEQAFNERLTAASDEVLGSARVVSQRISFP
                     LRSLAARSYVAPRCALVGDAAHVVHPLAGQGANLGLLDAAALCEVIADAIAEREDPGA
                     LRALRRYEQQRRTHNLMMDSAMSAFRAGFAASVGPAALLVNTALGAVNRSGALKRLFA
                     RQALGTAGALPKYARNR"
     gene            complement(6144..7367)
                     /gene="ubiH"
                     /locus_tag="VNZ06_14500"
                     /gene_synonym="visB"
     CDS             complement(6144..7367)
                     /gene="ubiH"
                     /locus_tag="VNZ06_14500"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF004356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HWW22007.1"
                     /translation="MNAPSLDRYDVAIVGGGMVGASLALALAGTQRRVLLIEAVAADS
                     SQQPSFDDRTTALGNGARRILQTLGVWADIEPRATPITQIHVSDAGRFGFARLRASEH
                     ELHAFGCTVSNRHIGAALWRALQNQQRIEIRSPARVGQVSLDSESASLTVQAASGTAS
                     TVQARLVVAADGAHSMVKQAAGIASDNVSYDQLAVVANVRTDRRACGIAYERFTDSGP
                     LAVLPLYDGAYTVVWTLGPQIAEAMRQCGADEFCRELQNGFGWRAGRFMQVGQRGSYP
                     LALVTAQASVGKRVALIGNAAQALHPVAAQGFNLGLRDAAVLAELIVSAEDPGADSVL
                     AQFAARRSADRAGMIAFTDRLVKLFSSRRPGAAALRDLGLLLFDINPIAKQALSRISW
                     GFGGALPRLSRGLPL"
     gene            complement(7364..8686)
                     /gene="pepP"
                     /locus_tag="VNZ06_14505"
     CDS             complement(7364..8686)
                     /gene="pepP"
                     /locus_tag="VNZ06_14505"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF008131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HWW22008.1"
                     /translation="MPRSRSALDEFERRRTQLMRLIGEEGIAILPAAPVRQRNNDVDY
                     NYRQDSDFYYLTGFSEPDAVAVLIPGRPAAQYILFIRERDPDREIWDGKRAGPAGAVE
                     EYRADDAFPICDIDEILPGLLENRSRVFYTMGLNQEFDHHVVSWVNTLRAQARTGRHS
                     PQEFVALDHVLHDMRLFKSRAEVSLLRRSGQIAAGAHVRAMRFCRPGRMEYEVMAELV
                     HEFNRHGADISYHPIIGAGPNSCVLHYRDNNARLNDGDLLLVDAGCEFGYYASDITRT
                     YPVSGRYSSEQRAIYEIVLDAQQAAIAKVKPGNHWNEPHDAAVRAITQGLKRVGLLKG
                     SLPGLIRNGAYRQFFMHRTGHWLGMDVHDVGDYKVGDEWRVFEPGMVLTVEPGIYIGA
                     GARGVPRKFWNVGVRIEDDVVVTRSGSEVLTDGAPKDPDAIEALMAAA"
     gene            complement(8695..9252)
                     /locus_tag="VNZ06_14510"
     CDS             complement(8695..9252)
                     /locus_tag="VNZ06_14510"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HWW22009.1"
                     /translation="MSIQHDYAEIQRSLAHSHALADPAEAHGQLAGALCALVPYRMED
                     WLAEILPEDSLSTAADPVLQTLYQATVAALSSGEMEFDILIPADDRPIEERTQALTLW
                     CTGFLYGLGTSAADPQRLPGELGEIVRDLTEITRADVDAGDTQEANESALAELVEFVR
                     VGVQLVFDELSPLRERHTPPDHVLH"
     gene            9348..9581
                     /locus_tag="VNZ06_14515"
     CDS             9348..9581
                     /locus_tag="VNZ06_14515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HWW22010.1"
                     /translation="MAEAKIPSAIDLELKRLERRVDELLTTVQQLKEENRALRQRQDT
                     LAAEKTSLLNRNEQVRTRVEAMIGRLKAMEHSA"
     gene            9578..9898
                     /locus_tag="VNZ06_14520"
     CDS             9578..9898
                     /locus_tag="VNZ06_14520"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HWW22011.1"
                     /translation="MSEREAVRPLGVRILEKEYIVACGPDERSELIDSAEFLNKRMKE
                     IRDSGKIIGLDRIAVMAALNLAHEVIKMRARDTGLEQEAGHRIKLLRERVESALEKGQ
                     QLEL"
     gene            9963..10153
                     /gene="ssrS"
                     /locus_tag="VNZ06_14525"
     ncRNA           9963..10153
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VNZ06_14525"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            10147..10773
                     /locus_tag="VNZ06_14530"
     CDS             10147..10773
                     /locus_tag="VNZ06_14530"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HWW22012.1"
                     /translation="MAPLNSPDNDIAAGERLELRRTLRRRRADLSAQQRATAARAITS
                     HVAATRWLQGARPIALYLSVGSEVDTGPLSELAQRRRCPVYLPRITDYRTHRMQFVLL
                     SAAQNERNRHGIPEPGGRRSICPGALSVVFLPVLGFDARGTRLGSGAGYYDRVFSFRR
                     HRLSWHRPLLVGVSYRCQELPCIEPQRHDVPLDALVTEDGVRYFRQGG"
     gene            10779..11243
                     /locus_tag="VNZ06_14535"
     CDS             10779..11243
                     /locus_tag="VNZ06_14535"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HWW22013.1"
                     /translation="MNHWLLKTEPTTFGVDHLEAAARKTTGWDGVRNYQARNYIRDQM
                     KKGDEGFLYHSSCAIPGVVAIVTVVKAPYPDETAFKRGDPHFDPDSDPAAPRWYSMDI
                     KLTRRLKRVITLEELKAHAKKQLSGMILLRPGNRLSVSPVEDAHWRFVLSLE"
BASE COUNT         2155 a         3776 c         3494 g         1936 t
ORIGIN      
        1 ccagatcgac cgtgcgtccg acaatgcgac ccggcatctg gcgcacatgc tccatgcgcg
       61 tggtcatgaa tccaaagtag ggcccgcccg acgacagcgg acagcccaac ggttgtccgt
      121 cgcccaccgc aatatcggcg cccttggcgc cccactggcc cggcggcttc agcagcgcga
      181 gtgaaatcgg gttgaccacc gcgacgacca gcgcgccatg ggcatgcgcc cagtcggtca
      241 gcgcatcaac ctcctccatc acgcccagga aattcggctg ctggatcacc agcgccgtga
      301 tgtcctggcc atccaagccg ctcagcgcgc ccggcgcgag ctgcccggtc gcgcggtcgt
      361 agggtagctc ctcgaatttc agcccctggt tcgaggcgct ggcgagcgtg acgtcgcgat
      421 agtgcgggtg cagcgagcgc ggcaacagga tgcgggcgga cttggagcgc cggttggcgc
      481 gcaccgccat cagactggct tcggccacgg cggtcgcgcc gtcgtacagc gaggcgttag
      541 acacctgcat gcccgtcagg ctcgccatca tggtctggta ctcatagatg gtctgcagcg
      601 tcccctggct ggcttccgcc tgatagggcg tataggcgct gtagaactca ccgcgcgtca
      661 cggtcgacca gaccgctgcc ggaatgtgat gctcataggc accggcgccg atgaaacatt
      721 tcggccgacc atcggcgtgc gcgcgctcgg tcatcagctg catcacttcc agttcgctga
      781 gaccttccgg gacctcggcc agcgattgca cgcgcagccc ggcgggaatc tcctcgaaca
      841 gcgtctcaat gctcggcacg ccaatgaccg cgagcatctc gcgcacgtcg tcgtcggtat
      901 gaggaatgaa cggcatcggc ttgagctcct tcgcgcctca gccttcgctt tcgtcagcga
      961 ccttctgata gtccgcggcc gacagcagcc ccgctgtcgc accgtcgggc ttcaggcgca
     1021 tcacccagcc gcgaccatag ggatcctgat tgatgagttc gggctccttc gatagcgcct
     1081 cgttgatggc aacgacatgg cctgccaccg gcgcgtagag gtcggaggcc gctttcaccg
     1141 attcgaccac cgcgcaggca tctccggcag cgactgcgcg cccgacatcc ggcagctgca
     1201 cgaacaccag atcgcccagc gccgcctgcg cgtgatcgct gatgcccact tcaacgttcc
     1261 cgtcgctcag tgttctgagc cactcgtggg tgcgtgcgta cttgaggtcg gtcggaatgt
     1321 tactcatcga caagctcctt cactgaacca gtaccttccc atggcgcaca aacggcggtc
     1381 gtaccacacg tgccggcaga aaacgtccac gcacctcgac ttcgacggtt gcctgggtgg
     1441 cggacggtac ccgcgccagt ccgatcgaac gcccgagcgt cggtgagaag gtgccgctgg
     1501 tgacttcgcc gtctcccacg ccaggcacct tcaccttctg gtggctgcgc agcaccgtac
     1561 gctcttccag taccaggccc accagcttgt tgtcgctgcc acgtattcgg atcgcctcga
     1621 gcgctgcgcg cccgatgaaa tcgcgcgcgc tgggttcgaa ggctacggtc cagcccaggc
     1681 ccgactccag cggatgggtc tgctcatcca tgtcgttgcc atagagattc ataccggctt
     1741 ccagccgcag cgtatcgcgc gcgcccaaac cgcagcagcg cacgcccgcg ccgttcagtc
     1801 gttgccagaa actgcccgcc tcgctggctg gcagcattac ttcgaatcca tcctcgccgg
     1861 tgtagcccgt gcgcgcgaca aaccattcgc caagacgcgc ggcaaagaag gtttcgagcg
     1921 ccaggacggt gcgtgagccg tcataggaca acagcgatgc ggtgcgttcg cgcgcctggg
     1981 ggccctgtat cgccaacatc gccaggtccc gtcgctccag gaggctgaca tcgaggctgg
     2041 cggcatgcgc gccgatccag gccatatcct tgtcacgcgt tccggcgttg acgaccagcc
     2101 ggtagtcccc gccctccatt cggtagacga tcagatcgtc gatgaccccg ccgctgtcgt
     2161 tgagcatgca ggtgtagaga gctttgccga gcgtcgtcag ccgaccgatg tcattggcga
     2221 gcaaccggga cagcaggtcc cgggctcgcg cacccgtgac gtcgacgata cacatgtgtg
     2281 aaacgtcgaa cacgccagcc gagcggcgca ccgcctggtg ctcctcgatc tgcgaaccat
     2341 aggcgaccgg catatcccag ccgccgaagt caaccatgcg tgccccaagg gctacatgaa
     2401 ggtcatataa gcaggtgtgc ctgcccacac gcgtctccaa aacgtccgtt cctgcccgcg
     2461 ctggcgcgag gtatgccggc ttgagcgggc aactgcggca atgggatgac aggtgttatc
     2521 gacgggtagc tgaatcataa cagtgcggac tgcttcgcca ctacgatttg ccactacaat
     2581 tggtgcatgg aatccaagcg acacctcagc cagccggtca gcatcgctgg cgtcacgatc
     2641 ggcggcaggg cgccgatcgt cgtacagtcg atgaccaaca ccgacaccgc cgatgtcgaa
     2701 gccaccgccc gacaggtcca gcagctcgct gccgccggtt cggagctggt ccgcattacg
     2761 gtcaatgcag ccgatgcggc cgccgcagtg cctcacattc gcgaccggct gcggcagttg
     2821 cgctgcaacg tgccgttgat cggggacttt cactttaacg gccacaagct gctccaggag
     2881 cacccggcct gcgccgaagc gctcgacaaa taccgtatca atcccggtaa cgtcggtcgc
     2941 ggcagcaagc gtgattcgca gtttgccgag atgatcgaga tcgccatccg caaccagaag
     3001 ccggtgcgga tcggggtcaa ctggggcagc ctcgatcagg atctgctcgt gcgcctgatg
     3061 gacgagaaca accgattgcc cgagcccgtc tccgccgccg aagtcacgcg cgaagccatc
     3121 gtgcgttcgg cgatcgagtc ggccgaacgc gcggttgagc tcggaatgcc atgcaaccgg
     3181 atcatcctgt ccgccaaggt cagcggcgtt caggatctga tcgcggtcta cacgcagttg
     3241 gcatcgcgct gcgaattcgc gctacatctg ggattgacgg aagccggcat ggggtccaag
     3301 ggcatcgtcg cctcttccgc ggcgatgggg attttgttac agcagggaat cggcgacacg
     3361 atccgtgtct cgctgactcc ggaacctggc ggcgatcgca cgcgggaagt catcgtcgct
     3421 caggagttgt tgcaaacaat gggtctgcgc tcctttacgc ctctggtgac ggcctgcccc
     3481 ggctgtggtc gcaccaccag cacctatttc cagacactgg ccgaccgcat tcagcgccat
     3541 ctgcgtgagc gcatgccaca gtggcgcgac acctaccgcg gcgtcgaaag catgacggtt
     3601 gcggtgatgg gctgcgtggt caatggtcct ggcgagagca aacacgccaa tatcggcatc
     3661 agcctgcccg ggaccgggga gcggccggtc gctccggtct acgaggatgg cgagaagacc
     3721 gtcacgctca aaggcgatcg gatcgccgag gaattccagg aactggtcga gcgttacgtc
     3781 gagcgcacct acgcgcgtcg cagcgttgcc gaagtgcaag cctaggggcg cacccgcgat
     3841 gagtgacttc gccgagcgtc actgccagcc gtgcgagggc aaggttgccg cctatacaac
     3901 ggcacaggcc ggcgccgctt tgacgcagct tgcacccggg tggcagctca tcgaggatgg
     3961 cagagccatc aagcgtgaat tcaaattccg ggacttcttc cgcacgatga gcttcgtcaa
     4021 cgcactcgcc cacatcgcga atatcgaaga ccatcatccg gacctggagc tcggctacaa
     4081 ctactgtcgc gtacgcttct cgacccacgc gctcggtggc ctgagtgata acgacttcat
     4141 ctgcgcggcg aagatcgatc gcctgccgct gcactagtcg cactcaccgc tccctgcagc
     4201 tttactgcag cgctgcggcg ggcctcgcac gctgcgcgtc agccaccgga tcgtccctga
     4261 gtggctccgg catccactgc agacggaagc cgcggtactt gagctcgact gcctcgcgca
     4321 tggcccagaa gccaatcgcg gccgtgaacg gcagatagat gagctgacga tccagcagat
     4381 gctgccacgg cgttgtcgga tccagcagcc acaagatcag cggcgccaat agccatcgca
     4441 cgatgaaata gatcggcgct gtgaccacgg cgctgcgcca gaactgtgcc caggccaccc
     4501 gcgccgcctg cgcgaagctc agaccgatgg cgcttcgcgg ctcgacccgc agccgatacg
     4561 caccgaagcg cgcacgtgag caccactgca gtgccaggca cgcgccagcg gccagcagca
     4621 gcagcagcgc gccaagatcg aacagcgccg ccgcccgtgg tggcatccgc gtcgcggact
     4681 cgatcggagc ggccaccaga tagaatgcga tgatcagtcc aagacagatc agacaggcgc
     4741 gccacgcaaa cgcccaatag aacgcaagcg cgcgacgcca cggagtggtg ttcatgaaga
     4801 tagactcgta gcccctgttc ctgccggcga ttgtttgccc gtctcgccgt tgccagcatc
     4861 atatgccgag gtggccgcaa tcccgcaatc gaacggatta tgcaaaatcg tcagcggttg
     4921 cgagcgtact tcggcaatgc gccggcggtt cccaatgcct gtcgcgcgaa cagacgcttg
     4981 agagcgccgc tgcgattgac cgcgccgagc gcggtgttga ccagcagtgc ggccggaccg
     5041 acggaggctg cgaatccggc gcgaaatgcg ctcatcgcgc tgtccatcat cagattgtgg
     5101 gtgcggcgtt gctgttcgta gcgacgcagc gcccgcaggg cacccgggtc ctcacgctcg
     5161 gcgatggcgt cggcaatcac ttcgcacaat gcagccgcat ccagcaggcc gagattcgca
     5221 ccctgaccgg ccagcggatg caccacgtgc gcggcatccc cgacgagcgc gcagcgcggc
     5281 gcgacatacg agcgggccgc caggcttcgc agcggaaagc tgatgcgttg actgaccacc
     5341 ctcgcgctgc ctagcacttc atcgctcgcc gcagtcagtc gctcattgaa cgcctgttcg
     5401 gaaagatcga gcagttcgcg cgccggaccc tggtccacag accagacaat cgagctgtgg
     5461 ccgtcgaaca gcggcagcaa cgccagcggc ccggtccgca gaaaccgttg ccacgcggtg
     5521 ttttcatgcg actgggaggt acgtacggtg gcgacgatcg caagctgccg atagtcgagc
     5581 gtacgcgcgc tcaggcccgc cgacgcacgt acgaccgact gcgaaccatc ggcccccacc
     5641 accaggcgcg cgctcagctc acggccgccg gcagcctgca atatgaccgg tgcggcatca
     5701 aagctcgccc gctcaagcat cgaatcgatc cggctgccgc ctgcggccgt gaagctgtcg
     5761 agacaggcac gcaccagcgc gctgttctcg gcaatgaagc cgagattggg ctctgcgata
     5821 tccgcagcat cgaagcgcag cgcactcggg ccggtcgcgg aactggtttc gtgccacacg
     5881 cgcacgcgct catacgcgca caggcgctcg ggatcgagcc gcgaccacgc ccctgcggcg
     5941 cgcaataccc gctcgctcgc ccgcgaaatc gccaccacgc gcagctcggc cggatccccg
     6001 gcaacacgag cgaccggtga ctcttccgac agcagcccca cgcgctcagc tgccaggccg
     6061 gcgtgacgcg cgagcagcgc cgcggcgcag gcacccgcca aacccccgcc gaccaccacc
     6121 acatcgaaat cgtgacgcat gacctaaagc ggcagaccgc gcgacaggcg cggcagcgcg
     6181 ccgccgaaac cccagctgat gcgcgacagc gcctgcttgg cgatcgggtt gatgtcaaac
     6241 aacagcaagc ccaggtcgcg gagcgccgcg gcaccgggtc gccggctgct gaacaatttc
     6301 accagtcggt cagtgaacgc aatcatgccg gcgcgatcag cactgcgtcg ggcggcgaac
     6361 tgcgccagta cgctgtcagc gccgggatcc tcggccgaca cgatcagctc cgccagcacc
     6421 gccgcatcgc gcagtcccag attgaagccc tgggccgcca ccggatgcag cgcctgggcc
     6481 gcgttgccga tcagcgccac acgcttgcca acgctggcct gcgccgtgac cagcgccagc
     6541 ggatagctac cacgctgacc tacctgcatg aaccggccgg cgcgccagcc gaagccgttc
     6601 tgcagctcgc ggcaaaattc atcagcgccg cactgtcgca tcgcctcagc gatctgcgga
     6661 cccagcgtcc agaccacggt ataggccccg tcgtagagcg gcagcaccgc caacgggccg
     6721 ctgtcagtaa accgctcata ggcgatgccg caggcgcgac ggtcggtgcg aacattggcc
     6781 accacggcga gctgatcgta tgacacgttg tcggaggcga tcccggcagc ctgcttgacc
     6841 atggaatggg ctccgtccgc ggcaacgacc aaccgcgcct ggaccgtcga ggcggtaccg
     6901 ctcgcagctt ggacggtcag gcttgcagac tcgctgtcga gcgatacctg tccgacgcgc
     6961 gccggactgc gaatctcgat gcgctgctgg ttctgcagcg cgcgccacag cgccgcccca
     7021 atgtggcggt tactgacggt gcagccaaac gcgtgcagtt catgttcact cgcgcgcagc
     7081 cgcgcgaagc cgaagcggcc cgcgtccgaa acgtggatct gcgtgatcgg tgtcgcacgc
     7141 ggttcgatat cagcccagac acccagggtt tgcagaattc ggcgggcgcc attgccaagc
     7201 gcagtggttc ggtcgtcgaa actgggctgc tggctgctgt cggcggcgac ggcttcaatc
     7261 agcagtacgc ggcgctgcgt cccggccagt gccagagcca ggctcgcgcc gaccatgccg
     7321 ccgcccacaa tcgctacatc gtagcgatcc agcgacggcg cgttcatgcc gccgccatca
     7381 aggcttcgat cgcgtcggga tccttcggag caccatcggt cagcacttcg ctgccgctgc
     7441 gcgtgacgac gacgtcatct tcgatgcgaa cgcccacgtt ccagaatttc ctgggaacgc
     7501 cacgcgcgcc ggcaccgata tagatgcccg gttcaacggt caggaccatt ccgggttcga
     7561 acacgcgcca ctcgtcgccc accttgtagt cgccgacatc gtgcacgtcc atccccagcc
     7621 aatgcccggt ccggtgcatg aagaactgac gatacgcccc gttacggatc aggccgggca
     7681 gtgagccctt cagcaggccg acacgcttga ggccctgagt gatcgcgcgt accgcggcat
     7741 cgtggggttc gttccagtga ttgccgggtt tgaccttggc gatcgccgcc tgctgagcgt
     7801 cgagcacgat ttcgtagatc gcgcgttgct cgctgctata gcggccactg accggatagg
     7861 tgcgtgtgat gtcggacgcg tagtaaccga attcgcagcc ggcatccacc agcaacagat
     7921 caccgtcatt caagcgggcg ttgttgtcgc gatagtgcag gacgcagcta ttgggtccgg
     7981 cgccgatgat cgggtgatag gagatatccg caccgtgccg gttgaactcg tgcaccagct
     8041 cggccatcac ctcgtattcc atgcgcccgg gccggcagaa acgcatggct cgcacgtgcg
     8101 caccggcggc gatctgtccg gagcggcgca gcagcgacac ttcggcgcgg ctcttgaaca
     8161 gccgcatgtc atgcaacaca tgatcgagcg caacgaactc ctgcggagag tgcctgccgg
     8221 tgcgtgcctg ggcgcgcagc gtgttcaccc aactcaccac atgatggtcg aactcctggt
     8281 tcagtcccat cgtgtagaac acgcgtgatc ggttttcgag caggccgggc agaatctcgt
     8341 cgatatcgca gatcgggaag gcgtcgtcgg cacggtattc ctcgaccgcg ccggccggac
     8401 cggcgcgctt gccgtcccag atctcgcgat cagggtcacg ctcgcgaatg aacagaatgt
     8461 attgcgcggc cggacgcccc gggatcagca ccgcgaccgc atcgggttcg ctgaagcctg
     8521 tcagatagta gaaatcgctg tcctgacggt agttgtagtc cacgtcgttg ttgcgctggc
     8581 gcaccggtgc cgccggcagg attgcaatgc cctcctcgcc gatcaggcgc atcagctggg
     8641 tgcgccggcg ttcgaattca tccagcgcgg agcgtgagcg gggcatgaag acctttaatg
     8701 caagacgtga tcgggtggag tgtggcgctc gcgcagagga ctgagttcgt cgaacaccag
     8761 ctgcactccg acgcggacga attccaccag ctcggcgagc gcggattcat tcgcctcctg
     8821 agtatcaccg gcatccacgt cggcgcgggt aatttccgtc agatcacgca cgatttcacc
     8881 cagctctccg ggcaggcgct gcggatccgc ggcgctggta cccagtccgt acaggaaccc
     8941 ggtgcaccac aaggtcagcg cctgcgtgcg ctcctcgatc ggccgatcgt cggccggaat
     9001 cagaatgtcg aattccatct caccgctcga cagggcggcg acggtcgcct ggtacaacgt
     9061 ctgcaacacc ggatcggcgg cagtggacag actgtcttcg ggcaggatct ctgccagcca
     9121 gtcctccatt ctgtaaggca ccagtgcaca cagtgccccc gccaattgac cgtgcgcctc
     9181 ggcggggtcc gccagagcgt gtgaatgcgc cagtgagcgt tgtatttcgg cgtagtcgtg
     9241 ctgaatggac acgacaaaat ggtatggcag ccgctgtatg ggccgcaacc atgattatga
     9301 cccgtttttc attgacctcg gcatccgcgg ggaactatag ttttcacatg gcagaagcaa
     9361 aaatacccag cgccatcgac ttggagctga agcgcctcga acgtcgagtc gatgaactgc
     9421 tgacaacggt ccaacagctc aaggaagaga atcgtgcact gcgtcagcgt caggatacgc
     9481 tcgcggccga gaagacttct ttgctcaacc gcaacgaaca ggtgcgcact cgcgttgagg
     9541 cgatgatcgg tcgactcaag gcgatggaac acagcgcatg agcgagcgcg aggcggttcg
     9601 cccgctcggc gtcaggatcc tcgaaaaaga atacatcgtt gcctgcggtc ccgatgagcg
     9661 cagcgaattg atcgatagcg ccgagttcct caacaaacgc atgaaagaga tccgcgacag
     9721 cggcaagatc atcggattgg acagaatcgc cgtgatggcg gcgctcaatc tggcgcacga
     9781 agtcatcaag atgcgggcgc gggataccgg cctcgaacag gaggccggac atcgcatcaa
     9841 gctgctgcgc gagcgggtcg agtctgcgct cgagaaagga caacagctcg agttgtgagc
     9901 ggatccgcat ccccggcata cgggggacac ctgctcaagc tttggtgtac agtggcctca
     9961 agcgtcgcct gcggtgttcg acaagtgggc tgggttacct ctcgacccta attgaaacac
    10021 ccaagggacc gattgccttg ctgacagcac tgtgcaggtc tgccacgcag cagaaagcct
    10081 tacctgtcgc aggtggtccc acttgttgct ccggtcgaga gcaacggtcc gcaccggcat
    10141 ctgcgggtgg cgccccttaa ctcccccgac aacgacatcg cggcgggcga gcgcttggaa
    10201 ctgcgtcgca cgctgcggcg ccggcgcgcc gatctgagcg cgcaacagcg cgcaacggcc
    10261 gctcgcgcca tcacctcaca cgttgccgcc acccgctggc tgcagggcgc gcgtccgata
    10321 gcgctgtacc tatcggtcgg ttccgaagtg gacaccggac cgctatcgga gctggcgcag
    10381 cggcggcgct gcccggtcta tctgccgcgt atcacggact atcgcaccca ccgcatgcag
    10441 ttcgtactgc tgtccgcggc tcagaacgag cgcaatcgac acggcatccc tgaaccgggc
    10501 ggtcgccgca gcatctgccc tggagcactg tcggtggtgt tcctgccggt actcggattc
    10561 gacgcgcgcg gtacacgcct gggctctggc gcaggctact acgatcgagt gttctcgttc
    10621 cggcgacatc ggctctcatg gcaccgtccg ttgttggtcg gggtctccta tcgatgccag
    10681 gagctgccct gcattgaacc gcagcgccat gacgtgccac tggatgcact ggtgactgag
    10741 gacggtgtaa ggtatttccg tcagggggga tgaccaccat gaatcactgg ctgctcaaga
    10801 ccgaacccac caccttcggt gtcgatcatc tggaagctgc ggcgcgtaag accaccggct
    10861 gggatggcgt gcgcaactat caggcgcgca actacatcag ggaccagatg aagaaagggg
    10921 acgaaggctt tctttatcat tccagctgcg cgatacccgg agtcgtcgcc atcgtgaccg
    10981 tcgtcaaagc gccgtacccc gatgaaaccg cgttcaaacg cggcgatccg cacttcgatc
    11041 cggacagtga ccccgccgct ccgcgctggt atagcatgga tatcaagctc acgcggcgat
    11101 tgaagcgcgt catcacgctc gaggaactga aggcgcatgc gaagaaacaa ctctcaggaa
    11161 tgatcctgtt gcggccaggc aatcgcctgt cggtctcgcc ggttgaagac gcgcactggc
    11221 gatttgttct atcgcttgag tgaacgaact cgttcgaagt ggtgtgtgag tgacatcaca
    11281 gacgcgcaca gctcctactc tcttgcttgt aattttgcaa cgcgtgtata tactcggacc
    11341 ccggactaac ctgacatgga g
//
LOCUS       DATMRM010000476         5496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_607299, whole genome shotgun sequence.
ACCESSION   DATMRM010000476 DATMRM010000000
VERSION     DATMRM010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607299"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VNZ06_14540"
     CDS             complement(<1..467)
                     /locus_tag="VNZ06_14540"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWW22014.1"
                     /translation="MSVATRTGVQRTALPLLGLSALGIVFGDIGTSPLYTLKTVLSLG
                     GNPPTQDAVLGVLSLIIWTLTLITTVKYVIVAMSIDHDGEGGILALMSLLSHKRHKRP
                     LIVAVGLLGAALIYGDGAITPAISVLSALEGLNDPLPAFRPYVLPAAVTVLVGL"
     gene            complement(536..1492)
                     /gene="ligD"
                     /locus_tag="VNZ06_14545"
     CDS             complement(536..1492)
                     /gene="ligD"
                     /locus_tag="VNZ06_14545"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HWW22015.1"
                     /translation="MASEILGVKLSHPDKALWPDGGNGKPVTKLEYARYLESVADWMM
                     PHLASRPCSLVRAPDGIARQLFFQRHAMKGASEHFGFVTVSGEREPYLQIDRAEGLIA
                     AAQIAALELHPWNCLPKSPEIPGRLVFDLDPAPDVNFVRVIEAALELRERLHRLGLTS
                     LCKTTGGKGLHVVCPLARERPALDWTAARQFAESLCSQMARDTPDKYLTSMVKKERTG
                     RIFLDYFRNERTATAVAPLSARARPVATVSMPLSWRQVRAGLDPARFTLHSAPALMAR
                     DKPWAGYTQAAGSLRAALAHLTRGVPRVHRARPARSPSRDAR"
     gene            complement(1621..2682)
                     /locus_tag="VNZ06_14550"
     CDS             complement(1621..2682)
                     /locus_tag="VNZ06_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22016.1"
                     /translation="MPRVRRRGHGLGNELLPWARAFLAAQVLDARLLAPAFGMNRRGY
                     WRDFYTAPDDWIYQRALEHLLPVVEFTESDWLAHGGGDVVSSFRSFATERKLFDRDFF
                     VLVTDGLWGGFRHVEAAREFMRSTLYTSRYAAHNLIQLHQRLDARKIQVAMHVRLGDF
                     VPQVAVDQYRKVANASLPIEWFCNVATALRQAFADDWQLLLITDGRAEQLAPLTSAFR
                     CITTADMTHNDCSDVLALAAADLLVCSASTYSSLAAFLSESPYLWFAPSLYAHPEGCF
                     STHGFAAETQDPNGPTRQAVDEYVRRAGIWPARGAVIALEGRIPASALEAAAQRRALR
                     RASSDLVRSGVADRQQAGD"
     gene            3070..4446
                     /locus_tag="VNZ06_14555"
     CDS             3070..4446
                     /locus_tag="VNZ06_14555"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HWW22017.1"
                     /translation="MSVLKTGQIAALIFAFLLSRAVAAQTAGLDTPADPRISMALKEI
                     SAEHVHATIAKLVSFGTRSTLSAQDPASIAAGRGIGAAREWLKSEFERYSKECGGCLE
                     VKVDAFIQPAVERVKEPTPLTNIYAVLKGTDPSPHIVLVTGHYDSRNSDNFNVSRDAP
                     GANDDASGTAVSLECARVLSKLKFRQTIIFLTVPGEEQGLLGSKHLAQIARQEGWPLD
                     AVLNNDIVGGNKGPDQDAQVVRVFSEGVPRQIDEQHLRAIEALGGENDSTSRELARYV
                     AATADTYRTSVKPLMIFRLDRFLRGGDHASFNEQGFSAVRFTEYREDFTRQHQDIRVE
                     NGINYGDTLDHVDFDYVANVARVNAATLASIASAPVAPTNVKIELTNLDNDSALSWDA
                     SPGASDYEVLWRATSSPQWEHAQKLGALTRTTLKISKDNVIFAVRALDKAGHASLPVV
                     PVPPPRGR"
     gene            4459..5124
                     /locus_tag="VNZ06_14560"
     CDS             4459..5124
                     /locus_tag="VNZ06_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22018.1"
                     /translation="MRRFARHASMPLGALAFWVCMLVAARRFPTPYDWRYITTSQLIY
                     PERNPAGHLWASAALMTCAGAGLLWVWTVRSTVPFRRLWVLAAGYACMTLSSLLPERW
                     HSIRNGHEILAIAGFVGVCCGLVLVSLNLRFPRRYPTRAPQVVLALIAFSPIAMAAIT
                     QVYLTWWRPEVPWVGPAWRTLGLPFYLSFAFWEWVTCALLSAYMTGIGLAVGEQACKT
                     SSS"
BASE COUNT         1045 a         1740 c         1723 g          988 t
ORIGIN      
        1 agccccacca ggacggtaac ggctgcgggc agcacgtatg ggcgaaacgc cggcagcgga
       61 tcgttcaagc cttcgagcgc ggacagcacc gagatcgccg gtgtgatcgc gccatcgcca
      121 tagatcagcg ctgcgccgag cagaccgacc gcgacgatca gcggccgctt gtgtcgcttg
      181 tgtgacagca gcgacatcag ggcgaggatg ccgccttcgc catcgtgatc gatgctcatc
      241 gcgacgatca catatttcac agtcgtgatc agcgtcagtg tccagatgat cagcgacagc
      301 acgccgagga cggcatcctg ggtcggagga ttgccgccca gactgagcac cgtcttgagg
      361 gtatacagcg ggctggtgcc gatgtcgccg aagacgattc cgagcgcgga cagacccagc
      421 agcggcagag cggtgcgctg gaccccggtg cgggtggcca cggacatgct tcatttcctt
      481 tgcggcgcgg ccgcacgccg gtcgcgctgc cggcaccgga gcggatacta cgtgctcagc
      541 gcgcgtcgcg cgatgggctg cgagcgggac gtgccctgtg tacccgcggc acgcccctcg
      601 tcagatgcgc cagtgccgct ctgagcgatc ccgccgcctg tgtgtaccca gcccacggtt
      661 tgtcgcgggc catgagtgcg ggtgcactgt gcagcgtgaa gcgcgccgga tccagacccg
      721 ctcgaacctg ccgccatgac agcggcatcg acaccgttgc taccgggcgt gcgcgcgctg
      781 acagtggcgc gaccgccgtg gccgtgcgct cattacgaaa gtagtcgaga aagatgcgcc
      841 cggtgcgctc cttcttgacc atgctcgtca ggtatttgtc cggggtgtcc cgcgccatct
      901 gcgagcacag gctttcagca aactgtcggg cagcggtcca atccagtgcg ggccgctctc
      961 gcgccagcgg acacaccacg tgcagtccct tgccacccgt ggttttgcaa aggctcgtca
     1021 ggccaaggcg gtgcagccgt tcgcgcagct ctagcgcggc ttcgatgact cgcacaaagt
     1081 tcacatccgg tgcgggatcc agatcgaaca ccagacgccc gggaatctcc ggcgacttgg
     1141 gcaggcagtt ccaaggatgc agctcgagcg ccgcaatctg tgccgcggcg atcaggcctt
     1201 cggcccgatc gatctgcaga tacggctcgc gctcgccgct gactgtcacg aatccgaaat
     1261 gttcggacgc ccccttcatg gcgtgacgct ggaagaacag ctgccgggcg atgccatcgg
     1321 gtgcccgcac cagggaacac ggacgcgatg ccaggtgcgg catcatccag tcagccaccg
     1381 attccagata gcgcgcgtac tcgagcttcg tcaccggttt gccgttgcca ccgtccggcc
     1441 acagcgcttt gtcggggtgg gacagcttca cgcccagaat ttcgctcgcc acgctttgat
     1501 ccttgcgctg actgcgcgta tgaattgtgt gtcggcggag ctcaacgccg ctgtcggaca
     1561 gttctgaatc cagggttgaa ggcgcttaga gggtctgccg ccgcagggtg ccaccagtcc
     1621 ttagtcccca gcctgctgtc ggtccgcgac gccggatcgg accagatccg agctggcccg
     1681 ccgcagcgcc cggcgttgtg cggccgcctc cagtgcgcta gcgggaatgc gtccctccag
     1741 agcgataacc gccccgcgcg ctggccaaat gcccgcgcgt cggacgtatt cgtcgaccgc
     1801 ctggcgcgtg ggcccgttcg gatcctgtgt ttcggctgca aaaccgtggg tggagaagca
     1861 gccctccggg tgcgcgtaca gactcggcgc aaaccaaaga tacggcgatt cggacagaaa
     1921 ggccgccagc gagctgtagg ttgaggccga acagaccagc agatccgctg ccgccagtgc
     1981 cagcacatcc gagcagtcgt tgtgtgtcat gtcagccgtg gtgatgcacc gaaaggcgct
     2041 ggtgagtggt gcgagctgct cggcgcgccc atcggtgatc agcagcaact gccagtcatc
     2101 ggcgaaggcc tgacgcagag cggtcgcgac attgcagaac cactcaatgg gtagcgatgc
     2161 gttcgcgact tttcgatact gatcgaccgc aacctgtggc acgaaatcac cgagtcgcac
     2221 gtgcatcgca acctgaatct tgcgcgcgtc caaacgctgg tgcagctgga tcagattgtg
     2281 ggccgcgtag cggctcgtat acagcgtcga ccgcatgaat tcgcgcgcgg cctccacatg
     2341 ccgaaaccct ccccacaggc cgtcggtgac gagcacgaag aagtcgcggt cgaagagctt
     2401 gcgctcagtc gcgaagctgc gaaaggagct cacgacatcg ccgccgccgt gcgccagcca
     2461 atcgctctcc gtgaattcaa ccacgggcag caggtgctcg agcgcgcgct gatagatcca
     2521 gtcatccgga gcggtgtaga aatcgcgcca gtaaccgcgc cggttcatcc cgaaggccgg
     2581 agccagcagt cgggcatcga gtacctgcgc ggccagaaaa gcgcgggccc acggcaacag
     2641 ttcgttgccg aggccgtgtc cacgccgacg cacgcgcggc atgcagaacc gcagcactac
     2701 cgggtgcccg ctgaacgtcg acggttggac atgggtgaga gatattctag ccgcaggtgt
     2761 ccgccgcaac atttcgtgtc agcgctgtgt tctgaatcgg ggtttgaccg gtttcctatt
     2821 cgagttaatt ggtaataatg cattgtaatc atttaaaaca tatatataaa tcatcgattt
     2881 tcatgccaag agaacgcgcc gtcatgactt tcttcaagac ctgccgaagg gcggcttgct
     2941 cgcgccgcgt cgcctgagcg gcgcagtatg ggcacagacg tgcgagcttg aaatgccggt
     3001 cgatccaatc agggcttgaa cgccagattg atagactgcc ttcacttcac accaggggaa
     3061 cgcagcgtga tgagtgtgct caagaccggt caaatagcgg cgctgatctt cgcattcctt
     3121 ctgagccgtg cagtcgcagc gcagaccgca ggcctcgata ctcctgccga tccaagaatc
     3181 agcatggccc tgaaggaaat ctcggccgag catgtccacg ccacgattgc caaactggtc
     3241 agtttcggaa cccgctcaac gctgtccgcg caggatccgg cgtccattgc tgccggacgc
     3301 ggtattggcg cggcgcgtga atggctcaaa tccgagttcg aacgctactc gaaggagtgt
     3361 ggcggctgcc tcgaggtcaa agtggatgca ttcatccagc ccgcggtgga gcgcgtcaag
     3421 gagcccacgc ccctcaccaa catctacgcg gtgctgaagg gcactgaccc gtcgccacac
     3481 atcgtgctcg tgaccgggca ctacgattcg cgcaacagcg acaacttcaa cgtctccagg
     3541 gatgcgccgg gcgcaaacga tgacgccagt ggaacggcgg tcagcctgga atgcgcgcgc
     3601 gtgctgagca agctgaaatt caggcagacg atcatcttcc tgacggtccc cggggaagag
     3661 caggggctgc tcggtagcaa gcatctggcg cagatagcca ggcaggaagg ctggccgctc
     3721 gacgccgtac tcaacaacga tatcgtcggc ggcaacaagg gaccggatca ggatgcgcag
     3781 gtggtacgcg tgttctcgga aggcgtgccg cgtcagatcg atgaacagca tctgcgtgcg
     3841 atcgaagcgc ttggcggcga aaatgactcc acctcccgtg agttggcgcg ttacgtcgct
     3901 gcgaccgccg acacctatcg caccagcgtg aagccactga tgatcttccg gctcgatcgc
     3961 ttcctgcgcg gtggcgatca cgcatccttc aacgagcagg gcttcagcgc cgtgcgcttc
     4021 actgaatacc gcgaggactt tacccgccag catcaggata tccgtgtcga aaatggaatc
     4081 aactacggcg acacgctcga ccacgttgat tttgactatg tggccaacgt ggcacgcgtc
     4141 aatgcggcga cccttgcatc gattgcctcg gcgccggtgg cacccaccaa tgtcaaaatc
     4201 gagctgacca atctcgacaa cgatagcgcc ttgagctggg atgcctcacc gggcgcgagc
     4261 gactatgagg tcctgtggcg cgcgaccagc agcccgcaat gggagcacgc ccagaagcta
     4321 ggcgcgctca ccaggaccac gctcaagatc tccaaggaca atgtaatttt cgccgtgcgc
     4381 gcactcgaca aggcgggcca tgccagcctc ccggtggtgc ccgtcccacc gccaagaggg
     4441 cgctgatccg acgccgtgat gcgtcgcttt gcccgacacg caagtatgcc actgggtgcg
     4501 ctcgccttct gggtgtgcat gctggtggcg gcgcggcgct ttccgacgcc ctacgactgg
     4561 cgttacatca cgacctcaca gctgatctat ccggagcgta atccagcggg gcatctgtgg
     4621 gcctcggcgg cactgatgac ctgcgccggg gccggcctgc tgtgggtgtg gaccgtgcgc
     4681 tcaacggtgc ctttccgcag gctctgggta ctcgccgccg gctacgcctg catgactttg
     4741 agctcgctgc tgcccgaacg ctggcattcg attcgcaacg gtcacgagat cctggcgata
     4801 gccggcttcg tcggcgtgtg ttgcggtttg gtgctggtct cactcaacct caggtttccg
     4861 cgacgctatc ccacgcgagc ccctcaggtc gtgctcgcgc tgatcgcctt ttccccgatc
     4921 gcaatggccg ccatcacaca ggtctacctc acgtggtggc ggccggaggt cccgtgggtg
     4981 ggacccgcct ggcgaaccct ggggctgccg ttctacttga gttttgcctt ttgggaatgg
     5041 gtaacatgcg cactactgtc cgcctatatg accggcatcg gcttggcagt aggggaacaa
     5101 gcgtgcaaga cgtccagctc ctgatcgcag gccgtgacag gcatgccgcg ggcggcagga
     5161 gcttcacacg tcgcaatcca atcgacggct cggtagccac ccgcgcggca gcggcgaggt
     5221 cgcgatcgag gccttcacgc agctgcgctg gacaacgatc cagaccacac acgcggactg
     5281 acaggaaagt ttgaaagtcg cttgcctcaa ggccaatata ggcgtgttcc gaccacaaca
     5341 acgaccaaca acagaacgag aataggggga acacgatgtt caatcagatc ctgtcgccgg
     5401 tgggcggcaa cctggcgctt tcgtttgtgt gcgcggcgct gccgatcgcg acggttctga
     5461 ttgcactggg tgtactgcga cggccggctt ggcagg
//
LOCUS       DATMRM010000477         2781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3050987, whole genome shotgun sequence.
ACCESSION   DATMRM010000477 DATMRM010000000
VERSION     DATMRM010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3050987"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..432
                     /locus_tag="VNZ06_14565"
     CDS             238..432
                     /locus_tag="VNZ06_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22019.1"
                     /translation="MDEKTRSSLARIAAEWQKGAASRVEEDADEVESRRAVNESVAMS
                     PIALRHRRYAQLIQFRRRAE"
     gene            569..1816
                     /locus_tag="VNZ06_14570"
     CDS             569..1816
                     /locus_tag="VNZ06_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22020.1"
                     /translation="MQITLGPEIIALDGPWKFRIGDNLRWAAPDFDDAVWESLDLTPQ
                     FGAHDADVGLMGYVPGWEARGHRGYSGYAWYRLRVSVTAPPGQPLALVGPTNVDSAYQ
                     IYLDGRLLGGIGDFTHSPPAAYGTNRPATFPLVSTAGPMLVAVRVWMGSWDLNDSDSG
                     GIHIAPALGTKAGAEALYQIQWMELIRGYFLEIVLAVGFVVLAAMVCSLMSLTDFKSA
                     HLWLSAALLCVALMRANQAVFFWGQFETVKVFELITNVLLVPLMLGAWTIAWASWLNL
                     KSRRWIVPTASVLTMLHIALQCLRASWFHGAFPKAIETALGSCVAGVRLLFVLLTLQI
                     LVSVVRQPDRERWFALCAIVLICVGLFAQELSQLGVPGIWFPFGTGVSRTQYAYAAFD
                     IVFFALILQRLMQTGNRPVIASR"
     gene            complement(1838..>2781)
                     /locus_tag="VNZ06_14575"
     CDS             complement(1838..>2781)
                     /locus_tag="VNZ06_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22021.1"
                     /translation="AIARVSTPTVSPSAAVARAPSSAPPARTNVEDPQIPALLAKLST
                     VPAPDDVSADAALGDLQGLEALVGRNDERVRQVRAHISRAYLQQSQALLNERRLSEAQ
                     RVLDLSQKFGLAPAAYITQENALRQVRKQVQADNQKVSATEETAASQTRILDQARADQ
                     IDQAEAQLAVLTKTLPAGDPFITTKAPQAIGDAYLSRAQKFFAQARFEEALQSARAAQ
                     LAAPGWVQFAAAAQRYQDARSLAVTLSSIRDPREFDKFKPVLDRLRAVDPAEYPSFES
                     GFVHILSDRINALDPASAAKLRSSVLRLFPEASIGQP"
BASE COUNT          449 a          852 c          916 g          564 t
ORIGIN      
        1 ccgggacgcc aagacgatca cggagatcgc caatcgcggg tccgtcagcg aggaccgtgc
       61 gcaattcgga catttgctaa gactgatcct ggatgcgcga accggcagcg caggagcggc
      121 ggcttccccg acacgctaag catcgcggtc ggaacgttgg catgaacccg agggcttccc
      181 ccggtcttat gtctcgcgaa cacgaaattg caaccgatat agggtagact accccccatg
      241 gacgaaaaga ccagaagttc actcgcccgc attgcggccg agtggcaaaa gggtgctgcg
      301 tcgcgggtgg aagaggacgc cgacgaggtt gaatcccgtc gggcggtcaa tgaatctgtg
      361 gctatgtcgc cgatcgcgct gcgccatcgg cgatatgccc agctcatcca gttccgccgc
      421 cgggcggaat agcccgtcga gcccagcttc gacccaggcc tgccggctgc cggcaggcct
      481 tttgcgttgg tgccgaagcg gctgtgtact gctcgtcctg tgcctatcgc ttttctgccc
      541 gcgcctgccg gcagccacgg cggcgcccgt gcagatcacg ctcggtccgg agatcatcgc
      601 gctcgacgga ccgtggaaat ttcgcatcgg tgacaatctg cggtgggccg cgccggattt
      661 cgatgacgcg gtatgggaga gcctggacct cacgccgcag ttcggcgcgc acgatgcgga
      721 tgtcggattg atggggtacg tgcccggctg ggaggcgcgc ggacaccgcg gttattccgg
      781 ttacgcgtgg tatcgcttgc gcgtgtcggt cacggcgcca cccggacagc cgctcgcgct
      841 ggtcgggccg acaaatgtgg acagtgccta tcagatctac ctggatggcc ggctgctggg
      901 aggcatcggc gactttacgc attcgcctcc tgcggcgtac ggcaccaatc gaccagccac
      961 tttcccactg gtgtcaaccg cgggtccgat gttggttgcc gtgcgtgtct ggatgggttc
     1021 gtgggatctg aatgactcgg acagcggcgg gattcatatc gctcccgcgc tgggtaccaa
     1081 ggccggggct gaagcgctct atcagattca gtggatggaa ctcatccgcg gctacttcct
     1141 cgagatcgtg ctggcagtgg gttttgtggt gctcgccgcg atggtttgca gtctgatgtc
     1201 actgactgac ttcaagtcgg cacatctgtg gctttcggcg gcgttgctgt gtgtcgcgct
     1261 gatgcgcgcc aaccaggctg tgttcttctg gggacagttt gagacggtga aggtattcga
     1321 actcatcacg aatgtgctgc tggtgccgct gatgctggga gcgtggacca tcgcctgggc
     1381 gtcgtggctg aatctaaagt cgcgccgctg gatcgtcccc actgcgtcgg tgctgacgat
     1441 gctgcacatc gcgctgcagt gtctgcgcgc gtcctggttc cacggcgcct ttccgaaagc
     1501 catagaaact gcgctcggat cgtgcgtggc aggggtgcgg ctgttgttcg tgctgctgac
     1561 gctgcagatt ctggtgagcg tcgtgcgcca gcccgatcgc gagagatggt tcgcgctctg
     1621 cgccattgtc ctgatctgcg ttgggctgtt cgcccaggag ctgtcgcaac tgggggtacc
     1681 cggcatctgg tttccgttcg gcactggcgt atcacgcacg caatacgcct acgccgcctt
     1741 cgacatcgtg tttttcgcgc tgatcctgca gcgattgatg cagactggca accgccctgt
     1801 catcgcatcg aggtgacgac gtggtcgaac gcgcagttta gggttgcccg atgctcgcct
     1861 cggggaacag ccgcaagacg ctgctgcgca gtttcgccgc ggaagccgga tccaacgcgt
     1921 tgatgcgatc ggagagaata tgcacgaaac cggattcgaa cgacggatac tccgccgggt
     1981 ccacggcgcg caggcgatcc agcaccggct tgaacttgtc gaactctctc ggatcgcgga
     2041 tggaactgag tgtcacggcg agcgagcgcg catcctgata gcgctgtgca gccgccgcaa
     2101 actgcaccca tccgggagca gccagttgcg ccgcacgcgc cgattgcaat gcctcttcaa
     2161 aacgcgcctg ggcaaagaat ttctgtgcgc gcgacaggta ggcatcgccg atcgcctgcg
     2221 gcgccttggt ggtgatgaaa ggatctcccg ccggcagcgt cttggtcagt accgcgagct
     2281 gggcttcggc ctgatcgatc tgatcggcac gcgcctggtc gagaatgcga gtctgggaag
     2341 ccgccgtctc ctcggttgca ctcactttct gattgtcagc ctgtacctgc ttgcggactt
     2401 gtcgcagcgc attctcctgc gtgatgtaag ccgccggagc caacccgaac ttctggctca
     2461 ggtccagcac gcgctgcgct tcgctcaggc gccgctcgtt cagcagcgcc tgcgactgtt
     2521 gcaggtaggc gcggctgatg tgcgcgcgca cctgacgcac gcgttcgtcg ttacggccca
     2581 cgagcgcctc gagaccctgc aggtcgccca atgcggcgtc ggcggacaca tcgtccgggg
     2641 cgggtaccgt ggacagcttc gccagcaaag caggtatctg cggatcttcg acgttggtgc
     2701 gcgccggtgg cgcgctgctc ggtgccctgg caaccgccgc gcttgggctg acggtcggtg
     2761 tactgactcg cgcgatcgcc g
//
LOCUS       DATMRM010000478         4882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3360179, whole genome shotgun sequence.
ACCESSION   DATMRM010000478 DATMRM010000000
VERSION     DATMRM010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3360179"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VNZ06_14580"
     CDS             <1..971
                     /locus_tag="VNZ06_14580"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HWW22022.1"
                     /translation="VKVAAMIDQKRPDGSLRHLITLDGLRRDEIDTLLQRSQQFARRT
                     GDRPPTSRSLKGVTVATLFTEPSTRTRVSFELAGRRLGAQVLNLELQLSSRVKGESML
                     DTIFTLQALHFDVFVIRDSEPGVTALVAANVAPKVSVLSAGEAHLTHPTQGLLDALTI
                     RQHKGPLEDLRIAIVGDIRHSRVARSAYQVFRILEARETRIVAPPALMPEAQEFAGCS
                     RFETLEEGLAGVDVVMMLRIQKERIAETSIPDGDEYFARYGLTPARLARAHPGAIVMH
                     PQPMNRGIEIASEVADGPQSVIRDQVSNGVAVRMAVLESVAARLNA"
     gene            968..1873
                     /locus_tag="VNZ06_14585"
     CDS             968..1873
                     /locus_tag="VNZ06_14585"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HWW22023.1"
                     /translation="MSELPAHRGTICLEQARVLQHTHLEADQHILRLHAPRCAARATP
                     GSFVHLRCSDELPMRRPLSIMRADKKAGSVDVLYKVIGPGTRLLAKAQVDEQLNVLGP
                     IGQGFVPHPQRPRVVAIGGGVGIPPMVFLVERLADDEELAWKPLVLMGSEIPFPFRTR
                     PSTILLPGMPDAVIACMPLLDEWGVPSRLASLAGYPGCYDGYVTDLARVWLRTLTAQQ
                     LQETELFACGPTPMLSATARLAREFLLPCQVALEEFMACAVGGCAGCTVAVTTAAGTA
                     MKRVCVDGPVFEADSVFPATPASRS"
     gene            complement(1860..2963)
                     /locus_tag="VNZ06_14590"
     CDS             complement(1860..2963)
                     /locus_tag="VNZ06_14590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HWW22024.1"
                     /translation="MAVDPLEITAAEAQPTVNTKPLFKLMVERKASDLFFTSNAPIKI
                     KIEGQIYPINKQVLPPAAVRSICFGLMTTRQIEHFQKELEIDFAISEPGLGRFRVNIF
                     FQRGYPAMVLRYITADMPRLDVLGLPEVLTELAMIKRGLILLVGATGSGKSTTLAAMI
                     NHRNENASEHIVTIEDPIEFLHTNKRSIINQREVGLDTKSYDRALRGVMRAAPDVILI
                     GEIRDRESMQAAINLSGTGHLVLATLHANNCAETLDRIINMFPLDQHKQVELDLSQYL
                     RAILAQRLVIGVDGKRVAAVEVMLNTPHISELVKRGDVTGIKESIMQSGEKEVCSFDS
                     ALHRLYKEGRIGLDEALANADSRTNLEAKINFG"
     gene            complement(2971..4125)
                     /locus_tag="VNZ06_14595"
     CDS             complement(2971..4125)
                     /locus_tag="VNZ06_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HWW22025.1"
                     /translation="MDRDQAIKLMQELLKRVVERAASDLFITAGFPPAIKIDGEIRPQ
                     MERALTSDQSAILVRAIMNDRQTKEFDASKECNFAIAPPGIGRFRVSAFVQQGHVGCV
                     IRMINAKIPSFEELDLPPVLKEVVLSKRGLVIVVGGTGSGKSTTLASMVGYRNDKTRG
                     HIVTIEDPVEYVHQHKGCVITHREVGVDTESWHAALKNTLRQAPDVILIGEIRDRETM
                     EYGIQFAETGHLVLATLHANSSNQALDRIINFFPEERREQLLMDMSLNIRGLISQRLI
                     PRESGSGRIAAMEIMLNSPLIQDLIFRGEVAKIKEVMSRSTRIGMKTFDQALFELYEQ
                     GHISYEDALRNADSKNELRLRVKLESKRESKPVESDGDLTIAEEVQGKNF"
     gene            complement(4152..>4882)
                     /locus_tag="VNZ06_14600"
     CDS             complement(4152..>4882)
                     /locus_tag="VNZ06_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HWW22026.1"
                     /translation="ILSLDELQSPKIFRDLCMLPRGLVLVTGPTGSGKSTTLAGMIDY
                     CNDNRPDHIITIEDPIEFVHESKRCLLNQREVKRDTLGFSEALRSALREDPDIILVGE
                     LRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRVVDVFPGEEKDMVRSMLSESLRA
                     VISQTLLKRTGGGRVAAHEIMIGTPAIRNLIREGKIAQMYSSIQTGQSAGMQTLDQCL
                     TELVSKGIVSKDEARYKASNKDAL"
BASE COUNT          851 a         1534 c         1508 g          989 t
ORIGIN      
        1 gggtgaaggt cgccgcgatg attgatcaga aacgtccgga cggttcgctg cgccacctga
       61 ttacgttgga cggcttgcga cgtgatgaaa tcgacacgtt gctgcagcgc tcgcagcagt
      121 ttgcccgccg taccggcgat cggccgccca cctcccgttc gctcaaggga gtgacggtcg
      181 cgaccttgtt caccgaaccc tcgacccgta cgcgcgtctc gttcgagctg gcgggacggc
      241 gactgggcgc gcaggtgctc aatctcgaac tgcagctatc ctcgcgagtg aagggcgaga
      301 gcatgctcga tacgattttc acgctgcagg cgctgcattt cgatgtgttt gtgatccgtg
      361 attcggaacc cggcgtcacg gcgctggtgg ccgccaacgt ggcgccaaaa gtcagcgtgc
      421 tgtcggcggg cgaggcgcac ctgacgcatc ccacccaggg gttgctggat gcgctcacga
      481 tccgtcagca caaggggccc ctggaggacc tgcggatcgc gatcgtcggc gacatccgcc
      541 attcgcgcgt cgcccgatcc gcctaccagg tgttcaggat cctcgaagcg cgcgagaccc
      601 gcatcgtggc cccgccggcg ctgatgcccg aggcacagga attcgctggc tgctcgcgtt
      661 ttgagacgct ggaggaagga ctcgcgggcg tcgatgtcgt gatgatgctt cgaattcaga
      721 aggagcgtat cgccgagacc tcgattcccg acggtgatga gtatttcgcg cgctacggcc
      781 tgacgcctgc gcgactggcg cgcgcccatc ccggggcgat cgtcatgcat ccgcagccga
      841 tgaatcgcgg catcgagatc gcctccgagg ttgcggacgg tccacagtct gtgatccgcg
      901 atcaggtcag caatggcgtg gccgtgcgga tggcggtgct cgaaagcgtc gccgcaaggc
      961 tgaacgcatg agcgagctgc ctgcccaccg cggcacaatc tgcctggagc aggcccgcgt
     1021 gctgcagcac acgcacctgg aggccgatca gcatatcctg cgattgcacg cgccccggtg
     1081 cgctgcgcgg gcgacgcccg gcagcttcgt gcacctgcgc tgctcggacg aactgccgat
     1141 gcgacgaccg ctgtcgatca tgcgcgccga caagaaggcc ggatcggtgg atgtgctcta
     1201 caaggtgatc gggccgggaa cgcgcctgct ggccaaagcg caggttgatg agcagctgaa
     1261 tgttcttggc ccaatcggcc aaggcttcgt accgcatccg caacgcccgc gcgtcgtcgc
     1321 gatcggtggt ggtgtgggca ttccgccaat ggtctttctg gtcgaacggc tcgccgatga
     1381 cgaggagctc gcctggaaac cgctggtgct gatgggctcg gagattccgt tcccgttccg
     1441 cacccgcccc tcgacgatcc tgttgccggg tatgccggac gccgtgattg cctgcatgcc
     1501 gctgctcgac gaatggggtg taccctcgcg cctcgcctcc cttgccggct atccgggctg
     1561 ctacgacggc tatgtgaccg acctggcgcg cgtctggctg cgtaccctga cggcgcagca
     1621 gctgcaggaa accgaactct tcgcctgcgg accgacgccg atgctgtcgg ccaccgctcg
     1681 gctcgcgcgt gagttcctgc taccctgcca ggtggcgctc gaagaattca tggcttgcgc
     1741 cgtcggcggc tgtgccggat gcaccgtcgc ggtaacgacc gcggccggga ccgcgatgaa
     1801 gcgggtttgc gtggatggac cggtattcga agccgactcg gtctttccgg cgacgccggc
     1861 tagccgaagt tgatcttcgc ttccagattg gtcctggagt ccgcgttcgc cagggcctcg
     1921 tcgagaccga tccgcccttc cttgtacaac cgatgtagcg cgctatcgaa gctgcagact
     1981 tccttttcgc cgctctgcat gatcgactcc ttgatcccgg tgacgtcgcc acgcttgacc
     2041 agttcggaaa tgtgcggcgt attgagcatg acctcgaccg cggccacgcg cttgccgtcc
     2101 acgccgatca ccagccgctg cgccagaatc gcccgcaggt attgcgacag gtccagctcc
     2161 acctgcttgt gctggtcgag cgggaacatg ttgatgattc gatcgagggt ttcggcgcag
     2221 ttattggcgt gcagggtcgc cagcaccagg tgtccggtac ccgacaggtt gatagcggcc
     2281 tgcatgctct cgcggtcacg aatttcaccg atcagaatca catcgggtgc cgcgcgcatt
     2341 acgccgcgca acgcacgatc gtagctcttg gtatccagac cgacctcgcg ctgattgatg
     2401 atcgagcgct tgttggtgtg caggaactcg atcggatcct cgatcgtcac gatgtgctcg
     2461 gacgcattct cattgcgatg attgatcatc gcggccagcg tggtggactt acccgagccg
     2521 gtcgcgccga ccagcaggat cagcccacgc ttgatcattg ccagctctgt cagcacctcc
     2581 ggcaatccca acacgtccag ccgcggcatg tcagcggtga tgtagcgcag caccatcgcc
     2641 gggtagccgc gctggaagaa gatgttgacg cggaagcggc ccagccccgg ctcggaaatg
     2701 gcgaagtcga tctccagctc tttctggaaa tgctcgatct gtcgcgtggt catcagccca
     2761 aagcagatcg agcgtaccgc cgccggcggc aatacctgct tgttgatcgg gtagatctgg
     2821 ccttcgatct ttatcttgat gggcgcgttg gaggtaaaaa acaggtccga cgccttgcgc
     2881 tccaccatca gcttgaacag cggcttggtg ttgacggttg gctgcgcttc cgcagcggtt
     2941 atctcgagcg gatctacggc catggggttg ctagaaattc tttccctgta cttcctcggc
     3001 gatcgtcaga tctccgtcgc tctcgaccgg cttgctctcg cgcttgctct ccagcttcac
     3061 ccgcagacgc agctcattct tcgagtcggc gttacgcagg gcatcttcat aagaaatgtg
     3121 gccctgctca tacagttcga acagtgcctg atcgaaggtc ttcatgccga tgcgggtcga
     3181 acgcgacatg acctctttga tcttcgctac ttcaccacgg aagatcaggt cctgaatcag
     3241 cggtgagttc agcatgatct ccatcgcggc gatgcggcct gatccggact cgcgcggaat
     3301 caggcgctgc gaaatcagcc cgcgaatgtt cagcgacatg tccatcagga gctgctcgcg
     3361 gcgctcctcg ggaaagaagt tgataatgcg atcgagcgcc tggttggagc tgttcgcatg
     3421 caacgttgcc agcaccaggt ggccggtttc ggcaaactga atgccgtact ccatcgtttc
     3481 gcgatcccgg atttcgccga tcaggatcac gtccggggcc tgtcgcaggg tgttcttgag
     3541 cgccgcgtgc caggactcgg tgtccacgcc gacctcacgg tgcgtgatca cgcagccctt
     3601 gtgctgatgc acgtactcga ccggatcttc gatcgtgacg atgtggccgc gggtcttgtc
     3661 gttgcgatag ccgaccatcg aggccagcgt cgtggatttt cccgagccgg tgccaccgac
     3721 cacgatgacc aggccgcgct tggacagcac tacctctttg agcaccggcg gcagatccag
     3781 ctcctcgaac gaagggatct tggcattgat catacggatc acgcagccga catgaccctg
     3841 ttgcacgaat gcactgacgc ggaaacgccc gatgccgggc ggtgcaatcg cgaaattgca
     3901 ttccttgctg gcgtcgaact ccttggtctg acgatcattc atgatcgcgc gcaccaggat
     3961 ggcggactga tcggatgtca gcgcccgctc catctgcggt cgaatctcac cgtcgatctt
     4021 gatcgccggg ggaaacccgg cggtgatgaa caagtcggat gcggcgcgct caacgactcg
     4081 cttgagcaac tcctgcatca gtttgatggc ttggtcgcga tccatggcct gcctcgaatc
     4141 gacccgtgtc gctacagggc atccttatta gatgctttat aacgcgcttc gtccttgctc
     4201 acgatgccct tcgataccag ctccgtcagg cattgatcca gcgtctgcat gccagcggac
     4261 tggccggtct ggatcgagga gtacatctgc gcaatcttgc cctcgcggat caaattgcgg
     4321 atggccggtg ttccaatcat gatctcgtgt gccgcgacgc gcccgccacc ggtgcgcttc
     4381 aacagcgtct gggagatcac cgcgcgcagc gattcggaca acatcgagcg caccatatcc
     4441 ttctcctcgc cggggaacac gtccaccacg cggtcgatgg tcttggcggc ggaactggtg
     4501 tgcagcgtcc cgaacaccag gtgtccggtc tcagcggcgg tgagcgccag gcgaatggtc
     4561 tccaggtcgc gcaactcgcc gaccaggatg atatccggat cttcacgcag tgccgaacgc
     4621 agtgcctcgc tgaagccgag cgtgtcacgc ttgacctcgc gctggttcaa cagacagcgc
     4681 ttggactcat gtacgaactc gatcggatcc tcgatcgtaa tgatgtgatc cggacgattg
     4741 tcgttgcagt agtcgatcat cccggcgagc gtcgtggact tgccggagcc ggtcgggccg
     4801 gttaccagca ccagcccacg cggcagcatg cacaggtcgc ggaaaatctt gggcgactgc
     4861 agctcgtcga ggctgagaat cg
//
LOCUS       DATMRM010000479         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3991350, whole genome shotgun sequence.
ACCESSION   DATMRM010000479 DATMRM010000000
VERSION     DATMRM010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3991350"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VNZ06_14605"
     CDS             <1..494
                     /locus_tag="VNZ06_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22027.1"
                     /translation="LCGRRQLLALLLVAGLALATAYLGGMLFEMFAGRRLRTYSVNEA
                     ESNSIFYVFGGLLCSLPSLHRLLTVEPPEEPPATGRTLANLAVMHVGVIAFTAISFFI
                     FPFGAGRVGYLIMLLLIPILPTMRRRDSVVGTMIFGLLLLYLVYLTVKTYLEGTYDIF
                     FGG"
     gene            510..1610
                     /locus_tag="VNZ06_14610"
     CDS             510..1610
                     /locus_tag="VNZ06_14610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWW22028.1"
                     /translation="MRVALVISSLGPGGAERVMSLLADALAARGHQVSLITLAHAWDD
                     FYSLDPRVQRRGLGLVGDSTRILGAVRANIRRIRALRRAIRTAAPEAVLAFVTRMNVL
                     ALIACLGLHVRVVISERVDPTRHTEGLLWQALCTLTYQHADAVVVQTEGIARWFRARL
                     WRRERVVVIPNPVTRAVETEQTGLPPPQPFILGAGRLVHQKGFDILIRAFSLIAADMP
                     KLRLVIAGEGSDAQQLRQLALTLGLECRVLFLGNVGNLSVLMRNAEVFVLSSRYEGFP
                     NVLLEALASGLPVVSTDCPSGPREILGDGEFGLLVPCENPQALADALRRVATDADLRR
                     RLSGIGSRATAAYEFGRVVAGWEAVLSAKALR"
     gene            1619..>2160
                     /locus_tag="VNZ06_14615"
     CDS             1619..>2160
                     /locus_tag="VNZ06_14615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HWW22029.1"
                     /translation="MIDLKTAINSVVGLLDLEIRRKVEKPGAPYPVEMSRDEKALFQH
                     VKRNALTMVSDERLFATVLACKHVLDCGVDGDFVECGVWRGGNALLAAGIFKLYGAAR
                     KVYLFDTFSGMTSPAQVDTAATTGESARVAHLSNQRDTHNEWCYASLEDVKATFRQAD
                     LLNSNVVFVKGDVLNTLSVA"
BASE COUNT          366 a          631 c          686 g          477 t
ORIGIN      
        1 ggctgtgcgg ccgcagacag ttgctggcac tgctgctggt ggctgggctg gcgctcgcca
       61 cggcgtatct gggtggcatg ctgttcgaga tgtttgccgg tcgtcgcctg cgcacataca
      121 gcgtaaacga agccgaatcg aactccattt tttacgtctt cggcggcctg ctctgcagtc
      181 tgccgtccct ccaccggttg ctgacggtag agcctcccga ggagccgccg gccacgggcc
      241 gcacgctggc aaaccttgcc gtcatgcacg tgggcgtcat cgcgttcacc gcgatcagct
      301 ttttcatctt ccccttcggc gccgggcgtg tcggttacct catcatgctg cttctcatcc
      361 ccatcctccc caccatgcgc cggcgtgaca gcgttgtggg cacgatgatc ttcggcctgc
      421 tgcttcttta tctcgtgtac ctcacggtca aaacctatct tgagggaacc tacgacattt
      481 tcttcggcgg atagggccgg gtcaccgcca tgcgagtcgc attggtgatt tccagtcttg
      541 ggccaggcgg cgccgagcgg gtcatgtcgc tcttggccga tgcactggcg gcgcgcggtc
      601 accaggtctc gctgattaca ttggcgcacg cctgggacga tttctactcc ctcgatcctc
      661 gtgtgcagcg gcgaggtctg gggctcgtcg gagactctac ccgcatatta ggcgccgtcc
      721 gcgccaacat tcgtcggatt cgagcgctgc ggcgggcgat ccggacagcc gctccggagg
      781 ccgtgctggc gtttgtgaca cgcatgaacg tgctagcgct tatcgcatgc cttggcctgc
      841 acgtgcgtgt agtgatttct gagcgcgtcg atccgactag acacaccgaa gggctccttt
      901 ggcaagccct gtgcacgctg acgtaccagc acgcagatgc tgtagtagtg cagacggaag
      961 gaatcgcgcg gtggttccgt gcgcgattat ggcgacgcga acgcgtcgtc gtaataccta
     1021 acccagtgac acgcgctgta gagactgagc aaacgggctt gccaccgccg cagccgttta
     1081 ttctcggcgc ggggcgcctg gtgcaccaaa agggcttcga catattgatt cgagccttca
     1141 gtctgatcgc ggccgatatg ccgaagctgc gcttggtgat cgcgggcgag ggttccgatg
     1201 cacagcaact gcggcagttg gctcttactc ttgggcttga atgccgggtg cttttcttgg
     1261 gtaatgtagg caacttgtcc gtgctgatga ggaatgcgga ggttttcgtt ctgtcgtcgc
     1321 ggtacgaagg gtttccgaac gtactgctcg aggcgctggc gagcggcctt cccgttgtct
     1381 cgacggattg cccgagcggc cctcgggaga ttctgggcga cggcgaattc gggctcctgg
     1441 tcccttgcga gaatccgcag gcgctcgccg atgccctgcg ccgcgtcgcg accgacgcgg
     1501 atttgcgccg tcggctgtcc gggatcgggt cacgagccac cgcagcgtac gaattcggcc
     1561 gcgtggtggc cgggtgggaa gccgtgctct cagcgaaggc actcaggtag aaatttccat
     1621 gatcgacctg aagacagcga tcaattcggt cgtgggactg ctggatctgg agatccgcag
     1681 gaaggttgag aagccgggcg ccccgtatcc ggtcgaaatg tcccgcgacg aaaaagcgct
     1741 gttccagcac gtgaagcgca atgcgttgac aatggtttct gatgaaaggt tatttgcgac
     1801 tgtccttgcc tgcaagcacg tcctggactg cggagtggac ggggacttcg tggaatgcgg
     1861 cgtatggcgc gggggcaacg ccctgctggc ggccggcatc ttcaaactgt atggtgcggc
     1921 tcgaaaagtc tatctattcg atacgttcag cggcatgacg tccccggctc aggtcgacac
     1981 cgcggcgacg accggtgagt cggcccgtgt tgcgcacttg agcaatcaga gagacacgca
     2041 caacgaatgg tgttatgcat ctctcgaaga tgtcaaagcc acgtttcgtc aggcggatct
     2101 gttgaattcc aacgtcgtct tcgtcaaggg agatgtcctg aatacgcttt cagttgcgca
//
LOCUS       DATMRM010000480         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2447423, whole genome shotgun sequence.
ACCESSION   DATMRM010000480 DATMRM010000000
VERSION     DATMRM010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2447423"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VNZ06_14620"
     CDS             <1..202
                     /locus_tag="VNZ06_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22030.1"
                     /translation="LRLLSTNRAYRQKDYLHNKRILLRKIMVEGMGHAWSGGDERHPF
                     NDAAGPDASRMIWDFVSMFHRD"
     gene            419..586
                     /locus_tag="VNZ06_14625"
     CDS             419..586
                     /locus_tag="VNZ06_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22031.1"
                     /translation="MDEPAVQSEAAVQDCARALPEDPLEELIEMRYQQYLAQRALEEL
                     FENGALQALEG"
     gene            complement(681..1961)
                     /locus_tag="VNZ06_14630"
     CDS             complement(681..1961)
                     /locus_tag="VNZ06_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWW22032.1"
                     /translation="MPAYADLLSAAARWDLVNFVASIARTAAWEKDGSFGGAGFAANP
                     AQRGEYITRWEVCGLCHTQTDRTGIYNVDGAFLAGGMRVGYYPHGYSVSRNLTSDPET
                     GLGHWTVPQIIRALRDGQAPDRALNPFAMLWAQFHNFTDEDATAIATFLKTASRPVHN
                     PIPPKLAYGLVETIAMKFTRPLPATAPKALTYGDGNFAVDSTRGSPARVQAVLVDVQW
                     IILILALVLFVRAGPRPGIVASIGGVLVFAVAGVLLLVLSQWPAIRNMPPDPLVKAVN
                     AGDVKPDTSDMPAERVRMVERGHYLYSVSCAICHGTDGSGGTPISWRPFGTLWTRNIT
                     AHPQAGVGAWSDMELERAIRSGVSRDGRQLHWQGMTWDQLSNLDEEDLRAIVAYVRLL
                     PPVDRAVPLPRPPTAGDCETYTYYLEKSDQPGCR"
BASE COUNT          392 a          635 c          652 g          360 t
ORIGIN      
        1 gttgcggctc ttgagcacga accgcgcgta tcgccaaaag gattatctgc acaacaagcg
       61 cattctgctg cgcaagatca tggtggaagg aatgggccac gcctggagcg gtggcgatga
      121 gcgtcatcct ttcaacgatg ctgcgggccc tgatgcaagt cggatgatct gggatttcgt
      181 gtcgatgttt catcgcgatt gattcgaccc gttcgaaggc ccgtgcaccg atccggtgcg
      241 tggttgcaca ccttcagggc agacggggaa tcggaccgcg tgttgtcatg cctagtctcg
      301 caatcacacg gacgacagca cagtcgcgga cttcgctcgg gtggcacggc atatgcactg
      361 ccaacccaaa tcccaaagga ctcaagcgtg atactggcaa tttcattggt tgctgcatat
      421 ggatgagccc gcggtgcagt cggaagctgc agttcaggac tgcgcgcgcg cgctgcctga
      481 ggatccgctc gaggaactga tagagatgcg ctaccagcag tacctcgctc agcgtgcgct
      541 ggaggaactg ttcgaaaatg gagcccttca ggcactcgag ggttgatacc ggccccgtaa
      601 gctccgcgat gttgcgagca tgagttgccg acgcgcttgc tcaacgcggc atcgccttga
      661 agggcggtga gcggatcgct tcaccggcag ccgggctggt cgctcttctc caaataatag
      721 gtgtacgtct cgcagtcacc ggccgtcggc gggcgcggga ggggaactgc acggtccacc
      781 gggggcagca atcgcacata agcaacgatc gcgcgtagat cctcctcgtc taaattggag
      841 agctggtccc acgtcatgcc ctgccaatgg agttgcctac cgtcccggct gacgccgctg
      901 cggatcgcgc gctctaactc catgtcgctc caggcgccga cgcccgcttg cggatgcgca
      961 gtgatgttgc gggtccacag cgtgccaaac ggcctccagc tgatcggcgt accgccgctg
     1021 ccgtcggtgc cgtggcagat ggcgcagctc actgagtaca agtagtggcc acgctccacc
     1081 atgcgaacgc gctcggcggg catgtcgctc gtgtccggct tgacatcgcc ggcgttcaca
     1141 gccttcacca gcggatcagg cggcatgttc cggatagcag gccactggga cagaaccaac
     1201 agcagcacac cagcgaccgc gaacaccagc acgcccccga tcgacgccac gatccctgga
     1261 cgcgggcccg cgcgcacgaa gagcaccagc gccagtatca gtattatcca ctgaacatcg
     1321 acgagcacag cttgaacacg cgccggcgag cccctggtgg aatccacggc gaaattgcca
     1381 tcgccatagg tcagtgcctt gggagcggtg gccggcagcg ggcgcgtgaa cttcatcgcg
     1441 atggtctcca ccagtccata ggcaagcttc ggcgggatcg ggttatgcac cggccgcgat
     1501 gccgtcttga ggaaggtggc gatggccgtt gcgtcctcgt cggtgaagtt gtggaactgg
     1561 gcccaaagca ttgcaaacgg gttgagggcc cggtctggcg cctggccgtc acgtagcgcg
     1621 cgaatgatct gcggcacagt ccagtgaccc aagcccgttt caggatccga agtgagattg
     1681 cggctgaccg agtagccgtg cgggtagtat ccgacgcgca ttccaccagc gaggaacgcg
     1741 ccgtcaacgt tgtagattcc ggtgcggtcg gtctgcgtat ggcagaggcc gcacacttcc
     1801 cagcgggtaa tgtactcgcc gcgctgcgct ggattcgcgg caaatcccgc accgccgaag
     1861 ctgccgtcct tctcccacgc cgccgtgcgg gcgatcgaag cgacgaaatt gaccaggtcc
     1921 caccgggcgg cggccgaaag caggtccgcg taggcgggca taggccccgg catgatcccg
     1981 gtagttagcc gcagccaaat gtcctggggc cgactgccgc cacggaaagc ccagggcgc
//
LOCUS       DATMRM010000481         5168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_254210, whole genome shotgun sequence.
ACCESSION   DATMRM010000481 DATMRM010000000
VERSION     DATMRM010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254210"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VNZ06_14635"
     CDS             complement(<1..826)
                     /locus_tag="VNZ06_14635"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWW22033.1"
                     /translation="MAEIRDAFEAYASGELAEYELRSALRAEIQTQPGSVHRYAAMAA
                     ALRRRNLISAELEAAVVSDMNEVVAQSRPIPAANSHEAPTRASMREPPPLEPAPLEPP
                     IAHAAEISHPSRPVAASTGAGGSASGIGRSASRGGAWDSKERLAEPETPVSVGMVLRE
                     RFELIEELGRGGMGVVYKAVDQREIENKGRDPYVAIKVLNEEFKRHPESARALQRESK
                     KAMRLAHPNIVLVRDFDRDRGNVYMVMELLHGTPLDQLVLKQHPKGMPLDQVIEIVN"
     gene            complement(1155..2228)
                     /locus_tag="VNZ06_14640"
     CDS             complement(1155..2228)
                     /locus_tag="VNZ06_14640"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWW22034.1"
                     /translation="MLVLLVSVAWCQESAHWPTQDGVFTLSNFRFGSGETLPQLRLHY
                     LTLGNLHRDKNGHTDNVVLLLHGTGGDAHSLLNPIFADVLFGPGQPLDINQYFIILPD
                     DIGHGSSSKPSDGLHAHFPKYDYDDMVKTQHTLLTQALKVDHLRLILGTSMGCMQSFV
                     WGETYPGFADALAPFACLPTEIAGRNRMMRYMAVQAIQQDPQWMGGDYEAEPVQGLRT
                     ANEMTLIMGSSALQMQKQAPTRAAAESYVDGYLKARLAATDANDLIYQFQASRNYNPS
                     EHLDRITVPVLYLNSADDYINPPELGIAQTLSKKMPQCRFVLIPISDATRGHGTHTVA
                     VVWKDYLVQLLKDSEPGARAVSH"
     gene            2482..4464
                     /locus_tag="VNZ06_14645"
     CDS             2482..4464
                     /locus_tag="VNZ06_14645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HWW22035.1"
                     /translation="MSMLRAERELTVRGLILGVVITLVFTAANVFFGLKAGLTFATSI
                     PAAVISMAVLRGFKNMTIQENNIVQTVASAAGTLSSIIFVLPGLVIIGWWTGFPYWTS
                     VLICGLGGILGVMYSIPLRRALVTQSDLPYPEGVACAEVLKVGGGAEAEASAIEDARA
                     GLLAVTWGSIVSGVYALIVATRIVAGDLVRTFRVGDRGAVSGFDLLFSFALFGIGHLV
                     GLWVGVAMLVGALIGWGWGVPHYSLLSPELTSVVPATLAQHTWSHQVRFVGAGTILVA
                     ALWTLAKLIRPVYGGLLTAMAASRARRAGNAAALPRTEQDIPIGLVGVLTLVCLAPIG
                     LLLSRFDSGGALNQHIALLAIGGLIFIAVMSFFVSSVCGYMAGLIGSSNSPLSGIGIL
                     AVIGASLLLLFGVRPNLTASADQALVAFALFVTAVVFSVACIANNNLQDLKTGQLVDA
                     TPWKQQVALVIGVIAGAVIIPPILDLLNHAYGFAGAVGAHREHALPAPQAGLISALAQ
                     GVLQHNVDWSLIEIGVGIGVALIVVDQVLRRGTHDKAHLSPLAVGLGIYLPTQSTLMV
                     VVGAVAGWYFDRRANRRANPASIKQLGVLLASGLIVGEGLIGVLIAALVAFSGKDFPL
                     SLVGDQFADNGAVVVGTVAFALICIGMYRWVAGLRK"
     gene            complement(5033..5158)
                     /locus_tag="VNZ06_14650"
     CDS             complement(5033..5158)
                     /locus_tag="VNZ06_14650"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWW22036.1"
                     /translation="MINTLSHDPFAPFGGFKQSGIGREGGIFGLQEYLEPKAIIG"
BASE COUNT          929 a         1538 c         1619 g         1082 t
ORIGIN      
        1 cgttgacgat ctcgatcacc tggtcgagcg gcatgccctt cgggtgctgc ttgagcacca
       61 gctggtcgag cggcgtgcca tgcagcagct ccatgaccat atagacattg ccgcgatcgc
      121 gatcgaaatc gcggaccagc acgatgttcg gatgcgccag acgcatcgct ttcttggact
      181 cgcgttgcaa cgcgcgcgcc gactccggat ggcgcttgaa ctcctcgttc agtaccttga
      241 tcgcgacgta cggatcgcga cccttgttct cgatctcgcg ctgatcgacg gccttgtaaa
      301 caacgcccat gccgccacgg ccgagctcct cgatcagctc gaagcgctcg cgcaacacca
      361 tacccaccga caccggtgtt tccggttcgg ccaatcgctc cttgctgtcc catgcaccgc
      421 cgcggctggc gctgcggccg atgccagacg ccgatccgcc agctccggtc gatgcggcca
      481 ccggacgtga cgggtgagag atctctgcgg cgtgcgcgat gggaggctcg agaggcgcag
      541 gctccaacgg cggcggctcg cgcatcgacg ctcgggtcgg cgcttcgtgg ctgttggcgg
      601 ccgggatcgg cctggactgc gcgacgacct cgttcatgtc cgagaccacc gccgcttcca
      661 gttcggcact gatcagattg cggcgacgca gcgccgcggc catcgcggca tagcgatgca
      721 cggatcccgg ctgcgtttgg atctcggcgc gcagagcgct gcgaagctcg tactcggcaa
      781 gctcaccgga cgcgtaggcc tcaaacgcat ccctgatctc ggccatgccc atctgaaaat
      841 ccctctgtcg atcaatctac tacgatcgaa tagttatcaa cttcattgga ttcggcgtga
      901 ctggcgctgt cgttcgcgag tcagcctgaa gcggatttga ggatgttagc tcaccgtccg
      961 ccggatacca gtatgcccac gatgttcaac ggaaaaaaat acatgctgat aaaaaacggt
     1021 gcagccgtcg ctccagtggt cattgtgttg ctgtgtattc ctcagcaaca cagttcactc
     1081 agtgcgccaa ctctatgaaa agcattgtgt cgttgccggc cgcggcgtcg gggagtgcgt
     1141 cggccttgac gcgcctagtg gctcacagcg cgagcgccgg gctccgaatc cttcagaagc
     1201 tgcaccagat agtccttcca gacaacggct accgtatgag tgccgtgacc acgcgtcgca
     1261 tcgctgatgg gaatcaggac gaaacgacac tgaggcatct tctttgacag ggtctgcgca
     1321 atccccaact ccggcggatt gatgtagtcg tcagcagaat tgaggtacag caccggcacc
     1381 gtaatgcgat ccagatgctc gctggggttg tagtttcggc tggcctggaa ctggtagatc
     1441 agatcgttcg cgtcagtggc agcgagtcgg gctttcagat agccatccac gtagctctcg
     1501 gctgccgcgc gtgtcggagc ctgcttctgc atctgtagcg cactggagcc catgatcagc
     1561 gtcatctcat tcgcggtgcg cagaccctgc accggctcgg cttcatagtc accgcccatc
     1621 cactgcggat cctgctggat cgcctgtacc gccatgtaac gcatcatgcg gttgcggccc
     1681 gcgatctcgg tgggcagaca ggcaaatggt gcgagcgcgt cagcgaagcc cgggtacgtc
     1741 tcgccccaga cgaaagactg catgcagccc atcgatgtac caagaatcag ccgcagatga
     1801 tcgaccttga gagcttgcgt gagcagggtg tgctgagtct tcaccatgtc atcgtagtcg
     1861 tactttggga aatgcgcgtg cagcccatcg gagggtttgg acgatgagcc gtgaccgatg
     1921 tcatccggca ggatgatgaa gtattggttg atgtcgagcg gctgtccagg tccaaacagg
     1981 acgtcggcga agatgggatt gagcagcgaa tgcgcatccc cgccggtgcc gtgcagcaac
     2041 agcacgacat tatccgtgtg gccgttcttg tcccggtgca agttgccaag cgtcagatag
     2101 tgcagtcgca gctgcggcag cgtctcgccg ctcccgaacc ggaagttgga aagcgtgaac
     2161 accccatcct gcgtcggcca atgcgcgctt tcctggcacc acgccaccga gaccagcagg
     2221 acgagcattg cgacggaaag caagcgcgaa cacatgtgag ggtctcctga ggcgactgag
     2281 gtggaacgca ttctggggga cgcggtcgaa cttgggcaaa tgtgaggcag ccaagtagcg
     2341 ctgctgtcaa agggccagcg gcaccagcgc aacgcgcgtg ccgctgcacg tggtgaaagt
     2401 gcgctatcct gcacgccgcg caaggcaggc gaccgttacc gggtccaata agaatagtat
     2461 ttccggggga ggagcgcatt catgagcatg ttgagggcag agcgagaact aaccgtacgc
     2521 ggactgattc tgggtgttgt cattaccctg gtgttcacgg ccgcgaacgt gttctttggc
     2581 ctcaaggcgg gcctgacctt tgcgacctcg atcccggccg cggtcatctc gatggcggtg
     2641 ctgcgcggat tcaagaacat gaccatccag gagaacaaca ttgtgcagac ggtcgcgtca
     2701 gcggccggta cgttgtcctc gatcatcttc gtgctgccgg gcctggtgat catcggctgg
     2761 tggacgggct ttccgtactg gacgtccgtt ctgatctgcg gattgggcgg gattctgggc
     2821 gtcatgtatt cgatcccgtt gcggcgcgca ctggtgactc agtcggatct gccgtatccg
     2881 gaaggcgtcg cgtgcgcgga agtactgaaa gtcggcggcg gcgccgaggc tgaagcctcc
     2941 gcgatcgaag acgcgcgggc cggcctgctt gcggtgactt ggggctctat cgtttccggc
     3001 gtctacgcac tgatcgtcgc gacccgcatt gtcgccggcg atctggtgcg cacgttccgc
     3061 gtcggcgatc gcggtgcggt gagcggcttc gatctgttgt tctcgtttgc attgttcggc
     3121 atcggtcatc tggtcggact gtgggtgggt gtggcaatgc tggtcggcgc actcatcggc
     3181 tggggctggg gtgtgccgca ctactcgctg ttgagccccg agctgacctc ggtcgttccg
     3241 gcaacgctgg cgcaacacac gtggagtcat caggtgcgtt tcgtcggtgc cggcaccatt
     3301 ctggtcgcag cgctctggac gctggcaaag ctgatcagac cggtttacgg cggattgctc
     3361 accgcgatgg ccgcatcgcg cgcgcgccgc gccggcaatg ccgccgcgct gccacgcacc
     3421 gagcaggata ttccgatcgg actggtgggt gtgctgacgc tggtgtgtct cgcaccgatc
     3481 ggcctgttgc tttcgagatt cgattcgggc ggcgcattga atcagcacat cgcgctgctg
     3541 gcgattggcg gcctcatctt cattgcggtc atgagcttct ttgtctcgtc ggtctgcggt
     3601 tacatggccg gtctcatcgg ctcatccaac agcccgttgt ccggcatcgg cattctggcg
     3661 gtgatcggcg catcattgct gttgctgttc ggagtgcgtc ccaacctcac tgcgagcgcg
     3721 gatcaggccc tggtcgcgtt cgcattgttt gtgaccgcag tggtgttctc ggtcgcctgt
     3781 atcgccaaca acaacctgca ggacctgaag accgggcagt tggtggatgc gacaccgtgg
     3841 aaacaacagg tcgcgctggt cattggtgtc atcgccggcg cggtcatcat tccgccgatt
     3901 ctcgatctgc tcaatcacgc ttatggattt gccggggccg ttggtgcgca ccgcgaacac
     3961 gcgctgcccg caccacaggc gggtctgatc tcggcgctgg cgcagggcgt gctacagcac
     4021 aacgtggact ggagcctgat cgagatcggc gtcggcatcg gcgtggcgct gatcgttgtc
     4081 gaccaggtgc ttcgtcgcgg tacccacgac aaggcgcatc tgtccccgct ggcagtgggc
     4141 ttggggatct acctgccgac acagagcaca ctgatggtcg tggttggcgc cgtggccggc
     4201 tggtatttcg atcgacgcgc gaatcgccgc gccaatccgg ccagcatcaa acagctcggc
     4261 gtgctgctgg cttcgggcct gatcgtcggc gaaggcctga tcggtgtgtt gatcgcggcg
     4321 ctggtcgcct tctccggcaa ggactttccg ttgtcgctgg tcggcgatca atttgccgac
     4381 aacggcgcgg tcgtagtcgg aacggtcgcg ttcgcgctga tctgcatcgg catgtatcgc
     4441 tgggtcgcgg gactgcgcaa gtgagcgctg acgacccacc cagagatgcc gacaattgct
     4501 acactgcgtc cggcgtgggc ctgtagcaca acggttagtg caggagactc atctgaagag
     4561 gtgccttcac ggtgaaaatc gtgaagtgga cgggatgaat tcagggaaac cttccggtcg
     4621 catgaccgat ggcaatcctg agccaagcca gtcgtaaacg actggaaggt gcagagacta
     4681 cctgagagct gcggattcga gttcgcgatg gctcttgata acaggcgtag agcgtcccgc
     4741 gcccatgatg caggttggac tcgcatcatc gggtgaagag atagtccact cctatcggaa
     4801 acgatggggc aaagtgaatc tcttggttgc aggttcgaat cctgccgggc ccaccacttc
     4861 cctcggccgg ggaacaccgg aaccacaaac tgtcggcgaa cgacacttct gccggtttcg
     4921 gccaaaggat gtctaaaccg cgctgccggt gactaaccat actttgttca taaatttccg
     4981 cctttcttct ttgcacttat ttcccggtat cagaacgtcg gacatttctc cgctatccga
     5041 tgatcgcttt cggttccagg tactcctgca gcccgaagat cccgccttcc cgaccgatcc
     5101 ccgattgttt gaacccaccg aagggagcaa acggatcgtg acttaatgtg ttgatcagga
     5161 cgcggccg
//
LOCUS       DATMRM010000482         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2447892, whole genome shotgun sequence.
ACCESSION   DATMRM010000482 DATMRM010000000
VERSION     DATMRM010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2447892"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="VNZ06_14655"
     CDS             <1..1331
                     /locus_tag="VNZ06_14655"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22037.1"
                     /translation="QTNLRRSSIEAERGEVMLDITQAQESLARARSAHGAAIEALARL
                     DAQKPELENERELIRERVGLARTRAQAAQTELHDSRIALESRRSAQGSMANALGRMLE
                     QRSSLETQRAGLERTLADGDAPIEQLQTRLQDHLVRRAEVETELATARHAVEEGEVEL
                     RALEQRRLAAEQRVMAGREAMEQARLAAQESRVRRESLLEQFVATHCELQAVLENLHP
                     EAAILTWETRLNDLRVDIEKLGQVNLAAIDELAEQSERKSYLDRQFTDVNDALNTLDQ
                     AMRKIDRETRTRFEDTFNRINAGLQEKFPRLFGGGHAYLELVGEDPLAAGVAVMARPP
                     GKRNSSISQLSGGEKALTAVALVFSIFDLNPAPFCLLDEVDAPLDEHNVGRFCDIVRD
                     MSSRVQFIFITHNKVTMEMAQQLIGVTMNEPGVSRLVAVDIDEAVKLAAV"
     gene            1346..>2162
                     /locus_tag="VNZ06_14660"
     CDS             1346..>2162
                     /locus_tag="VNZ06_14660"
                     /inference="COORDINATES: protein motif:HMM:NF016257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HWW22038.1"
                     /translation="MAELRWILLLAGVVFLAGLTAWELRRPRQARRDTLGPPERSEPP
                     LGAMHEFEADSPAARVSPSVQTEVTPFGSPLVISLDAPVEPPTLHPLPALHAIEPAAP
                     LERADPPQSCAETAESAQRAVPDEDPLPSGMRPGEIVVPVLLAAEPVDPIVEWPPEDQ
                     RYILSVRVVGVNPQRLMGRPLRQALAACGFVHGRFGIFHQPGADGRALISAANLSKPG
                     VFDPNSMDFQRFRGLGLFAVLPGPLPPAAALDHLLDTAGDLAERLQARLQDEHG"
BASE COUNT          378 a          677 c          754 g          353 t
ORIGIN      
        1 agcagaccaa ccttcgccgc tcgagcatcg aagccgagcg cggcgaggtg atgctggaca
       61 tcacgcaggc gcaggagagc ctcgcgcgcg cgcgctcggc gcacggcgcg gcgattgaag
      121 ccctcgcgcg tctggatgcg cagaaacctg aactggagaa cgagcgcgaa ctgatccgcg
      181 agcgtgtcgg cctcgcgcgc acgcgtgctc aggccgccca gaccgagctg cacgacagcc
      241 gcatcgcgct cgaaagccgg cgctcggcgc agggctcgat ggccaatgcc cttggccgca
      301 tgctcgagca gcgcagctcg ctcgaaacac agcgtgctgg cctcgaacgc acgctggccg
      361 atggcgatgc gccgatcgag cagctgcaga cgcgtctgca ggatcacctg gtgcggcgtg
      421 cggaagtgga aaccgaactg gcgaccgcac ggcacgcggt ggaagagggc gaggtcgaat
      481 tacgcgcgct ggaacagcgc cgcttggctg ccgagcagcg cgtgatggcc ggacgcgagg
      541 cgatggagca ggcgcgtctg gcggcgcagg aatcgcgcgt gcggcgtgaa tcactgctcg
      601 agcagttcgt cgcgacgcac tgcgagctgc aggcggtgct cgagaatttg catccggaag
      661 cggcgatcct cacctgggag acgcgactga acgacctgcg ggtcgatatc gagaagctcg
      721 ggcaggtgaa tctcgccgcg atcgatgagc ttgccgagca aagcgagcgc aagagctacc
      781 tggaccgtca gttcaccgac gtcaacgatg ccctcaacac gctcgatcag gcgatgcgca
      841 agatcgaccg cgagacgcgc acgcgcttcg aggatacgtt caatcgcatc aacgccggtc
      901 tgcaggaaaa attcccgcga ctcttcggcg gcggccatgc ctacctggaa ctggtgggcg
      961 aggatccgct cgcggccggc gtcgccgtca tggcgcgtcc gcctggcaag cgcaacagtt
     1021 cgatttcgca gctgtccggc ggtgaaaagg cgctgaccgc ggtggcgctg gtgttctcga
     1081 tcttcgatct gaatcctgcg ccgttctgcc tgctggatga ggtggacgca ccgctggatg
     1141 aacacaacgt cggccggttt tgcgatatcg ttcgcgatat gtcgagtcgt gtgcagttta
     1201 ttttcatcac gcacaacaaa gtgacaatgg aaatggccca acagctcatt ggggtgacca
     1261 tgaatgagcc tggcgtgtcg cggttggtgg cggtcgatat cgacgaggcc gtgaagctcg
     1321 ccgcggtctg accgcgaagt caaccatggc ggagctgcgc tggattttgt tgctggccgg
     1381 agtggtgttt ctggcgggcc tgaccgcgtg ggaactgcgt cgtccgagac aggcaagacg
     1441 cgatacgctc ggaccgcccg agcgcagcga accgccgttg ggtgcgatgc acgagttcga
     1501 ggccgactcc cctgccgccc gggtatcccc gtcggtgcaa acggaagtca cgccgttcgg
     1561 ctcgccgctg gtcatctcac tggacgcgcc ggtggagccg ccgacccttc atccgctccc
     1621 cgcattgcac gcgatcgaac cggcagcccc gctcgagcgc gcggatcccc cgcagagctg
     1681 cgccgagacg gccgagagcg cgcagcgggc agtgccggat gaggatccat tgccgagcgg
     1741 catgcggccc ggtgagatcg tggtaccggt gttgctggcc gccgaaccgg tcgatccgat
     1801 cgtggagtgg ccgccggagg atcaacgcta cattctttcg gtgcgcgtgg tgggggtcaa
     1861 tccgcagcgc ctgatgggca gaccgctgcg tcaggcgctg gcggcctgcg gcttcgtgca
     1921 cgggcgcttc ggaattttcc atcagcccgg agcggacgga cgcgcgctga tcagcgccgc
     1981 gaacttgagc aagccgggtg tattcgatcc caacagcatg gacttccagc gcttccgcgg
     2041 actgggactg ttcgcggtgt tacccggtcc gttgccgccg gcggcggcgc tggatcatct
     2101 gctggatacc gcaggggacc tggccgagcg cttgcaggcg cgtctgcagg acgagcacgg
     2161 tc
//
LOCUS       DATMRM010000483         8026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3056734, whole genome shotgun sequence.
ACCESSION   DATMRM010000483 DATMRM010000000
VERSION     DATMRM010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3056734"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1190)
                     /gene="epmB"
                     /locus_tag="VNZ06_14665"
     CDS             complement(129..1190)
                     /gene="epmB"
                     /locus_tag="VNZ06_14665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HWW22039.1"
                     /translation="MITTSPLSRQPEAGRPKAVWQRELAQAITDPLELCRALGLDPEL
                     ALGARAALSHFPLRVPRGFLRRMRHGDPADPLLRQVLPLPSEAMTTAGFNADPLIERG
                     AMRAPGLLHKYPGRALLITTEACAVHCRYCFRREFPYQSAHSEGGRWREALEVIAQDD
                     SIHELILSGGDPLSLGNDRLSGLTAQLRHIPHLRRLRLHTRTPIVLPERVDDELLAWL
                     RALPWPCVIVLHCNHANEIDAGVREATALLKSAGAVLLNQSVLLKGVNDSADALCHLS
                     EGLWSAGVLPYYLHLLDRVTGTAHFEIGEWRARELMRQVSGRLPGYLVPRLARECPGA
                     VSKTVLAPGWDALHSLPQP"
     gene            1244..1813
                     /gene="efp"
                     /locus_tag="VNZ06_14670"
     CDS             1244..1813
                     /gene="efp"
                     /locus_tag="VNZ06_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HWW22040.1"
                     /translation="MATYSTSEFKSGLRIIIDGDPFAIVENEMVKPGKGQAFNRVRIR
                     NLKTGRTIERTFKSGDSVEAADVSDVDMQYLYSDGDFFHFMIPENFEQHAATKAVVGE
                     SAQWLKDGVVCIVTLWNGVPIVVTPPPHIELKVVETDPGLRGDTATGGQKVAKLETGA
                     VVRVPLFINEGEILKIDTRTGAYISRAKQ"
     gene            complement(1837..2799)
                     /gene="epmA"
                     /locus_tag="VNZ06_14675"
     CDS             complement(1837..2799)
                     /gene="epmA"
                     /locus_tag="VNZ06_14675"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HWW22041.1"
                     /translation="MNWRPSATPATLKLRADALRAVREFFAERQVMEVDTPALVRHAV
                     TDLNIHSARVSLPGHHEPLYLHTSPEYAMKRLLAAGSGDIYQISHVYRGDESSRLHNA
                     EFTLIEWYRCGFGMRQLMREVAALVGCLLGNSGSDAFTELSYAQAFRQSVGCDPLAAD
                     ESQLRSLAIAHRLDPPMAARCSRDELLDWLMGSVIGPALGADGLCFVHRYPVSQAALA
                     RVDPADPALALRFELYRHGIELANGFEELANPVEQRARFESDRNERSRRGLPVPEIDQ
                     SLLDALAAGLPPVSGVALGFDRTLMLRIGARAISEVLPFALDRA"
     gene            complement(2796..3686)
                     /gene="asd"
                     /locus_tag="VNZ06_14680"
     CDS             complement(2796..3686)
                     /gene="asd"
                     /locus_tag="VNZ06_14680"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HWW22042.1"
                     /translation="MSRLFVTLQYLLPHHLLGRLIYALTRVRAPPVKNWLIRTFCRLF
                     RPEMSDAVQSDPLRYATFNAFFTRALKPDARPLDPHPRRILSPCDGTLSVAGTLSEGR
                     MLQAKGHWYTPAALLAAPLESAAPLESAALHDGRYATLYLAPYNYHRLHMPLSGRLRA
                     AWHVPGRVFSVSARTVARVPGLFARNERVVCVFDGEHGAFVLVLVGALFVGSMSTVWH
                     GDITPWRGPARRPSDPAGIHPLAPSSGADLWRARGAEFARFNMGSTIVMLMPAAMGAW
                     DASLSPGERVAVGQGLGTLT"
     gene            complement(3683..4297)
                     /locus_tag="VNZ06_14685"
     CDS             complement(3683..4297)
                     /locus_tag="VNZ06_14685"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HWW22043.1"
                     /translation="MNRRLALVFALTAVAAALAGVVAARLVGGHGGVPLVSGTWLPSR
                     RPVAEFHLEDLSGQGFTLQNLRGHPTLLFFGFTHCPDVCPITLATLAQAQRTVPLPGA
                     QVVFISIDPQRDSAANMSEYLSAFSKDFIGARGDEAALGPLLKSLGAIAERERLPDGN
                     YTLDHSATLYLLDGQARLAAVFSPPFKTADLTADLRRIATDASL"
     gene            4313..5167
                     /gene="prmB"
                     /locus_tag="VNZ06_14690"
     CDS             4313..5167
                     /gene="prmB"
                     /locus_tag="VNZ06_14690"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: protein motif:HMM:TIGR03533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWW22044.1"
                     /translation="MGQLLELGRGRLARARLHFGHGTDNAHDDAAELLRFALRLPSGV
                     SVKLSRRVSGARQRLYQSLLERRIRERIPVVYLTGECWFAGLRMQVDPRVLIPRSAIA
                     ELIERRFQPWVRPQKVRRILDLGTGSGCIAIACARAFPRATVDASDISSDALAVARIN
                     IRRHRLSKRVHALRSNHFAGLGARSYDIIVSNPPYVGRREFDALPAEYRHEPRRALEA
                     GTDGLDSVRVILRGAARRLRPGGVLIVEVGNSQTAVRRVFRKLPFLWLTFERGGGGVF
                     LLEREQLL"
     gene            5190..6329
                     /gene="aroC"
                     /locus_tag="VNZ06_14695"
     CDS             5190..6329
                     /gene="aroC"
                     /locus_tag="VNZ06_14695"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HWW22045.1"
                     /translation="MSGNTIGRLFSVTTFGESHGAALGCIVDGCPPGMALQEADLQPD
                     IDRRRTGTSHFVSQRREADIVRILSGVFEGRTTGTPIGLLIENTDARSRDYDKLKDRF
                     RPGHADYTYVHKYGMRDHRGGGRSSARETVMRVAAGAIARKYLAERLQVRIYGYLSQI
                     GSLKLEPRAPAAAYNNPFYCPDPARVPELEQLMWSIRGTGDSVGARITVCAEGVPPGW
                     GEPVFEKLDADLASAMMGINAVKAVEIGDGVAVVSQRGSEHRDELTPIGFASNHSGGT
                     LGGISTGQPIVVHLSLKPTSSIQVPGRTIDLAGNPVDVVTTGRHDPCVGLRATPIAEA
                     MLAIVLMDHYLRHRGQNADVVCAVPDIEARGREQVPHASGETDKR"
     gene            6326..7351
                     /locus_tag="VNZ06_14700"
     CDS             6326..7351
                     /locus_tag="VNZ06_14700"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWW22046.1"
                     /translation="MSRSFTVAVVGATGLVGDSMISILEERQFPVSELHALASNRSLG
                     KGVQFHGRTLPVEELDSFDFARADIGLFSAGAEVSAQFAPKAAAAGCIVIDNTSQFRY
                     QDDIPLVVPEINPHAIGQYRNHGIIANPNCSTIQMLVALKPIYDAVGIERINVATYQS
                     VSGAGKEAIEELAAQTRQLLNGRPIESPRVVARQIAFNCVPQIDKFQDNGYTKEEMKM
                     VWETQKIMEDKNIRVNATAVRVPVFYGHSEAVHIETSRKISADEARALLQKAPGVTVL
                     DERQPGGYPTAVTEAANHDTVYVGRIREDMSHERGLDLWIVADNIRKGAATNSIQIAE
                     ILVREYL"
     gene            7449..>8026
                     /locus_tag="VNZ06_14705"
     CDS             7449..>8026
                     /locus_tag="VNZ06_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22047.1"
                     /translation="MFKRNLCQLLLLALLSPAATFALGLGEIHLKSALNAPLDAEIDV
                     VGASPEDLAGLKARLAPRDAFVREGLDYPSYLNTLSVSAEKTSDGRNVLHLHTGDPVS
                     EPFATLLVEADWPRGHVVREYTVLIDPPVFSAQNATNAAVAAPATGATVRSGAVERPA
                     ERASAGSTSSANTSAPSAAGSTSGGRAGAAAAG"
BASE COUNT         1499 a         2539 c         2631 g         1357 t
ORIGIN      
        1 cggcgatccc ggtccggcgc agcaaaccgt tcagagcctc gacggcatcg cgagtggcac
       61 tgtgtacgat cagcggaacc gctgtggtgt cgttcaactg ctgtctccgg aaccctgaaa
      121 ttctagcatc agggctgtgg gaggctgtga agcgcatccc agccaggggc caatactgtc
      181 ttcgagacgg ccccggggca ctcacgggcc agtcgtggca ccaaataacc cggcagccgg
      241 ccgctcacct gccgcatcag ttcgcgcgcg cgccactcac cgatctcaaa gtgtgcagtg
      301 cccgtcaccc ggtcgagcag atgcaggtaa tagggcagta caccggcaga ccacaggcct
      361 tccgagagat gacacagcgc gtcggccgaa tcgttgacac ccttgagcag cactgactga
      421 ttcagcaata cagcaccggc actcttgagc aacgcggtgg cctcgcgcac gccggcatcg
      481 atttcattcg catgattgca gtgaagcact atcacacacg gccagggcag cgcacgcaac
      541 cacgccagca gttcatcgtc gacgcgctcg ggcagtacga tcggcgtgcg ggtatgcagg
      601 cgcaggcgcc gcagatgcgg gatatgacgc aactgcgcgg tcagcccgga cagccgatca
      661 ttgccgagcg acagcggatc gccacccgag aggatcagct catggatgga atcgtcttgc
      721 gcgatgacct cgagcgcttc gcgccagcgg ccgccctcgc tgtgcgcgct ctgataggga
      781 aactcacgcc ggaaacagta gcggcaatgt accgcgcagg cctcggtcgt gatcagcagc
      841 gcccggcccg ggtatttgtg caacaggccc ggcgcgcgca tggcgcctcg ttcgatgaga
      901 gggtcggcat tgaagccggc agtcgtcatc gcctccgatg gcaacggcag cacctgccgc
      961 agcaacgggt cggctggatc cccgtggcgc atgcgccgca ggaaacctcg cggcacccgc
     1021 agcgggaagt gtgacagggc ggcgcgtgcc ccgagcgcca gttcagggtc cagccccagt
     1081 gcacggcaca gctcgagcgg atcggtaatg gcctgcgcca gctcacgctg ccatacggct
     1141 ttcggccggc cggcctctgg ctggcggctg agaggtgatg tcgttatcat acgcgccctt
     1201 ttttgacgac cggcattgtg cggccgtaac cggcggaaat gaaatggcta cttacagcac
     1261 cagtgagttc aagtcgggcc tgcggatcat catcgatggc gatccgtttg ccatcgttga
     1321 gaacgagatg gtcaagccgg gcaagggcca ggccttcaat cgcgtgcgca tccgcaacct
     1381 gaaaaccggg cgcacgatcg agcgcacgtt caagtcgggc gatagcgtcg aggctgcgga
     1441 tgtgtccgac gttgacatgc agtatctcta cagcgacggc gatttcttcc atttcatgat
     1501 cccggagaac ttcgagcagc atgcggcgac caaggccgtg gtcggcgaaa gcgctcaatg
     1561 gctcaaggac ggagtggtct gcatcgtcac actgtggaac ggtgtgccga tcgtcgtgac
     1621 cccgccaccg cacatcgaac tgaaagtcgt cgagaccgat cccgggctgc gcggcgatac
     1681 cgccaccggc ggacagaagg tcgccaagct cgagaccggc gcggtggtgc gggtgccgct
     1741 gttcatcaat gaaggcgaaa ttctgaagat cgacacccgc acgggtgcct atatctcgcg
     1801 cgccaagcag tagtgcgaag ttcggcgccg gagctctcag gcgcgatcga gcgcgaaggg
     1861 cagcacctca ctgatcgcgc gcgcaccgat ccgcagcatc agcgtgcggt cgaatcccag
     1921 tgcgacgcca ctgacgggtg gcagacccgc ggccagtgcg tccagcagtg actgatcgat
     1981 ctcgggcacc ggcagaccgc ggcggctacg ctcgttgcga tccgactcga aacgtgcccg
     2041 ctgttcgacc ggattggcca gttcttcgaa gccattggca agctcgatgc cgtggcggta
     2101 cagctcgaaa cgcagcgcca gcgcgggatc ggccggatcg acccgcgcga gcgcggcctg
     2161 gctcaccgga tagcgatgca cgaagcacaa cccgtcggcc ccaagcgccg gaccgatgac
     2221 actacccatc agccagtcga gcagttcgtc ccgactgcag cgcgcagcca tcggcgggtc
     2281 gagtcgatgc gcgatcgcca gcgagcgcaa ttgcgattcg tcggccgcca gcgggtcgca
     2341 gccgacggat tgacggaagg cctgcgcgta gctcagctcg gtgaacgcat cgctgccgct
     2401 gtttcccagc aggcacccca ccagagcggc cacttcccgc atcaactggc gcatcccaaa
     2461 gccgcagcga taccactcga tcagcgtgaa ctccgcattg tgcagacggc tgctctcgtc
     2521 accgcgatag acgtgcgaaa tctgatagat gtcgccgctg cccgcggcca gaagacgctt
     2581 catcgcatat tcgggcgagg tgtgcaggta cagcggttcg tggtggcccg gcagtgagac
     2641 ccgcgcgctg tgaatgttga ggtcagtcac cgcgtggcgc accagcgctg gcgtgtccac
     2701 ctccatcacc tgccgttcgg cgaagaactc acgcacggcg cgcagcgcat ccgcccgaag
     2761 cttcagggtg gccggcgtcg cggacggacg ccagttcacg tcagcgttcc caatccctgg
     2821 ccgacggcga cccgctcgcc gggtgacagc gacgcatccc aggcgcccat ggcggccggc
     2881 atcagcatca cgatggtcga tcccatgttg aagcgggcga attccgcgcc gcgggcccgc
     2941 cacaggtcgg caccgctgct cggcgcgagc ggatgaatcc ccgccgggtc cgacggccgc
     3001 cgcgccgggc ctcgccacgg ggtgatatcg ccatgccaca ccgtgctcat gctgccgacg
     3061 aacagggctc cgaccagcac cagcacgaac gctccgtgct cgccatcgaa cacacacacg
     3121 acccgctcgt tgcgggcaaa cagacccgga acccgcgcca ccgtgcgcgc gctcaccgag
     3181 aacacgcgcc ccggcacgtg ccaggcggca cgcaggcgac ccgacagcgg catgtgcaga
     3241 cgatgatagt tgtagggcgc cagatacagg gtggcgtagc ggccatcgtg cagcgccgcc
     3301 gattcgagcg gtgccgccga ttcgagcggt gccgccagca gggctgccgg cgtataccaa
     3361 tgccctttgg cctgcagcat gcgcccttcg ctcaaggtcc cggcaacgct cagcgtgcca
     3421 tcgcaggggg agaggattcg ccgcgggtgc ggatcgagcg gtcgggcatc gggtttgagc
     3481 gcgcgcgtga agaacgcgtt gaaggttgca tagcgcagcg gatcggattg caccgcgtcg
     3541 gacatctccg gtcgaaacag gcggcagaag gtgcggatga gccagttctt gaccggcggc
     3601 gcgcgcaccc gggtcagcgc atagatgagc cggcccagca gatgatgcgg cagcaggtac
     3661 tgcagggtaa cgaacaggcg actcacaggc tcgcgtcggt tgcgatgcga cgcaggtccg
     3721 cggtgagatc tgcggtcttg aacggcggcg agaacaccgc cgccagcctg gcctgcccgt
     3781 ccagcagata tagcgtcgcc gaatggtcca gcgtgtagtt gccgtccggc agtcgctcgc
     3841 gttccgcgat cgcgcccaga ctcttcagca gcgggcccag tgcggcctcg tcgccgcgtg
     3901 cgccgatgaa atccttgctg aaggcgctca gatactctga catattggca gcgctatcgc
     3961 gttgcggatc gatgctgatg aacacgacct gcgcacccgg cagcggcacc gtgcgctggg
     4021 cctgtgccag cgtcgccagc gtgatcggac atacatcggg acagtgggtg aagccgaaga
     4081 acagcagcgt cggatggccg cgcagattct gcagcgtgaa cccctgaccg gagagatcct
     4141 caagatgaaa ctcagccacc ggcctgcggc tcggaagcca ggtaccgctc accagcggca
     4201 cgccgccgtg gccgcccacg agccgggccg ccacgacacc ggccagcgcg gccgccacgg
     4261 ccgtcagggc aaacaccagc gccagtcgtc ggttcatggc ggcagtataa cggtggggca
     4321 gctgctcgag ctcggccgcg ggcgcctcgc gcgcgcgcgc ctgcacttcg gacatggcac
     4381 tgacaatgcg catgatgatg cggccgagct gctgcgcttc gcgctgcggc taccgtcggg
     4441 tgtgagcgtg aaactcagca ggcgcgtttc gggggcgcgc cagaggctgt accagtcgct
     4501 gctcgagcga cgcattcgtg agcgtatccc ggtggtctac ctgacaggtg agtgctggtt
     4561 tgccgggctg cgcatgcagg tggatccgcg tgtgctgatc ccgcgctctg cgattgcgga
     4621 gctgatcgag cgacgttttc agccctgggt acggccacag aaggtacggc gcattctgga
     4681 tctgggtacc ggttcgggct gcatcgcgat cgcctgcgcg cgggcgttcc cgcgtgccac
     4741 cgtggacgca tccgacattt cctcggatgc actggccgtg gcgcgcatca atatccggcg
     4801 gcaccgcttg tcgaagcgtg tgcatgcgtt gcgatccaat catttcgccg gactcggtgc
     4861 ccgcagctac gatatcatcg tcagcaatcc gccctatgtg gggcggcgcg aatttgatgc
     4921 tctgccggcc gaatatcggc atgagcctcg gcgtgcactg gaggcaggca cggacggctt
     4981 ggactcagtg agggtgattc tgcgcggggc agccagacgg ctgcggcccg gtggggtcct
     5041 gatcgtcgag gtaggtaact cgcagaccgc agtccggcgg gtgttccgca agctgccttt
     5101 cctttggctc accttcgaac gcgggggtgg tggcgttttc ttgctggagc gagagcagct
     5161 cctttagggc ctggtcgcga aacaattcaa tgtcgggcaa cacgatcgga agattgtttt
     5221 cagtcacgac ctttggcgaa agccacgggg cggcgctggg ctgcatcgtc gatggctgcc
     5281 ccccgggaat ggcgctgcag gaggctgatc tgcagcccga catcgatcgg cggcgcaccg
     5341 gcacttcaca tttcgtcagt cagcgacgcg aggccgacat cgtgcgcatc ctgtcgggtg
     5401 tgttcgaggg ccgcaccacc ggcaccccga tcgggctgct gatcgagaat accgacgcgc
     5461 gttcgcgcga ctacgacaag ctcaaggatc ggtttcgccc cggacatgcc gactacacct
     5521 acgtgcacaa gtacggcatg cgtgatcatc gcggcggcgg acgctcctcg gcgcgtgaga
     5581 ccgtgatgcg agtcgcggcc ggagcgattg cgcgcaagta tcttgccgag cgactgcagg
     5641 tccggatcta cggctacttg agccagatcg gctcgctcaa gctcgagccg cgagcgccgg
     5701 cagccgccta caataatcct ttctactgcc cggacccggc gcgcgtgccc gaactcgagc
     5761 agctgatgtg gtcgatccgc ggtaccggtg attcggtggg cgcacgcatc acggtgtgcg
     5821 ccgaaggtgt gcccccgggc tggggggagc cggtgttcga aaagctggat gccgatctgg
     5881 cctcggccat gatgggcatc aatgcggtga aagcggtgga gatcggcgat ggcgtcgcgg
     5941 tggtgtctca gcgcggcagc gaacatcgcg acgaactcac gccaatcgga tttgcatcca
     6001 atcattccgg cggcacgctc ggtggcatct ctaccggtca gccgatcgtg gtgcacctga
     6061 gtctgaaacc cacctcgagc attcaggtgc ctggccgcac catcgatctg gcgggcaatc
     6121 cggtcgatgt ggtgacgacc gggcgtcatg atccctgtgt gggactgcgc gcgacgccga
     6181 ttgccgaggc gatgctggcg atcgtgctga tggatcacta cctgcgccat cgggggcaga
     6241 acgcggatgt cgtgtgcgcg gtgcccgata tcgaggcgcg cggccgcgaa caggtgccgc
     6301 acgcatcagg agagacggac aagcgatgag caggagtttc acagtagcgg tggtcggcgc
     6361 gaccggtctg gtcggcgaca gcatgatcag tattctcgag gagcggcagt ttccggtgtc
     6421 ggaactgcat gcgctcgcca gcaaccgctc cctcggcaag ggcgtgcagt ttcatggccg
     6481 cacgctaccg gtagaggaac tggatagctt tgacttcgcg cgtgcggata tcgggctgtt
     6541 ctccgccggc gccgaagtct cggcgcagtt cgcgcccaag gccgcagccg ccggctgcat
     6601 cgtcatcgac aatacatcgc agtttcgcta ccaggacgac atcccgctgg tggtcccgga
     6661 gatcaacccg cacgcgatcg gccagtacag gaaccacggc atcatcgcga accccaactg
     6721 ttccactatc cagatgctgg tggcgctcaa acctatctac gatgcggtag ggatcgagcg
     6781 catcaatgtg gcaacctatc aatcggtgtc cggtgccggc aaagaggcta tcgaggagct
     6841 ggcagcgcag accaggcagc tgttgaacgg tcggccaatt gagtcaccgc gggtggtggc
     6901 acgccagatc gccttcaact gtgtaccgca gatcgacaag tttcaggata acgggtacac
     6961 caaagaagag atgaagatgg tgtgggagac ccagaagatc atggaagaca aaaacatacg
     7021 ggtaaacgcc accgcggtgc gcgtaccggt gttttacggg cactccgagg ccgttcatat
     7081 cgagacttca cgcaagatca gcgccgatga ggcaagggcg ctgctgcaga aggcgcccgg
     7141 ggtgaccgtt ctggacgaac gtcagcccgg cggctatccg acagcggtta ccgaagcagc
     7201 gaaccacgac acggtttatg taggacggat tcgcgaagat atgtcgcatg agcggggtct
     7261 cgacctttgg attgtcgcgg acaacatccg caaaggcgct gcgaccaaca gcattcaaat
     7321 cgctgagatt ttggttcgcg agtatttata agttatattg cgacccaaat aggcgcaggg
     7381 cctaggtttg cgtcttaatt acatgtttta taaggattac tcagccgcct tgtgcgctgg
     7441 gagataagat gttcaagcga aacttatgcc aacttctgtt gctggcattg ctatccccgg
     7501 ccgcgacgtt cgcgctgggc ctcggagaga ttcacctcaa gtctgccctg aacgcaccgc
     7561 tggacgccga aatcgatgtc gtcggagcca gccccgagga tcttgccgga ctgaaagccc
     7621 gactggcccc gcgcgacgcg ttcgtgcgtg agggcctgga ctatccctct tatctgaaca
     7681 cgctcagcgt gagtgctgag aagacctccg acgggcgcaa tgtgctgcat ctgcataccg
     7741 gtgacccggt cagtgagccg ttcgcgacgc tactggttga agcggactgg ccgcgcggcc
     7801 atgtggtgcg cgagtacacg gtactcattg atccgccggt ttttagcgct cagaacgcga
     7861 ccaatgccgc ggtcgcggcg cccgcaaccg gcgctacggt ccgcagcggt gccgttgagc
     7921 gacccgcgga gcgtgccagc gcaggctcga catcatcagc aaacacttcc gcgccgtcgg
     7981 ccgccggttc taccagcggc gggcgcgctg gcgcagctgc tgccgg
//
LOCUS       DATMRM010000484         4174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2138365, whole genome shotgun sequence.
ACCESSION   DATMRM010000484 DATMRM010000000
VERSION     DATMRM010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2138365"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VNZ06_14710"
     CDS             complement(<1..923)
                     /locus_tag="VNZ06_14710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW22048.1"
                     /translation="MSAPAVELARPNLAAKRLRVGLIGEYPVAEDRIQEGGVQSVTYA
                     LAHALARRPDIECHIVSAMSDTTTDYRQVGALHVHYVRRMRLPRLVSLRLHDVPALRS
                     VIRCLKPDVVHGQAQDRHALAALNSGLPTVITPHGVLFIESRMLKRSPTDVVGGFKQY
                     LVNSMEREVFARARDMIIISRYLPQVYGPMLRARTHFIDNPIGQEFFQIERAPEAGRL
                     LFVGTVVPRKRVPDLVKALGRVLQICERAGRSPAWREPLQLRIAGPLLDPGTEAQVRS
                     AITSAGLERRVVFLGPISQSQLLDEYARAQL"
     gene            complement(925..1866)
                     /locus_tag="VNZ06_14715"
     CDS             complement(925..1866)
                     /locus_tag="VNZ06_14715"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWW22049.1"
                     /translation="MSKYLVTGGCGFIGSAVVNCLLERGEQVVIVDNMSVGKDRWVDA
                     SRKPTLLKEDIRNPEACARILRDTKPETVLHLAAHHFIPLCEDNAYAAYELNVYGTLN
                     VLEQARVNGVRNFFLASSGDVYPPNFVPHREVDAVSPVYIYGHTKMMAEQMCMRYFES
                     KTCFRTLLIGRLFNAAGPRETNPHLLAEVTKQVARDGKKLIEVGNLWPLRDYVDVDSM
                     ASAILDATAKVEGLEILNFGSGKAVEVKEALRILTSVLPFGIQIKSVPERQRPNDRPF
                     LCPDTTRLRRAIGRSADSFNEQTARKIFAEYPGLLTG"
     gene            2012..3223
                     /locus_tag="VNZ06_14720"
     CDS             2012..3223
                     /locus_tag="VNZ06_14720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWW22050.1"
                     /translation="MTTRIAIFHPADPAGHVPGGIDTIIRGMLKWAPSDLDYTIFGAT
                     SDESSRPVGREARLEFGGPNVHFVPLTRIDPSSRRAAIPVTLRYMLAVRTQMKRGLFD
                     SFDALDFHRIETLWLFRHDRRPKNIIVHQDMSIIRDPGCDIAWRHAPWLYERIEGGVL
                     PSAAHVYAVRQTAVERYRKTYPQLAQRISFLPTWVDTAIFTPPADAAERDTARATLRA
                     RLGLSDASVRVLTTVGRLDKQKDPLLLIEAFAQASPQANLHLVLVGDGVLRSNVEQLC
                     STLKLSGQVSFVGVQPPLQIASILRGSDLFVLSSAYEGMPIAVLEALATGLPVVSTNV
                     GEIPLLIKNGISGQIASERTAQGLSAAVGAALDQVAAMRGKPSEDAVSPFRPELILRS
                     IYENHRSQQAG"
     gene            3463..>4174
                     /locus_tag="VNZ06_14725"
     CDS             3463..>4174
                     /locus_tag="VNZ06_14725"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWW22051.1"
                     /translation="MTENGYEVTATGRAVTVLEIARRDQLAQVGVRVVEGSLLDVEIS
                     KQVVSGCDAVIHLAAAQHEGNVPDSYFHDANVVGTRTLIDAAVNAGVRRFVYGSTIGV
                     YGHASLGELDESSPTSPDNIYTRTKLEAEQTVTEYSQRIEVCIARISETYGPGDMRLL
                     KLFRGIGRGRFILLGDGENVRQVIYVEDLCRALLLASELPAAVNQTFVFSGSDVMTTN
                     TMVKTIGQAFGRRGSTLRI"
BASE COUNT          848 a         1312 c         1224 g          790 t
ORIGIN      
        1 aacagctggg cgcgggcata ttcgtccaac aattggctct ggctgatcgg acccaggaac
       61 accacccggc gctcaagtcc ggctgaagtg atggcactgc gaacctgagc ttcggtaccc
      121 ggatccagaa gcggcccggc aatccgcaac tgcagtggct cccgccaggc gggggatcgc
      181 cccgcgcgct cacaaatctg caacacgcgg cccaaggcct tcaccagatc tggcacccgc
      241 ttgcgaggca cgaccgtacc gacaaacagc aggcgtccag cctcaggcgc acgctcgatc
      301 tgaaagaact cctgcccgat gggattgtct atgaaatggg tacgcgccct cagcatagga
      361 ccgtaaacct gcggcaggta gcgcgagata atgatcatgt cgcgcgcgcg cgcaaaaacc
      421 tcacgctcca tgctgttgac gagatactgt ttgaagccgc cgacaacatc ggtcgggcta
      481 cgcttcaaca tccggctctc gatgaagagt acgccgtgcg gagtgatcac tgtcggcagg
      541 ccactgttca gagcggccaa tgcgtgccga tcctgcgcct gaccgtgtac cacgtcaggc
      601 ttgaggcagc gtatgaccga gcgcagcgcg ggcacatcat gcaagcgcag gctgaccaga
      661 cgcggcagac gcatccggcg gacgtagtgc acatgcaacg cgcccacctg tcgatagtcc
      721 gtggtggtat cgctcattgc ggaaacgatg tgacactcaa tatccggacg acgggccaga
      781 gcatgcgcca gggcatacgt cacagactgg accccaccct cctgaatacg gtcctccgcg
      841 accggatact caccgatcaa tccgacccgc aagcgtttag ccgcgagatt gggccgggca
      901 agctctaccg ctggcgcact cacatcagcc ggtcagaagg ccgggatact ccgcgaagat
      961 cttgcgcgcg gtctgctcat tgaagctgtc agcgctacgc ccgatggcgc gacgcagccg
     1021 cgtggtatcg gggcacaaga atggacggtc gttgggtcgc tgtcgctccg gcactgactt
     1081 gatctgtatc ccgaacggca gcaccgaggt caggattcgc agcgcctcct tgacctcgac
     1141 cgctttgcca gagccgaaat tcaggatctc cagtccttcc actttggcgg tcgcatcgag
     1201 gatggcggaa gccatggaat ccacatccac atagtcgcgc agcggccaca gattgccgac
     1261 ctcgatgagc ttcttgccat cgcgcgcgac ctgtttggtg acttccgcca gcagatgcgg
     1321 attggtctca cgtgggccgg ctgcattgaa caggcggcca atcaacaacg ttcgaaaaca
     1381 tgtcttcgac tcgaaatagc gcatgcacat ctgctctgcc atcatcttgg tatgaccata
     1441 gatgtagacc ggactgacgg catcaacctc ccggtgcggc acgaagttgg gcggatagac
     1501 atcaccggaa gacgccagga agaagttacg caccccattc acacgcgcct gctccaacac
     1561 gttgagcgtg ccgtagacat tcagctcgta ggcggcgtag gcattgtcct cgcacagcgg
     1621 aatgaagtga tgagccgcca ggtgcagcac ggtttccggt ttggtgtcac gcagaatgcg
     1681 cgcacacgct tcgggattgc ggatgtcctc cttcaacaac gtcggtttgc gagacgcgtc
     1741 cacccagcgg tctttgccga cgctcatatt gtcgacaatg acaacctgtt cacctcgttc
     1801 cagcaggcag ttcacaaccg ccgaaccgat gaatccgcag cccccggtca ccaggtactt
     1861 gctcattgat caatacactc ctgactcgcg cgtcgcgcgc gagcaagata ccaatttctg
     1921 gcatttgacg gtacgttttc agcggcgccc cccaccggaa ctgtgatcgg gaccacaccg
     1981 agcgaaaaac ccgatacggc agcatcagaa gatgactaca cgcatagcga tatttcatcc
     2041 ggcagatccg gccggtcacg tacctggcgg aatcgatacc atcatccgcg gcatgctgaa
     2101 atgggcgcca tctgatctgg actacacgat tttcggcgcg accagtgacg agtcatcccg
     2161 gccggtgggc cgcgaagccc ggcttgaatt cggcggaccg aatgtgcatt ttgtgccgct
     2221 cacgaggatc gatcccagct cgcgacgggc cgcgattcca gtcacgctgc ggtacatgct
     2281 ggcagtgcgc acgcagatga agcgtgggct gttcgacagt ttcgatgcgc tcgattttca
     2341 tcgcatcgag acgctgtggc tgtttcgtca cgaccgtcgc ccaaagaaca tcatagtgca
     2401 ccaggacatg tctatcatcc gcgaccctgg ctgtgatatc gcttggcggc acgcaccgtg
     2461 gctgtacgag cgcatcgaag gcggcgtgtt gccgagcgcg gctcacgtct acgccgtgcg
     2521 tcagaccgcg gtcgagcgct accgcaaaac ctatcctcag ctggcgcagc gaatctcctt
     2581 tcttcctacc tgggtggaca ccgccatatt cacaccaccc gcggacgctg ccgaacgcga
     2641 cacggcgcgc gccacactgc gcgcgcgtct cggactgagt gatgcgagcg tgcgcgtatt
     2701 gaccactgtc ggccggctgg acaagcagaa ggatcccctg ctgctgatcg aggcgttcgc
     2761 gcaggcatcg ccgcaggcca atcttcacct ggtgctggtt ggagacggcg tattgcgatc
     2821 caatgtggaa caactgtgca gcacgctgaa gctgagcgga caagtcagtt tcgtcggcgt
     2881 gcagccaccg ttgcagatcg cgagcatcct gcggggcagc gacctgttcg ttctgtcctc
     2941 ggcctatgag ggcatgccca tcgcggtgct ggaagctctg gcaaccggtc tgcctgtggt
     3001 cagcaccaat gtcggcgaga tcccgctctt gatcaagaac gggatcagcg gccaaatcgc
     3061 ctcggagcgc actgcgcagg ggctgtcggc agcggtcggc gcggcgctcg atcaagttgc
     3121 cgcgatgcgc ggcaaaccca gcgaagacgc agtatcgccg tttcgccccg aactgatcct
     3181 acgctcgatc tatgagaacc accgctcgca gcaagctggc tagtgcattc ggcgcgcaag
     3241 ttcaccctga ttcagggact tgcacggtcg cgagtgtgga tggtgtccgt tgccctgctc
     3301 accttttctg cctatcatgt ctcacggcag ttcgagcttt gcagattcct gcgccccctg
     3361 ctggaactgt gacaagcctc tcactaagag cgacgacaag gaatcatgcg aatcgctgtc
     3421 actggtacga ccgggtttat cggctgttac ctggcacaat acttgaccga aaacggctat
     3481 gaggtcacag cgacgggccg tgccgtcacc gtactggaga tcgcgcgccg ggatcagctc
     3541 gcccaagtgg gcgttcgcgt ggtcgaaggc tcgctgctcg atgtcgagat ctcgaagcaa
     3601 gtggtcagcg gctgcgatgc cgtgatccat ttggcggccg cccagcacga aggcaacgtt
     3661 ccagattcct actttcacga cgccaatgtc gtcggtaccc gcacgttgat cgacgcggcg
     3721 gtaaatgccg gcgtccgacg cttcgtctat ggcagcacca ttggcgtcta tggccacgcg
     3781 agcctcgggg agctggatga gagcagcccc acttccccag acaacatcta cacccgcacc
     3841 aaactcgaag ccgaacagac ggtgaccgaa tactcgcagc gtatcgaggt ctgcattgcg
     3901 cgtatttccg agacctacgg ccccggcgat atgcgactgc tcaagctgtt caggggcatc
     3961 ggccgcggca gattcattct cttgggcgac ggtgagaatg tgcggcaggt gatctacgtt
     4021 gaggatctat gccgggcgct gctgctggcc agcgagctgc cggcggcggt caaccagacc
     4081 tttgtgttct ccggctccga tgtgatgacc accaacacga tggtcaaaac gattggccag
     4141 gccttcggcc ggcgtggctc cacactgcgc atcc
//
LOCUS       DATMRM010000485         6093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3993725, whole genome shotgun sequence.
ACCESSION   DATMRM010000485 DATMRM010000000
VERSION     DATMRM010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3993725"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VNZ06_14730"
     CDS             <1..212
                     /locus_tag="VNZ06_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HWW22052.1"
                     /translation="LLLHRKEIDRLIGAVERRGYTVVPLELYWKHGRAKLQIGLAKGK
                     KQHDKRATEKERDWQRDKARLMRRG"
     gene            270..2417
                     /locus_tag="VNZ06_14735"
     CDS             270..2417
                     /locus_tag="VNZ06_14735"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWW22053.1"
                     /translation="MSSKPHPATYDLSVSGMTCAGCAGRVERTLRAVDGVLQASVNLA
                     TEKAHVQAIGDLRPSDLIAAIERAGYRAQSTYEAVRTQGSPGETGRVIAALALSMPLI
                     LSMLGIPVAAWLQCAAATPIQFVLGARFYVGAFKAVRDGTGNMDLLVALGTSTAYFYS
                     LYGTLHPTPGWHLYFEAGSFVIALVLLGKWLESRAKHSATAAIRSLMALRPERARIER
                     DGGEIEVVMDEVRPGDVVIVRPGEQVPADGKVLSGVSAVDESLLTGESLPVEKSRGSG
                     VTGGAINGTGLLRVTTTAIGAQSRLARIIALVETAQMQKAPVQRLVDRVASIFVPIVL
                     LIALLTFLGWWWFTASIAAGIIPAVSVLVIACPCALGLATPCALLVGTGTAARRGILI
                     RDIQALEQARAIDTVLLDKTGTVTQGRPAVTEIATHGIDESELLRLTAAAQMGSEHPL
                     ARAVLARTPMRSLPELSDFRSQPGRGLTARIGGRRIAVGNRALMRASQLDLSPLESRA
                     RELEDQARTLMWVASLEPHVELLGFIAVADPLRPTAIQAIDRLRRLGIEVALLTGDNA
                     RTAQAVAAELGLPRVMAEMTPEGKAAQVRREQAAGRHVAMVGDGVNDAPALAAANVGI
                     AMGGGTDVAMQSAGITLMRPDPTLIASAIEISRATYRKIRQGLFWAFAYNVVGIPLAA
                     AGLLNPMIAGAMMALSSVSVVTNALSLRRYAHQ"
     gene            2430..2846
                     /locus_tag="VNZ06_14740"
     CDS             2430..2846
                     /locus_tag="VNZ06_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HWW22054.1"
                     /translation="MSADAELSIGEAAARSGVPARTIRFYEQAGIIKRAARSLNRYRR
                     YSEAEVQTLRFVQRARALGFPLKDIADLLGLYRNTRRASRDVKKLALGHIAQLDRKIA
                     DLASIRSTIATLAERCHGNDRPACPILDELGAPSHH"
     gene            2855..3052
                     /locus_tag="VNZ06_14745"
     CDS             2855..3052
                     /locus_tag="VNZ06_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22055.1"
                     /translation="MPIGETIEAMMRFELSVIAAGHCTGWRAMSALSNTFGATLDPMC
                     VGKRYRFGGGAQQSTGGVRGP"
     gene            complement(3060..3932)
                     /locus_tag="VNZ06_14750"
     CDS             complement(3060..3932)
                     /locus_tag="VNZ06_14750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWW22056.1"
                     /translation="MQPTQAAAPETRNEGQKSSLNGHGLHRAGSSGLAETVVREEGFG
                     ASDRHRLVAQINELSMANRRVDEFLAILSHELRSPLASIQHGIGVLRGPSGDDVIVQR
                     GMHELIEWQVNQMRGIAAGLLDVARLTSGRLPLRPERLELRALLSNAIETLEPDFNRR
                     TQALVASWPSSSLWVKSDADRVEQVFTTLLTNASKYTDVGGSVALSLSAQDKYAIVRI
                     KDSGIGIAAHALPHIFDVFMQADAASARCRSGMGTGLALARRIVESHGGSVEAHSAGL
                     GQGSEFIVRLPLEN"
     gene            complement(4187..5053)
                     /locus_tag="VNZ06_14755"
     CDS             complement(4187..5053)
                     /locus_tag="VNZ06_14755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWW22057.1"
                     /translation="MPPDCETSYRSGQSPAAPAAGNGLDGQPSIHGDEPLEGSATSQF
                     DRRLHNARTEVLGGADRRKDEFLATVCHELRGPIGAIRNAVKVLRCQNDFKVAVQHHM
                     HELIERQAQQMAFLVSGLLDITRIVRGQLALQRERVDLRAVLGNAVETLEWDLKGRSH
                     QLSLTLPESSVWLVADGARLEQVFVNLLGNASKYTQIGGELALSLHVSGGSAIVRVRD
                     SGIGISPDALPHIFDLFMQAEAAVPRSGSGLGIGLALVRAILESHGGNVTALSAGLGQ
                     GSEFIVCLPLEP"
     gene            5325..5822
                     /locus_tag="VNZ06_14760"
     CDS             5325..5822
                     /locus_tag="VNZ06_14760"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWW22058.1"
                     /translation="MNAQSGIDPTVFSPKPTPCANPADLVEAFTAAFTSDDVESFYRL
                     ISAQAEWVIMATGETFRGLDQIKQLTRRPMAARTHRGGPGIKPTTVFSSADGSMLCWE
                     YVHTGIVIDKWPASSLQRPAPGTTFELPIILVAEIREGKIIKMREYFDLLTLTESGAP
                     HHLYS"
BASE COUNT         1155 a         1909 c         1892 g         1137 t
ORIGIN      
        1 aactgttact ccatcgcaag gagatcgacc ggctcatcgg cgcggtcgag cggcgcggct
       61 acaccgtcgt gccgctcgag ctgtactgga agcacggacg cgccaaactg cagataggcc
      121 ttgccaaggg caagaagcaa cacgacaaac gtgctaccga aaaggagcgc gactggcagc
      181 gcgacaaggc gcgtctgatg cgacgcggct gagcttgacc ttccagcgac tggaagctgc
      241 acaatccagt caactggaag gttgagtgca tgtctagcaa gccacacccc gccacatacg
      301 atctgtccgt cagcggaatg acctgcgcag gctgcgcggg tcgagtcgag cgaacattgc
      361 gagcggtcga cggcgtcttg caggccagcg tcaatctggc gaccgaaaaa gcccacgtgc
      421 aggcgatcgg cgatctgcgg ccttcggacc tgattgccgc gatcgagcgc gcgggttatc
      481 gcgctcaatc gacctacgag gctgttcgga cgcagggatc gccaggcgag acgggtcgcg
      541 tcattgcggc gctcgcactg agcatgccgc tgattctctc gatgctgggg atcccggtcg
      601 cggcctggtt gcaatgcgct gcggcgacac cgattcagtt cgtgctgggc gcacggttct
      661 acgtgggcgc cttcaaagcc gtgcgcgacg gcacgggcaa catggacctg ctggtcgcgc
      721 tcggcacctc cacggcctac ttctacagcc tctacgggac gctgcacccg actccggggt
      781 ggcatctgta tttcgaggcg ggttcattcg tcatcgcctt ggtgctgctc ggtaagtggc
      841 tggaatcgcg cgccaaacac tccgccacgg cggccatccg ctccctcatg gcgctgcggc
      901 ccgagcgggc acggatcgag cgtgacggcg gtgagatcga ggtcgtcatg gacgaggttc
      961 gtcctggaga tgtggtcatc gtgcgtccgg gcgagcaggt gccggcggac ggcaaggttc
     1021 tgtcaggggt cagtgctgtc gatgagagcc tgctgaccgg agagagcctg ccggtcgaga
     1081 aatcccgcgg ctcaggcgtc accggcggtg cgatcaacgg cacgggtttg ctgcgcgtga
     1141 caaccaccgc gatcggcgcc cagtccaggt tggcacgcat catcgcgctg gtcgaaaccg
     1201 cacagatgca gaaagcaccg gtccagcgcc tggtcgatcg ggttgcctcg atcttcgtgc
     1261 cgatcgtgtt actgatcgcg ctgctgacct tcttgggctg gtggtggttc accgccagca
     1321 tcgcggcggg aatcattccg gccgtctccg tgctcgtgat cgcttgtccg tgtgcgctcg
     1381 gattggcaac accctgcgca ctgctggtcg gcaccggcac tgccgcgcgc cgtggcattt
     1441 tgattcgcga tattcaggcg ctcgaacaag cgcgcgccat tgacacggtg ctgctcgaca
     1501 aaaccggtac cgtcacgcaa ggccgtccag ccgtgacaga gattgcgacc catggaatcg
     1561 acgaatccga gctgctgcgg ctcacggctg cggcacagat gggcagcgaa catccgctcg
     1621 cgcgcgccgt actggcgcga accccgatgc gctcactgcc agagctcagc gatttccgca
     1681 gccagccggg ccgtggactg acggcgcgca tcggcggccg gcggattgcc gtgggcaacc
     1741 gcgcgttgat gcgcgcctcg caactcgacc tgagcccgct ggaatcccgc gctcgcgaac
     1801 tggaggatca agctcgaacc ctcatgtggg tcgcctcact cgaaccgcac gtcgagctgc
     1861 tcggattcat tgcggtcgcc gatccgctca ggcccaccgc gattcaggcg atcgaccggc
     1921 tgcgccggct cggcatcgaa gtggcgctgc tgacgggcga caacgcgcgt accgcccaag
     1981 ccgtcgcggc cgagcttggg ctgccgcggg taatggccga gatgacaccc gaaggcaaag
     2041 ccgcgcaggt gcgtcgggag caggccgcgg gcaggcatgt cgcgatggtc ggcgacggcg
     2101 tgaacgacgc cccggcgctg gcggccgcga acgtgggcat cgccatgggc ggtggtacgg
     2161 acgtcgcgat gcagtcggcg ggtatcacct tgatgcgccc ggatcccacc ttgatcgcga
     2221 gcgcgatcga gatcagccgc gcgacctatc gcaagatccg acaggggttg ttctgggcat
     2281 tcgcgtacaa cgtggtcgga ataccattgg cggctgcggg gctgctcaat ccgatgatcg
     2341 ccggtgcgat gatggcgctg tcgtcggtca gcgtcgtgac caatgcgcta tcgttgcgtc
     2401 gctacgcgca ccagtaaccg gagaacccaa tgagtgcgga cgcagagctg agcattggag
     2461 aggcggctgc ccgctcgggt gtgcccgccc ggaccattcg attctacgaa caggccggca
     2521 tcatcaaacg cgccgcacgc agcctgaacc gctaccggcg ctactcggaa gccgaggttc
     2581 agacgctgcg tttcgtgcaa cgcgcacgcg cgctcggttt cccgctcaag gatatcgcgg
     2641 atctgttagg gctgtatcgc aacactcgtc gcgccagccg cgacgtcaag aagcttgcat
     2701 tggggcacat cgcccagctc gaccggaaga tcgcggacct ggcttcgatc cgcagcacca
     2761 tcgcaacgtt ggcggagcgc tgtcacggca acgaccgccc tgcctgtccg atcctggatg
     2821 agctcggcgc gccgagccat cactgagcga gcaattgccc attggcgaga ccatcgaggc
     2881 gatgatgcgt ttcgaactgt cagtgatcgc tgcggggcat tgcaccggct ggcgcgcgat
     2941 gtccgcgctc agcaatacct ttggcgcgac gctcgatccg atgtgcgttg gcaagcgcta
     3001 tcgattcggc ggcggcgcgc agcaatccac cggcggtgta agggggccct gagtcaggat
     3061 caattctcga gcggcagacg cacgatgaat tcgctacctt gtcccagacc ggcactgtgc
     3121 gcctccacgc tgccaccgtg cgattctacg atcctgcgtg ccagcgccag accggtgccc
     3181 atcccggacc tgcagcgcgc cgatgcggca tcagcctgca tgaaaacatc gaagatatgc
     3241 ggtagagcat gcgcggcaat accgatgccg gagtctttga tgcgcacgat cgcgtactta
     3301 tcctgcgcac tgagcgacaa cgccacgctt ccgccgacgt cggtgtattt ggaagcattg
     3361 gtgagtaggg tcgtgaagac ctgctcgacg cggtcggcgt cactcttgac ccataggctc
     3421 gaggaaggcc acgacgccac cagtgcttgc gttcggcggt tgaagtccgg ctcaagtgtt
     3481 tcgatcgcat tgctcaacag ggctcgcaat tcgaggcgct ctggccgcag tggaagacgg
     3541 ccgctggtga ggcgtgcgac gtcgagaagt cccgcggcaa tccccctcat ctgattcacc
     3601 tgccattcaa tgagttcgtg cattccgcgc tgcacaatta cgtcatcgcc acttgggccg
     3661 cgcaatacgc cgatgccgtg ctggatcgac gcaagcgggc tccgcagttc gtgggagaga
     3721 atcgccaaaa actcgtctac ccggcggtta gccattgaca gctcattgat ttgcgcaacc
     3781 agtcggtgtc gatcactagc cccgaagccc tcttcacgga cgaccgtctc tgccaggccg
     3841 ctggaaccgg ctcggtgcaa tccgtgacca ttgagggagg atttctggcc ttcgttccga
     3901 gtctctggcg cggcggcttg agttggctgc atatcaccct ctgctctcag tgccagctga
     3961 agattaaggt gcggattgca gaatcatctg tacgccacca gaccaactga gctgaatgga
     4021 aatgccaggt cgtgcgattt cctcgacgat gctagacgct gccacggata gaagtacgtc
     4081 gaaggtcggc gcacgtacgc cgctcgagct gcgcccggtt agtcaccgtg atatggccgc
     4141 gcagtcccgc tccagctcac accagccgat acccgatcgg atgagatcac ggttcaagcg
     4201 gcaggcacac aataaattcg cttccctggc cgagaccggc actgagcgct gtcacgttgc
     4261 cgccatgcga ttccaaaatt gcgcgcacca gggcgagacc gatgccaaga ccggagccgg
     4321 agcgcggcac tgctgcttcg gcctgcatga agagatcaaa gatgtgcggc agcgcatccg
     4381 ggctgatgcc gattccagaa tcccgaaccc gaacaatcgc gctccccccc gacacatgca
     4441 gcgacagggc gagctcgccg cctatttgcg tgtacttcga tgcattgcca agcaaattca
     4501 cgaacacctg ctccaatctc gcaccgtcag ccaccagcca cacgctggat tcgggcaagg
     4561 tgagcgaaag ttggtggctg cgtcccttaa gatcccactc gagcgtctcc accgcattgc
     4621 ccaaaaccgc acgcaaatcc actcgctcgc gctgcagtgc tagctgccca cggacgatgc
     4681 gcgtgatgtc gagcaggccg ctgaccagga aggccatctg ctgggcctgc cgctcgatga
     4741 gttcgtgcat atgatgctgc actgcgactt tgaagtcgtt ctggcagcgt aacaccttga
     4801 cggcattgcg aatcgccccg atcggaccgc gcagctcgtg gcacaccgtt gcgaggaatt
     4861 cgtctttgcg acgatcggca ccgccaagca cctcagttcg cgcgttatgc agtcgtcgat
     4921 cgaattggct ggtggcgctc ccctccaaag gctcatcgcc atggatactc ggctgcccgt
     4981 ctaaaccgtt gcctgccgct ggcgcggcag ggctctggcc ggacctgtat gacgtttcgc
     5041 aatcaggcgg catcggttgt ctccacccct tgtttgctgt cagcgaaaga ctacgggcgg
     5101 cgctgttcct gactctgtgc ggtatcagac ataaccgcga tatcagttgt ggcaggcggc
     5161 gaacgatcag agcgctgagg gcgatattgc tagatcagtt gcactcatct gcgtgcgcta
     5221 gcgcccagac gagtgcaagg cacaagcaca atctgcggct atcagccggg tgcggtttgt
     5281 tagcgcatgc agcagatttc aaccgggcac aggtggagaa cgcaatgaat gcccaaagtg
     5341 gaatcgatcc gacagtgttt tccccgaagc ccacgccgtg tgccaacccc gccgacttgg
     5401 ttgaggcgtt cacggcagcg tttacctctg atgatgtcga aagtttctac cggctcattt
     5461 cggcgcaggc cgagtgggtg atcatggcga ccggtgagac attccggggg ctcgatcaaa
     5521 tcaagcagct gacgcggcga cccatggcgg cgcgaacgca tcgcggtgga ccaggtataa
     5581 aaccgacgac tgtgttcagc agcgctgacg ggtcaatgct ttgttgggaa tacgtgcata
     5641 ccggcatagt catcgacaag tggcccgcgt cgtcacttca gaggcccgct ccgggtacca
     5701 ctttcgagct gccaatcatc ctggtggccg agatccgtga gggcaagatc atcaagatga
     5761 gggagtactt tgacttgctg acgctgaccg aatctggcgc cccacatcac ctctattcct
     5821 aggccaagcg atccgcgctt tgggcgcacc cggcggcagg cgagcgagac ttacctgtgt
     5881 gtgctagcga gcagacgatt caatagccgc ccctttattc ttatttcagt agtacgcggc
     5941 gacgcttgtt ggcgacgtca acattgggga ctcgacacgg cctgtctacg acactagctc
     6001 taatcggcac tagctctaat cggcactagc tctaatcggc actagctcta atcggcacta
     6061 gctctaatcg gcactagctc taatcggcac tag
//
LOCUS       DATMRM010000486         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3995274, whole genome shotgun sequence.
ACCESSION   DATMRM010000486 DATMRM010000000
VERSION     DATMRM010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3995274"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VNZ06_14765"
     CDS             <1..434
                     /locus_tag="VNZ06_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22059.1"
                     /translation="SASVQVNPDSGMDHYFAADAVAPSHHDRVGVSYYRTERVPNENV
                     PGTIFAPGMPGVQQSNSDYVLAGGRGLNAPFSFVVVSPEFPAPDGVQTGFNGDYSGLC
                     INKENEAHPLWSDTRNVNPFPLNGSTHDEDIFTMSVELPGR"
     gene            777..>2031
                     /locus_tag="VNZ06_14770"
     CDS             777..>2031
                     /locus_tag="VNZ06_14770"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HWW22060.1"
                     /translation="MAAWTLRWPPAAAEMIEITVVIFIVVYLGMILGGLPVVQIDRTG
                     IALLGAIALIGFDAVSPDEAARSIHLPTLILLFAFMVVAAQMRLSGFYDAVARRLTRL
                     PLTPPLLIGALIFVVAVLSAVFSNDIVCLAVAPVLIAACRGLRLDPRPFLLALACGAN
                     IGSAATLIGNPQNMLIGQTLHLSFGGYFLEAVLPVGVSLLATWILMLALTRGRFVDAA
                     AVAPRVGTRDSEAPDEGVPLDTWQTIKGLTVALTIVACFVFGPWPREVVALAGAGVLL
                     TSRRLHSRQMLGLVDWELLVLFIGLFVVNHALQKTGVPARLVADLTATGVSLQHLGPL
                     FATTFVLSNIVSNVPAVMLLLPAVEHTLAGPTLALVSTLAGNLFIVGSVANIIVVDVA
                     ARHGIKIGWREHARIGIPVTLATLAI"
BASE COUNT          327 a          627 c          670 g          407 t
ORIGIN      
        1 ggagtgcaag tgtgcaggtt aaccccgact cgggaatgga ccactatttc gcagcggacg
       61 cggtcgctcc ctcgcaccat gaccgggtgg gcgtcagcta ttaccgcacc gagcgggttc
      121 cgaacgaaaa cgtgcccggc actatcttcg cccctggaat gcccggcgtg cagcaaagca
      181 actcggacta tgtcctggcg ggaggtaggg gcctgaacgc gccgttcagt ttcgtggtcg
      241 tttcgcccga attcccagcc ccggacggcg ttcagaccgg gttcaatggc gactacagtg
      301 gactctgcat caacaaagag aacgaggcgc atccgctctg gtcggatacg cgcaacgtga
      361 accccttccc gctgaacggc tcgacccatg acgaggacat cttcaccatg agcgttgaac
      421 taccggggag gtaattgggc accgtcttcg agcgcaaaac gcgatcgaag gtcgccgagt
      481 cgacaaataa gagaactcgg ccttgtgccg ggttttcttt tttgtggtgc acccccacca
      541 gcagggcgca gccacttgga ggtggaagtc ctctatagac ggcggtggat gattggatgg
      601 tggccaaggg ctccgtcaag accggcatgg atgctggatt cgaagcgagt ggggcaagga
      661 ttcaccggct gatcaccgcg caagctgatg gcacttcggt gatcttgaat cggccgcaac
      721 tggccggacc ccgaaatcgc cgagtctggt cggatgcggt catttaaaac tgcgtcgtgg
      781 cagcatggac gcttcgttgg ccgccggccg ccgcagagat gatcgaaatc acggtcgtaa
      841 tattcatcgt cgtctacctg gggatgatcc tcggtggcct gccggtcgtg cagatcgacc
      901 gcaccggcat cgcgctgctc ggcgcgatcg cgctgatcgg cttcgatgcg gtgagccctg
      961 acgaagccgc gcggtcgatc cacttgccga cgctgatcct gctgttcgcc ttcatggtcg
     1021 tggcggcgca aatgcggctc agtggcttct acgacgcggt ggcgcgccgg ctgacgcgac
     1081 tgccgctgac gccgccgctt ctgatcggcg cactgatttt cgtggtcgcc gtgctgtcgg
     1141 ccgtgttcag caacgacatc gtctgcctcg ccgtggcgcc tgttctgatc gccgcttgcc
     1201 gcggccttcg cctggacccg cggccgttcc tgcttgcgct ggcctgcggc gcgaacatcg
     1261 gttcggctgc gacgttgatt ggcaacccgc agaacatgtt gatcggccag actctgcatc
     1321 tgtcgttcgg cgggtacttt ctcgaggcgg tgctgccggt cggggtgagt ctgctggcga
     1381 cctggatact gatgctggcc ttgacgcgcg gccggttcgt ggacgcggcg gccgtcgcgc
     1441 cgcgcgtcgg aacgcgcgac agcgaggcgc ccgacgaggg agtcccgctg gacacctggc
     1501 agacgatcaa aggtctgacc gtggcgctga cgatcgtcgc gtgcttcgtg tttgggccct
     1561 ggccgcgcga ggtggtcgcc ctggcgggag ccggcgtgct gctgacgagc cgccgcctgc
     1621 actcgcgcca gatgttgggc ctggtcgact gggagttgct tgtgctcttc atcggcctgt
     1681 tcgtggtcaa ccacgcgctg cagaaaaccg gcgtcccggc acggctggtt gccgatttga
     1741 ctgccactgg ggtgtccctg cagcacctcg gcccgctgtt cgcaacgacc ttcgtactgt
     1801 cgaatatcgt ttccaacgtg cccgccgtaa tgctgctgct gcccgccgtg gaacacacgc
     1861 tggctggccc cacgctggcc ctggtgagca cgctggcggg caatttgttc atcgtcggca
     1921 gtgttgcgaa cattatcgtc gtcgacgtgg cggcgcggca tggcatcaag ataggatggc
     1981 gcgagcatgc gcggataggc atcccggtca cactcgcaac gctggcgatc g
//
LOCUS       DATMRM010000487         3716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_256908, whole genome shotgun sequence.
ACCESSION   DATMRM010000487 DATMRM010000000
VERSION     DATMRM010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256908"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..409)
                     /locus_tag="VNZ06_14775"
     CDS             complement(116..409)
                     /locus_tag="VNZ06_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22061.1"
                     /translation="MYRFLLKPCNPADLVSTIERALDHKRLEEHSRSLLRQFRKQAAL
                     LNALERERPGLLSSELDDSRGLDSHDVSSGDLAQDLQRELGVSQDAVRRWLEH"
     gene            1022..1471
                     /locus_tag="VNZ06_14780"
     CDS             1022..1471
                     /locus_tag="VNZ06_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22062.1"
                     /translation="MASDTAAALPSASTSISSEEATKGAVKKVTAPVLSLPALPVPDQ
                     PWLSTVHDAALQIAQSPKVSDALTLSHIECQGSKCEISGSTKVSSDGQAHGSSDVARL
                     LKAMNEGQVAGGDTGRWAGVTQIAVTSSAVRRWWPSARSRTAWQGRW"
     gene            1601..1858
                     /locus_tag="VNZ06_14785"
     CDS             1601..1858
                     /locus_tag="VNZ06_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22063.1"
                     /translation="MIEGVRSGTPNYEECSPEFAQVMRTQLPRLHATSVWLGGIVAIE
                     FRGVSSQGADMYDVQREHGRADYRFALGTDGKIYGLAVMMS"
     gene            complement(1874..2239)
                     /gene="speD"
                     /locus_tag="VNZ06_14790"
     CDS             complement(1874..2239)
                     /gene="speD"
                     /locus_tag="VNZ06_14790"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HWW22064.1"
                     /translation="MSGRSVAPAGKHWLVEMYQTPELDDVARIRRALRHAVAESGVRM
                     IRLQLHHFGVGRGVTGVALLAQSHISIHTWPERRYAALDIFMCGSKSNPRKALDALRE
                     ALAPGRVRVRRFRRGLGRV"
     gene            complement(2236..3351)
                     /locus_tag="VNZ06_14795"
     CDS             complement(2236..3351)
                     /locus_tag="VNZ06_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22065.1"
                     /translation="MTIALHAPTVRQPWIHSAAIDGTFILGPALLATAVALLIVLDGQ
                     PQAGLGLWSWALLVIGIDVAHVYSTVYRTYFDPLERAHLSGWLIAVPITTWVAGVLLY
                     SMSAASFWTVLAYTAVFHFVRQQYGFLMLYARAERSLPVWCRRLDQISIYGATLFPLL
                     YWHTHLPRQFVWFIDGDFLPLPDILWSIALPPYVALCLAYLAKEAWLRLRAQPFNIPR
                     NAIVLGTGLSWFFGIVITNGDLVFTLTNVVAHGVPYLALTCIYQRRRDQQLQRPWSLF
                     TLRLLPFAIGLLVLFAFIEEGFWDALVWREHLALFPGFRLLPEIQGIGALSLLVPLLA
                     VPQMTHYVIDGLIWRLRAHPEWRTTLFGRPGAKVHAT"
     gene            complement(3348..>3716)
                     /locus_tag="VNZ06_14800"
     CDS             complement(3348..>3716)
                     /locus_tag="VNZ06_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22066.1"
                     /translation="LAQARRDTVITYYWPLSDQDPATSRTTALKRTLSQWQGFVSADL
                     LRVHPELDGHIENIDVRVWGHAMIRPTPGFIWGDARASATAHQPPIYFAHSDLSGISI
                     FEEAYCRGQSAGTLAARSLA"
BASE COUNT          755 a         1116 c         1210 g          635 t
ORIGIN      
        1 gtgcaattct ccgagcagga tcgcggtcag acgcgggcga aaatcgagaa ctatctggcc
       61 ggcgcgattt cgggcgacaa ggccacgcat acgatgtagg tctaggccgg tctatctagt
      121 gctccaacca ccgccgcacg gcatcctggg acacccccag ctcccgctgc aaatcctgcg
      181 ccagatcgcc gctgctgacg tcgtgggaat cgaggccgcg agagtcgtcc agctcggagc
      241 tgagtagacc cggccgttcc cgctccagcg cgttgagcaa cgcggcctgc ttgcgaaact
      301 gccgcagcag gctgcggctg tgctcttcga gccgtttgtg atcgagcgct cgctcgatgg
      361 tggatacgag gtctgccggg ttgcacggct tgagcaagaa gcgatacaca cctcggcctc
      421 gttgatcgcg cgtaccgccg attcgagatt cccctcgccg gtatggatga tgagaatcac
      481 gttcgggtac ttgcgcctca cggccgccag aaactccgag cccgactctc cgggcatccg
      541 ctcatcggag acaatgacgt ctacgcggtg ccagtcgagc aactggttag cttcatcggc
      601 tgacttcgcg gacagcactt tgaagtgctc tttcgggcaa tgtctgctta ggagagtggt
      661 gatgagcggg tcgcatccac cagcagaatc atcgcggtcg gcctggtcaa cgtcatcttc
      721 gtcccccgcg atcctttcga atcgcttgat tggaacctcg aatgcgctgg tgcagcggtt
      781 cgtatgctac ctccgtctga ggctgaacga gaataggctc gggacagccc ggcacactgc
      841 gtatcgcatc ggtaaggagc gcacgagttg cttcgttgaa cattcgacct ctcttccagg
      901 agggcgcgtg caaacatcta ctgtgattct agtcagcgca gcgaccgcgc taaatctcag
      961 cgtgtggtac ttgtcccggc caacaacaag cacgcggccg actgagtgtg gcaccgaagc
     1021 ggtggcgtcc gacaccgccg ccgccttgcc gagcgcgtcc acttccatca gcagcgagga
     1081 ggcgaccaag ggcgccgtca agaaagtcac tgcgcctgtg ctcagtctgc ccgcactccc
     1141 ggtgcccgac caaccttggc tgagtaccgt ccacgacgcg gcgctgcaga ttgcgcagag
     1201 tccgaaagtc agcgatgcgc tgacgctgtc ccacatcgaa tgtcagggct cgaagtgtga
     1261 gatcagcggc tcgaccaaag tttccagtga cgggcaggcg catggttcct cggatgtcgc
     1321 ccggctcttg aaagccatga atgaaggaca ggtcgccgga ggggataccg gcaggtgggc
     1381 cggagttacc cagattgccg tcacatcgtc ggcagtgagg cgctggtggc cttccgccag
     1441 atcgaggacg gcctggcaag gaagatggtg attacgcagg gcacgatcac gacggaagct
     1501 ccccgcattg acgcggatac cgcacctcgg atcgagcaga cttggcagaa cgcatcagga
     1561 cccgaacccc gcagaccggg agcgagcagg ccctcagcgc ttgatcgaag gggtccggtc
     1621 cggcacaccc aactacgagg agtgcagccc ggagtttgcg caggtcatgc gtactcagtt
     1681 gcctcgtctg cacgcgacat cggtgtggct gggtggaatc gtggcgattg aatttcgggg
     1741 agtcagtagc cagggggcgg atatgtacga cgttcagcgc gagcacggac gcgcagacta
     1801 tcgttttgcg ctgggcacag acggaaagat ctacggtttg gcggtgatga tgtcgtagtt
     1861 caatgggctg ccgtcagaca cggcccaatc cgcgtcggaa ccgccgcacc cgtacgcgac
     1921 cgggcgccag tgcttcgcgc agcgcgtcga gtgctttccg agggttgctc ttgctgccgc
     1981 acatgaagat atcgagtgcg gcgtagcgtc gttccggcca ggtgtggatc gagatgtggg
     2041 actgcgccag cagcgccacg ccggtgacgc cgcggcctac gccgaagtga tgcaactgca
     2101 gccggatcat acgcacgccg gactcggcga cggcgtgtcg cagcgctcgg cggatgcgcg
     2161 cgacatcatc cagctcgggt gtctggtaca tctcaaccaa ccaatgtttg ccggccggtg
     2221 cgacactgcg accgctcatg tcgcgtgcac cttggcgcct ggtcgcccga acagcgtggt
     2281 gcgccattca ggatgcgcac gcagtcgcca gatcaggccg tcaatcacat agtgcgtcat
     2341 ctgtggtacc gccagcagcg gtaccagcag actcagcgcg ccgatgccct ggatttccgg
     2401 cagcaggcgg aatccgggga agagtgcgag atgctcacgc cacaccagag cgtcccagaa
     2461 cccctcttcg ataaatgcga acagcaccaa caagccgatg gcgaacggca gcagcctgag
     2521 tgtgaacagt gaccaggggc gctgcagttg ctggtcgcgc cgtcgctgat agatgcaggt
     2581 cagcgccagg taggggacgc cgtgtgcgac cacattcgtg agcgtgaaca ccagatcgcc
     2641 gttggtgatg acgataccga agaaccagga caggcccgtg ccgagcacga tcgcattgcg
     2701 cggaatgttg aacggctgag cgcgaagccg aagccatgct tccttcgcca ggtaggcgag
     2761 acacagggcg acatagggcg gcagcgcgat gctccagaga atgtccggca gcggcaggaa
     2821 atcgccgtcg atgaaccata cgaactggcg gggcagatgc gtgtgccaat agagcagcgg
     2881 aaacagcgtt gctccgtaga tcgatatctg atcgagccgc cggcaccaga cgggcaagga
     2941 gcgctcggcg cgcgcgtaca gcattagaaa accatactgc tggcgtacga agtgaaacac
     3001 ggcggtatag gccagcactg tccagaagct cgcggcactc atcgagtaga gcagtacgcc
     3061 cgcgacccag gtagtgatgg ggacggcgat cagccagccg gacaggtgcg cgcgttccaa
     3121 cggatcaaag taagtgcgat acaccgtgct gtagacgtgg gcgacgtcga tgccaatgac
     3181 cagcagtgcc caactccaca ggcccaaacc ggcttggggc tggccgtcga ggacgatcag
     3241 cagtgcgacc gcggtggcga gcagcgcggg acccaagatg aacgtgccat cgatcgcggc
     3301 cgaatggatc cagggctgac gtaccgtggg cgcatgcagt gcgatcgtca tgcgagtgac
     3361 cgggccgcga gcgtaccggc ggactgaccg cgacagtagg cctcttcgaa aatcgaaatc
     3421 ccgctcaggt ccgagtgcgc aaagtagatc ggcggctgat gggcggtggc agaggcacgg
     3481 gcgtcgcccc agatgaatcc cggggtagga cgaatcatcg cgtgacccca cacgcgtacg
     3541 tcaatattct cgatgtgccc gtccagttcc ggatgcaccc gcagcagatc ggcgctcacg
     3601 aagccttgcc actgcgagag cgtgcgcttc aacgcagtgg tacgcgaggt ggctgggtcc
     3661 tgatccgaca gcggccagta gtaagtgatg acggtgtctc ggcgggcttg ggcgag
//
LOCUS       DATMRM010000488         4394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_1535693, whole genome shotgun sequence.
ACCESSION   DATMRM010000488 DATMRM010000000
VERSION     DATMRM010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535693"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2311
                     /locus_tag="VNZ06_14805"
     CDS             <1..2311
                     /locus_tag="VNZ06_14805"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW22067.1"
                     /translation="MTILKNGGASTLQVLDRIKAVLPAIRRQVPASLKVDLIGDQSLF
                     VRAAVNGVIREGLIAAALTGLMILLFLGSFRSTLIITISIPLSVLTSIFCLSAIGETI
                     NIMTLGGLALAVGILVDDATVAIENINWHLEQGKEVEAAILEGAQQISVPALVSTLCI
                     CIVFVPMFLLTGVSRFLFVPLAEAVVFAMLASYVLSRTLVPTLAKYWLHKHGPERIAA
                     GGRNLFVRLQHAFERAFAAFRTGYHELLESALHHRVGFTLVFMTIGATAFALLPWLGR
                     DFFPSVDAGQLRLHVRAVSGTRIEQTAILCDAVEREIRRVIPALELDNLADNIGIPYS
                     GTNLSYSTSAPIGPGDADILIALRAGRHRATADYVRALRPLLRERFPGTTFAFLPSDI
                     VNQILNFGLPAPLDVQVIGNDVEGNRAFTNRLLAKLKTIPGAADLRIQQDFAYPQLEV
                     NVDRTNAALVGLTQLNVATDVLISLSGSQQTAPTFWMDPKSGNQYSVTTQAPQYRLTS
                     LDDLAATPITTGNGGAQQLLANLATITRSVGPAVVSHYDSRPVIDIYGSVDGTDLGTV
                     ATAVRTMVDDARASLPRGSDIQVRGQIETMRSSFSGLAYGMIAAVVLIYLLIVVNFQS
                     WLDPLIIISALPAALAGMVWMLFVTHTTISVPALIGAIMCLGVATANSILVVSFAREQ
                     MNAGLTAAAAALRAGFVRFRPVLMTALAMVIGMLPMSLGLGEGGEQNAPLGRAVIGGL
                     LFATFATLLFVPVVFAVIHERFTPGKRLT"
     gene            2308..3462
                     /locus_tag="VNZ06_14810"
     CDS             2308..3462
                     /locus_tag="VNZ06_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWW22068.1"
                     /translation="MSEQPADHPNLGRLKRFALYVLIVAVIIAAWGILARISARAALK
                     SEAEEAAVAVVEVVQPRHSDGASNELVLPGDLQAYSDAPIYARTNGYLKRWYADIGKH
                     VKAGELLAEIETPEVDAQYRQAKADLASAQANNRLSQTTAARFRELRKTGLVAQQDAD
                     NAEGDADAKAAQVESAQQNLDRLQQLESFNHIVAPFDGVITVRRIDVGSLITQGNTTA
                     QELFHLSSTSRLRVYVQVPQAYASVIRPGMQAWLSTSERPDERHPVKVLSTAQAIDAA
                     TRTLLTELAADNPRGELLPGSYSQVHLPLPVGVDRWRVPATSLLYRGDGLHLAVVDAH
                     SHVHLHSVTIGKDYGAEIEILSGIEGQDRVIQSPPDSILEGAQVKVVGGN"
     gene            complement(3459..>4394)
                     /locus_tag="VNZ06_14815"
     CDS             complement(3459..>4394)
                     /locus_tag="VNZ06_14815"
                     /inference="COORDINATES: protein motif:HMM:NF042526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein"
                     /protein_id="HWW22069.1"
                     /translation="NYQVGVSIDVEPGQEAPEASSIFPVSAQPFTTQPVHGRPDIGAS
                     LQVRDLLNGAQPMPTPTGSTSSNSRIRPHGAFGQAPLTEDTGLLAFDSGVRSASNNLL
                     NPGYANRQAKVADRALETLAAIEAFCRGAGIDPRQLQVDSPARLLQLAGVLLREALVG
                     LKGLALSQREIREQAHVAVGKEELQHIGLTGLPVEDLMLRLLLGHESHDLDAVQWVRE
                     MLASTRRHDLATMSALNSALSDFISRLEPRSLLQSGARTNGAALTSADWAGLAARFRS
                     ITDAPGGTLPHLFLESFARAFIAVYRQSSSEPDKP"
BASE COUNT          733 a         1380 c         1452 g          829 t
ORIGIN      
        1 gatgacgata ctcaagaatg gcggtgcctc gacgctgcag gttctcgatc gcatcaaagc
       61 cgtgctgccc gccatccgcc ggcaggtgcc ggcgtcgctc aaggtcgatc tgatcggcga
      121 tcagtcactg tttgtgcgcg ccgctgtcaa tggtgtgatc cgcgaaggcc tcatcgccgc
      181 ggcgctgacc gggctgatga tcctgctgtt cctgggatcg ttccgcagca ccctgatcat
      241 caccatttca attccgctgt cggtgctgac ctcgatcttc tgcctgtcag cgattggcga
      301 gaccatcaac atcatgacgc tcggcgggct ggcgctggcg gtcggcatcc tggtggacga
      361 tgccaccgtc gcgatcgaaa acatcaactg gcatctggaa cagggcaagg aggtcgaggc
      421 cgcgattctc gagggcgcgc agcagatctc agtgccggcg ctggtatcga cgctgtgcat
      481 ctgcatcgtc ttcgtgccca tgtttctgct gactggcgtg tcgcgtttcc tgttcgtgcc
      541 gctggccgaa gcggtggtgt ttgcgatgct ggcctcctat gtcctgtcgc gcacgctggt
      601 gccgacgctg gccaagtact ggctgcacaa acacgggccg gagcgcatcg cggccggcgg
      661 gcgcaacctc ttcgtccgct tgcaacacgc tttcgagcgc gccttcgcgg cttttcgcac
      721 cggctatcat gagctgctgg aaagcgcgtt gcatcaccgt gtcggcttta cgctcgtgtt
      781 catgacgatt ggcgctaccg catttgcgtt gttgccgtgg ctcggacgcg actttttccc
      841 ctcggtcgat gccgggcagc tgcgcctgca cgtgcgcgcg gtcagcggca cgcgcatcga
      901 acagaccgcg attttgtgcg atgccgtcga gcgcgagatt cggcgcgtca ttccggcctt
      961 ggagctcgac aacctggccg acaacatcgg cattccctac agcggaacca atctttccta
     1021 cagcacctcg gcgccgatcg gtccgggtga cgccgatatc ctgattgcgc tgcgcgcggg
     1081 tcgtcatcgc gcgaccgcgg actatgtgcg ggcgctgcgc ccgctgctgc gcgagcgttt
     1141 ccccggcaca accttcgcgt ttctgccttc ggacatcgtc aatcagatcc tgaactttgg
     1201 cctgccggcc ccactggacg tgcaggtgat cggcaacgat gtcgagggca atcgtgcctt
     1261 caccaaccgt ctgctggcga agctgaagac cattccgggg gcggcggatc ttcgcatcca
     1321 gcaggacttc gcttacccgc agctcgaggt caacgtggat cgcaccaacg cggcattggt
     1381 cgggctgaca cagctgaacg tggccacgga cgtgttgatt tcattgtccg gcagccagca
     1441 gaccgcgccg actttctgga tggatccgaa gagcggcaat cagtacagcg tcacgacaca
     1501 ggcaccgcag tatcgcctga cctcgctgga tgatctggca gccacgccga tcacgaccgg
     1561 caacggcggc gcgcagcagc tgctggcgaa cctcgcgacc atcacccgca gcgtcggccc
     1621 ggcggtcgtg agtcactacg attccagacc ggtgatcgac atctatggtt cggtggatgg
     1681 caccgatctc ggaacggtcg ccacagccgt acgcaccatg gttgacgatg cacgcgccag
     1741 tctgccgcgc ggcagtgaca tccaggtacg cgggcagatc gagaccatgc gcagttcgtt
     1801 cagcggtctg gcctacggta tgatcgcggc cgtcgtgttg atctacctgc tgatcgtcgt
     1861 caatttccag agctggctcg atcccctgat catcatcagc gcgctgcccg cggcgctggc
     1921 gggcatggtg tggatgctgt tcgtcacgca caccaccatc agcgttccgg cgctgattgg
     1981 cgcgatcatg tgtctggggg tcgcgaccgc caacagcatt ctggtggtca gtttcgcgcg
     2041 cgaacaaatg aatgccggac tgaccgcggc cgccgctgcc ctgcgggccg gattcgtgcg
     2101 cttccgtccg gtgctgatga cggcgctggc gatggtgatc ggtatgttgc cgatgtctct
     2161 ggggctgggg gagggtggtg agcagaatgc gccgttggga cgcgcggtga tcggcgggct
     2221 gctgtttgcg acgtttgcca cgctgctgtt cgtgccggtg gtattcgccg tgatccacga
     2281 gcgcttcacg cccggcaagc gccttacatg agcgagcagc cggcggatca tcccaatctg
     2341 ggacgcttga agcgctttgc gctctatgtg ctgatcgtcg cggtgatcat cgcggcgtgg
     2401 ggaatcttgg cgcgcatcag cgcgcgcgcg gcactgaaat ccgaagcgga agaggccgcg
     2461 gttgcggtcg tcgaggtggt gcagccacgg cacagcgacg gtgccagcaa tgagctcgtg
     2521 ctgcccgggg atctgcaggc ctatagcgat gcgccgatct atgcgcgcac caacggctat
     2581 ttgaagcgct ggtacgctga catcggcaag cacgtcaaag ccggagagtt gctggccgag
     2641 atcgaaacgc cggaggtcga tgcgcaatac cgacaggcca aggcggatct tgcgagcgca
     2701 caggcgaaca accgcctgtc gcagaccacg gcggcgcgtt tccgcgaatt gcgcaagacc
     2761 ggactggtgg cgcagcagga tgccgacaac gcggaaggcg acgccgacgc caaagcggcg
     2821 caggtcgaat cagcacagca gaacctggat cgtctgcagc agctcgaatc cttcaatcac
     2881 atcgtggcgc cgtttgacgg cgtcatcacg gtgcggcgca tcgacgtcgg ttcgctgatc
     2941 acgcagggca ataccaccgc ccaggagctg tttcatctga gctccaccag ccgcctgcgt
     3001 gtctacgtgc aggtcccgca ggcctatgcc agtgtgatcc gtcccggcat gcaggcctgg
     3061 ctgagcacct cggaaagacc cgatgagcgc cacccggtca aagtgctcag caccgcgcag
     3121 gcgatcgatg cggccacccg cacgctgttg accgagctgg cggccgacaa cccccggggt
     3181 gagctgttgc cgggctccta ttcgcaggtg catctgccgc tgccggtggg cgtcgatcgc
     3241 tggcgcgtac cggccacctc actgctgtac cggggtgacg gtctgcatct ggcggttgtc
     3301 gatgcgcaca gtcatgtgca tctgcacagc gtgacgattg ggaaagacta cggcgcggag
     3361 atcgagatcc tgagcgggat tgaggggcag gaccgtgtga tccagagtcc gcccgactcc
     3421 atactcgagg gcgctcaggt gaaggtggtc gggggcaact agggcttgtc cggctcgctg
     3481 ctgctttgcc ggtaaacggc gatgaaggcg cgggcgaagc tctcgaggaa aagatggggc
     3541 agggtgccac cgggggcatc cgtgatgctg cgaaaacgcg ccgccagacc cgcccagtca
     3601 gccgaggtca gggcagcgcc attggtccgg gcgccgctct gcagcagcga gcgcggttcg
     3661 agcctcgaga tgaagtcgct cagggcagaa ttcagcgcgc tcatggtggc aaggtcgtgc
     3721 cgtcgggtgc tcgcgagcat ctcgcgaacc cactgtacgg cgtccagatc gtgactttca
     3781 tggccaagca gcagccgcag catcagatct tcgaccggca acccggtcag gccgatgtgc
     3841 tgcagctctt ccttgcccac tgcaacgtgc gcctgctcgc ggatctcgcg ctgcgacagc
     3901 gccaggccct tcaggcccac cagtgcctcg cgcaacagca cgccggcgag ctgcagcagg
     3961 cgcgcgggcg aatccacctg cagctgcctg gggtcgatgc cggcgccgcg gcagaacgct
     4021 tcgatggctg cgagggtctc cagtgcccgg tctgcgacct tcgcctgtcg attggcatag
     4081 ccgggattga gcaggttatt ggacgcggag cgtaccccgc tgtcgaaggc cagcagaccc
     4141 gtgtcttcgg tgagtggcgc ctggccaaag gcgccatgcg gacgtatgcg gctgttggag
     4201 ctggtcgagc cggtgggggt gggcatcggc tgtgctccgt tcagcaggtc gcgcacctgg
     4261 agcgaggcgc cgatgtcggg tcgtccgtgc accggctgtg tcgtgaacgg ctgggcgctt
     4321 accgggaaga ttgaactggc ctcgggagcc tcctgacccg gctcaacgtc gatgctgacc
     4381 cccacctggt aatt
//
LOCUS       DATMRM010000489         7331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3372353, whole genome shotgun sequence.
ACCESSION   DATMRM010000489 DATMRM010000000
VERSION     DATMRM010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3372353"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1177
                     /locus_tag="VNZ06_14820"
     CDS             176..1177
                     /locus_tag="VNZ06_14820"
                     /inference="COORDINATES: protein motif:HMM:NF016491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HWW22070.1"
                     /translation="MGFFQTFWTWLNAQLASYISDNTARVASAIEPAVIALATVYIMA
                     WGYLHLTGRIDEPFSTGLSRVIRLTVVIGVSLHLWLYNDVIVDTFYRAPSQLANAVIG
                     ASDPVDTVDAIWNRGGAVADVLWHKQGFGTIGSSIMGAVVWLMVGLLCVYVMFLISLS
                     SIACAVLLAIGPLFIALCLFDTTRRFFEAWIAQLANYALITVLSVLVSALLLHLVESY
                     AAQTAALSTALNMVDALDMLLMAGLVLLLMRQIMPIAAGLAGGIALSSFNSVSRSLAW
                     ARPASRTLGGLAVAGSRLFTKTIYTDVGGGANAGRAASSEGAISTAEAANALSGRDL"
     gene            1314..2054
                     /locus_tag="VNZ06_14825"
     CDS             1314..2054
                     /locus_tag="VNZ06_14825"
                     /inference="COORDINATES: protein motif:HMM:NF016238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HWW22071.1"
                     /translation="MSAQPRSGSLDAYFAEASEWDRDRRDGLRREVRIAWCITIGAVI
                     CAIAASVALLLAMPLKRVDPFLIRVDRSSGVVDVVPIFAGEAPLDESVTRYFLTHYVS
                     VCERFNFSTAESDYEECGAFHSAQRNQSWYALWNRSNPRSPLNVHRDGSAVEAQVSAV
                     SFFTRASGISDTAQVRYLKLERAAPGAPERITHWIATIQYAYTTPATDPKVRRWNPLG
                     FRVLDFISEAEVTGEIAASPSPKSEVAP"
     gene            2051..2830
                     /locus_tag="VNZ06_14830"
     CDS             2051..2830
                     /locus_tag="VNZ06_14830"
                     /inference="COORDINATES: protein motif:HMM:NF015487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HWW22072.1"
                     /translation="MRLAICVACTVVSLPVMAVDSRLRSVPYSADEVYRLHGVAGYQI
                     DLEFDPDERFVGIGSGDVEGLAFEAQGNHLFIKPRAINVRTNLTVLTTRRTYHFEYYS
                     SPARTEIDSQDLIYALRFVYPKPESVPATAGAAIDASLSQASPRHEHNTDYWYCGPGV
                     LQPIEAWDDGVHTHLHFSSRIELPALFVRNDDGSESLLNFNVEHDEVVIHRIARRFTV
                     RRGALAGCILNRGFSGTGDALDTGTVSPEVERVIRSSAGAP"
     gene            2896..3984
                     /gene="virB10"
                     /locus_tag="VNZ06_14835"
     CDS             2896..3984
                     /gene="virB10"
                     /locus_tag="VNZ06_14835"
                     /inference="COORDINATES: protein motif:HMM:NF038091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB10"
                     /protein_id="HWW22073.1"
                     /translation="MLSLQSRLSHVLAIALMLAMGAVFLCWYYAHMLSRPSRMRQNAQ
                     SSLAAKASGEMSLPSLGQLDLPQTPPSPAPTQARPVPVPSALLMPATSETAVPAASAV
                     MGGPAVHATALERRLSGAAFASGGRDASPAASGVAAPRPTQGEAQSSDTLSTLLASDS
                     TSVVSARWVAAQRMLLPKGAFIDCTLETAIDSSLPGLTTCVTATDTFGADGRVVLLER
                     GTKLVGETRGQLQQGAARVFVLWTEARTPAGVRVPLDSPGSDELGRSGLPGQVDRHFW
                     ERFGAAILISTLDGGVQAAVQSASRGSGTVIYSPSGPQDVTSEVLKGTLGIAPTVIKH
                     NGDRIQILVARDVDFSRVYELRHAVAEP"
     gene            3965..5020
                     /gene="virB11"
                     /locus_tag="VNZ06_14840"
     CDS             3965..5020
                     /gene="virB11"
                     /locus_tag="VNZ06_14840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HWW22074.1"
                     /translation="MPSLNPETLVADRVSKAPEHTALALMLEPLRGFLRNSAITEICI
                     NRPGGVFIETHAGWQHELVRELDLNWCHRFAKLVANHTRQRVDSTSPLLSASLPSGER
                     VQLVLPPATTPGSVAIAVRRPADQLWSLSELAQRGLFRATRRADASHSRLETELSRLL
                     SAGQFEAFLSLAVRSRLNIVVSGPTGSGKTTCTKALIREIPAAERLITIEDARELVLD
                     RHLNHVRLFYSKDDQGLARVTPKQLIECCLRMKPDRILLAELRGEEAFDYLRSVNCGH
                     PGSITSVHATSAELAFEQMVLLVKQSQAGRELSRADIKGLLHLSIDVVVQFDARAHER
                     FVKEIWYEPSLKHRAQR"
     gene            5031..6821
                     /locus_tag="VNZ06_14845"
     CDS             5031..6821
                     /locus_tag="VNZ06_14845"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HWW22075.1"
                     /translation="MDLVHGWGSWWLGYAGVQAPMVFAMARAKASMNRLLLSPLAAVP
                     ATLAASVGFSIVSPGLSGAGFDAGSLMQLATGMGIAIGTGYLSGRLLSRPTVDSRIDM
                     RGSSVSRDRGWNEAKEAVRAGELALAGARVAAADETKHFKFIGTTGTGKSTAIARMLE
                     RALARGDRVVIADPDGGYLRRFYNAERGDVILNPFDQRSVKWDLFGEIRQQYDVDQLA
                     RSLIPDHQGDDRSWRGYGRTFFSAVARQAHAMGVRDVGELYRLLTVADSAELASLTRG
                     TPAQPFLEPSNAKMFHSLRSVTSSAVASLEYVANQRSSPFSIRNWVDQERKDTGGALF
                     IPYRAGQIAALRSTISAWVRIAIFAAMDRPEGDQRLWFVVDELDALGPIDGLKDALAR
                     VRKFGGRCVLGFQSVAQVASTYGDGEAHTIVENCGNSFILRCSASEGGGTARFASVLI
                     GQREVRRTTLSRSTRDTELFGSSTTSDHLSIEPAVMDSEIEQIPDLCGYLKLASRADW
                     LRVRMPVPAPRTAASPETHAFVPAPVEPPSMGRTAIPESNTPSPDFAAASAKPRRRAR
                     TKVAPSEALTDASAPAKRPRRAAQTPGFEP"
     gene            6818..7330
                     /locus_tag="VNZ06_14850"
     CDS             6818..7330
                     /locus_tag="VNZ06_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22076.1"
                     /translation="MTDLPAADPDTLKLGMLLEAAQSHQELADDSLKRLQAHTQGLDG
                     VVRDEIRRALIAELGDLVEHLNRAAYSLRALSRVAQLRTVWCGAVLSACPALVVSLLV
                     WWWLPSGDQISKLRIQQQQFADNLEYLRRNGAQIDLRHCGNPARLCVRVDRQAGAFGQ
                     QADYLIVQGY"
BASE COUNT         1286 a         2221 c         2374 g         1450 t
ORIGIN      
        1 gccgagcagg gcatgcttca gaacgagcag accaagctgc agaccctgta tcaggcggtg
       61 cagtcgcagg ccgaggtctt gagacagcag gcccaggagc aggcgatcgc cgactacggc
      121 aacttttcta cgcgctttga gccgacaccc tagagcgctt agctaccgaa cgctcatggg
      181 attcttccag acattctgga cgtggttgaa cgcgcagctg gccagctaca tcagcgataa
      241 cacggcacgt gtggccagcg ccatcgagcc ggccgtcatt gctctcgcga cggtgtacat
      301 catggcctgg ggctacctgc acctgacggg tcgcatcgat gaaccatttt ccacgggtct
      361 cagccgcgtc atccgcctga cggtggtgat cggggtgagc ctgcacctgt ggctctacaa
      421 cgatgtcatc gtggatacgt tctatcgcgc gccgtcgcag ctcgcgaacg cggtgattgg
      481 cgcgtccgat cccgtcgata ctgttgacgc gatctggaac cggggcggcg cggtcgcgga
      541 cgttctctgg cacaagcagg ggttcggcac aatcggttct tccatcatgg gagccgtggt
      601 ctggctgatg gtcggattgc tgtgcgtcta cgtgatgttc ctgatctcgc tgtcgagcat
      661 tgcctgcgcg gtgctgttgg cgatcggtcc cctgttcatt gcgctgtgct tgtttgatac
      721 cacccgccgc ttcttcgaag cctggatcgc gcagctcgcc aactacgcgc tgatcaccgt
      781 tctgagcgtg ctggtatccg cactgctact gcacctcgtg gagtcctatg cggcgcaaac
      841 cgctgcgctc agtaccgcgc tgaatatggt tgatgcgctc gacatgctgc tgatggcagg
      901 tctggtgttg ctgttgatgc gtcagatcat gccaatcgcg gcgggactgg ccggtggcat
      961 tgccctcagc agcttcaatt cagtcagccg ctcgcttgcg tgggccagac ccgcgtccag
     1021 aacgctgggt ggactcgctg tcgcggggtc ccgtttgttt accaaaacga tctacaccga
     1081 tgtcggtggc ggcgcgaatg ccgggcgggc ggcgagctcg gagggcgcca taagcactgc
     1141 ggaggcggcc aacgcacttt ccgggaggga cctgtgacga ggaggcactg gatggtggta
     1201 ttgacggtgt tggtcctgtg cggctgcgtg gtccgggagg atcggtgcca tcgcgcgctg
     1261 aggcagatca atccgccggc tgcgcatcag ccgacgccgc ccgaggcgag cccatgagcg
     1321 cgcaaccgcg ctcggggagc ctggacgcct atttcgccga ggccagtgaa tgggaccggg
     1381 accgtcgaga tggtctgcga cgcgaggtgc gtattgcatg gtgcatcacg atcggcgctg
     1441 tgatctgcgc aatcgccgcc agcgtggcgc tgttgctcgc gatgccgctc aagcgggtcg
     1501 acccgtttct gattcgcgtc gatcgcagca gcggagtcgt cgacgtcgtt ccgatctttg
     1561 ccggcgaggc gccgctggat gagagcgtca cgcgctattt tctcacgcac tatgtcagcg
     1621 tctgtgagcg tttcaatttt tcgaccgcag agagcgacta cgaggagtgc ggcgcctttc
     1681 acagtgctca acgaaaccag tcctggtacg cattgtggaa ccggagcaat ccgcgttcac
     1741 cactcaacgt gcaccgggac gggagtgcgg tcgaggcgca ggtctccgcc gtcagcttct
     1801 ttacacgcgc gagcgggatc tcggacaccg cgcaggtgcg atacctgaag ctcgagcgtg
     1861 cggctcccgg cgctcccgaa cggatcactc actggattgc gacgatccaa tatgcctaca
     1921 cgacgcccgc cacggatccg aaggtacgcc gatggaaccc gctgggcttc cgggtgctcg
     1981 atttcatctc ggaagcggag gtcaccggtg aaatcgctgc gtcgccatcg ccgaaatccg
     2041 aggtcgcgcc atgagactgg caatctgtgt ggcctgcacc gtcgtgtcgt tgccggtaat
     2101 ggcggtggac agccgcttga ggtcagtacc ctacagcgcc gatgaggtct atcgcttgca
     2161 cggtgtcgcc ggctatcaga tcgatctgga gttcgatccg gatgagcgct tcgttgggat
     2221 cggcagtggc gatgtcgaag ggcttgcgtt cgaggcgcag ggcaatcatt tgttcatcaa
     2281 gccccgggcg atcaatgtca ggaccaatct gacggtgctc acgacacgtc gtacctatca
     2341 cttcgaatat tactcatcgc cggcgcgcac cgaaattgat tcccaggacc ttatctatgc
     2401 cctgcgcttc gtctatccca agccagagag tgttccggct acagccggtg ccgccataga
     2461 tgcctctttg tcgcaggcct cgccccgaca tgagcacaac accgactact ggtattgcgg
     2521 gcctggagta ttgcagccga ttgaagcgtg ggacgatggg gtgcacacgc acttgcactt
     2581 tagttcacgc atagagctgc ctgctctctt tgtccgcaac gatgacggca gcgagtcact
     2641 gctcaacttc aacgtcgaac acgacgaggt cgtcattcat cggatcgccc gccggttcac
     2701 cgtgcggcgc ggggcgctgg cgggatgcat cctcaatcgg gggttttcgg ggaccggaga
     2761 tgcgctggat acgggcaccg tgtcgccaga ggtcgagcgc gtgatccgct cgtcggcagg
     2821 cgcgccctga tgttgctgga taccacagcg cctgcagctc tgcgcggaga tcgcggcaaa
     2881 ccactcgtcg cgagaatgct atcactgcag tcgcgcctca gccatgtcct ggccattgcg
     2941 ctcatgctcg ccatgggcgc ggtatttctg tgctggtact acgcacatat gctcagtcgg
     3001 ccatcgcgca tgcgacagaa cgcgcaatca agcctcgctg ccaaggcgag cggggagatg
     3061 tcgctgcctt cgctgggaca gctcgatctg ccccaaaccc cgccgtcgcc agctcctacg
     3121 caagcgcgcc cagtgccggt gccgagcgcg ctcttgatgc cggcaacgtc tgagaccgct
     3181 gtgcccgccg ccagcgcggt gatgggcggt ccggcggttc acgccacggc gctggagcgc
     3241 agactatctg gtgcagcctt cgcctcgggt ggacgcgatg cgtcgcctgc cgcctctggc
     3301 gttgcagcgc ctcggccgac tcaaggagag gctcagtcga gcgacacgct atcgacgctg
     3361 ctggcgtcag attccacttc cgtcgtgagc gcgcgctggg tagccgccca acgcatgttg
     3421 ctaccgaagg gagcgttcat tgactgcacg ctggagacgg cgatcgattc ttcgcttccc
     3481 ggactgacga cctgtgtgac ggccaccgac accttcggcg ctgacgggcg ggtcgtgctg
     3541 ttggagcggg gtaccaaact ggtgggtgaa acacgcggac agctccagca gggggctgcg
     3601 cgcgtgttcg tcctttggac ggaagctcgc acgcccgccg gagtcagggt gccgttggat
     3661 tcgccagggt cggacgagtt gggccgatcg ggcctgcctg gccaggtgga ccgacatttc
     3721 tgggagcgct tcggtgccgc cattctgatc tcgacgctcg atggcggcgt ccaagccgcg
     3781 gtgcagtccg cgagccgagg cagtggaacg gtgatttaca gtccgagcgg tcctcaggat
     3841 gtcaccagcg aggtgctgaa gggaacgctg ggcattgcac cgacggtcat caagcacaac
     3901 ggcgatcgta ttcagatcct ggttgcccgc gacgtggact tcagtcgcgt gtacgagttg
     3961 cgacatgccg tcgctgaacc ctgagacgct cgttgccgac cgggtttcga aagctccaga
     4021 gcatacggct ctggccttga tgctcgagcc gttgcgcggc tttctgcgca actcggcgat
     4081 cacagagatc tgtatcaatc gtccgggtgg ggtgttcatc gagacacacg ctggctggca
     4141 gcacgaattg gttcgtgaac tcgacctgaa ctggtgtcat cgcttcgcca agctcgtggc
     4201 caaccacaca cgccagcgag tggattccac ctcgccgttg ctttcggcct ctcttcccag
     4261 tggtgagcgc gtgcaactgg tgctaccacc agcgacgacc cccgggtcgg tggctatcgc
     4321 cgtgcgacgc ccggccgatc agctctggtc gctgtcggaa ttggcgcagc ggggattgtt
     4381 tcgcgcgacg cgacgggccg acgcgagcca cagccgattg gaaactgaat tgtcgagatt
     4441 gctgtcggcg ggtcagttcg aagcattcct aagcctggcg gttcgcagcc gactgaacat
     4501 cgttgtctca ggtccaaccg gctccggcaa aacgacctgt accaaagcgc tgatccgcga
     4561 gatccctgcc gccgaacgac tcatcacgat cgaggatgcc cgcgaactcg tgctcgaccg
     4621 ccacttgaac cacgtgcgcc ttttctattc gaaggacgac caggggctgg cgcgcgtgac
     4681 acccaagcag ctgattgaat gctgcctgcg aatgaagcct gatcggatcc tgctcgcaga
     4741 gttgcgaggc gaggaggcct ttgactacct gcgaagcgtc aattgcgggc atcccggatc
     4801 gatcaccagt gtgcacgcaa ccagcgcgga gctggccttc gagcagatgg tgttgctcgt
     4861 caaacaaagc caggcgggca gggaactctc gagggcggac atcaagggac tgttgcattt
     4921 gtcgatcgac gtggtggtgc agttcgatgc ccgggcgcac gaacgcttcg tcaaggagat
     4981 ctggtacgag ccctccttga aacatcgcgc ccaacggtag ctgcgcgtat atggacttgg
     5041 tacacgggtg gggcagctgg tggttgggct acgcgggtgt gcaggcgccg atggtgtttg
     5101 cgatggcgcg cgcgaaggcc tccatgaacc gactgctgtt gtcacccttg gccgccgtgc
     5161 cggcaaccct ggcagccagc gttggctttt ctattgtgag tccagggcta tccggcgcgg
     5221 gatttgatgc tggcagcctg atgcagctgg caacgggaat ggggattgcg atcggtaccg
     5281 gttatctgtc tggccgacta ttgagccgtc ccacagttga ctcgcggatc gacatgcgcg
     5341 gcagcagcgt cagccgcgat cgcggctgga acgaggccaa ggaggcggtg cgagccggag
     5401 aactcgcgct tgccggtgcg cgggtggcgg ccgcggacga gaccaagcac ttcaagttca
     5461 tcggcacgac cggcacgggg aaaagtacgg ccatcgcgcg aatgctcgag cgtgccctcg
     5521 cgcgtggcga tcgcgtcgtg attgcagatc ccgatggcgg ctatctgcgt cggttctaca
     5581 acgcagagcg cggcgatgtg attctcaatc cgttcgatca gcgctcagtc aagtgggatc
     5641 tgttcggcga gattcgtcag cagtatgacg tggatcaact agcacggtcg ctgatacccg
     5701 atcatcaggg cgatgaccga agctggcgcg gctatgggcg aacgttcttt agtgcggtcg
     5761 cgcgtcaggc acatgccatg ggtgtgcggg atgtcggcga actatatcga ctgttgacgg
     5821 tcgctgacag cgctgagctt gcgagcttga cccgcgggac gccagcgcag ccgtttctcg
     5881 agccgagcaa cgccaagatg tttcattcgt tgcggtcggt caccagctcc gcggtcgcat
     5941 cgctggaata tgtcgcgaat caacgctcgt cgccgttttc gatccgcaac tgggtcgatc
     6001 aggagcgcaa agacaccggc ggcgcgctct tcattccgta tcgcgcgggc cagatcgcgg
     6061 cgctgcgctc aacgatctcg gcctgggtgc ggatcgctat ttttgcggcg atggatcggc
     6121 ccgagggcga tcagcggctg tggttcgtgg tcgatgaact ggatgcacta ggaccaattg
     6181 atgggctgaa ggatgcgttg gccagagtgc gcaaattcgg aggacgctgc gtgttggggt
     6241 ttcaatcggt cgcgcaggtc gcaagtactt acggtgatgg tgaggcacat accattgtcg
     6301 agaactgcgg caatagcttc attctacgct gttcggccag cgaaggaggc ggcactgcgc
     6361 gtttcgcatc ggttctgatt ggtcaacgcg aggttcggcg aacgacactc tctcgcagta
     6421 cgcgtgacac ggaactgttt ggttcgtcga cgaccagcga tcatctcagc atcgagccgg
     6481 ccgtgatgga ctccgagatc gaacagattc cggacctatg cggctatcta aagctggcga
     6541 gtcgcgccga ctggcttcgc gttcgaatgc cggtacccgc gccacgcacc gcagcgtctc
     6601 ccgaaacgca cgcgttcgtc ccggcaccgg tcgagccgcc cagcatgggt cgtaccgcca
     6661 tccctgaatc gaacacaccc tcgccggatt tcgcggcggc gtccgccaag ccgcggcgtc
     6721 gcgcgcggac caaggtcgca ccctcagagg ccctcaccga cgcatcagcg ccggctaagc
     6781 gtccacggcg cgccgcgcag accccgggat ttgagccgtg accgatctcc cggcggccga
     6841 tcccgatacc ctgaaactcg gcatgctcct ggaggctgcg cagagccatc aggagctcgc
     6901 ggatgacagt ctgaagcggc tgcaggcgca cacacagggt ctcgatggtg tcgtgcgcga
     6961 cgaaatccgc cgcgcgctga tcgcggagct tggagatctc gtcgagcatt tgaaccgcgc
     7021 agcctattcg ctcagagctc tcagccgggt ggcacagctg cgcacggtgt ggtgcggagc
     7081 cgtgttgtcc gcctgtccag cgctggtggt gtctttgctc gtctggtggt ggctaccgtc
     7141 cggcgatcag atcagtaaac tccgcatcca acaacaacag ttcgccgaca acctggaata
     7201 cctgaggcgc aacggtgcgc agatcgatct gcgccactgc ggcaatccgg cgcgcctttg
     7261 tgtccgagtg gaccgccagg caggtgcctt cggtcaacag gccgactacc tgattgtgca
     7321 gggctactga a
//
LOCUS       DATMRM010000490        13571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2768872, whole genome shotgun sequence.
ACCESSION   DATMRM010000490 DATMRM010000000
VERSION     DATMRM010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13571
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2768872"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..106
                     /gene="rpsR"
                     /locus_tag="VNZ06_14855"
     CDS             <1..106
                     /gene="rpsR"
                     /locus_tag="VNZ06_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HWW22077.1"
                     /translation="PSRITGTKSFYQRQLATAIKRARFLALLPYTDQH"
     gene            118..573
                     /gene="rplI"
                     /locus_tag="VNZ06_14860"
     CDS             118..573
                     /gene="rplI"
                     /locus_tag="VNZ06_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HWW22078.1"
                     /translation="MELILLQKVANLGNIGDRVKVKSGYGRNFLLPKGKATLATAANV
                     ARFEARRAELEKLANDELTSAQQRAAALKEFKLTITAKSGTEGKLFGSVGTADIAEAV
                     TAAGHTLARSEVRLPGGPLRALGEHSVHIHLHADVDLELPVIIVAEEAG"
     gene            589..2628
                     /gene="dnaB"
                     /locus_tag="VNZ06_14865"
     CDS             589..2628
                     /gene="dnaB"
                     /locus_tag="VNZ06_14865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HWW22079.1"
                     /translation="MSTPLRSRRDGGATGAEGLVPPQSVEAEQSVLGALLIDTHAWDQ
                     VADIVTAADFYRPDHRMIFEALGELVAAGRPGDVVTVSEQLERHGRLLDAGGLAYLGT
                     LARDTPTAANARSYAQIVRERALLRRLLEAGREIAASVFDEEGLSARELVDNAEKRVF
                     EIAEMGSRGRDGAVRVSALLPPLIDKIDEWHTNPNKLRGLATGFTDFDRKTGGLRGGD
                     LIIIAGRPSMGKSTLAVNMAEYAAINPEIRARVAIFSMEMPSEQIVTRMLASIGHVPL
                     NSMRTGQISDDDWVRITSATSQLSEARIFIDETPALTPTELRARSRRIAREQGGLDLI
                     VIDYLQLMQVQGNTENRATEIAEISRGLKALAKELDVPVIALSQLNRGVEQRQEKKPV
                     MSDLRECVTADTLVCLTDGRRVPIQELVGTAPEVWSLNASQRLHAARADKVWHVGRKS
                     VFRVELASGRNIRATAEHRLLAGAGWMTVGELKLGERLAVARRIPPAAKLASRPDNWL
                     MAGVGAWLKRVSAPMQGQRISRTQMANIRRRAVLDTQSAPSGAPVSEYAHILGDEKPH
                     DLARNDIFWDRVTAIIAEGDEEVYDLTVPGPANWLADGVITHNSGAIEQDADMILLIY
                     RDEVYDKNTTKKGMAEIDLAKHRNGEIGPFLLTFQGQYTRFVNYAPDTYAEGVLR"
     gene            2628..3701
                     /gene="alr"
                     /locus_tag="VNZ06_14870"
     CDS             2628..3701
                     /gene="alr"
                     /locus_tag="VNZ06_14870"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWW22080.1"
                     /translation="MRLIRARIDSAALQANLKTIRSHAPNSRVMAVVKANAYGHGLVP
                     SALGLIDADAFAVARLEEAIALRTAGLKHRIMLLEGVFDAAQLAEAARHHLDLVIHQP
                     LQIELLESHRGSQRWCLWLKVDTGMNRLGFRPEQFHDAWRRLQALHTAPMELRVLTHL
                     ACADEADESITARQLARLAPLIAGLDVELSIANSAGILAQPSTHSAWVRPGLALYGIS
                     PFPSRLGSQLGLKAVMTLESRVIALRDVLPGEQVGYGGVWTARRRTRVAIVAAGYGDG
                     LPRSLPNGAPVLIGGHRGSLVGRVSMDMVAVDVTDLPPIPIGERVELWGPHLAVEELA
                     RAAGTIPYELVCGVSQRVPIELV"
     gene            3711..5492
                     /locus_tag="VNZ06_14875"
     CDS             3711..5492
                     /locus_tag="VNZ06_14875"
                     /inference="COORDINATES: protein motif:HMM:NF002505.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClcB-like voltage-gated chloride channel
                     protein"
                     /protein_id="HWW22081.1"
                     /translation="MEALVQFGTRLYDRVERLVRAGLIAARHRELSGIVFWAALIGIC
                     GAFTGVAFRASVRLVQRLLTGSSAGLVETAELLPWWARIMVPTIGGICAGTLLWAGQR
                     ILRQSRTVDYMEAVAVGNGQLSSRASLLQATSSFFTIGSGGSIGREGPLVQLAATVAS
                     KIGQWTRAPVPRLRLLVACGASAGIAAAYNAPIAGALFVAEVVLGSIAMESFGPLIVA
                     SVVSDATTRHFLGYGPVYLVPHINFGATWELALYALLGIFLGHAAPPYLALLDNARRR
                     FRRLRWPLPVTLALGGLVVGAISVGVPQVWGNGYSVVNDMLNDKLSLQLLGLVVVAKL
                     ISTTASVGSGANGGILTPTLFMGAAFGALYAHGLHFAHPQLSSQIAAYAVVGMGSFLA
                     ATTHAPLTSILLIFEMTLDYEVVLPLMLACVTGHYVARIYRQGESVYTRALKAKGPGE
                     KEEWNLRQITALIRPASVQVHRDSLLGNAIDRPPGSANSAYVVDSQNQLVGQLDIAGV
                     KRQLRTTPAGPLALVATYMQPAPPVLTPEMLLGDALDVFLAYRAKQLPVVSGQWSPIL
                     IGEVSRHDLLLALQDRMAERPNQVPAH"
     gene            complement(5505..6200)
                     /locus_tag="VNZ06_14880"
     CDS             complement(5505..6200)
                     /locus_tag="VNZ06_14880"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWW22082.1"
                     /translation="MARLILSLDGAVLAEYNMNKERYTIGRLPDNDIRIDNPAVSGHH
                     SLIINILNDSFLEDLNSTNGTYVNGKLIKKHALQHGDVITAGHHQLRFVEEDEAAQDD
                     FEKTMVIQPSARPVDKVRAATTDTSATGARRAIANGAPALRKAKLQVLSGAFAGRELE
                     LTKALTTLGRPGVQVAAITRRSEGFFIVHVDAEQADNFPLVNGAPIGAQARKLADNDV
                     IQLAGVKMGFFEG"
     gene            complement(6211..7041)
                     /locus_tag="VNZ06_14885"
     CDS             complement(6211..7041)
                     /locus_tag="VNZ06_14885"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HWW22083.1"
                     /translation="MSLKGKVQVVGQTDTGRVRDHNEDTIAYDPDIGLMVLADGMGGY
                     NAGEVASGIAVKTIVGLVRESVEREDLRVHDSEAGLTRASIILRDAIVRANKIIFQTA
                     RSQPQCEGMGTTVVGALFFDNRISIAHVGDSRMYRLRQADLEQITMDHSLLQELVDRG
                     FYSPEEAQRAANKNYVTRALGVEQNVEVEVQEHPVQKGDMYLICSDGLSDMVEDDDIH
                     LTISTFGANLETATKQLIQLGNDNGGRDNISVVVAEILESFPAPRGIFDKMLGWFSST
                     "
     gene            7237..8589
                     /gene="radA"
                     /locus_tag="VNZ06_14890"
     CDS             7237..8589
                     /gene="radA"
                     /locus_tag="VNZ06_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HWW22084.1"
                     /translation="MARTQTLYVCRSCGGEAVKWQGQCTHCKEWDSLEAVTAVRGVRQ
                     MTASEAVAGPLQDATENTASAVRHSFGFSELDRVFGGGLVSGSVTLLGGEPGIGKSTL
                     LLQVAAALSNQQKTLYASGEESTAQISLRARRLQLNAPQLALLSEHSLDAILGLAAAR
                     KPDLLIVDSIQTMQLAANEASAGSPAQLRECAALLVRFAKANACAVIIVGHVTKDGSI
                     AGPKLLEHLVDTVLYFESDAGSRYRMLRATKNRFGPVNELGFFAMSDAGLKEVRNPSA
                     IFLSRHPEPVPGSVVMVARDGGRPMLVEVQALVSRAGFGAPRRVAQGVDSNRVSMLLA
                     ILSRHADLALADYDVFVNLVGGLDIAETATDLPLALALASSLKDQPVRESLAAFGELG
                     LTGEVRPVAYGDERVREAVKLGFSRVMLPRENLPRTPPEAVSLQPVSRIADALSLAFA
                     "
     gene            complement(8595..9869)
                     /locus_tag="VNZ06_14895"
     CDS             complement(8595..9869)
                     /locus_tag="VNZ06_14895"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HWW22085.1"
                     /translation="MDKSPTIVLILILFGLLLVIAFSSGTEVAMLSVNRYRIRHRAEL
                     GERRARLLEELLAQPDRWLGANLVILALASVAASTVATLLALRSALPHAIVFELVLLT
                     VVVIVFCELAPKIFAAVHAEGVALRCAYVYRLLLWITWPVTWLANYVAQGFLRLFGVT
                     RSSAGGNGLSADELRTVVNEAGALIPARHRQMLLSILDLERATVNDIMIPRQEIAGID
                     ADVSWEDILDRLRQTPHSRLPVYHRELDSLVGTLHMKRVAQELARGSLTRERMLEIAA
                     AREPYFVPEGTTLTQQLAQFQRTRRRLAFVVNEYGDVAGLVTLEDILEEIVGEFTNDP
                     ATVTHKDVSRDAAGNWLVNASATIRALNRSLGWQLPDIGPRTLNGLLLEKLETIPPPG
                     TALRIGDYDFEVLQIADNAIRTVRVRAASKDR"
     gene            9982..11346
                     /gene="ffh"
                     /locus_tag="VNZ06_14900"
     CDS             9982..11346
                     /gene="ffh"
                     /locus_tag="VNZ06_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HWW22086.1"
                     /translation="MFDKLTERLGRAVQTLRGRGRLTEENIAETLREVRMALLEADVA
                     LPVVKTFIDSVKAEALGAEVAASLTPGQAFIGILHRQMVALLGQSQAGFALNVQPPQV
                     VLLAGLQGAGKTTTAAKLARWLRESHRRRVLLVSTDVRRPAAILQLQRLSESVGADFF
                     PSDSSQAPAAIALAALDAARRGVYETLIIDTAGRLHVDAELMEEVRQIDRAVSSHQRL
                     FVVDSMAGQDALNAARAFSSALELTGIVLTKADGDARGGAALSVRHVTGKPIVFLGVG
                     EKVDALQPFDPERMATRILGMGDVVSLVEEVTRKVDVDEAQKLARKVVSGKAFTLVDL
                     RSQLEQVRKLGGLSAVMDKLPSQLAGKAAVKGDQADRELKRQIAIIDSMTRQERRQSG
                     IIDGSRRRRIASGAGVQVQDVNRLLKQFMDMQRLMKNMKGGKLARMMAGLKGGIPPGM
                     PPRR"
     gene            11434..11703
                     /gene="rpsP"
                     /locus_tag="VNZ06_14905"
     CDS             11434..11703
                     /gene="rpsP"
                     /locus_tag="VNZ06_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HWW22087.1"
                     /translation="MVTIRLTRRGGTNQPFYHVVVTDSRERQGGSSLELVGYFNPVAR
                     GKETRLKLDLDRIDHWMKHGAQTSERVRHLVAGYRKEAAKQVAAA"
     gene            11718..12200
                     /gene="rimM"
                     /locus_tag="VNZ06_14910"
     CDS             11718..12200
                     /gene="rimM"
                     /locus_tag="VNZ06_14910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HWW22088.1"
                     /translation="MGRIIGPHGVRGALKVLSYAHPPQSLLQYRSWRLRRADGGEDEY
                     RVLQSQWDGHLMRAQLEGVADRDAAQRLREREILIERDLMPAAGPGEYYREDLQGFTV
                     RNLEGVVLGTLQYFLEAPAGALMVVSGERERWLPTTAPFLKRVDLERREIELDWPADF
                     "
     gene            12203..12958
                     /gene="trmD"
                     /locus_tag="VNZ06_14915"
     CDS             12203..12958
                     /gene="trmD"
                     /locus_tag="VNZ06_14915"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009683443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWW22089.1"
                     /translation="MHIEVVTLFPGLIQEALRYGVLGRAIERGLLQVETVDPRSFTHD
                     AHRSVDDRPYGGGPGMVLTPEPLCAAIAAAAERSPPGSVRVALSAQGELLNQARVAQW
                     SQRPGLILIAGRYEGIDERVLQLSVDQEISIGDYVVSGGEMPALVLIDALARLLPGAL
                     GCARSNVEESFDTGLLDWPHYTRPEIFRGQRVPEVLSSGDHAAIRRWRMKQSIGRTWL
                     RRPELIGKTPLDTERRVLLEEFLMEQLAKAADN"
     gene            12969..13313
                     /gene="rplS"
                     /locus_tag="VNZ06_14920"
     CDS             12969..13313
                     /gene="rplS"
                     /locus_tag="VNZ06_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HWW22090.1"
                     /translation="MSNIIQTLEAERMTRKVPAFKPGDTVVVQIKVVEGDRERVQAYE
                     GIVIARSNRGFNSSFTVRKVSHGEGVERTFPIHSPSISEISVKRRGSVRRAKLYYLRD
                     RAGKAARIEEKV"
     gene            13323..>13571
                     /locus_tag="VNZ06_14925"
     CDS             13323..>13571
                     /locus_tag="VNZ06_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22091.1"
                     /translation="MHALGRIIYGVWRGLDVLRRFLHLLVLLALFGILVGALRQVTPR
                     VPEKAALVVRPSGDLVEQISGEPLARALSEAQGGGMPQT"
BASE COUNT         2400 a         4101 c         4518 g         2552 t
ORIGIN      
        1 gcccagtcgc atcaccggta ccaagagctt ctatcagcgt cagctcgcca ccgcgatcaa
       61 gcgcgcgcgc ttcctggcgc tgctgcccta taccgatcag cactgaggcc tgcagccatg
      121 gaactgatac tgctgcagaa agtcgccaac cttggcaaca tcggcgatcg cgtcaaggtg
      181 aagtccggtt acggccgcaa cttcctgttg cccaagggca aggcaacgct ggcaactgcg
      241 gccaacgtcg cgcgcttcga ggcgcggcgc gcggaactgg aaaagctggc gaacgatgaa
      301 ctcacgagcg cgcagcagcg cgccgcggcg ctgaaggaat tcaagctgac gatcacagcc
      361 aagtccggta ccgagggcaa gctgttcggc tccgtgggta ccgccgacat tgccgaggca
      421 gtcaccgccg cgggccatac gctcgcgcgc agcgaggtgc gcctcccggg aggtccgcta
      481 cgtgccctcg gcgaacactc ggtacacatt cacctgcacg ccgatgtcga tctggagctt
      541 ccagtcatca tcgtcgccga agaagcgggc tgacgagggg gccgtcgcgt gagcactccg
      601 ctgcgctcac gtcgcgacgg cggcgccacg ggagccgaag ggctggttcc tccgcagtcg
      661 gtcgaggccg agcagtccgt gctcggcgca ctgctgatcg atacccacgc ctgggatcag
      721 gtcgcggaca tcgtcaccgc cgcggatttc taccgtcctg atcaccggat gatcttcgag
      781 gcgctgggcg agctggtcgc ggccggccgg ccgggcgacg tggtgacggt ttccgagcag
      841 ctcgagagac acggtcgtct gctcgacgcc ggcggtctgg cctatctggg cacactggcg
      901 cgcgataccc ccactgcagc caatgcccgc agctatgcgc agatcgtgcg cgagcgggcg
      961 ctgctgcgac gtctgctgga agcggggcgc gagatcgccg cgtcggtctt cgacgaggaa
     1021 ggcctgtcgg cacgcgagct ggtggacaat gccgagaagc gcgtgtttga aattgcggag
     1081 atgggctcgc gcggccgcga cggcgccgtg cgcgtcagtg cattgctgcc gccgctgatc
     1141 gacaagatcg atgagtggca taccaatcca aacaagctac gcggactggc gaccggattc
     1201 accgattttg ataggaagac cggcggtctg cgcggtggcg acctgatcat catcgccggc
     1261 cgaccttcga tgggcaaatc cacgcttgcg gtcaacatgg ccgaatatgc ggcgatcaat
     1321 cccgagatcc gggccagggt cgccatcttc agcatggaaa tgccctccga gcagattgtc
     1381 acgcgcatgc tggcctcgat cggtcacgtg ccgctcaaca gcatgcgcac cggacagatc
     1441 tccgatgacg attgggtacg cattacgtcc gcaaccagtc agctatccga agcacgcatc
     1501 ttcatcgacg agaccccggc gctgacgccg accgagctgc gcgcgcgttc gcggcgcatc
     1561 gcgcgcgagc aggggggact ggatctgatc gtcatcgatt acctgcagct catgcaggtt
     1621 cagggcaaca cggagaatcg cgcgactgaa attgccgaga tctcacgcgg cctgaaagcc
     1681 ctggcgaagg aactggacgt gccggtcatc gcgctgtcgc agctcaatcg cggcgtcgaa
     1741 cagcggcagg agaagaagcc ggtgatgtcg gatctgcgtg aatgcgtcac cgcagacacg
     1801 ctggtatgtc tgaccgatgg acggcgtgtt ccgatccagg agctggtcgg taccgctccg
     1861 gaagtctggt cattgaatgc ctcccaacga cttcacgcgg cccgcgccga caaggtctgg
     1921 cacgtgggcc gcaagagcgt gtttcgcgtg gagcttgcaa gtggccggaa cattcgggcg
     1981 accgcggagc accggttgct tgccggcgcc ggctggatga cagtgggaga gctgaagctc
     2041 ggcgagcggc ttgcggtcgc gcgccgtatt ccgccagcag caaagctcgc gagccggcct
     2101 gacaattggc tgatggcggg tgtgggcgcg tggctcaagc gcgtcagcgc gccaatgcag
     2161 ggacagcgta tctcgcgaac tcagatggcg aatatacgac gccgagccgt attggatacg
     2221 cagtccgcgc catcgggcgc gccggtttcg gaatacgcgc acattcttgg tgacgagaag
     2281 ccgcacgatc ttgcccgcaa cgacattttc tgggatcgcg tcactgcgat catcgccgag
     2341 ggggacgagg aagtttacga ccttaccgtg ccaggtccgg ccaattggct cgcggatggc
     2401 gtgattactc ataacagcgg agcgatcgaa caagatgcag atatgatact tctgatatat
     2461 agggacgaag tctacgataa gaatacgact aagaagggca tggccgagat cgacctcgcc
     2521 aagcaccgca acggcgagat tggcccattt ctgctgacct ttcagggtca gtacacccgc
     2581 ttcgtcaact acgcgcccga cacgtacgcc gagggcgtat tgcggtgatg cggctgattc
     2641 gcgcgcgcat cgacagcgcc gcgttgcagg ccaatctcaa gacgatacga agccacgcgc
     2701 ccaacagccg tgtcatggcg gtggtcaagg ccaatgccta cggccacggc ctggtaccca
     2761 gcgccttggg gctgatcgat gctgacgcat tcgctgtggc gcggctggag gaggcgatcg
     2821 cgctgcgtac cgccggcctc aagcaccgca tcatgctgct cgaaggcgtg ttcgatgccg
     2881 ctcagctggc ggaagccgcg cgacatcacc tggatctggt gattcatcag cccctacaaa
     2941 tcgagctgct ggaatcgcat cgcggctcac agcgctggtg tttgtggctg aaagtcgata
     3001 ccgggatgaa ccgcctcggc ttccgacccg agcaatttca cgacgcctgg cggcggctgc
     3061 aggcgttgca cacggcaccg atggaactgc gcgtgctgac gcatctggcc tgcgccgatg
     3121 aggcggatga gtccatcacc gcacgacagc tcgcgcgcct cgcgccgctg atcgcgggcc
     3181 tcgatgtgga gctcagtatt gccaactccg ctggcattct ggcgcagccc tcgacgcaca
     3241 gtgcctgggt gcgaccggga ctggcgctgt acggaatctc acctttcccc tccaggctcg
     3301 gctcgcagct gggactgaag gccgtgatga cactcgagag ccgcgtgatt gccttgcgcg
     3361 atgtcctgcc cggagaacag gtgggctatg gcggagtatg gacggcacgg cgacgcacgc
     3421 gcgttgcgat tgttgccgca ggctacggcg atggcctgcc gcgcagcctc cccaacggtg
     3481 ctccggttct catcggaggg catcgcggct cactggtggg gcgcgtctcc atggacatgg
     3541 tggccgtcga tgtgacggat ctgccgccga tacccatcgg tgagcgcgtg gaactatggg
     3601 gaccgcatct ggcggtcgag gaactggcac gggccgcagg taccatcccg tatgagttag
     3661 tgtgcggtgt gagtcagcgc gtaccgatcg aactggtcta gagttcgtcc gtggaagccc
     3721 tggtgcaatt cggtacgcgc ctgtacgacc gcgtcgagcg gctggtgcgt gctgggctca
     3781 ttgccgcccg gcaccgggag ctcagtggca tcgtcttctg ggccgcgctg atcggcattt
     3841 gtggtgcctt caccggggta gcctttcggg ccagcgtgcg cctggtgcag cgtctgttga
     3901 ccggttcttc cgcaggcctg gtcgagaccg cggaactgct gccctggtgg gcgcgcatca
     3961 tggtgccgac catcggtggc atttgtgccg ggacgctgct gtgggcggga caacgcatcc
     4021 tgcggcagtc gcgtaccgtt gactatatgg aagcggtggc cgtgggcaac ggccagctca
     4081 gcagtcgcgc ctcgttgctg caggccacgt cctcgttctt cacgatcgga tcgggagggt
     4141 cgatcggtcg cgagggaccg ttggttcaac tggcagcgac cgtcgcatcg aagatcggtc
     4201 agtggactcg agcgccggtc ccacgcctgc gtctgctggt ggcttgcggc gcctcggccg
     4261 gcatcgccgc ggcgtacaac gctccgatcg ccggtgcact gttcgtggcg gaagtggtgc
     4321 tcggctcgat cgccatggag agcttcggcc ctctgatcgt ggcctcagtg gtctcggatg
     4381 cgacaacgcg acacttcctt ggatacgggc cggtctattt ggtcccgcac atcaattttg
     4441 gcgcgacctg ggagctggcg ctgtatgcgc tgctcgggat ttttctggga cacgcagcac
     4501 cgccgtatct ggcgctgctc gataacgcgc gccggcgctt tcgacgactg cgctggccac
     4561 tgccggtcac gctggcgctc ggcggcttgg tggttggagc gatctcggtg ggcgtgccgc
     4621 aggtctgggg caacggttat agcgtcgtga acgacatgct caacgacaag ctgtcgctgc
     4681 aactgctcgg actggtcgtg gtggccaagc tcatcagcac cacggcatcg gtgggttcgg
     4741 gcgcgaacgg gggaatcctc acgcccacgt tgttcatggg tgcggcgttt ggagccttgt
     4801 acgcgcacgg actgcacttc gcccatccgc agctcagctc gcagatcgcg gcctatgcgg
     4861 tagtcggcat gggcagcttt ctggccgcga ccacgcacgc acccctgacc tcaatcctgc
     4921 tgatcttcga aatgacgctg gactatgaag tcgtcctacc gttgatgctc gcgtgcgtta
     4981 ccggtcacta cgtggcgcga atctaccggc agggtgaatc ggtctatacg cgtgcgctga
     5041 aagccaaagg tccgggggaa aaggaagagt ggaacctgcg gcagatcacc gcactgattc
     5101 gtcccgcgag tgtgcaggtg caccgtgact cactgctggg caatgccatc gatcgaccgc
     5161 caggcagcgc caacagtgct tatgtggtgg attcgcagaa ccagctggtc ggccagctgg
     5221 acatcgcggg cgtgaagcgt cagctgcgca ccacgccggc aggcccgctg gcgttggtgg
     5281 ctacctatat gcagccggca ccaccggtac tcacacccga gatgctgttg ggagatgcgc
     5341 tggatgtctt cctggcctat cgggcgaaac aactaccggt ggtctccggg cagtggagtc
     5401 cgattctgat cggcgaagtg tcgcgccacg acctgctgct ggcgctgcag gaccgtatgg
     5461 cggaacgacc caatcaggtg ccggcgcatt agcgccccga cgcactagcc ttcgaagaac
     5521 cccatcttca cgccggccag ctgaatcaca tcgttgtcgg cgagcttgcg ggcctgagcc
     5581 ccgatcggcg caccgttgac cagcgggaag ttgtcggcct gttcggcatc cacgtgcacg
     5641 atgaagaacc cttcggaacg acgcgtgatg gctgcaacct gcacgccagg gcgtccgagc
     5701 gtcgtcagag ctttggtcag ctccagttcg cgtccggcaa aggcaccgga gagcacctga
     5761 agctttgctt tcctgagtgc gggagcgcca ttggcgatcg cgcgccgcgc gccggtcgcc
     5821 gaggtatccg tggtggccgc gcgcaccttg tctaccggtc gcgccgacgg ctgaatcacc
     5881 atcgtctttt caaaatcgtc ctgtgcagcc tcgtcctctt cgacgaaacg tagctgatga
     5941 tggccggcgg tgatgacgtc cccgtgctgc aaagcatgct tcttgatgag cttgccgttg
     6001 acgtaagtgc cattggtgct gttcagatcc tcaaggaacg aatcgttgag gatgttgatg
     6061 atcagcgagt gatggccgct gactgcgggg ttatcgatgc gaatatcgtt gtcaggaagg
     6121 cgcccgatcg tgtagcgctc cttgttcatg ttgtattcgg cgaggaccgc accgtcgaga
     6181 ctcagaatca acctagccat gcatttagcc tcaggtagag ctgaaccagc ccaacatctt
     6241 gtcaaagata ccgcgtggtg cggggaaaga ttccaggatc tccgctacca cgaccgagat
     6301 gttgtccctt ccaccgttat cgttgcccaa ctggatcagc tgtttagtgg cggtctcaag
     6361 gttagcacca aaggtgctga tggttaagtg gatatcgtcg tcctcgacca tgtccgacaa
     6421 gccatccgaa caaataagat acatgtcgcc cttctgaacc gggtgctcct gcacctctac
     6481 ttcgacgttt tgctccaccc ctaaggcgcg ggtcacgtaa ttcttgttgg ctgcgcgctg
     6541 cgcctcctcg ggtgagtaga aaccgcgatc caccagttcc tgcaataggg agtggtccat
     6601 cgtgatctgc tccaggtcgg cctggcgcaa tcggtacatg cgcgagtcgc ccacgtgggc
     6661 gatcgagatc cggttgtcga agaacagggc gccgaccacg gtcgtgccca tcccttcaca
     6721 ttgcggttgc gagcgagcgg tctggaagat gatcttgttg gcccggacaa ttgcatcgcg
     6781 caggatgatg gatgcgcgag tcaggcccgc ttccgaatcg tgtacgcgca gatcctcgcg
     6841 ttcgaccgat tcacgcacca gtccaacgat agtcttgacc gcgatgccgc tggccacttc
     6901 gcccgcgttg tagccgccca tgccgtcggc cagaaccatc aggccgatat cagggtcgta
     6961 ggcaatggtg tcctcattgt gatcgcggac ccgaccggta tcggtctgcc ctacgacctg
     7021 aactttcccc ttgaggctca tccggcctcg tcctcgcggc atggttgttc gcttcgtcaa
     7081 aacttgaact gtgtcacagt cgtcgttcgt gatgtctcat accgcacctg tcctgggtcg
     7141 gagcggcaag cgcgtaactt agcatcgtgg ctcatccaat gcgcgcctct gatcacagtt
     7201 gcggtggaac cgggcggggg gtgtagcatc cgcgccttgg cacgaactca aacactctat
     7261 gtctgccgct cctgcggtgg tgaggccgtc aaatggcaag gtcagtgtac ccattgtaaa
     7321 gaatgggact cactcgaggc agtgaccgca gttcgcgggg tccgacagat gaccgcttcc
     7381 gaggctgttg ccggcccgct gcaggatgcg accgagaata ctgcctccgc ggtcaggcac
     7441 tccttcggct tcagtgagct cgaccgcgta ttcggcggcg ggctggtatc agggtcggtc
     7501 acactgctcg gcggcgagcc gggcatcggt aagtccaccc tgttattgca ggtcgccgca
     7561 gcgctttcca atcagcaaaa gaccctctac gccagcggcg aggaatcgac ggcgcagatc
     7621 tcgctgcggg cccggcgtct gcagctcaac gccccgcagc tggcattgct cagcgagcat
     7681 tcgctggatg ccatccttgg gttggccgct gcgcgcaagc cagacctgct gatcgttgac
     7741 tccattcaga ccatgcagct cgcggcgaac gaggccagcg ccggctcgcc ggcgcagctg
     7801 cgcgagtgcg cggccttgtt ggttcgcttt gccaaggcga acgcctgcgc cgtgatcatc
     7861 gtcggacacg tcaccaagga tggcagcatc gccggtccga agctgttgga gcatctggtg
     7921 gacacggtgc tctattttga gagtgatgcc ggcagccgct atcggatgct gcgggccaca
     7981 aaaaatcgct tcggtccggt caatgagctc ggtttttttg ccatgagcga cgcaggactg
     8041 aaagaggtca gaaacccttc cgcgatcttc ctatcacgtc atcccgagcc ggtgcccggc
     8101 agtgtcgtga tggttgcgcg tgacggtggc cggcccatgc tcgtcgaagt acaggcgctg
     8161 gtcagccgcg ccggcttcgg agctccgcgg cgcgtggcgc agggggtcga tagcaaccgc
     8221 gtgtcgatgc tgctggcgat cttgagtcgg catgccgatc tggcattggc cgactacgat
     8281 gtgtttgtca atctggttgg cggtctcgac attgccgaaa ccgccaccga cttgccgctc
     8341 gcgctggcgc tggcatcgag cctgaaagat cagccggtgc gcgaatcgct ggcggccttt
     8401 ggcgaacttg ggctgaccgg cgaagtgcgt cccgtcgcct acggcgatga gcgggtgcgg
     8461 gaggcggtca aacttgggtt cagccgcgtg atgctgccgc gggagaatct gccgcgcaca
     8521 ccgcccgagg cagtctcgct acagccggtg tcgcgtatcg ctgatgcgct gagcctcgcg
     8581 tttgcctgaa ggactcaccg gtccttggac gcggcccgga ccctgaccgt gcggatcgcg
     8641 ttgtccgcga tctgcagcac ctcgaagtcg tagtcaccga tgcgaagcgc tgtgcccggc
     8701 ggcgggatgg tttcgagttt ctccagcagc aagccgttca gcgtgcgtgg gccgatgtcg
     8761 ggcaactgcc atccgagcga tcgattgagc gcgcggatcg tggccgacgc gttgaccaac
     8821 cagttgccgg cggcatcgcg gctgacgtcc ttgtgggtca cggttgccgg atcgttggtg
     8881 aattcgccga cgatctcctc caggatgtcc tcgagcgtga ccagtcccgc cacatcgccg
     8941 tactcgttga ccacgaaggc gaggcggcgg cgagtacgct gaaactgcgc cagctgttgg
     9001 gtcagcgtgg tgccttccgg cacgaaatag ggttcacgcg cggccgcgat ctccagcatg
     9061 cgctcgcgag tcagtgagcc gcgcgccagc tcctgcgcga cgcgcttcat gtgcagcgtg
     9121 ccgacgaggc tgtccagttc gcggtgatag accggcaggc gcgaatgtgg ggtctggcgc
     9181 aaacgatcca gaatgtcttc ccagctcaca tccgcgtcga tacctgcgat ttcctggcgc
     9241 ggaatcatga tgtcattcac tgtggcgcgc tcgaggtcga ggatcgacag cagcatctgg
     9301 cggtgtcgtg cggggatcag tgcgccggcc tcgttcacca ctgtgcgcag ttcatcggct
     9361 gacagcccgt tgccgccagc actggagcgc gtcacaccaa acagtcgcag aaaaccctgc
     9421 gcaacgtagt tcgcgagcca ggtcaccggc caggtgatcc acagcagcag ccgatagaca
     9481 taggcgcagc gcagcgcgac gccttctgcg tggaccgcag cgaagatctt cggcgccagc
     9541 tcgcagaaca cgatcacgac aacggtcagc aataccagct cgaacacgat cgcatgcggc
     9601 agtgcgctgc gcagtgccag cagggtcgcc acggtcgatg cggcgacact ggccagcgcc
     9661 aggatcacca gattcgcccc cagccagcga tccggttgcg ccagcagctc ttcgagcagt
     9721 cgtgcgcgcc gctcgccgag ctcagcgcga tgacggatgc ggtagcggtt caccgacagc
     9781 atggccactt cggtgcccga ggagaaagcg atcaccagca gcaggccgaa caggatgagg
     9841 atcaggacga tcgtcggcga tttatccacg cgtctcttat aatcggtagc ccgaaagctg
     9901 tataggccgc cgcatggcgc ggcgccatgg tggcaaatcc ttggaagcgc tgttgggtcg
     9961 tgcgctcaga tgcggggtgt catgtttgac aagttgacgg aacgcctggg tcgcgcggta
    10021 cagacgctgc gtggtcgcgg tcgcctcacc gaagagaaca tcgccgagac gctgcgcgag
    10081 gtacgcatgg cgctgctcga ggcggacgtc gcgctgccgg tcgtcaagac tttcatcgac
    10141 agcgtgaagg ctgaggcgct cggtgcggag gtggccgcta gcctgacgcc aggtcaggcc
    10201 ttcattggca tcctgcaccg ccagatggtg gcattgctcg ggcaatcgca agcgggtttc
    10261 gcccttaatg tgcaaccgcc tcaggtggtg ctgctggcag gtcttcaggg tgccggcaag
    10321 accacgaccg ccgccaaact ggcccgctgg ctgcgggagt cgcaccgccg acgcgtgctg
    10381 ctggtgagta ccgacgtgcg gcgaccggcc gccattttgc aattgcagcg tctgtcggag
    10441 agcgtcggcg ccgatttctt tccgagcgac tcgagccagg caccggccgc gattgctttg
    10501 gcggcgctcg acgccgcgcg ccgtggcgtc tacgaaacac tgatcatcga caccgcgggg
    10561 cgattgcacg tggatgccga actgatggag gaagtgcggc agatcgatcg cgccgtctca
    10621 tcgcatcagc ggctgtttgt cgtggattcg atggccggtc aggacgccct caatgcggcg
    10681 cgcgctttca gcagcgcact ggagctgacc ggcatcgtgc tcaccaaagc cgatggcgat
    10741 gcgcggggtg gcgcggcgct gtcggtgcgc catgtcaccg gcaagccgat cgtgttctta
    10801 ggcgtgggcg agaaagtcga tgccctgcag ccgtttgatc ccgagcgcat ggccacccgc
    10861 attctgggga tgggcgacgt ggtctcactg gtggaagagg ttacccgcaa ggttgacgtt
    10921 gacgaggcgc agaagctcgc ccgcaaggtg gtctcgggca aggcctttac gctggtggat
    10981 ctgcgttccc agctcgaaca ggtgcgcaaa ctcggcggcc tgtcggcggt catggacaaa
    11041 ttgccgagcc agttggcagg caaggcggcc gtcaagggtg atcaggccga tcgcgagctc
    11101 aagcgccaga tcgcgatcat cgattcgatg acccgccagg aaagacgtca gtcggggatc
    11161 attgacggct cccggcggcg acggattgcg tccggggccg gtgtccaggt ccaggacgtg
    11221 aatcgcctgc tcaagcaatt catggacatg cagcgtctga tgaagaacat gaagggcgga
    11281 aagctggcgc gcatgatggc gggtctgaag ggcggcattc ccccggggat gccgcctcgc
    11341 cgctaacagc gatcgcctcg gtataagcct ttattctggc ggcggatttg cgtagaatcc
    11401 gcgctctttt ttggacccta ccaaagttcg attatggtca ctattcgtct gactcgccgc
    11461 ggcggcacta accagccttt ttatcatgtg gtcgttaccg acagccggga acgccagggc
    11521 ggttcgagcc ttgagctggt gggttatttc aaccctgtgg cccgtggcaa ggaaacgcgt
    11581 ctgaaactgg atttggaccg tatcgatcac tggatgaagc atggcgcgca gacctcggag
    11641 cgtgtgcgtc acctggtcgc cggttaccgc aaggaagccg cgaagcaggt agccgccgcc
    11701 tgagccgcta tcgggtattg ggccggataa tcgggccgca cggtgttcgc ggcgctctga
    11761 aggtcctgtc gtacgcgcat ccgccgcaat cgctgttgca gtaccgcagt tggcgcttgc
    11821 gtcgcgcaga tggcggcgag gatgagtacc gggtgctgca gtcccagtgg gacgggcacc
    11881 tgatgcgcgc gcagctggaa ggtgtcgccg atcgggatgc cgcgcagcgg ctgcgtgagc
    11941 gcgagattct gatcgagcgc gatctgatgc cggcggcggg tccgggtgag tattaccgcg
    12001 aagacctgca gggcttcacg gtacgcaatc tggagggggt cgtgctggga acgctgcaat
    12061 acttcctcga ggcgcccgcc ggtgcgttga tggtcgtgag cggcgagagg gagcgctggt
    12121 tgccgacgac ggcaccgttc ctcaagcgcg tggacctgga gcggcgcgaa atagagctgg
    12181 actggccagc ggacttctag cgatgcacat cgaagtggta acgctatttc cggggctgat
    12241 ccaagaggcg ctgcgctacg gtgtgctggg acgcgcgatc gagcgcgggc tgctgcaagt
    12301 ggaaacggtc gatccgcgca gcttcacgca cgatgcgcat cgctcggtcg acgatcggcc
    12361 gtatgggggt ggaccgggca tggtgctgac gcccgaaccg ctgtgcgcgg cgatcgctgc
    12421 cgcggccgag cgctcgccgc ccggaagcgt gcgagtcgcg ctgtcggcgc agggagagct
    12481 gctgaaccag gcacgggtgg cgcagtggtc gcagcggccg ggtctgatac tgatcgcggg
    12541 ccgctatgag ggaatcgacg agcgggtgct gcagctgtcg gtcgaccagg agatatcgat
    12601 cggcgattat gtggtttcgg gcggggagat gccggcgctg gtactgatcg atgcgctggc
    12661 gcgattgctg ccgggtgcgc ttggatgtgc gcgctcgaat gtggaagagt cgttcgacac
    12721 gggattgctg gactggccgc actacacgcg gcccgaaatt tttcgggggc aacgggtgcc
    12781 ggaggtactg agctccgggg atcacgccgc gattcgtcgc tggcgtatga aacaaagtat
    12841 cggccgcacc tggctgcgaa ggccggaact gatcggcaag acgccgctcg acaccgagcg
    12901 cagggtcttg ttggaagagt ttttgatgga gcagctcgcg aaggcggctg acaactgagg
    12961 agacaacgat gagcaacatc attcagacat tggaagccga gcgcatgacc cgcaaagtgc
    13021 cggcgttcaa gcccggcgat acggtcgtgg tgcagatcaa ggtggtcgaa ggcgaccgcg
    13081 agcgggtgca ggcctatgaa gggatcgtga tcgcgcgcag caatcggggc ttcaattcct
    13141 cgttcacagt gcgcaaggtg tcacacggtg aaggcgtgga acgcaccttt ccgattcata
    13201 gtccgagcat cagcgaaatt tccgtcaagc gtcggggcag tgtgcggcgc gccaaactgt
    13261 attacctgcg cgatcgcgcc ggcaaggccg cgcgcatcga agagaaggtt tagcgccacc
    13321 ctgtgcacgc tctcggtcgc atcatctacg gcgtctggcg cggcctggat gtgctgcgtc
    13381 gtttcctgca cctgctggtg ctgctggcgc tgttcgggat cctagtcggc gcgctgcgcc
    13441 aggtgacgcc gcgtgtgccg gagaaagccg cattggtggt gcgcccctcg ggcgatctgg
    13501 tggagcagat cagcggcgag ccgctggcgc gcgcactgag tgaagcgcag ggtggcggca
    13561 tgccgcagac c
//
LOCUS       DATMRM010000491        15119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3377103, whole genome shotgun sequence.
ACCESSION   DATMRM010000491 DATMRM010000000
VERSION     DATMRM010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15119
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3377103"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..4165
                     /locus_tag="VNZ06_14930"
     CDS             83..4165
                     /locus_tag="VNZ06_14930"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWW22092.1"
                     /translation="MRLRSVRLQRNPAEHVRYFQYAAVPRPFPQSHGACHTSLPRLNE
                     ALLRCFRHPDLRRTHRLFVEEVDPLLDALCDRFVAESMRGTDAASLPQGPYQSHLLDT
                     AQADAAYEAANAETSSAQDIWNSLLADYPGFFPLVHAVGCVGMQLPALLQEQTTLSEV
                     LPDRLTVAALQRQVLGDIAQRQIIAVLRAEIEQALVDLEEGQRLTIIELSASAAPLAT
                     ELCRGLDFDRADYHFVSTQSTALEEGRRLQDRYPAITVESLGDQPTGGRDCSLAIVTL
                     DFHSLSDSQRALDFAQSRLVRGGVLLVLGQYPSHTLDFVYGADRAWWSDSPQGEWHSP
                     QQRPEFWVHYLQQIGFGQLRQHEYGSDPPCGCYTLLAQCDTGNATEQIPVVAAVGRHW
                     LLLADSDGISSQLAEHVASRLSRIGDRASIATPLDASALARTLSEANIGDSALFGIVH
                     LSGLCALESESPAAILEVQLQRCSIAASLIQACESTETDATCCFVTANALRHLLPDRS
                     PGDADNQSDIIDASLAGFARTLMNEAPIGAVRLIDLEINESSIESTAAILVSELCIDD
                     AEQEIILTTSGARYAPRLRACEAPHGDPAATPGTETRRALGFDVPGQLRNLQWNRIPL
                     AALGDEEIEVSVRATGLNFRDVMYALGLLSDEGVEHGFAGASLGLEFSGVVARLGARV
                     MGFAVGDAVVGFAPSSFGDRLVTKAGALWHLPAGLSFEAAATIPSTFFTAYYSLRTLA
                     GLREGEKVLIHGAAGGVGIAAIQVAKWCGAEVFATAGSDEKRNFVRLLGADHVFDSRN
                     LAFAEQVLRCTDGRGVDVVLNSLAGEAINRNFRVLKPFGRFLELGKRDFYENTRIGLR
                     PFRNNISYFGIDADQLMNEKPELTRQVFAEVLQLFNERVFHALPYQSFEARDVIDAFR
                     YMQQARQIGKLVVTYGSGIPDPACTSEGPVQRLELSPSATYLITGGISGFGLRTAEWL
                     ADRGARSLVLLGRRGAGADEAQTVIARLERHGVHVAAWACDVTDRLALHDALERIARE
                     LPPLRGVVHAATVIDDALIREMSEAQLRRVMSPKVLGALHLHELTRSLPLDFFVLYSS
                     ATTLFGNPGQSNYIAANAVLEALGRARRAAGLPATCVRWGAIDDVGFLARSPKLKQAL
                     QDRMGGSALQSAVALDVLEAVLLSGRSDLGVLELDWRVMRRSLPSAAASKFVEIARGI
                     LGEVEDDASLDLDRMLRELPAEQLQDAVTDLLRAEVGEILRIAPDKIDALRPMQEMGF
                     DSLMGVELVVAVENRFGVRLPVLVLSDSPTVSKLAAWIIKQLRSEEGAASVAQADVTR
                     AQIERIASQHAVNTPSAAEIERIATELRSDESGATRRMIH"
     gene            4184..5530
                     /locus_tag="VNZ06_14935"
     CDS             4184..5530
                     /locus_tag="VNZ06_14935"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWW22093.1"
                     /translation="MNRKTLPGLTANLKEKLIQQSLERRLNQASASAAQVRTLHQNGA
                     SNDIPEDFYRFDRHPGYQQLRIIADGAARMEIGSPFFKVHEGIAGARTRIGGREYLNF
                     SSYNYLGLSGHPAVLDAAKQAIDRYGTSVSASRPVSGERALHRELESELARAYAVDAA
                     VAFVSGHATNVTTIGYLFGPRDLIVHDEFIHNSSLQGIQLSGARRLSFPHNDWAALDA
                     LLKEHRRKFERALVVIEGIYSMDGDFPDLPRFVDIKQRHNCFLMIDEAHSFGVMGERG
                     LGIREHFSLPGDAVDIWMGTLSKTLAGCGGYIAGETALIEHLKFLAPGFLYSVGMAPP
                     VAAASLVALRCLRAEPARVATLHERGAQFLALAQAAGIDTGSSAGIAVIPAIIGSSTR
                     AARISAELMHRGINVQPILYPAVPEKLARLRFFICCEHTEADIREAVGALATEVRR"
     gene            5543..6337
                     /locus_tag="VNZ06_14940"
     CDS             5543..6337
                     /locus_tag="VNZ06_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22094.1"
                     /translation="MNRVPPNPAVLCQLYLDAVLPCLSELVAEDAFARDLTAGLNASI
                     VFRVAGGPARSVHLRDGAVSSQPGSCSHPSIVLLFLSRAHLNAFFSGRKWALPLLAWG
                     GWRIRVLKGFSTLAERLEAVLEGHEAVISTAAGRRLHARLSLMAAGLGLPSLAAGDVV
                     SREAFRSLPYGMASFTISSEQGATVWFDRQLDTSRAGWSAPPRQAEVCIAFGDAETAF
                     AAMRDQIDTMAATASGQIVVDGLIPLADGLNFIMQRLRVYLQPSKS"
     gene            6334..7575
                     /locus_tag="VNZ06_14945"
     CDS             6334..7575
                     /locus_tag="VNZ06_14945"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWW22095.1"
                     /translation="MTATKDRYHRSLELFERASQVIPCGIYGHTAPAAALPGAFPYYA
                     QRAAGSRYWDVDGNEYIDYMCGYGPVVLGHQHPEIEEAAVRAQRDTLCVNHPTPIMVE
                     LAESLVSLVDFSAWAVLGKNGSDMTSWAIQVAREHTKRRKILKVSGAYHGMDPWCTPG
                     HAGLIEEDRVHIHDFNWNDLDSFDRLLKRYRDDIAGVIMTPFHHPVFNASQMPSDGFW
                     PHIEAACRRDGIVLILDDIRAGFRLDLRGSHKLFGFEPDLICFCKAIANGHPLAATLG
                     RKEMRVAASKVFLTGSYWNSAPAMAAAMKCLAILRAQDVPSQLHRTGESLMQGLVERG
                     RRHGYTVQPSGPAAVPFITFDSDPSMRRRQRFCAEVTRRGAFFHPHHNWFICAAHSSE
                     DIKTTLAMADEAFAVLRAENL"
     gene            7591..8442
                     /locus_tag="VNZ06_14950"
     CDS             7591..8442
                     /locus_tag="VNZ06_14950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HWW22096.1"
                     /translation="MAIVALKNHPRLSIEVHATPDKFISSQIVETGEWEPFETHLVEC
                     FLRPGDVFVDIGANIGWYTIIAASIVGNNGYVYAFEPGTANFELAARNLVLNDLQNVT
                     LEQIAIADRTGTGRLFLSRENLGDHRLFAAEEERCSQTVSVTSLGRYFEHNPTPIRML
                     KIDAQGSEARIFEGLPDDFARTRKVAAILLEFWPYALEQSGSSAKSLVRSLAAQGMSC
                     YIIHECFRGLDPIDLDVLEQRAYGDLRENNLFLNLLALPATNAVPAAIRTMIRPPDAF
                     LFYHAAR"
     gene            complement(8463..9281)
                     /locus_tag="VNZ06_14955"
     CDS             complement(8463..9281)
                     /locus_tag="VNZ06_14955"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWW22097.1"
                     /translation="MKRIFLTGVSSGIGLCVAKALCQRGHQVWGTSRSRERLPDLSGF
                     RGVVLDLNDDGSIDAGFREALAAAGEFDVLINNAGAGMFGPIEGFTCDELRSQFQTLL
                     VGPLHLIALALPAMRASDRGLIINISSLAGELPVPFLAPYSAGKSALSTLSDGLNLEL
                     AHTGIRVVDVRPGDFATAFHPSTRRVGDRLSDAYAPNLEIAWRAIDRNMARAPDPQLL
                     ANAVVRIIDGEIRRPAVAVGDLFQARIAIALARRSLRAWVHWGLRKYYGLRHGR"
     gene            complement(9288..10868)
                     /locus_tag="VNZ06_14960"
     CDS             complement(9288..10868)
                     /locus_tag="VNZ06_14960"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWW22098.1"
                     /translation="MSAELDELKDIGFSALVGLKNDTLAGWYNQEKGELTDGFPVSSE
                     DVVLDVGCGIGGMATFCAQRGSQIILIDSNPANLGQARARLARYHGLRLETHVSDAHR
                     LPLTSESVSRVICTEVLEHVDDPTAVMAELFRVGRPGALYLITVPGTAQEMLQKRLAH
                     PSYFEKPNHVRIFEPQSFAALVQGSGLTIEHTMSQGFFWSIWMALFWQANVPLSNASS
                     HPSLDFWARTWAEVLKSEQARHVQTVLDEFLPKSQVIVARKPHQVTRTDGVPSFFEAT
                     QHLRDGDKCDEQVRDRLTVEIGRRLECAQVKSHPRLPQVTDLRMRREGLPDWWTAGNN
                     VLLASPKSKTPTLRAGFFELPLPRNVILVVGEEAAINHVNVAGEGGLVVLGDKVQIQS
                     GAMSAIGYCTILIGERTGATMWAEVDARNGGVVSVGADGMWAIGVRLYTDDSHAIRDV
                     ATGKRINKMGGVIILESHVWLCRDAEVMGDCHVGADSVVGMGSLVKDAELPANTVSVG
                     RPARVVRTGTTWSRLDLP"
     gene            complement(10865..12208)
                     /locus_tag="VNZ06_14965"
     CDS             complement(10865..12208)
                     /locus_tag="VNZ06_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW22099.1"
                     /translation="MIVLEHSTFDARAIGGRLGAPDYSYWFVRKAFRPVFERIGIIVP
                     ITDPAREVDRIAKNAASYGESSVYFSFSPPHHTMVASVCPTVPVFAWEFDTLPDEPWQ
                     NEPRNDWTTVLSQVPAAITHSRFAVQVVRRYLGENYPIWSIPAPMYQSNVRRAVSAKG
                     WQSPTTLTISGFAIDAGAVDLSLFNVDRSRTDGSKAVKALNARVQSRGNKPLRFSLSG
                     VVYTAVFNPVDQRKNWTDLLAAFIWAFRDNPEASLLIKVTHYDAVVGVVPILSDLSKH
                     CDFKCRVLIVHGMIAQEEYDSLVDVTSYAVNVSTGEGQCLPLMEFMSAGRPAVTPLHT
                     AMLDYVSSDNSFVVGSTRKPTSWPHDPRQSISCTQYAINFSQLVQAYRDSFDVAKNQS
                     ERYTAMSAAASAAQQRFCSDEVVSSRLREVFEHLGLRPRTNRATFMRRVLSRVAT"
     gene            complement(12205..13935)
                     /locus_tag="VNZ06_14970"
     CDS             complement(12205..13935)
                     /locus_tag="VNZ06_14970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWW22100.1"
                     /translation="MAGEEDSEVQGYVDGVREGRICGWAWRPSTPHETLTVEVLIDGV
                     VAGEAAAWLHRPDLAVAGVGDGRHGFAVPVLLDMDRLGLSRIVVRVKDGDPLPGSKLL
                     VDAHGDVMDEVTEPDIAAFIESVLNQTAAKRSAVSAEPAAEAQSPPFCARQGVANNFI
                     VYSSTNEKSLARDFGGPEYSYYFVARAFNPLLARLGTVHVASDPVDEVNRIYEQCAAR
                     GENCLFVYYAPPHRMRLGSRCPTMCVIAWEYSNIPTENWGDEPRNDWRRVLRHTAGAI
                     TLSEYAAVAVRQAMGDQYPVLAVPAPVWDRSQTAGSKLALRAPASSAELALDGFVFDS
                     RQGSFRMGEAPPAPPRPTPKGPAKSVLASIDGVVFTSVFAPKDGRKNWQDMLTAFLSA
                     FIDTPDATLVFKMIGPESYWWWETHDLLARMPPARCRVVVLQGFLPDAHYARLVGASH
                     WVLNSSVAEGLCLPLLEFMCAGRPVVAPGHTAMADYLNAENSIIVRSDDDYCAWPQDR
                     RCHFNSRRYRIEWESLRDSMREAYRITKSDPSRYEAMCNAALRTMSTFCNDERVAERI
                     ASFLGLRVAA"
     gene            complement(13946..15058)
                     /locus_tag="VNZ06_14975"
     CDS             complement(13946..15058)
                     /locus_tag="VNZ06_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22101.1"
                     /translation="MFACLPAAAAEQQPATALGPVHARADTVLRPISRDGGFSVQYSK
                     QPARSLWLFGDTSQKNGPPFIGGTTAAIAAVVAGKAPTDMAEVPTPPTMPASPLPSPQ
                     PFFPTPVGLGAPGSAGRAMLPCGAHGSGSLYPAAWPTGGARIPRSSKVLLVYFQVCVT
                     SKAFPVERLSLTIYDPQHNQFLSSWTPFVAKPLSAGLARPEALESPVFGGDGYLYLYA
                     HEPLGGVYVARVSSEPSAWGNASSYAWWSAPPQSEGRWSSDETAASSLVSGIPVFGAS
                     VSDLGATTLHKFVMLIQTGFRTAEFALYEAPAPAGPWSAGPTGRVPDACAIGVFGCYA
                     LNLHPELSTREQVVYSWYSPGKAPDQGRINLGAVRW"
BASE COUNT         2898 a         4778 c         4542 g         2901 t
ORIGIN      
        1 gcttcgtcga gggccacatt ccctgtgcgc cgagtacgca ctgttcgacg cggagggtca
       61 accgatcgct gtgttggaag acgtgcgcct gcgcagcgtg cggctgcaac gcaaccccgc
      121 cgaacacgtg cgctacttcc agtatgccgc ggtccccagg ccgttcccgc aatcgcacgg
      181 cgcctgtcac acttcactgc cacggttgaa cgaagcgctg ctgcgctgtt ttcggcatcc
      241 ggacctgcgt cgcacgcatc gcctgttcgt cgaggaggtc gatccactgc tggatgcgct
      301 ctgcgatcgt ttcgtcgcgg agagtatgag gggaacggat gcggcgtcgt tgccgcaggg
      361 tccctatcag tcgcatctgc ttgacaccgc ccaggcggat gccgcctacg aagcggccaa
      421 cgcggaaacc agctcggcgc aggatatctg gaacagtctg ctcgcagact atcctggctt
      481 tttcccgctg gtacatgccg tgggctgcgt cggcatgcaa ttgcctgcgc tgctgcagga
      541 acaaacaacc ttgtcggagg ttctgcccga tcgactgaca gtcgccgccc tgcagcgcca
      601 ggtgctcggc gacattgccc aacggcagat cattgctgtc ctgcgcgccg agatcgagca
      661 ggcgttggtt gatctcgaag aaggccagcg actgacaatc atcgagctaa gcgccagcgc
      721 cgcccccctg gcaaccgaac tctgtcgtgg gctggacttc gatcgcgcgg actaccattt
      781 cgtcagcacc caatccacgg cgcttgagga aggtcggcgc ctgcaggacc gctatccagc
      841 gattaccgtc gagtcgctgg gcgatcaacc caccggcggc cgcgactgct cccttgccat
      901 cgtcacgttg gattttcatt cgttgagcga ttcgcagcgt gcgctcgact tcgcgcagag
      961 ccggctggta cgtggagggg tgttgctggt gctggggcag tatccctcgc atacacttga
     1021 cttcgtctac ggtgccgatc gcgcctggtg gagcgactca ccgcagggcg agtggcactc
     1081 tccgcaacag cgaccggagt tctgggtgca ctatctacag cagattgggt tcggtcagct
     1141 gcggcagcac gaatacggct cggacccgcc ctgtggttgc tatacgctgt tggcgcagtg
     1201 tgacaccggt aacgccaccg aacagatccc cgttgttgcc gctgtcggcc gacattggct
     1261 gctgctggcc gacagcgacg gcatctcctc gcagttggcc gaacacgtgg ccagtcgctt
     1321 atccaggatc ggggatcgcg ccagcattgc cacaccgctc gacgcttccg cgctggcgcg
     1381 cacgctatcg gaggccaaca ttggcgacag tgcactgttt ggcatcgtcc atctgagtgg
     1441 attgtgtgca ctggaaagcg agtcccctgc tgcgattctc gaagtgcagc tgcagcgctg
     1501 ctccatcgcg gcaagcctca tccaggcgtg cgagtccact gagaccgacg ccacctgttg
     1561 tttcgtcact gccaatgcct tgcgtcatct gctgccggac cgttcgccgg gcgacgccga
     1621 caaccagtcc gacatcatcg acgcatcgct ggccggtttt gctcgcacgc tgatgaacga
     1681 ggcaccgatt ggtgctgtcc gcctgatcga tctggagatc aacgaatcgt cgatcgagtc
     1741 gacagctgcc atcctggtga gcgaactctg cattgatgac gcggaacagg agatcattct
     1801 gaccaccagc ggcgcacgct acgcgccgcg actgcgcgcc tgtgaggccc cgcatggcga
     1861 tccggccgcg acaccgggaa ccgagacgcg tcgcgcgctc ggattcgacg ttccaggcca
     1921 gctgcgcaat ttacagtgga acaggatccc tctggccgcg ctcggcgatg aagagattga
     1981 ggtgtcggtc cgagccaccg gcttgaactt ccgcgatgtg atgtacgcgc tcggacttct
     2041 gtccgacgag ggagtcgaac acggcttcgc cggcgccagt ctgggactgg aattctctgg
     2101 ggtcgtagca aggctcgggg cgcgcgtcat gggatttgcc gttggtgatg cagtggttgg
     2161 ctttgcacca tcgagctttg gcgatcgcct cgtgaccaag gccggagcgc tctggcatct
     2221 gcccgcaggc ctctccttcg aagcggctgc cactattccg agcactttct tcacggccta
     2281 ttactccttg cgcacccttg ccggactgcg cgagggcgaa aaggtcctga tccacggagc
     2341 cgccggcggc gtgggcattg cggcgattca ggtcgcgaag tggtgcggag ccgaagtgtt
     2401 tgccaccgcc gggtctgatg agaagcgcaa cttcgtccgg ctgctcggcg ccgatcatgt
     2461 attcgactcg cgcaatcttg ccttcgccga gcaggtcctt aggtgtaccg atgggcgtgg
     2521 agtcgatgtg gtgctcaatt cactcgccgg ggaggcgatc aatcgcaact tccgcgtgct
     2581 taagcccttc gggcgcttcc tggaactggg caagcgcgat ttctacgaaa atacccgcat
     2641 cggcctgcgt ccgttccgca acaacatcag ctacttcggc atcgatgccg atcagctgat
     2701 gaatgagaag cccgaactta cccgacaggt gtttgccgaa gtgctgcagc tcttcaatga
     2761 gcgcgtattt cacgcactgc cctaccagtc ctttgaggcc cgagatgtca tcgacgcatt
     2821 ccgctacatg cagcaggctc gccagatcgg taagctggtg gtcacctacg gcagcggcat
     2881 accggatcct gcttgtacca gcgaaggtcc ggttcaacgt ctcgagctgt cgccatcggc
     2941 cacctatctg atcactggcg gcatcagcgg ctttggtctt cgcaccgccg agtggctggc
     3001 cgatcgcggc gcgcgcagcc tggtgctgct tggacgccgc ggcgcaggag cggacgaggc
     3061 tcagaccgtg atcgcgcgcc tggagcgtca cggagtgcac gtcgcggcat gggcttgcga
     3121 cgtcaccgat cggcttgcac tccacgatgc gcttgagcgg atagcgcgtg agctgccgcc
     3181 attgcgtggc gtcgtgcatg cggcaacggt catcgatgac gcactcattc gcgaaatgag
     3241 cgaggcgcag cttcggcgtg tgatgtcccc gaaggttctc ggagccttgc acttgcacga
     3301 actgacccgc tcactgccgc tcgatttctt cgtgttgtac tcctcggcca ccacgctgtt
     3361 cggcaacccc ggccagagca actacattgc agccaatgcg gtactcgaag cgcttggacg
     3421 ggcgaggcgc gcggccgggc tgccggctac ctgtgtgcgt tggggtgcga ttgatgatgt
     3481 gggttttctg gcccgaagcc ccaagctcaa gcaggcgctg caggatcgga tgggtggttc
     3541 ggcgctgcag tcggcggtag cgctggatgt gctggaagcc gtactgttgt ctggccgatc
     3601 cgacctcggg gtactggaac tggactggcg ggtgatgcgc cgctctctgc cgagcgccgc
     3661 ggcgtcgaag tttgttgaaa tcgcgcgtgg catccttggc gaggtcgaag acgacgcgag
     3721 cttggacctg gatcgcatgc tgcgcgaact gcctgccgag cagttgcagg acgccgtcac
     3781 ggacctgctg cgtgccgagg tgggggagat cctgcggatt gctccggaca aaatcgatgc
     3841 gctgcgtccg atgcaggaga tggggttcga ctcgttgatg ggcgtggaac tggtcgtcgc
     3901 cgtcgagaac cgcttcggcg tgcggttgcc cgtgctcgtc ctcagtgaca gcccgacggt
     3961 gtcgaaactt gcagcgtgga tcatcaagca gcttcgcagc gaggaaggtg ccgcgagcgt
     4021 cgctcaggcg gatgttaccc gcgcccagat cgagcgaatc gcgagtcagc atgcggtgaa
     4081 cacgccgagt gccgccgaaa tcgagcggat cgcgaccgag ctgcgcagcg acgagtccgg
     4141 agcgactcgc cgcatgattc actagtcata ctccgaaagc gacatgaacc gcaaaaccct
     4201 ccccggcctg accgcgaacc tgaaagagaa gctgatccag caaagtctcg aacgccgatt
     4261 gaatcaggcg agcgcatccg cagcgcaggt gcgcacgctg caccaaaatg gcgccagcaa
     4321 tgacatcccc gaggactttt atcgtttcga ccggcatcct ggctaccagc agctgcgcat
     4381 cattgcggat ggcgcggcgc gtatggagat cggcagcccc ttcttcaagg tgcacgaagg
     4441 tatcgccggt gcccgcaccc gtatcggcgg acgtgaatat ctcaacttct ccagctacaa
     4501 ctacctgggc ttgtcgggcc accccgcggt actggatgcg gcgaaacaag cgatcgatcg
     4561 ctacggcacc tcggtgtcgg caagccggcc ggtctcgggt gagcgcgcat tgcatcgcga
     4621 actggaatcc gaattggcgc gagcctatgc agtggacgcc gcggtggcct tcgtcagcgg
     4681 tcatgccacc aacgtcacga ccatcggcta cctgttcggg ccgcgcgacc tgatcgtcca
     4741 cgacgaattc atccacaact cgtcgcttca gggtattcag ctctcgggcg cacggcgact
     4801 gagcttcccg cacaatgact gggcggcgct ggatgcactg ctgaaggagc accggcggaa
     4861 attcgagcgc gcgctggtgg taatcgaagg catctacagc atggacggcg attttccgga
     4921 tctgccgcga ttcgtcgata tcaagcagcg ccacaactgc tttctgatga tcgacgaagc
     4981 tcactcgttc ggagtcatgg gcgaacgggg actggggatc cgcgaacact tctcgctgcc
     5041 aggcgacgca gtggacatct ggatgggcac cctcagcaag accctggcag gctgcggcgg
     5101 ctacatcgcc ggtgagaccg cgctgatcga gcacctgaaa tttctggcac caggtttcct
     5161 ctatagcgtc ggtatggcac caccggtcgc agccgcctct ctggtcgcac tacgctgcct
     5221 gcgagctgaa ccagcgagag tggcgacact gcatgaacgc ggcgcgcagt tcctcgcact
     5281 ggcgcaggcc gccggcatcg acaccggttc gagtgccggt attgcggtca ttcctgccat
     5341 catcggcagt tccacgcgcg cggcgcgcat ttccgctgag ttgatgcatc gggggatcaa
     5401 cgtgcagccg attctttatc ccgccgtacc agaaaagctc gcgcggctgc gcttttttat
     5461 ctgctgcgaa cacaccgaag cggatatccg cgaggcggtc ggcgcactgg ccaccgaagt
     5521 gcgccgttga ttcacggctg gcatgaaccg cgtgcctccg aatccggcag tactctgcca
     5581 actgtatctg gacgcggtac tgccttgtct gagcgagctg gttgctgagg atgccttcgc
     5641 gcgtgatctc accgccgggc tgaatgcttc gatagtcttc cgcgtcgcag gcgggccggc
     5701 gcgctcggtt catctgcgcg acggcgccgt cagctctcaa ccggggagtt gctcccaccc
     5761 gtcgatcgtc ctgttgtttc tctcccgcgc gcatctgaac gcgttctttt cgggacgaaa
     5821 gtgggcgctg ccgcttttgg cctggggcgg atggcggatt cgtgtgctca aggggttctc
     5881 gacactggcg gagcgcctcg aggccgtgct ggaaggccat gaggcggtaa tcagcactgc
     5941 cgccggccgg cgcctgcacg cgcgtctgtc attgatggcc gctggcctgg gactcccatc
     6001 cctggcggcc ggcgatgtgg tgtcgcggga ggcttttcga tctttgccat atggcatggc
     6061 ctcgttcacg ataagctccg agcagggagc cacggtatgg ttcgatcgcc agctggatac
     6121 gagccgggcg ggctggagtg caccaccgcg acaggccgag gtctgcatcg ccttcggcga
     6181 tgcggaaacc gctttcgcgg caatgcgcga tcagatcgac acgatggccg ccacagcatc
     6241 cggacagatc gtcgtggacg gtctgattcc cctggccgat gggctcaact tcatcatgca
     6301 gcgactgcgc gtctatcttc agccctcgaa gtcatgaccg ctaccaagga tcgctaccac
     6361 cgttcactcg agctctttga gagagcctct caggtcatcc catgcgggat ctacgggcac
     6421 acagctcccg ccgccgccct gccgggcgca ttcccctact acgcccagcg cgcggcgggc
     6481 agccgctatt gggatgtcga tggcaacgag tacatcgact acatgtgcgg ctatggaccg
     6541 gtggtcctgg gtcaccagca ccccgagatc gaggaggcag cggtacgcgc gcaacgcgac
     6601 accctgtgcg tgaatcatcc cacgccgatc atggttgagc tcgccgaatc gctcgtctcg
     6661 ctggttgatt tctccgcctg ggcagtactc ggcaaaaacg gttcggacat gacgagctgg
     6721 gcgatccagg tcgcccggga gcacaccaag cgccgcaaga tcctcaaggt ctcaggggcc
     6781 taccacggca tggacccgtg gtgcaccccg gggcatgccg gcctgatcga agaagaccgg
     6841 gtgcacatac acgacttcaa ctggaacgat ctggactcct tcgatcggtt gctgaagcgc
     6901 tatcgcgacg acatcgcggg cgtcatcatg acgccgtttc accacccggt gttcaacgcc
     6961 tcacagatgc catccgacgg gttctggccg cacatcgaag ccgcctgtcg tcgcgacggc
     7021 atcgtgctca tcctggacga catccgtgcc ggttttcggc tggacttgcg cggctcgcac
     7081 aagctgttcg gcttcgaacc agacctgatc tgcttttgca aggcaatcgc gaatggtcat
     7141 ccgctcgcgg ccacgctcgg tcgaaaggaa atgcgtgtag ccgccagcaa ggtgtttctt
     7201 accggaagct actggaatag cgccccggcc atggccgcgg ctatgaagtg cctggcgatc
     7261 ctgcgcgcgc aggatgtacc ttcgcaattg caccgcaccg gtgaaagcct gatgcagggc
     7321 ctggtggagc ggggtcggcg ccacggttac accgtgcagc cctcaggacc tgctgccgtg
     7381 ccgttcatca ccttcgactc ggatcccagc atgcggcgcc ggcagcgttt ctgtgccgag
     7441 gtcacacgac gcggcgcttt cttccatccg catcacaact ggtttatctg cgcggctcac
     7501 tccagcgagg acataaagac cacattggca atggccgacg aggcttttgc ggtgctgcgc
     7561 gccgagaatc tctaaccagt ggaatgagaa atggcaattg tagcgctcaa gaaccatccc
     7621 cggcttagca tcgaggtaca tgcgacaccc gataagttca tcagttcaca gatcgtcgag
     7681 acgggcgagt gggagccgtt cgagacgcat ctggttgaat gtttccttcg ccccggagat
     7741 gtgtttgtcg acataggagc aaatataggc tggtatacca tcatcgctgc gtcgatagtg
     7801 ggcaacaatg gatatgtcta tgcattcgaa ccgggaacag ccaatttcga attggccgcc
     7861 cggaacctgg ttctaaatga tcttcagaac gtcacgcttg aacagatcgc aatcgccgat
     7921 cgcactggca cgggtcggtt gttcctctcg cgggagaatc ttggtgatca caggttgttc
     7981 gcagcggagg aagagcgctg ttcacaaaca gtgtcggtga catcactcgg cagatacttt
     8041 gaacacaacc caacacctat tcggatgctg aagatcgatg cacaaggtag cgaagcaaga
     8101 atctttgagg gcctcccaga cgatttcgct cgaacgcgaa aggtagcggc gatccttctg
     8161 gagttttggc cctatgcgtt ggaacaatcc ggatcgagcg ccaaatctct tgtccgaagc
     8221 ctggcagccc aaggcatgag ttgctacatc atccacgaat gctttcgggg gctcgatcca
     8281 atcgacctag atgttctcga gcagcgcgca tatggtgatt tgcgggaaaa taatctattc
     8341 ctcaatttgc ttgccttgcc tgccacaaac gcggttcccg ctgctattcg cacaatgatc
     8401 aggccgcctg atgcgttcct tttctaccac gctgctcgtt gatggtaaat tgacgacggg
     8461 ccctaccgac catgcctcaa gccgtaatac ttgcgcagtc cccagtgtac ccacgcacgc
     8521 aggctgcgcc gcgcaagcgc gatagcgatg cgcgcctgaa ataaatcccc gaccgcgacg
     8581 gcgggcctgc gaatttcacc atcaatgatc cgcacgaccg cgttcgccaa gagctgcgga
     8641 tcgggtgcgc gtgccatgtt gcgatcaatc gcgcgccagg caatctcaag atttggcgcg
     8701 tacgcgtccg acaaccggtc accgaccctg cgggtcgaag gatggaacgc cgtcgcgaaa
     8761 tcaccagggc gaacatccac cacccgtatg ccggtgtgcg caagctccag gttcaggcca
     8821 tcgctcagcg tgctcagcgc tgacttgccg gcgctgtacg gcgcgagaaa cggtaccggc
     8881 aactcccctg ccagtgatga gatattgatg atcagcccac ggtcactcgc ccgcatggcg
     8941 ggcagcgcca gggcgatgag gtgcaagggt cccaccagca gtgtctgaaa ctgcgatcgc
     9001 agctcgtcgc aggtaaagcc ttctatcggc ccgaacatgc cggcaccggc gttgttgatc
     9061 agcacatcga attccccagc agccgcgagc gcctcgcgga atccagcgtc aatggaacca
     9121 tcatcattga ggtccaggac gacgccacga aaaccggaga gatcaggcag ccgctcgcgg
     9181 ctgcgactgg taccccagac ctgatgtccg cgctgacaca acgcctttgc gacgcagagg
     9241 ccaatgcctg aactaacgcc cgtgagaaag atgcgcttca aagcgatcta cggaagatcc
     9301 aaccgactcc aggtggtgcc ggtacgaacg acccgcgctg gtcggcccac gcttaccgta
     9361 ttggccggca actcggcatc cttcaccagc gaacccatac cgacgacgct gtccgccccg
     9421 acatggcagt ctcccatgac ctcggcgtct cgacacagcc acacatggga ttcgaggatg
     9481 atcacaccac ccatcttgtt gatgcgcttt ccagtcgcaa cgtcgcggat ggcatgactg
     9541 tcgtccgtgt acagacgaac gcctattgcc cacatgccgt cggccccgac ggacaccaca
     9601 ccgccattac gagcatctac ttcggcccac atcgtggccc cggtcctttc gccgatgagg
     9661 atcgtgcaat agccaatggc gctcatagcc ccggactgga tctggacctt gtcgcccaga
     9721 acaaccagtc ctccctcgcc tgctacgttg acatgattga tggccgcctc ctcaccgacg
     9781 accagaatca catttcgagg cagcggcaac tcaaaaaaac cggcccgcag ggtgggagtt
     9841 ttggatttcg gtgaggcgag gagcacgttg tttcccgcgg tccaccagtc cggcaagccc
     9901 tcgcgcctca tccgcagatc cgtgacctgc ggcagcctgg gatgcgattt gacctgggcg
     9961 cactcgaggc gtcgcccgat ctctaccgtc aatcggtcgc gcacctgctc gtcgcatttg
    10021 tcgccatcgc gcaggtgctg ggtcgcttca aagaatgacg gtaccccatc ggtgcgcgtg
    10081 acctgatggg gcttgcgggc gacgataacc tgcgatttcg gaagaaattc gtctagcact
    10141 gtctgcacat gacgtgcctg ctcactcttg agcacctcag cccaggttcg agcccagaaa
    10201 tccagactcg ggtggctgga cgcattcgac agcggcacat tcgcctgcca gaacagcgcc
    10261 atccagatgg accagaagaa cccttgcgac atcgtgtgct cgatcgtgag cccgctgccc
    10321 tgaaccagcg ccgcaaaact ctgcggttcg aatatgcgta cgtggtttgg cttctcgaaa
    10381 tagctcggat gcgcgagtcg cttttgcagc atttcctgcg cagttcccgg cacggtgatc
    10441 aggtacaacg cccccggtcg gccgacccga aacaactccg ccatcaccgc tgtcgggtca
    10501 tcgacatgct cgaggacctc agtgcagatg acacggctga cactctcaga ggtcagcggc
    10561 agtcggtgcg catcactgac atgcgtttcc agccgcagtc cgtgatagcg tgccaatcgc
    10621 gcgcgcgcct gtcccaggtt cgctggattg gaatcaatca gaatgatctg cgagccgcgt
    10681 tgcgcacaaa aggtggccat accgccgatg ccacatccga catccagcac cacatcctcc
    10741 gagctcacgg gaaacccgtc cgtcagttct cccttctcct ggttgtacca accggcaagc
    10801 gtatcgttct tcagaccgac cagggcgctg aagccgatat ctttcagttc gtccaactcc
    10861 gcgctcatgt cgccaccctg gaaagcacgc gacgcatgaa agtcgctcga tttgtgcgcg
    10921 ggcgcaagcc cagatgctcg aagacttcac gcagccggct gctgacgact tcatccgaac
    10981 agaatcgttg ctgggccgca ctcgccgcag cggacattgc cgtatatcgc tccgactggt
    11041 tcttggcgac atcgaaactg tcccgatagg cctggaccag ctgtgaaaag ttgatcgcgt
    11101 actgcgtgca gctgattgac tggcgcggat cgtgcggcca ggaggttggc ttccgcgtcg
    11161 agccaaccac aaagctgttg tcgctcgaga cgtagtccag catcgcggtg tgcagcggtg
    11221 taaccgctgg gcgccccgca gacatgaatt ccatcagcgg cagacactgt ccttcgccgg
    11281 tggagacgtt gacggcgtag ctggtgacgt cgaccagcga atcatattcc tcttgcgcaa
    11341 tcatgccgtg aacaatcagc acgcggcact tgaagtcgca atgcttggac aggtccgaga
    11401 gaatcggcac cacaccgacc accgcatcgt agtgcgtgac cttgatgagg agggacgcct
    11461 ctggattatc tcgaaaggcc cagatgaagg ccgccagcag atccgtccag ttcttacgct
    11521 gatccactgg attgaacact gcggtgtaca ccacaccgct caagctgaag cgcagcggtt
    11581 tgttgccgcg cgattgcact ctggcattca gcgccttgac cgccttggag ccgtcggtcc
    11641 tggagcgatc gacattgaag agcgacagat ccacagcgcc ggcatcgatc gcaaaaccac
    11701 ttatcgtcag tgtcgtcgga gactgccacc ccttggccga gactgcacgc cgcacgttcg
    11761 attgatacat cggcgcgggt atgctccaga tgggatagtt ctctccgagg tagcggcgta
    11821 ccacctgcac ggcgaagcga ctgtgcgtga tcgccgcggg tacctgactc agcacggtgg
    11881 tccagtcgtt tctcggctcg ttctgccagg gctcatcggg cagtgtatcg aactcccatg
    11941 cgaataccgg aaccgtggga cacactgacg cgaccatggt gtgatgcggc gggctgaagg
    12001 agaaatacac actcgactcg ccgtaagatg ccgcgttctt cgcgatgcga tcgacctcgc
    12061 gtgccggatc ggttatcgga acgatgatac cgatacgctc gaataccgga cgaaacgcct
    12121 tgcgtacaaa ccaatagctg tagtcagggg cgcccaggcg cccgccgatg gcccgagcat
    12181 cgaatgtgga atgttctaga acgatcatgc cgcgactctc aagccgagga agctggcaat
    12241 gcgttcggca acgcgctcgt cgttgcagaa agtgctcatg gttcgcaggg ccgcattaca
    12301 catggcctca tagcgactcg ggtcagactt tgtgatgcga taggcctcgc gcatcgaatc
    12361 gcgcagcgat tcccactcga tgcgatagcg cctggaattg aaatggcaac gcctatcctg
    12421 aggccacgcg cagtaatcgt catccgagcg cacgatgatc gaattttcgg cgttcagata
    12481 gtcggccatc gcggtgtggc cgggcgcgac caccggccgg cctgcgcaca tgaattccag
    12541 caacggcagg catagcccct cagctacaga ggaattcagt acccagtgtg aggcaccgac
    12601 gagccgtgcg tagtgcgcat cggggagaaa tccctgcagc accactactc ggcagcgtgc
    12661 cggcggcatg cgcgccagca gatcgtgagt ttcccaccac cagtaggact cggggccaat
    12721 catcttgaac accagtgtcg catccggtgt atcaatgaag gccgatagaa atgcggtcaa
    12781 catgtcctgc cagttcttac ggccatcttt gggcgcgaac acggaagtga agaccacccc
    12841 atcgatgctc gccaacacac tcttcgcagg acccttcgga gtgggacgtg gcggtgccgg
    12901 gggtgcttca cccatccgga acgatccttg gcgtgagtcg aacacgaagc catccagcgc
    12961 gagttccgca ctcgaggccg gtgcgcgcag cgccagtttg ctgcccgctg tctgcgagcg
    13021 gtcccagacc ggcgcgggca ccgccagcac gggatactga tcgcccattg cctgtcgtac
    13081 tgcgaccgct gcgtactcgg acaaagtaat tgcccccgcg gtgtgtcgca gcacgcgccg
    13141 ccaatcgtta cgcggctcat cgccccagtt ctcggtcggg atgttcgagt actcccaagc
    13201 gatgacgcac attgtcgggc agcgcgaccc gagcctcatg cgatgaggcg gtgcgtaata
    13261 cacgaacagg cagttctcac ctcgggcagc acattgctcg taaatgcggt tgacctcgtc
    13321 caccggatcg gacgcaacat gaacggtgcc cagacgagcc agtagcgggt tgaatgcgcg
    13381 cgcgacgaag tagtagctgt actcgggacc cccaaagtct ctggccagcg acttctcatt
    13441 ggttgagctg taaacgataa aattgttcgc gaccccctgc cgtgcgcaaa atggcggtga
    13501 ttgcgcctcg gccgccggct cggcactgac cgcactgcgt tttgcggcag tctgattgag
    13561 cacgctctcg atgaaagcgg cgatatccgg ctcagtgact tcgtccatca cgtcgccatg
    13621 ggcgtcaacc aacagtttgg agcccggcag aggatctcca tccttgacgc gcaccacgat
    13681 gcggctcaag cccagccggt ccatatcgag cagcaccggc accgcaaatc cgtgtctacc
    13741 atcgccaacc ccagccactg ccagatcggg tcgatgcagc cacgccgcag cctcgcccgc
    13801 gacgacgcca tcgatcagca cctcgaccgt gagggtttcg tgcggcgtac tcggacgcca
    13861 ggcccagccg cagatgcgcc cttcgcgaac cccgtcgacg tacccctgaa cttctgaatc
    13921 ttcttcgcca gccatcgctg tcatcctacc atcgcacggc acccagattg atccgacctt
    13981 gatccggcgc cttacccggg ctgtaccagg agtagaccac ctgctcgcga gtactcagtt
    14041 ccggatgcag gttgagggca tagcagccaa agacaccgat ggcacaggca tccggtacgc
    14101 gtcctgtcgg gccggcactc caggggcctg ccggcgcggg cgcttcgtac aacgcaaatt
    14161 ctgcggtacg aaatccggtc tgtatcagca tcacgaactt atgtaacgtc gtggcgccaa
    14221 ggtcgctgac gctggctccg aagaccggta tgccgctcac cagcgagctt gccgcagtct
    14281 catccgaact ccaacgcccc tcgctttgag gcggggcact ccaccacgca tagctcgacg
    14341 cgttgcccca cgcggaaggc tcactgctga cgcgagcgac atacaccccg cctaagggct
    14401 cgtgtgcata cagatacaga tatccgtccc caccgaacac cggcgattcc agtgcctcag
    14461 gccgcgccag tccggcactc agtggctttg cgacaaatgg cgtccacgag ctcaggaatt
    14521 gattgtgctg cgggtcatag atcgtcagcg acaggcgttc gaccggaaag gctttggacg
    14581 tcacgcagac ctgaaaatag acgagcaaga ccttggagga tcgcggaatg cgcgcgccgc
    14641 ccgtgggcca ggccgcggga tacagactgc cgctgccgtg cgctccgcag ggcagcatgg
    14701 cgcggcctgc cgagccgggg gcaccaaggc cgaccggtgt cggaaagaac ggctgcggcg
    14761 acggcaaggg cgatgcgggc atcgtgggtg gcgtaggcac ctccgccata tcggtcggcg
    14821 ccttgccggc gaccacggct gcgatcgccg cggtggtccc tccgataaag ggcggaccat
    14881 tcttctgact ggtatcgccg aacagccaca ggcttctggc cggttgcttg gaatactgaa
    14941 cactgaagcc tccgtcgcgg ctgatcgggc gaaggacggt gtcggcgcgt gcatgaaccg
    15001 gacccagcgc agtcgccggt tgctgttcag cggccgctgc cggcaagcac gcgaacatca
    15061 ggcatgcact caccgtcagc aatctgcgca atcagtccgg ctccttgcgc tgcgcgatc
//
LOCUS       DATMRM010000492         7334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3378593, whole genome shotgun sequence.
ACCESSION   DATMRM010000492 DATMRM010000000
VERSION     DATMRM010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3378593"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VNZ06_14980"
     CDS             complement(<1..406)
                     /locus_tag="VNZ06_14980"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22102.1"
                     /translation="MSGPRILVIDGNVGEIRARQIAALGYDSGNGYARVLRRLDPALD
                     IDIVAAADGSLELPPNMGLEGYDGVTMTGSALNFYNGGAPVTRQIELTQAVFAAGVPF
                     FGSCWGLQVAVTACGGEVRANPRGREFGFARRI"
     gene            complement(403..1575)
                     /locus_tag="VNZ06_14985"
     CDS             complement(403..1575)
                     /locus_tag="VNZ06_14985"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HWW22103.1"
                     /translation="MNATLRGNWNYPTSIWAGPGRIADLSAACSRAGMRRPLLVTDEG
                     LSLNPMIKAALAAVEHAALFASVRGNPTSSQVEAGLQAFRAGGHDGVIAFGGGSALDV
                     GKVVAFMSGQTRPLWDFEDIGDWWTRADPAGIAPVVAVPTTAGTGSEVGRAGVILNET
                     THQKKIIFHPQMMPRIVISDPELTVGLPRAVTAATGFDAFIHCFEALCAPGFHPLADG
                     VALEGMRLIHRFLPRACAHGTDIEARAQMLAAASMGATAFQKGLGAVHAIAHPVGSWF
                     NTHHGLTNAIIMPYVMVFNRGAIADKTDLIARVLDLPARGFDGFLECVLQMRRELGIP
                     HSLKEIGVSTDKADIIGREASIDPSAGGNPIPVDAATLERIFRAAVNGNLAAVISA"
     gene            complement(1572..2963)
                     /locus_tag="VNZ06_14990"
     CDS             complement(1572..2963)
                     /locus_tag="VNZ06_14990"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWW22104.1"
                     /translation="MTDVLKAISPVDGRVYVERSLESAAGIERALDRAQRAQRAWRSV
                     PLAVRCDTLQRAVDAFVANAGEIATEITWQMGRPIRHSPAEVRGFEERARCMLQLAPE
                     TLAAIQPGDKPGFERQIKRVPLGVVVVVAPWNYPYLTAVNAVLPALIAGNAVVLKHSH
                     QTPLCAERFLEAFAKAGVPAGVFQYLHLSHADTSRLMSDPRVASVCFTGSVAGGRAVV
                     AATATGFATAGLELGGKDPAYVRADANLAHAIESLTDGAFFNAGQSCCGIKRIYVAAS
                     RYEEFVDGVVELATQYQLGSPLDPQTTLGPVVRTAAADAVRAQVREALAHGASQLIDQ
                     AAFAASIPGTPYLAPQVLVDVDHTMAIMREETFGPAVGIMKVDSDEQALRLMNDSDYG
                     LTAAIYSADAAHAEALGDALETGTVFLNRCDYLDPALAWTGVKNSGRGCTLSRVGFEQ
                     LTRAKSYHFRVTT"
     gene            complement(2972..4339)
                     /locus_tag="VNZ06_14995"
     CDS             complement(2972..4339)
                     /locus_tag="VNZ06_14995"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22105.1"
                     /translation="MTQSPVEQEAGLRNFLTAHDIEHVKIGVFDGDGILRGKYLSRDK
                     FLSALQKNVGFCDVVLGWDSNDQLFDNTTFTGWHTAYPDASVRILPATRREIPFEPRT
                     ALVLGEFAGAAESVCPRGTLRRVLERAAGMGYSVNAAAEFEFFMFEETAHSVREKGYR
                     ALKTMTPGAFGYSVLRSCVHSDLYQELLDLARIMRFPIEGLHTETGPGVLEAALMHCE
                     ALEAADRAALFKTFAKVFAQRRGLMATFMARWSNSVPGQSGHLHISLRGSDGNSVFHD
                     PSQPHDMSDTMRWFVGGQQALMPELLAMVACTVNSYSRLVPGFWAPTTAAWGVENRTT
                     ALRVIRGGPSSQRVEYRIAAADINPYIALAAAIGSGLWGIEHRIEPDAAIQGNAYDRE
                     HPPQRRLPATLYEAAERLAASKPARTLFGDAFVEHYAATRQWEEREFRKAITDWELAR
                     YFEII"
     gene            complement(4395..5672)
                     /locus_tag="VNZ06_15000"
     CDS             complement(4395..5672)
                     /locus_tag="VNZ06_15000"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HWW22106.1"
                     /translation="MLDNLAPIEVAGRLGLAIGLAVFMGLAFEGIYKRERRTSPGGIR
                     TFPLLAALGAMLFLVDSHTLLPYVVGMGAVAICLFALVRAACDADDGPPSLMIPTANL
                     LAYAFGPLALRQPAWIVVSAAVIAVMLFEGREALHRLVQKIDPDEAFTLGKFLILAGI
                     VLPLLPNHPVVAFTPLAPFNVWLALVAISTLSYASYLLQRYLPKSGALLPSILGGIYS
                     STATTVTLARRQKEAGSARTDVAAGIVIATSMMYLRVDVIVAIFNTRLAVTLLPALGV
                     LFGLSLAIGLTQWSRRAKDPSGDPNRISAGNPLQLGTAVTFAALFVTVAIASAWVRGA
                     FGQKGVFVLAAITGTTDINPFVLSLAQGGVSDMTLTALATAILIAVACNNILNGCYAL
                     AFGGGRATLRPASILFGLGALGLIAAALTVRLG"
     gene            complement(5699..6784)
                     /locus_tag="VNZ06_15005"
     CDS             complement(5699..6784)
                     /locus_tag="VNZ06_15005"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HWW22107.1"
                     /translation="MRPIGWLSAAAIGLCGLLCAQQPEQPPALAPVTAGARLEFPRDL
                     GSHDAFGIEWWYITGWLTAPDNEPLGFQITFFRTRVVGAGANPSAFATDQILIAHCAL
                     SDAKRGRLWQDQRVRRAGMGLAQAQSGQTRVWVDDWRLERSNNIYHTSIAADEFALDL
                     TLAVTQPPMLNGQDGYSQKGPAEASASYYYSEPQLHVAGSVTRSGQRSAVSGLAWLDH
                     EWSSQPLDPAAVGWDWTGLNLDDGGALMAFRIRDQEGHTYWSAGSERDAAGHTQRLTA
                     DQLDFKPLRQWRSPRTGVSYPVSWRLQAGERTLELAPLMDDQENDTRLSTGAIYWEGA
                     VRASERGQPVGRGYLELTGYEKPLTLR"
     gene            complement(6787..>7334)
                     /locus_tag="VNZ06_15010"
     CDS             complement(6787..>7334)
                     /locus_tag="VNZ06_15010"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWW22108.1"
                     /translation="AAIWRRPEADEATLTASIRRALGTGDAIELIASAQLRDRSLMLF
                     DRAFAITYALEIIAVAIGLAGVGLAASSTALARRAQFGMLRHVGMLRRQVLAMLASEG
                     VIMSALSAAYGLLLGLGLSLILVYVINRQSFNWSIDLRVPWTQLAVLSLALIAASALT
                     AVWSGRAAMGQDAVRAVREDW"
BASE COUNT         1362 a         2406 c         2352 g         1214 t
ORIGIN      
        1 ggattcgccg cgcgaaccca aactcgcggc cgcgcggatt ggcgcgtacc tcgcccccgc
       61 aggccgtcac cgcaacctgc aatccccaac aactcccgaa gaacggcaca cccgcggcga
      121 acacggcctg ggtcagctcg atctggcgtg tgaccggcgc gccaccgtta tagaagttga
      181 gtgccgatcc cgtcatcgtc acgccgtcgt agccctcgag gcccatgttc ggcggaagct
      241 ccagcgaacc gtcggcagcg gccacgatgt cgatgtccag cgccgggtcc aggcgtcgca
      301 gcacgcgcgc gtagccgttt ccggagtcat agccgagtgc ggcgatttga cgcgcgcgaa
      361 tttcgccgac gttaccgtcg atgaccagga tgcgtggccc gctcacgcgc tgatcacggc
      421 agcgaggttg ccgttcacag ccgcccggaa gatgcgttcc agcgtcgcgg catcgaccgg
      481 gatcggatta cccccggcgg acggatcgat cgaggcttca cggccgatga tgtcggcctt
      541 gtcagtgctg accccgattt cctttagcga atgcggtatc cccagttcgc ggcgcatctg
      601 cagcacgcac tcaaggaaac cgtcaaaccc gcgcgccggc agatccagaa cgcgcgcaat
      661 gaggtcggtc ttgtcggcaa tcgcgcctcg attgaagacc atcacgtagg gcatgatgat
      721 cgcgttggtc agaccatgat gggtgttgaa ccaggaaccc accgggtgtg cgatcgcgtg
      781 cactgcgccg agacccttct gaaaggcggt ggcacccatc gacgcggccg ccagcatctg
      841 ggcgcgcgcc tcgatgtcgg tgccgtgcgc acaggcacgc ggtagaaagc gatggatgag
      901 ccgcataccc tcgagggcaa cgccgtccgc gagcggatga aagcccggtg cgcacaaggc
      961 ctcgaaacaa tgaatgaagg cgtcaaagcc cgtggctgcg gtgactgcgc gcggcaggcc
     1021 gacggtcagt tcagggtcgg aaatgacgat gcgcggcatc atctgcggat ggaaaatgat
     1081 cttcttctga tgggtcgtct cattgaggat cacgcccgcg cggccaacct ctgagccagt
     1141 ccccgcagtg gtagggaccg ccaccacagg ggcgatgccg gcggggtcag cccgggtcca
     1201 ccaatcaccg atgtcctcga aatcccatag cgggcgtgtt tgtcccgaca tgaacgccac
     1261 gaccttgccc acatcgagtg cagagccgcc gccgaaggcg atcacgccgt catggccgcc
     1321 ggcgcgaaag gcctgcagtc ctgcttccac ctgactgctg gtcggattgc cgcggacact
     1381 tgcgaacagc gccgcatgct cgactgcggc cagggcggcc ttgatcatcg ggttgaggct
     1441 cagcccttcg tcggtgacca gtagcggtcg gcgcatgccg gcacgcgagc acgcggccga
     1501 cagatcggcg atccggccgg gcccggccca gatactggtc ggataattcc agttgccgcg
     1561 tagagtcgcg ttcacgtcgt caccctgaag tgataggact tcgctcgtgt gagctgctcg
     1621 aagcccactc gcgacagcgt gcagcctcga cctgaattct tgacgccggt ccaggccagc
     1681 gccggatcga ggtagtcgca tcggttcaga aacaccgtgc cggtctcgag cgcatctccg
     1741 agtgcttcag cgtgcgcagc gtcggccgaa tagatcgcgg ccgtcaaacc atagtcgctg
     1801 tcgttcatca gtcgcaatgc ctgctcgtcc gagtccactt tcatgatccc gaccgcaggt
     1861 ccgaaggtct cctcacgcat gatcgccatg gtgtgatcca cgtcgaccag cacctgcggc
     1921 gcaaggtacg gggttccggg gatcgaagcg gcgaatgcgg cctggtcgat caattgactg
     1981 gccccatgag cgagcgcttc gcgcacctgt gcgcgaaccg cgtcggctgc ggcggtgcgc
     2041 accacgggtc cgagggttgt ctgcggatcg agcggtgaac cgagctggta ctgcgtcgcg
     2101 agttcgacca cgccgtcgac gaattcctcg tagcggctgg ctgcgacgta gatgcgtttg
     2161 atgccacagc atgactggcc ggcgttgaag aaggcgccgt cagtcagcga ctcgatggca
     2221 tgcgcgagat tcgcgtcggc gcgtacgtaa gcaggatctt tgccgccgag ttccaggccc
     2281 gcggtcgcaa accctgtagc ggtcgcggca acgacggctc tgccgccagc caccgagcca
     2341 gtgaaacata cgctcgcgac gcgtggatcg ctcatcaggc gcgaggtgtc ggcatgcgac
     2401 agatgcaggt attggaatac gcccgccgga acaccggcct ttgcgaaagc ctccagaaag
     2461 cgctcggcgc acaacggggt ttgatgcgaa tgcttgagca ccaccgcatt gccggcgatc
     2521 agcgccggca gcaccgcatt gaccgcagtc aggtaggggt aattccaggg cgccacgacc
     2581 acgaccacac cgagcggcac gcgtttgatc tggcgctcaa atccaggctt gtcgccgggc
     2641 tgaatggcgg cgagcgtctc cggagcgagc tgaagcatgc agcgcgctcg ctcctcgaaa
     2701 ccgcgcacct cagcgggtga gtggcggatc gggcgtccca tctgccaggt gatctcggtg
     2761 gcgatctcgc cggcgttggc gacgaaggca tcgaccgcgc gctgcagcgt gtcgcaacgt
     2821 accgccagtg gcaccgatcg ccaggcgcgc tgcgcgcgtt gtgccctgtc cagcgcgcgc
     2881 tcaatgccgg cggcgctttc gagcgatcgc tcgacgtaga ctcggccgtc gacggggctg
     2941 atggccttaa gaacgtcagt catgagtcaa ctcagatgat ttcgaagtag cgggccaatt
     3001 cccagtcggt gatcgccttg cgaaactcgc gctcctccca ctgtcgggtc gcggcatagt
     3061 gttcgacaaa ggcgtcgcca aatagagtcc gcgccggctt ggatgcggcg aggcgttcgg
     3121 cggcctcgta gagcgtggcg ggcaggcgcc gctgcggcgg atgttcgcga tcgtaagcgt
     3181 taccctggat cgcagcgtca ggctcgatac ggtgctcgat gccccagagc ccggaaccga
     3241 tggccgccgc gagcgcaatg tacggattga tgtccgcggc cgcaatgcgg tactcgacgc
     3301 gttggctgga cggaccgcct cgaatgacgc gcagcgccgt ggtgcggttc tcgacccccc
     3361 aggctgccgt ggtcggggcc caaaaccccg gcacgaggcg cgagtagctg ttcaccgtgc
     3421 aagcgaccat cgcgagcagc tcgggcatca gtgcctgctg gccgcccacg aaccagcgca
     3481 tggtatcgga catgtcatga ggctgcgacg gatcgtggaa cacgctgttg ccatcgctgc
     3541 cgcgcaatga gatgtgcagg tggccgctct gtccaggaac gctgttggac cagcgggcca
     3601 tgaaagttgc catcaagccg cggcgctgcg cgaatacctt ggcgaaggtc ttgaacagcg
     3661 ctgcgcgatc ggccgcctcg agtgcctcgc agtgcatgag cgcggcctcg agcactcctg
     3721 gaccggtttc cgtgtgcagg ccttcgatcg gaaatcgcat gatccgcgca agatcgagca
     3781 gttcctgata gagatccgag tgcacgcagc tgcgaagcac cgagtaacca aaggcgcccg
     3841 gcgtcatcgt cttgagcgct cggtagcctt tttcccggac cgaatgcgca gtctcttcga
     3901 acatgaagaa ttcgaattcc gccgcggcgt tgacgctgta gcccatgcct gcggcccgtt
     3961 ccagcacgcg tcgcaacgtg ccgcgcggac agaccgactc ggcagctccg gcgaactcgc
     4021 ccagcacgag cgcggtcctc ggctcgaacg gaatctcgcg tcgggtggcc gggaggattc
     4081 gtacgctcgc atccggatag gcggtatgcc agccggtaaa cgtggtgtta tcaaacagct
     4141 gatcgttcga gtcccagccg agtaccacgt cgcagaatcc gacgttcttc tgaagcgccg
     4201 agaggaactt gtcgcggctc aggtattttc cgcgcaggat cccgtcgcca tcaaacacac
     4261 cgatcttgac gtgctcaata tcatgcgcgg tcagaaagtt acgaagcccg gcttcttgct
     4321 cgacaggcga ttgcgtcatc gaagattggc gtccattgct tcgggcggca aacgacgacg
     4381 cgattgtact agatctagcc caagcgcacg gtgagcgccg cggcgatcag tccgagcgca
     4441 cccagaccga acaatatcga ggccgggcgc aacgtcgccc gcccgccacc gaacgccagc
     4501 gcgtagcagc cgttgagaat gttgttgcag gcgacggcga tcaggatcgc ggtggcgagg
     4561 gcagtcagcg tcatgtcgct cacgccaccc tgcgcgaggc tcagcacgaa cggattgatg
     4621 tcagtcgtgc cggtgatcgc tgccagcacg aatacacctt tctgaccgaa ggcgccgcgc
     4681 acccaggcgg atgcgatcgc caccgtgaca aacagcgcag cgaaggtcac cgcggtaccg
     4741 agctgcagcg ggtttcctgc gctgattctg ttcggatcac ctgacggatc cttcgcacgt
     4801 cgcgaccatt gcgtcaggcc gatcgcgagc gacagcccga acagcacacc gagcgctggc
     4861 agcaacgtca ctgccagccg ggtgttgaat atcgcaacga tcacatccac gcgcagatac
     4921 atcatcgacg tggcgatcac gatgccggcg gccacgtccg tgcgagcact gccggcctcc
     4981 ttctgccggc gcgccagcgt gacggtggtc gcggtcgagg agtagatacc gccaagaatg
     5041 gagggcaaca gcgcgcctga cttcggcagg tatcgttgca gcaggtagct ggcatacgac
     5101 agggtcgaaa tcgcgaccaa ggccagccac acattgaacg gtgcgagcgg cgtgaaggcg
     5161 acaaccggat gattgggcag cagcggcagc acgatgccgg caaggatcag gaatttgccg
     5221 agggtgaagg cctcgtccgg atcgatcttc tgtaccagcc gatgcagcgc ttcgcggccc
     5281 tcgaacagca tgacggcgat caccgcggcg ctcaccacga tccaggccgg ctgccgcaat
     5341 gccagcgggc cgaaggcata ggccagcagg ttggcggtcg gaatcatcag gctcggaggc
     5401 ccgtcgtccg catcgcaggc ggcacgcacc agcgcgaaca gacagatcgc aaccgcgccc
     5461 atgccaacca catagggaag cagcgtgtga ctgtccacca ggaacagcat cgcgcccagg
     5521 gccgccaaca gcggaaaggt gcgaatgcct ccggggctgg tgcgccgctc gcgcttgtag
     5581 atcccttcga aggccagccc catgaacacg gccagtccga tggccagtcc cagccggccg
     5641 gcgacttcga tcggtgccag attgtccagc atgcctgttg tccttgttgt agtgcggatt
     5701 atcgcagcgt cagcggtttt tcataccccg tcagttccag atagccgcgc cccacgggct
     5761 gaccacgctc gctggcgcgc actgcacctt cccagtagat ggcgccagtg gacaggcgtg
     5821 tgtcgttctc ctgatcgtcc atcagcggcg caagctccag cgtgcgctca ccggcctgaa
     5881 gccgccagct caccggatag ctcacgccgg tgcgcggcga gcgccattga cgcagtggct
     5941 tgaagtcgag ttgatcggcc gtgaggcgct gggtgtggcc ggcggcgtcg cgttcgctac
     6001 cggcgctcca gtaggtgtgt ccctcctgat cccggatgcg aaacgccatc agtgcgcctc
     6061 cgtcatccag gttcaatcca gtccagtccc agccaacggc ggccggatcc agaggctgac
     6121 tggaccactc gtgatccagc caggcaaggc cactgacagc cgagcgctga ccggagcggg
     6181 tgaccgagcc cgccacgtgc agctgcggct cgctgtagta gtagctggcg ctcgcttcgg
     6241 cgggcccttt ctggctgtaa ccgtcctggc cgttgagcat cggtggctgc gtgacggcca
     6301 gtgtcagatc gagcgcgaac tcatctgccg cgatgctcgt gtgatagatg ttgttggagc
     6361 gctccagacg ccagtcatcc acccagaccc gggtctgacc gctctgtgct tgcgccagtc
     6421 ccatgcccgc gcgccgcaca cgctggtcct gccacaggcg cccgcgcttg gcatcactga
     6481 gcgcacagtg cgcgatcagg atctgatcgg tcgcgaaggc actgggattc gctcccgctc
     6541 ccacgacgcg cgtacgaaaa aaggtgatct gaaagccgag cggctcgtta tcgggtgccg
     6601 tcagccaacc ggtgatgtac caccactcga taccaaaggc gtcgtggctg ccaagatcgc
     6661 gcgggaactc gagcctggca ccggccgtca cgggcgccag agcgggcggt tgctcgggct
     6721 gctgcgcgca caacagcccg catagtccga tcgcagccgc cgagagccac ccgatcgggc
     6781 gcacgctcac cagtcctcgc gcaccgcgcg caccgcgtcc tgacccatgg ccgcccgtcc
     6841 gctccacacc gcagtcaggg cggaagccgc gatcagcgcg aggctgagca ccgcaagctg
     6901 cgtccacggc acgcgcaggt cgatactcca gttgaaggac tgacggttga tcacgtagac
     6961 caggatcagg ctcaagccca gacccagcag cagtccatag gcggccgaca gtgcactcat
     7021 gatgacgcct tcactggcca gcattgcgag cacctgccgg cgcagcatgc cgacgtgtcg
     7081 caacatgcca aactgcgcgc gccgggcaag tgccgtggag ctggcggcaa ggcccacacc
     7141 ggccagtcca atcgccaccg cgatgatttc gagcgcatag gtaatcgcga acgcgcgatc
     7201 gaacagcatc agcgaacgat cgcgcagctg cgcgctggcg atcagctcga tcgcatcgcc
     7261 ggtccccagc gcccggcgga tcgatgccgt cagtgtcgct tcgtccgcct ccgggcgtcg
     7321 ccagatggcg gcct
//
LOCUS       DATMRM010000493         4148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2463820, whole genome shotgun sequence.
ACCESSION   DATMRM010000493 DATMRM010000000
VERSION     DATMRM010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2463820"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VNZ06_15015"
     CDS             <1..1009
                     /locus_tag="VNZ06_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22109.1"
                     /translation="WGLRARTLKSFILLPLLLAAPCALLAQTPAAPSAPEFLADGLSI
                     RPQNGQAPQQQVTDRTECEAWSKGQTGFDITQPTGGVAPSDYTSRREQFNRAMTACLE
                     ARGYAVRIAAPVTPPASAAPRYSVPAPAAPTYVTHYSPPEPTLKYHPFSVSISGGWNI
                     TTGTVNQNYDDGGLGALAFSLFPAESLPIGLRLDGSYSWFGARDQFLIANNAQLGHQE
                     VYGADADLQFNLGPHTARSQLYLFGGAGWYRERTTLHQVSAVNGTICGFFFCGSSDFF
                     AITGTARATTPWRDSWNAGLGWEIAMSDVSSFFVEARYRQIGPNDSKEAFVPIQAGFR
                     F"
     gene            1055..2566
                     /locus_tag="VNZ06_15020"
     CDS             1055..2566
                     /locus_tag="VNZ06_15020"
                     /inference="COORDINATES: protein motif:HMM:NF024275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22110.1"
                     /translation="MALIACRECTHDISDKASRCPHCGAPASMSAARRRRLRRIAYAL
                     LAVLVVAWGGLTALWLSGRITSPSQIADLFRSHDGGVHAIGPPASAPSAAPIARTAPD
                     SAPQSVYQTSAEQLYQDYNANAVATQSRIGSSRVRITGSVADINQDSVGHPILALWTS
                     SDARVDMVLNDDQRAAVAQLSKGDAVDIQCDHMLRIASAPHGSDCALALIDADARQVY
                     LAVFLSSSSGSAPAYVVGPMPQGFCLSRQDSLSAQLNANPKSDHIASRDCTATARERI
                     ALDGCHLSTTMSAIPEVPGAHLWKYDCGATTVLTARKSKESEQASPRRKSSDAAESEL
                     AADAEQDFTAKDAPKSASSVPPVAPVVAESSPSPSTPPQTTVPPNAAAASTATATSPS
                     PTTPAATSTAATPAPVASAAQPDDLGSVRATDPGAADHIVSYCNAATASATDRASVSA
                     GCRHAEAGAWTRLVLNNEFPTMDDAIRRKCNEPPFPDSYVAKESCAKYQLHVN"
     gene            complement(2667..3761)
                     /locus_tag="VNZ06_15025"
     CDS             complement(2667..3761)
                     /locus_tag="VNZ06_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22111.1"
                     /translation="MRHPNLVAISVSSILAGTLSAAFAAQVQPADPNAQAQVQPQTPL
                     PGVPPVQQASKPVLNAAPSETRADQDQTPDQNRDQTKDQRKDQRKDQRKSDTDAQRDK
                     ADIQADRKDVAKDRADIRADQRDLRNDYAKQRNGVNEQADIQHDKADIQADRKNVAKD
                     RADIRSDQRDLRGDDAARATSDRRDVARDQADLRADRSDIAKDRTDIRSDQRDLRNDY
                     AKQRNGVNEQADIQHDKADIQADRRDVAKDRADIRADRGDLRADHQDLRAAEHGDLRN
                     GVSSRQSEDMRYSDSNRGAATNDSRTLTAMNSAHSPSDRSLHSDVTHPQDLRTPGVSA
                     QTLANNTMENQKQQPSKDSVKKPWYHLWWW"
     gene            complement(3895..>4148)
                     /locus_tag="VNZ06_15030"
     CDS             complement(3895..>4148)
                     /locus_tag="VNZ06_15030"
                     /inference="COORDINATES: protein motif:HMM:NF021515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2242 domain-containing protein"
                     /protein_id="HWW22112.1"
                     /translation="QKMKQTTSMGVGPATLTMPSGSAEVLGTVRRETIHDAEFYKRFF
                     AVVSGMADQERVTQRRAARADRDPPDRADLPADAERQSH"
BASE COUNT          703 a         1367 c         1218 g          860 t
ORIGIN      
        1 ctggggactg cgcgcccgaa ccctcaagtc attcattttg cttccgctgc tgctcgcagc
       61 gccgtgcgca ctgcttgccc aaacacccgc agcaccgagc gcccccgaat tccttgccga
      121 cggcctgtcc atcaggccac agaacggcca ggccccgcag cagcaggtca ccgatcggac
      181 tgagtgcgag gcttggtcca aaggtcagac cggcttcgat atcacgcagc ccacgggtgg
      241 tgtcgctccc agcgattaca catcgcgccg cgaacaattc aatcgggcga tgactgcctg
      301 tctcgaagct cgtggctacg cggtgcgtat tgcggcaccc gtcacgccgc cagcttccgc
      361 ggcaccgcgt tacagcgttc cggcgccggc agccccgacg tatgtgacgc actactcgcc
      421 cccagagccg acgctcaagt accacccgtt ctcggtatcg atcagcggcg gctggaacat
      481 cacgaccggg actgtgaatc agaactatga cgacggcggg ctcggcgcac tggcgttcag
      541 tctcttccct gcggaatcac tgccgatcgg tcttcggttg gacggcagct actcatggtt
      601 cggcgcgcgc gatcagtttc tgatcgcgaa caatgcgcag ctcggacatc aggaagtgta
      661 cggcgcagat gccgacctgc agttcaatct gggtccgcat acggcccgct cgcagctgta
      721 cctgtttggc ggcgccggct ggtaccgtga gcgtaccacg ctgcatcagg tctccgcggt
      781 gaatggcacc atttgcggct ttttcttctg cggatctagt gatttcttcg cgattaccgg
      841 caccgcacgc gcgacgactc cgtggcgcga ctcatggaac gcgggattgg gttgggaaat
      901 tgccatgtcg gatgtgtcca gcttcttcgt cgaagctcgc taccggcaga taggacctaa
      961 cgacagcaag gaagctttcg tgccgattca agcggggttc cggttctagt caagcagtca
     1021 gccgacgcgg ctgcacagag caggacgggc tctcatggcg ctcattgcct gtcgggagtg
     1081 cactcacgac atttcggaca aggcttcgcg ttgcccgcac tgcggcgcac cggcctcgat
     1141 gtccgccgcg cgcaggcgcc gcctcaggcg catcgcctat gcgctgctgg cggtactcgt
     1201 cgtcgcctgg ggcgggctca cggcactgtg gctgagcggg cgcatcacct cgccttcaca
     1261 gatcgctgac ctgtttcgtt cgcacgatgg cggcgtccat gcgatcggcc cgcccgccag
     1321 tgcacccagc gccgcgccta tcgcacgcac cgcgccggac tcagcgccac agtcggtgta
     1381 tcagacctcg gcggaacagc tctatcagga ctacaacgcc aacgcggtgg caacgcagag
     1441 ccggatcggc agcagtcgcg tgcgcatcac cggcagtgtc gctgatatca atcaggattc
     1501 ggtgggacat ccgatcctcg cgctgtggac cagctcagat gcccgggtgg acatggtcct
     1561 gaatgacgat cagcgtgccg cagtggcgca actctccaag ggcgatgcgg tggacatcca
     1621 gtgtgatcac atgctgcgca tcgcctccgc gcctcacggt agcgactgcg ctctggcgct
     1681 catcgacgcg gacgcccgac aggtgtatct ggcggtgttc ctttccagca gtagcggcag
     1741 cgcgcccgcc tacgtcgtcg gaccgatgcc gcagggcttc tgcctgtcgc gccaagacag
     1801 cctgtccgcg cagctcaacg ccaacccgaa atccgaccac attgcatcca gagactgcac
     1861 cgcgaccgca cgcgaacgta ttgcgctcga cggctgtcat ttgagcacca cgatgtctgc
     1921 gataccggag gtacccggcg cacacctgtg gaaatacgac tgcggtgcaa ccaccgtcct
     1981 gacggcacgc aaatccaagg aaagcgagca ggcttcgccg cgacgcaaat ccagtgacgc
     2041 cgctgagagc gagctggcgg ccgacgcaga acaggatttc accgcgaagg acgcgcccaa
     2101 atcagcgtcg tcggttcctc cagtggctcc ggtggttgct gaatcatcgc catcgccatc
     2161 gacaccgccg cagaccaccg tgcctccaaa cgcggctgcc gcgtctacag ccacagccac
     2221 ctcaccttcg cccactacgc ctgcagcgac gtccactgct gccacacctg ctcctgttgc
     2281 gtccgctgcg cagccggatg atctgggcag cgtccgtgcg accgatcccg gtgcggccga
     2341 tcacatcgtc tcctactgca atgccgccac tgcgtccgcc accgatcgtg catcggtgtc
     2401 tgccggctgc cggcacgcag aggccggagc ctggacccgg ctcgtgctca acaacgaatt
     2461 tccgacgatg gatgatgcga tccgtcgcaa gtgcaacgag ccgccgtttc ccgacagcta
     2521 cgttgccaag gagtcttgcg ccaaatatca gctgcacgtc aattgagggt acgcgctatg
     2581 cgaccttgcg tgcgggctga acgtagcagc cccgccgccg ccacgaccgc aaatgcggcg
     2641 gtcgtggcgc cccgaaacgc ttcaaactac caccaccaga ggtggtacca gggcttcttg
     2701 acgctgtcct tcgatggttg ttgcttctgg ttctccatcg tgttgttggc caacgtctga
     2761 gcgctcacac ccggggtgcg caagtcctgc gggtgcgtta cgtcggaatg gagggatctg
     2821 tcactcgggc tgtgcgcgct attcatggcg gtcagagttc gactgtcgtt cgttgctgcg
     2881 cctcgattac tgtcgctgta cctcatatcc tcgctctgac gactgctaac accattgcga
     2941 agatcgccat gctccgccgc acgcaggtcc tgatgatccg cccggaggtc gcccctgtcc
     3001 gctcgaatat ccgcacgatc ctttgcaaca tccctacgat cggcctgaat gtctgccttg
     3061 tcatgctgga tgtcggcctg ctcgttcacg ccattgcgct gcttggcata gtcgttgcgc
     3121 agatcgcgct ggtcggagcg tatatcggtg cggtccttgg cgatgtcgct gcgatccgcc
     3181 cggaggtcgg cctggtcacg agcaacgtcg cggcgatcgc tcgttgcacg cgccgcgtcg
     3241 tcaccgcgca ggtcacgctg gtcagaacga atatccgcgc gatcctttgc aacattctta
     3301 cgatcggcct gaatatctgc cttgtcatgc tggatgtcgg cctgctcgtt caccccattg
     3361 cgctgcttcg catagtcgtt gcgcagatcg cgctggtcgg cgcgaatgtc tgcccgatcc
     3421 tttgcaacat ccttacgatc ggcctgaatg tcggccttat cgcgctgagc atctgtgtcg
     3481 ctcttccttt gatccttcct ttgatccttc ctttgatcct tcgtttgatc gcggttctgg
     3541 tcaggggttt gatcctggtc ggcgcgtgtt tcggacggag cggcgttgag cacgggcttg
     3601 cttgcctgct gcactggcgg tactcccggc aacggcgtct gcggctgcac ttgtgcttga
     3661 gcgtttggat ccgcgggctg aacttgcgcg gcgaaggccg ccgacagtgt cccggcaaga
     3721 attgaactga cactaattgc aactaggttg ggatgcctca tggtgcttcc tcctttccag
     3781 tgttccaata acctaactgg ctgttcctaa caattggccc ttgattggcc cttgagattg
     3841 gcccttgaga ttggcccttg agcgagcaat ctgggtatcc agattcacta gtcgctagtg
     3901 gctctgcctt tcagcatcgg cgggcaggtc agccctatcc ggagggtcac gatcagctct
     3961 tgctgcacgt cgctgagtga cgcgttcctg gtcggccatc ccgcttacga cggcgaagaa
     4021 gcgcttgtag aattcagcgt catgaatcgt ctcgcgccgc accgtcccga gtacttcggc
     4081 cgagcccgag ggcatggtca gcgtcgccgg acccacgccc atgctcgtcg tttgtttcat
     4141 cttctgca
//
LOCUS       DATMRM010000494        10783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3075977, whole genome shotgun sequence.
ACCESSION   DATMRM010000494 DATMRM010000000
VERSION     DATMRM010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10783
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3075977"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VNZ06_15035"
     CDS             <1..342
                     /locus_tag="VNZ06_15035"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22113.1"
                     /translation="TQTHLMDELKKVKRVIARVMPDAPHEVLLILDANQGQNALAQAQ
                     QFHEALGVTGLVLTKLDGTAKGGIVVAIARSLRIPIRFIGIGEKSEDFGVFNASAFAS
                     ALVDGSEAGAS"
     gene            339..989
                     /gene="ftsE"
                     /locus_tag="VNZ06_15040"
     CDS             339..989
                     /gene="ftsE"
                     /locus_tag="VNZ06_15040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HWW22114.1"
                     /translation="MIRFERVSKRYPNGREALCEVSFEIADGEMVFLTGRSGAGKSTV
                     LKLIALLERPSRGQVIVNGDSTARLPLRRVPMYRRRIGVVFQEHRLLMDRPVFDNVAL
                     PLVVADTSFREMEKRVRAALDQVGLLGKERMAPQELSVGEQQRVGIARAIISKPPLLV
                     ADEPTGNLDATLAAEVMGLFKRFNEVGVTVIVATHDLHLVQESGKREIVLDEGRVL"
     gene            992..1924
                     /gene="ftsX"
                     /locus_tag="VNZ06_15045"
     CDS             992..1924
                     /gene="ftsX"
                     /locus_tag="VNZ06_15045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HWW22115.1"
                     /translation="MANPLANWVSRHGHALFSSAGHLVRHGVPTLLTVGVMGLALSLP
                     LALQVLVSNVRDATGDFSGAVGMSIYFKTDVPPQKVQQLAKTAADRAGVASVTLVTAA
                     QALEEFRTQSGFGAALDALTDNPLPNVLTIRPTPFSSTPERLTALRDYFGSWPEVDSV
                     QLDSDWALRFGAILELLRRTVLIAAALLGTGVVAVVGNTIRLEILNRRSEIEVTKLVG
                     GTNAFVRRPFLYAGALYGIAAGAMAWAIVGLARMWLAPAAAHLASAYGSHFALRGPDA
                     RELQVLLGAGVALGWVGAWIAAARQLARIEPRAS"
     gene            2139..3023
                     /gene="rpoH"
                     /locus_tag="VNZ06_15050"
     CDS             2139..3023
                     /gene="rpoH"
                     /locus_tag="VNZ06_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HWW22116.1"
                     /translation="MSAQTALVAHSPGLAGLALAGPIGSFDAYLERVGRIPVLTRDAE
                     QELAQRFRRDNDLDAARQLVLSHLRFVVHIARGYQGYGLPMNDLVQEGNIGLMKAVKR
                     FDPSVGVRLVSFAVHWIRAEIHEYVLRNWRLVKVATTKAQRKLFFNLRRMKKNLTWLS
                     AAETKAVAQDLGVSSAEVSEMERRLSARDLSFDVTAESDEDDGYGPASYLPAADADPA
                     EQVEDEEWSSSNHDQLAAAMQRLDERSRSILQRRWMNDDKATLHELADEYGVSAERIR
                     QIEAGAIAKLKGLLPAPV"
     gene            complement(3040..3534)
                     /locus_tag="VNZ06_15055"
     CDS             complement(3040..3534)
                     /locus_tag="VNZ06_15055"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWW22117.1"
                     /translation="MAPRIAHGQVSRAQQRELDHAESLLHDWLMDLPPNSAVAILRAD
                     DHLTLRFSTSWVFEADGTALKADARRALPVSAALRLLKRRRMLGAQISVYTDSLGGES
                     ANQSFSAARAKALYDCFTAQGISAARLQQRGAGANDALASNGTPEGRIENRRVEIWFA
                     RAVK"
     gene            3619..4827
                     /locus_tag="VNZ06_15060"
     CDS             3619..4827
                     /locus_tag="VNZ06_15060"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWW22118.1"
                     /translation="MSTSTKPAPAAAAAAVPTESAVDYLRLDEELTEDERLVRDSVAR
                     LVDQRVLPIIGECFENSRFPKELLPSIAELGLLGSSLEGYGCAGLNAVCYGLICRELE
                     RGDSALRSFVSVQSSLCMYPIHAYGSEVQKQKWLPRMAKGEVVACFGLTEAHGGSDPA
                     NMKTHAKRSADGWVLNGSKMWITNGYIADLAVVWARTPDGVRGFLVERGTRGFTSEEI
                     KHKFSLRASATGALYFDDVHLPADAMLPAGIGIKAPLSCLSHARFGICWGAIGAAQAC
                     IAEALGHVGERELFGRTLNHNQAVQIRLAEMVRQLALGQLLAMQLARLKDRGELTPTA
                     VSLAKWNNCRMALEIARDCRDLLGGAGISSEHVAIRHMLNLESVITYEGTQTIHQLSV
                     GRAITGISAF"
     gene            5258..5974
                     /locus_tag="VNZ06_15065"
     CDS             5258..5974
                     /locus_tag="VNZ06_15065"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWW22119.1"
                     /translation="MDRQQLRIAGLTGVEITLDIAGAGSRSLAFIIDWHIRLLGALAW
                     FLLVSAIGISTHLNAATRSVAAIVIVPALAIYLLYHPILEIFTRGTPGKRMAGVRIVT
                     RQGGTPSMGAMLIRNVFRLIDSLPFLYVAGLVCCLFSAQHVRIGDMAAGTLLILSDVE
                     SEASLGRLSAQLTGSKLAPNVIEIVNELLRRWHLLDTIRRDKVARLVLGSADTSLTAE
                     ALAPLGDAELYRRLKALYGT"
     gene            5964..6998
                     /locus_tag="VNZ06_15070"
     CDS             5964..6998
                     /locus_tag="VNZ06_15070"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HWW22120.1"
                     /translation="MEPEAQAAVWMKSRAAVWSQWIAQSQSPSRQLTVEQAHHRVERY
                     RAIARDLTIARKLAPRSAATTALESLYRSSHLRIDAAARHSVSSLLRLFGDEIPEVIA
                     TLRPTILWLALLMLVGALTGGWLISTYPDLISLIASQEMINKVQHGELWTDNLLSVTP
                     PALLSVTILSNNIFVSITAFCAGVLFGLGAFYIVAFNGLMLGAMFAFTHQYGMDGALL
                     RFVSAHGLVELSVICVAGAAGVALGESLIRPTLPTRLESFQFAASRVGRVLLACAILL
                     IGCGLIEGFISPNPGVPFLSRIVIGICWWVLMLLFLSGRLFRQRQAKSVVADASSNTH
                     SIPPGPIAQV"
     gene            complement(6928..8229)
                     /locus_tag="VNZ06_15075"
     CDS             complement(6928..8229)
                     /locus_tag="VNZ06_15075"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWW22121.1"
                     /translation="MALRQNALILLLLTGLVAIAGDWSGEFELARFWYLPAALLLLGL
                     AYERWMASRAAPELQINAPPRWFLGRATAIHFLIGHRLARALTLELAPGGAPEISMDR
                     SIRTISAAAERSTASAAVATARRLGIYPWPAMRIRIGGVLGLAWWPQVLKAAGGIRVV
                     PEILREPDRMPGIGRLAGLEGQQRMGSGAEVQQLREYQQGDPPRAIDWKASARSRRLI
                     SRDFSEDQQLDIVLAVDAGRASALAAGDTDRLALYANVAARLAQRAASLDDRVGLVVY
                     AERPLAALAPSHGEAAVTRVRAVLSDMSVQPVDSNPALAAVRIRSLAHQRCLVVLLTD
                     LDDASMTGELRTALRLLLPKHLPFVAGVASPRVQALGSARATYELDVYRSLAAQEYSN
                     TLRQNVMSLRALGAAAVLATPERLDRAVLNAYWTMRQRRRI"
     gene            complement(8240..9199)
                     /locus_tag="VNZ06_15080"
     CDS             complement(8240..9199)
                     /locus_tag="VNZ06_15080"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWW22122.1"
                     /translation="MSTPLQDLEQALQRSLNATVIGLEGVIHSIVIAIVARGHVLIQG
                     APGLGKTLLSKTLAAALGGSFKRVQGTADLMPSDIIGVHVFDNTRNEFSFRPGPLFTE
                     VLLFDEINRAGPKTQSALLEAMEERQVSVERKLYRLPTNFLVVATQNPRDFEGTYPLP
                     ESQLDRFMLRIDLSYPTHEHELQVLNRYGTIVAEPATATGSVHAPVIITPELLLAARQ
                     AADAIHVAPELAEYVLSLAKASREHTALTLGLSTRGALALLRAARIEAGLRGGQFVTP
                     DDVKAVVPQVLPHRLMLSSEALLEGSAESDIARRLLEQVAVPR"
     gene            complement(9196..10461)
                     /locus_tag="VNZ06_15085"
     CDS             complement(9196..10461)
                     /locus_tag="VNZ06_15085"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HWW22123.1"
                     /translation="MKQRLMTLLLALGALLLFYDFLLPKPPPDFVKPSLPLSTDNGAD
                     GEQAIWRWLTAEAVPVTSLRQRYAQLNRAASSNTGNVLITVMPHRTLIRNDEWAPLMS
                     WIGAGNTALVLAALDDTPNWSMGYPAGFGNEPGFLPELLRLTQLGFATVPQEKEKPRR
                     SELTQLLSKTDVTLEPRGVHPLMAQVHSLHASSDMPSARWLATPEGFHMPLALMQRTD
                     SQDPALWVSPHGHGQLIVLSVASAFANHEIDHADNARLLANIIGWSRAPRGQVVFDDA
                     HQGLSDEYDAHAFFADPRLHHTLWWIVLLWLAFVLGPLPLRSTFSPWCPVDETVLLDA
                     SGRFYSNAVTREDAARRLFENFFNGLRRRLSLTENGQPLWAWLVTQAAVPNAQRALLQ
                     GLYARMCAEQRVDLTQLQNLLTDLQGKLA"
     gene            complement(10458..>10783)
                     /locus_tag="VNZ06_15090"
     CDS             complement(10458..>10783)
                     /locus_tag="VNZ06_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22124.1"
                     /translation="DLPGLATLDATSLSERPALLLRLLVQALERSGRLAGDRTLTHRE
                     LIARAGFDGEGQRQRFASISLRAEQQVYGRAAAFVNDDPELQRVLHEGRELYSQLLAA
                     SSAAS"
BASE COUNT         1998 a         3391 c         3398 g         1996 t
ORIGIN      
        1 acgcagaccc acctgatgga tgaactcaag aaggtcaaac gtgtgatcgc gcgggtgatg
       61 cccgacgcgc cgcacgaagt gttgctgata ctggatgcga atcagggaca gaatgcgctg
      121 gcgcaggcac agcagtttca cgaagccctg ggggtgaccg gcctggtgct caccaaactc
      181 gatggcaccg ccaagggcgg gatcgtcgtg gcgatcgccc gttcgctgag aattccgatc
      241 cgcttcatcg gcatcggcga gaagtccgaa gattttggag tgttcaacgc cagcgccttt
      301 gccagcgccc tggtcgatgg ttcggaggcc ggcgcttcgt gatccgcttc gagcgcgtca
      361 gcaagcgtta ccccaacggg cgcgaagccc tctgcgaggt gagcttcgaa atcgccgatg
      421 gcgaaatggt gtttctgacc ggccgttccg gcgccggcaa gagcaccgtc ctgaaactga
      481 tcgcgttgct ggagcgtccc tcgcgtggcc aggtgatcgt caacggtgac agcaccgcga
      541 gactgccgtt gcgacgcgta ccgatgtatc gccggcgcat cggcgtcgta ttccaggagc
      601 atcgtctgtt gatggatcgg ccggtattcg acaatgtcgc gttaccgctg gtggtcgcag
      661 acacatcgtt tcgcgaaatg gaaaaacgcg tgcgcgccgc actcgaccag gttggcttgc
      721 tcggcaagga gcgcatggca ccgcaggagc tgtcggtggg cgaacagcag cgtgtcggca
      781 tcgcgcgcgc gatcatcagc aagccgccgc tgctggtagc ggatgagcca accggcaatc
      841 tcgatgccac gttggcggcg gaggtgatgg ggctgttcaa gcgcttcaac gaagtcgggg
      901 tcactgtgat cgtcgcgacc cacgacctgc acctggtaca ggaatcgggt aagcgcgaga
      961 tcgtactcga cgagggccga gtgctttgac gatggccaat ccactggcca attgggtctc
     1021 acgccacgga cacgcactct tttccagtgc cggacacctg gtgcgtcacg gtgtacccac
     1081 gttgctcacg gtcggtgtga tgggtctggc attgtcgctt cccctggcgc tgcaggtgct
     1141 cgtcagcaac gtgcgcgatg cgaccggcga tttttccggg gcagtgggca tgtcgatcta
     1201 cttcaagacc gacgtcccac cgcagaaggt acagcagctc gccaagaccg cggctgatcg
     1261 agccggtgtc gccagtgtga cgctggtcac ggcggcgcag gccctggaag aatttcgcac
     1321 tcagtccgga ttcggcgcag cgctggatgc gctcactgac aacccactgc ccaacgtgct
     1381 gacgatccgg cccacgccct tcagttcaac ccctgagaga ctgaccgcgt tgcgcgatta
     1441 ttttggcagc tggccggaag tggactcggt gcagctcgac agcgactggg cgctgcgctt
     1501 cggtgcgatc ctggaattgc tgcgcaggac ggtgctgatc gccgccgcgc tgctaggaac
     1561 cggtgtggtg gcagtcgtcg gcaacaccat ccgactcgag atcctcaatc gccgctccga
     1621 gatcgaggtc accaagctgg tgggcggcac gaatgcgttc gtacgccggc cgttcctgta
     1681 cgccggagct ctgtatggca tcgccgccgg cgcgatggcg tgggccatcg tcggcttggc
     1741 acgcatgtgg ctcgcgccgg ccgcagcgca tctggcttcc gcctacggca gccacttcgc
     1801 gctgcgcgga ccggacgcac gcgagttgca agtgctgttg ggcgcgggcg tggcgctggg
     1861 atgggtgggt gcctggattg ccgccgcgcg gcaactggcg cgcattgagc cgcgtgcgag
     1921 ctgaagagtt ggcgggccct tggattgcgt aaaggcgacc ccatgcatgg ggaattgcgt
     1981 cgcggctgcc agcctgatcg agctgccgat gatgatcggc tccccagggg agccgataaa
     2041 ccattgacag ggatgcataa atatatggcc cgcaggccct tagcactctg gcgatcagag
     2101 tgctaaaatt acgtccggaa ctggaggtgc gtgaacccat gagtgctcaa actgcgctgg
     2161 ttgcccatag ccccgggctg gctggtttgg ctctggccgg tccgatcggg agttttgacg
     2221 cctacctgga aagggtgggc cggattcctg tgctcacgcg agatgcggag caggagctcg
     2281 ctcagcggtt tcgccgcgac aatgacctgg atgcggcgcg ccagctggtg ctctcgcacc
     2341 tgcgatttgt cgttcacatc gcccgtggct atcagggcta cggactgccg atgaacgacc
     2401 tggtacagga aggaaacatc ggcctgatga aagccgtcaa acgcttcgac ccgagcgtag
     2461 gcgtgcgtct ggtgtctttc gccgtgcact ggatccgcgc cgagatccat gagtacgtgc
     2521 tgcgcaactg gcggttggtg aaggtggcca cgaccaaggc acagcgcaag ctgttcttca
     2581 acctgcggcg catgaagaag aacctgactt ggctgagcgc cgcggaaacc aaggccgtgg
     2641 ctcaggacct tggggtctcg agcgcggaag tgtccgagat ggagcgccgt ctgtccgccc
     2701 gggatctgtc atttgatgtg acggccgaat ccgatgaaga cgacggctac ggccctgcct
     2761 cgtatctgcc ggcggcggac gccgatccag cggaacaggt ggaagacgaa gagtggagca
     2821 gctcgaacca cgaccagctg gccgcggcca tgcaacggct ggacgagcgc agccggtcga
     2881 ttctccaacg tcgctggatg aacgacgaca aggccactct gcacgagctg gcggacgagt
     2941 acggcgtgtc ggccgaacgt atccgtcaga tcgaggcggg agcgatcgcc aagctcaagg
     3001 ggctgctacc cgcacccgtg taaggccggt ccttggttgc tatttgacgg cgcgcgcgaa
     3061 ccagatttcc acgcgccggt tttcgatcct gccctcgggc gtcccattgc tggcgagcgc
     3121 atcgttggct ccagcgccac gctgctgcag ccgcgcagcg gaaattccct gtgccgtgaa
     3181 gcagtcatac agcgccttgg cgcgggcagc agaaaaactc tggttggcac tttcaccgcc
     3241 gaggctatcc gtgtacaccg agatttgcgc ccccagcatt cgtcggcgtt tcagcagccg
     3301 cagcgccgca ctcactggca acgcgcgacg ggcatcggcc ttgagcgccg tgccgtccgc
     3361 ctcgaagacc cacgaggtgg agaacctcag cgtgaggtgg tcatctgctc gcagtatcgc
     3421 cactgcgctg ttgggcggca ggtccatcag ccagtcgtgc agcaggcttt cggcatgatc
     3481 cagctcgcgc tgttgcgcac gactgacctg gccgtgcgca atccgtggcg ccatcgtaca
     3541 taacgcaatg accaccacta tccagtaccg acatcgctcg ggcatcattt gcgcaattct
     3601 atgagatgag gccgccccat gagcacttcg accaagcccg cccccgctgc agccgccgct
     3661 gcagttccta ccgaaagcgc cgttgactat ctgcgcctgg acgaggaact caccgaagac
     3721 gaacgcttgg tccgcgattc cgtcgcgcgc ctggtcgacc agcgcgtgct gccgatcatc
     3781 ggcgaatgct tcgagaacag ccgttttccg aaagagctgc tgccctcgat tgccgaactc
     3841 ggactgctcg gctcgagtct ggagggctac ggctgcgccg gcctgaatgc ggtgtgctac
     3901 ggcctgatct gccgcgagct ggagcgcggc gacagcgcgc tgcgcagctt cgtctcggtg
     3961 cagtcgagcc tgtgcatgta cccgattcat gcctacggca gcgaggtcca gaagcagaaa
     4021 tggctgccgc gcatggcaaa gggcgaagtc gtggcctgtt ttggcctgac tgaggcgcac
     4081 ggcggctcgg atccggccaa catgaagacc cacgccaagc gctcggccga tggctgggtg
     4141 ctgaatggct cgaagatgtg gatcaccaac ggctacattg ccgatctcgc cgtggtctgg
     4201 gcccgcacgc ccgacggcgt gcgaggcttt ctggtggagc gcggcacacg cggcttcacg
     4261 tcggaggaga tcaagcacaa gttcagtctg cgtgcatcgg ccacgggcgc cctgtacttc
     4321 gatgatgtgc acctgccagc cgatgcgatg ctgcccgccg gcatcggcat caaagccccg
     4381 ctatcctgcc tgtcgcacgc gcgtttcggc atctgctggg gtgcgatcgg cgcagcgcag
     4441 gcctgcatcg ccgaggcgct gggtcatgtc ggcgaacgcg aactgttcgg ccgcacgctc
     4501 aaccacaatc aggcggtgca gatcagactg gccgagatgg tgcgtcagct ggccttaggt
     4561 caactgctgg caatgcaact ggcgcgcctg aaggaccgcg gggaactgac cccgacggcg
     4621 gtttcgctgg caaagtggaa caactgtcgc atggcgctgg agatcgcgcg tgactgccgc
     4681 gatctgctcg gtggcgcggg tatcagcagc gagcatgtgg cgatccggca catgcttaac
     4741 ctggaaagcg tgatcaccta cgagggtaca cagaccatcc accagctctc cgtcggtcgg
     4801 gcaattacgg gaatctcagc gttctagcac cgaccggagt gtgattgtcg ccacatcggc
     4861 gacattaagt catctgatta gcgtcgccga tccgcgacag aatgacaata aaatccctgc
     4921 gacattatgg tgttgccgca gctttggaga tattatggcg ttcagttgcc gcgattcgaa
     4981 agattgcggc aaaaatcgga cagccagtgt ttctgacgga tttcgagcaa gaaactcagg
     5041 cggtcggggt ggacacagac cgcggtgtgg ccgttttgaa cccaccgcaa gtggtcaaag
     5101 cgcgaactga cgcgcaggtc ccgcactttc gggactggcc cattggccgg acgctaaggt
     5161 ctgcatcgcg ttgagcgttc gcccgtgaca gacctgtgtc gtttgaggaa attatagaac
     5221 cccgttattt gcggggtttt ttttggataa tggcggcgtg gatcgccaac agctgcgaat
     5281 cgccggacta accggtgttg aaatcacact cgacatagcc ggtgccggta gtcgcagttt
     5341 ggcgttcatc atcgattggc acatccgcct gctcggcgcg ctcgcctggt ttctgctcgt
     5401 ctcggcgatc ggcatctcca cgcacctgaa cgccgcaacc aggtctgtgg ccgccatcgt
     5461 gatcgtgccc gcgctggcaa tctacctcct gtatcacccg atcctggaga tcttcactcg
     5521 cggtacgcct ggcaaacgca tggccggtgt gcggatcgtg acgcgccaag gcggaacccc
     5581 gtcgatgggc gcgatgctga ttcggaacgt gtttcgactg attgattcgc taccattcct
     5641 ttacgtcgcg ggactcgtct gctgcctttt cagcgcgcaa cacgtgcgca tcggcgacat
     5701 ggccgccggc acactgctga tattgagcga cgtcgaatcc gaggcatcgc tcggcaggct
     5761 cagcgcacaa ctcaccggga gcaagctggc tccgaatgtc atcgaaatag tcaatgagtt
     5821 gctgcgacgc tggcacctgc tcgataccat ccggcgcgac aaggtcgcgc gactggtgct
     5881 gggcagcgcc gatacctcgt tgacggccga ggcattggca ccgctcggcg atgcggaact
     5941 gtaccggcgg ctcaaggcgt tgtatggaac ctgaggctca ggctgccgtc tggatgaagt
     6001 cgcgagcggc cgtctggtcg caatggatcg cgcagtcaca gtcgccctcg cgacagctga
     6061 ctgtggaaca ggcgcaccat cgcgttgagc gctatcgagc gatcgcgcgc gatttgacga
     6121 tcgcccgcaa gctcgcccca cgcagtgccg ccaccactgc gctcgaatcg ctctaccggt
     6181 cgtcccattt gcgcatcgac gccgccgcac gacacagcgt ttcgagcctg ttgcgcctgt
     6241 tcggcgatga aatccccgaa gtcatcgcaa cgctgcgccc gactattctt tggctggcgc
     6301 tgctgatgct ggtcggagcc ctgaccggcg gatggttgat ctcgacctat ccggacctga
     6361 tcagcctgat tgccagtcag gaaatgatca acaaggttca gcacggtgag ttatggaccg
     6421 acaatctgct cagtgtgacg ccgcccgcgc tgctgtccgt cacaatcctg tcgaacaaca
     6481 tcttcgtcag catcactgcg ttctgcgccg gtgtgctgtt cggattgggc gccttctata
     6541 tcgtggcttt caacggcctg atgctcggcg cgatgttcgc cttcactcat cagtacggga
     6601 tggatggcgc actgctgagg ttcgtgagcg cacatggact ggtcgaactg tcggtgatct
     6661 gcgtcgccgg cgcggctggg gttgcattgg gagagtcgct gatcaggccg acgttgccca
     6721 cgcgactcga atcatttcaa tttgccgcca gccgcgtcgg tcgggtgttg ctggcctgcg
     6781 cgattctgct catcggctgc ggcctgatcg aaggcttcat ttcaccgaat ccgggcgtgc
     6841 cattcctctc gcgcatcgtc atcgggatat gctggtgggt gttgatgctg ctgttcctga
     6901 gcggccgctt gttccgtcag aggcaagcta aatccgtcgt cgctgacgca tcgtccaata
     6961 cgcattcaat accgcccggt ccaatcgctc aggtgtagcc agcaccgctg cggctccgag
     7021 tgcgcgcagc gacatgacgt tctggcgcag cgtgttgctg tactcctgcg ctgccaatga
     7081 gcggtagaca tcgagctcgt aagtcgcgcg tgcgctgccg agtgcctgca cacgagggct
     7141 ggcaacgcca gctacgaacg gcaggtgctt aggcaacaac aaccgcaggg cggtgcgcaa
     7201 ttctcctgtc atcgaggcat cgtccagatc ggtcagcaat accaccaggc agcgctgatg
     7261 tgccaatgag cggatacgca ctgccgccag cgccgggttc gaatccaccg gctgaactga
     7321 catgtcactc aacaccgccc gcacgcgcgt cactgcggcc tccccgtgac ttggcgccag
     7381 cgccgccagc gggcgctccg cgtacaccac gaggccgacc cggtcatcga gactggccgc
     7441 acgctgtgcc agccgcgcgg caacattggc gtacaaggcc aggcgatctg tgtcaccggc
     7501 ggccagagcg ctggcccgac cggcatccac agcgagcacg atatcgagtt gctggtcttc
     7561 ggagaaatcg cggctgatca ggcgtcgcga gcgagcgctg gccttccagt cgatcgcgcg
     7621 cggcggatcc ccctgctgat attcgcgcag ctgctgcact tcagcgccgg atcccatgcg
     7681 ctgctgcccc tcgagtccgg cgaggcggcc gatgcccggc atgcgatccg gctcgcgcag
     7741 aatctcgggt accacccgaa tcccaccggc tgccttcagc acttgtggcc accacgccag
     7801 gcccaggaca ccgccaatgc gaatccgcat cgcaggccat ggatagatgc ccagccggcg
     7861 tgccgtcgcc accgcggcgc tcgccgtgct gcgctcggcc gcagcgctga tcgtgcgtat
     7921 cgaacgatcc attgagattt ccggcgcacc acccggggcc agttccaacg tcagcgcgcg
     7981 tgcgagccga tgtccgatca gaaagtgaat ggcagtggcg cgacctagaa accagcgtgg
     8041 cggagcattg atttggagct caggcgccgc acggctcgcc atccaccttt cgtatgccag
     8101 ccccagcaac aatagcgccg ccggcagata ccagaatcgc gccaattcga attcaccgct
     8161 ccagtcgcca gcgatcgcaa ccaacccggt gagtagcagc agaatcagcg cattctgacg
     8221 taacgccatg gcggtctcgt cagcgcggca ccgcaacctg ctcgagcaat cgcctggcga
     8281 tgtccgattc ggcgctccct tcgagcaacg cctcgctcga taacatcagc cggtgcggca
     8341 acacctgcgg caccaccgcc ttcacgtcat ccggcgtgac gaattgcccg ccgcgcagcc
     8401 ccgcctcgat acgcgcggcg cgcagcaaag ccagcgcccc gcgggtcgac agtccaagag
     8461 tcagcgccgt atgttcgcgc gacgccttgg cgagactcag cacatactcc gccagctccg
     8521 gagcgacgtg gatcgcatcg gccgcctgcc gcgcggcgag cagcagctca ggcgtgatga
     8581 tcacgggcgc atgcacgctg ccagtcgcgg tcgccggctc tgcgacgatc gtgccgtagc
     8641 gattgagaac ctgtagttcg tgctcgtgtg tcggatacga cagatcaatg cgcaacatga
     8701 agcgatcgag ctgcgattcg ggcaacggat aggtgccttc gaagtcgcga ggattctgcg
     8761 tggcgaccac caggaagttg gtaggcagcc gatagagctt gcgctccacc gagacctgcc
     8821 gttcctccat ggcctccagc agcgccgatt gggtcttggg accggcgcga ttgatctcgt
     8881 cgaacagcaa cacttccgtg aacagcggcc ccgggcgaaa actgaattcg ttgcgggtat
     8941 tgtcgaacac gtgcacaccg atgatgtctg acggcatcag atcggcggtg ccctgtacgc
     9001 gtttgaagct gccaccgagt gccgccgcca gcgtcttgct cagcaaggtc ttgccgagac
     9061 ccggcgcacc ctgaatcagc acatgaccgc gcgcgacaat cgcgatgacg atcgagtgaa
     9121 tgacgccttc caggccgatc acggtcgcgt tgagcgagcg ctgcagcgcc tgctccagat
     9181 cctgcagagg cgtactcatg ccagttttcc ttgtaagtcg gtcaacagat tttgcagctg
     9241 ggtgagatcg acacgctgct cggcacacat ccgcgcatac aggccctgca gcagcgcgcg
     9301 ctgcgcattc ggaaccgccg cctgcgttac aagccacgcc cagagtggct gtccgttttc
     9361 cgtcagactc agccgtcgac gtagcccatt gaagaaattc tcgaacagcc gccgggccgc
     9421 atcctcccgt gtgacggcat tggaatagaa gcggccactg gcgtcgagca ggacggtttc
     9481 atccaccggg caccagggac tgaaggtact gcgcagcggc agcggaccga gcacgaacgc
     9541 caaccacaac agcacgatcc accaaagagt gtgatgcaaa cgcggatcgg caaagaaggc
     9601 atgggcatcg tattcatcgc tcaggccctg gtgagcgtcg tcgaagacaa cctgaccgcg
     9661 tggcgcgcgt gaccagccga taatattggc cagcaatcgc gcgttgtccg catgatcgat
     9721 ctcgtggttg gcgaacgcac tcgcgacgct caacacgatc agctgtccgt ggccatgagg
     9781 agagacccac aaagccgggt cctgactatc cgtgcgctgc atgagcgcca gcggcatgtg
     9841 aaatccctcg ggggtggcaa gccagcgggc cgatggcatg tcagagcttg catgcagact
     9901 gtgcacttgt gccatcaagg gatgcacgcc gcgtggctcg agcgtgacat cggtcttgct
     9961 cagcaactgt gtcagttccg accgtcgggg cttttccttc tcctggggta ccgtggcgaa
    10021 gccgagttga gtcagtctta gcaattccgg caggaacccg ggttcgtttc caaaccccgc
    10081 cgggtagccc atcgaccagt taggcgtatc gtccagtgcg gcaagcacca gagcggtatt
    10141 accggccccg atccagctca tcagcggcgc ccactcgtcg ttgcggatca gagtacggtg
    10201 aggcatcacc gtgatcaaca cattgccggt gttgctgctg gctgcgcgat tgagctgggc
    10261 atagcgctga cgcagactcg tgaccggaac tgcctcggcc gtgagccaac gccagatcgc
    10321 ctgttcaccg tcggcgccgt tgtcggtgct caagggcaac gacggcttga cgaaatcggg
    10381 tgggggcttc ggcagaagaa aatcatagaa cagcagcaga gcacccaacg ccagcagcag
    10441 cgtcatcagt cgctgcttca cgacgcggcg ctcgatgcgg ccagcagttg cgagtacaac
    10501 tcccggcctt cgtgcaggac gcgctggagt tcgggatcgt cgttcacgaa cgcggcggcg
    10561 cgcccgtaga cctgctgctc ggcacgcagt gaaatgcttg cgaaacgctg gcgctgaccc
    10621 tcgccgtcga accctgcccg cgcgatcagt tcccggtgcg tcaacgtacg atcgcccgcc
    10681 agccggccgc tgcgttccag cgcctgcacc agcagacgca gcagcagcgc aggacgctcg
    10741 gacaatgaag tcgcatccag ggtggcaagc ccgggcagat cgc
//
LOCUS       DATMRM010000495         2584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3693017, whole genome shotgun sequence.
ACCESSION   DATMRM010000495 DATMRM010000000
VERSION     DATMRM010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3693017"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VNZ06_15095"
     CDS             complement(<1..437)
                     /locus_tag="VNZ06_15095"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWW22125.1"
                     /translation="MARGLALENRFLDTMRTFGVAIVGCGMIARFHAKALLEVPQTRL
                     RALVSRSLANARAVAESVGLRCDIYTELTPVLARHDVDIVIVTTPSGAHMEPAVAAAQ
                     AKKHVVVEKPLEITLDRCDRIIDACARNGVQLCTIFPSRFGDA"
     gene            complement(401..1006)
                     /gene="pgsA"
                     /locus_tag="VNZ06_15100"
     CDS             complement(401..1006)
                     /gene="pgsA"
                     /locus_tag="VNZ06_15100"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HWW22126.1"
                     /translation="MATVPPPTRPSVFNLPNQLTAARLVLAVVLFVFIAYSSWLSCML
                     VFAVAVVTDGLDGYLARKQGLTSTLGRNLDPLVDKVLIGGAYIFLLPQGYAEGWLQPW
                     MVTLIVARELIITSLRSFLENQGVSFGADWLGKIKMVLQCAALCSIFIALYAVTLEAS
                     GQWLQLVTLARSCLIWAMLVATALSGLQYLWRAASLLKIDF"
     gene            complement(1009..2364)
                     /gene="rimO"
                     /locus_tag="VNZ06_15105"
     CDS             complement(1009..2364)
                     /gene="rimO"
                     /locus_tag="VNZ06_15105"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HWW22127.1"
                     /translation="MDAPKGTYAFVSLGCPKNLVDSERMLGRLAQEGYALVPDADGAD
                     VVVVNTCGFIEPARQESLGVIREMLALKQQGRVGAVVVAGCLAERKKDDLLAEVPEVD
                     HIVGVFGREEITQVVDRVLSQRLEQRSLFRPAPVRAQEDTARLRITPRHYAYLKISEG
                     CDRLCTFCAIPGMRGKHVTKPIEEVLREARELAADGVRELNIVAQDTTYYGMDLYGRV
                     RLAELLRELDRVDGLEWIRILYAYPIHFTDDLIETLAGADKILPYLDLPLQHINDRML
                     KRMQRKVNREETEQLLGKLRAALPGLTLRTTFIVGFPGESDAEFEELLAFARAQRFER
                     VGVFPYSLEPGTPAARLPDHLPEEVKLQRRNRLMEAQQEIAFAWTRQQIGRELEVLVD
                     GPDPEMPNHALARSHADAPEIDALVRVKSKRLHAGDLVRVKVTAADGYDLVARTIAPA
                     R"
BASE COUNT          503 a          799 c          818 g          464 t
ORIGIN      
        1 ttggcgtcgc cgaagcgcga cgggaagatc gtgcacagct gaactccgtt gcgggcacag
       61 gcgtcgatga tgcggtcgca gcggtcaaga gtaatctcga gcggtttctc caccacgacg
      121 tgcttcttcg cctgtgccgc cgccacggcc ggctccatgt gagctccgct gggagtggtg
      181 acgatcacga tatccacgtc gtgccgggcc aggactggtg tgagctctgt ataaatgtcg
      241 cagcgaagtc caacgctctc cgcgactgcc ctggcgtttg cgagactgcg gctgaccaga
      301 gcccgcaggc gcgtttgcgg cacctcgagc agcgccttcg cgtgaaagcg agcgatcatc
      361 ccgcaaccaa cgatggccac accaaacgtg cgcatggtgt ctagaaatcg attttcaaga
      421 gcgaggccgc gcgccacaga tactgcaagc cgctcaacgc cgtggcgacc agcatggccc
      481 agatgaggca gcttctggca agcgtcacga gctgcagcca ctggcccgaa gcttccagcg
      541 tgacggcgta cagggcaatg aagatactac aaagggcagc gcattgcagg accattttga
      601 tcttgccgag ccaatcggcg ccgaagctga ccccctggtt ctcgaggaag ctgcgcaggc
      661 tcgtgataat gagctcgcgc gccacgatca gtgtcaccat ccagggctgc agccagcctt
      721 ctgcgtaacc ctgcggcagc aagaagatgt aggcgccgcc gatcagcacc ttgtcgacca
      781 gcggatcgag atttcgcccc agtgtgctcg tcaacccttg cttgcgcgcc aggtagccgt
      841 cgaggccgtc cgtcaccaca gccaccgcaa aaacgagcat gcacgatagc caactgctgt
      901 aggcgatgaa cacgaacagg acgaccgcca gcaccagacg ggcggccgtc agctggttcg
      961 gcaggttaaa caccgaaggc cgggtgggag gcgggactgt cgccatgctc acctcgctgg
     1021 ggcaatggtc ctggcaacca gatcgtaacc gtcggcggcg gttaccttga cgcgcacgag
     1081 gtcgcccgcg tgcagccgct tgctcttgac gcgcacgagg gcgtcgatct ccggggcgtc
     1141 ggcgtggctg cgcgcgagtg catgattggg catttccggg tcgggaccgt cgacgagcac
     1201 ctccagctca cggccgatct gttgccgggt ccaggcgaag gcaatttcct gctgcgcttc
     1261 catgagccga ttacgtctct gcagcttgac ttcctcgggc agatgatccg gcaaccgcgc
     1321 cgcgggtgtg cccggttcga gcgagtaggg gaagacgccg acccgctcga agcgctgtgc
     1381 cctggcgaac gccagcaact cttcaaactc cgcgtcgctt tcgcccggga agccgacgat
     1441 gaaggtcgta cgcaatgtca ggcccggcag cgcggcgcgc agcttgccca gcaattgctc
     1501 ggtctcctcg cggttgacct tgcgttgcat gcgtttgagc atgcggtcgt tgatatgctg
     1561 caagggaaga tcgagatacg gcaagatctt gtcggcgccg gccagcgtct caatgaggtc
     1621 atcagtgaag tgaatcggat aggcgtagag gatgcgaatc cactctaagc cgtcgacgcg
     1681 gtcgagttcg cgcagcagct cggcgagccg aacgcggcca tagagatcca tgccgtaata
     1741 ggtcgtgtcc tgagccacga tattgagttc gcgcacgccg tccgcggcga gttcgcgcgc
     1801 ttcgcgaagc acttcttcta tgggcttggt gacatgcttg ccacgcatgc ccgggatcgc
     1861 gcagaaggta cacagccggt cgcagccctc tgagattttc agatacgcat agtgccgcgg
     1921 cgtgatccgc agccgggccg tatcttcctg cgcccgcacg ggagcgggcc gaaacagact
     1981 gcgctgttcc aagcgttgcg agagcacgcg gtcgaccacc tgcgtgattt cctcgcgacc
     2041 gaagacgccg acgatgtgat cgacttccgg cacctccgcg agcaggtcgt ccttcttgcg
     2101 ctcggccaga cacccggcga ccacgacggc gccgacacgt ccctgttgct tgagcgccag
     2161 catctcgcga atgacgccga gcgattcctg ccgcgccggc tcgataaagc cacaggtgtt
     2221 caccaccacg acgtcggcgc cgtccgcatc cggcacgagc gcgtagccct cctgtgccag
     2281 ccggccgagc atgcgctcag aatcgacgag gtttttggga cagccgagac tgacgaaggc
     2341 gtaggttccc ttcggagcat ccaccggcgg agcgggtttg ggtgtcaggg gaagttgaat
     2401 gaccggcaaa gagagtggac tcccacggtg cgcaaaggga tggagacgat tcgaacacgc
     2461 gaaaagtgat tctagcggtt tttcagcgaa ggcgtcaagt aaggctcggg atgggtcatt
     2521 agtcattcgt cattgtcatt cccacagaag tggtctgagc ctgaagcgta agcgaagtct
     2581 tcac
//
LOCUS       DATMRM010000496         9096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2466303, whole genome shotgun sequence.
ACCESSION   DATMRM010000496 DATMRM010000000
VERSION     DATMRM010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2466303"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..884)
                     /locus_tag="VNZ06_15110"
     CDS             complement(15..884)
                     /locus_tag="VNZ06_15110"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWW22128.1"
                     /translation="MLDYQKLKSLDLGIATHSYTQRDTMLYALGIGLGVDPLDASQLP
                     FVYEKNLRAVPSMVAILGGPGTAWRVEGMGVDWVKLVHGEQRIRLFRPLPAAATLTAT
                     NKVVSLTDKGAGKGAVMTIERVIYEKGNPEPLATTTQIAFLRGDGGFSAVSGVSDPGP
                     ESLPAVPDSKPDIELELPSLPQSALIYRLSGDYNPLHCDPEVARAAGFSRPILHGLCT
                     FGMAAHAVLNACCGYDASRIRSMAMRFTAPVYPGETVAFQIWRASADRLHLRARIVGR
                     DAVVLNGGLIELN"
     gene            1118..1498
                     /locus_tag="VNZ06_15115"
     CDS             1118..1498
                     /locus_tag="VNZ06_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22129.1"
                     /translation="MKNRKASASSEPGPRLQHVNQYLEKNFPDFFAEARFHVGSDDYF
                     LYSRFGQYLARSIESNCASPEKIRRAFTVLNKMARVSAKDPAVRRMLVTGPLEQIIDH
                     PKARALARKRLSTVAQGYLEGLCE"
     gene            1573..2202
                     /locus_tag="VNZ06_15120"
     CDS             1573..2202
                     /locus_tag="VNZ06_15120"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HWW22130.1"
                     /translation="MGSPSAFEILNFWFGPRPYPLAQVQQHMRLWFGEPDAPELTPQT
                     DELIRERFGELTRAAAAGSLSHWEAGPRRRLALILLLDQFPRNIFRGTARAFAQDRLA
                     LSLTLTGMQLGADATLDPVERIFFYMPLVHAEDPDVQEEGVAAFRRLRTEAPSEMCEI
                     FEASLASALEHRDLIARFGRFPHRNRSLGRETTADESSWLAAHSKSFGQ"
     gene            complement(2219..3340)
                     /locus_tag="VNZ06_15125"
     CDS             complement(2219..3340)
                     /locus_tag="VNZ06_15125"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005085.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HWW22131.1"
                     /translation="MSAEPPFRADHVGSFLRPKFLLEARDAAKQGTLSASELRSAEDR
                     AIRDIVSFQQDLGLSGITDGEFRRTYFHVDFLTQLDGVIEKGGTSVKFHKADGEIDYA
                     PPVMQVSGRVRHSKPIQRPDFEFLKSVTTRTPKVTIPSPTMLHFRGGRGAISVEAYPQ
                     LDAFYTDVANAYADEINDLASAGCRYLQLDDTNLAYLCDARQREAARSRGDDPDELPL
                     RYARLINAAIARRPPEMKVCVHLCRGNFRSSWVAEGGYEPVARVLFNELKVDGYFLEY
                     DDARSGDFAPLRHVPRGKRVVLGLVTTKLNKLETEDELRRRIDEAAQFLPLEQMCLSP
                     QCGFSSTVHGNDLAVEMQGRKLKLVVDTARAVWGDLQPR"
     gene            complement(3350..4684)
                     /locus_tag="VNZ06_15130"
     CDS             complement(3350..4684)
                     /locus_tag="VNZ06_15130"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWW22132.1"
                     /translation="MKVPAHQGCTLPNGVKLILIPRHDVPLIAMEAVVRGGASLDQPA
                     LAGVASLTAELLTRGAGERDAFAFAESVEDVGGSFEAEAHSEAVLLHGQFLARDRDRM
                     LGLLADALQRPHLAPDEFDTLRARRIEFIRAAKDSEPQSLIGAYGRSMLFAQHAYGAP
                     IGGSELTLAAVQRQHLLGFYRQQFGADRLTLVLAGDFEPEPMKQELAARFEHWQRASE
                     PLKPLDASLRVHGRRVLLIDAPGSTQTYFWIGNVGVARSYAARAALDVTNTAFGGSFG
                     SMLVQALRVKSGLTYSVSSGFRRGSVPGEFAISSFTQTESTASALSIALQTLSALKRD
                     GISAQGLNSARNYILGQYPLAFETAADWAGALAELDLYRLPDRYIDTYCDELAQVDLE
                     RSRQVIETAFPGAEDVDIVLIGDADCIRTGAAQFGPVTEKPLEAPDFEVAVS"
     gene            complement(4681..5946)
                     /locus_tag="VNZ06_15135"
     CDS             complement(4681..5946)
                     /locus_tag="VNZ06_15135"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWW22133.1"
                     /translation="MMQGQTSVQQLTLDNGLQVLLWPDQNIPNIALHHWVRVGSRNEG
                     PGSSGLAHFFEHMMFNGTLKHPQGDFDRIMESQGASNNAYTAQDVTVYQDWFPRAALD
                     QVFELESDRFAHLQFIPEVVENERKVVYSERRLRVEDSNAALLEEQVQATAFLAHPYR
                     IPIIGWPSDIQSWSLPALRHFYETWYAPNNRCLILTGAFEPDEVFALARKYFESAPSR
                     PLVPSAVTREPQQRGERRLVLERPGQNPLVQVAWHAIAASDPGQPALNLLQMILVVGD
                     ASRLHRSLVEEQELAVSIGGGWAEGFDPNIFSIYATLPSDGSAGAFESALDAQLARLL
                     EQGVSDRELERARNIAAMDFWRALSTIDGKARLLGEYAVMHDDYRLLFCAPQAYQRVT
                     PEQLLAVARRVFNCERRTVGVFKPVVAER"
     gene            complement(6235..6921)
                     /locus_tag="VNZ06_15140"
     CDS             complement(6235..6921)
                     /locus_tag="VNZ06_15140"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HWW22134.1"
                     /translation="MRNTGICLIVVVLGALVLSACSRESADWKSASTADTIEAYQQFL
                     TQHPKAANATAAQARLKALSEDHDWQVAAAADTRAAYEQFLGEHADSKWVQEARIRIE
                     NFAQSGSDSVTASSKPAVAAPAASSPASKKVASASVKAAPKKSGAVHANFVQLGAFSS
                     KERAQSQWETLAARFPTELKSLKPDYVPGKSKHGTVYRLRVPVSSVAAAKGLCGTLKK
                     HSQLCVPVTA"
     gene            6961..7374
                     /locus_tag="VNZ06_15145"
     CDS             6961..7374
                     /locus_tag="VNZ06_15145"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWW22135.1"
                     /translation="MSEIPRRRFLDRLSGSPSLLSAGSRLQGDVETAGPLMIAGHVIG
                     DGRVGGELSIGPDAHWEGNVYAQRAVVAGKVTGSIVVSEKIEISATAIIRGRVSASHI
                     AMALGATIDGDVAVTGSEPIVEFEDRRTADSTPTR"
     gene            7400..8155
                     /locus_tag="VNZ06_15150"
     CDS             7400..8155
                     /locus_tag="VNZ06_15150"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HWW22136.1"
                     /translation="MNRERLIHLVAHRGDAREFPENTLPAFTSALDLGVRFLELDVHL
                     SADGIPVVIHDHQLARTTGRRGLVFDLRAEELAQIEASEPQRFGDRYQGTRIPLLTDV
                     LALIKGRPEVTVFVEIKRASLTRFGHDQVVSRVVHTLRPAGAQCVVISFDLPAVHRAR
                     QLGGLAIGWVLTKYDDRSRMKYEALQPEFLFCDHLALPKGGALWRGPWRWAMYEVETL
                     ELAMSLAARGADYIETMAVREMSEAIRSARRGA"
     gene            8152..>9096
                     /locus_tag="VNZ06_15155"
     CDS             8152..>9096
                     /locus_tag="VNZ06_15155"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWW22137.1"
                     /translation="MSDGIDVDVVVVGAGIHGAGIAQAAAAAGHSVLLLEQTAPAAGS
                     SSRSSKLIHGGLRYLETGQFHLVRESLHERTTLMRLAPQLVRLQRFYIPVYRDTRRRP
                     WQLRIGLSLYAVLCGFGRGAGFGSLPRKQWDSLDGLETRDLQRVFYYHDAQTDDRALT
                     EAVVRSAQQLGVRMLCPAQVHRAALAQNLVDVYFGWDGSEQHCRARVLVNAAGPWADR
                     LARTISPHIAVPTLECVQGSHIILNSPSPGAIYYVESPRDGRAVFVMPWHEKLMIGTT
                     ETRFRGDPGAVAPTAAEVHYLLGVLKHYFPRFRSNDACE"
BASE COUNT         1647 a         2954 c         2818 g         1677 t
ORIGIN      
        1 cgcagaactg acgtctagtt cagctcgatc agcccgccgt tgagcacgac ggcatcacgc
       61 ccgacgatgc gcgcgcgcag atgcagccgg tccgcactgg cgcgccagat ctgaaatgcc
      121 accgtctcgc ccggatacac cggcgcggtg aaacgcatcg ccattgagcg gattctgctc
      181 gcgtcatagc cgcagcaggc gttgagcacg gcatgagcgg ccatgccgaa agtgcacagc
      241 ccgtgcagaa tcggacgcga aaagcccgct gcgcgggcga cctccgggtc gcagtgcagc
      301 gggttgtagt cgccgctcag gcggtagatg agcgccgatt gcggcaggga cggcaattcc
      361 agttcgatgt cgggcttgct gtcagggact gccggcaggc tttccgggcc cggatcgctg
      421 acgcctgata ctgcgctgaa gccgccgtcg ccgcgcagaa acgcgatctg cgtcgtggtt
      481 gccagcggct cagggttgcc cttttcgtaa atcacccgct caatcgtcat cacagcgccc
      541 ttgccggcac ccttgtcggt cagcgagacg accttgttgg ttgccgtgag cgtggctgcc
      601 gcgggcagcg gccggaacag tcggatgcgc tgctcgccgt gcaccagctt gacccagtcc
      661 acgcccatcc cctcgacccg ccaggcggtg ccagggccgc cgagtatcgc caccatcgat
      721 ggcacggcgc gcagattttt ctcgtagacg aatggcagct gcgaggcatc cagtggatcc
      781 acacctaagc cgatgcccag cgcgtagagc atggtgtcgc gctgcgtata ggagtgggtc
      841 gcgatcccga gatccagact tttgagcttt tggtagtcga gcatgaggct gatttcctgt
      901 cctgcacact gcagaacttc attctagtag aaccttctcg gcgccgtctg atcggacata
      961 agcaaaaact ttctgggttc ccgcactcaa ttcgcaacgc atcctattgc aaccaccgac
     1021 gatgtgtctt aacgttcgcg cgcgttgcga ggtgtggtac cgctcgtgac gtccaatgat
     1081 ggctgtctgg accaccggag gtgtgacaag aaaggcaatg aagaaccgca aagcgtccgc
     1141 atctagcgaa cctgggcccc gtcttcaaca cgttaaccag tacctcgaga agaattttcc
     1201 cgatttcttc gccgaagccc gtttccacgt cggcagcgac gactacttcc tctacagtcg
     1261 ctttggccaa tacctcgccc gctccattga gagcaactgt gcatcgccgg agaaaatccg
     1321 tcgggcgttc accgtcctca ataagatggc gcgcgtgtcc gccaaggatc cggccgtgcg
     1381 ccggatgctg gtcacgggac cgctggagca gatcattgat cacccgaaag cccgggcact
     1441 ggcccgcaag cgtctatcta cggtcgcgca gggctatctc gaaggcttgt gcgaatagaa
     1501 taagcgccgc ggcggcgggt tgacgatggg tccgctgcca cgcgtgcact agctgccggg
     1561 ggttcgctgc ctgtgggctc gccatccgcc ttcgagatcc ttaatttctg gttcggaccg
     1621 cgcccgtacc ccctggcgca ggtccagcag catatgcggc tgtggttcgg cgaacctgac
     1681 gctccggaac tcacgcccca gaccgacgag ctgattcgtg agcgcttcgg cgagctgacg
     1741 cgggcggcgg ccgccggcag tttgagccac tgggaagccg ggccacgccg acgactggcg
     1801 ctgatcctgc tgctcgatca gttcccgcgc aatatattcc gcggcacggc gcgcgcgttt
     1861 gcacaggacc ggctggcgct gtcgctcacg ctgaccggaa tgcagttggg tgcggacgcc
     1921 acgctcgacc cggtcgagcg catcttcttc tatatgcctc tggtgcatgc cgaggatccg
     1981 gacgtacagg aagagggtgt cgcggctttc cgtcgtctgc gcaccgaggc gccgtccgag
     2041 atgtgcgaga tcttcgaggc cagtctcgcc tccgcgctcg agcaccgtga cctcatcgcg
     2101 cgctttggcc gctttccgca tcgcaatcgc tcgctcgggc gtgagaccac cgccgatgag
     2161 tcctcctggc tggccgctca cagcaagagc ttcggccagt aacgccaccc cgacgtcgtc
     2221 agcgcggctg gagatcgccc cacaccgccc gagcggtgtc caccaccagc ttcagtttgc
     2281 ggccctgcat ctcgaccgcc aggtcgttgc cgtggacagt gctcgagaat ccgcattgcg
     2341 gtgacagaca catctgttcc agcggcagga actgagccgc ttcgtcgatg cgccggcgca
     2401 actcatcttc agtttccagc ttgttgagct tggtggtgac cagcccgagc acgactctct
     2461 tgccgcgcgg tacatgccgc agcggcgcga aatcccctga gcgagcgtcg tcgtactcga
     2521 gaaaataccc gtccactttg agttcgttga acagcacccg agccaccggc tcgtagccgc
     2581 cctccgccac ccacgaactg cgaaagttgc cacggcacag atgtacacat actttcatct
     2641 cgggcgggcg gcgcgcgatc gccgcgttga tgagccgtgc gtaacgcagc ggtagctcat
     2701 ccggatcgtc gccacgggaa cgcgccgcct cgcgctgccg ggcgtcgcat agataggcaa
     2761 ggttggtgtc gtcgagttgc agatagcggc aaccggccga cgcgagatca ttgatctcgt
     2821 cggcataggc attggccacg tcggtgtaga aggcatcgag ctgcggatag gcttcgacgc
     2881 tgatcgcacc gcgcccgccg cggaaatgca gcatcgtcgg cgaaggaatc gtcaccttcg
     2941 gcgtgcgggt agtgaccgac ttcaggaact cgaaatccgg gcgctggatc ggctttgagt
     3001 gccgtaccct gccggacacc tgcatgaccg gcggcgcata gtcgatctcg ccgtcggctt
     3061 tatgaaactt cacgctggta ccgcccttct cgatcacccc gtcgagctgg gtcaggaagt
     3121 ccacgtgaaa ataggtgcgt cggaactctc cgtcggtgat gcccgaaagc ccgagatcct
     3181 gctggaaact cacgatatcg cggatcgcgc gatcctccgc cgagcgcagc tcgctggctg
     3241 acagcgtgcc ttgcttcgcg gcatcgcgcg cctcgagcag aaacttaggc ctcaggaagc
     3301 tgccgacgtg atcggcgcgg aacggcggct cggcgctcat gcgcctactt tagcttacgg
     3361 cgacctcaaa gtccggcgcc tccaggggct tctcggtcac cggaccaaac tgcgcggccc
     3421 ccgttcggat gcagtccgca tctccgatca gcacgatgtc gacatcctcg gctcccggaa
     3481 acgccgtttc gatcacctgt ctcgagcgct ccaggtccac ctgagccaac tcgtcgcaat
     3541 aagtatcgat gtagcggtcc ggcaagcgat aaagatccag ttccgcgagt gctccagccc
     3601 agtccgcggc ggtctcgaag gccagcgggt actggcccag gatgtagttg cgcgccgagt
     3661 tcagaccctg cgcgctgatg ccatcgcgct tcagcgccga gagcgtctgc agtgcaatgg
     3721 acagcgcgga agcggtactt tcggtctgcg taaatgaact gatggcgaac tcgcccggca
     3781 cgctgccccg acgaaacccg gagctgaccg aataggtgag cccgctcttc acccgcaatg
     3841 cctggaccag catcgacccg aagctgccgc caaaagccgt gttggtcacg tccagtgccg
     3901 cacgcgctgc ataggagcgt gcgaccccaa cattgccaat ccagaagtag gtctgggtag
     3961 aacccggagc atcgatcaac agcacccgtc ggccgtggac gcgaagtgag gcatcgagcg
     4021 gcttcaaggg ttcggacgcc ctctgccagt gttcaaaccg ggctgcgagc tcctgcttca
     4081 tcggttcggg ctcgaagtcg ccggccagca ccagcgtcag gcggtccgcg ccgaactgct
     4141 ggcgatagaa accgagcaga tgttgccgct gcacggccgc cagggtgagc tcgctgccgc
     4201 cgatgggtgc cccataggca tgctgcgcaa acagcatcga acgtccgtag gcaccaatca
     4261 ggctttgcgg ctcggaatcc ttcgcggctc ggatgaattc gatccggcgc gcacgcaaag
     4321 tatcgaattc gtccggcgcg aggtgcggcc gctgcagcgc gtcggccaac aggcccagca
     4381 tccgatcgcg gtctctggcg aggaattgcc cgtgaagcag caccgcctcg ctatgcgcct
     4441 ccgcctcgaa actgccgccg acgtcctcca cgctttcggc gaacgcgaag gcatcgcgct
     4501 caccggcgcc acgcgtgagc agttcggctg tcagggaggc gacgcctgcg agcgcgggct
     4561 ggtccagact ggcgcctccc cgcaccaccg cctccatcgc gatcaacggc acgtcgtgcc
     4621 gcggaatcaa tatcagcttg acgccgttgg gcagcgtgca gccctgatgc gccggcactt
     4681 tcatcgctcg gccaccacgg gcttaaacac gcccaccgtc cggcgttcgc agttgaagac
     4741 gcgccgcgcc acggccagga gttgctcggg tgtcacgcgc tggtaggcct gcggcgcgca
     4801 gaacagcagc cggtagtcgt cgtgcatgac cgcgtattcg ccgagcagtc gcgctttgcc
     4861 gtcgatggtc gacaatgcgc gccagaaatc catcgccgcg atgttccgcg cccgctccag
     4921 ctccctgtcc gagaccccct gctcgagcag ccgcgccaac tgcgcatcca gcgcggactc
     4981 gaacgcgccc gccgatccat ccgacggcag cgtcgcatag atcgaaaaga tattcggatc
     5041 gaagccctcg gcccaaccac caccaatcga gaccgccagc tcctgctcct cgaccaggct
     5101 gcgatgcagg cgcgacgcgt ccccgacaac cagaatcatc tgcagcagat tcagcgccgg
     5161 ctggcccgga tccgacgccg cgatcgcgtg ccaggcgacc tggaccagcg gattttgccc
     5221 gggacgctcg agcaccaggc gccgctcacc acgctgctgc ggttcgcggg tgaccgcgga
     5281 gggcacaagc gggcgcgatg gggcggattc gaaatacttt cgcgcgagtg cgaagacctc
     5341 atccggctcg aacgcgcccg tgagaatcag gcagcgattg ttcggtgcgt accacgtctc
     5401 atagaaatga cgcagcgccg gcaggctcca ggactggata tccgacggcc agccgatgat
     5461 cggaatccga tacggatgtg cgagaaaagc cgtggcctgc acctgctctt cgagcagcgc
     5521 cgcattgctg tcctcgacac gcagacgtcg ctcggaatag accaccttgc gctcattctc
     5581 gaccacctcg ggaatgaact gcagatgagc aaagcggtcg ctttcgagtt cgaacacctg
     5641 atcgagcgct gcgcgtggga accagtcctg atacaccgtg acatcctgcg cggtgtaggc
     5701 attgttgctc gcgccctgcg actccatgat gcgatcgaag tcaccttgcg gatgcttcaa
     5761 cgtgccgttg aacatcatgt gctcaaagaa gtgcgccagt ccgctgcttc ccgggccctc
     5821 gttgcgactg cccacgcgca cccagtgatg gagcgctatg ttcggaatgt tctgatcggg
     5881 ccacagcaga acctgcagcc cgttgtccag cgtcagctgc tggacgctcg tctgcccttg
     5941 catcatgcct ctagcatagg ggcgagagat tgcgagaaaa ggccggccca aaagaaaagc
     6001 gccgcgaacc ggggaaagtg cgcggcgcca caggggcgaa catcggggaa cgttgttcgc
     6061 aaaggttagg ggaaaacctt acagctctat taaagcaagc tctatgccag cgcttgattt
     6121 gtttaaaaat caaatagata gcaattcagc ttaacctccg agcccccacg ggatgacaat
     6181 ttacgtcagt cgatgacgtg aatctgtctg taggatgacg cagcgcgcca gccttcaggc
     6241 cgtgaccgga acgcaaagct gcgagtgctt tttcagggta ccgcacaacc ccttggcggc
     6301 cgcgaccgag gagaccggaa cgcgcagccg gtacacggtt ccatgcttgg acttgcccgg
     6361 aacgtagtcc ggcttcagcg atttcagctc ggtcggaaag cgcgcagcga gcgtctccca
     6421 ctgcgactgt gcgcgctcct tgctgctgaa agcgcccaac tgcacgaagt tcgcgtgaac
     6481 ggcgcccgat ttcttcggcg cggctttgac cgaggcgctc gccactttct tggacgccgg
     6541 actgctggcg gcgggtgcgg cgacagccgg cttcgaagaa gccgtgactg agtcggaacc
     6601 gctctgcgcg aagttttcga tccggatgcg cgcctcctgc acccacttgc tgtcggcatg
     6661 ctcgccgagg aactgctcgt acgcagcgcg cgtgtccgcc gcggcagcga cctgccagtc
     6721 atggtcctcg gacagcgcct tcaggcgcgc ctgcgctgca gtggcgttcg cggccttggg
     6781 atgttgcgtt aaaaattgct ggtaggcctc aatggtgtcc gccgtcgagg ccgacttcca
     6841 gtcggcactc tcgcggctgc aggctgagag caccagagcc cccaggacta cgacgatcaa
     6901 gcagattcct gtgttcctca tcacatcctc cctggctcgc gaggcgaata attgtagacc
     6961 atgagcgaga taccgcgtcg acgcttcctc gaccgcctga gcggttcacc ctcgttgttg
     7021 tcggcaggct ctcgcctgca gggcgacgtc gagaccgcag gtccgctgat gattgcgggt
     7081 cacgtgattg gcgacggtcg cgtcggtggc gaactatcga ttggtccgga tgcgcattgg
     7141 gaaggcaacg tgtatgcgca gcgtgctgtg gtcgccggca aagtcaccgg cagtatcgtc
     7201 gtcagcgaga agatagaaat ctccgccacc gcgatcatcc gcggacgcgt ctcggcctct
     7261 cacatcgcca tggcgctcgg ggcgacgatc gatggtgatg tcgccgtgac cggctccgaa
     7321 cccatcgtcg agttcgaaga tcgccgtacc gccgacagca cgccgacgcg ctgaggtagc
     7381 ttgaggctat acttcggcgg tgaaccgaga acgattgatt catctggtcg cccatcgcgg
     7441 tgacgctcga gagtttcccg aaaacaccct tccggcattc acttccgcac ttgatctggg
     7501 tgtgcgtttc ctcgagctcg acgttcacct ctcggccgac ggcatcccgg tggtcattca
     7561 cgatcatcag ctggcgcgca ccaccgggcg gcgcggcctc gtgttcgatc tgcgagccga
     7621 ggaacttgcg caaatcgagg ccagcgagcc gcaacgcttc ggcgatcgct atcagggcac
     7681 acgcattccg ctgctcaccg atgtgctcgc gctcatcaag gggcgtcccg aggtgaccgt
     7741 gttcgtcgag atcaagcgcg cctcgctgac gcgcttcggc cacgatcagg tggtctcgcg
     7801 tgtggtccat acgctgcgcc cggcaggcgc acagtgcgtg gtgatttcat tcgatctgcc
     7861 agcggtgcac cgggcgcgcc agctcggcgg gcttgccatc ggctgggtgc tcaccaagta
     7921 tgacgaccgc agccgcatga aatacgaagc gttgcaacca gagtttctgt tctgcgatca
     7981 cctggcgcta cccaaggggg gagcgttgtg gcgcggcccg tggcgctggg ccatgtatga
     8041 agtcgagaca ctggaactcg ccatgtcgct cgccgcccgc ggcgcggatt acatcgagac
     8101 catggcggtg cgtgagatga gcgaagcgat tcgctcggcg cgccggggcg cgtgagcgac
     8161 ggcatcgacg ttgacgtggt ggtcgttggc gccgggatcc acggagccgg catcgcgcag
     8221 gccgccgccg cggccggtca ttcggtactg ctactcgagc agacggcccc cgcggcaggt
     8281 tcctccagtc gctcgagcaa attgattcac ggtggattgc gctatctgga aaccggccag
     8341 tttcatctcg tgcgcgagag cttgcacgag cgcacgacgc tgatgcgcct ggcaccacag
     8401 ctggtgcggt tgcagcgttt ctatattccc gtgtaccgcg ataccaggcg ccgcccatgg
     8461 cagcttcgaa tagggttgtc gctctatgcc gtgttgtgcg gcttcggccg gggtgccgga
     8521 ttcggctcgc tgccgcgaaa gcagtgggat tcgctggacg gtctggaaac gcgcgatctg
     8581 cagcgcgtgt tttactatca cgatgcgcag accgatgatc gcgcactcac cgaagcggtg
     8641 gtgcgctcag cgcagcagct gggcgtgcgg atgttgtgtc cggcgcaggt gcatcgcgct
     8701 gcgctcgccc agaatttggt cgatgtctac ttcggctggg atggcagcga acaacactgt
     8761 cgtgcacgcg tcctggtgaa cgccgccggg ccctgggccg accggctcgc gcgtaccatc
     8821 tcaccgcaca tcgcagtgcc gacgctcgag tgcgttcagg gctcgcacat cattctgaac
     8881 agccccagcc cgggcgctat ctactatgtg gaaagcccgc gtgatggccg cgcggtattc
     8941 gtcatgccct ggcatgaaaa actgatgatc ggaaccaccg agacgcgctt ccggggtgat
     9001 cccggcgcgg tcgcaccaac ggccgcggag gttcactacc tgctgggtgt gctgaagcat
     9061 tactttcctc gtttccgctc caacgacgcc tgtgaa
//
LOCUS       DATMRM010000497         5862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2160321, whole genome shotgun sequence.
ACCESSION   DATMRM010000497 DATMRM010000000
VERSION     DATMRM010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2160321"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VNZ06_15160"
     CDS             complement(<1..863)
                     /locus_tag="VNZ06_15160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWW22138.1"
                     /translation="MRTCDVIVVGGGPAGASAARRLRAGGADVLVLDKEVFPRLKLCA
                     GWITPDAVRDVNLDPLAYPQRFLTFRRLHFHIKGLRLPLPCIQHSIRRYEFDEWLLQQ
                     SGAPVLTHNVKEIRRDGSDYVLDGQFRCRYLIGAAGTSCPVHRALFRSALPRVRALQT
                     VTLELEFPYPWQDADCHLWFFEEGLPGYSWYVPKARGWLNVGVGGMAQRLKQRGEDIW
                     LHWQRFAQRLAPRFGITLPAEPTGYSYYVLGPVERVRIGNALLTGDAAGLATRDLCEG
                     IGPAIRSGLRAA"
     gene            complement(839..2131)
                     /locus_tag="VNZ06_15165"
     CDS             complement(839..2131)
                     /locus_tag="VNZ06_15165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HWW22139.1"
                     /translation="MNANPESEDFSDLDVQDDVPSVEASHVARPSLYGRLLRVLLRAI
                     GEPAIEFVLWTGEGVVASGAPIISRVHIRDRATLFGMLRDPFVRFGDAYCNGQIEVEG
                     DLIQTLSVISRVGQVREANPSILHRLARWVHRPSRNTFDGSRDNIHHHYDISNEFYAL
                     WLGETMAYTCAYYPTADTTLDQAQIAKMDHVCRKVRLNSGDSVVEAGCGWGSLAIHMA
                     SRYGARVRAFNISREQLSFARDRAKELGLADRVEFIEDDYRNITGRYDAFVSVGMLEH
                     VGPEHYPELGRIAHNAMKSEALGLIHSIGRNRPAPLQPWIERRIFPGAYPPALSEMSQ
                     IFEPWSFSVLDIENIRLHYALTLRDWLKRYRAAEERVRAMFDEKFVRMWRLYLAGSIA
                     GFETGTLQLFQVVFTSGQNNRVPMTREHLYSCAPATSS"
     gene            complement(2227..3288)
                     /locus_tag="VNZ06_15170"
     CDS             complement(2227..3288)
                     /locus_tag="VNZ06_15170"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWW22140.1"
                     /translation="MTLVPWYGIAHGYHAAAWIWFAVFVVLNEMSITCGYHRLLAHSA
                     YDAHPVLKGFYLLFGAMALQNSALIWSAGHRVHHRFIDDPQRDPYCAKKGFWFSHIGW
                     MLHNYESGAIDLDNVKDLQRDPLVMFQHRYYLAVALSANFGLPILVGLCTDDFWGTFL
                     VAGFLRLVVSHHATFFINSLAHMWGSQPYTDKNTARDNDVIAVLTFGEGYHNFHHMFA
                     HDYRNGVRWWQWDPSKWFIHLMSWVGLTRNLKRVPWFKIQRAMLDRQFQRVEVKLASH
                     PARAQLELIRSRIAEEYETFRKAVAEWAHLREQWIGDTKRAVVERWEKSTLQARLREL
                     EYGLQLQYRRMQVLQAQLR"
     gene            complement(3533..4450)
                     /locus_tag="VNZ06_15175"
     CDS             complement(3533..4450)
                     /locus_tag="VNZ06_15175"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HWW22141.1"
                     /translation="MPASYLLIVAAVLVLIALAKTLTVVPQQSAYVIERVGRYSRTLS
                     AGFHILLPGMDRIAYRHSLKEQALDVPEQVCITRDNVQVGVDAVLYMQVLDPHLASYG
                     ITNYGFAIAQLAQTTLRSEIGKIELDRTFEARGIINANVVAELDKASVAWGVKVMRYE
                     IKNITPPKDVLSAMEKQMRAEREKRAVILASEGERDAKINQAEGDKQRVIKASEANRQ
                     QQVNEAEGQASAILAVANATAEGLRQVGGALSERGGLDAMQLRIGEEYLRQFGKLAKD
                     STTLIVPGNLSDLASVVTMATSIVRKERQ"
     gene            complement(4474..4905)
                     /locus_tag="VNZ06_15180"
     CDS             complement(4474..4905)
                     /locus_tag="VNZ06_15180"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HWW22142.1"
                     /translation="MNWWVWIVGGAILLGSELGLINTEFYLLFVGGSALLTGVVALVY
                     PDFPAWAQWALFAVTCVVSTLGFRRHLYERLYRHSPKVATGPEGKELLLPSPLAPGGT
                     CQAEHGGSFWTVRNDSSAPLAAGCKVRIARVQGLTLLVRPE"
     gene            complement(4961..5746)
                     /locus_tag="VNZ06_15185"
     CDS             complement(4961..5746)
                     /locus_tag="VNZ06_15185"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWW22143.1"
                     /translation="MGLPIAQRWFEFERLSDDITRIWEPHALRLMQCNIWHVRGRDRD
                     LLIDTGFGVASLHEAARHLFDKSLTAVATHSHVDHVGSFHEFADRVIHKAEAADVAVE
                     GENYSLYSAEHPSEIIGSIRRAGYEVGPTFITALPHAGFNMSVFTRKAAPATRTVEEG
                     DVIDLGNRRFEVLHLPGHSPGSIGLWEAESATLFSGDAIYDGPLLDELPGSDIGVYVE
                     TMQRLQSLPARVVHAGHDPSFDGLRLRQLARAYLQRRQPIRLF"
BASE COUNT         1150 a         1883 c         1760 g         1069 t
ORIGIN      
        1 gcggcccgca ggccgctgcg gatggccgga ccgatgccct cgcacagatc gcgcgtggca
       61 agacccgcgg catccccggt gagcagcgcg ttgccgatgc gcacccgctc gaccggcccg
      121 agtacgtagt agctgtagcc ggtaggttct gccggcagtg tgattccgaa gcgcggcgcc
      181 aagcgttgcg cgaagcgctg ccagtgcagc cagatgtcct caccccgttg tttgagccgt
      241 tgcgccatgc caccgacccc gacattgagc cagccgcgcg ccttcgggac gtaccagcta
      301 taaccgggca gaccctcctc gaagaaccac agatgacaat ccgcgtcctg ccacggatac
      361 ggaaactcca gttcgagcgt gacggtttgc agcgcacgca cgcgcggcaa ggcgctgcga
      421 aacaacgcac ggtgtaccgg acaggatgtg cctgcggcac caatcaggta tcggcagcga
      481 aactgcccgt ccagtacgta gtcgctgccg tcacgtcgga tttccttgac gttgtgggtg
      541 agcactggcg cgccggattg ctgcagcagc cattcatcga attcgtaacg gcggatggaa
      601 tgttgtatgc acggtagcgg cagccgcagt cccttgatgt gaaagtgcag cctgcgaaac
      661 gtcaggaagc gctgcggata ggccaacgga tcaagattca catcccgcac ggcgtccgga
      721 gtgatccagc cggcacacag cttcagccgt ggaaagactt ccttgtcgag caccagcacg
      781 tcggccccgc ccgccctcag gcgcctcgcg gccgaggcgc ctgccgggcc gccaccgact
      841 acgatgacgt cgcaggtgcg cacgaataca agtgctcgcg cgtcatcggg acgcgattgt
      901 tttgtcctga ggtaaagacc acctgaaaca gctgcagggt gccggtttca aatcccgcga
      961 tcgatccggc cagatacagc cgccacatgc gcacgaactt ctcatcgaac atggcacgca
     1021 cgcgctcttc agcggcccga tagcgtttga gccagtcgcg caacgtgagc gcgtagtgca
     1081 gacgaatgtt ttcgatgtcg agcactgaaa aactccacgg ctcgaagatc tgcgacatct
     1141 cgctcagagc cggcgggtag gctccgggga agatgcgccg ctcgatccag ggctgcaacg
     1201 gcgccggccg gttgcggccg atcgaatgga tcagtccgag cgcctcgctc ttcatcgcat
     1261 tgtgtgcgat acggcccagt tccggatagt gctccgggcc gacgtgctcc agcatgccca
     1321 cggacacaaa cgcatcgtat cgtccggtga tgttgcggta gtcgtcctcg atgaattcca
     1381 cgcgatcggc cagaccgagc tccttggccc gatcgcgcgc aaagctcagc tgctcgcgtg
     1441 aaatattgaa tgcgcgcacc ctcgcgccgt agcggctggc catgtgaatc gccaagcttc
     1501 cccagccgca gccggcttcg accacgctgt cgccactgtt gagccgcacc ttgcgacaga
     1561 cgtgatccat tttcgcaatc tgcgcctgat cgagcgtcgt atccgccgtc ggatagtagg
     1621 cgcaggtgta ggccatggtt tcacccagcc acagggcata aaactcgttg ctgatgtcgt
     1681 agtggtgatg gatgttgtcg cgagagccgt cgaatgtgtt ccggctcggc cgatgcaccc
     1741 agcgcgccag ccgatgcaga atcgaagggt tggcttcgcg cacctggccg acacgggata
     1801 tcaccgacag ggtctggatg agatcgcctt cgacctcgat ctggccgttg cagtatgcat
     1861 cgccgaaccg cacgaacggg tcgcgcagca tgccgaacag cgtcgcccga tcccggatat
     1921 gcacccggga gatgatgggg gcgccggagg ccaccacacc ctcaccggtc cacagcacga
     1981 attcgatcgc cggctcgccg atggctcgca gcaatacgcg cagcaggcgt ccgtacaaag
     2041 aagggcgcgc aacgtgcgag gcttcgacgc tcggcacgtc gtcctggaca tctagatccg
     2101 agaaatcctc ggattcagga ttggcgttca ttcattcccc atgagagagc caaccgaaaa
     2161 cctgacgggt gccagcttac ttggtcgcga gctttttttt cggccgtcag tgcggccgga
     2221 caggcttcaa cgaagctggg cttgaagcac ctgcattcga cgatactgca actgtaggcc
     2281 gtattccagt tcacgtaggc gcgcctgcag ggttgacttc tcccagcgct ccacgacggc
     2341 tcttttggtg tccccaatcc actgttcgcg caggtgggcc cattcggcca ccgccttgcg
     2401 gaaagtctcg tattcctcgg cgatacgcga gcggatcagc tccagctgcg cgcgggccgg
     2461 atggctcgcg agcttgactt ccacccgctg gaactgccga tccagcatgg cgcgctggat
     2521 cttgaaccaa ggtacgcgct tgaggttgcg cgtcagaccc acccagctca tcagatgaat
     2581 gaaccacttc gacggatccc actgccacca acgcacccca ttgcgatagt cgtgcgcaaa
     2641 catgtggtga aagttgtggt aaccctcgcc aaatgtcagc accgcaatga cgtcattgtc
     2701 ccgagctgta ttcttgtccg tgtagggctg actgccccac atgtgggcca gcgaattgat
     2761 aaaaaaggtg gcgtgatggc tgacgaccaa ccgcagaaaa cccgcaacca ggaacgtccc
     2821 ccagaaatcg tcggtgcaaa gacccaccag gatcggaagc ccgaaattcg cactcagcgc
     2881 gaccgccaga tagtagcgat gctggaacat caccagcgga tcgcgctgca ggtctttaac
     2941 gttgtcgaga tcgatcgcgc cgctctcgta gttgtgcagc atccagccga tgtgcgagaa
     3001 ccagaaaccc ttcttggcgc aatacggatc gcgctgtgga tcgtcgatga agcggtgatg
     3061 cacgcgatga ccggcggacc agatcagggc gctgttctgc agagccatgg cgccgaacag
     3121 caggtagaag ccctttagaa ccggatgcgc gtcatacgcg ctgtgtgcca ggagtcggtg
     3181 atagccgcag gtaatggaca tctcgttgag gaccacaaac acggcaaacc agatccaggc
     3241 cgccgcgtgg tagccgtggg cgattccgta ccacggaacg agcgtcactg acaccaaagt
     3301 tgtcagtgaa aacataatca tagctggcca atttacaggg acagatgagg tactgttctc
     3361 cgacaccgcc agctctccta ctcgctgcga cactggggag tatcttatgc taatcgtttc
     3421 gggggtagca ggacttctac atactttctg acgccaacag gtcaccgatg gcgcagcaaa
     3481 ccgcgccgat tgctcaaatt gtgacgctga cccccgctgt cgcttttttc ccctactgtc
     3541 gttcttttcg cacaatgctg gtggccatgg tgaccaccga tgccagatcg ctcaggttgc
     3601 ctggcacgat cagggtggtg ctgtccttgg cgagcttccc gaactgtcgc aggtactcct
     3661 cgccgatccg cagttgcatc gcgtccagtc ctccccgctc cgacaaggct ccgccgacct
     3721 gccgcaagcc ctcggcggtc gcattggcca ccgcgagaat ggcggatgcc tgaccttcgg
     3781 cttcgttcac ctgctgctgc ctgttcgcct cagaggcctt gatgacccgc tgcttgtcgc
     3841 cctcggcctg gttgattttg gcgtcgcgtt caccttccga agccaggatc acggcacgct
     3901 tttcccgctc ggcacgcatc tgcttttcca tggcggacag cacatccttc ggcggcgtga
     3961 tgttcttgat ctcgtagcgc atcaccttga cgccccaggc caccgaggcc ttgtcgagtt
     4021 cggcgaccac gttggcgttg atgatcccgc gcgcctcgaa ggttcgatcc agctcgatct
     4081 tgccgatctc gctgcgcagc gtggtctggg cgagctgggc aatggcgaaa ccgtagttcg
     4141 tgatgccgta ggaggccagg tgcggatcca gcacctgcat gtacagcacc gcatcgacgc
     4201 ccacctgcac gttgtcgcgt gtgatgcaga cctgttcggg cacgtcgagc gcctgctcct
     4261 tcagggaatg gcgatacgcg atgcggtcca tgcccggcag gaggatatga aacccggccg
     4321 aaagcgtgcg gctataacga ccaacccgtt cgatgacgta ggcgctctgc tgcggcacga
     4381 cggtgagcgt tttggccaac gcaatcagga ccaagacggc ggcaacgatc aaaagataag
     4441 aagcaggcat agtgaatttc cagactgggt tggttactca ggccgcacca gaagcgtcag
     4501 cccctgaacg cgcgcgatcc gtaccttgca cccggccgcc aggggcgccg agctatcgtt
     4561 tcggaccgtc cagaagctgc cgccatgttc agcctgacag gtaccgcccg gtgcgagcgg
     4621 actgggcagc agcagctctt tgccctcggg tccggtggcg actttgggcg aatgccggta
     4681 cagacgctcg tacaggtggc gacggaaacc cagcgttgag acgacgcagg tcactgcaaa
     4741 cagcgcccac tgtgcccagg ccggaaagtc gggatagacg agtgccacta ctccggtcag
     4801 cagcgccgaa ccgccgacga acagcaggta gaactcggta ttgatgagcc ccagctcgga
     4861 accgagcaga attgcgccgc cgacgatcca cacccaccag ttcatagcgc cccctgtcct
     4921 gcaccgacag tataggcttc gccgcccccg ggggcccggg ctagaatagg cggatcggct
     4981 ggcgccgctg cagataggcg cgcgccagct gcctcaagcg gagcccatcg aagctcggat
     5041 cgtgaccggc gtggactacc cgggccggca gactctgcag ccgctgcatc gtttccacat
     5101 agacgccgat gtcgctgccc ggaagttcat ccagcagcgg gccgtcgtag atcgcatccc
     5161 cggagaacag cgtggcactc tcggcctccc acagaccgat gctgccgggc gagtggccgg
     5221 gcaggtgcag gacctcaaag cggcggtttc ccaggtcgat cacatcgccc tcttcgaccg
     5281 tgcgcgtcgc gggtgccgcc ttgcgggtaa acacgctcat attgaaaccg gcgtgtggca
     5341 gcgcggtgat gaaggtcggc ccgacctcgt agcccgcccg ccggatcgag ccgatgatct
     5401 cggaagggtg ctcggcgctg tagagactgt agttctcccc ctccaccgcc acgtccgccg
     5461 cctcggcctt gtgaatcacc cgatcggcga actcatggaa agagcccaca tgatcaacgt
     5521 gcgagtgagt cgccaccgcg gtcagcgact tgtcgaacag gtgtcgcgcg gcctcatgca
     5581 ggctcgcgac accgaagccg gtatcgatca gcagatcgcg atcgcgcccg cggacgtgcc
     5641 agatattaca ttgcatcagc cgcagggcgt gcggctccca gatgcgtgtg atgtcatccg
     5701 agagacgctc gaactcgaac cagcgttgcg cgatcggaag tcccacgtgt caggccactt
     5761 tcgacggcgc cagtgccgca aacgccgcca gcacctgggc actggtgcag cgggctccac
     5821 tctgcaggta ctccatcgcc accagcgccg catcgtgagc cg
//
LOCUS       DATMRM010000498         2566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_278875, whole genome shotgun sequence.
ACCESSION   DATMRM010000498 DATMRM010000000
VERSION     DATMRM010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278875"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VNZ06_15190"
     CDS             complement(<1..217)
                     /locus_tag="VNZ06_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HWW22144.1"
                     /translation="MIEYVVPFSELGMHDVARVGGKNASLGEMIGQLTRLGVRVPDGF
                     ATTADAFRDFLAHDGLARRIQSELARLE"
     gene            285..1124
                     /locus_tag="VNZ06_15195"
     CDS             285..1124
                     /locus_tag="VNZ06_15195"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015269492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HWW22145.1"
                     /translation="MVRSAAAVRTVFFVSDHSGVTAETMGHSLLSQFDGISFRAVTLP
                     FISTLERAEEALQKINDTAAIEGTAPIIFSTLVRDAERQVVKRAHGLFLDFFDAFLAP
                     LESELGQLSSHALGRAHGMIDMAGYMTRIDATNFALANDDGGINREYDRADVILIGVS
                     RSGKTPTCLYLALQYGIYAANYPLTEEDLESPQLPPALRQYQQKLFGLTIEAGRLQQI
                     REVRRPDSRYASAQQVQYELRAADQLYERFGLPHIDTTHCSVEEISSRILQLTAIPRR
                     LRP"
     gene            1137..1406
                     /locus_tag="VNZ06_15200"
     CDS             1137..1406
                     /locus_tag="VNZ06_15200"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HWW22146.1"
                     /translation="MSKGCQHLEFVTTSHRNTHGCEECLKMGDSWVHLRLCRSCGHVG
                     CCDDSKNRHATKHFHATQHPIITSIEPGENWSWCYIDRLGLELRE"
     gene            1403..>2566
                     /locus_tag="VNZ06_15205"
     CDS             1403..>2566
                     /locus_tag="VNZ06_15205"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWW22147.1"
                     /translation="MNELMNKRRDQMFPKLSEQQMTRLDTHGVRESVRAGQLLVQQGE
                     RPERFFVVVRGTLEVILARPGDHEVFNTLTAGDFTGEMSMLRGSVGFASIRVGEPGEV
                     IAIDASHLRSIVLTDAELSELFMRAFILRRMGLVAEGAGADVRLLGSRHSGDTLRLQE
                     FLTRNAFPYQDLHIEQHPDVQILLERFHLRSNELPVVVCHGDTVLRNPSNHELAACIG
                     INPRVDDSRVHDLLIVGAGPAGLAAAVYAASEGLDVRMIELMAPGGQAGTSSRIENYL
                     GFPTGISGRALAGRALSQAQKFGANICVACEAVKLHPETRPYVVDLAEGGAIKANVVL
                     VATGAQYRTLDIETPARFLGAGVYYAATNMEARLCDQEEIIIVGGGNSAGQAAV"
BASE COUNT          498 a          805 c          790 g          473 t
ORIGIN      
        1 cctcgagccg cgccagctcc gactgaatac ggcgcgccag cccgtcgtgc gccagaaagt
       61 cgcgaaacgc atccgcggtg gtcgcgaatc cgtccggtac ccgcaccccc aggcgcgtga
      121 gttgaccgat catctcgccc aaagaggcat tcttgccgcc cacgcgggcg acatcgtgca
      181 tgcccagctc tgaaaacggc actacgtatt caatcaatct tgagactcct cagggagagt
      241 gaaacactag cgatgatcat ccgcaatgta tcaggaggca atacgtggtc aggagcgctg
      301 cagccgtccg aacggttttt tttgtgtcgg accattcggg cgtgacggcc gaaaccatgg
      361 gccacagcct tctgagccag ttcgatggca tttccttcag ggcggtcacc ctgcccttca
      421 tcagcaccct cgaacgcgcc gaagaggcgt tgcagaagat caatgatacc gcggcaatcg
      481 agggtaccgc gcccatcatt ttcagcacgc tggtgcgcga cgccgaacgc caggtggtca
      541 aacgcgccca cggcctgttc ctggacttct tcgacgcctt tctggcaccc ctcgagtcgg
      601 aactcgggca gctcagttcg cacgcactcg ggcgcgccca cggcatgatt gacatggcgg
      661 gctacatgac gcgcatcgac gcgacgaact ttgcgctcgc gaacgatgat ggcggcatca
      721 accgggaata cgaccgcgcc gatgtgatcc tgatcggtgt ctcgcgttcc ggcaaaacgc
      781 cgacctgtct gtatctggcc ctgcagtatg gcatctacgc ggccaactat ccgctgaccg
      841 aggaggatct cgagagcccg cagctgccgc cggccctgcg ccagtaccaa cagaagctat
      901 ttggattgac catcgaggcc gggcggctgc agcagatccg cgaagtgcgg cgtccggata
      961 gtcgctacgc ctcggctcag caggtacagt atgagctgcg tgccgccgac caactctacg
     1021 aacgctttgg tctgccacac atcgatacca cgcactgttc cgtcgaggag atctccagcc
     1081 gcatcctgca gctcactgcg atcccgcgtc gtctgcgtcc ttgagagaac gcttgtatgt
     1141 caaaaggctg tcagcacctg gaattcgtca cgacctctca tcgcaatacg cacggctgcg
     1201 aggagtgtct gaagatgggc gatagctggg tgcatctgcg cctgtgccgc agctgtggcc
     1261 acgtcggctg ctgcgatgac tcgaagaacc ggcacgccac caaacacttt cacgcgaccc
     1321 agcatccgat catcacgtcg atcgagccgg gtgagaactg gagctggtgt tatatcgatc
     1381 gcttgggtct ggagctgcgc gaatgaacga gctcatgaac aagcgccggg atcagatgtt
     1441 cccgaagctc agcgaacagc aaatgacgcg actggacacg catggcgtgc gtgagagtgt
     1501 tcgggcgggc caactattgg tgcaacaggg cgagcggcca gagcgttttt tcgtcgtcgt
     1561 ccgcggtacc ctggaagtca ttcttgcccg gccgggggat cacgaagtct tcaacacgct
     1621 caccgcgggt gatttcaccg gcgagatgag catgctgcga ggcagtgtcg gcttcgccag
     1681 cattcgcgtc ggcgagcctg gcgaggtcat tgcgatcgat gcctcgcatc tgcgcagcat
     1741 cgtgctgacc gacgccgagc tcagcgagct gttcatgcgc gctttcattc tgcgccgcat
     1801 ggggctggtg gccgaaggcg ctggcgcgga tgtacgtttg ctcggatcgc gccattcggg
     1861 cgacacgctc agacttcagg agtttctgac acgcaacgct tttccctatc aggatctgca
     1921 catcgaacag catccggatg ttcagatact gctggagcgg tttcacctgc ggtccaacga
     1981 gctgccggta gtcgtatgcc acggcgacac ggtgctcagg aacccgagca atcacgagct
     2041 ggcggcctgc atcggcatca accccagggt ggatgacagc cgcgtccacg acctgctgat
     2101 cgtcggagcc ggccccgcgg gactggccgc ggcggtctac gcggcctccg aggggctgga
     2161 cgtgcggatg atcgaactga tggcaccggg cggacaggcc ggtaccagct cgcgtatcga
     2221 aaactatctg ggattcccta ccggcatttc cggccgggca ctcgccggcc gtgcgctgtc
     2281 gcaagcacag aaattcggcg cgaacatctg cgttgcctgc gaagcggtga aactgcatcc
     2341 ggaaacccgt ccgtacgtcg tggacctggc ggagggcggc gccatcaagg caaacgtcgt
     2401 gctggttgcg accggcgcgc aataccgcac gctcgacatc gagactcccg cgcgatttct
     2461 gggcgcaggc gtctactacg ctgcgaccaa catggaggcg cggctgtgcg atcaggagga
     2521 aatcatcatc gtcggtggcg gcaactcggc cggacaggcc gcggtg
//
LOCUS       DATMRM010000499         6590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2780320, whole genome shotgun sequence.
ACCESSION   DATMRM010000499 DATMRM010000000
VERSION     DATMRM010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2780320"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VNZ06_15210"
     CDS             complement(<1..291)
                     /locus_tag="VNZ06_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWW22148.1"
                     /translation="MGPDGSDLGLVRRVQRGERGAYDLLVLKYQHKVVKLVMRYLREP
                     ADAEDVAQEAFIKAYRALPQFRGDSAFYTWLYRIAINTAKNALAARDRHPVSY"
     gene            complement(473..745)
                     /locus_tag="VNZ06_15215"
     CDS             complement(473..745)
                     /locus_tag="VNZ06_15215"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HWW22149.1"
                     /translation="MPTAGAPRGPAQLLWRCRRGMKELDLMLAGWVHARYEQASEAER
                     SHFLSLLDLPDPELVRYLLGEERPDCPELAKAAAAVRSSAGVMSRP"
     gene            complement(748..1539)
                     /locus_tag="VNZ06_15220"
     CDS             complement(748..1539)
                     /locus_tag="VNZ06_15220"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005096284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HWW22150.1"
                     /translation="MGEKTLPPNSRIDTRAGKIFRAAPGAKRVRTFRIYRFDPVSGER
                     PRVDSYEVDMASCGPMVLDALLKIKNEIDPTLSLRRSCREGICGSCAMNIDSVNTLAC
                     TKACDDVKRTTISIYPLPHMRVVKDLVTDLTDFYAQYAAVKPWLQTRTPPPPDRERLQ
                     SKEDQLQIDRPSACILCACCSTACPSYWWNSERYLGPAALLAAYRWLVDSRDEARGER
                     LDELEDPFRLYRCHTIMNCTEACPKDLNPAKAIGAIKRMLAERRH"
     gene            complement(1544..3328)
                     /gene="sdhA"
                     /locus_tag="VNZ06_15225"
     CDS             complement(1544..3328)
                     /gene="sdhA"
                     /locus_tag="VNZ06_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HWW22151.1"
                     /translation="MSAYELVDHTYDVVVIGAGGAGLRATLGLAEAGLSTANLTKVFP
                     TRSHTVAAQGGISAALGNMGEDDWRWHMYDTVKGSDWLGDQDAIEFMCKEAPAAVIEL
                     EHYGVPFSRTDDGRIYQRPFGGMTTGFGKGIAQRTCAAADRTGHALLHTLYQQSIRHE
                     AEFFIEYFAIDLIMQNDECRGVVAIDLADGRLHRFRAQTTILATGGYGRTYASCTSAH
                     TCTGDGAGMVLRAGLSLQDMEFVQFHPTGMYGSGCLVTEGARGEGGYLTNANGERFME
                     RYAPNAKDLASRDVVSRAEANEIREGRGVGEHKDHIHLHLEHLGAAVLHERLPGITET
                     ARIFTGVDVTKEPIPILPTVHYNMGGVPCTLRGEAVTLRHGDPNCVVPGLMAVGEAAC
                     VSVHGANRLGSNSLLDLVVFGREASRHCAESLKAGTRQRPLPKDGAELALSRLDRLRH
                     AKGSRPTAAIRTEMQKIMQTDAAVFRTGDTLTLGMQKLAATFESFADVGVSDRGLIWN
                     TDLIETLELENLLVQALATVHSAANRKESRGAHAREDFPDRDDANWMKHTLSWVDVNG
                     RVKLDYRPVHMKTLTNEVEVIPPKARTY"
     gene            complement(3325..3711)
                     /gene="sdhD"
                     /locus_tag="VNZ06_15230"
     CDS             complement(3325..3711)
                     /gene="sdhD"
                     /locus_tag="VNZ06_15230"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HWW22152.1"
                     /translation="MSLRAPLARALNHGAAHDGTHHWMVQRVTALAIAPLAIWLLFAL
                     LSLPDSSYATAARWIQHSWNAVPLSLLVLLASWHGWLGMQMVIEDYIDTFLSKTALLL
                     LSAFVFALLAASGVYAVLRVALRGVP"
     gene            complement(3708..4109)
                     /gene="sdhC"
                     /locus_tag="VNZ06_15235"
     CDS             complement(3708..4109)
                     /gene="sdhC"
                     /locus_tag="VNZ06_15235"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HWW22153.1"
                     /translation="MAERPLSPFTKMFGESGYRWAYTMTLSILHRVTGVALVFALLGL
                     VVWLIGASFGPAGYQALLPLFIGVPAKLVISLAVIALIYHFCNGLRHLAWDMGWGFER
                     AAARRSAVLVVFLTAIASSVCILLLFHGSAP"
     gene            4360..5223
                     /locus_tag="VNZ06_15240"
     CDS             4360..5223
                     /locus_tag="VNZ06_15240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22154.1"
                     /translation="MTTHSPGCLLDDLGVIRFRGADTRQFLQGQLSNDIERLSPDALL
                     RASVNTPQGRTLALMGLLADDTDVLALMPRDLIASISTLLKRYVLRSKVTLADDSAAF
                     RIVGLRSTDAIAAGVQPGQLCRYGMQADQRRVLLQPLTTHDEVSFDMARDSWRALDIA
                     AGIPYVAGSSSGEFVAQMLNLDCIDAISFNKGCYTGQEVIARAHFRGRVKRRMQRFVS
                     EAPQTLVAGASGQLQDGRAFRVIDAVTRSDGRCEFLAVAPLPGAAPEATPQSTERLAA
                     HSLPLPYPFPD"
     gene            complement(5229..>6590)
                     /locus_tag="VNZ06_15245"
     CDS             complement(5229..>6590)
                     /locus_tag="VNZ06_15245"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWW22155.1"
                     /translation="AGGVLVFHDVSESRELNRRLSYHASHDLLTGLVNRREFENRLER
                     ALKSAKARETSYALCYLDIDQFKIVNDTCGHSAGDALLGQMGALLKSKVRWRDTLSRL
                     GGDEFGILLEACTLDEAMRMAESLREAVRSFRFSWEERVFRLGASIGVVPITADNEDV
                     ASVLSAADSACAAAKEQGRNRVHSFAENDIELMRRRREMQWAARINAALEEDRFELFR
                     MPIQPLQVVATGAHYELLLRLRDENNRIVSPDNFIAAAERYGIMPSIDRWVIENALRW
                     LVSEADERERLAMCSINLSGQSLGDDKFLPYVIDQFHRSGLDASKICFEITETAAVAS
                     FSQANRFIQALKELGCKFALDDFGTGLSSFGYLKHFPVDYLKIDGSFVREILHDPIDR
                     EMVRSINEIGHLTGKQTIAEFAENAEIIQMLKNIGVDYAQGYGVAQPQRVVRSAASQS
                     GTT"
BASE COUNT         1245 a         2166 c         1975 g         1204 t
ORIGIN      
        1 gtagctgacc ggatgacgat cacgggcagc caacgcattc ttcgcggtat tgatggcgat
       61 gcgatacagc caggtataaa aggcgctgtc gccccggaac tgcggcaagg ctcggtaggc
      121 cttgatgaat gcctcctgag ccacatcttc cgcatccgcc ggttcgcgca ggtagcgcat
      181 cacgagcttg acgaccttgt gctggtactt cagcaccagc agatcgtagg cgccacgctc
      241 gccacgctgc acccttcgca ccagtcctaa gtcgctccca tcggggccca tgggcaatcc
      301 taccactgca ttgccaggcc gccggtcaac gacctgaata gactccaaac gcttttcaaa
      361 gttcgccttc tcgagtttat cagttacgac cgacatgaga ggtgcgtccc aacccggcga
      421 atcaggaggt atttgaacct tagagcccgg cgcgcgcgct taagttccgc cgtcacggtc
      481 tcgacataac accggcgctg gagcgcacgg cagccgccgc tttggccagt tcggggcaat
      541 ccgggcgctc ttcccccaac aggtagcgca ccagttccgg gtccggcagg tccagcagcg
      601 acaggaagtg cgagcgctcc gcctcgctcg cctgctcgta ccgggcgtgc acccacccgg
      661 cgagcatcag gtccagctcc ttcatacccc ggcggcaacg ccatagcagc tgggcgggac
      721 cccggggtgc gccggccgtg ggcatgctca gtgccgtcgt tcggccagca tgcgcttgat
      781 cgctccgatc gccttggcgg gattcagatc cttcggacag gcttcggtgc agttcatgat
      841 cgtgtggcag cgatacagcc ggaagggatc ctccagctcg tccagccgct cacctctggc
      901 ctcatcccgg ctatccacca gccagcgata agcggccagc agcgcggccg gccccagata
      961 gcgctccgag ttccaccagt agctcgggca cgcggtcgaa caacaggcgc acaggatgca
     1021 cgctgacggg cgatcgattt gcagctggtc ctccttggac tgcagccgct cgcgatccgg
     1081 tggcggtggc gtacgggtct gcagccaggg tttcacggcc gcatactgcg cgtagaaatc
     1141 ggtgagatcg gtcaccaggt ccttgaccac ccgcatgtgc ggcagcgggt agatgctgat
     1201 ggtcgtacgc ttcacgtcat cgcacgcctt ggtgcaagcg agcgtattga cgctgtcgat
     1261 gttcatcgcg cacgagccac aaatgccctc gcggcaggag cgacgcagcg acagcgtcgg
     1321 atcgatttcg tttttgatct tcagcagtgc atccagcacc atgggcccgc agctcgccat
     1381 gtccacttca tagctgtcca cgcgcggacg ttcgccgctg acggggtcaa accgatagat
     1441 cctgaaagtg cgcacgcgct tggcacccgg tgccgcacga aagatctttc cggcccgggt
     1501 atcgatgcgc gaattcggcg gtagcgtctt ttcacccatg atttcagtac gtccgcgctt
     1561 tgggcgggat cacttccacc tcgttggtca gagttttcat atgcaccggg cgatagtcga
     1621 gcttcacgcg gccgttgacg tcgacccagg acaaggtgtg tttcatccag ttcgcgtcat
     1681 cgcggtccgg gaaatcctca cgcgcgtgtg caccgcgact ttccttgcga ttggccgctg
     1741 aatgcacggt cgccagtgcc tgcaccagca gattctccag ttccagtgtc tcgatcagat
     1801 cggtattcca gatcaggccc cgatccgaga caccgacgtc ggcaaacgat tcgaacgtcg
     1861 cggccagttt ctgcatgccc agggtcagcg tgtcaccggt acgaaatact gcggcatcgg
     1921 tctgcatgat cttctgcatc tcggtacgga tcgcggcggt cggtcgtgac cctttagcgt
     1981 gacgcagccg gtccaggcgc gacagcgcca gctcagcgcc atctttcggc agcgggcgct
     2041 gccgggtacc ggccttcaga ctctcagcgc agtggcgcga ggcctcgcgg ccgaagacca
     2101 ccagatccag cagcgagttc gaccctaagc gattggcgcc atgcaccgag acgcaggcgg
     2161 cttcgccgac cgccatgaga cccggcacca cgcagttcgg atcaccgtgc ctgagcgtga
     2221 cggcctcgcc gcgaagggtg cacggcacgc cccccatgtt gtagtggacc gtgggcagga
     2281 tcggaatcgg ctctttcgtc acatccacgc cggtgaagat gcgtgcggtc tccgtgatcc
     2341 ccggcaggcg ctcgtgcagc acggccgcac cgagatgttc caggtgcaga tgaatatggt
     2401 ccttgtgctc accgaccccg cgcccttcgc gaatctcgtt ggcctcggcc cgactgacca
     2461 cgtcgcgcga ggccagatcc ttggcattgg gggcatagcg ctccatgaag cgctcgccat
     2521 tggcgttggt cagataccca ccctcgccgc gcgcaccctc ggtcaccagg cagcctgagc
     2581 catacatgcc ggtgggatga aactgcacga actccatatc ttgcagcgac agcccggcgc
     2641 gcagcaccat gccagcgcca tcaccggtgc aggtatgcgc cgaggtacag gacgcgtagg
     2701 tgcgcccata gccgccggtg gccagaatcg tggtttgcgc gcggaagcga tgcagcctgc
     2761 cgtcggccag atcgattgcc accacaccac ggcattcatc gttctgcatg atcaggtcga
     2821 tcgcgaagta ttcgatgaag aattcggcct catgccggat cgactgctga tacagcgtgt
     2881 gtaacagggc atggccggtg cggtccgccg cagcgcaggt gcgctgcgcg atgcctttac
     2941 caaagccggt ggtcatgcca ccgaacgggc gctgatagat gcggccatcg tcggtgcgcg
     3001 agaacgggac gccgtagtgc tcgagttcga tgacggcggc cggggcctcc ttgcacatga
     3061 attcgatggc atcctgatcg cccagccagt ccgagccttt gaccgtgtca tacatatgcc
     3121 agcgccagtc gtcctcgccc atgttgccga gcgcggcgct gatgccgccc tgggcggcga
     3181 cggtatgact gcgggtggga aataccttgg tgagattggc ggtagacagg ccggcctcag
     3241 ccagccccag cgtggcgcgc agacccgcgc cgcccgcgcc aatcaccacc acgtcgtagg
     3301 tatgatcaac caactcgtaa gcgctcacgg cacgccccgc agcgcgacgc gcagcaccgc
     3361 atagacacca ctggccgcga gcaacgcgaa cacgaaagcc gacagcaaca acaacgcagt
     3421 cttcgacaga aacgtgtcga tgtagtcctc aatgaccatc tgcatgccga gccagccatg
     3481 ccagctcgcg agcagcacca gcagcgataa cggcactgca ttccagctgt gctgaatcca
     3541 tcgggctgcc gtggcgtaac tggaatcagg cagcgacagc aatgcgaaca acagccagat
     3601 cgccagcggt gcgatcgcca gcgcggtcac ccgctgcacc atccagtgat gcgtgccatc
     3661 gtgcgcggcg ccgtgattga gggcgcgggc cagcggtgcg cgcaggctca cggtgcgctc
     3721 ccgtgaaaca acaacagaat gcacaccgag gaagctatcg cggtgaggaa caccaccagc
     3781 acggcactgc gtcgtgcggc cgcgcgctca aagccccagc ccatgtccca ggccagatgc
     3841 cgcagaccgt tgcagaagtg atagatgagt gcgatcacgg cgagcgagat cacgagcttc
     3901 gccggcaccc cgatgaacaa cggcagcagt gcctggtaac cggcgggtcc gaagctggcg
     3961 ccgatgagcc acacgaccag cccgagcagg gcaaatacca aagccacgcc ggtgacacga
     4021 tgcaatatcg acagcgtcat cgtgtaggcc caccgatatc cgctttcacc aaacatcttc
     4081 gtgaatggtg acagtggccg ctcagccatc atctctcctc atcaaaaatc gatacagcga
     4141 ccgccctttt cccagtctcc atagcgggtc ggttccggcc cgagcggacc gccgatctca
     4201 acggctttgg acgcgaacct cgcgggcgca gattcggagc tgttggacgt gaggccggcg
     4261 tcgttcgcga ttgattcttc cgtcgggacg ggcacttctg tgggggccgg cataccagtt
     4321 agtgtgccag aattgccgct gtatgacacg acctgtgcga tgaccacaca ctctcccggc
     4381 tgcctgctcg atgatctggg cgtcatacgt tttcgtggcg ccgacacccg tcagtttcta
     4441 cagggccagc tatccaatga catcgaaaga ctcagtccag acgcgctgct gcgcgcgtca
     4501 gtgaacacgc cccagggtcg gacccttgca ctgatgggtc tgctcgccga cgacaccgat
     4561 gtcctggccc ttatgccccg ggatctcatt gccagtatca gtacattgct caaacggtat
     4621 gtactgcgat ctaaagttac gctcgccgat gacagtgccg cgttccgcat cgtgggactg
     4681 cgctcgaccg acgccatcgc agccggcgtg cagccggggc agttgtgtcg ctacggcatg
     4741 caggccgatc agcgccgggt actgctgcaa ccgctcacta cgcacgacga ggtcagcttc
     4801 gatatggcac gcgacagctg gcgagcgctg gatatcgccg ccgggatacc ttatgtagcc
     4861 ggctcgtcgt ccggcgagtt cgtcgcgcag atgctcaatc tggactgcat cgatgcgatc
     4921 tctttcaaca aaggctgcta taccggacag gaagtgatcg cgcgcgcaca ttttcgtggt
     4981 cgggtcaaac ggcgcatgca gcgctttgtc agcgaggcgc cgcagacgct cgtggccggc
     5041 gcatcgggtc aactgcagga tgggcgtgca ttccgggtca tcgatgccgt gacacgctcg
     5101 gatgggcgtt gtgagttcct cgccgtcgcc ccactgccgg gtgccgcgcc ggaagcaacg
     5161 ccgcaatcaa ccgagcgcct cgcagcgcac agtctgcctc taccctaccc gttcccggac
     5221 tgagcgcgtc aggtcgtacc gctctgactg gcagcgctgc gcaccacgcg ctgcggttga
     5281 gccacgccgt agccctgcgc gtagtcgaca ccgatgttct tgagcatctg gatgatttcg
     5341 gcgttctcgg caaactccgc gatcgtctgt ttaccggtca gatgaccgat ctcgttgatc
     5401 gagcgcacca tctcgcgatc gatcggatca tgcaggatct cgcgcacgaa actgccgtca
     5461 atcttcagat agtccaccgg aaagtgcttc agatagccga aggaggacag tccggtaccg
     5521 aagtcatcga gcgcgaactt gcagcccaac tccttcagcg cctgaatgaa gcggttggcc
     5581 tgcgagaaac tggccacggc cgcggtctca gtgatttcga agcagatctt gctggcatcc
     5641 aggccgctac gatggaactg atcgatcacg tacggcagga acttgtcgtc acccagactc
     5701 tggccggaca gattgatcga gcacatcgcc agacgttcac gctcgtcggc ctcggacacc
     5761 agccatcgca gcgcgttctc gatcacccag cggtcgatgc tcggcatgat gccgtagcgt
     5821 tcggcagccg cgatgaagtt gtcgggcgag acgatgcgat tgttctcatc gcgcaggcgc
     5881 agcagcagtt catagtgcgc accggttgcg accacctgta acggctgaat cggcatgcgg
     5941 aacagctcga accgatcctc ttccagcgct gcgttgatgc gcgcggccca ttgcatctcg
     6001 cgccgccgac gcatcagctc gatgtcgttt tccgcaaaac tgtgcacgcg gttgcgaccc
     6061 tgctctttcg ccgcggcgca ggcactgtca gcggccgaaa gaaccgacgc cacgtcttcg
     6121 ttatccgcgg tgatcggcac cacgccgatg ctcgcgccca gccgaaatac gcgctcttcc
     6181 caactgaaac ggaagctgcg gaccgcctcg cgcagcgact cggccatgcg catcgcctca
     6241 tcgagcgtgc aggcctcgag caatatgccg aattcgtcgc cgcccagacg cgacagcgtg
     6301 tcacgccagc gcaccttgga cttcagcagt gcgcccatct gcccgagcag tgcatcgccg
     6361 gcactgtgac cacaggtgtc gttgacgatc ttgaactgat cgatatccag atagcacagc
     6421 gcgtagctgg tctctcgcgc cttggcactc ttgagcgcgc gctccaggcg gttctcgaac
     6481 tcgcgccggt tgaccagtcc ggtcagcagg tcgtggctgg cgtggtagct caagcgacgg
     6541 ttcagctcgc gcgactcact gacgtcgtgg aacaccagca cgccgccggc
//
LOCUS       DATMRM010000500         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3386277, whole genome shotgun sequence.
ACCESSION   DATMRM010000500 DATMRM010000000
VERSION     DATMRM010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3386277"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2215)
                     /locus_tag="VNZ06_15250"
     CDS             complement(<1..>2215)
                     /locus_tag="VNZ06_15250"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWW22156.1"
                     /translation="LSSPLDVFPEFVPSQVDIQTEAPGFAPQQVEQLVTRPIENAVNG
                     ASGLATLRSESIPGLSVVNITFSDGADVHIARQGISERLSQLGSSLPAGVGPPKLSPL
                     VSSTMDLLKIGLVSDKVDAYELRDAADWTIKPRLLAVPGVAHVIVFGGSVRQIQVTPD
                     IEKLASFGVSLTDLSAAVRAALPLRGAGFIDTATQRILIQSPIPAPDADVIAQAVITL
                     RNRTPILVRDVAQVKVAPALRSGDALVMGQPGVMMSLASQYGANTLVTTLAVEKALTE
                     LEPALRARGISVYPALHRPANFIERALGDLQHSLVLAALLIVAILYLFLRDLRAVLIA
                     FTAIPLSLLAAVAVLDRMGLTLNTMTLGGFAVALGVLVDDAIIGIENIMRRLRVNRQS
                     PTPCPRLQVILDASLEVRGPVIYATVVAIAVFLPELFASSVQGHFVGPLALAFIFAVL
                     ASLLVAITTTPALCALMLQPHDAQREAAWLTRLKSWQRAAVSAVGARFGLMSALLLGL
                     VVASLAVLPFVGGTFMPDFREGHFVMQVASSIPGISLEGMLDVGQRISADVLALPYVQ
                     SIEQQVGRAELGEDTWGPHRSEFHVELKRNATVDQTLAQRQLREILDQYPGLQSEVVT
                     FLGDRISESLSGETAQVAIKVFGEDLDALDASGDRISAVLGKIPGIVDLQVKHQVGTP
                     MLSMQLLPPALAMAGLSAQDVLDAIESSYTGEVLGQTFAGTRTVDVSLLLPEGIRH"
BASE COUNT          421 a          729 c          720 g          345 t
ORIGIN      
        1 gtgacggatg ccctccggta gcagcagcga tacatcgacg gtgcgggtac cggcgaaggt
       61 ctgaccgagt acttcgcccg tgtacgagga ctcgatcgca tccagcacat cctgcgcgct
      121 gaggcctgcc atcgcgagcg ccgggggcag cagctgcatc gacagcatcg gcgtgccgac
      181 ctggtgcttg acctgcagat cgacgatacc gggaatcttg cccaggacgg ccgagatccg
      241 gtcgccgctc gcatccagcg catccagatc ctcgccaaac accttgatgg cgacctgcgc
      301 ggtctcaccg ctgaggctct cactgatgcg gtcgccgagg aatgtcacca cttcgctttg
      361 cagtcccggg tattggtcga ggatttcgcg cagctgccgc tgcgcgagcg tctgatcgac
      421 cgtggcgttg cgcttgagct ccacgtgaaa ctcgctacgg tgcggacccc acgtgtcctc
      481 gccgagttcg gcgcggccga cctgctgttc gatgctttgc acgtagggga gggccagtac
      541 gtcagcgctg atgcgttggc cgacgtcgag cattccttcg agcgaaatgc cggggatcga
      601 gctggcgacc tgcatcacga agtggccttc gcgaaaatcg ggcatgaagg ttccgcccac
      661 gaaaggcagt accgccagtg atgcgaccac cagtcccagc agcaacgcgc tcatcagccc
      721 gaatctggcg cccacagcac tgaccgcggc gcgctgccag gatttcagcc gggtgagcca
      781 ggccgcttcg cgctgtgcgt cgtgtggctg cagcatcagc gcgcacagcg ccggcgtcgt
      841 ggtgatcgcc accagcaacg atgccagcac cgcgaatatg aaggccagcg ccagcggacc
      901 gacgaagtgc ccctgaacgc tcgaggcaaa cagttccggc aggaacacgg caatcgccac
      961 gacggtggcg tagatgaccg gaccgcgcac ctcgagcgag gcatccagga tgacctgcag
     1021 ccgcgggcac ggcgtcggcg actgccggtt gacgcgcagc cgccgcatga tgttctcgat
     1081 accaatgatc gcatcatcca ccaggacacc cagcgccacc gcgaagccac cgagcgtcat
     1141 cgtgttgagc gtgagtccca tccgatccag caccgcgacc gccgccagca gcgacagcgg
     1201 aatggcggtg aaagcaatca gcaccgcacg cagatcgcgc aggaacagat acaggatcgc
     1261 aacgatcagc agcgccgcga gcaccagcga gtgctgcagg tcaccgagcg cgcgctcgat
     1321 gaaattggct ggacgatgca gcgccggata gacactgatc ccgcgggcac gcagcgcagg
     1381 ttccagctcc gtgagcgcct tttccaccgc cagcgtggtc acgagcgtgt tggcgccgta
     1441 ctggctggcg agcgacatca tgacgccggg ctgacccatg accagcgcat cgccggatcg
     1501 cagcgccggc gcaaccttca cctgcgcgac atcgcgtacc aggatcggtg tccggttgcg
     1561 cagggtgatc acggcctgag cgatgacgtc cgcatcgggc gccgggatcg gtgactggat
     1621 gaggatgcgc tgcgtagcgg tgtcgatgaa tccggcaccg cgcagcggca gtgccgcgcg
     1681 gacggcggcc gatagatccg tgagcgacac accgaagctt gccagctttt cgatatcggg
     1741 ggtcacctga atctgccgca cgctgccgcc gaacacgatc acatgcgcga ccccgggaac
     1801 tgccagcagc cgcggcttga tcgtccagtc cgcggcgtcg cgcagctcgt aggcatccac
     1861 cttgtcggac accaggccaa tcttcagcag gtccatcgtg ctggatacca gcggtgagag
     1921 tttgggcggc ccgaccccgg ccggaagact gctgccgagc tgcgacagac gctcggagat
     1981 gccctgacgg gcaatgtgaa cgtcggcgcc atccgagaag gtgatgttga cgaccgacag
     2041 acccgggatc gactcggagc gcagcgtcgc gagcccggag gcgccattga ccgcattctc
     2101 gatcggccgg gtgaccagtt gctcgacctg ttgtggcgcg aaccccggtg cctcggtctg
     2161 aatgtccacc tgcgagggaa cgaactccgg gaatacatcg agcggtgagc tcagt
//
LOCUS       DATMRM010000501        10402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3705328, whole genome shotgun sequence.
ACCESSION   DATMRM010000501 DATMRM010000000
VERSION     DATMRM010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10402
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3705328"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..584
                     /locus_tag="VNZ06_15255"
     CDS             81..584
                     /locus_tag="VNZ06_15255"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWW22157.1"
                     /translation="MQRNLARLSALVLALGLSALASAASVGPGTSAPAFQLPAAAGNA
                     VGLGDLKGQVVLINFWASWCGPCRQEMPILEQMYRKYKTAGFTLLGVNVEPKSTDAVG
                     FLKSTPVTFPILFDTQSKVSTLYEVSGMPSTVIVDRKGKVRYIHHGYKPGDESEYLDQ
                     IRTLMRE"
     gene            640..840
                     /locus_tag="VNZ06_15260"
     CDS             640..840
                     /locus_tag="VNZ06_15260"
                     /inference="COORDINATES: protein motif:HMM:NF025452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HWW22158.1"
                     /translation="MLAALAFAGCSSIQPWVKPYEREHLADPIMSFDRNPIASVYLDH
                     VFEVREGAHGATGSVGGGCGCN"
     gene            853..2019
                     /locus_tag="VNZ06_15265"
     CDS             853..2019
                     /locus_tag="VNZ06_15265"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HWW22159.1"
                     /translation="MNPFRTRYARAAGAALVAVVTLVIVTRADVLPEDRSDVMFHRYE
                     GGGITVEGPSVLVRKKIGDSFSVSYQYYVDLITSASIDVLSQASKYKERRTQNNVGLD
                     YLHGNTLYSIGWINSDEPDYKSNTAFAGISQSMFGDLTTVSFGFSRGWDKVGEVDHGI
                     FQKFVGTADHRTWSLGISQVLTRNMLLGLNFETDENQGYLRSPYRGIRFVDPSSARGY
                     SFGEANTPATRTGNAASGQIKYYLPWHAAVDGNYRFYTDTWGIQANTVKLGYTQPLFR
                     SWTFDALARYYWQGHATFYSDLFPFANAQNFESRDRELAQFHSLTLGLGTAYEFHPAW
                     PRFLERGQINLDVQHIRIDYQDFHNNLLTSPIPGDEPLYAINANVMQFFISFWY"
     gene            2064..2900
                     /locus_tag="VNZ06_15270"
     CDS             2064..2900
                     /locus_tag="VNZ06_15270"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HWW22160.1"
                     /translation="MTKAHFLTALLSAGMLLAPATQALAQSAEQSREEARLITATQVL
                     DELRETPDNRVPDWLMQRAYGVAVIPEVIKGAFIFGGRYGNGVMIVRDSHDRFGNPIF
                     VKLIGGSVGWQVGATSTDVVLIFVTPRSVQNFARGKFTLGVDASVAAGPMGRQGEAAA
                     GVNAEVYSYSRSRGLFAGVALDGTALAFDKRANEFFYNNESVTTEMITSGKVTAHSET
                     AQRFVNAVMVSTKDNQPPGPGSTTPSAPPPNSAPPAAGAQPPSGVKTFPIEDPHPGGE
                     PK"
     gene            2982..3569
                     /locus_tag="VNZ06_15275"
     CDS             2982..3569
                     /locus_tag="VNZ06_15275"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWW22161.1"
                     /translation="MAAEIILGLTLIMSAIGFNKPVVIQYNLFRPYWFLRKRQYWTLI
                     SSGFVHADVGHLLFNLLTFYFFAFRLQQAIGPSRFTALYLIALVLSNTGTYLKRRGEP
                     EYACLGASGAILAVMFAEIVYFPTSRIAFLFLPIPIPAPLFAVLYLAYTIYSARQATD
                     RINHDAHLDGALTGLIFVGLTDWPAWLRAWHVILG"
     gene            3579..4463
                     /locus_tag="VNZ06_15280"
     CDS             3579..4463
                     /locus_tag="VNZ06_15280"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HWW22162.1"
                     /translation="MPARPLPPKHRPRRGHSISGRQFVSSGLPRQFWQDLYHSFMTVS
                     WATVFLSFATFFLIFNLIFAGIYAIRPQDIANLNPPNYWGLFFFSVETFATVGYGDMH
                     PQAPFAHVIAAIEIFIGLMSLSLLTGMMFARFSRPTARFLFARNAVIRPLDGKMTLML
                     RAANARQNIVMEAQATLRLLRDETLPEGVQLRRIYDLKLLRQQHPIFLFGWTLMHVID
                     ESSALHGETADSLARSQAYLLLTISGTDETTGQTLMARQNYANDAVRWNHGFADILTT
                     EDGVDYFDYNRFHQIDPL"
     gene            complement(4497..5477)
                     /locus_tag="VNZ06_15285"
     CDS             complement(4497..5477)
                     /locus_tag="VNZ06_15285"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWW22163.1"
                     /translation="MNAVVHDSAIDDAPLLDAYSRTVVGVAEGSTSAVVAIHTRRTPE
                     RAQRGPQGGSASGFVFTPDGLILTNSHVVHGASALRVASVRGEEFDADLVGEDPHTDT
                     ALIRVAASAMPAVTLGSSHGLRVGQLAIAIGNPLGFDCTVTAGVVSALGRSLRASSGR
                     LIEDVIQTDAALNPGNSGGPLMDSSGRVIGMNTAIIAGAQGICFSIAIDTVRRVALEL
                     LRHGRVRRASIGIGGQTTMIPQRLRRHFELPKASGVRVLSVIEDSAAAAAELQSGDLL
                     VRFGAQWIEGVDDLHRLLIDSSIGAAITLEVLRRGRRLTIEVTPREIAAD"
     gene            complement(5474..5704)
                     /locus_tag="VNZ06_15290"
     CDS             complement(5474..5704)
                     /locus_tag="VNZ06_15290"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWW22164.1"
                     /translation="MTVNLSAQRAPARLTSREREVLALVATGISNKGIARQLQVSPNT
                     VKFHIAAILDKLDAATRAEAVAAALRSGELSL"
     gene            complement(5745..6656)
                     /locus_tag="VNZ06_15295"
     CDS             complement(5745..6656)
                     /locus_tag="VNZ06_15295"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HWW22165.1"
                     /translation="MSQISVLQEWSQARSSLSASLAPRAVSIVCGKHRAISGFHWRPD
                     LIVTAAEALGGDEEVGVLSAAGEQRARVLACDLTTDVAVLQVPGSAEPASASVSSTPL
                     QAGAGVVFVGRSRGGPLVSFGSVRLAGPAWSSRRGGTIDQRLEFEGQLDVRFEGALVA
                     DFAGAAQAMLVAGPRGSLLGIPSATIERIASVVEQRGYLPRPYLGLRLQHLWLDEATR
                     TRWGRARRTVAAVAGVESGSPAERAGLAPGDLIEALDGAPVADIEALAARLAGASPGR
                     TLALLRRRGGQTESIAIEVGEWRPAKA"
     gene            complement(6911..8701)
                     /locus_tag="VNZ06_15300"
     CDS             complement(6911..8701)
                     /locus_tag="VNZ06_15300"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HWW22166.1"
                     /translation="MLLPRLIVAAIAAVAAGRCGAAEPASHLWPPPEEAIVWSGPGAN
                     RAGNVTLQLLGFNDFHGNLQIPVTPFARAVGGAAALAAYLKAAAKSAPQRTLILHAGD
                     LVGASPPATRLLRNEPGIEFLNLLADRHCHFGWATRPIDTHDWRRHPNTCNVVGTLGN
                     HEFDAGIGEIHRLLDGGNSPDGPFLEHRYRGSRVPYVCSNVRLRASGQLLLPAYTVVT
                     LGGIPVGVIGAVLRETPSIVPAWAVRDVEFLDEAQSINQAAAELEAQGVHTLIVIIHQ
                     GLTPLVTDHGIEYQGPLRQLVAQLDPDIDVVVSGHTHNLTNTLLPSRSGSPVLVTQAY
                     SYGEAFEQIELQVDPATRDVVAKSARIVPTWADVAPGLPGDSQALRLTERAQALVAAK
                     VGRAVGTLRQPIGRMTNEAGESALGDLVADAQRSATHADIALMNPGGLRADLRGGALN
                     YGDLLTLHPFGNHLVTLDMTGEQLLAVLEQQWPREGGVAGIRILKTSGLRYSWDATKT
                     LGSHVRAACDASGAPIDPIRHYRVTVNDFLAAGGDNFTLLSAAGQGEVGPLDTDALGA
                     YLHEHAPTSAPDGRRLSRADLGGPQVCQPP"
     gene            9129..>10402
                     /locus_tag="VNZ06_15305"
     CDS             9129..>10402
                     /locus_tag="VNZ06_15305"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWW22167.1"
                     /translation="MSTIQRKALPGALILAATAFAVAPPSLRAAGADAAANAENESSL
                     EEVIVTGTRQEGLTAAESAAPIQILSSDALQAAASSPNLMAALSQLVPSLTMQAFGAD
                     MAGQTLQYRLYGLSPNDVLVLVDGKRRHTTSNLAVDVGSPYQGAANVDVNMIPIDAID
                     HIEVYTEGAAAQYGTDAITGVINIILKKNSSGGIVNATRGGYFNGGGVTDDVSGSVGF
                     EPLSGGYFTISGDWYGHGHSNRGGIDERLVNPANLATAPYSNVVNVTGYPYVNMIQGD
                     GEQHWKTTFLNSGAPIGDSANAYFFGSYGEKTANSYENYRPPTETSFTNSAGVTTYPF
                     PFGYNPQESNKETDYQLNAGVKGLLADWHWDLGTGLGGNKSQISTLDTANPQLYIQQG
                     GGVLNLQNIYDGYLKSSQWASTLDVNRDLDVGL"
BASE COUNT         1977 a         3452 c         3129 g         1844 t
ORIGIN      
        1 gacgtcggct tctcgcaact gaagaacaat cttcaggcaa cgatcggagc gacggtgttc
       61 ttctggtgat aggaggcagt atgcaacgca atctcgcacg actttccgct ctggtgctcg
      121 cccttggcct gtcagcgctg gcgagcgcgg ccagtgtcgg accgggtaca tcggcgcccg
      181 cgtttcagct ccccgccgcc gccggcaatg cggtggggct cggcgatctg aaaggccagg
      241 tggtactcat caacttctgg gccagctggt gcgggccgtg ccggcaggaa atgccgatcc
      301 tcgagcagat gtaccgcaag tacaaaacgg ccggcttcac attgctgggt gtcaacgtcg
      361 aaccgaagtc cactgacgcg gtgggcttcc tgaaatccac accggtaacc ttcccgatcc
      421 tgttcgacac gcagagcaag gtcagcactc tgtacgaagt ctctggcatg ccgagcacgg
      481 ttatcgtgga tcgcaagggc aaggtgcgct acatccacca cggctataag ccgggcgacg
      541 agagtgaata cctcgaccag atccgcacgc tgatgcgaga atagtccgtc gccgccgcaa
      601 ggaatgagca tgaatcgagt ctgtagaggg tgcgcgttgg tgcttgctgc cctggcgttc
      661 gccggctgca gctcgattca gccatgggtc aagccttacg agcgcgagca cctggccgat
      721 ccgatcatgt cgttcgaccg caatccgatc gccagcgtgt acctcgacca tgtgttcgag
      781 gtgcgcgagg gtgcgcacgg tgccaccggc agtgtcggtg gcgggtgcgg ctgtaactga
      841 caccgcccgc tcgtgaatcc tttccggaca cgatacgcgc gagctgccgg cgcagccctg
      901 gtcgcggttg tcacgctcgt catcgtgacg cgcgcggacg tactgcctga ggaccgcagc
      961 gatgtgatgt tccatcgcta cgaaggcggc ggcatcacgg tcgaaggccc ctcggtcctg
     1021 gtccgcaaga agatcggaga ctccttctcg gtctcctatc agtactacgt ggacctgatc
     1081 accagcgcct cgattgacgt gctctcccag gccagcaagt acaaggaacg gcgcacgcag
     1141 aacaacgtcg gactggacta cctgcacggc aatacgctct acagcatcgg ctggatcaac
     1201 agcgacgagc cggactacaa gtccaacacc gcttttgccg gcatcagtca aagcatgttc
     1261 ggcgacctga cgacggtctc cttcggcttc agccgcggct gggacaaggt cggcgaagtg
     1321 gaccacggca tcttccagaa gttcgtcggc accgcggacc atcgcacctg gagcctgggt
     1381 atctctcagg tgctgacccg aaacatgctg ctgggactga acttcgagac cgacgaaaac
     1441 caaggctatc tgcgtagccc ctaccgcggc atacgcttcg tcgatccctc ctcggcccga
     1501 ggctacagtt ttggcgaggc caacacccca gccacgcgta ccggcaacgc cgcttccggg
     1561 cagatcaagt actacctgcc ctggcacgcc gcggtggacg gcaactaccg cttctacacc
     1621 gacacctggg gcattcaggc gaacaccgtc aagctgggct acacccagcc gctgtttcga
     1681 agctggacgt tcgacgcgct ggcgcgctac tactggcaag gtcatgccac cttctacagc
     1741 gacctgttcc cgttcgcgaa tgcgcagaat ttcgagtcgc gcgaccgcga actggcgcag
     1801 ttccacagcc tgacgctcgg cctgggcacc gcctacgaat ttcatcctgc ctggccgcga
     1861 tttctcgagc gcgggcagat caatctcgac gtccagcaca tccgaatcga ctaccaggac
     1921 ttccacaaca atctgctgac cagcccgatt ccaggcgacg agccgctgta cgcgatcaac
     1981 gccaatgtca tgcagttctt tatttccttc tggtactgaa cgatcattcg cctcctaggg
     2041 gtttgatgcc ctcggaggcg aacatgacca aagctcattt tcttacggcg ttgttgtccg
     2101 ctgggatgct gctggcgcca gccacccagg cactcgctca gtcagcggag cagtcgcgcg
     2161 aagaggcgcg gctcatcaca gcgacgcagg tgctcgatga actgcgtgaa acaccggaca
     2221 accgcgtgcc cgactggctc atgcagcgtg cctacggggt tgcggtgatc ccggaagtca
     2281 tcaagggcgc cttcattttc ggcggccgct acggcaatgg cgtgatgatc gttcgcgact
     2341 cgcacgaccg cttcggcaat cccattttcg tcaagctgat cggcggcagc gtcggctggc
     2401 aggtgggcgc cacctccact gacgtcgtgc tgatctttgt taccccgcgc agcgtacaga
     2461 acttcgcgcg cggtaaattc acgctcggtg tcgacgcatc ggtcgctgcg ggcccgatgg
     2521 ggcgtcaggg tgaggccgcg gccggggtga atgccgaggt ctattcctac tcgcgttcgc
     2581 gcggcctgtt tgccggtgtc gcgctcgacg gcacagcgct ggccttcgac aagcgggcga
     2641 atgagttctt ctacaacaat gagtcggtca cgaccgagat gattaccagc ggcaaggtca
     2701 cggcgcactc ggaaacggcg caacgcttcg tcaacgcagt gatggtcagc accaaggaca
     2761 accagccgcc cgggccgggc tccacgacac catcggcgcc accgccgaac agcgcaccgc
     2821 cagcggcggg agcgcagccc ccgagcggcg tgaagacgtt cccgatcgag gatccgcacc
     2881 cgggcggcga accgaagtag gttcgcgcga acctcacgac ttcgccggcg agttcgcgcg
     2941 gcggcgcgcc gcgcgtgcgc tgaagcaacg agaaattcgg gatggccgcg gaaatcattc
     3001 ttggactgac gctgatcatg agcgccatcg gcttcaacaa gcctgtcgtg atccagtaca
     3061 acctgttccg gccctattgg ttcctgcgca agcgccagta ctggaccctc atcagcagcg
     3121 gcttcgtgca tgctgatgtc gggcacctgc tgttcaatct gctgacgttc tacttttttg
     3181 cgttccgact gcagcaggcg atcgggccca gccgattcac ggcgctctac ctgatcgccc
     3241 tggtgctcag caataccggc acatacctca agcgccgggg cgaaccggaa tacgcctgcc
     3301 tgggagcgtc cggggcaatt ctcgcggtca tgtttgccga aatcgtctac ttccccacca
     3361 gcaggatcgc gtttttgttt ctgccgattc ccattccggc accgctgttc gccgtgctct
     3421 acctggcata cacgatctac tcggcgcgcc aggccacgga tcgtatcaac catgacgcac
     3481 acctggatgg cgcgctgacg gggctgatat ttgtcgggct caccgactgg cccgcctggc
     3541 ttcgagcctg gcacgtgatc ctcggctaga cttgccgcgt gcccgcaaga cccctacccc
     3601 ccaagcatcg cccgcgcagg gggcacagca tctcaggacg ccagttcgtc tcgagcggcc
     3661 tgccacgaca gttctggcag gatctgtatc actccttcat gacggtgagc tgggcgaccg
     3721 tcttcctgag cttcgcgacg ttcttcctga tcttcaacct gatatttgcc ggaatctacg
     3781 ccatccggcc ccaggacatc gccaatctga atccgccgaa ctactggggg ttgttcttct
     3841 tcagcgtcga gaccttcgcc accgtgggtt acggcgacat gcatccgcag gcaccgttcg
     3901 cacacgtgat cgcggccatc gagatcttca tcggcctcat gagcctgtcg ctgctgaccg
     3961 gaatgatgtt cgcgcgcttc tcgcgcccga ccgcgcgctt cctgttcgca cgcaatgccg
     4021 tgattcgccc gctggacgga aaaatgacac tgatgctgcg cgctgccaat gcccggcaga
     4081 atatcgtgat ggaagcgcag gccacgctgc gcttgctacg cgacgagacg ctccccgaag
     4141 gcgtgcagct gcgccgtatc tacgatctga agctattgcg tcagcagcac ccgatctttc
     4201 tgttcggctg gaccctgatg catgtgatcg acgagtccag tgcattgcat ggtgaaacgg
     4261 cggattcgct cgcccgatcg caggcctatc tgctgctgac catcagcggc accgacgaga
     4321 ccaccgggca gacgttgatg gcgcggcaaa actacgcgaa cgatgccgtg cgctggaacc
     4381 atgggttcgc cgacattctc acgaccgagg atggcgtgga ctacttcgac tacaaccgct
     4441 tccaccagat cgatccactc tgacgggcga agcgagcgca cgccgcgctg acctgttcag
     4501 tccgctgcga tctcgcgcgg cgtcacctcg atcgtgagcc gtcgcccacg gcgcagtact
     4561 tccagcgtaa tggccgcacc gatgctggaa tcgataagca gtcgatgcag gtcgtccacg
     4621 ccctcaatcc actgtgcgcc gaagcgcacc agcagatcac cgctctgcag ctctgccgcc
     4681 gcggctgcgc tgtcctcgat cacgctcaac acgcgcacgc cggatgcttt cggcagctcg
     4741 aaatgccgcc gcagccgctg cgggatcatg gtcgtctgac cgccgatgcc gatcgacgcc
     4801 cggcgcaccc ggccgtgacg cagcagctcc agcgccacgc gccgcacggt atcgattgcg
     4861 atcgaaaagc agatcccctg ggccccggca atgatcgccg tgttcatgcc gatcacacga
     4921 ccactcgaat ccatcagcgg tccgccggaa ttgcctgggt tcaatgcggc gtcggtctga
     4981 atgacatctt cgatcagccg ccccgaactg gcgcgcagcg agcgtccgag cgcgctgacg
     5041 acgcccgccg tcacggtgca gtcgaacccg agtggattgc cgatcgcgat ggcgagctgg
     5101 ccgacacgca ggccgtgcga actgccgagc gtcacggctg gcatcgcgga ggccgcgacc
     5161 cgaatcagcg cggtatcggt atgagggtcc tccccgacca ggtccgcatc aaactcctcg
     5221 ccgcgcacgc tcgccacgcg cagcgcgctc gccccgtgga ccacatggct gttggtcaaa
     5281 atcaaaccgt caggcgtgaa cacgaagccc gaggcgctgc caccctgagg cccgcgctgt
     5341 gcgcgttcgg gcgtgcgtcg cgtatggatg gccacgaccg ctgaggtcga tccttctgcc
     5401 accccgacga cagtgcgcga ataggcatcg agcagcgggg cgtcgtcaat ggcactgtcg
     5461 tgcaccacgg cattcacagt gacagctctc cgctgcgcag cgccgccgcc acggcttcgg
     5521 cgcgggtggc ggcgtcgagt ttgtccagga tcgcggcaat atgaaatttc acggtattcg
     5581 gcgacacctg cagctgccgt gcgatgccct tgttggaaat gccggtcgcc accaacgcca
     5641 gtacctcgcg ttcacgcgaa gtgaggcggg ccggggcgcg ttgcgcggag aggttcactg
     5701 tcatcgttat ggagacacct gaagccgact acaaggagcg gacgtcaggc tttggctggc
     5761 cgccactctc cgacttcgat cgcgatgctc tcggtctgcc cgccgcgtcg gcgcagcaac
     5821 gccagcgtgc gccccgggct ggcgcccgcc agtctggccg ccagggcttc gatatcagcg
     5881 accggcgccc cgtccagcgc ctcgatcaga tccccgggcg caagcccggc gcgctccgcc
     5941 ggcgagccgc tctcaacgcc agccactgct gcgaccgtcc gtcgggcacg gccccagcgc
     6001 gttcgcgtcg cctcatcgag ccacagatgc tgcaatcgca gcccgagata cggtcggggc
     6061 agatagccgc gctgttcgac cacgctcgcg atgcgctcga tggtcgcgga cggtatgccc
     6121 agcaagctcc cgcgcggccc cgcgaccagc atcgcctgag cggcgccggc gaagtcagcc
     6181 accagtgcgc cctcgaatcg gacatcgagc tggccttcga actccagacg ctgatcgatc
     6241 gtaccgccgc ggcgcgacga ccaggcgggc cccgccagac gcaccgaacc aaaactgacc
     6301 agcgggccgc cacgtgagcg gccgacaaag accacaccgg cccctgcttg caatggcgtc
     6361 gatgacaccg aggcagaagc tggctcggca ctgccgggaa cttgcagcac tgcgacatcg
     6421 gtcgtcagat cgcatgccag cacccgcgcc cgctgctcgc cggccgccga cagtaccccg
     6481 acctcttcat caccgccaag ggcctccgcc gctgtgacga tcagatccgg ccgccagtga
     6541 aagcctgaga tcgcgcggtg ctttccgcag acgatgctga cagcgcgggg tgcgagcgat
     6601 gcggacaggc tggagcgcgc ctgcgaccat tcctgcaaaa ccgagatttg gctcatgggt
     6661 gacctcgtca gagaagtgag gtcaaagatg cccagcgcgc tgcgggggcg gtactacccg
     6721 aacgggcagg actgaaaaaa aaatgaggcg gcatcgccgc ctgggtcttg ccctggctga
     6781 cgatgccgcc ccgcttcccc ccgcatccag tacgcgccgt gcgtcgatta atagcaacat
     6841 cgggcaggcg gctttcgcag cggactctaa cactatcggc gaccaatttg cagcaaaaca
     6901 acacgatccg tcagggcggt tgacacacct gcggtccgcc cagatcggcg cggctaaggc
     6961 gccggccgtc cggagcgctg gtcggcgcat gctcatgcag gtatgcgccc agcgcgtccg
     7021 tgtccaaggg cccgacttcg ccctggccgg cggcggacag aagcgtgaaa ttatcgccgc
     7081 cggcggccag aaaatcattg acggtcaccc ggtagtgacg gatcggatcg atcggcgcac
     7141 cgctggcgtc gcaagcagct cgcacgtgcg agcccagcgt tttggtcgca tcccagctat
     7201 aacgcagccc ggacgtcttg agaattcgga tccccgcgac accgccctcg cgcggccatt
     7261 gctgctcgag cacggccaac agctgctcac cggtcatatc caacgtcacc agatgattgc
     7321 cgaagggatg cagcgtgagc agatcgccgt agttgagtgc tccgcctctc agatccgcac
     7381 gcagtccgcc agggttcatc agcgcgatgt cagcatgggt cgccgagcgc tgagcatcgg
     7441 cgaccaaatc cccgagtgcg gactccccgg cctcgttcgt catccgtccg atcggctgcc
     7501 gcagagtgcc cactgcccgg cctaccttcg ctgcgaccaa tgcctgggcg cgctcagtca
     7561 ggcgcagggc ctgtgaatcg cccggcaatc cgggagcgac atccgcccag gtcggcacga
     7621 tgcgcgctga ctttgcgacc acgtcgcggg tggccgggtc aacctgcagt tcgatctgtt
     7681 caaatgcctc gccgtatgag tacgcctggg tcaccagcac cgggctgccg ctgcggctgg
     7741 gaagcaatgt gttggtcaga ttgtgcgtat gaccggaaac cacaacgtcg atgtccggat
     7801 caagctgcgc aacgagctgg cgcagcggcc cctgatactc gatgccgtga tcggtgacga
     7861 gcggcgtcaa tccctggtga atgatcacga tcagagtgtg aacgccctgt gcttcaagct
     7921 cggccgccgc ctgattgatc gactgcgctt cgtccaggaa ttccacgtcg cgaacggccc
     7981 atgcgggcac gatactcggc gtctcgcgca ataccgcgcc gatcacgccg accggaatcc
     8041 cgcccagcgt caccacagtg taggccggca gcagcagctg accgctcgcg cgcagccgca
     8101 cgttcgaaca cacataaggc acgcggctgc cgcggtaccg gtgctcgagg aagggaccgt
     8161 caggtgagtt gccgccgtcg agcagacgat gaatctcgcc gatgcccgca tcgaactcgt
     8221 ggttgccgag tgtgccgacc acgttgcagg tattcggatg tcggcgccaa tcgtgagtgt
     8281 cgatcggccg cgttgcccag ccgaaatgac agtgtcgatc ggccagcagg ttcaggaact
     8341 caatgccagg ctcgttgcgc agcagcctcg tcgccggtgg gctggctccg accagatcgc
     8401 cggcatgcag gatcagcgtg cgctgcggcg cgctctttgc cgcggccttc aggtaggccg
     8461 cgagcgcggc cgcgccgccg accgcgcgtg cgaaaggcgt caccggaatc tgcaaattgc
     8521 cgtggaaatc gttgaagccg agaagctgca gggtcacgtt tccggcacgg ttggcgcccg
     8581 gcccggacca cacaatcgcc tcttccggcg gcggccatag gtggctggca ggttccgccg
     8641 cgccgcagcg gcctgcggcc acagcagcga tcgcagccac gatcagcctg ggcagcaaca
     8701 tttatcgaac ccaagaatta gagtagattg cgttgtgagt cgcccacaga taagtgttgt
     8761 attggcgaaa ccacgatcgg tgcactggcg tgatcggcca ccgcaccaca attgaacgcc
     8821 atcgaacgac atcacatggg cggacgacgg ggaggcggcg cgagcacatc gccatcacaa
     8881 cacaagacga aaggctcagt aaatacaaca tttttgacct gccgtagccg cgcgtctggc
     8941 gcacacgctc cacagatcat cgcaccgtgc gtccgctggc caggttattg cacaggtgaa
     9001 actgcgttgg aacgcatctt acgactcaag cattagggtg ccgtgacaag gggcgacgca
     9061 ccggaagaac tcaatctgtt gccccgacgg cgtatgtcgt tttcaaacag cttttgggga
     9121 gacagctcat gtctacgatt caaagaaagg cgctaccagg cgctctgata ctggccgcga
     9181 cggccttcgc tgtcgcgcca ccctcactgc gggcggccgg ggcggacgct gccgcgaatg
     9241 ccgagaatga atccagtctt gaggaagtca ttgtcaccgg tacccgacag gagggactca
     9301 cggctgctga gagtgccgcg cccatccaga tcctgagttc cgacgccctg caggccgcgg
     9361 cctccagccc gaacctgatg gccgcgctgt cgcagctggt gccctcgctg acgatgcagg
     9421 cattcggcgc cgacatggcg ggacaaaccc ttcaataccg tctctatggc ctgagtccca
     9481 acgacgtatt ggtactggtg gatggcaagc gccgtcacac cacctccaac ctcgcggtgg
     9541 atgttggctc gccctatcag ggcgccgcaa acgtcgacgt caatatgatc ccaatcgatg
     9601 cgatcgatca catcgaggtc tacaccgaag gtgccgcggc gcaatacggc acggacgcca
     9661 tcaccggcgt catcaatatc attctgaaga agaattcttc cggcggcatc gtcaatgcca
     9721 cccgcggcgg ttacttcaat ggcggcggtg tcaccgacga tgtctccggc agcgtgggct
     9781 tcgaacccct gtctggcggt tacttcacca tcagcggtga ctggtatggt cacggccaca
     9841 gcaaccgcgg tggtatcgat gagcgcctgg tgaatcctgc gaacctcgcg accgctcctt
     9901 actcgaacgt cgtcaatgtc accggttatc cctacgtcaa catgattcag ggtgacggcg
     9961 agcagcactg gaagaccacg ttcctgaaca gtggcgctcc gatcggcgat agcgcgaatg
    10021 cgtacttctt tggttcctac ggcgagaaga ccgccaactc gtacgagaac tatcgcccgc
    10081 cgaccgagac ctcgttcacc aactccgcgg gcgtgacgac ctatccgttc ccgtttggtt
    10141 acaatccgca ggaaagcaac aaggaaaccg actatcagct caacgccggt gtcaagggtc
    10201 tcctggccga ttggcattgg gatctgggca ccgggcttgg cggcaacaag agtcagatct
    10261 caacgctcga taccgccaac ccacagctct atatacaaca gggcggcggg gtcctgaacc
    10321 tgcagaacat ttatgacggc tatctgaaat catcacagtg ggccagcacg ctcgatgtca
    10381 atcgcgacct tgacgtcgga ct
//
LOCUS       DATMRM010000502         3412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_282334, whole genome shotgun sequence.
ACCESSION   DATMRM010000502 DATMRM010000000
VERSION     DATMRM010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282334"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VNZ06_15310"
     CDS             <1..694
                     /locus_tag="VNZ06_15310"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWW22168.1"
                     /translation="LGRNDVIDALDTLRSWRSGGARIAAACTGTFVLAESGLLDGGEA
                     TTTWWLSPVFRQRYPRVHLDANRIVVPSGNALTAGAALSHLDLALWLIRNNSPELAAL
                     VARYLVFDSRPSQSAFAISDHLSHSDPLVERFDRWVREHLNTAIALDDVADALATSKR
                     TLTRRLNAVLGKTPVEYIQDLRVERAVHLLKTSKDSVDRIAEQVGYADGVTLRTLLRR
                     RIGKGVRELRAS"
     gene            complement(1018..2028)
                     /locus_tag="VNZ06_15315"
     CDS             complement(1018..2028)
                     /locus_tag="VNZ06_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWW22169.1"
                     /translation="MEPTIIAIDLATRVFQIHFVEPDTGAIHSKVLKRAQLLPFFANR
                     PVSRVVMEACGSAHHWARQLARLGHDVRLIAAQFVRPFVKSNKNDAADAAAIWEAAQR
                     PGMRFVAVKTEDQQAMLALHRMRQQLVRFRVMQVNQIRGLLYEFGVVLPQGRRASIEA
                     ARAALATLADHLPTMLTESLQDQLSRMLVLDDQIRRIEQRIVEWRRSDEACRRISEIP
                     GVGLLTATAAVSVIGQAKNFRSGREFAAYLGLVPRQSSSGGKIRLGGISKRGDVYLRT
                     LLIHGARAVISSSKHLPERLRLLLTRRPTNVVAVALANKMARTIWALLAYGRTYQATP
                     VQ"
     gene            complement(2220..3215)
                     /locus_tag="VNZ06_15320"
     CDS             complement(2220..3215)
                     /locus_tag="VNZ06_15320"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HWW22170.1"
                     /translation="MADELVQIDGSDHRWFEDRAPACTLLVYVDDATSRIMALHFTAT
                     ESTFSYFEVTRAYLEQHGKPVAFYSDKASVFRSTQMATSGGKGVTHFGRAMFELNIDG
                     LCANSSQAKGRVERANLTLQDRLVKELRLRGIDTQAAANAYAPRFIADYNTRFGKAPR
                     SVFNAHRPLRADEDLDTILTWREPRRVTRTLTLQYDRVIYLLDDTEATRRLVGRYIDV
                     YEYPDGRIEIRADGIALPCRPYDRLSEIDQGAVVDHKRLGHVLQVAQLIQADRDNRRA
                     SGSPSRTNQGQAPRMKERRIGTKKQREITVGDLNTAIGKTTASLQDPALKTSSKR"
BASE COUNT          627 a         1062 c         1023 g          700 t
ORIGIN      
        1 gctcggccgg aacgacgtga tcgatgcgct agataccttg cgctcgtggc gcagtggcgg
       61 cgcccgcatt gcagccgcct gtacgggtac cttcgtgctg gctgaaagcg gcttgctcga
      121 cggtggcgaa gccacgacca cctggtggct ctcgccggtg tttcggcagc gttacccgcg
      181 tgtgcacctg gacgcgaatc gcatcgtcgt tcccagcggc aatgcgctaa cggcaggggc
      241 ggccctgagt cacctcgacc ttgcactctg gttgatccgc aacaacagcc ctgagcttgc
      301 ggcgctcgtg gcccggtacc tcgtattcga ttcgcggccg tcgcagtccg cctttgcgat
      361 ctccgatcac ctgtcccatt cggatccgct cgtcgagcgt ttcgaccgct gggtgcgcga
      421 acacctcaac acggcgatcg cactcgacga cgtcgcggac gcattggcga cgagcaagcg
      481 gacattgacg cgacgtctga acgccgtgct cggtaaaacg cccgtcgagt acatacagga
      541 tctgcgggtc gagcgtgccg ttcatttgct gaaaacgagc aaggacagcg tggatcgcat
      601 cgccgagcag gtcggctacg cagacggcgt cacattgcgc acgctgctgc ggcggcggat
      661 aggtaagggg gtccgtgagt tgcgggcgtc gtgagtctta tcgacttttg gagtataccg
      721 atggccgtgt tcgacgggtc ccacgggaga ccagaaccgg acattactta tatggtcgcc
      781 tcccttttgc aaggcgattg tgcgtggcga ggagggtggt tgcggactta tatccggact
      841 cgatcgcggg aagtgcccgc cggccccgat ggatttcgcc gcaaaggtcc taatctaaac
      901 gagcggactc aaggtccgag aaatgtctca ggcttgccct tgcgcggtcc aacctgtctt
      961 gccatcgttc aatacacctg tgcaaccgta gatgaggtta aatgccttga agcatggcta
     1021 ctgtaccggc gttgcctgat acgttcgccc atacgccagc agcgcccaga tcgttcgcgc
     1081 catcttgttg gccagtgcca cagcgacgac gttcgtagga cgtcgcgtca acagaaggcg
     1141 caatctctcg ggcaggtgct tcgaacttga gataacagct cgagcgccgt gtatgagtag
     1201 cgtccgtaga tacacgtcgc cacgtttgct gatgccgccc agcctgatct tgccgcccga
     1261 gctgctttga cgtggcacca ggccgaggta agccgcaaat tcgcgcccag aacggaagtt
     1321 cttcgcctga ccgatgacag acactgcggc ggtggccgta agcagaccga cgccaggaat
     1381 ctctgagatg cgtcgacagg cttcgtcact gcgtcgccat tcgacaatgc gctgctcgat
     1441 gcgcctgatc tggtcgtcca gcacgagcat tcgtgagagc tggtcctgga ggctttccgt
     1501 gagcatcgtc ggcaaatggt ctgccagcgt tgcaagtgcc gctctggcgg cctcgatcga
     1561 ggcacggcga ccctgcggca ggaccacgcc gaactcgtac agcagaccgc gtatctggtt
     1621 gacctgcatg acgcggaacc gtacgagttg ctgccgcatc cggtgaagcg cgagcatggc
     1681 ctgctggtct tccgtcttga cggccacgaa gcgcatgccc ggacgctgcg cagcttccca
     1741 gattgccgcc gcgtccgcgg catcgttctt gttcgatttg acgaacggcc gcacgaactg
     1801 cgctgcaatg agccgcacat cgtgaccaag ccgcgccagt tgccgtgccc agtgatgagc
     1861 gctgccacag gcctccatca ctacacgtga cactggccgg ttggcaaaga acggtagcaa
     1921 ctgcgcgcgt ttaagaacct tgctgtggat tgcgccggtg tcaggctcaa cgaaatggat
     1981 ctggaagacg cgtgtagcca gatcgatggc aatgatcgtg ggttccattt gattctcctt
     2041 ggcggcagga ctgccgaatc cgcaatgtcg cacgttccgg aagcgagacc gcggcgccgg
     2101 acggaccgtt aaggcgggag gcgaccattc catctataaa gctactacaa cacaatgtcg
     2161 aggccattct gaagtgtcgt agtggcagca aagtagaagt gtcgcggttt gtgcctttgt
     2221 tatctctttg acgaggtttt gagtgcaggg tcctggaggc tggccgtagt tttgccaatc
     2281 gcggtgttca gatccccaac ggtaatctcg cgctgtttct ttgtgccgat cctgcgttcc
     2341 ttcattctcg gcgcctgccc ctgattggtt cgcgatggcg aacctgacgc gcggcggtta
     2401 tcgcgatcgg cctgaatcaa ctgcgcgacc tgcagcacat gacccagccg cttgtgatca
     2461 accacggcgc cctgatcaat ctcggagagc ctgtcgtaag gcctgcaggg aagggcgatg
     2521 ccatccgcgc ggatctcgat gcgcccatcc ggatactcat acacatcgat gtagcgtccg
     2581 accagcctgc gggtcgcttc cgtatcgtcc agcaggtaga tcacccggtc atactgcagc
     2641 gtcagcgtgc gcgtgacccg acgcggttca cgccacgtca ggatggtatc gaggtcctcg
     2701 tccgcccgca gtggccggtg tgcgttaaac acgctcctgg gcgccttgcc aaagcgcgtg
     2761 ttgtagtcgg cgatgaagcg cggcgcatag gcattggccg ctgcctgcgt gtcgatgccc
     2821 cgtagccgca actccttcac cagccggtcc tgcagcgtca ggttcgcgcg ctcgacgcgg
     2881 cccttggcct gactcgaatt cgcacacagc ccatcaatgt tcagctcgaa catcgcccgg
     2941 ccgaagtgcg tcacgccctt gccgccgctc gtcgccatct gcgtcgagcg gaacacgctc
     3001 gccttgtcgc tatagaacgc caccggcttg ccatgctgtt ccagatacgc ccgggtcact
     3061 tcaaagtagc tgaaggtcga ttcggtcgcc gtgaagtgca gcgccatgat ccggctggtt
     3121 gcatcgtcga catacaccag cagcgtgcac gccggcgcgc ggtcctcaaa ccagcggtga
     3181 tcgctgccat cgatctgcac cagttcgtcc gccaactatt tcctctcgaa gcaaaccgac
     3241 ctctatgcct tgctcaacta cgagcacgtg catggcgacg gcgtaaaagc cacgctgttc
     3301 accgcaacgc cttcgagcgg cgccaaccag accatcgtca gcctgggcat ccggcatcgg
     3361 ttctgacgca tcgctgccag ggttttcttg cgaaatgttt ttcgacctgc ga
//
LOCUS       DATMRM010000503         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_2165029, whole genome shotgun sequence.
ACCESSION   DATMRM010000503 DATMRM010000000
VERSION     DATMRM010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2165029"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VNZ06_15325"
     CDS             complement(<1..183)
                     /locus_tag="VNZ06_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22171.1"
                     /translation="MLTGFRRFIAGSENGGSHAQPWLFYFSELRAQFNAVKRTFSLLR
                     LSVRFGDKEMNMKIKHL"
     gene            693..1922
                     /locus_tag="VNZ06_15330"
     CDS             693..1922
                     /locus_tag="VNZ06_15330"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HWW22172.1"
                     /translation="MRTRHVADPRHFSRRRKLDFAILISWFLGCNRACVQTELDRFFA
                     VLHNQAELVRGASAQALSKARQNLPHTVFTDLTQHLLTLVDQHIGMPRWHGLRVVAAD
                     ASDVRLTTRDATRRVIQFAKVFALYLPGVEMMLHATLYGTDTGERQMLFEHLDLLGKN
                     DLLVLDRGYPASWLMAALQMRAIHFCMRIDSIGGAAVTTFLRSGAGEAIVTLPPPNRP
                     DASDYEIERAPVTVRLIRLVTPEGRVRVLATSLLDTQTWPAADFAALYHQRWRIEEAF
                     KRIKHRLALEAVSGLSWLTHQQDLAAKVLSDNLSALLCLEALGERDGQISEHTPTLIC
                     SSTDALLKINRTRAFAHIRQCLPRWLCDGVAPAIDDLTRLLRHIADNVIRFMPDRSRP
                     RPLQPKPHKAFASKAVM"
BASE COUNT          528 a          751 c          724 g          507 t
ORIGIN      
        1 taaatgcttg atcttcatgt tcatctcctt gtcgccaaat cgaacgctta acctaagcag
       61 ggaaaaagtt cgctttaccg cattgaattg agcgcgcaat tcagagaaat aaaacagcca
      121 cggttgtgcg tggctaccgc cgttttcgga gccagcaatg aaacgacgga aacccgtcaa
      181 cacggccgtc tattagggaa tgcagggcaa taacgtcggt gatccgatcg agacaactct
      241 gccgtgacag ggaacaacgc tagggatctt gatttgctcc accaggcaac tccttgccgc
      301 ctggtgtcgg cagacacgtg ctgaagagtg gcacggaaat gtgcgcattg gtgactttgt
      361 gagtcagcag cgcattaaga ttgcgtcgcg tgaatggtta tagaccgcgt ctgagacgaa
      421 taccggacaa aagctaggtg gattttaacg ccggctctat cgaaaactga ggaatgaggt
      481 cgatcaattg ccgtctcgct ttgtgtcatc gagcgtccgc caaagtccgc gtgggggcgg
      541 cgtgccaatc ctatcaactt aagccgcaga ggcttgtaaa cacggggctg agcgggtagg
      601 attcggaatg tcgtaatcct cactatttca cgatatgaac tcgggttatc cctatactga
      661 gcgggttcat cagctacctt cattcggcgg aactgcgtac gcgtcatgtt gccgatccga
      721 ggcatttcag ccggcgccgc aagctggact tcgccatact gatcagctgg tttctcggct
      781 gcaaccgggc ctgcgttcag actgagctcg accgcttctt cgcggtgctg cataaccagg
      841 ccgaactggt gcgaggcgca agcgcccagg cactttcgaa agcccggcag aacctgccgc
      901 acaccgtgtt caccgatctg acgcagcatc tgcttacgct cgtcgaccag cacatcggca
      961 tgccgcgctg gcacgggctg cgcgtggtgg ccgccgacgc gagcgatgtg cggctgacta
     1021 cgcgcgatgc cacgcgtcgc gtgatccagt ttgccaaggt cttcgcgctg tacctgcccg
     1081 gcgtcgagat gatgctgcat gcgacgctgt acggtactga taccggcgag cggcagatgc
     1141 tgttcgagca cctcgatctg ctgggcaaga atgacctgct cgtgctggac cgcggttatc
     1201 ccgcctcctg gctgatggcc gcactccaga tgcgcgcgat tcacttctgc atgcgcattg
     1261 acagcatcgg cggcgctgcc gtcacaacgt tcctgcgcag cggtgcaggc gaggcgatcg
     1321 tgacgttgcc accgccaaac cgcccggacg cgagcgacta cgagattgaa cgtgcgccgg
     1381 tcacggtgcg cctcatccgg ctcgtgacac ccgagggccg cgtgcgtgtg ctggccacca
     1441 gtctgctcga tacccaaaca tggcccgctg cggacttcgc cgccctgtac caccagaggt
     1501 ggcgaattga ggaagcgttc aaacggatca agcatcgatt ggcgctcgag gccgtttccg
     1561 gactgagctg gctgacgcat cagcaggatc tcgccgcgaa ggtcctcagc gacaatctca
     1621 gcgcgctgct gtgcctggaa gcactcggtg agcgcgacgg acagatcagc gaacacacgc
     1681 ccaccttgat ctgttcgtcc accgacgcgc tgctcaagat caaccgcacg cgcgcatttg
     1741 cccacatccg gcagtgcctg ccgcgctggc tgtgcgatgg cgttgcgcct gccatcgatg
     1801 atctgactcg cctgcttcgt catatcgctg acaatgtcat ccgcttcatg cccgaccgta
     1861 gccgacctcg accgctgcag cccaagccac acaaggcatt cgcctccaaa gccgtcatgt
     1921 agctcgcctt aagttgcgag gattgggcgg cgtgctactc cacactctgg tgcgatagag
     1981 cgtgggacgg ccgatcgggc tccctgttgt cggcactgat tttccattcc taagcgctgg
     2041 ctcatcgggc gtcgctaagc cggtggttgc ctgtaagccc acgagcacac ttcgcatgtc
     2101 tcctcgcggc agtgcccgcg tcgggttacg ctattgccag tagtagtcag gccgatacct
     2161 gcgggtacgg gcaatcacag tgcttttgaa accgactgcc cgagcagtag tatttcgtca
     2221 aactgggtta ttaattcgaa acacaagaga aacgccaacg tataacaggg tcccggaaat
     2281 ctgtgaatcc atcgcaagat aggtactgca aaacgcgttc taacggggtc gcgagtcatc
     2341 gcgtaggcga tgataatccc gatcgccgcc ccccgagtat atcggtcgga tagtgcaagc
     2401 cgagataaac gcgcggcagg catatgaaga tcgcggtata caaaagcgcg agcacgccga
     2461 cgcggcgcca gacgagaaaa attcctgccg cgaacgtcat ccacagcatc
//
LOCUS       DATMRM010000504        10065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3708109, whole genome shotgun sequence.
ACCESSION   DATMRM010000504 DATMRM010000000
VERSION     DATMRM010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10065
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3708109"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2731
                     /locus_tag="VNZ06_15335"
     CDS             <1..2731
                     /locus_tag="VNZ06_15335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWW22173.1"
                     /translation="QHFGAPTNLMLFGMAASVLLSLLPQIGEQVDYLRFLPKRQRGKA
                     LSWWTALLTTGPGWVLIGGFKILAGSFLAVFALRHGIPSSQATQPVQMYFVAFRETIG
                     SPLTALLLTGLFVIVCQLKINVTNAYAGSIAWSNFFSRLTHSHPGRVVWLVFNVILAL
                     MLMEIGIFHAIDDVLRIYANFAAGWIGALTADLVINKPLGLSPPYIEFRRAHLYDVNP
                     VGIGALILSISASSLAYLGLFGSVAQILSPFVALLSAFAAAPLIAWCTGGRYYLAREP
                     TGLPPHADALRCTICENVFETADMALCPAYSGPICSLCCTLESRCRDTCKPHGRVAQQ
                     LAGLMTRRLPQRAAAALNTRSGHFAGIFLLCNLITGLILSFIYHEYSGADPGARVLVQ
                     TTLWLVFLSLLVLSGIGAWLIVLAHESRRIAEAESTRQTAMLMEEIEAHRQTDAALQR
                     AKEVAEGANLAKTRYIVGMSHEIRTPLNSIFGYAQLLERGNISHSDNAVRVIRRSAEH
                     LSNLIDGLLDVSKIENGVLKLNRDVVALPEFLDQLVDMFRLQAMAKGLEFHYQAPPWL
                     PAQVHTDQKRLRQIIINLLSNALKYTERGAITLAVRYRNPIAEIEILDTGPGILPADQ
                     HRIFEPFERGSSESARAVPGTGLGLTITKLLTQIMGGEIQVRSSSAGSGFTVKLMLGE
                     VHDRIPLPRATHRFCDYTGPRRKVLLADDDPMHLELVQNLLRSLGFTVFMARDGKTCI
                     QLAQQCQPDLAMVDLSMPDISGWEVTRQLRALPPLASMKVMIVSANAYEYSAGAGVHH
                     DLFVTKPIDLSLLLGQIGALLGLTWIEQGSVDRPDADAEPARSTGDGGRHHFDDLYRL
                     GRIGHVRGIEAKLRELELEDPANEAITAQLRALVSNFDLKRYLNVLENLRANG"
     gene            2718..3626
                     /locus_tag="VNZ06_15340"
     CDS             2718..3626
                     /locus_tag="VNZ06_15340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWW22174.1"
                     /translation="MLTAERTGLRRDTVLVVDDTPETLSLLTDTLDQAGFTVLIATDG
                     ESALDLVDQITPDLILMDARMPGISGFETCKRLKRDKLLAHLPVIFMTGLSETEHVLE
                     GLACGGVDYVTKPLILDELLARIRVHLGNARLAQETHAALDASGRFLIATDRAGRVLW
                     CTPKARTLLTGSKIPAQVIEQSLRARRSDGEATARCLVELDGRRLECSLLSSLGADEL
                     LFRVAELANTDDADQSLLQRAYGLTAREAEVLIWISRGKANRDIGEILDISPRTVNKH
                     LEPIFVKLGVENRAAAAAAAVRTLTL"
     gene            3623..4306
                     /locus_tag="VNZ06_15345"
     CDS             3623..4306
                     /locus_tag="VNZ06_15345"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWW22175.1"
                     /translation="MTFAAIASEPYRWPFDGGFTAQNTALIVIDMQTDFCGIGGYVDK
                     MGYDLSLTRAPIEPIRQLLMQARQRGLQVIHTREGHRQDLSDLPQNKRWRSRQIGAGI
                     GDDGPCGKILVRGEPGWQIIPELAPLAGEIIIDKPGKGSFCATDLELVLHTRGIRNLV
                     LTGITTDVCVHTTMREANDRGFECLLLEDCCGATDYGNHLAALKMVKMQGGVFGAVAS
                     SAALMAVLN"
     gene            4339..5952
                     /locus_tag="VNZ06_15350"
     CDS             4339..5952
                     /locus_tag="VNZ06_15350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HWW22176.1"
                     /translation="MIADIQLVVCLLIVIAVVAIVARRLNIPSPILLVLVGVVLALLP
                     GLPTLELAPDILLLLVLPPVIYSAAVAMSWREFRFNLRPISLLAIGAVIFTTAAVALA
                     CHWLLNLSWGVGFVLGAIVSPPDAVAPLAIARRLNLPRRITIILEGEGLANDASALIL
                     YRFAVAAVGGGLFSFSHAAGTFLAIVAGELAWGIAVGWTMLRLRRWVADPRVEIVLSV
                     LTPFLAYWPPEHLGGSGVLATVTAGLYISWNGLRLISAATRLQGIFFWDFLIYVIEGL
                     VFLLTGLQARPVLAGIRNYTAEELLVSGAVVSVVVLLTRFAWIYPATYLPRWLIPAIS
                     RRDPAPPWQWPFMLGFTGVRGIVSLAAALAIPLTTADGMPFPQRDLLLLLAFAVVLIT
                     LVGQGLTLPYLITALKLSDAGLRENGERRIEELRARILAIEAVLERVRALAADGRIPD
                     AVAERLTIHHTNRLELVESRSIEDERHQERVRLTDEVELRLLSEEREAINQLYVQGRL
                     KDETRRKIERELDLREANLASARSDTATL"
     gene            6179..6865
                     /locus_tag="VNZ06_15355"
     CDS             6179..6865
                     /locus_tag="VNZ06_15355"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HWW22177.1"
                     /translation="MIHIAANPAPIAVDPQRTAVVMIDMQRDFLETGGFGESLGNDLS
                     QLTKVIAPCRALLAGARAARLFIIHTREGHRADLSDAPPAKLQRGSPSHRIGDMGPMG
                     RILIRGEPGHDIIPELSPLPGEPVIDKPGKGAFYATELHAMLQFRAIDALIVCGVTTE
                     VCVHTTVREANDRGFRCVVPSDCCGSYFPEFHDTALQMISAQGGIFGWVSDCATILRA
                     LQFQDVRLTA"
     gene            6889..8481
                     /locus_tag="VNZ06_15360"
     CDS             6889..8481
                     /locus_tag="VNZ06_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22178.1"
                     /translation="MAITKPKLWVAGDWNAYFGFGTNILVNLLTLTGLLKFVLKMPDA
                     LIYGRILPATGLMLALSTLYYAWLAYRLAKSTGRSDVCALPSGISVPHMFVVTFVVML
                     PIAIKTGDPVKGWEAGLTWVFIQSFVLMIGGFIGPVVRRITPRAALLGTLAGVSITFI
                     SLRPAFQVFGTPLIGVLCLAIIMSSWFGGVRYFRGIPAGLVAIAVGMAIAWGSTALGH
                     GIGGMSLQNLTASIANFGFSVPLPAFGHVFSGFTYFGIILVTAIPFGIYDLVEALDNV
                     ESAAAAGDPFPTTRVLTADGVVSLVGCMMGNPFINAVYIGHPGWKAMGGRIGYSAATG
                     ITVIVLAWFGVVSVMMALVPVVAILPILVYIGMLIGAQAFQETPRRHAPAIVLALLPN
                     LAAWTKNQIDGALGAAGTSAATLGLDKLGDQGVLYQGLSVLGSGATLGGLMLAAIAVC
                     AIERRFFKGAGFAAAAAILSFFGLMHSEGIGIGQSPLMSVSYLGVAVLMLVCTKLSAV
                     PMTQHEIEAEHGGDLEPSELGA"
     gene            8495..8932
                     /locus_tag="VNZ06_15365"
     CDS             8495..8932
                     /locus_tag="VNZ06_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22179.1"
                     /translation="MEERVVPFGWTKTTHDLSADRTWSVDELRWAHDYERSLLREWAR
                     FPKAGDVYVALQDIRIKYITHWRQPYNGSGSGTLRKGTRVRVDVFEFNVEPVSVRAEP
                     LDLLLVERQLVDPRERGAKGYNGFSLSIRTEELNRVFALARMP"
     gene            complement(8929..>10065)
                     /locus_tag="VNZ06_15370"
     CDS             complement(8929..>10065)
                     /locus_tag="VNZ06_15370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW22180.1"
                     /translation="IGVCHLLNDMMQSLLPAIYPNLKEDFSLNFRQIGLVTFTYQVTA
                     SLLQPLVGWYSDRRPTPTALPGGVLFSMAGLLILSAASHYGWLLIGAALLGMGSSVFH
                     PESSRVARMAAGTRHGLAQSLFQVGGNAGQALGPLAAALVVVRWGQSSLAFFALLALV
                     SLAILWNVSIWYRHHGLARLHTAAKAINPHALMPKSVAVRGIVVLLVLVFSKYIYMAS
                     LTSYYTFYLIDHFHLSVRNAQLYLFAFTAAIAVGTALGGPFGDRIGRKRVLWISVFGP
                     LPFTLLLPYVNLFWTGPLSVIIGLLMASAFPAIVVFAQELVPGKVGMISGLFFGFAFG
                     VSGLGAAGLGSLADATSIEFVYRVCAVLPLVGLLTAMLPDIEQS"
BASE COUNT         1841 a         3150 c         3129 g         1945 t
ORIGIN      
        1 gcagcacttt ggcgctccga ccaacctgat gttgttcggc atggcggcct cggtactgct
       61 gtcactgctg ccgcagatcg gcgaacaggt ggactacctg cgcttcctgc cgaagcggca
      121 acgcggcaag gcgctcagct ggtggaccgc gctgctgacg acgggccccg ggtgggtgct
      181 gatcggcggc ttcaaaatcc ttgccggctc ctttctcgcg gtcttcgcgc tccgccacgg
      241 tattcccagc agtcaggcca cgcagcccgt acagatgtat ttcgtggcat tccgcgaaac
      301 gatcggctcg ccgctgaccg cgctgctact gaccgggctg ttcgtgatcg tctgtcagct
      361 caagatcaac gtgaccaatg cctatgccgg ctcgatcgcc tggtcgaatt tcttctcacg
      421 cctgacgcac agccacccgg gccgggtcgt gtggctggtt ttcaatgtga tcctcgcact
      481 gatgctgatg gagataggca tctttcatgc gatcgatgac gtattgcgta tctatgccaa
      541 tttcgcggcc ggctggatcg gtgccctgac ggccgatctc gtcatcaaca aacccctggg
      601 gctgagcccg ccttatatcg agtttcgacg cgcacacctg tacgacgtga acccggtggg
      661 gatcggcgcg ctcatactct cgatctcggc gtcgagtctg gcctacctcg gcctgttcgg
      721 atcggtcgcg caaatactct cgccgttcgt cgccctgctc agcgccttcg ctgccgcacc
      781 attgatcgcg tggtgcaccg gcggccgcta ctacctcgcc cgtgagccga cgggtctgcc
      841 gccgcatgcg gacgcattgc gctgcacaat ctgcgagaac gtattcgaga ccgccgacat
      901 ggcattgtgt ccggcgtact cgggaccgat ctgttcgctg tgctgcacgc tcgaatcgcg
      961 ttgtcgcgat acctgcaaac cgcacggacg cgtcgcgcag cagctcgctg ggctgatgac
     1021 gcggcgcttg ccgcaacggg cggcagccgc actcaacacc cgcagcggcc atttcgcggg
     1081 catctttctg ctctgcaatc tgatcaccgg tctgatcctg tcgttcatct atcacgagta
     1141 cagcggcgct gaccccggag cgcgcgtgct cgtgcaaacc acgctgtggc tggtgtttct
     1201 gagcctgctg gtcctgtcag ggatcggcgc ctggctcatc gtgctggcgc acgagagccg
     1261 gcgcatcgcg gaagcggaat caacgcgcca gactgcgatg ctgatggagg agatcgaagc
     1321 acaccgccaa accgacgccg ccctgcagcg cgcaaaggaa gtcgccgagg gggcgaacct
     1381 ggccaaaacg cgttacatcg ttggcatgag ccatgaaatc cgcacgcctc tgaattccat
     1441 cttcggctat gcccagttgc tcgaacgcgg caacatcagc cactctgaca atgccgtgcg
     1501 cgtgatccgc cgcagcgccg agcacctgtc gaatctgatc gacggcctgc tggacgtttc
     1561 gaaaatcgag aacggcgttt tgaagctcaa tcgcgatgtc gtggcattgc ccgagtttct
     1621 cgatcagctg gtggacatgt tccgtctgca ggcgatggca aagggtctgg agttccatta
     1681 tcaggcgccg ccgtggctgc cggcgcaggt ccataccgac cagaagcgtt tgcggcagat
     1741 catcatcaat ctgctgtcga acgcgctcaa gtacaccgag cgcggcgcaa tcactctggc
     1801 ggtgcgttat cgcaatccga tcgcagagat cgagatcctg gacaccggtc ccggcattct
     1861 acccgccgat caacaccgta tcttcgagcc cttcgagcgg ggcagcagcg aatccgcccg
     1921 cgcggtaccg gggacaggtc tcggcctgac gatcaccaag ttgctgaccc agatcatggg
     1981 cggcgagatc caggttcgca gcagcagcgc cggatccggt ttcaccgtca agctgatgct
     2041 aggtgaagta cacgaccgca tcccgctgcc ccgcgctacc caccgtttct gcgactacac
     2101 cggaccgcgt cgcaaggtac tgctggccga tgatgatccg atgcatctgg agctggtgca
     2161 gaacctgctg cgatccctcg gattcaccgt cttcatggcg cgcgatggca agacctgcat
     2221 ccagctggca cagcagtgtc agcctgatct tgcgatggtg gatctgtcga tgccggacat
     2281 ttccggctgg gaagtgaccc gacagctgcg cgcgcttccg ccgctcgcat cgatgaaggt
     2341 gatgatcgtc tcggccaatg cctatgagta ttcggccggt gccggcgttc atcacgatct
     2401 gttcgtcacc aagccgatag atctgtcgtt gttgcttgga cagatcggcg cgctcctggg
     2461 gttgacctgg atcgagcagg ggtcggttga tcggcctgac gccgacgctg agccggcgcg
     2521 cagcacgggc gacggcgggc gacaccactt cgacgatctc taccgtctgg gccggatcgg
     2581 gcatgtgcga ggcatcgagg ccaagttgcg cgaactggag ctcgaggacc cggcgaacga
     2641 ggccatcact gcgcagctgc gcgcactggt gtcaaatttc gatctcaaac gctatctgaa
     2701 cgtgttggag aacctgcgtg ctaacggctg aacgcactgg gctgcgacgc gacactgttt
     2761 tggtcgtcga tgacacaccg gaaacgctca gcctgctgac ggacacgctc gatcaggcgg
     2821 gctttacggt gctgatagcg accgacggcg agagtgcgtt ggatctggtg gatcagatca
     2881 cacccgatct gatactgatg gatgcccgta tgccgggcat cagtggtttt gagacctgca
     2941 agcggctgaa acgcgacaag ctgctggcgc accttcccgt cattttcatg acggggctgt
     3001 ccgagaccga acatgtcctg gaaggtctgg cgtgcggcgg tgtcgactac gtgaccaagc
     3061 cgctgatact cgatgaactg ctcgcgcgca tccgcgttca tctcgggaat gcgcggcttg
     3121 cacaggagac tcacgccgcg ctggatgcca gcgggcgctt cctgatcgcc actgaccgcg
     3181 ccggccgcgt gctgtggtgt acccccaagg cgcgcacgtt gctgactggc tcgaagattc
     3241 cggcacaggt catcgagcaa tcgctgcgcg cacgccgatc ggacggggaa gccactgcgc
     3301 gctgcctggt ggaactcgat ggccggcggc tggaatgctc gctgctgagt tcgttgggcg
     3361 cggatgagtt gttgttccgc gtagccgaac tggccaatac cgacgacgcc gatcaatcgc
     3421 ttctgcagcg cgcgtacgga ctgacggccc gagaggccga ggtcttgatc tggatcagcc
     3481 gcggcaaagc caatcgcgac atcggagaga tccttgacat cagcccacgc accgtcaaca
     3541 aacacctgga gcccatcttc gtgaagctcg gcgttgaaaa ccgtgcagca gcagccgctg
     3601 ccgcggtcag gacactgacg ctatgacctt cgcagcgatc gcgtcggagc cttatcgctg
     3661 gccgttcgac ggcgggttta ctgcacagaa cacggcattg atcgtgatcg acatgcagac
     3721 cgatttctgc ggtatcggcg gttatgtgga caagatgggc tacgatctgt cgctgacacg
     3781 ggcaccgatc gagccgattc gccagctgct gatgcaggcc cgccaacgcg gactgcaggt
     3841 cattcacact cgagaaggcc atcgtcagga cctgtcggat ctgccgcaaa acaagcgctg
     3901 gcgctcgcgt cagattggtg ccggcatcgg cgatgatggt ccgtgcggca agatcctggt
     3961 gcgcggcgaa ccgggctggc aaatcattcc ggaactggca ccgctggcgg gtgagatcat
     4021 catcgacaag ccgggtaagg gatctttctg cgccaccgat ctcgagctgg tgctgcacac
     4081 tcgtggcatc cgcaatctgg tcctgaccgg tatcacgacc gacgtatgcg tccatactac
     4141 aatgcgagag gcaaacgatc ggggattcga atgtctgctg ctcgaggatt gttgtggcgc
     4201 gaccgactac ggcaatcacc tggcggcgct gaaaatggtc aagatgcagg gcggtgtctt
     4261 tggcgcggtg gcgagctcag ctgccctgat ggcggtgctg aactgaccga ttgcgcaggc
     4321 tcgagcttgg actaggcaat gattgccgac atccagctcg tcgtctgcct gctgattgtc
     4381 atcgccgtcg tggccatcgt cgcgcgacgg ctgaatatcc cctcccccat cctgctggta
     4441 ctcgtaggcg tggtgctggc actgctgccg ggcctgccaa cgctcgagct cgcaccggac
     4501 attctcttgc tgttagtgct gcccccggtc atttattcgg cggcggtggc aatgagctgg
     4561 cgcgaattcc gcttcaacct gcgcccgatc tcactgctgg ccatcggtgc ggtcattttc
     4621 accaccgcgg cggtcgccct ggcctgtcac tggctgttga atctctcgtg gggagtaggc
     4681 ttcgtgctgg gcgcgatcgt ctcacccccc gatgccgtcg cgccgctggc gatcgcacgt
     4741 cgactgaacc tgccccgcag aattaccatc atcctcgaag gcgaaggtct ggcgaacgac
     4801 gcgtcggcgc tcatcctcta tcgatttgcg gtagcggcgg tcgggggcgg cttattttcc
     4861 ttcagtcacg cggcaggcac gttcctggcc atcgtggccg gggagctggc gtgggggatc
     4921 gccgtcggct ggacgatgct gcggcttcgg cgttgggtcg ccgatccgcg cgttgagatc
     4981 gttctatcgg tgttgacacc gttcctggcc tattggccac ccgagcatct gggcggatcg
     5041 ggggtgctcg caaccgtaac agccgggctg tatatcagct ggaacggatt gcgtctgatt
     5101 agcgccgcga cgcgactgca gggcattttc ttctgggatt tcctcatcta cgtcatcgag
     5161 ggcctcgtgt tcctgctgac cggcctgcag gcgcgtcccg tcctggcagg catccgcaac
     5221 tacacggctg aggagttgct cgtgtctgga gcggtggtca gcgtggtcgt gttgctcacg
     5281 cgcttcgcct ggatctatcc agcgacctac ctgccacggt ggctgattcc ggccatctcc
     5341 cgccgggatc cggctccgcc gtggcaatgg cccttcatgc tgggcttcac cggcgttcgc
     5401 ggcatcgtgt cacttgccgc cgcactcgcc attcccctca ccacggccga cggaatgccg
     5461 tttccgcagc gcgatctgct gctgctgctg gccttcgccg tggtcctgat caccctggtg
     5521 ggacagggac tgacgttgcc ctacctgatc actgcgctga agctgtcgga tgccggcctg
     5581 cgcgagaatg gtgaaaggag gatcgaggag ctacgggcca gaatcctggc cattgaagcg
     5641 gtcctggagc gcgtgcgggc gctggcagcc gacggcagga ttcccgacgc ggttgccgaa
     5701 cggctcacta tccatcacac caaccgactc gagctggtgg aaagtcgcag catcgaagat
     5761 gagcggcatc aggagcgcgt cagactgacg gacgaagtgg agctgcggct gctgagcgag
     5821 gaacgggaag ccatcaacca gctctatgtg caaggtcgcc tcaaggacga aacacgccgc
     5881 aagatcgaac gcgaactcga tctgcgcgaa gccaatctcg ccagtgcgcg cagcgacacc
     5941 gccacgctgt gagcgcgcgg gcgcgcatca gaccgcaccg caattgggcg ttggcgcctc
     6001 ggacggttgt ctgatccgcg cgcggggctt gccatcctcg ggcaggtgaa actgtaaatc
     6061 ttattgggac ttgcgcgcgc ctggcgcacg cgcacgcgct catggaccac gctcaccgca
     6121 gcgcgtggtc cggttcttgc tttgctccct cgtaaagaaa acaacgacgg acgtagccat
     6181 gatccacatc gctgccaacc cggcacccat cgccgtcgat ccgcaacgga ccgccgtggt
     6241 gatgatcgat atgcagcgcg actttctgga aaccggcgga ttcggcgagt cgctgggcaa
     6301 tgatctgtca caactgacga aggtcatcgc cccgtgccgt gcgctgctgg ccggggcacg
     6361 ggcggcgcgc ctgttcatca tccatacgcg tgaagggcat cgtgccgatc tgagcgacgc
     6421 gccgccggcg aagctgcagc gcggctcgcc ctcccatcgc atcggcgaca tgggacccat
     6481 gggcaggatc ctgatccgcg gcgaaccggg acacgacatc atcccggaac tcagtccgct
     6541 gccgggcgaa ccggtgatcg acaagccggg aaagggcgcg ttctacgcca ccgagctgca
     6601 cgccatgctg cagttccggg ccatcgacgc cctcatcgtc tgcggcgtga ccacggaagt
     6661 ctgtgtgcat accacggtgc gggaagccaa cgaccgaggc tttcgctgcg tggtgccctc
     6721 ggattgttgc ggctcatatt tccccgaatt tcacgacacc gccctgcaaa tgatcagcgc
     6781 ccagggcggc atcttcggat gggtgagcga ctgcgccacc atcttgcggg cactgcaatt
     6841 ccaagacgta cgactcacgg cgtaacgcac cgtcagggag ctacaatcat ggccatcacc
     6901 aaaccaaagc tatgggtcgc cggagactgg aatgcctact tcggcttcgg caccaacatt
     6961 ctggtcaatc tgctgacgct caccgggctg ctgaaattcg ttctgaaaat gcccgacgcg
     7021 ctgatctacg gacgcatcct gccggcgacc ggcctgatgc tggcgctcag cacgctgtac
     7081 tacgcctggc tcgcctatcg gctggcgaag agcacgggcc gcagcgacgt ctgcgcactg
     7141 ccatccggca tcagtgtgcc gcacatgttc gtggtgacgt tcgtggtcat gttgccgatc
     7201 gcgatcaaga ccggcgatcc ggttaaaggc tgggaggccg gcctgacctg ggtgttcatc
     7261 cagagtttcg tcctgatgat cggtggtttt atcgggccag tcgttcgacg gatcacccca
     7321 cgcgccgccc tgctgggtac gttggcgggt gtgtcgatca ccttcatctc actgcgtcct
     7381 gcattccagg tgttcggcac tcccctgatc ggcgtgctgt gcctggcgat catcatgagc
     7441 agctggttcg gcggtgtccg ctatttccgg ggcattcccg caggactggt ggcgatcgcg
     7501 gtgggcatgg cgatcgcctg gggttcgacc gcgctcggtc acggcatcgg tggcatgagt
     7561 ctacagaacc tgacggcctc catcgccaac tttggatttt cggtgccgct gcctgcgttc
     7621 ggccacgtgt tctcgggctt cacctacttc gggatcattc tggtgaccgc catcccgttc
     7681 ggtatctacg acctggtcga agcgctcgac aatgtcgaga gcgccgccgc cgcaggcgac
     7741 ccctttccca ccacgcgtgt gctcactgcc gatggggtgg taagcctggt cggctgcatg
     7801 atgggcaatc ctttcatcaa tgcggtctat atcgggcatc cgggttggaa ggcaatgggt
     7861 ggacgcatcg gctactcggc cgcgaccggc atcacagtga tcgtgctcgc ctggtttggc
     7921 gtcgtgtcgg tgatgatggc gctggttccc gtggtcgcga tcctgccgat cctggtctac
     7981 atcggcatgc tgatcggagc ccaggccttc caggaaaccc cacgacggca cgctccggca
     8041 attgtgctgg cgctgttgcc aaacctggcg gcatggacca agaaccagat cgacggggcg
     8101 ctgggggcag cgggtacctc agctgccacg ctcggactgg acaagcttgg cgatcaggga
     8161 gtgctttacc aggggttgtc cgtactggga agcggcgcga cgctaggagg gctgatgctt
     8221 gctgctatag cggtctgcgc gatcgagcgc cggttcttca agggcgccgg ttttgccgcg
     8281 gccgcggcca ttctgagctt ctttggcctg atgcacagcg agggtatcgg gatcggtcaa
     8341 tctccgttaa tgtcggtgag ctatcttggt gtcgccgtgc tgatgctcgt ctgcaccaag
     8401 ctcagcgccg taccgatgac gcagcatgag attgaggccg aacacggtgg ggacttggaa
     8461 ccctcggagc tcggcgcatg agccaggagc gcgggtggaa gagcgagttg tccctttcgg
     8521 atggaccaag acgacccatg atctgtcagc cgacagaacc tggagtgttg atgagttgcg
     8581 ctgggcacac gactacgagc gctcactgct gcgcgaatgg gctcgtttcc caaaggccgg
     8641 cgacgtgtat gtagcgttgc aggacatccg gatcaagtac atcacccact ggcggcaacc
     8701 ctacaacggc agcggcagcg gaacgcttcg caaaggcacg cgcgtgcgtg tggacgtgtt
     8761 cgagttcaac gttgaacccg tcagcgtacg cgccgaaccg ctcgacctgc tcctggtcga
     8821 gaggcagctg gtggatccac gcgagcgtgg cgccaagggt tacaacggct tcagcctctc
     8881 gatccgaacc gaggaactca accgcgtctt cgcgctggcc aggatgcctt aagactgctc
     8941 gatgtccggc agcatcgccg tcaacaatcc caccagcggt agcacggcgc agacgcggta
     9001 cacgaattcg atactggtcg catccgccag cgaccccaac cccgccgcgc ccaatccgct
     9061 cacaccgaac gcaaaaccga agaacagccc cgagatcatt cccaccttgc ccggtaccag
     9121 ctcctgcgca aatacgacga tcgccgggaa tgcggacgcc atcagcaagc cgatgatgac
     9181 gctcaatggc cccgtccaga acaggttgac atagggcagc agcagagtga acggcagcgg
     9241 cccgaatacc gatatccaaa gcacgcgctt gcgtccgatg cgatcgccaa aaggtccgcc
     9301 cagggcggta ccgaccgcaa tcgctgcggt gaaggcgaac agatacagct gtgcattgcg
     9361 taccgacaga tgaaaatgat cgatgagata gaacgtgtaa tagctggtca ggctcgccat
     9421 atagatgtac ttcgaaaaca ccagtacgag cagcacgacg atgccgcgca ccgcgactga
     9481 cttcggcatc agcgcgtggg gattgatcgc cttggcggcg gtgtgcagtc gtgccagacc
     9541 gtgatgtcgg taccagatgc tcacgttcca caggatcgcc agcgacacca gcgcgagcaa
     9601 tgcgaagaat gcgagactgg attgacccca gcgcacgacc accagcgcgg ccgcgagcgg
     9661 accgagcgcc tgaccggcgt tgccgccgac ctgaaacagc gattgcgcca gtccgtggcg
     9721 cgtacccgca gccatccgcg cgacgcgcga ggactccgga tgaaacaccg aggagcccat
     9781 tcccagcaat gcggcaccga tcaacagcca tccatagtgg ctggccgctg acagaatcag
     9841 cagtcccgcc atcgaaaaca agacgcctcc gggcagcgcc gttggagtcg ggcgacggtc
     9901 ggaataccac cccacgagcg gctgcagcag cgatgccgtc acctggtagg taaaggtcac
     9961 gagtccgatc tgacggaagt tcagactgaa gtcctccttc agattgggat agatcgccgg
    10021 tagcaacgat tgcatcatgt cattcaacag atgacagacg cctat
//
LOCUS       DATMRM010000505         7597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3390310, whole genome shotgun sequence.
ACCESSION   DATMRM010000505 DATMRM010000000
VERSION     DATMRM010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3390310"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1152)
                     /locus_tag="VNZ06_15375"
     CDS             complement(10..1152)
                     /locus_tag="VNZ06_15375"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HWW22181.1"
                     /translation="MSTRATPHLTGLSGNEIYCLRLKGLDPQGVLVGNSVQSIGLLGG
                     LRSAVSGIIGGEVPAVTQVIHDGREAAFQRLVAEARQAGVSGIVGVSSDLRALSRFTE
                     FLFVGSGVHAQPRRELFTSAGDAQELYCHMDAGYEPLQFVFGNIAYSVGAVRSIVGAL
                     RTLGRGEIKEFSDVFNETRHQALGRMAAHAAQAGANCVVGVRTHILRFAGFHEMYMTG
                     TAARHALLPVAGQRAVATSDLTGEELWSLTILGYVPLKLLISTSVYSLGVVGGFMAAL
                     KGFVKGEISELTTLVYEAREQVFDRLDREARALGADRVVGVKTYIVELGTSLIEIFSV
                     GTAVCQIEGPKVATAALPAQAIIRDRDTWVDGEVALDMTDIRAGGK"
     gene            complement(1162..1884)
                     /locus_tag="VNZ06_15380"
     CDS             complement(1162..1884)
                     /locus_tag="VNZ06_15380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWW22182.1"
                     /translation="MSAWLAHALAFYTVLISPWWGRYALRRALRQMRANVPRFRMRWY
                     RLILVEQAIIIAVVVAIWRLGDVPAARLGLVLPKSWSFTLAMLVGVCVGLIIPSFLLR
                     AKAQKLRETPWVKLFVPETRRERAGYAAVSIGAGVSEELWIRGFLIYYLSVLLPPLNL
                     IAIAALTSVVFGLLHFYQGVQGIIATAVLGGLFASFYLTTGSLVLPVVLHAIFDLRLL
                     LMFPLRAERAGAVQSTPDDSQP"
     gene            complement(1887..2291)
                     /locus_tag="VNZ06_15385"
     CDS             complement(1887..2291)
                     /locus_tag="VNZ06_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22183.1"
                     /translation="MTYLALDCQVASNPRVRTASHALHPSVARPFARALWLEEFVDSG
                     RQQVASILINFHYAGERLQAGRYPTRHRQLQRVLKRDSFRKAFETQLPAAKSVGALDL
                     NPFADLGYQRRHVMFRIGDFSRIARVSCRLLR"
     gene            2578..4518
                     /locus_tag="VNZ06_15390"
     CDS             2578..4518
                     /locus_tag="VNZ06_15390"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HWW22184.1"
                     /translation="MTTPGKAPPGSTPASVSDEAKTIWLDLSTPVEVSPQTVDRIMDK
                     GSTLNGRYRLLELIGEGRMSRVFKAVDQQKVESRVTDPNIAIKVLTVPFVNYSDAMDV
                     LAREILSLHGSAHPNIARVMECERDGDTVFIPMDLLPGQSLLDRLEGLKTPMPKADAL
                     RIVKGITDALQFAHGKNIVHGDLKPGNVMLTGTNHAKVTDFSLARLMALSSNGPSRMT
                     RATGRMKVLPPSYASPEVLEVGRLDPRDDVFALACIAWKLLTGEHPFKGKSALAAREA
                     GMKLTCPSQLSEREFNALSHGLEFERNKRTLSPRQFYIELSGKSAGPSKAVIGAIAAL
                     VLGAVMLAAYFALNSGSDKLKQADSAAIPDEAPAVLPPGAVFRDCATCPLMKVVAPGQ
                     FMQGSAPNDAQAQPFELPQHNVSIAYPFAAGVYEVTVGQYRQFVVEANAESNGCSVYD
                     GDWQQSNSVSWKNATEGQTDSYPVSCVSWQDAKRFAAWLSARTHHIYRLPSASEWEFA
                     ARGGSVASRPWTDPSEGCKYGNMADQTAAAHYPGWTVIPCSDKYAQAAPVGQFAPNAL
                     GLYDMLGNVFEWTEDCWADTYVGAPSDGSARTDADCAQRELRGGSWFTQPDYVRVSYR
                     DRFSPDYRSTSIGFRLIREISE"
     gene            4583..4717
                     /locus_tag="VNZ06_15395"
     CDS             4583..4717
                     /locus_tag="VNZ06_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22185.1"
                     /translation="MIARENPPYTTVDQYYLFNLVRLPNGKWVYDKFGRGGNIDGGYK
                     "
     gene            4727..5827
                     /locus_tag="VNZ06_15400"
     CDS             4727..5827
                     /locus_tag="VNZ06_15400"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWW22186.1"
                     /translation="MRIRLSLIAGSVSAAWLMWAAPAASAHGDSDPVCTTAGQLPDVV
                     AARSDLQRTPDVLTERLHLADLLVNAGCFDEAIHVLEAGQASNPRSTDLQYRLTRARS
                     MMKEKEYFEGIDQAEAAARLRRNVLRCTQLSDLEACDAVLSVQPGNADIVLAKGNALS
                     KAGRLDDAVAAYTQAAQLAPGNTAITAKLQQTQAQRQASRSRCTDGTGEAALQACQSI
                     LVKGASNEFDLTLRIANLQQSTNQVPQSLDSYIAANALRPGDKSVALAILALLDSTQR
                     KDAIALAARGSALLTLGRPTEAVAPLRQAYALAPTWPDIAKQLAAAEALSKESQPHAV
                     ARAAETNSLRVSEAPAPRRTSYSNLQPASRAN"
     gene            complement(5828..6265)
                     /locus_tag="VNZ06_15405"
     CDS             complement(5828..6265)
                     /locus_tag="VNZ06_15405"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HWW22187.1"
                     /translation="MLRRQLRGPLLGLCVAGLLCSFAPSAQSDTLFEQIGGESVLKSA
                     VDEFANIILDDSRINFAFAGTNMDIFRSRLYSQLCDLSGGGCKYSGRDMRTAHARLNV
                     SSAQFNALAEDLYQAMDKVGVSYRVQNKLVALLAPMQQDIVKK"
     gene            6511..>7597
                     /gene="lnt"
                     /locus_tag="VNZ06_15410"
     CDS             6511..>7597
                     /gene="lnt"
                     /locus_tag="VNZ06_15410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HWW22188.1"
                     /translation="MTHLPISPGSAACVLLVFLAGAASALGFAPLEWWPVTLLGLALL
                     VECTWRALRLRQAFLLGLCFGTGHFLVGLNWIATAFTYQANMPAWYGWGAVALLSLYL
                     ALYVAACCGVSWLLARRRRLAFVFIFAAAWMLNEWLRATLLTGFAWDPIGVVWIGLPW
                     IARAASWIGAYGLSGVAVLLAGLFVCGLHWRWPSTLGLSAVLAAIAILTGHLTRAPQP
                     PDNPALRVQVVQPNIDQDKKYDPELAEQHERTYVGLSGAPGATPRLLLWPEGATLHYL
                     ELEPDAREALAELLGPQDLLLTGGPSATPTADRRDYVYHNSVFAMDASGVLRWRFDKA
                     HLVPFGEYLPERAILTHLGLSRLVPGDA"
BASE COUNT         1426 a         2449 c         2359 g         1363 t
ORIGIN      
        1 gcgatgtatc tatttgccgc cggcgcgaat gtcagtcatg tccaacgcca cctcgccatc
       61 gacccaggta tcacggtcgc gaatgatcgc ctgcgccggt agcgccgcgg tggctacctt
      121 gggcccttcg atctgacaca ccgctgtgcc gacggagaag atttcaatca agctcgttcc
      181 gagctcgacg atataggttt tgactccgac gacgcgatca gcgcccagcg cccgggcttc
      241 cctgtccagc cgatcaaata cctgctctcg ggcttcatac accagcgttg tcagttcgct
      301 gatctctcct ttgacgaacc ccttcagtgc ggccatgaag ccaccgacca cacccagtga
      361 atacacggag gtcgagatca gcaacttcag tggcacgtag cccagtatgg tcagactcca
      421 cagctcctcc ccggtcagat cgctggtggc gacggcacgc tggccggcga ccggcagcag
      481 cgcatgcctg gcggcggtgc cggtcatgta catctcgtgg aagcccgcaa agcgcagaat
      541 gtgtgtacgc acgcccacca cacaattggc cccggcctgc gctgcgtgcg cggccatgcg
      601 gccgagggcc tggtgacgcg tttcattgaa cacgtcgctg aactccttga tctctccgcg
      661 ccccagcgtg cgcaacgcgc ccacgatgct tcgtactgcg cccaccgagt aggcgatatt
      721 gccgaacacg aactgcagcg gttcatagcc ggcgtccatg tggcagtaga gttcctgcgc
      781 atcgcccgca ctggtgaaca gttcgcgcct gggctgcgca tgcacacccg agcccacgaa
      841 cagaaactcg gtgaatcggg acagcgctcg caggtccgag ctcacgccga cgatgccgct
      901 gacccctgcc tggcgagctt cggccaccag gcgctggaac gccgcctcgc gcccgtcgtg
      961 aatcacctgc gtcacggccg gcacttcacc gccgatgatg ccgctaacgg ccgaacgcag
     1021 tccgcccagc agcccgatgc tctggacgct attgccgacg agtaccccct gcgggtcgag
     1081 acccttcagt cgcaggcaat agatttcatt gcctgacagg ccggtcaggt gcggcgtcgc
     1141 acgcgtactc atgctggagg ctcacggttg agagtcgtca ggagtggact gtaccgctcc
     1201 ggcgcgctcg gcgcgcagcg gaaacatcag cagcaggcgc agatcgaaga tcgcatgcag
     1261 caccacgggc agcaccaggc tgccggtggt caggtagaaa ctggcaaaca ggccgcccag
     1321 gaccgccgtg gcgatgatgc cctggactcc ctgatagaaa tgcaacaggc cgaagaccac
     1381 cgaagtgagc gccgcaattg cgatcaggtt cagcggcggc agcaagacac tcaggtagta
     1441 gatcagaaag cctcggatcc acagctcttc ggaaacgccg gcgccgatgc tgaccgcggc
     1501 gtaaccggcg cgctcgcgtc gggtctcggg tacgaacagc ttgacccatg gggtctcgcg
     1561 cagcttctgc gctttggctc gcagcaggaa tgaaggaatg ataagaccga cgcacacgcc
     1621 caccagcatt gcgagcgtga acgaccagga cttgggaagc acgaggccaa gtctggcggc
     1681 cggcacgtcc cccaggcgcc agatcgccac gaccactgcg atgatgatgg cctgctcaac
     1741 gagaatcagc cgataccaac gcatccggaa gcgcggcacg ttggcccgca tctgacgcag
     1801 cgccctgcga agcgcgtagc gtccccacca cgggctgatg agtaccgtgt agaacgccag
     1861 cgcatgcgcg agccacgcgc tcacggctag cgcaacaggc ggcaggagac cctggcgatc
     1921 cgcgaaaaat ccccgatcct gaacatgacg tgacgcctct ggtatccgag gtctgcaaat
     1981 gggttcagat ccaatgctcc caccgacttc gctgccggca gctgcgtttc aaacgccttc
     2041 ctgaaggaat cgcgtttcag cacgcgctgc agctgacgat gccgtgtcgg gtagcgtccg
     2101 gcctgcaagc gctcaccagc atagtggaaa ttgatgagaa tggaggcgac ctgctgccgt
     2161 ccactatcga cgaattcctc gagccacagc gcccgggcga atggccgggc aacggaggga
     2221 tggagtgcgt gggaggcagt ccgaaccctg gggtttgacg cgacctgaca gtcaagcgcc
     2281 aagtacgtca caccgtacct gatcagagcg tgaccggctc ccaagccgac cgcttttcat
     2341 ttggcataac ctcgctttgg cgctgcgcct taactcaagg cgctttgcga tgtagcgcgc
     2401 ggatgattgt gacgcggcgc gcagtggttc tgcagcgggc cgcgatcagg catccggcca
     2461 cttggagtgg ccgtatccac gtcgggttgg gcaaggtctc acacatcaaa cagttgaata
     2521 tgttcatgct tgtaaacgca attctctctc gcggctcggc cgtgcctgga gcctgacatg
     2581 accactcccg gcaaggcgcc ccccggttca acgccagcct cggtctccga tgaggccaaa
     2641 acgatctggc tcgatctgag cacccccgtg gaggtaagcc cgcagacggt cgaccggatc
     2701 atggacaagg gttcgaccct gaatgggcgc tatcgcctgc tcgagctgat cggcgaaggc
     2761 cgcatgagcc gtgtgttcaa ggcggttgac cagcagaagg tcgagtcacg cgtcacggat
     2821 ccgaacatcg ccatcaaagt gctgaccgtt ccattcgtca actattcgga tgcgatggac
     2881 gtcctggctc gcgagatcct cagcctgcac ggttcggctc atccgaacat tgcgcgcgtg
     2941 atggaatgcg agcgcgatgg cgataccgta ttcatcccca tggacctgct gcccggccag
     3001 tcactgctcg accggctgga agggctgaag actccgatgc ccaaggccga tgcgttgcgc
     3061 atcgtcaaag gcattaccga tgcgttgcag ttcgctcacg gcaagaacat cgtccacggc
     3121 gatctcaaac ctggcaacgt catgctgacc gggacgaacc acgccaaagt taccgatttc
     3181 agcctggcac gcctgatggc gctctcgtcc aacggtcctt cgcggatgac ccgcgcaacc
     3241 gggcgcatga aagtgctgcc accctcctat gccagccccg aagtgctgga ggtgggccgc
     3301 ctggatccgc gcgacgacgt ctttgcactg gcctgcatcg cctggaagct gctgaccggc
     3361 gagcatccct tcaagggcaa gagcgcgctg gccgcgcgcg aggccggcat gaagctgacc
     3421 tgcccgagcc aactttcgga aagggaattc aatgccctga gccacggcct tgagttcgag
     3481 cgcaacaagc gcacactctc gccgcgccag ttctacatcg aactgagcgg caagagcgca
     3541 ggtccttcca aagccgtgat cggcgcgatt gccgcgctgg tgctcggggc ggtgatgctc
     3601 gccgcctact tcgcgctgaa cagcggtagc gacaagctca aacaggcgga tagcgcggcg
     3661 atcccggacg aagccccggc ggtactgccc cccggcgccg tgtttcgcga ttgcgcaacc
     3721 tgccctctca tgaaggtcgt cgcgcccggt cagttcatgc agggctcggc gccgaatgac
     3781 gcgcaggcgc aaccctttga gttgccacag cacaacgttt cgatcgccta ccccttcgct
     3841 gccggcgtct atgaggtcac ggtgggtcag taccgccagt tcgtggtgga agcgaacgct
     3901 gagagcaatg gctgttcggt gtacgacggc gactggcagc agagcaattc ggtgagctgg
     3961 aagaatgcca ccgaggggca gacggattca tatccggtga gctgcgtgtc atggcaggac
     4021 gccaagcgct tcgcggcctg gttatccgcc cgcacgcacc atatctatcg cctgcccagc
     4081 gcttcggagt gggaattcgc cgcgcgtggc ggcagcgtgg cctcacgtcc ctggaccgat
     4141 ccgtccgaag gctgcaagta cggcaacatg gcggaccaga ccgcagccgc gcactatccg
     4201 ggctggacgg tcataccctg ctcagacaag tatgcccagg ccgcgcccgt cggtcagttc
     4261 gcgcccaacg cgctgggact gtacgacatg ctcggcaacg ttttcgagtg gaccgaggac
     4321 tgctgggctg acacctatgt cggtgcgccc agcgatgggt ccgcccgcac cgacgcggac
     4381 tgcgcgcagc gcgaactgcg cggcggttcg tggttcacgc aaccggacta cgtccgcgtt
     4441 tcttatcgcg ataggttctc tcccgactat cgcagcacca gcattggatt ccgcctgata
     4501 cgggagatct ccgaatgaga gccttggcgt cattgagcaa actcgcactg ccgctaagca
     4561 gctgcgcaag gagttgcagg acatgattgc gcgcgagaat ccgccctaca cgacggttga
     4621 ccagtactac ctgttcaacc tggtgcgcct gccgaatggc aagtgggtgt acgacaagtt
     4681 cgggcgcggc ggcaatatcg acggcggtta caaataggca gttcccatgc gaatccggct
     4741 ttcattgatt gcaggcagtg tgtccgcggc atggctgatg tgggctgcgc cggcggcatc
     4801 cgcgcacggc gattccgatc cggtctgcac gaccgccggt cagctgccgg atgtcgtggc
     4861 cgcacgttcc gatctgcagc gcacgcccga tgtgctcacc gagcgcttgc acctggcgga
     4921 cctgctggtc aacgccggat gtttcgatga ggccattcac gtgctggagg caggccaggc
     4981 cagcaacccg cgcagcaccg atctgcagta tcggctgacg cgcgcgcgca gcatgatgaa
     5041 ggagaaagaa tacttcgagg gtattgatca ggcggaagcc gctgcccggc tacgccgcaa
     5101 tgtgctgcgc tgcacgcagc tgagcgacct ggaggcctgc gatgcggtgc tgagtgtgca
     5161 acccggcaac gccgatatcg tgctggccaa gggcaacgcc ctgtcaaagg cggggcgcct
     5221 cgatgatgcc gtcgccgcct atacccaggc agcgcagctc gcgccgggca acaccgcgat
     5281 taccgccaag ctgcagcaaa cccaggcgca gcgtcaggcc agccggagtc gctgcacgga
     5341 cggaactggc gaggcggcgc tgcaggcctg ccagtcgatc ctggtgaagg gtgcgtcaaa
     5401 cgagtttgat ctgacgctac gcattgcgaa cctgcagcag tcaaccaatc aggttcccca
     5461 gtcgctcgac tcctacatcg cagccaatgc attgcggccg ggcgacaagt cggtggcact
     5521 ggccatcctt gcgctgctgg atagcacgca gcgcaaagat gcgatcgcgc tggcggcgcg
     5581 cggttcggcg ctgctgacgc tgggtcgtcc taccgaggcg gtcgcgcccc tacgccaggc
     5641 ctatgcgctg gcgcccacgt ggcccgatat tgccaagcag ctcgctgcgg ccgaggcact
     5701 cagcaaggaa tcacagcccc acgcggtggc gcgtgctgcc gagaccaata gcctgagagt
     5761 cagcgaggct ccggcaccgc gtcggacctc ctactcgaac ctgcagccgg caagccgcgc
     5821 gaactgatta tttcttgacg atgtcctgtt gcatcggcgc gagcaatgcc accagcttgt
     5881 tctgcacccg gtagctgacg ccgaccttgt ccatcgcctg gtagagatct tccgccagcg
     5941 cattgaactg cgcgctgctc acgttgagtc gcgcgtgtgc ggtgcgcatg tcgcggccgg
     6001 agtacttgca gcccccgccg ctgagatcgc acaactgcga gtacaggcgg ctcctgaaga
     6061 tgtccatgtt ggtaccggcg aatgcgaaat tgatgcggct gtcgtccaga atgatgttgg
     6121 cgaactcgtc caccgccgac ttcagcaccg actcgccacc gatctgctcg aacagtgtgt
     6181 cggattgagc gctcggcgcg aagctgcaca ggaggccggc cacacacagc ccgagcagcg
     6241 gtccgcgaag ttgacgtcgc agcatgagac tctccgatca gaatccgatt tgctttgact
     6301 atacgtagca actcattgtt tggatgcgac gggattcgaa gtccccctac cggcgcgcga
     6361 tgccgatgcg cgggcgtgca atcacacggt gcaaccggcg tgcaatagac tcacccagag
     6421 cacaacgccc gccgaactga tacgaaagtg tcttgttaga attatcacgt aactcattct
     6481 ttatacttgt ctaatagtat tagtcgccaa gtgacccacc tcccaatcag tccgggcagt
     6541 gccgcctgcg tgttgctggt ctttctggcg ggcgccgcct ccgcgctcgg gttcgctccg
     6601 cttgagtggt ggccggtcac gttgctgggg ctggcgttgc tggtcgagtg cacctggcgc
     6661 gcgcttcgcc tgcgtcaggc gtttctgctg ggactgtgtt tcggaaccgg acacttcctg
     6721 gtcgggctga actggatcgc gaccgccttc acgtatcagg cgaacatgcc agcgtggtat
     6781 ggctggggcg cggtcgcgct gctgtcgttg tacctcgcgc tctatgtggc tgcctgctgc
     6841 ggcgtgagct ggttgctcgc aaggcgccga cgcctcgcgt tcgtattcat ctttgctgcg
     6901 gcgtggatgc tcaatgagtg gctgcgcgca acactgctta ccggtttcgc ctgggatccg
     6961 atcggggtgg tatggatcgg ccttccgtgg atcgcgcgcg cagcgagctg gataggagcc
     7021 tacggactgt ctggcgtggc cgtgctcctg gcaggtctct ttgtctgcgg tctgcactgg
     7081 cgctggccga gcaccctggg actgagcgcc gtgctggcag cgatcgcgat actgacggga
     7141 catctgacgc gggcgccaca gccgcccgac aatccggcgc tgcgcgtaca ggtcgtgcaa
     7201 cccaatatcg atcaggacaa gaagtacgat ccggagctgg ccgaacaaca cgaacgcacc
     7261 tatgtcggcc tgtccggcgc cccgggcgcg acgccgcgcc tgctgctatg gcccgaaggc
     7321 gccaccctgc actatcttga actcgaaccg gacgcacgag aggcgctggc agagctccta
     7381 ggaccccagg acctgctgct caccggcggt ccgtccgcga cgccgacggc ggacaggcgc
     7441 gactacgtct atcacaacag tgtgttcgcg atggatgcga gcggcgtgct gcgctggcgc
     7501 ttcgacaaag cgcatctggt gccctttgga gagtatctgc ctgaacgcgc gatcctgacg
     7561 catttgggac tctcgcgcct cgtgcccggt gatgccg
//
LOCUS       DATMRM010000506         5953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4018143, whole genome shotgun sequence.
ACCESSION   DATMRM010000506 DATMRM010000000
VERSION     DATMRM010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4018143"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /gene="queG"
                     /locus_tag="VNZ06_15415"
     CDS             <1..798
                     /gene="queG"
                     /locus_tag="VNZ06_15415"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HWW22189.1"
                     /translation="LLRERESGFISRYALGRDYHKVLRKALRGLADAVARIAGPHGYR
                     VFVDSAPVLEKALARNAGLGWIGKHTNLIARDAGSWFFLGEIYTDLPLPVDPPASEHC
                     GSCSACIPACPTGAIVAPYQLDARRCISYLTIELHGAIPEELRPALGNRIYGCDDCQI
                     VCPWNKFARDASHPDFRVRHGLDSPRLSELFGWSASQFEERMRGSAIYRIGYERWLRN
                     IAVALGNANSTPEVIAALQARRADSSALVREHVHWALRQHSQRVDTH"
     gene            complement(812..2854)
                     /locus_tag="VNZ06_15420"
     CDS             complement(812..2854)
                     /locus_tag="VNZ06_15420"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWW22190.1"
                     /translation="MQKGLALLVTMAWVAVPAIALSQRAFTPKDLVMLDRISDPNLSP
                     DGANIAYQLRQTDFAANKGVTSIWLTGPGGTRVLSGPGSTSPRWRGDGKLLYFLSSRT
                     GGSQVWRLDMSGGDAQQVTNAALDVGSFLLSPDGKHLIVSMDMFLDCDSVECTKQRMD
                     ARAARKFSGQQFEQLFVRHWDTWSDGTRSQLFVYALDEQGIAQGAPVWVSKGIQGDVP
                     SKPFGDAGEYGITPDGKDLIFTARVAGRTEAWSTNFDLYRRRIDGSGSVQNLTADNPA
                     ADTGPAISPDGKTLAYLAARRPAVESDRNAIVLKDLASGSTRELLPTWDRSADSLKWS
                     ADGKTLYALADDRGQKRLFAIDVARARVSALTDQGEVSAYDVGRKGIVVALESLGSPT
                     QLYRVATGPRLQQVTHANEDKLAGIAMAGYEQFEFPGWNNESVQGFVMKPSNYQAGKK
                     YPVAFIIHGGPEAAFGNIFHYRWNAQTYAGAGFAVVMIEFHGTPGYGQAFTDSIAGHW
                     GDRPLEDLQKGWAYALAHYPFLDGSRACALGASYGGFMIDWIAGNWSSPWKCLVSHDG
                     IFDTRVMYYSTEELWFEEYEYQGTQFAVPANYERFNPLDHVAEWKVPTLIIQGGRDFR
                     VPFEQGMGAFTALQRRGIPSEFLYFPDENHWVLKPQNSLQWHDAVFGWIKQWTDTP"
     gene            complement(2888..3739)
                     /gene="panC"
                     /locus_tag="VNZ06_15425"
     CDS             complement(2888..3739)
                     /gene="panC"
                     /locus_tag="VNZ06_15425"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005107864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HWW22191.1"
                     /translation="METVSTIEAVRARIRDWRRSRLRIAFVPTMGNLHAGHMSLLEAA
                     HSHGDRVVASIFVNPLQFGPSEDYTAYPRTLADDQKLLEQGGCDLLFAPSVNEMYPTG
                     GDQRTLVTVRGLSNVLDGEFRPGHFDGVATVVTKLFGIVAPDVAVFGEKDYQQLLVVR
                     NMTLDLALPVEIVGAPTVRAEDGLALSSRNRYLSAEERARAPAIHHSLLQAAQALSAG
                     TADYALLQQQGGQWLERSGMKLDYFSIRNASDLTAPTPDSKEFVILVAARLGRARLID
                     NMRARRA"
     gene            complement(3749..4423)
                     /locus_tag="VNZ06_15430"
     CDS             complement(3749..4423)
                     /locus_tag="VNZ06_15430"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010158266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HWW22192.1"
                     /translation="MKQQNMAPENLTHRFIVLEGPIGVGKTMLATRLASTLIGELVLE
                     RADENPFLERFYRNPRAGALPAQLYFLFQRAQQLAALKQQDLFTQMRIADYLIDKDRL
                     FARTTLDDEEYRLYEQVYAKLAIDAPKPDLVIYLQAPVDVLLSRIARRGIQYEQFIDR
                     EYLERLNDAYARFFHEYDATALLIVNAADIDPINSDQDFEELLTAIKRMRRGRLYYNP
                     LRHTEM"
     gene            complement(4398..4910)
                     /gene="folK"
                     /locus_tag="VNZ06_15435"
     CDS             complement(4398..4910)
                     /gene="folK"
                     /locus_tag="VNZ06_15435"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWW22193.1"
                     /translation="MTWFPVYVGIGSNLEQPIEQVRRALDALAGLPRTRLERRSSLYG
                     SEPIGPAKQPPYVNAVAALLTQLPVRPLFDALRDLELRLGKRAPRERWGARTIDLDLL
                     IYSSLRTQEPDLTLPHPGIVQRNFVLYPLREVAPELSVPGGGPVGMLASRVDSAGIWR
                     LNDETTKHGA"
     gene            complement(4911..>5953)
                     /locus_tag="VNZ06_15440"
     CDS             complement(4911..>5953)
                     /locus_tag="VNZ06_15440"
                     /inference="COORDINATES: protein motif:HMM:NF024040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HWW22194.1"
                     /translation="DAPAANNDRVLDTHGRLLRDNVYGTLEEDVWRRDFTVNALYYNI
                     ADFSLWDFVGGARDVAARRLRLIGDPEIRYREDPVRMLRGARFEAKLGFTIEESTAAP
                     IAGLRHLLAGVPAPRLFDETLKLFLTGHGERSLAVMRQRQLLEVLLPSVERYLTTHAG
                     NLVEQLLVQGLRNTDARVSADKPVTPTFLFALLLYGPIAQYIESLPPQRWHEAGAILE
                     ACDRALREASRRVTIPRRVALGVREMYGLQPRLEQPRGKRALRLLEQPRFRAGFDLLS
                     LRAQFGLASAAMADWWTRLQETAPEERAKALDALPATAATPPAGSAAHNTNSGGVRTR
                     RRRRRRRSPGPA"
BASE COUNT         1113 a         1921 c         1841 g         1078 t
ORIGIN      
        1 ctattgcgcg agcgcgagag cgggttcatt tcgcgctacg ctctcggtcg cgactatcac
       61 aaagtactca ggaaagcgct gcgcggactg gcggacgcgg tcgcgcgcat tgccggtccg
      121 cacggctatc gcgtgttcgt cgacagcgca ccggtgctgg aaaaggccct tgcacgcaac
      181 gccggcctcg gctggatcgg caagcacacc aatctgatcg cgcgcgatgc cggctcctgg
      241 ttcttcctgg gcgagatcta tacggatctg ccgctgccgg tggatccgcc ggccagtgag
      301 cactgcggca gctgctcggc gtgcatcccg gcgtgcccga ccggcgcgat cgtggcaccg
      361 tatcagttgg acgcgcggcg ttgcatttcg tatctgacga tcgaactgca cggcgcgatt
      421 ccggaagaac tgcgcccggc gctcggcaat cgaatctacg gctgtgacga ctgtcagatc
      481 gtttgtccct ggaacaaatt cgcgcgcgac gcctcgcatc cggactttcg ggttcggcat
      541 ggcttggatt cgccacgctt gagcgagctg tttggctgga gcgcgtcgca attcgaggag
      601 cgcatgcgcg gctcggcgat ctatcgcatc ggctatgagc gctggctgcg aaacatcgct
      661 gtcgcgctcg gcaacgccaa ttcgaccccc gaggtcatcg cagcgctgca ggcgcggcgt
      721 gctgattcgt cagcgctggt acgcgaacac gtgcactggg cactgcgcca gcactcgcag
      781 cgcgtggaca cacactaggg cactttaagc actagggcgt atcggtccat tgcttgatcc
      841 agccgaacac tgcgtcgtgc cactgcaggc tgttctgcgg tttcagcacc cagtgattct
      901 catccgggaa gtacaaaaac tcgctcggga tccctcgtcg ctgcaacgca gtgaacgcgc
      961 ccatgccctg ttcgaacggc acgcggaagt ccctgccgcc ctgaatgatg agcgtcggca
     1021 ccttccactc ggccacgtga tcgagcggat tgaagcgctc gtagttcgcc ggaactgcga
     1081 actgggtgcc ctgatactcg tactcctcga accacaactc ctcggtgctg tagtacatca
     1141 cgcgggtatc gaatatgccg tcgtgactga ccaggcactt ccacggactc gaccagttgc
     1201 cggcgatcca gtcgatcatg aaaccgccgt agctcgcgcc caacgcgcag gcgcgtgagc
     1261 catcgagaaa cggatagtgc gccagcgcat acgcccagcc cttctgcagg tcttccaacg
     1321 gccggtcacc ccagtgtccg gcaatcgaat cggtgaaggc ctgaccgtag ccgggcgtac
     1381 cgtgaaactc gatcatgacc accgcgaagc cggccccagc gtaggtttgc gcgttccagc
     1441 gatagtgaaa gatattgccg aatgcggctt ccgggccacc atgaatgatg aatgcgaccg
     1501 gatatttctt tcccgcttga tagttggatg gcttcatcac gaagccctga accgactcat
     1561 tgttccagcc ggggaattca aactgctcgt aaccggccat cgcgatgccc gcaagtttgt
     1621 cctcgttggc gtgcgtgacc tgctgcaatc tgggaccggt agcgacgcga taaagttgtg
     1681 tcggactgcc cagtgactcg agagcaacga cgatgccctt gcggccgaca tcgtaagcgc
     1741 tgacttcccc ctggtcggtg agtgcactga cccgagcacg ggcgacgtcg atcgcgaaca
     1801 ggcgcttctg gcccctgtca tcggccagcg catacagcgt cttgccatcg gccgaccatt
     1861 tgagactgtc ggctgagcga tcccatgtcg gcagcagctc gcgggtgctg ccgctggcga
     1921 gatccttcaa cacgattgcg ttgcggtcag actcgaccgc cgggcgtctg gcagccagat
     1981 aggccagcgt tttgccatcc ggagatatcg ccggaccggt gtcggcggcc gggttgtcgg
     2041 cagtcaggtt ctgcacactg cctgaaccgt caatgcggcg acgatacaga tcaaagttcg
     2101 tgctccaggc ctcagtgcga cctgcgacgc gggcggtgaa gatcaggtcc ttgccgtcag
     2161 gcgtgatgcc gtactcgccg gcatcgccaa agggcttcga gggcacgtcc ccctggatac
     2221 ccttgctgac ccacaccggt gcgccctgcg cgatgccttg ttcatccagc gcatagacaa
     2281 agagctgcga gcgcgtgcca tccgaccagg tgtcccaatg ccgcacgaac agctgttcaa
     2341 actgctgccc cgagaatttg cgcgccgcgc gcgcgtccat gcgctgcttg gtgcattcaa
     2401 cggaatcgca gtccaggaac atgtccatcg agacgatcag atgcttgccg tcgggcgaca
     2461 gcagaaaact gccgacgtcc agcgcagcat tcgtcacctg ctgagcatca ccgccgctca
     2521 tgtccagtcg ccagacctgt gagccgccgg tgcgactgga gaggaaatag agcagtttgc
     2581 cgtcgccgcg ccagcgcggt gaggtactgc ccggtccgct caacacccgc gtgccgccgg
     2641 gcccggtcaa ccaaatgctg gtaacaccct tgttcgcagc gaaatcggtt tgccgcagct
     2701 gataggcgat attcgcgccg tcgggcgaaa gattcggatc ggagatgcga tcgagcatca
     2761 ccagatcctt gggagtgaat gcgcgttgcg acagcgcgat ggccggtacc gcgacccatg
     2821 ccatcgtgac cagaagagcc aggcctttct gcatgattgt tctccgtcag atggcggtta
     2881 cgcgcggtta cgcgcgccgt gcgcgcatat tgtcgatgag ccgcgcacgc cccagacgcg
     2941 ccgccaccag gatcacgaac tccttcgagt caggcgttgg cgccgtcagg tcagaggcgt
     3001 tgcggatcga gaagtagtcc agcttcatgc cagagcgctc aagccactgc ccgccctgct
     3061 gctgcagcaa agcgtagtca gccgtaccgg ctgacagagc ttgcgccgcc tgcagcaatg
     3121 aatgatggat cgcgggcgcg cgtgcccgct cttcggcgct gagatagcga ttgcgcgagc
     3181 tgagggccag gccgtcctcg gctcgcacgg ttggcgcgcc gacgatctcg accggcaaag
     3241 ccagatcgag ggtcatgttg cgcaccacca gcagctgctg gtagtccttc tcgccgaaca
     3301 ccgcgacgtc aggcgcgacg ataccgaaca gcttggtcac gacggtcgca acaccatcga
     3361 agtgaccggg tcgaaattcg ccgtccagca cgttcgacag accgcgcacc gtgaccagcg
     3421 tgcgctggtc gccgccggtc ggatacatct cattgacgct cggagcaaac agcagatcgc
     3481 accccccctg ctccagtagt ttctgatcgt ctgcgagcgt acgcggatag gcggtgtagt
     3541 cctcgctggg accaaactgc agcggattga cgaaaatgct cgcgaccacg cgatcgccat
     3601 gactgtgcgc ggcctcgagc agactcatat ggccggcatg cagattcccc attgtgggca
     3661 cgaacgcaat gcgcaatcgc gaccgacgcc aatcgcggat gcgcgcgcgc accgcctcga
     3721 tggtgctgac cgtttccata gcgttgctct acatctccgt atggcgcaag gggttgtagt
     3781 acagccggcc gcggcgcata cgcttgatgg cggtcagcaa ttcctcgaag tcctgatcgc
     3841 tgttgatcgg atcgatatcg gctgcgttga cgatcaacaa tgcggtcgcg tcgtactcgt
     3901 ggaaaaagcg cgcataggcg tcattgagcc gttcgaggta ttcgcggtcg atgaattgct
     3961 cgtactggat gccacgtcgg gcgatgcgcg aaagcagcac atccactggc gcctgcagat
     4021 agatgaccag atccggcttg ggcgcatcga tcgcgagctt ggcatacacc tgctcgtaga
     4081 gacggtactc ctcatcgtca agcgtcgtcc tggcgaacag ccgatccttg tcgatcagat
     4141 agtcggcaat gcgcatctgc gtgaacagat cctgctgttt cagggcggcc agctgttgcg
     4201 cgcgctgaaa caggaagtac aactgcgcgg gcagcgcgcc ggcccgcggg ttgcggtaga
     4261 aacgctccag aaatggattc tcgtcggccc gttccagcac caactccccg atcagcgtcg
     4321 aggcgaggcg cgtggcgagc atggttttgc ctactccgat cgggccttcc agcacaatga
     4381 agcggtgcgt cagattttca ggcgccatgt tttgttgttt catcgttcag acgccagatg
     4441 cccgcggagt cgacacgcga agcgagcatc cccacgggcc cgccgcccgg aacactcagc
     4501 tccggcgcca cctcgcgcag aggatacaga acgaagttac gctgcactat cccgggatgt
     4561 ggcagcgtca gatcaggctc ctgcgttcgt agcgagctgt agatcagcag gtccagatcg
     4621 atcgtccgcg caccccatcg ctcgcgcggc gctcgcttac cgagccgaag ctccaagtcg
     4681 cgcagagcgt cgaacagcgg acgaaccggg agctgcgtca agagtgccgc cacggcgttg
     4741 acgtagggcg gctgcttggc cggaccgatg ggctcggatc cgtacagcga cgagcggcgc
     4801 tcgagccggg tgcgcggcaa gcccgccagg gcgtccagcg cacgccgcac ctgttcgatc
     4861 ggctgttcca ggttgctgcc gatgccgacg tacaccggaa accacgtcat tcaggccgga
     4921 cccggagatc gtcgccgtcg tcgccggcgg cgcgtgcgaa caccccctga gttcgtgttg
     4981 tgcgcggcgc tgccagcagg cggcgtcgca gccgtggccg gcagcgcatc cagtgccttg
     5041 gcgcgctcct ccggtgcagt ctcctgtaac cgagtccacc agtcggccat ggctgcagag
     5101 gccaggccga actgcgcgcg caacgacagc aggtcgaagc cggcgcgaaa gcgcggttgc
     5161 tccagcaatc gcaaggcgcg tttgccgcgc ggctgctcaa gccgcggttg caggccgtac
     5221 atctcacgta cgcccagtgc cacacgcctt ggaatggtta cgcgacgcga tgcttcgcgc
     5281 agcgcgcgat cgcacgcttc cagaatggcg ccggcctcgt gccagcgctg tggcggcagg
     5341 gattcgatgt attgcgcgat cggcccgtag agcaacagcg caaacagaaa ggtcggtgtg
     5401 acgggcttgt cggcactcac gcgcgcgtcg gtgttgcgca gtccctgcac cagcaattgc
     5461 tccaccagat tgccggcgtg agtcgtcagg tatcgctcga cgctgggcag cagcacttcc
     5521 agcaactgac gctgacgcat gaccgccaga ctgcgctcac cgtgcccggt cagaaacagc
     5581 ttcagcgtct catcgaacaa gcgcggcgcc gggacacccg cgagcagatg tcgcaggccg
     5641 gcgatcggtg ccgcggtgct ctcctcgatc gtgaaaccga gcttcgcttc aaagcgtgct
     5701 ccgcgcagca tgcggaccgg atcctcgcgg tagcggatct ccgggtcgcc aatcagacgc
     5761 aaccggcgag ccgccacgtc ccgcgcgcca cccacaaaat cccatagcga gaaatcagca
     5821 atgttgtagt aaagcgcatt gacggtgaaa tcgcgccgcc agacatcctc ttccagcgtg
     5881 ccataaacgt tgtcgcgcag caggcggccg tgcgtgtcca gcacgcgatc attgttcgcc
     5941 gccggcgcat ccg
//
LOCUS       DATMRM010000507        10442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_4019139, whole genome shotgun sequence.
ACCESSION   DATMRM010000507 DATMRM010000000
VERSION     DATMRM010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10442
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4019139"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..806
                     /gene="map"
                     /locus_tag="VNZ06_15445"
     CDS             18..806
                     /gene="map"
                     /locus_tag="VNZ06_15445"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005170579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWW22195.1"
                     /translation="MLRTTQISLKTPDEQQKLRVAGILAADVLDMIGDHVKPGISTDE
                     LDQICNDYIVKVQKTVPANVGYRGFPKTVCTSVNHVVCHGIPNDRKLKNGDIVNVDVA
                     IIKDGYHGDTSRMYFVGKPAPFAQRLTQVCKDAMWRAIELVRPGAHLGDIGHAIQSMA
                     EAEGFSVVREYCGHGIGRVYHEDPQILHYGEAGTGMEMRAGMVFTIEPMINAGKRHVR
                     VLEDGWTVVTKDHSLSAQWEHMILVTPTGHEVLTLSPRAAAAGP"
     gene            817..3516
                     /gene="glnD"
                     /locus_tag="VNZ06_15450"
     CDS             817..3516
                     /gene="glnD"
                     /locus_tag="VNZ06_15450"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HWW22196.1"
                     /translation="MAVPDTAALEDIAPHTPVPLDWPLLNRLPTLLAESHCEGEACRK
                     LLTQAQEELRTRFFEDEPVEGLVRARARFIDVLLGTLWAQRLDPELAANLALVAVGGY
                     GRGELHPHSDVDLLVLVPAPLNEQQRAKVEQLVSFLWDIGLEVGHSVRTIAECAEESA
                     ADVGVMTTLLEARQLAGSEPQVAAMRVALASDRVWPVREFFEAKVREQQERRLKANDT
                     AYNLEPNVKTGPGGLRDIQTIAWVAKRHFGAESLDELVTHGFLTPSELRRLKQAQALL
                     WKVRFGLHVLSGRHEDRLLFDYQIKLAQAFGYEDGSYTLAVEQLMQRYYRTIIDVTLL
                     NELLLQLFREAILTDDQPPLPLNPRFRVRNDYLEAVNDDVFGRYPSALLEIFALLQQN
                     PQIRGVRAGTIRAVVRNLWLIDEEFRQNPRNHRLFLEILRAPRGVTHELRRMNDYGVL
                     GRYIPAFGRVIGRMQYDLFHVYTVDAHTLFVVSNLRRLAMSEFDHELPLVSAIMQLLP
                     KPEIAYLGALFHDIAKGRGGDHSDLGAVDAEAFCLEQGMSRYDARLVAWLVKNHLLLS
                     MTAQKQDIADPQVVSEFARKVGDEVHLDYLYVLTCADVRGTNPKLWNSWKASLFNEFY
                     ERVKQALRRGLETPIDQDELVREVQDSARMMLRAGGVSPDTIERTWARLPTPYFMRHT
                     PEEITRHTRALAAADGNADEPLVSVEPQSERGTTSVMIYAPHRRHGFARATAVLDQLG
                     LNILDARITPVGNGFSLDSYHVLEEDGSPITDAERRMEIEQALWGSLQRPEEAPLAVS
                     RRASRQVRVFKTPTQIAISIDERNRRSVLELTASDRPGLLCDVGKVLWEERIDLHAAK
                     ISTVGARAEDVFYLTDRAQQPLDEAAAERLTTRLTDALDRRAV"
     gene            3513..4709
                     /gene="dapC"
                     /locus_tag="VNZ06_15455"
     CDS             3513..4709
                     /gene="dapC"
                     /locus_tag="VNZ06_15455"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HWW22197.1"
                     /translation="MNRGLSRLKAYPFERLAALLSDARAPEGIPAIALSIGEPRHEPP
                     AFVLDVLRANLQQLGTYPATRGLPRLRSAMAQALQQRLLPAGALLDPQSMVLPVNGTR
                     EALFAFAQTVIDSAGHALVAMPNPFYQIYEGAALLAGAEPYFLNTTEATGYLPDLDAV
                     PQSIWSRCRLLFLCSPGNPTGAVLSLEYLRRALQLAERYNFVIAADECYSDLYLDESQ
                     PAPSLLRAAYENGNTRFTGCVVFHSLSKRSSLPGLRSGLVAGDPELIAAFLRYRTYHG
                     CAMPMPTQLASVAAWSDEKHVTANRRLYQQKFDRVLPILQDVLPLSRPAGGFYLWPRV
                     SDDDAGFARALYERTHLTILPGSYLAREHAGENPGRGRVRISLVPTIEQCVEAAQRLR
                     DFIRAR"
     gene            complement(4712..6502)
                     /locus_tag="VNZ06_15460"
     CDS             complement(4712..6502)
                     /locus_tag="VNZ06_15460"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWW22198.1"
                     /translation="MSKPTLDLAMVGNCQIASLIDRQATHVWTCWPRLDGDPVFCDLL
                     AGSDAPQRAGLWSIELIDLASSTQVYERNTAIVETTLTDRDGASLVVTDFAPRFRQYE
                     RNYRPAMLVRIVRRLSGRPRLRMKLLPAERYGERARSTTVGSHHIRYSGRDVDLRMTT
                     NASVAVIQEQRVFVLDDSLAFILGPDETVHESVPMLAQRLLDATREYWNDWVRSLAIP
                     FEWQEAVIRAAITLKLCSYEDTGAIIAAITTSIPESADSGRNWDYRFCWIRDTYFTVQ
                     ALNRLGATRTMEGYLRFIQNLTATPPLQLQPCYGIDGELHLDEKVAPQLKGYRGMGPV
                     RVGNAAFSQVQNDVYGAAILSVTQSFFDERLVRRGTEALFNALEPLGERALAMFTQPD
                     AGIWEFRGRTGVHTFSSAVCWAAADRLARIAARLGLVDRERYWRPHAQKMAKRIREEG
                     YSRSLTSFTSTLKGDSVDASLLMLGELGVVDWKDPMFVSTVDRIGKTLKKGKFLARYA
                     DPDDFGVPEVAFTVCTFWYISALWNVGRCDEARELFEEMLRQRNSLGLMSEDLAPGSG
                     ELWGNYPQTYSMVGIINCAMRLSRSWEEAM"
     gene            complement(6495..7259)
                     /gene="otsB"
                     /locus_tag="VNZ06_15465"
     CDS             complement(6495..7259)
                     /gene="otsB"
                     /locus_tag="VNZ06_15465"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HWW22199.1"
                     /translation="MQTLFKKTAARDILTGHALFLDIDGTLLELAPSPEAVEVPHTLV
                     PVLQQLADRLGGAIAFVSGRTIEAIDALFNPLKLPAVGVHGGEVRTPDGLILADSGLV
                     DEIQATEPLLREAIGGMRGVVLENKHSAIALHYRSVPERGREVLKVAELVVGGMGSQF
                     AVLMGKCVVEIRPRHQTKGIAIDQLMRMAPFRGRTPIFAGDDCTDEDAFEVVNRLGGV
                     SVHVGSSSGTQAHHRLASPDQLRGWLQQLSDNRADE"
     gene            7378..8181
                     /gene="dapD"
                     /locus_tag="VNZ06_15470"
     CDS             7378..8181
                     /gene="dapD"
                     /locus_tag="VNZ06_15470"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:TIGR00965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HWW22200.1"
                     /translation="MEAAYERRQELSAGTVDAETRAAIIEAVAGLDRGQFRVAEKIDG
                     RWQVHTWLKQAVLLYFRIHDNQLVEGGFTRFYDKVPLKYAQHDAARFAAEAVRVVPHA
                     IVRHGAYVAANAVLMPCFVNIGAYVGAGTMVDTWATVASCAQIGSNVHLSGGVGIGGV
                     LEPLQATPTIIEDNCFIGARSEIVEGVIVGAGSAISMGVFIGANTPIYDRTTGETFRG
                     AVPPGSVVMAGSMPSKDATHSLNCAVIVKRVDAQTRAKTSLNELLRPSD"
     gene            complement(8189..9106)
                     /gene="pstB"
                     /locus_tag="VNZ06_15475"
     CDS             complement(8189..9106)
                     /gene="pstB"
                     /locus_tag="VNZ06_15475"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HWW22201.1"
                     /translation="MATLVKGMPSERESSGFLPASDIDTGVIPIRTSGRGMLQVAVDE
                     ARRSAALKAPIKAKTEHLNFFYGAFKALKDLNMEIREKQVTALIGPSGCGKSTFLRCF
                     NRMHDLTPGCRYEGNITLFPENMDLVGRDVDPIEVRMRIGMVFQKPNPFPKSIFENIA
                     YGLRIRGVRSQIRIREEVEKALRGSALWDEVKDRLSSSALSLSGGQQQRLCIARALAT
                     SPEILLFDEPTSALDPIATARIEELIAALREQVTVLIVTHNMQQAARVSDYTAFMYLG
                     ELIEFDETSKIFTNPGKKATEDYITGRYG"
     gene            complement(9121..9843)
                     /gene="phoU"
                     /locus_tag="VNZ06_15480"
     CDS             complement(9121..9843)
                     /gene="phoU"
                     /locus_tag="VNZ06_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWW22202.1"
                     /translation="MSTEGVSHHISSRYNEDLERLRSSVLEMGGLVERQLIQAIGGIT
                     EPDAKVMLRVAQEELRVNQLERSIDEDCSRILATRGPAASDLRLIISILKTITDLERI
                     GDEGEKVAAIAARLAARERPNNRYRELRNLGELVIAMVHDTLDAFARYDTDLALQVLR
                     RDRIVDEEYEAIHRQNITFMMEDPRSIGRALDVMWVVRALERIGDHAKNICEYLIYLV
                     HGKDVRHTKLEEIEKELHAPKA"
     gene            10227..>10442
                     /locus_tag="VNZ06_15485"
     CDS             10227..>10442
                     /locus_tag="VNZ06_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HWW22203.1"
                     /translation="MSATLELAHELMARASVTPADEGCQELMAARLSKLGFKVERLRY
                     GSVDNLWARRGGNGGPVLCFAGHTDVVP"
BASE COUNT         1913 a         3361 c         3214 g         1954 t
ORIGIN      
        1 agcgaacctg agactccatg ttacgtacca cgcagatttc cttgaaaacc ccggatgagc
       61 agcagaaatt gcgggtcgcc ggaatcctgg ccgccgacgt gctcgacatg atcggggatc
      121 acgtgaagcc cgggatcagc accgacgagc tcgaccagat ctgcaatgac tacattgtca
      181 aggtgcagaa gaccgttccg gcgaacgttg gctaccgcgg ctttccgaag accgtctgca
      241 cctcggtcaa tcacgtggtc tgccacggga tcccgaacga ccgcaagctc aagaacggcg
      301 acatcgtcaa cgtcgatgtg gctatcatca aggacggcta ccacggcgac accagccgca
      361 tgtacttcgt aggcaaaccg gccccgtttg cccagcgact gacacaggtg tgcaaagacg
      421 cgatgtggcg tgcgatcgag ctggtgcgtc ccggcgcgca tctgggcgac atcggccatg
      481 ccatccagtc gatggccgag gctgagggct tctcggtggt gcgcgaatac tgcggtcacg
      541 gtatcggtcg cgtctaccat gaagacccgc agatcctgca ctatggcgag gctggaactg
      601 gaatggagat gcgcgcgggg atggtgttca cgatcgaacc gatgatcaat gcaggcaaac
      661 gtcacgtgcg ggtgctggaa gatggctgga ccgtggtcac caaggaccac tccctttcgg
      721 cgcagtggga acacatgatc ctggtcacgc cgaccggaca tgaagtgctc accttgagcc
      781 cgcgggccgc agcagcaggt ccctaaagga ggttccttgg cagttcccga tacggcagca
      841 ctcgaggaca ttgccccgca cacaccggta ccgctggatt ggccgcttct caaccgcctg
      901 ccgactctgc tggcggagtc gcactgcgaa ggggaggctt gccgcaagct cctgacgcag
      961 gcccaggaag aactgcgcac gcgattcttc gaggatgagc cggtcgaggg cctggtgcgc
     1021 gctcgcgcgc gcttcatcga tgtgctgctc ggtactttgt gggcgcagcg actggatccc
     1081 gaactggctg ccaacctggc actggtcgcc gtgggcggct atgggcgcgg tgagctgcat
     1141 ccgcattccg acgtggacct gctggtgctg gtgcccgccc cgctgaacga acaacagcgc
     1201 gcgaaggtcg agcaactggt ctccttcctc tgggacatcg gcctcgaagt cggccacagc
     1261 gtgcgcacga tcgccgaatg cgctgaggag agtgccgccg acgtcggggt catgacgacg
     1321 ctgctggagg cccgccagct cgccggctca gagccacagg tcgcagcgat gcgcgttgca
     1381 ctcgcttccg atcgggtctg gcccgtgcgg gagttcttcg aagctaaggt ccgcgagcag
     1441 caggagcggc ggctcaaggc caacgacacc gcttacaacc tcgagcccaa cgtcaagacc
     1501 gggcccggcg ggctgcgcga cattcaaacc atcgcctggg tcgccaagcg ccacttcggc
     1561 gccgaatcac tcgatgaact ggtcacgcac ggctttctga cgccctcgga attacgccgc
     1621 ctgaaacagg cgcaggcgct gctgtggaaa gttcgcttcg gcctgcatgt gctgagcggc
     1681 cgccacgagg accgcctgct gttcgattat cagatcaagc tggcgcaggc attcggctac
     1741 gaagatggct cgtacacgct ggcggtcgag cagctcatgc agcgctacta ccgcaccatc
     1801 atcgatgtca cgttgctgaa cgaattgctg ctgcaattgt ttcgcgaagc cattctgacc
     1861 gacgatcagc cgccgctgcc gctcaacccg cgcttccggg tgcgcaacga ctacctggaa
     1921 gcggtcaacg acgatgtctt tggccgctac ccttccgcgc tgctcgagat attcgcgctg
     1981 ctgcagcaga atccgcagat ccgcggcgtg cgcgccggca cgatccgcgc ggtggtccgc
     2041 aatctgtggt tgatcgacga ggagttccgc cagaacccgc gcaatcaccg cctgttcctg
     2101 gaaatcctgc gcgcgccgcg cggcgtcacg cacgaactgc gccgtatgaa cgactacggc
     2161 gtgctcggac gttatatccc ggccttcggc cgcgtgatcg gccgcatgca atacgatctg
     2221 ttccacgtct acacggtgga tgctcacacg ctgttcgtgg tgtcgaacct gcgccgtctg
     2281 gcgatgagcg agttcgatca tgagttgcct ctggtctcgg cgatcatgca gctgctgccg
     2341 aaaccggaga ttgcatacct gggtgcgctg tttcacgaca tcgccaaagg acgtggcggc
     2401 gatcactccg atctgggtgc cgtggacgcc gaagcgttct gtctggagca gggcatgtcg
     2461 cgttacgacg cacgcctggt tgcctggctc gtcaagaacc atttgttgct gtccatgacc
     2521 gcacagaagc aggacatcgc cgatccgcag gtcgtcagcg agttcgcccg caaagtcggc
     2581 gatgaagtgc acctcgacta cctgtacgtg ctcacctgcg cggacgtgcg cggcaccaac
     2641 ccgaagctgt ggaactcgtg gaaggcgtcg ctgttcaatg aattctacga gcgcgtcaaa
     2701 caggcgctgc ggcgaggact ggaaaccccg atcgatcagg acgaactggt gcgtgaggtt
     2761 caggattccg cgcgcatgat gttgcgtgcc ggcggtgtct cacccgatac gatcgagcgc
     2821 acctgggcgc gcttgccgac gccgtacttc atgcggcaca cgccagagga gatcacgcgc
     2881 cacacgcgtg cgctggcggc ggccgacggc aatgcggacg agccgctggt gtcggtcgaa
     2941 ccgcagagcg agcgtggcac cacctcggtg atgatctacg cgccacatcg tcgtcatggc
     3001 ttcgcgcgcg ctaccgcggt gctcgatcag ctgggtctga acattctgga tgcccgcatc
     3061 actccggtgg gtaacggttt ttcgctcgat tcgtatcacg tgctcgagga agacggttct
     3121 ccgatcaccg atgctgagag gcgcatggaa atcgagcagg cgctgtgggg ttcgctgcag
     3181 cgaccggaag aagccccgct cgcggtctcc aggcgggcca gtcgccaggt acgggtgttc
     3241 aagacaccca ctcagatcgc gatcagtatc gatgagcgca atcgccgctc agtgctggaa
     3301 ctgactgcga gtgatcgtcc cggactgctg tgtgacgtcg gcaaggttct gtgggaagaa
     3361 agaatcgatc tgcacgctgc caagatcagt acggtgggcg cgcgcgccga agacgtgttc
     3421 tacctgaccg atcgtgcgca acagccgctc gatgaggccg ccgccgaacg tttgaccact
     3481 cgcctgaccg acgcgctcga ccgccgcgcc gtatgaaccg cggcctgtcg cggctcaagg
     3541 cgtatccgtt cgaacgccta gctgcgctgc tgtccgatgc gcgtgccccg gagggtatcc
     3601 ctgccatcgc tctttcgatc ggcgagccgc gccacgagcc cccggccttc gtgctcgacg
     3661 tgctacgggc aaacctgcag caactcggca cctatccggc cacccgcgga ctgccgaggc
     3721 tgcgttcagc gatggcccag gcactgcagc agcgcttgtt gcccgccggt gctctacttg
     3781 atccgcagag catggtgctg ccggtaaacg gcacgcgcga agcgctgttc gccttcgccc
     3841 agaccgtgat cgattccgcc gggcacgcgc tcgtggccat gcccaatccg ttctatcaga
     3901 tctacgaggg ggcggcgctg ctggccggtg ccgagcccta ttttctgaat acgaccgagg
     3961 ccacgggcta ccttccggat ctggacgcgg taccgcagtc gatatggagc cggtgtcggt
     4021 tgctgtttct gtgctcaccg ggcaatccga ccggtgctgt gctgtcactc gaatacctgc
     4081 gccgggcgct gcaactggcc gagcgctaca acttcgtgat cgctgcggac gagtgctatt
     4141 cggacctgta cctggatgaa tcgcagccgg caccgagcct gctgcgcgcc gcctatgaaa
     4201 acggcaacac gcgcttcact ggctgcgtgg tgtttcacag cctgtcgaaa cgttcgagcc
     4261 tgccgggcct gcgctcgggt ctggtggctg gcgaccctga gctgatcgcg gctttcctgc
     4321 gctatcgcac ctatcacggc tgcgcgatgc cgatgccgac acagcttgcc agcgtcgcgg
     4381 cgtggtcgga tgagaaacat gtaacggcca atcgccgtct gtatcaacag aagttcgatc
     4441 gggtgctgcc gatcctgcag gacgttctgc cgctgtcgcg gcccgctggc gggttttatc
     4501 tgtggccgcg tgtcagcgat gacgatgccg gcttcgcgcg cgctctgtat gaacgcacgc
     4561 atctgacgat tctgccggga agctacctgg cgcgcgaaca cgcgggcgag aacccggggc
     4621 gcggccgggt tcgcatctcg ctcgtaccca cgatcgagca gtgtgtcgag gccgcgcagc
     4681 gattgcgcga cttcattcgc gcccgctagc cttacatcgc ctcttcccag gaccgggaca
     4741 ggcgcatcgc gcagttgatg atccccacca tgctgtaggt ctgaggatag ttgccccaca
     4801 gctccccgct gccaggtgcc agatcctccg acatcaaccc gaggctgttt cgttggcgca
     4861 gcatttcttc gaacagttcg cgcgcctcat cgcaacgtcc gacgttccac agggcgctga
     4921 tgtaccagaa ggtacacacc gtgaaggcta cttccggtac gccgaaatca tccggatcgg
     4981 catagcgcgc cagaaacttg cccttcttga gcgtcttgcc gatgcgatcc acggtcgaaa
     5041 cgaacatcgg atccttccag tcaaccactc cgagctcacc cagcatcagc agactggcat
     5101 cgacgctgtc gcccttgagc gtgctcgtga aggaggtgag cgacctggag tagccttcct
     5161 cgcggatgcg cttggccatt ttctgcgcat gcggccgcca atatctctcg cgatccacca
     5221 acccaagccg tgcggcgatg cgtgccagtc gatcagccgc cgcccagcag accgcagacg
     5281 agaaggtgtg cacccctgtg cgcccgcgaa attcccagat accggcatcc ggctgggtga
     5341 acatggcgag cgcgcgctcg cccagcggtt ccagcgcatt gaacagcgcc tcggtaccac
     5401 gtctcaccaa gcgctcatcg aagaagctct gggtgaccga gaggatcgcg gcgccgtaaa
     5461 cgtcgttctg cacctgcgaa aaggcggcgt tgccgacccg taccgggccc atgccgcgat
     5521 accccttcag ctgcggcgcg actttctcat ccaggtgcag ttcgccgtcg atgccgtagc
     5581 acggctgcag ctgcagggga ggcgtagcgg tgagattctg aatgaacctc aggtacccct
     5641 ccatcgtccg cgtggctccc agccgattga gcgcctgcac cgtgaaatag gtatcgcgaa
     5701 tccagcagaa gcgatagtcc cagttgcggc cactgtcggc gctctccggg atcgaggtgg
     5761 tgatcgctgc gatgatcgcc ccggtatcct cgtagctgca gagcttgagg gtgatcgccg
     5821 cacgaatcac ggcttcctgc cactcaaacg gtatggcgag actgcgcacc cagtcgttcc
     5881 agtactcgcg cgtcgcatcg agcagccgct gcgccagcat cggcaccgac tcatgcactg
     5941 tctcgtccgg ccccaggatg aatgccagtg agtcgtccag cacgaacaca cgctgctcct
     6001 gaatcaccgc gacactagcg ttggtagtca ttcgcagatc gacatcgcgc cccgaataac
     6061 gaatgtgatg cgagccgacc gtggtactgc gcgcgcgttc gccgtagcgt tccgccggca
     6121 gcagtttcat gcgcagacgc ggacggccgg acagccggcg cacaatgcgc accaacatcg
     6181 ccgggcggta gttacgctca tactgtcgaa agcgcggagc gaagtccgtg acgaccaacg
     6241 aggcgccatc cctgtcggtc aatgtggtct cgacgatcgc ggtattgcgc tcatagacct
     6301 gcgtcgaact ggcgagatcg atcagctcaa tggaccacaa tcccgcgcgc tgcggtgcat
     6361 ccgaaccggc gagcagatcg cagaacaccg ggtcgccgtc cagacgcggc cagcaggtcc
     6421 acacatgggt ggcttggcga tcaatcagcg aggcgatttg gcagttgcca accatcgcca
     6481 gatccaatgt cggtttactc atccgcccta ttatcactca gttgctgcaa ccagcctcgc
     6541 aactgatcgg gactcgccag gcgatgatgg gcctgggtgc cggaggaact gccgacgtgc
     6601 acactgaccc cgcccagtcg attcaccact tcgaaagcat cctcatcggt gcaatcgtcg
     6661 ccagcgaaaa tcggtgttcg cccgcgaaac ggcgccatcc gcatgagctg gtcgattgcg
     6721 atacccttgg tctggtgtcg cggccggatt tccaccacgc actttcccat cagcaccgca
     6781 aactgactgc ccatgccccc cacgaccagc tccgcgactt tgagcacttc gcgtccgcgc
     6841 tcggggacac tgcgatagtg aagcgcgatg gcactgtgtt tgttctccag caccacgcct
     6901 cgcatgccgc cgatcgcctc gcgcaacagg ggttccgtgg cttggatctc atcgaccaaa
     6961 ccgctatcgg ccaggatcag gccatccggc gttcgcacct ccccgccatg cacgcccacc
     7021 gccggcagct tgaggggatt gaacagggca tcgatcgcct cgatcgtgcg gccgctgacg
     7081 aatgcgatcg caccgccgag cctgtcggcc agctgctgca gcactggcac cagggtgtgc
     7141 ggcacctcca cggcctcggg gctgggggcc agctcgagca aggtaccgtc gatatcaaga
     7201 aacagggcgt gacccgtcag gatgtcccgt gccgcggtct ttttgaacag tgtctgcatc
     7261 cgttaaccgt agccaaccgg cgcgcggcgc gactatagga tcatgcgagc aaggctgtag
     7321 tccagaggga agttcgtata acgtagtcat atgaatcgga gcgcattgca agcacggatc
     7381 gaagccgctt acgagcgccg gcaggagctc agcgccggca cagtcgatgc ggagactcgt
     7441 gccgccatca tcgaggcggt cgccggcctc gatcgcggcc agttccgcgt cgccgaaaaa
     7501 atcgacggcc gctggcaggt ccacacctgg ctgaaacaag ccgtgctgct gtatttccgc
     7561 atccacgaca accagctcgt cgagggcgga ttcacgcgct tctacgacaa ggtgcctctc
     7621 aaatatgcgc agcacgatgc ggcgcgcttc gccgccgagg cagtacgcgt ggtgccgcac
     7681 gcgatcgtgc gccacggcgc ctatgtggct gcgaatgccg tgctgatgcc ctgcttcgtc
     7741 aacatcggcg cgtacgtggg tgccggcacc atggtcgata cctgggcgac cgttgcgtcg
     7801 tgcgcccaga tcggcagcaa cgttcacctg tccggaggcg tcggcatcgg tggtgttctg
     7861 gagccgctgc aggcaactcc gacgatcatc gaagacaact gcttcatcgg tgcgcgctcc
     7921 gagatcgtgg aaggcgtcat cgtcggcgcc ggatcggcga tctcgatggg cgtgttcatc
     7981 ggcgcgaaca ctccgattta tgatcgcacc actggcgaga cattccgcgg tgcggtccca
     8041 ccggggtcgg tcgtgatggc gggcagcatg ccgtcaaaag acgcaacaca cagcctgaac
     8101 tgcgcggtga tcgtcaaacg tgttgacgcg cagacgcgcg ccaagacctc gctgaatgag
     8161 ctattgcggc cgagtgactg agcgtcgcct agccgtagcg gccggtaatg taatcctcgg
     8221 tcgccttctt gccggggtta gtgaagatct tcgaggtctc gtcgaactcg atcagctcgc
     8281 cgaggtacat gaacgcggtg tagtcggaaa cgcgcgccgc ctgctgcata ttgtgcgtga
     8341 cgatcagcac cgtgacctgc tctcgcagcg ccgcgatcag ttcttcgatg cgggcggtcg
     8401 cgatcggatc cagagccgag gtcggctcgt cgaacagcag gatttccggg ctggtcgcga
     8461 gcgcacgcgc aatgcacagg cgctgctgct gaccgccgga tagtgacagg gcggaactgg
     8521 acaggcggtc cttgacctcg tcccacagcg ccgaaccgcg tagcgccttc tcaacttcct
     8581 cgcggatgcg gatctgtgaa cgcacgccac ggatacgcag tccgtacgca atattctcaa
     8641 agatcgactt cgggaacggg tttggcttct ggaacaccat gccgatgcgc atgcgcactt
     8701 cgatcggatc gacatcgcgg ccaaccagat ccatgttctc cgggaacaac gtgatgttgc
     8761 cctcgtaccg gcagcctggc gtcagatcgt gcatgcggtt gaagcagcga aggaacgtgc
     8821 tcttgccgca gcccgacgga ccgatcaggg cagtcacctg cttctcgcgg atctccatat
     8881 tgagatcctt gagcgctttg aatgcgccgt agaaaaaatt caggtgctcg gtcttggctt
     8941 tgatcggcgc cttgagcgcg gccgagcgac gtgcctcgtc cactgccacc tgtagcatgc
     9001 cccgaccgct cgtccggatc ggaataaccc ccgtgtcgat gtcgctcgca ggaagaaaac
     9061 cggacgactc tctctcggag ggcatgcctt tcaccaatgt tgccatgtgc gtcagtgact
     9121 tcaggctttg ggcgcgtgaa gctctttttc gatctcttct agtttcgtat gacgcacgtc
     9181 tttgccgtgc accaggtaga tcaggtattc gcagatattt ttggcgtgat cgcctatccg
     9241 ctccagcgcg cgcaccaccc acatcacatc cagcgcccga ccgatcgagc ggggatcttc
     9301 catcataaat gtgatgtttt ggcgatggat cgcctcgtac tcttcgtcca caatccggtc
     9361 gcgccgcagc acctgtagcg ccagatcggt gtcatagcgc gcaaacgcgt ccagggtgtc
     9421 gtgcaccatc gcgatgacca gctccccgag gttacgcagc tcgcggtagc ggttgttcgg
     9481 ccgctcacgc gccgccaggc gtgcggcaat cgccgccact ttctcgccct cgtcgccgat
     9541 gcgttccagg tcggtaatgg tcttcaggat cgagatgatg aggcgcaggt ccgatgccgc
     9601 cggtccgcgc gtcgccagaa tccggctgca atcctcgtcg atggagcgct cgagctggtt
     9661 gacccgcagt tcctcctgcg ccacccgcag catcaccttg gcatccggct cggtaatccc
     9721 gccgatcgcc tgaatcagtt gccgctcaac gaggccgccc atctcgagga cactgctgcg
     9781 caatcgctcc aggtcctcgt tgtagcggct ggaaatgtgg tggctgacgc cctcggtact
     9841 catccaccca tgttaatcgt ttgcccgtga cagatttgtg tcagcgatcg cctcaggagc
     9901 cgctcccaca tcctaaccgc ctgaatctgc ggcactcttg cgcccagccg tgacgaaatc
     9961 gcggtccggg caggctggat gctgtctgca gcgggtccgg ccaagacttt cctaaaggcg
    10021 cgggccggta ctggccccgg gcaggcttgg cggacgcgga cttggaaagg cctgcgcgct
    10081 ggatgttcga cggcttcgac atcgtcgaaa acttgcgcgt gatcactgtc gcgcgccggg
    10141 cgcacaagga gcccgagggc gatctgtcct ccgcaagcta ttgtcgacac actgcgggca
    10201 tcgtataagc gcccggagga acacgcatgt cagcaacgct cgaactggcc cacgaattga
    10261 tggcacgtgc atcggtgacg cccgcggatg agggctgtca ggagctgatg gccgcgcggc
    10321 tgagcaagct cggcttcaag gtggagcgcc tgcgctacgg cagcgtggat aacctgtggg
    10381 cccgccgcgg cggtaacggc ggcccggtgc tgtgcttcgc cggccatacc gacgttgtgc
    10441 cg
//
LOCUS       DATMRM010000508         5588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3712670, whole genome shotgun sequence.
ACCESSION   DATMRM010000508 DATMRM010000000
VERSION     DATMRM010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3712670"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VNZ06_15490"
     CDS             <1..305
                     /locus_tag="VNZ06_15490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22204.1"
                     /translation="LEQGYAWTYDRLFSHRSIWRRRPHDPRAVLAYLAMSYLYKRSNR
                     FWHLLIRHRLTAVVWRPLIELSRRRHLKFRDRLLHLPPDGPLLGAPQAGAVVSAGV"
     gene            307..864
                     /locus_tag="VNZ06_15495"
     CDS             307..864
                     /locus_tag="VNZ06_15495"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWW22205.1"
                     /translation="MTSYTGKRAFDLAVAGSACIVFGPVFAALAVVGVLEDGGPALFI
                     QRRVGSARRPFQVFKFRSMRDGEITRVGQWVRRTGLDELPQFLNVCRGEMSVVGPRPL
                     TPEDIQRLGWNDPSFDWRFAPKPGITGLSQLLAGQGVRASRRLDRLYLRRQSPLLDLQ
                     LVALSFAVNLIGKRRVRQMMRMGLR"
     gene            complement(872..1555)
                     /locus_tag="VNZ06_15500"
     CDS             complement(872..1555)
                     /locus_tag="VNZ06_15500"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HWW22206.1"
                     /translation="MLLEDAQHFVETALVAIAALLPIVDPLGGAPIYLVMTAGLSAAE
                     RARMAQLVAINSFVLLVASILVGAYVLDFFGVSIPAVQVAGGFIVCAIAWSLLTSPST
                     PAVSERSTAAAPEALSQRAFYPITMPLTVGPGSISVAMTLGANPPKGIRSLVTTALAH
                     VLGVLVVALSIYLCYRYADRILRKLGTTGTNVLIRLTAFILFCIGVQIMWNGLRTLLM
                     GLLRPTLGA"
     gene            complement(1568..1843)
                     /locus_tag="VNZ06_15505"
     CDS             complement(1568..1843)
                     /locus_tag="VNZ06_15505"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HWW22207.1"
                     /translation="MCRSIKTLFNFEPPATKEEVHAASVQFVRKLSGFHTPSKANQPA
                     FERAVRDICAAAEALMASLVTNAEPRDRAVEAAKAKARGAARFAARR"
     gene            2006..4423
                     /locus_tag="VNZ06_15510"
     CDS             2006..4423
                     /locus_tag="VNZ06_15510"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWW22208.1"
                     /translation="MSRNAPALLHILDRDTELDPEGRLSRRTLGRRLNLTAVALACSL
                     VALVCATFLLARHSQLVRATSAQQSRLIGWQQLLQSVDTYLTDANAAQRDYLLTGDAR
                     FLAPYQEAVHHMPEVLQKLDGLPISDPIVAAGIQDIRQQSARKLDELGRTLAVYGQSG
                     RDAAIAQARATMDQGGLQRVRDDTTSITNSIRANRRAGSQRVTAGINQIQQLAIVTVA
                     ALFLSVILAGFQIRRLIVTHILDRRSLEASEQRHRAIVEEQTEFIALSRRDGSIKYAN
                     PAYLRYFQVSASDLAAANLYHSIVPTDALVMRTRLTETLSGQHLSMETRVAGPDGTQR
                     WVWWRHRIQMIDGEMVIHSVGRDTTVAKQVGLALKASKEFLARTGRVAGVGGWELDLK
                     ERQVHWSDELRQMCEVTSDFVPTIENVIDFYKPEVRTQIRRAVDDCISAGTPWDFELP
                     LLTASGKEIYVRAVGEAEFDPDGKTIRLVGAMQDITDRKAMEVKELATRADLATQTAT
                     LNAVIESIPAMVAVWDTELRYRLVNKAFERWRGKRREDLIGHTMEEVVGNTEYPRSLE
                     WVHRALSGETVSYEKEYPGARESRHVSITYIPLRMKDGQVGGFVEVAQDITLHREEHV
                     RLTMLSERDPLTGLLNRAGLESYLRKKTEQGDGASLAMLYIDLDHFKAINDSYGHMTG
                     DDVLREFAARLQNLVRPTDAVARLGGDEFGIVLYGIHQPEHAVAVADKVVEFARQPFS
                     ISNLSLTIGASVGIAFDADSQGGWTGLVSRADALVYQAKASGRGRTAMGPADGMLPTD
                     RAVRPTG"
     gene            complement(4424..>5588)
                     /locus_tag="VNZ06_15515"
     CDS             complement(4424..>5588)
                     /locus_tag="VNZ06_15515"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HWW22209.1"
                     /translation="RYYGQPARFNYWNGCSTGGRQGYLLAQELGNELDGVMANAPAIY
                     WTRFQTAQMWGQMAMRQLVGSPIIPAKLQQVRESAIAACDAADGVTDGIIQDPRACHF
                     SAQANVCGTPTAPRSNCLTADEAAAVDKIWAGPRNAQRSMIWYGLDRGTDFATMDGPR
                     PFALGLTQLHWDMHDRNFDWQSVSLDEYAVVAEQGSRAIADLTDTTGPLDTFRRHGGK
                     LLTFVGTNDQSIFPRGVIGYYRQMAVRYGMRGSSDFSSIQDFYRLFLAPGVGHCGGGV
                     GPAPTDPFNALVNWVEHGVAPATLLAAGGSAAPPTGRTRPLCPYPSKAIYNGSGSIDD
                     AASFHCGDSPGEPSLVCQDVPVRYKHEKKGAPDYQAVGLSAGFCRTAHLTEQR"
BASE COUNT         1095 a         1710 c         1730 g         1053 t
ORIGIN      
        1 aactcgagca gggatacgcc tggacctacg atcggctgtt ctcgcaccgc tcgatctggc
       61 gccgacgacc gcatgatccg cgcgcggtcc tggcgtatct ggcgatgagc tatctgtaca
      121 agcgctccaa tcgcttctgg catctgctga tccgacatcg gctgacagca gtcgtttggc
      181 ggccgctgat cgaactatct cggcgacgcc acctgaagtt tcgcgatcgt ctgttgcatc
      241 tgccgccaga cgggccactg ctgggcgcac cgcaggcggg cgcggtcgtc agcgctggag
      301 tctgatgtga cttcctacac tggaaaacgc gctttcgact tagctgtggc aggcagcgcc
      361 tgcatcgtct tcggtcctgt gttcgctgcg ctcgcggtgg tcggcgttct tgaagacggc
      421 ggaccggcgc tttttatcca gcgacgagtc ggcagcgcac gtcggccgtt tcaagtattc
      481 aaattccgaa gcatgcgcga tggtgagatc acgcgcgtcg gccagtgggt ccgccgcacc
      541 gggcttgatg aactaccgca attcctgaac gtctgccgag gagagatgag tgtcgtcggt
      601 cctcggccgc tcacccccga ggatatacag cgcttgggat ggaacgatcc gagtttcgac
      661 tggcgcttcg cgcctaagcc gggcatcacc ggcttgtcac aactgctggc gggtcaaggg
      721 gtgcgtgcca gccggcgcct cgaccggtta tatctgcgac ggcagagtcc actgctcgac
      781 ctgcagctcg tcgcgctatc gtttgcggtc aacctgatcg gcaaacggcg agtgcgacag
      841 atgatgagga tgggattacg atgacgaccc cctaggcacc aagcgtcgga cgcagcaagc
      901 ccatgagcaa cgtgcgcagc ccgttccaca tgatttgcac accgatgcag aaaaggatga
      961 acgctgtcag acgtatcagt acattggtgc ccgtggtacc cagttttcgc aggatccgat
     1021 ctgcatagcg gtagcagaga tagatcgata acgcgacgac caaaacgccc aggacatgcg
     1081 ccagcgcggt ggtcaccaag gagcggatcc ccttgggcgg gttggcaccc agagtcatcg
     1141 cgaccgagat cgatccgggc cccaccgtca acggcatggt gatcggatag aacgcgcgct
     1201 gactgagcgc ttcgggagcc gctgccgtgg agcgctccga gaccgctggc gtgctggggc
     1261 tggtcaatag cgaccaggca atggcgcaga caataaatcc gcctgccact tggaccgccg
     1321 gtatggatac cccaaagaag tccagcacgt aggccccaac caggatcgac gccaccagca
     1381 acacgaagct gttgatcgcc acgagctgag ccatccgggc gcgctctgcc gcagacagtc
     1441 ccgcggtcat caccagataa atcggggcgc cgccgagcgg gtctacgatc ggcaacagtg
     1501 ctgcgatcgc gaccagcgcg gtctcaacaa aatgttgggc gtcttcgagc aacatcggcg
     1561 cttgcactca acgccgcgcg gcgaacctcg cagcgccccg cgccttggcc ttggcggctt
     1621 cgaccgctct atcgcgtggc tccgcgttcg tgaccagcga cgccatgagc gcctcggcgg
     1681 cggcgcagat gtcgcggacg gcgcgctcga aggccggttg attggcctta gacggggtgt
     1741 ggaaaccgct cagtttgcgg acgaattgca cggaggcagc atgaacttcc tccttggtag
     1801 ccggcggttc gaaattgaac agggttttga tgcttcggca catattttcc acctgggtgc
     1861 ggactgacgg atgcgacttt actacgcatg ctccccgaag gcttaccgcg ggcaaaacgt
     1921 gaactgagtc tcatttgcgc cctgtccgat gcgtatctcg tctcccattt aagggaaacg
     1981 agagttgatt cctgagcaga ttctcgtgtc gcgcaatgct cccgctttgc tgcatattct
     2041 ggaccgagac accgagttgg atcccgaagg acgactctcc cgtcgcacgc tgggtcggcg
     2101 gctcaatctc acggcagttg cactggcgtg ctctctggtc gcacttgttt gcgcgacctt
     2161 cctactggca cgccactccc aactggtgcg cgcaaccagc gcccagcagt ccaggctcat
     2221 cggatggcaa caactgctgc aatcggtgga tacctatctg accgacgcca atgctgcgca
     2281 gcgcgactat ctgttgaccg gggatgcgcg gtttctggcg ccgtatcagg aagcggttca
     2341 ccacatgcca gaggtcctgc agaagctgga cggtctgccg atctctgacc ccattgttgc
     2401 ggcgggcatt caagatatcc gtcagcagag cgctcggaag ctggatgagt tagggcgaac
     2461 gctcgcggtg tacgggcaga gcggacgaga cgcagccatc gcgcaagcca gggccacgat
     2521 ggatcaaggc ggactgcaac gcgttcgcga cgacacgacg tccatcacga atagtattcg
     2581 cgccaaccgc cgcgccggca gccagcgagt cacggccggc atcaaccaga tccagcagct
     2641 cgccatcgtc acggttgcgg cgctgttcct gtcggtgatc ctggcgggct ttcagatccg
     2701 acggctgatc gtcacgcaca tcctggatag gcgcagtctg gaggcgagcg aacaacgcca
     2761 ccgggcaatc gtcgaggagc agacagagtt cattgcgcta tcacggcgcg atggctcgat
     2821 caagtacgcc aatcccgctt acctgcggta ttttcaggtg tcagcatccg atttggccgc
     2881 tgccaatctc taccattcaa tcgtcccgac cgatgcgctg gtgatgcgca ctcggttgac
     2941 cgaaacgctg tccggccagc atctctcgat ggaaacccgc gtcgccggac ccgacggcac
     3001 gcagcgatgg gtctggtggc gtcatcgcat tcagatgatt gatggagaga tggtcattca
     3061 ctcggtcgga cgtgacacca ccgtcgcgaa acaggttggc ttagctctca aggccagcaa
     3121 ggaatttctt gcgaggaccg gccgggtcgc gggtgtcggc ggatgggagc tcgatctgaa
     3181 ggaacgccag gtgcattggt ccgatgaact gcggcagatg tgcgaagtga ccagcgactt
     3241 cgtgccgacg atcgagaatg tcattgattt ctacaagccc gaagtacgta cccagatccg
     3301 ccgggccgtg gatgactgca tcagcgcggg cacaccctgg gacttcgaac tgccgctgct
     3361 gaccgcttcg ggcaaagaga tctacgtgcg cgcagtgggt gaagcggagt tcgaccctga
     3421 tggcaagacc atccggctgg tcggtgcaat gcaggacatt accgaccgca aggcgatgga
     3481 ggtgaaggag cttgctactc gggcggattt ggcaacccag actgccaccc tgaacgcagt
     3541 gatcgaatcc attccggcca tggtagccgt gtgggatacc gagttgcgct accggctcgt
     3601 gaacaaagcg tttgaacgct ggcgcggcaa gcgccgcgaa gacctcatcg gccacacgat
     3661 ggaagaggtc gtcggcaata cggagtaccc gcgaagtctc gagtgggtcc atcgggcgct
     3721 gtccggcgag accgtctcct acgaaaagga atatcccggc gcgcgcgagt cacggcatgt
     3781 gtcgatcacc tacataccgt tgcgcatgaa ggatgggcag gttggaggct tcgtcgaagt
     3841 ggcgcaggac attaccctgc accgtgaaga acatgtacgc ctgacgatgc tgagcgaacg
     3901 cgacccgctc acggggctac tcaatcgcgc cgggctggaa agctatcttc gcaaaaagac
     3961 cgaacagggc gatggcgcct cattggcgat gctgtacatc gatcttgacc acttcaaagc
     4021 gatcaacgat tcctacggcc acatgaccgg cgacgatgtg ttacgtgagt ttgccgcgcg
     4081 gctgcaaaac ctggtgcgcc ccacggatgc ggtggctcgt ctcggcggcg atgagtttgg
     4141 aatcgtcctc tacggtatcc atcagccgga acacgcggtc gcagtcgccg acaaggtcgt
     4201 cgagttcgcg cgtcagcctt tcagcatctc taacctgtcc ctgacgattg gtgcgagtgt
     4261 cggcatcgcc ttcgatgcag acagccaggg cggctggacc ggtctggtct cgcgcgctga
     4321 cgcgctggtc tatcaggcca aggccagtgg tcgcggtcgg accgcaatgg gtcctgccga
     4381 tggcatgctg cccactgatc gcgcggtgcg accgacgggc tgatcatcgt tgctctgtca
     4441 ggtgcgccgt gcggcagaac ccggcgctca accctacagc ctggtagtca ggtgcgccct
     4501 tcttctcatg tttgtagcgg accggaacgt cctgacaaac gagggatggc tcccccggac
     4561 tatcgccaca atgaaagctg gccgcatcgt cgatactgcc gctgccgttg tagatcgcct
     4621 tgctgggata cggacataga ggacgggtac gacccgtggg cggcgctgcg cttccacccg
     4681 cggccagcaa ggtggcggga gcgacgccgt gctcgaccca gttcaccaat gcgttgaagg
     4741 gatccgtcgg cgccgggccc acacccccgc cgcagtgccc aacgccgggt gccagaaaca
     4801 agcggtagaa gtcctgaatc gagctgaaat cggacgagcc gcgcatgccg tagcgaacag
     4861 ccatctgtcg gtaatatcca atcacacctc gcggaaagat ggactggtca ttggtaccca
     4921 cgaaggtcaa cagcttgccc ccgtggcggc ggaacgtatc cagtggcccc gtggtatcgg
     4981 tcaaatccgc gattgcgcgc gagccctgct cggcgacgac cgcatactca tcgagcgaca
     5041 cggattgcca atcgaaatta cggtcgtgca tatcccagtg caattgcgtc agtccgaggg
     5101 caaacggtcg cggaccgtcc atggtggcga agtcggttcc acggtccagt ccataccaga
     5161 tcatggaccg ctgcgcgttg cgaggacccg cccagatctt gtcgacggcg gccgcttcgt
     5221 ccgccgtcaa gcagttgctc cgcggagccg ttggcgtacc gcagacgttg gcctgggcac
     5281 tgaaatgaca ggcgcgcgga tcctgaatga tcccgtcagt cacgccgtcg gcagcatcac
     5341 aggctgcgat tgcggactca cgtacctgct gcaacttcgc aggtatgatc ggtgaaccca
     5401 ccagttggcg catcgccatc tgtccccaca tctgagcggt ctggaaccga gtccagtaaa
     5461 tggccggcgc attcgccatg acaccgtcga gttcgttgcc cagttcctgt gccagcagat
     5521 agccctgtcg gccaccggtg gagcatccgt tccagtagtt gaatctcgcg ggttgtccgt
     5581 agtatcgc
//
LOCUS       DATMRM010000509        17012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3395851, whole genome shotgun sequence.
ACCESSION   DATMRM010000509 DATMRM010000000
VERSION     DATMRM010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17012
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3395851"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..2006)
                     /locus_tag="VNZ06_15520"
     CDS             complement(141..2006)
                     /locus_tag="VNZ06_15520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HWW22210.1"
                     /translation="MTRKWLLPLVLTLSAGSGMASLAQDSTPPEMWDLGDLYPNTAAW
                     DQTLARTRAAADKIASYQGTLSKSADVMFSALDAMSAANREAARLFVYASLKSDEDVR
                     IGANIERKQLAQALVTQLGERSAWVAPEIVKIGAAKIKTFTDANKQLKGRFGFFLDNT
                     LRAAPHTLSPESEGVLAMTGDLLSQPGTLHNQFENGDFPAPTVTFSDGSKVRLDEQAY
                     EKYRQVSNRGDRKLAFDQYWSTWKRFEGTAGGLLTTQVMGNHFTAEARRFPNALQAAQ
                     FPDNMPEGVYRSLVAEVNAALPALHRYLRLRKRQLGIAGEMHYYDVYPAFALRKPPSF
                     TVAESERITLDALKPLGEDYLSLMRKGFSSHWMNAYPHEGKASGAYMNGAAYDVHPYL
                     LLNHQNDYASLSTLAHEWGHAVHTLLTNEAQPFEKSNYSTFIAESASIGNEMLLNDYM
                     VEHAANRSEKLYYLGQGVDSIRGTYFRQVMFAEFELAIHEELEKGKALSGERMTELYC
                     GILKKYYGEAEGVMKIDPGYCVEWAFVPHFYLDFYVYQYATSMVGAASFTDAILKEGA
                     PARGRFIRMLQAGGSEYPYDLYKQAGIDMATPEPYRALAARMNRLLDEIEALEKQ"
     gene            complement(2016..3656)
                     /locus_tag="VNZ06_15525"
     CDS             complement(2016..3656)
                     /locus_tag="VNZ06_15525"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22211.1"
                     /translation="MQITRVVRWLILLLAPMAAVQAADNGDVDKKDQDKKEAPKPPDH
                     FTAEQVDTAGSVTIEGRRIDYEAHTGTLVVHAHDWDDVPQNAPKDEDKKPRPEASMFY
                     VAYFKSDKSEAKSGSARPEPRPITFLYNGGPGSATVWLHMGAFGPKRVVTANDSHTPA
                     APYSLVDNESSLLDVSDLVFIDAPGAGFSRIAGTDRDKAFYGVDEDAWAFSDFVVQFL
                     GKYGRWNSPKYLFGESYGTTRNAVLVNLLETGRSVDFNGVIMLSQILNFDGSPDGPDT
                     NPGIDLPYQLALPTYAATAWYHHKLANAPSDLPTFLKDVERFAMSDYALALEAGWTLS
                     DDRRRAVAQKLHDYTALPVDYILKANLRIDGGEFEHSLLSDTDMTTGRLDTRFAGPSL
                     DPLSKEADYDPQSAAISSAYVSTFNDYVRKQLKYGQDKIFKPFANIDHWDFSHRGPGA
                     QEALQQSTNVMPDLATAMKVNPGLHVLLTGGYFDLATPYYQGVFEMQHLPIPSAVQKN
                     IEFAYFESGHMVYANENSLKQMHAKVADFIHRTQGPKS"
     gene            complement(3698..5926)
                     /locus_tag="VNZ06_15530"
     CDS             complement(3698..5926)
                     /locus_tag="VNZ06_15530"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWW22212.1"
                     /translation="MYRTIIFRTVLPLLLAAGVVAYLGLPYIDRLLTNWFQSDVELRA
                     RLVISSIEETLPALLATHDQPGLRKYASKITADQRLVGLLICGADGWTEFKTERVPGA
                     VSCDSTAKLADGQSDVMRASWGSVLVSAFDEKPQGQAPYRVEIVNDLSFVDRRQDKAR
                     DFLITLACITLVIVALFLVLAAWLVIRRYANMLLRDISGRRFLDNAQSGSLSLPFLKQ
                     VHQVLREMEESQRLEIDYRENWTPQALQQVVREQLHSPQMLVVSNREPYSHEYGEHDS
                     IQVQVPASGMVTALEPVMRACSGTWVAHGSGTADREVVDRNDRVKVPPEDPSYTLRRV
                     WLSPPEEEGFYYGLSNEGLWPLCHLAYVRPAFRGEDWVAYQAVNRKFADVVLREAQSD
                     DPVVLIQDYHFALLPGLLRARRSKSTIAMFWHIPWPNAETFGVCPWKHEMLSNLLMSD
                     ILGFHTLYHCQNFLATVDRFIECQIDHAHMTVTLQGHVCHVAAYPISIEWPPRWLVAV
                     PDVTLSRRRVREHFHISEHVTLGLGVERWDFTKGVLERFLALELLLEQDPGMRGNVTL
                     LQIAAPSRSKLPAYQALQQATLSEVERINERFATADWKPIVLITRHQPPEQVFELYRA
                     ADFCIVNSLHDGMNLVAKEFVAAREDEDGVLILSTFAGASRELVEALLVNPFDVAETA
                     AAIRRAVDMPREQRRQRMQLMRRTVKENNVYRWAGRMLMDAARIRQRQALLTGEEELR
                     LT"
     gene            6039..7157
                     /locus_tag="VNZ06_15535"
     CDS             6039..7157
                     /locus_tag="VNZ06_15535"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HWW22213.1"
                     /translation="MTTLQIVCLALAVSALTLALIFGVFRSGKPPAVAAAPPSTPAQS
                     ARPAAAVAPAVPATQDEPPEELNARFEATHRLQLLALSSEAAPPADSPVHEEIASKVR
                     WTLATITDKPNYAPRRPLLLPRLVQAMNNDDVSRRELARIIASDPGLAAGLFRLANSP
                     FYRVSPTPIESLDRAVALLGIEGMRSLVAAALMQPVFKVDGGGFPRFGDIVWQHTLAS
                     ASAAESHAVMLETTDPFAAQLLALMMGLSTIVVFRVAIDEYQGRHLAPHPRVLRALID
                     SQSAPVARQIAASWQLSERVNAALVDQTAPQGSKLTSLGRSLQFGRFLGALAVLHQQQ
                     VLDDEAVSAALRTGGSLAPAYERIWAKRAALQNEAHRP"
     gene            complement(7161..8180)
                     /locus_tag="VNZ06_15540"
     CDS             complement(7161..8180)
                     /locus_tag="VNZ06_15540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWW22214.1"
                     /translation="MIDRMIPFSVLDLSPIVEGATAAEALRNTLALARHVEPLGFKRF
                     WLAEHHNMPGIASAATAIVIGHVAAGTRSIRVGSGGIMLPNHSPLVIAEQFGTLASLY
                     GDRIDLGLGRAPGTDPETARALRRDLVSSGEEFPRDVQELQHYFEGSSAQTLRAVPGA
                     GLKVPIWLLGSSMFGAQLAAMLGLPFSFAAHFAPDLLLPALSIYRERFESSNQLSKPY
                     AMPCINVIVADTDEEARWLLSSHQQAFMNLRRGRPGKLPAPRDDMNAFWSPGERTMVE
                     HAMRYTFAGSAASVEHSLRGFLRLTQADELMVNVHVYDQAARQHSLTLTAAVRDRIAN
                     APSLL"
     gene            complement(8190..10853)
                     /locus_tag="VNZ06_15545"
     CDS             complement(8190..10853)
                     /locus_tag="VNZ06_15545"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HWW22215.1"
                     /translation="MNRRVAVIGAVALCPIACTLRDHPPHQPESARVERLLTQMTLEE
                     KISLLHGVPEDPATDQGEAGYLPGVPRLGIPPLRFADGPPGVLTRYPATALTATMGLA
                     ATFSLEDARANGAVIGRDARNLGVDVALQPFIDLHRDQTFARAYNTFGEDPLLTGMIG
                     AAMIRGIQGEGVMAQAKHFIGYDGANDVTMNAQALHEIYLAPFDAAVRARVSSIMCSY
                     NVINGAYACGNRALLTGVLRDELKFGGFVTSDWGALHGTSFIEAGDDLEMPGSGTTMD
                     SYFEAQLPRPGSLRKPLRGPEISPIPEEYAPDTPAFVLTVPMDRPIGMLSALERGTVS
                     EQTITRAAGRILMQMQRFGLLDRKGAAPVRGVQVGRAELANAETVLKTSEDAAVLLKN
                     SVNALPLIAADLHSLALIGPGALQDIAAGEAGEKALGRVERQTSPAEAIARLAGARPD
                     AGARIERAVADDMNGRTVPVSFERLDTQGQVIGQDPSLEFTRARGNALAPGSSFSWSG
                     TLPIPVNGRYRLYLQVLGASATMEVDGRRVAHSGALDLHGNLVQPGQDNVLPSLDGLD
                     DVRREMTLTAGAHPVTVHVTGERAGQPVQVRLAWVTPEQRNADYQQAIEAAAHTSKAI
                     VFAWSRGRPAFGLPGDQDQLIADVAAVNPNTIVVLNLSEPVAMPWLNKVKAVLLMWWP
                     GDEGGPATANLLLGRTSPGGRLPFTWPQRLDQNVANDPAHPERSSFGVNGRTQYSEGI
                     FIGYRWFDQQGLEPLFPFGFGLSYSRFEYSDLWAHAAADGGLDISVQLRNTGAYASDE
                     VVQVYLGAPERAVSDPGTQFAPRALAAFQRVHLAAGESRRLTLHAQVRALQYWSAARA
                     RWVTAVGARTVYVGAASRDLRLHAVTIIPPP"
     gene            complement(10919..11434)
                     /locus_tag="VNZ06_15550"
     CDS             complement(10919..11434)
                     /locus_tag="VNZ06_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22216.1"
                     /translation="MTGAGAGAARSSTTPTNGTQLASAFENLISQINSANPAGNTRGV
                     AATANPGLQAVNGLHHRHGGLALSALLQQLDELAAGTTGPSSAASASTAGATAVNTAG
                     TTTSAGAASASNVEATLNQLLQNLKLAASSTNVAALNSATAAHSAQSLAQRLAQQGVQ
                     ARAGHLLSAVV"
     gene            11759..12436
                     /gene="trmB"
                     /locus_tag="VNZ06_15555"
     CDS             11759..12436
                     /gene="trmB"
                     /locus_tag="VNZ06_15555"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HWW22217.1"
                     /translation="MQRGIRSYVIRTGRITDAQRRAREQHWPYFGIEFQPQLLDLEHC
                     FGRRAARTLEIGFGNGEHLLERAQCSPERDFLGVEVHEPGIGHLLLAAARAQLPNVRV
                     IAHDAVEVLQHQIAPDTLDEVQLLFPDPWPKKRHHKRRIVQPDFALLVASRLSPEGRF
                     HLATDWTPYAEQMLNVLNDCPLLVNCATDLGFVGPEEVMTRQATRFQRRGERLGHSVH
                     ELLYRRR"
     gene            12652..12978
                     /locus_tag="VNZ06_15560"
     CDS             12652..12978
                     /locus_tag="VNZ06_15560"
                     /inference="COORDINATES: protein motif:HMM:NF042799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6763 family protein"
                     /protein_id="HWW22218.1"
                     /translation="MSAQPDIGEWYRIRGGDQFEVVAIDEDDGTIEVQYFDGTVEEID
                     IGDWEAQHANGEIAEAETQDDWSGSVDGDDDNEEFALTSHFDSRPHTSGGLDGLDLFE
                     SPDYFQ"
     gene            complement(13000..13386)
                     /locus_tag="VNZ06_15565"
     CDS             complement(13000..13386)
                     /locus_tag="VNZ06_15565"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWW22219.1"
                     /translation="MTATEIDLARVLCRIADLEGGCREFRLGRGDWPLRGFVVQVGDG
                     VRAYVNRCAHLSYPLNYLRHQFLTHDGSMIQCYVHGAIFEKSTGYCVAGPCSGLSLIA
                     LPVRVEAGYVLLADEANPDELAQRYA"
     gene            complement(13383..14582)
                     /locus_tag="VNZ06_15570"
     CDS             complement(13383..14582)
                     /locus_tag="VNZ06_15570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWW22220.1"
                     /translation="MLAIMLLGAASGFPNQLTESALQAWLKDSAVSLTKIGVMSYVAL
                     PYLLKFLWAPLIDRYPLPLLGRRRGWILVMQILLAAAIALFALQDPAQTLVPIAVCAV
                     AIVFFSATQDIGIDAWRTDVSTPSERGPAAAVTNLGYRSAAWLASAFALLVAEHFGWR
                     PAFLILAGIMILFCFSTWFAPEPASARASPSSLRESVITPLRELLAGPGIKALLVVVL
                     LFKIGDAFANKLFTPFMLDVGFSKQEVALVVKPLLTAGAIGGAILGGIMMVRLGLLRS
                     MLIFGASQALTNLLYCALAATGKNFPLMVGAVLTEHVAGAMGNIALIALIMALCDLRY
                     SAFQYALLSSIALLPRYALGYPAGWIADHAGWYAYFVVSFLIALPGLAIVWFTRHKIE
                     TLDRQHT"
     gene            complement(14648..15412)
                     /locus_tag="VNZ06_15575"
     CDS             complement(14648..15412)
                     /locus_tag="VNZ06_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWW22221.1"
                     /translation="MRIITLNVNGIRSAGKRGLFDWLGRQHADVVCLQETRALEHQWE
                     DTHQVPGYLKHLYAAERPGYAGVAVLSRREPDRVVRGFKIDEFDREGRYLELQFGKLC
                     VVSLYLPSGSAGPERQASKFRFLEAFMPHLRKLRRSRREYILCGDWNIAHKQIDLKNW
                     RSNQKNSGFLPEERAWMDQLLGSERYIDAFRSLVPEPDHYTWWSNRGAAYAKNVGWRI
                     DYQILSPGLAGTPRKAHIHKEPRFSDHAPLIMDYRL"
     gene            15436..16134
                     /gene="pyrE"
                     /locus_tag="VNZ06_15580"
     CDS             15436..16134
                     /gene="pyrE"
                     /locus_tag="VNZ06_15580"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HWW22222.1"
                     /translation="MLDYQRQFLDMALARQALRFGQFTLKSGRVSPYFFNAGLFNDGQ
                     SLATLGRCYAGALLHSQLQFDLLFGPAYKGIPLVASTAVAIADAQRRNVPWAFNRKEA
                     KDHGEAGLIVGSPLQGRVVILDDVITAGTAIRESIELIRQAGAEAVGVALALDRQERG
                     QTERSAVQEVESLYGVKCVTILTFSELITALEERAATVHGSAAGYPAPAPTIEQLAAM
                     RTYRLEYGVTSQTP"
     gene            16192..16818
                     /locus_tag="VNZ06_15585"
     CDS             16192..16818
                     /locus_tag="VNZ06_15585"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HWW22223.1"
                     /translation="MHRGWSILLSSLALGALGVAYASQSGPVYKWTDEKGIVHYGDSV
                     PPEYAQGERSVLNSHGVEVGHVEGTNNATAMAERARTAEAAKLRAQHDQFLLSTYLST
                     KDIEQLRDERLGQIDGQIKASNIYIDGLGTRMLALEDRATHFKPYSSDPKARRMPDDL
                     AEELVRVSNETRSQHQALDAKHRQQSDMRAQFDADIARFRELTAKPKS"
     gene            complement(16831..>17012)
                     /locus_tag="VNZ06_15590"
     CDS             complement(16831..>17012)
                     /locus_tag="VNZ06_15590"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298007.1"
                     /note="catalyzes the formation of dUMP from dUTP; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HWW22224.1"
                     /translation="QGPLMVSCWNRGSDSYRIQPGERIAQLVVVPVVQVALDIVQDFS
                     ASARGAGGFGHSGRQ"
BASE COUNT         3318 a         5401 c         5122 g         3171 t
ORIGIN      
        1 gttagcagct tgggaagctg cagttctacc attgaactac acccgcagta tctgcaactc
       61 tacagcgtcg aacacactga tcgcaacgaa gccctgaaag tcaatagctg agccaatgtc
      121 aggggagcct gcgtgaggcg ctactgcttt tcgagcgctt cgatctcgtc gagcaatcgg
      181 ttcatccgcg ccgccagcgc ccgatagggc tccggcgtcg ccatgtctat acccgcctgt
      241 ttgtagagat cgtacggata ctcggacccg cccgcctgca gcatcctgat aaagcggccg
      301 cgcgcaggcg caccctcctt gaggatcgca tcagtgaatg acgccgcgcc caccatggag
      361 gttgcgtatt gataaacata gaagtcgaga tagaaatgcg gcacgaacgc ccattcgaca
      421 caatagcccg ggtcgatctt catgacgccc tcggcttcgc cgtaatactt cttgagaatg
      481 ccgcagtaca gctcggtcat ccgctcgcct gacagtgcct tgcccttctc cagttcctca
      541 tggattgcga gctcaaactc cgcaaacatc acctggcgga aatacgtgcc tcggatcgaa
      601 tccacaccct gaccaagata gtagagtttc tccgagcggt tggctgcgtg ttcgaccata
      661 tagtcattga gcagcatctc gttgccgatg gaggcggatt ccgcaataaa ggtcgagtag
      721 tttgacttct cgaacggctg cgcctcgttc gtcagcagag tgtgtacggc atgtccccac
      781 tcgtgcgcca gcgtcgaaag cgaagcgtag tcgttctgat ggttgagcag caggtacgga
      841 tgcacgtcat aggccgcgcc gttcatgtac gcaccggatg ccttgccctc gtgagggtaa
      901 gcattcatcc agtgtgagct gaatcccttg cgcatcagcg aaaggtagtc ctctccaagt
      961 ggcttcagcg cgtcgagcgt gatgcgctcg gactcggcaa cggtgaagct cggcggcttg
     1021 cgcagcgcaa acgccggata gacatcgtag tagtgcatct caccggcgat gccgagctgg
     1081 cgcttgcgca gccgcagata gcgatgcagc gccggcaacg cggcgttcac ttcggcgacc
     1141 agcgagcggt agaccccctc gggcatgttg tcggggaact gcgccgcctg caacgcgttc
     1201 gggaacctgc gcgcctcagc agtgaaatga ttgcccatca cctgagtcgt cagcagcccg
     1261 cccgcagtgc cttcaaacct cttccaggta ctccagtatt ggtcgaacgc cagcttgcgg
     1321 tcaccgcgat tggaaacctg acggtacttt tcgtacgctt gttcgtccag tcggactttg
     1381 ctgccatccg agaaggtcac cgtgggggcc ggaaaatccc cattctcgaa ttgattgtgc
     1441 agcgtacccg gttgcgacag caggtcaccg gtcatcgcca gcacgccttc cgactcaggg
     1501 ctgagagtat gcggggcggc gcgcagcgta ttgtccagaa agaaaccgaa tcgcccctta
     1561 agctgcttgt tcgcatcggt aaaggtcttt atcttggcgg ccccgatttt cacgatctcg
     1621 ggcgccaccc acgcgctcct ttcaccgagc tgagtcacca acgcctgtgc caattgcttg
     1681 cgctcgatgt tggcgccaat gcgcacgtct tcgtccgatt tcaacgatgc atagacgaag
     1741 aggcgcgccg cctcacgatt tgccgcgctc atcgcatcga gtgcgctgaa catgacgtcg
     1801 gcgctcttac tgagcgtgcc ctggtagctg gcgattttgt ccgctgcggc gcgtgtgcgc
     1861 gccagggtct ggtcccaggc cgcggtattt ggatacaggt cgccgaggtc ccacatctcc
     1921 ggcggtgtcg aatcctgtgc cagcgacgcc atcccggagc ccgcactcag cgtgagcacc
     1981 agcggcagca gccactttct ggtcatcgcg atcccctaag acttcggtcc ctgagttcga
     2041 tggatgaagt cggcgacttt ggcgtgcatc tgcttcagcg aattctcgtt ggcgtacacc
     2101 atatgacccg actcgaagta cgcgaattcg atgttctttt gtaccgcgga cggtatcggc
     2161 agatgctgca tctcgaacac accctgatag tagggtgtcg ccagatcgaa atagccaccc
     2221 gtcagcagta catgcagacc cggattgacc ttcatggcgg tcgccaggtc cggcatcaca
     2281 ttggtggact gttgcagggc ttcctgtgcg ccgggtccgc gatgactgaa gtcccagtga
     2341 tcgatgttgg caaacggctt gaagatcttg tcctgaccgt atttgagctg cttgcgcacg
     2401 tagtcgttga acgtcgacac gtaggcagaa gaaatggcgg ccgactgggg atcgtaatcc
     2461 gcttccttgc tgagcggatc gagcgatggc cccgcaaagc gggtatcgag tcggcccgta
     2521 gtcatatccg tgtcgctgag cagactgtgc tcgaactcac caccgtcgat gcgcaggttg
     2581 gctttgagaa tgtagtccac tggcagcgcg gtatagtcgt gcaacttctg ggcgacggcg
     2641 cggcgccggt cgtccgatag cgtccagccc gcctcgagtg ccagcgcgta gtcactcatc
     2701 gcgaagcgct cgacatcctt aagaaatgtc ggcagatccg aaggcgcgtt ggccagcttg
     2761 tgatgatacc aagccgtcgc tgcgtaggtc ggcagcgcca gctggtacgg cagatcaatt
     2821 cctggattgg tgtccgggcc gtccggactg ccgtcaaaat tcaggatctg cgacagcatg
     2881 atgacgccat tgaaatccac cgagcgcccg gtctccagca gattcaccag caccgcattg
     2941 cgcgtggtgc catagctctc accgaacaga tacttgggcg agttccagcg tccgtactta
     3001 ccgagaaact gcacgacgaa atctgaaaac gcccatgcgt cctcatccac gccgtagaac
     3061 gccttgtcgc gatcggtgcc ggcgatgcgc gaaaagccgg cccccggggc atcgatgaat
     3121 accaggtcgc tcacgtcgag cagactggac tcattgtcca cgagcgaata gggtgctgcc
     3181 ggggtgtgcg agtcgttggc ggtgaccaca cgttttggtc cgaatgcgcc catatgcagc
     3241 cagaccgtgg ccgatccggg acccccgttg tacaggaagg tgatcggacg cggctccggc
     3301 ctggcgctac ccgacttagc ctccgattta tccgacttga agtaagcaac gtagaacatg
     3361 ctcgcttcgg ggcgcggctt cttatcctcg tccttcggcg cattctgggg cacgtcgtcc
     3421 caatcgtgcg catgtaccac cagggtgccg gtgtgcgcct cgtagtcgat gcgtcggcct
     3481 tcaatggtca ccgatccagc cgtatccacc tgctcagcgg tgaagtgatc gggtggtttg
     3541 ggcgcttctt tcttgtcctg atccttcttg tccacatcgc cgttgtctgc ggcctgcacg
     3601 gccgccatcg gcgcgagcaa cagaatgagc cagcgcacaa cgcgagtgat ctgcaccgga
     3661 gtccccttac ttcttttcga gagtgcagat cttacactta agtcagccgt agctcctctt
     3721 cgccggtcag cagcgcttga cgctggcgaa tgcgcgccgc gtccatcagc atgcgaccgg
     3781 cccagcggta gacgttgttt tcttttacag tgcggcgcat gagctgcatg cgctgtcggc
     3841 gctgctcacg cggcatgtcc accgcccgtc ggatcgccgc cgcggtctca gcgacatcga
     3901 acggattgac caacagcgcc tcaaccagtt cgcgcgaggc gccggcgaag gtactcagga
     3961 tcaacacccc gtcctcatct tcgcgcgcag cgacgaactc cttagccacc agattcatgc
     4021 catcgtgcaa actgttgacg atgcagaagt ccgcggcccg gtagagctcg aacacttgct
     4081 ccgggggctg atgcctggtg atcagcacga tcggcttcca gtccgcggtc gcaaaacgct
     4141 cattgatgcg ttcgacttcc gatagggtcg cctgctgcaa tgcctggtaa gcgggcaact
     4201 tgctgcgcga aggcgcagca atctgcagca gcgtgacatt gccgcgcatg cctggatcct
     4261 gttccagcag cagctccagt gccagaaacc gctccagcac tcccttggtg aagtcccagc
     4321 gttccacgcc caggccgagg gtcacatgct cgctgatatg gaagtgttca cgcacccgcc
     4381 ggcgtgacag ggtcacgtcc ggtacggcca cgagccagcg cggcggccac tctatcgaga
     4441 tcggataggc ggccacatgg cacacgtgtc cctgcagggt cacagtcata tgtgcgtgat
     4501 cgatctggca ttcgatgaaa cgatccaccg tcgccagaaa gttctggcaa tgatagagcg
     4561 tgtggaagcc aaggatgtcc gacatcagta ggttcgacag catttcgtgc ttccacgggc
     4621 aaacaccgaa ggtctccgcg ttcggccatg ggatgtgcca gaacatggcg atcgtggatt
     4681 tcgagcgtcg agctcgcagt agcccgggca gcagcgcgaa gtggtagtcc tgaatcagaa
     4741 ccaccggatc gtccgactgc gcctcgcgca acaccacgtc ggcgaatttg cggttcaccg
     4801 cctgataggc gacccagtcc tctccgcgaa atgcgggcct gacatacgcc aggtggcaca
     4861 gcggccatag tccctcgttc gacaatccgt agtagaagcc ctcctcttcg ggcggcgaca
     4921 accacacccg ccgcaaggta tacgacggat cctctggcgg caccttcacg cggtcgttcc
     4981 gatccacgac ttcgcgatcc gcggtcccgc tgccgtgcgc aacccaggtg cctgagcacg
     5041 cacgcatgac cggctcgagt gcggtcacca tgccgctggc gggcacctgc acctggatcg
     5101 aatcatgctc gccgtattca tgtgagtaag gttcacgatt ggagaccacc agcatctgcg
     5161 gcgaatgcag ctgctcgcga accacctgtt gcagtgcctg cggcgtccag ttctcgcgat
     5221 agtcgatctc caggcgctga ctctcctcca tttcgcgcag tacctggtgc acctgcttga
     5281 gaaatggtag cgacaacgat cccgactgcg cattgtcgag aaagcggcga ccgctgatat
     5341 cgcggagcag catattggcg tagcgtctga tcacgagcca cgcggccagc accaggaaca
     5401 gcgccacgat aaccaaggtg atgcacgcga gtgtgatcag gaaatcgcgc gccttgtcct
     5461 gtcggcgatc gacaaagctc agatcattga cgatctccac gcggtagggg gcctgtcctt
     5521 gtggcttctc atcgaacgcc gagaccagca ccgagcccca cgacgcacgc atgacatcgc
     5581 tctgtccatc ggcaagcttc gccgtactgt cgcagctgac ggctccgggg acccgctcgg
     5641 tcttgaactc agtccagcca tccgcgccac agatcagcaa tcccaccagg cgctgatccg
     5701 cggtgatctt ggaggcgtat tttcgcagcc cgggttgatc gtgtgtagcc aggagagcag
     5761 gcagggtttc ctcgatcgaa ctgatcacca ggcgcgcgcg cagctccacg tcggattgga
     5821 accaattggt aagcaggcga tcgatgtacg gcagaccgag gtacgcgaca acgccggccg
     5881 ccagcagcag cggaagaacg gtgcgaaaaa tgattgtgcg gtacattggt ctggtaggtg
     5941 ggttgcatcg acgctagccg cccgagctcg ctgccgtcaa tgcgacaaac gcgcaatcac
     6001 ggctgaaatt ttttcaattt cgactaatct atgcgcctgt gaccacattg cagattgtgt
     6061 gtttggcatt ggccgtatcg gcgctgacgc tggcgctgat ctttggcgtg tttcgcagcg
     6121 ggaagccgcc ggcggtcgcg gcagctccgc cgtcgacacc agcgcagtcg gctagacctg
     6181 ctgcggccgt agcacccgcg gtaccggcga cccaggatga gcctcccgaa gagctgaatg
     6241 cgcgcttcga ggccacgcac cggctgcagc tgctggcgtt gagttctgag gccgcgcctc
     6301 ctgccgatag tccggttcac gaagagatcg cctcgaaggt gcgctggacg ctcgccacca
     6361 ttaccgataa acccaactat gcgccgcgtc gcccgctgct gctgcctagg ctggtgcagg
     6421 cgatgaacaa cgacgacgtc tcccggcgtg agctggcgcg catcatcgct tccgatccgg
     6481 gactggcggc cgggctgttt cgcctggcca acagtccgtt ctatcgtgtc tctccaactc
     6541 cgatcgaaag tctcgaccga gcggtcgcgc tgctcggtat cgagggcatg cgctcattgg
     6601 tcgctgccgc cctgatgcag ccggtgttca aagtagatgg cgggggattc ccccgattcg
     6661 gcgacatcgt ctggcaacac acgctggcat cagcttccgc ggccgaatcg cacgcagtca
     6721 tgctcgagac caccgatcca ttcgctgcgc aactactggc cttgatgatg gggctatcca
     6781 cgatcgtggt gttccgggtc gcgattgatg aataccaggg gcggcatctg gcccctcacc
     6841 ccagagtgtt gagggcgctg atcgactcgc agtccgctcc ggttgcgcgc cagatcgcgg
     6901 cgagctggca gttgtccgag cgtgtcaatg ctgcgctggt cgatcaaacc gccccgcagg
     6961 gctccaagct gacgtcgttg gggcgatcac tgcagtttgg ccgctttctc ggcgcactgg
     7021 cggtactgca tcagcaacag gtgctcgacg atgaggcggt cagcgcggca ttgcgtaccg
     7081 gcggaagcct cgcgcccgcc tacgagcgca tctgggccaa gcgcgcggct ctgcagaacg
     7141 aggcgcatcg cccatagtgg ctacagcagc gatggcgcgt ttgcgatccg atcgcggacc
     7201 gcggccgtca gcgtcagcga atgctgccgc gccgcctggt catacacgtg cacattcacc
     7261 atcagttcgt cggcctgagt cagacgcagg aaaccgcgta agctgtgctc gacgctggct
     7321 gccgagccag caaacgtgta ccgcatcgcg tgctcgacca tcgtgcgctc ccccggcgac
     7381 cagaacgcgt tcatgtcgtc tctgggagcg ggcagctttc ctggccgccc gcggcgcaga
     7441 ttcataaagg cctgttgatg tgagctcagc agccagcgtg cttcctcgtc ggtatcggcg
     7501 acgatgacat tgatacaggg catcgcatac ggcttgctga gctgattcga ggattcgaag
     7561 cgctcgcgat agatcgatag cgccggcagt agcaggtccg gtgcgaaatg cgcagcgaac
     7621 gaaaaaggca gccccagcat ggccgcaagc tgcgcgccaa acatactgga cccgagcaac
     7681 cagatcggca ccttcagtcc cgctcccgga actgcgcgca atgtttgcgc cgatgagcct
     7741 tcaaagtagt gctgcaattc ctgcacatcg cgcggaaact cttcgccgct ggagaccaga
     7801 tcgcgcctga gcgcccgcgc ggtttccggg tcggttcccg gcgcgcggcc aagtcccaga
     7861 tcgatccgat ccccgtagag tgacgccaag gttccgaact gctcagcgat gaccagcggc
     7921 gaatggttcg gcagcatgat tccgccggag cctacgcgga tgctgcgagt acctgcagca
     7981 acatgtccaa tgacgatcgc agtggcggcg ctggcgatgc cgggcatatt gtgatgctcg
     8041 gccagccaga agcgcttgaa tccgagaggc tcgacatgac gcgccagcgc cagagtgtta
     8101 cgcaacgcct cggcggccgt agccccttcg acgatcggcg acaaatccag caccgagaat
     8161 ggaatcatgc ggtcgatgat cgcacgtcct tacggcggtg ggatgatcgt gaccgcgtga
     8221 agtctcagat cgcgcgaagc cgcgcccaca tagaccgtcc gcgcgccgac tgcggtcacc
     8281 cacctggccc gggcggcgga ccagtactgc aaggcgcgta cctgcgcatg caacgtgagc
     8341 cgtcttgatt caccggcggc tagatgcacc cgttggaacg cggccagcgc cctcggggca
     8401 aactgcgtac ccgggtcact gacggctcgt tcgggcgcac ccagatacac ttgtaccact
     8461 tcatcgctgg cgtaggcgcc ggtattgcgt agttgcacac tgatatccag gcctccgtcc
     8521 gcggccgcgt gcgcccacag atccgaatac tcaaagcgcg agtagctcag gccgaagcca
     8581 aacggaaaca gcggttccag cccctgctga tcaaaccagc gatagccgat gaagataccc
     8641 tcggaatact gtgtgcgacc attcaccccg aacgaggatc gctccggatg tgcaggatcg
     8701 ttggcaacgt tttgatcgag ccgctgcggc cacgtgaacg gcagccgtcc gccagggctg
     8761 gttcggccca gtaacagatt cgcggtggct ggaccaccct catcgccggg ccaccacatc
     8821 agaagaacag ccttgacctt gttcagccac ggcatcgcca ccggttcgct gaggttcagc
     8881 accacgatgg tgttcgggtt gacggccgcg acatcggcaa tcagctgatc ctgatcacca
     8941 ggcagaccaa atgccggacg acctcgactc caggcaaaga cgatcgcctt gctggtgtgc
     9001 gccgcggctt cgatcgcttg ctgatagtcc gcattgcgtt gctcgggcgt cacccaggca
     9061 aggcgcacct gcaccggctg tccggcccgc tcgcccgtca catgcacagt gaccgggtga
     9121 gcgcccgccg tcagcgtcat ctcacgccgc acatcgtcga gcccatcgag gctcggcagt
     9181 acattgtcct gcccgggctg caccagattg ccgtgcagat ccagcgcgcc gctgtgagca
     9241 acccgcctgc cgtcgacctc catcgtcgcg ctcgcaccga gcacctgcag atacagccga
     9301 tagcgaccgt tcactgggat cggcagcgtg ccgctccagc tgaacgatga gcccggggcc
     9361 agcgcgttac cgcgtgcgcg cgtgaactcc agactcgggt cctgcccgat gacctgaccc
     9421 tgcgtatcca ggcgttcaaa gctgacagga acggtgcggc cgttcatatc atcggccacc
     9481 gcccgctcga tgcgagcgcc ggcgtcaggg cgggcacccg ccaaccgtgc gatcgcctcc
     9541 gccggactcg tctgacgctc cacacggccc agtgccttct cgcctgcctc gccggccgcg
     9601 atgtcctgca gagccccggg accgatcagc gcgagggaat gaaggtctgc cgcgatcagc
     9661 ggcaatgcgt tcactgagtt cttcagcagg accgcggcgt cttcgctggt tttcagtacg
     9721 gtttcggcat tggcgagctc ggcgcgcccg acctgcacac ctctgaccgg ggcggcgccc
     9781 ttccggtcga gcaacccaaa ccgctgcatc tgcatgagga tgcgtcccgc agcacgcgtg
     9841 atcgtctgct cgctgaccgt accgcgctcc agcgcgctga gcatgccgat cggccgatcc
     9901 atcggcacgg tcagaacgaa cgccggcgtg tccggggcgt actcctcggg gattggactg
     9961 atttcaggac cacgcagcgg cttgcgtagg ctccctggtc gcggcagctg cgcctcgaaa
    10021 taggaatcca tcgtggtgcc gctgcccggc atttccaggt catcgcccgc ttcgatgaag
    10081 gatgtaccgt gtagcgcgcc ccagtccgag gtgacgaagc cgccgaactt gagctcgtcg
    10141 cgcaacacgc ccgtcagcag ggcgcgattg ccacaagcgt aggcgccatt gatgacattg
    10201 taggagcaca tgatggagga gacgcgcgca cgaacggccg catcgaacgg cgccagatag
    10261 atctcatgca gggcttgagc gttcatcgtg acatcgttgg caccgtcgta gccgatgaag
    10321 tgtttcgcct gagccatgac gccctcgccc tgtatgcccc gaatcatcgc ggctccgatc
    10381 atacccgtca gcagcgggtc ctcaccgaag gtgttgtagg cccgcgcgaa agtctgatcg
    10441 cgatgcagat cgatgaaggg ctgcagggcg acgtccacgc cgaggttcct ggcatcccgc
    10501 ccgatcaccg cgccgttggc gcgcgcatcc tccaggctga aggtcgcggc aagtcccatc
    10561 gtcgcggtca gggccgttgc cgggtagcgt gtcagcacgc ctggggggcc atcggcgaaa
    10621 cgcaggggcg gaatccctaa gcgaggcact cccggcaggt agccggcctc accctgatcg
    10681 gtggcagggt cttccggtac cccgtgcagg agcgagatct tctcttccag cgtcatctgc
    10741 gtgagcaatc gctccacgcg cgcactctcg ggctgatgag gagggtgatc gcgcaatgtg
    10801 caggcaatcg gacaaagcgc gaccgcgccg ataacggcga ctcttcgatt catcgatacc
    10861 ctttctggcg ctgccagcga cggcagctcg agcggcgcca gtgaagggta tcgtcagatc
    10921 agaccaccgc gctgagcaga tggcctgcgc gggcctgtac cccttgctgc gccagacgtt
    10981 gcgccaggct ctgggcgctg tgcgccgcgg ttgcggaatt gagcgcggcg acattcgtgc
    11041 tgctggcggc cagtttaaga ttctgcagca gctggttcaa cgtcgcctcg acgttgctag
    11101 ccgatgcggc gcccgccgat gtcgtggtgc ccgctgtgtt gaccgctgtt gcgccggcgg
    11161 tgctggcgga ggcagcgctg cttggcccgg tagtgccagc ggccagctcg tcgagctgct
    11221 gcaacagcgc actgagggcg agtccgccgt gtcggtgatg cagcccgttc acggcctgca
    11281 gtccgggatt cgccgtcgcc gcgacgcctc gggtattgcc ggccggattg gctgaattga
    11341 tctgcgaaat cagattctca aacgcgctcg caagctgcgt accgttggtg ggcgtcgtgc
    11401 tgcttcgcgc agcgccggcg ccggcgccgg tcaagtttga cgatgatgtg ccgttaacct
    11461 gactagccag gctgccctgg ttgaaccagg tgctcactgt ggggatcaat agtcccaaca
    11521 taaattgact cctcgctctt aacgctcaag acgatcgttt gccacgtggc agcgtgttgc
    11581 cggcacagac ttgccctccc acttgctctc gaacccggct gtgatggctc gttctgccaa
    11641 tcgcatcaca gtctggcacc cgaaagcccg ttgaggccta cctcccggcg gacccattca
    11701 ccgcacgcat ctgaagcaat gcgaaaacaa tgccaagcgt gaatgccata atcgcgcgat
    11761 gcagcgtggg attcgcagct acgtcatccg aaccggccgc atcacggacg cgcagcgccg
    11821 cgcgcgtgag cagcactggc cgtactttgg gatcgaattc cagcctcagc tactggatct
    11881 ggagcactgc ttcggacgcc gcgccgctcg cacgctagag atcggctttg gcaacggcga
    11941 gcacttactc gagcgggcgc agtgcagtcc cgagcgtgat tttctgggcg tcgaggtgca
    12001 tgagccgggg atcggtcatc tgctgctggc cgccgcccgg gcgcagctcc cgaacgtgcg
    12061 cgtgatcgcg cacgacgcgg tggaggttct gcaacaccag atcgcacccg acactctgga
    12121 tgaggtacag ttgctgttcc cggacccgtg gcccaaaaag cgccatcaca aacgtcgcat
    12181 cgtccaaccg gacttcgcgt tgctggtcgc gagccgactg agccccgagg ggcgctttca
    12241 tctcgcgacc gactggaccc cctacgccga acagatgctg aacgtgctga acgactgccc
    12301 cttgctggtg aactgcgcaa cggatctcgg cttcgtgggt cccgaagagg tgatgacccg
    12361 gcaggcgacg cgattccaac gtcgcggcga gcgcctgggc cattctgtgc acgagctgct
    12421 ctatcgccgt cggtagcacc ggagctcacg attgcagagc ccccggggct cgcgacttcc
    12481 gggaatccta cagcggccac aatgagtgaa atcggtgtga cactgttcgc gcatagataa
    12541 gaaatttgag aagtcgcgca ttcatttgat gctctgcacg atcaaattca tcttgaatga
    12601 gtagtgccgc aggagcattc tcaggacgcg gactaaacgg gagctctggc aatgagtgcg
    12661 caacccgaca tcggcgaatg gtatcgaatt cgcggcggtg atcagttcga agtggtggcc
    12721 atcgatgaag acgatggcac gatcgaagtg cagtacttcg atggcacggt tgaagagatc
    12781 gacatcgggg actgggaagc gcaacacgcc aacggtgaga tcgcagaggc cgaaacccag
    12841 gatgattgga gcggttcggt cgacggtgac gacgacaacg aggagttcgc cctcaccagt
    12901 cacttcgaca gtcgcccgca cacctcaggg ggactggacg gcctcgatct gttcgagtcg
    12961 ccggattact ttcagtagcg acgccatcga gcgattcgat caggcatagc gctgcgcgag
    13021 ctcatcgggg tttgcctcgt ccgccagcaa cacatatccg gcttccactc gcaccggcaa
    13081 cgcaatcaac gacaaccctg agcaaggacc ggcgacgcag tagccggtgc tcttctcaaa
    13141 aatggcgccg tgcacgtagc actggatcat gctgccgtcg tgcgtcagaa attggtgccg
    13201 caggtagttg agcgggtagc tcaagtgcgc gcatcgattc acgtacgcgc gcacgccatc
    13261 acccacctgc acgacgaatc cgcgcagtgg ccaatcgccc ctgcccagcc gaaactcacg
    13321 acagccgcct tccagatccg cgatccggca cagcacgcgc gccagatcga tttccgtcgc
    13381 cgtcatgtgt gctgccggtc caacgtctcg atcttgtggc gcgtgaacca gacgatcgcg
    13441 agcccaggaa gggcaatgag gaagctcacg acgaaatagg cataccaacc cgcgtgatcg
    13501 gcgatccagc ctgccggata gcccagagcg tagcgcggca gcagtgcgat tgaggacagc
    13561 aaggcgtatt ggaaggcgct gtagcgcagg tcgcacaagg ccatgatcaa cgcgatgagg
    13621 gctatgttgc ccatggcacc cgccacatgt tcggtcagca cggcccctac catcaacgga
    13681 aaattcttac ccgttgccgc cagagcgcag tacagcaggt tcgtgagcgc ctgcgacgcg
    13741 ccaaatatca gcatcgagcg cagcagcccc agtctcacca tcatgatgcc accgagaatg
    13801 gcgcctccga tcgcccccgc agttaacagg ggtttgacga ccagcgcgac ttcctgttta
    13861 gagaacccga catcaagcat gaaaggggtg aatagtttgt ttgcgaaagc atcgccaatc
    13921 ttgaagagca gcacgacgac gagcagcgct ttgatacctg gccctgccag caattcccgc
    13981 aacggggtaa tcacagactc ccgaagggaa ctcggagaag cgcgtgcgct ggctggctcc
    14041 ggcgcgaacc aagtgctgaa acaaaacaga atcatgatgc ccgcgagaat tagaaacgcc
    14101 ggtcgccagc cgaaatgttc cgcaacgaga agagcgaacg cggatgcaag ccacgctgcg
    14161 gatcgatagc cgagattggt taccgcggca gcgggcccgc gctcactcgg agttgaaacg
    14221 tcggttctcc aggcgtcgat gccaatatcc tgggtcgcgg agaagaacac gattgccacg
    14281 gcgcagactg cgattggaac cagcgtttgc gcggggtctt gcaaagcaaa cagcgcgatt
    14341 gcggcagcca gcaggatctg catcaccaga atccacccac gacgccgacc caacagtggc
    14401 aacggatacc gatcgatgag cggcgcccat agaaatttca gcaggtacgg caacgccacg
    14461 tacgacatca cgccgatctt ggtgagcgag acagcggaat ccttgagcca cgcctgcagc
    14521 gcgctctcgg tcaactggtt ggggaaaccc gacgccgcgc ccaacagcat gatggctagc
    14581 atctttggac ttgccagaac ctcccgaagc ggcggtgcct tcgggcgctg cggggcgggt
    14641 ggcggcgtca aagccgatag tccatgatca atggtgcgtg gtccgagaat cggggctctt
    14701 tgtgaatatg ggccttgcgc ggcgtcccgg cgaggccggg actgaggatc tgatagtcaa
    14761 ttctccatcc gacgttcttg gcgtacgcgg caccccgatt ggaccaccag gtatagtgat
    14821 ccggctcggg caccagcgag cggaaagcgt cgatgtagcg ctccgatccg agcagttgat
    14881 ccatccatgc ccgctcctcg ggcaggaagc cggagttttt ctgattcgag cgccagttct
    14941 taagatcaat ctgcttgtgc gcgatgttcc agtcaccgca cagaatgtat tcccgtcggg
    15001 atcgccgcag ctttcgcagg tgcggcatga aggcctcgag gaatcggaac ttcgaggcct
    15061 ggcgctcagg tccggccgat ccggacggca ggtacaacga caccacgcat agtttgccga
    15121 attgcagctc caggtagcga ccctcgcgat cgaattcgtc gatcttgaat cctcgcacca
    15181 cgcggtccgg ttcgcgccgc gataacaccg cgacgcccgc gtagccagga cgttccgcag
    15241 cgtatagatg cttcaggtat ccgggcacct gatgggtatc ctcccattga tgctcgagcg
    15301 ctcgcgtctc ctgcagacag acgacatcag catgttgccg ccccagccag tcgaacagac
    15361 cacgcttgcc cgccgagcgt atgccgttga cgttgagcgt gatgattctc atgcgggaat
    15421 gatacccttc ctccggtgct ggactatcaa cgacagttcc tcgatatggc gctcgcgcgg
    15481 caggctctgc gctttggcca gttcacactg aagtccggca gagtcagtcc ttacttcttc
    15541 aacgccggcc tgttcaacga cggccagtcg ctcgccactc tggggcgctg ctacgccggc
    15601 gcgctgctgc attcgcagtt gcagttcgat ctgctgttcg ggccggccta taaggggatc
    15661 ccgctcgtcg ccagcaccgc ggtggcaatc gccgacgccc agcgccgcaa cgtaccctgg
    15721 gccttcaacc gtaaggaggc gaaggatcac ggcgaagccg ggctgatcgt cggcagccca
    15781 ctgcagggtc gggtggtgat cctggatgac gtcatcaccg ccggtaccgc gatccgcgaa
    15841 tccatcgaac tgatccgcca ggccggagca gaggcggtag gtgtcgcact ggctctggat
    15901 cgtcaggagc gcgggcagac cgagcgctcc gcggtacagg aagtcgagag cctctatgga
    15961 gtcaaatgcg tgacgatcct cacgttcagt gaactgatta cggcgctcga ggagcgcgct
    16021 gcgaccgttc acggttccgc ggcaggctat ccggccccgg cgcccacgat tgagcaactc
    16081 gcggcgatgc gtacctatcg gctcgaatac ggcgtcacat cccagacgcc gtaaacttgc
    16141 acaatggggg cggcagaacc gacaatccgc cgaaaggtga gcattcctac catgcaccgc
    16201 gggtggtcca tcctcttgtc cagtctggcg ctcggagcgc taggcgtcgc ctatgccagt
    16261 cagtccggcc cggtttacaa gtggaccgac gaaaaaggta tcgtccatta tggcgatagc
    16321 gtgccccccg aatacgcgca gggcgagcgc tcggtgctga attcgcacgg cgttgaggtc
    16381 ggtcatgtcg aagggaccaa caacgcgaca gcgatggcgg agagagcccg cacggccgaa
    16441 gccgcgaagc tcagagccca gcacgatcag ttcctgctgt cgacctatct atcaaccaag
    16501 gacatcgaac agctgcggga cgaacgcctc ggccagatcg acggtcagat caaagcttcc
    16561 aacatctaca tcgacgggct cggcactcgc atgctcgcgc ttgaagatcg cgccacccac
    16621 ttcaaaccgt acagcagcga ccccaaggct cgtcgcatgc cggacgatct cgccgaggag
    16681 ctggtaaggg tgtcgaacga gacccgcagt cagcatcagg cgctcgacgc caagcacaga
    16741 caacagagcg atatgcgtgc tcaattcgac gccgatatcg cccgctttcg cgagctgact
    16801 gcaaagccca agtcttgaat cagcgccgcc tcactgccgg ccggagtgcc cgaaccctcc
    16861 ggcaccgcgt gcgctggcac tgaagtcctg cacgatatcg agcgcaacct gcaccaccgg
    16921 cacgacgacc aactgcgcga tacgctctcc gggctgaatc cgataactgt cgctgccacg
    16981 attccagcac gacaccatca acggcccctg at
//
LOCUS       DATMRM010000510         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3399124, whole genome shotgun sequence.
ACCESSION   DATMRM010000510 DATMRM010000000
VERSION     DATMRM010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3399124"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="VNZ06_15595"
     CDS             <1..1084
                     /locus_tag="VNZ06_15595"
                     /inference="COORDINATES: protein motif:HMM:NF024896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWW22225.1"
                     /translation="SGPRAAVLIAIKGVSSTTVRFLSALRSQRYDNYRLIFALESAVD
                     PAYALVMQFKAELAGERTVDVVLAGVSTECSQKVHNLLAALRMLRQEDRIVVFADADI
                     VPVDTWLAQLIRPVAHGETASACGYRWQLAVKREWPSLILAAADWSVATAARSRMWNL
                     CWGGSLALGREALEQLDLPRVWARAASDDLTLTRALRAKALRIYVPPFVLVPSPISYD
                     WGNLFRFMHRQYLLIRVYAPRHWLFAAVTLAIPAFGAMLAIALACRGQWWSLTFTVAS
                     ILLLQIRLSVRRSIAYLVLPPADIPTVLTTVKFARWAWPLIHLIHTSALASSAFGRRF
                     SWAGTRYELDGLKVHVRGEPREVKNV"
     gene            1240..>2315
                     /locus_tag="VNZ06_15600"
     CDS             1240..>2315
                     /locus_tag="VNZ06_15600"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HWW22226.1"
                     /translation="MPSNHVRAVACVAAIALSSCTSILPTIGPSRHDIEHAKRQPNPG
                     AIEIIDINDELAHTLLAQRQQRLFSETLAGTPANPRTVGPGDVLEVSIWEAAPPTLFT
                     TNTTIGVMSTSHAVMLPEQTIDDEGDIMVPFAGRVPASGKTVEAIEREIVDRLAKKAN
                     QPEVLVQRTHNASANATVVGEVTTSTRVPLQSGSEHLLDALAAAGGVKQPVNKTTIQV
                     TRANSVYSLPLETIIRDPRQNIALVPGDVVTALFQPYSFTALGATGRVDEVNFETQGI
                     TLAQALARANGLIDARSNAKGVFVFRLEPPNALTWPHPPKYTTPQGLVPTVFRIDMTD
                     PQSLFLIQDFWIENRDILYVSNAP"
BASE COUNT          438 a          722 c          705 g          450 t
ORIGIN      
        1 ctcagggccg cgtgcagccg tattgattgc gatcaaaggc gtcagcagca cgacggtgcg
       61 ctttctgagc gcgctgcgct cacagcgcta tgacaactac cgactgatct tcgcgctgga
      121 gtctgccgtt gatcccgcct acgcactcgt catgcagttc aaggccgaac ttgcgggcga
      181 gcggacagtg gatgtggtgc tcgccggcgt cagcaccgaa tgcagccaga aggtccacaa
      241 cctgctggcg gcgctgcgca tgctgcggca ggaggatcgg atcgtcgtgt tcgcggatgc
      301 cgacatcgtt cctgtggaca catggctggc gcagctcatc cgcccggttg cgcatggcga
      361 aaccgcttcg gcatgcggct atcgctggca gttggcagtc aagcgcgaat ggccaagcct
      421 gattctggcg gcagcggatt ggtccgttgc gacggctgcg cgctcaagaa tgtggaacct
      481 gtgctggggc ggctcgctcg ccctgggtcg cgaggcgctg gaacaactgg atcttccgcg
      541 cgtgtgggcg cgagccgcct cggacgacct gacgctgacc cgtgcgctcc gcgccaaggc
      601 gctgcgcatt tacgtgccac cattcgtatt ggtgcctagc ccgatatcgt acgactgggg
      661 caacctgttt cgtttcatgc atcgtcagta tctgctcatt cgggtgtacg cgccgcggca
      721 ctggctgttc gctgccgtaa cactggcgat tcccgcgttc ggagcgatgt tggcgattgc
      781 gctcgcgtgt cgcggacagt ggtggtcgct cacttttacg gtcgcgagca tcctgctgct
      841 ccagattcgg ctcagcgttc ggcgctcgat cgcctatctc gtgctgccgc cggcggacat
      901 tcccaccgtg ctcacgactg ttaaattcgc gcgctgggcg tggccgttga tccacctgat
      961 ccacacctca gcgcttgcaa gcagcgcctt cggcaggcga ttttcctggg ccggaacacg
     1021 ctacgaactc gatggtctca aggtccatgt gcgtggggaa ccgcgagagg tcaagaacgt
     1081 ttagagcctt gcaaatacag caaaaatcat cgaccatcac gtcggcagtt gcatgagaag
     1141 atggaccgac gacatagcga ctgccccagt ggcgtccctg cgccaacccc tgggtttaga
     1201 agattttacg ttagcgtcgg ctgatgtcag gccttttaaa tgccaagtaa tcatgtccgc
     1261 gccgtcgcgt gcgtcgcggc aattgcactt tcgagctgta ccagcattct tccgaccatc
     1321 ggtccgagtc gtcatgacat cgaacatgcg aaacggcagc ccaatccggg cgcgatcgag
     1381 atcatcgaca ttaacgacga gctggcacat acgctgctgg cgcagcgtca acagcggctg
     1441 ttctcggaga ctctggcggg cacgcccgcc aaccctcgca ccgtgggtcc cggcgacgtc
     1501 ctggaggtgt caatctggga agcggcaccg ccaacgctgt tcacgaccaa caccacgatt
     1561 ggcgtgatgt ccacatcgca cgcggtaatg ctgccagagc agacgataga cgatgaaggc
     1621 gacatcatgg tgcccttcgc gggccgggtc ccggcatcgg gcaagacagt cgaggcgatt
     1681 gagcgcgaga tcgtcgaccg tctggccaag aaggccaacc agccggaagt gctggtgcaa
     1741 cgcactcaca acgcatcggc gaatgcgact gtagtcggtg aggtcactac cagtacccga
     1801 gtccctctgc agtcaggcag cgaacatctg ctggatgcgc tggctgcggc cgggggcgtt
     1861 aagcagcccg tcaacaagac gaccatacag gtgacgcgcg ccaacagcgt gtattcactg
     1921 ccgcttgaga cgatcattcg cgatccacgg cagaacattg cgttggtgcc gggcgatgtc
     1981 gtgaccgctc tcttccagcc ctacagcttc acggccctcg gagcgaccgg gcgggtggac
     2041 gaagtcaact tcgagaccca gggcatcacg ctggcccagg cgctggcacg cgcaaacgga
     2101 ttgatcgatg cccggtccaa tgccaagggc gtgttcgtat tccgcctgga gccaccgaat
     2161 gcgctgacct ggccgcaccc tcccaaatac acgaccccgc aggggctcgt gccaacggtg
     2221 tttcgcatcg atatgaccga cccgcaaagc ctgtttctaa tccaggattt ctggatcgag
     2281 aaccgcgaca tcctctacgt gtccaatgct ccgat
//
LOCUS       DATMRM010000511         9135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_298492, whole genome shotgun sequence.
ACCESSION   DATMRM010000511 DATMRM010000000
VERSION     DATMRM010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9135
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298492"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /gene="rsmI"
                     /locus_tag="VNZ06_15605"
     CDS             <1..663
                     /gene="rsmI"
                     /locus_tag="VNZ06_15605"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HWW22227.1"
                     /translation="EAVVQQLQSGKVIALVSDAGTPLLSDPGFALVRAAVAAGIEVRA
                     IPGPSALTAALSVAGLATDRFTFEGFPSSRQTERRAAFERLSHEPRTLVFFEAPHRIA
                     ATLADLAQIFGAERQAVVARELTKMHESIYRGTLSGLHELSISNVDMARGEITLIVAG
                     AAKQGANQGDHELMARSLRLLLPELPPARAAAIASQLAGCKRSEAYELALRMRVDLKS
                     NN"
     gene            707..1082
                     /gene="rnpB"
                     /locus_tag="VNZ06_15610"
     ncRNA           707..1082
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VNZ06_15610"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1300..1755
                     /gene="mraZ"
                     /locus_tag="VNZ06_15615"
     CDS             1300..1755
                     /gene="mraZ"
                     /locus_tag="VNZ06_15615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HWW22228.1"
                     /translation="MFRGATKIALDDKGRMVVPVRYRERLAELSAGQLIVTVNLDGEC
                     LLIYPAADWEPVEREVMKMPAFHPQTSRLRRLMVGYATELTLDGHGRVLLPSELREFA
                     QIERNAMLIGQGNRFELWQEARWTERSNFWLQSGTPGTDLPPEFGSFSL"
     gene            1789..2715
                     /gene="rsmH"
                     /locus_tag="VNZ06_15620"
     CDS             1789..2715
                     /gene="rsmH"
                     /locus_tag="VNZ06_15620"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015271715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWW22229.1"
                     /translation="MDEHTPVLVEEVVSALALRPAGLYVDATFGRGGHTSRILSALAD
                     GGEVIAIDRDPQAIAAGRARFAGQARLHLVQGEFAELGELVRSQTARTQCDGILFDLG
                     VSSPQLDDPARGFSFSKDGPLDLRMDPTRGEPASLWLEHASLDEIRHVIATLGEERFA
                     KRISQEIVRARAHAPLRRTLELAALVAGCVRTREPGKHPATRTFQALRMFINDELGQI
                     RRGLEQTLSLLAPGGRLAVISFHSLEDRLVKQFIRQHSELAPELARLPIAPAGVALPL
                     RRIGRKLRASAAEVAANPRARSALLRVAERLA"
     gene            2712..2993
                     /gene="ftsL"
                     /locus_tag="VNZ06_15625"
     CDS             2712..2993
                     /gene="ftsL"
                     /locus_tag="VNZ06_15625"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HWW22230.1"
                     /translation="MSVGRKLLLFALPALWCAVLGASAGAIYAKYRARELFVELERLS
                     SMRDDLDAEWGRLQLEQSAWSNYGYVERVASERLHMSIPDSREIEILSP"
     gene            2990..4732
                     /locus_tag="VNZ06_15630"
     CDS             2990..4732
                     /locus_tag="VNZ06_15630"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWW22231.1"
                     /translation="MRGLGHSSKERSAAQSFRRRQWLLFGLLIAGAAGLVGRAVDLQL
                     VAHGFLAKQGDARYSRVATIIAHRGNIVDRNGESLAISTPVDSVWVNPQELAGSIEQL
                     PHLANALSVDRTELTRHISSNMGRDFLYVARGMTPDDAQRVKGLNLPGVYLTGGYRRY
                     YPAGEVTGHVLGFTSIDDVGQEGTELSFDNWLAGEDGAKRVIQDSRGRKVEDVESIRP
                     VRPGRDLTLSLDLRIQYIAYRELKSALVENRARAGSMVVIDIETGEVLAMVNQPAYNP
                     NDRSQMLPGTYRNRAVTDLFEPGSSIKPFIVASALSSGKFNTDSVIDTSPGFIQVGER
                     TFPDEHNLGVIGLATVLAKSSNVGMIKLALQLEPQQIWNTLHRFGFGQVTASGFPGEA
                     AGVFTNYSHWRALSIATLSHGYGISVSALQLAQGYAALGALGVRRPVSMLKVDLPPAG
                     ERVMDESVCRSMLTLLEAVTTQEGATGVLAKIPGYRVAGKTGTAYIAVNGSYSKDQYT
                     SVFAGVAPASNPRLAAVVVIDDPTAGKHHGGDVSAPVFSAVVGGALRLLAVAPDELTA
                     PTTPLVHGQRVAQR"
     gene            4735..6267
                     /locus_tag="VNZ06_15635"
     CDS             4735..6267
                     /locus_tag="VNZ06_15635"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HWW22232.1"
                     /translation="MTAQPTWRPLKAVLGGLVDIPDRLEVSDITQDSRSVIPGAAFLA
                     CQGRTQHGLKFVDAAVSAGARAVLWEPAPGVAAPVVDGSIVVRAVPNLHAQLGYIADR
                     FFDAPSAGMMVAAITGTNGKTTCAWLLAQALNLCGRSAAYIGTLGFGTPGVMHTLEQT
                     TPDAVTLQRALATLRAAGMDSIAMEVSSHALDQNRCAGMRMHSAVFTNLSRDHLDYHG
                     DMDSYGAAKAKLFNWPTLAARVINVDDPFGQELARERLQGAGAVQSRLFLTSQHSNEW
                     LTSGADFVYTEGVRASAHGLELDVESSRGETLLHSHLLGDFNVDNLLTVLATLLAWDI
                     PLDQARMALERCSAPPGRMQPDGGGALPLVLVDYAHTPDALSKALHAARAHCDGRLWC
                     VFGCGGERDVGKRGLMGQVAAQLADEIVVTDDNPRRENPDRIVAAIMEGVIAAGAGWR
                     VQVNHDRAQAIRAAVSKAHAGDVVLVAGKGHEDYQLIGNERREFSDAAVVHQILAEQA
                     PQ"
     gene            6264..7622
                     /gene="murF"
                     /locus_tag="VNZ06_15640"
     CDS             6264..7622
                     /gene="murF"
                     /locus_tag="VNZ06_15640"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HWW22233.1"
                     /translation="MIRTLQQFAAAAHGRLLGEDREFGELAIDSRKLSAGDLFAALRG
                     EQLDGHDYVAAAATAGAAGAIVQRKLKVALPQIVVSNVATAMAQAARAARSQFKGPVI
                     GVAGSNGKTTVKEMLSAILSQLGPCLSTRGNLNNHLGVPMTLLRLQDTHRSAVIEMGA
                     NRRGDVEQLVQIARPGIGLITNAGAEHLEGFGSLEGAARAEGEMVAELPSSGIAVINA
                     DDAYASLWRAATSAQIVTFGLRQEADFHPRSVHFDVNAEGFITRFCLCSRSGEVDVAL
                     AVAGRHNVLNALAAAAAATNAGVTLEQVASGLSSMRAAKGRLQFKRTQAGAWLIDDSY
                     NANPSSARAALEVLSELPGRRWMVLGDMAELGAFADSSHREIGELAHNLGVERLYTFG
                     NLAALAAEAFGPGAERFADAAALAQALNAALTPDVRLLIKGSRVNRLERVVEALSRAE
                     TA"
     gene            7640..8725
                     /gene="mraY"
                     /locus_tag="VNZ06_15645"
     CDS             7640..8725
                     /gene="mraY"
                     /locus_tag="VNZ06_15645"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWW22234.1"
                     /translation="MLFWIAKLLSVHYTAFHVFSYLTLRAILAVMSALAMSLLIGPSM
                     IASLSRYQIGQVVRDDGPQSHLPKAGTPTMGGALIIVVIAVSTLLWADLRNRFVWITL
                     AVTVGFGLIGFYDDYLKLVVRNSKGLIPRKKYFYQSVLGIGAAVLLYRTAHAPAETDF
                     FLPFVKSVQLPLGGIGFCTLAYLMIVGMSNAVNLTDGLDGLAIMPAAMVAGALGVFAY
                     ASGNSIFANYLSIPAIPQAGELLVFCSALAGAGLGFLWFNSYPAQVFMGDIGALALGA
                     ALGVVATVVRQEIVALIMGGIFVLETASVILQVASFKLTGRRIFRMAPIHHHFELKGW
                     AEPKVIVRFWIISFLLVLAGLSTLKLR"
     gene            8722..>9135
                     /locus_tag="VNZ06_15650"
     CDS             8722..>9135
                     /locus_tag="VNZ06_15650"
                     /inference="COORDINATES: protein motif:HMM:NF045460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWW22235.1"
                     /translation="MSTAATKPLSAVVVGMGKSGYSAARYLLARGTRVAVTDSRAQPP
                     ELARLRSLGEQITMRVGGFDASLLEHADLVVVSPGVALSGAFFEQARARGLPMVGDIE
                     LFARAVRAPVAGITGTNGKSTVTTLLALMAQKSGLR"
BASE COUNT         1583 a         2777 c         3075 g         1700 t
ORIGIN      
        1 gaggcagtgg tgcaacagct gcagtccggc aaagtgatcg cgctggtgag cgacgccggt
       61 acgccgctgc tgtccgatcc gggcttcgcg ctggtgcgag ctgcggtcgc ggccggtatc
      121 gaggtacgcg cgatccccgg accctcggcc ctgacggccg cattgagtgt cgccggactg
      181 gccaccgatc gattcacctt tgagggattc ccctcatcgc gccagaccga gcggcgcgcc
      241 gcattcgaga ggctcagcca tgagccgcgc accctggtgt tcttcgaggc gccgcatcgg
      301 attgcggcca cgctggctga tctggcacag atctttggcg ccgaacggca ggcggtggtg
      361 gcacgcgaac tcaccaagat gcacgagagt atctatcgtg ggacgttgag cggtttgcac
      421 gaactgtcaa tcagcaatgt cgatatggca cgcggtgaga tcacgctgat cgtagccggc
      481 gccgcaaagc aaggcgcaaa ccaaggagac cacgaactca tggctcgctc gttacgattg
      541 ctgttgccgg agctgccacc ggcgcgtgcg gcggcgatcg catcgcagct tgccggctgc
      601 aagcgtagtg aggcttatga gctcgcgctg cgcatgcgcg tggatcttaa atctaacaac
      661 tgaagattga gtcgctcgtc gttacacagt atggtgataa gcgcggagag tcggctggac
      721 ggtcgctgcg tatcgttcga tgcgtggagg aaagtccggg cttcagcggg cgagggtgcc
      781 aggtaacacc tggggggcgc gagcccacgg aaagtgcaac agaaagcaaa ccgccgaagc
      841 gccggcaacg gcgctggtaa gggtgaaacg gtgcggtaag agcgcaccgc gtcagtggta
      901 acactcgacg gcacggcaaa ccccacctgg agcaaggcca aatagggaag tcgcgacctt
      961 gaaaggggtc gtagcgcgcg cccgcgcgtg cttccgggta ggccgctaga ggcgtgcggc
     1021 gacgtgcgtc ccagaggaat ggctgtcctc gacagaaccc ggcttacagg ctgactctcc
     1081 gccctccgac cttgccaatc tggtcgaact ctaagatcca agctcatgat cctgaaacgt
     1141 tcccagcctt acttggtcgt cgagtctggg tttatcgtct aagtctctac tgcgtctcaa
     1201 aaatttaccc gcaaatcatt gacacacaag gcctgcgatt tctatagtgg cagttagtgg
     1261 caaaaagtgg ggagggatgg gcaatctgcc cagaatccag tgttccgcgg cgcaacaaaa
     1321 atcgccttgg acgacaaggg acgcatggtc gtcccggtgc gttatcgcga gcgcctggct
     1381 gagctgtcgg ctgggcagct gatcgtgacg gtcaatctgg atggggaatg cctgctcatc
     1441 taccccgcgg ctgattggga accggtcgag cgcgaagtga tgaagatgcc cgctttccat
     1501 ccgcagacca gtcggctgcg acgcctgatg gtcggttatg cgacggagct gacgctcgat
     1561 gggcatgggc gcgtgctgct tccttccgag ttgcgcgaat tcgcccagat cgagcgcaac
     1621 gcaatgctga tcgggcaggg caatcgattc gaactatggc aggaggctcg ctggacggaa
     1681 aggagcaatt tctggctgca gagcggcacg ccgggaactg atctgccacc agagttcggt
     1741 tcgttttcgc tttgaccggc tgggaagcgt ggtcgggggc taggggagtt ggatgagcac
     1801 acgccggtgc ttgtcgaaga ggtggtatcg gccctggcgc tgcgcccggc cggactctac
     1861 gtcgacgcga cattcggacg cggtggacac acctcgcgga tcctcagcgc gctcgccgac
     1921 ggcggcgaag tcatcgcaat cgatcgcgac ccgcaggcga tcgcggccgg gcgcgcgcgc
     1981 tttgcgggcc aggctcgatt gcatcttgtg cagggtgagt tcgccgagct cggcgagctc
     2041 gtgcgctcgc agactgcgcg tacgcagtgc gacggaatcc tgttcgatct gggtgtctcc
     2101 tctccacagc tcgacgaccc cgcgcgcggt ttcagcttta gcaaggacgg tccgctcgat
     2161 ctgcgtatgg acccgacgcg cggcgagccc gcttccctgt ggcttgagca cgcgagcctg
     2221 gatgagatac gtcacgtgat cgcgaccctc ggagaagagc gcttcgcaaa gcgtatctcg
     2281 caagagatcg tgcgtgcgcg cgcgcacgcg ccgctgcgcc gcacgctcga gctggccgcg
     2341 ctggtggcgg gctgcgtgcg cacccgcgag ccgggcaagc atccggctac ccgtacgttt
     2401 caggcgctgc gaatgttcat caacgacgag ctcggccaga tccgccgcgg actcgagcag
     2461 acgttatcgc tgctggcccc tggcggacgt ctcgcggtga tcagcttcca ttcgctggaa
     2521 gaccgcttgg tcaaacagtt tatccgccag cactcggaac tcgcgcccga gctggcaagg
     2581 ctgccgatcg caccggccgg tgttgcgttg ccgctgcgtc gtatcggtcg caagctgcgc
     2641 gccagcgccg cggaagtcgc tgccaacccg cgcgcccgca gtgccctgct gcgtgtcgcc
     2701 gagagactgg catgagcgtt ggcagaaagc tgctgctgtt cgcgctgccg gcgttgtggt
     2761 gtgcggtgct cggcgccagt gccggcgcca tctacgccaa ataccgggcg cgcgaactgt
     2821 tcgtcgaact ggaacgtctg agcagcatgc gcgatgacct ggacgcggag tggggccggc
     2881 tgcagctcga acagagcgcc tggtccaact acggctacgt cgagcgcgtc gcgagcgagc
     2941 ggttgcacat gagtattccg gactcgcgtg agatcgagat tctgtcgcca tgagaggcct
     3001 ggggcacagt tcgaaggaac gtagcgcggc gcagtcgttt cgacggcgcc agtggctgct
     3061 gttcggactg ctgatcgctg gcgccgccgg gctggtcggc cgcgcggtcg atctgcagct
     3121 ggtcgcgcat ggctttctgg caaagcaggg ggatgcacgc tactcgcgcg tggcgacgat
     3181 catcgcccat cgcggcaaca tcgttgaccg caacggtgaa tcgctcgcga tcagcacgcc
     3241 ggtggactcg gtatgggtga atccgcagga gctggccggc agcatcgagc aattgccgca
     3301 tctggccaat gctctgagcg ttgatcgcac cgaactcacg cgccacatca gcagcaacat
     3361 gggccgcgac ttcctgtacg tggcgcgcgg catgacgccc gacgacgcgc agcgcgtcaa
     3421 agggctgaat ctcccgggcg tgtatctgac cggcggctat cgtcgatatt atccggccgg
     3481 agaagtgacc ggccacgtgc tcggtttcac cagcatcgac gatgtcggcc aggaaggtac
     3541 ggagctgtcg ttcgacaact ggctggcggg tgaagacggc gccaaacgcg tgatccagga
     3601 cagccgcggc cgcaaggtcg aggacgtgga gagcattcgt ccggtgcgcc ctggtcgcga
     3661 tctcacgttg agtctggacc tgcgcatcca gtacatcgcc taccgtgaac tgaagagtgc
     3721 gctggtcgag aatcgcgcac gcgccggctc gatggtggtg atcgatatcg agaccggcga
     3781 agtactggcg atggtcaatc agccagccta caacccgaac gatcgcagcc agatgctgcc
     3841 gggcacatac cgcaatcgcg cggtgaccga tctgttcgag ccgggctcga gcatcaagcc
     3901 attcatcgtc gcctcagcac tctcatccgg caagttcaat accgacagcg tgatcgatac
     3961 cagtccgggg ttcattcagg ttggggagcg tacctttcca gacgaacaca atctgggtgt
     4021 gatcgggttg gccacggtgc tcgcaaaatc ctcgaacgtc ggcatgatca agctcgcact
     4081 gcagctcgag ccgcagcaga tctggaatac gttgcatcgc tttggcttcg gtcaggtgac
     4141 ggcgagcggc ttcccgggcg aggcggccgg ggtgtttacc aactattcac attggcgcgc
     4201 gttgagcatc gcgacgctgt cgcatggcta cggcatttcc gtatcagccc tgcagctcgc
     4261 acaaggctac gccgcgctcg gcgcactcgg cgtgcgccgc ccggtatcca tgctgaaggt
     4321 agatctgccg ccagcgggcg agcgggtcat ggacgagtcg gtctgccgct cgatgctcac
     4381 gctgctggag gccgtgacca cacaggaggg cgcaaccggc gtgctcgcca agattccagg
     4441 atatcgcgtc gccggcaaga ccggcacggc ctacatcgcg gtgaacggca gttactccaa
     4501 ggatcagtat acctcggtgt ttgccggcgt cgctcccgca tccaatccgc gtctggccgc
     4561 ggttgtcgtg atagatgatc ccaccgcggg caaacaccac ggtggtgatg tttcggctcc
     4621 tgtgttttcc gccgtggtcg gtggggcgtt gcgattactc gcggtggcac ccgatgagct
     4681 gacagcgccg accacacctc tagtgcacgg ccaacgcgtg gcgcagcgat gagcatgaca
     4741 gctcaaccaa cgtggcgccc gctcaaagct gtgctgggcg gtctggtaga cataccagac
     4801 cgcctggagg tttccgacat cacacaggac agtcgcagtg tcatcccggg cgcggccttt
     4861 ctggcctgtc agggccgcac gcagcacggt ctaaagttcg tggacgctgc cgtgtccgcc
     4921 ggtgcacgcg cggtgctgtg ggagcccgct cccggggttg cagcgccggt cgttgacggc
     4981 tcgatcgtcg tgcgtgcggt gcccaatctg catgcgcagc tcggttacat cgccgatcgg
     5041 ttcttcgatg cgccgtcggc cggcatgatg gttgccgcga tcaccggcac caatggcaag
     5101 accacctgcg cctggctgtt ggcgcaggcg ttgaatctgt gcggacgaag cgccgcgtac
     5161 atcgggacgc tcggcttcgg cacacctggc gtgatgcata cgctcgaaca gaccacgccg
     5221 gatgccgtga cgctgcagcg ggcgttggcg acgctacggg ccgcgggaat ggattccatc
     5281 gcgatggagg tctcctcaca cgcgctcgat cagaatcgct gcgccggcat gcggatgcac
     5341 agcgcggtgt tcaccaatct gtcgcgtgat cacctcgact atcacggcga catggacagt
     5401 tacggtgccg ccaaggcgaa actcttcaat tggccgacgc tcgccgcgcg tgtcatcaac
     5461 gtcgatgacc ccttcggcca ggagctggcg cgtgagcgat tacagggtgc gggcgcggtt
     5521 cagtcgcgat tgtttctgac gtcacagcat tccaatgaat ggctcaccag cggcgccgac
     5581 ttcgtgtaca ccgagggcgt tcgggcaagc gcccatggac tggaactgga cgtcgagtcc
     5641 agccgcggcg aaacactgct tcacagtcat ctgcttggcg acttcaatgt cgacaacctg
     5701 ctgacggtgc tggcgacgct gctggcatgg gacattccgc ttgaccaggc gcgcatggcg
     5761 ctggagcgct gcagcgctcc gccgggccgc atgcagcccg atggcggcgg agccttgccg
     5821 ctggtgctgg tggactatgc gcacaccccg gatgcgctgt cgaaggcgct gcacgctgcg
     5881 cgcgcgcact gcgatggccg gctgtggtgc gtgttcggat gcggtggcga acgtgatgtg
     5941 ggcaagcgcg gcctgatggg ccaggtagcg gcgcagctgg cggacgagat cgtcgtgacc
     6001 gatgacaatc cgcgacgcga gaatcccgac cgcattgtcg cggcgatcat ggaaggggtc
     6061 atcgctgccg gcgccggctg gcgcgtgcaa gtcaatcatg atcgcgcgca ggcgattcgt
     6121 gccgcggtca gcaaagcgca tgcgggagac gtggtgctgg tggccggcaa gggtcatgag
     6181 gactatcagc tcatcggcaa cgagcgccgt gagttcagtg acgcggcggt cgtacaccag
     6241 atactggctg agcaggcgcc gcaatgatcc gaacgttgca gcagttcgcc gccgccgccc
     6301 acgggcgtct gctcggcgag gatcgcgaat ttggcgaact cgcgatcgat tcgcgcaaac
     6361 tcagcgctgg ggatctgttc gcggcgctgc gcggtgaaca gctcgacggc catgactacg
     6421 tggccgcggc cgccacagcg ggtgcggccg gcgcgatcgt gcagcgaaaa ctgaaagtag
     6481 cgctgccgca gatcgtcgtg tcgaacgtcg ccacggcgat ggcgcaagcc gcgcgtgccg
     6541 cgcgttcgca gttcaagggc ccggtcatcg gcgtggccgg cagcaacggc aagaccacgg
     6601 tgaaagagat gctgtcggca attctgtcgc agctcggtcc gtgtctgtcc acgcgtggca
     6661 acctgaacaa tcatctgggc gtgccgatga cgctgctgcg attgcaggat acccaccgca
     6721 gcgccgtgat cgagatgggc gccaaccgtc gcggcgacgt cgagcaactg gtgcagatcg
     6781 cccgtcccgg tatcggtctg atcaccaatg cgggcgctga gcatctggaa ggctttggtt
     6841 cgctcgaagg tgccgcgcgc gctgagggtg agatggtggc cgaactgccc agcagcggta
     6901 tcgccgtcat caacgctgac gatgcctatg cgtcgttgtg gcgggccgct acctcggcgc
     6961 aaatagtgac cttcggtctg cgccaggagg cggactttca tccgcgcagt gtgcatttcg
     7021 acgtgaacgc ggaaggtttc atcacgcggt tttgcctgtg cagccggtcc ggcgaagtcg
     7081 atgtcgcatt ggcggtcgcg ggccggcaca acgtgctcaa tgcgctggcc gcagccgctg
     7141 ccgccacgaa tgcgggcgtg acgctcgagc aggtcgcctc gggcctgtcg tcgatgcgcg
     7201 ccgcgaaagg gcgcctgcag ttcaagcgca cccaggcggg tgcctggctg atcgatgatt
     7261 cctacaacgc caatccaagt tcggcgcgcg ccgcgcttga agtcttgagc gagctgccgg
     7321 gacggcgctg gatggtgctc ggcgacatgg cggagctcgg tgcattcgcc gacagctctc
     7381 accgggaaat cggcgagctg gcgcacaacc tcggcgtgga gcggctgtac acgttcggca
     7441 atctggcggc attggccgct gaagcttttg gtccgggcgc ggagcgcttc gccgacgcgg
     7501 ccgctctggc gcaggcattg aatgcagcgc tcacccctga cgtgcgattg ctcatcaaag
     7561 gatcgcgggt caaccgactc gagcgcgtgg tcgaggcttt gtcccgcgct gaaactgcct
     7621 aggaggttaa ggacgtccga tgttgttctg gatcgcgaaa ttgctgtcag tgcactacac
     7681 ggcattccac gtgttctcct acctgacgct gcgagcgatc ctcgcggtga tgtcggcgct
     7741 ggcaatgtcg ctattgatcg gcccctccat gattgcatcg ctgtcacgct accagatcgg
     7801 ccaggtcgtg cgcgatgacg ggccgcagtc gcatctgccg aaggccggta ccccgaccat
     7861 gggcggagca ctgatcatcg tcgtgatcgc tgtgagtacg ctgctgtggg ccgatctgcg
     7921 caaccgcttc gtctggatca cgctggccgt cacggtgggc ttcggtctga tcggcttcta
     7981 cgatgattat ctgaagctgg tcgttcgcaa ctcgaaagga ctgatcccac gcaagaagta
     8041 cttctatcaa agcgtgcttg gcattggggc cgcggtgctg ctgtatcgga cggcacatgc
     8101 gccggccgag acggatttct tcttgccgtt cgtcaagagt gtgcagctac cgctgggcgg
     8161 catcggcttc tgcacgctgg cctacctgat gatcgtcggc atgagcaatg cggtgaacct
     8221 gaccgacggc ctggatgggc ttgcgatcat gccggcggcg atggtcgccg gtgcgctcgg
     8281 tgtgtttgcg tacgcgagcg gcaattctat cttcgccaac tacctgtcga ttccggcgat
     8341 tccgcaggcc ggcgagctgc tcgtattctg cagcgcactg gccggtgccg gattgggatt
     8401 cctttggttc aactcctatc cggcccaggt ttttatgggc gacatcggtg cgctggcgct
     8461 cggggcggca ctgggagtgg tggcaacggt ggtgcgccag gagatcgtgg cactgatcat
     8521 gggcggcatc tttgtgctcg aaaccgcctc ggtgatcctg caggtcgcct ccttcaaact
     8581 gaccgggcgc cgcatcttcc gcatggctcc gatccatcat cacttcgaac tcaagggatg
     8641 ggctgaaccg aaggtgatcg tgcgtttctg gatcatcagc ttcctgctgg tgctcgctgg
     8701 cctgtcaacg ctgaagctgc gatgagtacc gcagcaacca agccgctgag cgcggtggtc
     8761 gtcggcatgg gaaagagtgg ctattcagcc gcgcgctacc tgctggcgcg tggcacgcga
     8821 gtggccgtga ccgacagtcg agcgcaaccc ccggagctgg cgcggctccg atcgctcggc
     8881 gaacagatca cgatgcgcgt gggcggtttc gatgcgtcgt tgctcgagca cgccgacctg
     8941 gtcgtcgtgt caccgggcgt ggcgctgagc ggagcgttct ttgagcaggc acgcgcgcgc
     9001 ggcttgccca tggtcggcga tatagagctg ttcgcgcgcg cggtccgggc cccggtcgca
     9061 ggcatcacgg gcaccaacgg caagagtacg gtgacgacgc tgctggccct gatggcgcag
     9121 aagtcggggc tgcgc
//
LOCUS       DATMRM010000512         5131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_3405618, whole genome shotgun sequence.
ACCESSION   DATMRM010000512 DATMRM010000000
VERSION     DATMRM010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3405618"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..2643)
                     /locus_tag="VNZ06_15655"
     CDS             complement(13..2643)
                     /locus_tag="VNZ06_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWW22236.1"
                     /translation="MEEETRLREDGIGTSSWKFWGPYLSERQWGTVREDYSPHGNAWE
                     YFPHEHARSRAYRWGEDGIGGFSDIEQRLCLALTLWNERDPILKERLFGLTNAEGNHG
                     EDVKELYYYLDATPCHSYLRMLYKYPQDAFPYQQLLDENRRRGLNAPEYELIDTGLFD
                     HDRYFDVFIEYAQVEPGDILLRVTAHNRGPDPAPLHLLPTLWFRNTWSWTPDAPRPEL
                     LYGDPARIEAHHPTLGALHLYADGHPMLLFCDNETNMARLYNYADGPKYPKDGINDFV
                     VSDSDHGIDPANRGTKAAAHYRFNVPAGACVQLRLRLSRELTQTMFEDYDELLAARRK
                     EADGYYSRLQQAIANEDERRVQRQAFAGLIWSKQYYHFDVARWLRGDPTQPTPPSERL
                     NGRNCSWRTLNNADVLSMPDKWEYPWYAAWDLAFHCISFALIDSAFAKHQLVLLTREW
                     YMHPNGQVPAYEWAFGDVNPPVHAWAAWRVYQIDRNHNNGLADHDFLERVFHKLLLNF
                     TWWVNRKDAHGRNVFQGGFLGLDNIGAFDRSSQLPQGGELDQSDGTAWMAMYSLNLLR
                     ISLELARSNHVYEDIATKFFEHFLQIAEAMSGMGGERVELWDDEDEFFYDVLHMPDGE
                     QIRMKVRSMVGLIPLFAVETLEPELLNELPHFKARLEWYLQHRPELAALVSRWNERGR
                     GDRRMLSLLRGHRMKALLRRMLDETEFLSDYGVRGISKVHAAHPYVLRLDGHDLCVRY
                     QPAESDSNLFGGNSNWRGPIWFPLNFLLIESLQKFHHYYGNDFTVECPQRSGRYLTLE
                     QIAAELSARLARLFLRDAHGRRPVFGDVDKLQQDPNFRDCLQFFEYFHGDNGRGVGAS
                     HQTGWTGLVAKLLAPRRHEQ"
     gene            complement(2653..4680)
                     /locus_tag="VNZ06_15660"
     CDS             complement(2653..4680)
                     /locus_tag="VNZ06_15660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HWW22237.1"
                     /translation="MTNATPFGREICGNLEQSLHREWLVANGLGGFACGTIAGCQTRR
                     YHGLLIAAERPPVGRTLYLVDLDAMARVDGRQYELGCHEYEGGMVNPHGYRFIESFRL
                     DGTVPTWVYALGSARLRKRVFMARERNTTYVTYTLERSLAPITLTVRPLCTGRDYHWQ
                     RRGGDGYHCDTIAGGCRIIAAGSTGRVDITADAGEFVAAPDMHWNLRHRVEAERGLDS
                     TEDLYMPGVFEFTLSQGDRIHFTATIETGAEPADVALNSLLRHEATLLAPVNAVQPRW
                     IRQLVLAADQYIVARPGTPKGKTVIAGYPWFSDWGRDTMIALPGLTLSTGRTDIAVDI
                     LRTFARYVSQGMLPNRFPDSGEAPEYNTVDATLWYFVAIHEYLRATTDIAFAHEIYPV
                     LEEIVAWHRRGTRHGIGVDPSDGLLRFGEPGTQLTWMDAKVDQWVVTPRAGKAVEINA
                     LWCNALWIMGQLAEQIGNTREARSYDDAAHATAQVFGRRFWFEDGQYLYDVIDATDGP
                     SLDTSLRPNQLIALSLPYRILDDVRARMVLRTCERELLTSYGLRTLDPDDPRYVGRYG
                     GNQRQRDGSYHQGTVWPWLLGAFARAHFSLYGNVIWAASYLSPLEQHLADACIGQVSE
                     IFDADAPHLPQGCFAQAWSVAEVLRAWLEIHAAASLSGAATEAANLARKVS"
     gene            complement(4774..5127)
                     /locus_tag="VNZ06_15665"
     CDS             complement(4774..5127)
                     /locus_tag="VNZ06_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012277143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HWW22238.1"
                     /translation="MRAIASTVGLLCLSNVFMTFAWYAHLKELYQKPWIVAALVSWGI
                     ALFEYLLQVPGNRIGFAVLNVAQLKILQEVITLAVFVPFAVLYMKEPLKLDYLWAALC
                     ILGAVFFVFRGKLAA"
BASE COUNT         1046 a         1560 c         1517 g         1008 t
ORIGIN      
        1 attcggcgcg agttattgct cgtgccttcg cggcgccaac agtttcgcga ccagacccgt
       61 ccagccggtc tgatgcgagg cgccaacccc gcggccgtta tcgccgtgaa agtactcgaa
      121 gaactgcagg caatcgcgga aattcggatc ctgctgcaac ttgtcgacgt ctccgaacac
      181 cggcctgcga ccgtgcgcat cacgcagaaa cagcctcgcc aggcgtgcgg aaagttctgc
      241 ggcaatctgc tccaaggtca ggtagcgtcc cgatcgttgc ggacattcga ccgtgaaatc
      301 gttgccgtag tagtgatgaa acttctgcag cgattcgatc agcaaaaagt tcaacgggaa
      361 ccagatcggt ccgcgccagt tcgagttgcc gccaaacaga ttcgagtctg actcggctgg
      421 ctgatagcgc acgcacaaat cgtgaccgtc gagtcgcagc acgtagggat gcgcggcatg
      481 caccttggag attccgcgca cgccgtagtc ggacagaaac tcggtctcat ccagcatccg
      541 gcgcagcaag gccttcatgc ggtggccgcg caacagcgag agcatgcgtc gatcgccgcg
      601 gcctctttca ttccaacgcg acaccagggc tgccagctcg ggccggtgct gcagatacca
      661 ttcgagccga gccttgaagt gcggcagctc gttcaatagc tccggttcga gcgtctccac
      721 cgcgaacagt ggaatgagtc cgaccatcga gcgcactttc atccggatct gctcaccgtc
      781 cggcatatgt agcacgtcat agaagaattc gtcttcgtca tcccatagtt caacgcgctc
      841 gccgcccatg ccgctcatgg cttcagcaat ttgcagaaaa tgctcgaaga acttggtcgc
      901 gatatcctcg tagacgtggt tgctgcgcgc cagctccagc gagatgcgca acagattcag
      961 cgaatacatc gccatccagg cggtgccgtc ggactggtcg agttcgcctc cctgcggtag
     1021 ctgcgagctg cgatcgaacg caccgatatt gtcgagcccc aggaacccgc cctggaacac
     1081 gttgcgtccg tgtgcgtctt tgcgattgac ccaccaggtg aagttcagca gcagcttgtg
     1141 gaatacgcgt tccaggaaat catgatcggc gagcccattg ttgtgattgc ggtcgatctg
     1201 atacacgcgc cacgccgccc aggcgtgcac cggaggattg acgtcgccaa aggcccattc
     1261 gtacgccggt acctggccgt tggggtgcat gtaccactcg cgtgttaaca acacgagctg
     1321 gtgtttcgca aacgccgaat caatcagagc gaaggaaatg caatgaaacg ccaaatccca
     1381 tgcggcatac cagggatact cccacttgtc cggcatcgac aggacatcgg cattgttcag
     1441 ggttcgccag ctgcagttgc gtccgttgag gcgctcggat ggtggtgtgg gctgcgtcgg
     1501 gtcacctcgt agccatcgag cgacgtcaaa gtggtagtac tgcttgctcc agatgagtcc
     1561 ggcgaatgcc tggcgttgga cgcgacgttc atcctcgttt gcaatagcct gctgcagcct
     1621 cgaatagtag ccgtcggctt ccttccgccg agccgccagc agttcatcgt aatcctcaaa
     1681 catggtctgc gtgagttcgc gcgagagccg caagcgaagc tgcacgcagg cgccggccgg
     1741 cacgttgaat cgatagtgag ccgccgcttt ggtgccgcga ttggcaggat cgatgccgtg
     1801 gtcactgtcg cttacgacga aatcgttgat accgtccttg gggtactttg ggccatccgc
     1861 atagttgtac agccgcgcca tattcgtttc gttgtcacag aacagcagca tcggatgccc
     1921 atcggcatag agatgtagcg cgcccagcgt ggggtgatgc gcttcgattc gcgccggatc
     1981 cccatagagc aactccggac gcggcgcgtc gggagtccag ctccaggtgt ttctgaacca
     2041 gagggtcggt aacagatgca gcggtgccgg atccgggccg cggttgtgag cggtgacgcg
     2101 cagcaggatg tcaccgggtt cgacttgcgc atattcgatg aagacatcga agtagcgatc
     2161 gtgatcgaac aggcccgtgt cgatcagctc gtactcggga gcattcaatc cccgacgccg
     2221 attctcgtcc aataattgct ggtaaggaaa ggcgtcctgc ggatacttgt agagcatccg
     2281 caggtaggag tgacagggcg tggcatccag gtagtagtag agctccttga cgtcttcgcc
     2341 gtgattgccc tcggcattgg tcagcccgaa gaggcgctcc ttgagaattg gatcgcgctc
     2401 attccaaagt gtcagcgcca ggcacagacg ctgttcgatg tcgctgaaac cgccgatgcc
     2461 gtcctcaccc cagcgatagg cgcgactgcg tgcgtgttca tgtgggaaat attcccaggc
     2521 attgccgtga gggctgtagt cctctcggac cgtgccccat tgccgttcgc ttaagtaagg
     2581 tccccaaaac ttccagctcg aagtgccgat cccatcctca cgtaagcgtg tctcttcttc
     2641 catccattgc ccctaggaga ccttgcgagc gagattggcg gcttccgtgg cggcgcccga
     2701 aaggctcgcg gccgcatgaa tttcgagcca ggcgcgcagc acttcggcga cgctccacgc
     2761 ctgagcaaag catccttgcg gcagatgtgg ggcatccgcg tcgaatatct cgctgacctg
     2821 tccaatacag gcgtctgcca ggtgttgttc cagcggtgac agataagagg ccgcccagat
     2881 cacgttgccg tacagactga aatgcgcgcg tgcgaatgca ccgagcagcc acggccagac
     2941 ggtgccctga tgataggagc catcacgctg gcgttggttg ccgccatacc ggcctacgta
     3001 gcgtgggtcg tccgggtcga gcgtgcgcag cccataggat gtaagcaatt cgcgctcgca
     3061 ggtccgaagg accattcgtg cacgcacgtc atccagaatt ctgtagggca gcgacagtgc
     3121 aatcagttga ttgggtcgca atgacgtgtc gagcgagggc ccgtcagtgg cgtcgatgac
     3181 atcgtagaga tactgtccat cctcgaacca gaagcgtcga ccgaagacct gcgcggtcgc
     3241 atgggctgcg tcgtcgtagc tgcgtgcttc gcgcgtgttg ccgatctgtt cagccagttg
     3301 ccccatgatc cacaacgcgt tacaccacag cgcgttgatc tcgaccgcct tgccggcgcg
     3361 cggtgtaacg acccactgat cgaccttggc gtccatccag gtcagttggg tccctggctc
     3421 tccgaagcgt agcaaaccat ccgatggatc aacaccaatg ccgtgtcgcg taccgcgacg
     3481 atgccaggca acgatttcct cgagcaccgg atagatttca tgagcgaacg cgatatcggt
     3541 ggttgctctc aagtattcgt ggatcgcgac gaaataccac agcgtggcat cgacggtgtt
     3601 gtattcgggt gcctcacccg aatccggaaa acgatttggc aacatgccct ggctgacgta
     3661 ccgggcaaag gttcgcagaa tgtcaacggc aatatcggtg cgtccggttg agagcgtcag
     3721 ccccggcagt gcgatcatcg tgtcgcgtcc ccagtcacta aaccagggat agccggcgat
     3781 caccgttttc cccttggggg tgcccggtct ggcaacgatg tattgatccg cggccagtac
     3841 cagctggcgg atccatcgcg gctgcacggc gttcaccggt gccagcaacg tcgcctcgtg
     3901 tcgcaacaaa gaattgagcg cgacatccgc cggctcggcg ccggtttcga tcgtggcggt
     3961 gaagtggatc cgatcaccct gcgagagcgt gaactcgaaa acaccgggca tgtacagatc
     4021 ttccgtgctg tccagacctc gttcggcctc gacccggtgc cgcaggttcc aatgcatgtc
     4081 gggcgcggcg acaaactcgc cggcatcggc cgtgatgtca actctccctg tactcccggc
     4141 ggcgatgatg cggcagcccc cggcgatcgt atcgcagtga tagccgtcac cgccgcggcg
     4201 ttgccagtgg tagtcgcgtc cagtacacag cgggcgcacc gtcagcgtga tcggagccag
     4261 cgaacgctcc agcgtatagg taacataggt cgtattccgc tcgcgcgcca tgaagacacg
     4321 tttccgcagg cgagccgatc ccagcgcata gacccaggtg ggcaccgtcc cgtcgaggcg
     4381 gaagctctcg atgaatcgat agccgtgggg attgaccatg ccaccctcat attcgtggca
     4441 gccgagctcg tactgtcggc cgtctacgcg ggccatcgca tccaggtcca ccagatagag
     4501 ggtgcgtccg accggaggcc tttcggccgc gatcagcaga ccgtggtagc gacgcgtctg
     4561 gcagccggca atcgtcccgc aggcaaaccc acccaatcca ttggcgacca gccactcgcg
     4621 atgcagcgac tgctcgagat tgccgcagat ttccctgcca aacggggtag cgtttgtcat
     4681 cagtcaactc cagaagcgga cggtgcgact tcgagtggac gctgccgtcg catcataccg
     4741 aacctcgggt tgcccggtcg tgaccaacgc caactacgcg gccagcttgc ccctaaatac
     4801 gaaaaaaacg gctcccagaa tgcacagcgc cgcccacagg tagtcgagtt tcagcggttc
     4861 tttcatatag agcaccgcaa acggcacgaa taccgcgagc gtgatgacct cctgcagaat
     4921 cttcagctgc gcgacattga gcaccgcgaa tccgatgcgg ttgccgggaa cctgaagcag
     4981 atactcgaac aatgcaattc cccagctcac cagtgccgct acgatccacg gtttctggta
     5041 cagctccttg agatgggcgt accaggcaaa ggtcatgaag acgttgctaa ggcacagcag
     5101 gccgacggtc gatgcaatcg cgcgcatgaa a
//
LOCUS       DATMRM010000513         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U12955
            k141_301005, whole genome shotgun sequence.
ACCESSION   DATMRM010000513 DATMRM010000000
VERSION     DATMRM010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943561
            Sequence Read Archive: SRR7102016
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 19:21:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,135
            CDSs (total)                      :: 3,099
            Genes (coding)                    :: 3,082
            CDSs (with protein)               :: 3,082
            Genes (RNA)                       :: 36
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 16 of 17
            Pseudo Genes (internal stop)      :: 0 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301005"
                     /isolate="SMAG_U12955"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VNZ06_15670"
     CDS             <1..773
                     /locus_tag="VNZ06_15670"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HWW22239.1"
                     /translation="NGYAQVLRRLDSTLQIDIVTAADAAPRFPQGAGISDYDGVTMTG
                     SALNIYHGGTAVTRQIELAKAVFEAGVPFFGSCWGLQVAVTAAGGEVRANPRGREFGF
                     ARRVLLNEAGRSHRLFAGKPVEFEAPTIHRDEIASLPTGAQVLAANDFGVQAAAFRFG
                     RGIVWGVQYHPEYDYADIAAAAERYGETLVIEGMFRDAQGLSAFAAELRALQSNPADA
                     VLLWKHGLGAAMRTAELRLLELRNWLDAEVRSRAARHV"
     gene            766..>2057
                     /gene="eat"
                     /locus_tag="VNZ06_15675"
     CDS             766..>2057
                     /gene="eat"
                     /locus_tag="VNZ06_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HWW22240.1"
                     /translation="MSDSTPALKKALSGPHLWGIAVGLVISGEYFGWSYGWAHAGTLG
                     FLVTTVFIALMYTTFIFSFTELTTAIPHAGGPFAYSYRAFGAIGGHIAGFSTLIEFVF
                     APPAIALAIGAYLNVQFPALDPRTAATSAYLVFMALNIAGVTIAATFELVVTVLAIIE
                     LLIFMAVVSGGFAWSNFANHGWGGADHASAATLAGIFAAIPFAIWFFLAIEGAAMAAE
                     EARDPARTIPRAYIGGILTLVVLAFGTMIFAGAVGNWTALANINDPLPQAMKIVVGER
                     SGWLHLLVWIGVLGLIASFHGIILGYSRQIFALARAGLLPRSLASVHPRWRTPHVAIL
                     AGGVVGIAAIYSDRFVTIAGQPLTASIVTMSALGAVVMYITSLLSLMRLRRAEPRLVR
                     PFRVPLYPLFPMIALSIAIVSLIAIVYYNAMVATVFAAL"
BASE COUNT          329 a          659 c          639 g          430 t
ORIGIN      
        1 gcaatggcta cgcgcaggtg ctacggcgcc tcgattcgac gctgcagatc gacattgtga
       61 ccgcagccga cgctgctccc cgatttccgc aaggtgccgg cattagcgac tatgacggcg
      121 tgaccatgac cgggtcagca ctcaacatct atcatggtgg cactgcggtg acgcgtcaga
      181 tcgaactggc caaagcagtc ttcgaggcgg gtgtgccatt cttcggcagc tgctggggat
      241 tgcaggtcgc cgtgaccgct gcgggcggcg aggtgcgcgc caacccccgc ggccgcgagt
      301 ttgggttcgc ccggcgggtg ctactgaacg aggccggccg ttcgcatcga ttgttcgccg
      361 gcaagccggt cgagttcgag gcacccacca ttcaccgcga cgagattgca tcgctaccga
      421 ccggcgccca ggtactcgcg gccaacgact tcggcgtgca ggcggcggca ttccgcttcg
      481 gccgcggcat agtctggggt gtgcaatacc atccggagta cgactacgcg gacattgcag
      541 cggccgccga acgctacggt gagacgctgg tgatcgaagg gatgtttcgc gatgcgcagg
      601 gcttaagcgc gtttgcggca gaactgcgtg ccctgcagtc gaatccagcg gacgcggtac
      661 tgctctggaa acatgggctg ggagcggcta tgcgcaccgc ggagctccgg ctgctggaac
      721 tacgcaactg gctcgacgcc gaggtgcgct cgcgggcggc gcgtcatgtc tgacagcacc
      781 cccgctctca agaaggcact gagcggccct cacctttggg gcatcgccgt cggactggtg
      841 atttcgggcg aatactttgg ctggagctac ggctgggcgc atgccggcac tctgggcttt
      901 ctggtcacga ccgtgttcat cgcgctgatg tatacgacct tcatattcag cttcacggag
      961 ctcacgaccg cgatcccgca tgcaggaggc ccgttcgcct acagctaccg cgcattcggc
     1021 gccatcggtg gtcatatcgc cgggttctcg acgctgatcg agtttgtctt cgcgccgccg
     1081 gcgatcgcgc tggcgatcgg cgcctatctc aacgtgcagt tcccggcgct ggatcctcgc
     1141 accgccgcca ccagcgccta tctcgttttc atggccctca acatcgccgg ggtaaccatt
     1201 gccgctacct tcgaactggt cgtgaccgta ctcgcgatca tcgaactgct gatcttcatg
     1261 gcggtcgtgt ccggcggttt tgcctggtcc aacttcgcga accacggctg gggaggtgca
     1321 gaccacgcgt cggctgcgac gctcgccggc atctttgctg cgatcccatt cgcgatctgg
     1381 ttctttctgg caatcgaggg tgcggcgatg gccgccgagg aagccaggga tcccgcgcgc
     1441 accattccga gagcttatat cggcggcatt ctcacgctgg tggtccttgc attcggcacc
     1501 atgatctttg ccggcgccgt cggtaattgg acggcactcg ccaacatcaa cgacccgtta
     1561 ccgcaggcga tgaagatcgt ggtcggcgaa cgcagcggct ggctgcacct gctggtatgg
     1621 attggcgtgc tcggcctgat tgcctcattc cacggcatca tcctgggtta ttcccggcag
     1681 atctttgcgc tggcacgcgc cggattgctg ccacgatcgc tcgcctcggt tcacccgcgg
     1741 tggcgtacac cgcacgtcgc gattctggcc ggtggcgtcg ttggcattgc agccatttat
     1801 tcggacaggt ttgttacgat cgccggacaa ccactgactg ccagtatcgt cacgatgtcc
     1861 gcactgggcg ctgtcgtgat gtacatcacg agcctgctga gcctcatgcg tctgcgacgc
     1921 gcggaacctc gactcgtgcg tccgtttcgc gtgcccctct acccgctatt tccgatgatc
     1981 gcgctcagca tcgcaatcgt gtctctcatt gcaatcgtct actacaacgc gatggtggcg
     2041 acagtgttcg cagccct
//
